LOCUS       DAPHJE010000001       146719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPHJE010000001 DAPHJE010000000
VERSION     DAPHJE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 146719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 146719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..146719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.001"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            128..3079
                     /locus_tag="RH222_000001"
     CDS             128..3079
                     /locus_tag="RH222_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3795078.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGSITSELPFGYYSITPEGYNGYTFNNGTTTDLDYNWY
                     DGDVFTLNVSNSTIDDDYEALYFTDTHLDGDKSKSTNETFYTALGVAVNLDVESNINI
                     TNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTPLEESDGFFGKSA
                     EPSDYAGNGGQNDVALSFTDNAGSNYSMKNNVNFDHSTLLGDVEFTSHWNNDGVFFYS
                     TGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAEVIAPADMYDVAP
                     NSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNSSEWNTVNSSVIDTLAVNNGSQVN
                     VTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSYSTVNLTESTGWNNYSNLYTNT
                     ITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNADL
                     VNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVR
                     IDNATGAGTIADYKDKELIRVNDANTNATFSAANKADLGAYTYQAEQRGNTVVLQQME
                     LTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGT
                     INYDQDVNGIMVGVDTKVDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSA
                     HFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYV
                     TPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
     gene            3167..3790
                     /gene="iprA"
                     /locus_tag="RH222_000002"
     CDS             3167..3790
                     /gene="iprA"
                     /locus_tag="RH222_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV3795079.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(3791..4948)
                     /gene="ampH"
                     /locus_tag="RH222_000003"
     CDS             complement(3791..4948)
                     /gene="ampH"
                     /locus_tag="RH222_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV3795080.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKQLVIPAS"
     gene            5300..6520
                     /gene="sbmA"
                     /locus_tag="RH222_000004"
     CDS             5300..6520
                     /gene="sbmA"
                     /locus_tag="RH222_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV3795081.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6533..7627
                     /gene="yaiW"
                     /locus_tag="RH222_000005"
     CDS             6533..7627
                     /gene="yaiW"
                     /locus_tag="RH222_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV3795082.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVGSVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(7686..7994)
                     /locus_tag="RH222_000006"
     CDS             complement(7686..7994)
                     /locus_tag="RH222_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV3795083.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            8254..8466
                     /locus_tag="RH222_000007"
     CDS             8254..8466
                     /locus_tag="RH222_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV3795084.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(8490..9584)
                     /gene="ddlA"
                     /locus_tag="RH222_000008"
     CDS             complement(8490..9584)
                     /gene="ddlA"
                     /locus_tag="RH222_000008"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV3795085.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            9662..9865
                     /locus_tag="RH222_000009"
     CDS             9662..9865
                     /locus_tag="RH222_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795086.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            10047..10307
                     /gene="iraP"
                     /locus_tag="RH222_000010"
     CDS             10047..10307
                     /gene="iraP"
                     /locus_tag="RH222_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV3795087.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            10408..11823
                     /gene="phoA"
                     /locus_tag="RH222_000011"
     CDS             10408..11823
                     /gene="phoA"
                     /locus_tag="RH222_000011"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV3795088.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            11942..12262
                     /gene="psiF"
                     /locus_tag="RH222_000012"
     CDS             11942..12262
                     /gene="psiF"
                     /locus_tag="RH222_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV3795089.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            12364..13479
                     /gene="adrA"
                     /locus_tag="RH222_000013"
                     /gene_synonym="dgcC"
     CDS             12364..13479
                     /gene="adrA"
                     /locus_tag="RH222_000013"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV3795090.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(13496..14305)
                     /gene="proC"
                     /locus_tag="RH222_000014"
     CDS             complement(13496..14305)
                     /gene="proC"
                     /locus_tag="RH222_000014"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV3795091.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            14425..14889
                     /locus_tag="RH222_000015"
     CDS             14425..14889
                     /locus_tag="RH222_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV3795092.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            15066..15590
                     /gene="aroL"
                     /locus_tag="RH222_000016"
     CDS             15066..15590
                     /gene="aroL"
                     /locus_tag="RH222_000016"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV3795093.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            15640..15831
                     /gene="yaiA"
                     /locus_tag="RH222_000017"
     CDS             15640..15831
                     /gene="yaiA"
                     /locus_tag="RH222_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV3795094.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            16089..16766
                     /locus_tag="RH222_000018"
     CDS             16089..16766
                     /locus_tag="RH222_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV3795095.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            16838..17122
                     /gene="ppnP"
                     /locus_tag="RH222_000019"
     CDS             16838..17122
                     /gene="ppnP"
                     /locus_tag="RH222_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV3795096.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            17277..17612
                     /locus_tag="RH222_000020"
                     /pseudo
     CDS             17277..17612
                     /locus_tag="RH222_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            17609..17692
                     /locus_tag="RH222_000021"
     CDS             17609..17692
                     /locus_tag="RH222_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795097.1"
                     /translation="MTQRPWSKLQRKKHNIAALKIIARRSE"
     gene            complement(17770..18681)
                     /gene="rdgC"
                     /locus_tag="RH222_000022"
     CDS             complement(17770..18681)
                     /gene="rdgC"
                     /locus_tag="RH222_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV3795098.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            18806..19714
                     /gene="mak"
                     /locus_tag="RH222_000023"
     CDS             18806..19714
                     /gene="mak"
                     /locus_tag="RH222_000023"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV3795099.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(19857..21041)
                     /gene="araJ"
                     /locus_tag="RH222_000024"
     CDS             complement(19857..21041)
                     /gene="araJ"
                     /locus_tag="RH222_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV3795100.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYMMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(21167..24310)
                     /gene="sbcC"
                     /locus_tag="RH222_000025"
     CDS             complement(21167..24310)
                     /gene="sbcC"
                     /locus_tag="RH222_000025"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV3795101.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(24307..25509)
                     /gene="sbcD"
                     /locus_tag="RH222_000026"
     CDS             complement(24307..25509)
                     /gene="sbcD"
                     /locus_tag="RH222_000026"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV3795102.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            25699..26388
                     /gene="phoB"
                     /locus_tag="RH222_000027"
     CDS             25699..26388
                     /gene="phoB"
                     /locus_tag="RH222_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV3795103.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            26446..27741
                     /gene="phoR"
                     /locus_tag="RH222_000028"
     CDS             26446..27741
                     /gene="phoR"
                     /locus_tag="RH222_000028"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV3795104.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLGGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            28148..29467
                     /gene="brnQ"
                     /locus_tag="RH222_000029"
     CDS             28148..29467
                     /gene="brnQ"
                     /locus_tag="RH222_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV3795105.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            29543..30916
                     /gene="proY"
                     /locus_tag="RH222_000030"
     CDS             29543..30916
                     /gene="proY"
                     /locus_tag="RH222_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV3795106.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            31072..32889
                     /gene="malZ"
                     /locus_tag="RH222_000031"
     CDS             31072..32889
                     /gene="malZ"
                     /locus_tag="RH222_000031"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV3795107.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(32894..33475)
                     /gene="acpH"
                     /locus_tag="RH222_000032"
     CDS             complement(32894..33475)
                     /gene="acpH"
                     /locus_tag="RH222_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV3795108.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            33568..34638
                     /gene="queA"
                     /locus_tag="RH222_000033"
     CDS             33568..34638
                     /gene="queA"
                     /locus_tag="RH222_000033"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV3795109.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            34694..35821
                     /gene="tgt"
                     /locus_tag="RH222_000034"
     CDS             34694..35821
                     /gene="tgt"
                     /locus_tag="RH222_000034"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV3795110.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            35844..36176
                     /gene="yajC"
                     /locus_tag="RH222_000035"
     CDS             35844..36176
                     /gene="yajC"
                     /locus_tag="RH222_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV3795111.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            36204..38051
                     /gene="secD"
                     /locus_tag="RH222_000036"
     CDS             36204..38051
                     /gene="secD"
                     /locus_tag="RH222_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV3795112.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            38062..39033
                     /gene="secF"
                     /locus_tag="RH222_000037"
     CDS             38062..39033
                     /gene="secF"
                     /locus_tag="RH222_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV3795113.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            39162..39509
                     /locus_tag="RH222_000038"
     CDS             39162..39509
                     /locus_tag="RH222_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV3795114.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(39686..40570)
                     /gene="tsx"
                     /locus_tag="RH222_000039"
     CDS             complement(39686..40570)
                     /gene="tsx"
                     /locus_tag="RH222_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV3795115.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINDIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(40869..41408)
                     /locus_tag="RH222_000040"
     CDS             complement(40869..41408)
                     /locus_tag="RH222_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV3795116.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            41559..42008
                     /gene="nrdR"
                     /locus_tag="RH222_000041"
     CDS             41559..42008
                     /gene="nrdR"
                     /locus_tag="RH222_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV3795117.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            42012..43115
                     /gene="ribD"
                     /locus_tag="RH222_000042"
     CDS             42012..43115
                     /gene="ribD"
                     /locus_tag="RH222_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV3795118.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            43204..43674
                     /gene="ribE"
                     /locus_tag="RH222_000043"
     CDS             43204..43674
                     /gene="ribE"
                     /locus_tag="RH222_000043"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV3795119.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            43694..44113
                     /gene="nusB"
                     /locus_tag="RH222_000044"
     CDS             43694..44113
                     /gene="nusB"
                     /locus_tag="RH222_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV3795120.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            44191..45168
                     /gene="thiL"
                     /locus_tag="RH222_000045"
     CDS             44191..45168
                     /gene="thiL"
                     /locus_tag="RH222_000045"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV3795121.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            45146..45664
                     /gene="pgpA"
                     /locus_tag="RH222_000046"
     CDS             45146..45664
                     /gene="pgpA"
                     /locus_tag="RH222_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV3795122.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(45718..46692)
                     /gene="yajO"
                     /locus_tag="RH222_000047"
     CDS             complement(45718..46692)
                     /gene="yajO"
                     /locus_tag="RH222_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV3795123.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSFSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(46872..48734)
                     /gene="dxs"
                     /locus_tag="RH222_000048"
     CDS             complement(46872..48734)
                     /gene="dxs"
                     /locus_tag="RH222_000048"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV3795124.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(48759..49658)
                     /gene="ispA"
                     /locus_tag="RH222_000049"
     CDS             complement(48759..49658)
                     /gene="ispA"
                     /locus_tag="RH222_000049"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV3795125.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(49658..49900)
                     /gene="xseB"
                     /locus_tag="RH222_000050"
     CDS             complement(49658..49900)
                     /gene="xseB"
                     /locus_tag="RH222_000050"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV3795126.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            50106..51554
                     /gene="thiI"
                     /locus_tag="RH222_000051"
     CDS             50106..51554
                     /gene="thiI"
                     /locus_tag="RH222_000051"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV3795127.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(51608..52198)
                     /gene="yajL"
                     /locus_tag="RH222_000052"
     CDS             complement(51608..52198)
                     /gene="yajL"
                     /locus_tag="RH222_000052"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV3795128.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(52161..53072)
                     /gene="panE"
                     /locus_tag="RH222_000053"
     CDS             complement(52161..53072)
                     /gene="panE"
                     /locus_tag="RH222_000053"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV3795129.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            53240..53731
                     /gene="yajQ"
                     /locus_tag="RH222_000054"
     CDS             53240..53731
                     /gene="yajQ"
                     /locus_tag="RH222_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV3795130.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(53859..55223)
                     /locus_tag="RH222_000055"
     CDS             complement(53859..55223)
                     /locus_tag="RH222_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3795131.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFSLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(55372..56262)
                     /gene="cyoE"
                     /locus_tag="RH222_000056"
     CDS             complement(55372..56262)
                     /gene="cyoE"
                     /locus_tag="RH222_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV3795132.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(56274..56603)
                     /locus_tag="RH222_000057"
     CDS             complement(56274..56603)
                     /locus_tag="RH222_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV3795133.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(56603..57217)
                     /locus_tag="RH222_000058"
     CDS             complement(56603..57217)
                     /locus_tag="RH222_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV3795134.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(57207..59198)
                     /gene="cyoB"
                     /locus_tag="RH222_000059"
     CDS             complement(57207..59198)
                     /gene="cyoB"
                     /locus_tag="RH222_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV3795135.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(59220..60167)
                     /gene="cyoA"
                     /locus_tag="RH222_000060"
     CDS             complement(59220..60167)
                     /gene="cyoA"
                     /locus_tag="RH222_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV3795136.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(60627..62102)
                     /gene="ampG"
                     /locus_tag="RH222_000061"
     CDS             complement(60627..62102)
                     /gene="ampG"
                     /locus_tag="RH222_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV3795137.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(62146..62724)
                     /locus_tag="RH222_000062"
     CDS             complement(62146..62724)
                     /locus_tag="RH222_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3795138.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            63029..63346
                     /gene="bolA"
                     /locus_tag="RH222_000063"
     CDS             63029..63346
                     /gene="bolA"
                     /locus_tag="RH222_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV3795139.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            63690..64988
                     /gene="tig"
                     /locus_tag="RH222_000064"
     CDS             63690..64988
                     /gene="tig"
                     /locus_tag="RH222_000064"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV3795140.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            65234..65857
                     /gene="clpP"
                     /locus_tag="RH222_000065"
     CDS             65234..65857
                     /gene="clpP"
                     /locus_tag="RH222_000065"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV3795141.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            65983..67257
                     /gene="clpX"
                     /locus_tag="RH222_000066"
     CDS             65983..67257
                     /gene="clpX"
                     /locus_tag="RH222_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV3795142.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            67445..69799
                     /gene="lon"
                     /locus_tag="RH222_000067"
     CDS             67445..69799
                     /gene="lon"
                     /locus_tag="RH222_000067"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV3795143.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            70008..70280
                     /gene="hupB"
                     /locus_tag="RH222_000068"
     CDS             70008..70280
                     /gene="hupB"
                     /locus_tag="RH222_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV3795144.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            70472..72343
                     /gene="ppiD"
                     /locus_tag="RH222_000069"
     CDS             70472..72343
                     /gene="ppiD"
                     /locus_tag="RH222_000069"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV3795145.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQTAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            72494..72865
                     /locus_tag="RH222_000070"
     CDS             72494..72865
                     /locus_tag="RH222_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV3795146.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            72959..73357
                     /gene="fadM"
                     /locus_tag="RH222_000071"
     CDS             72959..73357
                     /gene="fadM"
                     /locus_tag="RH222_000071"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV3795147.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(73409..74104)
                     /gene="queC"
                     /locus_tag="RH222_000072"
     CDS             complement(73409..74104)
                     /gene="queC"
                     /locus_tag="RH222_000072"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV3795148.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(74169..75869)
                     /locus_tag="RH222_000073"
     CDS             complement(74169..75869)
                     /locus_tag="RH222_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV3795149.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            75969..76787
                     /gene="cof"
                     /locus_tag="RH222_000074"
     CDS             75969..76787
                     /gene="cof"
                     /locus_tag="RH222_000074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV3795150.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            76940..77398
                     /gene="decR"
                     /locus_tag="RH222_000075"
     CDS             76940..77398
                     /gene="decR"
                     /locus_tag="RH222_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV3795151.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            77428..79200
                     /locus_tag="RH222_000076"
     CDS             77428..79200
                     /locus_tag="RH222_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV3795152.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            79193..80974
                     /locus_tag="RH222_000077"
     CDS             79193..80974
                     /locus_tag="RH222_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV3795153.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            81155..81493
                     /gene="glnK"
                     /locus_tag="RH222_000078"
     CDS             81155..81493
                     /gene="glnK"
                     /locus_tag="RH222_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV3795154.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            81523..82809
                     /gene="amtB"
                     /locus_tag="RH222_000079"
     CDS             81523..82809
                     /gene="amtB"
                     /locus_tag="RH222_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV3795155.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(82858..83718)
                     /gene="tesB"
                     /locus_tag="RH222_000080"
     CDS             complement(82858..83718)
                     /gene="tesB"
                     /locus_tag="RH222_000080"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV3795156.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            83936..84508
                     /locus_tag="RH222_000081"
     CDS             83936..84508
                     /locus_tag="RH222_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDV3795157.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(84539..84850)
                     /locus_tag="RH222_000082"
     CDS             complement(84539..84850)
                     /locus_tag="RH222_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV3795158.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            85013..85109
                     /gene="ffs"
                     /locus_tag="RH222_000083"
     ncRNA           85013..85109
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RH222_000083"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            85229..85582
                     /locus_tag="RH222_000084"
     CDS             85229..85582
                     /locus_tag="RH222_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDV3795159.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(85624..87174)
                     /gene="pdeB"
                     /locus_tag="RH222_000085"
     CDS             complement(85624..87174)
                     /gene="pdeB"
                     /locus_tag="RH222_000085"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV3795160.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(87338..87808)
                     /locus_tag="RH222_000086"
     CDS             complement(87338..87808)
                     /locus_tag="RH222_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV3795161.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(87924..88474)
                     /gene="maa"
                     /locus_tag="RH222_000087"
                     /pseudo
     CDS             complement(87924..88474)
                     /gene="maa"
                     /locus_tag="RH222_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(88646..88864)
                     /gene="hha"
                     /locus_tag="RH222_000088"
     CDS             complement(88646..88864)
                     /gene="hha"
                     /locus_tag="RH222_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV3795162.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(88890..89264)
                     /gene="tomB"
                     /locus_tag="RH222_000089"
     CDS             complement(88890..89264)
                     /gene="tomB"
                     /locus_tag="RH222_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV3795163.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(89810..92959)
                     /locus_tag="RH222_000090"
     CDS             complement(89810..92959)
                     /locus_tag="RH222_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV3795164.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(92982..94175)
                     /gene="acrA"
                     /locus_tag="RH222_000091"
     CDS             complement(92982..94175)
                     /gene="acrA"
                     /locus_tag="RH222_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV3795165.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            94317..94964
                     /gene="acrR"
                     /locus_tag="RH222_000092"
     CDS             94317..94964
                     /gene="acrR"
                     /locus_tag="RH222_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV3795166.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            95092..98454
                     /gene="mscK"
                     /locus_tag="RH222_000093"
     CDS             95092..98454
                     /gene="mscK"
                     /locus_tag="RH222_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV3795167.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(98666..98827)
                     /gene="rsmS"
                     /locus_tag="RH222_000094"
     CDS             complement(98666..98827)
                     /gene="rsmS"
                     /locus_tag="RH222_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV3795168.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(98841..99368)
                     /gene="priC"
                     /locus_tag="RH222_000095"
     CDS             complement(98841..99368)
                     /gene="priC"
                     /locus_tag="RH222_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV3795169.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            99438..99815
                     /locus_tag="RH222_000096"
     CDS             99438..99815
                     /locus_tag="RH222_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV3795170.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            99968..100519
                     /gene="apt"
                     /locus_tag="RH222_000097"
     CDS             99968..100519
                     /gene="apt"
                     /locus_tag="RH222_000097"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV3795171.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            100648..102579
                     /gene="dnaX"
                     /locus_tag="RH222_000098"
     CDS             100648..102579
                     /gene="dnaX"
                     /locus_tag="RH222_000098"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV3795172.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERNAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    101912..101976
                     /gene="dnaX"
                     /locus_tag="RH222_000098"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            102632..102961
                     /locus_tag="RH222_000099"
     CDS             102632..102961
                     /locus_tag="RH222_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV3795173.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            102961..103566
                     /gene="recR"
                     /locus_tag="RH222_000100"
     CDS             102961..103566
                     /gene="recR"
                     /locus_tag="RH222_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV3795174.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            103676..105550
                     /gene="htpG"
                     /locus_tag="RH222_000101"
     CDS             103676..105550
                     /gene="htpG"
                     /locus_tag="RH222_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV3795175.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            105731..106375
                     /gene="adk"
                     /locus_tag="RH222_000102"
     CDS             105731..106375
                     /gene="adk"
                     /locus_tag="RH222_000102"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV3795176.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            106611..107573
                     /gene="hemH"
                     /locus_tag="RH222_000103"
     CDS             106611..107573
                     /gene="hemH"
                     /locus_tag="RH222_000103"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV3795177.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(107570..108529)
                     /gene="aes"
                     /locus_tag="RH222_000104"
     CDS             complement(107570..108529)
                     /gene="aes"
                     /locus_tag="RH222_000104"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV3795178.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            108681..109985
                     /gene="gsk"
                     /locus_tag="RH222_000105"
     CDS             108681..109985
                     /gene="gsk"
                     /locus_tag="RH222_000105"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV3795179.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(110118..111794)
                     /locus_tag="RH222_000106"
     CDS             complement(110118..111794)
                     /locus_tag="RH222_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV3795180.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(112032..113252)
                     /gene="fsr"
                     /locus_tag="RH222_000107"
     CDS             complement(112032..113252)
                     /gene="fsr"
                     /locus_tag="RH222_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV3795181.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            113470..115122
                     /gene="ushA"
                     /locus_tag="RH222_000108"
     CDS             113470..115122
                     /gene="ushA"
                     /locus_tag="RH222_000108"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV3795182.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(115159..115638)
                     /gene="ybaK"
                     /locus_tag="RH222_000109"
     CDS             complement(115159..115638)
                     /gene="ybaK"
                     /locus_tag="RH222_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV3795183.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(115842..116636)
                     /locus_tag="RH222_000110"
     CDS             complement(115842..116636)
                     /locus_tag="RH222_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV3795184.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            116774..117115
                     /locus_tag="RH222_000111"
     CDS             116774..117115
                     /locus_tag="RH222_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV3795185.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(117330..119834)
                     /gene="copA"
                     /locus_tag="RH222_000112"
     CDS             complement(117330..119834)
                     /gene="copA"
                     /locus_tag="RH222_000112"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV3795186.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            120096..121028
                     /gene="glsA"
                     /locus_tag="RH222_000113"
     CDS             120096..121028
                     /gene="glsA"
                     /locus_tag="RH222_000113"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV3795187.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            121031..122323
                     /locus_tag="RH222_000114"
     CDS             121031..122323
                     /locus_tag="RH222_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3795188.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            122448..122855
                     /gene="cueR"
                     /locus_tag="RH222_000115"
     CDS             122448..122855
                     /gene="cueR"
                     /locus_tag="RH222_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV3795189.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(122856..123314)
                     /locus_tag="RH222_000116"
     CDS             complement(122856..123314)
                     /locus_tag="RH222_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV3795190.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(123311..124228)
                     /locus_tag="RH222_000117"
     CDS             complement(123311..124228)
                     /locus_tag="RH222_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV3795191.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            124374..125051
                     /gene="fetA"
                     /locus_tag="RH222_000118"
     CDS             124374..125051
                     /gene="fetA"
                     /locus_tag="RH222_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV3795192.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            125038..125817
                     /gene="fetB"
                     /locus_tag="RH222_000119"
     CDS             125038..125817
                     /gene="fetB"
                     /locus_tag="RH222_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV3795193.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(125880..126734)
                     /gene="cnoX"
                     /locus_tag="RH222_000120"
     CDS             complement(125880..126734)
                     /gene="cnoX"
                     /locus_tag="RH222_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV3795194.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(126795..127604)
                     /gene="ybbO"
                     /locus_tag="RH222_000121"
     CDS             complement(126795..127604)
                     /gene="ybbO"
                     /locus_tag="RH222_000121"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV3795195.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(127594..128217)
                     /gene="tesA"
                     /locus_tag="RH222_000122"
     CDS             complement(127594..128217)
                     /gene="tesA"
                     /locus_tag="RH222_000122"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV3795196.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            128188..128874
                     /locus_tag="RH222_000123"
     CDS             128188..128874
                     /locus_tag="RH222_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3795197.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            128871..131285
                     /locus_tag="RH222_000124"
     CDS             128871..131285
                     /locus_tag="RH222_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3795198.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFFSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(131997..133091)
                     /gene="mnmH"
                     /locus_tag="RH222_000125"
     CDS             complement(131997..133091)
                     /gene="mnmH"
                     /locus_tag="RH222_000125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV3795199.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEVGINYPLVEGGYKVLRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(133160..134086)
                     /gene="allS"
                     /locus_tag="RH222_000126"
     CDS             complement(133160..134086)
                     /gene="allS"
                     /locus_tag="RH222_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV3795200.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            134316..134798
                     /gene="allA"
                     /locus_tag="RH222_000127"
     CDS             134316..134798
                     /gene="allA"
                     /locus_tag="RH222_000127"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV3795201.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALRDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            134876..135691
                     /gene="allR"
                     /locus_tag="RH222_000128"
     CDS             134876..135691
                     /gene="allR"
                     /locus_tag="RH222_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV3795202.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            135781..137562
                     /gene="gcl"
                     /locus_tag="RH222_000129"
     CDS             135781..137562
                     /gene="gcl"
                     /locus_tag="RH222_000129"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV3795203.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            137575..138351
                     /gene="hyi"
                     /locus_tag="RH222_000130"
     CDS             137575..138351
                     /gene="hyi"
                     /locus_tag="RH222_000130"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV3795204.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            138451..139329
                     /gene="glxR"
                     /locus_tag="RH222_000131"
     CDS             138451..139329
                     /gene="glxR"
                     /locus_tag="RH222_000131"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV3795205.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            139498..140952
                     /locus_tag="RH222_000132"
     CDS             139498..140952
                     /locus_tag="RH222_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV3795206.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            141012..142373
                     /gene="allB"
                     /locus_tag="RH222_000133"
     CDS             141012..142373
                     /gene="allB"
                     /locus_tag="RH222_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV3795207.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            142430..143731
                     /locus_tag="RH222_000134"
     CDS             142430..143731
                     /locus_tag="RH222_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV3795208.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            143753..144898
                     /gene="glxK"
                     /locus_tag="RH222_000135"
     CDS             143753..144898
                     /gene="glxK"
                     /locus_tag="RH222_000135"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV3795209.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(145126..145911)
                     /gene="allE"
                     /locus_tag="RH222_000136"
     CDS             complement(145126..145911)
                     /gene="allE"
                     /locus_tag="RH222_000136"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV3795210.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(145922..>146719)
                     /locus_tag="RH222_000137"
     CDS             complement(145922..>146719)
                     /locus_tag="RH222_000137"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HDV3795211.1"
                     /translation="PDDVRNICDAKGNSFVDAMKACGFTLPNAPLTPRQDIKAFVELH
                     IEQGCVLESNGQSIGVVNAIVGQRRYTVTLNGESNHAGTTPMGYRRDTVYAFSRICHQ
                     SVEKAKRMGDPLVLTFGKVEPRPNTVNVVPGKTTFTIDCRHTDAAVLRDFTQQLENDM
                     RAICDEMDIGIDIDLWMDEEPVPMNKELVATLTELCESEKLNYRVMHSGAGHDAQIFA
                     PRVPTCMIFIPSINGISHNPAERTNITDLAEGVKTLALMLYQLAWQK"
BASE COUNT        35488 a        36775 c        38925 g        35531 t
ORIGIN      
        1 ttaggggaaa gaataattga accattcgtc tgtaacgcag cataatcgtt agcgcgaaac
       61 ataatatgtt ttctatgcat tgataattga tggatcaact tattacgtcc ctgaggaggg
      121 atgacaaatg cactcctgga aaaagaaact tgtagtatca caattagcat tggcttgcac
      181 tctggctatc acctctcagg ctaatgcagc aaactatgat acctgggggt atcatgacaa
      241 caccacgacc gatttagttt ggccgtatga caccgacaat gatggaattg ccgatgctgg
      301 cggcatggac ccgatgggtt cagatggtaa ctacgtaact tacaatggtt ttgtttacta
      361 taacaatgcc aatggcgatt tcgataccgt ctttaaaggc gataccgtta acggtaccat
      421 ttctacttat tacttgaatc atgattacgc tagcggttct gttaaccagg tagatatcag
      481 caattcagta attcatgggt caatcacttc tgaactgcca tttggttatt acagcattac
      541 gcctgaaggt tataatggct atactttcaa taacggaact actactgatc ttgactacaa
      601 ttggtacgat ggtgacgtat tcactctgaa cgtatccaat tccaccattg atgatgacta
      661 tgaagctctt tacttcactg acacacatct cgatggtgat aaatctaagt caaccaatga
      721 aacgttctat accgctctgg gtgttgcagt aaatcttgat gttgaaagca atatcaatat
      781 taccaacaac tcccgtgtag ctggcattgc gctgtctgaa ggcaatacaa gcaacacaac
      841 ttatactagc gaatatcatc agtgggacaa caacatcaat gtgtctaatt ccactgtaac
      901 ttcaggatca cagactccgc tggaagaaag tgatggcttc ttcggtaaat ctgctgaacc
      961 aagtgattac gctggtaatg gtggccagaa tgacgtagca ctgtcattca ctgacaatgc
     1021 tggctcaaat tattccatga aaaacaatgt aaactttgac cattcaactc tgttgggtga
     1081 tgtcgaattt acaagtcact ggaataacga tggcgtattc ttctattcca ccggtcatga
     1141 cagcaacggc gacggcgtcc ttgataccaa cggcggttgg gtagatgacg ctcagaacgt
     1201 tgacgaactg aatattactc tgaataacgg tagtaagtgg gttggttcag caaacatgag
     1261 tgctgaagta attgctccgg cagatatgta tgacgttgcg ccgaacagcc tgacccctgg
     1321 tgccaccatc gaagctaatg attggggccg tattattgac aacaaagtat tccagagcgg
     1381 cgtatttaac gtagcactga acaatagttc agaatggaat accgttaata gttcagtaat
     1441 tgatacatta gctgtaaata atggctctca ggtaaatgtt actgattcct ctctggtatc
     1501 agataccatt ggtttaacca acggttcttc actgaatatt ggtgcaaatg gcgtggttgc
     1561 tacagaccac ctgactgttg atagctacag taccgttaat ttaaccgaat ctaccggttg
     1621 gaataattat tctaatctgt ataccaatac tatcactgtg actaacggtg gtgtgctgga
     1681 tgtgaatgtt gatcagttcg atactgaagc tttccgtact gataaactgg aactgaccag
     1741 cggcaacatt gctgaccata atggcaacgt aatttctggt gtgttcgaca tcaacagcag
     1801 cgattacgtt ctgaacgctg atctggtgaa cgaccgtacg tgggatactt ccaagtctaa
     1861 ctacggttac ggtattgttg ctatgaactc tgatggtcac ctgactatca atggtaacgg
     1921 cgacgtagac aacggtactg aactggataa cagctctgtt gataacgttg ttgctgcaac
     1981 cggtaactac aaagttcgta tcgacaacgc aactggcgct ggcactatcg ctgattacaa
     2041 agataaagaa cttattcggg taaacgacgc taacaccaac gcgaccttct ctgctgctaa
     2101 caaagctgac ctgggtgcat acacctatca ggctgaacag cgcggtaaca ccgttgttct
     2161 gcaacagatg gagctgaccg actacgctaa catggcgctg agcatcccgt ctgcgaacac
     2221 caatatctgg aacctggaac aagacaccgt tggtactcgt ctgaccaact ctcgtcatgg
     2281 cctggctgat aacggcggcg catgggtaag ctacttcggt ggtaacttca acggcgacaa
     2341 cggcaccatc aactatgatc aggatgttaa cggcatcatg gtcggtgttg ataccaaagt
     2401 tgacggtaac aacgctaagt ggatcgtcgg tgcggctgca ggcttcgcta aaggtgacat
     2461 gaatgaccgt tctggtcagg tagatcaaga cagccagact gcctacatct actcttctgc
     2521 tcacttcgcg aacaacgtct ttgttgatgg tagcttgagc tactctcact tcaacaacga
     2581 cctgtctgca accatgagca acggtactta cgttgacggt agcaccaact ccgacgcttg
     2641 gggcttcggc ttgaaagccg gttacgactt caaactgggt gatgctggtt acgtgactcc
     2701 ttacggcagc gtttctggtc tgttccagtc tggtgatgac taccagctga gcaacgacat
     2761 gaaagttgac ggtcagtctt acgacagcat gcgttatgaa ctgggtgtag atgcaggtta
     2821 taccttcacc tacagcgaag atcaggctct gactccgtac ttcaaactgg cttacgtcta
     2881 cgacgactct aacaacgata acgatgtgaa cggtgattcc atcgataacg gtactgaagg
     2941 gtctgcggta cgtgttggtc tgggtactca gttcagcttc accaagaact ttagcgccta
     3001 taccgatgct aactacctcg gtggtggtga cgtagatcaa gactggtccg cgaacgtggg
     3061 tgttaaatat acctggtaat attcttcact ccgaagaaat tctggtaatt taatctaaat
     3121 aatgcccgtc aaggatttga cgggcattac tgcaaaggac gcgcaaatgt tatctgtagt
     3181 taaacctctt caggaatttg gtaagctcga taaatgtttg tccagatacg gtacgcgctt
     3241 cgagtttaat aatgaaaagc aagttatatt ttccagtgat gtcaataacg aagatacttt
     3301 cgttatttta gagggagtta tctctctgcg tagagaagaa aacgtactta tcggtattac
     3361 ccaggctcct tatattatgg ggctggctga tggtttaatg aaaaacgata taccatacaa
     3421 attaatatca gaaggaaatt gtacgggata tcatctacca gccaaacaaa ccattacgct
     3481 tattgaacaa aatcaactct ggcgagacgc tttttactgg ttagcctggc aaaatagaat
     3541 tctggaatta cgtgacgtgc agctcattgg gcataattcc tacgaacaaa tccgcgcaac
     3601 attattatca atgattgact ggaatgaaga attgcgatca cgtattggtg tgatgaatta
     3661 tatccatcaa cgtacacgca tatcgcgttc tgtcgtcgca gaagttctcg ctgctttgcg
     3721 taaaggcggc tatatcgaaa tgaataaagg caaactggtc gctatcaacc gtttgccttc
     3781 agagtattaa tcaggatgcg gggataacca actgtttatt cccgcttagc tcggtcacca
     3841 gatcattgat gccatcgctc atatttttaa agcgcgtcaa cggcgaacga gtgaccacca
     3901 caaacgcgcc aatattcttc tgcgggatca tcgccatata ggtaatgaaa ccgccaccac
     3961 cgcctgtctt ctgaataatc cccggacgac cctctttcgg ggccatatat acccagccta
     4021 aaccaagcgc atccgctttg ccggggacat ccatgccaat cactttggta aactgcgcgc
     4081 gctgataaat caacgtctgc atgcgatctg cctggttact gcgctgataa aaatcagatg
     4141 acaaatactg ctgcatccag cgcatcatat cgccaggcgt ggaatagacg ccgccgctgc
     4201 caattgccgc cagcgtgtta ttgcacggac tggcaccgcg ctcagcaacc attaaacggc
     4261 ggcactgatc cggtgagggg gtgtaggtgg tgtctttcat ccccaatgga cgggtaatct
     4321 gctcttcaaa caactgggta taaggcttgc cggacgcatt cgccagtgca tctgccagca
     4381 ggtcgaacgc aaggttagag tacgccgcct gactgcctgg cgcggctttc agcttcgccg
     4441 tagaaaggta tttccagcgt tgctcgcgcg ttggccagac aaacacggga cgatgtgccg
     4501 cgccaccggg ctgttcacgg ggcagggcgc tggtatgggt tgccagattg accagcgtaa
     4561 tgggcgtccc attgtaggtt ggcacgcgtg cgcctggcgg agcatattta cttagcggat
     4621 cgtttaactt cacggtcccc tggtcgagca atttcaccag catctcactg gtcattagct
     4681 tggtgaggga agcaatacgc acgaccgaat ccagctgcgg gcgaacgtta ttaccaggtc
     4741 gcgtttcgcc ataactgcga aagacgcgct ggttaccgtc gataacaacc agcgccatcc
     4801 ccgtggcacc gctgccgtaa aaaatatgat cggcataacg atcgacaata tcagaggcaa
     4861 actccggttc agtgatcggc tgcgccgcat ggacggaggt caatgacgcc gcacacagca
     4921 cggcagaaaa aagcagacta cgtttcaacg gtggtgtcca taaattgaat gaaggtaact
     4981 gagcgtattt atactactca ttgatggatt gcaaagggcg aattagtgca ataatagtgt
     5041 taaaaacgta acggcgcgta aacacgattt ttttcctgct caccaatgcc tgcatttgtg
     5101 atcctgcgcg tgttaatccg cttatgtaaa caatctttgg tttgtgaaac ccgtcgaaac
     5161 aattcttatg gtcaggctgg cgaactaagc gccttgctat gggtcacaat gggcggtttc
     5221 atttggctga cgctttgtag cggtcatgcg gttaatacac agcagttgtt aaaacgataa
     5281 gaagttagca ggagtgcata tgtttaagtc ttttttccca aagccgggaa cgttttttct
     5341 ctcggccttt gtttgggcat tgattgccgt tatcttctgg caagccggtg ggggtgactg
     5401 ggtggcgcgt atcaccggcg cttccgggca gatcccgatt agcgccgcgc gtttctggtc
     5461 gttggatttc ctgatttttt acgcttacta cattgtttgc gtaggacttt ttgcattgtt
     5521 ctggtttatc tacagcccgc atcgttggca atactggtca atactcggta ctgcgctgat
     5581 tatcttcgtc acctggtttt tggtggaagt cggggttgcc gtcaacgcct ggtatgcgcc
     5641 gttctatgat ctgattcaaa ccgcgctaag ttcgccgcat aaagtcacca tcgaacaatt
     5701 ttaccgcgaa gtgggcgtct ttctggggat tgcgctgatc gcggtagtga tcagtgtgct
     5761 gaataacttc tttgtcagtc actacgtgtt ccgctggcgt acggcgatga acgaatatta
     5821 catggcgaac tggcaacaac tgcgtcatat cgaaggtgcc gcacagcgtg tgcaggaaga
     5881 caccatgcgt tttgcttcaa cgctggagaa tatgggcgtc agttttatca acgccatcat
     5941 gacgttaatc gccttcctgc cggtactggt aacgctctcc gcgcatgtgc cggagctgcc
     6001 gattatcggg cacattccgt atggtctggt gattgccgct atcgtctggt cgctgatggg
     6061 gaccggattg ctggcagtgg tagggatcaa actgccgggg ctggagttta aaaaccagcg
     6121 tgtagaggct gcctaccgta aagagctggt ttatggtgaa gatgatgcca cgcgcgcgac
     6181 gccgcctacg gtacgcgagc tgtttagcgc cgtacggaaa aactattttc gcctctattt
     6241 tcactatatg tatttcaaca tcgcccgcat tctctatttg caggtcgata acgttttcgg
     6301 tttgttcttg ctgtttccgt caattgttgc cggtacgatt acgctcggcc tgatgacgca
     6361 gattaccaac gtttttggtc aggttcgcgg tgctttccag tacctgatta actcatggac
     6421 cacactggtt gagttgatgt ctatctacaa acgtctgcgc agctttgaac atgagctgga
     6481 tggtgacaaa attcaggaag taacccatac cttgagctaa aaaggagaag taatgtcgcg
     6541 cgtcaatcct ctatcttcgc tgtcattgtt ggcggttctg gttttagcgg ggtgtagtag
     6601 tcaggctccg caaccgttga aaaagggcga aaaagcgata gatgtaggga gcgtcgtgcg
     6661 gcagaagatg cctgcgagcg tgaaagaccg cgatgcctgg gcgaaagatc tcgccaccac
     6721 ctttgaaagt caggggctgg caccgacgct ggaaaacgtc tgctcggtgc tggcggtggc
     6781 gcagcaggag tcaaattatc aggcagatcc ggccgttccg ggcttaagca agatcgcctg
     6841 gcaagagatt gaccgtcgtg ctgaacggat gcacattcct ccttttctgg ttcataccgc
     6901 gctaaaaatc aaatcgccaa acggcaaaag ttatagcgaa cggttagatt cggtgcgtac
     6961 agaaaagcaa ttgagcgcga tttttgacga tctgatcagc atggtgccaa tggggcagac
     7021 gctgtttggt tcgcttaatc cggtgcgcac cggtgggccg atgcaggtca gcattgcttt
     7081 tgccgaacag cataccaaag ggtatccgtg gaaaatggac ggtacagttc gtcaggaagt
     7141 ctttagtcgc cgcggcgggt tgtggtttgg tacttaccat ttactgaatt atcccgccag
     7201 ttatagtgca ccgatatacc gttttgctga ttttaacgct ggctggtacg ccagccgtaa
     7261 tgccgcgttt cagaacgcgg tcagtaaggc cagcggcgtg aagctggcac tggacggtga
     7321 tttaattcgt tacgacagta aagaacccgg gaaaacagaa ctggcaacgc gcaaactggc
     7381 ggcaaaactg gggatgagcg acagcgaaat tcgtcgtcag ctagagaaag gtgacagctt
     7441 ctcttttgag gagacggcgc tgtacaagaa agtttatcaa cttgccgaag cgaaaaccgg
     7501 taaatcacta ccccgcgaaa tgttgcctgg cattcaactg gaaagcccga aaatcacccg
     7561 caacctgact acggcctggt ttgcgaagcg cgtagacgaa cggcgggcgc gttgtatgaa
     7621 acagtgatca aacaggaatg tcatgccaga taaggcgttt caggccgcat ctgacaacgt
     7681 aaaacttact ggcggcgatg tcgccagtgc agccatagcg ccaccactgc aaaaatgagg
     7741 cagcctacta aaaacgggat cagcccgaaa atggtgccga cacctaaacc aatttccacc
     7801 cgtgggcgac ctgtttcctg gacttgcatt aaacgctcat agacgccggg cgcatgcact
     7861 aacaggttca gcaattgcgc cccagcccaa aagcaaaaca gcacaaacaa cgcataggca
     7921 atgttgccag gcgttgaaga ggcattgcga tattttccgc taaacagcga ttttgacagg
     7981 gtgaaatcag ccataaaaac ctccggaagt catctgcttc tcttctggtg tgataccagc
     8041 ggcttatggc aagtctgaca ggtcatttag attgtcaata tcagaatcat ggtaaattga
     8101 tgttgggaat attcccgaag cgtgaatctt cgttgcgagt cacaaaacag caactttaag
     8161 ttaaatttat taacaattgc gaaagtgttg tctacaccac agacggttgt tacagaatat
     8221 gcaaggatgt tgtttttcgt taacggagct gccatgaatc tgcctgtaaa aatccgccgt
     8281 gactggcact actatgcgtt cgccattggc cttatattca ttcttaatgg cgtggtgggg
     8341 ttactgggat ttgaagcaaa aggttggcag acctatgccg tcggtctggt gacgtgggtg
     8401 attagtttct ggctggcggg gttgattatt cgtcgtcgcg atgaagaaac tgaaaacgcc
     8461 caataagaat attatcgggc gttaaaatat tacattgtgg ttttcaatgc gttatccgca
     8521 gcgtgacgct ccagcgccag ctcaatcaaa cgtgtgatca gatcggtgta acccagaccg
     8581 ctggcttgcc acagcttcgg atacatactg atgttggtga agccaggcag tgtgttgatc
     8641 tcgttgatca ccacttcgtt ctctggggtt aaaaacacgt ctacacgcgc catgcctgcg
     8701 catcccaacg tctgataagc ctgaacggca atcgcccgga tcttatcgtt gatctctggt
     8761 gcaatggctg ccggaactac cactttcgcg ccatcttcgt caatgtactt ggtgtcgtag
     8821 gcatagaaat cgctggtgag tacgatctcg ccacaggtgc tggcttgcgg attgtcgttg
     8881 cccagaactg cgcattcgat ctcacgacct ttgatccctt gctcaacgat cactttatga
     8941 tcgaactcga acgccagatc gacggcaatt gcgtactgtt cttcactggt tactttgctg
     9001 acaccaacag aagagccctg attagccggt tttacaaaca gtggtaaccc cagtttagac
     9061 tccacttcgg caaaactgat gttgtgacga ttagcgcgcg tcagggtaat aaatggcgca
     9121 atgttcagcc cggcatcgcg tagcagacgt ttggtgacat ctttatccat acaggctgct
     9181 gaagccagaa catcagaacc tacaaacggt aaattggcga cccgcagcat tccctgcaag
     9241 gaaccatctt cgcccagcgt accgtggaca atcgggaaaa tgacatccac cgtcggcaac
     9301 ggctgaccgt tttgcgcgtc gataagctga tgctcatgtt tacctggcac ctgcgcaagg
     9361 ctggtcgccg aagggcgcaa cgcaatatgg gcaggatcgt ctgcatttag cagataattg
     9421 ctggcatcgc tgacgtgcca ttgcccttgt ttatcaatgc ccagcagcac aacgtcgaag
     9481 cgacttttat caatggcatc gacaatgttt tttgccgatt gcagagacac ttcatgttcc
     9541 gctgatttac caccaaaaac gattcctacc cgcagttttt ccatcttaaa aacctatccc
     9601 gtctaacaca aagtgcatac attaccacga caaaacgggg gattcgcggc cttctgaaag
     9661 attgttgcaa tcttctgctg acaaagcgtg caacgtactg gtgaagaaag tgcgttatct
     9721 caaagatgtg cgcaagatca caaaaatgat gaacgggaag ctaatttatt cctggcttaa
     9781 atggcaatgc ggtgagtttt tttctcttaa ttataagtta acgaagagaa tatatttcat
     9841 aacttttatt tataataaag gttgataatt aaaagcctat attttgtgtg ggtagttatt
     9901 taaataagag aaacgtttcg ctggtaatca aacaaaaaat atttgcgcaa agtatttcct
     9961 ttgtcataaa aagaatactt ccagacacta tgaagttgtg aaacataatg ttaacttctc
    10021 catactttgg ataaggaaat acagacatga aaaatctcat tgctgagttg ttatttaagc
    10081 ttgcccaaaa agaagaagag tcgaaagaac tgtgtgcgca ggtagaagct ttggagatta
    10141 tcgtcactgc aatgcttcgc aatatggcgc aaaatgacca acagcggttg attgatcagg
    10201 tagagggggc gctgtacgag gtaaagcccg atgccagcat tcctgacgac gatacggagc
    10261 tgctgcgcga ttacgtaaag aagttattga agcatcctcg tcagtaaaaa gttaatcttt
    10321 tcaacagctg tcataaagtt gtcacggccg agacttatag tcgctttgtt tttatttttt
    10381 aatgtatttg tacatggaga aaataaagtg aaacaaagca ctattgcact ggcactctta
    10441 ccgttactgt ttacccctgt gacaaaagcc cggacaccag aaatgcctgt tctggaaaac
    10501 cgggctgctc agggcgatat tactgcaccc ggcggtgctc gccgcttaac gggtgatcag
    10561 accgccgctc tgcgtgattc tcttagcgat aaacctgcaa aaaatattat tttgctgatt
    10621 ggcgatggga tgggggattc ggaaattact gccgcacgta attatgccga aggtgcgggc
    10681 ggctttttta aaggtatcga tgcattaccg cttaccgggc aatacactca ctatgcgctg
    10741 aataaaaaaa ccggcaaacc ggactacgtc accgattcgg ctgcgtcagc aaccgcctgg
    10801 tcaaccggtg tcaaaaccta taacggcgcg ctgggcgtcg atattcacga aaaagatcac
    10861 ccaacgattc tggaaatggc aaaagccgca ggtctggcga ccggtaacgt ttctaccgca
    10921 gagttgcagg atgccacgcc cgctgcgctg gtggcgcatg tgacctcgcg caaatgctac
    10981 ggtcccagcg cgaccagtga aaaatgtccg ggtaacgcgc tagaaaaagg cggaaaagga
    11041 tcgattaccg aacagctgct taacgctcgt gccgatgtta cgcttggcgg cggcgcaaaa
    11101 acctttgctg aaacggcaac cgccggtgaa tggcagggaa aaacgctgcg tgaacaggca
    11161 caggcgcgtg gttatcagtt ggtgagcgat gctgcctcac tgaatgcggt gacggaagcg
    11221 aaccagcaaa aacccctgct aggactgttt gctgacggca atatgccagt gcgctggcaa
    11281 ggaccgaaag caacgtacca cggcaatatc gacaagcccg cagttacctg tacgcctaat
    11341 ccgcaacgta atgacagcgt accgaccctg gcgcagatga ctgataaagc cattgaattg
    11401 ttgagtaaaa atgagaaagg ctttttcctg caagttgaag gtgcatcaat cgataaacag
    11461 gatcacgctg cgaatccttg tgggcaaatt ggcgagacgg tcgatctcga cgaagccgta
    11521 caacgggcgc tggaattcgc taaaaaggat ggcaacacgc tggtcatagt caccgctgat
    11581 cacgcccacg ccagccagat tgttgcgccg gacaccaaag cgccgggcct cacccaggcg
    11641 ctaaatacca aagatggcgc agtgatggtg atgagttacg ggaactccga agaggattca
    11701 caagaacata ccggtagtca gctgcgtatt gcggcgtatg gcccacatgc cgccaatgtc
    11761 gttggactga ccgaccagac cgatctcttc tacaccatga aagccgccct ggggctgaaa
    11821 taaaaccgcg cccggcggtg aattttcgct gtcgggtggt ttttttgctg ttagcaacca
    11881 gacttaatgg cagatcacgg gcgcaaacgc tcatggttaa aacatgaaga gggatggtgc
    11941 tatgaaaata acattactgg ttaccttgct tttcggactg gtttttttaa ccaccgtcgg
    12001 cgctgccgag agaaccttaa ccccgcaaca acagcgtatg acctcctgta atcagcaggc
    12061 gacggcgcag gcgttgaaag gggatgctcg taagacctac atgagtgatt gcctgaagaa
    12121 cagcaagtct gcgcctggtg aaaaaagttt gacgccacag cagcaaaaga tgcgcgaatg
    12181 taataatcaa gcaacgcaac aatctctgaa aggtgatgat cgtaataagt ttatgagtgc
    12241 ctgcctcaag aaagccgcct gatacctgat agtgctaacg ggtgagctac gaaagtggct
    12301 cacccgaaat ttcatacttc tgcctttagc cccgtctcta taatttggga aaattgtttc
    12361 tgaatgttcc caaaaataat gaatgatgaa aactttttca aaaaagcggc ggcgcatggg
    12421 gaggaacctc ctttaactcc tcaaaacgaa catcagcggt ccgggctgcg cttcgcccgt
    12481 cgcgtcagac taccccgtgc ggttggcctg gctggcatgt tcttaccgat tgcttcaacg
    12541 ctggtttcgc acccgccgcc gggctggtgg tggctggtgt tggtcggctg ggcgttcgtc
    12601 tggccgcatt tagcctggca gatagcgagc agggccgtcg atccgcttag ccgggaaatt
    12661 tacaacttaa aaaccgatgc agtattagcg ggaatgtggg taggcgtaat gggcgtaaac
    12721 gtgctgcctt ccaccgcgat gttgatgatt atgtgtctga atttgatggg ggcaggcggc
    12781 ccccgtctgt ttgtcgcggg tctggtgttg atggtggttt cctgccttgt caccctcgaa
    12841 ctgacgggca tcaccgtgtc gttcaatagt gcgccgctgg aatggtggct ctcccttccc
    12901 attatcgtca tttatcctct gctgtttggc tgggtcagct accagacggc gactaaactg
    12961 gcggaacata aacgcaggtt gcaggtcatg agtacccgcg acggtatgac gggcgtgtat
    13021 aaccgacgtc attgggaaac tatgttacgc aatgaatttg ataactgtcg gcggcataat
    13081 cgcgatgcga cattactgat tatcgatatc gaccatttca agagcatcaa cgatacctgg
    13141 ggccatgatg tgggcgatga agcgattgtg gcgcttaccc gacagttaca aattaccctg
    13201 cgcggtagcg atgtgattgg tcggtttggc ggcgatgagt ttgcggtaat catgtccggt
    13261 acgccagctg agagcgccat taccgccatg ttacgggtgc atgaagggct aaacacgtta
    13321 cgcttgccga atacgccaca ggtaacttta cggattagtg tgggggttgc gccgctgaac
    13381 ccacaaatga gtcactatcg cgagtggttg aaatcggcag atttggcgct ttacaaagca
    13441 aagaaagccg gacgtaaccg caccgaagtg gcggcctgac gtccggcaaa aatcatcagg
    13501 atttgctgag tttttctgat ttttccatac acttcgtcat cgcttcgatc actgcggcac
    13561 ggaagccttt ctcttccagt acgcgtaccg cttcaatggt ggtgcctccc ggtgagcaga
    13621 ccatatcttt cagtgccccc ggatgttctc ccgtttccag caccattttt gcggaaccca
    13681 ttaccgcctg agcggcaaat ttatacgcct gggcgcgtgg catcccgccc agcacggcgg
    13741 cgtcggccat cgcttcgata aacataaata cgtaggctgg cgaagaaccg ctcacaccga
    13801 ccaccgggtg gatcatcggc tcagcaatta cttccgcttc gccaaagcag cggaaaatat
    13861 tcagcacatc agcggtatct tcgggggtta ccagcgcgtt tggcgttacg gaggtcatcc
    13921 cggcattaac cagcgcggga gtgttcggca tggcgcggat aattttccgg tcatggccca
    13981 gcgcgcgggc aagctggtcg agcgtgacac ctgcagcaat agaaacgacc agagagtctt
    14041 tattcaggct ggaggtgatt tcgctaagca ctttaatcat tatgccaggt ttaacggcag
    14101 caaaaatgat gtcggcgatt tgcgccactt cttgcgccga ttctgcggcg ttgatgccga
    14161 actggtcatg cagggcggcg actttatccg gggagggggt atatacccag atttgccctg
    14221 gaagcacctg accgctggca atcagaccgc cgagaatggc ttttcccata ttgccgcagc
    14281 caataaaacc gattttcttt tccattgcct cactcctgcc gtgaaattca ttgttttgat
    14341 aatcgctggc agaagcataa acagaactat gccggaaggc aaaagcgcga cacaatagag
    14401 gattacccaa caaaggatga ctttatgaca atttgggtgg atgccgacgc gtgtcccaat
    14461 gtaattaaag agattttgta tcgcgcggcg gaacgtatgc agatgccgct ggtactggta
    14521 gcaaaccaga gtttacgcgt gccgccatcg cgatttattc gtacgctgcg cgtcgcggca
    14581 ggtttcgacg ttgccgataa cgaaattgtc cggcagtgtg aagcgggcga tttggtgatc
    14641 accgcagata tacctttggc tgctgaagcc atcgagaaag gcgctgcggc gattaatccg
    14701 cgcggcgaac gttacacgcc agcgaccatt cgtgagcgcc tgacgatgcg cgattttatg
    14761 gataccttac gtgccagtgg gatccagacc ggcggaccag atagtctttc acaacgtgac
    14821 cgccaggcct ttgctgcaga gctggagaaa tggtggctgg aagtgcaacg tagtcgcggc
    14881 caaatgtaat ttattattta cacttcattc ttgattattt attggtatag taaggggtgt
    14941 attgagattt tcactttaag tggaattttt cctttacaat cgaaattgta ctagtttgat
    15001 ggtatgatcg ctattctcat gacaccggct ttcgccgcat tgcgacctat tggggaaaac
    15061 ccacgatgac acaacctctt tttctgatcg ggcctcgggg ctgtggtaaa acaacggtcg
    15121 gaatggccct tgccgattcg cttaaccgtc ggtttgtcga taccgatcag tggttgcaat
    15181 cacagctcaa tatgacggtc gcggagatcg tcgaaaggga agagtgggcg ggatttcgcg
    15241 ccagagaaac ggcggcgctg gaagcggtaa ctgcgccatc caccgttatc gctacaggcg
    15301 gcggcattat tctgacggaa tttaatcgtc acttcatgca aaataacggg atcgtggttt
    15361 atttgtgtgc gccagtatca gtcctggtta accgactgca agctgcaccg gaagaagatt
    15421 tacggccaac cttaacggga aaaccgctga gcgaagaagt tcaggaagtg ctggaagaac
    15481 gcgatgcgct atatcgcgaa gttgcgcata ttatcatcga cgcaacaaac gaacccagcc
    15541 aggtgatttc tgaaattcgt agcgccctgg cacagacgat caattgttga ttttcgagcg
    15601 cctatactta acgttcatcc cgtgaaataa ggaagaacga tgccaacgaa accgccttat
    15661 cctcgtgaag catatatagt gacgattgaa aaaggaaaac caggacagac ggtaacctgg
    15721 taccaactca gagccgatca tcctaaacca gactcgttga tcagtgaaca tccgaccgct
    15781 caggaagcga tggatgcgaa aaaacgctat gaggaccctg acaaagagtg atcgcatcag
    15841 actgctcgga agggattctg agtgccacta caagggatct gcgtcacatt tttcataatt
    15901 catgtttttc taataattag aatattaaac aataacaatc cattactgga atcatttgga
    15961 atctttacat tatgccgtgc acgtctgctg ctacgctttt tgtcatttgt agcacaagta
    16021 agtgtcagca gtggtgcttc acacttgccc ggtaattaac gacgaaagaa aagtaaggtg
    16081 gatgaacaat gagtgcgtcg ttggcgatcc tgaccatcgg cattgtacct atgcaggaag
    16141 ttttgccgct cctgacggaa tacattgacg aagataatat ttcccatcat agcctgctgg
    16201 ggaagttaag tcgtgaagaa gtgatggcgg agtacgcgcc agaagcaggc gaagacacca
    16261 ttctcacatt attaaatgac aaccagctgg cccatgtttc gcgtcgcaaa gtggagcgtg
    16321 acctgcaagg tgtggttgaa gtactcgata atcggggtta cgacgtcatt atattaatga
    16381 gtacggcaaa cattagtagt atgactgcgc gtaatacgat ctttcttgag ccgtcgcgga
    16441 tattgccacc gctggtttcc tctattgttg aagatcatca agtgggggtt atcgttccgg
    16501 ttgaggagat gctgcccgtt caggcgcaaa aatggcaaat tttgcagaaa tcgccggtat
    16561 tttcattggg taaccccatt catgattcag aacaaaaaat cattgatgcc gggaaagaat
    16621 tactggcaaa aggggctgat gtcatcatgc tggattgttt aggatttcac caacgtcatc
    16681 gcgatttact gcaaaaacag ctcgatgttc ctgtcttgct gtctaacgta ttgattgcac
    16741 ggctggctgc ggaattactg gtgtaatttt gcgtgacagt cagcgcctct ggcccctata
    16801 gtgaagtaga tattcaacta ccagacaggg ccagtttatg cttcaaagta atgagtactt
    16861 ttccggcaaa gtgaaatcga tcggcttttc cagcagcagc actggtcgcg ccagtgtggg
    16921 tgttatggtt gaaggcgaat acaccttcag caccgctgag ccggaagaga tgacggtaat
    16981 cagtggcgcg ctgaatgtgt tactgcctga cgcgaccgac tggcaagtgt atgaagccgg
    17041 ttcggtgttt aatgttcccg gtcacagcga gtttcatctg caagttgccg aacccacctc
    17101 ttatctgtgt cgctatctgt aattcctcgc cttccccttg aacgggaggg catttttctg
    17161 aaatatcctc tcataaaccc ataataatat ttcctttgct gcgatttttt cgatttccgg
    17221 tatattcata attcatcaag gttgatataa atatcagtga agatctccat gtattttttg
    17281 cggtactggc tacgataaaa gataaatcag atgatgaatg gtggcgtgca ttgctgcaaa
    17341 gtcgcaatga tcatttgcgc caaacggcat tgcgcaacgc acataccccg gcgttgttgt
    17401 taacgacatt gactgagcct caggatcggt cgctggctat taataatcca cagctgtctg
    17461 ccgatgtgaa aacggcgtgg ttaaaagagg atccatcatt actcttattt gtcgaacaac
    17521 ccgatctttc gcagttacgt gatttagtga aaaccggggc aacgcggaaa attcgcagtg
    17581 aagcgcgtca ccggcttgag gaaaaacaat gacacaacgt ccatggagca agttacagcg
    17641 taaaaagcat aatattgctg cgctaaaaat tattgctcgc cgtagcgaat aattatatgc
    17701 ctggtgtggc ttcgtacgcc ggataagacg cggcaggcgt cgcatccggc attaaaggaa
    17761 aatcagcaat taacgttgtg cttcgccacc taatccttca atcaggtttt gaattaacgc
    17821 tgccagttca ccagtcatca ggatgaaatc ggcatcaaaa cgctgggcga aatcttcacg
    17881 gtcgatatct tcgttttgat cgcgcagctc gtcgcagaac ttcagacgct tgagcgaacc
    17941 atcgtcgcac atcacaaact gaatgcgctg ctgccagtcg agcgccagtt tagtcaccac
    18001 ttttccggct tcaatgtgat tggtgatctc ttcgctggtc agatcttgtt tcttcgcgcg
    18061 gatcacgccg ccatcttcca gcaacgattt cagctcggct tcatcaagca gctggaagcc
    18121 ctgtgccgca ctaccggagc gaacccattc ggtcagcgtc agttcaatcg ggttttccat
    18181 gctcaatggt acaaccggta acgaccccag gcttttacgc agtaatgcca gcgtatcttc
    18241 cgcttttttg gcactggcgc agtccaccat aatcaaacca ttaaccgtgt cgatccacat
    18301 cattgtctgg ctaaaacggc tgaaagcacg cggcaacaga gagtgcagca cttcgtcttt
    18361 cagcgaatct ttttcggttt tcttcagctt acgcgcctgt tccgcttcca gtttggcgat
    18421 tttcgcttcc agcgcctgtt taatcaccgg ggacgggagg attttttctt ctttgcgcgc
    18481 gcagataaca atttgaccat tggcaacgtg cgttaacgca tcgctgtgcg atcccatcgg
    18541 aggaacccag cccatcttcg ccatgtcctg gctgccgcat ggggtaaatg ccatcgaggc
    18601 tagctgtttt tccatctctt ctgcacgcag cgaaatctcg cggctaagac ggtaaaccat
    18661 taaatttttg aaccacagca tgataatttc cacggccttg tcgttaaatt tagcgggcat
    18721 gataacgaat tgtcggcggc cttgcattgc caatccggtt gtccgtctct acgctattga
    18781 tattgaaaaa aataaggaga gtaccgtgcg tataggtatc gatttaggcg gcaccaaaac
    18841 tgaagtgatt gcactgggcg atgcagggga gcagttgtac cgccatcgtc tgcccacgcc
    18901 gcgtgatgat taccggcaga ctattgaaac gatcgccacg ttggttgata tggcggagca
    18961 ggcgacaggg cagcgcggaa cggtaggtat gggcattcct ggctcaattt cgccttacac
    19021 cggtgtggtt aagaatgcca attcaacctg gctcaacggt cagccattcg ataaagactt
    19081 aagcgcgagg ttgcagcggg aagtgcggct ggcaaatgac gccaactgtc tggcggtttc
    19141 agaagcagta gatggcgcgg cagcgggagc gcagacagta tttgccgtga ttatcggcac
    19201 gggatgcggc gcgggcgtgg cattcaatgg gcgggcgcat atcggcggca atggcacggc
    19261 aggtgagtgg ggacacaatc cgctaccgtg gatggacgaa gacgaactgc gttatcgcga
    19321 ggaagtccct tgttattgcg gtaaacaagg ttgtattgaa acctttattt cgggcacggg
    19381 attcgcgacg gattatcgtc gtttgagcgg acatgcgctg aaaggcagtg aaattatccg
    19441 cctggttgaa gaaagcgatc cggtagcgga actggcattg cgtcgctacg agctgcggct
    19501 ggcaaaatcg ctggcacatg tcgtgaatat tctcgatccg gatgtgattg tcctgggggg
    19561 cgggatgagc aatgtagacc gtttatatca aacggttggg cagttgatta aacaatttgt
    19621 cttcggcggc gaatgtgaaa cgccggtgcg taaggcgaag cacggtgatt ccagcggcgt
    19681 acgcggcgct gcgtggttat ggccacaaga gtaaaaaacg taggcaattg gcgcatcatg
    19741 cttgatgcga cgcttgccgc gtcttatcag gcctacaaaa tgtgccggaa ccgcgtaggg
    19801 cggataaggc gttcacgccg catccggcaa taagcaacgc gcaacgtgac tgcaatctac
    19861 cccagtggtt tcgccagcac cggactatcc gccgcttgct ggcgcttata gcgaccatac
    19921 agcagcaatg acgacatcgc agcaaacgaa agcagggcgg caggcagcgc cacgtaatta
    19981 tatgccagcc ccagcgtcag catcatacca ccgcaatatg cgccgacggc gctaccaagg
    20041 ttaaacgcta tttgcccacc tgcggcacct aataactctc cgcctttggc attttgtagt
    20101 aataatattt gcagaggtgc tgaaagggca aataaccccg cgcaacaaat aaaagcaaaa
    20161 ataagcgacg ttgttttcat gccgccgcag aaaaagagca tcagcagtgc cagtacaatt
    20221 ataaagtcag tcactgctgc aatgcgcagt ggtgaataac gtcctgaaat cctgccactt
    20281 agcatatttc ccaacaccat ccctagccca actaacatca taataaaggt catcgccgtt
    20341 tccgaaaaac cggaaataaa catcatgtat ggctttacgt agctgaacca ggcaaacaca
    20401 cctgcgttgc caaacatcgt ggcggcgaaa attaaccacg gggccgggct gcgtaaaaag
    20461 tgaaattgtt cgcgcagctt tcctttcgcc tcgtcgcgaa tatccggcac ccaaaaatag
    20521 accgatgcca tcaccgcaat attaaaaaca gcgatcaata aaaaggtgta acgccagcta
    20581 aattcctgac ttaaatacgt tcccagcgga atgcccagca aattggcgac tgtcatcccg
    20641 gaaaccatcc ccgccacggc ggcggtgact tttccgggtt tgataatttt tgataacacg
    20701 atcgcgccga cgccaaaaaa tgcgccatgc ggaaagccgg aaaccaggcg accaatggca
    20761 agcatcatgt aagacgaaga gagcgtgaac atggcgttgc caatgacgca caacgccacc
    20821 agaaacaaca agatatgttt gagtgagtag cggctggaaa agagtgcgat gattggcgca
    20881 ccgaccacca ctcccagcgc ataatacgag atcatatgcc cggcagcagg aatcgaaatt
    20941 cctacgttat gcgccagctc cgtgagcacg cccataatgc caaattcggc catccccaaa
    21001 ccaaacgtgc ccagagccaa agataaaatg acttttttca taccaccatc acaactacca
    21061 gaacggctac caatcaccgt agccaataaa gtgatagctt gcaggataac gaccagcaag
    21121 gccaagagat aattcgcccc tctgtattca ttatcctgct gaatagttat ttcactgcaa
    21181 acgtactttc cagtttgctg tagcccaggc cgttgatctt tttcactttg atctgcaccg
    21241 gaatacgctc tttcatcgct tctacgtggc taatcacacc gatggttttg ccactggcgt
    21301 tcagggcatc cagcgcatca agggcggtat ccagcgtttc gctatccagc gtgccaaaac
    21361 cttcatcaag gaacagcgag tcaatacgtg ttttatggct gaccagatcc gaaagcgcca
    21421 gcgccagcgc cagactaacg aggaaacttt cgccgccgga aagggtacgg gtatcgcgta
    21481 ccgcatctgc ctgccaggta tcaacaacct cgacttccag cgcctcgctg gctttgcgct
    21541 gtaacagata gcgcccgtgc agccgggtaa gttgctgatt agcgagatgg actaaattat
    21601 ccagcgtcag cccctgggca aacttgcgga atttatcgcc ctctttggaa cctattagcg
    21661 aattcagata tccccagtcc tcaacctgct gcgtcatttg agcaatttgc tgcattaagg
    21721 tttgttgttg ctgacggtta tctgcatcct gcttcagctg ctggcgaatc tcgccttgac
    21781 tcgtggtgtt ttcacgcaac ttttggtgag tttgcgctaa ctcttgctga atctgctcca
    21841 ccgtcacagt gagagccaac ccgtcaggtc ggtgttgttg atgctgtgcc agcgtttctg
    21901 ctgtctgagt gaccagcgtt tgcgcctgac ggcgctggtt ttccagattc tgcttgagct
    21961 gttccagctg cgttagtgtt tgttcatcca ttagcgccgc aaggaacgcc tgctgatcgt
    22021 caaagacgct ggcctgtagc gcggtgtcaa actgcgcctg ggctttttgc agactttgcg
    22081 ccgccagaac gtcctgttgc tgtaacgtct gctgctggct gtgtaatgcg agacattgtt
    22141 catgaacctg ccgccagtta tccagcgcca cagtttcttc gctgtgcggg agatcatcac
    22201 tttgcggcaa cgtttccaga atcggcgtca gctgctgaat acggttttgc agcgcggtta
    22261 attcgttctg gcgttgctgc cagctctgcg cttcttgctg acgtgtcgcc aaccagctct
    22321 cttcttcatc ttcctgtggc aatgtcagtg cataacccgc caatgccgtt aagagctgct
    22381 gctggcgttg ttcaatttgc tgttgatact ggataatttg ctgattatgc gcggcaatct
    22441 gcccttgtaa ttcatgtcgt tggctgagta accgcagctg gcgttcgtgc tcatcttgtg
    22501 catccagcca cggttgaata tcgtcctgtg gctgcaaggt gatattgagg ctggccgtga
    22561 cggcttgcca ttgttgagta agtgcttgct catcttgtcg gaggctttgc gcttcgtttt
    22621 catcgcgctg aagctgcttc gttaatgcat ccagttgccc gcgcagcgcc gcaccttctt
    22681 cgccgagctt tttaacttcg ttttccagcg ccagtaatcg agactgatta acaccaggct
    22741 ccagcgcctg atacgcctcg accgccgggt ggctggtgga accacaaagt ggacaaggct
    22801 gacccgcctg taactgtgca cgttgagctt ccagcgtttt gatgcgcgct tcctgctcgc
    22861 aaatggtttt cacatcggca agttgctgcg tcttttcttt ataacgctgg cgcatttcgt
    22921 taagtgcggc gttacgttgc gtctgttctt gcgtgacatt ctggatagcg acctgtaact
    22981 gcgccagacg tttttgttgg ggaacaatct gcccatgcag cgcgaccagg cgctgacgca
    23041 gtgggcgttg ctcggcatgt tgcgccaggg cgctagcaac ttcatcggcg gttaacgtca
    23101 acgtgatcgc cgcaagcgta ttaagttttt gctcagcatg ggttaactgt tgctgccatt
    23161 gccgcagatg ctcgcgatcg ctggtttgtt gggagaactg cgcacgccaa cccgccagtt
    23221 cgttgttcca ctgacggaag cggtcgtgtt cctgtaacca ggtattcagg ctttgttgct
    23281 gctgctgtaa ttctgctgac tgcttcgccg cgtggtggcg aatgctcgcg cgaagcgcca
    23341 ttgtgctctg taagcgagta tttacttctt caatctgctg gcgaatatgc gccagcgccg
    23401 cgctgtgttc tgcgatgcgt tcccagtgtg gacgaagatt tcgtgccggt tgtgccagac
    23461 taagcgccgc cagttgaggt tgcgcttttt cttcttcggc taacacctgt tgcaaggcct
    23521 gctgacggcg gctggcttct tgctgcaatt cgtcctgacg cgttaaccag tttagcgatt
    23581 gttgttcttg ctgctgcgcg gtaagtaact gtttttcttc gtcagtaagt acctgcaaac
    23641 tcgctgtcag cgattgcact tgttccggcg tgagcaacgc gacgccgctg gcctgcgctt
    23701 gcagcttctc cagctctgtg cgggccgatt tgtgctgctc aaaaaccatc gccgagattt
    23761 gcccgtagat ttcagtgccg gttaactcct cgagcaattc cgcgcgttct ttgggtttgg
    23821 cattcaggaa ggcagcaaat tgcccctgcg aaagcagcat cgaacgggtg aagcgcccgt
    23881 aatccagccc ggttaacgtc gctgtcagtt ccagcttatc tttcactttg tcggcgagaa
    23941 ttttgccgtc ggcgcagcgc gccagctcta cgcgtggcac ctgcaaatta ccgtcgggtt
    24001 ggttacgcgc ccgattctgg ctccagaatg cacggtacgc ttcacctttc acttcaaact
    24061 ccacctccgc cagacattca gcggtatcgc gggtcatgag atcattttgc gattgtgaaa
    24121 cgttagagag acgcggagtt tcatgataca gcgccagaca aatggcgtcc agcagggtgg
    24181 ttttccccgc accggttggg ccggtaatgg caaacagccc gttgctggcg aacggctcgc
    24241 gggtgaaatc aatcttccat tcgcctttta atgagttcag gtttttcagg cgcaggctga
    24301 gaattttcat gcttcgtgtt ctccggcgag ggtatgcaac gtcgtggtga aaagatgctg
    24361 cagacgttgc tgctgcgatt catccagttc ttccagtgcc agacggcgat tgaacacctc
    24421 ttcgacgctg agttcgctga gggtttcacg ctgttggctg gctaacacac gctcgcgctg
    24481 ttcacgactc cggcgtacca gcaatacttc aacaggcaat gattcggtta atgcctggat
    24541 tttgcgctga atatcatgca gatactcatc agtagtgatt tcgatatcca gccagacagg
    24601 cggctcctgt gatacatcgc gccactgttc cagctgtgcg gtaatcgacg ccagatcgcc
    24661 tttcagcact gccatgggtt gcgttaccgg tacgttcagg ttttccacgc tctctaattt
    24721 gccgtttgaa aatgtcacca gatggacata tttactctta ccgcattcat caaaactcag
    24781 tggaatgggg gagccgcaat agcgaacatg ttccatgccg ccaataatct gtgcgcggtg
    24841 aatatgcccg agcgcgatgt agtcggctgg tggaaagttt tgtgccggaa acgcgtccag
    24901 cgtgccaata taaatgtcac gcacggcgtc acttttactg gcccccacgg tcgttaaatg
    24961 tcccgtggcg atgatgggca gaggctgatc gccgcgcagt ttgcaggcat cggcatagtg
    25021 ttgttggtaa taatcggtaa tcgctgccag taaatgctgc tgtttttcaa taccgttaag
    25081 ccccgcctgg ctggtaataa tgtcacgtgg acgtaaaaac ggaatggggc acagcactgc
    25141 gcctggcgtc ccgtcgcgac gaggcaagat ttgcggcgca tgtccggcgc tggcgaccac
    25201 ggtagtattg aggaacgcca tgatatcgcg cgattcattc agcgtggcga ccgagtcatg
    25261 gtttcctgcc agtaccacca gatgacagcc agtttgctgt aaattgacaa caaaacggtt
    25321 gtataacgtg cgggcgtaac tgggcggcga gccggtatcg aaaacatcac cagcaacaat
    25381 aatcgcatcc acctgatggg tttgtgctgt ctccagcagc cagtcaagaa aagcctgatg
    25441 ttcagcttcg cggcttttac tgtagaagtt ctggccgaga tgccagtctg aggtgtgaag
    25501 gatgcgcata acggttccct ggcgaaaaag catgggcgcg attataccca aacagatgtg
    25561 ccatttgctt ttttctgcgc cacggaaatc aataacctga agatatgtgc gacgagcttt
    25621 tcataaatct gtcataaatc tgacgcataa tgacgtcgca ttaatgatcg caacctattt
    25681 attacaacag ggcaaatcat ggcgagacgt attctggtcg tagaagatga agctccaatt
    25741 cgcgaaatgg tctgcttcgt gctcgaacaa aatggctttc agccggtcga agcggaagat
    25801 tatgacagtg ctgtgaatca actgaatgaa ccctggccgg atttaattct ccttgactgg
    25861 atgttacctg gcggctccgg tatccagttc atcaaacacc tcaagcgcga gtcgatgacc
    25921 cgggatattc cagtggtgat gttgaccgcc agaggggaag aagaagatcg cgtgcgcggc
    25981 cttgaaaccg gcgcggatga ctatatcacc aagccgtttt cgccgaagga gctggtggcg
    26041 cgaatcaaag cggtaatgcg ccgtatttcg ccaatggcgg tggaagaggt gattgagatg
    26101 cagggattaa gtctcgaccc gacatctcac cgagtgatgg cgggcgaaga gccgctggag
    26161 atggggccga cagaatttaa actgctgcac ttttttatga cgcatcctga gcgcgtgtac
    26221 agccgcgagc agctgttaaa ccacgtctgg ggaactaacg tgtatgtgga agaccgcacg
    26281 gtcgatgtcc acattcgtcg cctgcgtaaa gcactggagc ccggcgggca tgaccgcatg
    26341 gtgcagaccg tgcgcggtac aggatatcgt ttttcaaccc gcttttaacg ccttgctcat
    26401 cggacgcaga gcagggctta tgatttctta actggagtat cttacgtgct ggaacggctg
    26461 tcgtggaaaa ggctggtgct ggagctgcta ctttgctgcc tcccggcttt catcctgggt
    26521 gcattttttg gttacctgcc ctggtttttg ctggcatcgg taacaggact gcttatctgg
    26581 catttctgga atttattgcg cctttcatgg tggctgtggg tggatcgcag tatgaccccg
    26641 ccaccggggc gtggtagctg ggaaccgcta ctatacggct tacaccagat gcagctgcga
    26701 aataaaaaac gccgccgtga actgggcaat ctgattaaac gctttcgtag cggcgcggag
    26761 tcgctgcccg acgcggtggt gctgaccacg gaagagggcg gtattttctg gtgtaacggt
    26821 ctggcgcaac aaattcttgg tttgcgctgg ccggaagata acgggcagaa catccttaac
    26881 ctactgcgtt acccggagtt tacgcaatat ttgaaaacgc gtgatttttc tcgcccgctc
    26941 aatctggtgc tcaacaccgg gcggcatctg gaaattcgcg tcatgcctta tacccacaaa
    27001 cagttgctga tggtggcgcg tgatgtcacg caaatgcatc aactggaagg ggcgcggcgt
    27061 aacttttttg ccaacgtgag ccatgagtta cgtacgccat tgaccgtgtt acagggttac
    27121 ctggagatga tggatgagca gccgctggga ggcgcggtac gcgaaaaagc gttgcacacc
    27181 atgcgcgagc agacccagcg gatggaaggg ctggtgaagc agttgctaac gctgtcgaaa
    27241 attgaagccg caccgacgca attgctcaat gaaaaggttg atgtgccgat gatgctgcgc
    27301 gttgttgagc gcgaggctca gactctgagt cagaaaaaac agacatttac ctttgagata
    27361 gataacggcc tcaaggtgtc tggcaacgaa gatcagctac gcagtgcgat ttcgaacctg
    27421 gtctataacg ccgtgaatca tacgccggaa ggcacgcata tcaccgtacg ctggcagcga
    27481 gtgccgcacg gtgccgaatt tagcgttgaa gataacggac cgggcattgc accggagcat
    27541 attccgcgcc tgaccgagcg tttttatcgc gttgataaag cgcgttcccg gcaaaccggc
    27601 ggtagcggat tagggttagc gatcgtgaaa catgcggtga atcatcacga aagtcgcctg
    27661 aatattgaga gtacagtagg aaaaggaaca cgtttcagtt ttgttatccc ggaacgttta
    27721 attgccaaaa acagcgatta atccgccttt gtcatctttt attgccataa gccagccgat
    27781 gctggcttat tttctttgca gtcaaaatac gggcgttaga ttttacaacg gttggtgatt
    27841 ttttgttcgc atgattagcc atgtcttttt cacggaaata gtgttttata ctggttggtg
    27901 atttcttatc gctatatacc tctggttttt agatccctcc ttgctttaaa acgttataag
    27961 cgtttaaatt gcgcttcagg tgctgtcata ctgactgcat taacgcggta aatcgaaaaa
    28021 ctattcttcg ccgcgcctgg ttgggagtat ttcccgctaa aattgtttaa atataccgct
    28081 gtatcatccc cagggattgg cacaaaaatt taacgttaca acaccacatc cacaggcagt
    28141 atgatttatg acccatcaat taagatcgcg cgatatcatc gctctgggct ttatgacatt
    28201 tgcgttgttc gtcggcgcag gtaacattat tttccctcca atggtcggct tacaggcagg
    28261 cgaacacgtc tggactgcgg cattcggctt cctcattact gccgttggcc tgccggtatt
    28321 aacggtagtg gcgctggcaa aagttggcgg cggtgttgac agcctcagca cgccaatcgg
    28381 taaagtcgct ggcgtactgc tggcaacggt ttgttacctg gcggtggggc cgcttttcgc
    28441 tacgccgcgt acagctaccg tttcttttga agtggggatt gcgccgctga cgggtgattc
    28501 cgcgctgccg ctgtttatct acagcctggt ctatttcgct atcgttattc tggtttcgct
    28561 ctatccgggc aagctgctgg ataccgtggg caacttcctt gcgccgctga aaattatcgc
    28621 gctggtcatc ctgtctgttg ccgcgattgt ctggccggcg ggttctatca gcacggcgac
    28681 tgaggcttat caaaacgctg cgttttctaa cggcttcgtc aacggctatc tgaccatgga
    28741 tacgctgggc gcaatggtgt ttggtatcgt tattgttaac gcggcgcgtt ctcgtggcgt
    28801 taccgaagcg cgcctactga cccgttatac cgtctgggct ggcctgatgg cgggtgttgg
    28861 tctgactctg ctgtacctgg cgctgttccg tctgggttca gacagcgcgt cgctggtcga
    28921 tcagtctgca aacggcgctg ctattctgca tgcttacgtt cagcacacct ttggcggcgg
    28981 cggtagcttc ctgctggcgg cgttaatctt catcgcctgc ctggtaacgg cagttggcct
    29041 gacctgtgct tgtgcagaat tctttgccca gtacgtaccg ctctcttatc gtacgctggt
    29101 gtttatcctc ggcggcttct cgatggtggt ttctaacctc ggcttaagcc agctgatcca
    29161 gatctccgta ccggtgctga ccgctattta tccgccgtgt atcgcactgg ttgtattaag
    29221 ttttacacgc tcatggtggc ataattcgtc ccgcgtgatt gctccgccga tgtttatcag
    29281 cctgcttttt ggtattctcg acgggatcaa agcatctgca ttcagcgata tcttaccgtc
    29341 ctgggcgcag cgtttaccgc tggccgaaca aggtctggcg tggttaatgc caacagtggt
    29401 gatggtggtt ctggccatta tctgggatcg cgcggcaggt cgtcaggtga cctccagcgc
    29461 tcactaaatc actgaacatt tgttttaacc acggggctgc gatgccccgt ggttttttat
    29521 tgtgttgatg ggttaggaat tgatggaaag taagaacaag ctaaagcgtg ggctaagtac
    29581 ccgccacata cgctttatgg cactgggttc agcaattggc accgggctgt tttacggttc
    29641 ggcagacgcc atcaaaatgg ccggtccgag cgtgttgttg gcctatatta tcggtggtat
    29701 cgcggcgtat atcattatgc gtgcgctggg ggaaatgtcg gtacataacc cggccgccag
    29761 ctctttctcg cgttatgcgc aggaaaacct cggcccgctg gcaggttaca ttaccggctg
    29821 gacctactgc tttgaaatcc ttattgtcgc catcgccgat gtgaccgctt ttggtatcta
    29881 tatgggtgtc tggttcccga cggtgccgca ctggatttgg gtactgagcg tggtgctgat
    29941 catttgcgcc gtaaacctga tgagcgtgaa ggtattcggt gagctggaat tctggttctc
    30001 gttctttaaa gtcgccacca tcatcatcat gattgtcgcc ggtttcggca tcatcatctg
    30061 ggggattggc aacggcgggc aaccgaccgg tattcataac ctgtggagca acggcggctt
    30121 cttcagtaac ggctggcttg gcatggtaat gtcgttgcaa atggtgatgt ttgcttacgg
    30181 tgggatcgaa attatcggga ttaccgccgg tgaagcgaaa gatcctgaga aatcgatacc
    30241 gcgtgcgatt aactccgtgc cgatgcgtat tctggtgttc tacgtcggta cgctgttcgt
    30301 cattatgtct atctacccgt ggaatcaggt tggcactgcc ggtagcccgt tcgtgctgac
    30361 gttccagcat atgggcatta cctttgccgc cagcattctt aactttgttg tgctgactgc
    30421 ttcgctgtcg gcaattaaca gtgatgtatt tggcgtaggc cgtatgctcc acggtatggc
    30481 agagcagggc agcgcgccga aaattttcag caaaacgtcg cgtcgcggta ttccgtgggt
    30541 tacggtgctg gtgatgacta ccgcgctgct gtttgcggtg tatctgaact acatcatgcc
    30601 ggaaaacgtc ttcctggtga tcgcttcgct ggcaaccttc gccacggtgt gggtgtggat
    30661 tatgatcctg ctgtcgcaaa ttgccttccg tcgccgtttg ccgccagaag aagttaaggc
    30721 gctgaaattt aaagtgccgg gtggggtagc aacgaccatc ggcgggctga ttttcctgct
    30781 ctttattatc gggttgattg gttatcaccc ggatacgcgt atctcgctgt atgtcggttt
    30841 cgcgtggatt gttgtgctgt tgattggctg gatgtttaaa cgccgccacg atcgtcagct
    30901 ggctgaaaac caataatact tgcgttctgt aagccggttc aggaggaaat cctgatccgg
    30961 ctattccgaa agttatccgc ccccgtcctc ctcccccaaa tatccttcag atgatgagtg
    31021 atcctgcatt aggctatggc aaggtgatca gattttcatc acaggggaat tatgatgtta
    31081 aatgcatggc acctgccggt gcccccattt gttaaacaaa gcaaagatca actgctcatt
    31141 acactgtggc tgacgggcga agacccaccg cagcgcatta tgctgcgtac agaacacgat
    31201 aacgaagaaa tgtcagtatc gatgcataag cagcgcagtc agcagcagcc tggcgtcacc
    31261 gcatggcgtg cggcgattga tctctccagc ggacaacccc ggcggcgtta cagtttcaaa
    31321 ctgctgtggc acgatcgcca gcgttggttt acaccgcagg gcttcagccg aatgccgccg
    31381 gcacgactgg agcagtttgc cgtcgatgta ccggatatcg gcccacaatg ggctgcggat
    31441 cagatttttt atcagatctt ccctgatcgt tttgcgcgta gtcttcctcg tgaagctgaa
    31501 caggatcatg tctattacca tcatgcagcc ggacaagaga tcatcttgcg tgactgggat
    31561 gaaccggtca cggcgcaggc gggcggatca acgttctatg gcggcgatct ggacgggata
    31621 agcgaaaaac tgccgtatct gaaaaagctt ggcgtgacgg cgctgtatct caatccggtg
    31681 tttaaagctc ccagcgtaca taaatacgat accgaggatt atcgccatgt cgatccgcag
    31741 tttggcggtg atggggcgtt gctgcgctta cgccacaata cgcagcagct gggaatgcgg
    31801 ctggtgctgg atggcgtgtt taaccacagt ggcgattccc atgcctggtt tgacaggcac
    31861 aatcgtggca cggggggagc ttgtcacaac cctgaatcgc cctggcgcga ctggtactcg
    31921 tttagtgatg atggcacggc gctcgactgg cttggctatg ccagcttgcc gaagctggat
    31981 tatcagtcgg aaagtctggt gaatgaaatt tatcgcgggg aagacagtat tgtccgccat
    32041 tggctgaaag cgccgtggag tatggacggc tggcggctgg atgtggtgca tatgctgggg
    32101 gaggcgggtg gggcgcgcaa taatatgcag cacgttgctg ggatcaccga agcggcgaaa
    32161 gaaacccagc cggaagcgta tattgtcggc gaacattttg gcgatgcacg gcaatggtta
    32221 caggccgatg tggaagatgc cgccatgaac tatcgtggct tcacattccc gttgtgggga
    32281 tttcttgcca ataccgatat ctcttacgat ccgcagcaaa ttgatgccca aacctgtatg
    32341 gcctggatgg ataattaccg cgcagggctt tctcatcaac aacaattacg tatgtttaat
    32401 cagctcgaca gccacgatac tgcgcgattt aaaacgctgc tcggtcggga tattgcgcgc
    32461 ctgccgctgg cggtggtctg gctgttcacc tggcctggtg taccgtgcat ttattacggt
    32521 gatgaagtag gactggatgg caaaaacgat ccgttttgcc gtaaaccgtt cccttggcag
    32581 gtggaaaagc aggatacggc gttattcgcg ctgtaccagc gaatgattgc gctgcgtaag
    32641 aaaagtcagg cgctacgtca tggcggctgt caggtgctgt atgcggaaga taacgtggtg
    32701 gtatttgtcc gcgtgctgaa tcagcagcgt gtactggtgg caatcaaccg tggcgaagct
    32761 tgtgaagtcg tgctacccgc gtcaccgttg ctcaatgccg tgcaatggca atgcaaagaa
    32821 gggcatgggc aactgactga cgggattctg gctttgcctg ccatttcggc tacggtatgg
    32881 atgaactaac gttttataac gccttgtgtg acgcctgcgc catcatccgc ggataaaact
    32941 gccagaagcg ggtttcgagg gcgtcataat gagcgtctaa atcgtaccag gagtcacgca
    33001 gggcatccag acgtgggcgg cggcttgcca tgccgtttaa cacgttctgg atgaaatcca
    33061 tatcgcgata gcgcaccagc cactgctctg accacaagta attgttcaga ttgataaaac
    33121 gtggcggtga gtccggcaaa atcgtcatca cttgctcgcg ggcataacag acaaattcct
    33181 gtagcggaaa gtccggcgac agctgcgacc agtggcggga aagaaagtga tcccacatga
    33241 catccagcgt aataggcgca acgcggcgag tttcactacg aaaccattcc cgtgcttcgc
    33301 ggacttccgg cagattgtcg gttaatacgt cgatacgtcg atgcatatga atgccagcca
    33361 cgacgtcggg cggaaaactt tcttcaggat ttccgcgtac gaaatcagcc agtaaattgc
    33421 cggaaagcga gctttccgcg agatgggcta aatgcaggtg agctaaaaaa ttcatcgatt
    33481 atattctatc caaaaggggg taaaggttgc agggagagcg ccccggcact agactacccg
    33541 cctcttattt tagtctgagt cagtgtcatg cgcgttaccg atttctcctt tgaattgccc
    33601 gaatccctga ttgcccacta tcccatgcct gaacgcagta gctgtcgttt actgtcgctg
    33661 gacgggccga cgggcgcgct gacgcacggt actttcaccg atttacttga taagctcaac
    33721 cccggcgatc ttctggtttt taataatacc cgcgtgatcc cggcgcgcct gtttgggcgt
    33781 aaagccagcg gcggcaagat tgaagtgctg gttgaacgga tgctcgacga caaacgcatt
    33841 cttgcgcata ttcgcgcctc gaaagcgcca aaacctggcg cagaactgct gctgggcgat
    33901 gacgaaagta ttaacgcaac aatgaccgcg cgccacggcg cactgtttga agtcgaattc
    33961 aatgatgatc gttcggtact ggatatcctc aacagtatcg gccatatgcc gctgccgccg
    34021 tatatcgacc gtccggacga agacgctgac cgcgaacttt atcaaaccgt ttatagcgaa
    34081 aaaccgggcg cggttgcagc cccgaccgct ggtctgcatt ttgacgagcc tttgctggaa
    34141 aaattgcgcg ccaaaggcgt ggagatggcg tttgtgacgt tgcacgttgg tgcgggcacc
    34201 ttccagccgg tgcgcgtcga caccattgaa gatcacatca tgcactcgga atacgctgaa
    34261 gtaccgcagg atgtggtaga cgcggtactg gcggcgaaag cgcgcggtaa ccgggtgatt
    34321 gcggttggca ccacttcagt acgttcgctg gaaagcgcgg ctcaggcagc gaaaaacgat
    34381 ctcattgaac cgttcttcga cgatacccaa atctttatct atccgggctt ccagtacaaa
    34441 gtggtcgatg cgctggtgac gaacttccac ttgccagagt cgacgctgat tatgctggtt
    34501 tcggcctttg ccggttatca acacaccatg aacgcctata aagcagcggt agaagagaaa
    34561 tatcgctttt ttagttacgg tgatgcgatg tttatcacgt acaatccgca ggcaattaat
    34621 gagcgcgtcg gggagtaatt ccgcggcgct ggtttaaaac gttggactgt ttttctgacg
    34681 tagtggagaa aaaatgaaat ttgaactgga caccaccgac ggtcgcgcac gccgtggccg
    34741 cctggtcttt gatcgtggcg tagtggaaac gccttgtttt atgcctgttg gcacctacgg
    34801 caccgtaaaa gggatgacgc cggaagaagt tgaagccact ggcgcgcaaa ttatcctcgg
    34861 caacaccttc cacctgtggc tgcgcccggg ccaggaaatc atgaaactgc acggcgatct
    34921 gcacgatttt atgcagtgga aggggccgat cctcaccgac tccggcggct tccaggtctt
    34981 cagccttggc gatattcgta aaatcaccga acagggcgtg cacttccgta acccgatcaa
    35041 cggcgatccg attttcctcg atcctgaaaa atcaatggag attcagtacg atcttggttc
    35101 ggatatcgtc atgatctttg atgagtgtac gccgtatcct gctgactggg attacgcaaa
    35161 acgctccatg gagatgtctc tgcgttgggc gaagcgtagc cgtgagcgtt ttgacagtct
    35221 cggaaacaaa aatgcgctgt ttggtatcat tcagggcagc gtttacgaag atttacgtga
    35281 tatttctgtt aaaggtctgg tagatatcgg ttttgatggc tacgctgtcg gcggtctggc
    35341 tgtgggtgag ccgaaagcag atatgcaccg cattctggag catgtatgcc cgcaaattcc
    35401 ggcagacaaa ccgcgttacc tgatgggcgt tggtaaacca gaagacctgg ttgaaggcgt
    35461 acgtcgtggt atcgatatgt ttgactgcgt aatgccaacc cgcaacgccc gaaatggtca
    35521 tttgttcgtg accgatggcg tggtgaaaat ccgcaatgcg aagtataaga gcgatactgg
    35581 cccactcgat cctgagtgtg attgctacac ctgtcgcaat tattcacgcg cttacttgca
    35641 tcatcttgac cgttgcaacg aaatattagg cgcgcgactc aacaccattc ataaccttcg
    35701 ttactaccag cgtttgatgg cgggtttacg caaggctatt gaagagggta aattagagag
    35761 cttcgtaact gatttttacc agcgtcaggg gcgagaagta ccacctttga acgttgatta
    35821 atattaataa tgagggaaat ttaatgagct tttttatttc tgatgcggta gcggcaacgg
    35881 gtgcaccggc gcaaggtagc ccgatgtctt tgattttgat gctggtggta ttcggtctga
    35941 ttttctattt catgatcctg cgtccacagc agaagcgcac caaagaacac aaaaagctga
    36001 tggactccat tgccaaaggt gatgaagttc tgacgaacgg tggcctggtt ggtcgcgtaa
    36061 ccaaagtagc ggaaaacggc tacattgcta tcgcgctgaa tgacaccact gaagtagtta
    36121 ttaaacgtga cttcgtagct gccgtcctgc cgaaaggcac catgaaggcg ctgtaattaa
    36181 aatttttccc taagggaatt gccgtgttaa accgttatcc tttgtggaag tacgtcatgc
    36241 tgatcgtggt gattgtcatc ggtctgctgt atgcgcttcc caacctgttt ggtgaggatc
    36301 cggctgttca gatcactggt gcgcgcggag tcgccgccag tgagcaaacg ctgatccagg
    36361 tccagaaaac gttacaagaa gaaaaaataa ctgctaagtc tgtggcactg gaagagggcg
    36421 ctattcttgc gcgcttcgac tccactgaca cccagctgcg cgctcgtgaa gcattaatgg
    36481 gcgttatggg tgacaaatac gtcgtggcgc ttaaccttgc cccggcaacg ccgcgctggc
    36541 tggcagctat tcacgctgag ccgatgaagc tcggccttga cctgcgtggc ggcgttcact
    36601 tcctgatgga agtggatatg gacaccgcgc ttggcaaact ccaggaacaa aatatcgata
    36661 gcctgcgcag cgatctgcgt gaaaagggca tcccgtatac caccgttcgt aaagaaaaca
    36721 actacggcct gagcattact ttccgcgatg ctaaagctcg tgatgaagcc attgcgtatc
    36781 tgagcaagcg ccatccggac ctggtgatta gtagccaggg cagcaaccag ctgcgtgcgg
    36841 taatgagcga tgctcgtctg agtgaagcgc gtgaatatgc ggtgcagcag aacatcaata
    36901 tcctgcgtaa ccgtgtaaac caacttggcg tggcggagcc ggtggttcag cgtcagggtg
    36961 ctgaccgtat cgttgttgaa ctgccaggta ttcaggacac tgcgcgtgcg aaagagattc
    37021 tgggtgcgac ggcaacgctg gaattccgtc tggtaaacac caacgttgac caggccgctg
    37081 cggcatccgg tcgcgtaccg ggcgactctg aagtgaaaca gacccgcgaa ggtcagccag
    37141 ttgtgctgta caaacgcgta attctgaccg gtgaccatat caccgactcc acttccagcc
    37201 aggacgaata caaccagccg caggttaaca tctcgctcga tagcgctggt ggtaacatca
    37261 tgtctaactt cactaaggac aacatcggca aaccgatggc aaccctgttt gtggagtaca
    37321 aagacagcgg taagaaagat gcaaatggtc gtgcggttct ggtgaaacag gaagaggtga
    37381 ttaacatcgc caacatccag tctcgtctgg gtaacagctt ccgtatcacc ggcatcaaca
    37441 acccgaacga agcccgtcag ctgtcactgc tgctgcgtgc cggtgcgttg atcgcgccga
    37501 ttcagattgt tgaagaacgt accattggcc caaccctggg tatgcagaac attgaacagg
    37561 ggctggaggc ttgccttgcc ggtctgctgg tttctattct gttcatgatc atcttctata
    37621 agaagtttgg tctgattgcg accagtgctc tgattgccaa cttgatctta atcgtcggca
    37681 ttatgtcgct gttgccaggc gcaacgctga gtatgccagg tatcgcgggt atcgtcttaa
    37741 cccttgcggt ggcggtcgat gcgaacgtac tgatcaacga acgtattaaa gaagagttga
    37801 gcaacggacg tactgttcaa caggcaattg atgaaggtta tcgtggcgca ttcagttcta
    37861 tcttcgatgc gaacatcacc acgctgatta aagtcatcat cctgtacgca gtgggtaccg
    37921 gggcaattaa agggttcgcg attactaccg gtatcggtgt ggcgacgtcg atgtttaccg
    37981 cgattgtcgg tacgcgtgcc atcgtaaacc tgctatatgg cggcaagcgc gtcaagaagc
    38041 tgtcaatctg aggagtgcga tgtggcacag gaatatactg ttgaacaact aaaccacggc
    38101 cgtaaagtct atgactttat gcgctgggac tactgggctt ttggcatctc tggtctgctg
    38161 ttaatcgccg ctatcgttat tatgggcgtg cgcggcttta actgggggct ggatttcacc
    38221 ggtggtacgg ttatcgaaat tacgctcgaa aaaccggctg aaattgacgt aatgcgtgat
    38281 gcattgcaaa aagccggttt tgaagagcca atgctgcaaa actttggtag cagccacgac
    38341 atcatggtcc gtatgccgcc tgctgaaggc gaaaccggcg gtcaggtgct gggcagccag
    38401 gttctgaagg tgattaacga atccaccaat cagaatgcag cagtgaagcg tattgagttc
    38461 gtcggtccga gcgtgggggc agaccttgcg caaaccggtg cgatggcgtt gatggcggcg
    38521 ctgctgtcta tccttgtgta cgtaggtttt cgctttgagt ggcgactggc ggcaggggtt
    38581 gttattgcgc tggcgcacga cgttatcatt acgctgggta ttttgtcgtt attccatatc
    38641 gagattgacc tgaccattgt ggcatcgttg atgtcggtta tcggttactc gcttaacgac
    38701 agtatcgtgg tatcggaccg tattcgtgaa aacttccgca agatccgtcg cggtacgcct
    38761 tacgaaatct ttaacgtgtc cttgacccag acgctgcacc gtaccttgat cacatccggt
    38821 actaccttga tggttatcct gatgctgtac ctcttcggtg gtccggtact ggaaggcttc
    38881 tcgctgacca tgcttatcgg tgtttccatc ggtactgcat cttccatcta tgtggcatct
    38941 gcgttggctc tgaaactggg tatgaagcgc gaacacatgt tgcagcagaa agtggaaaaa
    39001 gaaggggcgg atcagccgtc aattctgccg taatcaagtt cccgttgatg ttgaaaatcc
    39061 cggtcagaag atcgggattt tttttgatgt atggagattg cgaaaatccc cgcatcttgg
    39121 gaaactgcgc gtaaccctac atttcatcca ggtaactctt catggctatc atcccaaaaa
    39181 actatgcgcg gttagaaagc ggctatcgcg aaaaagcatt aaaaatatat ccgtgggtct
    39241 gcggtcgctg ttcccgcgag tttgtttatt ccaacctgcg tgaacttact gttcaccaca
    39301 ttgatcacga ccataccaat aacccggaag atggcagtaa ctgggaattg ctttgtctct
    39361 attgccacga tcatgagcat tcgaaatata ccgaagcgga tcagtatggt acgaccgtta
    39421 tcgcagggga agatgcgcag aaagatgtcg gtgaagcgaa gtacaaccca ttcgctgacc
    39481 tgaaagcgat gatgaacaag aagaagtgat taaaacgtaa aattgcctga tgcgctacgc
    39541 ttatcaggac tacgttattt cagcaatata ttgaatttac gtgcttttgt gggtcggaca
    39601 aagcgtttac gccgcatccg gcatgaacaa agcgcacgtt gttaacaatc tgaaatgccg
    39661 ggaataaatc ccggcatttt cataatcaga agttgtaacc tacaaccagg taaccacccc
    39721 agccggtaga gcgaacgttg aagttgccgt tgccgaagtt cagttctgca tcgtcgttcc
    39781 actgaccacc gtcgtgccag taacgagcta cgacagagta gtgccagtga tcgtagttca
    39841 gagccagaat atggctggaa gcgatagagt tattagtacg ggtcttaata tcgttgattg
    39901 cgttaccgct gtcatcccct aaatcggaac cccagtcgaa gttggtgaag ccgatgtagc
    39961 tcagctgacc gccccacaga tcggtaatcg gcacaaagta tttaattttg aaacggtaac
    40021 cgtcccactc gttttcgttc gctgcgccat agttctgcca ctggtatttc gcatagacgt
    40081 tcatggacag gctcatcggc agaccagtat cgatgtcggt acccagaccc atgtaccagg
    40141 tgctctggcg accatcttta ttacgaccca tgtcgtaaat gtagttgttc gcgaagtacc
    40201 actctttgaa cggaccgaag ctaaggtcag tattggtcag cttgtcgatg gagaaacgtg
    40261 gttcgatttc cataaacagc ggagaaccgt ggttccagat acctttagca tcggagttac
    40321 cgccgaagaa taccggcgca tccgcataac catagaagtc gaaccagtct tttttagcga
    40381 atgcttcgta ctcaaggtag gtatcgttgc ggatctgcgg tccgaaacgg gtgtgatagc
    40441 ttccgacaac gttaacgctc tggtgccacc agtcggaaag atactgcggt ttgtcgtttt
    40501 cagctgcgtt gacagtaaaa gacgaagaga gcgccagtac cgcaccggct gccagtaatg
    40561 tttttttcat atgtatgcca ctgtttgaaa atcccttgcg ggagtgaaaa aggtgcaaat
    40621 tgcgtttcta aatatttcgt atacagagga gcctattata aaaatcattg ctcacaaaaa
    40681 tatgtttcgt ttcacagttc tatcatttac gtaatcgatt gcgttcacgt ttacacacat
    40741 tcgggcaggg attgtactga ctttcaccct tgttgcaaac ttttactatc aatcagggtg
    40801 atgtaagcgg agtaacaaaa tgacggggaa agatgttgcg agacgcacaa aatcgccccg
    40861 aaatacgttt actgcataac aggttgaata tcgtggatac gtacgaagtc taactgttct
    40921 acagaaatac cctttaactg taacggaata tcaacatcgc tgggggcgag gacgctggca
    40981 ggggcattaa tcaattgatt ctgcacattg atttcctgaa agttgtctat tgttccctga
    41041 atttgcccat attcaacagt tccgctgaat gcgggcaagg gatcattcga ctcgccctga
    41101 atacgtagcg tcagggtggt gccatctgca tcaggcgtaa tattcaccag cgacatacgt
    41161 aaagtaccga tctggctttc cagtcttgcc ggtgttttcg ctcctggcag aaggtaaacc
    41221 ccactgctgg attttgcatt cagcctgttt tgctgagtga ttttgaccgt ttctttattg
    41281 agttgcgtca tctcttggtt cagcgtgctg acgctgtgtt tcatctgtcg cacttcgctc
    41341 tgctgcacac aggcgctaag gctgaacagg cttcccagca ggagcagtcg aaaaacgtta
    41401 gtattcatag gtcgtccctt tctcgtcgca attagcctaa tggtagaggg taaaagcagc
    41461 gaaagcatcg atcctttatc tcaaaagcgt tgcgcctttg ttgtatcgtc agttcagggt
    41521 aaaatagatt tctgttaacc acctggtcag gacgccgtat gcattgccca ttctgtttcg
    41581 ccgtggacac taaggtaatt gactctcgtc tcgtgggcga gggttcatcc gtacgccgcc
    41641 gtcggcagtg tctggtgtgt aatgaacgtt tcaccacctt tgaagtggcg gagctggtta
    41701 tgccgcgtgt tgtaaaaagc aacgacgtgc gtgaaccgtt taatgaagag aaattgcgta
    41761 gcggaatgct gcgggcgctg gaaaaacgtc cggtgagttc cgatgacgtc gaaatggcaa
    41821 tcaatcatat taaatcgcag ctgcgcgcca ccggtgagcg cgaagtgccg agcaagatga
    41881 ttggcaatct ggtgatggag caattgaaaa agctcgataa agtcgcctat atccgttttg
    41941 cctctgtcta ccgcagtttc gaagatatca aagaatttgg cgaagagatc gcgcgcctgg
    42001 aggactaagc cgtgcaggac gagtattaca tggcgcgggc gctaaagctg gcgcaacgag
    42061 gacgttttac cacgcatccc aacccgaatg tcgggtgcgt cattgtcaaa gatggcgaaa
    42121 ttgtcggtga aggttaccac caacgtgcgg gtgaaccaca tgccgaagta cacgcgttgc
    42181 gtatggcggg tgaaaaagcc aaaggtgcga ccgcctatgt cacactcgaa ccctgtagcc
    42241 atcatggtcg tacgccaccg tgctgtgacg cactcatcgc cgctggcgta gcgcgcgtgg
    42301 ttgcctcgat gcaagatcct aacccgcagg tcgctgggcg tggactttac cgtctgcaac
    42361 aggctggcat tgacgtcagc cacgggctga tgatgagtga agccgagcaa ttgaataaag
    42421 gctttctcaa gcggatgcgc accggctttc cttatattca gttaaaactt ggcgcatcgc
    42481 ttgatggccg cacggcgatg gcgagcggcg aaagccagtg gatcacttcg cctcaggcgc
    42541 ggcgcgacgt acaacgacag cgcgcacaaa gtcatgccat tttaaccagc agcgccacgg
    42601 tgctggcgga tgatccagcc ttaacagtgc gttggtctga actggatgaa caaactcagg
    42661 cgctctaccc gcaacaaaat ctccgccagc cagtacgtat tgtgattgat agccaaaatc
    42721 gcgtgacgcc ggaacatcgc attgtgcagc agcccggcga aacctggttc gcgcgtacgc
    42781 aggaagattc tagtgagtgg ccggaaacgg tgcgtacctt gctgattcca gagcataaag
    42841 gtcatctgga tctggttgta ctgatgatgc aactgggtaa acagcaaatt aacagcatct
    42901 gggtggaagc ggggccaacg ctcgctggcg cattgctgca ggcgggttta gtcgatgagc
    42961 tgattgtcta tatcgcacct aaactattag gcagcgacgc ccgcggatta tgcacgctgc
    43021 cagggcttga gaaattagcc gacgcccccc aatttaaatt caaagagata cgtcatgtag
    43081 gcccggatgt ttgcctgcat ttagtgggtg catgatctcc cggctcgaaa gggaagcagc
    43141 gcacgaaata ttatgctaaa atccgccccc ctgcggggcc atactcgaac ccgaaggaag
    43201 aaaatgaaca ttattgaagc taacgttgct accccggacg ctcgcgtcgc catcaccatc
    43261 gcgcgtttca acaactttat caatgacagc ctgctggaag gtgcaattga cgcactgaaa
    43321 cgtatcggtc aggtaaaaga tgaaaacatt accgttgttt gggtgcctgg tgcctatgag
    43381 ctgccgctgg cggcgggtgc actggctaaa accggtaaat acgacgcggt gattgcgctg
    43441 ggtacggtta ttcgtggtgg cactgcccat tttgaatatg tcgctggtgg tgcaagcaac
    43501 ggcctggcgc atgttgccca ggacagcgaa attccggttg cttttggggt tctgaccact
    43561 gaaagcattg aacaagcgat cgaacgtgct ggcaccaaag ctggcaacaa aggtgcagaa
    43621 gctgcactga ccgcgcttga aatgattaat gtattgaaag ccatcaaggc ctgaaattag
    43681 taaggggaaa tccgtgaaac ctgctgctcg tcgccgcgct cgtgagtgtg ccgtccaggc
    43741 gctctactcc tggcagttgt cccagaacga catcgctgat gttgaatacc agttcctggc
    43801 tgaacaggat gtaaaagatg ttgacgtcct gtacttccgt gagctgctgg ccggggtggc
    43861 gactaacacc gcatacctcg acggactgat gaagccatac ctgtcccgcc tgctggaaga
    43921 actgggacag gtagaaaaag cagtactgcg cattgcgctg tacgagctgt ctaaacgtag
    43981 cgatgtgcca tacaaagtgg ccattaacga agcgatcgaa ctggcgaaat cgttcggcgc
    44041 agaagacagc cataagttcg tcaacggcgt actcgataaa gcagcacctg tgattcgccc
    44101 taacaaaaag taatatccag gccggtagat tcacggaaga ccgttccatg atcgccggcc
    44161 ttttcttttt tacctgctga ggcataacgt atggcatgtg gcgagttctc cctgattgcc
    44221 cgttattttg accgtgtaag aagttctcgt cttgatgtcg aactgggcat cggcgacgat
    44281 tgcgcacttc tcaatatccc cgagaaacag accctggcga tcagcactga tacgctggtg
    44341 gcgggcaacc acttcctcca tgatatcgat cctgctgatc tggcgtataa agcactggcg
    44401 gtgaacctaa gcgatctggc agcgatgggg gccgatccgg cctggctgac gctggcatta
    44461 accttaccgg acgtagacga agcgtggctt gagtccttca gcgacagttt gtttgatctt
    44521 ctcaattatt acgatatgca actcattggc ggcgatacca cgcgtgggcc attatcaatg
    44581 acgttgggta tccacggctt tgttccgatg ggacgagcct taacgcgctc tggagcgaaa
    44641 ccgggtgact ggatctatgt gaccggtaca ccgggcgata gcgccgccgg gctggcgatt
    44701 ttgcaaaacc gtttgcaggt tgccgatgct aaagatgcgg actacttgat caaacgtcat
    44761 ctccgtccat cgccgcgtat tttacaggga caggcactgc gcgatctggc aaattcagct
    44821 atcgatctct ctgacggtct gatttccgat ctcgggcata tcgtgaaagc cagcgactgc
    44881 ggcgcacgta ttgacctggc attgctgccg ttttctgatg cgctttctcg ccatgttgaa
    44941 ccggaacagg cgctgcgctg ggcgctctct ggcggtgaag attacgagtt gtgtttcact
    45001 gtgccggaac tgaaccgtgg cgcgctggat gtggctctcg gacacctggg cgtaccgttt
    45061 acctgtatcg ggcaaatgac cgccgatatc gaagggcttt gttttattcg tgacggcgaa
    45121 cctgtcacgt ttgactggaa aggatatgac cattttgcca cgccataaag atgtcgcgaa
    45181 aagtcgcctg aagatgagta atccgtggca tctgcttgct gtcggattcg gaagtggatt
    45241 aagcccaatc gttcctggca cgatgggatc gctggcagcg attccgttct ggtatctgat
    45301 gaccttttta ccctggcagc tctactcgct ggtggtgatg ttggggatct gtatcggcgt
    45361 ctatctttgt catcaaacgg cgaaagatat gggcgtgcac gatcacggca gcattgtctg
    45421 ggacgaattt attggtatgt ggatcacgct catggcgctg ccgaccaatg actggcagtg
    45481 ggttgccgcc gggtttgtga ttttccgtat tctggatatg tggaagccgt ggccgatccg
    45541 ctggtttgat cgcaatgtac atggcggcat ggggatcatg atcgacgata ttgtcgccgg
    45601 ggtgatttcc gcaggcatcc tgtactttat cggtcatcac tggccgctgg gtattctgtc
    45661 gtagttgtgc accgatgcct gatgtgacgc ttgtcacgtc tcatcaggcc tggactctta
    45721 tttaaatcct acgaccgcat gcggtttata cggcgtttcc agttcggcaa tctgttctgg
    45781 cttcaaagtg atatccaccg cgttcagcag ctcatcaagc tgttcttccc gcgatgtacc
    45841 gataatcggt gcggcaatgc ccggtttact caacaaccag gccagcgcaa cttgtgctcg
    45901 tgttgcaccg agttcttcac tgacgcccgt taaccgctct gcgatctgcg cgtcattttc
    45961 atcgctttct ttatagagat ttctccccac ctcatcagac accagtcgtg cggtagtttc
    46021 tccccacgga cgcgtcagtc gcccccgcgc cagcgggctc cacggaatca ccgccacgcc
    46081 ctcctgataa cacagaggca gcatctcgcg ctcttcttcg cgataaatca gattgtagtg
    46141 atcctgcata ctgataaact gcgcccagcc gtgctgtttt tgtagcttca gggcctgagc
    46201 aaactgcgaa gcgtgcatgg atgacgcgcc gatataacgc gctttcccgg cttttaccac
    46261 gtcgttcagg gcttccagcg tctcttcgat cggcgtgttg taatcccagc gatgaatttg
    46321 caggatatcg acataatcca tgccgagacg gcgcaggctg tcgtcgatag agcgcaaaat
    46381 ttgtgcacgg gataatcctt ccggtaaatc accaacgcga tggaacactt tcgtcgcaac
    46441 gaccacgtct tcacgacggg cgaaatcccg cagtgcgcga ccaacgatct cttcgctgct
    46501 gccatcggaa aagctattgg cggtatcaaa gaaatttatg ccgccttcca gcgcgcgttt
    46561 aatgatggga cggctactct cttccggcag tgtccatgcg tgattaccac gatctggctc
    46621 gccaaaggtc atacagccga ggcaaagtcg ggaaacgcga aggtcggttt ttcctaaggg
    46681 gttgtattgc atgctgccac tcctgccata ctcgtcatac ttcaagttgc atgtgctgcg
    46741 gctgcattcg ttcaccccag tcacttactt atgtaagctc ctggggcttc actcgtttgc
    46801 cgccttcctg caactcgaat tatttagagt ctatgaataa tttgttaagc atagcaggag
    46861 tggagtaggg attatgccag ccaggccttg attttggctt ccataccggc ggcatcgagg
    46921 ccgagttcgg cgcgcatttc ttcctgagtt ccttgcggaa taaagaagtc cggcagacca
    46981 atgttcagca cgggtactgg tttacgatgg gccatcagca cttcgttcac tccgctacct
    47041 gcgccgccca taatggcgtt ttcttctacg gtgaccagcg cttcatggct ggcggccatt
    47101 tccagaatta acgcttcatc aagcggtttc acaaaacgca tatcgaccag cgtggcgttc
    47161 agcgattcgg cgactttcgc cgcttctggc atcagcgtac caaagttaag gatcgccagt
    47221 ttctcaccac gacgcttcac aatgcctttg ccaattggca gtttttccag cggcgtcagt
    47281 tccacgccaa ccgcgttacc gcgcgggtag cgcaccgcgg acgggccgtc gttatagtga
    47341 tagccggtat agagcatctg gcgacattcg ttttcatcgc tcggggtcat aatgaccatt
    47401 tccggtatac agcgcaggta agagaggtca aaagcgccct gatgggtttg accgtcagca
    47461 ccaacaatgc ccgcgcggtc gatggcgaac aggaccggga gcttttgaat cgccacgtca
    47521 tgcagcacct gatcataggc gcgttgcagg aaagtggagt aaatcgcgac aatgggtttg
    47581 tacccaccaa tcgccagacc ggcggcaaag gtcaccgcgt gttgctcggc gattgccacg
    47641 tcgaagtaac gatccgggaa tttacgtgaa aactcgacca tgccggaacc ttcacgcatc
    47701 gccggagtaa tcgccatcag cttgttgtct ttcgctgccg tttcgcacaa ccagtcgcca
    47761 aagatttttg aatagctcgg caaaccgccg ctacttttcg gcaaacaacc gctggaggga
    47821 tcaaatttag gcacggcgtg gaaagtgatg gggtcttttt ctgccggttc ataaccacga
    47881 ccttttttgg tcatgatatg caggaactgc gggcctttca ggtcgcgcat gttcttcagc
    47941 gtggtgataa gccccagcac atcgtgaccg tcaaccgggc cgatgtagtt aaagcccagc
    48001 tcttcaaaca acgtgccagg cactaccata cctttaatat gttcttcggt acgtttgagc
    48061 agctctttaa ttggcggaac gccagagaaa acttttttcc cgccttcgcg cagcgaagag
    48121 taaagcttac cggaaagcag ctgtgccaga tggttattga gcgcgccgac attttcggaa
    48181 atcgacattt cattgtcgtt gaggaccacc agcatatcag gacggatatc gcccgcgtga
    48241 ttcatcgctt caaacgccat gccagcggta atcgcgccat cgccaatgac acagacggtg
    48301 cggcgatttt tgccttcttt ctcggcagca accgcaatac caattccggc actgatggag
    48361 gttgatgaat gcccgacgct taatacgtca tattcgcttt cgccgcgcca cgggaacggg
    48421 tgcaggccgc ctttctgacg gatggtgccg attttgtcgc ggcgtccggt caaaatttta
    48481 tgcggataag cctgatgccc cacatcccaa atcaattggt caaacggggt gttgtagaca
    48541 tagtgcagcg ccacggtcag ttcgaccgtg cccagcccgg aggcgaagtg cccgctggaa
    48601 cggctcacgc tgtcgagtaa atagcggcgc agttcgtcgc agagtttcgg taaactctct
    48661 ttcggcaaca gtcgtaactc ctgggtggag tcgaccagtg ccagggtcgg gtatttggca
    48721 atatcaaaac tcatcagggg cctattaata cttattgttt atttattacg ctggatgatg
    48781 tagtccgcta gcgcttccag tgccgaggta tcgagtgatt gttcagccag ttgtttcagc
    48841 gactggcggg catcgtcgat cagatcccgg gctttcttcc gggcttgctc aagacccaga
    48901 agtgcagggt aggtactttt accaagttgc tggtcggcac cctggcgttt tcccaacgtt
    48961 gcagtatctc ctaccacatc caggatgtca tcctgaacct ggaaggcaag gccgatgctc
    49021 tctgcgtact tgtcgagtac tggcagagca cgacgccctt tatctccggc gcttaatgca
    49081 ccaaggcgaa cggcggcgcg aatcaatgcg ccggttttat gacgatgaat acgctcaagc
    49141 gcgtccagag gtacgtgttt gccttccgcg tctaaatcta atgcctgacc accgcacatt
    49201 ccggcaatac cgctggcgct cgccagttca gaaatcatcg aaattctgtc gcgatccgac
    49261 acttccggca tattggcatc gcttagaatc gagaacgcca gcgtttgtaa agcgtcgcca
    49321 gcgagaatcg cgtttgcttc gccaaacttc acatggcagg tcggcaaacc gcgacgcaga
    49381 tcgtcatcat ccatcgccgg taaatcatca tgaattaatg agtaagcgtg gatacactct
    49441 acggcggcag cgggtgcgtc cagcgtgttt gtgctaacgc caaacatatg accggtggca
    49501 taaaccagga agggtcgcag acgcttacca cctaataatg cgccatactg catggtttcg
    49561 accacgggag tgttctgaaa gggcagtggg gcgataaaac ggctcagcgc ctggttggcc
    49621 tgcttaacgc aggcttcgag ttgctgcgga aagtccatta ctcattgtcc ggtgtaaaag
    49681 gggttagaga ggcgtcttca ttgtcagaca gcagaatttg tacgcgctgt tcggcttgtt
    49741 gtaatttggc ctgcccctga cgtgccagct gcacgccgcg ttcgaactcg ttcagcgcct
    49801 cttccagcgg caggtcgcca ctttccagac gggttacaat ctgttccagc tcgctcagcg
    49861 ccttttcaaa gctggcggga gcctcatttt tcttcggcat aatgaatgtc tgactctcaa
    49921 tatttttcgc cccgtcatgg taacggactc agggcaaata gcaaataacg cgcaatggta
    49981 aggtgatgtg cgcagcaaag cgatgttagt ggtatacttc cgcgcctgga tgcagccgca
    50041 ggtgtgggct gctgtatttt tccctataca agtcgcttaa ggcttgccaa cgaaccattg
    50101 ccgccatgaa gtttatcatt aaattgttcc cggaaatcac catcaaaagc caatctgtgc
    50161 gcttgcgctt tataaaaatc cttaccggga acattcgtaa cgttttaaag cactatgatg
    50221 agacgctcgc tgtcgtccgc cactgggata acatcgaagt tcgcgcaaaa gatgaaaacc
    50281 agcgtctggc tattcgcgac gccctgaccc gtattccggg tatccaccat attctcgaag
    50341 tcgaagacgt gccgtttacc gacatgcacg atatttttga gaaagcgttg gttcagtatc
    50401 gcgatcagct ggaaggcaaa accttctgcg tacgcgtgaa gcgccgtggc aaacatgatt
    50461 ttagctcgat tgatgtggaa cgttacgtcg gcggcggttt aaatcagcat attgagtccg
    50521 cgcgcgtgaa gctgaccaat ccggaagtga ctgtccatct ggaagtggaa gacgatcgtc
    50581 tcctgctgat taaaggccgc tacgaaggta ttggcggttt cccgatcggc acccaggaag
    50641 atgtgctgtc gctcatttcc ggtggtttcg actccggtgt ttccagttat atgttgatgc
    50701 gtcgcggctg ccgcgtgcat tactgtttct ttaaccttgg cggcgcggcg catgaaattg
    50761 gcgtgcgtca ggtggcgcat tatctgtgga accgttttgg cagctcccac cgcgtgcgtt
    50821 ttgtcgctat taatttcgaa ccggtcgtcg gggaaattct cgagaaaatc gacgacggtc
    50881 agatgggcgt tatcctcaaa cgtatgatgg tgcgtgccgc gtctaaagtg gctgaacgtt
    50941 acggcgtaca ggcgctggtc accggcgaag cgctcggcca ggtgtccagt cagacgctga
    51001 ccaacctgcg cctgattgat aacgtctccg atacgctgat cctgcgtccg ctgatttctt
    51061 acgacaaaga gcacatcatc aacctggctc gccagattgg caccgaagac tttgctcgca
    51121 cgatgccgga atattgcggt gttatctcca aaagcccgac ggtgaaagcg gttaagtcga
    51181 agattgaagc ggaagaagag aaattcgact tcagcattct cgataaagtg gttgaagaag
    51241 cgaataacgt tgatatccgc gaaatcgccc agcagaccga gcaggaagtg gtggaagtgg
    51301 aaaccgtcaa tggcttcggc ccgaacgacg tgatcctcga tatccgttct atcgatgaac
    51361 aggaagataa gccactgaaa gtcgaaggga ttgatgtagt ttctctgccg ttctataaac
    51421 tgagcaccaa gtttggcgat ctcgaccaga acagaacctg gctactgtgg tgtgagcgcg
    51481 gggtgatgag ccgtctgcag gcgctctatc tgcgcgagca gggctttaac aatgtgaagg
    51541 tatatcgccc gtaatttgtg gttgtaacgt cgcatctggt cagatgcgac gcttaccgca
    51601 tccgacacta ctcgtaataa ttataaatcc ctgccgccat caccagttgt gatgccactt
    51661 catgggcttt ctcgcgccca accagcaggt cgataatttt cagaccaaag tcgatagctg
    51721 tccccggccc ctggctggtc agcaatttta cccgtgcatc ccagacgacg cgcttgtcct
    51781 gccattgttc agcgggaatt ttgtctttca gcgtcgggaa gccggtcata ttaccaatcg
    51841 ggaagatatc gtgcggcacc agcacggtgg ctggcgcggc gcaaatagcc gcgacgatac
    51901 gcccggaacg gtggaactgt ttaacggttt caaccagcag ggtactatcg cgaaaacact
    51961 ctgcgccttt aatgccacca ggcagtacga tcacgtcata ttcgccatca gccacctcga
    52021 ccagcggcgc atccgccagc agcttcacgc cgcgcgagca ggtaatggcc aggttgccat
    52081 cgctggcgac gctggcagtg gtgactttga taccgccgcg aaccagcaga tcgatagtgg
    52141 tgacggcttc agtctcttca ctaccagggg cgaggcaaac cagtgccgat gcgctcatat
    52201 tcactctcct ttctttttac catttcaaac aggcgggtgt tttccggtac ggcaatccca
    52261 tgcgcgcggg cgcggcgtaa gagaaaacca ttgatatagt cgatttcggt gtggcgcagc
    52321 gcgcggatat cctgcaacat cgacgagata ttttccgctg tggcatcaat cacctgcatc
    52381 acgtaatcac gcaaatcttc tgctgaagta tgatgccctt cgcgctcgat cactgccgcg
    52441 acttcttcgc atatctgcat aatttcttgc ggatgatgac gtaattcacc attcgggcaa
    52501 ttccagatgg cggtcagtgg attaatcaca cagttgacgg ccagcttgcg ccacagctcg
    52561 gcgcgaatat tgttatgcca agcaacgtca ggcaacacgg tttgcaaaat atccgccaga
    52621 taactgtaat cgccatcctg ttgccgtgcc ggaccgatat gcgtgattcc gtttgccaca
    52681 tgaataatga cattgccgtc gcggcgggcg gcatgggtgg tggtgcccat cagtaatggc
    52741 tgctgaatgt tttgcaattc ttcgatggtg cccatgccgt tgtgaattaa cagtattggc
    52801 gtagctacag gcagtgtgga tgcgaggctt ttgacggcat cggaaacctg ccatgctttc
    52861 agcgtcacca ggagcagatc gctggtggcg agaaaatcgg gatcgttggc ggtcagcgat
    52921 tcgttaaata tcgaaccatc tgtctcaacc agattcacac tacaataagg ttgcggtacg
    52981 cgcagccagc cctgaacttc gtgaccctgt ttacaaagtg ctgtaagcca taattgccct
    53041 aaggcaccgc atcccaatac ggtaattttc attgttcctc ctcacccgca accactccgg
    53101 gtgttcaata aggctatccc ttaattgtgc atgctgttgc gactatgcac aattaaggga
    53161 tacgtcctgg tgcaggactg ttggttattt aactttgcag gtattatgct tcgcatcaaa
    53221 aatgaaggga gaggaaaaga tgccatcttt cgatattgtc tctgaagttg atcttcagga
    53281 agcacgtaac gcggtcgata acgcgagtcg cgaagtggag tcccgttttg acttccgtaa
    53341 cgttgaagcc tcatttgagc tgaacgacgc cagcaaaacc atcaaagtgt tgagcgagtc
    53401 cgacttccag gtcaatcagt tgctggatat tctgcgtgcc aagctgctga agcgcggcat
    53461 tgaaggcagt tcactggatg taccggaaaa tatcgttcat agcggtaaaa cctggtttgt
    53521 ggaagcgaaa ctgaaacagg gcattgagag cgcgacccag aagaaaatcg tcaagatgat
    53581 taaagacagc aaactgaaag tgcaggcgca aattcagggc gatgagatcc gtgtgactgg
    53641 caaatctcgt gatgatttgc aggctgtcat ggcgatggta cgtggtggcg atctcggcca
    53701 gccgttccag ttcaaaaact tccgcgatta atcgcgacaa tgtgcgtttt gttcatgtca
    53761 gatgcggcat gaacgcctga ttcggcctac aacggtttgt aactcaagaa ctttgtaggc
    53821 ctggtaagcg tagcgcagca ggcaattcgc cgcgtttttt atgcctgacg aattgcctgt
    53881 tcaacctcaa agcgatttgt cactttgctg tcgattttca cataagctga atgctcttct
    53941 tctgcaatca acacttcttt gacgccttca gtttccagca aacgcacttt taacgcttcg
    54001 tttgcggcaa tgtccgccgg gatttcaatg cgcaaactgc tgacatacgg cggttctttc
    54061 atggtactgg cgactgccag ccacactgcg gccagcattg cgccagcgag aaataccccc
    54121 tgaccgtcaa acatgccgtc aatccagccg cccagcgaac cgccaatcgc cacgccaaga
    54181 aactggctgg tggagtagac acccatcgcc gtacctttgt aacctgctgg cgactcttta
    54241 ctgataagtg agggcagaag ggcttccatc aaactaaacg ccacaaagaa aagctgcacg
    54301 ccgaccacca gttgccagaa ctgcgtttgt gcgttccaca acacaatttc cgcaaccacg
    54361 atcaacccga cgcagaagac aaagacttgc ttcattttgc gcttaacttc agcgtagata
    54421 atgaaaggca cgaccgagcc aaaggcgatt aacatcgtcg ccagatagac cttccagtgt
    54481 tcagccgccg ggaaccccgc atcagctagc tgtccgggca gggcaacaaa cgtagacatc
    54541 agaagcatgt gcagacacat aatgccaaag ttgagtttta gcagccgcgg ttccgccagc
    54601 actttactga aactgccttt caccattccg gactcacgat taagtacgtg agtgctactg
    54661 ttgggcacaa cccaaatggt caacgcaatg ccggttgttg ccagaatagc gatcatccag
    54721 aacagcgcgt gcagcccaag tttgtgagtg atgatcgggc caagcaccat cgcaatggca
    54781 aaggtaatgc caaagctcac gccgataaac gccattgctt tggtacggtt ttgttcgcgc
    54841 gtgagatcgg aaagcagcgc cataacggca gcggcaatcg caccggagcc ttgtagcgcc
    54901 cggcccagaa taattcccca gatggagtca gaaagcgcag cgataacgct accggcggca
    54961 aacaccgcca gcccaccgac aattaatggt ttgcgaccaa tgcggtcgga aagcaggcca
    55021 aacggaatct gaaaaacggc ctgagtcaga ccataaatac caatggcaat accgattaat
    55081 gcttcgctgg caccttgcag agccatgcca tacgtggtca gaaccggcag aaccataaac
    55141 atgcccagca tgcgcaacga gaaaacggtt cctaaacccc aggtcgcgcg ccgctcacct
    55201 ggcgtcattt tataatcgtt cattaccacc tctgttttaa attcgcgact agtgtaaagc
    55261 ggcaaacgaa tggggtaaac atgcggattt tttagtaaat atttcgagag ggatatgttt
    55321 ctaatgctaa gaaaaaaggt gccgtagcac ctttttaact gagaggtttt gttaccacac
    55381 agcagccagc agcgtatgcg agtccggtac cataaaatca acggacatca tcaccgagag
    55441 ggcagtgatg gcgatgatag agaagccgaa cagcttgcgc gcccagattc tgtcatcagc
    55501 aactttataa ccgcgcagag ccatacctaa ccaccagacg ctaaccgccg cggcgaccac
    55561 cagatattta tatccagcgt aaccgccaag agagagcatc agcgtggcaa cggcaaaggc
    55621 gatgatatac agcgtgatgt gattcttcgc caccgaaatg ccttttacca ctggcaatac
    55681 cggaatgttc gccgcctgat aatccttaaa gcggaaaatg gcgatggcat aggagtgagg
    55741 catctgccac aggctgaaga tagccagcag gatcgctgcg ccgctatcga actcgccggt
    55801 tactgcacag tagccaatca ccggcggcgc agcgccggag agcgaaccaa tcaatgtgcc
    55861 gtagactgag tggcgtttca tgtacaggct ataaacgccg acataaacga caaagcccat
    55921 cacccccagc cagcaggcca gcggattcgc gccaaaccac agcagcataa agccagcaaa
    55981 gcctagcaag gtggcgtaca ccagcgagac agcaggagag atcaggcctt taaccagtac
    56041 ccgattcttc gtcctttcca tctttctgtc gatatccctg tcgatgtagt tgttaaacac
    56101 acaacccgac gccacaacca gtgacacccc aaccagcgtg tagataaaca ggggataatc
    56161 aatgctgccc tttgaggcca gcaggaatcc cccaatcacc gagatcaggt tgccaaagat
    56221 gatgcctggt ttcgttactt gcaggtattg cttaaacatc ataaccgccg ctcttagtgc
    56281 atcatcatgt tgtagttgag gttccacata atccagatgg agcctacaac caggatagcg
    56341 atgattagca cggtgaagac aaacgccgtc atgttccagc cttcatctga tttggtattc
    56401 atgtgcagga agcacaccag atgcaccaga acctgtacca ctgccattgc caggattgtt
    56461 cccagaatta cggccggaga ggcagcccct gtcatcacca tccagaacgg aatcaccgtc
    56521 agaatgatcg acaggataaa gcctgtcatg taggttttta cgctgccatg ggacgcgccg
    56581 ctgtgatcgg tagaatgact cattacatcg cccccatcag ataaacaaca gtgaacacac
    56641 agatccaaac cacatccagg aagtgccaga acaggctcag gcacatgatg cgggtacggt
    56701 tagtgctggt caggccgcga cgagcgattt gtaccatcag caccgccatc cagataagac
    56761 cggaagtgac gtgcagaccg tgcgtgccga ccagcgcaaa gaacgctgac aggaagccgc
    56821 tgcgatccgg acccatgccg ttaacaatca ggtgatggaa ttcgtagatt tccatcccga
    56881 taaatccggc gccaaacaac caggtcaacg ccagccagga gataacctgg cttttgttgt
    56941 ttttgtacat ggcgatagcc gccatgccgt aggtgatgga gctgaacaac agcaagaaag
    57001 tttcaaccag aacgaacggc agttcgaaaa tgtccttacc tgtcgggccg cctgcggtgc
    57061 cgttcaccag aacggcatag gtagcaaaca agatagagaa cagaatgcag tcgctcatca
    57121 ggtagatcca aaatccgaag attttggttc cgcctgcatc gtggtgcccg tgttcgtgcg
    57181 cgtgggcagt cgcgtgcgtc aaagtatcag ttgccatttt tcagccctgc cttagtaatc
    57241 tcatcgaaat gctggttttc cagtttttcg atttctgcca ccggcacgta gtaatccacg
    57301 tcctcgtcga agcttttcac gatccaggtg atgatcatgc ctgcgaagcc aacaatcgcc
    57361 agccaccaga tatgccagat catggcgaaa ccgaagatgg tggagaaagc tgcaatgacg
    57421 atacctgcac cgctgttttt cggcatatga atttcttcat agtggtcagg ctttttgtac
    57481 gcttcgcctt tctctttcat ttcccagaat gcatcacgct cgtgaacgtg cggcacaacg
    57541 gcaaagttat agaacggagg cggggaagag gttgcccact ccagcgtacg gccaccccac
    57601 gggtcgccag tcaggtcacg gttctggtcg cggtcacgaa tagaaacgta catctgaata
    57661 acgaggcaga gaatacccag cgcaatcagt actgcaccgc tggctgcaat catcagcatg
    57721 gtgtggaact gcgggtcaat ctgctggctc aaacggcggg tcatacccat gaagcccagc
    57781 gcatacagtg gcataaaggc aacgaagaag ccgatgatcc agaaccagaa cgcgcgttta
    57841 ccccaggttt cgttcagttt gaaaccgaac gctttaggcc accagtaggt catccctgcg
    57901 aagcagccga agaccacgcc gccgatgatc acgttatgga agtgcgcaat caggaacaga
    57961 ctgttatgca gaacgaagtc cgcgcccggt actgccagca gcacgccagt cataccgccc
    58021 accgagaagg tgacgataaa accgatagtc cacagcatcg cagaatggaa cacgatgcgg
    58081 ccctgataca tggtgaacag ccagttgaag atcttcaccc cggtcgggat ggcgataatc
    58141 atggtggtga taccaaagaa ggcgtttacg ttcgcgcccg cacccatcgt aaagaagtgg
    58201 tgcagccaga cgatgaacga cagcacggtg atacagacgg ttgcccatac cagcgaggta
    58261 taaccaaaca gacgtttacg cgagaaggtt gccgcaattt cggagaacac accgaaaaca
    58321 ggcaggatca ggatgtaaac ttccgggtgg ccccaggccc aaatcaggtt gatatacatc
    58381 atcatgttgc cacccatatc gttggtaaag aaatgggtgc ccagatagcg atccagggtc
    58441 aacaacgcga cggtaaccgt cagaattggg aaggaagcaa taatcagtac gttcgcgcac
    58501 agtgatgccc aggtaaatac tggcatcttg aacatggtca tgcccggtgc gcgcatcttc
    58561 agaatggtaa cgaagaagtt gataccggta agcgtcgtac ctataccgga tagctggaga
    58621 ctccatatcc agtaatcgac accgactccc ggactgtact ctattcccga tagcggtgga
    58681 taggccagcc agccggtctg cgcaaattcg cccacgccga gagaaacgtt aaccagaatc
    58741 acaccaacaa cggtaaacca gaagcttaag ttgttgagga acgggaacgc aacgtcacgc
    58801 gcgccaatct gcagcggaac caccaggttc atcagaccga taacgaaagg catcgccacg
    58861 aagaagatca taatcacgcc gtgcgcggta aagatctgat cgtagtggtg aggtggcagg
    58921 aagcccgctt cgcccgccga ggcaagagcc tgctggctac gcatcataat ggcgtcagca
    58981 aaaccacgca gcaacatcac aatcgccacg atgatataca tgataccgag gcgtttatgg
    59041 tcgacggagg tcagccactc tttccacagg taggtccact taccgaagta agtgatcagg
    59101 ccaacgagcg ccagacctcc caaaataatg ccagcgatcg taaccatgac gataggttca
    59161 tggaacggga ctgcatcaag tgataatttt ccgaacatct ttattcttcc tcaacccctt
    59221 taatgggcgg attccgcgtg gctcatgtcc atgccttcca taccttcgtg tgcgctgtgc
    59281 tcaccttctg gctgggtcat gtccatgctc ttaccgtgag ccataaactt gttaatcaca
    59341 tcggcaaaca agtctggttt cacgttggag aaatattcca cctggttgta ttcgctaggc
    59401 gcggccagtt tttcgaacgc tgccatgtca gacatggagt tcggcgactg tttcgctttt
    59461 gcgacccatt ggtcgaatgc ggcgcgatcc ggtgttgcaa tagctttgaa cttcatgcct
    59521 gagaagcccg ggccgctgta gctggcggag ataccgtcat aagtaccggg ttcgttggcg
    59581 atcagatgca ggcgagtctg cataccggcc atggcataaa tctggctacc cagacgcgga
    59641 atgaagaagg agttcatcac ggagttggag gtcactttga agtacaccgg agtgttcgcc
    59701 gggaaagcga tttcattcac ggtagcaatg ccctgttccg ggtagatgaa gaaccatttc
    59761 cagtccatgg aaaccacttc gatggtaatg ggcttctcgt cgtgtgccag cggcttgcta
    59821 ggctcaagag cgtgagtggt tttccaggtc agtaccgcaa ggaagatgat gattaagata
    59881 ggtaccgtcc agaccacagc ttccacttta ttggagtgtg accagttcgg gctgtactta
    59941 gcatctttat tgctcgcacg gtacttccag gcgaaaccaa cagccatcaa gattgcggga
    60001 ataacgacaa tcaacatcag gccaaatgcc gtcagtatca gtgaacgttg ctccagacca
    60061 atctgtcctt tgggatctaa cagcgcagaa ttacagccac tgagcaatac agtgcctgca
    60121 aataatgaca accatcccaa acttttattg tatttcctga gtctcattta acgacctcaa
    60181 ttccacggga tctggtggcg tttaaagtgt gtgggcattt tacgggaagg ttacattact
    60241 gtaaacatga ttaaattcct gttacttgga tttggctgtc aggtcacata tgttgcaaaa
    60301 catatcaggt tgattttaag aagctgttgc aaaagggcgt tataacaaaa ggggagtgca
    60361 gaaggagtct gggcgggcaa ttggtataac caatgcgaaa taaaacaatt atttaacaaa
    60421 taattatcat tggagtgaca actgggtgat gagaaaataa agactcatta gctgaatcgt
    60481 gtaagaaaaa tagaatttat aaatggcgtc aataatttcc aaacgctaag ccgcacaaaa
    60541 gaacaaatat tattaagcga tactgatgtt tatttagccg taataattac ggcgagtgat
    60601 tactacagct aaataatatt tacagattac gtcagatgcg tttttcgtag cgccagataa
    60661 tccagcaaac caccaagcac gacgccagaa agggcgacta aaaccccgac ttccagcaga
    60721 gcaggcagga aagagaagtg cgtcaaatcc agcgcgtcca tcgtcaacag cagtaaccac
    60781 acggccaaca ggctgacgcc cgccgccagt gtccacatgg caaaggcata acctgccgga
    60841 tattcggtac gggagataaa gttgtcattt actcgtgtat attcaagcgt ctggcggcaa
    60901 accagcagca aaataagccc cggtacggca gcggcgacgg agaatagata gaatgtcgac
    60961 cagccgtgtg cttcaacaaa ccaacccgcc acggggccga cataaactcg tcctacagcg
    61021 gaaagcgctg agagcagggc aaattgagta gcggaaaatg acttattaca cagcgtcatt
    61081 aacagcgcga caaaggctga tgtgcccatc ccgccacaga ggttttcgaa aaagacggct
    61141 gcgcccatgc tgtagagatg cttatcagta atcgacagca gccagtaacc agcgttagac
    61201 gcaccttgta aaatgccgaa aatcagcagc gcccggaaca gcgacaggcg ctgcatcaaa
    61261 atcccaccgt acaatgcgcc cacaatggtc gccagtaagc caagcgtttt gtttaccacg
    61321 cctacttcac ccgcatcaaa cccgacgccg cgaatcaaaa acgtggttgt caggctcatg
    61381 gcgaatgcgt cgcccagctt atacagcacg ataagaagca aaataagcca ggcattattg
    61441 cgaccaaaga aatctcgcag aggtgcaaca accgcttgtt ccagcgtttt gggcacagga
    61501 atggtgtcgg ttggttctgg tgcaagcaac gtcgcgataa tacaggggat caacagcgcc
    61561 gccatcaacc agtacatgcc ctgccagccc agccatttat ctgccagcca cagggctagg
    61621 ccgccggaaa ccagcatccc taaacggtaa cccagcacgc tgattgccgc gcccgcacca
    61681 cgctcttctg ccgggagcac atcggttttc cacgcatcga agacaatatc ctgagaggcc
    61741 gagcaaaaag cgatcaccac cgccagcgcc gccatccagc ggagttgggt gcctggttcg
    61801 agaaaaccca tcgccgcaat ggcgactaat aacaggattt gcgtggcgag cagccaaccg
    61861 cgccgccgcc caaaaaatgg gggcgtgtag cggtccataa gcggtgacca gaggaattta
    61921 aaaacgtaag cctggcctac cagagagaag aaaccaatgg ttttgagatc aatattctcg
    61981 accgtcatcc aggcctgtaa ggtaccggat gtcagggcga gcggtagccc ggaagcaaaa
    62041 cccaggatca gcaatatggc tgaacgcggc tgttgaaaaa tacgtaaata ttgactggac
    62101 atgggcgtct acaggcccgg cttgcaccgg gccagagggt agaaattaac gcgcgttctg
    62161 cttgatgaat tcgtggatgc tggtgtcctg agacatatca gcgatggtat cggtcagcac
    62221 actgttaacc gcatcggcga tatttttgtt ggaggcctgg aacgcacctt caacgttgta
    62281 gctggcacga tagtttttgg tcattttgtt gccattctgc gcggtagcga tgatggcgat
    62341 atccgctttg gtcgcgatgt tgtagcgcac gttgccctgg gacacgtcag catacagttg
    62401 gctaacgatg atttgcagat taaccgggcc attcggacca accatgtaac cacgcgcggt
    62461 catctgtttt tccagcactt cttgcagcag gaaacgcaga tcgcgggagg cggtcagggt
    62521 aacgatttga ttatcgcggg tgacttttgc cagcgcctga tcggtacgct gatcggcacc
    62581 attaatgctt acggtgacgc ccatcaggct tggatcctgc tgtggcagtg taatcgtcgg
    62641 ggaaacttca atagttgttg gcggttttgc gcatcctgca agcataaaca gagcaactaa
    62701 cgggaagagg atttttttga acatgttcgg gctctcagag actcttaagc gtgtttggta
    62761 aaaattcccg ccatcataac attgccaacg gcgaggggaa gtgggtaagg catgtaaatt
    62821 catcatgttg acgaaataat cgcccctggt aaaagaaaca ctgatgcgag gcctgtgttt
    62881 caatctttaa atcagtaaac ttcatacgct tgacggaaaa accaggacga aacctaaata
    62941 tttgttgtta agctgcaatg gaaacggtaa aagcggctag tatttaaagg gatggatgac
    63001 atctcagcgt tgtcggagga gatatttcat gatgatacgt gagcggatag aagaaaaatt
    63061 aagggcggcg ttccaacccg tattcctcga agtagtggat gaaagctatc gtcacaatgt
    63121 cccagccggc tctgaaagcc attttaaagt tgtgctggtc agcgatcgtt ttacgggtga
    63181 acgttttctg aatcgtcatc gaatgattta cagtacttta gcggaggaac tctctactac
    63241 cgttcatgcg ctggctctgc atacttacac tattaaggag tgggaagggt tgcaggacac
    63301 cgtctttgcc tctcctccct gtcgtggagc aggaagcatc gcgtaaaaac gcatttgcaa
    63361 ctgtcggcgc ttttccagta tgttgctaaa gattttatga aaaacggcct gcgggccgtt
    63421 ttgttttgtc tggattttgc gctttttgcc cagcattcag acgaaaattg cccgggaatt
    63481 gtgaaaaaat acgcgacagc gcgcaataac cgttctcgac tcataaaagt gatgccgcta
    63541 taatgccgcg tcctatttga atgctttcgg gatgattctg gtaacaggga atgtgattga
    63601 ttataagaac atcccggttc cgcgaagcca acaacctgtg cttgcggggt aagagttgac
    63661 cgagcactgt gattttttga ggtaacaaga tgcaagtttc agttgaaacc actcaaggcc
    63721 ttggccgccg tgtaacgatt actatcgctg ctgacagcat cgagaccgct gttaaaagcg
    63781 agctggtcaa cgttgcgaaa aaagtacgta ttgacggctt ccgcaaaggc aaagtgccaa
    63841 tgaatatcgt tgctcagcgt tatggcgcgt ctgtacgcca ggacgttctg ggtgacctga
    63901 tgagccgtaa cttcattgac gccatcatta aagaaaaaat caatccggct ggcgcaccga
    63961 cttatgttcc gggcgaatac aagctgggtg aagactttac ttactctgta gagtttgaag
    64021 tttatccgga agttgaactg cagggtctgg aagcgatcga agttgaaaaa ccgatcgttg
    64081 aagtgaccga cgctgacgtt gacggcatgc tggatactct gcgtaaacag caggcgacct
    64141 ggaaagaaaa agacggcgct gttgaagcag aagaccgcgt aaccatcgac ttcaccggtt
    64201 ctgtagacgg cgaagagttc gaaggcggta aagcgtctga tttcgtactg gcgatgggcc
    64261 agggtcgtat gatcccgggc tttgaagacg gtatcaaagg ccacaaagct ggcgaagagt
    64321 tcaccatcga cgtgaccttc ccggaagaat accacgcaga aaacctgaaa ggtaaagcag
    64381 cgaaattcgc tatcaacctg aagaaagttg aagagcgtga actgccggaa ctgactgcag
    64441 aattcatcaa acgtttcggc gttgaagatg gttccgtaga aggtctgcgc gctgaagtgc
    64501 gtaaaaacat ggagcgcgag ctgaagagcg ccatccgtaa ccgcgttaag tctcaggcga
    64561 tcgaaggtct ggtaaaagct aacgacatcg acgtaccggc tgcgctgatc gacagcgaaa
    64621 tcgacgttct gcgtcgccag gctgcacagc gtttcggtgg caacgaaaaa caagctctgg
    64681 aactgccgcg cgaactgttc gaagaacagg ctaaacgccg cgtagttgtt ggcctgctgc
    64741 tgggcgaagt tatccgcacc aacgagctga aagctgacga agagcgcgtg aaaggcctga
    64801 tcgaagagat ggcttctgcg tacgaagatc cgaaagaagt tatcgagttc tacagcaaaa
    64861 acaaagaact gatggacaac atgcgcaatg ttgctctgga agaacaggct gttgaagctg
    64921 tactggcgaa agcgaaagtg actgaaaaag aaaccacttt caacgagctg atgaaccagc
    64981 aggcgtaatt tacgcagcat aacgcgctaa attcgcacaa aggcccgtca ccgccaggtg
    65041 gtgggctttt ttttgtcatg aattttgcat ggaaccgtgc gaaaagcctc tttcggtgtt
    65101 agcgtaacaa caaaagattg ttatgcttga aatatggtga tgccgtaccc ataacacagg
    65161 gactagctga taatccgtcc ataaggttac aatcggtaca gcaggttttt tcaattttat
    65221 ccaggagacg gaaatgtcat acagcggcga acgagataac tttgcacccc atatggcgct
    65281 ggtgccgatg gtcattgaac agacctcacg cggtgagcgc tcttttgata tctattctcg
    65341 tctacttaag gaacgcgtca tttttctgac tggccaggtt gaagaccaca tggctaacct
    65401 gattgtggcg cagatgctgt tcctggaagc ggaaaaccca gaaaaagata tctatctgta
    65461 cattaactcc ccaggcgggg tgatcactgc cgggatgtct atctatgaca ccatgcagtt
    65521 tatcaagcct gatgtcagca ccatctgtat gggccaggcg gcctcgatgg gcgctttctt
    65581 gctgaccgca ggggcaaaag gtaaacgttt ttgcctgccg aattcgcgcg tgatgattca
    65641 ccaaccgttg ggcggctacc agggccaggc gaccgatatc gaaattcatg cccgtgaaat
    65701 tctgaaagtt aaagggcgca tgaatgaact tatggcgctt catacgggtc aatcattaga
    65761 acagattgaa cgtgataccg agcgcgatcg cttcctttcc gcccctgaag cggtggaata
    65821 cggtctggtc gattcgattc tgacccatcg taattgatgc cagaggcgca actgtgccgc
    65881 tatacttatc cagggcggca caacgctgta agcggcttgc gcctgagaat ggcatttgcg
    65941 tcgtcgtgtg cggcacaaag aacaaagaag aggttttgac ccatgacaga taaacgcaaa
    66001 gatggctcag gcaaattgct gtattgctct ttttgcggca aaagccagca tgaagtgcgc
    66061 aagctgattg ccggtccatc cgtgtatatc tgcgacgaat gtgttgattt atgtaacgac
    66121 atcattcgcg aagagattaa agaagttgca ccgcatcgtg aacgcagtgc gctaccgacg
    66181 ccgcatgaaa ttcgcaacca cctggacgat tacgttatcg gccaggaaca ggcgaaaaaa
    66241 gtgctggcgg tcgcggtata caaccattac aaacgtctgc gcaacggcga taccagcaat
    66301 ggcgtcgagt tgggcaaaag taacattctg ctgatcggtc cgaccggttc cggtaaaacg
    66361 ctgctggctg aaacgctggc gcgcctgctg gatgttccgt tcaccatggc cgacgcgact
    66421 acactgaccg aagccggtta tgtgggtgaa gacgttgaaa acatcattca gaagctgttg
    66481 cagaaatgcg actacgatgt ccagaaagca cagcgtggta ttgtctacat cgatgaaatc
    66541 gacaagattt ctcgtaagtc agacaacccg tccattaccc gagacgtttc cggtgaaggc
    66601 gtacagcagg cactgttgaa actgatcgaa ggtacggtag ctgctgttcc accgcaaggt
    66661 gggcgtaaac atccgcagca ggaattcttg caggttgata cctctaagat cctgtttatt
    66721 tgtggcggtg cgtttgccgg tctggataaa gtgatttccc atcgtgtaga aaccggctcc
    66781 ggcattggtt ttggcgcgac ggtaaaagcg aagtccgaca aagcaagcga aggcgagctg
    66841 ctggcgcagg ttgaaccgga agatctgatc aagtttggtc tgatccctga gttcattggt
    66901 cgtctgccgg ttgtcgcaac gttgaatgaa ctgagcgaag aagctctgat tcagatcctc
    66961 aaagagccga aaaacgccct gaccaagcag tatcaggcgc tgtttaatct ggaaggcgtg
    67021 gatctggaat tccgtgacga ggcgctggat gctatcgcta agaaagcgat ggcgcgtaaa
    67081 accggtgccc gtggcctgcg ttccatcgta gaagccgcac tgctcgatac catgtacgat
    67141 ctgccgtcca tggaagacgt cgaaaaagtg gttatcgacg agtcggtaat tgatggtcaa
    67201 agcaaaccgt tgctgattta tggcaagccg gaagcgcaac aggcatctgg tgaataatta
    67261 accattccca tacaattagt taaccaaaaa ggggggattt tatctcccct ttaatttttc
    67321 ctctattctc ggcgttgaat gtgggggaaa catccccata tactgacgta catgttaata
    67381 gatggcgtga agcacagtcg tgtcatctga ttacctggcg gaaattaaac taagagagag
    67441 ctctatgaat cctgagcgtt ctgaacgcat tgaaatcccc gtattgccgc tgcgcgatgt
    67501 ggtggtttat ccgcacatgg tcatcccctt atttgtcggg cgggaaaaat ctatccgttg
    67561 tctggaagcg gcgatggacc atgataaaaa aattatgctg gtcgcgcaga aagaagcttc
    67621 aacggatgag ccgggtgtaa acgatctttt caccgtcggg accgtggcct ctatattgca
    67681 gatgctgaag ctgcctgacg gcaccgtcaa agtgctggtc gaggggttac agcgcgcgcg
    67741 tatttctgcg ctctctgaca atggcgaaca cttttctgcg aaggcggagt atctggagtc
    67801 gccgaccatt gatgagcgag aacaggaagt gctggtgcgt actgcaatca gccagttcga
    67861 aggctacatc aagctgaaca aaaaaatccc accagaagtg ctgacgtcgt tgaatagcat
    67921 cgacgatccg gcgcgtctgg cggataccat tgctgcacat atgccgctga aactggctga
    67981 caaacagtcc gttctggaga tgtccgacgt taacgaacgt ctggaatatc tgatggcaat
    68041 gatggaatcg gaaatcgatc tgctgcaggt tgagaaacgc attcgcaacc gcgttaaaaa
    68101 gcagatggag aaatcccagc gtgagtacta tctgaacgag caaatgaaag ctattcagaa
    68161 agaactcggt gaaatggacg acgcgccgga cgaaaacgaa gccctgaagc gcaaaatcga
    68221 cgcggcgaag atgccgaaag aagcaaaaga gaaagcggaa gcagagttgc agaagctgaa
    68281 aatgatgtct ccgatgtcgg cagaagcgac cgtagtgcgt ggttatatcg actggatggt
    68341 acaggtaccg tggaatgcgc gcagcaaggt caaaaaagac ctgcgtcagg cgcaggaaat
    68401 ccttgatacc gaccattatg gtctggagcg cgtgaaagat cgcatccttg agtaccttgc
    68461 ggttcaaagc cgtgtcaaca aaatcaaggg accgatcctc tgcctggtag ggccgccggg
    68521 ggtaggtaaa acctctcttg gtcagtccat cgccaaagcc accgggcgta aatatgtccg
    68581 tatggcgctg ggcggcgtgc gtgatgaagc ggaaatccgt ggtcaccgcc gtacttacat
    68641 cggttctatg ccgggtaaac tgatccagaa aatggcgaaa gtgggcgtga aaaacccgct
    68701 gttcctgctc gatgagatcg acaaaatgtc ttctgacatg cgtggcgatc cggcctccgc
    68761 actgcttgaa gtgctggatc cagagcagaa cgtagcgttc agcgaccact acctggaagt
    68821 ggattacgat ctcagcgacg tgatgtttgt cgcgacgtcg aactccatga acattccggc
    68881 accgctgctg gatcgtatgg aagtgattcg cctctccggt tataccgaag atgaaaaact
    68941 gaacatcgcc aaacgtcacc tgctgccgaa gcagattgaa cgtaatgcac tgaaaaaagg
    69001 tgagctgacc gtcgacgata gcgccattat cggcattatt cgttactaca cccgtgaggc
    69061 gggcgtgcgt ggtctggagc gtgaaatctc caaactgtgc cgcaaagcgg ttaagcagtt
    69121 actgctcgat aagtcattaa aacatatcga aattaacggc gataacctgc atgactacct
    69181 cggtgttcag cgtttcgact atggtcgcgc tgataacgaa aaccgtgtcg gtcaggtaac
    69241 cggtctggcg tggacggaag tgggcggtga cttgctgacc attgaaaccg catgtgttcc
    69301 gggtaaaggc aaactgacct ataccggttc gctcggcgaa gtgatgcagg agtctattca
    69361 ggcggcgtta acggtggttc gtgcgcgtgc ggaaaaactg gggatcaacc ctgattttta
    69421 cgaaaaacgt gacatccacg tccacgtacc ggaaggtgcg acgccgaaag atggtccgag
    69481 tgccggtatt gctatgtgca ccgcgctggt ttcttgcctg accggtaacc cggttcgtgc
    69541 cgatgtggca atgaccggtg agatcactct gcgtggtcag gtactgccga tcggtggttt
    69601 gaaagaaaaa ctcctggcag cgcatcgcgg cgggattaaa acagtgctaa ttccgttcga
    69661 aaataaacgc gatctggaag agattcctga caacgtaatt gccgatctgg acattcatcc
    69721 tgtgaagcgc attgaggaag ttctgactct ggcgctgcaa aatgaaccgt ctggcatgca
    69781 ggttgtgact gcaaaatagt gacctcgcgc aaaatgcact aataaaaaca gggctggcag
    69841 gctaattcgg gcttgccagc ctttttttgt ctcgctaagt tagatggcgg atcgggcttg
    69901 cccttattaa ggggtgttgt aaggggatgg ctggcctgat ataactgctg cgcgttcgta
    69961 ccttgaagga ttcaagtgcg atataaatta taaagaggaa gagaagagtg aataaatctc
    70021 aattgatcga caagattgct gcaggggctg atatctctaa agctgcggct ggccgtgcgt
    70081 tagatgctat tattgcttcc gtaactgaat ctctgaaaga aggggatgat gtagcactgg
    70141 taggttttgg tacttttgcc gttaaagagc gtgctgcccg tactggccgc aacccgcaga
    70201 ccggtaaaga gatcaccatc gctgctgcta aagtaccgag cttccgtgca ggtaaagcac
    70261 tgaaagacgc ggtaaactaa gcgttgtccc cggtggggat gtgacgaagt tcaagggcgc
    70321 atctactgat gtgccttttt tatttgtatt cggtgacttt ctgcgtcttg tgggctgaca
    70381 attgcccccg tttcttgtca caataggcct ttgcgcgcat cgatacgttg cgtgaggtac
    70441 acagtcatct acagcggagt gttgttacac catgatggac agcttacgca cggctgcaaa
    70501 cagtctcgtg ctcaagatta ttttcggtat cattatcgtg tcgttcatat tgaccggcgt
    70561 gagtggttac ctgattggcg gaggcaataa ctacgccgca aaagtgaatg accaggaaat
    70621 cagccgtggg cagttcgaga acgcctttaa cagcgagcgt aatcgcatgc agcaacagct
    70681 gggcgatcaa tactccgagc tggcagcgaa cgaaggctat atgaaaaccc tgcgtcaaca
    70741 ggtgctgaat cgtctgatcg acgaggcgct gttggatcag tacgctcgtg agctgaaact
    70801 gggtatcagt gatgagcagg ttaaacaggc gattttcgcg accccagcct tccaggttga
    70861 cggcaaattt gataacagcc gctataacgg tatcctcaac cagatgggga tgaccgccga
    70921 tcagtacgcc caggcgctgc gtaaccagct cactacccaa cagctgatta acggtgttgc
    70981 cggtaccgat tttatgctga aaggtgaaac cgacgagctg gcggcactgg tcgcgcaaca
    71041 acgcgtggtg cgtgaggcga ctatcgatgt taacgcgctg gcggcgaagc agcctgtgac
    71101 cgaacaggag attgccagct actacgaaca aaacaaaaac aatttcatga cgccggaaca
    71161 attccgcgtg agttacatca agctggatgc cgcaacgatg cagcaaccgg ttagcgatgc
    71221 ggatatccag agctactacg atcagcatca ggatcaattc acccagccgc agcgtacccg
    71281 ctacagcatc atccagacca aaactgaaga tgaagcgaaa gcggtacttg atgagctgaa
    71341 taaaggcggt gattttgctg cattagccaa agaaaaatct gccgatatta tctctgcccg
    71401 taacggcggc gatatgggtt ggttagaaga tgccactatc cccgacgaac tgaaaaatgc
    71461 tggtctgaaa gaaaaagggc aactgtctgg tgtcatcaaa tcttcggtcg gtttcctgat
    71521 tgtacgtctg gacgacattc agccagcgaa agtgaaatcg ttagacgaag tacgtgacga
    71581 cattgcggcg aaagtgaaac acgaaaaagc cctcgatgcg tactatgcgc tgcagcagaa
    71641 agtgagcgat gcggcaagca acgacaccga gtctctggcc ggtgcagagc aaactgccgg
    71701 cgttaaagcc actcagacgg gttggttcag caaagataac ctgccggaag agttgaactt
    71761 caagccggtt gccgacgcta tctttaacgg cggtctggta ggtgaaaacg gcgcgccggg
    71821 catcaactct gacatcatca ccgtagacgg cgaccgcgca ttcgtgctgc gcatcagcga
    71881 gcacaaaccg gaagcggtga aaccgttggc agatgttcag gaacaagtta aggcattggt
    71941 tcagcacaac aaagctgaac aacaggcgaa agtggatgct gagaaactgc tggttgattt
    72001 gaaagccggc aaaggtgcgg aagctatgca ggctgccggt ctgaaatttg gcgagccgaa
    72061 aaccttaagc cgttctggtc gtgacccgat tagccaggcg gcgtttgcac tgccactgcc
    72121 agcgaaagac aaaccgagct acggtatggc gaccgatatg caaggtaatg tggttctgct
    72181 ggcgctggat gaagtgaaac aaggttcaat gccggaagat cagaaaaaag cgatggtgca
    72241 gggtatcacc cagaacaacg cacaaatcgt ctttgaagct ctgatgagta acctgcgtaa
    72301 agaggcgaaa atcaaaattg gcgatgcgct ggaacagcaa taatcctgaa gccgcctcgc
    72361 aaaaaaatgc gttgcagctg taacaagaaa aggtcgcttt cgcggccttt tccatttctg
    72421 aacattgcca tttgtttact gttttcactg ccgttaaggt gattccactg ttaacaaaca
    72481 aggagaaaac agtatgaaac acggaattaa agcactgctc attaccctgt ctctggcttg
    72541 tgccggaatg tctcatagcg cgctggcggc agctcctgcg gcgaaaccga caacggtaga
    72601 aaccaaagcg gaagctcctg cagcacaaag taaagcagca gtaccggcga aagccagtga
    72661 cgaagaaggc acccgggtca gcattaataa tgccagcgcg gaagagctag cccgcgcgat
    72721 gaatggcgtt ggcctgaaga aagcgcaggc gattgtcagt tatcgcgaag agtacggtcc
    72781 gtttaaaacg gtggaggatc taaagcaggt gccggggatg ggcaattcgc tggtggaacg
    72841 taatctggcg gtattaaccc tgtaattaat ttgcatagtg gcaatttttt gccagactga
    72901 agaggtcata ccagttatga cctctgtact tataacaaca acgtaaggtt attgcgctat
    72961 gcaaacacaa atcaaagttc gtggatatca tctcgacgtt taccagcacg tcaacaacgc
    73021 ccgctacctt gaatttctcg aagaagcccg ctgggatggg ttggaaaata gcgacagttt
    73081 tcagtggatg acggcccata acatcgcctt cgtcgtggtc aatatcaata ttaactatcg
    73141 tcgcccagcg gtattaagtg acctgttaac tattaccagt cagttgcagc aattaaacgg
    73201 taaaagcggc atcttaagcc aggtcattac actggagccg gaagggcagg tggtagcgga
    73261 tgcgcttatt acgtttgttt gtattgatct taaaacgcag aaagcattag ctctggaagg
    73321 ggaattgcgc gaaaagctgg agcagatggt taagtaaacg ttttgtggtg ccggatgctc
    73381 aagccgcatc cggcgacacc cggaataatt acctcaaccc ggttttctgc ttcatcgctg
    73441 ccatcaccgt cggtttatcg gccagataat gattcaaacc gttggcgcgt aaattacatg
    73501 ccgcacaatg accgcaaccg tcgcctttaa tgccgttata gcaggtcaac gtttcgttac
    73561 ggactaaatc cagtttgccg taataatctg ccagcgccca ggtttccgct ttatcaatcc
    73621 acatcagcgg cgtttcaaaa cgaatatctt tcgccatgcc caaactgacg gcatggttta
    73681 gtgctttcac aaactcatcg cggcaatccg ggtagccgga gaaatccgtt tcacagacgc
    73741 cggtaattac ggcttctgct tttacctgat acgcatatat tgccgccagc gtcaggaaca
    73801 aaatattacg ccctgggaca aacgtattcg ggatgccatc ggcttcaggt tcataatcag
    73861 gcaccggaat gctgtcacgt gtcaggctgc tgaccgccag ctcgttgagc agcgttacat
    73921 caagaacctt atgcgcgcgt gctcccagtt tcagcgccag ttcgcgtgcc acgtcgattt
    73981 ctgcgcgatg tcgctgaccg taatcgaacg tcacgcaatg gacttcatca tattgttgta
    74041 atgcctgcac cagacaggtg gtggaatcct ggcctccact gaacacaacg acagcacgtt
    74101 tcatagataa tcctgcttga acaataaaag cgttatggta acgcctgcga ttaacccgga
    74161 ccagctattc attgcgacgg cgcgggaagc caggcttcgg taaattcaaa ccagccgcgc
    74221 ggtgtcagtc gcacaccgtt cacgccggga ggggcactaa tgcgatagtg atagttgaac
    74281 agcggcgtca gcgtcgcgtc tgccattaac tggctaaaaa ccgctttcag ggcattaaat
    74341 cggttttcct catcaggcat tacttgcacc gcgtccagcg tcgattgcag atgtgcatat
    74401 gctggagcgt cgaaaacatg tggccatagt ggatcgcaac gtagccattg ctccagagta
    74461 tattccggtg cttcgccaat taatctgtcg cccatcatga ggtctgcgtg tgccagtagg
    74521 gtcgtgtcgt cccagttttt tgcgttatga aaaataattg tgagttcaca gccttccgct
    74581 gccagtgtcg cctgtaggcg ttctgccatg gtatgaagtt ctatcggtaa gtgataaacc
    74641 agcgtcaagg ttttcggtag tttgacttca tccggtactt gccagtgcgg aatagtccag
    74701 cctggcagta atgcatgact ggcggtgatc aggttttctc cgacttctaa cgtttgtaat
    74761 aaaccggatt gatgaataat ggagatcact tttcgcgcct gccagaggga gagtcgggga
    74821 cttttgcgca acgttaaata gcaaaaacct aaactgatgc cactactgac ctggctgacc
    74881 cgttgcagct cctccggttt gccgatggtg atttgcacgg gatgccgaca actggttccc
    74941 aaatcttttt cgaaaagcgg cggagttatc cagtactcaa ccgctttaag cagcggatga
    75001 cgcaaatggt aataatcatg gctctccagg cgcaccagct ccgctgtgaa ttgtgttaag
    75061 cgaaaaggac cagtgccgat cagtgggaat tgcggatgcg ccagatggct gcaatagctc
    75121 gccagccggt gcgcaagcca gtaatcgggg cgatgtaaaa agaaggtcag acactgcgga
    75181 tgggtgactt caatacgctt cacgctaata aataattgat ccagtgctgg cagttgtaac
    75241 agcatcaata atcgctggtg taagtgtgag gcttttactg catcgccgtt atgccagtgt
    75301 agggttgaac gaagataaaa gtcccagcgt aacccgtcag tagaggtttc ccagtgatgc
    75361 gctaaatcgc caatcgggcg ctgagtatta ttatcgaagc gggtcaggcc ggaaaatatc
    75421 tgcccggcga gatgctgctc ggcacggccg ggcaaaaagc ctggttgtag cggttcgagc
    75481 gggcgatagt agggaatacg caacgtgggt gtatcgtttt gccattgtcc gcccataaac
    75541 ggctgtaaca gagtgcgcag ctcacctggg gccagttgcg ccagctccag cacatcttgc
    75601 tgctttccgg tttccagtgc ctgttccatc atcgcattgc gtagcgattc cggcgtgacc
    75661 agaaagcgta attgtccgcg ctttccgcgt cctgactgcg cctgccactc cagccatccc
    75721 gcctcctgtg cctgacgcaa cagcgtacga acatggcgtt cgctgcaaaa acagcgttcg
    75781 gccagttcgc tgacggtgac ggtttgcggc tttccggctg aaggttgcca cagacgttga
    75841 tactggttaa gacggttgag caatcgcata taaacccgga acaatattat ttaactattc
    75901 actattactt ccgtatatat caggtgatac tcaatcacca ttaaccgtgt cacagagtgg
    75961 agaaagaaat ggctcgtctg gcagcatttg atatggatgg cactttattg atgcctgacc
    76021 atcatttagg tgagaaaacc ctctctactt tggcgcgact gcgtgaacgc gacattaccc
    76081 tcacttttgc cacggggcgt catgcgctgg agatgcagca tattctcggg gcgctatcgc
    76141 tggatgcata tttgattacc ggcaacggaa cgcgcgtgca ttctctggaa ggtgaacttt
    76201 tacatcgtga tgatttacct gcggatgtcg cggagctggt gctgtatcag caatgggata
    76261 cccgagccag catgcatatc ttcaatgacg acggttggtt taccgggaaa gagatccctg
    76321 cgttgttgca ggcatttgtc tatagcggtt ttcgttatca gataattgat gtcaaaaaaa
    76381 tgccactcgg cagcgtcacc aagatctgct tctgtggcga tcacgacgat cttacacgct
    76441 tgcagatcca gctatacgaa gcattaggcg agcgtgcaca tttgtgtttt tccgccacgg
    76501 attgcctcga agtgctgccg gtgggctgca ataaaggcgc tgcattgacg gtgctgaccc
    76561 aacatttagg tttatcgttg cgcgattgca tggcctttgg tgatgcgatg aacgatcgcg
    76621 aaatgttagg cagcgtcggt agcggcttta ttatgggcaa tgcgatgccg caactgcgcg
    76681 cggagctccc gcatttaccg gtgattggac attgccgaaa tcaggctgtc tctcactatt
    76741 tgacgcactg gctggactat ccacatctac cttattcccc cgaataacga gatcccttcc
    76801 agcaccgggc aattgcccgg ttttttttgc gttgaatttg tcattttgtg ccgtggtgtt
    76861 taaaccgcac agaataaatt gtcgcgattt cacctttaaa atagaattaa aagagaaaaa
    76921 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
    76981 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
    77041 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
    77101 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
    77161 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
    77221 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
    77281 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
    77341 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
    77401 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
    77461 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
    77521 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
    77581 actaccgggc agatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
    77641 ctcctgcgtt acgtctggcg ggtattgctg tttggtgcgt cttatcaact ggctgttgaa
    77701 ctgcgtgaag attattaccg tcagctaagc cggcagcatc ctgagtttta cctgcgtcat
    77761 cgcacgggtg acctcatggc tcgtgcgact aatgacgtcg atcgcgtcgt gtttgccgcc
    77821 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
    77881 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttaccgat gccagtgatg
    77941 gcgatcatga ttaagcgcaa cggcgatgcc ttgcatgaac gctttaagct ggcacaggcg
    78001 gcgttttcta gtcttaatga ccgcacccag gaaagcctca ccagtattcg catgatcaaa
    78061 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
    78121 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
    78181 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
    78241 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
    78301 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
    78361 cgcgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
    78421 cgtggcgaac tggatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
    78481 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatttg cgggccgact
    78541 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt cagcgagggg
    78601 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
    78661 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcgaa taacatcgcg
    78721 ctgggttgcc cgaatgccac ccagcaagag attgaacacg tagcgcggtt agccagcgta
    78781 catgacgata ttttgcgtct gccgcaaggt tacgatacag aagtgggcga gcgcggcgtg
    78841 atgctttccg gtgggcaaaa acagcgtatc tccattgctc gagcgttatt agtcaacgcg
    78901 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
    78961 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
    79021 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
    79081 ggcaatcatg atgagctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
    79141 caactggagg cggcgctcga cgacgttccg gaaattcgcg aggaggccgt cgatgcgtag
    79201 ttttagccaa ctgtggccga ctctcaagcg tctgttagcg tacggttcgc cgtggcggaa
    79261 accgctgggg attgcggtcc tgatgatgtg ggttgcggca gcggcagaag tcagtgggcc
    79321 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaaat
    79381 ggttgcaggg ctggcggcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
    79441 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
    79501 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
    79561 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
    79621 ggcaactgtc ctgcgtagcg ccgcactggt gggcgcgatg ctggtggcaa tgttcagcct
    79681 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
    79741 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
    79801 caacgacggc tttaacgaaa tcatcaatgg catgagcgtt atccagcagt tccgtcagca
    79861 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
    79921 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
    79981 tctttgtggc ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
    80041 gttatatgcg tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
    80101 gcagcaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
    80161 cggaccgcgc cagcaatatg gcaatgatga tcgcccgtta cagagcggca ccatcgaagt
    80221 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
    80281 tgtgccttcg cgtaactttg tggcgctggt cgggcatacc ggcagtggca aaagcaccct
    80341 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
    80401 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
    80461 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctgggacgtg atatctccga
    80521 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gcagcatgag
    80581 tgatggtatt tatacgccgc tgggcgagca gggaaataat ctctcagtcg ggcaaaagca
    80641 actgctggca ctggcgcgcg tgctggtgga gacgccgcaa atcctgatcc ttgatgaggc
    80701 aaccgccagc attgactccg gtactgaaca ggcgattcaa catgctctgg cggcggtgcg
    80761 tgaacatacc acgctggtgg tgattgctca ccgcttatca actattgttg atgccgacac
    80821 cattctggtg cttcatcgtg gacaagccgt ggagcagggc actcaccagc aactgctggc
    80881 ggcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
    80941 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
    81001 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaatct tcttaacttc
    81061 ctgctctctt tctcattttt catttctggc acaccgcttg caatatcttc ttcgtgtagc
    81121 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
    81181 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
    81241 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
    81301 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
    81361 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
    81421 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
    81481 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
    81541 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
    81601 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
    81661 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
    81721 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
    81781 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
    81841 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
    81901 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
    81961 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
    82021 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
    82081 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
    82141 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcactggt
    82201 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
    82261 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
    82321 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
    82381 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
    82441 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
    82501 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
    82561 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    82621 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    82681 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggcctacgt
    82741 gtgccggaag aacaagagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    82801 aacgcgtaac aagcactgca aaaaatagcc ggacggtttt cacctccggc tattttttta
    82861 attgtgatta cgcattaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgggt
    82921 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    82981 cagcagccat tcattcaaat taaacgggcg atggaaccac atggaatggt caatggtggc
    83041 aatctgaatc cctggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    83101 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    83161 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    83221 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttaagcac
    83281 tggcggcagc aggtgcgcca gcgattgggc gatttgcgtt tccgaaggca ggccatcagg
    83341 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttctggtg cctggaaaga
    83401 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    83461 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    83521 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    83581 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    83641 actctggccg cgaaagagtc cttcctcaat tttttctaga tttaacaatg tcagtaaatt
    83701 ttttagcgcc tgactcatat aactctccag taacaaagct gccgcagcaa gccaaagtga
    83761 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatggtctga attacgggct
    83821 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    83881 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga aatgaatgaa
    83941 actcgtgcac atggccagtg gtttagcggt tgcgattgca ttggcggctt gcgcagataa
    84001 aagcgcggat attcagacgc cagccccggc tgcaaatacg tctatttcag caacacaaca
    84061 acctgctatc cagcaaccga atgtctccgg taccgtctgg atccgtcaga aagtcgcact
    84121 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcaccgtc
    84181 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    84241 tctgccattt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    84301 taccgtgaat gacaaactgg tatttatcac cgataccgtt cagccggtga tcaaccaggg
    84361 cggaactaaa gccgacctga cattggtgcc ggtacagcaa accgccgtgc cggttcaggc
    84421 cagcggtggc gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    84481 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    84541 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc attacacctt
    84601 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    84661 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    84721 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    84781 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    84841 ctttttccat ctttttttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    84901 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    84961 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    85021 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    85081 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    85141 tcaaaaaacc ccaacatggc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    85201 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    85261 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    85321 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    85381 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    85441 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    85501 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    85561 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    85621 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    85681 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    85741 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    85801 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    85861 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    85921 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    85981 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgatagt gattgatcat
    86041 ttcacgcagc aattgcggga ttttttccga ggtgagcacg gtggattcaa gattgatcga
    86101 aatatgctgc tgtggatgct gacgcagcca gtcgcccata tcttcaaaga cgcttcttat
    86161 aatcagtagc gtcaatggct cagaaaggcc cgtttgctgt gccagcggaa taaaactatc
    86221 tggtgacaac caactaccgt ctgtctgcgg ccagcgcgcc agtgcctcag caccgacaat
    86281 tttgccattg gctaaggaga caatcggctg atagtgcacg caaatatcac gattttcgat
    86341 agcatcctgc agccgatgat gcggtgactg gatacggcgc aaaatacgca gcacaaacat
    86401 cgctgccagc aggccaatca ccaacccgag cggtaaccaa ataaagactt gccgatgcca
    86461 gcctttctgc aacattttcg ttgaagccca ggtgatgatc gaaatattca tctccgggaa
    86521 gggcaggata tcgtaaatga ttccattatt ttcgatatgc tcaccgggtg ttttttgtag
    86581 cctggtaata attccctgag caatttcatc gctgctggtt atgacaacgt tatgggcatt
    86641 gccaataatg gcggcatcaa tttgccatga gctataggga atgacatcaa taaaggaagc
    86701 ggggtcgatc atgacgacat aatgtgccgt tcccatggcg accatgtaac ggataatgcc
    86761 taaatcgtta tgcgatgtta accagacacg ataaccatct ttcgaaattt tacctggctc
    86821 ggggaaggta tcgggcggac tttcatgctc cagagacgaa cactggggaa cgttgttatc
    86881 gatataaacc acttcctgaa tatagcgata actgtaagat acccgacgca tttccatgag
    86941 atgggcttcg ctacaggcag cgccttgcca tctttccagc tcttgcagcg catctttccc
    87001 ttgtgtcgcc accttattgg cccgaatagc gacgcgggag gaataggtat ccagctcttc
    87061 aataaacgat gtttctacct gctgatgggc cagccagatg cttaagccga caggcagcaa
    87121 tactgaaaga atcagtactc ccgaaataag gccgaccaga tgtcgtgttc tcaccagcat
    87181 atccttatta acacagtgta gggaaagtat atctgattgt ggaaaaaggc tggcaggaga
    87241 taaggaggta gagctggtat ttcatgaagt gatgggtgga aacgatcatt gtcatttgac
    87301 caggcgcaga gctttacgcc tggtatgtaa ccctaactta agatgttgat tcggcgcgag
    87361 cgagggtaaa aatatcgtaa aaagacagtt cacctttacg gacgatcatt ttttgcagtt
    87421 gttccttatg aacatccgcg acacgcggag agttcaagat ggcttcaacc agcgcggcag
    87481 gtacaaagcg cgtgttgtac cattcgccgt tatagcaaac gcggaaatcc agcacgtcga
    87541 tatcttcata gcggtagcgt gggtagacat cgaaaaagaa gaaaccatta agcaggataa
    87601 ataatacaac cagtagccag acagagcctg aaagcgtttc ggactgcaac atcaccgcca
    87661 gggtggcaaa aaaagcaacg tacataccga taaacagccc cggatgttta cggataaaac
    87721 tgatactaaa gcggggtttg ttgtcgcgtt tttcgcgggt attcagagac tcaatcgttt
    87781 cggtcagcag gcgttgtatt tcggtcatcg tttgccttcg tggtatctgc aactttccag
    87841 ggaacaccct attttaacgt gggaatagta gaggggaagt aacagattta ccacaatttt
    87901 gcataacagt tgcgaaaaac cgattacaat tttttaatta ttctggctgg attaccgccc
    87961 acgacaacgt tgttcgggac atcttttgtg acaactgcac ctgaggctac cacgacgtta
    88021 tcaccaatgg tcacaccagg gttaatgacc gcgcgtccgc caatccagac gttattaccg
    88081 atggtgacgg gtttccccag ttcagcaccg ctattacgtg ctacagggtc gatgggatgt
    88141 gttgccgtgt agatatgaac gcctggtgcc aacatacagt tatcaccgat gcgaataggc
    88201 agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attaccgaga aaaatgttat
    88261 agccatagtc acagcgaaac gttggctcaa tataagcctc tgtcacctga ccgaatagat
    88321 cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg tatcggtgaa
    88381 taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag cgatacaact
    88441 caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg ctgaaaatgc
    88501 ggcaacacag taagacgaaa agtcagtgaa gagaacgttc ccattacatc tgtgtgataa
    88561 agatcacata gggtatgtct tcatggcgaa aaagtataaa attcttaata aacagccggt
    88621 tatagctccg aaagcgaatt atttattagc gaataaattt ccaaactgag gaagggatct
    88681 tgtcgtacag tttattcatg gtcaattcgg cgaggcggtg atctgcggct gagtaaaata
    88741 ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc tccagcgtgt
    88801 caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa ggtttttcgg
    88861 acatacttct acccatggtt gtaataattc taacaagata aactcgcagg attctctttc
    88921 gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt ctgaagatcc
    88981 tgcatattaa taccataact actgaacaac ataaaggtgt catccagata ttcgtcgatc
    89041 tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc gaaggtcgca
    89101 atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc gttaacccag
    89161 ccatgattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc acagagaaac
    89221 ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg tccccttctt
    89281 agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct ggacttgccc
    89341 tctcgtttgt tagcttaact ataagccact ctttgcaggt tttcatcgca ttattacgaa
    89401 aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc actaacccgc
    89461 agcaggtaaa agcagtttgc tcgaactgtt cataacattt caggattatc gccagatgaa
    89521 attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag gaatgaataa
    89581 agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt aaatctatta
    89641 gcgctactta atataaatta actaacaatc agtatttttt atgaatttta tccgtggtta
    89701 atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa tgttcgtagg
    89761 ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat caatgatgat
    89821 cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg cgaaccacaa
    89881 caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc ccgcccatta
    89941 caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata accagcggca
    90001 taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc atccgcactg
    90061 catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct ttggcgaatt
    90121 cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg cctacctgga
    90181 agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg ataaccccca
    90241 gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc gccgccagac
    90301 acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg ccggagaggc
    90361 gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt ttgctcgcca
    90421 gttgttccat tagctccatt gcttcaccgg tacttttacc cggtgccgcc tggcctaaga
    90481 tttccatgga tggcaggcca ttgtaacgtt ccagacgcgg cgaaccgtac tcccaacgag
    90541 aagaggagaa tgccgagaac ggcaccatct gaccatcagc agcacgaaca taccagtcgc
    90601 cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact ttcttcacac
    90661 gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc agagtggtgt
    90721 taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata tcaatcttaa
    90781 actgcggggt atcttccaga ccgtttggac gtacgctggt cagcatatca gggtgcttcg
    90841 ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca aggccagcct
    90901 ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt gcgggcaggt
    90961 taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca cgcatggtaa
    91021 tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag gaaacgaacg
    91081 caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg aacaccgact
    91141 caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc actttctgcg
    91201 tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg ccctggtcct
    91261 catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg accacgatga
    91321 tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg ctgtcggtgt
    91381 agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct tttttacctt
    91441 ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc ggagtcagga
    91501 tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag aactgacgat
    91561 agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgcc gacagtacca
    91621 tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta gcttcttttg
    91681 gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg atagcgtcat
    91741 ccaccaacaa gccgatagcg agcaccatcc cgaacattgt tagcgtgttt atcgagaagc
    91801 caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca atggtcggaa
    91861 tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg aggatgatcg
    91921 cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc gtggtgtcgt
    91981 acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc agttcagcac
    92041 ggattgccgc agcggtatcc agcgcgtttg caccggtcgc cagcttgatc cccagaccgg
    92101 aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc agctcaatct
    92161 tcgccacatc acgcagcagt acgcgggaac catcttgatt cactttcagc aggattttgc
    92221 cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta agctgttggc
    92281 ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc tgcgctttga
    92341 tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc gggttcatcc
    92401 agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc gacgtacggc
    92461 tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc gtcatggtgc
    92521 catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc tcaacgctca
    92581 ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt ttgttctgca
    92641 cctgaacctg cgcgatgtcc gcatcagtac cagactcaaa ggtcagggtg atctgcacgg
    92701 tacccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg ttcatattct
    92761 gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg gggtaggagg
    92821 cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc agtttgagga
    92881 tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa atcgggcgat
    92941 cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt ggactgttca
    93001 ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg tgcttttacc
    93061 tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc tgctttcaga
    93121 ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg acgggtttcc
    93181 actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt acgggttacg
    93241 ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg tgcgcgcacg
    93301 aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag ggtgatagac
    93361 ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg cgggaactta
    93421 atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt cagcgtgcca
    93481 ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac atcaacgtag
    93541 atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg taccaatgcg
    93601 ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagaggtgac tttggtgtaa
    93661 gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc agcattcgcc
    93721 tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg agtaccgagc
    93781 agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg ggctttcgcc
    93841 agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat ctgatagaga
    93901 gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat aatcccgcta
    93961 acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag ctcggttgtg
    94021 atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg ctggccacct
    94081 tgttgggcct gtttgtcgtc acatcctgtt agggctaagc tgcctgagag catcagaacg
    94141 accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt gtccgatttc
    94201 aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta tcgtcgtgct
    94261 atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac gaacatatgg
    94321 cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat gtggctctac
    94381 gtcttttctc acagcagggg gtatcatcca cctcgctggg cgagattgca aaagcagctg
    94441 gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg ttcagtgaga
    94501 tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag gcaaaattcc
    94561 ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa tccacggtga
    94621 cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa tttgtcggag
    94681 aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat gaccgtatag
    94741 aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta atgacgcgtc
    94801 gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg ctctttgccc
    94861 cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta ctggagatgt
    94921 atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga atctgactcc
    94981 aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg tgatattctt
    95041 gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat catgactatg
    95101 ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc ttttgttttt
    95161 gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga tctgccgaca
    95221 aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct ttctgctcag
    95281 gacaaactgg tgcagcagga tctgacagat acattagcca ccctcgataa aatcgatcgc
    95341 ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga aaaaatgcgc
    95401 caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga aacgcgcaaa
    95461 attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc gctggacgat
    95521 ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc gttacagacg
    95581 cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca acaaattcgc
    95641 agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca gaaagtgtta
    95701 atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa aagcctggaa
    95761 gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac ggcgaacagc
    95821 gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa gcgcctgact
    95881 ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg tattcaggct
    95941 aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg tctgattacc
    96001 gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa ctggctggag
    96061 cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa gggcagcctg
    96121 ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga tgaactggaa
    96181 aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa ccagcagcgt
    96241 gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca caccaacgaa
    96301 gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg cgaattgctg
    96361 gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct gcaaatcaac
    96421 cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca gcaaatcttt
    96481 tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc gcaaagcctg
    96541 aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg gcctgccgtt
    96601 tttatcgctt tcctcgccgg tttgccgctg ctgttgattg ccgggctgat ccactggcgt
    96661 ctgggctggc tgaaagcgta tcaacagaaa ctggcttccg ctgtgggttc cctgcgtaac
    96721 gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc gctgccggtg
    96781 tgcctgatta ttctcgcggt tggcctgatt ctgttgacca tgcagctcaa catcagcgaa
    96841 ctgctatggt cgttcagtaa aaaactggcg attttctggc tggtgtttgg cctgtgctgg
    96901 aaggtactgg agaaaaatgg cgttgccgtg cgtcacttcg gcatgccgga gcagcagacc
    96961 agccactggc gtcggcaaat tgtccgcatc agtctcgcgt tgctgcctat ccatttctgg
    97021 tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg gcaggcgatg
    97081 attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg cgaaagctgg
    97141 cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc gattatcccg
    97201 attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg cctctccggg
    97261 cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca gacggtattg
    97321 cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc gcgtcggcag
    97381 aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac cattgcactg
    97441 gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc gctgttcggt
    97501 gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatct cgacagcatc
    97561 acgctctggc attacaacgg cactgaagct ggcgctgcgg tggtgaaaaa cgtcaccatg
    97621 ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat tcgcaacctg
    97681 cctggtttac tggaagtgct ggtgctctcg cgactgaata tgcgtcaggg cgcgtcgtat
    97741 gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac ggtgttcgga
    97801 tcgctgggcg tctcttggga taaactccag tggctggccg cagcattatc cgtaggtctt
    97861 ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat tctgttcgaa
    97921 cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac ggtaagtaag
    97981 atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat catcccgaac
    98041 aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac tacgcgtctg
    98101 gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa agtgttactg
    98161 aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt cttctttacg
    98221 gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga actgcgtgac
    98281 cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg tgaaaacgac
    98341 atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg cgatgaggtg
    98401 acggaagtca aacgcgacta caaaggcgat gacccgacgc cagcggtagg gtaataacca
    98461 aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt tcatgccgga
    98521 tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta ttgcaggagc
    98581 ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac aatcttaggg
    98641 gacatttagc ggccccattt atttctcact tttccgcctc atcatcgcgc gttaatttct
    98701 tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct gggatctgat
    98761 tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg gcattttcca
    98821 gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc gattttttcc
    98881 agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg atgcagcgtt
    98941 tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgctcggc aaccatctca
    99001 cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg ggcaattttc
    99061 ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc gatggcttcc
    99121 agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg ctgctcaact
    99181 gcatgacgaa gcgcagccag attatcgccc gcctcatcga gacaagcctg cagtgttgtc
    99241 gcacgggttt gaaaaaggtg tctgtcaaaa cgggcactta gcgtggcaaa ttgcgctacc
    99301 ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag cagcagggcg
    99361 gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag ccactctgta
    99421 agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg ctggcggtag
    99481 tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt atcctgctgg
    99541 cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg taccgctcat
    99601 ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc ggcgtcaaac
    99661 cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc gtccagatgc
    99721 catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat atgtggcgaa
    99781 ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca gttgcaatta
    99841 ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt caaaggttaa
    99901 acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa caagcaggca
    99961 tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca tcaaaagcat
   100021 tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac tggaagaccc
   100081 gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg cgggcattac
   100141 caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag ctctgggtct
   100201 gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa ccatcagtga
   100261 aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg ccatcaaacc
   100321 gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta tcgaagcgac
   100381 cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca ttatcaacct
   100441 gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct acagccttgt
   100501 cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg gacagcacaa
   100561 gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc ttcgtgaatc
   100621 caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg aaaatggcgc
   100681 ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact ggcgaacggc
   100741 ttgtcgttag ggcgtattca tcatgcttat cttttttccg gtacccgtgg cgtcggaaaa
   100801 acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat taccgcgacg
   100861 ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt cgatctgatt
   100921 gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct ggataacgtc
   100981 cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt gcatatgctg
   101041 tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga gcacgttaag
   101101 tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc gcgctgtttg
   101161 caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga gcacatcctc
   101221 aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg cgccgctgaa
   101281 ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg tgacggccag
   101341 gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg acgacgatca ggcgctgtcg
   101401 ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat taatgaagcc
   101461 gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct gttgcatcgt
   101521 attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc cattgagctg
   101581 cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta ctatcagacg
   101641 ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg cgttgagatg
   101701 acgctgctgc gcgcgctggc gttccatcca cgtatgcctc tgcctgagcc agaagtgcca
   101761 cgccagtcct ttgcgcccgt cgcgccaacg gcagtaatga cgccaaccca ggtgccgccg
   101821 caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac caccagccag
   101881 gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc aaaaaagagt
   101941 gaaccggcag ccgctacccg cgcgcggccg gtgaataacg ctgcgctgga aagactggct
   102001 tcggtcaccg atcgcgttca ggcgcgtccg gtgccatcgg cgctggaaaa agcgccagcc
   102061 aaaaaagaag cgtatcgctg gaaggctacc actccagtga tgcagcaaaa agaagtggtc
   102121 gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga actggcggcg
   102181 aagctggcgg cagaagccat tgagcgcaac gcgtgggcgg cacaggtgag tcaactttcg
   102241 ctaccaaaac tggtcgaaca ggtggcctta aatgcctgga aagaggagag cgacaacgca
   102301 gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc acagcaaaaa
   102361 ctggctgaag cgttgagcac gttaaaaggt tcaacggttg aactgactat cgttgaagat
   102421 gataatcccg cggtgcgtac gccgctggag tggcgtcagg cgatatacga agaaaaactt
   102481 gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg tcggttcttc
   102541 gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca gcttacgttc
   102601 gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc tgggtaacct
   102661 gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag agatcgcgca
   102721 gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca acggtgcaca
   102781 caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag agatgctgga
   102841 agacctggtg gctgcagcat ttaacgacgc agcacgtcgt attgaagaaa cgcagaaaga
   102901 aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga tgccgttctg
   102961 atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct gccgggcgtt
   103021 ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg tagcggcggg
   103081 atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg cgccgattgc
   103141 cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg tcaggaaaac
   103201 ggtcaaatct gtgtggtgga gagtccggcg gacatctacg ccattgagca gacggggcag
   103261 ttttcaggcc gttattttgt gttgatgggg cacctgtcac cgctggacgg catcggtccg
   103321 gatgatatcg gccttgatcg tctggaacag cgtctggcag aggaaaaaat cactgaagtg
   103381 atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat tgccgagctt
   103441 tgcgcgcaat atgacgtgga agccagccga atcgctcatg gcgtaccggt tggcggcgaa
   103501 ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca taagattcgt
   103561 ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc ccttgtcccc
   103621 atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga cctacatgaa
   103681 aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc tgatgatcca
   103741 ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg cctccgatgc
   103801 ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag gtgatggcga
   103861 actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct ccgataacgg
   103921 cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta aatccggtac
   103981 caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc tgatcggtca
   104041 gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgaccg tgcgtactcg
   104101 cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa tcggctggcg aaggtgaata
   104161 taccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc atctgcgtga
   104221 aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca aatactccga
   104281 ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg aaaccgttat
   104341 ctcctgggag aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt cggaaatcac
   104401 cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaatg atccgctgac
   104461 ctggagccac aaccgcgttg aaggtaagca ggagtacacc agcctgctgt acatcccgtc
   104521 ccaggctccg tgggatatgt ggaaccgtga tcataaacac ggcctgaaac tgtacgtcca
   104581 gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc gcttcgtgcg
   104641 tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc tccaggacag
   104701 cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa tgctggaaaa
   104761 actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg gcctggtact
   104821 gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac tgctgcgttt
   104881 tgcttctact tataccgatt cttctgcgca gaccgtgtct ctggaagact acgtttcccg
   104941 catgaaagaa gggcaggaga aaatctacta catcactgca gacagctatg cggcagcgaa
   105001 gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc tgctttccga
   105061 ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac cgttccagtc
   105121 ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg agagcgcgaa
   105181 agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccctgc tcggcgagcg
   105241 cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg tttcgaccga
   105301 cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc agaaagtgcc
   105361 agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac gtgcggcaga
   105421 tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc aggcgctgct
   105481 ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga accagctgct
   105541 ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg gcatttttct tttcatcatc
   105601 tgcactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc tttcttgagg
   105661 caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac attttaaggg
   105721 gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag ggactcaggc
   105781 tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata tgctgcgtgc
   105841 tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg atgctggcaa
   105901 actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc aggaagactg
   105961 ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag acgcgatgaa
   106021 agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg aactgattgt
   106081 tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc acgttaaatt
   106141 caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga ctacccgtaa
   106201 agacgatcag gaagaaaccg tgcgtaaacg tctggttgaa taccatcaga tgactgcacc
   106261 gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg cgaaagttga
   106321 cggcaccaag ccggttgctg aagttcgcgc tgatctagaa aaaatcctcg gctaattcga
   106381 aagcgcgcac ggacagtccc ctcgccccct cggggagagg gttagggtga ggggaacagg
   106441 cccgcacaag caaacttatc agcaatctca ggccggatat tcattcggcc ttttacaaaa
   106501 acctcaatct gcgctattcc tttttctgat ttgacctctc acagcaatta gttcttcttc
   106561 ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaga
   106621 ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca cctgaagcgg
   106681 taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt
   106741 tatggtggcc gttgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt gtggcgaagc
   106801 tgtacgcctc ggtctggatg gaagacggct cgccgctgat ggtttacagc cgccagcaac
   106861 agcaggcgtt ggcacaacgt ttaccggata cgcccgtagc gctgggcatg agctacggct
   106921 cgccatcact ggaaagcgcc gtcgatgaac tcctggcaga gcatgtagat catattgtgg
   106981 tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg gatgaactgg
   107041 cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg
   107101 ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt gccaaacatg
   107161 gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat gcagatgaag
   107221 gcgatgatta cccgcaacgt tgccgcacaa cgactcgcga actggcttcc gcactgggga
   107281 tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga
   107341 tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt catatacagg
   107401 tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa
   107461 accgtgaggt cttcctcggt gccggcggga aaaaatatga atatattcca gcgcttaatg
   107521 ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc taaagctgag
   107581 cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc attcgtgaat
   107641 aatgcaaaaa ggcatgcagc gtacctgggt agagtttgaa ctcacagggc tgctgatgcg
   107701 ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac tccgccccgg
   107761 caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag taatacggtg
   107821 actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa tcctgttgcg
   107881 ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt aatccgtaaa
   107941 gcccatacca cagcaaaaca cccgcaattt taccgcaatc gatctgttta tcacgcaacc
   108001 acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag ccaatgcggg
   108061 acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca gccacaattt
   108121 cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggg gtaatcaata ccaatcaccg
   108181 tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg agattgccga
   108241 gaataaaacc gcctccatgc aaataaaata gcgtcgccgg gctatctggc tgcggacaaa
   108301 agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg gttgccattt
   108361 ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt tgctcagcaa
   108421 tcgttcccgt tgcgggccag gacggtaaat ccgactgaag agtattcaca acggtcttca
   108481 tttcagcaga aataaggtcc agaacaggta gtttgttttc cggcttcata caaaaactcc
   108541 tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg cctgctgata
   108601 ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg ccgctcagtt
   108661 aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa cattacttcc
   108721 ccgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa accagcgctg
   108781 cctgggtagt gggtatcgat caaacgctgg tcgatattga agcgaaagtg gatgatgaat
   108841 tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat gatgtagccg
   108901 aagcgcttta tcaggaacta aaacagaaaa acctgattac ccatcagttt gcgggtggca
   108961 ccattggtaa caccatgcat aactactcgg tgctcgcgga cgaccgttcg gtgctgctgg
   109021 gcgtcatgtg cagcaatatt gaaattggca gctatgccta tcgttacctg tgtaacacct
   109081 ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt cgttgcttta
   109141 cgctgattgg cgagtccggg gaacgtacct ttgctatcag ccctggccac atgaaccagc
   109201 tgcgggctga aagtattccg gaagatgtga ttgccggagc ctcggctctg gttctcacct
   109261 cttatctggt gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg aaagccattg
   109321 agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggaactaag tttgtcattg
   109381 ccgagaatcc gcagtggtgg cagcaattcc tcaaagacca cgtctctatc cttgcgatga
   109441 acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca tctgacaagg
   109501 cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg tatatggcgg
   109561 gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg ggcgctatag
   109621 cggaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc cagaatccgc
   109681 tgcgtgtata ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc atgaacacta
   109741 atggagcggg ggatggcgca ttggcagcgt tgctgcatga cattaccgcc aacagctacc
   109801 atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact tattcatcgt
   109861 tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac cagcattcac
   109921 ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct tactgggatc
   109981 gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct ggcatttggc
   110041 tctcgatgcc cgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt tcatatttta
   110101 catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt ttccagcagt
   110161 tccagcatag tacgggcaat ttcacgttca cccatcacta cctgattcgc gccacgttcg
   110221 gtgatatacg tcacttcatc gtcataatgg gcgcgggcga taatctcaat atccgggttt
   110281 ttcgcgcggg cagatgccac aatctcgcct gcttcataac cgttgggaat cgtcaggatc
   110341 agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc cgcattgccc
   110401 aatactgcgc ggaccccgcg ctctcgcagc tcatcaacac gggttcgtga cgtctcaatc
   110461 accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc tacacgaccg
   110521 taacccacca gtagcgcatg gttgcaaata tccactggga tctgcttctc ttcttcgatt
   110581 gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt ctccagtagt
   110641 gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac caggttttgt
   110701 ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa cgcgaactca
   110761 ccaatctgcg ccaggctggc ggcgatggtt aatgccgtac gttgggagtg accaaacagt
   110821 cgcaccagga aaaatgcggc taacgactta ccaaacagaa taatcgccag cgtcgccagc
   110881 actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac ggagacaaaa
   110941 aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg gtgactcagt
   111001 tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga gacatcaaac
   111061 agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag ggtaaacagc
   111121 tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg gcgacctacc
   111181 agcatcataa tggcgataaa tgcgatcact ttgccgatgg tgatccccat atcgactgca
   111241 agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg cagcaacacc
   111301 agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat ttgcccacgc
   111361 tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt actggcggtg
   111421 gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac ggcagagagc
   111481 gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat ggcgatggcc
   111541 tttaccgcca tcaaatcctt cagcgaaaaa tgcaaaccga cgccgaacat cagcaaaata
   111601 acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat
   111661 ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg tagtttattg
   111721 gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt gataagcggg
   111781 gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt ggccggggaa
   111841 accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg aataattatt
   111901 gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa acagaaggcg
   111961 gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt ttgcctttgg
   112021 ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa catccccaat
   112081 agtggcagga aagcacagat tttatagact aactcgatgc tggtgtgatc ggcgataagc
   112141 cccagaactg ccgctcccag acctcccatg ccaaaagcaa aaccgaaaaa gagtccagaa
   112201 accataccga tacgtcccgg aagtagctcc tgagcgtaga ccagaatggc agagaatgcc
   112261 gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag gctggcgtag
   112321 ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac atatttccgc
   112381 ccaattttat cccctacagg cccgccgatc accgtacctg ccgcaacggc aaacaggaag
   112441 gcaaacagat gaagctgagc attctggata gataatccga atttttgcat cagataaaag
   112501 gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag gattaacaga
   112561 atgctgaccg ccagtaccac tttgttgcgc ggcagtggat tgataatcgt cgctttgggt
   112621 tttcctttat tcattcggtg ctgtgccgag taccaacggc taatttgcgc caacaccacg
   112681 atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata aggcgcgata
   112741 atcaccgccg ccagcaaggg tcccagggaa ctgccaaagt tgccgccgac ctgaaagata
   112801 gattgcgcca ggccatgccg cccgccggaa gccatacggg ctacgcgaga agattccgga
   112861 tgaaagaccg atgaaccggt accgaccagc gccgccgcca gcagaactgc gccaaaactg
   112921 cccgccagcg caagcagcac cagaccactt aaggtaaagc acatgccaat tggcaacgac
   112981 cacggcatcg gatatttatc ggtccagtag ccgaccactg gttgcagtag cgaagaggcg
   113041 agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa ttctgactga
   113101 agcagcggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag atgtgagagg
   113161 ctgatagcac ctaaaatacc aaacgatgtt cgggccttgg tcgttgacgc agccgcgccc
   113221 gccacaggct ggggttgttc actcattgcc ataggaaagt cactttttca gggttgcgat
   113281 gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg cgtcgtctga
   113341 aggaagtctc aacgccgaat acagaatttc taatctggat gcagatttat cttcaccgga
   113401 cgcagacttg tctatgatgt cgcgtcatac tatttttcaa cacgttgaaa tcaggtcagg
   113461 gagagaagta tgaaattatt gcagcggggc gtggcgttag cgctgttaac cacatttaca
   113521 ctggcgagtg aaactgctct ggcgtatgag caggataaaa cctacaaaat tacagttctg
   113581 cataccaatg atcatcatgg gcatttttgg cgcaatgaat atggcgaata tggtctggcg
   113641 gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg cggtagcgtg
   113701 ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtctgactt acaggatgcc
   113761 gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat cggtaatcat
   113821 gaatttgata atccgctcac cgtattacgc cagcaggaaa agtgggccaa gttcccgttg
   113881 ctttccgcga atatctacca gaaaagtact ggcgagcgcc tgtttaaacc gtgggcgctg
   113941 tttaagcgtc aggatctgaa aattgccgtt attgggctga caaccgatga cacagcaaaa
   114001 attggtaacc cggaatactt cactgatatc gaatttcgta agcccgccga tgaagcgaag
   114061 ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc ggcgacccat
   114121 atggggcatt acgataatgg tgagcacggc tctaacgcac cgggcgatgt ggagatggca
   114181 cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gtcactcgca agatccggtc
   114241 tgcatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc atgcaaacca
   114301 gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata cgtgggacgg
   114361 gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct gattccggtg
   114421 aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct ttacactcct
   114481 gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa caaaggcaaa
   114541 gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga tcgtgacaaa
   114601 gtgcgttttg tacagaccaa tatggggcgg ttgattctgg cagcccaaat ggatcgcact
   114661 ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga agcaggcgat
   114721 atcagctata aaaacgtgct gaaagtgcag ccattcggca atgtggtggt gtatgccgac
   114781 atgaccggta aagaggtgat tgattacctg accgccgtcg cgcagatgaa gccagattca
   114841 ggtgcctacc cgcaatttgc caacgttagc tttgtggcga aagacggcaa actgaacgac
   114901 cttaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgtatggc gacattaaac
   114961 ttcaatgcca ccggcggtga tggatatccg cgccttgata acaaaccggg ctatgtgaat
   115021 accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc gccgctggat
   115081 gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatccgaaag tgccggatgt
   115141 ttgcatccgg cacaatgctt aatcgcggcg ggcgatatca gcaaatttgg catcgaggat
   115201 ctttgccaga tcgcctgccg ccagttcgat atccagtccg cgcttgccgc cggaaacata
   115261 aatagtggca aattcttgtg cgggggcgtc gataatcgtt ggcagacgtt ttttctgccc
   115321 cagtgggcta atccccccaa ccaggtatcc cgtcgaacgc tgcgcgacca tcggatcggc
   115381 catctcaact ttcttggcac ccagcgcttt tgctactttt ttaagatcca gttgacctgc
   115441 gaccggcgta acggccacgg caaggtgttt catatcaccg ttcactgcca ccagcagcgt
   115501 tttgtagacc tgatccggat tcaaacctaa ttttttgacg acttcatcgc caaaattggt
   115561 ttcagccgga tcgtgctcgt aggtatggat ttgaaacgaa atcttgtttt tttcgagtaa
   115621 tttaactgcg ggtgtcatgg taattttcct gccttaaaca aaaaaacgaa gcaagcatac
   115681 gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag agtattggca ggatggtgag
   115741 attgagcgac aatcgagtta caccgtcgct taaagtgacg gcataataat aaaaaaatga
   115801 aattcctctt tgacgggcca atagcgatat tggccatttt tttagcgcaa catttgcggc
   115861 aaattccctt ctccatacag gtgtagtgca ccgaccgcga ccacatatcg ccccggcggc
   115921 atggcgcgta atttatcccg ccaggcgaga tttcgctgat gcatcagcac atcgtacagc
   115981 gactgactga acgtattggg cagcgttatt tcattatttt gcggcggcgc attcagccac
   116041 cagctcatca tttgttgcag caaccgtgcg ttggtatgcc agtgggtcag cgtatcgtcc
   116101 agcagcgcca gtcctttgtc agggagctgg agcaacatgg caatctggtt ttcagcccct
   116161 tccagttcaa tcacgggttt atgttgttgc ttcgccgcct gcaatagctg gtaatcgata
   116221 ccgtattctg cccgcagccc cagtttttgt gcctgcgtcg cctgaagaac catcgcgatt
   116281 tgccacagcg gttgggtaga aaagagtgag ggagaaatgc ccatctcctg gctaatgtgc
   116341 tgcagatttt ggagttgctc ctcgctaatg cgctcttcca gcgcctcgca ggcaggcaaa
   116401 ttagcaaaag gcgtatcgct ggtggaaaca tccgcctcga cgatcagcgc atcggcgttt
   116461 ttgagctttt tgagcaaacg ggtgggcagg ggagccatat cgtggctacc catatgaata
   116521 ctgccaatca gatgaaaatg gcgattgccg gggagggtga tatcgatggc aggccaggtg
   116581 taatgattac cgcgcagcgc ggcccaaagc gtttttaccc ggtacaacag atccatacga
   116641 cctccctttg tgaaatatca tgctagcgcg cggtgaggga tggcgcaaat ggagttgggg
   116701 acttgtttaa tgtgtttgta tgattcagta tgttcttgca tcgctattca cagggaagca
   116761 acagttaaaa accatgaaac aggcaacaag aaaaccgacg acacccggag atattcttct
   116821 ctatgaatat ctggaaccgc tcgatttgaa aatcaatgag ttagcagagt tgctgcatgt
   116881 tcatcgtaat agcgtcagtg cactgatcaa taacaatcgt aaactcacca ctgagatggc
   116941 attccgtctg gcgaaagttt ttgataccac agtcgatttt aggctaaacc tccaggcggc
   117001 agttgatctt tgggaagttg aaaacaacat gcgcacccag gaagaattgg gacggattga
   117061 aacagtggct gaatatttgg cacgccgtga agagcgtgca aaaaaggtcg cgtaaggcca
   117121 aaacatcgct tcatctcgta ggccggataa ggcgtttacg ccgcatccgg catccgagca
   117181 ccattgcctg atgcgacgct tacccgtctt atcaggccta caaacctgtg cctgaaccgt
   117241 aggccggata aggcgtttac gccgcatccg gcagtcatgc gtcgatgcca aatgcgccgc
   117301 cctaaagcag cgcatccgca atgacgtact tattccttcg gtttaaaccg cagcaaccgg
   117361 ttggcgttac tcactacggt aatcgacgag agcgccattg ccgctccggc aactaccggg
   117421 ttaagcagtg ttccagtaaa cggccacaaa ataccggcgg cgaccggaat accgatgctg
   117481 ttgtagataa aggcaccgag caggttctgc ttcatgttgc gcagcgttgc gcgggaaatc
   117541 gcgagcgcat ccgcaacgcc catcaggcta tggcgcatca gggtaatcgc cgcggtttca
   117601 atggcaacat cactgccgcc acccatcgca atgccgacat ccgcctgggc cagcgctggc
   117661 gcgtcgttaa tgccgtcgcc caccattgcc acctgacgtc cttcactttg cagacgtttg
   117721 atcgcttcgg ctttaccgtc cggcagcact ccggcaatca cttcatcaat tccagcttct
   117781 ttggcgatcg cattggcggt ggttgggtta tccccggtca acatcaccag acgatatccc
   117841 gctttatgca ggcgttgcag cgccgccacg ctatcactac gcaacggatc gcgtacagcc
   117901 agcagggcta ctgctttccc gtcaatcgcc agcagcacag gcgttgctcc ttgcgatgcc
   117961 tgagcagtaa tctccgcttc gatagctttg gtaccaacct gttgctcatt taacagcgcc
   118021 tgattgccca gcaataacgc atgaccttca gcttcaccgc tcacgcccag cccgcgcaat
   118081 gtgcggaaac cgttgacctg cggcagctgc atatcacctg ctttatcgag gatcgcccgt
   118141 gccagcggat ggctggaacc ttgctccagt gccgccgcca gacgcaatgc ctgcgcttca
   118201 tcaacatcag caaacgtttt cactgcgaca acctgcggct tcccttcggt cagcgtcccg
   118261 gttttatcga acacgacggt atcgagtgta ctggcgcgtt gcagcgcgtc ggcgtcccgc
   118321 accagcacgc caaactcagc cgcccgcccg acgccggaaa taatcgacat cggcgtcgcc
   118381 agccccagcg cacacggaca ggcgataatc agtaccgtgg tggcaatcac cagggtataa
   118441 acaatttgcg gtgccggacc aaagaagtac cagattgccg cactgaccag cgcaatcacc
   118501 accaccacag gtacaaatac ggctgagatt ttatccgcca gctgaccgat ttctggcttg
   118561 ctgctctggg cctggcgcac catgcgaatg atccgtgaca gcgtggtatg gctgccaacc
   118621 gcactggcac gaaacagcac actgccgtcc tgtaccactg tcccggcatg gacgctttcg
   118681 ccttcgcctt tttgctgtgg gattggttcg cccgtcagca tcgcttcatc cagccatgct
   118741 tcgccctggg taatctcgcc atctaccggc acgcgatcgc cggtcgtcag gcgcagcaac
   118801 atacctggct gcacttccgc cagaggcacg cttttttcac cttcgtcagt aaccaggcgc
   118861 gccgtcggtg gggttaaatc gagtaatttc tccagcgcct tagaagaacg ctgacgtgcg
   118921 cgcgcttcca gcatatggcc gagattaatc agaccgataa tcatcgcact ggcttcatag
   118981 taaagatgcc gcgcttccat cgggaaccac tgcggccaca ggttgacgct catcgaatag
   119041 agccacgcca cacccgttcc cagcgccacc agcgtatcca tcgtcgctgc accgttcagc
   119101 aggcttttcc atgcactgcg gtaaaaatgg ccgccggcga aaaccatcac cgccagggtt
   119161 atcaggccga taaccaacca caggctgcgg ttgtcagcgg tgaccatcat gttatcgcca
   119221 atcatccccc agaccatcac cgggataccc accgccagtg cgacaattgc ctgccagcgg
   119281 aagcgcttca tcgtagcaac ggcggtttcc tgctggcgct cacggcgttt cgcgtcatct
   119341 tcaatcgctt ccgcgccgta gcccgctttt tccaccgcct gcactaaatc ttgtggggag
   119401 gcactgccca tcaccagcgc agtacgctcc gccaggttta cccgtgcctg agtgacgccc
   119461 ggtacgcttt gcagcgcatt ttgtacgcgg gtgacacagc tggcgcagct catgccgctc
   119521 agcagcaact gctggctgtc atcgtcatcg gcggtcgctg ccggaagcgc ctcagaaacc
   119581 gctgtcagtg cttccgacgg gattgatgac tccgccagcg gtttagcctt tgggtggctt
   119641 acagatgcgt cataacccgc ttgtttgatg gtttcgatca gctgttctgc actggcagtc
   119701 ccggtaacgt gcgcttcagt gatagacaca tccgcttgtt ccacatccgg gcgctgttcg
   119761 agactttctt tcacgcgttt aacgcagtga ccgcaggaca ggccgtccag ggtcaggtcg
   119821 ataatttgtg acataaaaca ctcctttaag acagttttga ctggctgtga taaaggttaa
   119881 accttccagc aaggggaagg tcaagaaatt aataaaccag gcgagtaaaa gtccgtaaag
   119941 attaaaaaat tggctcaatt tgcatcagga ttagacattt atctctttgt tttcctgtag
   120001 ttaagttgcg ggtgctaagt taaatcagga tgcctcaaaa tcggcaccgg ggtgaggaat
   120061 tacctcccgc atctataaaa aggagttaac aaaagatgtt agatgcaaac aaattacagc
   120121 aggcagtgga tcaggcttac acccaatttc actcacttaa cggcggtcaa aatgccgact
   120181 acattccctt tctggcgaat gtgcctggtc aactggcggc agtggctatc gtgacctgcg
   120241 atggcaaggt ctatagtgcg ggtgacagtg attaccgctt tgcgctggaa tccatctcga
   120301 aagtctgtac gttagccctt gcgttagagg atgtcggccc gcaggcggta caggacaaaa
   120361 ttggcgctga cccgaccgga ttgcccttta actcagttat cgccttagag ttgcatggcg
   120421 gcaaaccgct ttcgccactg gtaaatgctg gcgccattgc caccaccagc ctgattaacg
   120481 ctgaaaatgt tgaacaacgc tggcagcgaa ttttacatat ccaacagcaa ctggctggtg
   120541 agcaggtagc gctctctgac gaagtcaacc agtcggaaca aacaaccaac ttccataacc
   120601 gggccattgc ctggctgctg tactccgccg gatatctcta ttgtgatgca atggaagcct
   120661 gtgacgtgta cacccgtcag tgctccacgc tcctcaatac tgttgaactg gcaacacttg
   120721 gcgcgacgct ggcggcgggg ggagtgaatc cgctgacgca taaacgcgtt cttcaggccg
   120781 ataacgtgcc gtacattctg gccgaaatga tgatggaagg gctgtatggt cgctccggtg
   120841 actgggcata tcgcgttggt ttaccgggca aaagcggtgt aggtggcggt attctggcgg
   120901 tcgtccctgg tgtgatggga attgccgcgt tctcaccacc gctggacgaa gaaggcaaca
   120961 gtgttcgcgg tcaaaaaatg gtggcatcgg tcgctaagca actcggctat aacgtgttta
   121021 agggctgatc atgatgaaca cggaaggtaa taacggtaac aaacccctcg gtctatggaa
   121081 cgtcgtttcc atcggcattg gggcaatggt gggggcgggg atcttcgcgc tgctggggca
   121141 ggctgcgttg ctgatggaag cctcgacctg ggtcgccttt gcttttggcg gtattgtggc
   121201 gatgttttcc ggttatgcct atgcgcgtct gggggcgagc tatcccagca atggcggcat
   121261 tatcgacttc tttcgtcgcg gattaggcaa cggcgtcttt tcgctggcgc tctcgttact
   121321 gtacctgttg acgctggcgg tgagcatcgc catggtcgcc cgtgcttttg gcgcttatgc
   121381 cgtgcagttt ttgcatgaag gcagccagga ggagcacctt attttgctct acgcgttggg
   121441 gatcattgcg gtgatgacgc ttttcaactc cttaagcaac catgcggtag ggcggctgga
   121501 agtgatcctc gtcggcatta aaatgatgat cctgttattg ctgattattg ccggtgtctg
   121561 gtcgctgcaa ccggcgcata tttccgtctc tgcgcccccc agctccggtg cgttcttctc
   121621 ctgtatcggg ataaccttcc ttgcctatgc gggctttggc atgatggcga acgcggcgga
   121681 taaagtgaaa gatccgcagg tcattatgcc gcgggcgttt ctggtggcga ttggcgttac
   121741 cacgttactt tatatctcgc tggcactggt tttgcttagc gatgtatcgg cattagagtt
   121801 agaaaaatat gccgataccg ccgtagcgca ggctgcttct ccgctgctcg gacatgtggg
   121861 ttatgtgatc gtcgtcatcg gcgctttact ggcgacggct tcagccatta acgcgaacct
   121921 gttcgccgtg tttaacatca tggacaacat gggcagcgaa cgcgaactgc cgaagctaat
   121981 gaataaatcc ctgtggcggc agagtacctg gggcaacatc attgtcgtgg tgttgattat
   122041 gctgatgacg gcggcgctaa atttaggctc actcgccagt gttgccagcg ccaccttttt
   122101 gatttgctac ctggcggtgt ttgtggtggc gatccgcctg cgtcatgata ttcacgcctc
   122161 gttgccgatt cttatcgttg gtacgttggt gatgttgttg gtgatcgttg gctttatcta
   122221 cagtctgtgg tcccagggga gccgtgcgtt gatatggatt attggctcac tcttactcag
   122281 ccttattgtg gcaatggtca tgaagcgcaa taaaaccgta taacatctct ctgtgcgcag
   122341 tacttcctgt attattgtgg tggcggtcga tattcgcact ggcaaaaaaa cgtgcttgaa
   122401 tatctgttga aaccctttaa caaagcacag gaggcgttgc gcgaacgatg aacatcagcg
   122461 atgtagcaaa aattaccggc ctgactagca aagccattcg cttctatgaa gagaaggggc
   122521 tggtgacgcc gccgatgcgc agcgaaaacg gttatcgcac ctacacgcag cagcatctca
   122581 acgaactgac cttactgcgc caggcacggc aggtgggctt taacctggaa gagagcggcg
   122641 agctggtgaa tctgtttaac gacccgcagc ggcacagcgc cgacgtcaaa cggcgcacgc
   122701 tggagaaggt ggcggagatc gaacgacaca ttgaggagct gcaatccatg cgcgaccagc
   122761 tgctggcact ggcgaatgcc tgccctggcg atgacagcgc cgactgcccg attatcgaaa
   122821 atctctccgg ctgctgtcat catcgggcag ggtgattaag acgagacggc tcggatatgt
   122881 agggttatcc cttctatcgc aatgacttca acatgcgtac ctgcaccgag atcctcgctg
   122941 gcgctgacag gccatgaact gtcaccgacg cgcatatgac cgcgcccgtt gaccagcgga
   123001 gattccagca caaaacgtcg gccaatcagc tgctgcccgc gctggtttaa atgactatcg
   123061 ctgtgctttt gttcgcgcac ccgccgcgac aaccatttcc accacagcca ggcggcgagc
   123121 agcgtcagga tggcaaacat taccccttgc cactcccaac ccagcggcac cagccagacc
   123181 accaggccgg taatcaccgc tgccacgccg ctccacaaca aataaccatt tccgcccagc
   123241 atctcggctg ccagcagcaa accgccgaga ctgagccaga aaatatgtgg atgaacgacc
   123301 attaactcca tcatggctga gtccgtttgt tggcgctgtc tttcaccagc tcggcaatcc
   123361 cggcaatcga ccccatcagg ctgctggcct ctaatggcat catcaccact ttgctgttac
   123421 tggacgaacc gatctgctgt aacgcttcgg tgtatttctg cgctacgaag tagttcaccg
   123481 cctgaatatc acctgaggcg atggcttcag acaccatttt ggtggcgcgg gcttctgctt
   123541 cagcggaacg ttcacgcgct tcagcctgta aaaacgccga ctgacgttcg ccttccgctt
   123601 tcaggatttg cgactgtttt tcaccttcgg ctttgaggat ttccgcctga cggatccctt
   123661 ccgcttcaag gatgtaagcg cgtttggtac gttccgcttt catctgcgcg ttcattgaag
   123721 agataagctc tgccggtggg cgcacgtcgc gaatttcaat acgggtgact ttaatccccc
   123781 acgggttggt ggcttcatcg acaatacgca gcaggcgtga gttgatgctg tcgcgctgag
   123841 agagcatttc atcaagttcc attgaaccca gcacggtacg gatgttagtc atggtcaggt
   123901 tgatgatagc cagctccaga ttgctgacct cataagccgc gcgtggcgcg tcaatcacct
   123961 gaataaagca cacggcgtcg atggtaacgt tggcgttatc tttcgagata acttcctggg
   124021 aagggatatc gagcacttgc tccatcatat tgatcttgcg accaatgcga tccataaacg
   124081 gcaccaccag actgagcccc ggctgtaacg ttttggtata gcgaccaaaa cgttctaccg
   124141 tccattgata gccctgcggc acgattttga cgcccgcgcc gacaatgacc agcgcgacaa
   124201 aaatgagaat cgggataaag ataagcatcg gaaaaacctc ctgttgtacc gtccataatc
   124261 agcaaaattg ctgcttgatt aaacaaatta tacctgatta ctgaaagaga gttccccctt
   124321 attcctgcga aggataaact gtttttagta aaaatcagaa aaagggaaca gcgatgcagg
   124381 aaaatagtcc tttgcttcag ctacaaaacg taggatatct ggcgggtgat acgaagattc
   124441 ttaataacat caatttttcg ctgcgtgctg gcgaatttaa gttaattacc ggtccttctg
   124501 gttgtggcaa aagtacgctg ctaaaaatag ttgcttcatt gatcagccca accagcggaa
   124561 cgttactgtt tgaaggggag gatgtcagca cactaaagcc agaaatctac cgccagcaag
   124621 tctcttactg cgcccagaca ccgacgctgt ttggcgatac ggtatacgat aatctgatct
   124681 ttccctggca gatccgtaac cagcagcctg acccggccat ttttctcgat tttctcgaac
   124741 gcttcgcttt gccggacagc attttgacga aggatatcgc cgagctatct ggtggtgaaa
   124801 aacaacgcat ctcattgatt cgtaacctgc aatttatgcc gaaggtttta ttgctggatg
   124861 aaataaccag tgcgctggat gaaagtaata aacataacgt caatgagatg atccatcgtt
   124921 atgtgcgcga gcaaaatatt gccgtgctgt gggtgacaca cgataaagac gaaattaatc
   124981 atgcggataa agtgattaca ctgcaaccgc atgccggaga aatgcaggaa gcacgctatg
   125041 aactcgcata atattactaa cgaatcatta gcactggcat taatgctggt ggtggtggca
   125101 atcttaatta gccataaaga aaaactggcg ctggagaaag atattctctg gagcgtcggg
   125161 cgagcgataa ttcagctgat tattgtcggc tatgtgctga agtatatttt cagcgtggat
   125221 gatgccagcc tgacattatt gatggtgtta tttatctgct ttaatgcggc gtggaacgcg
   125281 caaaaacgca gtaaatatat tgctaaagct tttatctcat cgtttgttgc cattacggtc
   125341 ggggcgggaa ttaccctggc ggtgctgatt ctctcagggt cgattgaatt tatcccgatg
   125401 caggtgatcc ctatcgccgg gatgattgcc ggtaacgcca tggtagcggt ggggttgtgt
   125461 tacaacaatt tagggcaacg ggtcattagc gaacagcaac agattcagga gaaactgagt
   125521 cttggtgcga cgccgaagca ggcttcagcg atattgattc gcgacagtat tcgcgcggct
   125581 ttaattccga cggtcgattc agcaaaaacg gttggcttag tgagtttacc aggaatgatg
   125641 tccgggctga tatttgccgg gattgatccg gtgaaggcga ttaaatatca gattatggtg
   125701 acctttatgc tgctctcaac cgccagcttg tcgaccatta ttgcctgcta tttaacctat
   125761 cgtaagtttt ataattcgcg ccaccagttg gtggtgacgc aattgaagaa gaaataatgc
   125821 gatgccggat gcgcagcatc gcatccggcg ttgtggttca tgtgccgatc aacggaatat
   125881 caatacaaca atgcatacag ctggcggcga tacttcgacg ccagtgcatc acccgtaccc
   125941 agcgcagcga ggatctcctg gaacgtttta cgcgtctgac cgtctgcggc ggtgagatct
   126001 ttacgcagat gcccgaacag caactccagc gcctcttcat tgcgcccaac ctggtgcagt
   126061 tgcagagcca gttgcgtcgc cagtgcggca tcttctggat tctccgccac ctgctgttgc
   126121 aactgttgaa tttccggcgt atcagccgcc tgcttcagca gttcgatttg cgccaccagc
   126181 ccctggtagc gggtgtcctg atcctgcaac ggaatggttt tcagcaccgc ttccgcatct
   126241 tcagaacggt tcagcgcaat cagcgtttct gccagcagca ggccgatctc cccgttctga
   126301 ttcgacaact gccaggcgtc tttcagcaat ggcagggcat cggtgtaatt gccttcctgc
   126361 atcagttgca tcgcctgctg cgctttcagc tcttcttcgc gcggcagcac tttatccagc
   126421 agggcgcgga tcgcctcttc cggttgcggc ccctggaagc catctaccgg ttgcccgttc
   126481 tggaacagat acacggtcgg aatcgcgcgc agaccaaact gggcggcaat catctgctcc
   126541 gcgtcgcagt ccagcttcgc cagaataaat tgcccgttgt actgcgccgc gaggctttcc
   126601 aggatcgggg ttaactgcaa acagtgctgg ctacgttcag accaaaaata gaacagcacc
   126661 ggagtggtca tcgactgttc aagaacctgt tgcaggttag attcgttaat gttgacaata
   126721 ttttctacgg acatggagtc gctctctgtt gtcgatattt tctttgacat gggggcttaa
   126781 gcgcgcgctt caactcaccc ctgcaatatt ttgtccatca cgcgccccgg cagcaggcgc
   126841 ttaagcacca ttactgccca ggtcaccagc gttaccggat agcgcatctt cggcttctcg
   126901 ctaataaaag catggcgtac tttgtccacc accgcttccg gtcccaacgt aaagcgggcg
   126961 gcgatgccgg gattttcgac tggtttatca ctttgcgtct ggttgacgtt gtcggtgaag
   127021 cgagtacgaa tgggaccggg ttcgatcagg ctgactttaa ttccgctgtg gcgtagctcc
   127081 atgcgcagcg cgtctgacca cgcctccagc gcatatttac tggccgcgta agcgccacga
   127141 cccggcgtgg agattaatcc catcaccgat gatgtcatca caatacgccc ttcaccgtgc
   127201 ggtaacatcg cgggtaacag gcgcatggtg agctggtgtg cgccgaaaaa gttggcggaa
   127261 aactgctgtt ccatctgcgc acggctgatg gtggaaaggg ggccatacat gccgaatccg
   127321 gcattgttaa agatcccata cagacaatta tcggtcaggg cgatcacttc gtcggctgcg
   127381 cgatcaacac tttctggcga atccagatcg atcaacacgc cggtaaatcc catgctgttc
   127441 atacgctcaa catcatccgg tttccggcaa cctgccagca catgaaaacc ctggcgtttt
   127501 aattcgagcg cgctttccag gccaattcca ctggaacatc cggtaattaa gaccgatttt
   127561 tgcataactt tacctgtcag gatctccgtt gctttatgag tcatgattta ctaaaggctg
   127621 caactgcttc gccatccagt cggcaataaa cggctgggcg tcgcggttgg gatgaatacc
   127681 gtcatcctgc atccattgtg gcttgaggta gacctcttcc ataaaaaagg gcagcagcgg
   127741 aacatcaaac tctttggcga gtttggggta aatggcgcta aaggcttcat tataacggcg
   127801 accatagttt gcaggcagac gtatttgcat taacaatggt tcagcgttgg cggctttgac
   127861 atcctgcaaa atctggcgca gcgtttgctc ggtttgctgt ggctgaaaac cacgcaaacc
   127921 gtcattgccg cccagttcaa ccagcaccca acgcggctga tgctgtttca gcagagccgg
   127981 aaggcgcgcc agtccttgtt gcgaggtgtc gccgctgatg ctggcattaa ctaccgacgt
   128041 tttactctgc cacttatcat tcaacaaggc aggccaggcc gcgctggcag acattcgata
   128101 cccggcgctc aggctatcac ccagaatcaa taacgtgtcc gctgcggcgg cacggaaggt
   128161 taacaggacc aggaacagga agggcaaatg ccagcggaaa acattgttga agttcatcat
   128221 cttaagaagt ccgtcggtca gggggagcat gaactctcca tcctcaccgg agttgagctg
   128281 gttgtcaaac gtggcgagac catcgcactg gtgggcgagt cgggatcggg taagtcaacc
   128341 ttgctggcga tcctcgccgg gcttgatgac ggcagcagtg gcgaagtgag tctggtggga
   128401 caaccgctac ataatatgga cgaagaagcg cgggcaaagt tgcgcgcgaa gcacgtcggc
   128461 tttgtttttc agtcatttat gttaattcct acccttaacg cgctggaaaa cgtcgagctt
   128521 ccggctctgc tgcgcggtga gagtagcgcg gaaagtcgta acggggcgaa agcgttgctc
   128581 gaacagttag ggctgggtaa acgtctggat catcttccgg cacagctttc cggcggtgaa
   128641 cagcaacgag tggcgctggc acgagccttt aatggtcgac ctgatgtgct gtttgccgac
   128701 gaacccaccg gcaaccttga ccgccagacg ggcgataaaa ttgccgacct gctgttttcc
   128761 ctcaaccgtg aacatggcac cacgttgatt atggtgaccc acgacctgca actggcggca
   128821 cgctgcgacc gctgcttacg gctggtgaac gggcagttgc aggaggaagc atgattgcac
   128881 gttggttctg gcgcgaatgg cgttcgccgt cgctattaat tgtctggctg gcgctaagcc
   128941 tggcggtggc ctgcgtgctg gcgctgggca atatcagcga tcgcatggag aagggcttaa
   129001 gccagcaaag ccgtgagttt atggcgggcg atcgggcgtt gcgcagttca cgcgaagtgc
   129061 cgcaagcgtg gctggaggaa gcgcaaaagc gcggcctgaa agtcggcaag cagctgactt
   129121 tcgccacaat gacctttgca ggcgacacac cgcagctggc gaacgtcaaa gcggtggatg
   129181 atatctaccc gatgtatggc gatctgcaaa ctaatccccc tggcctgaaa ccgcaggcgg
   129241 gcagcgtatt gctggcccca cgcctgatgg cactgcttaa cctgaaaacg ggcgacacca
   129301 ttgacgtggg cgatgccacc ttgcggattg ccggagaagt gattcaggaa ccggattccg
   129361 gttttaaccc cttccagatg gctccgcgtc tgatgatgaa tctggcggat gtcgataaaa
   129421 ccggagccgt gcagccgggg agtcgggtca cctggcgtta taaattcggc ggcaacgaga
   129481 accagctcga cggctatgag aaatggttgt tacctcagct taaacccgaa caacgctggt
   129541 acggtctgga acaggacgaa ggcgcgctgg ggcgatcgat ggaacgctcg caacagttcc
   129601 tgctgctttc ggcgcttctg accttgctgc tggcagtggc agcggtggcg gtagcgatga
   129661 atcattactg tcgcagtcgc tacgatctgg tggcgatcct caaaacgctg ggggcagggc
   129721 gagcgcaact gcgtaagcta atcgtcggtc agtggttgat ggtgctgacg ctttcagccg
   129781 ttaccggtgg ggccataggt ctgttgttcg aaaacgtgtt gatggtgctg ctcaagcccg
   129841 ttctgcctgc tgcactaccg ccagccagcc tctggccgtg gctgtgggcg cttggcacca
   129901 tgacggtcat ctcgctgctg gtggggctac gaccataccg cttgttgctg gcaacgcagc
   129961 ctttacgcgt attacgtaat gatgtggtag cgaacgtctg gccgctgaag ttttatctgc
   130021 cgattgtcag tgtggtggtt gtgctgctgc tcgccggatt aatgggtggc agcatgctgc
   130081 tttgggcggt gctggcgggc gcggtagtac tggctttgct gtgcggtgtg ctgggctgga
   130141 tgctgctgaa tgtacttcgc cgcatgacgc tgaaatcgct gcctctgcgc ctggcggtta
   130201 gccgcctgtt acgtcagccg tggtcaacgt taagtcagct ttcggcattt tcgctctcct
   130261 ttatgctgct ggcgctgttg ctggtattgc gtggcgatct gctcgaccgc tggcagcagc
   130321 agctaccgcc agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac
   130381 cgctaaaagc gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc
   130441 gggcgcggct gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta
   130501 atcgcgaact caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg
   130561 gtaactggcc gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa
   130621 acgttgccct cggtgatacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag
   130681 tgaccagcct gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc
   130741 cggaaggggc attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg
   130801 gcaacggcat gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg
   130861 gcgcgatttt aaaacaggtc ggtcaggtgc tggagcaggt aagtcgggcg ctggaggtga
   130921 tggtggtgct ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa
   130981 tgcgccagcg tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc
   131041 tgcgcacgac attgtggtgt gagttcgcca tgcttgggtt tttttccggc ctggtggccg
   131101 caattggtgc ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat tttccgtggg
   131161 agccagactg gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt
   131221 tcggcggctg gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg
   131281 ggtgatgaca ggcgttagat tgcctgatgc gctacgctta tcaggcctac gaagagcgtg
   131341 caatatattg atttttcaag attgtgtagg ccggataagg cgtttacgcc gcatctggca
   131401 tgaacagtgt gcattttggc aataaatatg aaaccaactg tcatgtcggt ttttttaaag
   131461 gccgaaggtg gtaaaccttc ggccttagct acgtttcctc agttattcaa taaactgacg
   131521 gatcagttcc agcagcgcga ttagcgcctt aagaacgatg agaactttat cgagaaaagt
   131581 tttcatccgt tgctccctta agggagaaag acaatcacag cttaccctta cgttgcctgt
   131641 aactatgatt ttcttcatgt gctagatgag gactgcctct ctccaaatta gagcaccaga
   131701 atcagcacca cctgtatcct gaccaggact taaaagtact tttccgcact gcggtaatcc
   131761 cttgaggcag tatcactact agcgttggga ttgtatcgcc aacatagctg tttgaaatgt
   131821 aggcatcctg acttataatt cacttgctag atgaggactg tttcatttct gcccggaacc
   131881 accaagtctt gggcggaaaa aataaaaaaa ccgactgtca tgtcggtttt tttatatctg
   131941 tattctggtg caaccattct gttctcacga caatcgccgt ttcagaatac cgcccatcac
   132001 tgcgccttaa cccattccgc cacttccgcc cactcaccgc gaaagacaac tttttccgct
   132061 tttttctcaa gctgatagcg atacatcggg tcgtaatatt cttcaagtaa cggcaccagc
   132121 caggccagat gaccgtcggt gctgccggtg gtgagttgcg ttgtcagtgc tgcatccagc
   132181 cttgcagcca gttcgttata tcgctgtagc cccagccgac gcttaatcgc cgaaagtccg
   132241 tgatgcaggt attcgcaata ttcctgccag ccctgttcgt cgccataagc gtgggtaaaa
   132301 tcatgatgca tacgcaagaa atactcttcg ttcaggcgtt caagacggat ctcaaacgga
   132361 tcttctacca ccgcaatcgc cgcctgagtc attcgctcgc gcagacattc cggcaggtga
   132421 ttcgaaccga tcatccggct ttcgtcttcc agcacccaca ggcgcaaatt ctgacgggcg
   132481 tcggttttta gcatttcggc agccagcagg ttttcaaaac tcgcctggct aagttgtggt
   132541 tgtaaggtgc gaccaaacgc cgaaccgcga tgacgcgcca acccttccag atcaacaccg
   132601 ttcggctgtt gctgcactaa cagcgttttg ccgcagccgg tacaaccgcc aatcagcact
   132661 atcggttttt gtgacagttc aatggtcgcc tgaatcgcag tctggcgcag taccttataa
   132721 ccgccttcca ccagcggata attaatcccc acttcatgca accagcgttg cacaatatgt
   132781 gaacgttgac cgccacgggc gcagcagaga ataccttgcg gattttgcag gcacgctgcc
   132841 cgccaggcgt ccatgcgctg ctgacgaatt tcacccgcca ccagtttatg tcccagcgcc
   132901 agcgctgcgt ctgagccttg ctgtttatag caggtgccaa cggcggcgcg ttcatcgtta
   132961 ttcattaacg gcagattgat agcggcgggc attgcgccgt gctcaaactc gataggggcg
   133021 cgaacatcaa taatgggcgt atcagcaatc agcagggcac gatagtcctg ttccgtgtgt
   133081 ctctcttgca tagttaaaag tgaacctcaa atcagcttgc gcgctatttt acgcgccaac
   133141 gcgcaaggaa acttgatttt taactgcgtg ggttgccgaa aatttctaaa aatccgctga
   133201 tttccggcct gcgttgggta aacaaggtca caatatcttc taccgctttg ccgctgccaa
   133261 atttgcgcca tgccagactc aatggcgaag gagggcgcat cgttgggatt acccggctga
   133321 ccagttgttg attatcgatc attgactggc aaagtgattt tggcaaaaaa ccaatgccaa
   133381 cgcccgccag atgggcggcg attttcgttt ccatatcagg aacaataatc tctttttgcc
   133441 ctggcaatcg ccaggcgacg cgtttggtta aggtgcgggc gctgtcttca atattgaccg
   133501 ccggaaagcg ccgcaactgc gcttctgtta gcggctcttc aacgttcgcc agcggatgat
   133561 ccgccgccat gacaaagcgc cattgcaccg atcctaaggg atcaagacta aaggtatttg
   133621 ccagcgcctc ggttcccgtg acgccgatag ccagtgaaaa accttcgtac aatagcgagt
   133681 cccagacgcc catatagatt tgtcgggaga tgtgaaactg ggtaaagggg taacgttcat
   133741 tcagccacgc cagcaactgg gcgacggcct gggggttgta gagcaggttg ttgatgacaa
   133801 tattcacctg gcgttccacg ccatcattca cctgttgcag ctcgcttggc atactttcca
   133861 gccagctcag ccagtctctg gcctgggaaa gtagatgctc gccagccgct gtcaacgtca
   133921 cgctgcgagt cgtgcggaaa aacagcgcta ctccggtatt ctcttccaga agtttaatgc
   133981 gataactgat cgtcgctgtg gttttacata atcgttctgc cgcttttgaa aaacttcctg
   134041 tttcagcaac cgcaatgaaa gtccgcaagg tttctggatc gaacatcttc aggtatcccc
   134101 ttttaaatcc gcaagttgcg tgattttctt atcctctgat ttatcagtat ttttacatga
   134161 taaccctgtt caatttgtgg actaaatcta gttttggaaa aatattccaa cttttgtatt
   134221 gaagttgttc tcttaaggtt ttagattgcc tgttattgaa accaagctga ccggtcggcg
   134281 gtggttgaac ggaattatgt tacaaggaca aaaagatgaa acttcaggta ttaccgttaa
   134341 gtcaggaagc ctttagtgct tatggcgacg taatcgaaac gcagcaacgg gattttttcc
   134401 atattaacaa tggcctggtg gagcgttacc acgatttggc gctggttgag attcttgagc
   134461 aagaccgtac gcttatcagc attaaccgcg cgcaaccggc gaatctaccg ctgaccattc
   134521 acgaactcga acgtcatccg ctgggtactc aggcctttat cccgatgaaa ggtgaagtgt
   134581 ttgtggtggt cgtggcgtta cgtgacgaca aaccagacct gtcaaccctg cgggcgttta
   134641 tcaccaacgg tgaacagggg gtgaattacc atcgtaacgt ctggcatcac ccacttttcg
   134701 cctggcagcg cgtcaccgat tttctgacca tcgatcgcgg cggcagtgac aactgtgatg
   134761 ttgaaagtat tcctgaacag gaactctgtt ttgcgtgacg cctgcaaccg acttgcataa
   134821 gataaattaa ttgttcattg tttatgctca cttgtaggtc ggagttaacg taggtatgac
   134881 ggaagttaga cggcgcggca ggccaggaca ggcggagcct gtggcacaga agggcgcaca
   134941 ggcgttagag cggggaattg cgattctgca atatctggaa aaaagtgggg gaagttcgtc
   135001 ggttagcgat atttctctca atctggattt gccgctctcc acgacctttc gcttgctgaa
   135061 ggttttacag gcagcggatt ttgtctatca ggacagtcag ttaggctggt ggcatatagg
   135121 attaggtgtc tttaacgtcg gtgcggcgta catccataac cgcgatgtcc tctccgtcgc
   135181 cgggccgttt atgcgccgcc tgatgttact ttccggcgaa acggtcaatg tcgcgatccg
   135241 taacggcaat gaagcagtat taattggtca gctagagtgt aaatcgatgg tcaggatgtg
   135301 tgcgccactg ggcagtcgtc tgccactgca tgcttccggt gcgggcaaag cgctgcttta
   135361 tccgctggcg gaagaggagt tgatgagcat cattctgcaa accggtttgc agcagtttac
   135421 gccaactacg cttgtggata tgcccacctt actgaaggac ctggagcaag cgcgtgaact
   135481 gggctatacc gtagataaag aagagcatgt tgtaggtctg aattgcatag cttcagcaat
   135541 ttacgatgat gtaggtagtg ttgttgccgc tatctccatc tccgggcctt catcaagact
   135601 gacagaagat cgttttgtca gtcagggtga gctggtcaga gacaccgccc gcgatatcag
   135661 cacggcgttg ggactgaaag cacatccata gtgtctgtcg catcccgctc tgcggagcgg
   135721 gtttttttga caaaatttga aagttggaaa atttttccaa ttaatagagg taggaataaa
   135781 atggcaaaaa tgagagccgt tgacgcggca atgtatgtgc tggagaaaga aggtatcacc
   135841 accgccttcg gcgttccggg agctgcaatc aatccgttct actcagcgat gcgtaagcac
   135901 ggcggtattc gtcacattct ggcgcgtcat gtggaaggtg cttcgcacat ggcggaaggt
   135961 tatacccgcg caacggcagg gaatatcggc gtatgtctgg ggacttccgg tcctgcgggc
   136021 acggacatga tcaccgcgct ctattccgct tctgctgatt ccattcctat tctgtgcatt
   136081 accggccagg caccgcgcgc ccgtctgcat aaagaagatt ttcaggccgt agatattgaa
   136141 gcaattgcta aaccggtcag caaaatggcg gttacagttc gtgaagcggc gctggtgcct
   136201 cgcgtgctgc aacaggcatt tcacctgatg cgttctggtc gtccgggtcc ggtactggtg
   136261 gatttaccgt tcgacgttca ggttgcggaa atcgagtttg atcctgacat gtacgaaccg
   136321 ctgccggtct acaaacctgc tgccagccgt atgcagatcg aaaaagctgt agaaatgtta
   136381 atccaggccg aacgtccggt gattgttgcc gggggcgggg taattaatgc tgacgcagct
   136441 gcactgttac aacagtttgc tgaactgacc agcgttccgg tgatcccaac gctaatgggc
   136501 tggggctgta tcccggacga tcatgaactg atggccggga tggtgggtct gcaaaccgcg
   136561 catcgttacg gtaacgcaac gctgctggcg tctgacatgg tgtttggtat cggtaaccgt
   136621 tttgctaacc gtcataccgg ctcggtagag aaatacaccg aagggcgcaa aatcgttcat
   136681 attgatattg agccgacgca aattggtcgc gtgctgtgtc cggatctcgg tattgtctct
   136741 gatgctaaag cggcgctgac actgctggtt gaagtggcgc aggagatgca aaaagcgggt
   136801 cgtctgccgt gtcgtaaaga atgggtcgcc gactgccagc agcgtaaacg cactttgctg
   136861 cgcaaaaccc acttcgacaa cgtgccggtg aaaccgcagc gcgtgtatga agagatgaac
   136921 aaagcctttg gtcgcgatgt ttgttatgtc accaccattg gtctgtcaca aatcgctgcg
   136981 gcacaaatgc tgcatgtctt taaagaccgc cactggatca actgtggtca ggctggtccg
   137041 ttaggctgga cgattccggc tgcgctaggg gtttgtgccg ctgatccgaa acgcaatgtg
   137101 gtggcgattt ctggcgactt tgacttccag ttcctgattg aagagttagc tgttggcgcg
   137161 cagttcaaca ttccgtacat ccatgtgctg gtcaacaacg cttatctggg gctgattcgc
   137221 cagtcgcagc gcgcgtttga tatggactac tgcgtgcaac tcgctttcga gaatatcaac
   137281 tccagcgaag tgaacggtta cggcgtcgac cacgtaaaag tagcggaagg tttaggttgt
   137341 aaagcgattc gcgtcttcaa accggaagat attgcgccag cctttgaaca ggcgaaagcc
   137401 ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttctcgagcg tgtgaccaat
   137461 atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc cgataacgca
   137521 gcggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata aataatgtta
   137581 cgtttctctg ctaatttatc gatgttattt ggagaatatg attttctcgc ccgttttgag
   137641 aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga ctacgacatt
   137701 gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca caatctaccg
   137761 gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg tgaagaagag
   137821 tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa taaaaaaatt
   137881 aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca cgcaacgctt
   137941 gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt attactgatt
   138001 gaacctatta accattttga tattcctggt ttccatctca ccggaactcg gcaggcgctg
   138061 aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat ttatcacatg
   138121 cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa aattggccac
   138181 ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat taattatgat
   138241 tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg tgaatataaa
   138301 ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtaccgtta aaacgtaacg
   138361 ctgttcaggc aatgcttttt taggccgcta agttggcagg ggatcgtgtt gtctgaattc
   138421 aggaaaagcg aaatttaaaa gaggttaatt atgaaactgg gatttattgg cttaggcatt
   138481 atgggtacac cgatggccat taatctggcg cgtgccggtc atcaactgca tgtcacaacc
   138541 attggtccgg ttgctgatga attactgtca ctgggtgccg tcagtgttga aactgcgcgc
   138601 caggtaacgg aagcatcgga catcattttt attatggtgc cagatacacc acaggttgaa
   138661 gaagttctgt tcggtgaaaa tggttgtacc aaagcctcgc tgaagggcaa aaccattgtt
   138721 gatatgagct ccatttcccc gattgaaact aagcgtttcg ctcgtcaggt gaatgaactg
   138781 ggcggcgatt atctcgatgc gccagtctcc ggcggtgaaa tcggtgcgcg tgaagggacg
   138841 ttgtcgatta tggttggcgg tgatgaagcg gtatttgaac gtgttaaacc gctttttgaa
   138901 ctgctcggta aaaatatcac cctcgtgggc ggtaacggcg acggtcaaac ctgcaaagtg
   138961 gcaaatcaga ttatcgtggc gctcaatatt gaagcggttt ctgaagccct gctatttgct
   139021 tcaaaagccg gtgcggaccc ggtacgtgtg cgccaggcgc tgatgggcgg ctttgcttcc
   139081 tcacgtattc tggaagttca cggcgagcgt atgattaaac gcacctttaa tccgggcttc
   139141 aaaatcgctc tgcaccagaa agatctcaac ctggcactgc aaagtgcgaa agcacttgcg
   139201 ctgaacctgc caaacactgc gacctgccag gagttattta atacctgtgc ggcaaacggt
   139261 ggcagccagt tggatcactc tgcgttagtg caggcgctgg aattaatggc taaccataaa
   139321 ctggcctgat acccgcaata aaaatggccg atatcagaaa atgaatcggc cagcaatatt
   139381 aaaaaagaaa gcagccaaag atgttgcttc agtattaaaa ataatatttt tattttattt
   139441 gttcctcata gctagattaa aacaacgtta ttcgatacgt gaaattaaga gggatttatg
   139501 gaacatcaga gaaaactatt ccagcaacgc ggctatagcg aagatctatt gccgaaaacg
   139561 caaagccagc ggacctggaa aacatttaac tattttacct tatggatggg ttcggttcat
   139621 aacgttccca attatgtgat ggtcggcggc ttttttattc tcggcttgtc tacctttagt
   139681 attatgctgg caattatcct cagcgccttt ttcattgccg cggtaatggt attaaacggc
   139741 gctgcgggca gtaaatacgg cgtaccgttt gccatgatcc tgcgtgcttc ttacggcgta
   139801 cgtggcgcac tgtttcccgg attattaaga ggcggtattg ctgccattat gtggtttggc
   139861 ctgcaatgtt acgcggggtc actggcctgc ttgattctga ttggcaaaat ctggccggga
   139921 tttttaactc tcggtggtga tttcacgctg ttagggcttt ctctaccggg cttaattact
   139981 ttcttactct tctggctggt caacgttggt ataggttttg gcggtggcaa agttttaaat
   140041 aaattcactg ccattcttaa cccgtgcatc tatatcgttt tcggcggtat ggcgatttgg
   140101 gcgatttcgc tggtcgggct cggtccaatc tttgactaca ttccgagcgg tattcagaaa
   140161 gcagaaaaca gtggcttcct gttcctggtg gtgataaacg cggtagttgc ggtctgggcg
   140221 gcaccggcgg tgagcgcatc cgactttacg caaaacgccc actcgtttcg tgagcaagcg
   140281 ctggggcaaa cgctgggttt agttgtggcc tatattctgt ttgcggtcgc gggggtatgt
   140341 attattgccg gagccagtat tcattacggc gctgatacct ggaacgtgct ggatattgtt
   140401 cagcgttggg acagcttgtt cgcctcgttc tttgcggtac tggttattct gatgacgact
   140461 atctccacta acgccaccgg taatattatt cctgccggtt atcagattgc cgctattgca
   140521 ccgacgaaac tgacttataa aaacggcgta ctgattgcca gtattatcag cctgctgatc
   140581 tgcccgtgga aattaatgga aaatcaggac agtatttatc tgttcctcga tattatcggc
   140641 ggaatgcttg gtccggtaat tggtgtcatg atggcgcatt attttgtggt gatgcgtggg
   140701 aaaattaatc ttgatgaact gtataccgca cctggcgatt ataaatatta cgataacggt
   140761 tttaacctca ctgcgttttc agtaactctg gtggccgtta ttttatctct tggcggtaag
   140821 tttattccct ttatggaacc gttatcgcgt gtttcatggt ttgtcggcgt catcgtcgcc
   140881 tttgcggcct acgccttatt aaagaaacgt acaacagcag aaaaaacagg agagcaaaaa
   140941 accataggtt aattaatccc gatattgaac attgagttaa aaaccaatct gtattttaca
   141001 aggagtttgt tatgtctttt gatttaatca ttaaaaacgg caccgttatt ttagaaaacg
   141061 aagctcgcgt tgtagatatc gccgttaaag gcggaaaaat tgctgctatc ggtcaggatc
   141121 tgggcgatgc aaaagaagtt atggatgcgt ctggtctggt ggtttcgccg ggcatggttg
   141181 atgcgcacac ccatatttct gaaccgggtc gtagccactg ggaaggttat gaaaccggta
   141241 ctcgcgcagc ggcaaaaggt ggtatcacca ccatgatcga aatgccgctc aaccagctgc
   141301 ctgcaacggt tgaccgtgct tcaattgaac tgaagttcga tgccgctaaa ggcaagctga
   141361 ctatcgatgc ggcacaactc ggtggcctgg tgtcttacaa cattgatcgt ctgcatgagt
   141421 tggatgaagt gggcgttgtc ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta
   141481 tcgacaacga cttccgtgat gtaaacgact ggcagttctt caaaggtgcg cagaagctgg
   141541 gcgaactggg gcagccggtg ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg
   141601 gcgaagaagc gaaacgtgaa ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg
   141661 tatttaccga agtggaagcg attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc
   141721 gtctgcacgt ttgccatatc agcagcccgg aaggtgttga agaagtgact cgtgcacgtc
   141781 aggaaggtca ggatgttacc tgtgaatcct gcccgcatta ctttgtactg gataccgatc
   141841 agttcgaaga aatcggcact ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc
   141901 agaaaggcat gtgggaaaaa ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact
   141961 caccatgccc tccggaaatg aaagccggca acatcatgga agcatggggc ggtattgccg
   142021 gtctgcaaaa ctgtatggac gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc
   142081 tgccaatgtt cggcaaatta atggcgacta acgcagcaga tattttcggt ctgcagcaaa
   142141 aaggccgtat cgccccagga aaagatgccg acttcgtctt cattcagccg aatagcagct
   142201 atgttcttac caatgacgat ctggaatatc gccacaaagt cagcccgtat gttggccgta
   142261 ccattggcgc gcgtatcacg aaaaccatct tacgtggtga tgtgatttac gacattgaac
   142321 agggcttccc tgttgcgccg aaaggtcaat ttatccttaa acatcagcag taatctggcc
   142381 cctgcaatgc ccgtccttgc ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt
   142441 tgcagtcagc cgcgaaagcc tgttatcagg atttcagtgg tttttcttta ttttttgcaa
   142501 cacggttgtg gttcctccta cgctactttc tgcttttcag ttgccgcaaa gtagcctgct
   142561 tacgctcacg caatatgctt ttcttgctac cgcactggcc tgcttcgctc aggcgttctg
   142621 cggtcatcgt cgcgctatta tggaagggcc gggtggcctg tggtggggaa ccatccttac
   142681 tatcaccctt ggcgaagcat cgcgcgggac accgatcaac gatatcgcta ccagcctggc
   142741 ggtggggatt gcgctctccg gcgtgctgac gatgttgatt ggttttagcg gattaggcca
   142801 tcgcctggca cggttattta cgccgtcggt gatggtcttg tttatgttga tgctgggcgc
   142861 gcagctgacc actatctttt tcaaaggtat gctcggactg ccgtttggca tagccgaccc
   142921 aaattttaaa attcagttac caccgttcgc gctctcggtg gcggtgatgt gcctggtact
   142981 ggggatgatt atcttcctgc cgcaacgttt tgcccgttat ggcctgctag tcggcaccat
   143041 aaccggctgg ttgttgtggt atttttgctt tccttcttcg cactcgctct ccggtgagtt
   143101 gcactggcag tggttcccgc tcggcagtgg cggtgctttg tcgccgggga ttattctgac
   143161 ggcggtgatt acaggtctgg taaatatcag caatacctac ggtgcgattc ggggcacgga
   143221 tgttttttat ccgcagcagg gcgcggggaa tacgcgttat cgtcgtagct ttgtggcgac
   143281 cggatttatg acactgataa ccgtaccgct ggcagtaatt ccattttcac cgtttgtttc
   143341 atccattggt ttattaaccc agactggcga ttacacgcgg cgttcgttta tttatggcag
   143401 cgttatttgc ctgctggtgg cgctggttcc ggcactcacg cgactgtttt gcagtatccc
   143461 tttacccgtg agtagcgcgg tcatgctggt ttcttatctg cctttactct tttccgcgct
   143521 ggtgtttagc cagcaaataa cgtttaccgc tcgcaatatt tatcgactcg cattgccgtt
   143581 atttgtcggc atatttttaa tggcattacc gcctgtgtat ctgcaagacc ttccattaac
   143641 gcttcgtcct ctgctcagta acggcttatt ggtcgggatt ttactggctg ttcttatgga
   143701 taaccttatt ccgtgggaac gtatcgaata atttgttgaa aaaggattga taatgaagat
   143761 tgtcattgcg ccagactctt ttaaagagag cttaagtgca gaaaaatgtt gtcaggcaat
   143821 taaagccggg ttttcgaccc tctttcccga tgcgaactat atctgtttgc cgatagcgga
   143881 tggcggcgaa ggaacggtgg atgcgatggt cgccgcgacg ggcggcaaca tcgtgacgct
   143941 tgaagtctgc gggccgatgg gcgaaaaagt gaatgctttt tatggcctta ccggcgacgg
   144001 gaaaacggcg gtgattgaga tggcggcagc aagtggcctg atgctggtcg cgcctgaaaa
   144061 gcgtaatccg ttgctggcct ccagttttgg tacgggggag ttaattcgtc atgcgctgga
   144121 taacggcatt cgccatatta ttctcggcat tggcggcagt gcgacggtcg acggcggtat
   144181 gggcatggcg caagcgcttg gtgtgcgttt tctcgatgcc gacggtcagg tgctggcagc
   144241 taacggcggc aatttagcgc gcgtggcaag cattgagatg gaggaatgcg atccgcgtct
   144301 ggcgaacagc catattgaag tagcatgtga cgttgataac ccgctggtag gggcacgcgg
   144361 cgcggcggcg gtgtttggcc cgcaaaaagg ggcaacgccg gagatggtcg aagagctgga
   144421 acaggggctg caaaattacg cccgtgtttt acaacagcaa actgaaatta atgtctgtca
   144481 gatggcgggc ggcggcgctg cgggcggtat gggtattgcg gcggcggtat ttctcaatgc
   144541 ggatattaaa ccgggcattg aaattgtgtt gaatgcagtc aatcttgcgc aggcagtgca
   144601 gggcgcagca ctggtgatca ccggggaagg gcgcatcgac tcgcaaacgg caggcggtaa
   144661 agcgccgctg ggtgtggcgt cggtggcgaa acagtttaat gtaccggtga ttggaattgc
   144721 tggcgtattg ggtgatggcg tggaagtggt acaccagtac ggcattgacg cggtattcag
   144781 cattttgcca cgtctggcac ctttagccga agtgctcgcc agcggtgaaa ccaatctctt
   144841 caacagcgcg cgaaatattg cctgcgccat taaaataggt cagggaatta aaaactgact
   144901 cttgccttta aagcggatgc tatttatttc gtataagagc gcagtactca tgccggatgc
   144961 ggcatgagta ctatatcctt cctgaaaatc gcgcaaattc tatatattgc agagatcatg
   145021 taggcctgat aagcgaagcg catcaggcaa tgttacaaaa aaagccacgg tataaaccgt
   145081 ggcaaaatcc aacatagcta aaaataatca ggcgagtggt atgacttaaa tctctacgtc
   145141 gcggttgcaa tctttcgagt aaatatagct gaacgcttca ccacgcccta cgccataacc
   145201 agcctgtaaa gaataagcgc ccataaagat gtaatcgcct tttttcaccg ggatccagtt
   145261 attgtcaagg ttataaaccc cctgaccgga aagaatatag gcaccatgtt cctgaacgtg
   145321 tgtttcgata taaccgtggc tggcacctgg cgcaaaagag aggatatgca tgttcatatc
   145381 aaaacctaac tctttgggca ggaaatccag caggatcaca tcatccatac cttcatagtg
   145441 aatgcgttcc agttcgctgg cattaccaga aaccagccac ggtgcatggc cttctaccgg
   145501 aacatagcgg cgcttatata aaaagatttg gctgtcttcg gcctgggcgt taacaaacgt
   145561 cattaaggag cctggtgggc aataaagata gccaccttcg cttaaggcaa atgttttgcc
   145621 ttcggctttg gcagtgatat ttccagagat cacatacagg aacgtttcaa tgccttcgcc
   145681 accgaagccc tgttggttgc caccgttttg atgcagtgtg accagataat caacaaaaga
   145741 ggcacccagc tttggtgtgg agaggattgt cgcgtcacaa ttttcaaagc ccggaataat
   145801 attttttacc agaccgtctg gtgttaacag tgcgaaatta ccgtgtttca caatcgcacg
   145861 gttagccagt aaatcatcgc ggtaaccggt gacgttattt aaatatccca tttataactc
   145921 cttatttctg ccaggcaagt tgataaagca tgagtgccaa cgttttgacc ccttcggcaa
   145981 ggtcggtaat attggtgcgt tccgccgggt tatggctgat cccgttgatg ctggggataa
   146041 aaatcatgca ggttggtacg cgaggcgcga aaatttgcgc gtcgtgcccg gcaccactgt
   146101 gcatcacccg gtaattcagt ttttcgcttt cacacaattc tgtcagggtg gcgaccagct
   146161 ccttattcat cggcacgggt tcttcgtcca tccataaatc gatatcaata ccaatgtcca
   146221 tttcatcgca aatcgcccgc atgtcgtttt ctaactgttg ggtgaaatcg cgcagcacgg
   146281 cagcgtcggt atgacgacaa tcaatggtga acgtggtttt acccggcacc acatttaccg
   146341 tattcgggcg cggctctact ttgccaaagg tcagaaccag cggatcgccc atccttttcg
   146401 ctttttcgac cgactgatgg caaatgcgac tgaaagcgta aactgtatca cgacgataac
   146461 ccatcggcgt ggtgcctgca tggtttgatt cgccgttcag cgttaccgta taacgacgct
   146521 gcccgacaat tgcattcacc acgccaattg attgcccatt actttccagc acacagccct
   146581 gttcaatatg cagttcaaca aaggctttaa tatcctgacg cggagttagt ggggcgttcg
   146641 gaagagtaaa tccgcaagcc ttcatcgcat cgacaaaact atttcctttg gcatcacaga
   146701 tattccgcac gtcgtcagg
//
LOCUS       DAPHJE010000002        95765 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPHJE010000002 DAPHJE010000000
VERSION     DAPHJE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.002"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..571
                     /locus_tag="RH222_000138"
     CDS             53..571
                     /locus_tag="RH222_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV3795212.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            587..1126
                     /locus_tag="RH222_000139"
     CDS             587..1126
                     /locus_tag="RH222_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV3795213.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="RH222_000140"
     CDS             complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="RH222_000140"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV3795214.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="RH222_000141"
     CDS             complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="RH222_000141"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV3795215.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4495..5865
                     /gene="xseA"
                     /locus_tag="RH222_000142"
     CDS             4495..5865
                     /gene="xseA"
                     /locus_tag="RH222_000142"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV3795216.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5862..6077)
                     /locus_tag="RH222_000143"
     CDS             complement(5862..6077)
                     /locus_tag="RH222_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV3795217.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6146..7618)
                     /gene="der"
                     /locus_tag="RH222_000144"
     CDS             complement(6146..7618)
                     /gene="der"
                     /locus_tag="RH222_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV3795218.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="RH222_000145"
     CDS             complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="RH222_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV3795219.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="RH222_000146"
     CDS             complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="RH222_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV3795220.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="RH222_000147"
     CDS             complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="RH222_000147"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV3795221.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="RH222_000148"
                     /gene_synonym="gcpE"
     CDS             complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="RH222_000148"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV3795222.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="RH222_000149"
     CDS             complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="RH222_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV3795223.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13391..14545)
                     /gene="trmG/rlmN"
                     /locus_tag="RH222_000150"
     CDS             complement(13391..14545)
                     /gene="trmG/rlmN"
                     /locus_tag="RH222_000150"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV3795224.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="RH222_000151"
     CDS             complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="RH222_000151"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV3795225.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="RH222_000152"
     CDS             complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="RH222_000152"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV3795226.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDHWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="RH222_000153"
     CDS             complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="RH222_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV3795227.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGLTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSLPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22756..23601
                     /gene="sseA"
                     /locus_tag="RH222_000154"
     CDS             22756..23601
                     /gene="sseA"
                     /locus_tag="RH222_000154"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV3795228.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQVIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="RH222_000155"
     CDS             complement(24094..24870)
                     /gene="sseB"
                     /locus_tag="RH222_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV3795229.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="RH222_000156"
     CDS             complement(25012..26295)
                     /gene="pepB"
                     /locus_tag="RH222_000156"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV3795230.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26354..26554)
                     /gene="iscX"
                     /locus_tag="RH222_000157"
     CDS             complement(26354..26554)
                     /gene="iscX"
                     /locus_tag="RH222_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV3795231.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26566..26901)
                     /gene="fdx"
                     /locus_tag="RH222_000158"
     CDS             complement(26566..26901)
                     /gene="fdx"
                     /locus_tag="RH222_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV3795232.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26903..28753)
                     /gene="hscA"
                     /locus_tag="RH222_000159"
     CDS             complement(26903..28753)
                     /gene="hscA"
                     /locus_tag="RH222_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV3795233.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALVARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVMATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEASEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGNDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28770..29285)
                     /gene="hscB"
                     /locus_tag="RH222_000160"
     CDS             complement(28770..29285)
                     /gene="hscB"
                     /locus_tag="RH222_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV3795234.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWAAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29381..29704)
                     /gene="iscA"
                     /locus_tag="RH222_000161"
     CDS             complement(29381..29704)
                     /gene="iscA"
                     /locus_tag="RH222_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV3795235.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29721..30107)
                     /gene="iscU"
                     /locus_tag="RH222_000162"
     CDS             complement(29721..30107)
                     /gene="iscU"
                     /locus_tag="RH222_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV3795236.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30135..31349)
                     /gene="iscS"
                     /locus_tag="RH222_000163"
     CDS             complement(30135..31349)
                     /gene="iscS"
                     /locus_tag="RH222_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV3795237.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31461..31949)
                     /gene="iscR"
                     /locus_tag="RH222_000164"
     CDS             complement(31461..31949)
                     /gene="iscR"
                     /locus_tag="RH222_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV3795238.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32219..32959)
                     /gene="trmJ"
                     /locus_tag="RH222_000165"
     CDS             complement(32219..32959)
                     /gene="trmJ"
                     /locus_tag="RH222_000165"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV3795239.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33078..33881
                     /gene="suhB"
                     /locus_tag="RH222_000166"
     CDS             33078..33881
                     /gene="suhB"
                     /locus_tag="RH222_000166"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV3795240.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34026..34880
                     /locus_tag="RH222_000167"
     CDS             34026..34880
                     /locus_tag="RH222_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3795241.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35071..36351
                     /gene="csiE"
                     /locus_tag="RH222_000168"
     CDS             35071..36351
                     /gene="csiE"
                     /locus_tag="RH222_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV3795242.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36343..37482)
                     /locus_tag="RH222_000169"
     CDS             complement(36343..37482)
                     /locus_tag="RH222_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV3795243.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37642..38532)
                     /gene="hcaR"
                     /locus_tag="RH222_000170"
     CDS             complement(37642..38532)
                     /gene="hcaR"
                     /locus_tag="RH222_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV3795244.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38668..40029
                     /locus_tag="RH222_000171"
     CDS             38668..40029
                     /locus_tag="RH222_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV3795245.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40026..40544
                     /locus_tag="RH222_000172"
     CDS             40026..40544
                     /locus_tag="RH222_000172"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV3795246.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40544..40864
                     /locus_tag="RH222_000173"
     CDS             40544..40864
                     /locus_tag="RH222_000173"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV3795247.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40861..41673
                     /gene="hcaB"
                     /locus_tag="RH222_000174"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40861..41673
                     /gene="hcaB"
                     /locus_tag="RH222_000174"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV3795248.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41683..42885
                     /locus_tag="RH222_000175"
     CDS             41683..42885
                     /locus_tag="RH222_000175"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV3795249.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            42982..43404
                     /locus_tag="RH222_000176"
     CDS             42982..43404
                     /locus_tag="RH222_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV3795250.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43452..44324)
                     /locus_tag="RH222_000177"
     CDS             complement(43452..44324)
                     /locus_tag="RH222_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV3795251.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44336..45430)
                     /locus_tag="RH222_000178"
     CDS             complement(44336..45430)
                     /locus_tag="RH222_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV3795252.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45463..46461)
                     /locus_tag="RH222_000179"
     CDS             complement(45463..46461)
                     /locus_tag="RH222_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3795253.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46486..47997)
                     /locus_tag="RH222_000180"
     CDS             complement(46486..47997)
                     /locus_tag="RH222_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3795254.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48020..49003)
                     /locus_tag="RH222_000181"
     CDS             complement(48020..49003)
                     /locus_tag="RH222_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV3795255.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49100..52381)
                     /locus_tag="RH222_000182"
     CDS             complement(49100..52381)
                     /locus_tag="RH222_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV3795256.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTPEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52499..53692
                     /locus_tag="RH222_000183"
     CDS             52499..53692
                     /locus_tag="RH222_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV3795257.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHNIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWM
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53765..55018)
                     /gene="glyA"
                     /locus_tag="RH222_000184"
     CDS             complement(53765..55018)
                     /gene="glyA"
                     /locus_tag="RH222_000184"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV3795258.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55346..56536
                     /gene="hmpA"
                     /locus_tag="RH222_000185"
     CDS             55346..56536
                     /gene="hmpA"
                     /locus_tag="RH222_000185"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV3795259.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56581..56919)
                     /gene="glnB"
                     /locus_tag="RH222_000186"
     CDS             complement(56581..56919)
                     /gene="glnB"
                     /locus_tag="RH222_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV3795260.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(56980..58314)
                     /gene="glrR"
                     /locus_tag="RH222_000187"
     CDS             complement(56980..58314)
                     /gene="glrR"
                     /locus_tag="RH222_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV3795261.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58304..59017)
                     /gene="qseG"
                     /locus_tag="RH222_000188"
     CDS             complement(58304..59017)
                     /gene="qseG"
                     /locus_tag="RH222_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV3795262.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59182..60609)
                     /gene="qseE"
                     /locus_tag="RH222_000189"
     CDS             complement(59182..60609)
                     /gene="qseE"
                     /locus_tag="RH222_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV3795263.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61167..65054)
                     /gene="purL"
                     /locus_tag="RH222_000190"
                     /gene_synonym="purI"
     CDS             complement(61167..65054)
                     /gene="purL"
                     /locus_tag="RH222_000190"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV3795264.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAIAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65312..66868
                     /gene="mltF"
                     /locus_tag="RH222_000191"
     CDS             65312..66868
                     /gene="mltF"
                     /locus_tag="RH222_000191"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV3795265.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66865..67401)
                     /gene="tadA"
                     /locus_tag="RH222_000192"
     CDS             complement(66865..67401)
                     /gene="tadA"
                     /locus_tag="RH222_000192"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV3795266.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(67426..68061)
                     /gene="yfhb"
                     /locus_tag="RH222_000193"
     CDS             complement(67426..68061)
                     /gene="yfhb"
                     /locus_tag="RH222_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV3795267.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68270..69118
                     /locus_tag="RH222_000194"
     CDS             68270..69118
                     /locus_tag="RH222_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV3795268.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69174..69434
                     /gene="yfhL"
                     /locus_tag="RH222_000195"
     CDS             69174..69434
                     /gene="yfhL"
                     /locus_tag="RH222_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV3795269.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69628..69708)
                     /gene="shoB"
                     /locus_tag="RH222_000196"
     CDS             complement(69628..69708)
                     /gene="shoB"
                     /locus_tag="RH222_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV3795270.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70129..70509)
                     /gene="acpS"
                     /locus_tag="RH222_000197"
     CDS             complement(70129..70509)
                     /gene="acpS"
                     /locus_tag="RH222_000197"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV3795271.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(70509..71240)
                     /gene="pdxJ"
                     /locus_tag="RH222_000198"
     CDS             complement(70509..71240)
                     /gene="pdxJ"
                     /locus_tag="RH222_000198"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV3795272.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71252..71980)
                     /gene="recO"
                     /locus_tag="RH222_000199"
     CDS             complement(71252..71980)
                     /gene="recO"
                     /locus_tag="RH222_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV3795273.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(71992..72897)
                     /gene="era"
                     /locus_tag="RH222_000200"
     CDS             complement(71992..72897)
                     /gene="era"
                     /locus_tag="RH222_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV3795274.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(72894..73574)
                     /gene="rnc"
                     /locus_tag="RH222_000201"
     CDS             complement(72894..73574)
                     /gene="rnc"
                     /locus_tag="RH222_000201"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV3795275.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73846..74820)
                     /gene="lepB"
                     /locus_tag="RH222_000202"
     CDS             complement(73846..74820)
                     /gene="lepB"
                     /locus_tag="RH222_000202"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV3795276.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(74836..76635)
                     /gene="lepA"
                     /locus_tag="RH222_000203"
     CDS             complement(74836..76635)
                     /gene="lepA"
                     /locus_tag="RH222_000203"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV3795277.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76833..77312)
                     /gene="rseC"
                     /locus_tag="RH222_000204"
     CDS             complement(76833..77312)
                     /gene="rseC"
                     /locus_tag="RH222_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV3795278.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77309..78265)
                     /gene="rseB"
                     /locus_tag="RH222_000205"
     CDS             complement(77309..78265)
                     /gene="rseB"
                     /locus_tag="RH222_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV3795279.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78265..78915)
                     /gene="rseA"
                     /locus_tag="RH222_000206"
     CDS             complement(78265..78915)
                     /gene="rseA"
                     /locus_tag="RH222_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV3795280.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(78948..79523)
                     /gene="rpoE"
                     /locus_tag="RH222_000207"
     CDS             complement(78948..79523)
                     /gene="rpoE"
                     /locus_tag="RH222_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV3795281.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79520..79675)
                     /gene="rseD"
                     /locus_tag="RH222_000208"
     CDS             complement(79520..79675)
                     /gene="rseD"
                     /locus_tag="RH222_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV3795282.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            79931..81553
                     /gene="nadB"
                     /locus_tag="RH222_000209"
     CDS             79931..81553
                     /gene="nadB"
                     /locus_tag="RH222_000209"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV3795283.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDNHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81538..82275)
                     /gene="trmN"
                     /locus_tag="RH222_000210"
     CDS             complement(81538..82275)
                     /gene="trmN"
                     /locus_tag="RH222_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV3795284.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82407..83741
                     /gene="srmB"
                     /locus_tag="RH222_000211"
     CDS             82407..83741
                     /gene="srmB"
                     /locus_tag="RH222_000211"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV3795285.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(83950..84831)
                     /locus_tag="RH222_000212"
     CDS             complement(83950..84831)
                     /locus_tag="RH222_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3795286.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            84934..85521
                     /gene="eamB"
                     /locus_tag="RH222_000213"
     CDS             84934..85521
                     /gene="eamB"
                     /locus_tag="RH222_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV3795287.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(85577..85960)
                     /gene="grcA"
                     /locus_tag="RH222_000214"
     CDS             complement(85577..85960)
                     /gene="grcA"
                     /locus_tag="RH222_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV3795288.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86265..86954
                     /gene="ung"
                     /locus_tag="RH222_000215"
     CDS             86265..86954
                     /gene="ung"
                     /locus_tag="RH222_000215"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV3795289.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87002..88039)
                     /locus_tag="RH222_000216"
     CDS             complement(87002..88039)
                     /locus_tag="RH222_000216"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV3795290.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88246..88665
                     /gene="trxC"
                     /locus_tag="RH222_000217"
     CDS             88246..88665
                     /gene="trxC"
                     /locus_tag="RH222_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV3795291.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88734..89432
                     /gene="tapT"
                     /locus_tag="RH222_000218"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88734..89432
                     /gene="tapT"
                     /locus_tag="RH222_000218"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV3795292.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89464..92124
                     /gene="pat"
                     /locus_tag="RH222_000219"
     CDS             89464..92124
                     /gene="pat"
                     /locus_tag="RH222_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV3795293.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92238..93593
                     /gene="pssA"
                     /locus_tag="RH222_000220"
     CDS             92238..93593
                     /gene="pssA"
                     /locus_tag="RH222_000220"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV3795294.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93639..93962
                     /locus_tag="RH222_000221"
     CDS             93639..93962
                     /locus_tag="RH222_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV3795295.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(93959..95257)
                     /gene="kgtP"
                     /locus_tag="RH222_000222"
     CDS             complement(93959..95257)
                     /gene="kgtP"
                     /locus_tag="RH222_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV3795296.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(95583..95698)
                     /gene="rrf"
                     /locus_tag="RH222_000223"
     rRNA            complement(95583..95698)
                     /gene="rrf"
                     /locus_tag="RH222_000223"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        22824 a        26092 c        24072 g        22777 t
ORIGIN      
        1 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
       61 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
      121 gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
      181 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaactc
      241 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt
      301 tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg cagttggcgg
      361 cggagctgta ggcgcggcag cgggttctat ggtgaatgat aaaaccttag tggaaggtgt
      421 ttctttaaca tataaggaag gcaccaaagt gtatacctct acccaggtgg gtaaagagtg
      481 ccagtttacg acaggtttag ccgttgttat taccacaacg tataacgaaa cgcgtattca
      541 gccaaatact aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
      601 tctttgcgcc atcttggcct ccttaagcta tccggctatc gcctcatcat tgcaggatca
      661 actctctgct gtagcagaag ctgaacagca aggtaaaaat gaagagcaaa ggcagcatga
      721 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
      781 ccaggccgcc gctaacaaaa gagcggcaac ggcagcagca aataagaaag ctcgtcagga
      841 taaactggac gccgaagcct ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      901 acgcagctta gagattcaga aacaaaaact ggcgctggcg aaagaagaag cccgcgttaa
      961 gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
     1021 gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag
     1081 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
     1141 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacagaggg ttttgttcat
     1201 tcatagtgca gggattaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1261 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1321 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1381 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1441 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1501 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1561 agcacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1621 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1681 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1741 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
     1801 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1861 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1921 cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa catatccaga
     1981 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     2041 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2101 ccgaggatga ctttatcgtc gccgacctgc tcgcggatgc gagctacagc atcgtcgata
     2161 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2221 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2281 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2341 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2401 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2461 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2521 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2581 tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg ggccgccgga aagaataatg
     2641 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2701 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2761 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2821 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2881 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2941 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     3001 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3061 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3121 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3181 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3241 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3301 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3361 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3421 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3481 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3541 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3601 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3661 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3721 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3781 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3841 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3901 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3961 taacgaccgg atagcccgca aaaccgttac gctcagtcag ttctttcact tcgcgcagcg
     4021 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4081 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4141 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4201 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4261 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4321 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4381 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4441 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4501 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4561 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4621 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4681 ttccgcaaca gcaaccgccg ggtgaccttc cgtccacagc atggacaaca agttttagtt
     4741 cgcgccaata ttacgctcta cgagccgcgc ggtgactacc agataatcgt tgagagtatg
     4801 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4861 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4921 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4981 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcaccg
     5041 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5101 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5161 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5221 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5281 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5341 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     5401 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5461 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5521 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5581 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     5641 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5701 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5761 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagta
     5821 aaaaacatcc agccggtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5881 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
     5941 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     6001 tccccgcagg atgggcaacg cgcatggccg ttatcctgat caagcacatg ctgacattgt
     6061 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     6121 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6181 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6241 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6301 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6361 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     6421 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6481 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6541 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6601 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6661 ccacaatgac aagtgaacgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6721 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcgtct tcaatggcct
     6781 gcaacgtttt gattacggaa aatttctcta cagcatcggt gattttgccg cgtttacgta
     6841 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattggg atgtagatgc
     6901 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6961 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     7021 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7081 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7141 gcagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7201 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7261 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7321 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7381 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7441 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7501 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7561 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7621 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7681 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7741 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7801 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7861 cgacgttaat ccagtgcaga taaccttcac tgtcaccgac caccaggttg ccattataca
     7921 gcaccggaga agtcagcagg cgatgcagca gatcgctttg tgcccacagc gtaacgccgc
     7981 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     8041 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8101 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     8161 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8221 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8281 cgccccccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8341 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt
     8401 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8461 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8521 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc acaccgccag
     8581 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaggctg acagaccaga
     8641 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8701 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8761 cggaagtact ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8821 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8881 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8941 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     9001 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9061 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9121 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9181 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9241 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9301 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9361 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9421 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9481 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9541 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9601 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9661 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9721 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9781 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9841 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9901 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt
     9961 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
    10021 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
    10081 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10141 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10201 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10261 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10321 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10381 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10441 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10501 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10561 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10621 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10681 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10741 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10801 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10861 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcta tgcgggttca atcatacacg
    10921 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10981 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    11041 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    11101 gccggtgacg ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    11161 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    11221 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt tgatacctcg
    11281 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11341 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11401 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    11461 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11521 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11581 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11641 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11701 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11761 gacgccgtat tccgctactt tcagtgcaat gcgatagtcg aagtggatgt cagccaccag
    11821 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11881 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11941 ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    12001 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12061 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12121 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12181 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtatgg tgcctggccg
    12241 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12301 tcagtaacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12361 gccggcgtag tcacgcctgc ctgatcggtt ggcaacggcg cagcgccatc tggcgttgtt
    12421 gttgccgttg gtgccggggt cgcggcggta tcaacatttg cttgcgaagg cgaaacaacc
    12481 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12541 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12601 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12661 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12721 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtca
    12781 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatag gcgcaacttt tgcagcccgt
    12841 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12901 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12961 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    13021 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13081 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13141 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13201 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13261 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13321 attgcaactt aatacttcat tcataaaaga ttacgccacg gtacataaag taaccgtggc
    13381 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13441 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13501 caccacgggt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13561 ggtcgatacg gctgttcgag ctgcgtccat acggcgcgcc cgggaagggg ttccacggga
    13621 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13681 tgccgtcgtt aacgtggtca agcatcacat actcaatagt gacacggccc tgattggcgt
    13741 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13801 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13861 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13921 tgacgcgacg tttagacagg ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13981 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    14041 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccaa
    14101 taatttccga tacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14161 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    14221 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    14281 atgaacgctg ttcttcaacc acttccggtg cgcggatctc cgccacctct ttcagtttgc
    14341 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    14401 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14461 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    14521 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14581 cctggagggt tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14641 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14701 ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg atttcgcgag cggcagattc
    14761 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14821 agtaccagcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14881 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14941 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgtgcct gttcaacggt
    15001 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15061 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    15121 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15181 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    15241 aatgagtttt agcaacaatc aggggcaaaa cagactattg catgacaaat ttcactgtcg
    15301 cgatttgccc cacatcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    15361 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    15421 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    15481 taatcgcgcc atcgcgaacg cctgtcagct gcagagggag cttggcatcg tgaccgtatg
    15541 gcgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    15601 agggttccag cggtagcggc cagacattta tcatttcttg tcgcgcttgc gggcaatcag
    15661 cggcaacacg tttgccattt tcatccagcc agatggggaa acgaatgcca ttaatgcctt
    15721 cctgctccgg cagtaataga gttggcggct gactcccgtc cagcagccag gttgccaggc
    15781 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15841 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15901 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15961 gcgtgccgtc cggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    16021 cccaggcgtc acgatagcca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    16081 gcaaggcact atccggcaag ggtttcgctt catcagccat aatccggcga atgatccacg
    16141 ccgcccccga cgacattaaa ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt
    16201 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    16261 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc accgttgggc aaatataacg
    16321 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    16381 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    16441 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    16501 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    16561 ccgatcctgg cgatcggatc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    16621 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16681 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16741 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16801 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16861 cttcccttga ctcttttacc tgctcacggg accacacacc ttgcacggcc atccgttcga
    16921 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcga agacggctgg
    16981 gcgcttgcgg caaaaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgact
    17041 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    17101 ggttaagata caaggtcaga atttcacgct tagacagatg ccattccagt tgcaacgcgc
    17161 gccagagctg gcgaattttg ccgccaaatg ttttggggtg aggatcaagc agacgagcaa
    17221 cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg
    17281 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag tgatcttcat
    17341 aattgatcag cgcttcaagg taacgtggag aaacatcttc gattgttacc ggataacgcc
    17401 agatgccgtc agcatcggcg aagcgccata gcggcgtacc atcctgcgcc acgaccactc
    17461 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    17521 gaaaaaggag aaatggcgca gctgccagcg ttatccagca gccgcgtttg gttaacaagc
    17581 gaggcattta cggtcggaca atcagcagat cttcagccgc gccggtcgcc cgccattggg
    17641 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17701 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17761 gatcgtcacg gaactcaatg tgcttaatac tcgcctgctg catctggttc agtaagttct
    17821 gcacttcgcc accactttgc tccaggctgg cgctaccgtt cgccagattc tgattttcca
    17881 gttccagacc cgcaggcagc agatccacga ctaacgcatc cggcacgctg ttactggctt
    17941 ttacctgcaa ccagaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    18001 taccatcagt accaagaata tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg
    18061 cggattgcgg ataaccgctg gcatccatac gcaaccataa cggctgatcg ccactgtttc
    18121 tcacctgcaa ggtggcaagt tgatcgctat tcagattgct gtttagcgtt ttctcgcctg
    18181 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    18241 tacgggcagc taggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaacg
    18301 cctgctgcga aagtgtgttc agcaaagtgt actgctcatc gggtagcagt ttattttctt
    18361 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    18421 tccgctcatc actatttctc ggcgttttca gcgccagcac aatcgcttct tcaccacgcg
    18481 ttgcatcacc catggttttc agcgcaacgc caagttgcag cagcggtaaa ccagaagcgg
    18541 catctgcacg atgctcccag atttcacgca gcgcacccag cggagccttt tgttgacggg
    18601 ccaacaccag cgcagcgtaa gactgtacgg cgaatttact ggctttgaga ttatccgcgt
    18661 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18721 taatggcgtc tgtcggcaag ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18781 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18841 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18901 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18961 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    19021 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    19081 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    19141 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    19201 tctgctgatc ggcaacggtt tcatccggta acgctaaccc gctaatggtg gcctgaattt
    19261 ctccatcgcc ataacctggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    19321 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    19381 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    19441 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    19501 ctatcacttt gctttcgttg ctaccgaagt catctgccgt ccaggcttgc gccatgacgc
    19561 gtaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    19621 gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg attgaccggc ggtttaccac
    19681 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19741 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19801 cctgccacgg atctggcgtg acgtagtcag taatattcag cacaccacta tcaacggcag
    19861 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19921 cggttaatgg ttgattcggc cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19981 ggttttcatc gccaagcggc agatgcaaca ccccaactgc gcgttttggc gtcgcggagc
    20041 gagatttatc gccaggacgt actaccagcg tacttaaata cagatcatga cgattccagg
    20101 ttttatcgac cggaatcgtc agatccagcc cttgagccgg aacatcaatc tcttgccacc
    20161 acaacggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcaa
    20221 tatgcaactt aatggtatcg ccagggcgat aactggcttt atccagtttc agcgtgacac
    20281 ggtcgggtcg cacggcaccg ctcccgtcgc tgttgtcctg ccagctatag ccagcccaga
    20341 aacgcacgct actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    20401 ctaccgggaa actgacctta ccggtttcat ccgcctgaag atccagagtt tgttcatttt
    20461 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    20521 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    20581 cttgcgcatc gctataaacg atgtcaaaac cggcgttact gccttcatca acaatgggct
    20641 gtttcaccgt gctgtcagtg cgataatcgt aaaccgattt cgaggcgaac tgcggacgga
    20701 tccccggcaa tgcatcggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20761 cgcccgattc cagcaaacta ccctggaaaa taacctgtaa tggggaatgc gtttccttcc
    20821 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20881 ccagcgtgcg ggaaagattt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20941 acacagcttc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    21001 gtgcaccata caggtagtac cccaccacgg agaatttcac ttcatctttc ggcgttagcg
    21061 gggttttctc accggtcaga ttcagcgcca tgcgctctgg cataaaatct tcgacgtgga
    21121 aatcccacat ccgatactgg ttatcgcccg tgttagcgcg aatatgccac ataccggttg
    21181 ccgcattgct atcgagtggc caggtaaagt ggtagaggcc attctccggc tgactaacga
    21241 cgctcctgag tacctgccca tcgggtttaa tcacgtctaa cttgatgggt tgattgggca
    21301 acgctttacc gtctgcatca cgcagcaaac cattgaggat taccgtttca cctgggcgat
    21361 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    21421 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    21481 tacgcgccaa cagtaatgcc gcgtttttat cattttccag ctgcacatgc ccctgagcgt
    21541 cactggttgc ctgagtcaga gtcagccctt tctcatttaa taaagagact tcaattcctt
    21601 gctgggccgc gccgttttcc agactttggg taaagatatc cagacgattg tgataacggt
    21661 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21721 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21781 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21841 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21901 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21961 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    22021 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    22081 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    22141 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtaa ctctttaaga ttatctgaca
    22201 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    22261 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    22321 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    22381 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    22441 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    22501 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct
    22561 gccacaatgg cccgttagca acgtcgaata attattatgc gtgagaattt acgttatgta
    22621 attcagtatc acgcccatac aatccgaatt ttagtgagaa ttgtggctca gacagccaaa
    22681 tcagcctctt ttgcttgtcg cttttcacta aacatccgac aatttagcct aaccccggca
    22741 aaaatggaga tgcctatgtc cacgacatgg tttgtaggag ccgactggct cgccgaacat
    22801 attgatgacc cggaaattca gattatcgat gcccgcatgg cgtcgcctgg acaggaggat
    22861 cgtaacgttg ctcaggagta tctgaatgga catattcccg gcgcagtgtt ttttgatatc
    22921 gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc
    22981 gccgtggcga tgcgtgaatt aggcgttaac caggataagc acctgattgt ctatgacgaa
    23041 ggtaatcttt tctcagcccc acgagcatgg tggatgctgc gcacctttgg tgtagagaaa
    23101 gtgtcgattc tggggggtgg acttgcaggc tggcagcgtg atgatctgct gttagaagaa
    23161 ggtgcagtag agctgccgga aggagagttt aacgccgcat ttaatcctga agccgtggtg
    23221 aaagtaaccg atgtattatt ggcaagccat gaaaatacgg cgcaagttat tgatgcccgc
    23281 ccggctgcac gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat
    23341 attcccggtg cattgaacgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg
    23401 accgatgaac tggatgcgat attttttggt cgcggcgtca gctacgacaa accaattatc
    23461 gtcagctgcg gctctggtgt aacggcagcc gtggttttgt tggcactcgc gacgctggat
    23521 gtgccaaacg tgaaactgta cgacggcgca tggagtgaat ggggcgcacg ggcagattta
    23581 ccggttgagc cagtgaaata agctttatac aggcaataaa aaaccgccga atttggcggt
    23641 tttttattgc tggtctggtt cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa
    23701 gcgcgtctca gcggagacaa tactcgccag taactctctt tttgtcaagc aaaagagagt
    23761 tattattgtt ctgttagtgt attatccact gcggcccttt ccgccgtctc gcaagcgggc
    23821 gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa gtgcgtctca gcgcttgcta
    23881 tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga
    23941 agatacggtg cttctgtatc gtacttattg tttctggtgc gctgttaacc gaggtaaata
    24001 ataaccggaa tctctccggc gacaatttac tggtgattaa caaccttcag agcagcaggt
    24061 aagcccgaat gccgcccttt ggggcggcat attttagatt atccgattct gtttaaagtc
    24121 acgcaaaaaa ccaccccagc gacgttcata gaatggcgca atatgttcgg taataaagtg
    24181 gctaattcct ttttcccctt ttttcacctg acaaatatcg atgggttcat cgccaggtaa
    24241 tgtatcggtc gctacacttc ccgccacctg aataatttct tcgatatcac catcggcttc
    24301 aatgccaata agtaaattag gctgtgcctc ttcgttctct ttaattgaac aaataaaagc
    24361 acgcttcaca ggcttaatgg ttttaaataa ggtggtgagt gaatcaatca tttgtgctgg
    24421 cggctctgcg acttccgata atatcagcga ttcaccgcct tccaggattt cctggctgct
    24481 cagcggattt ccctcttcac caatcaacaa actgatttca cgcggcataa attctttacc
    24541 ggttggcagt ttggcattaa ggaagagcgt ttcgccaagt gtcatctcaa acaatgtgcg
    24601 aacgggcatt acgacaaatg cctgttcgtc ttcaaccgcc tgttgaagtg cttctaacga
    24661 ggtgaaaaaa ggaatgacgc tggtgccgtc ttctttttcc cagtgctgta aatcaagcgc
    24721 gctatcttca accacagcct cgccctgcgc cgccgtacca ggcacccaga cggtggattc
    24781 cagtagagta cggaaaaagg ccgggcggtg cgccggttcg gttgctgctt tttccagcag
    24841 gtcttcaagt tcgttttttg tttcggacat aagaaccaca attcattcaa cgttcggggc
    24901 gcaaatgcct gatgcgctac gcttatcagg cctacaaggg attcgcaatt tgttgaattt
    24961 gcagaatttg taggccggat aaggcgttta cgccgcatcc ggcatattag tttacgccgt
    25021 taacagatta gctatcgtgc gcacaccaag tcccgtagca cccgcagacc actgttcaac
    25081 cggcgcttta cggtaagtcg ccgagcagtc gatatgcagc cagccttgct gatagttctc
    25141 aacaaagtgc gacaggaagc ccgccgccgt gctcgcgcct gccggatacg ccgcgcttcc
    25201 ggtattgttc agttcggcaa agttagacgg cagctggctg cggtggaatt ccgccagcgg
    25261 cagacgccag aatggttcgt tctcttgtga ggcactcgcc agcaaacgac cggcaagcgc
    25321 atcgtcaaaa ctgaacagcg cgtgataatc attacccagc gcagttttcg ccgccccggt
    25381 gagggtcgcc gcatcaatga tcatttccgg tttctgcgca ctggcatcaa tcagaccatc
    25441 ggcaagcacc agacgccctt cggcatcagt gttcatcact tcaacttttt taccgttgcg
    25501 ataggtgatg atatcgccca gcttgaacgc attgccgcta atcaggttat ccgcacagca
    25561 gaggaacagc ttcacgcgct tgttcagtcc gcgcgtaatg gcaaatgcca gcgccccggt
    25621 aaccgttgcc gcgccgccca tgtccgactt catcgagtcc ataaacgcag tctgtttgat
    25681 gctgtagccg ccggagtcaa aagtgatacc tttacctacc aggcacgcgt acactggcgc
    25741 ttctttatcg ccagttgggt tgtaatccag cgccagcaat accggagaac gttctgaacc
    25801 gcgtccgacg gtgtgcagcc ccatataacc ttgatcacgc agatcttcac ctttggtgat
    25861 ccgataagtc acacgatcgc ccgcgacgtt gctgatcaga tcaacagcac gctgtgccag
    25921 ttgcgatggt cccaattctt ctgccggtgc gttgatggtg tcacgcaccc agtcgatgat
    25981 catcaggcgg ttatccagtt cctggcgctg ggcatcgtcc agatccggcc actccacttt
    26041 acgcgtgcct ttcggggctt tgtaaccttg ccagaatgcc cagcagcgat ccgcatccca
    26101 gccttcaccg cttaactgaa catgcttgat gcccagaccg tcaatcttgc gcgccgcacg
    26161 ctggatcagc cccagatcgt ctgccccgtt caaatgcagg gtaatgccgt cattattaat
    26221 gctgtaagtt gctttttctc cccagcgcgc gtcggcaggt tgggtagaga gggtaatctt
    26281 catcgcttct gtcattttag ttatccttat tcgtttaaca gcaaaacggg ctacccccag
    26341 gcagcccgtt cattcactcg gcttcgtcca gccagactaa caaaatcgct tcgaggattt
    26401 tctcgttgga tgcctgcggg tcgtcgtcga aatcttccag atcgcaaatc cactgatgca
    26461 tatcggtgaa tcgaaccgtt ttcggatcaa gatcgggata cgcatcgtac agtgcttcgc
    26521 caatttcgcg gctatcggtc cacttaagtc ccatactaac ctctgttaat gctcacgcgc
    26581 atggttgata gtgtaacgcg ggatttcgac cactaaatct tcgtcggtaa cgcgcgcctg
    26641 gcagcttaaa cggctttccg gctccagccc ccaggctttg tccagcatgt cgtcttcctg
    26701 ctctgagctt tccggcagtg agtcaaaacc ttcacgaacg atgcagtggc aggtggtgca
    26761 agcacaggat ttttcacagg cgtgttcaat ctcgataccg ttacgcagag ctgcgtcgag
    26821 aatggtttca ccgctattag cttccagaac agcgccatca gggcagagat cctgatgagg
    26881 caaaataaca atctttggca tattaaacct cgtccacgga atggcctttc agcgcacgac
    26941 gaaccgactg gtccatgcgg cgagcggcga aatcctgggt ttgtttgtct acgtttttaa
    27001 tcgcttgttc gatggcgtca acgtcattgc cctgcgccac ttcactcagg tgagcggcag
    27061 catcgtcaat gacctgacgt tctgcggcgc ttaacagcgc ggcatcagca gccagcgcgc
    27121 cgtgcagact ttccagcaca cgcgccgctt ctactttttg ttctgccagc attcgggctt
    27181 ttacgtcctg ctcggcatag ctcattgagt ctttgatcat cgaagcgatt tcgctatcgg
    27241 tcagaccgta agacggtttg acctgaatag acgcctcaac gccggtggat ttctccatcg
    27301 ccgtcacgct caaaagaccg tcggcatcga cctggaacgt cacgcgaata tgcgcaccgc
    27361 cggccggtag cgccggaata ccacgcagcg caaaacgcgc cagtgagcgg cagtcctgca
    27421 ccagttcgcg ctcaccctgc attacatgga tagacatcgc cgtctgacca tctttaaagg
    27481 tggtgaaatc ctgagcgcgg gccaccggaa tagtggtatt acgcggaatc actttctcca
    27541 ccagaccgcc catcgtttcg aggcccagcg acagcgggat cacatccagc aacagcattt
    27601 cgctgtccgg cttgttaccc accagaatat ccgcctgaat cgccgcgcca atagcgacga
    27661 ctttatccgg gtcgatggaa gtcagcggtg gacgaccgaa aaattcgcct acccgttcac
    27721 gcaccagcgg cacgcgagta gaaccgccaa ccatcaccac ttccagcact tcagaagcct
    27781 ctacgccagc gtctttcagc gcgcgacgac aggccagtaa ggttcgtttt accagtggcg
    27841 cgatcagttc attgaattgt tcacggctga tttcgccctg ccagcccgca acattcacgg
    27901 tcacagagtc cgcatcgctc agcgcgattt tggctgcaat ggcggcatcc agcagttcac
    27961 gctgaacgcg gttatcgcta cgatcaggaa tgcccgcctg ctcgcgaatg taatccgcca
    28021 gcagatggtc gaaatcatcg ccgccgagcg cggaatcacc gccggttgcc atcacttcaa
    28081 acacgccgcg acttaagcgc agaatggaaa tatcaaacgt cccgccaccg aggtcataaa
    28141 cggcgatcac gccttcctga ccggaatcca gcccgtaggc gatagccgca gcggtcggtt
    28201 cgttaagtaa gcgcaggacg tgaaggcccg ccagacgcgc cgcgtctttg gtgccctgac
    28261 gctgggcatc gtcaaagtac gccggaacgg tgataactac accatccagc tcgcctgcca
    28321 gggcttcagt tgcccgcgcc accagtgctt tgaggatgtc cgcagaaacg cgcaccgggt
    28381 tcagcagccc cgccgccgtt tcaatcatcg gcaggccgtt ttcgctggcc tggaattgat
    28441 aaggcagatg cggatagcgt tgctggatat cagccagcga gcgccccatc agacgtttaa
    28501 cagaactaat ggtgttggcg gtgtcaagcg ctgcgttagt acgcgcgtca taacccaccg
    28561 aatgcccttg ctgttgatag tgaacaacag atggcagcag gtgacggcct tcatggtcgg
    28621 ctaacgtttc ggcctgaccg ctgcgcactg tcgccaccag cgagttggtt gtgcccaggt
    28681 caataccggc cgccagacga cgctgatgcg gcgcagcact caaaccaggt tcactaattt
    28741 gtaataaggc catgtttagc ttccagaaat taaaaatcga gcagtttttc ttcgagttgt
    28801 tcggcactgc ttcgcagttt atcgagaaaa cgcagcttac gcacggtatc cgccgccgcg
    28861 gcccacgtct cgttgtctaa ctgttcaacc atcaactgat ggcgggtatc aaacatcttt
    28921 ttcacacgtt tgataaagct ttccagccgc gcttcatctt tcgcctgttc gatctcgtcc
    28981 agctcttcgc gcagctccag ctgttccata agaaacgcgg tgtcgcgcac agtatgctgc
    29041 tcgctggcga gatcaaaacc gtgcaaagaa agcaaatatt ccgcgcgcat taacggatga
    29101 cgcagcgttt gccatgcctg gttaatggtt gcagattgct gtacggcggc gagttgttcc
    29161 gcctggcttc cgctggcgaa tttatcagga tgatactgac gttgtagatc ctgaaaacgc
    29221 aggctcagtg cctgggtatc gagttgataa cgggcaggca agccaaagag ggtgaagtaa
    29281 tccataacat tctcagggct gcgatccgcc gcgataacgg cgaatcaggt aaaacaaacc
    29341 ccacgcgcag gcgaccacgg tggggttatc ggtatgcgca tcaaacgtgg aagctttcgc
    29401 cgcaaccaca ctcatcttta acgttcgggt tggtgaattt aaacccttcg ttcaggcctt
    29461 cttttacgaa gtccagctgc gtaccgtcca gaaattgcag gcttttgcca tcgaccacga
    29521 ctttcacgcc tttgtcttca aacacgatgt cttccggcgt cggttcgtca acaaattcca
    29581 gtacataagc catacctgaa catccggagg ttctcacgcc cagacgcagg ccaaaccctt
    29641 tgccgcggtt agccagaaag gtattgactc gcgctgctgc actgtcgctc agtgtaatcg
    29701 acataaccaa acctcaactc ttattttgct tcacgtttgc ttttatagtc cgcaatggcg
    29761 gctttgatcg cgtcttctgc cagaatagaa cagtgaattt tcaccggcgg cagttcaagt
    29821 tcttcagcaa tatcggtgtt tttgatcgcc tgcgcttcgt cgagagactt ccctttcacc
    29881 cattcggtga ccagggagct ggaagcgata gcagaaccgc agccgtaagt tttaaaacgc
    29941 gcgtcttcaa tgataccttc atcgttgact ttaatctgca acttcatcac gtcgccacag
    30001 gccggcgcac ccaccatgcc gctgccgacg ttctcgtcgt tgttgtcgaa ggaacccacg
    30061 ttacgcggat tctcgtaatg atcgataact ttttcgctgt aagccattat aaattctcct
    30121 gattccgata ccgattaatg atgagcccat tcgatgctgt tcagatccac gccctgcttg
    30181 tacatttccc acagcggaga aaggtcacgc aaacgaccga tggatttacg aactaactcg
    30241 atggtgtagt cgatctcttc ttcagtagta aaacgaccta aagagaaacg gatagagcta
    30301 tgtgccagct cgtcgttcag ccccagcgcg cgcagcacgt aggacggttc gaggcttgct
    30361 gacgtacagg cggaacctga agaaaccgcg aggtctttca gcgccataat cagcgactca
    30421 ccttcaacgt agttaaagct gacgttgaga atgttcggcg caccgtgttc gaggtcaccg
    30481 ttcaggtaaa cttcttcgat atctttgatg ccgttccata gacggttacg caggccgcgc
    30541 agacgttcca tctcggtcgc catctcttct tttgcgatgc gataggcttc gcccatgccg
    30601 acgatctggt gaacaggcag agtgccggaa cgcataccgc gttcgtgacc gccgccgtgc
    30661 atttgcgctt cgatgcgtac gcgcggttta cgacgcacat acagcgcacc gatacctttc
    30721 gggccataga ttttgtgacc ggagaaagac atcaggtcaa ctttcaactg gctcaggtcg
    30781 ataggcagtt tacccacgct ctgggttgca tcaacgtgat agataatgcc acgagcacgg
    30841 cacatttcgc cgatagccgc gatatcctgc accacgccga tttcgttatt tacgtgcatg
    30901 atggacacga ggatggtgtc gtcacgcatc gctgcttcaa gttctttcag gtcgataatg
    30961 ccgttacgct gcggtgccag gtaggtgact tcaaaacctt cgcgctccag ctgacggcag
    31021 gtatccagta ccgctttgtg ttcggttttg ctggtgatga tgtgcttgcc ttttttctga
    31081 taaaagttgg ctgcaccttt gatcgccagg ttgtcagatt cggttgcacc agaggtaaag
    31141 acgatttcac gcggatcagc gccgaccaga tcggcaatct gattacgggc gatatctacc
    31201 gcttcttcag cctgccagcc gaaacggtga gaacgggagg ccgggttacc aaaggttccg
    31261 tccatcgtca taaactgcat cattttctcg gcaacacgcg ggtccaccgg cgtggttgcg
    31321 gagtagtcga gataaatcgg taatttcatt gctctataaa ctccgtacat cactcaatgc
    31381 aaggaatcag gctaccggct ggatgtacga ccgtgtttac ggagtattta gcactccggc
    31441 ctgattctga attcttttta ttaagcgcgt aacttaacgt cgatcgcgtc ttgtgtgcgg
    31501 gtgcgtggcg cgtcgtgagt atgctgacga ccagacacat ccagcacttc ctggttatta
    31561 accagttcgc ctaaagtaat gttgttgaga aaaccggtga gacggtcgct caaatcacgc
    31621 cacagcgcgt gggtcaggca tttatcgccg ccctggcagc cgcctttacc ctgacaacgg
    31681 gtggcatcta cagattcgtc aacggcgcta attacttcgc caacggcgat gctgctggca
    31741 tctttgccta acagataacc accgcctggt ccacgtacgc tggaaaccag accattttta
    31801 cgcagacggg aaaacagttg ttccagataa gaaagggaaa ttccctgacg ttcggaaata
    31861 tcagccaacg gtaccgggcc cgcttcagag ttgagcgcaa cgtcaagcat tgcggtcacg
    31921 gcatagcgcc ctttagatgt cagtctcatg tcttacttca cctcaaactc gcccctgccc
    31981 ggggtttttt attgtaaaag tgggggtatt gcatagcagg gtcaagtctg acattcccga
    32041 gtaaattggt caactattta cttgactgat ttagtcgggt atttaacctt cagtgccatt
    32101 tttttatcgt ggcgtttgta cctgttgtcg gatgcggtgt gaacacctta tccgacctac
    32161 ggttctgtac attgtaggcc tgataagacg cgttaagcgt cgcatcaggc aaccactgtt
    32221 attcggcctt gttaccttta ttctgctgct caatagaagc cagaatcccg cgcaggatat
    32281 tcaattcctg gctttccggg cgcgcacggg taaacagacg gcgcaattta ttcatcacct
    32341 gccccggatg gttttcacgg ataaaaccgg ttgccagcag cgtttgttcc agatgaccat
    32401 aaaaacgctc cagatcatcg accagcggat acggcgtctc ttcatgctcg acctgctcgc
    32461 cattttcctg agttgccagc caggccatgc gcacttcata ggcaataacc tgaaccgcca
    32521 tcgccaggtt cagcgagctg tattccgggt tagccgcaat cgcgacatga taatggcatt
    32581 tctgcaactc ttcattggtc aggccgacac gttcgcgacc aaaaaccagc gccaccgggg
    32641 tatttgccgc ttcagcaacg cttttcaaac cgcattcgcg cgggtcgagc atcggccacg
    32701 gcagcgtgcg ggaacgtgcg ctggtgccca ccaccaggct acaaccggct aacgcttcgt
    32761 cgagcgtatc gacgatgtga gcattaccaa tcacatcgct ggcccccgcc gccagggcaa
    32821 tcgcctggga gtcaggtttc accagtggat taaccagcca cagattggtt aatcccattg
    32881 ttttcatggc acgggcaaca gaacccatat tgccggtgtg tgacgtctcc accagcacaa
    32941 ttcgaatatt ttgcagcatt gtctttcttc gtctaaagat tattcgcgca tcttatcata
    33001 aaacgaagac agatgccgat ctcgctgcta tactctgcgc cgttttcccg ttctttaaca
    33061 tccagtgaga gagaccgatg catccgatgc tgaacatcgc cgtgcgcgca gcgcgcaagg
    33121 cgggtaattt aattgccaaa aactatgaaa ccccggacgc tgtagaagcg agccagaaag
    33181 gcagtaacga tttcgtgacc aacgtagata aagctgccga agcggtgatt atcgacacga
    33241 ttcgtaaatc ttacccacag cacaccatca tcaccgaaga aagcggtgaa cttgaaggta
    33301 ctgatcagga tgttcaatgg gttattgatc cactggatgg cactaccaac tttatcaaac
    33361 gtctgccgca cttcgcggta tctatcgccg ttcgtatcaa aggccgcacc gaagttgctg
    33421 tggtatacga tcctatgcgt aacgaactgt tcaccgccac tcgcggtcag ggcgcacagc
    33481 tgaacggcta ccgtctgcgc ggcagcaccg ctcgcgatct cgacggtact attctggcga
    33541 ccggcttccc gttcaaagca aaacagtacg ccactaccta catcaacatc gtcggcaaac
    33601 tgttcaacga atgtgcagac ttccgtcgta ccggttctgc ggcgctggat ctggcttacg
    33661 tcgctgcggg tcgcgttgac ggtttctttg aaatcggtct gcgcccgtgg gacttcgccg
    33721 caggcgagct gctggttcgt gaagcgggcg gcatcgtcag cgacttcacc ggtggtcata
    33781 actacatgct gaccggtaac atcgttgctg gtaacccgcg cgttgttaaa gccatgctgg
    33841 cgaacatgcg tgacgagtta agcgacgctc tgaagcgtta atgactcagg cgggtgatat
    33901 caatcacccg cccccgcctt tcaggcgcta ttccgaaata cttcctcacc gctttacttt
    33961 ctttcgtcac tctcccacca tctttccccg attaatggat gataaagaag tcgcaaggaa
    34021 aggttatggc actgccagtg aacaaacgcg ttcccaaaat tctgtttatt ctctttgttg
    34081 ttgccttctg cgtttattta gtgccgcgcg ttgccatcaa cttcttctat tatcccgacg
    34141 ataaaattta cggtcccgat ccctggtcgg cggaatccgt cgaatttacg gctaaggacg
    34201 gtactcgtct gcaaggctgg tttatccctt cttcgacggg ccctgctgac aacgccatcg
    34261 caaccatcat tcatgctcac ggcaatgccg gaaatatgtc cgcccactgg ccgctggtca
    34321 gttggttacc cgagcgtaat ttcaacgttt ttatgtttga ttatcgcggg tttggtaaat
    34381 caaaaggcac gccgtcccag gccggattgc tggacgatac gcaaagtgcc atcaatgtgg
    34441 tgcgccatcg cagtgatgta aacccacaac gtctggtgct gttcgggcag agtattggcg
    34501 gggcgaatat tctggatgtt attggtcggg gtgatcgtga aggcatacgt gcggtgatcc
    34561 tcgactccac atttgcctct tatgcaacca tcgccaacca gatgatcccc ggcagtggct
    34621 acttacttga tgagagttac agcggcgaaa attatatcgc cagcgtcagc ccgatcccgc
    34681 ttttactcat tcacggtaaa gctgatcacg ttatcccatg gcagcacagc gaaaagttgt
    34741 atagcctggc aaaagagcca aaacggttga tcctaatccc ggatggcgaa cacattgatg
    34801 ctttttccga tcgtcacggc gatgtttatc gcgaacagat ggtggacttt atccttagcg
    34861 cgttgaatcc acagaactaa cccatgatcg ctagcacgat aatcattcac aaaaccacct
    34921 taagacatgc taatccactg gtcagaacag tttaagatga gaaaaattct gtgacgcttg
    34981 ccaacatttc tgatgattag cattcccttc gccatttccc tgagcaaact ttagctattc
    35041 ttatcaatta tgcttatggg agatctacag atgatgccta cgcttgctcc accatctgtc
    35101 ctttcggctc cccagcgccg ctgccagatc ttgctgacgc tctttcagcc ggggttgacc
    35161 gccaccacgg caactttcag cgagcttaat ggtgtggatg atgatattgc cagtcttgat
    35221 atcagcgaaa caggacagga gatcctgcgc tatcatcaac tcacactgac ggctggttat
    35281 gacggtagct accgggttga aggtacagtg cttaaccaac gtttgtgttt atttcactgg
    35341 ctacgacgtg gtttccgtct gtgtccatca tttattacca gccatttcac ccccgccctg
    35401 aagagtgaac tgaagcggcg cggaattgcg cgtaactttt acgacgatac caatctacaa
    35461 gcgttagtga atctctgctc ccgacgactg caaaaacgct ttgaaacgcg cgatattcat
    35521 ttcctgtgtc tgtatctgca atattgtttg ctgcaacacc acgctggaat tacgcctcag
    35581 tttaatccgc tccaacgtcg ctgggcggaa tcctgccttg aatttcaggt agcgcaggaa
    35641 attggacgcc actggcagcg tcgggcgctc cagcctgtac cgcctgatga gccactgttt
    35701 atggcattac ttttttccat gttgcgggtt cccgatccat tgcgtgatgc gcatcagcga
    35761 gacagacaat tgcgtcagtc tatcaaacgt ctggtaaacc attttcgtga gctgggaaat
    35821 gttcgttttt atgatgaaca ggggttatgc gatcagcttt atacccacct cgcccaggcg
    35881 ttaaatcgca gtttgtttgc catcggtatt gataataccc tgccggaaga gttcgccaga
    35941 ctgtacccac gcctggtgcg caccacccgc gcggcgctgg ccggatttga aagtgaatac
    36001 ggcgtccatc tttctgatga ggaaagtggt ctggtcgcgg tgattttcgg tgcctggcta
    36061 atgcaggaaa acgatctgca tgaaaaacag attattctac tgaccgggaa tgatagcgag
    36121 cgagaagcgc agattgagca gcagttacgc gaactaacgt tactgccgct caacattaag
    36181 catatgtcgg taaaggcatt tttgcagaca ggcgctccgc gtggcgcggc actgattatt
    36241 gcgccttata ccatgccatt accgctcttt tcaccaccgc tgatctatac ggacctgacg
    36301 ctgacaacac atcaacagga gcagatccgc aaaatgctcg aatcagcatg agggaacaac
    36361 tttcgggcgc aaaaacattg ccggcagcgc caccagcgcc attacccaga acacgccgtg
    36421 gcccagatat tgatacagga aaccggcgaa aacggtcatg atagcgatac tgccgcccat
    36481 cgcgacggca gagtaaaccg cctgtaaacg gatgacttcg ctaccctggc gagcagcaat
    36541 ataacgcatg gcggccaggt ggcagaccgt gaaggtgccg caatgcagaa tttgcaccac
    36601 tatcaaccac ggcaacgccg tagttgctcc cataatgccc cagcgcacta cgccgcaaat
    36661 cgccgagatc aacagcatat cgcgtgcact acaacggcgg aaaagtttat tactcagcgc
    36721 aaagataatg acttccgcca ccacgcccag cgaccacaaa taccccaccg ccgaggccga
    36781 gtagccagct gcctgccagt aaatggcgct aaaaccgtaa taggccgcat gtgccccctg
    36841 caataaacaa acgcaggcca gaaagcgcca gttctggcga accagcgcca accacgctga
    36901 ccaaccggtg ctctcctgct ggcggcttgc cccttgtggc tgaatcgtcg gacggatgag
    36961 aaagccgagc agcatggatg ccacgcccaa cgtcaacagc gcgaggatca cccgataatc
    37021 aaacatagtg accagtttgc ccgtcagcgc cgagccaatg acaaacgcca ccgagcccca
    37081 cagtcgcact ttgccgtaat caagcgggaa ctgcttttgc cacgtattcg ccagtgcatc
    37141 ggtcaacggt accagcggtg agaaaaagag gttaaagcca atcatcacca gcatcagcca
    37201 cgctacgtgc gcccccgccc agaaggcgac agcaaagaga agtgtcagca gtgccagcac
    37261 gcgcaaggcg gaaatcaggc gggaaggatc gctgacgcgg ggcgcgatga gcaaactccc
    37321 gagaaaacgg gcaaccagac ctgcccccaa taacaggccg atggtttctg gcgttaaacc
    37381 aatcccttta agccagacgc tccagaaagg tagaaaaatg ccgtaactaa aaaagtatgt
    37441 gaaatagccg agcgccaacc agcgcgtgga ttgcaaaacc atgagtccct cccgtcagga
    37501 ggcgttagtc tggggttaat cgaatgttgg tgcaagttga aaaataagaa tattcgagcc
    37561 tgcttcccga tgtctgtaac gccagttcac aagcactaaa aaactgagat ccgcgacgca
    37621 tgttgcaaaa ataatgcgta attatgccgt tacgcttgcc aaacgttcct gcacaatggc
    37681 gatgaaatcg cgtaatgccg gcttcatctc ccctttcttc cacgccatca gtaaagcaat
    37741 ggaaggcaca tttccggcaa tgggacgaaa aacaacctgt ccggtattaa aattattcat
    37801 ataaccgggt atcaaagtga cgcccagccc catgcccacc agattcatgg tcaccagaat
    37861 attcgttgcc acctggacga tatttggctg gctgttttct tgcgcaaacc acgctttaac
    37921 gatcggcgca agcgaaccgg aatacgccgg atcggtactg acgaaattca cgccatccag
    37981 ttgcgcggcg gtgatctctt tttcatgtgc taaagggtga tcaaccggta acacaaccac
    38041 taatggttcg tcaaaaagct ccagataatc aatctccggg ctataaacag gatggcgcat
    38101 taagccgacg tcgagttcac cacggcgaat tttttcctcc tgttgcgtgg tgattaaact
    38161 caccagctca atcaaggtgt ctggctgtct gagacgaaac atcggtaata cttttggcag
    38221 taaattcact tccgccgatg gcacaaagcc aatggttaat tgtctgtctt cctgaacaat
    38281 tttccgcgcc cgtaatttgg cattttccgc ttgttcgagg attgccagcg cgtcctggag
    38341 aaaacattct cccgccgctg tcaacgcgac tttgcgctta tccctcacca gtaacggaac
    38401 accgacacag ttttcaagat cgcggatctg gctgcttaac gaaggctgtg aggtatgcag
    38461 tttttccgcc gcacgggtaa agttcagtgc ctgcgccact gcgacgaaat agcgtaaatg
    38521 ccgtagttcc atcaccttcc ccttgttatc gaaaaaacgt ctcagctggt agaaattaac
    38581 tatttcacat attagcaacc aaccagcaac atccttatgg cacaaaaata gaaggtcaat
    38641 acatcttatc tttcaggatt aaaaaatatg accacaccct cagatttgaa catttaccaa
    38701 ctgattgaca cccaaaatgg tcgggtcact ccgcgtattt ataccgaccc ggatatttac
    38761 caactggagc ttgagcgtat tttcggtcgt tgctggctat ttctcgccca cgaaagccag
    38821 atcccaaaac ccggtgattt ctttaacacc tacatgggag aagatgcggt tgtcgtagtg
    38881 cgtcagaaag acggcagcat caaggcgttt ctcaaccaat gccgccaccg ggccatgcgt
    38941 gtgagttatg cagattgcgg caacactcgc gcctttacct gcccgtatca cggctggtct
    39001 tatggcatta acggcgagtt gatcgatgta ccgctggaac ctcgcgccta cccacaaggg
    39061 ttgtgtaaat cccactgggg actaaacgaa gttccttgtg tggagagtta taaagggctg
    39121 atttttggca actgggatac cagcgcaccg ggcctgcgtg attacctggg tgacattgcc
    39181 tggtatctgg atggcatgct ggatcgtcgc gaaggcggca ccgaaattgt cggcggcgta
    39241 caaaagtggg tgatcaactg taactggaaa ttcccggcag agcagttcgc cagtgaccag
    39301 tatcatgctc tgttcagcca tgcttctgcc gttcaggtat taggggcgaa agatgatggc
    39361 agcgataagc gcctcggtga tggacaaacc gcccgcccgg tgtgggaaac cgccaaagat
    39421 gcgctgcaat ttggtcagga cggtcacggt agcggtttct tctttactga aaaaccggat
    39481 gctaatgtct gggtcgatgg cgcagtttca agctattacc gcgaaaccta tgccgaagca
    39541 gaacaacgtt taggtgaagt tcgcgccctg cgcctggcgg gtcataacaa tatattcccc
    39601 acgctttcat ggctaaacgg cactgccaca ctccgcgtct ggcatccgcg cggccctgat
    39661 caagttgaag tgtgggcgtt ctgtattact gacaaagccg cctccgatga agttaaagcc
    39721 gcttttgaaa acagcgccac tcgtgctttt ggtcctgctg gttttctcga gcaggatgac
    39781 tcggagaact ggtgtgaaat ccagaaattg ctgaaaggtc accgcgcccg caacagcaaa
    39841 ctgtgtctgg aaatggggct tggtcaggaa aagcgtcgcg acgacggcat tccaggcatt
    39901 actaactata tcttctcaga aacggccgct cgtggaatgt accaacgctg ggccgatctt
    39961 ctgagtagcg aaagctggca ggaagtgctc gataaaaccg ccgcttacca gcaggaggtg
    40021 atgaaatgag tgcgcaagtt tcactagagt tacatcaccg cattagccag tttctctttc
    40081 acgaagccag cttactggac gactggaaat ttcgtgactg gctggcgcag ctcgacgaag
    40141 agattcgtta caccatgcgc accacagtta acgcgcaaac acgcgaccgc cgcaaaggcg
    40201 tccagccacc gacaacctgg atttttaatg acaccaaaga ccagctggag cggcgaatcg
    40261 cccgtctgga aacgggcatg gcatgggcag aagagccgcc gtcacgcacc cgccacttaa
    40321 tcagcaactg ccagataagc gaaaccgaca tcccaaacgt atttgctgtt cgggtgaatt
    40381 atctgcttta tcgggcacaa aaagagcgcg atgaaacgtt ctatgtcggg actcgttttg
    40441 acaaagttcg ccgtctggaa gatgacaact ggcgcttgct ggaacgggat atcgtcctgg
    40501 atcaagcggt aatcacttcc cataacctga gtgtactgtt ctgatgaatc gaatttatgc
    40561 gtgtcccgtt gcggatgtgc cggagggtga ggctctccgg atcgatacct cgcccgtcat
    40621 cgccctgttc aacgttggcg gcgagtttta tgccattaac gatcgttgca gccatggtaa
    40681 tgcgtcaatg tcagaagggt atctggaaga tgacgccacg gtggagtgcc cgctacacgc
    40741 cgccagtttt tgcctgaaaa cggggaaagc gttatgcctg cccgccaccg atccgctcac
    40801 cacttatcca gtacacgttg aaggtggtga cattttcatc gacttaccgg aggcgcagcc
    40861 atgagcgatc tgcataacga gtccattttt attaccggcg gcggatcggg attagggctg
    40921 gcgctggtcg agcgatttat cgaagaaggc gcgcaggttg ccacgctgga actgtcggcg
    40981 gcaaaagtcg ccagtctgcg tcagcgattt ggcgaacata ttctggcggt ggaaggtaac
    41041 gtgacctgtt atgccgatta tcaacgcgcg gtcgatcaga tcctgactcg ttccggcaag
    41101 ctggattgtt ttatcggcaa tgcaggcatc tgggatcaca atgcctcact ggttaatact
    41161 cccgcagaga cgctcgaaac cggcttccac gagctgttta acgtcaatgt tctcggttac
    41221 ctgctgggcg caaaagcctg cgctccggcg ttaatcgcca gtgaaggcag catgattttc
    41281 acactgtcaa atgccgcctg gtatcctggc ggcggtggcc cgctgtacac cgccagtaaa
    41341 catgccgcaa ccggacttat tcgccaactg gcttatgaac tggcaccgaa agtgcgggtg
    41401 aatggcgtcg gcccgtgtgg tatggccagc gacctgcgcg gcccacaggc gctcgggcaa
    41461 agtgaaacct cgataatgca gtctctgacg ccggagaaaa ttgccgccat tttaccgctg
    41521 caatttttcc cgcaaccggc ggattttacg ggtccgtatg tgatgttggc atcgcggcgc
    41581 aataatcgcg cattaagcgg tgtgatgatc aacgctgatg cgggtttagc gattcgcggc
    41641 attcgccacg tagcggctgg gctggatctt taaggaagca cgatgaaaga aaaaaccatc
    41701 attattgtcg gtggcgggca agcggcggca atggctgcgg cctcgctacg ccagcaaggg
    41761 ttcaccggtg agctgcatct gttttccgat gagcgacatc ttccttatga acgacctcca
    41821 ctctcgaaat ccatgttgct ggaagattcc ccgcagttac agcaggtgtt acccgctaac
    41881 tggtggcagg aaaacaatgt tcatctgcat tccggtgtaa ccatcaaaac gctgggtcgc
    41941 gacacacgag agttagtgtt aaccaacggc gaaagctggc actgggatca gctttttata
    42001 gcaaccggcg cggcagctcg accgctgccg ttgcttgatg cactgggaga acgctgcttt
    42061 accctacgcc atgccggtga tgccgccaga ctgcgagaag ttctgcagcc cgaacggtca
    42121 gtcgtgattg tcggtgccgg aactattggt ctggaactgg ctgccagcgc cacgcagcgc
    42181 agatgtaagg tgacagtgat tgaactggcg gcaaccgtca tgggccgtaa tgcaccaccg
    42241 cccgtgcaac gctatctttt acagcgccac cagcaggctg gtgtgcgcat tctgctcaat
    42301 aatgccattg aacatgtggt cgatggtgaa aaagtagaac tgacgctgca aagtggggaa
    42361 acgcttcagg ctgatgtggt gatttacggt attggtatca gcgccaacga gcaactggct
    42421 cgcgaggcca accttgatac tgccaatggc attgtcattg atgaggcttg ccgcacctgc
    42481 gatcccgcga tctttgccgg tggcgatgtg gcaatcactc gtcttgataa tggtgcacta
    42541 caccgctgcg aaagctggga aaacgccaat aaccaggcgc aaattgccgc tgccgcaatg
    42601 ttagggctac cgctaccgct actgccgccg ccgtggttct ggagcgatca gtacagtgat
    42661 aacttacagt ttattggcga tatgcgtggc gatgactggc tttgtcgtgg caacccggaa
    42721 actcagaagg cgatttggtt taatctgcaa aacggcgtgc ttatcggtgc ggtcacgctg
    42781 aatcaggggc gtgagattcg cccaattcgc aaatggatcc agagcggcaa aacgtttgat
    42841 gcgaaactgc tgatagatga gaacatcgcg cttaaatcac tgtaaccagg ataattagcg
    42901 aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt gtattacgtt taaatcacat
    42961 tatcttgcaa agggattggt tatgaacaca ctacgttatt ttgattttgg agctgcccgc
    43021 cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa ttttcattat ttttggtttt
    43081 cccaaaatga tgggctttga cggtacggtc caatatatgg cctcgttggg cgcgccgatg
    43141 ccgatgctgg cagcgattat tgcggtagtt atggaagtgc ccgccgcgat attaatcgtg
    43201 cttggctttt tcacccgtcc gctggcggtg ctgtttattt tctacacgct gggtacggcg
    43261 gtgattggtc accattactg ggatatgacc ggcgatgcgg ttgggccaaa tatgattaat
    43321 ttctggaaga atgtcagtat cgctggcgca ttcctgctgc tggcaattac cgggccgggg
    43381 gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc ggcgtgaacg ccttatccgg
    43441 catttaataa attacagcca ctctacccgc aacgacatct ctgaagttgt tgattcccct
    43501 ggtgctagcg caatgagatc acccccttcc gggcgatgat gatcatccgg cgcatggctc
    43561 atcggttcca gacagaagaa atcaaacgca tatcctttat caaacgcagg gtcggaaaca
    43621 aagatgaaat aacacggtgc aggtggcgtc gtttccatga tgatggcata accttcctgc
    43681 ggctgctcga tgcgggcctg accattccat ccggcaaaac cattgttcac ccactggcgc
    43741 ggcaacggcg cgggctggtt aaaatccagt tcctgcggta gctgctcgca aaactcaccc
    43801 gccagccact gctcccgctc cagccagtaa ccgctcgcct gtgcctgaat ccgcgtttgc
    43861 ggcgacaacg gaaaataagg atgccagccg gtaccaaatg gcagcgtctc tgccccttga
    43921 ttggtgacag agagcgtcac cgtcagcgta tccgccgtta aatgaaacgc ctgacttacc
    43981 cgatagtgat agacaccgct gcgatgttca tacaccagac acaaactatc atcgctgtgc
    44041 gagacacatt gccactcgcc cagccagcca tcgccgtgta gatagtgcgc atcccactca
    44101 acattcggtt gcagttgata ctcacgcccc tgccagacaa aacgattgcc gctcacccgg
    44161 tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg ccacaccgct ttttttacca
    44221 ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga ttacgccgcc ctggtcagaa
    44281 acgtccagct ttagcgaccc gtgggataag gtatagatgg tcatccgcta actccttaat
    44341 ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc catcagcgca taggcatcac
    44401 ctgcctgttc cagcgagaag cgatgggtaa tggcgttacg cggccacagc ttccagtccg
    44461 tcagatcatg ggcgcatttt tccatatgga acagactggt cacccaggag ccgataatcc
    44521 gccgttgatg gtgcatcaga tcggcgctga cctcgaattc cacttttccg gtttcaccaa
    44581 tgtaaaccac ccgtccccag tcagcggtgg attgcagtgc cagcaagcga cctgcggcat
    44641 taccggaaca atcgagcgca acatccgcgc caccgtgggt gagttcggcg ataatctgcg
    44701 gcagaccttc ggtggttgct aaatagccgt gatccatcac ccctaactgt tttgccatcg
    44761 ccagacgttc cggcagcata tcaacgccga tgatccgttt tgcaccgcga cctttcgcca
    44821 gcatcatcgc catcatgccg accggcccca gaccgaccac cagcacgtta tcgctgccgg
    44881 aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac gccgcaactg ataaacgcac
    44941 catcttcgta gctcagcgca tccggcagga ggatcagatc tttttcttcc gccagcagat
    45001 attcggcatg accgccgtca cgctgccagc cgtaagccgc ttttcctttg ccagtacaag
    45061 agataggaaa gccgcggcgg cagttcgggc aaaaaccaca gccagaaatg tgatacacca
    45121 gcacgcggtc gccctcttta aaatggcggc agccttgccc catcgccaca atctgcccgc
    45181 acggttcatg accgttgata aatccctggt ataacggttt gtcgggtgcc gctgccgtag
    45241 cgcggtgctg gtggtagata tagtggacat cgcttccgca aattccggaa gatttcattt
    45301 tgatcagtac ctggttaatc cctggtgtcg gcaccgcaac ttcccgtaga tcgacggtcg
    45361 aatttcctgg taaataagct gccagcatcg ttttcataaa tccctcatta acaatacgat
    45421 taattttcat ccctgccgca cccgcgccag gggcgttaag gtttagcgtt tcgctttact
    45481 gcttcgctgg gtcagcaaga tattcgccag caccgccacc acgatgatga cgccgcgtac
    45541 cacctgctgg aaaaaggagt taataccgag cagcaccaga ccgttaccga ttagcgtaat
    45601 caccagcaca ccaagcaatg taccgaacag ggaaccgcga ccgccggaaa gtgccgtacc
    45661 gccgaccacg accgcggcga tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc
    45721 agaaccgagg cgcgccgcca acaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt
    45781 aaagataaga atgcgcaccc gacgaacgtt gatgccgcac aactgcgccg ccgtagcatt
    45841 accgccaacg gcaaaaaccg agcgcccgaa ggcggttttg cggctgatga acacaaacag
    45901 cgcaaacaac acaatcatga tcagcgcgga taccggcaca ccgagaaatt gtccgcccag
    45961 ccagtccagc acctcgtttt cgtcaatcgg cactggcagt gcgttcgtca taaacagccc
    46021 cattccgcgc agggcgctcc acagccccag tgtggcaacg aaacttggta cgttaaacac
    46081 gccgcgcagc accccggcga gcgttcccat cagcgcgcct aacagcaaca ccagcagaca
    46141 agcaatcgcc aacggaactt caaattgcag caaaaatgcc aggcacaccg agacaaaagc
    46201 caccatcggc ccaacgctga catcaatctc accggagata ataatcagcg tcatcgccca
    46261 ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc atctggttat tcaatgagat
    46321 aaagccaggc gcgttcaggg agaagaccag ataaagaatg gcaatcacca ccagcaaacc
    46381 gatctcatta atatggcgac tgacaaattg tttgagcgag acgctcttgc cctgcggcaa
    46441 tggtaatgac gaagcagaca tcttcaggtt cctcggtaaa attcatcagt gcacagacag
    46501 aatggcggac atcagctcat ccacattgac cggcgagtga aactcctgcg agaacgtacc
    46561 gtgctgtaac aacaggatgc ggtcgcacac cagcggcaac tcctccactt cactggagat
    46621 aaacaccacg ctttttcctt cggcagccag ctcacggaca atacggtaaa tctgctgttt
    46681 ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac aaaatctggc tggcggcata
    46741 gacccaacga ccgatcacca ctttttgctg attgccgcca gaaagcgtgc cgatgggcgt
    46801 ttcgctactg gcggccttga ccgtcatccg ctgcatcacc tcttcggtca ggcggcggat
    46861 ggtggaccat tgcagtacgc cgttggcgct gattttttgc cgattggtca gcactgtatt
    46921 ttcgtcaacg cccaaccagg gaatgatccc cgcttctttg cggttttctg gcgtatagcc
    46981 aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcgtg attttctcgc cgttgataac
    47041 aatttcgccc tgttcatact cctccagccc aacaatcgcc ttcagcaatt cactgcgccc
    47101 tgcccccagc aggccagcaa tgccaagcac ttcgccgcga cgtaacgtaa agctgatatc
    47161 ctccagcttg ggcttatggc gtaatgcacg gacttccagc acggcctgat cgacaatttc
    47221 ctgaggggct accggcgcaa tatcaacgtg atcgcgccct agcattagcg acacaatatg
    47281 atgcgtggag gtgttttcga gcatcacatc gcccgccacc tgaccatcgc gcataacggt
    47341 ggcacaggag gcaatgcggc gaatttcttc catccggtgg ctgacataaa tcaccgccac
    47401 gcccagtgcc gacatctttt tcaccgcgct gatcaccagt tcaacttccg cactggcaag
    47461 cgaactcgta ggttcatcaa gaatgaccac gcgcggctcg cccttcatca cccgtgcaat
    47521 ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca agttgttcag gactaacgtc
    47581 aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc atttgcaggt aatcaatcat
    47641 gccgttgcgg cggggccact gaccgaggca gaggttttcc gccaccgtca gcccttccac
    47701 cagacttaac tcctgataaa ccgcacgaac ccccagttca gcggcacggc gagtcaacgt
    47761 agcttcgtca ccttccagtc gcgtctcgcc aatccagata tcaccgctat ccgggcgttc
    47821 actgccggta agcattcgaa tgagagtcga tttgcccgcg ccgtttttac ctaacagcgc
    47881 acgaacttcg cctttattga gcgtgaagtt aacgttatcc agcgcaacga cgccggggta
    47941 acgcttattt cctgccacca cttttgctac cgggactgcc tctgttgccg tgaacatagg
    48001 gacctctgcg aatcagcgat tagggcagac catcaacgtg cgttgccagc cactgtttac
    48061 catcttccgt tttggtatag agatcgattg gcacctgaat cactttttca ccgtcggctt
    48121 gtttattgat aaccttcaat gtttgcgcga gaacagcatt gcccattttc ttaccggaaa
    48181 tatccactac cgctttcagc acctgattgt tttccagctc ctgagcaatt tcagtggtca
    48241 tatccgaacc gaaaacagca atttttccgg cctgattttg attacgtacc gcttttaccg
    48301 cgccgagtgt cgctccgccc gattcaccca taatggcgtt gagatccggc gtggagataa
    48361 ttagtttttc accaacggaa atcgctttat ctaaaacagt cccttcctga ttagcgacaa
    48421 tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa tcctttacgt cgctgcacac
    48481 aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg ctggtcaatt ttattggcaa
    48541 taaaataatc ggcggcagca ttacccagtt ttttaccaaa ttccagcgga tcgccgacca
    48601 gatacgccga gacatattta tcgacaccct tttgattaat acaggtgttg tagcaaatca
    48661 ccggaatacc cgcttcactg gcgcgacgaa cggtacggct actgccattt tcagacactg
    48721 ccgataaaat aatggcatcg acattacgcg ccacgagggt atcaacaaag gtactttctt
    48781 tcgaaatatc accctgggcg ttagtttcaa ttaactgtac ctgtactgaa gaatcttttg
    48841 ccgcatcctg aacgccttga cgcactccag cgtaatatcc ctgagtatca aggtatattg
    48901 cgccaatggt catttctttt tccgcagccc tggcaaataa agcgctacct aatagcgtag
    48961 ccattaataa tagattacgg gttgttctca tttttttagg catagagcct cctgtagggt
    49021 ttttattaac aacggcttat tctaattatt ttgtgatgag cggcagcgcg tgccgctcaa
    49081 atattacagt taatagggtt tagtgaacgt agttaaaaat aaaaggcatc acagtggtga
    49141 ataatgccgc tttcggccag gccggattaa tcgccgtcaa ttcagcgcga gcagactcat
    49201 attgcgcaac ctccccgagt cccgcggacg ccagcatcgc caccatcagg catttttctt
    49261 tatgctgctg ttgtaaatcg ccatacagcg ataacaggtc aggttgtgaa acagcaaaga
    49321 aatccgcctc aatactggtt ttcgccatct cttgcgccca ctgtttcatt tcactaaaca
    49381 gttgctgtgc ggtgtgttgt tcgcccagca gtcgcagcgc cattccttgc cagaagagat
    49441 aatcaaccgg ctgatcgttg taataactgt ggatgttaat ggtgcgatcg ccggtcgccg
    49501 ccagacgtaa acaacgcatc gcttcagttt catcgccctg cgcgttggcg catatcgcct
    49561 gccagaacca gatgtcgtta tcagtttgcc ccggtaaacg gccttcgctt aaattctccg
    49621 gataatgcag cgcggcatga agcagttcgc tggcctgctg cggctgtctg gcatcaaggt
    49681 gctgccaggc gcgtaataac tggttgagga taaactgact ggtgaccttc ccttccccgc
    49741 cttcccacgg gtggaatttg cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg
    49801 tgagatgcca caaattgagc aactctgctg tcatgtcgtc gcgtttcagc gcaatttcca
    49861 gattattttc cagacgcgcc agtcgtttct ccggtgtggc tccgcttaac ttatcaagca
    49921 aatcccgttc gaaaagcaga cgtgcatcct gcggcgcaag ctgataagca ttatcaagat
    49981 aacgcgcggc cagctcataa tcgtgttgct tattccacgc atggatcgct aacccgcgcc
    50041 agccgtcggc aaactccggc gacatctcta cgcaacgttg ccataaggta atggctttgt
    50101 tgtagctacg cttgttgtag tagaagcagg ccagtaaatg acgggcaaac cagcactctt
    50161 caatactctc cagcgccgcc acttcttcca gcgtattcgg gaaacggaca aactgcggga
    50221 agacatcaat ggcttttgcg accagttcgc cacgttcggc tttcggcagc aagctggctt
    50281 gcaggtaaag cggcagcgtg cgctggcagt ccagtgcgtt cagcatctct gccgccaggg
    50341 tgggcattcc ccagttaatc agttgcccgg cggtcatcag ggcgttaacg tcgcgtccct
    50401 gacacagacc gcgccactgc gcgagagctg attcgctacg accatcgaac cagttcagcc
    50461 accacagagt ggcgttcagc ggataatcgc gcagcagttt ctcgcgctgc acacgcgcgt
    50521 tgtcctgacg accacttaac accagcagca gattatgcag gcaaagcact tcctgattgg
    50581 ttgggcaggc gcgaagactt tgttggcaaa aatccagacc agcgtcgaag ttaccattac
    50641 gcgccgccag tcgtgccagg ccataatagc caccggcttt gctgttgccg ctccagaccg
    50701 cacgccagaa atcctcttcg gcttgttgat attgtccctg acgttcgtaa gcactggcgc
    50761 gaatcaaact cgcctgtccg cactgcggat ttttgttcag cgcatgtgcg cgtttcagag
    50821 cctgagtggc atacgccacc gcttgcggga aatctgcgcg gttatattcc agcatcgcca
    50881 gcgccaggtt acagcgataa tccagcgggt ccagcgccac gccgcgcagg tagtaatcga
    50941 acggtgaacg gctggcgtga tgatattgct ccagatgctg accgataaac caggcttcat
    51001 ctgtactggt aatgtcttgt gctgccagtg gcgctttggc gacgtccggc aacggcaact
    51061 cttgcggctg atgttcctga taactcagga caatattgcc gtcggcatcg gagagctcaa
    51121 tggtcagtct ttcaggattg ataccgtgca acacgccctg gatggcggtc gcaggcatca
    51181 gtgccacggc atcatcaagt aacgcgttgc atttgccgat ttcgcggatc gccaggcggt
    51241 atccgttcaa cggagagatg gcatacagcc cccactcaat cccccgctca ctacgctgga
    51301 gttttatcac cgcatcgcgg gaggcatttt gcaccatgcc caaagaatga taaggcagga
    51361 aatactgctc aaaacgcttc tcttcgtaag catcaagcca ggtaaaatca ggctggttat
    51421 cggcaaaaat accggtcatc agttcgatat acgggccgtt attgtcggtc agactcttgt
    51481 cccacgcctg gccgaattca ctgtgtcccc agctccactg ttttttacct ggcgcgatat
    51541 ggtggttggc aacgtgtagc aaaccgccat cttcatcgtg acaccacgcg ccaacaaaat
    51601 cgtactgtga tttttcagcc atatatgagg ttggaacggg cacattttta tagcgagaaa
    51661 tgtccactcc ggcggagtag tccactttgt agtaagtgcc ggtggcgatg gggaaagcgg
    51721 agacggcccg tttgccgtga tcaaacaccg ccgttacatc cggcgggaag acgctctgat
    51781 gcccttcccc ccctttcact gccgggttgg cccaccacaa gaaatgacgc ggcgtggcgt
    51841 tgccgttata gacgcggctg gcgatttcca acgccgcccg ttcaggacgc agtgtgaaac
    51901 ctgtcatcac ctgtaaaccg tgcatcggct ccgtttcgcc gacccacacc gtctgtgcgc
    51961 cgtcttcatg ggcttcgggg gtgaaatcaa cgggcataaa ggtggtcggg cgatggtgtt
    52021 gcggccagtt aaactcaatc ccgccagaga tccacggtcc cagcagcccc accagcgcag
    52081 gtttaatgac ttcattgtga tagacaaaat cgcgttgttt cactttatcc catgcgcgat
    52141 gcacacgacc gcccagttcc ggcaggatca tcactttgat gtagtcgttt tccagccaca
    52201 ccgcctgcca ggatttcagg gttttctgct cgctcagcgt atcggtcacg ccgtagggat
    52261 aaaccgcgcc ggacgatccc tgataaacgc gattttccag gaacatggga tgtatatcct
    52321 gcggcccggt ttcataggtc gggatctcaa cgcgctcttg ccacactttt actggagtca
    52381 tggtgccctc aatattaaca agacatactg aattaaaaga tttgtggcag tgtattgaac
    52441 aatctggcaa tgttttcgcg gaataatcac gcaattaact aaacaaggtt tagtgaagat
    52501 gagagcctgc attaacaatc aacagattcg ccaccataac aaatgcgtga ttctggaact
    52561 gctgtaccgg caaaagcgcg ccaataaatc aacgctggcc cggctggcgc aaatttcgat
    52621 tccggcagtc agtaatattt tgcaggaact ggaaagcgaa aaacgggtgg tgaatattga
    52681 cgatgaaagc cagacgcgcg ggcatagtag cggtacatgg ctgattgcgc cggaaggtga
    52741 ctggacgctg tgcctgaacg tgacgcccac cagtattgag tgtcaggttg ctaatgcttg
    52801 tttaagtccg aaaggtgaat ttgagtattt acagattgat gcaccgacgc cgcaggcgct
    52861 gctgtccgaa atcgaaaaat gctggcatcg ccaccgtaaa ttgtggccgg accgcaccat
    52921 caatctggcg ctggcaatcc acggtcaggt tgatccggtg accggcgtgt cgcaaaccat
    52981 gccgcaagcg ccgtggacaa cgccggttga ggtgaagtat ctgctggaag agaagctggg
    53041 cattcgggtg atggtcgata atgactgcgt gatgctggcg ctggcggaga aatggcaaaa
    53101 taattcgcag gaacgggatt tctgcgtgat caacgttgat tacggcattg gctcgtcgtt
    53161 cgtgattaac gagcaaattt atcgcggcag tttgtatggt agcggacaga ttggtcacaa
    53221 catcgttaat ccggatggcg tcgtctgcga ctgtggacgt tacggctgcc tggaaaccgt
    53281 cgcctcgtta agtgcattaa aaaaacaggc gcgggtatgg ctaaaatcac aaccggttaa
    53341 tactcagctt gatcctgaaa aactgacaac cgcgcagtta atcgctgcct ggcagagtgg
    53401 agaaccgtgg atcaccagct gggttgatcg ctctgccaat gccattggtt tgagtctgta
    53461 taacttcctc aacatcctca atattaatca gatttggatg tacggtcgca gttgtgcctt
    53521 tggtgagaac tggcttaata ctattattcg ccagacagga tttaacccgt tcgaccgcga
    53581 cgaaggaccg agcgtgaaag cgacgcaaat tggcttcggg caattaagcc gcgcacaaca
    53641 ggtgctgggg attggctatt tgtatgttga ggcgcagtta cgacagattt gatggcgcga
    53701 taaccgcgat aacgtagaaa ggcttcccga aggaagcctt ttttcaacag attaaccgtt
    53761 tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag aactttacct ttgatgcgct
    53821 cgataacggc ttcatcattg atgctgtcca gcacgtcaca catccagcca gccagttctt
    53881 tcgcttctgc ttctttgaag ccgcgacgcg taatcgccgg agtacccacg cgaataccgg
    53941 aggtcacaaa cgggctcttc ggatcgttcg gtacgctgtt tttgttgacg gtgatgttag
    54001 cacggcccag agcggcgtct gcttctttac cggtcaggtt tttatctacc agatcaacca
    54061 ggaacagatg gttatcagta ccgccggaaa ccactttgta gccgcgttcc aggaacactt
    54121 ctaccatcgc tttagcgttt ttagcgacct gctgctggta agttttgaac tcaggctcca
    54181 tcgcttcttt cagagcaacc gctttaccgg cgattacgtg catcaacgga ccgccctgac
    54241 caccagggaa aacggcagag ttcagttttt tgtacagctc ttcgctacca cctttcgcca
    54301 ggatcaggcc gccgcgcgga cccgccaggg ttttgtgagt ggtggtagta acaacgtgag
    54361 catgaggaac cgggttcggg tagacgccag cagcaaccag gcccgcaacg tgcgccatat
    54421 caacgaacag gtaagcaccg atgctgtcag cgatttcacg cattttcgcc cagtccacca
    54481 cgccggaata tgcagagaaa ccaccgataa tcattttcgg cttgtgttct ttggcttgtt
    54541 tttccagatc ggcgtagtcg atatgaccgg tagcatcgat accgtaagga acgatgttgt
    54601 acagtttacc ggagaagtta accggagaac cgtgagtcag gtgaccgcca tgcgccaggt
    54661 tcatacccag aacggtatcg cctggttcca gcagcgcggt gtagaccgca aagttagcct
    54721 gggagccgga gtgcggctgg acgttagcgt agtcagcgcc gaacagttct ttcgcacgat
    54781 cgatcgccag ttgttcaacg atatcaacat actcgcaacc gccgtagtag cgtttgcccg
    54841 gataaccttc agcatatttg ttggtcagct gagaaccctg cgcctgcatt acgcgcgggc
    54901 tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc ctgacgtact ttttcctgct
    54961 ccatagcctg ccacagttcg gcatcataat cggcaatgtt catttcacgc tttaacatcc
    55021 gcatctcctg actcagctaa caataaaatt tttggccttt ataggcggtc ctgttggaca
    55081 acggcgaaca gtataaccga atcattgtgc gataacaggt cttgacaaag gaatttacgc
    55141 aaacgattac cttcaggcta cgcaaggctt tggagaataa agagcttgca accggaaacg
    55201 gatttctttt caggtttgtg atgcaaattt ttcacttcat cacattcttt ctgaaaaaca
    55261 ccaaagaacc atttacattg cagggctatt ttttataaga tgcatttgag atacatcaat
    55321 taagatgcaa aaaaaggaag accatatgct tgacgctcaa accatcgcta cagtaaaagc
    55381 caccatccct ttactggtgg aaacggggcc aaagttaacc gcccatttct acgaccgtat
    55441 gtttactcat aacccagaac tcaaagaaat ttttaacatg agtaaccagc gtaatggcga
    55501 tcaacgtgaa gccctgttta acgctattgc cgcctacgcc agtaatattg aaaacctgcc
    55561 tgcgctgctg ccagcggtag aaaaaatcgc gcagaagcac accagtttcc agatcaaacc
    55621 ggaacagtac aacatcgtcg gcgaacacct gttggcgacg ctggacgaaa tgttcagccc
    55681 gggccaggaa gtgctggacg cgtggggtaa agcctatggt gtactggcta atgtatttat
    55741 caatcgcgag gcggaaatct ataacgaaaa cgccagcaaa gccggtggtt gggaaggtac
    55801 tcgcgatttc cgcattgtgg ctaaaacacc gcgcagcgcg cttatcacca gcttcgaact
    55861 ggagccggtc gacggtggcg cagtggcaga ataccgtccg gggcaatatc tcggcgtctg
    55921 gctgaagccg gaaggtttcc cgcatcagga aattcgtcag tactctttga ctcgcaaacc
    55981 ggatggcaaa ggctatcgta ttgcggtgaa acgcgaagag ggtgggcagg tatccaactg
    56041 gttgcacaat cacgccaatg ttggcgatgt cgtgaaactg gttgctccgg caggtgattt
    56101 ctttatggct gtcgcagatg acacaccagt gacgttaatc tctgccggtg ttggtcaaac
    56161 gccaatgctg gcaatgctcg acacgctggc aaaagcaggc cacacagcac aagtgaactg
    56221 gttccatgcg gcagaaaatg gcgatgttca cgcctttgcc gatgaagtta aggaactggg
    56281 gcagtcactg ccgcgcttta ccgcgcacac ctggtatcgt cagccgagcg aagccgatcg
    56341 cgctaaaggt cagtttgata gcgaaggtct gatggatttg agcaaacagg aaggtgcgtt
    56401 cagcgatccg acaatgcagt tctatctctg cggcccggtt ggcttcatgc agtttgccgc
    56461 gaaacagtta gtggatctgg gcgtgaagca ggaaaacatt cattacgaat gctttggccc
    56521 gcataaggtg ctgtgatttg atgttgccgg atggaaacat ccggcaaccc ttgacgcggt
    56581 ttaaattgcc gcgtcgtcct cttcaccggt acggatgcga atgacccgtg ccacgtcaaa
    56641 gacgaagatt ttaccgtcac cgattttgcc ggtttgcgcc gtgcgaataa tggtatcgac
    56701 acaggtatcg acaatgtcgt ccggtacgac aatctcaatt ttcactttcg gcagaaaatc
    56761 caccatatac tccgcgccgc ggtacagctc ggtatggcct ttctggcgac caaagccttt
    56821 cacttcggtc accgtcatgc cggtaatacc gacttcggcc agtgcttcgc ggacatcgtc
    56881 cagcttgaag ggttttataa tcgcatcaat ctttttcatg ctattccttg aaaaggtcgc
    56941 ctgtcttttg atctgctaaa cgtaacacat aacgccaatt cattccttga aatcgtttgc
    57001 atccagctcg tgtcgggaaa gcagtttata aaattctgtc cggttgcgcc ccgccattct
    57061 cgccgcgtgg gtgacgttgc ctttggtgat ttgcagcagc ttacgcaaat agttgagttc
    57121 aaactgatta cgtgcctcaa caaaggttgg cagcgccgta ttttcaccct ccagcgcctg
    57181 ctctactaat gcatcgctaa taaccggaga tgaggtcagc gccacgcact gttcaatcac
    57241 gttgaccaac tggcgcacat tacccggcca gctcgcggtc atcaggcgtt tcatcgcatc
    57301 ggtagagaac gcgcggacaa acggtttatg tcgctctgcc gcctggcgca acaggtgatt
    57361 tgccagtagc ggaatgtctt ctgtgcgctc cgccagtgcc ggaattttca ggctgacaac
    57421 gttgaggcgg taatagagat cttcgcggaa ttccccgcgc gccatcgctt ttggcagatc
    57481 acggtgagtg gcagaaataa tccgcacatt gatatcaata tcgcggttac tgcccagcgg
    57541 gcgcacttta cgctcctgca acacgcgcag cagtttgacc tgtaacggcg caggcatatc
    57601 gccaatttca tcgagaaata gcgttccgcc ttccgccgcc tggaataaac cttcgcgatt
    57661 gctgacagcg ccagtaaacg cgccacgcgc atgaccaaac agctccgact ccagcaattg
    57721 ctcgggcaat gcgccacagt taatagcaat aaatggtttg ctgttgcgcg ggctggcgtt
    57781 gtggatagcc tgggcgaaaa tctctttccc ggtgccgctc tgaccgttaa tcaaaacgct
    57841 gacgtctgat tgcgccacca gccgcgcctg ttccagcaaa cgcagcatca gcgggctgcg
    57901 ggtgacaatt gcctcgcgcc agcgttcatc ggtggctggc gcggattgct ccagcgcatc
    57961 gtcaattgcc tgatatagcg cgtctttgtc gacaggcttg gtgaggaaac taaaaacgcc
    58021 ctgctgtgtt gcagcaacgg catcggggat agaaccatgc gcagtaagaa taattactgg
    58081 cattcccggc tgcactttct ggatttcagc aaatagctgc ataccgtcca tttcatccat
    58141 ccgcaggtcg ctgataacta aatctacttt ttcgcgattc agtacccgta atccttcagc
    58201 gccactttcc gccgtgacca cactgtagcc ttcgctggtc aggcgcaggc caagcagttt
    58261 cagcaatccc ggatcgtcat cgaccaataa taaatgcgca ggtttatggc tcatcaggag
    58321 tgacctcatg ggtggatggc gcgggctttt cactctcgtg cggcgtatcc ggcgagaaat
    58381 ttccagccgg tttgcgggtc gagagctggc gttcaatatc ggtcaggttt tccagcttgc
    58441 gggtggtgag ttccagttgc tgctgtagaa cgtgatgttg ctggcgcaat gtatccagct
    58501 cgctgtcgct ggactgctgg agtttactgt aacgttggcg ctcttccgcc agttgcagtt
    58561 gcagcgcctg accgtcgcgc cagagttgat acagtgggcg aacctgtgcc gggatctcgg
    58621 tacttaacgc ttcaatacgc gcgaccagct ggcggcgctc atacggcgta attttggcgt
    58681 cggcgagcaa aatcccttgt ttaaaggtat tttgccagct gccgtcgtca tattggcgag
    58741 cttgctgacg cgactgcgca ggcattaaac gatcagcaca atccatcgcc cgcagccagt
    58801 aaagcggatt ggtttcggtt gatttacctt gcagcgccca gatgtcgcta cattcagtag
    58861 aaagatagtc agccagttga taaaccggaa ttttttcttc tgctggcgta tcaatggctg
    58921 gcttattgtg attctgcacg caacccagca atgccagaca tggcagccct gccagccaca
    58981 gccgtcgggg caataatcgt tgaaaaatgt gtcgcatatt caccagactt aaagcctatc
    59041 ccagtgggcg taattgttgc agacagtctg gacatggaca gcgcggagaa accggagcgt
    59101 acatatcata cgtgaagatt tcgagcactg cccggggccg aactgacaaa taaaatagcc
    59161 tgataggata ggctgttagc attatttcgt gtttttcgac gacggtaatt caatgcggaa
    59221 acaaacgtct tgcccgctct cgtcgaccag atacagttcc ccttgcatac ggcgaataca
    59281 atccctggca atgcttaatc ccagaccgct gcccttcacc gccccttttc gctggtggct
    59341 tccctgaaaa aagggttcga agatcatggc gcgttcctct tgcggaatgg gcgtgcctgt
    59401 attgatgaca tcaatataaa cccgcgcacc atgtgaactg ctgcgaaggc aaatgttacc
    59461 ggattcagcc ccgtagtgca ccgcattgga gtaaagatta tccagtacgc tcatcagcag
    59521 cattggctcc gccaggcaag ctgttgcttt gagatcgacg tcggtatgca tcattttagc
    59581 ccgtgcgggc aggctatgag cagaaaccac tgtctccacc agcggtgcta attcaacatt
    59641 ctccagttcc accgcactgt ccgcctgttt acggttgtaa tcaagcagtt gttcgatcag
    59701 tttttgcaaa ttgcggctgc tgctatcaag aatgctcacc acctcttttt gctctggcgt
    59761 aagcggcccg acaacctggt cagccagtaa ttcagtgccc tcgcgcatac tcgccagtgg
    59821 cgtttttaat tcatgagata aatgtcttaa aaattgatgg cgttgggatt ccagccatga
    59881 caggcgctca cttaaccaaa gaatacgttg cccaaccgag cgtaactcgc tcggtccact
    59941 gaacgaaacg ctattgccca gagaacgccc ttcccccaga cggttgatca tgcgctcgat
    60001 atttttcacc ggcccgataa tcatccgcgt gaaaagtagc accatcacca gactcaccag
    60061 aaatagcacc agcgattgcc aaccaaaata ttgcccacgt tcggcgattt cacgctgaag
    60121 ttgctgccca cgagagaaca ccactgtgcg cgtggcctgc accatttcgg tattggcact
    60181 ggcaaaggct tcaagacgcg cggcggcggc agcatcggga ccactgttgt tacactgaag
    60241 ttgggccaga ttgttcaaat cctgacgcaa tgcctggtaa agtttatcgt ctggcagtac
    60301 gcctgcgtgg gcatcgagca tttcgctgta acgcttgcgc tggctttgat aaaccttcgc
    60361 aagcgtaggg tcgtccagca cgcaatactg acggtagcta cgctccatct ccagcgccgc
    60421 gttggtcatc gcttcactac gccgggcatc aataagcgta gtgcggttaa ccagcgccgc
    60481 ctgatcgcta agcgcattca ggctttgcca ggcttgccat gccagcacca acaagggcag
    60541 cagaattagc aaaaatgcca gcattaccag ttgtcgtaat gagcggggaa aaacgggcca
    60601 gcgtttcaag gtgttactct cgtcagacgc gaatagcctg atgctaaccg aggggaagtt
    60661 cagatacaac aaagccggga attacccggc tttgttatgg aataaggcgg tgcctaactc
    60721 gacgtttcgc ccgatggttg atatagctac gctgatatca gaagttggac ggcaggcacc
    60781 ttgttgtgcg tcattcgaat tttatgtagc acgtcccgaa ggggctgaca taagtcggtg
    60841 aatgagccac tgcttactat tatgcagtaa ctgtgccaat aaagaaaata gtttggtaac
    60901 gtactgatta tacgttgctt tgagggattt atggcttctt cgctatttga atgtaaatca
    60961 gccactgtgt cgccaaaaag agacaactta agataaactt attagattac aataaaatca
    61021 atcatttaag tgtcgctaga aagcgacacg gtaaaccacc cattgtcgtg atttacagac
    61081 acaaaaaagc tcccggagtt gggagcttat gatagtggtt ggtgcttaat gccgcatccg
    61141 ggctgcaaaa ccaatgggct gacgacttac cccaactgct tacgcgcatt gcggaaaata
    61201 cgcatccatg ggccatcctc gccccagttt tccggatgcc aggagttgct gacagtacgg
    61261 aaaacacgtt ccgggtgcgg catcataatg gtgactcgac cactttcagt cgtgactgcc
    61321 gtaataccgt tcggtgaacc gttcgggtta gccgggtagg tttcagtgac tttgccgaag
    61381 ttatcgacat agcgcagtgc caccagccct ttgctttcca gtgccgccag atgcgccgca
    61441 tcacgcactt ccacgcgccc ttcaccatga gagacagcaa tcggcatttg cgagcccacc
    61501 atcccctgca acagcagcga cgggctttgg gtcacttcaa ccaggctgaa acgcgcttca
    61561 aagcgatcgg aggtattgcg cacaaaacgt ggccacaact cactacctgg gatcagttcg
    61621 cgcaggttag acatcatctg gcaaccgtta cataccccca gcgccagcgt ttgcggacgg
    61681 tggaagaagg ttgcaaactc atcgcgtaca cggtcattga acaggattga cttcgcccaa
    61741 ccttcaccgg cacccagcac atcaccgtag gagaaaccac cgcacgcgac cagggcgtgg
    61801 aaatcttcca ggcccgtgcg tccggtcagc aggtcactca tatgcacgtc gatagcatca
    61861 aagcctgcac ggtggaaagc tgccgccatt tcaacatgcg agttcacgcc ctgctcacgc
    61921 agtacggcaa ctttcggacg tgcgccagtg gcaatatacg gtgctgccac atcttcgttg
    61981 atatcgaacg acagttttac attcaggcca ggatcggcat cgttagattt cgcctggtgc
    62041 tcctggtcag cacactccgg gttgtcacgc aggcgctgca tctgccaggt agtttctgcc
    62101 caccagacac gcaacgtggt gcggctttcg ctgaatacag tctgcccgtt ggcggtaatc
    62161 acaaaacggt caccggaaac cgcctgccct acataatgga cacaatcagc aagcccatgc
    62221 tgtgccagta cggactcaac cgcttcacgg tcagcggcac gaacctgaat caccgcaccc
    62281 agttcttcgt taaacaacgc cgccaggcga tcgtcgccca gagaggcaat atccgcgtta
    62341 atgccgcaat gaccagcaaa ggccatttcc gccagcgtta ccagcaggcc gccatcagag
    62401 cggtcgtggt atgcgagcag cttacgctgt gcaaccagcg cctgaatcgc gtcatagaag
    62461 cctttcagtt gcgcgacgtc gcgtacatct gccggtttgt cgccaagctg acgataaacc
    62521 tgcgccagcg ccgttgcgcc cagcgcgtta ttgcctttgc ccaaatcaat cagcagcagt
    62581 gcgttatctt cggtagaaag ctgtggcgtg atggtgtgac gcacatcttc cacgcgggca
    62641 aatgcagaaa tcaccagcga cagcggcgac gtcatttcgc gctcttcgtt accttcctgc
    62701 cagcgggttt tcatcgacat agagtcttta cccaccggga tcgtcaggcc cagcgccgga
    62761 caaagctctt cgcccacggc tttaacggct tcatacaggc ccgcatcttc accagggtgg
    62821 cctgccgccg ccatccagtt ggcggaaagt ttaatgcgtt tgatatcacc aatctgtgtt
    62881 gcggcaatat tggttaacgc ttcaccgacc gccagacggg cagaggcggc gaaatccagc
    62941 agcgcaaccg gcgcacgctc gccaatcgcc atcgcttcac cgtagtagct gtcgaggctg
    63001 gcggtagtga ccgcgcagtt agcgaccggc acctgccacg gccccaccat ctgatcgcgc
    63061 gctaccatgc cggttacgct gcggtcgcca atggtcacca ggaaggtttt ttccgccaca
    63121 gtcggcagat gcagcacacg tttcaccgca tctgcaatgg tgatcccttc acgggccagc
    63181 gcgtcgcctt tcgctttcag cgtttgtaca tcgcgggtca tcttcggcgt tttaccaagc
    63241 aggacgtcca gcggcagatc gatcggctga ttatcaaaat gacgatcgtg cagagaaaga
    63301 tgcagttctt cggtcgcttc accaatcacc gcgtagggtg cgcgttcacg cttacacagt
    63361 tcgtcaaaca gcggtaattg atcggcagca atcgccagca cgtagcgttc ctgggattcg
    63421 ttacaccaga tttccagcgg gctcatgccc ggttcgtcgc ttagaatatc gcgcagttca
    63481 aatttaccgc cgcgtccgcc gtcgctcacc agctccggca tcgcgttaga aagaccgccc
    63541 gcaccgacgt cgtggataaa cagaattggg ttagcatcac caagctgcca gcaacggtcg
    63601 atcacttcct ggcaacgacg ctccatctcc gggttgtcgc gctgtacgga agcaaagtcg
    63661 aggtcggcat cagactgacc tgacgccatg gaagacgccg ctccgccgcc aagaccgatg
    63721 ttcattgccg ggccgccgag aacgaccagc ttcgcaccga cgttgatctc gcctttttgt
    63781 acgtgatcgg cgcgaatgtt gccgatcccg cccgccagca tgatcggttt gtgataaccg
    63841 cgcagctctt cgccgttgtg gctgttcact ttttcttcat aagtacggaa gtagccattc
    63901 agtgccggac gaccaaattc gttgttaaac gccgcgccgc ccagcgggcc ttcggtcatg
    63961 atgtccagcg cggtgacaat gcgctcaggc ttaccgaaat cttcttccca cggctgttcg
    64021 aagccaggaa ttcgcaggtt ggaaacggag aaaccaacca aacccgcttt cggcttcgcg
    64081 ccgcgcccgg tggcaccttc atcgcggatt tcaccgccgg aaccggtcgc cgcgcccggc
    64141 cacggagaaa tcgccgtcgg gtggttgtga gtttcgactt tcatcagaat atgcgccggt
    64201 tcctgatgga aatcgtagcg gcccgtttcg tggtcagcaa agtagcggcc cacttcagaa
    64261 ccttccatta cggcggcgtt atctttataa gcagagagaa cgtgatctgg cgtggtttcg
    64321 aaagtatttt tgatcatcct gaacagcgat ttcggctgtt gttcaccgtc gataatccag
    64381 tcggcgttaa aaattttgtg gcggcagtgc tcagagttcg cctgagcgaa catatacagc
    64441 tcgatgtcgt tcgggttacg accaagcttt gtgaaagcat cctgcagata gtcaatttca
    64501 tcttccgcca gagccaggcc aagacgcagg ttagcgtcga tcagcgcctg acggccctgc
    64561 cccagcaaat caacgcttgt gaccggagtc ggttgatggt gggcaaacaa ctgctctgca
    64621 tcatctaaat caaaaaagac cgtttccatc atgcggtcgt gcagttcagc ggtaacctgc
    64681 tgccattgtt cattggtcag cgtaccggct tctatatagt aagcaacgcc gcgctcaagg
    64741 cggtttacct gttgtagccc gcagttatgg gcaatatcgg tcgctttcga agaccaggga
    64801 gagatggtgc caggacgcgg ggtcaccagc aggagtttgc cttgcggggc gtggctggcg
    64861 agtgccggac catatttcag caggcgttca agttgtgcgt gctcatcatc gtttaacggc
    64921 gcattgaggt cagcaaaatg gacatactcg gcgtaaatat tgtgaaccgg gagcctggca
    64981 gcctgaaaac gtgccagcag tttgttgatt cggaatgccg acagtgcagg cgaaccacgc
    65041 agaatttcca tcataagtct ctcgtcttct aagctttcgg tgtacccaag ggggggaaac
    65101 gggcgtcatt ataaagaatc tgatgcgctg acgaaaccgt ttgcgtggaa ataaaatcac
    65161 catcgtgaat tagcaacgcg tgccgccaat ggatgtaata agttgccatc tggcgcaggt
    65221 ttacgcaaaa tgccgctcat ttatgagtaa acctttcact attattacgt tttttcaagc
    65281 tgggacgcgc acgacacaga gaattaacta attgaaaaaa ttaaagatta attatctgtt
    65341 catcggcatt ctggcactgc tgctcgcggt cgctctctgg ccatccattc cctggtttgg
    65401 taaagccgac aaccgtatcg ccgccattca agcgcgggga gagttgcgtg tgagcaccat
    65461 tcatactccc ctgacttata acgaaatcaa cgggaaacct tttggcctgg attacgaact
    65521 ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa gtgaccgtgc ggcagaatat
    65581 cagccagctg tttgacgacc ttgataatgg taacgccgac ctgctggcgg caggacttgt
    65641 ctataacagt gagcgggtaa aaaattatca gcctggccct acctattatt ccgtgtcaca
    65701 acaactggtt tataaagtgg gtcagtatcg cccacgtacg ctgggcaacc tgacggcgga
    65761 gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat ctccagaccc tgaaagaaac
    65821 aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa ggctctgcgg aattaatgga
    65881 agatgtcatc gaaggaaaac tcgattacac cattgctgat tctgtcgcca tcagcctgtt
    65941 tcagcgcgtt cacccggagc tcgccgtagc gctcgatatc accgatgaac aaccggtgac
    66001 ttggtttagc ccgttagatg gcgataatac cctttccgcc gccctgctcg acttcttcaa
    66061 cgaaatgaat gaagacggta cgctggcacg cattgaagag aaatacctgg ggcatggcga
    66121 tgattttgat tacgtcgata cgcgcacatt tttacgcgcc gtcgatgcgg tactgccgca
    66181 gttaaagccc ctgtttgaga aatacgccga agaaattgac tggcgtttgc tggccgctat
    66241 tgcttatcag gaatcgcact gggatgcaca ggccacttca ccgacgggtg tgcgcggcat
    66301 gatgatgtta accaaaaata ccgcgcaaag cctcggcatt acggatcgta ccgatgccga
    66361 acagagcatc agcggtggcg tgcgttattt gcaggatatg atgagtaaag tgccggaaag
    66421 tgtgccggag aacgagcgga tctggtttgc cctcgctgcg tacaatatgg gctatgcgca
    66481 tatgctggat gcccgcgccc tgacggcaaa aaccaaaggg aatcctgaca gttgggctga
    66541 cgtaaaacag cgtctgcctt tacttagcca gaaaccctat tacagcaagc tgacttacgg
    66601 ctacgctcgt ggacatgaag cctacgctta tgtcgaaaat attcgtaagt atcagattag
    66661 cctggtgggt tatctgcaag agaaagagaa gcaggctaca gaagcggcga tgcaactggc
    66721 gcaggattat ccggcggtat cgcctacgga gttgggcaaa gagaaatttc cttttctctc
    66781 gtttctttcc cagtcgtcat caaactattt gacccattct ccctctctgc tgttttccag
    66841 gaaagggagt gaagagaaac aaaattaatc cgtcgaggat tgcgcttttt tctgcgcttt
    66901 aatttcctgg cggcgcatgc gaaagaagtc actgagcaac gccgcgcact catccgccag
    66961 tattccttcc gtaatttcca ctcggtgatt catacccgga tgatgcagca catccattaa
    67021 agatcccgca gcgccagttt tcgcgtcacg cgcaccaaag accacgcgac caatgcgact
    67081 gtggatcatc gctccggcac acattacaca tggttcaagc gtgacataca acgtggcgtc
    67141 gatcagacga taattttgca tcaccagacc accctgccgc agggccatga tttctgcatg
    67201 tgcggtggga tcatggcgac caatcgggcg gttccagcct tcgccgatta cccgattgtt
    67261 atgcactaat accgcgccga ccggcacttc ccgctcatcc caggcacgtt tcgccagcgt
    67321 cagcgcgtga cgcatccagt attcgtggct aaattcgact tcagacaaaa agaaaactcc
    67381 ggttataaaa gcgcggcgca ttatacacgg acgctatgct ttactctatt ccagttgctg
    67441 gagttcaccg cgcggggtta ctcgccaacg atgctgacag aaataaagca gcggattgtc
    67501 ctgattactg tcgctatagc cactgtacag ccgcagcgga gtgccgattt tgcgctccag
    67561 ttgtgcgacc ttttcatgtc ccagacaacg catcgtcaat acccaaccac catagccacg
    67621 ctgaatttgg ctggcgataa gattaacccg cggcagccag ggcgtatcga aataaaccgc
    67681 ttcaaccagc ggctgcggag agccggtaat caaccagata tcagcatcgg aacttaacag
    67741 gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta acattgtcgc gaaaccagcg
    67801 cacgaaatcg gcctgcaacg tctgtaaacg tgcttcgctg tgaccaaaag tgcaccccca
    67861 cagaagcaga ctcatcggcc agcgtgccgc acgacctttt atcaataacg caatggctat
    67921 aatcggtaac aacggcagga caagtaacgc attcagcggt tggcgacgca gtaaatagcg
    67981 cagaaaactg ccgaacatat cctgctgatg caatgttcca tctaagtcaa aaaacaccac
    68041 acgacgctcg tgagttgcca aaccatactc ctctggactg aaacatcctg attaattttc
    68101 gctactacat agcctaacag atagatcatc acttttccgg caacaatcga ccgcatcatg
    68161 gctaactgga atttttaatt catggcaatt agcggcaatg gaatataaaa ttcactcgcg
    68221 tgtctctcat attgcctgtt gtcgccacta tttcatcgcg aggaaagata tgaacggctt
    68281 acttcgtatc cgtcagcgtt accaggggct tgcccagagt gataaaaaac tggcggatta
    68341 tctgctgcta caacctgata cggcgcgcca tttaagctct cagcaactgg ccaacgaagc
    68401 cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa ctcggctata aaggttttcc
    68461 ggcgcttaag ttggcgttga gtgaagcgct ggcaagccag ccggaatcgc cctccgtgcc
    68521 cattcataac caaatccgcg gtgatgatcc gttacggctg gtcggcgaaa aactgattaa
    68581 agaaaatact gccgctatgt acgcaacgct aaacgttaat agtgaagaga aactgcatga
    68641 atgcgtaaca atgttgcgct ctgcgcggcg gataattctg accggtattg gcgcttcggg
    68701 tctggtggcg caaaactttg cctggaagct gatgaagatt ggcttcaatg ctgccgcagt
    68761 gcgcgatatg catgcgctac tcgcaacagt acaggcgtcg tcccctgacg atctgttatt
    68821 agccatttcc tacaccggtg tacgacgcga gttaaacctg gcggcagatg agatgctgcg
    68881 agtgggcgga aaagtgctgg cgattaccgg ctttactccg aatgccctgc aacagcgtgc
    68941 ttctcattgc ctgtatacca ttgccgaaga acaagcgaca aacagcgctt caatctctgc
    69001 ttgtcacgct cagggaatgt taacggattt gctgttcatt gcgctgattc agcaggatct
    69061 ggaactggca ccagaacgta ttcgtcatag tgaagcgctg gtgaaaaaac tggtctgagt
    69121 aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt tgagagtttc cttatggcgt
    69181 tgttaatcac taaaaaatgc atcaattgtg atatgtgtga acccgaatgc ccgaatgagg
    69241 cgatttcaat gggagatcat atctacgaga ttaacagcga taagtgtacc gaatgcgtag
    69301 ggcactacga gacaccaacc tgccagaagg tgtgcccgat ccccaatact attgtgaaag
    69361 atccggcgca tgtcgagaca gaagaacagt tgtgggataa atttgtgctg atgcaccacg
    69421 cggataaaat ttaatattct actctggaag tagagtatta attatattac tgggaagcct
    69481 taacgccatt atatttattt aattgatgac attagcataa tcattcacta agttaattta
    69541 tatagtatct gccaagacac ttatttatag ttattaaagg cgcgtccgat tggttcaccg
    69601 gacgcacctt aagtacgttt ccttgtgtta taagaacaga aggatcagct gtaaaacagc
    69661 aatgatgatt ttgatgaccc gtttaatcag gtatcggcaa tcagtcattc gtttttcctt
    69721 aaacaaggaa tgcagccgtt cagattagcc cttacatctc cccaaaactg aacgtgcgag
    69781 ttattgaggg tgcatgctgc actccacacc agagctttga cgacaccact cgtttcaatg
    69841 ggggaattct gtggcatggt gtaaagcaca gcaaaatctt caataacgaa gccaatttta
    69901 atgtacttac gaattggcgg tcacgttaat ctttccatca atattgcttt cttcgtaaag
    69961 gctcgagttt ttatgctaaa gattgcaagt tgcttgtaaa agataagtac actgatccat
    70021 aatcgctgtt gttgagggtg catgctgcac aaaattaaag ttaaaaagta aaacccccgt
    70081 tccttaccag ttcgggggtt ttacttttta aagagaacgg tattattttt aactttcaat
    70141 aattaccgtg gcacaagcat agtgccgctc atctgccagc gttacatgca tatttgcaac
    70201 gcccagcttt tccgccagtt ttaatgcctc gccccatagc cgtagccgtg gtttgccgag
    70261 ctcatcattg aatacttcaa attgattaaa cgccagacca ttgcggatcc cggtgccaaa
    70321 cgcttttgct gcggcttctt tcacagcaaa acgcttcgcc agaaaacgca ccggctggtg
    70381 gtgcgttttc cagatagccc attcgttatc gcttaatacg cggcgtgcca ggcgatcacc
    70441 ggatcgggcg atcaccgctt cgatgcgagc gatctccaca atatccgtgc ctaaacctaa
    70501 tattgccatt agccacgcgc ttccagcatc agacgcttca tttctgccac cgcatctttc
    70561 agtccggtca tcactgcacg accaataatg gcatgaccga tattcagttc atgcatctca
    70621 gggatggcgg caatggcttt cacgttgtga taggtcagac cgtgtccggc gttaactttc
    70681 agaccgaggc ttgcggcaaa ggtcgcggct ttggcgatac gcgccagctc ttgcgcctgt
    70741 tcggcgtcag ttttggcatc agcatagcaa ccggtgtgga tctcgataaa cggtgcgcca
    70801 acctctgccg cagctttgat ctgctcttca tcggcgtcaa taaacagaga aacctgaatc
    70861 ccggcatctg ccagacgttt gcaggcatcg cgcattttgt cacgctgccc tgcgacatcc
    70921 aggccgcctt cggttgttac ttcctgacgc ttttccggta ccaggcagca aaaatgtggc
    70981 ttcgtctcaa cggcgatcgc cagcatctct tcggtcaccg ccatctccag attcatgcgg
    71041 gtatccagcg tctgacgcag gatgcgcacg tcgcggtcag taatgtgacg gcgatcttca
    71101 cgtaaatgca cggtaatgcc gtccgctccc gcctgctcgg caataaacgc ggcctgcacc
    71161 ggatccgggt aagcggtacc gcgcgcgttg cgcagcgtag cgatatggtc aatgttgacg
    71221 cctaacagta attcagccat gacaatcctc atcattcata atgtgttttc accgttcgct
    71281 taggcataaa ttgccggaac agttccctgc tctttaaagg tttaccgcca agatacggct
    71341 taagcgccat gcgggtaaag cgtttcgcag cgcgcagtgt gtctgcgtca ggaaattccc
    71401 gtgcgtttaa cgcttttaac tgccttccgg tgaacgtttt attgtcgata acgacgcttg
    71461 cgataaaccc tttttcttcg cgataacgat acgtcatggt gtcatctacc ggctcgccgc
    71521 tacccgcaca atgggtaaaa ttgacgccat aacccagatg cccgagcagt gccagttcaa
    71581 agcggcgcag cgcgggttct ggcgtaccag tgacccctgc aagagactga atgcagtgca
    71641 agtaatcgaa aaaaagttca gagaagcgcg tctcgtattc cagtacgcgg gagagaagtt
    71701 cgttgatgta cagaccgctg taaagcgtga taccgcttaa tggcagcgcc agcgagacgg
    71761 cttcagcact gcgcagcgtt ttgacttcgc cacgcccgcc aaaacgtagc aagagagggg
    71821 tgaaaggctg taatgcacct ttcagggtag agcgtttaga gcgtgcgcct ttggcaacca
    71881 gacgcacgcg ccccgattcc tccgtgaaga cgtccagcat caggctggtt tcgctccacg
    71941 ggcgactatg caggacaaat gcgcgctgcc agccttccat cggagttact cttaaagatc
    72001 gtcaacgtaa ccgagactgc gcagtgcgcg ttcgtcgtcg gcccaaccgg atttcacttt
    72061 tacccacagt tcaaggtgaa caggcgcttc gaacatttcc tgcatgtctt tacgcgcttc
    72121 aatcccgatg gttttgatct tggccccttt gttgccaatg accatcttct tctgcccttc
    72181 acgctcaacg agaatcaaac cgttgatgtc ataaccaccg cgttcgttag agacgaaacg
    72241 ttcgatctcc acggtcacgg agtacggcag ttcagcgccg aggaaacgca tcagtttttc
    72301 gcggatgatt tcagacgcca taaaacgctg tgagcgatcg gtgatgtaat cttccgggaa
    72361 gtgatgagtc gcttcaggta gatgcttacg cacgattgcc gcaatagtgt caacattcag
    72421 cccggtttcg gcagagattg gcacgatatc gaggaagttc atctggcttg ccaggaactg
    72481 caggtgcggc agcagatcgg ctttctcctg cacgttgtcc actttgttca ccgcgaggat
    72541 taccggcgct ttgccttcgc gcagtttgtt gagcaccatt tcgtcgtccg gcgtccagcg
    72601 ggtgccttca acgacaaaaa tcaccagctc aacatcgcca atagagctgc tcgccgcttt
    72661 gttcatcagg cggttaatgg cgcgtttttc ttccatatgc aggcccggtg tatcgacgta
    72721 gatcgcctga tacgcgcctt cagtatggat ccccacaatg cggtgacgag ttgtctgcgc
    72781 cttgcgggaa gtgatggaga ttttctgccc cagcagcttg ttcaacaatg tggatttgcc
    72841 aacgttcgga cgtccgacga tggcaataaa tccgcagtaa cttttatcga tgctcattcc
    72901 agctccagtt ttttcaacgc ctgttcggcg gcagcctgct cagccttacg acggcttgaa
    72961 cctgtgccaa ccaccggttc actcaggccg ctgacctggc agtggatagt aaattcctga
    73021 tcgtgcgctt cgccacgtac ctggactacc agataagtcg gcagcggcag atggcgaccc
    73081 tgcaaatatt cttgcaagcg cgttttcgga tctttttgtt tatcgcctgg gctaatttcg
    73141 tccaaacgag tttgatacca gttgaggatt aatttctcga cggtttgaat atcactgtcg
    73201 aggaatacgc caccaattaa tgcttcgacg gtgtcggcga gaattgactc acgacgaaat
    73261 ccaccgcttt taagttcacc tggccctaaa cgtaagcact cgcctaactc aaattcgcgc
    73321 gccagttccg ccagcgtatt gccacggacc agcgtggcgc gcatccggct catatcgcct
    73381 tcatccacac gagggaaacg gtgataaagc gcattggcga taacgtagct cagaatagag
    73441 tcgcctaaaa attctaaacg ctcgttatgt ttactgctgg cactacgatg agttaatgcc
    73501 tgctgcaaca gttcctgatg attaaaagtg tagcccagct tccgttgaag ccgattaatt
    73561 acgatggggt tcatgcgata ccaataaata aatgcgtcaa caattcagca cacgaaacag
    73621 acctgatata catggttctg ctaactgctt cgctgcagtt tcagtttacc ggtatatggg
    73681 gaccaacgct gtttcgtgtg ccgtggcaac ctggaggtgc caaccttaaa cttcggggga
    73741 atattctata cacaacgacg ggggatgtcg ttagccacgg gagatttatc tcataaataa
    73801 ttcacgttgt cgccataacg gcgacaacgt gaacgaagat ggctattaat ggatgccgcc
    73861 aatgcgactt aagcgcagac cagtcggcca ttcgccttct tgcttatcga agctcatcca
    73921 gatagccgtt gcccgaccga ccagattcgc ttccggcaca aagccccagt aacggctgtc
    73981 cgcgctgttg tcgcggttgt cgcccatcat gaagtattgt cccggaggaa caatccaggt
    74041 tgccagttgt tgccctggct gctggtaata catccccacc tgatcctgcg caatcggcac
    74101 tgtcagaatg cggtgcgtca catcacccag tgtctcttta cgctcggaaa gacgaattcc
    74161 attttctttg gtttcgtttt tcggcacttc aaagaatccg ctggtcgctt ccccaccatt
    74221 acggcgtgag aaggtctgaa cgaaatcgct cggttccacg tttgagtagg tgaccggcag
    74281 cgcgttttca cacgcctggc cggaactgca tcccggttga atcgtcagct cttttgagac
    74341 cggatcgtaa gtgactttat cgcccggtaa acccaccgcg cgcttgatgt aatcaagctt
    74401 tggatcttcc ggatatttaa agaccacgat atcgccgcgt ttcggatgac cggtttcgat
    74461 cagcgttttc tggtagatag gatctttaat gccataagca aacttctcta ccagaataaa
    74521 atcaccaatt aacagagtcg gcatcatcga acctgacggg atctggaacg gttcataaat
    74581 aaacgaacgc acaatcaata cgatagccag taccggaaaa acagaagcac cggtttccag
    74641 ccagccaggc ttcggcgcaa cctttttcaa cgttgcttta tccagtgagt caccggcagc
    74701 cgcctgcgcc gctgcctgac gttcccgccg tttaggtgcg aaaaagaatt tatccacgca
    74761 ccataaaatg cccgtcacca gtgtggcaat caccagaatc agggcaaaca tattcgccat
    74821 gccaactcct aagggttatt tgttgtcttt gccgacgtgc agaatggcga ggaacgcttc
    74881 ctgcggcagc tcgacgttac cgatctgctt catgcgtttc ttaccttctt tctgcttctg
    74941 cagcagcttt ttcttacggc tgatatcgcc gccataacat ttagccagta cgtttttacg
    75001 cagctgtttc acggtggatc gcgcaataat gtgcgtacca atcgctgcct gaatggcgat
    75061 atcaaactgc tggcgtggga tcagatcttt catcttctcc accaactcgc gaccgcggtt
    75121 ttgcgaatta tcacggtggg tgatcagcgc caacgcatca acacgttcac cgttgattaa
    75181 tacgtctaca cgtaccatgt cggacgcctg gaagcgtttg aagttgtaat ccagagacgc
    75241 ataaccacgc gaggtagatt tcaggcgatc gaagaagtcg agcaccactt ccgccatcgg
    75301 gatctcgtac gtcagcgcca cctgattacc gtggtaaacc atattggtct gcacgccgcg
    75361 tttttctacg cacagcgtaa taacgttgcc gagatatgcc tgcggcagca gcatgtgaca
    75421 ttcggcaatc ggctcgcgca gttcgtagat gttatttacc gctggcagct tggatggact
    75481 gtcgacgtaa ataacttctc ttgacgtggt ttcaacttca tacactacgg tcggcgcagt
    75541 ggtgatcaga tccagatcgt attcacgttc cagacgttcc tggatgatct ccatgtgcag
    75601 caggccgagg aagccgcagc ggaaaccaaa gcccagcgcg ctggagcttt ccggctcata
    75661 gaacagtgaa gcatcgttca ggctgagttt acccagcgcg tcacggaagg cttcatagtc
    75721 gtcggaactt accgggaaca gaccggcgta tacctgcggt ttgactttct taaagccagg
    75781 cagcgccttt tctgccggat tacgcgccag cgttaaggta tcgccgactg gagcgccgtg
    75841 gatatcttta atcgcacata ccagccagcc tacttcgcca catttcagtt cagtgcggtc
    75901 aacctgcttc ggcgtgaaga tgcccagacg gtcggcgtta taggtctgcc cggtactcat
    75961 gactttcact ttgtcgccct tacgcagggt gccgttttta atacggataa gtgaaacaac
    76021 gcccagatag ttgtcgaacc aggagtcgat aattagtgcc tgcaacgggc cttccggatc
    76081 gccttccggc ggcggaatgt cgcgcaccag acgttcgaga acgtcctgca cgccaacgcc
    76141 ggttttcgct gaacagcgca ccgcgtcggt ggcgtcgatg ccgacgatat cttcaatttc
    76201 ttccgccacg cgttcaggat cggctgccgg caggtcaatc ttgttcagta ccggcaccac
    76261 ttcgagatcc atttccatgg cggtgtagca gtttgccagg gtttgcgctt ctacgccctg
    76321 cccggcgtcg accaccagca atgcaccttc acaggcagcc agcgaacggg aaacttcata
    76381 ggagaagtct acgtggcccg gggtgtcgat aaagttaagc tgataggttt cgccgtcaga
    76441 cgctttgtag tccagcgtca cgctttgcgc tttgatggta atgccacgct cacgctcaag
    76501 atccatggaa tcgagaacct gcgcctccat ttcacggtca gacaggccac cgcagatctg
    76561 gataatacgg tcagacagcg tcgatttacc gtggtcaatg tgagctatga tcgaaaagtt
    76621 acgtatattc ttcataaagt atgattattg tgccttaatg cccggttaac caggctttta
    76681 gaagtcgctg ttctgagctt aacgtctgta ttaatagaaa cgccgcattc tacactacaa
    76741 cattgaggcg aggaaatgtt cataccgtat ggattgtggt atctggaaac gtcctcgcat
    76801 ttgttatgca aaatgcaata aagccagtga aatcactggc tcgcgtcttc cgaagatgtt
    76861 tcaaatcgca caaggccagg cggcaatgcc acgcttaaga tgatcggttg ccattctgcc
    76921 cgggcagcaa acttacgcga gtagccgcgc gcaatcagga acccgccaat accaccgagg
    76981 atcgcaccac ataatgctgc gacgtcagaa gcaaagagta gctgaaatag cgaagcgatg
    77041 aggaataatc ccaccagcgg cgacatataa accagtaatg cggagctaag caggctgcct
    77101 tcggcgatcc ccaattccac tttttgcccc ggcactaacg gctcatcaca gggtacgaca
    77161 atggtatgcg tggtttgcgg gccaagttta tttaacacgc ggctaccgca accggcgcgt
    77221 gaagcgcagc tgctgcatga agctttaaca tcacaactga ccagcgcctg cccgttttgc
    77281 caggagacga cggtagccca ctctttgatc attgcgctgc cccgaactta atattctcgg
    77341 caatgcgttt cgccgtttgc ggcggcagtt caccgacaat ggtgatttcg gcgttatcac
    77401 gtacgcttgt actgacggtt ctgcgtccgg tgcgcaacat ctgatcggtg ctcgatggcg
    77461 tagcgcggtt aacgtttacc gagaagctga ataatccgtc ggaatagaga cgtgattcga
    77521 taggcatgtt gtccatcgtc ggtagcggac gtcgactact ggaaacttcg ctaaaaccct
    77581 gtggcaacca ggttggcgtc cagctgaatt tagctttttc acctacagga acagaaagca
    77641 acggcggcaa atttgccttc gccagcgtct gcatactgct gctgatatcc tgattgacgt
    77701 taaaagcaat cacgcgaaat tgttccagcg tttcaccatc gcgatcaagg agatcaaccc
    77761 gcatcggtaa tttcgattcg gtgtccatcc acacgatgta gctgtagcgt gtaccatctc
    77821 gggcaacaac gcgaatgact tcgcaaagac gatcagcaat acgcgtgcgc ccgacggaga
    77881 taaagtcgta gtaaggagaa aggcgtttga aatcggtata gataagcgat ggcagagaat
    77941 caacaatgta atcgccatta agcgtgaacg gttcaagtcc cggttcaaaa tagctgattt
    78001 cattgccgcg ctgtaccact tcccggcgcg ggccatccat ttgcaacaat tgtgcaagag
    78061 ggcggttatc gaggcgtgca tgtcgataac gcagagactc aacaccctgt ttattgatgc
    78121 tgatgaatga cagctcgtaa ttcagtgact gactggccag gttcatctgc tgtaataacg
    78181 ccccggacgc gggagtggcc gaggcgttag cagagaataa caggctacct gtcactaatg
    78241 acatggcaaa ccaaagttgc ttcattactg cgattgcgtt cctaaagttt gaattcctgg
    78301 cacctgtaca gcggcttgct gggtttgcgc ctgctcaaac tgaagctgtt cagagtggag
    78361 tcggcgttgc agttcgtaat cctgcaacat tgcattaatg cgacgacgct gctcctgtac
    78421 ctgctgctgt tgaccattgt ttgcggtcgc ttcagaaggt actcccaggc ttaccgggct
    78481 ggctttaccc atcatcggca gtgtattaaa taccggcgtt tcgggctgct gggacgtttc
    78541 agattgtcca ttatagtgct ggacgccaac gataactgca agcgatacgc atgctgctac
    78601 gcccatttgg gtaagctgtg ccgcccacgg acgtactttc tgccagaatg gcattttctg
    78661 ccattgatgc ggcgcaggct gggcttccgg gatcaatgtc gccggttgac gtactggctc
    78721 ttcttcaatg gcggccatca cgcgtgaaga gatatcgaaa tggagcacct cgggagtatc
    78781 accccgcatt gagtcacgga ttaagtgata gctttcccag gttttctgca tttctgggtt
    78841 atgagccagt tcgttaagca gctcactatc cagcgtttcg ccatccatta aagcggaaag
    78901 ttgttctttc tgcatgccta atacccttat ccagtatccc gctatcgtca acgcctgata
    78961 agcggttgaa ctttgttatc aatagcttcc ctcgctcgga agatacgtga acgcaccgta
    79021 cctaccggac aatccatgat agcggctatc tcttcatagc tcaggccatc cagctcccgc
    79081 aaggttattg ccatgcgcaa atcttccggg agggactcaa tagttcggaa aactatctgt
    79141 ctcagttctt ctgacaacat taagttctca gggttcgaaa tttctttcaa cgcgccgcca
    79201 ctttcgaagt tttcagcttc aatggcatcc acatcactgg aaggtggacg acgcccctga
    79261 gcaaccaggt aatttttcgc tgtatttaca gcaatccgat acagccatgt ataaaaagcg
    79321 ctatctcccc ggaacgaatc cagcgcacga taggctttaa taaaagcttc ttgtaccaca
    79381 tcgggaacat cacccgacgg cacatagcgg gaaaccagac tcgccacttt atgctgatag
    79441 cgcactacca gtaggttaaa ggctttctga tctcccttct ggacccgttc aaccaggacc
    79501 tggtccgtta actgctcgct catccgaggt aaagtctccc caaaccaaat ttccacgcgc
    79561 tatcgaaacg ccactccatt agctgcaatt tgagcaagca aagggttaga gtgtctcgtt
    79621 tttgtaaagt tccgtaacgc atctgttttt gtttgtcatg ctgtagacgg atcattatct
    79681 atcattataa gtctacagaa tctgaacatc gcattatctg tgtagaaatg cccatttaac
    79741 tgcctgaaga gtaacccaac ggccttttta tttcaccacc taatcctcca ccagccagta
    79801 acttctcttt ttctcgccgc cctgcgtcag cgtgtttagc cactgtaaca aatattaaaa
    79861 tagcaggtgt ttattcgcac aacatgatgc tatgctgacc aaacggtgtt tagtaaatta
    79921 aacaaagaaa atgaatactc tcccagaaca ttcatgtgac gtgttgatta tcggtagcgg
    79981 cgcagccgga ctttcactgg cgctacgcct ggctgaccag catcaggtca tcgttctaag
    80041 taaaggcccg gtaacggaag gttcaacatt ttatgcccag ggcggtattg ccgccgtgtt
    80101 tgatgaaact gacagcattg actcgcatgt ggaagacaca ttgattgccg gggctggtat
    80161 ttgcgatcgc catgcagttg aatttgtcgc cagcaatgca cgatcctgtg tgcaatggtt
    80221 aattgaccag ggggttttgt ttgataccca cgttcaaccg aatggcgaag aaagctacca
    80281 tctgacccgt gaaggtggac atagtcaccg tcgtattctt catgccgccg acgccaccgg
    80341 tagagaagta caatccacgc tggtgagcaa ggcgcagaac catccgaata ttcgtgtgct
    80401 ggagcgcagc aacgcggttg atctgattgt ctctgacaaa attggcctgc cgggcacgcg
    80461 acgggttgtt ggcgcgtggg tatggaaccg aaataaagaa acggtggaaa cctgccacgc
    80521 aaaagcagtg gtactggcaa ccggcggtgc gtcgaaggtt tatcagtaca ccaccaatcc
    80581 ggatatttct tctggcgatg gcattgctat ggcgtggcgc gcaggctgtc gggttgccaa
    80641 tctcgaattt aatcagttcc accctaccgc actgtatcac ccacaggcac gcaatttcct
    80701 gttaacggaa gcactgcgcg gcgaaggcgc ttatctcaag cgcccggatg gcacgcgttt
    80761 tatgcccgat tttgatgagc gcggcgaact ggccccgcgc gatattgtcg cccgcgccat
    80821 tgaccatgaa atgaaacgcc tcggcgcaga ttgtatgttc ctcgacatca gccataagcc
    80881 cgccgatttt attcgccagc atttcccgat gatttatgaa aaattgctcg ggctggggat
    80941 tgatctcaca caagaaccgg taccgattgt gcctgctgca cattatacct gcggtggtgt
    81001 aatggttgat aatcatgggc gtacggacgt cgagggcttg tatgccattg gcgaggtgag
    81061 ttataccggc ttacacggcg ctaaccgcat ggcctcgaat tcattgctgg agtgtctggt
    81121 ctatggctgg tcggcggcag aagatatcac cagacgtatg cctgatgccc acggcgtcag
    81181 tacgttaccg ccgtgggatg aaagccgcgt tgagaaccct gacgaacggg tagtaattca
    81241 gcataactgg cacgagctac ggctgtttat gtgggattac gttggcattg tgcgcacaac
    81301 gaagcgcctg gaacgcgccc tgcggcggat aaccatgctc caacaagaaa tagacgaata
    81361 ttacgcccat ttccgcgtct caaataattt gctggagctg cgtaatctgg tacaggttgc
    81421 cgagttgatt gttcgctgtg cgatgatgcg caaggagagc cgtggactgc attttacgct
    81481 ggattacccg gaactgctca cccattccgg tccatcgatc ctttctcccg gcaatcatta
    81541 cataaacaga taaaaagcct gggtcagcgc tgtatacgct tcggaatagt tctggtctgg
    81601 cccacgaatg actaagcgat cactaaagca ttctcccgcc tgcggggaga atgccagcag
    81661 cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc gtccgcaaac gtaaatgcca
    81721 gcccatgctt aatgccagtt cagtaaaacc attaccaatc tgctctggca gcaccacgca
    81781 gaaaaatccc tcttcggtaa tgcactccgc cgcacaggtc agcagcgatg ggtgatcaag
    81841 cgtagtggta tagcgagcct gttcccgttg aggtgtcgca cactctactc cctgctgata
    81901 gtaaggtggg ttactgatga ttaaatcgaa gcgcgcggtc tgctgtgtta cccactgctg
    81961 aatatccgcc gtatggacgt taatccgctc tgcccacggg gactggttga tattttcctg
    82021 cgcctgcgcg gcagcttcac tttccagctc aacggcatca attatcacgc tgtcactggt
    82081 tcgctgcgcc agcatcagtg ccagcaaccc gctacccgcg ccgatatcaa ggcaacgttt
    82141 caccccagcc accggtgccc atgcgcccag caaaatgcca tccgttccca ctttcatcgc
    82201 acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat ccattacgac gaagcacgga
    82261 tgtagactgt gacatgaaaa taaaaccttg caggaaaaac ggcgatagca ccgggtgaga
    82321 acaatacccg agaagcgata tccatacaaa cagatgaaga ttgcagccgt aacgtctata
    82381 atcagcgccc cacacagagg tagaacatga ctgtaacgac tttttccgaa cttgaactcg
    82441 acgaaagcct gctggaagcc ctccaggata aaggtttcac tcgcccgacc gccattcagg
    82501 ctgccgccat tccgcctgcg ctcgatggcc gtgatgtact cggttctgcg ccgacaggca
    82561 ccggtaaaac ggcggcgtat ctgctgccag cgttgcagca cctgctcgat ttcccacgta
    82621 agaaatccgg tccaccgcgt attttgatcc tcaccccaac tcgcgagctg gcgatgcagg
    82681 tggccgatca tgcccgcgaa ctggcgaaac atacgcatct ggatatcgcc accatcaccg
    82741 gcggcgtagc ctatatgaac cacgcggaag tgttcagcga aaatcaggac atcgtggtcg
    82801 ccacgaccgg acgtctgctg caatacataa aagaagagaa cttcgattgc cgcgcggttg
    82861 aaacgctgat cctcgacgaa gcagaccgta tgctggatat gggcttcgct caggatatcg
    82921 aacatattgc tggcgaaacg cgctggcgta aacagaccct gctcttttcg gcaacgctgg
    82981 aaggcgatgc gattcaggac tttgccgagc gtctgctgga agatccggtg gaggtttctg
    83041 ccaatccgtc cacccgtgag cgcaaaaaaa ttcatcagtg gtattaccgc gccgatgacc
    83101 tggaacataa aaccgcattg ctggtgcatc tgttaaaaca gccggaagcg acccgctcaa
    83161 ttgtgtttgt gcgtaagcgt gaacgtgtgc atgagctggc aaactggctg cgcgaagcgg
    83221 gcatcaacaa ctgctatctc gaaggtgaga tggtacaagg caagcgtaac gaagcgatca
    83281 agcgtttgac cgaaggtcgc gtaaacgtac tggttgcaac cgatgttgcc gcgcgcggta
    83341 tcgacattcc tgacgtcagc cacgtcttta acttcgatat gccgcgcagt ggcgatactt
    83401 atttgcaccg tatcggacgt accgcgcgcg ccggtcgtaa aggcaccgca atttcgctgg
    83461 tggaagccca tgaccatttg ctgctgggca aggttggccg ttacattgaa gagccaatta
    83521 aagctcgcgt tattgatgag ttacgcccga aaacgcgtgc gccaagcgaa aagcagaccg
    83581 gcaagccatc gaagaaagta ctggctaaac gtgccgagaa gaaaaaagct aaagagaaag
    83641 agaagccgcg ggtgaaaaaa cgccatcgcg acaccaaaaa tattggtaag cgccgtaaac
    83701 caagcggaac gggcgtgcca ccgcaaacga cagaagagta atctcaatgc cgggtttaag
    83761 cctggtatta aaagtgctgg caaacgcaaa actgcctgat gcgctacgct tatcaggcct
    83821 acgtggctca tgcaatatat tgaatttgca cgatcttgta ggccggataa ggcgttcacg
    83881 ccgcatccgg catgaataaa gcgcactttg tcgacaagtt aaatgccggg tttaatcccg
    83941 acattatttt tatccagcca caaaactctc ttcaacacac tgaataaaag tgtgcatcgc
    84001 cgggctaacc gcttttccag catggtgtgc gcacatggcc gtaatggtct gcgactgttc
    84061 gccaaaaggc aattcaatta attctccgct ctcaagctct ttttccaccg caaagcgggg
    84121 caagtaactg acaccgatat tcgccgcgac acaacgtttg atactttcaa tgcttaacag
    84181 ctcaatggtg ttttccaccg tgatccgccg ctgacgcagc gtgctctcaa atatctgccg
    84241 gaagacacat tgcggttcgt tgataataaa gctacaggcg ttatgtcttc ccggctcagt
    84301 aaaatcgaca tctgcaattt gcggtgaagc caccagcacc agcgattgtt cacccaactc
    84361 tcgtcggttc agggcatcat cattccctac gcgataaaag acgccgacat cggcctcatc
    84421 attcagtagc gcatcacgga tcacgtaaca gttcagcgac tgcaacgata aacgcacttt
    84481 tggtgcgcgt tgccgaaaac gctgcaacac ctgcggcatt cggtaagaga gcaacgtttc
    84541 gcccgaaaca acgcgtagtt ccccgtccgg atctgactcc tttttagcag cctcacgaag
    84601 cgtatccatc actcgggtaa gttcataaat gtgcggcagc aacttttttc cttcgcgggt
    84661 aaggcacatc cgtcggccaa ttttctcaaa taactggact gaaaactcct gctcaagctg
    84721 ctgaatatga aaagtcaccg tcgattgtgt acagcacaat ttttgcgaag ctcgcaaaaa
    84781 ggaaccctct tccaccacgg ttttaagcgt aataaaacgg cgcagatcca taaccccaaa
    84841 cctatcgaaa atatcgaatc tagaatataa aaacattcat ttttttaaat gttccgtgtc
    84901 gggtactgtc taccaaaaca gaggagataa caagtgacac cgaccctttt aagtgctttt
    84961 tggacttaca ccctgattac cgctatgacg ccaggaccga acaatattct cgcccttagc
    85021 tctgctacgt cgcatggatt tcgtcaaagt acccgcgtgc tggcagggat gagtctggga
    85081 tttttgattg tgatgttact gtgtgcgggc atttcatttt cactggcagt gattgacccg
    85141 gcagcggtac accttttgag ttgggcgggg gcggcatata ttgtctggct ggcgtggaaa
    85201 atcgccacca gcccaacaaa ggaagacgga cttcaggcaa aaccaatcag cttttgggcc
    85261 agctttgctt tgcagtttgt gaacgtcaaa atcattttgt acggtgttac ggcactgtcg
    85321 acgtttgttc tgccgcaaac acaggcgcta agctgggtag ttggcgtcag cgttttgctg
    85381 gcgatgattg ggacgtttgg caatgtatgc tgggcgctgg cggggcatct gtttcagcaa
    85441 ttgtttcgcc agtatggtcg ccagttaaat atcgtgcttg ccctgttgct ggtctattgc
    85501 gcggtacgca ttttctatta acgaaaaaaa gcggaagagg tcgccctctt ccgcttagta
    85561 acttgctact taagccttac aggctttcag taaaggtacg agcgataacg tcgcgctgct
    85621 gttccggagt cagagagtta aagcgaactg cataaccgga tacacggatg gtcagctgcg
    85681 gatatttttc cggatgctta actgcatctt ccagagtttc acgacgcaga acgttaacgt
    85741 tcaggtgttg accaccttca acgcgaactt ctggtttcac ttctactgga acttcacggt
    85801 attcaatgtc acccagtttg cttactgcaa ccacttcatc ttctgcataa cctgcttttg
    85861 caacgatgca acgtgcttcg cctttttcgc tgtccagcag ccagaaagag ttcagcagat
    85921 cgtcgttagc ggctttagta atctggatac ctgtaatcat gtgatgcctc cccggcaaaa
    85981 ttatttgatt tgttcagcct gtcgcggcca attggtaaaa ccattgttgc ttgagtgtat
    86041 atatactcct caaccaccct tgattctttg atttaaatca ataaaaacca cacatcgatt
    86101 gtggtcgtaa atggatttta ttgttttaca tcaacttatg cgggtgtgaa attttaccaa
    86161 tttacatttt tttgcactcg tttaagtcta aaaaatgagc atgattttgt tctgtagaaa
    86221 gaagcagtta agctaggcgg attgaagatt cgcaggagag cgagatggct aacgaattaa
    86281 cctggcatga cgtgctggct gaagagaagc aacaacctta ttttcttaat acccttcaga
    86341 ccgtcgccag cgagcggcag tccggcgtca ctatctatcc accacaaaaa gatgtcttta
    86401 acgcgttccg ctttaccgag ttgggtgacg ttaaagtggt gattctcggc caggatcctt
    86461 atcacggacc gggacaggcg catggtctgg cattttccgt tcgtcccggc attgccattc
    86521 ctccgtcatt attgaatatg tataaagagc tggaaaatac tattccgggc ttcacccgcc
    86581 ctaatcatgg ctatctcgaa agctgggcgc gtcagggcgt tctgctgctc aatactgtgt
    86641 tgacggttcg cgcaggtcag gcgcattccc acgccagcct cggttgggag acgtttactg
    86701 ataaagtcat cagcctgatt aaccagcacc gcgaaggcgt ggtgtttttg ttgtggggat
    86761 cacatgcgca aaagaaaggg gcgattatag ataagcaacg ccatcatgta ctgaaagcac
    86821 cgcatccgtc gccgctttcg gcgcatcgtg gattctttgg ctgcaaccat tttgtgctgg
    86881 caaatcagtg gctggaacaa cgtggcgaga cgccgattga ctggatgcca gtattaccgg
    86941 cagagagtga gtaaatttgc ggtaaaatgc cggatggcag agttgccacc cggctgattt
    87001 atcaggcttt attctgacgc caccattcac caagcaaaac gccggttgca acagagatat
    87061 tcagcccggc aacgttgccc gtaccgtcaa tcttcacgcg cagatcgttc ggatcgcgtg
    87121 cggcatccgg taacccttca tattcctgac ccagcaccag taccattttc gctggcagac
    87181 tggttttgaa cagcggtttc ccctgctcgc tggaagtggt cactacggtg taacctgcct
    87241 gacggaaatc atccaacacg ttaacaatgt tgtcgccagt aatcggctga acgtgctctg
    87301 cgccgccttc tgcggtacgg atagccgccc ccgactccag cagtgccgca tcctgcacca
    87361 caacgccttt cacgccgaag tgcgcgcagc tgcgcatcat gccacccagg ttatgcgggt
    87421 tagattcgtt ttccagcgcc agtacacaat cctgcgcgcc tgcctggctt acccactgct
    87481 gcacggttgt gccattacgc tttttgatca ggaagcaaac gccgccgtga tgttccgtgc
    87541 ctgacgcttt tgtcaattcc gcttcatcca ccacatggta cgctttgcgg tttgctgcca
    87601 tccagcgcaa ggcttcttta aaacgcggcg ttacactctg gataaaccag gcgcgaacaa
    87661 tcgcttccgg acggctctgg aaaagtgcct gacatgcatt ttcgccgtag acgcgggttt
    87721 cttccgcacg ctgacgacgc aacacttccg gatcaataaa acttttacca ctgatgccac
    87781 cgtgatcggc cttttccggc gtctcatcac ccggcgcgcg ggaaaccgtg cgccacggcg
    87841 aatcttccca cttgcggtca cggggctgac tctgtttgtc atcgcgggcg gggcgacggc
    87901 caccgtcagc acgagatttt cctggacgcc cgcccccttt cccggtacgc gggttgtggg
    87961 tacgtttatc agaatcatca tcactgcgga catacatcac tttgaccttg ccgcttttac
    88021 ctttcatttc atcgttcatg cttttctcca ccagcgctgc gcgaagcgcg cagattaccc
    88081 gaagtccgcg cggttcgcca tgatttcgta ccaaagcctg cgactatcat acctattgaa
    88141 taaaacagat tgttgtctgg aacaatgtcc ccgataatat gtaacatatt agaaacatac
    88201 cggcgtcgtt gccgataagt ctccttactc atcccgaggt tagttatgaa taccgtttgt
    88261 acccattgtc aggccatcaa tcgcattccc gacgatcgga tcgaagatgc ggcaaaatgc
    88321 ggacgctgcg gtcacgactt gtttgacgga gaggtgatta atgcgaccgg tgaaacactc
    88381 gacaaattgc tgaaggatga tctacctgtg gtgatcgact tctgggcacc gtggtgcggc
    88441 ccctgccgta atttcgcacc aatttttgaa gatgtcgcgc aagagcgtag cggtaaagtg
    88501 cgctttgtga aagtgaatac cgaagctgaa cgtgaattga gcagtcgctt tggaattcgt
    88561 agtataccga cgatcatgat tttcaaaaac ggtcaggttg tcgacatgct taatggcgca
    88621 gtaccgaaag cgccgttcga tagctggctg aacgaatctc tttaatctta ccggggcgca
    88681 tcttgtgccc cgttttctcc tctgcgacaa tggcgttttt tcgacgctct cttatgaccg
    88741 aaaacgctgt tctccagtta cgcgccgagc gtattgcgcg cgcaacacgt ccttttcttg
    88801 cccgcggtaa tcgcgttcgt cgctgccaac gctgtctttt gccagagaaa ttatgtctct
    88861 gttcgacaat taccccagca caagcaaaaa gtcgcttctg tttgctgatg ttcgacaccg
    88921 agccaatgaa gcccagtaat accgggcgtc tgattgctga tattttgcct gataccgttg
    88981 cgtttcaatg gtcgcgtacc gaaccctcgc aagatttact ggatctggta caaaacccgg
    89041 actatcagcc aatggtggtc tttcccgctt cgtatgctga tgagcaacgg gaagtgatct
    89101 tcacaccacc tgccggtaag ccaccgctgt ttatcatgct cgatggtacc tggccggaag
    89161 ctcgcaagat gtttcgtaaa agtccgtatc tggataatct tcccgtcatt tccgtcgatc
    89221 tttcccggct ttctgcctat cgcctgcgtg aagcccaggc tgaaggccaa tattgtactg
    89281 ccgaggtagc catcgcactg ttagatatgg ccggcgatac cggggcggca gcagggttag
    89341 gcgagcattt tacccgcttt aaaacacgct atctggcagg aaaaacgcaa catctgggta
    89401 gcatcacagc agaacagtta gaaagcgttt aaaatcattc ggtcacttct gcgggagacc
    89461 ggtatgagtc agcgaggact ggaagcacta ctgcgaccaa aatcgatagc ggtaattggc
    89521 gcgtcgatga aacccaatcg cgcaggttac ctgatgatgc gtaacctgct ggcgggaggc
    89581 tttaacggac cggtactccc ggtgacgcca gcctggaaag cggtgttggg tgtgttggcc
    89641 tggccggata ttgccagctt gccctttaca cccgaccttg cggttttatg taccaatgcc
    89701 agccgtaatc ttgctcttct ggaagagctc ggcgagaaag gctgtaaaac ctgcattatt
    89761 ctttccgccc cggcatcgca acacgaagat ctccgcgcct gcgccctgcg ccataacatg
    89821 cgcctgcttg gaccaaacag tctgggttta ctggctccct ggcaaggtct gaatgccagc
    89881 ttttcgcctg tgccgattaa acgcggcaag ctggcgttta tttcacaatc ggcagccgtc
    89941 tccaacacca tcctcgactg ggcgcaacag cgtgagatgg gcttttccta ctttattgcg
    90001 ctcggcgaca gcctggatat cgacgttgat gaattgctcg actatctggc gcgcgacagt
    90061 aaaaccagcg ccatcctgct ctatctcgaa cagttaagcg acgcgcgacg ctttgtttcg
    90121 gcagcccgta gtgcctcgcg taataaaccg attctggtga ttaaaagcgg acgtagcccg
    90181 gcggcacagc gactgctcaa cacgacggca ggaatggacc cggcatggga tgcggctatt
    90241 cagcgtgccg gtttgttgcg ggtacaggat acccacgaac tgttttcggc ggtggaaacc
    90301 cttagccata tgcgcccgct gcgtggcgac cggttgatga ttatcagcaa cggtgctgcg
    90361 cctgccgcgc tggcgctgga tgccttatgg tcacgcaatg gcaagctggc aacgctaagc
    90421 gaagaaacct gccagaaact gcgcgatgca ctgccaggac atgtggcaat ctctaacccg
    90481 ctcgatctgc gcgatgacgc cagcagtgag cactatatta aaacgctgga cattctgctc
    90541 cacagccagg attttgatgc gctgatggtt attcattcgc ccagcgccgc tgctcccgca
    90601 acagaaagcg cgcaagtatt aattgaagcg gtaaagcatc atccccgcag caagtatgtt
    90661 tctctgctga cgaactggtg tggcgaacat tcctcgcaag aggcacgacg attattcagc
    90721 gaagccgggc tgccgaccta ccgtaccccg gaaggaacca tcactgcttt tatgcatatg
    90781 gtggagtacc ggcgtaatca gaagcaacta cgcgaaacgc cggcgttgcc cagcaatctg
    90841 acctccaata ccgcagaagc gcatcttctg ttgcaacagg cgattgccga aggagcaacg
    90901 tcactcgata cccatgaagt tcagcctatc ctgcaagcat atggcatgaa cacgctcccc
    90961 acctggattg ccagcgatag caccgaagcg gtgcatattg ccgaacagat tggttatccg
    91021 gtggcgctga aattgcgttc gccggatatt ccacataaat cggaagttca gggcgtcatg
    91081 ctctacctgc gtacagccaa tgaagtccag caagcggcaa acgccatttt cgatcgcgta
    91141 aaaatggcct ggccgcaggc acgggtccac ggcctgttgg tgcaaagtat ggctaaccgt
    91201 gctggtgctc aggagttgcg ggttgtggtt gagcacgatc cggttttcgg gccgttgatc
    91261 atgctgggcg aaggcggtgt ggagtggcgt cctgaagatc aagccgtcgt cgcgttaccg
    91321 ccgctgaaca tgaacctggc ccgctatctg gttattcagg ggatcaaaag taaaaagatt
    91381 cgtgcacgca gtgcgctacg cccattggat gttgcaggct tgagccagct tctggtgcag
    91441 gtttccaact tgattgtcga ttgcccggaa attcagcgtc tggatattca tcctttgctg
    91501 gcttctggca gtgaatttac cgcgctggat gtcacgctgg atatctcgcc gtttgaaggc
    91561 gataacgaga gtcggctggc agtgcgccct tatccgcatc agctggaaga atgggtagaa
    91621 ttgaaaaacg gtgaacgctg cttgttccgc ccgattttgc cagaagatga gccacaactt
    91681 cagcaattca tttcgcgagt caccaaagaa gatctttatt accgctactt tagcgagatc
    91741 aacgaattta cccatgaaga tttagccaac atgacacaga tcgactacga tcgggaaatg
    91801 gcgtttgtag cggtacgacg tattgatcaa acggaagaga tcctcggcgt cacgcgtgcg
    91861 atttccgatc ctgataacat cgatgccgaa tttgctgtgc tggttcgctc ggatctcaaa
    91921 gggttaggct taggtcgacg cttaatggaa aagttgatta cctatacgcg agatcacgga
    91981 ctacaacgtc tgaatggtat tacgatgcca aacaatcgtg gcatggtggc gctagcccgc
    92041 aagctcgggt ttaacgttga tatccagctc gaagagggga tcgttgggct tacgctaaat
    92101 cttgcccaac gcgaggaatc atgagtaagg tactggaaat gttgaccact taatcgggac
    92161 tggtgttatt attgcccgct tatgtcgtct gcattgcaca gaggaccctt caatgaacag
    92221 agaagaaatg cactgtgatg ttgtcaaaat ttaagcgtaa taaacatcaa caacaccttg
    92281 cccaactacc caagatttct caatcagttg atgatgtcga tttcttttac gctcccgccg
    92341 acttccggga gacgctgctg gaaaaaatag ccagcgcgaa gcagcgcatt tgcattgtcg
    92401 ccctgtatct cgaacaggat gacggtggca aaggcattct gaacgcgttg tatgaagcta
    92461 aaaggcagcg tccggaactg gatgtgcggg tgctggtcga ctggcatcgt gcacaacgtg
    92521 gacgcattgg cgctgcggca tctaacacta acgctgactg gtactgccgc atggcgcagg
    92581 aaaatccggg cgtagatgtt ccggtttatg gcgttccaat caatacccgt gaagcccttg
    92641 gtgttctgca ctttaaaggc tttatcatcg acgatagcgt actttatagc ggtgccagcc
    92701 tgaacgatgt ttacctgcat cagcacgata aatatcgcta cgaccgttat catcttatcc
    92761 gtaaccgtaa gatgtcagac attatgtttg aatgggttac acagaatatt atgaatggcc
    92821 gcggcgttaa tcgtctggat gatgttaatc ggccaaaaag cccggaaatc aagaacgata
    92881 ttcgtctgtt ccgccaggag ctgcgtgatg ccgcttatca tttccagggc gatgccgaca
    92941 acgatcagct ttccgtaacg ccgctagtgg ggctggggaa atcgagtctg ttgaacaaga
    93001 ccattttcca tcttatgcct tgtgccgagc agaaactaac catctgtacg ccatacttca
    93061 acctgccagc aatccttgtg cgcaatatta tccagttgct gcgcgaaggg aaaaaggtcg
    93121 aaattattgt tggtgataaa accgcgaacg acttctacat tccggaagat gaacctttca
    93181 agataattgg cgcattgcct tatctctatg agatcaatct gcgtcgtttc ctgagccgtt
    93241 tgcagtatta cgtcaatact gaccagctag tggttcggtt atggaaagat gacgacaaca
    93301 cctatcacct aaaagggatg tgggttgatg ataagtggat gttgatcacc ggtaataacc
    93361 tgaacccgcg cgcctggcgt ctggatctgg aaaacgccat tttgatccac gatccacaac
    93421 tggagcttgc gccgcagcga gagaaagaac tggagctgat ccgcgagcat accaccatcg
    93481 ttaagcacta tcgcgatctg caaagtattg ccgattatcc ggtgaaggtt cgtaaactca
    93541 tccgccgttt gcgccgtatc cgcatcgacc gattaattag ccgcatcctg taatcacaac
    93601 cccgtcctgt acggggtttg ttttttggag gccacgtttt gcgtattctt tttgtctgtt
    93661 cgctattgtt actttctgga tgcagccata tggctaacga tagctggagc gggcaggata
    93721 aagctcaaca ctttatcgcc tcggcgatgc tttccgccgc cggaaatgaa tattcacagc
    93781 atcaggggat gagccgggat cgcagtgcca tgtttggatt gatgttctct gtcagtttgg
    93841 gggcgtcaaa agagctttgg gatagccgcc ccgaagggag cggctggagc tggaaggatt
    93901 tggcctggga tgtcgccggt gcaagcaccg gctataccgt ctggcaactg acccgtcact
    93961 aaagacgcat ccccttccct ttgcgatgta gcatcaaaga aaccagaaac gccaccacgg
    94021 ccatcaaggt cacataccag aagaaggctg tttccattcc tattgatttc agcgacaacg
    94081 ctacgtactc cgccgaacca ccaaatatag cattagcgac cgcatatgac aggccaacgc
    94141 ctaatgcgcg aacctgtgcc gggaacatct cagccttcag tattccactg attgatgtat
    94201 aaaaactcac tatcagcagg gcacacatca ccagaccaaa agcggcataa ggcgaggaaa
    94261 cgttttgcaa tgctgagaga ataggaacgg taaaaatggc tgccagcgaa ccgaaacata
    94321 acattgaggt acggcgacca atcttatccg acagcgcgcc aatgagtggt tgaataagca
    94381 tgaatacaaa caatgcggca gtcataatgc cactcgccac gttggcatgc attcccgcag
    94441 tatttaccag atacttctgc atataagtgg tgaaggtata gaaacaaagg gagcccgcag
    94501 cggtaaaacc gagaaccatg atgaatgcac ggcgattgcg ccataatcct ttcagagatc
    94561 cagcttcttt taaagcgcgc gtttcttgtt gcgaagtttc atctaattga cgacgtaacc
    94621 acaacgccac aacagctaac acagctccta acgcgaaagg aatacgccat ccccactctc
    94681 tgagtgcagc gtcttccatg gtgtgttgta aaaccacgac aaccagtagg gctagcagtt
    94741 gtccgccgat caacgtcaca tactgaaatg atgcgtaaaa acctttgcgc ccttcaacgg
    94801 caacttcact catataggtg gcgctggtgc catattctcc gccaacggat aatccctgaa
    94861 ataaacgagc gagaagcaat aatgccggag cccacgtacc tatagtttca taacctggga
    94921 ggcaggcgat aaccagcgat ccgaaacaca tcatacacac cgataacagc atcgattttt
    94981 tgcgaccatg tttatcggct atgcggccaa atagccaacc gcctattggg cgcatcagga
    95041 atcccgcagc aaaaacacct gctgtttgta gtagttgagt cgtcgtgttc ccggaaggga
    95101 agaaaatgtg ggcgaagtag agtgaacaga acgagtagac atagaaatcg aaccactcga
    95161 ccagattacc tgaagaggcc cccacaatcg cccaaatgcg gcgacgagta tcactacttg
    95221 tcagtttgct gtctgccgtt acagtacttt cagccatgcc attatgtctc ctgccgtaat
    95281 ccgatgcttt tgtcggtcgc ttttatttat ttcttttggt aaagaaaatg ttatacattt
    95341 gttgcgtatc attatgcaac cttaaccatg aatttagtta gcaggaaaat ggttattgag
    95401 gagcttaagg ataaatttct ggtaaggagg acacgtatgg aagtaggcaa gttggggaaa
    95461 ccgcatccgt tgctgaatct ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg
    95521 catttttttc tgcgccaatg caaaaaggcc atcctgacgg atggcctttc gcttaatttg
    95581 atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc
    95641 gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctaagg ccgccaggca
    95701 aattctgttt tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct
    95761 tctct
//
LOCUS       DAPHJE010000003        83284 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPHJE010000003 DAPHJE010000000
VERSION     DAPHJE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.003"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            10..2709
                     /locus_tag="RH222_000224"
     CDS             10..2709
                     /locus_tag="RH222_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDV3795297.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQ
                     QEAPLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DGSQRAGKVLICTQVLEQSVDCDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHSARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCIEQRQDPEMANVILVTDDESCGYSAMEGLTGKVG"
     gene            2807..4369
                     /gene="casA"
                     /locus_tag="RH222_000225"
     CDS             2807..4369
                     /gene="casA"
                     /locus_tag="RH222_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDV3795298.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEVEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKMWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRFVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVNLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4366..4902
                     /gene="casB"
                     /locus_tag="RH222_000226"
                     /pseudo
     CDS             4366..4902
                     /gene="casB"
                     /locus_tag="RH222_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            4914..5969
                     /gene="cas7e"
                     /locus_tag="RH222_000227"
     CDS             4914..5969
                     /gene="cas7e"
                     /locus_tag="RH222_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDV3795299.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWAMAEKGTEQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQRSMKDVLDFICA"
     gene            5980..6726
                     /gene="cas5e"
                     /locus_tag="RH222_000228"
     CDS             5980..6726
                     /gene="cas5e"
                     /locus_tag="RH222_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDV3795300.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            6708..7358
                     /gene="cas6e"
                     /locus_tag="RH222_000229"
     CDS             6708..7358
                     /gene="cas6e"
                     /locus_tag="RH222_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDV3795301.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQQLCFNLRANPTICKS
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7355..8278
                     /gene="cas1e"
                     /locus_tag="RH222_000230"
     CDS             7355..8278
                     /gene="cas1e"
                     /locus_tag="RH222_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDV3795302.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8275..8568
                     /gene="cas2"
                     /locus_tag="RH222_000231"
     CDS             8275..8568
                     /gene="cas2"
                     /locus_tag="RH222_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV3795303.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(8897..9934)
                     /gene="iap"
                     /locus_tag="RH222_000232"
     CDS             complement(8897..9934)
                     /gene="iap"
                     /locus_tag="RH222_000232"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV3795304.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10186..11094
                     /gene="cysD"
                     /locus_tag="RH222_000233"
     CDS             10186..11094
                     /gene="cysD"
                     /locus_tag="RH222_000233"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV3795305.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11096..12523
                     /gene="cysN"
                     /locus_tag="RH222_000234"
     CDS             11096..12523
                     /gene="cysN"
                     /locus_tag="RH222_000234"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV3795306.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFINRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12523..13128
                     /gene="cysC"
                     /locus_tag="RH222_000235"
     CDS             12523..13128
                     /gene="cysC"
                     /locus_tag="RH222_000235"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV3795307.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13178..13501
                     /locus_tag="RH222_000236"
     CDS             13178..13501
                     /locus_tag="RH222_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV3795308.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            13695..14006
                     /gene="ftsB"
                     /locus_tag="RH222_000237"
     CDS             13695..14006
                     /gene="ftsB"
                     /locus_tag="RH222_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV3795309.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14025..14735
                     /gene="ispD"
                     /locus_tag="RH222_000238"
     CDS             14025..14735
                     /gene="ispD"
                     /locus_tag="RH222_000238"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV3795310.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14735..15214
                     /gene="ispF"
                     /locus_tag="RH222_000239"
     CDS             14735..15214
                     /gene="ispF"
                     /locus_tag="RH222_000239"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV3795311.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15211..16260
                     /gene="truD"
                     /locus_tag="RH222_000240"
     CDS             15211..16260
                     /gene="truD"
                     /locus_tag="RH222_000240"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV3795312.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16241..17002
                     /gene="surE"
                     /locus_tag="RH222_000241"
     CDS             16241..17002
                     /gene="surE"
                     /locus_tag="RH222_000241"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV3795313.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            16996..17622
                     /gene="pcm"
                     /locus_tag="RH222_000242"
     CDS             16996..17622
                     /gene="pcm"
                     /locus_tag="RH222_000242"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV3795314.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            17762..18901
                     /gene="nlpD"
                     /locus_tag="RH222_000243"
     CDS             17762..18901
                     /gene="nlpD"
                     /locus_tag="RH222_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV3795315.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            18964..19956
                     /gene="rpoS"
                     /locus_tag="RH222_000244"
     CDS             18964..19956
                     /gene="rpoS"
                     /locus_tag="RH222_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV3795316.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20050..21414)
                     /locus_tag="RH222_000245"
     CDS             complement(20050..21414)
                     /locus_tag="RH222_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV3795317.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(21503..22279)
                     /locus_tag="RH222_000246"
     CDS             complement(21503..22279)
                     /locus_tag="RH222_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV3795318.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(22284..22922)
                     /locus_tag="RH222_000247"
     CDS             complement(22284..22922)
                     /locus_tag="RH222_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3795319.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(22919..24181)
                     /locus_tag="RH222_000248"
     CDS             complement(22919..24181)
                     /locus_tag="RH222_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDV3795320.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(24178..25086)
                     /locus_tag="RH222_000249"
     CDS             complement(24178..25086)
                     /locus_tag="RH222_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV3795321.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            25282..26049
                     /locus_tag="RH222_000250"
     CDS             25282..26049
                     /locus_tag="RH222_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3795322.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(26100..26756)
                     /gene="pphB"
                     /locus_tag="RH222_000251"
     CDS             complement(26100..26756)
                     /gene="pphB"
                     /locus_tag="RH222_000251"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV3795323.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(26862..29423)
                     /gene="mutS"
                     /locus_tag="RH222_000252"
     CDS             complement(26862..29423)
                     /gene="mutS"
                     /locus_tag="RH222_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV3795324.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            29499..29633
                     /locus_tag="RH222_000253"
     CDS             29499..29633
                     /locus_tag="RH222_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795325.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            29710..30063
                     /locus_tag="RH222_000254"
     CDS             29710..30063
                     /locus_tag="RH222_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV3795326.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(30100..32178)
                     /gene="flhA"
                     /locus_tag="RH222_000255"
     CDS             complement(30100..32178)
                     /gene="flhA"
                     /locus_tag="RH222_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV3795327.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32252..33262)
                     /gene="hypE"
                     /locus_tag="RH222_000256"
     CDS             complement(32252..33262)
                     /gene="hypE"
                     /locus_tag="RH222_000256"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV3795328.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(33259..34380)
                     /gene="hypD"
                     /locus_tag="RH222_000257"
     CDS             complement(33259..34380)
                     /gene="hypD"
                     /locus_tag="RH222_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV3795329.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTNAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(34380..34652)
                     /gene="hypC"
                     /locus_tag="RH222_000258"
     CDS             complement(34380..34652)
                     /gene="hypC"
                     /locus_tag="RH222_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV3795330.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(34643..35515)
                     /gene="hypB"
                     /locus_tag="RH222_000259"
     CDS             complement(34643..35515)
                     /gene="hypB"
                     /locus_tag="RH222_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV3795331.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIAEAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(35519..35869)
                     /gene="hypA"
                     /locus_tag="RH222_000260"
     CDS             complement(35519..35869)
                     /gene="hypA"
                     /locus_tag="RH222_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV3795332.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            36081..36542
                     /gene="hycA"
                     /locus_tag="RH222_000261"
     CDS             36081..36542
                     /gene="hycA"
                     /locus_tag="RH222_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV3795333.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            36667..37278
                     /gene="hycB"
                     /locus_tag="RH222_000262"
     CDS             36667..37278
                     /gene="hycB"
                     /locus_tag="RH222_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV3795334.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            37275..39101
                     /gene="hycC"
                     /locus_tag="RH222_000263"
     CDS             37275..39101
                     /gene="hycC"
                     /locus_tag="RH222_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV3795335.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQALAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            39104..40027
                     /gene="hycD"
                     /locus_tag="RH222_000264"
     CDS             39104..40027
                     /gene="hycD"
                     /locus_tag="RH222_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV3795336.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            40045..41754
                     /gene="hycE"
                     /locus_tag="RH222_000265"
     CDS             40045..41754
                     /gene="hycE"
                     /locus_tag="RH222_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV3795337.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            41764..42306
                     /gene="hycF"
                     /locus_tag="RH222_000266"
     CDS             41764..42306
                     /gene="hycF"
                     /locus_tag="RH222_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV3795338.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            42306..43073
                     /gene="hycG"
                     /locus_tag="RH222_000267"
     CDS             42306..43073
                     /gene="hycG"
                     /locus_tag="RH222_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV3795339.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            43070..43480
                     /gene="hycH"
                     /locus_tag="RH222_000268"
     CDS             43070..43480
                     /gene="hycH"
                     /locus_tag="RH222_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV3795340.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            43473..43943
                     /gene="hycI"
                     /locus_tag="RH222_000269"
     CDS             43473..43943
                     /gene="hycI"
                     /locus_tag="RH222_000269"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV3795341.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            43968..44729
                     /locus_tag="RH222_000270"
     CDS             43968..44729
                     /locus_tag="RH222_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795342.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            44903..45214
                     /locus_tag="RH222_000271"
     CDS             44903..45214
                     /locus_tag="RH222_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HDV3795343.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            45211..45630
                     /locus_tag="RH222_000272"
     CDS             45211..45630
                     /locus_tag="RH222_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3795344.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(45744..47168)
                     /gene="ascB"
                     /locus_tag="RH222_000273"
     CDS             complement(45744..47168)
                     /gene="ascB"
                     /locus_tag="RH222_000273"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV3795345.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(47177..48634)
                     /gene="ascF"
                     /locus_tag="RH222_000274"
     CDS             complement(47177..48634)
                     /gene="ascF"
                     /locus_tag="RH222_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV3795346.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            48894..49904
                     /gene="ascG"
                     /locus_tag="RH222_000275"
     CDS             48894..49904
                     /gene="ascG"
                     /locus_tag="RH222_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV3795347.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            50053..50580
                     /gene="hydN"
                     /locus_tag="RH222_000276"
     CDS             50053..50580
                     /gene="hydN"
                     /locus_tag="RH222_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV3795348.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            50733..52985
                     /gene="hypF"
                     /locus_tag="RH222_000277"
     CDS             50733..52985
                     /gene="hypF"
                     /locus_tag="RH222_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV3795349.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LADKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(53113..54246)
                     /gene="norW"
                     /locus_tag="RH222_000278"
     CDS             complement(53113..54246)
                     /gene="norW"
                     /locus_tag="RH222_000278"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV3795350.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(54243..55682)
                     /gene="norV"
                     /locus_tag="RH222_000279"
     CDS             complement(54243..55682)
                     /gene="norV"
                     /locus_tag="RH222_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV3795351.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            55869..57383
                     /gene="norR"
                     /locus_tag="RH222_000280"
     CDS             55869..57383
                     /gene="norR"
                     /locus_tag="RH222_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV3795352.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(57380..58345)
                     /gene="gutQ"
                     /locus_tag="RH222_000281"
     CDS             complement(57380..58345)
                     /gene="gutQ"
                     /locus_tag="RH222_000281"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV3795353.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(58338..59111)
                     /gene="srlR"
                     /locus_tag="RH222_000282"
                     /gene_synonym="gutR"
     CDS             complement(58338..59111)
                     /gene="srlR"
                     /locus_tag="RH222_000282"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV3795354.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(59178..59537)
                     /gene="gutM"
                     /locus_tag="RH222_000283"
     CDS             complement(59178..59537)
                     /gene="gutM"
                     /locus_tag="RH222_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV3795355.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(59642..60421)
                     /gene="srlD"
                     /locus_tag="RH222_000284"
     CDS             complement(59642..60421)
                     /gene="srlD"
                     /locus_tag="RH222_000284"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV3795356.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(60425..60796)
                     /gene="srlB"
                     /locus_tag="RH222_000285"
                     /gene_synonym="gutB"
     CDS             complement(60425..60796)
                     /gene="srlB"
                     /locus_tag="RH222_000285"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV3795357.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(60807..61766)
                     /gene="srlE"
                     /locus_tag="RH222_000286"
                     /gene_synonym="gutE"
     CDS             complement(60807..61766)
                     /gene="srlE"
                     /locus_tag="RH222_000286"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV3795358.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(61763..62326)
                     /gene="srlA"
                     /locus_tag="RH222_000287"
                     /gene_synonym="gutA"
     CDS             complement(61763..62326)
                     /gene="srlA"
                     /locus_tag="RH222_000287"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV3795359.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAVFEKKMGIQLEQKVHLAGATS"
     gene            62582..63667
                     /gene="mltB"
                     /locus_tag="RH222_000288"
     CDS             62582..63667
                     /gene="mltB"
                     /locus_tag="RH222_000288"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV3795360.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            63812..64309
                     /gene="pncC"
                     /locus_tag="RH222_000289"
     CDS             63812..64309
                     /gene="pncC"
                     /locus_tag="RH222_000289"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV3795361.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            64389..65450
                     /gene="recA"
                     /locus_tag="RH222_000290"
     CDS             64389..65450
                     /gene="recA"
                     /locus_tag="RH222_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV3795362.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            65693..66193
                     /gene="recX"
                     /locus_tag="RH222_000291"
     CDS             65693..66193
                     /gene="recX"
                     /locus_tag="RH222_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV3795363.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            66321..68951
                     /gene="alaS"
                     /locus_tag="RH222_000292"
     CDS             66321..68951
                     /gene="alaS"
                     /locus_tag="RH222_000292"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV3795364.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            69186..69371
                     /gene="csrA"
                     /locus_tag="RH222_000293"
     CDS             69186..69371
                     /gene="csrA"
                     /locus_tag="RH222_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV3795365.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            69687..69779
                     /locus_tag="RH222_000294"
     tRNA            69687..69779
                     /locus_tag="RH222_000294"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69721..69723,aa:Ser,seq:gct)
     gene            69783..69859
                     /locus_tag="RH222_000295"
     tRNA            69783..69859
                     /locus_tag="RH222_000295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69817..69819,aa:Arg,seq:acg)
     gene            70057..70133
                     /locus_tag="RH222_000296"
     tRNA            70057..70133
                     /locus_tag="RH222_000296"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70091..70093,aa:Arg,seq:acg)
     gene            70197..70273
                     /locus_tag="RH222_000297"
     tRNA            70197..70273
                     /locus_tag="RH222_000297"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70231..70233,aa:Arg,seq:acg)
     gene            70554..71120
                     /gene="yqaB"
                     /locus_tag="RH222_000298"
     CDS             70554..71120
                     /gene="yqaB"
                     /locus_tag="RH222_000298"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV3795366.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDLHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            71117..71545
                     /locus_tag="RH222_000299"
     CDS             71117..71545
                     /locus_tag="RH222_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3795367.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            71618..73174
                     /gene="gshA"
                     /locus_tag="RH222_000300"
     CDS             71618..73174
                     /gene="gshA"
                     /locus_tag="RH222_000300"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV3795368.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            73324..73839
                     /gene="luxS"
                     /locus_tag="RH222_000301"
     CDS             73324..73839
                     /gene="luxS"
                     /locus_tag="RH222_000301"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV3795369.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(73903..75441)
                     /gene="emrB"
                     /locus_tag="RH222_000302"
     CDS             complement(73903..75441)
                     /gene="emrB"
                     /locus_tag="RH222_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV3795370.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(75458..76630)
                     /gene="emrA"
                     /locus_tag="RH222_000303"
     CDS             complement(75458..76630)
                     /gene="emrA"
                     /locus_tag="RH222_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV3795371.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(76757..77287)
                     /gene="emrR"
                     /locus_tag="RH222_000304"
     CDS             complement(76757..77287)
                     /gene="emrR"
                     /locus_tag="RH222_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV3795372.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(77378..77713)
                     /gene="ygaH"
                     /locus_tag="RH222_000305"
     CDS             complement(77378..77713)
                     /gene="ygaH"
                     /locus_tag="RH222_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV3795373.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            complement(77703..78440)
                     /gene="ygaZ"
                     /locus_tag="RH222_000306"
     CDS             complement(77703..78440)
                     /gene="ygaZ"
                     /locus_tag="RH222_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV3795374.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(78564..79748)
                     /locus_tag="RH222_000307"
     CDS             complement(78564..79748)
                     /locus_tag="RH222_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3795375.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(80040..81032)
                     /gene="proX"
                     /locus_tag="RH222_000308"
     CDS             complement(80040..81032)
                     /gene="proX"
                     /locus_tag="RH222_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV3795376.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(81090..82154)
                     /gene="proW"
                     /locus_tag="RH222_000309"
     CDS             complement(81090..82154)
                     /gene="proW"
                     /locus_tag="RH222_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV3795377.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(82147..>83284)
                     /gene="proV"
                     /locus_tag="RH222_000310"
     CDS             complement(82147..>83284)
                     /gene="proV"
                     /locus_tag="RH222_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV3795378.1"
                     /translation="KYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSG
                     KSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVL
                     DNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAIN
                     PDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQN
                     GEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRS
                     ALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPL
                     SELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT        19749 a        21330 c        22619 g        19586 t
ORIGIN      
        1 ttttaaaata tgcgtaaata tcctttaagt ttactgaagg ataaaaatat tgtgactttc
       61 tttgatttct ggggaaaaac ccgacgcggc gagaaagaag ggggcgacga ctatcacctt
      121 ctttgctggc attcgctgga tgtggccgcg atgggctatt taatggttaa aagaaattgc
      181 ttcgggctgg ctgattattt tcgtcaatta gggatttctg acaaggaaca ggcggctcaa
      241 tttttcgcct ggttgctgtg ctggcacgat attggaaaat ttgcccgctc ttttcaacaa
      301 ctttacctgc cccctgaact caagattcag gaaggtgcca gaaaaaatta cgaaaaaatc
      361 tctcattcaa cgctgggcta ctggttgtgg aatcattatt taagtgaatg tcaggagtta
      421 cttccttcat cttcgctctc tccgcgcaaa cttaggcgag tgatagaaat gtggatgccc
      481 gtgacgacag ggcatcatgg tcggccccct gatcgtatgg atgaattgga taattttctg
      541 cctgaagaca aagctgcagc acgagatttt ctccttgcaa tcaaggcact gtttccgcga
      601 atagagatcc ccgcattctg ggatgatgac gagggcattg aacttataaa acacctttcc
      661 tggtatatct ctgcaaccgt cgtactcgca gactggacag gttcgtcaac gcgatttttt
      721 ccacgcgtcg cacaggcaat ggatattaaa cattactggc agaaggcttt agtgcaggct
      781 caaaacgcat taaccgtctt tcctccaaaa gcagaaaccg cacctttcac cggaattaat
      841 acactgtttc cttttattga gaatccgaca ccgttacagc aaaaggtgct ggatctggat
      901 atcagccagc caggaccgca gttatttatt ctggaagatg tgactggcgc aggtaaaaca
      961 gaagcggcgc ttatcctggc gcacaggttg atagctgcgg ggaaagctca ggggctgttt
     1021 tttggtctgc caacaatggc aacggccaat gccatgtacg atcggctggt caaaacctgg
     1081 cttgctttct attcgccaga atcccgcccc agcctggtgc tggcacatag tgcccgcaca
     1141 ttaatggacc gctttaatga atcactctgg tccggtgatt tagtcgggtc agaagaaccg
     1201 gatgaacaaa cgttcagtca gggatgtgcg gcctggtttg ccgacagtaa caagaaggcg
     1261 ttgctggctg aaattggcgt cggcacgctg gatcaggcga tgatggcggt gatgccgttc
     1321 aaacataata atctgcggct tctggggctg agtaacaaaa tcctgctggc tgatgagatc
     1381 cacgcctgtg acgcttacat gtcgtgcatt cttgaagggc tgatcgagcg gcaggcgcgt
     1441 ggcggaaaca gcgttatttt gctttctgct acgttatctc aacagcagcg cgacaaactc
     1501 gtcgccgcct ttgcgcgtgg tgcagagggc cagcaagaag ccccactcct gggaaaagat
     1561 gattacccct ggctgacgca tgtcacgaaa accgatgtac attcgcaccg ggtagcgacg
     1621 cgcaaagaag ttgagcgtag cgtgagcgtg ggttggcttc atagcgaaca agaatgtatt
     1681 gcgcgtatcg aatcagcggt aagtcagggg aaatgcatcg cctggatccg gaattctgtc
     1741 gatgacgcta ttaaggttta tcgtcagtta cttgcccgcg gcgtcattcc tgcttccagc
     1801 ctttcacttt ttcatagccg ctttgctttt agcgatcgcc agcgaattga aacggaaacg
     1861 ctggcacgct ttggtaaaga agacggttca cagcgtgccg gaaaagtcct catttgtact
     1921 caggtcttag agcagagcgt tgattgtgac ctggacgaaa tgatctccga cctggccccc
     1981 attgatttgc taatccagcg agcaggacga ttacagcggc atatccgcga tattaatggt
     2041 cagttaaagc gtgacggaaa agacgagcgt tccccccctg aattgctgat tctggctccc
     2101 gtctgggacg actctcctgg cgacgaatgg tttggcagtg ccatgcgtaa cagtgcattt
     2161 gtctatcccg atcatgggcg aatctggctg acgcagcgtg tgctgcgtga gcaaggtgct
     2221 attcagatgc cacactcagc ccggcttctt attgaatccg tctacggtga ggacgtggta
     2281 atgccggaag gatttgcccg cagcgagcag gagcaagtgg gcaaatatta ctgtgatcgc
     2341 gcaagggcta aaaagtttgt gctgaacttc aggcccggct atgccgccaa tatcaacgat
     2401 tacctcccgg aaaaactgtc gacacgtctg gctgaggagt ctgtttccct gtggctggct
     2461 acctgtattg acggtgtggt gaagccttat gccaccggtg ctcacgcatg ggaaatgagc
     2521 gttgtcagag tgcgccgaag ctggtggaaa aaacatcggg atgagttttc tttactggaa
     2581 ggggatgcgt tcaggcagtg gtgcattgaa cagcggcaag atccggagat ggcaaacgtg
     2641 attttagtca ccgatgacga aagttgcggg tattcggcta tggaaggatt gactggcaag
     2701 gttggttgag atgcctgcga cagggctctc tgaatagttg atacaccgaa aaggatggaa
     2761 tgatggacaa ccgacctggg atggtctttt ttacgagagg atgcgtatga actcgttttc
     2821 acttctgaca accccgtggt tgcctgttcg tttcaaagat ggaacgacag gcaaactggc
     2881 gcctgtcgat ctggcggatg aaaatgttgt cgatattgct gcgccacggg cagatctcca
     2941 gggagcggca tggcagtttt tgctggggct actacaaacc agctttgcgc caaaagatca
     3001 tcgtcgttgg gatgatatct gggaagacgg actggaagtt gaaaagctac gggaagcatt
     3061 gctgtcctta gaacacgctt tccagtttgg gccagattcc ccttcattta tgcaggattt
     3121 tgaggcgctc acgggagata aagttcaggt cgcttcacta ctgcctgaga ttcccggcgc
     3181 tcaaacaacg aagtttaata aagaccactt tatcaagcgt ggtgtaacgg aatacctgtg
     3241 cccacattgt tctgcgttag ctctgttctc cctacagtta aatgcgccgt caggtggtaa
     3301 aggctaccgc accggcttac gcggcggtgg gccgatgacc acgctgattg aattacagga
     3361 atatcagggc aatcaacaaa ctcccttgtg gcgcaaaatg tggctcaacg tgatgccgca
     3421 ggatgaagcc gatttaccgc tacccaaaaa atttgacgat ctggttttcc cctggcttgg
     3481 cccgacgcgc accagcgaac tggccggtgc ggtggtaacc gatgatcagg ttaataaact
     3541 ccaggcgtac tggggaatgc cgcggcgtat tcgtattgat tttaatacca cgacagtcgg
     3601 caactgcgat atttgcggcg agcagaacga cgcgctcctg agcctgatga cgaccaaaaa
     3661 ttacggtgcg aattatgcca tgtggcagca tcccttaacg ccttaccgtg taccacttaa
     3721 agagggtggc gagttttact ccgttaaacc acaaccgggc ggtttaatct ggcgtgactg
     3781 gttaggcctt atcgaaacgg gtaaatcaga aaacaatacg gaacttcccg cgctggtggt
     3841 gaaactcttt aatgccagca gtctgaaaca ggcaaaagtg ggcctgtggg gatttggtta
     3901 tgatttcgac aacatgaaag cgcgctgttg gtacgaacac catttccctc tattgctcaa
     3961 taaaaaagaa ggccagatac caaagctgcg gctggctgcg caaacggctt cacggattct
     4021 aagtctgtta cggagtgcat tgaaagaagc atggttctct gatccaaaag gtgcaagggg
     4081 tgattttagt tttgtggata tcgacttctg gaacaaaact cagcatcgct ttctgaggtt
     4141 cgtgcgccaa attgaagaag gtcaggatgc ggatgaatta ctcagcaaat ggaataagga
     4201 aatttggtta ttcgcgcgtc aggattttga cgagcgtgta ttcactaatc cttatgagcc
     4261 cgttaatttg gaacgcatca tgaccgcgcg caagaaatat tttacaacat cggcggagaa
     4321 gcaaagtgct aaagccgcca gggagaaaaa gcaggaggct gctgaatgag tattgtaaaa
     4381 gaggagcaca aagccacgct gcgcaaatgg cacgaggaat tgcaggagaa acgcggcgaa
     4441 cgtgccagtc tgcggcgtag tacgacggtt aatgatgttt gtctgacgga tggatttcgc
     4501 ctgtttctga agaacagaca aataaagtgg caggattaac ctgagtggcg tatcaccgca
     4561 ctagcgctga tagccgcagt tagcgcgaat gttaaagcca ttgatgaacg gcagcctttt
     4621 gccgcgcagc tggcggcggt aatgtcaaag gggcgtttta ctcgcctgtc ggcagtaaaa
     4681 acaccggatg aattactgcg ccagttacgg cgcgcagtca gattattaaa cggttccgtc
     4741 aatcttgact ctctggcaga gggggtcttc cgctggtgcc aggagagcga cgacctgctc
     4801 aatcatcacc gccgccagca gcggccaacc gaatttatcc gtatccgctg ggcgctggaa
     4861 tattaccagg ctggcgacgc cgataacgaa caaaatcaat aaaggactat ctcatgacaa
     4921 catttattca gcttcatttg ttaaccgctt accctgctgc caaccttaac cgtgatgata
     4981 ctggtgcgcc aaaaactgtg gtactgggtg gcgcaacgcg tctgcgcgtt tcctcacaaa
     5041 gtctgaaacg tgcgtggcgc acttctgcac tttttgaaca ggcactggcg ggccatattg
     5101 gtattcgtag tgggcgtatt gcgcgtgagg cggcaactat cctgattgag aaaggaatcg
     5161 aagagaaaaa agccatcgaa tgggcggcaa aaattgcgga ttatcttggg aaagctaaaa
     5221 acgacaaaaa accaaaagat ccgctcacta acgccgaaac tgaacaatta gttcatatca
     5281 gcccggcaga atttgacgcc gtaaaagcgc tggcccatca gcttgcagaa gaaaagcgcg
     5341 cgccaaaaga ggaagatctc gcgttgttac gcaaagaccg tatggcggta gatattgcca
     5401 tgtttggtcg tatgctggcg aataaacccg agtttaatgt tgaagccgcc tgccaggtag
     5461 cgcacgcatt tggtgtcagt gaaacgattg tcgaagatga ttttttcacc gccgttgatg
     5521 atttgcgcca ggcttctgaa gatgccggtg ccgggcatct tggtgaaacc ggattcggtt
     5581 ctgcactgtt ctacacctat atctgcattg ataaagatct gctggtcgaa aacctcggcg
     5641 gtgacgaagc gttagctaat cagaccatac gcgcctttac ggaagccgcg cttaaagtct
     5701 ccccaaccgg caaacagaac agctttgcca gccgtgccta cgcctcctgg gcgatggcag
     5761 aaaaaggtac cgaacaaccg cgttctctgg cggcggcttt ctatgaaccc attaatggca
     5821 cccggcagtt agaggtggcg gtgcagcgta ttacaacgct tcgcgaaaac atgaatacgg
     5881 tctacgaaca gaagaccgaa tgcgcaagtt ttgacgtgat gaacaaacag agaagcatga
     5941 aggacgtgct ggactttatc tgcgcgtaag gggcggctta tgagccaata tctgattttt
     6001 cagcttcatg ggccaatggc atcctggggc gtcgatgccc ccggcgaagt gcgtcatacc
     6061 catgaactgc cttcgcgctc agcattgctg gggctgctgg ctgccggggt agggattcgg
     6121 cgtgatgata ccgaacgctt aaacgcgttt aatcgccact attcactggt ggtttgcgcc
     6181 agccgtaacc cgcgctgggc acgggattat cacactatcc agatgccaaa agaggtgcgt
     6241 aaagcgcgtt atttcagccg tcgcgaagag ttgagcgacc ctgatcttct gagcgcgatt
     6301 atctcccggc gcgactacta caccgatgcc tggtggatgg tggcggtggc aacaacagcc
     6361 gatgcgcctt acagcctcga acagttgcag gacggtttac gccatccagt ttttccgctt
     6421 tatctggggc gaaaaagtca tccgctggca ttacctcttg cgccgttact gctcgaaggc
     6481 aacgcgtgtg atgccttatg taatgcatac caacagtatc aggatcattt ccataaactg
     6541 aaagtctcac tcccgaaact tcaggatgaa tgctggtggg aaggggaaca cgacggcctg
     6601 gtcgcgagca aaatattacg ccgtcgggat gttcctttaa atcgtcagca gtggttgttt
     6661 ggggaacgca ccatcaatca ggggccgtgg ctcagcaagg aggaaccatg tacctctcaa
     6721 gaataacctt acataccggc cagctttcgc ctgcgcagtt gctgcatctg gtggatcgcg
     6781 gtgaatatgt gatgcatcag tggctgtggg atcttttccc tggcggcaaa gaaaggcaat
     6841 ttctttatcg tcgggaagaa ctccagggcg cgtttcgctt ttttgtcctc tcgcaggaac
     6901 gcccggcgga aagtgatacg ttcaccatag aatgccggtc atttgcgccc gagctgcgta
     6961 ccggacaaca actttgtttc aacctgcggg cgaatccaac aatctgtaaa tcgggcaagc
     7021 gccacgatct gctgatggag gcgaaacggc aggtgagggg acaggcggaa ggaagtgatg
     7081 tctggttgca tcaacaacag gcggcgctgg actggctggc agcgcagggg gaaagaagtg
     7141 gttttacact actggatacc tcggttgatg cctaccgaca acagcaactc cggcgggaaa
     7201 actcccggca actgatccag ttcagtagtg tggattatac ggggatgctt acggtcaccg
     7261 atcccgggtt atttctgcaa cgtcttagcc agggatatgg caaaagccgg gcgttcggct
     7321 gtggtctgat gctgatcaaa cccggagcag aggcgtgacg tttgtaccac tgagtccgat
     7381 cccgttaaaa gatcgcacct ccatgatctt cctccagtac ggtcagatcg acgtactgga
     7441 cggcgctttc gtgctgatcg acaaaaccgg gatccgcacg cacattccgg tgggatcggt
     7501 cgcctgcatt atgctcgaac cgggaacgag agtttcccac gcggcggtgc atctagccgc
     7561 cacggtgggg acgctactgg tctgggtcgg tgaagcgggc gttcgcgttt actcctccgg
     7621 acaacccgga ggggcgcggg cagataaatt actctaccag gcaaagctgg ctttaacgga
     7681 agatctacgg ttgaaggtgg tgcgcaaaat gtatgaatta cgttttcgtg aaccaccgcc
     7741 agctcgccgt tcagtggagc agttacgtgg aattgaggga tctcgcgttc gccagacgta
     7801 tgcattactg gcgaaacaat atggtgtgaa atggaatggt cgcaaatacg atcctaaaga
     7861 ctgggaaaaa ggcgatgttg tgaatcgctg catcagtgct gctacatcat gtctgtacgg
     7921 tatatctgaa gcggcagtat tagccgcggg atatgcgccc gctattgggt ttatccatag
     7981 tggcaaaccg ctttcatttg tttatgacat agccgatatc attaaatttg attcggttgt
     8041 gccaaaggca tttgaaatag cagcgaggca acccgcagaa cctgataaag aagtcagatt
     8101 agcctgtcgc gatattttcc gtagcactaa gttaacgggc aaattaatac cgttaattga
     8161 ggaagtcctt gctgcaggtg aaattgaacc accacaaccc gcgccggata tgttaccgcc
     8221 tgccatccct gaacctgaaa cgctgggtga tagtggtcac cgggggcgcg gcggatgagc
     8281 atggtcgtgg ttgttacaga aaatgtccct ccgcgcttac gtggacggct cgcaatctgg
     8341 ctactggaag tgcgtgccgg tgtgtatgtt ggtgatacat caaaacgtat tcgggagatg
     8401 atctggcagc aaattaccca actggctggt tgcggaaatg tggtgatggc ctgggcgacc
     8461 aataccgagt cgggttttga atttcagacc tggggagaaa acagacgtat tccggtggat
     8521 ttggatgggt tacgtttggt ttcttttctt cctgttgata atcaataagt tagacgttct
     8581 ttaaaaataa ggaaatgttt taatttagtt ggtagattgt tgatgcggaa taaatttgtt
     8641 taaaaacagt tatgtatgct tagtgtgttc cccgcgccag cggggataaa ccgttgcccg
     8701 cgcaattccg ggagcatccg caattgtgtt ccccgcgcca gcggggataa accgttcagg
     8761 gtgtgattcc gcataccaag cagtaagagt tccccgcgcc agcggggata aaccgggcgc
     8821 actggatgcg atgatggata tcacttagaa ttccccgccc ctgcggtaga acacccagct
     8881 cccattttca aacccatcaa gacgccttcg ccaactcctt caccagaggt agcattatcc
     8941 gcataacgtc acggcagcga cgttctattc ttccaggaag tgccttatca atatgctgtt
     9001 gattatccag tcttacgtca tgccagctat ttcccgcagg gaatgcggct gtttttgcgc
     9061 gttgctgata accatcctta ttcccaagat tccagttagt cgcttccacc gaaagtacag
     9121 caatgcccgc tttgtcgaat atttctgcgt cattacaaca cccagtgcct ttcggataat
     9181 ttttattcaa acccggattg gtcgttgcgg ctattccgtg actgcgtgca attgccagcg
     9241 ccctgtcgcg cgttaatttc cttactgctt caggggtttt tacaccgctg ttgaaataca
     9301 atttatcgcc aacaattaag ttatcgagat taatcaccag cagcgtattt ttcttttcgg
     9361 tgtcactcat ccgcttgagt aaattctcag cgcctaattt cccttcctct tcgccgctgg
     9421 tcgccacaaa tcgaatacca tactcggtag gcgtattttt caggcgttct gccaattcca
     9481 gcatgacacc taaacctgcg gcgttatcat ccattccttg taacgtcagc ccgccgagat
     9541 tggcatcggc gtcagcatcg ctcagcgggg cgtaagtatc cagatgcgcc ataatgatga
     9601 tctgctgcgg cgctttgcct tcatgagcgg caatcaccgt acttcccgtc acgttatgcc
     9661 agctcttacg attatcgcgg gcggtataaa tataccgact attaaatgtc cgaatatcac
     9721 tgcgataacc catttgctga aactgttggc gaatataatc ggcagataac atttctgcag
     9781 gagttccggt catgcgtccc ggaaagaaag tagcaatatg tcgtgcctga gtattagcaa
     9841 aatcgccagg tttaggtgac gaggcgtgta cggggagaat aaagcatacg ccgagcgcca
     9901 gggcagcggt acggtggcgc aatgcggaaa acatagtgag tccttaaata ccatgcaaat
     9961 ttttttaccg ccatagtatg aaactgccgc tgcgctaaaa caatttcaaa tcttcctaaa
    10021 cgcccgaaat ccggtgcctt aagcactttt tgatattagc tttgccaaat cgttattccg
    10081 ttaaggaact actcattcta attggtaatt tcattcgttc tcttacgctc cctatagtcg
    10141 aaacatctga tggcaagaaa atagcggtat tgcaaaggaa cggttatgga tcaaatacga
    10201 cttactcacc tgcggcaact ggaggcggaa agcatccaca ttattcgcga ggtggcggca
    10261 gaattctcaa atccggtgat gctctactct atcggtaaag attccagcgt catgctgcat
    10321 ctggcgcgca aggcgtttta tccaggtacg ctgcctttcc cgttgctgca tgtcgatacc
    10381 ggctggaaat tccgcgagat gtatgagttc cgcgatcgta cggcgaaagc ctacggctgc
    10441 gagctgctgg tgcataaaaa cccggaaggc gtggcgatgg ggattaatcc attcgtgcac
    10501 ggcagcgcga aacataccga tattatgaaa actgaaggcc tgaaacaggc gctgaacaaa
    10561 tacggttttg atgccgcctt cggtggtgcg cgccgtgacg aagagaaatc ccgcgctaaa
    10621 gagcgaatct actctttccg tgaccgcttc catcgctggg atccgaaaaa tcagcgccca
    10681 gagctgtggc acaactacaa cgggcaaatt aacaaaggcg aaagcatccg cgtcttcccg
    10741 ctctctaact ggaccgagca ggatatctgg caatacatct ggctggaaaa tatcgacatt
    10801 gtgccgctgt atctcgctgc ggaacgtccg gttctggaac gcgacggtat gttgatgatg
    10861 attgatgaca accgtatcga cctgcaaccg ggcgaagtga ttaaaaaacg gatggtgcgt
    10921 ttccgtacgc tgggctgctg gccgctgacc ggtgcggtgg agtcaaatgc acaaacacta
    10981 ccggagatca tcgaagagat gctggtttct accaccagtg aacgtcaggg ccgcgtgatt
    11041 gaccgcgacc aggcggggtc tatggagctg aaaaaacgtc aggggtattt ttaagatgaa
    11101 caccgcactt gcacaacaaa tcgccaatga aggcggcgtc gaagcctgga tgattgcgca
    11161 acaacataaa agcctgctgc gttttctgac ctgtggtagc gtcgatgacg gcaaaagtac
    11221 cctgattggt cgcctgctgc acgatacccg ccaaatctac gaagatcagc tctcatcgct
    11281 gcataacgac agtaaacgtc acggcaccca gggcgaaaag ctggatctgg cactgctggt
    11341 ggacggcctg caagctgagc gcgaacaggg catcaccatt gacgtggctt accgctattt
    11401 ctctaccgag aagcgtaaat ttattatcgc cgacacccca gggcacgagc agtacacccg
    11461 caatatggcg actggcgcat cgacatgtga actggcgatc ttactgatcg atgcccgtaa
    11521 aggcgtgctc gatcaaaccc gtcgtcacag ttttatctcc acactgttgg ggatcaaaca
    11581 tctggtcgtg gcgatcaaca aaatggatct ggtggattac agtgaaaaga cgttcacccg
    11641 tattcgtgaa gattatctga cctttgccgg gcagctgccg ggtaatctgg atatccgctt
    11701 tgtgccgctc tctgcactgg aaggcgacaa cgtggcttcg caaagtgaaa gtatggcgtg
    11761 gtacagcggt ccaacactgc tcgaagtgct ggaaaccgtg gagatccagc gagtggtgga
    11821 tgctcagcca atgcgcttcc cggtgcagta cgttaatcgc ccgaatctcg attttcgtgg
    11881 ttacgccgga acgctggcat ccggtcgcgt agaagtcggg caacgagtca aagtgctgcc
    11941 ctctggtgtg gaatcaaacg tcgcccggat cgtgactttt gatggcgatc gcgaagaagc
    12001 ctttgccgga gaagcgatca ccctggtgct gacggatgag atcgacatca gccgtggcga
    12061 tctgctgctg gcggcagacg aagcgttacc agctgtgcag agcgcgtcgg tggatgtggt
    12121 atggatggcg gaacagccgc tttccccggg ccagagttat gacatcaaaa ttgccggtaa
    12181 gaagacgcgt gctcgtgttg atggcattcg ttatcaggtt gatattaata accttaccca
    12241 acgcgaagtt gaaaacctgc cgctgaacgg catcggcctg gtggatctca cttttgacga
    12301 gccactggtg ttagatcgtt atcagcaaaa tccggttacc ggtgggctga tttttatcaa
    12361 tcgccttagc aatgtgaccg tgggcgccgg tatggtgcac gagccagtta gccaggcaac
    12421 tgctgcgcca tctgaattta gtgcattcga attggaattg aatgccctgg ttcgccgcca
    12481 ctttccacac tggggcgcgc gcgatttgct gggggataaa taatggcgct gcatgacgaa
    12541 aacgtcgtct ggcatagcca tccggtcact gtgcaacaac gcgagctaca ccacggtcat
    12601 cgtggtgtag tgctgtggtt taccggcctc tccgggtccg gtaaatcaac ggtcgccggg
    12661 gcgctggagg aggcgttaca taaactcggc gtcagtacgt atctgctgga tggcgataat
    12721 gttcgccacg gattatgcag cgatctcggt tttagcgatg ccgatcgtaa agagaatatc
    12781 cgtcgcgtcg gtgaagtggc gaatttgatg gttgaagccg gactggtggt gctgaccgca
    12841 tttatctcgc cacaccgcgc cgaacgccag atggttcgcg aacgcgtagg agaagggcgc
    12901 tttattgaag tgtttgtcga tacgccgctg gcgatttgcg aagcccgcga tccaaaaggc
    12961 ttatataaga aagcgcgtgc cggtgaactg cgcaacttta cgggaataga ttccgtttac
    13021 gaagcgcctg aatcggcaga aattcatctc aatggtgaac aattagtaac aaatttggta
    13081 cagcaattat tagatctgtt gagacagaac gatattatca gatcctgaga caccaccggg
    13141 tttccatgcc cggttcgtca aagtaacagg atcaaatatg cgtaatagcc ataacattac
    13201 actaacaaat aacgacagcc ttactgagga tgaagaaacc acatggtcac tgcctggtgc
    13261 cgtggtcggt tttatctcct ggttatttgc gctggcgatg ccgatgttga tttatggctc
    13321 taacacgctg ttcttcttta tctacacctg gcctttcttt ctggcgctga tgcccgtcgc
    13381 ggtagtggtg gggattgcgc tgcattcatt gatggacgga aagctacgct acagtattgt
    13441 tttcaccctg gtgacagtag gcattatgtt tggcgcactg tttatgtggc tactgggcta
    13501 atttgtactt tcccgtctcc tctgttcata attcaaaccg taactaataa tgagattatg
    13561 ttctgcacgc cctgggtata cgtaacaatg gacaaatgtg gtacatttgc ccgcgttgtc
    13621 gcggtatccc caacagagga tgtagagtcg tcttcggatg catgggatga tgatgccgtt
    13681 tttcaggggg caggatgggt aaactaacgc tgctgttgct ggctattctg gtctggctac
    13741 agtattcgct gtggttcggt aagaacggta tacatgacta tacccgcgtc aatgatgatg
    13801 tggcggcaca gcaagctaca aacgcgaaac ttaaagcgcg aaacgatcaa ctttttgccg
    13861 aaattgacga tctcaatggc ggccaggagg cgctcgaaga gcgtgcgcgt aatgaactca
    13921 gcatgaccag gccgggcgaa actttttatc gtctggtgcc tgacgcgtcg aagcgcgcac
    13981 agtctgcggg gcaaaacaat cgataaatca gcccgggaat taacatggca accactcatt
    14041 tggatgtttg cgccgtggtt ccggcggccg gatttggccg tcgaatgcaa acggaatgtc
    14101 ctaagcaata tctctcaatc ggtaatcaaa ccattcttga acactcggtg catgcgctgc
    14161 tggcgcatcc ccgggtgaaa cgagtcgtca ttgccataag tcctggcgat agccgttttg
    14221 cacaacttcc tctggcgaat catccgcaaa tcaccgttgt agatggcggt gatgagcgtg
    14281 ccgattccgt gctggcaggt ctgaaagccg ctggcgacgc gcagtgggta ttggtgcatg
    14341 acgccgctcg tccttgtttg catcaggatg acctcgcgcg attgttggcg ttgagcgaaa
    14401 ccagccgcac gggggggatc ctcgccgcac cagtgcgcga tactatgaaa cgtgccgaac
    14461 cgggcaaaaa tgccattgct cataccgttg atcgcaacgg cttatggcac gcgctgacgc
    14521 cgcaattttt ccctcgtgag ctgttacatg actgtctgac gcgcgctcta aatgaaggcg
    14581 cgactattac cgacgaagcc tcggcgctgg aatattgcgg attccatcct cagttggtcg
    14641 aaggccgtgc ggataacatt aaagtcacgc gcccggaaga tttggcactg gccgagtttt
    14701 acctcacccg aaccatccat caggagaata cataatgcga attggacacg gttttgacgt
    14761 acatgccttt ggcggtgaag gcccaattat cattggtggc gtacgcattc cttacgaaaa
    14821 aggattgctg gcgcattctg atggcgacgt ggcgctccat gcgttgaccg atgcattgct
    14881 tggcgcggcg gcgctggggg atatcggcaa gctgttcccg gataccgatc cggcatttaa
    14941 aggtgccgat agccgcgagc tgctacgcga agcctggcgt cgtattcagg cgaagggtta
    15001 tacccttggc aacgtcgatg tcactatcat cgctcaggca ccgaagatgt tgccgcacat
    15061 tccacaaatg cgcgtgttta ttgccgaaga tctcggctgc catatggatg atgttaacgt
    15121 gaaagccact actacggaaa aactgggatt taccggacgt ggggaaggga ttgcctgtga
    15181 agcggtggcg ctactcatta aggcaacaaa atgattgagt ttgataatct cacttacctc
    15241 cacggtaaac cgcaaggcac cgggctgctg aaagccaatc cggaagactt tgtggtggtg
    15301 gaagatttgg gctttgagcc tgatggtgaa ggtgagcata ttctggttag aatcctcaaa
    15361 aacggctgca atacccgttt tgtggcggat gcactggcga aattcctgaa aattcatgcc
    15421 cgtgaagtca gcttcgctgg gcaaaaagac aaacatgctg ttacggaaca gtggttatgc
    15481 gctcgcgtgc cgggcaagga aatgcccgat ctgagcgcct ttcaactgga aggctgccag
    15541 gtgctggagt atgcgcggca caagcgcaag ctgcgtttag gggcgctgaa aggaaacgcc
    15601 tttaccctgg ttctgcgcga agtgagcaat cgcgatgacg ttgaacaacg tctgatcgat
    15661 atttgcgtaa aaggtgtacc gaactacttc ggtgcccaac gttttgggat tggcggtagc
    15721 aacttgcagg gggcgcagcg ctgggcgcaa accaatactc cggtgcgcga tcgcaataaa
    15781 cggagttttt ggttgtcggc agcccgcagt gcgttgttta atcagattgt tgctgagcgc
    15841 ctcaaaaaag cagacgttaa tcaagttgtt gacggcgatg cgctacaatt agccggacgt
    15901 ggtagctggt ttgtagcaac caccgaagaa ctggcggaat tacagcgtcg cgtcaacgat
    15961 aaagagttga tgataaccgc cgcattgcca ggcagtggcg aatggggaac tcagcgtgaa
    16021 gcgctggcat tcgaacaagc agctgtcgcc gcagaaactg aattacaagc tttactggtg
    16081 cgcgaaaaag ttgaagccgc gcgcagagcg atgctgctgt atccgcaaca attaagctgg
    16141 aattggtggg atgacgtcac cgtagagatc cgtttctggc ttccggcggg tagttttgca
    16201 accagcgttg tcagggaact tatcaacaca acaggtgatt atgcgcatat tgctgagtaa
    16261 tgatgacggg gtacatgcac ccggtataca aacgctggcg aaagccttgc gtgagtttgc
    16321 tgacgttcag gtggtcgccc ccgatcgtaa ccgcagcggc gcttcaaatt ctctgacact
    16381 ggaatcctcc ctgcgcacgt ttacctttga aaatggtgat attgctgtgc aaatgggaac
    16441 cccgaccgat tgcgtctatc ttggcgtgaa tgctctgatg cgtccgcgcc cggacattgt
    16501 tgtgtccgga attaacgccg ggccgaatct gggggatgat gttatttatt ccggtacggt
    16561 agccgccgcg atggaaggcc gtcatttagg ttttccggcg cttgccgtct cgcttgacgg
    16621 gcataaacat tacgacactg ccgcggcggt aacctgttca attttgcgcg cactgtgtaa
    16681 agagccgctg cgcaccgggc gtattcttaa tattaacgtt ccggatttac ccttggatca
    16741 aatcaaaggt attcgcgtga cgcgctgcgg tacacgacat ccggcagatc aggtgatccc
    16801 gcagcaagat ccgcgcggca atacgctgta ctggattggc ccgccgggcg gtaaatgtga
    16861 tgctggtccg gggaccgatt ttgctgcggt agatgagggc tatgtctcca tcacgccgct
    16921 gcatgtggat ttaactgcgc atagcgcgca agatgtggtt tcagactggt taaacagcgt
    16981 gggagttggc acgcaatggt aagcagacgc gtacaagcac ttctggatca attacgtgcg
    17041 caaggtattc aggatgagca ggtgctgaat gcacttgccg ccgtgccgcg tgaaaaattc
    17101 gttgatgaag cgtttgaaca aaaagcctgg gacaatatcg ctttgccgat aggtcagggg
    17161 cagacaattt cgcagccata tatggtggcg cgaatgaccg aattactcga gctgacgccg
    17221 cagtcgcggg tgctggaaat tggcaccggt tcgggatatc aaacggcaat cctggcgcat
    17281 cttgtccagc atgtttgctc ggttgaacgg attaaaggct tgcagtggca ggcacgtcgc
    17341 cgcctgaaaa atcttgattt acataatgtt tcaacccgtc atggcgatgg atggcaaggt
    17401 tggcaggcac gtgcgccgtt tgacgctatc attgttacgg cggcaccgcc ggaaattcca
    17461 actgcgctaa tgacgcagct ggacgaaggc gggattctcg tcttacccgt aggggaggag
    17521 caccagtatt tgaaacgggt gcgtcgtcgg ggaggcgaat ttattatcga taccgtggag
    17581 gccgtgcgct ttgtcccttt agtgaagggt gagctggctt aaaacgtgag gaaatacctg
    17641 gatttttcct ggttattttg ccgcaggtca gcgtatcgtg aacatctttt ccagtgttca
    17701 gtagggtgcc ttgcacggta attatgtcac tggttattaa ccaatttttc ctgggggata
    17761 aatgagcgcg ggaagcccaa aattcaccgt tcgccgcatt gcggctttgt cactggtttc
    17821 gctatggctg gcaggctgtt ctgacacttc aaatccaccg gcaccggtca gctccgttaa
    17881 tggcaatgcg cctgcaaata ctaattctgg tatgttgatt acgccgccgc cgaaaatggg
    17941 gacgacgtct acagcgcagc aaccgcaaat tcagccggtg cagcagccac aaattcaggc
    18001 tactcaacaa ccgcaaatcc agccagtgca gccagtagct cagcagccgg tacagatgga
    18061 aaacggacgc atcgtctata accgtcagta tgggaacatt ccgaaaggca gttatagcgg
    18121 cagtacctat accgtgaaaa aaggcgacac acttttctat atcgcctgga ttactggcaa
    18181 cgatttccgt gaccttgctc agcgcaacaa tattcaggca ccatacgcgc tgaacgttgg
    18241 tcagaccttg caggtgggta atgcttccgg tacgccaatc actggcggaa atgccattac
    18301 ccaggccgac gcagcagagc aaggagttgt gatcaagcct gcacaaaatt ccaccgttgc
    18361 tgttgcgtcg caaccgacaa ttacgtattc tgagtcttcg ggtgaacaga gtgctaacaa
    18421 aatgttgccg aacaacaagc caactgcgac cacggtcaca gcgcctgtaa cggtaccaac
    18481 agcaagcaca accgagccga ctgtcagcag tacatcaacc agtacgccta tctccacctg
    18541 gcgctggccg actgagggca aagtgatcga aacctttggc gcttctgagg ggggcaacaa
    18601 ggggattgat atcgcaggca gcaaaggaca ggcaattatc gcgaccgcag atggccgcgt
    18661 tgtttatgct ggtaacgcgc tgcgcggcta cggtaatctg attatcatca aacataatga
    18721 tgattacctg agtgcctacg cccataacga cacaatgctg gtccgggaac aacaagaagt
    18781 taaggcgggg caaaaaatag cgaccatggg tagcaccgga accagttcaa cacgcttgca
    18841 ttttgaaatt cgttacaagg ggaaatccgt aaacccgctg cgttatttgc cgcagcgata
    18901 aatcgacgga accaggcttt tgcttgaatg ttccgtcaag ggatcacggg taggagccac
    18961 cttatgagtc agaatacgct gaaagttcat gatttaaatg aagatgcgga atttgatgag
    19021 aacggagttg aggtttttga cgaaaaggcc ttagtagaag aggaacccag tgataacgat
    19081 ttggccgaag aggaactgtt atcgcaggga gccacacagc gtgtgttgga cgcgactcag
    19141 ctttaccttg gtgagattgg ttattcacca ctgttaacgg ccgaagaaga agtttatttt
    19201 gcgcgtcgcg cactgcgtgg agatgtcgcc tctcgccgcc ggatgatcga gagtaacttg
    19261 cgtctggtgg taaaaattgc ccgccgttat ggcaatcgtg gtctggcgtt gctggacctt
    19321 atcgaagagg gcaacctggg gctgatccgc gcggtagaga agtttgaccc ggaacgtggt
    19381 ttccgcttct caacatacgc aacctggtgg attcgccaga cgattgaacg ggcgattatg
    19441 aaccaaaccc gtactattcg tttgccgatt cacatcgtaa aggagctgaa cgtttacctg
    19501 cgaaccgcac gtgagttgtc ccataagctg gaccatgaac caagtgcgga agagatcgca
    19561 gagcaactgg ataagccagt tgatgacgtc agccgtatgc ttcgtcttaa cgagcgcatt
    19621 acctcggtag acaccccgct gggtggtgat tccgaaaaag cgttgctgga catcctggcc
    19681 gatgaaaaag agaacggtcc ggaagatacc acgcaagatg acgatatgaa gcagagcatc
    19741 gtcaaatggc tgttcgagct gaacgccaaa cagcgtgaag tgctggcacg tcgattcggt
    19801 ttgctggggt acgaagcggc aacactggaa gatgtaggtc gtgaaattgg cctcacccgt
    19861 gaacgtgttc gccagattca ggttgaaggc ctgcgccgtt tgcgtgaaat cctgcaaacg
    19921 caggggctga atatcgaagc gctgttccgc gagtaagtaa gcatctgtca gaaaggccag
    19981 tctcaagcga ggctggcctt ttctgtgcac aataaaaggt ccgatgccca tcggaccttt
    20041 ttattaaggt caaattaccg cccatacgca ccaggtaatt aagaatccgg taaaaccgag
    20101 aatggtcgtt aacactgtcc aggttttcag accgtctgct accgacaacc ccagatattt
    20161 ggtcacaatc cagaaccctg agtcattaat atgtgacgcg ccaagcccac caaagcaggc
    20221 tgccagcgtc accaatacgc actgaatcgg attcaatccc atcaccgctt ctgagagtaa
    20281 cccgccggtt gtcaggattg ctacggttgc tgacccctgc gatgcacgca gagccagtga
    20341 aataataaat gcggctggta acagaggcag gtcaatcatt tgtagcatat tggcaagggc
    20401 tttgccgacg cccgattcca ccagcacttt gccaaatacc cctccagcac cagtaaccaa
    20461 aatcactacc gccgcagtgg gaagggctga gcccataatg tcgctggtgt gttgtaagct
    20521 ccagccgcga cgtaaagcca ataaccagaa tgccagcacc agcgcaatca ttagagctac
    20581 cattggtgag ccgatcagct gtagcgtacc aagcagggga tgcgaaggcg gcatcagtgt
    20641 tgcggaaacc gtacccgcca tgataatcgc gataggaata acaattagcg aggtgaccag
    20701 cgcgacgccc ggtggattta ttttatcgct taattttgtc gcgccttcct cactggccgg
    20761 agccagttgc atctgttcca gtacttccac cgacatcgca tattggtgct tattgattat
    20821 tttcgctgca aagtagccaa caatccctac gggaatagaa atcgcaatac cgatgatggt
    20881 tagccagccg atgtctgcgt ggagtaaccc cgctgcggcg acagggcctg gatgcggcgg
    20941 taccgccaca tgtacagtta gcatgatccc agcgacaggc aggccaaatt tgagtggcga
    21001 tattttggca accttggcaa aaccgtaaat gattggcgca agaataataa agccgacatc
    21061 aaagaagacg ggaataccga ggaagaacgc tgccagagtc agcgcagcga tagttcgttt
    21121 gtcacctaac ttgcgactga aataattagc cagtgactct gcaccaccag agtgttcgat
    21181 catacgcccc agcatagcgc ccagaccaat aataatagtg acggaaccaa gcacaccgcc
    21241 catcccggcg atcatcactt tacccacttc gcccgccggt atacccgccg caagtgcgac
    21301 taacaggctg acgaggagca gagcaacgaa tggttgtacc tttgccttga tgaccagcag
    21361 caacagcatg attacgccag ttaacgcaat gcataacaat gtaattgtgg acatgggaaa
    21421 ccctgtctga aagttatagt taacctaccc catccgtaga tggggggatg tatgggtacg
    21481 ttgtaattag ggatttaacg aattagcgcc aggcgtcaaa ccagccaagc ccttcttcgg
    21541 tgaggccacg aggtttatat tcacaaccga tccagccctg atatcccacc tcatcgaaca
    21601 ggcggaacag ccacggatag ttgatttctc catcgtccgg ttcatgtcga tcaggtagtc
    21661 cggcaatttg tacgtgcgca tatttcccgg cgtagtcgcg gattaaatgc gtcaggttgc
    21721 catctacttt ttgcgcatga aaagtatcta gttgaataaa cacgttatct cgcgcaacct
    21781 cttcaacaat agccagtgcc tgatactggc tggagaagag ataatgaggc ttaacgtcgg
    21841 ggctgagtgc ttcaactaat attcgcttgc cgtgtagcgc aaagcggtcg gcagcgtagc
    21901 ggagattatc gataaatact gcccggtacc gttcagcgtc ttcgccagcg ggcacgacgc
    21961 ctgccatcac atggacttgt tcacaattga gcgccaatgc atattccagt gccaggtcga
    22021 tgtctgcgtg tgcttcgtgc tcacgtccgg gaagggcgga taatccccat tcccccgcat
    22081 taatatctcc gggagcggta ttgaacagcg ccagtgtcag atggttttgc tccagttgct
    22141 tttggatttg cagggtggag tagtcatagg gaaacagaaa ttccacagca tcgaacccgg
    22201 cttttcgcgc tgcggcgaag cgttcaataa aaggcacttc ggtgaacatc atggataaat
    22261 tagctgcaaa acgaggcatt gcattaactc cttaattccg caatttcacc tgcggtcaga
    22321 taacggatcg ggcggtcacc gagaataaaa atcagctttg ccgtttcctc cagctcttcc
    22381 atattgttgg cggcttcttg caggctttca ccgcaaacca ctgggccatg atttgccagt
    22441 aaaaaagcct gattgtctgc tgccagttcc gccagatcct gtgcgatgcg tttatcgccc
    22501 ggtcggtaat aaggcaccag cgggacattt cccatccgca tcaccacgta tggtgtgaac
    22561 ggacgaataa cgttgctgct gtccagccct tgcaggcagg aaagcgccgt cgaccatgtg
    22621 ctgtgcaaat gcaccaccgc tttacagcgc ggattgttgc gatacagcgc cagatgaaag
    22681 agcacctctt tcgagggttt gtcaccactt aaccattcgc catccgcggc gactttggaa
    22741 agccgctgcg gatcgagatt gcccaggcat gaacctgtcg gtgtcgccag taaattcccg
    22801 tcaggtaaaa gcagcgacag attgccagcc gaaccggttg catagccgcg ctgaaagaat
    22861 gaactggcaa tccgcgtcat ctcctctcgc aaagactgct ctacttttgc gaaatcgctc
    22921 atgataaaaa ctctctttgg gctcgtgaaa aaaaggcttc atcaccgaag ttgccagatt
    22981 taagggcgag tgagacaggc ttatccagtg cgtttaccca cggcacgccg ggggaaatgg
    23041 ttgggccaat atgaaaccct tttatgccca ggctctgtgt gactacgccg gaggtctcac
    23101 cgcctgcgac aataaagcgt gtcacgcctt ccgctgctaa ccgcgccgct agttgagaaa
    23161 acagagtttc tactgcctga ctggcttttt gtgcaccgta ttgctgttga attgctgcca
    23221 atgcgtcagt gctggcggtg gcaaaaacca gtggagcaag tacactttcc tggcccagaa
    23281 cccactctgc cagttcgtgt gcataagcgg ccagagtttc ggttgagagg cagcgtgcca
    23341 catcaacttc acgggctggt gcaatttgac ggtaatgtgc cacctggcgg ttggtcattt
    23401 gagagcatga accggagagc actacgccgc gcccagcgag cggatgccct gcttcgcgag
    23461 cctggttacc gttttcttgc gcccactgcc gggccaggcc aatcgccaga ccagaaccgc
    23521 ccgttaccag tggggcatcg cgcaaggctt ctccctgaat ttccagatgg tgttcggtca
    23581 gcgcatcaag caccgcgtag cggtagccct cttgctgtaa gcgagccagc tcttgacgaa
    23641 cggcatccac accttgttcg aaaacatgtg ccgaaacgac gccgcagcgc cctgtggatt
    23701 gcgcttcaac cagacgggga agatagctgt cggtcatggg atttaccggg tgatggcgca
    23761 tcccggattc ggccagcagt tgattcatta cgaacaaata cccctgataa accgtacgtc
    23821 cgttgaccgg cagggccgga gagaagaccg taaacggcgt gtcgagagca tccattaatg
    23881 catcggtaac cgggccgata ttacctttcg ccgtactgtc gaaagtagag cagtatttga
    23941 aatagatctg tttgcaacct tgctgttgca accagctcag agccgccagc gattgctgtg
    24001 tggcttcaac caccggacag gagcgcgttt tcaggctgat caccagtgcg tcgattgctt
    24061 ccggcatttt acctgttgga acaccgttaa tttgtaccgt tggtagaccg ttttccacca
    24121 gaaaactggc gatatccgtc gcgccggtaa aatcatcggc gataacgcca atcttgatca
    24181 tgatttcgct cccggtagag tgatgccaga gaaaatcttg ataactgcgc tatcgtcttc
    24241 tttcccgtaa cctgcgttac tggcgctggt gaacatattc aatgctgttg aggccaatgg
    24301 cagcgggaag tgcagggctt tggctgtatc ggcaaccaga ccaagatcct taacaaaaat
    24361 atcgacggct gaatgcgggg tgtaatcgcc atccaccaca tgacgcatcc ggttttcgaa
    24421 catccaggaa tttccggcgg cattggtcac gacgtcatac atcacatcca gcgggatccc
    24481 cgcacgggct gcaagtgcca tcgcttcggc tccggcagca atatgtacgc ccgctaacaa
    24541 ctggtgaata atttttacgg tcgaacctag tcccggttct gcacctatgc gataaacttt
    24601 tccggcaacg gcttccagca cgggtgccag tcgttcaaag gcaatatcgc taccggaggc
    24661 catgacagtc atttcaccgt tagcggcttt tactgcacca ccagaaactg gcgcatccag
    24721 catttccaga tcgaatccag ccagagcggt agcaatttct tgcgcatcag cactagcgat
    24781 agtggaagaa accattactg ccgtaccggg tttcagatgt tgtgcaacgc ctgtttcacc
    24841 aaacagcacc tgtttaacct gggccgcatt gaccaccagc accagcagag cgtccagttt
    24901 ttcggcaaac gtcgcggcgt tatcagaaac cccgcaagca cctgcctctt tcaacgtagc
    24961 gcaggcattg ctgttcaggt ctgcgcccca ggtagaaaga cctgcgcgga cacatgacag
    25021 tgctgctccc attcccattg accctaagcc aacgataccg acatgaaact cagatcccgt
    25081 tttcatctgc tctccttgtt aatttaagtg atattttgtt tgatattgtg aatataagcg
    25141 ctggaagata acgatatggt gagctgattc acataaatta acattgtgtg ttattttatg
    25201 tgaactaagc gttagttgcg ccgcgcacgt ttcgcaggca aatagcgtag aatgtcagca
    25261 ggacaaagga aggagcaaaa gttgataccc gtagagcgtc gccaaatcat ccttgagatg
    25321 gtagctgaaa aaggcattgt cagtattgct gaactaacgg acagaatgaa tgtgtcacat
    25381 atgaccattc gtcgggattt acaaaaactg gagcagcagg gagccgttgt gctggtgtcc
    25441 ggaggcgtcc agtctccggg acgcgtggcg catgaacctt ctcatcaggt aaaaactgcg
    25501 ctggcaatga cgcaaaaagc ggctattggc aagctggcgg caagtcttgt tcagccggga
    25561 agctgtatct atctggatgc gggaacgacc acgttagcga tagcacagca tctgattcac
    25621 atggagtcac tgactgtggt cacaaacgat ttcgttattg cgaactactt gctcgacaac
    25681 agtaattgca caattattca cactggcggt gcagtgtgtc gggaaaaccg ttcctgtgtc
    25741 ggggaagccg ctgcgaccat gctgcgcagc ctgatgattg atcaggcttt tatttctgca
    25801 tcgtcatgga gtgtgcgggg gatttctacg ccagcggaag ataaagtcac ggtgaaacgg
    25861 gcgattgcca gtgccagccg ccagcgagtt ttggtctgtg atgcgacgaa atatggtcag
    25921 gtggcgacat ggctggcgtt accattaagc gagtttgatc agattatcac agacgacggt
    25981 ttgccggaga gtgccagtcg cgcgctggcg aagcaggatc tctctttgct ggtagcgaaa
    26041 aatgaataat ggcctgcaat aacatttggt tactcatgct tcacagaaga agcatgagac
    26101 tactttattt tataaaatga cagccgcccg cttttcggcg tgccggtatc aatataaatc
    26161 tggttagcga acgtctgaat gttatcaaac atcatatgtc caaatataaa ataatcagcg
    26221 ccgtttattt gttgtaactc gccattaagc gatttctgca cacgatcaac aggccagagt
    26281 aattcgctct ccgctatttc tttaccaaag agatattcac tccccggata atctgcatgt
    26341 gcgatgacat attttatgtt gtcgttagtg atttcaataa tatgtggaag gtgatggaat
    26401 ttcagcaaca gatctattgc ctcttgttgc tctgaatcat ttaaatcgaa aaaccagtca
    26461 ccaccgctgg caagccacat attgccatcg ccagtttcga atgcatccaa cgccatcgct
    26521 tcgtggttgc ctttaaccga cgtaaaccag ggttggttta gcaggcgcag cacgttaaga
    26581 ctcttcggcc cacgatcaat attatcgcct gtggaaataa gtaagtcggt ttcagggtaa
    26641 aaagagagtt gatgtaaacg ggattgtaat aactgatatt caccatgaat atcaccaacg
    26701 acccatatat ggcgatagtg atgggcattg attttttgat agcgtgtaga tggcatggtt
    26761 ttaccctgta aaataagctt tcctattata cagggtattt ttatttgatt cgtcagttgt
    26821 cgttaatatt cccgatagca aaagactatc gggaattgtt attacaccag actcttcaag
    26881 cgataaatcc actccagcgc ctgacgcgga gtcagtgaat ccgggtcgag attttccagt
    26941 gcttcgaccg caggcgaagt ttcttctggc actgacagca aagacatctg cgtaccatcc
    27001 acttgcgtcg cggcggcgtt cggcgaaatg ctttccagct cacgcagttt ttgccgtgcg
    27061 cgcttaatca cctcttttgg cacgcccgcc agagctgcaa ccgccaggcc gtagcttttg
    27121 ctcgccgcgc catcctgcac gctatgcata aaggcaatgg tgtcgccgtg ctccagcgca
    27181 tcgagatgca cgttggcgac gccttccatt ttctccggta actgggtcag ctcgaaatag
    27241 tgggtagcaa ataacgtcaa tgccttaatc ttattcgcca gattttccgc gcacgcccac
    27301 gccagcgaca gaccatcgta ggtggacgtt ccacgcccga tctcatccat taacaccaga
    27361 ctgtattcgg tggcgttatg taaaatattg gcggtttcag tcatctccac cataaaggtt
    27421 gagcgcccgg aagccaggtc atctgccgcg cctacgcggg taaagatgcg atcgataggt
    27481 ccaatctcga ctttttgtgc cggtacatag ctgccgatgt aggccatcag cgcaatcagt
    27541 gcggtctggc gcatataggt acttttaccg cccatgttcg ggccggtaat aatcaacata
    27601 cggcgctgcg gcgacagatt cagcgggtta gcgataaatg gctcattcag tacttgttca
    27661 actaccggat ggcgaccttc ggtaatgcga atgcccggtt tatcaatgaa ggtcgggcag
    27721 gtgtagttca gggtataggc ccgttccgcc aggttaacca gcacgtcgag ttccgccagc
    27781 gcgctcgcgc tctgttgcaa cgcttccaga tgcggcaaca gcaggtcgaa cagctcttca
    27841 taaagctgtt tttccagtgc cagtgctttg ccttttgagg tgagaacttt atcttcgtac
    27901 tcttttagct ctggaatgat gtagcgctcg gcgtttttca gcgtctggcg acgcatgtag
    27961 ttgatgggtg ccagatggct ttgcccacgg ctgatttgaa tgtagtagcc gtgcaccgca
    28021 ttaaagccaa ctttcagcgt gtccaggccg gtacgttcac gctcgcggac ttccagacgc
    28081 tccagataat cggtcgcgcc gtcagccagc gcgcgccact catccagctc ttcgttatag
    28141 cccgatgcga taacaccacc gtcgcgtacc agcaccggcg gtgtgtcgat gattgctcgc
    28201 tccagcagat cgcgcagctc ggcaaactcg cccatcttct cacgtagcgc ctgtaccggt
    28261 gcactatcga cagtttctaa ctgcgcacgc agctccggca gttgctggaa agcgtggcgc
    28321 atacgggcca gatcgcgtgg gcgagcagtt cgtaaagcca gacgtgccag aatacgttcc
    28381 aggtcgccga cctgacgcag taccggctgc aactcggcgg tgaaatcctg caatgcgcca
    28441 atagtttgct ggcgctcaag caacacgcgg gtatcgcgca ctggcatatg cagccagcgt
    28501 ttcagcatac gactacccat cggcgtgacg gtgcagtcga gcacagaagc cagcgtattt
    28561 tccgcaccgc ccgccaggtt ctgagtaatt tccaggttac gacgtgtcgc ggcatccata
    28621 atgatgctgt cctgctcacg ttccatggtg atagaacgaa tatgcggcag ggtcgtgcgt
    28681 tgggtatctt tcgcatactg caacagacaa ccggcagcac aaagtccgcg cggcgcgttc
    28741 tcgacgccaa aaccgaccag atcgcgggtg ccaaattgca gattcaactg ctggcgcgcg
    28801 gtgtcgattt caaactccca cagcgggcga cggcgcaggc cgcgacggcc ttcaatcagc
    28861 gacatctcgg cgaaatcttc tgcatacagc agttccgccg gattagtgcg ttgcagctct
    28921 gccgccatcg tttcgcggtc ggccggttcg ctcaggcgaa aacgcccgga gctgatatcc
    28981 agcgtcgcgt agccgaaacc tttgctgtcc tgccagatag ccgccagcag gttgtcctga
    29041 cgctcctgta acagggcttc atcgctgatg gtgcctggcg taacgatacg cacaactttg
    29101 cgctcaaccg gacctttgct ggtcgccgga tcgccaattt gttcgcagat ggcaacggac
    29161 tctccctgat tcaccagttt ggcgagatag ttttccaccg catggtaggg aatccccgcc
    29221 atcgggatcg gctctcccgc cgaagcaccg cgtttggtca gtgaaatatc cagcagttgc
    29281 gacgcgcgtt ttgcgtcgtc ataaaacagt tcataaaaat cacccatccg gtaaaacagc
    29341 aggatctcgg gatgctgggc tttcagcctg agatactgct gcatcatggg cgtatgggcg
    29401 tcgaaatttt ctattgcact catggggtta tgtccggttc cctgatatta aatggggtgt
    29461 gatggttttt attttcgttg taatcacata aggcgcacat gataccggag ttaatcattt
    29521 caggggaaga agcgaacggc gagttcttga tgctggttcc gggaaagatg ctgttttgca
    29581 gcttttgcgt gatgcttatg aagcaagtac gcaaaattgc gtcggttgaa taaacctcct
    29641 ccttcagagc catagtcccc tttcaaatta agttgtaata taaatacacc ttaagagtat
    29701 ggaggcagca tgtccggcaa gcgtatctct cgtgaaaaac tgacgattaa aaaaatgatc
    29761 gatctttatc aagcgaaatg cccgcaggcg tcagcggagc cggagcatta cgaggcgttg
    29821 tttgtttacg cgcaaaagcg gctggataaa tgtgtgttcg gcgaagagaa accagcctgt
    29881 aagcagtgtc cggtgcactg ttaccagcca gcaaagcgtg aagagatgaa acagattatg
    29941 cgttgggccg ggccgcgaat gctgtggcgt catccgatct taaccgtgcg tcatctcatc
    30001 gatgacaagc gtccggtgcc agaactgccg gaaaaatatc gtccgaaaaa aactcgtgaa
    30061 tagtatgccg gacaggcaga tctgtccggc aatttgcagt taaatcaatg ccgatttatc
    30121 aattccaagc cgcttcatcc gtgacagcaa ggtcgtgcgt ttcagcccca gacgttgcgc
    30181 agcgccttta ggcccggcaa ccacgccgtt ggtttctttc agcacgcgaa caatcaactg
    30241 atattcatct tcgccctcct gggcgacaac cgttgcggca ggcggcgttt caggttccgg
    30301 taaaacaata tctggcaatg acagctgcag cacgttaccg cgtgttagca ataccgcgcg
    30361 ctcaatgacg ttttccagtt cgcgtacgtt acccggccac tccatgttgc tcaaggtgcg
    30421 cagcgtctcg gcaggaatgc tgtcgatatt gcgccccaga cgacgggcaa ttttgaaggt
    30481 aaaggctttc gccagcagcg gaatatcttc cggacgctcg cgtagtggcg gcaggtgaat
    30541 cgggaatacg ttcaggcggt aatagagatc gctacggaac tcacggtcgg cgaccatttt
    30601 tttcagatcg cggttagtcg cggcgattag acgcacgtcc gtctgaatga ttttgttgct
    30661 gccgagacgt tcaaactcct gttcctgcaa tacacgcagc aacttcggct gtaactccag
    30721 tggcatatcg cccacttcgt cgaggaacag ggagctttta tccgccagtt caaaacgacc
    30781 aatgcgctgg gcgctggcac cggtaaaagc cccacgctca tgaccaaaca gatcgctttc
    30841 cagcaatccg gcaggcatcg ccgcgcagtt cattttgacc atgcggcgat tattacgccc
    30901 actgagatta tggatcgcac gggcaatcag ctctttaccc gtgccagttt caccgaggat
    30961 cagcacggta ctgtcacttt gcgccaccat ttcaacttgt ttaagcacgc tgtacatggc
    31021 ttcgctgcgg ccaataatct cgccaaattc actatcaaca ttgttgagct gctcggtcag
    31081 ggcgaggttt tcatcaacca gccgttcttt cagacgatgg atttcctgat aggcgagggc
    31141 gttatcgaca gcgattgcca cacgttcggc aatctggcgc agtaaattca gattggtagt
    31201 ggtaaacact ttctcttcgc attgcgccag tttcagcacg cccagcatgg tgtcgccaga
    31261 catcagcggt aacaggcaca aggtttgaat ctggttgccc caggtgtcga acaacatgcg
    31321 ttcatagggg gctaaatcgt cccgctcgtg gagattgatc agcagcatct ctttactttt
    31381 gaacacgcgt tcggtgaggg ttccggcttc atcgacttcg ctctgttcgt gggcgggatg
    31441 ttgtttatca agatagtgag tggagtagat gttgagtttg tttttacggt ggctgcgtaa
    31501 gacaatgctg atatcgtcaa tgtcgaaata gtaatggatt tctttggcga cttcgctgac
    31561 cagttcgtcc atatccaggc gggaaagcac cgcgttggta atggcaacca ggatgcggaa
    31621 gttatcgcgc tcccggcata acaactcata gtcgacattg ttgacgacgc ggctctggat
    31681 ttgttcggtg acgacagaaa cgatctgcgt aaatgtttgc agacgattga actctttttc
    31741 gctccagggg cgatcgtcat aacgaataaa ttcacagcca ccaaaaatat gcccttccgc
    31801 cgccagtggc atcaggcaat agtgaccaaa tttggggtat agcccacctg cggccagctg
    31861 cggccaggtt tcacaaaatt cttcgtaact gcaatgcagc gtatcagggc gcgacaaaat
    31921 gctgcgtacc ggaccgtgtg ccagaacagt ttcgtcttca tatttaatgg gggtgtcttt
    31981 ttcacgcgat gcgtaataag acgcacgttg agtctgcgct tgccacaaca caatagccgc
    32041 gttgtcggcg agcgcagaac gctttaccag ttgcgaaaga gcctcacaca gcgaggccag
    32101 atcgggctgc tgcaatagtg tccgagtgat gtcgaacaac ccttgctgtc cgagatcact
    32161 catcggtgta tatgacattt tgttcttcca ggaaagcacc gcaaagcggt gcaaaaggtc
    32221 attgactaac tataggagat ttagaatttt attagcatat acgcggaagc ggttcggcgt
    32281 gtggtaaatc gagggttcgt ttcacgccat acagaccggc aagacgaaca cctttacgtt
    32341 ccaccacttc accaatcagc gccgcgtctt tccccagtgg atgggaatgt aacgctgcca
    32401 gcacttgctc tgccgcgttg cgttcaacag ctattactag tttgccttcg ttggcaaagt
    32461 tcagggcgtc cagtcccagc aattcgcaaa cgccacgcac ggcaggttta acaggcagtg
    32521 ccgcttctga aagttcaata ccacaaccgc aggctgccgc gaactcatga accaccgcgt
    32581 ttacaccacc acgggtggca tcacgcagcg ctttcacgcc gggaatgtca cgcagcgtct
    32641 gaataagcgg cgtcagcacc gcgcagtcgc tgaccagttc gccatccagc cccagctgct
    32701 cacgcaggtt aaggatagtc gccccgtggt cgccgagtgt accgctcacc agcaatacat
    32761 cgcctgcggt tagcgtctgt gcgccccagt gaatattcgc cggaattgcg cccatgccag
    32821 cggtgttgat aaacagttta tctaccgcgc cgcgctgcac cactttagta tcgccagtaa
    32881 cgatggcaat gcctgccgtg cgggcggttt ctgccatgct ggtcactacg gcttttagtg
    32941 tctccatcgg caatccttct tcgaggataa agccacagga gagatagcgc ggaatagcgc
    33001 cactgaccgc aacgtcattc gcggtgccgc aaatcgccag cttgccgata ttaccgccag
    33061 gaaagaacag cgggtcaatg acgtaactgt cggtggagaa cgccagacga tcgccttctg
    33121 ctaccagctg cgccagatca agacgtgcct ggtcttcctg ctctgccagc cacgggttgg
    33181 caaaggcttc cataaacagg ctgttgatta attgctgcat cgcctggccg ccgctgccgt
    33241 gggcgagttg gatattattc acgcttcatt ctcctgctga cgatactgat accacgcggc
    33301 gcacgctcct tcggaggaga ccatcagtgc accaaatgcg gtttgaggat tacaggtgtt
    33361 accaaacagc gggcattgat gcggcttaca tttgcccgtt aatacttcac cacaacgtgc
    33421 gcgcgggtca tcgcagacct gctgcggtgc cgggcggaaa tgtgcttcgg catcgaatcg
    33481 ttgatagtcc ggcgtcaggt gtacaccaga agattcaatc acgcctaagc cgcgccattc
    33541 gctgtcgccg ttgacgcaga agacatccgc aatcgcctgt tgcgccagca ggttaccggc
    33601 atccggcact acccgccgat actgattttc taccttgcta tgagcagcta ttttctgctc
    33661 caccagcatg accacgcctt gtagtagatc aaggggttcg aatccagcaa ccaccagcgg
    33721 acgatgaaaa tcgctggcga taaaattata ggcgttggtg ccgataacca tactgacgtg
    33781 acctggcgcg aggaacgcat cgataccgtt atccggctgt tccagcaaac tgcgcaacgt
    33841 cgggataagc gtaatgtgct ggcagaagaa gtaaaaattc tgcacatcac gcgctttcgc
    33901 ctgttgcaga gtgatggcgg tggtcggcat ggtggtttca aaacctaagc cgaagaacac
    33961 cactttgcgg gttggattct cctgcgccag tttcaacgca tccattggcg agtaaacgat
    34021 gcgaacatcg gcaccgcgtg cttttgcctg caacagcgat ccctgtttcc ccggcacgcg
    34081 catggcgtcg ccaaaggtac agaagatgac ttccggatgg ctggcaatct ccacgcaggt
    34141 gtcgattcta cccatcggca gtacgcacac cgggcaaccc ggaccgtgga taaactcaac
    34201 gttttcaggc agtaactggt cgaggccgaa tttaaaaatg gcgtgggtat gaccaccgca
    34261 cacttccata atccgcagag ggcgttcggc tgtgtaagag agatgtgaag cacgttcgcg
    34321 cagatgctca attaactgca tcacctgttc cggcgcgcga tattcatcaa caaaacgcat
    34381 tatttttcct cgccatacaa cagcgcgccg acatccggct caacgtcaaa catgttttgt
    34441 aaggcgtcga gagtgtcgcg tgcttcggct tcattaatta cgctcatggc aaagccaacg
    34501 tgtaccagta cccactggcc cacgcgcggc tgaccgtttt catcgcagct gccgactaac
    34561 gttaaatcga catcgcgctg aatgccgcag acgtcgactt tcgcctggtt gccgtcaatg
    34621 gtgcggatct ggccgggaac gcctatgcac atcgctgtgt ctccagccag ttcagccact
    34681 ggtccatccc ttcgccgctg gtggcggaaa taaggatgat ttcaatttct ggattgactt
    34741 cgcgggcgca ggcgatgcac ttctcaacgt caaagttgag atacggcagc aggtcaactt
    34801 tgttgagcag catcagcgag gcggcggcaa acatatgcgg atatttcagc ggtttgtctt
    34861 caccttcggt aacggaaagc accgccactt tgtgtttttc accgagatcg aagctggccg
    34921 ggcatacgag gttgccaacg ttttcgataa acagaatacc gttatcgtcc agtggcaggc
    34981 gcggtgcggc ttcggcaatc atctgtgcgt caagatggca gcctttaccg gtattcacct
    35041 gaatcgctgg tgtgccggta gcgcgaatgc gtgcggcatc gttcacggtt tgctgatcgc
    35101 cttcaataac tgcgcacgga acgctgtctt tcaggcgcat tagggtttcc gtcagcaggg
    35161 tggttttacc ggaaccaggg ctggaaacca ggttgagcac cagttgcttg cgggcagcaa
    35221 agcgcgcgcg gttgcgttca gccagacggt tatttttgtc cagcacgtca atttcgactt
    35281 ccagcatccg acgctggctc atgcccggcg cgtgagtgcc tgcttcgcca tgaccgtaat
    35341 gcaggtcgcc ttcttcagtc tggctggggg taaattcagg cgctttaatg ccggtgattt
    35401 tcatcttcgg gcgtgccgcc ggggcaaatg gcgcgctacg aaacgcggaa tgagggttat
    35461 gttcatcacc ctcgatatac aggttgcctt caccgcaacc gcatgttgta cacatcgctc
    35521 actcctggtc tatttctatc cgccgaatct gtaaaccgtc gtctgccaca atctgtagca
    35581 tgtcaccatg acactgtgga cagcggcgga cgcgctgggt cagtagcgtc acatactgtt
    35641 ggcatgtttc acaccagcat tcggcctctt gttcttcgag gtgcagttta caaccttccg
    35701 ccacgctgcc gcggcaaacc agatcaaaac aaaaggcaag agagctggtt tcgacacaag
    35761 aaaatgcgcc aattttgagc cagaccccag ttacgcgttt tgcgccgtgt tttgcggcct
    35821 gctgttcgat caattccagt gcccgttggc agagggttat ttcgtgcata tcgcctccca
    35881 ttaactatgg ccagctacaa gcaataattg tgccagtatt gattatccct gcggtgaata
    35941 atgtcgatga tgtcgaaatg acacgtcgac acggcgacga aattcatctt tagcttaaaa
    36001 atcgttttaa taacaatgaa ttaaaagttg gcacagaaaa tgcttaaagc tggcatctct
    36061 gttaaacggg taacctgaca atgactattt gggaaataag cgagaaagcc gattacatcg
    36121 cacagcggca tcgtcgccta caggaccagt ggcacatcta ctgcaattcg ctggttcagg
    36181 ggatcacgtt atcgaaagcg cgcctgcatc acgccatgag ctgcgcgccg gacaaagaac
    36241 tctgtttcgt cctttttgaa cattttcgca tttacgtcac cctggcggat ggctttaaca
    36301 gccacaccat cgagtattac gtcgaaacaa aagatggcga agacaaacag cggattgcgc
    36361 aggcgcaact gagcattgac ggcatgattg atggcaaggt caacatccgc gatcgcgaac
    36421 aggttctgga acactatctc gaaaaaatcg ctggcgttta cgacagctta tacaccgcta
    36481 ttgaaaacaa tgtgccggtg aatttaagcc aactggtaaa gggacaaagc ccggcagcat
    36541 gagctgaggc tttgcccgtt ttgcaggcgt tacgcctgtt tggggatggg cgtgtcgatg
    36601 agtgtcgaaa atgacatttc atcggcatgt tttcgtcaaa aatgacaatc acctgaggaa
    36661 tgcctggtga atcgttttgt aattgctgac tccacgctct gtatcggctg ccacacttgt
    36721 gaggccgcct gttcagagac gcatcgccag cacggcctgc aatcaatgcc gcgcctgaga
    36781 gtgatgctga atgaaaaaga atctgcgccg cagctctgtc accactgtga agatgcaccc
    36841 tgcgcggtgg tctgcccggt taacgccatc acccgcgttg atggggccgt gcagttgaat
    36901 gaaagcctgt gcgtaagctg caagctgtgc ggcatcgcct gcccgtttgg cgcaattgaa
    36961 ttttccggca gccgtccgct ggatattccg gcaaacgcca ataccccgaa agcgccaccg
    37021 gcaccgcctg ctccggcgcg tgtcagcaca ttgcttgact gggtgccagg tattcgcgcg
    37081 atcgccgtca aatgtgacct ttgtagcttt gatgaacaag gtccggcctg cgtgcggatg
    37141 tgcccgacta aagccctgca tctggtggat aacactgata tcgcccgcgt cagcaaacgt
    37201 aagcgtgagc tgacttttaa cacggacttt ggcgatctca ccttgtttca gcaggctcaa
    37261 agtggagagg ctaaatgagc gcaatttccc tgatcaatag cggcgtggcg tggtttgtcg
    37321 ccgccgctgt tctggcattt ctcttttctt ttcaaaaagc gttaagtggc tggatagctg
    37381 gaattggcgg cgcggttggt agtctgtata cggcagccgc gggcttcact gtactgactg
    37441 gcgcggttgg cgtgagcggt gcgctgtcgc tggtaagcta cgatgtgcaa atctctccgc
    37501 ttaacgcgat ttggctgatt acgctcggtc tgtgcggtct gtttgtcagc ctctacaaca
    37561 ttgactggca tcgccacgcg caggtgaagt gcaacggctt gcagatcaat atgttgatgg
    37621 ctgccgccgt ctgcgccgtc attgccagca acctcggcat gttcgtggta atggccgaaa
    37681 tcatggccct gtgcgcggtg ttcctcacca gcaacagcaa agagggcaaa ctgtggtttg
    37741 cgctggggcg tcttggcact ctgctgctgg cgattgcttg ctggctgctg tggcagcgtt
    37801 atggcacgct ggatctgcgc ctgctggata tgcgtatgca acagctgccg ctcggttccg
    37861 atatctggct gctcggtgtg attggctttg gcctgctggc cgggattatt ccgctgcacg
    37921 gctgggtgcc gcaggcacat gctaacgcct ctgcgccagc tgccgcgttg ttttctacgg
    37981 tagtcatgaa aattggcctg ctgggcattt taaccctgtc actgctgggc ggtaatgcac
    38041 cgctgtggtg ggggatcgcg ctgctggtgc tcggcatgat caccgcgttc gtcggtggtc
    38101 tgtatgcgtt gatggaacac aacatccagc gcctgctggc ttaccacacc ctggaaaata
    38161 tcggcattat cctgctgggg ctgggcgctg gcgtaacggg tatcgcgctc gaacaaccgg
    38221 cactgattgc tcttggcctg gtcggtggtc tgtaccatct gcttaaccat agcctgttca
    38281 aaagcgtact gttcctcgga gcggggagcg tctggttccg taccggtcat cgcgatatcg
    38341 aaaaactcgg tggtattggc aagaaaatgc cggttatctc catcgccatg ttagtcgggc
    38401 tgatggcaat ggctgcgctg ccgccgctga atggttttgc cggggaatgg gttatctatc
    38461 aatccttctt caaactgagc aatagtggcg cgtttgttgc ccgtctgctg gggccgctgc
    38521 tcgccgtggg tctggcaatt accggtgcgc tggcggtgat gtgtatggcg aaagtctatg
    38581 gcgtcacgtt cctcggcgcg ccgcgtacca aagaagccga aaacgccacc tgtgcaccgc
    38641 tcctgatgag cgtaagcgta gtggcactgg cgatttgctg cgtaattggc ggtgttgctg
    38701 cgccgtggct gctgccgatg ctgtctgctg ctgtacctct gccgctggag cctgctaaca
    38761 ccaccgtttc tcaaccgatg atcacgttgc tgctgattgc ctgcccgctg ctgccattca
    38821 tcattatggc gatttgcaaa ggcgatcgtt tgccatcgcg ttcccgcggc gcggcctggg
    38881 tgtgcggcta cgaccacgaa aaatcaatgg tgattaccgc tcacggtttt gccatgccgg
    38941 tgaaacaggc gcttgcgcca gtgctgaaac tgcgcaaatg gctgaatccg gtgtcgctgg
    39001 tgccgggctg gcagtgcgaa ggtagtgcgt tgctgttccg ccggatggcg ctggttgaac
    39061 tggcggtact ggtggtgatt attgtttcac gaggagcctg agaatgagtg ttttatatcc
    39121 gttaattcag gcgctggtgt tatttgccgt tgcgccgctg ctctccggta ttacccgcgt
    39181 ggcgcgcgcc cgtctgcata accgtcgtgg gccgggtgtg ttgcaggagt atcgtgacat
    39241 tttcaaactg ctgggccgcc agagcgttgg cccggatgcc tccggctggg tgttccgcct
    39301 gacgccgtat gtgatggtgg gcgtcatgct gactatcgct actgcgctgc cggtggtgac
    39361 cgtcggttct ccgctgccgc aactgggtga tttaatcacc ttactgtatc tctttgccat
    39421 cgcgcgtttc ttctttgcca tttctggtct ggataccggt agcccgttta ccgctatcgg
    39481 cgcgagccgt gaagcgatgc ttggcgtgct ggtcgaaccg atgctgctgc ttggtctgtg
    39541 ggttgccgca caggttgccg gttccaccaa catcagcaac atcaccgaca ccgtttatca
    39601 ctggccgctg agccagagca tcccgctggt gctggcgctt tgtgcctgtg cgttcgccac
    39661 ttttatcgaa atgggcaaac tgccgttcga cctggcggaa gccgagcagg agttgcagga
    39721 aggtccgctc tccgaataca gcggtagcgg ctttggcgtc atgaaatggg gtatcagcct
    39781 gaaacagctg gtggtgttgc agatgttcgt cggggtgttt attccgtggg gacaaatgga
    39841 aaccttcacc gccggtggac tgctgctggc gctggtgatt gccatcgtca aactggtggt
    39901 cggcgtcctg gttatcgcgc tgttcgaaaa cagcatggcc cgtctgcgtc ttgatattac
    39961 tccgcgcatt acctgggctg ggtttggctt tgcattttta gcgttcgtct ccttgctggc
    40021 ggcgtgatta aagagagttt gagcatgtct gaagaaaaat taggtcaaca ttatctcgcc
    40081 gcgctgaatg aggcatttcc gggcgtcgtg ctggaccacg cctggcagac caaagatcag
    40141 ctgactgtca ccgtaaaggt gaactacctg ccggaagtgg tggagtttct ttactacaaa
    40201 cagggtggct ggctgtcggt gctgtttggt aacgacgaac gcaaactgaa tggtcattac
    40261 gccgtttatt acgtgctgtc gatggagaag ggcactaagt gttgggtaac ggttcgcgtc
    40321 gaagttgacg ccaacaaacc ggagtatccg tccgtgacgc cgcgcgttcc ggcggctgtg
    40381 tggggcgagc gcgaagtgcg cgatatgtac ggtttgattc cggttggtct gccggatgaa
    40441 cgtcgtctgg tgctgccgga tgactggccg gatgaacttt atccgctgcg taaagacagc
    40501 atggattatc gtcagcgtcc ggcgccgacc accgatgctg aaacctacga gttcatcaat
    40561 gaactgggtg acaagaaaaa caacgtcgtg ccgattggtc cgttgcacgt cacttccgac
    40621 gaaccgggtc acttccgtct gttcgtcgat ggcgaaaata ttatcgacgc cgactaccgc
    40681 ctgttctacg tccatcgcgg catggaaaaa ctggcggaaa cccgtatggg gtacaacgaa
    40741 gtgaccttcc tctctgaccg tgtgtgcggc atctgcggct ttgctcacag caccgcctac
    40801 accacgtcgg tggaaaacgc gatgggtatt caggtgccag aacgtgcgca gatgatccgc
    40861 gccattctgc tggaggtgga acgcctgcac tcgcatctgc tcaacctcgg cctggcctgt
    40921 cactttactg gcttcgactc cggctttatg cagttcttcc gcgtgcgtga aacctccatg
    40981 aaaatggcag agatccttac cggtgcgcgt aaaacctacg gcctgaacct gatcggcggg
    41041 attcgtcgcg atctgctgaa agatgacatg atccagaccc gccagctggc gcaacaaatg
    41101 cgtcgtgaag tgcaggagct ggtggatgtg ctgctgagta cgccgaacat ggaacagcgc
    41161 actgtcggca ttggtcgtct ggacccggaa atcgcccgcg atttcagtaa cgttggcccg
    41221 atggtccgcg ccagcggaca cgctcgcgat acccgcgccg atcacccgtt tgttggttat
    41281 ggcctgctgc caatggaagt ccacagcgag cagggctgcg acgttatttc ccgtctgaaa
    41341 gtgcgtatca acgaagtcta taccgcgctg aacatgatcg actacggtct ggataacctg
    41401 ccgggtggtc cgctgatggt ggaaggcttt acctacattc cgcaccgttt cgcgctgggc
    41461 tttgccgaag cgccgcgcgg cgacgatatc cactggagca tgaccggcga caaccagaag
    41521 ctgtaccgct ggcgctgccg tgccgcgacc tacgcgaact ggccgaccct gcgctacatg
    41581 ctgcgtggca ataccgtttc cgatgcgccg ctgattatcg gtagcctgga cccttgctac
    41641 tcctgtaccg accgcatgac cgtggtcgat gtgcgtaaga agaagagcaa agtggtgccg
    41701 tacaaagaac tcgagcgtta cagcattgag cgtaaaaact cgccgctgaa ataaggaatc
    41761 gccatgttta cctttatcaa aaaagtcatc aaaaccggca cggcgacctc gtcttatccg
    41821 ctggagccga ttgcggttga taaaaacttc cgtggtaagc cagagcagaa cccgcagcag
    41881 tgcatcggct gcgcggcctg cgtcaatgcc tgcccgtcaa acgccttaac ggttgaaact
    41941 gacctcgcca caggagagct tgcgtgggag tttaatctcg ggcgctgcat cttctgtgga
    42001 cgctgcgaag aagtctgccc gacggcagcg atcaaactgt cgcaagagta cgaactggcg
    42061 gtgtggaaga aagaagattt cctgcaacag tcccgttttg tgctgtgcag ctgtcgcgtc
    42121 tgcaatcgtc ctttcgccgt ccagaaagag atcgactacg ccattgcgct gcttaagcac
    42181 aatggtgaca gccgcgcgga aaaccaccgc gaaagctttg agacttgccc ggaatgtaag
    42241 cgccagaaat gcctggtgcc gtccgaccgt attgaactga ctcgccatat gaaagaggcc
    42301 atctgatgag caatttatta ggcccccgtg acgccaacgg cattccggtc ccgatgacgg
    42361 tggatgaatc catcgccagc atgaaggcgt cgttactgaa aaaaatcaaa cgttctgcct
    42421 acgtttaccg cgtggactgc ggcggctgca acggctgcga aatcgaaatt ttcgccacgc
    42481 tttcgccgct gtttgatgca gaacgcttcg gcattaaagt cgttccttca ccgcgtcatg
    42541 cggatatttt actgtttacc ggcgcggtca cccgtgcaat gcgatcccct gcgctgcgtg
    42601 cgtggcagtc cgcgccggac ccgaaaatct gtatctccta cggtgcctgc ggtaacagtg
    42661 gcgggatctt ccacgatctc tactgcgtgt ggggcggtac ggataaaatc gtcccggtag
    42721 atgtctacat ccctggttgc ccgccaacgc ctgccgccac gctgtacggc tttgcaatgg
    42781 cgctcggcct gctggagcag aaaattcacg cccgtggccc gggtgagcag gatgaacaac
    42841 cggcggaaat cctgcatggc gatatggtgc agccgctgcg tgtgaaagtg gatcgcgaag
    42901 cacgccgcct ggcaggttat cgttacggtc gtcagattgc cgatgacttc ctgacacagt
    42961 tagggcaggg cgaagaacag gttgcacgct ggctggaagc ggaaaacgat ccgcgtctga
    43021 acgagattgt cagccatctg aatcatgttg tagaagaggc gcgtatccga tgagtgaaaa
    43081 ggtggtgttc agtcaactga gccgtaaatt tattgatgag aacgatgcca cgcccgccga
    43141 ggcgcagcag gtggtctatt acagcctggc gattggtcac caccttgggg ttatcgattg
    43201 cctggaagcg gcgctcacct gcccgtggga tgaatatctg gcatggattg ccactctgga
    43261 ggcgggcagc gaagcccgcc gcaaaatgga aggcgtgccg aaatatggtg agatcgtcat
    43321 cgacattaac catgtgccga tgctggccaa cgcattcgat aaagcccggg cagcgcaaac
    43381 ttcgcagcag caggaatgga gtacaatgct gctaagtatg ctgcatgata ttcatcagga
    43441 aaacgccatc tatttgatgg tgaggagact gcgtgactga cgttttactc tgtgttggca
    43501 atagcatgat gggcgatgat ggcgcaggcc cgctgctggc ggaaaagtgc gccgccgcgc
    43561 cgaaaggtaa ctgggtggtg attgacggcg gtagtgcacc ggaaaacgac atcgtcgcca
    43621 tccgtgaact gcgccctacg cgactactga ttgtcgacgc cactgatatg gggctaaacc
    43681 ccggcgagat ccgcatcatc gacccggatg atatcgccga gatgtttatg atgactaccc
    43741 ataacatgcc gttgaactac cttatcgacc agttgaaaga agatattggc gaagtgattt
    43801 tcctcggcat ccagccggat atcgtcggct tttactaccc gatgacccag ccgattaaag
    43861 atgcggttga aaccgtttat caacgactgg aaggctggga agggaatggc ggcttcgcgc
    43921 agttagcggt ggaagaagaa taattctcca tcagggaatt cagcgaaatg aatatcagaa
    43981 cagggatgtg cgctttttta ttgagcttag tgctgcctgc acaggccacc agctttaccg
    44041 aatatctgcc aatgagtgac agcgaatacg cgcaaaagcg ggcgctgaaa cctctcctga
    44101 ccatgcctta tgatgccgag caaacctggc attttagaaa agtgggcgtt gctggcgtga
    44161 cgcttgaaaa gatgccaaat gatgatagtg aatggcaatt gaacggtaaa gatcgcgccg
    44221 gtaaatcctg gtctgtgcct gtgggtatgc tggcaaacat agcgggcaaa gggcaactct
    44281 atcgcgccga tcttgatcgt aacggcattc aggatctggt tatctggtta ccaagtaccg
    44341 gtaatggtct ggctccttat gcacatttaa ttcttatgac gttcacgcgc gaaggccgtc
    44401 cctgtgtctt tgaacccagg ggattctaca ccgcaagcaa aactggcgta gatgatttgc
    44461 tggatttgca aggcaatggt cacacgcaat tgctggatat gcagtttaat tctggttact
    44521 ggataaccag tctgtatcag gtaaaagacg ccaaatggca gcgtgttcac ggctggtttg
    44581 gcaagttaag ctatcccgcg ctcacgcgtt ttacttatac ccccaaccgt aaactcgtcc
    44641 tcaaacccat cgccgggcgc gacccgcaaa cggaagatct ggcgcagacg cagcgttgct
    44701 tgataaaggg ggatgtgctt gaggggtgaa ccagaactga ctttttgcag agtggctatg
    44761 aggattttgg gggtaattat atcgcgcaca agtgtttaca tattgtgaac ttggatttga
    44821 tgagctatag tttccgaaca cataaggata gaacatcgca gatgtggctg tttacttact
    44881 atcaggaagt taaaacttaa aaatgcacat catttctaaa gcgccatttg aagagtctgc
    44941 gcgtaaatat cctaacgacg ctctggctct tcaggcattg tatcgggtta ttaaggaaac
    45001 ggatttttct acaccagaag aaatgcgaac ggcgtttccc aacctcgaca attttagata
    45061 tcgcaacaaa tggtacgttc tggatgtcgg cgggaacaat cttagggtta ttgcctacat
    45121 aaactttgtt aataagcggt tctttgtgaa gcacataacg aatcacgcag agtatgacaa
    45181 actgacacgt tactacagag agaacaaaga atgactgcta atgctgcacg cgctgttaaa
    45241 gcaacaagag aactggtcaa tgcagtcccc tttctgggag gcagtgactc agaagatgat
    45301 tatcgtgaag cactggaact ggtcgaatat ttgattgaag aagatgacac caatccactt
    45361 atcgactttc tggcgagccg cattgcagag tatgaaaaca acaacgaaaa gtttgcagag
    45421 ttcgacaaag ccgttgcggc aatgccggtt ggcgtggcgt tacttcgcac gttgattgat
    45481 caacataatc tgacctatgc ggatttgaag aacgaaatcg gctctaaatc cctggtcagt
    45541 cagattttgt ctggacaacg atccctgacg atatcccaca ttaaggcact ttcagcccga
    45601 tttggcgtta agccggaatg gtttctttga ttttgactga tgccaaaccc cagattgccc
    45661 gatgcgctgc gcttatcggg ccttcctggt tcgtgcgaca tgtaggccgg ataaggcgtt
    45721 cacgccgcat ccggcactgt tgcttactct aaatcttcac cattactggc aatcactttt
    45781 ttataccacc agaacgattt cttacgtgtg cgcgtcagcg tgccgttgcc tgcgtcgtca
    45841 cggtctacgt agacaaaacc gtagcgtttg ctcatttcac ccgtagaggc ggaaactaaa
    45901 tcaatacagc cccatgtggt gtagcccatc agcggaatgc catctgcaat cgcttcgccc
    45961 attgcgcgga tatgttcgcg taaatagctg atgcgatagt catcgttaat ctcgccattg
    46021 gcagcaagtt catcttttgc gccgaggccg ttttccacca gaaacagcgg cttctgataa
    46081 cggtcgtaca tcatgttcat ggtgatacgc agaccgagtg gatcaatacc ccagccccag
    46141 tcgctcactt gcagatacgg gttacgcagc gatttcacta cgttcgccgc actgctattg
    46201 ttggcgttca tctccgccga ggcgcagcgc gaggcgtaat agctgaaaga gacaaaatcg
    46261 acggtgtttt tcaggatttc atcatcgccc ggtgctttgt cgatggttac ccctttttcg
    46321 cggaatacgc gggcagagta agccggatac gcgccccgcg cctgcacatc gataaaaaac
    46381 aggttttccc ggtctttctc cagcgccgcc cagacatctt ccggcttgca actgtaaggg
    46441 tagaagttac cgcccgccag catacagccc acctgatttt gcgggttaac ctcatgggcg
    46501 attttggtgg ctagcgcact ggcaaccagc tggtgatgcg cggcctgata tttcacctga
    46561 tcctgatttt caccttcttc aaacaccaga cccgcgccgg agaacgggct atgcaacata
    46621 atgttgattt cattgaaggt aagccagtat ttcaccagac catcaaatgc ttcaaagcag
    46681 gtccgggcgt agcggctgaa aaactctacc agcttgcggt tacgccagga gccatattcg
    46741 gtgaccagat gcatcggcac atcgaagtgg cacaacgtga ccagcggttc gataccgtac
    46801 tttttacact cttcaaagac agaacgataa aaagcaatgc cctgctgatt gggcgtgatt
    46861 tcatcgccct gcggaaagag acggctccag gcaattgagg tacggaaaac cttgaatccc
    46921 atctctgcca tcagggcgat atcttcttta taacgatgat aaaaatccgt cgcctcatgg
    46981 ctgggataaa actcgtcatc gcgcaactga aaacgttttt ccagccccag tttcaccgcc
    47041 attcgatgct cgccgtgtgg gatcatatcg acagtggtca gccctttgcc accttcacgg
    47101 aacgcacctt cagactggtt ggcggcaagc gcgccgcccc ataaaaaacc ttctggaaat
    47161 actgacattt tcatcctcaa ttaagactta cttctttggc gacggtcggt tgtacgctct
    47221 gatgctttcg cgcctcggca gtcgcttcct caacaggaat atcctcgaag ccgagcaaca
    47281 gtgtgaggat aaacgacagc atgaccgcca gcgccatgac cgcgaacacc cagacgatgc
    47341 tcattggatt cgccggatcg aagaactgca cgctggtaaa tagccccggc gctgccattg
    47401 agtggctggc aagccccgcc ataccggcaa ccgcgccgca aataaagccg ctgataagac
    47461 tggcgataag cggacgtttc aggcggatcg ccacgccgta taacgccggt tcggaaatcc
    47521 ccgccataat ggctgatgcc gccgcagcca gcgccgtctg gcgcagttcc gggtttttcg
    47581 ttttccacgc caccgccagt gatgaaccgc ccagcgacag gttagcgccg atctctgacg
    47641 gcatgaccat cccttctttg ccggtttcgg caatggtctg aatgatggtt ggcgtaaaga
    47701 cgcggtgcat cccggtcatt accagcagag gccacagcgc gcccataatg gcgactgaaa
    47761 gccagcccag ataaccatga atggtgtaaa ccagcgccga aatggcgcta ccgatccaga
    47821 taccaatcgg gccaatcagc aggattgcca gcggtgcggc aatcaacaca atcagcattg
    47881 gcttgaggaa gtttttggtc accgccggtg taatgcgatc cacccagcgt tcgatatatg
    47941 acaggcacca ggtcatcacc agcgccggga tcactgtgta ggtgtatttc accgcggtga
    48001 ccggaatcag ggcaaattcg acatgttcac cctgggccgc tttcgccatc agttcaataa
    48061 aactcggatg taccagcaca cccgcgatgg caatcgccag cgacatattg gttttaaatt
    48121 tgatggcggc agatgccgcg accatcagcg gcaggaagaa gaaagcaccg tcaccaatca
    48181 cattcagaat ggttaaggtc ggcgatcctt ttgtcagcac gccgctcatc tcgaggatca
    48241 tcgccagcag tttgaccatc gatccgccga taatcgccgg gatcagcggt gacatggtgc
    48301 cgatcagcgc atcgaggatc cctgcaccaa tgcgacgcag cgtgagtttg ggtttaccca
    48361 cgggctgtgc gggctgcata tctcccggca gcaggctgac gacttcctga aaggcttgtg
    48421 aaacggtatt gccgataatc acctgacact ggttgtcact acgtaccacg ccgagcacgc
    48481 cggggatggt ttttaacgtc gggctgtcga taagctggtc atctttgata acaaagcgca
    48541 aacgcgtcat acagtgcgtg accgccgaga tgttatcaac gccgcccagt gccgctatca
    48601 ccgagcgtgc cagcgccgca taatttttgg ccatcggatt ttatcctgtt atcagtaggg
    48661 tagatgtagt tgttatcagt cgacgtaacg gaaaaatgcc gatgctgaaa cggaattagg
    48721 aaaccggttt cacaaaatca tgaatagttg tcactaaata agcaagagag ttaattgttc
    48781 attttttata tttgtgaaac cggtcacctg aatatttttc ccgccgattg cgcaaggaca
    48841 atcggcaaac ggtgcagtac actgcgttga ttgcgtgtaa agggatgaaa atgatgacga
    48901 cgatgctgga agtggcgaag cgcgccgggg tttcaaaagc gaccgtttcc cgcgtgcttt
    48961 caggtaatgg ctacgtcagc caggagacta aagatcgcgt gtttcaggcg gtagaagaga
    49021 gcggttaccg cccaaatttg ctggcccgca atctgtcggc gaagagtact cagacgctgg
    49081 ggctggtagt gaccaacacg ctttatcacg gcatttattt tagtgaatta ctgtttcatg
    49141 ccgcgcgaat ggcggaagag aaagggcggc agttgctatt ggcagatggt aaacacagcg
    49201 ccgaagaaga gcgccaggcg attcagtatc tgctggattt gcgctgcgac gccattatga
    49261 tttacccgcg ttttttaagc gtggatgaga ttgatgacat cattgacgcg cacagtcagc
    49321 cgataatggt gcttaatcgc cgcctgcgca aaaacagcag ccatagcgtc tggtgcgatc
    49381 ataaacagac cagctttaat gccgtggcag agttgataaa cgccggacat caggagattg
    49441 ctttccttac cggctcgatg gattccccca ccagcgttga acgtcttgcc gggtataaag
    49501 acgcgctgtc gcagcatggt attgcgctca atgaaaaact tatcgctaac ggtaaatgga
    49561 cgcccgccag cggggccgaa ggggtagaaa cgttgctcga acgtggggct aaatttagcg
    49621 cgttagttgc cagtaacgac gatatggcga taggtgcgat caaagcgttg cacgagcgcg
    49681 gcgtagcggt gccagagcag gtgtcggtta tcggattcga tgatatcgca attgccccct
    49741 acaccgttcc ggcgctctcc agcgtaaaaa ttccggtaac agagatgatt caggaaatta
    49801 ttggacggct gatttttatg ctcgatggcg gggatttctc accgccgaaa accttcagcg
    49861 gaaaactgat ccgccgcgac tcactcattg ccctttcgcg ataaacattg tcatcacatg
    49921 tcatcgtcac tagtgtcgat gacatctttt ttcctgcatt acagaaatta atttccttaa
    49981 ataacagcaa attaaaaact ggcatgattt gtgaatgtat cggcgcatta actgtcattg
    50041 ctggagaatt tgatgaaccg tttcatcatt gctgacgcga gtaaatgtat tggttgccgt
    50101 acctgtgaag tggcgtgcgt ggtttctcat caggaaaatc aggactgtgc atcgctgacc
    50161 ccggaaacct ttttaccgcg aatccatgtc attaaaggtg tgaacatttc cacggcgaca
    50221 gtctgccgtc agtgtgaaga tgcaccgtgc gctaacgtct gcccgaatgg tgctatcagc
    50281 cgtgataaag ggtttgttca tgtgatgcag gaacgttgca ttggttgcaa aacctgcgtg
    50341 gtggcttgcc cgtatggcgc gatggaagtg gtggtacgtc cggtgatccg caacagcggc
    50401 gcggggctga atgtacgggc tgacaaagct gaagccaata aatgcgacct gtgcaaccat
    50461 cgcgaagacg gcccggcgtg tatggcggct tgcccgaccc atgcgctgat ttgtgtcgat
    50521 cgtaataaac ttgaacaact gagcgcagaa aaacgtcgcc gtacggcgct gatgttctaa
    50581 aaatcttata gaactgttct ggcatcaaag gaattcccgg aaatattcgt agaatacgga
    50641 gctggcggta tcgccgacct gagactgccg gatacgacgc agtcgtgtcc ggcagggcgg
    50701 gttacgcaat tcatgatggc gggaataact caatggcaaa aaacacatct tgcggtgtcc
    50761 aactgcgtat tcgaggcaaa gtgcagggcg tcggttttcg tccgtttgtc tggcagctgg
    50821 cacagcaatt aaatcttcac ggcgatgtct gtaatgacgg cgatggcgta gaagtccggc
    50881 tgctggaaga cccggaaacg tttcttgttc aattgcatca gcactgcccg ccgctggcgc
    50941 gtattgatag cgtcgagcgt gagccgtaca tctggtcaca actgcccacc gagttcacta
    51001 tacgccagag cacaggcggc accatgaata cgcaaattgt tcccgatgcc gctacttgcc
    51061 ctgcctgtct tgccgaaatg aataccccag gcgaacggcg ttatcgttat ccgtttatca
    51121 actgtaccca ctgcggcccg cgtttcacca ttattcgcgc catgccttac gaccgcccgt
    51181 ttaccgtgat ggcggcgttt ccgttgtgtc cggcttgtga caaagagtac cgcgacccgc
    51241 tcgatcgtcg cttccacgcc cagccggtgg cctgcccgga gtgtggccca catcttgaat
    51301 gggtaagtca tggtgaacat gcagaacaag aggcggcatt acaggcggct atcgcacagt
    51361 taaaaatggg caacattgtc gccatcaaag ggattggcgg atttcatctt gcctgcgatg
    51421 cacgtaacag taacgcggtg gcgacacttc gggcgcgcaa acatcgcccg gcgaaaccgc
    51481 tggcggtcat gttgccagtg gctgacggtt taccagacgc tgcgcgccag ttgcttacca
    51541 cgcccgccgc gccgattgtg ctggcggata aaaaatacgt tcctgagctt tgtgatgata
    51601 tcgcccctga ccttaacgaa gtcggggtaa tgttgcctgc gaacccgctc cagcatttgc
    51661 tgttacagga actgcaatgc ccgctggtga tgacctccgg caacctgagc ggtaaaccac
    51721 cagctatcag caacgaacag gcgctggcgg atttgcaggg cattgccgac ggattcttaa
    51781 tacataaccg cgacatcgtg cagcggatgg atgattcagt ggtgcgcgaa agcggcgaaa
    51841 tgctgcgccg ttcgcggggg tatgtgccgg atgcgctggc tttgcctccg ggctttaaaa
    51901 atgttccgcc tgtgctgtgt ctcggcgcgg atctgaaaaa taccttctgc ctggtgcgcg
    51961 gtgagcaagc ggtgttgagt cagcatctgg gcgatttaag tgacgatggc atccagatgc
    52021 agtggcgcga agcgttacgc ctgatgcaaa acatctacga ttttaccccg caatacgttg
    52081 tgcatgacgc gcatccgggc tatgtctcca gccagtgggc gcgcgaaatg aatctgccga
    52141 cgcaaacggt gctgcatcat catgcccacg cagcggcgtg tctggcagag catcagtggc
    52201 cgctggatgg cggtgatgtc attgctttga cgctcgacgg tatcggtatg ggggagaacg
    52261 gcgctttgtg gggcggcgag tgcctgcggg tgaactatcg cgaatgccag cacctgggcg
    52321 gcttgcctgc agtggcgctt ccgggtggcg atttggcggc gaagcagccg tggcgaaacc
    52381 tgctggcgca gtgcctgcgc tttgtgccgg agtggcagaa ttaccctgaa acagcaagtg
    52441 tgcaacagca aaactggagc gtgctggcgc gggccattga gcgtggaatt aacgcgccgc
    52501 tggcgtcatc gtgtgggcgg ttgttcgatg cagtggcggc ggcactgggc tgtgcgccag
    52561 ccacgttaag ttatgaaggt gaagcggctt gtgctctgga ggcgctcgca gcctcatgcc
    52621 acggagtgac gcatccggtg acgatgccgc tggtggacaa tcaactggat ctcgccgctt
    52681 tctggcagca gtggctgaac tggcaggcac cggttaatca acgcgcgtgg gcgtttcatg
    52741 atgcgctggc gcagggtttt gccgcgttga tgcgtgagca ggccacgatg cgtggtatca
    52801 ctacgctggt atttagcggc ggggttattc ataaccgttt gctgcgtgca cgtctggcgc
    52861 attatctcgc tgatttcaca ttgctctttc cacagagttt accggcgggt gatggcggtt
    52921 tgtctctggg gcagggggtt attgctgcgg cgcgttggtt agcgggtgaa gtccagaacg
    52981 gataaaacgt gttgcatttg gacaccattg ccggatgcgg cgtaaacgcc ttatccggcc
    53041 tacacatgcg tccccgtagg tcggataaga cgcgccagcg tcgcatccga cattttaggc
    53101 acagtagccc acctacatcg gcaatgtttt caacaatcca aatgcctctt tcatccgatc
    53161 ctcactgacc acaaaggcgc gaagctggtc agcatcgtca acaccgcgag ccaccattcc
    53221 ctggcgttcg gtattaattt gccagcgtaa atcctgacgc tgggtttcgc ctgccagatg
    53281 cagcggtaat tccggcgttt tgattttcac aagcatcgcc ggtagtttca gcggcgtgtt
    53341 attgccgaga agattttttg ccagcaccat cgcgctaagt tgaatcggct gcaggaatgg
    53401 cagtacctga ccgttgattt ccgcgcaatc gcccagcgca taaatatcgt cattactggt
    53461 ttgcagataa ctatcgacgc aaacgccgcg attaatcgtc agcccggcgc gtcgtgccag
    53521 ggcggtttcc gggcgcagtc cggtggcggc aattaccgca tccacttcga tgctacgctg
    53581 gcggtccagc gttgccagaa tgccagaatc cgttttttcc agcccctgta actgagattt
    53641 caacagcaga tgaacgccca tctccgtcaa ccgatgctgc aagcggctgc ttacttccgg
    53701 tggcattaac gacgccagaa tactggcagc gttgtcgatt agcgtgaccg ctttgcctgc
    53761 acgacaaaaa tccatcgcca gttcgctacc aatcaaacca ccgccaacaa tcaacacgcg
    53821 tcgggcatcc cgcagttgcg tttcacaggc gcgatactct tgctgactat ttaacgtcag
    53881 cattaactca cgcccaggca caggcgggac aaaggcactg gcaccggttg ccagtactag
    53941 cttgtcgtat tgccactgat tattctggct tttcaccaca cgggcttcgg catcgatatc
    54001 cgtcacccag gtttgtggaa acaggtgcag attaaactgc tcggcaaatt cacccgccgt
    54061 ctggcgggta aggtcatcgg cacgttgccc ctgactgata acatggctga ggtcaggttt
    54121 gttgtactca tccatgctgt cggcggcaat cagggttaat ggaatagtgg cgtcctgttt
    54181 gcgaatattt ttcaccagtt ggcgggcggc gaagcccgaa ccgatgatca caatgccgtt
    54241 actcattttg cctccgatgc cagttcgtca aagacgtctt tgccgaggga gcattccggg
    54301 cagaggaagt tatccgggac ttcactccac ggcgttcctg gcgcaacgtc ctgcattggc
    54361 tcgccttttg ccggatcgta aatccactgg cagacgctgc actgcattcg tgggccgagg
    54421 tcagccgtcg tcgtggcaga ggtttcttct ttaactaccg tattcaccgt gctctgcggc
    54481 agcggcgcga gcgcccactg acgggcgatt tcgcgaccgt gttcacggca taactccaga
    54541 gcgtcctggt ctggtcgcca tttggctttc aggctgagcg acatttcgaa acccgcatcc
    54601 tgcaggcgcg tggaaaggcg atccaccgca ccgccgctcc agccgtgaga gccgaaagca
    54661 ctggcgcgtt tgttacggaa gcgtaaacca gtcatctcct ccaccagccc ggcgattttc
    54721 ggcatcatca cgttattcat cgtcgaagtg ccgaccagca cgccttttga gcggaagaca
    54781 ttagtcagga tttcgttttt atcgcttcgg gcgacgttga aaattttcac cgccacgcgt
    54841 gggtcggttt ccgcaatccc ctgggcgata gcgtcagcca tcatgcgggt gttattcgac
    54901 atggtgtcgt agaaaatggt gattctgtct tcctgataat cagccgccca tttcaggtac
    54961 agctcgacaa tttgcgtcgg gttatcgcgc cataccacgc cgtgggaagt ggctatcata
    55021 tcgactggta agttaaagcc caggatctcg gtaattttcg gcgttaccag gcggctgaac
    55081 ggcgtcagga tattggcgta gtaacgctgg cactgctcga aaagctccgt ctgatccact
    55141 tcatcgttga acagatgctc gtcgcagtag tgctgaccga aagcatcgtt actgaacagc
    55201 accgcgtcgc ctgtcaggta agtcatcatg ctgtccggcc agtgcagcat tggtgtttcg
    55261 acaaaaatga gctgtttgcc gttgccgata tccagcgtgt cgccagtttt caccacatta
    55321 aaattccact ccggatgatg gtgatgacca tttatcgagt cgatagcgtt ggcagtacag
    55381 taaatcggcg tatcgggaat ttgcgccatc agttcggtca gcgccccggc gtggtcctct
    55441 tctgcatggt taatcacgat gtaatcgata tccgccagat cgatttcatt acgcaggttc
    55501 tgcacaaatt cgcggctgaa tttatggtcg acggtgtcga tcagcacgtt tttttcttcg
    55561 cggatgaggt agctgttgta gctgctgccg cgcagcgttt tatattccgt gccgtgaaaa
    55621 tcacgcactt cccagtcacg ttgacccacc caatgaatgt tatttttcac cacaatagac
    55681 atagcaacct caatttattc agcgtgttct aaaaagatgt cttgctaatt gcagattgcg
    55741 tgccagtttt ttatctgatt gtttttactc tataaataaa attatgctca ttaatgagta
    55801 gtcaaaatga ctatcaatca aatggtcaat atgacaatat ctatagtcaa attgacagtg
    55861 aggcaaagat gagtttttcc gttgatgtgc tggcgaatat cgccatcgaa ttgcagcgtg
    55921 ggattggtca tcaggatcgt tttcagcgcc tgatcaccac gctacgtcag gtgctggagt
    55981 gcgatgcgtc tgcgttgcta cgttacgatt cgcggcagtt tattccgctt gccatcgacg
    56041 gtctggcaaa ggatgtactc ggtagacgct ttgcgctgga agggcatcca cggctggaag
    56101 cgattgcccg cgccggggat gtggtgcgct ttcccgcaga cagcgaattg cccgatccct
    56161 atgacggttt gattcctggg caggagagtc tgaaggttca cgcctgcgtt ggtctgccat
    56221 tgtttgccgg gcaaaacctg atcggcgcac tgacgctcga cgggatgcag cccgatcagt
    56281 tcgatgtttt cagcgacgaa gagctacggc tgattgctgc gctggcggcg ggagcgttaa
    56341 gcaatgcgtt gctgattgaa caactggaaa gccagaatat gatgccaggc gatgccacgc
    56401 cgtttgaagc ggtgaaacag acgcagatga ttggcttgtc ccctggcatg acgcaactga
    56461 aaaaagagat tgagattgtg gcggcgtccg atctcaacgt cctgatcagc ggtgagacgg
    56521 gaaccggtaa ggagctggtg gcgaaagcga ttcatgaagc ctcgccacgg gcggtgaatc
    56581 cgctggtcta tctcaactgt gccgcactgc cggaaagtgt ggcggaaagt gaactgttcg
    56641 ggcatgtgaa aggagcgttt actggcgcta tcagtaaccg cagcgggaag tttgaaatgg
    56701 cggataacgg cactctgttt ctggatgaga tcggcgagtt gtcgttggca ttgcaggcca
    56761 agctgctgag ggtgttgcag tatggcgata ttcagcgcgt tggcgatgac cgcagtttgc
    56821 gggtcgatgt gcgcgtgctg gcggcgacta accgcgactt acgcgaagag gtgctggcag
    56881 ggcgatttcg cgctgacttg tttcatcgcc tgagcgtgtt tccactttcg gtgccgccgc
    56941 tgcgtgagcg gggcgatgat gtcattctgc tggcggggta tttctgcgag cagtgtcgtt
    57001 tgcggctggg gctctcccgc gtggtattaa gtgccggagc gcgaaattta ctgcaacact
    57061 atcgttttcc ggggaacgtg cgcgaactgg aacatgctat tcatcgggcg gtagtgctgt
    57121 cgagagccac ccgcaacggc gatgaagtga ttcttgaggc gcaacatttt gcttttcctg
    57181 aggtgacgtt gccgacgcca gaagtggcgg cggtgcccgt tgttaagcaa aacctgcgtg
    57241 aagcgacaga agcgttccag cgtgaaacta ttcgtcaggc gctggcacaa aatcatcaca
    57301 actgggctgc ctgcgcgcgg atgctggaaa ccgacgtcgc caacctgcat cggctggcga
    57361 aacgtctggg aatgaaggat taaataatcc cggcctgata gaaatcctgc aggttaattg
    57421 cgccggtgag tttgccgttt tcatctacca ccggtgcggc agtgattttg cgcttcatca
    57481 ggatctcttt ggcgtcgatg gcgcgacttt gcgattgcaa cgtggtgccg ccgaccgtca
    57541 tcgcttcatt aactggcgtg gtgagtgcgc cgccgccaac cagccagcga cgtaaatcgc
    57601 cgtcggtaaa gacgccttgt acctgttgtt gagcgtcaca taccgccacc agccccagac
    57661 cggtgcggct gagttccagc atcgcatcca tcacgctggc ggttaacgcc acctgtggga
    57721 tggcatcgtc acggcgcatc agatgatgca ctttattcag caagcgagcg cccagtgccc
    57781 cggctgggtg ggagcgggca aaatcttctt cattaaatcc gcgcgcctgc atgaccgcca
    57841 tcgccagcgc gtcacccatc atcagggtat tgacggtgct ggaggtcggc gcaaggtgca
    57901 tcgggcaggc ttcgcgttct acggagatat ccagcaccgc tttcgccgcc aggcccagcg
    57961 gtgacgtcgg tttgccggtc atcgccagca gcgcgataga tttatcttcc agacgcggaa
    58021 taatcagatc cagttccttc gcgccaccgg agtaagagat aaacagcatc acatcgcggc
    58081 tttcaatcat ccccagatcg ccgtgcagcg cttctgccgg atggacaaaa aaagccggtg
    58141 tgccggtact ggcaagtgtt gcggcgattt tcttaccaat gtggcccgat ttgccaattc
    58201 ccgaaaccac cactttgcct tcgcagtgca ggatgatatt ggcggcgcga acaaaatcat
    58261 cgcccagacg ttccggtaaa cggcttgctt cctgcaactc cagcattaac gtctgacgtc
    58321 ccgcgttcag tagtgcttca ctcattgctc tctccggtta tgatcacatc gatccctttc
    58381 tcttccagcg cctgacgaaa cgccggatcg atacctgcgt cggtaatcag cttatcgacg
    58441 ctttcaagac tgcaaactac gttggggctt ttacggccaa actttgatga gtccgccatc
    58501 aaaatcactt cgcgcgcggc attgcacatt gccttactga cggtataaac ctcgttgaag
    58561 gtggttacgc ccgcattgag atcgatgccg tcggtgccca taaacaattt atcgaaactg
    58621 aaatgttcga aggcattctc tgccagctgt ccgtgaaatg aggccgattt tttacgaaac
    58681 gttccgcctg gcatcaggat agtttgttcg ttatccagtt cggatagcgc attgacgata
    58741 tgcaggctgt tggtcatcac cgtgatgtta ttaaagcgcg agagcagggg aaccatctgc
    58801 aaaacggtac tgccagcatc aagaatgatc gaatcgccat catggataaa actaacggca
    58861 gcttctgcaa tcagctcttt cttgtgggtg ttgatgagcg ttttatgatc gataggcgga
    58921 tcggattcct ctttattcaa caccactccg ccataagtac gaatgacggt tccggcatgt
    58981 tccagaatga ccagatcttt gcgaatggtt gtgcctgtgg tgtcaaagta ttgcgccaat
    59041 tcttcaaccg agcatttacc ctgcttttgc agatactcca gaatggcggc ctgacgctga
    59101 cgaggtttca tgggcgtgat tacctgattt aggtttcaaa atgataactt cgcaagcaag
    59161 taacattcac aacgaaatta tccacgtttc agtttaagcg ccaatgatag agcattctgt
    59221 gatagtggat catggggaaa gatcacatcg ggctgtaaat cacccgcatg cataccggta
    59281 attgcgggaa ttttttgcgg tcgggcgaag acggtcagtc ctttcataaa caaggtgtcg
    59341 acgatgcgct gctgatcgtc cagcgcgatg gcgaccacga cccgcggttt aaagcgcccg
    59401 ctggaacggc ccacgccaac ccgcccttgc tggcatagtg tgtcgaaggc acggttaaaa
    59461 cgagaaattt gccagccgcc taaggccagt tgcgcacacc aggcgataac ggcgacggtg
    59521 atgagtgcgg ataccataac ttttccttaa tgtagtggcg gaagtcctgt atgcctgatg
    59581 agcgtagcgc atcaggcatt ctatagtctc acggagatcc ttaacggatc tccgctgtta
    59641 atcagaacat cacctgaccg ccggtgacat tgatcgactg tccggtgcag tacgacgcct
    59701 taggactggc gtagaacagc agcatattca gcacgtcctg ataatcgcag ccgcgtttga
    59761 gcggtacttt gtcgatgtaa tactgctcga cttgatccgg tttgataccc agcttggtcg
    59821 cgtattgtgg caacagtgac tggaacatcg gcgatttcag caggttaccg agcatcagtg
    59881 aatgcaccgt aatgccgtac tccgccagat ccagcgccag tgattgagtc agcccgacgc
    59941 caccaaattt cgctgcgctg tagccagagt tgtgtttgct gcccactttg ccggatttcg
    60001 agttgatctg aataatgcga ccctgaatcc cgtcgcggat catcaaacgc gaaaattcac
    60061 gggcacacag gaaataaccc accagattca cttgtagcga acggtcaaaa tcgccgagct
    60121 ggaagtcgct gataaaggct gcttttgcta ttccggcgct atagaccagc aaatccacgc
    60181 gaccaaagat ttcatctacc ccacgagaga gcgccagaac gctttgctcg ctagtggcgt
    60241 cagcaccaaa accgtacgcc atactttcac catattcggc gttaatttct tgtgccacat
    60301 ttgcggcttt gtcgctctga atatcgacaa ccgcgacgcg atacccctcg gcagccagac
    60361 cgtggcacag gaacgcgcct aatgtttgcc caccaccgat gacaacggca acctgattca
    60421 ttttttactc cttaacagat tcaaacttca aaaccgatcc cggcgcgata tcgtcgggta
    60481 cagggcctgc cacatggaca gtgcccggaa attccgcttc gtttaaacca tcgaagcgca
    60541 gggtgacatg acccagttcg cgaaggttgt cttccgccac gctgccaacg gcggtcaccg
    60601 gatagcgatg ctgcccgagc gtaaattgca aaccgggatg gagtgcacct ttcaactcgc
    60661 cgtggcaatg aatgaagcaa tactcttcga ggtccgctgg cgcgccttca cgaaaggtga
    60721 tgagcatctg gtcgctgagg gcgtcagtgg cactcgcgcc gatacgggtg atggtggtct
    60781 gataaataac ggtcatgttt cagcctctat tgatagataa aaccggagac aaaccaggcg
    60841 atcagtacag tcggtgcgcc ggttaaaaag cggctcacca gtacagaagg gacaccgacg
    60901 cgaaccgtgt cctgacgggc ttccgccagc gacaaaccga ccgggatgaa gtcgcaggcc
    60961 gcctgcgcgt tgatggcaaa cagtgccggt aaagccagat gcggcggaat attgccgaga
    61021 ccaatctgca cgccaatcaa tacgccgata acctgtgcga taactgcgcc tgggccgagg
    61081 aaaggtgaaa gcagtgggaa ggagcagatg agcgccagca tgaccagacc cagtggatgg
    61141 ctcgccagcg gagcaagacc gtgggcaatc cagtcaccaa ggccagaagc cataatgatg
    61201 ccaatgagcg ccgagacgaa tgccataaac ggcagaatgg tttttaatac agtgtcgatg
    61261 gtgtcacgac cagattgaaa caacacggca acggcagaac ccatccccat tcccaccttc
    61321 gccagtaaac catcgctttg ttcggtgatt ttcttgctgg tgtcatagtc acggcccacg
    61381 gaagagggtt gtggtgtcgc atcacccact acagtgatgt tttcttcttt tacgccagag
    61441 acataaatat cttccacaat gtactgcgcc agtggaccgg acttgcccgt cgagtggata
    61501 ttaatggtgg gaatacgccg tttcggatag atgccgcagc gtaatgtgcc gccacagtcg
    61561 attaccgcga caccaatttc cgcctccgcg ggttcacctt ctttaaatcc gtcaatagcc
    61621 tgccagccag taagttgtgc cagtttgtca acaatcgcag gccgggtacc ggcggtgata
    61681 tagacgattt ttttgcccgg cgtggcttcc agctcaagcg ggccgcccca gccacccgtt
    61741 cctttttcga tccgaatacg cgtcatgatg ttgctcctgc caggtgaact ttttgttcaa
    61801 gttgaatgcc catctttttc tcaaaaacgg cggtggtcag atcggtcacc cagccgcgga
    61861 agaaattggt gaccagacca accagcagat aactcaccgc cagtgggcca agtggcaggt
    61921 tcagcgttgt cagaccgctg gcaatgccaa gataaacaaa cagttcgcca gggttgatat
    61981 gggggaagag gccattcatt gagtggcagc tataagaggc cgccgcgtag tagctgggtt
    62041 tgtacttttc cggcataaag cgacccaggc ttaaggtcat cggattgcaa aagacaaacg
    62101 tgccaatgca cggtaacagt aggtaacggg aaacagggtt accggcgcaa cgttgagcaa
    62161 aacgttcaat acgatgctga ccgataaaat taatcagtgc gttcatgata accagcaggc
    62221 taatcaacag cggaagaatg ccggtcacca tcccggtaaa cacctctccg cccttttgga
    62281 acagcccgat aaaccactct gcaccatgag taatggtttc tatcattgtt ctctccttca
    62341 ggatttattg ttttattacc aaacggcaac ctaatctaat cagattgaaa gatttaaaag
    62401 tgttattttg atctcaaaat gaaagataaa tattttaatt tgaaagtttg agtaaaagga
    62461 tagcgagggg aatgagttga gttatgtaaa gtccgtatcg ggcagtgact accgcttcct
    62521 tgtgtggggc gtgatgcttt accatacttg cccctggttg aatctgttaa atggacccct
    62581 catgttcaag cgtcgttatg taacattgct tccccttttt gtgttgcttg ccgcctgtag
    62641 cagcaagcca aaacctactg agactgaaac gaccaccgga acgccgtctg gcggcttcct
    62701 gcttgagccg cagcacaatg tgatgcagat gggcggcgat ttcgctaata acccgaatgc
    62761 ccagcagttc atcgacaaaa tggtgaacaa acacggtttc gatcgtcagc agttgcagga
    62821 aattctctcc caggcgaagc gtctggattc ggtactgcgg ctgatggata accaggcacc
    62881 aaccacatcg gtgaaaccgc catcaggtcc gaacggcgca tggctccgtt atcgcaaaaa
    62941 atttattacg ccggacaacg tgcagaacgg tgtggttttc tggaatcagt atgaagatgc
    63001 gttgaatcgc gcgtggcagg tgtatggcgt accgccggaa attatcgtcg ggattatcgg
    63061 cgttgaaacc cgctgggggc gcgtgatggg gaaaactcgc atcctcgatg cgctggcaac
    63121 gctgtcattt aactacccac gccgcgcgga gtatttctct ggcgagctgg aaaccttcct
    63181 gttgatggcg cgcgacgagc aggacgatcc gctcaatctg aaaggttcct ttgccggggc
    63241 gatgggctac ggacagttta tgccgtcgtc ttacaaacaa tatgcggtag atttcagcgg
    63301 cgacgggcat atcaacctgt gggatccggt tgatgcgatc ggtagcgtgg cgaactattt
    63361 caaagcgcac ggctgggtga aaggcgatca ggtcgcggta atggcaaacg gtcaggctcc
    63421 aggcttgcca aatggcttca aaaccaggta cagcatttcg cagcttgcca cggcaggttt
    63481 aacgccacag cagccgctgg gcaaccatca acaagccagc ctgctgcgtc tggatgtcgg
    63541 taccggttac cagtactggt acggtctgcc gaacttctac accattaccc gttataacca
    63601 cagcacccat tacgcaatgg cggtctggca gttaggacaa gccgtggcgc tggcgcgcgt
    63661 gcagtaacta tttgcaagag gggggcgtga gcttccctct ggctaatcag cttttttctg
    63721 aatcctcctc gtaaaattgc aacgccaaca ccatcttcct gacgaaagtg ctatcttgtc
    63781 cggcataaat ttttgactga cagaggttgt gatgactgac agtgaactga tgcagttaag
    63841 tgaacaggtt gggcaggcgc tgaaagcccg tggcgcaacc gtaacaactg ccgagtcttg
    63901 taccggtggt tgggtagcga aagtgattac cgatattgcc ggtagctccg cctggtttga
    63961 acgcggattt gtcacctaca gtaacgaagc caaagcgcag atgatcggcg tacgcgaaga
    64021 gacgctggcg cagcatggcg cggtgagtga acccgtcgtg gtggaaatgg cgataggtgc
    64081 actgaaagcg gctcgtgctg attatgccgt gtctattagt ggtatcgccg ggccggatgg
    64141 cggcagtgaa gagaagcctg tcggcaccgt ctggtttgct tttgccactg cccgcggtga
    64201 aggcattacc cggcgggaat gcttcagcgg tgaccgtgat gcggtgcgtc gtcaggctac
    64261 tgcgtatgca ttgcagacct tgtggcaaca atttctacaa aacacttgat actgtatgag
    64321 catacagtat aattgcttca acagaacata ttgactatcc ggtattaccc ggcatgacag
    64381 gagtaaaaat ggctatcgac gaaaacaaac agaaagcgtt ggcggcagca ctgggccaga
    64441 ttgagaaaca atttggtaaa ggctccatca tgcgcctggg tgaagaccgt tccatggatg
    64501 tggaaaccat ctctaccggt tcgctttcac tggatatcgc gcttggggca ggtggtctgc
    64561 cgatgggccg tatcgtcgaa atctacggac cggaatcttc cggtaaaacc acgctgacgc
    64621 tgcaggtgat cgccgcagcg cagcgtgaag gtaaaacctg tgcgtttatc gatgctgaac
    64681 acgcgctgga cccaatctac gcacgtaaac tgggcgtcga tatcgataac ctgctgtgct
    64741 cccagccgga caccggcgag caggcactgg aaatctgtga cgccctggcg cgttctggcg
    64801 cagtagacgt tatcgtcgtt gactccgtgg cggcactgac gccgaaagcg gaaatcgaag
    64861 gcgaaatcgg cgactctcac atgggccttg cggcacgtat gatgagccag gcgatgcgta
    64921 agctggcggg taacctgaag cagtccaaca cgctgctgat cttcatcaac cagatccgta
    64981 tgaaaattgg tgtgatgttc ggtaacccgg aaaccactac cggtggtaac gcgctgaaat
    65041 tctacgcctc tgttcgtctc gacatccgtc gtatcggcgc ggtgaaagag ggcgaaaacg
    65101 tggtgggtag cgaaacccgc gtgaaagtgg tgaagaacaa aatcgctgca ccgtttaaac
    65161 aggctgaatt tcagatcctc tacggcgaag gtatcaactt ctacggcgaa ctggttgacc
    65221 tgggcgtaaa agagaagctg atcgagaaag caggcgcgtg gtacagctac aaaggtgaga
    65281 agatcggtca gggtaaagcg aatgcaactg cctggctgaa agataacccg gaaaccgcga
    65341 aagagatcga gaagaaagta cgtgagttgc tgctgagcaa cccgaactca acgccggatt
    65401 tctctgtaga tgacagcgaa ggcgtagcag aaactaacga agatttttaa tcgttcgtta
    65461 ttgatgataa tagataatgt gtgcacggac ggtcccctcg cccctttggg gagagggtta
    65521 gggtgagggg aacaggccag cactggcgtg aaaaattact ctcacctcga tcctctaacg
    65581 aattgggcct gcgggaacat tctgcacact cattgactat taaatctgtt tatcagcaaa
    65641 acctgggtcg catctgcggc ccttttgctt ttttaagttg taaggatatg ccatgacaga
    65701 atcaacgtcc cgtcgcccgg catatgctcg cctgttggat cgtgcggtac gcattctggc
    65761 ggtgcgcgat cacagtgagc aagaactgcg acgtaaactc gtggcaccga ttatgggcaa
    65821 aaatggccca gaagagattg atgctacggc agaagattac gagcgcgtta ttgcctggtg
    65881 ccatgaacat ggctatctcg atgacagccg atttgttgcg cgctttatcg ccagccgtag
    65941 ccgcaaaggt tatggacctg cacgtattcg tcaggaactg aatcagaaag gtatttcccg
    66001 cgaagcgaca gaaaaagcga tgcgtgaatg tgacatcgac tggtgcgcac tggcgcgcga
    66061 tcaggcgacg cgaaaatatg gtgaaccttt gccaactgtc ttttcagaaa aagttaagat
    66121 ccagcgtttt ctgctctatc gtggctatct gatggaagat atccaggata tttggcgaaa
    66181 ttttgccgac tgaacgcata cggtatttta cttcccagtc aagaaaactt atcttattcc
    66241 cacttttcag ttaccagccc ggcggttaag acacgctgga gctggtggcg atatttcgtt
    66301 agcttgattt caggataatt atgagcaaga gcaccgctga gatccgtcag gcgtttctcg
    66361 actttttcca tagtaaggga catcaggtag ttgccagcag ctccctggta ccccataacg
    66421 acccaacttt gttgtttacc aacgccggga tgaaccagtt caaggatgtg ttccttgggc
    66481 tcgacaagcg taattattcc cgcgctacca cttcccaacg ctgcgtgcgt gcgggtggta
    66541 aacacaacga cctggaaaac gtcggttaca ccgcgcgtca ccataccttc ttcgaaatgc
    66601 tgggcaactt cagcttcggc gactatttca aacacgatgc cattcagttt gcatgggaac
    66661 tgctgaccag cgaaaaatgg tttgccctgc cgaaagagcg tctgtgggtt accgtctatg
    66721 aaagcgacga cgaagcctac gaaatctggg aaaaagaagt cgggatcccg cgcgaacgta
    66781 ttattcgcat cggcgataac aaaggtgcgc catacgcatc tgacaacttc tggcagatgg
    66841 gtgacactgg tccgtgcggc ccgtgcaccg aaatcttcta cgatcacggc gaccacattt
    66901 ggggtggccc tccgggaagt ccggaagaag acggcgaccg ctacattgag atctggaaca
    66961 tcgtcttcat gcagttcaac cgccaggcag atggcacgat ggaaccgctg ccgaagccgt
    67021 ctgtagatac cggtatgggt ctggagcgta ttgctgcggt gctgcaacac gttaactcta
    67081 actatgacat cgacctgttc cgcacgttga tccaggcggt agcgaaagtc actggcgcga
    67141 ccgatctgag caataaatcg ctgcgcgtaa tcgctgacca cattcgttct tgtgcgttcc
    67201 tgatcgcgga tggcgtaatg ccgtccaatg aaaaccgtgg ttatgtactg cgtcgtatca
    67261 ttcgtcgcgc agtgcgtcac ggcaatatgc tcggcgcgaa agaaaccttc ttctacaaac
    67321 tggttggtcc gctgatcgac gttatgggct ctgcgggtga agacctgaaa cgccagcagg
    67381 cgcaggttga gcaggtgctg aagactgaag aagagcagtt tgcccgtact ctggagcgcg
    67441 gtctggcgtt gctggatgaa gagctggcaa aactttctgg tgatacgctg gatggtgaaa
    67501 cggctttccg tctgtacgac acctatggct tcccggttga cctgacggct gatgtttgtc
    67561 gtgagcgcaa catcaaagtt gacgaagctg gttttgaagc agcaatggaa gagcagcgtc
    67621 gtcgcgcgcg cgaagccagc ggctttggtg ccgattacaa cgcaatgatc cgtgttgata
    67681 gtgcatctga atttaaaggc tatgaccatc tggaactgaa cggcaaagtg accgcgctgt
    67741 ttgttgatgg taaagcggtt gatgccatca atgcaggcca ggaagctgtg gtcgtgctgg
    67801 atcaaacacc attctatgcg gaatccggcg gtcaggttgg tgataaaggc gaactgaaag
    67861 gcgctaactt ctccttcgcg gtggaagata cgcagaaata cggccaggcg attggtcaca
    67921 tcggtaaact tgctgcgggt tctctgaaag tgggcgacgc tgtgcaggct gatgttgatg
    67981 aggctcgtcg cgcccgtatt cgtctgaatc actccgcaac gcacctgatg cacgctgcgc
    68041 tgcgccaggt tctggggact catgtatcgc agaaaggttc actggttaac gacaaggtgc
    68101 tgcgcttcga cttctcacac aacgaagcga tgaaaccgga agagattcgt gcggtcgaag
    68161 acctggtgaa cgcacagatt cgtcgcaatt tgccgatcga aaccaacatc atggatctcg
    68221 aagcggcgaa agcgaaaggt gcgatggcgc tgttcggcga gaagtatgat gagcgtgtac
    68281 gcgtgctgag catgggcgat ttctccaccg agctgtgtgg cggtactcac gccagccgca
    68341 ctggtgatat tggtctgttc cgcatcatct ctgaatcggg tactgctgca ggcgttcgtc
    68401 gtatcgaagc ggtaaccgga gaaggcgcta tcaccaccgt tcatgcagac agcgatcgct
    68461 taagcgaagt cgcgcatctg ctgaaaggcg atagcaataa tctggcggat aaagtgcgct
    68521 cagtactgga acgtacgcgt cagttagaaa aagaactaca acagcttaaa gaacaagctg
    68581 ccgcacagga gagcgcaaat ctttccagta aggcaattga tgttaatggt gttaagctgt
    68641 tggttagcga gcttagcggt gttgagccga aaatgttgcg taccatggtt gacgatttaa
    68701 aaaatcagct ggggtcgaca attatcgtgc tggcaacggt agccgaaggt aaggtttctc
    68761 tgattgcagg cgtatctaag gacgtcacag atcgtgtgaa agcaggggaa ctgattggta
    68821 tggtcgctca gcaggtgggc ggcaagggtg gtggacgtcc tgacatggcg caagccggtg
    68881 gtacggatgc tgcggcctta cctgcagcgt tagccagtgt gaaaggctgg gtcagcgcga
    68941 aattgcaata atataagcgt caggcaatgc cgtggactcg cttcacggca ttcgcattaa
    69001 cgctatcgac aacgataaag tcaggttgaa gttgtgtata tcggctaaac ttaggtttaa
    69061 cagaatgtaa tgccatgact gcttacatgt aatgtgtttg tcattgctta ctttttggcg
    69121 ttatatgatg gataatgccg ggatacagag agacccgact cttttaatct ttcaaggagc
    69181 aaagaatgct gattctgact cgtcgagttg gtgagaccct catgattggg gatgaggtca
    69241 ccgtgacagt tttaggggta aagggcaacc aggtacgtat tggcgtaaat gccccgaagg
    69301 aagtttctgt tcaccgtgaa gagatctacc agcgtatcca ggctgaaaaa tcccagcagt
    69361 ccagttacta atctttccgc gtctcatctt tatcggtgag acgcaccctc aaaatttctc
    69421 cttcactcta tagtcttttc gctttactcc cgttcattca acttaagtct ccattttttt
    69481 gcattactac tatctgtcag acctccattt ttctgttgat aaaacactct ttttgacgtt
    69541 tttacagact aattgaacgt gaagtgtgca aacgataaaa gtgtaggaaa aattgtttga
    69601 cttataagtc tcagaaagta atatgtgcgc cacgcagcga cgatgagcga taaacaagtt
    69661 cttcgaagca ctcgtaagag gcgtgtggtg aggtggccga gaggctgaag gcgctcccct
    69721 gctaagggag tatgcggtca aaagctgcat ccggggttcg aatccccgcc tcaccgccat
    69781 ttgcatccgt agctcagctg gatagagtac tcggctacga accgagcggt cggaggttcg
    69841 aatcctcccg gatgcaccat attctacgta ctttcagcga tgaaggtatg gaagatgtgg
    69901 cggtataacc gcaggcacca gggaggataa cgttgcttta gcaacggccc gaagggcgag
    69961 ccgcaaggcg agtaatcctc ccggatgcac catctcttac ttgatacggc tttagtagcg
    70021 gtatcaaaaa atctgcagta aagtaagttt cccgatgcat ccgtagctca gctggataga
    70081 gtactcggct acgaaccgag cggtcggagg ttcgaatcct cccggatgca ccatctctta
    70141 cttgatatgg ctttagtagc ggtatcaata tcagcagtaa aataaatttt cccgatgcat
    70201 ccgtagctca gctggataga gtactcggct acgaaccgag cggtcggagg ttcgaatcct
    70261 cccggatgca ccatattctc cgtaaccttc agcaatgaag gtatcgaaga gatagcggta
    70321 ttaaccgcag gcaccaggga ggataacgtt gcgtcagcaa cggcccgaag ggcgaacgcc
    70381 agtgagtaat cctcccggat gcaccatctt ctcccaaaaa tttccttcaa aaatatctca
    70441 tttctaaata actactttat ccatttcctg aattttcttc tccttcgcca tcagcgacaa
    70501 aatcaagcaa ttacgctaaa gtagcgtcat atttttcgac ttgcgagaag accatgtacg
    70561 agcgttatgc aggtttaatt tttgatatgg atggcacaat cctggatacg gagcctacgc
    70621 accgtaaagc gtggcgcgaa gtattagggc actacggtct tcagtacgat gttcaggcaa
    70681 tgattgcgct taatggatcg cccacctggc gtattgctca ggcaattatt gagctgaatc
    70741 aggccgatct cgacctgcat gcgttagcgc gtgaaaaaac agaagcagta agaagtatgc
    70801 tgctggatag tgtcgagccg cttcctcttg ttgaagtggt gaaaagttgg catggtcgtc
    70861 gcccaatggc tgtaggaacg gggagtgaaa gcgccatcgc tgaggcattg ctggcgcacc
    70921 tgggattacg ccgttatttt gacgccgtcg tcgctgctga tcacgtcaaa caccataaac
    70981 ccgcgccaga cacatttttg ttgtgcgcgc agcgtatggg cgtgcaaccg acgcagtgtg
    71041 tggtctttga agatgccgat ttcggtattc aggcggcccg tgcagcaggc atggacgccg
    71101 tggatgttcg cttgctgtga gtgaagcgtt atcgcttttc tcgttgtttg ccagtagttt
    71161 tctcagcgct acactattac ccggcaactc cgaagtcgtg ctggtggcaa tgttgctttc
    71221 cgggatcagt tatccctggg ttttagtttt aacagcaaca atgggtaata gccttggagg
    71281 gttaactaac gttatccttg ggcgtttctt tccattgcgt aaaacatcgc gctggcaaga
    71341 gaaagctacc ggctggctga aacgctatgg tgcagtcacg ctattattaa gctggatgcc
    71401 cgtggttggc gatttactgt gtctgttagc gggatggatg cgcatctcct gggggccagt
    71461 aatctttttt ttgtgccttg gtaaagcgtt acgctatgtt gcagttgcag cagcgaccgt
    71521 tcagggcatg atgtggtggc actaattgta ggcctgcaca tatggtcacc attacagtta
    71581 tgctaattaa aacgattttg acaggcggga ggtcaatttg atcccggacg tatcacaggc
    71641 gctggcctgg ctggaaaaac atcctcaggc gttaaagggg atacagcgtg ggctggagcg
    71701 cgaaactttg cgtgttaatg ctgatggcac actggcaaca acaggtcatc ctgaagcatt
    71761 aggttccgca ctgacgcaca aatggattac taccgatttt gcggaagcat tgctggaatt
    71821 cattacacca gtggatggtg atattgaaca tatgctgacc tttatgcgcg atctgcatcg
    71881 ttatacggcg cgcaatatgg gtgatgagcg gatgtggccg ttaagtatgc catgctacat
    71941 cgcagaaggt caggacatcg aactggcaca gtacggcact tctaacaccg gacgctttaa
    72001 aacgctgtat cgtgaagggc tgaaaaatcg ctacggcgcg ctgatgcaaa ccatttccgg
    72061 cgtgcactac aatttctctt tgccaatggc attctggcaa gcgaagtgcg gtgatatctc
    72121 gggcgctgat gccaaagaga aaatttctgc gggctatttc cgcgttatcc gcaattacta
    72181 tcgtttcggt tgggtcattc cttatctgtt tggtgcatct ccggcgattt gttcttcttt
    72241 cctgcaagga aaaccaacgt cgctgccgtt tgagaaaacc gagtgcggta tgtattacct
    72301 gccgtatgcg acctctcttc gtttgagcga tctcggctat accaataaat cgcaaagcaa
    72361 tcttggtatt accttcaacg atctttacga gtacgtagcg ggccttaaac aggcaatcaa
    72421 aacgccatcg gaagagtacg cgaagattgg tattgagaaa gacggtaaga ggctgcaaat
    72481 caatagcaac gtgctgcaga ttgaaaacga actgtatgcg ccgattcgtc caaaacgcgt
    72541 tacccgcagc ggcgagtcac cttctgatgc gctgttacgt ggcggcattg aatatattga
    72601 agtgcgttcg ctggacatca acccgttctc gccgattggt gtagatgaac agcaggtgcg
    72661 attcctcgac ctgtttatgg tctggtgtgc gctggctgat gcaccggaaa tgagcagtag
    72721 cgaacttgcc tgtacgcgcg ttaactggaa ccgggtgatc cttgaaggtc gcaaaccggg
    72781 cctgacgctg ggtatcggct gcgaaaccgc gcagttcccg ttactgcagg tgggtaaaga
    72841 tctgttccgc gatctgaaac gcgtcgcgca aacgctggat agcattaacg gcggcgaagc
    72901 gtatcagaaa gtgtgtgatg aactggttgc ctgcttcgat aatcccgatc tgactttctc
    72961 tgcccgtatc ttaaggtcta tgattgatac tggtattggc ggaacaggca aagcgtttgc
    73021 agaagcctac cgtaatctgc tgcgtgaaga gccgctggaa attctgcgcg aagaggattt
    73081 tgtagccgag cgcgaggcat ctgaacgccg tcagcaggaa atggaaaccg ctgataccga
    73141 accgtttgcg gtgtggctgg aaaaacacgc ctgacagaaa agaaaaaggc cactcgtgag
    73201 tggccaaaat ttcatctctg aattcaggga tgatgataac aaatgcgcgt ctttcatata
    73261 ctcagactcg cctgggaaga aagagttcag aaaattttaa aaaaaattac cggaggtggc
    73321 taaatgccgt tgttagatag cttcacagtc gatcataccc ggatggaagc gcctgcagtt
    73381 cgggtggcga aaacaatgaa caccccgcat ggcgacgcaa tcaccgtgtt cgatctgcgc
    73441 ttctgcgtgc cgaacaaaga agtgatgcca gaaagaggga tccataccct ggagcacctg
    73501 tttgctggtt ttatgcgtaa ccatcttaac ggtaagggtg tagagattat cgatatctcg
    73561 ccaatgggct gccgcaccgg tttttatatg agtctgattg gtacgccaga tgagcagcgt
    73621 gttgctgatg cctggaaagc ggcaatggaa gacgtgctga aagtgcagga tcagaatcag
    73681 atcccggaac tgaatgtcta ccagtgtggc acttaccaga tgcactcgtt gcaggaagcg
    73741 caggatattg cgcgtagcat tctggaacgt gacgtacgca tcaacagcaa cgaagaactg
    73801 gcactgccga aagagaagtt gcaggaactg cacatctagt cagtaaacta tcttcacaat
    73861 taattgaaaa aagccagttc aaatgaactg gcttagttgt acttagtgcg caccgcctcc
    73921 gccgccacct gcgccaaatg gcggtttagc aaaccacacc agccccagca gtacgaggaa
    73981 tatcccggct gacatccaga agatctcatt ggcggaaata atcagcccct gattggtgat
    74041 ctgctgggca atccagcctg atgcctgctg ttgcgtcatc ccaagccctt ccagttgact
    74101 gtacatcgcc tgggcattcg ggttgaacgg gtttaccgac tcagtcaact gcgcatggtg
    74161 catcgactcg cggttggtcc acatggtcgt ggttatcgac gtgccgatag accccgccag
    74221 cgttcgcgta aagttagaga ggctcgatgc cgccgccagt cgttccggtg gcaaaccaga
    74281 cagcgtaatg gtggtcagcg gcataaagaa gcaggccacc gcaaacccct ggataaactg
    74341 cggccaggcc gacgcgccaa aatccatacc tggttcaaag gtataggcac gccagtagaa
    74401 gcagacggca tacataataa agctgaaggt taccagccgc cgcatatcca gtttatgcgc
    74461 gaagcggccg ataatcggcg acaggatcac cggaataatc cctaccggcg cagaggccaa
    74521 acctgcccag gtcgccgtgt aaccgtagac ctcctgcaac aactgcggca gcagaacaat
    74581 agcgccgaag tagagcatat acgcgaggct gatacacaag cagccgatgg tgaagttgcg
    74641 cgacttaaac aacgacagat cgactatcgg gttatcgtcg gtcagctccc agacaatcag
    74701 gaagcagata gccaccaccg ccaccacggt aagaatgata atttcctgtg atgaaaacca
    74761 gtccagctct ttaccgcggt cgagcataat ctgcaggctg ccgataccaa taaccagcag
    74821 tgccagcccc acggcatcaa tccgccgccg ttcggtgcgg gtttcacgtc cgcgcagagt
    74881 ttgcagtgtc atcaacacca ccgccacgcc aatcggcacg ttgatgaaga atatccagcc
    74941 ccagtggtaa ttatcgctga tataaccgcc gaggatcggg ccgcaaattg gcgcgacaat
    75001 caccgtcatc gaccacaacg ccagcgcgat cgagcgtttg gctggcgggt agttattcag
    75061 caatagactt tgcgaaagcg ggatcaacgg cccggcgaca atcccctgaa tcacgcggaa
    75121 gaagatcagc atattcaggc tgctggagac accacacgcc cacgacgcaa tagcaaaggc
    75181 gatggtggac caaaggaaca gtttcacttc cccgacgcgc tttgccagcc agccggtaag
    75241 cgggatcgag atggcattcg ccaccccgaa agaagtgatt acccacgttc cctggctgag
    75301 cgatgagccc agattcccgg cgatagtggg gatcgccacg ttagcaatgg tggagtccag
    75361 cacctgcatg aatgtcgcca gtgacagcgc aatcgtcata atgaccagtt gcgcgccttc
    75421 cagcggtttt tgctgttgca tcacacgcac ctctggatta gccagcgtta gcttttacga
    75481 tatcgtcgat cagtttattg acaggtgcca ggctgatttc acgcgcggta cttaccgcga
    75541 ccggtgttga acgtacttta tttgccagta cctgaccgtc acggttagtg gtattgacgc
    75601 tcaccagcgt ggacaaacca atgcgcagcg ggtattgctc cagctgtttc tggtccagtt
    75661 cgatacgcac aggtagacgc tgaacgactt tgatccagtt accggtcgca ttttgcgccg
    75721 gaagcagtga gaacgcgcta cctgtgccca tatccagacc aaccacttta ccggtgtatt
    75781 tcacatcatc gccgtaaata tcggtggtga tggtgaccgg ctgaccgatg cgcatattgg
    75841 caatctgcgt ctctttaaag ttggcatcca cccacatatt ggtggctgga acgaccgcca
    75901 tcagcggcgt cgttgggcta atttgcgcac ccggttgcac ggcacgacgg gaaacataac
    75961 cagtcatcgg actgacaata cgggtacgct ccagcgccag ccaggcgtta cgtacttcgg
    76021 tggcagcctg ttgcacggca ggctgatctt ccagtttagt ccccagaatc atcgcctgat
    76081 tggcattgta ttgttgaata gcgacgtcca gttgcgcctg ggcactggtg acggcgtcgc
    76141 gggcgtgttg cagctcttcg cgaccaatca ggttggcatt gcccagcggc acacggcggt
    76201 tgtagtcgct ttgtgctttc gcgagggcga ttttctgcac ctcaatattc gcctgcaact
    76261 gcttgctgtt aatcatcagc tggtgggttt ggcgaacgct ggaagccagt gcagttttgg
    76321 ctttttcaaa cgcctggcga gcatctgtcg ggtcgagagt gaccagaaca tcgccttctt
    76381 ttacaaaatc ggtgttatcg gcccagactt tcgtcacgct gccagacacc tgagacataa
    76441 tttgaatctg attccctgcc acgtatgcgt catcggtttc ttcgaaatga cgcagtacca
    76501 aaaaccaata aatccctatc gctacggcaa taattataaa gagcaaggtg agaaggagga
    76561 gcagacgctt acgtttgccg ctcttcttta ccggttgctg cggggtttga gtctccgcat
    76621 ttgcgctcat attgttctcc acgatcttct taactcatcg gctgagccga cctgcttgcc
    76681 aaaaaggcca gcatgttgcg atgctggcca gtcatttttt cttttataaa tctggatttt
    76741 tgagcgagat gacgcgttag ctcatcgctt cgagaaccac accgtcttgt tccatctggt
    76801 cgagacggga gagcaatttg cgggtgattt gctcgagctg atctttttct gttgtgctga
    76861 gcgcggacca gagttgatgc aggcagttat gctgcggtgg taaaacctcg cgcaaaaact
    76921 cgtgaccttt ttccgttaat tgcagatgca ggcagcggcg atcgttatcg ctttcacgac
    76981 gttcgatcca accgcgtttt tccagttcat cggcaatacg cgtcgcgttg gtacgggatg
    77041 atccaagagc acaacttaat tcagaaggct gaatactgtg gttttcctga gactccagcg
    77101 taatcaacgc cataaacaac gtctcgttaa tcccctgagc cttcagcatt ttattgcggt
    77161 tctccagcag cttgctttgc atgtgcatgc aaagacgagt cagaaggatc tcctgataag
    77221 gaaaatcttc gtggcggctg gcgcgaaatt ttagcatttg ttcaatgggc gtaaacgaac
    77281 tatccatttg ggtatgacct cattaatttc agccgatata gtaacgacag tgacaaataa
    77341 agtaaatgta ttgttttaga aaaatgattc ttgtggatta tataatcgcc atcactttcc
    77401 agacgagccc gtaggtcagc gcactaagca gtgttgggat gataatgctg cgtgttttat
    77461 agaaactggc acccagtacc gcgaagccga ccagcgtggg cacgaaacgg cgtgtatcgt
    77521 gcatcacttc tggtgcggta gagacaacca gcagagcgca tatcgaggcg atgccaatgg
    77581 tgtcgagcaa aatacctacc gcgccacgtt tggttgggcg ggcattaccc acacgcaggc
    77641 gcagcggcaa atagcggaag caataattcg ccgcgccaac cagtaaccca agcagcagaa
    77701 cctcatagct catcgggcgc tccttgccag aatgcctgga ttaacgcagt gaggcagcca
    77761 cagacaatgc ctgccagaat ggcgacggga atagaaaata gcgttacgcc tgcaagggca
    77821 ccaactaacg ctgcggtaac gcaaagagat tgtttgcgct ggaaagaggc gagcaggaaa
    77881 ctcataaaga gtgccggaag cataaaacct aatgctgctt caacggcggg ataaccttgc
    77941 agcaagccgc tgccggagaa tgcccctatt accgtaccaa atacccacga tgaccatgaa
    78001 ctgaaggcaa tgccgatcat ccagttctcg ctccagcggc gattattgcg taccagtttt
    78061 gcggttgcgg cggcaaaaac ctcatccgtc aggccaaacg cccacagggc ggttttcgat
    78121 ttttgcagac gctgaataat acggctacgc agtgacgggc catacaacac atggcgaaca
    78181 tccattgcca tgacggtcag tgcagcaacc cacaaactac tcccggctgc cagcattgcg
    78241 gtaatgacga actggctcgc gcctgcataa atgatgcagg agaaaaaaac gctttcgaga
    78301 ggagagaatc ccagacgggt cgcattcaga ccgaacgcaa aggccaccgg aatataacta
    78361 ataacaatcg gtaaactgtc tttgcatcct tccatgaagg tcgccgaacc aggagcaggc
    78421 tgtggagtag ggctttccat agagtgtacg cttaacgatt gttaatatta taaatagact
    78481 gaatgaatat cttaacctta tcagactgat gggcttctta acacccttat aagtgtaaag
    78541 ccacgaaaac ggttgctgat tgcttaattt gcggcttcct gacgatgaaa acctcgccac
    78601 cagaccagta agttaaccag ggcaatcgtc gcgccagcca gacaaacgcc agcccaaccg
    78661 ccatgttgcc aggctgaggc tgaaattagc gaaccggcgg caccgccaat aaagtagcta
    78721 gtcatgtaac ctgcggtcag gcgattacgc gcatcaggat gtatccgata aattaccgtc
    78781 tggttagtga tatgcacgcc ctgcacggtg agatccagca ccaggattcc gataatcaac
    78841 gccagtacgg aagtgtgacc aaaccagatc gccagccatg aaagtaatag cagcagcaga
    78901 ccgaaagttg tggtgtggtg cgatttgccc ttatcggcaa aaccgcccgc cggacgagcg
    78961 cccaacgctc cggcagctcc cgcaagtcca aacagaccaa ttacaccatc gctgtagtta
    79021 aaaggtggag cggcaagcaa aaaggccatt gaggtccaga gaatgctgaa attggcaaag
    79081 gtcaggcagc ccagcaacgc gcgggtacgc agaattttat cgctgataaa catactgaaa
    79141 acggaaccca acaactgtgg gtagttgagg tgggtttctg atttcatttg tggcagacca
    79201 cgccataatg ccagcgccat cagtgccatt aacaccgaag caacccaaaa gacggtgcgc
    79261 cagccgccga gattcgccag caatccggca actgtccgtg ccagcaagat ccccaacagc
    79321 agaccgctca taatagtgcc aaccactttg ccgcgtttgt ccggtgaagc cagcgtcgct
    79381 gccagcggaa ccagaatttg tgccacgaca gagaataaac cggttaatgc cgtaccgagg
    79441 atcatcatcg ccagcgactg actgctggca gtgattaaca tgccgcctgc cgccagtaag
    79501 gtcatcgaga caatcaggcg gcggcgttca aacatatcac cgaggggaac aagaaacagc
    79561 agacctgctg catagcccaa ttgcgcggcg gtaacaataa agcctgccga actggcggaa
    79621 agggaaaagt tacgcgcgat ggtgtcgagc aatggctgag cgtaatagtt actggcgacc
    79681 gccagaccgg tggcgataga catcagcacg atcagcgccg ggctaagctc atgattaggt
    79741 ttagtcatta tttttgttca tcaggtgatg attaagctgg aaatcaataa tagcggatgt
    79801 tgatgaatat gggatgaaag atagcttgtt gaaatgtgcg atgtggtttg taggcatgat
    79861 aagacgcgcc agcgtcgcat caggcattgt gcacagaacg gcggatgcgg cgaaaacgcc
    79921 ttatccgcca tacggaatga gtgtattttg taggcatgat aagacgcgtc agcgtcgcat
    79981 caggcattgt gtacagagcg gcggatgagg caaaaacgcc ttatccgcct gaataaaaat
    80041 tacttctgcg ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg
    80101 atccagccat caacgtggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg
    80161 gcgttctggg cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct
    80221 gccgggtttt tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag
    80281 ccataattcg caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag
    80341 aacggcacct gcaaccagac gacatctttg cccggcttca gttcgttact cacccagtac
    80401 ggcgtccagg tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc
    80461 atcatcgccg cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca
    80521 agctggtggt tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct
    80581 tttccgtcgc cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg
    80641 ttggtgattt tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg
    80701 ttaacaaata ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg
    80761 tcatgcagtg gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg
    80821 tagccaacgt tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc
    80881 gcacgactga ccagcagcgt ctggaaggtt tcttcggtga tggtgctttg aactggatta
    80941 acagtaatac ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca
    81001 aacgctgtcg caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata
    81061 atgcccggcg tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca
    81121 acagggccag tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc
    81181 tgcgtcaggc gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca
    81241 agccccatat ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca
    81301 atcatcgagg caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc
    81361 ataatggtcg gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca
    81421 ccgaatgagc gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc
    81481 agacggataa tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg
    81541 ataccaaata gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca
    81601 tcaagcagtg gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccaac
    81661 ggcaaaccga tgacgataca gaacagcagg gcggttaaca ccagcgccag agtaaccatt
    81721 gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc
    81781 attccgaccc cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg
    81841 ggcataccca gcagcaactg ctggaaaccg ttgaggatat aatcaaccgg aacgcgcacg
    81901 ccctggaaga cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg
    81961 tcgagcggga tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc
    82021 gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta
    82081 ccccaggcgt ctgcggattg cgcggcactg tccgccgctg gcgtggtatc ccacggatta
    82141 ttttgatcag ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga
    82201 aatgatgccg acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc
    82261 gacatgagag agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat
    82321 cagcgccgca tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggagac
    82381 tgcgccgaca aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg
    82441 caataatttc agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg
    82501 tgtccggcgg gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa
    82561 ggtacggaca taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac
    82621 cacttcacca ttttgcataa tggcaattcg gtcgccaata cgcatagctt catcgagatc
    82681 gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg
    82741 catctcggtg cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc
    82801 cggattaatc gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc
    82861 atccgggtag ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt
    82921 ttcccggcgt tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag
    82981 cacggtcata tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg
    83041 cacctcacgg agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg
    83101 cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc
    83161 ggataatccc atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac
    83221 gccaagcgat agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata
    83281 tttg
//
LOCUS       DAPHJE010000004        78572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPHJE010000004 DAPHJE010000000
VERSION     DAPHJE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.004"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..610)
                     /locus_tag="RH222_000311"
     CDS             complement(<1..610)
                     /locus_tag="RH222_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV3795379.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNE"
     gene            877..2376
                     /gene="ansP"
                     /locus_tag="RH222_000312"
     CDS             877..2376
                     /gene="ansP"
                     /locus_tag="RH222_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV3795380.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2491..3552)
                     /locus_tag="RH222_000313"
     CDS             complement(2491..3552)
                     /locus_tag="RH222_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV3795381.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3794..5896
                     /locus_tag="RH222_000314"
     CDS             3794..5896
                     /locus_tag="RH222_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV3795382.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5932..6597)
                     /gene="mcbR"
                     /locus_tag="RH222_000315"
     CDS             complement(5932..6597)
                     /gene="mcbR"
                     /locus_tag="RH222_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV3795383.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6795..7832)
                     /gene="curA"
                     /locus_tag="RH222_000316"
     CDS             complement(6795..7832)
                     /gene="curA"
                     /locus_tag="RH222_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV3795384.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPNYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     TRLQGLIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8013..8531
                     /gene="mddA"
                     /locus_tag="RH222_000317"
     CDS             8013..8531
                     /gene="mddA"
                     /locus_tag="RH222_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV3795385.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8528..8977
                     /locus_tag="RH222_000318"
     CDS             8528..8977
                     /locus_tag="RH222_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3795386.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8978..9211)
                     /locus_tag="RH222_000319"
     CDS             complement(8978..9211)
                     /locus_tag="RH222_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV3795387.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9297..9470)
                     /gene="ortT"
                     /locus_tag="RH222_000320"
     CDS             complement(9297..9470)
                     /gene="ortT"
                     /locus_tag="RH222_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV3795388.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9665..9760
                     /gene="yncL"
                     /locus_tag="RH222_000321"
     CDS             9665..9760
                     /gene="yncL"
                     /locus_tag="RH222_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV3795389.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(9857..11281)
                     /gene="patD"
                     /locus_tag="RH222_000322"
     CDS             complement(9857..11281)
                     /gene="patD"
                     /locus_tag="RH222_000322"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV3795390.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGMYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11303..12097)
                     /locus_tag="RH222_000323"
     CDS             complement(11303..12097)
                     /locus_tag="RH222_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3795391.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12087..13028)
                     /locus_tag="RH222_000324"
     CDS             complement(12087..13028)
                     /locus_tag="RH222_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3795392.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPSVLIALYLAFVKRLGAFDAL"
     gene            complement(13029..14042)
                     /locus_tag="RH222_000325"
     CDS             complement(13029..14042)
                     /locus_tag="RH222_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3795393.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGTISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14060..15205)
                     /locus_tag="RH222_000326"
     CDS             complement(14060..15205)
                     /locus_tag="RH222_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3795394.1"
                     /translation="MSKTFARSSLCALTMTIMNAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15450..16856)
                     /locus_tag="RH222_000327"
     CDS             complement(15450..16856)
                     /locus_tag="RH222_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV3795395.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(16935..17351)
                     /gene="hicB"
                     /locus_tag="RH222_000328"
     CDS             complement(16935..17351)
                     /gene="hicB"
                     /locus_tag="RH222_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV3795396.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17397..17551)
                     /locus_tag="RH222_000329"
                     /pseudo
     CDS             complement(17397..17551)
                     /locus_tag="RH222_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            17773..18003
                     /locus_tag="RH222_000330"
     CDS             17773..18003
                     /locus_tag="RH222_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV3795397.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(18095..20056)
                     /gene="rlhA"
                     /locus_tag="RH222_000331"
     CDS             complement(18095..20056)
                     /gene="rlhA"
                     /locus_tag="RH222_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV3795398.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20129..20665)
                     /gene="sutR"
                     /locus_tag="RH222_000332"
     CDS             complement(20129..20665)
                     /gene="sutR"
                     /locus_tag="RH222_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV3795399.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20718..21932
                     /gene="ydcO"
                     /locus_tag="RH222_000333"
     CDS             20718..21932
                     /gene="ydcO"
                     /locus_tag="RH222_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV3795400.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(21972..23180)
                     /locus_tag="RH222_000334"
     CDS             complement(21972..23180)
                     /locus_tag="RH222_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3795401.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            23188..23694
                     /gene="tnpA"
                     /locus_tag="RH222_000335"
                     /pseudo
     CDS             23188..23694
                     /gene="tnpA"
                     /locus_tag="RH222_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(23712..24380)
                     /locus_tag="RH222_000336"
     CDS             complement(23712..24380)
                     /locus_tag="RH222_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV3795402.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(24682..25275)
                     /gene="tehB"
                     /locus_tag="RH222_000337"
     CDS             complement(24682..25275)
                     /gene="tehB"
                     /locus_tag="RH222_000337"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV3795403.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(25272..26264)
                     /gene="tehA"
                     /locus_tag="RH222_000338"
     CDS             complement(25272..26264)
                     /gene="tehA"
                     /locus_tag="RH222_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV3795404.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            26388..27368
                     /locus_tag="RH222_000339"
     CDS             26388..27368
                     /locus_tag="RH222_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV3795405.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(27363..27899)
                     /gene="rimL"
                     /locus_tag="RH222_000340"
     CDS             complement(27363..27899)
                     /gene="rimL"
                     /locus_tag="RH222_000340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV3795406.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(27962..28186)
                     /locus_tag="RH222_000341"
     CDS             complement(27962..28186)
                     /locus_tag="RH222_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV3795407.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(28326..29981)
                     /gene="opgD"
                     /locus_tag="RH222_000342"
                     /gene_synonym="mdoD"
     CDS             complement(28326..29981)
                     /gene="opgD"
                     /locus_tag="RH222_000342"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV3795408.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSTGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(30206..31549)
                     /locus_tag="RH222_000343"
     CDS             complement(30206..31549)
                     /locus_tag="RH222_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3795409.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            31766..32689
                     /locus_tag="RH222_000344"
     CDS             31766..32689
                     /locus_tag="RH222_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3795410.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(32727..34367)
                     /gene="trg"
                     /locus_tag="RH222_000345"
     CDS             complement(32727..34367)
                     /gene="trg"
                     /locus_tag="RH222_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV3795411.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            34766..34915
                     /gene="hokB"
                     /locus_tag="RH222_000346"
     CDS             34766..34915
                     /gene="hokB"
                     /locus_tag="RH222_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV3795412.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(34987..35160)
                     /locus_tag="RH222_000347"
     CDS             complement(34987..35160)
                     /locus_tag="RH222_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795413.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(35405..35935)
                     /gene="cybB"
                     /locus_tag="RH222_000348"
     CDS             complement(35405..35935)
                     /gene="cybB"
                     /locus_tag="RH222_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV3795414.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            36124..37125
                     /gene="gap"
                     /locus_tag="RH222_000349"
     CDS             36124..37125
                     /gene="gap"
                     /locus_tag="RH222_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV3795415.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(37167..38606)
                     /gene="aldA"
                     /locus_tag="RH222_000350"
     CDS             complement(37167..38606)
                     /gene="aldA"
                     /locus_tag="RH222_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV3795416.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(38803..39603)
                     /locus_tag="RH222_000351"
     CDS             complement(38803..39603)
                     /locus_tag="RH222_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV3795417.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(39875..43777)
                     /gene="hrpA"
                     /locus_tag="RH222_000352"
     CDS             complement(39875..43777)
                     /gene="hrpA"
                     /locus_tag="RH222_000352"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV3795418.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEAVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            43978..44583
                     /gene="azoR"
                     /locus_tag="RH222_000353"
     CDS             43978..44583
                     /gene="azoR"
                     /locus_tag="RH222_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV3795419.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(44634..45950)
                     /locus_tag="RH222_000354"
     CDS             complement(44634..45950)
                     /locus_tag="RH222_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV3795420.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(45940..47697)
                     /locus_tag="RH222_000355"
     CDS             complement(45940..47697)
                     /locus_tag="RH222_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV3795421.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISHLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRMAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALENEPAVCDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(47713..48609)
                     /locus_tag="RH222_000356"
     CDS             complement(47713..48609)
                     /locus_tag="RH222_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV3795422.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            complement(48609..49214)
                     /locus_tag="RH222_000357"
     CDS             complement(48609..49214)
                     /locus_tag="RH222_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV3795423.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(49513..50373)
                     /gene="pdxI"
                     /locus_tag="RH222_000358"
     CDS             complement(49513..50373)
                     /gene="pdxI"
                     /locus_tag="RH222_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV3795424.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(50604..51194)
                     /gene="paaY"
                     /locus_tag="RH222_000359"
     CDS             complement(50604..51194)
                     /gene="paaY"
                     /locus_tag="RH222_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV3795425.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLATRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(51176..52126)
                     /gene="paaX"
                     /locus_tag="RH222_000360"
     CDS             complement(51176..52126)
                     /gene="paaX"
                     /locus_tag="RH222_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDV3795426.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVVDNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(52227..53540)
                     /gene="paaF"
                     /locus_tag="RH222_000361"
     CDS             complement(52227..53540)
                     /gene="paaF"
                     /locus_tag="RH222_000361"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDV3795427.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(53567..54772)
                     /gene="paaJ"
                     /locus_tag="RH222_000362"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(53567..54772)
                     /gene="paaJ"
                     /locus_tag="RH222_000362"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDV3795428.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(54772..55194)
                     /gene="paaI"
                     /locus_tag="RH222_000363"
     CDS             complement(54772..55194)
                     /gene="paaI"
                     /locus_tag="RH222_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDV3795429.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(55184..56611)
                     /gene="paaC"
                     /locus_tag="RH222_000364"
     CDS             complement(55184..56611)
                     /gene="paaC"
                     /locus_tag="RH222_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDV3795430.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(56613..57401)
                     /gene="paaB"
                     /locus_tag="RH222_000365"
     CDS             complement(56613..57401)
                     /gene="paaB"
                     /locus_tag="RH222_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDV3795431.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(57401..58168)
                     /locus_tag="RH222_000366"
     CDS             complement(57401..58168)
                     /locus_tag="RH222_000366"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDV3795432.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(58165..59235)
                     /gene="paaK"
                     /locus_tag="RH222_000367"
     CDS             complement(58165..59235)
                     /gene="paaK"
                     /locus_tag="RH222_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDV3795433.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIAATLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(59243..59740)
                     /gene="paaD"
                     /locus_tag="RH222_000368"
     CDS             complement(59243..59740)
                     /gene="paaD"
                     /locus_tag="RH222_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDV3795434.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(59755..60501)
                     /gene="paaC"
                     /locus_tag="RH222_000369"
     CDS             complement(59755..60501)
                     /gene="paaC"
                     /locus_tag="RH222_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDV3795435.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(60510..60797)
                     /gene="paaB"
                     /locus_tag="RH222_000370"
     CDS             complement(60510..60797)
                     /gene="paaB"
                     /locus_tag="RH222_000370"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDV3795436.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(60809..61738)
                     /gene="paaA"
                     /locus_tag="RH222_000371"
     CDS             complement(60809..61738)
                     /gene="paaA"
                     /locus_tag="RH222_000371"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDV3795437.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            62023..64068
                     /gene="paaZ"
                     /locus_tag="RH222_000372"
     CDS             62023..64068
                     /gene="paaZ"
                     /locus_tag="RH222_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDV3795438.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            64316..66589
                     /gene="tynA"
                     /locus_tag="RH222_000373"
     CDS             64316..66589
                     /gene="tynA"
                     /locus_tag="RH222_000373"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDV3795439.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEAEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNTVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(66647..68146)
                     /gene="feaB"
                     /locus_tag="RH222_000374"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(66647..68146)
                     /gene="feaB"
                     /locus_tag="RH222_000374"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDV3795440.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            68382..69287
                     /gene="feaR"
                     /locus_tag="RH222_000375"
     CDS             68382..69287
                     /gene="feaR"
                     /locus_tag="RH222_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDV3795441.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(69459..69788)
                     /locus_tag="RH222_000376"
     CDS             complement(69459..69788)
                     /locus_tag="RH222_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV3795442.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(69793..69978)
                     /locus_tag="RH222_000377"
     CDS             complement(69793..69978)
                     /locus_tag="RH222_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV3795443.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(69975..72614)
                     /locus_tag="RH222_000378"
     CDS             complement(69975..72614)
                     /locus_tag="RH222_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV3795444.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQSPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            72822..73811
                     /gene="ldhA"
                     /locus_tag="RH222_000379"
     CDS             72822..73811
                     /gene="ldhA"
                     /locus_tag="RH222_000379"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV3795445.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            73922..74344
                     /gene="hslJ"
                     /locus_tag="RH222_000380"
     CDS             73922..74344
                     /gene="hslJ"
                     /locus_tag="RH222_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV3795446.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(74341..74607)
                     /locus_tag="RH222_000381"
     CDS             complement(74341..74607)
                     /locus_tag="RH222_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV3795447.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            74881..78405
                     /gene="nifJ"
                     /locus_tag="RH222_000382"
     CDS             74881..78405
                     /gene="nifJ"
                     /locus_tag="RH222_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV3795448.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRIRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        19494 a        20607 c        19023 g        19448 t
ORIGIN      
        1 tttcattcct ttttaatact tcttgtaatt tcggtaactg acatacagca tcagcaatcg
       61 cactgatatt cgtggcatta tcctgaaacc attcatgccc aggcccccat gtgcgcatag
      121 tgcaaagata acagtccacc agcgttagtt gctcaccaaa cgcataaggt tcagcggtca
      181 attgtgaatt cagccagata taaagcgatt tacgatattc aataacattc ttctttaact
      241 gttcaggtgc gtcaggagcc caacgctcag ggtaatcggc gaaagtgaat gttggataga
      301 cattagcaac cagccagacc aatagccgtt gaaactgctg acgttcggcg cgcccaaccg
      361 gtggcgcaag gtccgggcga cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca
      421 taatctcgtc attttccagc gccagtgtcg gaatctgaca caacgggttc agggttttca
      481 ataactcgcg tgaagttccc tcgtggtcaa aaccactgac atcaacgaac tgataaggaa
      541 tatcagccag tgttagcatc agctcactga ttgtagagcc ccatccgggt acgccataca
      601 ccttaatcat atgcccctca caatctttta aagattaagt gtaggacagt acacaggtta
      661 accagcaaaa ggatttgtca ttttttcgtc agttgtatta atccccttca ggattgtaag
      721 gggatgtttt gcatcgttta tcgataacgg gtaggatgct gcgccgatag ttttataccc
      781 ataaagaata atggtgataa ctatcatcgc caggatgaat aaacattgtt catggcaact
      841 tatatgactt tttcattaaa gcaatcaggg agagcaatga gtaaacacga caccgacact
      901 tcagatcaac acgccgcgaa acgccgctgg cttaatgccc acgaagaggg gtatcacaaa
      961 gcgatgggca atcgccaggt acagatgatc gccattggcg gcgcgattgg caccggcttg
     1021 tttttaggtg caggagcccg actgcaaatg gcgggtccag cactggcact ggtttattta
     1081 atttgtggct tgttttcgtt ttttattctg cgtgcattgg gtgagctggt gctacaccgc
     1141 ccttccagtg gcagttttgt ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat
     1201 gttgctggct ggatgtactt catcaactgg gcgatgacgg ggattgttga tattaccgcc
     1261 gtcgctctgt atatgcatta ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg
     1321 ctcgctgcac ttaccatcgt tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag
     1381 atggagttct ggtttgcgct tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt
     1441 acagtgttcc tcggtagtgg tcagccgctg gatggcaaca ccactggctt tcatttaatc
     1501 accgataatg gcggcttctt cccccacggt ttgctgcctg cgctggtgtt gattcagggc
     1561 gtagtgtttg cttttgcctc cattgaaatg gtgggtacag ctgccggaga atgtaaagat
     1621 ccgcagacca tggtgcctaa agcgattaac agcgtgattt ggcgtattgg cctgttttac
     1681 gtcggctccg tggtgttgct ggttatgcta ttgccgtgga gcgcgtatca ggcggggcaa
     1741 agtccgttcg tgacgttttt ctctaaactg ggtgtgccat atatcggcag cattatgaac
     1801 attgtggtgc tgaccgctgc cctctccagc ctgaactcag gtctgtactg caccggacgt
     1861 attctgcgct caatggcgat gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt
     1921 cagcatgtgc cgtatgccgg gattctagca acactagttg tgtatgtcgt cggcgtattc
     1981 ctcaactatc tggtgccgtc gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga
     2041 atcatcgctt catgggcgtt tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa
     2101 gaaggcaaag cagcggatgt cagttttaaa ctgcctggcg cgcccttcac ttcctggctg
     2161 acattactgt ttttactgag tgtccttgtg ctgatggcgt tcgattaccc gaacgggact
     2221 tatactatcg cggcgctgcc gattatcggt attctgctgg ttataggctg gtttggtgtg
     2281 cgcaaacgcg ttgctgaaat tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa
     2341 caggaaatcg tgtttaagcc tgaaacggcg agctaattgc aaaattgccc ggctacgtgc
     2401 cgggcaattt caaattactg acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa
     2461 gtctctccgt tttcaccctc gccatatcta ttacagcgca atacgaatca catcgtccgg
     2521 ctgggtagct tcctgctgtt tagtggattt ttgttttaca ctgacataca gcgttttgcc
     2581 atcggcagac agcgccaggc tgttcggatg agtcggcgta tcgaacgttt tcaccacttt
     2641 atagcttttc gcgtcaatca cactgacttt acctgcctga cgatgcgtta cgtaggcttc
     2701 attacgcgct gggttaaaca gcacagccag tgattccggt gccgcaacct tcgccagaat
     2761 attgccatta cgggtatcga ccactaacac ttctgccgct ttagaatcgg tgataaatgc
     2821 acgctgcctg gcggtatcaa ggctaatgtt gataaagaag tgctctttgc cgtcatccag
     2881 cagcttttta cggctgagga ttttattgtc ggcggtgtcg atggtaatca attcgccgtc
     2941 agcgttagtg gtgtaaagac gtttgccttt gctatccagc gccagaccgg tactcatttt
     3001 accggtgttc tggatggcgg ttttcagttt gatattctcg ccatcaacga cccaaatcac
     3061 gctctcttta ccaataccac tgatgtaaac ggtgttcgtg gcatcatcag ctaccagctc
     3121 acgcggttgc agcgggcgca cctcttccgt gcgcttacga tcatccagca ccagacggcc
     3181 tttcacttcg cccgttttgg catctatcgc cgtgaccgcg ctgtttacgg tgttaccaaa
     3241 ccacaacgtc tgagtcgtgt tattgatggt ggcaccaaac ggcttgagat cgttatggat
     3301 cgcctgcgtc acttccagag tgaccggatc aagacgataa accacgccac ctttatccag
     3361 tttgcggctt tgcgaagtgg cgagccacag cgcgttttct tgctggctat aagccatttc
     3421 gtaggcacct ttacctaccg ctttacgcag catttcttct gcggcctgcg tactgaatga
     3481 tgaagcaaca agcaatgaac ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg
     3541 acgtaaatgc atgacgactc cctttgatga accaatattt ttgctgcttc acaatgcttc
     3601 gctaagcgta atcatcgtta cgctcttgtt ataaatggga atggtaatca ttattttctc
     3661 aaatgtggag catttcttgc cgttacgact acaattttct caattaatta acgtatcgtg
     3721 ccgtttcgtt ttccataaat ttacaatttt attaccatga cagcgttttc ccattcttaa
     3781 cctggctttt ttcatgaaga ttttttccgt ccgacagacc gttttgcccg cactgcttgt
     3841 cctttccccc gttgtttttg ccgctgatga acagactatg attgtcagtg ccgcaccgca
     3901 ggtggtttcc gagctggata cgccagcagc agtaagcgtg gtggatggcg aggagatgcg
     3961 cctggcaaca ccgcgcatta acttgtccga atcactgacc agcgtgcctg gtttgcaggt
     4021 acaaaaccgg cagaactatg cgcaagattt acagctgtcg attcgcggat ttggctcccg
     4081 ttccacttac ggaattcgcg gtattcgcct gtatgtggac ggtattcccg ccaccatgcc
     4141 cgacgggcaa gggcaaacat ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct
     4201 gcgtggcccc ttctctgccc tgtatggcaa cgcgtctggc ggtgtaatga atgtcaccac
     4261 ccagaccgga caacagccac caaccattga agccagtagt tactacggca gttttggcag
     4321 ctggcgctat gggctgaaag caacgggcgc aacgggagac ggtacacagc ccggcgatgt
     4381 cgattacacc gtctcaacca cgcgttttac gacccacggc tatcgtgacc atagtggcgc
     4441 acagaaaaat ttagccaatg ccaaactggg cgtacgcatt gatgaagcca gcaaattaag
     4501 cctgattttc aatagtgtgg atatcaaagc agatgaccca ggtgggctaa ccaaagcaga
     4561 atggaaggcg aatccacaac aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat
     4621 caagcaaact caggctgggt tgcgctatga gcgtagcctg agcgcgcaag atgatatgag
     4681 tgtgatgatg tatgccggag agcgagaaac gacccagtac cagtcaatcc cgatggcccc
     4741 ccaacttaac ccgtcacatg cgggcggcgt gattaccctg caacgccatt accaggggat
     4801 agacagccgc tggacacacc gtggtgaact gggcgttccg gtcacgttca ctaccggcct
     4861 gaactacgaa aacatgagtg aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg
     4921 catgccggag tacgggcaaa aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa
     4981 catcgatccc tatttacaga cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg
     5041 cgtgcgctac agctccgtgt ggtttgattc caacgaccat tacgttactc cgggtaacgg
     5101 cgatgacagc ggtgatgcca gttatcataa atggctacct gccggttcgt taaaatatgc
     5161 aatgaccgat gcctggaata tctatctggc agccgggcga ggttttgaaa cgccgacgat
     5221 taatgagctg tcttatcgtg ctgatgggca aagcggtatg aacttaggtt taaaaccatc
     5281 caccaacgat acaattgaga tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct
     5341 cgcattgttt cagaccgaca ctgatgatga aattgttgtc gatagcagta gcggtgggcg
     5401 tacgacttac aaaaatgccg gaaagacccg tcgtcaaggc gctgaactgg catgggatca
     5461 acgtttcgca ggagattttc gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg
     5521 cagcaatgtt tgcaatgaac aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa
     5581 tatgggcttt gcgtcgatag gttatgtacc ggaagatggt tggtatgcag gcacggaagc
     5641 gcgttatatg ggcgatatta tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac
     5701 tctcgtcggc ttattcaccg ggtataaata caattaccac aatttaactg tggatttatt
     5761 tggtcgtgtc gataatttat tcgataaaga atacgttggt tctgtcattg tcaatgagtc
     5821 aaacgggcga tattacgaac cttcgcccgg acgaaattat ggtgtcggca tgaatattgc
     5881 gtggagattt gagtaattac cttgatgccc ggtatttgcc gggcatttac tttaacgatt
     5941 gtattgctgg tataaaatag caatattttg ttgcataatt tcagcaagac aatgtctgca
     6001 tccctctttg tcttttgctt ttaacgcagc gagtaacaga tgatagtttt ctatatgctc
     6061 ccgtaattcc gctggattaa tcgcttcata gagataatgt aaaccaggcc ccatcctgac
     6121 ccacagttgc tcaatcatct cacacaggat gggcatattt gagcgatgat aaatcgctaa
     6181 gcgaaatagc ctgtttacat taatgatttg ttccatgtcg cccttttctt gcgcctgctg
     6241 taatttctcg agcaattcct ggagttccgc aaggtcttgc ggggtgaggt tttcaacagc
     6301 cagagcaact gccattaatt ccagttcgta gcggatccga ttgatttcgt ctaattgacg
     6361 tttccccact tccggaactg taaatgcttg tgcaggtgcg acagaaagcg cattcaccga
     6421 aaccagacgt aataatgctt cacgcacagg tgtaatactc atacctaatt gctccgccag
     6481 atttttagta atcaggcgtg cgccaggttt taatgcgcca atactaagct gatgtttcag
     6541 gtcgttctca acctgcagag tcaaactgac atgtttcatt tttcccgttc caggcatttt
     6601 aattccttcg cgtgttttct gtccaaccct gcctgcatgg cagaaggagt tcacctggtg
     6661 tattaaagtg aataatcata aaccacccac ctttgttgtt attagcagca gaacaatgac
     6721 gtggttagtt aattaaagct gaaaataagc tttttgcctg gatttttatg gcggcttacg
     6781 ccgccgttga aactttaatc atcacccgcg acgcggatca ccactttgcc gaagttttta
     6841 cccttcagca agccgataaa cgtctgtggc gcattctcta aaccatcagt aatatcttcg
     6901 tggtagtgga ttttatcctc tttcacccat tgccccatct ccttctgaaa ctcatggatg
     6961 cggtgaccat aatcctgagc gataataagc ccttgcaagc gagtacgttt tttcagcacc
     7021 gttgccatca acaggggtaa acgatccgga ccgggtggta gctctgtagc gttatagctg
     7081 ctcactaagc cacaaacggg aatgcgcgca gatgtattaa gtaatggtag cactgcatca
     7141 aataccttac cgcccacgtt ttcataatag atatcaatac cttttgggca cgctttcacc
     7201 agttgttcgg caaaatcatc cgcgtggtga tcaagacaaa catcgaagcc cagcacctcg
     7261 atagcatggc ggcatttttc cgcgccaccg gcaaccccca ccaccctgca acctttaagt
     7321 ttgccgattt gccccaccgt tgctcccaca ggacctgtcg ccgcagctac caccaacgtt
     7381 tcgccctctt taggctgacc gatatccagc agccccatat aagcggtaaa gcctggcatc
     7441 cccagcacac ccagcgacca cgatggattt tgcggatgat cgccaagttt caccagatca
     7501 tcaccactgg atatgtcata gtcctgccat ccactgtagc ccagcaccca gtcgccaggc
     7561 tgataattag gatgattcga ctccacaaca cggctcaccg tgccgccgac catcacgccg
     7621 ccaatatcaa caggcggtga ataagatggc tcatcgctca tacgtccacg catatacggg
     7681 tccagggaca aataaactgt gcgcagtaac acctgtcctt cacccggtgt ggcgacatca
     7741 tcttcttcaa gacggaaatt ctccggaacc ggtgcgccat gtggacgcga ggccagaacc
     7801 caacgtcgat tacgctgctt ttgttgcccc ataatgctct cctgtgcttt tggcatgaat
     7861 ccatcaatag tagcaggctc acgggattgg ttttgtttgc agccgtcaca tcccctctgc
     7921 aacacgcaat aatttcatcg aggaatccgt tgcttcgggc tgttttcgat agtattgata
     7981 acatcatcat cattatttta tcgaggccag acatgtccat ccgttttgcc cgcaaagccg
     8041 attgtgctgc cattgcggaa atttataacc acgccgtgtt gtatacggcg gctatctgga
     8101 atgaccaaac ggttgatgca gataaccgca ttgcctggtt tgaagcgcgg accatagcag
     8161 gttatccggt gctggtaagt gaagaagatg gcgtagtgac gggatatgcc tcgtttggcg
     8221 actggcgtag tttcgatggt tttcgccata ccgtggaaca ttcggtttat gtccatcccg
     8281 atcatcaggg caaaggtctg gggcgtaaat tgttaagccg attgattgat gaagcgcggg
     8341 attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca aaatcaggcc tcgctgcatc
     8401 tgcaccattc gctgggattt gtcgtcaccg cacaaatgcc gcaggtgggc actaaatttg
     8461 ggcgctggct ggatctgaca tttatgcagt tgcaactcga cgaacgcact gaaccggacg
     8521 cgatcggatg aatcagtcgc tcacccttgc ttttttgatt gccgccggaa ttggtctggt
     8581 agtccagaac acgctgatgg tgcgcatcac ccagacctcc tctaccattc tcatcgccat
     8641 gttgctgaac tcactggtgg ggattgtgct ctttgtttct attttatggt ttaaacaagg
     8701 catggcgggg tttggcgaac tggtgtccag cgtgcgctgg tggacactta tacccggctt
     8761 gctgggatcg ttttttgtct tcgccagtat cagcggatat cagaatgtgg gagcggcgac
     8821 aactattgca gtactggtcg ccagccagct aattggcggt ctgatgctgg atatcttccg
     8881 cagtcatggc gtaccactac gggcgttgtt cggcccaatt tgtggcgcaa ttttacttgt
     8941 ggttggcgcc tggttggtag ccagacgctc attttaatca gaggatggtg ccgcctttgg
     9001 ttaactgttc gtggcgcgct tccatatctt ctttatgctt gcgaccgtga tgagcaatag
     9061 cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg acttaactcc gcgtcatcgc
     9121 tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc gcggcatcca
     9181 cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttagccactt gtgaaattta
     9241 tcgattcatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag taagatttaa
     9301 aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgttgcccc caccagaaag
     9361 gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat aagaaatgcg
     9421 attgctgcca taaccgcata aaaaacaagc atgtgttgat agagagacat gtacgggcgt
     9481 ccagccagat gaataaaacg cgcgcaaata tgcgtcattt tttgacgtgc atcaagtttt
     9541 ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc cttattcatc ttcagaatga
     9601 gtattattgc ggattttctt aacccgtact atacacatag gggcaatgat aaaaggtggc
     9661 aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg ctgttggttt
     9721 gtttactctt atctctatga gatttggctg gtttatttga tgtagtgatt ttgcacgcag
     9781 cacgtactgt cagagaaatc agtgctctga cagtacgcaa gatcatctca tactcttacg
     9841 atctgcaata ttatccttaa tgtttaacca tgacgtggcg gacgacggtg taatcctcca
     9901 gcccataaag tgacatatcc ttgccgtaac cagaaagttt ctgtccaccg tgcggcattt
     9961 cacttaccag catgaaatgg gtattgaccc aggtacaacc atattgcagc cgtgcgctga
    10021 cgcgatgcgc cctgcccaca tctttcgtcc ataccgaaga tgcaagtccg tactggctgt
    10081 cattcgccca gttcaccacc tgttcttcgt tgtcgaagag cgtaacactc actactggac
    10141 caaatacctc tttttgcacg atggcatcgt cctgtaatgc gccagccagc agcgtcggcg
    10201 catagtaata gccattaccc ttgcgctttt caccgccagt gatcactttg atgtgccctg
    10261 tcgctttcgc ctcttctact gccttgctga cgcgttcgag atgcgccagc gagcttaaag
    10321 gtccaagctc cgtagactcg tcatccggcg caccagattt taacgttgcc accgcagcac
    10381 ccagtttttc caccagcgta tcgtacatgc ctttttgcgc gtagatccga caagccgcag
    10441 tacaatcctg tccagcattg taatagccaa atgtacgtac accttcgacc actgcttcaa
    10501 tatccgcatc atcaaaaaca atcactggcg ctttgccacc aagttccata tgagtacgct
    10561 taatggacga cgcggtatgg ctgatgatgt gctcgccggt ggcgatagag cccgtcagcg
    10621 acaccatccg cactttggga tgaccggtca gcggatcacc caccgttttg cctctgccaa
    10681 acagtacgtt aatcacgcct gccgggaaga tatctttcgc cagctctgcc aacttcaacg
    10741 cggtcagcgg ggtaatttct gatggtttaa gcactacgca gttccctgcc gccagcgccg
    10801 gagccagttt ccacgctgcc atcatcagcg gataattcca cggtgcgata gaagccacga
    10861 cccccaacgg atcgcgacgg atcatcgaag tatgaccttc aagatattca cctgccgcca
    10921 gaccattcag acagcgcgcc gcacccgcga aaaagcgaaa aacatcgaca atcgccggga
    10981 tttcatcatt gaacgcacta tgcagcggtt tgccacaatt acgggactcc agttcggcaa
    11041 aaacctgacc attttcttcg ataacatcag ccagtttcag cagacattcc gcacgcactt
    11101 tcggcgtggt ttgcccccat tcggcaaatg ctgcatctgc cgcgcgcaca gcagcatcga
    11161 cctgctctgc ggatgcctcg gcaatctcca gtaaaacgtc ccccgttgcc ggattataga
    11221 caggctgttt ttccccttcg ccgctaacca gttctccgtt aatcagtaac ttatgttgca
    11281 tagcatattc ctgtatcagt ggttattgac cattgtcgcc ttcgcgggtt agccaccagg
    11341 cccccaggat cggcaaggtt gttaccaaca taaccagcag tgccaccacg ttagttaccg
    11401 gtacatcacg cggtcgccca agctgattga gcaaccacaa cggtaacgtt cgttcatgac
    11461 ctgccgtaaa agtcgtaacg atgatttcat cgaacgacaa ggcaaacgcc agcattcctc
    11521 ctgccagtag cgccgaactg agattcggca acactacgta gcggaaggtt tgccagccat
    11581 tggccccaag atccattgac gcctcaacca gactccacga ggtgcggcga aaacgggcga
    11641 tgacattgtt aaacaccacc actacgcaaa aagtcgcatg accgaccacg atggtgaaaa
    11701 atcccggctc cagattgatg gttttaaaag cggttaatag cgccagacca gtgacaatgc
    11761 ccggcagcgc aatgggcagc agcagtaaca gcgaaatggc gtttttgccg aaaaagtctc
    11821 gtcgccacag cgcggcagct gccagcgtcc ctaacaccag cgccattaat gtcgccagcg
    11881 ccgccacttt aagtgacagt gtcacggcat caagaatatc actacgctgt gctgccacgc
    11941 taaaccagcg cagcgtcagg ccctgcggtg gaaaactaaa cgccgcatct tcagtgttaa
    12001 acgcataagc ggcgataatc aggatgggaa aatgtaggaa aaccacgccg ccccaggccg
    12061 ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga acgctcccag acgtttcacg
    12121 aacgccaggt acagtgcgat gagaacactc ggcaccaggg tgaatgccgc cgccatcggc
    12181 atattgccaa tcgccccctg ctgggaataa accatattgc cgataaaata tcctggagga
    12241 ccaaccagct gcgggacgat aaaatcgccc agtgtgagtg agaaggtaaa gatagagcca
    12301 gcggcaatac ccgggattgc cagcggcagc accacatagc gaaaggtttg tcgtggacgt
    12361 gcgccgagat cagccgacgc ctgcaacaat gacggcggca aacgctcaag cgccgcctga
    12421 acgggcagga tcatgaacgg caaccagata tagagaaaca ccagaaagcg ccccagcccg
    12481 gaagttgaca gcgtatttcc gcccaccgca ggtaatgtaa ggaacgcagt cagcagtggt
    12541 tccagcccaa gatgttgtaa aaaccactga gccacgccat cttttgccag caataacgtc
    12601 caggcatagg ctttaacaat gtagctcgcc cacatcggca acattaccgc aatataaaaa
    12661 aaagctttca ttttcccgct ggtatagcgc gccatatacc acgccattgg aaaagccaga
    12721 atggcgctgg cgatagtgac tgccacagcc atagtcagcg tgcggagaat gatgtcgtaa
    12781 ttcgccggat taaacagcgc acggatattc gccagcgtca gttccggcgt taccgacatg
    12841 gtgaaatcgt caaaagtata aaatccctgc cataacagtg tcagcagcga gccgaaatag
    12901 acaatgccaa accacattag cgggccaagc agcagtaaaa acagccccag ccccggatta
    12961 cgccagaaga aaccggacac cttacccaga cctggacgtg aaggtgattg caatacattc
    13021 atcgccattc acctctcctc aaccagcggc accatcacat cacgcgacca ggaaaccatc
    13081 acctgttgtc cgggcgtgag cgtggcaggc agttcttcgc ctgtcatatt ggcctgactc
    13141 acaagcagtt tttcaccgcc gttcaatttc agttcaaaac gagttgccgc gccctgatat
    13201 tgcaccgcct ggatcgtgcc attggcctgc agttcaccag gggtgttgag gcggatatgt
    13261 tccggtcgca gggcgaagct tcccgtcatg ccacaaagtt tctctgccat cagtccatca
    13321 aaaacgttgg atgtcccgac gaatccggca acaaacggcg tgcgcgggcg catatagaga
    13381 tcgcgcgggg aatcgacctg ctcaatgcgt ccattattga aaaccgccac acgatcggac
    13441 atcgataacg cttcgccctg atcgtgggta acgaagataa aagtgatacc gagagactgt
    13501 tgcagttttt tcagttccag ctgcatctgc tcacgcaatt tgagatccag tgcgccgagc
    13561 ggttcatcca acagcaatac gcgcggttca ttcaccaatg ctctggcgat agcaacccgc
    13621 tggcgctgac caccagaaag ttgtgacggt ttacgttgat gtacaaaccc caacgccact
    13681 ttctccagcg cctcttgcgc cattgcgtgc cgctgctttt tattcacgcc tttgaccatc
    13741 agcccatagg cgacattgtc aagaatcgac atatgcggaa atagcgcgta gtcctgaaag
    13801 acagtattca cgtcccgctc ccacggtggc agattgctgg cgggtttacc aaagatagag
    13861 atagtcccgc cggaaagctg ttcgaagcca gcaatcaggc gcaggcaggt ggttttgccg
    13921 gagccggacg gccccagcat agagaagaac tcaccatctt ttatcgcaat actgacgcca
    13981 tctactgcgc gcacgtcacc gtacaaccgc gagacgttgt caaactccac tgcgtacgtc
    14041 ataaagcacc ccagcgaagt tagcgaccgc ccataatggc aatgtaatcc tgcgtccagc
    14101 gactgtaggg aacaaacttg cccccttctg ctataggcgt tttccagaag gcgattttgt
    14161 cgaaatagtt aaaaccgttg gtttcacaac ctttttcgcc taataacgga ctggctttac
    14221 acccttccgg cactaccggt aacgagccaa accaggccgc cacatcgccc tgcacttttg
    14281 gcgttaatga ccagttcatc catttgtagg cgcaaaccgg atgtttcgct tcgctatgca
    14341 gcatggtggt atcagcccaa ccggtaacac cctccttcgg gaaaacggta gcaacaggct
    14401 ggccttcggc tttcagggcg ttggcctgat agggccaggc actggaagca accacaccct
    14461 cgtttttgaa atcgctcatt tgcacggtag tgtcatgcca gtagcgatgg atcaaactgt
    14521 gttgagcgcg cagcactttc agcaccgcct ggtactgttc ttcggttagt tgatacggat
    14581 cgctgatgcc caactgcggc tgagtggctt taacgaacaa cgcagcgtcc gcaatgtaga
    14641 tagggccatc ataagcctga acgcggcctt tattgctctt gccgtccggc agattttgct
    14701 caacaaaaac cacttgccag ctatccggcg gcgtcgggaa ggttttagtg ttgtacatca
    14761 gcaggttcgg cccccattgg taaggtgtgc cgtaaacttt gccgccaaca ttaaaccagt
    14821 cgcctttaac cacgcgcgga tcgagcgttt tccagttggg aatcaaggcg gtattaatcg
    14881 gctgcacgcg tttgcccata atcaaacgca gcgaggcatc gccggatgcc gtaaccagat
    14941 cgtaaccccc tttggtcatc agactgacca tttcatcgga agtcgcggcg gttttcacat
    15001 tcaccgcgca gcctgtctct ttttcaaatt gcgttaccca gtcgtattgt ttatcagttt
    15061 gtccgcgttc gatgtatccc ggccaggcga taatatccag tcgcccttcc ggtttatcta
    15121 aattggtagg tggttcggcg gcgtgagcgt tcattattgt catggtgagc gcacacaggc
    15181 tgctgcgggc aaatgtcttg ctcataaggt cttactcctg tcgtaattaa ttgttcagcc
    15241 gccgtgccgt aaaaatatct ccttgtttaa ccatagacgg cggcttgcag gcacaccttc
    15301 gcccctgaga aaatttaatc aggttgtgag ctaccgcgca ttatgccgtc tgtaaggtta
    15361 tcttctggag tatagacaag gggttaggaa atatgactgc tattcgaaat tcatattacc
    15421 caatctaaga ggaattaaat taaacgctat tacagccgtt cttgaataag tttgcctaat
    15481 tgttttaccg cctgttcttc acgttctccc cactgccacg cggtattaaa acgaaaaaaa
    15541 cgtgaccagt tttcaccggt agaaaacatt ttacccggcg caatactgat atgatgcgtc
    15601 agtgccgcca ggcttaattc gccggcatct aacggctcgg ggagctccag ccagagaaaa
    15661 taaccgctgt cactatgatg aattttaact tccgcaggca gataacgcag cagtgcctgc
    15721 caggcacgtt gtttacgttc cgcaagctgg cgacgcaggc gacgaagatg ggcgtcgtat
    15781 cggcgcgtgg aaaggtaatc caccagcgca agttgcatcg gtgagctggt ggaaagcgta
    15841 ctcatcaact gcaagcgttg aattttacgt gcatgttttc cggcggcgac ccaaccaata
    15901 cgaaaaccag gcaccagaca tttcgaaaac gaagagcaat gcaaaacgcc atcgtggcga
    15961 tcccacgctt tcgcaggcag cggtttttcc cgtccaaaat aaagttcgct gtaaacgtca
    16021 tcttcaatca gcgttacgtt gtactgattg agtaacgcta ccagttgtgc ttttttttgc
    16081 ggcgttaagg taaatccgag tgggttctgg ctattagtca tcagccagca cgctttcacc
    16141 ggataatcct gcaacgccag ttccagcgcc tgaagatcga tcccttcttt aacatccgtc
    16201 gccaccgata acgccttcag ccgtagccgc tccagcgcct gcaacgcacc gtagaaacaa
    16261 ggattctcta ctatcaccca atcgcccggt tcagttaccg cttgcaaact gaggtttaat
    16321 gcctctaacg ccccggcagt aatgacaatt tcatcaggag aaatggtgat gccctgtaag
    16381 gcatagcgac gagcaatagc ctgacgcagt tctgcgtttc ctggcggtaa gttttcaatc
    16441 acgctcatcg ctgtggcggt tttgcttacc tgcgccagcg agcggtttag ttgttggagg
    16501 gggaaaagtc gcgggtcggg aaaggccgag gcaaacggaa cgaccgacgg atcgcgactg
    16561 gcctgcaaca tatcaaaaat ataagtgttg atatcgactg cttcatctcg agtgactgga
    16621 atgactggcg ctttcggcat ttttattgcc tgtggcgcaa cgtaataacc cgactgcggt
    16681 cgtgcgataa tatatccctg actttcgagc aactgatagg catggctgac agtcataaag
    16741 ctcatgcctg aaagcgccac ctggtcacgc aacgaaggca aacgatcgcc gggttgccag
    16801 atacccgacg caatctgctc gcgtaattgt tctgcaagct gctggtattt tttcattatc
    16861 gaatcgtaat tatgtgcaga tgattcggca gtctatatca gttgttaaaa atgacaactt
    16921 ttcgttaact gtaattaaac catcaccagc gataactctt tgccaagcgc ctttgccgca
    16981 agctggacgg cgtcgatttt tgtcgcatgg tgcaagttaa ataggcgagt aatctcctgt
    17041 ttaggtttgc caattcgcct ggctaactct tgctgagtga tttctgactg taaaaaagca
    17101 tttaacagca atactttaga ggcgacgctc aaaggtactt caataaagtg atcatgacta
    17161 tttaatggcg aaggtaacgg gataagctcg ttatcttcaa aataaaaatc aaatgcggtc
    17221 agtaaagcat cttttgccgc ttccatcgct tcagcgacag tttcgccctg cgtcaacgct
    17281 tcagggatat ccacaaaaga aaccatataa ccgccttccg gcgcgggtgt aagagtgacg
    17341 ggataacgca taatttcgac tgtctctcgc atgtcattaa cctttttaat tggcgattaa
    17401 ctcaaaccga gttgtttcag gattgcttta cgcaggagtg acgcggcatg acactgcgcc
    17461 tcccatgaaa cctgagtttc aaatggttgc ggccattcgc tacatcgacg ccctgagatt
    17521 cgagccaacg tctgaactcg ctttgtttca catcgcctcc ctgccgctga gtatatcaac
    17581 aaagtaaaca ttagtgttta cctctgtgtg taatttgctc gaaaatagca ctaaatcgcc
    17641 gcaacggcag aatgcaatgc aaaatcagga aggaagattc cagaatttct ttgcgctaat
    17701 ttgcaaaact gaataataac ctcaagaaac gatcggcatc atagcggcaa aaggtaagaa
    17761 aaaggtggca ggatgtttac gaaggcgtta tcggttgtct tattaacgtg tgcgctgttc
    17821 tcaggacaac tcatggcagg gcacaaagga catgaatttg tgtgggtaaa gaatgtggat
    17881 catcagctgc gtcatgaagc ggacagcgat gaattgcgtg ctgtggcgga agagtcggcg
    17941 gaaggtttgc gcgagcattt ttactggcaa aaatcgcgca aaccagaagc gggacaacgt
    18001 tgagtcattt gttgccggat gcggcgtgac gccttacccg gcaggcaata tcatgcagat
    18061 tcaatgagtt gtgagtaacc agaaaactgg cgttttaccc ttttcgctta ggcaatgttt
    18121 tcagcagctc atccggactt acggatgcca ctacgcttcc cggtaacggc attttcagta
    18181 tgtgattttt gattttgcca atgacgtgca tctcgcatgg gcggcaatca aactttagcg
    18241 ttaatacttc atcgccgtta accagttgca ttggcgtcgc cttccagctt ttgatattgc
    18301 ctttcgcctg tttcgggcac agattaaagg caaagcgcag acaatgcttg gtgatcatca
    18361 ccgggacttc gcccttctct tcatgtgctt catacgccgc gtcaatcagc tgcacaccat
    18421 agcgatgata aaattcacgc gctttctggt tgtatacgtt cgcgaggaaa ctcagatgcg
    18481 tttgcggata aaccggcgca ggatcagcaa ccggtttacg gctgccgcgc tggtaactgg
    18541 caagacgcgc agcatccagc atgtcagcag cttcacggcg gaactggttt aacagactgt
    18601 ttggtacaaa cagcgccccc ggcaaattaa tttgcacatc gcgggcgtaa tagagggttt
    18661 gccccagttt tgccagacca tccttcagat tgttcattgc tttttcggcg ttattggctt
    18721 cgtcgaactg cccgtccagc gtatgcgtga tgctgacacc ctcttcactg gtgagggtca
    18781 gaatcagttg ttcctgccag ccgcccagtt caatgtctac cgccacccga cgttcgctgg
    18841 aggtttttgt cagtgcctgc tgccagttat gatcaagatt acggtttagt gggtgatgtg
    18901 gacgaatttt gtgcaaatct gctggcattt cattgggcca gacgcggtac tgattttctc
    18961 cggttttctc gaccgtattg gcacgaaaac cgacgacttc acgtttaatc aacacgttca
    19021 ggccatcgcc atttgccagt ggctcggtaa cggcaacatc gagatgatct ttcgccactt
    19081 tcaatacttc gcctaccggc aggccgataa atttcggcga atcgaacgcg ccaatatcgc
    19141 ctttacgggc attcacaaaa taatctgtgc taccacggtg gaaagtcttt tccgtcgatg
    19201 gaacaaagaa atgttcagta cgacctgatg aagcgcgcgc cagatcgcca cgttcttcaa
    19261 taatggcatc aagcatctgg cgataatggg cggtgatatt cttcacgtag ctcatatctt
    19321 tgtaacgccc ttcaatcttg aaggagcgta caccagcatc aatcagcgcg ccgaggttgg
    19381 cagtctgatc gttatctttc atcgacagca gatgtttttc ataggaaacc acccgcccct
    19441 gatcgtcttt caatgtgtat ggcaaacggc acgcctgcga gcaatcgcca cggttggcgc
    19501 tacgccctgt ttgcgcatga gaaatgtagc actgacccga ataggccacg cacagtgccc
    19561 catgaataaa gaattcaatg gtcgcgtccg tagcctggtg aatcgcgcgg atctgatcaa
    19621 gattcagctc tcgcgccagc acaatctgcg tgaagccaac atcagagagg aacttcgctt
    19681 tttcaactgt acgaatgtcg cactgcgtac tggcgtgcag ttcaatcggc ggaatatcaa
    19741 gttccagaat ccccatatcc tgaacaatca gcgcatcgac accggtctgg tagaggtcag
    19801 taatcagccg ttgcgcgggt tccagctcat catcatgcaa aatggtgtta agcgtgacga
    19861 aaatttttgc accataacga tgggcaaacg gcaccagctc ggcaatatct ttcaggctat
    19921 tactggcatt atgacgggca ccaaaaccag ggccgccgat ataaacagca tcggcaccgt
    19981 gcaaaatagc ttcgcgggca atggcggcat cgcgtgccgg gcttaacagt tcaagtcgat
    20041 gagaagatac ggtcacattc ttatccagta gctgaaaaat ggcggctatt ttagccacaa
    20101 gcgcgacgtt gcgaaatagt tttcccgctt aactgcgcgg gtaatggatg agggaatgaa
    20161 aatgtacggt ttgctcccca ccattacgat agatatgcgt gacgtctgca gcaaatcgaa
    20221 cgccttcccc acaattaagg gtctgccatt ccccatcaac gcacagatca agttgtccat
    20281 caatgacgac gacatgttcg attaccccct tctcatgggg cgtcgattcg cttattgcac
    20341 cgggtgccat ctgaatcgag aagtgttcaa agcagagttg tggatcataa ggaaacagcg
    20401 aagtgatcac catcgcctgc tgttgtggat cataaactga aggcgtagcg gactgtggcg
    20461 gagaaataaa tgtcgaaaat ggcacattca acccggtggc aattttccat aatgtcgcga
    20521 ccgtcgggct ggactcatta cgctcaatct gccctaacat tgctttagaa acgcccgtcg
    20581 cttctgccag tcgcgaaaga ctccagccgc gctgctggcg tagttgttta agtgtggtgg
    20641 ataaaaagcg agcgagattt tccatgcgac tcctccggca aaacggaagt ttatcacttg
    20701 tgcgttataa cggacaaatg ctacggtgcc tgtacgctat aacgtacgag gtgactatgc
    20761 gtctgttttc tattcctcca cccacgctac tggcggggtt tctggcggta ttaattggct
    20821 acgccagttc aggggcaata atctggcaag cagcgattgt cgccggagcc accactgcac
    20881 aaatctctgg ctggatgacg gcgctggggc tggcaatggg cgtcagtacg ctgactctga
    20941 cattatggta tcgcgtacct gttctcaccg catggtcaac gcctggcgcg gctttgttgg
    21001 tcaccggatt gcagggacta acacttaacg aagccatcgg cgtttttatt gtcaccaacg
    21061 cgttaatagt cctctgcggc ataacgggac tctttgctcg tctgatgcgc attattccgc
    21121 actcgcttgc ggcggcaatg cttgccggga ttttattacg ctttggttta caggcgtttg
    21181 ccagtctgga cggtcaattt acgttgtgtg gaagtatgtt gctggtatgg ctggcaacca
    21241 aggccgttgc gccgcgctat gcggtaattg ccgcgatgat tattgggatc gtgatcgtta
    21301 tcgcgcaagg tgacgttgtc acaactgatg ttgtctttaa acccgttctc cccacttata
    21361 ttacccctga tttttcgttt gctcacagcc tgagcgttgc actccccctt tttctggtga
    21421 cgatggcatc gcaaaacgca ccgggtatcg cagcaatgaa agcagccggg tattcggctc
    21481 ctgtttcgcc attaattgta tttactggat tgctggcact ggttttttcc cctttcggcg
    21541 tttattccgt cggtattgcg gcaatcaccg cggctatttg ccaaagcccg gaagcgcatc
    21601 cggataaaga tcaacgttgg ctggcagctg ccgttgcagg cattttttat ttgatcgcag
    21661 gtctgtttgg tagtgctatt accgggatga tggctgccct gcccgtaagt tggatccaga
    21721 tgctggcagg tctggcgctg ttaagtacca ttagcggcag tttgtatcag gcgctgcata
    21781 atgagcgtga gcgagacgcg gcggtggtgg catttctggt aacggcaagt ggattgacgc
    21841 tggtcgggat tggttctgcg ttctggggat taattgccgg aggcgtttgt tacgtggtgt
    21901 tgaatttaat cgctgacaga aaccgatatt gacatcctcc acgccctgaa ggacgtggat
    21961 ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgggcgg
    22021 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
    22081 gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt
    22141 gacaggcggt tctctttcgc tgtatgacca cagtacgcgc aacgctggct tgtatacgct
    22201 ggcggaacag caagcacctg accgccacgc cagagctgct tgtactcaag ctggcggcgc
    22261 atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg
    22321 cgccccggct ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc
    22381 tcaatgacta tcattgcgtg gtttttgctg acggccgttg tgactttgtg aaggtagtcc
    22441 ctgcggatat ttgcgataca ggaatgcagt cgctgtattt tgcgtttctg cttctgccag
    22501 ttgttgctga atttgacctt gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg
    22561 tttttctgaa aactgtttac aggctcaaag actgtgccat ctgacagcgt ggcgagttta
    22621 gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt ggatacttca
    22681 ctttctgtct gaatactgat gtaccactta ccgcaggact ggctgacagt gacatttttc
    22741 acaacacccg tgacctgacg gctgttccgg tagcgcatcc agcccagttt cggcagaaaa
    22801 atacggctgt tttcctgatc gagcttaacg ccctgcgggt agcggaatac atcattctgt
    22861 ccccgctttt tgaatcgggg aaaagcagcc cgattctgga agaagttttt gtaagcccgc
    22921 tcaaggtctt tcagtgactg ttgcaatggc tgtgacgggg catctttaag ccattgcgtt
    22981 tcagtggcat ttttccactc aaccagccag gaagccattt tcccgtaagg gatgtattta
    23041 ttaccgacct catgattctc attctgacgt gccagagcac gattgaaaac gaaacgacat
    23101 gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac cgggtcttaa ctggaattta
    23161 aatgcttgta gtcgtttcac aggccaaatg atactttggt ctgtgaaaaa agaaaccgat
    23221 atccggcgtg gaaggcattg tgttttcctg atgcatgttc acctggtctt tgtcaccaga
    23281 taccgacgtc agatttttga ccatgacgcg acagaaaaac tacgcactta cttttcaaat
    23341 gtatgtgctt attttgaagc tgaactggtt gaaatggatg gcgaaccaga tcacgtccat
    23401 ttgttaatta actaacctcc caaactggcg atatccagtc tggtaaacag cctcaaaggc
    23461 gtatcgggta ggttactgcg acgagatcga ccagatattg cagtcaggta ttactacaaa
    23521 ggcgttttgt ggagtcctgg ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc
    23581 atccgccaat acattgaaca acagcaaaca cctggtcagg tggaaaaccg cgccttatat
    23641 ccccgccctg aaggatgggg ttttacggcg caccggataa aaagcgccat ctaacgatgg
    23701 cgcgtctgga actacttttt gttaacgtca aacatggtgg catcggtcgc catgtcatca
    23761 ataacttgtt taatattttc gaaagccatt ggcgtacttt cgttattcag gtctttacct
    23821 tcgccctgac gaacgacttt gataaccggt ttattagtcg ctgcatcaat cagctcacct
    23881 tcaaaataga ggcgagtatc catggtacgg tggcctgtag ccatttgcgt ccccgcaacc
    23941 actaatgcaa caggaaccac ttcatagaat tgcagccctt ctttgctggt atctacaccg
    24001 gtaatggccc cacggaaaat cagactacgc ggcccagcgg tggtaaccac tggtttacgc
    24061 tgcgctatcg cttctttcat ttcggtgttg gtatagttca aaattttatc cagaactttc
    24121 tgccctacct gggtagaagg ttttggtacc ggataataag tgattgggtt ccagacgatg
    24181 ctgtcatatt tgctttgatc aaaactcggg tctacccaac gtaaaacagg tttacctgtt
    24241 gctgaggttg tttcttttaa atcagagtaa ttgtttaaaa aaccagaata tttatctggc
    24301 tgggtgattt tagaggcaca accagacaga gccaacaagc cgcataaagc tgcaactttc
    24361 gcaaatgatg tggtacgcat gaatagtttc ctgagatgat tatattactt tctaagttat
    24421 agcaaaaagc gccgcttcgt gttgtggcaa aaaaagtgtt ggtgaaaata aatttttcag
    24481 ccccttttca gacgacatag tcaatgccga aaaaggggct aagtcaatca tcagtagtta
    24541 aatttcagta agttaagctg ctaaaaactt cgactactgt gattaatttc acatcccttc
    24601 gataaaaaat atgcttaccg aaaagaagaa cagtaaatca tctcagcgtg gggaattttc
    24661 acgcgttttg tgcttaccgg gtcatttttt acgtgccagc atcgtggcga aacgcagttt
    24721 aatacgatta ccgttggcgt cggtgcggtg cagctcgccg acgtcttcat tgtatttcac
    24781 cctctcccag ccttcataat atcgacgtaa ttctccctct ttgaaggcaa acgggaagcc
    24841 gacggtacat ggataatcag cggtatccat cgccgccaca atcaggttgt aaccaccagg
    24901 tttagtgcaa cgttgcatat tggcaatcag ccctgggatg gttttagcct caaggaacat
    24961 cagtaccaca gtcgaaagaa taaaatcgta ctgtctatca aatgtgaggt tattcagatc
    25021 aacgacccgg gtgtgtaaat tatccagatt ttcaatggat ttaatgcgct ctacattggc
    25081 gatactcata gcatttttat cccatgcgtc aacatcataa ccattggctg ccaggtaaag
    25141 actgttacga ccattgccac agcccagatc cagcgtttta cccggtttaa ccactttcac
    25201 cgcttccagt acttcagagt gtgtgcgggt taattcatat ttatcagtaa aatagttttc
    25261 gtcacgaatg atcattcttt gtcctctgct ttcattaaaa cggcgcgctc ggttctgact
    25321 aacaattttc cctgcatcag aagcgcaaaa gtacggatga gcagtattgc aataataaaa
    25381 ttggtaaaga taaacagcgg taccgccagc gtatggaaaa atccattttc gctgccactc
    25441 cccagatgca aaccggtggt tgccagtgca gatacgccga acgagaaact ccagaatgaa
    25501 gcattaaatg gctgggagag ataccatggc atcagacgta gcataaacag cagttgcagc
    25561 agtccataac caaaaagcat tttcgccagc gtatcaccct cgccgccgtt gacgctcaac
    25621 caggcactac aggccaccag cgcaggagcg agctgaatgc cgagtgatgt ccgcagtgcc
    25681 gtgggtaatt ctcccgaact gcgcagacgc tgcaagatca caggttcaag gcttagccat
    25741 gagaaaacgc ctgcgcctaa aaacaccaga ccggcgtcgg tgtagcccaa cgcaccacag
    25801 gccattgcgc tgataaagtt gttggcaact gtcggcagat acagtccagg cgtggtagct
    25861 tcttcagggt gagatccgcg ccataatccc gcagtttgcc aggcggcata agccaactga
    25921 accacgacac caaaactgaa caggcacacc gccagtgggc gaaaccacgg aacaaaacca
    25981 atcgccacca gcatcgtcgt tgccggaaac aaactcacaa aactgctcat cactggatgg
    26041 cgcacttctg ccagcacgct atgcggaaag cgtataagtc gggtaataaa tgcgctagtt
    26101 aacaatcccc agatgatcat cgccagaatc accagcccat cccctaacca gtggctgacc
    26161 tgccaaacct ggctggcata gcgccaggca aatcccatcc ctatcgtccc caacacaata
    26221 ccaaagtagc ctgccggcaa attgagcact ttatcgctct gcatcttctt actcattttg
    26281 tttaatttat aaagtatatt tgaaatgcat tttatggaat tttctcgagc atagccagag
    26341 ccgcagaatt tgctacggtt atagtaagtc acctgtggga aaacgctatg cgcaaatatc
    26401 gtctaagtga agaacagcga gcctttagtt atcaggaaga tggcactaaa aaaaatgtgt
    26461 tattacggca gattatcgcc attagcgatt ttaacgatgt gatagcagga accgctggcg
    26521 gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa ttgctggatt tacgaccaga
    26581 acgctattgc gttcggcgga gcggtgattt ccggtaatac gcgcatcacc ggcaccagcg
    26641 tgctgtgggg agaggtttac gcaacggata atgtctggat cgataatagt gaaatcagcc
    26701 agggtgcata tataagtgac agcgtcacca ttcatgactc actggtttgc gggcaatgta
    26761 gaatatttgg tcacgcctta attgatcaac attctatgat cgtcgccgca caaggcttaa
    26821 cgcccgacca tcaactcctg ctccaaattt atgatcgcgc aagggttagc gcctcgcgta
    26881 ttgtccacca ggcacaaatt tatggcgatg ccgtcgtccg gtatgctttt attgaacatc
    26941 gcgccgaagt ttttgatttt gccagtgttg aaggtaatga agagaacaac gtctggctat
    27001 gcgattgcgc taaagtctac ggtcacgcgc aggtgaaagc tggcatagaa gaagatgcca
    27061 ttccgacgat tcattacagt tcgcaggtgg cggaatatgc cattgtggaa ggtaattgtg
    27121 tgttaaaaca tcatgtcttg attggcggaa atgccgtggt acgtggtgga ccgattctgc
    27181 tcgacgagca cgttgtaata caaggtgaga gccgtatttc aggtgcggtg ataattgaaa
    27241 accatgtgga gttaaccgac cacgccgtcg ttgaagcctt tgatggcgac acggttcatg
    27301 tccgtggtcc aaaagtcatt aacggcgagg agcgcattac gcgaacgccg ctggcagggt
    27361 tattatgaat cgataatacg cgcgtataag ttcacatcat cataggcatc attcaggaac
    27421 tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc gcaaagcgac ctggttgctt
    27481 tgcggattgt ccacccgaca tttgatcacg aagcgtctaa gttcaccaga ctgagcgtaa
    27541 tgatgaatca atgcctgcag cgcctgagaa atgatcccct gcccctgatg agattcgtcc
    27601 agccagtagc cgatttcagc agttttactc agtggttcaa tacgattaaa cgagataacg
    27661 ccgataagtt catcttcttt gaaaatcatg aacattttgg catagccgcg ttgatgcaac
    27721 atcacattac cctgcaccgt ttttcgcgtg tcctcttcac tctgaacgaa ctgcggccag
    27781 tttagtgact gctgtaacca ggttttattt ttacagatta actgataaag aggtgtgacg
    27841 tgactttccg caacagcatg taattcaagt gattcgctta cttttatcgt ttcagtcatc
    27901 ttatctccag tcaatccatg ctgttgttaa aaaagccgcc tggctggcgg cttaggaaag
    27961 tttacacctc tttgacgctc tcctgctgca ggattttgag catctcatct tttaactgta
    28021 gcttttgttt tttcatgcgg accacttccg cattgtaccc tcgaccgtcg gaaccttcct
    28081 ttctggcaat ttcatgatca agtttattgt gtttatcgaa caaggacata aagcgaggat
    28141 tttcgttttt cagacgggat attaaatctc ggtattctgg aaacatacgc actccctgtt
    28201 agtggctgac aaggtgtatc tgaaatacac accatcagat tactcattta acttagggaa
    28261 aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc gaagaaggtg ccgaagaaaa
    28321 aacgattaac tcatcacgcg gtcgtcaaca tactgacgtt tatccggcgc tggcgggaaa
    28381 tactgataca gccatgtttc actgatagcg tcgccctgac aacgcagata catccgcata
    28441 tcgaccggat cagtggagtc cgaagtcgga taccagtcaa actgaatacg ataaccatca
    28501 atgggttcaa tatagagaat ttcgatttgc ttcgcttccc cagtggaaag cgtaatcacc
    28561 ggctcaatgc cttttggtgc ggcagctttc agatcaccac caacgaaatc gacggcaaaa
    28621 cgacgcgccc atttttcggg atagtgttca cctggtgccc aaccttccgg gaaaccgccc
    28681 atgccggtac gcgtcgccat aacgcgcgct aatgggcaat gaacaggcgg ttgcgcactc
    28741 cagtacagac gatactggaa tgcaaactca tcacccgcct ttacagcttt ttctggctgc
    28801 cagaagcaga caatgttatc cagcgtttcg cccgttgttg ggatttccat caggccgatg
    28861 gtgcccttac cccacttgtt acgcggttcc acccacagac ttgggcgttt gttataccag
    28921 cccataatgt cctgataatg ggagaaatca cgatccagtt gcaataaacc aaaccctttc
    28981 gggttgttgt cggtgtaagc attgaactgc aatttttgcg gattattcag cggacggcaa
    29041 atccactcgc cgttgccccg ccacatggac agacgatcag agtcatgaat ttgcggatga
    29101 atggtgtcgc acatccgacg ttcattagta ccgcagctga acatactggt catcggcgca
    29161 atgcccagct gtttaatgtc tttgcgcgca tacaggtgat tttccacatc cataatcacc
    29221 tgacttttct cacaatggat agtgaactta taggcaccag taatgctggc gctatcgagc
    29281 aacgcataaa cggtaaaggt agttgccccc ggttttaccg tatcaaacca gaaggcggta
    29341 aagtcgggga actcttcttt actgtcggtg taagtgtcaa tcgccaggcc gcgggccgac
    29401 aaaccgtatt gatatgtgtc atcaacggcg cggaaataac tcgctccgag gaatgatact
    29461 acatcacggc gcgccagttc gggggcttta aacacgcgaa aaccggcaaa gccgagatcg
    29521 ctttgccctt ctaattgttt ggtatcaaca cctgcatcgt tgtatttgaa caactccggg
    29581 cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta cagaaaacat acgaacgcgg
    29641 cgacggaatc ccattcccat atggaagaac tgagcgtcca gttgacggtt ctcaacgtta
    29701 tgccagagcg atttttcggc gtcgtattga atactgttat aagcctgcgg cgtcattgtc
    29761 gccagcgtgt caggtaacgg acgaggcgca ccacgccacg ctgtttgcgc taagtcgtgc
    29821 gccattgact gtagaatgga gaagtcaaaa cgctgggttt gcccgtcggc aatatcagaa
    29881 tctgccgcga atgccgcctg agaaaaaaga gaagcaatgc cgctggtacc gcacacggcg
    29941 gccatagcca ttgaaccttt aataaatcgt ctacgatcca tacctgaaag tgagtccttc
    30001 tggtgttgtg aatggtcccg cgctggtcgt tttcagcggc aagaaagtgc ttatttatga
    30061 ccgctcactc tagacaatat ttattaataa tccaattgtt gaactcagag aatatgcata
    30121 aatcggcaat ttttttatgt cgatgggatg agcctgattc gtttgtaaaa tcaggcaaat
    30181 ttttgcaaag gcggatggtc agaatttaaa gcaaaccaca gcgacgttta ctgcgttctt
    30241 ccgcttcctg ataaagctgg aactcatcca aaaccggaca acccaacgcc tgctcaaatg
    30301 cctcgcgact ggcattaccg tgattgcgtg cctgcgtttc attacccaga tttgactgga
    30361 agatccccgc cgcgctaacg ggcaagaaat cttcataggt aatgggttgc gccgcgaccc
    30421 aaccacgttc aattaagggc tgtggatcgt ctccgggatg aatcgcctga cgatgcgcct
    30481 cacctgaagg cgtcagacgg taccggaacc atgccagccc ttgctgacgc attaaaaact
    30541 cactgtcagg aaaagtgcgg aaggtttcct gtaaatgcat ttggtgagtg agattatcct
    30601 gcccggttcc agcgttacgc agaagatcat catacagttg tcgccctttc ggcgttaatg
    30661 ccacgccacg ctgctcaatt tcaccaaagc gcgcggtatg cgtgccctgt ttctgccccg
    30721 caaacaacac cgtctcttcc agtgctttaa agctggtctg gcgtagtaaa atcggtacct
    30781 cgcggcgcgg cggcccctcg atgagaattt tgggttcaat tccgcattca ggcatcatcg
    30841 actgcacccg gtcaatatcc agcgtacgtg gcgtcaggtg gttgatatgg catccaggaa
    30901 aacagaccac atcagcaatc aaccgatgtt cgttgtgcaa ggcgcgatag gtttcttcat
    30961 ctaccgttgc tgactggtgc cagcgaaacg tttccagggc ttcctgcaca aactcctgtg
    31021 cctgtgtttc gttaaaaccg ccctgctgct catattcctc taacagttgt cgacaacgtg
    31081 gggtgaagat atcgcgctga cgcagaatct ccgccgcttt ctggcgcaaa atttcgttct
    31141 cgataagctc aaggcggagt aaggaggtaa aaacgcggaa gggattacgc gccagagaag
    31201 catcatcaat gggccgaaat gctgtcgaat gtaccggcac ccctgcctgc gagagatcgt
    31261 aatagctcac cgggtacatc cccataatgg caaacatccg ccgaagagta gcaagctctt
    31321 gtgcagtccc aacgcgaatc gccccatgac gttcaacatt cagtcgcgcc agctcgtctg
    31381 catttaccat tttttcgtgc agttgaggat tgttttccag cacagccaga ttcacatcag
    31441 ctaccagttc cagcagcgtg ccatattgcg gaacttcttg ctggtacatg gctgacattg
    31501 cctgcgaaaa ctgttcccga atctcatccg ccgtgatgct gttcgccata gtgtctgcct
    31561 ccagtggtca gtaatctgga gtgtaggtaa ccgcattcac tctggcggga agaatttaca
    31621 aactgtgatc tcgccgcgaa aacatcaata ttatccattt tgctgtaaca taattgcctt
    31681 aattgttaat aatattttgc aatcaagtta tcataatcaa acaacttcac ttgtcagcga
    31741 caccgcttcg tttttaacat cgcttatgga aaaaaatagt ctgtttagtc agcgcatccg
    31801 tttgcgccac cttcatacat tcgtagctgt cgcacaacaa ggaactttgg ggcgcgcggc
    31861 tgaaaccctt aatttgagtc aacctgcgct ctctaagaca ttgaatgaac tggagcagct
    31921 gactggcgct cgcttgtttg agcgtggtcg tcagggggcg caacttacct tacccggcga
    31981 acaattttta acgcatgcag tcagagttct tgacgccatc aacactgccg gacagtcgct
    32041 tcatcgtaaa gaagatctta ataatgatgt cgtcagggtt ggtgcactac ctactgcggc
    32101 actggggata ttaccttcgg ttataggtca gtttcatcag caacaaaaag agacgacctt
    32161 gcaagttgcg acaatgagta accctatgat tctggcgggt ttaaaaaccg gggaaatcga
    32221 tatcggcatt ggtcggatgt cagatcctga actgatgacc gggcttaatt acgaactgct
    32281 gtttcttgaa tcgctgaagc tggttgtccg ccctaatcac ccgctacttc aggagaacgt
    32341 aacgctaagc cgggtgctgg aatggccggt cgttgtatca ccagaaggca ctgcgccacg
    32401 ccagcattca gatgcattag tgcagagcca gggctgtaaa attccttcgg gttgtatcga
    32461 aacgctgtct gcttcgctat ctcgtcaact tacggttgaa tacgactacg tgtggtttgt
    32521 cccttctggc gcggtaaaag acgacctgcg tcatgccacg ctggtggccc tgcctgttcc
    32581 gggacatggt gcaggcgaac cgattggaat actgacccgc gtagatgcga cgttctcttc
    32641 tggttgccag ttgatgatta acgctattcg aaaatcaatg ccgttctgaa aggtgaaggg
    32701 atctgtcgat ccctccttga acattttcac accgtagcga aactaactgg ttcacccgct
    32761 ccgcgaggtt ctgccgacac agaatgtttg ttcagacgga atacgtccac cgcctcagtt
    32821 aatcgtgccg cctgttcttc aagggacacc gccgctgctg aggcctcttc taccagagaa
    32881 gcattctgtt gcgtcacctt atccatttca gaaatcgcct ggctaacctg agttatgcct
    32941 ctactttgtt catccgaggc ggcggcgatt tcctgcatga tatgtgtgac actcgcgacg
    33001 gcatcaacaa tagtgctcat ggttttccct gccgttgcca cctcatccga ccccaggtca
    33061 attaacctga ctgattcact gatcaagcct tcaatctctt tcgccgcttg ggcgctgcgg
    33121 cttgcgagtg tccgtacttc gctggcgaca acggcaaatc cacggccttg ctcacccgct
    33181 cgcgcggctt caacggcagc attcagtgcc agaatattcg tctggaaagc aatactgttg
    33241 atgacggcgg tgatctcgga aattttcttc gaacttgtag agatagcgcc catcgttttt
    33301 actacaccgg aaaccgtctg cccgccatcg ctggctttaa tagaagcctc ttgcgccagt
    33361 ttgctggcat gatgcgcgtt atccgcattc tgtttcaccg tcgcagtgag ttgctccatg
    33421 ctggcggcag tttgttcgat agccgccgct tgttcttcgg tgcgagatga caggtccgca
    33481 ttgccagctg aaatttcgct ggtgccacga taaatctctt ccgcgccctg tcgaacagtc
    33541 cctactgtca tccccagtga atgctgcatt tgctgtaaat gacgacttaa gcgaccgatt
    33601 tcattacgac ccgcaggttc atcattcatc gtcagatcgc cactggcgat tttttcaatc
    33661 cgttgtgcgg catgttgcag tggacgaatg acgatccgac gtagcaccat aaatgttatc
    33721 agcgtcatga ccagggcaag cacaaacgcg ccaatcatga acatcccacc caggcgggtg
    33781 cgctgatggg ccagttccgc cagttggttg gctctggtgc tacgtatctt aacggctttg
    33841 ttcaaaatat cggtataagc attatccagc ggtcggatct gctcactttc atgattgata
    33901 atcgcttcaa acatgccatt tttggcatat ttcaacatag gttgcatacc cgtgatataa
    33961 gcctgaaagc gttgatttaa ttcagtgtcg agggcttcat cagcaggtgt tttcaccggc
    34021 cgattctgat aagcacgata accttgctgc gactgtttaa tctccgattc ggcttgcgca
    34081 atattgcgct tcattgcttc catttccgca atacgactcg cagccccggc ctgaatcata
    34141 ttgatccggg ctgaacgcaa aaaatctgaa ctgttcgcta acccggtccg aatctcaatc
    34201 tccgcagtaa catcacgctg atcgcgatcg gcctgccaga ggaaatagcc agccagagca
    34261 gaacttaatg cgaatagaac taagataccg ccaagaatgc aggcaaataa cggaaccagc
    34321 ctgatgtgat gcaaaaaacc taatcgatgt gagggagttg tattcattac cgttgtctct
    34381 cgtccaggtt tactcctgat agaaagtcat cggcacttga cggtaattac ttatgaccaa
    34441 aaacctcttt gcgtcacatt tttacaacaa ttattgcaga gaaaattaat gctattggaa
    34501 tgaagagagt aacgaatggc taaactggct aacccgaatg acgaaaaatt ctggaaaaca
    34561 gcacgcaatt tcgtgaagtg attattttcg tccctaagga aacctcatag gatgcctgcg
    34621 ggtgcctgag actttctgtt ttcaggctaa ccgggaatca gaaagacgaa ggccccgacc
    34681 gatatttcta tcaaccgagg ccaacgaatg aacctagcaa ttcagatctt agcctcttac
    34741 ccgccatccg gcaaggagaa aggctatgaa gcacaaccct ctggtggtgt gtctgctcat
    34801 tatctgcatt acgattctga cattcacact cctgacccga caaacgctct acgaactgcg
    34861 gttccgggac ggtgataagg aggttgctgc gctcatggcc tgcacgtcca ggtaagggca
    34921 agcgcgggga ttttccccgc gcatctgact agcagattca ttttctcatg gcaccccttt
    34981 tacacttcag ttagtgcatg tttttttcga ggcactaata gaaccatcat tacagacaaa
    35041 tttgccgcct gccgtacagt gtgagatccc ccctttcgaa ccagaacagg gtttgcgccc
    35101 ggcatcggca taaaaggaaa caagcgttcc cataaacaaa ataagtgcca gttttttcat
    35161 ataacctcca tggtttatcg aatcttatgt tcgatttgaa cgttatacgc tggtttattt
    35221 tgataccgtt gatgagatca aagatcgggt gaattgatta aaaataagcc agatggacgt
    35281 atgggcaagg attatggttt tatttgtcat acaaataaat ataataggcg cttccgatgt
    35341 agacccgtat tcttcgcctg taccacgggt cggttttagt acaggcgttt tctttaaata
    35401 tccttcagga acgtttacgc ggcatcatgc gtagaagtgt gttgtccttc cagaaatagt
    35461 ggtgtgccag tgcagccgca gcgtgcaacc cgatgacaaa atatcccaga ttcgccagcg
    35521 tctcatgcca cgactttaag ctatcaaccc gttcgaaatt ggcctctgaa gcgtaaggca
    35581 tcgtcaaacc aaacgcaaac cacgggttgc cccggttata catcatcacc aaaccaatca
    35641 ctggcagcgc aataaacagg agataaatca ccaaatgtcc caaatgcgcc agtcccgtca
    35701 tcatcggttt tggcttaggt ataatcggcg gggttgggta tttcagtctt aacaaaagac
    35761 gaaccaccat cagcacgaga attgagatac cacaggaaac atgaaccatg ttgatgagtg
    35821 gccgatcgct acgtgggaag aaaccacgaa actccattgc gcaatacgct gcgataacca
    35881 gcaaaaagac cagccagtga atgctgattt gtaaccttga gtatttattt tccataacat
    35941 ttcctgcttt aacataattt tccgttaaca taacgggctt ttctcaaaat ttcattaaat
    36001 attgttcacc cgttttcagt acgttacgtt tcactgtaag aatggagatt gcccatccca
    36061 caatcctggt ctaagcctgg aaaggatcaa ttttcatccg aatgttcctg acaggagaaa
    36121 accatgagta aagttggtat taacggtttt ggtcgtatcg gtcgactggt gttgcgtcga
    36181 ttacttgaag tcaaaagcaa catagacgtt gtcgctatta atgatctcac ttccccaaaa
    36241 attctcgcct acctgctgaa acatgattca aactacggac cattcccctg gagcgttgat
    36301 tttacggaag attcacttat cgttgatggg aaaagtatcg cggtttacgc cgaaaaagag
    36361 gctaaaaata ttccgtggaa agcgaaaggt gcagaaatca ttgtcgaatg tactggcttt
    36421 tatacctccg ccgagaaatc gcaggcgcat cttgatgctg gtgcgaagaa ggtgttgatt
    36481 tccgcccctg ccggtgaaat gaaaactatc gtttataacg tcaatgacga cactctggat
    36541 ggcaacgaca ccattgtttc cgtggcgtca tgcaccacta actgtcttgc gccgatggcc
    36601 aaagccttgc atgacagttt cgggatagaa gtcggcacga tgacaaccat tcatgcctat
    36661 actggcaccc agtcactggt ggatggccca cgtggtaaag atttacgtgc ttcacgcgca
    36721 gcggcagaaa atatcattcc ccacactacg ggggcggcaa aagccattgg tctggtgatc
    36781 ccggaactga gcggcaaact gaaaggtcat gcgcaacgcg tgccggtgaa aacaggttcg
    36841 gtcactgagc tggtgtccat tctcggaaaa aaagtgactg ccgaagaggt gaataacgca
    36901 cttaaacaag caaccaccaa taacgagtca tttggttata ccgatgaaga aatagtctct
    36961 tccgatatca ttggcagcca tttcggttcg gtgtttgatg ccacgcaaac ggaaattacc
    37021 gccgtgggcg atttacaact ggtgaaaacg gtcgcctggt acgataacga atatggcttc
    37081 gtcacgcagc ttattcgcac cctcgaaaaa ttcgctaaac tctgacgcgc acaggcggag
    37141 gaaaaaacct ccgcctcttt tactcattac gactgtaaat aaaccacctg ggtctgcaga
    37201 tattcatgca ggccatgttt accatctgcg ccgccaatac cggatttacg ccatccggcg
    37261 tggaagcctt gcatagcttc gaagttttca cggttgatgt aagtttcacc aaacttcagc
    37321 cctttaatgg ctttcatcgc gacgttcaga ttttgggtat agattgatga ggtcaggccg
    37381 taatcactgt cattagccat tgagatagca tcttccagcg tgtcaaatgc gaccaccggc
    37441 agaaccgggc caaaggtttc ctcatgcata atcgacattt cctggcgaac atccagcagc
    37501 aatgtcggcg gataataata tcctttcccg tctactgctt tgccacccaa cgccactctc
    37561 gccccttctt ctactgcgcg cgccactttt tgctcgaccc tttccagcgc cgcggcgtta
    37621 atcaacggcc ccatcgcaat gtcgttgcgt tcagcggggt taccaaattg aaccgcctgc
    37681 atcgcttcac ccagccgatt gacgaactga tcataaatgc ctttctgtac ataaacacgt
    37741 tctgcacagt tacacacttg cccactatta atgacgcgtg aatcaacgat ggctttgact
    37801 gccagttcaa gatcggcatc gtccattacg atagctggtg ctttaccacc caattccagg
    37861 cacactttgg tgatgttttt cgccgcagtc gccataatct tctcacctgc agagacgctg
    37921 cctgtcatac tgaccattgc gacctttggg ttacccgcca gttcttgccc aacggtttca
    37981 ccacgcccca gtacaaggtt aaacacgccg cgcggaaggc ctatttcatc gacgattttg
    38041 gcgaatgcaa tcgcattgtt tggcgtaaat tcactgggtt taatgacgat ggtattaccg
    38101 gtcaaaagag cgggagccat tttgcgggca atgaggaaga acgggaagtt ccacggcaga
    38161 atgccggtag tcacaccaag cgcacgttta aacaaaagaa tattttctcc tggacgatcg
    38221 ctttgaataa tctcgccctc gtaacgccgt gcccactccg ccatgtaatc gatatagtcg
    38281 gcagtaaaag ccacttcgac ttcagccagc tgctggatct tgcccccctc ttcaacaatc
    38341 agcgcactga tttcactggc gcgttcgcgg atgccggcgg agattttgcg caaccaactg
    38401 gcgcgttcaa tagcaggcaa cgcttcccat tctggttgtg cacgttctgc tgcatcgatt
    38461 gccttacggg catcctcggc ctgaccatcg ggtatgcggg aaatgacagc ctctgtagca
    38521 gggtttacca catcaatcca tgcgtctcca cgccaggtaa caaactgtcc atcgatatac
    38581 ataggatgtt gaacgggtac tgacatgggc gactcctgtg atttatatgt ttgttttcgg
    38641 tgaatgcatt gttaattaac atcaatcatc agagagacgg catcgtgaaa ggacaattct
    38701 gtgaagggct tcataaaaac aagtgtaaaa ggaataaaag gcagaaataa aagtcaggag
    38761 tcatgcccgt ttgcaaacaa gtggttcaca aacgggcaat ttttaacgta acgagcgact
    38821 ttccatcgcc tcgatgagca ccgcatcatg tttcagcgtt tgccatgcat ggatgacttc
    38881 tgccggaaaa tcaacgtgaa cgataaaatc tcgcccgcgg ggaccgtagc catcgctatc
    38941 atcgcgtaaa cgcggcagct cgccagtgag tagtgagaga taacgctcaa ccggccataa
    39001 tccttgtaac tgattaataa agattacact gtctgcgtta tttcctaact gaggaacgaa
    39061 tccgggataa cttaaccagc gtggtgcatc gggattgtcc ccagtcatac ggcggaacgt
    39121 cgccatcgtg cgccgctgca tggtggggtc ctgaacaacg atagccgtat gaactcgttc
    39181 tacggcctga ttcaatagcg cgatgctaaa gcgtgcgttt tcaccgcagt ttgttgactg
    39241 gtcttcaatc cagatttttt catgcggaat gtgccagaac tgatgagcga tatccgccag
    39301 gatggtcgct tctgctctgc cagtggtgcg gatagtgttg tagtgcggat gctgtgcgat
    39361 ggcgctatac aaaaaagttg tcgagtgacc gataccacca ctaatcagta aaggaatttg
    39421 ctgatcgcga gcaatcttac atgccgcgtc gatagtcggc ataaccgcat tgcctgcaag
    39481 gatcacgcaa tcggcctgat acggcacctc accggagaaa tcatcctgcg ccagccactg
    39541 tccgataaca tttatggcat ctatagttgc cggcgaaagc gtcggaaacg gggtaatgtt
    39601 catggttcct ccttatcgtt cccttaagaa tactgcgtga gataatagtg agaagcggag
    39661 cactctgaaa ggaggaagtc gtatgtcctt aaccgccact ccgcaagcct gacatatctt
    39721 ttaaggaaat gctgtagata aacattattc atcttgcagc agagaattgt atttttctga
    39781 attcctgaat ttttaccctt accgttttag aattaataaa aaacccggcg ctaaatgctt
    39841 accgggtttt ttctttatca ggcaaatagc agggttaacc gctaatctgc tccatcgcct
    39901 gcaaaatacg cttatctgaa atcggataag gcgtaccaag ttgttgagcg aagtaactaa
    39961 cgcgcaactc ttctatcatc caacggatct ctttcacggc ttcatcctca cgacgtgcgg
    40021 gcggcagttt gttgatccat tgctgccacg cctgctggac gttttcgact ttcagcatct
    40081 gagcacggtc gcgatgtgga tcaaccgcca gtttttccag ccgtttttca atcgcctgca
    40141 aatatcgcag cgtgtcgccc agccgtttga agccgttacc agtgacaaaa ccgcgatata
    40201 ccaacccgcc catctgcgct ttaatgtcag aaagccccag tgccatggtc atatccaccc
    40261 gccctttcag acgtttgttg atattgaaca ccgcagtgag gatttgctcg acctgcttcg
    40321 caatatccac caccgtgtca ttcagttcgg cgcgcacttt ttcatgcagc gcagcaaagc
    40381 cttcttccgt ccagaccggg ccaccattgg cgtcgatcaa ttgatccaca ccgcaggaga
    40441 tacagtcgtc gatcagctcc agcactttgc catacgggtt aaagtacagt cccagcttgg
    40501 ctttgttcgg taacttttca tgtaaatatt tgattggcga tggaatattc agcagcagta
    40561 gacggcgaag accgttccac attgcctgct tttgctccag cgggttatca aacagtttga
    40621 tcgccacact gtcgcgctca tccaccagcg ccggccacgc cttcactttg tagttgccac
    40681 gcttctgttc gtagctttcc ggcagctgac caaaactcca gatatgtaag ccgctctgct
    40741 cgataccgtc atccgccacc gcagatagcg tttcctgcac tttgcctttc agcgcatctt
    40801 tcagatcttg tagcgagcgc ccttctttta gcttcttgtt tttgtcatcc accacgcgga
    40861 aggtaatttt caggtgatca ggcacctgat cccagtgcca gtcttcgcgg tcaacggtaa
    40921 cgccggtcat ccgccgtaac tcgcgctcaa ggctgtcgag caacggtaac tccagcggtt
    40981 tgacgcggcc taaaaacgct tcggcatagt ttggcgcggg tacaaaatta cggcgtaccg
    41041 gtttcggcaa cgatttaatc agagcaatca ccagttcgcg gcgcagaccg gggatctgcc
    41101 attcaaaccc gctttcctca acctggttaa gtaacggcag cggaatatgt acggtcacac
    41161 cgtcagcatc cgcgccgggc tcaaactgat agctcaaacg cagcttgaga ttgccctgat
    41221 gccagaagtt cgggtaatcc agcttgctga ttttttctgc gccctctttg atcaacatgc
    41281 ttttttcaaa gttgagcaaa tcaggggttt cgcggctgac ttttttccac cagctgtcga
    41341 agtgacgagc ggagattaca tcgtggctga tgcgctggtc gtagaactca aacaacgttt
    41401 cgtcatcaac cagaatatcg cggcgacgtg atttgtgttc cagttcttcc acttcagccc
    41461 gcagtttcag gttttcacgg aagaatgcgt gacgcgtctg ccagtcacct tccaccagcg
    41521 cgtggcgaat aaagagttca cgacataacg ccggatcgat ctggctgtag ttgaccttgc
    41581 gcgcggcaac aatcggcaac ccgtaaacgg tgactttttc cgttgccatc accgcgccct
    41641 gcgcccgttc ccagtgcggt tcgctgtagg tgcgtttaat caaatgctga gcaactggct
    41701 ccacccattc tggatcgatg cgcgccgcaa tgcgccccca caggcggctg gtttctacca
    41761 gttccgccac cattacccat ttcggcggct ttttgaataa accagaaccg gggaagatgg
    41821 agaaacgcgc gttacgtgca ccggtatatt cctgtttatc ggcatctttc atgccgatat
    41881 gggaaagcaa accggtcagt aaggcgatgt gaatttcgcg atactccgcc ggttcgctgt
    41941 tgaccggaat gccaagttct ttcaccacct ggcgcaactg ggtgtagata tcctgccatt
    42001 cgcgcacgcg cagatagttg agataatcgg tacgacacag tcgacggaag gcgttagaag
    42061 aaagcgcctt ttgctgctcg ccaagataat tccacagatt cacaaacgcg aggaagtcag
    42121 actctttgtc gtggaagcga cgatgttttt cgtccgatgc ctgctgcttg tccatcggac
    42181 gctcgcgcgg atcctgaatg gagagcgcgg acgtgataat catcgcctca cgcacgcagc
    42241 catgtttttg cgcttccagc accatacgcg ccagacgtgg gtcgacaggc aactgcgaga
    42301 gctggcgacc gagcggcgtc agtttatagg cgctggcctg ttcatccgtg gtgatcgcgc
    42361 ccagttcttc gagcagacgc acgccatcct ggatattgcg tttatccggt gcttcgacaa
    42421 acgggaacgc agcgatatcg cccagcccca gcgcggtcat ctgcaaaata accgaggcca
    42481 ggttggtacg cagaatctcc ggatcggtaa actccgggcg cgagaggaaa tcgtcttcgg
    42541 aatagagacg aatacagatc ccttcggaca cacgaccaca gcgtccttta cgctgattgg
    42601 ctgaagcctg tgaaatcggc tcaatcggca aacgctgcac tttggtgcga tagctgtagc
    42661 ggctgatacg cgctgtaccg gggtcgatga cgtatttaat ccccggtacg gtcagcgacg
    42721 tttccgcgac gttggtcgcc agcacaatgc gccgtccgct gtgcgactgg aacacgcggt
    42781 tctgttcgct gttcgaaagc cgcgcataaa gcggcaagat ttcggtatgg cgtaagttca
    42841 gcttgttcag cgcatcggcg gtatcgcgaa tttcccgctc gccgctcata aagatcagaa
    42901 tgtcgccggg gctttcctgg cttagttcgt ctacggcgtc aaaaatcgcc tgcaactgat
    42961 cgcgctcggt gtcatcggct tcttcaacaa tcgggcgata gcgcacttcc accggatagg
    43021 tccgaccgga gacttcaata atcggcgcat tattaaagtg gcgcgaaaag cgttccgggt
    43081 cgatagtcgc ggaagtgata atgattttta ggtcaggacg ccgcggcagc aactctttca
    43141 aatagccgag cagaaaatcg atattcaggc tgcgttcgtg cgcttcgtca ataatgatgg
    43201 tgtcgtactg catcagcagg cggtcttgct ggatctccgc cagcaggatc ccgtcggtca
    43261 tcagcttgac catcgtgtta tcacttacgt gatcgctgaa acgcactttg taaccgatgc
    43321 aaccgcccgg ctccgttttc agctcttccg caatacggtt cgccaccgtt cttgccgcca
    43381 ggcgacgcgg ctgggtatgg ccgatcagcc ctttaatccc gcgccccagc tccatacaga
    43441 ttttcggtaa ctgagtcgtt ttaccagaac ccgtttcccc ggcgacgatc accacctggt
    43501 gatcacgaat cgcttcgaga atgtcctgtt ttttctgact aaccggtaaa ttgtcaggat
    43561 aagtaatttc cggtcgtgcc gcttcacgca gcaggacttt ccctgccgcc tggtcaatct
    43621 ctttcgccat ctcctggaaa atggcctgtt gtgcatcagg atttttaacc ttcttcacac
    43681 cgtgcagacg gcgagaaaaa cgcagtctgt cgcgcagcat cagcgaatcc agccgctgct
    43741 gcaaggccgt aaaggtcaat ttttgttgtt ctgtcataac gttagtgggc agtaatctgc
    43801 ctgattaatt ttcttttaaa tgatagatat agagtaccac atcgcggctt tctatgcgtt
    43861 gttcaataaa ttcgaacata ggcttcgatt tattgcgcta tctctgtggc aggattgtga
    43921 ataaagtgtc aacaagcaac ggggcatcgc ccattcaaac atctataagg aaacaccatg
    43981 agcaaggtat tagttcttaa atccagcatc ctggcagggt actctcagtc taatcagttg
    44041 tccgattatt ttgtagaaca atggcgcgaa aagcactccg ctgatgaaat caccgttcgc
    44101 gacctggctg caaatccgat tccggtactg gatggcgaac tggttggcgc tctgcgtccg
    44161 agcgatgcgc cgctgactcc gcgtcagcag gaagctctgg cgctttccga tgagctgatt
    44221 gccgagctga aagcccacga cgttatcgtt attgcggcac cgatgtataa cttcaacatc
    44281 tcaactcagt tgaaaaacta ttttgacctg gttgcacgcg caggcgttac tttccgctat
    44341 accgagaacg gtccggaagg tctggtaacg ggtaaaaaag ccatcgttat taccagccgc
    44401 ggtgggatcc acaaagatgg accaacggac ctggtgacgc cgtatctgtc cacgttcctc
    44461 ggctttatcg gcattaccga tgtgaaattt gtcttcgccg aagggatcgc atacggtccg
    44521 gaaatggcag cgaaagcgca gtctgacgcg aaagcggcca tcgacagcat tgttgctgca
    44581 taaccggaca cgatcttgat gtggactgta gttcagtcca catctcaatc cacttacctg
    44641 ttttcccata atttcagcat cgctttgtga tcttccttaa gcacaatatg cgaacgtctg
    44701 gctcgaatat aactaatcgc ttcatcaacg gttttacagt gtccgtaaca taacaaccat
    44761 gccgccacta ccagcgcact gcgcgataat cccaacgcgc aatggaccag aacgctgcct
    44821 tgctcttcgc gtaatgtttc cagcatcgcc acggcctgtc ggagctcccc ctcttccgga
    44881 accaccagat ccaacatcgg tacacagaaa tagagtcgat cttttgtcgc tcgtccccga
    44941 gggaattcaa aggtgacgtc cagaaccgca ttctgtgccg gaatatgtcg tggaaacgcc
    45001 cctaaataaa caccagcagt aattttgctc accggctcca ggcgacgaca aaaccaacgc
    45061 atagacagcc acatcccgat gcgccagggc aatgtcagcc agtaaacggc gggcgggagt
    45121 ttcccctgac tatctttccc tgttgttatc gcgccaagcc caccgtaccc acggccaata
    45181 atgagtagcg ataatactgg ccaacataac cagactgacc accataactg aatcatcacc
    45241 attagctcca tcaacacaat gcacgagccc gcgcctacga catatggcag tgctattttg
    45301 attcgacgtt gatcaggttt ctgataattc caacgacgat cgacgggcac catccagtca
    45361 atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg ccaggtcgtc
    45421 agagtagaaa tagcgatgag taaaaaccat ccgccacaga ctttacgcca cctctcagcc
    45481 agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag cgaaggagac
    45541 tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcccgt caccccactc
    45601 acttcaggac ggataaaact aaacttcagc ggatagagca aaaaaccgca gcaggccatt
    45661 accgttgcca gaataagccg gtggacaagt cggcgctgtt cgaatgtcgt gctacaaacg
    45721 aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt ccagggaaga
    45781 aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct gttaaggtcc
    45841 tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg ggccagcaac
    45901 aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac gacgcaccgc
    45961 catcgaaacc gtaaaaatac cccactcatc aatccgttgt gtgcatttat caaatccggc
    46021 atcacgcacg agtgaatcca tctccccttg cgaacgcacg cgcatcaccc acggtttacc
    46081 atctttatga ctggttaaca ccccggcaat cagctccagt tgtgggtgcc acggctgccc
    46141 ggtgtagatg agaatgccac ccggttcgat ggcattggca agacctgcga gggagttttt
    46201 tacctgctca ttttcgggaa aaagctcata caggccagag acaatcgcca gcgtaggccg
    46261 cggagttaac gcgctgagtt cctccgggtt aaacgcatcg ccctgttcaa aacgcacctg
    46321 cccagacatt ccccgttggg caatcatctc ttgcccctgt gcaacattta actcactgta
    46381 atcacgtaac aaaatatcgc atacggcagg ttcattctcc agcgcatcca gtacatagcg
    46441 cccatgccct gcggcaatgt caaccacgcg gatggctaaa cctttggcgt ggagatcggc
    46501 aacggcttgt ttaatcagca tttgtaaatg ggttttgcgc tggcgaatac cgcgccagcc
    46561 aacactgttc aggtagattt tgtcgactaa acgcccgaat gcgttactac cttgtggttg
    46621 attttgatag acataatcca gcgaactgcc tgaatcaaag ccggtgctca ttccgagatg
    46681 caggcccgca gagtgcgccc cgaacaattt catcgccata cgcataaagc gatacgccaa
    46741 atcaaccggc gataatggca cgggtccacc agaaagtagc cgccatcgat ccgctgatgg
    46801 tcctgtgcag tcttcatgct gataatcaaa tttttgcgat ttgttagcat ataaatgact
    46861 aataaagctt tgcatttttt caaatgccag cgccctgttc tcttcaccca acgtgtcgtg
    46921 ataaaagcct ggcagcagat gcagctcttt gagagggcta cgtaaacgct gataaaaatc
    46981 gatctgcggt tggcgatgca ccacatagtc atcgcctgat atcagaagtt gcgtggggag
    47041 cgtaatcgcc gccgcatcgc taacaatacg ttcagacgtt ttatagagat cgagcaagat
    47101 attgacggca atcgcccgtg tgatcagcgg atcattattg aaactcgcca cccgttgccg
    47161 atcgtgggtc aaatagcgtc ctttcacata ggaattaata aaaaacagac cacgcagacg
    47221 atgccataac gccagcgcag gacgtgccag cggcacatac aatttaacct taaaggccgg
    47281 agaagccagc accagcccgc gaattgcagg cgcataatca tgaacccatg tggcaaccag
    47341 cactgcgccg acgctttgcg cgatcacaac cacctcttcc agtccaacct ggctgtcgct
    47401 ggcagcaaaa cggacaaatt catcgacatc ctgcactgaa cgcgcaagag atgggctata
    47461 accacgcggc cccgaagttt ttccatgccc ccgggcatcc catgcataaa aagcagtatc
    47521 tggcatcgcc agttcatcaa cgagatgttg tagacgacca gaatgttcat gaccccgatg
    47581 aaataagacg atgacctttt tcgccccggg ctgtaaagcg ggccagtgcc gataaaacaa
    47641 cgctgtatta tcactggtgg taaaaaaatg ttccccaggg atgcgtgaat tttccatttt
    47701 ctctattttc cttcagtaac agctgtagcg aataaaataa aaaaataccg gagcggtgaa
    47761 aattaatgag tcaatcctgt caaggagtcc gccatgtcct ggtaatagtt ttccactatc
    47821 tttaacacca atatctcgtt tgatggctga catcacgaca tcgccgcaaa aaccactaat
    47881 accaattaac aaacccgcta ataatgcctg taatgtattt agcggtgtca gcaacggtcc
    47941 aataatcagt gacgcaatca tggtggtgat gacgcccccc accagacctt ccagcgtttt
    48001 tcccgggctg actttgggga ccacttttct tctgccgcag gattttcccc ataaatactg
    48061 tgcaatatcg tttgactcgg ttaatgccaa aagaaataac accagtaacg ccccgccctg
    48121 gatatttgtg gttggcaaca ttaataacca ggcggcatga ctcaaggcga aaacggtcat
    48181 tatccagccc caaaaaatgg cgctcacggt atgcagaaaa ccggaggggt cccccataaa
    48241 aacttgcccg gtggctaata ttagaaaccc agccaaaggt ataaataata gatacagctc
    48301 aaagcaatta aaaccgataa acaaatagtt aagaggaata acccaatata accaccgcgg
    48361 aaaatgtaca gatataagcg tacaatattc tttcaatgcc agaaaactta tcagcgcaaa
    48421 aaacgtcaac gtcatccagc gtggacctga aatcaccagt gaaaaacaaa tgacgatgcc
    48481 ccaccaggta cgaatacgta atgtaacttc atcctctttt ctctccggta accgccatag
    48541 cagaaaatgg tttatcagcg ttgctaaaat taacaacgcg aaaagtgttg ccagagattt
    48601 ttccagcatt atctttcagc gctcataaga agcacactcc gacaacgatt aatcgcagtc
    48661 cagagaagca gaattgaagc aatgctccat aaaagattat tccactgcat ccactgtgga
    48721 taaatggcaa cggccagacc ccacagacca aatattaacg cgcgatcgct tttgccaaat
    48781 ggaccggcat aactgcgaac accgttaatc gtctgcgcga gtaaaccgca aaactcggtc
    48841 aatatggtgc aaaataacat gagtatcacg agtgatgcgt tactttccgg taaaaataaa
    48901 aatggtaaat agagcgcaat atcggaaata acatcaccag tctcattcaa aattgcccca
    48961 agccgtgttt gctgattgca ctcacgcgcc aacatgccat ccagcgcatt gagcgccata
    49021 cggataaaaa gcacgatggg caatagcata aagaggatgg gttgtgccac caacatcaac
    49081 agcaatccgg taagaagaga aagcgccagt gcagcaagag tgatgtgatt cgctgtaacg
    49141 tggtgcttgt aaagccaaaa catcgtcggc cttaacagcg actgaaagag cggttttatc
    49201 tggtagagcg tcattatcaa tccttgataa ttcggtttgc gctatggtgc atcaattgtg
    49261 atggcggaga tatcacaacg cggagcatca acgtaaattc ggaatatatc aagatgtggt
    49321 ggatggtttt cctacacatc tgtttgatta ccgactggat gtcagtgaga taccatcgat
    49381 cactgtacga atcaatgaat ccattgcctt ttccctgatt atccagccgc aaatcctggc
    49441 cttaaacata tgcactccat gccattaaaa aagaaacaaa agggaaatgc atctttccct
    49501 tttgtatatt cgttattctc gcgaaatacc atccaacgta gacaacactt cctcagaaag
    49561 atgcaatttt tcagcagcca tattctcccg taaatgcgca acagaagacg tccctgggat
    49621 cagcaaaata ttcggtgaac gttgtaacag ccacgccagc gccacctgca tgggtgttgc
    49681 acccaggctc gcagcaacat ccgacagcgt agaggattgc agcggtgtaa agcccccgag
    49741 cgggaagaac ggcacgtagg caatgccgtc acgagccaaa gcatcaatca ttgcgtcatc
    49801 agcacgatga gcaatgttgt attcgttctg cacacagacg atttcggcaa tcttgcgcgc
    49861 ctctgcaacc tgcgtcggtg tgacgttgct taggccaata tgttttacca ggccttgttg
    49921 ttgcatctct gccagcacgg tcaggctggc ttcaatcgat ccttccgctg ggccatgacc
    49981 atcccccatc ataatgcgca agttaaccac atccagtacg tccagcccga gattacgtag
    50041 attatcgtgc accgcttttt gcagctctgc cggagaaaat gcgggcaacc aggatgcgtc
    50101 ctctccacgc cgcgcaccaa ttttagtgac aattgtcagg tcgtcagagt aaggataaag
    50161 cgcttcgcgg ataatctgat tggtgacgtg cggaccataa aagtcgctgg tatcaatgtg
    50221 attgacgccc aatgccagcg cctcgcgcag cacggttata gcgacgtggc gatctcttgg
    50281 gggaccaaaa accccaggac ctgccagttg catcgcgcca taaccaagac ggttaacgga
    50341 ttttgtaccg agagtaaatg tattgctgct catcgcaccc tgccctgtaa taaaacggat
    50401 taagaaaaat cagtatagaa acgaaaagag ataaataatg cgagggcgta aacaagatta
    50461 ctggatgagt tgctctattg tcctgaaaaa cacaaaacaa aacgcgtaaa gcgccgcatc
    50521 cggcaataaa aaaaccgcaa cctgtatcag cgttgcggtt taggtatcac atgctaaaaa
    50581 ttagaatgtt cagtatctgg acgttactgt ttcggtcgca gattctcatc aaataccaga
    50641 cgtttcctgt tcggttcaac ttcccgcaat ggctcaactt gatgcaacgt ctgcttacaa
    50701 cgagtcgcca gcacctgata ctcatgcgta ccctgctttt tccatgccag ctcctgctca
    50761 ctgagttcac gaatcgcttt cgccgggctg ccgacaatca ggtaattggc aggcatttct
    50821 gctttggctt tcacaaatgc ggatgcgcca acaatgctgt tctcgccaat caccgcgccg
    50881 tccatcacta ccgcgttcat tcccactaat gcattgcggc ggataatgca accatgaagg
    50941 atagcgctat gaccaatatg tccatcttct tccacgacag tatcctgctc gggaaaaccg
    51001 tgcataacgc aattatcctg aatgttcgcg ccatctttca ccacgatacg accaaaatcg
    51061 ccacgcaggc tggcatttgg cccaacgtaa acgcccttgc ctagaataac atcgccgatc
    51121 aataccgctg tcggatggac aaaactctct tctggcacaa ccggagtcag accatctatc
    51181 tggtaaattg gcatatcgcc tcctgttcaa tattcaaacc gccaaaacgt tgaaaataca
    51241 ggcttcccgg cgcaggcagt tcaccgaccg aggtttcacc tttttcacta acgaacgcta
    51301 aagcagcagg cgctacgcgc tgataaatgt tgatacacag ctgacgcgcc gtatgccctg
    51361 cccagtgcgc cggaagcaac tcctctggca acaatgggtc tttaaggacg acacggcgat
    51421 aaaaatggat cagtaaaagc tgaatatgaa atgcacgctc cggggttaac tcgtctgctg
    51481 cctcttttaa cagcggcacc agcgggcgga atgactgaat aaaggtttcg tacatggcat
    51541 tttgttcagt taaatgccag cactcttcca ctctggcacg cagtgctgca cgagaaagcg
    51601 ccagtggtat ttgcgcttca aaacaaatca cgttatccac cacccccgct tcatgcaaaa
    51661 gtgtctgtac atccgccagt ttttgcgacg gcgatgccat caggctgggt gccagtgcgc
    51721 caaaaccttg ccagatcaac tgctttttga catcagccag cgttgattta tctaaacctt
    51781 ccgagagcaa caggagccat ttaccatccc atgcaggttg ctctgcgcga taaattttac
    51841 tttctgcccg tcgcgtcagg cgcaagcctt tatcactgag gctatagaaa ctgcgtcgcc
    51901 cgatgcggga aacatccagc cagccttctt tattaagacg aaacaaagcg gtgcgcacga
    51961 aacgctcacc aaatcccagc ccttccagca aagcagccag actacccagc cagatttcac
    52021 caccacgatg ggaaagcgaa tcaccataca gagaggagat caaagatgtg ccactgaccg
    52081 gaacagcgtt tacagcatgt tggataaaag taacaagttt actcattcga ttcagtctgt
    52141 tgttattgtt gtgatttatg aatcatagca caggtcgtat cgacttctgg ccctggcgct
    52201 ttacctcacc agggccagac gacacgtcag gcaccaacaa tattgcgcag atcaaacacc
    52261 cgacacgcct tgccttctga acgcgggata ctgccacagt taacgatcat cacatcggta
    52321 gagatcccca ccatcgactt aatccgatgg cgcagctggt ggcacacctg gcaacgctgc
    52381 tcatgcgtca atgtcaggct gctttctttc aactccactt tcacagaaag tgaatcaaga
    52441 tgcccccggc ggttcacctc cagttggtaa tggggcgata aatgttcgaa tttgacaatc
    52501 tcttcttcca gttgtgacgg gaagacattt acaccgcgaa tgatcaacat gtcatcgctg
    52561 cgtccgctga tgcgatccat gcggcgcata gtccgcgccg ttcctggtaa cagtcgggtt
    52621 aaatcacgcg tgcggtaacg aatgaccggc aatgcttctt tggtcagcgt ggtgaataac
    52681 agttcgccat gttcaccatc ggcaagcggt gtgccgtcat gaggattaac aatttcagga
    52741 tagaaatgat cttcccaaat ggttgggccg tcggtagttt ccagacactc catcgccacc
    52801 cccggcccca tcacttctga caggccataa atatccagtg cggtgatccc cagacgccgt
    52861 tcaatctctt tacgcatggc ctgtgtccac ggctcagcac caaacactcc aacccgcagc
    52921 gagcaaccgc tggcatcacc gcccaactgc cgctccagct cttcaatcag gttaaggcaa
    52981 taagatggcg taaccatgat catatctggt tgaaaatcgc ggatcagttg cgcttgtttt
    53041 tccgtctgcc caccagacat cgggatcacc gtagcgccca gacgttcggc accatagtgc
    53101 gcgcccagcc caccagtaaa caggccgtaa ccataggcaa catgaatttt gtctttgggc
    53161 gtgccacctg ccgcacgcaa ggaacgcgct acaatattgg cccagttatc aatatcgttt
    53221 tgcgtatagc cgacaactgt cggtttacct gtggttcccg aagaagcatg aatgcgcacc
    53281 acttgttcca tcggcacggc aaaggtgtcg aagggatagt tgtcgcgcag atcctgtttg
    53341 gtggtacagg gaaatttacg cagatctgaa agttccctga aatcatcagg atgtacgcct
    53401 gctgcgtcaa atttgcgccg atacatcggg acattttcat acgcgtgttt gagcgtccat
    53461 ttcagacgtt gtgtttgcaa cgcctgtaac tcatcaacag acgcggtttc aatcgggtca
    53521 agctttgtat tggttatcat tgtagggtac tcgcaggttg atatgctcaa acacgctcca
    53581 gaatcatggc gatgccctga ccgacaccga tgcacatggt gcacaatgcg taacgaccgt
    53641 tacgccgatg cagctcatgg ctggcagcca gtgccaggcg ggcaccactc attcccaacg
    53701 gatggcctaa ggcgatagcg cctccgttgg ggttaacatg tggggcatca tcaggcagcc
    53761 ccaattcgcg tagtacaccc aacgcctggg ccgcgaacgc ttcgttcagt tcaatcacgt
    53821 ccatatcgtg aatactcagc cccgcgcgtt ccagcacccg gcgagttgca ggcaccggac
    53881 caagccccat caggcgcggt tccaccccgg cggttgccat ggctacgatt cgcgcccgcg
    53941 gtgtcagtcc ttgcgctgct gccatctgtt cactggcaat aatcaacgca gcggctccgt
    54001 cattcacccc ggaggcattg cctgcggtaa tcaccccatt ggcacgaaat ggtgctttta
    54061 acccacgtaa ctgttccagc gtcgtttccg ggcgcagatg ctcatcatgt tgtatttctg
    54121 ttacaacgcc tttcttgttt ttcaacacaa ccggaacaat ctcctcagcc agaatgcctg
    54181 aggattgcgc ttttgccgta cgttgctgac tgcgtagcgc aaaactatct tgatcttctc
    54241 gcgatatttt taacagttca gctacattct ctgccgtttc cggcatgctg tcagttccaa
    54301 attgttgagc catgagcggg ttcacaaatc gccagccaat agtggtatca aacatctcag
    54361 cctgacgaga aaatgcactg gttgccttgc ccataacaaa cggtgcccga gacattgact
    54421 ccacgccacc agcgatcagc aaatcgccat cgcccgcttt aatcgcccgt gcggcgaacc
    54481 ccagtgcgtc cagcccggaa ccacacaagc ggttaatggt tgtgccggaa acactctgcg
    54541 gcagccccgc cagtaacgtc gccatccggg ctacgttacg gttgtcttct cccgcctgat
    54601 tagcacagcc gaggatcaca tcatcgatac actccgcatc gagacgcggg tttcgcacca
    54661 gcagctcccg caaagggata gcagccagat cgtcagcccg aacacctgat aatgccccac
    54721 catagcggcc aattggcgta cgaattccgt cacagataaa ggcttcacgc atcaggcttc
    54781 tcctgtaatg gtgccgccga tgcggtgaga tttaccgcga aacagcgcaa ccgttttttg
    54841 ttgttggtta acaatttcga tgtcgtaaac accggtttgc ttgccctgat gacgtacctg
    54901 cgcagtagca gttaaggtgt ctccggcaaa gcctggacgt aaaaaatcaa tcgtgcaggc
    54961 agaagcgacg gctgccagcc cctggctatt gcaggcgtag gcaaaggcag tatcagccag
    55021 tgaaaatagc tgcccgccgt ggcaactttg atgaccgtta agcatttgtg cagtgacggt
    55081 catggtcact acagcaaagc cttcatccat tgagataatg tcgatgccaa gcgctttggc
    55141 gcaggcatcg ttctcataca ttgcatgggc attttgccag gccttatgac tcataaccgc
    55201 tctccagaag cgcccgttgg cgcagcaatg aacatgggcg atagcgttct tcgccatagt
    55261 gatgctgtag attttcaagg agccttaata ttcgctgcca gccaagttgc gctccccagg
    55321 caagtgggcc atatggataa ttcaccccaa gacgcatggc ggtatcgata tcctgttcag
    55381 aggccacgcc tttttgaagc gcatcaaggg cttcattgat gatcattgct accgttcgcc
    55441 aaatcagcat tcctgggtaa tctgcaattt gcagcactgt tttgccctgc tgttgcaggt
    55501 aataaatggc cttgcgggtc gctgagtccg ggttcaccgc tgccgcagca atggtcacca
    55561 ccttgcccgc cattttgtcg accactacca ccgggcgtgc cagtcgtatt gccagcgcct
    55621 gtgccgtctc gccttgtgtc tcaatcaata aaacatcgtc aatttccgtg acaccgtcac
    55681 ttttcttttc tactttcatt gggctaaaac tgtcgcttac cgcttccagg ccaacaactg
    55741 cctcacgttc cgcgcgccag tcgtacacgc ccagcccact tttcttgccc aaccgtccac
    55801 caatcaccag ttcctgttgc accagcgaag gtaaaaaacg acgctcctgc cagaaagcgt
    55861 taaacaccga acaggtgaca gcaaaattga cgtcctgacc aatcagatcg gttaattcca
    55921 gcggccccat cgggaaacca gcgccatcgc gaagtgcagc gtcaatcact tctggtgcag
    55981 caacctgctc ttccagtgcc cgccaggcct cggaataata aggacgcgca acacggttaa
    56041 cgataaatcc gggggtcgaa tgacagcgca ctggctgctt accccaactc aatgtcagtt
    56101 cacacaactg ctcgacaact tccgctgccg ttgccagccc actgaccacc tccaccaact
    56161 tcatcaccgg tgccgggtta aaaaaatgca gccccgcaac acgttcagga ttttttacct
    56221 ccgcagcaat cgcggttata gagattgacg aagtgttagt ggtcaatagc gtttgtggcg
    56281 ggcaaacttc cgccagttgt gcaaagagcg cttttttaac ttccagacgt tcagacgccg
    56341 cttcaataac cagattcgct gctgccagcg cgtgaatatc ggtcaccggg atcaggcgtt
    56401 tcaatgtgcg ttcacaggtt tcagcggtca gttttccccg cgtcacgcgt gaatttagcc
    56461 gcgcgtgtat cccgtcgatt gcgcgggtca gcgcttcagc agaaatgtca tacagtaaaa
    56521 cctgatgtcc atgactggcg gcaacttcag caatgcctgc ccccatggtg ccgctcccaa
    56581 tcactgccac agtttgcaca tttatcatca tgctatttcc ccgtgaactg cggtgagcgt
    56641 ttagccagga acgcactgac accttcacga taatctgcgc tccgtccggc aagtcgctga
    56701 tagtcacgtt ccagatccag ttgcgtatcg agcgtattgg tttcagcgct atttatcgct
    56761 tgcttgataa gtccaagacc aaatgtcggt tgtgtcgcca gatgccgtgc cagctgttgc
    56821 gcggtatctg ccagcgtttc atcatcaaca acctgccaga tcatccccca ttcgtgcgcc
    56881 tgttcagcac tcagttgatt ccccagcagt gccagcccca tggcgcgcgc tcgtccggca
    56941 acgcgtggca gtaaccaggt tccaccgcaa tcgggtatta agcctaactt actaaacgcc
    57001 atgacgaatt ttgctgaacg ggcagcaata acgatgtcgc accccagtgc cagtgttgcg
    57061 cctgcccctg ccgccacgcc attgactgca cagatcaccg gttttggcag ttttgccagg
    57121 cgacgtacca gtgggttata gaaacgttca actgacatcc ctaaatccgg tgcggggcct
    57181 gtgggatcaa cgttacgatc gttaagatcc tgaccagcac aaaatccgcg cccggcacct
    57241 gtaagtaaca ggcaacggat agtgtcgtcg cgctcgacct gtttcaggca ctctgccagt
    57301 tgtgcgtgca tctcatcatt aaaactgttc aggcgttccg ggcggttgag cgttagtgtc
    57361 atcacgccct tttctacatg actgaggatg aattccatca ttagcgtcct ttaaagtcgg
    57421 gcgtgcgttt ttgtaagaaa gcggagatgc cttcatgacg atcttctgtt gccgccagca
    57481 aggtgaataa ctgtcgctcc tgggcaagtc cggcttgcaa agccacttcc tgcgactggc
    57541 gcagcgcttg ctttgccgct tgtaaggcca gcggcgagtg acgtgccatt ttcgatgcca
    57601 gctgtaaggc gtattcgagg gttaaatcgc tggggaagac gtcgctaacc agcccggcct
    57661 gctgtgcttg ctgggcggtg atactttctc cgctcagcac cattttgctg gctaacgatt
    57721 taccgacact acggattaaa cgttgcgttc cgcctgcgcc aggcatgatg ccgagagtga
    57781 tttccggcaa cccaaaacgc gcgttctctc cggcaaccac cacatcgcac aacaatgcca
    57841 gttcgcaacc cgccccaagc gcgtaaccat tgacggctgc gataagtggt ttattaaagg
    57901 cctgcaatcg cgcccatagc tgcggacgtg tatcgtttaa ggtggccgcg agatcttttt
    57961 ctgccatttc gttgagatcg gccccagcgg caaaaaagcg tgcattaccg gtaatcacac
    58021 agaccgaaat gctggtatcg gtagccgcag cttccagttc atttaccagt tgcatcagca
    58081 gggcattatt tagcgcatta cgtgcggcgg gacggttaag ggtcagcaac aatactcgtt
    58141 gctgacggct gacgatcagt tcgctcatgc catccccttc gcgtcaaagt caaccaccac
    58201 atcactggtc agcggcagtg cctggcaact caacacatag cctgcagcca gttcatccgg
    58261 ttccagactg taattggttt ccatcgccac tttgccacgc agcactttgc atttgcaggt
    58321 cgcacagacg ccgcctttgc aggcataggg cagatcagcc ccctggcgca atgccgcatc
    58381 gagaatgctt tcatcgtcgg cattaagcac gatttcccga tcccgcccat cctgacgtac
    58441 agtcactttt tgtccgtcac tttgcacgtt aacgctacgt ttgacgcgcg tgccaggcgt
    58501 attaaaccgc tccagatgaa tggttttatc tggcattccc agtgctttta aggcggcttc
    58561 cgtctcatcc atcatcgccg ccggaccaca aataaatgcc tcatcataaa gacgaaaatt
    58621 aatgagcgag gccccaagtg actgtaattt ttcaccgtca atacgcccgt gaagcagatc
    58681 gctgtcgagg gtttcctgac tgaaaatgca caacaactgt aaacgctgag gatatttgtc
    58741 tttcaggtct gccagtgcct ggcgaaacat catgctctgg ctggtacggt taccgtagat
    58801 cagggtgaac tgactttcag gctcggtttg taaagtggcg gcgataatcg ccagcattgg
    58861 cgtaatacct gatcctgctg caatagccag atagcgcccc tggcgttcgg cctgcggctg
    58921 atagccgaaa tggccctgcg gcaccatgac ttccagcgtc atcccctgac ggatgtgatc
    58981 gcgggcatag cgggagaaac gtccgccttc aatggctttt accgccacac taatttcgcc
    59041 aggcaggtag ctgcggcaaa tggagtaaca acggcgtaat tcttcaccat caaggctggc
    59101 ttttaaggtc aagtgttgac cggggcgaaa gcgatacgcc tcctgcaaag gctggggcac
    59161 cgcaaaggta atggtcaccg catcacgggt ttccggctcc acttttgcca ccgttaagga
    59221 atgaaacgtt gtcatggcat cctcaaatac atttgaaata atcgaaaggt tcgcggcaac
    59281 tatcgcagcg atacaatgct ttgcaggccg tggaaccaaa ttcactgata agtgtggtat
    59341 ggacgctggc acagcgcggg caacatactt ctggcggcaa atgggcatgg caactgtgtc
    59401 cggcgggcgg gctaatgcca tactgtcgca gacgctcacg ggcatcgggg gtcatccagt
    59461 cggtggtcca tgccgggtca agttgcagca caacctgaac gggggtaaac ccgtgggttg
    59521 acattgcctc acgtatcgcg ccaatcaaat gttccgttgc cggacaaccg gaatatgtcg
    59581 gcgtaaagcc gatcacccat ccttctccca tctgtgtcac attacgcacc atgcctaaat
    59641 cagtaatggt cagcaccggg atctccgggt ccgggatctg gctgagtagc gcccatatct
    59701 catgaacctg tggcggtgca atggtggcca gacgttgcat acccatctcc tctgttacca
    59761 ttgctgaccg ggcaagacac gctggagata ctgcatttct gccagcatgg gtccaaggtg
    59821 ttcggtatgc agtccttttt taccgccagt gcgatacgcc tgctcttgcg gtacgttcaa
    59881 tgtggcttcg ttgatcccag caaaaacttc ggcttcccac gctgcgcgta aagtgcgtgg
    59941 atcaaccgca ataccctctt cactcagtgc aatatcaatc tcgtcggcat cgaacagttc
    60001 ggcggtaaaa cgccacaact tgttgatggc ctgctgcatc ttttgccctg atacgtcagt
    60061 accattgccc agtcgctcca gccagccacg actaaaacgc aggtgataac gcgcttcttt
    60121 aattgctttg gcagaaatcg ccgccagttg cggatcacgg ctctccatca gacgggtaaa
    60181 gagcgccaca tgccatgcat cgatgaaata ctggcgtgca atggtgtcgg caaaattgcc
    60241 gtttggctgt tcaaccaaca ataagttgct gaactggcgc tcgtctcggg tgaaggccag
    60301 ggtatcttca tcgccttctc ccgctaattc agcggcatac gataagaagt tgcgtgcctg
    60361 gcctaataaa tcgagtccaa tatttgccag tgcgagatcg atttccagtt ccggtgcgtg
    60421 accgcaccat tcccccaacc gctgggagag caccagacag ttatcgccca ggcgcaaggt
    60481 gtaagccgtt aactgattca tttccgacct cacatgtgct caatgccatc agggatggtg
    60541 taaaacgttg gatggcgata gaccttgctt tcagccggat caaaaaattc accgcgttct
    60601 tccggttgcg aggcaacaat ttcactcgcc ttcaccaccc aaattgaaca tccttcgcta
    60661 cgacgggtgt aagcatcacg ggcattttcc agtgccatcc gctcatcggc agcatgtaaa
    60721 ctgccaacat ggcggtgtga taagccctgt ttgccacgca cgaaaacttc gtataacggc
    60781 cagtaaacat tactcatttt gaatctcctt atgcgacctt acgggcaagt tgtttttgtg
    60841 catgggccag cgctgcttcc cgtacccagg taccttcttc ccaggcttta cgtttggcgt
    60901 cgagcctctc ctgattacaa attccgcgac cgttaattac ttcgttaaac tcctgccagt
    60961 cgatctcacc aaagcggtag tgaccgcttt cagtgtcaaa atgcagatcc gggtcaggaa
    61021 cggtcatacc gagcatttca acctgtggaa cggtgttgtc cacgaaacgc tggcggagtt
    61081 cgtcgttggt gaaacgtttg attttccagg cgagacttct ggcgctgttt ggcgagttat
    61141 catcgtttgg cccgaacatc attaaggctg gccaccagaa acggttaatg gcgtcttgca
    61201 acatctgctt ttgagcttcg ctgccctgcg ccagtgccat gcaggcttca aacccctgac
    61261 gctggtgaaa actctcttct ttgcagattt tcaccattgc tctggcatac gggccataag
    61321 aagtgcggca taacgccacc tgattgacga tcgctgcgcc atccaccagc cagccgataa
    61381 caccgatatc agcccaactc aatgtcggat aattaaagat ggaggaatat ttcatccgtc
    61441 cgtcgagcat tttttggtag atgtcttctc tggcgcagcc cagtgtttcg gcggcgctat
    61501 agagatacag accatgaccg gcttcatcct gcactttggc caacagaatg gctttacgcc
    61561 gcaaggttgg tgcgcgagtg atccagttac cttcaggcaa cataccaaca atctcggagt
    61621 gcgcatgctg cccaatctgg cggatcagtg tctttcggta agcatcgggc atccagtcct
    61681 gcggctcgat agccgtttcc tgggctatcc gttgctcaaa gcgttcttct tgggtcacgt
    61741 catcaccttt acgattccag ttttcgtgaa ttaattaaaa acacgaatca cgttgtttta
    61801 tgaaagttac acaatggtta actaaaatca caaattttgt atgtttgaac tgtgacggat
    61861 ttcgcaccct atttttataa ctcattatat atcagtgcat taatgttttt agtggttgat
    61921 atacagatcg catagttaac atttcgttaa aatacgcctt gctttttatg attcgtgatt
    61981 taactattag caacagaaat gtgaaacatc tggagagtag cgatgcagca gttagccagt
    62041 ttcttatccg gtacctggca gtctggccgg ggccgtagcc gtttgattca tcacgctatt
    62101 agcggcgagg cgttatggga agtgaccagt gaaggtcttg atatggcggc tgcccgcctg
    62161 tttgccattg aaaaaggtgc cccagccctt cgcgctatga cctttatcga acgtgcggcg
    62221 atgcttaaag cggtcgctaa acatctgctg agtgaaaaag agcgcttcta tgctctttct
    62281 gcgcaaacag gcgcaacgcg ggcagacagt tgggttgata ttgaaggtgg cattgggacg
    62341 ttatttactt acgccagcct cggtagccgg gagctgcctg acgatacgct gtggccggaa
    62401 gatgaattga tccccttatc gaaagaaggt ggatttgccg cgcgccatgt actgacctca
    62461 aagtcaggcg tggcagtgca tattaacgcc tttaacttcc cctgctgggg aatgctggaa
    62521 aagctggcac caacgtggct gggcggaatg ccagccatca tcaaaccagc taccgcgacg
    62581 gcccaactga ctcaggcgat ggtgaaatca attgtcgata gtggtcttgt tcccgaaggc
    62641 gcaattagtc tgatctgcgg tagtgctggc gacttgttgg atcatctgga cagccaggat
    62701 gtggtgactt tcacggggtc agcggcgacc ggacagatgc tgcgagttca gccaaatatc
    62761 gtcgccaaat ctatcccctt cactatggaa gctgattccc tgaactgctg cgtactgggc
    62821 gaagatgtca ccccggatca accggagttt gcgctgttta ttcgtgaagt tgtgcgtgag
    62881 atgaccacaa aagccgggca aaaatgtacg gcaatccggc ggattattgt gccgcaggca
    62941 ttggttaatg ctgtcagtga tgctctggtt gcgcgattac agaaagtcgt ggtcggtgat
    63001 cctgctcagg aaggcgtgaa aatgggcgca ctggtaaatg ctgagcagcg tgccgatgtg
    63061 caggaaaaag tgaacatatt gctggctgca ggatgcgaga ttcgcctcgg tggtcaggcg
    63121 gatttatctg ctgcgggtgc cttcttcccg ccaaccttat tgtactgtcc gcagccggat
    63181 gaagcaccgg cggtacatgc aacagaagcc tttggccctg tcgcaacgct gatgccagca
    63241 caaaaccagc gacatgctct gcaactggct tgtgcaggcg gcggtagcct tgcgggaaca
    63301 ctggtgacgg ctgatccgca aattgcgcgt cagtttattg cagacgcggc acgtacgcat
    63361 gggcgaattc agatcctcaa tgaagagtcg gcaaaagaat ccaccgggca tggctcccca
    63421 ctgccacaac tggtacatgg tgggcctggt cgcgcaggag gcggtgaaga attaggtggt
    63481 ttacgagcgg tgaaacatta catgcagcga accgctgttc agggtagtcc gacgatgctt
    63541 gccgctatca gtaaacagtg ggtgcgcggt gcgaaagtcg aagaagatcg tattcatccg
    63601 ttccgcaaat attttgagga gctgcaacca ggcgacagcc tgttgactcc ccgccgcaca
    63661 atgacagagg ccgatattgt taactttgct tgcctcagcg gcgatcattt ctatgcacat
    63721 atggataaaa ttgctgctgc cgaatctatt tttggtgagc gtgtggtaca tggatatttt
    63781 gtgctttctg cggctgcggg tctgtttgtc gatgccggtg tcggtccggt catagctaac
    63841 tacgggctgg aaaacttgcg ttttatcgaa cccgtaaaac caggcgatac catccaggtg
    63901 cgtctcacct gtaagcgcaa gacgctgaaa aaacagcgta gcgcagaaga aaaaccaaca
    63961 ggtgtggtgg aatgggcggt agaggtattc aatcagcatc aaaccccggt ggcgctgtat
    64021 tcaattctga cgctggtggc caggcagcac ggtgattttg tcgattaatc ggtgaatgaa
    64081 gggcaacggc gaatagttgc ccttttattt cactaaattt tgtgacgttg tcacattatg
    64141 catgatgtgt acatctattt tcagggcatc cactgtatga aaagctggca cacctgccaa
    64201 acaacctggc aggtgcaggc aatccccttt gcatcagtac tgataatgtg aacctgacta
    64261 aaccgcccac agagcgcggt tgctaacaag aacacaacat ctgacgaggt taataatggg
    64321 aagcccctct ctgtattctg cccgtaaaac aaccctggcg ttggcagtcg ccttaagttt
    64381 cgcctggcaa gcgccggtat ttgcccacgg tggtgaagcg catatggtgc caatggataa
    64441 aacgcttaaa gaatttggtg ccgatgtgca gtgggacgac tacgcccagc tctttaccct
    64501 gattaaagat ggcgcgtacg tgaaagtgaa gcctggtgcg caaacagcaa ttgttaatgg
    64561 tcagcctctg gcactgcaag taccggtagt gatgaaagac aataaagcct gggtttctga
    64621 cacctttatt aacgatgttt tccagtccgg gctggatcaa acctttcagg tagaaaagcg
    64681 ccctcaccca cttaatgcgc taactgcgga cgaaattaaa caggccgttg aaattgttaa
    64741 agcttccgcg gacttcaaac ccaatacccg ttttactgag atctccctgc taccgccaga
    64801 taaagaagct gtctgggcgt ttgcgctgga aaacaaaccg gttgaccagc cgcgcaaagc
    64861 cgacgtcatt atgctcgacg gcaaacatat catcgaagcg gtggtggatc tgcaaaacaa
    64921 caaactgctc tcctggcaac ccattaaaga cgcccacggt atggtgttgc tggatgattt
    64981 cgccagtgtg cagaacatta ttaacaacag tgaagaattt gccgctgccg tgaagaaacg
    65041 cggtattact gatgcgaaaa aagtgattac cacgccgctg accgtaggtt atttcgatgg
    65101 taaagatggc ctgaaacaag atgcccggtt gctcaaagtc atcagctatc ttgatgtcgg
    65161 tgatggcaac tactgggcac atcccatcga aaacctggtg gcggtcgttg atttagaaca
    65221 gaaaaaaatc gttaagattg aagaaggtcc ggtagttccg gtgccaatga ccgcacgccc
    65281 atttgatagc cgtgaccgcg ttgctccggc agttaagcct atgcaaatca ttgaggctga
    65341 aggtaaaaat tacaccatta ctggcgatat gattcactgg cggaactggg attttcacct
    65401 cagcatgaac tctcgcgtcg ggccgatgat ctccaccgtg acttataacg acaatggcac
    65461 caaacgcaaa gtcatgtacg aaggttctct cggcggcatg attgtgcctt acggtgatcc
    65521 tgatattggc tggtacttta aagcgtatct ggactctggt gactacggta tgggcacgct
    65581 aacctcacca attgctcgtg gtaaagatgc cccgtctaac acagtgctcc ttaatgaaac
    65641 catcgccgac tacactggcg tgccgatgga gatccctcgc gctatcgcgg tatttgaacg
    65701 ttatgccggg ccggagtata agcatcagga aatgggccag cccaacgtca gtaccgaacg
    65761 ccgggagtta gtggtgcgct ggatcagtac agtgggtaac tatgactaca tttttgactg
    65821 gatcttccat gaaaacggca ctattggcat tgatgccggt gctacgggca tcgaagcggt
    65881 gaaaggtgtt aaagcgaaaa ccatgcacga tgagacggcg aaagatgaca cgcgctacgg
    65941 cacgcttatc gatcacaata tcgtgggtac tacacaccaa catatttata atttccgcct
    66001 cgatctggat gtagatggcg agaataacag cctggtggcg atggacccag tggtaaaacc
    66061 gaatactgcc ggtggcccac gcaccagtac catgcaagtt aatcagtaca acatcggcaa
    66121 tgaacaggat gccgcgcaga agtttgatcc gggcacgatt cgtctgttga gtaacccgaa
    66181 caaagagaac cgcatgggca atccagtttc ctatcaaatt attccttatg caggtggtac
    66241 tcacccggta gcaaaaggtg cccagttcgc gccggacgag tggatctatc atcgtttaag
    66301 ctttatggac aagcagcttt gggtaacgcg ttatcatcct ggcgagcgtt tcccggaagg
    66361 caaatatccg aaccgttcta ctcatgacac cggtcttgga caatacagta aagataacga
    66421 gtcgctggac aacaccgacg ccgttgtctg gatgaccacc ggcaccacac atgtggcccg
    66481 cgccgaagag tggccgatta tgccgaccga atgggtacat actctgctta aaccatggaa
    66541 cttctttgac gaaacgccaa cgctaggggc gctgaagaaa gataagtgat tgtttcagac
    66601 aaaaaaacgg caccaggtgc cgttttttac ttatgagcga accagattaa taccgtacac
    66661 acaccgactt agtttcacac caaccgtcca gccagtcggg gccaaaatca cggcccgttc
    66721 ctgactgctt catcccacca aacggtaagt tagcgtcaat taaggtatgg ctgtttaccc
    66781 acaccgtccc tgcctgtaag cgatcgctat attccagagc ctgggagaga ttttgcgtcc
    66841 agacactggc agttaagcca tattccgtgt cgtttgccag ttgtaacgcc tcttctccat
    66901 ccgctactcg caccaggttt accaccggac caaacacctc ttcacgagtt aagcgcaatt
    66961 tagcatcggg atttaccacc agcgttggcg caacataata cccctctccg gctggtccat
    67021 tcgacccgcg aatcagctct gcttgctgtg cctgcgcatc gtcgaggaat gaacacactt
    67081 tgtcgcagtg cgcacgagaa accaaagggt taatctgtgc aacaggtgac atccccggtc
    67141 ccacttgcaa cgattttacc gcctgctcaa atccactaac cagcgtgtca aacaacggcg
    67201 cttcaatata aattcgcgaa ctggcggcgc atacttgccc ttgattcagg aagcttccgg
    67261 tcatcaagcc ttcaataacc cattgcggat cagcatcttt taatacaatt gccgggtttt
    67321 taccgcccag ttccagcgtt acacgcgtta agtgatcagc agcagttctg gcaatacctt
    67381 ttcccgtcgc ggttgaaccg gtaaaactga ttttcgcaac atgaggatgt gacgtcaggg
    67441 ccgcgccgca tacagcacct gacccggtga cgacattaaa aacgccatca gggataccag
    67501 cctcgctggc cagttccgcc acgcgcaaca tcgtcagtgg cgtggtttcc gaaggcttaa
    67561 tcacgattga acagcctgct gccagtgctg gcatcacctt ccacatacca atcatcaacg
    67621 gaaagttcca tggcacaatt cccgccacta cgccaaccgg ctctttacgc gtccaggcct
    67681 gataacgcgc cccctggggt aagggaatcg acaagtccag cgttttaccc gcgattttgg
    67741 tcgttaaccc ggcggtataa cgcatccagt tcagcgtaca gcccacttca aaagcacggg
    67801 aaatggcaat tgacttgcct tgctccaggg tttccagttg cgccagctcc tcactgtgct
    67861 gttccaccag atcggcaaaa cgtagcagaa tacgttcacg ctctgcgggt aatcgcccgg
    67921 cccagcgacg cgagacaaag gcccgccagg cagacatgac tgcgttatct acatccgctt
    67981 cgttggcatc agcagtagac gcaatttctt gcccggtggc cggatcaaag atcgccaacc
    68041 gtttttcact ttgtgcgggg ccaggacgac catcaatata aagaccgtgt tgacgatcga
    68101 gaaactgttg gacctggctt aatactgcta catgcggctc tgtcatcact tttccttatt
    68161 atttacccag tgtgatttca gtgtacgcag cgacaagaaa agtcactttt ttcgctgtga
    68221 cattcgcttt gtcagctgtg acaagctccg caaatcgtga caataaccgc ttatgtctct
    68281 atgcctgaca agacttattt cctatagtca ggctcattag cttcgtttat tgcaacacaa
    68341 atgcaacaat aaaaatacat ttcacagagc gaaaacgtgc catgaacccc gcaatggata
    68401 atgagtttca gcaatggctt tcccaaatca atcaggtatg cggaaatttt accggacgcc
    68461 tgctgactga gcgttacacg ggtgtactgg acacgcattt tgccaaagga ctaaagctga
    68521 gtaccgtgac aaccagcggg gtgaatttat cccgcacctg gcaggaggta aaaggcagcg
    68581 acgatgcctg gttttacacc gtttttcagc ttagtggtca ggcaataatg gagcaggatg
    68641 agcgtcaggt gcagattggc gctggcgata ttacgttact cgatgcctca cgcccctgtt
    68701 cgctttactg gcaggagtct tctaaacaga tttcattact tttgccacgc actctgctgg
    68761 aacaatattt tccccatcaa aaacctgtct gcgcagaaag actggacgct gacttaccca
    68821 tggtgcaact cagtcatcgc ctgttacagg agagcatgaa taatccggca ctttctgaaa
    68881 cagaaagtga agctgcgcta caggcgatgg tgtgtctgct gcgcccggta cttcatcagc
    68941 gggaatctgt tcaacctcgt cgtgaacgtc agtttcaaaa agtggttacg ttgatagacg
    69001 ataatattcg cgaagagata ttacgcccgg agtggatagc cggagagaca ggtatgtcag
    69061 tacgtagttt gtaccgaatg tttgccgata aaggtttggt ggtcgcgcaa tatattcgta
    69121 accgtcgtct cgatttttgt gcagatgcga ttcgccatgc cgcagatgat gaaaaactgg
    69181 caggcatcgg ctttcattgg ggattttctg accagagtca tttttcaacg gtatttaagc
    69241 aacgctttgg gatgacgcca ggcgagtatc gacgtaaatt ccgctaacta attggcgttg
    69301 tgtgtactgc aacgccaatt agttaaaaca gatatttgac tatgcatttt tcagggcgaa
    69361 aggcaatttg acaggagttt ttgaatattc accttgtttc aggaaaatag atcaatcgct
    69421 ttctcctgta gcgttatccc gatattgcgg ttttgctatt aaaattttcg cacccattta
    69481 ccgtttatcc cttgtacata ttcccccggt ttggcccgag ctacgagctt ttgcccggcg
    69541 agtttcgcga tatcatctac agacacatta ttttgctttg ccagttgttg gtaactcgct
    69601 ttacgttcgg cattaatgtc ggttactaat ttctctgttt cagcatccgt tttcagcgca
    69661 accagataac cataaaatgt ttcacctacc cgcccctgag ttctggcttc atccagagtc
    69721 aatgccatta cattgctgct taccagccca acaagaaacg cacaaagaag taatgttttc
    69781 ttcatcatcg cctcagaaaa gatcgctacg agtttcaagc agctcttcga catctttgtc
    69841 tgccttgatg atgatctcat gctcaatttt aacgttcata ttgatagtga tcggttcctt
    69901 gggcgcagcg acttcaatac gcggcgtaca ggcagccagc ataaaagatg acgtcaacgc
    69961 agccagtaaa attttcattg tttttcctca cactcttttc cttgcggaca gtcatttccc
    70021 ggcagacgtg cgttctgctc caaccatgcc tggagattat cgccaaagcg taaactgcgc
    70081 cacagcgtaa aaatattctc ttcatgatgg taattaaggt ttaccgtacc gcttttacca
    70141 tcaacgcgac tggtacctgt aatgttggcc tgcatggtta ataaaccgag attatctaaa
    70201 ttaatatttg tcgatgaacg gctaatttcc atatagcgca accagttaat tgctgaaccc
    70261 gcagtcatat tgtctttcac caccgcatct gcggtgtctt tatccagccg caaggtcatt
    70321 ggcccgctat ttgccagcca accatctttc actatccatt tttcgttgtt tagccataac
    70381 ggcaatgcac cactaaatgc cccggacatg gcgaattgtt tcggattgac ggcgctaacc
    70441 agttcgctgg atgagagatt attcagccgc aacagcgccg ggtcatgttg cggcatacgt
    70501 aattgtttca tcagtacgtt accgcctaac acatcgacgc taacatcagt caacagcaag
    70561 ggttcttctt cagtccacgg ataacgccct tgcaaatcag ccgtaatatt tttcgctgtc
    70621 accagattaa tcacttcggc aattcgcaac gtaacggggc cgcgagtccc cagatgccag
    70681 gctccatcgg caaaacggaa aggcaggaca aaatcgacac cgttaacctg attatctggc
    70741 atccaggcac taccgccttt caacacgccg tgtcctcccg cgcggaatcc ttgttcaggc
    70801 gcagcagaaa atgcaacctg agcatacagt tcaccatcgc gtaagttcat cttccagtcg
    70861 ggtggcacca gcggctggaa tacagtcagt gattgttttg gccaccaggc gttaccgcgc
    70921 agacgaatac cgtcccagcg accatttacc cgaaccgggc caatctcacc agcatgtaaa
    70981 tcgcctttaa agaggaaata cgtcggatcg cgcccatcga cgctaaattt taaggttgat
    71041 ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg cgtccagtga cagcgcgccg
    71101 ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg gcttgtcgag aattaatcgc
    71161 ggcttttcta ccgtcatcgt accgtattgt aactggtcga aaccggtgga gaggtcggtc
    71221 agcgtaatcg tgctgtcatg ccattcacct tttcctgcga catcccaggt ggcattcatc
    71281 ggtgtaaaac tgccttttcc ccagtagcgc cactgccagc ggcccgcgtc agggagaaaa
    71341 tcattcgcca gcccatccat atgcagcacg aaatcgccca gttcattttc atgcgcctgc
    71401 aagatggcct gcaaacgtcc gtcaacacca cgttgggtga cttttacacc cgctaaaggc
    71461 cagcggattt catcgatgtc cagcgaatcg atgactcttc ccttcgaacg taataacgcg
    71521 cctggctcaa aggccaacgt tgggtcagtc agacttccgc ttagctgcgc aggtaaacgg
    71581 gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca gttgcagagg cagctgactg
    71641 ttatccatgc ttaattttcc tgggccaaaa ttaagcaccg cgttgccttt acccgcttgc
    71701 ccctgggtca gcacactcag tcgtccgctg accagagcgt tctcgagccc tgcctgccag
    71761 ttgtcgactt tgacacccag tcggccactc aaaggaaaac ctgcatacgg ccagctccag
    71821 cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc acggcaaatc gagcaacgga
    71881 tcgccgttat cccgtgccag cacgatcaat tgcccgctat tttcctgcca gtccagctcg
    71941 gcatccacca gtgaaggctc ctgcggcaag tttaacgtcg cagtagcatg accacttaca
    72001 ggaagtccat ccggcacgag cggcatagta aattccccca ccagtttaac cggcgactga
    72061 ttttcaaacg cgacgacatc cagttcgctg actgtaagtt gttgcccttt cagctggcct
    72121 tgaaatttaa ctttttcgcc ctgataacgc agttgctgga tatcagaggt taatgcgaga
    72181 gagagttttc cctgccattc ctgccaggga gaaaaaatca gtttatcgat attgatccag
    72241 gtgttaggca gcatggactg ccactgcgcg agggtttttg gagcagctgg cgattgctcc
    72301 gtctgcggca atttcgccag acaagcagaa tcaagttcta ccgtgccgac gttcaataac
    72361 cagcggctgg gatgtgaaag gctggcgttg gtgatatgcg caagctgaca atctcccacc
    72421 agataacgga gatcgggaat gattaaacct ttacgcgtaa tgcgtgggct ttcatctaat
    72481 gcaatacgtg tcccgagcgg tagccagatg cccgccagcg tgggaaccca cagcccgagc
    72541 gtcatcagca gcgtcaacgg cacaagaata atcagtaata acagcgcgag aacggcttta
    72601 tatttaccca gcatgggtag ttaatatcct gatttagcga aaaattaagc attcaatacg
    72661 ggtattgtgg catgtttaac cgttcagttg aaggttgcgc ctacactaag catagttgtt
    72721 gatgaatttt tcaatatcgc catagctttc aattaaattt gaaattttgt aaaatatttt
    72781 tagtagctta aatgtgattc aacatcactg gagaaagtct tatgaaactc gccgtttata
    72841 gcacaaaaca gtacgacaag aagtacctgc aacaggtgaa cgagtccttt ggctttgagc
    72901 tggaattttt tgactttctg ctgacggaaa aaaccgctaa aactgccaat ggctgcgaag
    72961 cggtatgtat tttcgtaaac gatgacggca gccgcccggt gctggaagag ttgaaaaagc
    73021 acggcgttaa atatatcgcc ctgcgctgtg ccggtttcaa taacgtcgac cttgacgcgg
    73081 caaaagaact ggggctgaaa gtagtccgtg ttccagccta tgatccagaa gccgttgctg
    73141 aacacgccat cggtatgatg atgacgctga accgccgtat tcaccgcgcg tatcagcgta
    73201 cccgtgacgc taacttctct ctggaaggtc tgaccggctt tactatgtat ggcaaaacgg
    73261 caggcgttat cggtaccggt aaaatcggtg tggcgatgct gcgcattctg aaaggttttg
    73321 gtatgcgtct gctggcgttc gatccgtatc caagtgcagc ggcgctggaa ctcggtgtgg
    73381 agtatgttga tctgccaacc ctgttctctg aatcagacgt tatctctctg cactgcccgc
    73441 tgacaccgga aaactaccat cttttgaacg aagccgcctt cgatcagatg aaaaatggcg
    73501 tgatgatcgt caataccagt cgcggtgcat tgattgattc tcaggcggca attgaagcgt
    73561 tgaaaaatca gaaaattggt tcgttgggta tggacgtgta tgagaacgaa cgcgatctgt
    73621 tctttgaaga taaatccaac gacgtgatcc aggatgacgt attccgtcgc ctgtctgcct
    73681 gccacaacgt gctgtttacc gggcaccagg cattcctgac agcagaagct ctgaccagta
    73741 tttctcagac tacgctgcaa aacttaagca atctggaaaa aggcgaaacc tgcccgaacg
    73801 aactggttta atcttgccgc tcccctgcat tccaggggag cttattcaga taatccccaa
    73861 agacctttca tcttctattc ttaaaatagc cctgagtcag aaactgtaat tgagaaccac
    73921 aatgaagaaa gtagccgcgt ttgttgcgct aagcctgctg atggcgggat gtgtaagtaa
    73981 tgacaaaatt gctgttacgc cagaacagct acagcatcat cgctttgtgc tggaaagcgt
    74041 aaacggtaag cccgtgacca gcgataaaaa tccgccagaa atcagctttg gtgaaaaaat
    74101 gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa ggcaaactgt ctaatggtga
    74161 actgacagcc aaagggctgg caatgacccg tatgatgtgc gctaacccgc agcttaatga
    74221 actcgataac accattagcg aaatgctgaa agaaggtgca caagtggatc tgaccgcgaa
    74281 ccagttaacg ctggcgaccg cgaaacagac attaacttat aagctggcgg atttaatgaa
    74341 ttaatagctg ccacagctcc cggcggcaag tgactgttcg ctacagcgtt tgccgttggg
    74401 taatgcacac atcccaatcg ccgtaccatc cagttgacgg gcaacagaaa gcgaaccgcc
    74461 gatcattgca caatttgctt ctccactact ggacatcgac gcttttaaac ctggcgctac
    74521 gtgcgccgca gtcgcctgct gaacaggttc actactacac gccgacaaca ataaagcggc
    74581 acaccctacc caaaacgctg ctcgcatctc ttcttcctct gatcttcaag ccaaacgaca
    74641 ccgccataaa taataggcag cacagagggc ggcgtcgaga gctgtcctgc gcgttgcccc
    74701 gcatttttac ttttttatgg ctattttttt gccctctgtt tgatcaaaac attcattacg
    74761 ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc catttgctaa aattgaagga
    74821 ttactactgg gcgcgcagca atttcgtgcg cccctcattt gcgcaatgta agggtgtcat
    74881 atgattacta ttgacggtaa tggcgcggtt gcttcggtgg cgtttcgcac cagtgaagtt
    74941 atcgccatct accctattac ccccagttcc acgatggcag aacaggctga tgcctgggcc
    75001 ggaaacggct tgaagaacgt ttggggagac acaccacgcg tggttgaaat gcagtcggaa
    75061 gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg gtgccctttc aacatcgttt
    75121 acgtcatcgc agggtttgct gctgatgatc ccgacgctgt acaaactggc aggcgaactc
    75181 acaccgtttg tcctgcatgt agcggcacgt accgttgcca cacatgcact ctctattttt
    75241 ggcgatcatt ccgacgtcat ggcggtgcgc cagacgggtt gcgcgatgtt gtgtgcggca
    75301 aacgtccagg aagcgcaaga ctttgctctc atttcgcaca tcgcgacgct gaaaagccgc
    75361 gtgccattta ttcatttctt tgatggtttc cgcacgtccc acgaaatcaa caaaattgtc
    75421 ccgctggccg atgacacgat tcttgatctc atgccgcagg ccgaaattga tgctcatcgc
    75481 gcccgggcac tcaacccgga acatccggtg atccgcggta cgtccgccaa tcctgacact
    75541 tatttccagt ctcgcgaagc caccaaccca tggtacgacg cggtctatga ccatgttgaa
    75601 caggcgatga atgatttctc tgccgcgaca ggtcgtcagt atcagccgtt tgaatattac
    75661 gggcatccgc aagcggaacg ggtgattatc ctgatgggct ctgccattgg cacctgtgaa
    75721 gaagtggttg atgaattgct aatccgtggc gaaaaagtcg gcgtgctgaa agttcgcctg
    75781 taccgcccct tctccgccaa acacttgctg caagctctgc cgggatccgt acgcagcgtg
    75841 gcggtactgg acagaaccaa agaacccggt gcccaggcag aaccgctcta tctggatgtg
    75901 atgaccgcac tggcagaagc ctttaataat ggtgagcgcg aaaccctgcc ccgtgtcatt
    75961 ggtgggcgct atggtctttc atccaaagaa tttggcccgg actgtgtact ggcggtattt
    76021 gccgagctca acgcggctaa accgaaagcg cgctttacgg ttggtattta cgatgatgtg
    76081 actaatctgt cactgccgtt gccggaaaac accctgccaa actcggcgaa actggaagcc
    76141 ttgttttatg gccttggtag tgatggcagc gtttccgcga ccaaaaacaa tatcaagatt
    76201 atcggtaatt ccacgccgtg gtacgcacag ggctattttg tttacgactc caaaaaagcg
    76261 ggcggcctga cggtttctca ccttcgcgtg agcgaacagc cgattcgttc cgcttatctc
    76321 atttcccagg ctgattttgt tggctgccac cagttgcagt ttatcgataa atatcagatg
    76381 gctgagcgtt taaaacctgg cggcattttc ctgctcaaca caccgtacag cgcagatgaa
    76441 gtgtggtcgc gcttgccgca agaagttcag gccgtgttaa accagaaaaa agcgcgcttc
    76501 tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc tggcggcccg tattaatacc
    76561 gtcatgcaga tggctttttt ccatctgacg caaattctgc ctggcgatag cgccctcgca
    76621 gaattgcagg gtgcgattgc caaaagttac agtagcaaag gccaggatct ggtggaacgc
    76681 aattggcagg ctctggcgct ggcgcgtgaa tccgtagaag aagttccgtt gcaaccggta
    76741 aatccgcaca gcgccaatcg accgccagtg gtttccgatg ccgctcctga tttcgtgaaa
    76801 accgtaaccg ctgcgatgct cgccgggctt ggtgacgccc tccccgtttc ggcgctgccg
    76861 ccagacggct cctggccgat gggcactacg cgctgggaaa aacgtaatat tgccgaagag
    76921 atccccatct ggaaagagga actctgtacc caatgtaacc actgcgttgc cgcttgccca
    76981 cactcagcta ttcgcgcaaa agtggtgccg cctgaagcga tggaaaacgc ccctgccagc
    77041 ctgcattcgc tggatgtgaa atcgcgtgat atgcgcgggc agaaatatgt cttgcaggtg
    77101 gctccggaag attgcaccgg ttgtaacctg tgcgtcgaag tttgcccggc gaaagaccgt
    77161 cagaatccag agattaaagc catcaatatg atgtcgcgcc tggaacatgt cgaagaagag
    77221 aaaatcaatt acgatttctt cctcaacctg ccagaaatcg accgtagcaa actggaacgt
    77281 attgatattc gtacatcgca gctgattaca ccgctgtttg aatattcagg tgcttgctcc
    77341 ggttgtggcg agacgccgta tattaaatta ctgactcagc tctatggcga ccggatgttg
    77401 atcgctaacg ccaccggctg ttcttcaatt tatggcggta acctgccctc tacaccgtat
    77461 accaccgatg ccaacggtcg tgggccggca tgggcgaact ctctatttga agataatgcc
    77521 gaatttggcc ttggtttccg cctgacggtc gatcaacacc gtatccgcgt gctgcgtctg
    77581 ctggatcaat ttgccgataa aatcccggcg gaattactga cggcgttgaa atcagacgct
    77641 acgccagagg ttcgtcgaga acaggtcgca gctttacgcc agcaactcaa cgatgttgcc
    77701 gaagcacatg aactgctacg tgatgcagat gcactggtgg aaaaatcaat ctggctgatt
    77761 ggtggtgatg gctgggctta cgatatcggc tttggcggtc tggatcatgt attgagtttg
    77821 acggaaaacg tcaacattct ggtgctggat acgcaatgct attccaacac cggtggtcag
    77881 gcatcgaaag cgacaccgct gggtgcagta actaaatttg gcgagcacgg caaacgtaaa
    77941 gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc atgtttatgt ggcgcagatt
    78001 tctctcggcg cgcagctgaa ccagacggtg aaagcgattc aggaagcgga agcgtatccg
    78061 gggccatcgc tgatcattgc ttatagcccg tgtgaagagc atggttacga tctggcactc
    78121 agccacgacc agatgcgcca actcacagct accggcttct ggccgctata tcgcttcgat
    78181 ccgcgtcgtg ccgatgaagg caaactgccg ctggcgctgg attcacgccc gccgtcagta
    78241 gcactggaag aaacgttact tcacgagcaa cgtttccgtc ggctgaattc gcagcagcca
    78301 gaagtggcag aacagttatg gaaagatgct gcagctgatt tgcaaaaacg ctatgacttc
    78361 ctggcacaaa tggccggaaa agcggaaaaa agcaacaccg attaaatgct ctggataagg
    78421 attatccaat tctaaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
    78481 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaagacata
    78541 taacccgcgc agaataacgt atagaaaatt aa
//
LOCUS       DAPHJE010000005        72372 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPHJE010000005 DAPHJE010000000
VERSION     DAPHJE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.005"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(170..1342)
                     /gene="cgtA"
                     /locus_tag="RH222_000383"
     CDS             complement(170..1342)
                     /gene="cgtA"
                     /locus_tag="RH222_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV3795449.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(1358..2323)
                     /locus_tag="RH222_000384"
     CDS             complement(1358..2323)
                     /locus_tag="RH222_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3795450.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(2450..2707)
                     /gene="rpmA"
                     /locus_tag="RH222_000385"
     CDS             complement(2450..2707)
                     /gene="rpmA"
                     /locus_tag="RH222_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV3795451.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(2728..3039)
                     /gene="rplU"
                     /locus_tag="RH222_000386"
     CDS             complement(2728..3039)
                     /gene="rplU"
                     /locus_tag="RH222_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV3795452.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            3298..4269
                     /gene="ispB"
                     /locus_tag="RH222_000387"
     CDS             3298..4269
                     /gene="ispB"
                     /locus_tag="RH222_000387"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV3795453.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            4496..4774
                     /gene="sfsB"
                     /locus_tag="RH222_000388"
     CDS             4496..4774
                     /gene="sfsB"
                     /locus_tag="RH222_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV3795454.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(4822..6081)
                     /gene="murA"
                     /locus_tag="RH222_000389"
     CDS             complement(4822..6081)
                     /gene="murA"
                     /locus_tag="RH222_000389"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV3795455.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(6136..6390)
                     /gene="ibaG"
                     /locus_tag="RH222_000390"
     CDS             complement(6136..6390)
                     /gene="ibaG"
                     /locus_tag="RH222_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV3795456.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(6550..6843)
                     /gene="mlaB"
                     /locus_tag="RH222_000391"
     CDS             complement(6550..6843)
                     /gene="mlaB"
                     /locus_tag="RH222_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV3795457.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(6843..7478)
                     /gene="mlaC"
                     /locus_tag="RH222_000392"
     CDS             complement(6843..7478)
                     /gene="mlaC"
                     /locus_tag="RH222_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV3795458.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(7497..8048)
                     /gene="mlaD"
                     /locus_tag="RH222_000393"
     CDS             complement(7497..8048)
                     /gene="mlaD"
                     /locus_tag="RH222_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV3795459.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(8053..8835)
                     /gene="mlaE"
                     /locus_tag="RH222_000394"
     CDS             complement(8053..8835)
                     /gene="mlaE"
                     /locus_tag="RH222_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV3795460.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(8843..9652)
                     /gene="mlaF"
                     /locus_tag="RH222_000395"
     CDS             complement(8843..9652)
                     /gene="mlaF"
                     /locus_tag="RH222_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV3795461.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            9862..10839
                     /locus_tag="RH222_000396"
     CDS             9862..10839
                     /locus_tag="RH222_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV3795462.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            10853..11839
                     /gene="kdsD"
                     /locus_tag="RH222_000397"
     CDS             10853..11839
                     /gene="kdsD"
                     /locus_tag="RH222_000397"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV3795463.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            11860..12426
                     /gene="kdsC"
                     /locus_tag="RH222_000398"
     CDS             11860..12426
                     /gene="kdsC"
                     /locus_tag="RH222_000398"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV3795464.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            12423..12998
                     /gene="lptC"
                     /locus_tag="RH222_000399"
     CDS             12423..12998
                     /gene="lptC"
                     /locus_tag="RH222_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV3795465.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            12967..13524
                     /gene="lptA"
                     /locus_tag="RH222_000400"
     CDS             12967..13524
                     /gene="lptA"
                     /locus_tag="RH222_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV3795466.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            13531..14256
                     /gene="lptB"
                     /locus_tag="RH222_000401"
     CDS             13531..14256
                     /gene="lptB"
                     /locus_tag="RH222_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV3795467.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            14304..15737
                     /gene="rpoN"
                     /locus_tag="RH222_000402"
     CDS             14304..15737
                     /gene="rpoN"
                     /locus_tag="RH222_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV3795468.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            15760..16047
                     /gene="hpf"
                     /locus_tag="RH222_000403"
     CDS             15760..16047
                     /gene="hpf"
                     /locus_tag="RH222_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV3795469.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            16165..16656
                     /gene="ptsN"
                     /locus_tag="RH222_000404"
     CDS             16165..16656
                     /gene="ptsN"
                     /locus_tag="RH222_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV3795470.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            16702..17556
                     /gene="rapZ"
                     /locus_tag="RH222_000405"
     CDS             16702..17556
                     /gene="rapZ"
                     /locus_tag="RH222_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV3795471.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            17553..17825
                     /gene="npr"
                     /locus_tag="RH222_000406"
     CDS             17553..17825
                     /gene="npr"
                     /locus_tag="RH222_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV3795472.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            18039..18671
                     /gene="yrbL"
                     /locus_tag="RH222_000407"
     CDS             18039..18671
                     /gene="yrbL"
                     /locus_tag="RH222_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV3795473.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALNSREA"
     gene            complement(18668..19396)
                     /gene="mtgA"
                     /locus_tag="RH222_000408"
     CDS             complement(18668..19396)
                     /gene="mtgA"
                     /locus_tag="RH222_000408"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV3795474.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(19393..20046)
                     /gene="elbB"
                     /locus_tag="RH222_000409"
     CDS             complement(19393..20046)
                     /gene="elbB"
                     /locus_tag="RH222_000409"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV3795475.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(20276..22612)
                     /gene="arcB"
                     /locus_tag="RH222_000410"
     CDS             complement(20276..22612)
                     /gene="arcB"
                     /locus_tag="RH222_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV3795476.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(22708..23637)
                     /locus_tag="RH222_000411"
     CDS             complement(22708..23637)
                     /locus_tag="RH222_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV3795477.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            24312..28772
                     /gene="gltB"
                     /locus_tag="RH222_000412"
     CDS             24312..28772
                     /gene="gltB"
                     /locus_tag="RH222_000412"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV3795478.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            28785..30203
                     /gene="gltD"
                     /locus_tag="RH222_000413"
     CDS             28785..30203
                     /gene="gltD"
                     /locus_tag="RH222_000413"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV3795479.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            30387..31514
                     /locus_tag="RH222_000414"
     CDS             30387..31514
                     /locus_tag="RH222_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV3795480.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(31574..32038)
                     /locus_tag="RH222_000415"
     CDS             complement(31574..32038)
                     /locus_tag="RH222_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV3795481.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(32035..32910)
                     /gene="nanK"
                     /locus_tag="RH222_000416"
     CDS             complement(32035..32910)
                     /gene="nanK"
                     /locus_tag="RH222_000416"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV3795482.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(32907..33596)
                     /locus_tag="RH222_000417"
     CDS             complement(32907..33596)
                     /locus_tag="RH222_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV3795483.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(33644..35134)
                     /gene="nanT"
                     /locus_tag="RH222_000418"
     CDS             complement(33644..35134)
                     /gene="nanT"
                     /locus_tag="RH222_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV3795484.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(35243..36136)
                     /gene="nanA"
                     /locus_tag="RH222_000419"
     CDS             complement(35243..36136)
                     /gene="nanA"
                     /locus_tag="RH222_000419"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV3795485.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(36258..37049)
                     /gene="nanR"
                     /locus_tag="RH222_000420"
     CDS             complement(36258..37049)
                     /gene="nanR"
                     /locus_tag="RH222_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV3795486.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            37429..38796
                     /gene="dcuC"
                     /locus_tag="RH222_000421"
     CDS             37429..38796
                     /gene="dcuC"
                     /locus_tag="RH222_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV3795487.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(38839..39336)
                     /gene="sspB"
                     /locus_tag="RH222_000422"
     CDS             complement(38839..39336)
                     /gene="sspB"
                     /locus_tag="RH222_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV3795488.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(39342..39980)
                     /gene="sspA"
                     /locus_tag="RH222_000423"
     CDS             complement(39342..39980)
                     /gene="sspA"
                     /locus_tag="RH222_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV3795489.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(40375..40767)
                     /gene="rpsI"
                     /locus_tag="RH222_000424"
     CDS             complement(40375..40767)
                     /gene="rpsI"
                     /locus_tag="RH222_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV3795490.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(40783..41211)
                     /gene="rplM"
                     /locus_tag="RH222_000425"
     CDS             complement(40783..41211)
                     /gene="rplM"
                     /locus_tag="RH222_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV3795491.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(41430..42557)
                     /gene="zapE"
                     /locus_tag="RH222_000426"
     CDS             complement(41430..42557)
                     /gene="zapE"
                     /locus_tag="RH222_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV3795492.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMMRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            42751..43149
                     /locus_tag="RH222_000427"
     CDS             42751..43149
                     /locus_tag="RH222_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV3795493.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            43303..44670
                     /gene="degQ"
                     /locus_tag="RH222_000428"
     CDS             43303..44670
                     /gene="degQ"
                     /locus_tag="RH222_000428"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV3795494.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDYVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            44760..45827
                     /gene="degS"
                     /locus_tag="RH222_000429"
     CDS             44760..45827
                     /gene="degS"
                     /locus_tag="RH222_000429"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV3795495.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(45889..46827)
                     /gene="mdh"
                     /locus_tag="RH222_000430"
     CDS             complement(45889..46827)
                     /gene="mdh"
                     /locus_tag="RH222_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV3795496.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            47262..47732
                     /gene="argR"
                     /locus_tag="RH222_000431"
     CDS             47262..47732
                     /gene="argR"
                     /locus_tag="RH222_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV3795497.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            48097..48360
                     /gene="yhcN"
                     /locus_tag="RH222_000432"
     CDS             48097..48360
                     /gene="yhcN"
                     /locus_tag="RH222_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV3795498.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(48416..48688)
                     /locus_tag="RH222_000433"
     CDS             complement(48416..48688)
                     /locus_tag="RH222_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV3795499.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(48780..50747)
                     /gene="aaeB"
                     /locus_tag="RH222_000434"
     CDS             complement(48780..50747)
                     /gene="aaeB"
                     /locus_tag="RH222_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV3795500.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(50753..51685)
                     /gene="aaeA"
                     /locus_tag="RH222_000435"
     CDS             complement(50753..51685)
                     /gene="aaeA"
                     /locus_tag="RH222_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV3795501.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(51693..51896)
                     /locus_tag="RH222_000436"
     CDS             complement(51693..51896)
                     /locus_tag="RH222_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV3795502.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            52079..53008
                     /gene="aaeR"
                     /locus_tag="RH222_000437"
     CDS             52079..53008
                     /gene="aaeR"
                     /locus_tag="RH222_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV3795503.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(53136..54581)
                     /gene="tldD"
                     /locus_tag="RH222_000438"
     CDS             complement(53136..54581)
                     /gene="tldD"
                     /locus_tag="RH222_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV3795504.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(54737..58537)
                     /gene="yhdP"
                     /locus_tag="RH222_000439"
     CDS             complement(54737..58537)
                     /gene="yhdP"
                     /locus_tag="RH222_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV3795505.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(58605..60074)
                     /gene="rng"
                     /locus_tag="RH222_000440"
     CDS             complement(58605..60074)
                     /gene="rng"
                     /locus_tag="RH222_000440"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV3795506.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(60064..60657)
                     /gene="yhdE"
                     /locus_tag="RH222_000441"
     CDS             complement(60064..60657)
                     /gene="yhdE"
                     /locus_tag="RH222_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV3795507.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(60666..61154)
                     /gene="mreD"
                     /locus_tag="RH222_000442"
     CDS             complement(60666..61154)
                     /gene="mreD"
                     /locus_tag="RH222_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV3795508.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(61154..62257)
                     /gene="mreC"
                     /locus_tag="RH222_000443"
     CDS             complement(61154..62257)
                     /gene="mreC"
                     /locus_tag="RH222_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV3795509.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLALRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(62323..63366)
                     /gene="mreB"
                     /locus_tag="RH222_000444"
     CDS             complement(62323..63366)
                     /gene="mreB"
                     /locus_tag="RH222_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV3795510.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(63671..65611)
                     /gene="csrD"
                     /locus_tag="RH222_000445"
     CDS             complement(63671..65611)
                     /gene="csrD"
                     /locus_tag="RH222_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV3795511.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            65763..66737
                     /locus_tag="RH222_000446"
     CDS             65763..66737
                     /locus_tag="RH222_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3795512.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            67715..68185
                     /gene="accB"
                     /locus_tag="RH222_000447"
                     /gene_synonym="fabE"
     CDS             67715..68185
                     /gene="accB"
                     /locus_tag="RH222_000447"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV3795513.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            68196..69545
                     /gene="accC"
                     /locus_tag="RH222_000448"
     CDS             68196..69545
                     /gene="accC"
                     /locus_tag="RH222_000448"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV3795514.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            69654..69896
                     /locus_tag="RH222_000449"
     CDS             69654..69896
                     /locus_tag="RH222_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV3795515.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            69886..71337
                     /gene="panF"
                     /locus_tag="RH222_000450"
     CDS             69886..71337
                     /gene="panF"
                     /locus_tag="RH222_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV3795516.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLVAFLVGNRFGTSVPQATVLTTDK"
     gene            71349..72230
                     /gene="prmA"
                     /locus_tag="RH222_000451"
     CDS             71349..72230
                     /gene="prmA"
                     /locus_tag="RH222_000451"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV3795517.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT        17661 a        19451 c        18169 g        17091 t
ORIGIN      
        1 gcatatggcc ggggtttcag attattgaca aagtgcgctt tgtttatgcc ggatgcggcg
       61 tgaacgcctt atccggccta caaaatcgtg caaattcaat atattgcaat catcttgtag
      121 gcctgataag tgtagcgcat caggcttatt tggcattggt cattagtgat taacgcttgt
      181 aaatgaactc aacgccttct tcgtcgtctt cgtcccagtc gtcatcccag tcctcatcat
      241 cctcttcagc aatctcttca agctgctggc gatgataatc atcccacatg aattcgactt
      301 tctctggctg tttcgcttct tcagcctgca cgaccgggtt ttcaatgata aaggtcatca
      361 catcccagca gagatctttc acgcccagtc cgctcgccgc agagatcaga taatatttat
      421 cttcccagcc cagcgcctca gcgatcgctt tcgctttctc ttcggcttct gccttatcca
      481 gcagatcgat cttgttgaac actaaccaac gcggtttcgc cgccagatcc tggctgtatt
      541 tttccagctc gctgataata atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat
      601 cgatatcgat gaggtgcaac aggacgcggc aacgttccag gtgcttcagg aagcggatac
      661 ccagacctgc gccttccgca gcgccttcaa tcagtcctgg aatatcggca acaacgaagc
      721 tcttttcgtt gtccattcgt accacaccca gacttggcac cagagtggta aacggataat
      781 ccgccacttt cggtttagcc gccgataccg cgcgaataaa ggtcgatttc cccgcgtttg
      841 gcatccccaa cataccgacg tcagccagca gcatcagctc cagcagcagc tcacgcttat
      901 cacccggcgt accgttggtt ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg
      961 tattgcccag accgtgccag ccgcccttag caaccagcag acgctgaccg tgtttagtca
     1021 tatcgcccat ggtttcacca gtaccctggt cgattacacg cgtacctacc ggcactttaa
     1081 tcgtgacgtc tttaccgcgc ttaccggtac agtcgcggct tgcgccgttt tgaccgcgct
     1141 ctgcacggaa agatttttca aaacgataat cgataagcgt gttcaggttc tcgtcggctt
     1201 ccatccatac gtcaccacca tcaccgccgt cgccgccatc cgggccgcct ttcggaatat
     1261 acttttcgcg gcggaagctc acgcaaccat taccgccatc acctgcaacg accagaatcg
     1321 atgcttcatc aacaaacttc attttattct ccgtaaatca ttcgcctgcg cggggttgcg
     1381 ataccaccgt tgtatgctta cgtaatccgc cccaaatacg atgaccaatg gcggaataca
     1441 tcgcgcccgc aaccacgaca aacgcaccga gataacctaa aaggtttaac atcggtctgg
     1501 cgaagaaatc gggccaggcc agtgataaaa gatctgaaaa aaacagcgta aacagtgggg
     1561 tgagcgtgat gatcgcgctc acctgcgctg cctgccagcg agccatcgct tccgccaggg
     1621 cgccatatcc taccaatgta ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc
     1681 taagctgcgc tatcactcca ggctttgcca gagggaagag cgcgattgta cataaagtgt
     1741 acagtaaaaa cagaatctgc ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc
     1801 cataactcac ccaaaccgtc gccgcaccga ccccaaagat aactccccag gtgtaatcgg
     1861 tgagctttgt aaatatctcg accagactgg tgttaaaaaa catcaccagg ccgctcagga
     1921 gcatcaatgc ccctacaacc tgagtgctgc gcattttctc tttcaggata aatacgctgg
     1981 caaccatcat gccaactggc gagagttgcc caatcacctg cgaagcggtc ggactcaggt
     2041 attgcaagga tgagctgaac aggataaagt tcccaaacag cccggcggtc gccactgcca
     2101 acaaaatcaa ccagcgtggc ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg
     2161 caagaatggc accaaggcca atactcgcca tcaagaaacg gtaaaacacg attgtcggag
     2221 gttccatcac ctccagcacc tgcttcattg cgattggcaa cgccccccag caaattgctg
     2281 tggtgagcgc caaaagaatg ccaatgcctg cctgctgctt cataccgtat tccctgcaaa
     2341 gcgcatttgc ggattaacgt cgcaccatgc tcatttttac cggttatcga atgtaaaaag
     2401 ccccgcaacg tgttgcgggg ctttcatccg ttaccgggac gcgaaaaact tattcagctt
     2461 cgatgctgat aaatttacgg tttttcgggc ctttaacttc gaatttcact ttaccgtctg
     2521 ctttagcaaa cagagtgtgg tcacgaccgc aacctacgtt agcgccagcg tggaatttgg
     2581 taccacgttg acgaacgatg atgctacccg ccagaacgga ttcgccaccg aaacgcttaa
     2641 cgcccaggcg tttagcttct gaatcgcgac cgttacgtgt ggagccgcca gcctttttat
     2701 gtgccatttg aaatctctcc tcaggtctta ggcgctgatg ccagtaattt tcacatcagt
     2761 gaaccactga cgatggccct gctgcttacg atagtgttta cgacgacgaa acttaacgat
     2821 tttaactttc tcgccacgac cgtgagcaac aacttcagct ttgattacgc cgccatcaac
     2881 gaaaggaacg ccgattttga cttcttcacc gtttgcgatc atcagcactt cagcgaactc
     2941 aacagtttcg ccagttgcga tgtccagctt ttccaggcga acggtctgac cttcgcttac
     3001 tcggtgttgt ttaccaccac tttggaaaac cgcgtacata taaaactccg cttccgcgca
     3061 caccttttca atgattcaga gtgcgctata aatattcaca atagggcgcg aatattacgc
     3121 aaaacgcacg cctttgacaa gtgctacagt caatacacga agaaaaaaaa cacaacttgt
     3181 acggtaacgt ttatctgtgc cattttttca gtacaatcac cctatattcc taaccataaa
     3241 ccctaagttg cctttgttca cagtaaggta atcggggcga aaagcccggc ttttgcgatg
     3301 aatttagaaa aaatcaatga gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc
     3361 cttgagcagc ttaattccga cgtccaactg atcaatcagt taggctatta catcgtcagc
     3421 ggcggcggta aacgtattcg tccgatgatt gctgtactgg ctgcacgagc tgttggctat
     3481 gagggaaatg cgcatgtcac cattgctgcc ttgatcgagt ttatccacac ggcgactctg
     3541 ctacacgacg acgttgtgga tgaatcagat atgcgcaggg gtaaagctac cgccaacgct
     3601 gcatttggca atgccgccag cgtgctggta ggcgatttta tttatacccg cgcgttccag
     3661 atgatgacca gcctcggttc gctcaaagtg ctggaagtca tgtcagaagc cgtaaacgtc
     3721 atcgcagaag gtgaagttct gcaactgatg aacgttaacg atccggacat cactgaagaa
     3781 aactacatgc gcgttatcta tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt
     3841 tccgggattc tggctggctg tacgccggag gaggagaaag gcctgcagga ttatgggcgc
     3901 tatctcggca ctgctttcca gttgatcgac gatttactcg attacaatgc cgatggcgaa
     3961 cagttaggta aaaacgtcgg cgacgatctg aacgaaggta aaccgacgct gccgctgctg
     4021 catgcgatgc atcatggcac accagaacag gcacagatga tccgtaccgc catcgaacag
     4081 ggtaacggtc gccatcttct ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt
     4141 gaatggacgc gtcagcgcgc cgaggaagaa gcagacaaag ccatcgcagc gttacaggtg
     4201 ctcccggaca ccccttggcg agaagcactc atcggcctcg cgcacatcgc tgttcaacgc
     4261 gatcgttaat cccctcccct catcccgcgc agcgcgcggg gtggttccag taaattccat
     4321 aaaaacactt actcagctct ataacacctg aaaaacaggc tgtgcatctt tatatattct
     4381 gaatattcac actccttaca tgaacttttt agagcaatcg gccatcagga gtatagtgat
     4441 gctcgacaga agaagtgttc tgaatgaaag cgactaactt aaggagtgag gaaagatgga
     4501 aagtaatttc attgactggc atcccgctga catcattgcg ggtttgcgca agaagggaac
     4561 gtcaatggcg gcggagtctc gcagaaatgg tttgagttcc tcaacgctgg cgaatgcatt
     4621 atcgcgccca tggccgaaag gagagatgat tattgcgaaa gccctgggaa ctgacccctg
     4681 ggttatctgg ccatcacgct accatgatcc gcagacccat gagtttatcg acagaacgca
     4741 gttgatgcgt agctacacta aaccgaaaaa atgagtggtc tggcggtagc cccgcgaacg
     4801 gggctgccag ctctcagacg attattcgcc tttcacacgc tcaatatttg cacctaaagc
     4861 gcgcagtttg tcttcaatgc gttcgtagcc acgatcgatg tgataaatac gatcaaccac
     4921 cgtcgtccct tccgcaatac agccagccag caccaggctt gctgatgcac gcagatcggt
     4981 tgccataacc tgtgcgccag aaagtttttc aacaccgtga caaataacgg tattgctttc
     5041 gatttcggca tgcgcgccca tacggctaag ctccggcaca tgcataaagc ggttttcaaa
     5101 gaccgtttcg gtgatgaatc cggtcccttc tgccaccagg ttcaacagcg tgaactgggc
     5161 ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt acgttaacag ccttcggacg
     5221 tttgccatgc atatccaggc taatccagtc ttcgccgact tcgatgtccg ctccagcatc
     5281 acgcagtttc gccagcacgg cgtccagagt atctggctgc gcgttacggc agataatttt
     5341 gccgcgagaa atcgccgccg ccaccaggaa agtaccggtt tcgatacgat ccggtagcac
     5401 gcgatagaca ccgccgccta aacgttccac accttcgatg acgatacgat cggtgccctg
     5461 accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg gtatcgacga tttccggttc
     5521 acgcgctgcg ttttcaataa tcgtggtacc ttccgccagg gttgcagcac acatgatggt
     5581 caccgttgcg ccaacgctga ctttatccat cacgatatgt gcgcctttca gacgaccatc
     5641 gacggaagct ttaacgtaac cttcttccag tttgatggtc gcgcctaatt gttcgaggcc
     5701 agaaatgtgt agatcaaccg gacgcgcacc gatcgtacaa ccgccaggca gtgaaacttg
     5761 cccctgacca aagcgcgcta ccagcggccc cagtgcccag atagaagcac gcatggtttt
     5821 aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg gcatcaatat gcacagaacc
     5881 attacgttct actttcgcac ccagctggct tagcagcttc attgatgtat cgacgtcttt
     5941 cagtttcggg acgttctgga tctctaccgg ttcttccgcc agtagagcgg caaaaaggat
     6001 aggcagagca gcatttttag cgccggaaat tgtgacttcg ccctggagct tcgttggccc
     6061 ctgaacacga aatttatcca tttagtttgt tctcagttaa caattcatat ccgctaccgg
     6121 cgaatcgccc atagctcaaa agccgttcag tttgcgatcg cgcgcccact ccgcaggggt
     6181 atacgctttg atcgacacag catgaatgcg gttatccgca atatattcca tcagcgggcc
     6241 atagaccgtc tgctgttttt taacccgact catgccgtca aacaactcac ccacggcaat
     6301 aacctgaaag tggctgccat cgccggaaac gtggacttcc tggagggaga gagcgttcat
     6361 caacacgctc tgaatttcat tattttccat gggatcttca atcatcagtt aataaaccag
     6421 cgaaacatct tagagcaaag ttgcgctggc ataaataagc aaaaagcctc gctgataaat
     6481 cagacaaggc tcgacttgca ggcaggtttg ccggacaggc ggttaacgcc atatccggcc
     6541 tgaaaaaatt taacgaggca gaacatcagc aggcaaatta tacaatttcg ccagggtata
     6601 cactttgtcg tttaccccct gaagcgtcac attgttgccc tgctttttcg ccagatcgat
     6661 aagatggagc agcagtgcca gtccccccgt atccacgcgg gagacacggc taagatcgat
     6721 gcaggtaatc cccttcaccg cttcctcacg catttcccaa agcggtagca aaacgtcctg
     6781 atccagctct ccggataacg ccagcgtgtc acccgtctgc atccagctca gtgactcgct
     6841 cattattttt tctcttccag agtgattttc tgttgagaaa tcgatttcag ttgcgctgtc
     6901 aggccgtcga tacctttggt acgcagcagc gttccccact cgttttgttt ggtggtgatc
     6961 atactgacgc cttcagcaat catgtcgtaa gcctgccagt tgcccgtctg ggagttttta
     7021 cgccactgga agtccagacg caccggcgga cggccattcg ggtcaataat ggtaacgcga
     7081 ataggcacaa tggttttatc gcccagcggc tgttctggcg caatctgata ggtttgaccg
     7141 tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt actcacggaa agcggcaaag
     7201 taggcttcac gttgagcagg ggtcgcactc ttgtaatact ggcccagcac cagcgcaccg
     7261 gcgtatttca cctgtacgta tggcagcagt tcctgatcaa caatggtacg cagataatcc
     7321 gggttggccc gaatttgcgg ttgctcattc ttcaggcgat cgaacgtttt ctgcgccgcc
     7381 tcgtccatca gcttatacgg attggtctgg tctgccgcgg ttgccgcact cagaggtgca
     7441 atcaccagca aagcgaccat cattaaacgt ttaaacatgc gtcggttctc ctgaaattat
     7501 ttcgttgtac ccacaggttc agtggtttca ttattacctg gcgcagcagc tggcgcatcg
     7561 ccactattct tattgtcatc gcctttacta ccgtaaagga actgaccaat gagatcttcc
     7621 agcaccatcg cagacttagt gtcctgaatt gtatcgccat ccttcaggat agcagtcccc
     7681 agttccgggt cttcaaaacc gacgtttaat gccagatatt gttcccccag caggccggaa
     7741 gtacgaatgc tcagcgaact ggtatctgga atgtggttat aacgttgttc aatttccagc
     7801 gttacgcgcg gcagataggt tttcgggtcc agcgtaatat ccgccacccg acccacaaca
     7861 acgccaccaa tactgaccgg agagcgcgct ttcaggccgc caatgttatc gaacgtcgca
     7921 taaagcgtgt aggtcggttc agtacgtatg gacgtcacgt tcgccgcctt caggcaaaca
     7981 aacagcgccg ccagcagtgc tgctaataaa aagataccca cccaaatttc attttttttc
     8041 gtttgcatga actcaattcc caaacatcaa tgcggtcagc acaaaatcca gccccagaac
     8101 agccagagac gagtggacaa cggtgcgagt ggttgcccgg ctaatcccgg cagacgtcgg
     8161 gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc gtgatggcga acaccacgct
     8221 cttaatcaga cagttgacca gatccatacg ccagtcgacg gcattttgca ttgccgacca
     8281 gaagaacccg ctatcaatgc ctttccaact gacgccgacc agagatccgc cccagatccc
     8341 cacggcgacg aaaataaccg tcaacagtgg taatgaaata accccagccc agaaacgggg
     8401 agaaataacc cgacgcagcg gatccaccgc catcatctcc atactggaga gttgctctgt
     8461 agcgcgcatc aggccgattt ctgcggttag cgccgaacca gcacgcccgg caaacaacaa
     8521 cgcggcaacc accggcccca gttcacgcag tagcgataac gccaccagca tacccagact
     8581 ggtttccgca ctataagtgg tcagaaccag ataaccttgc agccccaaca ccattccgat
     8641 gaacacgcca gaaaccacaa taatcagcat cgacaggacg ccgacattat agagctggcg
     8701 caccagcagc ggcgcatgtt tgcgaaattc cggtttgccg accagcgcat tgaataacat
     8761 taacccggcc cgcccgaacg ttctcagggt tttaatccct ttatgtccga gcgacgccag
     8821 cgcatttaac agcatgagtg gcttaactcc ctggtaaaag atcagcgtga taatcgccgg
     8881 caggatagcg gaacggaaca ggcccgtcag ctatcccgtc cagaaactga cgtacgcgcg
     8941 gatcaggatt cgcctgcaac gcctgggcac tgccatgagc gacaattttt ttgtccgcca
     9001 ggatccaggc gtgatccgca atacttaaca cttccggcac atcgtgagaa accaccacac
     9061 aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt caccagtacg cccatggtga
     9121 tgggatcttg cccaacaaaa ggttcatcaa acatgatgag atccggctcc agcgcaatcg
     9181 cacgcgccag cgctgcgcgc cgcgccatcc caccggaaag ttcagaaggc attagtttag
     9241 ccgctccacg cagccccacg gcctccagct tcatcatcac cgtactatgc aacaatggcg
     9301 cgggaagttg ggtatgttcg cgcagtggat aggcgacgtt gtcaaatacg ttcatatcag
     9361 tgaacaacgc cccggactga aataacatgc tcatccgttt gcgcactgta tacaggcgcg
     9421 aacgagacat cgccggaata ttctcaccat cgaaaaggat ctcaccatga tctggtgcga
     9481 tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat gcccgatggc cccatgatcg
     9541 ccgtgatctt ccctcgcggc acggtcaggg aaatattatc gaagatgcag cgattgccac
     9601 gcgtaaaact gacatcgcgc atatcgacta aattcgccac agactgctcc ataattcacc
     9661 cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt aaccttgaac ccaacatatt
     9721 tacagaatat tacccgccgt ggttagcgaa agctggcatt tgttttactt tttagccgca
     9781 taaagtcaaa attaagaatc cgttacggct ttctgaaaat cttcagcgga ccggcgagta
     9841 tacctgaaga aaggacgtta gatgctttta gctacggcac tgttaattgt tggtttactt
     9901 ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt ctattctttg ccgaaccttt
     9961 ggcatcccgc cgctgatcat cggcatgacg gtggtcagta ttggtacatc gttaccagaa
    10021 atcatcgtct cgcttgccgc gtctctgcac gaacaacgcg atttagccgt tggtacagcc
    10081 ctcggctcaa acattatcaa tatattgctg atcctcggtc tggctgcgct ggttcgtcct
    10141 tttaccgtcc attctgatgt tctacgccgt gaattaccct taatgttgtt ggtcagcgtg
    10201 gtggccggtt ccgtactcta tgacggacaa cttagtcgaa gcgatggtat ctttctcctc
    10261 tttctggctg tgctatggct gctgttcatt gttaaacttg cacgtcaggc tgaacgtcag
    10321 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    10381 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    10441 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatgggtctg
    10501 acggcaattg ctatcggaac cagcctgccg gaactggcaa ccgcaatagc gggggttcgc
    10561 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    10621 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    10681 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    10741 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    10801 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    10861 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    10921 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcttgtga
    10981 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    11041 cgggcgcaaa atggcggcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    11101 tggtgaagcc gcgcatggtg atttaggcat ggttacccca caggatgtgg tgattgctat
    11161 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    11221 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    11281 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    11341 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    11401 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    11461 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    11521 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    11581 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    11641 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    11701 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    11761 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    11821 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    11881 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    11941 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    12001 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    12061 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    12121 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    12181 gatttgctgg aaaaactggc gattgccccg gaaaatgtgg cttatgtcgg cgatgatctc
    12241 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    12301 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    12361 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    12421 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    12481 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    12541 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    12601 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    12661 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    12721 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    12781 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    12841 ctcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    12901 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    12961 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    13021 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    13081 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    13141 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    13201 ggcgaacaag gtaaagaagt gattgacggc tacggtaaac cggcaacgtt ctaccagatg
    13261 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    13321 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    13381 gataagatca cttacctggt aaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    13441 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggata aaaacaacaa aggccagacc
    13501 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    13561 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    13621 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta tatggttgta
    13681 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    13741 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    13801 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    13861 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    13921 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    13981 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg tgttgacccg
    14041 atctcggtta tcgacattaa acgcatcatt gagcacctgc gcgacagcgg cctgggcgtg
    14101 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    14161 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    14221 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    14281 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    14341 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    14401 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    14461 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    14521 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    14581 ccgctggtac accatccggc accagcggtg actacattga cgatgagctg ccggtctatc
    14641 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    14701 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gacaccggtt
    14761 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    14821 acgaggttga agccgtcctt aagcggatcc aacggtttga tccggtcggt gtagcggcaa
    14881 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataaaacc acgccgtggc
    14941 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    15001 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    15061 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    15121 ttccagatgt gctggtgcgt aagcataacg gtcactggac agtagaactc aacagtgaca
    15181 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gctcgcaatg
    15241 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    15301 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    15361 ccttctttga gcaaggcgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    15421 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    15481 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    15541 gcgaagcttc ctccacggcg attcgtgcgc tggtgaagaa attaatcgcg gcggaaaacc
    15601 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    15661 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    15721 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    15781 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagccaaatt tgccaaactt
    15841 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    15901 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    15961 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    16021 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    16081 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    16141 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    16201 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    16261 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    16321 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    16381 catggcaaac tggaagaaga tactctgcgc gccgttggcg ttttcgttca gctcgaaacg
    16441 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    16501 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    16561 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    16621 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    16681 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    16741 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    16801 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    16861 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    16921 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    16981 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    17041 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    17101 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    17161 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    17221 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    17281 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    17341 gaagtacaca attttatcta ccagacgcga agctatcttg aactatggtt acctatgctg
    17401 gaaaccaaca accgtagcta cttgacggtc gccattggtt gtaccggcgg aaagcaccgt
    17461 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    17521 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    17581 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    17641 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggccgaagcc aacagcgtta
    17701 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggcc
    17761 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taactctggt tttgatgaag
    17821 attaatcttc atcactttga catacaactc ccttcaaact ccccccgaca ataagaaaat
    17881 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    17941 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    18001 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    18061 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    18121 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    18181 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    18241 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgca gattttgacg
    18301 gcaaaccctc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    18361 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    18421 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    18481 cggtcgtctg tgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    18541 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    18601 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcaattcgc
    18661 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    18721 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    18781 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    18841 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    18901 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    18961 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    19021 ttttggccgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    19081 tgtgcgccag ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    19141 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    19201 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga
    19261 ccattaccgc tgagaaggga acaggcgcaa cactaaacaa cgcgatgccc ccgccccaga
    19321 aaacggcgag gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta
    19381 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    19441 cgccgcttct gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc
    19501 ttcatcaacc acgatatcat cgacaggaca gggcacatgc tccgcgccca tctcttccag
    19561 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    19621 cggcagcatc gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc
    19681 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagag tggcaaaatt
    19741 gcttaaattc ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc
    19801 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    19861 gttgcgcgtt tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt
    19921 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    19981 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    20041 cttcattgtg atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    20101 gtacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    20161 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc
    20221 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    20281 ttttagtggc ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt
    20341 tcatctcttc aatccattca ccgacgttat cttcccaggc aggaaggtcc ggagactgaa
    20401 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    20461 tatgtccttc ctctacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    20521 tgacatagcc cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    20581 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    20641 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    20701 tcgcggttag cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga
    20761 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    20821 cgcgcggata acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact
    20881 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    20941 cggtcatggc gacatcaacg ctgttaccta atttttccag cacagaacgc gcaacaatca
    21001 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    21061 catcaaacgc atcatcgacc tcttctgcta ccgacggtgc gtggatcgtc aacgtaaagg
    21121 ttgaaccttt gccctgttcg ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    21181 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    21241 tcacctggta atacatggcg aaaattttat ccagctcatc ctgcggaatg ccgataccag
    21301 agtcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    21361 ggccttgctg ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac
    21421 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt cggctccagg ttaaagcgca
    21481 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    21541 aatcaaccgg ttgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    21601 tatcgttaaa gatattcccc agcgtgacgg ccgaaacatg gatggtcttg agatattttt
    21661 cctgctcggc ggtgagttcg gtatccagca gaatacggct caggccgacg ataccgttca
    21721 gcggtgtacg caattcgtga ctgatggtgg agataaacgt cgttttgtcg cggctggccc
    21781 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    21841 cgtgacgttt acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc
    21901 gcccgtccgg gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt
    21961 tttcatcggt ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca
    22021 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    22081 aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg tgaagcatca aggaaggaac
    22141 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    22201 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcgattt
    22261 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    22321 gcatctcctc cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct
    22381 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    22441 aacgaataac atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta
    22501 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    22561 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    22621 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    22681 gagaaatgcg gaattcccct caacgaccta ctccgttgga ggtatccacg gacgttccag
    22741 cgccgatccc tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca
    22801 tcgattttcg caccacagag gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    22861 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtgtaatc
    22921 ctccagttca ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    22981 cacaatatgc agcggatgca gctttatgcc gtccacgccg gtttcaacca ctcgctccag
    23041 cgtttgcaaa cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac
    23101 cttcagcccg cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc
    23161 gcggttgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    23221 ttcgtaaccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    23281 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    23341 agaacgcagc acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata
    23401 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    23461 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat
    23521 ggtaccgtca cggttagggc agctaaaacc gccgtgtagc gtcagcttat gcaccttttg
    23581 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    23641 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat
    23701 gacctggatc aatcgtcccg gcccgctttt atcaactgca taatcaatca aaattaccga
    23761 aatttcatgc ataatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    23821 cttatggatt ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa
    23881 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    23941 aggaattgac tgtttctcta acgacttccc ttttagcctt aaagataaaa tccattttaa
    24001 tttcagtaat ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    24061 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    24121 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    24181 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    24241 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    24301 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    24361 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    24421 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
    24481 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    24541 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    24601 ccgcacgccg catcgttgaa gaagagctgc aacgcgaaac cttgtcgatt gtgggctggc
    24661 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccccaca
    24721 ttgagcaaat tttcgttaac gctccggcag gctggcgtcc gcgcgatatg gagcgccgtc
    24781 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    24841 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
    24901 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catctgcctg ttccaccagc
    24961 gcttctccac taacaccgta ccgcgctggc cgctggcgca gccgttccgc tacctggcgc
    25021 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
    25081 aattccagac gccgctgatc cctgatctgc acgacgccgc accgttcgtc aacgaaaccg
    25141 gttctgactc cagttcgatg gataacatgc tggaactgct gctggcaggc gggatggata
    25201 tcatccgcgc catgcgtcta ttagtaccac ccgcctggca gaacaacccg gatatggacc
    25261 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggcccgg
    25321 cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
    25381 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
    25441 tctgggatta tcagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
    25501 tggtgatcga cacccgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    25561 aaagccgcca tccatataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    25621 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    25681 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
    25741 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
    25801 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
    25861 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
    25921 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
    25981 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
    26041 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctgt
    26101 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct gctggtgctc tccgaccgga
    26161 atatcgctaa agatcgcctg ccggttccag ccccgatggc ggttggcgcg atccagaccc
    26221 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    26281 gcgatccgca ccacttcgcc gtgttgctgg gcttcggcgc gacggctatt tatccatacc
    26341 ttgcctatga aacgctgggc cgcctggtag acacccatgc gattgccaaa gattatcgta
    26401 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    26461 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctgc
    26521 acgatgatgt agtgggcctg tgcttccagg gggcggtcag ccgcattggt ggggcgagct
    26581 ttgaagactt ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc
    26641 ccatcagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcctacaacc
    26701 cggacgtggt gcgcacgctg caacaggcgg tacagagcgg cgagtatagc gactatcagg
    26761 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    26821 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    26881 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    26941 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
    27001 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
    27061 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    27121 agccgggcga aggcggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
    27181 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
    27241 tcgaggactt agcgcagctc attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    27301 ccgtgaagct ggtttccgaa ccgggcgtag gcaccatcgc gactggcgtg gcaaaagctt
    27361 atgccgattt aatcaccatc gcaggctatg acggtggcac cggcgcaagc ccgctgtcgt
    27421 cagtgaaata cgcaggttgc ccgtgggagc tggggcttgt tgaaacccag caggcgctgg
    27481 ttgccaatgg tctgcgtcat aagatccgtt tgcaggtcga tggcggcctg aaaaccggtg
    27541 ttgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccga
    27601 tggtggcgct cggctgtaaa tatctacgta tttgccatct gaacaactgc gcaacgggtg
    27661 tagcaactca ggatgacaaa ctgcgtaaga accactatca cggcctgccg ttcaaggtta
    27721 ccaattactt tgaatttatt gcccgtgaaa cccgcgagct gatggcacag ctgggcgtaa
    27781 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agaactggac ggtttcaccg
    27841 ccaaacagca aaaactggcg ctgtcgaagc tgctggagac cgccgaaccg catccaggta
    27901 aggcactcta ctgcaccgaa aacaacccac cgtttgataa cggcctgctg aacgcgcagt
    27961 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
    28021 ttcgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
    28081 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
    28141 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
    28201 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
    28261 tccgcagcca tgaagcaagt attatcggca acacctgcct gtatggcgcg accggtggtc
    28321 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca
    28381 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt attgtctgta
    28441 ttctgggtaa aaccggcgtt aacttcggtg cgggcatgac cggcggcttc gcttacgttc
    28501 tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgag gtcttaagcg
    28561 ttgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag catgttcagc
    28621 atactggctc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccactaaat
    28681 ttgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcat cgtagtcgta
    28741 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
    28801 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa gccgctgaag atccgcaaaa
    28861 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc
    28921 gctgcctgtc gtgcggcaac ccatactgcg agtggaaatg tccggtacac aactacatcc
    28981 cgaactggct gaagctcgcc aacgaggggc gtatttttga agcggcggaa ctgtcgcacc
    29041 agaccaacac cctgccggaa gtttgcggac gagtctgccc gcaagaccgt ctgtgcgaag
    29101 gttcctgcac tctgaacgat gagtttggcg cggtgaccat cggcaacatt gagcgttata
    29161 tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg aagcaaaccg
    29221 gtaaaaaagt ggcgattatc ggcgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga
    29281 cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc gggctgctga
    29341 ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct
    29401 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtgcagctgg
    29461 acgatctgct gagtgattac gatgccgtct tccttggcgt cgggacttat cagtcaatgc
    29521 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
    29581 ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg
    29641 aaggcaaacg cgtggtggtc cttggcggtg gcgacactgc gatggactgc gtgcgtacgt
    29701 ccgtgcgcca gggagcgaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
    29761 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
    29821 tccagccact ggggattgaa gtgaacggta acggtaaagt cagcggcgta aaaatggtac
    29881 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt
    29941 ccgaacatat cgttccggca gatgcagtga tcatggcgtt tggtttccgt ccacacaaca
    30001 tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgccccgg
    30061 aaggcagcga caacgccttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
    30121 tccgtggttc cgatctggtg gtaaccgcta ttgccgaagg tcgtaaggcg gcagacggta
    30181 ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga aaagagtcgg
    30241 cgctgaaatt ctcctttctt tctggaaagc gcctcgcata atctgaagaa aaccaatgta
    30301 atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc cattattcaa
    30361 cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc tcaacaggct
    30421 accagcaaat ccacgacggt attattcatc tggtcgatag cgcccggacg gaaacggtac
    30481 gtagcgttaa cgcgctgatg accgcgacat actgggaaat tggccgacga attgtcgaat
    30541 ttgaacaagg tggcgaggcc agggctgcgt atggtgcgca gctaatcaag cgactatcaa
    30601 aggatttaag tctaaggtat aagcgtgggt tctctgcaaa aaacttacgc caaatgaggc
    30661 ttttttacct cttttttcaa catgttgaaa ttcgccagac agtgtctggc gaattaacac
    30721 cattgggaat tccgcagaca ccatctgcag aattcccatc ggcaaagatt tggcagacgc
    30781 tgtctgccaa atctttcccc ctgccctggt ccacctacgt ccgtttactt tccgttaaaa
    30841 acgctgacgc ccgcagcttt tatgaaaaag agacgctccg ctgtggctgg tctgttcgcc
    30901 agctagaacg gcaaattgcg acccaatttt atgagcgaac actactgtca catgacaaat
    30961 cagccatgct gcaacaacac gctcctaccg agacgcatat tcttccgcaa caggcgatac
    31021 gcgatccctt tgtgctcgaa tttctggaat tgaaagatga atattcagaa tctgatttcg
    31081 aggaggcgct gatcaaccac ctgatggatt tcatgctgga acttggggat gattttgcct
    31141 ttgttggtcg gcagcgaagg ttacgcattg atgacaactg gtttcgtgtc gatctgctgt
    31201 ttttccaccg ccgtttacgc tgcctactaa tcgtcgatct aaaagtgggc aaattcagct
    31261 atagcgatgc cggacagatg aatatgtatc tcaactacgc caaagagcac tggacgctac
    31321 cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga gaaaggagcc ggagaagcac
    31381 attatgctct ggcaggtttg cctaacaccg ttctggcaag cgaatataag atgcaactac
    31441 ctgatgagaa acgactcgca gatgaactcg ttcgaacaca ggcggtgcta gaggaaggct
    31501 atagacgccg ttaatttcag cggtttcgaa ttctgcagac agtgtctgca gaatctttaa
    31561 aacgaactaa gttttaagcc attaaatcag ccttaacctt caccacaacc tttttaatct
    31621 ctccaggctc gccgacaacg catcctggtt tatgcggttc acctggcata aacacggcga
    31681 acattcccgg ttttaagatg atggcttgct cgttatcaat ggtgctgcaa agctgataat
    31741 catcctcatg gtggaactct tcacactggc gcgcagtgcc tgccatgcca aacagaatcc
    31801 gttcctcacc gtttaataac agttggatat caatgtattg ctcgtgcaat tccgcttttt
    31861 tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaagatattg tcgccctgta
    31921 attcgtaacg acccggcgct ttttcttgcg gtctggcagc taatgccagc gttaacgcgt
    31981 cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc atcatcataa
    32041 tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcgtggc ggtagtgcgc
    32101 cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt ccaccagcgc
    32161 cagataccct tctgccagcc caacgctgcc accgaccacc acgcactggc aatcagttgt
    32221 ggctttaata tcagcgatca gccttgcaag cgtacgtgcg gagcggtgaa tcagctgctg
    32281 cgcctgctcg tcgccctgcc cggcgcgtgt gaaaatagtt ctcgcatccg cgcccgccag
    32341 ttcaccctgc gctgccgccg caatgccgcg accagaagca atcgcctcca cgcaacctgt
    32401 gcgtccacag ccacagaccg ggccgtgtgg atcggcaagc gtatgcccga tatgccccgc
    32461 cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc cgacgccagt
    32521 ggaaacggtg ataaacacca tctcggttac atcgccttcc agcgcctgat actccgccca
    32581 tgctgcagcc tgcgcgtcgt taatggcaat ggtcggcaaa ttggtaagtt gttccagcgt
    32641 tttgactaac ggaaagtgta gcaatccacc gagattatgc ggattcagcg ccagcaaact
    32701 gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag cttgcaacgg
    32761 agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg ctggcgtagg
    32821 aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg caagtttagt
    32881 accgccgata tcaatcgcca gcgtggtcat agcaccgcct ttttcatcgc tgtgttgtac
    32941 cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc ccacgcgccg
    33001 tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac gcccttcggc aatcacccta
    33061 catccggcgt cgctcaacgt tttcaccagc gccagatccg gctcttctgg cgtttcaggc
    33121 gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg gcatgccagg
    33181 ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatggtg aatacgtgcc
    33241 agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc gataatgtcc
    33301 gcgcccgcct gcgccagcgc atcaacatct tcaatatagg ccgtgatgcg taccggagaa
    33361 tcctccagat cgcgtttcac aattccaata atcggcacgc tcaccaccgc acgcgtggct
    33421 tgcagatttg ccacaccttc aatgcgaatg gcaaccgcgc ccgcctgttc tgccgctaat
    33481 gccatggcgg cgacgatttc gggtttatcg agcgggctgt ccggaaccgg ctggcaggag
    33541 acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa cgacatacat
    33601 cttcccttag cgaaaggccc ggtacataga ccgggcaaca ggattaactt ttggttttga
    33661 ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct aaatggtttg ccgtcgatag
    33721 cgtcatgagt acgcaatgct tccgggcgca gccaacgctg aacgcgggaa ggcatatcca
    33781 gaccaatcag caggatcacc acaaatgtca gactgaacga tagcgatgcc agcgcagtac
    33841 ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg ccgcccaatg
    33901 cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg gtcggtatcg aaataaccgc
    33961 caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg aagaacagta
    34021 acagaccgag cacccagacg tttgcgccgc caatcgcaaa taccggaata atcaacagct
    34081 gcgaggccaa caggctacaa acgtaggctt tgcgggttcc cagccagtca ccgaggaagc
    34141 cgccgacaca gcaccccacc gcagccccaa agccgctaaa gaacagcaca ttggcgacag
    34201 tatgcgggtc ataagccaga tcggttttca gatacgttgg cagcagcgcc tgaatcggcc
    34261 atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgcccgtt ggccagcgtt
    34321 tgcccgtact ctgcaccata aagctgataa agattgcggc gcataacagc ccaagaacag
    34381 cgacgatcgc ggcattttgc aggttaccgg cgaagcagaa ccacagcgca gtagccgccg
    34441 ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata tccaccattg
    34501 tgcgtaccgg tgctttaccc ccgtgtttct ctttccagtc ttccgcttcc gggatgtttt
    34561 tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc gcacgccagc
    34621 cccagaccgg aaccaccagg ctatagacct gagcggcaac gacggccccc acagagaagc
    34681 ctgaaatcag aaaaccactg gctttgttac gcagatgttt tggccagctt tcaatgacat
    34741 aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga cgcgcgataa
    34801 acatggtgat gtagcctggc gcaaagccgc aggccagcgt cccggccgag aagagaacga
    34861 tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg agcatcaggc
    34921 cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc accgtcgtca
    34981 gcccgaattc accctgtact tcggtgagta ccagggcgat taaaacgaaa tcaaaaccgt
    35041 caagcagata tcccaaccag gcagcggaaa atgcgcgcca ttgtgcacgg ttgagatggc
    35101 gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg tgggcgatgc
    35161 ccacacgctt tggtataaaa attgtagggt acagatgcgt ttatttcccc tcacccggta
    35221 ggggcgagcg aggggaaaca actcacccgc gctcttgcat caactgctgg gccagcgcct
    35281 tcagttctgg cagatatttt tcatctaccg gtccaaacgg tttgcggcac agcggcacag
    35341 aaacgacatc catataatgg aggacagttt tcaggccgcg gaatacgccc gttttgatca
    35401 gtaaatcaat gactttattg cattcagttt gcagtttctg cgcggtctgg atatcgcctt
    35461 ctttcagcgc cttaacgatc ccctgatagc gccagcccat gatgttgtag gtactaccga
    35521 taccaccatc agcgcccgcc agcagaccag aggcgaagat ttcgtcgtaa ccgttataga
    35581 gcacaagatc aggatgttca cgacggatct gctccatctg atagagatcg ccagaggtct
    35641 gtttcagcgc acctacgcca ggcaatgtaa caagtgtgtt gatctgatcc agggtcagtt
    35701 ttaccccact cagggctgga atgttgtaca ccaccatcgg caaaccatcc gccgaatcaa
    35761 taattgcccg atagtgatcg cagtgttctt caaagctgaa aggatagtag aacggcgtga
    35821 cggcggagac ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt tggctttcgg
    35881 cggtgctgac gcaaccgacg tgggcgatga gtttaatctt acctttcgcc tcttcggcga
    35941 cgatttccag tacctgttca cgctcggaaa ggctttgtac aaaggcctcg ccggtcgaac
    36001 cacccacgta taaaccgtcg atgccctgct gaatattgaa ctgaaccagg cgacgcagac
    36061 tcgctttatc cagtgcttgt tgttgatcaa aaggagtcag gagtgcagcc attacgccac
    36121 gtaaattcgt tgccataaat acctctgaag tgatgcttgt ctgataaacg atataccttt
    36181 atacctgtta taccagatca attaagcaac accccataca gaaagcttat aatgcgatct
    36241 gcttcactaa agtggcatta tttctttttg ttggtggtct gaccgaaagc gtgccaggta
    36301 gcagagacgc tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg atcatgacgg
    36361 cggatcgcat caacgatcgc aatatgctgt tgataactaa cgttgttatg ttcgtgcagt
    36421 gcctgatcgg taaccgttgg gcgtgcggca ataagccagt cgagcagggc aacgtggatc
    36481 gccatgaaga ttgggttacc ggggatctcc gccagcacgc ggtggaaatc aacgtctgaa
    36541 cgaatgaatg ccgcgttgtt atccagcgac tgactgttaa tttccagtgc ttttgccagc
    36601 aaatcgattt gctcatcggt ggcatgttca gccgcatagc gcaccaggct ggattcaaag
    36661 aacagacgta attgttcgaa atgggcaatc ccaccaggat gagaaaggaa atctttcgcc
    36721 atgccggaaa gctcgccgat gatggtgtcc gcagaaggac gtgagacgcg ggcacgttcg
    36781 ccgttgttga tttgcaccag acctttgcgt tttaacgctg ccagcgcttc gcgcacagaa
    36841 ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag acggtaattg ttcaccttcg
    36901 ccaaattcac gacggcggat catctgttcc agctcttctt ccaccatttc ggagagtttt
    36961 ttacgcgcca gcgggcggct acgcaagttg cgaccaattg caggtgaaga atcttcggtt
    37021 tgcgaatcaa atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta acatcatcag
    37081 tgacatccta tcacaggatt gaaagtaggg gaaaatggca ggtttttctc tttgtgcctc
    37141 atcattacca taattaacgg aataattaac tattgcgaaa aattaatgta acgcagataa
    37201 aaacatcccg tttgaattat ttataagact attcacgagc attatgaata ttatgaatgt
    37261 gttcttacaa aataatcata agcgcatatt ttttaatgaa aaatcacctc acctacaatt
    37321 aaaaacacga catccgtacc ataaatagac ttgcaaaaaa tataacatca ttgttttcaa
    37381 tctgccgttt atgggattga ccgttttctt ttgacacgga gttcaacaat gttcggcata
    37441 attatatctg tcatcgtatt aattacgatg ggctatttga tcctgaaaaa ctacaaacct
    37501 caggtggtgc tggctgccgc aggtatcttc ctgatgatgt gcggtgtctg gttagggttc
    37561 ggtggtgtac tcgatcccgc caaaagcagc ggctacttga tcgtcgatat ttataatgaa
    37621 atcctgcgca tgctgtccaa ccgcattgcc ggattggggc tatcgattat ggcggtgggc
    37681 ggttatgccc gctacatgga gcgcataggg gccagtcgcg cgatggtgag cttgttaagc
    37741 cgcccgttaa aactcattcg ctcgccgtat attattctgt cggcaactta cgtcatcggt
    37801 caaatcatgg cgcagtttat taccagcgcc tccggtctgg gtatgttgct gatggtcacc
    37861 ttatttccga cactggtgag tctgggagta agtcgtctct ctgcggtggc ggttatcgca
    37921 accacaatgt ccattgagtg gggaattctg gaaacgaact ccatttttgc agcccaggtc
    37981 gcgggaatga aaattgccac ttacttcttc cactaccagc ttccggtcgc ctcttgcgtc
    38041 attatctcgg tggcgatctc ccactttttc gtgcaacgcg cttttgacaa aaaagataaa
    38101 aatatcaatc acgaacaagc agagcaaaaa gctctcgata atgtcccgcc gctctattac
    38161 gccattttac ctgtgatgcc gttaatcctg atgctcggct cgctgttcct cgcccacgtc
    38221 gggctgatgc agtcagaact gcatctggtg gtggtgatgt tactgagttt gacggtgacg
    38281 atgtttgttg agttcttccg caagcataac ttgcgcgaaa caatggacga tgtgcaggcg
    38341 ttttttgacg gcatgggtac gcagtttgcc aacgtggtaa cgctggtggt cgcgggtgaa
    38401 atatttgcga aaggcttaac gacgattggc actgttgatg cggttatcag gggtgcggag
    38461 cattctggtc tgggcggtat tggcgtgatg attattatgg cgctagtcat tgccatttgt
    38521 gccattgtga tgggctctgg caatgcgccg tttatgtcat ttgccagtct tattccgaat
    38581 atcgcagccg gactacatgt accagcggtt gtaatgatta tgccgatgca ttttgccacg
    38641 acgctagcgc gcgcggtttc gccgattact gcggtggtgg tcgttacgtc aggaattgca
    38701 ggcgtttcgc cttttgcggt ggtgaagcga acagcgatcc ccatggcagt cggtttcgtg
    38761 gtgaatatga ttgccacaat cacgctattt tattaagcca ttaaaagaca aaacaggcca
    38821 ccagggcctg tttttgtatt acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg
    38881 ctgcggaggt tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat
    38941 aaccgacata acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc
    39001 ttcatcgtag gcagcttcag gctcaaacat tgtgcctgcg ccattttcac gggcgtagat
    39061 agccagcacg gcagccagcg gcacagaaac ctgacgcgga atgccgccaa agcgcgcgtt
    39121 aaagcgcacc tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag
    39181 tacgatttgc ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc
    39241 caccaccagg tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag
    39301 atagggacga cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc
    39361 atttcacgtt ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc
    39421 atatagcctt tcagctcttt cgcacccggg ccgctgaact cgatgcccag ttgcggcaga
    39481 cgccacagca gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac
    39541 ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg
    39601 gcatctgctt cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt
    39661 tcgatacgat gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt
    39721 ggcggatgcg ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc
    39781 agctcacgat ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga
    39841 ggcggattgt ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca
    39901 atgcggacct gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt
    39961 ttgttggcag cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag
    40021 ccaccaggtg gccagtcaga agttgtgtta cccagtaagg aacgactctc tttgttcgaa
    40081 aatcaaacaa aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc
    40141 agattttgcg acctttgtgg tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta
    40201 gtctgaaata gttgacttag tcccttattg gcgatgtggt ttttgttttg cctgtctgtc
    40261 agacggtagc aaaaagcaac tttccaattt ttacgctgat tcagaattta accataaaaa
    40321 aacccgccga agcgggtttt ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg
    40381 tttggagaac tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg
    40441 acgagcgtca cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta
    40501 ttccatcaga gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc
    40561 acctttaacg gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg
    40621 acgaactacc atgcgagcag tttcacgacc gaagtactgt tccagagaac gttggttgat
    40681 tacgatttta ccgttgcccg gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc
    40741 agtgccgtag tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg
    40801 cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat
    40861 agcacgaccc agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc
    40921 aggacggcga gcaatcatct cttcaaaggt cgcttgtttg ataccaccga tgtggccggt
    40981 gtggtgatag tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt
    41041 cagaacgatg atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc
    41101 gcgcaggcga cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac
    41161 aacataccag tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt
    41221 acccaataaa tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca
    41281 cgacaaagtc cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg
    41341 ggaaaaaaac tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat
    41401 cgaccccttt ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac
    41461 tcttcgcttt gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga
    41521 tcgccctgat aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc
    41581 tcgtaaaact catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc
    41641 ataactggta catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca
    41701 tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg
    41761 acgcccattg tcgccaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc
    41821 gcccccgcca gcgccaacca tagtttatcc atctgcgccc gggtttcatc gttgagtggc
    41881 gaaagccaca gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg
    41941 ttcattacat cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc
    42001 aggccatttc gataaagttc atccggcgga atatttgacg tcgctaccag ggtaatgccg
    42061 cgggcgaaca gggctttcat cagaccgcca agtagcatgg cgtcggtaat atcagaaaca
    42121 aaaaattcgt caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc
    42181 agcggatcgg tctgcccctg taaggcagtt agctcttcat gcacccgcat cataaaacgg
    42241 tgaaagtgca ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc
    42301 caggttttcc cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt
    42361 gtgtcttcgc gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg
    42421 gctggtggcg tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct
    42481 ttttgaacgt cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt
    42541 ggggtaacgc tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca
    42601 cggttgacga aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg
    42661 tggaattaac ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc
    42721 tacggaaaca aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta
    42781 gtcgtcggca tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag
    42841 caacaggcgt tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa
    42901 gagctggtta gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat
    42961 cgccagctgt atcagcacat ggcgaaaagc tccagcagcc tgctgccgga actgtctgct
    43021 gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg
    43081 gtgcagatgc ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag
    43141 cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac
    43201 tatcatttaa tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt
    43261 ttgaatctct tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaacccag
    43321 ctgttgagtg cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc
    43381 gcgtcgattc caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg
    43441 gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag
    43501 aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt taccggatca acctgcacaa
    43561 cccttcgaag gtttaggttc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc
    43621 aacaaccatg tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag
    43681 tttgatgcaa aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa
    43741 aacccgagca aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat
    43801 tttgccgtag cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt
    43861 tccgcattag gacgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat
    43921 gcttccatta accgcggtaa ctccggcggt gcactgttaa accttaacgg tgagttaatt
    43981 ggcatcaaca ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc
    44041 cccagtaata tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc
    44101 ggtttgttag gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt
    44161 gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc agcgaaagcg
    44221 ggcgtcaaag cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct
    44281 gagttgcgct ctcgtatcgc gaccaccgag ccgggcacga aagtgaagct tggcctgctg
    44341 cgtaacggca agccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc
    44401 agcgctgaaa tgatcacgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa
    44461 gatggcggca aaggtattaa gatcgatgaa gttgtcaaag gaagcccagc tgctcaggct
    44521 ggcttgcaaa aagactatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct
    44581 gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggc
    44641 aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt
    44701 gatgtccggt taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca
    44761 tgtttgtgaa gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg
    44821 ttgccatgcc ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg
    44881 atgagacgcc tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg
    44941 tttacaaccg tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat
    45001 ccggtgtaat catggatcaa cgcggttata tcatcaccaa taaacacgtc atcaacgacg
    45061 ccgatcagat catcgtcgcc ttacaggatg gtcgtgtatt tgaagcattg ctggtgggat
    45121 ctgactctct aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca
    45181 ttccgatcaa tgcacgtcgc gtaccgcaca ttggcgacgt agtactggcg atcggtaacc
    45241 cgtacaacct cgggcagacc attacccagg ggattattag tgccacgggt cgaatcggtc
    45301 tgaacccgac cgggcggcaa aacttcctac aaaccgatgc ttccattaac cacggtaact
    45361 ctggcggcgc gctggtgaac tcgctgggcg aactgatggg cattaacacg ctgtcgtttg
    45421 ataagagtaa cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag
    45481 caaccaaaat tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggta
    45541 tcggcggacg cgagatcgca ccactgcacg cgcagggcgg tggtatagat caactgcaag
    45601 ggatcgtggt taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca
    45661 acgatctgat tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc
    45721 aggtggcgga aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc
    45781 agttaacgct gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa
    45841 aacaaaaacc ggagtctgtg ctccggtttt ttattattcg ctaatcaatt acttattaac
    45901 gaactcttcg cccagggcga tatctttctt gagcgtatcc agcatacctt ccagcgcgtt
    45961 ctgttcaaat gcgctcaggg taccgataga tttacgctct tccacgccgt ttttacccag
    46021 cagcagcggt tgagagaaga aacgggcgta ctgaccgtcg ccttcaacgt aggcacattc
    46081 gacaacgcct tgttcgccct gcagtgcacg aaccagagac agaccaaaac gtgcagctgc
    46141 ctggcccata gacagggttg cagacccgcc accggctttc gcttcaacca cctcagtacc
    46201 cgcgttctgg atacgtttgg tcagatcagc cacttcctgc tcggtaaaac taacgccagg
    46261 aacctgtgac agcagcggca gaatggtaac accagagtga ccgccaataa ccggcacttc
    46321 aacttcgcct ggctgtttgc ctttcagttc cgcaacaaaa gtgttggaac gaatgatatc
    46381 cagcgtggta acgccgaaca gtttgttttt gtcataaaca ccggcttttt tcagcacttc
    46441 agcagcaatc gcaactgtgg tgttaaccgg gttagtgata ataccaatgc acgctttcgg
    46501 gcaggttttc gcaacttgct gtaccaggtt tttcacgatg ccggcgttaa cgttaaacag
    46561 gtcggaacga tccatacccg gtttacgcgc tacacctgca gagataagaa cgacatctgc
    46621 gccttccagc gccggagtcg catcttcacc agaaaaacct ttgattttca cagcagtagg
    46681 gatatggctc agatcgacgg ccacaccggg agtcactgga gcgatatcat acagagagag
    46741 ttctgaacct gaaggcagtt gggtttttaa cagtagtgca agcgcctggc caataccgcc
    46801 agcagcgccg aggactgcga ctttcatcct aaactcctta ttatattgat aaactaagat
    46861 atgttgctcc gctgccgcga ccttaatcca caaaattgcc gtttacaatg accacatctc
    46921 aagaatgtgt agtcacgcaa gtttagcgtt tatgcattta attgccgtaa tcaggaacct
    46981 aacttacgta attaacagtc gcttatgcat tagcgcaaca ttccaacagg tggtgacaat
    47041 ataccctacc gttcagccaa aacaacatca atttgataac aattaattta cttttaagca
    47101 gaatttgcat gccgtgacgc aggcatgttt ctcaataacg aaatttgata aaatcccgct
    47161 ctttcataac attatttcag ccttcttcag ggctgactgt ttgcataaaa attcatctgt
    47221 atgcacaata atgttgtatc aaccaccata tcgggtgact tatgcgaagc tcggctaagc
    47281 aagaagaact agttaaagca tttaaagcat tacttaaaga agagaaattt agctcccagg
    47341 gcgaaatcgt cgccgcgttg caggagcaag gctttgacaa tattaatcag tctaaagtct
    47401 cgcggatgtt gaccaagttt ggtgctgtac gtacacgcaa tgccaaaatg gaaatggttt
    47461 actgcctgcc agctgaactg ggtgtaccaa ccacctccag tccattgaaa aatctggtgc
    47521 tggatatcga ctacaacgat gcagttgtcg tgattcatac cagccctggc gcggcgcagt
    47581 taattgctcg cctgctggac tcactgggca aagcagaagg tattctgggc accatcgctg
    47641 gcgatgacac catctttacc acccctgcta acggtttcac cgtcaaagac ctgtacgaag
    47701 cgattttaga gctgttcgac caggagcttt aatctctgcc ccgtcgcttc tggcggcggg
    47761 gaaaatgttg cttatccctc tcaaccccct gctttctcct gcgatacatt taacgaatag
    47821 tgcgttttac tgcgacatgt cattcacaca atgaatacat aaggtaaaaa aagcacatta
    47881 tgcaaaattc attatctaat tgaaaacact agaattaacg ataaataacc gtatttttaa
    47941 ttcttttttg ttattaacat tcacattttt aacacttagt atcaacagaa acagttagcg
    48001 tggtattaat tagttcaata attagtgtat acttgatttt gtgataaggg tcacgaaaca
    48061 aagacccagc aaaaagatta tgtcgaggta aaaatcatga aaatcaaaac cactgttgct
    48121 gcattaagcg tactttctgt tctctctttc ggtgcattcg ctgccgactc cattgatgct
    48181 gcacaagcac aaaaccgtga agcaatcggg accgtatccg taagtggtgt ggcgtcttcg
    48241 ccaatggata tacgtgaaat gctgaacaaa aaagcggaag agaaaggcgc aacggcctac
    48301 cagattactg aagctcgtag cggtgacacc tggcacgcta cggctgaact gtacaaataa
    48361 accctcatcg tcttgtccga cgatattgcc cccggttcgg gggctttttt ttgcgctaat
    48421 gacgaacatt aaaacgcaaa tgcccttcca gctcttcctc tgcctcatca aacagcaata
    48481 ttaacgcgcc aaaacggcga cgcgttttct ctcccagatg aacaaattca atctcaagtg
    48541 gtagcggcag gacatcgttc attaacacat cccatagtga gtcgagatcg cgtaccttat
    48601 ctttcgccag accaaaggct tggctgaagt cgcgataaaa atcctcctga ctctcaatct
    48661 catcaaaatc aaaggtataa atattcatct gttgccaccg tcacgtttcg cggcgagttc
    48721 tgccgttcat cgctaagtat agccatgaaa aaaccgacgc ttttggcgtc ggttttgact
    48781 taactatcgg tcaacgcatg ttgatactta tggagcatcc ccgccagccg atgaaccggt
    48841 tccgtcacct gcggtggcgc ttgccagatg cgtagctttt cctggtagat ttccagttct
    48901 tccagcaact gaccaaagta ccgacgacgt ttatcatcgc tacgggcaga tatcacatgg
    48961 tctgctgtgc ggcgcatttg tcggtgaaac gccgataaat cctcgttaac cgggatcggc
    49021 gcatcacgca ggcgctggtg cgcgataatc atcgtcagcg ccaggcgaaa tttcggcaaa
    49081 tcccctggga acttattcat cagcaaaaac agctgctgat aaagtgccgg gaggtggttc
    49141 tctttacgac gtgccacatt ggtagtcatc gcggaaacag cggcagaaac aaactgatta
    49201 agcagtacac gtccagtcct gtcgcgcgat ttatcccgca ccagcaaaat aacggtgaac
    49261 gcgagcacac agccgacgat ttgccctaat gcgctgtcga gaaactgact gaaatggaaa
    49321 gtcatcgggt tatccagcac gataatattt atggtgctgg ccagcgcccc catcgagccc
    49381 agtcgccgtt tctgtacttc tattccgagg aagaatccca gcactgccag gctaatacac
    49441 agcagcaaca tgctctgttg ggtattaggg ataatcacca aaaagtagag cagccctaac
    49501 ggcagcgcgg ccagcgtccc gtagataaag tcgatcgcca ccatgcgtgg attcggcaaa
    49561 cgcattgcca gtgacgtcac taccgcaatc atcaccattg caccactgcc ggaagtccag
    49621 cccgtccaca gccagaaaag cgtgcccaga atgcaggaaa gtgtggttcg ccagaagtta
    49681 accatcgcat gatgacgttc ggctgactct actttgactt ccggttcgcc ttgcaggatc
    49741 tcttcttcgg tggcgttgat ttttgtgtta ctgataacgc cgcgcttgag aagctgataa
    49801 cgcgttgccg ccgcgaccca gctataaatg gtgacaggcg tttcccgttc cccggtccag
    49861 gcgataactc tccgcaggcg tttgagctgc ttgtggacgt cctgcgcggt ttctaccggc
    49921 gtgtcaaaaa attcgcggaa agtatcagtg atcaattccg ggcgcgtatt ctgaataaga
    49981 taagtttcgc aggattgggt aatcagcgtc agcgatagcg tattgatcgc ttttaaacgt
    50041 cgattggccc gcgcccagcg ggaagattcc atattcaggt tgctgcgcat gccttgtagc
    50101 gccgtggtgc gtcgcaccag gtcgccccag gctttatcga caacttcacc atcgccatgc
    50161 ttgatacaga gttgcattaa ttgatattgc gcgaccagca aactttccag ctctcgatcc
    50221 acttcttgtt tgatcgatcg cggagaaaag agcaaatccg ccataatcgc acacacaata
    50281 ccgataacga tctcgctaca acgttcgacg gcaaactgcg gcgtaagcaa tggttccggc
    50341 tgaatggtga tcacaatgat cagcgcggta taaccggcca gcccccacgc gtacgagttt
    50401 tctattcgta ccagcgagga tatccaggta caaaaaccgg cccagataca gcacaccaga
    50461 atcatcaata atggtgcgcg gatcatcgca atgatgatca ccagtccggc aatacagcca
    50521 ataaatgtgc cgatgatgcg caaaaagcca cgataacgaa tagcgccaga atacggttca
    50581 cctcccgcag caaaggccgg accggcggca acaatcgccg ctgtcagtac cgcccagcgt
    50641 ggcgtttcca gctggaagtg aaagccaaca aacagcgcca gtacaatggc ggtcgccagt
    50701 tttaccgcaa agcgaatatg ttggttagca atggagaaaa tacccatcgt gattaaccaa
    50761 actcacgcag gcgatgggcc attttacgga agaacgaatc ctggctttcg tcgcgatctt
    50821 gtttgccagt gaccaccact gtagcagtgg tgcccgcagg ccagatgttc tcttgctggt
    50881 tgtcgagacg aatacgaacc ggaacacgtt gcgcaagacg cacccattca aggttagagt
    50941 ctatagtcgc catccctttg tcgtcacgcg tgctgctggc gttggtgacc cctgcggcaa
    51001 cactatcaac agtccctttc agcactttgt tactgccaag cggcgtgatc tctgcacgat
    51061 accccggacg caccccttcc agcttagttt cttccatata ggccagtaca tagaaggagt
    51121 tctgtttcac cagcgcaacc gccgttgatc ctcgagtaat aaactcaccg gtatagacgt
    51181 tgaggttggt cacccagcca tctgctggcg cgcggatcac cgtgcgttca agatccagtt
    51241 ttgccagatc gcgggtcgcc tgcgctttcg ctaactgatg cagaaccgtt tgtagtacgt
    51301 tgttagcctg gtcgatctct tcgcgagaca tcgcctgcac accgagacgg ttacgacgtc
    51361 cggcctcctg gcgtttctcc tgtgccagta cctgataata agcaacatcg gcttgcgctt
    51421 cctcaagcgc cttttgatag cgcggctggt cgatggtgaa cagtatctgt ccttttttca
    51481 ccagctggtt atcatgaaca ttcacctggg taatgagtcc agaaacgtcc ggcgcgatcg
    51541 caacgacgtc agcgctaaag cgcgcgtcac gcgtccaggg ggattcggtg taatagaccc
    51601 aggcattaaa aattgcgatg aaggccagaa tgactaatac gaccgtgatg gccgtacggg
    51661 agaattttct tattagtgtt ttcacttcaa cctcaaacga acagtcgcga tatcaaataa
    51721 aacaagcagc aatagagcgc ggtgttgaac aacgccggat gccagacaaa gtcgtagata
    51781 cctgttggca caagtacccg gcgcaccagc cagaaaatcg ccagtgataa aagcaattca
    51841 aaaaatatcg gtgggaagga cagcccaaac accacgataa cgggaaacag actcatgttg
    51901 accttggttg taaagagaga gcaggcgtta ttattttcag catctgtcgc cgcagagaag
    51961 ggcatggaaa gccgggcgag agcaacattg ctgtagattg atatttaata tattagcgta
    52021 actgttatgc tgttatctat attatgtgat ctaaatcact tttaagtcag agtgaataat
    52081 ggaacgacta aaacgcatgt cggtgtttgc caaagtagtt gaatttggct cttttaccgc
    52141 cgccgccaga cagctacaga tgagcgtttc gtccatcagt cagacggtgt caaaactgga
    52201 agatgagttg caggtaaagc tgttaaaccg tagcacacgc agcattggcc tgaccgaagc
    52261 cggtagaatt tactaccagg gctgccgtcg tatgcttcat gaagtgcagg atgttcatga
    52321 gcaactgtat gccttcaata acacccccat cgggacgctg cgcattggct gttcttcaac
    52381 tatggcacaa aatgttctcg ccgggctgac agcaaaaatg ctgaaagaat acccaggttt
    52441 gagcgtcaat ctggttaccg gaattcctgc ccccgacctg attgccgacg gtctggatgt
    52501 ggtgatccgc gtcggcgcgt tgcaggattc cagcctgttt tcccgccgtc tgggcgcgat
    52561 gccaatggtg gtgtgcgccg caaaaagcta tctcacacaa tacggcatac cggaaaaacc
    52621 cgccgattta agtagccatt catggcttga atacagcgtg cggcccgaca atgaatttga
    52681 actgatcgca ccggaaggga tctcgactcg cctgatcccg caggggagat ttgtgactaa
    52741 tgatccgatg acgctggtgc gctggctgac tgcaggcgct gggatcgcct acgtgccgct
    52801 gatgtgggtg atcaacgaga tcaatcgcgg ggagctggag atcctgctac cacgttacca
    52861 gtcagatcca cgcccggttt atgcgttata taccgaaaaa gataagctgc cgctgaaggt
    52921 acaggtcgtg atcaactcgc tgacggatta ttttgttgag gtcggtaaat tgtttcagga
    52981 gatgcacggg cgcgggaaag agaagtaatt catattgtac tgttacgttc tacaaacctg
    53041 tgccaacggg ttcccctcac cctaacccca gaggagcgag gggaccgttc gtgcacgtag
    53101 aaagattaat tatccttctg aaaataatga aattattacg cagtaccgcc aacagtcagg
    53161 ttatcaactt tcagcgttgg ctggcccacg ccaaccggca aactttgccc ttctttaccg
    53221 cagacaccca cgccgttatc cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg
    53281 gtttcgatac cggaaccaat caacgtcgcg cctttcaccg gcttcgttac tttaccgttt
    53341 tcaatcagat acgcttctga agtggagaaa acgaatttgc cggatgtaat atccacctga
    53401 ccgccaccaa agttcggtgc atagataccg tactcaacgg attcaataat ttcctgcggg
    53461 gtcgatttac ccggcagcat ataggtgttg gtcatacgcg gcatgggcag atgggcgtag
    53521 gattcacggc gaccgttgcc agtcggcgtc atccccatca aacgcgcgtt gagtttatcc
    53581 tgcatgtagc ctttcagaat gccgttctca atcagcacgt tgtactggcc tggcgtacct
    53641 tcgtcatcaa tcgccaccga accacggcga tcgaccatcg tgccgtcatc aaccacggta
    53701 cacagttctg aagccaccag ctccccgacc tgcccactaa atactgaagt gccgcggcgg
    53761 ttgaagtcgc cttccagacc gtgacctacc gcttcatgca acagcacgcc cggccaacct
    53821 gcgccaagta ctaccggcat ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc
    53881 gccatacgca ctgcttcttt tgcccatgca tccgcacgga cttcgccgtc aagatcggca
    53941 aggaagaact cataaccaaa acgaccgccg ccgccactgg caccgcgttc gcgtttgcca
    54001 tcttcttcga ccagaacgct cacggaaaga cgcaccagcg gacgaacatc cgccgccagc
    54061 gtgccgtcgg tggccgcaac cagaattaat tcataaacgc cgctgaggct ggcagtcact
    54121 tcttgtacgc gcttgtccgc ttcgcgggca accttatcga cgcgacgcag gatatccagc
    54181 ttctcttcac ggctcatgct ttgcagcgga tctaccgagg tatacaacgg gctatgctct
    54241 accgcgccca gcgtctgtac tttgccatca ccactatcac ggacgatggt gcgcgccgct
    54301 tgcgcactct gttccagcgc cagcaggctg atttggtcag cgtaagcaaa tccggttttt
    54361 tcaccactga ttgcacgcac accaacgccc tgatcgatgt tgtaagaacc atctttaata
    54421 atgcggtctt ctaaaaccca ggattcgtga tagctcgact gaaaatagag atcgccataa
    54481 tcaaggcgac gttcggccag ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg
    54541 ttcgccgcta gcaattgttc acttaccagg ttaagactca tcgtttttgc tactcgttag
    54601 ttactgcagt agaggatttt ttacggctgc cggatgcggc gtgaacgccc tatccgacct
    54661 acggttatgt tctggctcgt tggcctttgg caacgattat catatgagat tggggcaatt
    54721 acgcgccctc gtcaaatcat tgcgcttttt ctttacgcgg ttggcgcaac acttcgttga
    54781 tttgcggatc gtccagcgga cccgaaatgt gatagcgcaa aatggagact ttgctccaca
    54841 gcggccccag cactttactg gcggcaaaca ctgccgcgcc aacaatgggg ttaaccgcaa
    54901 aagccgcagc cacgcccacc gtcgcagaaa tctctggtgc gacaaccgct tccatattca
    54961 ggtcgcgacg taccagattt accgaccctt tcatggcgat atccgcctcc aggccatcca
    55021 ccagcgtgtc gtcggtgtgc ataacgccgt ctttaatcca cgcggtgctg cgaatggagt
    55081 caaaatagaa cccttcgcca aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg
    55141 catctacgct caataagcgc agcaattgcc cggcatgtcc ggtattgatt tcggtaattt
    55201 cgcctttacc cagttgagta tgaatgatgc cattcaacgt cgcttcatct ggctgccagg
    55261 gtgctttgcg ccagtgtaaa tcgtaatcca cattaaatga cgactggcgt atgggcgtcg
    55321 tgacaccaaa aaattctgcg gcggcatcaa ttttctgccc gcgcagtttt cctttcagcg
    55381 aggtacgttc attccccgga ttattaaccc attcaccatc ggcagtaagc cgcgagaaac
    55441 cagtatcaat cagtccattg gtcagcgtta acgtatcgcc agaaatggtg atatcactgt
    55501 caatgcgacc gaatttttgc ccccagaacc agcactctgt gcatcgtatt tgggcgtccg
    55561 gccagccacg gaagttaatg cgctccgttg tcgggaaagg tgatgacggc gttgaatcac
    55621 cacgagtttt cgccacgctc gggttgtaat aaagatattt gatattcgcc agccacggcg
    55681 cgttattacg catcgctagc gtggcgttga tttcacgccc ttgcgcctca accagggtgc
    55741 catttgccgt cggttgcgaa acaatactca ggttattcca ttgctgattt cccagtgaca
    55801 acataggcgt acgtaacgtt atgtgttgtg ggaaactcgc tgcaccaccg acactctccg
    55861 cagcgccttt ctggaacagg gccagccact cggcaccatt catcggcggc atattgagtt
    55921 caacgccact ttgttccggc aacggcggga gcgttttact gtctgccgcc caaatagcac
    55981 gatcgagcgt cagcttttga ccaagcaacc agcggctatt gaaatggtta tccgcaccag
    56041 cctgtccggt taattcaaag ctgttgagat tgccatcaac cttaacgttt accgctagtg
    56101 gttcacccgc aggtttggct aacggtgaag gtaagtgact actcacattc ttcagatcgc
    56161 cgttcagctc tacgttatag gtcgcgccag catgataagg cagatcaatg cccactttac
    56221 catcccacgc cacgctgcca cttaatgctt cgttcaccgc ttcaggcaaa acgccggttt
    56281 tcgccggttg ccagttaccg ttgagattca ccgcaacctg gtaggctttt gccccttctt
    56341 tggtggaaaa atccacgttc aacggctgat taaaccagct tgctgtcagt ggttcacttt
    56401 gcagatcgcc attgataaag ctgaatttac cgctcaaatt tttcagggtg ctgtcgagtg
    56461 gtttgataaa cagactgtta ttacgcagcg tcacttcacc tttcgcggtt accagctcgc
    56521 cgttcagcgg gatatcaaga tgtaagcgag cattcacatc gccgtcgagc tggagttctt
    56581 gcagggtcgc acccagggaa tctttcagcg gtgtctcatc aaagtaaggg cctacggctt
    56641 tacccggacc tttaatgtca gcgtcaatca gcagtttttc ttttgagtag tcagggatca
    56701 ctgcggtgag attactcgcg cgcacgccgc ccagattaac gccatcggtt ttcatccata
    56761 aaccgtcgtt aataaagtcc agttcaatat caaggttagt taatgcaggc cagtccggct
    56821 ggaaggcaaa cttcgcgttg cgcagcggca ccagcacttc aaactgacct tcgttgtgtt
    56881 tataggggaa gagttgcgga ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc
    56941 cctgaatcgc accacttaaa taatcaacca ggtctttacc catcaagttt tccgggaaat
    57001 agcgccaggc ttgtgaacca tcatcggtac tgatgccagc cagaataccc agccagggtt
    57061 catcgttagc aggttgcagg taacgaaaac cgccgcgcgc atggacggct ttggctttaa
    57121 cgtcaatatt acgcccatcc agctggaaac ctttgtcatt gttcagccag cttatagttg
    57181 cctggccgtc ggcgatttcc agtggcgcac ggaataccgt ttcgtaaggc atctttgcct
    57241 gcttcatcga cgcggtaagc aaaccatttt caacgctgcc ggaaagcgtc ccggagaagt
    57301 gttccgcacc cggtaataat ttccattgct tccaggccag atcgctccac gatgcctgaa
    57361 aacgggtctt gtctgccgcc tgaagcggga tatccagcgc cagagtgtta atcttgccgc
    57421 tcggttgtgt ggagcgccaa acatcaccca gtgcaggtga aagtttcgcg gccagcgggc
    57481 gtacgccctc caggcctgcc agctccagat tactggcgcg aatccggagt tcgtcactgc
    57541 gtttattgtc tttgccgcca acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc
    57601 tcggccaggg tttgccgtcc atcgtgatcc gtgtatcggg aatagagaac tgccagcccg
    57661 gattttcacg cgtaatatgc gcggtcagat tatccaccga cagcgtatgc gtttgcttct
    57721 cgcccaacca gctggcaccg ccctgtttca gccagacgtc accgccggtt acatcgcctt
    57781 tgtcgatcgt catccagcct tcaagggaga actgtgccgt ttccagagca atattgtcct
    57841 gcatccattt accgagccac ggcttcaggt cgatgtcatc cgcctgtagc cagacgcgac
    57901 cattgcttaa caacccctca tcatcgcgca aatccatacg cacctgcatc acgccgtgct
    57961 gtccggtaag gctggagagg cttaccaggc cttccgcacg gtgtcgacgt ggatcgttca
    58021 gccaggtgag ttgtgggatc gccagctcgg cgcgctgacc ggatggcgtc agaaaactga
    58081 cttcactgtc gcggagatcg aaatggtcaa attgacgaag aaacagatcg ctgatgtgac
    58141 tggcttccag gctatcatta ccaccaccgc tggtgatagg agtgttggtg cgaaagcgca
    58201 gctgccagaa agtgaggtcg cgaaactgcc agcgcatatg taacaagctc tgccagacat
    58261 ccagcgccag agtaacgcgt ttaaccgaaa attcgccgcc atcttttagt tctgcacgga
    58321 tgtcgtgtgc ttcaagcgtc gggccaaaat tctgccagct ggctgagagc tgactggctt
    58381 ctaccggcat gccagtcgcg gattctattt tgttgaggat ttccggacgc caggcgtcaa
    58441 gatgcggtaa agcaatacgc aggccgctaa ccagcagggc agcgatcaca acgagcgcgg
    58501 ctccagtaag cagtaaaatc cccggcaatc gcctcacccg tcactccttg tctgctaaaa
    58561 atgtgactca aaaacccttt gccggatggc ggcccagcat ctgtttacat cattacgacg
    58621 tcaaactgct cctggttata gagcggttca atttgtactt taacctgttt gccaacgaaa
    58681 atttccactt ccgccagcga gtgtgactct tcgcctttca aggcttcagc tactgccgga
    58741 gaagcataga ccaggaaacg gtcggagtcg taagcatggt ggacacgaac aatctcgcgc
    58801 atgatttcat agcataccgt ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg
    58861 cattcgttac acagtacgtg ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc
    58921 agccccagcg ccgaaaaacc attaacgctg gttttcaccc ggtctttgct caacgcctgc
    58981 tccagcgagt gcagcactcg gcggcggtga tcttcattat tcatatcgat gaaatcaata
    59041 atgataatcc cgcccagatt gcgcaaccgt aactggcgag cgatagcctg cgtcgcttca
    59101 atattggtat tgaaaatggt gtcgtcaaga ttgcgatgac cgacaaacgc tccggtattg
    59161 atgtccacgg tggtcatcgc ttcggtctgg tcgataatca gataaccacc ggatttcagt
    59221 tctactttgc gttccagcgc tcgctggatt tcgttttcga catcaaagag atcgaaaatc
    59281 ggctggcgtc cggtgtaatg ctccagcttg ctggtcatct cgggaatgta ctccgaggta
    59341 aactccagca atgcttcgta agtcaggcgt gagtcaacgc gaatgcggtc cagttcggcg
    59401 tcggcgaaat cacgcagaac acgctgcgcc agcgccagtt cgccgtacag ctgataacgg
    59461 gtctgcgggc gttttttacg ctccataact ttggtccaga cgcgtttcag ataagcggca
    59521 tcggaggcca gttcagcctc gccaacccct tccgctgcgg tacggatgat aaacccgccc
    59581 tgctcgtcgc aatactctgc gaccactttt ttcaggcgtt cacgttctga ttcgctttca
    59641 atacgttggg aaaccccaac gtgagaagcc cctggcataa acaccagata gcgagaaggc
    59701 agcgtgatat cggtggtcag gcgcgcacct ttagtgccaa gcggatcttt caccacctgc
    59761 accatcagat cttgcccctg acgaaccagt tccgagatgt cgcgcaccgt gaattgcttt
    59821 tgttcttcac ccgccacaca ttcggtgtgc ggcatgatat cggatgcatg aagaaacgcg
    59881 gctttatcca gcccaatatc tacaaaagcc gcctgcatac ccggaagtac acgacttaca
    59941 cgacccttgt agatattgcc tactattccg cgtcgcgcct cacgttcaat atgaatttcc
    60001 tgcagaatac cgccatcaat atacgccact cgcgtttccg aaggcgttac gtttactaac
    60061 aattcagccg tcatgtttat cccttttctc acgcagtgcg ttaaaattac ttaataattc
    60121 atacgtttca accagcggta agccgactac ggcgtgatag ctgccattta tcttcctgac
    60181 aaaacagcca cccagcccct gaataccgta tgcacctgct ttatctaacg gttcaccgct
    60241 ggcgacatag cccgcgatgt cttcgtctgt taacgttctg aaagtcacat cggtgaccac
    60301 caggcaatcg agaatgtgct ggctgtcggc caacgccact gccgtcatca cctgatgggt
    60361 ctgacccgat aatttgcgca acatctgcgc cgcatgctct gcgtcgcgcg gtttctccag
    60421 cacttctcca ttcaggataa cgatagtatc cgcacccagc accgggagat cctgcgccgt
    60481 ttgcgcgaca cccgcctgtg ctttctcgcg cgccagacgc acaacatact gctgcgcgct
    60541 ctcctgcggc tgacgctgct cctcaatgcc cgtaacaata cgttcaaagg tcacgccaag
    60601 ttgcgcaagt aactcctgac gacgcggaga accggaagct aaatacagag aagtcataga
    60661 aacctttatt gcactgcaaa ctgctgacgg actttacgca tcagcaagaa aatccacggc
    60721 cagagcaccc cattgactac actactccag aacacttccg gtctgaaaga gacgttaatc
    60781 actaaaaact ctgcccagaa aacaataata tccaccacca gcgaaagcaa catgacgacc
    60841 agcgcctgct gccataatgc gaggttgcgg aaaagctggt atttcagcgc caccaggtaa
    60901 gcaatgatgc tcatcgccaa tacgcgtacg ccaagcgtcg agccgctgat cagatccagt
    60961 atggcaccca tcacaaaacc tgtgcccaca tttacgcgat gaggcaaggc caggatccaa
    61021 tacaacaaga tgagtaacac ccagtttggc cggaaaacaa tcaggttatc cggccagggc
    61081 atgatttgca gcaacagcgc aatgaggaaa gagagccaga ttacccaacg tccctggcta
    61141 cgatagctcg ccactattgc cctcccggcg cacgcgcagg cggttgagca ccactttgcg
    61201 gcggcgtagc cctttgtgga gagcgattag cagcaggctg tgtgggcgca gcaggcgcag
    61261 tagctgcatt tcctgtcgtc ggttgctgcg gagtcggctg agcgatcccc gttgccggtt
    61321 caggtaactt tggccccatc gcgtctggcg aaggcaatac ctgcggcatc atctgcatca
    61381 gacgttcatt agcaacacga tgcacctctt ccggcgtcat cgggttagcg ccgttacgat
    61441 ccgcgcccca cagcagcagc agataacgca aacgttgcag tcctgcagtc ggacgcgcct
    61501 gaatcacagt ataagcgcgc tgggtatcga gttttacgga agagacaacc gcgaccggat
    61561 agccttccgg gaaacgaccg cccagaccgg aggtcaccag cacatcacca acacgaatat
    61621 ccgtattcgc tggcagatgc tcaagctgca aatcatccgt acaaccgtta ccggctgcaa
    61681 ttacgcggat atcgttgcgc agcacctgga ttggcagcgc gtgggtcgca tcacaaatca
    61741 gcagcacgcg actggtcagt ttagcgacgg ccaccacctg accaacaaca cctttgtcgc
    61801 tgatgaccgg ctggccttca taaacgccat taacgctacc tttatcgata acaacttgat
    61861 cgctataagg atcgttaacc gtggagataa cctgagtcac cattttctgc tcatcctgac
    61921 gcagcgggga acccagcagc tcgcgcagac gcgcgttctc ctgtttgtat tgtccaagca
    61981 tcagcagttc actgtttttc agcaacagtt cctgacgtaa cgcccggttt tcaagttcta
    62041 gttggtcacg caaggccagc gtctgcgata cgccatccag caattcacga ggagcattgg
    62101 aaacaaagta gaaaggactg acggcggtat ccatataagt acggatttga ctgaacgtcc
    62161 ccaggcggct gtcggcaata ataatgccga gcgccaccag caccgccaga ataaggcgaa
    62221 tctgtagcga cgggccacgg ctaaaaattg gcttcataag ttatgcgtat tctcgtatca
    62281 gaccaggcag ggtaaacaga catttcccct gcctgcatcc gattactctt cgctgaacag
    62341 gtcgccgccg tgcatgtcga tcatttccag cgctttgccg ccaccgcgcg ccacacaggt
    62401 cagcgggtct tcagcaacaa cgactggaat gccggtttct tccattaaca aacggtcaag
    62461 gttacgcagc agtgcgccac caccggtgag caccatgccg cgctcggaga tgtcggaagc
    62521 cagttccggc gggcactgtt ccagtgcaac cattaccgcg ctcacaatac cggtcagcgg
    62581 ttcctgcagt gcttcgagga tttcgttgga gttcagggta aaaccgcgtg gaacaccttc
    62641 tgccaggtta cggccacgaa cttcgatttc acggacttca tcgcccgggt aagccgaacc
    62701 gatttcgtgc ttgatacgtt ctgcggtggc ttcaccgatc agagaaccgt aattacgacg
    62761 cacatagttg atgatagctt cgtcgaaacg atcaccacca atgcgcacgg aagaggagta
    62821 aaccacgccg ttcaaggaga taacagcaac ttcagtggta ccaccaccga tatcaaccac
    62881 catagaaccg gtcgcttcag aaaccggcag gccagcacca attgcggcag ccatcggttc
    62941 ttcaatcagg aagacttcac gggcaccagc gccctgcgcg gattcacgaa ttgcgcggcg
    63001 ttcaacctgg gtcgcgccaa ccggtacaca aaccagaacg cgcgggcttg gacgcataaa
    63061 gctgttgctg tgcacttgtt tgatgaagtg ctggagcatt ttttcagtca cgaagaagtc
    63121 ggcgataacg ccgtctttca ttgggcgaat ggcagcaata ttgcccggcg tacggcccag
    63181 catctgcttc gcgtcatgac ctactgcagc tacgcttttc ggtgaaccgg cacgatcctg
    63241 acgaatggcc accacggaag gctcattcaa tacgatgcct tgtcctttta cataaatgag
    63301 ggtattcgca gtacccaggt caatggacaa gtcattggaa aacatgccac gaaatttttt
    63361 caacatacta agggataatc ctgaaagctg gggcggaaaa gaaaatccgc ttactttacc
    63421 aaccacacgc agcagcgaca aggcgcaaaa atcatctgct acggtgaaaa ttagtgcagt
    63481 tcgtttcctt tgttacaaat ctctgcctga gtccagaaag gcttaatgca tcagcagcat
    63541 tcctcgcctg tttgcaaccg cgtaaggtca ttcatctgca tatgtgctgc aacaatctgg
    63601 cgagcagaca agcacactcc catgagacgc agcgcgcgtt attctacgtg aaaacggatt
    63661 aaacggcagg ttaaaccgag tatctttgtg aatatttttt cacgttagta tcaagtggct
    63721 gtgaggacgc gaaaaaatcc ccttgcccgc ctgtaacacc gcgctgaatc agggtctgcc
    63781 actcgcttcg cgaacgcaca ccggtggcgt aaacctgggt gctggtcccg gagcaggctt
    63841 ccaccaggct ttgaaccagc agctggttct ccgttcgctt ctcaatgttt ctgaccagcc
    63901 ccggatggag cttgagtaac tcaacattaa gttctttgat ccaactggtg cttaccagcg
    63961 tcaaaccagc ctggttgacg gctacccgta cccctaaagc atttactaaa cgaataacag
    64021 gttgtaaacg actgatatgt tgacctacat cggcctctgc aagttcaata attatgcgtt
    64081 ttcgttgtga tttttcacat tgcattaacg tatcgcgcag ccaacgctga aaacgcgggc
    64141 gaatcagcga ctcaacggta acctgaatcg ccagattttc ctctggccag taacgcaata
    64201 gtggaataag acggctgatt tgcagacggt catactcttc cgataagcca aactgcaaga
    64261 ccatcggcat atactccgcc gagctaacct cttcattacc atcgaagatg cggcacatga
    64321 gttcgcgatg atgaacctga ccttcgcgag taaccgccgg tttttgataa aggcgcgggc
    64381 cgccgcgact gagcatttgc tcgataagcg tacgccagcg aacattaccg cgtccttttt
    64441 caggcaacga gtcatcgtaa atagcccagc tattgccgcc ctgcaatccc gcattacgcg
    64501 tggcagactc tgcatgttcc attacctgct cggtatcctg accactacgc caggcgcaga
    64561 taccaatgtg gatcatatcg tcgcgatcga gcattttatt gttcggcaag gtatcgacgg
    64621 ctttgattaa ctgaccggcg atgctctctg cctcttttaa cgtccggtgc ggtaacagcg
    64681 cagcaaaatc actgcggtgg taacgcgcca gcagtgcgcc agggtagcgc atcataaatg
    64741 tcgacagcag attcgtcaga gtgaagaact gttcttcaac ctggctgtgc ccccaagtat
    64801 cgctcaacat attgaaatcc ggcagacgaa tcatcatcac gatcccgtgg gtacctactt
    64861 tctcctgatc ttccagtaac gttgctaact gattatcgaa aaagagtcgg ttattgaggc
    64921 cggttttcac gtcctgggcg gcataagagc ggatcagcgt atcaagacgg ctgtgttgtt
    64981 cgcgtgcgtt ctgaatttca cgaagcagcg tatctagcgc actgctggtt ctgggcggcc
    65041 attcatagat ggttcccaac acattagagc cacgctcacc gtttaagata cgagtagccc
    65101 gggtttccag caattcttgc ccggcaagtt gccgttgtaa ccagcgtacc gccaggaaga
    65161 gcataacaat gataaagcca atcgcccccg tgagcggcgc ggtagtcatt aaagaatgga
    65221 aatagttgcc catcggatcc tgataaacca gacgcagcga catccccgga tgctttatca
    65281 acggaacact cagttcgcga aacagatcgc tggagccaac cggacgataa ctaccatttc
    65341 tggccagggt ataaacctgt ttatcaccat ggagcaggtc tacacgaacg atatctgccg
    65401 acatcattaa ttcggtaatt tgtggcctta atgtgctgaa gtcattcgac acaaggtggg
    65461 tatcaatcgc cgtcgccacc gcctgaacgc gatgactaaa cttatactga attgcgttgt
    65521 agaaacttag cgaacagccc agcaaagtca caaaaattgt taacccggtg agcagcgtaa
    65581 caaaggccga aaatttcgtc gttaatctca tccttgtgtt aactccgata gtgaggaagc
    65641 gggcatacta gcaaatcaga tttatctcgc aatttattgc gcttcatcgg ctttgctttt
    65701 ccattagcga gtatagtctt cagaaattat tttccaatcc atcatgcaca tgaggaccac
    65761 ttatgcaggc gttactttta gaacagcagg acggcaaaac tctcgcatcg gtacagacac
    65821 ttgacgaaag tcgcctgccg gagggcgatg tcacggttga tgttcactgg tcgagcctga
    65881 actataaaga tgcgctggcg attaccggta agggaaaaat catccgtaat tttccgatga
    65941 ttcctgggat cgattttgcc ggaactgtac gcaccagcga agatccgcgt tttcatgccg
    66001 gtcaggaggt gttactcact ggctggggcg ttggtgaaaa ccactggggt gggctggcgg
    66061 agcaggcgcg agtgaaaggt gactggctgg ttgccatgcc gcaagggctg gacgcgcgta
    66121 aagcaatgat tatcggtact gccggtttta ccgccatgct gtgtgtgatg gcgctggaag
    66181 atgccggtgt tcgcccgcag gacggggaga ttgtcgtgac gggtgccagt ggtggcgtcg
    66241 gcagtaccgc cgtggcgctg ctgcataagt tgggttatca ggtcgttgcc gtttccggtc
    66301 gcgaaagtac ccatgaatat ctgaaaagtt taggtgccag tcgtattctc cctcgtgatg
    66361 agtttgccga atcccgtcct ctggaaaaac aagtctgggc tggggcaatt gacactgttg
    66421 gcgacaaagt gctggcaaaa gtgctggcgc aaatgaatta cggcggctgc gtggctgcct
    66481 gtggtctggc gggtggtttt actctgccaa ccacggtcat gccatttatt ctgcgtaatg
    66541 tccgtttgca aggggtggat tcagtaatga cgccaccaga acgccgcgca caagcctggc
    66601 agcgactggt cgccgattta ccggaatcat tctataccca ggcggcaaaa gagatatctc
    66661 tgtcagaggc accgaacttt gccgaggcca tcattaataa ccagatccag ggtcgcacgc
    66721 tggtgaaggt taactaacca tttagcaggg aataataaga gagggaactc attttgaaat
    66781 cattattcaa tcggttaacg ggaaaagcgg ttagccggac agctttcgtc gaacaccttg
    66841 gtcagaaagt tatacaacat catccaaact gtaaagtcat gatttcgact gaccacaaat
    66901 tgatgcgcat tgatactcca ctaaacagct attattgata cgcctccgtc gcctgttagg
    66961 tttatggtgc tttgcctggg cgacgctacg cttagcccct tacttatttc tggtaccatg
    67021 gggtgaataa tctgatttta tttgactaca aattaaccac tcaaacctat ttaatgctga
    67081 gcattgtcag tcggttgatt ttgcgtgctt tagcattcac gtctatccag acgatgcagt
    67141 gaaaattggg taatcctcag caaccgctgc gtaatgtcgt ctatcttgtc gcgatcctgg
    67201 catccctaca ttatttgtgg tctgtgaaga ttatctcatt gcagcccctc atcttcacag
    67261 ggctggcttt acagtttttc accttacgtt ataagaagtt ccgtcgatga tggcgctaat
    67321 ttcgtgaatt gtgcggcttg ttgcaaatta cacggtgttg aaggttattt acatgttagc
    67381 tgttgattat cttccctgat aagaccagta tttagctgcc aattgctacg aaatcgttat
    67441 aatgtgcgac ctcgtcctcc ctgacgcagt ttttgcgctg cggaaaaggt gacattggcg
    67501 caacgaaggt atattttgtt ttttgccgga ggatagcagc agatcgctgc acaatgtccg
    67561 tcaagtctaa cattgacact ctggggcaaa atagaccggc gtcccggcct gctggaattt
    67621 atcgctatgc atacagctgt cggggcatac gctttacaga cggcggtgaa acgcctgtca
    67681 caatcacact aaacaaagag tacggaaccc actcatggat attcgtaaga ttaaaaaact
    67741 gatcgagctg gttgaagaat caggcatctc cgaactggaa atttctgaag gcgaagagtc
    67801 agtacgcatt agccgtgcag ctcctgccgc aagtttccct gtgatgcaac aagcttacgc
    67861 tgcaccaatg atgcagcagc cagctcaatc taacgcagcc gctccggcga ccgttccttc
    67921 catggaagcg ccagcagcag cggaaatcag tggtcacatc gtacgttccc cgatggttgg
    67981 tactttctac cgcaccccaa gcccggacgc aaaagcgttc atcgaagtgg gtcagaaagt
    68041 caacgtgggc gataccctgt gcatcgttga agccatgaaa atgatgaacc agatcgaagc
    68101 ggacaaatcc ggtaccgtga aagcaattct ggtcgaaagt ggacaaccgg tagaatttga
    68161 cgagccgctg gtcgtcatcg agtaacgagg cgaacatgct ggataaaatt gttattgcca
    68221 accgcggcga gattgcattg cgtattcttc gtgcctgtaa agaactgggc atcaagactg
    68281 tcgctgtgca ctccagcgcg gatcgcgatc taaaacacgt attactggca gatgaaacgg
    68341 tctgtattgg ccctgctccg tcagtaaaaa gttatctgaa catcccggca atcatcagcg
    68401 ccgctgaaat caccggcgca gtagcaatcc atccgggtta cggcttcctc tccgagaacg
    68461 ccaactttgc cgagcaggtt gaacgctccg gctttatctt catcggcccg aaagcagaaa
    68521 ccattcgcct gatgggcgac aaagtatccg caatcgcggc gatgaaaaaa gcgggcgttc
    68581 cttgcgtacc tggttctgac ggcccgctgg gcgacgatat ggataaaaac cgcgccattg
    68641 ctaaacgcat tggttatccg gtgattatca aagcctccgg cggcggcggc ggtcgcggta
    68701 tgcgcgtagt gcgcggcgac gctgaactgg cacaatccat ctccatgacc cgtgcggaag
    68761 cgaaagctgc tttcagcaac gatatggttt acatggagaa atacctggaa aatcctcgcc
    68821 acgtcgagat tcaggtactg gctgacggtc agggcaacgc tatctatctg gcggaacgtg
    68881 actgctccat gcagcgccgc caccagaaag tggtcgaaga agcgccagca ccgggcatta
    68941 ccccggaact gcgtcgctac atcggcgaac gttgcgctaa agcgtgtgtt gatatcggct
    69001 atcgcggtgc aggtactttc gagttcctgt tcgaaaacgg cgagttctat ttcatcgaaa
    69061 tgaacacccg tattcaggta gaacacccgg ttaccgaaat gatcaccggc gttgacctga
    69121 tcaaagaaca gctgcgtatc gctgccggtc aaccgctgtc gatcaagcaa gaagaagttc
    69181 acgttcgcgg ccatgcggtg gaatgtcgta tcaacgccga agatccgaac accttcctgc
    69241 caagcccagg caaaatcacc cgtttccacg cacctggcgg ttttggcgta cgttgggagt
    69301 ctcatatcta cgcgggctac accgtaccgc cgtactatga ctcaatgatc ggtaagctga
    69361 tttgctacgg tgaaaaccgt gacgtggcga ttgcccgcat gaagaatgcg ctgcaggagc
    69421 tgatcatcga cggtatcaaa accaatgttg atctgcagat ccgcatcatg aatgacgaga
    69481 acttccagca tggtggcact aacatccact atctggagaa aaaactcggt cttcaggaaa
    69541 aataagactg caaaagcgtc aaaaggccgg attttccggc cttttttatt actggggatc
    69601 gacaaccccc ataaggtaca atcctcgctt tcttcaccca tcagggacaa aaaatggaca
    69661 ctcgttttgt tcaggcccat aaagaggcgc gctgggcgct ggggctgacc cttttgtatc
    69721 tggcagtttg gttagtagcc gcttacttac ctggcgttgc ccccggtttt accggctttc
    69781 cgcgctggtt tgagatggcc tgcatcctga cgccgctact gtttattgga ctgtgctggg
    69841 cgatggtgaa atttatctat cgcgatatcc cactggagga tgacgatgca gcttgaagta
    69901 attctaccgc tggtcgccta tctggtggtg gtgttcggta tctcggttta tgcgatgcgt
    69961 aaacggagca ccggcacctt ccttaatgag tatttcctcg gcagccgctc tatgggcggt
    70021 attgtgctgg cgatgacgct caccgcgacc tatatcagtg ccagttcgtt tatcggcggg
    70081 cctggagctg cttataaata cggcctgggt tgggtattgc tggcgatgat tcagcttcct
    70141 gcagtctggc tttcgctcgg tattctcggc aagaagttcg cgattcttgc gcgccgctac
    70201 aatgcagtga cgctgaacga tatgctgttt gcccgctacc agagtcgtct tctggtgtgg
    70261 ctggcgagtt tgagtttgct ggttgcgttc gttggggcaa tgaccgtgca gtttatcggc
    70321 ggtgcgcgcc tgctggaaac cgcggcgggt attccttatg aaaccgggct gctgattttc
    70381 ggtatcagca ttgcgttgta taccgccttt ggcggctttc gcgccagcgt gctgaacgac
    70441 accatgcaag ggcttgtgat gctgattggc accgttgtgc tgcttattgg cgtggtacat
    70501 gccgctggcg gcttaagcaa cgcagtacag accttgcaaa ccatcgatcc gcaactggtt
    70561 acgccacaag gcgctgacga tattctgtcg cctgccttta tgacgtcgtt ctgggtactg
    70621 gtgtgctttg gcgtgattgg cctgccgcat actgcggtgc gctgtatctc ttataaagac
    70681 agcaaagcag tacaccgggg gatcatcatc ggtacgattg tagtcgcaat cctgatgttc
    70741 ggtatgcacc tggccggagc gttaggtcgt gcagtgatcc ccgatctcac cgtaccggat
    70801 ctggtgatcc cgacgttaat ggtaaaagtg ctgccaccgt ttgctgccgg gatctttctg
    70861 gctgcaccta tggctgcgat catgtcgaca attaacgccc aattgctaca aagttccgct
    70921 acgatcatta aagatctcta tctgaatatc cgtccggatc aaatgcaaaa cgagacgcgt
    70981 ctgaagcgga tgtcggcggt aattacgtta gttctcggcg cgttgctgct gcttgccgcc
    71041 tggaagccgc cagaaatgat catctggctg aatttgctgg ccttcggtgg gctggaagcc
    71101 gttttcctgt ggccgctggt gctgggtctt tactgggaac gcgccaacgc caaaggcgcg
    71161 ctaagtgcga tgatcgttgg cggcgtgctg tatgccgtac tcgcgacgct gaatattcag
    71221 tacctgggct tccaccctat cgtgccctcg ttactactaa gtttggtggc tttcctggtc
    71281 ggaaaccgtt tcggtacatc cgtcccgcaa gctaccgttt tgactactga taaataaaga
    71341 gttttgccat gccttggatc caactgaaac tgaacaccac cggcgcgaac gcggaagatc
    71401 ttagcgatgc gctgatggaa gcgggtgccg tttctatcac ttttcaggat acccacgata
    71461 cgccagtatt tgagccgctg ccgggcgaaa cgcgcctgtg gggcgacacc gatgtgattg
    71521 gtctgttcga cgctgaaacc gatatgaacg acgtggtggc gattctggaa aaccatccgc
    71581 tgctcggcgc aggcttcgcg cataaaatcg aacaactaga agataaagac tgggagcgcg
    71641 aatggatgga taatttccac ccgatgcgct ttggtgaacg actgtggatc tgccctagct
    71701 ggcgtgatgt gccggacgaa aacgccgtca acgtgatgtt agatccaggg ctggcgtttg
    71761 gtacgggtac ccatccaacc acctctctgt gcctgcaatg gctcgacagc ctcgatttaa
    71821 ccggtaaaac agtcatcgac tttggctgtg gttccggcat tctggcgatc gcggcgctga
    71881 aactgggtgc agcaaaagcc attggtattg atatcgatcc gcaggcgatt caggccagcc
    71941 gcgacaacgc cgaacgtaat ggcgtttctg accgtctgga actctactta ccgaaagatc
    72001 agccagaaga aatgaaagcc gacgtggtgg tcgctaacat ccttgcaggc ccattacgtg
    72061 aactggcacc gttaatcagc gtcctgccgg tttcaggcgg tttgctgggc ctttccggta
    72121 ttctggcaag ccaggcagag agcgtttgtg aagcttatgt cgacagcttc gcactggacc
    72181 cggtcgtgga aaaagaagag tggtgccgca ttaccggtcg taagaattaa ccttcgcatc
    72241 gccgtagggt gacgcggggc gaagtgcgag caagctcaca aaaggcacgt aaatttgccg
    72301 attatttacg caaatttgcg tgccaaaatt ttcattcata aagaaaaatt gagaacttac
    72361 tcaaatttct tt
//
LOCUS       DAPHJE010000006        65814 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPHJE010000006 DAPHJE010000000
VERSION     DAPHJE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.006"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            186..1085
                     /gene="lysO"
                     /locus_tag="RH222_000452"
     CDS             186..1085
                     /gene="lysO"
                     /locus_tag="RH222_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV3795518.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1229..2881
                     /gene="hcp"
                     /locus_tag="RH222_000453"
                     /gene_synonym="priS"
     CDS             1229..2881
                     /gene="hcp"
                     /locus_tag="RH222_000453"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV3795519.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2893..3861
                     /gene="hcr"
                     /locus_tag="RH222_000454"
     CDS             2893..3861
                     /gene="hcr"
                     /locus_tag="RH222_000454"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV3795520.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            3994..5712
                     /gene="poxB"
                     /locus_tag="RH222_000455"
     CDS             3994..5712
                     /gene="poxB"
                     /locus_tag="RH222_000455"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV3795521.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5749..6750
                     /gene="ltaE"
                     /locus_tag="RH222_000456"
     CDS             5749..6750
                     /gene="ltaE"
                     /locus_tag="RH222_000456"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV3795522.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6761..8191
                     /locus_tag="RH222_000457"
     CDS             6761..8191
                     /locus_tag="RH222_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV3795523.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8290..9303
                     /locus_tag="RH222_000458"
     CDS             8290..9303
                     /locus_tag="RH222_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV3795524.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9300..10130)
                     /gene="amiD"
                     /locus_tag="RH222_000459"
     CDS             complement(9300..10130)
                     /gene="amiD"
                     /locus_tag="RH222_000459"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV3795525.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTVSL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10127..10450)
                     /locus_tag="RH222_000460"
     CDS             complement(10127..10450)
                     /locus_tag="RH222_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV3795526.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10576..11091
                     /locus_tag="RH222_000461"
     CDS             10576..11091
                     /locus_tag="RH222_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3795527.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKKGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11309..12037
                     /gene="artP"
                     /locus_tag="RH222_000462"
     CDS             11309..12037
                     /gene="artP"
                     /locus_tag="RH222_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV3795528.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12055..12786
                     /gene="artJ"
                     /locus_tag="RH222_000463"
     CDS             12055..12786
                     /gene="artJ"
                     /locus_tag="RH222_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3795529.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            12793..13509
                     /gene="artQ"
                     /locus_tag="RH222_000464"
     CDS             12793..13509
                     /gene="artQ"
                     /locus_tag="RH222_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV3795530.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13509..14177
                     /gene="artM"
                     /locus_tag="RH222_000465"
     CDS             13509..14177
                     /gene="artM"
                     /locus_tag="RH222_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV3795531.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            14469..15200
                     /gene="artJ"
                     /locus_tag="RH222_000466"
     CDS             14469..15200
                     /gene="artJ"
                     /locus_tag="RH222_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV3795532.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(15375..16502)
                     /gene="rlmC"
                     /locus_tag="RH222_000467"
     CDS             complement(15375..16502)
                     /gene="rlmC"
                     /locus_tag="RH222_000467"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV3795533.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16543..17031)
                     /locus_tag="RH222_000468"
     CDS             complement(16543..17031)
                     /locus_tag="RH222_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV3795534.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(17091..17936)
                     /gene="potI"
                     /locus_tag="RH222_000469"
     CDS             complement(17091..17936)
                     /gene="potI"
                     /locus_tag="RH222_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV3795535.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(17933..18886)
                     /gene="potH"
                     /locus_tag="RH222_000470"
     CDS             complement(17933..18886)
                     /gene="potH"
                     /locus_tag="RH222_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV3795536.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLSEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(18896..20029)
                     /gene="potG"
                     /locus_tag="RH222_000471"
     CDS             complement(18896..20029)
                     /gene="potG"
                     /locus_tag="RH222_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV3795537.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(20124..21236)
                     /gene="potF"
                     /locus_tag="RH222_000472"
     CDS             complement(20124..21236)
                     /gene="potF"
                     /locus_tag="RH222_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV3795538.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(21587..22063)
                     /locus_tag="RH222_000473"
     CDS             complement(21587..22063)
                     /locus_tag="RH222_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV3795539.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(22151..23053)
                     /gene="rimK"
                     /locus_tag="RH222_000474"
     CDS             complement(22151..23053)
                     /gene="rimK"
                     /locus_tag="RH222_000474"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV3795540.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(23114..23836)
                     /gene="nfsA"
                     /locus_tag="RH222_000475"
     CDS             complement(23114..23836)
                     /gene="nfsA"
                     /locus_tag="RH222_000475"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV3795541.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(23820..24107)
                     /locus_tag="RH222_000476"
     CDS             complement(23820..24107)
                     /locus_tag="RH222_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV3795542.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            24267..24524
                     /gene="grxA"
                     /locus_tag="RH222_000477"
     CDS             24267..24524
                     /gene="grxA"
                     /locus_tag="RH222_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV3795543.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(24554..24931)
                     /locus_tag="RH222_000478"
     CDS             complement(24554..24931)
                     /locus_tag="RH222_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV3795544.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            25201..26886
                     /locus_tag="RH222_000479"
     CDS             25201..26886
                     /locus_tag="RH222_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV3795545.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(27061..27597)
                     /gene="rcdA"
                     /locus_tag="RH222_000480"
     CDS             complement(27061..27597)
                     /gene="rcdA"
                     /locus_tag="RH222_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV3795546.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            27681..28889
                     /locus_tag="RH222_000481"
     CDS             27681..28889
                     /locus_tag="RH222_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3795547.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            28889..29704
                     /gene="ybjI"
                     /locus_tag="RH222_000482"
     CDS             28889..29704
                     /gene="ybjI"
                     /locus_tag="RH222_000482"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV3795548.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            29790..30074
                     /locus_tag="RH222_000483"
     CDS             29790..30074
                     /locus_tag="RH222_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795549.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(30115..31347)
                     /gene="mdfA"
                     /locus_tag="RH222_000484"
     CDS             complement(30115..31347)
                     /gene="mdfA"
                     /locus_tag="RH222_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV3795550.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            31632..32228
                     /gene="ybjG"
                     /locus_tag="RH222_000485"
     CDS             31632..32228
                     /gene="ybjG"
                     /locus_tag="RH222_000485"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV3795551.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            32286..33044
                     /gene="deoR"
                     /locus_tag="RH222_000486"
     CDS             32286..33044
                     /gene="deoR"
                     /locus_tag="RH222_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV3795552.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(33091..34293)
                     /gene="dacC"
                     /locus_tag="RH222_000487"
     CDS             complement(33091..34293)
                     /gene="dacC"
                     /locus_tag="RH222_000487"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV3795553.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            34540..35166
                     /gene="gstB"
                     /locus_tag="RH222_000488"
     CDS             34540..35166
                     /gene="gstB"
                     /locus_tag="RH222_000488"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV3795554.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(35163..36278)
                     /gene="yliI"
                     /locus_tag="RH222_000489"
     CDS             complement(35163..36278)
                     /gene="yliI"
                     /locus_tag="RH222_000489"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV3795555.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(36389..36772)
                     /gene="bssR"
                     /locus_tag="RH222_000490"
     CDS             complement(36389..36772)
                     /gene="bssR"
                     /locus_tag="RH222_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV3795556.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            36985..38310
                     /gene="rimO"
                     /locus_tag="RH222_000491"
     CDS             36985..38310
                     /gene="rimO"
                     /locus_tag="RH222_000491"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV3795557.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(38357..39685)
                     /locus_tag="RH222_000492"
     CDS             complement(38357..39685)
                     /locus_tag="RH222_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV3795558.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFEEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(39693..42041)
                     /locus_tag="RH222_000493"
     CDS             complement(39693..42041)
                     /locus_tag="RH222_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3795559.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(42219..43130)
                     /gene="gsiD"
                     /locus_tag="RH222_000494"
     CDS             complement(42219..43130)
                     /gene="gsiD"
                     /locus_tag="RH222_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV3795560.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(43133..44053)
                     /gene="gsiC"
                     /locus_tag="RH222_000495"
     CDS             complement(43133..44053)
                     /gene="gsiC"
                     /locus_tag="RH222_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV3795561.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(44071..45609)
                     /gene="gsiB"
                     /locus_tag="RH222_000496"
     CDS             complement(44071..45609)
                     /gene="gsiB"
                     /locus_tag="RH222_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV3795562.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(45629..47500)
                     /gene="gsiA"
                     /locus_tag="RH222_000497"
     CDS             complement(45629..47500)
                     /gene="gsiA"
                     /locus_tag="RH222_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV3795563.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSCKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(47487..48452)
                     /gene="iaaA"
                     /locus_tag="RH222_000498"
     CDS             complement(47487..48452)
                     /gene="iaaA"
                     /locus_tag="RH222_000498"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV3795564.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            48656..49891
                     /gene="moeA"
                     /locus_tag="RH222_000499"
     CDS             48656..49891
                     /gene="moeA"
                     /locus_tag="RH222_000499"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV3795565.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            49891..50640
                     /gene="moeB"
                     /locus_tag="RH222_000500"
     CDS             49891..50640
                     /gene="moeB"
                     /locus_tag="RH222_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV3795566.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(50716..51378)
                     /gene="fsa"
                     /locus_tag="RH222_000501"
     CDS             complement(50716..51378)
                     /gene="fsa"
                     /locus_tag="RH222_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV3795567.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            51509..52408
                     /locus_tag="RH222_000502"
     CDS             51509..52408
                     /locus_tag="RH222_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV3795568.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            52414..54846
                     /locus_tag="RH222_000503"
     CDS             52414..54846
                     /locus_tag="RH222_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV3795569.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRDQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            54992..55807
                     /gene="ybiV"
                     /locus_tag="RH222_000504"
     CDS             54992..55807
                     /gene="ybiV"
                     /locus_tag="RH222_000504"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV3795570.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            55959..57224
                     /locus_tag="RH222_000505"
     CDS             55959..57224
                     /locus_tag="RH222_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV3795571.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(57465..59057)
                     /locus_tag="RH222_000506"
     CDS             complement(57465..59057)
                     /locus_tag="RH222_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV3795572.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            59276..60196
                     /gene="ldtB"
                     /locus_tag="RH222_000507"
     CDS             59276..60196
                     /gene="ldtB"
                     /locus_tag="RH222_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3795573.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(60255..61373)
                     /locus_tag="RH222_000508"
     CDS             complement(60255..61373)
                     /locus_tag="RH222_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV3795574.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(61370..61837)
                     /gene="mntR"
                     /locus_tag="RH222_000509"
     CDS             complement(61370..61837)
                     /gene="mntR"
                     /locus_tag="RH222_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV3795575.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            62023..62151
                     /gene="mntS"
                     /locus_tag="RH222_000510"
     CDS             62023..62151
                     /gene="mntS"
                     /locus_tag="RH222_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV3795576.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            62423..64006
                     /gene="opgE"
                     /locus_tag="RH222_000511"
     CDS             62423..64006
                     /gene="opgE"
                     /locus_tag="RH222_000511"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3795577.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(64055..64570)
                     /gene="ompX"
                     /locus_tag="RH222_000512"
     CDS             complement(64055..64570)
                     /gene="ompX"
                     /locus_tag="RH222_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV3795578.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(64623..64688)
                     /locus_tag="RH222_000513"
     CDS             complement(64623..64688)
                     /locus_tag="RH222_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795579.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            64923..65810
                     /gene="rhtA"
                     /locus_tag="RH222_000514"
     CDS             64923..65810
                     /gene="rhtA"
                     /locus_tag="RH222_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV3795580.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT        16146 a        17393 c        16369 g        15906 t
ORIGIN      
        1 attttgacgc caccaacatc ggtgcagttt gtaattaaag cgcaagaata tctttaaatg
       61 aaaacgaata ctctactgct ccttatgcat aacatttcct tttaattgca actttactcg
      121 tccttccgct ttcagtagtg tgtcatcgcg catttcgact tcatctttgc aaggactggg
      181 ttttcatgtt ttctgggctg ttaatcattc tggttcccct gattgtgggt tacctcattc
      241 cgcttcgcca acaagctgcg ttaaaagtta ttaatcagct attaagctgg atggtttacc
      301 ttattctctt ttttatgggt atcagtctgg cgtttctcga taacctcgcc agtaacctgt
      361 tggcgattct gcattattct gccgtcagta ttaccgttat tttactgtgt aatattgccg
      421 ccctgatgtg gctggagcga ggcctgccgt ggcgcaacca ccatcagcaa gaaaaactcc
      481 cgtcgcgtat tgcgatggcg ctggagtcgc taaaactgtg cggcgtagta gtgattggtt
      541 ttgccattgg tctgagtgga ctggctttct tacaacacgc gaccgaagcc agtgaataca
      601 cgttaatttt gctacttttc ctcgttggta ttcagttgcg caataatggc atgaccttaa
      661 agcagattgt ccttaatcgc cggggaatga ttgtcgccgt ggtggtggtt gtcagttcat
      721 taattggtgg tttaattaac gcctttattc ttgatctccc catcaatacc gcgctggcaa
      781 tggcctccgg tttcggctgg tattctcttt ccggtatttt attgaccgaa tcttttggtc
      841 cggtaatcgg gagcgcggcg ttttttaatg atctggcccg tgaactgatt gctattatgt
      901 tgatccctgg gctgattcgc cgcagccgct ctactgcact gggcttatgc ggtgccacat
      961 caatggattt caccctgccc gttcttcaac gtactggcgg gctggatatg gtcccggcgg
     1021 caattgttca cggttttatt cttagcctgt tagtgccgat cctcatcgcc tttttctctg
     1081 cgtaatacct ctctggcggt agatccctgc cgccaaaatt gcgctaaatc aatctccctt
     1141 aaagttgcat gaaaaatccc ttttatcccc gcgttaagcg tcttaacctt aaacatgtat
     1201 attaaatata actttaaaag gtgtgatcat gttttgtgtg caatgtgaac aaactatccg
     1261 tactccggca ggaaacggct gctcatacgc gcaggggatg tgtggtaaaa cggcggaaac
     1321 ttctgacctt caggatttac tcatcgcggc gctgcaaggg ctttctgcct gggcggtaaa
     1381 agcgcgtgaa tacggcatca tcaaccacga tgttgacagc ttcgcgccac gtgccttttt
     1441 ctcaaccctg accaacgtta acttcgattc tccgcgtatt gtcggctacg ctcgtgaagc
     1501 gattgccctg cgcgaggcgc tgaaagcgca atgcctggct gtagatgcca acgcgcgcgt
     1561 cgataacccg atggctgacc tgcaactggt gagcgacgat ctcggcgagc tgcaacgtca
     1621 ggcagcagaa tttaccccta acaaagataa agcggcgatt ggcgaaaaca ttctcggcct
     1681 gcgtctgctg tgcctgtatg gcctgaaagg tgcagcggcc tatatggaac acgcgcatgt
     1741 tctcggtcaa tacgacaacg atatttatgc ccagtatcat aaaatcatgg cgtggctggg
     1801 gacctggcct gccgatatga acgcgcttct tgagtgttca atggaaatcg gccagatgaa
     1861 cttcaaagtg atgagcattc tggatgcagg cgaaaccggt aaatacggtc acccgacgcc
     1921 aacccaggtc aacgtcaaag cgacggcggg taaatgcatc ctgatttccg gtcacgatct
     1981 caaagatctc tacaacctgc tggaacagac cgaaggcacg ggcgttaatg tttacaccca
     2041 cggcgaaatg ctgcctgcgc atggctaccc ggagctgcgt aaattcaagc atctggtcgg
     2101 taactacggc agcggctggc agaatcagca agtggagttc gctcgtttcc ctggccccat
     2161 cgtgatgacc tcgaactgca tcatcgaccc aaccgtaggc gcttatgacg atcgtatctg
     2221 gacccgcagc atcgttggct ggccgggtgt acgtcatctg gatggcgaag atttttctgc
     2281 ggtcatcgcc caggcgcaac aaatggcggg cttcccgtac agcgaaattc cgcacctgat
     2341 caccgtgggc tttggccgcc agacgctgct tggcgcagcg gatacgctga ttgatctggt
     2401 gagccgtgaa aaactgcgtc atatcttcct gcttggtggc tgtgacggcg cacgcggcga
     2461 gcgccactac ttcaccgatt tcgccaccag cgtgccggat gactgcctga tcctgaccct
     2521 cgcctgtggt aaatatcgct ttaacaaact tgagtttggc gatatcgaag gtctgccgcg
     2581 tctggtagat gcaggtcaat gtaacgatgc ttactcagcg attattctgg ctgtcactct
     2641 ggcagagaaa ctgggctgcg gtgtcaacga tctgccgctg tcgctggtgc tctcctggtt
     2701 tgaacagaaa gcgatcgtca ttctgctgac gctgctttct ctgggcgtga aaaatatcgt
     2761 caccggcccg actgcacctg gtttcctgac accagacctg ctggcggtgc tgaacgagaa
     2821 atttggcctg cgttctatca ccactgttga agaagacatg aagcaactgt tgagcgcgta
     2881 aggagtttgt cgatgacgat gccaacgaat caatgcccgt ggcggatgca agttcatcac
     2941 attacgcaag aaacgccgga tgtgtggacg atttccctga tttgccacga ttactaccca
     3001 tatcgcgccg ggcaatatgc actggtcagc gtgcgaaact cagcggaaac gctgcgtgct
     3061 tacaccattt cctccacgcc aggcgtgagt gaatatatca ccctgaccgt gcggcggatt
     3121 gatgacggtg tcggctccca gtggctgacg cgcgatgtaa aacgcggtga ttatctctgg
     3181 ctttcggacg cgatggggga atttacctgc gacgataaag cagaagataa attcctgttg
     3241 ctggcggcag gctgcggcgt cacgccgatt atgtcgatgc gtcgctggct tgcgaagaac
     3301 cgtccacagg ccgatgtgcg ggtgatctac aacgtgcgta cgccgcagga tgttattttc
     3361 gccgatgagt ggcgtaacta tccggtaacg ctggtggcag aaaataacgt taccgaaggc
     3421 tttatcgctg gtcgtctcac tcgcgaactg ctggcaggtg tacctgactt agcttcacgt
     3481 accgtgatga cctgcggccc ggctccgtat atggattggg tagagcagga agtgaaagcg
     3541 ctcggcgtga cgcgtttctt taaagagaaa ttcttcaccc cagtagcaga agcggcgacc
     3601 agcggtctga aattcaccaa actgcaaccg gcacgagaat tttacgcccc ggttggcacc
     3661 acgctactgg aggcgctgga aagcaataac gttccggttg tcgctgcctg ccgtgcgggt
     3721 gtttgcggct gctgtaagac aaaagtggtt tccggtgaat atacggtgag cagtacaatg
     3781 acgctgaccg acgccgaaat cgctgaaggt tacgtactgg cctgctcctg ccatccgcag
     3841 ggggatttgg ttctcgcata atcgccttat acccgatgat attcctttca tcgggctatt
     3901 taaccgttag tgcctccttt ctctcccatc ccttccccct ccgtcagatg aactaaactt
     3961 gttaccgtta tcacattcag gagatggaga accatgaaac aaacggttgc agcttatatc
     4021 gccaaaacac tcgaatcggc aggggtgaaa cgcatctggg gagtcactgg cgactctctg
     4081 aacggtctta gtgacagtct taatcgcatg ggcaccatcg agtggatgtc cacccgccac
     4141 gaagaagtgg cggcctttgc cgctggcgct gaagcacaac ttagcggaga actggcggtc
     4201 tgcgccggat cgtgcggccc cggcaacctg cacttaatca acggcctgtt cgattgccac
     4261 cgcaatcacg ttccggtact ggcgattgcc gctcatattc cctccagcga aattggcagc
     4321 ggctatttcc aggaaaccca cccacaagag ctattccgcg aatgtagtca ctattgcgag
     4381 ctggtttcca gcccggagca gatcccacaa gtactggcga ttgccatgcg caaagcggtg
     4441 cttaaccgtg gcgtttcggt tgtcgtgtta ccaggcgacg tggcgttaaa acctgcgcca
     4501 gaaggggcaa ccacgcactg gtatcatgcg ccacaaccag tcgtgacgcc ggaagaagaa
     4561 gagttacgca aactggcgca actgctgcgt tattccagca atatcgccct gatgtgtggc
     4621 agcggctgcg cgggggcgca taaagagtta gttgagtttg ccgggaaaat taaagcgcct
     4681 attgttcatg ccctgcgcgg taaagaacat gtcgaatacg ataatccgta tgatgttgga
     4741 atgaccgggt taatcggctt ctcgtcaggt ttccatacca tgatgaacgc cgacacgtta
     4801 gtgctactcg gcacgcaatt tccctaccgc gccttctacc cgaccgatgc caaaatcatt
     4861 cagattgata tcaacccagc cagcatcggc gctcacagca aggtggatat ggcactggtc
     4921 ggcgatatca agtcgactct gcgtgcattg cttccattgg tggaagaaaa agccgatcgc
     4981 aagtttctgg ataaagcgct ggaagattac cgcgacgccc gcaaagggct ggacgattta
     5041 gctaaaccga gcgagaaagc cattcacccg caatatctgg cgcagcaaat tagtcatttt
     5101 gccgccgatg acgctatttt cacctgtgac gttggtacgc caacggtgtg ggcggcacgt
     5161 tatctaaaaa tgaacggcaa gcgtcgcctg ttaggttcgt ttaaccacgg ttcgatggct
     5221 aacgccatgc cgcaggcgct gggtgcgcag gcgacagagc cagaacgtca ggtggtcgcc
     5281 atgtgcggcg atggcggttt tagcatgttg atgggcgatt tcctctcagt agtgcagatg
     5341 aaactgccag tgaaaattgt cgtctttaac aacagcgtgc tgggctttgt ggcgatggag
     5401 atgaaagctg gtggctattt gactgacggc accgaactac acgacacaaa ctttgcccgc
     5461 attgccgaag cgtgcggcat tacgggtatc cgtgtagaaa aagcgtctga agttgatgaa
     5521 gccctgcaac gcgccttctc tatcgacggt ccggtgttgg tggatgtggt ggtcgccaaa
     5581 gaagagttag ccattccacc gcagatcaaa ctcgaacagg ccaaaggttt tagcctgtat
     5641 atgttgcgcg caattatcag cggacgcggc gatgaagtga tcgaactggc gaaaacgaac
     5701 tggctaaggt aaaaagggtg gcatttcccg tcataataag gacatgccat gattgattta
     5761 cgcagtgata ccgttacccg accgagccgc gccatgctcg aagcgatgat ggccgccccg
     5821 gttggggacg acgtttacgg agacgaccct accgttaatg ctctgcagga ctatgcagca
     5881 gagctttccg gtaaagaagc cgccattttt ctgcctaccg gcactcaggc caacctggtc
     5941 gctctgctca gtcactgcga acgtggcgaa gagtatattg tcggtcaggc cgcgcataac
     6001 tatctgtttg aagccggtgg cgcagcggtg ctgggcagta ttcagccgca acccatcgac
     6061 gcggctgccg acggcacgct accgctggat aaagtggcga tgaaaatcaa acccgacgat
     6121 atccatttcg cccgcaccaa attactcagt ctggaaaaca cccacaacgg caaagtgctg
     6181 ccgcgtgaat acctgaaaga agcatgggaa tttacccgcg agcgcaatct ggcgctgcat
     6241 gttgacggtg cgcgcatctt taatgccgtg gtggcttacg gctgcgaact gaaagagatc
     6301 acgcaatatt gtgattcgtt caccatttgc ctgtcgaaag gtcttgggac gccagtcggt
     6361 tcattactcg tcggtaatcg tgattacatt aaacgtgcca tccgctggcg gaaaatggcc
     6421 ggtggcggga tgcgtcagtc cgggattctg gctgccgccg ggatgtatgc cctgaaaaat
     6481 aacgtcgcac ggttgcagga agatcacgac aacgccgcct ggatggcgga gcaactgcgt
     6541 gaagcaggcg cggatgtgat gcgtcaggac actaatatgc tgtttgttcg cgtcggggaa
     6601 gaaaatgctg ccgcgttagg cgaatacatg aaagcgagaa acgtgctgat taacgcctcg
     6661 ccgattgtcc gcctggtgac gcatcttgac gtctcgcgcg aacaactggc ggaagtcgcc
     6721 gcccactggc gcgcattcct ggcgcgttaa ggagagagac gtgccgcaac gcattttagt
     6781 tctcggtgcc agtggctaca ttggtcagca tctggtgcgc acactcagcc agcaagggca
     6841 tcagatcctg gcggcggcac gtcatgtcga caggcttgca aagctgcaac tggcaaacgt
     6901 cagttgccat aaagtcgatc tcagctggcc ggataacctt ccggccctgt tgcaggatat
     6961 cgataccgtc tattttctgg tgcacagcat gggcgaaggc ggcgatttta tcgctcagga
     7021 gcgccaggtg gctctcaacg tccgcgatgc gctacgtgaa gtaccagtta agcaattaat
     7081 ctttctcagt tcgttgcagg ccccgccaca tgagcagtcg gatcatctgc gtgctcgtca
     7141 ggctacggcg gacattcttc gtgaagcgaa tgtacctgtg accgaacttc gggccggaat
     7201 tatcgttggc gcaggttcag cggcgttcga agtcatgcgc gatatggtct acaacctgcc
     7261 agtgttaacg ccgccacgct gggtacgttc acgcaccacg cccatcgcgc tggaaaactt
     7321 gctgcactat ctggtggcgt tgttagatca tccggccagc gaacaccgca tcttcgaagc
     7381 cgccggacca gaggtgctca gttatcagca acagtttgaa cattttatgg cggtgagcgg
     7441 taagcgccgc tggttgatcc ccatccccct ccccacccgc tggatttcgg tgtggtttct
     7501 caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg ggctgaaaca
     7561 cgatctgctg gcggatgata ccgcgctacg tgcactcatc ccacaacggc tgatcgcttt
     7621 cgatgacgcg gtacgtagca cgttgaaaga ggaggaaaaa ctggtcaact ccagcgactg
     7681 gggctacgac gctcaggcct ttgcccgctg gcgaccggag tacggttatt ttgccaaaca
     7741 ggcggggttt accgttaaaa cgtccgccag ccttgctgct ttatggcagg tagtgaacca
     7801 aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac gggcgttgat
     7861 ggaccgcgcg atcggtcata agctggcgaa aggccgcccg gagcgcgaat atttacagac
     7921 tggcgatgcg gtagatagct ggaaagtgat tgtcgttgaa ccggaaaaac aacttacgtt
     7981 gttatttggc atgaaagcgc cggggctggg acgactgtgt tttagcctgg aagataaagg
     8041 cgactatcgt actatcgatg tccgcgcttt ctggcatccg cacggtatgc cggggctgtt
     8101 ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg caaaacaaat
     8161 cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat tctttcatgt
     8221 ttcccattgc atcatggggg aaatcacgga agaatgcgca cgttattttc tcaacaacag
     8281 tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg aaacgcggta
     8341 gagtttttat gccagaaagg catcagcgtg cgagcgaccg gtcgcaacga ggcaatgggc
     8401 aaattgctgg aaaaaatggg cgcagagttt gttccggcgg atctgaccga gctggtttca
     8461 tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc cagctttacc
     8521 tcaccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac tcgccgcctg
     8581 ggtgaatggg ctgtcgcctg gggtgtgcgt aactttattc atatctcttc cccctccctg
     8641 tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc
     8701 aacgagtttg cccgcagcaa agcagccagc gaagaagtga tcaatatgct ttcgcaggcg
     8761 aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc gcacgataaa
     8821 gtctttattc cccgtctggc gcatatgatg caccactacg gcagtattct gttaccgcat
     8881 ggcggcagtg cgctggtcga tatgacctac tatgaaaatg ccgtgcacgc aatgtggctg
     8941 gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat caccaacggc
     9001 gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa tattgactgt
     9061 cgtattcgtt ccgtccccta cccgatgctg gatatgatcg cccgcagcat ggagcgttta
     9121 ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa gttgaatttt
     9181 gactttacgc tggatattac gcgtgcgcag gaagagttag gttatcagcc ggtcatcacc
     9241 ctggatgaag gtatcgagaa aactgccgcc tggctacgcg accacggaaa actgccgcgc
     9301 taatcctgcc cgtatttctc cagcaatgct tcggcaatcg cctgagtttc tgcatccgct
     9361 tcgccgttat ataacgtcgg gcggaaatgc atctggaatg ccataatcac gcgccgctgc
     9421 tcgcgcggtg tcatatcagg tttaacgtca taaccgtagc gcgccaaaag ctccagcaat
     9481 gacacggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt aacccgctgc
     9541 gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa taatggcccc
     9601 ggatcgtctt tgcgctgcgg tgcgatatcc gcatgtgcca ctacgttttc cggcttgatg
     9661 tgataacggg caataatatc tttcgccagt ggaataagcg cctgaatctg tgccggttca
     9721 aacggggcaa aatatttcac tccggccgat ttttgccagc cacggttttc cagctcaatg
     9781 ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag
     9841 gccagttctt gttccggcac cagttgccag atgcgcggtt taccgttgta tcgcggtggt
     9901 accgcaggga ccagataatg cgagctgacc tgcttatcgg tcagtgtcgc cagcgagcta
     9961 tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata cgccgcctgc
    10021 gcctggcgtc gggtatcaag ctgatatccc tctttctcga caatgccttt ttcgcctgca
    10081 caccctgcca ataacagagc ggcagcgacc agccagaaga ctcttctcat cgacgcgttt
    10141 tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg accgtttcgt
    10201 agtcgatatc aataccgacg acggcatcgg cccccagcgc ccgcgcctgg gagcctaatt
    10261 cctcaaaggc gatctcccgt gctttacgca gttctttttc atacgcaccg gaacgtccgc
    10321 caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct aaaatcgctt
    10381 cgccggtcac cacaccgcaa tattcaacga tggtctggcc ttccagagtt ggggttgtag
    10441 aaaattgcat gatcgatctc cttgtttgct aagccttcga tctcaaaagc attatcagac
    10501 tgatacgcta ttattgaaag gatatcatta ttatcgtaga acgctttcag agcgatcgct
    10561 tataaggaaa tcattatgcg ctacagcaaa ttgacaatgc ttatcccctg cgcactgctc
    10621 ctcagtgcct gcaccacagt cactccagct tataaagata acggcacacg cagtggtcct
    10681 tgcgttgaag gaggcccgga taacgtggcc cagcaatttt atgactaccg cattttgcac
    10741 cgcagcaacg acatcaccgc cttgcgccct tatctgagcg acaaactggc gacactgctt
    10801 agcgatgcct cccgggataa taaccatcgg gaactactga ccaacgatcc attctccagt
    10861 cgcacaacct tgccagagag cgcccgtgtt gccagtgcct caacaatccc gaatcgtgat
    10921 gcgcgaaata ttcctctgcg tgtggatcta aaaaagggtg atcagagctg gcaggatgaa
    10981 gtgttaatga ttcaggaagg ccagtgttgg gtgatcgacg atgtacgcta tttaggcggc
    11041 agcgttcatg ccacggcggg tacgctccgt cagtccatcg agaatcgcta acgccttgat
    11101 caaccaataa accccgtaaa ttgtccggca tttcgtgagg aatgccgaca tttatgctcg
    11161 ccgaccaccg cccccgttat tttgtgctat gtttattgaa taatgcgctt tgcttttaac
    11221 ttttaaagca gaaatattgc ataattattc tgtcaaaggt actatcttcg acctcaatag
    11281 ctatcagact gccagtatac gaatgtcaat gagtattcaa ttaaacggca ttaattgctt
    11341 ctacggcgcg catcaggcgc tgttcgatat cacgctggat tgcccacagg gcgaaacgct
    11401 ggtgttactt ggccccagcg gcgcgggtaa aagctcgctg ctgcgtgtac tcaatctgct
    11461 tgagatgccg cgctccggta cgctcaacat tgcaggcaac catttcgatt tcaccaaaac
    11521 accctctgac aaagcgattc gcgatttgcg tcgtaacgtt ggcatggtgt ttcagcaata
    11581 caacctgtgg ccgcatctga ccgtgcagca aaacctgatt gaagcgccct gccgtgtact
    11641 ggggttgagt aaagatcagg cgctggcccg tgcagaaaaa ctgctggaac gtctgcgtct
    11701 caaaccttat agcgatcgtt acccgctgca tctttctggt ggtcagcagc agcgtgttgc
    11761 tattgcccgt gcgttgatga tggaaccgca ggtactgctg ttcgatgaac cgaccgccgc
    11821 actggacccg gaaattacgg cacaaatcgt cagcatcatt cgtgagctgg cagaaacgaa
    11881 tattacccag gtgatcgtca cccacgaagt tgaagtggcg cgtaaaaccg ccagccgagt
    11941 ggtgtatatg gaaaatggtc atatcgtaga acaaggcgac gcgagctgct ttaccgagcc
    12001 gcaaaccgaa gcatttaaaa actatctctc tcactaagat tcgggataac aacaatgaaa
    12061 aaagttctga ttgccgcgtt aattgcaggt tttagtcttt ccgccacagc tgccgaaacc
    12121 attcgttttg ctaccgaagc ctcctatccg ccgtttgaat cgattgatgc aaacaaccag
    12181 atcgttggtt ttgacgtcga cctggcacag gcgctgtgta aagagattga tgcaacctgt
    12241 actttctcta accaggcgtt tgacagcctg atcccaagcc tgaaattccg tcgcgtagaa
    12301 gcagtgatgg cgggcatgga tatcactccg gagcgtgaaa agcaggtact gtttaccacc
    12361 ccgtactatg acaactctgc cctgtttgtg ggtcagcaag gcaaatacac cagtgttgat
    12421 cagctgaaag gcaaaaaagt cggcgtacag aacgggacga cacaccagaa attcattatg
    12481 gataagcacc cggaaatcac taccgttccg tatgacagct accagaacgc aaaactggat
    12541 ctgcaaaacg ggcgtatcga cggcgtcttc ggtgacaccg cagtggtcac tgagtggctg
    12601 aaagataacc cgaaactggc ggcggtgggc gacaaagtga ccgataaaga ttacttcggc
    12661 actggcctcg gcatcgcggt acgtcagggc aacactgagc tgcagcagaa actcaacact
    12721 gcgctggaaa aagtgaagaa agatggcact tacgaaacca tctacaacaa atggttccag
    12781 aagtaattcc tgatgaatga attttttcct ttagcaagcg ccgccgggat gaccgtcggc
    12841 cttgccgttt gtgcattgat tgtcgggctg gcgctggcga tgttctttgc ggtatgggag
    12901 tcggcaaaat ggcgtcctgt cgcgtgggca ggttcagcgc tggtaaccat tctgcgtggc
    12961 ctgccagaaa ttctggtggt gctgtttatc tattttggct cctcgcagct gctgctgacg
    13021 ctttcggatg gcttcactat caatcttggg ttcgtgcaga tcccagtgca gatggacatt
    13081 gagaacttcg acgttagccc gttcctttgt ggtgtcatcg ctctgtcact gctgtatgcc
    13141 gcctatgcct cgcaaacgct tcggggcgcg ttgaaagcgg tgccggtggg tcagtgggag
    13201 tctggtcagg cgctggggct gtcgaaatcg gctatctttt tccgtctggt gatgccgcag
    13261 atgtggcgtc atgcgctgcc tggcctcggt aaccagtggc tggtgctgct gaaagatacc
    13321 gcgctggtca gtttgattag tgtgaatgat ttaatgctgc aaacaaaaag catcgctact
    13381 cgtacccagg aaccatttac ctggtacatt gtggcggcgg cgatttacct ggtgatcacc
    13441 ctgctcagtc agtacattct caaacgcatt gacctgcgcg cgacacgttt tgagcggagg
    13501 cccagctaat gtttgagtat ttacccgaac tgatgaaagg gctacacacc agcctgacgc
    13561 taaccgttgc ctcgctgatt gtggcactga ttctggcatt gatttttacc atcatcctga
    13621 cgctgaaaac gccggtgctg gtgtggctgg tgcggggtta tatcacgctg tttaccggta
    13681 cgccgctgct ggtgcagatc ttcctgattt attacgggcc gggccagttt ccgactttgc
    13741 aggagtatcc ggcactgtgg catttgttgt cagaaccgtg gttatgtgcg ctgattgcgt
    13801 tatcgctgaa cagtgcggcg tataccacgc agctgtttta cggtgcaatt cgtgcgatcc
    13861 cagaaggtca gtggcagtcc tgtagcgccc tgggaatgag caaaaaagat acgctggcga
    13921 tcctgctgcc gtatgccttt aaacgctcgc tctcttctta ttccaacgaa gtggtgctgg
    13981 tgttcaaaag tacctctctg gcttacacca ttacgctgat ggaagtgatg ggatacagcc
    14041 agttgttgta cggacgcacc tacgatgtaa tggtgttcgg tgcggcaggg attatttacc
    14101 tggtcgtcaa cggcctgctg acgctgatga tgcgtctgat cgagcgcaaa gcactggcat
    14161 tcgaacggcg aaattaatgt agctgcgtac attcgccggg ggggtttgta ggtctgataa
    14221 gacgcgccag cgtcgcatca gacatcagca cggtgcctgt gccggatgcg gcgtggacgc
    14281 cttatccggc ctacatagtg cataaattct tataatgaag acggacaact cactaaggta
    14341 tccgtctttt tttatttcat ttaaattatt taatcatatt tattgcatat aaattcactt
    14401 gatggcattg ttatcccatg ccgcagacac ggccaaaaat cataagattg acagacggga
    14461 gttccatcat gaaaaagtta gttcttgccg ctttacttgc ttcctttact ttcggtgctt
    14521 ctgccgcaga gaaaatcaat tttggcgttt cagccaccta tccacccttt gaatctatag
    14581 gtgctaataa tgagattgtc ggctttgata tcgatctggc aaaagccttg tgcaaacaaa
    14641 tgcaggcaga atgtactttt actaatcacg cgttcgacag cctgatcccg tcactgaaat
    14701 tcagaaaata tgacgccgta atctccggta tggatatcac gcccgagcgt agcaagcagg
    14761 tatcgtttac cacgccctac tatgaaaact cagccgtcgt gattgccaaa aaagatacct
    14821 acaaaacgtt tgccgatctg aaaggcaaac gtattgggat ggaaaacggc accacgcacc
    14881 agaaatatat tcaggatcag caccccgaag tgaaaactgt ctcctatgac agttatcaga
    14941 atgcctttat cgatctgaaa aatggtcgta ttgatggggt atttggtgac acagcggtgg
    15001 taaacgaatg gctgaaaacc aatccgcaac tgggcgttgc tactgaaaaa gtgaccgatc
    15061 cgcaatactt cggcaccggt ctgggcatcg ctgtacgtcc ggataacaaa gctctgctgg
    15121 aaaaactgaa taacgcgctg gcagcaatta aagctgacgg tacatatcag aaaatcaatg
    15181 accagtggtt tccgcagtaa ttgagactga tgacaaaagt aagattgcct gatgcgctgc
    15241 gcttatccgg cccacatcaa ctctgcaatg tattgattta gcatgatttt gtaggccgga
    15301 taaggcgtac acgccgcatc cggcatgaac aaagcgcacc tgatcagcaa tctgaagccg
    15361 cacctgcggc ttttttattg cttcaccagc agcgtcagca cttcatagtg cgcggtgtgc
    15421 gggaacatat caaaaagctg tacccgttcg atgcggtaac ctggcagttc gcggatatct
    15481 ttcgccatgg tttgggcgtt acagctggag tagataataa aacgcggtgc cattgttgag
    15541 agataatcac atagcggttt accaatgccg cggcgcggcg gattaaccag caccagctcc
    15601 ggcacctccc cctgagcggt ggcaaactga gtggagtcca gcgcctgaaa ttgcaaacgc
    15661 gttaagccca gttcagcggc tgactgcttt gcacaggcaa tggcctctga tgcaatttcg
    15721 atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac caaagccacc cacaccgcag
    15781 aacagatccc acatatgttt aactggcagc tgccgtaccc agtcgcgcgc ggtagcgtac
    15841 agctggctgg cgaccgccgg attagtctgg aagaaacttt gcggacggat ccacagcggt
    15901 acgtcattaa aacgctctgc cagcgcctgt tgttcagtaa ggtagatctc cgtctcccct
    15961 tccataatcg ccatatgtac cggctgaata ttgacggtaa taactttcag ttgcggtagt
    16021 tgttcctgta accacggcag cgccttacgc agttgtgcca gtttggtttc agaacgcagt
    16081 acaaagcgca gcatcatgcc gccatcgctc tggctttcag tcagcagaat gtatttcagt
    16141 tcgccacgct tacgcgccac gttgtagggt gtcagccccg cacgggcgat aaacggtttt
    16201 agcgccgcaa aaacgggcgc aaatgaggca ggataaagcg ggcagtcaca aaggtcttct
    16261 ggtgtgccgt cgcgatgcag cataccgagc agtggttttt caacgctacc actcaccacc
    16321 attttggctt tattacgaaa cccttgttcc gggcctgaca ccggcgcgca ccattcctca
    16381 accggaaaat cggcgagcag atttttaaga tcggcggttt tagcggagag ttgctctgga
    16441 atcggctgcg ttatccactg acaggaacga cagcgacccg cgtcgtaaag tgcgcactgc
    16501 atacattgac cttcacatca tcaggggcga cgattataca cattattgca actggaagaa
    16561 ccgccgactg gtcgagggaa cgaacagcag catcaggatg agcatatccg gcagcttctg
    16621 cagcatcagg ctatggaaga tttcacgttt tgattcaccg ggaatgctga acagctccgg
    16681 ataaccgtac cccagcgaag ccgcccacaa gtaacttgcg gcggtgattt gtgtcagcag
    16741 atatagccag cgcgcccagc gacgcccttt caccagtgaa aacgcacacc agatctcaat
    16801 gaaaaccagc accagactgc ttaaaaagac cagcgttaaa ctccaggttt gtacgctgcg
    16861 atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg aaaatcatca gcacgtcgag
    16921 gccacggatc ataataatgg cgagcgccgc tacctgcacc agcgcaggca cattcaggcg
    16981 agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt tcgtcttcca tgaaaacaat
    17041 gccgcggcat gcgcggcatt atgtagccag gttggcaaat tttagtgtct tcagccatgt
    17101 cttgcacgct ggatatcgcg tatccgctgt ttttctgcgc gagccatcag ataccaggcg
    17161 ataaatccga caattccgac cgcaccgaga attaacgttg caagggcgtt gatttccgga
    17221 ttcaccccca tccgcacgct ggaaaagacc agcatcggta acgtggtggc tcccggcccg
    17281 gaaacaaagc tggcgatcac cagatcatca agcgacaaag taaaagccag taaccagcca
    17341 gaaatgatcg cgggcatgat catcggtagc gtaatgacga aaaacacttt cagcggcgtc
    17401 gcaccgagat ccatcgcggc ttcttctatc gagcgatcca gttcccgcaa tcttgaagaa
    17461 ataacgaccg ccacataagc cgtacaaaaa gtgacatgcg ccagccagat ggtgagcata
    17521 ccgcggtccg caggccagcc aatggcatga gcaagcgcga cgaataataa caacagcgac
    17581 aagcccgtga tgacatctgg cattaccagc ggcgcggtga tcataaaggc aaagccattt
    17641 gatccgcgaa acctgccaaa gcgcaccagc accaccgccg caatagtccc gaggatcgcc
    17701 gccgccgttg ccgcacaggc agcaattgtc aggcttaaac caaccgcact catcatcgcg
    17761 tcatcgcgca ataactcacc ataccagcgc gttgaccagc cggcccacac cgtcaccagc
    17821 ttcgagctgt taaacgaata gataaccagc atcagcattg gcgcgtagag aaaggtaaag
    17881 cccagcagca aaatcacaat ccgccagggt gaacgaacta ccggtaaatt attcatccgt
    17941 gttctcccac gcttttttgc tggtgtttgt ggaaccacat tatcggcaca attagcagca
    18001 gcaacatgat gatcgctacc gccgaggcca ccggccagtc gcggttattg aaaaactctt
    18061 gccatagcac gcgcccgatc atgatgctgt ccgggccacc gagcagttcc gggatcacaa
    18121 actcgcccac agccgggata aacaccagca tcgatccggc aataatccca cctttagtca
    18181 gcggcacgat cacagtaaag aacgttttca gcggtcgtgc accgagatcc agcgctgctt
    18241 ccaccagcga ataatcaata cgaatcaacg cggtataaat cggcagtacc ataaacggca
    18301 cgtaggcgta aacaatgcca atataaactg ccagattggt atgaagaatg gtcagcggtt
    18361 gatcgataac ccccagccac agcagaaaat tattcagcac accgttgttt tttaatattc
    18421 ccatccaggc ataaacgcgg atcagaaacg aggtccacga cggcaggatc accagtagta
    18481 ataaaatatt acgggtcgaa ggcttactgt gcgccaccgc ccacgccagc ggatagccga
    18541 tcagtaaaca gcaaaatgtc gaaatcgcag ccacctgtag cgactggaga taagcatcga
    18601 aatagagcgg atcgtcggtc agttgcagaa aattaccgag attaagagtg atggacagtt
    18661 gcccgtcagc ccactccatc agttcggtat aaggtggaat agcgcgcgcc atctccgaca
    18721 ggcttatttt aaagacaatc agaaatggca gcagaaacag caagatcaac cagatatatg
    18781 gcaacgcaat gaccagtttg cgcccatgct tcatttgcag ctgcgacaac cacagcttaa
    18841 atccgcccgg cggtttcgac tgggcagcag gttcaagtgt attcatcttt gctccttaaa
    18901 ccgtcagcac cacacagctg tccacttccc agcacaaacg cacttcgtcg ccccaggtcg
    18961 gtaacccttt acgatgacga tgggcgtttt gtagctgggc gctaatcatc tgcccacttt
    19021 tcagacgaac gtgatacacc gaaagatcgc cgagataggc aatgtgtatc acctccccca
    19081 ccgcgaagtt acaaccattg gcgggcggct cttcgcaaag catgattttt tccgggcgca
    19141 gcgctacgtg taccggcacg ttatcgacca ctgaagcatc cgcatcgact ttcagtggat
    19201 gcaccagccc cggcgaatca agcaccaggc catcttcctg acgctctttg agtacacctt
    19261 caaagacatt aaccgaaccg ataaattcag cgctgtagcg ggtagttgga tgctcgtaga
    19321 tctcttccgg ctcgccaatc tggacaaatt tcccacgatt cataatggcg atacgccccg
    19381 ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac acaagtcaca ccgacgcgct
    19441 ccagaatatc caccacttca agctgcatcc ggtcacgcag ctttttatcc agcgcgccca
    19501 tcggctcatc gagcagtaat agtttcgggc gtttggcaag gcttcgggcc agggccacac
    19561 gctgtcgctg accaccggaa agctgatgcg gtttgcgttt ggcgaactcc tgcatgtgca
    19621 ccaggccgag catctcatgg acccgactgg caatttccgc tttcggtagt ttgtcctgtt
    19681 tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg aaacagcgcg taagactgaa
    19741 acatcatatt gatggggcgc aggtaaggcg gcacctgtga caaatcgacg ccatcaagca
    19801 ttatctgtcc ggcagaaggt tgttcgaaac ctgccagcat acgcagcagc gtggacttgc
    19861 cacagccgga tgcgcccagc agcgcgaaga tttcaccttt gtagatggtc aggctgacat
    19921 catccaccgc atgttgacca tcgtaggatt tggtgaggtt gcggatttct aatagcggcg
    19981 tcagcgcctt acgggttttc gcctgcgggc gagggatagc gtcattcacg gctgctctcc
    20041 ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt gccggcgggt gtggtgcgcc
    20101 cgcccggcat ctaaggctgc ggattatttt ccgctcttca ctttggtcca cgcgcgggtg
    20161 cgcacacggt cgattttcgg gtcctgcact ttcagagtga acagcttcgc acgaacatcc
    20221 gcaggcggat aaatacccgg gttctcacgg acttccgcac tcaccagcgg cgtggctgct
    20281 ttattggcgt tggcatagaa cacatggtcg gaaatatgcg ccaccacatc cgggcgcagc
    20341 aggtaattca ggaactgata ggcttcgtct ttatttttgg catcggcagg catggcgaat
    20401 acatcaaaga atgccatcgc cccctctttc ggaatcgaga acgagacatt cacgccattc
    20461 ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcgc ctgcccaacc aatcgctacg
    20521 cagatgtcac cattcgccag gtcattaata tattgcgatg aatggaaata acgaatgttc
    20581 gggcgcagct ttaacagcag atcggtagcc ggtccggtgt aatcatctgc tttagtgctg
    20641 ttgggatctt tgccgagata attcaacacg gtagcaaaaa cttcttctgg cgcatccagg
    20701 aaagagacac cgcagctttt cagtttttcc agattttcag gtttgaggat caagtcccag
    20761 ctatcgacgg gcgcgttttc gcccagcaca gctttaactt tatcaacgtt atagccaatc
    20821 ccggtcgtcg cccacatata gggcatagca aatttattgt cgggatcgtg tttggcgacc
    20881 agcttcagca gctccgggtc gagattcttc cactccggca atttgctttt gtccagcggc
    20941 tggaaaactc ccgcagtcaa ctggcgttca agaaagctgg cagatggaac caccaagtca
    21001 aagccggtgc tacccgccat taatttgcct tccagtactt cgttagagtc gaaaacatcg
    21061 tagacgactt taataccggt ttctttttca aaattggcca ccgtgtccgg ggcgatataa
    21121 tcagaccagt tataaacgtg gagtgttttt tgttcagccg cgagcgtgcc gacagagacg
    21181 gccatcagag cacccgcagc cagacccgaa agccattttt tatttaaggc ggtcatatct
    21241 ctttccttct gaaagttcgt taacaaacga acaaaagttg tcacactaaa agatatgcaa
    21301 taaacgtgca tattttttat cggcggggtg taagaaaaga gaactctctc ccggccagca
    21361 ctgctcgcta tataaaagca tcgcaagaat aactttagcc agggtttgag actatagctc
    21421 ggattaaaaa acggtattta acaaattttt atgcgtattt gctctatgtg ataaagccaa
    21481 atcgacacaa gagaagtgaa ataatcttta agaaaaggtt aaaaacagca gataattttg
    21541 cagcgcgcag ccactgcacc agcgactgcg tgctcgaaag gagttatcag tggaggaaat
    21601 agttttcggt aacgttgttt tgcccttcat cgtcagtcgg taacagcagt tgatgggcat
    21661 tagcttcaag aatgaccatc gaaatctgct cttcgctctg acgcacgaac catgcaaact
    21721 gctcataggt tacgccctgc ataacggata aagactggca aaccaccagc tttggcagat
    21781 tatcatcctg catatcaaga aatgctttca cggtcagcga actggcattg atggcagata
    21841 aatccgccgc cagcggcaat acggctgaag gtcggacttc cgccatggca gaaaacagga
    21901 tcgtgttatc gatcagatcg attttggcat caaagacacc gtcgaaattc tgcatatggg
    21961 gcagatgcag agcctgacag ttatcacatt caaaaaaact catccccagg tcatcgagcc
    22021 attgacgcag cgtatccaga ccaggaacga ccagcgatgt cataattttg tacaacctct
    22081 ttcgataaaa agaccggcac agcttacgca aaaagcgcag gcaaaaccat gatcagtaat
    22141 gtgattgcga ctaaccaccc gttttcaggc aatattcggt cgtagcgtgg cgttcgatcc
    22201 agcggatcat tttacccgcg atgtcgatac cggtggtttt ttctattcct tccagccccg
    22261 gcgacgcatt cacctccatc accaacggcc cgcgattagc acgcagaata tccacaccag
    22321 caacgtccag cgccatcgtt cgcgcggctt ttatcgcgat ttcacgctcc tgtggtgtga
    22381 tactggcgac gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg ccctctttcg
    22441 cccgccgttc aatcgcagcg accacttcat cgccaacaac cagacagcgg atatcgcacc
    22501 cttgcgcctc tttgatatat tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt
    22561 ctatcacgct ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc
    22621 cttcaaccaa cttgaccacc agcggcgcac caccgaccat gtcgattaaa tcgctggtat
    22681 catccggcga atgcgcaatg cccgtgacag gcaggtcgat gccctgacgc gccagcagtt
    22741 gcatggaacg caatttgtca cgcgcccggg caatggcgac cgactcattg agcggatagc
    22801 tccccagcat ctcgaactgg cgcagtgccg ccgtcccata aaaggtaatg gcggtgccaa
    22861 tacgcgggat cactgcgtca aaatggggta acttgcggcc tttgtagtga atagaagacg
    22921 ccgcaggatt tatgttcatg tagcaagaaa gcggatcaag aatttcaacc aggtgaccgc
    22981 gctgtatagc ggcttcacgc agccgcttac acgaatagag cgttccatcc cgggacaata
    23041 tggcaatttt caccctgcac ctctctgtca gacctggtca aaagcccgcg tatcatacat
    23101 cgacgtggcg gttttagcgc gtcgcccaac cctgtttgtg caaataatcc agaataaatg
    23161 ggcggctttc tttaatgatt gttcggcgga tatgatcgct ccaggtatcc cggcgattat
    23221 tgctgccacg ggtgaggtaa tattccgcca gttgctcgtc atactgcgcc agtgcgcctt
    23281 tatccagcgg ttgatagctg ttttcatgca ccaaaatgga ggccggtaaa cgcggcttaa
    23341 gatccggatt atccgcaggc cagccaaggc acagcccaaa cagcggcaga acatgctgcg
    23401 gtaatttaag cagtttcgtc accgcttcaa tattattgcg caggccgccg atatataccc
    23461 cgcccaatcc cagcgattcc gctgcgatta atgcattctg cgccatcatt gccgtatcaa
    23521 cgacaccgag caacagttgt tccgccaggc cgagctgagc atccggacag atctgtaaat
    23581 ggcggttaaa gtcggcacag aacacccaga actccgccgc ttgcgctacg tgtttttgcc
    23641 cgccggtcag cgtcaccagt tcttcacgta acgctttgtc ggtaatgcga ataatgctac
    23701 tgcactgcaa aaaactggaa ctggacgtcg cacgggcgct gttaataatc gcctcacgct
    23761 gcgcttcgga aatgggttca tcagtgaaat ggcgaatgga gcgatggcca caaataagtt
    23821 caatggttgg cgtcattatc tttttctctt tctgaacgtg aatattgcgg tggacggttc
    23881 atcagctgtg gggcaagacg ttttgccacc tgaagaataa ccaccaccgc agcgggaagc
    23941 atgagcaaaa caccgagaaa aatcatcaga atctgcactt ctggccgaga aaatggctca
    24001 ggcagcgaca gggagtcgct taccgacagc agcgccaccg ccagtagcat cattccgata
    24061 aattccagta tcaacacgcc tttaggcaat ttaccgatcg cgcgcatacg cttccctctg
    24121 caaagtgagc cttcagtcta aaacttttca ctgtattgtg tttaacagtt atagctttta
    24181 gcaattaatg caacaggtta aacctacttt cagcgaatac attttagcgt gatcattaca
    24241 ggcataaatc tatgaggaga gaaataatgc aaaccgttat ttttggtcgt tcgggttgcc
    24301 cttactgtgt gcgtgcaaaa gatctggctg agaaattgag caatgaacgc gatgattttc
    24361 agtatcagta tgtagatatt cgtgcggaag ggatcactaa agaagatcta caacaaaagg
    24421 caggtaaacc cgtagaaacc gtgccgcaga tttttgtcga tcagcaacat atcggcggct
    24481 ataccgattt tgctgcatgg gtgaaagaaa atctggacgc ctgatcgtct gacaagccct
    24541 cgcgttgagg gctttactga ttttttctgt gctgtggttt aaacaaacta ctgataaata
    24601 agaaacacag tgcccccagc gcacaccaga acaccgcgct tagtaaccat gccagctctt
    24661 gccagaatga gcgcgtcggt gaaaaaaaca gccgcataat gagcatcgaa cagggtgccg
    24721 ccagcattgc gccaaacaga ggtttcagga cttctctacg ctgtgaaaag aagctggcga
    24781 ctgctccagg aagaatgaaa aatagcaagc cgatttcagg atgcccggca gcccgaaaag
    24841 cgcctttcat gtgcgtcgcc agaaaaaggc acaccacaat gaagaggaca aaacagcaga
    24901 ttgcccccgc ccaacgttgt ttatgtttca ctcgttcctc ctgacactgc gtctatcgaa
    24961 cacatttttc gccagtgtgg cgttcagtaa gataaagccg cttcgcattc catgctaata
    25021 taggccaacg caattcatat agccgttgat acctaatgtg attacactag taaaatatat
    25081 tgttacttta ctatcgttta ggtgcgctga atgaatctgc gccctgaatt ctggtaaaaa
    25141 acattatcgt aaattaccat ttctttcaac agcttactag taaacaagaa gttagcctcc
    25201 gtgaatataa acgtcgccga attgttaaat gggaattaca ttctgttatt atttgtggtc
    25261 ctcgcgcttg ggctatgtct cggaaagtta cgacttggtt cgatccaact gggtaattcc
    25321 attggcgttt tagtcgtatc gctgttatta ggccaacaac atttcagcat taacaccgat
    25381 gcgcttaatc ttggctttat gctgtttatt ttctgcgtcg gggtcgaagc cggaccgaac
    25441 tttttttcca ttttttttcg cgatgggaaa aattacctaa tgttagcact ggtgatggtt
    25501 ggcagtgcgc tggtgatcgc cttagggtta ggtaagctgt ttggctggga tattggcctg
    25561 acggccggta tgttagcagg ctctatgacg tcgacaccgg ttctggtcgg tgctggcgat
    25621 acactgcgtc attccggcat ggaaagcagg cagctctcac tggcactgga taatctgagc
    25681 ctcgggtatg ccttaaccta tttaatcggt ctggtgagtt tgattgttgg tgcgcgttac
    25741 ttgccgaaat tgcagcatca ggacttacag accagcgccc agcaaatcgc ccgcgaacgt
    25801 ggcctggaca ctgatgccaa ccgtaaggtt tatttaccgg tgatccgcgc ctatcgcgtc
    25861 ggcccggaac tggtggcctg gaccgacggc aaaaatctgc gtgaactggg tatttatcga
    25921 caaaccggct gctacattga acgtattcga cgtaacggga ttctggcaaa tccagacggt
    25981 gatgccgtgc tacaaatggg cgatgaaata gcgttggtag gctatcccga cgcccatgcc
    26041 cgactcgatc ccagcttccg taacggtaaa gaagttttcg atcgtgacct tctcgacatg
    26101 cgtatcgtca ctgaagaagt ggtcgttaaa aaccataacg ctgtaggtaa acgtctcgca
    26161 caactgaagt tgaccgatca cggttgcttc cttaaccgcg tcattcgtag ccagattgag
    26221 atgccgatag atgacaacgt cgtgcttaac aaaggtgacg ttttacaagt cagcggcgat
    26281 gcccgccgcg taaaaaccat cgccgatcgc atcggcttta tctcgattca cagccaggtc
    26341 actgacctgc tggcattctg cgccttcttt gttattgggc tgatgatcgg gatgatcacc
    26401 ttccagttca gcacattcag tttcggcatg gggaacgctg ccgggttgtt attcgccgga
    26461 attatgctgg gctttatgcg tgctaaccac ccgaccttcg gttacattcc gcaaggtgca
    26521 ttaagcatgg tgaaagagtt cggcttgatg gtgtttatgg caggcgttgg tctgagcgcc
    26581 ggtagcggta ttaataacgg cctgggcgcg attggcggtc agatgttgat tgccggatta
    26641 attgtcagtc ttgtgcccgt ggttatctgt ttcttgttcg gtgcttatgt attgcgaatg
    26701 aaccgcgcac tgttgttcgg cgcaatgatg ggcgcacgca cctgcgcgcc ggcaatggag
    26761 atcatcagtg atacagctcg cagtaacatc cctgcgctgg gctatgcggg cacctacgca
    26821 atcgccaacg tcctgctgac gctggcaggg acaatcatcg tcatggtatg gccaggatta
    26881 ggataaaact gaagttgccc tgaaaatgaa atttttttgc acaaccgcag aacttttccg
    26941 cagggcatca gtcttaatta gtgccactgc ttttctttga tgtccccatt ttgtggagcc
    27001 catcaacccc gccatttcgg ttcaaggttg atgggttttt tgttatctaa aatttatcta
    27061 ctaccctgca accctctcaa ccatcctcaa aatctcctcg cgcgatagcg gcttacggtc
    27121 ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca agcgcgcggg cggttccggg
    27181 ttcaaaccat tgttcgagcg tttgctgact gcgctgcatc cagttttgca ttaccgtttt
    27241 taatagcggt tttcggctag ccagcgcgta gagctggtac atcagctcca tgttatccgg
    27301 cgttgcaacc tgtgagctgt agatcatatc ggtgatagcc tggcatgcgc cttgagcatc
    27361 actaacatcg ctaaaaaatg cctgatattg ccgggacata atctcagtaa aactgctgaa
    27421 cgcctccagc aacaactcat caattcctga aaaatagtag gtcatcgatc ccaacggtac
    27481 cccggcaagg gtagcaattt tgcggtgcgt aacagcatgt attccgtaaa gtttcaccgc
    27541 ctccagcgtg gcctggataa ttttttctcg ccgttgcgga tcgttagcgc gacgcatgtc
    27601 atttcctctg tctgcaattg tgtacaaatg tacacaacct tgctaatgtt gtcttccctc
    27661 ttcttatttt gacgtgatct atgaccgtaa attcttcacg taatgcattg aaacgccgaa
    27721 cttgggcgct gtttatgttc ttctttttgc caggcctgtt aatggcgtcc tgggcaaccc
    27781 gtacgcctgc tatccgcgat attctctctg tctcgatcgc tgaaatgggc ggtgttctct
    27841 ttggtctgtc gatcggttcg atgagcggta ttctctgctc ggcgtggtta gtgaaacgct
    27901 ttgggacacg taatgtcatc ctggtcacga tgtcctgcgc attgatcggg atgatgatat
    27961 taagtctggc actctggctg acatcgcccc tgctctttgc cgttggtctc ggcgtctttg
    28021 gggcaagttt tggttctgcg gaagtggcga taaacgttga aggtgccgcc gttgagcgag
    28081 aaatgaataa aacggttttg ccgatgatgc acggttttta tagcctgggc acgctggcag
    28141 gcgctggtgt cgggatggca ctgacggcct ttggcgttcc ggcaacggtg cacatttcat
    28201 tggcggcgct ggtaggtatc gcgcctattt atatcgccat tcaggcaatc cctgacggta
    28261 cgggcaaaaa tgctgccgat ggcacccagc atggcgaaaa aggcattcct ttttatcgcg
    28321 atatccagtt gctgttgatt ggtgttgtag tgctggcgat ggcctttgcc gaaggttctg
    28381 ccaacgactg gttaccctta ttaatggttg acggtcacgg ttttagccct acttccggct
    28441 cgctgattta tgccggtttt accctgggga tgaccgttgg acgcttcacc ggtggttggt
    28501 tcatcgaccg ttacagtcgc gttgccgtgg ttcgggccag tgcactaatg ggggcgttgg
    28561 gtattgggct gattattttt gtcgatagcg cctgggtcgc aggagtgtct gttgtacttt
    28621 ggggactggg tgcctcgctg ggcttcccgc tgaccatttc tgccgccagc gataccggcc
    28681 ccgatgcacc aacccgcgtc agtgtggtag caacgaccgg ttatctggct ttcctcgtcg
    28741 ggccgccgct gctgggctat ctcggcgaac attatggatt acgtagtgca atgctggttg
    28801 tactggcgct ggttattctc gcggctattg tcgcgaaagc cgtcgccaaa cccgatacca
    28861 aaacgcagac ggcgatggag aatagttgat gagcattaaa ttaattgcgg tagacatgga
    28921 tggtactttc ttaagcgatc aaaaaaccta taaccgtgag cggtttatgg ctcagtatca
    28981 gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc gggaatcaat attatcagtt
    29041 gatctctttc ttccctgaaa ttgcgaatga aattgccttt gtggctgaaa acggcggctg
    29101 ggtagtgagt gaaggcaaag atgtttttaa tggcgagctg tcgaaggatg cctttactac
    29161 tgtcgtggaa catttgctga cgcgcccgga agtggaaatt attgcctgcg gaaaaaatag
    29221 cgcctatacg ctcaaaaaat atgacgatgc catgaaaacg gtggcggaaa tgtattatca
    29281 ccgtctggaa tacgtcgata actttgacaa cttagaagat attttcttta agtttggcct
    29341 gaatctttcc gatgaactga ttccacaagt acaaaaagca ttacatgagg ccatcggcga
    29401 tattatggtg ccagtccaca ccggcaacgg cagcatcgat ctgattatcc ccggcgtaca
    29461 taaagccaat ggccttcgcc aactgcagaa attatgggga atagacgaca gcgaagtggt
    29521 ggtctttggc gatggcggta acgatattga gatgctgcgt caggcaggct ttagttttgc
    29581 aatggaaaat gccggcagcg cggtcgtcgc agcggcaaaa taccgggcag gctccaataa
    29641 ccgtgaaggc gtactggatg tgatcgataa agtgcttaaa cacgaagcgc catttgacca
    29701 ataaaaacgg cagcacgccc ggcaacacaa tacctgaatg aaggctgcat aattcgcgga
    29761 aattaacccg ctgcattatt tcaggcgaaa tgattatgaa aaattgtcta ctgttgggcg
    29821 cgcttttaat gggctttact ggtgtggcga tggcgcaaag tgtcaccgtc gatgtgccga
    29881 gtggatacaa agtggttgta gttcctgatt cagtcagcgt tccacaagcg gtcagcgtcg
    29941 cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc tccggcttac cgcccgcatc
    30001 cttatgctcg ccatcttgcc agcgtaggtg aagggatggt tatcgaacac cagattgacg
    30061 accatcacca ttaattcgag aatgcctgat cgcacaaagc agtcaggcat ttttttaccc
    30121 ttcgtgagaa tttcccatct gtttatcttt taaaaagata accatcagcg acagccacaa
    30181 aattccgttg acaaggttga agagattaaa ctgtccgttg cccccgttca gccaggcatg
    30241 tttgctgatt tcaataccaa cggtaaagat cagcatttgc agcattccca tcgccgcaga
    30301 aaccgtacct ttactcatat cgctggcaaa cagggttaat cgcaccagtc ccgcattcgc
    30361 cagaccaata ccgaaagcat aaatacttaa cccggcagtc atccataaat acgcgtgcga
    30421 tgagataacc gttgccgcag cagcgaccaa cagaccaatc ataatcggcc agccgcccat
    30481 aattatcagc gaacgtacgg tgcggcgcga ggtcaaacgc gctaacaaca agttacccgc
    30541 aattaacgcc ccgaaaatag gcacttgcag caagccatat tcatagctgc tcaactgctc
    30601 gccggtaatg atgataatcg gcgactgggc gatccacgcc agcaatggca ggctaacgaa
    30661 tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc agcaccagct tatagtcacg
    30721 accgagttct ttcagtgaca gtttctcgcc tatacgcgtg gcggtttcag gcatagctcg
    30781 ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac aaaacaaaca tcccctccca
    30841 gggcagcaca tggatccacg ccgcgcccac cagcggacca agtagcggag caatcagcgc
    30901 cacgttcgcc atcagcgcgg tgatcttgat acaaaccgcc tcttcgaagg attcctgaat
    30961 tgcggcgtat cccacagcgc caatgaaaca gaggcttatg ccctgcaaga agcgcaacag
    31021 ggtgaattgt tcaatgtttt gcgccagcaa tattgccaga caggtgatga taaaccacac
    31081 cacccccgcc agcatcaccg gacggcgacc aatacgatcc gacagcggcc ccaggagcca
    31141 ttgtaaaaac atcccgcccg ccagatatgc ggtcattgaa gtaggaaccc aatcaatgcc
    31201 cgcctgatat tgttccacca cggccaacat accgggttga atcatatcgt tgccgatata
    31261 ggttgaaaat tcgtaaagca ccagacagag agggaaaagt aacgcctgac gtccaagcct
    31321 ggcaccggaa gctaatttat tttgcatgca atttcttcgc caataataat cgcgcagagt
    31381 ttaataaaag cacagctaac gagaaagcga attttgtagc tgaaaccacg gttaagcaca
    31441 ttcttacatt attgcgagta tagctacgct ttctttaagt tttatttaac ctatgcccgt
    31501 tacaattacc caccgtaaac aggccgcttg agggaaataa gacgatgccg ctttacccag
    31561 tttaacctgc actttattct caacgacttg cctgtattgg ctccctttta atcactttgc
    31621 gtcgggaagt tatgctggaa aatttgaatc tctctctatt ctctcttatt aacgcgacgc
    31681 cagactcggc tccgtggatg atctcgttgg cgatttttat tgctaaagat ttgattaccg
    31741 tggtaccgct gctggccgcg gtgctttggt tgtgggggtt tacagcacaa cggcaactgg
    31801 tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt gtcctggacg atgggacatc
    31861 tttttccgca cgaccgaccc tttgtcgaaa atatcggcta taacttcctg catcatgcgg
    31921 cggatgactc attcccaagc gatcacggta cggtgatttt cacctttgca ctggcatttt
    31981 tatgctggca tcgcctgtgg tccggctcac ttttaatggt gctggccgtc gtcattgcct
    32041 ggtcgcgcgt ttatcttggc gtccactggc cgctggatat gctcggtgga ttgctggcag
    32101 gtatgattgg ctgccttagt gcccagatta tctggcaagc gatggggcat aaactctatc
    32161 aacgtctgca atcgtggtat cgcttctgtt ttgcattacc gatccgcaaa ggctgggtgc
    32221 gtgactgatc tctgctaaaa agtgtagtat tgagcggctc gcttcaataa ctattcagag
    32281 ggattatgga aacacgtcgc gaagagcgta tcgggcagct gctgcaagaa ttaaaacgca
    32341 gcgataagtt acatcttaaa gacgccgccg ccctgcttgg ggtttcggag atgacgattc
    32401 gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct cggcggctat attgttctgg
    32461 aaccgcgcag tgccagccat tacctgttaa gcgatcaaaa atcccgcctg gtggaagaaa
    32521 aacgccgggc ggcaaaactg gctgcgacac tggtagaacc cgatcagacc ctcttttttg
    32581 actgtggcac caccacgccg tggattattg aagcgattga taatgaaatc ccttttaccg
    32641 ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga gaaaccccat tgccgcgcat
    32701 ttctgtgcgg tggtgaattt cacgccagca acgccatttt caaacccatc gattttcagc
    32761 aaacgctgaa taatttttgc ccggatatcg ctttttattc tgcggcgggc gtgcatgtca
    32821 gtaaaggcgc tacctgtttt aatcttgaag agttgccggt aaaacactgg gccatgtcga
    32881 tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt tggcaaggtg cgtccggcgc
    32941 gcatgggtga cctgaaacgc tttgatattg tggtgagtga ttgttgcccg gaagatgagt
    33001 atgtgaagta cgcgcagacg cagcgcatta agttgatgta ttaatgacgt ataaccggat
    33061 gacgtttcgc gccatccggt tatcagaaga ttaagagaac cagctgccga accactgatg
    33121 gaatttcatc atcacgaaat cccacatccg accaaagaat ccgccctctt ccacattttc
    33181 catcacgatc agcggacgct gctcaatgga tttaccgtta agctggaaat caatggtccc
    33241 gacaacctga ccttttttca gcggtgcggt aagctgcggt tccgttaacg tgtagctcgc
    33301 cttcaggttt ttcagctgcc cacgcggtat ggtcactgag cccgcttcgc ctgccccaag
    33361 attcacttcg ctcttatcac caaaccagac gcgctgagtc acaaaggtgg catcaggttt
    33421 aattggcgtc acggtttcaa agaagcggaa accccaggtc aataatttct cagactcatt
    33481 aaaacggata cggtcagttt tcgcccccag cactacggag attaaacgca tatcgccctg
    33541 ggtagccgaa gcaaccagat tatatcccgc gcctgccgtg gttcctgtct tcatgccatc
    33601 aacattcaga ttgctgctcc acagcagacg gttacggtta ggctgacgaa ttttgttgaa
    33661 ggtgaactct ttctctttat gaatggcgta ctcttccggc acatcgtgga tcaatgcttt
    33721 acccagcaat gccatatcgc gcgcggtact gaactgcccc ggcgcatcca gaccgtgcac
    33781 cgtctggaag gtagtgttgg tcagacccag ttttttggca taaccattca tcaaaccaat
    33841 gaatgactcc tggctcccgg caacgtaatc ggccagcgca atacaggcgt cattaccgga
    33901 ctggataatc acacctttgt tcaagtctgc caccgaaacc tgatcgcccg gtttgaggaa
    33961 cattaccgat gaaccacgca gtgccggatt tcccgtcgcc caggcatctt taccgaccgt
    34021 caccatatcg gtgagtttaa tcttatcggc cttaagcgcc tgcccaacca catagctggt
    34081 catgatttta gtcaggctcg cgggatccag tttctcatcc gcgttgcctt ctgccagcac
    34141 tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca tccacgctcg gcgcttcaac
    34201 ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat aaaaatgcag aacccgctgc
    34261 aagaccacga aggagagagg agtattgcgt cataaatgcc acccgagtat ccattcaaaa
    34321 aaaatacgtt atatcgccgt atgataagtt cggtgattaa taacgcacac acaaacttaa
    34381 agaaaccgta agttggtaaa gtttttaaag tttatgcaat atcctgccgc ttcgctgctt
    34441 ctggtgtcat ttttttgcca gctgttgctt aaaagttaac tttacttcac catcggagtc
    34501 ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta tgattacgct gtggggtcgg
    34561 aataattcaa ccaacgtaaa aaaagtattg ctgacgctcg aagaactgga actaccttat
    34621 gagcaaattc tcgcgggccg tgagtttggg ataaatcacg atgctgattt tctggcgatg
    34681 aaccctaacg ggctggtgcc gttgttgcgt gacgacgaaa gtgatctcat tctttgggaa
    34741 tcaaacgcca ttgtccgcta tctggcggca cagtacgggc aaaaacgtct gtggatcgac
    34801 tcaccggcac gtcgtgcgga agcggaaaaa tggatggact gggcaaacca gacgctcagt
    34861 aatgctcatc gcgggatcct gatgggatta gtcagaacac caccggaaga gcgcgatcag
    34921 gccgccattg atgccagttg caaagagtgc gacgccctgt ttgccctgct cgatgcggaa
    34981 ctggcaaaag taaaatggtt ctctggcgac gagtttggtg tgggcgatat cgctatcgca
    35041 ccgttcatct acaatttgtt taacgttggc ctgacctgga caccgcgtcc aaatctgcaa
    35101 cgctggtatc agcaactcac tgaacgcccc gcagtgcgca aagtggtgat gattcccgtt
    35161 agctaattgc gtgggctaac tttaagtaat tccccactgg actcgtcggt gagaacgtat
    35221 aaataaccgt cgggtccagt gcgaacatca cgaattcgct gccctctgtc cgttaaaata
    35281 cggccatctt ctgtcacttt gtcgccgttg acgctcatca caatgacatc tttatctttc
    35341 agcgcgccaa taaataattt ttgctgccac tgggggaatt tatcgctgtt atagaaggcc
    35401 atgccgctca cagcgggcga atctttccag taaaaaacag gttgctcggt cccggcgacg
    35461 atctcccctt tcgcttccgg tatcttaaag cctgaatagt tgattcccca ggttgccagc
    35521 ggccagccgt agtttttgcc tttttgcggg atattaattt catcaccacc gcgcgggcca
    35581 tgttcattca gccacagtgc attactccac ggattcatcg ccattccttg cggattacga
    35641 atgccataag accagatctc ggcgcgcaca ccggattcct ttataaaagg attatcatcc
    35701 gggatttcgc cctggtcggt cagacgcacc agtttgccct gtaatttatc cagatcctgc
    35761 gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa gataaccttt accgtcgaat
    35821 accagccgcc cgccaaaatg gttgccggta gacagttttg gcatctggcg gaagacggtg
    35881 cggaagtcgg tcacttttga gagatcatca cttaagcggc cataacccac agcagttccg
    35941 gctttgccat catcgccaac ttcggaataa cttaaccaga tgcggcgaga ctgagcaaaa
    36001 tcaggcgcta aaaccacgtc cagcaggccg ccctgcccgt gcgcccaaac gtccggaact
    36061 ccggaaagcg gcgcagataa tccttttcct gcttgccagt gacgcaactc gccgccgcgc
    36121 agagtgatta acataccgtg attatcgggt aaaaaggcca gtgcccaggg atggtcgagt
    36181 ttgtcttgca gtacttcgac atttaccgtt gcaggagccg cccagagagc ggaagaaaga
    36241 caaataaggg gcacaaggaa aaaggattgt cgatgcataa cgcctccgga ttgactctgg
    36301 cttaaagcgt agtcagtgga ggagataaca aattcatttt tacaaaaact taaacatgaa
    36361 gggggagacg ctttctcccc cttagttttc aggccttctc aagcatggcg tgcttctgca
    36421 ggctctggat actcagcgtt aagctcatca gacaattttc aagcttatcg gcgttgacgg
    36481 taataacagt cgggcaatca tggtgcccac tcatcaaaca tactgcggct gtcgctaatg
    36541 cttcttcagc atgatgaaga gcactccact cttcctgatc cagatgaaga ttcaaccgca
    36601 gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt atctcgtaga tgattgcttt
    36661 cgctgacgga catgtatcct tttgccttcc tcagttgcac gtatgcagcg aggtcaacgc
    36721 gtgcgtcgat caaccggttc agcagatcga ttcgctgtct gtcaacgaac atacttcgat
    36781 cctcctcttt ccagccttgt tgcaattaaa tagtatggct attttttgag caaaatgctg
    36841 tccaggatca attcttcacg ccgaattaac aaaatttaca tttccttcgc ctccgccagc
    36901 gaatgagatt gcgatacaaa ttttatccag aactctgtag aatccctgcc ctgacctttc
    36961 ctgtaactga atatttttta agctatgagc aaagtaactc cccagccgaa aatcggcttt
    37021 gtttcccttg gctgtccgaa aaaccttgtc gattcagagc gtattctcac cgaactccgc
    37081 actgaaggtt atgacgtggt accgagctat gacgatgcgg acatggtgat cgtcaacacc
    37141 tgcggcttta ttgacagcgc ggtacaagaa tcactggaag ccattggtga agcgttgaat
    37201 gaaaacggca aggtaattgt gaccggttgt ctgggggcaa aagaagatca gatccgcgaa
    37261 gtccacccga aagtgctgga aattaccggg cctcatagct atgagcaggt tctggagcac
    37321 gttcatcact acgtgccaaa accgaaacac aacccattcc tgagcctggt gccagaacaa
    37381 ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa tttcggaagg ctgtaatcac
    37441 cgctgcacct tctgcattat tccgtctatg cgcggcgacc tggtgagccg tccgattggc
    37501 gaagtgttaa gtgaagcgaa acgtctggta gatgcgggcg ttaaagagat tctggtgatc
    37561 tcgcaggata cttccgccta tggcgttgat gttaaacatc gtactggctt ccacaacggc
    37621 gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt tatcgaaact ggggatctgg
    37681 acacgtctgc actacgttta cccttatccg catgtggacg acgtcatccc actgatggca
    37741 gaaggcaaaa tcctgccgta tctggacatt ccgttgcagc acgccagccc gcgcattctc
    37801 aaactgatga agcgtccggg ttctgtagat cgccaactgg cgcgcatcaa acagtggcgc
    37861 gaaatctgcc cggaactgac cctacgctca acctttattg tcggcttccc tggcgagaca
    37921 gaagaagatt tccagatgct actcgacttc ctgaaagaag cgcgtctgga tcgcgttggc
    37981 tgctttaaat acagcccggt tgaaggtgca gacgccaatg ccctgcctga ccaggttccg
    38041 gaagaagtta aagaagaacg ctggaaccgt ttcatgcagc tgcagcagca gatttccgcc
    38101 gagcgcctgc aagagaaagt gggccgtgaa attctggtga ttatcgacga agtggacgaa
    38161 gaaggcgcga ttggtcgcag catggcagat gcaccggaaa tcgacggcgc ggtttatctc
    38221 aatggtgaaa ccaacgttaa gccgggtgat atcctgcgtg tgaaagtcga gcacgccgat
    38281 gagtacgatt tgtggggtag ccgggtttaa ttaattcagg ccgggcaaaa tcatttgtcc
    38341 ggcctgcgaa taatgattac tgccagctaa tctgagccct gccgtttttc ttactcttat
    38401 acagccgctc atccgccagc attttcaagc gttcgacggt ggtacattca tttgaatgcg
    38461 ctacaccgat actggtactt aaattaatgc ttcccacctg gaaatgaacg atttctttta
    38521 gaatcgatag caactcttcc agcgttccac ccgtataaat caccgcaaat tcgtcaccgc
    38581 cgaagcggta gattttacct ttatctaccg ggatctgtga tttaaggatt ttcaccactt
    38641 tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc gttaatgttt ttgaatttat
    38701 cgacgtcaaa caggcacaaa taaccgcctt tttccggcag cgcttttaaa tcttcttcaa
    38761 aggattttct gcccattgct ttggtcaggt cgtccaggaa cgccgagtta tacatactgt
    38821 agagtttttt gacatcggca gccgaaaaga aaagcccgcc atccagtacg cgttcaaaat
    38881 ggttattaat gatggcttct ttctccacaa taccacgatt aatcactctg cgtaccagat
    38941 agaggcaact ggtacccgat atcactgcca ccaatgcggc aaagaaaata atcaatgtac
    39001 agacccgcaa ataaacatct tttttatacg caaatacgta caatccggtc ggcaaatagg
    39061 cgaagtgctc catcgcttcc gaaacggtga agatggcgtt tttagcatta tattcatcac
    39121 cggtcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg tccatcgcca
    39181 gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga ctacgattgt
    39241 gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata aaccctgtat
    39301 ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt ttctcgaact
    39361 cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg tacaaataag
    39421 agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt tcctccgtgg
    39481 gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca atatttgcca
    39541 ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc tttaccattt
    39601 gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga ctgactgtaa
    39661 gtacgaactt attgattctg gacatacgta aactactctt ttactaattt tccactttta
    39721 tcccaggcgg agaatggcat gggtttctgg tacagatacc cctgagcctg aacacatcct
    39781 aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat caccgggatc
    39841 cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt accgaaaata
    39901 tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata aaaacgtttc
    39961 aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg tttacgcagt
    40021 tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc agtgatctca
    40081 acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa acgctcatga
    40141 aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa atcatccttc
    40201 agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat aatctgataa
    40261 cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag cggtgaaatg
    40321 atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgcccagaat ggcgcgggtg
    40381 ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat tgcatggcga
    40441 atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc atcacaaaaa
    40501 ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag tttttctgcg
    40561 taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg gatgcgcaga
    40621 ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg ggtaaatgtc
    40681 tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac gatatcgcag
    40741 cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt attgcgttca
    40801 tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat ccccacagcc
    40861 agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc gctttcctga
    40921 tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc tttcatttcc
    40981 gcgatcgaat ttttgatgct gataatctct ttcgcatcgc cttcgcgaat tttcagagcc
    41041 tcgatttgat ccgttgagtc gggcaaaaac gccagcgtat taaccagctc ggtcatttca
    41101 gaaaattgcc gacggaacac acgagtggtc atgaaatata ccaccagcaa cacgataata
    41161 atgaaagcaa acggtagata aaaaagttta tctgatgcgt tcaataaatt accgccagga
    41221 taataaaaat agatttcgaa caatgcatta tttggcactg ctgatgtctt tgcaatatac
    41281 tccgtatcga cagaatcaat aatattataa ttaccttcgc tattataaat aatcagtttt
    41341 tcacgagcga gttgattctt ggcaatgatc tcatgacctt gtttcaaaaa tagaacttca
    41401 tcattagcgc tgatttgatc aagatactcg ttcagatacc gccagtttaa atcgacatga
    41461 attttatcta ccgctaacaa atcgtgatct ttgccgttga tacgtagcgt aaaagagata
    41521 cattttttac ccggacgatc aaagcaattg tatgccggat tccagctgtt gttagagtca
    41581 tctgaagcca ccgaccacgg tcgtttcgat gggttaaaat cggcaaaaat aacccgccct
    41641 gactcgtcga caagcggtcc atttacttgt tcctgatgat aaacaacgat tttatcattc
    41701 gccctccgcg aaaagagata atatttatta tcatcaagta ccagtcccac cgagtctaaa
    41761 aatggcatca tttccatttt cagaatcacg gcgcgttgaa tctcctctgt attttttaat
    41821 aaatacagtg agtcctttaa tgtcagggcc gaacgttcaa tatctttaaa gaaatagcca
    41881 acgtttttct gaataagttc aaagcggcta tcggcacgtt ttaccgcgcg ttccgatacc
    41941 agttgatagt taatgatgaa aaaaagacca ataaatgaca gtgctgccaa taaaaataaa
    42001 attatcagcc ttatctttat cttttcgtat aaactcagca tgttaataca tttaacaaaa
    42061 taactatctg aacgctgtgc cgctgcaaaa atagtccctt gctgcggctt tttaaccaaa
    42121 ttacccgatt ctacaaaatc agcagaaagt tacaacactt gcttagggca ataattgcct
    42181 gttaggtgat taccctttca agcaatattc aaacgtaact atcctttaat tttcggatcc
    42241 agcgcatcgc gtaaaccatc gcccaacaaa ttgaacgcca gtacggtcag aaaaatagcc
    42301 agggccggaa aaacagcgac atgcggcgcg ataaccatat ccgctcgtgc ctcattgagc
    42361 attgctcccc actctggtgt cggcggctgc gcaccgaggc cgagaaatga caggctggcg
    42421 gcagagataa tcgaggtgcc aatgcgcatg gtgaaaaaca ccacgataga cgagacggtc
    42481 ccaggcaaga tatgacgcaa taaaatggtc atatcgctgg caccaatact gcgtgctgac
    42541 tcaataaagg tttgctgttt caacaccagc gtgttgccgc gcaccaggcg ggcaaacgcg
    42601 gggatggaaa aaatggcgac tgcgataatc acgttagcaa tgccgcttcc caacaccgca
    42661 acaacagcga tcgccagtaa aatacccggg aaggcaaaca gcacatcgca aatgcgcatg
    42721 atcagccgat cccaccagcc ttcataatat ccagccagca agcccagcaa cgtccctatc
    42781 gccgcaccga taaacacggc aaacacgccc gccgccagcg agatttgcgc accaaccagg
    42841 acacggctga aaatgtcgcg ccccagtgaa tcgacgccaa accagtgctg caaagaaggt
    42901 ccgttattca gattgtcata atcaaaataa ttttcggcgt catagggagc gatccagcgt
    42961 gcaaaaatgg ccaccacaat caataaaata acgaataatg cggcggtcat cgccatatgc
    43021 tgacggcgaa atcgtcgcca gaattcatgc cacggtgtac gcacctggtc aggtttgacc
    43081 agtggcatgg cgtttaacac cgcctgacgt cgccagttaa atagtcgcat ccttacttgt
    43141 acctgatagc cgggttaatg gcggcgtaaa gcacatccac cactaagttg ataagaataa
    43201 attccagcga gaaaagcaga atttccgcct gaatcaccgg gtaatcgcgc atttctacgg
    43261 agtcaacgag taagcgtcca agtccgggcc agttgaaaac tttctccacc acgatagaac
    43321 caccgagcaa aaagccaaac tgtaagccca tcatggtcac taccgggatc atcgcgttac
    43381 gtagcccgtg tttgaggaca acccaggttt cgctcacccc tttcgccctc gcggtacgca
    43441 tataatcttc gcttaaaaca tcgacaaacg acgcgcgggt aaagcgcgcc atcacggctg
    43501 ccactgccgc gccgagcgtc agggagggta aaatgtagtg ctgccaactg tctgctccca
    43561 cggtaggcag ccagcccagt tcaacggaga atacctgaat taaaagcatc cccagcgcaa
    43621 atgccggaaa cgagatcccc gacaccgcaa tggtcatact caatcgatcc ggccaacgat
    43681 tacgccagac ggcggcgata attcccgccg ccataccaaa tatcaccgcc cagaccatac
    43741 tggttatggt cagccacagc gttggcataa agcggctggc aatctcatcg gcaaccggac
    43801 gacgcgacac catcgacagg ccaaaatccc cctgcacagc attgctgata tagtgccaga
    43861 actggtgata cagcggctga tccaacccca gctgctgacg caccagttct ataacctgcg
    43921 catcagcttc gggcccggca atcaatcgcg ccggatcgcc gggcagcata tgcacaaata
    43981 aaaacaccag caccgagacg ataaacagcg tcggaatcaa ccccagtaag cgtttgataa
    44041 cgtaattaag cattccactc cctgcgttgc ttattgcaaa tccgcgtctt caaagctgaa
    44101 gccggtgtct ggcatgatcc aaaaaccggt caggttttta ctgtgtgccg acaccagttt
    44161 ttctaccacc agcgggatcc acggcgactc ttgccagatg atatcctgcg ccgccttata
    44221 caagcgggtc ttttccgccg gatcattagt tttcagtgcc tgagccagga agtcgtccac
    44281 ctgtttattg ctgtaaaatg cggtattaaa cagcgtcggt ggccagtttt gcgaggcgaa
    44341 cagtggcgat agcgcccagt ccgcttcgcc ggttgaagcc gaccagccag tgtagaacat
    44401 ccgcacgccg ctctcttttt gccctttacc ttcaacttct gccgcccgct gtccggcatc
    44461 catcgcagtc acctggactt taatcccgac ctgcgctaac tgctgctggg taaattgcag
    44521 cactttctgc gcggtgctgt ggttatgtga cgaccacagc gtggtactga aaccgttggg
    44581 atatcccgcc tctttcagta attcgcgcgc tttcactgga tcgtaaggcc acggtttata
    44641 actttgcgcg taggcgatac ttggcggtac cacaccagta gctggcgttg catagcccgc
    44701 aaaggcaact ttcaccagcg ccggacggtt aatggcgtaa ttcagcgcct cacggacctt
    44761 cgggttatcg aacggctttt gcgtcacgtt catactgata taacgctgca taattgacgg
    44821 actggccatc aactcgatat ttttgttttt ctccagcagt gtggcctgct cgtagggaat
    44881 ggggaaagca aactgcgctt caccggtttg cagcattgcc gcgcgggtgt tgttatccgc
    44941 caccggacgc caggttatgc tgtccagttt gggcaatcct ggctgccagt aacccgcgaa
    45001 ttttttcacc ttcacaaaat cggtctgatt ccaggtatcc agttcatacg gtccggttcc
    45061 caccggatga aaaccaatct ccttgccata tttttccagc gctgccggtg aaatcatcgc
    45121 ggtcgccgga tgggcaagaa tattaataaa cgctgagaac ggctgtttga gggtaatctt
    45181 tactgttgtt ggatcgatcg cctccgtttt ggcaatattc ttatacaggt tatagcgttt
    45241 aagatgattc gccggatcgc tggcccggtc cagattcgct ttcaccgccg cggcgttgaa
    45301 atcggtgcca tcctggaatt taatgccttc ccgcaatttc acggtgtaag taatgccgtc
    45361 atcggaaacg gtataactct ccgccagcac gtttttcagt ttcatctctt tatccagacc
    45421 gaacagcccc tggtaaaacg atttcgctac ggcctgagat aacgtgtcat tcgcgtcata
    45481 cggatcgagc gtggtgaaat tcgatcctac cgccaccacc acatctttgg cagcgaatgc
    45541 acaagatgcc atcaacgctg tcgcaatgcc cagcgccact aacccactac ggtgtacagc
    45601 tcttgccatt ttattctccg cctgaatgtt atctacgcat gaatgcgtat tctgattgtg
    45661 gttgtgcgac gtaatgcccc ggcccgacgc attgcaacga gacggctgcc acctcttcgc
    45721 cacgcagatg aatattgctg ggaagatcgt ccgacagcag tacacgctgc ggtcgttgtc
    45781 gggacggttc agcgaccgga actgccgcca gtaatttacg cgtataagga tgctgcgggt
    45841 tttcgaagac cgcgcgccgt ggaccaattt caacaatttg cccgagatac atcaccgcca
    45901 cacgatgact aatccgctct accacagcca tatcgtggga gataaacaga tacgcaatgc
    45961 cgaaatcacg ctggagatcg agcaacaagt tgataatctg cccacgaata gaaacatcca
    46021 gcgccgaaac ggcttcgtcg gcaatgatca cttttggatt caatgccaac gcgcgagcaa
    46081 tgcagatgcg ctggcgctga ccgccggaaa actcatgcgg gtaacgccag gcatgttcag
    46141 gtaacaggcc cacgcgctcc agcaaccacg caacgcgtgc agccgcttct ttacctggca
    46201 ataaaccgtg tacacgcagc ggttcgataa tcgaatcacc gatggtctga cgtgggtcca
    46261 gcgaagcgta agggtcctga aaaataaact gaatatcccg gcgcaatgcc tgaagtttgc
    46321 cgggtgacaa ggtatcgatt cgctgaccgt taaagataat ttcgccgccc tgcgattcga
    46381 ccaggcgcag caacgcccgc ccggtagtgg atttaccgct gccagactcg cccaccagcg
    46441 atagcgtttc gccaggccag agatcaaaac tgactttctc aacggcatgc acttcccgcg
    46501 ttacgcgatt caacaaaccg ctgcgcaaag ggaaacgggt gaccagatta cgcacccgta
    46561 aaacaggttc accatccacc accgttttct gctcgatggg gggggcctgt ttcgctggat
    46621 gttcaagcga tatcaatggg aaacgtcggg gataatctaa ccctttcatc gcaccaagtt
    46681 gcggaacagc agctaacagc gcacgggtgt aaggatgttg cggtgcatga aaaatctgtt
    46741 cgacggtacc cgtttccacc gcctcgccct gatacatcac cagtacccga tcggcaatct
    46801 ctgccaccac gcccatatcg tgagtgataa agataacgcc catcgacatc tctttttgca
    46861 atactttgat taattgcagg atctgcgcct gaatagtgac atccagcgcg gtggttggct
    46921 catcggcaat cagcaccgcc gggcggcatg acagcgccat cgcaatcatc actcgctggc
    46981 gcatcccgcc agagagttga tgcggataac gtgaaagaat ggtttgtgcc tcaggaatgc
    47041 gtacctgatc cagcatccgc ttcgcctcga ccatcgcttc ttcacgactg gcgttctgat
    47101 gcagacgaat tgattcggca atctgttcac ccacagtaaa taccgggttc agcgatgtca
    47161 tcggctcctg aaatatcatc gccatatccg caccgcgaac atggcgcatt tgtgcagcgc
    47221 tctgctcgct aagttcaatc acttcgcgac tgcgccgctg caacagcatt ttatcgcact
    47281 gtactaaacc gcccgcctgt tccaacaggc gcatcaatgc caacgcagtc actgacttac
    47341 aggagccgga ttcgccaaca attgccagcg tctcaccgcg ttgcagacta aaagagagat
    47401 tgcggaccgc agctattttc tgctggtcct gcataaaggc aatattcaga ttttcaaccg
    47461 ccagcacatt accggcatca agttcatcac tgtgtggcaa cggtgtcccc tttttcacgg
    47521 tagataccgg tggttggcgt atcgcctgcg tagccccagg cgcgatacat tccttcggtg
    47581 ttaaacggta gcgcgacatt cccttcatgg tcgatagcga ttaagccacc gctaccgcca
    47641 agcgcaggga gtttttccat tactacccgc tcgcaggctt ccgcgagact taatccgccg
    47701 taatccatta acgcggcgat gtcatatgcc gccagcgcgc ggatgaagac ttcgcccgtg
    47761 ccggtacaag aaaccgccac actggcgtta ttggcgtagc atccggcacc cactaaggga
    47821 ctatcgccaa ctcgtccggg taatttattg gtcattccgc ctgtggacgt ggctgccgcc
    47881 aaattgccgt ctaaatccaa cgccacggcc cccacggtgc ccattttctg tttttcatcc
    47941 agtggcgcac cgctatggtc gaggacggtt gccccttcct tgcgcgctgc cagtagttgt
    48001 tcataacgca aaggcgtgga gaaaatctcc ggcgagacgc gctccatgcc acgagcaaac
    48061 gcaaaatttt ctgccccttc gccaatcatc atcacatgcg ggctttgctc catcaccagc
    48121 cgggcggcaa gaaccggatt acgcagatga ctaacgcccg ccaccgcacc ggctttcagg
    48181 gtgttaccat ccatcacaca ggcgtccagt tcatgggttt catcacgcgt aaagacagcg
    48241 ccaattccgg cgttaaacag tggacactct tccagcagac gcaccgcttc cgtcaccaca
    48301 tccagcgcac tttcgcccgc ttccagcatt ttctgcccgg tttcaacaat ggcagacaac
    48361 gcctcaatgt agcgtaattc ctgttgcaga ctcatctgcg cgcggctaat tgcacctgcg
    48421 ccaccatgaa ttgcaatgac tgctttgccc atagtattcg tccattaagg gtggctcgcc
    48481 acttttgcta taaatatcag aatttttatg aattactcat tcaatttttg aatatagaaa
    48541 gatggatatg gcatgtaaag gcaggagcca acgctacgcc cgaggtggca tacagtctgt
    48601 ggcgtttttc tgacataata ggcaaattcg attttgcctc cgcaggagtg ttttcatgga
    48661 atttaccacc ggattgatgt cgctcgacac cgcgcttaat gagatgcttt ctcgcgtcac
    48721 cccactgacc gcccaggaaa cgctgccact ggtacagtgt tttggtcgta ttctggcgag
    48781 cgatgtcgtt tcgccgcttg atgttccggg gtttgataac tccgcaatgg acggctacgc
    48841 ggtgcgttta gccgatattg cctccaggca accgctgccc gttgccggta aatcctttgc
    48901 cggtcagccg taccatggtg aatggcctgc gggtacctgc attcgtatta tgaccggtgc
    48961 gccggtgccg gaaggctgcg aagcggtggt gatgcaggag cagactgaac aaacggacaa
    49021 tggcgtgcgt tttactgctg aagtgcgtag cgggcaaaat attcgccgtc gcggtgaaga
    49081 tatctctgca ggtgcggttg ttttcccggc gggaactcgc ctgactaccg ctgaactgcc
    49141 agtgattgct tcactgggga ttgccgaagt tccggtgatt cgtaaagtgc gtgtagcgct
    49201 tttttctacc ggtgatgaac tccagttgcc cggtcagcca ctgggcgatg gccaaatcta
    49261 cgataccaac cgtctcgccg tacacctgat gttagaacag ttgggatgcg aggtaatcaa
    49321 cttagggatt atccgcgacg atccacatgc cctgcgcgcc gcatttattg aagccgacag
    49381 ccaggcggat gtggtgatca gttccggcgg tgtttcagtg ggtgaagcgg attacaccaa
    49441 aacgattctt gaagagctgg gggagatcgc cttctggaag ctggcgatta aaccaggtaa
    49501 accgttcgcg ttcggtaaac tcagcaatag ctggttctgc ggcctgccgg gcaacccggt
    49561 ttcagcgacg ctgaccttct atcaactggt acagcctttg ctggcaaaac taagcggcaa
    49621 caccgccagc ggcctgcccg cgcgccagcg cgtacgcaca gcgtcccgcc tgaaaaaaac
    49681 gccaggacgt cttgatttcc agcgcggcgt gctgcaacgc aacgccgacg gtgaacttga
    49741 agtgacgacc accggacatc agggttcaca tatatttagc tcctttagtc tcggcaactg
    49801 ctttatcgtg ctggaacgcg atcgcggcaa tgtggacgtg ggcgaatggg tggaagtaga
    49861 accgtttaac gcgttgttcg gaggcctgta atggcggaac tcagcgatca ggagatgctg
    49921 cgctacaacc ggcaaatcat tctgcgcggt tttgattttg acggtcagga ggcgctaaaa
    49981 gattctcgtg tgctggtagt cggcctcggt ggactcggct gcgcagcttc gcagtatctg
    50041 gcaagcgccg gtgtcggcaa cctgacgctg ctcgacttcg acacggtttc gctctcgaat
    50101 ctgcaacgcc agacgctgca cagtgatgct acggtcgggc aaccgaaggt agaatccgcc
    50161 cgtgacgcca tgacgcggat caacccacat atcgcgatta cgccagtcaa tgcactgctt
    50221 gatgacgctg aacttgctgc gatgattgca aaacacgatc tggtactcga ctgtacggat
    50281 aacgttgcgg tgcgtaatca actgaacgct ggctgttttg ccgcgaaggt gccgctggtt
    50341 tccggcgcgg caattcgtat ggaagggcaa atcacggtct tcacttatca ggacggcgaa
    50401 ccgtgctatc gctgccttag ccgtttgttt ggcgagaacg cattaacctg cgtagaagca
    50461 ggcgtgatgg caccgttgat cggcgtaatt ggttcgttgc aggcaatgga agcgatcaaa
    50521 ctgctggcag gttatggcaa accggccagc gggaaaatcg tcatgtacga tgcgatgacc
    50581 tgtcagtttc gcgaaatgaa actgatgcgc aatccggggt gtgaggtgtg cgggcagtaa
    50641 ttgccacctc caggccagat aagacgcggc actccgccac gtcatcagca atttatgagg
    50701 atgcagaacg tccggttaaa tcgacgttct gccaaacgct cccagccagt cctgttcaaa
    50761 cttcgccaca gcggcttcca ccgccggata gctaatcatc tgttgtgcca catccagtgg
    50821 cagcgtaatt gattcacatc ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa
    50881 actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt gtaagtcggt
    50941 cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag gcgcaacata
    51001 ttccgcacct gccagcgccg acagcagccc ttgtgctgcg ccatataccg cggttcccag
    51061 cgttggaatc ccttccgctt ttaacatctt aatagctgcc agcccctcgg cggtcaccgg
    51121 aactttcacc acgatatccg caataataga acgcaattta cgtgcgtcat tcaccatccc
    51181 ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg
    51241 aagttgcgga agcacaacat ccagcggttt tttacccgcg gcgataatgc ttgggttagt
    51301 ggtcacaccc gccagcggaa aaatacgtga cagcgccttc accgcaacaa cgtctgaagt
    51361 atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat gtagactatc
    51421 acgggaaaac tctctgaaca gttgacgcac atcaatctaa ctttcattcg aaagtatttt
    51481 aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct actcgaccca
    51541 tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg
    51601 gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg ctgtatgacg cacgactgtg
    51661 tctggaaggc tgcgagctgt gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa
    51721 tggtttgctt attcatcggg aaaagttaac cccggagcat ctgacggcgt taaccgactg
    51781 ctgtccgaca caggcattaa ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatgac
    51841 gaccgttctg cgcgataaac cgttttacga tcgcagcggc ggcggtttaa cgctttcggg
    51901 tggtgagccc tttatgcagc cggaaatggc gatggcgcta ctgcaagcca gccacgaggc
    51961 aggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata tcgccccttc
    52021 tctgccctat atcgatctgt ttcttgccga tttaaaacac gttgccgacg cgccgtttaa
    52081 acagtggacc gacggtaacg ccgccagagt gctggataac ctgaaaaaac tcgccgcagc
    52141 gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg acgaaacctc
    52201 tgtaaaagcc attaccgatt ttgccgccga cgagctgcac gttggcgaaa ttcattttct
    52261 gccctaccac acgctgggca tcaacaaata tcacttactt aatctgccct atgacgcccc
    52321 ggaaaaaccg cttgatgcgc cagaactgct cgactttgcc cagcagtatg cctgccagaa
    52381 agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact ggacacgctc
    52441 agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt
    52501 accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa gccgatcccg
    52561 gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac
    52621 gatgagttga tcattggcaa ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa
    52681 tatactgtct cgtggatcga aaaagagatt gatgatctgg cagatcgtcc cggtgctggc
    52741 tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaag tgtgcccgtg gtggcgcgat
    52801 cagaccgtac aggatcgctg ctacggcatg tttaccgatg agcaaaaagg tctgctggcg
    52861 accggaatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct ggcggtgaat
    52921 ttcccgctgc tgctggaaaa aggtcttgat ggtctgcgtg agaaagtagc ggaacgtcgc
    52981 tcgcgcatca atctgacggt gctggaagat ttacatggcg aacaattcct gaaagcgatt
    53041 gatatcgtgc tggtggcagt cagtgaacac attgaacgtt tcgctgccct ggcgcgtgaa
    53101 atggctgcta ccgaaactcg cgaaagccgt cgcgatgaac tgctgacgat ggcagaaaac
    53161 tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca actgtgttac
    53221 ttcatccaat tgattttgca gatcgaatct aacggtcact cggtatcgtt tggtcgtatg
    53281 gaccagtatc tctacccgta ctatcgccgc gacgttgaac tcaaccagac gctggatcgc
    53341 gaacacgcca tcgagatgct gcatagctgc tggctgaaac tgctggaagt gaacaagatc
    53401 cgctccggct cacactcaaa agcctctgcg ggaagtccgc tgtatcagaa cgttaccatt
    53461 ggcggacaaa atctggttga tggtcaacca atggatgcag tgaatcccct ctcttacgcg
    53521 atcctcgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt gcgttaccat
    53581 gcaggaatga gtaatgattt cctcgacgcc tgcgtacagg tgatccgttg cggcttcggg
    53641 atgccggcgt tcaacaacga cgaaatcgtg atcccggaat ttattaaact cggtattgaa
    53701 ccgcaggacg cttacgacta cgcagcgatt ggttgtatcg aaaccgccgt cggtggcaaa
    53761 tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat gctggcggcg
    53821 ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc tgccacaaga aaaagcgttg
    53881 tcggcaggca actttaacaa tttcgatgaa gtaatggacg cgtgggatac gcaaatccgt
    53941 tattacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac
    54001 gtacacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc
    54061 aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggttggcat tgccaacctc
    54121 ggcaacagcc tggcggcagt gaagaaactg gtatttgaac agggcgcgat tggtcagcaa
    54181 cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca actgcgtcag
    54241 cggctgatta acggtgcgcc gaagtacggc aacgacgatg ataccgtcga tacgctgctg
    54301 gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataaccc gcgctacggt
    54361 cgtggtccgg ttggcggcaa ctattacgcg ggtacctcgt ctatctccgc taacgtaccg
    54421 tttggcgcgc aaactatggc aacaccggac gggcgtaaag cccacacccc gctggcagaa
    54481 ggcgctagtc cggcctctgg tactgaccat ctcggcccta ctgcggtcat tggctcggtg
    54541 ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc tcaaccagaa actgaatccg
    54601 gcaacgctgg agaacgaatc tgacaagcag aaactgatga tcctgctgcg caccttcttt
    54661 gaagtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac gctgctggag
    54721 gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg ctattccgcc
    54781 ttcttcaccg cgctctctcc agacgcacag gacgatatca tcgcccgtac tgaacatatg
    54841 ctgtaatctc ctcggcccgg cggcgtcgtc gccgggctaa atcattcaca tcatcaattt
    54901 catccttact ttcattcgaa atataatttg tgctctgcgt cacattgtta ttacactgtt
    54961 acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg tcacagacat
    55021 ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacatttta tggcgcaata
    55081 tcaggaactg aaaaagcgcg gcattaagtt cgttgtcgcc agcggtaatc agtattacca
    55141 gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg aaaacggcgc
    55201 actggtttac gaacatggca agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg
    55261 gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct gcggtctgca
    55321 aagtgcatat gtcagcgaaa atgcccccga agcatttgtc gcactgatgg caaaacacta
    55381 ccatcgcctg aaagctgtaa aagattatca ggagattgac gacgtactgt tcaagttttc
    55441 gctcaacctg ccagacgaac aaatcccgtt agtgatcgac aaactgcaca tagcgctcga
    55501 tggcattatg aaacccgtca ccagtggttt tggctttatc gacctgatta ttcccggtct
    55561 acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt
    55621 ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc gttattcctt
    55681 tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc cgttacgcta ccgatgataa
    55741 taatcatgaa ggcgcgctga atgtgattca ggcggtgctg gataacacat ccccttttaa
    55801 cagctgacct ctctaccgga gccgtgcgct ccggttttcc cttcttttta catcctgtac
    55861 atcacatcaa atcattaaac ttgcattaac ttatttataa cttatattgt cacatgcaaa
    55921 tcagaatggt ttcgaatgaa agttaatgag aggtggttat ggcttctact tttaccagcg
    55981 acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc
    56041 agcttggcga cgtccagcag atctttaatc agctaagcga tgacattgcc acgcgagtgg
    56101 ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg tcttatgccg
    56161 atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt cgcggttgtg
    56221 cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg
    56281 actatctgga ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat aatttcttcg
    56341 ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag gcgcaaatgc
    56401 aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat
    56461 ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac cctgaccgta
    56521 tccgccgccg tccgcccgga acgacctcca aaggtcttgg agcgcatacc gactccgggg
    56581 cgctggaacg ctggctgctt ccagcgtatc agcgcgtttt cgccaacgtc tttaatggca
    56641 atctggcgca atatgatccc tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg
    56701 tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggaca gcgctctctg
    56761 atatgctgcc tggtcagggg ctgctgcacg ttgtgcccat tcctgaagcc atggcgtacg
    56821 tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc gtagcgcccg
    56881 gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg ttaaccagca
    56941 ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc cattccgttg
    57001 cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg gcaccgatgt
    57061 gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc
    57121 cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt acgcttgccg
    57181 acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta atggaagtga
    57241 attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca agtgcgcttt
    57301 cttaatgcct gatgcggcgt taacgcctta tctggcctac aaagtcctgc agattcaata
    57361 tattacaggt actgcgtagg cctgataagc gtagcgcatc aggctatttt gcatttgtca
    57421 tcagccgccg tcaccccgac agtatcgggg tgacggataa tcacttactc gatcccttta
    57481 ctacgcaggt aatcttcgta attaccgcta aagtcgatca cgcgttccgg ggtgatttcc
    57541 agaatgcggg tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg
    57601 ccctgataca gttccagcgc catgttcagt gactcgatgg attccatatc caggtggttg
    57661 gtcggttcgt ccatgatcag aatgttcggc ttctgcatca ttaacttacc aaacagcatc
    57721 cgcccttttt ccccaccgga aagcacttta gctggctttt tgatgtcgtc ctggctgaac
    57781 agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg cttccactga
    57841 ctcatccatt cgaacacggt cagatcattt tcaaactcat attcgtggtc ctgggcgtag
    57901 taaccaatgc gcgcgttttc agaccatttt acggtgccgc tgtccggttg cagatcgccc
    57961 accagcgttt tcagcagcgt tgatttaccg acgccgttgg tacccagtac cgcaagtttt
    58021 tcaccgacct ccagcagcag gttgagattt ttaaacagtg gaccgttatc aaaccctttg
    58081 gtcagacctt ccacttccag cgcgttacgg aacagtttct tatcctgttc aaaacggatg
    58141 aacgggttct gacggctgga ggctttcacc tcttccagtt tgattttatc aatctggcgc
    58201 gcgcgcgaag ttgcctggcg agatttcgag gcgttggcgc taaagcggct aacgaaagat
    58261 tgcaactcag caatctgcgc tttcttcttg gcgttatcgg ccagcagacg ttcacgcgcc
    58321 tgggtcgccg ccgtcatgta ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa
    58381 tccagatccg ccatgtgcgt acagaccatg ttaaggaagt gacggtcgtg cgagatgatg
    58441 atcatggtgc tgtcacgctc gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg
    58501 aggttgttgg tcggttcgtc gagcaggaga atatccggat cagcaaacag cgcctgcgcc
    58561 agaagcacac gcagcttcca gccaggagca acttcactca tcgggccgta gtgctgttcc
    58621 actggaattc ccacgccaag caacaattca ccggcgcgag cttccgcaga gtaaccgtcc
    58681 atttcgccgt atttaacttc cagatcggcc actttatagc cgtcttcctc actcatttcc
    58741 ggcaaagcat agatgcggtc acgctcctgc ttcacttccc acaactcttt atgccccatg
    58801 atcaccgtat ccagcacagt gaactcttca aaggcaaact gatcctgacg cagcttacca
    58861 atgcgctcgt tgggatcgag ggaaacgtta cccaaagtcg gctctaagtc gccgccgagg
    58921 atcttcataa aggtggattt accactaccg ttcgcgccaa tcaggccgta acggttgccg
    58981 ccgccaaatt tgacggaaat gttttcaaac aacggcttac tgccgaactg catggtgacg
    59041 ttactggaaa ctaacacagg cgtatcctga aaagagatat gacaaaccgc gcattatgcc
    59101 ataagtctga aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa
    59161 aatgtgattt cgtacacatc tgatttcact gtgagctgga atgaacttat aatgcgcttc
    59221 caatactcta atattctcaa tccaatggcc tgccaggcac aaaatctcgc ttaacatgaa
    59281 tatgaaattg aaaacattat tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc
    59341 ctctgcggta acttatcctc tgccaaccga cgggagtcgc ctggttggtc agaatcaggt
    59401 gatcaccatt cctgaaggta acactcagcc gctggagtat tttgccgcgg agtaccagat
    59461 ggggctttcc aatatgatgg aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg
    59521 tactgtactg aacattccgc agcagctgat cctgccggat accgttcatg aaggcatcgt
    59581 cattaacagt gcagagatgc gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt
    59641 gctgccgatc ggcattggtc agttaggcaa agatacgcct atcaactgga ccaccaaagt
    59701 tgagcgtaag aaagcaggcc cgacctggac gccgaccgcc aaaatgcacg cagagtaccg
    59761 cgctgcgggc gaaccgcttc cggctgtcgt tccggcaggt ccggataacc cgatggggct
    59821 gtatgcactc tacatcggtc gcctgtacgc tatccatggc accaacgcca acttcggtat
    59881 cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt
    59941 cgagaaagta ccggtcggta cccgcgtaca gtttattgat gagccggtaa aagcgaccac
    60001 cgagccagac ggcagccgtt atattgaagt ccataacccg ctgtctacca ccgaagccca
    60061 gtttgaaggt caggaaattg tgccaattac cctgacgaag agcgtgcaaa cagtgaccgg
    60121 tcagccagat gttgaccagg ttgttcttga tgaagcgatt aaaaaccgct ccgggatgcc
    60181 ggttcgtctg aattaatctt caaaacttaa agcaaaaggc ggactcataa tccgcctttt
    60241 ttatttgcca gaccctagtt ggccgggagt ataactaaca aaacatatcc caccaacgcc
    60301 gcccacaaca gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc
    60361 gccatacgta gcgcaatcaa atttgccagc gatccgggta ataacccaaa gccacctacg
    60421 tttaccgccc ataccagtaa taaagacggc ggcacatagt tcagcaacaa tatggtactc
    60481 ggcacgttac tgatcacctg cgataaaccg attgccgtta accataaccc gggttcagat
    60541 agatgactca cgttacccaa cacgccttgc aacgctggaa gctgggtcag taaatggacg
    60601 tcgataaaca tcgccataaa caccagcagc agcgtccagt ccacactgag caccacgcga
    60661 cgtgccagca gcgcaaagcc tgccgccaca atcaccagtc cccacagctc ttgtttgaac
    60721 tccagcgccg tcagaaagac gatatacagc cccagacaac tccacaccag ccgcggtttc
    60781 cactccggtg tttgcacccc cgtatggtat tgcagcgcct ttccagggaa acaacaccag
    60841 cacaggagca ggagcgtcag catcattgcg ccagccagcg gtgccatttg ggcaataaat
    60901 ccggcaaacg aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc
    60961 gtcagtagcg aaccagcgtt gactgccagc gcctcaaaaa taatcagccg attaaccggg
    61021 atctcacaca gtctttttag cgtgatagtc agcggaacaa caataaacag cgcgacatcg
    61081 ttggtcagaa aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc
    61141 tccgtagcaa agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc
    61201 acacctttgg tcagcagcat caggccgctt aaggtgatga tggtgtgcca gtcgatagca
    61261 gcaggccagg atttcggtgc aaagggcacg aaaaagctta atccgatacc aacaagaatt
    61321 aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct catttggcac
    61381 cgtgtttctg ggtaaacaaa cgaaaagcgt cgagcgtctc ttcactaaca tggtgctcca
    61441 tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc caacaccaac aagaaatttt
    61501 cgactatctg atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa
    61561 acacgccacg ccaggggatc atttcaatca gccccatggt tgccagccgc ttaagcattt
    61621 tagccaccgt cggttgcgaa actcccagac gagcagccat gtccacctga cgagcttccc
    61681 ccacttccct gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat
    61741 gcgcctctct gacctggcgg aacccttcaa cgtgctcttc cacgttcact aactgcgtca
    61801 ctttttttgc tgttggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg
    61861 catacatagc gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa
    61921 attaacgttt tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat
    61981 catccctcaa ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg
    62041 tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt taatgctgct ctctatgttg
    62101 tgcgatatgg tcaacaacaa accgcagcaa gataaacctt ccgataaata gcggcgtcgc
    62161 ggtacgccgc ttcactcctg ctttcatgca ggcataacgc gttttggtct gaaaaacccc
    62221 actttttgtc ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga
    62281 acggcggctc gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc
    62341 cctttccact cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg
    62401 gcgtttgtta cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag
    62461 ccgggtattt agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg
    62521 cttaggttac cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct
    62581 atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt gtcagttcgc tggtggcggc
    62641 ttgttatttc ccttttgctc aggcctacgg cgcgcctaat ttcaatacat tgctggcatt
    62701 gcactccacc aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta
    62761 cctggtcggc ttatttattt ttgcgctcgg cgtaatagca atcaggcgaa aaaaagagaa
    62821 tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt
    62881 ttttgttgct cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg
    62941 ctatccggta tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga
    63001 gcaagaacgg atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa
    63061 gccgaagtat cagacctatg tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg
    63121 tgcctttggc ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt
    63181 tgctgactac attgccgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg
    63241 cgtagtcgat ggcaaaccac agtttcagga taactttgtc accctggcaa atcgcgcggg
    63301 cttccagacc tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ctgctatcgc
    63361 cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga
    63421 taaaaacacg aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca
    63481 ctcgcaaccg cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgacag
    63541 gacacaaggc aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct atctctatac
    63601 catgacgcaa acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag
    63661 cagcttctct ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga
    63721 cgtgcaatac cttgcccatg atgataaata tcagcaaaat ttccaggtgc cttttatggt
    63781 catttccagc gacgataaag cgcatcgggt gattaaagcc cgccgctcag ccaatgactt
    63841 cttaggcttt ttctcgcagt ggacgggaat taaagcgaag gaaataaata tcaaataccc
    63901 gtttatatct gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt
    63961 ggattacaac catctcggaa ccgatatttt cgacccgaaa ccttaaaaac aaaaatccgc
    64021 cccgagaggc ggatttttta tatcaccaaa gtgattagaa gcggtaacca acaccggcaa
    64081 tccaggtgcc tacgtcaacg ctacgaatac ggctctgctc gtaagagaag tccagagcaa
    64141 cgttttccat cgggttgaac tgcagaccag caccgtagga gaaaccgtag tcgctggtgt
    64201 cgtgtttgta ggtcgggtat tcagtggtct ggaatttacc ataacccaca cccactacac
    64261 cgtagatgct tgcccagtcg ttaatgcggt aagccggacc agcagtgatg ccgtagtact
    64321 ggtttttgtt gtagtcacca gagcttgcag tacggctttt ctcggtgtaa gtgaaagaac
    64381 cgatcacacc cagcgggctg ttgtcttctt catagcggta tttcaggttg aaaccgccca
    64441 ttttgttcat ttggccctga gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg
    64501 cagctacgga agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa
    64561 tttttttcat aaccacctca aatgtgattc aaataagtcc taagttttaa atatatcaaa
    64621 aattaatggg aaactcttcg cgatttgtga tgtctaacgg gccatttcat gtaacagaac
    64681 gtttccatac accgctatcc atctaaattt aaatcacttt ttcagagaac tgcgtaagta
    64741 ttacgcatgt tttccctgtc attcatccag attattccta atcaccagac taatgattcc
    64801 atcaatcctg gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca
    64861 gcattgggta tatccagtac actccacgct ttacttaagt ctagatattt gtgggagaaa
    64921 ggatgcctgg ttcattgcgt aaaatgccgg tctggttacc aatagtcata ttgctcgttg
    64981 ccatggcgtc tattcagggt ggagcctcgt tagctaagtc actttttcct ctggtgggcg
    65041 caccgggtgt cactgcgctg cgtctggcat taggaacgct gatcctcatc gcgttcttta
    65101 agccatggcg actgcgcttt gccaaagagc aacggttacc gctgttgttt tacggcgttt
    65161 cgctgggtgg gatgaattat cttttttatc tttctattca gacagtaccg ctgggtattg
    65221 cggtggcgct agagttcacc ggaccactgg cggtggcgct gttctcttct cgtcgcccgg
    65281 tagatttcgt ctgggttgtg ctggcggttc ttggtctgtg gttcctgcta ccgctggggc
    65341 aagacgtttc ccatgtcgat ttaaccggct gtgcgctggc tctgggggcc ggggcttgtt
    65401 gggctattta cattttaagt gggcaacgcg caggagcgga acatggccct gcgacggtgg
    65461 caattggttc gttgattgca gcgttaattt tcgtgccaat tggagcgctt caggctggtg
    65521 aagcactctg gcactggtcg gttattccat tgggtctggc ggtcgctatt ctctcgaccg
    65581 ctctgcctta ttcgctggaa atgattgccc tcacccgttt gccaacacgg acatttggta
    65641 cgctgatgag catggaaccg gcgctggctg ccgtttccgg gatgattttc ctcggagaaa
    65701 cactgacacc catacagctg ctggcgctcg gcgcaattat cgccgcttca atgggttcta
    65761 cgctgacagt acgcaaagag agcaaaataa aagaattaga cattaattaa attt
//
LOCUS       DAPHJE010000007        57924 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPHJE010000007 DAPHJE010000000
VERSION     DAPHJE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.007"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..2654
                     /gene="mrcB"
                     /locus_tag="RH222_000515"
     CDS             120..2654
                     /gene="mrcB"
                     /locus_tag="RH222_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV3795581.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQFSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2874..5063
                     /gene="fhuA"
                     /locus_tag="RH222_000516"
     CDS             2874..5063
                     /gene="fhuA"
                     /locus_tag="RH222_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV3795582.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5114..5911
                     /gene="fhuC"
                     /locus_tag="RH222_000517"
     CDS             5114..5911
                     /gene="fhuC"
                     /locus_tag="RH222_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV3795583.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5911..6801
                     /gene="fhuD"
                     /locus_tag="RH222_000518"
     CDS             5911..6801
                     /gene="fhuD"
                     /locus_tag="RH222_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV3795584.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6798..8780
                     /gene="fhuB"
                     /locus_tag="RH222_000519"
     CDS             6798..8780
                     /gene="fhuB"
                     /locus_tag="RH222_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV3795585.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8815..10095)
                     /gene="hemL"
                     /locus_tag="RH222_000520"
     CDS             complement(8815..10095)
                     /gene="hemL"
                     /locus_tag="RH222_000520"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV3795586.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10320..11741
                     /gene="clcA"
                     /locus_tag="RH222_000521"
     CDS             10320..11741
                     /gene="clcA"
                     /locus_tag="RH222_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV3795587.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11765..11830
                     /locus_tag="RH222_000522"
     CDS             11765..11830
                     /locus_tag="RH222_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795588.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11823..12167
                     /gene="erpA"
                     /locus_tag="RH222_000523"
     CDS             11823..12167
                     /gene="erpA"
                     /locus_tag="RH222_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV3795589.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12214..12837)
                     /locus_tag="RH222_000524"
     CDS             complement(12214..12837)
                     /locus_tag="RH222_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV3795590.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12875..13675)
                     /gene="btuF"
                     /locus_tag="RH222_000525"
     CDS             complement(12875..13675)
                     /gene="btuF"
                     /locus_tag="RH222_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV3795591.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13668..14366)
                     /gene="mtnN"
                     /locus_tag="RH222_000526"
     CDS             complement(13668..14366)
                     /gene="mtnN"
                     /locus_tag="RH222_000526"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV3795592.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14450..15967
                     /gene="dgt"
                     /locus_tag="RH222_000527"
     CDS             14450..15967
                     /gene="dgt"
                     /locus_tag="RH222_000527"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV3795593.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCYFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16097..17521
                     /gene="degP"
                     /locus_tag="RH222_000528"
     CDS             16097..17521
                     /gene="degP"
                     /locus_tag="RH222_000528"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV3795594.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            17676..18833
                     /gene="cdaR"
                     /locus_tag="RH222_000529"
     CDS             17676..18833
                     /gene="cdaR"
                     /locus_tag="RH222_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV3795595.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18922..19308)
                     /locus_tag="RH222_000530"
     CDS             complement(18922..19308)
                     /locus_tag="RH222_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV3795596.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19623..20447)
                     /gene="dapD"
                     /locus_tag="RH222_000531"
     CDS             complement(19623..20447)
                     /gene="dapD"
                     /locus_tag="RH222_000531"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV3795597.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20478..23150)
                     /gene="glnD"
                     /locus_tag="RH222_000532"
     CDS             complement(20478..23150)
                     /gene="glnD"
                     /locus_tag="RH222_000532"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV3795598.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23212..24006)
                     /gene="map"
                     /locus_tag="RH222_000533"
     CDS             complement(23212..24006)
                     /gene="map"
                     /locus_tag="RH222_000533"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV3795599.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24374..25099
                     /gene="rpsB"
                     /locus_tag="RH222_000534"
     CDS             24374..25099
                     /gene="rpsB"
                     /locus_tag="RH222_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV3795600.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25357..26208
                     /gene="tsf"
                     /locus_tag="RH222_000535"
     CDS             25357..26208
                     /gene="tsf"
                     /locus_tag="RH222_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV3795601.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26355..27080
                     /gene="pyrH"
                     /locus_tag="RH222_000536"
     CDS             26355..27080
                     /gene="pyrH"
                     /locus_tag="RH222_000536"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV3795602.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27230..27787
                     /gene="frr"
                     /locus_tag="RH222_000537"
     CDS             27230..27787
                     /gene="frr"
                     /locus_tag="RH222_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV3795603.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            27879..29075
                     /gene="ispC"
                     /locus_tag="RH222_000538"
     CDS             27879..29075
                     /gene="ispC"
                     /locus_tag="RH222_000538"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV3795604.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSCTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29264..30022
                     /gene="ispU"
                     /locus_tag="RH222_000539"
                     /gene_synonym="uppS"
     CDS             29264..30022
                     /gene="ispU"
                     /locus_tag="RH222_000539"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV3795605.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30035..30892
                     /gene="cdsA"
                     /locus_tag="RH222_000540"
     CDS             30035..30892
                     /gene="cdsA"
                     /locus_tag="RH222_000540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV3795606.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            30904..32256
                     /gene="rseP"
                     /locus_tag="RH222_000541"
     CDS             30904..32256
                     /gene="rseP"
                     /locus_tag="RH222_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV3795607.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32286..34718
                     /gene="bamA"
                     /locus_tag="RH222_000542"
     CDS             32286..34718
                     /gene="bamA"
                     /locus_tag="RH222_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV3795608.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            34840..35325
                     /gene="skp"
                     /locus_tag="RH222_000543"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             34840..35325
                     /gene="skp"
                     /locus_tag="RH222_000543"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV3795609.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35329..36354
                     /gene="lpxD"
                     /locus_tag="RH222_000544"
     CDS             35329..36354
                     /gene="lpxD"
                     /locus_tag="RH222_000544"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV3795610.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36459..36914
                     /gene="fabZ"
                     /locus_tag="RH222_000545"
     CDS             36459..36914
                     /gene="fabZ"
                     /locus_tag="RH222_000545"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV3795611.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            36918..37706
                     /gene="lpxA"
                     /locus_tag="RH222_000546"
     CDS             36918..37706
                     /gene="lpxA"
                     /locus_tag="RH222_000546"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV3795612.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37706..38854
                     /gene="lpxB"
                     /locus_tag="RH222_000547"
     CDS             37706..38854
                     /gene="lpxB"
                     /locus_tag="RH222_000547"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV3795613.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            38851..39447
                     /gene="rnhB"
                     /locus_tag="RH222_000548"
     CDS             38851..39447
                     /gene="rnhB"
                     /locus_tag="RH222_000548"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV3795614.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39482..42964
                     /gene="dnaE"
                     /locus_tag="RH222_000549"
     CDS             39482..42964
                     /gene="dnaE"
                     /locus_tag="RH222_000549"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV3795615.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            42977..43936
                     /gene="accA"
                     /locus_tag="RH222_000550"
     CDS             42977..43936
                     /gene="accA"
                     /locus_tag="RH222_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV3795616.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44035..46176
                     /gene="ldcC"
                     /locus_tag="RH222_000551"
     CDS             44035..46176
                     /gene="ldcC"
                     /locus_tag="RH222_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV3795617.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46233..46622
                     /locus_tag="RH222_000552"
     CDS             46233..46622
                     /locus_tag="RH222_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3795618.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46687..47985
                     /gene="tilS"
                     /locus_tag="RH222_000553"
     CDS             46687..47985
                     /gene="tilS"
                     /locus_tag="RH222_000553"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV3795619.1"
                     /translation="MTLTLNRQLLTLRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQKPQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(48034..48294)
                     /gene="rof"
                     /locus_tag="RH222_000554"
     CDS             complement(48034..48294)
                     /gene="rof"
                     /locus_tag="RH222_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV3795620.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSKS"
     gene            complement(48281..48481)
                     /locus_tag="RH222_000555"
     CDS             complement(48281..48481)
                     /locus_tag="RH222_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV3795621.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48647..49192
                     /locus_tag="RH222_000556"
     CDS             48647..49192
                     /locus_tag="RH222_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV3795622.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKFVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49189..49611
                     /gene="arfB"
                     /locus_tag="RH222_000557"
     CDS             49189..49611
                     /gene="arfB"
                     /locus_tag="RH222_000557"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV3795623.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49625..50335
                     /gene="nlpE"
                     /locus_tag="RH222_000558"
                     /gene_synonym="cutF"
     CDS             49625..50335
                     /gene="nlpE"
                     /locus_tag="RH222_000558"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV3795624.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(50490..51314)
                     /locus_tag="RH222_000559"
     CDS             complement(50490..51314)
                     /locus_tag="RH222_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDV3795625.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(51367..53085)
                     /gene="proS"
                     /locus_tag="RH222_000560"
     CDS             complement(51367..53085)
                     /gene="proS"
                     /locus_tag="RH222_000560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV3795626.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53196..53903)
                     /gene="tsaA"
                     /locus_tag="RH222_000561"
     CDS             complement(53196..53903)
                     /gene="tsaA"
                     /locus_tag="RH222_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV3795627.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLMAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(53900..54304)
                     /gene="rcsF"
                     /locus_tag="RH222_000562"
     CDS             complement(53900..54304)
                     /gene="rcsF"
                     /locus_tag="RH222_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV3795628.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54422..55237)
                     /gene="metQ"
                     /locus_tag="RH222_000563"
     CDS             complement(54422..55237)
                     /gene="metQ"
                     /locus_tag="RH222_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV3795629.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55277..55930)
                     /gene="metI"
                     /locus_tag="RH222_000564"
     CDS             complement(55277..55930)
                     /gene="metI"
                     /locus_tag="RH222_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV3795630.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(55923..56954)
                     /gene="metN"
                     /locus_tag="RH222_000565"
     CDS             complement(55923..56954)
                     /gene="metN"
                     /locus_tag="RH222_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV3795631.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57142..57714
                     /gene="gmhB"
                     /locus_tag="RH222_000566"
     CDS             57142..57714
                     /gene="gmhB"
                     /locus_tag="RH222_000566"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV3795632.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        13814 a        14271 c        15864 g        13975 t
ORIGIN      
        1 cctctccctc aagggagagg ggaccgatcg agcacaaatt ttgagagata tcttcttctg
       61 tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca
      121 tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac
      181 aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg
      241 aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgtg
      301 gcaaacgcgg ctggctatgg ctgctgttaa aactggctat cgtttttgcc gtgctgatcg
      361 ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc
      421 aactgcctgc ggcagtttat ggccgaatgg tcaaccttga gccagatatg accatcagca
      481 agaacgagat ggtgaagctg ctggaggcga ctcagtatcg tcaggtgtcg aaaatgaccc
      541 gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt ccgtttgatt
      601 tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggc gatcatctgg
      661 cgacgatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgtc
      721 tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt
      781 tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt tacgagcatg
      841 acggcatcag cctctactcc atcggacgtg cggtgctggc aaacctgacc gccggacgca
      901 cggtgcaggg agcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagtg
      961 agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt
     1021 acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg
     1081 acaacgaaat ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc ccggtagaag
     1141 agctaagcct cgaccagcag gcgctgttag tcggcatggt gaaaggagcg tccatctaca
     1201 acccgtggcg taacccaaaa ctggcgctgg agcgacgtaa tctggtgctg cgtctgctgc
     1261 aacagcaaca gattattgat caagaactct atgacatgtt gagtgcacgt ccgctgggtg
     1321 ttcagccacg cggtggggtg atctctccac agccagcctt tatgcaactg gtgcgtcagg
     1381 agctgcaggc gaaattaggc gataaggtaa aagatctctc cggcgtgaag atcttcacta
     1441 cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac
     1501 tgaagaaaca gcgtaagttg agcgatcttg aaactgcgat tgtggtcgtc gaccgcttta
     1561 gtggtgaagt tcgtgcgatg gtcggtggtt ctgagccgca gtttgcgggc tacaaccgtg
     1621 cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgactgcct
     1681 taagccagcc gaaaatctat cgtctgaata cgtggattgc ggatgcgcca atcgcgctgc
     1741 gtcagccgaa tggccaggtc tggtcaccgc agaatgatga ccgtcgttat agcgaaagcg
     1801 gcagagtgat gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg
     1861 ggatggcgct ggggctgcct gcggttacgg agacctggat caaactgggc gtaccgaaag
     1921 atcagttgca tccggttccg gcgatgctgc tgggggcgtt gaacttaacg ccaatcgaag
     1981 tggcgcaggc gttccagacc atcgccagcg gcggtaaccg tgcaccgctt tctgcactgc
     2041 gttcggtaat tgcggaagat ggcaaggtgc tgtatcagag cttcccgcag gcggaacgcg
     2101 ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgcg
     2161 gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga
     2221 ctaccaacaa taacgtagat acctggtttg cgggcattga cggcagcacg gtgaccatca
     2281 cctgggtagg ccgtgataac aaccagccga ccaaactgta tggtgccagc ggggcaatgt
     2341 cgatttatca gcgttatctg gctaaccaga cgccaacgcc gctgaatctt gttccgccag
     2401 aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc
     2461 gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagt gagatgcagc
     2521 agcaaccgtc aggcaatccg tttgatcagt tttctcagcc gcagcaacag ccgcaacagc
     2581 aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc aaggatatgt
     2641 ttggtagtaa ttaacatcta agcgtgaaat accggatggc gaattgccat ccggtaaaat
     2701 aacatcccac ctaaagtatt aaccctttct tttcatctgg ttgtttatta acccttcagg
     2761 aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt
     2821 ataataatca ttctcgttta cgttatcatt cactttacat cagagatata ccaatggcgc
     2881 gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag
     2941 cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca
     3001 ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg
     3061 cgaagcactc tgctactgcg actaaaacgg atacaccaat tgaaaaaacg ccgcagtcta
     3121 tttcggttgt gacaaatgaa gagatgcaga tgcatcaatt tcagtctgta aaagaagcat
     3181 taggttatac acctggcgtt acggttagca gtcgtggtgc ttctaataca tatgattttg
     3241 ttatcattcg tggtttctca tctgttggtc tgaatcaaaa taattacctt gatggactaa
     3301 aacttcaggg caacttttat aacgatgctg tgattgatcc ttacatgctc gagagggttg
     3361 aactgatgcg tgggccgacg tctgttctct atggaaaaag taatcctggt gggattattt
     3421 ctatggtgag taagcgtccg accactgaac ccctgaaaga aattcagttt aaaatgggga
     3481 cggataatct gtttcagact ggattcgatt tcagtgatgc actggatgat aacggtgagt
     3541 tctcctaccg tttgacgggc ctcgctcgtt caacaaacga acagcagaaa aattctgaat
     3601 cccagcgtta taccattgct ccatcattct catggcgtcc agacgacaaa accaatttta
     3661 ctttcctgtc ctatttccag aatgaaccag aaacgggtta ttacggttgg ttgccgaaag
     3721 aggggaccgt tgagccattg cctaatggta aacgtctacc gactgacttc aatgaaggtg
     3781 catcgaataa tacatactcc cgtaaccaga aaatggtggg atatagtttt gaacatggtt
     3841 ttaatgacac cttcaccgtg cgtcagaatc tgcgtttcag tgaaatgaaa acctcacaga
     3901 aaagtgttta tggcacaggg attgccaacg atggtcatac tctaaaccgc gggacagtgg
     3961 tggataatga gcgtctgcaa aactttagcg ttgataccca acttgaaagt aaatttgcta
     4021 caggtgaagt ggagcatact ttgctgacag gggtagactt catgcgtatg cgcaatgata
     4081 tcaatgccag ctttggatct gcaccatcaa tcgatcttta taacaaatat catcctgaat
     4141 actttgcatt tggtaacgca gagccatacc aaatgaatga aagcaaacaa acaggtattt
     4201 atgttcagga tcaggcggaa tggaataaat gggtattcac tctgggggga cgctacgatt
     4261 ggtctaagca agcgaccact gttcgtgaaa actcttatac gccgactgaa ggttatattg
     4321 agcgtaatga tcatcagttc acctggcgcg gtggtgtaaa ttacttattc gataatggta
     4381 tttcacctta ctttagctat agccagtcct ttgaaccgag tgctttcgat ctgtggagca
     4441 acccgcgcgt ttcctataag ccatcgaaag gtgaacagta tgaagctggc gtaaaatatg
     4501 ttccgaatga tatgccggtc gttgttacgg gcgcagtcta tcaattgacg aaaacaaata
     4561 acctgacagc agacccaaca aacccgttag cgcaagtccc agcaggtgag attcgcgctc
     4621 gtggtgtgga gcttgaagca aaagcagcgt taaatgccaa tattaacttg acggcttctt
     4681 atacctacac ggatgcggaa tacaccaaag acaccaatct caaaggtaaa actccagaac
     4741 aagtaccgga gcatatggca tctctctggg gggattatac cttcaatgaa gggccgcttt
     4801 ctggtttaac attgggaaca ggtggtcgtt ttattggttc cagctatggt gatccggcaa
     4861 acacttttaa agtgggtagc gcagctgtaa tggatgctgt tgtaaaatat gatctggcac
     4921 gctttggtat ggcgggatcc agccttgctg tgaacgtcaa caatttgctc gatcgtgagt
     4981 atgttgccag ttgcttccag acttatggct gcttctgggg cgcagaacgt caggtcgttg
     5041 caaccgcaac cttccgtttc taatttctct tttggggcac ggattaccgt gcccatttca
     5101 caagttggct gttatgcagg aatacacgaa tcattccgat accacttttg cactgcgtaa
     5161 tatctccttt cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc
     5221 cgggaaagtg accggtctga tcggtcacaa cggttctggt aaatccactc tgctcaaaat
     5281 gcttggccgt catcagcctc cgacggaagg ggagattctt cttgataccc agccgctgga
     5341 aagctggagc agcaaagcgt tcgcccgcaa agtggcttat ttgccgcagc agcttcctcc
     5401 ggcagaaggg ttgaccgtgc gtgaactggt ggcgattggt cgttacccgt ggcatggcgc
     5461 gctggggcgc tttggggcgg cagatcgcga aaaagtcgag gaagctatct cgctggttgg
     5521 cttaaaaccg ctggcgcatc ggctggtcga tagtctctct ggcggcgaac gtcaacgggc
     5581 gtggatcgcc atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc
     5641 ggcgctggat atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt taagtcagga
     5701 acgtggcctg acggtcattg ccgtgctgca cgatatcaat atggcggcac gctactgtga
     5761 ttatctggtc gccctgcgcg gtggtgaaat gattgctcag ggaacgcctg cggaaattat
     5821 gcgcggcgaa accctcgaaa tgatttatgg catcccgatg ggtattttgc cgcatccggc
     5881 gggtgctgca cctgtgagtt ttgtttattg atgagcggct tacctcttat ttcgcgccgt
     5941 cgactgttaa cggcgatggc gctttctccg ttgttatggc agatgaatac cgcccacgcg
     6001 gcggctattg accccaatcg tattgtggcg ctggagtggt tgccggtgga attactgctg
     6061 gcgctcggca tcgtgcctta cggcgtggcg gataccatca actatcgcct gtgggtcagc
     6121 gaaccaccat tgccggactc agtgattgac gtcggtttgc gtacagaacc taaccttgaa
     6181 ctgctgaccg aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg cccttcatca
     6241 gaaatgctgg cacgtattgc gccgggtcgc ggatttaact tcagtgacgg caaacagccg
     6301 ttggcgatgg cgcgtaaatc gctgacggaa atggcagatt tacttaacct gcaaagcgca
     6361 gcggaaacgc atttagcgca atacgaagac tttatccgca gcatgaaacc ccgctttgtg
     6421 aagcgtggtg cacgcccgtt actgctgacg acgcttatcg atccgcgcca tatgctggtc
     6481 ttcggtccaa acagcttgtt ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg
     6541 caaggggaaa ccaacttctg gggcagtacc gccgtcagta tcgatcgtct ggcggcgtat
     6601 aaagacgttg atgtgctctg ttttgatcac gacaacagca aagacatgga tgcgctaatg
     6661 gcaaccccgc tgtggcaggc catgccgttt gtccgtacag ggcgctttca gcgcgtaccc
     6721 gcagtctggt tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt tctggataac
     6781 gccatcggag gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc
     6841 tgttagtgat tgtcgctacg gcgctaacct ggatgaactt ctcacaggcg ctgccgcgca
     6901 gtcagtgggc gcaggccgcc tggtcgccgg atattgacgt catcgagcag atgatttttc
     6961 actacagctt gttgccgcgt ctggcgattt cgctgctggt gggcgcaggc ctggggctgg
     7021 tgggcgtgct gtttcagcaa gtgctgcgta atccgctggc ggaaccgacg acacttggcg
     7081 ttgctacagg cgcgcaactg ggaattaccg tcaccacact ttgggcgatc ccaggtgcta
     7141 tggcgagcca gtttgccgca ctggtgggcg cttgtgtcgt tggcttaatc gtctttggcg
     7201 tcgcgtgggg gaaacggctt tcgccggtta cgcttattct cgcggggctg gtggtgagcc
     7261 tttattgcgg ggcaattaat cagctactgg ttatcttcca tcacgaccag ctgcaaagca
     7321 tgttcctgtg gagcaccgga acgctgacgc aaaccgactg gggcggcgtt gagcgtttat
     7381 ggccgcagtt gctgggcggc gtgatgttga cgttattgct acttcgcccg ttaaccctga
     7441 tggggcttga tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctc gcgcgtctgg
     7501 cagcgctgtc gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg gggattatcg
     7561 gctttatcgg gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc
     7621 cacgactgat gctggcgtcg ctgattgggg cgctgatcct ctggctttcc gatcaaatca
     7681 tcctctggct gactcgcgtg tggatggaag tgtccaccgg ttcggtcact gcgttgatcg
     7741 gtgcgccgct gctactgtgg ctgttgccgc gtttacgcag cattagcgcg ccggatatga
     7801 aggtcaacga tcgtgtcgcg gctgaacgcc aacatgtgct ggcgtttgcc cttgctggcg
     7861 gtgtgctgct gataattgct gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct
     7921 ggacgtgggc gagcggggcg ctgctcgatg atttaatgcc ctggcgctgg ccgcgaatta
     7981 tggcggcgct gtttgcgggc gtcatgctgg cggtggcggg ctgtattatt cagcggctga
     8041 ccggaaaccc gatggcaagc ccggaagtgc tggggattag ctctggcgca gcgtttggcg
     8101 tggtgttgat gctgtttctg gtgccgggta atgcctttgg ctggttgtta cctgcaggca
     8161 gtctcggggc ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc ggtggatttt
     8221 ccccacaccg catgttactg gcggggatgg cgttaagtac cgcgttcacc atgcttttga
     8281 tgatgttgca ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg atttccggtt
     8341 cgacctacaa cgcgaccgat gcgcaggtct ggcgcaccgg aattgtgatg gtgattttgc
     8401 tggcgattac cccgctgtgc cgccgctggc tgaccatttt accgctgggt ggtgataccg
     8461 cccgagccgt aggaatggcg ctaacgccga cgcgaattgc gctgctgctg ttagcggctt
     8521 gcctgacggc gaccgcgacg atgactattg gaccgttgag ttttgttggt ttaatggcac
     8581 cgcatattgc gcggatgatg ggctttcgac ggacgatgcc gcacatcgta atttcggcgc
     8641 tggtgggtgg tttactgctg gtgttcgctg actggtgtgg gcggatggtg ctgtttccat
     8701 tccagatccc ggcggggctg ctgtcaacct ttatcggcgc gccatatttt atctatttgt
     8761 tgagaaagca gagccgttaa ttgaatgcct cgcccggtgg gcgaggcaaa atcatcacaa
     8821 cttcgcaaac acccgacgtg cagcatcgat ggtgttattg atatcttcca tgctgtgcgc
     8881 cacggacata aagcccgctt caaacgctga cggtgccagg taaacacctt catccagcat
     8941 catatggaag aaacgcttaa agcgttccac gtcacaggcc atcacatcct gatagcacgt
     9001 cacggactcg gcgtcggtaa agaaaatacc gaacatgccg ccaacgtggt taacgaccag
     9061 cggaattccg gcttcttctg ccgcttccag cagaccttct gccagacgtg atgtcagctc
     9121 atccaacgtt tcgtgaacgc ccggctgcgc gacttcattc agacaggcga aaccggctgc
     9181 catcgcaatt gggttaccgg aaagcgtacc cgcctgatag accggacccg tcggggccag
     9241 cgcatccatt acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat
     9301 ttttccgagg caggttaaat ctggcactac gccgtaataa tcctgtgcac cagccagtgc
     9361 cacgcggaag ccagtcatta cttcatcgat gatcagcaat gcgccaaatt cgtcgcacag
     9421 cgcgcgcaga cctggcagga actctggcag cggcggaaca cagttcatat tgcctgccac
     9481 cggctcaacg ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga
     9541 agccagatcg ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacacccgg
     9601 cgagtttggc tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc
     9661 gtgaccgtgg taacaacctt caaatttaat gattttgtcg cgaccggtaa aaccacgggc
     9721 caggcggatg gcgctcatgg tcgcttcagt gccggagttc accatgcgca ccatatccat
     9781 ggtcgggacc agttcggtca ccagttgcgc cattttcact tccatttcgg ttggtgcacc
     9841 aaagcttaaa ccacgctcgg cggcttcaat cacggcattg cggattgccg gatggttatg
     9901 gcccagcacc atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc
     9961 gtacagataa gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa
    10021 ggcgcgaaca ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt
    10081 ttcagactta ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattctat
    10141 gttattcata acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct
    10201 gatttcttag agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc
    10261 atggaacgtg cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga
    10321 tgaaaactga tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac
    10381 tgattcgcca acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag
    10441 tcgtcggcac gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc
    10501 agaaccaacg tatgggggcg ctggtacata ctgctgataa ttatccgcta ctgttaactg
    10561 tcgcttttct ctgttcggcg gtgctggcga tgttcggcta ctttttggtg cgcaaatacg
    10621 cgcccgaagc agggggatcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc
    10681 ctgttcgctg gtggcgtgta ttgccggtga aattctttgg cgggctgggg acactcggcg
    10741 gaggcatggt gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat atcggtcgca
    10801 tggtgcttga tattttccgc ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg
    10861 gtgctgctgc ggggctggct gcggccttta acgcgccgct ggcgggtatt ttgtttatta
    10921 tcgaagagat gcgtccgcag tttcgctata cgttaatttc gattaaagcg gtatttattg
    10981 gcgtcattat gtcgaccatt atgtaccgga tttttaatca tgaagttgcg ttgattgacg
    11041 tcggtaaact ttctgatgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta
    11101 tttttggcat tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc
    11161 accgtgtgca cggcggcaat attaccaaat gggtgctaat gggcggtgcg attggcggtc
    11221 tgtgtggatt gctggggttt gtggcaccag caacgtcggg cggcggtttt aacctgattc
    11281 ctatcgctac cgcggggaat ttcagcatgg gaatgctggt gtttatcttc gtcgcgcggg
    11341 tcattaccac cttactctgc ttctcttccg gcgcgccggg cggtattttt gccccgatgc
    11401 tggcgctggg tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc
    11461 aatatcacct tgaggcggga acgtttgcta ttgccggaat gggggcgtta ctggcggcat
    11521 ctattcgcgc gccgttaacg gggatcattc tggttctgga gatgaccgat aactaccagc
    11581 tcattttgcc aatgattatt accggtcttg gcgcaacact attagcgcaa tttaccggcg
    11641 ggaaaccgct atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctgagcaac
    11701 tggcgcgaag caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta
    11761 gataatggcg attattattg ggttagaatt tgcccaattg ccgatgtcgt ttggagcaaa
    11821 atatgagtga tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta
    11881 aaagcctgat cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcaccggtg
    11941 gcggttgcag cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata
    12001 tgaccatcga aaaacagggc gttggcctgg tggttgatcc gatgagcctg caatatctgg
    12061 tcggcggttc cgttgactat accgaaggac tggaaggttc tcgtttcatc gtgaccaacc
    12121 cgaacgcgaa aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg
    12181 tgccggataa actatccggc acatcttctc tcttcaacgc ccattctcat ccagcgcaaa
    12241 cgtcggtagc ttaagatgcc aacgaatagc cgccagccga atcaatagcg tcacgaccat
    12301 gcccatcata ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac
    12361 aataccgccg ataatacagg cagttgcgta gatttctgta cgtaaaatca tggggatctc
    12421 gcgggccagt acatcacgaa taatcccgcc gccaacgcca gtaatgacgc ccatacaaac
    12481 cgcgattaac ggaccggctt ccgcattaaa ggctttattc acgccaatgc cgacaaacac
    12541 cgccagacca acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg
    12601 caccagcacg atggtcagca tgctggtgac cattgcaacg accagatcgg tgggatcttt
    12661 cacccaaaat accgggccgt gatccagcgc catgtcgcga attgttccgc cgcctactgc
    12721 ggttaccacg cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa
    12781 aacgccggag atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt
    12841 tcaatcccca ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa agcgcattac
    12901 agagctgttg tgctgcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga
    12961 gaggaataac gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt
    13021 ccggtccgcc tgtaatgaca atcgcctgtg gcgagcgtgc taacacctgt tcgcggctaa
    13081 cttgcggcca gggaacccgg ctgtctttaa agatgttttc tccgccacaa acttcgagta
    13141 cctggttctg aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa
    13201 aaacacgttt tttaggttta tcagcatatt gcgctttcaa ttgcgcgtac tgatccagca
    13261 gggattgcgc ggcttgttcg gccttgtctg gttgcggact ccagggggcc agttgacgta
    13321 acgcattggc aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg
    13381 aagccagctg gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg
    13441 gcttcagcgc gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct
    13501 tttgcgcttg tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcag
    13561 caaaggcaag ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc
    13621 acagtggcgc aagaaaagac agggcgacca gcgccctgaa cagtgactta gccatgtgca
    13681 agtttctgca ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac
    13741 tcatcgaagc taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca
    13801 aacgggacgt tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca
    13861 atggcctgtg ggaagttgtg gcggattttc gccagaccaa cagaaccgtt gatgaaagcg
    13921 tcgccgctaa caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca
    13981 gcggcagcga tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg
    14041 tattcataac caaatgccgt gacatccgcg tcgtgataac gtgcttcgtc cgagacaacg
    14101 atatcgccca ctttcaacgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca
    14161 tctggcttgc agtgttccaa cagcaaagtg gcacccagcg ccgcagcgac tttaccgatg
    14221 cccgatttca gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg
    14281 ccgagactga tagtttgacg tttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc
    14341 attgcaccaa tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaggcat
    14401 agtttaccat gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat
    14461 tgatttccga aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac
    14521 cgaacatgag atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc
    14581 aattcgtcgt ctgcaacaaa agacccaggt ttttccactg gagcgcaatg ccgccgtgcg
    14641 cacgcgtctt acccactcga tggaagtcca gcaggtgggg cgctacatcg ccaaagaaat
    14701 tctaagccgt ctgaaagagc ttaaattact ggaagcatac ggcctggatg aactgaccgg
    14761 cccctttgaa agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt
    14821 tggtcatttt ggcgaagcgg cgataaatga ctggtttcgc cagcgtttat acccggaaga
    14881 tgccgaaagc cagcctctga ctgacgatcg ctgcagcgtg gcagcactac gtttacggga
    14941 cggggaagaa ccgcttaacg agctgcggcg caagattcgt caggacttat gttattttga
    15001 ggggaatgca caaggcattc gtctggtgca tacattgatg cggatgaatc tcacctgggc
    15061 acaggttggc ggtattttaa aatatacccg tccggcgtgg tggcgtggcg aaacgcctga
    15121 gacacatcac tatttaatga aaaagccggg ttattatctt tctgaagaag cctatattgc
    15181 ccggttgcgt aaagaactta atttggcgct ttacagtcgt tttccattaa cgtggattat
    15241 ggaagctgcc gacgatatct cctattgtgt ggcagacctt gaagatgcag tagagaaaag
    15301 aatatttacc gttgagcagc tttatcatca tttgcacgaa gcgtggggcc agcatgagaa
    15361 aggttcgctc ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct caaatagttt
    15421 aagccgcagt acggaagatc agttttttat gtatttacgg gtaaacaccc taaataaact
    15481 ggtaccctac gcggcacaac gatttattga taatctgcct gcgattttcg ccggaacgtt
    15541 taatcatgca ttattggaag atgccagcga atgcagcgat cttcttaagc tatataaaaa
    15601 tgtcgctgta aaacatgtgt ttagccatcc agatgtcgag cagcttgaat tgcagggcta
    15661 tcgggtcatt agcggattat tagagattta tcgtccttta ttaagcctgt cgttatcaga
    15721 ctttactgaa ctggtagaaa aagaacgggt gaaacgtttc cctattgaat cgcgcttatt
    15781 ccacaaactc tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta aattaccgtc
    15841 agattctcct gagtttccgc tatgggaata ttattaccgt tgccgcctgt tgcaggatta
    15901 tatcagcggt atgaccgacc tctatgcgtg ggatgaatac cgacgtctga tggccgtaga
    15961 acaataacca ggcttttgta aagacgaaca ataaattttt accttttgca gaaactttag
    16021 ttcggaactt cgcgctataa aacgaatctg aagaacacag caattttgcg ttatctgtta
    16081 atcgagactg aaatacatga aaaaaaccac attagcactg agtgcactgg ctctgagttt
    16141 aggtttggcg ttatctccgc tctctgcaac ggcggctgag acttcttcag caacgacagc
    16201 ccagcagatg ccaagccttg caccgatgct cgaaaaggtg atgccttcag tggtcagcat
    16261 taacgtagaa ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt
    16321 cttcggtgat gattctccgt tctgccagga aggttcaccg ttccagagct ctccgttctg
    16381 ccaggggggc cagggcggta atggcggcgg ccagcaacag aaattcatgg cgctgggttc
    16441 cggcgtcatc attgatgccg ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa
    16501 cgcgacggtg attaaagtcc agctgagcga tggtcgtaag ttcgacgcga agatggttgg
    16561 caaagatccg cgctctgata tcgcgctgat tcagatccag aacccgaaaa acctgaccgc
    16621 aattaagatg gcggattctg atgcactgcg cgtgggtgat tacaccgtag cgattggtaa
    16681 cccgtttggc ctgggagaga cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg
    16741 cctgaatgcc gaaaactacg aaaacttcat ccagaccgat gcagcgatca accgtggtaa
    16801 ctccggtggt gcgctggtta acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct
    16861 cgcaccggac ggcggcaaca tcggtatcgg ttttgctatc ccgagcaaca tggtgaaaaa
    16921 cctgacctcg cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg gtattatggg
    16981 gactgagctg aattccgaac tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt
    17041 cgtaagccag gttctgccta attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt
    17101 gatcacctca ctgaacggta agccaatcag cagctttgcc gcactgcgtg ctcaggtggg
    17161 tactatgccg gtgggcagca aactgaccct gggcttgctg cgcgacggta agcaggtcaa
    17221 cgtgaatctg gaactgcagc agagcagcca gaatcaggtt gattccagct ccatcttcaa
    17281 cggcattgaa ggtgctgaga tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa
    17341 cgtgaaaacg ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg tgattattgg
    17401 cgcgaaccag caggcagtga aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc
    17461 gtctgtgctg gcactgaaca ttcagcgcgg cgacagctcc atctacctgt taatgcagta
    17521 atctccctca accccttcct gaaaacggga aggggttctc cttacaatct gtgaacttca
    17581 ccacaactcc atacatcttc atcatccttt aggcatttgc acaatgccgt acgttacgta
    17641 cttccttatg ctaagccgtg cataacggag gacttatggc tggctggcat cttgatacca
    17701 aaatggcgca ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa
    17761 tggatgcccg tgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg
    17821 aaggtgcatt gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtggcac
    17881 gtcatctgca cggtgtgcgg caagggatta atctaccgtt acggctggaa ggtgaaattg
    17941 tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg taaatatggc gaactggtct
    18001 gcatgacggc tgaaatgatg ctggaacagt cgcggttgat gcacttgttg gctcaggata
    18061 gccgtttgcg ggaggaactg gtgatgaacc tgattcaggc agaggagaat actcccgcac
    18121 ttactgaatg ggcgcaacgg ctggggatcg atctcaatca accgcgagtg gtggctattg
    18181 ttgaggtcga cagcggtcag cttggtgtgg acagcgcaat ggcggagtta caacaactgc
    18241 aaaacgcgct gactacgccc gagcgtaata atctggtggc gattgtctcg ctaaccgaaa
    18301 tggtggtgtt gaaaccggcg ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta
    18361 agcgagttga acaactgatt acccgcatga aagagtacgg ccagctgcgt tttcgcgttt
    18421 cactgggcaa ctattttacc ggtcctggca gtattgcccg atcctatcgt acggcgaaaa
    18481 cgacgatggt ggtgggtaaa cagcggatgc cagaaagtcg ctgctatttt tatcaggatc
    18541 tgatgttacc tgtgttactc gacagtttgc gtggcgactg gcaggccaac gaactggcgc
    18601 gaccgctggc gcggctgaaa gcgatggaca ataacggctt gctgcgacga acgctggcgg
    18661 cgtggtttcg tcacaatgtg caaccgctgg caacgtcaaa ggcgttgttt attcatcgta
    18721 atactctgga gtatcggctt aatcgtatat cggaactgac cgggcttgat ttgggtaatt
    18781 ttgatgacag gttgctgctg tatgtggcgt tgcagctgga tgaagagcgg taggttatgc
    18841 gttaaggtcg aggcggcgct ggctcattcc cctcagcccg aagggtagag gggaatgtcc
    18901 gagttaaccg tcagttcggc attatttacg cgttagtttt tccagatcgg cttcaatctc
    18961 gctgatctta ttggttacga ctgactccag gtgacgtaag tcgtcgagga tcttacgctt
    19021 aagatcaact tcgctgcggt cacgctggca gatttgatca agctcatcaa tgatataccg
    19081 tagattcggg ctgatttcct ggacttcttt ataaccctga cccacaccat cagcgacgac
    19141 cgttttacgc tgacgcggat atttaaactt aacgctcttg gcgaaaaact cgcctttgtc
    19201 tttctggaaa tagattttca gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc
    19261 aatttcttca ggattggtaa tacccagact tttcagattg tcgtacatag cgttacctca
    19321 aaatgagtca gtaaattgtg cttattttag catttggcct ggcccgcccg gctcgactgt
    19381 gatcgcagaa agcctttttc ttccagactt ctatcatggc gtaaaaatca aaaaattacc
    19441 tgctttattg tggcgatatt ccatgggatg gattaacaca tttatcttcg ttttgcgaat
    19501 tacatgacag tccgaaacaa gatgaaaaag tatttaaaaa aggggggaag aagaatgagt
    19561 aggacgaatt attctgagcc agaaagcata cgggcagcat catgctgccc gtatatgtac
    19621 tcttaatcga tggtacgcag cagttcgtta atgccgactt tgccgcgagt tttcgcgtca
    19681 actttcttaa cgataaccgc acagtagagg ctgtatttgc catctttcga cggcagatta
    19741 cctgaaacaa ccacagaccc cgccggaacg cgaccgtagt ggatttcgcc ggtttcacgg
    19801 tcgtaaatac gggtgctctg accaatgtat acgcccatag agatgacgga accttcttcg
    19861 acaatcaccc cttcaaccac ttcggagcgt gcgccaatga agcagttatc ttcaataatg
    19921 gtcgggttag cctgcagcgg ctccagcacg ccgccgatgc ccacgccacc ggaaaggtgg
    19981 acgtttttac caatctgcgc acaagaaccg acggtcgccc aggtatcaac catcgtgcct
    20041 tcatcaacat atgcgccgat gttgacgtaa gacggcatca gcacggtgtt acgggcaata
    20101 aatgcaccct gacgtaccgc cgctggtggc acaacgcgga agccttcttt ctgaaaacgc
    20161 gcttcgtcgt agtcggcgaa tttcatcggc actttatcga agtagcggct ttctgcccct
    20221 tcgatcacct gattatcatt aatacggaaa gagagcagca ccgctttttt caaccactga
    20281 tgcgtcaccc actgaccgtc aattttttcc gctacacgca gtgcgccgga atccagcagg
    20341 gcgatcacct gatttaccgc ttcgcgggtt acggtgtctg cattggctgg cgtgatctcg
    20401 gcacggcgtt caaaagcggt ttcaataatg ttctgtaact gctgcattgt taaactcttt
    20461 tcatatcagt aaacacatca ccctttatcg tttggattga gggcctctgt caaccgctga
    20521 tgcacttcct gctgcaactc gttattaagt gcacgccggt cagcggtggc aattatgaat
    20581 aaatcttcta ctcgctcgcc aatggttgta attcgggcac catgaagcga aattcccaga
    20641 tcggcaaaaa ttttcccgac tctcgccagc agtcctggct ggtcgagcgc gataagctca
    20701 aggaacgatt tgcggtcggt atgggtcggc aaaaacgtta cttcggtttc aacagtaaaa
    20761 tggcgtaatt tggcgggttg gcgacggggc tgcggtggct gccagctact ttgcgtcagt
    20821 acttgctcca gaccaaaccg aataacctca tgacgatctg cggacagcgg gctgccatcg
    20881 ggttccagca cgataaaggt atccatcgcc ataccgtcgc gagtggtgaa aatttgtgcg
    20941 tcgtgaacac ttaaattgcg gcggtctaat tcggcacaga cggcggcaaa cagatagggg
    21001 cggtccgggc tccagataaa aatctcggtg cctccacgcg tagcctgcgg gctaagcaat
    21061 accagcggtt tgcttaaatc atgctgtaat aaatggcggg catgccaggc cagttgattt
    21121 gggctatggc ggacaaaata gttagcacga cagcgtgacc aaatttggtg cagcgcctct
    21181 tcgtcgatgt tatccatgcg cagtagtgcc agtgcctgga gttggtgatg gcgaacccgt
    21241 tcgcgcatat ccggcgtgtt ttgcattccg cgtcgtagct gcttttcggt ggcaaaatag
    21301 agttcgcgca acagactttg cttccagcta ttccacagcg tttcgttggt ggcgcaaatg
    21361 tcagccactg tcaggcatac cagatagcgc agacgatttt ccgtttgaac ttcttcggca
    21421 aactgcttga tgacttcagg gtcctgaata tcgcggcgtt gtgctgtcac cgacatcagc
    21481 aagtgctggc gaaccagcca ggcaaccagc tgtgtttcgc gtgagttcag cccgtggagt
    21541 tctgcaaaat gcactacatc ctgagcaccg agaatggagt ggtcgccgcc gcgtcctttg
    21601 gcgatatcgt gaaacagcgc ggcgatgaaa atcagctcag ttgacggcag gcgcggccag
    21661 acgtccacac acaacggatg gcgctggcgc gtttcttcac tggcaaaact ctccagtttc
    21721 agcatcacgc ggatagtatg ttcatccacc gtgtaggcgt ggaacagatc aaactgcatc
    21781 tgcccgacga tatgcgacca ttgcggcata tacgcgccga gcacgctatg gcgatgcatt
    21841 ggcaatagcc cgcgccgcac cgctccgggg tgacgcagaa tgctcaaaaa cagttttcgt
    21901 gcttccggaa tattacacag cggttgttgc agatggcgac gggcatggcg taactggcgc
    21961 agcgtggtgg agtaaatgcc ggtgatcgca ctgttgcgca ccatggtgta gaacatacgc
    22021 aagatggctt ccggctggcg cataaatagt gtttcatcac gcaggtcgat tagcgtaccg
    22081 cgtagctgaa actcatcgtc gattggacgt ggtttttcgt cggcgggaag ggcgaggatg
    22141 gcttcatcga acagttgcag cagcatctgg ttgagttcac tgacgcggcg tgtaacgcgg
    22201 aagtaatcct tcatcatccg ctcgaccggt tcgttgcctt caccactgta attcagacgc
    22261 tgagcgacgc taagttggcg atcgaataac agacgattat cgtaacggct gacgaccaga
    22321 tgcagggcaa agcgaatacg ccacagtata tgcagacatt cgtttaattc cgcccgctcc
    22381 gctgaggtta agaagccaaa cccgaccatt tcatccagcg atgttgcgcc aaaatgacgg
    22441 cgggccaccc attgcagagt gtgaatatcg cgcaagccgc cagggctact tttgatgtct
    22501 ggttcaaggt tgtagctggt gccatggtaa cgctgatggc gctggttctg ttcttcaact
    22561 ttcgccgcgt agaacttgtc ggaaggccag aatccttcgc taaaaatatg tttttgcagt
    22621 tcgaggaaga gcgcaacatc gccaattaat aagcgggatt cgattaaatt ggtggcgacg
    22681 gttaaatccg ataacccttc cagcatgcac tcttcaagcg tgcgcacact atgaccgact
    22741 tccagcttta catcccagag cagcgttaac agctcgccca ctttttgcgc ctgatcgtcc
    22801 gggagctttt tacggcttaa aatcagtaaa tcgatgtcag aaagtggatg cagctcgcca
    22861 cgaccgtagc caccgacggc gaccaatgcc aggtcggcaa tctggctgaa tcccgcttca
    22921 atccataatc gttgcaggag ctggtcgata aactcggtgc gcgcctcaat caactgttct
    22981 gcagagatcc cattgtcaaa ggcatcaccc agccaacgct ggaaagtatc gatatgggct
    23041 tttatcccac cgacggttaa ttcatcacgg ggccagacgc atggattttg cggttgaccg
    23101 ggcagggtgg ggagagcggt gtttgcgtac tgttctggaa gggtattcat tgtgcgccac
    23161 ccataagatt aaattatcgc attaaaaaag ccggcattcg ccggcttcat cttattcgtc
    23221 gtgcgagatt atcgccggga tggtgtcatc cttgcgtagc gtcaaaattt cgcagccgtt
    23281 atcagtcacc acaatagtat gctcatattg tgcagacaag ctgcgatctt tggtttttac
    23341 cgtccagcca tctttcatgg tgcggatctc ttttttaccc gcgttgacca ttggctcaat
    23401 ggtgaacgtc atccccggtt tcagcacgac gttggtttca cgggagtcat agtgcagcac
    23461 ctgcggttct tcatggaagc cgcgaccaat accgtgtcca caatattcac gaacgacgga
    23521 gaagccttct gcttcgacaa acttctgaat cgccgcaccg atttcgcgca ggttaatgcc
    23581 tggtttgacc atgcgcagcg ccaggtacag gctttcttgc gtgatgcggc acagacgttc
    23641 gcccatgatg gtcggcttac cgacgataaa cattttcgag gtatcgccgt ggaaaccatc
    23701 tttgattacg gtgacatcaa tgttaacgat atcgccatct ttcagcagct tagcatcgtc
    23761 cgggataccg tggcacacca cttcattaat agagatgcaa acggatttcg gatagccgtg
    23821 atagccgagg caggcagaaa ccgcgtgttg ttcattaaca atgtaatcat tacagatgcg
    23881 atccagctcg ccggtgctga cgcccggttt aacatacggt tcgatcatct ccagcacttc
    23941 ggcagccagt cggccagcga cgcgcatttt ttcgatatct tctggggtct tgattgagat
    24001 agccattaat tctgtccatc agcgtcggtg ataccgacaa tatatatgta agtgccgtca
    24061 atggtatcac acccgggcaa attgagaatc attctgaatt tcgccaaacg tgccactgaa
    24121 ggttttctat aatagaaaat tcgacatctg atgctgtaca cagcgccaac aattattggt
    24181 gtccatgacg tatttgtggt ataaagcgcg ccggacttcc gatccatttc gtatacacag
    24241 actggacgga agcgacaatc tcactttgtg taacaacaca cacgtatcgg cacatattcc
    24301 ggggtgccct ttggggtcgg taatatggga tacgtggagg cataacccca acttttatat
    24361 agaggtttta atcatggcaa ctgtttccat gcgcgacatg ctcaaggctg gtgttcactt
    24421 cggtcaccag acccgttact ggaacccgaa aatgaagccg ttcatcttcg gtgcgcgtaa
    24481 caaagttcac atcatcaacc ttgagaaaac tgtaccgatg ttcaacgaag ctctggctga
    24541 actgaacaag attgcttctc gcaaaggtaa aatccttttc gttggtacta aacgcgctgc
    24601 aagcgaagcg gtgaaagacg ctgctctgag ctgcgaccag ttcttcgtga accatcgctg
    24661 gctgggcggt atgctgacta actggaaaac cgttcgtcag tccatcaaac gtctgaaaga
    24721 cctggaaact cagtctcagg acggtacttt cgaaaagctg accaagaaag aagcgctgat
    24781 gcgcactcgt gagctggaga aactggaaaa cagcctgggc ggtatcaaag acatgggcgg
    24841 tctgccggac gctctgtttg taatcgatgc tgaccacgaa cacattgcta tcaaagaagc
    24901 aaacaacctg ggtattccgg tatttgctat cgttgatacc aactctgatc cggacggtgt
    24961 tgacttcgtt atcccgggta acgacgacgc aatccgtgct gtgaccctgt acctgggcgc
    25021 tgttgctgca accgtacgtg aaggccgttc tcaggatctg gcttcccagg cggaagaaag
    25081 cttcgtagaa gctgagtaat aaggcttgat aactccccca aaatagttcg agttgcagaa
    25141 aggcggcaag ctcgagaatt cccgggagct tacatcagta agtgaccggg atgagcgagc
    25201 gaagataacg catctgcggc gcgaaatatg aagggggaga gcccttatag accaggtagt
    25261 acacgtttgg ttagggggcc tgcatatggc cccctttttc acttttatat ctgtgcggtt
    25321 taatgccggg cagatcacat ctccgaggat tttagaatgg ctgaaattac cgcatccctg
    25381 gtaaaagagc tgcgtgagcg tactggcgca ggcatgatgg attgcaaaaa agcactgact
    25441 gaagctaacg gcgacatcga gctggcaatc gaaaacatgc gtaagtccgg tgctattaaa
    25501 gcagcgaaaa aagcaggcaa cgttgctgct gacggcgtga tcaaaaccaa aatcgacggc
    25561 aactacggca tcattctgga agttaactgc cagactgact tcgttgcaaa agacgctggt
    25621 ttccaggcgt tcgcagacaa agttctggac gcagctgttg ctggcaaaat cactgacgtt
    25681 gaagttctga aagcacagtt cgaagaagaa cgtgttgcgc tggtagcgaa aattggtgaa
    25741 aacatcaaca ttcgccgcgt tgctgcgctg gaaggcgacg ttctgggttc ttatcagcac
    25801 ggtgcgcgta tcggcgttct ggttgctgct aaaggcgctg acgaagagct ggttaaacac
    25861 atcgctatgc acgttgctgc aagcaagcca gaattcatca aaccagaaga cgtatccgct
    25921 gaagtggtag aaaaagaata ccaggtacag ctggatatcg cgatgcagtc tggtaagccg
    25981 aaagaaatcg cagagaaaat ggttgaaggc cgcatgaaga aattcaccgg cgaagtttct
    26041 ctgaccggtc agccgttcgt tatggaacca agcaaaactg ttggtcagct gctgaaagag
    26101 cataacgctg aagtgactgg cttcatccgc ttcgaagtgg gtgaaggcat cgagaaagtt
    26161 gagactgact ttgcagcaga agttgctgcg atgtccaagc agtcttaatt atcaaaaagg
    26221 agccgcctga gggcggcttc tttttgtgcc catcttgtaa attcagctaa cccttgtggg
    26281 gctgcgctga aaagcgacgt acaatgtcgc tagtattaat tcatttcaat cgttgacagt
    26341 ctcaggaaag aaacatggct accaatgcaa aacccgtcta taaacgcatt ctgcttaagt
    26401 tgagtggcga agctctgcag ggcactgaag gcttcggtat tgatgcaagc atactggatc
    26461 gtatggctca ggaaatcaaa gaactggttg aactgggtat tcaggttggt gtggtgattg
    26521 gtgggggtaa cctgttccgt ggcgctggtc tggcgaaagc gggtatgaac cgcgttgtgg
    26581 gcgaccacat ggggatgctg gcgaccgtaa tgaacggcct ggcaatgcgt gatgcactgc
    26641 accgcgccta tgtgaacgct cgtctgatgt ccgctattcc attgaatggc gtgtgcgaca
    26701 gctacagctg ggcagaagct atcagcctgt tgcgcaacaa ccgtgtggtg atcctctccg
    26761 ccggtacagg taacccgttc tttaccaccg actcagcagc ttgcctgcgt ggtatcgaaa
    26821 ttgaagccga tgtggtgctg aaagcaacca aagttgacgg cgtgtttact gctgatccgg
    26881 cgaaagatcc aactgcaacc atgtacgagc aactgactta cagcgaagtg ctggaaaaag
    26941 agctgaaagt catggacctg gcggccttca cgctggctcg tgaccataaa ttaccgattc
    27001 gtgttttcaa catgaacaaa ccgggtgcgc tgcgccgtgt ggtaatgggt gaaaaagaag
    27061 ggactttaat cacggaataa ttcccgtgat ggataaataa gggtaagatt ccgcgtaagt
    27121 atcgcggggg atcttacccg taatatgttt aatcagggct atacttagca cacttccact
    27181 gtgtgtgact gtctggtctg actgagacaa gttttcaagg attcgtaacg tgattagcga
    27241 tatcagaaaa gatgctgaag tacgcatgga caaatgcgta gaagcgttca aaacccaaat
    27301 cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg gatggcattg tcgtggaata
    27361 ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta acggtagaag attcccgtac
    27421 tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc gttgaaaaag cgattatggc
    27481 gtccgatctt ggcctgaacc cgaactctgc gggtagcgac atccgtgttc cgctgccgcc
    27541 gctgacggaa gaacgtcgta aagatctgac caaaatcgtt cgtggtgaag cagaacaagc
    27601 gcgtgttgca gtacgtaacg tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa
    27661 agataaagag atcagcgaag acgacgatcg ccgttctcag gacgatgtac agaaactgac
    27721 tgatgctgca atcaagaaaa ttgaagcggc gctggcagac aaagaagcag aactgatgca
    27781 gttctgattt cttgaacgac aaaaacgccg ctcagtagat ccttgcggat cggctggcgg
    27841 cgttttgctt tttattctgt ctcaactctg gatgtttcat gaagcaactc accattctgg
    27901 gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt gcgccataat cccgaacact
    27961 tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg
    28021 aattctctcc ccgctatgcc gtaatggacg atgaagcgag tgcgaaactt cttaaaacga
    28081 tgctacagca acagggtagc tgcaccgaag tcttaagtgg gcaacaagcc gcttgcgata
    28141 tggcagcgct tgaggatgtt gatcaggtga tggcagccat tgttggcgct gctgggctgt
    28201 tacctacgct tgctgcgatc cgcgcgggta aaaccatttt gctggccaat aaagaatcac
    28261 tggttacctg cggacgtctg tttatggacg ccgtaaagca gagcaaagcg caattgttac
    28321 cagtcgatag cgaacataac gccatttttc agagtttacc gcaacctatc cagcataatc
    28381 tgggatacgc tgaccttgag caaaatggcg tggtgtccat tttacttacc gggtctggtg
    28441 gccctttccg tgagacgcca ttgcgcgatt tggcaacaat gacgcccgat caagcctgcc
    28501 gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga ttcggctacc atgatgaaca
    28561 aaggtctgga atacattgaa gcgcgttggc tgtttaacgc cagcgccagc cagatggagg
    28621 tgctgattca cccgcagtca gtgattcact caatggtgcg ctatcaggac ggcagtgttc
    28681 tggcgcagct gggggaaccg gatatgcgta cgccaattgc ccacaccatg gcatggccga
    28741 atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa actaagtgcg ttgacatttg
    28801 ccgcaccgga ttatgatcgt tatccatgcc tgaaactggc gatggaggcg ttcgaacaag
    28861 gccaggcagc gactacagca ttgaatgccg caaacgaaat caccgttgct gcttttcttg
    28921 cgcaacaaat ccgctttacg gatatcgctg cgttgaattt atccgtactg gaaaaaatgg
    28981 atatgcgcga accacaatgt gtggacgatg tgttatctgt tgatgcgaac gcgcgtgaag
    29041 tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata atccggctac agagagtcgc
    29101 gctatttgtt agcgtagggc ttcagtgata tagtctgcgc catctgatcg taagtagttg
    29161 gctttataag gtcaggtatg ccgtggtttt acacggcttt tttttgtata ggcttcagta
    29221 ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc gtgatgttgt ctgctactca
    29281 accacttagc gaaaaattgc cagcgcatgg ctgccgtcat gttgcgatca ttatggacgg
    29341 caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc tttgggcata aagccggggc
    29401 aaaatccgtc cgccgggctg tctcttttgc ggccaacaac ggtattgagg cgttaacgct
    29461 gtatgccttt agtagtgaaa actggaaccg accagcgcag gaagtcagtg cgttaatgga
    29521 actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg caccgacata acgtgcgtct
    29581 gcgtattatt ggcgatacca gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc
    29641 tgaagcgcta acagccggga ataccggtct gacgctgaat attgcggcga actacggtgg
    29701 acgttgggat atagtccagg gagtcaggca actggctgaa aaggtgcagc aaggaaacct
    29761 gcaaccagat cagatagatg aagagatgct aaaccagcat gtctgtatgc atgaactggc
    29821 ccctgtagat ttagtaatta ggactggggg ggagcatcgc attagtaact ttttgctttg
    29881 gcaaattgcc tatgccgaac tttactttac agatgttctc tggcccgatt tcgatgaaca
    29941 agactttgaa ggggcgttaa atgcctttgc taatcgagag cgtcgtttcg gcggcaccga
    30001 gcccggtgat gaaacagcct gatgggggtc gcttttgctg aagtatcgcc tgatatctgc
    30061 ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc
    30121 cattgtaacg ctggtggtct gcatgctggc agcgtgggaa tggggacagc ttagcggttt
    30181 taccactcgt tcgcagcgag tatggttggc ggtgttatgc gggttattgt tggcgctgat
    30241 gctttttctg ttgccggaat atcaccgaaa tattcatcaa ccgctggttg aaatctcact
    30301 ttgggcttcg ctgggttggt ggattgtcgc gctattgctg gtgctgtttt acccaggttc
    30361 cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt tttggcgtgc taaccattgt
    30421 tcccttcttc tggggcatgc tggcgttacg ggcctggcac tatgacgaga atcattacag
    30481 tggcgcaata tggctgctct atgtcatgat cctggtatgg ggcgctgact ccggagcata
    30541 tatgtttggc aaattgtttg gtaaacataa gctggcaccg aaggtttctc cgggtaaaac
    30601 ctggcaaggc tttatcggtg gactcgctac tgcagcggta atctcatggg gttatggcat
    30661 gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt tgctctattg tcgcagcgtt
    30721 agcctcagtg ctcggcgatc tgaccgagag tatgtttaag cgtgaagcag gaattaagga
    30781 cagcggtcat ttaattccag gacacggtgg tattttagat cgtattgata gcctgacggc
    30841 tgcggtaccg gtctttgctt gcttgttgtt actggtattc aggacgcttt aacggaaggt
    30901 aatatgctga gttttctctg ggatttggct tcgttcatcg ttgcactggg tgtacttatc
    30961 accgtgcatg aatttggtca tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt
    31021 ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg ataagctcgg caccgaatat
    31081 gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg
    31141 gtcgttccgg aactccgcca ccatgccttc aataataaat ctgtcggcca acgagcggcg
    31201 attattgccg caggtccggt tgcaaacttc atttttgcta tctttgccta ctggctggtt
    31261 tttattattg gtgtgcctgg cgtacgtccg gtggttggcg aaatagcagc caattcgata
    31321 gctgcggaag cacaaattgc accaggtacg gaactaaaag ccgtagatgg tatcgaaacg
    31381 cctgattggg atgccgtgcg tttgcagttg gtcgataaaa ttggcgatga aagcaccacc
    31441 attacagtag cgccatttgg cagcgaccaa cggcgggatg taaagctcga tttacgtcac
    31501 tgggcgtttg agcctgataa agaagatccg gtatcttcgc tggggattcg tcctcgtggg
    31561 ccgcaaattg aacctgtact ggaaaatgtg cagccaaact cggcggcaag caaggcaggt
    31621 ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc
    31681 tttgtgatgc ttgtccggga taacccgggt aaatccttag cgttagaaat cgaaaggcag
    31741 ggaagtccct tgtctttgac attaatcccg gagagtaaac cgggtaatgg taaagcgatt
    31801 ggttttgtcg gtattgagcc gaaagtcatt cctttgccag atgagtataa agttgtacgc
    31861 cagtatgggc cgttcaacgc catcgtcgaa gccacggaca aaacgtggca gctgatgaag
    31921 ctgacggtca gtatgctggg aaaattgatc accggtgatg tgaaactgaa caacctcagt
    31981 gggccgatct ctatcgccaa gggggctggg atgacagcgg aactcggggt agtttattac
    32041 ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa ttaacctgtt tccgttgccc
    32101 gtacttgacg gggggcatct gctgttcctt gcgatcgaaa agatcaaggg cggaccggta
    32161 tccgagcggg ttcaagactt ttgttatcgc attggctcga ttctgctggt gctgttaatg
    32221 gggcttgcac ttttcaatga tttctctcgg ttatgagagt tagttaggaa gaacgcataa
    32281 taacgatggc gatgaaaaag ttgctcatag cgtcgctgct gtttagcagc gccaccgtat
    32341 acggtgctga agggttcgta gtgaaagata ttcatttcga aggccttcag cgtgtcgccg
    32401 ttggtgcggc cctcctcagt atgccggtgc gcacaggcga cacggttaat gatgaagata
    32461 tcagtaatac cattcgcgct ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc
    32521 gtgatggtga tacccttctg gttcaggtaa aagaacgtcc gaccattgcc agcattactt
    32581 tctccggtaa caaatcggtg aaagatgaca tgctgaagca aaacctcgag gcttctggtg
    32641 tgcgtgtggg cgaatccctc gatcgcacca ccattgccga tatcgagaaa ggtctggaag
    32701 acttctacta cagcgtcggt aaatatagcg ccagcgtaaa agctgtcgtg accccgctgc
    32761 cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg tgtgtcagct gaaatccagc
    32821 aaattaacat tgttggtaac catgctttca ccaccgacga actgatctct catttccaac
    32881 tgcgtgacga agtgccgtgg tggaacgtgg taggcgatcg taaataccag aaacagaaac
    32941 tggcgggcga ccttgaaacc ctgcgcagct actatctgga tcgcggttat gcccgtttca
    33001 acatcgactc tacccaggtc agtctgacgc cagataaaaa aggtatttac gtcacggtga
    33061 acatcaccga aggcgatcag tacaagcttt ctggcgttga agtgagcggc aaccttgccg
    33121 ggcactccgc tgaaattgag cagctgacta agatcgagcc gggtgagctg tataacggca
    33181 ccaaagtgac caagatggaa gatgacatca aaaagcttct cggtcgctat ggttatgcct
    33241 atccgcgcgt acagtcgatg cctgaaatta acgatgccga caaaaccgtt aaattacgcg
    33301 tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat ccgttttgaa ggtaacgata
    33361 cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat ggaaggtgca tggctgggaa
    33421 gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct gggcttcttt gaaactgtcg
    33481 ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt agatgtcgtc tacaaggtaa
    33541 aagagcgcaa caccggtagc ttcaactttg gtattggtta cggtactgaa agtggcgtga
    33601 gcttccaggc tggtgtgcag caggataact ggttaggtac aggttatgct gttggtatca
    33661 acgggaccaa aaacgattac cagacctatg ctgaactgtc ggtaaccaac ccgtacttca
    33721 ctgtagatgg tgtaagcctc ggtggtcgtc tcttctataa tgacttccag gcagatgacg
    33781 ccgacctgtc cgactatacc aacaagagtt atggtacaga cgtgacgttg ggcttcccga
    33841 tcaacgaata taactcgctg cgtgcaggtc tgggttatgt acataactcc ctgtccaaca
    33901 tgcagcctca ggttgcgatg tggcgttatc tgtactctat gggtgaacat ccgagcacct
    33961 ctgatcagga taatagcttc aaaacggacg acttcacgtt caactatggt tggacctata
    34021 acaagcttga ccgtggttac ttcccgacag atggttcacg tgtcaacctg accggtaaag
    34081 tgaccattcc tggatcggat aacgaatact acaaagtgac gttagacacg gcgacttatg
    34141 tgccgatcga tgacgatcac aaatgggttg ttctggggcg tacccgctgg ggttatggtg
    34201 atggtttagg cggcaaagag atgccgttct acgagaactt ctatgccggt ggttccagca
    34261 ccgtgcgtgg cttccagtcc aatacaattg gtccgaaagc agtttacttc ccgcatcagg
    34321 ccagtaatta tgatccggac tatgattacg aatgtgcgac tcaggacggc gcgaaagacc
    34381 tgtgtaaatc ggatgatgct gtaggcggta acgccatggc ggttgccagc ctcgagttca
    34441 tcaccccgac gccgtttatt agcgataagt atgctaactc ggttcgtact tccttcttct
    34501 gggatatggg taccgtttgg gatacaaact gggattccag ccaatattct ggatatccgg
    34561 actatagtga tccaagcaat atccgtatgt ctgcgggtat cgcattacaa tggatgtccc
    34621 cattggggcc gttggtgttc tcctacgccc agccgttcaa aaagtacgat ggagacaagg
    34681 cagaacagtt ccagtttaac atcggtaaaa cctggtaagt gttctccaca aaggaatgta
    34741 gtggtagtgt agcgatgact ttaggcgatc aatataagat cgccgggcca cgcaaagaac
    34801 tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg tgaaaaagtg gttattagct
    34861 gcaggtctcg gtttagcact ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc
    34921 aacatgggca gcctgttcca gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa
    34981 aatgagttca aaggccgtgc cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa
    35041 atgaaaaagc tgcagtccat gaaagcgggc agcgatcgca ctaagctgga aaaagacgtg
    35101 atggctcagc gccagacttt tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt
    35161 cgttccaacg aagaacgcgg caaactggtt actcgtatcc agactgctgt gaaatccgtt
    35221 gccaacagcc aggatatcga tctggttgtt gatgcaaacg ccgttgctta caacagcagc
    35281 gatgtaaaag acatcactgc cgacgtactg aaacaggtta aataagtaat gccttcaatt
    35341 cgactggctg atttagcgca gcagttggat gcagaactac acggtgatgg cgatatcgtc
    35401 atcaccggcg ttgcgtccat gcaatctgca caaacaggtc acattacgtt catggttaac
    35461 ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg cggttgtcat gacccaggac
    35521 gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga atccctacct gacttacgcg
    35581 cgcatggcgc aaattttaga taccacgccg cagcccgcgc agaacattgc acccagtgcg
    35641 gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt
    35701 gagtccggcg ttgaactggg cgataacgtg attatcggtg ccggttgctt cgtaggtaaa
    35761 aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg taaccattta ccatgagatc
    35821 cagatcggtc agaattgcct gatccagtcc ggaacagtgg taggcgcaga cggctttggt
    35881 tatgccaacg atcgtggtaa ctgggtgaag atcccacaga ttggtcgcgt aattattggc
    35941 gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg gcgcgctgga tgacactatt
    36001 attggcaatg gcgtgatcat tgataaccag tgccagattg cacataacgt cgtgattggc
    36061 gacaatacgg cggttgccgg tggcgtcatt atggcgggca gcctgaaaat tggtcgttac
    36121 tgcatgatcg gcggagccag cgtaatcaac gggcatatgg aaatatgcga caaagtgacg
    36181 gttacgggca tgggtatggt gatgcgtccc atcactgaac caggcgtcta ttcctcaggc
    36241 attccgctgc aacccaacaa agtctggcgc aaaaccgctg cactggtgat gaacattgat
    36301 gacatgagca agcgtctgaa atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc
    36361 atcttttgtt cgccaaactt tacggcctgt ctcattctta cgattgcggc aggccgtgtt
    36421 attattgtcg tttcttatat tttgacagga agagtatctt gactactaac actcatactc
    36481 tgcagattga agagatttta gaacttctgc cgcaccgttt ccctttctta ctggtggatc
    36541 gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg
    36601 agccattctt ccagggccat ttccctggaa aaccgatttt cccgggtgtg ctgattctgg
    36661 aagcaatggc acaggcaaca ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg
    36721 gtgagctgta ctatttcgct ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg
    36781 gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta
    36841 aaggggttgc tctggtcgac ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta
    36901 gccgggaggc ctgatacgtg attgataaat ccgcctttgt gcatccaacc gccattgtgg
    36961 aagagggcgc gtcgattggc gcgaacgcac acattggtcc tttttgtatc gttggacccc
    37021 atgtcgaaat tggtgagggt accgtactga aatctcacgt tgtcgtgaat ggccatacta
    37081 aaattggccg cgataatgag atttatcagt tcgcctccat cggtgaagtt aaccaggatc
    37141 tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga tcgtaaccgc attcgcgaaa
    37201 gcgtcaccat tcatcgtggc acagtccagg gcggtggatt gacgaaggtg ggcagcgaca
    37261 acttactgat gatcaacgcg cacattgcgc acgattgtac ggtaggtaac cgctgtattc
    37321 tcgccaacaa cgcaacgctg gcgggtcacg tatcggtaga cgacttcgcg atcatcggcg
    37381 gcatgaccgc agtccatcag ttctgcatca ttggtgcgca tgtgatggtt ggcggttgct
    37441 ccggtgtggc gcaggacgtc cctccttatg tcattgcgca gggtaaccac gcaacgccgt
    37501 tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag ccgcgaggcg attaccgcta
    37561 tccgcaatgc gtataagctg atttatcgca gcggtaaaac gctcgatgaa gtgaaaccgg
    37621 aaattgctga actggcggaa acgtatccgg aagtgaaagc ttttaccgat ttctttgcac
    37681 gctcaacgcg cggtctgatt cgttaatgac taaacagcgt ccattaacga ttgccctggt
    37741 cgccggagaa acctccggcg atatcctggg ggccggttta atccgcgctc tgaaagaacg
    37801 tgtgcccaac gcccgctttg ttggcgttgc cgggccacga atgcaggctg aaggctgcga
    37861 agcctggtac gaaatggaag aactggcggt gatgggcatt gttgaagtgc tcggtcgtct
    37921 gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt tttggtgaac taaagccaga
    37981 tgtttttgtt ggcattgatg cgcctgactt caatattacc ctcgaaggta accttaaaaa
    38041 gcagggtatc aaaaccattc attacgtcag tccatccgtc tgggcgtggc gacagaaacg
    38101 cgttttcaaa ataggcagag ccaccgatct ggtgctcgca tttctgcctt tcgaaaaagc
    38161 gttttatgac aaatacaatg taccgtgccg ctttatcggt cataccatgg ctgatgccat
    38221 gccgttagat ccagataaaa atgccgcccg tgatgtgctg gggatccctc acgatgccca
    38281 ctgcctggcg ttgctaccag ggagccgtgg tgcagaagtt gaaatgctta gtgccgattt
    38341 cctgaaaacg gcccagcttt tgcgccagac atatccggat ctcgaaatcg tggtgccact
    38401 ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa gctgaagtcg cgccagacct
    38461 ttcagttcat ttgctggatg ggatgggccg tgaggcgatg gtcgccagcg atgcggcgct
    38521 actggcgtcg ggtacggcag ccctggagtg tatgctggcg aaatgcccga tggtggtggg
    38581 atatcgcatg aagcctttta ccttctggtt ggcgaagcgg ctggtgaaaa ctgattatgt
    38641 ctcgctgcca aatctgctgg cgggtagaga gttagtcaaa gaattattgc aggaagagtg
    38701 tgagccgcaa aaactggctg cggcgctgtt accgctgttg gcgaacggga aaaccagcca
    38761 cgcgatgcac gataccttcc gtgaactgca tcagcagatc cgctgcaatg ccgatgagca
    38821 ggcggcacaa gccgttctgg agttagcaca atgatcgaat ttgtttatcc gcacacgcac
    38881 ctggttgcgg gtgtggatga agtcggacgc gggccgttag ttggcgcggt cgtcaccgct
    38941 gcggtgatcc tcgacccggc gcgcccgatt gccgggctga atgattccaa aaagctgagc
    39001 gaaaaacgcc gtctggcgct ctgtgaagag atcaaagaga aagcgttgag ctggagtctg
    39061 ggccgcgcgg aaccccacga aatcgacgag ctgaacattc ttcatgcgac catgctggcg
    39121 atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat atgtgttgat tgatggtaac
    39181 cgctgcccga aattaccgat gcctgcgatg gctgtggtga aaggcgatag ccgcgtaccg
    39241 gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg acgccgaaat ggcggcgctg
    39301 gatattgttt tcccgcaata tggttttgcc caacacaaag ggtatccaac cgcttttcat
    39361 ctggaaaaac tggctgaata tggcgcgact gaacatcatc gtcgcagctt tgggcctgtc
    39421 aaacgagcac tgggacttgc gtcctgattc ttgtcgagat taagtaaacc ggaatctgaa
    39481 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    39541 cctggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    39601 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    39661 agggattaag cctatcgtcg gggcagattt taacgtccag tgcgacctgc tgggtgatga
    39721 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tgacgttgct
    39781 gatctcaaaa gcgtatcagc gtgggtacgg tgccgctggg ccgatcatcg atcgcgactg
    39841 gcttatcgaa ttaaatgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    39901 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    39961 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    40021 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa
    40081 cgacgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    40141 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    40201 gcgtagcgaa gaggagatgt gcgagctgtt tgccgacatc ccagaagctc ttgccaacac
    40261 cgttgagatc gccaaacgct gtaacgtgac tgtgcgtctt ggtgaatact tcctgccgca
    40321 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    40381 ggaagagcgt ctggcctttt tattccctga tgaggaagaa cgtcttaaac gccgcccgga
    40441 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggct tcccgggcta
    40501 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtagggcc
    40561 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    40621 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    40681 tgacttcgac gttgacttct gtatggagaa acgcgaccag gttatcgagc acgtagcgga
    40741 catgtacggt cgtgatgcgg tatcgcagat catcaccttc ggtacaatgg cggcgaaagc
    40801 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc
    40861 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    40921 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    40981 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    41041 caaaattacc gattttgcgc cgctttactg cgatgaagag ggcaaacatc cggtcaccca
    41101 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    41161 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    41221 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    41281 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    41341 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    41401 tggtccgttg cagtcaggga tggtggataa ctttattgac cgtaaacatg gtcgcgaaga
    41461 aatctcctat ccagacgtac agtggcagca tgaaagcctg aaaccggtac tggagccaac
    41521 ctacggcatt atcctgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    41581 taccctcggt ggcgcggata tgctgcgtcg agcgatgggt aagaaaaagc cggaagagat
    41641 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    41701 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    41761 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    41821 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    41881 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    41941 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg cgatcaaagg
    42001 ggtcggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    42061 cgaactgttt gatctctgcg cccgaaccga caccaaaaag ttaaaccggc gggtgctgga
    42121 aaaactgatc atgtccgggg cgtttgaccg tcttggacca caccgcgcgg cactgatgaa
    42181 ctcgctgggc gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    42241 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    42301 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    42361 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    42421 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacggctg cggggctcgt
    42481 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    42541 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    42601 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    42661 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    42721 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    42781 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    42841 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    42901 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    42961 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    43021 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    43081 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt
    43141 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccacagc gtccttatac
    43201 cctggattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgcgcgta
    43261 tgcagacgat aaagctatcg tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat
    43321 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    43381 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    43441 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    43501 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt
    43561 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    43621 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    43681 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    43741 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    43801 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    43861 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    43921 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    43981 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    44041 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    44101 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    44161 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    44221 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    44281 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    44341 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccgac
    44401 gaatatcttg ataacattac accgccgttc acgaaagcct tgtttaccta cgttaaagag
    44461 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    44521 gttggctgcc tgttttatga ttttttcggc gggaataccc tcaaggctga tgtctctatt
    44581 tcggtcaccg agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag
    44641 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    44701 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    44761 aattgtcata aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    44821 aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc cgcgccgtga atttactcgc
    44881 gacagcatcg aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg
    44941 attaccaact ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg
    45001 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc
    45061 taccagggga aaagtggtat gagcggcgag cgtgttgcgg gaaaagtgat cttcgaaacg
    45121 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cgctgatcca cattaaaggc
    45181 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    45241 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    45301 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggttg
    45361 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    45421 gaatgctggc ccgttgcgcc aggcgaacag tggcacggct ttaacgatgc ggatgccgat
    45481 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    45541 aatatgagcg aggaggggat cccggcagcg ctggtggcaa aattcctcga cgaacgtggg
    45601 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggtatcgat
    45661 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaaacgctc ttacgatctc
    45721 aacctgcgga tcaaaaatat gctacccgat ctctatgctg aagatcccga tttctaccgc
    45781 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    45841 cccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    45901 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    45961 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    46021 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    46081 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    46141 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    46201 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    46261 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    46321 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    46381 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    46441 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgatatc gatgcggcag
    46501 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    46561 aaaaacgctt cacgttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    46621 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    46681 accatcatga cactcacgct caatagacaa cttctcacct tacgccagat tctggtggcc
    46741 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    46801 aatccgggcg tcactctacg cgctatccat gtgcatcacg gtttaagtgc caatgccgat
    46861 gcctgggtta ggcattgcga gaacatctgc caacagtggc aggtgccgct ggtggtcgag
    46921 cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc aggcgcggca ggcgcgttat
    46981 caggcatttg cccgcacctt gttgcccggt gaagtgctgg tcaccgcgca acatctcgac
    47041 gatcaatgtg aaacttttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    47101 gctatggcgg aagtttcgga gtttgccgga acacggctta tccgcccgtt gcttgcccgc
    47161 acgcgggggg aactggcgca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    47221 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    47281 cagcagcgtt ggccgcattt tgccgaagca acggcccgca gcgctgcgct ttgcgctgaa
    47341 caagagagcc tgttagatga actgctggcg gatgatatag cgcactgtca aaagccgcag
    47401 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatc
    47461 cgccgctggc tggcagggaa gaatgcaccg atgccttccc gcgacgcgtt ggtgcggatc
    47521 tggcaggaag tggcgctggc gcgggaagat gcctccccct gtttacgctt gggcgcgttt
    47581 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagtgaa
    47641 accattgtgc cgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaagc
    47701 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    47761 cgtttcaaag cgccaggatt gctgcatatt gtcggtcgca acggcggacg taagctaaag
    47821 aaaatctggc aagagctggg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    47881 tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    47941 ggtgagaagg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    48001 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat ttgcttacca ccaccgtacc
    48061 gatttccggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    48121 ggcctcgacg actagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    48181 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    48241 aagattatcg taatcatcac aattgattgg ttgatacgta tcattcattg acataatcgc
    48301 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    48361 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    48421 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    48481 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    48541 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    48601 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    48661 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgtaaccagt ttctcgatgc
    48721 ctctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    48781 ggcgtggttg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    48841 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    48901 gctgccggat gagcggcgga ttaagaaagc ctgcacccag gccgcaaaag tggcgctgtt
    48961 tacctataat agccgggcgg cgcaaatctg gtggcagcaa aatcagagca aatttgtgca
    49021 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    49081 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggcgtgatct ggttatcgga
    49141 tgataagaat aatctggaag tgaacttaac cgcctggcaa caaccttcat gattgtgatt
    49201 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    49261 gcgggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    49321 gcgtccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    49381 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    49441 gaagcggcgc tggcccggct ggtggcagtg attaaagatt taacaacaga acaaaaagcc
    49501 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    49561 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    49621 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    49681 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactaaa
    49741 accgatgccg caaagttggc gcggcgtgct gccgtgtgcc gattgcgaag ggatcgaaac
    49801 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    49861 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    49921 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    49981 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    50041 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    50101 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    50161 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    50221 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    50281 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaacccgt
    50341 cttgagacag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    50401 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    50461 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    50521 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaat
    50581 gcagagacat cgccagtacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    50641 gccagcgtaa gcggatgccc ggctttggca aatgcatcag tgacaaactc cgaacaaaac
    50701 cacgactttt tgtctccttc gcccacactg cttaactgcg ctttcgccag gccgctgacg
    50761 cactgttggc gaaaatctgc ggaaaaagga ttcagtgagc acatctggcg ggtcaccata
    50821 aatggaatga actcgacaat gccgcgatag ttatacccgc tatctttgat tttgttggcg
    50881 aacgcggtga tttctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    50941 ttatcgctat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    51001 tctgcaacgt tattttcacc cagatagatt gcaacgtgac tcacagagga agtgctgaag
    51061 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    51121 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    51181 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    51241 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    51301 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    51361 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    51421 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    51481 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    51541 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    51601 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    51661 ctggaacggc gcgatcgcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcgat
    51721 cgccgcagct actacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttgatt
    51781 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    51841 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctgac catccgggct
    51901 tggatcgccc gccaccacgt tgcggatatc agcaatttcc ggggtagcga catcgcgatc
    51961 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    52021 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    52081 cggaccggct ttaaccacgg cacgaatttc ttcttcagtc gcaaaagtca gcgggctggc
    52141 aacctgcggc agtttttctg ctttaacttc gttcagctcg tggtcaccgc gcaccagcag
    52201 cgcaaccaac gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    52261 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    52321 aaccagcgtc atttcctggg tagccgcagc gcgcggttct ttcggcgcga tagcttctgc
    52381 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    52441 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    52501 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    52561 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    52621 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    52681 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    52741 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    52801 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    52861 ctgaaccacc ggcatcgaca cctcgattgc accggcgttg ttcatctctt cacgcacgat
    52921 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    52981 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    53041 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    53101 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    53161 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    53221 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    53281 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    53341 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    53401 tctgcggtaa aactcaccgc catttctgcc gctggcgcac tttgcgcata gctggcactg
    53461 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    53521 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    53581 ttcagctcta ccagcgacat accaattggg ttagggcgga aagtagagcg cgtagcgaaa
    53641 acacccattc tggcattacc gccaagacga ggcggacgca cagtcggacg ccagccgcct
    53701 tccatcgttt gatgaaagac gaaaagtatc cataaatggc tgaacgcttc caggccgcga
    53761 acggcgtcgg cctggttgta gggagccatg agatgcagtt cgccgttggc gctttttacc
    53821 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    53881 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    53941 cctgacgata gcagcctggc gtaccgctgg taacttcgca gctatgcagt aataccgcat
    54001 tggctttcat tttcgaggcg ttgatttgca tccgcttacg tgcggttgga atgctcggcg
    54061 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    54121 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    54181 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    54241 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    54301 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    54361 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    54421 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    54481 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    54541 ttacgatcag gtttacgtac ggggagtctt tatcttcgac aaagataccg tctttcgctg
    54601 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    54661 cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac aattttcaga ttttttgggt
    54721 tctcaacaac atcaagaacg gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    54781 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    54841 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    54901 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    54961 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    55021 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    55081 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    55141 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    55201 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    55261 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    55321 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttat
    55381 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    55441 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    55501 gcagcgcttc cggtaacagc accttacgga cgatctgcat cggcgtggca cccattgcgc
    55561 gggaagcttc aattaacccg gttgggatct ctagcagagc gttctcgacc atacgggcaa
    55621 taaacggcgc tgcgccaacg gttaacggaa caatcgccgc ctgtaaacca atcgatgtac
    55681 cgacaataac tcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    55741 ggaaaatgtt cacaattgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    55801 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaag ccaaaaaaac
    55861 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    55921 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    55981 gcttgcgtat cttgttgtgt gccgtgcatt tcggtcagca tgatgccgaa cttaacgcca
    56041 ccggcgtaat ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    56101 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    56161 cagtcagtaa atggctccgc ttgcagacgt tcctggtaat cttccgggat atccagatgc
    56221 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    56281 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatgcgc
    56341 ttcacaacgt ccatttcgtg agtgatcaac agaatcgtca aacccagacg gcggttgatg
    56401 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    56461 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    56521 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    56581 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    56641 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    56701 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcag ttcctggcca
    56761 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    56821 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    56881 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    56941 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtggca tctcgaatgc
    57001 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    57061 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcacatgc
    57121 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    57181 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    57241 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    57301 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    57361 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    57421 catcatccgc agggtagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    57481 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    57541 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    57601 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    57661 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc ataatgatta
    57721 aaagatgagc gattgaaata aaaatgcatt tttccacttg tcttcctgag acgactccct
    57781 ataatgcgcc tccatcgaca cggcggatgt gaatcacttc acacaaacag ccggttcggt
    57841 tgaagagaaa aatcctgaaa ttcagggttg actctgaaag aggaaagcgt aatatacgcc
    57901 acctcgcgac agtacgctaa agcg
//
LOCUS       DAPHJE010000008        57496 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPHJE010000008 DAPHJE010000000
VERSION     DAPHJE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.008"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..117
                     /locus_tag="RH222_000567"
     tRNA            42..117
                     /locus_tag="RH222_000567"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Lys,seq:ttt)
     gene            238..579
                     /locus_tag="RH222_000568"
     CDS             238..579
                     /locus_tag="RH222_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDV3795633.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(570..1496)
                     /locus_tag="RH222_000569"
     CDS             complement(570..1496)
                     /locus_tag="RH222_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3795634.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1586..2584
                     /locus_tag="RH222_000570"
     CDS             1586..2584
                     /locus_tag="RH222_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV3795635.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2581..2799)
                     /locus_tag="RH222_000571"
     CDS             complement(2581..2799)
                     /locus_tag="RH222_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV3795636.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2801..4816)
                     /gene="ligA"
                     /locus_tag="RH222_000572"
     CDS             complement(2801..4816)
                     /gene="ligA"
                     /locus_tag="RH222_000572"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV3795637.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4887..5873)
                     /gene="zipA"
                     /locus_tag="RH222_000573"
     CDS             complement(4887..5873)
                     /gene="zipA"
                     /locus_tag="RH222_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV3795638.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6103..6864
                     /gene="cysZ"
                     /locus_tag="RH222_000574"
     CDS             6103..6864
                     /gene="cysZ"
                     /locus_tag="RH222_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV3795639.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7049..8020
                     /gene="cysK"
                     /locus_tag="RH222_000575"
     CDS             7049..8020
                     /gene="cysK"
                     /locus_tag="RH222_000575"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV3795640.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8404..8661
                     /gene="ptsH"
                     /locus_tag="RH222_000576"
     CDS             8404..8661
                     /gene="ptsH"
                     /locus_tag="RH222_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV3795641.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8706..10433
                     /gene="ptsI"
                     /locus_tag="RH222_000577"
     CDS             8706..10433
                     /gene="ptsI"
                     /locus_tag="RH222_000577"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV3795642.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10474..10983
                     /gene="crr"
                     /locus_tag="RH222_000578"
     CDS             10474..10983
                     /gene="crr"
                     /locus_tag="RH222_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV3795643.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11025..11876)
                     /gene="pdxK"
                     /locus_tag="RH222_000579"
                     /gene_synonym="thiJ"
     CDS             complement(11025..11876)
                     /gene="pdxK"
                     /locus_tag="RH222_000579"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV3795644.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            11981..12349
                     /locus_tag="RH222_000580"
     CDS             11981..12349
                     /locus_tag="RH222_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV3795645.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12352..13263)
                     /gene="cysM"
                     /locus_tag="RH222_000581"
     CDS             complement(12352..13263)
                     /gene="cysM"
                     /locus_tag="RH222_000581"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV3795646.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13397..14494)
                     /gene="cysA"
                     /locus_tag="RH222_000582"
     CDS             complement(13397..14494)
                     /gene="cysA"
                     /locus_tag="RH222_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV3795647.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14484..15359)
                     /gene="cysW"
                     /locus_tag="RH222_000583"
     CDS             complement(14484..15359)
                     /gene="cysW"
                     /locus_tag="RH222_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV3795648.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLSNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15359..16192)
                     /gene="cysT"
                     /locus_tag="RH222_000584"
     CDS             complement(15359..16192)
                     /gene="cysT"
                     /locus_tag="RH222_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV3795649.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16192..17208)
                     /gene="cysP"
                     /locus_tag="RH222_000585"
     CDS             complement(16192..17208)
                     /gene="cysP"
                     /locus_tag="RH222_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV3795650.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(17379..18170)
                     /gene="ucpA"
                     /locus_tag="RH222_000586"
     CDS             complement(17379..18170)
                     /gene="ucpA"
                     /locus_tag="RH222_000586"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV3795651.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(18299..19156)
                     /gene="murR"
                     /locus_tag="RH222_000587"
     CDS             complement(18299..19156)
                     /gene="murR"
                     /locus_tag="RH222_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV3795652.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            19320..20216
                     /gene="murQ"
                     /locus_tag="RH222_000588"
     CDS             19320..20216
                     /gene="murQ"
                     /locus_tag="RH222_000588"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV3795653.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            20220..21644
                     /gene="murP"
                     /locus_tag="RH222_000589"
     CDS             20220..21644
                     /gene="murP"
                     /locus_tag="RH222_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV3795654.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            21649..22953
                     /gene="pbp4b"
                     /locus_tag="RH222_000590"
     CDS             21649..22953
                     /gene="pbp4b"
                     /locus_tag="RH222_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV3795655.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(23011..23910)
                     /gene="yfeX"
                     /locus_tag="RH222_000591"
     CDS             complement(23011..23910)
                     /gene="yfeX"
                     /locus_tag="RH222_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV3795656.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24006..24581)
                     /locus_tag="RH222_000592"
     CDS             complement(24006..24581)
                     /locus_tag="RH222_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV3795657.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24642..25091)
                     /locus_tag="RH222_000593"
     CDS             complement(24642..25091)
                     /locus_tag="RH222_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV3795658.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(25078..25503)
                     /locus_tag="RH222_000594"
     CDS             complement(25078..25503)
                     /locus_tag="RH222_000594"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV3795659.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25717..26586
                     /gene="amiA"
                     /locus_tag="RH222_000595"
     CDS             25717..26586
                     /gene="amiA"
                     /locus_tag="RH222_000595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV3795660.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26590..27489
                     /gene="hemF"
                     /locus_tag="RH222_000596"
     CDS             26590..27489
                     /gene="hemF"
                     /locus_tag="RH222_000596"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV3795661.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27495..28547)
                     /gene="eutR"
                     /locus_tag="RH222_000597"
     CDS             complement(27495..28547)
                     /gene="eutR"
                     /locus_tag="RH222_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV3795662.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMVMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(28593..29093)
                     /gene="eutK"
                     /locus_tag="RH222_000598"
     CDS             complement(28593..29093)
                     /gene="eutK"
                     /locus_tag="RH222_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV3795663.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29106..29765)
                     /gene="eutL"
                     /locus_tag="RH222_000599"
     CDS             complement(29106..29765)
                     /gene="eutL"
                     /locus_tag="RH222_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV3795664.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(29775..30662)
                     /gene="eutC"
                     /locus_tag="RH222_000600"
     CDS             complement(29775..30662)
                     /gene="eutC"
                     /locus_tag="RH222_000600"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV3795665.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(30683..32044)
                     /gene="eutB"
                     /locus_tag="RH222_000601"
     CDS             complement(30683..32044)
                     /gene="eutB"
                     /locus_tag="RH222_000601"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV3795666.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(32056..33459)
                     /gene="eutA"
                     /locus_tag="RH222_000602"
     CDS             complement(32056..33459)
                     /gene="eutA"
                     /locus_tag="RH222_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV3795667.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(33456..34682)
                     /gene="eutH"
                     /locus_tag="RH222_000603"
     CDS             complement(33456..34682)
                     /gene="eutH"
                     /locus_tag="RH222_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV3795668.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(34799..35986)
                     /gene="eutG"
                     /locus_tag="RH222_000604"
     CDS             complement(34799..35986)
                     /gene="eutG"
                     /locus_tag="RH222_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV3795669.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(35976..36812)
                     /gene="eutJ"
                     /locus_tag="RH222_000605"
     CDS             complement(35976..36812)
                     /gene="eutJ"
                     /locus_tag="RH222_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV3795670.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(36823..38226)
                     /locus_tag="RH222_000606"
     CDS             complement(36823..38226)
                     /locus_tag="RH222_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV3795671.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(38238..38525)
                     /gene="eutN"
                     /locus_tag="RH222_000607"
     CDS             complement(38238..38525)
                     /gene="eutN"
                     /locus_tag="RH222_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV3795672.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(38632..38925)
                     /gene="eutM"
                     /locus_tag="RH222_000608"
     CDS             complement(38632..38925)
                     /gene="eutM"
                     /locus_tag="RH222_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV3795673.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(38964..39980)
                     /gene="pta"
                     /locus_tag="RH222_000609"
     CDS             complement(38964..39980)
                     /gene="pta"
                     /locus_tag="RH222_000609"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV3795674.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            complement(39977..40780)
                     /gene="eutT"
                     /locus_tag="RH222_000610"
     CDS             complement(39977..40780)
                     /gene="eutT"
                     /locus_tag="RH222_000610"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV3795675.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(40777..41478)
                     /gene="eutQ"
                     /locus_tag="RH222_000611"
     CDS             complement(40777..41478)
                     /gene="eutQ"
                     /locus_tag="RH222_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV3795676.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(41453..41932)
                     /gene="eutP"
                     /locus_tag="RH222_000612"
     CDS             complement(41453..41932)
                     /gene="eutP"
                     /locus_tag="RH222_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV3795677.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(41945..42280)
                     /gene="eutS"
                     /locus_tag="RH222_000613"
     CDS             complement(41945..42280)
                     /gene="eutS"
                     /locus_tag="RH222_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV3795678.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(42573..44852)
                     /gene="maeB"
                     /locus_tag="RH222_000614"
     CDS             complement(42573..44852)
                     /gene="maeB"
                     /locus_tag="RH222_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV3795679.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            45141..46091
                     /gene="tal"
                     /locus_tag="RH222_000615"
     CDS             45141..46091
                     /gene="tal"
                     /locus_tag="RH222_000615"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV3795680.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            46111..48114
                     /gene="tkt"
                     /locus_tag="RH222_000616"
     CDS             46111..48114
                     /gene="tkt"
                     /locus_tag="RH222_000616"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV3795681.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(48210..49253)
                     /locus_tag="RH222_000617"
     CDS             complement(48210..49253)
                     /locus_tag="RH222_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV3795682.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNCCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(49379..49954)
                     /gene="nudK"
                     /locus_tag="RH222_000618"
     CDS             complement(49379..49954)
                     /gene="nudK"
                     /locus_tag="RH222_000618"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV3795683.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(50022..52001)
                     /gene="aegA"
                     /locus_tag="RH222_000619"
     CDS             complement(50022..52001)
                     /gene="aegA"
                     /locus_tag="RH222_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV3795684.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            52207..53907
                     /gene="narQ"
                     /locus_tag="RH222_000620"
     CDS             52207..53907
                     /gene="narQ"
                     /locus_tag="RH222_000620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV3795685.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            54071..57184
                     /gene="acrD"
                     /locus_tag="RH222_000621"
     CDS             54071..57184
                     /gene="acrD"
                     /locus_tag="RH222_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV3795686.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(57283..57342)
                     /gene="ypfM"
                     /locus_tag="RH222_000622"
     CDS             complement(57283..57342)
                     /gene="ypfM"
                     /locus_tag="RH222_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV3795687.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        13310 a        15915 c        14795 g        13476 t
ORIGIN      
        1 gagggggtcg gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg
       61 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg
      121 taaaaaaagc gccctaaagg cgcttttttg ctatctgcaa tttgtaagat tgatttttct
      181 tctcaagttg cttgttcttc taccgatatt acctcgtaca gtgacgagga ctgaacaatg
      241 gccactatct cttcaacttc aattccttcc atacaaacac agagtagtaa cagggcatca
      301 caaggtagcg atgttgcttc acaaatcgcg cggataagtc agcaaatcat caaactcact
      361 caacaaatta aagagattgt agacactagc ggcagtgctg aagacaagca aaaacaggcc
      421 gaattgattc aacagcaaat caccctactg gaaacacaac tggcgcagtt gcagaaacag
      481 caggcagaaa aggcgcagga gaaagagcag tgtttgtctc tgaatgtatc ccttctaaat
      541 cctgttgaaa aaacgactca tatcgatatc tatatctgat acaacggatc cccttcccgc
      601 ccagccatta gcgcattgcc ctggtctcgt accacgtccc ataacgcttc agcggcggtg
      661 gaaagtgaac ggttcttcct gcgcaccagc atcaactggc gttcaaccac cggcgtaatg
      721 cgcttaacca ccagtgggct accttcaggc aatggtagcg ccagtgcggg aagaatactg
      781 atgccgatgc ctgcggctac catcgggaac agcgtcgccg gatgtccaat ctcctgcaca
      841 atattcgcct gaataccatt gcgcgccagc gctgcgtcaa tcagcggtcg gctgcctgac
      901 gcgtaatcct gcaacaccaa tttcgctccc tgtaatgcct gccagggtac ataatcttcg
      961 acggccaaag cgctatcgcg atggcaaagc agaaagaaag gctcggaaag aatcgcttca
     1021 cattgcagat cgcccactgg gcctggatcg atgacgatgc caaagtcgac atctccctgg
     1081 cgaatacttt ccatcaccca ctgctgcgga cgatcgtgca ggacaaactg aatatctgga
     1141 tagcggcgat ggctttcagc aatgcattgc ggaataagat gcgcggaaat ggtctggctg
     1201 gcagcgaccc gaacttttcc gctcagttgt tgccccatac gcccggtatc gcgcaacgtg
     1261 ctgttcagtt catccagcag tcgctcaaga cgcaaagcca gctgctgccc tgcatctgta
     1321 agcaccactt cacgcgtggt tctgtccagc aggcgaacac cagtatgatt ttccagttcc
     1381 ttcacactgt gactcactgc cgactggctc aggccgatac gctctcctgc acgactaaaa
     1441 cttttctcct gcgctactgt gacgaaaacc tttaattgtt ttaaagaata attcatctgt
     1501 tttcttcatg aatggatgca ataaatcaat tttatttctc aaactggaag aagcacaata
     1561 gaaccatcga tcatctggag tctttatgaa actttttcgt atcctcgatc ctttcacctt
     1621 aaccctgatc acggtggtgt tgctggcctc tttctttccg gccagaggcg atttcgtccc
     1681 cttctttgaa aatctgacca ccgcagcgat tgccctgctg ttctttatgc acggcgcgaa
     1741 gttgtcgcgt gaggcgatta ttgctggcgg tggtcactgg cgactgcatt tgtgggtaat
     1801 ttgcagcacc ttcgtgctgt ttccgattct gggtgtactg tttgcctggt ggaaaccggt
     1861 aaatgtcgac ccgatgctct actccggttt tctctacttg tgcattctcc cggctaccgt
     1921 gcagtctgca atcgccttca cgtcaatggc gggcggtaac gtcgcggcgg cggtttgttc
     1981 tgcgtcagca tccagcctgc tggggatttt cctttcacca ttgctggttg gtctggtgat
     2041 gaatgttcac ggtgcagggg gcagccttga gcaggtcggt aaaattatgc tgcaactgct
     2101 gctgccgttt gtgttggggc atctttcccg gccgtggatt ggtgactggg tgtcgcgcaa
     2161 taaaaaatgg attgcgaaaa ctgaccagac gtccattctg ttggtggttt atacagcgtt
     2221 cagcgaagcc gtcgttaatg gtatctggca taaagttggc tggggatcat tgctgtttat
     2281 cgtggtggtc agctgcgttc ttctggctat cgtgattgta gttaacgtct ttatggcacg
     2341 ccgactgggc ttcaataagg cagatgaaat tactatcgtc ttttgtggtt cgaaaaagag
     2401 tctggcaaat ggcatcccga tggcaaacat tctgttcccc acatcggtga tcggtatgat
     2461 ggtgctgccc ctgatgattt tccatcagat ccaattgatg gtctgtgcgg tgctggcgcg
     2521 tcgatacaaa cgccagaccg aacagttaca ggcacagcag gaaagcagcg ccgataaagc
     2581 ttaaagcgga cgcttcaggg gctggaccag ttgcgtcagc ccctcggttt taatcagcag
     2641 cgtgatttgc attagctcac cgagcttccc tgccgggaat tcatctttgc gggcaaacca
     2701 cagcagatac tcctccggca ggtcgattaa gcgacgccct ttatatttgc caaacggcat
     2761 tatcgtattg gctatttcaa tcagctgctc tttttccatc tcagctaccc agcaaacgca
     2821 gcatttccgc ttcgtcgatg acttcaatgc ccagttcctg cgctttcgcc agtttagaac
     2881 ctgccgcttc tccggcaatc accagatcgg ttttcttcga cacgctgccc gcgactttcg
     2941 cgcccagttc aaccagtcga gctttagcgt catcacgcga catctggctt aagctgcccg
     3001 taagcaccac ggttttacca gcaaacgggc tgtcaatctc ttccgcgttg ataacgatcg
     3061 gcgcaggcca gtgaacacct tccgccaaca gctcgctgat gacattgcgg ttgctttctt
     3121 cggcaaagaa gttgtgaacg tgggatgcaa cgacaatgcc aacatcaggc accttttgca
     3181 gctcttcaat cgaagcggct tccagcgctt ccagcgtgcc gaaatatgcc gccagacctg
     3241 ctgcggtggc ctcgccgact tcacggatgc caagtgcata gaggaagcga gcaaaggtgg
     3301 tttctttcgc tttttccagc gcgttaacca cgttttgtgc cgattttggc cccatacgct
     3361 ccagtccggt cagtttgcct gcggtgagtt tgaacagatc tgccggagta tggacatatt
     3421 ctttttcaac cagctgatcg atgattttgt cgcccattcc gtcaacatcc atcgcacggc
     3481 gggaaacaaa gtgtttcagc gactctttac gctgcgctcc gcaaatcagg ccaccggtac
     3541 agcgggcaac cgcttcacct tccacacgct caacgtcaga accacatacc ggacaatgcg
     3601 tcgggaatac aacctcacgg gtatcttccg ggcgttcaga aagcacgacg ttaaccacct
     3661 gcgggatcac gtcgccagcg cggcgaatca ccactttatc gccaatgcgt aaaccaagac
     3721 gttcgatttc atccgcattg tgtaaggttg cgttactcac cagcacgcca gcaacatgga
     3781 caggttccag acgcgcaaca ggcgtaatcg cgccagtacg cccaacctga aactcgacgt
     3841 cacgcacaaa ggtcatctgc tcctgcgccg ggaatttaaa cgctaccgcc cagcgcgggg
     3901 cacgcgcgac aaagccaagc tgctcctgct gttccagtga gttgacctta atcaccacgc
     3961 cgtcgatatc aaagcccagc gtcgggcggt cttcttccac tttgtgatag aacgccagca
     4021 cttcttccgc cgattcacaa agcgttaccc gatcgctgac cggcaacccc cactttttaa
     4081 attgcagtaa acggccaaga tgagtatccg gcagctcgcc accttccaga acaccaacgc
     4141 catagcagaa aaaagtgagc ggtcgcttcg ctgtaatacg cggatcaagc tgacgcagtg
     4201 aaccagctgc cgcattacgt gggttagcaa aaactttccc gcccgtgcgt cgcgcatctt
     4261 cgttaatctt ttcgaacccc gcctgcggca ggaacacttc accacgcact tccagacgcg
     4321 ccgggatatt ctctccgtgc agcttcagcg gaatagcgcg aatagtacgc acattagacg
     4381 tgatatcttc cccggtggtg ccatcgccac gggtcgcggc actgactaaa acgccatttt
     4441 catacagaat actgacggca aggccatcca gcttcagctc acagcaccag gtgactttct
     4501 cgttgttttt cagacggtcc tgcacacgtt tgttgaaagc aagaaagctt tcttcatcaa
     4561 aaacgttatc cagtgacagc attggtactt catggcgtat ctggctgaaa gccgccagcg
     4621 gcgcagcgcc tacacgttgg gtaggcgaat caggcgtaat cagttctgga tgtttggttt
     4681 ccagctcgcg cagttcgcgc atcagcctgt cgtattcagc gtcgggaatt tccggcgcat
     4741 ccatcacatg ataaagatat tcatgatggc gaagcgtcgt tcgcagttct gtcagttgtt
     4801 gttcgattga ttccatatcg caccatcaat gctaaaaacc cccgacaagc gggggttcga
     4861 agaggagtta atttgcctta agtgtatcag gcgttggcgt ctttgacttc gcggatgatg
     4921 tcctggtact cgcgcaattt ctgcggagtc atcatacggc gctgatcgtc aagcacgaca
     4981 ccgcccactt catcggcaat atgctgcgca gattgcagca tcagcttgaa gttctgcagc
     5041 tcgtcaccgt aagacggtac ctgcataaag atggtgacac ccggagtagt gaaatccttc
     5101 atttcaggat caaaggttcc cggtttcacc atattcgcca ggctgaataa cgccgggccg
     5161 ctgccatccg ggctaagatg acgatggtaa atattcatat cgccaaaaat gaagcccgct
     5221 tgttgaatgc tgttaagaag cagttcaccg tttagctcgc taccgtgatg cgccgcgacg
     5281 ttcatgataa tcaccgcttc tttgcgcttc ggtttatcca taactggagc cggttccgct
     5341 acaggctcag gctgtggtgc cgctacgggt tctgcaggct ggaaagcctg ttgtgccggt
     5401 tgcggtgctg aatgcacagg ctgcggcgct ggcgcgacct gtggcgaaac tggctgctgc
     5461 aacggctgtt caggctgcgg ctgataggca ggctgctgca ccggctgcgc tggacgcgga
     5521 gcatgttgcg gcggtacctg cgcttcaggc ggctgctgga ccggttgacg cggctgcgca
     5581 gacgcataag gcggttggta ctggtgttgc ggcgacggac gagcagcctc atgctcctga
     5641 gcgttagccg gggcatgatt cacgcggtga acacgaacct caccaacgcc ctcatcatct
     5701 tcgacatcct cgtcataaga atcgtcgtca cgttttgact tcattcgttt taatggccga
     5761 tcgcggaaca tagaagatcg ttctttacgg ctggtccaga aaccatgtac cagtaaagcg
     5821 attatggcga tcgcgccaac aatgattaat atcagacgca aatcctgcat cattatattc
     5881 tctgttgttc taacaccttg ccaccacggc aaacatttac tcactaagag tatttgccga
     5941 ttacctcaag tgcaagtgca ctattaactt tcacagcaca aagatagatg aaatcgtgct
     6001 ttttgctgtt ttttcgaaca tatcctaact gtccattgcg caattacccg gtaaaatacg
     6061 cagaattttc ctgggattgg tcaaaaggag ctcatcctga ttatggtttc atcattcaca
     6121 tctgccccac gcagcggttt ttactatttt gcgcaaggct ggaagctcgt ctcgcaacct
     6181 gggattcggc gtttcgttat tttaccgctg ctggtcaata ttttgttgat ggggggcgca
     6241 ttctggtggc tctttacaca gctcgatgtc tggatcccga ctctcatgag ttacgttccg
     6301 gactggctac aatggctgag ttatctgttg tggcctctgg ctgtcatctc tgtgctgtta
     6361 gtgtttggct atttcttctc cacgattgct aactggattg ccgctccgtt taacggttta
     6421 ttggctgaac aactggaagc acgattgact ggcgctacac cgccagatac cgggattttc
     6481 ggtatcatga aagatgtgcc gcgaatcatg aaacgcgaat ggcaaaaatt tgcctggtat
     6541 ctgccgcgcg caattgtatt gctaattctt tacttaatcc ctggtattgg gcaaaccgtc
     6601 gcgccggtac tgtggttcct gtttagcgcc tggatgttag ccatccagta ttgcgattac
     6661 cccttcgata accacaaagt gccgtttaaa gagatgcgca ccgccctgcg cacacgcaaa
     6721 atcaccaata tgcagtttgg tgctttaacc agcctgttta cgatgatccc gctgcttaat
     6781 ctgttcatca tgcccgttgc cgtttgtggc gcgacggcga tgtgggtcga ttgctatcgc
     6841 gataaacacg cgatgtggcg gtaacaatct accggttatt ttgtaaaccg tttgtgtgaa
     6901 acaggggtgg cttatgccgc cccttattcc atcttgcatg tcattatttc ccttctgtat
     6961 atagatatgc taaatcctta cttccgcata ttctctgagc gggtatgcta cctgttgtat
     7021 cccaatttca tacagttaag gacaggccat gagtaagatt tttgaagata actcgctgac
     7081 tatcggtcac acgccgctgg ttcgcctgaa tcgcatcggt aacggacgca ttctggcgaa
     7141 ggtggaatct cgtaacccca gcttcagcgt taagtgccgt atcggtgcca acatgatttg
     7201 ggatgccgaa aagcgcggcg tgctgaaacc aggcgttgaa ctggttgaac cgaccagcgg
     7261 taataccggg attgcactgg cctatgtagc tgccgctcgc ggttacaaac tcaccctgac
     7321 catgccagaa accatgagta ttgaacgccg caagctgctg aaagcgttag gtgcaaacct
     7381 ggtgctgacg gaaggtgcta aaggcatgaa aggcgcaatc caaaaagcag aagaaattgt
     7441 cgccagcaat ccagagaaat acctgctgct gcaacaattc agcaatccgg caaaccctga
     7501 aattcacgaa aaaaccaccg gtccggagat ctgggaagat accgacggtc aggttgatgt
     7561 atttattgct ggcgttggga ctggcggtac gctgactggc gtcagccgct acattaaagg
     7621 caccaaaggc aagaccgatc ttatctctgt cgccgttgag ccaaccgatt ctccagttat
     7681 cgcccaggca ctggctggtg aagagattaa acctggcccg cataaaattc agggtattgg
     7741 cgctggtttt atcccagcta acctcgatct caagctggtc gataaagtca ttggcatcac
     7801 caatgaagaa gcgatttcta ccgcacgtcg tctgatggaa gaagaaggta ttcttgcagg
     7861 tatctcttct ggagcagctg ttgctgcggc gttgaaacta caagaagatg aaagctttac
     7921 caacaagaat attgtggtta ttctaccgtc atcgggtgag cgttatttaa gcaccgcatt
     7981 gtttgccgat ctcttcactg agaaagaact gcaacagtaa tgccagcttg ttaaaaatgc
     8041 gtaaaaaagc acctttttag gtgctttttt gtggcctgct tcaaactttc gcccctcttg
     8101 gcattgattc agcctgtcgg aactggtatt taaccagact aattattttg atgcgcgaaa
     8161 ttaatcgtta caggaaaagc caaagctgaa tcgattttat gatttggttc aattcttcct
     8221 ttagcggcat aatgtttaat gacgtacgaa acgtcagcgg tcaacacccg ccagcaatgg
     8281 actgtattgc gctcttcgtg cgtcgcgtct gttaaaaact ggcgctaaca atacaggcta
     8341 aagtcgaacc gccaggctag actttagttc cacaacacta aacctataag ttggggaaat
     8401 acaatgttcc agcaagaagt taccattacc gctccgaacg gtctgcacac ccgccctgct
     8461 gcccagtttg taaaagaagc taagggcttc acttctgaaa ttactgtgac ttccaacggc
     8521 aaaagcgcca gcgcgaaaag cctgtttaaa ctgcagactc tgggcctgac tcaaggtacc
     8581 gttgtgacta tctccgcaga aggcgaagac gagcagaaag cggttgaaca tctggttaaa
     8641 ctgatggcgg aactcgagta atttcccggg ttcttttaaa aatcagtcac aagtaaggta
     8701 gggttatgat ttcaggcatt ttagcatccc cgggtatcgc tttcggtaaa gctctgcttc
     8761 tgaaagaaga cgaaattgtc attgaccgga aaaaaatttc tgccgaccag gttgatcagg
     8821 aagttgaacg ttttctgagc ggtcgtgcca aggcatcagc ccagctggaa acgatcaaaa
     8881 cgaaagctgg tgaaacgttc ggtgaagaaa aagaagccat ctttgaaggg catattatgc
     8941 tgctcgaaga tgaggagctg gagcaggaaa tcatagccct gattaaagat aagcacatga
     9001 cagctgacgc agctgctcat gaagttatcg aaggtcaggc ttctgccctg gaagagctgg
     9061 atgatgaata cctgaaagaa cgtgcggctg acgtacgtga tatcggtaag cgcctgctgc
     9121 gcaacatcct gggcctgaag attatcgacc tgagcgccat tcaggatgaa gtcattctgg
     9181 ttgccgctga cctgacgccg tccgaaaccg cacagctgaa cctgaagaag gtgctgggtt
     9241 tcatcaccga cgcgggtggc cgtacttccc acacctctat catggcgcgt tctctggaac
     9301 tgcctgctat cgtgggtacc ggtagcgtca cctctcaggt gaaaaatgac gactatctga
     9361 ttctggatgc cgtaaataat caggtttacg tcaatccaac caacgaagtt attgataaaa
     9421 tgcgcgctgt tcaggagcaa gtggcttctg aaaaagcaga gcttgctaaa ctgaaagatc
     9481 tgccagctat tacgctggac ggtcaccagg tagaagtatg cgctaacatt ggtacggttc
     9541 gtgacgttga aggtgcagag cgtaacggcg ctgaaggcgt tggtctgtat cgtactgagt
     9601 tcctgttcat ggaccgcgac gcgctgccca ctgaagaaga acagtttgct gcttacaaag
     9661 cagtggctga agcgtgtggc tcgcaagcgg ttatcgttcg taccatggac atcggcggcg
     9721 acaaagagct gccatacatg aacttcccga aagaagagaa cccgttcctc ggctggcgcg
     9781 ctatccgtat cgcgatggat cgtaaagaga tcctgcgcga tcagctccgc gctatcctgc
     9841 gtgcctcggc tttcggtaaa ttgcgcatta tgttcccgat gatcatctct gttgaagaag
     9901 tgcgtgcact gcgcaaagag atcgaaatct acaaacagga actgcgcgac gaaggtaaag
     9961 cgtttgacga gtcaattgaa atcggcgtaa tggtggaaac accggctgcc gcaacaattg
    10021 cacgtcattt agccaaagaa gttgatttct ttagtatcgg caccaatgat ttaacgcagt
    10081 acactctggc agttgaccgt ggtaatgata tgatttcaca cctttaccaa ccaatgtcac
    10141 cgtccgtgct gaacttgatc aagcaagtta ttgatgcttc tcatgctgaa ggcaaatgga
    10201 ctggcatgtg tggtgagctt gctggcgatg aacgtgctac acttctgttg ctggggatgg
    10261 gtctggacga attctctatg agcgccattt ctatcccgcg cattaagaag attatccgta
    10321 acacgaactt cgaagatgcg aaggtgttag cagagcaggc tcttgctcaa ccgacaacgg
    10381 acgagttgat gacgctggtt aacaagttca ttgaagaaaa aacaatctgc taatccacga
    10441 gatgcggccc aatttactgc ttaggagaag atcatgggtt tgttcgataa actgaaatct
    10501 ctggtttccg acgacaagaa ggataccgga actattgaga tcattgctcc gctctctggc
    10561 gagatcgtca atatcgaaga cgtgccggat gtcgtttttg cggaaaaaat cgttggtgat
    10621 ggtattgcta tcaaaccaac gggtaacaaa atggtcgcgc ctgtagacgg taccattggt
    10681 aaaatctttg aaaccaatca cgcgttctct atcgaatctg atagcggcgt tgaactgttc
    10741 gtccacttcg gtatcgacac cgttgaactg aaaggcgaag gcttcaagcg tattgctgaa
    10801 gaaggtcagc gcgtgaaagt tggcgatact gtcattgaat ttgatctgcc gctactggaa
    10861 gagaaagcca agtctaccct gactccggtt gttatctcca acatggacga aatcaaagaa
    10921 ctgatcaaac tgtccggtag cgtaaccgtg ggtgaaaccc cggttatccg catcaagaag
    10981 taattcttgc cgcagtgaaa aatggcgccc atcggcgcca ttttttatgc ttccgccagc
    11041 ggcggcaaaa tcaattcatc gctctcatgc tgctgggtgt aacgcatcac ttccagtacg
    11101 cgcaaccccg cacggtgcac cgcatcgttt aacgccttcc ctttcagcaa accactgata
    11161 agctgagcac aaaacaggtc gccagtccct ttcaggtcgg tttttacccg tgaatgggaa
    11221 atgacattca cgctgtcggc actgaccacc accacctgca tctcctgatt ttcttcatta
    11281 ccggaggcgc tggtaatcac cacccatttt aatgtgtctg aaagcagact ttttgcggca
    11341 gcaatggcac tgtcgagatc gcggcaattt ttaccggtca ggatttccag ctcaaagata
    11401 ttgggggtaa ttccctgcgc cagcggcagt aaatattgtc gatacgcctc gggaaggtca
    11461 ggtttgacat aaattccgct atcaatatcg ccaatcaccg gatcgaccat gatcaatagg
    11521 tcaggatggt ctttgcgtag cgcagtcagc cactcggcaa ggattttgat ttgcgatgcc
    11581 gttcccatat agcccgtggt tacagcgcgc agctggcgca gcgcatcacg ctcctgaagc
    11641 gcacgcaaat aaccgctaaa ccattcatcc ggaatcgcac caccgtagaa agtgtcataa
    11701 tgcggcgtat tgctcagcaa taccgtcggc acggcaaaaa cattcagacc gttctgtttg
    11761 atagcaggca cggcaatgct gttgcccacg ctgccgtaaa ccacctgcga ctgcacggcg
    11821 acgatatccg cctgcagcgc cctgctctta tcgttaaaca acaacaaact actcatttaa
    11881 ttttttctcc ttgccgatga tcctcatcgt attccaaccg aaactttacc tgattctggc
    11941 agtcaaatcg gctatcacaa aacaaggata aggtaattca atgaagaaaa tcatttgtct
    12001 ggtcattaca ctactaatga cactccccgc ttacgcgaag ttaactgccc atgaagaagc
    12061 ccgcatcaac gccatgctgg agggattagc acagaaaaag gatttgatat ttgtgcgcaa
    12121 cggtgatgaa catacctgcg atgaagcggt ttctcatctg cgtctgaagc tgggcaatac
    12181 ccgtaaccgc attgacactg ccgagcagtt tattgataag gttgcttcgt cgtcatcgat
    12241 taccgggaag ccgtatattg tgaagatccc cggtaagagc gatgagaacg cacagccttt
    12301 tttacatgcg ctaattgcgc agacggataa aacggtgcct gcgcaataat cttaaatccc
    12361 cgccccctgg ctaaaatgct cttccccaaa caccccggtg gaaaggtagc gatcgccacg
    12421 atcgcagatg atcgctacca ccaccgcacc agggttagct tttgccaccc gcagtgctcc
    12481 ggcaaccgcg ccgccggagc tgacgccaca gaatattcct tcccgcactg ccagttcgcg
    12541 catggtgttt tctgcatcgc gctgatgaat atccagcacc tcatccacca gagaagcgtt
    12601 gaaaatcccc ggcagatatt ccgcaggcca gcggcgaatg ccgggaatac tgctgccttc
    12661 ttccggctgc aggccaacaa tcgttactgg tttggattgt tcgcgcataa agcgtgaaac
    12721 gccggtgata gtgccggttg ttcccatgct ggagacaaaa tgggtaatgc gcccgccggt
    12781 ttgctgccag atttccggcc cggtagtggt gtaatgggcg taagggttat cgggattatt
    12841 aaactgatcg agcagctttc cttcgccacg attcgccatc tccagcgcca gatcgcgcgc
    12901 accttccatg ccctgctctt tggtgacaag aatcagttcc gcaccataag cacgcatcgc
    12961 cgcacggcgt tcctggctca tgttgtcggg catcagcaat ttcatgcgat agcctttcag
    13021 cgcggcaatc attgccagcg caatgccggt gttaccactg gtggcttcga ttaagacatc
    13081 acccggttta atttccccgc gcttttccgc ctcgacgatc atcgaaagtg ccgcacgatc
    13141 tttcaccgaa cctgccgggt tattgccttc cagttttaac cacacttcac tgccgttatc
    13201 cggccccatt cgctgcaact tcaccagagg cgtattgcct attgtttgtt ctaatgtact
    13261 cacgatctct atccatacgt ggtgttgcct gatgcgacgc ttgcgcgtct tatcaggtct
    13321 acaggttaca aaccttgcca ttaaaaagcc cggatcgcgg gaagcgcctc cgggcgttta
    13381 acattcactc aacctatcag gcgctttgtg cgagagcaag ttcctcatct cgggtttcga
    13441 tacgctcgtc gccgttatac agccgcgcat gttgcagacc aacgaataaa cgctcgccac
    13501 gctgcggggc atcgtcgcca tgcatcacga ccgtcagcgg ttcgttgtac caccccagcg
    13561 gctgcaccac taattgggtg tagtgacctt tcgggctggc ttccagtacc tgtaccggca
    13621 gcggcgaatc gagactggta cggcggctga tatccacttc ccacgggcgc aggaagagat
    13681 ccaccggacc ctgatacgca ggtgtgtagc ccagcggcca gcgatgcgcg ccaacatgga
    13741 actgcccgcc gcgaatggtt ccctgcaggc ggttcacttc gcccataaat tcgagtacaa
    13801 aacgggtcgc tggttcgcgc catacctgat tcggcgcatc agcctgttca atattcccct
    13861 gactcatcac cactacacga tcggctactt cggtcgcctc ttcctggtcg tgggtcacga
    13921 acacgctggt gaatttcagc tcttcatgca gttgacgcag ccagcgacgc agctctttac
    13981 gcacctgcgc atccagcgcg ccaaacggtt catcaagcag cagaatttgc ggttctacag
    14041 ccagcgcgcg cgccagcgcc acgcgctgtt tctggccgcc ggaaagctgt gccggataac
    14101 gatctgccag atgagcaagc tgcaccatct ccagcaattt tgtcactttc gctttgatgg
    14161 ctgcggcatt cgggcgatcg cgacgcggca gcaccgtcag gccaaaagcg atattgtcga
    14221 ataccgtcat atggcggaac agcgcgtaat gctggaacac gaaaccgacc ttgcgatcgc
    14281 gcgcgtgcag gcggctgacg tcggtgccgt ggaagcgaat atgcccgctg gtttgatgct
    14341 ccagcccggc gataatgcgc agcagcgtgg ttttcccgga accggacggc cccagcaacg
    14401 cgaccatctg acctgaagga atatccagtg agatatcgtt cagcacctgg gtgcgaccaa
    14461 acgacttctt aatattggca atctcaatgc tcatgatgtt cctcctgctg tgcgcgtttt
    14521 tcctgattct ccaggcgcca ctgcaacata ctttttaaaa acagggtgat aatcgccatc
    14581 agcgttaaca gcgccgcagc ggtaaaggag ccgacggtgt tgtagtcctg ctccagcaat
    14641 tcaatctgta acggcagcga cagggtttcg ccgcgaatcg agccggaaac caccgacacc
    14701 gcgccaaact cgccaattgc gcgggcgttg gacaacacca cgccataaag cagcgcccag
    14761 cggatgttcg gtaatgtgac gcgacggaac atctgccagc cggacgcgcc aagcaaaatc
    14821 gccgcttcgt cttcctggct gccctggctt aacatcaccg gcaccagttc gcgcaccaca
    14881 aacggacacg tcacgaagat ggtgaccagc accattcccg gccaggagaa cataatttgc
    14941 aggttatgct cgtcgagcca accgccgagc gggccgttag agccgtagaa cagcaaatac
    15001 accagaccgg caaccaccgg cgacacggca aacggaatgt ccagcagcgt cagcagcaac
    15061 tggcgtccgg ggaagttaaa gcgcgtcacc agccacgcca gtagaatacc gaacaccagg
    15121 tttaccggta cggcaatcag cgcgatcatc accgtcagcc agatggcgtg cagcatatcc
    15181 ggatcggcca gattctgtaa aacaggcatc agccctttgc tgaacgcctg tacgaagata
    15241 taaatcatcg gcaccagcag gatgaacgcc gataccagca tcccgatgcc aatcagaaac
    15301 catttgcccc agttaatcgg gcgcgcgtca taacgcttca attgtgtcac ttccgccatt
    15361 aatgacctac cacacgccga ccaaagcgac tttgcagagt gttaattgag aacagcagta
    15421 tcagggatgc cgcgaggatc accgaagcaa tcgcgctcgc cgccgggtaa tcaaactcct
    15481 gcaagcgcac aaaaatcatc agcgacgtca cttccgtctt ccacgcgata tttccggcga
    15541 taaaaatcac cgcgccaaat tcaccaagac tacgggtaaa cgacagcgcc acgcccgcca
    15601 ccagcgccgg agaaagctcc ggcagcacca ctttgcagaa actctgccag cgcgttgcac
    15661 caagcgtttc cgccgcttct tcatattccg ggcctaactc ttccagcacc ggctgcacgg
    15721 tacgcaccac aaacggaatg ctggtaaagg ccatcgccac cgcaatcccc agccatgtat
    15781 aggtgacttt gatatcaaac ttcgccagcc attcaccgta aaaaccgttt acggaaaaga
    15841 gcgaggccag cgttaaaccg gcgacagccg ttggcagcgc aaagggtaaa tccatcagcg
    15901 catcaagcag cgtgcggcct gggaagcgat agcgggttag gatccacgcc atcagcagac
    15961 cgaaaacgcc gttaaaaatc gatgccacaa acgccgacag cagcgttact ttgtaggccg
    16021 cgaccacctg cgggttggtg atcacctccc agtactgcgc ccagctcatc tgggccagtt
    16081 gcatcaccag cgcggagagc ggcagcagca aaatcaggca cacaaacagc agactggtgc
    16141 cgaggcttaa ggtaaagccc ggcagcacgc gtctggagga gacagcaaac atcagttacg
    16201 ccccgccgct aacagcttgt ctaactcgcc gccgctggtg aagtgggttt tcatcacttc
    16261 cggccaggag ccaaatttgt cttccacgcg gaacagctcg gtctgcggga atttgtcttt
    16321 cagtttgtcc atcacctccg ggttattcac gcggtagtaa tagtcggtga tgatggtttg
    16381 cgcctgcggg ctatagagcc agttcagata ggctttggcg gctttttccg taccgttggc
    16441 ctgcacgttt ttatcaaccc acgccaccgg gaattccgcc agaatgttgg ttttcggaat
    16501 caccacttca aagccctgcg cttcatactg tttacggatg ttgttcactt ccgattcgaa
    16561 gctaatcagc acatcgccca ggccacgctc ggcaaaagtg gtggttgcgc cacgaccgcc
    16621 ggtatcgaac acttcaacgt ttttcaggaa ctgggtcata aactgttcgg ttttggcttt
    16681 atcgccaccg tcagctttat ccgctgcgcc ccatgccgcc agataggtat aacgcgcgtt
    16741 acccgacgtt ttcgggttcg ggaaaatcag cttcacgtcg gagcgcacca ggtcgttcca
    16801 atcgtggata ttcttcgggt tacccttacg caccaggaag cccatggtgg agtagaacgg
    16861 cgagctatta ttcggcaggc gcgactgcca gtcggccggg atcagcttgc ctttatcgtg
    16921 caggatttgt acgtcggtca cctggttata agtgacaacg tcggctttta agccctgtaa
    16981 aatcgccagc gcctgttttg atgacccggc atgagattgt tttatcgtca gtttgtcgcc
    17041 gccgttatct tttgcccatt gttgctcaaa cggcggattc agggcggcaa acagctcgcg
    17101 ggagacgtca taagaactgt tcagcagttc cgttgcctgt acatggcccg ccagcagcag
    17161 agaagcgacc agcgcgagtg agttcttttt cagtaagtta acggccattg cgcaccctta
    17221 taaatttaat gactttctaa tagccatcat atttataacg gacgtgaaag gagtaacggt
    17281 tttatatacc gtttggtgat ttggaagttg aaaagggaat aagaaccggg tttaaaacga
    17341 aatcctcccc acaaatgcag ggaggaaaca gaggtgaatc agataccgac gctaaccgtc
    17401 tccggcagtg tgctgccgcc atcaatcaca ttctgtgtac cggttaaata gctggattca
    17461 tccgatgcga ggaaggccgc cagttcgccg acttccagcg gatcggcgag gcgacgcatc
    17521 gggattgctt tcgccatttc agtcagcacc gactctggat cttccgggtt cgactggcgg
    17581 gcaatgcttt ccgccattgg tgtgcgcacg tatcccgggc aaatggcgtt aacgcgaata
    17641 ccagactgcg cgtactccac cgccagcgat tttgtcaggc caacaatcgc cgctttcgtt
    17701 aaggcgtacg ccgtttcgcc aggatcggcc accatatcgc cagtgaccga agacatcatc
    17761 acaatgcgac catctttgcg ggcaatcatc tccggcagca ccgccttcgt gacgttccat
    17821 acgcctttaa tattgatgtc aatatggaaa tcgcgatcgt catcgctcat atcgaggaaa
    17881 ctgcccagac gacaaacgcc tgcgttattc accaggatat caatgcgccc ttctttttcc
    17941 ttcgcgcgtt tgatagctgc ggctaccgac gccgggtcac gcacatcggc gacaaccgcc
    18001 gtacagcgat gaccacgacc acacagttcg tccgccagct tttcgatctc aggggagata
    18061 tccagcaaga ttaggtttgc gccatgacgc gcaaaagttc tggcaattcc ttcgccaatt
    18121 ccctgcaatg cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc
    18181 tccttgttaa tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata
    18241 ttgctgcgga tcagctcact aattcaggaa aatctcaatc cagtcgctgc tttatgcatc
    18301 atttcaggcg ttgtgttagc tcactgctgc gctgaatcat tttcaatgac tccacatcat
    18361 tgagttgcac aagaccaaca aacagtaaat cggtaacaga attttgcgcc gtgcgggtag
    18421 acatcgacga gctacgccat tcggtttcac cggaaacggt atcgagcgtg aaatgcgcca
    18481 gccgccgcag cggagaatca gccagagaag taatggcaat caccgttgcc ccctgctttc
    18541 gtgccgcttc tgcgcataag acaatctctt ttttactgcc gctgtaagag atagcgattt
    18601 gtacatcgcc ttttttcaac gcctgggaaa cggtggcttg tacgtgcgtg tcggcttcgc
    18661 aggccacgcg atagccaatt ttcatcagtt tgaacgacag atcgcgcccc accagcgccg
    18721 atccgcccag gcccgttatc tggataaacg gtgctttact gatgacctca atgatttttt
    18781 gcagccgcgc gtagtcgaac aacgcacagg tttgctccag cgccagctct ttttcgcgat
    18841 tcaatttgcg ggcaatcact tccagcgaat catcactggt aatcgaactg tgcaggtgca
    18901 gcgccgtggc attcgttttc tcccggctgg cactgtattc gcctattaac gccatacgca
    18961 attcagtaaa tccttgcgcg ccgagttttt gggcaaattt cactatgctc gactggctaa
    19021 tgccgagctg tttcgccatc tgccgtgaag aaacggattg cagctcactc acgttggcct
    19081 gcagaaagtc agcgattttt tgctcgtttt cggtgaattc actccccgca ttgcttattt
    19141 tcgtcaggta caacatcttc cactctcctg ggctggcagc ataaattccg cttaagttct
    19201 tatgacgctc ttcacactct gcgagtgtag actcaatgat tcctttaagt ctgtcaattc
    19261 gggatatatg attccataca tctgcaatta tttgatcgta aatagtaagg tcaccaccga
    19321 tgcaactaga aaagatgatt actgaaggct cgaacaccgc ctcggctgaa attgaccgcg
    19381 tatcgacgct ggaaatgtgc cggattatca acgatgaaga taaaaccgta ccgcttgccg
    19441 ttgagcgcgt cctgccggat atcgccgcgg cgatcgatgt tatccacgcc caggtcagcg
    19501 gcggcggtcg tctgatttat ctcggtgcgg gaacatccgg tcgtctgggg attctggatg
    19561 ccagcgaatg tccgcccacc tacggcgtga aaccgggtct ggtggttggt ttgattgctg
    19621 gaggcgaata tgccattcag cacgcggtgg aaggcgctga agatagccgg gaaggtggtg
    19681 ttaacgatct gaaaaatatt aatttaacgg cacaggatgt ggtggttggc attgctgcca
    19741 gcggtcgcac gccgtatgtg attgccggac tggaatacgc acgccagctc ggctgccgca
    19801 cagtgggaat ttcctgtaat ccggggagtg ccgtttccac caccgccgag tttgccatta
    19861 caccgattgt aggtgccgaa gtggtcactg gttcttcgcg aatgaaagca ggcacagcgc
    19921 agaaactggt gctcaatatg ctttccaccg ggctgatgat taaatctggc aaagtgttcg
    19981 gcaacctgat ggtcgatgtg gtcgccacca acgaaaaact gcatgtgcgc caggtcaata
    20041 tcgttaaaaa cgccaccgga tgtaatgccg aacaagcgga agcggcgtta attgcctgcg
    20101 agcgcaactg taaaacggcc attgtgatgg tgctgaaaaa tctcgatgcc gcagaagcta
    20161 aaaaacgcct ggatcaacac ggcggcttta ttcgtcaggt tttagacaag gaataaccca
    20221 tggccaaaga gatcagcagt gaacttctga acaccattct tacccgtgtc ggcggaccgg
    20281 gaaatatcgc cagttgtggt aactgtatga cgcgcctgcg tctgggtgta catgacagtt
    20341 cactggttga tcccaatatc aaaacgctgg aaggcgtgaa gggcgtcatt ttgaccagcg
    20401 accaggtgca ggtcgttttt gggcctggta aagcgcatcg tgccgcaaaa gcgatgagcg
    20461 agctgctggg cgaggcaccg gtacaggatg ccgcagaaat cgccgcccag aacaaacgtc
    20521 agttaaaagc caaacaaacc tccggcgtgc aacaatttct cgccaaattc gccaccatct
    20581 tcacgccgct gatccccggt tttattgccg ccggtctgct gctggggata gcgacgttaa
    20641 ttgccacggt gatgcacgtt ccggcagatg ctcagggcac gctacccgac gcgctgaatt
    20701 ttatgaaggt gttcagcaaa ggtttgttca ctttcctggt gattctggtg ggctataacg
    20761 ccgcccaggc atttggcggc acgggcgtca atggcgcaat tattgccgca ctctttttgc
    20821 tcggttataa ccccgctgca accaccggtt actacgccgg ttttcacgat ttctttggtc
    20881 tgcccatcga tccgcgcggc aatattatcg gcgtgctgat tgccgcgtgg gcctgcgccc
    20941 gcattgaagg catggtgcgc cgctttatgc cggacgatct cgacatgctg ctgacctcgt
    21001 taatcaccct gctgatcacc gctacgcttg cgtacctgat cattatgccg ctgggtggct
    21061 ggttattcga aggtatgtca tggctgttta tgcacctgaa cagtaatccg ttcggttgtg
    21121 cggttttagc cgggctgttc ctgatcgccg tggtatttgg cgtgcatcag ggctttattc
    21181 ctgtttacct cgcgttaatg gacagccagg gattcaacag cttatttccc atcctttcaa
    21241 tggcaggcgc gggccaggtg ggcgcggcgc tggcactcta ctggcgggcg caaccgcaca
    21301 gtgcattacg cagtcaggta cgcggggcga ttattcccgg tctgctgggc gttggtgaac
    21361 cgttgattta cggcgtcacc ctgccccgca tgaaaccgtt tattaccgcc tgtttaggcg
    21421 gcgcagcggg cggtttgttt atcggcctga tagcctggtg gggtctgccg atgggcttaa
    21481 acagcgcctt tgggccgtct ggtctggtgg cgctgccgct gatgacttcc gcacaaggca
    21541 tccttccggc aatggcggtt tatgctggcg ggattctggt ggcatgggtt tgcgggttta
    21601 ttttcaccac gctctttggc tgccgtaacg tcaatctgga ctgatatcat gaaacggaca
    21661 atgctctatc tttctctgct ggctgttagt tgtagcgtca gcgccgcaaa ataccctgtt
    21721 ctgacagaaa gttcgccaga gaaagcaggg ttcaacgtcg aacggcttaa ccagatggat
    21781 cgctggatta gccagcaagt ggacgccggt tatcccggcg taaacctgct gatcattaaa
    21841 gataatcaga ttgtgtatcg caaagcctgg ggagcggcga aaaagtacga tggcagcgtg
    21901 ttgatggaac agcctgtcaa agccaccacc gggacgctgt atgacctggc ctcaaacacc
    21961 aaaatgtacg ccacgaactt cgccctgcaa aagctgatga gcgaaggcaa actgcatcct
    22021 gacgatcgga ttgcgaaata tattccgggg tttgccgata gtccaaatga caccattaaa
    22081 ggcaaaaaca ccctgcggat ttctgacctg ctgcatcaca gcggcggttt cccggcagat
    22141 ccgcaatacc cgaataaagc ggtcgcgggc gcgttatatt cccaggataa aggccagacg
    22201 ctggagatga tcaagcgcac gccgctagag tatcagcccg gcagcaagca tatttacagt
    22261 gatgtcgatt atatgctgct tggatttatc gtcgagtcgg tcaccggtca gccgcttgac
    22321 cgctatgttg aagagtcgat ttatcgcccg ctcggcctga cacatacggt atttaacccg
    22381 ctgctgaaag gctttaaacc gcaacaaatt gccgccacgg aattgaacgg caatacccgc
    22441 gatggcgtga tccatttccc gaatatccgc acctccactc tctggggtca ggtgcacgat
    22501 gaaaaagcct tttattcgat gggcggcgtt tccgggcacg caggtttgtt ttccaatacc
    22561 ggcgatattg cggtgttaat gcaaacgatg ctgaacggcg ggggctatgg cgatgtgcag
    22621 ctgttcagtg cggaaacggt gaagatgttc accaccagct ctaaggaaga tgccactttt
    22681 ggcctcggct ggcgcgtgaa tggtaatgcc accatgacgc cgacgtttgg cacgctggca
    22741 agcccgcaaa cctacggtca cactggctgg accggaacgg tgaccgttat cgatccggtg
    22801 aatcatatgg cgattgtgat gttaagcaac aagccacatt cgccggttgc cgatccgcaa
    22861 aagaatccca atatgttcga aagcggtcag ctgccgattg caacgtatgg ttgggtagtt
    22921 gatcaggtgt atgcggcgtt aaagcaaaag taataaaaag gccagtcagt aatgttactg
    22981 cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcaacttgt ccagcgacgg
    23041 tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc aacatcgcat cacgcttacc
    23101 atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta
    23161 ggcgcagaag tacagaccgt gagtgccact ggcagtgccg tacggcaggc tctggcgaac
    23221 aatcttcagc cctttgccat cttctttcag atcaacgcgg gtgaggtgag aggtttccgg
    23281 acgttcgtcg ccgtcgatct cttcgttggc ctctttggtg cgcccgatca tcatctcctg
    23341 atcgtgaacg ctcatccggt tgagctgctt caggttgtgt tcccaacgct ggacaaacac
    23401 atagctgccg cccgcatcca cgccgtcttt gataaccgcc acttcgcgac gcgtctcttc
    23461 acccgccggg ttttccgtac cgtcaacaaa gccgctcagg tcacgctctt caacccaacg
    23521 gaagccgtgg atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg
    23581 ggcgacagag aagtttacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg
    23641 ggtggtcggc gcaaggcctt taccgtagcc cggaaaatct ttcagctctt ctgccccaac
    23701 gccgccgctc agagcgcgcc aggtgttgtt accaaaggca accaccgcac caagatgcgc
    23761 gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg
    23821 cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc
    23881 tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataata atgccacgca
    23941 atccgtggca ttgttgagcg ctattttacg tgttttttcg cggcggcggt ttgttcagac
    24001 gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa
    24061 ggcattaacc cttcaggacc actccattcc ccgctgaact ggtagctaat atgttgactg
    24121 ccttcggctt tacattcgac ggcacgatta tcatcaccat cagctttttg gcaattgcca
    24181 aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt taacaccggt gtcagcagga
    24241 atatcgctat ccagcacgtc aatgcgactg atcgtgcctt gatcgccgtt aatcaccatc
    24301 gccacgttgt cgcccttcat cacttcaaaa aagcgcacca cgttgccgtt cgcggttttc
    24361 attccgctgc gcaggcgata atcgccatca agcgcatcgg caatggcttg ttcttgcagt
    24421 ggtgtggacg ccgttaattc accaacgccc tgctcgctca ctttggtgga cgacccaaac
    24481 cagttccacg ggttagcggc agaccagtta actgacgaca tcgtggaaca gccggtcagc
    24541 atcaatggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt
    24601 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt tacttcatta
    24661 aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacaat atccgccact
    24721 accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccag
    24781 caaagtaaaa ccacctgcgc caacagcaac agaaaataga gtacacgcca ggtgcgagga
    24841 aacgtagccc gccgaccgct caacaaaaac gccagcaccg ccggaatgcc aggaatcagc
    24901 cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctca
    24961 cgcgacgcac cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc
    25021 cagaagagca agggtaaacg caggcgtccg tgcgcatcat aatggacagg atggaactca
    25081 gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc
    25141 caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacattga tctgaatttt
    25201 cgggcagcca cgagcaatca gctttttctc cagccgatta agcaacgcat tggcaatccc
    25261 acgcccacga aactctggat gcacgccaag ataataagca gacccgcgat gcccgtcata
    25321 accgcccatc accgttccga ccacttcacc gtttacctca gcgaccaaaa acaaactgac
    25381 gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac gcagcaagtc
    25441 gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc
    25501 catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt
    25561 agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg
    25621 aaatgaaaag taaaacaatt ctcaacatca aaccgtcgta acggattacg cgatacgata
    25681 taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa
    25741 acactcactt cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga
    25801 atgtcgcaag ccatcgccaa agacgaacct ttaaaaacca gcaacggaca cagcaagccg
    25861 aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg atccaggcca cggcggaatt
    25921 gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcgattgct
    25981 aaaaacgtcc gttccatttt gcgtaatcat gggattgatg cgcgtttaac gcgttctggc
    26041 gatacgttta tcccacttta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg
    26101 tttatgtcaa ttcatgccga tggctttacc aacccgaaag ctgccggtgc ttcggtattt
    26161 gccctctcta accgtggggc aagtagcgca atggcgaaat acctgtctga acgcgaaaac
    26221 cgcgccgatg aagttgccgg taaaaaggcg actgacaagg atcacctatt gcagcaagtg
    26281 ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgctcgg ctcgcatatt
    26341 ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt
    26401 gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg aaacctcgtt tatcaccaac
    26461 ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc agaaaatcgc cacagcgatt
    26521 gctgaaggcg tgatcagtta tttccactgg ttcgacaacc agaaagcaca ttcgaaaaag
    26581 cgataagtta tgaaacccga cgcacaccag gttaaacagt ttctgctcaa ccttcaggat
    26641 acgatttgtc agcagctgac cgccgtcgat ggcgcagaat ttgtcgaaga tagttggcag
    26701 cgcgaagctg gcggcggcgg gcgtagtcgg gtgttgcgta atggtggtgt tttcgaacag
    26761 gcaggcgtca acttttctca cgtccacggc gaggcgatgc cagcttccgc caccgctcat
    26821 cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg
    26881 cataacccgt atgttcccac cagccacgcg aatgtgcgtt tttttattgc cgaaaaaccg
    26941 ggtgcagagc ccgtctggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt
    27001 gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggtgaa
    27061 gacgtttatc cccgttacaa aaagtggtgc gacgattact tctacctcaa acatcgcaac
    27121 gaacagcgcg gtattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac
    27181 tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta
    27241 gagcgacgta aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc
    27301 ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca ctctgtttgg cctgcaaact
    27361 ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat
    27421 tatcagccaa aagatggcag cccagaagcg gcgttaagtg agtttattaa ggtcagggat
    27481 tgggtgtaac tccctcaccc ccattcccgc atccgctgat gcaacgtcaa cgacggcttc
    27541 tcggcaaaca gctgctggta atccgtggca aattgcccca gatgccagaa tccccactgc
    27601 atggcggcgt cttttaccgt tgtgctttgc gaccacgggc ttatcagttc gcggcgtacg
    27661 gcgttcaggc gaatgcgttt cagccacgcg tttgggccaa tgcctaaaat agcgtgaaac
    27721 gcgttttgta gcgtgcggcg actgacatgc agttgattac acaagtccag caccgtcacc
    27781 ggctcagaca tattttccag cacatattca cgggcgcggg aaagtaatct acggtaactc
    27841 tgatgactga tgctttccgc cgtcaccatt ggttgcgcgt cttccagcat cgcccccatc
    27901 accattagca aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg
    27961 ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagcgcggc tttatgctgc
    28021 tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc cgggttatgc
    28081 aaaaagttag cctgccgggt gatgacatct tcagaaagca ccacgcccag gatcgtgtaa
    28141 tcatccggcg tgctcagttc aaattcagtg ccaccagggc gggtggcgat ttccgcgctt
    28201 cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat gccaaaccag
    28261 aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccggtgta ttcacgaaaa
    28321 acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc
    28381 tgatcgtaaa tctgctgcca ggcggtaatc gttaaagcat gttcatagac atccgttgtc
    28441 tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag
    28501 ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg
    28561 ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg ataacgacta
    28621 ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc
    28681 agcggccagc caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt
    28741 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccctt
    28801 acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    28861 cgaccgatct ccttgcggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    28921 tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttaa gcgaccaggg
    28981 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca
    29041 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    29101 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    29161 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    29221 tcagacggtg gcgggacata ggtcaccagc tgtacgtcgg cagatttcaa cgctgcatca
    29281 atgccatagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    29341 atcccggcgg ttgatgagag ataagaaccg gtacgcgaaa ccacatgcgc caggaaggcg
    29401 gtgttttctg cgtcattggc ccactggaat gctgcaccat tttcaatatg tgcaaccatc
    29461 gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca
    29521 ccggcagtcg gtgacgggcc gtgtgcagcg cccgcataca gcgagcgacc atataccact
    29581 tccaccatcg cctgcttggt cgcttcatca gcggcaatgt aggtgacgtc atcggaatca
    29641 gcagaaatca gcccgaggct acgaatatgc ggcggcaatt ttagttcacg cgcaaattca
    29701 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    29761 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    29821 ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg
    29881 ttagagatac aggtgcgatc ggcctcaacg gtggtcgcca tacgcggcga gtaaacggcg
    29941 tagcaggaga ggctttctga ctgccctaac cccggacgtt cccccaccag caggatcacc
    30001 actttcgcgc cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgaaca
    30061 aagaatggcg tgccgacttt cagcccggcc tgtttcaggc ccgccatcag cggcggcagg
    30121 atctcttcgt agttcacggt gatggcatca gttgacaggc catcagaaat aacgacctgt
    30181 acgtccggat tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg
    30241 cggcccatat ccgggcgagt caggtagaga tttttgtcgc taatttcaga gcgtacttcc
    30301 agcaagccct gcgctttcac ccactcttcc ggcacttctt tcagtacggt atctttcgaa
    30361 cgagagtgat cggccaggaa acgcagcagc gcctgggtac gtggacgcgg accggcacga
    30421 ccggtacaaa cgcgggccac ggtgctgcgg cgcagttctg ttaatacgtc tgcgcgatgc
    30481 gggttttcaa caccaatcca cgcttttgct tcagcggaac ctaaatccag cgcacagctt
    30541 tccgaggtca ccggtgccgc acagttggtg gtggcgcact ttgcttctga cggcgcgggg
    30601 gccgtttgtc ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc
    30661 atgatgtgtt atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca
    30721 ggcgaccgtt tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacggac
    30781 gcaggttgag taactgacgc acagtggcgg tatcgtggaa tgcggtggtc tggtagttga
    30841 gcatgatgtc atcacccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca
    30901 ggatcatcag gttttcgttg aggttctggt cagcgtcagc gtggttggtg taacagcagt
    30961 cacagcccat agagatgccg ctcagcttgc ccataaagtg atcttctaag ccagcacgga
    31021 taatctggcg gtcgttgtag agatactccg gcccaataaa gccgaccacg gtgttgacga
    31081 taaacggatc gtaatgacgc gccagcccat agttacgtgc ttccatcgtc acctggtcag
    31141 cgccgaagtt agcgccagcg gatagcgcag agccttgtcc ggtttcgaag tagaggcagt
    31201 tttccccggc gatacggttg aactctgcac ccactgcgcg cgcttcgtcg agcatcgcca
    31261 gttccacgcc aaactctttc agcccttttt cgctgccaca gatactctgg aaaatcagtc
    31321 cgccaggcgc gccgcgacga atcgcttcga tctgggtggt gacgtgcgcc agtacgcagc
    31381 cctgagttgg gatgttgaat ttgtcgatca cgccgtaaat ggtgtccaac acgcggctta
    31441 agttttccac gtcgtcagtc accgggttaa cgccgatcac cgcatcgccc accccgaagg
    31501 aaagcccttc gtagatttgc gcggcgatac tttgcacgtc gtcacgggtg tcatttggct
    31561 gcaaacgggc gctgaaggtg cccggaatac caatggtggt attggccttt ttgattaccg
    31621 gcattttctt cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcga
    31681 ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc gtccacgctg gtttcatcgc
    31741 tcagcacata ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt
    31801 cgttaacatc gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat
    31861 tattgcggat gtccgctacg gtcatttccg acaacacctg ctttgccgcc acgcgctcct
    31921 gtgaacttgc cgctgcaacg cctgccagca catcccccga acgcagttcg ttggctttag
    31981 ccagcacctc ttttacatcc ttaaactgat atacattgct gaacaatgtg gtctttagtt
    32041 tcataagtcg ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga
    32101 tccgccaaaa agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc
    32161 aatgactgcc aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc
    32221 aaagtcctgc ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc
    32281 gaccagcgcg ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag
    32341 cgccagcacg tacgcgtcag ttttgggatc aagatccagc tgaagcagcg cctgttgcca
    32401 ggcactcacc agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag
    32461 ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc
    32521 aatcaccgtg gcgcgtacgg tttgcgccgg aaactgcaca ttcatctcac gcaggcgcgg
    32581 atggtcatgc agcgccgttg ccagaagtgg gccaatatcg gcaaaacaga acgggtcggc
    32641 gggctggtgg cgataacatt cgcccacgcc tccagaaagc gtaatgattt cgggcgtaac
    32701 acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaaa gcgttccgtc
    32761 aatcacttcg acaatcagcg ccgccatccg ccgggtaacc tgcaccagct gcgcgccggt
    32821 cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc acaatcatct gccccggttt
    32881 atgagcgtaa accacgcgcc cctggctgtc ggtttccagc aggcgaccac cgacgttgag
    32941 acaggcagtg ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc
    33001 gatgtcgata ttcagtaccc gacacagccg ttgttcagaa agggtttgtg ccccggctcc
    33061 gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg gcaacgacaa aatcgcccag
    33121 cgactgagag agcgccatca ccgccgggcg agcattgcgg gttttcgcgc tttcaccggt
    33181 gatgatgatg gcaccagaat caacgctttc cggcgcaatg cccgcagcct gatattgctc
    33241 taaaatcagc gatttcagtt ccgcttcttt taaaccaccc tgtttatcga caggggtaaa
    33301 gaataccggg ctttgccagc taatttcgcg tttaatgaat tcgtagcgcg gcacctgcga
    33361 caccgccgca cggttaacca gttccaggtg ggagaaaatc acctgggtgg tggtggtgcc
    33421 gatatcgata ccgacgctca atagctggcg agtgttcacg attgtgcctc cgcttcggtt
    33481 ttagtcgcgg tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat cgccgttacg
    33541 ccgccgatca acttgccgac aatcatcggg aagatcatgg cgttcatgtt ggcagcggcg
    33601 aagcctaagt ggtcgcccag ggcgaaagca gcggaaacgg cgaaggcgca gttgatgact
    33661 ttgccgcggg tatccatctg cttcatcatg ccgaacatcg ggatgttgtt ggcaagcgtt
    33721 gccaccatgc cggctgccgc gatgttgttc atattcagta ctttaccgac gctcatcagc
    33781 ggtttttcaa accagcgagt cagcagcagc accattggat acgcccctaa cagcacgcag
    33841 gagatagaac cgataacttc aatggcgcgc atcacttcac cgggtttatc gccaggggcc
    33901 ataaagatag gatccagtcc ggggatcagt tcccagccaa ggaggaattt cactaccgca
    33961 gcggcaagac cgagggtgat caatgcaacg aggaatttgg caaagatctg gaagccgttg
    34021 atcatttttt ccgggatgaa tttcagcccc agcgccacca gaatcgcgac aatgatcacc
    34081 gggatcatgt tcatcaggat cagggcgaaa gtgaattcca ccggctgacc gttgatctgg
    34141 acaccggagt acatagcaac cagaccacca gcaatacagc caatcggaat ggtcacaatg
    34201 cccgccagca cgccgagcgc cagataacga cgatcagaag gttcgataat gccgagcgcc
    34261 accggaatgg aaaacacaat cgttggcccc atcatcgacc cgagaattaa cccagagtat
    34321 agccacgctg ctacgtcgcc gcccgccagc tctttggcga ggaagaagcc gcccatatcg
    34381 cacgccagca gtgttccggc gaacattgac gggtttgcgc cgagcatttc gtaaaccggg
    34441 ataattaccg gcccgagtac gtgagccagt accggtgcca gcgcggtcat accgaccatc
    34501 gccagaccca gagcgcccat tgccataaag ccttcttcga actgaccgcc tgatccttcg
    34561 atacttttac cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg
    34621 tctacggcag ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaattccc
    34681 atcgccttta ctccctgtta gttgttattt attggcggat gcggcgtaaa cgccttatcc
    34741 gccctacatg tgcaatcccg taggccggat aagacgcggc aagcgtcgca tccggcattt
    34801 attgcgccgc cgcgtacagt ccgacaatct gttccaggct ggcggtacgc gggttgctgc
    34861 gcagacaaat atcttcctgc gcggcctgcg cccatgcacc gtaatgcgca gatgtcgcgc
    34921 caacatcgcc cagtcgttta ccaatcccaa cttccgcaat cagctcactt accgcgttaa
    34981 tagcgtcacg atcgtcggat tttttagttc gcagtgcccg accaatctga ctaaaccgtt
    35041 cgcgacaaac catccggtta aattccatca ccgttggcag caacatggcg ttcgccagac
    35101 cgtgtggaat atgcagcgcc gcgcccggct gatgcgccat cgcgtggcac aacccaagac
    35161 ccgcactgga aaacgccatt cccgccatac atgaagccag caacatgctc tcgcgcgcgg
    35221 caaggtcgtg accgtagccc accgctttcg gcagcgattt gccaatcatc gcaatggcac
    35281 caatcgccag actgtcggta aacggtgtag cgttcagggc gctgtatgct tcaatggcat
    35341 gggttaacgc atcaatgccg gtcatcgccg tgacatgcga cggcacacct tcggtcaatg
    35401 cggcgtcgag gatcgccaca tccggcatca gcgaggcatg ggctaacacc tgcttgcgcc
    35461 cgctcaccgc gtcgataatc accgttacat tggtggtttc agagccggtt ccggcggtag
    35521 ttggaatggc aatcagcggc aagcgcggtt gcagaacgct ggtttctgac atctctgcca
    35581 gcgtgctatc cgggttcgtc accagcaacg ccacggcttt cgccgcatcc agcaccgagc
    35641 cgccgccaaa tgcgatcacc ccgtcacaac ctgactcacg caactgcgcc acggctgcac
    35701 acacgtcggt aatgcacggt tcgcccaccg gacatagcca gagcgtcatg gcgatgcctt
    35761 taacggccag gctgcgcgtc agcccggcgg tcatccctgc ctgatgcaaa aagctgtctg
    35821 ccatcacgaa cagatgtttc agcccacgcg tttgcgcttg ctgcccgcaa ctgctcaccg
    35881 cgcccagacc gcaaagcgtc accggtggaa cgctaaatgt ttttacccgt tgcagattca
    35941 gggtatcgaa cgcctgaaag agcgcggtct gcaattcatt ttgcatatag cccctccgct
    36001 ttctctcgcc cactactggc gatcgccagc ggggtcataa acaaactgtg ctgcggtaaa
    36061 tgcacctgta acgccgggaa ttgtttgcga aacagttctg ccacgcccgg ttgcatacag
    36121 gagccgcccg ccagccataa atcagtaatg ccctgccctt caatatgccg ggcgacaatg
    36181 tccgccattt tttcgtacac cggtttcacc gcaggccaaa tctcgtcacc gtgaccgcgc
    36241 ttgtactgct ccgcctcttc cagcgaaata cggcgatttc cggcgagagt cagagagatg
    36301 tgatgtccgc cggtggcttc atccgccgag tacgtcactt tgcccttttt cacgatggca
    36361 atgccggtag tgccaccgcc gatatccact acgcctgcgt tatccagttg cagtagatcc
    36421 gcgactgcgg ttggctcatc cagcacatgg ctcacttcca gcccggcaga ctccagcacg
    36481 ttgatggaaa tacgcgggtc cgtacctgga ggaaatgaag tcgccgcatg gctaaaacgg
    36541 cgaccgaatt gctgctcgag cgtgtcgaga tggcgacgaa caatggtgac agcgccgaag
    36601 aaatcccaga cgatgccgtc gcggaccaca tcggcccagt cgaggcatac cgccaccggc
    36661 tgaccgtcgc ggtcgacaac catcgacacc acatcgcagg tgcccagatc cacccccagc
    36721 cacagcggag attccgtcgc ggcgggcgtc tggttacaca gcgttgccgc cgtttgcaga
    36781 cgtggggtga gccattgttc gtcgtgcgcc atctgttact ccttaaacaa tgcgaaacgc
    36841 atcgactaat acacagcgac gcagacggac aaacgtacgc gcgctggtta ccccttcacc
    36901 ggttggcgtg gtgatggtca tggtggtcca gccttccccg cccagcccca gcccggcaat
    36961 gcacggtccg ttcttaacga aaatgctggt atcaatagca ttcgccatct ggttcatgtt
    37021 ttcgatgttg cgcgagtgca ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc
    37081 cagcgcaatg gcatccgcca cgttggcgac gcgcacgacg ggcagcaccg gcatcatcag
    37141 ttcggtcacg gcaaacggat gttctgcagt ggtttccaca aacagcaaac gcgtttcttg
    37201 cggcacgttc aggccgattg ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg
    37261 gctgacggtg ccttttccgc gctcgtcgat atttttcagc aacaccggtt gcagctgctg
    37321 cgcctgttct gcggtcagtt tcaccgcgtg ctggccttcc atcagacgca tcagttcatc
    37381 ggctacgcta tcaacaacaa tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa
    37441 agacgcgcct ttgacgatgg actgagcagc acgggcgagg tcagcggttt catccaccac
    37501 taccggcggg ttgccagcgc ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc
    37561 ttctactacc gcttcgccgc cggttaccac cagcaggccg atacccggaa acttgaacaa
    37621 gcgttgcgcg gtttcgatat ccggatttgc cacagtaacc agtaagtttt ccggcccacc
    37681 tgcggcaaca atcgcctggt tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc
    37741 cggatgcggg gcaaaaatga cgctgttgcc cgcggcaatc aggctgatgg cgttattaat
    37801 tacggttgcc gccgggttag tggacggagt taccgaagcc accacgcccc agggcgcgtt
    37861 ttcaattaaa gtcaggccgt tgtcgccggt cagcacctgc ggagagagac actcaacgcc
    37921 tggcgtgccg cgcgcctgag cgacgttttt ggcaaattta tcttcaacgc ggcccatgcc
    37981 ggtttcactg acggcaagtt ccgctaaatc tctggcgtgt ttttcgcctg cctcacgaat
    38041 ggcagcaatg gctaactggc gcattgccac gctttttaac ccttgctggg cgactttggc
    38101 tgccgcaacg gcgtcatcca gggacgcgaa aacgcccatc tcatgaacgg cggcggacgg
    38161 cgtgtcactg ctttgcattt tcagcagtac cgctttcacc acctgttcaa tatcctgttg
    38221 attcatgatg ttctgcctta tttgtggaaa attacctgac cgccagacac cacctcatcg
    38281 acaatgccaa tcacgcacag atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg
    38341 gaactgccac tcaccagtaa cacccactcc ccggttcccg cgccaatatt gtcgatggcg
    38401 acggcgcatt gcccgtcagg attaccttgt ggatcaatca tttccaccat cagcaatttg
    38461 tcatgcgcca gtccgtgatg gcgtacggta caaacaattt gtccagtgac gactgccagt
    38521 ttcatacccg cctccgtggc gtatttcagg taaaagctcc ccctaccctc cgcagaaggt
    38581 aaaatgaaaa aggagagagc gtgacgcccg aatcgacgtc acacagggtg attacaggtt
    38641 gctgctatcg cctttcaggc cgatcgggaa gacttcttct aggtcaccgt gcggacgcgg
    38701 gataacgtgt acggatacca gctcgccaat acgctgtgcc gctgcggcac cggcatcggt
    38761 cgccgcttta caggctgcaa catcaccgcg aaccatggca gtacacaggc cgccgccaat
    38821 ctgcttaaca ccaaccagct tgacgcgcgc agctttcacc atcgcgtcag aagcctcaat
    38881 cagtgcaacc aggccccggg tttcgatcat tcctaatgct tccattgtgc tttcctcttt
    38941 atcagggtcc agaacgggac cgttcattca accagtgttt gtaaactgct ttcgcggttc
    39001 acttttgtct gacgcggcac agctgccacc agcgccagct cgataatttc ctgcacgcta
    39061 caaccacgag agagatcgtg catcggcgcg gcaagtcctt gtatcaatgg cccgacggca
    39121 cgatatccgc cgagtcgttg tgcgattttg tagccaatat ttccggcttc cagcgacgga
    39181 aaaaccatca cattggcctt gccctgtagc gggctggcag gcgctttttg cgccgccact
    39241 tccggcacga aggcggcgtc aaactgtaac tcgccatcaa ccaccagctt tggtgcgcgc
    39301 tcacggacga tttctgtcgc ctgctggacg ttggcaacgc aggggtgacg ggcgctaccg
    39361 ttactggaaa acgacagcat cgccacgcgc ggctcttctc cggtgatggc gcgccaggtt
    39421 tcggcactgg caagcgcgat atccgccagc tgcgccgccg tcggctgtgg caccacgctg
    39481 caatcggcaa agcccaacgc cgggccgctg tactgtggca acatcaggaa aatggaagag
    39541 agcgttttac agcctggctg caagccgata atgcgtaatc cggcacgcag cacatttgcc
    39601 gtggaagaga ggttgcccgc gatacagaca tccgctttac cggcgctgac cattgctgcg
    39661 gcgaacatca gagggtcggt aagtttttcc agcgcatccg gcggcgtttt ttcgcccgcg
    39721 cgggccagcc agcgatgagc aaattcttcc cgcattgcga ggttgccatg cggatcaatc
    39781 acctgtagcc cgtccatcgc cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga
    39841 ttggcgacca gaatgggcgt tgccagacct tgttgatgta aatattgcgc ggctttcagc
    39901 acacgttggt ctaacgcatc cggaaaaacc actctggcgg gcgctcgcaa cgccagttca
    39961 cgacaacgtt caataatcat tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta
    40021 gcggctgctt cgttacgctc aggatcatca tcacgtagac cgtgctcgaa aggcggttca
    40081 gcgcctgcaa aatgtccggg cgtaatactt caaagctgcg ggtgataaag acctgcgccg
    40141 ctacggtttc ggtttcccgc actttggtgc gtagcagatt gagcaacgca gcatcgcgcc
    40201 cgtggctggc ttccggcacc agatgatcgt ggtcgagata gcgcagcggc tgatgagaaa
    40261 gccgatgcag atcttcgtca gaaagcccga caatcgcctg gcagcccagc ggttccccca
    40321 gcgcatcggc gcgcataatg ttgccaagac gcgaacggat atccgccagc cacggctgcc
    40381 acggttccgc cagttcaatt tgcagccaca ctgccagcgc aatggtgctg tcgagaaccg
    40441 cgcgaaaacc cagacgcgga tcgcttttgg cgaccatttt ttccgccgac aggtgagtca
    40501 gcgtatccgg cttttttgcc accggctggc ggcacagttc gcagcacgcc tgcggatgtt
    40561 catcgctact ggtcagccca tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca
    40621 ggcggccctg ctcgtcaata aacttgatgc gcagatggcg gctttccagc aactcccggg
    40681 cagagggcgt caggcgactg tccgcaggga gatggatctc tgcgccttcg ctgagcgtat
    40741 ggttcgctct tagccatgct tcggtgatga aatctttcat agggattgcc agttagccgg
    40801 ccaggcgaca tacaggaatt tcacgctgga tgtcgtacca aattcgatgc tggagccttt
    40861 cgggataaac atcacgtcgc ctgctttggc gatcatggtt tcgccttcgt ggcgaacatg
    40921 tagctcccct tccagcacca tgtcgatttc gtcgtagttc agggtccacg ggaagaatgc
    40981 gttctcccac tgcataaaac cggcggccat gctgctgccg tcgtctccgg tcacaagatc
    41041 agtcaagccc acgcaatgcg gctctgcgcc gtcgaaacga ccaaacttga cgctgctgcc
    41101 atcaatcact ttgatgccgc ctttgccggt caccgatttg aagctcggct gcattgcgcc
    41161 ctgctccagc gactgttttt ccttcatcac tttttccatc agctgtgcga ccaggctttc
    41221 ggtaaactgg ccttccggca gctgggcaat gatggtttcg cggatgcgct ggctttcggt
    41281 tttgtcggct ggcacgctgg cgggcacgga cgccgttacc gggatcgatt catcacattc
    41341 ggtgatggta aaacccagta gatccgccac ttcacgggct tccggggtaa taatgctggc
    41401 gcgcagaacc acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc
    41461 tgtgatgagt tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca
    41521 gttgctgcac gctttgcggg tcgtggctgt ttagctcgaa aatcggctct tcgaaacccg
    41581 tctcaagcaa taactttcgc gttgcggcga cgtcagcatc tggcatgtcc gttttactga
    41641 tgacggcgat ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc aaccgacttt
    41701 ccgggtcatt tgcgccatgg acatagatca gcatgtctac atcctgtagc gtggtaatta
    41761 aggcgtgata ccagcgggga tggctaaaat attcacccgg cgtatcaata tcgcccttat
    41821 cattaaattc cacggcctgt gtttttctgg cgagggtata attcccctgc aacgcattaa
    41881 ataacgttgt ttttcctgcc ccgacgctac ccacaaacgc aattcgtttc atgaatgcct
    41941 cgtattaact tttggtcatt tcacaaagcg tataatttaa taagcgacct aaaccgctga
    42001 cggtttgtga taacgcttct tctactgcgc ccaccgagcc ataaatcacc aatgcaccgc
    42061 tgaagcggtc gagaaaaccg atatgcacat cagcggcttt cagcgctaaa tcaccggcga
    42121 tcatcgccgt ttcgccggga gttagcgtca taatgccaat tgcgcccgca tccggaacgc
    42181 cgattttctt cgccagttcc tcaccgggat gcgcgatgag atgcgccagt gtgacctgtt
    42241 tgccgggcac aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa
    42301 ctttcgtgta tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcgcgttt
    42361 aaaaggtgag gtagatcact aagaatggag agatattcgc tgtggtgcat aaactttcat
    42421 atgcaacgca tgaatataaa taaaataata tcgagatatt atatagactg ctttttttat
    42481 tattcgcaaa ctcagtaata agctttacta atatattgca gccaaaccgc ttaccctgaa
    42541 tattcagggt aagcgtgaga gttaaaaaag aattacagcg gttgggtttg cgcttcaacc
    42601 acggccagcg ccaccatgtt gacgatacga cgcaccgatg cgatcggcgt taacacgtga
    42661 accggtttcg ccacgcccat cagcaccgga ccgacagtca caccttccga gctggaaacg
    42721 cgcagtaagt tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattggca
    42781 gaacctttca aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca
    42841 tcgccgtgca tttcaccatc aatcatcaga tctggcgcac gatccctgac cagttccagc
    42901 gcctgacgca ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac
    42961 agagcaacgc gcggctcaat accaaaacga cggacagttt ctgccgccat caaggtgatc
    43021 tccgccagct cttctgcgtc tggttcgtca ttgacgtagg tatcggcaat aaaggtgtta
    43081 ccactcggca gcagcagcgc gttcattgca cctgcggtgt gaacgccatc gcgataacca
    43141 aagacatttt tcaccacgct aaaatgttca tgataatcgc ccaccgtacc gcagatcatt
    43201 gcatcggctt ccccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc
    43261 gcccgctgcg cctgttcctg agtgacaccg cgacgcttca tgatctggaa gtattcggtc
    43321 cagtactctt taaagcgcgg atcggattcg ttattgacga tctcaaaatc aacgcccgct
    43381 ttgatctgca agcccagttt ctgaatgcgc atttcgatca cgttcggacg accgataagg
    43441 atcggtttcg ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct
    43501 tccccttctg gcagaacaac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc
    43561 ataaacaggt tggttttgta aacgaactca gtcagcttgt cgatgtagac gtcgaaatca
    43621 gcaatcggac gcgtcgccac gcccgactcc atcgcggctt tagcgaccgc aggagcgatc
    43681 ttaacgatca agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga
    43741 tcctgatcgc catacgctga agccaccact tcgctctgtt cggcgtgggc cagttccgca
    43801 atcgcgcgta ccgccgccag tttcatctct tcattgatag ccgttgcgcc aacatccagc
    43861 gcgccacgga agataaacgg gaagcaaagg acgttgttga cctggtttgg atagtcagaa
    43921 cgaccggtgc aaatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc
    43981 ggttccgggt tcgccagcgc caggatcatt ggcgcacgag ccattttctt caccatttcc
    44041 tgggtcagca ctttcgggcc ggaacatcca aggaaaatat ccgcgccttc aatcacatca
    44101 tcgagggtac gtttgccgtc atccaccacc gcatatgcgg ctttggtttc cgccatgttt
    44161 ggctcacggc cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc
    44221 agacccagcg ctaccagcag gttcatacag gcgattgctg cggcacccgc gccggaaacc
    44281 accatccgca cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca
    44341 gtgctgatga ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg
    44401 cgcagtttct gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgattccg
    44461 ccgaaggtcg gttcgagcgc agcgacaact tcaataaatt tgtccgggtc gagttcatca
    44521 acttcaatgt caaatacatc aatcccggcg aatttcttaa acagaacgcc cttgccttcc
    44581 atcaccggtt tgccagccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta
    44641 gagatcaccg ccaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt
    44701 tcgatttcaa gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc
    44761 gttgccagag gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa
    44821 tcaagtgcac tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac
    44881 aaataaagtg tgtgggcaac agcccctgcc cacaacgtgg cgcacattat taccctgccg
    44941 gagtctacag actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa
    45001 tcctataaca ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt
    45061 tttgatgcgt ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta
    45121 cttttcaagg agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg
    45181 tggcagacag cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca
    45241 atccttcgct gttactcaaa gctgccggat tatcacaata tgagcattta atagacgatg
    45301 ctatcgcctg gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg
    45361 acaaactggc agtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa
    45421 cagaagttga tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc
    45481 tggtggactt gtatcagcaa caaggcgttg agaaatcacg cattttgatc aaactggctt
    45541 cgacctggga aggaattcgc gcggccgaag agctggaaaa agaaggtatt aactgcaacc
    45601 tgacgctgct gttttctttt gcccaggcgc gggcctgtgc agaagcgggc gtttttctga
    45661 tttcgccgtt tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atggacccgt
    45721 atgtggtgga agaagatccg ggcgttaaat cggtgcgcaa tatctacgac tactataagc
    45781 aacaccacta tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg
    45841 ccttaaccgg ctgcgatcga ctgactatcg caccgaattt gctgaaggag ctgcaggaaa
    45901 aagtttcgcc agtggtacgt aaattaatcc cgccttctca gacgttccca cgcccagcac
    45961 ccatgagcga agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac
    46021 tgtctgaagg cattcgtcta ttcgccgttg atcaacgcaa actggaagat cttcttgccg
    46081 ccaaactata aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt
    46141 cgcgcactca gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg
    46201 ggcatggctg atattgccga agtgctgtgg aacgattttc ttaaacataa tcctaccgac
    46261 ccaacctggt atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc
    46321 tacagtttgc tacatctgac cggttacgac ctgccgctgg aagaactgaa gaacttccgt
    46381 cagttgcatt cgaaaacccc aggccacccg gagattggct atacgccagg cgttgaaacc
    46441 accaccggcc cgcttggaca aggtttggcg aacgccgtcg ggctggcgat agcggaacgt
    46501 acactggcgg cgcagtttaa ccagccagac catgagatcg tcgatcactt cacctatgtg
    46561 tttatgggcg acggctgcct gatggaaggt atttcccacg aagtctgttc gctggcaggc
    46621 acgctgggac tgggcaagct gattggtttt tacgatcaca acggtatttc catcgacggt
    46681 gaaacagaag gctggtttac cgacgatacg gcaaaacgtt ttgaagccta tcactggcat
    46741 gtgatccatg aaatcgacgg tcacgatccg caggcggtga aggaagcgat ccttgaagcg
    46801 caaagcgtga aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg
    46861 ccgaataaag caggtaagga agaggcgcac ggcgcaccac tgggggaaga agaagtggcg
    46921 ctggcacggc aaaaactggg ctggcaccat ccgccatttg agatccctaa aaagatttat
    46981 cacgcctggg atgcccgtga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt
    47041 gccgcctata aaaaggctca tccgcaactg gcagaagagt ttacccgacg gatgagcggt
    47101 ggtttaccga aggactggga gaaaacgact cagaaatata tcaatgagtt acaggcaaat
    47161 ccggcgaaaa tcgctacccg taaggcttcg caaaatacgc ttaacgctta cgggccgatg
    47221 ctgcctgagt tgctcggcgg ttcggcggat ctggctccca gcaacctgac catctggaaa
    47281 ggttctgttt cgctgaagga agatccagcg ggcaactaca ttcactacgg ggtgcgtgaa
    47341 tttggcatga ccgctatcgc caacggcatc gcgcaccacg gcggctttgt gccgtatacc
    47401 gcgacgttcc tgatgtttgt tgaatacgcc cgtaacgccg cgcggatggc ggcactgatg
    47461 aaagcgcggc agattatggt ttatacccac gactcaattg gcctgggcga agatggtccg
    47521 acgcaccagg ctgttgagca actggccagc ctgcgcttaa cgccaaattt cagcacctgg
    47581 cgaccgtgcg atcaggtgga agcggcggtg ggctggaagc tggcggttga gcgccacaac
    47641 ggaccgacgg cactgatcct ctcaaggcag aatctggccc aggtggaacg tacgccggat
    47701 caggttaaag agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat
    47761 attattctga ttgccaccgg ttcagagatg gaaattaccc tgcaagcggc agagaaatta
    47821 gcaggagaag gtcgcaatgt acgcgtagtt tccctgccct cgaccgatat tttcgacgcc
    47881 caggatgagg aatatcggga gtcggtgttg ccttctaacg ttgcggctcg cgtggcggtg
    47941 gaagcaggta ttgccgatta ctggtacaag tatgttggtc tgaaaggggc aattgtcggg
    48001 atgacgggtt acggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc
    48061 gccgagaata ttgtggcaaa agcgcataag gtgctgggag tgaaaggtgc ctgatggtga
    48121 tcgccggatg ctgattgccg gatgcgacgc tgacgcgtct tatccggcct acagcgtctt
    48181 atccggccta catgtccccg caattttgtt taacgggtga tccacaacgt gggccaggcg
    48241 tctggcccat gccagttatc gcaggtgggt tctgcggcat aacgcaccag gcgaaaacgc
    48301 tgaccgtcaa agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct
    48361 aaggtcacca gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc
    48421 gtctcctgac cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaggc tagtggcttt
    48481 ttacgcgaca caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc
    48541 atcatcagcg ctttatcatc agtcagcgca gtgacattca cctcacgacg taacggatca
    48601 agcgagcagc gcagaccatt catccgccag tttccataat ccagcaaatc gttgcgttct
    48661 tcgagtgaga gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg
    48721 ggcggtacgc tgagcggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg
    48781 cgcttttgct gagcatcaat aaacaacaac gccgctttta agccactcag agaaatggtc
    48841 tgattgccgt cgcgtaaggt gatcgccttc ccttcctgaa tcatctgcaa aaacgcggtg
    48901 atggttgccg tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt
    48961 aacgccaacg gctcaccatc taacagcagc cgtggcgcta tctctccttc agacgcatcc
    49021 ggcgacttca acccgccgcg ctcaatacgt aaaacggcat cggtatgcgc cccggcgctg
    49081 cggctcaggg tcatcaccag tccattatga tcgcccgtat tgcgcgccac gcagcagttt
    49141 tggttattac aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc
    49201 aaagacgtcg gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt
    49261 acgaccccag aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg
    49321 ctcaatcaga tttgtcggat tgagccaata ataaacaacg aaatattatt gttatttttc
    49381 agtccattaa atgtgacgtt tgcaaatagt taagcaataa caccgtctta ccgtcacgta
    49441 tctcgccggt tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat
    49501 cttcatcttc aacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa
    49561 aaaagtggat tagctcagtc acaccgccag gcgacatata cagttcaaat aatttgcgca
    49621 cttcgcccac ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac acttccggtt
    49681 cgtcgttatc cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa
    49741 cccaggtagc gacacggaac tgacgaatca gaaccacggt ctttttcttc gcgttgtaca
    49801 ggaggatcgt cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc
    49861 catctttgcg cgtgagatcg taagtaatgt tgtgcagggt gaaatagtta tcggagagaa
    49921 ttttgtcttt aatgagggtg atttgttgcg tcatagcgac tccacagcgc gaaatgaaca
    49981 attatcttac gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat
    50041 tttaccccca gccagtcaat aatgccctgt gccgcatgac gtccttctgc cattgcggta
    50101 accaccagat ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc
    50161 tggtaacggt actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca
    50221 ccgtgcgact ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca
    50281 tcggctggca tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca
    50341 tccggctcgc caagacgcgt gcgcaggaaa cgaattccgc aaacgtgacc ttgttcattc
    50401 agctcaagcg ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc
    50461 ttcacctctt tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg
    50521 ttactcgcgc cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc
    50581 agtaccacga cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc
    50641 cccatcacct gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgca
    50701 tcttcattgg gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg
    50761 tcgtattgtt ccaaaagcga atccaaagag acatctttac ccacttcaca attgagttcg
    50821 aagtgaatcc ccatcgcgct gaagatttcg cgacggcgtg ccagcaggga tttatccagt
    50881 ttgaaagaag gaatgccgaa agtgagcaag ccaccaattt ctggatggcg atcgtacacc
    50941 gtcaccgcca cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cgggcctgcc
    51001 ccgataatcg ccacccgctt gtccactttg gtgacatgac ttaagtcagg acgccaacct
    51061 ttcgccaacg cctgatctga aatatagcgt tcaatgttgc cgatagttac agcaccgtgc
    51121 tcatcgcgaa tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta
    51181 atttccggca gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct
    51241 ttcaccagtt caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg
    51301 ctgtgctcgc cgcacttaag gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat
    51361 ggcagataaa tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg
    51421 ccacgcgcgg gcgttgcctg catttgttcg actttactca ttaccggcat ttcttgcgcc
    51481 gcggtactgg catgccacgg ttgatgttcc tgacgcgcgg tgcgcaagcg gcgggatttc
    51541 gccatgccgg agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg gcaattctca
    51601 acacaggcag gaccgttttc gcgccccgca caaaggtcgc atttatgcgc cgtggctttt
    51661 acttttcctg ccgcgacggg tgtcaggacg atttgcatcg taccaaaagg acaggccacc
    51721 acgcaggatt tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg
    51781 ctgattgcgc cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc
    51841 actgcactac gttgctgttg atgtttgata accgtaattc ggggatgaaa atggtgttgg
    51901 ctcaggacat gttgctcatc attgtgagcc atgacacagg cgatttcaca agcatgacaa
    51961 cccagacatt gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt
    52021 tgtaaaaacc ttattcttta tatgagtgtt gttattaccc gacttacagg ggaatcggca
    52081 atgttcatgt gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc
    52141 ccatcactga ctaaattgtg tttcagctaa ctggaacatt aatgattttt tgtggagaag
    52201 acgcgtgtga ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt
    52261 gtgctgctgt cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc
    52321 gacgctgagg ctatcaatat tgccggatcg ctacgtatgc agagttaccg cctgggctac
    52381 gacttgcaaa gtggcagtcc acaactcaat gcacatcgcc agttgtttca gcaggcactg
    52441 cattcaccgg tattaaccaa tctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc
    52501 tatgcgcatc tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg
    52561 ccgtggtatc aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct
    52621 ttacagcact acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggt
    52681 atcggtattt tcacgctggt cttttttact ttgcgccgta tacgccatca ggtggttgcc
    52741 ccgctgaatc agctggttac cgccagtcaa cgtattgaac acgggcagtt cgactcgccg
    52801 ccgctggata ccagtctgcc gaatgagctt ggtctgcttg caaaaacctt taaccagatg
    52861 tcgagcgagc tgcataaatt gtacctttcg ctggaggcgt cagtagaaga aaagacccgc
    52921 gatctccacg aggccaagcg tcgtctggag gtgttgtatc aatgttccca ggcgctgaac
    52981 accagccaga ttgatgtgca ttgtttccgc catattttgc agattgttcg cgataatgaa
    53041 gcggctgaat atctggagtt aaatgtcggt gaaaactggc ggattagcga agggcaacca
    53101 aacccggaat tgccgatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa
    53161 ctgcactggc aaaatagtca cgtttcatca tcagaaccgc tgcttaacag cgtttcgtcg
    53221 atgctgggac gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg
    53281 atggaagaac gtgcaaccat cgcccgcgaa ttgcacgact cgctggctca ggtactttct
    53341 tacttacgta tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca
    53401 caaagtatca tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag
    53461 ctgttgacta ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa
    53521 atgctggata cgttacaaaa tcaaaccagc gccaaactga ccctcgactg ccgtctgcca
    53581 accctggcgc tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg
    53641 ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg
    53701 gacggcaatc acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa
    53761 cccgaaggtc attatggtct gaatatcatg cgcgaacgcg cggaacggct gggtgggacg
    53821 ctgacttttt cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgcg
    53881 gagggtgagg aaagtcagct gatgtaatgc ctcctactga ccaaagaata cttgcactta
    53941 aggttcagta taaaagggca tgataattta cattaactcc tttttttctc cacgattggc
    54001 tcgtaccttg ccgctacagt gaagcaagtc aagcctacaa cgatacgcag aaacacgagg
    54061 tcctctttta atggcgaatt tctttattga tcgccccatt tttgcctggg tgctggcaat
    54121 cctgttgtgt ctgacaggta ccctggcgat tttctcattg cccgttgaac aataccccga
    54181 tctcgcgcca ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct
    54241 ggaaaacacc gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctcatgta
    54301 tatgtcatct cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg
    54361 caccgatccg gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa
    54421 gttaccgcag gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaatat
    54481 tctgaccatt gctttcgtct ctaccgatgg ttcgatggat aaacaggata ttgccgatta
    54541 tgttgccagt aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc
    54601 ctatggttcg caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca
    54661 gatgacggct aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg
    54721 gcaacttggt ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca
    54781 gtcactgctg caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaagacgg
    54841 ctcagaggta aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta
    54901 tcttagccgc ttcaatggta agccagcctc cgggctgggg gtaaaactgg cctccggcgc
    54961 aaacgaaatg gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtactt
    55021 cccgcacgga ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat
    55081 tgaagacgtg gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct
    55141 gttcctgcaa aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat
    55201 gggaaccttc tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc
    55261 gatggtgctg gcgatcggtc tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga
    55321 acgtattatg agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca
    55381 gatccagggg gcactggtcg ggattgcgat ggttctttcg gcggtatttg taccaatggc
    55441 cttcttcggc ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc
    55501 gatggtgctg tcagtactgg tagcgatgat cctcactccg gctctgtgtg ccacactact
    55561 taagccactg aaaaaaggtg agcatcacgg gcaaaaaggc ttttttgcct ggtttaacca
    55621 gatgtttaac cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag
    55681 cctgcgctgg attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg
    55741 tttgccgacg tcgttcttac cgctggaaga ccgtggcatg tttactacct cggtacaatt
    55801 gcccagcggt tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta
    55861 cttcacccat gaaaaagaca acatcatgtc ggtgtttgcc actgttggtt ctggccccgg
    55921 gggtaacggg caaaacgtgg cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga
    55981 cagtaagacc ggcacctcgt ttgccattat cgagcgtgca acaaaggcgt ttaacaagat
    56041 taaagaagct cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc
    56101 gggttttgat atggagttgc aggaccacgc tggagcaggt cacgatgcgc tgatggcagc
    56161 acgtaaccag ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa
    56221 cggcctcgac gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct
    56281 gggcgttgat atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt
    56341 taatgacttt atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta
    56401 tcgcatgctg ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt
    56461 gcccttctct gctttcgcga cctcacgctg ggagacaggc tcgccgcgtc tggaacgcta
    56521 taacggttat tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac
    56581 ggcgatggat attatggaat cgttagtgaa gcagttgcca aacggctttg gtctggagtg
    56641 gacggcgatg tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgctat
    56701 ttccttgctg gtggtattcc tgtgtctggc ggcgttgtat gaaagctggt cggtgccgtt
    56761 ctcggtaatg ctggtcgtgc cgctgggggt tatcggcgcg ctgctggcaa cctggatgcg
    56821 cgggctggaa aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc
    56881 gaaaaacgcc atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccacgacct
    56941 gtttgaagcg acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct
    57001 ggcatttatc ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca
    57061 gcatgcggtg ggtactggcg taatgggcgg gatgatttcg gccactattc tggctattta
    57121 cttcgtgccg ctgttctttg tactggtgcg ccgccgcttc ccgctgaagc cgcgcccgga
    57181 ataagcaata aaaaaggcga catgctaatg tgtcgccttt ttcaactttc cgattaagaa
    57241 cctgctcagc gggttcttgc tgtttgtact ttgtctcagg aattacttac gaagcataac
    57301 ttcgataaag tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat
    57361 tatgggtatt ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat
    57421 gactacctcc gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat
    57481 gtgatacaca gcaaca
//
LOCUS       DAPHJE010000009        55347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPHJE010000009 DAPHJE010000000
VERSION     DAPHJE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.009"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(56..268)
                     /gene="cspA"
                     /locus_tag="RH222_000623"
     CDS             complement(56..268)
                     /gene="cspA"
                     /locus_tag="RH222_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV3795688.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(549..839)
                     /locus_tag="RH222_000624"
     CDS             complement(549..839)
                     /locus_tag="RH222_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV3795689.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1274..1984
                     /locus_tag="RH222_000625"
     CDS             1274..1984
                     /locus_tag="RH222_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV3795690.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2034..3008)
                     /gene="ghrB"
                     /locus_tag="RH222_000626"
     CDS             complement(2034..3008)
                     /gene="ghrB"
                     /locus_tag="RH222_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV3795691.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3112..3771)
                     /locus_tag="RH222_000627"
     CDS             complement(3112..3771)
                     /locus_tag="RH222_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV3795692.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3925..6258
                     /gene="bisC"
                     /locus_tag="RH222_000628"
     CDS             3925..6258
                     /gene="bisC"
                     /locus_tag="RH222_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV3795693.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6227..6667)
                     /locus_tag="RH222_000629"
     CDS             complement(6227..6667)
                     /locus_tag="RH222_000629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3795694.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6664..7227)
                     /gene="tag"
                     /locus_tag="RH222_000630"
     CDS             complement(6664..7227)
                     /gene="tag"
                     /locus_tag="RH222_000630"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV3795695.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7385..8083
                     /locus_tag="RH222_000631"
     CDS             7385..8083
                     /locus_tag="RH222_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3795696.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8312..9520
                     /locus_tag="RH222_000632"
     CDS             8312..9520
                     /locus_tag="RH222_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3795697.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9844..11535
                     /gene="eptB"
                     /locus_tag="RH222_000633"
     CDS             9844..11535
                     /gene="eptB"
                     /locus_tag="RH222_000633"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV3795698.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11627..11703
                     /locus_tag="RH222_000634"
     tRNA            11627..11703
                     /locus_tag="RH222_000634"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11661..11663,aa:Pro,seq:cgg)
     gene            11738..11871
                     /locus_tag="RH222_000635"
     ncRNA           11738..11871
                     /ncRNA_class="other"
                     /locus_tag="RH222_000635"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12617..14224
                     /gene="dppA"
                     /locus_tag="RH222_000636"
     CDS             12617..14224
                     /gene="dppA"
                     /locus_tag="RH222_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV3795699.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14532..15551
                     /gene="dppB"
                     /locus_tag="RH222_000637"
     CDS             14532..15551
                     /gene="dppB"
                     /locus_tag="RH222_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV3795700.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15561..16463
                     /gene="dppC"
                     /locus_tag="RH222_000638"
     CDS             15561..16463
                     /gene="dppC"
                     /locus_tag="RH222_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV3795701.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16474..17457
                     /gene="dppD"
                     /locus_tag="RH222_000639"
     CDS             16474..17457
                     /gene="dppD"
                     /locus_tag="RH222_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV3795702.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17454..18458
                     /gene="dppF"
                     /locus_tag="RH222_000640"
     CDS             17454..18458
                     /gene="dppF"
                     /locus_tag="RH222_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV3795703.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18488..19759)
                     /locus_tag="RH222_000641"
     CDS             complement(18488..19759)
                     /locus_tag="RH222_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV3795704.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20235..20342
                     /locus_tag="RH222_000642"
     CDS             20235..20342
                     /locus_tag="RH222_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3795705.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20718..20825
                     /locus_tag="RH222_000643"
     CDS             20718..20825
                     /locus_tag="RH222_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3795706.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21201..21308
                     /locus_tag="RH222_000644"
     CDS             21201..21308
                     /locus_tag="RH222_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3795707.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21684..21791
                     /locus_tag="RH222_000645"
     CDS             21684..21791
                     /locus_tag="RH222_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3795708.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(21878..23557)
                     /gene="bcsG"
                     /locus_tag="RH222_000646"
     CDS             complement(21878..23557)
                     /gene="bcsG"
                     /locus_tag="RH222_000646"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV3795709.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23554..23745)
                     /gene="bcsF"
                     /locus_tag="RH222_000647"
     CDS             complement(23554..23745)
                     /gene="bcsF"
                     /locus_tag="RH222_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV3795710.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23742..25313)
                     /gene="bcsE"
                     /locus_tag="RH222_000648"
     CDS             complement(23742..25313)
                     /gene="bcsE"
                     /locus_tag="RH222_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV3795711.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25586..25774
                     /locus_tag="RH222_000649"
     CDS             25586..25774
                     /locus_tag="RH222_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV3795712.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25786..26538
                     /gene="bcsQ"
                     /locus_tag="RH222_000650"
     CDS             25786..26538
                     /gene="bcsQ"
                     /locus_tag="RH222_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV3795713.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26535..29153
                     /gene="bcsA"
                     /locus_tag="RH222_000651"
     CDS             26535..29153
                     /gene="bcsA"
                     /locus_tag="RH222_000651"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV3795714.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            29164..31503
                     /gene="bcsB"
                     /locus_tag="RH222_000652"
     CDS             29164..31503
                     /gene="bcsB"
                     /locus_tag="RH222_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDV3795715.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31510..32616
                     /gene="bcsZ"
                     /locus_tag="RH222_000653"
     CDS             31510..32616
                     /gene="bcsZ"
                     /locus_tag="RH222_000653"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV3795716.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32598..36071
                     /gene="bcsC"
                     /locus_tag="RH222_000654"
     CDS             32598..36071
                     /gene="bcsC"
                     /locus_tag="RH222_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV3795717.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            36153..38141
                     /gene="hmsP"
                     /locus_tag="RH222_000655"
     CDS             36153..38141
                     /gene="hmsP"
                     /locus_tag="RH222_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV3795718.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            38324..39610
                     /gene="dctA"
                     /locus_tag="RH222_000656"
     CDS             38324..39610
                     /gene="dctA"
                     /locus_tag="RH222_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV3795719.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            39831..41327
                     /locus_tag="RH222_000657"
     CDS             39831..41327
                     /locus_tag="RH222_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV3795720.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(41423..42352)
                     /gene="kdgK"
                     /locus_tag="RH222_000658"
     CDS             complement(41423..42352)
                     /gene="kdgK"
                     /locus_tag="RH222_000658"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV3795721.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            42584..43351
                     /gene="pdeH"
                     /locus_tag="RH222_000659"
     CDS             42584..43351
                     /gene="pdeH"
                     /locus_tag="RH222_000659"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV3795722.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            43421..45481
                     /locus_tag="RH222_000660"
     CDS             43421..45481
                     /locus_tag="RH222_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV3795723.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(45715..47037)
                     /locus_tag="RH222_000661"
     CDS             complement(45715..47037)
                     /locus_tag="RH222_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV3795724.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(47448..48461)
                     /gene="yhjD"
                     /locus_tag="RH222_000662"
     CDS             complement(47448..48461)
                     /gene="yhjD"
                     /locus_tag="RH222_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV3795725.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(48510..49409)
                     /locus_tag="RH222_000663"
     CDS             complement(48510..49409)
                     /locus_tag="RH222_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3795726.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            49929..50531
                     /locus_tag="RH222_000664"
     CDS             49929..50531
                     /locus_tag="RH222_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV3795727.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(50582..52231)
                     /locus_tag="RH222_000665"
     CDS             complement(50582..52231)
                     /locus_tag="RH222_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV3795728.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            52636..54033
                     /gene="ccp"
                     /locus_tag="RH222_000666"
     CDS             52636..54033
                     /gene="ccp"
                     /locus_tag="RH222_000666"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV3795729.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            54244..>55347
                     /locus_tag="RH222_000667"
     CDS             54244..>55347
                     /locus_tag="RH222_000667"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV3795730.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICT"
BASE COUNT        13211 a        13862 c        15007 g        13267 t
ORIGIN      
        1 aaaaaaatcc ccgccaaatg gcagggatct tagattctgt gcttttaagc agagattaca
       61 ggctggttac gttaccagct gccgggcctt tagcgccgct ttcgatggtg aaggacactt
      121 tctgaccttc gtccagagat ttgtaaccat cgttctggat agcagagaag tgtacgaaca
      181 cgtctttaga gccatcgtca ggagtgatga agccgaagcc tttgtcagcg ttgaaccatt
      241 ttacgatacc agtcatttta ccggacatag tgtattacct ttaataatta agtgtgcctt
      301 tcggcgatat ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca ctacgagggg
      361 tatcaacgat aactcttgaa gggacttgcc ttactacact gctttaatgg tctgtacgtc
      421 aaaccgttga tgtgcattaa gccacgcatt ggcgggtgat gcaacaatta tttttcatat
      481 ttatgattaa tcggttggaa aacggtcctg tcatcaggac cgtaaacagc aataaagtgg
      541 ataaaagtct attccatcaa ctgcttactt aatgccgggt tggcttgaat caaacgcatc
      601 aattttagtt cggcacttga aggcttcacg cgtctggatt cccattcctt taccatggcg
      661 actgatacgc ccaaaacccg ggcgaaatca tcgattttta atcctgtccc ttttcgtaac
      721 tgctcaattt cggtacagga cgttgttctg tgcgtcaggg taattttctg cgtttcatct
      781 ttaaaaacaa tctgttccag gctgctcaac agctcatgca ttggatcttt atattccatt
      841 gaaaactcct caaatcacac tgcgggatcg tgaattgcat cgaagctcat taagaatagt
      901 cgggaaaagc agacacagcg gggtgaagtc agggcatgct ctggctatga ttcgattcag
      961 ccagtcattt agcgtgactt atagccacct aacgatctga taaatgccga tatatctctt
     1021 accactcctg gtttgtaaag atttcgaaaa tctaaccttc tgcgaaggcg taaatctctg
     1081 gctacaaaaa aagcaacggc actatcattt ttttaacaat gaaacaacaa attaaccttg
     1141 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     1201 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     1261 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     1321 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     1381 gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac caaccctgac
     1441 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     1501 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggtcgtag acagcgttaa
     1561 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaacgg
     1621 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
     1681 ctctgcgcta aaacagaccg atgatttgaa accggtcttt gatcaggcat tcaccaaagt
     1741 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gctgcacaaa ctttcacgca
     1801 acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc
     1861 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     1921 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     1981 ataaagagta aaagcccgag cgattatcgg gctttacttc acgcagtcgc ggcttagtcc
     2041 gcgacgtgcg gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
     2101 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     2161 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggacagtgg ctcttgttcg
     2221 aagacatcca gcccggcggc gtgaatttcg cctttctgca atgcagcaat cagtgcattt
     2281 tcatcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     2341 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
     2401 aaatctgact cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     2461 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcgca
     2521 cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     2581 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     2641 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     2701 acggtttctg ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     2761 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
     2821 ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc agcttcggca
     2881 aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
     2941 aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     3001 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     3061 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     3121 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
     3181 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     3241 gatcaacgcg ctggcaacgg aatccgcacg ttgctgggag agacgcatgt tcaggtcgtg
     3301 accacccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     3361 caccattgcc acgccggtca gggtgttagc gcccgcaggt ttcagggtcg cgctgctgct
     3421 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     3481 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     3541 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
     3601 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
     3661 gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
     3721 cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca ttgttaatcc
     3781 ttaataacct tttttattct ttgccacggg ttccgtggcg ggagattatg ccgcgtgaac
     3841 atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca
     3901 tctaaccttt caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact
     3961 gccgcccact gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt
     4021 ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac
     4081 agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac
     4141 ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggat
     4201 cttattcacc aacaacataa acgcattcgt gaggcttatg gtccggcatc gatttttgct
     4261 ggttcctacg gctggcgttc aaacggcgtg ctgcataagg cctcgacatt attacaacgc
     4321 tatatggcgc tggcaggcgg ttataccggg catctggggg attattcgac tggtgcggca
     4381 caggcgatca tgccgtatgt cgtaggtggc agcgaagttt atcaacagca aaccagttgg
     4441 ccgctggtgc tggaacatag cgatgtcgtg gtgctgtgga gtgctaatcc actcaatacg
     4501 ctgaaaattg cgtggaatgc atccgatgag caggggcttt cttacttttc tgcactgcgt
     4561 gacagtggga aaaagctgat ctgcattgat ccaatgcgat cggaaaccgt cgatttcttt
     4621 ggcgataaaa tggagtgggt ggcaccgcac atgggcaccg atgttgcgct gatgctgggg
     4681 atcgcccata cgctggtgga aaatggttgg cacgacgaag cgtttctggc gcgttgcacc
     4741 acaggttatg ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat agcaaaaacc
     4801 gccgaatggg cagcagagat ttgtggtgtt ggcgcagcga aaatccgcga gctggcggct
     4861 attttccacc aaaataccac catgctgatg gcaggctggg gaatgcagcg ccaacagttt
     4921 ggtgagcaaa aacactggat gatcgtcacg ctggcagcaa tgttggggca aatcggcaca
     4981 cccggcggcg gttttggtct ttcttaccat tttgccaatg gtggtaaccc cacgcggcgt
     5041 tctgcggtgc tctcttccat gcagggcagc ttgccgggtg gctgcgatgc ggtggataaa
     5101 atccctgttg cccgcattgt tgaagcactg gaaaaccctg gtggcgcata tcaacacaac
     5161 ggtatgaacc gacatttccc ggatattcgt tttatctggt gggcgggcgg tgccaacttt
     5221 actcatcatc aggataccaa tcgtctgatc cgtgcctggc aaaaaccgga gctggtggtg
     5281 atctctgaat gcttctggac ggcggcggcg aaacacgcgg atatcgttct gcctgcgact
     5341 acctcttttg agcgtaatga tctcaccatg accggtgatt acagtaatca gcatctggtg
     5401 ccgatgaagc aagtggtgcc gccacgctat gaagcgcgta atgattttga tgtttttgcc
     5461 gagttgagtg aacgttggga gaagggcggc tatgcgcggt ttacggaagg aaaaagtcag
     5521 ctgcaatggc tggaaacgtt ttataacgtt gcccggcagc gcggggcaag ccagcaggtt
     5581 gaattgccgc catttgctga gttctggcaa gccaaccagt taattgagat gccggaaaac
     5641 ccggacagcg agcggtttat tcgcttcgct gatttttgcc gcgatccgct ggcgcatccg
     5701 ttaaaaaccg ccagcggcaa gattgaaatc ttctcacagc gtattgccga ttacggttac
     5761 ccggattgcc ctgggcatcc aatgtggctg gagccggacg aatggcaggg caatgccgaa
     5821 ccagaacagt tgcaggtact ttctgcccat ccggcgcacc gcctgcacag ccagctgaat
     5881 tacagttctc tgcgcgaatt gtacgcggta gcaaaccgtg agcctgtcac cattcatcct
     5941 gacgatgctc aggcgcgcgg cataaccgaa ggggatatgg tgcgggtgtg gaactcccgt
     6001 gggcagatcc ttgccggagc ggtcattagc gagggaatta aacctggcgt gatttgcatt
     6061 cacgaagggg catggccgga tctggattta accgctgacg gtatttgtaa aaacggcgca
     6121 gtgaacgtgc tgaccaaaga tctccccagc tcgcggctgg ggaatggctg tgcgggtaat
     6181 acggcgctgg catggctgga aaaatacaac ggtccggaac tgacacttac agcgtttgaa
     6241 ccaccggcca gctcataatc caggtgggta gttgggtttc atcctgccat gcgcaatcga
     6301 caatgtgaaa accctgcgca cggtaaaaat ctatcgccgg ttgatttttt tgataaacct
     6361 ccagcatcag atggggatag cgctgctgca catactgcat cagcgcctta ccaataccgc
     6421 gtctgacggc cttcggtgcg acaaacattg ctgccagaaa tcggccttcc ataatgctga
     6481 caaaaccgag aagcttaccg tcctcttccc agacccagtt ttgcgcgttg gcaagatagg
     6541 catcccgcac cagcggaatg cagtcacgcc agtaattcgc ttttataaag ggatgccccc
     6601 aggttgtact ttccagccac agttcgagga tcgcggggag ttctgaacgt tgcgcttccc
     6661 gaatcatggt ttatttcccg gatagcaaca gcagccaacc acatgatcat tcaccagccc
     6721 acatgcctgc ataaaggagt aacagattgt ggtgccgaca aacttaaaac cacgtttttt
     6781 cagtgcctta gatagggcgt cggaggcgga cgtagatgtg ggaatttcgc tcaacgttgt
     6841 ggcttgtgtc acctgtggct gatgatttac aaacgaccag acaaagtcgg caaacggttc
     6901 gccgttctgt tccatttgca ggtacgcccg cgcattacca ataattgcct gaattttccc
     6961 tcgatggcgg ataatcccgg cgtcctgtac cagtctttcg acatcctctt cctgcattgc
     7021 tgcgaccttc accggatcga actgatgaaa gcaggcgcga tagttttcgc gttttttgag
     7081 gacggtgatc cacgataacc cagcctgctg cccttcaagg cagatcattt cgaacagttt
     7141 tttactgtca gtttcaggca cgccccattc attatcatgg taggcaatat aaagcgggtc
     7201 ctgactcacc cagccgcaac gttccatact ttccctcgct atgtacccga tcgtaaaaat
     7261 ttcagccgct tttcttgacg catcggtgaa aaagtcaata tttaatacag ggctactatg
     7321 cccgatatcc ttcatgcaat gacaataata ttgccgaatt cggctcttcc ctggagtcgt
     7381 gttaatgatc ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg cgagcgtggt
     7441 aatcagtgcc ttttttctca acacagctta cgcctggcaa caagaatata tcgttgatac
     7501 gcaacccgga cattccacag agcgttacac ctgggatagt gatcatcaac ctgattacaa
     7561 cgatattttg tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct
     7621 ggcggaagaa actcctgtgg atgtgaccag cagtatgagt atgggctgga attttccttt
     7681 atatgaacag gttacaaccg gcccggtcgc ggcattacat tacgatggca caaccacctc
     7741 gatgtataac gagtttggcg acagtactac cacgctgacc gatccgttat ggcatgccag
     7801 cgtgagtagc ttaggctggc gtgttgactc ccggcttggc gatctccgac cctgggcgca
     7861 aatcagctat aaccagcaat ttggcgagaa tatctggaag gcgcaatcag gcctgagccg
     7921 gatgacggcg acaaaccaga acggcaactg gctggatgtc accgtaggcg ctgatatgtt
     7981 gctcaatcaa aatattgccg cctatgccgc gctaactcag gcagaaaata ccactaataa
     8041 tagcgactat ctgtatacga tgggggttag cgccagattt taacgtaaca gtcacaattg
     8101 aaaccattga ataacaacag ttgtgtcgat agtgggtgct aacttaccaa ataataaatt
     8161 tggtgaataa ttgtcgcgtc attcattcct gaactaaggc atttcattcc gttctgatgg
     8221 catttcatgc cgtttttccc caggcataaa atgcacttcg ttatggttgt cggcagagat
     8281 ttttcctttt tattactgca ggaatactgc catgacacct tcaaattatc agcgtacccg
     8341 ctggctgaca ctcatcggta ctatcattac ccagtttgca ctggggtcgg tttatacctg
     8401 gagcctgttt aatggcgcgc tttctgccaa gctggatgcg ccggtaagcc aggtcgcttt
     8461 ctctttcggc ttgttaagtc tggggctggc aatttcgtct tctgttgcgg gcaaattgca
     8521 ggaacgtttt ggcgttaaac gcgtcaccat ggcttccggc attttgctgg gattaggctt
     8581 cttcctgacc gcgcattcca acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt
     8641 cggtctggcg gatggcgcgg gttacctgct gacgctctct aactgcgtga agtggttccc
     8701 ggagcgtaaa ggtctgatat ccgcgttcgc tatcggttct tatggtctgg gtagcctggg
     8761 tttcaaattt atcgacacgc agctgctgga aacggtgggt ctggaaaaaa cctttgtgat
     8821 ttggggagcg attgcgctgg tgatgattgt tttcggcgca acgttaatga aagacgcacc
     8881 aaaacaggaa gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc tggcagagtc
     8941 gatgcgtaaa ccgcagtact ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg
     9001 cctgtacgtg attggggtag cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt
     9061 ttccgcagcc aatgcagtaa cggttatttc catcgctaac ctttcaggtc gtctggtgct
     9121 gggcattctg tctgacaaaa ttgcccgtat ccgtgttatc accattggtc aggtgatttc
     9181 gctggtgggt atggcggccc tgctgtttgc accattaaat gcagtgacgt tctttgcagc
     9241 gattgcctgc gtggcattta actttggcgg cactattacc gtctttccat cactggtcag
     9301 tgagttcttc ggcctcaata acctggcgaa aaactacggt gtgatttacc tcggtttcgg
     9361 tatcggtagc atttttggtt cgattatcgc ctcactgttt ggcggtttct atgtgacctt
     9421 ctacgtgatt ttcgccctgc tgattctgtc attggcgctt tctacgacga ttcgtcagcc
     9481 agagcagaaa atgttgcgtg aggcgcatgg ctccctttaa tgctcagccg cgagctactg
     9541 ggcttcagct tccgtgtgca tcaaacgcat caacgaaatg tataaacaat gactcgtaaa
     9601 atttgggcca gttaactggc cctttttact gctttttgta acgctaaagt ctcttttcaa
     9661 acttgcattt ttgtaaattt gtgcttcatg cacactcttt ccccacactt tttcgctttg
     9721 ctgtggtcta cttattcgcg cgtgtagatt ttacttatct gactacctcc gcacttttcc
     9781 cctgccgggc ctgaaaagcc gctaagcagg gtgttatcac ctgtttgtcc agggtttgtt
     9841 tgcatgagat acatcaaatc gattacacag cagaagctga gctttttgct tgcaatctat
     9901 attggccttt ttatgaatgg cgcggttttt tatcgccgct tcggcagcta tgcgcacgat
     9961 tttaccgtct ggaaaggcgt ttctgctgtt gttgaactgg ccgccaccgt actggtgacc
    10021 ttctttttac tacgtcttct atcgctgttt ggccgccgca gctggcgtat tctggcgtcg
    10081 ctggtggtgc tcttttccgc aggtgccagc tattacatga ccttccttaa tgtggtcatt
    10141 ggttatggca tcatcgcttc cgtcatgacc accgatatcg acctgtcaaa agaagttgtt
    10201 ggtctgaact ttattctctg gttaatcgcc gttagtgcat tgcctcttat ccttatctgg
    10261 aataaccgct gtcgctacac cttgctccga caactgcgaa ccccagggca gcgtattcgc
    10321 agcctggcgg tcgtcgtact ggcgggtatt atggtttggg caccgattcg tttgctggat
    10381 atccagcaga agaaagtgga gagggcgacc ggcgttgatt tgccgagtta tggcggtgtc
    10441 gtagcgaact cttatctgcc atcaaactgg ctttctgcgt tggggctgta tgcctgggcg
    10501 cgggtcgatg aatcttccga taataattca ttgcttaatc cggcgaagaa attcacttat
    10561 caggcaccgc aaaacgttga tgacacttat gtcgtgttta tcatcggtga aaccacgcgt
    10621 tgggaccata tgggcatttt cggctatgag cgtaatacca cgccgaaact ggcccaggag
    10681 aaaaatctgg cggcgttccg tggttactcc tgtgataccg caaccaaact ctcactgcgt
    10741 tgcatgtttg tacgtcaggg gggcgcggaa gataatccgc agcgcacatt aaaagaacag
    10801 aacattttcg cggttctgaa gcagttagga ttcagttctg acctctacgc tatgcagagc
    10861 gaaatgtggt tctacagcaa cacgatggcg gacaacattg cttatcgtga gcagattggt
    10921 gcggagccac gtaatcgtgg caagccggta gatgatatgt tgctggtaga cgaaatgcag
    10981 caatcgctag ggcgcaaccc ggatggtaag catctgatca ttctgcatac caaaggttcg
    11041 cattttaact acacccagcg ttatccgcgt agcttcgcgc agtggaagcc ggaatgtatt
    11101 ggtgttgata gcggctgtac caaagcgcag atgatcaact cctatgacaa ctcggtgacc
    11161 tatgtggatc actttatctc cagcgtgatt gatcaggttc gcgataagaa agcgattgtg
    11221 ttctacgcag ctgaccacgg tgagtcaatt aatgaacgcg agcacctgca cggcacgccg
    11281 cgtgaactgg caccgccgga gcagttccgc gtaccgatga tggtctggat gtcagataaa
    11341 tatctggaaa atccggccaa tgctcaggca tttgcgcagc tgaaaaaaga agccgacatg
    11401 aaagtgccac gccgtcatgt agagctgtac gacaccatca tgggttgtct tggctatact
    11461 tcaccggatg gtggaattaa cgaaaacaac aactggtgtc acatcccgca gacaaaagtg
    11521 gcagcggcta actaagcggc ctgctgactt tctcgccgat caaaaggcat tttgctatta
    11581 agggattgac gagggcgtat ctgcgcagta agatgcgccc cgcattcggt gattggcgca
    11641 gcctggtagc gcacttcgtt cgggacgaag gggtcggagg ttcgaatcct ctatcaccga
    11701 ccaaattcga aaagcctgct cagcgagcag gcttttttgc atttgaagcc catgaggatg
    11761 agaacctccg ggggcgggga ggttcgactc gagcgaagcg agagaacgtt gcgccagcaa
    11821 cggcccgcag ggcgagccac gaagtggcga gtaatcctct atcaccgacc aaattcgaaa
    11881 agcctgctca gcgatcaggc tttttttgca tttgtagccc atgaggatga gaacctccgg
    11941 gggcagggag gttcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa
    12001 cggcaccgca cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca
    12061 ttttcctgcc aactttgcgc cccatatctc actatccatt aacggatttg tgacagaaac
    12121 cattgtcttt ttttatattt gcttaaatct ttttttgcgt tacttatggc tggcgctggc
    12181 attttccgct gtgcgtttta gcgttcatgg tattaatcgc tcagatatcc accttttcct
    12241 cagatttttt tcattaaatg cataaaactt aatcaccaaa tgttgctaaa tattaagctg
    12301 attgttctgg tcatgttgga gtagcacaaa tttctctgct gcaaatagct gtttgaagtt
    12361 tttttaatct ttgtttgtgg attctcaccg ttggtgtaaa tcccggttgg ctgtattgac
    12421 gttttcacat tctgttgaca gattgtaggt cacgaggggc attttatgga ggatccgcac
    12481 tgttacactg atgttaatta gtacggcatc cccacctcat aacgttgacc cgaccgggca
    12541 aaaaacaaaa aaggtcaggc agcgacaacc cactgcaaag ggttaaaaca acaaacatca
    12601 caattggagc agaataatgc gtatttcctt gaaaaagtca gggatgctga agcttggtct
    12661 cagcctggtg gctatgaccg tcgcagcaag tgttcaggct aaaactctgg tttattgctc
    12721 agaaggatct ccggaagggt ttaacccgca gctgtttacc tccggcacca cctatgacgc
    12781 ctcttccgta ccgctttata accgcctggt tgaatttaaa atcggcacca ctgaagtgat
    12841 cccgggcctc gctgaaaagt gggaagtcag cgaagacggt aaaacctata ccttccatct
    12901 gcgtaaaggt gtgaagtggc atgacaataa agaattcaaa ccgacgcgcg aactgaacgc
    12961 cgatgacgtg gtgttctcgt tcgatcgtca gaaaaacgcg caaaacccgt accataaagt
    13021 ttctggcggt agctacgaat acttcgaagg catgggcttg ccggagctga tcagcgaagt
    13081 gaaaaaggtg gacgacaaca cagttcagtt cgtgctgact cgcccggaag cgccgttcct
    13141 cgctgatctg gcaatggact tcgcctctat tctgtcaaaa gaatatgctg atgcgatgat
    13201 gaaagccggt acaccggaaa aactggacct caacccaatc ggaaccggtc cattccagtt
    13261 acagcagtac caaaaagatt cccgtatccg ctacaaagcg tttgatggct actggggcac
    13321 caaaccgcag atcgatacgc tggtcttctc tattacccct gatgcttccg tgcgttacgc
    13381 gaaattgcag aagaatgaat gccaggtgat gccgtacccg aacccggcag atatcgctcg
    13441 catgaagcag gataaatcca tcaacctgat ggaaatgccg gggctgaacg tcggctacct
    13501 ctcgtataac gtgcagaaaa aaccgctgga tgacgtgaaa gttcgccagg cactgaccta
    13561 cgcggtgaac aaagacgcga tcatcaaagc ggtttatcag ggcgcgggtg tatcagcgaa
    13621 aaacctgatc ccgccaacca tgtggggcta taacgacgac gttcaggatt acacctacga
    13681 tcctgaaaaa gcgaaagcct tgctgaaaga agcgggtctg gaaaaaggtt tctccatcga
    13741 cctgtgggcg atgccggtac aacgtccgta taacccgaac gctcgccgca tggcggagat
    13801 gattcaggca gactgggcga aagtgggcgt gcaggccaaa atcgtcacct acgaatgggg
    13861 tgagtacctc aagcgtgcga aagatggcga acatcagacg gtaatgatgg gctggactgg
    13921 cgataacggg gacccggata acttcttcgc caccctgttc agctgcgccg cctctgaaca
    13981 aggttctaac tactcaaaat ggtgctacaa accgtttgaa gatctgattc aaccggcgcg
    14041 tgctaccgac gaccataaca aacgcgttga actgtacaag caagcgcagg tggtgatgca
    14101 cgatcaggct ccggcactga tcatcgctca ctccaccgtg tttgaaccgg tacgcaaaga
    14161 agtcaaaggc tatgtggttg atccattagg caaacatcac ttcgaaaacg tctctatcga
    14221 ataattaaaa gccatacaag actgatggca aaggcaaaac tgcctgatgt gctccgctta
    14281 tcaggcctac gaaaattctg caatgtattg aatttgcacg attttgtagg ccggataagg
    14341 cgttaacgcc gcatccggca taaacaaagc gcactttgtc aacaatctgt atacccggtg
    14401 gcgctgtgcc tctgcgtgca gcgccatccg gcagcaatac ttcattctct gtccgacaag
    14461 ggcgggaatt gatttgtgag caatacagac acgcagttcc aggctgcggg tcactataga
    14521 gaatccgggt tatgttgcag tttattctcc gacgtttggg actcgtcatc cccacgttta
    14581 tcggtattac ccttctcaca tttgcctttg tccacatgat cccgggcgat ccggtgatga
    14641 tcatggcagg cgaacgtggg atctcccctg agcgtcacgc gcagctgctg gctgaactcg
    14701 gcttagataa accgatgtgg cagcagtatc tccattacat ttggggcgta atgcacggcg
    14761 atctaggcat ttcaatgaaa agccgaattc cagtatggga agagttcgtg ccgcgctttc
    14821 aggccacgct ggaacttggc gtctgcgcga tgatttttgc tacggcagtc ggtattccgg
    14881 ttggtgtgct ggctgcggtt aaacgcggtt ccattttcga tcacacagcg gttggcctgg
    14941 cgctgaccgg ctactcgatg ccgatcttct ggtggggcat gatgctgatc atgctggttt
    15001 cggtgcactg gaacctgacg cccgtctccg gtcgcgtgag cgatatggtg ttcctcgatg
    15061 actccaatcc gttaaccggt tttatgctaa tcgacaccgc catctggggt gaagacggca
    15121 actttatcga tgccgtcgcc catatgatct tgcctgccat tgtgctgggt actattccgc
    15181 tggcggtcat tgtgcgtatg acacgctcct cgatgctgga agtgctgggc gaggattaca
    15241 tccgcaccgc gcgcgccaaa gggctaaccc gcatgcgggt gattatcgtc catgcgctgc
    15301 gtaacgcgat gctgccggtg gtgaccgtta tcggcctgca ggtgggaaca ttgctggcgg
    15361 gggcgattct gaccgaaacc atcttctcgt ggcccggtct gggacgctgg ttgattgacg
    15421 cactgcaacg ccgcgactat ccggtagtgc agggcggcgt attgctggtg gcgacgatga
    15481 ttatcctcgt caacttgctg gtcgatctgc tgtacggcgt ggtgaacccg cgtattcgtc
    15541 ataagaagta aggggacatc atgtcacagg ttactgaaaa taaagtgatt agcgcaccgg
    15601 tgccgatgac cccgttacag gagttctggc actattttaa acgcaacaaa ggcgcggtcg
    15661 tcgggctggt ttacgtcgtc atcgtgctgt tcatcgcgat ctttgccaac tggattgcac
    15721 cctataaccc ggcggaacag ttccgcgatg cactgctcgc cccgccagcc tggcaggaag
    15781 gcggcagcat ggcgcacttg ctgggcaccg atgacgtagg ccgtgatgtg ctgtcgcgcc
    15841 tgatgtacgg tgcgcgcctg tcgctgctgg ttggctgtct ggtagttgtg ttatcgctga
    15901 ttatgggcgt tattctcggc ctgatcgccg gttactttgg cggcctggtc gataacatca
    15961 ttatgcgcgt ggtcgatatc atgctggcgc tgccaagtct gctgctggcg ctggtgctgg
    16021 tggcaatttt cggcccgtcg attggtaacg ccgcgctggc actgaccttc gttgccttgc
    16081 cgcactatgt gcgcttaacc cgcgccgccg tgctggtgga agttaaccgc gattacgtca
    16141 ccgcgtctcg cgtggcgggt gccggggcga tgcgtcagat gtttattaac atcttcccga
    16201 actgccttgc gccgctgatt gttcaggcgt cgctcggttt ctctaacgcc attctcgata
    16261 tggctgctct tggtttcctc ggcatggggg cacagccgcc aacgcctgag tggggcacca
    16321 tgctctccga cgtgttgcag ttcgcgcaaa gcgcctggtg ggtcgtgacc ttcccgggtc
    16381 tggcgatcct gctgacggtg ctggcattta acctgatggg tgacggtctg cgtgacgcgc
    16441 tcgatcccaa actgaagcag taagaggttc gagatggcgt tattaaatgt agataaatta
    16501 tcggtgcatt tcggcgacga aagcgcgccg ttccgcgccg tagaccgcat cagctacagc
    16561 gtaaaacagg gcgaagtggt cgggattgtg ggtgagtccg gctccggtaa gtcggtcagt
    16621 tcactggcga ttatggggct gattgattat ccgggccgcg taatggcaga aaaactggag
    16681 tttaacggcc aggatttgca gcgtatctca gaaaaagagc gccgcaacct ggtgggtgcc
    16741 gaagtggcga tgatcttcca ggacccgatg accagcctta acccgtgcta caccgtgggt
    16801 ttccagatca tggaagcgat taaggtgcat cagggcggta ataagagcac tcgccgtcag
    16861 cgggcgattg acctgctgaa tcaggtcggt attcccgatc cggcatcgcg tctggatgtt
    16921 tacccgcatc agctttccgg cggcatgagc cagcgcgtga tgatcgccat ggcgattgcc
    16981 tgtcggccaa aactgctgat tgccgatgaa ccgaccaccg cgctggacgt gaccattcag
    17041 gcgcaaatca tcgaactact gctggagcta cagcagaaag agaacatggc gctggtgtta
    17101 attacccatg acctggcgct ggtggcggaa gcggcacata aaatcatcgt gatgtatgca
    17161 ggccaggtgg tggaaaccgg tgatgcgcac gccatcttcc atgcgccgcg tcacccgtat
    17221 actcaggcat tgctgcgtgc gctgccagaa tttgctcagg acaaagaacg tctggcgtcg
    17281 ttgccaggtg tcgttcccgg taagtacgac cgcccgaacg gctgcctgct taacccgcgc
    17341 tgcccctatg ccactgacag atgtcgcgct gaagaaccgg cgctgaatat gctcgctgat
    17401 gggcgtcagt ccaaatgcca ttacccactt gatgatgccg ggaggccgac actatgagta
    17461 cgcaagaggc cacctcgcaa caaccgctgt tgcaggctat cgacctgaaa aaacattatc
    17521 cggtgaagaa aggcatgttc gcgccggaac gtctggtgaa ggcgctggac ggcgtttcgt
    17581 ttaaccttga acgtggcaaa acgctggcag tagtgggcga atctggctgc ggtaaatcga
    17641 ccctcggtcg gttgctgacg atgatcgaaa cgcccaccgg tggtgagctg tattaccagg
    17701 ggcaggatct gctcaagcac gatccgcagg cgcagaagct gcgtcggcag aaaatccaga
    17761 ttgtcttcca gaacccttac ggttcgctga atccgcgtaa aaaagttggg caaattctcg
    17821 aagagccgct gcttattaac accagcttca gcaaagaaca gcgtcgggaa aaagccctgt
    17881 cgatgatggc gaaagtcggc ctgaaaaccg agcactatga ccgctatccg catatgttct
    17941 ccggcggaca acgtcagcgt atcgctattg cccgtggtct gatgctcgac ccggacgtgg
    18001 tgattgccga tgaaccggtt tctgcgctgg acgtctcagt gcgtgcgcag gtgctgaatc
    18061 tgatgatgga tttgcagcag gagttggggc tttcttatgt ctttatctcc cacgacctgt
    18121 cggttgtgga gcacattgct gatgaagtga tggtgatgta cctgggccgc tgcgtggaga
    18181 aaggaacgaa ggaccaaatc ttcaataacc cgcgccatcc gtacacccag gcgctgctct
    18241 ccgcgacgcc acgcctgaac ccggacgatc gtcgtgaacg catcaaactc accggtgaac
    18301 tgccaagccc gctgaatcct ccgccgggtt gcgccttcaa cgcccgctgt cgtcggcgct
    18361 tcggcccctg cacccagttg cagccgcagc taaaagacta cggcggtcaa ctggtagctt
    18421 gttttgctgt tgatcaggat gaaaatccgc agcgttaact cacaaaaccg gggccatgcg
    18481 ctccggttta ctcaatcagc ttcaacagtg gcgacaagca tagcaaaatg ccgtaaagca
    18541 gaattagcca ggctttaaat ccgcgaagtt tttccagttg tgcgacttta tagatcagga
    18601 aaaacggaat gaggcacgac acaatgccat ataacgggct tcccaactga aagaacacca
    18661 gcaccgatac acgaaacgaa acccaaatcg tcaatgtaat gacgataaaa gcgcagatcg
    18721 ccagagtcag catgcgtgag ttaattttct tggtatcaat aattcggctt aacagattga
    18781 gaataatgcc tttaatagcc tcgtggaaac cgagataaat gccaaagaat gcggtcagta
    18841 cggcaaagat attcagcacc gtagaggtga tatgaatgat atgtccaggg atcacctgcg
    18901 cggccagcgc cagtgccgag atattttgtt caaaggcaga aacggcttct tcgtggctaa
    18961 ttgagaaggt aaacgaaaag gcaaaaaaca ggatcaccgc gatgagcgtg atataactaa
    19021 ttcggtgggt acgcagcgcg agccgggttg ccagtacttt atctgcttcc cgtttacgat
    19081 aggcaatatt cattgggtta agtacctgaa taaataccgc agaaaagaag caaaatggaa
    19141 ttgtaagcag gacatcgcgg aaaaagacgg aggcttgcgg gaaggcggtt atattggcga
    19201 aattccagtg cgggatcatc gcaaaaccga acacgacaat aatccctact ttgaccacca
    19261 ccattggccc ggaaatctta aacagtaatc gttcaccacc agacgcaatc gccaccagta
    19321 cggcgaaaat agcgacttta taaagtagag attgtgaaag atcggcatcg gttaaaccga
    19381 aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag agagtagata aaaatcccgt
    19441 ggataatcat caaaaagtag ataaccccga ggaaaattcc ccagttcttc cccaggtaat
    19501 gactgataat atcggtgtag tcattacagg aatcgctttc agaaagggtt tttaaataaa
    19561 tgtcctgcac tacccaggtg gcaggataag caatgatcgc tgcggtaata aatacccaaa
    19621 ttcccttcaa gccaatttgt actggcatca gcacggttcc ggcaccaatc gccatgccta
    19681 tgcataataa aacccagccg aagtcgtagc gtgtaaacgg caatttctga ttgtgtttcg
    19741 atagtgtgtt gtgctgcata aagtcctgtc cagaaagaag tcggcctcgc aaaaatgaat
    19801 aaacccagtt tctcttgcgc aacaggtgga gaagtcgaat acgcagtatg taaaaaatga
    19861 ggcaacagat tcactgggga ggtcgcgagg aggcgcaatt ctacaaaatt gcgtatttca
    19921 ctaccagtct ggagtcagcc cggatatgaa ctgcaactat tgcaaataaa gaaattaagc
    19981 tggaaggcat cgcgcaatga ggaagattgc cgggtgctga cagtaaaaag caatcgtaaa
    20041 atcagcggca agccggaagg gttccggtga ggcgcaatgt tgcgggggct ttatccctgg
    20101 tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatatac ctgacaacat
    20161 aacgggggct aatcttgaac ctagaccact caagaatagc cgcgaaacgt tgtcattaca
    20221 acacaggcgg ctatatgacg ctcgcagagc tgggcatggc cttctggcat gatttagcgg
    20281 ctccggtcat tgctggcatt cttgccagta tgatcgtgaa ctggctgaac aagcggaagt
    20341 agcgtgtcat gtgggcgtca ggctgccgta atggcaattt gcgcccggac caggccgcag
    20401 gagtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt
    20461 ctgcggaatg cgcagcacag agtgaagatg agacggattc cggaacagaa aaacagtcgt
    20521 agaatcagcg tcaagccgga aggattccgg tgaggcgcaa tgttgcgggg gcttaatccc
    20581 tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa
    20641 caccacgggg gccattcttg catccaaaca atacaagaat agccgcgaaa cgttgtcatt
    20701 acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag
    20761 cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg aacaagcgga
    20821 agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg gatcaggccg
    20881 caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca agcggataat
    20941 attctgcgga atgcgcagca cagagtgagt aagtgatgga ttccggaaca gaaaaacagt
    21001 cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg ggggctttat
    21061 ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt atgcacctga
    21121 caacatcacg ggggccattc ttgaacctga acaactcaag aatagccgcg aaacgttgtc
    21181 attacaacac aggcggctat atgacgctcg cagaactggg catggccttc tggcatgatt
    21241 tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg ctgaacaagc
    21301 ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc ccggaccagg
    21361 ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt gcaagcggat
    21421 aatattctgc ggaatgcgca gcacagagtg agtaagtgat ggatttcgga acagaaaaac
    21481 agtcgtagaa tcagcggcaa gccggaaagg ttccggtaag gcacaatgtt gcgggggctt
    21541 tatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgcacc
    21601 tgacaacata acgggggcta atcttgaacc caaacaactc aagaatagcc gcgaaacgtt
    21661 gtcattacaa cacaggcggc tatatgacgc tcgcagagct gggcatggcc ttctggcatg
    21721 atttagcggc tccggtcatt gctggcattc ttgccagtat gatcgtgaac tggctgaata
    21781 agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc
    21841 aggccgcaga ggttaaactc tgcggccttt ttcgtgctta ctgcgggtaa ggcacccagt
    21901 cgccgccgtt caggcgaacg tacggtttat cctggtattg aataactact gcatttgagt
    21961 tctcggagac cggtgctgtt tgtggcaacc cactggtgag ttttttccag tcaacattgt
    22021 cttcggtgaa aatcttgcca tcaagaacgc gaaccaccag atcggagata gccaggaagc
    22081 tgctcggttg gtcaatgaca atcggtgccc cctgatgtgg tgccttcatg ccgaagaatt
    22141 tcaccccaac ggggacgtct gtgatagacg ggctagggat atcacgtagg ccagatacct
    22201 gcattctgtc gcccttcagc gcgccgccgt gttccggcac cacgaccacc atcactttac
    22261 gacccgattt ctccagttca gtaaagaagg cgtccagttc atcaaagaat ttctgcgccc
    22321 gcgctttgta atccgctgtt ttgctgaccc ccggataatg gttgccgtca tgcagtggaa
    22381 gcgtgttgta gaacgtggca ctacggctgt ttttatcttt ttcggtaacg tccagccagc
    22441 ggttaagcac ggcggtatcg tcataaaccg gcgaaccatc aaagcccagc aaaataaccg
    22501 gcagatttgt ttgatccatc aattcagtct gcatgccgcc attttcgcga acttctttca
    22561 aaaaaccgcc gaactggccg ttatgcccca tcatcaggtg ctgggtaaag cccagtttcg
    22621 aaaggttatc aaacagatag cagtcgttat ttgccggttg atacagatta gtgtgcgaag
    22681 tctgcccgca gctggcgcgc agtaaacgga tcgccgccgg gccactgtag gaggtggcgg
    22741 agttaaagtt cttgaactca atatcgaaat gcgaccacag tggatgcgac atcaacccgg
    22801 cggcttctat atccgaccag gaaagcgaac agatgttaat caccagtagt tcaaatggct
    22861 gagcatcagc gggcagcgaa gacgggaagg tcgatttacg ttttgcctcc gcgttataga
    22921 aattattcag ccaggcgtta aggttcgccg ttgttggcgg tgcagtttgt gcgggcatat
    22981 cacccactac cggtgcgcca cccgtcgccg caacggttgc cgctgcgtta ccacccgtcg
    23041 ttgttacagt ggtcgtcggt tgtccggctg gccacaagga gaaacttggt cccgccaggg
    23101 taagtacgtt cagccatagc agtatggcaa ccacaaaaac ggtaatgcga atccattgtg
    23161 acaggaataa ccaggccact aataaaacaa aaatggcccc aatcatctgc cagttaataa
    23221 agcgtgtgac aaggtcgatt aaataatcgg tactgaaccc cgccacctgc gaaccctggc
    23281 tcattatgct ttccgggcca ggcaaccagg tgtcatgcca gaacaaagca aagccgatcg
    23341 gcagggcaat ccagtggcgc aagcgatgca ggctgtagcg cggaatgggc atcagcagaa
    23401 acgcggcaaa caccaaattg aggagcggat ggaagttaag atatcccgcc cacaacaggc
    23461 cgaacttaac cagaaaatag aagttccagc cggaaaggcc gcgccagtat tgccagaggg
    23521 aagaaggcat ggcggtattt tgcgtaaatt gagtcatttt ttggttgccc tggctttttc
    23581 cgtgcggcgt aacgtcccgg ccggtttaac ataacgaggt ttagcaaaga acaaacgtaa
    23641 ggtgtcgcga atgcgtcgca aagagtgccg cgccagatag cccagcggga aaaatatcag
    23701 tgcgcaaacg acaataattt caatgatatc gctgatggtc atcatgatga gcgctccaca
    23761 gcatcatcta acagtcgcat gggttctggt attcgtcgcc agtggcgacc atcgtgctcg
    23821 gcattgatga ccggtttaga actttgcgtt aaaggcagcg gcatgcccca ttgttctggg
    23881 gcaagcaagc gcatctgcac cagctcggca ctgatttgat catcttcaaa ccagaccata
    23941 cggtttgaga aaatgtcgcc agtaggcaat gggaaaatat gattcaacgc ggtatccaga
    24001 tcgttaatcc gacagaatga gagaaacagc accagccgat taccgccaat ggtcatgata
    24061 tcgccggtac ggttagggcg acacagcgtc agggcttgtt caacgcggat acccggtacc
    24121 ggacgtaggg caaccagaac gcctttaccg tgggcaggta atagagggtt attcatcatg
    24181 ttgttgacgg cattacagaa cacatcccac ttctggaaac cacgcagttt gagcggctgg
    24241 gtcattgaca gcaaggtagt gatatcttcc ggcacatagc gactaaactt ctgcccttgc
    24301 acgctttcga tcatcgtcag acaacgggag agtggcgcat tccacggaat aaccatattt
    24361 gcaccgcagg ccaataacaa acgttcatcg gtggcgcgca ggctagcggt attttcccgc
    24421 acgaggattt tcatcgcact accgcgctgg cgacgcaggg tatgaatgct gcgggccagt
    24481 ggctcgattt gcgcattttg ctgtaaagaa aagaccaccg tcgccgcctg agcggtacgg
    24541 gcttcattga acaggacttc gttattgttg aacagttgcc agtgttccga tagcggcggc
    24601 gcaccttcca gtacagcaac attactcaga atgcgttttt cgtcgctgcg tggttggatc
    24661 tccgcctctt cgctttgaac taatgtccag ataccatttt gttgctgaac gctaagctgc
    24721 tgacgggcgc tgaccccttt ttcgttgcac cagaaggcaa tatccagcaa atgttggtca
    24781 ccctgaaaac gcaaactggc aagaccaaaa agtgaacggt attcctcaag caacaatgaa
    24841 aattgtttat cgttattatt tccgggatta attaccaaaa gcgaacaatg gtttaacctg
    24901 ctccatttat tcattttatc caaccatgag cgaagccgct cggcaggaat gttttgccat
    24961 gcgttatttg cgcaaacaag aataaaaagg taattatggg gatcaataga acactgcaaa
    25021 tcacgggtca aatagtatag acctttttca tgatttagca ttgaaaataa ttttattttt
    25081 tccggccctt gagaatcatc taattgaaag attttcgccg gatcgctgtc catgctaatg
    25141 accgcgacgt gtgcggtttc agcctgggat gcaattgttt gattcgccag actgatagca
    25201 tcttcatggc gatcgacgtt aaaccaccag acgccgcctg ctggcatatg tcgcagctca
    25261 tcccataatg atgagatacc gatagagaat acagggtcca caatgtccct catgtcacca
    25321 ttttgtctga ttatcagttt actagcgaaa gctaagaaat aaacctacca ttgaaattaa
    25381 aacttatcag atttagcatg tgaatcaatt tagaaggtca caagacgtgt gattttagtc
    25441 ttttcgtttt taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt
    25501 ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag aattgtctca tgaacggtac
    25561 ggttatttca tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc
    25621 cgcgataggc tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc
    25681 tgatattgat tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg
    25741 gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga
    25801 ttgcaggggg tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca
    25861 ttacaaatgt tgggagaaaa tgttctggtg gtcgatgcct gcccggacaa cttgttgcgc
    25921 ctgtcattta atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc
    25981 caggactggc gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt
    26041 cagttatcca ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt
    26101 tgctccggct tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta
    26161 ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggct
    26221 atcgtcaatg tggatgccaa ctgccatatc cgcctgcatc agcaagcgct gccggatggc
    26281 gcacatattt tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag
    26341 ctttggttgc aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg
    26401 gctgaatgcc tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct
    26461 gaagagatac tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca
    26521 gtcgggagtg catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc
    26581 ggcttatcgg gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc
    26641 tcggctgttt ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctggc
    26701 agcgtattcg cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc
    26761 cgctggaccc ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca
    26821 aagaaacgcc gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt
    26881 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg
    26941 agaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
    27001 tcaccttctc gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc
    27061 agtttatctt cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc
    27121 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
    27181 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
    27241 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
    27301 ggccgctgaa tcgtcagccg gtgccattgc cgaaagatat gtcgctgtgg ccgtcggtgg
    27361 atatctttgt cccgacttac aacgaagatc tcaacgtggt gaaaaatact atttacgcct
    27421 cgctgggtat cgactggccg aaagacaagc tgaacatctg gatcctcgat gacggcggta
    27481 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgtaccactc
    27541 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
    27601 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
    27661 ggttcctgaa agagaaacag ctggcgatga tgcagacgcc gcaccacttc ttctcgccgg
    27721 acccgtttga acgcaacctg gggcgttttc gtaaaacgcc gaacgaaggc acgctgttct
    27781 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
    27841 cagtaattcg tcgcaaacca ttggatgaaa ttggcggtat tgctgtcgaa acggtaaccg
    27901 aagatgcgca cacgtctctg cgcctgcacc gtcgtggcta cacctctgca tatatgcgta
    27961 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
    28021 gctgggcacg cgggatggtg caaatcttcc gtctcgataa cccgcttacc ggtaaagggc
    28081 tgaaattcgc ccagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
    28141 cacgactgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
    28201 atgcgccagc gttgatgatc gccctgttcg tgctgccgca tatgatccat gccagcctga
    28261 ccaactccaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
    28321 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
    28381 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
    28441 cgcggcccta catcttcctt gtcctgctca acctggtggg cgttgcggta ggcatctggc
    28501 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtat
    28561 tctacaacct gattgttctt ggcggcgcag ttgcagtatc ggtagaaagc aaacaggtac
    28621 gccgatcgca ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
    28681 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
    28741 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
    28801 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
    28861 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
    28921 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
    28981 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
    29041 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
    29101 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
    29161 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
    29221 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
    29281 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct
    29341 gatgcgccag tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
    29401 attgcaccgc cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag
    29461 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca
    29521 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
    29581 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
    29641 aacccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
    29701 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
    29761 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
    29821 ttctttgacc cgcgcgataa ccgtactaac accttgccaa tggtctttgc gggtgcgccg
    29881 gatgttgggc tgcaacaagc ctctgccatt gtcgcctcgt ggtttggttc gcgttctggc
    29941 tggcgtgggc agaacttccc ggtgctctat aaccaactgc cggatcgtaa tgccattgtc
    30001 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagcccct
    30061 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
    30121 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
    30181 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
    30241 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
    30301 gaacagttac aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg
    30361 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
    30421 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
    30481 tccttcaacc tgagcagcaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta
    30541 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
    30601 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
    30661 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
    30721 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
    30781 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
    30841 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
    30901 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
    30961 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
    31021 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
    31081 attgtgccgg acgagagcga tcgcgcggca gaaacccggt caacgctgac ctcttccggt
    31141 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
    31201 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
    31261 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
    31321 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
    31381 ctggcaaacc atccgattct gctggcggtg ctggcggcta tcagtgtgat attgctggca
    31441 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
    31501 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt
    31561 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
    31621 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
    31681 agttacggta tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc
    31741 gactggacgc agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg
    31801 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    31861 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    31921 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    31981 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    32041 tttaacccca gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    32101 tggaccacgc tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt
    32161 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    32221 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    32281 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    32341 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    32401 gtcggttttt ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag
    32461 cgccagcgcg tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc
    32521 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    32581 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    32641 tggccgtcat gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag
    32701 ttcggttagg cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg
    32761 aacttattga tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc
    32821 agggcgatat tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt
    32881 caaatgcgta taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg
    32941 cactgcaaca ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt
    33001 acaacaaact gttcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta
    33061 cggtggcgaa aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg
    33121 cggatgcacc gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg
    33181 atcgccgtga cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg
    33241 aaggggcctc taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg
    33301 tgtcggcgct gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc
    33361 aatcgcaact ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc
    33421 aaggtttagc ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg
    33481 cggtgcgggc gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc
    33541 agaaaggcga tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc
    33601 acagcagcaa caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga
    33661 tccagcaggg cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc
    33721 agcaggcgcg taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg
    33781 cgatggcgcg aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg
    33841 acagcggcaa cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag
    33901 aaaaagctga agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata
    33961 tcgaacgcag cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg
    34021 gcaaatgggc gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg
    34081 tatggattac ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg
    34141 atacgttaat gcgcaatctg gcgcagcaga agccgaacga cccggagcag gtttacgctt
    34201 acgggctgta cctctctggt catgaccagg acagagcggc gctggcgcat atcaacagcc
    34261 tgccgcgcgc gcagtggaac agcaatattc aggagctggt taatcgactg caaagcgatc
    34321 aggtgctgga aaccgctaat cgcctgcgag aaagcggcaa agaagctgaa gcggaagcga
    34381 tgctgcgtca gcaaccacct tccacgcgta ttgacctcac gctggctgac tgggcgcagc
    34441 agcgacgtga ttacaccgcc gcccgcgctg catatcagaa tgtcctgacg cgggagccaa
    34501 ctaacgccga tgccattctg gggctgacgg aagtggatat tgctgccggt gacacagcgg
    34561 cggcacgtag ccagctggcg aaactgcccg ctaccgataa cgcctcgctg aacacacagc
    34621 ggcgcgtggc gctggcgcag gcgcagcttg gagataccgc agcggcgcag cagacgttta
    34681 ataagttgat cccgcaggca aaatctcagc caccgtcgat ggaaagcgcg atggtgctgc
    34741 gcgacggtgc gaagtttgaa gcgcaggcgg gcgatccaaa gcaggcgctg gaaacctaca
    34801 aagacgccat ggtcgcatcc ggtgtgacta cgacgcgtcc gcaggataac gacaccttta
    34861 cccgactgac ccgtaacgac gagaaagatg actggctgaa acgcggcgtg cgcagcgatg
    34921 cggcggacct ctatcgccag caggatctta acgtcaccct cgagcatgat tactggggtt
    34981 cgagcggcac cggtggttac tccgatctga aagcgcacac taccatgttg caggtggatg
    35041 cgccgtattc tgacgggcgg atgttctttc gcagtgattt cgtcaatatg aacgtcggca
    35101 gtttctccac taatgccgat ggtaaatggg atgataactg gggcacctgt acattacagg
    35161 actgtagcgg caaccgcagc cagtcggatt ccggtgccag cgtggcggtc ggctggcgaa
    35221 atgacgtctg gagctgggat atcggtacca cgccgatggg cttcaacgtg gtggatgtgg
    35281 tcggcggcat cagttacagc gatgatatcg ggccgctggg ttacaccgtt aacgcccacc
    35341 gtcggcccat ctccagttct ttgctggcct ttggtgggca aaaagactcc ccgagcaata
    35401 ccgggaaaaa atggggtggc gtacgtgccg acggtgtggg gctaagtctg agctacgata
    35461 aaggtgaagc aaacggcgtc tgggcatcgc ttagtggcga ccagttaacc ggtaaaaatg
    35521 tcgaagataa ctggcgcgtg cgctggatga cgggctatta ctataaggtc attaaccaga
    35581 acaatcgccg cgtcacaatc ggcctgaaca acatgatctg gcattacgac aaagatctga
    35641 gtggctactc actcggtcag ggcggttact acagtccgca ggaatacctg tcgtttgcca
    35701 taccggtgat gtggcgggag cgcacggaaa actggtcgtg ggagctgggg gcgtctggct
    35761 cgtggtcgca ttcacgcacc aaaaccatgc cgcgttatcc gctgatgaat ctgatcccga
    35821 ccgactggca ggaagaagct gcgcggcaat ccaacgatgg cggcagcagt cagggcttcg
    35881 gctacacggc gcgggcatta cttgaacgac gtgttacttc caactggttt gttggcacgg
    35941 caattgatat ccagcaggcg aaagattacg cacccagcca tttcctgctc tacgtacgtt
    36001 attccgccgc cggatggcag ggtgacatgg atttaccgcc gcagccgctg ataccttacg
    36061 ccgactggta agttttcaga tagcgcctct cttaatgccg ctgcgatcgg gtatactcgg
    36121 gcggcaatct gggatttccg gggggagaca atttgcgcgt aagtcgctcg ttaacaatca
    36181 agcagatggc aatggtggca gccgttgtcc tggtgttcgt ttttattttt tgcaccgttt
    36241 tgctgttcca tctggtccag cagaatcgct ataacacggc tacgcaactg gaaagcattg
    36301 ctcgctctgt ccgcgaaccc ttatcttcag ctattttgaa aggcgatatt cccgaagcgg
    36361 aagctattct tgccagcatt aaaccggcag gcgtggtcag ccgtgccgat gtagtgctgc
    36421 ctaaccagtt ccaggcgctg cgtaaaagtt ttattccaga gcgcccggtg ccggtaatgg
    36481 ttactcgcct gtttgagcta ccggttcaaa tctcgctggg cgtttactcg ctcgaacgtc
    36541 cggcaaaccc gcagccaatt gcctatctgg tactacaggc ggattccttc cgtatgtata
    36601 agttcgtgat gagcaccctc tcaacgttag tgaccattta cttacttttg tcgcttatcc
    36661 tgaccgtcgc catcagctgg tgcattaacc gcctgatttt gcatccgtta cgcaatattg
    36721 ctcgcgaact taacgccatc ccagcccagg agcttgttgg tcaccaactg gcattaccgc
    36781 gtctgcatca ggacgatgaa atcggtatgt tggtgcgcag ttacaacctc aaccagcaat
    36841 tgctgcagcg ccattatgaa gaacagaacg aaaatgcgat gcgcttcccg gtgtcggatt
    36901 tgccgaacaa agccttgctg atggagatgc tggagcaagt tgtcgcgcgt aaacaaacca
    36961 ccgcgctgat gatcatcacc tgtgaaaccc tgcgtgatac tgcgggcgtg ctgaaagagg
    37021 cgcaacgaga aattctgctg ctgacgctgg tggaaaaact caaatcggta ctgtcgccac
    37081 gtatgatcct cgcgcagatt agcggttatg actttgctgt cattgccaac ggtgtacagg
    37141 aaccgtggca cgcaatcacc ttaggtcagc aagtgctcac tatcatgagc gagcgcctgc
    37201 cgattgaacg tattcaactc cgtccgcact gtagcattgg cgtggcgatg ttctacggcg
    37261 atctcaccgc cgaacagctt tacagtcgcg ctatttctgc ggcatttacc gctcgccata
    37321 aaggcaagaa tcagattcag ttctttgatc cgcagcagat ggaagccgcc cagaagcggt
    37381 tgacggaaga gagcgatatc cttaatgcac tggaaaatca tcagtttgct atttggttac
    37441 agccacaggt cgagatgacc agcggaaaac tggtcagtgc ggaagtgtta ctgcgtatcc
    37501 agcaaccgga tggcagttgg gacctgccgg atggcttaat cgatcgcatt gagtgctgtg
    37561 ggctgatggt taccgtcggt cactgggtgc tggaagagtc ctgtcgattg cttgcagcct
    37621 ggcaagagcg cggcattatg ctgcccttgt cggtaaacct ctctgcgctg caactgatgc
    37681 acccgaatat ggtggcggat atgctggaac tgttaacccg ctatcgcatt cagccgggaa
    37741 cactgattct ggaagtgaca gaaagccgac gtattgacga ccctcatgct gcggtggcaa
    37801 tcctccgtcc gctgcgcaat gccggagttc gggtggcgct ggatgatttc ggcatgggct
    37861 acgcagggct gcgtcagctg cagcatatga aatcgttgcc aatcgacgta ctaaaaatcg
    37921 acaaaatgtt tgttgaaggc ttgccggaag atagcagcat gattgctgca attatcatgc
    37981 tggcgcagag cctgaactta caaatgattg ccgaaggcgt ggagactgaa gcacaacgcg
    38041 actggctggc aaaagcgggc gttggtattg cccagggctt cctttttgct cgcccactcc
    38101 ctattgaaat cttcgaagag agttacctgg aagaaaagta gctaccccaa actgattaca
    38161 aaactttaaa aagtgctggt ttgtgcgagc cagctcaaac tttttaacct ttttgtttca
    38221 attatgatcc aggtacattt ctgtgatgtt gtctgggtgt tattttaagg ccgcaggtac
    38281 cccataacct tacaagacct gtggttttac taaaggacac cctatgaaaa cctctctgtt
    38341 taaaagcctt tactttcagg tcctgacagc gatagccatt ggtattctcc ttggccattt
    38401 ctatcctgaa ataggcgagc aaatgaaacc gcttggcgac ggcttcgtta agctcattaa
    38461 gatgatcatc gctcctgtca tcttttgtac cgtcgtaacg ggcattgcgg gcatggaaag
    38521 catgaaggcg gtcggtcgta ccggcgcagt tgcactgctt tactttgaaa ttgtcagtac
    38581 catcgcgctg attattggtc ttatcatcgt taacgtcgtg cagcctggtg ccggaatgaa
    38641 cgtcgatccg gcaacgcttg atgcgaaagc ggtagcggtt tacgccgatc aggcgaaaga
    38701 ccagggcatt gtcgccttca ttatggatgt catcccggcg agcgtcattg gcgcatttgc
    38761 cagcggtaac attctgcagg tgctgctgtt tgccgtactg tttggttttg cgctccaccg
    38821 tctgggcagc aaaggccaac tgatttttaa cgtcatcgaa agtttctcgc aggtcatctt
    38881 cggcatcatc aatatgatca tgcgtctggc gcctattggt gcgttcgggg caatggcgtt
    38941 taccatcggt aaatacggcg tcggcacact ggtgcaactg gggcagctga ttatctgttt
    39001 ctacattacc tgtatcctgt ttgtggtgct ggtattgggt tcaatcgcta aagcgactgg
    39061 tttcagtatc ttcaaattta tccgctacat ccgtgaagaa ctgctgattg tactggggac
    39121 ttcatcttcc gagtcggcgc tgccgcgtat gctcgacaag atggagaaac tcggctgccg
    39181 caaatcggtg gtggggctgg tcatcccgac aggctactcg tttaaccttg atggcacatc
    39241 gatatacctg acaatggcgg cggtgtttat cgcccaggcc actaacagtc agatggatat
    39301 cgtccaccaa atcacgctgt taatcgtgtt gctgctttct tctaaagggg cggcaggggt
    39361 aacgggtagt ggctttatcg tgctggcggc gacgctctct gcggtgggcc atttgccggt
    39421 agcgggtctg gcgctgatcc tcggtatcga ccgctttatg tcagaagctc gtgcgctgac
    39481 taacctggtc ggtaacggcg tagcgaccat tgtcgttgct aagtgggtga aagaactgga
    39541 ccacaaaaaa ctggacgatg tgctgaataa tcgtgcgccg gatggcaaaa cgcacgaatt
    39601 atcctcttaa tctcatcact tatgcccgca ctcccttccc cgagtgcggg tgtttgcgca
    39661 taattgaccc ctgtgcctga acttaccacc tgggtgagtt caggtttatt tcacttcttc
    39721 cgtgacattt tcatgttctt gcggtctaac acgaagtgtt tttaacgtca tttagactga
    39781 ccgacaacgg gcagtttttt tattaccagg atagttgatc aggggttcac atgcagggca
    39841 caaaaattcg acttttagcg ggcggtttgc tgatgatggc cactgctggc tatgtgcagg
    39901 cagatgcgct ccagcctgat ccagcatggc aacaggggac gctttccaac ggtttacagt
    39961 ggcaagtgct gaccaccccc cagcgtccca gcgatcgtgt tgaaattcgc ctgctggtta
    40021 ataccggttc gctcgccgaa agtacacaac agagcggtta cagtcacgcc atccctcgta
    40081 ttgcgctaac gcaaagcggt ggccttgacg cagcacaggc gcgttcattg tggcagcagg
    40141 ggatcgaccc taaacgcccg atgccgccgg taattgtctc gtatgacact acgctgttta
    40201 atctgagttt gcccaataac cgtaacgatt tgctgaaaga ggcgctctct tacctggcaa
    40261 atgccactgg caaactgact atcacgccag aaaccatcaa ccacgcgctg caaagtcagg
    40321 acatggtggc aacctggcct gccgatacta aagagggctg gtggcgttat cgtctgaaag
    40381 gatcaacctt gttaggtcac gatcctgccg atccgctgaa acaacccgtt gaagcggaaa
    40441 aaattaaaga tttctatcag aaatggtaca ccccggatgc aatgacgcta ctggtggtgg
    40501 gaaacgtgga tgcgcgctcg gttgtcgacc aaatcaacaa aacgtttggc gaactgaaag
    40561 gcaaacgtga aacgccagct ccggtgccga cgctttctcc gctgcgtgcg gaagcggtga
    40621 gtattatgac tgacgcggtg cgtcaggacc ggttatctat catgtgggat acgccgtggc
    40681 agccgattcg tgaatcagcc gcactgctgc gctactggcg tgcggacctg gcccgcgagg
    40741 cgctgttctg gcatgttcag caagcgttaa gcgccagtaa cagcaaagac atcggtcttg
    40801 gatttgactg ccgtgtgctg tatctgcgtg cgcagtgtgc catcaacatc gaatcaccaa
    40861 acgacaagct gaacagcaac cttaatctgg tggcgcgtga actggcgaag gttcgcgata
    40921 aaggtctgcc ggaagaagag ttcaatgcgt tagtggcgca aaagaaactg gagctgcaga
    40981 aactgtttgc cgcctatgcg cgggctgata ccgatattct gatgggtcag cggatgcgtt
    41041 cgttgcaaaa tcaggtcgtc gatatcgcgc cggagcagta tcagaaactg cggcaggatt
    41101 tccttaacag cctgacggtg gagatgttaa atcaggatct gcgtcagcag ttgtcgaatg
    41161 atatggcgtt aatactgctg cagccgaaag gcgagccgga atttaacatg aaagcgttgc
    41221 aggcggcctg ggatcaaatc atggccccat ctaccgccgc tgcgaccacc tctgtcgcca
    41281 cggatgacgt acatcctgaa gtgacggata ttccacctgc acagtaatgc ctgatggtga
    41341 tgagttagca caatcatcag gtatttatgg atgagctgga tagaggggta acactttatc
    41401 cagccttttg catatgctgc ttttacgctg gcatcgcctc acgcgggata atcgcgccgc
    41461 gatactgaat aacggtactt gcggtcaggt gcccgcgttt cgccgcgttt tccgcactgc
    41521 cgcccgttaa acgtaccgcc agataaccgg cactgaaaga gtcgccagct gcggtggtat
    41581 cgatcacttt ttctttcggc agtttcaccg ccggaacatc cactaaccct tcgccagcaa
    41641 tggacacaag gcaagaatcc gccccgcgtt tcaccaccac ttctttcacg cccgcgttat
    41701 gggtgcgcgc aatgacgtct tccaccggct gttgacccca cagcgcgtct tcgtcgtcca
    41761 gcgtcaggaa ggcgatatcc gtgcattcca gcatttgttg gtacacctgc tgtgtctctt
    41821 ctttgctggc ccacaggcgc ggacgatagt tattgtcgaa aatcactttt ccgccgttgg
    41881 cgcggcattc gcgcagcagg gaaagcagct tttcgcggct ggtcgggctt aagatcgcca
    41941 ggctaatccc gctcaggtag agataatcga aattcgccag ctcttcgcaa atcgccgcag
    42001 actgctcgct ctccagccag aatttggcag cggcttcgtt acgccagtag tagaacgtac
    42061 gttcaccggt gctgtcggtt tcaatgtagt aaaggcccgg cagacggttt tccatccgtt
    42121 gggtcaggga agtatcaacg ttctcgccgt gccaggcgtc cagcatttgc tggctaaaac
    42181 tgtccgttcc cagcgccgtt acgtaatgga cggttaatgc cgcaggatcg acctgacggg
    42241 cgatatagac ggaagtgttc agggtatcgc cgccgaaacc gcgcttaacg tccgcgcctt
    42301 tctcggaaag ctcaatcatg cattcgccaa tcacggcaat ctttttggac atagtcgtga
    42361 acctgatctg taaaaaatta gcgttagttt gcgctgtggt ggtttgctgg tcaaccatat
    42421 taaaacagtg ttccattatt tttttgagcc cggacgtatt cctgaaagat tccgttgtgg
    42481 agtgaggaaa tttaccccgg ccaggcggta aagttctgcc cttacgcgcc gataatcttt
    42541 gtcgagtccg ggcagcatca cttttaaaca caggacatct ttgatgataa ggcaggttat
    42601 ccagcgaata agcaaccctg aagcaagcat cgagagcttg caggaacggc gtttttggtt
    42661 gcagtgtgag cgtgcttaca cctggcagcc gatctatcaa acatgcgggc ggttaatggc
    42721 cgtggagcta ttaacggtgg tcgcgcatcc cttgaaccct tcgcaacgcc tgccgccgga
    42781 tcgctatttt actgaaatca ccgtcagcca tcggatggag gttgtgaaag agcagattga
    42841 tttgctggcg caaaaagccg acttctttat agagcacggc ctgctggcat cggtcaatat
    42901 tgatggccct acgctcatcg ccctgcgtca gcaaccaaaa atcctgcgcc agattgagcg
    42961 tcttccctgg ctgcgtttcg aactggtgga gcatatccgt ctgccgaaag attcaacctt
    43021 tgcctcgatg tgtgaatttg gcccgctgtg gctggatgat tttggtaccg ggatggcaaa
    43081 tttctctgca ctaagtgaag tgcgttatga ctacatcaaa atcgcgcgag aactgtttgt
    43141 gatgctgcgt cagtcgccgg aaggacgcac actcttttct cagcttttac atctaatgaa
    43201 tcgctattgt cgcggggtga ttgttgaggg cgtagaaacg ccggaagagt ggcgtgatgt
    43261 tcagaactcg cccgcattcg ccgcacaagg ctggtttctt tcacgcccgg caccgataga
    43321 aacgctgaat acggcggttc tggcgctata agctgcctca ttttccgcct ggctggacta
    43381 tctttaggac tggcacagga agaatatgag gaagcgaacg atgagcaagg caggcaaaat
    43441 aaccgctgcg atttcagggg ctttcttgtt gttgattgtc gtggcgatca ttttgattgc
    43501 aacttttgac tggaatcgac tcaaaccgac catcaaccag aaagtctctg cggagttgaa
    43561 tcgtccgttc gctatccgtg gcgatctggg cgtggtgtgg gagcggcaaa agcaagaaac
    43621 aggctggcgc agctgggtgc cgtggcccca tgtacacgcg gaagacatca ttcttggcaa
    43681 tccaccggat attcccgaag tcacgatggt gcatttgcca cgcgtagagg caacgctggc
    43741 cccgctggcg ctgctgacca aaacggtctg gctgccgtgg atcaagctcg aaaagcccga
    43801 cgcgcgcctg attcgcctct ccgaaaagaa caataactgg acgtttaatc tcgccaacga
    43861 tgataacaaa gacgcgaatg caaagccgtc ggcatggtcg tttcggctgg ataatattct
    43921 tttcgatcaa gggcggatcg ccattgatga caaagtaagc aaagcggatc tggagatttt
    43981 tgttgatccg ttaggcaagc cgctgccgtt cagcgaagtt actggatcga aaggtaaagc
    44041 ggataaagaa aaggtgggcg attacgtttt tggcctgaag gcgcagggac gttataacgg
    44101 tgaaccgctc acgggtacgg gaaaaatagg cggtatgctg gcgctgcgtg gcgaagggac
    44161 gccgtttccg gtacaggctg atttccgctc tggtaacacc cgtgttgctt ttgatggcgt
    44221 cgtgaatgac ccaatgaaga tgggcggtgt cgatttacgg cttaaatttt ctggcgattc
    44281 actgggtgat ctctatgaac tgacgggcgt tctgctgccc gataccccgc cgtttgaaac
    44341 ggatggtcgg ctggtagcga aaatcgacac tgaaaaatcg tcggtctttg attatcgcgg
    44401 ttttaatggg cgaattggcg atagcgatat ccacggttct ctggtctaca ccaccggaaa
    44461 gccacgacca aaactggaag gtgatgtcga gtcgcggcaa ttgcggctgg cggacctggg
    44521 accgttaatt ggcgttgact ccgggaaagg ggcagaaaag tcgaaacggt ctgaacagaa
    44581 gaagggcgaa aaaagcgttc agcctgcggg caaagtgctg ccttatgacc gcttcgaaac
    44641 cgataaatgg gacgttatgg atgccgatgt tcgcttcaaa gggcggcgca ttgagcatgg
    44701 cagtagcctg ccgattagcg atctttctac tcatatcatc ctcaaaaatg ctgacctgcg
    44761 cctgcaaccg ctgaaatttg gcatggcggg cggcagcatt gcggcgaata ttcatctgga
    44821 aggcgataaa aagccgatgc aggggcgggc agatattcag gctcgtcgac tgaaactgaa
    44881 agaactgatg cccgatgtgg aactgatgca gaagacgctg ggggaaatga acggtgacgc
    44941 ggaactacgc ggtagcggta actcggtggc agcactttta ggcaacagta acggcaacct
    45001 gaaactgttg atgaatgacg ggctggtgag ccgcaacctg atggagattg ttgggctgaa
    45061 tgtcggcaac tacattgtcg gtgcgatatt tggtgacgat gaggtgcggg tgaactgcgc
    45121 ggcggcgaat ctgaatattg ccaacggcgt ggcgcgcccg cagatttttg ctttcgatac
    45181 tgagaacgcg ttgattaatg ttaccggcac ggcaagtttt gcttcggaac agctggattt
    45241 gactattgat ccggagagta aagggattcg gattatcaca ctgcgttcgc cgctgtatgt
    45301 gcgtgggacg tttaaaaatc cgcaggctgg ggtgaaagcc gggccgttga ttgcccgtgg
    45361 tgctgttgct gcggcactgg caacgctggt aacaccggcg gcggcgttac tggcactgat
    45421 ctcaccttcc gaaggggagg ctaatcagtg tcggacgata ttgtcgcaga tgaagaagtg
    45481 atgaggttgg attgtgtttg ctggtgagtt cgggagcagc gtgttcccct caccctaacc
    45541 ctctccccaa aggggcgagg ggactgtttt gtgcgtgttg aaacaacgag taccgctttt
    45601 ccgttcactt tctgggggag ggctgttatg ttcgcgatat aacatctcgc tccgcttttc
    45661 tccctctccc tttcagggag agggctgggg tgagggtggc tgcccacacg atacttacaa
    45721 cgactgatgt cgcgtctcat gggtcagcag cagggcgatt aacgtcaagc cagccatcgc
    45781 cgccagatat aaccccaccg cacctaaccc gtagttagcc tgcaaccagg ctgcgatata
    45841 tggtgcaacg gaagccccga gaatcgacgc tacgttgtaa gagaacgatg ctccggtgta
    45901 acgcacttct gtcgggaaca gctctggtaa cagcgcgccc atcggcccga aggtcagtcc
    45961 catcagactt aaccccagca gcaggaaggc aaaaaccaga atcgggttgc cagaaccgag
    46021 cagcgggtta aaggcgaaca gcgcgaacag gatgatcagc gtggtgatga ttaccatgct
    46081 tttacggcga ccaaaggcat cagccagtaa tccggcgact ggcaccatca cgccaaaacc
    46141 aataactgcc atcatcagca tccacaacac ttcgttacgc ggcaggccaa gcccaactgg
    46201 cgcggcggcg gtactgaagg tcatagagta gaccgtcatg atgtaaaaca gcgtataggt
    46261 tgccagcata atgaacgtac ccagtacggt tacgcgaaca tgtttggtca gcagcgtacc
    46321 cagcgggatc ttcacctgtt ttttcgcttt agcgactttc tcaaacaccg gcgactcatg
    46381 cagcgacacg cgaacataca ggccgataat gaccagcacc gccgagaaga taaatggcac
    46441 acgccagccc cagctcataa actgctcgtc ggtcagtagc caggaaagca gcaagaaagt
    46501 gccgttggca aagaagaagc caatcggtgc gcccagctgt gggaaggagc catacagtgc
    46561 acgtttgcgc ggtggggcgt tttcagtcgc cagcagcgcc gcgccgcccc attcaccgcc
    46621 taagcccaga ccctgaccaa atcgagccag cgccagcagc agcggggcga aaataccaat
    46681 cgtggcatag cccggcagca gaccaatcac cacggtcgaa atccccatcg ttagcaacga
    46741 ggcgaccagc gtcgctttac gcccaacgcg atcgccaaaa tgaccaaaaa cggcagagcc
    46801 aatggggcgc gcgacgaagg cgatggcgaa ggtggcgagc gactgtagcg ttgctgccgt
    46861 tggatcgccc tgcgggaaga agatatgcgg aaacacaata acggccgcag tggcgtaaat
    46921 gtaaaagtcg aagaactcaa tggccgtgcc aatgagagag gcgacaagga ctttattacg
    46981 cgagttgatt ggcgtgtatt cttgctcgtg gtcgagtgtt gtggctgttg cttgcataat
    47041 cttttcttat tttttggcta acgaatagcc atattacgca cagcaaaagt gatatttcaa
    47101 tgtgtaacaa atcgcggaaa tggtcaaaaa ccaggcaaaa aggggataat gtttaggcca
    47161 gtgatttttc ttctatgaaa tgcttcacat aattttataa atagtggata aaactggttt
    47221 caggttaaat aaaagttacc aactggattt atatgctggg gttatgagtt ggaatgaaat
    47281 tgtttgctcc caaatagatg gctgaagagg ctctggggaa gagtttcaag aggttatgtg
    47341 cgcattatag ccggatgcga tgctggcgca tcttatccgg cctacgggga gccaggccga
    47401 ataaaatcgt atgccggata aggcgtttac gccgcatccg gcctatttta aggctgcgtt
    47461 ttccctggca ttcgcgggtc gtctttatat tcggcggtgg caatccacgc cgcgcaaaac
    47521 agcgtcaaac gggcgaagaa gtagaaaaat gccatcagcc ccagcacgga accaaatgct
    47581 gcgccagagg gggatttcat cagcgatggc aaggtgtagg tcatcacgat tttaatcact
    47641 tcaaaaccaa tagcagccag aaatgttccg cgaatcaggg cttttttgcg cggacggtgg
    47701 cgcggcaacc gccagaagat ccagaagaaa agcagatagt tggcgaagat ggaaatcgcc
    47761 aggccaatca accgccacgt cggtttcagc cactcaatgc tgttcaggtg cagggcgcta
    47821 ataatcattt gctgcgccga accggcaacc gaggtgatcg acagcgtcac aatcagcgca
    47881 attaacaaac caatcagtga aataaaatca cgcagatatt ttacccagaa cttctcctga
    47941 tcttgcggcg agcgttccca gacatcgcgc gactgggcac gaatcgcttc acgcaggtta
    48001 cccatccagt tgatgccaga ataaagcgcc accgccaggc cgacaagccc tacagtcgta
    48061 cgctgctgaa cggcggtgtt gatggtgttt ttcaacgtgg cggctagcgt cggatcgctg
    48121 atgttttgca gaattttgtc gaagatatcc tgtagcaaca tcggatggga ggccagcaca
    48181 aagccccctg cggcaaacga caccatcaaa atcgggatca tcgataaaaa tgagaaatag
    48241 gtgatagccg caccaaactg gttacccagc cgatcgttaa agcgttctgt cgcgcgaatc
    48301 aggtgcgcaa taaccggttg gcgttggact ttttcggcag tggtggtgac ggtttccagc
    48361 gcctggctaa ctttgccgcc tttctcgcta ttatccaact gcttaatcgg ctcgtgctcc
    48421 agatcctggg tgggacgttt gatctcgttt tcctgcgtca tccgctcttt tgtccttctg
    48481 tcgtggtcat tcttaaaaag tatagtcggt cagtccaggt gctctttcat tacgccagcc
    48541 agccactgca taaacaggtt tacacgccgg gaaagctccc gacgctgcgg gtaaaccagc
    48601 gaaagggaga gcggctcggc acggtagcct ggcaacactt caataagccg cccggcacgc
    48661 agggcttcgc gcacggcaat gcgcgggatc tgaataatcc ccagaccggc aagaccggcg
    48721 gcgagatagt tttcgctact gtttaccgtc aacatgccgc cagacttaaa ccactggacg
    48781 ccattaacgc tggcaacctc aaaacctaac ggatgtacac ccaggtgcgg tgtataacgc
    48841 actattgcgt gtgaagtcag atcatcgggg ctttgcggat aaccaaagcg cgtcagatag
    48901 tgcggactgg cacagttgac catggtcagt ttgccgaggg gacgggcgat aacgccatct
    48961 tccggtaacg cgccagtgcg tatcacgcaa tcaaaaccat catgaagaat atctaccgga
    49021 cggtcatggc tactcagttc cagctcaatt cccggatgca gatagagaaa ttccgacagg
    49081 cgcggcagta acaggctttt cgcgattcct ggcgggatgt cgatgcgtaa tttaccgctg
    49141 atactggtgg catcctgttg aaacagaccg tccagttcgc tgagattact caacacatct
    49201 tttgctcgtt gataataggt catgccttct ggcgtgagtt tgacccgtcg cgtggtgcgc
    49261 tggagaagct gggtgccaag ttgatgttcc agtgcctgta tctggcgcga aacacttcct
    49321 tttggcaaag caaagaaatc cgccgcgcgg gaaaaacttt ccagctccgc gactttgatg
    49381 aacaactgca ttgcgtgaat tttatccatt tctttgaccg attgttgttc acagcgaaac
    49441 agtaacgcgc atatcgcatt gtttattgtt ttttctaaca tctaatgccg ggtttcacaa
    49501 tgttgtcatg acgaatgtgg cgatttctca gcaggaaaag ggcggaatag ctttctgaga
    49561 aaggtgaatc cccctaaaca gagcgtattt ttcagcgatt ccctttaaag tgaatagagg
    49621 acgtgttggc tatttgtgct tatttttgtc tcattgccgt ttgttatttt tgcttgtggt
    49681 cgcggagttt catttttaat tgcatgcgta ctaaccacca atataaggaa atcctcaaaa
    49741 ttgttttaag tagtgccctc gacatgtaat ttcaggcgtt tagctgtaac tatttatatc
    49801 ggtaactatt ttctacgcgt catctctgta gaatggccac cgccaaatgg cgaattctta
    49861 ttttttctcg cgggtaatac ccttccacta cttaagtctt tttaacgtat tcaggtggag
    49921 attcagttat gcaaatagtc atgtttgaca ggcagtcaat atttattcat ggaatgaaaa
    49981 tcagtttaca gcagcgtatt ccaggagtga gtattcaggg ggccagtcag gcagacgagt
    50041 tatggcaaaa gctggaaagt taccctgaag ccttagttat gctcgatggc gatcaggatg
    50101 gtgagttttg ctactggttg ctgcaaaaaa ccgtggtgca gtttcctgag gttaaggtgt
    50161 taatcacggc gacggattgc aataaacggt ggttacagga agttattcat tttaatgtgc
    50221 tggccattgt gcctcgtgat tcaaccgtcg agacatttgc gttggcggta aatagtgcgg
    50281 cgatggggat gatgtttctg cctggagact ggcggacgac gccggaaaag gacattaaag
    50341 atctcaaatc gttaagcgcc cgccagcggg agattttaac catgttagcg gcgggcgaat
    50401 caaataagga gatcggcaga gcgctgaata tcagtaccgg aacggtaaaa gcgcatctgg
    50461 aatccctgta tcgccgtctg gaagtgaaaa atcgcaccca ggcggcaatg atgttaaata
    50521 tctcctcctg aagaggataa ttttacagcc agcaggaagt tagcggctgg ctgtaaaaat
    50581 attatggttc gccgtacaaa ccaattaaac ggcgtaccac accgttagtc cagccaaacc
    50641 catcctgcaa cggatactcg ccaccgccgc cttcgcgggg aacaccatcg gcaatatggt
    50701 atttctcgat cattttgtgc tgttccagat agaactgatt caccgttttc agccagctgc
    50761 gcgcgatttc atcacccaga aggtcatcgc catacatttt aaatccctga attgccatcc
    50821 attgtaacgg tgcccagcca ttgggtttat cccactgttc accggtttcg tactcgcttg
    50881 ccagaatccc gccaggtgtc agtaaccggc tgcgtacggc gtttgccaga cgatcggcct
    50941 gttcatggtt cgccatgccg acatagagcg gcacaatggc ggcagcggaa aacagcgcca
    51001 gttgttcgcg tcgccagtcg taatcgcggt agatgccgtt ttcatcatcc cagaggtaac
    51061 ggtttaccgc atcgcgacgg gcactggcct tctggcggaa cagcgcttct gtctctttct
    51121 cgcctttcag cgccgagatg ttggcgatgg cgctctccag tttaaacagg aaggcattca
    51181 gatcgatggg gatgaactgg gtggtacgaa tgctcgccag acgaccagta tcacgcagcc
    51241 aacgggaaga gtaatcccaa cctgaggccg ctcccgcgcg taaatcgcgg tacacctcgt
    51301 tgggcgggcg accagaatgt ttcgcggttt caacgtcctc aagccaggat tcgtcacgcg
    51361 gcgtgtcgcg atcgtcccaa taacggttga gcagcgatcc gtccggcatc cgcacaacat
    51421 ggcgataggc ctgattaggg atcaacgatt ctgcaccgtc catccagaag gcatattcca
    51481 ttttaaggtg gtcgagatag cggcgcgcac cgcgtacacc atcttcttca aacaactcca
    51541 ccatcagcgc aaaaaccggt ggttgcgatc ggctcaaata ataggtgcgg ttgccgttgg
    51601 ggatgtgacc atagttttcg atcatccagg cgaagttatc ggccatgcat ttcagcaaat
    51661 cttcccgacc actttccgcc agccccagca tggtgaaata ggaatcccaa tagtaggttt
    51721 cgctaaaacg gccgcccggg acaatatatg actgcggtag cgccagcaga gaagaccacg
    51781 gaatgtgatc ctgtggttcg cgggttagca ccggccacag ctggtcgata tgctctttca
    51841 gggaattttg cgggtccgat acatactcgc tggagtagac ctccggcagc cagaagtgat
    51901 tttcaacaaa cttgcgcaag tcaaaatcac gatgacggcg cactttacgg tagcggatta
    51961 aaatatccag cgggtccatt ttgggtgcgc agtcgggaaa ggttttactg tcggggaaaa
    52021 ttttcgccga ctgaacatgc tcgaacagtt cgagatagcg atcggcaggc gtcagcgcat
    52081 cagaggcggg cagcccttca atcatctcgg gttccggttc tgcctcgatc atctcatcca
    52141 gttttaattc atacgggtcc agctcatagc agagatcgac ttcgatcatc agttcgtctg
    52201 gattagggtt ttgaattttc tgattgagca taaggatcca acctccgaaa ttcgtcgcta
    52261 attatcgggc ttttctttaa gggtagtcat tcgctccgcc tggtgggcga caaagcgtgc
    52321 ggtggatcac gtttattaac ctgtcaaaaa atataacgca ctaatatcca tggaatttat
    52381 aaaaatacgg atttcgtgtt gtttttcatt ttcagaacat tcaatcctgc tctcgccgca
    52441 atcatgatat gccattcctg atctgggtaa taggcacagg ctatcttatt gatagtttat
    52501 attcatgtaa ttgattgatt cctatcaaaa agcgtatctc cctgtttttt atgatggctt
    52561 tgtctcgcag agacgtttgc agcattctgg aaaattttct gggtcacaat aactcaccac
    52621 cgtgagggaa gaataatgaa aatggtctca cgtattaccg cgatcggcct ggctggcgtc
    52681 gcgatttgct atttagggtt atctggttat gtgtggtacc acgataataa acgcagtaaa
    52741 caggccgatg ttcaggcatc tgctgtcagt gaaaataata aggttttagg ctttctccgc
    52801 gaaaaaggat gcgactattg ccacacacct tcggcagaat tacccgccta ttattatatt
    52861 cctggcgcga aacagttgat ggattacgac attaagcttg gatataaatc ttttaacctc
    52921 gaggccgtgc gtgcggcact gctggctgat aaacccgttt cgcaaagcga tctgaataag
    52981 attgaatggg tgatgcagta tgaaactatg ccaccaacgc gttataccgc gctacactgg
    53041 gcgggtaagg tgagtgatga agagcgggcg gaaatactgg cctggattgc aaaacagcgc
    53101 gcggaatatt acgccagcaa tgatactgct ccggagcatc gcaatgaacc ggtgcagccc
    53161 atcccgcaaa aactgcctac cgatgcgcaa aaagtggcgt tgggctttgc gctgtatcac
    53221 gatccccgtt tatcggctga tagcaccatt tcatgcgctc attgccatgc gttgaatgcg
    53281 gggggcgtcg atggcagaaa aacatcgatt ggtgttggtg gcgcagttgg gccgattaac
    53341 gcgccgacgg tatttaactc agtatttaac gttgagcagt tctgggatgg tcgtgcggca
    53401 acattgcagg atcaggcagg tggaccgccg ttgaacccga ttgaaatggc gtcgaaatcc
    53461 tgggacgaaa ttattgctaa gctggaaaaa gatccgcagc ttaaagcgca gttcctcgaa
    53521 gtttatccgc aaggtttcag tggcgaaaat attactgatg ccattgctga atttgagaaa
    53581 acattaatta cgccggattc cccatttgat aaatggttgc gtggagatga aaatgcgctg
    53641 acggcgcaac agaaaaaagg ctatcaatta tttaaagata ataaatgtgc aacttgtcat
    53701 ggtggtatta ttctcggcgg acgttccttt gaaccgttgg ggctgaaaaa agactttaac
    53761 tttggggaaa ttacggcggc ggatattggt cgtatgaatg tgactaaaga agagcgtgat
    53821 aaattgcgtc agaaagtacc cggtttacgt aacgttgctt taacggcacc gtacttccat
    53881 cgcggtgacg tgccgacgct ggacggggcg gtgaaactga tgctgcgcta tcaggtaggc
    53941 aaagagctgc cgcaggagga tgtggatgat atcgtagctt tcctgcacag tctgaacggg
    54001 gtgtacacgc cgtatatgca ggataaacaa taattaattt gatcgcccga acaggaatgt
    54061 ttgggcgatt tttattacga taataaagtc tgtttttaat attatcatgt taaatgttta
    54121 tattataaaa agtcgttttt ctgcttagga ttttgttatt taaattaagc ctgtaatgcc
    54181 ttgcttccat tgcggataaa tcctactttt ttattgcctt caaataaatt taaggagttc
    54241 gaaatggacc agaagctgtt aacggatttc cgctcagaac tactcgattc acgttttggc
    54301 gcaaaggcca tttctactat cgcggagtca aaacgatttc cgctgcacga aatgcgcgat
    54361 gatgtcgcat tccagattat caatgatgaa ttatatcttg atggcaacgc tcgtcagaac
    54421 ctggccactt tctgccagac ctgggacgac gaaaacgtcc ataaattgat ggatttgtcg
    54481 atcaataaaa actggatcga caaagaagaa tatccgcaat ccgcagccat cgacctgcgt
    54541 tgcgtaaata tggttgccga tctgtggcat gcgcctgcgc cgaaaaatgg tcaggccgtt
    54601 ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg gcgggatggc gatgaaatgg
    54661 cgttggcgca agcgtatgga agctgcaggc aaaccaacgg ataaaccaaa cctggtgtgc
    54721 ggtccggtac aaatctgctg gcataaattc gcccgctact gggatgtgga gctgcgtgag
    54781 atccctatgc gccccggtca gttgtttatg gacccgaaac gcatgattga agcctgtgac
    54841 gaaaacacca tcggcgtggt gccgactttc ggcgtgacct acaccggtaa ctatgagttc
    54901 ccacaaccgc tgcacgatgc gctggataaa ttccaggccg acaccggtat cgacatcgac
    54961 atgcacatcg acgctgccag cggtggcttc ctggcaccgt tcgtcgcccc ggatatcgtc
    55021 tgggacttcc gcctgccgcg tgtgaaatcg atcagtgctt caggccataa attcggtctg
    55081 gctccgctgg gctgcggctg ggttatctgg cgtgacgaag aagcgctgcc gcaggaactg
    55141 gtgttcaacg ttgactacct gggtggtcaa attggtactt ttgccatcaa cttctcccgc
    55201 ccggcgggtc aggtaattgc acagtactat gaattcctgc gcctcggtcg tgaaggctat
    55261 accaaagtac agaacgcctc ttaccaggtt gccgcttatc tggcggatga aatcgccaaa
    55321 ctggggccgt atgagttcat ctgtacg
//
LOCUS       DAPHJE010000010        54069 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPHJE010000010 DAPHJE010000000
VERSION     DAPHJE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.010"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..1371
                     /locus_tag="RH222_000668"
     CDS             70..1371
                     /locus_tag="RH222_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDV3795731.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            1424..2395
                     /locus_tag="RH222_000669"
     CDS             1424..2395
                     /locus_tag="RH222_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV3795732.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(2507..2956)
                     /gene="rplI"
                     /locus_tag="RH222_000670"
     CDS             complement(2507..2956)
                     /gene="rplI"
                     /locus_tag="RH222_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV3795733.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(2998..3225)
                     /gene="rpsR"
                     /locus_tag="RH222_000671"
     CDS             complement(2998..3225)
                     /gene="rpsR"
                     /locus_tag="RH222_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV3795734.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3230..3544)
                     /gene="priB"
                     /locus_tag="RH222_000672"
     CDS             complement(3230..3544)
                     /gene="priB"
                     /locus_tag="RH222_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV3795735.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQVITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3551..3946)
                     /gene="rpsF"
                     /locus_tag="RH222_000673"
     CDS             complement(3551..3946)
                     /gene="rpsF"
                     /locus_tag="RH222_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV3795736.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4273..4548
                     /locus_tag="RH222_000674"
     CDS             4273..4548
                     /locus_tag="RH222_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3795737.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4677..5363)
                     /gene="ulaF"
                     /locus_tag="RH222_000675"
     CDS             complement(4677..5363)
                     /gene="ulaF"
                     /locus_tag="RH222_000675"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV3795738.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(5363..6217)
                     /gene="ulaE"
                     /locus_tag="RH222_000676"
     CDS             complement(5363..6217)
                     /gene="ulaE"
                     /locus_tag="RH222_000676"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV3795739.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(6227..6877)
                     /gene="ulaD"
                     /locus_tag="RH222_000677"
     CDS             complement(6227..6877)
                     /gene="ulaD"
                     /locus_tag="RH222_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV3795740.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(6891..7355)
                     /gene="ulaC"
                     /locus_tag="RH222_000678"
     CDS             complement(6891..7355)
                     /gene="ulaC"
                     /locus_tag="RH222_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV3795741.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(7365..7670)
                     /gene="ulaB"
                     /locus_tag="RH222_000679"
     CDS             complement(7365..7670)
                     /gene="ulaB"
                     /locus_tag="RH222_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV3795742.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(7686..9083)
                     /gene="ulaA"
                     /locus_tag="RH222_000680"
     CDS             complement(7686..9083)
                     /gene="ulaA"
                     /locus_tag="RH222_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV3795743.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9438..10502
                     /gene="ulaG"
                     /locus_tag="RH222_000681"
     CDS             9438..10502
                     /gene="ulaG"
                     /locus_tag="RH222_000681"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV3795744.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            10610..11365
                     /gene="ulaR"
                     /locus_tag="RH222_000682"
     CDS             10610..11365
                     /gene="ulaR"
                     /locus_tag="RH222_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV3795745.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(11362..12111)
                     /gene="yjfP"
                     /locus_tag="RH222_000683"
     CDS             complement(11362..12111)
                     /gene="yjfP"
                     /locus_tag="RH222_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3795746.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGAGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12293..12622
                     /gene="bsmA"
                     /locus_tag="RH222_000684"
     CDS             12293..12622
                     /gene="bsmA"
                     /locus_tag="RH222_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV3795747.1"
                     /translation="MFSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            12771..13046
                     /gene="yjfN"
                     /locus_tag="RH222_000685"
     CDS             12771..13046
                     /gene="yjfN"
                     /locus_tag="RH222_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV3795748.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13163..14788)
                     /locus_tag="RH222_000686"
     CDS             complement(13163..14788)
                     /locus_tag="RH222_000686"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV3795749.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(14872..16035)
                     /locus_tag="RH222_000687"
     CDS             complement(14872..16035)
                     /locus_tag="RH222_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV3795750.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(16038..16676)
                     /locus_tag="RH222_000688"
     CDS             complement(16038..16676)
                     /locus_tag="RH222_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV3795751.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(16686..17084)
                     /locus_tag="RH222_000689"
     CDS             complement(16686..17084)
                     /locus_tag="RH222_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV3795752.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(17102..17761)
                     /locus_tag="RH222_000690"
     CDS             complement(17102..17761)
                     /locus_tag="RH222_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV3795753.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(17812..18510)
                     /locus_tag="RH222_000691"
     CDS             complement(17812..18510)
                     /locus_tag="RH222_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV3795754.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(18529..18930)
                     /locus_tag="RH222_000692"
     CDS             complement(18529..18930)
                     /locus_tag="RH222_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV3795755.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(19057..19788)
                     /gene="rlmB"
                     /locus_tag="RH222_000693"
     CDS             complement(19057..19788)
                     /gene="rlmB"
                     /locus_tag="RH222_000693"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV3795756.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(19968..22409)
                     /gene="rnr"
                     /locus_tag="RH222_000694"
     CDS             complement(19968..22409)
                     /gene="rnr"
                     /locus_tag="RH222_000694"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV3795757.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(22448..22873)
                     /gene="nsrR"
                     /locus_tag="RH222_000695"
     CDS             complement(22448..22873)
                     /gene="nsrR"
                     /locus_tag="RH222_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV3795758.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(23078..24376)
                     /gene="purA"
                     /locus_tag="RH222_000696"
     CDS             complement(23078..24376)
                     /gene="purA"
                     /locus_tag="RH222_000696"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV3795759.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(24480..24677)
                     /locus_tag="RH222_000697"
     CDS             complement(24480..24677)
                     /locus_tag="RH222_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV3795760.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(24759..25763)
                     /gene="hflC"
                     /locus_tag="RH222_000698"
     CDS             complement(24759..25763)
                     /gene="hflC"
                     /locus_tag="RH222_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV3795761.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(25766..27025)
                     /gene="hflK"
                     /locus_tag="RH222_000699"
     CDS             complement(25766..27025)
                     /gene="hflK"
                     /locus_tag="RH222_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV3795762.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(27111..28391)
                     /gene="hflX"
                     /locus_tag="RH222_000700"
     CDS             complement(27111..28391)
                     /gene="hflX"
                     /locus_tag="RH222_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV3795763.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(28468..28776)
                     /gene="hfq"
                     /locus_tag="RH222_000701"
     CDS             complement(28468..28776)
                     /gene="hfq"
                     /locus_tag="RH222_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV3795764.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(28862..29812)
                     /gene="miaA"
                     /locus_tag="RH222_000702"
     CDS             complement(28862..29812)
                     /gene="miaA"
                     /locus_tag="RH222_000702"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV3795765.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(29805..31652)
                     /gene="mutL"
                     /locus_tag="RH222_000703"
     CDS             complement(29805..31652)
                     /gene="mutL"
                     /locus_tag="RH222_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV3795766.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(31662..32999)
                     /gene="amiB"
                     /locus_tag="RH222_000704"
     CDS             complement(31662..32999)
                     /gene="amiB"
                     /locus_tag="RH222_000704"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV3795767.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(33018..33479)
                     /gene="tsaE"
                     /locus_tag="RH222_000705"
     CDS             complement(33018..33479)
                     /gene="tsaE"
                     /locus_tag="RH222_000705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV3795768.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(33451..34998)
                     /gene="nnr"
                     /locus_tag="RH222_000706"
     CDS             complement(33451..34998)
                     /gene="nnr"
                     /locus_tag="RH222_000706"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV3795769.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            34997..36136
                     /gene="queG"
                     /locus_tag="RH222_000707"
     CDS             34997..36136
                     /gene="queG"
                     /locus_tag="RH222_000707"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV3795770.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(36119..36172)
                     /locus_tag="RH222_000708"
     CDS             complement(36119..36172)
                     /locus_tag="RH222_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795771.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(36406..36481)
                     /locus_tag="RH222_000709"
     tRNA            complement(36406..36481)
                     /locus_tag="RH222_000709"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36446..36448),aa:Gly,seq:gcc)
     gene            complement(36518..36593)
                     /locus_tag="RH222_000710"
     tRNA            complement(36518..36593)
                     /locus_tag="RH222_000710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36558..36560),aa:Gly,seq:gcc)
     gene            complement(36804..37349)
                     /gene="orn"
                     /locus_tag="RH222_000711"
     CDS             complement(36804..37349)
                     /gene="orn"
                     /locus_tag="RH222_000711"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV3795772.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            37444..38496
                     /gene="rsgA"
                     /locus_tag="RH222_000712"
     CDS             37444..38496
                     /gene="rsgA"
                     /locus_tag="RH222_000712"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV3795773.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            38593..39561
                     /gene="psd"
                     /locus_tag="RH222_000713"
     CDS             38593..39561
                     /gene="psd"
                     /locus_tag="RH222_000713"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV3795774.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            39583..42906
                     /gene="mscM"
                     /locus_tag="RH222_000714"
     CDS             39583..42906
                     /gene="mscM"
                     /locus_tag="RH222_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV3795775.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(43057..44559)
                     /gene="yjeM"
                     /locus_tag="RH222_000715"
     CDS             complement(43057..44559)
                     /gene="yjeM"
                     /locus_tag="RH222_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV3795776.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(44778..45755)
                     /gene="epmA"
                     /locus_tag="RH222_000716"
     CDS             complement(44778..45755)
                     /gene="epmA"
                     /locus_tag="RH222_000716"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV3795777.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            46080..47888
                     /gene="frdA"
                     /locus_tag="RH222_000717"
     CDS             46080..47888
                     /gene="frdA"
                     /locus_tag="RH222_000717"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV3795778.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            47881..48615
                     /gene="frdB"
                     /locus_tag="RH222_000718"
     CDS             47881..48615
                     /gene="frdB"
                     /locus_tag="RH222_000718"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV3795779.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            48626..49021
                     /gene="frdC"
                     /locus_tag="RH222_000719"
     CDS             48626..49021
                     /gene="frdC"
                     /locus_tag="RH222_000719"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV3795780.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            49032..49391
                     /gene="frdD"
                     /locus_tag="RH222_000720"
     CDS             49032..49391
                     /gene="frdD"
                     /locus_tag="RH222_000720"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV3795781.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            49454..50587
                     /locus_tag="RH222_000721"
     CDS             49454..50587
                     /locus_tag="RH222_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV3795782.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            50676..51209
                     /gene="blc"
                     /locus_tag="RH222_000722"
     CDS             50676..51209
                     /gene="blc"
                     /locus_tag="RH222_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV3795783.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(51206..51523)
                     /gene="sugE"
                     /locus_tag="RH222_000723"
     CDS             complement(51206..51523)
                     /gene="sugE"
                     /locus_tag="RH222_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3795784.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(51698..51844)
                     /gene="ecnB"
                     /locus_tag="RH222_000724"
     CDS             complement(51698..51844)
                     /gene="ecnB"
                     /locus_tag="RH222_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV3795785.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(51955..52080)
                     /gene="ecnA"
                     /locus_tag="RH222_000725"
     CDS             complement(51955..52080)
                     /gene="ecnA"
                     /locus_tag="RH222_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV3795786.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(52132..52698)
                     /gene="efp"
                     /locus_tag="RH222_000726"
     CDS             complement(52132..52698)
                     /gene="efp"
                     /locus_tag="RH222_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV3795787.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            52740..53768
                     /gene="epmB"
                     /locus_tag="RH222_000727"
     CDS             52740..53768
                     /gene="epmB"
                     /locus_tag="RH222_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV3795788.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT        13150 a        14559 c        13455 g        12905 t
ORIGIN      
        1 ttaaccgaac ttaaacaacc actctaaaga taacaaacaa caaccaacca tagtaggaat
       61 ggagaaatta tggattactg gttaccgatt attgtgttat ttggtgcctt cttttttatg
      121 ttggcattag gtgtacctat tgtttatgcc attggtttat cgacactggc atcaatttcc
      181 acacaactcg atttcaactc agcattgagt gtggtttccc aaaagttagc ctccgggtta
      241 gatagtttta cgttgttggc aatcccattt tttatcttgt ctggcaacat tatgaaccat
      301 ggaggaatag cgcgacgatt aatcaacttt gcccgtattc tcggcggtcg tctgccaggc
      361 tcacttgccc actgtaatat tctcgccaat atgctgttcg gggcaatttc tggttcagcc
      421 gtggcttcgg cggctgcgat gggcggcgtc atgcatccgc aacaggttaa agagggttac
      481 gacccggcat tcagtactgc ggttaacgtt gcctctgccc cgacgggttt gcttattcca
      541 ccaagtaata cgttaatcgt ctattcactg gtcagtggtg gtacttctat tgccgcactg
      601 ttcctggcgg gttatgttcc gggaattttg ctgggtctgg cactgatggt aatagccggt
      661 attatcgcgg tacgtcgcgg ttatccaaag ccagaacgcc cgactttacg ccaggctggc
      721 gttgcgatat ggatggctat ccccagtatt ttcctgatta ttttaattat ggggggtgta
      781 ttaagtggta tttttacccc gacagaggcc tccgcaattg cagtaattta tacccttttc
      841 cttgcactgg ttctttatcg tgaaatctca gttaaagatt tacctaaaat atttctggaa
      901 tccgttatta ccactgcaat tgtcttgtta ttaattggtt cttcgatggg tatgtcatgg
      961 gcaatgtcaa acgctgacgt tcccttcctg attctcgatt tgcttaacac tatttctgac
     1021 aacccaataa taatcctgct tattatcaat attatcctgt tgattatcgg cacttttatg
     1081 gatatgacac cggcggtact gatttttacg ccgatatttt taccggttgt caccgaactg
     1141 ggcatggacc cgattcactt tggtattgtc atggtactga acatgtgtat cggtatttgt
     1201 actccgcctg taggcagtgt gttatttgtc ggttgtagtg tatctaaatt accgattaat
     1261 aaaattatca aacctatgct gccattctat gccgtgatgg tattggtgct ggcaatggtg
     1321 acctatattc cgcaaatcag tatggcattg ccaagagcac tgggctactg atgcgcattc
     1381 actggcggca cctgccgcca tatttattag aaaaggaaca tctatgaaaa taaaagtatc
     1441 cgctggcatt atcggtgctg ttcttatgtt atccgcaagc cagtcctggg cagtgacatt
     1501 aaaactgagt cataatcagg ataagtctca tcctgttcat aaagcgatgg agttctttgc
     1561 gaaaaagagc aaagagtact ctaacggtga tattactatt cgtatttatc caaatggaac
     1621 attgggtact caacgagaaa caatggagct gattcgttct ggcgctattc ccctggtaaa
     1681 aaccaatgcg gcagaaatgg aagcatttga aaattcctat aaattattta gcctgcctta
     1741 tttgttccgc gatcgtgatc attattatca ggtcatgcag ggcgatatcg ggagaaaaat
     1801 cctcgactca acgaaaagca aaggttattt cgggctgact ttttatgatg gaggcgcccg
     1861 cagtttctat ggcaataaac cagtactgaa accagacgat ctgaaaggca tgaaagtccg
     1921 tgtccagcca agccctggcg cagttgaaat gatcaaagtc atgggcggta acccgacgcc
     1981 actggattac ggcgagttgt atacagcctt acagcagggt gtggtcgata tggcagaaaa
     2041 cagcgtgatg gcgctgacta ccatgcgtca cggtgaagtg gcaaaatcct tcagccttga
     2101 cgaacacact atggttcccg atgtggttct gatgagcaat gctgcgtttg ataaacttag
     2161 cccggaaaat caggcagtta tattaaaagc agctaaagaa tcaatgagct acatgaaaga
     2221 cttgtggagc gaggaagaga aacaagaatt tgcaaaactg gataaaatgg gcgtgaaagt
     2281 ctaccaggta gataaagctc cgtttatcga gaaagtacag ccaatgtacg caaacttcgc
     2341 taaggacaac ccagcccttg ccccaatgct ggctgatatt caggcagcta agtaattatc
     2401 ttcgtagggc tgcttattgt ggcccttatc gatatctgca agtgctcgct acaaaagcaa
     2461 aacgccgacc aatggtcggc gtttttacgt ctcgttgaat aacgaattat tcagctacta
     2521 cgtttacgat cactttcgcg aatacttcgc tgtgaacctg gaagctcact tcgtgttcgc
     2581 cagtggtacg cagaacgccg ttcggcagac gaacttcgct cttagccact tcaacgccag
     2641 ctgcagttac agcgtcagcg atgtcgcgag taccgatgga accgaacagt ttaccttcgt
     2701 cgccagcttt agacgcgatg gtaacagttt ccagtgcatt gattttctca gcgcgagcat
     2761 tagcagctgc cagaacttca gccagtttag cttccagttc agcgcgacgt gcttcgaaga
     2821 attcaatgtt tttcttggta gctggaacag ctttaccctg cggtaccagg aagttacgag
     2881 catagcccgc tttaacgttt acctgatcac ccaggctacc caggtttgct actttatcaa
     2941 gcagaataac ttgcattacc ttatcctctc aaagtcgtat taatggaccg tgaccgatta
     3001 ctgatggcga tcagtgtacg gcagcaggga caggtagcga gcgcgtttga tagcgcgagc
     3061 cagctgacgc tggtattttg cacgggtacc ggtgatacgg cttgggacaa tcttaccgct
     3121 ttcggtgatg tagtttttca gcgtagcgat atctttatag tcgatctctt gaacgccttc
     3181 cgcggtgaaa cggcagaact tgcgacgacg gaaataacgt gccatatggc tagtctccag
     3241 aatctatcaa ttcaatctgc tcggcatgca aaaccatttt gctcagtccg ttctttgcct
     3301 tgtggcatga aatgaacccc tgaacggtta tgcgactgcc gaccgttata ctgtgagtaa
     3361 tgacctggtt ttcgtgtccg ctaacaataa cgggcatttg acaccatgcc tgccggtgaa
     3421 agccggcttc ctcctgcaca gaacgatgct caagcacgaa ctggcagtga ggaattcctg
     3481 atggactgac ctttcgaagg ggagtcctgc acacggtgcc ggacaacacc agacggttgg
     3541 tcatcagaaa ttactcttca gaatccccag cttcagcatc atcagcggtt tcgtttgcga
     3601 aatcatcgcg acgctcacgg cgctcgtctt tcgctttaac catcggagat gcttcggtaa
     3661 cagcgtgctt ggtacgcata accatgctgc ggataacggc atcgttgaag cggaaggtag
     3721 tttccagctc atcgatcact tcctgcggag cttcaacgtt catcagaacg tagtgtgctt
     3781 tgtgcagttt gttgatcggg taagccagct gacggcggcc ccagtcttcc agacggtgga
     3841 tcttgccttc tgcaccagtg atggcagcag tgtagcgctc gatcatgccc ggaacctgtt
     3901 cgctctggtc aggatggacc ataaaaacga tttcgtaatg acgcatcgaa ttgctcctta
     3961 cggattattc agcctcctgt ctgggtcagc cgaatcccgg ggaggcaagg aacgtgttaa
     4021 aggtcggctg aaaaatgacg cgttatagta cttgtatgcc ccgctaaact caaggccagc
     4081 cgacaaataa atctcatctt tcgctcaaaa gaactaactc cctgatttga agttcatcac
     4141 attccgtcac agtgtttttt cgaacaactg catcagaaat ggtcaccatc aacttcttta
     4201 tcattgatta aaattaaatc agaagatcat cacagaggag agcacacaaa gtcagccgtc
     4261 gaggaggtat ctatgttcag tcgtgtttta gcccttctgg ctgtgctttt gctaagtgca
     4321 aatacatggg cagccattga aatcaataac caccaggcca gaaatatgga cgatgtgcaa
     4381 agcttaggcg tgatttatat caatcataat ttcgccactg aaagtgaagc acgtcaggca
     4441 ttaaatgaag aaacagatgc gcagggcgca acgtactacc acgtaattct gatgcgggaa
     4501 ccggggagta acggcaatat gcacgccagc gcggatattt atcgctagca ccaggtataa
     4561 ccaacgaaac attgccatag tttgctttgc ccccttcgca gggggctttt tttgaaaaac
     4621 ggctgcggaa ttagaccagt tatctcccga agaaggaaat ttccgcagcg cgtgttttac
     4681 ttctgcccgt aataagcgtt aggaccatgc ttacgcatga agtgtttatt catcaggaag
     4741 ctgtcgatgt gattgagttg tggattaata ctgcgcgcaa tccacgccat tttcgccact
     4801 tcttccatca ctaccgcgtt atgcaccgcg tcgtgggcat ctttccccca ggcgaacggt
     4861 ccgtgctgat acaccacaat tcccggcgta tgcagcggct cggcgttgcc cagcgtttcg
     4921 ataatcactt tgccagtgtt cagttcatac tcaccctgca cctcttcttc gcttaatcca
     4981 cgcgtacacg ggatgtcacc aaagaagtag tcggcgtgcg tggtgcctaa cgccgggatc
     5041 gccagccccg cctgcgccca tgcggtggca tgagtggaat gggtatggac aatgccgccg
     5101 agcgacgggt aacgacggta gagttcaaga tgcgtcgcgg tgtcagaaga cgggcgatac
     5161 ttcccttcta ccaccttgcc gctcatatcg accaccacca tatcgtctgc tttcatggtt
     5221 tcgtaggcaa cgccgctggg cttgatcacc accagcccgc gttcgcggtc gatagcgctg
     5281 acgttgcccc aggtaaaggt caccagccca tagcgcggca gatccatgtt ggcttcaaat
     5341 acctgctgtt ttagcttttg cattatgccg cctccaccat gcccgctttc gccatgcgcg
     5401 ctttcaccca atcacgcgct ttcgccactt ctgccgccgg gtcttccgcc gtttcgctcc
     5461 acatctcaat caggtacggc ccgcaatagc cactctgttt gagcgtttcg aaacaacgtt
     5521 cgaaatccac tacaccttcg ccaaacggca cgtttttgaa gacgccaggt ttggtgtctt
     5581 tcacatgtac cgcgacgata tgcccgattc cggcctgcaa ctccatctgc acatcgttgt
     5641 cccacgccga caggttgccg atatccgggt agagctggaa ccacgggttg ttgagatagt
     5701 gcgcgtaacc cagcgccttg ctgatggagt tcatcaacgg ataatccatg atctccatcg
     5761 ccagcgtcac ctgcgcgcgg ctcgccatct caacgctctc tttcaggccg tcacggaaac
     5821 gacgacgcgt ttcgttattg gcttcctgat agtaaacgtc atagcccgcc agctggatca
     5881 cgcgaatgcc gacgtcctgg gcgaactgga tagctttacg cataatctcc agcccctgcg
     5941 cccgcaccgc gtcatcttca ctgcccagcg ggaaacgacg atgagcagaa aggcacatgg
     6001 acggcacgcg cacgccggtt tcaacaatcg cattcaccag cgccagacgc tgctcgcggc
     6061 tccagtcgag gcgcgacagg cgctcgtccg tttcatctac cgacatctcg acaaaatcga
     6121 agcccaacgt ttttgccagt tgcaggcgtt ccagccagca ctccccggcg gggagcgctt
     6181 tttcatagat gccaagcggg atttgtttgg acaacatatc cgctccttag ccccacagtt
     6241 cggcgatgga acgtttgaac tgacgtgcag cttcaaccgg agaagcggca tcacggatgc
     6301 tgcgaccagc gataaacaca tgaatcggaa tacctttgaa cagcggcaga tcttccagcg
     6361 ccaggccacc ggtgacggtg actttgaagc ccatatcgga aagacgtttg atcgcggtaa
     6421 tgtccgcttc gccccacgct acgcctgcgg cctgcgcgtc acggctgcgg tgataaacca
     6481 cctgctgaat gcctgcgtca cgccactgct gcgcctgttc ccaggtccag tagccggtca
     6541 attcgatctg cacgtcgccg ttaaactctt ttgctacgtc cagcgcgcct ttggcggtgt
     6601 tgatatccgc acagcaaatt accgtcaccc agtcagcgtt ggcttcgaag cacatacgcg
     6661 aaaggatttt gcccgcatca gcaattttag cgtctgccag tacgattttg tgcgggtaga
     6721 gcgctttcag gtcacgaacc gcacgcacgc cttcgcccac gcacagaatg gtacccactt
     6781 cgataatgtc gacttcttcg gcaatcaggc gagtggtttc gtaggcgcta tccatagtct
     6841 ggttgtccag cgcgacttgc aacatcggta atgacatctt caattccttc ttaagctgcc
     6901 gcgttggtgc ggtcaatgag atccagtact tcctgctcgg tacggcaggc acgtaaacgg
     6961 tcgaaatttt cttcatcttc aaacaggttg acgatctgca tgatgcccac ttcctggtga
     7021 gtgttggcat cgaccgccgc catggtgatg agtatatcca ccgggtcatt atcttcgtgg
     7081 ttgaactcca gcggcttttt cagcgtcacc agcgcaaaac cggttttctt aacaccctct
     7141 tccgggcgac cgtgcggcat tgccaggcct ggtgcgagca caaagtacgg accgtgctgt
     7201 tcaaccgcat ccagaatcgc ctggtagtaa cgcggctcga caacatccgc cgccaccagc
     7261 aggtcaacgc cgatttttac tgcgtcctgc catgtctcag cttcagcctg caggcggatg
     7321 gatttattct ccgccagcga atcacgtaat ttcatggtgc gtccttactt cacatcctgc
     7381 gggaaatgct ctttgatcac ttccagcagt ttcgggccaa agtcagcagg agagagcatg
     7441 ttgcgcacgc caaccacgta tttgttgccg gtcacggtga tttcgcccgc aatgtgcgta
     7501 gaagcgatga tgatatccgc gccgctcaac tcgcttttgt attcgccaac cgcgcagctg
     7561 tttaccgtgt ggtcaatgtt tgattgggtt aaaaactggt ccactttcat cttcatgatc
     7621 atggaactgc cttgtccgtt gccacacaca gccagaatac gtacggtcat aatcaaaact
     7681 ccttattaag cagactgttc tgccagttgt ttttctgcat cttcttctgc gcgcagtgcg
     7741 cggccagcga agaacatata agccagtgca attacaatga tgacggccat aaaggcgata
     7801 ccgatggaga agaagccctg catcatcggc ggtgccagaa tcgaccagtc cgccataccc
     7861 atccaggcac tcataccggt aagtttcacc gcccagacgc aaccaaagat ttcgatcatc
     7921 cccatcacca gacaaatctt cagcgccgca cgccagccgc cgaagtggtt agcgaacacg
     7981 ccgatggtgg cgttggagaa gaacatcggg ataaagccag gaataatcag gattgaggaa
     8041 ccgcaggcga ccaggatgcc aaccgcaatc agctgaccga tggtgcccca cataaacccc
     8101 cagaccacgg cgttcggcgc gaagctatag atagccgcac agtcaatcgc cagaaccgca
     8161 cctgggatca ggcgctggga aatgccgtta aatgcttcag agagttccgc cacaaacatg
     8221 cgcacaccct gagtgatgat gaagatcgcc accgcgaagg agaaaccagt ttgcaggatg
     8281 tacaccgtcc agttcacttt gcctgccatc gcctgcacgg tgtcgatacc gaaggagagc
     8341 agaatggcac caaagaagat ggtcatcaca atcgccgtgg agacgatgtt gtcgtggaaa
     8401 atattcagcc agcctggcag tttgaggtct tcaacgcttt cctctttttt gccgaggaac
     8461 ggcgcaactt tataggcaat ccatgatgca aactgttgct ggtgaccgat ggagaaaccg
     8521 cagccgtccg tcacttcctg agtcggcttg tacattatgt tggaggtaat gccccagtag
     8581 agcgaaacca gaatcgccgt gcagataatg gtcgtccaca tggagtagcc gaaaataaac
     8641 aacgtaacgg caatcagccc cgcctgctgg aacatgatat gaccagtcag catgatggta
     8701 cgaataccgg taatgcgacg cagcagcacg taacagatgt tcaacgccag cgctaacaac
     8761 accgcgtaac ctacccagct ataggcatcg cccatgcggt cgatggttgc catcatcgac
     8821 gcgtaagtat cggaaattgc gccgttaatg ccgtagactt cggacatttt cgccaccacc
     8881 ggtttgaagg tgctggtgag gatgccggac cctgcctgca acaacatgaa accaattatg
     8941 gttttaatcg tgcctttaat aataacgctg acacttttgc gcagtaggat gtagcccaga
     9001 caggtcacaa tacccagcaa caacggggca ttggtcatga cctggttaaa aaacacggta
     9061 aagatgttgt agaggatctc cataacgatc tcctgaagaa gaggtaaccg ggtattccac
     9121 acacccggaa tgttgtgcac tcactctaat tttcaaaagt aatcacaaca agattattta
     9181 tgattaaatg tgacgcgacc cgcaaataat tccacatgga tagaatggga ttcacacaac
     9241 gaaacaacta ttcatttaaa ttcatttagt taattgaatt acacattcca ttaaatcttt
     9301 ccacactcaa aatcaacaac cacttccctg gcaaaaagtc aaaaaatggt cattgccttc
     9361 ctgaaaaaat agtgtcagga ttaatcaaaa ccaatcacat attgattcga attgaactat
     9421 catgaaggta aatggcgatg agtaaagtga aaagtatcac ccgagaatcc tggatcctga
     9481 gcactttccc ggagtggggt agctggctga atgaagaaat tgaacaagag caggtcgccc
     9541 ctggcacatt tgcgatgtgg tggctaggct gcaccgggat ctggttgaaa tcagaaggtg
     9601 gcaccaacgt ttgcgttgat ttctggtgcg gcactggcaa acaaagtcac ggtaacccgt
     9661 taatgaaaca gggtcaccag atgcagcgca tggctggcgt gaaaaaactg cagccaaacc
     9721 tgcgcaccac cccgtttgtt cttgatccgt ttgcgattcg ccagatcgac gcggtactgg
     9781 cgactcacga tcacaacgat catatcgacg ttaacgttgc tgctgccgtg atgcagaact
     9841 gtgctgatga cgtaccgttc attggaccga aaacctgtgt ggatttgtgg attggctggg
     9901 gcgtaccgaa agagcgttgc atcgtggtca aaccgggcga tgtagtaaaa gtgaaagaca
     9961 ttgaaattca tgcgcttgac gctttcgacc gtactgcact gatcaccctg cctgccgatc
    10021 aaaaagcggc tggcgtactg ccagatggca tggacgatcg cgcagtaaac tacctgttca
    10081 aaacccctgg cggttcccta tatcacagtg gcgattccca ctactctaac tattatgcga
    10141 agcacggtaa cgaacatcag atcgatgtgg cgttaggatc gtatggtgag aacccgcgcg
    10201 gtattaccga caaaatgacc agcgccgata tgctgcgtat gggtgaagcc ctgaatgcga
    10261 aagtggttat cccgttccac cacgacatct ggtcaaactt ccaggccgat ccgcaagaga
    10321 tccgcgtgct gtgggagatg aaaaaagatc gtctgaagta tggcttcaag ccgtttatct
    10381 ggcaggttgg cggcaaattt acctggccgc tggataaaga caacttcgag taccactatc
    10441 cgcgcggttt cgatgattgc ttcactattg aaccggatct gccgttcaag tcattcctgt
    10501 aatctgtcgt ttataccgga tagcgtggag aagttcgcgc tatccggcgt agtatttcct
    10561 ggctatttca aatatcatct aaaaaaatca aattttatcg gaatagctca tgactgaagc
    10621 acaaagacat caaatcctcc tggaaatgct cgcacaattg ggctttgtga ccgttgagaa
    10681 agtcgttgag cgtctgggaa tttcgcctgc cactgcgcga cgcgatatca ataaacttga
    10741 cgaaagcggc aaactgaaaa aagtgcgcaa tggcgcagaa gctattaccc aacagcgccc
    10801 gcgctggacg ccgatgaatc tgcatcaggc gcagaatcac gatgaaaaag tacgtatcgc
    10861 taaagcggcc tcgcagctgg ttaatccggg cgaaagcgta gtcatcaact gcggctccac
    10921 cgcgtttctg cttgggcggg aaatgtgtgg caagccagtg caaatcatca ctaattatct
    10981 accgctggca aattacctga tcgatcaaga acatgacagc gtgatcatta tgggcggaca
    11041 gtacaacaaa agtcagtcca tcactttaag cccgcagggc agcgaaaaca gtctctatgc
    11101 cgggcactgg atgtttacca gcggcaaagg actgacagca gaagggttgt ataaaaccga
    11161 tatgctgacg gcaatggcag agcagaagat gctgagcgtg gtagggaaac tggtggtact
    11221 ggttgatagc agtaagattg gcgaacgcgc gggaatgctt tttagccgtg ccgatcaaat
    11281 tgatatgctg atcaccggca aaaatgctaa cccggaaatc ctgcaacaac tggaagcgca
    11341 aggggtcagc attctgcgtg tttaaagatg ctggcggaaa aatgtcactg cagcatctaa
    11401 cgcctcaggc gtaatgcggt gacgcacgcc tggctgccat gaacaggtta gcagtttatc
    11461 ccgtcccgtc tcgcttaagg cctgctgcaa acgtagtgat tcgtcggcag gcacaacgtc
    11521 atcgtcgagg ccatgccaca gcagtagagg tctgtcacta agttgttcca ggtggtttgt
    11581 cgcttcccac tctgccagcg gcgcgacaat gttattgaat tcattctgct gtgctgtcgt
    11641 ttcaggtatc agcggtggaa acagtgaacg ggcgagtgat gtaaaatagc ccgctcccat
    11701 catgctggcg gtacatctca ccgtggggtg gcgagcggta atccccagtg ccgtcatcgc
    11761 gcccatcgac gcaccaccga ctgccagacg gtcatcaagc agccagtttt cttcggctat
    11821 tgccgcacgt aaagtagtga attcctgcat actttgtagc aagatttgcc agaattgatt
    11881 taaccgccgc gctgcgtcac cactaaaacg gctaccgtga tcgggcgcat ccggcatgat
    11941 cacccgcaaa ccagcctgcg ccagcgcaac ggcaaaatag ctatacacca gactggatga
    12001 agtaaagccg tgataaaaaa ttacgcacgg taacggggta tctttttgcc cgacaggata
    12061 agcatgaaga acgggaatat ctgccagctc gcgtgattct atttcaatca tctgtctctc
    12121 ctttttgtct ggtatgacat gaagaaatcc ttagcacaag tttctgctgg cggtattagt
    12181 tcataatgtt gagatgtggg ttacgctttc gtcacgtttt cattcgaaaa tcgcgtacca
    12241 ggtaacaatt cgggaacatt cccccaaagt taaattaata aggcactaca ctatgtttag
    12301 caggaaacgt aatagcgtta tttaccggtt tgccagttta ttattggtgt tgatgttaag
    12361 tgcctgtagc gcactgcaag gtacgccaca gccagcaccg ccagtaacgg accatccgca
    12421 agagattcgc cgcgaccaga cgcaagggtt acagcgaata ggcagcgtaa gcacgatggt
    12481 tcggggttct ccggatgacg cattagcaga aatcagagcg aaagctgtcg ctgcaaaagc
    12541 tgattattac gttgtcgtca tggtggacga aaccatcgtg acaggacagt ggtattcaca
    12601 agccattttg taccgtaaat aaatcagaca acttacatag tcagctttac attgctttgc
    12661 gtccctatgc gtttacctgt gcacaataaa ttaccggctg gaagcctggg cgtttattac
    12721 cgcgatggaa tgccctgcaa catgtgggga aacgaaaaat ggagctgacg atgaaacaat
    12781 tacttgcctc accctcgctg caattagtga cttatcctgc gagcgccacg gcacagtctg
    12841 cagaattcgc cagtgctgac tgcgtgacgg gcctgaatga aataggtcag atctcagtta
    12901 gcaatatttc aggggatccg caggacgtgg aacgtatcgt ggcgttaaag gccgatgaac
    12961 aaggtgcgtc atggtaccgc attattacca tgtacgaaga ccagcagcct gataactggc
    13021 gcgtacaggc gatactgtat gcatgagtgt catctacagc aaatgaacaa tgccggatgc
    13081 ggcgcgagcg ccttgtccgg cctacaattt tcacatattt catttagtta atcgaaacca
    13141 gcgtcgcatc agtcgtatac gcttacacac acactccccc cgtcgcccgc agcaataaat
    13201 cattctggat ctgctctgac aaccgtacgc cgccgcgcgt atctaacatc acctgacacc
    13261 acgcctgcgc cattggcgga gaagcatatt tcaacatttg cgcaccgcag cccagcagga
    13321 atagctgatg agtaatctct cgcctcagtt cttcagctgg cttacgcagc tgctgctgta
    13381 aacgacgaac cgcgcgatca aaatagcgat cctgcccttt cacttccaca aacgcttccg
    13441 acaataagtc gtatacgccc gcttgcttat tgagaacgcg cagcacatcc aggcacataa
    13501 tattgccgga accttcccaa atactgttta ccggcatctc ccggtaaagc cgcggcagct
    13561 cgctctcctc gcaataacca atgccgccca gcacctccat cgcttcggcc acaaacggca
    13621 tacctctttt gcagatcaca aattttgccg caggcgtaaa taaacgcgcc cacagggctt
    13681 ctttggcatc ggcacgccgg tcccacgctc gcgcaagacg aaacagcaac gccgtttgcc
    13741 cttcaagctg aagtgccatg cgacttaaga catgacgcat aaggggctgt tggatcaatg
    13801 gattaccaaa aacatggcgt tgatgtgcat gataaatcgc cagagaaaat gcacggcgca
    13861 tcatggcatg gctacccagg gcgcaatcaa aacgcgtcat cccacccatt ttcaggatca
    13921 gacgaattcc ttccccttcc tgccccaaca accagccaat ggcatcctga aactccactt
    13981 cgcagctggc gttagagcga ttacccagct tatctttcag ccgctcgagg cgaatcgcgt
    14041 tgcgttgccc gtcaggcaaa aagcgcggca caaaaaagca ggacaaaccg cccgtagtct
    14101 gcgccagcac cagatgcgca tcgctttgcg gcaccgagaa aaaccattta tgccccacca
    14161 gccgataaga gccatcttcc aggcgctctg cgcgggtggt gttgctcatg acatcggaac
    14221 cgccctgctt ttccgtcatt cccatgccaa tcaacaaacc gcgtttttgc ccacctggca
    14281 ataagtgaga atcgtagcga tcgctcagca gcggcgtggt ccagtcctga aacggcgcgg
    14341 gtaacatctg taacagcaat ggcgtggcgg caaaggtcat ggttatcgga cataacgacc
    14401 ctgcctcaac ctgtgcgtgc aacataaaac gcgccgcgcg cgccacaaat gcgccggagc
    14461 gagcgtcttc ttcccaggca agattgtgca cccgattggt acatagcgcc tgcatcagca
    14521 ggtgccaggc ggggtgaaaa cgcacatcgt ccagacggcg tccttgcgca tcgtagcgca
    14581 ataattcagg cggattcaca ttcgccagcc gccccagttc aagggattca gccgttccta
    14641 actgctgacc aatactggct agaaaatcgc tatcccagcc agcaccttca cgcgttaccg
    14701 cttcgcagag cgcgccatca gacaggtata agttgctgtt atttaatggt ataggttgat
    14761 taaaaacggt gtgagtttgc cagtgcactg tgtctccctc catcaatggc agtcaccatt
    14821 agtatggtca ctgccatgga ttcatgacag aaagagattg ctaaaacatt cttatccagc
    14881 aatgtaatgc ggaacgaaac gtgaggtgtc tttggtgatc agtgtgttat cttcacggat
    14941 ccccattccg cacgcttcat catcgacaat ccagctaccg atgagtgtgt agctatcgcc
    15001 aaatcgcggc agaggttgaa acgcctggta gatcatcggt tcatcggcgt aatcaccatc
    15061 agcgtggtca acgacgttat tctgaccgtc gaaaatggtg acgtttccgc cttcgcgcga
    15121 gtagatcggt ttacgcacat agctttcgcc agcggcaatc tgcgattttt caccctcaaa
    15181 ccaggatgca agaaggttag gatgaccagg gaagaagcgc caaagcagcg gcattagccc
    15241 tttattactc aagatacttt tccataacgg ctccacccat tgctcgcgac gcttgcacag
    15301 cagcggaccg ttatcgtcac gcatcatcca ctccagcgga tacagcttaa atgcacgctg
    15361 gatgacatta tcatcaagat cggtcagtac accgccgacg cccaaaccga gatcttcaat
    15421 gtagataaac cgcgactcct gccctgcctg ctgggcgcag tcctgcaagt acagcacggt
    15481 agtcctgtct tcatcggtgt cctgacagca gcagaaataa aacggctccc ggctataaag
    15541 ctcgctaaag cgtgaaatca ggcgttcctg aatggcattg tactgatcgg catcacgcgg
    15601 aataatgccg ctgcgccggg catcctccag ccacaaccac tggaaataag ccgactcata
    15661 caacgaggtc ggcgtatcgg cgttgtactc cagcagcttc accggcgcat tgccacacca
    15721 ggcaaaatcc atccggccat acagcgaagg atcgcgtgcg cgccagcttt cggcgatcac
    15781 atcccagtac aacggcggga tcgccagttg cgtcaggatc tcttcgtctt taaccgcgcg
    15841 atccaccacc tcaaggcaca tctgatgcag ttccgcagtc ggtttttcga tctgctcttc
    15901 aatctggcgc agagtaaaac ggtaagcccg actctcgtcc caatagattt cattgtcgat
    15961 gatatgaaag tcgaaaccgt tgttagcggc gatctggtcc agatcccgtc gcacaggaac
    16021 gttgtgtctc agcatgatta gcctccccaa tgcccacggg cgctggaaga acgaccatag
    16081 ccgccgcgcg aaacggttga cgcttttttg gtggtaaagc cgcctgaaga gtaagactct
    16141 tttttgccag aaccggagcg ccaggagtaa tcaccggaag tgttgcgcca gacggggcgg
    16201 gaagcaaatg aggatccgcc gctgttataa acaaactgct catcgcgatc tttacgaata
    16261 acccggctta acaaaaatcc agatattacc gggatccaac tctgttcaac gttgtcgtag
    16321 taacaatttt catacttaga ctgacagttc tgctgagtca tattcttcgg aacatcggca
    16381 taaaacgccg ctttggcgtt attccagcca cgcgcgcaaa tgtcggcatt attaccgtca
    16441 tcaatacaat cctgcactgt cgagtaaaac gttccgtcgc cgtcgttatc gacatcgcta
    16501 ctttcactcc atcctttgac tattaaaaaa acggcaccgc ccattagcac caacgttaag
    16561 tatttttgtg cgtagcgatt gcggcgcggc tcaaaaggat tatgcggtct tccaatgcga
    16621 ctgatcgctc cgtgaccaat tttactgtta tttcttgatt tgcgttttct ggccatgatc
    16681 gtccattacc atgtcataca agctgcgtta aaaataccgc cagccagcgc ggcggttccc
    16741 ataaacattc ctgctgcggt attgtgatta ataatttttt cgcttaatgc gggcatataa
    16801 agtctgacac cagcaaaaac gagtaactga atcaccagtg caatcccgcc ccaggcgaaa
    16861 tagtctggaa tactcaccgc attgattgcc gcactggata atgggataac gtaacctaac
    16921 aatgtaccac tgaacgccag tgaagccgcg gtattattgt ttttgattaa ctgccattcg
    16981 ttgtgcggtg taattttaga atagataaaa agaaaaataa tcaccatagc cacgccaata
    17041 aaaaaatagg cgctaaaggc aagaagtgaa tccagtatat gcattgtaaa cgtccttgta
    17101 attaaccaat aatatgaagt gatgtcagcg ggatatcgac acccaacgcg cgcgaaaata
    17161 accactctgg ctcgccgaga tcgttaaaag attcctcacc atttaacagc aaatattcgt
    17221 aagtatcttc ggttacttgg cgttggtagc ccatggtaaa attatggacc tcccatttgg
    17281 catggttttg attttctact ttttccagca tgtagacggg ttcgatattt cccggttctt
    17341 cgctgttaaa aaatcgctgc cagcgttttt cctgccagtt taagatcatt gcccccatca
    17401 ctttggcgtt gatcgcctcg cgccagtgac tctctttact gataccgtag ctctcttcat
    17461 agacaaagag cttgatatca tcaatgtcat caatatcttc gccgccggtg gtattgattt
    17521 gcagaaattc atcgcctgaa gtgtagtagc ggaaaatctg actaccgccg cccagatcga
    17581 tgtggctgac agcggctacc gtaaattcct cacccggcag cgcaatcagc aattcatctt
    17641 ccagcaaacg aaacgctaac gtgtcgagcg taaagccact attgagatga agtcccagcg
    17701 gaccgcgagc gatagcaggc ttattatcct tgccaaacag acgctggaaa aaaccagaca
    17761 tattttatcc ttaaaatagt tgccagcctt ttagcgaggc tggcaaaaaa attactcgcc
    17821 ctgttggcgt tgcagtcttg ctaatacatc ctgggcgcta cttttattgc taccgccaat
    17881 tccggcttcc gccagctttt catcaagatc gcgaccgtct gcgacttttt ccaactgtgc
    17941 agcagcatcc aggcgagcct gacgttcggc ctgacgcgtt tgcaggcgtt ttaaggattc
    18001 tgccgctgtc gaaacgctgg aagatgcgcc aacggtagag gttgttacag cctgttgtgc
    18061 acgctgcatg gcttcagtgg ctttaacgac ttccatttgc tgctcaaact gagcgatacg
    18121 ctgcgctgtc gctgtaactg ctttttccac gccatcacgg gagacttcca gattcgacaa
    18181 cacttgctct tcagcggtaa tgagattctc aaggcgcgcg atttcttcag caacttcgtt
    18241 aatcaacgac ggattaacgt tcttgcttaa cgcttccagc gcacgggctt ccagactggc
    18301 tttgcgctca cgtaaatctt tcagcttatc gtgacttaat ttcacccgcg ccagcagatc
    18361 aacgcgagat tttccggctt tatcgagttc agctttagcg tcacgaatat gctgttccag
    18421 catgcgcacg ccctgggttt cttcaatgga ttcttccgcc tgggagataa acgatttccc
    18481 cagcgtaaat aaacttttta aaattcccat aaatacccgt ccttgtaatt agtgtgaata
    18541 ttcttctgta atttcagcca gatccaatgc gttatctacc agcgaggtta tctcaagcag
    18601 gatatcttca agagaagatt taagcgataa cgccccgaaa acaatgtaat actcttcctg
    18661 ttgcacgctg gagattccta ccgatgacaa cggcatcatc ttctgatttc ttaataagaa
    18721 ggtattaaat tcatctggat tgctgatact acttaccgga caaataaagg tttcgataat
    18781 catttggcgg gaagtaaaaa gaatattgat ttgcaaatcg ccataatcat tcattttaat
    18841 aattaatgcg ctctcgctat ttgtcacatc aatattttgt tcaggtacag tttgcagcgc
    18901 cgtcgccagc gccaacggat tccatgtcat atattattcc atatagattt agtttaaata
    18961 ttaataaaat gaatatttgc agtacgtaat tatcttacca gctatagaca aaaaaaaacc
    19021 atccaaatct ggatggcttt tcataattca gacgaactag ctgcgctgac gaaccgcttc
    19081 aaataagcaa attccggttg caaccgaaac gttcagggaa gaaacacttc ctgccattgg
    19141 gatgctgatc aactcatcgc aatgttcacg ggtcaggcga cgcatacctt caccttccgc
    19201 gcccatcacc agcgccaggc gtccggtcat tttgctctga tagagtgtat gatccgcctc
    19261 gcctgccgta ccgacgatcc agatattctc ttcctgcaac atacgcatgg tgcgcgcaag
    19321 gttagtcacc cgaatcagtg gaacgctttc tgccgcgccg caggctactt ttttcgccgt
    19381 agcgttgagc tgtgcggagc gatctttcgg cacaatcacc gcgtgaacgc cagcagcgtc
    19441 cgcgctacgc aggcacgcgc cgaggttgtg cggatcggtt acaccgtcga ggatcagcag
    19501 gaacggttga tcgagcgaag cgatcagatc cggcagatcg ttttcctgat actgacgtcc
    19561 tggcttcacg cgggcgataa tgccctgatg cacggcaccg tcgcttttct cgtcgagata
    19621 ttggcggttt gccaactgga taaccacgcc ctgagactca agggcgtgaa tcagcggtaa
    19681 cagacgttta tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt caggggcgcg
    19741 ctccagcagg gcctgcactg cgtggatgcc gtaaatcatt tcgctcatta atgtactcgt
    19801 tgtttacgtt ttgcctgatg cactacgttt atccggccta catgatctct gcaatatatt
    19861 gaatttgcgt gattatgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag
    19921 cgcattttgt cagcaatcta accctcttct tttaaagagg gtattgatca ctctgccact
    19981 tttttcttcg ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt tttcgccgat
    20041 ggctttttcg cttttctcgc gtctttcttc gccgctttcg gcttcgtttt tttctcaccg
    20101 cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct
    20161 tttttgcctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt
    20221 gcgcgttcac tggagatcag actaaagtcg attttgcgct cgtccatatt aaccgcttcg
    20281 actcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc ccccatcagg
    20341 cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccaga
    20401 ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt
    20461 acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcg
    20521 acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc
    20581 aacatctctt ccatcgaata atggtagccg ccggtttcag tggtgttgcc ctgatgcccc
    20641 tgctctttcg ccagcagata tttaatggcg cggtgcagcg tcaggtcagg ataacgacga
    20701 atcggcgaag taaagtgcgc ataggactgc aatgccagac cgaagtgacc acggttttct
    20761 ggatcgtaaa tcgcctgttt catcgagcgt agcagcatgg tttgcagcat ttctgcatca
    20821 ggacggtcgg caaccgactc cagcagttcc gcgtagtcac gcggttccgg cttgttacca
    20881 cccggcagct ccagccccag ctccgccagc actgaacgga aagaggtaat cgcttcggtg
    20941 ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc
    21001 gccgagatat tcgccagaat catgcactct tcaattaatt tgtgcgcgtc gttacgctgg
    21061 gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc
    21121 tcaaatgaga tcccaccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc
    21181 aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc
    21241 agaatatgcc agactttagt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag
    21301 aatttgtagc ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg
    21361 cggtctacct gcgggttgag cgaacacagg ccgttagaga gcacttccgg cagcatcggg
    21421 ataacctgcg aagggaagta caccgacgtg ccacggttac gcgcttctct gtccagcggc
    21481 gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccgccg
    21541 ccgcgttttt tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc gccatcaatg
    21601 gtgaccagcg gtaaatcgcg cagatcaaca cggcccgctt ttgcttcttc cggcacttct
    21661 tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg
    21721 gtacgcagag cgatatcaac cgccatgccg gtgcccatat tgtcgcccag cacttcgacg
    21781 attttaccca ccgctttggt gcggcgagtc ggacgctgag tcagttcgac tacgaccaca
    21841 aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg
    21901 ctatcgtcag gaaccacaaa gccgacgccc gcttcagtaa agtagcgacc aacaatctgg
    21961 ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagcg
    22021 cccagcggct gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag
    22081 agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca
    22141 ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg
    22201 aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc
    22261 tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt
    22321 ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct
    22381 tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag
    22441 attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg
    22501 caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt
    22561 tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg
    22621 gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccgattta cccaggcgaa
    22681 tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta
    22741 ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca
    22801 tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac
    22861 tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga
    22921 taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg
    22981 atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt
    23041 agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac ggatcgcgca
    23101 gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccagtca
    23161 gctcttcaat acgcttgatg tagttcagcg ccgcctgcgg cagaccgcta cgatctttca
    23221 cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc
    23281 agtcgtcagc tgccagcgga gtggtagtca cttcgcgacc atccggcata cggtaagcca
    23341 cgcagagttt aacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag
    23401 acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac
    23461 gacgacgccc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt
    23521 catcaaacag ttcggtcggg aacggacctg cacctacacg agttgagtaa gctttgagga
    23581 tacccagaac ataatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag
    23641 cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac
    23701 cctgcgcacc ttcgaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag
    23761 aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt
    23821 tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca
    23881 tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac
    23941 cgcgacgtgc tactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg
    24001 ctttcgcgcc acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa tcaaggatca
    24061 gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt
    24121 ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg
    24181 ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga
    24241 ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt
    24301 cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga
    24361 cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct
    24421 ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt
    24481 cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg
    24541 ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat
    24601 gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc
    24661 cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg
    24721 atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc
    24781 gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca
    24841 gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta
    24901 ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg catgatgcgg
    24961 ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt
    25021 ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg
    25081 cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttgatacgc
    25141 acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg
    25201 cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgctgccggg
    25261 gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg
    25321 gtcagacgac cacgggaatc ggtgacgatg tctttcacgt ccaggcgacc aatttcagaa
    25381 cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca
    25441 cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg
    25501 atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata
    25561 cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca
    25621 taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg
    25681 cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata
    25741 atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg
    25801 cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc
    25861 cactggttgt ggaggaagag gctggcggca aacgcagcag attgctggca ccgttatcgc
    25921 tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt
    25981 tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct
    26041 cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag
    26101 caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct
    26161 cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt
    26221 ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg
    26281 cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcaga gtgatgccca
    26341 tgtcatacgg acgaatcgtc tcttccagtt cgcgctgggt atcgctacga atcacggtac
    26401 gaccttccgt cagaatgcgg tccatggtgt atttaccgat aaccccgcgc agggcgctgt
    26461 cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat
    26521 tggtaacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca
    26581 tcacaccgga agcggccagt tcacgcacgg cttccacgtt taccggtttg acttcgtcga
    26641 taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg
    26701 ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga
    26761 caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg
    26821 atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc
    26881 tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc
    26941 catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt
    27001 tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg
    27061 ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg
    27121 taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga
    27181 acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga
    27241 taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc
    27301 tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt
    27361 gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa
    27421 tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc
    27481 tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca
    27541 atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgccgcc
    27601 accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg
    27661 acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc
    27721 gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat
    27781 cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc
    27841 tgcttttcaa ctctttccag gcgtgactgt atctgcacga tgcgattacg caacaaacga
    27901 cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt
    27961 tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca
    28021 acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg
    28081 gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc
    28141 agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca
    28201 actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc
    28261 acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc
    28321 atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa
    28381 cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc
    28441 cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
    28501 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt
    28561 actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
    28621 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
    28681 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
    28741 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
    28801 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
    28861 ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
    28921 cactgtcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
    28981 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa
    29041 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
    29101 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
    29161 aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
    29221 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
    29281 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
    29341 ctgccgcaac cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
    29401 ccgcctgttg ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc
    29461 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
    29521 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
    29581 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
    29641 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
    29701 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
    29761 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
    29821 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
    29881 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    29941 tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    30001 acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    30061 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    30121 cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc ggcagaaact
    30181 tttagccgca acggaatcag cagcggctgg gcgcaaacgg gcgcttcacc cggcgtcaat
    30241 tgtgcctgac gtagccaacg ttctgccact ggcaaggata aaagtgaaat gttgccatcg
    30301 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    30361 ttcgccgcaa gtgcaggttc ctgcggttcc ggcgctttta atttttgcat cggcgcgggc
    30421 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc
    30481 ggccaggggg cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctacc
    30541 ggctcacgag ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccggg
    30601 atggcacgcg gtgcgggttg tggttcatcg tccagcggta gcggcgtttc cagttgctgc
    30661 tgtagcacgc tcagcacgcc ctgatagata aagtcatgca ccagacgcga ctgatggaaa
    30721 cgcacttcgt gtttggcggg gtgtacgttg acgtccacct gatgcgggtc gatctccaga
    30781 tacaacacaa acgccggttg ttgatcggcc cccagtttgt cttcgcaggc ctggcggatc
    30841 gcatgattga tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc
    30901 gccagtgcgg gcgtagtgtg atttggatcg gccacccagc cgcgcagggt gagatcgccg
    30961 tgttgccatt caatcgccag cgcttgttcg agaaaagcgg tgccgcaaat cgcgcctaag
    31021 cgccgttctt tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta
    31081 tgcgacaggt tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatctcatca
    31141 atgtggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    31201 aacagatcca gcacctccag cgtcgtcccc acaggatgcg ccgccggttt taccgtcacg
    31261 ttcatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    31321 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    31381 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc
    31441 gccagcgcca gctcgtcttt tttgataccg cagccgttat cacgaatgcg gataagtttc
    31501 gccccaccgc gttcgatatc aatatcgata cgtgtcgcac ctgcatcgag gctgttttcc
    31561 accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    31621 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga
    31681 tctggcgtcg tcaccgtact ggccgtttgt gccgttgccc cctgcggcgc agattgcatc
    31741 ggatgcgcaa ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg
    31801 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    31861 agtactgatg ggatatccgg cgaacgcaga acgccgagac tcgcgtgttc tggtcggcgt
    31921 ttatgtattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    31981 acccgttggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac
    32041 tgactgttcg ccagcacatc acccgcccca cccagcagct ccgactgttt ctcgtgctgc
    32101 tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg
    32161 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt
    32221 tgcttacgtg ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt
    32281 aaaacgccct taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    32341 atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg
    32401 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
    32461 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    32521 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc
    32581 gcaaccacgg gcggcggagg aggaggtggc ggcgcatcgg cgttaatcgt aaagacgaca
    32641 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg
    32701 accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga
    32761 ttattgccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    32821 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    32881 gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
    32941 accggcgtgc acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc
    33001 ccgccacctt tcaaagatta accggctaaa cgcgccagca acaattcacc cgcagaggaa
    33061 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    33121 gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg
    33181 gcaaaataat cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    33241 tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg
    33301 cttttgacat taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtttta
    33361 cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc
    33421 cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg
    33481 gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    33541 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac cgtgcgcgac
    33601 acagcccgca caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    33661 accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat
    33721 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    33781 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    33841 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
    33901 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    33961 aaaattctca actttttgca gtgctttttt cccccactct tgctggccca gaccgggacc
    34021 aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatcg tcagttcatg
    34081 caccatcaat tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac
    34141 tcggactaaa ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc
    34201 cgtgccgtga tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg
    34261 gcgacgcggt tttagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc
    34321 ctgacctgcc agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc
    34381 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc
    34441 gttgatcact gcgcctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac
    34501 cgccgcaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    34561 gggcgcttgc tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
    34621 ccagacaata ttcgaagcat ggatctcgcc tcccgcgttt aaccatgctt cgcgtgccag
    34681 cgcggcctct tccggcaacg gtttgtcgct ctcctgggcc agcagcgtga cctcaatgcc
    34741 gaccgctgtg gccagccgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
    34801 cagcaccagc caatggcggg cgtcaggata cgccgaacga cacacctgga atgcggcttc
    34861 gccagcgcga agcatcagct catagagtgt aagccccagt gcatctgccg cctcacgttc
    34921 tccgcggcgg atatcgtcgg cgtgccagac ggtgtgtggt atacttacgg ggtttttctt
    34981 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    35041 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    35101 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    35161 ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt
    35221 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    35281 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    35341 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    35401 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    35461 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttctttct tctttttagg
    35521 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggtaa
    35581 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    35641 tgctcgccgc tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt
    35701 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg
    35761 gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc
    35821 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
    35881 atcggcgatt cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg
    35941 caatgcccct tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc
    36001 acttctcgat gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg
    36061 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt
    36121 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    36181 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta
    36241 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
    36301 cagcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac gaaaaccagc
    36361 aataacgcat cgcatcgctg tggatatttt attgagagaa gaatttggag cgggaaacga
    36421 gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga gctattcccg
    36481 cttgggtggt ctgtgctttg cagcactttt caaattttgg agcgggaaac gagactcgaa
    36541 ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc cgcgtactac
    36601 ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta cgggaggcgc attatacgag
    36661 aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg cgtttttatc tcaagtgctg
    36721 aaattatcag caagacgttc aatttagcgt caaaatcacg ccgccgcaag tggattccgg
    36781 cgcttacgtg accaggaaaa attttacagc ttgataaaat gctcgcggta gtaagccagc
    36841 tccgccaccg attcacggat atcatccatc gcctgatgcg tcccctgctt ggtaaaacca
    36901 tccagaattt ccggcttcca gcggcgcgcc agctctttca gggtgctgac atcgagataa
    36961 cggtagtgga agtaggcttc cagctccggc atgtatttaa acaggaaacg acggtcctga
    37021 ccgatgctgt taccgcaaat cggcgatttt cccgcaggca cccactgttt taaaaattcg
    37081 agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg ctttcacgcg ctctaccagc
    37141 ccgctggcgg tatgggtgcg cacgttccag tcatccatca gcgccagctg ttcatcagac
    37201 tggtgtactg caatggtcgg cccttctgcc agaatattca ggttggcatc ggtcaccagc
    37261 gtggcaatct caataatgcg atcgcgctcg ggatccagac cggtcatctc aagatcgatc
    37321 caaatcaggt tgttttcatt ggcactcatg ctattttcca cccattcaca gtgactatat
    37381 tgatccacaa ttgcgtgtat catagatgtt ttgcccatca ggggcgacca ggagtctgta
    37441 cgattgagta aaaataaact ctccaaaggc cagcagcgcc gcgtgaacgc caatcaccag
    37501 cgtcgtctta aaacgtctaa ggagaagccc gactacgacg acaatctgtt tggcgagcct
    37561 gatgaaggta tcgtcatcag ccgctttggt atgcacgctg atgtggaatc cgccgatggc
    37621 gacgttcacc gctgcaatat tcgccgtacc atccgttcgc tggtaaccgg cgaccgcgta
    37681 gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg tcaaagggat cgtggaagcg
    37741 gtgcatgagc gtacctcggt gttgacgcgt ccggatttct acgacggcgt aaaacctatt
    37801 gccgccaaca tcgaccagat tgtcattgtc tccgccattt tgccggagct gtcgctcaat
    37861 attatcgacc gttacctggt ggcctgcgaa accttgcaga ttgagccgat tattgtgctc
    37921 aacaagatag acctgctgga cgacgaaggc atggcgttcg tcaacgagca gatggatatc
    37981 taccgcaata tcggttatcg cgtattgatg gtttccagcc atactcagga tgggctaaaa
    38041 ccgctggaag aggcgttgac cgggcgcatc agcatttttg ccgggcagtc tggcgtcggc
    38101 aaatccagcc tgctgaatgc gttgctgggg ctgcaaaaag agatcctgac caacgatgtc
    38161 tctgacaact cggggctcgg tcagcacacc acgaccgccg ctcggctgta tcacttcccg
    38221 cacggcggtg atgtgattga ttcccctggt gtgcgtgagt tcggcctctg gcacctggag
    38281 ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact acttaggtct gtgtaaatat
    38341 cgcgattgca aacacgatac cgatccgggc tgcgctatcc gggaagcggt tgaggaaggg
    38401 aaaatcgcgg aaacccgttt cgaaaactat caccgtattc tggaaagcat ggcgcaggta
    38461 aaaacgcgta aaaacttttc tgatacggat gactgacaat taagctaacc gtcgttagaa
    38521 tcgtcccctt tttttcagga tccggcatgt aggccggatc aggaacgaca aaacaatggc
    38581 ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac agtacattct gccgaaacta
    38641 tggcttactc gcctggcggg ttggggcgca agcaagcggg caggatggct gacaaaactg
    38701 gttatcgatc tgttcgttaa atactacaag gtcgacatga aagaggcgca aaagccggac
    38761 accgccagct accgcacctt taacgaattc tttgtccgtc cgctgcgtga cgaagtacgc
    38821 ccaatcgata ccgatccgaa tgtcctggtc atgcctgccg atggcgttat cagccagttg
    38881 ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc acaactacag cctcgaagcc
    38941 ttgctggcag gcaactatct gatggcggac ctgttccgca acggtacgtt tgtgaccact
    39001 tacctctccc cgcgtgacta ccaccgcgta cacatgccgt gcaacggtat tctgcgtgag
    39061 atgatctacg tgccgggcga tctcttctcc gttaaccatc tcaccgctca gaacgtgccg
    39121 aatctgtttg cccgtaacga acgcgtgatt tgccttttcg ataccgaatt tggcccaatg
    39181 gcgcagattc tggtcggagc gacgattgtt ggcagcattg agacggtctg ggcgggcacc
    39241 attacgccgc cgcgcgaagg tatcatcaag cgctggacct ggcctgccgg ggaaaacgac
    39301 ggttctgtgg cactgctgaa aggccaggaa atgggtcgct ttaaactcgg ttccaccgtt
    39361 atcaacctgt ttgcaccggg taaagtgaat ctggttgagc aactggaaag cctgtctgtc
    39421 acgaaaattg gccagccgct ggcagtatct accgaaacct ttgttacgcc agacgctgaa
    39481 cctgccccgc ttcctgctga agagatcgaa gcagaacacg acgccagccc attggttgac
    39541 gacaaaaaag accaggtcta atccatcaaa ggaaacgctg acgtgcgcct gattatcact
    39601 tttctgatgg cctggtgcct cagttggggg gcgtacgccg cgacggcccc cgatagcaaa
    39661 caaatcactc aggaactgga gcaggcaaaa gcggcgaaac ccgcacagcc ggaagtcgta
    39721 gaggcgctcc agtctgcctt aaatgcgctt gaggaacgaa aaggttccct tgagcgcatc
    39781 aaacaatatc agcaagttat cgataattat ccgaaactct ccgctactct gcgcgcacaa
    39841 ttaaacaaca tgcgtgacga gccgcgcagc gtgtcgccgg gaatgtctac cgacgcgctg
    39901 aatcaggaaa ttctccaggt cagcagccag ttgctggata aaagccgtca ggcccagcaa
    39961 gagcaggagc gcgcccgcga gattgccgat tcgctgaatc aactgccgca acagcaaacc
    40021 gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg ggacgcttac tggcaatact
    40081 ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact ctgcacgtct taaggcgctc
    40141 gttgatgaac tggagctggc gcagctgtct gccaataacc gccaggaatt agcgcgctta
    40201 cgctcagagc tggcggaaaa agagagccag caactggatg cgtatttgca ggccttgcgt
    40261 aatcaattaa acagccaacg tcagcttgag gcggagcggg cgctggaaag taccgaatta
    40321 ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg cgcaattcaa aattaaccgc
    40381 gaactatcgg cggctttgaa tcaacaggcg cagcggatgg atctcgttgc ctcgcaacag
    40441 cgtcaggctg ccagccagac gttacaggtc cggcaggcgt tgaatacgct gcgtgaacag
    40501 tcgcaatggc tgggatcgtc caatctgctc ggcgaagcgc tgcgggcgca ggtggcacgg
    40561 ctgccagaaa tgccaaaacc acaacagctt gataccgaaa tggcgcagtt gcgtgtgcaa
    40621 cggttacgtt atgaggatct gcttaataaa cagccgctgc tacggcaaat ccatcaggcc
    40681 gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg aagcacaact gcgcactcag
    40741 cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc tactattgga actgaccaag
    40801 ctgaaagtct ccaacgggca actggaggat gcgctgaaag aggtgaacga agcaacgcac
    40861 cgctatctgt tctggacctc tgacgtgcgc ccgatgacca tcgcctggcc gctggaaatc
    40921 gcccaggatc tgcgtcgtct catttcgctg gacaccttca gtcagttggg caaagccagt
    40981 gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt ttggcgcgtt gattctggtc
    41041 ggttgcagta tttactcgcg ccgctatttc acccgttttc ttgaacgttc ggcggcgaaa
    41101 gttggcaaag tgactcagga tcacttctgg ctgacgttgc gcactctttt ctggtcgatt
    41161 ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg gttacggctt gcgcgaggcg
    41221 tggccttatc cgctggcggt cgcgattggc gatggtgtaa cggccaccgt gccgctgctg
    41281 tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg gcttgtttat cgctcatttt
    41341 ggctggccgc gcgaacgtgt ttcccgtggg atgcgctact acctgatgag catcgggctt
    41401 attgtgccgc tgattatggc gctgatgatg ttcgataacc tcgacgaccg tgaattctcc
    41461 ggttcgctgg gacggctttg ctttatcctc atttgcggtg cgctggcggt ggtcaccctc
    41521 agcctgaaaa aggccgggat cccactgtat ctcaacaaag agggcagcgg cgacaacatt
    41581 accaaccata tgctgtggaa catgatgatt ggcgcgccac tggttgccat tctggcgtcg
    41641 gcggtgggtt atctggcaac ggcacaggcg ctgttagcga ggcttgaaac ctcggttgcc
    41701 atctggttcc tgctactggt ggtttatcat gttatccgcc gctggatgct gatccaacgc
    41761 cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag agatgttagc gcaacgtgcg
    41821 cgtggcgaag aggaagcaca tcatcacagt agcccggaag gggcaattga agtcgatgaa
    41881 agcgaagtcg atctcgatgc catcagtgcg caatccttgc ggctggtgcg ctcaattttg
    41941 atgttgatcg ccctgctttc ggtcattgtg ctgtggtcag aaatccattc cgctttcggc
    42001 ttcctcgaaa atatttcgct gtgggatgtc acctccacgg tacagggcgt agaaagtctg
    42061 gagccaatta ccctcggtgc ggtgctgatt gccattctgg tgtttatcat caccacgcag
    42121 ctggtgcgca acctgcccgc gctgctggaa ctggcgattt tgcagcacct ggatttaacg
    42181 ccgggtacgg gttacgccat caccaccatc accaaatatc tgctgatgct gattggcggg
    42241 ctggtcggct tctcaatgat tggtattgag tggtcgaaat tgcagtggct ggttgccgcg
    42301 ctcggtgttg gtctcggttt tggtttacag gaaattttcg ccaactttat ctctggcctg
    42361 atcatcctgt tcgaaaaacc gattcgcatt ggcgatacgg taacgatccg cgatcttact
    42421 ggtagcgtga cgaaaattaa tacccgcgca accaccatca gcgactggga ccgtaaagag
    42481 ataatcgtgc cgaacaaggc gtttattacc gagcagttta tcaactggtc gctctctgac
    42541 tcggtcacgc gcgtggtgtt gacgattccg gcccctgccg atgccaacag tgaagaagtg
    42601 acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg tgatcgacaa cccggcaccg
    42661 gaagtcttcc tggtggatct gcaacaaggg attcagattt tcgagctgcg tatttacgcc
    42721 gctgagatgg gtcaccgtat gccgctacgc catgagatcc accagctgat tctggcaggc
    42781 ttccatgccc acggtatcga tatgccattc ccgcccttcc agatgcgtct ggaaagcctg
    42841 aatggtaaac aaacggggag aacattaact tctgcgggca aaggtcgtca ggcgggaagt
    42901 ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc atttagcagc ttaacatcat
    42961 cttcagttat caccggccct ggagagtatc atccatacgc attacttcct gacaactcat
    43021 cgccggagag acgatgagtt gcaggatttc atatgatcag tcgagagcta actcaggttt
    43081 attcgccatg cgactgcaat agttctggta aatcgccatc gctaacagcg agaagaagac
    43141 agggccgcca atcatccaca atgtgctgtc ccagtctcca gcttcaacca caggttgaat
    43201 gatggtgaag acgttcgcaa atgtcaccac cagtacgaca accaccgtcg cgatcattgc
    43261 cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga tcctgacgtg ctttaaagaa
    43321 tgggaaagcc agcgcgagga acaggtaagg aagcgtcata gacacgttcg ccatcagcgt
    43381 cagcttgtta aagaacgccg atgcggtacc gccaccaaac gaaaccagca gaatgaagat
    43441 agtaaccaac ccgcactgca tccacatggc gatagacggc atccccatcg cattcaggcg
    43501 cgtcatcggt tccggccaca atgctttcgg cgtcccctgg atgatggctt tcaacggtga
    43561 atagcaaagc gtaaagaacg caccggtata ggcgaggaac atcgaaagtc cagtaatacg
    43621 cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac aaatgcagtg cattacccag
    43681 cgtcatccca aggctcttca tcagcacata ggtaatattg ccgaggttaa cggaaccatt
    43741 acttaatacc tgctgccagt ttgtgctgac gccccataaa aatattgcca gcgaataacc
    43801 gattgaaata acaatagcgg caaaaacaat acctttggca aagttctttt ctggattttc
    43861 cgttttatcg accagaccac caaccgcttc aattccgcca taggcaaaaa tggcaaatac
    43921 cacaaacgat agcattgcca gaccggactg ataacccggg ttcggtgatg caaggaaatt
    43981 aatatcctgc gcgaaatgcc caccatttaa taacaaaata gtaatgctta ctaacagcaa
    44041 tactaaattc agacacatta ctgcaatacc gcccaccgca gtaatgcggg caattttatt
    44101 aatcccctta gaagcaacga cggtgaccag aatcatccat gccactgcca gcagaccaac
    44161 cacctgcgta ggctccagtc cggcaatacg ccagtgctgg gtcatgtcgc taccatagag
    44221 gaatgttgag aacggtaccc aaactttcgc ggaggtactc accatccaga tgatataaga
    44281 ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt ccgacactat tattcatcca
    44341 ggaatagata ccaccttctt ctttgcgata agcagctccc atttcagcca tcattaaggc
    44401 gaatggaata aagaataaca atgcagaaaa tatataaaag ggaatcgcac tataacccat
    44461 taagtaataa gccgatgggc tattggcaaa accaaatacg gaagtaaaga tcatcaatat
    44521 gagtcctatc agactcattt tttttatcgt gtgaggcatg aaaccatcct tcatttcagc
    44581 gcgtgtataa cacgtgccca ctgaactgca ctgcgcaaca ttgagcaggc agcgtaaaaa
    44641 ccaatacaga attgatacct ggatgatatc aaatatccag tcttaaattg tggcctttag
    44701 cggaaaaatg aaaacatatg ctacagaatg gcgcttatta cgccattctt cgctgttaat
    44761 tcagtaattt ttcagaatta tgcccggtca acgctaaagg cgatgacttc agccagtgtc
    44821 tccgcgccca gcgccaacat caccagacga tcaacaccta atgccacgcc ggaacagtca
    44881 ggcataccga ctttcaaggc gtcaatcaga ttctggtcaa tggggtgctg cggcaaaccg
    44941 cgcgccgcgc gcttacggtt atcttgttca aagcgttgtt gctgctcacg ggcatccgtc
    45001 aattcatgga aaccattcgc cagctcaata cctttataat aaacctcaaa gcgttcagcg
    45061 acccgatgat cttcggtact gatttgcgcc agtgatgcct ggctggctgg aaagtggtac
    45121 acaaaggtcg gtttttcttt accaatattt ggctctacgc caaaggtaaa cagcaattgt
    45181 agcaacgtgt cgcggtcttc ttcggtatca gcaacattgc tcaaatccag tttcgctgcc
    45241 acttcccgca gttgcgtttt gtcggcagag agtgggtcaa tttccagata acgcaagaaa
    45301 gcttgttgat aagaaaggct ttctgctgcc gggcagtcca gcacctgttg taagagatcg
    45361 tccacctcgt tcatcaaccg gtacatatca tagtgcggtc gataccactc cagcatagtg
    45421 aactcagggt tgtgataacg ccccatctct tcattacgga agctgcggca cagctggaat
    45481 accggcccac aaccggcaac cagcaggcgt ttcatatggt attccgggct ggtcattaac
    45541 cagagattca tcccctgcga atgcccgggg ccaacgaaac gtgtctcaaa cgggaccaaa
    45601 tgaatatcgg ttaccgtcgc ctggctcata caaggcgtct ccacctccag cactccacga
    45661 tcggcaaaga aacgacggat ctccgccata atcgccgcgc gttttaataa gttaggaatg
    45721 gatgcgctcg gctgccagga tgccgtttcg ctcatagtta aatctccagt ttttgacaag
    45781 ggcacgaagt ctactcgcaa cgcgacggcg agacaaattt tacgcaggaa tcaaacagcg
    45841 gtgggcagtg actaaaaaaa gcacgatctg atggtttagt aattaaatta atcatcttca
    45901 gtgataattt agccctcttg cgcactaaaa aaatcgatct cgtcaaattt cagacttatc
    45961 catcagacta tactgttgta cctataaagg agcagtggaa tagcgttcgc agaccgtaac
    46021 tttcaggtac ttaccctgaa gtacgtggct gtgggataaa aacaatctgg aggaatgtcg
    46081 tgcaaacctt tcaagccgat cttgccattg taggcgccgg tggcgcggga ttacgtgctg
    46141 caattgctgc cgcgcaggca aatccgaatg caaaaatcgc actaatctca aaagtatacc
    46201 cgatgcgtag ccataccgtt gctgcagaag ggggctccgc cgctgtcgcg caggatcatg
    46261 acagcttcga atatcacttt cacgatacag tagcgggtgg cgactggttg tgtgagcagg
    46321 atgtcgtgga ttatttcgtc caccactgcc caaccgaaat gacccaactg gaactgtggg
    46381 gatgcccatg gagccgtcgc ccggatggta gcgtcaacgt acgtcgcttc ggcggcatga
    46441 aaatcgagcg cacctggttc gccgccgata agaccggctt ccatatgctg cacacgctgt
    46501 tccagacctc tctgcaattc ccgcagatcc agcgttttga cgaacatttc gtgctggata
    46561 ttctggttga tgatggtcat gttcgcggcc tggtagcaat gaacatgatg gaaggcacgc
    46621 tggtgcagat ccgtgctaac gcggtcgtta tggctactgg cggtgcgggt cgcgtttatc
    46681 gttacaacac caacggcggc atcgttaccg gtgacggtat gggtatggcg ctaagccacg
    46741 gcgttccgct gcgtgacatg gaattcgttc agtatcaccc aaccggtctg ccaggttccg
    46801 gtatcctgat gaccgaaggc tgccgcggtg aaggcggtat tctggtcaac aaaaatggct
    46861 accgttatct gcaagattac ggcatgggcc cggaaactcc gctgggcgag ccgaaaaaca
    46921 aatatatgga actgggtcca cgcgacaaag tttctcaggc cttctggcac gaatggcgta
    46981 aaggcaacac catctccacg ccgcgtggcg atgtggttta tctcgacctg cgtcacctcg
    47041 gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga actggcgaaa gcgtacgttg
    47101 gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac cgcacactac accatgggcg
    47161 gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc gtgggtgaat
    47221 gttcctctgt tggtctgcac ggtgcaaacc gtctgggctc caactccctg gcggaactgg
    47281 tggtcttcgg ccgtctggcc ggtgaacaag cgacagagcg tgcagcaact gccggtaatg
    47341 gcaacgaagc ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg aaagatctgg
    47401 ttaaccagga tggcggcgaa aactgggcga agatccgcga cgaaatgggc ctggcaatgg
    47461 aagaaggttg cggtatctac cgtacgccgg aactgatgca gaaaaccatc gacaagctgg
    47521 cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga cacctccagc gtgttcaaca
    47581 ccgacctgct ctacaccatt gaactgggcc acggtctgaa cgttgctgaa tgtatggcgc
    47641 actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac gaaggttgca
    47701 ccgagcgtga cgacgtcaac ttcctcaaac acaccctcgc cttccgcgat gctgatggca
    47761 cgactcgcct ggagtacagc gacgtgaaga ttactacgct gccgccagct aaacgcgttt
    47821 acggtggcga agcggatgca gccgataagg cggaagcagc caataagaag gagaaggcga
    47881 atggctgaga tgaaaaacct gaaaattgag gtggtgcgct ataacccgga agtcgatacc
    47941 gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt actggatgcg
    48001 ctgggctaca tcaaagacaa cctggcaccg gacctgagtt accgctggtc ctgccgtatg
    48061 gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc caaaactggc atgtaaaacc
    48121 ttcctgcgtg attacaccga cggtatgaag gttgaagcgt tagctaactt cccgattgaa
    48181 cgcgatctgg tggtcgatat gacccacttc atcgaaagtc tggaagcgat caaaccgtac
    48241 atcatcggca actcccgcac cgcggatcag ggtactaaca tccagacccc ggcgcagatg
    48301 gcaaagtatc accagttctc cggttgcatc aactgtggtc tgtgctacgc cgcgtgcccg
    48361 cagtttggcc tgaacccaga gttcatcggt ccggctgcca ttacgctggc gcatcgttat
    48421 aacgaagata gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa cagccagaac
    48481 ggcgtatgga gctgtacttt cgtgggctat tgctccgaag tctgcccgaa acacgtcgat
    48541 ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct tatcgcgacc
    48601 ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg tacggccaat
    48661 gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc gcgaaggcac
    48721 ggcggttccg gctgtgtggt tcagcattga actgattttc gggctgtttg ccctgaaaaa
    48781 tggcccggaa gcctgggcgg gattcgtcga ctttttacaa aacccggtta tcgtgatcat
    48841 taacctgatc actctggcgg cagctctgct gcacaccaaa acctggtttg aactggcacc
    48901 gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa ttatcaaaag
    48961 tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc tgtactggta
    49021 aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta ttctggggcc
    49081 tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc ctgctggtgg
    49141 gtattctgct gccactgggg ctgtttccgg gtgatgcgct gagctacgag cgcgttctgg
    49201 cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt
    49261 ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaattcac gtgcctgcgg
    49321 gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg attggtatcg
    49381 ttacaatcta acgtatcgcc aatgtaaatc cggcccgcct atggcgggcc gttttgtatg
    49441 gaaaccagac cttatgttca aaacgacgct ctgcgcctta ttaattaccg cctcttgctc
    49501 cacatttgct gcccctcaac aaatcaacga tattgtgcat cgcacaatta ccccgcttat
    49561 agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta aaccttatta
    49621 ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc aaacgttgtt
    49681 tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgacg ctattgctcg
    49741 aggggaaatc aagttaagcg atcccgcaac aaaatactgg cctgaactta ccgctaaaca
    49801 gtggaatggg atcacactat tacatcttgc gacctacacc gctggcggcc tgccattgca
    49861 ggtgccggat gaagtgaaat cctcaagcga cttgctgcgc ttctatcaaa actggcagcc
    49921 tgcatgggct ccaggaacac aacgtctgta tgccaactcc agtatcggtt tgttcggcgc
    49981 actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaactc gtgtcttcca
    50041 gccactcaaa ctcaaccata cgtggattaa tgtacctccc ccagaagaaa agaattacgc
    50101 ctggggatat cgcgaaggta aggcagtgca tgtttcgcca ggggcgttag atgctgaagc
    50161 ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg gtacgaagca atatgaatcc
    50221 ccgtgatatc aacgacaaaa cacttcagca agggatacaa ctggcacaat ctcgctactg
    50281 gcaaaccggc gatatgtatc agggcctggg ctgggaaatg ctggactggc cggtaaatcc
    50341 tgacagcatc attaacggca gtggcaataa aattgcactg gcagcacacc ctgtaaaagc
    50401 gattacgccc ccaactcctg cagtacgcgc atcatgggta cataaaacag gggcaaccgg
    50461 cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatag tgatgctggc
    50521 taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tggcagattc tcaacgctct
    50581 acagtaaaat ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc cttgctgtca
    50641 tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct ctcgttgccg
    50701 cagcgacagc tgcatttctg gtcgttgcct gcagttctcc tacgccgccg cgtggcgtaa
    50761 cagtagtgaa taatttcgac gcaaaacgtt atctcggtac ctggtatgag attgcccgtt
    50821 ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc aacatacagt ctgcgtgatg
    50881 acggcggcct gaatgtcatc aataaaggct ataaccctga cagagaaatg tggcagcaga
    50941 gtgaagggaa agcgtacttt accggcgcac caacacgcgc tgcgctgaaa gtgtcattct
    51001 ttggtccttt ctatggcggt tataacgtca ttgcacttga tcgggaatac cgccatgcgc
    51061 tggtttgcgg cccggaccgc gactacctgt ggatactctc ccgcacgcca accatttctg
    51121 acgaagtgaa acaggagatg ctggcagtcg cgacccggga agggtttgat gtcagtaagt
    51181 ttatttgggt acagcagccc ggtagttagt gagtgctgag tttcagacca ataatcccca
    51241 atacgattaa cgccagactc gccaggcgca tcgggttagc ggactcaccg agcagcacaa
    51301 tgccggtgat ggccgcgccg actgcgccaa tacccgtcca cacggcataa gccgtcccta
    51361 ccggtaacga tttcatcgcc caggcaagta gcgccatact gactatcatc gccgtcacgg
    51421 taataacact cggcgtcaaa cggctaaagc cgtgggtata tttcaggcca acggcccata
    51481 ccacttccag cagaccagca ataactaaga taatccagga catatcaggc tccggagaat
    51541 tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc ctgcaaagct gatggttgaa
    51601 cggatatttt gccaggtggc aggggaattt caactattac ctttgtgact atcgcaaaaa
    51661 aacaaacggc acgacataac tgtcgtaccg ttattgctta ttgctgcgct ttcgttgctg
    51721 caccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc
    51781 aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca
    51841 ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag
    51901 acaacatcgc caaaaatgat ggaaatctga tgatttttcg gaagagaaga aaaattagct
    51961 ggcagcgcgg gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt
    52021 gttacagccc gtgagcagcg tgctggcaag caaaacaaga acgataaggc gtttcatcat
    52081 ctctgacctg aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac
    52141 gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa
    52201 cagcggaact ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc
    52261 ggtatcacct ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg
    52321 agtaacggag atcggctgac cattccacag agttacgata cactctgcct gatccagcag
    52381 ccatttagcg ttgtcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt
    52441 gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac
    52501 atcagcgcct tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag
    52561 cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact
    52621 cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt
    52681 gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa
    52741 tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg
    52801 atgttgtgac cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac
    52861 tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg
    52921 atcgcatgga gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag
    52981 atgagtttgt cgtcgcgtcc ggattctcca ccgacccgct ggaagaacag cacagcgtag
    53041 tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg
    53101 cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca
    53161 agcgtaactg gcagactgct cttgagtatg ttgctgcgca tccggaactg gacgagatga
    53221 ttttctccgg cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac
    53281 aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga
    53341 tcccggcacg tatcaccgag gcgctggttg aacgctttgc ccgttctacg ctgcaaatct
    53401 tgctggtgaa tcacatcaat catgccaatg aggtagatga aacgttccgt caggcgatgg
    53461 ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga
    53521 acgataacgc acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtaatgc
    53581 cgtattacct gcatgtgctc gataaagtac agggcgcggc gcattttatg gtgagtgatg
    53641 acgaagcacg gcagattatg cgtgagttgc tgacactggt gtcgggatat ctggtgccga
    53701 aactggcgcg agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc
    53761 agcagtaact gattaaacag ggaagtcaaa cattaaaatt gcctgatgcg ctacgcttat
    53821 caggcctaca tcgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg
    53881 cgtttacgcc gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg
    53941 ggaaatttaa tctttgcccg ttgcataacc tttaattatt catatacatt gctgttgtta
    54001 ctctttatat aactaacacc aataattaac ctttcttttc tgtttatata gagtgagtac
    54061 ggctattta
//
LOCUS       DAPHJE010000011        53760 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPHJE010000011 DAPHJE010000000
VERSION     DAPHJE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.011"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            256..1350
                     /gene="gcvT"
                     /locus_tag="RH222_000728"
     CDS             256..1350
                     /gene="gcvT"
                     /locus_tag="RH222_000728"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV3795789.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMGETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1374..1763
                     /gene="gcvH"
                     /locus_tag="RH222_000729"
     CDS             1374..1763
                     /gene="gcvH"
                     /locus_tag="RH222_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV3795790.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1881..4754
                     /gene="gcvP"
                     /locus_tag="RH222_000730"
     CDS             1881..4754
                     /gene="gcvP"
                     /locus_tag="RH222_000730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV3795791.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5020..5763
                     /locus_tag="RH222_000731"
     CDS             5020..5763
                     /locus_tag="RH222_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3795792.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5820..7253)
                     /gene="bglA"
                     /locus_tag="RH222_000732"
     CDS             complement(5820..7253)
                     /gene="bglA"
                     /locus_tag="RH222_000732"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV3795793.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            7298..7609
                     /locus_tag="RH222_000733"
     CDS             7298..7609
                     /locus_tag="RH222_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3795794.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7773..8432
                     /locus_tag="RH222_000734"
     CDS             7773..8432
                     /locus_tag="RH222_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV3795795.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8509..9489)
                     /gene="ygfZ"
                     /locus_tag="RH222_000735"
     CDS             complement(8509..9489)
                     /gene="ygfZ"
                     /locus_tag="RH222_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV3795796.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9732..9998
                     /gene="sdhE"
                     /locus_tag="RH222_000736"
     CDS             9732..9998
                     /gene="sdhE"
                     /locus_tag="RH222_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV3795797.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9979..10386
                     /locus_tag="RH222_000737"
     CDS             9979..10386
                     /locus_tag="RH222_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HDV3795798.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10426..10947)
                     /gene="fldB"
                     /locus_tag="RH222_000738"
     CDS             complement(10426..10947)
                     /gene="fldB"
                     /locus_tag="RH222_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV3795799.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11059..11955
                     /gene="xerD"
                     /locus_tag="RH222_000739"
     CDS             11059..11955
                     /gene="xerD"
                     /locus_tag="RH222_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV3795800.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11980..12690
                     /gene="dsbC"
                     /locus_tag="RH222_000740"
     CDS             11980..12690
                     /gene="dsbC"
                     /locus_tag="RH222_000740"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV3795801.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12696..14429
                     /gene="recJ"
                     /locus_tag="RH222_000741"
     CDS             12696..14429
                     /gene="recJ"
                     /locus_tag="RH222_000741"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV3795802.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14520..15618
                     /gene="prfB"
                     /locus_tag="RH222_000742"
     CDS             join(14520..14594,14596..15618)
                     /gene="prfB"
                     /locus_tag="RH222_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV3795803.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15628..17145
                     /gene="lysS"
                     /locus_tag="RH222_000743"
     CDS             15628..17145
                     /gene="lysS"
                     /locus_tag="RH222_000743"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV3795804.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17188..17736)
                     /gene="idi"
                     /locus_tag="RH222_000744"
     CDS             complement(17188..17736)
                     /gene="idi"
                     /locus_tag="RH222_000744"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV3795805.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17791..17862
                     /locus_tag="RH222_000745"
     CDS             17791..17862
                     /locus_tag="RH222_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795806.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKSI"
     gene            complement(17859..17984)
                     /locus_tag="RH222_000746"
     CDS             complement(17859..17984)
                     /locus_tag="RH222_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795807.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17986..19434)
                     /gene="uacT"
                     /locus_tag="RH222_000747"
     CDS             complement(17986..19434)
                     /gene="uacT"
                     /locus_tag="RH222_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV3795808.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19870..21789
                     /gene="ygfT"
                     /locus_tag="RH222_000748"
     CDS             19870..21789
                     /gene="ygfT"
                     /locus_tag="RH222_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV3795809.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSNL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21789..22277
                     /locus_tag="RH222_000749"
     CDS             21789..22277
                     /locus_tag="RH222_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV3795810.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22313..23680)
                     /gene="ghxQ"
                     /locus_tag="RH222_000750"
                     /gene_synonym="ygfQ"
     CDS             complement(22313..23680)
                     /gene="ghxQ"
                     /locus_tag="RH222_000750"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV3795811.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23716..25032)
                     /gene="guaD"
                     /locus_tag="RH222_000751"
     CDS             complement(23716..25032)
                     /gene="guaD"
                     /locus_tag="RH222_000751"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV3795812.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25050..26450)
                     /gene="xanQ"
                     /locus_tag="RH222_000752"
     CDS             complement(25050..26450)
                     /gene="xanQ"
                     /locus_tag="RH222_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV3795813.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26615..29485)
                     /locus_tag="RH222_000753"
     CDS             complement(26615..29485)
                     /locus_tag="RH222_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV3795814.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29482..30261)
                     /locus_tag="RH222_000754"
     CDS             complement(29482..30261)
                     /locus_tag="RH222_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV3795815.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLASPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(30312..31640)
                     /gene="ssnA"
                     /locus_tag="RH222_000755"
     CDS             complement(30312..31640)
                     /gene="ssnA"
                     /locus_tag="RH222_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV3795816.1"
                     /translation="MLILKNVTAVQLHPAKVQEAVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFAAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            complement(31643..34741)
                     /gene="ygfK"
                     /locus_tag="RH222_000756"
     CDS             complement(31643..34741)
                     /gene="ygfK"
                     /locus_tag="RH222_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV3795817.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(35063..35641)
                     /gene="mocA"
                     /locus_tag="RH222_000757"
     CDS             complement(35063..35641)
                     /gene="mocA"
                     /locus_tag="RH222_000757"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV3795818.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            35744..36514
                     /gene="yqeC"
                     /locus_tag="RH222_000758"
     CDS             35744..36514
                     /gene="yqeC"
                     /locus_tag="RH222_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV3795819.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36562..38187
                     /locus_tag="RH222_000759"
     CDS             36562..38187
                     /locus_tag="RH222_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV3795820.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38228..39160)
                     /gene="arcC"
                     /locus_tag="RH222_000760"
     CDS             complement(38228..39160)
                     /gene="arcC"
                     /locus_tag="RH222_000760"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV3795821.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39208..40593)
                     /gene="hyuA"
                     /locus_tag="RH222_000761"
     CDS             complement(39208..40593)
                     /gene="hyuA"
                     /locus_tag="RH222_000761"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV3795822.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40646..41857)
                     /locus_tag="RH222_000762"
     CDS             complement(40646..41857)
                     /locus_tag="RH222_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV3795823.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41915..43111)
                     /gene="dpaL"
                     /locus_tag="RH222_000763"
     CDS             complement(41915..43111)
                     /gene="dpaL"
                     /locus_tag="RH222_000763"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV3795824.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43169..44356)
                     /gene="ygeW"
                     /locus_tag="RH222_000764"
     CDS             complement(43169..44356)
                     /gene="ygeW"
                     /locus_tag="RH222_000764"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV3795825.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            44835..46613
                     /locus_tag="RH222_000765"
     CDS             44835..46613
                     /locus_tag="RH222_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV3795826.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(46653..47132)
                     /gene="xdhC"
                     /locus_tag="RH222_000766"
     CDS             complement(46653..47132)
                     /gene="xdhC"
                     /locus_tag="RH222_000766"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV3795827.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(47129..48007)
                     /gene="xdhB"
                     /locus_tag="RH222_000767"
     CDS             complement(47129..48007)
                     /gene="xdhB"
                     /locus_tag="RH222_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV3795828.1"
                     /translation="MFDFASYHRAATLADAINLLVDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(48018..50315)
                     /gene="xdhA"
                     /locus_tag="RH222_000768"
     CDS             complement(48018..50315)
                     /gene="xdhA"
                     /locus_tag="RH222_000768"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV3795829.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            50730..51485
                     /locus_tag="RH222_000769"
     CDS             50730..51485
                     /locus_tag="RH222_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV3795830.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            51564..51637
                     /locus_tag="RH222_000770"
     tRNA            51564..51637
                     /locus_tag="RH222_000770"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51596..51598,aa:Gly,seq:ccc)
     gene            51871..53481
                     /locus_tag="RH222_000771"
     CDS             51871..53481
                     /locus_tag="RH222_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795831.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHVKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPNP
                     IIPLNWSKASMTLMLKEMSSLINDEGNK"
     gene            53548..53727
                     /locus_tag="RH222_000772"
     CDS             53548..53727
                     /locus_tag="RH222_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDV3795832.1"
                     /translation="MINKLLLAYLIGLVVTSTFIFIFSEEKVTYRLFATIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        13288 a        13289 c        14020 g        13163 t
ORIGIN      
        1 ctttctcatc acatcatctt tatataaaaa cttgtgtatt ttttggtttt ttattctgtt
       61 gcgattttgg cgttttttaa ccataagcta atgtgatgat caattttacc ttatggttaa
      121 cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg accgggagtg
      181 gctccgatgc tgggtttcgt ggtgataatt tcactatgaa aaagttgtca gccccgctta
      241 ttcaatgagg acaagatggc acaacagact cctttgtacg aacaacatac gctttgcggc
      301 gctcgcatgg tggatttcca cggctggatg atgccgctgc attacggttc gcaaatcgac
      361 gaacatcatg cggtacgtac cgatgccgga atgtttgatg tgtcacatat gaccatcgtc
      421 gatcttcgcg gcagccgcac ccgggagttt ctgcgttatc tgctggcgaa cgatgtggcg
      481 aagctcacca aaagcggcaa agccctttac tcggggatgt tgaatgcctc tggcggtgtg
      541 atagatgatc tcatcgtcta ctactttact gaagatttct tccgcctcgt tgttaactcc
      601 gccacccgcg aaaaagacct ctcctggatt acccaacacg ctgaaccttt cggcatcgaa
      661 attaccgttc gtgatgacct ttccatgatt gccgtacaag ggccgaatgc gcaggcaaaa
      721 gctgccacac tgtttaatga tgctcagcgt caggcggtgg aagggatgaa accgttcttt
      781 ggcgtgcagg cgggcgatct gtttattgcc accactggtt ataccggtga agcgggctat
      841 gaaattgcgc tgcccaatga aaaagcggcg gatttctggc gcgcgctggt ggaagcgggc
      901 gttaagccat gcggcctggg cgcgcgtgac acgctgcgtc tggaagcggg catgaatctt
      961 tatggtcagg agatgggcga aactatttct cctttagccg ccaacatggg ctggactatc
     1021 gcctgggaac cggcagatcg tgacttcatc ggtcgtgaag ccctggaagt gcagcgtgag
     1081 catggcacag aaaaactggt tggtctggtg atgaccgaaa aaggcgtgct gcgtaatgaa
     1141 ctgccggtac gttttaccga tgcgcagggc aaccagcatg aaggcattat caccagcggg
     1201 actttctccc cgacgctggg ttacagcatt gcgctggcac gcgtgccgga aggtattggc
     1261 gaaacggcga ttgtgcaaat tcgcaaccgt gaaatgccgg ttaaagtgac gaaacctgtt
     1321 tttgtgcgta acggtaaagc cgtcgcgtga tttacttttt tggagattga ttgatgagca
     1381 acgtaccagc agaactgaaa tacagcaaag aacacgaatg gctgcgtaaa gaagccgacg
     1441 gcacttacac cgttggtata accgaacatg cccaggagct gttaggcgat atggtgtttg
     1501 ttgacctgcc ggaagtgggc gcaacggtta gcgcgggcga tgactgcgcg gttgccgaat
     1561 cagtaaaagc ggcgtcagac atttatgcgc cagtaagcgg tgaaatcgtg gcggtaaacg
     1621 acgcactgag cgattccccg gaactggtga acagcgaacc gtatgcaggt ggctggatct
     1681 ttaaaatcaa agccagcgat gaaagcgaac tggaatcact gctggatgcg accgcatacg
     1741 aagcattgtt agaagacgag taacggcttt attcctcttc tgcgggagag gatcagggtg
     1801 aggatattta cgcctcaccc tcactctctt cgcaaggaga gaggttcaca attcactgca
     1861 cgtttcagga accatcgctc atgacacaga cgttaagcca gcttgaaaac agcggcgctt
     1921 ttattgaacg ccatatcgga ccggacgccg cgcaacagca agaaatgctg aatgccgttg
     1981 gtgcacaatc gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt cagcttgcga
     2041 ccccgccgca ggttggcgca ccggcgaccg aatacgccgc actggcagaa ctcaaggcta
     2101 ttgccagtcg caataaacgc ttcacgtctt acatcggcat gggttacacc gccgtgcagc
     2161 taccgccggt tatcctgcgt aacatgctgg aaaatccggg ctggtatacc gcgtacactc
     2221 cgtatcaacc tgaagtctcc cagggccgcc ttgaagcact gctcaacttc cagcaggtaa
     2281 cgctggattt gactggactg gatatggcct ctgcttctct tctggacgag gccaccgctg
     2341 ccgccgaagc aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct
     2401 tcgtggcttc cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct
     2461 ttggttttga agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg
     2521 gcgtgctgtt acagcaggta ggcactaccg gtgaaattca cgactacact gcgcttatta
     2581 gcgaactgaa atcacgcaaa attgtggtca gcgttgccgc cgatattatg gcgctggtgc
     2641 tgttaactgc gccgggtaaa cagggcgcgg atattgtttt tggttcggcg caacgcttcg
     2701 gcgtgccgat gggctacggt ggcccacacg cggcattctt tgcggcgaaa gatgaataca
     2761 aacgctcaat gccgggccgt attatcggtg tatcgaaaga tgcagctggc aataccgcgc
     2821 tgcgcatggc gatgcagact cgcgagcaac atatccgccg tgagaaagcg aactccaaca
     2881 tttgtacttc ccaggtactg ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc
     2941 cggttggcct gaaacgtatc gctaaccgca ttcaccgttt gaccgatatc ctggcggcag
     3001 gcctgcaaca aaaaggtctg aagctgcgcc atgcgcacta tttcgacact ctgtgtgtgg
     3061 aagtggccga caaggcgggc gtactggcgc gtgccgaagc ggctgaaatc aacctgcgta
     3121 gcgatatcct gaacgcggtt gggatcaccc ttgatgaaac caccacgcgt gaaaacgtaa
     3181 tgcagctttt cagcgtgctg ctgggcgata accacgggct ggagatcgac acgctggaca
     3241 aagacgtggc tcacgacagc cgctctatcc agcctgcgat gctgcgcgac gacgaaatcc
     3301 tcacccatcc ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc tatatgcact
     3361 cgctggagcg taaagatctg gcgctgaatc aggcgatgat cccgctgggt tcctgcacca
     3421 tgaaactgaa cgccgccgcc gagatgatcc caatcacctg gccggaattt gccgaactgc
     3481 acccgttttg tccgccggaa caggctgaag gttatcagca gatgattgcg cagctggctg
     3541 actggctggt gaaactgacc ggttacgacg ccgtttgtat gcagccgaac tccggcgcac
     3601 agggcgaata cgcgggcctg ctggcgattc gtcattatca tgaaagccgc aacgaagggc
     3661 atcgcgatat ctgcctgatc ccggcttctg cgcacggaac taaccccgct tctgcacata
     3721 tggcaggaat gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc gatctgactg
     3781 atctgcgcgc gaaagcggaa caggcgggcg ataacctctc ctgtatcatg gtgacttatc
     3841 cttctaccca cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt
     3901 tcggcggtca ggtttacctt gatggcgcga acatgaacgc ccaggttggc atcacctcgc
     3961 cgggctttat tggcgcggac gtttcgcacc tcaacctgca taaaactttc tgcattccgc
     4021 acggcggtgg tggtccgggt atgggaccga tcggtgtgaa agcgcatctg gcaccgtttg
     4081 taccgggtca tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc gcagtttctg
     4141 cggcaccgtt cggtagcgcc tctatcctgc caatcagctg gatgtacatc cgcatgatgg
     4201 gcgcagaagg gctgaaaaaa gcaagccagg tggcaatcct caacgccaac tatattgcca
     4261 gccgcctgca ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg
     4321 aatgtattct cgatattcgc ccgctgaaag aagaaaccgg catcagcgag ctggatattg
     4381 ccaagcgcct gatcgactac ggtttccacg cgccgacgat gtcgttcccg gtggcgggta
     4441 cgctgatggt tgaaccgact gaatctgaaa gcaaagtgga actggatcgc tttatcgacg
     4501 cgatgctggc tatccgcgca gaaatcgacc aggttaaagc cggtgtctgg ccgctggaag
     4561 ataacccgct ggtgaacgcg ccgcacattc agaacgaact ggtcgccgag tgggcgcatc
     4621 cgtacagccg tgaagttgca gtattcccgg caggtgtggc agacaaatac tggccgacgg
     4681 tgaaacgtct ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta
     4741 gcgaatacca gtaattcact gattcgacta tcttctaaag gcgcttcggc gcctttttag
     4801 tcagatgaca aagtacaaaa gcgctcagac agtcccctca cccctttggg gagagggtta
     4861 gggtgagggg aacaggccgg cactggcgcg aatatttacc ctcaccccgg ccctctccct
     4921 gaaagggcga ggggaaaagc gtgccaacat tgaagattga gccagtttgt tagcaatctc
     4981 aaagatacgt caacgaatta atttttctcg gaaaaacaaa tggctatagc acttgtgacc
     5041 ggtggcagtc gcggcatcgg gcgggcaact gcattactgt tggcgaaaga agagtatacg
     5101 gtggcggtta attatcaaca aaacctccat gcggcgcagg aagtggtgaa cttaatcacg
     5161 caagccggtg gcaaggcatt cgtgctccag gcggatatca gcgacgaaaa tcaggtcatc
     5221 gcaatgttta cagcaatcga tcagcacgat gaaccgctag cagcgctggt caataacgcc
     5281 gggatcttgt ttacccagtg caccgttgaa aaccttaccg cagagcgaat caaccgagta
     5341 ctttccacca acgtgacggg atattttctc tgctgccgcg aggcggtaaa acgcatggcg
     5401 cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct cttcggtggc ctcacggttg
     5461 ggttcgccag gggaatatgt tgattacgcg gcatcgaaag gggcgattga tacgttaacc
     5521 accggactat cgctggaagt cgccgcgcag gggatccgcg ttaactgcgt gcggccaggg
     5581 tttatttata ccgaaatgca cgccagcggc ggcgagcctg gacgcgtcga tcgcgttaag
     5641 tcgaacatcc ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc cattgtctgg
     5701 ctactaagtg ataaagcctc ttacgtcacg ggaagtttta tcgatttggc gggcgggaaa
     5761 taaaacaggg aagttgtctg accggatgca acaagtattg catccggtac ttcatcgact
     5821 taaagcttct cgccgttgct ggcaatcacc tctttgtacc agttaaagct cttcttacgt
     5881 gaacgcgaca tatcgccagt accgtcgtca tgtttattca catagataaa gccgtagcgt
     5941 ttgctgtact gcccggtagt gaatgacacg cagtcgatgc agccccacgg tgtgtagccc
     6001 atcagatcca caccatcgta agtcactgct tttttcatct cttcaatatg ggcgcgcagg
     6061 tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc gtaagcgcca
     6121 aaaccgtttt cgacaataaa cagcggcctc tgataacgct catacagttc gcaaagggca
     6181 tagcgcagac ctactggatc aatctgccag ccccagtcag acgctttaac atacgggttc
     6241 ggtacgctgc cttcaaaacc agagatcgca tcgcctgtgc cgccttcggc cttcactgcg
     6301 ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcacgcag tacatcgaga
     6361 tcgccgtctt ccattttgat gttaaatccg cgacgctccc actcgttcaa cacataggac
     6421 gggtaatagc cgcgtagctg cacatcggta aagacgtagc gttcgcgcat cgactcctga
     6481 gcgaacatca catcgtccgg gttacagcag tagggataga gcggcaccat cgccagcata
     6541 cagccgactt tcatctccgg gttaatgcga cgcgcagctt tcaccgccag ggcgctggcg
     6601 acaaactggt gatgcagcac ctgatacatc gtctcttccg ggttctcatg ctcggtataa
     6661 accacgccgg agcagcagta accgaacagc ggtgcacgcc agttacgctg gttgttaatt
     6721 tcgttgaagg tcatccagta tttgacttta tgcttatagc gttcaaacac cacttccgcg
     6781 aaacggacaa agaaatcaac cactttacgg ttggtccagc taccgtattg ctgcaccaga
     6841 tgcagcggca tttcaaagtg ggagagggtg atcaccggtt cgatgttgta tttcagcagt
     6901 tcatcgaaca tatcatcgta gaacttcagc ccttcttcgt ttggctgagc ttcatcacct
     6961 tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc catttcggca
     7021 aatagcttga tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg gtttggatag
     7081 tatttgcctg gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc gccaccggtc
     7141 agaacgtcac aaatgctcgg ccctttgccg cctttgttcc agccgccttc gacctgatga
     7201 gcggcaactg cgccgcccca taagaaatct ttcggtaagg tgagtttttt cactatcata
     7261 ggctccagat gagtcaattc ttacaggaaa tagtaccatg cagccaaacg acatcacttt
     7321 ttttcaacgt ttccaggatg acattctggc tgggcgtaaa accatcacca tccgcgacga
     7381 gtctgaatcg cacttcaaaa cgggtgatgt gcttcgtgtc gggcgttttg aagatgacgg
     7441 ttatttttgc acgattgaag tcaccgcaac ctcaaccgta acgctggata cgctgacgga
     7501 aaaacatgca gagcaggaaa atatgaccct gactgaactg ataaaggtca ttgccgacat
     7561 ctatcccggt cagacacagt tttatgtgat tgaatttaaa tgtctttaaa ttgaactcac
     7621 gcctgttagt tgaaatttcg catttaattt catgattttt aggagattag tctcaaatca
     7681 gaatttattt tagctaacag gtgttcactg gaactattct cagttacgct ggagaggtat
     7741 acatgtgcgt gtacgtttct ccggagtaag ttatggttca gaagcccctc attaagcagg
     7801 gatattcgct ggcagaggaa attgccaaca gcgtcagtca cggcattggg ttggtgtttg
     7861 gtatcgttgg gctggtgttg ctactggttc aggcggtgga tctcaatgcc agcgccacag
     7921 cgataaccag ttacagcctc tatggcggca gtatgatcct gctgttcctc gcttcgacgc
     7981 tctatcacgc cattcctcat caacgggcaa aaatgtggct gaagaaattt gaccattgcg
     8041 ctatttacct gttgattgcc ggaacctaca cgccgttttt gctggtgggg ctggattctc
     8101 cgttagcgcg cgggttgatg attgttatct ggagcctggc attgctgggt attctgttta
     8161 aactgaccat cgcgcaccga ttcaaaattt tatctctggt gacctatctg gcgatgggct
     8221 ggctgtcgct ggtggtaatt tatgaaatgg cagttaagct cgcggcgggc agcgttacct
     8281 tactggcggt aggcggcgtg gtttattcgc tcggggtgat tttctacgtc tgcaaacgca
     8341 ttccatacaa ccatgccatc tggcacggct tcgtgctcgg cggtagtgtg tgccactttc
     8401 tggcgatcta tttgtatatt gggcaggcgt aatgggtatg cctttgtagg cctgataaga
     8461 cgcgccagcg tcgcatcagg cgataaatgc ctgatgcgat aaagagattt actcttcgag
     8521 cgaatacggc agcggctcga tatgcagtgt attcgcatcg tcgcgtacgc ggaagatgct
     8581 atccggttcc atatcgttat tcatgacgac ctgtaccacg acctgaccat cttccagttt
     8641 taccgcagcc agcaccgtac cggtacgacg ccagttctcg cccattttca gctctaagtc
     8701 ttcaccagct tccggcagtc ggctggcgct acctgccagc aaccagagcg cacgtttatt
     8761 ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtatagcagc cttttttaaa
     8821 gctgataccg cccagcgcct ggaggttggt cgcctgtggg ataaactgcc cgctgttggc
     8881 ggcatcaatc accgggaaac ccgcttcaat gtttaatgcc agccactgtt ggctattgtt
     8941 cagttccgct tcaccgcgca gtttatcggt cagcatatta gcagtagctt catcggttac
     9001 gatcaggaaa cgttctgccg ggtgttcaaa ccatagcaaa gtggtcgcgc cttctttaac
     9061 tacctgtttt tctttcgaag gcagttcact aaagagattt gccagcgcgg cgcgcgcctg
     9121 aaaaccggca acaccaagca gcacacgctc gtcgtctggc gcgatggtca ctttagagaa
     9181 taccgcatat tttttcagtt cagtcagctg cggttcacgc acgctgcgcc gttcaatcca
     9241 tgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttac ctttggcgtc
     9301 gcaatgggcg gcgagcaggt gctgatcttc tgccatctgg ctgacatctg ctgtcacctg
     9361 accctgcata tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc agtcatcaag
     9421 cgtcatcagc gtcagcggta aacgggcaga agccgtaggc tgacggggag gaaaaggtgt
     9481 aaaagccata atagagtcct gagttgctta acggcgtatt aatgactaat ggtaaaagag
     9541 ctatgaggca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag gcgacttcca
     9601 aaaaaatgaa gccattgaat tgttgtgaat taatttcgcg atcgcgatgg tgtttactga
     9661 tattcccgta aaagaaggcg aaaaacacgt tacaatagcg gggtactgga ttttcgtgag
     9721 acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc cgccgtggta
     9781 tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtacgac agcttaagcg
     9841 atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatccggac ctgtttaact
     9901 ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc cgactcatcc
     9961 agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc ctggcgcgca
    10021 cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact catgccctgg
    10081 ccactcagtt acaccccgtt atggatggtg ttactttcgc tggtggtgtt tgattgcgtt
    10141 cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat ggacgggcgt
    10201 ttgcgttggc aagggcagga gtggagcatc gtcaaagcac cgtggatgat taagagcggc
    10261 atgatgctgc gtttacgttc tgatggcggt aaacggcaac atttatggct ggcagccgac
    10321 agcatggacg aagccgaatg gcgggattta cggcggattt tgttgcaaca agagacgcaa
    10381 agataaagat gacggcaaag catatgcgct aagccgtcag tgagatcagg cgtaatgctc
    10441 tgccatttcg ttgaggattt gctcgcacca gctctgaata cgctcgtcgc taaggtcata
    10501 ctggttagtt tcatccagcg ccagacccac gaacagttgc ccgtcagcaa tcaccggttt
    10561 cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca cgcctttggt
    10621 cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt atcccagttg
    10681 atcgccaagc ccatacagcg caacaatttt accttcaagg ttcaggtcgt cgagctgatc
    10741 ccagacggct tcccagtctt cttggatttc accaaaatcc caggtcggga tacccagaat
    10801 gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat gtaaggtcac
    10861 cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt aacaggtgct
    10921 ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct gtggcttttg
    10981 cggctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg atcaaatgag
    11041 tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt tcttgatgct
    11101 ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg cgatctgtca
    11161 atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca aagtgacgat
    11221 ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag ctcagcgcgt
    11281 ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt tcgtgaagac
    11341 gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa agatttaagc
    11401 gaagcgcagg ttgaacgttt attacaggca ccattaattg atcagccact ggagctacgc
    11461 gataaagcca tgcttgaagt gttgtatgct accgggctgc gtgtctctga actggtcgga
    11521 ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg taaaggcaac
    11581 aaagagcgcc tggtaccgtt aggtgaagag gcggtttact ggctggaaac ctatctggaa
    11641 catgggcgtc cgtggctgct gaatggtgtg tcaattgacg tgttgtttcc cagccagcgt
    11701 gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc tgtgctggcg
    11761 ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc cacccattta
    11821 ttaaatcatg gtgcggattt acgcgtggtg cagatgctac tgggccacag cgacctctcc
    11881 accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca tcaacagcat
    11941 cacccgcggg cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg ttttatgttg
    12001 tttactttgt tagcggcgtt ttcaggtttt gctcaggctg atgacgcggc aattcaacaa
    12061 acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt agctggcatg
    12121 aagacagttc tgactaacag cggcgtgttg tacatcaccg atgatggtaa acatatcatt
    12181 caggggccaa tgtatgacgt tagcggcacg gctccggtca atgtcaccaa taagatgctg
    12241 ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgcc gcaggaaaaa
    12301 cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact gcatgagcaa
    12361 atggcagact ataacgcgct ggggatcacc gtgcgttatc ttgctttccc gcgccagggg
    12421 ctggacagcg atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga taaaaacaaa
    12481 gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga cgtggatatt
    12541 gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc agttgtgctg
    12601 agcaatggca cacttgttcc gggttaccag ccgccgaaag agatgaaaga attcctcgac
    12661 gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa acaacagata caacttcgtc
    12721 gccgtgaagt cgatgaaacg gcagacttgc ccgctgaatt gcctcccttg ctgcgccgtt
    12781 tatacgccag ccggggcgtg cgcagtgcgc aagaactgga acgcagtgtt aaaggtatgt
    12841 tgccctggca gcaactgagc ggcgtcgaaa aggccgttga gatcctttac aacgcttttc
    12901 gcgaaggaac gcggattatt gtggtcggcg attttgacgc cgacggcgcg accagcacgg
    12961 ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa tatcgactat ctggtaccaa
    13021 accgtttcga agacggttac ggcttaagcc cggaagtagt cgatcaggcc catgcccgtg
    13081 gcgcgcagtt aattgtcacg gtggataacg gtatttcctc ccatgcgggc gttgaacacg
    13141 ctcgctcgtt gggcattccg gttattgtta ccgatcacca tttgccgggc gaaacattac
    13201 ccgcagcgga agcgatcatt aaccctaact tgcgcgactg taatttcccg tcgaaatcac
    13261 tggcaggcgt gggtgtggcg ttttatctga tgctggcgct gcgcaccttt ttgcgcgatc
    13321 agggctggtt tgatgagcgt ggcatcgcaa ttcctaacct ggcagaactg ctggatctgg
    13381 tagcgctggg gacagtagcg gacgtcgtgc cgctggacgc taataatcgc attctgacct
    13441 ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc ggggattaaa gcgctgctgg
    13501 aagtggcaaa tcgcgatgcg caaaaactcg ccgccagcga tttaggtttt gcgctggggc
    13561 cacgtctcaa tgctgccgga cgactggacg atatgtccgt cggtgtggcg ctcttgctgt
    13621 gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta aaccagacgc
    13681 gaaaagagat cgaacaagga atgcaagttg aagccctgac cctgtgcgag aaactggagc
    13741 gaagtcgcga cacgctaccc ggcgggctgg caatgtatca ccccgaatgg catcagggcg
    13801 ttgtcggtat tctggcttcg cgcatcaaag agcgttttca ccgtccggtt atcgcctttg
    13861 cgccagcagg tgatggtacg ctgaaaggtt caggtcgctc cattcagggg ctgcatatgc
    13921 gtgatgcact ggagcgatta gacacactct accctggcat gatactgaag tttggcggtc
    13981 atgcgatggc ggcgggtttg tcgctggaag aggataaatt cgaactcttt caacaacggt
    14041 ttggcgaact ggttacagag tggctggacc cttcgctatt gcaaggcgaa gtggtgtcag
    14101 acggtccgtt aagcccggcc gaaatgacca tggaagtggc gcagttgctg cgcgatgctg
    14161 gcccgtgggg gcagatgttc ccggagccgc tgtttgacgg tcatttccgt ctgctgcaac
    14221 agcggctggt gggcgaacgt catttgaagg tgatggtcga accggtcggc ggcggtccac
    14281 tgctggatgg tattgctttt aatgtcgata ccgccctctg gccggataac ggcgtgcgtg
    14341 aagtgcaact ggcttataag ctcgatatca acgagtttcg cggcaaccgc agcctgcaaa
    14401 ttatcatcga caatatctgg ccaatttagc gtcatcttct ctataaaaaa gagcgtggat
    14461 tgggtacaat cccgctctta tcaccgcatt ttgactagct caataaaaga aatcagacca
    14521 tgtttgaaat taatccggta aataatcgca ttcaggacct cacggaacgc tccgacgttc
    14581 ttagggggta tctttgacta cgacgccaag aaagagcgtc tggaagaagt aaacgccgag
    14641 ctggaacagc cggatgtctg gaacgaaccc gaacgcgcac aggcgctggg taaagagcgt
    14701 tcctccctcg aagccgttgt cgacaccctc gaccaaatga aacaggggct ggaagatgtt
    14761 tctggtctgc tggaactggc tgtagaagct gacgacgaag aaacctttaa cgaagccgtt
    14821 gctgaactcg acgccctgga agaaaaactg gcgcagcttg agttccgccg tatgttctct
    14881 ggcgaatatg acagcgccga ctgctacctc gatattcagg cggggtctgg cggtacggaa
    14941 gcacaggact gggcgagcat gcttgagcgt atgtatctgc gctgggctga atcgcgtggt
    15001 ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa atccgtgacg
    15061 atcaaaatct ccggcgatta cgcttacggc tggctgcgta cagaaactgg cgttcaccgc
    15121 ctggtgcgta agagcccgtt tgactccggc ggtcgtcgcc acacatcgtt cagctctgcg
    15181 tttgtttacc cggaagttga tgatgatatt gatatcgaaa tcaacccggc ggatttgcgc
    15241 attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac cgaatctgcg
    15301 gtgcgtatta cccacatccc aaccgggatc gtgacccaat gccagaacga ccgttcccag
    15361 cacaagaaca aagaccaggc catgaagcag atgaaagcga agctttatga actggagatg
    15421 cagaagaaaa atgccgagaa acaggcgatg gaagataaca aatccgacat cggctggggc
    15481 agccagattc gttcttatgt ccttgatgac tcccgcatta aagatctgcg taccggggta
    15541 gaaacccgca acacgcaggc cgtgctggac ggcagcctgg atcaatttat cgaagcaagt
    15601 ttgaaagcag ggttatgagg aaccaacatg tctgaacaac acgcacaggg cgctgacgcg
    15661 gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa cctgcgcgag
    15721 caggggattg ccttcccgaa cgatttccgt cgcgatcata cctctgacca attgcacgca
    15781 gaattcgacg gtaaagagaa cgaagaactg gaagcgctga acatcgaagt cgccgttgct
    15841 ggccgcatga tgacccgccg tattatgggt aaagcgtctt tcgttaccct gcaggatgtg
    15901 ggtggccgca ttcagctgta cgttgcccgt gacgatctgc cggaaggcgt ttacaacgag
    15961 cagttcaaaa aatgggacct cggtgacatc ctcggcgcga aaggtaaact gttcaaaacc
    16021 aaaaccggcg aactgtctat ccactgtacc gagctgcgtc tgctgaccaa agcactgcgt
    16081 ccgctgccgg ataaattcca cggcttgcag gatcaggaag cgcgctatcg tcagcgttat
    16141 ctggatctca tctctaacga tgaatcccgt aacaccttta aagtgcgctc gcagatcctc
    16201 tctggtattc gccagttcat ggtgaaccgc ggctttatgg aagttgaaac gccgatgatg
    16261 caggtgatcc ctggcggtgc cgctgcgcgt ccgtttatca cccaccataa cgcgctggat
    16321 ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca agcgtctggt ggtcggtggc
    16381 ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg aaggtatctc cgtacgtcat
    16441 aacccagagt tcaccatgat ggaactctac atggcttacg cagattacaa agatctgatc
    16501 gagctgaccg aatcgctgtt ccgtactctg gcacaggata ttctcggtaa gacggaagtg
    16561 acctatggcg acgtgacgct ggacttcggt aaaccgttcg aaaaactgac catgcgtgaa
    16621 gcgatcaaga aatatcgccc ggaaaccgac atggcggatc tggataactt cgactctgcg
    16681 aaagcgattg ctgaatctat cggtatccac gttgagaaga gctggggtct gggccgtatc
    16741 gttaccgaga tcttcgaaga agtggcagaa gcacatctga ttcagccgac cttcattact
    16801 gaatatccgg cagaagtttc tccgctggcg cgtcgtaacg acattaaccc ggaaatcaca
    16861 gaccgctttg agttcttcat cggtggtcgt gaaatcggta acggctttag cgagctgaat
    16921 gacgcggaag atcaggcgca acgcttcctg gatcaggttg ccgcgaaaga cgcaggtgac
    16981 gacgaagcga tgttctacga tgaagattac gttaccgcac tggaacatgg cttaccgccg
    17041 acagcaggtc tggggattgg catcgaccgt atggtaatgc tgttcaccaa cagccatacc
    17101 atccgcgacg ttattctgtt cccggcgatg cgtccggtga aataagcatt acgttatgct
    17161 cacaaccccg gcaaatgtcg gggtttttta tttaagctgg gtaaatgcag ataatcgtat
    17221 tctggcttcg cgatttgtcg cctgcatcac catccacgga ctgaacgccc acggcgtggc
    17281 atcaataccg tgtaatacat cagctaaatc acaccattga taatccatca cttcatcatc
    17341 gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt cattttccac
    17401 aatgccactc ggatcggtgg cgcggtagcg aaagtcagga tagatagatt caggaggcgt
    17461 aatttccacg ccaagctcat aacggcaacg gcggatcacc gcgtcttcgc tgctttctcc
    17521 cagttgtggg tgcccacaaa ccgagttagt ccacacgcca ggccacgctt ttttgctcag
    17581 tgcgcggcgg gtaactaata attgcccctt ggcattaaac agccaactgg agaacgcgag
    17641 atgtaagagg gtgtctgccg tgtgtgcggc atacttttcc agcgtaccag tgggaactcc
    17701 ctgtgcattc aataaaatga cgtgttccgt ttgcataatt tctcacatgt aattctgatc
    17761 actcatcatt atagataatt gaagagtgaa atgattaacc aagtgagcgt ttatcgacaa
    17821 ccgcccgttt tgagcggatg ccgacaggta aaatccattt aaaaaagtag catcatatat
    17881 ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat agagagagta
    17941 aaaagcaaca gcagactgaa tatagcgcgc attaaaaaat tcatattatt ctccatgctc
    18001 atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt ttaaatcttt
    18061 atcagagacg gattccttca ccaggtcagc atgatgctgg tagccattaa agaagacatt
    18121 cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgcagcaacg gttgcagtac
    18181 ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa cgccgagact
    18241 gatcgccaca atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg acagaattcg
    18301 aatccctgtc gccagtacca tgccgaacat caccagccca gcaccgccca gcacaaattg
    18361 cggaatggag gcgaccagca ccgccatttt tggcaccatg ccaaacagga ttaatataat
    18421 tcccgaagaa atacacaccc agcggctatg aacgcgcgtc acgctaacca ggccaacgtt
    18481 ttgtgaaaat gacgtgtggg ggaagctgtt aaacgttccg cctatcattg tccctacgcc
    18541 atcgacacgc agcccgcgaa taatatcgtg tgaagagagt ttacgaccga ctatttcacc
    18601 cagtgccagg aacatcccca tcgactcgat aaacacgatg attaacacgg cagtcatggt
    18661 cagaatggaa acgggatcga aaatcggcat accaaatgac atcggcgtga caatcgcaaa
    18721 ccatgaagca tcatgtagcc cggataaatt gacttcattc atcatccacg aaagtaaaaa
    18781 gccaaataca atccccagta atacggcgac gttggacata aatcctttcg catagcgagt
    18841 aatgagcaag ataaaaatta agacagcaaa ggagatacct aaataaacgg gattaccata
    18901 ttgcggattc cctttacctc cggcggccca gtcaataccc acctgaatga tgctaagccc
    18961 aatagaagta ataaccacac cggtaaccag tggcgggaat aaaggcatca agcgaccgat
    19021 aagtggcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa atatccccag
    19081 caggccgata tccgggttca tcccaatggc tatcattggt gttacagcag caaaggtcac
    19141 cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac attgcaataa
    19201 tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag catcttttga
    19261 gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt acatgacaag
    19321 gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt caacctcatc
    19381 agttgggcgg tcttgcccag aagatgaggg aagttgggaa tctatggcgc tcatcagtgt
    19441 ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac tgtctaaaca
    19501 catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga attgcggagg
    19561 caatcacata atcgtgctta aaccacgaaa atgacaagag aacctgacaa tggaattatc
    19621 tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact ctcgcattta
    19681 atcgttttta tctggatagc gctcttttgg tcggcaatgt tgaaataatg aaagcggcat
    19741 agattgaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg gtcatgctac
    19801 ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca ttaaatgaaa
    19861 gggatgcaaa tgaataagtt tatcgctgct gaagctgcgg aatgtatagg ctgtcatgct
    19921 tgtgaaattg cctgtgcggt ggcacacaat caagaaaact ggccgctgag tcacagtgac
    19981 tttcgaccgc gtatccacgt tgtagggaaa ggccaggctg cgaatccggt ggcctgccat
    20041 cactgcaaca atgccccttg cgttacggct tgtccggtta atgctctgac tttccagtcc
    20101 gatagcgtac aactggacga gcaaaaatgt attggctgta aaagatgcgc aatcgcttgc
    20161 ccctttggcg tcgttgagat ggtcgatacg attgcgcaga aatgcgacct ttgtaaccag
    20221 cgcagttctg gcacgcaagc ctgtattgaa gtctgcccaa cacaggcgtt acgactgatg
    20281 gacgataaag ggttacagca gataaaggtg gcccgccagc gcaaaacggc agcaggaaaa
    20341 gcgtcatcag tcgctcagcc atctcgcagt gcagcgttgc tccccgttaa ctcgcgtaaa
    20401 ggcgcagata aaatttcagc aagtgaacgg aaaaaccact ttggcgaaat ctattgcggg
    20461 ctggatccac tgcaagcgac ttatgagagt gaccgctgtg tttattgtgc cgaaaaagct
    20521 aactgcaact ggcattgccc gctgcataac gctattccgg attacatccg tctggtacag
    20581 gagggaaaga ttattgaagc ggcagaactt tgccaccaga ccagttcctt accagaaatc
    20641 tgcggcaggg tatgtccaca ggaccgtctt tgtgaaggcg catgtacttt gaaagatcac
    20701 tctggcgcag tcactatcgg taatctggaa cgctacatca ccgataccgc gctggcgatg
    20761 ggctggcgtc ccgatgtcag caaagttgtt ccccgtagcg aaaaagtggc ggtgattggc
    20821 gctgggccag cagggttagg gtgtgctgat attctggcgc gcgcaggagt tcaggttgat
    20881 gtctttgatc gccatccaga aattggcggt atgctgactt ttggcattcc tcctttcaaa
    20941 ctcgataaaa cggtattaag ccagcggcga gagatattca ccgcaatggg aatcgacttt
    21001 catctcaact gtgaaattgg ccgcgatatt acctttagca atttaacttc tgaatatgat
    21061 gcagttttca tcggcgtggg gacttacggg atgatgcgag cagatctgcc gcatgaagat
    21121 gcgcccggtg tcattcaggc tctaccgttc ctgactgccc atacccgcca gctcatggga
    21181 ttgccggagt ctgaagagta tccgctgacg gacgtggaag gtaagcgagt cgtggtattg
    21241 ggcggtggcg atacgacaat ggattgtttg cggacttcca tccgcctcaa tgccgccagc
    21301 gtgacctgcg cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa agaggtggtt
    21361 aatgcgcgcg aggaaggtgt cgagtttcag ttcaatgttc aaccgcaata tatcgcttgt
    21421 gacgaagatg ggcgtttaac tgcggtgggc ctgattcgta ccgccatggg tgagccgggg
    21481 ccggatggtc gccgtcgtcc tcgtccggta gcgggttcag agtttgaatt gcccgccgat
    21541 gttctgatta tggcctttgg tttccaggcg catgccatgc cgtggttgca gggcagcgga
    21601 attaaactcg ataaatgggg cctgattcaa accggtgacg tcgggtattt acctacccag
    21661 acgcatctga aaaaagtctt tgctggtggt gatgcagttc atggcgcgga tctggttgtc
    21721 actgcaatgg ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt tgatacgaag
    21781 gcatcgtgat gaaatcgtta attatcgtta atccggctga ctgtattggc tgccgtactt
    21841 gtgaagtagc ctgtgtggtc gctcaccctt cagaacagga gttgaatgcc gatgtctttt
    21901 taccccggct gaaggtacag cgactggaca gcatcagtgc gccggtgatg tgccatcagt
    21961 gtgaaaacgc cccttgtgtt ggcgcttgcc ccgtgggggc gctgacgatg ggcgagcagg
    22021 tggtgcagac caattctgcc cgttgtattg gctgtcagag ttgcgtcagt gcgtgcccgt
    22081 ttggaatgat caccattcag tcgttgccgg gggatacccg acaacaaatt gtgaaatgcg
    22141 atctctgtga acagcgggaa gaggggccag cctgtgttga atcttgcccg acacaggcgc
    22201 tgcagttgct tactgaaaga gaactcaggc gagtccgcca gcagcgtatt gttgccagcg
    22261 gtgagaatcc tctgtgaaag aaatctccgc gctgaaaggc gcggagaatc tattagattg
    22321 cccaaccacc cgcgtaaaat gcgaccagtg cggcagtaat gatcaccgta ccaatattca
    22381 gcttttgcca ttcgcgtgca aagacgcggc ctacgaccag tgtcacaaag cccagcataa
    22441 taccggtaac gatattacaa gtcagaacga tgaacacggc acacaccagg ccagccatag
    22501 cgtcaataaa atcattgaaa tccagcttcg agacgttact taacatcagc aaacccacgt
    22561 acatcagagc gggtgcagtg gcgtaaccag ggatcaaaaa tgacagcggt gataagaaca
    22621 gaatcagcag gaataacgcc cccactacgg ttgcggtcaa ccctgtttta cccccggcgg
    22681 cggttcctgc cgctgattcg atataaaccg ctgcgggcgc tgcgcccacc agaccggaga
    22741 atattgaact tactgagtca ctggtcaggg ctttgccgcc gttgatgatc tggttgtctt
    22801 tatccaacaa attcgcctga ccggcgacgg cacggatggt gccagtggcg tcgaacactg
    22861 cggtcatcac caatgccagt acactcggaa gtacagttgg ctggagtgcg cccataatat
    22921 cgaggctgaa aatcagagac ttaccatctt cgccagtcag gcttggcatc gccaccagac
    22981 cgtggtattt caccgctggg tcaaagatta agccgatgat cgaaattgca ataatcacca
    23041 acaagatccc gccgggtacg cgacacttct ccagaccgaa gatgaccgcc agccccagta
    23101 agctcatcat caccgggaag gaggtaaacg caccgagcgc cactggcaag ccttcaatcg
    23161 ggtttttgat aaccattccc acaccgttag cagcaatcag cagcagaaac aggccgatac
    23221 cgatacctgt accgtgagcg atccccatcg gtaaattacg taagatccag gtacgcacac
    23281 cggttacgga aatagcggtg aagatgacgc ccatcagaaa gaccgcgccc agtgcgacag
    23341 gaacgctaat ttgttgcccg agtaccagac tgaatgcggt aaacgccgtc aaggaaatcg
    23401 cgcaaccaat cgccattggc aaattggccc ataatcccat cagcaacgag ccgaagcccg
    23461 cgaccagaca ggtggcaaca aacacagctg cgggaggaaa acctgctttg cccagcattc
    23521 ccggaacgac gataacggaa taaaccatgg ccagaaaggt cgttaagcca gccagtactt
    23581 cctgacgaac ggtactgcca cgagcggtaa ttttaaaata attatccagc gcgccctgtg
    23641 gctttggtgc gtccggtgtt tgtaggatgt ctccagacat aatgatgtcc tcacgaggat
    23701 gttaaagttt tattattagt tgcgttcgta caccagacga ccatcaacgt aggtgcggta
    23761 gatcgaacgg tcatcgccca acgtcatcat cacgaacaat ttgtcgacta aagaaacaga
    23821 gttgtcatag cgcagctgct gtagcggagt ggcggtgggt tccatcacca cgaaatcagc
    23881 ctctttgcca ggtaaaaagt tgccaatcaa atcgtcaagg cccagagatt tcgctccgcc
    23941 gagcgtggcc aggtaaaacg cttcatatgc cgagaggcga tagccttgta attgcaacac
    24001 tttgtaggct tcgttcagcg tttgcagcat gttgaaagtg gttccggcac cgatatccgt
    24061 tcccatgccc actttaactt tcttctgcca tgcttttttc aagttgaata agccgctgcc
    24121 gaggtaaagg ttggaggtcg gacagaaagc aatgctggat ttggtttcgc tgagacgatc
    24181 ccactctttt tcttcgagat ggacgcagtg agcaaagaca cagtttttac cggtcaggcc
    24241 gtactgatgg taaacatcca gataaccatc atggtcagga taaagcgatt tcacccaggc
    24301 aatttcatct ttgttttcac agagatgggt atgtacccac gtatccggat attcttcttt
    24361 caggcgttgc gccatcgcca tctgttcagg agatgaggtc ggggcgaagc gtggcgtaat
    24421 cgcatatagc agacgaccat ttttgtgcca gcgttcaatc agttctttgc tttggtgata
    24481 gctgctttcg gcagtgtcga gcagataatc cggtgcgtta cggtccatca tcaccttacc
    24541 ggcaatcata cgcatattga tatgactggc ggcttcaaac agcgcatcaa cggattgcgg
    24601 atgaacagtg ccaaacacca gcgccgtggt ggttccgtta cgtaaaagct gcttgatgaa
    24661 gaacgccgac atttcgcggg cgtactctaa atcctcataa cgccgttcag tagggaaggt
    24721 gtgtttattc aaccactcca gcaattgctc accataggcc cccaccattt cactttgcgg
    24781 ataatggata tgtgtatcga caaagcccgg tactatcagt ttgccgcgat agtcgcgcac
    24841 gcgaatggtg tcaggaattt gatgctttcc gttttcccat tcgccaaacc attccacttt
    24901 tccctgttta atgagtaata aaccatcctc aataaaccgc agcgcagagg caatctcttc
    24961 cgggttatcg accgtacggg tgacatcaat aaaactgcct cgtaccgctt ttaacgtgtg
    25021 ttctcctgac atcattgact cctttactgt taatccatct cttccgctga ggtaatacca
    25081 ggcagaacgt tttcctgtcg gtagccacca gggaggataa tatttaataa aatcgcagtt
    25141 aacccgccag cacaaatagg gttttcaact aatacataaa tagaggctgg caatatttta
    25201 aaaatttcgg gatcgtagga gacgccaagc cctaaaccta aagaagtggc gacaataagt
    25261 gtttcacggc gctttaaacc gttggtgatg atgatgcgaa tccctgcgat ggcaatcatg
    25321 gaaaacatca acgtcattgc gcctcccaga actgccgagg gaatggtcgt gaagaagccg
    25381 ccaatcatcg gaaataagcc gaggataacc agcattaccg cgatggttcg cccgacataa
    25441 cgtgaagcga cgccagtcat ctgaataacc ccattatttt gcgcaaacgt ggttaatggt
    25501 aatgaaccga cagcggaggc gataacagaa accagaccat ctgccagcac gccgcctttc
    25561 agtcgggact gatactcttc cccctgaatg gggcggcggg aaaccattgc cgtggcggtg
    25621 atatcgccga ctgcttccag cacgctaagc agataaatcg tgccaaccac caggaactga
    25681 tggaagctaa aactaaagcc gtatttgaat ggatgtggga tggtgattaa cggcaaattg
    25741 cgcatactgc tgaaatccac catgcccagg cataacgatg cgatatagcc gacacatagc
    25801 ccaatagcga tccctcccat gcgtagcaac ggactgcgac agcagttaaa gccgatcacc
    25861 acaatcaaaa ccaataaacc aacgccgaga tgttcgtaat tgccgaaggt accgctgctt
    25921 ttggcagcaa atcctccacc aaaatcaata atgccgactt taatcaggct taagccgatc
    25981 atcagtacca caataccgct gacagtaggc gtaataaccc gacgtaaata gggcaggata
    26041 aaagaagagc cgacaaccag aaatgcgcca acgaaggaga cgccgagaag cgacgacatg
    26101 attaactctt cgtgaaaacc gtcgcttttc atgctgctgc ccagcgcaat catgaccgta
    26161 acaaatgaaa aattgactga ctgaattgag agtaggccag aaccgacgat gccgtagcgg
    26221 tttacttgta accaggtacc aataccagag gcgatcatcg ccattgaaac aagataggca
    26281 gttgtttcag cggaaagctg taaggccgca cccacgatta acgcgggggt taccatcgga
    26341 acgaaaattg ccaacagatg ggtaatggca ccaacgagag cctgatgaaa gggagggcga
    26401 tcctccagtt caaatataag gtcagaacct gcatggttta tatcagacat cccttcactc
    26461 cttttaaagt aaatttgatc atctgaatta gtaaaataaa ttcccacgaa tttggggcgc
    26521 aggacgcctt gagaacggct tatctcattt tcgcagtcac tatttagtca tcgttcaaac
    26581 agagaaacag gccgccgaag cggccattta ttacttatat tttttccagc gcggtgagta
    26641 ttttctccgg tgtgaaatgc cattcgcgta accagatgcc gcaggcatcg tgaattgcgg
    26701 tagcaatcgc cggagctgcg ccatttacac cgatttccga gatcgatttc gccccgaacg
    26761 ggccgacttt atcgtcgctc ggcaccagca cagcgcggaa atcacgcgga atgtcaccaa
    26821 ttttcggtgc gccgtaactg cgtaaatcac gcgttaacgg gtgaccttcg gcgtcataaa
    26881 tgatctcttc gctcatactg tggccgatag ctcgcagggt agcaccgtag atttgtccca
    26941 atgctaattc cgggttgacc ggtgtaccgc agtccagcaa ggcgtagaat ttatccaggc
    27001 ggatttcacc cgtacgcgtg ttgacggcaa cttcagcgaa gtttgcgcca tacgggaagg
    27061 cgaaatcagg cgtgatataa ctgccagttc ccaccagtga accaaagccg gtgccggttt
    27121 cgcctttatg agcaatatcc ccgaagctga cttcgccttt cttgccgcgc acgacgcccg
    27181 gcgttgccag ttgaacatct gccactgcct cacccaacat ttgcgcgccg tggaacagaa
    27241 ttttctcccg cagattttcc gctgccaaac gcgccgcgtt acctgagaag caagtaccgg
    27301 acgaggcata tgcgccttta tcaaacaacg catgatcggt atcaccggag ataacatgca
    27361 cgtcctgcgg tgggcaatgc agcacttctg ctgccagttt cgtcaccaca gtatccagac
    27421 cagtaccaat atccgcaccg ccagaatgaa cgataaaggt gccgtccgat tccagtttga
    27481 tcatgcagtt agcctgatcg atatccggga tacccgattt ctgcatgata atggcgacgc
    27541 cgcgaccgat gtgccagtca ccattttgcg gtttcggtga cgaccattgg atcatctcgc
    27601 gaccctgacg caggatctct tctagtgcac agctggcggc ggaaggaacg gaggtcggcg
    27661 ctttaccttc accgattgca ccgagaattt tcagctcttg cccttcgtgt acccggttac
    27721 gttcgataat ttccagttgg tcgatctgta actgttcagc cagttccgct aatgccatgg
    27781 tgatagcgaa gttacctttc ggtgcgccat aaccctgata agcaccattt gggcaaatgt
    27841 tgctgtagta ggtggtgacc tgaaaatcga cgttatcgca cggatataac ggcagcgaca
    27901 gcgccggtcc gttacatggt acggtgagtg agtggttgcc gtaagggcca gtgttggcgc
    27961 ggaaatccat cttcactgct gtcaggcgac catctctttt cgctcccagt ttgacggtga
    28021 ctttcgcgac gtgacgagag gtgttagcaa taaactcttc ttcacgggtg tagcggaaca
    28081 gtaccggacg cccggtcacg caggttgccc aggcgcacac ttcttccagc aggatgtcct
    28141 gtttggaacc aaaaccgccg ccgactcgct ctttaatgac atgaacttta tgctgtttca
    28201 tgcccacgag gcgcgcgacc tggcggcgta agtgccatgg tacctgggtg gaggcgtgga
    28261 taaccagacg atcgccgtcc atacgagtaa agcagatatg tgtttcagtc gggcactgct
    28321 gcgcttgcgt tgagttatag gttcgctcaa tgatcacatc ggcatcggca aagcctttgt
    28381 ccatatcgcc aatatgacca tgaatactgg cggcgatatt tttgcggggg cgagaaccga
    28441 tcgggaagtt aatgatcata tgctcgccgc gctgggctgc atggctgtta tcgtcttcca
    28501 gagtatctgg cgcaccagca acatacacca ccggttcatc gtgcacgaca ggcgcatctt
    28561 ccgccattgc ttcgtcgatc gacattaccg gcttaagcac ttcatattca acgtcgatga
    28621 gcttcagtgc ttcgagcgca atttcttcac tttctgcgac caccgcagca acacgatcgc
    28681 cgacgtgacg cattttcttg ccgaacatac ggcggtcaag cggtgacggt tccggtgcgc
    28741 tctgaccccc cggggtatag tagatatccg ggcaattcag gtgagtaata acgtgaacga
    28801 cgcccggtaa ggcttcagct ttgctgacat ccagatgagt aatcagtgcg tgagcgtgtg
    28861 ggctacgtaa cattttaatg acgcaggcgt cagccgttac gcggtcttca acatagcagg
    28921 gtttcgcctg caccattttc gcggcatcag ttttaggata atgcttgcca atgacttcaa
    28981 ggtcgtcacg gaaagtcgga gcgatatcaa tggtggcctg cggattattt ttacgtgcaa
    29041 ccgccagttc aatgacctga tagtattgtt gccagcctgc atcgcggctg aacaaaccag
    29101 aaagcgcgtc gtcgatctct tcgcgggtag gtgcggcgat gcgatcgaga agatccgtga
    29161 taatcagagc tgcagctgga tcgttataac cagactgcac cacgccaaca tcaaccattg
    29221 cctgttgaac cagacttaac tcgttccatt tgcccagaga ttctgcggta cgaatatctg
    29281 ccttctctaa ctgtgcggca ataagcaagg acgcgttaac gatattaccg ttaaagatta
    29341 ttgcgtcaga accggcaaac ccgaagccat catcactgtt gcgtacagag tgcattccca
    29401 tgttaaacaa cagcttttgc acgttttcgc ctggattaac ggttagctcc tgaggcgcgc
    29461 catttaaagt aaagtggatg atcatacggc ttcctcccca gcctgttggc agtcggcata
    29521 caggtcggct accagaactc ccgtgatata gcgtttatag gccacgctgc cccgcaaatc
    29581 ttcctgcggg aaaatggcgt tggcgacagc ctgttcaagt gcattgcctt ccagattttg
    29641 tttttcgaca tcgtgcagac gcagtgcttt actggcgacg ccatccagcg caattcgcat
    29701 accgtcgtgg tctgtcattg caacggctgc cgtcacgacg gttaaacctg cctgagaacg
    29761 gctaattttg cgggtcgcac aggtgcgata cggatcttta atgataattt cggttagcag
    29821 gcgatcgcat gggctggcca ggtagtcctc gattgacagc gtttcgccgt tgccaaaaac
    29881 cagttcagca tccagtgcca gcaggacagg aagcagcacc gactcttcct gccgggcggc
    29941 gatttcacca ccaatggtcg actgattacg aacatggcgt gagtaaacaa aaccgagggc
    30001 ttcacgcagt gctgcaggaa taaatcgcgc atcacgcagt ggctgcaagc gagacattgc
    30061 accaatccgc agtgcaccgt tatcccagtc aacccagtcc agttccagat cctgtaagga
    30121 aatggcaatc tttttatcgg tacgggttgg tgtagcgttg agtttgctgc ccccggcgaa
    30181 ccagacggct tcatcctggt agcggcgctt cagttccagc gcctgttcga cagagtcggg
    30241 cctgaaaaat tgttcaatca tcttctttcc ttatttaacc ctcttccctg cgggaagagg
    30301 gcatctgtca tttatgccag cgtatccatc cgacgccaca tactggcagc ggcttttctg
    30361 gcttgtgcat aaatggaatc gcaatcaaag ttaaactgac ggtcttcata gaccatcacg
    30421 ccattgacca tcacgctgtg aacgctgcct gagcccatac cgaaagcgat atgcccggcg
    30481 atattgtctg ccagcagcgg cgtcggcgag ttgtaatcgc aaatggttaa atcggctttg
    30541 taaccggctt ccagaagacc aaatttcgcg gcaaagttgc ggctcatcag ttcgttaccg
    30601 ttagtcaggg ctttggcaaa actgtcaggc cacagcggac cacccgcatc gcgatgttta
    30661 aagaaggcaa atttcatctc ttcaaacatg tccgaaccaa tgccgtccgt tcccaacgcc
    30721 agattgcgga tgtcgctaag gtgatggttg tagccgacat ggttgttcat gtttgaacgg
    30781 gcgttatgca ccaggaacgc atcgcgctga ttgagtaggg cgatgtcatc tttcgacaag
    30841 tacagcccat gagcgaccag cgttttgctg tcgatgagat cgaattgcgc cagtcgtgcc
    30901 agcaggtctt tgccgtacca gtggtgactg taggaaacgt cgtaaaggtc ttccgcagcg
    30961 tgaatatgca aaccacgacc tgtggctttc acggcttcac gcagcatctc cagaccggca
    31021 tctggcacgg taaacggcgc gtgagcaccg atatgtgctt ccaccagata cggctcggtt
    31081 gctgctttct tcgcctcatc aatctgacgg gcaaagcgga tgttttcttc tacaccttcc
    31141 tgcaactctt tgatgccgtt gttacggtca gtagtttcaa aacaggtcat tgcgcgcagg
    31201 ccaactttta aaaatgcgtc gcgcaatgtg gagagcgacc cgccgatata cgctggagag
    31261 gcatggtgat cgataaccga tgtacatccg ctcttaatcg cttccaggga acaaatcagc
    31321 ccgctgtaat agagcgactc ttcatcaagg gcgcgatcga gccgccacca gagatttttc
    31381 agcgttgaga tgaaatccgg gcagggggcg atgtttgcca taattccgcg ggaaagcccc
    31441 gagtaaaaat ggttgtgcga gcagacaatt cccggcatca caatccgacc atgcatctct
    31501 ttgtagctgg catcggggta gcgttgcgtc agggcatcgc cgatagcgac aatcacatca
    31561 ttttcgatag cgatatcaac ggcttcctgc actttcgccg ggtgtaactg cactgcggtg
    31621 acattcttca gaatcaacat gattacacct ccacgcggcc cagcagataa tgatgatgct
    31681 ggtggacatg gctgatgatg cggcacatat cgttcagctc cggtggtacg ttgttgaact
    31741 gaccttcgct gtcgatgttt aacacccagc tttggttatt cagacgtact cgtacccggc
    31801 aatcttccac aaggaagcct gggttgctgc tgttatcaaa gtcttgcgac aggctgaaga
    31861 cggtgatttt gtctttgtat ggtttaccgt tccacggaca gaactgagcg cagttgccgc
    31921 attcgttaca gtaagcgtcg aggtgcagcg tctggaaacg gttctggaag cctgggaccg
    31981 caatggagac gttggcgcgg ttcgggcaga catccacgca cttgctgcaa acgtagttac
    32041 attcaaggca gcgagcagct tcctgcgcga caaacgcatc acgatcgtca ctgttcacca
    32101 gcgtgataga gatatcgcct ttacgttgat agatttccgc cggattgacg ttgttccagt
    32161 atttatcgtt ctggtgggaa cggatatttt cccgactaag gatggcatcg gtcgcccgac
    32221 gcgcggttcc gacagcagcg acaatggagg atggtccgcg ctgtacgtcg ccgatcataa
    32281 agacgtcagt cagacgagtt tcgccattat ggtcgacgtc tggccagccg tttttgtcca
    32341 gcggcacgcc catcgcattc agggcttcag tatcctgctg ttcaccaatg gcggtgatca
    32401 ggctgtctac atgcagtgtt actgtttcgt tggtttcaac cggacgacga cgacctttct
    32461 catccggttc gccaagcgac ataacgcgca aggttaaggt gccatcagca tcgaaacgtt
    32521 ccggattatt caggaaacgg aactccacgc cgtcgtgcaa cgcttcttca tactcttcgc
    32581 gccatgcggg catctcttgc agtgaacgac ggtaaacgat cgttgctttt tttacgcctg
    32641 gaacgcgtaa cgccgcacga gcgcagtcca ttgcggtgtt acccgccccg acaacgacca
    32701 catgtttgcc cagcttgagc gctgtaccct tgttgtattc acgcaggaag gggagtgatt
    32761 tccagacatt ttggttgtcg cccgccagtt tcacaccgct atttttatca gtgccggtgg
    32821 caatcagaac atagtggaag ccctgatttt ttaactgttc aacggtcaaa tcgggtgagc
    32881 agccatactc aaatttcacg ccgtgagcgg caacaaaatc gatatcgtgc tgaattaact
    32941 ctgcaggaat acggaactga gggatgatat ttttcaccac gccgcccgca ttggcttcgc
    33001 gttcaaacag cgtaaccgga tggcccgctc tggcaaggaa gtaacctgca gccagacccg
    33061 ccggacctgc accaatcacg gcaaccggat ggcgtgaacc agaaccggct ggtttgtgcc
    33121 agcgttgctt atattcatcc caaccttttt ccagcgcgac ttttttcagt tcgcggatat
    33181 tcagtgcact gtcgtaatcc aggcgggtac agttgtattg gcactggtga tcgcaaatat
    33241 gaccggtaat ggcgggcagg gcgttgcgtt ggtagataag ttccagcgcg tcggcatagc
    33301 ggtgttcgcc aagcagacgg atgtattccg gaatatcttg cttgatcgcg caggcagtaa
    33361 cacagggggc aacgtagcag tcggtcagcg gcaggtcttc tgccacttca atacgctctt
    33421 ctggcttcca gtgtttctgg gtgtattcca tggttagcgc gtctgctgcc agtctgttca
    33481 gtcgttcgac gtcaacatgg tcaagtcccc aggcgtcgga gccttccagc tcgcgcatgc
    33541 aggcacttaa acgcagatag ccaccaggtt tcagcaggtc ggttgccata gtaatagggc
    33601 gaatacctgt atcaaaaata tcgcggatag tcagctgact ggcaccaccg gaataagaaa
    33661 tgggcagttt gccgtcaaag gcgcgagaga gaactgctgc aacattgatg gagagcggga
    33721 acagcgcacg gcctgacata tacatctctt caccaggcag tgcgccttta ttgttgatgg
    33781 tgccgagagt gttagtcagt tttacgccaa agccgagtga tttttctttt gccagtgcca
    33841 tcaggcgttc cagcatttcc agtgcttgcg tcagcttgag gtcgtgatca aatgactctt
    33901 cttttaagcc tatgtaaccg aaaccgcaga catcgaggat ctcacgaaca cgcgcgtacc
    33961 ccagtaaggt cgggttaagt ttcacaaagg tgttgagccc tttttcttcc agcatgtagc
    34021 ggcaaatggc ttcgatttca tgcggaggac agccgtgcat ggtggagagg gtgacgccat
    34081 gcaccatact ggtggggatg cgagcgggta aggcttgcaa gctttcgcgt ttttcctgca
    34141 atccgtggcg ggctaaaaat gcgtcatcct ggagtaattt attcagcgta tcgcgatatt
    34201 gcgcgaattt cggatggtca gatgcgtcca tcatattgtc gatgaactgt tgcattggcg
    34261 gttgcttaat accttcgagg ttgtaaccga cgctcatatt aaagatgaac gatttaccgg
    34321 aatcagaagg ttggaacatc gcttcgagaa ggtgcagggc aaaccaggct ttgaggtatt
    34381 catcccaggc tttaagcagg gtaaattcgg tagaccattc ggtgttaaag cactcgtctt
    34441 cggcatcaat acagggcttt tccagctcca ggcggtcaag aatttggacg gtttttagtt
    34501 cgatgaaacg tccgccagtc agccaggaag tgacaatatt ttgcgcgagc tgcgtgtgcg
    34561 ggccagcggc agggccgacg ggagtggcac aggtttcacc gaagacgcta acagttttac
    34621 cttttacagg tgagtaaaac tgttgctcgg gaataccaaa gattgagcgt tgttgttggt
    34681 attcatcaaa tatgcgcgtc aaaagttcct caaacggaat gggacgcata atatccccca
    34741 taaccctctc cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg
    34801 tttaggggga tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa
    34861 aatgtgaggc ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga
    34921 gaattaaaat cactcctgaa aattattatt gccagcccat atatcacttt ttctcttttc
    34981 tcaaaatcag tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa
    35041 cgcaatcgtt taactacgtt ttttaaattt tagtatacct tttttgcgct gtaataaagt
    35101 cgtccggagt atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag
    35161 ctcccataag gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca
    35221 ggcatggttt tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg
    35281 cgccatcatt tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt
    35341 caccgtgggt gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg
    35401 ttagtaaacc ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcgcat
    35461 aacgttcgtg cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact
    35521 gcaacgcatt tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt
    35581 tccattgtcc cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca
    35641 tagtggttcc attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg
    35701 attaagttgg ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga
    35761 cccatcggca ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt
    35821 tggtgctggg gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg
    35881 caggcgtgtg ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt
    35941 ggttttctgt cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt
    36001 ttgttttcac agctggaaag cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat
    36061 tgatgcactg gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg
    36121 tgcaatgccg ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg
    36181 cgtgattgct gtgatgggag gacatatttt gggcgcgaaa gtgagcacag aaaatgtcca
    36241 tcgctggtcg cagtttgctg atattaccgg gttaacacct gatgcaacct tgcaactgag
    36301 cgatctcgtt gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg
    36361 tcggcgagtc tggttcatta accgtttttc tcaatgtgag aatgcgattg cgcaaagcga
    36421 gctccttcaa ccgctgcaac aacacgacgt agaggcaatc tggctgggcg atatacaaga
    36481 acatcctgca atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa
    36541 aggatatcca ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa
    36601 aattgtccgt ttgcgatggc gcaaattgtt gatagccgag gctcgactcc ccgccattct
    36661 gcacaaatgt tagtgcgcgc cgatggttct atcgtcggta caattggtgg cggaatggtt
    36721 gagcggaagg tgattgaaga gtcacttcag gcattgcagg agcgtaagcc gcgattattc
    36781 catggacgta tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg
    36841 tcagtattta tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat
    36901 gtcaaccggg cgatagccca gagtgcggcg ctattaggat ttgatattgc cgttgccgat
    36961 atttatcgcg aaagcctcaa tcctgaacta ttcccaccat caaccacgct tctccatgct
    37021 gagtcgtttg gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt
    37081 gccacgaata atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg
    37141 ttggggttgc tggcaagtcg tcgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa
    37201 ggcgtggctg aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg
    37261 gaaacgccgc aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac
    37321 gcgccgggtg ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt
    37381 cgcggtgcgg gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa
    37441 gtgatcatgc tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag
    37501 gccgtgttcg atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct
    37561 gcggaagcga tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt
    37621 tcattgcttg atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat
    37681 ttggggacgc gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttattgca
    37741 gggaaggatt gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt
    37801 tactctggtt gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc
    37861 cgacgggtga tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat
    37921 ctggtgaaag agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg
    37981 acggggatgg tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt
    38041 ggtgatatcg atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg
    38101 gcgattggcg gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg
    38161 acaaaagaag tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc
    38221 cttaaaatta ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc
    38281 tcaatacggt aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt
    38341 ccactttagg cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat
    38401 cacttaatga caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt
    38461 tttctaccgc agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc
    38521 tggcccagtc tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa
    38581 cagggatacc accaccgcca acggtgatca ccacctggcc tgcatctacc agagctttaa
    38641 ccgtttcttt ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc
    38701 gaccagcatc ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg
    38761 taaagaacga accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt
    38821 cgacctgagt aaccagcgtt gctacaggtt tattaatgcc ccgagaaagc agttcttctc
    38881 ttaacgcgtt ttgtagatcg taaccaatat aaccctggct taaggcaaca cagacagaca
    38941 tcggcagcat tggcgagtgt gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca
    39001 tgcccacctg tggcccgtta ccgtgagtga cgatgacctc atgtccctgg gcaattaaat
    39061 caacaattgc ctgagaagta atttttaccg ctttcatttg cccggccaga tcatcgccca
    39121 gcgcattccc gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct
    39181 tgattgtgta tagatcccta gcagtggtta gagcacggga gggacaaacg gtttgcgtcg
    39241 caggaaacga cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc
    39301 acgggataag gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc
    39361 ggcgttgtcg tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac
    39421 cacgtcgcca tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct
    39481 ggcgggcatt gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac
    39541 gccactggaa aacagtaact gcatgcggtt ttccacgccg ggtaagccat ttgggcagcg
    39601 actgaaatcg cctttagaaa tttgcaggcg ttgagccatt gagaacgtgc agtgatcggt
    39661 cgccaccacg tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtat
    39721 gttacgcagc ggtgggctaa gaatgaactt catgccatct tctgtatcgt aactgcgttc
    39781 gtccaacagg agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc
    39841 aagacgcaga taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt
    39901 accggcaatt tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg
    39961 gcgactcaag gcgtgatagc gcggcgcggt taaccctgcg gcgataaact ccgcccgctt
    40021 gctggcgata gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcag
    40081 acggcgtaat gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag
    40141 tttaaaactg ctcagccctt cctcgaccat catagggatt tcgtcgagga tggcgtgatt
    40201 aatgtgctgg atcacaccat gaaagctgta gtcgatgacc gccttatggg cggcatagcc
    40261 acgataaacc tccagttgat ggcgtaaccg acacccgttt gggccaaatc ccatatggtc
    40321 aataatggtt gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca
    40381 actgcgcgcg atgccgacat caatattgaa atgcgtatgg acatccacgc cgccagggaa
    40441 aacgtaacag ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc
    40501 caactggcga acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt
    40561 aacgacagtg ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa
    40621 ccggagggga ataagtgaag agtaattatt cggttgccag ccagcttaac gggattgcag
    40681 cgtacatcgc agcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg
    40741 cttcaggttc tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag
    40801 atacgccgtt agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt
    40861 cgtaggcatt caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga
    40921 agtagcattc ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag
    40981 aaacaaccgc gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt
    41041 cccaggtttc gccccaggtc agacgacggt cgattgaaac tgcgcagctg tctgctacag
    41101 cgcaacggct tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga
    41161 attcgtcata acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt
    41221 tgaaaatggc gttatcaccg cgttctggtg cagaaccgtg gcagctaaca ccctgaacat
    41281 caatacgaat ttccatacga ccgcgctgac cacggtatac ctggcagtcg gttggttcgg
    41341 tactgaccac aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac
    41401 cgtcgcagtc ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac
    41461 cgaggtcttt aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg
    41521 aagtaccgcg accaccgatt agctcatcag tttccatgcc ttcgtacgga tcgaagtccc
    41581 agttcttgat gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg
    41641 ggccgtggcc gatataaccg agaacgttgc ccatcgggtc gatttcaact ttatcgaagc
    41701 cgactttttc catctcttct ttaatacgat gtactacgcg tttttcgtcg cagctttcac
    41761 tgggaatagc aaccatgtcg cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt
    41821 tttcaagaat cagtttgaat ggaatattct tagccattat ttttctccag attttttact
    41881 ccccgttttg cagggagtag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt
    41941 cccagacaac ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca
    42001 ctacggcatc tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat
    42061 gaaccgctgc gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt
    42121 cgttgccgta cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg
    42181 agatgaattg ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac
    42241 aggccaggcc cgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga
    42301 cgccggagcg ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt
    42361 gcgggctgta gacgtcgacc agataaccca gcacaccacc ggccattgca ccgacaccgg
    42421 cttgcagcag aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat
    42481 ctgccagggt tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg
    42541 ccgtgtcctg taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg
    42601 tatcgtcata gttcatatct gtgacgatgc actcggcacc gaggttcaga atggcgtcaa
    42661 cgcgttcctg agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgtg
    42721 ctgcccacgc cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt
    42781 cgccgatggc atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt
    42841 cacacaataa ttgagcgatg gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga
    42901 atcgttttga ttcgtctttg acgagaattt tcttcacacc aaaaaggttt gcgagatcgt
    42961 ccagcgcaca aagcggtgtt gggcgataac cagctatttt ctggtggaac tggcgcgcca
    43021 gtttggcctg gctttgcgaa aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg
    43081 cgatatcaat cttcaatgag aaaacggaca tatcctatcc ttttggcctc tggaaataga
    43141 gggccgacca gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct tctttcagca
    43201 gttgctcgag cagtgcacct ggtttggcgt atttacggga caggatcatc gcggcgatga
    43261 tataaggctt ccagctggct tctttgtagg tagcgatacg gtatttttcg aatacgcctt
    43321 cagtcacttc accttctttg caggatacgc cgctgatatc agctggcagg cagtgcatgt
    43381 acagggcttc gccatcacgg gtcagttcca tcatctcttc agtacaatgc cagtctttgt
    43441 gttgcgcgtt ctgtgccaga cactgttttt ccagtgcttt taagccttcg tggtcgttcg
    43501 cacgcagcaa ttcagtacgc tcttccatca ctttgtaagg tgcccatgac ttcggataaa
    43561 cgatgtctgc gtctttgaag gcttcttcca tgctggtgac ctgacggaag ctaccaccgg
    43621 aggctttagc attgtttttc gccacttcaa ccacatccgg gatcaggtcg tagccttccg
    43681 gatgggccag ggtgacatcc ataccgaagc gagtcatcag accgatgatg ccttgtggta
    43741 cagagagcgg tttgccatag cttggagagt aggcccaggt catggcgatt tttttacctt
    43801 tcaggttttc cagtgaacca aagtgttcac gtaaccacgc gaggtcagcc attgactgag
    43861 tcgggtggtc aatatcgcat tgcaggttca ctaaagccgg acgctgtggc agtacaccct
    43921 gcttgtaacc gtcatcaagt gcagcgccaa cttcacgcat ataggcgttg cctgcgccca
    43981 gatacatatc gtcgcgaata ccaatagcgt cggcgcagaa ggagatcata ttggcggttt
    44041 cacgcacggt ttcgccgtga gcgatttgtg atttgccttc gtcgagatct tgttgtgcaa
    44101 ggccgagcag gttaagcgcg gaagcataag agaagcgggt acgggtggag ttgtcgcgga
    44161 atacggaaat acctaatcca ctattaaaga ctttggttga gatgttttca gcacgcagtg
    44221 cttttaatgc tgcggcaacg tccagtactt gtttcagttc atctggcgtc tgttcccacg
    44281 ttaacaaaaa atctttctcg tgaaggtgag aggtcagcga attgatatcc ttaatcagct
    44341 cattaacagt tttcatcata aagtcccggt agtggaatga caggcaaagt ggtttatttc
    44401 actgtgactt gccaaagtga ttggtgtttt cacagctata cctgggacta acaataagtg
    44461 tgccagaatt atccgcgcgg ctgcaaaaga ctaatttgac ggataatgtg tggggttgat
    44521 cacaaattga tgccagcttc tggttgtttg taaaatacga aaacgtttgg ctttttttat
    44581 cgcgttattt atttgctgct aagtatcaga aataagataa aggggcaggc tatctctctt
    44641 cagtttgata aactcaccgc cgtaattctc atattgatat agaaatgtga tatctcttcc
    44701 ataatttcat tagaaaatga taaaaatctt atgtttttat caaagccaat ttcgcctgag
    44761 ttaatatcat catgatctgt gaaccatcaa cgtcttcaat gatcgacttt taagtaagtc
    44821 tggaaggtaa agctatggag cttgctacta cgcagtcagt attgatgcaa attcaaccga
    44881 caattcagcg ttttgccaga atgcttgcca gcgttttgca gcttgaggtt gagatcgttg
    44941 atgaaaactt gtgtcgcgtc gccggaacgg gcgcgtatgg gaagtttctt ggtcgccagt
    45001 tgagcggcaa ctcacgcctg ctccgccacg tcctggaaac gaaaactgaa aaagttgtga
    45061 cacagtctcg cttcgatccc ctttgcgaag gttgcgatag taaagaaaat tgccgcgaaa
    45121 aagcatttct gggtacgcct gtcattttac aggatcgttg tgttggggtg ataagtttga
    45181 ttgccgttac ccacgagcaa caagagcata tcagtgataa tttacgcgaa ttttctgatt
    45241 atgttcgcca tatatccacc atttttgttt cgaaacttct ggaggatcag gggccaggag
    45301 ataacatcag taaaatattc gcgaccatga tcgataatat ggatcagggc gtattagttg
    45361 ttgatgatga aagtcgggtt cagtttgtta atcagactgc cttaaaaaca cttggtgttg
    45421 tacaaaataa tattattggg aaacctatcc gtttcagacc attaacattt gagagtaatt
    45481 ttactcatgg acatatgcag catattgttt cgtgggacga taaaagtgaa ttaatcattg
    45541 gtcaattgca taacattcag ggccgacaat tatttttaat ggcatttcac caatcgcata
    45601 ccagtttttc tgtagcaaat gcacctgatg aaccacatat tgaacaattg gttggcgagt
    45661 gccgtgttat gcggcaatta aaacgactca ttagccgtat tgcacccagc ccatccagcg
    45721 ttatggtggt tggtgaaagc ggcacgggta aagaagttgt cgcccgtgca atccataagt
    45781 tgagcggaag acggaataaa ccctttattg ctatcaactg tgccgcgatt ccggagcagc
    45841 ttctggagag cgaactgttc ggttatgtta aaggcgcatt tactggcgct tctgccaacg
    45901 gtaaaacagg gttgattcag gcggcgaata cgggcacgct gtttctcgat gaaataggtg
    45961 atatgccatt aatgttgcag gctaaattac tgcgcgctat tgaggcacgt gaaattctgc
    46021 cgattggtgc cagtagccca atacaagtcg acattcgcat catttctgca actaaccaga
    46081 atttggccca gttcattgcc gaaggtaaat tccgcgaaga tctcttctac cgacttaatg
    46141 ttatcccgat aactctgcca ccgctgcgtg aacgtcagga agatattgaa ctactggtgc
    46201 attacttttt acatctgcat acccgtcgtc tgggatcggt ttatcctggc attgctcccg
    46261 atgtcgtcga aatattgcgt aagcatcgtt ggcccggaaa cctgcgcgag ttaagcaatt
    46321 tgatggaata tctggttaac gttgttcctt caggtgaagt tatcgacagc acgctattgc
    46381 cgccaaatct gctgaataat ggcacaacgg agcaaagtga tgtaacagag gtcactgagg
    46441 cacacctgtc actcgatgat gcgggcggca cggcgctgga ggagatggaa aagcaaatga
    46501 tccgcgaggc gctttcacgt cataacagca agaagcaagt tgctgatgaa ctgggcatcg
    46561 gcattgctac gctctatcgc aagattaaga aatatgagtt gttaaacaca taacctcagt
    46621 gaatccggcc tgaattcagg ccggatatcc ttttacttcg ttttctcgca atccagaacc
    46681 gtatttacaa tcatctgata ccccgtgcag cgacaaagat ttcccgccag tccgcgacga
    46741 atttccgtaa tggttaatgg cttctcgcgt ggtttcgcca gcattgccgt ggtagccata
    46801 atcaggccag gcgtacaaaa cccgcactgc accgcgccgg atttcgcata agcctgctga
    46861 acatgagaaa gttttccgcc tttcgcttca ccttcaagcg tgcggatctc ttttccttca
    46921 gcccaggcgg caaggtataa gcaactgtct attgctgtgc cgtcgaccag caccgtacag
    46981 gcaccacatt cacccacgca gcacccttgt ttgacactta gcagtccttg ttcgcggagt
    47041 aattccgaga gcggcgtacc tggtgcggca tgaagctgaa aaggcatccc gttaatggtg
    47101 cattcgatgg taattgtttc gctgtgattc attgcaattt tccccccgcc gcggcgacgg
    47161 cttcgctaat cacttttttg gtcatcgtct ggatgagatg cagacgaaac tctttactgg
    47221 cccgccatga agaacgcggg gcgacatctt gcaggacaga ttcgctgata gcttccagcg
    47281 tttgcaggtt taatggcgca ttttgtgcag tctgttcggc atgttggcag cgaatcggcg
    47341 ttggcgcggc aacaccaaat gccaggcgta attcgctgaa attgccgtta tccagtcggc
    47401 aatgtgcggc acagccaatc gttgaaatat ccattgcgtc gcgcatggca tatttaaaat
    47461 gcgcgctgcc cacgtgttct ttcggctggg gcggaaaatg aaaggcgaca aggatttcgt
    47521 catgctcaag agacactttg cctggcccag tgtgaaagcc attaatcggg acgaaacgaa
    47581 caccgcgtgg ggagtggatc tccagctttg cgtcataaat tagcgttggc gtggcagaat
    47641 ctgcgctggt ggcaccgttg caaatatttc caccgtaggt agcgacgtta cggatctgcg
    47701 gtccggcaat ggatgaggcc gcagcacata acgccgggag atgacgttga gttacaggat
    47761 cttcaatgag ctgggtaaat gtcgttgcag agccgatacg tagtgagcca tcttccgcca
    47821 gcgtaattcc ccgcagctcc gccagattat gaatatcaac aatatggcga taacggtcat
    47881 tgtgatggtg gagctgaatc agtacgtcag tgccaccggc gagcagtttg gcctgcgggt
    47941 tgtcaaccag caggttgatg gcatcggcaa gggttgctgc gcgatggtaa gaagcaaaat
    48001 caaacatgat gttatcctca aatcaatcct gccagatgga actcttcata taaccgtttt
    48061 ggcgtcagcg gcagtgtatt gattgcaaca ccggtagcca tcttcaccgc gttacgaata
    48121 gctgcggcaa caggaattat tggtggctca cccagtgact tatgtccgta tgcggattgc
    48181 ggctcattga tttcgacgaa cgcgctttcc agttgtggca gatccggcat ggtcggcatt
    48241 ttgtaatcca gcagattggg gttacggacc acgccgcttt tcgcatcgat gatcatctct
    48301 tcaaatagcg cccagccaat gcccattccc attccgccgt gtacctgacc ttctgccagc
    48361 agcggattta gaatatgccc cgaatcatga acgttgagga tgcggttgat ggtgactttg
    48421 cacagtgcaa tatcgaccgt cagatcaaca aaggtacagc cgaacgccgg tgggttagtg
    48481 gtggttttga tggagctttc agcagagagc tgcccgccgc gttcagggtg gtagaaagcg
    48541 tccatcgcca aatcttttaa cgacattaac ggctcttccg gtcgttcaac cagcacgata
    48601 tggcctttta tcagggtcag attcatcgct gactgatgta gcatgactgc ggcgtgagcg
    48661 atgattttct cttttaataa cagtgccgca ctgcgcagcg caggcgcggc aacatagctc
    48721 tggcgcgagg caaatgcgcc gggatcgaac ggcgtaacgt cggtatcttg tgttgaaata
    48781 acgcgaacgt cgctgaccgg aacccccacg gtttctgcca ccatttgcga gaagacggtg
    48841 tcggcaccct gaccgatttc cgtcgcgccg ctttgcacgt tgatggttcc atcctgattc
    48901 atcagaaggc gcgcgcctgc tatttctacg ccgacaggcc aggtattaga ggtgtagcta
    48961 aaacaggcga cgccaacgcc gcggcgtaaa ttgccttgct ggttctggca ttctgcacgg
    49021 cgtttttccc attcaaagat tttccggcct ttttcaagac actccggcaa ccctgcgctg
    49081 taaatacgtt tgcccgtgag cggattagca tctccttcgc gggcggcgtt gcgtaaacga
    49141 atttcaacag gatcaatacc taacgctgtc gcggcgtcat caagcataga ctcaacggca
    49201 aatacgactt gtggcgcgcc ataaccacgc atcgcaccag ccgaggggag gttggtatag
    49261 caggtctttg aactgtaagc gtaggcacaa cgaggataaa ggtaagcgac tttattcccc
    49321 cccgcagaag cgatggagtg cccgtgagat gcataagcgc cggtgttaga cagaacatcc
    49381 agactataac ctttcaatgt tccgtcgcgg ttcacgccca tttgcccgtc aatagtaaaa
    49441 gcgtggcggg tacgggttgc gaggaaacac tcttcacggc taagggaaac tttcaccgga
    49501 atgccgccaa gcttgctggt caggaatgcc gccattggct cttccagtac atcctgttta
    49561 ttaccaaaac cgccaccgac aaatggtttg atgactcgta cgcatgacca gggaatatcc
    49621 agcgcctgac caaccacgcg gcgaacaatg tgcgggatct gggtgctgga aacgatggta
    49681 attcgcgagt catcctccat ccacgccagc gatgttacgc tttccatgtg acaatgttga
    49741 ataacggggg tctgatagtg cccctgtacc tggtagtcgg cggcatcgat tgtttgttgg
    49801 acattgcccg tcgacatcgt gctttgtttc agtaaattgc cgccgttatg gattggtgca
    49861 gcgtcctctg ccagcgccgc ttctggcgtg gtgataacgg gtaattcttg ccactcaatg
    49921 ctgaccaatt gcgccgcttt ttctgccgtg agttcatcgc gggccacgac gatggcaacg
    49981 gcgtcaccat gatgacgaac atggcgagtt agcagtgcgc gatcggcggt atcgcgcttg
    50041 ttttcgtcaa gtgtccatgc atgtccagct gtagcgaatg ggatatcagg cacatcttcc
    50101 caggtaaaaa tcgccagtac gcccggtaaa cttctggctt gttcatcatt aatacttacg
    50161 gcataaccat gtgcgatagg gctacgtaca tatttcgcat aacacatgcc cgccataaca
    50221 taatcgtcag tatatcgtgc ccgcccggtg accttagcaa tggcatcgac gcgcatgcat
    50281 gattcacccg tagcggttgc ttcccgcgct tccatagaaa tcccctcgat ttgaacattt
    50341 ggttcttatg gcaaagattt agtctaagca ggcaagattt acgccagaaa cgacgataag
    50401 cgttagggga ggaaggaagt gtgatgacgt taaaaaaaca cagatatgat acgaaacagg
    50461 actctgaata gggacagtga tacgtatcaa tatgagtggc gataaaatca atgtgataaa
    50521 tcaggattat tacgcatcag gatgggaaat agggtaaaaa gtcaggaact tcgatacctc
    50581 tacagcgaat attttgctga tgatacttcc ctttgccaaa tgaaattaac ctctttctct
    50641 attaccacgt ttttccagaa gcaagagatt gggtatcaag aggctggctg ctatgatatg
    50701 gcgtcttgtt ttttaagcga ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc
    50761 tgggtatcgt gatgttgtta tcggttggac tgcttttggc gggctgttcg ggtagcaaat
    50821 catccgatac aggaacgtat tccggctccg tttacaccgt gaaacggggg gatacgctat
    50881 atcgtatttc gcgcaccacg ggaaccagcg taaaagaact ggcgcgactg aacggcattt
    50941 ccccccctta caccattgaa gttggtcaga aactaaaact gggtggggcg aaaagtagca
    51001 gtattacacg taaatcaacc gccaaatcaa cgaccaaaac cgcatcggtt acaccgtcat
    51061 cagcggtacc gaaatcatcc tggccgccag tagggcaacg ttgttggtta tggccaacga
    51121 cagggaaagt tatcatgccg tattcgacag cagatggcgg caataaaggg attgatatct
    51181 cagctccacg gggtacacct atttacgccg cgggtgcagg aaaggtggtg tatgtgggca
    51241 accagctgcg tggctacggt aatctcatca tgattaaaca cagtgaagat tacattacgg
    51301 cttacgccca taatgacacg atgctggtaa ataatgggca aagcgtgaag gctgggcaaa
    51361 aaatcgccac tatggggagc acggatgcgg catctgttcg cctgcatttc cagattcgtt
    51421 accgtgcaac ggcaattgat ccgctacgtt acttgccgcc gcagggcagc aagccaaaat
    51481 gctgatggcg aattaatcag cagtcagcag cgcggcactt gctaaggaga gcgtaaggtt
    51541 tataatgcct tacgcatctc gaagcgggcg tagttcaatg gtagaacgag agcttcccaa
    51601 gctctatacg agggttcgat tcccttcgcc cgctccaatt tatcttcacc tcatcaatct
    51661 gccatttttg ctgtatctga ttttctatat tatcttaatt caatcgcttc agacttttcg
    51721 caatcgccgt aatatttccc cattttgttg taaataaaaa gcgtttacgc aatgcaatcc
    51781 ttttgtaaca acccaggtaa aacaccttat tttcaagttg ttaaataagt ggttatctac
    51841 gcttttaaca aacgattgat ggaatatttt atgaaaggga aatctgcatt aactcttctg
    51901 ctcgctggta tattttcgtg cggcacatgc caggcgacag gggcagaagt gacgagtgaa
    51961 tctgtattta acattcttaa ttccacgggg gcagcaacag ataaaagtta tttatcgtta
    52021 aatccagata agtaccctaa ttatcgtctt ctgattcact cagccaaatt aaaaaatgaa
    52081 ataaagagcc actataccaa agacgaaatt caagggttgt taacattaac agaaaacaca
    52141 cgaaagttaa cgttgacgga gaaaccctgg ggaacattta ttcttgcttc tactttcgaa
    52201 gatgacaaaa cagcggcaga aacccactat gatgctgtct ggctgaggga tagtttatgg
    52261 ggctatatgg cattagtatc agatcaggga aatagtgtag ccgcaaaaaa ggttctgctc
    52321 accctgtggg attatatgtc tacgcccgac cagattaagc ggatgcagga cgtcattagc
    52381 aaccccaaac gactggatgg agttcccggg caaatgaatg ctgtgcatat ccgatttgac
    52441 agcaactccc ccgtgatggc agatgtgcag gaagaaggca agccgcagct gtggaaccat
    52501 aaacaaaatg atgcgttagg tctttatctt gatctcctta tacaagcaat cgacactggt
    52561 acaattaacg ctgaagattg gcaaaaaggt gaccgattaa aatcggttgc gctgctgatt
    52621 gcttatctgg ataaagccaa tttctatgtt atggaagatt ccggagcatg ggaagaagat
    52681 gcccgattaa acacatcttc cgttgcactg gtgacctctg gactggaacg tttgtcgaac
    52741 ttactctcga agaaagactc tgtttttgtc tctgatttac tacgcgaagc taaagccaat
    52801 gaactggatg aacctctttc aacaacgagg ttgaaccacc tcatagataa aggttatgaa
    52861 agaataacgt tacagcttga tttaggggga gaatccccag gttatctgga aaaagataaa
    52921 cattacagag aagccgatgc ggctttactt aatgttattt atcctgcaaa tcttgcaaag
    52981 ataaacacca gaagaaaaga gcaggttcta aaaatagtta aaaaactagc aggtccgtat
    53041 ggtataaaaa gatacgagaa ggataattat cagtcggcaa atttttggtt caatgatatt
    53101 aaaaccgata ccgatcaaaa ttcacatgtg aaaagagaaa agagcttcat tccatcaaca
    53161 gaagcagaat ggttttttga ctcatggtat gcaaaatcag cagcaattgt ttataaagaa
    53221 tcaagaaaag aagagtattt gaatgactca gtgcaattta tgaatagatc attggcccaa
    53281 ataacaggcg agaatatgat tggtgcaaat ggcaggagtg tacctgaaat ggcacttccc
    53341 gaaagctata actatatcca caagtcggga actttgcatg aagcgccaaa ccctatcatt
    53401 ccgctaaatt ggtcgaaagc ttcaatgact ctcatgttga aagagatgtc cagccttatc
    53461 aatgatgagg gaaataaata gttttcataa tgaggcggtc ctggattaaa aggaccgctc
    53521 aaagagaaat cgaaagggga ggtaaaagtg atcaataaat tactgttagc ttacctgata
    53581 ggtctggttg tgacatcgac ttttatattt atcttctcgg aagaaaaagt aacatatcgg
    53641 ttgtttgcta caataattac aggtttaacc tggccgctta gtctcattcc ttccattata
    53701 agtctcatga ttcgtaaatc cgactaacaa gaaaattata tgatcgatta agaaaaatag
//
LOCUS       DAPHJE010000012        53250 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPHJE010000012 DAPHJE010000000
VERSION     DAPHJE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.012"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..148
                     /locus_tag="RH222_000773"
     CDS             <1..148
                     /locus_tag="RH222_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV3795833.1"
                     /translation="ALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGCG
                     PTAQ"
     gene            392..1780
                     /gene="frlA"
                     /locus_tag="RH222_000774"
     CDS             392..1780
                     /gene="frlA"
                     /locus_tag="RH222_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDV3795834.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1801..2823
                     /gene="frlB"
                     /locus_tag="RH222_000775"
     CDS             1801..2823
                     /gene="frlB"
                     /locus_tag="RH222_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDV3795835.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNEEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2873..3703
                     /gene="frlC"
                     /locus_tag="RH222_000776"
     CDS             2873..3703
                     /gene="frlC"
                     /locus_tag="RH222_000776"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDV3795836.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3700..4485
                     /gene="frlD"
                     /locus_tag="RH222_000777"
     CDS             3700..4485
                     /gene="frlD"
                     /locus_tag="RH222_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDV3795837.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4585..5316
                     /gene="phoB"
                     /locus_tag="RH222_000778"
     CDS             4585..5316
                     /gene="phoB"
                     /locus_tag="RH222_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDV3795838.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5468..6553)
                     /locus_tag="RH222_000779"
     CDS             complement(5468..6553)
                     /locus_tag="RH222_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3795839.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDAQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6565..7869)
                     /locus_tag="RH222_000780"
     CDS             complement(6565..7869)
                     /locus_tag="RH222_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV3795840.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7881..8234)
                     /locus_tag="RH222_000781"
     CDS             complement(7881..8234)
                     /locus_tag="RH222_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV3795841.1"
                     /translation="MKKIGVAGLQREQIKKNIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8245..9123)
                     /locus_tag="RH222_000782"
     CDS             complement(8245..9123)
                     /locus_tag="RH222_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV3795842.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9120..10346)
                     /locus_tag="RH222_000783"
     CDS             complement(9120..10346)
                     /locus_tag="RH222_000783"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV3795843.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10346..11509)
                     /locus_tag="RH222_000784"
     CDS             complement(10346..11509)
                     /locus_tag="RH222_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV3795844.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKIYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11593..11955)
                     /locus_tag="RH222_000785"
     CDS             complement(11593..11955)
                     /locus_tag="RH222_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV3795845.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11972..12877)
                     /locus_tag="RH222_000786"
     CDS             complement(11972..12877)
                     /locus_tag="RH222_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795846.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13146..14150)
                     /gene="trpS"
                     /locus_tag="RH222_000787"
     CDS             complement(13146..14150)
                     /gene="trpS"
                     /locus_tag="RH222_000787"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV3795847.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14143..14901)
                     /gene="gph"
                     /locus_tag="RH222_000788"
     CDS             complement(14143..14901)
                     /gene="gph"
                     /locus_tag="RH222_000788"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV3795848.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14894..15571)
                     /gene="rpe"
                     /locus_tag="RH222_000789"
     CDS             complement(14894..15571)
                     /gene="rpe"
                     /locus_tag="RH222_000789"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV3795849.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15589..16425)
                     /gene="dam"
                     /locus_tag="RH222_000790"
     CDS             complement(15589..16425)
                     /gene="dam"
                     /locus_tag="RH222_000790"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV3795850.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16532..17818)
                     /gene="damX"
                     /locus_tag="RH222_000791"
     CDS             complement(16532..17818)
                     /gene="damX"
                     /locus_tag="RH222_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV3795851.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVRTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17910..18998)
                     /gene="aroB"
                     /locus_tag="RH222_000792"
     CDS             complement(17910..18998)
                     /gene="aroB"
                     /locus_tag="RH222_000792"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV3795852.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19055..19576)
                     /gene="aroK"
                     /locus_tag="RH222_000793"
     CDS             complement(19055..19576)
                     /gene="aroK"
                     /locus_tag="RH222_000793"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV3795853.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19977..21215)
                     /gene="hofQ"
                     /locus_tag="RH222_000794"
     CDS             complement(19977..21215)
                     /gene="hofQ"
                     /locus_tag="RH222_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV3795854.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21127..21531)
                     /gene="hofP"
                     /locus_tag="RH222_000795"
     CDS             complement(21127..21531)
                     /gene="hofP"
                     /locus_tag="RH222_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV3795855.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21521..21961)
                     /gene="hofO"
                     /locus_tag="RH222_000796"
     CDS             complement(21521..21961)
                     /gene="hofO"
                     /locus_tag="RH222_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV3795856.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHQQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21945..22484)
                     /gene="hofN"
                     /locus_tag="RH222_000797"
     CDS             complement(21945..22484)
                     /gene="hofN"
                     /locus_tag="RH222_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV3795857.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22484..23263)
                     /gene="hofM"
                     /locus_tag="RH222_000798"
     CDS             complement(22484..23263)
                     /gene="hofM"
                     /locus_tag="RH222_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV3795858.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23383..25935
                     /gene="mrcA"
                     /locus_tag="RH222_000799"
     CDS             23383..25935
                     /gene="mrcA"
                     /locus_tag="RH222_000799"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV3795859.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26100..26660)
                     /gene="nudE"
                     /locus_tag="RH222_000800"
     CDS             complement(26100..26660)
                     /gene="nudE"
                     /locus_tag="RH222_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV3795860.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26980..29115
                     /gene="igaA"
                     /locus_tag="RH222_000801"
     CDS             26980..29115
                     /gene="igaA"
                     /locus_tag="RH222_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV3795861.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTNIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29180..29848
                     /gene="yrfG"
                     /locus_tag="RH222_000802"
     CDS             29180..29848
                     /gene="yrfG"
                     /locus_tag="RH222_000802"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV3795862.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29859..30260
                     /gene="hslR"
                     /locus_tag="RH222_000803"
     CDS             29859..30260
                     /gene="hslR"
                     /locus_tag="RH222_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV3795863.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30285..31163
                     /gene="hslO"
                     /locus_tag="RH222_000804"
     CDS             30285..31163
                     /gene="hslO"
                     /locus_tag="RH222_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV3795864.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31298..33022)
                     /locus_tag="RH222_000805"
     CDS             complement(31298..33022)
                     /locus_tag="RH222_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV3795865.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33401..35023
                     /gene="pckA"
                     /locus_tag="RH222_000806"
     CDS             33401..35023
                     /gene="pckA"
                     /locus_tag="RH222_000806"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV3795866.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35099..36451)
                     /gene="envZ"
                     /locus_tag="RH222_000807"
     CDS             complement(35099..36451)
                     /gene="envZ"
                     /locus_tag="RH222_000807"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV3795867.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36448..37167)
                     /gene="ompR"
                     /locus_tag="RH222_000808"
     CDS             complement(36448..37167)
                     /gene="ompR"
                     /locus_tag="RH222_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV3795868.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37395..37871
                     /gene="greB"
                     /locus_tag="RH222_000809"
     CDS             37395..37871
                     /gene="greB"
                     /locus_tag="RH222_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV3795869.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37968..40289
                     /locus_tag="RH222_000810"
     CDS             37968..40289
                     /locus_tag="RH222_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV3795870.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40727..40954
                     /gene="feoA"
                     /locus_tag="RH222_000811"
     CDS             40727..40954
                     /gene="feoA"
                     /locus_tag="RH222_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV3795871.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40971..43292
                     /gene="feoB"
                     /locus_tag="RH222_000812"
     CDS             40971..43292
                     /gene="feoB"
                     /locus_tag="RH222_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV3795872.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43292..43528
                     /gene="feoC"
                     /locus_tag="RH222_000813"
     CDS             43292..43528
                     /gene="feoC"
                     /locus_tag="RH222_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV3795873.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43731..44609
                     /gene="rpnA"
                     /locus_tag="RH222_000814"
     CDS             43731..44609
                     /gene="rpnA"
                     /locus_tag="RH222_000814"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDV3795874.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44638..45408)
                     /gene="bioH"
                     /locus_tag="RH222_000815"
     CDS             complement(44638..45408)
                     /gene="bioH"
                     /locus_tag="RH222_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV3795875.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45446..46129
                     /gene="gntX"
                     /locus_tag="RH222_000816"
     CDS             45446..46129
                     /gene="gntX"
                     /locus_tag="RH222_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV3795876.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46188..46763
                     /gene="nfuA"
                     /locus_tag="RH222_000817"
     CDS             46188..46763
                     /gene="nfuA"
                     /locus_tag="RH222_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV3795877.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47124..48440
                     /gene="gntT"
                     /locus_tag="RH222_000818"
     CDS             47124..48440
                     /gene="gntT"
                     /locus_tag="RH222_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV3795878.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48485..50569)
                     /gene="malQ"
                     /locus_tag="RH222_000819"
     CDS             complement(48485..50569)
                     /gene="malQ"
                     /locus_tag="RH222_000819"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV3795879.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50579..52972)
                     /gene="malP"
                     /locus_tag="RH222_000820"
     CDS             complement(50579..52972)
                     /gene="malP"
                     /locus_tag="RH222_000820"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV3795880.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        12604 a        14641 c        13488 g        12517 t
ORIGIN      
        1 tgcactggta ggtgcagtac tggctacgtt aactgcatgt actggtcata ttgaaaaccg
       61 tgataagaac tgctcttacg actacctgct gcacccggca atttctattt ctaaaatcat
      121 tggcggttgc ggtcctactg cacagtaagt ctctgacaga aatcggctaa caccggttta
      181 attgcccgac gccctctgga ttttccgagg gcgtatttgt ttctatgtcc ttcttgcccc
      241 cgcccgtaaa aatattttca ctttaaattc aatttgataa atacatccat tagccacagc
      301 gcgatcctgt gcgaaatttt gtgatcttcc tccacattac ataacatcat atgttgttat
      361 attcatcatg cattgtcatg ttacctttta aatgactgca aactctcccc tacaacgtat
      421 tggacaagaa aaaggtatcg ctatgggaag ccaggaactc caacgcaagc tcggattttg
      481 ggccgttctt gcaatcgccg tcgggacaac cgtcggctcc ggtatttttg tatctgtggg
      541 tgaagtggca aaagcagcgg gcacgccgtg gcttacggtg ctcgcgtttg tcattggcgg
      601 gttaattgtg atcccgcaaa tgtgcgtcta tgcggaacta tccaccgctt atccggaaaa
      661 tggcgcagat tatgtttatc tgaaaaatgc cggaagccga ccgctggctt tcctctccgg
      721 ctgggccagc ttctgggcca acgatgcgcc gtcattgtcg attatggcgc tggcgattgt
      781 cagcaatctt ggctttttaa cgcctatcga tccgttgctc ggtaaattta tcgccgccgg
      841 attaattatc gcctttatgt tgctacacct gcgctccgtt gaaggcggcg cagcgtttca
      901 gacgctaatt accatcgcca aaattatccc gttcactatc gtcattggcc ttgggatctt
      961 ctggtttaaa gcggagaatt ttgccgcccc taccaccact gcgattggcg caacgggcag
     1021 ctttatggcg ctgctggcgg ggatctctgc caccagttgg tcgtataccg gcatggcctc
     1081 tatctgttat atgaccggcg aaattaaaaa ccccggaaaa accatgccac gagcgctgat
     1141 tggttcctgt ctgctggttc tggtgctcta caccctgctg gcgctggtga tttccggcct
     1201 gatgcccttc gacaaactcg ccaattctga aacgccgatt tccgacgccc tgacctggat
     1261 ccccgcactc ggcagcaccg ctgggatctt tgttgccatc acggcgatga tcgtcattct
     1321 tggttcgctt tccagctgcg tgatgtacca gccgcggctg gaatacgcga tggcgaaaga
     1381 caacctgttc tttaaatgct tcggccatgt gcatccgaaa tacaacacgc cggatgtctc
     1441 catcatcctg caaggggcgc tggggatctt cttcatcttc gtttccgatc tcaccagcct
     1501 gctgggttat ttcaccctgg tgatgtgttt caaaaatacc ctcaccttcg gctccatcat
     1561 ctggtgtcgt aaacgcgacg attacaaacc gctgtggcgt actccggctt tcgggctgat
     1621 gaccaccctc gccattgcgt caagcctcat tctggtcgcc tcaacctttg tctgggcacc
     1681 gattcccggc cttatctgcg ccgtcatcgt tattgctact ggtctgcctg cttacgcctt
     1741 ctgggcgaag cgtagccgcc agctcaacgc tttgtcgtaa tgttatctgg agaaaataaa
     1801 atgttggata ttgataaaag caccgtggac tttctggtca ccgaaaatat ggttcaggaa
     1861 gtggaaaaag ttctcagcca tgacgttccg ctggtgcacg ccatcgtgga agagatggtg
     1921 aagcgcgaca ttgatcgtat ttatttcgtt gcctgcggct cgccactcaa cgcggcgcaa
     1981 acggcgaaac atctggcgga tcgcttttcc gatcttcagg tctacgccat ttccggctgg
     2041 gagttctgcg ataacacccc gtatcgcctc gacgatcgtt gcgcggtgat tggcgtttct
     2101 gactacggta aaacggaaga ggtaatcaaa gcgctggagc tgggccgggc ctgcggcgca
     2161 ctcactgcgg cgttcaccaa acgcgcggac agcccgatta cctcggcggc ggaatttagc
     2221 attgattatc aggccgactg tatctgggaa attcacctgc tgctctgcta cagcgtggtg
     2281 ctggagatga tcacccgcct cgcgccgaac gaggaaatcg gcaagatcaa aaacgatctc
     2341 aagcagttgc cgaatgcgct cggtcatctg gtacgcacct gggaagaaaa aggccgccag
     2401 cttggtgaac tggccagcca gtggccgatg atttataccg ttgctgcggg tccgctgcgt
     2461 ccgctgggtt acaaagaagg cattgtaacg ctgatggaat ttacctggac gcacggctgc
     2521 gtgattgaga gcggagagtt ccgccatggc ccgctggaga ttgtcgaacc gggcgttccg
     2581 ttcctgttcc tgctcggcaa tgatgaaagt cgccacacca ccgaacgcgc cattaacttt
     2641 gttaaacagc gtactgacaa cgtgatcgtc atcgattacg ccgaaatttc gcaagggctg
     2701 cacccgtggc tggcaccgtt cctgatgttc gtgccaatgg agtggctctg ctactacctg
     2761 tctatttaca aagatcacaa cccggatgaa cgccgctatt acggtggtct ggtggaatat
     2821 taatccctct cccggcccgg caacgggccg aatttacgca aggagttacc cgatgaaaac
     2881 aggtatgttt acctgcggcc accagcggct gcctattgaa cacgcatttc gtgatgcaag
     2941 cgagctgggt tacgacggca tcgaaatttg gggcggtcgc ccgcacgcgt tcgcgccgga
     3001 cttaaaagcg ggcggcatca aacaaatcaa ggcgctggcg cagacgtatc agatgccgat
     3061 tatcggctat acgccagaaa ccaacggcta tccgtataac atgatgctgg gcgatgaaca
     3121 tatgcgtcgc gaaagcctcg acatgatcaa gctggcgatg gatatggcaa aagagatgaa
     3181 cgcgggttat acgctgattt ccgcggccca cgcgggctat ctcacgccac ctaatgttat
     3241 ctggggacgg ctggcagaga acctaagcga actgtgtgag tacgcggaaa acatcggcat
     3301 ggatctaatc ctcgaaccgt taacgccgta tgaatcgaac gtcgtgtgta atgctaatga
     3361 tgtgcttcat gcgctggcgc tggtgccttc gccgcgcctg ttcagcatgg tcgacatttg
     3421 cgcgccgtat gtccaggcgg aaccggtgat gagttatttc gacaaactgg gcgataaatt
     3481 acgtcatctg catattgtcg acagcgacgg ggccagcgac acgcattaca ttcctggaga
     3541 aggcaaaatg ccgctgaggg aactgatgcg cgatattatc gaccggggct atgagggtta
     3601 ctgtacggtg gagctggtga cgatgtatat gaacgagccc agactctatg cccgccaggc
     3661 gctggaacgc tttcgcgcgc tgctgccgga ggatgagaga tgaaaaccct ggcgacaatc
     3721 ggcgataact gcgtcgatat ctacccgcaa ctgaataaag cgttttctgg cggtaatgcg
     3781 gtcaatgtgg cggtgtactg cactcgctac ggcatacagc cgggatgcat tacctgggtg
     3841 ggtgacgatg actacggcac aaagctgaag caggatctcg cccgcatggg cgtcgatatc
     3901 agccatgtcc atacgaaaca cggcgttacc gcacaaactc aggtggaact gcacgacaat
     3961 gatcgcgttt ttggcgacta caccgaaggc gtgatggccg actttgccct gagtgaagag
     4021 gattacgcct ggctggcgca gtatgacatt gtgcacgcgg caatctgggg acatgcggaa
     4081 gacgcattcc cacagctgca cgctgcgggc aaacttaccg ctttcgactt ctccgacaag
     4141 tgggacagcc cgctctggca gacactggtg ccgcatctcg attttgcctt tgcctccgca
     4201 ccgcaagaag acgaaacgct gcgtctgaag atgaaagcga ttgttgcccg tggcgcagga
     4261 acagtgattg tcacgctggg tgaaaacggc agcattgcct gggatggcgc gcagttctgg
     4321 cgtcaggctc ctgaaccggt gacggttatc gacaccatgg gtgccggaga ttcgttcatt
     4381 gccggattcc tttgcggctg gtctgcgggg atgacattac cgcaggcgat ggcgcaggga
     4441 acggcgtgcg cggcgaaaac cattcagtac cacggtgcct ggtaggtata acgttggcgt
     4501 gagcatcttc acgccaacgt gctgttactt gccggaaaac gaccctataa tccgagtaat
     4561 tcattcttta tttcagggtc gattatgtca gctacggacc gctactctca tcaactcctc
     4621 tacgctaccg tccgccagcg actgctggat gatatcgcgc agggggttta ccaggccggg
     4681 caacagatcc ctaccgaaaa cgagctttgt acacaatata acgtcagccg cattaccatt
     4741 cgcaaagcca tcagcgactt agtggcagac ggcgtactga tccgctggca gggaaaaggc
     4801 acctttgtac aaagccagaa agttgaaaac gccctgctta ctgtcagtgg ttttaccgat
     4861 tttggcgtct cacaaggcaa ggcgacgaaa gagaaagtga tcgaacagga acgggtcagc
     4921 gccgcgccgt tttgcgaaaa gctgaacatc cccggaaaca gcgaagtgtt ccatctctgc
     4981 cgggtgatgt atctcgataa agagccgctg tttattgata gttcatggat cccgctgtcg
     5041 cgttatcctg actttgatga gatttacgtc gaaggaagct ccacctatca gttatttcag
     5101 gagcgttttg acacgcgagt ggtcagcgac aaaaagacca tcgatatctt tgccgccacc
     5161 cgcccgcagg caaaatggct gaaatgcgaa ctgggcgaac cgttgtttcg catcagcaaa
     5221 atcgcctttg atcagaatga caaaccggtg cacgtctccg aactcttctg ccgcgccaat
     5281 cgcatcacct taactattga taataaaaga cattaaccgt aggccggata agatgcgcca
     5341 gcatcgcatc cggcgatgct ggcgcgttga attttacatc ccgtacgttc ccctcaccct
     5401 aaccctctcc ccaaaggggc gaggggaccg ttcagtacgg aatttagctt gacggtgcta
     5461 tccagcatta aatactggca atactctcac gcaaaatccg cagcaccgtc tcttcaccgc
     5521 tgcgattcgg gttaatgcga atcgcacaat gttctgattg tggattcgcc tggcgaaacg
     5581 ttccggaaag gcgataaaag agcggcggga tttcatattt cgactctgca cccaccgggt
     5641 aaggcaaggc accgcgcttt tgcgcctctt ccagcactct ggcggcaatc ggctgatgaa
     5701 actcgacaat caacaccttc gactgcgcat tagcaatcac cgcgcttttc acttccggca
     5761 ccgcaccacc gttaagcaaa gccagcaacc gttcagatac ccctgcctgc accgcgtgca
     5821 tcactggcgc aaacaccaga ccacgcaata cttccagcgc ctgtgcgccc tggatctggc
     5881 taccgccgga gtaaagcgtg gcgcgaatac ggttgataac atcagcatcg ccgaccactg
     5941 caccaacacc ctctggccca aatagcttga agcaggaaaa tgtcgagaca ttcgcgccgc
     6001 attcacagcc gattcgcgcc accttcatca ccgcatagtt gtcatcggtt aacgctggaa
     6061 cacctgccgc gcgcaacgtt gccagcacat ctgccagcac gtagctgtcc tgcggctgct
     6121 ggcgcgtatg ctgcaccagc gccgcatccg gttgttgcgc gtcgacgacc tgcttcagtg
     6181 ccgacaggtc attgaaatca acagtaataa gcgtcagccc catctgctca ataataaccc
     6241 gtgtcgtcgg gtaaacaggc gcgtcatgca ccagaagacg ctgccccggt ttgagcaaag
     6301 ccgccagccc ggcgcgaatc gcgccagtcc ccgcgccctg caccagcgcc gcagcctgtg
     6361 cgtgaaatgc atcagccagc acctgttcca cacgctgggt aacgcgcggt tgattcagtc
     6421 ctggcgttaa gcctaaatca ccgccagtaa gaaactcgct gccggggaaa tggcgacaaa
     6481 tgctatccac cagcgcaaac tgcttttgct gcgcctcaat aatcgtcagg ctttgcagag
     6541 gaaacgtctt cataggtgcc ttccttaagc cggaacgaac aggccaagcc agtaaagaac
     6601 attcagcaga ataccggtga tcattactgc cacgaccggt gccgccattt tctgtaccgg
     6661 acggcccagg gattcgttga ggaagtaaat ggcaaccgcg atagagaatc cggtataacc
     6721 cgccatctta attgctgcga aaatcgaacc gaccagcagc gccacttcca tcagcatatt
     6781 catggcgtta cggatgttat ccgacgcatt acgcaccgac gggtagcgtc ccagccattt
     6841 gccgatcgaa cgaagcagca agacttccgc cgaaataacc actgcgccta ataccgctgc
     6901 aaccatcgga ttcggcgaga gatagcccac cgcataaaca aaggtaaagc ccgcaactgc
     6961 atacacaccc gttgctaagg cggtggtggc aatcaacggt acaaaaccca gcccgcgcat
     7021 aaattctgcc agtgccgcct ggttgatcag cgtttgcgat tgttccggcg ttacgcctgc
     7081 ggagtacgct ttctccagag tgaagatcga cacttcactg ccggcgaaaa tcttcatgct
     7141 ggcaacggcg gcaatcaatg ctcccacgat ggcgatatag ggtaagtttt tgataatccg
     7201 cgacgtgcgt tcttcaaaca ccgaaagccc gctggcatca atatcatttt catcacgatg
     7261 acgcaggtcg tgagttatcg cgatccccag cagcatcacc atgccaataa agatttcgat
     7321 ggattcaggg ttaagatgtg ggaaatagcg cacgactacc acacgggtca tcagaacaac
     7381 cacggcggcg accagacttt gcttccagcc aaactggtag aaaatcgcca ccagcgggaa
     7441 cagtgcaaaa gcagaaacca ccggcgagct taattccccg aggctaccta atacatccac
     7501 cggcagcgcg gtcagcagct ggtttactgg caacaggcaa gtaaggatca acacgcccca
     7561 gatagcgcca agaccaaacg ccatcaggct gtttatcgcc agtacgccga ggatatcggt
     7621 aggaagaaag agtaaccatg cgttgagcag cccggttttc agggtaaaag agatccccac
     7681 cgaggccaca aaaccaatgc tcagaccaaa agcgatactc cccgcctcgc gacgattcat
     7741 atagccttca atcagttgcg gcaggatcgg gcggatgccg tcatgaaaaa cagccgccga
     7801 gcgatgcgcc agaagcgatg tcatacccgt caggcacgcc accacgataa tctgaatata
     7861 cagatccata gtcgtgcctt ttatttcaga tggttaatca gcatcggaat cgcgtgttcg
     7921 acgtgctcaa cggaaaggcc aaacgccact ttcccttcag cgatcatttt ggcgatatgc
     7981 tcgtctttcg ctttaatgcc tggtttggca atggtgcaac ttttgttata gccaatcacc
     8041 gcgatggcaa tcgacaatgc cgcgcccgca ccggtattac acgcaccgat gtagtaatcc
     8101 agttgcccgg atttcacttt cattgccgct tccatgtcgt tgtgaatgaa aacttcaaaa
     8161 ctgccaggag ccgtcgcttc aatgtttttt ttaatctgct cacgctgtaa gcctgcaacg
     8221 ccaatctttt tcatgagtct gtccttattg gaaaaattga gagggatttt cacgtaacat
     8281 cacatccaca tcggcctgac tgaatcctga ctggcgcaat tgcggaataa aggtggtcaa
     8341 taaatagtca tagccataac caccattggc ttttaaatgg gagcggcgcg taatatccat
     8401 cgacagcatg acgcggttca gcaacccacg gtcacgtagc gcgtggagca tcgcaatgcg
     8461 cttttcgtcc gggtagtaac tgttcttacc aatggtgtcg aactgcacgt acgcgccgag
     8521 atcgatcatc ttcaaaatgt tgtcgagatt gtctttcaga tcgcagtgac caacggtgac
     8581 gcgcgaaaga tcaactccgt gggcttgcaa cagagccagt tgttccagcc ccatggtgct
     8641 gaacgaagta tgcgtggaga tcgggcgtcc ggtctggtta tgcgccagcg cagcggcaat
     8701 aaacaccttc tcttccagcg gcgtaatctt cccttcgcta gagccgatct ccgcgataat
     8761 cccggctttc aggtcggtgc catcgatacc ctgttcaatt tcatcgacca tctcctgcgc
     8821 cagttcctgc acgctgcggg tcgccacatg ctccgggaaa aaagcgtcct ggtaataacc
     8881 ggtgcaggcc accacgttga tccccgtctc gcgcattaca tcgagcataa attgcgcatt
     8941 gcgccccatg taacggttgg tcatctcaat cacattacgc acgccccggg tcatcaggtc
     9001 gttcatctcc tggcaaatga acgcatactg atcaaggcgg cagtccacgt tgtttttaaa
     9061 gccggagaga tcaatatgca gatgctcatg ggccagggtg taacccgtcg gatcaatact
     9121 catagggtgt ctcctgcaaa ccggagggag gaaagaaaag agcgaccatt ttgtggcggt
     9181 ggcgcacgga aaaattcaca cacggtagcc cccacatcag aaagcgtggt gcgtacaccg
     9241 agttgcgtgt ggaccagccc ttgctgataa accagcactg gcaccacttc acgggtatgg
     9301 tggctgtgac caatggtcgg atcgttaccg tgatccgcca tcacgaccag gcaatcatct
     9361 ggctgcatcg cctcaacaag ccgggcaagg ttacggtcaa cgacctgcaa acgttcggca
     9421 taacgtgcga cgtcttctgc atgaccagcg aggtcggttt cctggatatt gatgcaaata
     9481 aacgccgtcg gatgcgtgtt aaattcgtcg agggtgatat ccataatccg ctggctatcc
     9541 accaggtttt gccagctcac gccataagga ttgctgacga tatctgccac cttacctacc
     9601 agcacggttg gcacgcccgc ctcatacagt ttttgcggca cctgcacttt ttcatcaaca
     9661 ccgtagccca tatgcacgac ctggaagccg ttgtcataag cgccagaacg cggcgcatta
     9721 ataccaataa agcgcccttc tttgctttct gcggcatcga gaatacgctg actgtcggtt
     9781 aacaagccgc caaatgtaat gacccgaccg acctgtacct gctcacgcac gatacgacca
     9841 attttgatcg cgtcgtcaaa agagatcaca gagagattgg cggtaatgtt atagacctgg
     9901 cctaaatccg cctcgagatt atcgccaatc gcaaccgcct gattgaccca cagaaattgc
     9961 agatcatcgc cacggcgctc cacctgccag ccagcggaaa ctaatgcctg ctcaacacgg
    10021 ccaatcacat cgcgaaaggg catccgcagc ggcggtaacg ggcgcgtgcc taaaatttcc
    10081 tgatgcccca taaaggtatc gccaccttca tgttgcagct ctgccacgcc ccaggttgcg
    10141 gaatctgacg gctgcatatc gcctggcgca taacccaatg cgttgattag ccccagcttc
    10201 tccagcgttg gtagctgcaa atgcggcaac tggctcagga tgtgaccaca ggtattcgcc
    10261 ccggcatctt gcggacgcac cagcgtgaca tctttcattg cccctacgcc aaagctatca
    10321 atcactaaca ccacaaatcg cgccattacg cccccagaga gtttccaaga ctgtcataac
    10381 gaccaacgat tttcggttcg ccacggtgaa taccggacac cagcaccaca tcgctacggg
    10441 tgacaaaaat ctgcgtgcgg aaacagagca ccactgcact gctcaccgga tactcgcctg
    10501 ccagcggcag ggtgtagtcg atactgctgt catccacagt tttgagattg gtttcagtaa
    10561 tcttttgatt ttctggcgta aacaccagcg catgttgcgc atgaccacga cggtagtaac
    10621 cgccgccgta gcagtagctg tcgccacgga aatgatggga gatttcactt aaccagagca
    10681 tcgcgatacg ttcaggctga tcgccctgct ggtttgccgg aatagtgccc gtcagcgcat
    10741 gaccgggttc ggcatgagtc acaccgtatt ccgccagcaa tggcagcgaa gtgcagctgg
    10801 tcgctgaagg cgcgttcagt tgctcaagtg caataccaga tttcgccagt tgatcccgtg
    10861 cctgtatcag cgtgtgaaga ttcggtgtcg gcaaaacttt tccggcagcc tcatcccaaa
    10921 gcaggcaagg gaaatgggta agtccggcta aatgcagccc cggcagtttc ttaatttcag
    10981 caaccacctc gtgcagcgaa tgctgtacaa aaccgctctc ctgccccgga taaagaaaat
    11041 catcgtcgct ataaattttg agcagcacaa actgcacacg tccggccttc accgctgccg
    11101 cagaaacttc ccgcgctttg tcgagagtaa acacggtgat gacgtcggtg ccctgttcaa
    11161 cggcgtcagc aacctgatga caagggattt gtaccagatg cccctgatgc gccaccgaca
    11221 aaccagcgcg gcgcatgact cgcgcctctt tgtaatccac cgccacaatg ccgctgtaac
    11281 ctaatgccag caatttttcc gccagccatg ggttacggcc aaactgcttg gtcatcagat
    11341 acagttcaat gccgtaaagc cgcgccgtct caatcagtcg cttaccgttt tccagtacct
    11401 gatccacgtc gatcacccag ctgtccgggg cgatcttgcc ctgctgccac aaacttagtg
    11461 cggcggaaat cagcgccggg ttctggcgtt tcaatgcttc tacaaacatc ttgttgcctc
    11521 gtttctgttc cttgaattaa atattcattt ttttgaatat ttaggttgtg cgacgtggat
    11581 ttgtcgggag cttcaaacct cttcgttagc agccatccat aatccataaa ggttggccag
    11641 caaatagcct tcttcacacg ggttaacttc cagtgcgaat tccttcaaca acacctgatg
    11701 caattgcaca acggcttgcc agtggctgga ttgcgccagc tctgccagca attcgttatc
    11761 cagcggctct atttcttcac cacggcgact gcgcatcagt gcactcgcca tatgtgtcat
    11821 cgccatcgtt ccttgctcac tgcgcaccgg cagatggcac tctgtttcca gtacgttgac
    11881 gacctgcatc atgcctttgc agatgtcctt atcaataacg cctgcctcgc aaagcaggtt
    11941 gagtctggtt tccatatcag atgcctttcg tttaataact cggttcctgt tccccactca
    12001 ctacccgttg ttgatgggcg agcagggcgt gtttatctac aacggcacgc agcagttgtt
    12061 gcattgggta atcatcgact cgcgtcagga ccacggcttc ggtggcctgt aaaaatcgcg
    12121 gatcgtctgt gagcggcgtc gcctctaatc ccagcatggt cagttcgttt tccgccacca
    12181 cgttccagat caccgcatcg acatcgcctt taacaatgcg ttgtaaactc tcgtgataag
    12241 agagatcgac tcgttccaca tcactaccgc caaaaaaaac atcggtcatg attttctgat
    12301 ccgccgaacg gttatccagc cccacgcgct tcacgtttgc ggactcgcct ttacggcaaa
    12361 tcaactggtg ctcgccaacg taggtgtgcg gccccaactc cagcgcgagg cataaccctt
    12421 tttgcgtgag ataactttcc gccgccagtc gggaaaccac cgccatgtca tacacgccgt
    12481 taagtaaaca ctccacgcga atatccgcgc cgcgcatatg cgcatagtaa aaaggaatgc
    12541 catcaaactg agctttcagc ccgctcgcta aaccttcgta caagcgggta tagggcaaag
    12601 gcatcgcaca taccacgttg ttgatatcca catgactcag caatgctttg ttatccatct
    12661 cgaccagata actgccattg cgcccacggc gttcaatccg tatcgctccg cttgattcca
    12721 gcgttttcaa cgcggcctgc gtcaggccaa ccgatgaacg gcattcagtt gccagctcat
    12781 ctatggtttt caacctatta ccgcattttt cacccaacaa ataacgggcc agcgtcgtga
    12841 taacgacgcc ttcttttttg ataaacgttc gacgcataat atattttcaa taaaatgaat
    12901 gtttatatct tcattaaaat gaagataagt ggcaactgtg agatgtggaa acggcgaggc
    12961 gcttcacatt ttttcggatt attgcaaatg aggataaaaa aaccgggttt ccccggtttc
    13021 agagtgatga cgaagtgatt tattcgacaa tgcgagcaaa gggtaattcc cactggcgct
    13081 tgcctgctcc cctcacccta accctctccc caaaggggcg aggggaccgt ccgagcgttt
    13141 tgatattaag gcttcgccac aaaaccaatc gcttcgtaca ccgctttcag cgtacgggaa
    13201 gcgtgtacac tggctttttc tgcgccgtct ttcatcacct gttgcaggaa ggcttcatcg
    13261 ttgcggaaac ggtgatagcg ttcctgcaat tcagtcagca taccggaaac ggcatcagcc
    13321 acttcacctt tcagatgacc atacatcttg ccttcgaact gtttttccag ttctgggatg
    13381 ctctggcccg ttaccgcaga aaggatatcc agcaggttgg aaacgcccgc tttgttctgc
    13441 acatcgtagc gaactaccgg cggctcgtcg gagtcagtga ccgcgcgttt gattttcttc
    13501 actaccgatt tcggatcttc cagcaggccg ataacgttat tgcggttatc gtcagacttg
    13561 gacatcttct tggtcggctc cagcagcgac attacgcgcg cgccagattt cggaataaac
    13621 ggctccggca ccttaaagat ctcgccatac agcgcgttga aacgctgggc gatatcgcga
    13681 ctcagttcca ggtgctgttt ctggtcttca cccaccggta ccagattagt ttgatacagc
    13741 aggatgtccg cagccatcag caccggatag tcaaacagac cagcgttgat gttctcggca
    13801 taacgcgcag atttatcttt aaactgggtc atgcggctca gttcgccgaa gtaggtatag
    13861 cagttcagtg cccagcctaa ctgcgcatgt tctggtacgt gggactgaac aaaaatggtg
    13921 cttttctcag gatcgatacc acaagccaga tacaaggcca gcgtatccag cgtcgcttta
    13981 cgcagcttct gtgcatcctg gcgcacggtg atcgcgtgtt ggtcaacgat acagtaaatg
    14041 caatggtagt catcctgcat gtttacccac tgacgcagcg cacccatgta gttaccaatg
    14101 gtcaattcac ctgagggctg tgcgccacta aaaacgatgg gcttagtcat ttttcgattc
    14161 ctgattttcg ctatgcggaa gccctaatgc gggcagaagg tcatttatag actgataaat
    14221 tacatcaggc tggctgagat cgatagcctc gccgtagtta tatccgtagg ttaagccaac
    14281 tgatgggcaa cctgccgctt ttgccgcctg aatatcattg cgtgagtcgc cgacaaacag
    14341 catctgttgt ggggcaattc ccatccgctc agccaccagt aacagcgggt ccggatgcgg
    14401 ttttttgttt tgcacatcat cgccgccgat aaccacgcta aagtatttgg caatatctaa
    14461 ggcttcgagc agcggcgcga cgaacggcgt cggtttgttg gtgaccaggc ctagcggcag
    14521 gcctttagcc tgcaacgcgc ccaacgtatc ggcaacgtgc gggaacaaaa acgtcccctc
    14581 ttcggcaacc tcgctatagt agcgatcgaa cagtttacgc agaatacgta cctgttcttc
    14641 tgccggaatg tcgtcatcaa cgggcggttt acccattgtt ttacgctgag tcgcacgttc
    14701 ctgacgcgcc caggtcaatg cgcgctccat cagaacatct gcgccgttac caatccaggt
    14761 aataacgcgt tcttcacctg cgatgggcaa ctccagcgca tacagcgcca tatctaccgc
    14821 agcagcaaga ccgggagcac tgtcgaccag cgtaccatca agatcaaaag cgacgccgcg
    14881 aatatcttca aacttattca tgacttacct ttgccagttc actgcgcatt tcatcaatga
    14941 cttttttgta gtctggctgg tcgaagattg ccgaaccggc gacgaacata tccgcgcccg
    15001 ccgcagcgat ttcgccaatg ttgttcacct tcacgccacc gtctacttcc agacgaatgt
    15061 caaagccaga ctcgtcgata cgacgacgta cttcgcgcag tttatccagt gtttgaggaa
    15121 tgaacgactg accgccgaaa ccaggattga cggacatcag caggatcaca tccagcttat
    15181 ccatcacgta atccagatag ctcagcggtg tcgccgggtt aaataccagt cccgctttac
    15241 agccattttc tttaatcagt tgcagcgtgc ggtcaacatg ctcggaggct tctggatgaa
    15301 aggtaatgat gctggcacca gcggcagcga aatcaggcac aatgcgatcg acaggtttca
    15361 ccatcaggtg tacgtcgata ggggcggtaa tgccgtagtt acgcaaggat ttcagcacca
    15421 ttggcccaat cgtcagattg ggaacatagt ggttatccat gacgtcaaaa tgcacgacat
    15481 cagcgccagc tgccagggct tttgcggtat cttcacccag gcgggcaaaa tcagccgaca
    15541 gaattgaggg ggcaatcaaa tactgtttca tccgcttctc cttgagaatt attttttcgc
    15601 gggtgaaacg actcctggtt tgtacaaagc cagcagttcg tccacctttt tacgtgtgcc
    15661 gccgttgctg cttatactgc gtcgaacttt gacgacatgc aattttgcgc gctgatacca
    15721 ctcacgcgtt aacatcgtat cgtgattgga gatcagcaca ggaatatggc gatcaaccag
    15781 accttcggcg atctccgcca gatgcgcttg ttgttcaagc gtaaaactgt ttgtgtgata
    15841 cgccgtaaag ttggcggtcg cagacagcgg tgcataaggc ggatcgcaat agacgacgga
    15901 tgcatcatct gcgcgcgcca tgctatcggc gtaagactca caatagaaaa aggcattctg
    15961 cgctttttca gcgaagtgat acaactctgc ttccgggaaa tagggttttt tgtagcggcc
    16021 aaacggcacg ttaaactcac cgcgcagatt gtaacgacac aggccgttgt aaccgtagcg
    16081 gttcaaatat aaaaacagta ccgcccgacg gaacggatcc tggcttttgt tgaactcttc
    16141 gcggaactga tagtaaacct cggcgcaatt tgtttcggga acaaacagct cgcgtgcggc
    16201 ctgtacgtac tcatcagtac gcatcttcac aatgttatag agactgatca ggtcgctatt
    16261 gatatcggca aggatataac gagaaaagtc ggtgttgaga aacaccgacc cggcacctac
    16321 aaaaggctca accagacatt cgcccttggg caaatgccgt ttaatatcat caagcagggg
    16381 atacttgccc cctgcccact tcaaaaaagc gcgatttttc ttcatgctga ctaactaatt
    16441 acaccttctc cggctgtgga gaaagctccg acagcatcca gcgcttcagg caactcccgc
    16501 aacgataaac cattgcggga gataaccttg attacttcag atcggcctgt acctgacgca
    16561 gcggtttcgc ccacgggttt ttggcctgaa catctgctgg caatgtagat accgcttttt
    16621 tcgcctcttc tttcgaagcg tacacgccag aaaccaggac ataccacggc tgaccattac
    16681 gcgtcgtttc atagacaacg tagtttttca gattctcttt cttcgcccaa ccgttcaggt
    16741 tgtcgtagtt agaggaactg ctcagctgca gagtgtaatg gctggacggt gccgatttca
    16801 acgaaccaac attacctgcg gtcttccctc cagcggctgg tgttgccgtg gtttgcgccg
    16861 gggatgccgt ccgtactgga gccgtagtcg ccgtctcttt tggtgctggc gtagaagtcg
    16921 cagccggtgc cttcgtgctc gccacagggg cagctggttc agtacgcttc ggcgtctgtg
    16981 ctaccggctt cggctccgtt ttcacggttg cttgcggttt tttcggttca atcactgcct
    17041 gctggcgagc tggacgcgta gtggccggac gttcagcggt ttgcgttttc gccgtttcac
    17101 gggatgcatt gccattgcga acaggcgcga cagtcgcggg ttcggtcggc aatgtggaat
    17161 tgaccgccac attgttcaac tgttgctgat tttgtggctg ggtcagcgca ttgttcaggt
    17221 caccctgcac ttcaacacgt tgttgaccat ccgttgccgc cggggtttgc ccttgagtcg
    17281 gcgtagaaga gatcggtggc agagaaacat cctgctgagt attttccgca gacgtggttc
    17341 ccggcgctgg ctgcacgcca ttcgcctgat cggtcgcatt gccagcaaga tcaatactct
    17401 tctcgccaga cgcggtttga tcgctggaag tggtcgaggg ggcttttagc gcagaaccga
    17461 taccgatgat caacagcagt agaaccagaa tgccgacgcc catcatcata tactgacgag
    17521 aagcgggttt actggctgct tttttgcgct tacgcggacg acgctctacg cgctcttcat
    17581 ccacggtttc atcttcggat tcgtcaattt cttcttcgat ttccggttcc tcattgcgct
    17641 ctttttgcgc acgagtcgga cggcgatcgt cagtgtcatc aagttcaata tcatcaaaat
    17701 tgatctgcgg ttcgccacgt tcagtacgct cagaacgttc agaagattga cgagaacgac
    17761 cagtacgacg atcgctggga tcgggtttca gctcgtcttc tggtttgaat tcatccattt
    17821 aacaccccac taaaaggtta atgcttacca cgttgcaatt aacctgaagc taatagaccg
    17881 cttgataagc ggcctgacct ttcttgttgt tacgctgatt gacaatcggc aatggcgtta
    17941 agaacaagct cgtgcgaaac gccgctgcga acttcactct taccaattgc caacggaaga
    18001 attaagcgca tctctcccgc aaggactttc ttgtcacgca gcatatgcgg taaatacgcc
    18061 tgcgcggaca tttcgcgcgg cccattgacc ggtaacccag cccgcgtgag cagggttata
    18121 atacgctgcg tttcggcaga actaaactgc ccgagacgtt ccgacgtccg cgccgccatc
    18181 accatacccg cagcgaccgc ttcaccatgt aaccaattgc cataccccat ttcagcttca
    18241 atggcatgac caaaggtgtg tcccagattc agtaaagcac gtaacccggt ttcgcgctcg
    18301 tcggcggcga caacttctgc cttcagttca caacaacggc gaatacagta cgccattgcc
    18361 ggaccgtcca gacgcaacaa cgcatccaga ttctcttcca gccagttaaa aaacgcaccg
    18421 tcaagaataa tgccgtattt gatgacttct gccagccccg acgctaactc acgcggggga
    18481 agcgttttca gacagtcgag atccaccacc actgaagcag gctggtagaa cgcgccaatc
    18541 atgtttttac cgaggggatg gttgaccgca gttttgccgc caacggagga atcgacctgc
    18601 gacagtaacg tcgtcgggac ttgaatgaaa cgaacaccgc gctgataact cgccgccgcg
    18661 aagccggtca gatcgcccac tacgccgccg ccaagcgcca ccagcgtagt atcgcgacca
    18721 tgcggctttt gtaacaacgc cgtaaagacg gtatcgagta cagccaggct tttatactgc
    18781 tcgccgtcag ggaggataac gctatcgacg ttaacacccg cctgttcaag tacgccgcgg
    18841 accttatcga gatacagagg agccagggtt tcgttggtga ccaacatgac ctgctcgccc
    18901 gatttcagcg gtaagaatga agctggttca ttaaacaaac cagatgcgat ggtaattggg
    18961 taactacgtt ccccgagagt aacgacaatc ctctccataa cgcgacatcc accttaatta
    19021 ctgtacccgc agacgagtgt atataaagcc agaattagtt gctttccagc atgtgaataa
    19081 tctggtttgc aaccacttta gcgctttgat catcagtacg aatggtcacg tcggcaatct
    19141 cttcatacag cggattgcgt tcattggcca acgcttccag aacttcacgc ggcggtgttt
    19201 caacgtgcag caacgggcgt tttttatcac gctgcgtgcg tgcaagttgc ttttcgatgg
    19261 tcgtttcaag ataaacgaca acgccacgag cggaaagacg gttacgcgtt tcacgggatt
    19321 tcacagagcc gccgccagta gccagcacaa taccctgttt ctcggtcaac tcattgatga
    19381 ccttttcttc gcgatcgcgg aagccttctt cgccttctaa atcgaaaacc cagcccacat
    19441 cagctccggt tcgtttctca atctcttgat cggaatcgta aaattccata ttgagttgtt
    19501 gagctaactg gcgcccaata gtgctttttc cggcacccat aggcccaacc agaaagatat
    19561 tgcgtttctc tgccattttt tcggtactac taagactatt cgttaatgat aaacccgctt
    19621 cgctcagaga gcgccgcagg acatgaactg aaacctcata agatattgcg agagtcagac
    19681 tgaaaattat ctcaatactc cagcgggttt ggcaactgaa taaatcaccc atccctctgc
    19741 atatctggtc gctgcaagcg cgctgccttg ctaccaccgc tctggcgata aatcaccggg
    19801 taagattagc gtaaaaaaga cagcaaaatg ccgcctgaat gataaatcat catcatggaa
    19861 cgcaggcagc gaagagcacc aactctcaaa tcggtactcc ttgtatgcta aatacctgcg
    19921 cgcgtcaaat agatgaaaca cgttcagcat aaaaacatta ccgcttttac ggctgtttac
    19981 tcactggaaa ccagtcgtgg cgtgataaac accactaact cgcgtcgttc atcttctttt
    20041 ccgtcatgac gaaataattg cccgaaccag ggaatgtcgc caagcaacgg tacgctatcc
    20101 tgacccgatt tatttttacg ggtaaaaatg ccgcccagcg ccaacgtttc tccgcttttg
    20161 acctcgacct gcgtttcgat ctcctgctta tcaatcgcca gcacttcgcc atcggcctgc
    20221 tgtagcacct gccccggaac gttctggctg atgtgtaatt tcagccggat gcgacctttt
    20281 tgtaacaccg tgggcgtgac ctccatcccc aggacggcct ctttaaattc caccgacgtc
    20341 gcgccacttt ccccgctgga aacctgatat ggaatttcgc tcccctgttt aatgctggca
    20401 ggctgaagat gtgaggccag cagacgcgga ctggcgataa tatccagctg ctgtttttgt
    20461 tcgagcgcgg aaagctcaag atccagcaaa cgtccgttga tgcgcccaat gttaaaaccg
    20521 acatgcgttg tcgccgtcgc tacggagagg tcgctaccaa gcgtggtgac ttgcccaacg
    20581 ccaccagcgt gttgcgcatc ggccagcgtc catttcacgc ctaactcacg caaacttttt
    20641 tcattaatgg tgacaatatg cgccgacagc tcaacctgcc cgaccggcag atccatttgc
    20701 gctacccact gttcaagcgc gcttaacgcc gttttgttat ctcgtagcaa aaggcgattg
    20761 gtgcgtttat cgacggtcat actccctttg gcactcagta gcttctcccc cgctttcgcc
    20821 agttctcccg cgtcggcgta ttgcagggtt atactgcgat tttccagcgg cagatttgcc
    20881 tgcgcccgcg cctgctccgc ctcctggcgg gcgatattgt tattctgcca ggcaatggaa
    20941 tgcactgaga gaatgttgtc ttcctgccgc gttatcagtc cggcgctttt cactacagtt
    21001 tgtagtgcct gcttccaggg aacatctgtt agatgtaacg acaccgtacc gctgacgtct
    21061 ggcgacacga ccaggttcaa cttctcctgt tcagccagcg cctgcaacac ctgagctacc
    21121 ggaacgtcat ccaccatcag cgtcactttt tgcggctttg ccgcctgtac gccgggtatc
    21181 agcatcaaca gtagtgcggc tatccattgc ttcatttgta tctccttgcc gttgccacaa
    21241 ccattgtggc ggttcgcagt ttgtcccggt acccagcgtt agtacgtctg gcgtcagctg
    21301 taaaattgtc cagccgtttt ccagcacatc gttttgctgc acccgtcgcc atttcttttg
    21361 cccgtctttt attacaccga tgatgcgctc gcctcgccct accatcccct gatagcgcca
    21421 ctggctaagt tcgctaatcc ggcatagatc ttccggcggt ttaaaagggt cacgcatacc
    21481 ggttaaaagg cacaatgcaa tacctgccaa caaccagcgt ttaaccctca tgcggcgtct
    21541 ccagttgtag cgtgaacaaa agatcatcac cttccacgct taacgaaaaa cggctcacgc
    21601 tgacgttgcg ctctgccagc cgtgtaaatg ccgacggcac tgcttcccac agcgttttca
    21661 acgccaactc gcctccctgc gcggatggat gccaggaaac cagttgcgcg ccggataact
    21721 gaaaatccag tggcgaaaag ggcagcgttt tttcctcgct gaagggagtg gtgtctacca
    21781 ggcgatacag tgcggcccac tgctgttgat gacttgcccg tagtcgaatt aatgcgtcgc
    21841 gctcttcatg gtgtgtcgat gacagaaaaa tgagcgtaac taacatcagc aaccagaatg
    21901 cccagcaaaa ctggcggagg cggggtgatg tggcgaacca ccagtcaaag aacatgttca
    21961 tcgctaacct tccttgttaa ctgatactca aattgccagc gtccctgcgc atcctgctgc
    22021 gtggcccccc gctgattgag atgaaaagaa gcatcctggc gaagtgacgt ttctagtgcg
    22081 tttaacgcgg taatgcttgt tgtaagcccc ttgatctcca gcgttccctg ctgccagctt
    22141 atcgttgtca gccaggcgtg ttcaggtaaa agagccgcca gtgcttccag cgcagattgc
    22201 cagtcgcggg taaattgtcg ctggcgctgc ctttgcaaac gctgctcacg aatttgttgg
    22261 cgctccagca aacgtggttt cgttatctgt aagctgtggg cgagttgttg ttccccctga
    22321 agcaaaacgg cgtctacgcg agcttcggcg ctgcttgtca gacgcagtat tagcgttatc
    22381 ccgacggcca gcagcagagg cgcaacgaac atcagcaacc agaaacgcag aaaagcggtc
    22441 cggcgttgct gtcgccaggg caaaaaatta attggcaggt tcatcagtac gccttcccaa
    22501 gcgccagccc cagcgcgatg gcaaagtctc cacctggcgg cggtagcggc ggctgacgaa
    22561 cagaaacggc ctcccaggga tcaaatccgc cttcgccaca tatcgcgacg ctgtcgggat
    22621 caacggataa cgctgccgcc agctccttcg cgctagtcat ccctaccgcc agtttgcgcc
    22681 cccagcgata gcgtgtcgcc cacagccact gttcgttatc acgccaggcc agacattgct
    22741 gatgagaagg taaaaaaggc aggaattgct gtaatgcact ggcatccggg gtgatcgcac
    22801 taacatgaac acgcaacctt tctgccagcg taagcagcgt tgccagctct ttgctttgcg
    22861 cggcagtcac gttataagcg gggctgagtg agtcttcgct ataatcgaag cgcagggagt
    22921 ccggatccat atccagctcg cgggccatcg tccctgacag ccaggccgtt tgctcccgct
    22981 caccaaggga catcgacggg cgcggaaatg accgctgtaa tgtgcgactg gcgggaaacg
    23041 ccaacataat gtgatgacgc tgcggcagtt cgcgactcca aggtaacaac gttttagcca
    23101 gctgctgcgc atcaacaatc cgcccatctt tgataatgtc gttctccagc ggcaaccgcc
    23161 accagcgttg caaaaagcat tcttttgcgc cccgtacgat cgcaaccgct accgcttctt
    23221 gctgttgtaa atgcaaacca atttgccaga tcttaaatgc cattgtgatg atctccttat
    23281 cacccgtcac tctgacgggt atatcaatgc gtctggcttg cctttatact accgcgcgtt
    23341 tgtttataaa ctgcccaaat gaaactaaat gggaaatttc cagtgaagtt cgtaaagtat
    23401 tttttgatcc ttgcagtctg ttgcattctg ctgggagcag gctcgattta tggcctatac
    23461 cgctacatcg agccacaact gccggatgtg gcgacattaa aagatgttcg cctgcaaatt
    23521 ccgatgcaga tttacagcgc cgatggcgag ctgattgctc aatacggtga gaaacgtcgt
    23581 attccggtta cgttggatca aatcccaccg gagatggtga aagcctttat cgcgacggaa
    23641 gacagccgct tctacgagca tcacggcgtt gacccggtgg ggatcttccg tgcggcaagt
    23701 gtggcgctgt tctccggcca cgcgtcacaa ggggcgagta ccattaccca gcagctggcg
    23761 agaaatttct tcctcagtcc agaacgcacg ctgatgcgta agattaagga agtcttcctc
    23821 gcgattcgca ttgaacagtt gctgacgaaa gacgagatcc tcgagcttta tctgaacaag
    23881 atttacctcg gttaccgcgc ctatggtgtc ggtgctgcgg cacaagtcta tttcggtaaa
    23941 acggtcgacc aactgacgct gaacgaaatg gctgtgatcg ccgggctgcc gaaagcgcct
    24001 tccaccttta atccgctcta ctcaatggat cgtgccgtcg cgcggcgtaa cgtcgtgctg
    24061 tcgcggatgc tggatgaagg gtatatcacc caacaacagt tcgatcagac acgcactgag
    24121 gcgattaacg ctaactatca cgcgccggag attgcttttt ctgcgccgta cctgagcgaa
    24181 atggtgcgcc aggagatgta taaccgttat ggcgaaagtg cctatgaaga cggttatcgc
    24241 atttacacca ccatcacccg caaagtgcag caggccgcac agcaggcggt acgtaataac
    24301 gtgctggact acgacatgcg ccacggctat cgcggcccgg caaatgtgct gtggaaagtg
    24361 ggcgagtcgg cgtgggataa caacaagatt accgatacgc tgaaggcgct gccgacctat
    24421 ggtccgctgc tgcctgccgc agtcaccagc gccaatcctc aggaagcgac ggcaatgctg
    24481 gcggacgggt cgactgtcgc attgagtatg gacggcgttc gctgggcgcg tccttaccgt
    24541 tcggatactc agcagggacc gacgccgcgt aaagtgaccg atgttctgca aacgggtcag
    24601 caaatctggg ttcgtcaggt tggcgatgca tggtggctgg cacaagtgcc ggaagtgaac
    24661 tcggcgctgg tgtcgatcaa tccgcaaaac ggtgccgtta tggcgctggt cggtggcttt
    24721 gatttcaatc agagcaagtt taaccgcgcc acccaggcac tgcgtcaggt gggttccaac
    24781 atcaaaccgt tcctctacac cgcggcgatg gataaaggtc tgacgctggc aagtatgttg
    24841 aacgatgtgc caatttctcg ctgggatgca ggtgccggtt ctgactggca gccgaagaac
    24901 tcaccaccgc agtatgctgg tccaattcgc ttacgtcagg ggctgggtca gtcgaaaaac
    24961 gtggtgatgg tacgcgcaat gcgggcgatg ggcgtcgact acgctgcaga atatctgcaa
    25021 cgcttcggct tcccggcgca aaacattgtc cacaccgaat cgctggcgct gggttcagcg
    25081 tccttcaccc caatgcaggt ggcgcgcggc tacgcggtca tggcgaacgg tggcttcctg
    25141 gtggacccgt ggtttatcag caaaattgaa aacgatcagg gcggcgtgat tttcgaagcg
    25201 aaaccgaaag tagcctgccc ggaatgcgat attccggtga tttacggtga tacgcagaaa
    25261 tcgaacgtgc tggaaaataa cgatgttgaa gatgtcgcta tctcccgcga gcagcagaat
    25321 gtttctgtac caatgccgca gctggagcag gcaaatcagg cgttagtggc gaagactggc
    25381 gcgcaggagt acgcaccgca cgtcatcaac actccgctgg cattcctgat taagagtgct
    25441 ttgaacacca atatctttgg tgaaccaggc tggcagggta ccggctggcg tgcaggtcgt
    25501 gatttgcagc gtcgcgatat cggcgggaaa accggtacca ctaacagttc gaaagatgcg
    25561 tggttctcgg gttacggtcc gggcgttgtg acctcggtct ggattggctt tgatgatcac
    25621 cgtcgtaatc tcggtcatac aacggcttcc ggagcgatta aagatcagat ctcaggttac
    25681 gaaggcggtg ccaagagtgc ccagcctgca tgggacgctt atatgaaagc cgttcttgaa
    25741 ggtgtgccgg agcagccgct gacgccgcct ccgggtattg tgacggtgaa tatcgatcgc
    25801 agcaccgggc agttagctaa tggtggcaac agccgcgaag agtatttcat cgaaggtacg
    25861 cagccgacac aacaggcggt gcacgaggtg ggaacgacca ttatcgataa tggtgaggca
    25921 caggaattgt tctgattaaa aaggcgcttc ggcgcctttt cagttggctg acaaagtgca
    25981 tttgtttatg ccggatgcgg gtgaacgcgt tatccggcta acaaaatcgt gaaaaatcaa
    26041 tatattgcag gaaacccata agcctgataa gcgttgtgca tcaggcaaac ttcacgcatt
    26101 tacactcgcc cctgcccttt caaccattcg cgcacgagga acagcgcgct gacattacgc
    26161 gcttcgttga agtcagggtc ttccagcaaa tccatcatat gcgccagcgg ccagcgcacc
    26221 tgtggtagcg gctctggctc atcgccttcc agtgattccg ggtagagatc ttgcgctacc
    26281 acgatattca ttttgctgga aaaataagac ggtgccatgc tgagcttctt caaaaaagtc
    26341 agatcgttcg caccaaatcc aacctcttct tttagctcgc ggttagcggc ttcgtagacg
    26401 ctttcacccg gatcaattaa acctttcgaa aaacctaatt cgtaggattc agttcccact
    26461 gcgtattcgc ggatcaggat caggtgatcg tccacaatcg gcacaatcat cactgcttcc
    26521 cggttggttg gacgcattcg ttcataaaca cgccgcacgc cattgctgaa ctccagatcc
    26581 acgctctcga cggtaaacag tcgggaacgg gctacagttt caacattcag aatggtgggt
    26641 ttttgtaatg atttgctcat cgtgggatct atgctgtgaa atcagcggtt attgtgcgat
    26701 atcggacacg ctttcggcaa tgtgaattgc atgttattta catttatgta acttaataaa
    26761 taatcgtcct caaatcaaat taaaagtcaa taggttgaaa taactccagg aatttgctga
    26821 tattccgcct tcagagggtt tgctatgatc agcggttact gtgatgtgct taatgatgct
    26881 caagttaaac tccacgcttg ccgatagcca accgcagaat catgtattgt gtctggtgcg
    26941 actgaccacg cctgacagac taagtaagat ggggaaagca tgagcaccat tgtgattttt
    27001 ttagctgctt tgctggcctg ctcactactt gcgggatggc tgataaaagt gcgatccaga
    27061 cggcgtcagc tgccctggac caacgccttc gcggatgcgc aaacgcgtaa actcacacct
    27121 gaagaacgta gcgccgttga aaattatctt gagagcctga cgcaggtatt acaggtgcct
    27181 ggcccaacgg gagctagcgc ggcaccgatc tctctggcgc tgaatgccga aagcaacaac
    27241 gtcatgatgc tgacacacgc tatcacgcgt tacggcatct ctaccgacga tccgaataaa
    27301 tggcgttact acctcgattc ggtagaagtc cacctgcccc ctttctggga acaatacatc
    27361 aacgatgaga ataccgttga actgatttat accgattcgc tgccgctggt tatttcactc
    27421 aacggtcata cgctgcagga gtacatgcag gaaacgcgcg gctatgcctt gcaacctgtt
    27481 ccgtccacgc aggcgtcgat tcgcggggaa gagagtgagc aaatcgagct actcaatatt
    27541 cgcaaagaaa cgcatgaaga atatgcgctg agtcgtccgc gcgggctgcg tgaagcgttg
    27601 ctgatcgtcg cctccttcct gatgttcttt ttctgcctga ttaccccgga tgtatttgtt
    27661 ccgtggctgg caggcggcgc attactgctg ctgggcgcag gtctgtgggg gctattcgcg
    27721 cccccggcaa aatcctccct gcgagaaatt cattgtctgc gcggtacacc ccgtcgttgg
    27781 ggattgtttg gcgaaaacga tcaggaacag atcaacaata tttcactcgg tattatcgac
    27841 ctggtctatc ccgcacactg gcagccatac attgctcagg atctcggtca acaaaccaat
    27901 attgatatct atctcgaccg ccacgtagtg cgtcaggggc gatatctttc gctgcatgat
    27961 gaagtaaaaa actttccgtt acagcactgg ctgcgcagta cgattatcgc tgcgggctcg
    28021 ctgctggtgc tgtttatgct gttattctgg atcccgctgg atatgccgct gaaattcact
    28081 ctctcatgga tgaaaggcgc gcagaccatt gaagccacca gcgtaaaaca actggctgac
    28141 gctggcgtgc gggtaggcga tacattgcgt attagcggta cgggaatgtg taatattcgc
    28201 acttccggaa cctggagcgc gaaaaccaat tcaccttttt taccgtttga ctgctcgcag
    28261 atcatctgga atgacgcccg ctcattacca ttaccagaat ctgaactggt caacaaagcg
    28321 acggcattga ctgaagcagt taatcgccag ctgcacccta aaccggaaga tgaatctcgc
    28381 gtcagtgcct cattacgttc agcgattcaa aaatccggca tggtattgct tgatgatttt
    28441 ggcgacattg tactgaagac agcggattta tgttctgcca aagatgactg tgtgcgactg
    28501 aaaaatgcgc tggtcaatct cggcaacagt aaagactggg acgcgctggt aaaacgcgcc
    28561 aacgccggga agctcgatgg cgtgaatgtg ttattacgcc cggtgagtgc ggaatcgctg
    28621 gataacctgg tggcaacctc caccgcgccg tttatcacgc atgaaacggc gcgagcggca
    28681 caatcactaa acagtccggc ccccggcgga ttcctgattg tcagtgacga aggcagcgat
    28741 tttgttgatc agccctggcc ttcggcatca ctttacgact acccgccgca agaacagtgg
    28801 aacgctttcc agaaactggc acaaatgctg atgcatacgc cgtttaacgc cgaaggtatc
    28861 gtcacaaaaa tcttcactga cgccaatggt acgcagcata ttggccttca tccgatcccg
    28921 gatcgttccg gcctgtggcg ctatctcagc accacattgc tgctactgac gatgctgggt
    28981 agcgccattt acaatggcgt acaggcctgg cgtcgttacc agcgtcatcg cactcgcatg
    29041 atggagattc aggcctatta tgaaagctgc ctgaacccgc aactgatcac cccttcagaa
    29101 agccttatcg aataacacgt ttgcgcggca ggttatgcta ccctgtcgcg caaattgctt
    29161 cactctggag atttccctca tgcatatcaa cattgcctgg caggacgtag ataccgttct
    29221 gctggatatg gacggcacat tgctcgacct cgccttcgat aactatttct ggcaaaagct
    29281 ggtgcctgaa acatggggcg cgaaaaacgg ggttacgcca caggaagcga tggaatatat
    29341 gcgccagcaa tatcacgacg tacagcatac gctaaactgg tactgtcttg attactggag
    29401 tgagcaactg ggtctggata tctgtgcgat gaccaccgag atgggaccgc gtgccgtact
    29461 gcgtgaagat accattccgt ttcttgaggc actgaaagcc agcggtaagc agcgaatttt
    29521 gctcaccaat gcgcatccgc acaacctggc ggtaaaactt gagcataccg gtctggacgc
    29581 acaccttgat ttattacttt ccacccacac atttggttat ccgaaagagg atcagcggtt
    29641 atggcatgcg gtggccgaag ctacgggtct gaaagctgaa agaacgctgt ttattgatga
    29701 cagcgaagcg attctcgatg ctgccgcgca atttggtatt cgttactgcc tcggcgtgac
    29761 taatcctgat tccgggattg ccgagaaaca gtatcaacgc catccgtcac tgaatgacta
    29821 ccgccgcctg atcccctcgc taatgtgaag gagacgccat gaaagaaaaa cctgctgttg
    29881 aggttcgact ggataaatgg ctatgggctg cccgttttta taaaacccgc gcgctggccc
    29941 gtgaaatgat tgaaggcggt aaggtgcatt acaacgggca gcgcagcaag ccgagcaaaa
    30001 tcgtcgagct gaatgccacg ctcactctgc gccagggaaa tgacgaacgc acggtgattg
    30061 taaaggcgat tactgaacag cgtcgccccg ccagcgaggc agccttgctg tatgaagaga
    30121 ctgcggaaag tgtagagaaa cgcgaaaaaa tggcgatggc acgtaaactt aatgccttaa
    30181 ccatgccgca cccggaccga cgcccggaca aaaaagagcg ccgcgacctg ttacgattta
    30241 aacacggcga cagtgaataa ctgtcacctg caagagagat gattatgccg caacatgacc
    30301 aattacatcg ctatctgttt gaaaattttg ccgttcgcgg cgaactggta accgtttcgg
    30361 aaaccctgca acagatcctt gagaaccacg attatccgca gcccgttaaa aacgtgctgg
    30421 cagaactgct ggttgcgacc agcctgttaa ccgctacgct gaagtttgat ggtgatatca
    30481 ccgtacagct gcaaggcgac ggtccgatga atctggcggt tattaacggt aacaataacc
    30541 agcaaatgcg cggtgtggcg cgcgtgcagg gcgaaattcc agaaaatgcc gacctgaaaa
    30601 cgctggtcgg caatggttac gtggtgatca ccattacccc gagcgaaggc gaacgctatc
    30661 agggcgtggt tggtctggaa ggtgataccc tggcggcctg cctggaagat tactttatgc
    30721 gttctgaaca gctgccgacg cgcctgttta ttcgcaccgg cgacgtagac ggcaaaccgg
    30781 ctgcaggcgg tatgttgttg caggtaatgc ctgcgcaaaa tgcccagcag gacgactttg
    30841 accacctggc gacgctaacc gaaaccatca aaaccgaaga actgctgacc ttaccggcaa
    30901 acgaagtgtt gtggcgtttg tatcacgaag aagaggtgac ggtttacgat ccgcaggatg
    30961 tggagttcaa atgcacctgc tcgcgtgaac gttgcgccga tgcgctgaaa acgctgcctg
    31021 atgaagaagt tgatagcatc ctggcggaag atggcgaaat tgacatgcat tgtgattact
    31081 gcggtaacca ctatctgttc aatgcgatgg atattgctga aatccgcaac aacgcgtctc
    31141 cggcagatcc gcaagttcat taatgagtat gtccggcaga gagggctctg ccggatttta
    31201 cgacgtaatg gcctgatgtg ctgcgcttac caggcctgtg aaataaatcg caaattattg
    31261 ttggtcagat acagcgtcgt atccgttaaa agagttgtca cttacttaag tttatttcca
    31321 gcgtttcacc atctaccgtc agatcgcgcc acgctttcgg cctcgtcccc agttttccat
    31381 ccttcgcagc tgtgagtaat ttttctttcg ttatttcgtt cggaagtaaa ctcatatcaa
    31441 gcgcgtccca ttcttttctg tctgagtcaa agccatagac aatgactctg tcatcattaa
    31501 aagcaaacag gatccgctcc gcctgaccat cactattcag atcttgctcg accaggacgc
    31561 aggcgtcttt ttcaatacag gtcatcacgt tatagcgatc ctggattaag gccgaccaga
    31621 atgtcgcatc cggtttaaca gaaccagggg caattaacac attttcggct aatacttttt
    31681 ccgatacctg ttcctgaaga tgttgctctc catctaacgc catcagcaga tcccgcgcgc
    31741 gtttcgggtc tttcataaac ccggcatcgc ttttcagcga ctcaagcgcc gcacgtccat
    31801 agcgaccgct ctgttcgagc atgtagatag ttacctggtc tggcgtgttt ttgccgctct
    31861 gataacgcgc catatgactg ttcacgctaa tacgcatact gtccagcacc ggcgaattaa
    31921 gaagcaccag tatcaccaac accaataacg aaaccgcaag gttcactttg ccctgaagaa
    31981 caaggggatt ttgcccatta cgccagacga tactgacaaa ataccccagc gaccacacca
    32041 gtaacaccag caccgccagc gcgccctgca agcggtcgac agtccagccg tactgagcga
    32101 cccgcagcca taacgcccag gcggcgacga acacatacag cggagcaacc agcaaagcgg
    32161 ttttaatcag gcaacgcaac ggccccgtcc agggaagtga cgctttttgc ggatcgcgga
    32221 caatagccat taagatcaat tgcaaaaagg ccagcgttaa cagcaacccg gcggcggaga
    32281 tgtggcgaga aatcgcgctc aggcccgtaa acggcagagt gatgataaac atcagggtta
    32341 gcaatgatac taacggcagc aaccccgtgg cgattaacgt gaacaacttt tgaatagagt
    32401 cgattaaacg tgactgtgtg cgcgcgagaa tcaccgccag tgcggtaacc agacctaatg
    32461 taagatacat aaaccagtcg gttgcaaaaa agagcgtatt gaaaaacttg atgccaacaa
    32521 gtttaaacaa ctcaccccac agtaaaagca ccagccacgt caggccattg gcgagaaaaa
    32581 tcaccagtaa tataagtacg ttatgccata ctgactggta aaagtagcta tagcggctac
    32641 tgccattgcg gatacgaagg ctttgttgta tccacggtag caacaacatt gccatcaaca
    32701 gcagatagca gccaaaatcc caaatcgcct tttcagctct ccaggggttc atgccatcag
    32761 tttgccactt cagccaaccg ctcatccctg tcgtggcaat aaacaccagc gccagccatc
    32821 cccaaaggcg cttctgttta aaagagatca cggagaagag taaaacggat gaaaacgcaa
    32881 cggtcgcagg cacgccataa acaatccaac tgtgattttt tccggcaagc caggcgatca
    32941 acaggtagca aaccagcccc tgaagtaatc cggtagcaat catcccccag cgtgttgccg
    33001 gtgaaagttc aacgttatcc atgttggtta tccagaatca aaaggtgggt taattatcgc
    33061 atccgggcag tagtattttg cttttttcag aaaataatca aaaaaagtta gcgtggtgaa
    33121 tcgatacttt accgattgaa tttgcatcaa tttcattaag gaatgcgatt ccactcacaa
    33181 tattcccgcc atataaacca agatttaacc ttttgagaac attttccaca cctaaaatgc
    33241 tatttctgcg ataatagcaa ccgtttcgtg acaggaatca cggagttttt tgtcaaatat
    33301 gaatttctcc agatacgtaa atctatgagc cttgtcgcgg ttaacacccc caaaaagact
    33361 ttactattca ggcaatacat attggctaag gagcagtgaa atgcgcgtta acaatggttt
    33421 gaccccgcaa gaactcgagg cttatggtat cagtgacgta catgatatcg tttacaaccc
    33481 aagctacgac ctgctgtatc aggaagagct cgatccgagc ctgacaggtt atgagcgcgg
    33541 ggtgttaact aatctgggtg ccgttgccgt cgataccggg atcttcaccg gtcgttcacc
    33601 aaaagataag tatatcgtcc gtgacgatac cactcgcgat actttctggt gggcagacaa
    33661 aggcaaaggt aagaacgaca acaaacctct ctctccggaa acctggcagc atctgaaagg
    33721 cctggtgacc aggcagcttt ccggcaaacg tctgttcgtt gtcgacgctt tctgtggtgc
    33781 gaacccggat actcgtcttt ccgtccgttt catcaccgaa gtggcctggc aggcgcattt
    33841 tgtcaaaaac atgtttattc gcccgagcga tgaagagctg gcaggtttca aaccagactt
    33901 tatcgttatg aacggcgcga agtgcactaa cccgcagtgg aaagaacagg gtctgaactc
    33961 cgaaaacttc gtggcgttta acctgaccga gcgcatgcag ctgattggcg gcacctggta
    34021 cggcggcgaa atgaagaaag ggatgttctc gatgatgaac tacctgctgc cgctgaaagg
    34081 tatcgcttct atgcactgct ccgccaacgt tggtgagaaa ggcgatgttg cggtgttctt
    34141 cggcctttcc ggcaccggta aaaccaccct ttccaccgac ccgaaacgtc gcctgattgg
    34201 tgatgacgag cacggctggg acgatgacgg cgtgtttaac ttcgaaggcg gctgctacgc
    34261 aaaaactatc aagctgtcga aagaagcgga acctgaaatc tacaacgcta tccgtcgcga
    34321 tgcgctgctg gaaaacgtca ccgtgcgtga agatggcact atcgactttg atgatggttc
    34381 aaaaaccgag aacacccgcg tttcttatcc gatctatcac atcgataaca ttgttaagcc
    34441 ggtttccaaa gcgggccacg cgactaaggt tatcttcctg actgctgatg ctttcggcgt
    34501 gttgccgccg gtttctcgcc tgactgccga tcaaacccag tatcacttcc tctctggctt
    34561 caccgccaaa ctggccggta ctgagcgtgg catcaccgaa ccgacgccaa ccttctccgc
    34621 ttgcttcggc gcggcgttcc tgtcgctgca cccgactcag tacgcagaag tgctggtgaa
    34681 acgtatgcag gcggcgggcg cgcaggctta tctggttaac actggctgga acggcactgg
    34741 caaacgtatc tcgattaaag atacccgcgc cattatcgac gccatcctca acggttcgct
    34801 ggataatgca gaaaccttca ctctgccgat gtttaacctg gcgatcccaa ccgaactgcc
    34861 gggcgtagac acgaagattc tcgatccgcg taacacctac gcttctccgg aacagtggca
    34921 ggaaaaagcc gaaaccctgg cgaaactgtt tatcgacaac ttcgataaat acaccgacac
    34981 ccctgcgggt gccgcgctgg tagcggctgg tccgaaactg taatgatttg aagctggaga
    35041 atatctatcc agtatcttat agaaagcaaa acgggaggca ccttcgcctc ccgtttattt
    35101 acccttcttt tgtcgtgccc tgcgcccgcg ttaccggcac tggcagccag gcgcgaatgg
    35161 aaagcccgcc ccgctcgctg gtgccaagct ccagcatccc gttatggtta tccacgatac
    35221 gctgcacaat tgccagccct aatcccgtgc cgctaatggt gcgcgcactg tcgccgcgga
    35281 caaacggctg gaacaggtgc ttacgttgtt ccggcgcaat tcccggaccg tcatcttcca
    35341 cctggaacca ggcgcgattc ggctccgttc cgctgctgac tttgatccag ccattgccat
    35401 aacgggcggc gttgaccacc atattcgcca ccgcgcgttt gatcgacagc gggtgcattt
    35461 tcacttcaat gctgccgggg taaagcgcgg tttcaatttc ccgctcatag ccactttcgg
    35521 cagcaatcac ctcaccgagt actgcattaa gatccgccat ttccatcggc atctcctgcc
    35581 cggtgcgcag gtagtcgata aactgctcaa tgatggcgtt gcactcttcg atatctttat
    35641 tgatcgattc tgccagatag ccatcctgct cgctcatcat ctcagtcgcc aggcgaatac
    35701 gcgtcagcgg cgtgcgcaag tcgtgactta cccccgccat cagcagcgtg cggtcatccg
    35761 ccagttgctt aacaccagcc gccatatggt taaaggcacg ggtaacggaa cgcacctccg
    35821 aagcgccata ttcacgcagc ggcggcggaa taatcccttt accaacctgc aaggctgcgt
    35881 gttcgagatc gaccaacggt cggttctgga tacgaataaa cagccacgcc ccgcctatcg
    35941 ccaatagcat aatcgccagc gtatagcgga acagcggaga gaaatcgccc tgatgaattt
    36001 cggtcagcgg cacgcgtacc cagatattgg gcgacagcca ggttttcagc cagacgacag
    36061 gcgaactttt gttgacctca acgcgcactt ccgtcgggcc gcccagttgc tgtgccatct
    36121 gatggcttaa gaattcatag tgttgcgccc aacgcagacc tgcctcttcg gcagcctcgt
    36181 tggagtagag agagatcccc agctcacggt agatctcccg acggaaagcg ggaggcacaa
    36241 ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat caacatacgc acttcgtacg
    36301 cgaggacttt attaaactgc tggaggctcg gcaaaatcgc gaagttcagc accaccagat
    36361 aagtcgtcac caggctggcg aacagcaagg tgacgatgag caataacgta cgggcaaatg
    36421 aacttcgtgg cgagaagcgc aatcgcctca tgctttagag ccgtccggta caaagacgta
    36481 gcccaggccc caaacggtct gaatgtaacg cggatgcgct ggatcttctt ctaccatgcg
    36541 gcgcagacgc gaaatctgca cgtcgatgga gcgttccatt gcggaatatt cacgaccacg
    36601 ggcaaggttc atcagcttat cgcgggagag cggctcacgc ggatggctga ccagtgcctt
    36661 cagtaccgca aactcaccgc tggtgagcgg catcggctca tcttcgcgga acatttcgcg
    36721 cgtaccgagg ttaagtttga acttaccgaa agcaattacc gcctcttcct gtgacggtgc
    36781 gcctggcagt tcgttcgcct gacgacgcag caccgcacga atacgggcca gcagttcacg
    36841 cgggttaaac ggttttggaa tgtagtcgtc agcgccaatc tccaggccta cgatacggtc
    36901 cacttcttcc cctttcgccg tcaccataat gatcggcatc gggttgctct gactacgaag
    36961 acgtcggcaa atcgacaagc catcttcacc aggtaacatt aaatccagta ccataagatg
    37021 gaaagattca cgagtcagca ggcgatccat ctgttctgca ttagcgacgc ttcgaacctg
    37081 gaagccttgt tcggtgagat aacgttccag cagcgcacgc aggcgcatgt cgtcatcgac
    37141 caccagaatc ttgtagttct cttgcattgt ttgtactccc aaaggttcgc aacaatttgt
    37201 aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct aaatctggta tgaatttcag
    37261 cctaaattgt tacaaagcat attaaacagc agcttaagta tacaatttat tcggcgaaac
    37321 attattgatt ctgttgatat gatcacgtta tacccaatgt gcgcattatc aaacagacaa
    37381 agggaatcaa cgagatgaaa acgcccctgg ttacccggga agggtatgaa aaactcaaac
    37441 aagagcttaa ttatctctgg cgtgaagaac gcccggaggt cacaaaaaag gtgacctggg
    37501 ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca gtataataaa aagcgtctgc
    37561 gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat ggaaaatctc aaaatcgtcg
    37621 attactcccc tcagcaggaa ggcaaagtct tttttggcgc gtgggtggag attgaaaacg
    37681 acgatggcgt gactcaccgt ttccgtattg tcggctacga tgaaattttt ggccgtaaag
    37741 attacatctc tatcgattcc ccgatggccc gcgcattgct gaaaaaagaa gtcggcgatc
    37801 tggcggtggt gaatacccct gccggggaag cgagctggta tgttaatgct atcgagtacg
    37861 tgaaaccgta aggaagagtc ttaacctcct gccgatggct ggcatttttg ccagccagtc
    37921 cgtataacta tcccctgatt tttgatccga aaagatgaac tcaaaccatg atgaatgatt
    37981 cgttctgccg cattattgcg ggtgaaattc aggcgcgccc ggaacaggtt gacgctgccg
    38041 ttcgcctgct tgacgaaggg aataccgtgc cgtttatcgc acgttatcgt aaggaaatca
    38101 ccggcggtct ggatgacacg cagctgcgta atctggaaac gcgtctgagc tatctgcgcg
    38161 agctggaaga gagacgtcag gcgatcctca agtccatttc cgagcaaggc aaactcaccg
    38221 atgatctggc gaaggccatc aacgccaccc taagcaaaac cgaactcgaa gacctctacc
    38281 tgccctacaa acctaaacgc cgcacccgcg ggcaaatcgc cattgaagca gggcttgagc
    38341 cgttggctga cctgctgtgg agcgatccgt cacacacgcc agaagtcgcc gctgcacaat
    38401 atgttgatgc cgataaaggc gtggcagata ccaaagccgc gctggatggc gcgcgctata
    38461 tcctgatgga acggtttgcc gaagatgccg cgctgctggc gaaagtgcgt gattatctgt
    38521 ggaagaacgc gcatttggtt tctacggtgg tgagcggtaa agaagaggaa ggggcgaaat
    38581 tccgcgacta tttcgatcat cacgaaccgt tgtccacggt gccttctcac cgcgcgctgg
    38641 cgatgttccg tgggcgtaac gaaggcgtac tccagctttc gctgaatgcc gatccgcagt
    38701 tcgatgagcc gcccaaagag agctattgcg agcaaatcat catggatcac cttggcctgc
    38761 gcctgaacaa tgccccggcg gatagctggc gcaaaggcgt agtgagctgg acctggcgca
    38821 tcaaggtgct gatgcatctg gaaaccgaac tgatgggtac cgtgcgcgaa cgcgcggaag
    38881 atgaagcaat caacgtcttt gcccgtaacc tgcacgatct gctgatggcg gcccctgccg
    38941 gactgcgtgc aacgatgggc ctcgatccgg gtctgcgtac tggggtaaaa gtggcggtgg
    39001 tcgatgccac tggcaaactg gtggcgaccg ataccattta cccgcacacc ggacaagccg
    39061 caaaagcagc gatgaccgtt gctgccttgt gtgaaaaaca taacgttgaa ctggtggcga
    39121 tcggcaacgg tacagcttcc cgcgaaactg aacgtttcta tctcgacgtg cagaagcagt
    39181 tcccgaaagt gaccgcacag aaagtgatcg tcagcgaagc tggcgcgtcg gtttactcag
    39241 cttccgagct ggcagcgcag gagttcccgg atctcgacgt ttcgctgcgt ggtgcggtat
    39301 ctatcgcccg ccgtttgcag gatccgctgg cggagctggt gaaaatcgat ccgaaatcta
    39361 tcggcgtagg tcagtatcag catgacgtca gccagacgca actggcccgc aaactggacg
    39421 cagtagtaga agactgcgta aacgccgttg gcgtcgatct caacactgct tctgttccgc
    39481 tgttaacccg cgtggcgggc ctgacgcgca tgatggcgca aaacatcgtg gcctggcgcg
    39541 atgagaacgg tcagttccag aaccgtcagc aactgctgaa agtgagccgt ctggggccga
    39601 aagccttcga gcagtgcgcg ggcttcttgc gcattaacca tggtgataac ccgctggacg
    39661 cgtctaccgt ccacccggaa gcctatccgg tggtggaacg cattctggca gcaacacagc
    39721 aggcactgaa agatctgatg ggtaacagca gcgaactgcg taacctgaaa gcgtctgact
    39781 ttactgatga aaaattcggt gtgccgacag taactgacat catcaaagag ctggaaaaac
    39841 cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt tgccgatggc gtcgagacaa
    39901 tgaacgacct gcaaccgggt atgatcctcg aaggtgcggt gaccaacgtc accaactttg
    39961 gcgcgtttgt cgatatcggc gtgcatcagg acggcctggt tcacatctct tcattgtcga
    40021 acaagtttgt agaagatccg cataccgtgg tgaaagcggg cgacattgtg aaggtgaaag
    40081 tgctggaagt ggatcttcag cgtaaacgta tcgccctgac catgcgtctg gacgagcagc
    40141 ctggcgaaac caacgcccgt cgcggcggcg gtaatgaacg cccgcaaaac aaccgcccgg
    40201 cagccaaacc gcgcggacgt gaagcgcagc ctgccggtaa cagcgcgatg atggacgcgc
    40261 tggcggcagc aatgggcaaa aaacgttaaa cgccctgatt gacccctaaa cctgcgcgat
    40321 aaggtgtcca cgccgcatct ggcaacgaac gccttgtctg acataaaagt gccggagaat
    40381 atctccggca tttttattcc acagccaaac tcacaatata ttccggcaac atttatcact
    40441 tcattaacaa ctgaaacctt aattaaacat tagccagtcc tggtaattcg ctattcgaat
    40501 tatattttcg ctgcgatata accttgagcc acatcaacat tgagtcagat tattattcaa
    40561 accaacattc gcacacattt taagtattgc tgatagaaac cattctcatt atcattgtgc
    40621 tgttgattat ttaatctctc cttcgttggc aaatcatctg gtctcatgtc gctgtcaaac
    40681 gccccatgag gtagttatcc agttaatgag aaacaagtag gcacctatgc aatacactcc
    40741 agatactgcg tggaaaatca ctggcttttc ccatgaaatc agcccggcat atcgccaaaa
    40801 actgctttct cttggcatgt tacctggctc ctcttttaat gtggtgcgcg tcgctccact
    40861 cggcgacccc attcatatcg aaacccgtcg tgtgagcctg gtattacgca aaaaagatct
    40921 ggccttatta gaagtggaag cggtttcctg ttaatacggt gataacaaca atgaaaaaat
    40981 taaccatagg cttaattggt aatccaaatt ctggcaagac aaccttattt aaccagctca
    41041 ctggcgcacg tcagcgtgta ggtaactggg ctggcgttac cgtcgaacgt aaagaagggc
    41101 aattctccac caccgatcat caggtcacgc tggtggacct gcccggcacc tattctctga
    41161 ccaccatctc atcgcagacc tcgctcgatg agcaaatcgc ctgtcactac attttgagtg
    41221 gcgacgccga cctgctgatt aacgtggtgg atgcgtctaa ccttgagcgt aacctgtacc
    41281 tgacgctaca actgctggaa ctcggcattc cctgcatcgt ggcactgaac atgctcgaca
    41341 ttgccgagaa gcaaaatatt cgtattgaaa ttgatgctct gtcggcgcgt cttggctgcc
    41401 cagtgatccc gctggtttca acccgtggtc gcggtattga agcgctcaag ctggcgattg
    41461 atcgctataa agctaacgag aatgtggaac tggtgcatta cgcacagccg ctactcaacg
    41521 aagcagattc actggcaaaa gtgatgcctt ccgacatccc gctgaaacaa cgtcgctggc
    41581 tgggcctgca aatgctggaa ggcgatatct acagccgcgc ctacgccggt gaagcgtcgc
    41641 agcatctgga tgccgccctc gcccgtctgc gtaatgagat ggacgatccg gcgctgcaca
    41701 ttgccgatgc gcgttaccag tgcattgctg ccatctgtga tgtggtaagc aacaccctga
    41761 cggcagaacc cagccgtttc accactgcgg tagataaaat cgtgctcaac cgtttcctcg
    41821 gtctgccgat tttcctcttt gtgatgtacc tgatgttcct gctggctatc aacatcggcg
    41881 gggcgttaca gccgctgttt gacgtcggct ccgtggcgct atttgtgcat ggtattcaat
    41941 ggattggcta cacgctccac ttcccggact ggctgactat cttcctcgcc cagggcctgg
    42001 gtggcggcat taacaccgtg ctgccactgg tgccgcagat tggcatgatg tacctgttcc
    42061 tctccttcct tgaggactcc gggtatatgg cgcgtgcggc gtttgtgatg gaccgtctga
    42121 tgcaggcgct gggcttgccg gggaaatcct ttgtgccgct gatcgtcggt ttcggttgta
    42181 acgtaccgtc ggtaatgggt gcacgtacgc ttgatgcacc gcgtgaacgt ctgatgacca
    42241 tcatgatggc accgtttatg tcctgcggcg cgcgtctggc tatcttcgca gtattcgcgg
    42301 ctgccttctt cgggcagaac ggtgcgctgg cggtcttctc gctgtatatg ctgggtattg
    42361 tgatggcggt gctgactggc ctgatgctca agtacaccat catgcgcggt gaagcgacgc
    42421 cgtttgtcat ggagttgccg gtctatcatg taccacacgt taaaagcctg attatccaga
    42481 cctggcagcg tctgaaaggc ttcgttctgc gtgctggtaa agtgatcatc atcgtcagca
    42541 ttttcctgag cgctttcaac agcttctcgc tgagcgggaa aatcgtcgat aacatcaacg
    42601 actcggcgct ggcgtccgtc agccgggtga tcaccccggt cttcaagcca attggcgtgc
    42661 atgaagataa ctggcaggca acggttggcc tgtttacagg tgccatggcg aaagaagtgg
    42721 tagtgggtac gctcaacacc ctctacaccg cagaaaatat tcaggacgaa gagttcaatc
    42781 cggcagaatt taacctcggt gaagagctgt tcagtgcgat agatgaaacc tggcagagcc
    42841 tgaaagacac cttcagcctt agcgtactga tgaaccccat tgaagccagc aaaggcgacg
    42901 gcgaaatggg taccggggcg atgggcgtga tggatcagaa attcggtagc gcagcagcag
    42961 cttacagcta cctgattttc gtcctgctgt atgtaccatg tatctcggtg atgggagcca
    43021 tcgcccgtga atcaagccgt ggctggatgg gcttctccat cctgtggggg ctgaatatcg
    43081 cttactcgct ggcaacattg ttctatcagg tcgccagcta cagtcagcac ccaacttaca
    43141 gcctggtgtg cattctggcg gttatcctgt ttaacatcgt ggttatcggt ctgctgcgcc
    43201 gcgcgcgtag ccgggtggat atcgaactgc tggcaacccg caagtcggta agcagttgct
    43261 gcgcagccag caccaccggt gattgccatt aatggcttca cttattcagg tgcgcgattt
    43321 gctggcgtta cggggccgta tggaagcggc ccagataagc cagacattga acactccaca
    43381 gccaatgatt aacgccatgc tgcaacaact ggaaagtatg ggcaaagccg tgcggattca
    43441 ggaagaacct gacggctgcc tctctggcag ttgtaaaagc tgcccggaag gaaaagcctg
    43501 tctgcgcgag tggtgggcgc tgcgttaacc ttactccatc gcctgttttg gaaagcccgg
    43561 tatgcgtctg catccgggct tttttgcgtg cggctttcca taaaaatgca actcttgcag
    43621 cacggcgtaa gttcctttga aagcatctcg cagggatgaa aactcgctaa tgcacagatg
    43681 tggagtggcg cgtagagtcg cggcattcaa acaacaggtg aaggaacgcc atgagtaaaa
    43741 agcagagttc caccccacat gatgcgctgt tcaaactctt tttacgccaa ccggacacgg
    43801 ctcgcgattt tcttgcgttc catttaccgg cccccattca cgcgctctgt gatatgaaaa
    43861 ccctcaagct ggagtcgagc agctttattg atgacgatct gcgtgaaagc tattccgatg
    43921 tgctgtggtc ggtgaaaacg gaacaaggac caggatacat ctattgtctg attgaacatc
    43981 aaagcacctc aaacaaactg atcgcatttc gcatgatgcg ttacgctatt gccgcaatgc
    44041 aaaatcacct tgatgctgga tacaaaacgt tgccgatggt ggtgctattg ttgttttacc
    44101 acggtattga aagcccctat ccctattcgc tgtgttggct ggattgtttc gccgatccca
    44161 aactggcaag gcagctttat gcctccgcat ttccgctgat tgatgtcacc gtcatgcctg
    44221 atgatgaaat catgcagcac cgacgtatgg cgctgctgga gttaattcaa aaacatattc
    44281 gtcaacgcga cctgatgggg ctggtagagc aaatggcctg cttattaagt agtggatacg
    44341 ctaatgacag acaaatcaaa gggctgttta attacatact gcaaaccggc gacgcggtac
    44401 gttttaacga ttttatcgac ggcgttgccg aacgttcacc gaaacacaag gagagtttaa
    44461 tgacaattgc ggaaagattg cggcaggagg gggaacaatc caaagccctg catatagcca
    44521 aaataatgct tgaatccgga gtccctctta cagacatcat gcgctttacc ggggtgtcag
    44581 aagaagagtt ggctgcggcg agccagtaaa tttctgtctc gccatttcaa aagttgccta
    44641 caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg aaataaacgg
    44701 cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt tatccagcat
    44761 cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca aaaacggcat
    44821 gggcacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca gcccgccatt
    44881 aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg cccgcgcatc
    44941 ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca ctgtacgctg
    45001 aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta tccccggcca
    45061 ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct gaacacgctc
    45121 gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc ctaaccaaat
    45181 ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg acagcgcacc
    45241 aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa aatgcgagct
    45301 aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt gcagcagcac
    45361 aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc gctattgttc
    45421 tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct ggctatgccg
    45481 aatgccactg gcgttaggtc attgggggat ttgttcggtc tgctcacgcg ctgcccgtac
    45541 agataaaacg ttatgtccac aatgtggatt acccgccaca cactcacatc ttccctgcgg
    45601 tcgctgcctg caaaaaccac cgccctggca aagactggtc acggttgccg actatgcgcc
    45661 gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa tcgccagcgc
    45721 cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg ggttgcaatt
    45781 gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc ggggatttaa
    45841 tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat gggatagcga
    45901 agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc ggctgcgcaa
    45961 gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc atatggtgat
    46021 tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt tgcttttacg
    46081 caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga gcctcgatga
    46141 tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg atccgtattt
    46201 ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa gggacacaaa
    46261 tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt tcttattgtc
    46321 cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg accgcgtatg
    46381 ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt accgaccagt
    46441 tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg gcagacgatg
    46501 caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca cagcttgctg
    46561 gtcacggtgg tcgcgtttcg ctgatggaaa tcacagaaga cggttacgcc attctgcaat
    46621 ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa gggatcgaga
    46681 agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc gaacaccagc
    46741 gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg atgcgacgct
    46801 aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc attgctttgg
    46861 caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat ttaaatttac
    46921 cccgctctgg tgattctcaa acgccagatg ttacccgtat cattcacatg ggtaccaaac
    46981 atactcctga catctgacta caataattag ttttagtggg tatcagtcgt ggtgccgcaa
    47041 tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac gttacccata
    47101 acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt gtaatcttgt
    47161 tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc ctcgtggcgc
    47221 ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc atcaaagccg
    47281 gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca atgctgggca
    47341 aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt gccaaatttg
    47401 gtaaaaaaca catccagtgg gcggtggtac tgaccggttt taccgttggt tttgccctgt
    47461 tctatgaagt gggctttgtg ctgatgctgc cgctggtgtt taccatcgcg gcttctgcga
    47521 atattccact gttgtatgtt ggtgtaccaa tggcagctgc actgtctgtg acccacggct
    47581 tcctgccacc gcatccgggt ccgactgcga ttgccaccat tttcaatgcc gatatgggta
    47641 aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc ggtccggttt
    47701 acgctcgcgt gttgaaaggt atcgataagc caattccgga aggtctctac agcgcgaaaa
    47761 ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacttct ctggtgccgg
    47821 ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt cacgctttcc
    47881 tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc gtgctgattg
    47941 cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac gacacgctgg
    48001 tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc ggtgcgttca
    48061 agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg cacgaaacca
    48121 acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc gcgctgggtt
    48181 ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt gcaacgacgg
    48241 gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt ttctctcatg
    48301 tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc ggcgagacca
    48361 tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta ggctgtctgc
    48421 tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg gcatcaaccg
    48481 cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc agcaacttgt
    48541 tcacgccatc atcggcaaac atcgtctcaa gcgttgcgga aagcttgcgt cgccagtttt
    48601 tatactggta actggtgcca ggaatattca ccggttcggc catatccagc cagtcttccg
    48661 gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa ccacggttca
    48721 gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc agacaaccat
    48781 atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga tacagaccgc
    48841 gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga tccccgctct
    48901 cccagtaacc gcgtagcgta ggcaggtcat gtgtcgccgc aaccgccatc gactgctccg
    48961 gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag agcactttgt
    49021 aggagtacac accgctgctg cgcagcttac cgacaatctc taccggtacg gtaccgagat
    49081 cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc gagagcagat
    49141 catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac ggtatccacc
    49201 acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt tgcatattgg
    49261 cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc gggtccattg
    49321 gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg ccaaccgacg
    49381 ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc gctacgccaa
    49441 ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc tcccagcagg
    49501 cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg tcattacgat
    49561 gttcttcgca gaactgacgc acttctggtg aatccacgtt ctgatacatc tctggccatg
    49621 caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc gcatcaaagg
    49681 ctgcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac gcggtcatct
    49741 gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc atttttaggg
    49801 cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc gtctgttgcg
    49861 tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct tcaacggcgt
    49921 taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat gggctggcac
    49981 tctccggatt tgccggatag agcgcatgaa tcgggttcag gccgatgaac gacccgccac
    50041 gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata ccccagtttt
    50101 tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt ttattcagca
    50161 acgcctgcgg ttcgtaacag cgtttcgggg cgacaatcac ccggcaatgc gcgcgctggt
    50221 cgtcctgggt gagtgtcagc gtgtgataac cttccggcag cttcgtcggt agattgaacg
    50281 ctttgccccc cgttacatgg cctttgtact gtgttccttc ttcggtggtc agcagccagc
    50341 tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta taaaccatga
    50401 cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc gcgtcaagca
    50461 aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg atgtaattgg
    50521 ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc gagcttcctt
    50581 agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc gagctaaaca
    50641 taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg tcgcggtaca
    50701 gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc accagatacg
    50761 gatcgccgcc ttgtttgccg atactgtgca gcatctggtc gaaggcatgc ttatcgccgt
    50821 cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta tctttcttcc
    50881 gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc acggtatgac
    50941 caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc gcaccatcca
    51001 gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta ccggaagctt
    51061 ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt ttttccgccg
    51121 ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc agtggatcgt
    51181 tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc aggtagtagc
    51241 ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc tgcgggtttt
    51301 cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg cgtttgtact
    51361 catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca ataccggtac
    51421 gaactttcac aaactccgcc agacggactt tattcgcctg cttgatctcg cgatattgct
    51481 gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg agatcgttag
    51541 cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg cactgtttga
    51601 tccagcgacg tggggtaata ccgttggtga cgttatggaa tttgttcggc catagctggt
    51661 gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca acaccgttca
    51721 ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg tgcaccaccg
    51781 ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc gttttaaagc
    51841 gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc accagtttca
    51901 catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg aaagttttgc
    51961 tggtaatggc ccaggcgtca tcccagctca tctggtgctc atcgatcagc acgcgcagca
    52021 gttctggaat cgcgatagtt gggtgggtat cgttcagctg aataacttcg taatccgcca
    52081 gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct accgaacagg
    52141 cacactggaa gtactgctgc atcaagcgca gctttttacc ggcagtatgg ttgtcgtttg
    52201 gatagagaac tttggtcagt ttttccgcat tgatgccctg ctgttcggca cgcaggaaat
    52261 caccgtcgtt aaatttagtc agatcaaacg gatgcgcgtg cgtcgcctgc cacagacgca
    52321 gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct tgaccggtaa
    52381 tggtaaactc cggctcccag cgtccgtctt tcgtcacttt accgccaatc cctacctgca
    52441 catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag tcatccggcg
    52501 cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga tagttcagac
    52561 cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac gccgccagac
    52621 gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg tccgtcagat
    52681 tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga ttcaacaggt
    52741 tgttgcccgt caggcgacca atcaaaaact ccattgagat gtagttaaca tgtcgctgat
    52801 tcgccaccgg cttggcgaat ggctgagcac gcagcatttc ggccagtgct tcactcactg
    52861 ctagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa cgctgccact
    52921 gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac ataggagttc
    52981 cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg atgttgacct
    53041 tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag gaaatgtgat
    53101 ctcaaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg caatacagcg
    53161 cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa agcggaaggt
    53221 aactctataa attaagtaaa ggagtgaaac
//
LOCUS       DAPHJE010000013        52367 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPHJE010000013 DAPHJE010000000
VERSION     DAPHJE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.013"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            196..348
                     /gene="mokC"
                     /locus_tag="RH222_000821"
     CDS             196..348
                     /gene="mokC"
                     /locus_tag="RH222_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV3795881.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(452..1582)
                     /gene="dnaJ"
                     /locus_tag="RH222_000822"
     CDS             complement(452..1582)
                     /gene="dnaJ"
                     /locus_tag="RH222_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV3795882.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1671..3587)
                     /gene="dnaK"
                     /locus_tag="RH222_000823"
     CDS             complement(1671..3587)
                     /gene="dnaK"
                     /locus_tag="RH222_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV3795883.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQARNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLIEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3964..4368
                     /locus_tag="RH222_000824"
     CDS             3964..4368
                     /locus_tag="RH222_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV3795884.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4394..5107
                     /gene="msyB"
                     /locus_tag="RH222_000825"
     CDS             4394..5107
                     /gene="msyB"
                     /locus_tag="RH222_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV3795885.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5256..5822
                     /gene="satP"
                     /locus_tag="RH222_000826"
     CDS             5256..5822
                     /gene="satP"
                     /locus_tag="RH222_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV3795886.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5857..6444)
                     /gene="mog"
                     /locus_tag="RH222_000827"
     CDS             complement(5857..6444)
                     /gene="mog"
                     /locus_tag="RH222_000827"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV3795887.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6559..7512)
                     /gene="tal"
                     /locus_tag="RH222_000828"
     CDS             complement(6559..7512)
                     /gene="tal"
                     /locus_tag="RH222_000828"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV3795888.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7791..9221
                     /locus_tag="RH222_000829"
     CDS             7791..9221
                     /locus_tag="RH222_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV3795889.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9291..10067
                     /gene="yaaA"
                     /locus_tag="RH222_000830"
     CDS             9291..10067
                     /gene="yaaA"
                     /locus_tag="RH222_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV3795890.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10220..10516)
                     /locus_tag="RH222_000831"
     CDS             complement(10220..10516)
                     /locus_tag="RH222_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV3795891.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10730..12016)
                     /gene="thrC"
                     /locus_tag="RH222_000832"
     CDS             complement(10730..12016)
                     /gene="thrC"
                     /locus_tag="RH222_000832"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV3795892.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12017..12949)
                     /gene="thrB"
                     /locus_tag="RH222_000833"
     CDS             complement(12017..12949)
                     /gene="thrB"
                     /locus_tag="RH222_000833"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV3795893.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12951..15413)
                     /gene="thrA"
                     /locus_tag="RH222_000834"
     CDS             complement(12951..15413)
                     /gene="thrA"
                     /locus_tag="RH222_000834"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV3795894.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLTAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15439..15558)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15494..15559)
                     /gene="thrL"
                     /locus_tag="RH222_000835"
     CDS             complement(15494..15559)
                     /gene="thrL"
                     /locus_tag="RH222_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV3795895.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15773..16459)
                     /locus_tag="RH222_000836"
     CDS             complement(15773..16459)
                     /locus_tag="RH222_000836"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV3795896.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16859..16999)
                     /locus_tag="RH222_000837"
     CDS             complement(16859..16999)
                     /locus_tag="RH222_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795897.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17095..17811
                     /gene="arcA"
                     /locus_tag="RH222_000838"
     CDS             17095..17811
                     /gene="arcA"
                     /locus_tag="RH222_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV3795898.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17870..19222)
                     /gene="creD"
                     /locus_tag="RH222_000839"
     CDS             complement(17870..19222)
                     /gene="creD"
                     /locus_tag="RH222_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV3795899.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19280..20704)
                     /gene="creC"
                     /locus_tag="RH222_000840"
     CDS             complement(19280..20704)
                     /gene="creC"
                     /locus_tag="RH222_000840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV3795900.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20704..21393)
                     /gene="creB"
                     /locus_tag="RH222_000841"
     CDS             complement(20704..21393)
                     /gene="creB"
                     /locus_tag="RH222_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV3795901.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21406..21879)
                     /gene="creA"
                     /locus_tag="RH222_000842"
     CDS             complement(21406..21879)
                     /gene="creA"
                     /locus_tag="RH222_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV3795902.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22090..22959
                     /gene="robA"
                     /locus_tag="RH222_000843"
     CDS             22090..22959
                     /gene="robA"
                     /locus_tag="RH222_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV3795903.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22956..23603)
                     /gene="gpmB"
                     /locus_tag="RH222_000844"
     CDS             complement(22956..23603)
                     /gene="gpmB"
                     /locus_tag="RH222_000844"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV3795904.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23655..24167
                     /gene="yjjX"
                     /locus_tag="RH222_000845"
     CDS             23655..24167
                     /gene="yjjX"
                     /locus_tag="RH222_000845"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV3795905.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(24314..24640)
                     /gene="trpR"
                     /locus_tag="RH222_000846"
     CDS             complement(24314..24640)
                     /gene="trpR"
                     /locus_tag="RH222_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV3795906.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24730..26667)
                     /gene="sltY"
                     /locus_tag="RH222_000847"
     CDS             complement(24730..26667)
                     /gene="sltY"
                     /locus_tag="RH222_000847"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV3795907.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNIGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26878..28545
                     /gene="ettA"
                     /locus_tag="RH222_000848"
     CDS             26878..28545
                     /gene="ettA"
                     /locus_tag="RH222_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV3795908.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28652..28819)
                     /locus_tag="RH222_000849"
     CDS             complement(28652..28819)
                     /locus_tag="RH222_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795909.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(28853..30085)
                     /gene="nadR"
                     /locus_tag="RH222_000850"
     CDS             complement(28853..30085)
                     /gene="nadR"
                     /locus_tag="RH222_000850"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV3795910.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30106..31488)
                     /gene="radA"
                     /locus_tag="RH222_000851"
     CDS             complement(30106..31488)
                     /gene="radA"
                     /locus_tag="RH222_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV3795911.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31537..32505)
                     /gene="serB"
                     /locus_tag="RH222_000852"
     CDS             complement(31537..32505)
                     /gene="serB"
                     /locus_tag="RH222_000852"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV3795912.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32611..33255
                     /locus_tag="RH222_000853"
     CDS             32611..33255
                     /locus_tag="RH222_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV3795913.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33283..34299
                     /gene="lplA"
                     /locus_tag="RH222_000854"
     CDS             33283..34299
                     /gene="lplA"
                     /locus_tag="RH222_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV3795914.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            34331..34594
                     /locus_tag="RH222_000855"
     CDS             34331..34594
                     /locus_tag="RH222_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3795915.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34755..35474)
                     /gene="deoD"
                     /locus_tag="RH222_000856"
     CDS             complement(34755..35474)
                     /gene="deoD"
                     /locus_tag="RH222_000856"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV3795916.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35554..36777)
                     /gene="deoB"
                     /locus_tag="RH222_000857"
     CDS             complement(35554..36777)
                     /gene="deoB"
                     /locus_tag="RH222_000857"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV3795917.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36829..38151)
                     /gene="deoA"
                     /locus_tag="RH222_000858"
     CDS             complement(36829..38151)
                     /gene="deoA"
                     /locus_tag="RH222_000858"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV3795918.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38278..39057)
                     /gene="deoC"
                     /locus_tag="RH222_000859"
     CDS             complement(38278..39057)
                     /gene="deoC"
                     /locus_tag="RH222_000859"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV3795919.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39315..40865
                     /locus_tag="RH222_000860"
     CDS             39315..40865
                     /locus_tag="RH222_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV3795920.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGCYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40837..41700
                     /locus_tag="RH222_000861"
     CDS             40837..41700
                     /locus_tag="RH222_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV3795921.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGVGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41734..42513)
                     /locus_tag="RH222_000862"
     CDS             complement(41734..42513)
                     /locus_tag="RH222_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3795922.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLVMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(42510..43583)
                     /locus_tag="RH222_000863"
     CDS             complement(42510..43583)
                     /locus_tag="RH222_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV3795923.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVVPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43705..43866)
                     /locus_tag="RH222_000864"
     CDS             complement(43705..43866)
                     /locus_tag="RH222_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV3795924.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(43993..44598)
                     /gene="osmY"
                     /locus_tag="RH222_000865"
     CDS             complement(43993..44598)
                     /gene="osmY"
                     /locus_tag="RH222_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV3795925.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(44991..46580)
                     /gene="prfC"
                     /locus_tag="RH222_000866"
     CDS             complement(44991..46580)
                     /gene="prfC"
                     /locus_tag="RH222_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV3795926.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKTGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46671..47348)
                     /gene="yjjG"
                     /locus_tag="RH222_000867"
     CDS             complement(46671..47348)
                     /gene="yjjG"
                     /locus_tag="RH222_000867"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV3795927.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47363..47809)
                     /gene="rimI"
                     /locus_tag="RH222_000868"
     CDS             complement(47363..47809)
                     /gene="rimI"
                     /locus_tag="RH222_000868"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV3795928.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47778..48191)
                     /gene="holD"
                     /locus_tag="RH222_000869"
     CDS             complement(47778..48191)
                     /gene="holD"
                     /locus_tag="RH222_000869"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV3795929.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48294..49325
                     /gene="rsmC"
                     /locus_tag="RH222_000870"
     CDS             48294..49325
                     /gene="rsmC"
                     /locus_tag="RH222_000870"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV3795930.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFSRHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            49626..49712
                     /locus_tag="RH222_000871"
     tRNA            49626..49712
                     /locus_tag="RH222_000871"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49660..49662,aa:Leu,seq:cag)
     gene            complement(49751..49987)
                     /locus_tag="RH222_000872"
     CDS             complement(49751..49987)
                     /locus_tag="RH222_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV3795931.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            50128..50916
                     /gene="fhuF"
                     /locus_tag="RH222_000873"
     CDS             50128..50916
                     /gene="fhuF"
                     /locus_tag="RH222_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV3795932.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(50954..51631)
                     /gene="bglJ"
                     /locus_tag="RH222_000874"
     CDS             complement(50954..51631)
                     /gene="bglJ"
                     /locus_tag="RH222_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV3795933.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(51589..52314)
                     /locus_tag="RH222_000875"
     CDS             complement(51589..52314)
                     /locus_tag="RH222_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV3795934.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELKREMSHS"
BASE COUNT        12344 a        14170 c        13160 g        12693 t
ORIGIN      
        1 aatcgataaa acagaatggg ttaaagagag cgatataata gcggcgggtg cttgaggctg
       61 tctgtctcag gcattagctg aacggcagat agagaaaagc cccgagtgat attttaccat
      121 caacccgagg ccccctatat gctgaacaca tgtagagtgc ctcttactga ccgtaaggtc
      181 aaggagaaga gagcaatgaa gcagcataag gcgatgattg tcgccctgat cgtcatctgt
      241 atcaccgccg tagtggcggc gctggtaacg agaaaagacc tctgtgaggt tcacatccga
      301 actggccaga cggaggttgc tgttttcacg gcttacgaat ccgagtaaga gcaacggcgg
      361 ggagtgatcc ccgccacttt acaggtgctc gcatatcttc aacgcaccct atttttaccc
      421 aggcctgccc acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct
      481 tcacaccatc aaagaagctc tttgagcgcg ggctgttgtg ctcgccggtt gggccaccga
      541 agctttcttg cagctcttgc agcagctgtt tctgcttctc gttcaggcct accggtgttt
      601 cgacgacaac gcggcacagt aaatcaccct gtgcgccacc gcggacagac ttgacgcctt
      661 taccgcgcat acggaacagc ttaccagtct gggtttcgcc aggcactttc agtttgacgc
      721 gaccatcaag ggtcggtact tcgatttcgc cacccagcgc cgccatagcg aagttgatcg
      781 ggacttcgca atacaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa
      841 cctgaacgta cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa
      901 gacggatgcg gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc
      961 gctcaacacg accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcgac
     1021 cctgacagtg tggacaggtc tgctgcacgg caaagaaacc ctgacgcatc tgcacctgac
     1081 cagaaccatg acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt
     1141 ggcaaacgtc acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag
     1201 cttcttcgag ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac
     1261 gaccacgtcc gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg
     1321 cgccgccgcc aaaaccgccg ccgcccatac caccttgctc aaacgcagca tgaccatact
     1381 gatcgtatgc cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt
     1441 taaatttcgc ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca
     1501 ggcgtttgta ggcctttttg atttcacgct cttccgctgt tttggaaacg cctaaaatct
     1561 cgtaataatc ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag
     1621 aggaaattcc ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg
     1681 tctttgactt cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa
     1741 gcatcagcac cggcagtctg ctgctgggca tgttgctgct gggcgatttc aatcagtttc
     1801 tgggaaacct gtgccagctc ctgcattttc gcttcgatag cggctttgtc ttcacctttc
     1861 agagcagttt ccagtgcagt cagtgcagac tcgatagcag ttttgtcgtc agccggcagt
     1921 ttgtcgcctg cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg
     1981 cgagcctgta ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt
     2041 accattttct ggatttcatc ttcgttcaga ccagaagaag ccttgatggt gatcttctgc
     2101 tctttaccgc tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg
     2161 aaggtaactt cgatctgcgg catgccgcgc ggtgccgggt tgataccatc caggttgaac
     2221 tgacccagag atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt
     2281 accgcagact ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg
     2341 gtgtttttcg cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc
     2401 ggggtaacgt ccagcagcag tacgtctttc acgtcaccag tcagaacacc accctgaaca
     2461 gcagcaccga ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag
     2521 aactcagcaa ctttcttctg aaccattggc atacgagtct gaccaccaac gaggataacg
     2581 tcgtcgatat cagatacgga caggccagcg tcctgcagtg caactttcag cggctcaatg
     2641 gaacggttta ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc
     2701 atgtgtttcg gaccggtcgc atctgcagtg atgtacggca ggttaacgtc ggtctgctga
     2761 gcggaagaca gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc
     2821 ggatcgttgc gcaggtcaat gccctgatct ttcttgaatt cttcaaccag gtagttgatc
     2881 agacggctgt cgaagtcttc accacccagg tgggtatcac cgttggtagc cagaacttcg
     2941 aaggtttttt cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa agtaccacca
     3001 cccaggtcat aaaccgcgat agtacggttg ccagtacctt tgtccagacc gtaagccagc
     3061 gcagctgcgg tcggttcgtt gatgatacgt tttacttcca gaccagcgat acggcctgcg
     3121 tctttggttg cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca
     3181 gttaccggtt cacccaagta atcttcagcg gttttcttca tttttttcag cacttcagca
     3241 gaaatctgcg gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca
     3301 gcagcaataa ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag
     3361 cggcggccaa tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga
     3421 cgtttagccg gctgaccaac cagagtttca ccatcctggg tataggcaat aatagaaggc
     3481 gtggtgcgat cgccttcggc gttctccagc acgcgaggag tggtgccatc cataatcgct
     3541 acacaagagt tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca
     3601 ctatatattc ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca
     3661 actgcccaat ttcatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg
     3721 taacccacgt catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg
     3781 cgatatagca ggacaagacg tgaaactgac agcaatttta ttcatataaa gaatgaatcg
     3841 cagccaagac attcatcaaa aaattgtaat aaaaagaaaa cattacgcac ctgaatcttc
     3901 tctttatcag cagtaaacta gtgggtatcc atccccctac ctctccccac taagagaatc
     3961 cttatgaaat ccgtttttac gatttccgcc agcctggcga ttagtctgat gctgtgctgc
     4021 acggcgcagg caaacgacca taaaatcctc ggcgtcattg cgatgccacg taacgaaacc
     4081 aacgatctgg cgctgaaact tcctgtttgc cgaattgtta aacgcataca actctctgcc
     4141 gaccatggcg atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc
     4201 agtcagagcc tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac
     4261 attaacagcg ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg
     4321 gtgaactcat cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata
     4381 gaagccacaa aaaatgaatg ttaattacct gaatgattca gatctggatt ttctccagca
     4441 ttgcagtgag gaacagttgg caaatttcgc ccgattgctc acccataatg aaaaaggcaa
     4501 aactcgcctc tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg aagggcatcc
     4561 cgagcaacat cgccgcaact ggcagctgat tgctggagaa ttacagcatt ttggtggcga
     4621 tagtatcgcc aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt
     4681 ttcaaaacga ttgaagctga aagccgacaa agagatgtct acgtttgaga ttgagcaaca
     4741 gttactggaa caatttctgc gtaacacctg gaagaatatg gacgaggaac ataaacagga
     4801 gtttctgcac gcggtcgatg cgagggtgaa tgagctggaa gagctgctgc cgctgctgat
     4861 gaaagacaaa ttattggcaa aaggcgtgtc gcatttgctt tccagccaac tgacccgcat
     4921 tttacgcacc cacgcagcaa tgagcgtact tgggcatggt ttgctgcgcg gcgcggggct
     4981 gggaggccct gtaggtgcgg cactaaatgg ggttaaagcg gtcagcggca gcgcctatcg
     5041 cgtgacgatt ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca
     5101 ggtctgatac cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg
     5161 ccccactggc tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat
     5221 ttcaccattc attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc
     5281 cggcaccgct gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg
     5341 tgggttattt cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg
     5401 cgcaaatttt tgctggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat
     5461 tcacctctta cggttctttc tggctgacgc tggttgcgat tctgctgatg ccgaaactgg
     5521 gtctgaccga tgcgccaaat gcacagttcc ttggtgtcta tctgggtctg tggggcgtat
     5581 ttacgctgtt tatgttcttc ggcacgctga aaggcgcacg cgttctgcaa ttcgttttct
     5641 ttagcctgac cgtgctgttt gccctgctgg cgatcggtaa cattgccggt aacgccgcaa
     5701 tcatccactt tgccggctgg attgggctga tctgcggtgc cagcgcaatc tatctggcga
     5761 tgggtgaagt actgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact
     5821 aattgttttg agatcccccc gctcgggggg atttttttat tcgctaacgt cgcgtcttgc
     5881 actcttcggt ctgaatgctg caaccacttc cggtgccgtt tcaacgtatg gcccttccag
     5941 caactgaatg cagtacggta cgctggcaaa aataccgtgt accacaacgt tacccgcagc
     6001 gtccttcaca ccttccagcg tctctttaat agacttcggc tgaccgggta agttaaggat
     6061 cagcgcctgt ttgcgaatca caccaacctg acgcgaaagg atcgcagttg gtacaaaatg
     6121 caggcttatc tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg ctactgccag
     6181 cgtcgcatcg ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg tgagcaccag
     6241 atggcaactc atttcatcca ctagctcaca caacgtttgc tcgatgatcg cctgctcatc
     6301 ggggattaag cgggtttcca gttcaaacgg cgtggttagc gccgatgtca gccattcttc
     6361 cagcgcaggg atgcctttat cctgataaac gccgctggat gcgcgatcag agatggaaac
     6421 taagccaata cgtaaagtat tcatcaagat ttttccgttt aaacagtctc gttaaacaga
     6481 atgatacact gcgaagggag tgacagacag gcttcagaag aggtagcgtg accgacttcc
     6541 cggtcacgct aagaatgatt acagcagatc gccgatcatt ttttccagtt tttcctggtc
     6601 aatagcaaac ttacggatac cttccgccag tttatctact gccattggat cctggttgtg
     6661 ctgccacagg aactcggact cagtgatacg cgccggacgc gctttcactt cgccggtgta
     6721 agacagttta cgttcgatag ccccttcgct ctcagccagc tctttcagca gtgccggtgc
     6781 gatggtcaga cggtcgcagc ctgccagttc cagaatttcg ccgatgttac ggaagcttgc
     6841 gcccataacc acggtttcat aaccgtgctc tttgtagtac tggtagattt cagatacaga
     6901 aaccacgccc ggatcttctg ccggagcgta ctctttctta tcggtattcg ctttgtacca
     6961 gtcaagaata cggccaacaa acggcgagat caggaacacg cccgcttccg cacaagcacg
     7021 agcctgagcg aaggagaaca gcagggtcag gttacagttg atgccttctt tttccagctg
     7081 ttctgcagca cggataccct gccaggtaga agccagtttg atcagaatac gatcgttgct
     7141 aataccagca tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg acgcttcagt
     7201 gtcataggaa agacgcgcat caacttcagt tgagatacgg cccggaacca gtttcaggat
     7261 ttccagacca atatttactg ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc
     7321 gttgctctgc tgtttcgccc aggcgacagc atcatcaatc aacttacggt attccggaat
     7381 ctgcgctgcg ttaagaatga gagaagggtt ggttgtggca tcctgcggtt gatacagctt
     7441 cattgccgcg atgtccccag tgtcggccac tacggtggtg tactgacgaa gggaggtcaa
     7501 tttgtccgtc atgatagtat ttctctttaa acagcttgtt agggggatgt aaccggtctg
     7561 ccctgatgat atcacgacgc aacgtcagcg caaccgccga caagacggtt atcgccaggc
     7621 gagactgttt cggatttctg acactgcgtt gatatcgccc gccattttta tacaaaacct
     7681 catgtatgct acgcagaagt tatcaagtac ctcgtagcgt atatacttct taaacaattg
     7741 gtaacgttta cacaggaaag tcatcgcgac cggcaataag agggatatgc atgccagatt
     7801 ttttctcctt tattaacagc gtcctttggg gatcggtaat gatttacctg ctcttcggcg
     7861 caggttgttg gttcactttt cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg
     7921 gcaaaagtct taaaaatagc attcatccac agccaggcgg tttaacctca tttcagtcat
     7981 tgtgtaccag tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt gcgctggcaa
     8041 ttaccgccgg tggacctgga gccgtctttt ggatgtgggt tgccgcgttt atcggcatgg
     8101 cgacctcgtt tgccgaatgt tctctggcac aactttataa agaacgtgac gtcaatggac
     8161 agtttcgtgg cggaccggca tggtatatgg cgcgcgggct ggggatgcgt tggatgggcg
     8221 ttctgttcgc cgtctttttg ctcatcgcct atggcataat tttcagcgga gttcaggcga
     8281 acgccgttgc ccgcgcccta agtttttctt ttgattttcc tccgctggtg acaggcatta
     8341 ttctcgctgt ctttgctctg ctggcaatca ctcgcggtct tcatggcgtc gcccggctca
     8401 tgcagggttt tgtcccgttg atggcgataa tctgggtact gaccagcctg gtgatttgcg
     8461 taatgaatat cgggcaactt ccccacgtca tttggtctat ttttgagagt gcttttggct
     8521 ggcaggaagc ggcaggcggc gcggcgggat ataccttaag ccaggcgatt actaacggtt
     8581 ttcagcgcag tatgttttcc aatgaggcgg gaatgggttc tacaccaaac gcagcagcgg
     8641 cagcggcgtc ctggcctccg catccggcag cgcaagggat tgtccagatg attggcattt
     8701 ttatcgacac cctggtcatc tgtacggcaa gcgccatgct gatattactg gcgggtaacg
     8761 gcacaaccta catgccgctg gaaggtattc agcttatcca gaaggcgatg cgggtgctaa
     8821 tgggttcctg gggtgctgag tttgttaccc tcgtggttat tctgtttgcc ttcagctcca
     8881 tcgttgccaa ctacatttat gccgaaaaca atctcttctt tttacgcctg aacaacccta
     8941 aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt catcggcggc accttgctaa
     9001 gtcttccgct gatgtggcaa ctggcagata tcataatggc ctgcatggct attaccaatt
     9061 tgaccgccat tttactgctc tcgcctgtgg ttcataccat tgccagtgat tatctacgcc
     9121 agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat atcggtcgcc
     9181 agctttctcc ggacgcgtgg gatgatgttt cgcaggagta atcacaacta tcgatcaact
     9241 cattctcttt ttttgctaaa gtcggcataa atttcctgca aggactggat atgctgattc
     9301 ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt gaccaccatg cgctatacgc
     9361 tgccggagct gttagacaat tcccagcagt tgatccatga agcgcggaaa ctgacgcctc
     9421 cgcagattag cacgctgatg cgcatcagcg acaaactggc gggtatcaac gccgctcgct
     9481 ttcatgactg gcagccagat ttcacgccgg agaatgcccg ccaggcgatt ctggcgttta
     9541 aaggtgatgt ctacaccggc ttgcaggccg aaaccttcag cgaagacgat ttcgattttg
     9601 cccaacagca tttgcgaatg ctttccggct tgtatggcgt actgcgcccg ctcgatttaa
     9661 tgcagcctta tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg aaagatctgt
     9721 atcaattctg gggagatatc atcaccaaca agctgaacga ggcgctcgca gcacaaggcg
     9781 ataatgtggt gattaatctg gcgtcagatg agtattttaa atcggtgaag ccgaagaaat
     9841 tgaatgccga gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa tttaagataa
     9901 tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg ttttattatt gaaaatcggc
     9961 tgaccaaacc agagcaactg actggtttta atagcgaagg ttacttcttt gatgaagctt
    10021 cctccagcaa tggcgaactg gtgtttaaac gctacgagca gcgttaagtt tgtagtcaat
    10081 gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc atgcagattc aatatattgc
    10141 agaattcacg taggcctgat aagacgcgcc agcgtcgcat caggcgctga atgccggatt
    10201 gttacccggc atttgtcatt tagcggtgat gtttgcctgg accatgaccg ccgtgatgat
    10261 catgaggagc tttcttatgg tggcgcggcg gtggcggcgg tccgtgtggg tgccagcgat
    10321 tgcctcgcca ttcataatgt tgtttccacc agccgtggtc gcgccagtga cctccatccc
    10381 agtaatagcc acgattatca cgatcgccta tctgtaattt tactgacggg actaacgtaa
    10441 tttccgcagc ctgtgctgcc atgggagcga ccagaaccag ggaaagtgcg agtacgatag
    10501 attgcatctt tttcacaggt tactcctttt atcattgccc gttatgggtc gatatccgga
    10561 gattacgcga tgagaacgac ggttgttatt ctccgcaatc ctaatctcta agttaccgca
    10621 tttattgaga atttctcctt tttccctgtc gtttttctcc ataatttctt cataaaaaag
    10681 ccgggctgca taaaagcaaa cccggcctga ttgagataat gaatagattt tactgatgat
    10741 tcatcatcaa tttacgcaac gcagcaaaat cggcgggcag attatgtgaa agcaagggta
    10801 aatcagcacg ttctgccagc tcttttggca gatccaacgt ttcaccgaga atcgcttcca
    10861 cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat tcgcctggat
    10921 tcaactggtc acgcagcgca cgatacgcta cggcagcgtg cggctccgag gtgtagccca
    10981 gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc atccacggct gcataaccca
    11041 gctctttcag ttgccagatt ttgcggcgga acaactcttc cacacgcggc cagttgttcg
    11101 gctgactcac gtccatcgcg ttggataacg tcgcctgagt cgctttgggt gaccactgac
    11161 cgtcgtgcag gaaacgtggc acggtatcgt tcacgttggt cgcagcaata aaacgtttca
    11221 ccggcagacc gagtgacttc gccagcagac ccgccgtcaa atcgccgaag tttccgcttg
    11281 gcaccgagac aaccagctgg ttgcgcgctt cctgcggcag ctgcgcaaca gcttcaaagt
    11341 agtagcaaat ctgcgccagc aaacggctga tgttaatcga gttagctgag tttaacccca
    11401 gcgccacttt cagttcttca tcatcaaacg cctgcttcac cagcgcctga caggcatcga
    11461 aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa cgtacagaac agtttttctt
    11521 gcagtggact gattttgcct cgtggataga ggataaccac tttcacattc ggtaaaccgt
    11581 agaaagcatg agccactgcc gctccggtat caccggaggt cgcggtcaga atggtcactg
    11641 gcttatcgcc cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg aaatctttaa
    11701 atgccagcgt tggcccgtgg aacaattcca gacaaccgac atcgctttca acattggcga
    11761 ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc tgcgggattt
    11821 catcaccaat aaacgccgag aggatcttcg cactgcgggt gacaaaatcc agcttcagca
    11881 tctcatcaat ttcagtcagg ctgaattccg gcaggtcatg cggaaaaaac agcccctgat
    11941 ttttgcccaa cccctgggtt acggcttgcg caaagctgac ctgctcgttg tgatctttca
    12001 gattgtagag tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc agccggcaaa
    12061 tatgaacaaa accttcctga ttttgcaggt agttcttacc caaccagtcg gcaacgcgct
    12121 gggcggtatc cggcttgtca catagagcga acaaggtcgg gccggagccg gagataccgc
    12181 tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg ccggaagcca ggcagtaacc
    12241 gttcacggta gggttcagcg ataacatctt tcatcagctt cgcggcaagc tcaggctgac
    12301 gggaatagca ggcgtgaatg aagccagcca gatgtcgccc gtgcgcaatg caatcctggc
    12361 ggcgatactg cgccggtaaa atagccctgg cttctgccgt cgagacttta atccccggat
    12421 acgccagcac ccacagccac tcatcaaacc ctggcacttg ctggctgatg atgtcgtttt
    12481 cttcgatcat caactgcata ccaccaagaa aacacggtgc cacgttgtcg taatgaatgc
    12541 tgccggagat tcgtccttcc agctcgccca tcaaagccag caaacgagtg tcattaagcg
    12601 gcttgccaca gtgttcattc atcgccatca gcgccgcgac caccgaacag gcgctggagc
    12661 ctaagcccga accgattggc atattctttt ccagagtcat cgccactgga atttgcttgc
    12721 caagctcctg gcaaaaacgc tcccagcact gataaacgat attttcccgt ggttctgacg
    12781 gtagcttatc ggcaaagcgt ccgaggttgt tgagactgaa tgtctctgcc gcctcaaccg
    12841 tgactacatc tccgagcaat gcaccatcaa caggtgtcac cgccgccccg agcacatcaa
    12901 acccgacgct catattggca ctggaagccg gggcataaac tttaaccatg tcagactcct
    12961 aacttccatg agagggtacg tagcagatcg gcaaagacac cggcagctgt aacgtcattg
    13021 cccgcaccat atccgcgcag caccaacggc agcggctgat aatagtggct ataaaaggcc
    13081 agggcgtttt cgccattttt cactttgaac agcggatcat taccatccac ttcggcaatc
    13141 ttcacgcggc aggcaccatc ttcatcaata ttgccaacat agcgcaaaac ttttccttca
    13201 tcacgggcct tcgccacgcg cgcggcaaag agatcgtcga gctgtgacag attcgccata
    13261 aaagcggcaa catcaccctc agcgttaaac tctgcgggca gcacaggttc aatttcaata
    13321 tccgccagct ccagttcacg tcccgtttca cgagcgagaa tcaatagctt acgcgctaca
    13381 tccataccag aaagatcatc tcgcggatcc ggttcggtat aacccatttc ccgcgccaga
    13441 gtagtcgcct cggagaaact catgccttcg tctaacttgc cgaagatata agaaagcgaa
    13501 cctgaaagaa tgccggagaa cttcatcaat tcatcaccag cattgagcag attttgcagg
    13561 ttctcaataa ccggtaatcc agccccaacg ttggtgtcat agaggaattt acgccgcgat
    13621 ttttccgccg catgacgcaa cagatggtag taatccatcg acgaggtgtt ggcctttttg
    13681 ttcggcgtga caacgtggaa accttcgcgc aagaagtcgg catattgatc cgccactgcc
    13741 tggctggagg tgcagtcaac aatgaccggg ttcagcagat gatattcttt cacgaggcga
    13801 attaagcgcc cgagattaaa cggctctttg gcttgcgcca gttcttcctg ccagttttcc
    13861 agatttaggc catgcacatt ggtgagcagt gccttcgagt tggcaacacc gcagacacgt
    13921 aagtcgatat gtttattctt cagccagctt tgttgacgct tcagttgctc cagcagcgca
    13981 ccgccaacgc caccgacgcc aatcacaaac acttcgataa cctgatcggt attgaacagc
    14041 atctgatgag taacgcgcac gccagtggtc gcatcatcgt tatttaccac gacagagatt
    14101 gagcgttcag aagatccctg agcaatggcg acaatgttga tattggcgcg ggccagcgcg
    14161 gcaaagaatt tcgccgagat cccacgcaag gtgcgcatac catcacctac caccgagata
    14221 atggccagcc gttccgtcac cgccagcggc tccagtaagc cttctttcag ttccaggtag
    14281 aactcttcct gcattgcccg ttcagcccgc acacagtcgc tttgcggaac gcagaaactg
    14341 atactgtatt cggaagatga ttgcgtaatc agcaccacgg aaatacgggc gcgtgacatc
    14401 gcggcaaaga cgcgcgccgc catgccgacc atccctttca ttcccggacc agaaacgctg
    14461 aacattgcca tgttattcag attggaaatg cccttgaccg gtaattcgtc ttcatcacgg
    14521 ctggcaccaa tgagcgtacc tggtgcttga ggatttccgg tatttttaat caggcaaggg
    14581 atctggaact gggcgatggg ggtaatggtg cgggggtgaa gaactttagc gccgaagtag
    14641 gaaagctcca tcgcttcctg gtaggacatc gacttcaaca acctcgcatc gggcacctga
    14701 cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca atcggcgcgt
    14761 aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt tgcgtccaag taccaccagt
    14821 tcgccttttt cattaccggc ggtgaagcct gccatcagca ccatgtgatc agccggaata
    14881 cgacttgccg caatacggcg ggtggactca gcaatatcga cggtagattc gaggtaatgc
    14941 cccactgcca gtagtttttc gaccggatcg ataacggtaa cgttgtgacc acgcgcttct
    15001 aacacgccgg ccataatggc gatcgacatt ttctcgccac ggcaaatcag cgcagcgttg
    15061 atgctatccg ggcactgccc caacaaacta atgccatgca ggacatgttt tatttgggca
    15121 aattcctggt cgacgaaagt tttcagttgc gccagcggga atcccggctg ggcggcggtg
    15181 agtcccgtca gaagttcggc aaaaatacgt tcggcatcgc tgatattggg taaagcatcc
    15241 tggccgctaa tggttttttc aatcatcgcc accaggtggt tggtgatttt ggcgggggca
    15301 gagaggacgg tggccacctg cccctgcctg gcattgcttt ccagaatatc ggcaacccgc
    15361 agaaaacgtt ctgcatttgc cactgatgta ccgccgaact tcaacactcg catggttgtt
    15421 acctcgttac ctttggtcga aaaaaaagcc cgcactgtca ggtgcgggct tttttctgtg
    15481 tttcctgtac gcgtcagccc gcaccgttac ctgtggtaat ggtgatggtg gtggtaatgg
    15541 tggtgctaat gcgtttcatg gatgttgtgt actctgtaat ttttatctgt ctgtgcgcta
    15601 tgcctatatt ggttaaagta tttagtgacc taagtcaata aaattttaat ttactcacgg
    15661 caggtaacca gttcagaagc tgctatcaga cactcttttt ttaatccaca cagagacata
    15721 ttgcccgttg cagtcagaat gaaaagctga aaaatactta ctaaggcgtt ttttatttgg
    15781 tgatattttt ttcaatatca tgcagcaaac ggtgcaacat tgccgtgtct cgttgctcta
    15841 aaagccccag gcgttgttgt aaccagtcga ccagttttat gtcatctgcc actgccagag
    15901 tcgtcagcaa tgccatggct cgttcgcgta aagcttgcag ttgatgttgg tctgccgttg
    15961 tgtcactttt cgccggttgt tgtattaatg ttgctaattg atagcaatag accatcaccg
    16021 cctgccccag attgagcgaa ggataatctg ccaccatcgg cacaccagta agaacgtcag
    16081 ccaacgctaa ctcttcgtta gtcagcccgg aatcttcgcg accaaacacc agcgcggcat
    16141 ggctcatcca tgaagatttt tcctctaaca gcggtaccag ttctactggc gtagcgtagt
    16201 aatgatattt cgcccgactg cgcgcagtgg tggcgacagt gaaatcgaca tcgtgtaacg
    16261 attcagccag tgtcgggaaa actttaatat tatcaataat atcaccagat ccatgtgcga
    16321 cccagcgggt ggctggctcc aggtgtgcct gactatcgac aatccgcagt tcgctaaaac
    16381 ccatcgtttt cattgcccgc gccgctgccc caatattttc tgctctggcg ggtgcgacca
    16441 gaataatcgt tatacgcata ttgccactct tcttgaccaa ataaccgcga acagggtgat
    16501 cactgtcaac ttattacgcg gtgcgaattt acaaatcctt aacgtaagtc gcagaaaaag
    16561 ccctttactt agcttaaaaa aggctaaact atttcctgac tgtactaacg gttgaattgt
    16621 taaaaaatgc tacatatcct tctgtttact taggataatt ttataaaaaa taaatctcga
    16681 caattggatt caccacgttt attagttgta tgatgcaact agttggatta ttaaaataat
    16741 gtgacgaaag ctagcattta gatacgatga tttcatcaaa ctgttaacgt gctacaattg
    16801 aacttgatat atgtcaacga agcgtagttt tattgggtgt ccggcccctc ttagcctgtt
    16861 atgttgctgt taaaatggtt aggatgacag ccgtttttga cactgtcggg tcctgaggga
    16921 aagtacccac gaccaagcta atgatgttgt tgacgttgat ggaaagtgca tcaagaacgc
    16981 aattacgtac tttagtcatg ttacgccgat catgttaatt tgcagcatgc atcaggcagg
    17041 tcagggactt ttgtacttcc tgtttcgatt tagttggcaa tttaggtagc aaacatgcag
    17101 accccgcaca ttcttatcgt tgaagacgag ttggtaacac gcaacacgtt gaaaagtatt
    17161 ttcgaagcgg aaggctatga tgttttcgaa gcgacagatg gcgcggaaat gcatcagatc
    17221 ctctctgaat atgacatcaa cctggtgatc atggatatca atctgccggg taagaacggt
    17281 cttctgttag cgcgtgaact gcgcgagcag gcgaatgttg cgttgatgtt cctgactggc
    17341 cgtgacaacg aagtcgataa aattctcggc ctcgaaatcg gtgcagatga ctacatcacc
    17401 aaaccgttca acccgcgtga actgactatt cgtgcacgca acctgctgtc ccgtaccatg
    17461 aatctgggta ctgtcagcga agaacgtcgt agcgttgaaa gctacaagtt caatggttgg
    17521 gaactggaca tcaacagccg ttcgttgatc ggccctgatg gcgagcagta caagctgccg
    17581 cgcagcgagt tccgcgccat gcttcacttc tgtgaaaacc caggcaaaat tcagtcccgt
    17641 gctgaactgc tgaagaaaat gaccggccgt gagctgaaac cgcacgaccg tactgtagac
    17701 gtgacgatcc gccgtattcg taaacatttc gaatctacgc cggatacgcc ggaaatcatc
    17761 gccaccattc acggtgaagg ttatcgcttc tgcggtgatc tggaagatta atcggcttta
    17821 ccaccgtcaa aaaaacggcg ctttttagcg ccgtttttat ttttcaacct tatttccaga
    17881 tacgtaattc atcgtccgtt gtaacttctt tactggcttt cattttcggc agtgaaaacg
    17941 cataccagtc gatattacgg gtcacaaaca tcatgccggc cagcgccacc accagcacac
    18001 tggttcccaa caacagcgcg ctatcggcag agttgagcag tccccacatc acaccatcca
    18061 gcaacaacag cgcgagggta aacaacatgc tgttgcgcca gcctttcaat accgcctgca
    18121 aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc acggtaaaac
    18181 cggtatgttc agaaagcgcc agcaagagca aataaaacat caccaatgaa agccccacca
    18241 gcaaatattg cattgggtgt aagcgttgcg cggtgagcgt ttcaaaaaca aagaacgcca
    18301 taaaagtcag cgcaatcagc agaatggcat acttagtcgc ccggtcagtt aattggtatt
    18361 gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat
    18421 tgcctgaagc aaaacgctca ccgagattat ttgcaaacca gctgctttgc cattgcgcct
    18481 gaaaacctga ctcgctaact tcccgtttgg ctggcaaaaa atcacctaaa aaactgggat
    18541 gcggccagtt gctggttaag gtcatttcgc tattacgccc ggcaggcacc acagaaagat
    18601 cgccggtacc gcttaaattc agggccatat tcagcttcag gttctgcttg cgccagtccc
    18661 cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccggta ccgggttcga
    18721 tggtcagcgc tgttccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc
    18781 cgacgctaat cacaataaat ggcttgccta aggtgatatt tggtgcgtcg agttcgctaa
    18841 gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat
    18901 aaatccctat cttgcgttct tcaacgttct gattgccatc aaccatcaat gactcaggta
    18961 accaaaaatg gataaaactt cgtttccgct ccacggtttt atcgtcttcc tgcaccgtat
    19021 aaagctcggt cacaggaata gcgatgagcg gcccaacgag tttttgcggc ccgctggtac
    19081 tttggcgaat cgcatcttcc acatcgctac ggtaatcggc acgttcgaca atcacctgcc
    19141 gaatcagcat tatcggaatc aacaacagca atactgcacc aaacaaggtg gtcattttcc
    19201 agaacagggg ggatttcaac atagtcttct cctttgcaat ggcagcagga tacgaaggct
    19261 atgtgggaag aatttgaagc tatgtgaagt gacggtgaag tcgtagcgag gccagcacgc
    19321 caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga
    19381 cgaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc
    19441 gttcaaaaat acgtgacagc gcgtagtcag gaataccact accggtatcc agcaccttaa
    19501 gggcgacgtg ttcctgatcc acttcggcgc ttagcgttat gcgaccgctc tcgggggtaa
    19561 aatcgatggc gttatccagt aaattcccca gcgcctgctc cagtaacgcc ggttcagcag
    19621 caacattaac ctcagtgggc ataacatgta gagtgatgtt tttttctgcc aactgcacgg
    19681 tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc aacaacagtc agaacgactt
    19741 cctgacgatt ctccagtctt gcctggcgta gtaacgtttc taccaatgcc tgcatacgcg
    19801 cattttgcgt cagaatgttg tcggtaaaac gagccaccac ttccggtggc ggaccttcgc
    19861 gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgggttaacg
    19921 cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg
    19981 cctgcgcaag cttacgcaac tcgctactgc cgagttcagg aagaggaacg ggcttattgt
    20041 cagtgacgga atcggcatag cgagtgagcc gggcaatgga gcggttgatc caccaaacca
    20101 tgcctgcgcc aatcaccagt gcaatcccca gcaaaatggc gctggcccat aaaattcgcc
    20161 gctcgctacg cttaatgacc ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc
    20221 caataagccg cgagccgtcc ataatcggcg cggcaacata catcaccgaa ctttcgggat
    20281 cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt
    20341 cattccagcg cgaataatcc tgcccaacgg ctttatttgc cgaatcgaac aatactttac
    20401 cctgcgaatc ggtcatatag acatgatatt cattgcgcac tttgttaatg ccaccgatat
    20461 tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg
    20521 ggtccccaga gagcaaatcg ggacgcgcca gctccgccag caacgttgcg gtatcgatta
    20581 acgtcccctc cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagta
    20641 cgaaccaggc cgccaccgcc accagtaaaa aatagcccag cagcaaccgc atgccgatac
    20701 gcattacagg cccctcaggc tatatcccat gccgcgatga gtattaatcg gtgaaagatc
    20761 ggggttgatg gcgcgcagct tggcacgcaa cgttttaatg tgggtatcga cggtgcgatc
    20821 gtaggtgtcc tgcgcatctt cccacacgct atccattagt tgctggcggg accagacgcg
    20881 gcctggcgac ttgagtaacg tcttcagcaa taaaaattca taccgcgtca gcgttaatgg
    20941 cgtgtcaaac cagctgatct gcgccgcggg ttcattcaat tcaaaatgac caatacggat
    21001 gacgggagac ggcgacgaga acttcttcac ccgacgcagt aaggtgcgca ccctggcgca
    21061 cacttcgcgg ggtgaaaacg gtttagccac atagtcgtca gcgccaattt ccagcccaag
    21121 caggcgatcg acctcttcac ttcgggccgt caggaacagt acaggtaacg ccggatggag
    21181 cgccagtaac tggcggcaca attcaaagcc gctgatatca ggcaaaccaa catcgagaat
    21241 catgacgtcg ggaacctgct ggcgagcttt atccagcacc ggcaagcctc gctcaaagac
    21301 ctcgacggca aaaccttcct gctgcaacat gtagaccagc gtgtcggcta tcccttgctc
    21361 atcttccact aaccagaccg tttcccgttg cataaatcgc ctctgttatt gccgccacgg
    21421 catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta
    21481 agccagataa gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct gtaacgactt
    21541 aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat
    21601 acgatccgac agttcaatcg gcccgacttg ctgacaagaa atagccgcat cggaggtatc
    21661 ttccgccaga cccaatcccc ctttaatacc acctgttttc gcccggctca cataacaggt
    21721 gacatttttc acatcgggat cgtcaaaggc ttccacaaca attttgtgat ccgggccgat
    21781 cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg gccccagcat
    21841 tatcaggctt aaagacagga tcaaatgctt gtatttcata ttgttaccat ttttatctat
    21901 tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaata
    21961 cttttatttc cgtcaagacc taaaacatac tctactaaga aaaaaacact gaatgctaaa
    22021 acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga
    22081 ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat
    22141 ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac
    22201 ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt
    22261 cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg
    22321 ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa gcagtttgcc
    22381 cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg
    22441 ctacgtctgg gtgaattcac aatgccagag cacaaatttg tcaccctgga agatacgccg
    22501 ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat
    22561 gaaatgcgtt atcagttctg gcacgatttt ctcggcaacg cgccgaccat tccgccagtg
    22621 ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgacgaaca agaggtattc
    22681 tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg gcatccggtg
    22741 atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag
    22801 gagtttatcc tgacagtata cggaacgtgc atgccaatgc tcaacctgac gcgccgtaaa
    22861 ggtcaggata ttgagcgata ctacccggca gaagacgcca aagcgggaga tcgcccaatt
    22921 aatctgcgct gtgaactgct gattcctatc cgccgttaac gctgcagctc atctaatgca
    22981 ggggcgtcca gatgcgagat gtcccctgcg gtttcaacga cccagccaga cgccagccac
    23041 aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct
    23101 gcccatgctg gtaatccgag aatcgtactc accaggcatc ccagtgcaat accatgactc
    23161 accagcagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattgacg
    23221 cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac ggtgccattg
    23281 accagctgcc gacgccagtt ctcttcttct tcggtcagag aatcgatatg tcttttttcc
    23341 agcacaccca tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc
    23401 tgggcgatga tttccgccgt acgccgggtg cgtcctaaat cgctactgat gatatgcgta
    23461 atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc tttggcggtc
    23521 agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg
    23581 cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct
    23641 gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg
    23701 ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccgttg
    23761 ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc gctggcgcac
    23821 gaaatcgggt agccaatgcc cgccgtttac ttccagaggc tgatttttgg gtggcgattg
    23881 aagctggcat cgatggtgac agcactttca gttgggtggt gattgaaaac gccagccagc
    23941 gcggcgaggc gcgttcggct actttgccgt tgcctgcggt gattctggaa aaagtgcgtg
    24001 aaggtgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag attggtcgta
    24061 aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc gtctatcacc
    24121 aggcggtgat cctggcgctg agtccgtttc ataatgccgt gtattaagcg cctgatgcga
    24181 cgctgccgcg tcttatcatg cctaccaaac atattgaaat tacgggtatt tgtaggacgg
    24241 ataaggcgtt cacgtcgcat ccggcattcg gtgcacgatg cctgatgcgc cacgtcttat
    24301 caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactgg cgcagctcga
    24361 cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgcct gcgccgagtt
    24421 cattttttaa ctcacgctgg ctcatttcgc cgcgcaacag ctcttcgaca atacgcacgc
    24481 gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac ggtaaatgga
    24541 gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc tggtgacgct
    24601 gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc cattgttagc
    24661 gggggaagca aaatgcctcc cccggttgta ctcgctaaag agtacgatag catatcataa
    24721 acgtgcggat cagtaacgac gtccccattc cgtggcgctc atcaacgtcg gtttatcccc
    24781 catgaaatag cggtagtaag cgtcataagc cagcacgttc ttcacataac cgcgcgtctc
    24841 ggagaatgga atactctcga caaatgccac tgcgtcgata cgcccggcgc tgttgccaag
    24901 ccaggttcgc acccgccctg gtccggcgtt ataagctgct gaggagaaaa tacgattatt
    24961 gccaaactgc tgataaacat attgcaggta actggtgcca atgttgatat tcgtttccgg
    25021 atccagcaat tgcccaggac tgctataacc gggaatagag aacatcttca ccgtatgggt
    25081 cgctgtacca ggcataatct gcatcaagcc gctggcccct accggtgatt tcactttcgg
    25141 attccaggcg ctctcctgac gagcaatcgc catcgcatag ctttgcggga tctccttacc
    25201 gctggtgtag cgtttgaaaa gatcgttgta agccagcggg aatcgctctt ccagatgatc
    25261 ccacagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt tgttgaaagc
    25321 ataccgcgcc agttgagcct gctctgtttt tgacttgctc ttcaccagat tggcccactc
    25381 gctacgcgcg gtattatcga gattccagta catcaactcg cgcacgcgcg ccatctccgg
    25441 cccctgagtc agggcgctgt caacattctg cggcgcttta tcaatcttca gctcatactc
    25501 ttcgccgatg cgttgtgctg caaccatcgg gtagaaacca cgctgttgca tgagttgatg
    25561 caaaatctct tttgcttcag cttcacgtcc gcgttccagc aataaatccg cctgccagta
    25621 acgccattca tctttctctt tcgcttccat aggcagacgc gccagccagg tattcaggcc
    25681 gcgacgatcg ccggtgccaa gcgccattcg tacacggcgt tcaataagcg aagtagattg
    25741 cgagcgcata atggcgtcat cgcgccattt cgcctgctcg tcggtgacat cgttgcccat
    25801 caaacgccag gcgacgatat cgcgcagctc ctgaatctga tcttcattaa gctgctgcgc
    25861 ctgggcaagc gatgggatca tcagccgtgc attctcagca tcctgccgcg ccacactggc
    25921 aaacgccacc gccgccattt gacgggtaaa atcggtcgcg ccagttgtac gcgcgaaggt
    25981 cagtaccgta ttagggttgt tcgccagtga aatgattgcc gaggcgatag tctggtaatc
    26041 ggcaggcatc tgccctgcca gcactgttac caggcctgtg ttacccgctt tcatcgccag
    26101 acggatacgc tctaaatacg ccagcggatc ttgtttacct gacgcacgcc agacgctaaa
    26161 taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt tcgccccttg
    26221 ccaggcttct tcactctgcc cgatgttcca tttcgcatag tagtaattac attgcgcttc
    26281 ggtagttccg ggcttttccg ggctaaaggc taacaagcca cgccagtctt cacgccgcgc
    26341 cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg ggttagcgcg
    26401 aacaaagtta gtgaccgtca ccgccggttg attcatcaga tcatcggtga tctggcggta
    26461 ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt cgaccacatc
    26521 catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa cgactacgct gctcatccag
    26581 tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag ccaacagccg
    26641 ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat acatcaatgc
    26701 agcgtcatgc cgcgtaatag atttatgcta accagtcatt gccgtttacg ccacgttacg
    26761 gacacttttt tacttttact gcgagggtga tcgaccggct gggattaggc ggggaaaaag
    26821 gctacacttc gcctttgaaa actcattatc acgataaaca tagaggcgaa gtccaacgtg
    26881 gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa acgtcatatt
    26941 ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct gggtctgaac
    27001 ggcgcgggta agtccaccct gctgcgcatt atggcgggca ttgataaaga catcgaaggt
    27061 gaagcgcgtc cgcagccaga catcaagatt ggttatctgc cgcaggaacc gcagctgaac
    27121 ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt ctgaagtggt taacgccctg
    27181 aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt tgacaagctg
    27241 gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca taatctgaac
    27301 gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg actgggacgc gaaaatcgct
    27361 aacctctccg gtggtgaacg tcgtcgcgta gcgttgtgcc gcctgctgct ggaaaaacca
    27421 gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt ggcctggctg
    27481 gaacgcttcc tgcacgactt cgaaggcacc gttgtggcga ttacccacga ccgttacttc
    27541 ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg gtgaaggtat tccgtgggaa
    27601 ggtaactact cctcctggct ggagcagaaa gatcagcgcc tggcgcagga agcttcacaa
    27661 gaagcggcgc gtcgtaagtc gattgagaaa gagctggaat gggtacgtca aggtactaaa
    27721 ggccgtcagt cgaaaggtaa agcacgtctg gcgcgctttg aagaactgaa cagcaccgaa
    27781 tatcagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg tctgggcgat
    27841 aaagtgctgg aagtcagcaa cctgcgtaaa tcctatggcg atcgtctgct gattgatgac
    27901 ctgagcttct cgatcccgaa aggagcgatc gtcgggatca tcggtccgaa cggtgcgggt
    27961 aaatcgaccc tgttccgtat gatctctggt caggaacagc cggacagcgg caccatcact
    28021 ttgggtgaaa cggtgaaact ggcgtcggtt gatcagttcc gtgactcaat ggataacagc
    28081 aaaaccgttt gggaagaagt ttccggcggg ctggatatca tgaagatcgg caacaccgag
    28141 atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca gggtaaacgc
    28201 gttggtgaac tctccggtgg tgagcgcggt cgtctgcatc tggcgaagct gctgcaggtt
    28261 ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga aaccctgcgc
    28321 gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc gcacgaccgt
    28381 tggttcctcg accgtatcgc cacgcacatt ctggattacc aggatgaagg taaagttgag
    28441 ttcttcgaag gtaactttac cgagtacgaa gagtacaaga aacgcacgct gggcgcagac
    28501 gcgctggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt aaaatgccgg
    28561 atgcggcgcg aacgccttat ccggcctaca aaaccagcaa tttcaatacg ttgtaaaact
    28621 gtaggcctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa tgccggatgc
    28681 ggcgtgaaca ccttttccgg tctacaacag ccttagcatc ctcaatttcg tggcgggtca
    28741 cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc ctcttccaga gaggttttca
    28801 actcatcaaa aaaactcatt ttatacctcg cctttgagca gtttcatcac ggttatctct
    28861 gctcccccat catctcccgc accagctcca cgcagcgcag gaaacggctg tcgtaatctt
    28921 cctcttcaac ccgcacaaat tcgatattgt tctcttccag catctccacc agcagattct
    28981 ggaactcttt acgatccacc gaactgccga ggctgcgtaa accatccgcc acccacggcg
    29041 tgttgttctc cagcaggatc accagatcga aacggtattc atcaatcagc gcctgcacga
    29101 acggatgttc ccgcccttcg tactttttgc agaacgcctg agtggtgaca aaatcggtat
    29161 cgataaatgc cactttattg gcatatttca ccgcaaaatc aatgtattga gcgtggccca
    29221 gcgcgatttt atcgtagtca gaatactgca atgcgatctc atcaccgccg aggtgtgaaa
    29281 agacataatc gcggccatat tcccacgcac tggtggtgtt gaagatattg gcaagtttgt
    29341 ttaccagggt ggatttaccg ctcgactcgc cgccaaggat cgccacggta cgcacaaaaa
    29401 acggcttcac ttcggtagga atatattccc agtagcggaa cgggttttcg cggatctgcg
    29461 caccgctgat actcataaag gtacgtttcg gatcgaccag caccgtctcg atccccagat
    29521 gttccatata ctgtggcgca tcggcttctt ccgaggtgta gatcagatcc ggctggatcc
    29581 ctttttcagc cataaacttt ttgataccgt tgctccacac atcccagccg tgcggatacg
    29641 gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt tttctgatat ttaaaagttt
    29701 gcaataacca gcgcagacga tccggcacgg tcggctgctg cgacatggca ctgtcttcga
    29761 acaacgcgcg gtcacgggta tcgtcaaaac ccataatgat atgcagctcg tcaacctggc
    29821 tacaggcgcg ctggataagg tagatatgtc cggtatgcag tgggtagaac ttaccgaata
    29881 cgacaccgat cgttttcttc tgccggggaa actcaagccc caaaaaacgg tgcaacgcct
    29941 ccagcttttg cgcgctgggg cttttgattt tggcattcag taactggctt aaataccctt
    30001 tggtcatacc gctggcatca gctacctgct gtagcgtgca gccctgttgc ttgatggcag
    30061 ttttcaggta atcaaatgac gacatatctc cctccgtatt tctcattata agtcgtcgaa
    30121 cacgctaagc gcgtcggaga gttttttaac gccaaaaatc tgcatccctt ccggcgcttt
    30181 tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg ccgcttcaga
    30241 gattcgttcc tgaccgctgg gcaccgggcg gatctcccct gccagcccga cttcaccaaa
    30301 caccaccaga tcctgcggta gcggtctgtc acgcaggctg gaaaccatcg ccagcagtaa
    30361 cgctaagtcg gcgctggttt cggttacctt cacgccgccg accacgttca caaacacatc
    30421 ctgatcggcc atttgcagac caccgtgacg gtgcaagaca gccagcagga ttgccagacg
    30481 gttttgttcc agccccactg ccacgcggcg cggattcgcc atcatcgagt gatcgaccag
    30541 cgcctgaatc tccaccagca acggacgcgt tccttcccac accaccatca cggagctgcc
    30601 ggaggtcact tcatctccgc gacttaagaa aattgccgaa gggttgctga cttcacgcag
    30661 cccctgttcg gtcatcgcga agacgcccag ctcattcacc gcgccgaagc ggtttttatg
    30721 gctgcgcaag gtgcgaaaac gggagtcggc atcgccatcc aaaagcaccg aacagtcgat
    30781 acagtgttcc agcactttcg ggccagccag cgaaccatct ttggttacgt gccccaccat
    30841 gacaatcgcc acaccgcgcg ttttggcgaa gcgtgtcaaa taagccgccg tttcacgcac
    30901 ctgcgccacg ctaccaggcg atgactgtac atccgccata tgcatcacct ggatcgagtc
    30961 aattaccatc agcttcggtt gctcttcttc ggcaatcagg cagatctgtt cgatgctggt
    31021 ttccgacaac atattgagat tgtcggtcgg caggccaagg cgatgagcgc gcattgccac
    31081 ctgttgcagc gactcttcgc cggtgacata cagcgttttc atctgctgcg ccagtttgca
    31141 cagcgtttgc agcaacagcg tggatttccc ggcacctggg ttaccgccaa tcagaatggc
    31201 gcttcccggc accacaccgc cgcctagtac gcggtcgaac tctttaaatc cggtggaaaa
    31261 acgcggcagc tcttcaaggc tgatatcgga gagtttctgg acttttgcca ccccggcgct
    31321 accggcatag ccgctgagac gctcgttacg cgccaccgtt ggcgacgcag caagacgcac
    31381 ctcggtgatg gtgttccagg catgacaggc actgcactgc ccctgccagc gcggataatc
    31441 ggccccgcat tcattacaaa caaaggcgcg ttttggagct tttgccacgg tgtacctcgt
    31501 taatgctgtg ccgcccgcag gatggcgggc gagcaattac ttctgattca ggctgcctga
    31561 gaggatgcag aataccccca tcaggtcagc gtgacggatg gtgacttccg ccttttcatt
    31621 cacttttggc ttggcatggt aggcaatccc cagccctgcc gctttgatca tcggcaggtc
    31681 attggctcca tcgccaatcg ccacggtctg cgccagcggg atttcatact cctgcgcgag
    31741 gcgagtcaga gttttcgctt tgtactgcgc gtctacgatg tcgccgatca cattgccggt
    31801 aaatttaccg tccatgatct ccagttcatt ggctaccacg gctgtcaggc gcagcttgtc
    31861 gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca atcgccactt tccagcccag
    31921 cgtttccagc ttgagtacca gttgcgttaa gcctggcatc agcggcagat tttcacgcac
    31981 ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc acacggctgc gcaggctggc
    32041 ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact tccgccacca tctcgcccgt
    32101 tccggccagt ttggcaattt catcaataca ttcaatctgg atggcggtgg aatccatgtc
    32161 catcaccagc aaacccggcg tgcgcaggtg cgggattttc cccagcgggg cgacatccag
    32221 ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt gaacctgcca gacgaatcac
    32281 ctgataatct tccacgcacc aggcggcaac aatcaccatc gccgcaccca gtttgctctg
    32341 gtattgggtc agacgctgtt tatccagccc acgaccatac agcagccagc cgctacgacc
    32401 tgcgtggtaa tccagtggca tcacttcatc accacttaat gaaagaggca gacccggcca
    32461 taaagagaca tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg ctcctgtaaa
    32521 atcgttggaa gcagggaaaa taacgcatga ggctaccttg tatccattgc ttctggcaac
    32581 attaagtctc aaattttcaa agggtggaag atggctcgca caaaactgaa attccggctg
    32641 catcgggcag tgattgtcct gttctgtctt gccttgttag tggcactgat gcagggggcg
    32701 tcatggttta gtcaaaacca ccagcgacag cgtaatccac agctggaaga actggcccgc
    32761 accctggcgc ggcaggtgac gctgaacgtt gcaccactga tgcgtaccga ctcaccggat
    32821 gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg aaagccgcat tctcgacgcg
    32881 ggcgtgtatg acgaacaagg cgatcttatc gcccgttctg gcgaaagcgt cgacgtgcgt
    32941 gaccggctgg cgctcgacgg gaaaaaggcg ggcggctatt ttaaccagca gattgtcgaa
    33001 cccattgccg ggaaaaacgg accgctcggt tatctgcgcc tgacgctcga cacccatacg
    33061 ctcgccaccg aagcccaaca ggtggataac accactaaca ttttacgcct gatgttgctg
    33121 ctctcactgg caatcggcgt agtgctgacc cgcacgctgc tacagggtaa acgtacccgc
    33181 tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga ggaagaaagc
    33241 gagaaaaaag agtgacccat tactacaaga aaggaaatcg ttatgtccac attacgcctg
    33301 ctcatctctg actcttacga cccgtggttt aacctggcgg tggaagagtg tatttttcgc
    33361 caaatgcccg ccacgcagcg cgttctgttt ctctggcgca atgccgacac ggtagtaatt
    33421 ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc ggatggaaga agataacgtc
    33481 cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc acgatctcgg caatacctgc
    33541 tttaccttta tggctggcaa gccggagtac gataaaacca tctccacgtc gattgtgctc
    33601 aatgctctga acgcgctcgg agtcagcgcc gaagcgtccg gacgtaacga tctggtggtg
    33661 aaaaccgccg aaggcgaccg caaagtctca ggatcggcct atcgcgaaac caaagatcgt
    33721 ggcttccacc acggcacctt gctgctcaat gccgacctta gccgcctggc aaactatctc
    33781 aatccggata aaaagaaact ggcggcgaaa ggcattacct cagtgcgttc ccgcgtgacc
    33841 aacctcaccg agctgctgcc ggggatcacc catgagcagg tttgcgaggc cataaccgag
    33901 gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa tcatctcccc ggacaaaacg
    33961 ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga gtagctggga atggaacttc
    34021 ggtcaggctc cggcattctc gcatctgctg gatgaacgct ttacctgggg cggcgtggaa
    34081 ctgcatttcg acgttgaaaa aggccatatc acccgcgccc aggtgtttac cgacagcctc
    34141 aaccccgcgc cgctggaagc cctcgccgga cgactgcaag gctgcctgta ccgcgcagat
    34201 atgctgcaac aggagtgcga agcgctgttg gttgacttcc cggaacagga aaaagagcta
    34261 cgggagttgt cgacgtggat agcgggggcg gtaaggtaac tcgctgatta aaatatccaa
    34321 ggaaggaaat atgatcgatc tggaaaacca ggaacgggag attatcaatt taatgttcag
    34381 ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt
    34441 aagtaaaatg ttaggtatct cgattaattc tctaaaacag atcgaaaaga cggaacggct
    34501 tagctcaaac atcaaaagca aaatggcaga aatttatggt tgtccaccag agttgctgat
    34561 atgcccttcc tggatgacgg ctgaacataa atgaatagat tcgtgaatag atacttgaat
    34621 gtattttatt ttctatccat atgaaaaata atagtaaatc tataaatcag ttgctattga
    34681 tgaatagata ctaaaaagcc ggagcagtct cccgctccgg cttaacacaa caatcgcctt
    34741 tcagcgaaac acaattactc tttatcgccc agcagaacgg attccagtgc gattttgatc
    34801 atgtcgttga aggtggtctg acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg
    34861 tcagatacgg tgcagatggt cagggctttc gcgccaaatt ctgcagcgac gccgtagata
    34921 ccagccgctt ccatttccac gccgagaatg ccgtattttt ccatcacgtc gaacatttcg
    34981 ccgtccggag agtagaacag gtcagcggag aacaggttac caacgcgagc atcaatgccc
    35041 agtgctttag ctgcatctac tgcgttacgc accatgtcga agtcagcgat agcggcaaag
    35101 tcatggtctt taaaacggat gcggttaact ttggaatcgg tgcaggcacc cataccgata
    35161 acgacgtcgc gcagttttac gtgcggcaga actgcgccac aggaacccac gcggataatt
    35221 ttcttcacgc cgaaatcggt aatcagttct ttggtgtaga tggagcagga cgggataccc
    35281 ataccgtgac ccattacgga aattttgcgg cctttgtaag taccggtgaa gcccagcata
    35341 ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag tttcagcaat atacttcgca
    35401 cgcagcgggt cgcctggcat caaaactacg tcagcgaaat cgcccatttc tgcattaatg
    35461 tgtggggtag ccattgtttt atccttatca acatgttttc accctctccc tgtgggagag
    35521 ggccggggtg agggcatcaa gccgcaccca ttatcagaac atggctttgc catattccat
    35581 atcagaagta ccaaaatatt ttgccagagt ctggccgata tccgcgaagg tttcacggtg
    35641 acccagtgag cccggtttta ctttcgggcc gtaaaccagt accggaatgt gttcacgcgt
    35701 gtggtcagta ccggtccagg tcggatcgca accgtggtca gcggtgagga tcaggatgtc
    35761 gtcatcgcgc agcagagaca tcagctccgg cagacggcgg tcgaacagtt ccagacccgc
    35821 ggcataaccg gcgacgtcgc gacggtggcc ccaggaagag tcgaagtcaa cgaagttggt
    35881 gaagacgatg gtgttatcac ccgcttcttt catctctttg atggtggcgt caaacagcgc
    35941 gtccaagcca gtcgctttca cttttttggt gataccgcag ttggcgtaga tgtccgcaat
    36001 tttaccgaca gaaaccacct ggccgtgttt ttcatcaacc agtttctgca gcacggtcgg
    36061 tgctggcggc tcaacagcca ggtcgtgacg gttaccggta cgctggaagt taccggcttt
    36121 gtcgccgata aacggacgag cgataacacg accgatattg tagccgccgt tggtcagctc
    36181 ttcacgggcg atttcgcaca gttcgtagag tttatccaga ccgaaagttt cttcatggca
    36241 ggcaatctgg aacacggagt cagcggaggt atagaaaatc ggcttgccgg ttttcatgtg
    36301 ctcttcgccc agctgatcca gaatgaccgt accagaagag tggcagttac cgaggtaacc
    36361 cggcagatta gcgcgttcaa ccagtttatc cagcagctct tgcgggaagc tgttttcgtg
    36421 atcggagaaa tatccccact caaacagaac cgggacaccg gcgatttccc agtgaccaga
    36481 cggggtatct ttaccggatg acatttcgtg cgcccatgcg tacgcgccga taacttcagc
    36541 gttgccgtcc attcccgccg gaatgaaacc ggtagaacct tcgtgtgctt tcgccagccc
    36601 cagacgggtc agatttggca gattgagcgg gcctttacga ccgttatcag cttcgccttt
    36661 ggcacaagct tctgcgatat gacccagggt gtcagccccg acgtcaccaa agcgttctgc
    36721 atcttctgta gcgccgatgc cgaatgagtc cagcaccata ataaatgcac gtttcatatg
    36781 ttctccgtac tcagtgcttc aaatttaaat gatcagatca gtataccgtt attcgctgat
    36841 acggcgatag acagttggtg tgctttccgg tgctttatcg gcaagtttaa ttgccgcttt
    36901 caccgctttc gccgcttcct gccagctgtt ttcgtctttc gcgtggataa ccgccagcgg
    36961 acgctgaccg tctacctggt cgcccagacg cgccatatca gtaaagccga cgctgtaatc
    37021 gatggtgtca gatgcctgac gacgaccgcc gcccattgca accactgcca tccccagcgc
    37081 gcgggtatcc atttcactga caaaaccttc ggtatcagca tagactgctt tcgtcagcat
    37141 cgctgtcggc agatacttcg cgtagttctc aacgaagtcg gttgggcctt tttgtgccgc
    37201 taccatacga ccaaagactt ctgccgcttt accgttgtcc agcaccgcct gcaatttcgc
    37261 gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc agcatctcca cacacagcgc
    37321 catcgtgaca tcaaacagac gcgggttacg atattcaccc gtcaggaact gcaccgcttc
    37381 acgaacttca accgcgttac ctgcactgga ggccagtacc tgattcatgt cggtgagcag
    37441 cgcggtggtg cgcacgccag cgccgttagc cacgccaaca atcgcttcgg caagggcttc
    37501 agagagttcg taggtcggca taaatgcgcc gctacccact ttcacgtcca tcaccagcgc
    37561 gtccagacct tccgccagtt tcttcgccag aatagaggca gtgatcagcg ggatggagtc
    37621 cacggttgcg gtaatatcac gggtcgcgta gaaacgttta tccgccggag ccagtgaact
    37681 ggtctgaccg ataatcgcca cgccgacgtc tttaataatt tcgcggaaac ggttgtcatc
    37741 cgggaaaatg tcgaagccag ggatggattc cagtttgtcg agcgtaccgc cagtatgacc
    37801 gaggccgcga ccggagatca tcggaatata gccgccgcag gctgcgacca tcggccccaa
    37861 catcagcgaa gtcacatcgc cgacgccacc ggtggagtgt ttatcaacaa tcgggccatt
    37921 cagatgcagg cttttccagt cgagaacggt tcctgaatct cgcatcgcca tggtcagcga
    37981 gacacgctca ggcattgtca tatcgtggaa gaaaatggtc atcgcgaggg cggcaatctg
    38041 cccttcggag atagtgttgt cgcgaatacc gttgataaag aaacgaattt cttcatcgct
    38101 cagcgcatga ccatcacgtt ttttacgaat aatttcttgt gcgagaaaca aggtaacctc
    38161 ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc tacgccttat ccggcctacg
    38221 ggtagatacg tctcgtaggc ctgataagcg cagcgcatca ggcgtaaagc atcttactta
    38281 gtagctgctg gcgctcttac cgtcgccgtg acccagcgct ttcagcaggc ttgccagcag
    38341 gctggaagca ccaaagcggt agtgacgcgc atcagcccag tcagcaccga acagttcatc
    38401 tgcgatggcg agatatttct gcgcatcttc cgcagtacgc acgccgcccg ccggtttgaa
    38461 accaacggtt ttttctacgc ccatatcacg gatcacttcc atcatgatgc gcgcgctttc
    38521 cggcgtcgcg ttcacagcca ctttaccggt agaggttttg atgaagtccg cacccgcttt
    38581 gatggagatt tcagacgctt tacggatcag cgcttcgtct ttcagttcgc cggtttcgat
    38641 gatcactttc agcagtacat tcgctgccgc gcaagcctct ttacaggctt tcaccaggtc
    38701 aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac gggaacacaa cgtcaacttc
    38761 atcagcaccg taggcgattg ccgcacgggt ttctgccagc gcgatttcga tgtcgtcgtt
    38821 accgtgtggg aagttggtta ccgtagcaat acggatttcc ggggtgccct gctctttcag
    38881 tgttttgcga gcaatcggga taaagcgagg atagatacag atagcggcgg tattgccgac
    38941 cggggttttg gcctgatgac acagagcaat tactttctcg tcggtgtcgt cgtcattcag
    39001 ggtggtcagg tccatcaatt tcagtgcacg caggctgctt gctttcagat cagtcatttc
    39061 attctcctgg cttgtcgcca ataaaattca ccttgcgagt ttgttagtat tctaacatct
    39121 actccgcaac acacttcgat atacatcaca attaaggaaa tctacttaca agtttgcatc
    39181 actataatgc gatctggttc aaataattca ctttcaaatg aatgcgtcag tggtggcaaa
    39241 cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa tggcgcatca ccttcggcat
    39301 aaaaggaaaa gaagatgccc acttctcatg aaaatgcact gcaacaacgt tgccagcaaa
    39361 ttgtcaccag cccagtgctt agcccggagc agaagcgcca ttttctggca ctggaagcag
    39421 aaaacaatct gccttaccca cagcttcctg ccgaagcccg ccgcgcgctg gatgaaggtg
    39481 taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc gcgctatgtc ttacccgatt
    39541 acgcccgttt tctggcgaac ggttccgaat ggctggagct ggaaggcgcg aaagatcttg
    39601 atgacgcact ctctctgctg accattcttt accaccacgt accgtcggtc acatcgatgc
    39661 cggtctacct ggggcaactg gatgcgttgt tgcaaccgta tgttagaatt ctaacacaag
    39721 acgagatcga tgttcgaata aaacgtttct ggcgttacct cgacagaacc ctgccagacg
    39781 cctttatgca cgccaatatc ggcccgtctg attcgcccat tacccgcgcg atcttacgtg
    39841 cagacgcaga gttgaagcag gtttcaccga acctgacctt tatctacgat cctgaaatca
    39901 cccctgatga cctgctgctg gaagtggcga agaacatctg tgaatgtagc aaaccgcaca
    39961 tcgccaacgg tccggtgcat gataaaattt tcacaaaagg gtgctacggg attgtgagct
    40021 gttacaactc actgccgctg gcgggtggtg gcagcacgct ggtacgcctt aacctgaaag
    40081 ccattgccga gcgcagcgaa tcgctggatg acttctttac gcgcactcta ccgcactact
    40141 gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt cctctatcaa caatcacact
    40201 tctttgagaa tagcttcctg gtgaaagaag ggctgattaa ccctgaacgt tttgtgccaa
    40261 tgtttggcat gtatgggctg gcggaagcgg ttaacttgct gtgtgaaaaa gaagggattg
    40321 ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtagg ttatcgcatc agcgcgcaac
    40381 tggcggagtt tgtcgccaat acccccgtga aatatggctg gcaaaaacgc gccatgttac
    40441 acgcacagtc ggggatcagt tccgatatcg gcaccacgcc gggcgcgcgt ttgccgtatg
    40501 gcgatgagcc agatccgatc acccatctgc aaactgtcgc cccgcatcat gcttattatt
    40561 attccggcat cagcgacatt ctgacgctcg acgaaaccat caaacgtaac ccgcaggcac
    40621 tggtacagct ttgcctcggt gcctttaaag ccggaatgcg tgaatttacc gccaatgtca
    40681 gcggtaacga tctggttcgc gttaccggtt atatggtgcg tttgtcggat ttagaaaaat
    40741 atcgcgccga aggttcacgt accaacacca cctggctggg cgaagaagcc gcacgcaaca
    40801 ctcgtattct ggaacgccag ccgcgcgtga taagccatga acagcagatg cgctttagtc
    40861 agtaagatta tccccttctc ctgcgttgac gggccaggca gtcgtctggc tctgtttttg
    40921 cagggctgca atctgcgctg caaaaactgt cacaatccgt ggacgatggg acgttgcaat
    40981 gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt tgcagattgt tgacggcaaa
    41041 gtggtgtgga acgctgtggt ctgcgagcaa tgtgatacct gcctgaagag gtgtccgcaa
    41101 catgccacgc caatggcgca atccatgagc gtggacgaag tgcttagcca tgtccgcaaa
    41161 gcagtgctgt ttatcgaagg gataacggta agcggcggcg aagccacgac ccaactgccg
    41221 tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc aactgcgcca cctcacctgc
    41281 ctggtagaca gtaacggtat gttgagcgaa accggctggg aaaaattgct cccggtgtgc
    41341 gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat gtcatcaaca actcaccgga
    41401 cgcgataatc agcagattaa gcgcagcatc tgtttgctgg cagagcgcgg taagctggcg
    41461 gaactgcgtt tgctggtaat ccctggccag gtggattatt tgcaacacat cgaagaactg
    41521 gcggcgttta tcaagggagt cggcgatgtt ccggtacgcc tgaacgcgtt tcatgcccac
    41581 ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc cggaagacgt tgagccgttg
    41641 gctgatgcat taaaggtgcg cggggtgagc cggttgatat ttccggcgct ctatttgtga
    41701 catgccggat gcggcgtaaa cgccttgtcc ggcctacggc acgttaaaca acgtatacgt
    41761 attattaagc aacacttcgg caatctcatc cgcgggttcc gggcgtaact cgcaaagcac
    41821 ggcaaacaca cgggcagcat gctccgggcg gttaggctgc ccctgaaaac cgttgagcgg
    41881 catatccggc gcatcggttt ccagcaacaa cgatgccagc ggtaattttg cgatgacatc
    41941 gcgggtttta ctggcgcgtg gataggtgat agtaccgcct acgccgattt tgtagcccag
    42001 ctgtacaaac cgttcagcct gttgctggct gccagaaaat ccgtggacaa caccagcacg
    42061 cggcaaatcg tggcgtttaa gatgcatcac cagtttgtcg tgcgtgcgcc gtgaatgcag
    42121 gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta accactgctg
    42181 cctctcaaat tgcggatcgt cgccgaagag atccagaccg atctccccca ccgccaccac
    42241 cttcgccgga cgcctttcca gcgcctgctg tagctgatca agagacacat cgctatgttt
    42301 ttccaacata ccaggatgca agcccaatgc ggcatacagc ggttgataat tttccgctaa
    42361 cgcctgcacg cgggcaaaat tttccgcctc ggttgccgga acaatgatct tgcctacgcc
    42421 cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa
    42481 atggcagtgg gtgtcgataa aacggcaaat caagccagat cctcattgtt aaatgtcggg
    42541 tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg tatcgttggc gactggcgca
    42601 ggcggaacga caatcgattc cggcgtcacc actggcacca gatggcgggt aagcggcgct
    42661 ttttcagtta atagcttgcc aaccgtggcg aggaaataac gaccgcataa acgcccaagt
    42721 ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg taatggcttc
    42781 ggcggataaa tttcgaatat ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg
    42841 tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag gctactgtca
    42901 cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg tgacggcaca
    42961 gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac cggaatcgca
    43021 tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct acgataaaag
    43081 ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt tggtaaaaag
    43141 taattcggcg cgtagtcatc ctgacgacag gcgcacatat aaaacgattt gccgctgtca
    43201 aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc ctccaccagc
    43261 cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa aaattcgcgt
    43321 tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca gataaacgcc
    43381 gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt aaactgcgcg
    43441 cgcataaact catccagcac gccagccgtg aaaattccac gctgcccgcc gccttcgcac
    43501 accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat attaccaagc
    43561 gttacaggta ttcgctgccc cacctctgcc ttcctgtttt tgttgtgaaa gaatgacaaa
    43621 gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact ggctttggat
    43681 aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca aactcaccag
    43741 gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg taccggccag
    43801 accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga tgccccaacg
    43861 aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca
    43921 ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg
    43981 aattacgacg aattacttag ttttcagatc atttttaacg cttttcacac catctaccgc
    44041 tttggcgata ctttcagcac ggtcactttg tgcctgagaa tcgacggtac cggagagctg
    44101 aaccacgccg tcggtggttt caactttcac atgacgggaa gggacgatat cgtccgccag
    44161 cagtttggct ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct tcaccgagcc
    44221 ttctttagcg tcgcgaacgt gcagtttgtc gctgacagag gtcacccctt caacgccttt
    44281 cgccactttc actgcctctt cggcctgggc ctggctttca acgaaaccgc tcagggtcac
    44341 gactttttga tcggttttta cagagatatc ggtgctcttg atgttgtcat gatccaccag
    44401 ggccgccttc actttcgcgg tgatggcgct gtcatccatg aaattaccga ctttattcat
    44461 agagctatcg actttttgcc ctgcgctttc attggtagtc tgcgcgttgt tttccgcgta
    44521 ggcagagccg gtcgcgacgg cagaggtcaa cattacagcc agcagagttt tcgaaatctt
    44581 cagtcttgtc atagtcatcg atttattcct gtatgtttgc tcgtaatttg agcctggcaa
    44641 cacgaggttg cattgctgaa tagggagaga cttcaccctc tacagaagtc aatggtcgcc
    44701 atcacaaaag cgatgagtga tgaataacga ccattacagc ctctgaatca gttattaata
    44761 tcggtagaat gacaatcgac ggctttagat actgatatct acgcattgaa cggtatttaa
    44821 cgccgtcaga aatgtcatca ctttgttaaa tatagatcac aattttgaaa ccgctcggga
    44881 tatcagcgaa aacataagca aaagtgaatg ttttaagaac attccgtaag cggctaataa
    44941 ggaagggaaa ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa ttaatgctcg
    45001 cgggtctggt ggaactgaac gtccggataa cgttcctgtg ccaggcgcag gttgaccatg
    45061 ctggtagcga tgtaagcgag gttatcgccg ccatcaagcg ccagttggct ttcgttctta
    45121 cgcttgaact cttcgaattt cttcgcgtct gcacattcta cccagcgggc agtggcaacg
    45181 ttgactgact catacactgc ttcaacgttg tattcgctct tcaggcgcgc taccaccaca
    45241 tcaaactgca gcacaccaac tgcaccaacg atcagatcgt tgttggagat tggacggaac
    45301 acctgcaccg cgccctcttc ggaaagctgt accagccctt tgagcagctg tttttgcttc
    45361 agcggatctt tcaggcggat acgacggaac agttctggtg cgaagttcgg aataccggtg
    45421 aacttcatca tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc gtggttgtgc
    45481 aggccgagga tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc cgccataaag
    45541 gtcagcgcgt cggagatcac cacatctttc gcagtgcgca cctggcgcag tttcatgcct
    45601 ttttcatatt taccggacac cacacgcata aacgccacgc ggtcgcggtg tttcgggtcc
    45661 atgttggcct gaattttaaa tacgaagccg gtaaatttat cttcgctcgc ttctacggta
    45721 cgggtatcgg tctgacgcgg catcggcgca ggcgcccact ccaccaggcc atccagcata
    45781 tgatcaacgc cgaagttacc cagcgcagta ccgaagaata ccggagtgat ttcgcccgca
    45841 aggaacagct ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag ttcgtcacgc
    45901 agctgctgtg ccagatcttc accaaccgca gcatcgagat ccgggttatt cagcccttta
    45961 acaatgcgga cttcctgaat ggtgtggcct ttaccgctct gatagagata ggtttcatct
    46021 ttataaaggt ggtaaacgcc tttaaacagc ttgccgcagc caattggcca ggtgatcggt
    46081 gcgcagccag ttttcagctc gttctcaact tcatcgagca attccatcgg gtcgcggata
    46141 tcacggtcaa gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg ggtaacttcc
    46201 atcagcttac gggtacgatc ttcaacacct tttgcggcgt cgataaccat caggcagcag
    46261 tccaccgccg tcagggtacg ataggtatct tccgagaagt cttcgtgccc cggggtgtcg
    46321 agcaggttaa ccaggcaatc gtgatacgga aactgcatca cagacgtagt aatggagatc
    46381 ccacgctgct tttccatctc catccagtcc gacttagcgt gctggttgga accacggcct
    46441 tttactgtac cggcggtctg aatggcctgt ccgaacagca gcaccttctc ggtgatggta
    46501 gtcttaccgg cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc cacctcttgc
    46561 aaataaggag acaacgtcat aattctttct tcttgagtaa gcggcatcgc gccgcgcttg
    46621 ttgaaaacga aaaattgcgg ctattttacc catcaacggg ggggaggcaa tcagtgttta
    46681 cacaggagct gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc gatgccttct
    46741 ggttgctcgc gatggtgtgc attcagccag caggtcgcaa gcccggcgtt gatgccaccg
    46801 agaatatcgg actcggcagt gtcgccaacc atcagcacgc gtgaacggtc aggattgccc
    46861 gcctgttcca gcgcataatc gaaaattttc ttattcggtt tggcaacgcc aacttcttcg
    46921 gaaatcacca gcaaatcgaa gtaatcacgc aggcccgtgc gttccagacg cacctgttgc
    46981 aaggcactaa agccgttggt gatgatgccg attttggcgt tgccacgaat ggcgttaagc
    47041 agagaaaccg cgcccggcag cggcgtgcag atttccgcca tcgcattaat aaaggcttcg
    47101 ttgagtttac ctggctcgac gttcagccgt tcggcccagc tctcaaaccg cccgtgctga
    47161 agctgtaatg aagtgatcgc gccgttttga taatccaccc acagtggctt gttaacggcc
    47221 tgatagtcct gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa catccgctgc
    47281 aggccggtga atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa aatccagtcc
    47341 cacttcatta ttccaccttg tcttacatac tgattggcaa cgccatgatg atggcgtctt
    47401 cgcgaccgtc cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta aagcctaaac
    47461 tttcgtacag ggcaatggcg gcagcgtttg aagcacggac ttccagccag agcgtcgcca
    47521 cgccgcgttt ttccagttcg tcgatcagat gttccagcag cgcccttccc aatccctgac
    47581 gctgatagtc aggatcgacc gcaatattga acaatgtagc ttcatccagc accacttgcg
    47641 taatcgcaaa cgccgccatt ttgccgtttt gcgttaactg aaagttgaga taacgctcgc
    47701 cctggttgct ggcaaagttt ttttcactcc acggaaaggc gtgggcgcgt tgttcaatgt
    47761 ggtaagccgc tggtaaatca gtcgtttcga gggaagaaat cgtgttcata tgtgcaaatt
    47821 tgttgccata acgcggcgcg tgccgttggg tttgcccgta attcggtgag cgccggtgat
    47881 gccacctgag cgccttccag tgatagcggt tcgtcagtac ccaaccgcca gctgttgcag
    47941 cgactgcctt gcggcagcat cgcgattttt tctggcgtca gttgcagcac ttgatcgggg
    48001 ctgacggtta atgcacgcag aacatcgctc actaaaggat cagtcagggc gggaagatcg
    48061 tttgccacca tcaccagacg gacgtgtgcc gggatcgcaa tggcaatctc cccctgcaac
    48121 gcgccagggc gacgcagcga ccactgggta atgcccagtt gctgtaactg ccagtctcgt
    48181 cgggatgtca tagcgaatcg ctcctgttgt caggggcgca aatatagcaa attcgtcgat
    48241 accgcgccaa catatggcta taatcgccgc cagtatcaat tgaggagcat tccatgtctg
    48301 catttacccc ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa caaagccgta
    48361 ttctgtttgc cggagactta caggatgacc tgcccgcgcg tttagatacc gcggccagcc
    48421 gtgctcatac ccagcaattc caccactggc aggtgttaag ccgtcagatg ggggataacg
    48481 cccgttttag tctggtcgct acggcggatg acgtcgcaga ttgcgatact ttgatttact
    48541 actggccgaa gaacaaaccg gaagcccagt tccagttgat gaatttactt tctctgctgc
    48601 cggtggggac ggatattttt gttgttggcg agaaccgcag cggcgtgcgc agcgccgagc
    48661 agatgctggc tgattatgcg ccgttgaata aagtcgacag cgcccgtcgc tgtggcctct
    48721 attttggtcg tctggaaaaa cagccggtat ttgatgccga taaattctgg ggcgaataca
    48781 gcgtcgatgg cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac ggtctggatg
    48841 tcggtagcca gttgctgctc tcgacgttaa ccccgcacac gaaaggtaaa gtgctggatg
    48901 tcggctgtgg cgcgggcgtg ctttcggttg ccttttcgcg ccattcgccg aaaattcgtc
    48961 tcactctgtg cgatgtctct gcgccagcgg tagaagccag ccgcgcaaca cttgcggcca
    49021 acggtgttga aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa ggtcgttttg
    49081 atatgatcat ctccaacccg ccgttccacg atgggatgca aaccagcctg gatgcggcgc
    49141 aaacgctgat tcgcggcgcg gtgcgtcatc ttaatagcgg cggcgagctg cgaattgtag
    49201 cgaacgcctt cctgccttac ccggacgtgc tggatgagac atttggcttc catgaagtga
    49261 ttgcgcaaac cgggcgcttc aaggtgtatc gcgccattat gacccgccag gcgaagaaag
    49321 gttaattatc tcgccgaata ccgtgtcgga tgcggcgtac acgtcttatc tgaccgacaa
    49381 aacctgtacg ttagcccttc gtaggccaga taagacgcgc cagcgtcgca tctggcatct
    49441 gcaccccaat accgtgtcgg atgcggcgtg aacgccttat ccgacttaca aaacctgcac
    49501 gttagtctcc tgatggacat ttttccagca attacacctc tgtcgataat taactattga
    49561 cgaaaagctg aaaaccacta gaatgcgcct ccgtggtagc aattcttttt aagaattgat
    49621 ggtatgcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gtccttacgg
    49681 acgtgggggt tcaagtcccc cccctcgcac caattatctt tacttccttt cttgtttctt
    49741 ccctgttttg tcatccctga ttcaaattca ttatcatcag catgatgcca gcaataagcg
    49801 ccaggcacga tggtagcaaa atgtaatgcc gcaactgttt gtgatacagc atcaccggcg
    49861 tcaatactaa tcccatcaga atgacaatcc gccactgatc cggcgataaa tccgcataca
    49921 tcagccctgc aatcagcaat cccggcagca acactcccca gccactgccc agcgtacgtt
    49981 gcaacatggt ttcatctctt tcattgataa tgataaccaa tatcatatga taattcttat
    50041 catttgcaag ccagagaaat cccttgctat cgggtaaacc tatcgctagg attagcaatc
    50101 attatcattt agattactat cccgattatg gcctatcgtt ccgcaccgct ctatgaagat
    50161 gtcatctggc gaacacatct ccagccgcag gatgctgggc ttgcacaagc cgtacgggcg
    50221 atgatcgcca aacatcgtga acatttgctc gagtttatcc gcctggatga acccgctccg
    50281 cttaacgcca tgacgctggc gcaatggtca tcaccgaatg cgctaagttc tctactggcg
    50341 gtctattccg atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa caaaccgctg
    50401 atctcactat gggcacaatg gtatatcggc ctgatggtgc caccattaat gctggcgcta
    50461 ctgacgcagg aaaaagcatt agatgtgtcg ccggaacatt tccatgctga gtttcacgaa
    50521 accggacgcg tcgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc aacaccacat
    50581 tcgccgcagc agcgaatgga aacgttaatc agccaggcgc tggttccggt tgtgcaagca
    50641 ctagaagcga ccggagagat caacggcaaa cttatctgga gtaataccgg ttatttgatc
    50701 aactggtatc tcactgagat gaagcaactg ctcggcgagg cgactgttga atcgctgcgc
    50761 catgccctct tttttgagaa aacgctcacg aacggtgaag ataatccact ctggcgtacc
    50821 gtggtactgc gcgacggcct gctggtgcgc cgcacttgct gccagcgtta tcgcttaccg
    50881 gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc actgccgggg aagtaaaccc
    50941 ggcatcatgc ggattaatag ggatgcaaca cattacttgt ttcgcaatgc cgcatacata
    51001 acagaatatc tgcggcctcc aacaaccctg cgtcagaact gacgcccagt ttcgacatca
    51061 cattaaattt atgtgcacgg atcgttttga tattgcgttt aagctgctcg gcaatttgtg
    51121 tcattgagta gccacgcgac ataaagcgca atatttctct ctccgtcggg cttaacgtcc
    51181 ggctttggtt aatgtaccac tgattgttca gtcggtcggt cgcctgacgt acaccattta
    51241 atgacaaaaa gagttcctga tgaaaaatct ccagcgttga cgctttactt aatacaccgt
    51301 ccagcggtga tggcgacaat gaaccaatca gccgagcttc aatatcatca tccgcaataa
    51361 ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt caggcaagat aatccctctc
    51421 tgcgctcact tctcatggca gaaaaagaaa aaatgaccgc cgcaaacgag atccgcgaca
    51481 tggcatcctg aaatgactcc tgactggtat aggtatggag cttataaggg ttgcccgcaa
    51541 actttctgaa taaactctca ataccgatac tactcatgac gcatttttct atgagtgcga
    51601 catttctctt tttaattcgg ctgtgttcca ttctgcggca tctccataaa cagaaggcaa
    51661 gttaagttct cttgcaccct gcgcgctatt tatccatgca tacatttcag cgttgctgtg
    51721 aattgccagt cgacgcatcg cactgttttt ttgggcgctg atagttttat tacttttctt
    51781 aagcaatgaa gctatctggt tgatgcccca tcctttacct aatagccgca gtaccttgcg
    51841 ctctgaaaga gtgagaatga cggagcgata gctaaaatca tgagtttcag tcattgccgg
    51901 ggaggtcagc atggcgctga tacgctctgc gtgcgtattg ccggaacgta cggtcttgac
    51961 cagattctca atgggttcaa catcagacaa taacgtcgcc gtagggcaca tgagcagttc
    52021 tactgcctgg gaataccagg agcgtgacac catgaaaacc cagtgaattt cccgatattg
    52081 tgagattaaa gaataataat gttcacaaac agaacgtgga tcttcacttt caccggctaa
    52141 atcagcaatg actaatccgg aacgacgtaa ttgtaataag gtaaggtcct ccgcagaggc
    52201 gctggatatt atttcatatt cagggaagtg agtcctcatg acctctttta accctgcttg
    52261 cataacaggt attttactga taacaattcc atttttgcag catcctggca acatatttcc
    52321 tccacatcca ctgtctaaaa attatgatgg tttattcagg atattca
//
LOCUS       DAPHJE010000014        51283 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPHJE010000014 DAPHJE010000000
VERSION     DAPHJE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.014"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            24..725
                     /locus_tag="RH222_000876"
     CDS             24..725
                     /locus_tag="RH222_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3795935.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            750..3350
                     /locus_tag="RH222_000877"
     CDS             750..3350
                     /locus_tag="RH222_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV3795936.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVCDDYTAGDFFDSVSFRGVQLASDDDM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3341..4411
                     /locus_tag="RH222_000878"
     CDS             3341..4411
                     /locus_tag="RH222_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3795937.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4423..4965
                     /locus_tag="RH222_000879"
     CDS             4423..4965
                     /locus_tag="RH222_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3795938.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4973..5488
                     /locus_tag="RH222_000880"
     CDS             4973..5488
                     /locus_tag="RH222_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3795939.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5454..6191
                     /locus_tag="RH222_000881"
     CDS             5454..6191
                     /locus_tag="RH222_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3795940.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6302..7312
                     /gene="pyrD"
                     /locus_tag="RH222_000882"
     CDS             6302..7312
                     /gene="pyrD"
                     /locus_tag="RH222_000882"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV3795941.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7486..8028
                     /gene="zapC"
                     /locus_tag="RH222_000883"
     CDS             7486..8028
                     /gene="zapC"
                     /locus_tag="RH222_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV3795942.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8025..9134)
                     /gene="ycbX"
                     /locus_tag="RH222_000884"
     CDS             complement(8025..9134)
                     /gene="ycbX"
                     /locus_tag="RH222_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV3795943.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9378..11486
                     /gene="rlmKL"
                     /locus_tag="RH222_000885"
     CDS             9378..11486
                     /gene="rlmKL"
                     /locus_tag="RH222_000885"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV3795944.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11498..13405
                     /locus_tag="RH222_000886"
     CDS             11498..13405
                     /locus_tag="RH222_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3795945.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAVPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13535..14788
                     /gene="pqiA"
                     /locus_tag="RH222_000887"
     CDS             13535..14788
                     /gene="pqiA"
                     /locus_tag="RH222_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV3795946.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14793..16433
                     /gene="pqiB"
                     /locus_tag="RH222_000888"
     CDS             14793..16433
                     /gene="pqiB"
                     /locus_tag="RH222_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV3795947.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16430..16993
                     /gene="pqiC"
                     /locus_tag="RH222_000889"
     CDS             16430..16993
                     /gene="pqiC"
                     /locus_tag="RH222_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV3795948.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17249..17416
                     /gene="rmf"
                     /locus_tag="RH222_000890"
     CDS             17249..17416
                     /gene="rmf"
                     /locus_tag="RH222_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV3795949.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17486..18004)
                     /gene="fabA"
                     /locus_tag="RH222_000891"
     CDS             complement(17486..18004)
                     /gene="fabA"
                     /locus_tag="RH222_000891"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV3795950.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18073..19833)
                     /locus_tag="RH222_000892"
     CDS             complement(18073..19833)
                     /locus_tag="RH222_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV3795951.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20019..20471
                     /gene="matP"
                     /locus_tag="RH222_000893"
     CDS             20019..20471
                     /gene="matP"
                     /locus_tag="RH222_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV3795952.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20547..21599)
                     /gene="ompA"
                     /locus_tag="RH222_000894"
     CDS             complement(20547..21599)
                     /gene="ompA"
                     /locus_tag="RH222_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV3795953.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(21956..22465)
                     /gene="sulA"
                     /locus_tag="RH222_000895"
     CDS             complement(21956..22465)
                     /gene="sulA"
                     /locus_tag="RH222_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV3795954.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22684..23313
                     /gene="sxy"
                     /locus_tag="RH222_000896"
     CDS             22684..23313
                     /gene="sxy"
                     /locus_tag="RH222_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV3795955.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23276..25438)
                     /gene="yccS"
                     /locus_tag="RH222_000897"
     CDS             complement(23276..25438)
                     /gene="yccS"
                     /locus_tag="RH222_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV3795956.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGIHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25448..25894)
                     /locus_tag="RH222_000898"
     CDS             complement(25448..25894)
                     /locus_tag="RH222_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV3795957.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26017..28071
                     /gene="helD"
                     /locus_tag="RH222_000899"
     CDS             26017..28071
                     /gene="helD"
                     /locus_tag="RH222_000899"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV3795958.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPARLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28103..28561)
                     /gene="mgsA"
                     /locus_tag="RH222_000900"
     CDS             complement(28103..28561)
                     /gene="mgsA"
                     /locus_tag="RH222_000900"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV3795959.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28657..29319)
                     /locus_tag="RH222_000901"
     CDS             complement(28657..29319)
                     /locus_tag="RH222_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV3795960.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29492..29905
                     /locus_tag="RH222_000902"
     CDS             29492..29905
                     /locus_tag="RH222_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV3795961.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29950..30267)
                     /gene="hspQ"
                     /locus_tag="RH222_000903"
     CDS             complement(29950..30267)
                     /gene="hspQ"
                     /locus_tag="RH222_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV3795962.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30325..31515)
                     /gene="rlmI"
                     /locus_tag="RH222_000904"
     CDS             complement(30325..31515)
                     /gene="rlmI"
                     /locus_tag="RH222_000904"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV3795963.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31610..31888
                     /gene="yccX"
                     /locus_tag="RH222_000905"
     CDS             31610..31888
                     /gene="yccX"
                     /locus_tag="RH222_000905"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV3795964.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(31885..32214)
                     /gene="tusE"
                     /locus_tag="RH222_000906"
     CDS             complement(31885..32214)
                     /gene="tusE"
                     /locus_tag="RH222_000906"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV3795965.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32305..32964)
                     /gene="yccA"
                     /locus_tag="RH222_000907"
     CDS             complement(32305..32964)
                     /gene="yccA"
                     /locus_tag="RH222_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV3795966.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33171..33258)
                     /locus_tag="RH222_000908"
     tRNA            complement(33171..33258)
                     /locus_tag="RH222_000908"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33222..33224),aa:Ser,seq:tga)
     gene            33685..34803
                     /gene="hyaA"
                     /locus_tag="RH222_000909"
     CDS             33685..34803
                     /gene="hyaA"
                     /locus_tag="RH222_000909"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV3795967.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGNQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34800..36593
                     /gene="hyaB"
                     /locus_tag="RH222_000910"
     CDS             34800..36593
                     /gene="hyaB"
                     /locus_tag="RH222_000910"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV3795968.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            36612..37319
                     /gene="hyaC"
                     /locus_tag="RH222_000911"
     CDS             36612..37319
                     /gene="hyaC"
                     /locus_tag="RH222_000911"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV3795969.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            37316..37903
                     /locus_tag="RH222_000912"
     CDS             37316..37903
                     /locus_tag="RH222_000912"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV3795970.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37900..38298
                     /gene="hyaE"
                     /locus_tag="RH222_000913"
     CDS             37900..38298
                     /gene="hyaE"
                     /locus_tag="RH222_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV3795971.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            38295..39152
                     /locus_tag="RH222_000914"
     CDS             38295..39152
                     /locus_tag="RH222_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV3795972.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39286..40830
                     /gene="appC"
                     /locus_tag="RH222_000915"
     CDS             39286..40830
                     /gene="appC"
                     /locus_tag="RH222_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV3795973.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40842..41978
                     /gene="appB"
                     /locus_tag="RH222_000916"
     CDS             40842..41978
                     /gene="appB"
                     /locus_tag="RH222_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV3795974.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41991..42083
                     /gene="cbdX"
                     /locus_tag="RH222_000917"
     CDS             41991..42083
                     /gene="cbdX"
                     /locus_tag="RH222_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV3795975.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42163..43461
                     /gene="appA"
                     /locus_tag="RH222_000918"
     CDS             42163..43461
                     /gene="appA"
                     /locus_tag="RH222_000918"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV3795976.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKMSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43581..45761)
                     /gene="etk"
                     /locus_tag="RH222_000919"
     CDS             complement(43581..45761)
                     /gene="etk"
                     /locus_tag="RH222_000919"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV3795977.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGINVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(45781..46227)
                     /gene="etp"
                     /locus_tag="RH222_000920"
                     /gene_synonym="yccY"
     CDS             complement(45781..46227)
                     /gene="etp"
                     /locus_tag="RH222_000920"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV3795978.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46215..47354)
                     /locus_tag="RH222_000921"
     CDS             complement(46215..47354)
                     /locus_tag="RH222_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV3795979.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(47400..49496)
                     /locus_tag="RH222_000922"
     CDS             complement(47400..49496)
                     /locus_tag="RH222_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV3795980.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGHWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(49496..50242)
                     /locus_tag="RH222_000923"
     CDS             complement(49496..50242)
                     /locus_tag="RH222_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV3795981.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50239..50883)
                     /gene="gfcB"
                     /locus_tag="RH222_000924"
     CDS             complement(50239..50883)
                     /gene="gfcB"
                     /locus_tag="RH222_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDV3795982.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50990..>51283)
                     /locus_tag="RH222_000925"
     CDS             complement(50990..>51283)
                     /locus_tag="RH222_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3795983.1"
                     /translation="LSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTGATTT
                     AATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        12675 a        12665 c        13522 g        12421 t
ORIGIN      
        1 atatttttta agagtgacta tttatgaaaa cttgcataac aaagggaatt gtgaccgtaa
       61 gtttaacggc aatattactc agctgctcat ccgcatgggc ggcaggtaaa ggcgggattg
      121 gacttgcagc aacacgtctt gtttattcag aaggtgaaga gcaaatttca ctgggtgtac
      181 gtaacaccag tccggatgtt ccttatctta ttcagtcatg ggtgatgacc ccagataata
      241 aaaaatcagc agattttatt attacaccac cgctatttgt gctgaatccg gctaatgaga
      301 atctgttacg cattatgtac attggtgcgc cgttggcgaa agacagagaa acccttttct
      361 tcactagcgt acgggcagtc ccttcaacaa cgaagcggga agagggaaat accctgaaga
      421 ttgccacaca aagcgtcatc aaacttttct ggcgaccaaa aggtttagcg tatcccttag
      481 gcgaggctcc ggcgaaactg cgttgcactt cgtcagctga catggttacg gtcagtaacc
      541 caacacctta tttcattacc ctgacagacc tgaaaatagg tggaaaagta gttaaaaatc
      601 aaatgatttc cccctttgat aaataccaat tttctctgcc aaagggggcc aaaaatagca
      661 gcgtaacgta tcgaaccatc aatgactacg gggcggaaac gccgcaactc aactgtaaat
      721 cgtaagccgt cttcagttaa gagagcgaga tgtatagaac tcatcgacaa cacagcctgt
      781 taagctctgg tggagtgcca tcgtttattg gtgggctggt ggtgtttgtg tcggcagcgt
      841 tcaatgcaca agctgaaacc tggttcgatc ctgctttttt caaagatgat ccctcaatgg
      901 tggccgattt gtctcgtttc gaaaaaggac aaaaaataac gccaggggtt tatcgtgtcg
      961 atattgttct gaatcagaca attgtagata cgcgcaacgt caattttgtt gagataacgc
     1021 cagagaaggg gattgccgcc tgtttgacga ctgaaagcct ggatgcaatg ggtgttaata
     1081 ctgatgcgtt tccggctttt aaacaactgg acaaacaagc gtgtgtgcca ttggcggaga
     1141 ttattccgga tgccagcgta acgtttaatg tgaataaact ccgtctggaa atttcagtac
     1201 cgcaaatcgc catcaaaagt aatgcccgtg gttatgtccc ccctgaacgt tgggatgaag
     1261 ggatcaacgc gctattactg ggatattcgt ttagcggggc taacagtatt catagcagcg
     1321 cagacagtga ttctggcgac agctattttc tgaatttaaa cagtggcgtt aatttaggcc
     1381 catggagatt gcgcaacaat tcaacatgga gtcgcagtag tggccaaacc gcagaatgga
     1441 agaatctcag cagctatttg cagcgggcgg ttattccact aaaaggcgaa ctgaccgtat
     1501 gtgatgatta tactgcaggc gattttttcg atagtgtcag ctttcgtggt gtgcagctgg
     1561 cgtcagatga cgacatgctg ccagacagcc tgaaagggtt tgcgcctgtg gtgcgtggta
     1621 tcgccaaaag caatgcccag ataacgatta agcaaaatgg ttacaccatt taccaaactt
     1681 atgtatcgcc tggtgctttt gaaattagtg atctctattc cacgtcgtcg agcggtgatt
     1741 tgttagttga aatcaaagaa gcggacggca gcgtcaatag ctacagcgta ccgttttcca
     1801 gcgtgccatt actccagcgt caggggcgaa tcaaatacgc ggtgacactg gcgaaataca
     1861 gaaccaatag taatgaacag caggagagca aatttgccca ggccacgttg cagtggggcg
     1921 gaccgtgggg aacgacatgg tatggtggtg gacaatatgc tgaatattac cgtgccgcca
     1981 tgtttggtct gggatttaac cttggcgatt tcggagcaat ttcgttcgat gcgacccagg
     2041 cgaagagtac gctggcagac caaagcgaac ataaaggtca gtcatatcgt tttctgtatg
     2101 ccaaaacgct caaccaattg ggcactaatt ttcaattgat gggctatcgc tattcgacgt
     2161 cgggtttcta caccctttcc gacaccatgt ataaacatat ggatggctac gaatttaatg
     2221 acggtgatga tgaagatacg ccgatgtggt cgcgttatta caatttgttt tacaccaaac
     2281 gtggcaaact gcaggtcaat atctcccagc aattaggcga gtacggttcg ttttatttaa
     2341 gtggtagcca gcaaacttac tggcataccg atcaacagga tcggctatta cagtttggct
     2401 acaacacgca aattaaagat ctctcgctgg gggtttcctg gaactacagt aagtcccgtg
     2461 gtcaacctga tgctgatcag gtgtttgcac taaatttttc cctgccgctc aatctgttgc
     2521 tccccagaag taatgatagc tataccagga aaaaaaatta cgcctggatg acctctaaca
     2581 ccagtatcga taacgaaggg cacattacac aaaacctggg tttaacggag acactactcg
     2641 atgacggtaa cctgagctac agcgtgcaac agggatataa cagcgagggg aaaacggcta
     2701 atggtagcgc cagcatggac tacaaagggg cgtttgcaga tgcccgagtg ggctacaact
     2761 acagcgataa cggcagtcaa caacaactga actacgctct ttcaggcagt ttagttgccc
     2821 attcacaggg cattaccctg gggcaatcgc tgggggaaac taacgtgctg attgcagcac
     2881 caggcgcaga gaatactcgt gtggcgaaca gcaccgggct gaaaactgac tggcgcggat
     2941 ataccgttgt cccctatgcc acttcttatc gggaaaatcg aatcgcactt gatgcggcat
     3001 cgttaaaacg taacgtggat cttgaaaatg cagtagtcaa cgtggttccc accaaagggg
     3061 cgttggttct ggcggagttc aatgcccatg cgggagcaag ggtattaatg aaaacatcaa
     3121 agcagggtat accgctgcgt tttggcgcga tagcgacgct ggacggcata cagactaata
     3181 gcggcattat tgatgatgat ggctcgctct atatgtccgg tttgcccgct cagggagcga
     3241 taactgtacg ctggggcgaa gctcccgatc aaatttgtca tatcagttac cagcttaccg
     3301 aacaacaaat taactctgcg attacgcgaa tggatgccat atgcagataa tctttggaga
     3361 aaaatgcgtg ttattactac gactattttt tgccgccgtc ttaatgctat ggtgcgctca
     3421 aactgctgct tatagcgggc agtgtcatac cactcagggg aatccgtata ttggcgtcaa
     3481 ttttggcgtt aaaaccctgg aggaagaaga aaatacggct ggagtagtta aagacaaatt
     3541 ttatcagtgg aacgaatcga atgattatta tgtttcctgt gattgcgata aagacaatgt
     3601 cagaagtggc cgatgggcat tcgccgcgga ttcaccgtta gtctatttag gcgacaactg
     3661 gtacaaaatt aatgactatc ttgccgccaa agttttattg caggttaaag gcagttctcc
     3721 tactgcggtt cctttcgaaa acgtgggcac aggggcagat acacgatggc atatttgcga
     3781 tcccggcggt caacgtttag gtggccaggg ggctagcggt aatagcggta gcttttccct
     3841 gaaaatattg cagccgttcg ttggttcggt cgtcattcct cctatggcgc tggcgcgatt
     3901 atttgaatgc tacaacatac ccgcaggtga ttcctgcacg actacaggta caccggtttt
     3961 agtgtattac ctgtctggta cgatcaattc acttggctca tgttccgtca atgccggaga
     4021 aacaatcgag gtcgatctgg gcgacgtatt tgcggctaac tttcgtgttg tagggcataa
     4081 gcctcttggg gccagaacgg cagaacttgc aattccagtc aggtgtaaca cgggaaacgc
     4141 ggggttagtt aacgtcaacc tgagtctgac ggcaaccaca gaccccagct atccccaggc
     4201 gattaagacg tcacgtcctg gcgtgggcgt ggtggtgacc gatagccaga acaacattat
     4261 ttcccctgct ggtggaacat taccgctctc tattcctgat gatgcagaca gtatcgcgcg
     4321 aatgaatgtc tatccagtca gcacgacagg tgtaccacca gaaaccgggc gatttgaagc
     4381 cacggcaacg gtgagaataa attttgatta acgcgtgaac gtatgaagaa aaaaacgata
     4441 tttcagtgcg ttatcttgtt ctttagcatt cttaacatcc atgtcgggat ggctgggcct
     4501 gaacaagtta gtatgcatat ttatgggaat gtggtcgatc agggctgtga tgtcgccacc
     4561 aaaagtgcat tacaaaatat tcatattggt gattttaata tcagtgattt tcaggccgcg
     4621 aataccgtaa gcactgctgc tgatttgaat atcgatatca ccggttgtgc cgctggtatt
     4681 actggcgcgg acgtcctttt tagcggcgag gctgacaccc ttgcgccgac actgctcaaa
     4741 ctaactgaca caggcggaag cggtggtatg gcaacgggga ttgccgtgca aattcttgat
     4801 gcgcaaagtc agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc
     4861 ggggataaca cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc
     4921 ggtaatgcga cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa
     4981 acggataatc tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca
     5041 tgatggcacg gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga
     5101 tagcaagcaa atcaatgtgc agttaggcga tgtttcgctg acacgttttt ctcatgggaa
     5161 ttatggcccg gagaaatctt tcatcattaa tttgcaggat tgcggaactg atgtcagcac
     5221 ggttgacgtc accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga
     5281 aagtggtacg gatgcagcca gcgggctggc aattgcgatt ctggatgatg cgaagatatt
     5341 gattccgctt aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac
     5401 tttttatgcg caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag
     5461 cgcgacattt gtacttcatt atgactaata cgtggaacag attggcgctc ttgattttcg
     5521 ccgttttatc gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc
     5581 gatttatctt tccggcagac agagaatcga tatctatttt actgactaat accagtcagg
     5641 aatcctggct tattaacagc aaaatcaacc gcccaacgcg ttgggccggg ggggaagcgt
     5701 cgacagtgcc agcaccatta ctggccgctc cgccacttat tctcctgaag cccggtacta
     5761 caggcacgtt gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc
     5821 tatttgagtt aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag
     5881 tggcgatacg ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc
     5941 tggaagctta tcaacaatta cgctggacat ggaattcgca gggtgtacaa ctcactaacc
     6001 caacgcctta ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg
     6061 cgggagttgt gccgcctaaa agccagcgtc agacaagctg gtgtcaggca atcgcaccgt
     6121 gtcatgtcgc ctggcgggcg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa
     6181 atctgccctg aaacaggttc ggaaaacgtt tgcgtttttt ttgccgcagg tcaattccct
     6241 tttggtccga actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt
     6301 catgtactac cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga
     6361 gtttactttt cagcaattac gccgtattac aggaacgccg tttgaagcac tggtgcggca
     6421 gaaagtgcct gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct
     6481 ggcagccggt cttgataaag acggggagtg cattgacgcg ttaggcgcga tgggatttgg
     6541 atcgatcgag atcggtaccg tcacgccacg tccacagcca ggtaatgaca agccgcgtct
     6601 ctttcgtctg gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt
     6661 tgataacctc gtagagaacg taaaaaaggc ccattatgac ggcgtcctgg gtattaacat
     6721 cggcaaaaat aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga
     6781 aaaaatctat gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg
     6841 attacgcaca ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa
     6901 gcaaaatgat ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc
     6961 ggatctttct gaagaagaat tgatccaggt tgccgatagt ttagttcgcc ataatattga
     7021 tggcgttatt gcaaccaata ccacactcga tcgttctctt gttcagggaa tgaaaaattg
     7081 cgatcaaacc ggtggcttaa gtggtcgtcc gcttcagtta aaaagcaccg aaattattcg
     7141 ccgcttgtca ctggaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc
     7201 ggttatcgct gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg
     7261 ttttattttt aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct
     7321 tcgaattcct ctttataaga taatcagggc tttattttca gccctggttg ttttatattc
     7381 attgctgttg cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga
     7441 caatggattg tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca
     7501 gacgataact ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc
     7561 aatggtatgc tatttcgctc acgttttgcg cgcaagatgt tgactccaga tgccttttct
     7621 cccgcaggct tttgcgttga cgacgccgcg ctctatttct cttttgaaga aaagtgccgc
     7681 gattttaatt tatctaaaga acaaaaagcc gaactggtgc tgaatgcact ggtagcaatt
     7741 cgctatctca aaccgcaaat gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg
     7801 gtgcccatgc cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat
     7861 ttgctggtcg ttgaatctgg cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt
     7921 gttattgcgg gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg
     7981 aaaccgcagg ttaatgttga cagcttcagc ctcgaacagg cagtctaacg cgccaactta
     8041 agtgcagtct tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat
     8101 tttttcagcg gcgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc
     8161 gcgcggcaag aataagggat acgaattccc tgattttcta attgctccag caacacctgt
     8221 tggttatttc cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt
     8281 tgcgtgatgt tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt
     8341 gccagaattt ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc
     8401 tgaccaaaat cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat
     8461 ggttcgcctg ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg
     8521 ctacaaggtt taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagcgatct
     8581 tcttcccagg ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca
     8641 ctggccggac aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga
     8701 tagccatcag caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt
     8761 ggccccaccc agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc
     8821 gcgtctggcg caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt
     8881 gtggcgaaat cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag
     8941 ccatcatgca caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata
     9001 aacgtaccgt caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca
     9061 gccagagcat gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg
     9121 gttaatgtca ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac
     9181 gcgcttatta gctataatgc gcaacaattt tcttagcgcc tgtcccatta ggctatttta
     9241 tttgtcagtt tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt
     9301 tctccgcgct gtccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt
     9361 agagtaaaag tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc
     9421 tgttaaaaac tgaactggaa aacctggggg ccgttgaatg ccaggtggtt cagggtgggg
     9481 tccatttcaa gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct
     9541 cgcgtattat gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg
     9601 gtgttcaggc gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact
     9661 tcagtggttt gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg
     9721 cgatcgtcga tgctttcacg cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc
     9781 cggatatccg cgttaacgtc tggctgcata aagaaaccgc cagtatcgcc cttgatctca
     9841 gtggtgatgg tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag
     9901 aaaccctggc agccgctatt gtgatgcgat ccggctggca gccaggaaca ccgctgctcg
     9961 atccgatgtg tggttccggt acgttgctga ttgaagcagc gatgctggcg accgatcgcg
    10021 cacctggctt gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta
    10081 tctggcagga agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggcctg gctgagtata
    10141 gctctcattt ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg
    10201 cccgtcttgc ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga
    10261 ccaatccgct gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac
    10321 gtctggacag cgaaccggcg ctgatagcgc tgcatagtct gctgggccga atcatgaaaa
    10381 accagttcgg tggctggaat ctctctttgt ttagtgcctc gccggatctg ctcagctgcc
    10441 tgcagctgcg tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga
    10501 aaaattacca cgttgccgaa agcactccag acagcaaacc ggcgatggta gcggaagact
    10561 acgctaaccg cctgcgtaag aacctcaaaa aattcgagaa gtgggctcgc caggaaggga
    10621 ttgaatgtta ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt
    10681 atgccgactg ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag
    10741 cgcgtcagcg tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa
    10801 acaaactggt gctgaaaacc cgtgaacgcc agaagggcaa aaatcagtac cagaaactgg
    10861 gcgagaaggg cgagtttctc gaagtcaccg aatataacgc tcacttgtgg gtgaacctga
    10921 cggattacct cgataccggt ctgttcctcg atcaccgtat cgcccgtcgt atgctcggtc
    10981 agatgagcaa aggcaaagat ttcctcaacc tgttctctta taccggcagc gccaccgtgc
    11041 acgcgggatt aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg
    11101 agtgggcaga acgcaacctg cgtctgaatg gcctaaccgg gcgtgcgcat cgcctgattc
    11161 aggccgattg cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg
    11221 atccgccaac cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc
    11281 atctggcgct gatgaaagat ttgaaacgtc tgctgcgtgc tggtgggacg atcatgttct
    11341 cgaacaacaa acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag
    11401 cacaagaaat tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca
    11461 actgctggct gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg
    11521 cgcatggctg tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga
    11581 taacgaacgt gtttgtctgg tgggccgcaa cggcgcaggc aaatcgacgt taatgaaaat
    11641 cctcaaccgt gaacaagggc tggatgacgg tcgcattatt tacgagcagg atttgattgt
    11701 agcgcgtctg caacaggatc cgccgcgtaa cgttgagggt agtgtttatg atttcgttgc
    11761 cgaaggcatt gaagaacaag cggaatatct gaaacgctat cacgatattt ctcgcctggt
    11821 gatgaacgac ccgagcgaga aaaatctcaa cgaactggca aaggttcagg aacagctgga
    11881 tcaccacaac ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt
    11941 agatcctaac gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg
    12001 acgcgcgctg gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accacctgga
    12061 tatcgaaacc atcgactggc tggaagggtt cctcaaaacc ttcaatggga cgattatttt
    12121 catctcccac gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg
    12181 cggcaagctg gtgacctatc cgggcaatta cgatcagtac ctgctggaaa aagaagaagc
    12241 cctgcgcgtg gaagaactac aaaatgccga gttcgatcgc aaactggcgc aggaagaagt
    12301 gtggattcgc caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct
    12361 gaaagcgatg cgtcgcgagc ggggcgaacg tcgcgaagtg atgggtaccg caaagatgca
    12421 ggtggaagag gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctatca
    12481 ggttgacggt aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat
    12541 tgccctgatt ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactaa tgctcggtca
    12601 gcttcaagca gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga
    12661 tcagcaccgc gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa
    12721 gcaagaggtg atggttaacg gcaagccacg ccacgtattg ggctatttgc aggacttcct
    12781 gttccatcca aaacgggcga tgacgccggt acgtgcgctt tctggcggtg agcgtaaccg
    12841 cttgttgttg gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac
    12901 caacgatctt gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg
    12961 cacggtattg ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg
    13021 gatcttcgaa ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg
    13081 tcagcaagaa cagtatgtgg cgctcaaaca gcctgcggtg aaaaaaaccg aagaagccgc
    13141 cgtgccaaaa gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg
    13201 cgaactggag cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca
    13261 gacgcaggtg gcggatgctt ccttcttcag tcagccgcat gagcagacac aaaaagtgct
    13321 cgccgatatg gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct
    13381 tgaagcgtta aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac
    13441 aacggtaaaa ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt
    13501 catctgtaaa atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa
    13561 gcacatcctg tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca
    13621 gaaagcggca tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca
    13681 gcgtccgacc gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc
    13741 ttttgtgaat atgaacgttg caggagttac cagtgaaata acattactgg aaattcccgg
    13801 cgtgcttttt tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact
    13861 ggttcccgcg ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt
    13921 ccgtttaaaa gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga
    13981 gattttcctc gctggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg
    14041 ggtaggcagc agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca
    14101 gtgcgttgat cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc
    14161 gctaaaacca ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc
    14221 aatccttcct gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg
    14281 tagaaacagc ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc
    14341 ggctaatatt ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat
    14401 tctcgctggg gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt
    14461 tctggccagt attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga
    14521 tgccaaaggg catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga
    14581 gtttgtaggc cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt
    14641 gcgtatggga ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt
    14701 cgtcataatg acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca
    14761 acctgaatca gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa
    14821 tccagaaagt gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg
    14881 gggcctgggt tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg
    14941 cgaatgcgga aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg
    15001 gcgtggttga aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc
    15061 tgaattccgg tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc
    15121 agattggtcg cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac
    15181 tgcagccagg cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcgccaccgt
    15241 tggccccgcc tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc
    15301 tctcgccagg agatccggtg cttttccgtg gctatcgggt aggttcggtt gaaaccagca
    15361 ccttcgatac gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc
    15421 gactggtgac cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt
    15481 cagcggggat gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct
    15541 ttgatgtgcc ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt
    15601 tgtatgatga tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga
    15661 tgttctttaa agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta
    15721 ttcgcctggg taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta
    15781 acgatgatta ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc
    15841 ttggcgaaaa tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg
    15901 gatcgctgaa aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc
    15961 caaatacgcc tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg
    16021 ttagcggcgg cctggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca
    16081 aactgccatt gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca
    16141 caatgaaaaa cctgcaaacg acgctggata gcatgaacaa gatcctcgct agccagtcga
    16201 tgcagcagtt gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg
    16261 gcttccagcc tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc
    16321 aggtgttgcg agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg
    16381 tatttgaagc gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg
    16441 gctagtgacg attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa
    16501 ctattaccag ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg
    16561 tctgttatgg gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta
    16621 tcaaaccagt gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga
    16681 tcaacagttg cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt
    16741 tgcctcccag cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa
    16801 cggtcgctat gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca
    16861 actgatcaaa cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat
    16921 ggttaaagtg ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa
    16981 gcgtctacct taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc
    17041 ggtttttttg tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg
    17101 acatgcccgt gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt
    17161 atgattcgcg gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca
    17221 ccagaaacca gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg
    17281 ggcacatcaa cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta
    17341 tcagacgctg aatcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag
    17401 ggtagtaatg gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc
    17461 cttttgatac tctgtctgat tataatcaga aggcagacgt atcctggaac agaccgactt
    17521 tcaggtcgct ggcggtatag ataagacgac catcaaccag cacttcgcca tccgccaggc
    17581 ccataatcag acgacggtta acaatgcgtt taaagtgaat acggtaggtc acttttttcg
    17641 ctgtcggcag tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac
    17701 cttcgccgcc cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca
    17761 agcatcccgg cataaccgga tcgccaataa agtggcatcc gaagaaccac agatccggat
    17821 tgatatccag ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt
    17881 tgaccacacg gtccatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa
    17941 acagttcacc gcgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta
    18001 ccatgttctc tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct
    18061 gaacaagtcc gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg
    18121 gtgacgtcct tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt
    18181 cgtttggcct tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc
    18241 gtctactgcc cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag
    18301 acttaaatgg cgaacgttag ctgtggggat aatgacacct tgtttcccgg ttaactcacg
    18361 ttgctggcaa atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctgggc
    18421 gcgaccgaac tgatcgactg aacctgtgat agcgatactc tgattcaccg gcacatcggc
    18481 gagggcgctt atcagggcgc agagttcagc catcgaggca ctatctccat caacttcact
    18541 gtatgactgc tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc
    18601 cgacatcagg aacgcttgca tgatcatcat ccctttcgca tggatattgc cgccaagctc
    18661 cgctttgcgt tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg
    18721 agaaggttcg ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat
    18781 ttgcccgatg cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg
    18841 catacgttca gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg
    18901 ctcgccggag aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat
    18961 ccactgcggg ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat
    19021 aagtaccggc caggcatccg ctcccggtgc aggtaagtga ttatgtctgg cggtaaatgt
    19081 cacccagcga caccactggg ttactgactc tgcatcgaca atctgcagag tatcttcaaa
    19141 ttcgctataa atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga
    19201 ttcgcgttcg cctaccagaa tgactttcag cttcaatggc atcgaaggca cagagacggg
    19261 gagagggcgc gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt
    19321 cagccgcatc cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc
    19381 gccatttgcc tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag
    19441 gcagccaaag agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc
    19501 aaaattatca tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata
    19561 gtgaccgccc accagttggc ctgcatcgct ttgtagcgta cgcgcggcat tggcaataag
    19621 attcagatac tcagactctt ccggggcctt cgccagcata aaagaggagg atgctcgcgt
    19681 atgcagcaac tgctccagcg caaattgcag ccgcggttga gtatcactga ataaaggatc
    19741 gttttcgtca atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac
    19801 caggtcacgc catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa
    19861 agccgcgatt ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct
    19921 ggcatgggcg ttaaagctca caggatggag attcttttct tcactggcct aaaaagctga
    19981 tattctgtaa agagttacac ggtaacattg agatcgctat gaaatatcaa caacttgaaa
    20041 atcttgaaag cggttggaaa tggaagtatc tggtgaagaa gcatcgcgaa ggggagttaa
    20101 tcacccgtta catagaagcc agtgccgccc aggaagccgt cgatgtgttg ctctcgctgg
    20161 aaaatgaacc cgtgctggta aatggctgga ttgacaagca tatgaatccg gaactggtca
    20221 atcgcatgaa gcaaaccatt cgggccagac ggaagcgcca ttttaatgcg gaacaccagc
    20281 atacgcgcaa aaaatcgatc gatctggaat ttattgtctg gcaacgtctg gctggtcttg
    20341 cgcagcgtcg cggtaaaacg ctttctgaaa cgattgttca gttgattgaa gatgcggaaa
    20401 acaaagagaa atacgcgaat aaaatgtctt ctctgaagca ggatctgcag gcattgctgg
    20461 gtaaggaata actgacgaaa gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca
    20521 gcggggtttt tctaccagac gagaacttaa gcttgcggct gagttacaac gtctttgata
    20581 cctttaactt cgatctctac gcgacgatcc ggagccaggc agtcgatcag tgcagcacgc
    20641 tgtttcacgt tgtcacaggt gttgccagta accgggttgg attcgcccat accacgtgcg
    20701 gagatcttgt ctgccgggat acctttggag atcaggtaat caacaacaga ctgagcacgg
    20761 cgctcggaca gaccctggtt gtaagcgtca gaaccgatgc ggtcggtgta acccagaaca
    20821 actacggaac cgtctttcgg atccaggttg ctcagctggc tgtacagctg atccagagca
    20881 gcctgacctt ccggtttcag ggttgctttg ttgaagttga acagaacgtc agacttcaga
    20941 gtgaagtgct tggtctgtac ttccggtgcc ggagctggag ccggagcaac tactggagct
    21001 gcttcgccct gaccgaaacg gtaggaaaca cccaggctca gcatgccgtt gtccggacga
    21061 gtgccgatgg tgtgtgcgtc accgatgttg ttggtccact ggtattccag acgggtagcg
    21121 atttcaggag tgatcgcgta ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg
    21181 tggtctttaa aggatgcgcc accaggtacg ttagccttgg tgtctgcacg ccataccata
    21241 ccacccagac gagtgtatac gtccagatcg tcagtgattg ggtaacccag tttagcagtc
    21301 agttgaacgc cctgagcttt gtatgcaccg ttgatgttgt cgcctttgta cggcatacga
    21361 cctaaccagt cgtaacccat ttcaaaacca acatacgggt taacctggta accaccaaaa
    21421 gcacctgcac ccagttggtt ttcgtgggtc ggaccattgt taggaataaa accagtgtca
    21481 tggtactggg accagcccag tttagcacca gtgtaccagg tgttatcttt cggagcggcc
    21541 tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg cgatagctgt ctttttcatt
    21601 ttttgcgcct cgttatcatc caaaatacgc catgaatatc tccaacgaga taacacggtt
    21661 aaatccttca ccgggggatc tgctcaatat taactctacc gatatcttcg gcttatgccg
    21721 agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa cttacaagtg tgaactccgt
    21781 caggcatatg aaaaaaaagt cttgtataag gtatgtttaa tcttttttgt cagcgacaat
    21841 ttacagaaga aaatcgcgga aaccgcttca gacaagcctc cgcaaggaaa attagtcacg
    21901 actgaaagca ttggctgggc gacaaaaaaa gttccaggat taatcctaaa tttacttaat
    21961 gatacaaatt agagtgaatt tttagcccgg aaagttgtct cgtggcgtga gaggatgcgc
    22021 ttaccggacg cataataaac cccatagcgt taccttcatt tgccgcatca acaagttcag
    22081 catgctcttc ttcagtcaaa tcatctgcca accaaccgat caccacactg taattgcccg
    22141 tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg ggagagctgg ctaatctgca
    22201 ttactttcgt taagggtagc ccagatgcct gaacccattc ccgactcagt ttttgttgcg
    22261 gtgttaacca gagttgccag cgcgattgct gaccgagttg ctgtaacaat ggcaacagta
    22321 gaagttgcgt catcatgggc tgatcttcgc gatagacaac ttcactgata agcccggctg
    22381 tagtgttttc cgtagagaca cgcgcaattt tacttgctgc ggatgagaac gacgaagaac
    22441 gatgtgcata gcctgaagtg tacataatca atccagcccc tgtgagttac tgtatggata
    22501 tacagtacat ccagtgacaa caaagatcaa ccctattttc ggaaagagcc tcgcaaattt
    22561 tgtcgttggt gacgggaaaa cataaattaa tcttgcccct taagaataag ttgcctattt
    22621 tcgtagttaa cggatccgtt aatgtgaatc attcttttat gttatgattt taaaaggaat
    22681 tttatgaaaa gcctctccta taagcggatc tataaatcac aagaatacct ggcaacgttg
    22741 ggcacaattg aataccgatc attgtttggc agttacagcc tgaccgttga cgacacggtg
    22801 tttgcgatgg tttctgatgg tgagttgtat cttcgggctt gtgagcaaag tgcacagtac
    22861 tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt gtggccgatc cgttaccctc
    22921 aattactatc gggttgatga aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg
    22981 aaatattctc tcgatgcagc gctgaaagag aaaagcacgc gcaatacccg ggaaagactg
    23041 aaagatttgc ccaatatgtc ttttcatctg gaagcgattc tcggggaggt ggggattaag
    23101 gatgtacggg cgttacgtat acttggggca aaaatgtgtt ggttgcgact gcggcagcaa
    23161 aacagtctgg tgacagaaaa gattctgttt atgcttgaag gtgccattat cggcattcat
    23221 gaagctgcgc tcccggtggc acgccgccag gagcttgcag aatgggctga ctctcttacg
    23281 ccgaaacagg agtttcctgc ggaacttgag taatctggcg ttgcagacga ccaatctcag
    23341 gcagtaatgc aatcaataat ccaacttgtt gtacgaccag aggttctttg ctgtctgccc
    23401 gtggttcaag ttgctgcatc cgctgtttca ggctagctaa tgcctcattg acgcgttctt
    23461 catcagcagg ttgatgatgt aacgcgtcat caacatagca aactgcgtca tcaagaaacg
    23521 ccagaatttc aggattagtt aactgctccc ggtgagcacc gagggctgag atatagctgg
    23581 taaacgtatg gttaaggcac agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt
    23641 tcggctcgct ggacatattt gataccaccg acgccagctc agcatcacgg ttgtgtgcat
    23701 cgcggcgggc aatacgatac gccagacggt tatcacgccc ctgatggtat tgctccagta
    23761 tggcatcgag ataccgacag ttggcctctg tggcgcgttc gagcatgcgc ggcagattgc
    23821 gaaactgcca gtcaggccag atgtagctca ctgccgccca cgcaatggca caaccaatca
    23881 gcgtatcgat tacgcgaggt aacgctactt caaaaccttc acccagtaag ttaaaacaca
    23941 gtagcaccaa aagtgtgatg aacatcgttg catgagcgta ttgcacgtta cggaaggcaa
    24001 aaaagagcac gccggtaata accagcagca ccagctgccc ttccagtgat ggcacaaacc
    24061 acagcacagg aatgccaatg gcgataccta ccagcgtacc aataatcctt aacttcaggc
    24121 ggtggcgcgt ggcgttatag tttggctggc agacaaacaa acttgtcagc aagatccaat
    24181 acccgtgatg tattccggtt atttgaatga tggcgtagcc gaagcacaac accagcgaca
    24241 ttcttaccgc atgacggaag agggcggatt ccggcgtgaa gtgacggcta agacgcagcc
    24301 agatatcact caacccgtgc gggctgtcat cagcgagctc attttcgtca ttattatggg
    24361 gtagtgcctg ggcctgttct gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat
    24421 tgttcagcaa aaatcccagt gttttgagta aatcggcggg tgcgccgtta tcgcgcatcc
    24481 gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag
    24541 gctgacgcag caaaatacag cgtgacagtt gctggcacgc ctggccctgc atcgacatca
    24601 gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact
    24661 gaatatgaga agagctggca cgctcgtgaa tatcctgcgc gacaaagtaa taatgcagcg
    24721 tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg
    24781 tctgattcaa tgtcgccatc agttgtccgt tggcgagagc caaatcgtac agcggtgcct
    24841 ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca
    24901 gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg gaccgggaac agcagatgac
    24961 caataagtgt caggacgttg taccagacgg caccggccag cagatacatc ggctgctgat
    25021 accagtgctc atacagtgat gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga
    25081 aggcaattgt tgcatagcgt tgacccagac cgccgagcaa aatgaagccg ctggtagaga
    25141 gcgttaagcc aatcgcaaat agccagggcc agggaaacag caattctact gaggccgagg
    25201 cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat
    25261 cgagatcggt cagcgccgct gccaccatcc ccagcgttag cggaatcgtc agttttacat
    25321 cacccagcca ccacggaaac gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat
    25381 acagccaggc gctgttccag gtatagcgtt tgagcaaagg acttagcata aaggccatat
    25441 tagtggatta ttcaaaacga cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta
    25501 ccgataccac gcgacgacca accggccata gtgcaatagc ggcaatttta aagttcgcaa
    25561 tgccaacagg aatgccaatg attgaaatac attgtgcgat gcccgttgca atgtgcatca
    25621 ggcataacca ccagccaaag aaaatcagcc agaaaatatt caataccgta ccgccagtat
    25681 tcagcagcac atttttgcca gccgggttca gttcatcgac atgaatagct tcattgccat
    25741 aaggcaccag agacagttta gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa
    25801 tcagcacaat actgaccaga gtcgccaaca gccagcccag agtggtggca aatccgccaa
    25861 gcacaaagtt cagaatgttc aaaacggtac gcataaaacc tcgctttact gtggttttca
    25921 gtaatgggcg gcaattgtaa cgtttttttg ggctggagca cgttttctct gacggttaca
    25981 ctgataagaa ataatttcgt gtggatctac agagtcatgg aactgaaagc gacaacgctt
    26041 ggaaaacgtc tggcacagca cccttacgat cgggcggtga tcctcaatgc cgggattaaa
    26101 gtctccggcg atcgccacga ataccttatt cctttcaatc aattactggc gattcactgt
    26161 aagcgcggtc tggtatgggg cgagctggaa tttgtactgc cggacgaaaa agtggtgcgt
    26221 ctgcacggca ccgaatgggg cgagacgcag cgtttttacc atcatcttga tgctcactgg
    26281 cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg ttttacgcca gcaactggat
    26341 ttgattgcca cgcgcactgg agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt
    26401 cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc cggttaaccg actggaagaa
    26461 ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct ggttgaaaga tattgaaagc
    26521 gctcggttgc agcataacca ggcgtatacc gaagccatgc ttaccgagta tgcggatttt
    26581 ttccgccagg tcgagtcttc accgctgaat ccggcgcagg cccgggcagt cgttaatggc
    26641 gagcattctc tgttagtgct ggcaggtgca ggaagcggaa aaacgtcggt gctggtggcc
    26701 cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg
    26761 tttggtcgca aagccgctga agagatggac gagcggattc gcgaacggct acataccgaa
    26821 gacattaccg cacgcacgtt tcatgcgctg gcgctgcata ttattcagca gggcagcaaa
    26881 aaagttccga tagtcagcaa actggaaaat gataccgctg cccgtcatga actctttatt
    26941 gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg
    27001 ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact tctgggatga tgaaaaatta
    27061 cagcgtcgcc ttgcctctcg cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt
    27121 gcacaggcag aaatgattgc cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt
    27181 atcaagttga tggccccgtt attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct
    27241 gtcgattttt cgggccttat tcatcaggcg attgtgattc tggagaaagg tcgctttatc
    27301 agcccgtgga agcatattct ggttgatgaa tttcaggata tctcgccgca gcgggcagcg
    27361 ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt tgttcgctgt tggtgatgac
    27421 tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa
    27481 aactttggtg aaggcgaacg ttgtgattta gacacgactt accgttttaa cagtcgtatc
    27541 ggtaaggtgg caaaccggtt tattcagcag aacccaggcc agctgaaaaa gccgctaaac
    27601 agcttaacca atggagacaa aaaagccgtc acgttattgg atgagagtca acttgacgct
    27661 ttgctggata agctctctgg ttatgccaaa ccggaagagc gcattctgat cctggcgcgt
    27721 taccatcaca tgaggcctgc ccgcctggaa aaagcggcaa cacgctggcc gaagttgcaa
    27781 atcgacttta tgaccattca tgccagcaaa gggcaacagg cggattacgt catcatcgtt
    27841 ggcttgcagg agggaagtga tggttttccg gctgcggcgc gggaatcgat tatggaagag
    27901 gcgctactgc caccggttga ggatttcccg gacgcggaag aacggcggtt aatgtacgtg
    27961 gcgctgaccc gggcacgcca tcgggtatgg gcactgttta acaaagagaa tccctctccc
    28021 tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga gaaaaccgta agaaacaggt
    28081 ggcgtttgcc acctgtgcaa tattacttca gacggtccgc gagataacgc tgataatcgg
    28141 ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg gattatgaag tctgccgttg
    28201 ccacgttggt ggcgaccgga atgttccata ccgtcgccag acgcagcaag gctttcacgt
    28261 caggatcgtg cggcacggca tttagtggat cccagaagaa aatcaataca tcaattttcc
    28321 cttctgagat caatgcgcca acctgctggt caccccccat tgggccactc aacatcgcgt
    28381 tgacgttcat gccggtcgcg cgggaaatta agttaccggt agtgcctgtt gcatacagta
    28441 cgtgttgttc cagtaacggt tgatgccgtt ccacccagct catcagcatt tgtttgcagt
    28501 gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca
    28561 taatgtacat ccgtagttaa ctttcctaca gattactgta agcacttatc gctgcaagat
    28621 aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag gaagatggtt gtttttccgc
    28681 ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg
    28741 ttgttctgtg agcacctgag actgcggtgg aatagcatcc agctcggaaa cacccgaaag
    28801 cagcgtactg tcatctgcca tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga
    28861 tttgttatag cgttcaacct cgacctcata atcaatcgtt tttgccgttg aggtaatggc
    28921 gagaatatcc agctttaccg gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg
    28981 cgcggcatca aaatggttag cttcccgctc attctccagg cgaggcaggc gaaaattgac
    29041 ctggttgatg agctgggtat taaaactgac caccagcggt ggggagatat acagccgttc
    29101 ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac actaactgat gcggtccatt
    29161 atccagttca atgctgtccg caccgcgcag cagagagctg gaaaccttct tgccgtcgag
    29221 taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa
    29281 acataatgca atcaaggtgg tcacgatgcc ggttttcata ggtagctcct gtcaaaagac
    29341 cgcccgaata ataatgtgtc aaaatttttc tttaatcata cttactaaca tatagacata
    29401 tttcccgctt ttgctctcaa tcattcgtat tagctgcatg gttggcatgt cggctttgtc
    29461 gtacactttg ttaaacagtc tggaggaaat aatgaaagaa accgatattg caggtatttt
    29521 aacgtccact cacaccattg cgctggtggg ggcaagcgac aaacccgatc gtcccagcta
    29581 tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc attccggttt cgccaaaagt
    29641 tgccggcaaa acgctgctgg gccagcaggg gtatggcacg ctggcagacg ttccggaaaa
    29701 agtcgatatg gtcgatgttt tccgtaattc agaagcggcg tggggcgtgg cgcaggaagc
    29761 tatcgccatt ggcgcgaaaa ccctctggat gcaactgggg gtgattaatg agcaagcggc
    29821 agtactggca cgggatgccg ggttaaatgt ggtgatggat cgctgcccgg ctattgagat
    29881 ccctcgcctg ggcctggcca aataaaaaat ccccggaagg caaaaacctt ccggggattc
    29941 gttcagggat cagttacgca gacgcggggc ctggagttgt ttgcggatgg tctgcgccag
    30001 ctcatccatg gatggctgtt caggatgttc atcctgcaat tcgctactta gctgggcttc
    30061 agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc atcaccacgt gataccacgg
    30121 agcggcgcga agctcgtcat tcaccgccag ctcatcaggc gacggttcag agagcgaata
    30181 aaccgggtcg atatccacga ccactccgag ataacctaac agggaatggc ggacctgctg
    30241 gccgataccg aatttgctgg caatcatagt cacctcccgg gaaatcttta cctttgatat
    30301 aagggtaaaa ttccacattt caagttacat gacgcgacag gcaaaccctt tcagatatag
    30361 cccttccgga taggtagcga tcaccggatg atcggctgcc tgacggaact gctctataaa
    30421 ttgtacatca cggccggcat caattgcggc atccgcgatg attttctgaa ataaatcgct
    30481 ggtcatcaga ccggaacagg agaaagtcag gagaataccg ccttcattca gcaactgaat
    30541 cgccagcatg ttgatatctt tatagccacg acacgcgccc atcaactggc ttttattctc
    30601 aacaaacttc ggcgggtcca tcacgataac gtcaaatttt tcaccgcgat cgcgataagt
    30661 acgcagcaat ttaaagacat catcacggac aaactcagcc ttgctcagat ccagtttgtt
    30721 cagctcaacg ttctgccgtg caatatccag cgcttcctgg gaggtatcaa cgctgacaac
    30781 ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca ccggtatagg agaaacagtt
    30841 cagcacacgt ttattttcaa cgtagcggcg ggtagccagg cggctgtctc gctggtccag
    30901 gtagtagccc gttttgtgtc cgtgctgaat atccaccagc agtttcatgc cgtgttcttc
    30961 aatcggcagc agggcaggtg gcaactcgcc ggtgacgagg ccctgggtca gctccatccc
    31021 ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc gcacattccg ggtacagcgt
    31081 ttgcagggca ctaattaatg ccgcgcgctg atattctgcg ccagcactca gcagttgcag
    31141 caccagaaaa ttaccgaaac gatcgatagt aatacccggc aggccatcag attctccggc
    31201 gattaaacga tagctgtcga ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc
    31261 ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac tcagacgggt caaacgtcca
    31321 gacgcgcgcc cggatttgcg aagctggcga ataagcgccg cgtgctaacc attttccctg
    31381 atgatcaaca atatcgatgg tttcaccgag gctggcttta ccttccatgc gggcaacggc
    31441 cccggaaaag acccacggat ggcgacgaag taatgatttt tcgcgccctt tggctaacac
    31501 taaacgtaca ctcataattt acttttctgt cgatgcaaag aaatgggcgt cattgtccgg
    31561 agttttccat ggatttgcaa cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt
    31621 ctgcataatt gcctgggttt acgggcgagt tcagggcgta ggatttcgct acaccacaca
    31681 gtatgaagcg aaaagactgg gtttaaccgg gtacgccaaa aatcttgacg atggcagcgt
    31741 ggaagtggtt gcctgcggtg aagaagggca ggtggaaaaa ttaatgcagt ggttaaagag
    31801 tggcggcccg cgttctgcgc gtgtggaacg ggtgcttagc gagccgcatc atccctcggg
    31861 ggaattagcc gattttcgta tccgttaaat acattttacc ggttttggca ggccagcaat
    31921 tttggtggct tgcttagccg gacctttcgg aaacagtcga tataaatagc ggctgttacc
    31981 tttttcttcg ccaaatttat tcgccatcgc ttttaccagc atacgaatcg ccggagaagt
    32041 attgaattcc agatagaaat cacgcacaaa acgcaccact tcccagtgtt ctggcgacag
    32101 cgaaatccct tcgttctctg caatcaccac agccagcggc tcactccact ggctgctttc
    32161 tttgagatag ccttcggtat ccgtttctat ctctttacct tcgaagatca gcataattat
    32221 tactacctta atcagactgg cggcagtgta acaaaaaaca aagccccgca taaagcgagg
    32281 ctatgaaagt gttagcgggt gagattaatc gcggctagcg aagcccagaa tgctcagcag
    32341 gctgacgaag atgttgtaca gcgaaacata caggctaacc gtggcacgaa tatagttcgt
    32401 ctcaccgcca tgaatgatgt tgctggtttc aaacaagata gcgccagagg agatcagaat
    32461 gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc aggaagatat tagcaaccat
    32521 accaatcagc accaccacaa tacccgccat cagcataccg ccgaggaacg acatatcttt
    32581 gcgggtggtc agcacatatg cagagcagca gaagaacact aacgccgttc cgcccagtgc
    32641 catagcgatt acgtcaccca ttccggcaga cagataggtg ttcagaatag gtccgaggat
    32701 ataacccaga aaaccggtaa aggcgaatgc ggagataatc ccggtcggct tattcgccgt
    32761 tttataggtc aggaacatca aaccatacat acccaccagc gtcagaatca gacccggaga
    32821 tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct
    32881 cagcagaaaa taggtattac gcagcacctt atgggtgcta agcagtgatg tacggtcatg
    32941 tgaagaacta acaatacgat ccattgagtc actctctatg acagatgtaa ttaattaagc
    33001 agcataatga taatgcgtaa gggcacccag aagttttacc catctttacg catttgatct
    33061 ggaacaggtt taacagcgga ttatcaggtc attaagcaaa tataacgccc tgagaatttc
    33121 gacaggcaaa agaaaaaggg gttagcattt agctaacccc ttatcttatt tggcggaagc
    33181 gcagagattc gaactctgga accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa
    33241 ccgctcggcc acacttccgg aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac
    33301 ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc attgatagcg tgaaataaca
    33361 gcagattgat catttcatca ccatgaattc cttctctttt actcgtttag caaccggcta
    33421 aacatcccca ccgcccggcc aaaagaaaaa taggtccatt tttatcgcta aaagataaat
    33481 ccacacagtt tgtattgttt tgtgcaaaag tttcactacg ctttattaac aatactttct
    33541 ggcgacgtgt gccagtgcag aaggatgagc tttcgttttc agcatctcac gtgaagcgat
    33601 ggtttgcctt gctacaggga cgtcgcttgc cgaccataag cgcccggtgt cctgccggtg
    33661 ttgctaggag aagagacgtg cgatatgaat aacgaggaaa cattttacca ggccatgcgg
    33721 cgtcagggcg ttacccggcg cagctttctc aaatattgta gtctggctgc cacgtcgctg
    33781 ggattaggcg cgggaatggc accaaagatt gcctgggcgc tggagaacaa accgcgcatt
    33841 ccggtggtat ggatccacgg tctggaatgc acctgctgta ccgaatcttt tatccgctcc
    33901 gctcacccac tggcgaagga cgtcatcctt tcccttattt ctctcgatta cgacgatact
    33961 ttgatggctg ccgccggaaa ccaggcggaa gaagtctttg aagacatcat cacgcaatac
    34021 aatggcaaat atatcctcgc agtagaaggc aatccgccgc tgggcgagca ggggatgttc
    34081 tgtatcagca gcggtcgacc gtttattgag aaactcaaac gtgccgctgc cggagccagc
    34141 gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg tgcaggccgc gcggcccaat
    34201 ccgacgcagg caacgcctat cgacaaagtc atcaccgaca aacccattat caaagtacct
    34261 ggctgcccgc cgatcccgga tgtgatgagc gccatcatta cttacatggt gacctttgat
    34321 cgcttgccag atgtcgacag aatgggccgt ccactgatgt tttatggtca gcgtatccat
    34381 gataaatgct atcgccgcgc tcacttcgac gccggagagt tcgtccagag ttgggatgat
    34441 gacgctgccc gcaaaggtta ctgcctgtac aaaatgggct gcaaagggcc taccacctat
    34501 aacgcctgtt cctccacacg ctggaatgat ggcgtttctt ttccaatcca gtctggtcac
    34561 ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg gttcgttcta cagccgcgtg
    34621 gtcgatattc cgcaaatggg tactcattcc actgccgata ccgtcggttt aaccgcgctt
    34681 ggcgtggtgg cagcggctgt tggtgtgcac gcagtcgcca gcgccgttga ccagcgcaga
    34741 cgtcataacc agcaacctac agaaaccgaa catcagccag gcaatgagga taaacaggca
    34801 tgagcactca gtacgaaact cagggataca ccatcaataa tgccggacgc cgcctggtgg
    34861 tcgacccgat tacgcgcatc gaaggccaca tgcgctgcga agtgaatatt aacgatcaga
    34921 atgtgatcac caatgccgtc tcctgcggca ccatgtttcg cgggctggag atcattctgc
    34981 aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg tatctgcggc gtctgtactg
    35041 gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc tatcggtatt aaagtgccgg
    35101 acaacgccaa tatcatccgc aacattatgc tggcaacgct ctggtgccac gatcatctgg
    35161 tgcacttcta tcagcttgcc gggatggact ggatcgatgt gttagatgcg ctgaaagccg
    35221 acccgcggaa aacctccgaa ctggcgcaaa gtctctcctc ttggccgaaa tcatcccctg
    35281 gctatttctt cgacgtacaa aaccgcctga aaaaatttgt tgaaggcggg cagttgggga
    35341 tcttccgcaa tggctactgg gggcacccgc agtacaaact gccgccagaa gctaacctga
    35401 tgggctttgc ccactatctc gaagctctcg atttccagcg tgaaattgtc aaaatccacg
    35461 cggtctttgg cggtaaaaac ccgcatccaa actggattgt cggcgggatg ccttgcgcca
    35521 tcaacattga cgaaagcggc gcggtcgggg cagtcaatat ggaacgcctg aacctggtgc
    35581 agtcaattat cacccgcacg gcggacttca ttaacaacgt gatgatcccc gacgccttag
    35641 ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac gggtctttct gataaatgtg
    35701 tcctcagcta cggggcattc ccggatattg ccaacgactt tggtgagaaa agtctgctga
    35761 tgcctggcgg cgcggtgatt aacggcgatt tcaacaatgt gctgccagtg gatttggttg
    35821 atccgcagca ggtgcaggag tttgtcgatc acgcctggta tcgttatccc aacgatcagg
    35881 tcgggcgtca tccgtttgat ggcatcaccg acccgtggta caaccccggc gatgtcaaag
    35941 gcagcgatac caacattcag cagctgaatg aacaggaacg ctactcgtgg atcaaagcgc
    36001 cacgctggcg cggtaacgcg atggaagtgg ggccgctggc gcgcacgtta atcgcttatc
    36061 acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat gatgtcggcg ttgaacctgc
    36121 cgctttccgg tatccagtca acgttaggcc gcattttgtg ccgcgcgcac gaagcgcagt
    36181 gggccgcagg taagttgcag tatttcttcg acaagctgat gaccaacctg aaaaacggca
    36241 atctcgccac tgcttccacg gaaaaatggg aacctgcaac ctggccgaca gagtgccgtg
    36301 gtgtcggttt taccgaagcg ccgcgcgggg cgttaggcca ctgggccgcc attcgcgatg
    36361 gcaagattga tctctaccag tgcgtggtgc cgaccacctg gaacgccagc ccgcgcgatc
    36421 ccaaagggca gattggcgct tatgaagcgg cgctgatgaa caccaaaatg gcgatccccg
    36481 agcaaccgct ggagatcctg cgtactctgc acagctttga cccgtgcctc gcctgttcaa
    36541 cacacgtgct gggcgacgac ggtagcgagc tgatctccgt gcaggtgcgt taacagcgaa
    36601 ggagaatcat catgcaacag aaaagcgaca acgttgtcag ccactatgtc tttgaagcgc
    36661 cagtccgcat ctggcactgg ttgacggtgt tatgcatggc ggtgttgatg gtcaccggat
    36721 actttatcgg caagccgcta ccttccgtca gcggcgaggc gacgtatctg ttctatatgg
    36781 gctacatcag gttaattcac ttcagcgccg ggatggtttt taccgtggtt ttgctgatgc
    36841 ggatctactg ggcttttgtt ggcaatcgat actcccgcga gctgtttatc gtgccggtat
    36901 ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg ctggtatctg tttctggcaa
    36961 aacgtccgag tgccgatata ggccataatc ccatcgccca ggcggcgatg ttcggctatt
    37021 tcctgatgtc ggtctttatg atcatcactg gttttgcgct gtacagcgaa cacagccagt
    37081 acgctatttt tgcgccgttc cgttatgtgg tggaattttt ctactggacg ggtggcaact
    37141 caatggacat tcacagctgg catcggctgg ggatgtggct gattggcgcg tttgtgatcg
    37201 gtcatgtcta catggcgctg cgtgaagaca tcatgtccga cgacacggtg atctccacca
    37261 tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag taacaaggag cgttcatgag
    37321 cgagcaacgc gtggtggtca tggggctggg caacctgctg tgggccgatg aaggcttcgg
    37381 cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg cccgagtatg tggagattgt
    37441 cgatggcggt actcagggac tgaacttgct ggggtatgtc gaaagcgcca gccatctgtt
    37501 gattctcgat gccattgact acgggctgga acctggaacg ctgcgaacct atgccggaga
    37561 acgcattccg gcttatctca gcgcgaagaa aatgagcctg catcagaaca gtttctccga
    37621 agtgttggcg ctggcggata tccgcggaca tctgccagca catattgccc tcgtcggtct
    37681 gcaacccgca atgctcgacg actacggcgg tagcctgagc gaactggcac gggagcaact
    37741 gcccgctgcg gaacaggcgg cgctggcgca gctggctgcg tggggaattg tgccgcaacc
    37801 ggctaatgaa tcgcgctgtc tcaattatga ctgtctgtcg atggaaaatt acgaaggcgt
    37861 tcgcttgcgc cagtaccgga tgacacagga ggagcaggga tgagcaacga cacgccattt
    37921 gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc cagtcagtga atcccgtctt
    37981 gacgactggc ttacgcaagc gccagacggc gtggtgttat taagcagtga cccgaaacgc
    38041 acgccagagg tcagcgataa tccggtaatg attggcgaat tactgcacga gtttcccgac
    38101 tatacatggc aggtggcgat tgctgacctt gagcagagcg aagccatcgg cgatcgcttt
    38161 ggcgtctttc gctttcctgc cactttagtg tttaccggcg gaaactatcg cggcgtgctg
    38221 aatggtattc acccgtgggc ggaactgata aacctgatgc gcgggcttgt cgaaccgcag
    38281 caggagcgtg cctcatgagc gaaacttttt tccatctgct ggggccagga acgcaaccga
    38341 acgatgacag tttcagcatg aatccactgc cgatcacctg tcaggtgaat gatgaaccga
    38401 gtatggcggc cctggagcaa tgtgctcaca gcccgcaggt gattgcgctg ttaaacgagt
    38461 tacaacatca actaagcgaa cgccaaccgc cgttgggcga ggtgctggca gtcgatctgt
    38521 taaatctcaa cgccgacgat cgtcacttta tcaatacgct tctcggggaa ggggaagtgt
    38581 cagtgcgcat tcagcaggct gacgacagtg aaagtgaaat acaggaggcg atcttctgcg
    38641 gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct ggaggacaaa ctggaggctg
    38701 gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa tctcttgccg acagattcgc
    38761 tgttaccgcc gcccattgat ggcctgatga atggcctacc gttggcgcat gagttactgg
    38821 cacatgtacg taaccccgac gcgcagccgc acagcattaa tctgacgcaa ttacccatca
    38881 gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc gggaaatatt cagattcgta
    38941 ccattggcta tggcgagagc tatatcaacg ccacggggtt acgccatgtc tggcatttac
    39001 gctgtacgga caccttaaaa ggcccgttac tggaaagtta tgaaatctgc ccaataccgg
    39061 aagtggtgct ggcagcgcca gaagatttgg tcgactctgc gcagcggctt agcgaggtat
    39121 gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg taagtatcgc tttcagtctt
    39181 atgaatatcg caatcggcga atacctctgg tcgtagagtt tcaggataaa gagggagatc
    39241 taccattatc gggttatttt tctctcttcg cctacaggag tgcgcatgtg ggatgtcatt
    39301 gatttatcgc gctggcagtt tgctctgacc gcgctgtatc actttttatt tgtacccctt
    39361 accctggggc tgattttttt gctggctatc atggaaacca tttacgtggt caccggcaaa
    39421 acaatctacc gcgatatgac gcgcttctgg ggtaagctct tcggtatcaa ttttgctctt
    39481 ggcgtggcta ccggcctgac catggagttt cagtttggta ctaactggtc attctattcc
    39541 aactatgtgg gcgatatttt tggcgcacca ctggcgatgg aagcattaat ggccttcttc
    39601 ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc aacggctgaa taaataccag
    39661 cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc tctctgcgtt gtggatattg
    39721 aatgccaatg gttggatgca atacccgacc ggtgcgcatt ttgatatcga caccctgcgt
    39781 atggagatga ccagcttcag cgagctggtc tttaatccgg tcagccaggt gaaatttgtg
    39841 cacaccgtaa tggcgggcta cgtgaccggg gccatgttta ttatggcgat cagcgcctgg
    39901 tatttactgc gcggacggga gcgcgatgtc gcattacgct cgtttgccat cggttccgtc
    39961 ttcggtactc tggcgattat cggtaccctg caactcggag acagttctgc gtatgaagtc
    40021 gcgcaagtac aaccggtaaa actggcggcg atggaagggg agtggcaaac ggaacctgca
    40081 cctgcaccgt tccatgtggt tgcctggccg gaacaggatc aagagcgtaa cgcctttgcc
    40141 ctcaaaattc ccgcgctgct agggatcctc gccactcact cattagataa acccgtgccg
    40201 ggtctgaaga atttgatggc tgaaacctac ccacgcttgc aacgcggacg tatggcctgg
    40261 ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc atgtgttgca ggcattccgg
    40321 gaactggaag gtgacctggg ctacggcatg ttgctctccc gctatgcgcc ggatatgaat
    40381 catgtcacag ccgcacagta ccaggcggcg atgcgtggcg cgatacctca ggttgcgccg
    40441 gtattctgga gtttccgcat catggtgggc tgtggttccc tgctgctact ggtgatgctg
    40501 attgcgcttg tccagacgct gcgtggcaaa atcgaccagc atcgctgggt gctgaaaatg
    40561 gcgctctgga gtttgccgtt gccgtggatt gcgattgaag ccgggtggtt tatgaccgag
    40621 tttggtcgtc agccgtgggc gatacaggac atcttaccga catactccgc gcactccgct
    40681 ttaaccacag gacaactggc tttctcactg atcatgatcg tagggcttta caccctgttc
    40741 ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc tggggccgag cgcgatgcag
    40801 agtgaacaac cgacgcagca acaggggtaa aggagaaaat catgtttgat tatgaaacat
    40861 tgcgcttcat ctggtggctg ctgattggcg tgatcctggt ggtctttatg atctccgacg
    40921 gatttgacat ggggattggc tgtttgctgc cgctggtggc gcgtgatgat gatgaacgcc
    40981 ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa tcaggtctgg ttgatcctcg
    41041 caggtggggc attatttgcc gcctggccca gagtgtatgc agcggcgttt tccggctttt
    41101 atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg cccgctggcc tttgattatc
    41161 gcggaaaaat cgccgatgca cgctggcgta aaatgtggga tgccggtctg gtcatcggca
    41221 gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa cttgttgctc ggcgtgccgt
    41281 ttgccttcac accgcaatta cgcgtggagt atctcggcag cttctggcaa ctgctgacgc
    41341 cattcccttt attgtgcgga ttgctcagcc ttgggatggt gattttgcaa ggtggcgtct
    41401 ggttacaact gaaaactgtt ggtgtgattc atctgcgttc acagctggcg actaaacgcg
    41461 ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta ctggctgtgg gtcggtattg
    41521 atggctttgt actgctcgcc caggatgcta acggtccttc caatccgtta atgaaactgg
    41581 tggcagtgct acctggtgcc tggatgaata attttgtcga gtcgcccgtt ttgtggatct
    41641 tcccgctgct ggggttcttc tgcccattgc tgacggtgat ggcgatttat cgtggtcgcc
    41701 cgggttgggg atttttgatg gcgtcattga tgcaatttgg cgtgattttc acggcaggca
    41761 tcacgctgtt cccctttgtc atgccgtcaa gcgtgagtcc aatctccagc ctgacgttgt
    41821 gggacagcac ttccagtcag ctgacgctga gcattatgtt ggtaatcgtg ctgatatttt
    41881 tgcctattgt gttgctctac actctctgga gctactacaa aatgtgggga cgcatgacga
    41941 cagaaactct ccgccgtaac gaaaacgagc tgtactaagg aacaggaaca atgtggtatt
    42001 tactttggtt cgtcggcatt ttgttgatgt gttcgctctc cacccttgtg ttggtatggc
    42061 tggacccgcg tctgaaaagt taacgaacgt aggcctgatg cggcgcatta gcatcgcatc
    42121 aggcaatcaa taatgtcaga tatgaaaagc ggaaacatat cgatgaaagc gatcttaatc
    42181 ccatttttat ctcttctgat tccgttaacc ccgcaatctg cattcgctca gagtgagccg
    42241 gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg gtgtgcgtgc tccaaccaag
    42301 gccacgcaac tgatgcagga tgtcacccca gacgcatggc caacctggcc ggtaaaactg
    42361 ggttggctga caccgcgcgg tggtgagcta atcgcctatc tcggacatta ccaacgccag
    42421 cgtctggtag ccgacggatt gctggcgaaa aagggctgcc cgcagtctgg tcaggtcgcg
    42481 attattgctg atgtcgacga gcgtacccgt aaaacaggcg aagccttcgc cgccgggctg
    42541 gcacctgact gtgcaataac cgtacatacc caggcagata cgtccagtcc cgatccgtta
    42601 tttaatcctc taaaaactgg cgtttgccaa ctggataacg cgaacgtgac tgacgcgatc
    42661 ctcagcaggg caggaggggc aattgctgac tttaccgggc atcggcaaac ggcgtttcgc
    42721 gaactggaac gggtgcttaa ttttccgcaa tcaaacttgt gccttaaacg tgagaaacag
    42781 gacgaaagct gttcattaac gcaggcatta ccatcggaac tcaagatgag cgccgacaat
    42841 gtctcattaa ccggtgcggt aagcctcgca tcaatgctga cggagatatt tctcctgcaa
    42901 caagcacagg gaatgccgga gccggggtgg ggaaggatca ccgattcaca ccagtggaac
    42961 accttgctaa gtttgcataa cgcgcaattt tatttgctac aacgcacgcc agaggttgcc
    43021 cgcagccgcg ccaccccgtt attagatttg atcaagacag cgttgacgcc ccatccaccg
    43081 caaaaacagg cgtatggtgt gacattaccc acttcagtgc tgtttatcgc cggacacgat
    43141 actaatctgg caaatctcgg cggcgcactg gagctcaact ggacgcttcc cggtcagccg
    43201 gataacacgc cgccaggtgg tgaactggtg tttgaacgct ggcgtcggct tagcgataac
    43261 agccagtgga ttcaggtttc gctggtcttc cagactttac agcagatgcg tgataaaacg
    43321 ccgctatcat taaatacgcc gcccggagag gtgaaactga ccctggcagg atgtgaagag
    43381 cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc aaatcgtgaa tgaagcacgc
    43441 ataccggcgt gcagtttgta atgcataaaa aagagcattc agttacctga atgctctgag
    43501 gctgatgaca aacgaagaac tgtctaatgc gtagaccgga agaggcgttc acgccgcatc
    43561 cggtcacttt accttcagtt ttactctttc tcggagtaac tataaccgta atagttatag
    43621 ccgtaactgt aagcggtgct ggcgcgttta atcacaccat tgaggattgc gcctttaata
    43681 ttaacgccag cctgttccag acgctgcatt gacaagctca cctctttggc ggtgttcaag
    43741 ccaaaacgcg caaccagcag gctggtgccg acagagcgtc ccacgaccgc agcatcgctc
    43801 accgccagca tcggcggcgt atcgacaatc accagatcgt aatggtcgtt cgcccattcc
    43861 agtaattgac gcatccgatc gcgcatcagc agttcagacg ggttaggtgg cacctgaccg
    43921 cgagtaatca catcaaagcc tcctttgcca aaatgctgga tcactttgtt gagctcatct
    43981 ttacctgcca gatattccga caagccatgt tcattactca cggtaaacag gttatgcgaa
    44041 taaccacggc gtaagtcggc atcaataaat aacacttttt gatcggactg ggcgatcacc
    44101 gctgccagag ttgaactgac aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc
    44161 agaatgttat tctccgtctc catcatagcg aagtgcagac tggttcgtag cgcacgtacg
    44221 gcttccacag cagaatccgc cgggttatcc accgccagga aggggatatt tttagtacga
    44281 tggcgctgct gattagaaaa taaatttttc ttacgcagac gggtgcgttt atccagccac
    44341 tcggacattg ggatagtggc ataaacgttg atgccgtgct cttccagttg ttccggtgct
    44401 tctacaccac gacgcaacat cgctcgcgcc agcacggcac ccaccgaaat aaacaggcca
    44461 agaataaaac caagcaccac gttcaacgct tttttcggtt tcactggctg cggctgagtg
    44521 actgccgggt cgataatccg cacgttacca atggcactgg atttcgaaat actcaactcc
    44581 tgctggcggt taagtaattg cagatatacc gcacggcccg cttctacgtc acgacttaaa
    44641 cgcaacactt cctgttgggt ggaaggcata gccgataccc gcttattcag gcgtttgcgt
    44701 tcttgctcca gcgtctggcg tttttccagc agcgcacgat aagttgggtg atctttctta
    44761 tacagctggg agatctctgc ctcgcggaaa gtcagctcat tgagttgatt atcaacgttc
    44821 acaatctgct caagaacggc tttggcttcc aggttaaggt caaccgaatc gcgctgctgg
    44881 cgataaacgt tgagtttttc ttccgcctgg tccagctcgc tgcgcacttc aggcaattgg
    44941 cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc
    45001 aaatagttgt tagcgatgct gttcagaata cgagtaatca actggggatc atcaccggtc
    45061 atggtaagtt ccagcatccc actttcttta ctgcgttcgc taacggtaaa ggtttcctgc
    45121 aacgcgttaa tcgcttccag ttcggtacgc tggctcagga caaactgtgt tcctggtttg
    45181 gccttaatat ccgcgatagt cagcgcaacg ccatcttttt ccagacgctg gccgaccata
    45241 ccattgacgg tgaactcttc accttccagc gtatagtggc cgttttcccc aaccgtgagt
    45301 gtcagttgct gatcctgacc attcagttgt ggaatatgca tccagctgat cgccagctca
    45361 cctggtttct ctttggttaa tctcgcccag ccgcgaccca caatcggaaa atacttctgc
    45421 tcaaccatgt cgcgcagatt cagttcagca atggttttac cgagaatcat gcgcgattgc
    45481 agcagttgga tctccggtgc agactcgggc gatgagttag ggatcatatc gctcaggcca
    45541 ctgagaatgg cgttgccctg tttctgctca acctggacca gagtatctgc ctgataaatt
    45601 ggcgtgctta acagcgagta agcgacagcg atcagcgtga ataacgcggt cacgctgata
    45661 ataaacttac ggtgatccca taactcgccg accagacgaa gcagatcgat ctcattttcc
    45721 tgagtgctgc ctggtggcgt attcatattt ttagttgtca tcgtatttta taaactcaaa
    45781 ttaccggctg aggcgcttcg cccattcctg actggcgcgc tccaacatac cgtagacatg
    45841 ttcaaatgcg tcctgacttt tacgataggg atccgggatc tctttctgtt ccagccattg
    45901 cccaaacagc attgtcttcc cgcggacctc gggagcgatt gcggtaacct gagcaatatg
    45961 ttccgactcc attgccagaa tcagatcgta atttcgtgcc atctcagcag tgagcttacg
    46021 tccggcatgt ccttcaagag atacgccatg attagcggca acatctgccg ctgtcgcatc
    46081 tgcagggtgt tttaccagac catgaacacc tgccgatttg acttttacgc ccggcagacg
    46141 tttacgcagc aagcgctcgc caatgggcga acggcaaatg ttgccggtac aaaccaccag
    46201 gattgagtta aattttagtt gggccatgtt tgaatccacc gtgaagtctc tgtcaggtca
    46261 tgcaccccgg aaatcgtcgg taccagcaga gaaatcacac ggttccaacg agccagcggc
    46321 gcggtagtga cataaacaat gtcgtaaggt tcaagctgga attccgttcc gagtatcatt
    46381 gccgatgcgt ccttcgcatt cagctgataa atattggcga ttttgccatt ctgcttgttt
    46441 tgcgttgcac gaataacaaa aatccccgtc gcatcagcga catcctgatt cataccctca
    46501 gcgttaccca gcgcttccgc cagggtcata ccgctgcggt ccattttcag cgtgctctgt
    46561 ttacccactt cacccatcac gaagactttc agatcatcgt tgcgcggaat aaataaaata
    46621 tcgcccgggt gcagcagttt gttctggcgc aagtcgccac gctgcatcag cgcatagagg
    46681 ttcactttgg tctttacgcc attctgggtg agcaccacgt tgcgccagtc tgcatctgcg
    46741 gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca gaggaatatt ggtaatcggc
    46801 tgctgacccg atttgctgac ttcgccggtg acgtacgctt tttgcgatcg gaacgccgcg
    46861 acgctgacat cgacttgcgg gctttcaatg actgaatcga gacgcgctgt aatctcgttg
    46921 cgtacctggg taagtgtttt cccggccact ttcaggcgac caatataggg gtagaaaatc
    46981 gcaccatcgg cgttaaccca gttgccggta tcactggcgc tacggtattg ccctgcgggg
    47041 gtggttagtt ccgggtgatc ccagaccgta accatcaaca catcaccaat cccgatacga
    47101 tattcatagt ttgcgatttg ctgctccagc tccgggttag cctgcgacat aatcggtttg
    47161 gcgcgcaact gatcaatcaa tcctggcgtg acgggataga tattgaccat tttgtccagg
    47221 tcatactgat tgtcaggtag ttcaatgaca tccttgttac tggtcgataa attctgacca
    47281 ggaacaaggg tacatgctgt caaagacaga acagccaatg tcagtaccga aaacttaaaa
    47341 atgttctttt tcattggttt atatttatca gtcaatgtaa tgttatgaat tacgtcaggt
    47401 tattgtccaa ccggtacttc tcgctctgcc gtcattggat agagctggta tttgcgtgac
    47461 agcatttgtc caccatcacg cgtcaatggt gtccacgaaa ccaccgcgcg gttgcggtta
    47521 ggtccaatgg tcatcaagtc gaatggaatc gagatataaa aacctttact aaagccgcct
    47581 tcgccgtagt catctttaga tacgttactg attgccgccc atacccctac cgccacgccg
    47641 ctgtcaaagc gtttggcgac gtcgatcgtt gcgcctttat cttttgccag atattgccca
    47701 acgctaagtt tcatcagtac gccattgagc gtcggcgggt tccagtaagc cgtcacgaaa
    47761 cctgttggcg tggaataatc ggtgaaacgc atcatgttgt cccagtcacg ctgcttcacg
    47821 tagttaacgt ccacacccag cgcccagctg gcatctagcg ggcgataaag cagctcggaa
    47881 ccgacaccgg catacatcgt ttccagataa ccgccataca cctggccata gaaaccattg
    47941 cctaagtcag caaaatagtt cgcctgcaag ttgttgagat aaacgtcatt gcgaacgtag
    48001 tcacggatat gcgtgcgcac gcggggcagg gtagagtccg cgggcaacag cgaagactta
    48061 aacttgtcgt agttgttgta aatattggtg aaaataccgc catcaagcag caggtggtcg
    48121 gtaaaccagt agcgggcact ggacatcagc cccagctgga acatatagaa atcttccggg
    48181 ccgcccagcg actgtgaaag tgttgggttg aaagagtagc taaagcgatc ttcacgaata
    48241 cggtagcccc gaccaaaggc agaaagatct tctgcttcaa cacgttgttg ttgcagttgc
    48301 tctgattgac caggcgctgt accagccagc tgtttgcgca ggctggctac gtcggtttcg
    48361 gtagtcacca tcgccatatg ctcgcgcttt tgcgtcacgc taatcttctc aacgccttgc
    48421 ggcaggttat tcaccagaat ccggttggca cgatcgaccg cttcacgaga gtcacggtat
    48481 ttgtattgct gaccagacat atacagcgtc ttatcgcgta gctgaatttc tggcgcgtca
    48541 aagcccgcgt tatacttcag ggcggtaagt tggtttgcta ccgtggtgta ctgcaatcct
    48601 tcagattcag gcgcaggttg atatgccggt tttggcgtat cgcgcagggc agggcgcaga
    48661 tcgttgaaat tggtccgtaa agtgaaacca aacatcaacg tgttaccgcg ttcataactc
    48721 aggttgagat ctgcccagct ggcagcgcga taaactgcac cgacgttgaa atggcttgcc
    48781 tgaggcagtt tgccagcgaa atcattctgg taattgttgc cgtcgtattc gagtttcaga
    48841 cgcaggggat tccacggcgt ttgatactca atgccgccaa agatggaagc cgggccacga
    48901 aagatatcgc taaagctgat atcgcccgca tcgtgagact ctgcgcgatg acaatattta
    48961 tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat taccggcgta gccccatgcc
    49021 atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg ccaccagata ctcaccgtca
    49081 aacagacccg taccagcaat atcacgttta ccaaacgcca cttgcggtag ccagtgacct
    49141 tcttcccaca ggcgaagttt aaaatcgaat gatttgtctt tgtatgactg atcgccgctg
    49201 aaatcttccc actggctata tttgcgtgtg cgcacatccg tataacgaat ggtgccttcc
    49261 agccatggga acagcgccac ggaggtggag tagaaccggt attgatcgtt atcccggtag
    49321 ttgacgctga attcaccttc cggtgcgatg cgcgcatttg gcatctgcaa caatcctgtg
    49381 ccgccaaagt ctgattgcga cgaacccagc ggatccgggt aggttaatac ttcagcatga
    49441 caggcactgg agacggcaat ggccaggcag cttaaaagat aagaattctt cttcatcagt
    49501 caggaacacg ttgcgttaaa acagacacaa tctggtcatt taaatcagca tatttttcgg
    49561 gcagtacatg cgcagaaaag cccaaccaaa gctggctgcc aggcggtggt tccacatgtc
    49621 ttttattcca cagggcaaca ggggcgacga cagtctcacc ctcaggcgta atcaccataa
    49681 cgttattctt gtccgcgcct gcaaggcggg ggtgatcttg cagataatcg gtaacgctac
    49741 ggcctgcctg ccagggcaat tgcccggccc cagaaaccgc acccagcaga gtaatagtca
    49801 ccggacgctg tacggtatag agcgtatagt ccccgaccaa cgggggattg ctgttctcat
    49861 ccactcgtac aaaatcaggg tccagtttga ccggtaagcg accagtgata ttgagattga
    49921 gtaactgctg gcgcacggat ttaatcgttg ccgccacatc gtcgtcagct tctgcttccc
    49981 aggaagcaag ctgtgccatg acatgttggt agtctttaag cgctttagct ttcgccgcag
    50041 agtcggtcag caaggcacca ggccaccaga ggcgatcgcg cagttgcggt tgtgtcacca
    50101 actggacaac attttccacg gggccaacgg aaagtgtctg ttgttcgcca ggcagataaa
    50161 tagtcaccgt tccttgcgca aaggcatggg gtgtcattac gtaaagtacg ctggcaatga
    50221 aatacgactg taatttattc atggtgcggg tttaagaaac gtcatttcta cgggaataac
    50281 ccctgcgcct aacatttgcc gactttgacg aacctggcca gtagccgaat cgacccagaa
    50341 tgtgttttgc cactgttttt ctggccgcgt ggaagtcacc tcttcctgcc agacattaca
    50401 cagcacattg cgacccgcaa tattcagcgt ctctaatcca gcaaaggaaa aagtagaact
    50461 cagggtcgca gagcgaaaat ggttgtcttc actccagaga atatcgcgcg tccagcggct
    50521 gccatctttt attgccagcg cgttacgcag agggtcctgc ccggaatttg tcacttccag
    50581 cagattatta ttaagtttga cggtttttaa aagacgtcca ttgtgggtaa ccagcatggc
    50641 gttatcctgg gataaccatt tgctttgttc ttgttctata tatcccagta ccacaaagat
    50701 tcgttgccct tcattaaggc gtaaatacat cgtggaataa ggcaacgcct ggatctgctg
    50761 atccgctacg gtgatatcct gattatcgaa aaggctggcg cgaaatgtat cgaccatact
    50821 ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa atcgatagaa taagagggcg
    50881 cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat ctctaaagcc cggtacgaat
    50941 atcgcaaccg ggtatcattt aactttaatt tcgacgcgtt aaagcagtat tagcgtgtag
    51001 tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc actgtcattt gctgcaacaa
    51061 ctgccaacgt cgccacacca acagccgttg ccacaccgac aaccgtcgcc gtgccgacag
    51121 cgccagcagc cacgccacca gtggtagccg cagtcgtggt tgcaccggta gtggtcgttg
    51181 tggtgccagt agtacccgtc gttgcctcgg tcccgtttgc tgcttcaggg gcagcaaatg
    51241 cagctggcgt cgtcaacatg aaggccataa ggattgcaga aag
//
LOCUS       DAPHJE010000015        49762 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPHJE010000015 DAPHJE010000000
VERSION     DAPHJE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.015"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            166..837
                     /locus_tag="RH222_000926"
     CDS             166..837
                     /locus_tag="RH222_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDV3795984.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            847..2043
                     /locus_tag="RH222_000927"
     CDS             847..2043
                     /locus_tag="RH222_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV3795985.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYSQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2021..2602
                     /locus_tag="RH222_000928"
     CDS             2021..2602
                     /locus_tag="RH222_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV3795986.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2604..3377
                     /locus_tag="RH222_000929"
     CDS             2604..3377
                     /locus_tag="RH222_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDV3795987.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3562..3837
                     /gene="frmR"
                     /locus_tag="RH222_000930"
     CDS             3562..3837
                     /gene="frmR"
                     /locus_tag="RH222_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV3795988.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3872..4981
                     /gene="frmA"
                     /locus_tag="RH222_000931"
     CDS             3872..4981
                     /gene="frmA"
                     /locus_tag="RH222_000931"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV3795989.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5074..5907
                     /gene="fghA"
                     /locus_tag="RH222_000932"
     CDS             5074..5907
                     /gene="fghA"
                     /locus_tag="RH222_000932"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV3795990.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6032..6571)
                     /locus_tag="RH222_000933"
     CDS             complement(6032..6571)
                     /locus_tag="RH222_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV3795991.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTEGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6673..7884)
                     /gene="mhpT"
                     /locus_tag="RH222_000934"
     CDS             complement(6673..7884)
                     /gene="mhpT"
                     /locus_tag="RH222_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV3795992.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8060..9073)
                     /gene="dmpG"
                     /locus_tag="RH222_000935"
     CDS             complement(8060..9073)
                     /gene="dmpG"
                     /locus_tag="RH222_000935"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV3795993.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9070..10020)
                     /gene="mhpF"
                     /locus_tag="RH222_000936"
     CDS             complement(9070..10020)
                     /gene="mhpF"
                     /locus_tag="RH222_000936"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV3795994.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPKSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVKLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10017..10826)
                     /gene="mhpD"
                     /locus_tag="RH222_000937"
     CDS             complement(10017..10826)
                     /gene="mhpD"
                     /locus_tag="RH222_000937"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV3795995.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10836..11702)
                     /gene="mhpC"
                     /locus_tag="RH222_000938"
     CDS             complement(10836..11702)
                     /gene="mhpC"
                     /locus_tag="RH222_000938"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV3795996.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11720..12664)
                     /gene="mhpB"
                     /locus_tag="RH222_000939"
     CDS             complement(11720..12664)
                     /gene="mhpB"
                     /locus_tag="RH222_000939"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV3795997.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12666..14330)
                     /locus_tag="RH222_000940"
     CDS             complement(12666..14330)
                     /locus_tag="RH222_000940"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV3795998.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTTGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14407..15354
                     /gene="mhpR"
                     /locus_tag="RH222_000941"
     CDS             14407..15354
                     /gene="mhpR"
                     /locus_tag="RH222_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV3795999.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15431..16513
                     /gene="lacI"
                     /locus_tag="RH222_000942"
     CDS             15431..16513
                     /gene="lacI"
                     /locus_tag="RH222_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV3796000.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16636..19710
                     /gene="lacZ"
                     /locus_tag="RH222_000943"
     CDS             16636..19710
                     /gene="lacZ"
                     /locus_tag="RH222_000943"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV3796001.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19762..21015
                     /gene="lacY"
                     /locus_tag="RH222_000944"
     CDS             19762..21015
                     /gene="lacY"
                     /locus_tag="RH222_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV3796002.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21081..21692
                     /gene="lacA"
                     /locus_tag="RH222_000945"
     CDS             21081..21692
                     /gene="lacA"
                     /locus_tag="RH222_000945"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV3796003.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLLKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(21795..22976)
                     /gene="cynX"
                     /locus_tag="RH222_000946"
     CDS             complement(21795..22976)
                     /gene="cynX"
                     /locus_tag="RH222_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV3796004.1"
                     /translation="MRNHQKTFTILLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(22982..23452)
                     /gene="cynS"
                     /locus_tag="RH222_000947"
     CDS             complement(22982..23452)
                     /gene="cynS"
                     /locus_tag="RH222_000947"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV3796005.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23483..24142)
                     /gene="cynT"
                     /locus_tag="RH222_000948"
     CDS             complement(23483..24142)
                     /gene="cynT"
                     /locus_tag="RH222_000948"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV3796006.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24252..25151
                     /gene="cynR"
                     /locus_tag="RH222_000949"
     CDS             24252..25151
                     /gene="cynR"
                     /locus_tag="RH222_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV3796007.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25191..26474)
                     /gene="codA"
                     /locus_tag="RH222_000950"
     CDS             complement(25191..26474)
                     /gene="codA"
                     /locus_tag="RH222_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV3796008.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26464..27723)
                     /gene="codB"
                     /locus_tag="RH222_000951"
     CDS             complement(26464..27723)
                     /gene="codB"
                     /locus_tag="RH222_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV3796009.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(28055..29941)
                     /gene="prpE"
                     /locus_tag="RH222_000952"
     CDS             complement(28055..29941)
                     /gene="prpE"
                     /locus_tag="RH222_000952"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV3796010.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29981..31432)
                     /gene="prpD"
                     /locus_tag="RH222_000953"
     CDS             complement(29981..31432)
                     /gene="prpD"
                     /locus_tag="RH222_000953"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV3796011.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31466..32635)
                     /gene="prpC"
                     /locus_tag="RH222_000954"
     CDS             complement(31466..32635)
                     /gene="prpC"
                     /locus_tag="RH222_000954"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV3796012.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32680..33570)
                     /gene="prpB"
                     /locus_tag="RH222_000955"
     CDS             complement(32680..33570)
                     /gene="prpB"
                     /locus_tag="RH222_000955"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV3796013.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33808..35394
                     /gene="prpR"
                     /locus_tag="RH222_000956"
     CDS             33808..35394
                     /gene="prpR"
                     /locus_tag="RH222_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV3796014.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35492..35767)
                     /locus_tag="RH222_000957"
     CDS             complement(35492..35767)
                     /locus_tag="RH222_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3796015.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35914..36585
                     /locus_tag="RH222_000958"
     CDS             35914..36585
                     /locus_tag="RH222_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV3796016.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36602..36847)
                     /locus_tag="RH222_000959"
     CDS             complement(36602..36847)
                     /locus_tag="RH222_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796017.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(37259..38074)
                     /locus_tag="RH222_000960"
     CDS             complement(37259..38074)
                     /locus_tag="RH222_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796018.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38317..39366)
                     /gene="yahK"
                     /locus_tag="RH222_000961"
     CDS             complement(38317..39366)
                     /gene="yahK"
                     /locus_tag="RH222_000961"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV3796019.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39743..41125)
                     /locus_tag="RH222_000962"
     CDS             complement(39743..41125)
                     /locus_tag="RH222_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV3796020.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(41135..42085)
                     /locus_tag="RH222_000963"
     CDS             complement(41135..42085)
                     /locus_tag="RH222_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV3796021.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(42228..43646)
                     /locus_tag="RH222_000964"
     CDS             complement(42228..43646)
                     /locus_tag="RH222_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV3796022.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(43646..45193)
                     /gene="fdrA"
                     /locus_tag="RH222_000965"
     CDS             complement(43646..45193)
                     /gene="fdrA"
                     /locus_tag="RH222_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV3796023.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(45183..46046)
                     /locus_tag="RH222_000966"
     CDS             complement(45183..46046)
                     /locus_tag="RH222_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV3796024.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDEQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALVLFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHSFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(46086..46691)
                     /locus_tag="RH222_000967"
     CDS             complement(46086..46691)
                     /locus_tag="RH222_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV3796025.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDLTCLNPFLMSCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            46949..47446
                     /locus_tag="RH222_000968"
     CDS             46949..47446
                     /locus_tag="RH222_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV3796026.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            47538..48470
                     /locus_tag="RH222_000969"
     CDS             47538..48470
                     /locus_tag="RH222_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3796027.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(48512..49600)
                     /gene="pdeL"
                     /locus_tag="RH222_000970"
     CDS             complement(48512..49600)
                     /gene="pdeL"
                     /locus_tag="RH222_000970"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDV3796028.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
BASE COUNT        11659 a        13439 c        12744 g        11920 t
ORIGIN      
        1 tgcatagaaa cattacccag tcgaacctcc gggtctgaaa gcccttgctt ttcagttatt
       61 agcaacattt aacatttata aattaacatc tggctgcgcc agagattacg tctctgagtg
      121 catcgttgtg cttgtagtat aataaataaa atggagtgtc attaagtgga taaggtttta
      181 gattcagccc tcctttcttc ggcaaataaa agaaagggta tccttgctat tggcgcacat
      241 cctgacgata tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca aaaaggaatt
      301 tatatcgcag ccgtggtgat gactaccggc aactctggca cagatggaat aatcgatcgc
      361 catgaagaat cgcgcaacgc cctaaagata ttagggtgcc accaaactat tcatcttaat
      421 tttgctgaca cccgcgctca tttacagctc aatgatatga tttccgccct ggaagacatc
      481 attaaaaatc aaattccttc tgatgttgaa atcatgcggg tatataccat gcatgatgcc
      541 gaccgccatc aggatcatct cgctgtttat caagcttcaa tggttgcctg ccgcactatt
      601 ccacaaattc tcggctacga aaccccaagt acctggcttt catttatgcc tcaggttttt
      661 gaatccgtta aagaagaata tttcacagtc aaacttgctg cattaaaaaa acataaaagc
      721 caggaacgac gcgattatat gcgtcatgat cgcctgcgtg cagttgcaca atttcgcggg
      781 caacaggtca atagcgatct gggtgaaggc tttgtaattc ataaaatgat tctttgagca
      841 aattgcatga aaacctggat atttatctgt atgtccatag caatgttgct atggttttta
      901 agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat tatacctgcg
      961 tataacgaag gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa cccttatttt
     1021 tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt catggcggaa
     1081 gtcaaacgca aatggggcga ccgctttgtt gccgtcacgc aaaaaaatac cggtaaaggt
     1141 ggtgcgctga tgaatggcct caattatgcc acctgcgacc aggttttttt aagtgatgcc
     1201 gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcaga aattgagcgc
     1261 ggtgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg gctgttaccg
     1321 cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct acagcagctc
     1381 ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgcac tgatgtattg
     1441 cgtaagttcg gtttctcaga tcgtactaaa gtcgaagacc ttgatctcac ctggacatta
     1501 gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtatactc acaggaatgc
     1561 aacagcccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggata cgcggtctgt
     1621 atgcgcctgc ataaaagact tttatttagc cgcttcggta tcttcagtat atttcctatg
     1681 ctgttggttg tgatttatgg cgttgggatt tatctcacta cctggtttaa tgaattcatc
     1741 accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctggat cggcgtagtt
     1801 tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt gcctttagcg
     1861 ccgctttccg ttgtgtatgt attattagct tatgccatct ggattattta tggacttatt
     1921 gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc cgcactggtg
     1981 gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact atctgaagct
     2041 taatgttaaa gagaaaaata ttcagattgc agaccaggta attattgatg aaactgcagg
     2101 tgaagttgtt atcggcgcga atacccgtat ttgtcatggt gccgttattc agggtccggt
     2161 agtgattggc gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaataat
     2221 cagcaatggt gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga
     2281 agcaacgatt ggtccgcaat gttttattgc cgactcggta gttgcaaacc aggcatattt
     2341 gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt ctgttcgaac
     2401 tccagaggga attatcgcta ccggatgcga taaattaggt tgttatatcg ggcagcgttc
     2461 acgccttggc gtacaagtta taattttgcc tgggcgaatt atttccccga acacgcaact
     2521 tggcccgcgc gtgattgtag aacgtaattt acctgccgga acttattcac tccgacaaga
     2581 acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg gccattttta
     2641 gcgcattgcc cgcttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt
     2701 attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa
     2761 acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca
     2821 atgccactcg cggtcagggt gcggtctggt ataaatggaa taactggctg acaacccgaa
     2881 cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata
     2941 ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac actaaatatt
     3001 acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc actctatact ggcccggtga
     3061 tcaccagcta ccgctatacc cattatgact ccagcgatgc aggtggtagt tatagcaata
     3121 tgatttccgt gcgtctgaat gacccgcgcg gcactggtta tacgcaacta tggctaagcc
     3181 gcggaacagg cgcttacacc tatgactgga cgccagaaac acgctacggc agcatgaaga
     3241 gcgtcagttt gcaacgtatt caaccgctga ctgagcaact taatctcggg ctgacggcag
     3301 gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc
     3361 tgacctggaa attctgattc cttctgccgc ccgctatccg gggcggcctt ccctgccgat
     3421 tagccccccc tttcctcttt gttttccgac cacattcacc ggataaattt tattctccag
     3481 tgttatatac tatagggggg tatgcattga catatagcat acccccctat agtatattgc
     3541 gtgcagatga tgaggtgcga aatgcccagt actccggaag agaagaaaaa ggtccttact
     3601 cgagttcgtc gtattcgagg gcagattgat gctctggaac ggtcgctgga gggtgatgcc
     3661 gaatgccgtg ccatactcca acagatcgct gccgttcggg gcgcggctaa cgggctgatg
     3721 gcagaagtgc ttgaaagcca tatccgggaa acgtttgacc gaaatgactg ctacagccgc
     3781 gaagtcagcc aatccgttga cgacactatt gaactggttc gagcctatct taaatagctg
     3841 aatctattac catattgagg aagagcgaga gatgaaatca cgtgctgccg ttgcatttgc
     3901 tcccggtaaa ccgctggaaa tcgttgaaat tgacgttgca ccaccgaaaa aaggtgaagt
     3961 gctgattaaa gtcacccata ccggcgtttg ccataccgac gcatttaccc tctccgggga
     4021 tgacccggaa ggtgtattcc cggtggttct cggtcacgaa ggggccggcg ttgtggttga
     4081 agtcggtgaa ggcgtaacca gcgtcaaacc tggcgaccat gtgatcccgc tttacaccgc
     4141 ggagtgcggc gagtgtgagt tctgtcgttc tggcaaaact aacctctgtg ttgcggttcg
     4201 cgaaacccag ggtaaaggct tgatgccaga cggcaccacc cgtttttctt acaacgggca
     4261 gccgctttat cactacatgg ggtgctctac attcagtgaa tacaccgtgg tcgcggaagt
     4321 gtctctggcc aaaattaatc cagaagcaaa ccatgaacac gtctgtctgc tgggctgtgg
     4381 cgtgaccacc ggtattggcg cggtgcacaa cacagctaaa gtccagccag gtgattctgt
     4441 tgccgtgttt ggtcttggcg cgattggtct ggcagtggtt cagggcgcgc gtcaggcgaa
     4501 agcgggacgg attatcgcta tcgataccaa cccgaagaaa ttcgatctgg cgcgtcgctt
     4561 cggtgctacc gactgcatta acccgaatga ctacgacaaa ccgattaaag atgtcctgct
     4621 ggatatcaac aaatggggta tcgaccatac ctttgaatgc atcggtaacg tcaacgtgat
     4681 gcgtgcggcg ctggaaagtg cgcaccgcgg ctggggtcag tcggtgatca tcggggtggc
     4741 aggttctggt caggaaatct ccacccgtcc attccagttg gtcactggtc gcgtatggaa
     4801 aggttccgcg tttggcggcg tgaaaggtcg ttcccagtta ccgggtatgg ttgaagatgc
     4861 gatgaaaggt gatatcgatc tggaaccgtt tgtcacgcat accatgagcc ttgatgaaat
     4921 taatgacgcc ttcgacctga tgcatgaagg caaatccatt cgaaccgtaa ttcgttactg
     4981 atttcccgca ggtatacccc gtccacttca gacggggttc ttaatactct ccccgggcag
     5041 ccgtccgggg gattaaccgt gagataatga ctgatggaac tcattgaaaa acatgccagc
     5101 tttggcggct ggcaaaatgt gtatcggcat tattcccaat cactgaaatg tgaaatgaat
     5161 gtcggcgtct atctcccacc aaaagccgcg aatgaaaaat tgccggtgct gtactggctt
     5221 tcgggcctga cctgcaacga gcagaatttc attactaaat cggggatgca gcgttacgcg
     5281 gctgagcaca acattattgt tgttgcgccg gacaccagtc cgcgaggcag tcatgtcgca
     5341 gatgccgacc gttacgatct cgggcaaggt gccgggtttt acctgaacgc aacgcaagcg
     5401 ccgtggaatg aacattacaa aatgtatgac tatatccgca acgagctgcc ggatttagtg
     5461 atgcaacatt ttccggcaac gaccagaaag tctatctctg gtcattctat gggcgggctg
     5521 ggcgcgctgg tgctggcgtt acgtaaccca gatgaatatg tcagcgtctc ggcgttttcg
     5581 cccattgtct ccccatcgca agtaccgtgg ggacagcaag cctttgccgc atatcttggt
     5641 gaaaataaag atgcctggtt ggattacgat ccggtgagtc ttatttcaca aggtcaacgc
     5701 gttgcggaaa tcatggttga tcaggggttg agtgatgatt tttacgcgga acagctgcgg
     5761 actccaaatc ttgaaaagat ctgccaggag atgaatatca agacgttaat ccgttatcac
     5821 gagggttacg accacagcta ttattttgtc tccagtttta ttggcgagca tattgcctac
     5881 cacgccaata aactgaatat gcgttgataa tagtgcacga ctgccggatg cggcgtgaac
     5941 gccttatccg cctacatttc gcccgcaggc cgtaggcatg ataagacgcg catagcgtcg
     6001 catcaggcat ctgcgcacga cttatctatg tttaccacat caaatcatca ggcactttga
     6061 agtcggcata cggatcgtct tcatcctgct cttcggcact gagcgcgctg tgcaacacta
     6121 tactgctggc atcacgctga gctattttat cggcaacgct ggcgggaata atggcgtatt
     6181 caccttcgct attgttatcg accaacaagc gagcaatcgc cagacgacca ttaatcaact
     6241 gcgcctgtgt gagcttatcg acaaaaatct tcttaatcag attgccctca gtgaagttaa
     6301 aaccaatatc gccattggca atggtgattc ggttcatttc aatgagctgc ttcacctgag
     6361 ctttatattc tttcgccaac gcagcttgtt tttgttgctc gctaagctgt ttatcacgct
     6421 caagctgtgc ctttttattt tcttctaccg ccgcccgagc ttcacgcgcc tgaacgcgcg
     6481 attttttcgc cgttctctcc accttcgccg cttttttgct ggtgactaat cctgctttga
     6541 gcaactgctc ttgtaaggta agttttgcca tctttgtttc caaacccgct gaataatttt
     6601 tagcgagtat acctgtaaac gccgtgggtg tattgggggc aagtcccttt cgcccctgct
     6661 aaataaggcg cgtcaggcat cggcacacgg ctgcattctt gatctccgac tcatcaaaat
     6721 aaacaccgcc aacccagcaa caagaatacc cggtgcagaa gcggccatta cgccgaccgt
     6781 gccagtgcct aatgccagca ttttcccggc cagtaacgga ccgctcatag cccccagacg
     6841 ccctaccgcc acggctgttc ccacacctgt tgcgcggatc tgcgaactgt aaaacaacgg
     6901 tgccagggca tacaaaacgc tttgcccacc tgtcgcaaac aaccccgcga caaatcccgc
     6961 cagcaacata ccgttaaatg acgacaccgt tccaagcgcc agcagcgaag ctaacatgcc
     7021 gctataaatc agtagcgaca tggttactgg acgcagctta tccatcaatg cgcccaacat
     7081 taacgtcccg cttgccgccc ccatttgcag ggcaaacatc acccctgccg cctgcgatgg
     7141 ctggaatcct tgctccacca aaagtagcgg tagccagttg atcaacatat aaaccaccag
     7201 cagagtgaag aaataacaca accacagcag cagcgtcgcg gttgccgttt ctggcgcaaa
     7261 taaggcacgc agtggtggcg cagcctgttt ttcgccagcg aaaaccgctg actccggcag
     7321 ccagcgcatt aatagcggca ccagaatcaa cggcaccaca ccacctaccc aaaacaccgt
     7381 ttgccatgct aagtttgccc ccgcgaaacc cagtgtcgcc gccagcgccg cgccaatggg
     7441 aacaccgcaa tacatcaggc tcactgccgt cccacgaaaa cgtggacccg cggcttcaga
     7501 cgtcagagcg ataagattcg gcaacgccgc ccccagcccg acaccggtca tcagccgcgc
     7561 aaagaccagt gaggggaaat cccaggcaat cgccgttgcc agtgagaaca aaccaaacag
     7621 cgcaactgag ccaatcaaaa tacgcttacg accataacgg tctgccagca tcccgccaac
     7681 caacgcgccg ggtagcaaac cgagtattcc ggcgctaaat atccagccca tttgcatttt
     7741 atcgagtgcg aaagcctggg cgatgccacc cgccgcaatg ccagccgcct gaagatccag
     7801 cccttccatc agagcgacca aaaaacaaag cccgatggtc agcatcaggc gggatgaaga
     7861 tgatgaaggg gtacgagtcg acataagatg aacctcattg cgagtgcagg tgaatcgcga
     7921 ctgatacatt gccagccgcg taatcagaac tatttaatga ctgcctacgg gcgaaatgta
     7981 ggccggataa ggcgttcacg ccgcatccgg ctgtcgtgct gggatgggca tacaaaatgc
     8041 acgttatcgg cagtcatcat tatttgttgt tgcgcagatc cagcgccacg tcgacgatca
     8101 tatcctcctg gccgccaacc atccggcgtt tgcccagctc aacgagaata tccaccgcac
     8161 ttaagccata acgcgccgcc gccgtttcac agtgacgcag gaagctcgag taaacaccag
     8221 cgtatcccag cgccagcgtt tcgcgatcga ctcgtaccgg tcgatcctgc aacggacgca
     8281 ccaggtcgtc ggcggcatcc attaacgcat agagatcggt cccatgctgc cagcccagtt
     8341 tatccgcggc ggcaataaac acttccagcg gtgcgttacc tgcgcccgcg cccattcccg
     8401 cgaggctggc gtcgattcgg tcgcagccct cttccaccgc cgcgatagag ttcgccacgc
     8461 caagactcag gttatggtga gcgtgcatgc cagtttgcgt ttctggtttc agctctgctt
     8521 tcagggcgcg gaaacggtca cggatatcgc tcatgttcat cgcaccgcca gaatccacca
     8581 cataaataca ggtcgcaccg tagccttcca tcagctttgc ctgcttggcg agattctccg
     8641 gcgtggtcat atggctcatc atcagaaaac caacggtgtc cattccgagc tcgcgggcat
     8701 actgaatatg ctgggcggaa acatcagctt cggtacagtg cgttgccaca cgaaccaccc
     8761 gcgcgccagc ctgccaggca tttttcagat cgtgaatagt gccgattcct ggcagcaaca
     8821 acgtcgcgat tttggcgtgc ttcaccacat ccgccgctgc ttcaatccat tcaaggtcgc
     8881 tatgtgcgcc gaaaccatag ttaaagctgg aaccctgcaa accgtcgcca tgggccactt
     8941 caatcgaatc cacgcgggca tcgtccagtg ctttggcaat ctggcgaacg ttttccagcg
     9001 aatactgatg acgaatggcg tgcataccgt cacgcaacgt gacgtccgag atataaagtt
     9061 ttttaccgtt catgccgctt ctcctgcctt gcgcgccagt gactgggcca ttttttccgc
     9121 tgtcgccagc gcactggaag tcataatgtc gaggttgccc gcataggcag gcagataatg
     9181 cgctgcgcct tcgacttcca gccagaccgc tgttttcagt ccggagaatt gccccacgcc
     9241 cggtaatttg accggtttat cctgcgggat aacttcaaac tgcacgcgct gtttcaggcg
     9301 ataacccggt acgtaagcct gcaccgcctc agccatttca ttgattgagg cttcgatatc
     9361 atcttgtgaa gcttcgtcgc tcaatacata caccgtgtca cgcatcatca acggtggctc
     9421 tgctgggtta agcacaataa tcgccttccc ttttgccgcg ccgcccacca cttcaatggc
     9481 tcgggaagtg gtttccgtaa attcatcgat attggcacgc gtgccaggtc cggcagattt
     9541 actggcgata gaagcgataa tttcggcgta atgaacacgc gccacgcgtg aaactgccgc
     9601 caccattgga atggtggcct ggccgccgca ggtgaccatg ttgacgttca gttgatcgac
     9661 gttcgcctcg aggttaacca ccggcacgca gtaagggccg atggcagcag gcgtcaggtc
     9721 aattaagcga atatccggtt tcgcttcgcg taaagcggca tcgtttttca catgcgcgcc
     9781 cgcgctggtc gcatcaaata caatgtcgat atcagcaaat tcaggcatgt tcatcagccc
     9841 gatcacccct tcatgggtgg tggcgacgcc catacgtctg gcacgcgcca gaccgtcgga
     9901 cttaggatca atgccgacca tcaccgccat ctccagatgc tgaccgtgac gcaaaatttt
     9961 aatcatcaga tcggtgccaa tgttgccaga accgataatg gcgactttac gcttactcat
    10021 gacagacttc cttttggggc tgcgcttgaa aatgtcgcag caactgaacc tatgccttca
    10081 atatgggctt caaaacgatc gcccgcattc accgccacca tcgggcctaa tgccccggta
    10141 agaatgatat ctccggcgcg cagcggttca cccagactgg ccattttgcg tgccagccag
    10201 acggcggcat taagcggatg tcccaggcat tcgctgccgc gcccgctgga aacctcttcg
    10261 ttattacgcg tcatcttcat ggcgcagttt ttcaggtcta accccgccgg acgttgcgcc
    10321 ggaccgccga tgacatacac cccgcaggaa gcgttatctg ccacggtatc gacaaactga
    10381 atcgaccagt cgcgaatgcg gctccccacc acttccagcg ccggaagtac ccattcaatg
    10441 gcgttataca gttcgtcgaa ggtgatatca gttgcgggta aatcgcggtt caacaccagt
    10501 gcgatctccg cttcaatgcg gggttgcaga acacgggaaa aaggaatgat ttcgttatcg
    10561 ccataacaca tgtcggcaaa taacgtccca aaatccggtt gatcaacgcc cagttgttgt
    10621 tgcactttcg gatgcgtcag gcccacttta cgccctacca cgcgacgccc ctgcacaacg
    10681 tcatattgca catttatgtg ctgaatggcg taggccgctt cagcgttatc gataccaatc
    10741 agatcgcgca gcggcgcaat cgcttcgccc tgctctgcgg cgcggcgtaa atccgccgcc
    10801 agttgctcaa gagtatgctt cgtcatgacc attccttaag ggcgtgcgag gaaattcagc
    10861 accaattgat tgaaagcgtc ggcatgttcc cactgcgccc agtgaccaca gtcgcggaag
    10921 atatgcagtt cagaaccggc aatgccggac agcagacgca gacccgcatc catcggcaca
    10981 aagcggtcgt tgcgccccca gacaatcagg gtttgcgctt tgatttccgc cagacgtggg
    11041 ccaaaatccg ggaactgttt cggattagct tccaggctct taacgaagtt ttccaggtga
    11101 tcgcggcgcg acagcatatt attcaggcgc gcttcaaaca gggcgtcggt caaatcgctg
    11161 gtatcaaaaa cgaagatatc catcatcagc ttcaggtttt cgatagtcgg ctgacgataa
    11221 agctgattca gtcgcttaat accttcggtt ggcatcggcg taaacaaact catgccgccc
    11281 gtaccgccgc ccatcagcac cagtttgccg acgcgctccg gccatttaag ggtgaacgcc
    11341 acagaactat ggccccccat cgagttgccc agcaggtgga ttttggcgat atccagttga
    11401 tccaccacgc ttttcaggat tcgtgcatta agatccgatc gtgaaccact attaacgatc
    11461 gaatcgcttt tgccccaacc cggacaatcc agcaggatta cccgatagcc cgcctctacc
    11521 agcggatcga tattgcggct gaagttcgcc cagccagtag cacccgggcc ggaaccatgc
    11581 agcaggacaa cggtttcgtc gccttgtccg cagtcattaa aatggatgcg cagcgtttta
    11641 cccgcttctt ctacattcag aaaacggctg gtggcggctt cggtttgtgg ctgataactc
    11701 atcatcttct cctgcatatt cagttctctg ttctggcgct taacgagcca aatccggcaa
    11761 tccactccgg gattgggcgg taataacgcc cttcgctacg ccagttgcca aatgtagaaa
    11821 tagcggcaaa agcggcgacc caggttttga tttcatgtgt cgactttccg gcaatggcgg
    11881 aaagctcttc gttactgacg gcatccagtt cctgtatgcg tccctgctcc agcaaagtca
    11941 tgaactggtt atcccaaatc gggttgagcg gatgcagagt tctctgatcc tcaacaaact
    12001 tctcagcggc gctaatcacc cgttgctgac gcaattcgcg ctcactggcg ggtaaatctt
    12061 tcccgctccc caacagacgg tcgcgcatat gggcatcggc tttcgccagt tcgggcaccg
    12121 gcggctgatg ggaaagccca ccggaaccca ggaacagcac gcgtttattg agagtgctgg
    12181 tgaaacgtcc aatggcttca cccaacatgc gggtacgctg gaaaccgggc agcggcgtgg
    12241 cgacaccgtt gatgaacaca ggcagaactg gcaccttatc cagcccaccg agcaggaact
    12301 ccagcggctg ggcgaacccg tggtccacct gcatacagta agaaacggca agatcgatcc
    12361 cgctcttcat gacagcatgc gcacaggcct ccgccagctc cacaggcacg ggcagctctc
    12421 ctgccgcact gccgaaatca ccaattgccg tcgctccaac gcctaaacag aacggtggca
    12481 tcacgtcata gaaaaagccg ttgtagtgat ctggcgcaaa cagcaccacc agttcagggg
    12541 agaaagccgc aatacgctcg cgggcgctgg caatcacgcc attgacctca tcgagtacct
    12601 cttgcgccgg gtcgacatac cccaccaacg gcgagtggga aagacagtga agataagcgt
    12661 gcatatcagg ctaccttttc gacagaaacg tcggcatcag ggcgggtcag cgtcatcacc
    12721 gacgccagtt tattcagcgt attgcccagg gtttgcggaa tggcggtggc ggcaacaaag
    12781 cgatccgggc gcatcaccac cagcgaagca ttgtgttgcg cgaaccagct acgcagacga
    12841 ccttgcgtat cgcccacgcg tagcacgccg tcgtggttat cctgtgcggt atgaatttgc
    12901 acttccggca ccacctgaat gaagcgtgtg cccaacgcgc gccactgctg gatttgctcg
    12961 tcgctcatcc cccacagtgg attgcatccc cagccaatta ccgcgaagtt cgcgccaatc
    13021 gcgttatcga gcagcgtcac gtcgccgttt tccagcgtga ctttcggctg aataaacatc
    13081 ttgccgaccg gagagtgctt cgcttcgccc tcacgtacca gcgcaccgcc gtaatattgc
    13141 ggcatcggct tgaagcgcat ttcgaggaag tagcgtttta ctggcggcag ataattcagt
    13201 agccaggaaa cgccgtcacg taacgtaccc tgccagcgtt tcggcggagc cagcacgttg
    13261 cccgtcgtca cggacaggtc aatcatcgct ttggcgtgat cgcgacgttc ttgttgatag
    13321 gtatcgagca gcgcatcgcg ggctttcccc tggataacca acgccagttt ccatgcgagg
    13381 ttaaaggcgt cgcgcatacc actgttatag ccctgcccct gccataccgg catgatgtgc
    13441 gcggcatcgc ccgccagcag tacgcgatca atacggaaac gttgcgccag tcgcgcgttg
    13501 tgggtgtaga cacgctggcg aatcaattca acattgtccg gattaggcag cactttgctt
    13561 aacagcttgc gcatattttg cggctcacgc agctgttctt cggtttctcc cggcatcacc
    13621 ataaattcaa agcgacgtac cgcatgaggc agcgcggcag aaacatacgg gcgcaccgga
    13681 tcgcaacata aatagatatg cggcgtactt aacggatcgt tggcgatatc caccacaatc
    13741 cactgatttg gcgcagtttt accttcaaac ggcacattca gggtgcgacg aacaaaactt
    13801 gccccaccat cacaggccac cagccactgg gctttgacta tttcccgctg cccttctgcc
    13861 gttttcaggt gcaaggtcac ttcgtcattt tgctggctga aggcctccag ctcgcgggaa
    13921 aacaagcagc gcacattagg aaaacgcgac accccttcca gcatcaccgc atcgacctgc
    13981 ggctgaataa aggcgttacg gcgcggccag ccaaattcat cggtcattgg ctgaatatca
    14041 gcaaaacagc ggcctttcgg ggtgagaaaa cgcatcgcgt gccacggcgt agtgtgcggc
    14101 agaacattct cgaccaggcc aaccgactgc atggtgcgca gcgcctcgtc atcaatacca
    14161 atcgcacgcg ggtagtcgat caacttatcg agtttctcca ccaccagcac gtcaatgccc
    14221 atctgaccga gatagttcgc catcatcagc ccaaccggac cggcaccagc gatcgccacc
    14281 tgaacgctat ggttaacagc aggctggatg tcagggtgtt gtattgccat ttcagtacct
    14341 cacgactcgg acaaaatgcc gttgcgcgca cagtacggcg caacttattt tgttaaaaac
    14401 atgtaaatga ttttttattg tgcgctcagt ataggaaggg tgttttcggc tacaatcaaa
    14461 acatgcccga atgtgcacca ggtgcacaac gttgttttaa ctatagaaat gtcaattaat
    14521 atgcagaaca atgagcagac ggaatacaaa accgtgcgcg gcttaacccg cggtctaatg
    14581 ttattaaata tgttaaataa acttgatggc ggtgccagcg tcgggctgct ggcggaactc
    14641 agcggcctgc atcgcaccac tgtgcggcga ctgctggaga cgctgcagga agagggatat
    14701 gtccgccgta gcccctccga tgacagtttt cgtctgacca tcaaagtgcg gcaattaagc
    14761 gaaggatttc gtgacgaaca gtggatttct gcactggcgg ccccgctgct gggcgatctg
    14821 ttgcgcgaag tggtatggcc aacagatgtg tccaccctgg atgttgatgc aatggtggta
    14881 cgcgaaacca cccaccgttt cagccgctta tcctttcacc gggcaatggt cgggcgacgt
    14941 ttaccgctcc tgaaaaccgc ctcgggcctg acctggctgg ccttttgccc ggaacaagaa
    15001 cgcaaggaat taatcgaaat gttagccgcc cgccccggtg atgactatca actggcacga
    15061 gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca aagagggtta cggacagaac
    15121 taccgcggct gggatcagga ggagaagatt gcctctatcg ccgtaccgct gcgcagtgaa
    15181 caacgggtga ttggctgtct gaatctggtg tatatggcga gcgcaatgac cattgaacag
    15241 gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa aacagatcga agaaggggtt
    15301 gaatcgcagg ctattctggt ggccggacgg cgaagcggcg tgcatttacg ttgacaccat
    15361 cgaatggcgc aaaacctttc gtggtatggc atgatagcgc ccggaagaga gtcaattcag
    15421 ggtggtgaat gtgaaaccag taacgctata cgatgtcgca gagtatgccg gtgtctctta
    15481 tcagaccgtt tctcgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgagaaaa
    15541 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
    15601 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
    15661 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
    15721 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
    15781 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
    15841 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
    15901 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
    15961 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
    16021 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
    16081 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
    16141 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
    16201 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
    16261 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
    16321 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
    16381 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
    16441 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
    16501 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    16561 ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat tgtgagcgga taacaatttc
    16621 acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
    16681 gactgggaaa accctggcgt tacacaactt aatcgccttg cggcacatcc ccctttcgcc
    16741 agctggcgta atagcgaaga agcccgcacc gatcgccctt cccaacagtt gcgcagcctg
    16801 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
    16861 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
    16921 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt
    16981 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
    17041 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc
    17101 aacggtcgct gggtcggtta cggtcaggac agtcgtttgc cgtctgaatt tgacctgagc
    17161 gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc
    17221 agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg
    17281 ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat
    17341 ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta
    17401 cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc
    17461 ggcggtgaaa ttatcgatga gcgtggtagt tatgccgatc gcgtcacact acgtctgaac
    17521 gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa
    17581 ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag
    17641 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt
    17701 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag
    17761 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac
    17821 catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat
    17881 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg
    17941 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc
    18001 atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg
    18061 atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg
    18121 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct
    18181 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc
    18241 ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag
    18301 gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg
    18361 ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat
    18421 acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat
    18481 ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa
    18541 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg
    18601 atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca
    18661 caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc
    18721 tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc
    18781 agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc ctccgcgtcc
    18841 catatcatcc cgcaactaac caccagcgaa acggattttt gcatcgagct gggtaataag
    18901 cgttggcaat ttaaccgtca gtcaggcctt ctttcacaga tgtggattgg cgatgaaaaa
    18961 caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc
    19021 gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc
    19081 cattaccagg ccgaagcagc gttgttgcag tgctcggcag atacacttgc cgacgcggtg
    19141 ctgattacga ccgctcacgc gtggcagcat cagggaaaaa ccttatttat cagccggaaa
    19201 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtagcgagc
    19261 gatacaccgc atccggcacg gattggcctg acctgccagc tggcgcaggt cgcagagcgg
    19321 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgcggcctgt
    19381 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa
    19441 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
    19501 ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat
    19561 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
    19621 ggcgacgact cctggagccc gtcagtgtcg gcggaattcc agctgagcgc cggtcgctac
    19681 cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
    19741 tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
    19801 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
    19861 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
    19921 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cgggctgcgc
    19981 aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgccgtt ctttattttt
    20041 atcttcgggc cactgttaca atacaacatt ttagtaggat cgattgttgg tggtatttat
    20101 ctaggctttt gttttaacgc cggtgcgcca gcagtagagg catttattga gaaagtcagc
    20161 cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg gctgtgttgg ctgggcgctg
    20221 tgtgcctcga ttgtcggcat catgttcacc gtcaataatc agtttgtttt ctggctgggc
    20281 tctggctgtg cactcatcct cgccgtttta ctctttttcg ccaaaacgga tgcgccctct
    20341 tctgccacgg ttgccaatgc ggtaggtgcc aaccattcgg catttagcct taagctggcg
    20401 ctggaactgt tcagacagcc aaaactgtgg tttttgtcac tgtatgttat tggcgtttcc
    20461 tgcacctacg atgtttttga ccaacagttt gctaatttct ttacttcgtt ctttgctacc
    20521 ggtgaacagg gtacgcgggt atttggctac gtaacgacaa tgggcgaatt acttaacgcc
    20581 tcgattatgt tctttgcgcc actgatcatt aatcgcatcg gtgggaaaaa tgccctgctg
    20641 ctggctggca ctattatgtc tgtacgtatt attggctcat cgttcgccac ctcagcgctg
    20701 gaagtagtta ttctgaaaac gctgcatatg tttgaagtac cgttcctgct ggtgggctgc
    20761 tttaaatata ttaccagcca gtttgaagtg cgtttttcag cgacgattta tctggtctgt
    20821 ttctgcttct ttaagcaact ggcgatgatt tttatgtctg tactggcggg caatatgtat
    20881 gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc tggtggcgct gggcttcacc
    20941 ttaatttccg tgttcacgct tagcggcccc ggcccgcttt ctctactgcg tcgtcaggtg
    21001 aatgaagtcg cttaagcaat caatgtcgga tgcggcgcga gcgccttatc cgaccaacat
    21061 atcaggacgg aatgatcgca atgaacatgt cgatgacaga aagaataaaa gcaggcaagc
    21121 tatttaccga tatgtgcgaa ggcttacctg aaaaaagact tcgtgggaaa acgttaatgt
    21181 atgagtttaa tcactcgcat ccatcagaag ttgaaaaaag ggaaagcctg cttaaagaaa
    21241 tgtttgccac ggtaggggaa aacgcctggg tagaaccacc tgtctatttc tcttacggtt
    21301 ccaacatcca tataggccgc aatttttatg caaatttcaa tttaaccatt gtcgatgact
    21361 acacggtaac aatcggtgat aacgtactga ttgcacccaa cgttactctt tccgttacgg
    21421 gacaccctgt acaccatgaa ttgagaaaaa acggcgagat gtactctttt ccgataacga
    21481 ttggcaataa cgtctggatc ggaagtcatg tggttattaa tccaggcgtc accatcgggg
    21541 ataattctgt tattggcgcg ggtagtatcg tcacaaaaga cattccacca aacgtcgtgg
    21601 cggctggcgt tccttgtcgg gttattcgcg aaataaacga ccgggataag caatattatt
    21661 tcaaagatta taaagttgaa tcgtcagttt aaattataaa aattgcctga tacgctgcgc
    21721 ttatcaggcc tacaagttca gcgatctaca ttagccgtat ccggcatgaa caaagcgcag
    21781 gaacaagcgt cgcatcatgc ctctttgacc cacagctgcg gaaaacgtgc tggtgcaaaa
    21841 cgcagggtta taatcatcag cccaacgacg cacagcgcat gaaatgccca gtccatcagg
    21901 taattgccgc tgatactacg cagcacgcca gaaaaccacg gggcaagccc ggcgatgata
    21961 aaaccgattc cctgcataaa cgccaccagc ttgccagcaa tagccggttg cacagagtga
    22021 tcgagcgcca gcagcaaaca gagcggaaac gcgccgccca gacctaaccc acacaccatc
    22081 gcccacaaga ccggcaattg cagcggcaac cagataaagc cgcagaaccc caccagttgt
    22141 aacaccagcg ccagcattaa cagtttgcgc cgatcctgat ggcgagccat agcaggcatc
    22201 agcaaagctc ctgcggcttg cccaagcgtc atcaatgcca gcaaggaacc gctgtactgc
    22261 gcgctggcac caatctcaat atagaaggcg ggtaaccagg caatcaggct ggcgtaacca
    22321 ccgttaatca gaccaaagta aacacccagc gtccacgcgc gaggagtgaa aaccacgcga
    22381 accggagtgg ttgttgtctt gtgggaagag gcgacctcgc gggtgctttg ccaccaccag
    22441 gcaaagagcg caacaacggc aggcagcgcc caccaggcga gtgtttgata ccaggtttcg
    22501 ctatgttgaa ctaaccaggg tgtcagggcg gcaccaagcc caccgccgcc catcagagcc
    22561 gcggaccata gccccatcac cagtggcgtg cgctgctgaa accgccgttt aatcaccgaa
    22621 ggcatcaccg cctggatgat gccgatcccc accccaccga gcagtgcgct gctaagcagc
    22681 agcgcacttt gggggtaaag ctcacgcatc aatgcaccga cggcaatcag caacaaactg
    22741 atggcgacgc tgcgacgttc gctgacatgc tgatgaagcc agcttccggc cagcgccagc
    22801 ccgcccatgg taaccaccgg cagagcggtc aacagggcag ccacgctaaa gctcattccg
    22861 ctcgcctggc gcaattgcgg tagcagtggc ccgacggagg tgagcagtgg tcgcatatta
    22921 agaccaatca gcaccagaac cagcagtatc gtgaacgtct tttgatggtt gcgcatggct
    22981 gtcagaacgg tttggtcggc agatatttac catctaaggt gatgaccgca cgttcgccac
    23041 cttccgggtc cgccactttc ttaacgtcga gtttgaagtt aatcgcgctg ataatgccat
    23101 cgccaaactt ctcatgaacc aacgctttca gggttgtgcc gtacacctgc aacatttcat
    23161 agaaacgata catcgttggg tcggttggaa tacggtcatc aatgcagcca cgcagtggaa
    23221 tcatctgcaa cagtagaatg gcgtcttcgt cgagatccag cttcgctccg accaggcggg
    23281 cggcgtcggc aggaagcgcc tgctgcccca gcaaagccgc ggttacgaag gcttctgcca
    23341 gaccggtacc gtcggcaatc tcggcaaatg agagatcttt tttagctttg ctgagcaaaa
    23401 tggcatcggc aagatcaaga cgaatattgc ggttaatttg cgactgaatc atggtgcatc
    23461 tcctgatggt ttaaaaataa ggttacgctg cggtcggttg gcgtagcggt atggcacaaa
    23521 cgcgcggatt agctgccagt ggcacaaact ggcgggttgc gccgtcaaac gctgcgatgc
    23581 tgccgctttc aatgtcgtag acccagccgt gcagggcgat ccgcccctct tcgagcgcca
    23641 ggcgcaccga tggatgagtt tgcaaattag ccaactgagc aatgacgttt tcacgtacca
    23701 tcgccgcagc ttttgacggt aaatcggaat gcgggcgcgc ctcattaacg acgcgggctg
    23761 aatcggcata acgcagccag tgggagacgg caggcatatg gtccatgcac tgacagctgg
    23821 caatggcggt catcgcgcca cagttggaat gaccgcaaat cacaatgtca gatacccgaa
    23881 gcgcagcgac ggcatactcc accgaagcag aaacgccacc gggttccggc ccgtaggaag
    23941 ggacgatatt gcccgcgttg cgaataacga acagatcgcc aggctcacgt tgcgtcacca
    24001 gctcagggac cagacggctg tcggagcagg agataaaaag tgtgcgcggg ctttgctgtg
    24061 tcgccagctg tttaaacaag gcttcccgct tcggaaatgc ctcgcgctgg aatttaagga
    24121 atccatcaat aatctctttc accgttaacc tctgtctgtc tctggaatga gaggcagact
    24181 actcgccgat tgtcataagg taaaagtctc atttatgatg agttctattg gatttactta
    24241 taggttggcg aatgctctct cgacatatca attattttct tgccgtggct gaacatggca
    24301 gcttcacccg tgccgccagt gcgttgcacg tctctcagcc tgcgctttcc cagcagattc
    24361 gccagttaga ggagagttta ggcgtgccgc tgtttgaccg tagcgggcga acgattcgtc
    24421 tcaccgatgc aggagaagtc tggcgacagt acgccagccg ggcgttacag gaactgggag
    24481 cgggtaaacg ggcgattcat gatgttgccg atctgacgcg aggatcgctg cgtatcgccg
    24541 tcacccccac ctttacaagc tactttatcg gccctttaat ggcggatttc tatgcgcgct
    24601 accccggcat cacgctccag ctacaggaaa tgtcgcagga gaaaatcgaa gatctgcttt
    24661 gtcgcgacga gctggacgtt gggattgcct tcgcgcctgt gcattcgccg gagctggagg
    24721 caattccttt actgacagaa agtttagcgt tagtcgtggc gcaacatcat ccgctggctg
    24781 cctgtgaaca ggtggcgttg agtcgcttgc atgatgaaaa actggtcctg ctcagcgcgg
    24841 aatttgccac cagagagcaa attgaccact actgcgagaa agcggggcta catccacagg
    24901 tggtcattga ggcgaactca attagcgcgg ttctggagct gattcgccgc acttcccttt
    24961 ccacattgtt accagcagcg attgccacac aacatgacgg gcttaaagcg atttctcttg
    25021 ccccgccact actggagaga acggcggttt tgttgcggcg gaaaaatagc tggcagacag
    25081 ccgccgcgaa ggcatttttg cacatggcgt tggaggaatg tgcggatgtt ggcgagaatg
    25141 aatcacggta gccactgaca caggtcagtg gctcttcaac acagaggaaa ttaccgtttg
    25201 taatcgatgg cttctggctg ctccagatat acggtggttt gtgccggttg tgtactggca
    25261 atcaccttgc cgccacgtac cgaataacgt accggaacct gacggcgcag cgcatcaaat
    25321 ccattttcag ccggcaggat aatcaggttt gcgctgtttc cggcggcaat gccgtaatcc
    25381 tgcaaattca atgttctggc actgtggtgg gtgattaaat tcaggccatc gttaatctgc
    25441 ccgtagccca tcaactggca aacatgcagc cccatatgca gcacttgcag catattcgcc
    25501 gttcccagcg gataccacgg atcgaagaca tcatcgtgac caaagcagac gttaatgccg
    25561 gactccagca tctctttaac gcgcgtgatg ccgcgacgtt ttggatacgt atcgaaacgt
    25621 ccttgcagat gaatattgac cagcgggttg gcgacaaagt taataccgga cattttcagc
    25681 aagcggaaca ggcgtgaggt atacgccccg ttataggagt gcattgccgt ggtgtggctg
    25741 gcggtgactc gcgcgcccat gccttcacgg tgcgccaggg cagcaacggt ttcgacaaag
    25801 cgcgactgct cgtcatcgat ctcatcacag tgaacgtcga tgagacggtc gtatttttgc
    25861 gccagggcga aggttttatg cagcgactcc acgccgtatt cacgggtaaa ttcaaaatgc
    25921 ggaatcgccc ccactacatc tgcccctaag cgtaacgcct cttccagcaa cgcttcaccg
    25981 ttgggatacg acaaaatccc ttcctgaggg aaggcgacaa tttgcagatc aatccacggc
    26041 gcgacttcct gcttcacttc cagcattgct ttcagcgcag ttagcgttgc atccgaaaca
    26101 tcgacatggg tacgcacatg ctgaatgccg ttggcaatct gccatttcag cgtttgccag
    26161 gcgcgttgtt tcacatcgtc atgggttaat aacgctttgc gctcggccca gcgttcaatg
    26221 ccttcaaaca gcgtgccgga ctgattccag ttcggttgtc cggcggtttg cgtggtgtcc
    26281 aggtgaatat gcggctccac aaacggcggt ataactaaac cttgttcggc atccaggctg
    26341 ttttcagtta tgggcatcac gccggattgc gcatcaatgg cgctgatttt tccgtcctgc
    26401 agatgaatct gccacagccc ctctttgcct ggtaaccggg cgttaataat tgtttgtaaa
    26461 gcgttattcg acactgttaa cctccacatg cgtcattgct gctgtcgttt tacgattcaa
    26521 aatcgggtta aggatcagat agctcagcgc gccacctaat accgcgttga ccggaacaat
    26581 tcccggtaac cagtggcctg cggcaatccc cagcgcgacc gccagaatcg ccacccaatt
    26641 gacactcatc atacgcgtgg tcgcaaagtg ctcatagcgg cgacggttca tcagatagtc
    26701 ggcgatgatc acgcccccta ccggaggaat agctgccgaa aggaaggtca accagccgac
    26761 aaaattgtta tacagccata atgcgcagac cgtaccgata ataccgttga ttaccgaaag
    26821 ggttttgctc gacataccgg taatgttggc gaaacctaaa cccgacgcat agagtgcgtt
    26881 atcgttggtg gtccagatat tcagccccag caccacaatc gcgggcagca gcagtccctg
    26941 agcaatcatc acatcagaga tatctgccat ccccagtgcc gcagcgcccg ctgcaccgaa
    27001 aataaacatc aacgagttgc cgaggaaaaa ggccaccatc gccaccagca ccgccagttt
    27061 ggcattgcga ccaaaccgga caaagtcagc ggtgagcgta cccgcactga taaatgaccc
    27121 cacaaccagc gccagcgcga cattgaaatc taacggttgt gcgggaacga ccgcttttaa
    27181 ggcgtccagg ccgcccatgc cgttaacggc cagccacacg gaataaccgc caaggcaggc
    27241 aatagccgga accgcaatca ccgaaagaac tgtcagcgcc gaaatgccaa agaagacggt
    27301 gacggtcatc agtaaaccgg aaacggcaat cagcaaatta atatccagcc cggttgcctt
    27361 gcccaccgga atagcaaaca tcgccacgcc aacgccaaac cagccaacct gagttccgcc
    27421 cagtagcagt gaaggcagcc atgagccttt aacaccaaac gagaagcgag caagaagatg
    27481 agtggtcagg ccggtttttg cgccaatgta accgagaaat gaagtgtaaa taccgaggag
    27541 aagattaccg atgagaactg cgaggaagaa atcatgatag ctaagaccgg ttccgagagt
    27601 gccgccggtc cacatactgg cggaaaagaa ggttaatccc agcatgacga acgtcaatgc
    27661 caatacccct ttccgcgccg actgcgggac tggcccctgg ctaaagttgt tatcttgcga
    27721 cacgaaattc cccctttttg ctgtttgaaa cccaaaaaat ccgccgcatt ctattcatct
    27781 gaagataaaa agcaatcgtt ttcgtgggga aatatatttt ttatatgaat gagtgcaaaa
    27841 aggtggggga aaaattatcg gtgtaaaaac agtgggtagg cataataaga cgcggtaagc
    27901 gtcgcatcag gcatcggcgc ggggtgtcgg atgcgacgcg agcgccttat ccgacctacg
    27961 gttcaggcct gtaggcataa taagacgcgg taagcgtcgc atcaggcatc ggcgcatggt
    28021 gtcggatgcg ccacgcgacg catccgacaa taccttactc ttccatcgcc tggcggatct
    28081 gatccaatga cgcaggatca tcaatggtcg tcagatcccc aggatcgcgt ccttcgcaaa
    28141 ttgcctggat cgtgcggcgc agcatttttc cggatcgcgt tttcggcaat tgcgagacaa
    28201 accagacgtg cgccgggcga ccaaagttgc caatctggct gtccaccagc gccataatcg
    28261 ccttctcttg cgaatgcgcc acatcacgat cttccagact atcgctctct ttcggaatga
    28321 caaacgccac cgccacctgc cctttcagcg catctttcac cccaaccacc gccacttcgg
    28381 caacgcccgg atggctggag atactctctt caatctcgcg cgtgcccaga cgatgcccgg
    28441 caacgttaat tacatcgtca gtgcgcccga gaataaagtg ataaccgtca gcgtcacgga
    28501 tgccccagtc aaaggtggcg tacaccgggc gggaaaacag cgaccagtaa gtcttcacaa
    28561 agcggtcgtc gtcgccccag atggtctgaa tacagcccgg cggcagcggc ccttccacca
    28621 ccagcatccc tttctcgttg acgccacacg gttcgccggt gacttcatta agcaactgca
    28681 cgttatagcc atacatcggc acgccggggc ttcccagacg cgtcggcctg tcgtccagac
    28741 cgcgagcaat cgccataatc ggccagccgg attcggtctg ccagtagttg tcgatgaccg
    28801 gcacatccag cgtattgctc acccaactgg cggtcggctc gtccagcggt tctccagcca
    28861 gatagagcac ttccagcgac gagagatcgt gtttgcgaat ttcagcggta gggaattttt
    28921 tcagcacgcg aatggcggtc ggcgctgaga acatccggct aacctgatat ttctcgacga
    28981 ttttccacca cacgccgcag tccggccagg tcggcaatcc ttcgtaaacg atagtcgcca
    29041 tccccgccag cagcggcgcg taaacgatat acgaatgccc caccacccag ccgatatccg
    29101 atgcgcaaaa gaacacgctg cccgctttgc cgccaaaaat ggtgtccatc gaggtcgcca
    29161 gcgccaccgc atatccgccg acgtcacgct gcacgccttt aggtttgccg gtcgtgccgg
    29221 aagtgtagag aatgcaggag gtttcgttgg attccagcca cgccaccggt acccgcgcgc
    29281 cgatgtgttg atggcgcaac gacgcgaaat cgacatcccg cccgctgacg cgcgccattt
    29341 tcgccagccc gcgatccacc agcaaaacat ggcgtggctg gtgctgcgcc tgacttatcg
    29401 catcgtcgag caatttttta tagggaatga ttttgccacc gcgcgctccg gcatcagccg
    29461 agacaatcag caccggttta gcgtcatcaa ttcgcgccgc cacgctgtgc gaggcaaaac
    29521 caccaaatac caccgaatga atagcaccaa tgcgcgcgca ggccagcaga gtaatatgcg
    29581 cttcggcaat catcggcata tacaccagca cccgatcgcc gcgctgcacg cccagtgagc
    29641 gcaacattga ggccaccgcg ttcacttcgt catgcagctg gcgaaaggta aaggtgcgct
    29701 cttcttctgt ttccgaagag acggcaatca atgccagtgc ctctggctgt ttctccagcc
    29761 agcggtcgat agcgttgtgg cacaaattgg ttcggccttc acaaaaccaa cgggcaaacg
    29821 gcggattgct gtggtcgagc gtttgcgtaa agggcgtctg ccagtcaata cgtcgggcct
    29881 gctcggccca gaactgctcc ggttcgttaa tcgaacgctg ataaaattcg ctaaaagaca
    29941 taatgctctc ctgttgaact tacgccttac cgccgtttac ttaaatgacg tacaggtcga
    30001 gatactcatt gaccggcatc tgttccaggc gagttctgtc gagagaaacc tccagaatgc
    30061 gctgctgttg gcgagtcggg aactggcgcg cgagattgat tttgaattta tcgaccagtt
    30121 tcggaatacc atcctgacgg cggcgagcat gaccaatggg gtactccacc accacttctt
    30181 caaatcgtgt gccgtcggtg aactcaaggg taatggcatt ggcgatggcg cgtttttccg
    30241 ggtcgtggta gtcagcggta aatgccggat cttcaaagca attgatcttc tcgcgcaggg
    30301 cgtcgatgcg tttatcttgc gcaacgttgt cctcgtaatc tgccgccgtt aagcgcccga
    30361 ataacagcgg gatcgccacc atgtactgaa tgcagtggtc gcggtcagcc gggttattga
    30421 gcggcccctt tttgtcgatg atgcgaatac aggcttcgtg ggtgcgaatg gtcacttttt
    30481 cgatatccgc cgccgtcttg cctgctgcct gcatctgttc atagagcgtc atcgccgctt
    30541 caactgccgt ctgggagtgg aactccgccg ggaaggagat tttgaacagc acattctcca
    30601 tgacgtaaga accgtacgga cgctggaagc ggaacgattc acctttaaag gagacgtcgt
    30661 agaagcccca taccggcgcg gtcagggctg acgggtaacc catttcgccc gttttcgcca
    30721 tcagtgccag acgtaccgcg cgggaagtgg catcgcccgc cgcccaggat ttacgcgtgc
    30781 cggtgttcgg cgcatggcga taggtgcgca gcgactgacc gtccacccac gccagcgaaa
    30841 cggcgttgag aatttcctcg cgggtcaggc cgagcatttc ggcgaccacg gcggtggaag
    30901 ccactttcac taacagaacg tggtcgaggc cgacgcggtt aaaggagttt tccagcgcga
    30961 tgcagccctg aatttcatgg gctttgatca ttgcggtcag cacctgtttc atggtcaacg
    31021 gcgctttgcc gctggcgacc gcgttgcgcg aaagccagtc tgccgttgcc agaatgccgc
    31081 cgaggttgtc ggaaggatgc ccccactccg ccgccagcca ggtatcgttg aaatcgagcc
    31141 agcggatcat cgcgccaatg ttaaatgccg cctggacggg gtcgagctga aactgagttc
    31201 cggggacgcg cacgccgttg ggtacgacgg tgccaggaac aattggcccc agcagttttt
    31261 tacaggccgg gtattcgaga gcttcaagac cgcagccgag cgtgtcgagc aggcagtaat
    31321 gtgcggtgtc gtaggccact ttagagctga tttcgtagtt catcacgtaa tcgacgatat
    31381 caacgatttc acgatcaaat tccgggcgga tgttgttgat ttgagctgac attgggtacg
    31441 tttccttatt gttattcgta gaggtttact ggcgcttatc cagcgcgaca aactggcggt
    31501 cttccggtcc aacataattg gcggaaggac ggataatttt gttgtcctga cgttgttcaa
    31561 tgatgtgcgc cgcccagccg gtgacacggg cgatgacaaa cagtggtgtg aacatctcgg
    31621 tgggaacgcc catcatgttg taggaaacag cagagaacca gtcgagattg gggaacatct
    31681 ttttgctctc ccacatcacc gtttccaggc gatcggcgat gttgtacatc ttcaacgagc
    31741 cgccttcctg cgagagctgc ttcgccacac gtttgatcac ctggtggcgc gggtcggcga
    31801 tggtataaac cggatgacca aaaccaatga ccacttcttt gttttccacc cgcttgcgga
    31861 tatcggcttc ggcttcgtcc ggcgtttcgt agcgttgctg gatctccagc gacacttcat
    31921 tcgctccgcc gtgtttcggc ccgcgcagtg cgccaatcgc gccaataatc gcggaataca
    31981 tatcagagcc agtgcccgca atcacccgac tggtaaaggt ggaggcgtta aactcgtgtt
    32041 cggcgtacag cactagcgaa atatgcatcg ccttttccca gctttgcgat ggcttttcgc
    32101 cgtgcagcag atgcaggaag tgaccaccga tggagtcgtc atcggtttcc ggctggatgc
    32161 gttcgccgtt gtgactgtag tgataccaat aaaggagaat cgagctaagt gatgccagta
    32221 gtttgtcggc aatatcccgc gcgccagaga cggtatgtcc ctctttttct ggcagcgtgc
    32281 agccgagcgc ggaaacgccg gtgcgcataa catccatcgg gtgtgacgcc gccggtaagg
    32341 cttccagcac ggtacgcacg ttagccggta aaccgcgcag ggctttcagt ttcgttttgt
    32401 aagcggcgag ttcgtcacgg gtcggcagtt tgccgtggat cagcagatgc gccacttctt
    32461 caaattcgca atgttccgcc agatcaagaa tatcgtagcc gcggtaatgc aggtcattgc
    32521 cgcttttacc cacggtgcag agcgccgtat tgcccgccgg aacgccagaa agtgccacag
    32581 attttttcgg tttaatgaca tgggtactgt tttgcaggat cgttgtgtcg ctcatgttgt
    32641 cctcgtcatt tgtagggtcg agatattttt cgttgtgttt tatttcacct ggctacgggc
    32701 aaacaggtcg tcgagcttcg cttcgtactg gtagtagttg atgctttcgt acagctcgtt
    32761 gcgggtttgc atggtatcga tgacgctttt ctgcgtgcct tcctggcgca ggacgttgta
    32821 gacatgctca gcggcgcggt tcatggcgcg gaacgctgaa agtgggtaca gcgccattgc
    32881 gacatgggcg ctgcgtaatt cgtcggtggt aaacagcggt gttgcgccaa attcggtaat
    32941 gttggcgagg atcggcacct gcaccgcatc ggcaaactgg cggtacatgg cgagttcggt
    33001 aatcgcctcc gggaacaaca tctcggcacc cgcttcaaca taggcctgtg cacgctcgat
    33061 cgccgcatcc agcccctcta ccgccagcgc atcggtgcgc gccatgatca caaaatcagg
    33121 atcggttttc gcatccaccg ccgcgcggat ccgatccacc atctcctctt tcgagacgat
    33181 cgctttgttc gggcgatgac cgcagcgttt cgcaccaacc tgatcttcaa tatgcaatcc
    33241 tgccgcaccg gctttaatca tcgatttcac cgtacgcgcc acgttaaagg ccgaagaacc
    33301 aaaaccgata tccgcatcca ccagcagtgg cagcgaacaa acgtcggtga tacggcgaat
    33361 atctgtcagc acatcatcaa gggtagaaat accgagatcg ggcagcccca gcgaccctgc
    33421 cgccacgcca ccgccggaga gataaattgc ctgatatccg gcacgctgcg ccaacagcgc
    33481 atgattagcg ttgatggtgc caacaatctg caatggattt tctttagtca gtgcagcgcg
    33541 aaacgcttta cctggagagt gtagagacat agcccatcct ttgttatcaa cttgttattt
    33601 tcgttaataa aacaaagcaa ggggtgtgcc acaattgcct taataaaatt aaatgtttaa
    33661 tttcaatttg ttagagataa cgtcatgaac taatccgcaa atatgcgttt cagttaacgt
    33721 ttcaggcaat gtttcaccgc gtttcattgc aacaattatg aaacaagact aaacccaata
    33781 ttcggtttct taactttgcg gtgcgctatg gcacatccac cacggcttaa tgacgacaaa
    33841 ccggttatct ggacggtatc tgtaacgcgc ctgttcgagc tgtttcgcga tatcagcctc
    33901 gagtttgatc acctggcgaa cattacccct attcagcttg gctttgaaaa agcagtgacc
    33961 tacatccgca agaaactggc aaacgaacgc tgtgacgcca tcatcgctgc tggctctaac
    34021 ggcgcgtacc tgaaaagccg cctgtcagtg ccagttattt tgattaaacc gagcggctac
    34081 gatgtgttac aggcactggc aaaagccgga aaactcacct cttctatcgg cgttgtcacc
    34141 tatcaggaaa ccattccagc gctggtggcg tttcaaaaaa cctttaatct gcgcctcgat
    34201 caacgtagct acattaccga agaagacgcc cgcgggcaga ttaacgagct gaaagctaac
    34261 ggcactgaag cggtggtcgg cgcggggctg attaccgatc tggcagaaga agccggaatg
    34321 accggaattt ttatctattc cgccgccacc gtgcgccagg cgttcagcga tgcgctggat
    34381 atgacgcgca tgtcgttacg ccataacact cacgatgcca cccgcaacgc cctgagaact
    34441 cgttacgtgc tgggcgatat gctcggtcaa tcaccacaga tggaacaagt acggcagact
    34501 attttgctgt atgcccgctc cagtgcagcg gtgttgattg agggggaaac ggggacgggc
    34561 aaagagctgg cggcccaggc gattcatcgg gaatattttg cccgccacga tgcgcgacag
    34621 ggcaaaaagt cgcatccgtt tgttgccgtc aactgcgggg cgatagccga atcgctgctg
    34681 gaagcggagc tgtttggcta tgaggaaggg gcgtttaccg gctcgcgacg cggaggtcgc
    34741 gccgggctgt tcgaaattgc ccacggcggt acgctgtttc tggatgagat tggcgaaatg
    34801 ccgctacctt tgcagactcg cctgttacgg gtgctggaag aaaaagaggt cacccgcgtc
    34861 ggcgggcatc agcctgttcc ggtagatgta cgggtcatta gcgccactca ctgcaagctg
    34921 gaagaagata tgcagcaagg acgttttcgc cgtgatctgt tttatcggct gagtattttg
    34981 cgtctgcaat tgccaccact gcgcgagcgg gtggcggata ttcttccgct ggcggaaagc
    35041 tttttgaaag tgtctctggc ggcgctctcc gccccgtttt ctgccgcatt acgccaggga
    35101 ttacaggcaa gcgaaaccgt gctggtgcac tacgactggc caggtaatat tcgtgaactg
    35161 cgcaatatga tggagcgact ggcgctattt ttaagtgtgg aaccgacgcc ggatttaacg
    35221 ccgcaatttt tgcagctgct actgccggaa ctggcgcgcg agtcggcgaa aactcccgct
    35281 ccacgcttac tgacaccaca acaggcactg gagaaattta atggcgataa aacagcagcg
    35341 gcgaattatt taggcatcag ccggacgacg ttctggcggc ggctgaaaag ctgaactgcc
    35401 gaatgcagcg cgaacgcttt atccggcata aatttcaata gatgatcacc gataaactta
    35461 cgcatcgggc atgtttacat agggttcaga attacttctt cttataaata tttgccgtgc
    35521 cgtggatctt attgtcagtt tgaccggagg tcagtaccaa cacatcagcc cctttttcat
    35581 ccgctttttt gatcaaatct tcttttgcat ctgcagtcga catttcattg ctggttgaaa
    35641 tatcacctat tttttcatac tgcgattcaa ctttttcaaa ttccgctttg gtcatcaatt
    35701 cagcggcata gacattggta acggctaacg ctaacgcacc gactaacatt ttagagatta
    35761 ttttcatatt acggctccag agttgttatc acactatcat ccacccacat tattgggttg
    35821 attaataatt agttcagtca gggcataaac gccagtaacc ggaaataaga cactttgtga
    35881 tctctctcgc acccgattat ttttggtaga aatatgatga agttacttca cttatttatg
    35941 gatgaaatca ctatggatcc tttgcacgcc gtttacctga ccgtaggact gttcgtgatt
    36001 acttttttta atccgggagc caatctcttt gtggtagtac aaaccagcct ggcttccggt
    36061 cgacgcgcag gggtgctgac cgggctgggc gtggcgctgg gcgatgcatt ttattccggg
    36121 ttgggtttgt ttggtcttgc aacgctaatt acgcagtgtg aggagatttt ttcgcttatc
    36181 agaatcgtcg gcggcgctta tctcttatgg tttgcgtggt gcagcatgcg ccgccagtca
    36241 acaccgcaaa tgagcacact acaacaaccg attagcgccc cctggtatgt cttttttcgc
    36301 cgcggattaa ttaccgatct ctctaacccg caaaccgttt tattttttat cagtattttc
    36361 tcagtaacat taaatgccga aacaccaaca tgggcacgtt taatggcctg ggcggggatt
    36421 gtgctcgcat caattatctg gcgagttttt cttagtcagg cgttttcttt gcccgctgtg
    36481 cgtcgtgctt atgggcgtat gcaacgcgtt gccagtcggg ttattggtgc aattattggt
    36541 gtattcgcgc tacgcctgat ttacgaaggg gtgacgcagc ggtgagcaga taatttactc
    36601 tctacgtaat caacctgatt tgccgaatcc cgaccatcgg cgatgatagc ccccgtaatg
    36661 cgtggtcctt atttccgata agtttacttg agtctttagc ttcccatttc agcccactct
    36721 cccctgtgtg ggcttttttc attggtctga cccgtcctga atagccaaac aatggcaatg
    36781 ttccactttg cagggatgat aataagggga tctgattcag aagagttttg aaaagttgga
    36841 ccgccatcga ggcctcgaac cccgtaccat acaacaatcc tggttgtcgt ctgctcttcc
    36901 tgatgagcta atggcggttt ttgatatgag ccttacagca tattttctgt caggcttgag
    36961 tgcgatgcgt aaggtacaga aaagacccca tacaaacatg atggaattat gatttttttc
    37021 atatgaatgt caacccacac attcacaaaa catctgcatg agatcatttt ttatacacca
    37081 catagctgcg aaaccactgc tggtgctttt ttaagcgaag cgtcgctgat cacaacaaaa
    37141 tatcggcgaa aaggcggtgg aatgccattt agcatacgga tatacagatg aaatgattac
    37201 ctgctttgcg attcactctc tggtggaata acaacttgca ggtggaaccc tttctgaact
    37261 aaagagtgta gcggatttta acgtgcttat ttacaggata atatctgtca cctaattcct
    37321 gcactctatt cgatatccat tcatttaatc gtgcgtcatc tctgtatgtt tttacgtcca
    37381 gaacagagag caataacatc accagtgatt caggaaaaac agggtagata ttttttaagt
    37441 gtaataataa tccatgcgca agcgcctgct taatgggcgc gtctgtctcc ggaatagacg
    37501 acatttcgcg aagccatttt tccacatgca tacactcttt ccctttaggg aaaagtctca
    37561 gcaaaggcat atggtgttca agaagaatac atttgcaatc tgttcgcact gaatcgataa
    37621 actgaactaa tttcttaagc ccggtaaagc gtccagcctg cacatttgct ttaatatcaa
    37681 gaaacgcatc atcctggtca tatacccaaa ctgtcccttt ttgattttca agtatgcgaa
    37741 aaacaaactt aaccgggtga tcagggtaat ccatccagtc ataaaatcca gggaaatcat
    37801 taaactgcac atttatggtg agtgcgttat cgtctgacca ctcacagtct cgtacggcag
    37861 ccatgagatg aatgagtttg tcgtcatcga ctaaatcatc acgacaatca taaggataat
    37921 cataatattg tttaatcaaa accttgaata tatcgacaca ggaaaccagg gagcttttga
    37981 tatcctctgg taactgattg tttttaaccg tattaagtat gctttgttgc gtatatggca
    38041 tcaaattatt gtgcggagct tttaatgata tcatcgcgat ctctcttaaa taaaaataaa
    38101 cgtcgcagat aataaagctc tgattaaacc aggcactatc agaaatcgct cataactaac
    38161 gtgtgaagta ttgtgtactg gagggagtta atttaacaat ttgaatttac gttataggtt
    38221 ttactgctac tttgatatta tttcgctctc cttaactaaa aagttaagtg gagagcgaag
    38281 ttaatatgtt aaaccacagc gtatttatta attttttcag tctgttagtg tgcgattatc
    38341 gataacaaaa cgatatttca catcaccacg cagcattcgc tcataggctt cattaatttg
    38401 atcggcccga atcatctcta tatcagccac gatgccatgt tcggcgcaaa aatcgagcat
    38461 ctcctgagtt tctggaatgc cgccaatcat agaaccggct atcgcacggc gtttcatgat
    38521 caggttgaaa acttccggcg atttatgcgg tgtcgcaggc gcaccaacca gcgtcatggt
    38581 gccatcacgc ttcagcaagg tggtaaaatc gtcgagatta tgtggcgcag ctactgtatt
    38641 caaaatgaaa tcgaaactct tcagatgagc cgccatctca tcggcattgc gtgagttaac
    38701 aacttcatcg gcccccaggg cttttgccgc ttcgcgtttt gcctcagaag tggtaaatgc
    38761 caccacatgc gcccccatcg cgtgggccag cttgatcccc atatgtccca gaccgccgat
    38821 gccgaccacg cccacttttt tacccggccc ggcctgccag tgacgtagcg gcgaatacgt
    38881 ggtgatccct gcacacaaca aaggagccac cgccgccagc tgctcttgcg ggtgacgaat
    38941 acgcagaaca tatcgctcat gaacgacgat ctgttgtgag tagccgccca gagtatggcc
    39001 cggttcgtcc ggcgtcggcg agttataggt gccggtcatg tgatcacagt agttttccag
    39061 cccgtcttca cactcttcgc aatgtttaca actgtcgaca atgcagccga caccgaccag
    39121 atcgcccggc gcatgttttt ctacctgatc accaacggct accacacgcc ccacaatttc
    39181 atgacccggt acgcaggggt aaaccgtccc cgcccactcg gaacggacct ggtggagatc
    39241 ggaatggcag acgccacagt aagcgatttc gattttgaca tcattcggtc ccggttcacg
    39301 ccgggtgata tccatcggtt caagtggttg tttagcggaa tatgcaccaa cagctttgat
    39361 cttcattgtg tttactcctg attagctatg tgtatttggc gatgcctgat atgtcatcgc
    39421 ggcaaaacgc gtccattgaa tatagccaat atttaaacca cccgatccag ccccaacatg
    39481 ccgcgagcct gaatgaaaca aagcagcaac gttccccata aggcgagcac ctgtaagtgt
    39541 gatatttgct atccgtaaaa gactaaaatc ggcaaaactg atgatctttt cacgtagaca
    39601 aatagttatc tacacaacgt caataagggc atattcctca aagataatat ccatttctgc
    39661 cgacacactc atattcaggt tgccccactc cctggggctt aaatctctta ctattcaccg
    39721 ttgcaggcgt ttcaaatgcg catcaacctg ccacactccc ccacaccagt tgccctttat
    39781 ggaacgttgc ggttctcggc gagatacgcg ccaccgcctc ggcggaacag gaggcgtcca
    39841 ccagcacaaa actggcgtca tcctgcgctt tgggccacac gcgctcgcct ttttcgttga
    39901 gcggcaatac atcgccagtg gctaaaaaca gcgagcggga gaggttctgt tcgttaggac
    39961 gaatatagag ctgcgcgtac agattggctt tttccagcat gtcgcccagg ccgtaaggcg
    40021 accagtggtc gataacgctg tcagtgccag tcatcacttt tacgcctttg tcgtgcaact
    40081 gtttgagcgg catatgcagc gtgccaatcg gcaccgtcga ggcgatagaa atttgctgcg
    40141 ccgccatccg gtgcgccagt tcatctacct gttgttcgtt gagcgttgcc agcgcaaagg
    40201 cgtgactgat ggtcagcttg cccttcagct gtggcgtttt ctctaccgtt tcaaccatat
    40261 aattgatggc tgccacgcct gccggagtgg tttcatgcag gtgaatatcg acgcctttgt
    40321 cgtagtccag cgcaatctga aacatggtgt cgagggattt ttccatcgcg ccatcaacac
    40381 tggtcgggtc cagcccgccg acgtaatgcg cacccgcctg catcgcttcg cgcattaagg
    40441 gttccgattt cgacagcagc aaaccgtgct gcgggaaggc gacgatttca cactcgaagc
    40501 ccgcctgacg ccgcgccagc accgcctgca aattttgcag attttttagg ccggaaaccg
    40561 gttcgatatt gcagtggctg cgggcaatgg tggtgccttt cgactgcaat agatcgatca
    40621 gtttttccgc ccgctcctgc gtatacggtt gtaactccgg cagcattttc tgctcgagtt
    40681 tgatcatgtc ctggatagtg gtgcctgccg gacgattgag cgagcgccac ggcccgccgt
    40741 agaaggtttt gtcgagatga atatgcatgt cgcgagtggt gggtagcatc aacttaccgc
    40801 cagcgtcata gtgcggcagc gtggcgtccg gatgctgctt gttctcgcgc agggcgacaa
    40861 ttttgccgtc ctgaatctcc acggtctggc gcgccgtgcg ggtctgcacc gccacaccgt
    40921 tttcgtagtc aaaaccggtt tccagcagca cgttatcgag atagtaatgc gggtcagtga
    40981 ttttcatggt gttgttcgct tcgcagttta gggtgccagc taccgccgca tgggcgagcg
    41041 gcacagaggt accaaacagc gcggcggcgg tgaccatctt accgctctgg ctcagaaatt
    41101 cacggcggct attgctttct ttcatcctac gtccttacgt tttaataata tgggtgcccg
    41161 tttcaccgcg cagcgccgca ggcaggcatt ccggcgtggt gataatcact tctttgccgc
    41221 cctgctccag gaacgtcagg ctggcgatga tttttggcaa catgctgccg ggcggaaaat
    41281 gcccttcctg catatagcga gtcatggtgg caatatccac ccgatccagc gcctgctgct
    41341 gcggtttacc aaagtggata cagacttttt cgacgccggt ggtgatcacc agaatgtcgg
    41401 cgtgaatttc acgggccagc agcgcggtag agagatcttt gtcaataacc gcgtccacgc
    41461 tttggtaatc ccccgcttca gtacgcacta ccggaattcc accgccgccc gcgccaatta
    41521 cgacaaaacc ttgttggatc agcgccttaa tggcgggtgc ttcgacaata cgtttcggtt
    41581 ccggcgaggc gaccacgcgg cgatagcccc gtccggcatc ttcaacaaaa caccagtcag
    41641 ggtttgcctt ttgtagtttg tcacgctggc tttcactgaa gaatgcacca atgggcttgg
    41701 tgggatgggc aaaacctgga tcgtttttat ccacttccac ctgagttacc acggtgacgg
    41761 cttttttctc gccgtgacgc gccagccggt tattcagcgc ctgttggatc agatagccga
    41821 tgccgccctg cgtatctgcc acacagttcg ccagcggcgt taagggcagc ccttcgcgct
    41881 cgtgggcaat ctccgcacgg cgtaaatcca gcccgacctg cggcccgtta ccgtgggtca
    41941 gcacaatgtc gtaatcggaa gccagcattt ccagcaccgt atcggccacg gctttcaccg
    42001 cctccgcctg atgctcaatc gactggctgg cgttatcttt gataatgctg ttgccaccaa
    42061 tggcaacgac cacaagctct ttcatgttgt tatgtccgct acagagggtg attaggttat
    42121 gaccaacgcc agactgcctg atgcgctacg cttatcaggc ctacaattct cgggccatcc
    42181 gtaggccgga taaggcgttc acaccgcatc cggcataaac atgtaagtca gacgccgtaa
    42241 cgttcgcacc agccgagaat ggctttctcg aagcaggcga atggtggatg cacaatgccc
    42301 gcgccaatca tgccgacgcc cgcatcttta tgggcgatgg cggtgttgat caccggcaga
    42361 atgccgctgc tacccacgcg ggtgatgtcg attgccgacg gcacgcccat aaaaccgagc
    42421 agcggaatgg tgacgttggg gttttcgcca agggtgattt cgcgcatctg acgggagaaa
    42481 tcgatagcct cttccaccgt gccgcccacc agtgcgacga ttgcaggcgc ggtcgccatg
    42541 gcaaacccgc caataccgta ggtttctgtg atggcgctgt cgccgatatc cagccccgaa
    42601 tcttccggct tataaccagc aaacatcggg ccgatgacct gctgcgctgg gccggtaaac
    42661 cactgccccg gcaggccgct gacccgcagg ccgaactcga cgccgttacg cgccatggtg
    42721 gtgaccacgg tgctgtattc gatgccgtgc gccgcatcca tcgccgcttt acacatcgcc
    42781 atccacgtcg ggccggagaa gtagtcgctg ctggcgacaa actcgaacac ttcgcgctgt
    42841 tgctcgacgg aatagcctgc ctgaatgatc cccggcgtca gtgcctgaat cagcagcgtc
    42901 gtcccggcgt tattgcggtt atggcactca tcgcccatat gcagcgcctg cgccagcatt
    42961 aagcgcagat cgatctcacc gataattttc atcgcgtcgc gcagcattgg cccctgcaca
    43021 tcgcgcatcc agttgaggcg gtcgatcacg ctctgatcgt tggcacccat acgcaaaatc
    43081 ttcgccatct gctcgctcat attggtataa gcgatgttgc cgtaggtttt gtttttcacg
    43141 atgtgcataa acatcgaggc cgaggtaacg cccgccatcg atcccacgca gtcgtgttcg
    43201 tggcacggcg agaaggtgat ctccccggag gccgccagtt cagtcgcctc atcgagatct
    43261 ttcgccagcc cttcgaacac caacgccccg gtgaccgcgc ctttcatcgc gccgcacatt
    43321 ttttcccagg tgatcggcgg cccggcgtga agaatggttt tcggcgtcat gcccggcacc
    43381 acgttaatcg cctgatcaaa accaatcagc accggatgcg actggataat gcgctccagc
    43441 gcctgctggt tagcggcggc gattttgtct gccagcggcg aatcggcaat gttatccagc
    43501 gcctgcacca cctgcatatt gccctgcccc ggcggcgtcc agtcgagttg agtcacttct
    43561 acatgctgct ttttcaggtc atcgctaaac atggcgatac cgacgttaat aacgttcaac
    43621 ggttggctaa acagtgactg gctcatcagg cttcctcccc tttgcagata aattcacgcg
    43681 ccagcaatcc ggtattggtg ctgctgctcg ccagaatcac tccggcatca agcagcatct
    43741 ggctttgttg ttctaatgat ggcgtatcga gatcggtgcc cagcacatag gcgagaatga
    43801 tcaactcgcg tccttcggcg gcggcgatcg ctttcgcttc tttgatcgcc tcgatggtgg
    43861 agccgaccgg atcttcatgc gatccaaatc cgagcacaaa atccatcacg atcaccgcca
    43921 cttctggatc gcgcgcttct tcgatcaagc gactgatgcg gttggtgggg tcaatcattg
    43981 gatgcggctt gccgttggtg aagtcgtcat cgccaaagtc gaggaaggtg tgtttgatgc
    44041 tgcggttgat atcttgcagg cggaattcag gatcgggctg aatgttgctg tagacatcgc
    44101 catgtttttc catcaccgcg aacatggttt cgtcgcacag cgtgccgccg cagaacaggc
    44161 cacgtatgta tttctgctgc ggttgcagac gcgcacgcac atcagcaatc aacggctggt
    44221 taagcgtatg caggtcgaga ttttcctgtt tcacgccgga gagcatcacc gcttttagcg
    44281 ccgcctcttt gctgccgcgg gcaaactgta gcccctgctc atccaccggc gtttcaacgc
    44341 gaccgaggaa gcagaccacc accggcttgc ggcaggcgcg cgcgcgttcc agcactttac
    44401 gggccaccgc aggcgcgggc ggtttggaga taagcacaat gatttcagtt tgcggatcgt
    44461 tttccagcat cccgatggcg tcgagcatca tcaggccgcc gattttctcg ctcaggtcgc
    44521 gcccgccggt gccaatcagt tgtgaaacgc cgccaccaaa ttcatgaatg cggacgctca
    44581 gctcctggct gccggtgccg gatgcgccaa caataccgat gttaccgcga cgcacggcgt
    44641 taccaaagca gagcgccgcg ccgttgataa tcgccgtgcc gcagtccggc cccatcatca
    44701 gcagcccttt ttcatgggcc aattgtttga gcgccagttc atcttcaacc gagacgttat
    44761 cggaaaacag catcacgttg agatcgtttt gcagcgcctg acgcgcttcg cgagcggcaa
    44821 acagaccgtt gaccgaaatc accgccaggt tactttccgg gatatgcttt ttggcactgg
    44881 caatagtggc gtaacgcgcc tcgtgcgagc cgctttgcgc tttggtgttg aacagttctt
    44941 caatctccac cagcaactgc tcgttgtccg cacccgattt accattgatg acaatcatca
    45001 ggtcgccgtt tttcgcctgt tccagctccg gcgtcagcag tcctaaattt ttcagcacac
    45061 ctttgttcat ttcggtcgcc atcgccacaa atgcctgctc gacgccgtcg agtttatttg
    45121 cacgcgtgga gatagacatc agcgagacag aatcaaaata ggtattcggt ttaatgacta
    45181 ttttaactga cataattccc tccgtaggtt tggctcagcg cacaaccatc ggccatgcca
    45241 tacagcatgt cgcagccgga actggagcca atatgtttaa ttttttctat tgcctgagtt
    45301 gcgttcccag ggatgtcata aataatgttg tgaataaaac tatgaatatt ttcccggcaa
    45361 cgttgttgta atgcggcttc cagcgttata gcacttaata atgtggtatt atttctgccg
    45421 cgttgcagac cgagataaaa ttcctctttg tatttttccg ccggatgccc gggaataaat
    45481 aaaacaagcg ccagacctgt taaataatcg tcggctgatg gcgtaaggcc aatccctaac
    45541 cccatcatac tggcgaccgc tgaggcgata ttttgtttat cgtttacggc ctgaataact
    45601 gcccgtcgtt taatatgtaa ttctttattt aacgcctgat aaaacggatt atcgccgcga
    45661 taattaaaca gggtttcatt ttcccccagg tgctgatgaa taatgtcgtg ccactgttgc
    45721 caggcgataa ggtgaaaatt cacagcggtc atttgcaagg ttggggacag ccaacgccga
    45781 caacgactca tctctatatg aagatgttga ccaaccgtaa tgccttgctc gtcaaactga
    45841 accttatcac catgccggaa cagaccatta aagtgagtga gtgccaaccg acaactgttt
    45901 ggcgcattgt cgtactcttc acaaagcagc gtcagcaact gctggcgagc cgggagtgcg
    45961 atatttaccg ctctggcaaa gacctgttca acctgtcctt gcccccgctg cgccagaaaa
    46021 tccgcatccg cagttagcgc ccacacacac cgtctttctg gtgtgttgat agcaagcctc
    46081 ccggtttatg cacctgcggc aatcagtaac tgcgcaatct cttcaaagcc ccgttcccgc
    46141 gccagttcca gtggcgtttt gccatattta tcggtcagat gcgggctggc accgtgttcc
    46201 agcaataact ggacaatcgc ctgctgttta atcccgccat cattaagcac aatcgcttcc
    46261 agcagcggtg tccagccaac atggttggtc tggttaacgt taatttccgt atgcgccaaa
    46321 agctctttta caatacttaa atggcctttt tcacaggcag gcgtcaggcc gacaccgcca
    46381 aaacgggtga cgcaattaag gtctggttta gccggtaaaa taattcgtag cagcgttaaa
    46441 tcaccgttca gacagctcat taaaaaagga tttaaacatg tcaggtcttg tttattaata
    46501 tccgctccgg catcaattaa tgcctgaacg caagcatatt gctgatataa acttgccagc
    46561 gtaattgccg ttttcccctg gcgatcgcag gtattaatat cgacacccag cgcaagacag
    46621 gtttttactt tatcgatatc gccctgttgt gcagccaata aatagtcggc aggtagattt
    46681 tttatcgtca ttcacattcc cctggttttt gtggctgttt tcgatgaatt gagaatagca
    46741 acgataatct gataaaagaa cccgcttaaa tttaattcgc cgtatttcgc ctttttgttg
    46801 aaagatgttc aacaatcttt tcgcagtgtg cggtagtgca atatttgttt ctttgacccg
    46861 ataccctttc actcaatcag gttttatttc tctatagtct gcacatgtaa gagtagcttt
    46921 cgtccatgtc taaccagagg ggaacaagat gaacggactc acggcaacgg gtgtcaccgt
    46981 cggtatttgt gcagggcttt ggcagttagt gtcttcacat gtcgggctct ctcagggatg
    47041 ggaattatta ggaaccattg gcttcgtcgc cttttgcagt ttttatgccg caggcggcgg
    47101 taaatcgggg tttatcagaa gtctggcggt taattattcc gggatggtgt gggcattttt
    47161 cgccgcgctg gctgccggtt ggctggcctc ggtgagtggg atttctgggt tctgggcgag
    47221 cgtgattact accgtgcctt tctcagcggt agtggtctgg caagggcgtt tctggttact
    47281 gtcatttatt cccggtggct ttctcggcat gacgctgttt tttgccagcg ggatgaactg
    47341 gacggtgacg ttactgggtt ttctggcggg taactgcgtg gggataattt cagaatatgg
    47401 tggacaaaaa ttaagcgaag cgacgactaa acgcgacggg tattaaaagt tattcaaaaa
    47461 tgattatcta tctttaaccg atacctctac agtcatagct ggagaaattc atagtagata
    47521 atggcgagaa tggtttaatg aactcaattt ttaccgaaga aaacctgttg gcatttacta
    47581 ccgctgcgcg ttttggcagc ttcagtaaag ccgcagaaga gttgggttta accacttccg
    47641 ccattagcta caccattaag cgtatggaga cggggctgga tgtggtgctg ttcactcgca
    47701 gtacccgcag cattgagtta acggagtccg ggcgttattt tttccgcaaa gctaccgacc
    47761 tgctgaatga tttttatgcc atcaaacgta gcatagatac tatttcgcag ggcatagaag
    47821 cgcgtgtgcg catttgtatt aatcagctgc tttatacgcc caaacacacc gcgcgcttat
    47881 tacaggtgct gaaaaagcag tttcctacct gccagattac cgtgacgacc gaagtgtata
    47941 acggcgtctg ggatgcgatt attaataatc aggccaatat cgccatcggc gcacccgata
    48001 cactactgga cggcggcggt attgattaca ctgaaattgg cgcgattcgc tgggcttttg
    48061 ctatcgcccc ggatcacccg ttagcctttg tgccggaacc catcgccgaa agccaactgc
    48121 gtctctaccc taatattatg gtggaggaca ccgcacatac gattaataaa aaagtgggct
    48181 ggttgctgca tgggcaggag tcaattctgg tgccagattt caacaccaaa tgtcagtgcc
    48241 agattttagg tgaaggaatt ggttttttgc cggattacat ggtccgtgag gcgatggcgc
    48301 aatccttgct ggttacccga caaatccata acccgcgcca ggattcacgc atgttactcg
    48361 caacccagca ctctgccacc ggtcaggtga cgcagtggat taaaaagcaa tttgcgccaa
    48421 atggcatatt aacggggatc tatcaggatt tactgcatcg ggagaattaa tcgctgccaa
    48481 cctcccgatg cgctgtgaat agcggtttac atcaaccacc agctttcatt acccattcag
    48541 aaacaaattt attacccggt actggcgggg agtacaaata gccctgcaaa aagtgaacgc
    48601 ctttaccgat cattaaatcc gcctgctcct gggtttctac cccttccgct acgatactca
    48661 gaccaggctt acgcgccagt tcgacaatat tgtccacaat atgaccggag atttcgtcga
    48721 cactcgccat ttgcacaaat gacttatcga tcttaataaa atcgaccggg aacgcctgca
    48781 agtaacgata ggtcgcataa cccgtaccaa agtcatccag cgcaaaggta atgttgtgct
    48841 gatgaaggct gtcaaatatc gctctggctt ctggcgttac cggaataggg ttacgttccg
    48901 ttagctcgag aaccagtttg attttatcgt ttcctaattt cttgaccagg ttcaggcatt
    48961 ctttttcaaa tcccgcggcc aaaaaacaac ccgccgagac gttgatacca atatggaaat
    49021 tatccggcag caaatgtttt accggcatca gaatatccgc agtctgtttc atcagttggc
    49081 gggtcattat gacgataaga ccagatgact ccgccagagg aataaactga tccggtggga
    49141 taattcccgt ttgtggatgt tcccagcgga caagcacctc acagcccgtc agtaccccag
    49201 tctgcgcgca gaaaaccggt tggatccacg gtttgaattc gtggttttcc aacgccagac
    49261 tgatggcttc aggcgtgacg atatggtgat agtgattatc attaatatat ctgtgactat
    49321 tactccccgt ggcggatatg atctccggat tgtactgaaa gaaaatatcg cgccagaaag
    49381 taatatcgct ctgaatcccc agtttctcaa agagctgttt cttttgatgt gaaattgtct
    49441 ttgcactgcg atttctgtac tgtgatattt ctgttacaga cattccctgc atcagcagct
    49501 tgagtagcag tcgttctttc tcggatacgc taccaggcaa tgacaaatga accgttgtac
    49561 cgaactcttg cagaaaaaca cgaaaatcac atgaattcat gaacacacct ttatctttta
    49621 tcgctgttca gtatgagtga ttacctgtcg attcctgagc acacataagg ggtaaccata
    49681 aacatcagcg atgccatacg tataacatcc cacatcacta tcaagaagat tatctttacc
    49741 tctgatgctc gctcaaaaac ag
//
LOCUS       DAPHJE010000016        49678 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPHJE010000016 DAPHJE010000000
VERSION     DAPHJE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.016"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            178..804
                     /locus_tag="RH222_000971"
     CDS             178..804
                     /locus_tag="RH222_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV3796029.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            825..2927
                     /locus_tag="RH222_000972"
     CDS             825..2927
                     /locus_tag="RH222_000972"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV3796030.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2940..3578
                     /locus_tag="RH222_000973"
     CDS             2940..3578
                     /locus_tag="RH222_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV3796031.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3642..4310
                     /locus_tag="RH222_000974"
     CDS             3642..4310
                     /locus_tag="RH222_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV3796032.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4307..5092
                     /locus_tag="RH222_000975"
     CDS             4307..5092
                     /locus_tag="RH222_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV3796033.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGGLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5096..5908
                     /locus_tag="RH222_000976"
     CDS             5096..5908
                     /locus_tag="RH222_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796034.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5920..7524)
                     /locus_tag="RH222_000977"
     CDS             complement(5920..7524)
                     /locus_tag="RH222_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV3796035.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRAPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7650..7955)
                     /locus_tag="RH222_000978"
     CDS             complement(7650..7955)
                     /locus_tag="RH222_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV3796036.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8063..8139)
                     /locus_tag="RH222_000979"
     tRNA            complement(8063..8139)
                     /locus_tag="RH222_000979"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8103..8105),aa:Val,seq:gac)
     gene            complement(8144..8220)
                     /locus_tag="RH222_000980"
     tRNA            complement(8144..8220)
                     /locus_tag="RH222_000980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8184..8186),aa:Val,seq:gac)
     gene            8528..9784
                     /locus_tag="RH222_000981"
     CDS             8528..9784
                     /locus_tag="RH222_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3796037.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9825..11198)
                     /gene="mdtK"
                     /locus_tag="RH222_000982"
     CDS             complement(9825..11198)
                     /gene="mdtK"
                     /locus_tag="RH222_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV3796038.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11413..12054
                     /gene="ribE"
                     /locus_tag="RH222_000983"
     CDS             11413..12054
                     /gene="ribE"
                     /locus_tag="RH222_000983"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV3796039.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12094..13242)
                     /gene="cfa"
                     /locus_tag="RH222_000984"
     CDS             complement(12094..13242)
                     /gene="cfa"
                     /locus_tag="RH222_000984"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV3796040.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13533..14744)
                     /locus_tag="RH222_000985"
     CDS             complement(13533..14744)
                     /locus_tag="RH222_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV3796041.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14857..15789
                     /locus_tag="RH222_000986"
     CDS             14857..15789
                     /locus_tag="RH222_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3796042.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVSLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15786..16811)
                     /gene="purR"
                     /locus_tag="RH222_000987"
     CDS             complement(15786..16811)
                     /gene="purR"
                     /locus_tag="RH222_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV3796043.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17110..17199
                     /gene="ynhF"
                     /locus_tag="RH222_000988"
     CDS             17110..17199
                     /gene="ynhF"
                     /locus_tag="RH222_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV3796044.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17365..18535
                     /locus_tag="RH222_000989"
                     /pseudo
     CDS             17365..18535
                     /locus_tag="RH222_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(18770..19351)
                     /gene="sodB"
                     /locus_tag="RH222_000990"
     CDS             complement(18770..19351)
                     /gene="sodB"
                     /locus_tag="RH222_000990"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV3796045.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19479..20294)
                     /gene="mepH"
                     /locus_tag="RH222_000991"
     CDS             complement(19479..20294)
                     /gene="mepH"
                     /locus_tag="RH222_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV3796046.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20628..20975
                     /gene="grxD"
                     /locus_tag="RH222_000992"
     CDS             20628..20975
                     /gene="grxD"
                     /locus_tag="RH222_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV3796047.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21026..25642)
                     /locus_tag="RH222_000993"
     CDS             complement(21026..25642)
                     /locus_tag="RH222_000993"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV3796048.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25735..26382)
                     /gene="rnt"
                     /locus_tag="RH222_000994"
     CDS             complement(25735..26382)
                     /gene="rnt"
                     /locus_tag="RH222_000994"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV3796049.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            complement(26485..26892)
                     /gene="gloA"
                     /locus_tag="RH222_000995"
     CDS             complement(26485..26892)
                     /gene="gloA"
                     /locus_tag="RH222_000995"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV3796050.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26973..28070)
                     /gene="nemA"
                     /locus_tag="RH222_000996"
     CDS             complement(26973..28070)
                     /gene="nemA"
                     /locus_tag="RH222_000996"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV3796051.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAEIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28107..28706)
                     /gene="nemR"
                     /locus_tag="RH222_000997"
     CDS             complement(28107..28706)
                     /gene="nemR"
                     /locus_tag="RH222_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV3796052.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28809..29048
                     /locus_tag="RH222_000998"
     CDS             28809..29048
                     /locus_tag="RH222_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV3796053.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRADKPASSDEPEQPSLF"
     gene            29097..29993
                     /locus_tag="RH222_000999"
     CDS             29097..29993
                     /locus_tag="RH222_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV3796054.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSTEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30074..30595
                     /gene="sodC"
                     /locus_tag="RH222_001000"
     CDS             30074..30595
                     /gene="sodC"
                     /locus_tag="RH222_001000"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV3796055.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30596..32608)
                     /locus_tag="RH222_001001"
     CDS             complement(30596..32608)
                     /locus_tag="RH222_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3796056.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32608..33465)
                     /locus_tag="RH222_001002"
     CDS             complement(32608..33465)
                     /locus_tag="RH222_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3796057.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVKAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33468..33704)
                     /locus_tag="RH222_001003"
     CDS             complement(33468..33704)
                     /locus_tag="RH222_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV3796058.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33905..34339
                     /gene="slyA"
                     /locus_tag="RH222_001004"
     CDS             33905..34339
                     /gene="slyA"
                     /locus_tag="RH222_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV3796059.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34386..34853)
                     /gene="slyB"
                     /locus_tag="RH222_001005"
     CDS             complement(34386..34853)
                     /gene="slyB"
                     /locus_tag="RH222_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV3796060.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35127..36236
                     /gene="anmK"
                     /locus_tag="RH222_001006"
     CDS             35127..36236
                     /gene="anmK"
                     /locus_tag="RH222_001006"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV3796061.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36334..36663
                     /gene="mliC"
                     /locus_tag="RH222_001007"
     CDS             36334..36663
                     /gene="mliC"
                     /locus_tag="RH222_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV3796062.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36722..37378
                     /gene="pdxH"
                     /locus_tag="RH222_001008"
     CDS             36722..37378
                     /gene="pdxH"
                     /locus_tag="RH222_001008"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV3796063.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37507..38781
                     /gene="tyrS"
                     /locus_tag="RH222_001009"
     CDS             37507..38781
                     /gene="tyrS"
                     /locus_tag="RH222_001009"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV3796064.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38840..39703
                     /gene="pdxY"
                     /locus_tag="RH222_001010"
     CDS             38840..39703
                     /gene="pdxY"
                     /locus_tag="RH222_001010"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDV3796065.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39747..40352)
                     /gene="gstA"
                     /locus_tag="RH222_001011"
     CDS             complement(39747..40352)
                     /gene="gstA"
                     /locus_tag="RH222_001011"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV3796066.1"
                     /translation="MKLFYIPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGFEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40458..41960)
                     /gene="dtpA"
                     /locus_tag="RH222_001012"
     CDS             complement(40458..41960)
                     /gene="dtpA"
                     /locus_tag="RH222_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV3796067.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42571..43206)
                     /gene="nth"
                     /locus_tag="RH222_001013"
     CDS             complement(42571..43206)
                     /gene="nth"
                     /locus_tag="RH222_001013"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV3796068.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43206..43901)
                     /gene="rsxE"
                     /locus_tag="RH222_001014"
     CDS             complement(43206..43901)
                     /gene="rsxE"
                     /locus_tag="RH222_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV3796069.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(43905..44525)
                     /gene="rsxG"
                     /locus_tag="RH222_001015"
     CDS             complement(43905..44525)
                     /gene="rsxG"
                     /locus_tag="RH222_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV3796070.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44529..45587)
                     /gene="rsxD"
                     /locus_tag="RH222_001016"
     CDS             complement(44529..45587)
                     /gene="rsxD"
                     /locus_tag="RH222_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV3796071.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45588..47714)
                     /gene="rsxC"
                     /locus_tag="RH222_001017"
     CDS             complement(45588..47714)
                     /gene="rsxC"
                     /locus_tag="RH222_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV3796072.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(47707..48285)
                     /gene="rsxB"
                     /locus_tag="RH222_001018"
     CDS             complement(47707..48285)
                     /gene="rsxB"
                     /locus_tag="RH222_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV3796073.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48285..48866)
                     /gene="rsxA"
                     /locus_tag="RH222_001019"
     CDS             complement(48285..48866)
                     /gene="rsxA"
                     /locus_tag="RH222_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV3796074.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48943..49383)
                     /locus_tag="RH222_001020"
     CDS             complement(48943..49383)
                     /locus_tag="RH222_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV3796075.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49469..>49678)
                     /gene="ydgT"
                     /locus_tag="RH222_001021"
     CDS             complement(49469..>49678)
                     /gene="ydgT"
                     /locus_tag="RH222_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV3796076.1"
                     /translation="VQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHR
                     RAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT        12494 a        12375 c        12957 g        11852 t
ORIGIN      
        1 ttattttttc tcctaaccat aaaggattag tttatcggcg ttaaatcttt tctcatacac
       61 tatcgggtta gggaaaatat ctggtgttgg ggacagagtc agcagcgtat tagttgaatg
      121 cttaatatca ggctgctgaa ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg
      181 aacccggttg atcgtccact attagatatt ggcttaacgc gattagaatt cttgcgtata
      241 tcaggaaaag gccttgctgg tctaacaatt gcccctgcgt tactgtcact tttgggttgc
      301 aaacaggaag atattgatag cggcacagta gggttgataa atacacccaa aggggtgctg
      361 gtgacccagc gagcgcgatg cacgggttgc caccgctgtg aaatctcttg taccaacttc
      421 aacgatggct cagtagggac attcttctcc cgtatcaaaa tccatcgcaa ttatttcttt
      481 ggcgacaacg gggttggctc tggcggcggc ctgtatggcg atctcaacta taccgcggac
      541 acctgccgtc aatgcaaaga gccgcaatgc atgaacgtct gcccgattgg tgcgattacc
      601 tggcagcaga aagaaggctg tattaccgtc gatcataaac gctgtattgg ctgtagcgcc
      661 tgtactacgg catgcccgtg gatgatggcc accgtaaata ccgaaagtaa aaaatcctcg
      721 aaatgtgtgt tatgcggtga atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc
      781 gagtggaaag atattactgt gtgaatcttg caaaggaaaa aatcatggct aacggttgga
      841 caggtaatat attaagagtc aatctcacga caggaaatat taccctcgaa gattccagta
      901 agtttaaaag ttttgtcggt ggcatgggct tcggctacaa aattatgtat gacgaagtac
      961 cgccaggcac gaaacctttc gatgaagcga ataaattagt ctttgctacc ggcccattaa
     1021 ctggatctgg tgccccctgt agttctcgcg taaatatcac ctcactttct acttttacca
     1081 aaggaaattt agttgtcgat gcccatatgg gtggcttttt tgcagcgcaa atgaaattcg
     1141 ctggatacga cgtcattatt atcgaaggga aggcgaaatc accggtatgg ctgaagatta
     1201 aagatgacaa agttagcctg gaaaaagccg atttcttatg gggaaaaggg acgcgcgcaa
     1261 cgacggaaga aatttgtcga ttgaccagtc cggaaacctg tgtggcggct attggtcagg
     1321 ctggggaaaa ccttgttcct ctctctggca tgttgaatag ccgtaaccac agcggcggtg
     1381 cgggaactgg cgcaattatg ggttcgaaaa acctgaaagc gattgcggtt gaagggacga
     1441 aaggggtcaa cattgccgat cgtcaggaga tgaagcgtct caacgattac atgatgactg
     1501 aacttattgg tgcgaataac aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt
     1561 attcagatcc caagtcacgt tggacagcac gtaaagggct gttttggggc gcggctgaag
     1621 gtggtccgat tgaaacgggg gaaattccgc caggcaatca gaacacggtc ggctttcgta
     1681 cctataaatc cgtttttgac ttaggtccgg cggcagagaa atacacagta aaaatgagtg
     1741 gctgccactc ttgcccgatc cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt
     1801 ttggcgtgcc cagcacaggt ggtaacactt gtgtagccaa ctttgtccat accaccatct
     1861 tcccgaacgg gccgaaagat tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg
     1921 ttggtctgaa tttgttcgat gactacggcc tatggtgtaa ctacgggcag ctgcatcgcg
     1981 actttactta ttgttacagc aaaggggtgt tcaagcgtgt tctgccagct gaagagtatg
     2041 cggaaattca ctgggatcaa ctggaagcgg gtgacgttaa tttcattaaa gatttttact
     2101 accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca tatgccatcg
     2161 cagaacgctg gaatttgggt gaagagtact ggggctacgc aaaaaataaa ctctggtcgc
     2221 cgtttggcta tccggttcac catgccaatg aagcgtcagc gcaggtcggt tccattgtta
     2281 actgtatgtt caaccgtgac tgcatgacgc atacccatat caattttatt ggttcgggtt
     2341 tgccattgaa actgcagcgt gaagtggcga aagagctttt tggttctgaa gatgcctacg
     2401 atgaaaccaa aaactacacg ccaatcaatg acgcaaaaat taaatatgca aagtggtcgc
     2461 tgttgcgggt ctgtttgcat aacgccgtca ccctgtgcaa ctgggtctgg ccaatgaccg
     2521 tttcgccgct gaaaagccgt aattaccggg gcgatctggc gcttgaagcc aaattcttca
     2581 aagcgatcac cggcgaagag atgactcagg aaaaattaga tttagctgca gagcgtatct
     2641 ttacgttgca tcgtgcctac acggtaaaac tgatgcaaac caaagatatg cgtaatgaac
     2701 acgatcttat ctgttcctgg gtattcgaca aggatccgca gatcccggtc tttactgaag
     2761 gtactgacaa aatggatcgt gacgatatgc atgcttcgct cacgatgttc tacaaagaaa
     2821 tgggctggga cccacagctt ggttgtccga cccgcgaaac attgcagcgt ctggggctgg
     2881 aagatattgc cgccgatctg gcagcacaca atctactgcc tgtgtaagga atgggtgaga
     2941 tgaaccatcg ggatgaattg ccgttagcga aagtcagcga agttgacgag gcgaagcggc
     3001 aatggttaca ggggatgcgt caccccgttg atacggtaac cgaaccagaa ccggctgaaa
     3061 tcctggcaga gttcattcgc cagcattctg cggctgggca gctggtggcg cgcgccgtgt
     3121 ttctctcgcc accctattcg gttgcagaag aggagctgtc agttttgctg gaaagcatta
     3181 agcagaacgg agactacgcg gatatcgctt gtatgacggg aagccaggat gattactatt
     3241 actcaacgca ggccatgagt gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg
     3301 acatttgtcg cgccattgca cacgccgttc gttttgagtg ccagacgtat cctcgtcctt
     3361 acaaagtcgc catgctgatg caagcgccat attacttcca ggaagcgcag attgaagccg
     3421 cgatcgccgc gatggatgtt gcacctgaat atgccgatat acggcaggtg gagtcatcga
     3481 cggcggtgtt gtacttgttt agcgagcgat ttatgaccta tggcaaggct tatggcttat
     3541 gcgaatggtt cgaggtagag cagtttcaga acccctgaaa tttcagggag atgtcatgga
     3601 tagggtggac agttgcagcc actgcattag gggataatca gatgtccttc actcgacgca
     3661 aatttgttct ggggatggga acagtaatct tttttactgg ttctgcgtct tctctgttag
     3721 cgaacacgag gcaagaaaag gaagttcggt atgccatgat tcatgacgag tcaaggtgca
     3781 atggctgtaa tatttgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa
     3841 gccgtttatc gatagcacac attccagtca ccgataacga caacgaaacg cagtatcact
     3901 tttttcgcca gtcatgtcag cactgcgaag atgctccgtg catcgacgta tgcccgacag
     3961 gggcgtcgtg gcgtgatgag caggggatcg tgcgggtaga aaaatcgcag tgcatcggct
     4021 gcagttattg cattggcgct tgtccgtacc aggttcgcta ccttaaccct gtcactaaag
     4081 tggcagataa atgtgatttc tgcgctgagt cccgactggc aaaaggcttt ccgcccattt
     4141 gcgtcagcgc ctgcccggaa cacgcattga tctttggtcg cgaagatagc ccagaaattc
     4201 aggcgtggtt gcagcaaaat aaatactatc aatatcagct acctggtgcc gggaaaccgc
     4261 atctgtatcg ccggttcggt caacatttga ttaaaaagga aaatgtatga acccgtcgca
     4321 acatgctgaa cagtttcaga gccagttagc gaactatgtg ccacagttca ctcccgaatt
     4381 ttggccggtg tggttgatca ttgccggagt attgctggtt gggatgtggt tggtgctggg
     4441 gctgcatgcc ttgcttcgtg ctcgtggcgt gaagaaatca gtcaccgatt atggtgagaa
     4501 gatttatctt tactgcaaag cggtcagatt atggcactgg tcgaatgcgt tactctttgt
     4561 attgttgctg gcaagtgggc tgataaatca ctttgcgctg gtgggcgcaa ctgcggttaa
     4621 aagtctggtt gcggtgcatg aagtttgcgg atttttgtta ctcgcatgct ggctcggctt
     4681 tgtgctgatc aatgccgttg ggggtaatgg tcaccactat cgcattcgtc gtcagggggg
     4741 gctggagcga gcggcaaaac aaacgcgatt ttatttgttt ggcattatgc agggggaaga
     4801 acatcctttc ccggcaacaa cccagtctaa atttaatccc ttacagcagg tcgcctatgt
     4861 tggtgttatg tatggattgc tgccgttgtt actattgacg gggctgctgt gtctctatcc
     4921 gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg ttattgcagg cgcattttgc
     4981 tctggcattt ataagcctct tttttatctt cggtcatctt tatctttgca ccacggggcg
     5041 tacgccacac gaaaccttta aaagcatggt cgatggctat caccggcact aacgaatgat
     5101 cattacacga gcagacctgc gagaatggcg tatcggagcg gtgatgtatc gctggttcct
     5161 gcgccatttc ccacgcggcg gtagttatgc cgatattcat catgcactga ttgaggaagg
     5221 atatacggac tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc tggcggatga
     5281 aaacttcgcg catcaggaag tttcctctat gcaaaaactg gccacagacc ccggagagat
     5341 accattttgc agtcagtttg cccgcagcga cgatcatgct cgcatcggct gttgtgagga
     5401 taacgcccga atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg gttatagcgt
     5461 gcggattgga agtgtcggct ttaacagtca cattggcagc tccggtgaac gcgcacgggt
     5521 tgctgtaacc ggtaattctt cgcggataag cagcgctgga gatagtagtc ggattgcgaa
     5581 taccggaatg cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg ccagtaatgg
     5641 tgacctggtg caaatcgcca gctttggcgc aaacgccaga attgccaaca gcggcgacaa
     5701 tgtacacatc attgccagcg gtgaaaattc aacggtagtg agcacgggcg tggtcgactc
     5761 aattattctc gggccagggg gaagcgccgc actggcatat catgacggcg aaagagttcg
     5821 cttcgccgtc gccattgaag gtgaaaacaa tattcgaacc ggagtccgct atcgtcttaa
     5881 tgaacagcat cagtttgttg agtgctgata gctttacctt taatcaaacg aaagacgccg
     5941 acgagcacgg gatgcaggct ttacgaccgc ccgagccatc gcctcaccaa tttctgcaca
     6001 tgccgtaagt ccctgaacga aaggctggtc gtgcatcaac cagggcaacg cgccgaacgc
     6061 tacgcgtcca cgaatatctt cgggttgctg taacgtataa tcttcgccaa catcagggat
     6121 ttcatcccct gttttctgta attgctcgcg tagcccaggg aaaggaatat ctttcacttt
     6181 aagcggacgc tgtccgcggg catcaataaa aacgtcaaac gagtagctgt tgtcttccgt
     6241 tttcaggacg gtgcgcgact cattaatttc cattttgtag tcttcaccga gggcgagaat
     6301 atgaatgatt cccgcttcac gtaaggcaag taggcgacga atagactctg aagggattgc
     6361 cgcataatta tcgatgaaaa ccctggcaag gcctttactg aaccgtttat ggtcgtgttc
     6421 attcagatga ggaacaattt cctgtacggc ttcatgcagg cgcagaatga cataacgcca
     6481 gggaacagta tgtttttctc gtttattgcg ttcgacttcc tggagatttt tttctgccca
     6541 gtcaaatggg gcgcgttgtt tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt
     6601 caggctctct aaggctatgc gttggctcca gtctggatca gcaaacttga tttcctctac
     6661 tatcaatcta aatacccgat ccaaaaggcc ctcttcacct ttttgaatct cagcatttaa
     6721 tgcctgatcg gtgacgatgt gtaagggctc gtagggaata gggcaataga aatcggcttc
     6781 gggtaaaata cccgtgcgcg acatcaacgt aatatttagc ttttcacttg cgttatcgcg
     6841 gttaaaaacg acgtgttgtt tatcatcttc aatgaacgaa ccatgctgaa tagccactgc
     6901 cattgccgca tccagtccgc tcaaggatgt tcccataata cccacgttac acgcatcgac
     6961 ctttgcttcc atcaggcctg accacggact gggaaaatac gttctggttg cttcttcttc
     7021 atcaggccag acgtgacccg tggcaatcac cgctaaatca aacgtctcgc tgggtaaatc
     7081 ctgatttgta gcgagcatga cgccagcatt tgtaatttgc agatcggtaa cctggcatga
     7141 ttcataaaca gccactgcaa atttttgctg tcgtgcctgg tctactaatc gtaaaaattg
     7201 atcgcggaaa tattcgccca gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc
     7261 ttttttaacg ccataacgct ggagatggct ggcttcttgc ttttgtagcc attcgagata
     7321 cgtacaatta atcggcggta tttcaatact ggcaatattt gccagcatca tttttgagtt
     7381 ttcctcatca ctgtatggca tcccgacacc ggcctcgtca gcctgctcga agatagaaat
     7441 agaaagtgga gtttgttgct gtagaagcga gaataaggtg tagatccccg taggcccggc
     7501 acccacaata gcaatttttt tcattgttac ttacctgtac gcattctttc gaatttagtg
     7561 ttatcagata agtttggttg agatttttag aatttgagaa atttcagaca gggatgaaaa
     7621 gggcgctaac cgcccttgat taaattctgt caggcgagtt ttgcctgatt gatttgactc
     7681 agtggctcat tgacatcgaa aactttggcg acaacttcct caacgccgag atttttaagt
     7741 cgggcagtat gtttttccag ataggcaagg gcgctttttt catcagtaaa aagatagatc
     7801 ccaccggctt cgtggttctt ttcactttct gtccatacct tccacagaaa accaggttcc
     7861 tgattaatcg actcagcaag cggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca
     7921 ttgaaagcaa aatgaagttg taacaaagtt gccatagcga ttctccttct gataacgcat
     7981 acgaagacag tgtaaatctt ttaacgtatt ggatacagag aagaaacaga gggaaaaagg
     8041 agaagaccag attaagaaaa tctggtgcgt ccgagtggac tcgaaccacc gacccccacc
     8101 atgtcaaggt ggtgctctaa ccaactgagc tacggacgca ggatggtgcg ttcaattgga
     8161 ctcgaaccaa cgacccccac catgtcaagg tggtgctcta accaactgag ctatgaacgc
     8221 aacgttgtag gtgacaacgg ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc
     8281 aaaacgtaat tttctgcgct atttcgaccg tttgcagagc ttttaagcaa attggctata
     8341 ttttgttgat ttgcaagggc gatttttatt caggatcgca tttacatctg atacaaccca
     8401 ataattcgtg tatcagagtt taaacagagt tgtttgccac catctcaaat ctgtcgtgga
     8461 taaatcctcc gtagccttta cacttaggta gcaataaagg cacacagatg cagcaaagga
     8521 ataaatcatg ggatctgatg cgaaaaactt gatgagcgac gggaatgtgc aaattgttaa
     8581 gaccggcgag gtcattggcg cgacgcaact tactgaaggc gagttgattg ttgaagctgg
     8641 cggaagagcc gaaaataccg tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg
     8701 gatcgccaaa tgcacacagt acggtaacaa tggcacgcta tcggtcagcg acggtgccat
     8761 tgccacagat attgttcagt ccgagggagg cgcaattagt ctctctacgc tcgctacggt
     8821 taatggccgc catcccgaag gtgaattcag cgttgatcag ggttatgcct gcggtttgtt
     8881 gctggaaaat ggcggtaacc tgcgtgtact ggaaggacat cgcgcggaaa aaatcattct
     8941 cgatcaagag ggcggcctgt tggttaatgg gacaacctca gcggtcgtgg tagatgaagg
     9001 tggtgaattg ttggtgtatc caggtgggga agccagcaat tgtgagatta atcagggcgg
     9061 cgtttttatg ctggccggga aagccagtga tacgttgctt gctggtggca ccatgaataa
     9121 tctcggtggt gaagactctg acactattgt tgagaatgga tccatttatc gtctggggac
     9181 ggatggcctt cagctctaca gttccggtaa gacgcaaaac ctgtccgtga atgtgggtgg
     9241 tcgggctgaa gtgcatgccg gtacgctgga aaatgcggta atacaaggtg gaacagtgat
     9301 cctgttgtca cccaccagcg cggacgaaaa ttttgtcgta gaggaagatc gcgcaccggt
     9361 tgaactgacc gggagtgttg cattactgga cggcgcttca atgattattg gctatggcgc
     9421 agatctgcaa caatcaacga ttactgtaca gcagggcggt gtgttgattc tcgacggcag
     9481 tacggtaaaa ggtgacggtg tcacttttat tgttggtaac atcaatctga atggcggaaa
     9541 actgtggctg atcactggtg cggcaacgca tgtgcaactg aaagtgaaac gcctgcgcgg
     9601 agagggagcg atttgcctgc aaaccagtgc gaaagaaatc tcacctgact tcatcaatgt
     9661 gaaaggggaa gttaccgggg atatacacgt tgagataaca gatgccagtc ggcaaactct
     9721 gtgcaacgct ctgaaattac agccagacga agacgggatt ggcgcaacgc tccagcctgc
     9781 gtaaaaacac aggcgcaacc cgtgaaggtt gcgccttgtc tttattagcg ggatgctcgt
     9841 tgcagaatga taactgacgg cagacgttgc aggaaccgca tacgcagcat catcataatg
     9901 gctgccgacg tcaggccaat aataaagcct atccagaagc ctgctggccc cataggttca
     9961 acgaccagat cggtcagtgc cagaatatag ccgcttggca agcccagcac ccagtaagcc
    10021 gtaaaggtga tatagaaaat ggaacgcgta tctttataac cacgcaaaat cccactgcca
    10081 atcacctgga ttgagtcaga aatctgatat accgccgcca gcaacatcaa atgcgcagcc
    10141 agcgttacaa cctcgggatt gtcgttgtac aacagggcga tttgctcccg cagtgaaacc
    10201 gtgaatatgg ccgtcagggt tgccatacag acacccacca taagcccggt ccgcgcagcg
    10261 gtttgcgcat ccagcgttga gccctgaccc agacgataac ctacgcggat agttaccgct
    10321 gccgccagcg acattggaag cacaaacatc agtgaactaa agttcagggc aatctggtgt
    10381 cctgcgacat caacaatacc gagcggagac actaacagag ccacgacggc aaacagtgtc
    10441 acttcaaaga atagtgccag cgcaatcggc aaaccgagtt gaatcagtcg tttcataacc
    10501 gcaggatcgg gtttcgcggt gcctttttcg ttacgaatat cgcgcatgga gcgggcgcgt
    10561 ttaatgtaag aaaccatggc aaggaacatg acccaataca ccgccgcagt agccacgcca
    10621 caaccaacgc caccgagctc aggcataccg aaatgaccat aaataaagat atagttcacc
    10681 ggaatgttca ccagcaggcc gataaagccc attaccatac ccggcttggt ttttgccaga
    10741 ccttcacatt ggttacgggc aacctggaag aacagatagc ccggcgcgcc ccacaacaac
    10801 gcacgcagat aacccacggc tttgtccgcc agagccggat cgatgttttc catggagcgg
    10861 ataatgtaac ctgcattcca cagcacaagc ataatgagaa cggaaacaaa acctgccagc
    10921 cagaaacctt gtcgcacctg atgcgcaatg cgctcacgtc gaccggaacc atttaattgc
    10981 gcgataaccg gcgttaatgc cagcagcagt ccgtgaccaa agaggatcgc cggaagccag
    11041 atagaagtac cgatagcgac cgccgccatg tcggtggcac tatagccgcc cgccatcacg
    11101 gtatcgacaa aacccatcgc agtttgggcg atttgcgcga gaatcaccgg gattgctaat
    11161 gctaataaca gacgcgcttc actgatatac ttctgcacgt gaacaccttt tatttgtagt
    11221 tatatgagag actaaaaagc cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc
    11281 agtctattgt agcgaggatt tattaattct ccagtgaaaa aatagtcatt cttacccctt
    11341 cactggtaag ggcgtttgcc acctgctatt gtgctgaaca gaatgtctta actgatttca
    11401 ggagttgtaa gtatgtttac ggggattgta cagggcaccg caaaactggt gtcgattgac
    11461 gagaaaccaa attttcgtac gcatgtggtg gagttacccg accacatgct ggacggcctg
    11521 gaaacaggtg cttccgtggc gcataacggt tgctgcctga ccgtgacgga aattaacggc
    11581 aaccatgtca gttttgacct gatgaaagaa acgttacgca ttaccaatct tggcgattta
    11641 aaagtggggg attgggtaaa cgttgagcgt gcggcgaaat tcagtgatga aattggcgga
    11701 cacttaatgt caggtcatat tatgaccacc gctgaagtgg cgaaaatatt aacctcggaa
    11761 aataatcgcc agatctggtt taaagtccag gatagtcagt tgatgaaata tatcctgtac
    11821 aaaggattta ttggcatcga cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt
    11881 ttttgcgtcc atttaattcc ggaaactctg gaacgcacga ctcttgggaa gaaaaaactc
    11941 ggcgcacgcg tcaacattga aattgatcca caaactcagg cagtggtaga tacggtagaa
    12001 cgtgtgctgg cggcacgaga aaatgccatg aatcaaccag gtacagaagc ctgatctgaa
    12061 atcgcccccg gagagggggc gatagaatag cctttagcga gcgactcgaa ggccgttttc
    12121 aacaccgcgt gagaacacga cctgccagag ctgaatatca cgggcgcgga aagcacctgc
    12181 acaggcattc agataatagg taaacattcg tttaaagcgt tcactgtagt tatccgcaat
    12241 ttctggccat gcggcgagga atcgttcata ccacgccatc aacgtagtat cgtaatcagc
    12301 accgaagtta tgccagtctt ccatcacgaa gtggggttcg ctggattgag caatctggcg
    12361 tacagagggc aggcaaccgt tcggaaaaat atatttatta atccagggat caacattcag
    12421 atcggttttt ttcgaaccga tagtatggag caggaatata ccttccggtt tcaaattacg
    12481 atccaccacc gcaaaatagg tatcgtaatt tttcggtccg acgtgctcga acatccccac
    12541 agaaacaata cgatcaaact ggtcgttcag gtcacgataa tcttgcagca aaatggtgac
    12601 atccaggcct tcacagcgtt cctgagccat tttttgctgt tcggcagaaa tggtgacgcc
    12661 gaccacgctt acgtcatagt tagatgccat gtagtgcgcc agtccgcccc agccgcagcc
    12721 aatatccagt acgcgcatcc ctggttttaa ctgcaatttt tcacaaatca ttttgagctt
    12781 cgcctgctgg gcagattcca gattatcggc atctttccag taagcgcagg aatattgcat
    12841 gaagggatca agcatgcggc tgaacaagtc attacccaaa tcgtaatgct ccttgccgac
    12901 tatccaggca cgttttttac tctgcagatt gaagagacga gcgctggcaa tacgcagcgt
    12961 gtctttgaaa tgatggggga gttggttctc gagacctgcg cgtaagactt tgctaaaaaa
    13021 catatccagt cggtcacatt cccaccagcc atccatataa ctttcgccta accccaaaga
    13081 gccttcttgc agaacgcgtt taaaaaaatc ggggtttttc acacgaatat ccgccggggc
    13141 agaaccgtta atggctatac cggcacggct aagtaattcg ttggcgatac ggtaccagtt
    13201 gtcatccggt acactgactt cttctataca cgatgaactc atagtttctc catcactggt
    13261 cggtgatcag aaccttaaaa cagcgtagac gctttttggg ctttgtgaga aatctcgcag
    13321 aaaaaaccgc gagtctataa tccgacgtag aacagaggga aaggaggaac ctttgccgaa
    13381 atgaccatcc atagtgagtc gggaacgatc ctgtccccgt aaaaacataa tatttatcgt
    13441 agctttaaca actcgaaatt agtataggcc gctaatttca atcatacaac aaaattttgt
    13501 tagcagccta agtataagta tatcgatata gatcagtgtg attcgctatg agcgacttcg
    13561 gcattgccat gattctggca gccaacttct tcacaacgtt gcatcatgta acccagagcg
    13621 accagcacta ctgttgataa catcacgctg gtggtggtga gcaatggcgt gctgatactg
    13681 atcagccagg aaactaccag acttgcgagg aagcacagac ccagttgtag agtgttctgc
    13741 aacgctgcgg cgcgaccagt tgcgtgtggg aagggacgca gcgcctgggc gacaacaatc
    13801 gggtagatcg cgccattggc aatcgccatc acacagaatg ggatcaggat ttcgaccagc
    13861 gacacatggc taatgaagcc tgcagcccag gtcgcaatga cgctgactgc aaacagcacc
    13921 agcaaccacg gtaataactg cttgccttgc catttctgca gcgcggcgcg acagccataa
    13981 ccaccaatca gaaacgcgat agtttgcggg acataactta aaccaataac tgccgggctg
    14041 tagcccattt cactaaggat gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa
    14101 caggccgcgt atatcagcac gttgccgcga taggttttag aacgtagcag gtcggtaaag
    14161 gtcagaccat cctgactatt gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc
    14221 agaatcagca ccacggtaat ggcaaacagg gtggcgaaaa tcgcctgcca ggaaaaatgg
    14281 accagcagcc agcttcctaa cagaggagcc agggccggag atagacccac cagcggcatg
    14341 atggtcgcaa aaattcggtt aactttctgt gaaggataat aatccgtcac taacgcttgc
    14401 cagataaccg ccgcggcgca gacacccaca gcctgtacaa aacgcaatac cagcagcgta
    14461 gcggcgtttt ctacccacag catccccaga ctacctaacg caaaaattgt caggccgatt
    14521 aataacaccg gtttacgacc ataacggtcg gagagtggcc cccacagaag ctgggctgcg
    14581 gcaaaaccgg caaggaacag actaaggctg gcactgacag cagacgcagg cgtttgcagg
    14641 tcggcctgta tggcggcgaa agcaggcaga tacatatcgg ttgccagaaa accgagtacg
    14701 ctcaaacccg ccagccagac taaaaatctt ttcccaggtt gcatgtatat tctctaaatc
    14761 tctcaattaa acaccgccgc aaagtgtagg gagtgaaatt agtgttgtga aacggtaata
    14821 tttgatgact ggtttcaaaa attttgcagg cagactatgt ggtcagaata ttcactcgaa
    14881 gttgttgatg cggtagcgcg taatggtagt tttagcgctg cggcacagga gctgcatcgc
    14941 gttccttctg cggtcagcta taccgtgcgt cagctggaag agtggctggc ggtgtcgctc
    15001 tttgaacgac ggcaccgtga tgtggagctg accgctgctg gagcgtggtt tctcaaagaa
    15061 gggcgctctg ttgtcaaaaa aatgcagatc acccgccagc aatgtcagca gatagcgaac
    15121 ggctggcgcg gtcagttagc tatcgcagtg gataatattg tcaggccaga acgtacacgg
    15181 cagatgatcg ttgattttta tcgccatttt gatgatgtcg aacttcttgt ttttcaggaa
    15241 gtgttcaacg gtgtctggga tgcgctttcc gacgggcgcg tggaactggc gattggcgca
    15301 acacgcgcga ttccggtagg cggtcgttat gccttccggg atatggggat gctaagctgg
    15361 agttgcgttg ttgccagcca ccacccgctg gcgttgatgg atggcccgtt cagcgatgat
    15421 acgttgcgca actggccgtc gttggtgcgc gaagacacct cgcgaacgct acctaaacgt
    15481 attacctggt tgctggataa ccaaaaaaga gttgtcgtgc ctgactggga atcatcggca
    15541 acctgtatct cggcaggatt atgcataggg atggtgccaa cacatttcgc caaaccgtgg
    15601 cttaatgagg ggaagtgggt ggcattagaa ctggaaaacc cctttccgga ttcggcatgt
    15661 tgtctgacat ggcagcaaaa tgatatgtcg cctgcgttaa cctggctgct tgagtatctc
    15721 ggcgatagtg agacgctgaa taaggagtgg ctgcgggagc cggaagagac tcccgcaacg
    15781 ggtgattaac gacgatagtc gcggaacggg ccgtcagcca cggagcggcg ttcaatcaag
    15841 cgcggatgca cttcaatgga ctgcggttct tcacgtttgt tgacgatacg atccaacagc
    15901 atgttgaacg ctgtttcacc cagcgaatct tttggctggt ggatcgtggt cagcgccggc
    15961 gtaaaatagc gggcgttgcg cacgttatca taaccgatca gcgaaacatc ctgcgggacg
    16021 cgcagaccca tttcatcagc agcacaaagt gcgcccattg ccatgatatc gccaccacag
    16081 aagacggcag tagggcgatg cggctgcgac agtatttgct gcatggcgcg ataaccagat
    16141 tcgggttcaa agtcaccctg aacaatccag ctttccggca ccttgatcat cgcttcttcc
    16201 atcgccttca taaaaccggc aaggcggcct gcgccggtgt tacgttccag cgggccgggg
    16261 atgacgccga tttcgcggtg accgcgttca atcagataac gcccggccat gtagccgcct
    16321 tcgaacgcgt tatcaatgac cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc
    16381 accaccattg ggatatggcg atactcttcc agcatcgcca gcaacggctc tgggtactca
    16441 gaacacatca ccagcagacc atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc
    16501 tgtttctcaa gattgttcca cgcattaccc agaatcaggg tgtaaccttt ctggaagcaa
    16561 tttttttcaa ccgcttcaat gatctcggca aaataggccg cttcgctgct ggtcgccagc
    16621 aaaccgatag acttggtgtg gttaaccttc aggctacgcg ccaccgcgct aggggagtag
    16681 tgtaattctt taatcgctgc ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt
    16741 ttgttgatca cgtgtgacac agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt
    16801 attgttgcca ttccatttca ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta
    16861 aacgtttgcc tttacacacc ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga
    16921 cacggagcgt caggaagggg tcaatggcca gtacgcaaat agtgtaagcg tggaattttg
    16981 gccgatcttg acgaaaaggg gaagtggtaa aaacacttat cattataaat gacgggagat
    17041 tttttcatca cagtgtgtaa aaatgcgtaa tatcactttt tgtgtggtaa taaaaaagga
    17101 gaaaacttga tgagcaccga ccttaaattt tcactggtaa caacgattat cgtcctcggt
    17161 ttgatcgtag ccgtgggttt gactgccgcg ctgcactgat ttcctctcga gggagcgcgt
    17221 tctccctcgc atctccttcc tgtttgttag caaaatggca aaagtgtttt ctctttttgt
    17281 tattttcatt tttttgtgat tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat
    17341 gacgcgaaga ggtggagata aggcatgaaa attaactatc cgttgctggc gctggcgatt
    17401 ggcgcgtttg gtatcgggac aacggagttc tcgccaatgg gcttgttgcc cgtcattgcg
    17461 cgcggtgtgg atgtctcgat tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc
    17521 gtaatggtgg gcgcgccgct gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg
    17581 ttgattttcc tgatggcaat tttcacgcta ggcaacgttc tttccgccat cgcgccggat
    17641 tatatgaccc tgatgctttc acgcattttg accagcctga atcacggagc attttttggt
    17701 ttgggttcag tcgtggccgc aagcgtggtg ccaaaacata aacaggccag cgcagttgcc
    17761 actatgttta tggggttaac cctggcaaat atcggtggcg tgccggcggc gacctggttg
    17821 ggtgaaacca tcggctggcg gatgtcattt ctggcaacgg cggggctggg agtgatttca
    17881 atggtaagtc tgttcttctc attacctaaa ggtggtgcag gggcacgacc tgaagtgaaa
    17941 aaagagctgg cggtattaat gcgtccgcag gtgctgtctg cattgctgac gacggtactg
    18001 ggagctggtg caatgtttac tctctacacc tatatctctc cggtactgca aagtattacc
    18061 cacgcaacac cggtgttcgt cacggcaatg ctggtgctga ttggtgtcgg attctctatc
    18121 ggtaactatc tcggcggcaa actggccaga tcgttcagtt aacggcacgt tgaaaggctt
    18181 tttgttgctg ctgatggtga ttatgctggc aatcccgttc ctggcccgca atgagttcgg
    18241 cgcagctatt agcatggtgg tgtggggcgc agcaaccttt gcggtcgtac cgccgttaca
    18301 gatgcgcgtg atgcgtgtcg ccagtgaagc gccgggtctg tcttcatcag tcaatattgg
    18361 tgcctttaat cttggaaatg cgctgggagc agctgctggt ggtgcggtaa tttccgctgg
    18421 gctgggatac agctttgtgc cggtgatggg agcgattgtc gcgggactgg cattattgct
    18481 ggtgtttatg tcagccagaa aacaacctga aacagtttgc gttgctaaca gctaattaaa
    18541 caaaaacgca gaaggataac cttctgcgtt acagttgctg acaacgtgcg atttatcttg
    18601 ccggatgcgg tgttaacgac tcatccgcgt tgacatgaaa agacgcgtta ggcgtcgtat
    18661 catgcagcgc ttgtcggatg cggcgagtgc cttatccgac ctacatcgat gatccgcgta
    18721 ggcctgataa gcgtagcgct tcaggcaatg ctgcatttgc catcagttat tatgcagcga
    18781 gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac
    18841 gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag
    18901 tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat
    18961 cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa
    19021 actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc
    19081 cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga
    19141 cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt
    19201 taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatggt
    19261 gcttgccgta gtgatactcg atggtttccg cagaaatgtg cggtgccaga gcatctttag
    19321 catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat
    19381 acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag
    19441 atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc
    19501 attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt
    19561 tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg
    19621 acatgatcgg ctgtgccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagt
    19681 tcactacgtt cgattggcgc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga
    19741 atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg
    19801 gtacgcggtg agctgccacc ccaacgatat ggcttaccaa tttgctgcat cagtttattc
    19861 attgccactt tcgtagcttt ttgcaccttc gctttgtgcg cgtcggcaag agttcctgac
    19921 gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg
    19981 gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta
    20041 gaagcggtaa tggaagattt tttaacggaa gaagcggttt tgcttttggt cgtactttta
    20101 ctggcggctt ttttcgctgt tttctgggtc tttttggtgg ttgccgtgct tttcttttta
    20161 tctgcttttt tggtgatatg agtggtagca gaactctccc tggcttgctt tgaagcatgg
    20221 gccataggcg ttaaaggcag ggtggtaaaa agtaaagcac agagcgtgat cgaaatacgg
    20281 tttatccgcg ccactgagcg tccctctgtt aaggcaggtc tatcatgcct gcgtctgatt
    20341 tacaaaactt gatgattcta atgcatagaa gaagagaaag ttaatagctt ttattgttaa
    20401 aaaatacgtt tcgttggcga gcgcaaaaaa atgcagcagt gaaagctaaa aatggttata
    20461 aatacgccag tttgccggca tcagagaaga aacgaatatt tgaaatgcaa caaatagagc
    20521 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
    20581 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
    20641 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
    20701 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
    20761 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
    20821 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
    20881 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
    20941 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc
    21001 gaacgggcat ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa
    21061 atcccgcctc acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt
    21121 ctgttagcgt aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa
    21181 cctctggggc gagtaattcc tctttttcct gccagaccag catttttttg ccaccttgcg
    21241 ccagatagag taacaatttg ccgccagaaa cgaccaccag cgcacccgcc cgacgcgttg
    21301 gaaccagcgt tgccggatgt gcaggccagg gcagaagatt tccccacaca ttagccggat
    21361 cgttggcaga gagcgccact ggtgtatagt ggcgcgtttg cgtggcttgt gtcgccagat
    21421 cgcgcaatcg gtcaatagtc agacgttcag cgaattgcgc gccacccaga ccttctacaa
    21481 aacgacctcg cataattcgc ccggagtctt ccatacttcg acaaagcgtt tgcatcgatg
    21541 gaaacccgcc agggatattt tcggctatca ctgcctgacg actgatgatg ccgtagcggt
    21601 cgagcatatt ttccgccagc gccagcatcc tttcggtatc gtttagtggc tccacctgca
    21661 ataacgacca gcgtccagcc agatttggtg tgttgtaaga tacccgcggc gagacgggtt
    21721 gcgcatagac aggacgtcca cgacgagccc ggtgactgcg gcgagttgag gtgcgtgcgt
    21781 tggaactgct gcgggtgagg gcgcgtaacg gtgcccaaat gtcgctggtg atgacgcctt
    21841 gccagaccag cgcccataac gcctcttgca gggcagagag atcaaccgat tcgccgattt
    21901 tgtcgcgtat ccgttggctg atttgttgtg caaaccaggc tcctccgtca gccagaacag
    21961 cgactatcgc ttgctgcagc gccgaacgat tcgcctgatc cgcttcggcg ggagtgaacg
    22021 attctgcagc atattcctgt agatgcagtg ccaccaggcc gtcatcttca cccagttttt
    22081 tttgccccga ccagataacc gcaccggttg ccagtaattc atcgagcatt tctgacgaat
    22141 agtcgcgtac gcgggcaggc aggatctggc tttcccagag tgaggcgggt aaaccgactc
    22201 cggcaagctg ttcgatcacc cgcatcacgc catctacgcc ctcataaacg cctggcgatg
    22261 ttgaggcaaa gagcgccggg ctaccatcgg tggcgggtaa tacgccctga cgttccagca
    22321 gcaatcgcgc ataggtcgtg gctgcaacgg gacgcgtcgc ttctctggcg gcttgcagcg
    22381 agcgcaaacg cagacgacga aatacttcat cgctcaccca gatgtcttgt tgcagattca
    22441 tcaccagacc ctgttcacgc agttgctgaa gctgctcttc gacaatggca ataccgagac
    22501 taaactcatg agccagttgt tcagccgtga ccagagcatg agcccggaga tagcgcagaa
    22561 agaggtcgcg aagcgggtaa ctgactctat gtaaataaat ctctggcaat gactctggta
    22621 gtcgtacgcc gagggcatca cgcagcctgg cggcatcatc catacaggca agacgctcct
    22681 gtccgctaat catcgctggg aagattcgtt ttactgcaag aagattttcc agatacgacg
    22741 caacctcttc actgctgcct gtatgccgtt gcgccaggtc ttcaacggtc attggcccca
    22801 gttcgcgcag caggtcgaac aatccttctt cacctttcgc tcttctgcca ggagccagtc
    22861 gttgcaactc ttcttccacc tggcgaatga cctgcgggtc gagtaattcc cccggatcga
    22921 cctgtccgag tagattgcgc agtaactcgc tgtccagcga cagtacggat gcccggcgct
    22981 ctgccagcgg ggcgtcgctc tggtacataa attccgcgac atagccgaac aataaactgg
    23041 tggcgaaagg cgagggcgta gtagtcgtta catcggatat ttgaatttcg ccaccgttca
    23101 ggcgacgcat caaacgttcc agtgcgggaa gatcataaac atcttgcaga cattcgcgta
    23161 gggtttcgag aatgaccgga aaatccggat atccctgagc gatttccagc aactgactgg
    23221 cgcgcagccg ttgttgccaa agcggggtgc gatggcccgg cgtgcggccc ggcattaata
    23281 atgcccgcgc ggcgcattcg cgaaaacggg cggcgaaaag tgccgaactg cctaccgcct
    23341 cgcggacaat ttgcagcaac ttttctggtt caaacaaaaa aatcgcggca tcgggcaatt
    23401 taccatcggt gtcaggaata cgtgcaacaa tgccgtcatc actggcgacc accgacgcgt
    23461 cagcgcccca tagggcatgt attcgcccgg caatcgccag cgcccagggt tcatgcaccc
    23521 gtcttccata gggagagtgc aaaataatac gccagtcacc aatttcatca cggcaccgtt
    23581 cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgctgc tcctcaatca
    23641 acccctgaat attggcgatc gtattttctt ctgccagcca cgggggaata gtcccggaaa
    23701 agaacgcgcc atccgccagc aaatgaagaa aatcgccgat catctcgcct aattcagccg
    23761 gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc ggagcaggag
    23821 tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg ccgagagtga
    23881 taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagttcaccc acccgccgcg
    23941 aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga tccggaatgg
    24001 tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg ggcggtcaat atcccggtct
    24061 ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg
    24121 aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac ggtgcggcac
    24181 ggcgtacgcg ggagtaccat tcgtctacct gtaatgcatc catcgccgcg gcggcaacgg
    24241 tttgctgcgc aaggacgtcg agaggattat gcggtggtgt caggttttcc agcctgcctg
    24301 cgaacataca ctctacaatg actgcggaat cgactaaatc acgccgggta cgggggaaaa
    24361 acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt tgtaacccac
    24421 tggcaacaga aagcggcgtt gccacctgaa tcaccagatc caccgcgccc atatcaatcc
    24481 ccagttcaag actggaggtt gcgaccacgc agcgtaattc acccgatttc agcgcctgtt
    24541 cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca ataaaaacgt
    24601 cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa
    24661 cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca
    24721 gtttttccgc cagcccccgc gaattagtaa agacaatggt cgagcgatgg cgcaacactt
    24781 catcaaggat gcccgtttca atatatggcc agatggagcc ttcccggccg gcatggctgt
    24841 cttcgccagt gccgctggcg accgatgaga catcatccat attggcgacc ggtacgacaa
    24901 tccgtatctg cggatgacgc attgcgggcg ggttgactac cgtaaccggg cgatcgccac
    24961 caagaaatgc tgccacatcg ctggctgagc gcacagtggc agaaaggcca attcgctgtg
    25021 ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc gcaccacgtt
    25081 tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgacgccg cgtagcgttt
    25141 cgcgcgcgcg ggaggtcagc atcagataga gtgattcggg tgtggtgatc agaatatccg
    25201 gcggattacg ggtgagtttg ctgcgttcct gtgcaggcgt atcgccagta cggatcccta
    25261 cgcgaagatt gacttccgtt tcgccgcgcc gccgccgttc atcggcaata cccttcaacg
    25321 ggatctgcaa gttgcgctga acgtcggtgc ccagggcttt tatcggtgaa atatagagga
    25381 tgcgtgaggt tttacgctta tgcgcctcgc gggtatcttc gccgccttcg cggaagagcc
    25441 gatcgagggc gtagagaaat gctgccagcg ttttcccgga gccagtcggt gcaatcacca
    25501 gcgcatgttc gcctcgcgcc gccacatgcc aggtttgcga ctggacagcg gtcggctgtt
    25561 taaaggcgcg aagaaaccag tcgcgggtcg ccggtgaaaa cacgtccggc aggagcgatg
    25621 aagggtctgg attatctgcc attaacgcta ttccccgtga atatcatgca ggatgcgtgg
    25681 aatagcctca ctatagatgc ctgaatcatt atatgtcatc aggcatcgac tcgattacac
    25741 ctcttcggcg gtgggtagcg gccagcctcc cagacgtttc cagcggttga cgatttcaca
    25801 aaacagcaca gcagtgcgtt cggtgtcgta cagcgcggag tgcgcctggg tgctgtcgaa
    25861 gtccatgcca gcggtctggc aagcctttga caatacggtt tgtccgagtg ccagcccggc
    25921 cagtgcagca gtgtcaaaag tggcgaaagg gtggaacggg ttacgtttca gcgaggcgcg
    25981 ttctgcggcg gccatcataa agctgtgatc aaaattggcg ttgtgcgcca ccataatggc
    26041 gcggttacag ccgctcgctt taataccttt acgtacaact ttaaaaattt cgtgcagcgc
    26101 ctcgtattcg ctgaccgcgc cgcgatcggg atcgttcggg tcaatgccgt tgaaggcgag
    26161 ggcttctggt tgcaaatttg cgccgacaaa tggttcgacg tggaaatgta atgtggtgtc
    26221 cggcatcagc cagccttgtt catccatttt cagggtgatg gcggcaatct caagcagcgc
    26281 atcggttttg gcgttaaatc cggctgtttc aacatcgatc acaacaggat aaaaaccacg
    26341 aaaacggtcg cacagaccgg taagttgagc gttatcggac atctgattct cttaatacgg
    26401 ataaattgca gcgcgcatta tgacaaatat tacttgtcga tgcagtaaag atgcgggcgc
    26461 gatgagttca cgcccggcag gagattagtt gcccagaccg cgaccggcgt ctttttcttc
    26521 gattaactca attttgtaac cgtccggatc ttccacaaac gcgataaccg tagtaccgcc
    26581 ttttaccgga cccgcttcac gggtcacgtt acccccgttt tgacggattt tttcgcacgc
    26641 ttcagcggcg ttatctacgc taagcgcgat gtgaccataa gcagtgccga gttcgtattt
    26701 atccacgccc cagttgtagg tcagttcaat caccgcttct tcggtttccg ggccgtagcc
    26761 aacaaacgcc agtgagtatt tgtattccgg gttttcgctg gtacgcagca gtttcatgcc
    26821 cagcactttg gtataaaaat cgatggagcg ttgcaaatcg ccaacgcgca gcatggtatg
    26881 aagaagacgc atctttttat cctcaaaatg gctaacagat tcaaaatgtt gttttagtat
    26941 agcggcggct ttacgccgct cgcaatgttg gattacaacg tcgggtaatc ggtatagcct
    27001 tccgcgccgc caccgtagaa actttcggca cgctgtgggt taagctcagc tttgcgctgc
    27061 aagcgggcga ccagatccgg gttcgcaatc cagtcacgac caaatgccac cgcatcaatt
    27121 aaccctttgc cgatcagcgt ttcagctttt tctactgtgt atgcacctgc gccgataatc
    27181 ggaccgtgga aacgggcgcg tactttttcg cggaacgcat cagtatacgg ttcacccccc
    27241 gcccaatctg gttctgacat atgcagataa gcaatgccgc gtttacccag ttgttcaatc
    27301 agatacagtg catcggcttc ttcattcggg ccgttatccg tgttctggaa agtaccgatt
    27361 ggcgaaatgc gaatgccaat gcgatcggca ccccattctt caatctcggc atcgaccact
    27421 tccagtacca gacgtgcgcg attttccacg ctgccgccgt actgatcggt acgatggttt
    27481 gaagaaggag aaaggaactg atgcagcaaa taaccgtgag cagagtggag ctctaccaga
    27541 tcaaaaccgg cttcacgcgc gttagcaatg gcctgacgga aatcattgac gatacctgga
    27601 atctcttcca gttcaagcgc acgcggcatg gatgtttcaa cacggatcgc ctgaccattt
    27661 tcatcgcgca gagaagtacg tgttcccgcg ctaagtgctg aaggcgctac cggtgcctga
    27721 ccgccaggtt gcaggctggc gtgagaaatg cgtccggtgt gccacagctg cacggccata
    27781 tgaccatttt cagcatgaac gccagcggtg atttttttcc atgcggcaat ttgctccgga
    27841 ctatggatgc caggcgcacc tgcatatcct tttgcctggg cagaaatttg cgtggcttca
    27901 ctaataatca aaccggcact ggcacgttgg cgatagtatt ccgccatcaa cggggtagga
    27961 atgtcacccg gttcaatact gcgcagacgc gtcagcggtg ccataaaaat acggtttgcc
    28021 gccgtgatcg cgcccacttt cagtggggaa tacagttttt cagatgacat aaaggctccc
    28081 ggtcgtctgg tgataaaaaa tgcctgctaa acggcaggcg tcgcaataat gttttttaca
    28141 tgggccagcg cgttttccag tggctcgaaa ctgcgcgaaa ttttggcctg cagattcgcg
    28201 ccaagccaca gtgcgtaaag cacttgtgcc tgttgcagcg gttcgccaca aaaggttaaa
    28261 caatggttct cacggccatt ttccagcgcc tgagagagca gggcgatcac gccgcgagca
    28321 cctttatcca tcgcgctgcg catatcttct gacagatcgc acacttcggc agagagtttt
    28381 actgtcaggc aaccactgat ggttccatgt tggcaaaact ggttcagtgt ttgctggtaa
    28441 taagccagta tgcggtcgcg gtagttacct tcgccggatt gcagcaactc agtcagtcgc
    28501 tggtgatatg cggcgtaatg acgctcaagc atggcaacgc caaacgcttc tttagagcga
    28561 aagtagtgat agaaggaccc tttcggcact tcagcggttt ttagtaattc gcttagcccc
    28621 atcccggtga atccacgttg caggcaaagt tgctcgcccg tcgccaggag atgttcgcga
    28681 gtatcatgtt cggtgtgttt gttcatgcgt tggagtgtag tagaccagtc ggtctagtgc
    28741 aagcaaagtt gctactttgg acattaacag cttcaataat ggtaataatc ttatctgagg
    28801 agggcgaagt ggcggagcaa ttagagttct ttcccgtcca gagcccgtgc cggggaattt
    28861 gccagtctga cgaacgcggg ttttgtcgcg gctgttttcg tagtcgtgat gagcgtttta
    28921 actggaataa aatgagcgat ggcgaaaaac aagaggtgct gcgcctttgt cggcagcgat
    28981 taatgcgcaa attacgcgca gataagccag cgtcatcaga cgaaccggag caaccatcac
    29041 tcttttgaac ctaatcttgc gtatactcct ctcatatctg ataagaggaa gcgattatgg
    29101 ttcagcgtat tactattgcg ccgcaaggcc cggagttttc ccgttttgtg atgggctact
    29161 ggcgattgat ggactggaat atgtccgccc gccagctggt cagttttatt gaagagcatc
    29221 tggatctcgg cgtgaccacc gtggaccatg ctgatattta tggtggctat cagtgcgaag
    29281 cggcgtttgg cgaggcactg aaactggcac ctcacctgcg tgaacggatg gaaatcgtca
    29341 gtaaatgcgg tatcgcgacg accgcgcgtg aagaaaacgt cattggtcat tacatcactg
    29401 accgcgatca catcattaag agcaccgaac agtcgctaat taatctcgcg accgatcatc
    29461 tggatctgct gttaatccac cgaccagacc cgttaatgga tgccgatgaa gtggcggacg
    29521 cgttcaaaca tctgcatcag agcggcaaag tgcgtcattt tggcgtatcg aactttacgc
    29581 ctgcgcaatt tgccctgttg caatcacgtc tgccgtttac ccttgccact aatcaggtgg
    29641 aaatatcccc ggtgcatcag ccgttactgc tggatggcac gctcgaccaa ctacaacaac
    29701 tgcgtgttcg tccgatggcg tggtcctgcc ttggtggtgg tcgtctgttt aatgatgatt
    29761 atttccagcc gctgcgtgat gaactggctg tggtggcaga ggagttaaac gcgggctcga
    29821 ttgaacaggt ggtttacgcc tgggtattac gtttaccatc gcagccgctg ccaattatcg
    29881 gttcaggtaa aattgagcgc gtacgggcag ctgtcgaagc agaaacactg aaaatgaccc
    29941 gtcaacaatg gtttcgtatc cgtaaagcgg cactggggta cgacgtaccg taagcacttt
    30001 taggaatagc cgccgttcaa aaatgtgtca ctggtttaca cttattcagg aatgcacaat
    30061 gaacggaggt cctatgaaac gttttagtct ggctattctg gcgctggttg ttgcaaccgg
    30121 cgcacaagct gccagtgaaa aagtcgagat gaacctcgtc acgtcgcaag gggtagggca
    30181 gtcaattggt agcgtcacca ttactgaaac cgataaaggt ctggagtttt cgcccgatct
    30241 gaaagcatta ccccccggtg aacatggctt ccatattcat gccaaaggaa gctgccagcc
    30301 agccaccaaa gatggcaaag ccagcgccgc ggaatccgca ggcgggcatc ttgatccaca
    30361 aaataccggt aaacatgaag ggccagaagg tgccgggcat ttaggcgatc tgcctgcact
    30421 ggtcgtcaat aatgacggca aagctaccga tgccgtcatc gcgcctcgtc tgaaatcact
    30481 ggatgaaatc aaagacaaag cgctgatggt ccacgttggc ggcgataata tgtccgatca
    30541 acctaaaccg ctgggcggtg gcggtgaacg ctatgcctgt ggtgtaatta agtaattaag
    30601 aggccagcgt accttgcggt ggtgcttgct caagttgcga aagcgagcag tacagccgcc
    30661 agacaattgc cgccagctca cgggcggcag gttgatgatg acgggcaagg ctgtcgcaaa
    30721 tccgctgtaa ttcttcaagt gtggccgcca gtgatttttg ctgaacgcca cgctcactca
    30781 tcactccccg caacagtgaa atacagttat cccgtactcg cgataacgga tcggaacgcg
    30841 attcccagtc gcgcaattgc cagacaacat gagaacagtt cagcagcact acaccccagc
    30901 gtaataacca acggcgagcc agcgcatcct ggctgttact caactgactg acgtgatgat
    30961 aagtgagcga ttcaaattcg ctttcactca gtgttggatg gcggcttagc tgatcgacaa
    31021 aatcccggcg cagcgcgcga atatggcggc ggcttttacg agcatccgat cctggacgca
    31081 gaatggcgaa cgctaaccac gccaacgcga cgccaacgat ttttgccaga ttatcgttaa
    31141 gaaaatcagc aaaatcatac accgggggat tagtgacagc gataaaagaa cccataaaaa
    31201 caatcagttg cccccacaat gcggcaaatt ttggcatctg caatttaaga agctgcattg
    31261 tcgccagcag tggaaagaga aacagtaaaa attgccacag atcgctaatc tggaccatca
    31321 gaccaaattt gaccacaaag ctgaatagcg aaagtaacac cagcgtgcgc atcagaagtg
    31381 acaacgactt aaacggtgct gcgacggcgg agtagagtac gcagctaatt gctgccagcg
    31441 ttaatgcatt ggcaccggca tcccattgcg aagcaatact ccatgcgcca atcatcatca
    31501 acgtacaaaa tgtacgcagc ccgctccaca tagcttcggc gttatcggta tgacgagcca
    31561 ggccggatgt ccgtgggagt ctggtatgat catctacacc gctttgcaga cgatgtaatt
    31621 cctgactact ttgcagataa aggcggcaaa aataacgtag tcgctgccag aaggcgacgt
    31681 gccgatagtc ggcgacgttg gtcgggcgta gcggggcgat aatacgtgcg acggtgtaaa
    31741 catctgtttg cgaactggcg agcgccgtca gcaactgttc gagaatttct cgtgtggcac
    31801 ctggcggtga gggccagttg agcaacatac gtcgcaggct agagatgaca ctggtcatac
    31861 gtaattgctg gtggagcagc gcattaaggc gcgcgttttg ctggcgaaaa cgatagtggc
    31921 tccagaaagc ctggatacgc agcaaattca tggtcagtat ctgcccaatc accccttcat
    31981 gtgctgcacg aatggcatcg gttgtttcag gctgccagag taaactggca tgttcaagta
    32041 gtcgggcgtg catgtttttc aatgcggtta aaagggctgt agcatcggaa ctgctaggca
    32101 ggatcatcat catcatgccg ccgcacaaaa tgccgacaat cacctcgcaa acgcgcgcct
    32161 gagcgatatc ccacagctgg ctggcctcag taatattaac catcggaaag gcgatgatgg
    32221 cagccgtgta gcccgccagt tgaaatgcat acgcgacgtt attcgtgaag tgcgcacagg
    32281 cccaggtaca aaagccaagc cacgccgaca tgctcaatag aaaaaaccac ggctcattga
    32341 gcgtatgccc ggcaagaagc agtgccgcaa tggctccgag caaactgcca gcgatgcgtc
    32401 cgaggctttt gctgataaca ccgccaacgg tgggaaagct aaccactgca gccgaggtca
    32461 tcgcccaata gggttcatcc agatttaaat aataggcaac cgtcagcgcc agacacatgg
    32521 caatggtatt gcgtaacgca taacgccatt gcgccagcgt ggccctgaac cagggcaaat
    32581 tgcgcaagga ccatgacgat gcgttcatta ttgacctacg gcaatgctac aggtggtgcc
    32641 cgacaccaac gtgacatcgc ctgggacttt atccagcgca aagcggacgg gaacgcgttg
    32701 ggcgagtcga acccagggga cgttaggttt aacatccgga ataaggcttg agtcactttc
    32761 aacgctctga tcataaatcg cccgaccgat actggaaacg tgaccctgta acgttttgtt
    32821 atcactatag agggtaattt gtgctggtgc accttcgcgg atatggcgca acttggtttc
    32881 ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca agggcaaaca gcggtttccc
    32941 ggtgtcagcg taatcgccga tgcgggtagt gaggttagtc acccaaccac tcaccggggc
    33001 gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc agcgtggcct gtgcggcatt
    33061 aacactggcc tgcatcgctt taacattgag gttagcggta tcgagttctt cggcagaaat
    33121 aaaattttgt gagagatgac gacgacgatt cgcttcgtta ttggcttttg cgaggtcaga
    33181 ttgcgctttc gccagctgag cctgtgcgtt cagctcggcg atctgaaagg gcgttttatc
    33241 gatcgtgagc agaagatccc ctgctttaac cagctggttg tcttttatat tcagctcaac
    33301 aatacgtccc gacacctgtg gggtgataga aacctgttct gcgcgtattt tcccgtcgcg
    33361 tgtccacgga gattgcatgt aataattcca cagccaccat ccggcaagaa ccgcaactac
    33421 cgctacaatg attgttgaga aatacttaat tgttttaatt gacatagtta ccacgcaatc
    33481 agtattgcca gagcaaggca aacgcaaatc gcaaacagcg ataaatccat taacaaggga
    33541 tgccagatgt caccggcgta gatccagcca cgaagcaggc ggtgtacgac aagccagatg
    33601 acgaatccaa acgcgaaggc tttgaaaaac ggaggaaagt agacggacgc accgaacacc
    33661 agatcttgta agggcaatcc tgttgcattg agcataaact tcacaaagta gattccttta
    33721 cgaccgaata tattgcgtta gattaataaa tattctttaa gtgcgaaaaa tttacgcgca
    33781 atttctgaaa aatatgtctg acggtaacca aatgcagcaa tacatttgtt ttagcaatac
    33841 aattgctgca cactattcta aaagccgcat aatatcttag caagctaatt ataaggagat
    33901 gaaattggaa tcgccactag gttctgatct ggcacggttg gtgcgcatat ggcgtgctct
    33961 gatagaccat cgcctgaaac cgctggagtt aacacaaacc cattgggtta cgttacacaa
    34021 tatccatcag ttacctccag accagtcgca aattcaactg gcaaaagcga ttggcatcga
    34081 gcagccatca ctggtccgta ctctggacca actggaagaa aaagggttaa tttcgcgtca
    34141 aacttgtgcc agcgatcgtc gggctaaacg tattaaactg acggaaaagg cagagccact
    34201 gatcagcgaa atggaagctg ttattaacaa aacccgcgcg gaaatattac atggcatctc
    34261 cgcagaggaa ctggagcaac tgattacgct catcgcaaaa cttgagcata atatcattga
    34321 gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca gtaacctgac cacacgcaaa
    34381 cttatttagc gcggagaaac ggtcacctga ctgccattgc tggccagtac gacacgttgg
    34441 cccggagaga aacgagtgtt gccttgtttc tgtaccacca tgatggtatt accatcgtct
    34501 ttacgaattt ccagctcgac accctgcgtt ttgttcattg cactctgtac gccctgacca
    34561 gctacgccac ctgcaacagc gcctgctgca gtagccagag aacgcccggt tccgccacca
    34621 acagtattcc ccaggaaacc accaagaaca gcaccgccaa ttgcaccgat aacgttggaa
    34681 tcatcaccgc cctgaatctg taccggacgt acgttaacga tggtgccata gctgacattc
    34741 tgtacttgtt tcgcttcaga agcggtataa acatcccctg acagggtgtc gttattaaca
    34801 caaccgacaa gagacagacc taccattgaa acaaccaata cgcgtttaat cattgaaaaa
    34861 tctcctgttc accatgaaac gccacgcaag catccctcat ggtcaaagta tatggcatat
    34921 gtgaatccat ggttacatgt tctaccaaat atgagcaaat cataatgaaa gtaggatcaa
    34981 ccaattataa acaaaacatt catgatgatt attgatttga agataatgtt ttactaccaa
    35041 cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt ttatgtttga gtggtgcgca
    35101 aacgtcagat tgaaaaagga tgaactatga aatcgggccg ctttattggc gtaatgtcag
    35161 gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat agatgaacac cgggtcgcac
    35221 agctggcaag cttgagctgg ccgatcccgg tgtctctgaa acaggctgta ctggatattt
    35281 gccagggcca gcagcttaca ctttcgcagt ttggacagct tgatactcaa ctcgggcgac
    35341 tttttgctga tgcggtcaat gccttgctta aggaacaaaa cctgcaggcg agagatatag
    35401 ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc gacgggcgtg gcaccacaca
    35461 ctttacagat tggcgataac aatcaaattg tggcacgcac cggaattacg gttgtcggtg
    35521 attttcgccg tcgcgatatt gccttgggag gacaaggcgc accgctggta cctgcgttcc
    35581 atcatgcctt gctggctcac tcaaccgagc gacgaatggt gctcaatatt ggcggcatcg
    35641 ccaatctgtc actgctcatt cctgggcagc cggttggcgg ctacgatacc ggtcctggta
    35701 acatgctgat ggatgcctgg atctggcgtc aggccggtaa accttacgat aaagatgccg
    35761 agtgggcacg ggcgggtaaa gttattctcc cactgctgca aaatatgctc agcgacccat
    35821 atttctcgca acctgcaccg aaaagcaccg gacgcgaata ctttaactac ggctggctgg
    35881 agcgccattt acgccatttt ccgggtgttg atccccgtga tgttcaggcc acactggcag
    35941 aactcaccgc cgtgaccatt tctgaacaag ttttgttgag cggtggctgc gaacgattga
    36001 tggtatgtgg tggaggtagt cgtaatccgc tactcatggc gcgtctggcg gcattactgc
    36061 caggcacaga agtcaccacc accgatgccg ttggcattag tggcgatgac atggaagcat
    36121 tggctttcgc ctggcttgcc tggcggacgc tggcgggatt accaggaaat ctgccttccg
    36181 tcactggcgc aagccaggag acggtactgg gggctatttt ccctgctaac ccgtgatatc
    36241 agagttaact gaatttttct taccgcgtct gccgttacac ttggaacgtt agggagggcg
    36301 tattgccctc cagaccagga aagtcttcgg gaaatgtcta tgaaaaaact gttactgatc
    36361 tgtttgccgg tgcttctcac cggctgtagc gcgttcaatc aactggttga gcacatgcaa
    36421 accgctacgc tggaatacca gtgtgatgaa aaaccgttga cggtcaaact gaataatccg
    36481 cgccaggaag tcagttttgt ttacgataat caactactgc atctcaaaca gggcatttca
    36541 gcgtctggtg cacgttacac tgacggaatc tatgttttct ggtcgaaagg cgatgaagcg
    36601 actgtctata aacgcgaccg catcgtattg aataactgtc agttacaaaa tccacagcgt
    36661 tgagattttt ccaggggcgg cgcacaatag cgtcacccac tgacaatccg taaagaaaac
    36721 catgtctgat aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat acaccaaagg
    36781 cgggttacgc cgtcgcgatc ttcccgccga tccattaacc ctttttgaac gttggctctc
    36841 tcaggcgtgt gaagccaaac tggcagaccc taccgcgatg gtggtcgcta ccgtggatga
    36901 acatgctcag ccttatcaac gcatcgtttt actcaaacat tacgacgaaa aaggcatggt
    36961 gttttacacc aacctcggta gccgtaaagc acatcaaatt gaaaataatc cgcgcgttag
    37021 cctgctgttc ccgtggcata cccttgaacg ccaggtgatg gtgatcggta aagcagaacg
    37081 actttcgact ctcgaagtga tgaaatattt tcatagccgc ccgcgtgata gccagattgg
    37141 tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg aaagtaaatt
    37201 cctggagctg aagcagaagt ttcaacaggg cgaagtgcca ttgccgagct tttggggcgg
    37261 ttttcgcgtc agccttgaac agattgagtt ctggcagggc ggtgagcatc gcctgcatga
    37321 ccgctttttg taccagcgag aaaatgatgc gtggaagatt gatcgtcttg caccctgaaa
    37381 agatgcaaaa atcttgcttt aatcgctggt actcctgatt ctggcacttt attctatgtc
    37441 tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat atacatggag
    37501 attttgatgg caagcagtaa cttgattaaa caattgcaag agcgggggct ggtagcccag
    37561 gtgacggacg aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc gctctattgc
    37621 ggcttcgatc ctaccgctga cagcttgcat ttggggcatc ttgttccatt gttatgcctg
    37681 aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg taggcggcgc gacgggtctg
    37741 attggcgacc cgagcttcaa agctgccgag cgtaagctga acaccgaaga aactgttcag
    37801 gagtgggtgg acaaaatccg taagcaggtt gccccgttcc tcgatttcga ctgtggagaa
    37861 aactctgcta tcgcggcgaa caactatgac tggttcggca atatgaatgt gctgaccttc
    37921 ctgcgcgata ttggcaaaca cttctccgtt aaccagatga tcaacaaaga agcggttaag
    37981 cagcgtctca accgtgaaga tcaggggatt tcgttcactg agttttccta caacctgttg
    38041 cagggttatg acttcgcctg tctgaacaaa cagtacggtg tggtgctgca aattggtggt
    38101 tctgaccagt ggggtaacat cacttctggt atcgacctga cccgtcgtct gcatcagaat
    38161 caggtgtttg gcctgaccgt tccgctgatc actaaagcag atggcaccaa atttggtaaa
    38221 actgaaggcg gcgcagtctg gttggatccg aagaaaacca gcccgtacaa attctaccag
    38281 ttctggatca acactgcgga tgccgacgtt taccgcttcc tgaagttctt cacctttatg
    38341 agcattgaag agatcaacgc cctggaagaa gaagataaaa acagcggtaa agcaccgcgc
    38401 gcccagtatg tactggcgga gcaggtgact cgtctggttc acggtgaaga aggtttacag
    38461 gcggcaaaac gtattaccga atgcctgttc agcggttctt tgagtgcgct gagtgaagcg
    38521 gacttcgaac agctggcgca ggacggcgta ccgatggttg agatggaaaa gggcgcagac
    38581 ctgatgcagg cgctggtcga ttctgaactg caaccttccc gtggtcaggc gcgtaaaact
    38641 atcgcctcca atgccatcac cattaacggt gaaaaacagt ccgatcctga atacttcttt
    38701 aaagaagaag atcgtctgtt tggtcgtttt accttactgc gtcgcggcaa aaagaattac
    38761 tgtctgattt gctggaaata atgcattaag tggaaagggg gagtgagaaa tcactccccc
    38821 tggtttttat acagggaaca tgatgaaaaa tattctcgct atccagtctc acgttgttta
    38881 tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc cgcctgggcg cgaacgtctg
    38941 gccgctgaac accgttcaat tttctaatca cacccaatac ggcaaatgga ctggctgcgt
    39001 gatgccgccc agccatttaa ccgaaattgt gcaaggcatt gccgccattg ataaattaca
    39061 cacctgtgat gccgtattaa gtggctatct gggatcggcg gagcagggtg aacatatcct
    39121 cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg aaatattttt gcgatccggt
    39181 aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt gtcgcagagt ttcatgtgcg
    39241 gcacggtttg cctgccagcg atatcattgc gccaaatctg gttgagctgg aaatactctg
    39301 tgagcatgcg gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac tcattgcgca
    39361 agggccacaa attgtgttgg ttaaacacct ggcgcgagct ggctacagcc gtgaccgttt
    39421 tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc agccgtccgc tggtggattt
    39481 tggtatgcgc cagccggtag gtgttggtga tgtgacgagc ggtttactgc tggtgaaact
    39541 gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg accgctgcag tctacgaaat
    39601 catggtgacc accaaagcaa tgcaggaata tgagctgcaa gtggtggctg ctcaggatcg
    39661 tattgccaaa ccagaacatt acttcagcgc aacaaagctc tgaaatatct catttcggcc
    39721 cacaacgacc tgtgggccga tggctattac tttaagcctt ccgctgacag cgcgtcttgt
    39781 acttccggac gttcagccat acgttgcata aatgctgcaa tgtgctcaaa cccttccaga
    39841 ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca gataggcatc agcaattgta
    39901 aatctttgcc cgcagatcca gtgctcatcc ttcagtgcct cgttcacata ttgcagcttc
    39961 ttctccagct gcgcgcgaac tgtcggtttg tactcttccg gtgtatcagg gcgaaacaga
    40021 ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca gccattcgat ggttttatag
    40081 cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc ggcaagatac
    40141 tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat ccagcagcaa tgcaggcacc
    40201 tgccccttag ggttaacggc aaagtaatcg tcaccgtttt cgagacgttt tttcattaaa
    40261 tccacactga cgagggtaaa atcctttccg ctctcacgca gggtgatatg ggaagcgaga
    40321 gagcaggcac ccggtatgta gaacaatttc atcggtaact ccttttgtct gtggtttaaa
    40381 gtatggtagt gcgttgctga acaaaaaaaa agccgctaac gaaaagttag cggcttgtaa
    40441 aagagtttcc ctgaatttta cgctacggct gctttcgccg ctttgtctgc agcgtcatcc
    40501 tgcgtcatgc ggtgcagttt cggcgcggtc agcagcatca gtactgcaat aacggcagta
    40561 gcgacaccaa tctgcaagaa tacgcgacca tagacttcca gtgacatcag cggatcggta
    40621 acgttatccg gcacagccat catacccgca acataaccac caatcaggtt tgcaccggca
    40681 gtggtcagga accagctacc cataatgaag cccatcagac gctgcggaac gagttgagca
    40741 accattgcca gacccagacc agagatcatc agttccccga tgctctgcag gccatagctt
    40801 gcgaccagcc agcttacaga cacgatacca gcgtcagacg cgaatttcgc acccagcggc
    40861 agaatcagga acgcaccaga acacatcacc atgccgattg caaacttggt tggcatcggc
    40921 agggtatcgc ccatcttgtt atagatagcg gccagaatcg gactaccgat gatgatccag
    40981 aacgggttca gtgcctgata ctgctcaggt tctacggcca gacccagaat ggagtgctca
    41041 acgttacgaa tcgcaaagaa gttcagtgac gttggcatct ggctgtacag cacgaagaag
    41101 ataatggctt cgagcatcag gatgaaggca acgatcattt tacgacgcgc agcacctttc
    41161 atcgcgaagg cttctttacc gaagataacc acgataccga aggcaacaac gcccagcgcc
    41221 atacgcgcaa cttcctgatt gtgcagcagc caggtggcga tagcgatcag tgccacaaca
    41281 ccaataatgg tcagcagcag gttacggtag ttgataggct cgaagtctgg ttttgaaccg
    41341 tactgtttaa cccagcgttg gcagaaggcg aagttaacga tagtgatcag caggcctaca
    41401 acgctcaacg caaacgcaac actccagccg tatttcgcgg ccagccacgg cgtagcaatc
    41461 atagagaaga aagagccgat gttgacggac atgtagtaca tggtgaatgc accgtccaga
    41521 cgcgggtcgt ttttctcata gcatgtagaa agcagagaag acgggttagc tttaaacagg
    41581 ccgttaccga ccgcaatagc cgccataccc atataaacga taccggcgtc gtgaccagac
    41641 caggcaacca gagcataacc aatcgccagc acaatagcgc cgagcataat tacgcgttta
    41701 gtacccagta ccttgtcacc taaccagccg ccgatagcga ccagaccata aaccagggca
    41761 ctaaaggaag agaaaagggt gattgaatcc gcttcagaca tacccagttg tttaaccagg
    41821 taaacagcca taattccttg taggccgtaa taaccaaaac gttcccataa ctcaatcgag
    41881 aagatgagat agaacgcctt cggttgtttg aaagcgttca aactgacgct ttcagttggt
    41941 ttttggtttg cagtggacac ttttacctct ttttttacat cccatattaa cgggggtgct
    42001 ttctcgcgtt atgtataaaa aacatacgcg caattgttat agtgggaggg gaaacggcag
    42061 gtaatgttca ctatcttgca ctttcagaca agtcctttgt aataatctgt tacatataac
    42121 tgaggcgggg aattcaacca ttccgggaaa tgttatccag cgttaaattt ttcaaaaaac
    42181 gtaacggcag attttttcac tgcactgaga ggggggtgat ggcttaacgg cgatatgttc
    42241 tgctatttag cgtttttact agtgagatag tccagtttct gaaaaatagc cagtgtaatg
    42301 ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg attttaacct gattcttgca
    42361 atgtttttac attgcgacag catcgtgatc ttgatcacga atgaatagaa aatttcgcta
    42421 ttaaaaaaac aattaacctg catttttggt tatccgcaaa cggattacct gatgataacg
    42481 ctaagcaatg gcatcattga cgttaaaaag atgttttaaa agaggataaa gaaaggttat
    42541 caatggggta atcggtgtta ccccttttct tcagatgtca actttctctt tgtattcaca
    42601 aagatcttca ataatacaag agccacagcg gggcttgcgg gcaatgcagg tataacgccc
    42661 gtgcaggatc aaccaatggt ggcagtcgac tttaaactct gccggaacca ctttcagtag
    42721 cttttcttct acctgttcga cgtttttccc cggcgcaaat tgagtacgat tacaaacgcg
    42781 gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat gcagtgttta atacgacgtt
    42841 ggctgtttta cgacctacgc cgggcagggc ttcaagcgca gcacgatctt ccggaacctc
    42901 gccattatgc tgctccagca agatacggca ggttttgatg atattttctg ctttgctgtt
    42961 ataaagccca atcgttttga tataggtttt caccccttca acgcccagtt caagcatcgc
    43021 tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac tgacatcggt
    43081 cgcctgagcg gaaagcagta cggcaatcag caattcaaaa ggcgaactga aattaagctc
    43141 ggtggtggga tgaggattgt tctcacgcag gcgagtgagg atctccaggc gttttgcttt
    43201 gttcatcaga cattccctgt ttcaccgttt ggcaatgcac gttcggcagc tgcttcagca
    43261 cggcgctttt tcattctttc atcaatcagg tattttcctg ccagcatcag tcccaggcca
    43321 ataaatgcac ctggaggcag catcgccagc aggaaagggg agtcggtgtg gaaaatttcc
    43381 acgcgtaata cttttgccca gctacctaac agcgcatctg caccgtcaaa caatgtgcca
    43441 ttgccgataa tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc gcccatacca
    43501 attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa ggcttcagcg
    43561 cggcccacaa cgatacagtt agtgacaatc agcggaataa aaatccctaa tgattgatat
    43621 aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga ggcgatgatc
    43681 atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt cgaaatggtc
    43741 aggttggtca gcgtcagtac cagcgtagtc gcaagtccta aacccagagc gttagtggca
    43801 gtggacgtga ccgccaacag aggacaaagg ccgagcaact ggaccagagc agagttgttt
    43861 ttccacaacc cctgaacaat aacgtcttta atttcgctca cgctttattc tccacaggca
    43921 ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca atccggcgcg ttttaccgca
    43981 ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatc accaccatct
    44041 ttcttcaccg cccagtgcgc atcatccgca ccactgattt ttttacccgc aaaatgggtg
    44101 atccagtcag aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc gtggtgctct
    44161 gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc ccaccagcag ctgaatcgca
    44221 ccggaatagc catctggcgc ggttgcttcc agaacggcgg ctaccggttt gtcatcctgt
    44281 ttggcgatgt aaacccgatg ctcaccttta cctaactctg gcgcagttac cagatagcaa
    44341 ctctgtgcca gcgcattgtt atagcgttcg gctggcagca cctgatcaaa taatgccttt
    44401 tgttgcagac tggcctgttc agcaatcgtc gttttggtca tctggttgat ggccgcagtt
    44461 aaccctgttg aacccgctgc aaatagcgct aacgtaatgc cgtgttttcg gatagttttc
    44521 agcatggttt accctttgcg atggccgtag acgcgcggac gcgtgtagta atcgatcaga
    44581 ggaaccgtga tgttcgccag caggacggca aaagccacgc cgtcaggata gccgccgaaa
    44641 ctgcggatca accagactaa taagcccgcc agcgcgccga aaataagacg accacgattg
    44701 gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat ggtcgcacca
    44761 gacagcagat gaatttgcgg tgctgccagt gtttctggtg agaacaacca gcccaacgtt
    44821 gcgcataacg ccagcgttac taagaagctg aggggaatat gccagcgaat cgctttctgc
    44881 catagcaacc acaggccgcc agccagccag gcgagattta cccattgcca accagcgccc
    44941 gccagaatac cgctgtagat cggatattgc ataatctgtt caaccgaatg accggcacgg
    45001 acagaggttt taaatgtatc cagcggtgtc gcctgactaa tgccatcaat acctaagcgt
    45061 agtgtgttca tatcaccacc actggcggta tgtccgctga aaataacctg tatggcgtcg
    45121 ataaaaccag ggatgttgac cgcaatttca tgtggcggta accagctggt catctgcacc
    45181 gggaaggaga tcagtaagac cacataacca atcattgccg gattaaacgg gttttgcccc
    45241 agaccgccat acaactgttt agcgatgatc accgcaaaca ccgtacccag cacgaccatc
    45301 caccatggcg cgaggggggg aatacttacc gccagcaata agcctgtcag caatgctgag
    45361 ttatctttca acgttgcggc taccgactgc ttgcgtagtt tgagtacgag agcttcggct
    45421 aacagagcac taaccgatgc caacaggatc tgaacgagag taccccaacc aaaaaaccac
    45481 agttgcgctg cgattcctgg cacggctgcg agcaacacca acagcataat gcgcgatgtc
    45541 tggcgctggt tatgggtata aggggagcta gctattctga ataccattta gtcctcgttt
    45601 acaaccttct gctgggcggc ttttttggcc tgagcgcggg caatagccgc ggcaactgcc
    45661 gctttgcgcg gatcaacctg ttcttctggt tctgcgttag tctgttgctg ttccagcttg
    45721 cgcgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc aacctgttct
    45781 tctggttccg cattagcctg ttgctgttcc agtttgcgcg ctttggctcg ggcaatagcg
    45841 gcttcgacgg cggctttgcg cggatcaacc tgttcttctg gttccgcatt agactgttgc
    45901 tgttccagct tgcgcgcttt ggcacgggca atagcggctt cgacggcggc tttgcgcgga
    45961 tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg cgctttggcg
    46021 cgggcgatag ctgcttcaac ggcagtttta cgtggatcag caacggttgc tgcgtcgtta
    46081 gtttgctgca gttctgcctg tttcgctctg gcttgcgctt tacgggcttc ccgtgctgca
    46141 ataattgcac tgttatccgg gcgttcgccc gctttaatca caataggctg tgtagcctgg
    46201 gcctgtttct ctttcacccg cgccagagcg gcagcaatcg catctttatc tttggctgca
    46261 ggttgaacgg ctgcgctctt atgtcgttca aggcgagccg ctttttcgcg ctccagacga
    46321 gcctggcgcg cttcgaaacg cgctttggct tctgcggcgc gcttttcttc ctgacgaata
    46381 gccgcaattt cagctttttc ctgacggaaa tattgcacca ggggaatatt gctcgggcaa
    46441 acccacgcgc aagccccaca ttcaatgcaa tcagcaatgt tatgcgtggt agctttatcg
    46501 tgttgctgac ctttgctgaa ccagtacaac tgttgcggca aaagatccgc agggcaggcg
    46561 tcagcacagg cgctacaccg gatgcagctt tgttcttcct gtggttcgcc aagttcattg
    46621 gcagagggag ccaacagaca gttggtaatc tttacgaccg ggacatccag ccatggcaag
    46681 gtaaagccca ttagcgggcc acccataatc accatttgat cggcagaggg gcagaatccg
    46741 gcatcattca ataaatgacg cactggcgtc cccagccgtg cccagacgtt gcccgggcga
    46801 gcgattgctt cgccagtcag ggttacaaca cgctcggtaa tcggctcgcc atcaataacg
    46861 gcacgtttca ctgcataagc agtgccgacg ttttgcatta atacgccgat atcggatgaa
    46921 cgcccgccat gtggaacctg cttcccggtc agaatgtagg ttaattgttt agcaccgcca
    46981 gaaggatatt tggttggaat cacccgcaga gaaatatcgt tagagtccgc cagcaccgcg
    47041 cgcagcatgg aaatcgcctg cggtttgtta tcttcaatgc cgataagaat ttcgcgtggc
    47101 tgcagaatat gcgcaagaat gcgaataccc tctacgacct gagccgcgca atcctgcatc
    47161 aaacggtcat cggcggtaat gtacggctcg cactcagccg cgttgataat caacgtttca
    47221 atcttatctc cgccaccctg caatttaacg cctgtcggga atcctgcacc gcccagcccg
    47281 gctacaccaa actgatgtat gcgctcgatt aactcttcgc gactgcgagt gcgataatcg
    47341 gcccagccgt cgcgcgggat ccagcagtct tcaccatcgg catcaataat cacgcttaat
    47401 tcagctaaag ctgaaggatg agccgtagag tggggcgcaa tagccgtaac ggtacccgag
    47461 gtgggcgcgt gaacaggcag cattttgccg cgaccacggg taagcggctg gccgcgcaat
    47521 actttatcgc cgacgctaac gcacaactca ccttcagcgc caatatgctg tttcagtgga
    47581 ataacaaaac gctgcgccag tggtacctgg cgcagtggtg taccgttgga ctgggttttc
    47641 atctccggtg gatggatgcc gccgttgaaa tcccagattt tattttttct gaatgcagag
    47701 aataacttaa gcatggtgtt ccacgggaat gatacgcacg ggaatggtgt tcagatccca
    47761 tttccaggag tcaggtgttt ctgcgaccgg ttgcaacgag atgcagtgcg tcgggcacgg
    47821 atcaacacat aaattgcagc ccgtacagag atcactcatt accgtatgca tggcacgggt
    47881 agcgccaacg atggcgtcta ccggacacgc ctgaatacat ttagtgcagc caatacagtt
    47941 attttcatca ataaccgcca ccatccgcgc aggcgttagc tcttgcgctt cgccatccag
    48001 cggctgcggc tcgacattaa gcaactcggc aatttttagc atcacagctt cgccacctgg
    48061 ggcgcaacgg ttgatttttt caccgttaca gctgatggct tccgcgtagg ggcgacagcc
    48121 gggataaccg cactgaccac actggctctg cggtaagatt tcgtcaattt tctcaacgac
    48181 cggatcgtct tccaccgcaa aacggcggga ggcataaccc agaatggcgc caaacgccag
    48241 gcccagcagg ctcacggcgg caacggcaat ccagatagca ttcattacaa cttcaccaaa
    48301 ccactaaagc ccataaaggc cagagacata agacctgcgg taattaacgc aatggcatta
    48361 ccgcgaaaag gtgccgggac atcagccaca gcaaggcgtt cgcggatggc tgcgaagaga
    48421 accatcacca gcgaaaaacc gacagcggcg gaaaaaccgt acagcgccga ctgcaagaaa
    48481 ttgtgcccga gattgatatt cagcaacgcc acgccgagca cggcacagtt ggtggtgata
    48541 agcggcaaaa aaatccccag caaacggtaa agcaccgggc tggttttgcg caccaccatc
    48601 tcggtgaact gcacgaccac agcaatcacc agaataaatg ccagggtgcg caagtaaatc
    48661 agattaagtg ggatcaaaat ccacgtatcg ataagccagg cgcaaataga cgccagcgtc
    48721 atcacaaacg ttgttgccag ccccatgccc atcgcggttt ccagtttttt ggaaaccccc
    48781 ataaacggac agagaccgag aaacttgacc agtacaaagt tattgaccag tacagttccg
    48841 acaaacagta acaggtagtc agtcattttt cggcctgaaa taaaaaagcc gcctattatc
    48901 cgttaatcca gagcaggcga caacaggtta actgtaaggt tattacggat tcacaaacgt
    48961 cgcttttacc cgcgacgagc gtttgaaata ggggacgatc aatgcggttg ccaacaaagt
    49021 aaacagcaac tggcgaacgg ctatgccatc ttccacgggt gaaaaagcga atgcttttac
    49081 tgccagcaat acggaaataa gcagccagat aatatagtgt ttaggaacac aacgccgacg
    49141 tttaaagaaa gcgatggtca accacagtgt gtaataccac atggcgatag cggtaataaa
    49201 agaaacgccc cataagattt gtgtcgtcag tgcttgtccg ccgagcgttt gaaatgtttg
    49261 cggagaagat aacgcagcgg tatacagcaa caacgccagc gtcgtactca acagagcgac
    49321 taaaagccag gccaacgggc caagcaacca gcctccaata cgttgtggcg ttgtggtggt
    49381 cataggttct ccagaatagc gacgcaaaca ggcaaatcta attttagggg cgttattata
    49441 aggcttttat aaccgatcgc tactttcttt attggacata gtgccagacg gacttcggta
    49501 cctggccgag gtcaaacaaa cgcccgccag aaaccagctc tgcgcgacgg tgatcggcag
    49561 cacgatacat attgatcagt tcctgatcgt ccgtcagggt gtaattaaga tggtcgtaga
    49621 gtttttccag actttcgagt gaactgattt tgcgaaattt taataagtag tcctgaac
//
LOCUS       DAPHJE010000017        49521 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPHJE010000017 DAPHJE010000000
VERSION     DAPHJE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.017"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(166..1065)
                     /gene="pgrR"
                     /locus_tag="RH222_001022"
     CDS             complement(166..1065)
                     /gene="pgrR"
                     /locus_tag="RH222_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV3796077.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1203..2123
                     /locus_tag="RH222_001023"
     CDS             1203..2123
                     /locus_tag="RH222_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3796078.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2194..2331
                     /locus_tag="RH222_001024"
                     /pseudo
     CDS             2194..2331
                     /locus_tag="RH222_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            2459..3187
                     /gene="mpaA"
                     /locus_tag="RH222_001025"
     CDS             2459..3187
                     /gene="mpaA"
                     /locus_tag="RH222_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV3796079.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCANPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(3162..4127)
                     /gene="ycjG"
                     /locus_tag="RH222_001026"
     CDS             complement(3162..4127)
                     /gene="ycjG"
                     /locus_tag="RH222_001026"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV3796080.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADIDGPTWLAVDVEPALQFTTGELHL"
     gene            4246..4752
                     /gene="tpx"
                     /locus_tag="RH222_001027"
     CDS             4246..4752
                     /gene="tpx"
                     /locus_tag="RH222_001027"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV3796081.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(4796..6337)
                     /gene="tyrR"
                     /locus_tag="RH222_001028"
     CDS             complement(4796..6337)
                     /gene="tyrR"
                     /locus_tag="RH222_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV3796082.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6485..7546)
                     /locus_tag="RH222_001029"
     CDS             complement(6485..7546)
                     /locus_tag="RH222_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV3796083.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(7543..8940)
                     /locus_tag="RH222_001030"
     CDS             complement(7543..8940)
                     /locus_tag="RH222_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV3796084.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            9095..10093
                     /locus_tag="RH222_001031"
     CDS             9095..10093
                     /locus_tag="RH222_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3796085.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(10204..11109)
                     /gene="ompG"
                     /locus_tag="RH222_001032"
     CDS             complement(10204..11109)
                     /gene="ompG"
                     /locus_tag="RH222_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV3796086.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11154..12236)
                     /locus_tag="RH222_001033"
     CDS             complement(11154..12236)
                     /locus_tag="RH222_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3796087.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLHVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIALR"
     gene            complement(12250..12909)
                     /gene="pgmB"
                     /locus_tag="RH222_001034"
     CDS             complement(12250..12909)
                     /gene="pgmB"
                     /locus_tag="RH222_001034"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV3796088.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(12906..15173)
                     /gene="ycjT"
                     /locus_tag="RH222_001035"
     CDS             complement(12906..15173)
                     /gene="ycjT"
                     /locus_tag="RH222_001035"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV3796089.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15170..16225)
                     /locus_tag="RH222_001036"
     CDS             complement(15170..16225)
                     /locus_tag="RH222_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3796090.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(16235..17023)
                     /locus_tag="RH222_001037"
     CDS             complement(16235..17023)
                     /locus_tag="RH222_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV3796091.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(17041..18093)
                     /locus_tag="RH222_001038"
     CDS             complement(17041..18093)
                     /locus_tag="RH222_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV3796092.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(18124..18966)
                     /locus_tag="RH222_001039"
     CDS             complement(18124..18966)
                     /locus_tag="RH222_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV3796093.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(18953..19834)
                     /locus_tag="RH222_001040"
     CDS             complement(18953..19834)
                     /locus_tag="RH222_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV3796094.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(19855..21147)
                     /locus_tag="RH222_001041"
     CDS             complement(19855..21147)
                     /locus_tag="RH222_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3796095.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(21161..22840)
                     /gene="ycjM"
                     /locus_tag="RH222_001042"
     CDS             complement(21161..22840)
                     /gene="ycjM"
                     /locus_tag="RH222_001042"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV3796096.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(23053..23367)
                     /gene="pspE"
                     /locus_tag="RH222_001043"
     CDS             complement(23053..23367)
                     /gene="pspE"
                     /locus_tag="RH222_001043"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV3796097.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(23442..23663)
                     /gene="pspD"
                     /locus_tag="RH222_001044"
     CDS             complement(23442..23663)
                     /gene="pspD"
                     /locus_tag="RH222_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV3796098.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(23672..24031)
                     /gene="pspC"
                     /locus_tag="RH222_001045"
     CDS             complement(23672..24031)
                     /gene="pspC"
                     /locus_tag="RH222_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV3796099.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(24031..24255)
                     /gene="pspB"
                     /locus_tag="RH222_001046"
     CDS             complement(24031..24255)
                     /gene="pspB"
                     /locus_tag="RH222_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV3796100.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(24309..24977)
                     /gene="pspA"
                     /locus_tag="RH222_001047"
     CDS             complement(24309..24977)
                     /gene="pspA"
                     /locus_tag="RH222_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV3796101.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            25144..26121
                     /gene="pspF"
                     /locus_tag="RH222_001048"
     CDS             25144..26121
                     /gene="pspF"
                     /locus_tag="RH222_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV3796102.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(26252..27517)
                     /gene="puuE"
                     /locus_tag="RH222_001049"
     CDS             complement(26252..27517)
                     /gene="puuE"
                     /locus_tag="RH222_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV3796103.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(27555..28835)
                     /gene="puuB"
                     /locus_tag="RH222_001050"
     CDS             complement(27555..28835)
                     /gene="puuB"
                     /locus_tag="RH222_001050"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV3796104.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKMLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(28837..30324)
                     /gene="puuC"
                     /locus_tag="RH222_001051"
     CDS             complement(28837..30324)
                     /gene="puuC"
                     /locus_tag="RH222_001051"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV3796105.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(30474..31031)
                     /gene="puuR"
                     /locus_tag="RH222_001052"
     CDS             complement(30474..31031)
                     /gene="puuR"
                     /locus_tag="RH222_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV3796106.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(31058..31822)
                     /gene="puuD"
                     /locus_tag="RH222_001053"
     CDS             complement(31058..31822)
                     /gene="puuD"
                     /locus_tag="RH222_001053"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV3796107.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            32034..33452
                     /gene="puuA"
                     /locus_tag="RH222_001054"
     CDS             32034..33452
                     /gene="puuA"
                     /locus_tag="RH222_001054"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV3796108.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            33601..33765
                     /locus_tag="RH222_001055"
     CDS             33601..33765
                     /locus_tag="RH222_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796109.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            33755..35140
                     /gene="puuP"
                     /locus_tag="RH222_001056"
     CDS             33755..35140
                     /gene="puuP"
                     /locus_tag="RH222_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV3796110.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            35274..35519
                     /locus_tag="RH222_001057"
     CDS             35274..35519
                     /locus_tag="RH222_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV3796111.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            35832..37475
                     /gene="sapA"
                     /locus_tag="RH222_001058"
     CDS             35832..37475
                     /gene="sapA"
                     /locus_tag="RH222_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV3796112.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            37472..38437
                     /gene="sapB"
                     /locus_tag="RH222_001059"
     CDS             37472..38437
                     /gene="sapB"
                     /locus_tag="RH222_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV3796113.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            38424..39314
                     /gene="sapC"
                     /locus_tag="RH222_001060"
     CDS             38424..39314
                     /gene="sapC"
                     /locus_tag="RH222_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV3796114.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            39314..40306
                     /gene="sapD"
                     /locus_tag="RH222_001061"
     CDS             39314..40306
                     /gene="sapD"
                     /locus_tag="RH222_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV3796115.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            40308..41114
                     /gene="sapF"
                     /locus_tag="RH222_001062"
     CDS             40308..41114
                     /gene="sapF"
                     /locus_tag="RH222_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV3796116.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            41182..41535
                     /locus_tag="RH222_001063"
     CDS             41182..41535
                     /locus_tag="RH222_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDV3796117.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            41903..42691
                     /gene="fabI"
                     /locus_tag="RH222_001064"
     CDS             41903..42691
                     /gene="fabI"
                     /locus_tag="RH222_001064"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV3796118.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            42835..43962
                     /locus_tag="RH222_001065"
     CDS             42835..43962
                     /locus_tag="RH222_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV3796119.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            44030..45964
                     /gene="rnb"
                     /locus_tag="RH222_001066"
     CDS             44030..45964
                     /gene="rnb"
                     /locus_tag="RH222_001066"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV3796120.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(46042..46146)
                     /locus_tag="RH222_001067"
     CDS             complement(46042..46146)
                     /locus_tag="RH222_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796121.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            46200..48185
                     /gene="pdeR"
                     /locus_tag="RH222_001068"
     CDS             46200..48185
                     /gene="pdeR"
                     /locus_tag="RH222_001068"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV3796122.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            48332..48505
                     /locus_tag="RH222_001069"
     CDS             48332..48505
                     /locus_tag="RH222_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796123.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            48595..49344
                     /locus_tag="RH222_001070"
     CDS             48595..49344
                     /locus_tag="RH222_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3796124.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT        11747 a        13140 c        12431 g        12203 t
ORIGIN      
        1 atagaacacc gaacactacg ttcaggcttc gcacgttcaa accagttttc ggtatcataa
       61 tcagcatctg tcgccattgt acgaagaata aaaaataacc aaaacgttga aaggaactga
      121 gaatacgtta agagagaaat taaattaatg tatcagcaag tagtgctaaa cagcaccttt
      181 atacttcagc ctatctataa ataatgagaa tgccgagcca gcatgtcgac ggtgtggata
      241 gtacaggtga taaccgggta aatctggtgt gaatttatcc aaaacacgta tcagtttttt
      301 ttctttaata gcccgctcaa cctgatcata aggtagatac gccaatccat gcccatcaat
      361 tgcagcatca atgatcaggt ctatcgtatt cagtaaaagc tgaccttcca tgcgaacacg
      421 aacttcacgt ccaccgcgta ttaatctcca gcgatttgct gtacccgatg tgggaagata
      481 caaattaatt gcctgatgat ctattaattg tgacactgac gttggaacac ttcggcgaga
      541 aaaataatcc ggtgagccaa caatagccat tggtatatct ggcccgattc gaatggcgat
      601 catatcttta tccatctccc cacccagacg gacgcctgca tcaaaacgtt cagaaacgac
      661 atcggtcaaa ccataatcaa tggtgagctg aatatcaatt tcaggatgcg atttcaggaa
      721 tgtgcgcatt gctggtaaca atatcgtttt tgctgcatgt tctacagtag taatacgtat
      781 tgtcccggat gggcggttct gcagctcgct cagggatgcc agggctgaat ctatgtcatg
      841 caacataggg ccaagaacag acaaaagatg ctcgcccgct tcagttggaa caacgctgcg
      901 cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct atacgacgca ctatctggct
      961 taaagctgac tgcgccatgc tcaggcgggc tgctgcacga gtgaagctac gctcctctgc
     1021 aacgacgaca aacgccatca gatcagcgat ttcttcacgc ttcattcagt atctccatgc
     1081 aatctattaa tcatttaaat gataggtcta atcatgattt tgggtctaat caacttaatg
     1141 atcaatatct atagtgatat caccaaccgt taaaacgaac cgacaactcc atgaggaacg
     1201 gaatgatgaa taataaagtc agcttcacta acagcaataa tccaaccatc tctttgtctg
     1261 cagtgatcta tttccctcca aaatttgatg aaacccgtca gtatcaggca attgtggtat
     1321 ctcacccagg tgggggcgtt aaagaacaaa cggccggaac ctatgccaaa aaactggcgg
     1381 aaaagggatt tgtcactatt gcttatgacg catcttatca aggtgaaagt ggcggcgagc
     1441 cgcgtcagtt agaaaaccca tacattcgta ctgaagacat cagtgccgtg attgattacc
     1501 tgacaacgct ttcttatgtc gacaataccc gaattggtgc gatgggaatt tgtgccggtg
     1561 caggatatac agccaatgct gcaattcagg atcgccgcat caaggccatc ggtaccgtta
     1621 gtgcggtcaa tattggttcg atgttccgta atggttggga aaacaatgtg aaatctattg
     1681 atgccctgcc atacgttgaa gcgggttcaa acgcccgaac cagcgatatt agcagcggag
     1741 aatatgccgt tatgccactg gcaccaatga aggagtccga tgcgccgaac gaagaactgc
     1801 gtcaggcctg ggagtattac cacacccctc gcgcgcagta tccaacagca ccgggttacg
     1861 ctactttgcg cagccttaac cagattatta cctatgatgc ttaccatatg gcggaagtgt
     1921 acctgactca gccgatgcag attgtggcgg gcagccaggc agggagcaaa tggatgagtg
     1981 acgacctgta cgatcgagca tcaagccagg ataaacgcta tcacattgtt gaaggcgcaa
     2041 accatatgga tctttatgac ggcaaagcct atgtggctga agcaatttca gtattagctc
     2101 cgttctttga ggaaactctg taatgaaaca tattcaaatc cgcaatagtg atatggcctg
     2161 gcatattgct gccaataatc tgggataatt ttcatgaaaa aggtactgat tcttggtgct
     2221 ggcggtcaga tagcccgcca tgtgattaat caactggcag ataagcaaac cattaaacaa
     2281 actttatttg cccgtcagcc agcaaaaatc cacaaaccct atccaactaa taaaatgcaa
     2341 acaacttccg gcaaataaag tgatacaaga tcgatgacat agcgttgaag aaaaggattt
     2401 gataatccgc aaaatttgca caacactgcc tgtaatactt cctgaaccag agacgatcat
     2461 gaccgtaacc cgcccacgcg ccgaacgcgg cgcatttccg cccggaacag aacattacgg
     2521 acgttcatta ttgggcgcgc cgttaatctg gtttcctgcc cctgcagcca gccgcgaaag
     2581 tggtttgatt ctggctggca ctcacggtga tgaaaactct tcggtcgtca cgctctcctg
     2641 tgctctgcgg acgttgacac cttctttgcg ccgccatcat gtggtgctgt gtgcgaatcc
     2701 tgacggctgc cagctggggt tacgggccaa tgctaatggt gtggatttaa acagaaactt
     2761 tccggcggcg aactggaagg aaggtgaaac ggtttatcgc tggaacagcg ccgctgaaga
     2821 acgtgatgtc gtattactga caggtgacaa acctggctca gaacctgaaa cccaggcgct
     2881 gtgccagctt atacaccgca ttcaacctgc ctgggtggtc tcattccacg atccactggc
     2941 ctgtattgaa gatcccagac acagcgaatt aggcgaatgg ctggcccagg cgtttgaatt
     3001 gcctctggta accagcgtcg gttatgaaac gcctggctct ttcggcagtt ggtgtgcaga
     3061 tctcaacttg cattgcatca ccgcagagtt tccgcccatc tcctccgacg aagccagcga
     3121 aaaatatctc tttgcgatgg caaacttgct gcgctggcat cctaaagatg caattcgccc
     3181 gtcgtgaact gaagcgccgg ttccacatct accgccagcc aggtcggtcc gtcaatatcg
     3241 gcgaaactga cctgcggcac cagcggtaaa gcggcgctaa tggcacgaga ggtacacaac
     3301 atgcagccca gcatcagact gaaaccttgt gcacgcgctt cagtcgccag cgccagcgct
     3361 tccgtcagac caccggtttt atcgagctta atgttaacca tctcatagcg ccctttcagc
     3421 gccttcagat tgctacgtgt atgacaactc tcatcagcac aaatcggcaa cggatgaata
     3481 aaattctcca gcgccgcatc gtcctgcgca ggaaggggtt gttcaagcat cgcaacgcct
     3541 aaatccgcca atagctggca acgcgccgcc aacccttctg cacgccagga ttcatttgca
     3601 tcaacgatca gcgtcgcatc gggcacagct gtgcgaattg ccaccatccg ctcactaata
     3661 agatggttat ccagcttcac tttcagtaat ttcgcgcctg cctgccagag tgttgatgca
     3721 ctattggcca tctgatcagg cgtaccgatg actaccgtct gtgcagtgat aactgtctcg
     3781 ggaagcgtta tgccgatcaa atcagccagc gattgctgct gttttcgcgc ggccagatcc
     3841 cacaatgcac aatccagcgc attacgtgct gcgccggcag ggagaatttt ttgcaactcc
     3901 tcccgtgtca gccctttctc taattgcgac acgacgctca taatttgcgc cattaccgag
     3961 gcatcacttt ctccataacg cggatacggc gtgcattcgc cggtgccttt aataccctct
     4021 tcttccagtt caaccaccac cacgcgcgct tcactgcgac ttccccgggc aatcacaaac
     4081 ggggtatgta agggccaggc ttcctcaaat accttaacgg ttctcatctt tactccttgt
     4141 tgtcgcggtg gtctgcagat taagcgcaaa tagcgtttgc tgtgttattg acagttagca
     4201 taaactaggt gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc acaaaccgtt
     4261 catttccagg gcaacccggt tacagtcgcc aattccatcc cgcaggcggg tagcaaagcg
     4321 cagactttta ctctcgtggc aaaagatctg tctgacgtca ccctcggtca gtttgcgggt
     4381 aaacgcaaag tgctgaatat tttcccgagt attgataccg gtgtttgcgc cgcatcagta
     4441 cgtaagttta accaactggc aactgagatc gacaacaccg ttgtgctgtg tatctctgcc
     4501 gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa cgttatcacc
     4561 ctctccactt tccgtaacgc tgaatttctg caagcttacg gtgtggcaat tgctgatggc
     4621 ccactgaaag gtctggcagc gcgtgccgtt gtggttattg acgaaaatga caatgtgatt
     4681 ttcagccagc tggtggatga aatcaccacc gagccggatt acgaagcagc tctggctgta
     4741 ctgaaagcat aatttaatat gcctgatggt gttgcaccat caggcatatt cgcgcttact
     4801 cttcgttctt cttctgactc agaccatatt cccgcaactt attggcaatc gcggtatgtg
     4861 aaacgccgag acgttttgcc agtttgcgcg tgctgggata attgcgataa agctgggtta
     4921 ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga accttccatc gcatcttcgc
     4981 ccacggctac cgttgcggcg tcataatccg gcaacaaaat atcctgtgga cgcagctcat
     5041 aaccgtccag ttgtgtcagt gcgcgataga tagcgttctt taactgccgc acatttcccg
     5101 gccacgcata acgcgtaagt acagtattca ggtcagcggc cagtttcgga cgcggcacgc
     5161 cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa cggcatgatg tcctgcggac
     5221 agtcacgtag cggcggcaga ttgagcgtca acacgttcag acgataatag agatcttcac
     5281 ggaacatgcc tttttgcacc agttcgacca gattcttctg cgtagcgcaa atcacccgca
     5341 catcgacatg cacctcatgg tcttcgccaa cccgacggaa agtgccatca ttaaggaaac
     5401 gcagtaattt cgcctgcatc cgtggtgaca tttcccctat ttcatccaac agcaccgaac
     5461 caccgttcgc ctgctcaaag aatcctttct tcccttccgg agcatgacca aacagttcac
     5521 tctcgaccgc atcttccggt atagacgcac agttcagcgc caggtaaggt ttgcccgctc
     5581 tggggcttgc ctgatggcag gcgtaggcaa agagatcttt acctgtacct gtgtcacccg
     5641 taatcagcag cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca acatgcttca
     5701 ttttcgggct gacggcgaca atttgactga aggcgctgac gtcctgggcg gcgacatttt
     5761 gcaactggcg gcccatacga atcgttgatc gcaacatcac caccgcaccg gtcaggacgt
     5821 gttgatcatt ttcatcctga agataaacag gcgtaatctc catcaggaaa ttctgcccat
     5881 taataacgac atgctcgtta tgcgaatctt gcggttcgct ttccagccaa cgtaaaaaat
     5941 taaagccgtt aatcaattgt gcggcggtat ggttgcgcag gcgatccaat ttttgcccaa
     6001 aaagctgaca gctcgccggg ttcgccatat ccactttgct tttcatatcg acagagagca
     6061 caggttcagg caacgcctcc agtaacgcgc tcaacgccag atgctcacgt tcggaaggca
     6121 tccacgggac agtacgcaca tcggtaacac ccgcaatacg gcgtatttcg gccatcagac
     6181 tgctgaaact ctcaaactcc agttcagcaa aattgaggta gattcgccca atgggatcaa
     6241 tctcaatacc gcgtaaatca atgcctctta gcacgagtag atcgagtaat tcgcgggtca
     6301 gaccgagtcg gtcttcacaa aagacttcca gacgcatggg aaccttcacc tgaaaaaaga
     6361 acaattaata tgatgatatg acactatttg gatagcagga aggtttctgt caacaatcat
     6421 tgacacaaag acggaaaaac gggcacttcg gcgtaaagat atccggtcag cacccgatat
     6481 tgcattattt ttcgctgggc gttttgcctt tttgcagcgt ttctttcacc tgaccgataa
     6541 gctgacgacg gaaatccccg aggcgaggtt tgtcatcgtc aatccacggc agcgggcggc
     6601 aaagctccat agctttaatc ccgagtcgtg ccgtcagaag tcctgcacca atcccctgag
     6661 ctgcgcgggt agacaaacga gcagcgagat cttgcgacat ccagtccatc cccacttcgc
     6721 gcaccagttc gctggctccg gcaaaagcga tattcagcaa taccagctta aacagacgca
     6781 aacggctgta ataccccagt tcaatgccat acagcgtggc gatgcgatta attaaacgca
     6841 gattgcgcca ggcgataaac gccatatcga ccaacgccag cgggctgacc gcaatcatca
     6901 acgttgattc cgccgccgaa cggctgattt cgcgccgcgc ctgggcatct aaaactggct
     6961 ggaccaaatg cgcatacagg ctgaccactt cacggtcgtt ttgcgtttca tggattgagg
     7021 cataccagcg ttgcagcgcc ggatgcgact gatcaatacc cgcctgctgc gccagttttt
     7081 cgcaaaatgc gcggcctttg cccgtgccgt ggctatgcaa taaatcacgc gcttcgtcgc
     7141 gttcatgggc gcgctgtcgc aagcgccata agcgccgcca ctctgttacc acagaaccta
     7201 cgccagcgcc gataatcaat gccccagcgg cacatccacc cagcgccacc cagtcctggg
     7261 tttgccaggc attcattgtc cactgtacac cctgcccgac aacgcttgcg ccaaacagag
     7321 ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt cggacgtaac gctgcgtcca
     7381 ttaccgcttc gacttgcccc tcttcttcct gcgcttcgtc gagcgtggcc ggggcaaaat
     7441 tttgcgcctg attttcgtca aaggtctgct gcgccctgaa tttaggattc tgatcgacct
     7501 ccagaggacc gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca atttatctcc
     7561 tattaaaaat tccagcgcag catcaagacg aatatgcggt agtggtttgt cgacatccat
     7621 cacctgcgga cgaaatgcct caaactggaa gccttgctta tcccagaacg cctgaccagg
     7681 caaacgtgcg ggaacttcgc caggataaac agtgagcggt gcgccatcgc taagtcgatt
     7741 accacgcagc gccgggattt tctcaccgtt aacatcaata atgccgctgg tggtcgcctg
     7801 aactgacgcc agccccaggc agtccatgct gatcccttcg aacgccgcat tttgccaggc
     7861 atcctgaatc agttgttgca gtaatgaaac catattagcg tgctgatcga tggtcacatg
     7921 gtccgcttta gtggcagcaa acaatagctt atcgataacc ggcgaaaaca aacgccggaa
     7981 cagggtacgc tgcccgtagt gaaaactttg catcagctgc gtcagtgcca gacgcatatc
     8041 attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa tccaccagca caatctggcg
     8101 gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc accttctcgc agtaataatt
     8161 aaagcgctcg cgcagcattc cggcattggt atgcttatcg gcctgcgcca gtttggactc
     8221 gccccaggta tcgacatccg gccacgggaa gaattgcagc gcgggcgcac ctgccatatc
     8281 tcccggcaag acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt tacagtggtg
     8341 gagataatcg gtccacgcgg cggcaatgtc cgccagccgg ttttcgtcgg caggcgctag
     8401 cgggtcaagt ccttcgcaca tcattcgcca tttcgccgac cattcgccgc gctgaccatt
     8461 gagtaagccc gtcatctggc gcgaccagct taagtaatcc tgcgccagca tcggcaggtc
     8521 gagcaaccat tcgccgggat aatccacaat ctccagatac agcgtggagg tgtccttgaa
     8581 gtggcgtagc agcgaatcgt tcgatttaaa acgtagcgcc aggcggattt cactgacgcc
     8641 gcgcgttggc gtcggccagg cgggaggatc gccatataac tgcgccagtc cttcatcata
     8701 tgtgaagcgc ggaatgccaa agtcacgctg aggaatgcgt tttacgccca gcaggcgctc
     8761 ttcacgcacc gcgcttaaca gcggcaagcg tgctccggcg tgaatattga gcaactgatt
     8821 gaccatcgca gtgataaacg ctgttttgcc gctgcggctt agtccggtta cggcgaggcg
     8881 cagatgtctg tcgacacccc gattcaccag cgcattaagt tcatttttaa gtcgcttcat
     8941 tgctgtcctg tgctgctctg aagaccagaa aaaaatcatt cgtaaggata tgaatgtggg
     9001 ggccaggcca ttgattatca actgcccgtg ttgtcatcga cgggcgaact ccataagatg
     9061 tgcagcattc acgttcggac ggagttcctg acctatgtcc cctactattt atgatattgc
     9121 cagggttgca ggcgtatcaa aatccaccgt atcacgcgtg ctgaataagc aaactaatat
     9181 ctccccggaa gcgcgcgaaa aagtgttacg ggccattgaa gaattacagt atcagccaaa
     9241 caagctggcc cgcgcgctga cctcttcggg ttttgatgcc attatggtga tttctacccg
     9301 ttcgaccaaa actacggcgg gtaatccgtt tttctctgaa gttttgcatg ccatcaccgc
     9361 caaagctgaa gaggaaggtt tcgacgtgat attgcagacg tcgcacaatc cggcagaaga
     9421 cttacaaaaa tgtgaaagca aaattaagca gaaaatgatt aaaggcatta ttatgctaag
     9481 ttcgccagcg gatgagtcat tttttgccca acttgataaa tacgatattc ctgtcgtggt
     9541 gattggcaaa gttgaaggtc aatatgccca tgtttattct gtcgataccg ataattttgg
     9601 cgacagcatt gcgttgaccg atgcattaat tgaaagtggg catcaaaata ttgcctgcct
     9661 gcatgctccg cttgatgtcc atgtttcagt ggatcgggta aatggttata agcagagcct
     9721 ggctgcgcat aatattgcag tgcgtgatga atggattgtt gacggcggtt atacccatga
     9781 aacagcactg caagccgcgc gggaattatt aagccagtca ccgttgcctg aggcagtgtt
     9841 tgctactgac agcctgaaat taatgagtat ttatcgtgcg gcagcagaga aaaatattgc
     9901 tattccgcag cagttagcgg tggtgggtta tagtaatgaa acgctgtcgt ttattttaac
     9961 gcctgcgcca ggcggtattg atatcccaac acaggagtta ggtcaacaaa gctgtgagtt
    10021 attattccgc ttaatttctg gaaaaccgtc accacagaat attaccgttg ccacgcatat
    10081 gacgctgaaa taaataccag ctaccttgcc tgaatgccgg atgcgatgct agcgcatctt
    10141 atccggccta catttatagt tcggataaag tactcgtgcc gcatccggca atttagccca
    10201 ttatcagaac gagtaattta cgccgacacc tgcataatgg aatttatcgc tgtcgccttc
    10261 gtcgtgatcc tgccactcaa acgcgtattc cagcgaaacg gaaagcccgt tctggaaatc
    10321 ataaccgtaa aataaaccta cacggttaaa atcatggcct tcacgttcaa tatcatcctg
    10381 ccagtcccag ttactccagc gatccagccc aatgcgcgta tacggcgtca ccgagtggtt
    10441 gccgagcgtc agcggcaaat aggcgcgaat ttcttgcgta gaaaactcac cgttattgcg
    10501 gctgtcgtcc atattaaagc cgcgctcgag ataatagttc actcgcaagg caaccgtttc
    10561 gttgaaggta tattgcaggc ctgtttccgt ttcgactcgg gtatcagcgt aaccggtcgt
    10621 gttcagatcg ttggcaaatt tatacatcga taaccaaccg ttgaaacgta aatcgtccgt
    10681 cagtttcaca tcccagtctg gcgcgatttt ccagcgctgc atattcgccg tgtctttacc
    10741 cggttcatca acgtagtgat aaccataatt acggaaacca ccggtcaggc cgaaactgaa
    10801 atcatcgttt tcgaggaact gataatgcac ctccagctcc gggcgatcaa accacgttcc
    10861 acgtttaccc gcgctataat ctaccggccc ttcctgataa taggccagag caattctcca
    10921 cggcccgttg gcggcattaa aatagactga aggctccgcc agcccatcca tatcttcgcc
    10981 ataaccctcg acgttttcta tttcgtacat cgcgccgata ttaaagtgcc agtcgttctt
    11041 ttcctcggcc tgtgcgcagg ccattcccgc acacatcacc agtgcggtac agggtaataa
    11101 ctttttcatt attgttcctt gtattatcct gggggaatgc cttccccctg tatttagcga
    11161 agtgctattt ccgtttctgc atcaaagaaa tgacatttcg tcatatcaaa atgaatagtg
    11221 atattttctc ctgcatgata atcatttaac gcaccagcac ggaccactaa ctcgtgcccc
    11281 ccaaccgtgg tgtagagcat aaattccgca ccggttaatt ctgccacgct aattttggcg
    11341 gaaatgttat tttcctcttg cgcgtccgga tgaatatctt ccggacgtat tcccatcacg
    11401 atgggcttat gcaaacttcc ctgtgttttc agaaccgcta atttctcttc gggaatggtt
    11461 aatttaagcg tttccgtaac gaatttatcg ccatcgatcg tgccacgaat aaaattcatc
    11521 gccggtgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
    11581 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
    11641 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
    11701 ttgctgatct ctgcgcgcat ttgcacgtgc agcttggcat caaggttaga gagcggttca
    11761 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
    11821 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
    11881 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
    11941 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
    12001 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
    12061 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagccc
    12121 gacgggccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
    12181 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
    12241 attcctttgc tatacgtttt gccagaaggc cgataaccgc ggccaggtga gtgattccgt
    12301 tgaaggcaac agtaattgcg ccccggttaa gcccgcgccg atccccaccg agcgcatacc
    12361 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
    12421 cggcacgccc agcccggcac aggcggcgag aaagatttcc gggtccggtt tcgagttttt
    12481 aagttgggaa gcatccgcgc agaaggtgaa aaactcgcgc agctccagcg ccgctaaaat
    12541 cgtcggcgca ttcagggaga cagaagccag cccaaccggg atctgctgtg cacggagatc
    12601 tgccagcaaa gagcgaatgc cgggtagaac agcgctgacc gtcaactcgc gcagtgagtg
    12661 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
    12721 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
    12781 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
    12841 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
    12901 cagtttcatt catcctcctg atgtgtggta gcggtcccat tgaagggcaa aataaaatca
    12961 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
    13021 acgggcgcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
    13081 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
    13141 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
    13201 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
    13261 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
    13321 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
    13381 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
    13441 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
    13501 agaatggttt gcttccccgc cgccgctttg tatttcgcca gattaatcgc cggcttagcc
    13561 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
    13621 ttaaggaaca tttcggcgcg atggataaac gcatcgtcgc tacagccaaa ctggcgggca
    13681 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
    13741 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
    13801 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
    13861 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
    13921 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
    13981 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
    14041 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
    14101 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
    14161 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
    14221 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
    14281 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
    14341 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
    14401 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
    14461 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
    14521 ctggtatgtt gctgcaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
    14581 accatacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
    14641 tgcatcagat gctgaccaaa cacccgcacc tgggtttcgt cgagatgttg gcgaccgtgg
    14701 ttggtttgcg tggcgtcgat acctgttgaa atcagcactg acgcgtcggc gtccagtggc
    14761 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
    14821 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
    14881 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
    14941 atggcaatct ccatccccaa gatatcaggc aggttcacca gttcgttgat ttcacctttt
    15001 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
    15061 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
    15121 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
    15181 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
    15241 cctgctcggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
    15301 tgctgcgcaa atggcggttg tcgtctgcca gttcccgttg catcagcgtc attaattcac
    15361 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accggcttta tcaccacaaa
    15421 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
    15481 gaatgccgcc gttatgaaat tcaattgtgc caaacagcga atcttcgacg ctataagttg
    15541 ccggatccca ctcgccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
    15601 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
    15661 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacgcccc
    15721 agccaggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
    15781 cgccgttggt cacctggtca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
    15841 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
    15901 gcgtcatggc gggcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
    15961 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac tacatcaggt ttcaccgcca
    16021 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
    16081 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
    16141 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
    16201 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
    16261 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
    16321 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
    16381 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
    16441 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
    16501 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
    16561 tatgatcctg atagcggttt aacggttcga gatacaccac ggttccggta cgcgcggcga
    16621 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
    16681 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
    16741 ctttaccccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
    16801 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
    16861 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
    16921 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
    16981 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
    17041 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
    17101 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
    17161 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
    17221 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
    17281 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcgacat aagagatggt
    17341 gccgccataa gcaaggccac gcagcgccga ttgcagcgcg tcggcgtaac cgctggtttc
    17401 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctcttttc ctacgtcagt
    17461 gccaatgggg ttaaggcaga aatcagcacc gtggcgacgg gcaatatcac agcgataggc
    17521 gataggatcg acgccaatca ccaccgacgc gccagcgcgt ttcgccagtt ggatggctat
    17581 ttgaccaatc gcgccaagcc ctaccaccac cacaaaatca cccacgcgca cgttggcatc
    17641 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
    17701 gccttgcggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
    17761 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
    17821 ctcgataatg tcgccaacca ccatgttgcc aagctggaat ttgccaaact caatgccgcg
    17881 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
    17941 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
    18001 cacgcggatt ttcacttcat tggctaaaat cgcccgatct tcatactcaa ccagcgcagc
    18061 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
    18121 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
    18181 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
    18241 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgctggcgct
    18301 ggagaggaag atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
    18361 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
    18421 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
    18481 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
    18541 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
    18601 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
    18661 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
    18721 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
    18781 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
    18841 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
    18901 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
    18961 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
    19021 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
    19081 gatagacgcc gagaatatcg accttggtgg tcagcaaata aacatccgcg aacatgtaga
    19141 acatccagat ggtgcgcagg gtcaccaccg tcgccaggac cggcataatc gcgggcagcg
    19201 tgacgatacg aaaccgttgc caggcattag cgccatccat ctctgccgct tcgtacagcg
    19261 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
    19321 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
    19381 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
    19441 tgaacatgta tttccaggcg aacaccagcg aaatggacgg cgttacgtag gagaggatca
    19501 ccagcgaacg cgcggttttg cgcaggcgga actcgcgatt gaaaaacatc gccaccgcca
    19561 gcccgagcac ggtgctgccc gccaccacca gcgcggtgta ccagaccgtc atccacagtg
    19621 agtgccagaa gccgggatcg gagaggatac gcacatagtt gctcacccca acaaaggttg
    19681 actcgatgtt gggattgagc ggcagacgta aaaaactaat ttcgatattc gacaccatcg
    19741 gccacgccac cagaccgccc agcagtaata agctgggcgc gagaagcaac agcgcaaagg
    19801 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
    19861 ctgttcgacc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
    19921 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaccccg tcacatcacc
    19981 catgcgggta aagtttttat cccctactgc accaaagacc tgaatatttg gcagttcacc
    20041 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
    20101 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
    20161 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
    20221 ggtcttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
    20281 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatca cagccggaag
    20341 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
    20401 catgatgtcg ttagaacccg gcatagtgtt ggcagcaagg tcgcggtaat aggtcagtgc
    20461 ctgcatcatc tctggtgtat caagggtgat tttgccttcg gcgttaaaga cgttagcctg
    20521 gttggataac gcaaactggg agaaggattg ttccgtcaac acgctttctg ctgtaggcag
    20581 cgcaatgccg tattttttat tcgccgggtc attcagtttc tgtgcaacgt ccagcagctg
    20641 ttgccagttt ttcggctcct caagccccgc ttttgccagc acatctttgc gataccagat
    20701 accgccaatc caggcgctga caggaacacc ggtccatgcg ctaccatctt cggtacgcac
    20761 aatacgcagt acgccatcgt aaaacgcgcc ttcaccaacg ttgctgatga ctgtggcaac
    20821 cgctttgcga tcgataagct gctctttgtc catcactttg gcgtagtcat ggctggtttc
    20881 gatcacttcc ggcagcgaac cgctacgtga aagagtaatg actttagtgt tataggcatc
    20941 ttcttccacg ggcacttgct taacggtgat gccagggttt tctttttcaa aacgggcaat
    21001 caatttactg ataacggcct ggcgctcctg ctccaccgaa gaatgcataa attcgatgga
    21061 tacattcgtt ttattttctt ctttacagcc tgaaatcagg gcgcaggaaa ccagtgctga
    21121 taacagcacg atttttgatt taatcatgag atgttccttt ttatttaatc cacataacct
    21181 gccaggggcg cagcgttatt tcattaccca atatatcaac ttcactaatc agatcccgac
    21241 catgcagatt agtaatattt acatgctgaa tatttttact gacattaaac aatccagtca
    21301 gacaattacc atcagcgtta cttctttgaa tacgcattac ggatgaatta atcgtatcaa
    21361 tggtaaaatt attatccgga tgaaactcgt tatggctgcg acgaagtgta attaaacgcg
    21421 acaactcatg atataccgca tgccttaatg tagcttcatc gttcagttct cgggttatct
    21481 ctttactgtg atatttttta cggttaatcg cacggttata tccgagtttt tcgacacctg
    21541 cgtaatcatt acgcgagcca agaatacttt gaatatatat cgctggcaca ccggggaaac
    21601 ttaacaaaat cgcatgggcg agaataaacc tggcgcaacg ttcttcatcg ctactctcac
    21661 ggcggcttaa cgcatccata taggtcacat ttatttcata cggactgcgt gtaccgtcgg
    21721 gattattttt ccagtttact aatgcacctt cctgctgtaa cgcctcgacc agctctaata
    21781 tttcgctttc aggcaacaag ccccgtagcg ggtttagccc gatgccatcg tgagaggcga
    21841 ggaagttaaa ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa
    21901 gcgcctcaac gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt
    21961 acaccatatg tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa
    22021 cattggtctc ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca
    22081 gtttgataat cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca
    22141 taaagccaac ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa
    22201 catccaccat cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac
    22261 taaaggtggt ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg
    22321 gtaacgcacg cgggcgagtg acggcgctga gatcggtttg cgggtcaacg gcaataaaaa
    22381 aatcttcaaa acctggatgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca
    22441 tatggttgca gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct
    22501 gccactcccc cgcttcactg gcgacctgtt gataatcaat taccgaaaag ccatcatcag
    22561 atgaccatgg ataaaacggc aacaaatgaa catgtgaaaa aatgctttgc agccattgat
    22621 ggtaaaactg attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat
    22681 aggtaattaa cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc
    22741 gtttcgcact ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac
    22801 cgtagatttc gtccaggtga tccgtaattt tctgtttcat gttgcctttc cgtggtagcg
    22861 gtcccatgaa acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt
    22921 tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat
    22981 aatctatggg gatgtaaata aggtgtgaca gaaaaaaaaa cggcgcataa gcgccgctca
    23041 tggtgaattc ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt
    23101 ctcaacgtgg gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc
    23161 ggcattgcaa tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc
    23221 tttcacttcc ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc
    23281 tggaacacga acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc
    23341 cagagcaagt aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc
    23401 cattataggc ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt
    23461 gccagtttat tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc
    23521 agcggtcggc gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc
    23581 agtgcggtaa gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg
    23641 gcctgttgcc agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg
    23701 tgaaagtatc ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca
    23761 gttcacggtc gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca
    23821 tgttgtccgg cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca
    23881 gcgccagacc gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat
    23941 caaaatagtt ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc
    24001 ataatttttt attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc
    24061 atcaagaatg gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag
    24121 ttgcgctaat cgctgctgct cactttgcga caattcactg cgaccagaac gattgctgta
    24181 atgtagccat aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag
    24241 aaatagcgcg ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct
    24301 ggatgttatt attgattgtc ttgcttcatt ttggctttta attgtgccag ttgctcgctt
    24361 attgcatcat cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag
    24421 ctgtggcttt ctgcttccgc ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga
    24481 gccattgctt catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt
    24541 gccgcctgat ggcgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt
    24601 tccagctcgc cgatctcttt cttcatgcgt gccagcgtat cgtccaccag cgtcacttca
    24661 tgttccagtg acttaatcag atcggtcagt ttctgttttt caattaacgc agcacgcgcc
    24721 aggtcttctc tatctttcag cagtgccagt tcggcttttt cctgccattc aacttcacga
    24781 gccgacgctt gttcaatgcg gcgagtaagc tgtttctttt ccgccagcgc acgcgccgaa
    24841 gtagaacgca cttcaaccag cgtgtcttcc atttcctgga tcatcagacg caccagtttc
    24901 tgcggatctt ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag
    24961 cgagaaaaaa tacccataat gttgtcctct tgatttctgc attcaggatt gtcctgctga
    25021 actgttaata caatttgcgt gccaattttt tatctttttg atttataaag atctgattga
    25081 agaatcaaca gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc
    25141 atcatggcag aatacaaaga taatttactc ggtgaggcga acagctttct cgaagtgctg
    25201 gaacaggttt cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc
    25261 accggtaaag agctgatcgc cagccgcctg cattatctct cctcccgttg gcaagggccg
    25321 tttatttccc ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt
    25381 cacgaagcgg gggcgtttac cggtgcgcaa aaacgtcatc caggaagatt tgaacgtgcc
    25441 gacggcggta cgctatttct tgatgaactc gctacagcac cgatgatggt gcaggaaaaa
    25501 ttattgcgcg tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccgttgcag
    25561 gtgaatgtgc ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggt
    25621 acttttcgcg ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccgccacta
    25681 cgcgaacgtg aaagcgacat tatgttaatg gcggaacact ttgccatcca gatgtgtcgg
    25741 gaaatcaagc tgcctctgtt cccgggtttt accgagcacg ccagagaaac attgctgaat
    25801 tatcgctggc cgggaaatat tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc
    25861 cacggcacca gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtcca
    25921 cctgaagaag ccatcgccgt ttcagaaaac acttcgcttc caacactgcc gctggattta
    25981 cgtgagtttc agatgcagca ggaaaaagag ttgctgcaac tcagtttgca acaagggaaa
    26041 tataaccaga agcgcgcagc tgaattgctg ggcttaacct atcatcagtt ccgcgcgttg
    26101 ttgaaaaagc accagattta gcgaagttgg gcaatacaac ttgccggatg cggcgtgaac
    26161 gccttatccg gcatacataa tcaatacgtc gtttgcaggt ctgataagcg cagcatatca
    26221 ggcattattg cgttgcccct gcttctgcgt attaatctct cagcgcatcc tgcaaaattt
    26281 tcattgccgc atcgaattgc gcatccggga tggtcagcgg atacaggaaa cgaatcacgt
    26341 tgccgtatgc gccacaggtc agtagaagca gtccctgcgc cagcgcgcgt tgctggattt
    26401 tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc gttaaactct gccgcaatca
    26461 tagaccccag accgcgtacc gccgcgatgg ccggaacgct ttctttggca tcaatcagcg
    26521 tgtttgtcag acgctggccc agttgattcg cgcgttcgca gagtgattct ttgtcgataa
    26581 tgttgagcac cgcgtgcgcg gcagccaccg ccagcgggtt accggcgtag gtgccgccaa
    26641 gcccgcccgg cgcgggtgcg tccataatat tcgcgttacc gaccacgccc gaaagcggca
    26701 tcccgcccgc gaggcttttc gccatcgtca ttaaatccgg cttatcggcg taatgatcca
    26761 tggcaaacag cttaccggta cgcgcaaagc cgctttgcac ttcatcagca atcatcacaa
    26821 taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc gcaacgttga
    26881 aaccgccctc gccctgcacc ggttcgaaaa taatggccgc gacctgcttc gcttcgatgt
    26941 ctgatttaaa caagcgttcg atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat
    27001 ctgacggata aggtacgtga tacaccgaac cagggaacgg gccgaagccg attttgtacg
    27061 gcgcgacctt tccagttaac gccatagtca tatacgtacg accgtggaag ccgccgctaa
    27121 acgcaatcac gccagggcgt ccggtatggg cgcgggcgat tttcaccgcg ttttccaccg
    27181 cttccgcacc ggtggtgaag aacgcggttt tggcctgccc gctcaccggg gcaagggcgt
    27241 tgattttctc cgccagggtg acgtagcttt catatggcac aatctgatac gcggtgtggg
    27301 taaactgttg cagctgctgc tccaccgccg cgaccagatc agggtggcga tgtccggtat
    27361 tcagcaccgc aatgcctgcg gcgaaatcga tgtactcgtt gccctcaaca tccttcagcg
    27421 tggcgttttc agccgactgg gcgaagaagt tacacatcac gccaaccccg cgcggggtgg
    27481 cagaaagacg acgctgatgg aattcattgt tgctcatgtt tcacaccttt tgactcttca
    27541 ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc tgtaatacgc cgcgcccatc
    27601 gcggtaaacg gtacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg
    27661 aaggcatcga aacgttcggc gtcgccgcgc agcagttcgg caatcagacg cccggcgagg
    27721 tgggtacagg tgacgccgtg gccgctgtag ccctgcatgt aatagatgtt ggtatcgagg
    27781 cgaccaaact gcggcatacg cgacagggtc agcaggaagt tgcccgtcca gcggtaatca
    27841 attttcacgc ccttcagctg cgggaaggtt ttcagcattt tcggcaccac aaggcgctca
    27901 acatcgtccg ggtcgcgagc gccatacact acgccgccgc cgtacagcag gcggttatcg
    27961 gcggtaagac ggtagtaatc aagcagatag ttacagtctt cgacgcagta gtttttcggg
    28021 ataagggaac gggctaaatc ttccgacagc cgctcggtgg tgatcacctg cgtgccgcac
    28081 ggcatgctgc gtttcgccag ctccggctcc actttatcgc ccaggtacgc attcccggcg
    28141 acaatcacat acttcgccgt cacctgacct gtggctgtac gcactacggc gggcgtggtg
    28201 tgctgaatct gcgtcactgc ggaaagctca tacacgcgcc cgccgttcag gcggatagcg
    28261 tccgcttcgc caatggcgag gttcaacggg tgaatatgtc cgccgctgtg atccaacagc
    28321 gcgccggtgt agcggtcgct ggccacttca cggcgaatgg cgttggcgtc cagcagttcc
    28381 agttgtttat tgccgtagcg ttcccagttc tctttctgct cttcgagtgt ggcgagttgt
    28441 ttatcattca tcgccacaaa cagaccgccg gggcggtagt cgcagtcaat ctgataacgt
    28501 ttgatacgtt cgcggatgat ctcaccacct tcgaacatca tgctgccgag catacgggcg
    28561 gtgtccatgc cgtagctttt ttcgatcaca tcgatgtcgc ggctgtagga gttcacaagc
    28621 tgcccgccgt tgcgtccgct ggcgccaaag ccgatgcgtg aggcttcgag aaccactacg
    28681 tcaaagcccg cttctgccag atgcagtgcg gaggaaagcc cggtatagcc gccgccgacc
    28741 acgcaaacgt cgcaggaaat cgactcattc agcgtgtcga atggcgcgta tttattcgca
    28801 ctggcggcgt agtaactgct ggtatgttcg gtcatttcag gcctccaggc ttatccagat
    28861 ggttttcagt tcggtgaatt tttcaagggc atgcagggat ttgtcgcgac cgttgccgct
    28921 ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat tgacgaagac
    28981 ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc gcgtccatac
    29041 cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgt agcgcctgtt cttctgatgt
    29101 gaaacgcgtg accaccagca ccggaccgaa aatctcttcg cggcttaagg acgcattcgg
    29161 atctacatcc acaaagatgg tcgggccgat ggcggcagcc agcccggcgt tacggccatc
    29221 caacaacagt tgccctttgc tttcgccttc ccgaataaag ctatggaccg agtcggcgtg
    29281 ggcgcagtcg attaaggtgc ccatggtggt tgcgggatca agtggatggc ccggctgcca
    29341 gttttgcgcc tgctgtttta acagggctaa gaattcatcg gcgatgctct cttccagcaa
    29401 caggcgcgtt ccggcgatgc acacctgacc ctggttgtag aagatgcctg ctgcggtggc
    29461 gcttgccgcc tgttgcaaat ccgggcagtc agcgaaaacg atgttggcgc ttttgccgcc
    29521 cgcttccagc cagacgcgtt tcatgttgct gtcgcccgca tctttcagca gctgtttccc
    29581 ggtacgggtt gaaccggtaa aggcgatggc atcgatatcg ttatgacgcg aaagcgcctg
    29641 ccccgcttca tgaccaaaac ccgttactac gttcagtacg ccatccggca gacctgcttc
    29701 tttcgccagc ccggcgagac gaatcgcact gagcggtgat ttttcagacg gttttagaat
    29761 cacgctgttt cccgccgcca gcgccgggcc gagtttccag caagtcagca gcagcgggaa
    29821 gttccacggc acgatggcgg caattacgcc gaccggttca cgcacgatca tcgccagctc
    29881 atggctactg gtggtcgcca cttcgccata cactttgtcg atcgcttcgg cgtaccagcg
    29941 aatggcgcgc gccgcgccgg gaatatcatc acgcagactg tgacgaatcg gtttgccggt
    30001 gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct tccattaaat cggcgagttt
    30061 attcagtaca gctttacgtt tagccggaga agagagtgac cagtcgccgc gttcaaatac
    30121 gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg caattttggc
    30181 cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt
    30241 atattcaccg ttaataaata agcgggtttc aatggcgaga cttaacgctt tatcctgcca
    30301 gtaagccaga tgatgaaaat tcattatgac tcctgtttca cgtctatcag atatatgcag
    30361 tttgtgaacg tcggggcgca gagatcccca ggccgtatgc ccgtttattt ctggcgacat
    30421 tctttatgga ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac
    30481 gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg
    30541 tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta atcctgacca
    30601 ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc ctgatgctta
    30661 attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga
    30721 ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag
    30781 tcgtcctgat taatcacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc
    30841 gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact
    30901 ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga
    30961 cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc
    31021 tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga
    31081 tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc
    31141 tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa
    31201 ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct
    31261 tcgccccttg cccatgcaga gagtttaccc aaaagttgct acattcaggt aacaacgcag
    31321 acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg
    31381 gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca
    31441 acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg aaaatgggga
    31501 tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc ccgggatcgg
    31561 cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactaccag
    31621 gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taatgacggt tccgccagcg
    31681 catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt
    31741 gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca ccgataaccg
    31801 gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta tcgccattac
    31861 tgcggtttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg ttttcatttt
    31921 tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg aatggtggtc
    31981 attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa
    32041 ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct
    32101 ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt
    32161 tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga
    32221 agttggagaa agggtgttat ttcccggcct cggtgtttgc aatggatatt ctgggcaacg
    32281 tagtagaaga agcgggtctg ggtcaggaaa tgggcgagcc ggatcgtact tgtgttcctg
    32341 ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag atgctcctga
    32401 ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc
    32461 tgtggcagca gctacgccag cgcggattgt tcccggttgt agcggtagag ctggagttct
    32521 atttactgga tcgtcagcgc gacgctgaag ggtatctgca accgccctgc gcgcccggca
    32581 ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac cacttcgccg
    32641 atgtgctcaa tgatattgat gaactggcgc agttacagtt aattcccgcc gacggtgcgg
    32701 tcgccgaagc ctcgccgggt cagtttgaaa tcaaccttta ccataccgat aacgtgctgg
    32761 aagcctgcga tgatgcgctg gcgctaaaac gcctggtgcg tctgatggca gaaaagcata
    32821 agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc ggaatgcata
    32881 tccatatcag tatgcaaaat aaccgtggcg agaacgtgct ttctgacgcg gaaggcgaag
    32941 attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg tcgtcgatgg
    33001 cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg tatgtgccga
    33061 cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg tgcggcgacc
    33121 gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca tatctggtga
    33181 tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg ttgcaggaag
    33241 aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc cagagcgatg
    33301 ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa cggttttgcc
    33361 atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc atcacagaaa
    33421 cagaaattga gtggatgttg aaaaacgcgt aatacccctt gcggggttgt ttcatcggcc
    33481 tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat gtcgcgttat
    33541 ggagcgcggg cggcaacggg cttccgttaa cgattaagac tgtgacgagg aaatgagatg
    33601 atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc tcctgcgact
    33661 gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag gtcgacacga
    33721 ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac cactgaatat
    33781 tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggtggtaat
    33841 gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta ttgtctccgg
    33901 cattagcgac ggtcacgttc cagcgtccta tttgctggcg ctggcgggcg tgctgtttac
    33961 cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta
    34021 cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc ggctggtcat cactgctgga
    34081 ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct ccgccctctt
    34141 cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa ccgccgcgaa
    34201 tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc
    34261 catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag aaggcgttgg
    34321 caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc cgattattac
    34381 cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca cgctttcgga
    34441 agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtatacgg
    34501 cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg atatcagccg
    34561 ctttaaagac ccggacgccg cactgcctga aattgcgctg tacgtcggcg gcaagctctt
    34621 tcagtcgatt ttcctctgca ccacgtttgt gaacacatta gcgtctggcc tggcttcaca
    34681 tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc cggagcgcgt
    34741 gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta tggtcgggat
    34801 tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg acagcactga ttaacttcgg
    34861 tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcggcgtaa
    34921 aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc tggttggcgc
    34981 gctgacggtg ggtgtgctgt gggttaacct agagtcaacg tcactgacac tcggtctggt
    35041 atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc gcaaagtgcc
    35101 gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg cgcggcgcat
    35161 tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat gacccggatc
    35221 acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa ataatgaacc
    35281 acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa tgcgcagagc
    35341 ctgtcgcaga ggctgaacgc acaccgctgg cccactactt ccagttactg ctcacccgtt
    35401 tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct gaagcgggga
    35461 ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt aatgagtaat
    35521 tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca cgccgcatcc
    35581 ggctaacaat gacacgcact gcaacgtgct ttaatgggtt tctcacgatc gtaccgctct
    35641 gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt acgaatgttg
    35701 aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta cgacccctga
    35761 acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt tacataaaat
    35821 cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg
    35881 gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg
    35941 tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa
    36001 ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct
    36061 atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc
    36121 gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact cccactcgta
    36181 aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc aacaacccgt
    36241 ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt gccgataacg
    36301 tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc cagccggatg
    36361 cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc
    36421 ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcaccggtc
    36481 cgtatcaatt gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact
    36541 tctggcgcgg taaaccgtta atgccgcagg tggtggtgga tttaggctcc ggcggcaccg
    36601 gacgtctgtc gaaactcctg actggtgaat gcgacgttct ggcctggcct gctgccagcc
    36661 agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtccc gggatgaacg
    36721 tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg
    36781 cgctggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg
    36841 aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta
    36901 ctgaatacga tccggcgaaa tcgcgcgaac agttgaagtc gttggggctg gaaaatttaa
    36961 cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg
    37021 ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag
    37081 aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg ttatccggtt
    37141 gggcgacgga cagtaacgac ccggacagtt tcttccgtcc tttactgagc tgcgcggcaa
    37201 ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc gtgttgcgta
    37261 aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa gcgcagagta
    37321 ttctggcgca ggaattgccc attttgccgc tggcgtcgtc attgcgtttg caggcctatc
    37381 ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt
    37441 atcgcgagaa acaggatgag gtgaaaaagc catgattatc ttcaccttac gccgcatttt
    37501 gttattgatt gttaccttgt tcctgctgac cttcgttggc ttcagtttga gctatttcac
    37561 tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaatgg
    37621 cctgatccac tgggattttg gtgtttccag cattaacggt caaccaattg ccgaacagtt
    37681 aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt ttgcgctgat
    37741 tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt
    37801 gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt
    37861 gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct
    37921 ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc
    37981 atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct
    38041 gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta tcgaagtgta
    38101 tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg
    38161 tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc
    38221 caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg
    38281 ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt
    38341 gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa
    38401 ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc
    38461 cgggcacgct gcgtacagcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc
    38521 tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt gcgccttacg
    38581 gcatcgacca gcaatttctc ggttatcaat tattgccgcc gtcatggtcg cgctatggcg
    38641 aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc cgcttactga
    38701 gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg acgatctgtg
    38761 gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg gtgcttaacc
    38821 atattctgga taccttactg gcgatccctt cgctgctgtt ggcaattatc gttgtagcgt
    38881 ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg ctgccgcgta
    38941 tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg
    39001 cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg ccaaacatca
    39061 ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg
    39121 cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg
    39181 gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa
    39241 ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt gcgattattg
    39301 cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat ttaaaaccgg
    39361 tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag gtgaaatccg
    39421 cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga tttgtggggt
    39481 gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata tcgatttgct
    39541 gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga tgattttcca
    39601 ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga tgcaaaacat
    39661 cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc
    39721 cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc gcagttttcc
    39781 ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca
    39841 accgcgtctg ctgattgctg acgaaccgac caactcaatg gagccaacaa cccaggcgca
    39901 aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt tgcttatcag
    39961 ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca
    40021 aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc cttataccca
    40081 ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa gtcgcctcaa
    40141 tacgctgccc ggcgctatcc cactgctgga acagttaccg attggttgtc gtctggggcc
    40201 acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa
    40261 tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc
    40321 tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggctgg tttcgtcgtc
    40381 agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga
    40441 ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg ggaatgatag
    40501 agcctaccag cggcgagtta ttgattgacg atcatccact gcattttggc gattattcct
    40561 tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc
    40621 aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccgacctg gaaccggaac
    40681 agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca
    40741 gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg gcgcgcgcgc
    40801 tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg gatatgtcga
    40861 tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc atttcgtata
    40921 tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc
    40981 atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg
    41041 aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacggcg gatgcgtggc
    41101 gtaaagatcg ctaattttct gaatcctcct tccagattat ccacctcatt ctgacaagat
    41161 ttaatctctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg tgatttaaga
    41221 cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg acgttttggt
    41281 gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt tgcttgctgc
    41341 tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt tgcctatgat
    41401 accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt ctggcataac
    41461 gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact gaaccgccga
    41521 tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt gtggtgaaaa
    41581 catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc atcgccataa
    41641 aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatatgg
    41701 tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgcccac cctgccgcag
    41761 cctgctccgg tcggacctgg caactatagc tactcacagc caggttgatt ataataaccg
    41821 tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc
    41881 atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg
    41941 tgttgccagc aaactatcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc
    42001 tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc
    42061 tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac
    42121 catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg
    42181 ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaggctt
    42241 caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc
    42301 catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat
    42361 cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat
    42421 ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg
    42481 tactctggcg gcttccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt
    42541 taccccgatt cgccgtaccg ttactattga agatgtgggt aactctgcgg cattcctgtg
    42601 ctccgatctc tctgccggta tctccggtga agtagtccac gttgacggcg gtttcagcat
    42661 cgctgcaatg aacgaactcg aactgaaata atcgttctgt tagtaaagat gggcggcgtt
    42721 ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct
    42781 ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa
    42841 caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactgcggag
    42901 tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac
    42961 gtgatccttg atgaagaaac gctggctccc ttcctttcat ggctggtccc tgcgcgcgtt
    43021 cttgcagtgg aattgttccc tgaccagctt accgtgaccc gttcacagac cttcaccgct
    43081 tatgaacgct tgtctacggc cctgacggtt gctcaggttt gcggcgtcca gcggttatgt
    43141 aactactatt cggcgcgact tacgccgctc cccgggcctg attcctccag ggaaagcaat
    43201 cataggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc
    43261 gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgcctggga ctgtgtgatc
    43321 attaaccaaa tcattggttt tattggcttt caggcgcgga ccattgcgac atttcaggct
    43381 tatctcgggc acccggtacg ctggttaccc ggtctggaaa tacaaaacta cgccgacgcg
    43441 tcactgtttg ctgatgaatc aatacgctgg cgaagcagct atgaggtgga aaaactacct
    43501 gaagagtaca caaaaagttc aacagcagaa ctttgccaac tggccgaaac actctctctc
    43561 caccctattt cactttccct tctcgaaagg ttgttaaaca gtacacgggt taatacacag
    43621 ccggataata agcttgcggc gttgttatgc gcgcggataa atggcagtcc tgcttgtttt
    43681 gccgcctgta tggattcatc aaatgaatat aaaaaaatca gcccccttct gcgcaagggc
    43741 gaaaatgaaa ttaaccaatg ggctgaccgt cattctgttg agcacgctac cgttcaggcg
    43801 atacaatggc tgacccgagc acccgatcgc tttagcgccg cccagttcag ccctttactc
    43861 gaacacgaaa aatcatcaac gcagattatt aatctgctgg tatggagcgg gctgtgtggc
    43921 tggataaatc gcttaaaaat cgcgttgggt gagacatatt aaccttgccg cgtcagacag
    43981 attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa tagacaatta tgtttcagga
    44041 caacccgctg ctagcgcagc ttaaacagca actgcattcc cagacgccac gcgctgaagg
    44101 ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta
    44161 tttcattccg ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat
    44221 ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa ctggttgaac cgttcctgac
    44281 tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg gccatcgttc ctgatcatcc
    44341 actcttaaaa gacgccattc cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga
    44401 aggcgactgg gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc gttctttcta
    44461 tgctgaactg acacaataca tcacttttgg tgacgaccac tttgtaccgt ggtgggttac
    44521 ccttgcgcgc cataatctgg aaaaagaagc accagacggc gtcgctaccg aaatgctcga
    44581 tgaaggtctg gttcgtgaag atctgaccgc gctggatttt gtcaccatcg acagtgccag
    44641 cacagaagat atggatgacg cccttttcgc taaggcgttg ccggatgaca aacttcagct
    44701 gattgtggcg attgccgatc caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc
    44761 cgcgaaaatt cgcgcattca ccaactatct gcctggcttc aacatcccta tgctgcctcg
    44821 cgagctttct gacgatctct gctcactgcg cgccaatgaa gtccgcccgg tactggcatg
    44881 ccgcatgacg ctctccgctg atggcaccat tgaagataat atcgaattct ttgccgccac
    44941 catcgaatcc aaagcaaagc tggtgtatga ccaggtttct gactggctgg aaaataccgg
    45001 tgactggcag cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg
    45061 ccaacgccgc ggcgagtggc gtcataacca cgcactggtg tttaaagatc gcccggatta
    45121 ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat
    45181 tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg cccgcgtact
    45241 gcgcgataag ctcggttttg gcatctataa cgtgcatatg ggctttgatc cggcgaatgc
    45301 cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct
    45361 cacgctggac ggtttctgca aactgcgtcg tgaactggac gcgcaaccaa ctggtttcct
    45421 cgacagccgc attcgtcgct tccagtcatt tgctgaaatt agcactgaac ccggtcctca
    45481 ctttggcctc ggtctggaag catacgccac ctggacttcg ccgatccgta aatatggcga
    45541 catgatcaac caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga cgcgcccaca
    45601 ggatgaaatc actgtccaaa tggccgagcg tcgccgtctc aaccggatgg cagaacgtga
    45661 tgttggtgac tggttatacg cacgcttcct gaaagacaaa gccgggaccg acacccgttt
    45721 cgcagcggaa attgtcgata tcagccgtgg cggcatgcgt gttcgtttgg ttgataacgg
    45781 cgctatcgcc tttattccgg cacctttctt acacgctgtg cgcgatgaac tggtttgcag
    45841 ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt tacaaagtga ctgacgttat
    45901 tgacgtcact attgccgaag tccgcatgga aacccgcagc attattgcgc gcccggtcgc
    45961 gtaatctcct ttcacggccc attcctcatg gatgggccgt ttatttcccc gctctacctt
    46021 cgttatatcc aggcagattt tttaactgac tttcgtttga aaactggcgt ttttccaaaa
    46081 ctggattaca aataaatata taaaaaaaac aatgcgttta gccgccgtta atttcatgta
    46141 agtcatgttg ttccttttat ttaaccgcgg actccgcagt taaccggagg atatgcatca
    46201 tgaaaaccgt tagggagtcc acaacgttgt acaactttct cggatcgcac aatccatact
    46261 ggcggttgac ggaaagcagc gatgttttgc gcttttctac caccgaaacc acagaacctg
    46321 atcgtacttt gcagttatct gccgaacagg ctgctcgcat cagggaaatg acggtcatca
    46381 cctccagcct gatgatgagt ctgaccgtcg atgagagcga tctttctgtg catctggtag
    46441 gacgaaaaat caataaacgg gaatgggctg gcaacgcgtc tgcatggcat gacacaccgg
    46501 cggttgctcg tgatttatca cacgggcttt cctttgctga gcaggtagtt tctgaagcac
    46561 attccgcaat agtcattctc gacagccggg ggaatattca acgcttcaat cggttatgtg
    46621 aagattacac agggttgaaa gaacacgacg tcattgggca aagcgtgttt aaactgttta
    46681 tgagccgtcg tgaagctgcg gcatccaggc gcaataaccg tgtatttttt cgaagcggca
    46741 atgcatatga agtcgaactg tggataccaa catgtaaagg ccagcggctg tttctgtttc
    46801 gcaataaatt tgtccacagc ggcagtggca aaaacgagat ttttttaatc tgttccggca
    46861 ccgacattac cgaagagcgc cgcgctcagg agcgactgcg tattctggca aataccgaca
    46921 gtatcaccgg actgccgaat cgtaacgcaa tgcaggattt aatcgatcac gctattaatc
    46981 atgcagataa caataaagtt ggggttgtgt atcttgattt ggataatttc aaaaaggtca
    47041 acgacgccta tgggcatttg tttggtgacc agttattacg cgacgtgtca ttggctattt
    47101 taagctgtct cgaacatgac caggtgttgg cgcgtccagg tggggatgag tttctggtac
    47161 tggcatccaa cacctcacaa agcgcgctgg aagcaatggc atcacgaatt ttgacccgct
    47221 tacggctccc ctttcgcatt ggtttaattg aagtttatac cagctgttca gtaggtattg
    47281 cactctctcc cgaacatggt tcagacagca cggctattat tcgtcacgcc gacacagcaa
    47341 tgtacacagc gaaggaaggc ggacgaggac aattttgcgt ttttacccca gaaatgaatc
    47401 aacgggtatt tgaatatctc tggctggata ccaacttgcg taaagcactg gaaaacgatc
    47461 agttggttat tcactatcaa ccgaaaatca cctggcgtgg cgaagtgcgc agtctggaag
    47521 cactagtacg ttggcagtca cctgaacgtg ggttgattcc accgttggac ttcatttcct
    47581 acgccgaaga gtcagggcta attgtgcctt taggccgttg ggtgattctc gatgtcgtac
    47641 gccaggtggc aaagtggcgg gataaaggca taaacctgcg agtggcggta aatatttctg
    47701 cacgtcagct cgccgatcaa accattttca ccgccctgaa acaggttctc caggaactca
    47761 attttgaata ctgccctata gatgttgaac tgacagagag ttgtctgatt gagaatgatg
    47821 aactggcact gtctgttatt caacaattta gccaactagg tgcgcaagtg catctggatg
    47881 attttggtac cggctactct tcactttcgc aactggcgcg ctttccgatc gatgccatca
    47941 aacttgacca ggtttttgtt cgagatattc acaaacaacc tgtctcgcag tcactggtcc
    48001 gggcgatcgt cgctgtggcc caggcattga atcttcaggt gatcgccgaa ggtgtagaga
    48061 gtgcaaagga agatgctttt ttaaccaaga acgggatcaa tgagcggcaa ggatttttgt
    48121 ttgccaaacc gatgcccgcc gtcgccttcg aacgctggta taaacgctat ctgaagcgcg
    48181 cataagtaga tgaatatata tacttagcgc tatatcccgt cattatatat cgtattcctg
    48241 gatgataatt agccgttgct gttaaacact tctcttttag cggcaatttt tgcattattc
    48301 attttccact tttgctataa ggatgtcctt gatgtctgaa tttgatgccc agcgcgttgc
    48361 cgaacgtatt gatattgtgt tagatatcct tgtggcaggc gattaccact ctgcgatcca
    48421 taatctcgag atacttaaag cagaactctt gcgccaggtt gctgaatcta cgccagacat
    48481 cccgaaggca ccgtgggaga tttaacaatc gtgccttcag gttttcctat ttgaggatgc
    48541 ccggcgcgtc agtatttttt tatttagtat tataacgtta taagaattac agcgatgaat
    48601 tcccgacaac aaactattct acagatggtc attgaccagg gtcaggttag cgtaaccgat
    48661 ctggcaaaag ccactggagt ttctgaagtt accattcgcc aggatctcaa caccctcgaa
    48721 aaactgagtt acctccgccg tgcacatggc tttgcagttt cgcttgatag tgatgacgtc
    48781 gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac tcgccgagtt tgccgcgtca
    48841 ctggttcaac cgggcgaaac catctttatc gaaaatggta gcagcaatgc cctgctcgcc
    48901 cggactctgg gggagcagaa gaaaaatgtc actatcatca cggtcagcag ctacatcgcg
    48961 catttgctga aagacgcgcc ttgtgaagtc attttgctcg gtggcgtgta ccagaaaaaa
    49021 agcgaaagta tggttggccc gttgacacgc cagtgtatcc agcaggtgca tttcagcaaa
    49081 gcatttattg gtattgatgg ctggcaacct gaaactggat tcaccggtcg cgatatgatg
    49141 cgtaccgatg tggtcaatgc cgtgctggaa aaagagtgcg aagcgatagt cctgactgac
    49201 agctcgaaat ttggtgctgt acattcatac tccatcggtc ccgttgagcg attcaatcgc
    49261 gtgattaccg attcgaaaat acgcgccagc gatctgatgc atcttgagca cagtaaactc
    49321 accgttcacg tcgttgacat ttaaccattt cccttctcta cggatgattt gcagtttggc
    49381 aaatcatccg ctctaagatg attcctggtt gataattaag actatttacc tgttattaac
    49441 acgcttaaga tataaaatta ttatcagcga tataacagga agtcattatc acctgcgtga
    49501 tataaccctg cgcgcgagca g
//
LOCUS       DAPHJE010000018        49442 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPHJE010000018 DAPHJE010000000
VERSION     DAPHJE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.018"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..619)
                     /locus_tag="RH222_001071"
     CDS             complement(<1..619)
                     /locus_tag="RH222_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV3796125.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCK"
     gene            complement(742..1194)
                     /gene="tabA"
                     /locus_tag="RH222_001072"
     CDS             complement(742..1194)
                     /gene="tabA"
                     /locus_tag="RH222_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV3796126.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1339..1932)
                     /locus_tag="RH222_001073"
     CDS             complement(1339..1932)
                     /locus_tag="RH222_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV3796127.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2003..2716
                     /gene="bdcA"
                     /locus_tag="RH222_001074"
     CDS             2003..2716
                     /gene="bdcA"
                     /locus_tag="RH222_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3796128.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            2847..3242
                     /locus_tag="RH222_001075"
     CDS             2847..3242
                     /locus_tag="RH222_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV3796129.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            3523..3657
                     /gene="pyrL"
                     /locus_tag="RH222_001076"
     CDS             3523..3657
                     /gene="pyrL"
                     /locus_tag="RH222_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV3796130.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            3661..4596
                     /gene="pyrB"
                     /locus_tag="RH222_001077"
     CDS             3661..4596
                     /gene="pyrB"
                     /locus_tag="RH222_001077"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV3796131.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4609..5070
                     /gene="pyrI"
                     /locus_tag="RH222_001078"
     CDS             4609..5070
                     /gene="pyrI"
                     /locus_tag="RH222_001078"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV3796132.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5143..5529
                     /gene="ridA"
                     /locus_tag="RH222_001079"
     CDS             5143..5529
                     /gene="ridA"
                     /locus_tag="RH222_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV3796133.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNAAF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(5735..8431)
                     /gene="mgtA"
                     /locus_tag="RH222_001080"
     CDS             complement(5735..8431)
                     /gene="mgtA"
                     /locus_tag="RH222_001080"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV3796134.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(8572..8625)
                     /gene="mgtL"
                     /locus_tag="RH222_001081"
     CDS             complement(8572..8625)
                     /gene="mgtL"
                     /locus_tag="RH222_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV3796135.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8810..9757
                     /gene="treR"
                     /locus_tag="RH222_001082"
     CDS             8810..9757
                     /gene="treR"
                     /locus_tag="RH222_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV3796136.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPTTLS"
     gene            9876..11297
                     /gene="treB"
                     /locus_tag="RH222_001083"
     CDS             9876..11297
                     /gene="treB"
                     /locus_tag="RH222_001083"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV3796137.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11347..13002
                     /gene="treC"
                     /locus_tag="RH222_001084"
     CDS             11347..13002
                     /gene="treC"
                     /locus_tag="RH222_001084"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV3796138.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWHDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NNGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALT
                     DESSVFYTYQKLIALRKQEAVLTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRG
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13396..15534
                     /gene="nrdD"
                     /locus_tag="RH222_001085"
     CDS             13396..15534
                     /gene="nrdD"
                     /locus_tag="RH222_001085"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV3796139.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            15692..16156
                     /gene="nrdG"
                     /locus_tag="RH222_001086"
     CDS             15692..16156
                     /gene="nrdG"
                     /locus_tag="RH222_001086"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV3796140.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16201..16587)
                     /gene="cybC"
                     /locus_tag="RH222_001087"
     CDS             complement(16201..16587)
                     /gene="cybC"
                     /locus_tag="RH222_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV3796141.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(16770..18122)
                     /gene="pmbA"
                     /locus_tag="RH222_001088"
     CDS             complement(16770..18122)
                     /gene="pmbA"
                     /locus_tag="RH222_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV3796142.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18216..18767
                     /locus_tag="RH222_001089"
     CDS             18216..18767
                     /locus_tag="RH222_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV3796143.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(18918..20291)
                     /gene="mpl"
                     /locus_tag="RH222_001090"
     CDS             complement(18918..20291)
                     /gene="mpl"
                     /locus_tag="RH222_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV3796144.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20467..21465
                     /gene="fbp"
                     /locus_tag="RH222_001091"
     CDS             20467..21465
                     /gene="fbp"
                     /locus_tag="RH222_001091"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV3796145.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21498..22493)
                     /gene="yjfF"
                     /locus_tag="RH222_001092"
     CDS             complement(21498..22493)
                     /gene="yjfF"
                     /locus_tag="RH222_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV3796146.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22480..23502)
                     /locus_tag="RH222_001093"
     CDS             complement(22480..23502)
                     /locus_tag="RH222_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3796147.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(23516..25018)
                     /locus_tag="RH222_001094"
     CDS             complement(23516..25018)
                     /locus_tag="RH222_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3796148.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25328..26284)
                     /gene="ytfQ"
                     /locus_tag="RH222_001095"
     CDS             complement(25328..26284)
                     /gene="ytfQ"
                     /locus_tag="RH222_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV3796149.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26594..27124
                     /gene="ppa"
                     /locus_tag="RH222_001096"
     CDS             26594..27124
                     /gene="ppa"
                     /locus_tag="RH222_001096"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV3796150.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27204..27554)
                     /gene="chpB"
                     /locus_tag="RH222_001097"
     CDS             complement(27204..27554)
                     /gene="chpB"
                     /locus_tag="RH222_001097"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDV3796151.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(27548..27799)
                     /gene="chpS"
                     /locus_tag="RH222_001098"
     CDS             complement(27548..27799)
                     /gene="chpS"
                     /locus_tag="RH222_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDV3796152.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(28012..28353)
                     /locus_tag="RH222_001099"
     CDS             complement(28012..28353)
                     /locus_tag="RH222_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV3796153.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28356..32135)
                     /gene="tamB"
                     /locus_tag="RH222_001100"
     CDS             complement(28356..32135)
                     /gene="tamB"
                     /locus_tag="RH222_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV3796154.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32132..33865)
                     /gene="tamA"
                     /locus_tag="RH222_001101"
     CDS             complement(32132..33865)
                     /gene="tamA"
                     /locus_tag="RH222_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV3796155.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34071..34709
                     /gene="msrA"
                     /locus_tag="RH222_001102"
     CDS             34071..34709
                     /gene="msrA"
                     /locus_tag="RH222_001102"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV3796156.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35032..36375
                     /locus_tag="RH222_001103"
     CDS             35032..36375
                     /locus_tag="RH222_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV3796157.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36437..36643)
                     /locus_tag="RH222_001104"
     CDS             complement(36437..36643)
                     /locus_tag="RH222_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV3796158.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36968..37525
                     /locus_tag="RH222_001105"
     CDS             36968..37525
                     /locus_tag="RH222_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV3796159.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(37515..38255)
                     /gene="cysQ"
                     /locus_tag="RH222_001106"
     CDS             complement(37515..38255)
                     /gene="cysQ"
                     /locus_tag="RH222_001106"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV3796160.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38445..40388
                     /gene="cpdB"
                     /locus_tag="RH222_001107"
     CDS             38445..40388
                     /gene="cpdB"
                     /locus_tag="RH222_001107"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV3796161.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(40517..40897)
                     /locus_tag="RH222_001108"
     CDS             complement(40517..40897)
                     /locus_tag="RH222_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV3796162.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40986..41846
                     /gene="qorB"
                     /locus_tag="RH222_001109"
     CDS             40986..41846
                     /gene="qorB"
                     /locus_tag="RH222_001109"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV3796163.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41954..42919
                     /locus_tag="RH222_001110"
     CDS             41954..42919
                     /locus_tag="RH222_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3796164.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43027..43689
                     /gene="ytfE"
                     /locus_tag="RH222_001111"
     CDS             43027..43689
                     /gene="ytfE"
                     /locus_tag="RH222_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV3796165.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLSRAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43936..45003)
                     /locus_tag="RH222_001112"
     CDS             complement(43936..45003)
                     /locus_tag="RH222_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796166.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(45102..45536)
                     /locus_tag="RH222_001113"
     CDS             complement(45102..45536)
                     /locus_tag="RH222_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796167.1"
                     /translation="MAKHSWLLIAALPLSISPSWGADFYYRQQEKGTVYVAEQKGEKD
                     EILSELPGVNFSRLWRIANLANNQETRLLSDFNPDKFDCDDRNNCQHTWLTDGRSVLW
                     SGKVLKNPPGEPNVDAASFQAFGAFAADKRSVYFDGQRTDDR"
     gene            complement(45803..47206)
                     /gene="cycA"
                     /locus_tag="RH222_001114"
                     /gene_synonym="dagA"
     CDS             complement(45803..47206)
                     /gene="cycA"
                     /locus_tag="RH222_001114"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV3796168.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(47515..48135)
                     /gene="fklB"
                     /locus_tag="RH222_001115"
     CDS             complement(47515..48135)
                     /gene="fklB"
                     /locus_tag="RH222_001115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3796169.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            48354..48992
                     /gene="ytfB"
                     /locus_tag="RH222_001116"
     CDS             48354..48992
                     /gene="ytfB"
                     /locus_tag="RH222_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV3796170.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(48976..>49442)
                     /locus_tag="RH222_001117"
     CDS             complement(48976..>49442)
                     /locus_tag="RH222_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV3796171.1"
                     /translation="LEYTWNNIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANPWFL
                     KIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNINIA
                     ALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
BASE COUNT        12094 a        13251 c        12276 g        11821 t
ORIGIN      
        1 ctttacaact cacctgcgag ttattcaagt cctgagaatt atttaactcc tgcggaatat
       61 ttaatagacg agctatttcc ttgttagctt cgcgttggta acacgccaga atcttgttag
      121 taatcaggtg taatgaactc tcatcttttc tttcaaagga cttcccttca taacgattag
      181 tcgaaccatc aggttgtgta aatataatga ctgtatgtcc ttcacgaatc ccattattaa
      241 aagctgaggc aagtgtaatt tctatttcca ccttagataa atccgctcgc tccccttcag
      301 gattattcat aacaaacttc attatctcac taccatcatt atttttgata taagatgagg
      361 acttaatatg agccatcata tttctacccg cttcgttgga gcgtttgcta aaatccacat
      421 aacccagggt aaatatctta gccaaaaact ccaaaaatgc tctgggagaa agaatattgc
      481 ttctgctatt ctgccctccg gcaattcggg caaaatcatt cattcccaca taccaggtat
      541 ctaagtcttc atttttttgt ttacaattgt cggctgatgt aatgttttga gaaaaattcc
      601 aacctgatat tttgctcatg acggactcct tgagttaata agaacgtcta agcattacaa
      661 atgctactca caaaaaaata tcaaaaaacg ctcttttcac aaactcgccg taaacctgga
      721 gttatttacg gcgaaaagtc atcacgccat cagcatcttc actactgctt tgcgaacctg
      781 ggctggcgcg cccactgcgc acagcggttt atgcacttcc cccggataaa acacaacaaa
      841 atcaccttca ttaaggataa ctgttttctc atcaacgcct tccggcaaaa atgcgatgtc
      901 tttatcagct aaccagtcgg tatccggcgt gcctgcaggt tgcgtgctga aggtcatgcc
      961 ttcctgacct tttaacacaa tctgaatgtc gagatagcgg gcatgatact ccgcacggcg
     1021 agcttcgtac ggctcggtca tatcttccga gataagataa aacagacgat tgccttcgat
     1081 atcgtgctta ccctttggcg tttctgccgt aacgtgcgct ttgatatgct caatcgcctg
     1141 gcgtaactcc tggggtagcc acggctgaag attatgaata tttccgatga tcatcttttc
     1201 cactccttaa ttaaaacaat gtttcatttc tatcgttata cgaaaagatg atcaattccg
     1261 ccagccttaa cgcgtgaatt attgtcttat cacaacttat cggcaggcgc gttcccgcct
     1321 ttcggatcaa ggccagcatc actccttgag aagagttttt atcgcttccc ccgccagtgc
     1381 agctgttgca cagagttgtt cgattgagtg cccctcccgt gccttcgcag aaagccctga
     1441 catcacggta ctcataaaat cagtcacaca ttgtgcgctt tgtggatgcc gcctggcgat
     1501 atagtcataa atggtcgttt ctgcggcgtg ataatattga acggcaatat cacgcgctag
     1561 tggatcatga ctatgaatac cttcaagaac catacagcca gcgcagccgc cgttttggct
     1621 atatctgcgc gccgcttctt ttaatacctc aaccaggcac tcgcctactg gacgatcatc
     1681 acgaagaata tcggcaagcg gaatagcttc cgtaccgacg tattcattga gtacacggct
     1741 aaataaccca gctttactgc caaaagccgc gtagaggctc gggggattaa taccaagata
     1801 atcagtaact tcagcaacac tgacagcatc gaaacctttt tggtgaaaaa gcacttttgc
     1861 cgcagagact gcctgctcag gagcgaaacg tctgggacga cctggaacgc gagattgttt
     1921 tttagtgacc attacaaaac ttgttgacag aaagttaaaa cagttttgta atgcatgtta
     1981 cataataaat caaggagtcc ttatgggcgc ttttacaggt aagacagttc tcatcctcgg
     2041 tggcagtcgt ggtatcggtg ccgctatcgt acgtcgtttc gtcaccgatg gggccaatgt
     2101 acgattcacc tatgcggggt cgaaagatgc cgctaaacgc ctggcacaag agactggagc
     2161 gacagcagta ttcacagata gtgctgacag agacgctgtc attgatgtcg ttcgtaagag
     2221 cggcgcattg gatatcctgg tggtaaatgc aggtattggc gtctttggcg aggccctgga
     2281 attaaatgcc gacgatattg atcgcctttt caaaatcaat attcatgctc cttatcatgc
     2341 ctctgttgaa gccgcccggc agatgcccga aggcgggcgc atcttaatca tcggctccgt
     2401 gaatggcgat cgtatgcctg ttgcaggcat ggctgcttat gccgccagca aatctgccct
     2461 acaaggcatg gcgcgcgggc tggcccgtga tttcggaccg cgtgggatca ccatcaacgt
     2521 cgtccagcca gggccaattg ataccgacgc taatcccgcc aacgggccaa tgcgcgatat
     2581 gttgcatagt tttatggcta tcaaaagaca tgggcaaccg gaagaggtcg ctggtatggt
     2641 cgcatggtta gcagggccag aagccagttt tgttaccggc gcgatgcata ccattgatgg
     2701 cgcgtttggc gcataaccga ctacgctcaa ttaagcccag ccatttccca tgatgtctgg
     2761 gttttgttta ctcacgtcgt ccgctaaaag cggctcctgg taaatataaa tcttctgttc
     2821 atgtcgtaaa tcttgaggat aaccaaatgg tagaaagaac cgctgttttc cctgctggcc
     2881 gacattcact gtatgcagaa catcgttatt ctgcggctat tcgttccggc gatttgctgt
     2941 ttgtttccgg gcaagtagga agtcgagagg acggaacacc agaacccgat tttcagcaac
     3001 aagtcagact ggcatttgat aatttgcatg cgaccctggc agctgcggga tgcacttttg
     3061 acgatatcat tgatgttacg agctttcata ccgatccaga aaaacaattt gaagacatca
     3121 tgacggtgaa aaatgaaata tttagcgcgc caccttatcc aacctggacg gcggtgggcg
     3181 ttacatggct ggcaggcttt gattttgaaa ttaaagtgat agcgcgcatc cctgagctgt
     3241 aagcaatagt gttagccgtt cgttttcaca ctccgcccta taagtcggat gaatggaata
     3301 aaatgcatat ctgattgcgt gaaagtgaaa aagaaaaaag caggaaatgt ctgcaattat
     3361 tgataccgaa cgacagttcc cctccagaat cacagtaaat aaaaatgcat atattttgac
     3421 ttataattca aataaaccgt ttgcgctgac aaaatattgc atcaaatgct tgcaccgctt
     3481 ctgacgatga gtataatgcc ggacaatttg ccgggaggat gtatggttca gtgtgttcga
     3541 cattttgtct taccgcgtct gaaaaaagac gctggcctgc cgtttttctt cccgttgatc
     3601 acccattccc agcccctcaa tcgaggggct tttttttgcc caggcgtcag gagataaaag
     3661 atggctaatc cgctatatca gaaacatatc atttccataa acgaccttag tcgcgatgac
     3721 cttaatctgg tgctggcgac agcggcgaaa ctgaaagcaa acccgcaacc agagctgttg
     3781 aagcacaaag tcattgccag ctgtttcttc gaagcctcta cccgtacccg cctctctttc
     3841 gaaacttcca tgcaccgcct tggtgccagc gtggtgggct tctccgacag cgccaataca
     3901 tcactgggta aaaagggcga aacgctggcc gataccattt cggttatcag cacttacgtc
     3961 gatgcgatag tgatgcgtca tccgcaggaa ggtgcggcgc gcctggccac cgagttttcc
     4021 ggcaatgtac cggtactgaa tgccggtgat ggctccaacc aacatccgac gcaaaccttg
     4081 ctggacttat tcaccattca ggaaacccag gggcgtctgg acaatctcca cgtcgcaatg
     4141 gttggtgacc tgaaatatgg ccgcaccgtt cactccctga ctcaggcgtt agcgaagttc
     4201 gacggcaacc gtttttactt catcgcgccg gacgcgctgg caatgccgca atacattctg
     4261 gatatgcttg atgaaaaagg gatcgcatgg agtctgcaca gctctattga agaagtgatg
     4321 gcggaagttg acatcctgta catgacccgc gtgcaaaaag agcgtctgga cccgtccgag
     4381 tacgccaacg tgaaagcgca gtttgttctt cgcgccagcg atctccacaa cgccaaagcc
     4441 aatatgaaag tacttcatcc gctgccgcgt gttgatgaga ttgcgacgga tgttgataaa
     4501 acgccacacg cctggtactt ccagcaggca ggcaacggga ttttcgctcg ccaggcgtta
     4561 ctggcactgg ttctgaatcg cgatctggta ctgtaagggg aaatagagat gacacacgat
     4621 aataaattgc aggttgaagc tattaaacgc ggcacggtaa ttgaccatat ccccgcccag
     4681 atcggtttta agctgttgag tctgttcaag ctgaccgaaa cggatcagcg catcaccatc
     4741 ggtctgaacc tgccttctgg cgagatgggc cgcaaagatc tgatcaaaat cgaaaatacc
     4801 tttttgagtg aagaccaggt agatcaactg gcattgtatg cgccgcaagc cacggttaac
     4861 cgtatcgaca actatgaagt ggtgggtaaa tcgcgcccaa gtctgccgga gcgcatcgac
     4921 aatgtgctgg tctgcccgaa cagcaactgt atcagccatg ccgaaccggt ttcttccagc
     4981 tttgccgtgc gaaaacgcgc caatgacatc gcgctcaaat gcaaatactg tgaaaaagag
     5041 ttttcccata atgtggtgct ggccaattaa ttgcggttgg taataaaagt ctggctccct
     5101 ataatgagcc agacttttta ccgctgtaat aaaggagaaa tcatgagcaa aactatcgcg
     5161 acggaaaatg caccggcagc tatcggccct tacgtacagg gcgttgatct gggcaatatg
     5221 atcatcacct ccggtcagat cccggtaaat ccgaaaacgg gcgaagtacc ggcagacgtc
     5281 gctgcacagg cacgtcagtc gctggataac gtaaaagcga tcgtcgaagc cgctggtctg
     5341 aaagtgggcg acatcgttaa aactaccgtg tttgtaaaag atctgaacga cttcgcaacc
     5401 gtaaacgcca cttacgaagc cttcttcacc gaacacaacg ccgccttccc ggcacgttct
     5461 tgcgttgaag ttgcccgtct gccgaaagac gtgaaaattg agatcgaagc gatcgctgtt
     5521 cgtcgctaat cttgatggaa atccgggcta tcatgcccgg attaagtctg atgacaaaca
     5581 caaaatcgcc tgatgcgcta cgcttatcag gcctacgtga ttcctgcaat ttattgaatt
     5641 tgttggccgg ataaggcatt tacgccgcat ccggcatgaa caaaactcac tttgtctgca
     5701 attcgaatcc aggccaccat gcccagttga ttctttattg ccagccgtaa cgacggctat
     5761 aaaatccttt caccaactgg gttaatgtca tataccctgc cagaatcgca accagccacg
     5821 ggaaatagct taacggcagc gcctgcagtt gcagataact ggccagcggt gaaaacggca
     5881 atgcgatccc gacaatcatc acgatcacgg tcatgatcat taacggccac gatgcacagc
     5941 tctgaataaa cggcacacgg cgggtgcgga tcatatgcac aatcagcgtt tgcgacagta
     6001 agcccaccac aaaccatccc gactggaaca gcgtttgcgt ttccggcgtg ttggcatgga
     6061 atacccacca catcaggcaa aacgtcagaa tatcgaagat cgagctgatc ggtccgaaga
     6121 agatcataaa gcgccccaga tccgccggat tccaacgctg cggcttttga atttgctcgt
     6181 cgtcgacgtt atcaaacggg atcgccacct gtgacacatc atacagcagg ttctgaataa
     6241 gcaagtgtaa cggcaacatc ggcaggaagg gcaagaaagc actcgccacc agcacgctga
     6301 acacattacc gaagttggag ctcgccgtca ttttgatgta tttcagcatg ttggcgaaag
     6361 tgcgacgtcc ttcaataacc ccctcttcca gcaccatcag gcttttttcc agcaggatga
     6421 tatcagccgc ttcacgggca atatctaccg cgccgtccac agaaatgccg atatccgcag
     6481 cgcgtaaggc gggcgcatca ttaataccat cgcccataaa gccaaccaca tgcccttcgc
     6541 gcttcagtaa ggtcacaatg cgttctttat gcatcggcgt cagacgagca aacagcgtgg
     6601 tgcgctgtgc gagatttgcc agttcgtcgt cagatagcgt ttcaatatca ctaccaatga
     6661 ccacctctcc cgcgtccagc cccacttcat ggcacacttt cgctgcaact aactcactgt
     6721 cgccagtaag gatttttacg gtaattccac tcgcttttaa tgccttcagt gccggagcag
     6781 ttgtctcttt tggcggatca aggaaagcaa tatatccttc gaggatcagg tcggattcat
     6841 ccgcccgctg gtaatctcct tcacgcgctg gcaggtattt cgtcgccacc gcaaccacgc
     6901 gcagcccctg acgattcagc gtatcagtaa cccgcttaat cttacgcagc atgatgtcat
     6961 cgagcggcac aatctcgcca ttgtgacgca cctgcgaaca cacattgagg atttcctgca
     7021 atgcaccttt gcaaaccagc tgatggtgct cggtattttc tgccactacc accgacatcc
     7081 ggcgacgctc gaaatcaaac ggaatctcat cgattttttg ccaacgactg gccagcgagc
     7141 gcgctgactc ttcatccgta ccttcgagca ccgctgtatc gagcaggttt ttaagtccgg
     7201 tctgataatg actgttcaac cacgcgctat gcagcacgcg ttcgctggtt ttaccggaga
     7261 tatcggtatg attctccagc acaattttgt cctgtgtcag ggtgccagtt ttatcagtgc
     7321 acagaatatc cattgcgcca aagttctgaa tagcatccag atgtttgacg atcactttct
     7381 gtttcgacag ctttactgcg ccgcgcgcca gcgtcgaggt aacaatcatc ggcaacattt
     7441 ccggcgttaa gcctaccgcg accgaaagcg caaacagtgc cgcttcccac cagtcgcctt
     7501 tggtgtaacc gttgataagc aacaccaccg gagccatcac cagcataaag cgaatcagca
     7561 gcatactgac gcggctgatc ccctgctgaa aggcattcgg ctcgctttcc tgctcactaa
     7621 cacgccccgc cagttgacca aaccaggtat tggctcctgt agcaatcacc attgcttgtg
     7681 ccgtgccgct caccacggtg gtgcccataa aacacagggt gtcacactcc agcggattgc
     7741 tgtgctccgg ctggcgagtg gtagcagctt tttctacggg cagagactca ccggttaacg
     7801 acgcctgagc gacgaacaga tcccgcgcct gcaagatacg taaatctgcc gggatcatat
     7861 cccccgccgc cagttttata atatcgccgg gcaccagctg gtcgatcggg atctccagcc
     7921 agccattttc gcctttgtcg ttaattacgc gcagcaccgt cgcggtattg ctgaccatcg
     7981 ctttcagggc atctgccgct ttagtggaac gtgcttcctg aataaagttc agcaacgtag
     8041 aaatagcgac cattagcgcg ataacgcccg cggcaaataa atcttccgtg gcgtaagaaa
     8101 tagcgccgag aatggtgagt aaaatattaa aggggttgcg atagcagacc cataaatgta
     8161 cccaccacgg cgacggttgt tgtgcgggta atttattttc accatgttgt tcgcgggcag
     8221 attccacttc cgcctgattt aacccttccg gatgggtgtc gaacgttttc cacaattctt
     8281 cttcgggcat caccgccatt ttcaggcaat gcgcacttaa ggacggcggg accaccgtat
     8341 tcactgtctg ctgcgcgcca ggcaatggat cacgatgaac cagacgggaa ggtaaatggc
     8401 gaatgagccg ggtaaagatt tctttaaaca taaggagtcc ctccgcactg tctgaaacag
     8461 tacagaaatg tcttcaggcg tggcgaagcc ttacctgaag gtaataaagc tgagcaggga
     8521 cgttaaaacg tcacggcctt acgcatccgg taacgccgtg aaaagacggg cttaccggaa
     8581 aagttttaat ctccgtcgag ggagaggcgt gggatcaggt tccatataac ctccggtaag
     8641 tgaaaaaagc gccgcaaata ataaggagaa tccaataata ttcgtagcat ctatggcgat
     8701 ataatacctg ctggcaacta aaccaacgat aaaccagact ttacccattg ctgaatgcac
     8761 gggtaaagtt aggctcaaat aattaaacaa cacgttacag gacaacagga tgcaaaatcg
     8821 gctgaccatc aaagacatcg cacgcttaag cggcgtgggg aaatctacag tttcccgggt
     8881 gctgaataac gaaagcggcg tgagccagcg cacccgcgag cgtgttgaag cagtgatgaa
     8941 tcagcatgga ttttcccctt cccgctctgc gcgcgctatg cgtgggcaaa gcgataaagt
     9001 ggtcgccatc attgttaccc gcctggattc gttgtcagaa aatctcgccg ttcaaaccat
     9061 gctgccagcg ttctatgaac aaggttacga cccaatcatg atggaaagtc agttttcccc
     9121 gcaattagtt gccgaacatt tgggggtgct gaaacggcgt aatatcgacg gcgtagtgct
     9181 gttcggtttt actggcataa cagaagaaat gttagcccac tggcagtcat cgctggttct
     9241 gctggcgcgt gacgcaaaag gttttgcttc ggtctgttat gacgacgaag gggcaatcaa
     9301 gatcctgatg caacggctgt atgaccaggg gcatcgtaat atcagttatc tcggcgtgcc
     9361 gcatagtgac gtgacgaccg ggaagcgacg tcacgaagcc tacctggcgt tctgcaaagc
     9421 gcataaactg catcccgttg ccgctctgcc aggccttgct atgaagcaag gctatgagaa
     9481 cgtagcaaaa gtgattacgc ctgaaactac cgccttactg tgcgcaaccg acacgctggc
     9541 acttggcgca agtaaatacc tgcaagagca acgcatcgac accttgcaac tggcgagcgt
     9601 cggtaatacg ccattaatga aattccttca tccggagatc gtcaccgtag atcctggtta
     9661 cgccgaagct ggacgccagg cggcctgcca attgatcgca caggtaaccg ggcgcagcga
     9721 accgcaacaa atcatcatcc ccaccaccct gtcctgatcg tttcctgaac gataaattgt
     9781 gatcttcgct gcgtttcggg aacgttcccg tttttaaatt tttccgcgca atatattctg
     9841 cagccaacca aaaatgtcat ctgccatggg gctttatgat gagcaaaata aaccaaacgg
     9901 atatcgatcg gttgattgaa ctggtcggcg ggcgcggcaa tattgcgacg gtgagccact
     9961 gtattactcg cctgcgcttt gtcctcaacc aaccggccaa tgccagaccg aaagaaattg
    10021 agcaactccc catggtgaaa ggctgtttca ccaatgccgg gcaatttcag gtggtgattg
    10081 gcaccaatgt gggtgattac tatcaagcac taatagcgtc aaccggacag gcgcaggttg
    10141 ataaagagca ggtaaaaaaa gccgcccggc agaatatgaa atggcatgag cagttgatct
    10201 ctcatttcgc ggagatcttc ttcccgttgc tgcccgcgtt gatcagcggc ggtttgatcc
    10261 tcggttttcg caatgtgatc ggcgatttgc ccatgagcaa cggtcagacg ctggcgcaaa
    10321 tgtacccttc cctgcaaacg atctacgatt ttctgtggtt gatcggtgaa gcgatcttct
    10381 tctacctgcc ggtcggtatt tgctggtcag cggtgaaaaa aatgggcggc acgccgatcc
    10441 ttggtatcgt gcttggcgtg acactggttt ctccacagct gatgaacgct tatctgctcg
    10501 ggcagcagct gccggaagtg tgggactttg gcatgttcag catcgccaaa gtaggctatc
    10561 aggcgcaggt gatcccggca ctgttagccg gactggcgct gggcgttatt gaaactcgcc
    10621 ttaaacgcat cgtgccggat tacctctatc tggtggtggt acccgtctgt tcgctgatcc
    10681 tcgcggtgtt cctcgcccat gcgctgattg gtccgtttgg tcgcatgatt ggcgatggcg
    10741 ttgcctttgc ggtacgtcac ctgatgaccg gcagctttgc tccgattggt gcggcattgt
    10801 ttggcttcct gtacgcgccg ctggtgatca ccggcgtaca ccagaccacc cttgctattg
    10861 atttgcagat gattcaaagc atgggtggca cgccagtgtg gccgctgatt gcgctgtcga
    10921 atatcgctca gggctccgcc gtaataggca ttatcatttc cagccgcaag cacaatgagc
    10981 gcgagatctc cgtgcctgcc gctatctccg cctggcttgg ggtcactgag cctgcaatgt
    11041 acggcatcaa cctgaaatat cgcttcccga tgctgtgcgc gatgattggt tctggtctgg
    11101 caggattgct atgcggcctg aacggcgtta tggcgaatgg tatcggcgta ggcggcctgc
    11161 cgggaattct ctcgattcaa ccgagctact ggcaggtgtt tgcgctggca atggctatcg
    11221 ccatcatcat cccgattgta ctcacctcgt ttatctatca gcggaaatac cgcctgggca
    11281 cgctggacat tgtttaattt tcttcggggc gcaattgcgc cccctcgcat ttgcaggaat
    11341 aacgtaatga ctaatcttcc ccactggtgg caaaacggcg ttatctacca gatttatcca
    11401 aagagttttc aggacaccac gggtagcggc accggcgatt tacgtggcgt tatccaacgc
    11461 ctggactatc tgcataaact gggcgttgat gccatctggc taaccccctt ttatgtctct
    11521 ccccaggtcg ataacggtta cgatgtagcg aactatacgg cgattgatcc cacctacggc
    11581 acgctggacg attttgacga actggtgacg caggcaaaat cgcgcgggat tcgtatcatt
    11641 ctcgatatgg tgtttaacca tacctctacc caacatgcct ggtttcgcga ggcgctgaac
    11701 aaagaaagcc cttaccgcca gttttatatc tggcacgatg gcgaaccaga aacgccaccg
    11761 aacaactggc gttcaaaatt tggcggtagt gcgtggcgct ggcatgcgga aagcgaacag
    11821 tactatttgc atctctttgc accagaacag gcggatctca actgggagaa tccagcggta
    11881 cgcgcagagc tgaaaaaagt ctgtgaattc tgggccgatc gtggggtcga cgggttgcgc
    11941 ctggatgtgg tgaatctgat ctccaaagac ccgcgtttcc ctgaagacct ggatggcgac
    12001 gggcgtcgct tctacaccga cgggccacga gcacacgagt ttttgcacga gatgaaccgc
    12061 gatgtgttta cgccacgcgg gttaatgacc gtaggtgaaa tgtcctccac cagccttgag
    12121 cattgccagc gatacgcggc actgacaggc agtgaattgt cgatgacctt taattttcat
    12181 cacctgaagg tcgattatcc cggtggtgaa aaatggacgc tggctaaacc tgactttgtg
    12241 gcgttgaaaa cattgttccg ccactggcaa caaggaatgc acaacgtggc atggaatgcc
    12301 ttgttctggt gtaaccacga tcagccgcgc attgtttctc gctttggtga tgaaggtgaa
    12361 taccgcgtgc ctgcggcaaa aatgctggcg atggtgctgc atggcatgca gggaacgccg
    12421 tatatctacc agggcgaaga gattggcatg accaacccgc atttcacgcg cattactgac
    12481 tatcgcgacg tggagagcct caatatgttt gccgagctgc gcaacaatgg gcgtgatgcc
    12541 gacgagttat tggcaatcct cgccagtaaa tcccgtgaca acagtcgcac gcccatgcaa
    12601 tggagcaacg gcgataatgc cgggtttacg gctggcgaac cgtggattgg cctgggcgat
    12661 aactatcaac aaatcaacgt agaagccgcg ctgaccgatg agtcctcggt gttttacacc
    12721 taccaaaagt taatcgcact gcgtaagcaa gaagccgttc tgacatgggg caattaccag
    12781 gatctgctgc caaacagccc tgtattgtgg tgctatcgcc gtgaatggaa ggggcaaacc
    12841 ttgctggtca ttgccaacct tagccgtggg atccaaccct ggcagccagg gcaaatgcgc
    12901 ggcaactggc agcttgtgat gcataactac gaagaagcct caccacaacc ctgtgccatg
    12961 aatttacggc cttttgaggc tgtctggtgg ttacagaagt aaatcttccc taagccccgg
    13021 taatgccggt cattccgggg ttttgtctgt cagcatgttg tttttgttga tttttcaacc
    13081 agcaaattca ttaaaaaatt tacataacgc tgtagcgccc gtcatccgta cgctctgctt
    13141 tttactttga gctacatcaa aaaaagttca aacatccttg atgcaaagca ctatatatag
    13201 actttaaaat gcgtcccaac ccaatatgtt gtattaatcg actataattg ctactacagc
    13261 tccccacgaa aaaggtgcgg cgttgtggat aagcggatgg cgattgcgga aagcaccgga
    13321 aaacgaaacg aaaaaaccgg aaaacgcctt tcccaatttc tgtggataac ctgttcttaa
    13381 aaatatggag cgatcatgac accgcatgtg atgaaacgag acggctgcaa agtgccgttt
    13441 aaatcagagc gcatcaaaga agcgattctg cgtgcagcta aagcagcgga agtcgatgat
    13501 gccgattatt gcgccactgt tgctgcggtt gtcagcgagc agatgcaggg ccgcaaccag
    13561 gtggatatca acgagatcca gaccgcagtt gaaaaccagc tgatgtcggg tccatacaaa
    13621 caactggctc gtgcttacat cgagtaccgt cacgatcgcg acattgaacg tgaaaaacgc
    13681 ggtcgcctga accaggagat ccgtggtctg gtcgagcaga ccaacgcctc gttactcaac
    13741 gaaaacgcca acaaagacag caaggtgatt ccaacccagc gcgacctgct ggccgggatc
    13801 gtggctaaac actatgcacg ccagcacctg ctgccgcgtg acgtggtgca ggcacatgag
    13861 cgtggcgata ttcactatca cgatctcgat tactcgccgt tcttcccgat gttcaactgc
    13921 atgttgattg acctgaaagg catgctgacc cagggcttta aaatggggaa cgccgagatt
    13981 gaaccgccga agtcgatctc tacggcaacc gctgtaactg cgcagattat tgctcaggtc
    14041 gccagccata tttatggcgg caccaccatt aaccgtatcg atgaagtgct ggcaccgttt
    14101 gtcaccgcca gctacaacaa acatcgcaag accgcagaag agtggaacat cccggacgcc
    14161 gaaggctacg ctaactctcg taccattaaa gagtgctacg atgccttcca gtcgctggag
    14221 tacgaagtaa acaccctgca caccgccaac ggtcagacgc cgtttgtaac ctttggtttt
    14281 ggtctgggca ccagctggga atcgcgcctg attcaggaat ctatcctgcg taaccgtatc
    14341 gcaggccttg gtaaaaaccg taaaactgcg gtgttcccga aactggtgtt tgcgattcgc
    14401 gatggcctga accataaaaa aggcgatccg aactacgaca tcaaacagct ggcgctggag
    14461 tgcgcaagca agcgcatgta tccggatatc ctgaactacg atcaggtagt gaaagtcacc
    14521 ggttcgttta aaaccccgat gggctgccgc agcttcctcg gcgtgtggga aaatgaaaac
    14581 ggcgagcaga tccacgatgg tcgtaacaac ctcggcgtga tcagcctgaa cctgccgcgt
    14641 attgctctgg aagcaaaagg cgatgaagcc accttctgga agctgctgga tgaacgtctg
    14701 gtgctggcac gtaaggcact gatgacccgt atcgctcgtc tcgaaggcgt gaaagcgcgc
    14761 gtcgccccga tcctctatat ggaaggtgct tgtggcgtac gtctgaatgc tgacgatgat
    14821 gtttctgaaa tcttcaaaaa cggtcgtgcg tctatttcgc tgggttacat cggcatccac
    14881 gaaaccatta acgcgctgtt cggcggcgag catgtttacg acaacgagca gcttcgcgcg
    14941 aaaggtatcg ccattgttga acgtctgcgc caggcagtgg atcagtggaa agaagaaacg
    15001 ggttatggtt tcagtctcta cagcacgccg agtgaaaacc tgtgcgaccg cttctgccgt
    15061 ctcgatactg ctgagtttgg cgtggtgccg ggcgtgaccg ataaaggtta ctacaccaac
    15121 agtttccacc tcgatgtgga gaagaaggtg aacccgtacg acaagatcga ctttgaagcg
    15181 ccttacccgc cgctagcgaa cggtggtttc atttgctacg gcgagtatcc aaacattcag
    15241 cacaacctga aggcgctgga agatgtctgg gattacagct atcagcatgt accgtattac
    15301 ggcaccaata caccgattga tgagtgctac gagtgtggct ttaccggtga gttcgagtgc
    15361 accagcaaag gcttcacttg cccgaaatgt ggtaaccatg acgcctcccg tgtgtcggtg
    15421 actcgccgcg tgtgcggata tttaggtagc ccggatgcac gtccgtttaa cgctggtaag
    15481 caggaagaag ttaagcgccg cgttaaacat ttggggaatg ggcagatagg ttaatcttca
    15541 ctctgcgcta agcaaatgcc ggatgcggcg taaacgcctt atccggccta catttggcaa
    15601 cggctccagg cctgataaga cgcgacagcg tcgcatcagg cattgattac cggatgcagc
    15661 ctacacgcgg actgataagc caggcgaaat aatgaattat catcagtact atcctgtcga
    15721 catcgtcaac ggccccggca ctcgctgcac cctgtttgtc tccgggtgtg ttcatgaatg
    15781 ccccggttgc tataacaaaa gcacatggcg ggtaaattct ggtcagccat ttaccaaagc
    15841 aatggaagac aagatcatta aagatctgaa tgacactcgt atcaaacgcc aggggatttc
    15901 cctctccggc ggcgatccgc tgcatccgca aaacgtgccg gatattctga aactggtaaa
    15961 acgcatccgc gccgagtgtc cgggtaaaga catctgggtg tggacaggct ataaactcga
    16021 cgaactcaac gctgcgcaaa tgcaggttgt tgatttgatt aacgtactgg tcgacggcaa
    16081 atttgtgcag gatttaaaag acccttccct gatctggcgc ggcagcagca atcaggtggt
    16141 gcatcatttg cgatgataag ttccctgtta cctgagtgca ggcaacaggg aaatgaggaa
    16201 ttaacgatac ttctggtgat aggcgttgcg ggtcgttttc agttgctctg cagcagcctg
    16261 cgcttctttt actttacctt catttgccag cttcagcgcg tcgtcaatct gaccgaccag
    16321 aatgtcgaaa ccgtggcgga aatctttcat ttccgggctg tccggtgatt tatcttcgag
    16381 cttcggcggc gttgcttttt gcgcatccag cgctgcggcg cgcatcttcg ttaacgcgtc
    16441 tttgacttgc gccgcgttat ccgctttttc gatcactttt aaattgtcgt tgagggtttc
    16501 catattgtct tcaagatcag cggcaaacga cgcagaactg aataccaacg aggagactgc
    16561 aagaatagct aacaggcttt tacgcattgc tcacttcctt ttttatttac acttcatcct
    16621 tcacactgcc tcatcgacgg ctacgctcat tcgcccgggt cacgtactgc gagtacgctc
    16681 cccggccctc attcactttc cgtctcgatg cagtgcgaat gattttgtgt aattttttag
    16741 tttatagcgc ggcaggtcgc gccagttttt tactgcccgg cgattttcat ctccggcaac
    16801 agcacagaac cacactgtat attactgcgt gtttcaatat cgttaccgac ggtgacaata
    16861 ttgcgccaca tatcttttaa attacctgcg atggtgattt cactaacggg atactgaatt
    16921 tcgccattct ctacccagaa gcccgctgca ccacgggaat aatcaccggt aatggcactc
    16981 acgccctggc ccatcagctc cgtcaccacc agcccggtgc ccatctcttt gagcatttgt
    17041 tcgaagctta gcccttgtcc ggcaatccgc cagttgtgaa taccgcccgc atgtccggtg
    17101 cttttcagcc ccagtttccg cgccgagtag ctggtcagca gccactgagt caggatgcca
    17161 tctttaataa tatcgcgacg ctcggtgcgc acaccttcgc tgtcgaatgg cgtcgacgcc
    17221 agccctttca gcagatgcgg atgctcttca atggtcagcc agtccggcag aatttgttta
    17281 cccagcgaat ccagcagaaa ggtagattta cggtaaaccg agccacccgc tatcgccccc
    17341 accagatggc caaaaagccc ggttgccact tcattggcaa aaatgactgg cgctttcatg
    17401 gtggagagtt tacgcggtga caggcgcgat aaagtacggc gagcacagtc ggccccaacc
    17461 cactctggcg tttgcagatc gctcatcgca cgaccaatgg tgtaggcgta atcacgctcc
    17521 atatcgccat tttcttcggc aattacacag ctggagagtg aatgacgcgt agagcagtaa
    17581 ccctgcaaca tgccgtggct gttgccaaaa actttgacac cgtagtggct gttaaaactg
    17641 ccgccttcgg tattggtaat gcgtttgtcc gcctgcaatg ccgcctgttc tgcgcgggcc
    17701 gccagttcaa tggcttcatc cggggaaact tccgcagggt ggaacaagtc gagatctggt
    17761 gcgtcaaagg ccagcagctc tttgtctgcc acgccggcac agggatctgg cgaggtataa
    17821 cgggcaatat ccagcgccgc ctgtacggtg cgggcaatgg cctgcgggct taaatcggtg
    17881 gatgatgcgc tacctttgcg gttctggtga taaacagtga tccccagcgc gccatcgcta
    17941 ttgaattcaa cattctccac ttcaccataa cgcgtgctta cgctaatgcc ggtggtcttg
    18001 ctgacggcaa cttccgcacc gtccgatttg cctgaggcca actccagcgc agtggaaact
    18061 gcttcttcca gaatcttgcg ctgcgcttca acttgagaga ttactttcat tgcaagtgcc
    18121 ataatgtaga gagtttctct gaagtctaac agagaaccgt ttttcagtgc gcatcttaac
    18181 tggtaacatt agcctctttt tttaaggagc ctgagatgac taagcagccc gaagactggc
    18241 tcgacgacgt tcccggtgat gacatcgaag acgaagacga tgaaattatc tgggtcagta
    18301 aaagtgaaat taaacgtgat gccgaggagc taaaacgcct tggcgcggaa attgttgatc
    18361 tggggaaaaa cgcgctggat aagatcccgt tagatgcgga tctgcgcgcg gctattgagc
    18421 tcgcccagcg tattaagatg gaaggtcgcc gccgccagtt gcagctcatc ggtaagatgc
    18481 tgcgccagcg cgacgtagag cctattcgtc aggcgctgga taagctgaaa aaccgtcaca
    18541 accagcaggt ggtgctgttc cataaactgg aaaacctgcg cgatcgtcta atcgatcaag
    18601 gtgatgacgc catcgctgaa gtattgaatc tgtggcccga tgccgatcgt cagcagctgc
    18661 gtactctgat ccgtaacgcg aagaaagaga aagaagggaa taagccgccg aaatccgcac
    18721 gccagatttt ccagtatctg cgcgaactgg cggagaatga agggtaattt tcgttgttga
    18781 tgtgagtatg gtttgccgga tgcggtgcac ctgtaggccg gataagacgc atcaagcgtc
    18841 gcatcaggca ctatgctcaa atgccggatg cggcgtaaac gccttatccg gcaatgctat
    18901 atcaggctga agccgaatta ctgcgcagct tccgccttct tcgccagacc atccagcagt
    18961 ttctgatgga tcccaccaaa accgccgttg ctcatcacca gaatatggtc gccaggctga
    19021 gcggttttca ccaccatatc tgccagcgta tccacatcgc cactccagtg tgcaggctga
    19081 acgcaggctt ctgccacttc tgccacctgc cacggaatat gcgccggttg cagcaggaag
    19141 acttcatcgg cacgacctaa tgaaggtgcc agatcgtctt tgcagatccc cattttcatg
    19201 gtattcgagc gcggctccag cacagcaata atgcgcgccg taccaccaac tttgccacgc
    19261 agcgccgcca gcgttgccag aatcgccgtc gggtgatggg caaaatcgtc atataccgtg
    19321 acgccattcg cttcaccacg caactccaga cggcgacgag cattaataaa cgaacccagc
    19381 gcgttagcgg catctgccgg cgctacacca acatggcgag ccgctgcaat cgccatcagg
    19441 ccattgtgca tattatgttc gcctaccagc gaccatttca cttcgcccac tttttcgcca
    19501 tccagcaaaa cttcccattc ggaagcatcg gtggtcagct ttttcgcctg ccagtgaccc
    19561 tgctcaccca ccagctcctg ctcgctccag cagcccatcg ccatggtctg tttcaggttg
    19621 atgtcatttt ccggccagat aatacggccc tgccccggaa cgatacgcac cagatggtgg
    19681 aactgtttct ggatcgcttt caggtcgtca aagatatcgg cgtgatcgaa ctcaaggttg
    19741 ttgaggatca gcgtacgcgg gcagtaatgg acaaatttag agcgtttgtc gaagaaggcg
    19801 cagtcatact catccgcttc gataacaaag aagtcgcttt cgcccagacg cgccgaaacc
    19861 tcaaagttcc ccggcacacc gccgatcaca aatcccggtt tgtaaccgca ctgttccaga
    19921 atccaggtcg ccattcccgc ggtggtagtt ttgccgtgtg taccggcaac ggccagcacc
    19981 cagcggtcgc gcagcacaaa atcgtgcagc cactgtggac ctgacatata agggatgttt
    20041 ttttccagta ccgcttccac acacggattt ccacgggtca tggcgttgcc aataatcacc
    20101 agatccggct gcggatcgag ctggctggca tcgtaaccct gaatcagctc aatgccttgc
    20161 ttctcaagta aggtgctcat cggcggatac acattggcgt ccgaacccgt tacttcatgg
    20221 cctaactggc gcgccagcat cgccagaccg cccataaatg tgccacaaat tcctaaaata
    20281 tgaatgcgca tacgtcacta tcctttttta atctgccgct cattttacgc atatgtcggg
    20341 caagtgagaa acgcatttca ggataatccg caatttgctg gcgcgattca cctgagcgca
    20401 acattgagat tatttgttaa gattgttgcg gtcgctttac tccataaaca ttgcagggaa
    20461 agttttatga aaacgttagg tgaatttatt gtcgaaaagc agcacgagtt ttctcatgct
    20521 accggtgagc tcactgcttt gctgtcggca ataaaactgg gcgccaagat tatccatcgc
    20581 gatatcaaca aagcaggact ggttgatatc ctgggtgcca gcggtgctga gaacgtgcag
    20641 ggcgaggttc agcagaaact cgacttgttc gctaatgaaa aactgaaagc cgcactgaaa
    20701 gcacgcgata tcgttgcggg cattgcctct gaagaagaag atgagattgt cgtctttgaa
    20761 ggctgtgaac acgcaaaata cgtggtgctg atggaccccc tggatggctc gtccaacatc
    20821 gatgttaacg tctctgtcgg taccattttc tccatctacc gccgcgttac gcctgttggc
    20881 acgccggtaa cggaagaaga tttcctccag cctggtaaca aacaggttgc ggcaggttac
    20941 gtggtatacg gctcctctac catgctggtt tacaccaccg gatgcggtgt tcacgccttt
    21001 acttacgatc cttcgctggg cgttttctgc ctgtgccagg aacggatgcg cttcccggag
    21061 aaaggcaaaa cctactccat caacgaagga aactacatta agtttccgaa cggggtgaag
    21121 aagtacatta aattctgcca ggaagaagat aaatccacca accgcccgta tacctcacgt
    21181 tatatcggtt cactggtcgc ggatttccac cgtaacctgc tgaaaggcgg tatttatctc
    21241 tacccaagca ccgccagcca cccggacggc aaactgcgtt tgctgtatga gtgcaacccg
    21301 atggcattcc tggcggaaca agcgggcggt aaagcgagcg atggcaaaga gcgtattctg
    21361 gatatcatcc cggaaactct gcaccagcgc cgttcgttct ttgtcggcaa cgaccatatg
    21421 gttgaagatg tcgaacgctt tatccgtgaa ttcccggacg cgtaatctcc tgcgcctctc
    21481 cacacggaga ggcgttttta tcgctgcgca atattgactc ttgtcactgg cgaactctga
    21541 cgattctccc acagcaccgt taatccacgc tgtaaggcaa taaaaatgaa cagcaaaatg
    21601 ccgatggcga ttttcgtcca ccaggaactc agcgtaccgt caaagttgat ataagtctga
    21661 attagcccct gaatcgccac gccaaaaagc gtccctaata ccgttccaac gccaccgctc
    21721 aaaagcgtac cgccaattac cactgaggcg atagcatcca gttccacacc tacgcccgcc
    21781 agcgcatatc cggcctgggt ataaatcgag aagacaatcc ccgccagcgt tgccagtccg
    21841 gtggagagca tataaatgcg aatagtggtg ctgcgggtag aaatccccat caggtttgcc
    21901 gacgttgcgt tgccgccaat ggcgtatacc tgattgccaa aacgggtacg atgcgcaagg
    21961 aaaataccga taaccaccac cgccagcatc aacagcccca tcgcactaag gcgaccgccg
    22021 ccggggattt tccacgcaag gcttgagagc gtgtcataaa tgggatggtt tatcggaatc
    22081 gactcttccg aaacgagata gctgacgccg cgcaaaaaga acatccccgc aagcgtaatg
    22141 ataaatgccg ggatcttcag ggcgtcgatc agaagcccca taaatgcgcc gaaggcacag
    22201 cccatcacca gcactagcgg aaacgccagc agcggcgaga ggccgaaatc gccaaccact
    22261 tttgccagaa acacgccagt aaaggcgatc accgaaccga cggagagatc gataccgcca
    22321 gagaggatca caaaggtcat gccaacggcg attatcccaa gaaaggcgtt atcggtcagg
    22381 atattgcaga tcactcttgt ggaagcaaaa ccgggaaact gcgtcaggca gtagaaatag
    22441 cccagcacaa agacgccgat ggtgatcatc agtggcaaat tacgttttat catggctacg
    22501 tactccttta atcagactga taaagcgttg cgactgaaca atcagcacac aaagcaccac
    22561 caccgctttt acgacctggt tcatctccgg cggaaagcca gaaagtaaaa ttccggtgtt
    22621 catcccctga ataatcagcg cccccacgac tgagagcagc aggttaaagc gcccgcccat
    22681 cagcgatccg ccgccaatca ccaccgcaag aatggcgtcc agctccagcc ataacccggc
    22741 gttattggca tcggcaccgc gaatatccgc cgccacgata atgcccgcaa tcgccgcaca
    22801 cagcccgctc aacacgtagg tgagcatgac gatgatccgc gtgtttaccc cggcattttt
    22861 tgccgcccga atgttgatac caacggcttc gataaacatc cccagcgccg ttttgcgggt
    22921 caacagccag aacaggagaa gcgtcagcac cgcgataatg accggcgttg gcaggaacaa
    22981 cagcgatccg ctgccaaacc atgagagatc cggcgagtta aacgtgacga tctgcccggc
    23041 ggtgatcagt tgcgccacgc cgcgcccggc gaccatcagg atcagggtgg cgacaaacgg
    23101 ctgaattttg aggatcgcta ccagtatgcc gttccacaat cccgccagga tgccggtgcc
    23161 cagggcgctt aacaaaacaa tcggcaggct gaatcccgcg accgtcatcg cagccgttgt
    23221 ggctccggcg atagccatca ccgcccctac ggagagatca atcccaccgg tggcgatcac
    23281 cagcgtcata ccaatcgcca gtaacgcaac gggtgctgca cggttaagaa tgtctatggg
    23341 gctaccgaac aaacgcccat cctgaagcac tacctgccag aaatgcgggg ctaccaggct
    23401 gtcgaccagc agcaccagca atagtgctac cagctgcgac attcccgttg gccagcgaaa
    23461 gcgccttttc ggcggcgtag tgtccgggag agattgaggc atcacactgt tctccttacg
    23521 ccgcaatggc gttcatgatc gccggaacgg aaagctctgc cagcgggatc tccgccacct
    23581 gtttgcgatc gcgcatgatg atcacccggt cggcatagcc caccagttct tccagttcgg
    23641 aggagatcac cagcagcgcc agaccatcgg cgcatagcgt ttcaatcagg cggatgatct
    23701 cggcgtgggc accaacatca atgccacggg ttggctcatc gaggatcaga aattgcggac
    23761 gagtcagtag ccaacgtgaa agcaatactt tttgctgatt gccgccggag agaaattcaa
    23821 tcggttgttc agttgaaggt gtgcgaatgc caagctggcg gataaagcgt tcggcaattt
    23881 cttgctgttc tttacgggaa atgggtcgta gccagccgcg ctgggcctgg agagcgagga
    23941 tgatattttc ccgcaccgag gcggcagcga tgatgccatc ggttttcctg tcttccgggc
    24001 agaagccgat gcccagtacc gaagcctgat gtggcgatcg cagggtttgc ggtttgcctt
    24061 tgatcaacgc cgtgccgctg tcagcaggtt tgataccgaa gatcacttcg gcggtttcgg
    24121 tacgtcctga tcccagcaag cctgccaggc cgacgatctc gccggggcgc acttcgagat
    24181 caaacggtgc gatcgttcct tttttgccgt aatttttgaa cgcggcaacg ggtttgtcgc
    24241 tcaacaatgt tcgcccggca cgctgtagcg cgtgggtatc cagctcgcgc cccagcatca
    24301 tttttaccag ttcgatctgc ggtagctcgc gcgtttcccg acagcctacg aaactgccgt
    24361 tgcgtaagac ggtgatccga tcgctgacct gatagacctg atcgagaaag tgagtgacaa
    24421 agatcaggct gacgccgcga tcgcgcaact gacgcatcag gccaaacagt aattctactt
    24481 cctgagtgtc gagactggcg gtgggttcat cgaggatcag cactttggca gagagatcga
    24541 tagcccggca aatggcgacg atttgctgca tcgcgactga aaagcggtta agcggttcac
    24601 gcacgtcgag ggagaaaccg taagatgtca tcagttcggt ggcacgcttt tccatctctt
    24661 tgcggcgtag aaagccgaaa cgtttgggtt cgcgacctat gaatagatta tcagcgaccg
    24721 acatattggg tagcaggttg acttcctgat aaacggttcc gatgccgagt tgttgtgcat
    24781 gggcggtatt tttcggtgag atagcctggc cttccagcca gatggtgccg cgatcggcgt
    24841 ggtatacacc ggttaatgct ttgattagcg tcgattttcc cgccccgttt tcaccgagca
    24901 gcgccatgat ttcgccacgg cgcaggctga aatcaacgtt gtctaacgct ttgacgccgg
    24961 ggaaaaattt acttaatcct tcggtgcgga ggatttcctg gtgttggtcg gtcgtcatgg
    25021 tttccccctt agccacgctc taaaagtctg ggaatagatc gtgcctgatg ttaagagctg
    25081 cgccgtttgt cctcctcacc ctaaccctct ccccgaaggg gcgaggggac agattgtgca
    25141 cggtgttaag tccggtgttg caccactgcc ggatgcggcg tgaacgcctt atccggccta
    25201 cacagagcac cgaactcgta ggcatgataa gacgcggtaa gcgtcgcatc aggcttcagc
    25261 gcaccaccgc cggatgcggc gcgagcgcct tatccggcct acgaatcggc atcaggcata
    25321 gcaaccctca ataccccata tttttcttct tctctaactc ttcttttgcg gtatcaggca
    25381 ggtaaagggt ggatttggtt aacgtcagct tttcaggcat ggtgccgtct tttttgtatt
    25441 tctccagcgc gtcaaaagcg ggtcccgcca tattcggcgt cagttcaaca ctggcgttcg
    25501 cttcgccatc aatcatcgct ttgtagatgt ccggtacacc gtcaatggaa cccgtgagga
    25561 tatctttgcc cggtttcagg cccgcttctt taattgcctg aattgcgcca atcaccatgt
    25621 catcgttatg ggcgtaaacc atgcagatgt ttttgccgtt gttttccgct ttgataaagc
    25681 tttccatgac ttctttgcct ttactgcggg taaagtcacc tgactgcgag cggataattt
    25741 tgatatttgg cgcattctta atggcttcgg caaagccttt cttgcggtca atggcgacgc
    25801 tggccccaac ggtgccctgc agctccacca cgttgcatgg tttgccattc acttctttta
    25861 ccagccagtc accgatcaac ttaccttcga ggatgttgtc ggcagtaacg gtggtcatat
    25921 agagagattt gtctttcaca tcgatggaac ggtcgagcaa gaagaccggg atttcggcat
    25981 ctttcgcctc ttttaatacc ggttcccaac ctgtcgcgac caccggagcg ataaagatcg
    26041 catccacccc ttgcgcgacg aaggaacgta ccgctttaat ctggttttcc tgcttttgct
    26101 gaccatcggc aattttcagc gtaattccgc gtttttcggc ctcacttttc gccacattgg
    26161 tttctgcagc gcgccagccg gattccgatc cgacctgcga aaatcctacg gttaatggag
    26221 cggccaacgc catagacgac atggctgccg agactgcaga gactacaagt aagcgtttcc
    26281 acatagggtt gtcctcgtcg gggtaattat tattggtgaa aagatgttcg cggaaaaact
    26341 atagacaatt cgttatgtaa cggattgcgt tacatcacac ttcataaaag taaataaaac
    26401 gttaatcaca agtttgtaat cgctttcatc tcactatgaa aaatgcggct acggttatgg
    26461 attttcctgc tctgtatacc gtcttaaaac tggcgaaaaa ggaaaatgaa gacgaaaaca
    26521 agcgaagaca ttcggcgcga gttggctata atactcggca cttgtttgcc acatattttt
    26581 aaaggaaaca gacatgagct tactcaacgt ccctgcgggt aaagatctgc cggaagacat
    26641 ctacgttgtt attgagatcc cggctaacgc agatccgatc aaatacgaaa tcgacaaaga
    26701 gagcggcgca ctgttcgttg accgcttcat gtccaccgcg atgttctatc cgtgcaacta
    26761 cggctatatc aaccacactc tgtctctgga cggtgacccg gttgacgtac tggtcccgac
    26821 tccgtacccg ctgcagccgg gttctgtgat ccgttgccgt ccggttggcg ttctgaaaat
    26881 gaccgacgaa gccggtgaag atgcgaaact gattgctgtt ccgcacacca agctgagcaa
    26941 agaatacgat cacattaaag acgttaacga tctgccagag ctgctgaaag cgcagatcgc
    27001 tcacttcttc gagcactaca aagacctcga aaaaggcaag tgggtgaaag ttgaaggttg
    27061 ggaaaacgca gaagccgcta aagctgaaat cgttgcctcc ttcgagcgcg caaagaataa
    27121 ataagttctt atagcgcaat aaccctgaac accgggcttc ggttagtaag ggttttttta
    27181 tgcccgcgat aaataaacac accttattcc accaccgcct gcaagcgtaa taacgcctct
    27241 tccaccacct catccgcagc cagaccaata cgctttgcca gccgggcgcg tagatccatc
    27301 atccgcacct gattcaccag caccacgccg tgcacatcgc cttcttcgca atgtaaagga
    27361 acgctaaatc cggcataacg ggcaaaattt ccgccctgcg taatgggggc caccagcgtc
    27421 attcccagtt gattaaaggc ttgaacggag agcacaagcg caggtcgacc agcaccttgc
    27481 tgttcatggc cgcttgctgg atcaaagcca accagcacaa tgtctccccg ttcaaattca
    27541 cttttcttta ccatatttcg tcacccgcag gggtggattt accccagaca tcctgctcgc
    27601 taagttccgc ggcgttcatg tcacactgtg ccagcagttc atcaagcgag tagcgcctgg
    27661 agatgggtgt cagaatcagt tggttgttgc tcacctgcgc ttccacgctc tgccccggct
    27721 gtaagttaag ttctttcatt acgatattgg gaatgaccat acctgcactg ttcccccatc
    27781 tttttatggt aatacgcatc tgcacctcct taagttatac aaagtataac tcacggtgct
    27841 acaggaaacg ttttacccct gcacatttgt gaacgccagc agagtttttt atgcgattgc
    27901 agatatggaa agcggctcgg agagaaaagc aaaagatgga aaagttacaa aaaaaaatcg
    27961 cgagatacat cgcataaaac aacgccaccc gaaggtggcg tgcttttatt attacttatc
    28021 cctgtctaac cagtcgccgc tttcaattag ctttaatcca tcgacgggtc gttgataaac
    28081 gtacatccat gcactcccgt acggcgtctg aatcaactgg cgcgcgtatt caccgcccct
    28141 ggtgcgcaag gcatcaagtt cggccagcgt ggcgttgtca atacgataaa cttcaccgtg
    28201 tactgttccg ttccccggaa ctgcgcctgg atagtggccc aggctataca actggtagtt
    28261 atcgatactg aaatcgccca gtaactgggc attggtcatc cagtgactgt tgccttgttt
    28321 gtggcgtaaa ctgccgtaga caaatattcg cattgctaaa actcgaactg atagagcaaa
    28381 tccagtgcct ggtctacacc agacacggct tccagatata gcttaggcat caggcgataa
    28441 cgtaacgtga gtgttgctat agagtcaaat atacccacgc cgtatttcac ttgcagacct
    28501 ggcaatacat agccgctgac cactacctgg gaggagtcgc ctactccctg ggtgtcgagc
    28561 gctaaattgc ttacgccaaa cgtctcgccg attttaccca caatctggcc actttgcgca
    28621 acccccaggc caatcagcat tgaggtcatc gccgcactgt cgctctgatc gctctccagc
    28681 ccttgtccac gtagcaaata agacaatgca gcttgttgcg acatagccgg gtcagagaag
    28741 atctccgctt tcggttcgtc cgccagacca gtgacgcgaa ctccggcgat tacgtcgtct
    28801 tctgtagcat ccgggttacg aatagcttca atattaagat acggttgatc tggcggacca
    28861 gagaacagta gctcaccctt acgcacaatc agatcctgac cataggcatg gaagcgccct
    28921 tcagggatgt tgatctgccc gttcaggccc agcccttgtt tgtcctgaac gacgttaaga
    28981 tcgcccgtca gccgcgcttt caggccaaag gcgtcaatgc gcacattgtt gccaacgtgg
    29041 acaatcaggt tactgttaat cggaatcgac gccgttttcg gctcttccgg ttgcaggtta
    29101 tcgttaagca tcaccacatc gctggagacg cctactgcgc tttccggcag atcgtgcacc
    29161 acgatgcgcg cccacgggac atccacgcga ccatcgaggg taaacaggtt tggtgtagcc
    29221 tcgaatacaa catctggcga tacatccatt cgtaccatcg gcggcacggt gatccgcact
    29281 ttactgcctt tcgccgttac tcgtgcccgc cagttttcaa tttggctcca gtcggcatca
    29341 ccgttcaggt agatttcacc ctgctgggtc cgtactgtac cggcaagcgt cgagcgcata
    29401 ccgttaaagt tgaccgcaag ctggctcggc tgcatatcaa acggcataaa gttgccgtcg
    29461 atatccacac ccgtaacctg aagctgacca aacaactgcg ggctttgcac atcaccaccc
    29521 agacgcaagt tggcactcac catccccgct gctttttccc cacgggtaaa gatggggttt
    29581 atcatcgcaa ggttgaagtt acggatattg acgttgccac caagattacg gcggccttgc
    29641 ggatcggtca cctgcacctg tccatcaaac tggccgttat tggtcaggcg gatggtccag
    29701 cccaattcgg cacggttgtt acgcaattcc gccgtcagat tcagtgtctg aaacgccacc
    29761 ggcagcgccg catcgttgac ggtttgcgtt acctgcacgt tacgccccga aagggtgata
    29821 ctgccctgcg gcagcccctc tttcgtggtg tcccaggcaa catccgcttt acccgtgaag
    29881 ataccgctgg cctgagtggt ttctggcata aacggtttca gcatggcgag gtcgaagcgg
    29941 ttgagattca ccaccgcacg cccttcggcc cccgcatcga tagtttgcgg cacgcacagt
    30001 tccgcattcg ggttaagcca gcagtgtggc ccgatgctga ttttttgctc cttattgcgg
    30061 taatccagcg caatatcgcg ggtcagcgac cacgggccga ccggcgtctg gaagcgggta
    30121 ttgctaagag ttcccttcca gcgttcttct ttgcgatcaa aacttcctgc cagattaagc
    30181 tgcccggaga caggctcgcc ctgaatccgc aactgtagct cgtgctgctt ttcgctgcct
    30241 ttggcattca gggtgacgag gttgatattt acatccggct gcgaaatttg ctcaacgcgt
    30301 acgtcgagtt tcccggcgat ctgatcggtg gatttaatgt cgccttccac gcgaacctgc
    30361 gccacggaaa gttcctgcca gcgcaggccg cgcgcggtga tatctgccag tagttgtggc
    30421 gcttccaccg tgccgcgtac tttcaccagc cctttcgctg taccgccaag ccccggcagc
    30481 gcgttatcca gccccggcgc gttgatggtg gcatcaagat tgagatcttt tacccccagc
    30541 tcgcctttca cttcggcact gtttggcccg agttccagat gaagcccagg gatcatccac
    30601 tgcatataac tgttgccttt cagcgtgccg tcaacgttca ctttgttctg tttgacgtta
    30661 ccggtcagct tcagttctgg cacctccatc tgccaggtgc cgccgtacag gctaccgcgg
    30721 gttttaatca agccattgag tttcgacggc cactccggga actctttggc ggtgttaatg
    30781 ccattaagcg ttagctcacc gcgccaacta atggcctgct gccagtcgag caacgccttg
    30841 agttcagttt tcccttccag cgccgcgacg gtgagtttgt cgagattgac ctgctgttca
    30901 ttacctttgg catcaagggt aatggtggct ggcgggatct cctgtccctt cactgccgta
    30961 cgcatagaga gcgtgtaatc ggtcattttg ccggtaagtt tcagtttcag atcatccgcc
    31021 tgatactgct tctcaccagt gaacggccag taaagctgtt tgctgttcac ttccacgttg
    31081 agcggcaatc cggcttccgc cagtcgcgtc tgggcgcgta aatccatatc caccggaccg
    31141 gaaagattaa cgccaatctc cagctgttcg cgcagcgcgc cgcccacttt tagcttcact
    31201 ttttcacctt tcaacggctc cacgttcagt gtgctgttga gggtgatatc caccggccag
    31261 ttgtctgaca gctgcgccgt gccgctggcg ttgacgatcc cctggttgga atcgatatcc
    31321 agggcgtcca gtttagtatt gccgtcaatg ctgctcactt tcagcagcat ggtgcgcacg
    31381 gtgatgtccg tgtcgcccgt cacgcgtaac tgctcgcctt taaactcttc aatgttcagg
    31441 ttaagcggca aatgcaagtc ggtcatttcc ggcaataccg ggcgagaaaa gagatctttc
    31501 agcgtttcgc cgagcggctt ttcctccggc tgcggatttt caattttcgg ttcgaccact
    31561 tcttcctgcg ccacttccgc cactttcggc agagcaatca gcaggccttt cagcgacgtc
    31621 ggtttcaggg tcagggtttt ctcctgccag ttcaggccgg aggtgaagtc catcaccgac
    31681 accgtggtgt catcaatctt gatgttgacg ttgtccagcg ccacccgtgt cagggtgatg
    31741 ggatacggcg tggagagatc cagcggaccg ctatcttcct cttcttcaac ctgttcagaa
    31801 ggaggcattt ttttactgtc gatgttgacc tgaatgtctt tcagcgccag gtcattaata
    31861 caaacgctac tgttccacag gcactcaaga ccgaccgcca gatgcagatt acccgctttt
    31921 accgccacgc ctggctgctc ataacgaacg tcagacaagg tgagatcgcg ccagccgccg
    31981 gtgaccttgc caatatccag tcctggcacc cagcgatccg ccgctttaaa tacaagatgc
    32041 aggccgctgg tggtgcccac cagaaacgcc accgatccca gcaacagtaa gataacgata
    32101 accacgccga ggctgatttt tttccataaa ctcataattc tggccccaga ccgatgtaaa
    32161 actgtaaccc gtgttcgtct ttatccgcga ccggtacggc aaaatcgagt ttgattggcc
    32221 cgaccggcga ttcccagcgt acgccgaccc cggtaccggt tttaaagtcg ctgcggcgaa
    32281 tatcgcttac cgcttcgcca ctatcgacaa acaccgcgcc ccaccatttt ccggtcacgt
    32341 tgtactggta ctccagcgat ccggttatca acttcgaggc ccctttcagg tcaccgttgg
    32401 cgtatttcgg ggcgatagat ttgtatttgt agccgcgaat actgcgatcg cccccggcga
    32461 agaaacgcag atccggcggt actttgtcga aatcaccggt ttcaatccag cccagcgtgc
    32521 cgcgtgtaac aaaacgatgg cgatcgtaca gtgtgcggat ccagacgttt tgtgcctgga
    32581 aaacggagaa atcgacatct gagccccagg ccgtgttgga gtagtcgata gagtagcgtt
    32641 gcgagtcgcc ccaggttggc atcaagccac cacgagaacg cgtgcggcta atcatcaccc
    32701 ctggataaaa cagcatcgtg gtgttggtaa tttcaccctg agtaaagtgg tcgagactcc
    32761 agcgcaggtt aatggcacgc tgccagccgc tggagagatc ccagtagcga gaagccacca
    32821 gcgtagtgga gtcagactcg gtatcgttca ggtcagtgcg cttaaaaccg ccctgcacca
    32881 aataatattg ttccagtgga ttcttcagca gcggcatttt atagctgaag tcgagggtct
    32941 gttccggcgc ggaaatactg gtactggtgg tcagactgtg accataagag ttcatccacg
    33001 gctttttcca cgtcgctttc acgcgcggtc ccacgtccgt agagtaaccg accccggttt
    33061 cgatggtatt ttcagttcgc ggcgaaacca cgcccgtcaa tggtaatact ttcgtttcgc
    33121 gcgctttatc aaattgtgga gccactacga ccgagttaaa ccagccggta gcagaaagtc
    33181 gacggttcag ttccgccaga tctttcgatt cgtactcatc gccctcttta aacggcacca
    33241 gattttgcag gtattcatcg cggatttgtg atccttcaaa ggtcacatgc ccaaagcggt
    33301 aacgttcgcc actgttataa tcaatatccc agaaggcttt atgcaggccg agcgcaatgc
    33361 ccagctgcgc tttggtaaat tcgctatcga aataaccttt acgcaacgca atgctggtta
    33421 aggacttttt gaaattttca taatcgccct ggttcagtac cgtgccaata gccgggcgag
    33481 tatcgagcaa tttcaaatag tctttatcgg tccgcgcgcc gccgcgcaat accacatcgg
    33541 tgccgccaat taacaccggc acgcctggcg tgactttggc gatcaatacc tgccgccctt
    33601 tctttggcgg tggacggaga tcaaattcaa tggtcggctg gtaataaccc agcgctttca
    33661 gaccttcgcg gatggcatca tcgacgcgtg cgcgaaagcg acggtctggc gtcacttcat
    33721 cactttcaat cgtagaaagc tgcgcacgaa cgttcttttc cagctgtccc gataacccct
    33781 cgacctgtag acggacgttc gcggcgacgg cagatccgct taagcagagt aagcttacac
    33841 agcataactg tcggatatag cgcacatttt ctcctgaata tccttttttc ctgcccctgg
    33901 aacgccgtta aacggcttaa caaaaatcca gtaatatgga ttaaaaagca gactaaaccc
    33961 caaatatttc ttatgtttta ctttagacct attcacggtg gttattgtgt gcaaatacgc
    34021 ctcttgttac aaccttaacc ccaatgaccg attttcggga gagcgacacc atgagtttat
    34081 ttgataaaaa gcatctggtt tcccccgccg atgccctgcc tggacgtaac accccgatgc
    34141 ccgtagccac gctgcatgcg gtcaacggtc actcaatgac caatgtacct gacggaatgg
    34201 agattgccat ttttgcgatg ggttgtttct ggggtgtgga gcgtctgttc tggcagttac
    34261 ccggcgttta cagcaccgcc gcaggctata ccggcggcta tacgccaaat ccgacttatc
    34321 gggaagtgtg ctccggtgat acgggtcatg ccgaagcggt acgcattgtt tacgatcctt
    34381 ccgtcatcag ctatgagcag ttgctacagg tattttggga gaatcacgat cccgcccagg
    34441 gaatgcgtca gggcaatgac cacggcacgc agtatcgttc agcgatttat ccgctgaccc
    34501 cagaacagga tgccgcagct cgcgccagtc tggaacgttt tcaggcggcg atgcttgccg
    34561 ccgatgatga tcgtcacatc accacggaaa tcgctaacgc cacaccgttt tattatgccg
    34621 aagatgacca ccagcaatat ctgcataaaa acccgtatgg ttactgtgga attggcggaa
    34681 ttggcgtctg tctgccaccg gaagcatagc gttacgggta caaatgtaga ttgttgataa
    34741 agtgcgcttt atttatgcca gatgcggcgt aaacgcctta ccaggcctac aaaatcgtcc
    34801 aaattcaata tattgcaagg actgcgtagg cctgatcggc atagcgcatc aggcagtatt
    34861 gcatttatca gcagtctgaa ttttaaccct ctggcgactt tacagtacct tacgctatac
    34921 tagccactga aaatgccggt tcactttctt cgaatcggct ttcaatgtgt atttcacaca
    34981 aattaatcaa cttccccttc cgaggatctg gcctgaaagg tcggataaga tatgttaaac
    35041 agtattttag tcatactctg cttgatcgct gtaagtgcgt tcttctcgat gtccgagatc
    35101 tcacttgccg cctcacgcaa aatcaaactt aaactgctgg ctgatgaagg caatataaat
    35161 gcccaacgcg ttctgaatat gcaggaaaat cccggcatgt tctttaccgt ggtccaaatc
    35221 ggtctgaacg cagtggcgat tctcggcggt atcgtcggtg atgcggcatt ttctccagct
    35281 tttcacagcc tgttctcccg ctatatgtcg gcagagctct ctgagcaact gagctttatt
    35341 ctctctttct cgttagtgac tggcatgttt atcctgtttg cggatttaac cccgaaacgc
    35401 atcggtatga ttgcgccaga agctgtcgct ttgcgtatca tcaacccgat gcgcttctgc
    35461 ctgtacgttt gcaccccgct ggtgtggttc ttcaacggcc tggcgaacat gatcttccgt
    35521 attttcaaac tgccaatggt acgtaaagat gacatcactt ctgatgacat ctacgcggta
    35581 gtggaagccg gtgcgctggc gggcgtgtta cgtaaacagg aacacgagct gattgaaaac
    35641 gtctttgagc tggaatcccg taccgttccg tcttcaatga caccgcgtga aaacgtgatt
    35701 tggtttgatc tccacgaaga tgagcaaagt ctgaagaata aggtggcgga acatccgcac
    35761 tctaagttcc tcgtctgtaa tgaagatatt gaccacatca tcggttatgt cgattctaaa
    35821 gacctgctga accgcgtgct ggctaaccaa agcctggcac tgaacagcgg cgtacaaatt
    35881 cgcaacacgc tgattgtgcc ggatacgtta accctttcag aggcgttgga aagttttaaa
    35941 accgcaggtg aagacttcgc ggtgatcatg aacgagtacg cgctggtggt ggggatcatc
    36001 accctcaatg acgtgatgac cacgctgatg ggcgatctag tcggtcaggg gctggaagag
    36061 cagattgtcg cccgtgatga gaactcatgg ctgattgacg gcggcacccc aattgacgac
    36121 gtcatgcgcg tgctggatat tgacgagttc ccgcagtcgg gcaactacga aaccatcggc
    36181 ggctttatga tgtttatgct gcgtaagatc ccgaaacgca ccgattcggt gaaattcgcc
    36241 ggctacaaat ttgaagtggt ggatatcgat aactaccgca tcgaccagct gctggtgacc
    36301 cggatcgaca gcaaggccac cgccctttcg ccaaaactgc ctgacgctaa agataaagaa
    36361 gaaagcgtcg cgtaaccccc agaaacatca acggctcctg aatcaggagc cgtcttatta
    36421 ctgcatagca ctttggttaa gccatctctg tttgcagacg cataacctga cggttaactt
    36481 cggacatcac tgacaaatgc agcttatcct tcacttttgg gataagaatc ttacccttat
    36541 caaattcaaa agcgccaacg tccttgatgt ataaccgtcc acggaacagg atctttacgt
    36601 acttcgccac ctgaagtggg ttgtaccgtt ggaaaatttt cattgttatc tcctgctgag
    36661 tattacgcct gtgcggtgcc acaatcggcc caactattat gaggcgcaaa ttttaatgct
    36721 tagtgactat agactatccg ggcaatgttt ccaccgcgta taactttttt tacctaaagg
    36781 ttacaattat tcagaattat ctttttaccg aagcgcggtc ttcagtataa gcattcattt
    36841 ttcatatgat ttgtgcgctt gaccgcaaac tggcatcaca cttgcgggaa attcgataaa
    36901 tagcacatat gattaaaact cagacccaag tggtcggatc acctgcatat cataagaagg
    36961 aaacaccatg accctacgca agattctggc actcacctgc ctgctgttgc cgatgatggc
    37021 ttccgcacat cagttcgaaa ccggtcagcg agtgccgccg attggcatta ccgatcgggg
    37081 cgagttggtg cttgataaag atcagtttag ctacaaaacc tggaacagcg cgcagttagt
    37141 gggaaaagtg agagtactgc aacatattgc tggtcgcacc tcagcaaaag agaaaaacgc
    37201 gacgctgatt gaagcgatta aatcagcgaa gttaccgcac gatcgttacc agaccaccac
    37261 cattgttaac accgacgacg cgattccggg ttccggcatg tttgtgcgca gcagtctgga
    37321 gagtaataaa aagctttatc cctggtcgca gtttattgtc gatagcaatg gcgtcgcgcg
    37381 cggtgcctgg cagctggatg aagaaagttc cgctgtagtg gtgctggata aggatggtcg
    37441 cgtgcaatgg gccaaagacg gggcgctcac acaggaagag gtgcagcaag tgatggacct
    37501 gctgcataaa ttaattaata aatagacact ctgaaccccg gattcaggaa cgactcgcgc
    37561 ggagtgtaat ccagcggttt accctgccag tcgtgaacgt gcgctccggc agccgcagcc
    37621 acagcatgac cagcggcggt atcccaaata ttcgttggcc cgaaacgcgg gtacagctgc
    37681 gcctgtcctt ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt
    37741 tcgccaagct gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc
    37801 accagcggcg ggcgcgcatc gcggacctga atctgcttgc gcacaccgca ctcttctttc
    37861 caggccttgc cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct
    37921 aaaatcggtt tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta
    37981 ataaactctt tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg
    38041 acttcccaac cgggaggatc ttcttcagaa aggaccggaa tatccggtgt cagcgtacgt
    38101 aaaccgtcca tgataacggt gtgtgcggca atatccgctg ccgttaccgg agaattgtcc
    38161 gctttgctga cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct
    38221 gcattccgtg caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg
    38281 tgttaactta ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt
    38341 catcattttc aaagcgtaaa attgtggcat tcttcactgt tctataagta agacgtttat
    38401 tctccctttt ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg
    38461 caacgctcct ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta
    38521 tcatggaaac cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg
    38581 ccacggaaaa attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag
    38641 tgaaaaacag cgtactggtc gataacggcg atttgattca ggggagtccg ctggccgatt
    38701 acatgtcggc gaaaggatta aaagcaggtg atgttcatcc ggtttataag gcgctgaata
    38761 cgctggatta tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc
    38821 tgaaaaatgc gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca
    38881 gaaccaaaca gccaatgttt acaccgtatt taattaaaga caccgaagtg gtcgataaag
    38941 acggaaaaaa acagacgctg aagattggct atattggcgt cgtaccgccg caaatcatgg
    39001 gctgggataa agctaattta tccgggaaag tgacggtgaa tgatattacc gaaaccgtgc
    39061 gcaaatacgt gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcacatt
    39121 ccgggctgtc tgccgatccg tataaagtga tggcggaaaa ctcagtttat tacctcagtg
    39181 aaattccggg cgttaacgcc attatgtttg gtcatgctca cgccgttttc ccgggtaaag
    39241 attttgctga tatcgaaggg gctgatatca ccaaaggcac gctgaatggt gttccggcgg
    39301 taatgccggg catgtggggc gatcatcttg gggtggtcga cttacaactc agtaatgaca
    39361 gcggtaaatg gcaggtgacg caggcgaaag cggaagctcg gccgatttac gacatcgcta
    39421 ataaaaaatc cctcgcggcg gaagacagca agctggtaga aacactcaaa gccgatcacg
    39481 atgccacacg ccagttcgtc agcaagccaa tcggtaaatc tgccgacaat atgtatagct
    39541 atctggcgct ggtgcaggac gatccgaccg tgcaagtagt gaacaacgcg caaaaagcgt
    39601 atgtcgaaca ttacattcag ggcgatccgg atctggcaaa actgccggtg ctttcagctg
    39661 ccgcaccgtt taaagttggt ggtcgcaaaa atgacccggc aagctatgtg gaggtggaaa
    39721 aaggccagtt gaccttccgt aatgccgccg atctttatct ctaccccaat acgctgattg
    39781 tggtgaaagc cagcggtaaa gaggtgaaag agtggctgga gtgctccgcc ggacagttta
    39841 accagattga tcctaacagc acgaaaccgc aatcactcat caactgggat ggtttccgca
    39901 cctataactt tgatgtgatt gatggtgtga attatcagat tgatgttacc cagcctgccc
    39961 gttatgacgg cgagtgccag atgattaatg ccaatgcgga aaggattaag aacctgacct
    40021 ttaatggcaa gccgattgat ccgaacgcca tgttcctcgt tgccaccaat aactatcgcg
    40081 cttacggcgg caaatttgcc ggtacgggcg acagccatat cgcttttgct tcaccggatg
    40141 agaaccgctc ggtgctggca gcgtggattg ctgatgagtc gaaacgtgcg ggggaaattc
    40201 acccggcggc agataacaac tggcgtttag caccgatagc tggcgataag aaactggata
    40261 tccgtttcga aacttctccg tcagataaag ccgcagcgtt tattaaagag aaagggcaat
    40321 atccgatgaa taaagtcgcg accgatgata tcgggtttgc gatttatcag gtggatttga
    40381 gtaagtaaaa cacttctttt tcggcctata aatcatcaac cgcatccggc atttattggc
    40441 ggatgcgatg ctggcgcatc ttatccgccc tacaagccat gcaccgtaga ccagataagc
    40501 tcagcgcatc cggcagttat gccgcacgtt catcccgcac cgccaacacc tcgggcaaat
    40561 tcaactcaat ccagtccgcc agtgcagcaa ccttttcgct cacctgctcg cccagcggcg
    40621 tgaggctgta ttccacatgc ggcggcacca ccggatacgc gatacggtta agaaaaccat
    40681 cctgttctaa cgcctgtaac gactgcgcaa gcatcttttc actcacccca ccgattttgc
    40741 gccgcaggtc gctaaagcga tgagtacctt cgcgtagcgc caccagaatc aatacccccc
    40801 aacggctggt gacgtgtttc aacacctcgc gcgacgggca ctgttccgca aagagattac
    40861 cctctttcag ttgttgcgac aggctaacct gactcatttc atacttacct ttttgtacgt
    40921 acttactaaa agtaagttta ggtgttagcg tatttaaaca caagacaaaa cgatggagac
    40981 ttcccatgat cgctattact ggtgccactg gccaacttgg tcactatgtt attgaatcct
    41041 tgatgaaaac ggttcctgcc agccaaatag tggctatcgt tcgtaatccg gcaaaagccc
    41101 aggccctggc agcacaaggc attaccgtgc gtcaggctga ctacggcgat gaagccgcac
    41161 tgacatctgc acttcaggga gtggaaaaac tactgctgat ctcttccagc gaagtgggtc
    41221 aacgtgcccc gcagcatcgt aatgttatta atgccgcaaa ggcggctggc gtgaaattta
    41281 tcgcttatac cagcctgcta catgcagata cctccccgct cggcctcgcc gatgagcaca
    41341 tcgagacgga gaaaatgctg gctgattctg gcatcgttta caccctgctg cgcaacggct
    41401 ggtacaccga aaactacctc gccagcgccc cggcagcact ggaacacggc gtatttatcg
    41461 gtgcggcggg cgatggcaaa atcgcctcag caacgcgggc agattatgcg gcagctgcgg
    41521 cacgcgtgat tagcgaagcc ggtcacgaag gcaaggttta cgaactggcg ggcgatagtg
    41581 cctggacgtt gacacagtta gcggcagagc tgaccaaaca gagcggaaaa tccgttacct
    41641 atcaaaatct gagcgaagcc gatttcgccg cggcactgaa aagcgtcgga ctgcccgacg
    41701 gactggcgga tatgctggcg gattctgacg ttggcgcatc gaaaggtggt ctgtttgata
    41761 acagcaaaac gcttagcaaa ttgattggcc gcccaacgac aacgttagcc gaaagcgtaa
    41821 gccatctttt taatgttaat aactagttaa ttaaagtggc atcctcccgc atcctctctg
    41881 ataatgacgg gatgccggga gcaatcatgt ctgcttcctg aactttcttc tgacagacca
    41941 atggatgcca gtaatgatta gcggcgtgct gtacgccctg ttagcagggt tgatgtgggg
    42001 gcttattttt gtcgggccgt tgatcgtacc ggaatacccg gcgatgttgc agtcgatggg
    42061 gcgttatctg gcgttagggt taattgcgct gcccattgcc tggctgggac gcgtgcgtct
    42121 gcgtcagttg gcgcgtcggg actggcttac cgccttgatg ctcactatga tgggcaacct
    42181 catttattac ttctgccttg ccagtgccat tcaacgtact ggcgcgcctg tttccacgat
    42241 gattatcggc accctgccgg tggtcattcc tgtctttgcc aatctgcttt atagccagcg
    42301 cgacggcaaa ctcgcgtggg gaaaactcgc cccggcactg atttgtattg gcatcggcct
    42361 ggcgagtgtg aatattgctg agttaaacca cggactcccc gattttgact gggcacgtta
    42421 tacctctggc atcgtgctag cgttagtttc cgtggtctgc tgggcatggt atgccctgcg
    42481 caacgcccgc tggctgcggg agaatcccga caaacatccg atgatgtggg cgacggcgca
    42541 ggcgctggtc acactgccgg tttctctcat cggctatctc gtcgcctgtt actggctgaa
    42601 tacgcaaacg ccggacttct ccctaccttt tggcccccgt ccgctggtgt ttattagtct
    42661 gatggttgcg atagccgtgc tttgctcatg ggttggcgca ctctgctgga acgtcgccag
    42721 ccagcgatta ccgacagtga ttctcgggcc gctgatcgtt ttcgaaacac tggcaggttt
    42781 gctgtacacc tttttgatac gccagcaaat gccgccgctg atgacgctga gcggtatcgc
    42841 gctgttagtg gttggcgtgg tcattgcagt cagagcaaaa ccagaaaaac ccttaactga
    42901 atctgtctca gaaagttgac atactggctg tgagttaaat aagcctctgc tacgtaaggg
    42961 ttatagcttt tgccttaaag atgcatttaa aatacatctt atcttattaa gaatgaggta
    43021 tcagctatgg cttatcgcga ccaaccttta ggtgaactgg cgctctctat tcctcgcgct
    43081 tcagctctgt ttcgtaaata tgatatggat tactgctgtg gcggtaagca gacgctgtcg
    43141 cgcgcggcgg cacgtaaaga actggatgtt gaggtcattg aagctgaact ggcaaagctt
    43201 gctgaacaac cgattgagaa agactggcgt agcgccccgc tggcagaaat cattgaccat
    43261 atcatcgtgc gctaccacga tcgtcaccgc gagcagctgc cggagctgat cctgcaagcg
    43321 actaaagtcg agcgcgttca cgccgacaaa ccgagcgtgc caaaagggct gacaaaatac
    43381 ctgactatgc tgcatgaaga gctttccagc cacatgatga aagaagagca gatcctcttc
    43441 ccgatgatca aacaaggcat gggcagccag gcaatggggc caatcagcgt aatggaaagc
    43501 gagcacgatg aagcgggcga actgctggaa gtgattaaac acaccaccaa taacgtcaca
    43561 ccgccgccgg aagcctgcac cacctggaaa gcgatgtata acggtattaa tgaactgatt
    43621 gatgacctga tggatcacat cagcctggaa aacaatgtac tgttcccacg cgcgctggcg
    43681 ggtgagtaat ttgttcgatc aacgcacctg gtttacacaa tcaggtgcgt tttcaaatgc
    43741 tttgagttat tcaccactcc gggacaatct tttgtatcta ctgaactggg aaaattcatt
    43801 attcagccaa taataagttc cttcaacggt tactaactta tttttttcat taatataatt
    43861 acttattcat cgctcctgta taagccagaa taaaatcacc cattccctgg tttttacgtg
    43921 tacatccagc taccgctacg cttccaccca caccgacccg ccatcatggc ggttcatcag
    43981 cgaccacagc acatggaatt tctcgcctct tttatactcc ccctccattt tcctgtccgt
    44041 ccattctggc atctgctctc cctgctccag tgtcaccgtc gcttgtctac cttctacaaa
    44101 aatcgaccac tccccgctat gaggacaagc ttcaccgcta aaggccgcta tccgctgccg
    44161 gtctgtaaca tactgcgctt cgcgctgtgg ccagttgtac aactcctccg gcatgccctg
    44221 tgggaaaaat ccctccggcg gcacattgat aaatcggttt gccacccgcc gtatacgttt
    44281 ccaattgcat tttgcccacg ccccgctttc ccagtaatat tcttgtatcc cagcctcgtt
    44341 gacatacttc gttcgtaccc gcccctgcca agcctcggga atatgttcac tccggtacaa
    44401 cagttgggcg ctggcatctg ggatatccgg cagatacagg ccagccaccg ttaccgagtc
    44461 atcctcacgc aggtatacgc tactatccag ctcatacaac ggtaattgca tcggcagggc
    44521 cagcgggccg tgcagacttt cctcataata ataagtcagt tcgccgtcat accagtatcc
    44581 gcccgaggta cgtctgatta aattattata atgtgcacct cgccatttca gtctgtcata
    44641 ctgtttttgc gcggcgagtg acatccagtc cgacggataa tcaattagtc ctttcatatc
    44701 actgctaata ttgcccccgg attgcagtac ccgcaggtcc ccctgctgta gctgaataaa
    44761 gtcatactgt accgacttta aagtactgcg gatattgggc aacactcgcc cgccccagac
    44821 gacgtcaatc tgctcgcaca gcggacgacg gcgcacatcc agcggcatgg tcttgctcca
    44881 ctctgccacc tgtcgctcgc acagctcgac aaactcctgc cagccctcaa aacgacgccg
    44941 ataatattcc ggcgacatta ttctttccag caaccagcac tcctgtggat gtaatatagc
    45001 cactttatct cctgatttat taattaattg tctattgata cttttatttc ggtacataaa
    45061 tatttgtttg caactgagac acagctatat acatatccac ttcatctatc atcggtacgc
    45121 tgaccgtcaa aatagacgct gcgtttatcg gcagcgaaag caccgaatgc ctgaaaactg
    45181 gcggcatcga cattaggttc accgggcgga tttttcagga ctttgccaga ccaaagaaca
    45241 gagcgtccat cggtgagcca ggtatgctgg caattattcc gatcatcgca atcgaactta
    45301 tcgggattaa aatcggacag taagcgggtt tcttggttat tggctaaatt ggcaatacgc
    45361 cagaggcggg aaaaattaac gcccggcagt tcggagagga tctcatcctt ttctcccttc
    45421 tgttcggcaa cataaaccgt gcctttctcc tgctggcggt aataaaaatc tgcgccccag
    45481 gaaggtgaaa tggagagagg taatgcggcg attaataacc aactgtgctt agccatagtg
    45541 aactctgcaa tgagtggcaa agatccacgg taagcccgcg cctgagagct gtcgatgacg
    45601 ggaagtattt ttaggaatgt ataaagacaa aggagattgc tgacaaagtg tgctttgttc
    45661 atgccggatg cggcgtaaac gccttacccg gcctacaaaa gcacgcaaac tcaatatatt
    45721 gcagaggttc tgtaggcctg ataagcgtag cgcatcaggc aattttgcag cctgaaagac
    45781 acccgcaggt gccttagcgg cattacttcg ccatgcgttt tttaccaata aacagccagc
    45841 ccaaccccag cgcgataaac cacagcgggg taaccagcag cgcctggcga gtgtcatctt
    45901 ccagtgtcag cagcacgatc acgaacacaa agaacgccat acatacccag cacatcagct
    45961 tgccgagcgg catcttgtag atcgacttct catgcagatg aggacgctgt ttgcgataca
    46021 ccaggtacga gcaaaggata atcgtccaga cgaacataaa cagaatcgcg gaaaccgtcg
    46081 tgatcatcgt gaacgcgcca atcacgctgg gattcacata cagcatcacc acgccgccga
    46141 gcagacagat gcaggagaag gtcagccctt tcgcgggtac tgcgcgctta gagagtttag
    46201 cgaacgcttt cggtgcaaca ccttcctggg ccaggccaaa cagcatacgg ctggtagaga
    46261 agacgccgct gttagcggaa gacgccgcag aggtcagcac cacaaagttg atcacgctgg
    46321 cagccgcagg caaacccacc aacacgaaca gttcaacaaa cgggcttttc tccgggacta
    46381 ccgaactcca cggcgtcacg gacataatta caatcagcgc gaagacgtag aacataatga
    46441 tacggatcgg aatagagtta attgcacgcg gcagtgattt ctccggatct ttggtttccg
    46501 cagcggttgt acctaccagc tcaatcccta cgaaagcgaa aaccgctatc tggaatccgg
    46561 caaagaagcc acttaaacct ttcgggaacc agccgccatc attccacaaa tgcgcgaatg
    46621 acgcttccac accagtcggt gactgaaagt gcatcgccac cataaccagg ccgacgacaa
    46681 tcagcgacac aatggcgacg attttgatca tcgcaaacca gaactccatc tcaccgaaca
    46741 ttttcacggt ggcgagattg agcgtcagca gcagcactat caccgccagc gaggcgaccc
    46801 agtcggagag atcggggaac cagaactgag cataagccgt gatcgccacc acgtctgcca
    46861 taccggttac aacccagcag aaccagtaag tccagccggt gaaatatcct gcccacggcc
    46921 cgagtaaatc ggaagcgaag tcactaaaag atttgtattc cagattcgaa agcagcaatt
    46981 cccccattgc ccgcatcacg aaaaagagca taaaaccgat gatcatataa acgaaaatga
    47041 tcgacggccc ggcaaggcta atcgttttgc cagaccccat aaacaacccc gtaccaatgg
    47101 caccgccaat ggcaataagc tgaatatgtc ggtttgtgag attgcgccgt agcgactgtt
    47161 cagccggagc ctgatcatcg gcaacgactt ttacctgatc taccatgttt ttttcttcct
    47221 gtacctgtct gtgttgttca ggctctacgg cctttagtca gtctatgata cgacgaatct
    47281 gtatcgggac tcatcgatat taggtaacaa tacgcgggat gaatactaag atttagatct
    47341 aatgttaatt ttatgtttaa agtgagtgtc atatcacggt gataacgcga aacagctcac
    47401 aaaaatacac gcttacgggg tctttgcaac acaaaatagc gccgtttggc aaactgaagg
    47461 gtttattgct gaatgcctgc tcccctctgg ttcgagggga acagaatgcg ctgcttagag
    47521 gatttccagc agttcgactt caaacaccag ggtgctgaac ggagggatgg atgcgcctgc
    47581 gccgcgctcg ccatatgcca gttcctgcgg gatagtcagt tcccatttag aacctaccgg
    47641 catcagagtc agtgcttcaa tccagccagg gatcacgcca ttaaccggaa attcagcggg
    47701 ttcaccacga gcaacggagc tgtcaaacac ggtgccgtcg atcagtttac cggtgtaatg
    47761 aacacgaacg cggtcggtac gtgccggaat tgcgccttca ccctggttga tcacgcggaa
    47821 ttgcaggcca gattcggtgc tattcacacc ttcttttttg gcgttttctt ccaggtattt
    47881 cacaccttca gcagccatcg cctggaaacg ctgacgacga acggcatcgg cgcgctcgtg
    47941 gatttcacgc agcgcgcgat gcaccacatc aaccggaaca gccggatgtt tgccttccag
    48001 cgcatcggca atacctgcaa ccagtgcttc tggcagcagc ccttccaggc cagattcact
    48061 cagttgttgc cctacctgca aaccaatgcc gtagcttgct tgcgcttcga tggtgtcaaa
    48121 agttggggtg gtcatcgtat ttcctttctt agggtataaa agtagcgggc agcatatcag
    48181 ccacacttct ttgggtaaaa ctttgtcgca ggaatgatga caaatcccgg ggaaaaagaa
    48241 acaatagcaa tatcccgtat gaatataata aaggcgggtt taccacggac atcatagcgt
    48301 tagcttattc ataggctatg attgaggaac aagacgcgga gcaggaggaa accatgcccg
    48361 ggcgctttga actaaaacca accctggaga aagtctggca cgcgccggac aattttcgct
    48421 tcatggaccc gctgccgccg atgcatcgtc gggggatcat tattgccgcc attgtattgg
    48481 tggtcggatt tctgcttcca tctgatgata cgcccaacgc gccggtggtg acgcgtgaag
    48541 cgcagttaga tattcagtcg caatcacaac cgccgacaga agaacagctg cgggcgcaat
    48601 tagtcacccc acaaaatgat cctgatcagg tggctccggt cgctccagaa cctatacagg
    48661 aaggccagcc agaagagcag ccgcaaacta cccaaacgca accattccag ccagatagcg
    48721 gaattgataa tcaatggcgc tcttaccgtg tcgaacccgg taaaacgatg gcgcagctgt
    48781 tccgcgacca cggactgccc gctaccgatg tctatgcaat ggcgcaggta gaaggcgcgg
    48841 gtaagccgct gagtaatttg caaaacgggc aaatggtgaa aatccgccag aacgccagcg
    48901 gcgtggtgac gggcttaacc attgatacgg ggaataatca acaggtcctg tttacccgcc
    48961 agccggatgg cagttttatt cgtgcgcggt agatgaaggg tcaaccaaaa gccagctaag
    49021 gattgtctct ttgatgacct tacgtctggc ttccagtgcc tggggggaaa ccatactgat
    49081 atgataaacc aggccaagcg tatgctggtt gatcaaataa tatcccccaa gcgcagcaat
    49141 attaatattc acctgcaatg ggtcgatatc tggcttaaaa atattgtgtt tcttcccttc
    49201 atccaataag gattccatta actgcaagtg cgcatggtta atttctaaga gacgctgtga
    49261 ttttgcataa tgcacacctt tactttgatt ttcactatgc acaattttta aaaaccaggg
    49321 gttagcaata taataatccc atgtaaaatc aataattttc tcaatagctt tttgtggcgg
    49381 aagatctgag aaatcaaggg ccttttcttt ttgtcgaata ttgttccagg tatattccag
    49441 ta
//
LOCUS       DAPHJE010000019        48328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPHJE010000019 DAPHJE010000000
VERSION     DAPHJE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.019"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            161..1378
                     /gene="alaA"
                     /locus_tag="RH222_001118"
     CDS             161..1378
                     /gene="alaA"
                     /locus_tag="RH222_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV3796172.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1462..2061
                     /gene="yfbR"
                     /locus_tag="RH222_001119"
     CDS             1462..2061
                     /gene="yfbR"
                     /locus_tag="RH222_001119"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV3796173.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     ATIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2120..3952)
                     /locus_tag="RH222_001120"
     CDS             complement(2120..3952)
                     /locus_tag="RH222_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV3796174.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGGSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4039..4689)
                     /gene="hxpA"
                     /locus_tag="RH222_001121"
     CDS             complement(4039..4689)
                     /gene="hxpA"
                     /locus_tag="RH222_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV3796175.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4700..5194)
                     /locus_tag="RH222_001122"
     CDS             complement(4700..5194)
                     /locus_tag="RH222_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV3796176.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5277..5732)
                     /locus_tag="RH222_001123"
     CDS             complement(5277..5732)
                     /locus_tag="RH222_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV3796177.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6070..7272
                     /gene="ackA"
                     /locus_tag="RH222_001124"
     CDS             6070..7272
                     /gene="ackA"
                     /locus_tag="RH222_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV3796178.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7347..9491
                     /gene="pta"
                     /locus_tag="RH222_001125"
     CDS             7347..9491
                     /gene="pta"
                     /locus_tag="RH222_001125"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV3796179.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9681..11201
                     /gene="yfcC"
                     /locus_tag="RH222_001126"
     CDS             9681..11201
                     /gene="yfcC"
                     /locus_tag="RH222_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV3796180.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11234..11776)
                     /gene="yfcD"
                     /locus_tag="RH222_001127"
     CDS             complement(11234..11776)
                     /gene="yfcD"
                     /locus_tag="RH222_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV3796181.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11834..12385)
                     /gene="yfcE"
                     /locus_tag="RH222_001128"
     CDS             complement(11834..12385)
                     /gene="yfcE"
                     /locus_tag="RH222_001128"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV3796182.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12441..13085)
                     /gene="yfcF"
                     /locus_tag="RH222_001129"
     CDS             complement(12441..13085)
                     /gene="yfcF"
                     /locus_tag="RH222_001129"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV3796183.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13221..13868
                     /gene="yfcG"
                     /locus_tag="RH222_001130"
     CDS             13221..13868
                     /gene="yfcG"
                     /locus_tag="RH222_001130"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV3796184.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13925..14287
                     /gene="folX"
                     /locus_tag="RH222_001131"
     CDS             13925..14287
                     /gene="folX"
                     /locus_tag="RH222_001131"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV3796185.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14308..15201
                     /locus_tag="RH222_001132"
     CDS             14308..15201
                     /locus_tag="RH222_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV3796186.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15249..16139)
                     /gene="rpnB"
                     /locus_tag="RH222_001133"
     CDS             complement(15249..16139)
                     /gene="rpnB"
                     /locus_tag="RH222_001133"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV3796187.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16336..17109)
                     /gene="hisP"
                     /locus_tag="RH222_001134"
     CDS             complement(16336..17109)
                     /gene="hisP"
                     /locus_tag="RH222_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV3796188.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17117..17833)
                     /locus_tag="RH222_001135"
     CDS             complement(17117..17833)
                     /locus_tag="RH222_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3796189.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17830..18516)
                     /gene="hisQ"
                     /locus_tag="RH222_001136"
     CDS             complement(17830..18516)
                     /gene="hisQ"
                     /locus_tag="RH222_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV3796190.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18606..19388)
                     /gene="hisJ"
                     /locus_tag="RH222_001137"
     CDS             complement(18606..19388)
                     /gene="hisJ"
                     /locus_tag="RH222_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV3796191.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19609..20391)
                     /gene="argT"
                     /locus_tag="RH222_001138"
     CDS             complement(19609..20391)
                     /gene="argT"
                     /locus_tag="RH222_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV3796192.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20657..21226)
                     /gene="ubiX"
                     /locus_tag="RH222_001139"
     CDS             complement(20657..21226)
                     /gene="ubiX"
                     /locus_tag="RH222_001139"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV3796193.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21321..22838)
                     /gene="purF"
                     /locus_tag="RH222_001140"
     CDS             complement(21321..22838)
                     /gene="purF"
                     /locus_tag="RH222_001140"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV3796194.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22875..23363)
                     /gene="cvpA"
                     /locus_tag="RH222_001141"
     CDS             complement(22875..23363)
                     /gene="cvpA"
                     /locus_tag="RH222_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV3796195.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23622..24284)
                     /gene="dedD"
                     /locus_tag="RH222_001142"
     CDS             complement(23622..24284)
                     /gene="dedD"
                     /locus_tag="RH222_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV3796196.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24274..25542)
                     /gene="folC"
                     /locus_tag="RH222_001143"
     CDS             complement(24274..25542)
                     /gene="folC"
                     /locus_tag="RH222_001143"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV3796197.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25612..26526)
                     /gene="accD"
                     /locus_tag="RH222_001144"
     CDS             complement(25612..26526)
                     /gene="accD"
                     /locus_tag="RH222_001144"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV3796198.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDHCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26682..27341)
                     /locus_tag="RH222_001145"
     CDS             complement(26682..27341)
                     /locus_tag="RH222_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3796199.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27424..28236)
                     /gene="truA"
                     /locus_tag="RH222_001146"
     CDS             complement(27424..28236)
                     /gene="truA"
                     /locus_tag="RH222_001146"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV3796200.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28236..29249)
                     /locus_tag="RH222_001147"
     CDS             complement(28236..29249)
                     /locus_tag="RH222_001147"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV3796201.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29315..30451)
                     /gene="pdxB"
                     /locus_tag="RH222_001148"
     CDS             complement(29315..30451)
                     /gene="pdxB"
                     /locus_tag="RH222_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV3796202.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30550..31545
                     /gene="flk"
                     /locus_tag="RH222_001149"
     CDS             30550..31545
                     /gene="flk"
                     /locus_tag="RH222_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV3796203.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31542..32720)
                     /locus_tag="RH222_001150"
     CDS             complement(31542..32720)
                     /locus_tag="RH222_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDV3796204.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33004..34224)
                     /gene="fabB"
                     /locus_tag="RH222_001151"
     CDS             complement(33004..34224)
                     /gene="fabB"
                     /locus_tag="RH222_001151"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV3796205.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34383..36389
                     /gene="mnmC"
                     /locus_tag="RH222_001152"
     CDS             34383..36389
                     /gene="mnmC"
                     /locus_tag="RH222_001152"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV3796206.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTIPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36510..36788)
                     /locus_tag="RH222_001153"
     CDS             complement(36510..36788)
                     /locus_tag="RH222_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV3796207.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36822..37370)
                     /gene="epmC"
                     /locus_tag="RH222_001154"
     CDS             complement(36822..37370)
                     /gene="epmC"
                     /locus_tag="RH222_001154"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV3796208.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37370..38179)
                     /locus_tag="RH222_001155"
     CDS             complement(37370..38179)
                     /locus_tag="RH222_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV3796209.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38179..39003)
                     /gene="mepA"
                     /locus_tag="RH222_001156"
     CDS             complement(38179..39003)
                     /gene="mepA"
                     /locus_tag="RH222_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV3796210.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39007..40092)
                     /gene="aroC"
                     /locus_tag="RH222_001157"
     CDS             complement(39007..40092)
                     /gene="aroC"
                     /locus_tag="RH222_001157"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV3796211.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40127..41059)
                     /gene="prmB"
                     /locus_tag="RH222_001158"
     CDS             complement(40127..41059)
                     /gene="prmB"
                     /locus_tag="RH222_001158"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV3796212.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41225..41776
                     /gene="smrB"
                     /locus_tag="RH222_001159"
     CDS             41225..41776
                     /gene="smrB"
                     /locus_tag="RH222_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV3796213.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41898..42770)
                     /locus_tag="RH222_001160"
     CDS             complement(41898..42770)
                     /locus_tag="RH222_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV3796214.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42757..43281)
                     /locus_tag="RH222_001161"
     CDS             complement(42757..43281)
                     /locus_tag="RH222_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3796215.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43278..43748)
                     /locus_tag="RH222_001162"
     CDS             complement(43278..43748)
                     /locus_tag="RH222_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3796216.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQVGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43745..44293)
                     /locus_tag="RH222_001163"
     CDS             complement(43745..44293)
                     /locus_tag="RH222_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3796217.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44268..45020)
                     /locus_tag="RH222_001164"
     CDS             complement(44268..45020)
                     /locus_tag="RH222_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3796218.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45040..47685)
                     /gene="yfcU"
                     /locus_tag="RH222_001165"
     CDS             complement(45040..47685)
                     /gene="yfcU"
                     /locus_tag="RH222_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDV3796219.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEEFSGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(47767..>48328)
                     /locus_tag="RH222_001166"
     CDS             complement(47767..>48328)
                     /locus_tag="RH222_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3796220.1"
                     /translation="SKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSI
                     VPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANS
                     GNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFK
                     AWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11935 a        13025 c        11980 g        11388 t
ORIGIN      
        1 ttattttgtc attattaagt tattaacagc acaatcgagc cttcccctct ggcaaaatct
       61 tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag ctgacttcca
      121 cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc
      181 cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg
      241 cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag ccccgttcgg
      301 ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta ccgctcaagg
      361 gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc atcatgcagc actaccaggc
      421 tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg tatcggagct
      481 tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg ttcctgcacc
      541 agattaccca ctatggaccg cggcggtttc gctttccagc ggtaaagcgg tgcattatct
      601 ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta aaattacgcc
      661 tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca accggcgcgg tatattccaa
      721 agagctttta atggagattg tggagattgc acgtcagcat aatctcatta tcttcgccga
      781 tgaaatttat gacaaaattc tttacgacga cgctgagcat cactcaattg cgccgctggc
      841 acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt
      901 ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg
      961 tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca
     1021 gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg gtcgtcttta
     1081 tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt cctgcgtgaa
     1141 acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta acattcacga
     1201 cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac
     1261 ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc gtgtcgatga
     1321 tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc agctgtaatc
     1381 ttaatttcac tgccggagat tgcatccggc agcgttatcc cgccacaatg acctgatgat
     1441 gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct ctcccgcctg
     1501 aaactcatta accgctggcc gctcatgcgc aacgtgcgga cggaaaatgt gtccgaacac
     1561 agtttgcagg tagcgatggt cgcccatgcg ctggcaacta tcaaaaatcg gaaatttggc
     1621 ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc ctcagaagtg
     1681 ctcaccggcg atctccctac tccggtgaaa tacttcaatt cgcaaatcgc tcaggagtac
     1741 aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga agagctgcgg
     1801 gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa atcgctggtg
     1861 aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc ggccggaaat
     1921 aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg tcgcagccag
     1981 gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct cgatgagatt
     2041 agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa taagcgccgg
     2101 atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc acacaaaccg
     2161 ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag ctgtaatttc
     2221 ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg tgtcataaag gcggcggatg
     2281 ctgccatcgc cacgaccatc gcgaatggat aaggcgaccc ccccatcgtt ttggcagcag
     2341 ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaat agcccaataa
     2401 ccgccgacaa gacaaacaaa caacccagca tcatatgtgg cccgtaacca ccgccaatgt
     2461 ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg
     2521 gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag gcgctttcag
     2581 catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca acaggattag
     2641 gaatttcatc tgtcagcatt aacgccacca ttagcaccag acagaaaatg gcatggggtg
     2701 cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc gctacgaagt
     2761 cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc aggatgatat
     2821 cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca ttgcgcttta
     2881 gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa
     2941 tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg gcctggtcag
     3001 aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa aattgccgga
     3061 gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa
     3121 ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt
     3181 tagcgccata acgctcacgt aatttgagat catccagccg ttgacctatc attggcgatc
     3241 cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc
     3301 gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct ttcagcatga
     3361 aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca ataggtgtta
     3421 cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg actaccaggt
     3481 tcggcggcgt cgccaccagc gtcatcatgc cgcttatcag cccggcaaaa ctcaacggca
     3541 tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc acgggaataa
     3601 agatagcgac aacgccggtt gaactcataa acgcgccaag ccccgcgacg gtcagcatca
     3661 acaaaaccaa cattttgatt tcactgttgc ccgcaacttt gaccagccat gttcccatta
     3721 cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg gcaatcagga
     3781 caacgttagg atcagaaaag ccggaaaata cttcggggac tgtcagcgtt ccgcttaatg
     3841 caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc gtcgcaaaca
     3901 agacaatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc
     3961 cttgttaatt gagggagatg tcttgattct gccataaaaa agccccgacg agacggggct
     4021 aaatcatgat caggtgtttc actgaataat aacatcgcca tttggctgtt tggtcacagt
     4081 aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg tatctgccgg
     4141 agcgttaacc gcaatgacat ggcaacccgc cgccaggcca gaaagcacgc cagcgggagc
     4201 atcttccacc accacacact cctgcggcgc aagccccagc agctgcgcac ctaacagata
     4261 cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc
     4321 gggaagccca gctattttat ggcgcgctcg cgctaccggc atggagccag aagtcacaat
     4381 ggcccacgga atacctgctt tattcaaatg actgagtaag gcgattgccc ccggaagcgc
     4441 ggtaatacct tcggtttccg tggcctcgat ttgctccaga cgcgtaaact cggcggcaat
     4501 atcagcctcg gatttgcccg ccataaaatg gcgcagagag gtgatcgcct gtttaccgtg
     4561 aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt tgctccacgc
     4621 ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaaa gaaaaccttt
     4681 gcaccgcacg cgggcctcct caggcgttga taatttgatt aatttcgttg gcgctcaaat
     4741 ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt tcccacattg
     4801 gcgtctgggc gttaaaaccg tgagttccgg cgtcaaaatg ggtatagcgc ccttccacat
     4861 taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga
     4921 aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag gaaacatgca
     4981 acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc
     5041 caaactcgcg atccaattca cgcatctgta atccgtaacc acgctcaata attgtttgca
     5101 ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg tactgattag
     5161 acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc actatactac
     5221 ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg ggtcaattac
     5281 aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat
     5341 gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc ctgcaattta
     5401 ccgcgaactt cataaaacca gttaaggatt gcagggggta atggcgttac agaacgcttg
     5461 cccagccacc acaatccctg catgggtaaa cttaaggcga acagcgcagt ggcaactgcc
     5521 ggcccaagct gaccgcccag ggcaatctgc cagcagagag taaatacggc gatcggcggc
     5581 ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa gaccggggca
     5641 aggcgttttt ccagtggcca cgtctttgag taatgctgtc cgcggcgaaa caagctaaaa
     5701 aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac atataaagat
     5761 tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt ttgtcttcca
     5821 tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata ttcattgagt
     5881 cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc atagactcaa
     5941 gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg
     6001 tgtcatcatg cgctacgctc tatggctcac tgacgttttt ttagccacgt atcaattata
     6061 ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc ttcactgaaa
     6121 tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc
     6181 cacctgcccg aagcacgtat caaatggaaa atggacggta ataaacagga agcggcttta
     6241 ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat tctggcacaa
     6301 aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca cggcggcgaa
     6361 aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa agatgcagct
     6421 tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc tctgaaatct
     6481 ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca ccagactatg
     6541 ccggaagagt cttacctcta cgccctgcct tacaacctgt acaaagagca cggcatccgt
     6601 cgttacggcg cgcacggcac cagccacttc tatgtaaccc aggaagcggc aaaaatgctg
     6661 aacaaaccgg tagaagaact gaacatcatc acctgccacc tgggcaacgg tggttccgtt
     6721 tctgctatcc gcaacggtaa atgcgttgac acctctatgg gcctgacccc gctggaaggt
     6781 ctggtcatgg gtacccgttc tggtgatatc gatccggcga tcatcttcca cctgcacgac
     6841 accctgggca tgagcgttga cgcaatcaac aaactgctga ccaaagagtc tggcctgctg
     6901 ggtctgaccg aagtgaccag cgactgccgc tatgttgaag acaactacgc gacgaaagaa
     6961 gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg cgaaatacat cggtgcctac
     7021 actgcgctga tggatggtcg tctggacgct gttgtattca ctggtggtat cggtgaaaat
     7081 gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg tgctgggctt tgaagttgat
     7141 catgaacgca acctggctgc acgtttcggc aaatctggtt tcatcaacaa agaaggtacc
     7201 cgtcctgcgg tggttatccc aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc
     7261 ctgactgcct gatttcacac cgccagctca gctggcggtg ctgttttgta acccgccaaa
     7321 tcggcggtaa cgaaagagga taaaccgtgt cccgtattat tatgctgatc cctaccggaa
     7381 ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg
     7441 ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac cggtggcgat gcgcccgatc
     7501 agactacgac tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa
     7561 tgagctacgt tgaaggtctg ctttccagca atcagaaaga tgtgctgatg gaagagatcg
     7621 tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc
     7681 cgacacgtaa gcaccagttt gcccagtctc tgaactacga aatcgctaaa acgctgaatg
     7741 cggaaatcgt cttcgttatg tctcagggca ctgacacccc ggaacagctg aaagagcgta
     7801 tcgaactgac ccgcaacagc ttcggcggtg ccaaaaacac caacatcacc ggcgttatcg
     7861 ttaacaaact gaacgcaccg gttgatgaac agggtcgtac tcgcccggat ctgtccgaga
     7921 ttttcgacga ctcttccaaa gctaaagtaa acaatgttga tccggcgaag ctgcaagaat
     7981 ccagcccgct gccggttctc ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg
     8041 cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc
     8101 gccgcgttaa atccgtcact ttctgcgcac gcagcattcc gcacatgctg gagcacttcc
     8161 gtgccggttc tctgctggtg acttccgcag accgtcctga cgtgctggtg gccgcttgcc
     8221 tggcagccat gaacggcgta gaaatcggtg ccctgctgct gactggcggc tacgaaatgg
     8281 acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg
     8341 tgaacaccaa cacctggcag acctctctga gcctgcagag cttcaacctg gaagttccgg
     8401 ttgacgatca cgaacgtatc gagaaagttc aggaatacgt tgctaactac atcaacgctg
     8461 actggatcga atctctgact gccacttctg agcgcagccg tcgtctgtct ccgcctgcgt
     8521 tccgttatca gctgactgaa cttgcgcgca aagcgggcaa acgtatcgta ctgccggaag
     8581 gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt
     8641 gcgtactgct gggtaatccg gcagagatca accgtgttgc agcgtctcag ggtgtagaac
     8701 tgggtgcagg gattgaaatc gttgatccag aagtggttcg cgaaagctat gttggtcgtc
     8761 tggtcgaact gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag
     8821 acaacgtggt gctcggtacg ctgatgctgg aacaggatga agttgatggt ctggtttccg
     8881 gtgctgttca cactaccgca aacaccatcc gtccgccgct gcagctgatc aaaactgcac
     8941 cgggcagctc cctggtatct tccgtgttct tcatgctgct gccggaacag gtttacgttt
     9001 acggtgactg tgcgatcaac ccggatccga ccgctgaaca gctggcagaa atcgcgattc
     9061 agtccgctga ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg ctctcctact
     9121 ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg
     9181 cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct gcagtacgac gctgcggtaa
     9241 tggctgacgt tgcgaaatcc aaagcgccga actctccggt tgcaggtcgc gctaccgtgt
     9301 tcatcttccc ggatctgaac accggtaaca ccacctacaa agcggtacag cgttctgccg
     9361 acctgatctc catcgggccg atgctgcagg gtatgcgcaa gccggttaac gacctgtccc
     9421 gtggcgcact ggttgacgat atcgtctaca ccatcgcgct gactgcgatt cagtctgcac
     9481 agcagcagta atctcgtcat catccgcagc tttgcgctgc ggatatctga accggaaata
     9541 atcactattt ccggtttttt attctcttaa tctgcattaa tcctttctga ttatcttgct
     9601 taactgcgct gcatcaatga attgcgccat ttcactttgc atacttacca ctttgttttg
     9661 tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc caaaccaaca agaagatggg
     9721 caatgcccga tacgttggtg attatctttt ttgttgctat tttaaccagc cttgccacct
     9781 gggtagttcc ggtggggatg tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa
     9841 caaaaacacg caaagtcgta gatccacact catttcgcat tctgactaac gaagcaggcg
     9901 aacctgagta tcaccgcgta cagctgttca cgacgggcga tgaacgcccg ggcctgatga
     9961 acttcccgtt tgaaggatta acctcaggat cgaaatacgg gacagctgtt ggcatcatca
    10021 tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga accattgata
    10081 acggtatcct ggcgcttatt cgccataccc gcgggaatga aattctcttt attcctgcgc
    10141 tgtttattct gttttcactt ggcggcgcgg tatttggtat gggagaagag gccgtcgcct
    10201 ttgccattat catcgcaccg ctaatggtcc ggctgggcta tgacagtatt accaccgtcc
    10261 tggtgaccta tattgccacg caaatcggtt ttgccagttc gtggatgaac ccgttttgtg
    10321 tggtcgttgc tcaggggatt gccggcgttc cggtgctttc tggctccggg ttgcgcatcg
    10381 tggtgtgggt tatcgccact ctgattggcc tgatctttac catggtgtac gcctcacgag
    10441 tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc
    10501 aggctgatgt tgaacaacgt ccgtttacct ttggtgactg gctggtattg attgtcctga
    10561 ccgccgtaat ggtctgggtg atttggggcg tgatcgttaa tgcctggttt attccagaaa
    10621 ttgccagcca gttcttcacc atgggtctgg tgattggcat catcggcgtt gttttccgcc
    10681 ttaacggcat gacggttaat accatggctt catcctttac cgaaggggcg cgaatgatga
    10741 tcgcccctgc cctgctggtg ggtttcgcca aagggatttt gctgctggtc ggtaatggtg
    10801 aagcaggtga tgccagcgtg ttaaatacca tcctcaacag cattgccaat gccattagcg
    10861 gtctggacaa cgctgtcgcg gcctggttta tgttgctctt ccaggcggta tttaatttct
    10921 ttgtgacatc cggttctggt caggcggcgt taaccatgcc gttactggca ccgcttggcg
    10981 atctggtcgg tgttaaccgt caggttaccg tgctggcatt ccagtttggt gatggcttca
    11041 gccacatcat ttacccaacc tcagcttcgt taatggcgac gctcggtgtt tgccgggtgg
    11101 acttccgtaa ctggctgaag gtgggcgcga cactgcttgg actgctgttt attatgtcca
    11161 gcgtcgtggt gatcggcgct cagttgatgg gctaccacta aaaatgttaa gagccgcaat
    11221 tgcggctctt ttttcattct gccgtttcag tctctacggc ttcatttttg gcattgcgct
    11281 tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga
    11341 tttcttccgg cgtcagccag caaacttcac tgacttcatc ttcctgtagg gcgaagggac
    11401 cgtgagagac gcagctgaac aatgcgcccc agacacggca atttttatct tcgaaataga
    11461 actgcccgtg ctcagcaaag gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac
    11521 gcgcggattc cagcagttgc tcatcggcct ggaccacacc gcctgcggtc gcatctaaca
    11581 tgccgggtaa aaagtctttt gtctcggtac gacgctggac cagaattttg cccatgccat
    11641 catgcacgac gatgtaagtt gcacgatgac gcagacactg tgcccgcatt tgttcccggc
    11701 tggcttgtgc aatgacttcg ttctcttcat tgacaatatc cacccattca gtacttgcca
    11761 aacgacgctg ttccattatc aggaaacctt ctttttctgg cgctcttacg gcgcattttg
    11821 agttgtgggt aaattacgga ttaatcgcga cctgcgcaat gatactttga tcattgagtg
    11881 cgattacact aagtacgtca ttatccagca tgccataact cgccggatta ccgcctttcg
    11941 gaatactcac cgagccgggg ttgaagtgga aaatctcccc ccgctgttct gccactggca
    12001 gatgggtatg accgtacacc agcacatcgt tctggtttaa agcaggtaga ttttccgggc
    12061 caaaaagatg accatgcgtc aaaaacagac gttgtttttc cagtaatacc tgttgccacg
    12121 gcgcggttat cgggaaatgc agcagcattt gatccacttc gctgtcgcag ttgccgcgca
    12181 cagcgataac cttatgtgcc acttcattaa gccgttcagc gactttggct ggcgcgtaac
    12241 cctccggtaa agcattacgc gggccgtgat tcaacacgtc gccaaggatc accagccact
    12301 gggcaccgct ttgggcaaac aactccagaa cacgttccgt cgccggtaac gacccatgaa
    12361 tgtccgatgc aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt aagctatgat
    12421 actggattct gtcgttgcta tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg
    12481 acagacgctc gctgccactg gaatgtcgca taatccacca ggcgttctgg cacctcatcg
    12541 ccatgtagta ccaggcggtt aatcattagc gccagatcag tatcagcaat gcaccattca
    12601 ccaaataaat tcggctgacc cagtgctaac aaatgttctg ccatcgcgaa cagtttctct
    12661 gcactggctt ttccctcggc cgttagtggc gcttttttcg cccccgcaaa gacaacatcc
    12721 gtcggacgct cttcgcggat gggcatcaga tcgctgcgca gccaggcctg aatctgtcgt
    12781 gcacgcgcac gattttctaa atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat
    12841 cgatcttcca gatactccgc aatggcagaa gattcactca actcaaaatc atcgatttgt
    12901 aataacggca cacggcgtgt ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg
    12961 ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt tttcctgcaa cgccacccag
    13021 gcggataaca cataagggga gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta
    13081 ctcattatgt cctcggcaat aaagggggct ggctatcaaa ctatagagcc tttttgtcac
    13141 tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg
    13201 attaaacaac cggagctgca atgatcgatc tctatttcgc cccgacaccc aatggtcaca
    13261 aaattacgct gtttctcgaa gaagcagagc ttgattatcg cttgattaag gtagacctgg
    13321 ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc gcccaacaac aaaattccgg
    13381 caattgttga tcattctcct gccgatggcg gcgaaccgtt aagcctcttt gaggctggtg
    13441 ccattttgtt gtatctggct gagaaaacag gactcttttt gagtcatgaa acgcgtgagc
    13501 gcgccgccac attacagtgg ttattctggc aggtaggcgg actggggccg atgcttgggc
    13561 aaaatcatca ttttaatcac gcagcccccc aaaccattcc ttacgctatt gaacgttatc
    13621 aggttgaaac tcagcgtctt taccatgtac tgaacaagcg gctggaaaac tcgccctggc
    13681 tgggaggcga gaactacagc attgcggata ttgcctgctg gccgtgggtt aatgcctgga
    13741 ctcgccagcg aattgacctc gcaatgtatc cggcagtcaa gaactggcat gagcggatcc
    13801 gttcgcgccc tgccaccggg caggcactgc taaaagcaca actcggtgat gagcgttcgg
    13861 atagttaaca gaaacaggtt ctcgtgtatt atttcatcct aagtaaaaca acggagaacc
    13921 tgcaatggca caacctgccg ctattattcg tataaagaac cttcgtttac gtacgtttat
    13981 cggaattaag gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg tgacgatcca
    14041 ctaccccgcc gataaagcgc gcactagcga agatatcaac gatgcgctga attatcgcac
    14101 cgtaacgaaa aacattattc agcatgtaga gaataaccgt ttctctttgt tggaaaaatt
    14161 aactcaggat gtgctcgata tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga
    14221 gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta tccatgacct taagctggca
    14281 gcgttaaccg ccatatcggg aggctgcatg aacatagtga tcaccggagg gacgggatta
    14341 attggtcgcc atttgattcc gcgtttgctg gagctgggcc atcaaatcac ggtagtgacg
    14401 cgtaacccac agaaagccag ttccgttctc ggccctcggg tgacgctatg gcaagggcta
    14461 gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca acctggccgg agaaccgatt
    14521 gctgataaac gctggactca cgagcaaaaa gagcgtctct gccaaagccg ctggaatatc
    14581 acgcaaaaac tggtcgattt gattaatgcc agcgacacgc cgccgtcggt actcatttcc
    14641 ggctcggcaa cgggctatta tggcgactta ggtgaagtgg tggttaccga agaggaaccg
    14701 ccgcataacg aatttactca taaactctgc gcccgctggg aagagattgc ctgccgggcg
    14761 caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg tggtactggc accggatggc
    14821 ggtattctcg gtaaaatgct gccgccgttt cgtcttggcc tgggcgggcc gattggctct
    14881 ggtcggcagt atctggcctg gattcatatt gatgatatgg tcaacggtat tctctggctg
    14941 ctggataacg agctgcgcgg gccatttaat atggtttcgc cctacccggt acgcaatgaa
    15001 caatttgccc atgcgctcgg tcatgcgctg catcgcccgg ccattttgcg cgtccccgcg
    15061 accgccattc gactgttaat gggcgaatct tcagtactgg tattaggtgg acaacgcgcg
    15121 ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct ggtacgattt agaagaggcg
    15181 ctggcggatg tcgttcgctg atgtggttta cagcaaacat ccgccagtta actcccggtg
    15241 ttacaggatt agtggctttg cgctataaga tcgtctggcg atagtcgggt caccatcata
    15301 actaactctc tgtctaaacc tctatccagc atctcctgag caatacgcag ggcttcttcg
    15361 tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag caatggtcat cagtttctcc
    15421 ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg cacggaaacg ctgggcatcc
    15481 cctgtttgta atacgtaatt aaacagggct tttagctgtc tgtcattagt gttccctgta
    15541 actagcagcg aaacaatttg gtcgactaat cccaacagat cgcgctgacg aatatgtttc
    15601 tgaattaact ccaacagcgc cattttgcgg tgttgcataa tctcgtcatc cggcaccacg
    15661 gtaatatcca ccaacggaaa cgctgatgaa tatattttgc gggctatagc aggctcggca
    15721 aattcatcaa gccagcagag tgaataagga taaggacttc tgcaaccatg ataaaacagc
    15781 atcgggatca ccaatggaag ctctttatag cccgcatcaa gatggttttg cattgccgca
    15841 atggaataac gcatcatgcg aaaagccatt aattcttccg gcttactttg gtgctctatc
    15901 actacataaa tataacccac tccctcctgc gttttcacag accacaagag gtcggaataa
    15961 tattgccgca ggtcgtcatc aataaaactg tttggttcca gtttaagcgt cgttaaatca
    16021 cacagtttgc gcagcggcgc gggaagatga atatcaataa aatcccgcgc ggtgtctgga
    16081 tggcgtaaaa aagatttaaa taccgcatca tgcggcgtgg aagttgtcga tattgtcatg
    16141 gcgttccgtc acccctcaaa tttaagatga cgcgacaata accacgatta acacccaatg
    16201 cagcaggcag tttttatctt tggacagcga attcagaagt atttttagcg gactgcaact
    16261 tttacatttc agcggaagct gactcgcaaa ttccccatga aaatactccg gcagcgttag
    16321 ccaccggaga ggaaattatt tcagcgatcc cttaaggaac tgttgcagac gagggctttg
    16381 cgggttgcca aataactgct ccggcgcgcc ctcttcttct attttcccct gatggaggaa
    16441 aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt
    16501 tttcccctct tctgccagtt gctgcataat acgtaacact tcgcctacca gttcaggatc
    16561 gagcgccgag gtaggttcat caaacagtaa aacttccggt tccatcgcca gcgcccgcgc
    16621 gatagaaaca cgctgttgct gaccgccgga aagatgcacc ggatatttcc cctgcgcacg
    16681 ttcgtctatc ccgactttcg ccagatactt caccgcccgc tcgcgcgctt cctgcttgct
    16741 caggcccaac acctgaatcg gcgcttccat gacgttttcc agcaccgtca tatggctcca
    16801 gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt
    16861 atcggcgact ttgagttgac catctttgtc gcgcaccaga ttgatcgtct ggccactgac
    16921 cacgatcgat ccttcgctcg gtttttcgag gaagttaatg cagcgcagaa aggtactttt
    16981 ccccgatccc gacgatccga tgatgcttat tacatctccg gcattcgctt gcagtgacac
    17041 ccctttcagc acttcgtgtt cgccatagcg tttgtgcaaa tcgataacgt ttaatttatt
    17101 ctcggacatc gtattctcag tgcgttgaag aaggtttcac atgctgcaac cagcgttttt
    17161 ccgctctgcg aaagaggctg atcaggacat aagagatgat taaatagagc accgcggcaa
    17221 tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat atcgcgggct attttcagca
    17281 gatccggcac cgtggccgta aacgccagcg cagtagagtg cagcatcagg atcacttcat
    17341 tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa aataatgcag cgatacattt
    17401 taaaggtcga gaagccatag gctctggcgg cttcaatttc cccatgcgga accgaacgga
    17461 ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg
    17521 tacagttcag gccactgcgg aagaaagcgt taaggaattc ggttccctta acaatctcaa
    17581 gcgtgtacat gccggaatag aacaccagca actgaacata cagcggcgta ccgcgaaaaa
    17641 tataggtaaa taaccagatt ggaaactgga tgtatttatt actggagaca cgaccaatcg
    17701 ccagaaacag cgccaggact ccgcctatca ctaccgacaa aataagcagc cacagagtga
    17761 tcgccacacc agtaaagcga taaccgtcgg tccacagcag cggtttccag tattcatgta
    17821 agatttcgat cacaggtcag ccctcttcac acccacggag tagcggcgct caaggaacag
    17881 cagcacacca ttggaaacag tggtgaaaac caggtaaatc acgccacaga cgatggcgaa
    17941 atagaacggt tcccaggtac ttttgcctgc cagttgggtg gctttgacca catcttccag
    18001 gccgagtaac gaaaccagtg cggtagattt gaggatcacc tgccagttgt tgccaatgcc
    18061 tggcagggcg taacgcatca tcgccggaaa catgatccgc cgaaacactt gcccacgagt
    18121 aaaaccgaac gccgtcgccg cctctatatg tcctttcggc actgccataa aagcaccacg
    18181 gaacgtttcg gtaaaataag caccgtagat aaaaccgaga gtgataatac cagcgaccat
    18241 cggatcgata tcaatctgcc cgacgcccat cgcctccgtc accgtgttta gcgcaatctg
    18301 caaaccgtag aaaatcagca gcatcaacac taagtccggc acgccacgaa tcagcgtggt
    18361 gtacccttcg aaaatcagcc ccgaaagccg attttgcgag agcttaccgc cagcgccaat
    18421 taaaccgatg attacagcga gcactacaga gctgatagcc agctccagcg tgacgagcgc
    18481 accctgtaaa ataacacctg aaaacccata caacatgctg cctgtcctgt cgtgtgtggt
    18541 gaattaccgc ctcttgtctc cctccggtta cccgaaggga gcgagctttc tttcaccgat
    18601 ggcgattaac cgccataaac atcaaaatcg aagtactttt tcgctaattt ctcgtaagta
    18661 ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct cgcgcagttc gttatcttct
    18721 ttacgcaggc ccatgccggt ccctacacca aacagttttt catctttaac agacgggcca
    18781 ccgaatttgt aatctttacc gacgggttgt ttgaggaaac cttcactggc agcgacctca
    18841 tcctggaacg cggcatcaat acgtccggca gtcaggtcag aataaatgtt gtcctgcccc
    18901 tgatacgaga cgatttcaat gccttttggt gcccagtgtt cgttaccgaa cgtctcctgg
    18961 gtggtgccct gcaatacgcc tacccgcttg cctttcagcg actcgactgt cggctgaatg
    19021 tcggaatttt tcgccaccac cagacgagaa tcggcagcgt acagtttgtc ggtgaaggca
    19081 atttcttgct gacgtttttc cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc
    19141 gcttttaagg acgggattaa cgcatccagc ggattttcga caaacgtaca ttgcgtattg
    19201 atgcgtttgc ataattcctt tgccagatcg atatcgaagc caaccagttc gccttgtgaa
    19261 ttttttgatt caaatggcgc ataggtcggg tcggtaccga tgcggatgtt ttgcggaatc
    19321 gcagcaaatg ccgcagttgc gctggagaag gccagaacca gagagagcga tagcaccagt
    19381 tttttcataa tgtcctcaac tgacagtctt tttataggga atatttacag gtttgttaca
    19441 cttatcgtgc cataaaattg accatcaagg caaaatatcc cgccccgtta gacgtattat
    19501 ttttcattaa aaatgcttaa gtatgcattt caatgcacca cgatagtgca acaaacctgg
    19561 cgtgcaccat gatgatcatt ccatcaggta cagcttccca gcgacgtatc agtcaccgta
    19621 gacattaaag tcgaaatact ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc
    19681 gccaagcgcc ttattgaagg cagccgtcag ttcagcatca tctttacgta gccctacacc
    19741 ggtgccatca ccgaagtatt ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt
    19801 accagcaggt tgcttgagga atccttcgct ggcagcaact tcatcttgta acgcagcatc
    19861 cagacgtcct gcagccagat cggaatagac caaatcctgg ttggcatagg ccaccacatc
    19921 cacgcctttg ctacgccagg tctcgttagc gtaagcttcc tgggttgatc cctgcagcac
    19981 accaacatgt ttacctttca gtgaatccag cgttggctga atcggtgaac ctttggcagc
    20041 aatcaaacga gaatctgcgg cgtacagctt gtcggagaag gcaatctcct gctgacgttt
    20101 atcggtaatg gaaagcgacg aaataatggc gtcgattttt ttcgctttca gtgaggggat
    20161 cagcgcgtca aagtcactgg caacccaggt acatttcacc tgcatccgtt tgcacatctc
    20221 gttaccgaga tcgatatcaa agccaacaaa atcaccttta gcatctttcg acgagaacgg
    20281 cgcataggtg gtatcggttc cgatacgtac cgtctccggt agcgccgcat agctggaagc
    20341 cgctgcggag agaccgacta acaaagacag agcgagaatc gacttcttca tacataaccc
    20401 tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt gtgtttgcag gctctttcat
    20461 gcaggtctta tgccatcttg acgacaacag taacattcaa cgttaaatat gttaataaga
    20521 cgttgcatta ttgtcctgaa gttgaagata gcaggtatag cggttgaatc gcagcgtttc
    20581 gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa
    20641 cagggcaaca gcggagttat gcgccctgcc agcgggcaaa gagatcttca ggaagggtta
    20701 tcgcaaactg gtcaagaaca cgattaaccg tctgatttat cacatcatca agggattgcg
    20761 ggcgatgata aaacgccgga acgggaggca taatcaccgc accgatttct gccgcctgag
    20821 tcattaaacg cagatggcct aagtgcaatg gtgtttcacg cacgcagagc accaacgggc
    20881 gacgctcttt cagcaccaca tctgccgcac gggtcagtaa gccatcagta tagctatgga
    20941 caatgccgga aagggttttg attgaacagg gtaaaatcac catccctagc gtctggaaag
    21001 aaccggaaga gatgctggcg gcaatatcgc gcgcatcgtg cgtgacatcg gctaatgcct
    21061 gcacttcgcg cagagaaaaa tccgtttcga gggataaggt ctggcgcgct gcctggctca
    21121 tcaccagatg cgtttcgata tctgtgacat cgcgcagaac ctgtaataag cgcacgccat
    21181 aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg tttcataaat agttgccctg
    21241 tcagacttgc aggcagactt tgcaggattt cggcgggagt tgcaagtcag ggtgccagac
    21301 cggcaccctc agcgaaggca tcatccttcg ttatgcattt cgagattttc cacttcgttc
    21361 tgacgttgca ctgctttggc gtcatcatta cgtaacgtat cgaggaaatc gaggtagccc
    21421 tgatcaacat ctttggtgac gtagacgccg ttgaacaccg agcattcaaa ctgctggata
    21481 tccggatttt cagcgcgaac ggcgtcgatc agatcgttca gatcctggaa aatcaacccg
    21541 tcagcaccga tgatctggcg aatttcatca acttcgcgac cgtgagcgat cagttccgtg
    21601 gcgctcggca tatcaatacc ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg
    21661 aggtacactt tcttcgctcc ggcttcgcgt gccatctcga taatctgctc agaagtggtg
    21721 ccacggacga tggagtcgtc gaccagcagg acgtttttat cgcggaactc ggcgcggttg
    21781 gcattcagtt tacggcgcac ggacttacga cgcagctgct ggcccggcat gataaaggtg
    21841 cggccaacat agcggttttt aacgaagccc tggcggtacg gtttgcccag aatacgagca
    21901 atttccagcg cgatatcaca cgaggtttct gggatcggga tcaccacgtc gatatccaga
    21961 tcttcccatt cgcgggcaat tttctcgccc agtttcgtgc ccatattcac acgcgcgctg
    22021 taaacggaaa ttttgtcgat gaacgagtcc gggcgagcaa agtatacata ctcaaacagg
    22081 cacggattgc tgaccggatt gtcagcacat tgacgggtaa acaactgccc ttcttcagtg
    22141 atgtaaatcg cttcgcccgg cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc
    22201 gctacgcttt cggaagcgac catatattct gtacggttct cgtcaatatc acgttttccc
    22261 agtaccagcg gacgaatccc gtttggatcg cggaaagcaa ccataccgtg gccgataatc
    22321 atcgccacac aggcatatgc gccgcggatt aagcggtttg tggcagcaat ggcagcgaaa
    22381 atattgtcgg cttccagcgg gtagtggcgg aagttgtcca gctcgctggc gaagatatta
    22441 agcagaattt ccgagtcgga agtggtgttg atgtggcggc gtttttcttc aaacagtttt
    22501 ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg caagcgtaat gccatacggg
    22561 gagttaacgt aaaacggctg cgcttcagag gcgctggagc tgccagccgt agggtaacgc
    22621 acatgaccaa tgcccatatt gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg
    22681 ctcaccagcc cgttcgcttt acgcaaacgg aagcagttat tggcatctat ggtgatgatg
    22741 ccggcggcat cctgaccgcg atgctgaagc accgttaagg catcataaat cgactggtta
    22801 accggcataa caccggcgat accgacaata ccgcacatac gtctttttcc tcgttaagcc
    22861 acatctcaga gcacttacgc tctgggcaag aaacttgacg agctttgcag ataatcaaaa
    22921 aaccatctga tgataaaact gaactgcggg atcagctgtg atttgctcca gtcttcgctt
    22981 ttcgacaccc cggtaaagga gtcgagaaag aatagaatgg cggcaacaat caacacaccg
    23041 cgcaacgcac cgaaacagac gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc
    23101 accagctggc ctatcacgaa gttcacgata gcaccaacga tcagggtagc gataaatagt
    23161 accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac
    23221 aggtaagtgt agtaatgact ggcaacaaag aaagcacaac cccatgtcac cagcgataac
    23281 gcttcacgaa caaagccgcg gatcaggcta accagagagg aaaacgcaat caccgcgatt
    23341 atggcgtaat caatccagac catatgtgtc ccacgatttt acgccctgtc atcctgttcg
    23401 gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat
    23461 aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt tatgcctttg cggcatcgtg
    23521 caatgcgtgt cggatgcggc gtaaacgcct tatccgacct acggttctaa cactgcccaa
    23581 gcctgataag acgcgccagc gtcgcatcag gcaagactgt attaattcgg cgtatagccc
    23641 attaccacgc cacttaagcc agaaagttgc ttcaactcac ccagcgaacc tttcagctta
    23701 tctttcgagg catccggccc aaccagaata cgggtaattt taccctgcac tggcgtggat
    23761 ggcgacgtat aaacccgata accggcaccg cgcagcttac cgacaatctc attcacttta
    23821 tcggcatttt tcagcgcacc cagttgcaca acataggctt tacccgttgg cgctgctttt
    23881 tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac cttcggttta
    23941 ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg caggttccgg ttcaaactcg
    24001 gtgttattgg ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc acctgcccgc
    24061 acctcttccg ctgcgccttc cggcggctgc gtcggcaacg cctgggtggc ggctggcatc
    24121 atatcaggct catcacgatc gcccgctttc ggcaccagcg ggatagccgc gaactcatcc
    24181 tgataatgtt ttttctgccc gtccagcagc cctggaagta caatcacccc cagcgccacc
    24241 agcacgatcg tgcccactaa ccgattctga aacttacttg ccaccgcttc tcctcgcgtc
    24301 aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca cacaccagca cggtgtcttc
    24361 cgctttagcg tccgccattg cggcatccca tgcctgcgca acgctatcaa atgatttgcc
    24421 gttacccaaa tgctcaagca gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc
    24481 acaataccag tcatcaacca cgcttttcaa ccaggccaga gttccggcaa tatctttatc
    24541 atgtagcata ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt tcatacgccc
    24601 ggtgagatat tccgccgcat gtggattatg cgcgacatca aaaataacgc gtggcgactc
    24661 gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt
    24721 ttcactgact tccagcccgc tggcacgcag tgccgccagc gctgttgcgg cattcggttg
    24781 cgggacaagc ggcaacggca gattttccag cgtgccgtga gcatcgctaa acgcccagtc
    24841 atgatcggtg acggaatagt tccactcaac gccccgacgt tgtaacagtg cacctttttc
    24901 ctgcgccaca tcagcaatgg tagaaggcat ttccggctca ccgacaattg ccggttttgc
    24961 gctgcgaaag atgcctgctt tctcgcgacc aatactttcg cgatctggtc ccagccagtc
    25021 ggtatgatcc agcgcaatgc tggttactac agccacatcg gcatcgacaa tattggttgc
    25081 gtccagacga ccgcccagcc ctacttccag aatcactacg tcaagttgtg cctgtttgaa
    25141 cagccacaac gccgacagcg taccgtactc gaaataggtc agggaaatat caccgcgtgc
    25201 cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct gcccctgcac
    25261 acgtacgcgc tcggtataac gcaccagatg cggtgaactg tagacgccca ctttgtaccc
    25321 tgccgccatc agaatcgact ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc
    25381 aacggtaaac acaaatggcg ctggtttcag gacgccaaga cgcgccgcga cctggctcac
    25441 gcgctcaagg ccgagatcga tagttttact gtgcaggttt tccagataag aaagccacga
    25501 agccagaggc gacgcggctt gaggagtgcg tttgataatc atggtatccg ctgattcgtt
    25561 acggtgagaa tagcaaaagg gcagagccag tggccctgcc cttatcagtt atcaggcctc
    25621 aggttcctga tccggtaccg ggggtaccac tacgccttca cgcggcgctt caggattcgg
    25681 cgctggcaga ttcatcaact tcgccagaat gctcgccagt ttcaggcgca tttccggacg
    25741 acggacgatc atgtcgatcg cgcctttctc gatcaggaat tcactgcgct ggaatcctgg
    25801 cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc cgattaacgc
    25861 tttcggttca gcgatgttga gatcgcccag catggcgaaa cttgcagaaa caccgcccat
    25921 cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc tcctgcattt ttgccagtgc
    25981 cgcagaggtt ttcgccatct gcatcagcga catcagtgct tcctgcatac gtgcgccacc
    26041 agaggcggag aagcagatca gcgggcagtg atcttccagc gcctgctcaa cggcacgcac
    26101 gaaacgtgca cccacaacag accccattga accgcccata aaggcgaact cgaatgccgc
    26161 agcgacaacc ggcattccat acagagtgcc tttcatcacc accagcgcat ctttttcgcc
    26221 agtttctttc tgcgcagatg ccagacggtc tttatacttc ttggagtcac gaaacttcag
    26281 cacatctttc ggctcaagct cgctacccag ctccacaagg cttccttcat ctaacaggct
    26341 atgcaggcga ttacgcgctg tcatacgcat gtgatggtca cacttcggac agacctcaag
    26401 attacgttcc agctcagcgc ggtataaaac ctgaccgcag ctatcacact tagtccacac
    26461 cccttcagga atgctcgcct tgcgggtggg agtaatgttg cttttaattc gttcaatcca
    26521 gctcattagg gacctttctg tctgaacctg gttcgatgcc agttttatct ttggggacgc
    26581 ataatgccat ttttgccccc aacagaccat gaatgttgca cattaaaaca taacagcccg
    26641 aaactttgga taaaaaagtg gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc
    26701 gcagcgcgtt tgtgacgaat aaattcgaaa accccaggca atattgaaac aaaaataatg
    26761 ccaacaatca tcagcttaag gttgctctga atgaagggga gcgtgccgaa gaaataaccc
    26821 gcgtaggtaa aaatcagtac ccacaacagt gcgccgatca cgttataagc ggcgaaatga
    26881 cggtacgaca tgtggcccat tcccgcaaca aacggcgcga acgttctgac gatcggtaca
    26941 aaacgggcga gaataatcgt tttgccgcca tgtctctcgt aaaactgatg agtcttatca
    27001 agataactgc gacgaaagat tttcgaatcc gggttactga acagtttgtc accaaataac
    27061 cgtccaatgg tatagttcac tgcatcaccg acaatagcgg caatcaacat aagcaccacc
    27121 atcatatgga cattgagatc gttagtttcc agcgacgcca atgcaccagc aacaaacagc
    27181 agcgaatcac caggcaggaa tggcgttacc accagccccg tttcgcagaa cagaatcaaa
    27241 aacagaatgg cataaaccca gacgccgtac tccgcgacca gctctgccag atgcacgtca
    27301 atgtgcagga taaaatcgat gaggaaataa atcaggtcca tatttgccta tgccttgtac
    27361 tcgtcatctt tcaggctgta actacgttgg ctacagcctg aaatgttccg agcattattc
    27421 tcgttagtcc gccagaaata gcgggcccat tggcggtttt ggaagatcat accggtcagg
    27481 gtaatccacc gcgaccagat acagcccttc cgcttttgcc gttgctgccg ccagcgttct
    27541 gtcctttgcc gccagcaact ctgctatcca gctctccggc tggttgtggg caccgacttc
    27601 catcaggctg ccgacaatat tcctgaccat atgatgtaca aaggcattcg ctttgatatc
    27661 taccaccaca taaggaccgt gacgcgtgac attaatatgc ataacgttgc gccacggggt
    27721 tcgggactgg cactgcaccg cacggaacga ggtgaaatca ttctcgccca gcaagcattg
    27781 cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta cccctttact
    27841 cagtaccgcc gggcgcagcc gatgattgta gatgatgtag cgataacggc gagccgtggc
    27901 gctaaatcgg gcatgaaaat catcaggtac agctttaacc caacgcacgg cgatgtcacc
    27961 aggtaaattc gcatttacgc ccaaggtcca cgccgcgtct ttgcgctgcg cggtcgtttc
    28021 gaaatgcact acctgcccgg taccgtgtac accagcgtca gtacgcccgg cgcagaagac
    28081 ggtaatgggt tcgttcgcca cctgggagag cgccttttcc agcttctcct gcacactgcg
    28141 gacttcattc tgccgttgcc agccgtaata cttactgccg tcgtactcaa tgcccagcgc
    28201 aattttataa actggcggtt gttgctggtc ggacattagt acagatactc ctgcaccagt
    28261 ttctcggcga tttttactgc catcagcgcg ccgccaaagc gaacgttatc ggccaccgac
    28321 cagaactgga cttgctccgg cataccgtag tcattacgca cgcagccaac agaaagatgc
    28381 ggcgtacccg aagcatcccc tacctgagtc gggaattcgt tctcttcaga gagcacaata
    28441 tcttcgccct gagcaaacgc atcacacgct tcttctgccg ccagcggacg cagtgcttca
    28501 aagttgacca tctgggcatg accgtagaat accggtgcct ggacgacgct tgccgaaatc
    28561 atcagccctt cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc
    28621 acgctacctt cgctatccgg caacaacggc agcatattga acgccagttg acgaccgaag
    28681 aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg cactctgccc cgctaacgca
    28741 tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc tggtaacact gatacgcgac
    28801 aaaccgccct gatcgattaa cggtttcagt gccgccagca gctggctggt cagactgtct
    28861 ggtacggcga tgacattccg gttgcggtaa tcggtcagta caaacgggtt tacttccggc
    28921 accaccagcg gtacgtcggg ttcgagagca aacagtccgc tgctgtcgat caccaggcaa
    28981 cctgagttgg tcgcttcttc aacccaggca gcggtagctt ctttgcctgc gacaaaaaat
    29041 gccagctgcg cctgcgtcca gtcgaattca gcggcatcct gcacggtgat tgtcttacca
    29101 ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc
    29161 gggaactgac gttcagccag cgtttcaagc agggcttcgc ccacagcgcc agttgcgccc
    29221 aggacggcaa tgttccagcc ttcagacatg gtggtttact ccagaaatag caaagctccc
    29281 tgccaatgtt acagcagaga gcatgaagaa gagattaacg tgccggatga tgaacggcgt
    29341 taaaacccag tttacacagc aatgatgccg cactggcgtc atcacaaatt acatacagag
    29401 atgaccattc acggcgctca agatagtttt tgcgcagttt atcgaactca cccggtatcc
    29461 cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc
    29521 ttttcagcgt cggttgatcg agcgggccat gcagcgtaat gcgaccaaac tctggcgcgg
    29581 gcagtaatgt atccagcgca acgtgctgtt catgcccaat aaacttgcta taggcttcaa
    29641 acacttgcgt agtaccgcgt gctttacctt ccagggtata acctgcgata tgcggcgtgc
    29701 cgatatccac ttttgtcagc aactccacgt taagttccgg ttcgccttcc cagacatcca
    29761 gcactacgct taacttctgg ccttcattca ggcaggtcag caacgcagta ttatcgacga
    29821 ctgcgccacg gcaggcgtta atcagaatcg ctcccggctt caggctacgg atcagttttt
    29881 catccgccag atgcagcgtt ttgtacggcc catctttgaa gagtggcgta tggaaagtca
    29941 gaatatccgc atgctgaact aattcatcca gcgagcggaa atcaccctca tccccacggt
    30001 cagcgcgagg cggatcgcaa agcaaggttt taatccctaa cgcttccagt cgcgcttgca
    30061 atcgacgtcc aacgttacca acgcccacga tccccaccgt acggtcgtgc agtgaaaatc
    30121 catcgcgttc ggcaagcatc agcagggagg agaaaacata ttccaccacc gcaatcgcat
    30181 tacagccagg tgccgcggaa aaaccaattc ccgcctgctt taaccatgct tcatcgacat
    30241 ggtcggtccc cgctgtggca gtaccaacaa atttaatggg ttttcctgcc agcaaagatt
    30301 cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg
    30361 ggattggacg cccgggaacc gcggtcacct cacccaaacg gctaaataag tcgcgggcat
    30421 aaggcatatt ttcatcaaca aggattttca cgtttgtgtt acctgtatga gacgagagtt
    30481 aaccggacaa gtgtgccata atctcgcggc caggcatact tgcgaagatt tcaggtataa
    30541 ggatacgtaa tgatacaacc tatttccggc cctcctcctg ggcagccacc aggtcaggga
    30601 gataacctgc cgtctggcgc gggcaatcag cctttatcca gtcagcaacg tacttcgctg
    30661 gaaagcttaa tgacgaaagt gacctcactg acgcaacagc aaagagcaga actgtgggcg
    30721 ggtatcaggc acgatattgg tctgtcggga gattcaccgc tgctttcgcg tcacttccct
    30781 gccgctgaac acaacctggc gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc
    30841 cagcttttag cgcaattagg ggagtattta cgtctgggga ataatcgtca ggcggtcacg
    30901 gattatatcc gtcataactt tggtcagacg ccgctgaatc agctctcacc ggagcaatta
    30961 aaaaccattc tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc gcagcagcgc
    31021 gaggcgaccg accgtccttt attaccggcg gagcacaatg cgctcaaaca gctggtgacc
    31081 aaacttgcgg cggcaacggg ggaacccagc aaacagatct ggcaatcgat gctggaactt
    31141 tccggggtga aagatggcga gttaattcca gcgaaactgt ttaaccatct ggtgacctgg
    31201 ctgcaggcgc gccagacgtt aagccagcaa aatacgccga cgctggaatc actacagatg
    31261 gcgctaaaac aacctttaga tgccagtgaa ttggcggcgt tatcggcata tatccagcaa
    31321 aaatatggcc tttctgcgca atcatcgctt tcttctgccc aggccgagga tattcttaat
    31381 cagctttatc aacggcgggt taaagggatt gatccgcgtg atatgcaacc gctgcttaat
    31441 ccttttccac cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata
    31501 ctgttagtcg cgattatcct gatgctggtc tggctggttc gttagccccg acgaaatgac
    31561 agtatcgtga caataattcc cagcaccgca gagatcgccc cggcaagaaa taccgaagag
    31621 taaccaaacg tggtcgccag cattcccgcc agcggcccgg agacgccgag ggcgatatcc
    31681 tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc
    31741 acctccacgc ccagcgcagg aaagataagc gaacatccgg ctccggttaa cgccgcgccc
    31801 gctaatgcga cccacgcccc tggggcttgc cagagcagca acaagcccac cgtttctaca
    31861 agcagagaga caatcgccac tttcacgccg ccaaaacggt ccggcatcca gccaaacatg
    31921 acgcgcatca cgacaaatgc gccgccaaac gcggtaagag taaagcccgc catcgcccat
    31981 cctttgctgg caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt
    32041 agtgccagcc ctaaccctgg tttccagata agcccgacaa cgctccacag cgatggacgt
    32101 tctcccgcca aggccggtac tttgcgcact gtgccgttac aggcccatgc cagtaagggt
    32161 aataccattg tggtgatcgc cagtgcggca aaaccgtaat ggctatgaat caacaggcca
    32221 agcggagcac caacagcgag ggcaccgtaa atcgccattc cattccatga catcactttg
    32281 ccagagtgtt ttggccctac gatgcctaac ccccaggtca gagcgcctgt cagtaactgg
    32341 ctttcaccaa agccaagaat caaacgcccg acgaccaaca gggcaaattt gaacggtgcg
    32401 gagacaggca aaatcgccgc cagcagcaac gcgccgccag ccagaccaca agctaacatc
    32461 ccctgaagcg ccgaacgttt tgcaccatat tgatcggcca gtcgcccggc atagccacgc
    32521 gtcagcaccg tagccagaaa ctgaatcccg acggcaatcc cgaccatggt attgccatag
    32581 cccagatcat gatgaacaaa cagcgggata accggcaacg gcagccctac ggtcatgtag
    32641 gtgagaaaaa ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg
    32701 gtttggctta cagcagtcat gcattactcc agaatgcagc gcaaggcgag gagtatcccc
    32761 gtctcatctc tctggtttca gggttacggt gcgttggcag gatttaacgc gtacgtcttt
    32821 tcagaaggaa atcgacaaag cgggaagttt gcctggaaca ggcggcgatt gtcaatgatg
    32881 tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc ggaggccgca ttagcgcgta
    32941 acgtcggatg cgacgctggc gcgtcttatc cgacctactc cgactactcc gacctactgc
    33001 gaattaatct ttcagcttgc gcattaccag cgtggcgttg gtgccgccga agccgaagct
    33061 gttagacata acggtagtca gttcgcgatc ggtcgtttcg gtcacgatgt tcagacccgc
    33121 agcctgctcg tccagctctt caatgttgat gctcggggcg ataaagccgt gttccagcat
    33181 cagcagagag tagatagctt cctgtacgcc agcagcgccc agagagtgac cggtcatggc
    33241 tttggttgca gaaatcgccg ggctcttatc gccgaacact tcacggatag ctgccagttc
    33301 tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc aggtaatcga ttggggtatc
    33361 aacgccatgc atcgccatct gcatacaacg tactgcgcct tcgccagacg gagcaaccat
    33421 gtctgcacca tcagaggttg cgccgtagcc aacgatttca gcatagatgt gagcaccacg
    33481 cgccagcgcg tgttccagct cttcaaccac taccataccg ccgccgccag cgataacgaa
    33541 accgtcacgg tgagcgtcgt aagtacggga ggctttttcc ggggtgtcgt tgtatttagt
    33601 agacagcgca cccatcgcgt cgaattcgca ggccatttcc cagcacagct cttcgccgcc
    33661 gccagcaaac acgatgtcct gtttgcccag ttggatctgc tctactgcgt taccgataca
    33721 gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta acgccatgaa ttttaaacgg
    33781 cgtggcgagg caggcagaaa cgccggatgc catcgctttg gtgaccacat acgggccaac
    33841 cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg aatacctgga aacgcgggga
    33901 accgccgcca gaacctgcaa tcaggccaac gcgcgggtta ttctggtaag cttccggaga
    33961 aaggcccgca tctgcgattg cctgctccat agaaaggaat gcataaatgg atgcgtcgct
    34021 cataaagcgc acaactttgc ggtcaatgag gccagtggta tccagtttta cgttgcccca
    34081 gacgtggcta cgcatgccgg aatccttcag ctcctgagag aaagtgatcc ctgaacgtcc
    34141 ttcacgcaga gatgccagga cttcctgctg gttattaccg atgctggaaa caatgcccag
    34201 gccagtaatc actgcacgtt tcattcaata cctctgtaag tcgcacatag agtaagtttc
    34261 gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg atcagccatt taatagagaa
    34321 atttgcgcag ccttacacac atcgctaaga tcgagccacc gcctgtaaga cgagtaactt
    34381 acgtgaaaca ctactccata caacctgcca acctcgaatt taatgctgag ggtacacctg
    34441 tttcccgaga ttttgacgat gtctattttt ccaacgataa cgggctggaa gagacgcgtt
    34501 atgtttttct gggaggcaac caattagagg tacgctttcc tgagcatcca catcctctgt
    34561 ttgtggtagc agagagcggc ttcggcaccg gattaaactt cctgacgcta tggcaggcat
    34621 ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca acgcttacat ttcattagtt
    34681 ttgagaaatt tcccctcacc cgtgcggatt tagccttagc gcatcaacac tggccggaac
    34741 tggctccgtg ggcagaacaa cttcaggcgc agtggccaat gcccttgccc ggttgccatc
    34801 gtttattgct cgatgaaggc cgcgtgacgc tggatttatg gtttggcgat attaacgaac
    34861 tgaccagcca actggacgat tcgctaaatc aaaaagtaga tgcctggttt ctggacggct
    34921 ttgcgccagc gaaaaacccg gatatgtgga cgcaaaatct gtttaacgcc atggcaaggt
    34981 tggcgcgtcc gggcggcacg ctagcgacat ttacgtctgc cggttttgtc cgccgcggtt
    35041 tgcaggacgc cggattcacg atgcaaaaac gtaagggctt tgggcgcaaa cgggaaatgc
    35101 tttgcggggt gatggaacag acattaccgc tcccctgctc cgcgccgtgg tttaaccgca
    35161 cgggcagcag caaacgggaa gcggcgatta tcggcggtgg tattgccagc gcgttgttgt
    35221 cgctggcgct attacggcgc ggctggcagg taacgcttta ttgcgcggat gaggcccccg
    35281 cactgggtgc ttccggcaat cgccaggggg cgctgtatcc gttattaagc aaacacgatg
    35341 aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc tcgtcggttt tacgaccaat
    35401 tacccgttaa atttgatcat gactggtgcg gcgtcacgca gttaggctgg gatgagaaaa
    35461 gccagcataa aatcgcacag atgttgtcaa tggatttacc cgcagaactg gctgtagccg
    35521 ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac aaattgcagc ggcattactt
    35581 atccgcaagg tggttggctg tgcccagcag aactgacccg taatgtgctg gaactggcgc
    35641 aacagcaggg tttgcagatt tattatcaat atcagttaca gaatttatcc cgtaaggatg
    35701 actgttggtt gttgaatttt gcaggagatc agcaagcaac acacagcgta gtggtactgg
    35761 cgaacgggca tcaaatcagc cgattcagcc aaacgtcgac tatcccggtg tattcggttg
    35821 ccgggcaggt cagccatatt ccgacaacgc cggaattggc agagctgaag caggtgctgt
    35881 gctatgacgg ttatctcacg ccacaaaatc cggcgaatca acatcattgt attggtgcca
    35941 gttatcatcg cggcagcgaa gatacggcgt acagtgagga cgatcagcag cagaatcgcc
    36001 agcggttgat tgattgtttc ccgcaggcac agtgggcaaa agaggttgat gtcagtgata
    36061 aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg gtaggcaatg
    36121 ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt ggcggagcag aaagatgagg
    36181 cggtaagcgc gccggttttt gacgatctct ttatgtttgc ggctttaggt tctcgcggtt
    36241 tgtgttctgc cccgctgtgt gccgagattc tggcggcgca gatgagcgac gaaccgattc
    36301 cgatggatgc cagtacgctg gcggcgttaa acccgaatcg gttatgggtg cggaaattgt
    36361 tgaagggtaa agcggttaag gcggggtaat ctgctctcgc agtgtttacc ggatgcggcg
    36421 tgaacgcctt atccgaccta cgtgtgacgt gtaggcctga taagacgcga taagcgtcgc
    36481 atcaggcatg gtgctccaga cgccgcaact tactgctgtg acgcctgctg aaacaggttt
    36541 tcccacatat cggtcaccag cgcctggtca cgcggcgaca attcaccggc accaatggct
    36601 ttttccagac tctggctaac ggtcgtatgc accgcctgag cggagtggtc atcaccactt
    36661 tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat acccactggc aaacaactca
    36721 tcatcactgg catggtcaac cataccgtcg attaatgcca gaatgcgtga ttcaaactcc
    36781 gcgatcatct tctttcctca ttgtaaaacg tggcgctggc tttagttgag cgcttccggc
    36841 cacgggaact gttccgccgt aagttccggc gtgtgataat aattttgtag tgctttaatg
    36901 aagcgtgccg ggcgttcagg aatgccgttt gctaggtaat ccatcacctg cgcatgtacc
    36961 cgccgttgaa acaccacacg atccggttcg aagtcgcctt ccagattgtc gcaactgacg
    37021 ttaaacggat atcccgccgc cacacagaac aaccagtcga gtgcctgcgg cttcacttca
    37081 acatcttcaa actggctttg cgtttgggca tcacgcccat ccgggcaata ccagtagccg
    37141 aaatcaacca gctcacggcg cgcttttccg gcaatacacc agtgcgaaat ctcatgaatt
    37201 gcgctggcat aaaagccgtg ggcaaagaca attcggttat acggtacttc cgcatcagca
    37261 ggaagataga tcggttcgtc gtcgccttta atcagacggg tattaaattc atcggcaaag
    37321 cagctattaa aaatttcaat caactgctca tagtggtgtg tactgttcat tagttcatcc
    37381 ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag taatttggca ctcatcaccg
    37441 ccgagacgat aacaatcatc gggcggatca gcttttgtcc tttgctcaac accagccgtg
    37501 aacccatgcg cgcgccgagg aactgcccca ccagcatgac aaagcccgtc gcccaaatca
    37561 ctttgccgcc gagaataaac agcagcagac cgccaatgtt tgacgttgcg ttgagtaatt
    37621 tggcgtgagc cgtggctttg gcgaggttga atccgcacag cgtaacgaag gccagcgcgt
    37681 aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa accgacgcag ccaccggcaa
    37741 ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc ttcttcaccc agctttggca
    37801 tcagcaagaa atagaggccg atacaaatca ccagaatggg caaaatctgc cgtaagacat
    37861 cagcctgaac gtactgtacc aacagtgcgc cgctcattga gccgacaaag gtcatggcaa
    37921 tattgagttt ctgatcgctt aaactaacca ctttgcggcg aataaagtag atagtggcgg
    37981 aaatagagcc gccgcaggct tgcagtttat tggttgccag cgcattagcg ggagacatcc
    38041 ccgctgccat caatgccgga atggtgagta acccaccgcc accggcaatc gagtcgataa
    38101 atcccgccag catggcgaca aaaaagagaa ctcccagcaa cagcggggaa accataaaca
    38161 ggctattaaa cgtttccatt agatcacgtg ctcatccagt agcgcctggc aggaaggcgg
    38221 caacggaggc ggtgtcttct tctcaggctt tgttgttccc ggttttggag gtgcaaacca
    38281 gctttgcagt tctgccccgc aaccatcgcc tggtggcggt aaaggttgat cttcacactc
    38341 cagactatcg gcaggacaac gtaatcgtac atgcatatgc gcgcgatgct ggaaccaggg
    38401 tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt gttgtttaat
    38461 cgccggatta acaaaaatgc gtgtgacgtc tttgtcctgg gcggcgagtt tgatcaagct
    38521 gaaaatttct ggcttccaca gcgtggagac aacgtgttta ccgtcgcggg aaactaagtc
    38581 cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga gttttcggca gttgcagaaa
    38641 gatatcgaca tccagtccgg tctggtggct ggcatgaccg ccgttgaaac gcccaccagc
    38701 gggcatcccc atatcgccaa tcagcaccgt acccatgccc agattgctca cctggctact
    38761 cagacgctgg ataaacatca ccagatccgg gtgaccgaaa tagcgacgct gatcggtacg
    38821 catgacctga taatgttcgg actgtatcgg cagcgtgtca gcaccgacga tacagccatt
    38881 agaaaaactg cctatcgatt gtgcgctacc cggcacaggt tgggttattt tttgccacgg
    38941 cgtcgctgcc aggctagcgc tactggcaag cagagccagc agcgcaatcg cggttttgtt
    39001 cattttttac cagcgtggaa tatcagtctt cacatcggca ttttgcgccc gctgccgtaa
    39061 caggtgatcc attaaaacga tcgccagcat cgcttcagcg atcggcactg cacggatccc
    39121 gacacaggga tcgtgacggc ctttggtgat catctcaact tcttcgccaa agcggttaat
    39181 agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc gccatatgag caatgatttg
    39241 ctgcccgctg ctgataccgc cgagaatgcc gcccgcatgg ttgctctgga aaccgtcttt
    39301 ggtgatttcg tcgcggttct ggctgccgcg cagcgccact acgtcaaaac cgtcgccaat
    39361 ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg gcgatgtcgg catccaggcg
    39421 gtcaaagacc ggctcgccaa gtccggcggg gacaccactg gcaacaacgg tgactttcgc
    39481 gccgatggag tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg cgtcgatttt
    39541 gtcggggtcc gggcagaaga acggattttg ttcaaccagc gaccagtctt tgatttccag
    39601 cggaatgtcg cccatctggg tcaagcagcc gcggatttca ataccaaatt tctcggcgag
    39661 atattttttg gcaatcgccc ctgccgccac gcgcatggcg gtttcgcggg cggaagaacg
    39721 tccaccgccg cgataatcgc gcagaccgta tttttgttcg taggtgtaat cagcatggcc
    39781 tgggcggaaa acgtctttaa ttgcgctgta atcctgagag cgctggtcag tgttttcgat
    39841 caacaagcca atgctggtgc cagtagtaac gccttcaaaa acaccggaga gaattttgac
    39901 ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc ccagggcgac gacggtcgag
    39961 gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat cgacgatgca
    40021 gccgagcgcc agcccatgcg attcgccgaa ggtggttacg cgaaagagtt gtccaattgt
    40081 gtttccagcc atcacggctc cgttattgtt gtgtttgcgt gtttacttaa tctttataaa
    40141 tcgcgaaata ttcacgggcc gccagcagct gctctttggt gagcataaac acaccatcgc
    40201 cgccgttatc aaactccagc caggtgaacg gaacatccgg atattgttcc ataagatgta
    40261 ccatgctgtt gccgacttca caaatcaaca cgccatcatc agcaaggtaa tctgccgcgt
    40321 ttccaagaat gcgacgtgtc agtttaaggc cgtcggtgcc agatgccagg cccagttccg
    40381 gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc cgcgtcgaca tacggcgggt
    40441 tagtgacaat cagatcgtat tgcactttcg gcaaatcacg gaacagatcg gaacgaatcg
    40501 gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc gccagcgcgt
    40561 cgggagagat atctaccgcg tcgacttccg cttccgggaa ggcataagca caggcaatgg
    40621 cgatgcagcc gctaccggta cacatatcta aaatatgctg cggttgcttg ctgatgagtc
    40681 cggcaaattt attgttgatc agttcaccaa tcggcgagcg cggcaccagc acgcgttcat
    40741 cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt caggtaagcc accggaatgc
    40801 gctcgttgac gcggcggatc acgcgttcaa caatacggtg tttttcgctg gaggtcagac
    40861 gcgcggtgcg catatcttcc gggatatcca gcggcaggta gagcgatggt aacaccagct
    40921 gtacggcttc atcccacggg ttatcggtgc cgtgaccgta ccagatattt gccgcgctga
    40981 agcggcttac cgaccagcgc aacatgtcct gaatggtttg cagctcattt actgcttcat
    41041 caacgaaaat tttatccacg tattcctcca gggcatgatc gcaataattt cggcggctag
    41101 tttgccatga agatgacgat aaatcagcat tcacgcgcgg tgagtgagga aaaatacgtt
    41161 taaaacgatc gattgtgcta cgagtcgggt aaactgtagg aaaattagaa atagagacag
    41221 ataaatgaaa aagaaaacaa cacttagcga ggaggaccag gctctgtttc gccagttgat
    41281 ggcggggact cgcaagatta agcaggacac gattgtccac cgaccgcagc gtaaaaaaat
    41341 cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct gatgccagcc attatttctc
    41401 cgatgagttt cagccgttat taaataccga aggtccggtg aaatatgttc gcccggatgt
    41461 cagccatttt gaggcgaaga aactgcgccg cggcgattat tcaccggaat tgtttttgga
    41521 tttacacggt ctgacgcagc tgcaggccaa gcaggaactg ggcgcgttga ttgccgcctg
    41581 tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg cacgggaagc atattttgaa
    41641 gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg atggcatttc accaggcacc
    41701 gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt gaagtggaag agtggttgcc
    41761 gccggagttg ccctgaatgc tggcagcaaa tgccggagca gcgtaaacgc cttatccggc
    41821 ctacaaatat gtgataattc aataaattgc aattcacttc gtaggcctga taagacgcat
    41881 cagagcaata ctacgaacta cagttcattc aatacatact gaaaacgaca cgtcgaatca
    41941 atcggttgcc cactactggt attcacattc accacaatat cgtgtagtac tttatccgtt
    42001 accggatgac gagttggcgt aaccgttacc gttgatttat cacaaccgta agaggtaaag
    42061 gttaacaatt ctgataccgc gcggcagtat gctcctctgc catcattatc agaaaaatta
    42121 ctgccatcac agagtctgcc gccttccatc aagtaggtcg aaagcgtggt ggtcactgtt
    42181 gaccccgtgg actgaattcg taacgcctta ttatggctga cataattttc actccaggag
    42241 atatcgtatg aagcgaccgg tatcgttggg catattgtgt tatgcactgt aggggccgta
    42301 tagcttccaa atccgggtaa atctgtatag gaataaaagg atgcgatcca agggcaacca
    42361 taatcatcag atgcaacggt aattttaaac gtcatatatg cacaacgaaa aattttcgta
    42421 tctatgccag ggcaatttga gttattaatt gtatcaaagt cagtcagtac caaagttgga
    42481 gctgaacccg acccagacca ggaaataacc cgcaacgtat cgcctttata cgcaaaagaa
    42541 tcatatttcc catatgcaac ctcctttggc gttatcaccg taacattgaa aacactgctc
    42601 gctacagcac cggttacctt gtccatggca taataaactc tcacactgtc aaaactcgca
    42661 ctcataagtt tatctccagc ataactctta gatgcgccga caacactcag aacgagcagc
    42721 attaggctcg cgaaaattat ggtccatttt ttcatttcag aaatactcaa aggttgccgt
    42781 cactgtggca ctaaagtctc cggacgttac atctcgcccg ctctttgcct gtaaccaggt
    42841 attaaaattt aggctgttat ttccctgatt cagcgggagc gtgacgcctg ttgtattgtt
    42901 gattggcacc gatgtgcctg ccgcagtttc aataccaatc ccagcgccgg atgctgatga
    42961 cgaggagtca aaggccagaa agccgggtag agcactatct tctgttcctg tcaacgttac
    43021 tttgacatta aacaatgtag aactatgaca atctttcaat tgaaacacca atggcactcg
    43081 ctctgactgc ccaaggttca ttaaatcgct ggcggcaatg gcaggaaaat gtacttctgc
    43141 caactggcta ccattaatca ctggcgtaca cgatttactg attaaattgc cggtaaagtg
    43201 caaattatct cctgctattg tggttccact gcccagcaac atcaggctac taataaaaaa
    43261 caacgtacgc tttttcttca ttggtaatct acctgtaacg tggcccaggc ctcaaagtcc
    43321 gcctctggca gaacgacacc actctttttt actggcacag ccgttaacac cggtaaatcg
    43381 gtttcattca ccacaagtgg cgtattgatc gtaaaactct gccctacctg tttgagctga
    43441 atgcctaaac cagtgatatt gctgcttact gcagcattat caaaatcgct tggtgtgcca
    43501 ctccagctca aggtcatttc cattaatggg tcgcgaaccg ccgtatcgca ggtaatggaa
    43561 tacggcacgg tttgctcata attgctgcca ttaatgtcat caataagcac atcctgaaaa
    43621 gttatttcaa tggtttgagc gttgctaatc gtgcagttag gtggctgcac taaataaccg
    43681 tgaaaagaga cgtttgagag tgccatcgag caaaagcccc acagcaaaat aagcgagatc
    43741 cgtttcattg gtaatccacc acaattgtgg cactggcgtt aaattcagct gccgctaacg
    43801 ttgtaccact ctgtttcacc ggcacagctt ccagaacggg aactcccgag gaaaaattaa
    43861 atggcaacca tgagccagag gttaagtcga gtattgtgtg atcactactt ttctgaaccc
    43921 gaataccaaa cccaggaatc cccgtgctga taactgtttc accgttaatc acgaccgttg
    43981 tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt gttacaaaca agcgagtagt
    44041 caatgggacg tttataatca accccattga tcttattaat gaatacatta ccaaactcaa
    44101 ctgccgcacc atttaccgta caaggaggag gggcatcgac aagcacccgt aaattcatac
    44161 ttactgactt actcgctgca actgcaaagc cgctaccaac acaacatatc agtaacccag
    44221 ttaccgccca gacagaacaa gatcctttca tactggctct cccggcctca gcctttgcgg
    44281 ctctgctctt catcgacctg gcaactgtta ccgttgcaag tgaaaaagag cggcatccga
    44341 gcgccatagt cgttgacata ggtcagtacc ggcacgctac ccatcttcac attcagcggt
    44401 gcagaggttt tcggcgacat cactattggc gagaaaccgg ctgccggatt gccatttttc
    44461 tgtgtgctgg cattactgaa aatgacgtaa tacggtgtcg ggttattgac ggtaaattcc
    44521 tgaccgttgc gggttaaagt aaccttatat tgccaggggt ttttcatgct gacgttctcc
    44581 agcgccttcg gtcgccagaa caacttaata cgcgtttgta gtgctatttg cagcgtgttg
    44641 gctttgtttg attttggcgg gatctcacgc acgttgaagt agaacaggct ttcacgatcg
    44701 gcaggcaatt tgttgatatc cggcattccc tgcactttta cctgcccgtt catcattgaa
    44761 tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac tgatcttgtt acccttttca
    44821 tcttcgatcc agctttgggc cagataaggc agtttcggat cgttattgcg caacgtcacg
    44881 ctgattgatt tgtcgctttc attaaatatc aaacgggtac gatctggcgt tacagaagcc
    44941 tgggcagaca aactggcagc gctaagcagt aacgctagcg tcgtagcacc caatttggca
    45001 gagcaaagta aatcagacat ggatcattcc ttaatcacgt tattggttag ctgaaacaga
    45061 aacaggggga ttggtgggtg tcacctgttg cgtctgctcc tgaatgacag gctttatgtc
    45121 atcaggaaca accggcgcga cgttgccttt atgctggcat ggcagtaaca agccgttaaa
    45181 cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa tgcgcaacac cactccagaa
    45241 cacactcatg tgttcgccag gtttcacccc agccagataa acattgccgt catcatcgac
    45301 aaggcccact gtctgctcgt tatcattttt tacttctgcg ccaaacggag gatggctgcc
    45361 gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg atgacggcaa atttgcggta
    45421 gccaatcgcc ccttcagtta gcgtggcttg taccaccgac tgggttgctt cggcgttttc
    45481 cggcagtttg ttgaggtcga tatacgcctg attgcggtaa tagttattga cgtcagaaac
    45541 gacggcttta ccaaacatat tggtataaac agcagcaccg ttaccttcta ccggaacatc
    45601 ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc atattctggg tacggtgaag
    45661 tgcgccaccg tgggcagtaa gcgttgcgcc gccctgtaac gacaagcccg cagaggtgta
    45721 ttgcccttca tggtagttcg cgctgaggtc aacctgcgcc agcgaaccat catggctgta
    45781 atagccgtca acgctggtgt gtttgtcact ggtgccgacg ttcaactgat agtgagtcgc
    45841 gtcatcgaca cggctgaaat aaccgacctg actgctgtcc gtcccactgc catagttgcc
    45901 gttatagcta acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccctt
    45961 atccgcccgg ttgtcatact cgtagcggta gccagtcagg gaaacgctca tattacgaat
    46021 gctgcccata ttgaaatagt gggagagcat gatgttgtag tttgtctgct cctcacgatc
    46081 ccagtaagta tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg
    46141 gttataagtg gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact
    46201 ggcatccaga tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa
    46261 agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc
    46321 gtccagcgaa cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac
    46381 atcaaacgcg accgcgccga acgtagacaa atcgcgacca acgccaagtg cggcagattg
    46441 gtagttttca tcccccagcg cgccaccata gagcgaccaa ccattagcga tcccccagga
    46501 agcttcagca ccagagaaaa attcgccttc aacatgatgc ccccactctt gcggacgacc
    46561 catcatgatc ttatagcgca cctgacctgg acgagtgagg tatggcatcg aggcggtgct
    46621 gatgtcatat tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga
    46681 gacagaatca ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat
    46741 cacacgcccc atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc
    46801 tggcgcgtag ccgcgcaggt tgggaggcaa catttgatcg tcagtgctga cactgccacc
    46861 aacataatta aaaccgtcga aaatatcgga attgaggtaa tcttcgccca gcgccagttt
    46921 ggcttttaat gacggtaacg cccgccaggc ataatagcga ctccactccc attttttttg
    46981 tgtgtcatcg ccgctaaatt cctcgtcatc atcattactg cgggtatgtt gatagttggt
    47041 ctgccagtca gcgcgcatac gccatggccc caggttaacc ccgaccgtcc cgttgccgct
    47101 gatctcgtta ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt tgatgctgta
    47161 gtccgcgacg atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc agtcgggata
    47221 agtatattcg aggtaagcct gtggcagtga aatgactaat gcggactggc ttaaatcagc
    47281 cttaatttcc atgccttcca gttgaccgga tttcaggcat ttagcatcgt ggctccattg
    47341 cagatttttc gccacgtctt cttttaaacc aaactgcgct accagttccg gtgtcagaca
    47401 agcatagctt ttgctcgcgt catcttcacc agcataccag taaatatcgt actcttccgc
    47461 caatggctgt ttatttagtt gaacctgtaa attgtatttt ccgggctcaa catatccctg
    47521 gctggaaaaa cgcttcagat caatttttgt gtcgcctttt aattccagaa aacgggaatc
    47581 aaactgaatg tcgtcttcag cccagacact gctataactc cctgacattg ccagcgcaat
    47641 gcaccacgga agaatccgta atcgaaaaag agaatggtca ggcatacacg tttctatccg
    47701 tttttattct tgttcggtca aaatccattt aaatccccca tagtagtgtc gctatggggg
    47761 tatgccttac aggtaagtaa tctggaaggt ggtatttgct tcgaaattac ccagatctgg
    47821 cgcatcagct gcgccgacca gccaggcttt aaagttcagt gtctgcttcg ctttgccttt
    47881 attggtagaa gtgtctttca ctattttctg gtcaataccc atgccgcttt tgtaagaggt
    47941 acccagagag tcaccgatcg ccaggctaac attgttaaat gccgcaccgg tatcggtatt
    48001 gaaaatggtg taataattgc cgctatttgc agaagaaacg gtaccagtaa aggtagtagt
    48061 cattgtttcc tgagtatcaa atacgcaatc ttgcagacga atctggaaac ttttcggcgt
    48121 ggtggtgccg ttatttttca gtttttcggc accgatatca cccatatcaa cttccagttt
    48181 gtgatcgtcc ggaacgatag aacacactga gtcttcaatg gtgccgtaaa aacgtgcagt
    48241 accgttgtta cccgcagcga ttgttgccgt agaggtgaac acgcccatca ccattgccgc
    48301 agcaatagct gttttaacaa acttactc
//
LOCUS       DAPHJE010000020        47747 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPHJE010000020 DAPHJE010000000
VERSION     DAPHJE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.020"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..704
                     /gene="comR"
                     /locus_tag="RH222_001167"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="RH222_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV3796221.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(766..1305)
                     /locus_tag="RH222_001168"
     CDS             complement(766..1305)
                     /locus_tag="RH222_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV3796222.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RH222_001169"
     CDS             complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RH222_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV3796223.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3244..3786)
                     /locus_tag="RH222_001170"
     CDS             complement(3244..3786)
                     /locus_tag="RH222_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3796224.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3809..4834)
                     /gene="nagZ"
                     /locus_tag="RH222_001171"
     CDS             complement(3809..4834)
                     /gene="nagZ"
                     /locus_tag="RH222_001171"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV3796225.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDSRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4845..5669)
                     /gene="thiK"
                     /locus_tag="RH222_001172"
     CDS             complement(4845..5669)
                     /gene="thiK"
                     /locus_tag="RH222_001172"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV3796226.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5650..6291)
                     /gene="lpoB"
                     /locus_tag="RH222_001173"
     CDS             complement(5650..6291)
                     /gene="lpoB"
                     /locus_tag="RH222_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV3796227.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYMLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6305..6682)
                     /locus_tag="RH222_001174"
     CDS             complement(6305..6682)
                     /locus_tag="RH222_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV3796228.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPITVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6685..7044)
                     /gene="hinT"
                     /locus_tag="RH222_001175"
     CDS             complement(6685..7044)
                     /gene="hinT"
                     /locus_tag="RH222_001175"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV3796229.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7378..9567
                     /gene="fhuE"
                     /locus_tag="RH222_001176"
     CDS             7378..9567
                     /gene="fhuE"
                     /locus_tag="RH222_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV3796230.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNCYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9627..11060)
                     /gene="ptsG"
                     /locus_tag="RH222_001177"
     CDS             complement(9627..11060)
                     /gene="ptsG"
                     /locus_tag="RH222_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV3796231.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11355..12152)
                     /locus_tag="RH222_001178"
     CDS             complement(11355..12152)
                     /locus_tag="RH222_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3796232.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVNFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDSSRLQSIR"
     gene            complement(12163..13167)
                     /gene="holB"
                     /locus_tag="RH222_001179"
     CDS             complement(12163..13167)
                     /gene="holB"
                     /locus_tag="RH222_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV3796233.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13164..13805)
                     /locus_tag="RH222_001180"
     CDS             complement(13164..13805)
                     /locus_tag="RH222_001180"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV3796234.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13795..14817)
                     /gene="yceG"
                     /locus_tag="RH222_001181"
     CDS             complement(13795..14817)
                     /gene="yceG"
                     /locus_tag="RH222_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV3796235.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14820..15629)
                     /gene="pabC"
                     /locus_tag="RH222_001182"
     CDS             complement(14820..15629)
                     /gene="pabC"
                     /locus_tag="RH222_001182"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV3796236.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLHDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15749..16990)
                     /gene="fabF"
                     /locus_tag="RH222_001183"
     CDS             complement(15749..16990)
                     /gene="fabF"
                     /locus_tag="RH222_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV3796237.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17078..17314)
                     /gene="acpP"
                     /locus_tag="RH222_001184"
     CDS             complement(17078..17314)
                     /gene="acpP"
                     /locus_tag="RH222_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3796238.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17525..18259)
                     /gene="fabG"
                     /locus_tag="RH222_001185"
     CDS             complement(17525..18259)
                     /gene="fabG"
                     /locus_tag="RH222_001185"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV3796239.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18272..19201)
                     /gene="fabD"
                     /locus_tag="RH222_001186"
     CDS             complement(18272..19201)
                     /gene="fabD"
                     /locus_tag="RH222_001186"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV3796240.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19217..20170)
                     /gene="fabH"
                     /locus_tag="RH222_001187"
     CDS             complement(19217..20170)
                     /gene="fabH"
                     /locus_tag="RH222_001187"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV3796241.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20238..21308)
                     /gene="plsX"
                     /locus_tag="RH222_001188"
     CDS             complement(20238..21308)
                     /gene="plsX"
                     /locus_tag="RH222_001188"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV3796242.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21389..21562)
                     /gene="rpmF"
                     /locus_tag="RH222_001189"
     CDS             complement(21389..21562)
                     /gene="rpmF"
                     /locus_tag="RH222_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV3796243.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21614..22135)
                     /gene="yceD"
                     /locus_tag="RH222_001190"
     CDS             complement(21614..22135)
                     /gene="yceD"
                     /locus_tag="RH222_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV3796244.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPVQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22334..22918
                     /gene="yceF"
                     /locus_tag="RH222_001191"
     CDS             22334..22918
                     /gene="yceF"
                     /locus_tag="RH222_001191"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV3796245.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTL
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23030..23989)
                     /gene="rluC"
                     /locus_tag="RH222_001192"
     CDS             complement(23030..23989)
                     /gene="rluC"
                     /locus_tag="RH222_001192"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV3796246.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24562..27747
                     /gene="rne"
                     /locus_tag="RH222_001193"
     CDS             24562..27747
                     /gene="rne"
                     /locus_tag="RH222_001193"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV3796247.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQKVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27944..28897)
                     /gene="flgL"
                     /locus_tag="RH222_001194"
     CDS             complement(27944..28897)
                     /gene="flgL"
                     /locus_tag="RH222_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV3796248.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGFRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28909..30552)
                     /gene="flgK"
                     /locus_tag="RH222_001195"
     CDS             complement(28909..30552)
                     /gene="flgK"
                     /locus_tag="RH222_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV3796249.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30618..31559)
                     /gene="flgJ"
                     /locus_tag="RH222_001196"
     CDS             complement(30618..31559)
                     /gene="flgJ"
                     /locus_tag="RH222_001196"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV3796250.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31559..32656)
                     /gene="flgI"
                     /locus_tag="RH222_001197"
     CDS             complement(31559..32656)
                     /gene="flgI"
                     /locus_tag="RH222_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV3796251.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32668..33366)
                     /gene="flgH"
                     /locus_tag="RH222_001198"
     CDS             complement(32668..33366)
                     /gene="flgH"
                     /locus_tag="RH222_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV3796252.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33419..34201)
                     /gene="flgG"
                     /locus_tag="RH222_001199"
     CDS             complement(33419..34201)
                     /gene="flgG"
                     /locus_tag="RH222_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV3796253.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34373..35128)
                     /gene="flgF"
                     /locus_tag="RH222_001200"
     CDS             complement(34373..35128)
                     /gene="flgF"
                     /locus_tag="RH222_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV3796254.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35148..36353)
                     /gene="flgE"
                     /locus_tag="RH222_001201"
     CDS             complement(35148..36353)
                     /gene="flgE"
                     /locus_tag="RH222_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV3796255.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36378..37073)
                     /gene="flgD"
                     /locus_tag="RH222_001202"
     CDS             complement(36378..37073)
                     /gene="flgD"
                     /locus_tag="RH222_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV3796256.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37085..37489)
                     /gene="flgC"
                     /locus_tag="RH222_001203"
     CDS             complement(37085..37489)
                     /gene="flgC"
                     /locus_tag="RH222_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV3796257.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37493..37909)
                     /gene="flgB"
                     /locus_tag="RH222_001204"
     CDS             complement(37493..37909)
                     /gene="flgB"
                     /locus_tag="RH222_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV3796258.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38064..38723
                     /gene="flgA"
                     /locus_tag="RH222_001205"
     CDS             38064..38723
                     /gene="flgA"
                     /locus_tag="RH222_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV3796259.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGISDEVR
                     VSIRTAPSLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38799..39092
                     /gene="flgM"
                     /locus_tag="RH222_001206"
     CDS             38799..39092
                     /gene="flgM"
                     /locus_tag="RH222_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV3796260.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39097..39513
                     /gene="flgN"
                     /locus_tag="RH222_001207"
     CDS             39097..39513
                     /gene="flgN"
                     /locus_tag="RH222_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV3796261.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSADISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39553..41088)
                     /gene="murJ"
                     /locus_tag="RH222_001208"
     CDS             complement(39553..41088)
                     /gene="murJ"
                     /locus_tag="RH222_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV3796262.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41198..42121)
                     /locus_tag="RH222_001209"
     CDS             complement(41198..42121)
                     /locus_tag="RH222_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3796263.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42123..42770)
                     /locus_tag="RH222_001210"
     CDS             complement(42123..42770)
                     /locus_tag="RH222_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV3796264.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42781..43365)
                     /gene="rimJ"
                     /locus_tag="RH222_001211"
     CDS             complement(42781..43365)
                     /gene="rimJ"
                     /locus_tag="RH222_001211"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV3796265.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43601..44809
                     /gene="mdtH"
                     /locus_tag="RH222_001212"
     CDS             43601..44809
                     /gene="mdtH"
                     /locus_tag="RH222_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV3796266.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSVGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44873..45520
                     /gene="grxB"
                     /locus_tag="RH222_001213"
     CDS             44873..45520
                     /gene="grxB"
                     /locus_tag="RH222_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV3796267.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45654..46214
                     /locus_tag="RH222_001214"
     CDS             45654..46214
                     /locus_tag="RH222_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3796268.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46320..47366
                     /gene="pyrC"
                     /locus_tag="RH222_001215"
     CDS             46320..47366
                     /gene="pyrC"
                     /locus_tag="RH222_001215"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV3796269.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47440..47685
                     /gene="dinI"
                     /locus_tag="RH222_001216"
     CDS             47440..47685
                     /gene="dinI"
                     /locus_tag="RH222_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV3796270.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11536 a        12901 c        11888 g        11422 t
ORIGIN      
        1 ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccag caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccagattc aggagatatc gccaatacgt
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctaatgcaga
      661 ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccattac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc accacccagc gcccccacaa cagtggcgac
     1021 atctttacca cgaccaccac caaactgatg ccctatcacg ccgccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccccgtt caaacacgtt cagactggcc actgccgcta cgcccagcgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa cacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaagg attcggagga gtctgaagga atgcctgatg
     1441 cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggatgagg
     1501 cgtttatgcc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa
     1621 tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg cgtcaggtta cccatcaggc taccgacggt ggagaagttc gacagcgata
     1741 ccagcgaacc atgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca
     1801 gtatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaatg tctggatcgc
     1921 gggtggtttg cagcgtcggt tccaccacca gctggttgat acggttagtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccacaccatc agatcagcct
     2041 caatatattc gccatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca gagatacgtg
     2161 gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcaggcc tttgtaaccg tagctgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg ccatttgcgc
     2341 ccaggttggc ggagtatttc aggaagagat tcagcatctc ctggtgaaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggcgtattg aaatcgttag
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 gttcaccttt ctcatcgcgc agttctgcaa tagtgattgt tttcgcttca cgatcaatat
     2581 caatgacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg gttacgatcg accagcgtaa ttttggcttt tttcttacgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatctttt
     2821 tcaatggcgt agtcaacgtg acccccttaa atttattaac caattgttaa ttaatggtta
     2881 ttaacatagc tcttaattaa caacaggtta cagcaatgaa aatgttcttc gctattgaga
     2941 ataacatgaa tggtgcattg gtcataccaa aattgatgtt aatcaagttt tgttgctgaa
     3001 aagataggcg actttaccgg gtgagatcaa aaaatagagt ttattgttta ttatttgagc
     3061 aaattcggaa gagtaatata gggtgaagcg tatatcgagg gcgtttggtc gtcctgataa
     3121 gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg gatgcggcgt ggacgcctta
     3181 tccgacttac agaattcgga gcgcatcagg taccggtaag gttacctgat gcgacgaggg
     3241 catttacccg agggttttga acgctttaat gcgctgtaaa tgcggggaga tattcttgaa
     3301 tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa tgatgcaact cttcagatgt
     3361 ccgctggctg ttaagtgctt catcattacg cgacaaaata accagacaac gatcgcgatt
     3421 cttctcgcgg aagttggtca cacacttagt ggcaatatcg gcatactctt ccgggcgatc
     3481 aatcttgcct tccatgttct cataagggaa caaattaggg ttgaagatca cctggcggat
     3541 atcgcaaaga aaaccaatcc gttccgccca gtatccgccc aggccaacgc cgcaaattag
     3601 cggacgctcg tcaacgttca gttgcaacat tttgtccact tctttaagca gatgctgcat
     3661 atcatgtttc ggatgccgcg tactgtagct tatcaagcgt acatccgggt caataaactg
     3721 caattgtaag actttctcgt ggttacccgg actgttagag tcaaaaccgt gtaaatagat
     3781 aatcatcgct tcctcacata agccagggtt agtgacctgc tttctcttcc tgccagcgtt
     3841 cgtgtaactg attcagacgg gtgctgatcg ctttccagcg agccgagtcc atcagttcct
     3901 gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg ttctgccttg atcggtgaca
     3961 gattatctaa cacgctgacg gcccctttac gattattgca gaccaggatc atatcgcaac
     4021 ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc cataatcgcg gcaccttcca
     4081 tcgataaatc gtcagagaaa atcacgccgt caaaacccag ttcctgacgc aaaacggttt
     4141 tcagccagta gggagaaccg ctcgccggac gcgaatcaac atcactgtag atcacatgcg
     4201 caggcataat ggcgtcgagt ttattttcgc ggattaagga actgaagacc gacatatctt
     4261 tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt ttctttgtgt gagtctgccg
     4321 ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt cattccggct tcatgcatac
     4381 catcaataaa ccggctggca attgccaggg ctttttgtgg atcggcatga taagaacgct
     4441 cgccaatcgc cgcgctgata tgcccgacat ccagcactgg cgcaaagctg atatcaatat
     4501 ccatagcgat catttcgctg gccatcagcc aacccgcctc ttgcgccagt ttgccgccct
     4561 cttccattcc tgacagcgca gcgaatgatt gcgccgctgg caagcgggta aaaccttcac
     4621 gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac caccagatga ttgcgcgaag
     4681 ctgcgcggat ctggcgcacc agttcacgta actgggcagg atcatgatag ttacgcgtaa
     4741 agagaatcag ccctcccacc agcggatgcg caagtatttc acgctcttcc gcgtccagtt
     4801 cgtaaccttt gacatccaac attactggac ccacactgct ctccttattg ttttattaat
     4861 agctgccgcc aggtgtcatc ggccagcctg ataaattgtt gatcgccggt ttgtcgccag
     4921 cggtactcaa accaccctgc tttgagcatc agcagccagg gaaaccatcg cctgacctga
     4981 cgccataatt gcgccggata aatcttcgcg cgagtggcat agtcattgac caattgccgg
     5041 tgctgttcag tattttccac ccacaccgcc gccagttcca gcgcgatatc accatctcct
     5101 gcatactccc agtcgatgag ttttaaccct gacgcgctat gcactaaatt tccggcgtgg
     5161 acatccatat gcaatggact taagcgtaaa ggccgtggtt cccgcgcttt gcgcagacgt
     5221 tttaacattc gcagccaacc cactgtccgc cgcgccggat cgctttgctg ccagtacagt
     5281 tccagtaacg gcaacagcgt tattcgccag ccaaaacgtg gttgttgatg tagataatac
     5341 agcaagcctg ccagttcgtt ggtatccggc aaatacgttt ttaccgcgcc gggcagatag
     5401 tcgactacca tccagtcacg gagatataaa tgcggcttcg gtgcaatgca tgcgggtagt
     5461 tgtgataaag cccgatactg gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc
     5521 tgacgcacaa caaaacgctg gccctggtgt tcaatgagaa aactcccgcc actaagccca
     5581 ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc
     5641 gtgatgggat tattgctgcg aaacggcacc tttacctgac cagataattt cgcccgtctg
     5701 caccagcatc agctgcattt gtagggtcgg agcgttaacg ttgccagagg cgctggagta
     5761 tagcatgtaa tgagcgccga cattgcgggc aatgcctatg gctttactac gggtacctaa
     5821 actgtcctgc ggcgacaaac ctaactgttg cttagccatc gacagctgct gggcggaaac
     5881 cagggtaaat ttaccgttat tagccagcgc atttcgcagc gtttcggtcg cttctgcggc
     5941 attcagcgaa ccgttagtac ggttgttaac gctatcaacc agcaggacgc tacccgcagt
     6001 caccccgtca gccccaagca tcttactgac catcggctgc attgcgccat tccagtcata
     6061 atggcgaata tgcggcgcag gcggtgcagt ttgatcttcg tgctcaattg ggcctggctg
     6121 ctgcgggatc gtcggcaccg agggcactgt ggggacaggc tgttgtggct cggctggttg
     6181 ttccggcgct ggtttcactt cttctaccgg tgcaggttca cgttgcccca cacacccggc
     6241 gagaaacatc gccagcgcgg taatcaaggc gtagcgactc atttttgtca tcaagattca
     6301 ccccttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     6361 acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca
     6421 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaatg ggttcttgcc
     6481 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     6541 tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat
     6601 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacgcc agagacacca
     6661 gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg
     6721 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     6781 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     6841 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg
     6901 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     6961 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     7021 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     7081 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     7141 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     7201 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgacaaata
     7261 tgaatacgta tatttctcat ttgcatttac aagcaaaatt attcgcacac taaatataac
     7321 cgctcatcac cctcctccgg catgagcctg acgacaacat aaaccaagag atttcaaatg
     7381 ctttcaacac aatttaacag ggataatcaa tatcaagcca tcaccaaacc ctcactactt
     7441 gccggttgca tagcattggc actattacct tctgccgctt ttgctgcacc agccactgaa
     7501 gaaacggtga ttgttgaggg ttcagccaca gctccagatg atggcgaaaa tgattacagc
     7561 gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat tcctcagtcg
     7621 gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct gggcgaagtg
     7681 atggaaaaca cgctggggat cagcaaaagt caggcggatt ccgatcgtgc tctttattat
     7741 tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta ttttgaatcg
     7801 cgctggaatc tgggcgacgc actttctgat atggcactgt ttgaacgcgt agaagtagtg
     7861 cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat taatatggtt
     7921 cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata cggtagctgg
     7981 aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg taaaatccgc
     8041 gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta caacagtgaa
     8101 aagaccttct tctcgggcat tgtcgatgct gatttaggcg atcttacgac gctttcagcc
     8161 ggttacgaat atcagcgcat tgatgttaat agccctacct ggggcggttt accgcgctgg
     8221 aatactgatg gcagcagcaa cagttacgat cgtgcacgca gtaccgcacc tgactgggcg
     8281 tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt tgctgatacc
     8341 tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa aatgatgtat
     8401 gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag taattatgga
     8461 cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa agttgatgcg
     8521 ctggatttgt tcgctgacgg tatttatgaa ttgtttggtc gtcagcacaa tctaatgttt
     8581 ggtggcagtt acagcaaaca aaacaattgt tacttcagtt catgggccaa catcttcccg
     8641 gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga ctggtcacca
     8701 cagagcctgg cacaggacga taccacacat atgaaatcgt tatatgctgc cactcgagtc
     8761 acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg gcgggttgat
     8821 acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct ggtgtttgac
     8881 atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc gcaaaatgat
     8941 cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga gctgggtctg
     9001 aaatcggact ggatgaatag ccgtctgacc accacgttag ccatcttccg tattgagcag
     9061 gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga aaccgcctat
     9121 aaagcggtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg cgcaattacc
     9181 gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa cgaaggaaac
     9241 gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta tcggttgcct
     9301 gtcatgccag agttgacagt cggcggtggt gttaactggc aaaatcgcgt gtataccgac
     9361 accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct ggtggatctc
     9421 ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa taacctgttc
     9481 gacaaaacct acgataccaa cgtggaaggt tctatcgtct acggcgcacc gcgtaatttc
     9541 agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc catctggctg
     9601 ccttagtctc cccaacgtct tacagattag tggttacgga tgtactcatc catctcggtt
     9661 ttcaggttgt cggatttagt accgaaaatc gcctgaacac cagaaccagc aaccactacg
     9721 cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc agcaacgctg
     9781 acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc accaaatgca
     9841 gcaaccagag ccggtgccat ttcgctggta cctgtcgctt ttgcatcttc agtcgcgtct
     9901 tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat ggtgtagtaa
     9961 acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct gttaccagac
    10021 agaacgatga agtcgatcag accgtgcgag aacgacgtac cgtcacgcat ccccagaaga
    10081 atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag gatcggcgca
    10141 acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga ggtcagcgcc
    10201 gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga gtgccagata
    10261 gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga cagtttaccc
    10321 gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg acctgctgcg
    10381 ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg cagaccaaac
    10441 ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg gttctggtaa
    10501 gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat gaaggacagc
    10561 acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg tttaccggca
    10621 aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat gtacgctgcg
    10681 atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt agaggcgatt
    10741 tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt aaccatgatg
    10801 ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa gccgagggcg
    10861 acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc ttctgccata
    10921 acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc cagcagaata
    10981 cctgcgatag gcagtacgga taccggcagc atcagcgatt taccgacctt ttgcaggtta
    11041 gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt tttttacgtt
    11101 ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc gccctttatt
    11161 tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac gtttcaactt
    11221 cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg
    11281 ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta attacgagct
    11341 ttaaaaaaac tcattcaacg gatggattga aggcgggaag agtcgatgtg aaacagacgg
    11401 gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc
    11461 atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggtacc
    11521 ggcgcaaggt aaggtgagtc agtttccacc agtaaccgat ccagggggac ataacgcgca
    11581 gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat
    11641 ccgagatcca gtaatttgcc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca
    11701 ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcgcg ggcgtcacgg
    11761 gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg gatgaaggac
    11821 tcttgctgac gaactttagt ttccggtgtg taataataat ccagcccggt ttctcccagc
    11881 gctacaacac cttcttctgc cgccagacgg cgtaaatctt ctacatcgta gggatcattc
    11941 tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc taccagatcc
    12001 cgcatgtgta aatagcccgg taatgttgtg gcgactgcca gacaaaaatt cacatcgcgt
    12061 gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca
    12121 tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg
    12181 tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag
    12241 ctcgcggttg atgcctgtga cagacattaa ctgttcacga atgtggcaaa catcccccag
    12301 tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcgacgacaa ggcccggcac
    12361 atcaacattg gtcacctgcg cagcaccatg atggcgtttt agcgcatcca tcagcaacgt
    12421 tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcca gcagcgaata
    12481 ccagtcgccc gatggcacgc tatatgccaa tgcctgacac aatgtttcac gagcctgcca
    12541 gttatctccc tgaaacaacg ccagtgccgc gccaggcgaa ccggcgctta agcgcaatgc
    12601 ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg
    12661 ttccggcggc ggcgcaaggt aatgtagccg acaacgacta cgtaatgttg ccagtaaacg
    12721 ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt caagcgtttt
    12781 cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc agacaacttt
    12841 cgcaccacct aagcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat
    12901 gcccaaagca tttttccctt tttcgggagc cagggtgtag taatcgggat gcgtgccagc
    12961 ctgcatcaac tgacatccac gacagtgacc gcaacttttg tgaccctgcg gttgttggca
    13021 gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg
    13081 aatgagcagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt tttcgaaatc
    13141 aggtcgtaac catggatacc atctcatgcg tccaactcct tcacccagtg ggtcacggta
    13201 gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatgctt
    13261 ttatcttgtg ctgccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaaagac
    13321 tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc aacttccggg
    13381 gtaacatcga gatagagcgt taagtcgggg cgaaaatccc cgagaacagc atcacgcagt
    13441 gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg agtggagaga
    13501 tcgtggcgat cgccaatcac ccaggtgccg ttagccagcg caggtttgat gactgtttct
    13561 accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt aatgacttca
    13621 tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtaccg
    13681 ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcgagcgt ctcaaccacc
    13741 acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg
    13801 cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat gactggcaag
    13861 attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt
    13921 tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg cacccggcgg
    13981 caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag
    14041 tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct gcaagcgcat
    14101 accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac tggcaacggc
    14161 ggtttctttt tcgataattg atgccatcgt caccagctga tttttatctt tataaggcag
    14221 accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg
    14281 cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca
    14341 accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg cgtacttatc
    14401 gtcgctcagc gtatgcttga tatacggggc ctcacgcaat tgcttgaggt aatcgctcag
    14461 acgcatccct tctaccagtc gcagagggaa ctgtgcttct ttaccgcttt ccagcaactt
    14521 cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga
    14581 aagatccggt tcgatacgca gcagccattg aaaaacccgt gggcgattga tgatcttatc
    14641 ggcataaagc tgttcaccga gcgccagacg tccggtccct ggcttcaggg taaatatcgt
    14701 ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc
    14761 agcggcgata cccagtacca ccagcaataa caagataatc aataacactt ttttcatgac
    14821 taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag
    14881 acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca
    14941 tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata agaggattgt
    15001 gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc
    15061 ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca ttccgtaacc
    15121 cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcga
    15181 atcaatactt gctcaagtcg attaagatgt ttaatacctg caagatgagg attgcgcccc
    15241 agccgcaccg ggcttagcgc caacgtcatc ccctcgttac gcaaacggtc gtaatgtgca
    15301 ggataagccg taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct
    15361 cgcccgccac taccgcgact gatcacgact ttcagtacac cattttgctg ttctgctgcc
    15421 agcgttttca tctcttgttc aagctgaggc cagaagtcac aggaaatcat caaccgctga
    15481 caagcatcat gtagtcgctg gatatgcgca gataataaac tgactttacc gtcgataact
    15541 ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgccagcgat
    15601 tcctgcttat aaccgttaat taagaacata ccagctcctt atcagagaat tgttagtgtg
    15661 gcagcatgtt cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg
    15721 accttttata agggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag
    15781 tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc tgacgtgctt
    15841 catgcggtac gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg
    15901 gaacagcctg atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac
    15961 ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt gcagcttcac
    16021 cgaagatggt tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt
    16081 gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca
    16141 ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc
    16201 tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt
    16261 actcttcaag taccagcata ccggcaccat cgcccagtac gaagccatca cgctctttat
    16321 cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc
    16381 caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt
    16441 cagcatcgcc atacgcgata atacgcgcag catggccaat gttgtgcacg ccggaagtac
    16501 aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg
    16561 ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt
    16621 tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg
    16681 cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct
    16741 gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg
    16801 agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc
    16861 tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg
    16921 tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac
    16981 gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaaagataa
    17041 aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg ccgttgatgt
    17101 aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga atctcagtat
    17161 caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc gcgcccaggt
    17221 cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc tgttcgccga
    17281 taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta tcaaaactcg
    17341 ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta aatcccggca
    17401 ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc aaatcatttt
    17461 gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt tgtgcaaatc
    17521 gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg atgtaagctg
    17581 cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg ccgaggcgac
    17641 ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca cgtgtcatgt
    17701 ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac gcaacttcgc
    17761 gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag ttggcctgac
    17821 cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca tgacgctttt
    17881 tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg ttggtttcga
    17941 taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga gtgataccgg
    18001 cattattgac caggatatcc acttcaccaa attccgcgcg aattttttcc agaacagatt
    18061 cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca cctaaataat
    18121 cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc gcgccacggg
    18181 ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt accagtgcga
    18241 tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct gccatcgctg
    18301 aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc aggccagtaa
    18361 gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc atgtactcaa
    18421 cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca cggatggcat
    18481 caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt ggtgcgttaa
    18541 aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc agcgcacagt
    18601 gagacggtac gctcactggt aacggcagag cgcgttttgc gcccgcagct ttacaggcag
    18661 cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc ggagagttaa
    18721 agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttt gcaatagacg
    18781 catcatccag accgatgatt gccgccatag cgcccgtgcc ttccggtacg gcttcttgca
    18841 tgaacttgcc gcgcatctca accagacgca ccgcatcagc gaaatcaatc acaccagcgc
    18901 aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt gctttaccgc
    18961 cctgctgctg ccatacgcgg tacagcgcaa cagatgcagt caacagcgct ggctgggttt
    19021 gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac aggtcgtagc
    19081 ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc gccgccatat
    19141 cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca aattgcgtca
    19201 tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat ccaccgccaa
    19261 aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca gcttcatcca
    19321 gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc accacgacat
    19381 tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg ttagcctgat
    19441 gcggaaccag ccagtccagt tgagaacggt caagattatt tgccgccagc gtctcatcaa
    19501 cgatgtgcgc cagttctgtt accgcaacct tgaagacttc gttgcccgcc atcgtcagat
    19561 gaattgaatt ctctggattc acacggtcag cattaggcag cgtcagcaac tcaccatagc
    19621 taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca gccagcaccg
    19681 cagcgcccgc gccatcgcca aaaataataa tagtcccacg atcggttgga tcgcaggtgc
    19741 gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat ttcacgtatt
    19801 gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg tcaaatgccg
    19861 ggcaaccttt aatgcccagc atgctttgaa tctgacaagc tgcgctcggg aaagcgtgcg
    19921 tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg cccgccatct
    19981 caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt ggcgcggcaa
    20041 tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg tccaccattt
    20101 tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta ccaataatct
    20161 tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca aactgcgagt
    20221 tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag cagctcaaaa
    20281 ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg tcgctgcacc
    20341 gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc accatgactt
    20401 tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc ggggttgagg
    20461 tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag tagccaccac
    20521 gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct gacaacaccc
    20581 tccatcgtct ttaatgtgac atttcctgta aagccgtcac aaaccagcac atctgtcttg
    20641 ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg gattgtttta
    20701 agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc accaatattg
    20761 agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac tgagcccata
    20821 atggcaaatt gcaccaacat tgtactgtca caatcgacgt tggcccctaa gtcaaggacc
    20881 accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg ctcaatcccc
    20941 tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt accggcactg
    21001 acacaggctt gcgctcgacc ttctttcacc agctccaggg ccacgcgcat tgaactccca
    21061 cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga ctgcgcagga
    21121 ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg cgtgatggcg
    21181 tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc ctgcaatgct
    21241 gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc cagggttaga
    21301 cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg ggaaaatcct
    21361 cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg ccgcggtagt
    21421 aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg tctacagaca
    21481 ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg gaacgggttg
    21541 gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc cccgatccaa
    21601 cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg tttggctttt
    21661 gcgcttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca cagtgttcag
    21721 aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc attgcaagca
    21781 gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc agtgcttcag
    21841 cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga tgagtaaacg
    21901 gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg tttaacactg
    21961 cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg tccacactga
    22021 ctacggattc ggcgacgcgc tcaacctgaa caggggtata gataccctgg taatcaaggc
    22081 gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt tgcatagggc
    22141 gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt ggttgccttt
    22201 tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg ctgataacga
    22261 acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata cgctatatct
    22321 gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc gtcgcgctct
    22381 tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg aaaccccacg
    22441 cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag cgcaatctct
    22501 ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg ttcttgatgg
    22561 cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac gcaaagccag
    22621 cggcaatatc gtcaccctct atactggact ggcgttgttt aattcggcga atgggcatct
    22681 gcaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgagcgagg cggagattga
    22741 taattacgtg cgtaaagagc atcccctgca ctgcgcgggt agctttaaga gtgaaggatt
    22801 tggcattacg ctgtttgagc gtttagaggg gcgtgatcct aacacgctgg ttggtttacc
    22861 gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga tgggatagtt
    22921 ttttgcgccg gatgtggtta cgtcgcatcc ggcaatcggt gctggcggcc tgatgcggcg
    22981 taaacgcctt atcaggccaa ccgcccatta tcaggtttat atgcttgtat tagcgcgcgt
    23041 tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggagcttcg atacgcatca
    23101 cctcaccggt ccccggatga gtaaacttca acgctgcagc gtgcaggaac aggcgattta
    23161 atcccgtgcc tgcttcagtg agctgcctgt caaattcacg gtcaccgtag cgatcgtcaa
    23221 aggcaatcgg atgacccgca tattgtgtat gcacacggat ctggtgagta cgccctgtta
    23281 ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta aagcgtgttt
    23341 ccgacggttt gccttcctga ctcacacgca cgatacgttc gccgctttgc agaatatttt
    23401 tcagtaacgg tgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc accagcgcca
    23461 ggtaatcttt ttgcatccct ttttcacgta attgctcatg cagagaacgc aacgccgagc
    23521 gttttttcgc taccagcaaa acacctgagg tgtcccggtc aagacgatga accagttcaa
    23581 ggaaccgcgc ttccgggcgc aacgcccgca aaccttcaat aacgccgaag cttaaaccac
    23641 tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatgtga tcatcttcat
    23701 ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc gcctcttctt
    23761 cccgctcagc aacgcgaacc ggtggaatac gcacctcatc acccgcttcg agtttatatt
    23821 caggcttaat acgttttttg ttcacccgca cttcgccttt acgcaaaata cggtaaatca
    23881 tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt tgccccgctt
    23941 cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg gttggcgatt
    24001 ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta cttttgcacc
    24061 ttattacttc actgcgtgat cactttattg atggttatta aaccaatcac cagcaagaag
    24121 tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta taacaaggct
    24181 tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga aattttacgg
    24241 aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg tattgatctt
    24301 tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat atttctggag
    24361 agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa aaacgggcaa
    24421 ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg cgactagaca
    24481 cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag tcgtcaatgt
    24541 aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca gcaggaagag
    24601 ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga aagtccaggg
    24661 cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga accgagtctg
    24721 gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact aaaagaaatt
    24781 gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat taaagatgtg
    24841 ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg caacaaaggc
    24901 gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat gccgaacaac
    24961 ccgcgcgcgg gtggcatttc tcgccgtatc gaaggcgacg accgtaccga attaaaagaa
    25021 gcactggcga gccttgaact gccggaaggc atgggactta tcgtgcgcac cgctggcgtc
    25081 ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gtctgaaaca ctgggaagcc
    25141 atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga gagcaacgta
    25201 atcgttcgcg cattccgcga ttacttacgt caggacatcg gcgaaatcct tatcgataac
    25261 ccgaaagtgc tcgaactggc acgtcagcat atcgctgcat taggtcgccc ggatttcagc
    25321 agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca gatcgagtcg
    25381 cagatcgaat ccgccttcca gcgtgaagtc cgtctgccgt ccggtggttc cattgttatc
    25441 gacagcaccg aagcgttaac cgccatcgac atcaactccg cacgcgcgac ccgcggcggc
    25501 gatatcgaag aaactgcgtt taacaccaac ctcgaagctg ccgatgagat tgctcgtcag
    25561 ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat gacgcctgta
    25621 cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga ccgtgcgcgt
    25681 attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgtca gcgcctgagc
    25741 ccatcactgg gtgaatccag tcatcacgtt tgtccgcgtt gttctggtac tggcaccgtg
    25801 cgtgacaacg aatcgctgtc gctctctatt ctgcgtctga tcgaagaaga agcgctgaaa
    25861 gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta cctgctgaat
    25921 gaaaaacgtt ctgcggtaaa tgccattgaa actcgtcagg acggtgtgcg ctgtgtaatt
    25981 gtgccaaacg atcagatgga aaccccgcac taccacgtgc tgcgcgtgcg taaaggggaa
    26041 gaaaccccaa ccttaagcta catgctgccg aagctgcatg aagaagcgat ggcgctgccg
    26101 tctgaagaag agttcgctga acgtaagcgt ccggaacaac ctgcgctggc aacctttgcc
    26161 atgccggatg tgccgccagc gccaacgcca gctgaacctg ccgcgcctgt tgtagcccca
    26221 gcaccgaaag ctgcaccggc aacaccagca actcctgcac aacctgggct gttgagccgc
    26281 ttcttcggcg cactgaaagc gctgttcagc ggtagtgaag aaaccaaacc gaccgagcaa
    26341 ccagcaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa gcctcgtcag
    26401 aacaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac tgaaggcagc
    26461 gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcagac tgccgagacg
    26521 cgtgagagcc gtcagcaagc tgaggtaacg gaaaaagcac gtaccaccga cgagcagcaa
    26581 gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc gcaacaagaa
    26641 gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga agaacgtgta
    26701 cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg ttacgagcaa
    26761 agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc tgccgaacca
    26821 attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct gccagtcgta
    26881 gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga caacggtggc
    26941 atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca gcgtcgtcgt
    27001 cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt agcgtgcgcg
    27061 tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg tccgcaagat
    27121 gtacaggttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat ggtgactgag
    27181 gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga agaaatggct
    27241 gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt tgaaacgacg
    27301 catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac cgagtctgat
    27361 gttgccgtag cccagaaagt cgcagaacac gcagaaccgg tggttgaacc gcaggaagag
    27421 acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga acctgaagtt
    27481 gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga aacggtagcc
    27541 gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat gacgcgcgct
    27601 ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg ccctactttt
    27661 gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc ctctgccgct
    27721 cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc cctggtaact
    27781 gccagggctt ttttatttca tgtttgaatc aacatattaa aattcatcaa atggcatcct
    27841 tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc cccggcgatc ccagagcggg
    27901 cagacaaaac atacccagtt tcatgatatg ttttaaagcg agcttatttg ttgagctgga
    27961 agagcgacaa tccctgcata tcggtaaatg ctttatacga tgcctgcaat gccgtttgct
    28021 gcatgatgta agatgaaata gttgcattcc agtccacatc aaccagatcg ctcatctgct
    28081 gcgtttgccc taaagcgcga tcgctaccta aggaatccag cgactccagt tcgttcagct
    28141 gcgtgcctaa ttccgcgcga acggtaagga cattattcag cgagtttttc agtccacggt
    28201 tggttttatc caatgccgca gcggcgattt ctttatccgc ttcgctatcc gcgaccggcg
    28261 ttttcagggc tgcgatggcg ctatccagca tggcaaaaag attggtttca gaagcgctac
    28321 cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa aattttgtcg cccgtatgtc
    28381 ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat actttctgtt ccgccgacat
    28441 aatcaccgtt agcctcacta aacggcgcag tctctgtttt ataaccggca aaaatgtagc
    28501 gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaaac ccctgaatat
    28561 ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg tagacaattt
    28621 tttcctgagc attctggata gcagtggtga cctggctaag tacactctct tccagtgaca
    28681 ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc tgtgcctggg
    28741 agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg actcgcttac
    28801 ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg atgccgcgca
    28861 tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt
    28921 aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata
    28981 ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg
    29041 ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gcgctactgg ttttcaacgt
    29101 cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc
    29161 accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttatc
    29221 gctatcaccc gcatcttctt cgctcgccat cgctattttc gcttcgtcgg tgattaatac
    29281 atccatgttg acgatagcgt cacttactgg tttcagcgtg aagctgtcgt taacagcagg
    29341 cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg catccggcgt
    29401 caccgtaaaa gtggtattac tggcaaggcg ggtgacctgc cactgattat tatcgaacga
    29461 gattttgtaa tcagtcgcca gtaccgcgga ggcatcagtt accgtggcac cgatcgcaac
    29521 gtcacctgtg tttttcgtgt tttgcagaac cgcgggctta ccgatagcaa agaaatcttc
    29581 accagcatcg ccgttggcat caaatccggc tttgtgttgg ctgttgaaag cctcggcaaa
    29641 tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag aacggaatgt
    29701 cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc
    29761 cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag gaactgccgc
    29821 cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggccatcg tgatgttata
    29881 agtaccgcca tcctgaacgc tgacttctac gccaacaatc tggtttaatt cgctcaccag
    29941 ttgatcgcgt tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg
    30001 cgagatttga tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct gatcaacgct
    30061 ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc agatattgat cggtggtttt
    30121 aaactgattc accaaccctt ctgatttccc aatcagcgcc tggcgcgctg ccgggtcttc
    30181 cgcgttactc accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga
    30241 agaggtactg gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcagtcag
    30301 gccgctactt tgcgtctgcg ccgcacgtaa ctggttggta ataaaagcat catactcacg
    30361 ctgcacacca gaaacgtaga caccattgcc aacccagccg ccagcgccca acgtgctatt
    30421 ggcctgcgcc ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt
    30481 attacttgcc gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat
    30541 caagctggac atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc
    30601 cgccggactt gagttattca gaacagatta tcaatgttca tgctgtaggt tttgctcacc
    30661 ttgtcgctta tcgatttcat ctgctggatc atgttggtga gtttacgggc atagtgagga
    30721 tcggtagcat accccgcgtc ctgtagggcc tgcgccccct gctccgcact cgcggcggtc
    30781 gtcacggcgg cgtagcgcgg gttacgtgtt aacaacccaa cgtaatccga caatgcttcc
    30841 agatacgagc tgtagacccg aaatttcgct tttactttct tcgcttcgcc attttcatat
    30901 tcagtcgtgg tgatttcagt gactggccct ttccagttgc cagaggcttt gacaccaaac
    30961 aggttatagc tcggctcgcc gttttcgcgg cggatttgtc gttgccccca gccagattcc
    31021 agcgccgcct gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg
    31081 gcgggcagcg agagttgcgc gaggaatgct ttactgtcac ccggcagcga atcatcgtag
    31141 ttacgtggta cggccttttg caccagctga gaaagcgcct gattttgata acgcaccaca
    31201 gtttcgagcg ggaatttcat cggtgctgct ggcgtgggct cctctggcaa tggttgttct
    31261 ggcgtcatct gtttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt
    31321 tgggcaatct gctggtcata catactggta tacaggcgag tgtgctcgct gctgaacagg
    31381 ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc
    31441 acctggcggg ccaccggacg gatatttgcc gccggatctt cgcccgcttt cgcctttagt
    31501 tcgttgagtg attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc
    31561 agatgatttc cagttttgcc cgcagacatc ccgcactttg cattgattgc aaaatagaca
    31621 tcagatccat cggcgtagcg cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg
    31681 cgctggaacg tacgctttgc agcgaaccgc cgctctggcg taagtcgatc tgcgtttgtg
    31741 gcgttaccac ggtctgtccg ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac
    31801 gattgactgt aactgagaga ttcccctgcg ctaccgcgca gctgtcgagg gtcacttcgc
    31861 gattcatcac caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga
    31921 cattaacctg catattctgg atatcggcaa ggaaacggac ctgggaactg ttgccacttg
    31981 gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc
    32041 ggttgatggt gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat
    32101 taagggtatt cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg
    32161 tgatccgtcc accgttcagt tggttcacct gaacactgct accgccagcg gaggctcctg
    32221 cgccgccaac cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa
    32281 gcggtgtcat caacaacgta cctccacgca agcttttggc attacccatg gaggaaacca
    32341 ccacatcgat ggtttgcccc tgacgtccaa acggaggaag tgacgctgtc accattaccg
    32401 cagcgacgtt ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga
    32461 gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt cgtctggtca ccggttccat
    32521 ccagccccac caccaggcca tagccaatca gtgagttttg ccttaccccc tgaacactgg
    32581 tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag
    32641 agagaaattt aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgttg
    32701 caaccagccc atattttgcg cttcgttaat gtagccattg cctacgtatt caatgcgcgc
    32761 atccgccacc tgagtagacg gtacggtatt gctgccgctg atggtgcgtg gattaaccac
    32821 gccagagaag cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac
    32881 atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt
    32941 attgctggca ttggccccgc cttttccgtt gaacgtgtta ccaccggagg cttcgacatc
    33001 ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt
    33061 agttttaccg tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg
    33121 caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg
    33181 atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggagtcgg
    33241 accgggaacc ggttgtgcac tggtcgctcc ctgtaccagc ggcgtggagg gtatccaggc
    33301 gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt
    33361 ttgcatggct atcatcttca aaaatcaata aaccggtgaa cctgccaccg gttatgcctt
    33421 agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt
    33481 cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg
    33541 tttcaacata cccttgatac agcagtcccg caccgttcag gcccggcgtg ctttcgttcg
    33601 gtgcaccgga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agtccagtat
    33661 cattcataaa ggtggtgaga ttgagctgcc caacctggac cggagctgcc tggccttgtt
    33721 gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg
    33781 tgatcgctgg ctgcacctga aaaccaccag ccgtcaccaa ctgcccgttc tgatccacct
    33841 ggaaagagcc gtcacgggta taggctgatg agccatctgg caacatcact tggaaaaagc
    33901 cctgcccttt aatcgcgaca tctttgctgt tgttggtctg cgacaggttg ccctggctgt
    33961 gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg
    34021 tggtttgttc ggaagactgt gcccccggct gacgaatggt ttgataaagc agatcttcaa
    34081 acaccgcgcg ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga
    34141 cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca
    34201 taaaatgtcc tgtagtggat tagtgacaaa gtgcatatgt cttcttgccg gatgcggcgt
    34261 aaacgcctta tccggcctac aagtgctcgc aaaatcgata aattgcgatt gcccccatag
    34321 acccgataaa cttgcgcatc gggcaactta tacttgtcat gtatcctttc aattaactca
    34381 tcgacagcag ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct
    34441 gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt
    34501 tactgccttc cagaacaccc gacatcacac gcaaggttgg atctgcctgc agtacaggcc
    34561 cacgcgtggc ctgggtttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt
    34621 cgctgcccgt ggctttcacc agcttcagac gccccactgg cgcaaccgta tttgccggat
    34681 cgcccggatt cagcgccgag attgtgccat cggcagctat cgtgatttcc gccccttccg
    34741 gcacagcaat tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg
    34801 tgggatcaac ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca
    34861 cggccagcca accatcctgc tgcaacgcaa cgtccagcgg gcgcgaggtg taatccatct
    34921 tgccgggcgt catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtaggcagtg
    34981 aaagcccttc caccggcact gcgcgcaagg cgtttaactg cgcacgaaaa ccgggcgtag
    35041 aggcattggc cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgctc
    35101 ccatcgcggt ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta
    35161 accagcgtgt tgaggatctg gtcctgggtt ttaatggtct gggcgttaga ctgatagtta
    35221 cgctgggcaa cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccagc
    35281 gcaccgttgg tcagggtacc aaagttgccc gtcccggctg tccccaacag cgccacgcca
    35341 gaagattgcg tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca
    35401 aagttcgcca gtacaatctg ccccagcagt tgggtttgtt cgttggaata gttgccgaca
    35461 accgtaccgt catcattgat ttgataactc accagatcgc ccggtttgta gccgttctgg
    35521 gtggttgcca caatgttgtt agcgccggta ttttgctgca tggagttgag gaagctcagg
    35581 ctaaacgtgg cgggatctgc gccgttaatt gcgccagtgg taatattctc tgttggattt
    35641 gaggtcagaa cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt
    35701 ggatcactgc tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtag
    35761 acgctcatgt catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta
    35821 tagctatccg cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc
    35881 aggttgatct gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc
    35941 gaaatgttag tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa
    36001 ccggtcagct gtaaaccttg catattcacc aggttacggt tttcatccag cttaaattgt
    36061 ccgttacggc tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg
    36121 ctgatagcaa cgtccagacc acgcccggtg ttggtggtcg tgccatcggt aaagtcctga
    36181 gtgataccgg caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa
    36241 gaggccgtgc ctgatttaaa gccgtatgtg gcggagttgg cgatattgtt gccaataaca
    36301 tcgaggttgg tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac
    36361 tcctgaaatg tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa
    36421 gtgccgagat ccagcgtatt accgttgttg ccgcggatca caccctgcac cagagcaaac
    36481 tgcagcggct gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacgttgtaa
    36541 gaaccgttcg gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtggact
    36601 ccggcggtca gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg
    36661 gtggccgtaa ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc
    36721 acagcccctt cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg
    36781 tgaccaatca ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat
    36841 ccgagcgtgg tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac
    36901 gtcagctcgt tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc
    36961 aaagtcagaa aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg
    37021 gtggtactga cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc
    37081 tcctttattg accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt
    37141 caacattggc ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat
    37201 caacgtttgg cattttcacg tagccctttg catccgccag cggattaccc ggttcataaa
    37261 ccagtttgtc cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag
    37321 cacctggtgc agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcag
    37381 gaccggtcac gctatcagca ttcgccagat tactggccgc cacgttcagg cgctgggact
    37441 gggcagttaa cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc
    37501 cgctctgtaa aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct
    37561 ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac
    37621 cgtcaagcga aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg
    37681 cctgcgccgg aatgtgttgc gttgaggtca tcgtcagtgc aaccacactg gttgcatccc
    37741 gtccacgttg catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac
    37801 caggggtatc agcattggcg atgtttgctg ccagcacttc ctgacgctgt gcgcgcagat
    37861 tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc
    37921 aggtatcaaa attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa
    37981 acgcaaaatg ggtcgctatt tatgccgttg atgaccattg cggacaggta caattcacgt
    38041 tgtagaaatg gctgggggcg aaaatgctgg caataaaacg tagcgtggcg atcatcgcga
    38101 tactgttcag tccgttaagt gcggcgagta atctcacatc gcaattgcaa acctttttta
    38161 gcgcccagct cgcagggata agtgatgagg ttcgtgtttc tattcgtaca gcgcccagtc
    38221 tactgccgcc gtgcgagcag ccattgcttt cgatgagcaa taattcccgc ctgtggggca
    38281 atgtgaatgt gttggctcgc tgcggtaacg acaaacgata tttacaggtt aatgtacagg
    38341 ccacaggaaa ttatgtggtt gccgcgatgc ccattgtgcg gggaggaaag ctggaagctg
    38401 gcaatgtcaa actgaaacgc ggacggctgg ataccctgcc accgcgtacg gtgctggata
    38461 tcaatcaact tgttgatgcc attagcctgc gcgatctatc acccgatcaa cctatccagt
    38521 taacccagtt tcgccaggca tggcgggtaa aagcgggaca acgcgtcaat gtgatcgcca
    38581 gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct gaacaatgca gccgtcgcac
    38641 agaatgcgcg ggtgcgcatg gtatcgggac aggtagtcag cggcgttgtt gatgcagatg
    38701 ggaatattct tataaacctg taagctgtta aagattaccc gtcccttgcc gataaataag
    38761 caacacatga taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc
    38821 ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaaca
    38881 gccgggcggc aaaaacaacc gcctccacca gcaccagtgt gacgttaagc gacgcgcaag
    38941 caaaactgat gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac
    39001 tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa aattgccgat gcgctgatca
    39061 acgaagcgca gcaagacttg cagagtaact gatcgtatga cacgtcttgc agagatcctc
    39121 gaccagatgt ccgctgtgct taacgatctc aaaacggtaa tggatcaaga acagcaacat
    39181 ctctctatgg ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca
    39241 ctgctggcga cgctggatta cctcgaacag ttacgcagga aagaacccaa tacagcaaat
    39301 agcgctgata ttagtcaacg ctggcaggaa attactgtga aaacgcagca actacgccaa
    39361 atgaatcaac ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg
    39421 gaaatgttga aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca
    39481 acccatcgcg gcagtaaaaa gatttcgatc tgaagattat ctccggcctg cactgcaggc
    39541 cggaatgcat tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc
    39601 cagtgcagcg aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagcaagcg
    39661 ccagggcatg gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc
    39721 agacatcacc agtaccgcca ccaccagacg caacagaaac gccatccagc caggttgcgg
    39781 ggtaaagatt ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc
    39841 cagaccaata gaaagtgaca gcccggcatg tttcaacgga ccgataaacg ccaggttcat
    39901 caattgcgtt aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg
    39961 ggaataaaag ccaggagcca acactttcac tacaatcagg ccaatcaaac ccaccgagta
    40021 ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa
    40081 cagcgaaacg gtcaacggac ctgaaagaat gcccaacgca accgcactcg gcagcgccaa
    40141 caggaaacaa agacgcagcc cccagtccat caaacggttg tattcatcat gattgccact
    40201 ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca agcgccacgc ccagcacacc
    40261 ggacggaaac tccattaagc ggtcggcgta atacatccaa gacaccgaac cggaagcaag
    40321 aaacgaggca aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat
    40381 cgccggtccc atctgtttca ccacgcgcat tgctccggca tcgtggaagt taatgcgcgg
    40441 caggaccagc atgccgatct tcttcaggtg cggtagctga tacaccagct gcaggacgcc
    40501 gccgaccgta acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa
    40561 cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc gcaaacgccg gaatcgagaa
    40621 gcggttccac gtattcagaa tcgctcccac cagcgacgcc agggagatca gcaagatata
    40681 gggaaaggta atctttagta gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa
    40741 gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac
    40801 cgccagcgca agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcgtcttc
    40861 accctgctta cttttatatt ccgccagaat cggtacaaat gcctgggaaa atgccccttc
    40921 ggcaaagata cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc
    40981 catccctgcg ccaaagattc tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga
    41041 aaacatggtc atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat
    41101 ccagacccac gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg
    41161 gattatggtg aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc
    41221 agatcttgtc aacgatacgt tgcgccagca ctgcctgttc gccggcggtt tgcggaactg
    41281 tctgattttg cacacattca atgaagtgcc gcgcacagcc gacaaatcca cgttgttcaa
    41341 gcgtactctg ccaaccagga atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc
    41401 attcgcgcat atccgtaatg tcgatgagcg caccgtcagt cacggcctgc acggtttcac
    41461 gctgacttcc ggcacggcga tgcatacagg tggtgatctg caaaggaccg gccgaaaaat
    41521 ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag tagcgtaccg ccatccagag
    41581 aggctttgcc gcccgacaac cacagcgcgg tatccaccac atgcagataa tcatccagca
    41641 acgtgaaata aagatcgtgt ggcccgacgc tattgctacg atgtttatcc attcttagcg
    41701 aggctgcggt ggcgagttgc gtttttaact caccgtagag tggtgcgaaa cgacggttaa
    41761 aaccgaccat caacgtcagt tttttccgcg ccgccagttc caccagccgt tcagcatcgc
    41821 gcagattttc tgccagcggt ttatcgacac agacatgtac ccccgcattg agtaacgtac
    41881 tgaccacgtc aaagtgactg gcggtgctgg aatgtacaaa aaccgcatcg caactggcgg
    41941 caaggctgga taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt
    42001 tcgcgcgcgt aggcgaccag gctccttgta acgtccagtc agacgctgcc gccagcaccg
    42061 gtaaccacgc tttttgagca atgccgccta atcccactac gccgatacgt aattttttca
    42121 ctttaatctc ccaggtgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg
    42181 atttccaggg cttcgacgcg ggcctgtaaa tcaccgtcaa ccgcgctcga catatccgtt
    42241 accgacggct gatcctctac ctcaccgctg aaaagatgca tgtagcggct ttcacgttta
    42301 cccggttcgc gggccagacg caccacaaaa ggaccatctt cgcgattcgc cagttgttcc
    42361 agcgtcgact ccacttccgc catatcgctg aattcataca ttcgcgcggc gcggctgcgc
    42421 agttcacccg gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttctgcg
    42481 gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac gttgctcata tttggtgacc
    42541 cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt tgaccagatt atccagctgt
    42601 tcctgcactt cagattcact cagattcatg accggttcac ggttcgtttt ctgattacag
    42661 gccgtgacta caccattgac tgagagcggg tattgctccg gcgtggtcac ctgcttttcc
    42721 agcaagcaac caatcacacg agcttccagg gcagtaagtt gatatttcat gagattctcc
    42781 ttagcggccg ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg
    42841 tccatcaatc aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag
    42901 taaatcaccg ctgcgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg
    42961 ggtgcgctgc atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg
    43021 ccatttttgc ccaatcgaat aaccgagata gcaggcatga aaagagccgc gaacaacatt
    43081 ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg tcgaataaac caaagtagaa
    43141 agctgaacct tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg
    43201 atagcagtgg ctttcgtcgc gcactggctc ccagggcttg aggaaatggc gattctctgc
    43261 gtaataatcc gccagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc
    43321 tgtggttaag cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct
    43381 tcccgtaacg tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga
    43441 aaagtccaaa atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag
    43501 cgacgttttg ataaaaaaat attgtccccg aagtgatggg aaaagtctta gcggcaatgc
    43561 agaatagaaa gtgctcatct tttatttccc gggaggggaa atgtcccgcg tatcgcaggc
    43621 gaggaacctg ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt
    43681 tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat
    43741 ggtcggtatt gctctcggtc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg
    43801 cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc
    43861 cgccggattc gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct
    43921 gctctcggga ctcggcggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact
    43981 aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg atgatgcagg acagtgtcgg
    44041 tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg
    44101 cgccacaggg gccgttttgt ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc
    44161 atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga
    44221 caagcgtttt gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt
    44281 gatgctgatg ctgccaatta tggtcaacga cgtggctggc gcaccctctg ccgttaaatg
    44341 gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag
    44401 tgaaaagcat tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag
    44461 catgatgccg gtgggcatgg tcagcggcct gcaacaactt ttcaccctga tttgtctgtt
    44521 ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga
    44581 cgcaagagct cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggcggcgc
    44641 tattggttat atcggtggtg gctggctgtt tgacctgggc aaatcggcgc accagccaga
    44701 gcttccgtgg atgatgctgg gaattattgg catcttcact ttccttgcgc tgggttggca
    44761 gtttagtcag aaacgcgccg cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc
    44821 cccctgactc ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct
    44881 atacatttac gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa
    44941 tatccccgtc gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt
    45001 cggtcaaaaa caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat
    45061 ggatatcgtt cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc
    45121 ccctgccatt gaagagtggc tgcgcaaggt caatggctac gccaacaaac tgctgttgcc
    45181 gcgttttgcc aaatcggcat ttgatgagtt ttctactccc gccgcgcgca aatatttcgt
    45241 cgacaagaaa gaggccagcg cgggtaattt tgccgacctg ctggcccact ctgacggtct
    45301 gattaagaat atcagcgatg atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc
    45361 cgtgaatggc gaactttcgg aagatgatat tcagctattt ccgctactgc gtaatctgac
    45421 gctggtagcc ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa
    45481 acagacacaa atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc
    45541 ctgtattcgc gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga
    45601 cacatgctgc gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca
    45661 agtttttatt tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca
    45721 cccaatacac catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct
    45781 caaaagatat cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacttgacca
    45841 gccaaattgg tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca
    45901 tgaactccct gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg
    45961 tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg
    46021 tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat
    46081 tacgcaatta ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg
    46141 aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc
    46201 cgtttacgga ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac
    46261 gtttccgctt atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa
    46321 tgactgcacc atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc
    46381 gcgatggcga catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta
    46441 tcgtaatgcc caatctggct ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc
    46501 aacgtattct tgatgccgta cctgccgggc acaatttcac cccattgatg acctgttatt
    46561 taacagattc gctggatcct aatgagctgg agcgcggatt taacgaaggc gtgttcaccg
    46621 ctgcaaaact ttatccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg
    46681 acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg
    46741 gtgaagtgac acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg
    46801 tgatggaacc tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca
    46861 ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc
    46921 cgcagcatct gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt
    46981 attgtttacc catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca
    47041 gcggttttaa tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag
    47101 agagcagttg cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta
    47161 ccgtctttga agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc
    47221 cgcagttcta tggcttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac
    47281 aggttgctga aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa
    47341 cggtacgctg gtccgttaaa caataaaaat tgtagcttcc tgttgtcatt aggttatttt
    47401 acctgtataa ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata
    47461 gcgaaaactt ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc
    47521 cgtattcagt atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg
    47581 aataatttat cggttattgg cgcaacaaaa gaagataaac agcgcattag cgaaattctc
    47641 caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt
    47701 gtttttgccg ggtcgccccg gcttttttat tttacttaca aattttg
//
LOCUS       DAPHJE010000021        47032 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPHJE010000021 DAPHJE010000000
VERSION     DAPHJE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.021"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(255..1505)
                     /locus_tag="RH222_001217"
     CDS             complement(255..1505)
                     /locus_tag="RH222_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV3796271.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1599..2537)
                     /gene="psuG"
                     /locus_tag="RH222_001218"
     CDS             complement(1599..2537)
                     /gene="psuG"
                     /locus_tag="RH222_001218"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV3796272.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(2525..3466)
                     /locus_tag="RH222_001219"
     CDS             complement(2525..3466)
                     /locus_tag="RH222_001219"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV3796273.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(3890..5581)
                     /gene="fruA"
                     /locus_tag="RH222_001220"
     CDS             complement(3890..5581)
                     /gene="fruA"
                     /locus_tag="RH222_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV3796274.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(5598..6536)
                     /gene="fruK"
                     /locus_tag="RH222_001221"
     CDS             complement(5598..6536)
                     /gene="fruK"
                     /locus_tag="RH222_001221"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV3796275.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(6536..7666)
                     /gene="fruB"
                     /locus_tag="RH222_001222"
     CDS             complement(6536..7666)
                     /gene="fruB"
                     /locus_tag="RH222_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV3796276.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGGDAEQALKAIGDAIAAGLGEGA"
     gene            8034..9215
                     /gene="setB"
                     /locus_tag="RH222_001223"
     CDS             8034..9215
                     /gene="setB"
                     /locus_tag="RH222_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV3796277.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(9212..9466)
                     /locus_tag="RH222_001224"
     CDS             complement(9212..9466)
                     /locus_tag="RH222_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3796278.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            9621..10193
                     /gene="yeiP"
                     /locus_tag="RH222_001225"
     CDS             9621..10193
                     /gene="yeiP"
                     /locus_tag="RH222_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV3796279.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            10416..11882
                     /locus_tag="RH222_001226"
     CDS             10416..11882
                     /locus_tag="RH222_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV3796280.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            12000..12986
                     /gene="yeiR"
                     /locus_tag="RH222_001227"
     CDS             12000..12986
                     /gene="yeiR"
                     /locus_tag="RH222_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV3796281.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            13025..13738
                     /gene="lpxT"
                     /locus_tag="RH222_001228"
     CDS             13025..13738
                     /gene="lpxT"
                     /locus_tag="RH222_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV3796282.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            14150..14716
                     /gene="mepS"
                     /locus_tag="RH222_001229"
     CDS             14150..14716
                     /gene="mepS"
                     /locus_tag="RH222_001229"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV3796283.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            14897..16453
                     /locus_tag="RH222_001230"
     CDS             14897..16453
                     /locus_tag="RH222_001230"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV3796284.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHRHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            16535..18349
                     /locus_tag="RH222_001231"
     CDS             16535..18349
                     /locus_tag="RH222_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3796285.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            18350..19444
                     /locus_tag="RH222_001232"
     CDS             18350..19444
                     /locus_tag="RH222_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV3796286.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            19444..20469
                     /locus_tag="RH222_001233"
     CDS             19444..20469
                     /locus_tag="RH222_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3796287.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            20471..22060
                     /gene="yejF"
                     /locus_tag="RH222_001234"
     CDS             20471..22060
                     /gene="yejF"
                     /locus_tag="RH222_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV3796288.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(22064..22408)
                     /locus_tag="RH222_001235"
     CDS             complement(22064..22408)
                     /locus_tag="RH222_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796289.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(22741..23931)
                     /gene="bcr"
                     /locus_tag="RH222_001236"
     CDS             complement(22741..23931)
                     /gene="bcr"
                     /locus_tag="RH222_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV3796290.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(23959..24654)
                     /gene="rsuA"
                     /locus_tag="RH222_001237"
     CDS             complement(23959..24654)
                     /gene="rsuA"
                     /locus_tag="RH222_001237"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV3796291.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            24804..26564
                     /locus_tag="RH222_001238"
     CDS             24804..26564
                     /locus_tag="RH222_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3796292.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            26689..26973
                     /gene="rplY"
                     /locus_tag="RH222_001239"
     CDS             26689..26973
                     /gene="rplY"
                     /locus_tag="RH222_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV3796293.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(27112..28119)
                     /gene="yejK"
                     /locus_tag="RH222_001240"
     CDS             complement(27112..28119)
                     /gene="yejK"
                     /locus_tag="RH222_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV3796294.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAQAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            28301..28528
                     /locus_tag="RH222_001241"
     CDS             28301..28528
                     /locus_tag="RH222_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV3796295.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            28548..30308
                     /gene="pbgA"
                     /locus_tag="RH222_001242"
     CDS             28548..30308
                     /gene="pbgA"
                     /locus_tag="RH222_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV3796296.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            30383..30459
                     /locus_tag="RH222_001243"
     tRNA            30383..30459
                     /locus_tag="RH222_001243"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30417..30419,aa:Pro,seq:ggg)
     gene            complement(30561..32930)
                     /locus_tag="RH222_001244"
     CDS             complement(30561..32930)
                     /locus_tag="RH222_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3796297.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAI
                     DTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANV
                     WAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSL
                     ENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNT
                     LSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDA
                     AFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAE
                     LDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREE
                     SSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTI
                     HGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGM
                     RADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNN
                     DVAGARGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
     gene            33250..33897
                     /gene="narP"
                     /locus_tag="RH222_001245"
     CDS             33250..33897
                     /gene="narP"
                     /locus_tag="RH222_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV3796298.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(33932..34984)
                     /gene="ccmH"
                     /locus_tag="RH222_001246"
     CDS             complement(33932..34984)
                     /gene="ccmH"
                     /locus_tag="RH222_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV3796299.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(34981..35538)
                     /gene="dsbE"
                     /locus_tag="RH222_001247"
     CDS             complement(34981..35538)
                     /gene="dsbE"
                     /locus_tag="RH222_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV3796300.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(35535..37478)
                     /gene="ccmF"
                     /locus_tag="RH222_001248"
     CDS             complement(35535..37478)
                     /gene="ccmF"
                     /locus_tag="RH222_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV3796301.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(37475..37954)
                     /gene="ccmE"
                     /locus_tag="RH222_001249"
     CDS             complement(37475..37954)
                     /gene="ccmE"
                     /locus_tag="RH222_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV3796302.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(37951..38160)
                     /gene="ccmD"
                     /locus_tag="RH222_001250"
     CDS             complement(37951..38160)
                     /gene="ccmD"
                     /locus_tag="RH222_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV3796303.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(38157..38894)
                     /gene="ccmC"
                     /locus_tag="RH222_001251"
     CDS             complement(38157..38894)
                     /gene="ccmC"
                     /locus_tag="RH222_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV3796304.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(38936..39598)
                     /gene="ccmB"
                     /locus_tag="RH222_001252"
     CDS             complement(38936..39598)
                     /gene="ccmB"
                     /locus_tag="RH222_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV3796305.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(39595..40212)
                     /gene="ccmA"
                     /locus_tag="RH222_001253"
     CDS             complement(39595..40212)
                     /gene="ccmA"
                     /locus_tag="RH222_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV3796306.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(40231..40833)
                     /gene="napC"
                     /locus_tag="RH222_001254"
     CDS             complement(40231..40833)
                     /gene="napC"
                     /locus_tag="RH222_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV3796307.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(40843..41292)
                     /gene="napB"
                     /locus_tag="RH222_001255"
     CDS             complement(40843..41292)
                     /gene="napB"
                     /locus_tag="RH222_001255"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV3796308.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(41289..42152)
                     /gene="napH"
                     /locus_tag="RH222_001256"
     CDS             complement(41289..42152)
                     /gene="napH"
                     /locus_tag="RH222_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV3796309.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(42139..42834)
                     /gene="napG"
                     /locus_tag="RH222_001257"
     CDS             complement(42139..42834)
                     /gene="napG"
                     /locus_tag="RH222_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV3796310.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(42841..45327)
                     /gene="napA"
                     /locus_tag="RH222_001258"
     CDS             complement(42841..45327)
                     /gene="napA"
                     /locus_tag="RH222_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDV3796311.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(45324..45587)
                     /gene="napD"
                     /locus_tag="RH222_001259"
     CDS             complement(45324..45587)
                     /gene="napD"
                     /locus_tag="RH222_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV3796312.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(45577..46071)
                     /gene="napF"
                     /locus_tag="RH222_001260"
     CDS             complement(45577..46071)
                     /gene="napF"
                     /locus_tag="RH222_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV3796313.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            46180..46344
                     /locus_tag="RH222_001261"
     CDS             46180..46344
                     /locus_tag="RH222_001261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796314.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            46479..46967
                     /gene="eco"
                     /locus_tag="RH222_001262"
     CDS             46479..46967
                     /gene="eco"
                     /locus_tag="RH222_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV3796315.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT        11471 a        12759 c        11608 g        11194 t
ORIGIN      
        1 gtcaggactg gcgctggaga tggacccgga gaataaattc tccgggatga tgcagtaaaa
       61 aaataatctg gaatatactc taaataattc gagttgcagg aaggcggcaa gcgagtgaat
      121 ccccaggagc ttacataagt aagtgactgg ggtgagcgaa cgcagccgca gcacatgcaa
      181 cttgaagtat gacgagtata gtacgcgatt gccggatgca acgcttaata agcgagtaca
      241 tccggcaatg aaactcaagc taaaccaata aagaacccgg caatggtcgc gctcatcagg
      301 ttggaaagcg tcgccgccgc cagcgcccgt aaaccaagct gggcgatttc cggcgcgcgt
      361 tttggcgaaa tagccgaaaa cgctccaacg acaacaccga tagaaccaaa gtttgcaaac
      421 ccacagaggg cgaacgagat aatggcaatg gtcttgacat ccagcgtgcc gctcgtttgc
      481 agatagggcg agaaattgag ataagcaaca aactcattaa tcgccagttt ctgcccaatc
      541 aaacttccgg caagatttgc atcactccag tccaccccca taatccatgc caacggggcc
      601 agcacatagc cgaaaatgct ttccagagag acattggcaa aaccaaacca gccgccaact
      661 ccaccaataa tgccgttgat cagcgcaatg atcgccacaa atgccattac caccgtcgcc
      721 acaccggcgg cgattttcag cccggtcatt gcaccgttcg ccgcagcttc aataatgctt
      781 tttggcggtg tttcgctaaa cgacagattt tcaaacgtaa cttgcgaagg ttccgtcgcc
      841 ggactaagaa tacgtgcaaa caggataccg ccaggaatag ccatcagcga agccgctaac
      901 aggtagtcaa ttggtacgcc cattccggca taaccaatca tcatcgaacc agcaatggac
      961 gccatcccgc tacaaattgc ggtaaacaac tcgttgcgat tcatgcgatc gataaacggt
     1021 ttaacgatcg ccgggatctc attttgcccg aggaaaatag ttgtgactgc gacaaaagat
     1081 tcgattttgc tgatgttaag agctttctgg aaaatgctgc caaggatgcg aatcagcagc
     1141 cccatcacgc caatgtagta cagcagactg atgagcgcag taacgaaaat aatcgccgga
     1201 agtacgcgaa aggcgaagat aaaacccgca ccgtcaaaca ggacatccat tttcggccca
     1261 accagcgaac caaaaatgaa ggcgctaccg gcatcactgt aagacatcac tttatgaacg
     1321 cctaatgccg cctgttctac tgcccacttt cccggtggga agtagagcat gatgccacca
     1381 atagcgattt gcagcagcag tgcggctcca accgtgcgca aactgatgct ctttttattc
     1441 actgacaaca gaaatgctat tgccagtaac accaccatcc ccacaacact tctcattata
     1501 tccataatga ttttcccttc atgccagtaa acccggcgtc agcgccaggt tttggtatgc
     1561 ttgatgagta cgggcgacgg ctttctgccc gtcagaaatt aacccgcgag acgctggtat
     1621 tctttggcaa tttcactcgc cagaatggcg ttgttgaaca ccagctggat attggatttc
     1681 aggctgtcac cgccggtcag ctcagcaacg cgcgccagca ggaacggtgt actttcttta
     1741 ccaataacgc cctgcgcttc agcttcagct accgcctgat cgatcgccgc attgatagtg
     1801 tgttccggca tcgcaaactg ttccgggatc gggttcgcta ccacgaggcc accgttcagc
     1861 ccgctttgcc acttcaccgc cattgcacgg gcaatttcac tggcgctgtc gagacgaatg
     1921 ctgacgtcaa acgggctggt acggcagaaa aacgcaggca gcgcttttgt ctgatagcca
     1981 attaacggca caccgaaggt ttctaaatat tctgttgtta atcctaaatc gagaatagat
     2041 ttcgccccgg cacaaacaac ggtgacatta gtatttgcca gttcttgcaa atcggcagaa
     2101 atatcgaagg tatgttccgc cccgcgatgc acaccaccaa ttcccccggt ggcaaatact
     2161 ttaattccgg caagcgccgc aataatcatc gttgaagcca cagtggttgc gccatttttt
     2221 ccggcggcaa caacaaaagg taaatcgcga cgactaactt tggtcacgtt atgcccttca
     2281 cgacccagta attcaatttc ttctttgctt aaacccactt tcatcacgcc gccaataatg
     2341 gcgatagtgg caggtactgc gccctgttta cgaatagttt cttctacttc aattgcggtc
     2401 tgggcatttt gtgggaacgg catcccgtga gaaataatgg tcgattccag cgccacaacc
     2461 ggttttttgt tttttaaagc gtcctgcact tccggggaaa tttgtaataa ttcaggggaa
     2521 atttttaatt cagacattct gcgttctcca ctaacgatat aacgttggca atcgataaat
     2581 cggggttatt ggtgtattca caggagagcg ccattgacga acatccctgt gcgaaacgaa
     2641 cagattcggc aaacggcatt ccgtctaccc aacacgaagc aagtcccgcc atcatggcat
     2701 cgcccgctcc ggtaacatta ataacattgg ttttgatcgg cgcagaccag ccattttcac
     2761 cgctgatatc gctgtaataa acgccgtcgc cgcccatgct caataccagt cggttcaggc
     2821 catgttgatg gaaccaggca gcaacttttg ccacatcgtc acgccctgac agcgcaatcc
     2881 cactcagggt ttccgcttca aggcggtttg gcttgagagt gtggatctga tttagacggt
     2941 cgcgcacttt gacacatttc cacgctgaaa ccggatcgac aaatacgggt acgttggcgg
     3001 cattatccag aatccatgcc agtgcctctt cactgatatt acagtctgcg acaatgacct
     3061 ttgccctctg aataaattca cggtgctgtg cgagatattc agctgtaata gcgttgctaa
     3121 tattcatgtc atttatagca accagcattt caccggtatt atcgagtaat gataaatagc
     3181 ttgacgtatt ttctcctggc acaatcaggc atttatcgac ataaacgcca gattgattgg
     3241 tttgcgttag cagcgattga ccataaaagt cactgcctac ggcgctcagt agccaggctt
     3301 tgttccccag caacgccagg ttttgtgcaa tattgcgccc gactccacca ggcgtaaatt
     3361 ttattttacc tggatttgaa tccgcataat ttaatgattc atgtgaatat ccggcgacat
     3421 caatattcgc cgagcctata attacgacat aatccttttc gcgcatagca cgtcctctgg
     3481 taaaaaatta ccaaccctgt caatccacaa gaatatagat actgcaaaga cggtttaaac
     3541 atgtgttcac atttaaacat gtgctcatag tatgcatgtt ttacgtaaag taaattaccg
     3601 ccagagaggc taaatgggca gatttgagca ggctcacaaa atgcgattaa atgcctgaat
     3661 tacaaaagcg aggaatttgt gacgggatgc acagaacagg aacaataaaa aagggcagaa
     3721 aataatctgc ccttgaggaa ataaccttag tgtttgatga acgtttcaag tttctactga
     3781 aagataaaac catttaaatt cataaaatta cattcacaag ctggtcattc cctttgcgct
     3841 catccaatga tacagggcag gaaatttctg ccctgtaaca caccttttat tacgctgctt
     3901 tcgctactgc gtccacttcc ggacgtttca ggaaggcata ggccaaaccc gccaccagcg
     3961 taccggcaat aattgctacc aggtaaccca gtaccggcgt aatagcgcca gggatcagca
     4021 gaacaaacag accaccgtgt ggtgccatca gtttcgcacc aatcgccatt gagattgcgc
     4081 cagtcagcgc accacccacg atacagcacg gtagcacacg catcggatca cgagcagcaa
     4141 acggaattgc accttccgaa atgaagcaca gtcccaatac cagagcggct ttgccacctt
     4201 cctgctgcgc tttgtcgaat ttgcgacgcg ccaccattgt tgccagaccc attgccagcg
     4261 gtggcaccat acctgccgcc ataatcgccg ccatcgggcc ataggtttga gtactcagca
     4321 gacccacacc gaatgcgtac gctgctttgt ttaccggacc gcccatgtca gtacacatca
     4381 tgccaccgag gatcgccccc agcagaaccg cattcgcagt ccccatggtc tgcagccagt
     4441 gagtcagccc ttcgagaatg ccagcaaccg gtttaccgat caggtagatc atcgccagac
     4501 cgaccaccag actggaaatt agcggaatga tcaggattgg tttcagcgcc tccatactct
     4561 gtggcagttt cagttgcgta ctgattaact tcgcaatgta accagccagg aagcccgcaa
     4621 taataccgcc aatgaagcca gaaccggtgc tgacagccag cataccgcca atcagccccg
     4681 gcgtgaggcc cggacgatcg gcaatggaga aggcaatata acctgccagt accggcacca
     4741 tcagcgcaaa ggctgaacca ccgccaatct gcatcagcgc cgctgccaac gtacccggct
     4801 ctttaaacgc ttcgatacca aaagcaaaag aaagcgcgat acacagacca cctgcaacaa
     4861 ccatcggcag catgtaagag acgcccgtca gcaagtgacg gtaagcgcct gcactctctt
     4921 tcttaccttc agtggtcgct gtttgagctt tgcccgctgg ttcatacggc gttgcttcag
     4981 caaccgcttt atccagttcc tgcgcggttt tcttcagcgc cagaccggta gaggtacgat
     5041 acatcggttt accagcaaat ttcgccagat ccacttcgat atctgccgcc acaatcacca
     5101 gatccgctgc ggctacttcc tcgggagtga ttgcattacc cgcgccaaca gaaccacggg
     5161 tttcaacttt cacccaccag ccacgttttt tcgcttcggt ttcaatggct tcagccgcca
     5221 taaaggtgtg tgctacgcca gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc
     5281 tggcggcaac cggtgctgtc gcagtgaccg gcgcagtgta aggtttcgca tggcctttgg
     5341 cttcactcag gaacagctca gggtgcgcaa ctgcccggga aatatcaccc agccagacat
     5401 ttttaccgtt cagcgcgctg tcgttcggga tggaatcacc gagaacaatc gccatttcag
     5461 cgtcgttcgg attgtcgatg atttccagtt ttgcttttcg cgccgccgcg cccagcaggg
     5521 tcttcgccat ataggcgcgt gcctgaccga gattagcgtc aataatcagc agcgttttca
     5581 ttatgcctct cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc
     5641 aactgcggac gatcggtaat acccacattg ctttgactta ccgccagggc tgcaacagct
     5701 gtcgccagac gcagtgtgtg ttcactggat tcacgcatca gcaagccata aatcaggcca
     5761 ccaaccatag aatcccctgc gccaacggtg cttacgacat cgactgacgg tggtttggcg
     5821 atccattcgc cggaggcatt aacccaaagc gcgccttcgg cacccagtga aataacaaca
     5881 tgcgcgatgc cttgttcacg tagcgcatgt gcagcttcaa tcacatcttt catttcaggc
     5941 agtttacggc ctgcccagat ttccagctcg cggcggttag gtttcaccag ccacggtgcc
     6001 gctttcaaac ctgctactaa cgcttcacgg ctactatcaa agataatgca aggacactga
     6061 ctacgcaggc gagtcatcca gtcggtgaac gcttccgggc tgacgcctga cggtaagctt
     6121 ccgctgacac agaccatatc gaactgaccg agccagctca gagaatcagt cacaaagcgt
     6181 tcccagtcgg cgggggtgac ttcaaaaccc gagaagttga agtcggtcac ttcaccgtct
     6241 ttttccgtca gcttaacgtt aattcgggtg cgcccctgta caacctggaa acggttggca
     6301 atgcccagct cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc gaggaagccg
     6361 ccaacggtga catcaattcc caggtctttt aatactttgg ccacgttgat gcctttaccc
     6421 gccgcatgca gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag
     6481 aaaccaacaa ggtcataagc cggattaagg gtgatagtag caacacgtct gctcattatg
     6541 cgccctcccc aagaccagca gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat
     6601 ctccaccctg ggcggtaaag cgtaggcgat gacctttctt aacgccaagt gccacaactt
     6661 tcatcagact acgtccgttt gccggtttgc cggtgccatc aaggtttgtc acggtaatat
     6721 cactgttaaa ttgtttaatg gtattgacca gcatggtacc tggacgagca tgcaggccgt
     6781 gttcattgcg caccacaaac tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg
     6841 tcagcagtgc cagcaacgtt gccgcatccg ctttcagcaa gcggtcagct ttattgtcga
     6901 gcaacaaatc agcgagacgc ttaagaaccg cgatgggctg atcgtcattc atcgctacac
     6961 tcaccagcat ggctgccgtt tcgccgtcca catcaaaagc atttgccgca cggcttaccg
     7021 caatcgcgct acgcagattg ccttcggcgc tatcgctcag ccagatgccc tgtccgaggt
     7081 tcagcggttg ttcattgatg gctttggtga cgaaagtggc gtcaactgcc cccgcctctt
     7141 tcagacgcgc agcgttcagc gcctgaagag tcagcagatc gctggcgacg atatccagtg
     7201 tcagcatttc gttgtcgagc ttcagctgct cactctgctt ttcgcccatc agcaatgcgc
     7261 gaagttcttc tgctgttgtt gctgacttca gttgttcagc aacggaatca tcactcagta
     7321 cgtgggtcag ctggcgtagc aggcccagat gttcatccga gctggcagca ataccgatcg
     7381 ccacgtacgc tacctgaccg tcaccccagg tgacgccttc cgggaactga aatacctgaa
     7441 cgccggtttt cagcacctga tcgcgggtgt cggtggtgcc gtgtggaata gcaataccat
     7501 tgccgaggaa cgttgaggtt tgctgttcgc gcgccagcat gccattgacg tagccttctg
     7561 ctacgttacc ggcctgcacc agcgccgcag cgacctggcg aatcgcctct tctttgtctc
     7621 cggccttttc gcccggatgg atgtcctgta cggataactg gaacatagtt ctcctctctt
     7681 gctgaattga aacgattcag cctctatgag aaaaaaagcg ccaacctggc ttagggttaa
     7741 agacaagatc gcgctgaaac gtttcaagaa agcataatac ttctgattca gcacgcaagg
     7801 aaagccgtaa agttatgagc aaaagtttga tgtgctgcac aattttttgt catttttcct
     7861 aattgctgat gggaaaatcc gttatcagca atttcatttc agcagcgtca gcagccaatc
     7921 agcaacagtg aaatggcatt ttgattattc gtggcgcttt tgatttcgct gtctgtttat
     7981 tttctgacaa gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc agcatgcata
     8041 actcccccgc agtctccagc gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg
     8101 ttgcctttct caccggtatt gcgggcgctc tgcaaacccc gacactcagt atttttctta
     8161 ctgatgaagt acatgcccgt ccggcgatgg tgggattctt ctttaccggc agcgctgtca
     8221 ttgggattct ggtaagtcag tttctcgccg ggcgctctga taagcgcggc gatcgcaaat
     8281 cgctgattgt cttttgctgc ctgttaggcg tgctggcctg cacccttttt gcctggaatc
     8341 gcaactactt tgttttgcta ttcgttggcg tctttcttag cagctttggc tcgaccgcta
     8401 acccgcaaat gtttgccctt gcccgtgaac atgccgacaa aaccggacgt gaagcggtga
     8461 tgttcagctc ttttttacgc gctcaggttt cactggcatg ggtcattggc ccaccgctgg
     8521 cttatgcctt agcgatgggt ttcagcttta cggtaatgta tctgagcgca gcggtagcat
     8581 ttattgtttg cggcgtgatg gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc
     8641 tggcgaccgg cacggttgaa gcgccgcgcc gtaaccgtcg cgatacgctg ctgctgtttg
     8701 tcatttgtac attgatgtgg ggctcgaaca gcctgtacat catcaacatg ccgctattta
     8761 ttatcaacga actgcatctt cccgagaaac tggccggtgt gatgatgggg accgccgccg
     8821 ggctggaaat cccgacgatg ttgattgccg gatatttcgc caaacgtctg ggtaagcgtt
     8881 tcttaatgcg cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc
     8941 attcacctgt cattctgttg ggcttgcagc tgctaaatgc tatttttatt ggcattctgg
     9001 gcggcatcgg gatgctctat tttcaggatc tgatgcccgg tcaggcgggt tcagccacca
     9061 cgctctatac caacacgtcg cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg
     9121 tcgccgagat ctggaattat cacgctgtgt tctggtttgc gatggtgatg attatcgcca
     9181 ctctgttttg cttactgcgg attaaagatg tttaaggcgc ggtcagcatt tccagatcga
     9241 taagccaggt catggcctgc tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc
     9301 ctgcacagac tttcggtcgc agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa
     9361 gctgaatgca gggcgtattg gcgggcttgc catctggcat accgggaata gggctggaaa
     9421 ttgaaggggc agtgcaacac gccccgcaac ccggacggca ttccatacgt tgttctctta
     9481 aggttaacca ccggattgat gagcgcgcac agtaccacct tttttgcacc agcaaaagtg
     9541 cgaataccac ttgcccgaaa ggcccgtcgc gagtactttg tcgcgatatt tttgacattt
     9601 tcgactacag gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga
     9661 attacaacgg caaactgctg ctggttaagg atattgatat tcagtcgccc actgcccgcg
     9721 gtgccgctac gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag
     9781 agcgtttcaa aggtgatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact
     9841 tctcctatgt cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata
     9901 ccttcaccaa agatcagatt gaagaagagt tgctgtttat gccagagggc ggcatgccgg
     9961 acatgcaggt gctgacctgg gacggtcaac tgctggcgct tgagctgccg cagaccgttg
    10021 atctggaaat cgttgaaacc gcaccaggca tcaaaggggc atctgccagc gcccgtaaca
    10081 aaccggcgac attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg
    10141 aaaaaattcg tatccatatc gaagaacgcc gttatatggg gcgtgctgac taacttcagc
    10201 cgcatgcaga aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct
    10261 attttgcgct tcctgaacac cttacgccct caattttcac tcgttgattg atcgccctca
    10321 cacttcatcg cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt
    10381 cattgttaat ttatcgttac taagacgtga ctcctatgaa gacaattgcc tccgttacgc
    10441 tcccgcatca tgtacacgct ccacgctacg atcgccagca gttgcaatca cgtatcgttc
    10501 attttggctt tggcgccttt caccgcgctc atcaggcgtt actgaccgat cgtgtgctga
    10561 atgcccaggg cggcgactgg gggatctgtg aaatcagctt gttcagcggt gatcaactga
    10621 tgagccagct ccgcgcacag aaccatttat ataccgtgct ggagaaaggt gcggacggca
    10681 atcaggtgat aattgtcggt gccgttcacg aatgccttaa tgcaaaactg gattccttag
    10741 cggcaattat tgagaaattt tgcgagccac aggtggcaat tgtttccctg acgattaccg
    10801 aaaaaggcta ttgtattgac ccggccaccg gtgcactcga caccagtaat ccgcggatta
    10861 ttcacgatct acaaacccct gaagaacctc actccgcacc gggtattctc gtcgaagcac
    10921 tgaaacgccg ccgtgagcgc ggccttacac cgtttaccgt gctctcctgc gacaatattc
    10981 ccgacaatgg ccatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag
    11041 aactcgccgg gtggataaaa gaacacgtca gttttccggg aaccatggtc gaccgcattg
    11101 ttccggctgc aaccgacgaa tcactggcag aaatcagcca gcatctgggg gtaaatgatc
    11161 cctgcgcgat tagctgcgaa ccgtttatcc agtgggtggt ggaagataac ttcgtcgctg
    11221 ggcgtcctgc ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag
    11281 agatgaaact gcgtatgctt aatggcagcc actcttttct cgcttatctg ggttaccttt
    11341 caggattcgc ccatatcagt gattgcatgc aggatcgcgc atttcgccat gccgccagaa
    11401 cattaatgct gaatgagcaa gcgccgacac tacaaattaa agatgtcgat ttaacacaat
    11461 atgcggacaa gttaattgca cgttttgcta atccggcgct gaaacataag acctggcaaa
    11521 tcgcaatgga tggtagccag aaattaccgc aacgtatgct ggcaggtatt cgcatacatc
    11581 tggggcgcga aacggactgg tcgttgctgg cattaggcgt tgctggctgg atgcgttacg
    11641 tcagcggcgt tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa
    11701 ttcgcgaact tgttgcgggc agcagcagtg aacaacgcgt aaccgccctg ctttccctgc
    11761 gtgaagtttt cggtgatgat ctgccagata acccgcattt tgtgcaggcc atcgaacaag
    11821 cctggcaaca aatcgtacaa ttcggcgcac atcaggcgct attaaacacc ctcaaaattt
    11881 aacgatttct gcggttaaag cggatgaagc tcaccttcgt ccgctctccc cttctctttt
    11941 ctgccttttt tagccaggat taacgctcag ttaacttacc agagtattgc ggagccgttg
    12001 tgaccaggac caacctcatc accggttttc tcggcagcgg gaaaaccacg tcgattcttc
    12061 atctgttagc ccataaagat cccaacgaaa aatgggcggt actggttaat gaatttgggg
    12121 aagtcggaat tgatggtgct ttgctcgccg atagcggcgc attgctgaaa gagatccccg
    12181 gcggctgcat gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc
    12241 gtcagggaaa accagaccgc ttgttgatag agccgaccgg gctgggccat ccgaaacaga
    12301 tcctcgatct gttaaccgca ccagtctatg aaccgtggat agatctgcgc gccaccttgt
    12361 gcattctcga tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg
    12421 accagctggc tgccgcagac atcattgtcg ccaataaatc cgaccgtgcg acgcccgaaa
    12481 gtgagcaagc gctacagcgt tggtggcagc aaaatggtgg cgatcgacaa ttaattcaca
    12541 gtgagcatgg gaaagttgac ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt
    12601 tgcccgccag cgccgcgcat tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa
    12661 gcctgccaga gcatcaacgc tggcgtcgca gtctgaacag cgggcaagga tatcaggcct
    12721 gcggctggat attcgacgct gatacggtat tcgacaccat tggcattctg gaatgggcgc
    12781 gacttgcacc ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtacgaa
    12841 tcaaccgtca gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagcc
    12901 gtattgagct gatttccagc agcgaagctg actggaatgc tttgcagagc gcgctgttga
    12961 agcttcgttt agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga
    13021 aaatatgatt aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt
    13081 tctttcctgg tatattcccg ttaatcatgg attctggttg ccaattgatg cggatatttt
    13141 ttatttcttt aatcagaaac tggtcgaaag taaggccttt ttgtggctgg ttgcattgac
    13201 caacaatcgc gctttcgacg gttgttctct gctggcgatg ggaatgttga tgctgagttt
    13261 ctggctgaaa gaaaacgccc ctggcagacg acgtatcgtg attatgggtc tggtcatgct
    13321 attaactgca gtggtattaa accagctggg tcaggcatta attcctgtaa aacgggccag
    13381 cccaacattg acttttaccg atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa
    13441 agatgcctca cgagatagct ttcccggcga tcacggcatg atgctgctta ttttttcggc
    13501 attcatgtgg cgttatttcg gcaaagttgc aggccttatc gcccttatta tttttgtggt
    13561 ttttgcattt cccagagtaa tgattggcgc acactggttt actgacatca ttgtcggttc
    13621 gatgaccgtg atattgatcg gtttgccctg ggtgttgctg acgccattaa gtgatcgatt
    13681 aatcaccttt tttgacaaat cactaccggg aaaaaacaaa catttccaaa acaaataact
    13741 cacagtaatt aacatcatca ggcttatttt tatagtgagg ataatcctga tgatgcgcac
    13801 cgtgctttca tccatcgcgc gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg
    13861 tttcactcaa tcgtacttta attgaccaat cccgcttatt aactttctgt atcacttttt
    13921 cttataaaaa atcatgtaaa accgctcgcc aagaccgcac caatcgggta atctcgaact
    13981 cgttttgcct cggcggtaga ttatcctcac agcatataat tttgtgcgtt agtccacaga
    14041 tttggcctta aggaattgtt tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt
    14101 tttttataac gatatttgtc gttaaggact tcaagggaaa acaaacaaca tggtcaaatc
    14161 tcaaccgatt ttgagatata tcttgcgcgg gattcccgcg attgcagtag cggttctgct
    14221 ttctgcatgt agtgcaaata acaccgcaaa gaatatgcat cctgagacac gtgcagtggg
    14281 tagtgaaaca tcatcactgc aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt
    14341 cgacgtaaaa tcgcgaatta tggatcagta tgctgactgg aaaggcgtac gttatcgtct
    14401 gggcggcagc actaaaaaag gtatcgattg ttctggtttc gtacagcgta cattccgtga
    14461 gcaatttggc ttagaacttc cgcgttcgac ttacgaacag caggaaatgg gtaaatctgt
    14521 ttcccgcagt aatttgcgta cgggtgattt agttctgttc cgtgccggtt caacgggacg
    14581 ccatgtcggt atttatatcg gcaacaatca gtttgtccat gcttccacca gcagtggtgt
    14641 tattatttcc agcatgaatg aaccgtactg gaagaagcgt tacaacgaag cacgccgggt
    14701 tctcagccgc agctaataaa ccgtttggat gcaatccctt ggctatcctg acgagttaac
    14761 tgaaagcact gcttaggcag tgcttttttg ttttcattca tcagagaaaa tgatgtttcc
    14821 gcgtcttgat ccaggctata gtccggtcat tgttatcttt taaatgttgt cgtaatttca
    14881 ggaaattaac ggaatcatgt tcatacgcgc tcccaattct ggacgtaagc tcctgcttac
    14941 ctgcattgtt gcaggcgtga tgattgcgat actggtgagt tgccttcaat ttttagtggc
    15001 ctggcatagg cacgaagtca aatacgacac actgattacc gacgtacaaa agtatctcga
    15061 tacctatttt gccgatctga aatccactac tgaccggctc cagccgctga ccttagatac
    15121 ctgccagcaa gctaaccccg aactgaccgc ccgcgcggcg tttagcatga atgtccgtac
    15181 gtttgtgctg gtgaaagata aaaaaacatt ctgttcatct gcgaccggtg agatggacat
    15241 tccactcaat gaattgattc cggcgctcga cattaataaa aacgtcgata tggcgatctt
    15301 acccggcacg ccgatggtgc cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt
    15361 gctgaaaaat agcggcgtct ttgccgctct gaatctcaac ctgacgcctt ctctctttta
    15421 tagttcacgg caggaagatt acgatggcgt cgccctcatt attggtaata ctgcgctatc
    15481 taccttttct tcacgtttga tgaacgttaa cgaattaacc gacatgccag tccgtgaaac
    15541 taaaattgcg ggcattcctc tgaccgttcg gctttatgca gatgactgga catggaacga
    15601 tgtgtggtac gcatttttac tgggcggcat gagtggaact gtcgttggcc tgctctgcta
    15661 ttacctgatg agcgtacgta tgcgccccgg cagagaaatc atgaccgcca tcaagcgcga
    15721 acaattttac gttgcgtatc aaccggtggt ggatacacaa gctttgcgag taacgggcct
    15781 ggaagtactg ctacgctggc ggcatcctgt agcgggagaa attcccccgg atgccttcat
    15841 taactttgcc gaatcgcaaa agatgattgt gccgctgact cagcacctgt ttgagttgat
    15901 tgcccgcgat gccgcagaat tagaaaaagt actgccggta ggcgtcaaat ttggcattaa
    15961 cattgcgccg gaccatctgc atagcgaaag ctttaaagct gatatccaga aactgctcac
    16021 gtccttgccc gcacaccatt tccagattgt gctggaaatt accgagcgcg atatgctgaa
    16081 agagcaagaa gccacacaac tcttcgcctg gctgcactcg gtcggcgtag aaattgctat
    16141 tgatgacttc ggcaccgggc acagcgcgct tatctatctt gagcgtttta cgctcgatta
    16201 tctgaaaatt gaccgtggat ttatcaacgc catcggtacg gaaacgatca cttcccccgt
    16261 acttgacgcg gtgctgacgc tggcgaaacg cctcaatatg ctgacggttg ctgaaggggt
    16321 cgaaacgccg gaacaggcgc gatggctaag cgaacgcggc gttaatttca tgcaaggcta
    16381 ctggattagt cgcccgttac cgctggacga ttttgttcgc tggctaaaga aaccgtatac
    16441 gccgcagtgg taaggtgtgc ttacgtccct tattattcat agtgaaagca tgccggattg
    16501 cggctaatga tgagtaaaag gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt
    16561 tatcgctctg ttcacctttg gtgtgcaggc gcaggctatc aaggaaagct atgcctttgc
    16621 cgtgctgggc gaaccccggt acgcgtttaa tttcaaccat tttgattatg tgaaccccgc
    16681 cgcgccaaaa ggtgggcaga taacgttgtc agccctcggc accttcgata atttcaaccg
    16741 ctatgcactg cgcggcaacc cgggcgcacg caccgagcag ctgtacgaca cgctatttac
    16801 gacttccgat gacgaaccag gcagttatta cccgctgatt gctgaaagcg cacgctatgc
    16861 tgacgattat tcctgggtgg aggtcgctat taatccgcgc gcccgttttc atgatggttc
    16921 gcccattact gcccgcgatg tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc
    16981 gcaatttcgt ctggtctaca aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg
    17041 cattgagtta gctaaacccg gcaaagaaga tatgttgagt ctgttttcgc tgccggtatt
    17101 tccagaaaag tactggaagg atcacaaact tagcgacccg ctcgccacgc ctccgcttgc
    17161 cagtggtccg taccgcatta cgtcctggaa aatggggcaa aatattgtct attcccgcgt
    17221 gaaagattac tgggcagcaa acttaccggt aaaccgcgga cgctggaatt tcgacaccat
    17281 tcgctacgat tattacctcg atgataatgt cgcctttgaa gcgtttaaag caggtgcctt
    17341 tgatttgcgt atggaaaacg acgccaaaaa ctgggccacg cgttataccg gtaaaaattt
    17401 cgataaaaaa tacatcatca aagatgagca aaagaacgaa tcagcccagg atacgcgctg
    17461 gctggcgttt aatatccaac gtccagtatt cagcgatcgc cgggtgcggg aagcaatcac
    17521 tctcgccttt gactttgaat ggatgaacaa ggcgttgttt tacaatgcct ggagtcgcac
    17581 gaacagttat tttcagaata ccgaatacgc ggccagaaat taccccgacg ccgcggagct
    17641 ggtgcttctg gcaccaatga aaaaagatct accgccagaa gtcttcacac aaatctacca
    17701 gccgccggta tccaaaggcg atggctacga tcgtgacaac ctgttaaaag ccgacaaact
    17761 tctcaacgaa gcgggctggg tgctgaaggg tcagcaacgc gttaatgcca caacgggtca
    17821 gccactcagc tttgaattat tgcttcccgc aagcagcaat agtcagtggg tattgccgtt
    17881 ccagcacagc ctgcaacggc tgggtatcaa catggacatt cgcaaggtgg ataactcgca
    17941 aatcactaac cgcatgcgca gtcgcgacta tgacatgatg ccgcgcgtat ggcgggcgat
    18001 gccgtggccc agttccgatt tacagatttc ctggtcatcg gaatatatca attccactta
    18061 taatgccccc ggcgtgcaaa gcccggttat cgactcgctg atcaaccaaa ttattgccgc
    18121 gcagggaaat aaagaaaaat tactgccgtt ggggcgagca ctggatcgcg tattaacgtg
    18181 gaattattac atgctgccaa tgtggtacat ggcggaagac cgtctcgcct ggtgggataa
    18241 attctcccag ccggccgtgc gccccatcta tagcctcggt atcgatacct ggtggtatga
    18301 cgtcaataaa gcggccaaac tgccgtccgc cagcaaacag ggagagtaga tgggcgctta
    18361 cctgattcgc cgtctgttgc tggtgatccc aacattatgg gcgattatca ccatcaactt
    18421 tttcatcgtg caaattgcgc ctggcggtcc ggtcgaccag gccatcgccg ccattgagtt
    18481 tggtaatgcc ggagtattac ccggcgcagg cggtgaaggt gttcgtgcca gccatgcgca
    18541 aacgggtgtc ggcaatatca gcgacagtaa ttaccgtggc ggacgcggat tagatccaga
    18601 agtgatcgct gagatcactc atcgctacgg ttttgataag ccgatccacg aacgttactt
    18661 caaaatgctc tgggactaca tccgctttga ttttggcgat agcctgtttc gcagcgcctc
    18721 ggtgctgacg ctgattaaag acagtctgcc ggtttccatc accctcggat tgtggagcac
    18781 gctgattatc tatctggtgt cgattccgtt aggcattcgc aaagctgttt ataatgggag
    18841 ccgctttgac gtctggagta gcgcatttat catcatcggc tacgccattc cggccttttt
    18901 gtttgccatc ctgctgattg tcttcttcgc gggcggcagc tatttcgacc tgttccctct
    18961 acgcggcctg gtttccgcta actttgattc gctgccgtgg tatcagaaaa tcaccgatta
    19021 tctgtggcat atcacgctgc cggtgctggc gacagtgatt ggtggctttg cggcgctgac
    19081 catgctgaca aaaaactcat tccttgatga agtgcgcaag caatacgtgg tgaccgcgcg
    19141 tgcgaaaggg gtaagtgaaa aaaatattct ctggaaacat gtgttccgca acgccatgct
    19201 gctggtgatt gccggttttc cggcgacgtt tatcagcatg ttttttaccg gctcgctgct
    19261 gattgaggtg atgttttcac tcaatggtct gggcttactg ggctacgaag cgaccgtctc
    19321 gcgcgattat cctgtaatgt ttggtacctt gtatattttc accctgattg gcctgctgct
    19381 gaatattgtc agtgatatca gctatacgct ggttgatccg cgtattgatt ttgagggacg
    19441 ttaatgtcgc gactcagccc cgtcaatcag gcccgatggg cgcgttttcg tcataaccgt
    19501 cgcggctact ggtcgttatg gattttcctc gtcttgtttg gtttgagttt gtgttctgaa
    19561 cttatcgcca acgataaacc gttgctggtg cgttatgacg gcagttggta tttcccgtta
    19621 ttgaaaaact acagcgaaag cgattttggc ggcccgctgg caagtcaggc tgattatcag
    19681 gacccgtggc tgaaacaacg gctggaaaat aacggctggg tactgtgggc accgattcgc
    19741 tttggtgcta ccagtatcaa ctttgctacc gataagccct tcccttctcc accctcccgg
    19801 caaaactggc tgggaacgga tgccaacggc ggcgatgtgc tggcacgcat tctctatggc
    19861 acgcggatct cggttctgtt tggcctgatg ctgactctct gttccagcgt gatgggcgtg
    19921 ctggcggggg cgctacaagg ctattacggc ggtaaagtcg atctctgggg acaacgcttt
    19981 attgaagtat ggtcggggat gccgacgctg tttttgatta ttttactttc cagcgtcgta
    20041 cagcctaact tctggtggct gctggcaatt actgtcttgt ttggctggat gagtctggtc
    20101 ggcgtggtgc gggcggagtt tttacgtact cgtaatttcg actacattcg tgcggcacag
    20161 gcgcttggcg tcagcgatcg cagtatcatc ctgcgtcata tgttgcctaa tgccatggtc
    20221 gcgaccctca cctttttacc gtttatttta tgtagttcga taaccaccct gacctcgctc
    20281 gatttcctcg gcttcggtct accgctcggt tcaccgtcac tcggtgaact gctgttacaa
    20341 gggaaaaata accttcaggc cccgtggctt gggatcaccg ccttcttgtc ggtggcgata
    20401 ttattgtctt tgctgatctt tattggtgaa gccgtccgcg acgcatttga tcctaataag
    20461 gcggtgtagc atgacgcaaa ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca
    20521 tcagcaaacc gtacgtacag tagtcaatga tgtttcacta cagattgagg ctggcgaaac
    20581 gctggcgctg gtgggtgagt caggttcagg caaaagcgtt accgcgctgt caattttacg
    20641 cctgctccct tccccgccgg ttgaatatct ctccggcgat attcgttttc atggcgaatc
    20701 gctgcttcat gccagcgatc aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat
    20761 ttttcaggaa ccgatggtgt cgttaaatcc attgcatacc ctggaaaaac agctttatga
    20821 agtgctttca ctccaccgcg ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg
    20881 ccttgatcgc gttggtatcc gccaggcggc aaaacggctg acagattatc cgcatcagct
    20941 ctccggcggc gaacgccagc gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt
    21001 attaattgcc gatgaaccga ccaccgcact ggacgtctct gtccaggcgc agattttaca
    21061 gctgttgcgc gaactgcaag gcgagctgaa tatgggcatg ctgtttatta ctcataacct
    21121 cagcattgtc agaaaactgg cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga
    21181 gcaaaataac gccgctacgc tatttgcctc acccactcat ccttacacac aaaagctact
    21241 caacagtgaa ccgtcaggcg acccggtgcc gttgccagaa cctgcctcaa cgttgctgga
    21301 tgttgaacag cttcaggttg ccttccccat tcgcaaaggg attttgaagc gcattgtgga
    21361 tcataatgtg gtggtgaaaa acatcagttt tacgctacga gcgggtgaaa cactgggttt
    21421 agtgggcgag tccggttccg ggaaaagtac gacgggactg gcgctgctgc gactgattaa
    21481 ttctcagggc agcatcatct ttgacggtca gccattgcaa aatttaaatc gccgccagct
    21541 gttacctatt cgtcatcgca ttcaggtggt atttcaggat ccaaactcct cgctcaaccc
    21601 acgactcaac gttttgcaga ttattgagga aggcttacgg gttcaccagc cgacgctttc
    21661 tgccgcacaa cgcgaacaac aagtgatagc cgtgatgcat gaagtgggat tagatcctga
    21721 aacacgccac cgttatccgg cggagttctc tggtggtcag cgacaacgta ttgcgattgc
    21781 cagggcatta attcttaagc cctcgctgat catacttgat gaaccgacat catcactcga
    21841 caaaacggta caggcgcaaa tattgacgct attgaaatca ttgcaacaaa agcatcaact
    21901 ggcctatttg tttatcagcc acgatttgca cgttgtccgc gcgttatgtc atcaggttat
    21961 cgtactgcga caaggggaag tagtggaaca aggaccgtgc gcgcgcgtgt ttgccgcacc
    22021 gcagcaggag tatacgcgtc agctactggc gttgagctga cgcttaaaaa ggattgtagt
    22081 ttgaaaaggg ttcggcaatt gccacaccaa aatttttcaa tcggcatgtt gcagcaaact
    22141 cgtcctggcg atttacaaac aggcacggct caccttcaca ctccagcacc gaacatggaa
    22201 cttcgatgtc gcttaacgcc tggcttagtt tgtacatcac cgaccatgca ttatcaccat
    22261 ccgggctaag caatttaagc gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa
    22321 tcggttcaac cttcgaacct gcgaaaccag cagaccagcg aaagttctgc ggtagacgat
    22381 gaacgattga gagttgtaat gaagtcactt attttccccg gaagtacatt tacttcacaa
    22441 tttgtttaca tcaattttaa cacatcatca acaatccgtc tttatacaga tcgtaaagat
    22501 tgatgcctcg cctgcgtggc ctctatggct ctgatttaag taatatctgc gggcgtggtc
    22561 acgtttgcgg ggctatatgt acccgtttct gcgatctaac tcaacctttt taactacaat
    22621 gatgtgactt tttacataaa ttgattttac ataaaataaa catatatcgg gggaatgata
    22681 gatttgtggt tgacatgcat caacaaagga agccttttag cttcctcgtt gtgcaataga
    22741 tcaccgtttt ttcggtcgac tggcgtacag acagaagaga atggagctgg ttgcgcagaa
    22801 tgcaattgac caaatcatcg gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc
    22861 aacaattgcc cctatgccaa aacggaaggt tcctgccagc gaagatgccg ttcccgccat
    22921 atggggaaac tcatcaagaa tgaccgccat cgcattggat gacaccatcg acacgcagcc
    22981 cacaaacgcc gcaacgccaa ccaccagcga ccagaatccc agccccagca gcgcactgat
    23041 gaccatccac gccgccataa taaactgtat ccacaacccc gagcggaaca tatttaacgc
    23101 gccaatacgg cggacgaagc ggctgttaaa aatggtcatc acgaacagga aaacaatgtt
    23161 tagtgcaaag taataaccaa agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa
    23221 cggtccggcg ctcaggaacg agaacattcc ggcaaagctg aaaccgctgg ccagcatata
    23281 actcaggaca cgcttatgac ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg
    23341 aaaaggctgg cgacgctccg gtggtaaggt ttctttgatc agaaagaaga tcattgccga
    23401 agccagaatc gccgccacgg cgaggatcca gaagatgtaa tgccagctca gccacaccag
    23461 cacccagccg ccaactatcg gtgccatcag cggtgcaatg gttgtcacca gcatgacaaa
    23521 cgacatcatc cgcgagaact cttctttcgg gtagatatca cgcatcaaag cgttgataac
    23581 cacgctggcc gcagccgccg ccagcccgtg gaagaaacgc atcacaatca gctgatcgat
    23641 ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac accagcgtac cgccaagaac
    23701 caccggctta cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa
    23761 gcccagaata taagtactga gggtcatctg cgtactgccc gccggtacgc caaactgcgc
    23821 tgaaattacc ggtagcgcgg gcagatacat atcaatcgac agcggcatca acatggccag
    23881 caggccaagg ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc
    23941 ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata
    24001 ttcaccgggg gctaaatcag catccagcgt aatgccgcca atacgttcac gatgcagctc
    24061 aaccacatgg ttacccacgg cggcgaacat gcgtttcacc tgatgataac gcccttcgct
    24121 gatggtcaga cgaacctgcg ttggggtaat cacttccagc accgccggct tagtgagatc
    24181 tttttcgtta tgcagctgca cgcctttagc aaattgctct gccgtatcgt cagctacggg
    24241 tgattccagc gtcaccagat aggtcttctc gcaatgatgg cgcggagaag taatgcggtg
    24301 cgaccactga ccatcatctg tcatcagcac cagaccggtg gtatcgatat ccagacgccc
    24361 tgcggcgtgc agtttccagg ctaccggttc atcaagaaaa tagagcaccg ttgggtgatc
    24421 agggtcgtcc gtggagcaaa cgtagccctg aggcttattg agcatgaagt aacgtggacc
    24481 gtgttgctgc gccagcgggt tgccatcgta agcgacatca tgttcaggaa gcagtttgaa
    24541 cgctgcatta cggacgattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc
    24601 ggcaatagca cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt
    24661 gattttgcct gtaaaaaacg gaggtcgggt aatgacccga aaatctgaac tgttgtctgc
    24721 ccagtatagc ggtctaataa catccctcaa gggaaaaaga tccatggcat actattagca
    24781 gaataatcta cctacgcgag accatgattt ttacacttcg cccatatcag caagaagccg
    24841 tggatgccac gctcaaccat tttcgtcgtc ataaaacccc tgccgttatc gtgctgccta
    24901 ctggtgcagg taaaagcctg gtgatagcgg aactggcgcg gctggcgcgt ggtcgcgtgc
    24961 tggtgctggc acacgttaaa gaactggtgg cgcaaaacca tgcaaaatat caggcactgg
    25021 gactggaagc cgatattttt gccgccgggc taaagcgcaa agagagccac ggtaaagtgg
    25081 tatttggcag cgtgcagtcg gtcgcccgta atcttgatgc ctttcagggt gaattttcgc
    25141 tgttgattgt cgatgaatgt caccgtattg gtgatgatga agagagccag tatcagcaaa
    25201 tcctcactca cctgacaaaa gtgaatcccc acttacgcct gctggggctg actgccacgc
    25261 cttttcgatt gggcaaaggc tggatctacc agttccatta tcacggcatg gtacgcggcg
    25321 atgagaaagc ccttttccgt gactgcattt atgagctgcc gctgcgttat atgattaaac
    25381 acggctatct gacgccgcca gaacgactgg atatgccagt agtacaatac gatttcagcc
    25441 gcttgcaggc acagagtaac gggctgttca gcgaagctga tctcaaccgt gagctgaaaa
    25501 aacagcaacg cattacgcca catatcatca gccagatcat ggagttcgcc gaaaagcgca
    25561 aaggggtaat gattttcgcc gcgacggtcg aacacgcaaa agagattgtg ggattactgc
    25621 ccgccgaaga tgctgcactg attactggcg acacccccgg cgctgagcgc gatgtgttaa
    25681 ttgaagattt taaagcccag cgttttcgct atctggtcaa cgtcgcagta ctgaccaccg
    25741 gatttgacgc cccgcacgtc gatcttatcg ccattctgcg ccctaccgaa tcagtgagtc
    25801 tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc tggcaagact gattgcttaa
    25861 ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc ggaagttggt acaccgaaag
    25921 gcaaaagtga caacgtcccg gtacaggttt tctgccctgc ctgcggtttt gccaacacct
    25981 tttgggggaa aacgaccgcc gacgggacat tgattgaaca ctttggtcgt cgctgtcagg
    26041 gatggtttga agatgacgac ggtcatcgcg aacagtgtga cttccgtttc cgttttaaaa
    26101 attgcccgca atgtaacgca gaaaatgata ttgccgcccg ccgctgccgc gaatgtgaca
    26161 ccgtactggt tgatccggac gatatgttaa aagcggcgct acgactgaaa gacgcgctgg
    26221 tattacgctg tagcggcatg tctttgcaac atgggcacga cgagaaaggc gaatggttga
    26281 aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga gcgtttccgt ctgcaaacgc
    26341 ccgcccagcg aactgccttc gagcagcttt ttatccgccc gcatacgcgc acaccgggca
    26401 tccctctgcg ctggatcacc gccgccgata tcctcgccca gcaagcgtta ctgcgacacc
    26461 cggattttgt cgtcgcccgc atgaaaggtc agtactggca agtgcgtgaa aaagtgttcg
    26521 attacgaagg tcgttttcgt cgggcgcacg aattacgcgg ttaatggtag ttttgattga
    26581 tgtataaggc gattgagtat agaatctcgc ccgcttttgc atacgcaaag cagatcactt
    26641 acctgttgct gggtcgcctg tagcaggatt aatttaagag agaaagaaat gtttactatc
    26701 aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc gccgcctgcg tgccgctaac
    26761 aagttcccgg caatcatcta cggtggcaaa gaagcgccgc tggctatcga gctggatcac
    26821 gacaaagtca tgaacatgca agctaaagct gaattctaca gcgaagttct gaccatcgtt
    26881 gttgacggta aagaaatcaa agttaaagct caggacgtac agcgtcaccc gtacaaaccg
    26941 aagctgcagc acatcgactt cgttcgcgct taattgctga ataagttgta aaaaaccccg
    27001 ctccggcggg gttttttgta tctgcagatt atgcctgatt acggtattgc tattttttgc
    27061 aggccagata aggcgttcac gccgcatctg gcattcagaa cacaaaaccg attaattgcc
    27121 gccagacgtc cggcgctgta attggtcgcg caaattcggc ggcgtgcctt taatggtcaa
    27181 ggtatcggtt gctggatccc agaagatgcg ttcacctaac agcatcgcat caaagttaat
    27241 agtcaagcca ccgccgctac cggcaaactt cgtcaactga cgcagtgtgc tacggtctgc
    27301 cgggaagctc tcttccagtt cgtagccttt ttcagccgca aactccgtga aactcacttc
    27361 gctgacgcct gccagctctt tcgacagcga ctccagttca atctcttcgc ctgcttgcag
    27421 ctgctcgtta cagtagctat aaacctgctg gcgcacattc tgccgctcgg ctttatccag
    27481 ttgcgcttgt gcagtgaaat catccaccgc ttgcagcaag ccacggtttt gcgcctttgc
    27541 attcagccct tcgctggcac caaggaaatc cataaagaaa tcggcgacct tgcgccctac
    27601 ccgccctttc aggaaagtta aatagcgggt cgattccggg ttggtttccc attcggttaa
    27661 atcgatgcgc gcgacgatat ccgcatggtt gatatcgaga tagtgcgttg ggttgatgtc
    27721 gagattttcg ttaacgcgca tactgctcag gttgctcagg actgccacca gcagatactc
    27781 caccgccaga taacggtagt ggcaaaacag cacgaatcca ccgtcagcaa aaggatattt
    27841 cgctaattcg tcacgcaggc gaccagttgc cgcgcggcta aatgccagaa agtcctcttc
    27901 gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc tcttcgctaa acaagccgta
    27961 agctttattt ttggcgctat agacccgatg cagttctgcc accatctcaa cgacggtttc
    28021 tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg ttctgctcat cacgcttgat
    28081 aagctggtgc agagcaatct ggttgatatc cagactcatg ataaactctc ctttaagacc
    28141 gggcggtatt caaccaccgc cggttaagcg acgcaataaa agatggcggt cattttacag
    28201 aaggcagcgt aacgagaatg atttaacgca taaaaaagca gaaaaaaaac cgttgctacg
    28261 gtaatatgtt gccctttcat caactaactg atttcgattt atgccacaaa tttcccgcta
    28321 cagtgatgaa caggttgaac aattgctcgc agagctgctc aacgtactgg aaaaacataa
    28381 ggctccgacc gacctttccc tgatggtgtt aggcaacatg gtcactaacc ttatcaacac
    28441 cagcattgcc ccggcccaac gccaggcaat tgccaactct tttgcccgcg ccttacagtc
    28501 ctctatcaac gaagacaaag cgcactaagg gaaacagata acaggttatg gtaactcatc
    28561 gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg ggggcactgg tttgcactgt
    28621 tcaatattct gctttcgctc gtcattggca gccgttacct gtttatcgcc gactggccga
    28681 ccacgcttgc tggtcgcatt tattcctacg taagcattat cggccatttc agcttcctgg
    28741 tgttcgccac ctacttgctg atcctcttcc cgctgacctt tatcgtcggc tcccagaggc
    28801 tgatgaggtt tttgtccgtc attctggcaa cggcgggaat gacgctatta ctgatcgata
    28861 gcgaagtctt tactcgtttc catctccatc ttaatcccat cgtctggcag ttggttatca
    28921 acccagacga aaatgagatg gcgcgcgact ggcagctgat gttcatcagc gtgccggtta
    28981 ttttattgct tgaactggtg tttgcgacgt ggagctggca aaagctgcgc agcctgacgc
    29041 gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt tatcgccttt atcgcctcgc
    29101 atgtggtgta tatctgggcc gatgccaact tctatcgccc gatcaccatg cagcgcgcta
    29161 acctgccgct ttcgtacccg atgacggcgc gacgttttct tgagaagcat ggcctgcttg
    29221 atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa tccagacgcc gtttccgttc
    29281 agtatccgtt aagcgaactg cgctatcgcg atatgggcac cgggcagaat gtcttgttga
    29341 ttactgtcga tggcctgaac tactcacgct tcgagaagca gatgcctgcg ctggcaggtt
    29401 ttgctgagca aaatatttcg ttcacgcgcc atatgagctc cggcaacact acagacaacg
    29461 gcatctttgg cctgttctat ggcatctcgc cgagctatat ggacggcatt ctgtcgaccc
    29521 gtacgcctgc ggcattaatt actgcgctta atcagcaagg ctatcagctg gggttattct
    29581 catcagatgg ctttaccagc ccgctgtatc gccaggcatt gttgtcagat ttctcgatgc
    29641 cgagcgtacg cacccaatcc gacgagcaga ccgccacgca gtggatcaac tggctgggac
    29701 gctacgcaca agaagataac cgctggttct cgtgggtttc tttcaatggt actaacattg
    29761 acgacagcaa tcagcaggca tttgcacgga aatatagccg ggcggcaggc aatgtcgatg
    29821 accagatcaa ccgcgtgctc aatgcactgc gtgattctgg caaactggac aatacggtgg
    29881 tgattatcac tgccggtcgg ggtattccac tgagcgaaga ggaagaaacc tttgactggt
    29941 cccacggtca tctgcaggtg ccattagtga ttcactggcc aggcacgccg gcgcagcgta
    30001 ttaatgctct gactgatcat accgatctga tgacgacgct gatgcaacgc ctgctacatg
    30061 tcagcacacc tgccagcgaa tattcgcaag gtcaggattt gttcaaccct caacgccgtc
    30121 attactgggt taccgcagcg gataacgata cgctggcaat taccaccccg aaaaagacgc
    30181 tggtgctgaa caataacggt aaataccgca cttacaactt acgtggtgaa agagtgaaag
    30241 atgaaaaacc acagttaagt ttgttattgc aagtgctgac agacgagaag cgttttatcg
    30301 ctaactgatt aattataaat cagttagcga aatatcttac ttgcaatcgg tgtggaaaac
    30361 ggtagtatta gcagccacga gtcggcacgt agcgcagcct ggtagcgcac cgtcatgggg
    30421 tgtcgggggt cggaggttca aatcctctcg tgccgaccaa aaatcccaaa aaaaaccaac
    30481 cattacggtt ggttttttta tatctgcaat taattcgata aacagaccgt gacacatcac
    30541 agcctgttta ttttctgtta tcagaacgtc cagaccacac ccgcctgagt attccacggc
    30601 gattctaccc ctgcgccatt gccatagctg actgacaaat gaccgcttaa cgtcggggta
    30661 aacgatgacc ttatcccagc ctgataaacg ccacgggctc cagccacatc attattaaat
    30721 ttgccatcgt cattaacttt cacctggtta gaatcggcgt attcctgacg tacggcggct
    30781 ttcagccagg gttccagcgt cgtaccattt tgcaggtcca tgtgatagct taccgccgtt
    30841 cccgcttcag cgcgtaatat ccgggtattt cccacatcag cgcgcatgcc gtttgataac
    30901 gtgtagtcct gaccatctgt ggtaaagccg gtaaaggcca gatagggtct aacactccac
    30961 aatccgtcaa cccaacggaa cccgctctca acatgagcac ccgcgccgtt actattgtaa
    31021 tcgccaaacg ctgttgcccc attactcatc ttgccatgga tggtgttggc aaaacggtca
    31081 actttcacca ccccatcaac ataggcaccg ttctgatgct cccaaccggc ataagccccc
    31141 agggtatagc tatcgatatt accttttccg ccgcgatcaa aaccaatatc agaatgagag
    31201 taaccaaaga acaagccgcg aattgtactg ctttcttcac gggagaaacg gctatcgata
    31261 ccgagcgtca ggcccgtcaa tgtttgctca aaaccagctc ccgcatcagt ggtcacgttg
    31321 ttgcgggtgt taattgccga actccacatc gccgtatcgt aactaacgcc ttttacgcta
    31381 ccaagacgct cacgcacggt gtccagttct gcatcaaata ccagcggttg tgcggccgcc
    31441 atattcagca catcagtggt tgaaggggta atttgcgcgc gattctctgc caaactccag
    31501 ctatggttgc cattatccag caaggtatat tcatacgtac cgatatcaac aacgcctcca
    31561 gcattgccca acgtaaatgc agcatcaccg ccgcccgttg ttaccagtgt aaggctatct
    31621 cctgctgccg ggctggcacc ggtgtccgtc acgaatattt tgaaatcacc tgttgcctga
    31681 ccggtgacgt tgagctgatc gctctgatga ttagccatat cggtacgcat ataaaaattg
    31741 ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat tttccgcact tgccgtcact
    31801 gatgaacgag tcactggctc atccatcata atcacatttg cgtcagacat cgcaaggttg
    31861 gctaagttga ggagggtgtt ttggttcgtc gtagtgatat cggcaatgat ggtccatacg
    31921 ctattttcga gcgaaacgtc cgctttgctg gtatccacgg caccatgcgt tctaaaagaa
    31981 gcgccctcag aaaccactac cttacccacc aacattgcgg gtgcataatt tatttgcggc
    32041 ttcatgacct caagaatgcc atcattccct ctgactgaaa cgttaaccat tgtgccagcc
    32101 cagacgttag cgcggccatt gttgataacc atattttcag agatggcttt tgaggaatac
    32161 gtgatacttc cattctgatc tgatcgaccg agatcataca ccgcatttgc ctgcactctc
    32221 gtcccagtat cctttcccag cgattgcata gtggcatgct catcaaggat agtatcaatg
    32281 gcctgcgtgt cctccataac aatgagcccg gaaccatcat ccagttcata attttttgac
    32341 acaccatctt ttatactaaa ttgaccttta ctgtttgtac cactcacttc cagcgcattc
    32401 gttgagacaa ttaattttcc gcctgctttc tgatctaccg caatcgctaa tcccccctct
    32461 ttaacctcca gtaatcctcc cttttcgata gcggcaagga cggtaatccc atctttttca
    32521 acgattaagc gcccgccttt aatcgtggga tcattcgctg cgccataaac ctgtagcaca
    32581 ccattattga gggtagtctg gtttgccgcg ccacccattt gaacaatttg ataaccgtca
    32641 tttaatactg agtattctgc ggtgccattc atttcaatgt actgataccc gccgttaatt
    32701 tcagtattac tacttatacc aaattcttta atatgctgtt ctccgccagg attgattttc
    32761 gtattattgg cggtcccata aaccagctgg atatcttttt ccaggacaac accatcaact
    32821 gtctcaccat actcaactgt tgatgccaat gctatgggtg aatataacgc tgtagccacc
    32881 agaacagata ttgcggaacg acaaagagaa acagaaccag attgatgcat tgagctttca
    32941 tcctatgaaa ttaattgcag ttaaaagcat tgggtacaga aaatacccat agctccatac
    33001 ccggagtcag tttttaaaaa ctgtttaaag aaatgcacaa gtattgtgat tgatttttta
    33061 gttgtttttc ttgatgagaa gctgatgcaa aattccgtct ttataatgaa aatgatgcca
    33121 aagcgaacga caaggttgta gttttcacta catgtccata cataaaatgg ggtaacattc
    33181 acgcgcctgg tagcgttacc aacgctacgc tcaaacataa tgattctaat aaaacctcag
    33241 gagactacta tgcctgaagc aacgcctttt caggtgatga ttgtggatga tcatccactt
    33301 atgcgacgcg gtgttcgtca gttactggag cttgatcctg gctttgaagt ggtcgccgaa
    33361 gcgggcgacg gcgcgagcgc gatcgatctg gcgaatagac tggatatcga cgtgatcttg
    33421 ctggatctca atatgaaagg tatgagtggc ctggataccc tcaatgcctt gcgcagggat
    33481 ggcgttaccg cgcaaattat tatcctgacc gtatccgatg cctccagcga tgtctttgcg
    33541 ctgatagacg caggcgcaga cggttatctg ttgaaagaca gcgacccgga agtattgctg
    33601 gaagcgattc gtgccggagc gaaaggcagc aaagtcttta gcgaacgcgt caatcagtac
    33661 ttacgtgaac gtgaaatgtt tggcgcggaa gaagatccct tcagcgtgct gacggagcgc
    33721 gagctggatg ttctgcacga gctggcacag gggctgtcaa ataaacagat tgcctcggtg
    33781 ttgaatattt ccgagcagac agtaaaagta catattcgca atctgctgcg taaactcaat
    33841 gtccgctcac gcgtggcggc caccattctg ttcctgcaac aacgcggggc acaataaaaa
    33901 tagcccgatg gcatcaccat cgggcctctt tttatttact ctcctgcggc gacaaatgtt
    33961 gcatcgcctg cgcgatacta cgttcaatca ccgcacggcg agtatcgttg gcaggtaaga
    34021 gtttcaacat catctcccac gcggcaacgg cttcgccaaa tcgctgctgc tcaaaggcgt
    34081 taaacgcata catgcttaac acacgaatat tgctatggtc cgttctcacc aactggcgca
    34141 gcagttcgcc gccgagacgg ttgtcgttgg gatcagatga acgtgtcaac gcttcagcgt
    34201 atcccagtgc agcatcgctg ttcttcggat cgaggcgata cgcagtggcg tatgcatcgg
    34261 tggcgatact ggcgttaccc agcgccatgc caacgcggcc caacataatc cagccttcta
    34321 tatcacccgg atttttttgc agttgagtac gcatccccag cgcaaggcgc gacatctctt
    34381 cttcgttgag cggatcggct ttcggatcca gcgccctgtc cagcaacgcc ggagcctgtg
    34441 ccgtggcctg ctgccagatt ttcacctgct gataattgcc agtctggtag tagctgacgc
    34501 cagccacaat tagcgccacc acaatacccg gcagataaac aacatatccg gcacgcttac
    34561 cttccggcac gctttgttca ggaaacgctt ccggcaccac gcgtacccga cgccgcgaac
    34621 gggcgtaaat gacccaaccg ccaatgccaa tagccactac cggaagcacc cacagcagca
    34681 cggtcagcgg cgttaacggc ggatcgtaag tgacgaagtt gccgtaacgc gccaccatat
    34741 aatcgacaat ctctttttta cttttacctt cctgcatcag ttcatacact ttctggcgca
    34801 ggtcggtggc aatcatcgag ttggaatcgg caatgctgtt gttctggcat ttcgggcagc
    34861 gcagttcttc agtgagctga cggaactgtt gttcctgtgc ttcatcttta aactgcaaca
    34921 cgtcgatggt cgccagcgct gagccggaga tcatcagcat cagcacgccc aataaaaacc
    34981 tcattgtgcg gcctccttac tgtatttctc ccacagcggc ttgatctctt cttcccagac
    35041 gcgaggattc agatcgcccg catggcgata gcgaatgatg ccgttgccgt caataaggaa
    35101 cgtttctggc gcgccataga caccgagatc cagccctaac atgccgtcgc catcaaacag
    35161 gcttagcgcg taaggattgc ccagctcttt cagccagctg attgccttct ggcgatcgtc
    35221 tttatagttc atgccgacca cgcgaatgcc ctgcgctgaa agctgattca gatattgatg
    35281 ttccgcacgg caggtcggac accaggtcgc ccagacgtta agcagtactg gtttgccctg
    35341 agtcagcaca tccgcctgat aaaactgccc cggattgtcc agtgattcga gacgaaactt
    35401 cggcacaggc ttgccaatga gcgccgattc cagattggtc ggatcatccc cttcggcatt
    35461 acgcgccagc tgccacagca gcgccgcggc aatcgccagg aagataatca acggaattaa
    35521 caatactttg cgcttcatac ggcctccggc gcagtttttt gcggactcac gcgcttacga
    35581 tagcgaggat caaacagaca cagtagtccg cccaacgcca tcatcagccc gcccgcccaa
    35641 atccagcgaa caaatggttt gtagtaaaga cgcacggccc acgcgccgtt ttccagctct
    35701 tcaccgagcg cggcgtacag gtcacgcgtg atgccgccgt caattgccgc ttcggtcatc
    35761 atcgacccgg cagtgttgta ataacgtttt tccgcataca gcaccgtttc cggtttaccc
    35821 tctcgcgtta cgccaatagt cgccacaccg ccgcgccagt tcgggccagt cacctctttg
    35881 acatcacgga aggtgaagcg atattcatga atatcgacgc tatcgccgga cttcatgcgc
    35941 acatcacgct caacgctata attctggcta aaggcaatgc caacaattgt cactgccagc
    36001 cccaggtgag ccgccaccat cccccaataa ctgaaggtgg ttttcgtgcc gcgtgaaata
    36061 cgtagcgcag cttccgcaat tgccagcacc gcaatccagc aggccattgc caggccgagc
    36121 accgtcatcg ccaccacttt gctttcgaac agccacggca acagcagaga cagcaccagc
    36181 gtagagatga aggcgataat caataaattg cggattttac gtgggcgatc gcgcccccag
    36241 cgcaccagag gaccgacacc cagcagtagc gcaaacggca ccatcagcca ggtaaacatg
    36301 gtgttgaaga acggttcgcc aatcgaaata ctgcccagtc ccagttgctt gtgcaccagc
    36361 ggcagcagcg tccccagcaa caccaccagc atcgcggcga ccagcaaaac gttgttcgct
    36421 aacagcagag attcccgcga ccacagcgca ttgtttacgc gtgagcgaac tttgtgtcca
    36481 cgcgcggcaa acagcagcag cgaaccgcca atcaccagca ccataaaggc gaggataaac
    36541 ataccgcgcg ccggatcaga cgcaaacgcg tgtaccgata ccagcacgcc ggaacgcacg
    36601 aggaaagtcc ccaacagaca caacgagaag gcactgattg ccagcaataa tgtccacgct
    36661 ttgaagctgg cgcgttgttc agtgaccgcc agtgagtgca tcagcgcagt ccccaccagc
    36721 cacggcataa acgaggcgtt ttctaccgga tcccagaacc accagccacc ccagccgagt
    36781 tcgtaatagg cccatgcgga accgagcaca atgccgagcg tcaggaaaat ccacgccgcc
    36841 agcgtccacg ggcgagtaaa acgcgcataa gtgctgtcca gacgcccgct cagcaaagaa
    36901 gcaatggcaa aagcaaacgc caccgagaaa cccacgtacc ccatatagag cagaggcgga
    36961 tggaagatca gccccggatc ctgtaacagc gggttaagat cgcgcccttc aatcgggaag
    37021 ttcggcagcg tgcgtgagaa tgggttagag gtaaagagaa tgaacagcaa aaagccgacg
    37081 ctgaccatcc ccattatcgc cagtacgcgg gccacaatat ccagcggaat acgctgacta
    37141 aaaatcgcca ccgcaaaggt ccagccgctc atcagcagca cccacagcag cagtgagcct
    37201 tcatgtgcgc cccaggtcgc cgccacgcga taccacaccg gaagctgggt attggagttg
    37261 ctggcaacat aggtgacggt aaagtcgttg accacgaaag cattgaccag caccagaaat
    37321 gcgccagcca cagacataaa cagcagccag gcaaacaagc gggaagacgc catcatgcgc
    37381 gcatctccgc gcgccacgcc ccatagcgga tacacggaca gcagcagcgc aatccctaac
    37441 gccaggcaca gcagcccgtt tccaatttcc ggcatcatga tgctgggtcc ttataaacac
    37501 tcgccgggcg acggtggtta gcttccatcg ctttctcaac ttctggcggc gtgtagtttt
    37561 cgtcgtgttt cgccagcact tcttttgcga ggatatgatt gcctttttcc agctcgccct
    37621 gcaccacaac gccctgccct tcacggaaca gatccggcaa aatgccttcg taagagacat
    37681 ccactgagcc ttcagcgtcg taaatggtga aggtcacttt cagcgaattg ggatcgcgct
    37741 gcacactacc cggcatcacc atcccgccaa cgcgcagacg ctgaccgact tccggcattt
    37801 gctgagtttc acgcttgccg tagagaattt cccccggcgt ataaaagaga tcgatattcg
    37861 agcgcagcgc atatagcacc agaccgatag tcagcgccag ccctgccaac acggcacagg
    37921 caatccacaa gcggttttta cggcgaatat tcatgcagcc tcctgctgtt gcgcagcacg
    37981 taaacgcgcc tcacgcgccc gctgttgcgc cacgccacgc agaattgcgc gatgttgcat
    38041 caccgagtgc acgaccaaaa ccaccagcgg aataacggtc atcaccaccg ccagccagac
    38101 aaaaaaggcg taaccgccca ttgcgaaaaa ttcattccag gaagcaaatg caggggtcat
    38161 ttacggcctc ttttcagtat cagttcactc acccacggac ggcgtttttc catcagcaaa
    38221 atcaaattac gcatccgcat cagcgtcagc gtggcagaca gtagcaggaa gccaaaaatc
    38281 gaccagcgca gcggcgaacg catcgccgga tcgatacttt gctgcatccg cgttgatccc
    38341 tgatgcaggg tgttccacca ctccacggag taatgaataa tcggcagatt caccacgcca
    38401 atcagcacca gaatacctgc cgcacggccc gccagacggc ggtcgtcgaa ggcgtgccac
    38461 agggcaatca cacccacata caaaaacagc agcaccagtt cagaagtcag acgtgcatcc
    38521 catacccacc aggtgcccca catcggtttt ccccatgcag agccggtaac cagggcaata
    38581 aaggtaaaca cggcaccaat gggggccatc gccgccaccg ccaggttggc cattttcatc
    38641 tgccagacaa ggccaataaa cgccgccacc gccattgatg cataaatgcc catcgaccag
    38701 atcgccgcag gcacatgcag gtagataatg cggtagctat ttccctgctg ataatcagcc
    38761 ggagcaaagc cgaatcccca gatccagccg acggtaagca cgaccacact ggcaattgcc
    38821 agccacggta taaaccagcc acagatttga tacagccgtg gtgggatcgc cagttgatgc
    38881 agtgttttcc acatagtttc gataccagac tcgaacaaaa atcagtaatc cagcgttatt
    38941 gaatgctgat tcgtaacgct gccgccgtcg caaaaggact taatgtcgcg gtgcctgcca
    39001 gcaacgcgcc taaaattgcc agatacccgt caacgggcaa atgcatggaa gccgcgtcca
    39061 tcgcggcggt ggcaaagatg agtaatggga tagtcagcgg taacaccagt atgctgagca
    39121 gcacaccacc gcgcttaagt cccactgtca acgccacacc cggtgcaccg agaaagccaa
    39181 gcgtaggcgt tcccagcagc agcgtcagcg ccatcacttg ccagccataa acatccattc
    39241 ccagtagcat tgctaccagt ggcgaaagga tgagtaacgg cagaccggtt accatccagt
    39301 gcgccatcac cttcgccagc acaacggcgg gcaagggtaa cggcaacaac atcaattgtt
    39361 caagactgcc gtcctgcaaa tcgtcacgga acagtcgttc cagcgccagc aaggatgaaa
    39421 gcagcgcagc aacccagata atgcccggtg caatacgcgc cagcagttgc ggctccggac
    39481 cgatactgag cggaaaaagg gtaattacaa tcaggaagaa ccacagcggg ttggcgattt
    39541 cggcgctatg gcgaaacgct acacgcagct caagacggaa aatgcgccag aacatcatgc
    39601 ggccctcgtt tgcgtcagtg aaatgcggcg aattttactt tcagcaacgt tgagcggctg
    39661 gtgggtagtc agaatcacaa tccccccctg ctccgtatgc tgcgccatac gctgggtcag
    39721 acgatcgacg ccgttaacgt caatcgcggt aaaaggctcg tcgaggatcc ataacgtggc
    39781 acgggtcagc cacagacgcg ctaaagcgac gcggcgttgt tgcccggccg agagctgatt
    39841 tacaggaata tcttcgaatc cggcaagccc ggcctgcgcc agggcttcca gacattgtgc
    39901 ggtatcgcca tcgcgatgat aaaagtgcag attttctaac gccgtcagcc gggttttgat
    39961 ccccggctga tggcctatcc ataacaggtt ttgatggtag ctgtcgcgta cctgatgcaa
    40021 gggctgccct tgccagagaa cctcgcctgc gtcagggcga gacaaccccg tcagcaaacg
    40081 gagaagcgtt gtcttccccg cgccgttgct accggtgatt tgtacccact ctcctgcgtt
    40141 cagcgtaaat gacaagccac taaataaggt tcgttcatcc cgctcacaaa gtaactctct
    40201 ggcttcaagc atacccacgc aataaccctg ttaaaaacct ggctcgactt cacgcatatc
    40261 gggcagcttg tgcgctatcc ctttatggca atcaatacag gtttgcccat ctttcaccgc
    40321 ctggtcatgc atcttcgcgg caaccgattt ctgggcggtt gtatccatat actcgaagtt
    40381 gtgacagtta cggcactcct gcgagttatt gtccttcatg cgccgccact cattctgtgc
    40441 catcgtcaga cgatgagctt caaatttctg cggcgtgtca ataacgccaa aaattttacc
    40501 atacagctct ttacttgctt tgagcttgcg tatcatcttc ggcacaaact cgtgcggaac
    40561 gtgacaatcc ggacaggtcg cacggacgcc gctacggttg ttgtagtgca cggaatccat
    40621 gtattcctga tacaccgtgt tgcgcatttc gtggcagcta atgcagaact cttcggtatt
    40681 ggctttttcc atcccggtgt taaagccacc ccagaagacg atgccgccaa caaaaccgat
    40741 caacagcagc gtccccagcg ccagacggct gggggtacgc caccatttcc acaggcgctt
    40801 aatcagacca ggcttacggt cagaatttcc cataataacc tcttatttcc cgtaaccttt
    40861 tgatggggta aaggtattcc ccacgattgg cgcggtatcg gcctgcggta cgtgacattg
    40921 cagacagaaa taacgacgcg gagccacttc cgcgcccact ttgccgtcgc tgtccataaa
    40981 gtgagtagga ctgatacgcg gcgcgccagt ggtgcgatag ctttcgacac cgtggcactg
    41041 caagcagcgg ttggtattgg tcgttacctg ataaccttca acgctatgcg ggatcattgg
    41101 cggctgattc acatagttca gcggcatccg ttcctgctct tttggcatcc gaatggcccc
    41161 ttcctgtgtc ccggatactt ccggcgactg gctaaaatcg acgccattag ccgcccaaac
    41221 cgcgccgctt accaccaagg ccagcatcgc cgtccattga cacagcgctt tcttcaggtc
    41281 atggcttttc atgatttcgc tcccgaactc catcgtgtag ttattgtaaa tacatcctca
    41341 gaacagacat ccacgcagcg accgcacgtc atgcaatcgc ggctggtgac ctgcaccggg
    41401 ctttgttcat ccagcaccgg ggcacgtagc acatgcggtt ccgggcaaac atgaaaacaa
    41461 tccatacagc ggttacattt ctgccgatcg gtggccgcaa cggtaatcac acctttgcta
    41521 cccagcacgc catacagcgc gccaaccgga caaatgtgcc cgcaccagcc gtgttcaacg
    41581 accagtagat caaataaaaa cagcgcgaga ataagcagcg cgccgctgcc gaagcccatt
    41641 accaggctac gccccatcag agaaacgggg ttgatccatt cccaaatgag cgtgccagtt
    41701 aaggctgatc ccaccagaat gaccaccaac agcacgtagc gaatgtggcg agggatcgtc
    41761 gcagactgat tgaggtcaaa gcgcctgcgt aaccagttcg ctaagtcggt aatcgggttc
    41821 agcgggcaga cccagctgca aaataatcgc tttcccgcca gggcgtagag cacggtgata
    41881 atcaccgcgc cggtcaacgc caccgtggca ggcagatgac cactggcaag actttgcagc
    41941 gtcatcagcg gatcggtcag cggaacggtg tcgaataaca ggctactgct gtagttgccg
    42001 tgcaagatcc acacaccaaa ccatggaccg ctcaaaaaca tccccagcac gaagaactga
    42061 caaaggcgac gtaacaccag ccaacggtga ctgcgccacc agcctttttt ctccagcgcc
    42121 tcgcgcccgg cgtcacgttt acgatttgcc attgttcccc tccagccagc cgaagcggta
    42181 atggtgacct aactcccctt tcgccagtga cagcggtaac accttgattg ccggttgttc
    42241 cagcacgcat actttttcgc acttaccgca accggtacag gcgtcgctgt gaaccgtcgg
    42301 cagaaagcgg gcgtgcttac cggtacgcgt gttgcgctcc agctccaggg tgatggcctc
    42361 atcaattttc gggcattcgc gataacaaac atcgcagcgc agcccctgaa agttgagaca
    42421 gttttcctgg tccaccagta ccgccagccc catccgcgcg tcgtcgatcg attcaatctc
    42481 acgatccagc gcaccgcttg ggcacacttt ggcgcacgga atgtcctcac acatttcgca
    42541 aggaatatcc cgtgcgacaa aatatggcgt gcccgccgac agaccagagg ccagcgtcgc
    42601 cagttttaag gtgtcgtaag ggcaagcctg aacacactga ccgcaacgca cacaggcact
    42661 ggcaaaggcg ttctcgttta tggctccggg cgggcgcaac cgcacgccag atgcgcgtgc
    42721 ggtttgctgt tgtaacccca gcgccacacc cacggcagcc agcccgcctg ctgtgcgaac
    42781 aacatcgcgc agaaagcggc ggcgaccatt ttgaggtttc gctgaccggg acataatggc
    42841 ttacaccttc tccagtttga ccgcgcactt cttgaagtcc gtctctttcg agagcggatc
    42901 ggtcgcatcc agcgtcagtt tgttaaccag ctgtgcggcg tcgaagaacg gcatgtacac
    42961 caggccctgt ggcggacggt tacgaccgcg cgtttcaaca atcgagatca cttcgccacg
    43021 gcgagaaacc actttcactt tgtcgccacg gcgcagatcg cgcgctttcg catccagcgg
    43081 gtgaataaac aagaccgctt ccgggaaggc gcggtgcagt tccggcacac ggcgagtcat
    43141 actgccggtg tgccagtgct ccaggacgcg tccggtagag agccacaggt cgtactcttc
    43201 atccggtgct tccgccgccg gttcgaacgg cagcgcgaag atcaccgctt tgccatccgg
    43261 tttaccgtag aacttgtagc cttcgcccgc tttcacgtac gggtcgttac cttcgctgta
    43321 acgccactgc gtttctttac cgttcaccac cggccagcgc agaccgcgcg ctttgtggta
    43381 gtcatcgaac ggtgccagat cgtgaccgtg accgcgacca aaccatgcgt actcttcgaa
    43441 cagccctttt tgcagataga agcccagctc gcgggattca tcgttcagct gatcttccgc
    43501 cagttcggat accgggaatt tgctcacttc gggggtggca tacagaactt cgtacagcgt
    43561 tttgccacgc agttccggtt tcttcgccag cagctcttcc ggccatactt cttcagtttt
    43621 gaagcggcgg gagaactgga ctaactgcca gagatccgat ttcgcttcgc ccggtgcctg
    43681 tacctgctga cgccagaact gagtacggcg ttcggcgtta ccgtaagcgc cctctttctc
    43741 tacccacatt gcggtcggca ggatcaagtc ggcggccagc gcactgactg tcggatacgg
    43801 atcggagacg atgatgaagt tgcgcggatc gcgccagccc ggcatacgct cttcattgat
    43861 gttcggcccg gcctgcatgt tgttggtaca catggtccag taaacattca gcttgccgtc
    43921 tttcagcgca cggtcttgtg ctaccgcatg cagaccgatt ttcgccggaa tggtgccgct
    43981 cgggatattc cacttcttct cgcagatatc acgatgtttc tcgttagtca ccaccatgtc
    44041 cgcaggcaga cggtgagcaa aggtgcccac ttcacgcgca gtaccacacg cggaaggctg
    44101 cccggtcagg gagaacggac cgcaacccgg ctgggaaatt ttgccggtca gcaggtgcag
    44161 gttgtagacc aggttgttgg cccacacgcc acgagtatgc tggttgaagc ccatcgtcca
    44221 gtaggagatg actttcttgt tcggatcggc atacagctgc gccagttgtt ctaactggtc
    44281 tttcggcacg ccggtcattt cggcagtttt ttccagcgta tactcggcaa cgaaggcttt
    44341 gtaatcttca aagctcatcg gttcggaggc gtcagaaccc ggattcttcg ctgctttttc
    44401 cagcggatgg gtcggacgta aaccgtagcc gatgtccgtc gcccctttgc gcaggttaac
    44461 gtgcttgctg aagaagtcct gatttatcgc attgttttga atgatatagt tggcgatgta
    44521 gttcaggatc accaggtcag attgcggcgt aaagatgatg ccgttatccg ccagctcgaa
    44581 gctacgatgc tggtaggtag aaagcaccgc cacggtgacg ttctggttag agagacgacg
    44641 gttagtgatg cgtgaccaga ggatcgggtg catctccgcc atgttagcgc cccacagcac
    44701 aaacgcgtca gcctgctcga tgtcgtcata gcagcccatc ggctcatcca taccaaaggt
    44761 acgcataaag ccaactactg ccgacgccat acagtgacgt gcgttcgggt cgatgttgtt
    44821 cgaacggaag cccgctttga acagcttgga cgcggcataa ccttcccaga tagtccactg
    44881 accagaaccg aacataccga tcgattccgg tcctttttct ttcagggcgg ttttgaattt
    44941 ctcttccatc acatcgaagg cctgatccca ggtgattggg gtaaattcgc cttctttgtc
    45001 atatttaccg tttttcatac gcagcagcgg ctgcgtcaaa cggtctttac cgtacatgat
    45061 cttgggcagg aaatagccct taatgcagtt caggccacgg ttaaccggtg cgtccgggtc
    45121 gccctgacag gccaccacac gcccctgctg cgttccgacc agaacgccgc aaccagtacc
    45181 gcagaaacgg cacggcgctt tatcccattt aatggcttcc tgctgaccaa caacggcgcg
    45241 ggcaacgccc ggcacgctga gaccggcagc cgccgcagcg gccgcaacgg cgttagcttt
    45301 cataaagcta cgacgactga gtttcatggt gtttcctcac cttgctcttc ctgctggtga
    45361 taaaccagcg acaccgccag cacgccctct acgttgcgta ctgactcaat ggtttggatc
    45421 agcgtttcgc tgtcttctgc ttccaccacc acaatcaact gaccgctcgg cgcgtcgctg
    45481 acagcaactt cacagccggg aaaggcgttc agttgggtgc tgatgtctga aattcgttcg
    45541 cttttggcct gcacgaccag gctgcaaact tgccagttag tgtgcatgga gatactccgc
    45601 agttatggct gataccggac agctggctgc acatgctcca catccgttgc aaagttggct
    45661 attaagttgc ggctggtaga ttccggagag cgtcgggcga aagataattg ccattggctc
    45721 acagctatcc tgacagcggc ggcattcaac tgactgatac gccaggcacg cgtccccaat
    45781 ggtgaactgc aaatcccagg ccctggtgtg gcgcggagaa aatagcgatt cggggcaagc
    45841 ctgcgcgcag gcgtaacaga agctgcactc gttatttttg aaattaacgc tcggatagcc
    45901 gcccgcgccg cgttgcagaa tgttgttttc acaagcatta atacaggcgt cgcaacgggt
    45961 gcaatgggtc agaaaatgag attcatcacc ggaccagggc ggacggatac cgttactggc
    46021 tttgcgccag cgaccagtga gtatgccccg acgggatgca tcaatcttca cattgacctt
    46081 ccatcattaa cgcgctctga aaattgagag cgaccaaata aaccgcataa ttaataagcc
    46141 atttttatag ccgctaagat attaaaggat gtgtcaaaga tgcatacccc gatcggggta
    46201 aaacctgtag caggatcaaa agagtggcgg gaagcgtggc aaaaacgggc ttttgctcac
    46261 atttcaaatg gttataaaca tatttatata gcgattaatt caccagagat atttctgctg
    46321 gtttgctccc tcatcagaat ttaacactaa aagagcaggt aaaattgtct gaatgttctt
    46381 taagttattc ataaagcaaa ttaataaatc tgatgaatat gttaaccttc agcgacatca
    46441 tcggtgaaaa cctataaatg aagaaggaaa gcaaaaaaat gaagaccatt ctacctgcag
    46501 tattgtttgc cgctttcgct accacttccg cctgggcggc agaaagcgtc cagccactgg
    46561 aaaaaatcgc gccttatcca caagctgaaa aagggatgaa gcgtcaggtg attcagttaa
    46621 ccccgcaaga agatgaatct accctgaaag tagaactgtt aatcggtcag acgctggaag
    46681 tcgattgcaa tttgcatcgt ctcggcggga agctggaaag caaaacgctg gaaggctggg
    46741 gctatgatta ttatgtcttt gataaagtta gctcgccggt ttccacgatg atggcctgcc
    46801 cggatggcaa gaaagagaag aaatttgtca ccgcgtatct gggcgatgct ggaatgctgc
    46861 gttacaacag caagctgccg atcgtggtgt atacgccaga caatgtagat gtgaagtacc
    46921 gcgtctggaa ggcggaagag aaaattgaca acgcggtagt tcgctaaact gccgtgaagt
    46981 gcggcacccc gtaggtcaga caaggcggtc acgccgcatc cgacatccaa cg
//
LOCUS       DAPHJE010000022        46112 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPHJE010000022 DAPHJE010000000
VERSION     DAPHJE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.022"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..262)
                     /locus_tag="RH222_001263"
     CDS             complement(<1..262)
                     /locus_tag="RH222_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796316.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIH"
     gene            785..2713
                     /gene="thrS"
                     /locus_tag="RH222_001264"
     CDS             785..2713
                     /gene="thrS"
                     /locus_tag="RH222_001264"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV3796317.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2717..3259
                     /gene="infC"
                     /locus_tag="RH222_001265"
     CDS             2717..3259
                     /gene="infC"
                     /locus_tag="RH222_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV3796318.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3356..3553
                     /gene="rpmI"
                     /locus_tag="RH222_001266"
     CDS             3356..3553
                     /gene="rpmI"
                     /locus_tag="RH222_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV3796319.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3606..3962
                     /gene="rplT"
                     /locus_tag="RH222_001267"
     CDS             3606..3962
                     /gene="rplT"
                     /locus_tag="RH222_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV3796320.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4079..4203
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4085..4129
                     /gene="pheM"
                     /locus_tag="RH222_001268"
     CDS             4085..4129
                     /gene="pheM"
                     /locus_tag="RH222_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV3796321.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4412..5395
                     /gene="pheS"
                     /locus_tag="RH222_001269"
     CDS             4412..5395
                     /gene="pheS"
                     /locus_tag="RH222_001269"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV3796322.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5410..7797
                     /gene="pheT"
                     /locus_tag="RH222_001270"
     CDS             5410..7797
                     /gene="pheT"
                     /locus_tag="RH222_001270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV3796323.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7802..8101
                     /gene="ihfA"
                     /locus_tag="RH222_001271"
     CDS             7802..8101
                     /gene="ihfA"
                     /locus_tag="RH222_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV3796324.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8202..9182
                     /gene="btuC"
                     /locus_tag="RH222_001272"
     CDS             8202..9182
                     /gene="btuC"
                     /locus_tag="RH222_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV3796325.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            9245..9796
                     /gene="btuE"
                     /locus_tag="RH222_001273"
     CDS             9245..9796
                     /gene="btuE"
                     /locus_tag="RH222_001273"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV3796326.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9796..10545
                     /gene="btuD"
                     /locus_tag="RH222_001274"
     CDS             9796..10545
                     /gene="btuD"
                     /locus_tag="RH222_001274"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV3796327.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10623..11087
                     /locus_tag="RH222_001275"
     CDS             10623..11087
                     /locus_tag="RH222_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HDV3796328.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11334..12047
                     /gene="ydiV"
                     /locus_tag="RH222_001276"
     CDS             11334..12047
                     /gene="ydiV"
                     /locus_tag="RH222_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV3796329.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12110..13546
                     /locus_tag="RH222_001277"
     CDS             12110..13546
                     /locus_tag="RH222_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV3796330.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(13550..13741)
                     /gene="hemP"
                     /locus_tag="RH222_001278"
     CDS             complement(13550..13741)
                     /gene="hemP"
                     /locus_tag="RH222_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV3796331.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13873..14919)
                     /gene="aroH"
                     /locus_tag="RH222_001279"
     CDS             complement(13873..14919)
                     /gene="aroH"
                     /locus_tag="RH222_001279"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV3796332.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYYEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15076..15909)
                     /gene="ppsR"
                     /locus_tag="RH222_001280"
     CDS             complement(15076..15909)
                     /gene="ppsR"
                     /locus_tag="RH222_001280"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV3796333.1"
                     /translation="MENAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEVLYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16242..18620
                     /gene="ppsA"
                     /locus_tag="RH222_001281"
     CDS             16242..18620
                     /gene="ppsA"
                     /locus_tag="RH222_001281"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV3796334.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18677..20323)
                     /gene="fadK"
                     /locus_tag="RH222_001282"
     CDS             complement(18677..20323)
                     /gene="fadK"
                     /locus_tag="RH222_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV3796335.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(20380..20673)
                     /locus_tag="RH222_001283"
     CDS             complement(20380..20673)
                     /locus_tag="RH222_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV3796336.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20670..21959)
                     /locus_tag="RH222_001284"
     CDS             complement(20670..21959)
                     /locus_tag="RH222_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3796337.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMELAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22015..22953)
                     /locus_tag="RH222_001285"
     CDS             complement(22015..22953)
                     /locus_tag="RH222_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDV3796338.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22973..23737)
                     /locus_tag="RH222_001286"
     CDS             complement(22973..23737)
                     /locus_tag="RH222_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV3796339.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLTTDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            24053..24964
                     /locus_tag="RH222_001287"
     CDS             24053..24964
                     /locus_tag="RH222_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3796340.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVDCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(25007..26158)
                     /locus_tag="RH222_001288"
     CDS             complement(25007..26158)
                     /locus_tag="RH222_001288"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV3796341.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26172..27767)
                     /locus_tag="RH222_001289"
     CDS             complement(26172..27767)
                     /locus_tag="RH222_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase subunit
                     alpha/beta"
                     /protein_id="HDV3796342.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKPIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSPKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(27912..28670)
                     /gene="aroD"
                     /locus_tag="RH222_001290"
     CDS             complement(27912..28670)
                     /gene="aroD"
                     /locus_tag="RH222_001290"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV3796343.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(28701..29567)
                     /gene="ydiB"
                     /locus_tag="RH222_001291"
     CDS             complement(28701..29567)
                     /gene="ydiB"
                     /locus_tag="RH222_001291"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV3796344.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(29579..30844)
                     /locus_tag="RH222_001292"
     CDS             complement(29579..30844)
                     /locus_tag="RH222_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796345.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(31071..32285)
                     /locus_tag="RH222_001293"
     CDS             complement(31071..32285)
                     /locus_tag="RH222_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796346.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYLCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(32385..32741)
                     /locus_tag="RH222_001294"
     CDS             complement(32385..32741)
                     /locus_tag="RH222_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV3796347.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(32877..32984)
                     /gene="rprA"
                     /locus_tag="RH222_001295"
     ncRNA           complement(32877..32984)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RH222_001295"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(33170..34282)
                     /gene="ydiK"
                     /locus_tag="RH222_001296"
     CDS             complement(33170..34282)
                     /gene="ydiK"
                     /locus_tag="RH222_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV3796348.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQFGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            34671..37727
                     /locus_tag="RH222_001297"
     CDS             34671..37727
                     /locus_tag="RH222_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV3796349.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            37724..38134
                     /gene="menI"
                     /locus_tag="RH222_001298"
     CDS             37724..38134
                     /gene="menI"
                     /locus_tag="RH222_001298"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV3796350.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            38234..38422
                     /locus_tag="RH222_001299"
     CDS             38234..38422
                     /locus_tag="RH222_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV3796351.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            38970..39338
                     /gene="sufA"
                     /locus_tag="RH222_001300"
     CDS             38970..39338
                     /gene="sufA"
                     /locus_tag="RH222_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV3796352.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            39347..40834
                     /gene="sufB"
                     /locus_tag="RH222_001301"
     CDS             39347..40834
                     /gene="sufB"
                     /locus_tag="RH222_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV3796353.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            40844..41590
                     /gene="sufC"
                     /locus_tag="RH222_001302"
     CDS             40844..41590
                     /gene="sufC"
                     /locus_tag="RH222_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV3796354.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIYMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            41565..42836
                     /gene="sufD"
                     /locus_tag="RH222_001303"
     CDS             41565..42836
                     /gene="sufD"
                     /locus_tag="RH222_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV3796355.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            42833..44053
                     /gene="sufS"
                     /locus_tag="RH222_001304"
     CDS             42833..44053
                     /gene="sufS"
                     /locus_tag="RH222_001304"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV3796356.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            44066..44482
                     /gene="sufE"
                     /locus_tag="RH222_001305"
     CDS             44066..44482
                     /gene="sufE"
                     /locus_tag="RH222_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV3796357.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            44631..45635
                     /gene="ldtE"
                     /locus_tag="RH222_001306"
     CDS             44631..45635
                     /gene="ldtE"
                     /locus_tag="RH222_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV3796358.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLGR
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(45699..45935)
                     /gene="lpp"
                     /locus_tag="RH222_001307"
     CDS             complement(45699..45935)
                     /gene="lpp"
                     /locus_tag="RH222_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV3796359.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT        11674 a        11181 c        11922 g        11335 t
ORIGIN      
        1 catggatggg tgaagtggca aagttaatgg taaaaagggc agtagaataa caattttgtg
       61 atatttttcg acagtttgcg gtaaattcct gagtaacaca gtatgacagc atcgaactta
      121 agtcagaggt atcaagtagg gtgatgagtt ggcgcgcaaa ttgattaata ggctctgatg
      181 aaggcgttgg tatgtcgcta tcgatgtgca ttctatgagg agacttataa tgagttctga
      241 taataatgtc gttttgactc ataatctatt cgccttatat atgtctggag gttttgagta
      301 tgatgattaa ttattatcgc cagaacgaaa tgtgcacgaa aaattgctat aagaattgtg
      361 tcacttttat cattgtgata tgaccagttc gtcattaatc ttatgatcgt ttaaatacta
      421 ccgccaaatc atgcaacgaa ctttgctgga ttcagtttat gctgctgtaa atccgctcgc
      481 agtaaacctt tcagacgctc cgtgatgtta tcagttgttc tttaagcgtt ttgctggtgt
      541 actcactaca aacgaattgc gaatcgaatc aatgtgaaac ggaaaggtac aatctccctt
      601 tttttagtgt taacatcgct caaccgggtg gaaggtgaga ggttaatgtc tttcatttgt
      661 gtacctgatt gcgaaccaat ttagcatttg ttggctagat ggtttcgcaa tgaactgtta
      721 ataaacaaat ttttctttgt atgtgatctt tcgtgtgggt caccactgca aataaggata
      781 taaaatgcct gttataactc ttcctgatgg cagccaacgc cattacgacc acgctgtaag
      841 ccccatggat gttgcgctgg acattggtcc aggtctggcg aaagcctgta tcgcagggcg
      901 cgttaatggc gaactggttg atgcttgcga tctgattgaa aacgacgcac aactgtcgat
      961 cattaccgcc aaagacgaag aaggtctgga gatcattcgt cactcctgtg cgcacctgtt
     1021 agggcacgcg attaaacaac tttggccgca taccaaaatg gcaatcggcc cggttattga
     1081 caacggtttt tattacgacg ttgatcttga ccgcacgtta acccaggaag atgtcgaagc
     1141 actcgagaag cggatgcatg agcttgctga gaaaaactac gacgtcatta agaagaaagt
     1201 cagctggcac gaagcgcgtg aaactttcgc caaccgtggg gagagctaca aagtctccat
     1261 tcttgacgaa aacatcgccc atgatgacaa gccaggtctg tacttccatg aagaatatgt
     1321 cgatatgtgc cgcggtccgc acgtaccgaa catgcgtttc tgccatcatt tcaaactaat
     1381 gaaaacggca ggggcttact ggcgtggcga cagcaacaac aaaatgttgc aacgtattta
     1441 cggtacggcg tgggcagaca aaaaagcact taacgcttac ctgcagcgcc tggaagaagc
     1501 cgcgaaacgc gaccaccgta aaatcggtaa acaactcgac ctgtatcata tgcaggaaga
     1561 agcgccgggt atggtattct ggcacaacga cggctggacc attttccgtg aactggaagt
     1621 gtttgttcgt tctaaactga aagagtacca gtatcaggaa gttaaaggtc cgttcatgat
     1681 ggaccgtgtc ctgtgggaaa aaaccggtca ctgggacaac tacaaagatg caatgttcac
     1741 cacgtcttct gagaaccgtg aatactgcat taagccgatg aactgcccgg gtcacgtaca
     1801 aattttcaac caggggctga agtcttatcg cgatctgccg ctgcgtatgg ccgagtttgg
     1861 tagctgccac cgtaatgagc cgtcaggttc gctgcatggc ctgatgcgcg tgcgtggatt
     1921 tacccaggat gacgcgcata tcttctgtac tgaagaacaa attcgcgatg aagttaacgg
     1981 atgtatccgt ttagtctatg atatgtacag cacttttggc ttcgagaaga tcgtcgtcaa
     2041 actctccact cgtcctgaaa aacgtattgg cagcgacgaa atgtgggatc gtgctgaggc
     2101 ggacctggcg gttgcgctgg aagaaaacaa catcccgttt gaatatcaac tgggtgaagg
     2161 cgctttctac ggtccgaaaa ttgaatttac cctgtatgac tgcctcgatc gtgcatggca
     2221 gtgcggtaca gtacagctgg acttctcctt gccgtctcgt ctgagcgcct cctatgtggg
     2281 tgaagacaac gagcgtaagg taccggtaat gattcaccgc gcaattcttg ggtcgatgga
     2341 acgtttcatc ggtatcctga ccgaagaatt cgctggtttc ttcccgacct ggcttgcgcc
     2401 ggttcaggtt gttatcatga atattaccga ttcacagtct gattacgtta acgaattgac
     2461 gcaaaaacta tcaaatgcgg gcattcgtgt taaagcagac ttgagaaatg agaagattgg
     2521 ctttaaaatc cgcgagcaca ctttgcgtcg cgtcccatat atgctggtct gtggtgataa
     2581 agaggtggaa tcaggcaaag ttgccgttcg cacccgccgt ggtaaagacc tgggaagcat
     2641 ggacgtaaat gaagtgatcg agaagctgca acaagagatt cgcagccgca gtcttaaaca
     2701 attggaggaa taaggtatta aaggcggaaa acgagttcaa acggcgcgcc ctaaccgtat
     2761 caatggcgaa attcgcgccc aggaagttcg cttaacaggt ctggaaggcg agcagcttgg
     2821 tattgtgagt ctgagagaag ctctggagaa agcagaagaa gccggagtag acttagtcga
     2881 gatcagccct aacgccgagc cgccggtttg tcgtataatg gattacggca aattcctcta
     2941 tgaaaagagc aagtcttcta aggaacagaa gaaaaagcaa aaagttatcc aggttaagga
     3001 aattaaattc cgtcctggta cagatgaagg cgactatcag gtaaaactcc gcagcctgat
     3061 tcgctttctc gaagagggtg ataaagccaa aatcacgctg cgtttccgcg gtcgtgagat
     3121 ggcgcaccag caaatcggta tggaagtgct taatcgcgtg aaagacgatt tgcaagaact
     3181 ggcggtggtc gaatccttcc caacgaagat cgaaggccgc cagatgatca tggtgctcgc
     3241 tcctaagaag aaacagtaag gccttcaagt agcactgtct gtggagcctt cgggcttcac
     3301 aggcattgtt cgcctttgtt tcgtttatta acaatgcgaa gtggaagtta ttaaaatgcc
     3361 aaaaattaag accgtacgcg gtgctgctaa gcgcttcaaa aaaaccggta aaggtggttt
     3421 taagcacaag cacgctaacc tgcgtcacat tctgaccaaa aaagcgacca aacgtaaacg
     3481 tcacctgcgt ccgaaagcca tggtttccaa aggcgatctg ggcctggtaa tcgcgtgcct
     3541 gccgtacgca taagccgtta acgtttttaa ctttttaatt agaatataga tacaggagag
     3601 cacatatggc tcgcgtaaaa cgtggtgtta ttgcacgcgc acgtcacaag aaaattttga
     3661 aacaagctaa aggctactac ggtgcgcgtt ctcgcgtata ccgcgttgcc ttccaggctg
     3721 ttatcaaagc tggtcagtat gcttaccgtg accgtcgtca acgtaagcgt cagttccgtc
     3781 aactgtggat tgcgcgtatc aacgcagcag cacgtcagaa cggtatttct tacagcaaat
     3841 tcatcaatgg cctgaaaaaa gcctctgttg aaatcgaccg taagatcctg gctgatatcg
     3901 cagtattcga caaagtagcg ttcaccgctc tggttgaaaa agcgaaagca gctctggcat
     3961 aagccagttg aaagagggag ctagtctccc tcttttcgtt tcaacgccat caaaacattg
     4021 acttttatcg ccgtagcctt ttcaataaag gtcttttgaa gagtaccaaa aggtaacgca
     4081 agcaatgaat gctgctattt tccgcttctt tttttacttt agcacctgaa tccaggaggc
     4141 tagcgcgtga gaagagaaac ggaaaacagc gcctgaaagc ctcccagtgg aggctttttt
     4201 tgtatgcgcg ttttgaaaaa taaaaagcca gtctatcagg cgtaattgtc gcagccagtc
     4261 tggacacggc cagcgcggag aaaccggagc gtaattaagt acgtgaggat ttcgagcact
     4321 gcccgggacc aaaatggcaa gtaaaatagc ctgatgggat aggctctaag tccaacgaac
     4381 cagtgtcacc actgacacaa tgaggaaaac catgtcacat ctcgcagaac tggttgccag
     4441 tgcgaaggcg gccattagcc aggcgtcaga tgttgccgcg ttagataatg tgcgcgtcga
     4501 atatttgggt aaaaaagggc acttaaccct tcagatgacg accctgcgtg agctgccgcc
     4561 agaagagcgt ccggcagctg gtgcggttat caacgaagcg aaagagcagg ttcagcaggc
     4621 gctgaatgcg cgtaaagcgg aactggaaag cgctgcactg aatgcgcgtc tggcggcgga
     4681 aacgattgat gtctctctgc caggtcgtcg cattgaaaac ggcggtctgc atccggttac
     4741 ccgtaccatc gaccgtatcg aaagtttctt cggtgagctt ggctttaccg tggcaaccgg
     4801 gccggaaatc gaagacgatt atcataactt cgatgctctg aacattcctg gtcaccaccc
     4861 ggcgcgcgct gaccacgaca ctttctggtt tgacgctacc cgcctgctgc gtacccagac
     4921 ctctggcgta cagatccgca ccatgaaagc ccagcagcca ccgattcgta tcatcgcgcc
     4981 tggccgtgtt tatcgtaacg actacgacca gactcacacg ccgatgttcc atcagatgga
     5041 aggtctgatt gttgatacca acatcagctt taccaacctg aaaggcacgc tgcacgactt
     5101 cctgcgtaac ttctttgagg aagatttgca gattcgcttc cgtccttcct acttcccgtt
     5161 taccgaacct tctgcagaag tggatgtcat gggtaaaaac ggtaaatggc tggaagtgct
     5221 gggctgcggg atggtgcatc cgaacgtgct gcgtaacgtt ggcatcgacc cggaagttta
     5281 ctctggtttc gccttcggga tggggatgga gcgtctgact atgttgcgtt acggcgtcac
     5341 cgacctgcgt tcattcttcg aaaacgatct gcgtttcctc aaacagttta aataaggcag
     5401 gaatagatta tgaaattcag tgaactgtgg ttacgcgaat gggtgaaccc ggcgattgat
     5461 agcgatgcgc tggcaaatca aatcactatg gcgggcctgg aagttgacgg tgtagaaccg
     5521 gttgccggta gcttccacgg cgtggtagtt ggtgaagtgg ttgagtgtgc gcagcatccg
     5581 aacgctgaca aactgcgtgt gacaaaagtg aatgttggcg gcgatcgcct gctggacatc
     5641 gtctgcggtg cgccaaactg ccgtcagggc ctgcgtgtgg cggtagcgac cattggtgct
     5701 gttctgccgg gtgatttcaa aattaaagcg gcgaaactgc gtggtgaacc gtctgaaggg
     5761 atgctgtgct ccttttctga gctgggaatt tctgacgatc atagcggcat tatcgagctg
     5821 cctgcggatg cgccgattgg caccgacatc cgtgaatacc tgaaacttga tgacaacacc
     5881 atcgaaatca gcgtgacgcc aaaccgtgcc gactgcttag gcatcattgg tgttgcgcgt
     5941 gacgttgccg tgctgaacca gctgccgctg gttgaaccgg aaatcgttcc ggttggtgcg
     6001 accatcgacg acacgctgcc gattacagtc gaagcgccgg aagcctgccc gcgttatctt
     6061 ggccgtgtgg taaaaggcat taacgttaaa gcgccaactc cgctgtggat gaaagaaaaa
     6121 ctgcgtcgtt gcgggatccg ttctatcgat gcagttgttg acgtcaccaa ctatgtgctg
     6181 ctcgaactgg gccagccgat gcacgctttc gataaagatc gcattgaagg cggcattgtg
     6241 gtgcggatgg caaaagaggg cgaaacgctg gtgctgcttg atggcactga agcgaagctg
     6301 aatgctgaca ctctggtcat cgccgaccac aacaaggcgc tggcgatggg cggcatcttt
     6361 ggtggcgaac actctggcgt gaatgacgaa acgcaaaacg tcctgctgga atgcgctttc
     6421 ttcagcccgc tgtctatcac cggtcgtgct cgtcgtcatg gcctgcatac tgatgcgtct
     6481 caccgttatg agcgtggcgt tgatccggca ctgcagcaca aagcgatgga acgtgcgacc
     6541 cgtctgctga tcgacatctg cggtggtgag gctggtccgg taattgatat caccagcgaa
     6601 gcaacgctgc cgaagcgtgc aaccatcact ttacgtcgta gcaaactgga tcgcctgatc
     6661 ggccatcata ttgcggatga gcaggtaact gacattctgc gtcgtctcgg ctgcgaagtg
     6721 accgaaggca aagacgagtg gcaggcagtt gcgccgagct ggcgtttcga tatggagatt
     6781 gaagaagatc tggtcgaaga agtcgcgcgt gtttacggct acaacaacat cccggatgag
     6841 ccggtacagg caagcctgat tatgggtact caccgtgaag ctgacctgtc gctcaagcgc
     6901 gtgaaaacgc tgctcaacga caaaggctat caggaagtga tcacctacag cttcgttgat
     6961 ccgaaagtgc agcagatgat ccatccaggc gttgaagcct tactgctgcc aagcccgatc
     7021 tctgttgaaa tgtcagcaat gcgtctttct ctgtggaccg gcctgctggc aaccgtggtg
     7081 tacaaccaga accgtcagca gaaccgtgtg cgcattttcg aaagcggtct gcgtttcgta
     7141 ccagatactc aagcaccgtt gggcattcgt caggatctga tgttagccgg tgtgatttgc
     7201 ggtaaccgtt acgaagagca ctggaacctg gcaaaagaga ccgttgattt ctatgatttg
     7261 aaaggcgatc ttgaatccgt tctcgacctg accggtaaac tgaatgaggt tgagttccgt
     7321 gcagaagcga atccggcact gcatccgggg caatccgcag cgatttatct gaaaggtgaa
     7381 cgtattggtt ttgttggggt tgttcatcct gaactggaac gtaaactgga tcttaacggt
     7441 cgcactctgg tgttcgaact ggagtggaac aagctcgcag accgcgtggt gcctcaggcg
     7501 cgcgagattt ctcgcttccc ggcgaaccgt cgtgacatcg cggtggtggt cgcagaaaac
     7561 gttcccgcag cggatatttt atccgaatgt aagaaagttg gcgtaaatca ggtagttggc
     7621 gtaaacttat ttgacgtgta ccgcggtaag ggtgttgcgg aggggtataa gagcctcgcc
     7681 ataagcctga tcctgcaaga taccagccgt acactcgaag aagaggagat tgccgctacc
     7741 gtcgccaaat gtgtagaggc attaaaagag cgattccagg catcattgag ggattgaacc
     7801 tatggcgctt acaaaagctg aaatgtcaga atatctgttt gataagcttg ggcttagcaa
     7861 gcgggatgcc aaagaactgg ttgaactgtt tttcgaagag atccgtcgcg ctctggaaaa
     7921 cggcgaacag gtgaaactct ctggttttgg taacttcgat ctgcgtgata agaatcaacg
     7981 cccgggacgt aacccgaaaa cgggcgagga tattcccatt acagcacggc gcgtggtgac
     8041 cttcagaccc gggcagaagt taaaaagccg ggtcgaaaac gcttcgccca aagacgagta
     8101 atctgatcta actaaaaagg ccgctctgcg gccttttttc ttttcactgt cgaagagtca
     8161 ccgtaaaatc aacgccatga cacttcagca gaacggatac catgctgaca cttgcccgcc
     8221 aacaacagcg acaaaatatt cgctggttat tatgcctgtc agttttgatg ctgctggcgc
     8281 ttctcttaag cctttgcgcc ggtgaacaat ggatctcgcc aggtgactgg tttactcctc
     8341 gtggcgaact gttcgtctgg caaattcgcc tgccacgtac gctggctgta ttgctggttg
     8401 gtgcggcgct ggctatatcc ggcgctgtaa tgcaggcgtt gtttgaaaat cctctggcag
     8461 aacctggact acttggcgtc tctaacggcg caggcgtggg gcttatcgcc gcggtattgc
     8521 ttgggcaagg gcaactcccc aactgggcgc tagggctgtg tgcgattgct ggcgcgctta
     8581 tcatcacttt aatactctta cgtttcgccc gtcgtcatct ttcgaccagt cggttattgc
     8641 tggctggcgt tgcattaggg attatctgta gcgcactaat gacgtgggct atctactttt
     8701 ccacctcagt tgatttgcgt cagctgatgt actggatgat gggcggtttt ggcggcgtag
     8761 actggcggca aagctggctg atgctggcat tgatccccgt gttgttgtgg atctgttgtc
     8821 agtccaggcc gatgaatatg ttagcacttg gcgagatctc ggcgcggcaa ctgggtttac
     8881 ccctgtggtt ctggcgcaat gtgctggtgg cagcgaccgg ctggatggtt ggcgtcagtg
     8941 tggcgctggc gggtgctatc ggctttattg gtctggtgat cccacatatt ctccggctgt
     9001 gtggtttaac cgatcatcgc gtattacttc ccggctgcgc gctggcaggg gcgagcgcat
     9061 tgctgctggc cgatattgta gcgcgcctgg cattagctgc cgcagagctg cctattggcg
     9121 tggtcaccgc aacgttgggt gcgccggtgt ttatctggtt attgttaaaa gcaagacgtt
     9181 agccgcaaaa agacggtcta tgattaaaag ctaagatttt tactgaccac acccaggaga
     9241 aacgatgcaa gattccattc tgacgaccgt agtgaaagat atcgacggtg aagtgaccac
     9301 gctggagaag tacgccggta atgtgctgtt gattgtcaat gtcgcctcaa agtgtggctt
     9361 aacgccgcaa tatgagcagt tggagaatat tcagaaagcc tgggccgatc gaggttttgt
     9421 ggtgctggga ttcccgtgca accagtttct ggaacaagaa ccgggcagcg atgaagagat
     9481 taaaacttac tgtaccacca catggggggt gacgttcccg atgttcagta agattgaagt
     9541 taatggcgaa ggacgccatc cgctgtatca aaaattgatt gccgcagcgc cgaccgcagt
     9601 cgcgccggaa gagagcggat tctatgcccg tatggtcagc aaaggccgtg caccgctgta
     9661 cccggatgat attttatgga attttgaaaa attcctggtt ggcagggacg gaaaagtcat
     9721 ccagcgtttt tccccggata tgacgccgga agatcccatt gtgatggaaa gcattaaact
     9781 ggcgttggca aaataatgtc tattgtgatg cagttacaag atgttgcgga atctacccgc
     9841 ctggggccgc tttctggcga ggttcgggct ggggagatcc tgcacctggt ggggccgaat
     9901 ggcgcgggta agagtacctt actggcgcga atggccggaa tgaccagcgg taagggaagc
     9961 attcagttcg cggggcaacc actggaagca tggtccgcaa caaaactcgc gctgcatcgc
    10021 gcctatcttt cacaacagca gacgccgccg tttgcaatgc cggtctggca ctacctgaca
    10081 ctgcatcagc acgataaaac gcgtaccgaa ctactgaatg atgtcgcagg ggcgctggct
    10141 cttgatgaca aactcggacg tagcaccaat caactttccg gcggtgaatg gcaacgcgta
    10201 cgtcttgctg cggtggtgtt gcaaatcaca ccacaagcca atcccgcagg ccaattgctg
    10261 cttcttgatg agccgatgaa cagtcttgat gttgcgcaac aaagtgcgtt agacaaaatt
    10321 ctgagcgcgc tgtgtcagca aggactggcg attgtgatga gcagtcacga tctcaaccac
    10381 acattgcgtc atgcgcatcg ggcgtggttg ctaaaaggtg gaaaaatgct ggccagtgga
    10441 cgcagggaag aggtgctcac gccgccaaat ctggcgcagg cctatgggat gaattttcgc
    10501 cgtctggata tcgaaggtca cagaatgctg atttcgacca tctgatttca ttagtatctt
    10561 gaaaatcggg taattacgac gctaaattaa tgcaagaata aaaaacagag gaaccgtacg
    10621 gaatgcgttt ctgccttatt ttgatcacag cactgcttct ggccgggtgt agccaccata
    10681 aagcaccgcc gccaaatgcc agactttctg attcgattac cgttattgcc ggtttgaacg
    10741 accagctaca aagctggcat ggcacgccgt atcgttatgg tggcatgacg cggcgcggtg
    10801 tggactgttc gggatttgtg gttgtgacga tgcgcgatcg tttcgatttg cagctgcccc
    10861 gtgaaaccaa acaacaagcc tctattggca cacaaattga taaagacgag ttgctgccag
    10921 gtgacctggt ctttttcaaa acggggtccg gacaaaatgg tttgcatgta ggtatttacg
    10981 ataccaacaa ccaatttatc cacgcctcta ccagcaaggg agtgatgcgt tcctcacttg
    11041 ataatgtcta ttggcagaaa aatttctggc aggcgagacg aatctagtat gtcgaaaaat
    11101 ggacggcgat gatacatcgc cgtccattta ctcctgaata cagtagtatt ttgcagacaa
    11161 aaattccgta aatggattat tcgtttataa tcaggataat ctaaaaaaag gacccctgaa
    11221 aaagtggtta acatggaaca aatgaaagtt actccttctg taattattat gtcttatggc
    11281 ctcatccctt ttagccggat actgaaaaac atccttcgag agggacggtt accatgaaga
    11341 tttttttgga gaatctttat cattctgatt gctatttcct gccgataaga gacaaccagc
    11401 aagtccttgt cggcgttgag ttaattaccc atttctccag tgaagatggc acggtgcgta
    11461 tcccgacgag tcgggttata gcgcaactta ccgaggagca acattggcaa ttattttcag
    11521 agcagttgga attactaaaa tcatgccaac atttttttat tcaacataaa ctctttgcct
    11581 ggcttaattt aacgccacaa gtggctacat tattattaga gagggataat tacgcaggcg
    11641 aactattaaa atatccgttt attgaactgt tgattaatga gaattacccg catctcaatg
    11701 aaggaaaaga taatcgcggt cttttatcct tatcgcaagt gtatcccctt gtgttaggta
    11761 atttgggagc aggcaatagc acaatgaaag cggtttttga tggtcttttc acccgcgtca
    11821 tgctggataa aagttttatt cagcaacaaa tcacgcaccg ctccttcgaa ccgtttattc
    11881 gtgcgatcca ggcgcaaatt tccccttgtt gcaattgcat cattgcggga ggcattgata
    11941 cggcagaaat attggcacag attaccccct tcgatttcca tgctctccag ggatgcctgt
    12001 ggcctgccgt tcccataaat cagataacga cgttggttca gcgataaccc ttctgtttgc
    12061 tggtgtttaa gacgagagta accgtctaca ctatcaaaca ggaggatcta tgaccctgtc
    12121 ttttgttacc cgctggcgcg atgaattgcc agaaacctat acagcacttt cccctacgcc
    12181 tttaaataat gcccggctga tttggcataa taccgaactg gctaacacgc tgagtattcc
    12241 atcgtcgctg tttaaaaatg gcgcaggtgt ctggggcggc gaagccctac tgcctggtat
    12301 gtcaccactg gcgcaggttt acagtggtca tcagttcggc gtctgggcgg gccaactggg
    12361 tgatgggcgc ggcattttac tcggcgaaca actgcttgct gatggcacta caatggactg
    12421 gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg ggtgatggac gggcggtttt
    12481 acgttcgacg atacgagaaa gtctcgccag tgaggcgatg cattatctgg gcattccgac
    12541 gacccgcgcg ttaagtatcg tcaccagcga ttcgccagtg tatcgggaaa cggcggagcc
    12601 aggcgcgatg ctgatgcgtg tggcaccaag tcatctgcgc tttggtcatt tcgaacattt
    12661 ttactaccgc cgcgagtcgg aaaaggttcg tcagttagct gactttgcta ttcgtcatta
    12721 ctggtcacat cttgaagatg atgaggacaa ataccgtctc tggtttagcg atgttgtcgc
    12781 acgtaccgcg tcgttaattg cccaatggca gacggtcggc tttgctcatg gggtgatgaa
    12841 taccgacaac atgtcgctgc tggggctgac gcttgattac gggccgtttg gttttcttga
    12901 tgattacgaa cccggtttta tttgtaatca ctcggatcat caagggcgtt acagctttga
    12961 taatcaacct gccgtcgcgt tgtggaattt acagcgtctg gcgcagacat tgtcgccatt
    13021 tgttgccgta gatgccctga atgaggccct ggacagctat cagcaggttt tgttgacgca
    13081 ttatggagaa cggatgcggc agaaactggg cttcatgacg gagcaaaaag aggataacgc
    13141 gctactgaat gaattattca gtctgatggc gcgagagcgc agcgattata cccgcacatt
    13201 ccgcatgctg agtctgaccg agcagcacag cgcggcgtca ccgctacgtg atgagtttat
    13261 tgatcgtgcg gcatttgatg actggtttgc ccgttatcgg gggcgtttgc aacaagacga
    13321 ggttagcgat agtgagcgtc agcagctgat gcaaagcgtt aacccagctc tggtgttgcg
    13381 caactggttg gcgcaacggg cgattgaagc ggcagaaaag ggtgatatga cggaattgca
    13441 ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga gctgatgact atgtcagtcg
    13501 tccacctgac tggggtaaac ggctggaagt cagctgctcg agttaaaggc tacttggtca
    13561 acagcagctt gccagcctga gttttacgga gcagatattc ttgcccgtca tgatcgataa
    13621 tcagtttgcc atccggacct aacagtgtct ggctggaaat tcgccgaata ggctgcgggg
    13681 aagcggtctt gtgattggca tccgtttcag gcgtgagttt tctgctatcc gtataacgca
    13741 taaaaattaa taataattga tataacaatg attcttatta tcaatattat ggctgaccag
    13801 agcaagcagt aatgtgctga gaagacaaca agttgcgcag aaatgcccat cccatcagga
    13861 atgggcacgc attcagaagc gggtatctac cgcagaggcg agtttttcga ccaggcgttc
    13921 ggtatcttcc cagcccagac acgggtcggt aatggattga ccgtaagtga gcggctgacc
    13981 gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc gccataattc cagcaatcgc
    14041 cgtagagcca ttgcggattt gctgacaaat atcctcacaa acttctaact ggcgacggtg
    14101 ctgcttctgg cagttaccgt ggctgaaatc caccaccaga tgttcaggta aatcaaactc
    14161 gtgcagcgta tcgcaggctg cggcgatatc atcggcatga taattcggtt ttttgccgcc
    14221 acgcataata atgtggccat acgggttgcc gctggtctga tagatggtca tctgaccatt
    14281 tttgtctggc gagagaaaca tatggctggc gcgggctgcg cggatagcat ccacggcaat
    14341 ccgcgtgttg ccatcggtac catttttaaa acctaccgga caggagagtg ccgaagccat
    14401 ttcacggtgg atctgacttt cggtagtacg tgcgccaatc gctccccaac tgattaaatc
    14461 agcaataaac tgaccggtca ccatatcgag gaactcagtc gcggtaggga cacccagctc
    14521 atttacctgt aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc gatagctgcc
    14581 gtttaaatct ggatcggaga ttagcccttt ccagccgaca acagttcggg gtttttcata
    14641 ataggtgcgc attacgattt caagccgtga ctggtactgg ttgcgcagcg actgcagacg
    14701 ggtggcgtat tccattgcag cggtgagatc gtggatcgag caggggccaa taatgaccaa
    14761 cagtcgctta tcttcaccat tcagtatttt ttcaattctg cggcgggagt cggtgacatg
    14821 ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg ttaccaggct
    14881 ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt acaagtctca gaaatttgtt
    14941 gcttctacgg caggactacc gggaagtgat gcgcatcacg ataaaccaat cgcagctgat
    15001 ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc atgataacaa aaaaacaagt
    15061 taatgcacta gttctctagt acattcgacg gctaaggccc atgatatcga ggatcttggt
    15121 ggcaatctct tctaccgaat aattggtact gttaatccac gggatctgat ttttacggta
    15181 caacacttcc acttccgcga cttccatcct gcactgacga agcgaggcat agcgactgtt
    15241 ctcccgacgt tcctcgcgaa tcgccgccag tcgttccggg tcgatagtca ggccgaacaa
    15301 tttatgctga agcggtttga gcgacgcggg tagcaccaga ttatccatat cgtcggcaat
    15361 aaaggggtag tttgccgcgc ggataccaaa ctgcattgcc agatacagac tggtgggggt
    15421 tttaccacag cgagaaacac cgagcaggat cacctgagcc tggtccagat tgcgcaacga
    15481 aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca atgcgcgcat catatttatt
    15541 gagattatta gggttaaggc catgggtacg atgagcaatc ggcgttggat ccagtttcat
    15601 ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc tggcaaaaac cttcactttg
    15661 caagatgatg gcgcgaatct ccggcaacac gatggagtag aagaccagcg ggcgcacacc
    15721 tgtctggtga taaattgcgt caatctgatc cttcactgcc cgtgcacggc tctcattttc
    15781 gacaaacggc agcgtaatgc tgctgatagt gacgggaaat tgtgacatta ctgcgtgtcc
    15841 taatacctcc gcagttattg ccgtaccatc agaaatataa aaaacgtggc gatcaacagc
    15901 attttccatt ttgttcttcc cgtgatgcag acataattct atgaaagcat aaattaaaaa
    15961 cgcacagaag cgtaggacgt aatgtctggt ttataaaatg aaccttcaat tttatttttt
    16021 atgaaaacag catttcattt ttatggtttc gtttataccg atggtttatg tggaaattgt
    16081 cgaatagagc aggtttgcgc aacgctggga tcagtcttaa aaagtaaaaa aatatatttg
    16141 cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc aaagataaat gcgcagaaat
    16201 gtgtttctca aaccgttcat ttatcacaaa aggattgttc gatgtccaac aatggctcgt
    16261 caccgctggt gctttggtat aaccaactcg gcatgaatga tgtagacagg gttgggggca
    16321 aaaatgcctc cctgggtgaa atgattacta atctttccgg aatgggcgtt tccgttccga
    16381 atggtttcgc cacaaccgcc gacgcgttta accagtttct ggaccaaagc ggcgtaaacc
    16441 agcgcattta tgaactgctg gataaaacgg atattgacga tgttacccag cttgcgaaag
    16501 cgggcgcgca aatccgccag tggattatcg acactccctt ccagcctgag ctggaaaatg
    16561 ccatccgcga agcttatgca cagctttccg ccgatgacga aaacgcctct tttgcggtgc
    16621 gctcctccgc tactgcagaa gatatgccgg acgcttcctt tgccggtcag caggaaacct
    16681 tcctcaacgt tcagggtttt gacgccgttc tcgtggcagt gaaacatgta tttgcttctc
    16741 tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg ttacgatcac cgtggcgtgg
    16801 cgctctccgc cggtgttcaa cggatggtgc gctctgacct cgcatcttct ggcgtgatgt
    16861 tctccattga taccgaatcc ggctttgacc aggtggtgtt tatcacttcc gcatggggcc
    16921 ttggtgaaat ggtcgtgcag ggcgcggtta acccggatga gttttacgtc cataaaccga
    16981 cacttgcagc gaatcgcccg gctatcgtgc gtcgcaccat ggggtcgaaa aaaatccgca
    17041 tggtttacgc gccgacccag gagcacggca agcaggttaa aatcgaagac gtaccgcagg
    17101 aacagcgtga catcttctcg ctgaccaacg aagaagtgca ggaactggca aaacaggccg
    17161 tacaaattga gaaacactac ggtcgcccga tggatattga gtgggcgaaa gatggccaca
    17221 ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt gcgctcacgc ggtcaggtca
    17281 tggagcgtta tacgctgcat tcacagggta agattatcgc cgaaggccgt gctatcggtc
    17341 atcgcatcgg cgcgggtccg gtgaaagtca tccatgacat cagcgaaatg aaccgcatcg
    17401 aacctggcga cgtgctggtc actgacatga ccgacccgga ctgggaaccg atcatgaaga
    17461 aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg tcacgcggcg atcatcgctc
    17521 gtgaactggg cattccggcg gtagtgggct gtggtgatgc aacagaacgg atgaaagacg
    17581 gtgagaacgt cactgtttct tgtgccgaag gtgataccgg ttacgtctat gcggagttgc
    17641 tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc ggatctgccg ttgaaagtga
    17701 tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc ctgcctgccg aacgaaggcg
    17761 tgggccttgc gcgtctggaa tttatcatca accgtatgat tggcgtccac ccacgcgcac
    17821 tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga aatccgcgag atgatgaaag
    17881 gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac tgaagggatc gcgacgctgg
    17941 gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc tgattttaaa tcgaacgaat
    18001 atgccaacct ggtcggtggt gagcgttacg agccagatga agagaacccg atgctcggct
    18061 tccgtggcgc gggccgctat gtttccgaca gcttccgcga ctgcttcgcg ctggagtgtg
    18121 aagcagtgaa acgagtgcgc aacgacatgg ggctgaccaa cgttgagatc atgatcccgt
    18181 tcgtgcgtac cgtagatcag gcgaaagcgg tggttgaaga actggcgcgt caggggctga
    18241 aacgtggcga gaacgggctg aaaatcatca tgatgtgtga aattccgtcc aacgccttgc
    18301 tggcagagca gttcctcgaa tatttcgacg gcttctcaat tggctcaaac gatatgacac
    18361 agctggcgct cggtctggat cgtgactccg gcgtggtgtc tgaattgttc gatgagcgca
    18421 acgatgcggt gaaagcattg ctgtcgatgg ctatccgtgc cgcgaagaaa cagggcaaat
    18481 atgtcgggat ttgcggtcag ggtccgtccg accatgaaga ctttgccgca tggttgatgg
    18541 aagaggggat cgatagcctg tctctgaacc cggacaccgt ggtgcaaacc tggttaagcc
    18601 tggctgaact gaagaaataa aataaatccc cggcggcgtt cagtcgccgg ggttatgtga
    18661 tccccgaaga tgatacttat tcaatctctt cacagacatc ctgcgttaat cgccgcataa
    18721 tatcttttct taacaaaaac ttttgtattt tacctgaggc agtgcgcggt agtttttcga
    18781 ttaccacgat atgttcagga tatttatatt ttgcgacccg tttacggcta aaaaaagcca
    18841 ccacctcttc cagcgataat gaatgatgcg gcgctttcag cacgacataa gcgcatgatc
    18901 gttcacctaa acgttcatcg ggcattgcaa ccacacaggc atcgtgaatt ttaggatgct
    18961 gcaataaaat atcttccact tcacggctgc taatgttttc gccgccgcgg acaataatat
    19021 cttttttgcg tccggtaatt tttatatagc cagcctcatc catgcggcag agatcgccgc
    19081 tgtaatacca gccttcttcg tccagcgcac gggcggttaa ttcaggttca tcaaaatacc
    19141 ccataaacac attggggcca cgcgaggctt cttcaccttc gcaacctggc ggtaaggtct
    19201 tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc agcgtaacca tcggtgtgca
    19261 taaagcgcga caaaggatca tcgagattca ccaccgcatg cggcgaactt tctgtggaac
    19321 cataaacact taataattta atgccgcgct gctggcattc acgtgccact tttttgggga
    19381 ttgtggtacc gccgcaaaga aagaaacgca gcgctgaaag gtccgcgggt tgtttctcta
    19441 gtacattcaa aagatcatag acaaacggcg ttgcgcctag catacaggtg caacgctgct
    19501 gctcaagcag cgcgagacac gcatcaggag tgaaaatatc taacaacacg ctgcgagcgc
    19561 caattaagaa tggtgccgtt acgccatgca gaaagcccgt tgcgtgacca agtggcgcag
    19621 gcatcataaa gacatcctgc caggtcagat tcagtcgcgc gcaataagcc cgctcactgg
    19681 cgagaatatt gttatgcgtt agcatcacgc cctttggcag accctcggtt ccggaggtaa
    19741 acagcaccgc agctaattca tcgccgtggg tcgttatcgc cgtggtcagt gaggtattgt
    19801 cggcgataat ctgacttaat gagagggaag aggtggcggg agccagtttg tccacaccga
    19861 caatttgttg tagttgtgga agctgatttt gcagcggcag gattaaatct accggacgcg
    19921 tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg acacttattg agcacccaca
    19981 ccagttctgc ttcccgccag gaaggcaaca gcggcacgga aaccgcaccg gttttcaggc
    20041 aggcaagata gataacggta aattcacacc agcctggcag ttgaaatgcg atgcgatcgc
    20101 ctgattcaat acccttcgct aacatccagt ttgccagaca gctcgcggcg tgatcgagcg
    20161 cgctataggt gtacgatgct ccatgattat cgaccacggc aattttgtct ggcatcgcac
    20221 gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc ccatagccct tgctgacgat
    20281 acgccgcacg acgttgttcg ttgaacgtta atgtcacttt catgttggac tctcgaaaca
    20341 gaacatcagg cccgagatgc gggcctgtgg gatgcatgat tagccgtagc gaaaatcaat
    20401 accgaaggtg cctgcgggat attgccactg ttcgagaata gtgttaccgc acagcacccg
    20461 acaggtgccg cactccagac aaccggcaga atcaaaatga atgttgccgg cgtcatcctg
    20521 cttataaagt ccggcagggc aggctttcat taatttacgg aattcattga tatcgggatt
    20581 tgccgccaga atgatatgcg ggtggccctc atcaacatgg aatttattga cgcctaattt
    20641 gatgtcgacg ttaaccgtag cgttctggct catagcgcgg ttgctccctt aatgccatct
    20701 ttcagcaagt tgatcagccc aattttcttc gcatgtccca tgatcatttt gcgtaccggc
    20761 tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga tgtcggcgac cattcgtggg
    20821 tattggctaa acaggcgcgg gttttccatc agcgccggga tcttgcggaa atgctgcata
    20881 tcgcgcatga cgcagctttg ttccagctca cgtttgtatt gcgccagact gctggcggag
    20941 aaatcctcgc gttctttggc ggcgatcacc gttgtggcgg cagcctgagc cgatgcaatg
    21001 gctaactcca tgccacgaac tgtaaaaccc aaattcaggc agaagcctgc ggcgtcacca
    21061 acgatcatca cgccatcgtt aaccagctgc ggcaccattg ccagaccgcc ttctggcacc
    21121 atatgcgcgg aatattcaag cagtttgccg ccgctaatta gtgggcgaat ggcggggtgt
    21181 tgtttaaaat cttccagcat ttgcggcacg cttttttgcg catgggcgat atcacccaat
    21241 ccacaaacca gccccaacga tacggaatcg ttgttggtat agaggaatcc gccacccatc
    21301 aggccgtcag aaggggaacc ggcaaacagc caggcggcac cttcctcgcc cgtaatatta
    21361 aagcgatcgt tgatctgttc tggcgtgagg ccaataacct ctttaacacc aacagcgtaa
    21421 tgatgcggat cggaagcggg aaccattccc agcgagcggc caagcatcga gttaacgcca
    21481 tcagccagaa tcaccacatt cgcttcgaga atatcatccc cagcctgcac gccagttacc
    21541 ttgtttcctt cacgaaccag cgcatcgacg cgaacgcctg ggataaactg cgcgcccgcc
    21601 tgctcggctt gttccatcaa ccacgggtcc agacgattac gcaatacggt ataagatgcg
    21661 tgttgcggaa catctggctg ctcgcggtga aaatcgaggg taacggcgct ttcttcggtt
    21721 aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg gcgctgatgc tgcaaagcct
    21781 ggaatgattg cttcaagtgt gtgggcataa agacgcccgc cggtcatgtt tttacatccg
    21841 gcactgtcgc cgcgttctat caccaggaca tccagcccgg ctcgtgccat gaccagtgcg
    21901 gcaacgctac cagcaacgcc cgcaccgacc acaatggcat caaatttgtc atccgacatg
    21961 cgttatgctc ccttcccgct gcgtttacag cgggagatgt ggttgaaagg ggaattagcg
    22021 gctcaactgg ctaatcaggg cagggacgac tttgtagata tcgcccacca gaccgtagtc
    22081 ggcatagttg aagattggcg catttttatc tttattgatg gcgacaatca cttttgcgcc
    22141 gttgccgcca accatatgtt ggatctgccc ggagatcccc agcgtcaggt agagatcaga
    22201 tttcagcaac acgccggaga caccgatata acgttcacgc tccatccagt tctcgccttc
    22261 ggcaattgga cgtgaacagc cgacttcagc attcagcacc gccgccagtt cgtggaccat
    22321 tttcaggtca tcctgcgccg caagaccacg accgacgcca accacacgtt ttgctttgct
    22381 caggtccacg ctgcttgcgg ctttcgcacg gcgttcgcga cagagaattt catgacgcgg
    22441 agcaacataa ggtaccgttt ctgtcgggca ctgatgagag gtatcactgg tgcacggttc
    22501 ctgaacaccg ggtgcaaggg taatgatcgc cagcgggctg ttgatctttt cctgagcgaa
    22561 cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga ccatcgacaa tatccaccgc
    22621 cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt aaccgtgctg ccagagcttt
    22681 accacgtttc gtcgcggcca acagcaacat agcggggtgt ttatctttca gcagggcagc
    22741 aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg ttttgctcaa gaacataaat
    22801 acattttgga ccataaggca taactgcctg cgcctggtcg gtattttgta caatggcata
    22861 cacctgttgg ccccattgct gagcgccgcc aaacagttca gcataacgtt caggattatc
    22921 gctaaagacc cagacgctgt ttaattgact catcgtttct tctccgtagg cttcagttca
    22981 gggctttctt cagatgttcc gccagctcgg caatggcctc cggcgaatcg ttatcgataa
    23041 tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc gaccagttca gcaagtggcg
    23101 cgctctggct ccagtcaata tcacttgcct gccactgatt taccggtttt ttacccgcgc
    23161 cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat atcggaggtg acgcagagca
    23221 cggctggaac agagagttca ataacttcaa catcatcttc aagcgtgcgt tcaatcacca
    23281 gtgtattgcc ctgacgctga atagcactca ctgcattaat caccggaagt tgcagaattt
    23341 ctccgaccag caagccaacc tgctgggcat aaaggtcgcc ggaaccttca ccaaagatca
    23401 gtaaatcgaa gccgatcttt tcaattgctg ccgccagcgc ttttgcggta tcgagaggca
    23461 gtgcatgttc aagttgcgca tcctgcacca aatacaggct gtgcggcccg cgggatagca
    23521 cgtctttgcg cactttcgag ttctgcaaca atgagccgcc aacggtcagc gcggctatct
    23581 catcgtcatc tgttgtgagc tggcttgcag cttcaatggc attgagatcg aactggctga
    23641 ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg agtgacaaca atgtcctgtt
    23701 cttcaggcac cagcttaaag caggttatta ttttcatgat ttctcctgtg agtcgcatac
    23761 ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata gcggtgcggg
    23821 gttttcgacg gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa tgcgatcgct
    23881 aaaacaaaag tgcaatttgc ggcaatatag ttattccatg accgtacaat cttgcattca
    23941 gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa tacacgccgt
    24001 attttatccg gcatatattg tttttgcttg ctcatggaat agggaggttg tcatgtatca
    24061 acgctgtttt gataacgcca gtgaaacgct gtttgtcgcc ggtaaaacgc cacggctttc
    24121 acgttttgca tttagcgatg atccaaaatg ggagtccgga catcacgttc atgacaatga
    24181 aaccgagttg atttacgtca agaaaggggt tgcaaggttt accatcgatt cttcgttata
    24241 tgtcgcacat gcggatgaca ttgtggtgat agaacgcggc aggctgcatg cggtggcctc
    24301 tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc agtttcaggg
    24361 ggctgaggaa aatcagctac tgcaaccgca ttcttgtccg gtaattgccg cggggcaggg
    24421 aaaagaagtc ataaaaacct tatttaatga gctaagtgtg attttgccgc aaagtaaaaa
    24481 tagccaaaca tcttcgttat gggatgcatt tgcctatacg ttagccattc tttactacga
    24541 aaattttaaa aatgcttatc gttcggagca ggggtatatt aaaaaagatg ttctgataaa
    24601 agatattctt ttctatctga ataataatta tcgcgaaaaa atcactttag aacagttatc
    24661 gaaaaaattt cgtgccagcg tcagttatat ttgccatgaa tttaccaaag agtatcgtat
    24721 ttcccctatt aactatgtta ttcaacggcg tatgacggaa gcaaaatggt cactcactaa
    24781 tactgaatta tcacaggcag agatctcctg gcgtgtgggt tatgaaaatg tcgatcactt
    24841 tgccaaactg tttttgcgcc atgtcgattg ttcgcccagc gattaccgta gacaatttaa
    24901 aaactgtttt gcggaacaag aaatcctatc tgaatttcct caaccggtaa gtcttgttgg
    24961 ataaatctgg cggcgcagtt tgttaagaag ctgcgccgcc tgcagattat ttgttctgat
    25021 agtctttcag gatctgccga cctgctacgt aaatcataat ttcgtctgta ccgccgccga
    25081 tacgttcaca acggacatca cgccagaagc gggagacgcg cgcttcatcg gtatagccca
    25141 gaccgcccat gatttgaatc gcatcatcaa tgacttccat tgcggtacgt gcgcaataca
    25201 gttttgccag cgccgcgctg gtgcgcagtg actgatgctg atcggcttgc catgccactt
    25261 tcagcaccat gttgcgcatg ttgtcgatct taatcgccat cagcgccagt ttttcctgga
    25321 tcatctggtt atgaccaatg ggcttaccaa aagcgatacg ttggttggca tagcgggcgg
    25381 catcttcaaa ggcgcattcg gcaaagccgg tgctgcgcgc ggcgttgatc aggcgctcca
    25441 tctcaaagtt gtacatcaca ttgaggaaac ccattccttc ttcgcccacc atgtcgctct
    25501 cttcaacctc cacgttgtcg agatagactt cgcaggtgct gagcatatgc cagccgattt
    25561 tatgcagcgg gttaatctta atgccgggct tactggagtc gacccaccac agggtgaagg
    25621 cttttttggg atctttcggt tgcggatcgc gcgccaacac cagcatatac gggtactctt
    25681 tcgcaccggt aataaaggtt ttttgtccgt tgatgtagac cttgccgttt ttacgcgtgt
    25741 aagtggtagt ggcactgttg ttatctgagc cagcacctgg ttccgtcaac gccagggcat
    25801 aggcgggatc acccgtttcc agggtgcttt ctgccgtttt acgtagctgc tctgcagaac
    25861 cgaaacggcg catactgtga atacattgac cgttggtgat caaaaatgcc ggagcaccgc
    25921 attttgacac ttccatcagc gccagcattt gggtgacgta atccgcaggg ataccaccaa
    25981 attcttccgg cacgccaagc atggaaatac cgttatccgc cagcgcccgc ataaactcac
    26041 gcggatatgt cccgttttga tcgcaggtgc ggaaatactc ttccggaaag ttagtcgtaa
    26101 tcagttcgcg aatactggcc agcagcagtt cttgttcttc agttaaagaa aaatccatca
    26161 ttatactcct attaatgagc cgcttcaggc aggacaaaac ccatcgccgc atcgataaat
    26221 aatctttcgt ccatcagttt gagttctggc gaaatcactg gggtgaagtc cattttgtcg
    26281 agaatatctt tttgtaaatc gacgccaggg gcgatttcga ttaaatgcag gccgtcttct
    26341 ttcagcgtga atacagcacg ttcggtgatg taacgaacat ccagtccccg ttcgagcgcg
    26401 atttttccgc taaaagtaat ttccggtagt tcccgaataa atttatttac ccgtccttcc
    26461 tggacgatat gcaatttgcc atcggcaatt tctgttttta aactgcccgc agttaacgtg
    26521 ccgcagaaaa taattttctt cgaggtggca ctgatatcaa taaatccacc ggtacccatg
    26581 attttgccat tgaatttatg cacgccgacg ttaccgtgct ggtcgacttc agcaaaactc
    26641 aaataacaaa catccagacc accaccgtga taaaaatcaa actgggacgt catatccaga
    26701 atggcgcggg tattcacgtt cgcgccaaag gcgataccct gtgaagtaat tccgccaatc
    26761 ggacctgttt ctaccgtcag aataaagtca tcagcacaac cttcttcccg ggcgaccagg
    26821 ccaataccgt cagcaatacc gacgccgaca ttccccaccg cgcctttacg catttcgaat
    26881 aatgcacgcc gcgcaactaa tttacgttgg tttaggggca ggctaagttt ggggctgtca
    26941 tcaagggtga agtcaccaga aataaaacgg ttaaccggcg caccgccata cagttggctt
    27001 tgatccggat cgaccaccac aatatccacc agataacccg gaatacggac agatttagga
    27061 tgcagcgtgg ctttcttaac cattttctgc acctgcatca tcacaatacc gccgttattg
    27121 tgcaccgcct gggcaataac caatgcgtcg agatacatca cctcatcttc aaaagtggcg
    27181 tagccttcac tgtcgcaggt ggtagcgcga atgaaggcga tatctggcgc aatcggtttg
    27241 taatagagat attctttgtt atcaaactcg accagtttaa tcaggtcttc tttcgtgact
    27301 tcattcagtt tgccgccttg ctggcgtgga tcgacaaatg tcccgatgcc aatatcacta
    27361 ataataccag gctggtgggc tgcggcggcg cgtaaggttt gtgtaagtac accttgtgga
    27421 tagttataag caataatttt attttgttct gcgagttcag aaatacgcgg cgattgtccc
    27481 cagtgaccac ataatgccca tttcaccaga ccttcttgcg ccagaggact aataccacgg
    27541 tcggcgcgat cgccaagccc tgtcggacta ataatcgata aattacgtgg cgtttgagtc
    27601 tgtttatatt tatcagcaag agcagtaatt aacgtggtgg cttccagaat accgccgcca
    27661 gcgcctaaca cacaaagtgt tgcttcgtcg ggaatataat tcaccgcttc ctgtgccgac
    27721 aggaccggca ctcgtccatt aatacgaggt ggttttacag gtttcattgt taattcctga
    27781 ttcaggtgtg attttcccta cgccagatgg ataattagcg cacagagact cacgaaatat
    27841 ttttattaaa tacggctgaa tatatttttt tagttcggcg ggggaggata ttcccgccga
    27901 aatattattg cttatgcctg atgtaaaata gttaatactg tgcgcaaatc atttaccgag
    27961 atttgccctg gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact
    28021 tcaccagcca gacgagaaat tacgccagtt tttgccattg acatcgtgat gattggacga
    28081 tcggcatact gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta
    28141 ctttgcggca tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga
    28201 cgggcaatga tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact
    28261 ttcacatcat gcgcgtgggc gtaggcgacg gtttctttaa cctgatcatc gccggtaaat
    28321 aactccagat cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata
    28381 taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc
    28441 agcggttttt ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca
    28501 ttggagaggt cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga
    28561 taggcgagag cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt
    28621 ttaggtgcgc ccgtaccaat gacgagatct tttacagtta cggttttcat tttttaccct
    28681 ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga
    28741 gggaaatctt tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg
    28801 tatccatcaa tcgttttgca accagcttgt tgcgcctgct gcaataactt cgtcatatgc
    28861 gggttataca cgcattcagt gaccagcagt cccggatgta acagactgat atcattaact
    28921 aatgattcat tctcaagggg tttcataccc acttttgtgc cattggttaa aatgtcggcg
    28981 gaagccaggg cttcagcaaa ggcttgttga tcggcgagat cggtaaccgt gacgacacaa
    29041 tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga
    29101 cggttaaaga gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt
    29161 gaggcacctc cggcccctaa cagcaccatt gttttgcctt tgatatcaaa accgctctct
    29221 ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc cgcgcaggta gccatcatca
    29281 ttaacgatgg tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat
    29341 tcacacgcca gttgtttgtt cggcattgat acaccagttc cgcgcatttt tagagctttt
    29401 aatccttcaa ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat
    29461 ggcaatcccg ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata
    29521 ggataagcca tcaacccaat caattcgtat tttgcggtaa catccataat taactccttt
    29581 aacctctatg cttaattgtg tttagccggg tgctaaattt acgctcgcca aaccgcacat
    29641 cattctcagg aataatgaaa acgcggtaat agcggataaa gacaataatt gcggtaatca
    29701 gggccagcag cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca
    29761 gataaccggt aatcagcgga ataacaaaat tagccaatcc acccatcatc atataaatac
    29821 tggtgacttt ggctttgctt ttggggaaaa actctgacat gaccgaaacg ccgagctgta
    29881 aaatgccacc agctgctgag aaaccgataa caaaggctcc ggcattacac accagtggag
    29941 aagggtacag gtagataatg gctgctgtta ttgttgccag tgcagagtta aatacattag
    30001 cccagatggg ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg
    30061 agcccatact gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag
    30121 ccatcgcata tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac
    30181 cgaacagtac cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta
    30241 attcattagc tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca
    30301 gcgtgatcag tacaaatagg atgcctggaa taatcagccc atagccgtac cagatgttat
    30361 tgagcaacat atagctcacc agcattgggt agaacatttg tccgaatgac accatcgctt
    30421 taaccagtat gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc
    30481 cacccgtatc cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag
    30541 ttaaattcgg gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta
    30601 atatcaccgc ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca
    30661 atcgccccaa accaataccg gaaattaagt aggcaatgcc cgcgttgtca gtggaaaact
    30721 tttccgccag agatgacatg ttttgggcaa gcgtaataac actaatgccg tgcaggaagt
    30781 agctgaagta aatacaaaga acagccagga taaatggcgt gctgaaagcc ttattttgag
    30841 acattttcac agctccttgc tgaaagataa tttcgttacc gtacgtattg ttcttcgacg
    30901 gtttacataa ttactatact gaagatgatt tttcctgggt acatggcata aagtttatgt
    30961 ttccaggaat attgcatgtc atagtttgtt tttgatcgcg attttcctta gttagcgcag
    31021 aaagagttat ttatccgtaa gatcttgcat gacctgaaaa gaatattgca ttacccaccc
    31081 ggagcgacat tttcctttag cgagtgatgc cgcgtttttt tgcggctgcg taagccgata
    31141 aacagtgcca gtaaaaaacc gatggcagcg atggcggtat cgaaccacat aatatcggca
    31201 atacttcttt gggacagatg agccgtaatc aacggaatag taaaggtcgc aatactgccc
    31261 gcactgtaat agatccctgt agctttacct ttagcgtaag ggaaacgttc agccattaac
    31321 gtcaggccaa tttgcacaac acctccagca gaggtaaaac caatgacaaa agcaaatatt
    31381 atcaccacat aaaatgtggg atgcaggcag acggtaaaca gagcaataaa tgagataaag
    31441 gtgtacagca tcagtaatgt tgttgggcga acggtattac gaatgagtgg agcggtaata
    31501 aatacacaaa gcagcgaacc cacggtgtag atactgagta gtttaatcga catagtgtat
    31561 gacatgcctg caacaaattg tccgtactgt gccagccact ggctaaccag ataaaacgtt
    31621 gccattgaga tatagccata taaggtataa ctggctaaat cgataattga acagcgatgt
    31681 tccgtagagc tggtggtttt ctttatgaca ggtaagcgat gacccggatg gggtgggaac
    31741 gtacaaaggt ataaaaacag agcgttaata aacataatgc ctgcagcaat cataaaggac
    31801 caaccgaacc acagttcagc ccacactaac aggctaatga ttagcggtaa taaaaattgt
    31861 ccgctggaaa caaatgcttt aattaaaata ttggctgtcc caggtgagcg tggaaaagct
    31921 tccattaaac tgggataagt gcctgcatcg agaaaactgt ttgccattcc tgccagaaag
    31981 ccaaaaacat aagcgataat gatgttattg gtgtgcagga tgccaaaaaa gaaggccata
    32041 tagcagcaca tcccgagcat gataaaaggg cggcgaccaa agcgatcgga taataatcct
    32101 gcaaaaagca agacacttaa tcgaccaatg cccagcgatg agataactat cgagacaccc
    32161 gcggcattag tctgccaaag tgtctccagc gaggccatat tcaggctcat caaaatgacg
    32221 cccataccat gcaccaggta attaaaatac aacccaagtg cggtagggta atagggattt
    32281 ttcataggtt ctacttagca aaggtgcttt aaaaagtgta gcgatgttgt gtcagtatcc
    32341 gttcattatt gttgtaacaa cgacaattcc tttttgtgga gaaattaaca aagccgctcc
    32401 agatcgtgtg catataattc agcggctacc gattgataga tcttccagtc aataaaattg
    32461 ccatcagggt agacttgttg aaacgcggtt aagtcgggga aaaaacgcat ggtgttgttg
    32521 ccgatgcggt tatttatttt ctctatgatg gcatgaagat gtttttttcg ttgctgacgc
    32581 atagccaaca gttgctcgac tacacgatca ggaatggcgc atttgccatt ttcccactga
    32641 cgccagcttt cactgtcacc ggtctgggca atccaggtcg cgcattcatc aatagtcatg
    32701 gcaaaaatat ggcgaagtgc ctggagttca taagcgttca tcgtgtaatg gctgcatgaa
    32761 ataaacaata aaatataaat aacattatca gcctgctgac ggcttgaaga gagtcacagt
    32821 atcttgtgca acattattgg aaagagtgag gggcgaggta gcgaagcgga aaaatgttaa
    32881 aaaaaagccc atcgtgggag atgggcaaag actacacaca gcaattcgtt gtttcactca
    32941 ggggatttcc atgcttataa atcaatatgt tgatttataa ccgtaagcta atagtaggca
    33001 tacggacttt ttgcgtcgat cagattcgtc tcattagttt gaaaactgta aagaatttgc
    33061 gacaggtact accagctgaa tggcggtaag caggaaaata tggggaagag caagcaaata
    33121 tcaattagcg ggggttaaca atcaggcggc agagccgcct gatgagaaat tacttattcg
    33181 gtttctcgat ttcgccaagt tcttcgagaa tttcttccgg ttggtccgtc ggcggcggga
    33241 cttcttccac ccacgcggca aacagacgcc aggaaacggc taacagaacc ggaccaataa
    33301 acagaccgat catcccgaaa gcaatcaaac caccaataac accagagagg atcaggatca
    33361 gcggtaaatc ggcacccatg cgaattaaca ttgggcggat gacgttatcc agcgtgccaa
    33421 ccacaccgct ccacactaac aataccgttc cccaggtggt atcgccagtc cagtagagcc
    33481 agataatcgc cggaatcagt accggcaacg ggccaaactg gacaaggcag gagaggatca
    33541 ttaacaccgt tagcaacgtt gcataaggta cgccggatac ggcgaggccg ataccgccaa
    33601 gcactgcctg tactaacgcc gtcaccacca cacccagcgc caccgcgcgg atagcctgtg
    33661 ccgccagcag tacggcggca tctccgcgaa cgcctgccag acgggttgca aaatggcgaa
    33721 tgccttgtgc cacctgttca ccgcgccagt acagcagggc actgaagaga agcatcagcg
    33781 cacaatgcac cataaagcgc ccgatatgcg ccgcctgccc aacgaaccag gtggtggtgg
    33841 tgccaatata agggcggact ttcgccatga tcgccgtccc ccccatatcc agcaagttgt
    33901 gccagcctgc atacagcttc gcgccaatca ccggaatggt attaagccac gccagatcgg
    33961 gtaacgtcat gtcgccgctg gaaatggctt taattagcgg gccgctgccg tcgacgatac
    34021 tgttaaccag caaagcaata gggatgataa acaccatcac taataacagc gtcatcacca
    34081 gaacggcgag agagcggcgg ccaaacatga tcttttgcaa acgtaacaat accggccagg
    34141 tggcgataac caccgtaccg gcccatgcaa agccgagaat aaagggttga acaatccaca
    34201 gacatgccac aatcatgatg gctaaaaaca gcaccgaaag cagaatttgt gcgacatccc
    34261 tgggctgacg aacatttacc ataactactt ttcacctttt ttgtgcgtca aatcgtcgac
    34321 gcgacgtgaa cacgcgaaac tcacctgcat aatgatgagc aatttcagcg gtttttaaca
    34381 ggccgattct gcccttaatt ctgatgggcg tgttagaaaa aaatgtggta caacaactca
    34441 ggcaacacgc aaacgattta ctcgtcgtat ttcaagttgc atgtgctgcg gctacgttcg
    34501 ttcaccccag tcacttacta ttgtaagctc ctggagttca ctcgcttgcc gccttcctgc
    34561 aactcgaaat ccatagagta aataaacatc acctttcagt acgctattgc agccgcctga
    34621 aagaatagtg tatcgaccta catcacagac cgcaggaaag ggtcaatata atgattccac
    34681 agatttccca ggcacccggc gtcgttcaac tggtgcttaa ttttttgcaa gagctggagc
    34741 aacaaggttt taccggcgat acggcgacaa gttatgccga tcgtctgaca atgtcgaccg
    34801 acaacagtat ttaccaactt ctccccgatg cggtggtatt tccgcgttca accgcagatg
    34861 tggcgctgat cgcccgtctt gccgcgcagg aacgctattc atcgctgatc tttacccccc
    34921 gcggcggcgg caccggcacc aacggtcagg cgctcaacca ggggattatt gttgatatgt
    34981 cccgccatat gaaccgcatc atcgaaatta accctgaaga gggctgggtg cgcgttgagg
    35041 ccggggtgat aaaagatcaa ctcaatcagt acctgaaacc gttcggctac ttttttgcgc
    35101 cggaactttc gaccagcaac cgggcaacgc tcggtgggat gatcaatacc gatgcttctg
    35161 gtcagggatc gctggtctat ggcaaaacgt cggatcacgt acttggcgta cgtgcggtgt
    35221 tgttgggggg cgatattctc gatacgcaac ctttacccgt cgaactggcg gaaacgctgg
    35281 gtaaatccaa taccacaatc gggcgaattt ataacacggt ttatcaacgt tgccgtcagc
    35341 aacgccagtt aatcatcgac aacttcccca aacttaaccg ctttcttacc ggttacgatc
    35401 tgcgtcatgt ctttaacgat gagatgaccg agttcgacct gacgcgcatt ctgacgggtt
    35461 cagaagggac gctggccttt attaccgaag cgcggctgga tattacgccc ttgcctaaag
    35521 tgcgccgtct ggtgaacgtc aaatatgact cttttgactc cgcgctgcgt aacgcgccgt
    35581 ttatggttga ggcgcgggcg ctttcggtag agacggtgga ctcaaaagtg ctgaatctgg
    35641 cgcgggaaga tattgtctgg cattccgtca gcgagttgat taccgatgtg cctgacaaag
    35701 agatgctcgg gctgaacatt gtggagtttg ctggtgatga tgaggcgctg attgacgagc
    35761 gagttaatgc tctctgtgtg cggcttgatg agctgattgc cagccaacag gcaggtgtga
    35821 ttggctggca ggtgtgccgc gagctggcgg gtgttgaacg tatctatgcg atgcgtaaaa
    35881 aagccgttgg tctgcttggc aatgccaaag gtgccgctaa gccaattccg tttgctgagg
    35941 atacctgcgt accgccggaa catctggccg attatattgc tgaatttcgc gcgctgctcg
    36001 acagccacgg cttaagctac ggtatgttcg gtcacgtcga cgcaggtgtc ttgcacgtcc
    36061 gtccagcgct ggatatgtgc gatcctcaac aagagatttt gatgaagcaa atctctgatg
    36121 acgtagtggc gctgactgcg aaatacggtg gtttgttgtg gggcgagcac ggcaaaggtt
    36181 ttcgcgctga atacagcccg gcgtttttcg gtgaggaact ttttgcagaa ctgcgcaaag
    36241 tgaaagcggc atttgacccg cataaccgac tcaacccagg gaagatttgc ccgccagaag
    36301 gtctcgatgc gccgatgatg aaagtggacg cggtgaagcg cggtacattc gatcggcaga
    36361 tccccattgc ggtacgccag cagtggcgcg gtgcgatgga gtgtaacggc aacggtttat
    36421 gcttcaactt tgatgcccgt agtccgatgt gtccgtcgat gaagatcacc cagaaccgga
    36481 ttcattcacc gaaagggcgc gcaacgctgg tgcgtgaatg gctgcgtttg ttggcggatc
    36541 gcggcgttga tccactcaaa ctggaacaag aactgcctga atccggcgtc agtttgcgga
    36601 cgttaattgc ccgcacgcgc aatagctggc atgcgaataa aggcgaatat gacttctcac
    36661 acgaagtcaa agaggcgatg tcgggctgtc tggcctgtaa agcgtgttcg acccagtgcc
    36721 ccatcaaaat tgatgtgccg gagtttcgct ctcgttttct gcagctctat cacacccgtt
    36781 atttacgccc gctgcgcgac cacctcgtcg ctacggtcga gagctacgcg ccgctgatgg
    36841 cacgcgcgcc gaaaaccttt aacttcttca ttaaccagcc gctggtgcgc aaactctcgg
    36901 aaaaacatat cggcatggtt gatttgccgc tgctgtcggt cccctcgcta caacaacaaa
    36961 tggtggggca tcgctcggca aacatgacgc tggaacagct tgaagctctc aatgcagagc
    37021 agaaagcgcg cacagtgttg gtggtgcagg acccctttac cagctattac gatgcgcaag
    37081 tggtggcgga ttttgtccgt ctggtcgaaa aattaggttt ccagcctgtg ttactgccat
    37141 tttcgccaaa tggcaaagcc cagcatatta aaggttttct taatcgtttt gcgaagacgg
    37201 cgaaaaagac ggcggatttc ctcaaccgta tggcgaagct gggtatgcca atggtgggcg
    37261 tcgatccggc gctggtactt tgttatcgcg atgaatataa actggcgctg ggcgaggaac
    37321 gtggcgagtt taacgtctta ctggcgaatg aatggctggc aagcgcactt gagtcacagc
    37381 cagtggctac agtcagcggt gaatcatggt atttctttgg tcactgtacc gaagttaccg
    37441 ccttgccggg tgcgccagca caatgggccg cgatatttgc ccgttttggc gcgaaactgg
    37501 aaaatgtcag cgtgggttgc tgcggcatgg cagggactta cggacatgaa gcgaaaaacc
    37561 ataaaaattc gctcgggatc tatgagttat cctggcatca ggctatgcag cgactgccgc
    37621 gtaaccgctg tctggcgacc ggatattcct gccgtagcca ggtaaaacgg gttgaaggca
    37681 cgggggtacg ccatcctgtg caggctttac tggagattat taaatgatat ggaaacggaa
    37741 aatcaccctg gaagcactga atgctatggg tgaaggaaac atggtggggt tcctggatat
    37801 tcgctttgaa catattggtg atgacaccct tgaagcgaca atgccagtag actcgcggac
    37861 aaagcagcct ttcgggttgc tgcatggagg agcatccgtg gtactggccg aaagtatcgg
    37921 ttccgttgcc ggttatttat gtaccgaagg tgagcaaaaa gtggttggtc tggaaatcaa
    37981 tgctaaccac gtccgctcgg cacgagaagg gcgggtgcgc ggggtatgca aaccgttgca
    38041 tctcggttcg cgtcaccagg tctggcagat tgaaatcttc gatgagaaag ggcgtttgtg
    38101 ctgttcgtca cgattgacga ccgccatttt gtgacgttgt caccagaaaa gtgtgacgca
    38161 tattgccttt ggtcattatc tgcacagtaa acggtggtat actgtgcagg tcttgtctaa
    38221 aagcgaggat gctatgtcta ctcaacttga tcccacccag ctggccattg aatttttacg
    38281 ccgtgaccag agcaatcttt cacctgccca atatctcaaa cgtctgaaac aactagaact
    38341 tgaatttgct gacctgctta ctctctcttc tgctgaatta aaagaagaaa tctactttgc
    38401 ctggcgtctg ggcgtgcatt gatcctgact taatgccgca agttcgccaa atcgacctgg
    38461 attaacctcc gtccaggttc gatgaccttc ttaatactgc ttatctaccg cctgatattg
    38521 ttcaaaaata atactaatcg cagtttgtgt taaaacggcg ggttagcttt atgagattat
    38581 tcttatcgcc ccttcaagag ctaagccact gagagtgccg gagataagcg ccggatgggg
    38641 tagaaaccct taagcctgtg ttgcacagac ttaagggttt tcttatttct gaacgctgca
    38701 cttttttacc tatctcttaa atagtcattt tcaatacaac tttttctttt tcatcacagc
    38761 cttaatgctc cttagataat aaccattatc taacaatgag atacctaatt cttagcgtgc
    38821 ctgttaaccc acacatcagg gtctatgctt attaaaagca ggcaaaaagc atgttttacg
    38881 gtaaagcccc tgcgtttgct gggttgtact gataatcatt atcactaaca tgctgttata
    38941 cgctgaaagc gatgaagtga ggtaaatcga tggacatgca ttcaggaacc tttaacccac
    39001 aagatttcgc ctggcaaggc ttaacgctga cacccgcagc ggcgatacac atccgtgagc
    39061 tggtggcaaa gcagccgggt atggtcggcg tgcgcttagg cgtgaagcaa acgggctgcg
    39121 cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat ctgctgtttg
    39181 aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat gccgtttatt gatggcacgg
    39241 aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac cctaaagccc
    39301 agaatgaatg tggctgtggc gaaagctttg gggtataggc ggtactatgt ctcgtaatac
    39361 tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata aagaaggatt
    39421 cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg tggtgcgcgc
    39481 aatttcggcg aagcgtaatg agccggagtg gatgctgaag tttcgtctaa acgcctatcg
    39541 cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca agctgaatta
    39601 tcaggattac agctactact cagcaccatc gtgcggtaat tgtgacgaca cttgcgcgtc
    39661 tgaacctggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag aggtggaggc
    39721 ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat
    39781 tttcgactca gtttcggttg ccactactta tcgcgaaaaa ctggcggagc agggaattat
    39841 tttctgttcc tttggtgagg cgatccacga tcacccggaa ctggtgcgta aatatctcgg
    39901 caccgtggtg ccggggaatg acaacttctt tgccgcgctt aatgcggcgg tagcctctga
    39961 cggtacgttt atttatgtgc ctaaaggcgt gcgctgcccg atggaacttt ccacctattt
    40021 tcgcattaac gcggaaaaaa ccgggcagtt tgagcgcacc attctggtgg ccgacgaaga
    40081 cagctacgtc agctacattg aaggctgttc cgctccggtg cgtgacagct atcagttaca
    40141 cgcggcagtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt ccacggtaca
    40201 aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca ccaagcgtgc
    40261 tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg ggtccgcgat
    40321 tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg agttttactc
    40381 agtggcgctg accagcggtc atcagcaagc ggataccggc accaagatga tccacatcgg
    40441 taaaaacacc aaatcgacca ttatctcgaa agggatctct gccggacata gtcagaacag
    40501 ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg
    40561 cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg
    40621 caataacagc gcacaactgg agcacgaggc aacgacatca cgtattggtg aagatcaact
    40681 gttttactgc ctgcaacgcg ggatcagcga agaagacgcc atctcgatga ttgttaacgg
    40741 tttctgcaaa gacgtgttct cagaactgcc gctggaattt gccgttgaag cacaaaaact
    40801 cctcgccatc agtcttgaac acagcgtcgg ataaggaata aacatgttaa gtattaaaga
    40861 tttacacgtc agcgtggaag ataaagctat cctgcgtgga ttaagcctcg ccgttcgtcc
    40921 cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct tatcggcaac
    40981 gcttgccggg cgcgaagatt atgaagtgac gggcggcacg gttgagttca aaggcaaaga
    41041 tttgcttgca ctgtcgccgg aagatcgtgc aggcgaaggc atctatatgg ccttccagta
    41101 tccggtggag attccaggcg tcagtaacca gtttttcctg caaacggcac ttaatgcagt
    41161 gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt tgatggaaga
    41221 gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa acgttggttt
    41281 ttccggcggc gagaaaaagc gcaacgatat tttgcaaatg gcggtgctgg aaccggagtt
    41341 atgcattctt gatgagtcgg actccgggct ggatattgac gcattaaaag tggtcgccga
    41401 tggcgtgaac tcgctgcgtg atggcaagcg ctcattcatc attgttacgc actaccaacg
    41461 cattctcgac tacatcaagc ctgattacgt ccatgtgcta tatcagggac gaattgtgaa
    41521 atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct ggcttaccga
    41581 acagcagtaa cgcgctgcaa cagtggcatc acttgtttga agctgaaggg acaaaacgct
    41641 caccgcaagc acagcagcat ttacaacaat tgctgcgtac cggactgccg acacgtaaac
    41701 atgaaaactg gaaatatacg ccgctggaag ggctgaccaa tagccagttt gtcagcattg
    41761 cgggagagat atccccacag cagcgagatg ccttagcgtt aacgttagac gctgtgcggc
    41821 tggtatttgt cgatggacgt tacgtgtcag cactgagcga tgcgactgaa ggcagcgggt
    41881 atgaagtgag cattaacgac gaccgtcagg gggtacccga cgctattcag gcggaagtgt
    41941 ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgtg aagcgcggtc
    42001 aacggccggc aaagccattg ctgttaatgc atattaccca gggcgtggca ggtgaagagg
    42061 tcaacactgc ccattaccga catcatctgg atctggcgga aggtgccgaa gcaacggtga
    42121 tcgaacattt tgtcagcctt aatgatgctc gtcacttcac cggcgcacgg ttcactatca
    42181 atgtcgcagc gaacgcccac ttgcagcata tcaagctggc gtttgaaaac ccggtcagtc
    42241 accactttgc ccataacgat ttgttgctgg ctgacgatgc caccgcattt agccatagtt
    42301 tcctgctggg tggcgcagtg ttacgacaca acaccagtac gcaactcaat ggcgaaaaca
    42361 gcacgctgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt gatacccgta
    42421 cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa actatcgtca
    42481 gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt cgcgcagcac gccatcaaaa
    42541 cggatggtca gatgaccaac aataatctgc tgatgggcaa actggcagaa gtggatacga
    42601 aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg acggtggggc
    42661 gtattgatga tgaacagatg ttctatctgc gctcgcgcgg gatcaatcag caggatgccc
    42721 agcagatgat catttacgcc tttgctgccg aactgacgga agcactgcgt gatgaggggc
    42781 ttaaacagca ggtgctggcc cgaatcggtc aacgactgcc aggaggtgca agatgacttt
    42841 ttccgtcgac aaagtgcggg ccgactttcc ggtgctttcg cgtgaggtaa acggtttgcc
    42901 gctggcttat ctcgacagcg ccgccagtgc gcagaaacca agccaggtga ttgactccga
    42961 agccgagttt tatcgtcacg gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc
    43021 ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta ttaatgcccg
    43081 ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa
    43141 tagttggggc aacagcaacg tgcgggcggg cgataacatc atcatcagtc agatggagca
    43201 ccacgctaac attgttccct ggcagatgct ttgcgcacgc gttggcgcag agctgcgtgt
    43261 gatcccgctc aatcccgacg gtacgctgca actggagacg ctacctaatc tgttcgatga
    43321 gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg cttggcacag aaaatcccct
    43381 ggcggaaatg atcacgcttg cgcatcagca tggcgcaaaa gtgctggtgg atggcgctca
    43441 ggcggtgatg catcatccgg tggacgttca ggcgctggat tgcgattttt acgtattctc
    43501 cgggcataaa ctgtatggcc ccaccggaat tggcattctt tatgtgaaag aagccttgtt
    43561 gcaggagatg ccgccgtggg aagggggcgg ttctatgatc gccaccgtca gcctgagtga
    43621 aggcactacc tggaccaaag caccatggcg gtttgaagcc ggtacaccca ataccggggg
    43681 catcattggt cttggcgcgg cgctggagta tgtttcggcg ctggggctta ataacatagc
    43741 cgagtatgaa cagaatctga tgcactacgc gctatcacag ctggaatctg taccggatct
    43801 cactctctat ggcccgcgga acaggcttgg cgttattgct tttaatcttg gtaaacacca
    43861 cgcctatgat gttggcagtt ttctcgataa ctacggcatt gctgtgcgta ccggacatca
    43921 ctgcgctatg ccattaatgg cctattacaa cgtccctgct atgtgtcggg cgtcgctggc
    43981 catgtataac acccatgaag aagtggatcg tctggtgacc ggactgcaac gtattcaccg
    44041 tctgctggga taacagggag gcactatggc tttattgccg gataaagaaa aattgctgcg
    44101 taatttttta cgctgcgcca actgggaaga gaaatatctc tacattattg agctgggcca
    44161 gcgtctgcca gaattacgcg ccgaagaccg aagcccacaa aatagcattc agggctgtca
    44221 gagtcaggtg tggattgtca tgcgccagaa tgcgcaggga attattgaat tgcagggcga
    44281 cagcgatgcg gcgattgtga aagggcttat tgcggtcgtc tttattctct acgatcagat
    44341 gacgccgcag gatattgtca atttcgatgt gcgtccgtgg tttgaaaaaa tggcgctcac
    44401 ccaacatctc accccatctc gttcacaagg tctggaagcg atgattcgcg caattcgcgc
    44461 caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg ctcacattgc
    44521 cggatggtca atgtttacac tgacctgcac aggagtgttt tggtttcagg tgaacataag
    44581 cgccatccga cgtttcagcg tgagtctccg gcaaatacag gaggtttaca atgaaacgcg
    44641 cgtctttgct tacactcacg cttatcggcg cttttagcgc catccaggct gcctgggcgg
    44701 ttgattatcc gctaccacca accggaagcc gactggttgg gcaaaatcaa acgtatacgg
    44761 tgcaagaagg ggataaaaac cttcaggcta ttgcccgacg ttttgatact gcggcaatgt
    44821 tgatccttga agccaataac actatcgccc cggtgccaaa acctggtacg acgataacta
    44881 ttccttcaca actgttatta cctgatgcac cgcgtcaggg gattatcgtt aaccttgcag
    44941 agctgcgcct ttattattat ccgccgggag aaaatattgt gcaggtttat ccaataggta
    45001 ttggattgca ggggctggaa acgccggtga tggaaacgcg tgttgggcag aaaatcccta
    45061 acccaacctg gacgcctacg gcaggcattc gtcaacgttc gctggggcgt ggcattaaat
    45121 taccgccagt cgttcctgcc ggaccaaata acccgctagg acgttacgca ctgcgcctcg
    45181 cgcatggtaa tggcgaatac ctcattcatg gtaccagtgc gccggacagc gtcggtttgc
    45241 gcgtcagttc agggtgtatt cgcatgaatg caccggatat taaagccttg ttctccagcg
    45301 tgcggacggg aacgccggtg aaagtgatca acgaaccggt gaaatattcc gtggaaccta
    45361 acgggatgcg ttatgttgaa gtacatcgac cactatcagc agaagaacag caaaacgttc
    45421 agacaatgcc atacacactg ccagcaggct ttacgcaatt taaagacaat aaggctgtag
    45481 atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg gtatccggtt tcggtgagca
    45541 gtggagcaac tcccgcagcc agcaatgcgc cttcagtaga gtcagcgcag aatggtgaac
    45601 cagagcaagg gaatatgtta cgcgcgacgc agtagcggta aacgggagac aaaaaaaatg
    45661 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    45721 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    45781 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    45841 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    45901 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    45961 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    46021 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    46081 aaaaaaattg agagaaaaca ggatgcttcc at
//
LOCUS       DAPHJE010000023        44794 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPHJE010000023 DAPHJE010000000
VERSION     DAPHJE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.023"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            291..2996
                     /gene="malT"
                     /locus_tag="RH222_001308"
     CDS             291..2996
                     /gene="malT"
                     /locus_tag="RH222_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV3796360.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(3039..4055)
                     /gene="rtcA"
                     /locus_tag="RH222_001309"
     CDS             complement(3039..4055)
                     /gene="rtcA"
                     /locus_tag="RH222_001309"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV3796361.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4059..5285)
                     /gene="rtcB"
                     /locus_tag="RH222_001310"
     CDS             complement(4059..5285)
                     /gene="rtcB"
                     /locus_tag="RH222_001310"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV3796362.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETFPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5474..7072
                     /gene="rtcR"
                     /locus_tag="RH222_001311"
     CDS             5474..7072
                     /gene="rtcR"
                     /locus_tag="RH222_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV3796363.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRTPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(7054..7812)
                     /locus_tag="RH222_001312"
     CDS             complement(7054..7812)
                     /locus_tag="RH222_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV3796364.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7829..8659)
                     /gene="glpG"
                     /locus_tag="RH222_001313"
     CDS             complement(7829..8659)
                     /gene="glpG"
                     /locus_tag="RH222_001313"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV3796365.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8704..9030)
                     /gene="glpE"
                     /locus_tag="RH222_001314"
     CDS             complement(8704..9030)
                     /gene="glpE"
                     /locus_tag="RH222_001314"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV3796366.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9220..10725
                     /gene="glpD"
                     /locus_tag="RH222_001315"
     CDS             9220..10725
                     /gene="glpD"
                     /locus_tag="RH222_001315"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV3796367.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10779..11384)
                     /locus_tag="RH222_001316"
     CDS             complement(10779..11384)
                     /locus_tag="RH222_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796368.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(11387..12154)
                     /locus_tag="RH222_001317"
     CDS             complement(11387..12154)
                     /locus_tag="RH222_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796369.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(12157..12867)
                     /locus_tag="RH222_001318"
     CDS             complement(12157..12867)
                     /locus_tag="RH222_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796370.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(12882..14390)
                     /locus_tag="RH222_001319"
     CDS             complement(12882..14390)
                     /locus_tag="RH222_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV3796371.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(14515..16962)
                     /gene="glgP"
                     /locus_tag="RH222_001320"
     CDS             complement(14515..16962)
                     /gene="glgP"
                     /locus_tag="RH222_001320"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV3796372.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(16981..18414)
                     /gene="glgA"
                     /locus_tag="RH222_001321"
     CDS             complement(16981..18414)
                     /gene="glgA"
                     /locus_tag="RH222_001321"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV3796373.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(18414..19709)
                     /gene="glgC"
                     /locus_tag="RH222_001322"
     CDS             complement(18414..19709)
                     /gene="glgC"
                     /locus_tag="RH222_001322"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV3796374.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(19727..21700)
                     /gene="glgX"
                     /locus_tag="RH222_001323"
     CDS             complement(19727..21700)
                     /gene="glgX"
                     /locus_tag="RH222_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV3796375.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(21697..23883)
                     /gene="glgB"
                     /locus_tag="RH222_001324"
     CDS             complement(21697..23883)
                     /gene="glgB"
                     /locus_tag="RH222_001324"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV3796376.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24156..25259)
                     /gene="asd"
                     /locus_tag="RH222_001325"
     CDS             complement(24156..25259)
                     /gene="asd"
                     /locus_tag="RH222_001325"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV3796377.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            25452..26045
                     /gene="yhgN"
                     /locus_tag="RH222_001326"
     CDS             25452..26045
                     /gene="yhgN"
                     /locus_tag="RH222_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV3796378.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26102..27442)
                     /gene="gntU"
                     /locus_tag="RH222_001327"
     CDS             complement(26102..27442)
                     /gene="gntU"
                     /locus_tag="RH222_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV3796379.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     FAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(27446..27973)
                     /gene="gntK"
                     /locus_tag="RH222_001328"
     CDS             complement(27446..27973)
                     /gene="gntK"
                     /locus_tag="RH222_001328"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV3796380.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(28112..29107)
                     /gene="gntR"
                     /locus_tag="RH222_001329"
     CDS             complement(28112..29107)
                     /gene="gntR"
                     /locus_tag="RH222_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV3796381.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(29331..30026)
                     /gene="yhhW"
                     /locus_tag="RH222_001330"
     CDS             complement(29331..30026)
                     /gene="yhhW"
                     /locus_tag="RH222_001330"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV3796382.1"
                     /translation="MIYFRKANERGHANHGWLNSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(30149..31186)
                     /locus_tag="RH222_001331"
     CDS             complement(30149..31186)
                     /locus_tag="RH222_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3796383.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHLTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEQGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            31519..32007
                     /gene="yhhY"
                     /locus_tag="RH222_001332"
     CDS             31519..32007
                     /gene="yhhY"
                     /locus_tag="RH222_001332"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3796384.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     MK"
     gene            complement(32068..33810)
                     /gene="ggt"
                     /locus_tag="RH222_001333"
     CDS             complement(32068..33810)
                     /gene="ggt"
                     /locus_tag="RH222_001333"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV3796385.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            33930..34370
                     /locus_tag="RH222_001334"
     CDS             33930..34370
                     /locus_tag="RH222_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV3796386.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(34357..35100)
                     /gene="ugpQ"
                     /locus_tag="RH222_001335"
     CDS             complement(34357..35100)
                     /gene="ugpQ"
                     /locus_tag="RH222_001335"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV3796387.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(35097..36167)
                     /gene="ugpC"
                     /locus_tag="RH222_001336"
     CDS             complement(35097..36167)
                     /gene="ugpC"
                     /locus_tag="RH222_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV3796388.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(36169..37014)
                     /gene="ugpE"
                     /locus_tag="RH222_001337"
     CDS             complement(36169..37014)
                     /gene="ugpE"
                     /locus_tag="RH222_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV3796389.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(37011..37898)
                     /gene="ugpA"
                     /locus_tag="RH222_001338"
     CDS             complement(37011..37898)
                     /gene="ugpA"
                     /locus_tag="RH222_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV3796390.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(37996..39312)
                     /gene="ugpB"
                     /locus_tag="RH222_001339"
     CDS             complement(37996..39312)
                     /gene="ugpB"
                     /locus_tag="RH222_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV3796391.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(39708..40421)
                     /gene="livF"
                     /locus_tag="RH222_001340"
     CDS             complement(39708..40421)
                     /gene="livF"
                     /locus_tag="RH222_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV3796392.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(40423..41190)
                     /gene="livG"
                     /locus_tag="RH222_001341"
     CDS             complement(40423..41190)
                     /gene="livG"
                     /locus_tag="RH222_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV3796393.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(41187..42464)
                     /gene="livM"
                     /locus_tag="RH222_001342"
     CDS             complement(41187..42464)
                     /gene="livM"
                     /locus_tag="RH222_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV3796394.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(42461..43387)
                     /gene="livH"
                     /locus_tag="RH222_001343"
     CDS             complement(42461..43387)
                     /gene="livH"
                     /locus_tag="RH222_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV3796395.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(43435..44544)
                     /gene="livK"
                     /locus_tag="RH222_001344"
     CDS             complement(43435..44544)
                     /gene="livK"
                     /locus_tag="RH222_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV3796396.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT        10924 a        12324 c        11252 g        10294 t
ORIGIN      
        1 atagatttat taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc
       61 ttgcttaaat gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt
      121 tttaggtgag ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa
      181 aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga
      241 agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc
      301 cgtcaaaact aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg
      361 ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacgagtcct gcgggctacg
      421 gaaagaccac cctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc
      481 tggatgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc gccgtgcagc
      541 aggcaaccaa cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca
      601 gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt
      661 atctggtcat cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct
      721 tctttattcg ccatcaacca gaaaatctca ccctggtggt gttgtcacgc aaccttccgc
      781 aactgggcat tgccaatctg cgtgttcgtg accaactgct ggaaattggc agtcagcaac
      841 tggcatttac ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg
      901 aagccgcaga aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg gcactacagc
      961 taatcgccct ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg
     1021 cgggaatcaa tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg
     1081 atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgccc
     1141 tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg tctcgaagag attgagcgtc
     1201 aggggctgtt tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt
     1261 ttggcaactt cctgcgccag cgctgccagt gggaactggc ggcggagctg ccggaaatcc
     1321 accgtgccgc cgcagaaagc tggatggccc agggatttcc cagcgaagcg attcatcatg
     1381 cgctggcggc aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt
     1441 tgttcaacca tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca
     1501 gtttgctgga aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa agccaacatc
     1561 gctatggcga agttaacacc ctgctagccc gtgctgaaca tgaaatcaag gacatcagag
     1621 aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta
     1681 atccggatga agcggaacgg ctggcaaaac tggcactgga agagctgccg ccgggctggt
     1741 tctatagccg cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggcgaat
     1801 tgacccgctc actggcgcta atgcagcaaa ccgaacagat ggcacgccag cacgacgtct
     1861 ggcactacgc cttatggagt ttaatccagc aaagtgaaat tctgtttgcc caagggttcc
     1921 tgcaaaccgc gtgggaaaca caggaaaaag cgttccagct gatcaacgag cagcatctgg
     1981 aacagctgcc gatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg
     2041 cacggctgga tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg tcgtcttatc
     2101 agccacagca acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg
     2161 atttagataa cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg aatggcaaat
     2221 atcacagcga ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg
     2281 gcgataaagc cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca
     2341 accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt
     2401 ttgaacctgc ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga
     2461 tgagcgatct caaccgtaac ctgttgctgc ttaatcaact gtactggcag gccggacgta
     2521 aaagtgacgc ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta
     2581 tcagccattt tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac
     2641 tcaatacgct gccggaactg gaacagcatc gcgcacagcg tattctgcga gaaatcaatc
     2701 aacatcatcg gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc
     2761 atccggaagt gccagaactg atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc
     2821 tggggctgat ttactccggt tacagcaatg aacaaattgc gggcgaactg gaagtggcgg
     2881 caaccaccat caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc
     2941 aggctgccgt gcagcacgcg cagaaactgc tgaagatgat gggttatggg gtgtgagttt
     3001 agccggataa tgcattggat ccggtttaca tctttacatc attcaatact cacccgcgtt
     3061 acgccatctg tttctatcaa accaaaccgc accggcaaga aacgctccac caccgcgata
     3121 ttggtcagca gatggcatga aggatgggcg accttaaatt ctcccgcgcc cgccagcgcc
     3181 atcggcagta ccagttggtc ggcgagatat tcccccaccg ccgctgggct tgccaggtag
     3241 cgtttcacct ctttcactaa ctgcgcggct accacctcgg cactgacgcg cttttcaccg
     3301 acgacgaaaa agcgttcagt gatattttca ctttcgactt ccagcgagac ggtattaccc
     3361 ggcccctggt cgcgcggcag gttatgaata ttctgttcat gcagggaaaa actccccgcc
     3421 agtgtagcga tttcacgctc agcaacatgg cgcggcacac cagctaatag aacttctcca
     3481 cgcatctgca caatgttccc gcgctcgcca agttgcaagg tgttaaacga tgccacgggc
     3541 gagacttccg ttgccaccac gccgcctccg gcaggataaa aaccgtgacg taacagcgtg
     3601 gtttgctgat gaattcctat tttcgccagc agcggctcca gcacccggcg gataaaatcc
     3661 gcaggcgggg ccgacgggtt atcggtgcct ccgctcactt caacacgcga aggtccatcg
     3721 gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg tacagcttcc ggcactaccg
     3781 atagcaaagc ggtactcgcc gccgcgcacg gtgccaggcc ggaagaccag acgctgcgac
     3841 cccagctccg cgccttccac cgttgcccta caaatttccg cagccgcttt taccgcggtc
     3901 agatgctggc gcaacagccc cggtttcgct cgcccggcac gaatgctggt gatggtaaat
     3961 ggctggccgg ttatcatcga caggctcagc gccgagcgca ggatctgccc gccaccttcg
     4021 ccctgtgcgc catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc
     4081 tgacgcaggg tatagataac ttccaccaga tcgctttgtg ccgccatcac cgcatcaata
     4141 tctttatacg ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc
     4201 gcggtggcac gaatttgatc ttccacgctg aacagttttt tcgctttagt acggctcatt
     4261 acccgcccgg caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg
     4321 ataaagcttt tcgctcccat cgaaccggga ataattccat attgaccagc acgcgcagac
     4381 accgcgcctt tacgcgtcac gtagatctct tcgccaaagt gctgttcttt ttgcacatag
     4441 ttgtggtgac agttgatctc ttccatcgcc agcgtttgct gttgtcttac cgttttctgc
     4501 gtaacgctct gcaacgctgt tactacgttt tccatcattg catcgcggtt aaggctggca
     4561 aaaagctgcg cccaggccac cgctttcagg taatcatcaa agtattccgt accttccata
     4621 aagtacgcca gatcacgcga cggaaacgtt tcaagctggt cctgcatctc tttttgcgcc
     4681 agatcgataa agtaagtccc gatggcgtta ccaattccgc gtgaaccgga gtgcagcata
     4741 atccacacct gatccgactc atcaagacag atttcaataa aatggttacc cgttcccagc
     4801 gttcccagat gtttatagtt attggtattc aggaaacgcg gatatttttg cgttaaccac
     4861 tgataaccgg cttccagctc agcccattta gcatcgacgt taacaggtgg attttcccag
     4921 gcacctttat cacgtttaca acggccagtg gtacgcccgt gcggcacggc cgtttcaatc
     4981 gcctgacgca gctctgccag gttttcaggc aggtcttccg ccgttaacgc ggtacgcagc
     5041 gcgttcattc cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc
     5101 gggatcacgc taccaatggt ggaaccttta cccaggtgta catcaggcat taccgcaata
     5161 tgtttgaaaa taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcctct
     5221 accggcacgc ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa
     5281 ttcattttgt ttttctcttt tcgttgtttg ctgtcctgat aattgcaacc gtcgtgccag
     5341 aaaattaaat aagcagctta attttttaat tcattgtttt aaaaaagatt atatctttac
     5401 gtccgtaacc ggagatttcc cgcaaagcca atttaccgat aatgaaatat cgtcttttat
     5461 aaggatatct aagatgcgta aaacagtggc ttttggcttt gtcggtaccg tactggatta
     5521 tgccgggcgc ggcagtcagc gctggtcaaa atggcgtccg acactctgtt tatgccagca
     5581 agaatcgttg gtcatcgatc gactggaatt gttgcacgac gcccgctcgc gctcgctatt
     5641 tgaaacgctt aaacgcgata tcgccagcgt ttcgccagaa acagaagtgg tgagcgttga
     5701 gattgaactg cataacccgt gggatttcga agaggtctac gcctgcctgc atgatttcgc
     5761 ccgtggttac gagtttcagc cagaaaaaga agactattta attcacatca ccaccggtac
     5821 ccacgtcgcg cagatttgct ggtttctgct ggcagaagcg cgttacctgc ccgcccggct
     5881 gatacaatct tcacctccgc gcaaaaaaga acagccgcgc ggcgcaggtg aagtgacgat
     5941 tatcgatctc gatttaagcc gttataacgc catcgccagc cgctttgccg aggaacgcca
     6001 gcaaacgctt gattttctta agtccggcat tgccacgcgt accccccact tcaaccgcat
     6061 gattgagcag atcgaaaaag tggcgatcaa atcccgcgcg ccgattctgc ttaacggtcc
     6121 aaccggcgcg ggcaagtcat ttctggcgcg acgcatcttc gagttaaaac aggcgcggca
     6181 tcagtttagc ggcgcgtttg tggaagtgaa ctgcgccacc ctgcgcggcg ataccgccat
     6241 gtcgacgctg tttggtcatg taaaaggcgc gtttaccggg gcgcgggaat ctcgtgaagg
     6301 tttattacgc agcgccaacg gcggaatgtt gtttcttgat gagattggcg aactgggcgc
     6361 agacgaacaa gcaatgctgc taaaagccat tgaagagaaa accttttacc cgtttggcag
     6421 cgatcgccag gtgagcagtg attttcagct tatcgccgga acggtgcgcg atttgcgcca
     6481 gctggttgcc gaaggcaaat ttcgcgaaga tctgtacgcg cggatcaatc tctggacctt
     6541 caccctgccg ggtctacgcc agcgccagga agatattgaa ccgaaccttg attatgaagt
     6601 ggagcgccac gccacactca ctggcgacag cgtgcgtttt aacaccgaag cgcggcgcgc
     6661 ctggttggct tttgcgacct ctccccaggc aacatggcgc ggtaactttc gcgaactttc
     6721 tgccagcgtc acgcggatgg ccacctttgc cactagcgga cgcatcactc tggacgtggt
     6781 tgaagatgag ataaaccgtc tgcgctataa ctggcaggag agccgcccct ccgcgcttac
     6841 ggcgttgctg ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc aactggaaca
     6901 cgtgatagct ctctgtcgcc aggcaaagtc gctttccgct gccggacgcc tgctttttga
     6961 tgtttcgcgc cagggcaaag ccagcgtcaa tgacgcggat cggctacgca aatacctggc
     7021 gcgttttggt ctgacgtggg aagccgtgca ggatcagcac agctccagtt gaatatgatg
     7081 gtccgtcagc acctgcatta cgcttactgg cggcggggcg tcggtgtaga cggcatctac
     7141 catgctgatg ctgcccatat tgaccatcgc gttacggcca aatttcgagt gatcgacaac
     7201 cagcataacg tggcgcgagt tctcaataat ggcgcgcttg gtgcgaactt cgtggtaatc
     7261 gaactccagc agcgagccgt cgctatcgat gccgcttatc cccagaatgc cgaaatcaag
     7321 gcggaactgg gagataaaat cgagcgtcgc ttcgccaatg atcccgccat cgcggctgcg
     7381 taattcgcca ccggcgagaa taatgcggaa atcttctttt accatcaacg tgttagcaac
     7441 attgagattg ttggtgacaa tacgcaaatt gctgtgatta agcagcgcgt gcgccaccgc
     7501 ttccggcgtg gtgccgatat cgataaacag tgtcgagccg ttggggattt gctccgccac
     7561 tttgcgggca atcctctctt tttcttcggt ctgggtggct ttgcgatcgt gccacggcgt
     7621 gttaaccgaa ctggaaggca gcgccgcacc gccatggtga cgcaggatca ggttttgctc
     7681 cgccagctca ttgaggtcgc ggcgaatagt ctgcgggctg acggagaaat gctctaccag
     7741 ctcttcagta ctgacataac cctgctgttt aaccagttcg ataataccgt tgtgacgttg
     7801 tgtttgtttc atttataaat ccctggaatt attttcgttt tcgcgcattg agcgaatcaa
     7861 caaaagccat cgctaaaccc acggctaacc cggcgatgtg tgctccgttc gccatcgaca
     7921 tcccaaacaa atcaaaccat ccggcgacaa tccagatcag cgcaaagata attaacccac
     7981 gttgcaggta aatgccactt tgcggatcgc gttcaccacg tagccagacg tagcccatca
     8041 gcgcatacac cacgccagaa agcccgccaa accacggccc gctgaatttt tgctgcacat
     8101 agccgcttaa cagggcgcta atgagagtaa taacaattag cttaccgcta ccgaggcgtt
     8161 tttccaccgc accgccgaga taccaccacc agagcaggtt aaagaggata tgcatcagcg
     8221 agaagtgcat taacgcgtgg gtgaagtaac gccagaactc aaatttcagt gttggatcga
     8281 atggccaggc cagccataac atcacttcct gatcgccgag aatttgcatg gcgataaaca
     8341 ccaccacgca ggcgatcatc accacccagg ttaccggacc tgcgcgttca cgcaaggcag
     8401 caaagaaagg ataacggcga taatgcagac cactgccggt atggcccgcc tgccagctgg
     8461 ccgccagata acgcggatct gccgggtttt cgagaaaacg cgccagctcc gcccgcacgc
     8521 gctcggcctg ggactcatcc gccagccaga catcgctttg gttatgttgt tgaatcgtaa
     8581 ggataacacc ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg gggttagcaa
     8641 aagaggtaat catcaacatc gttgctgtcg cttattccac acaaaagggg acagtataaa
     8701 gcgttacgcg ccgtacgcca cctctgcggg aaactgacgc tgccaggctt caaaaccgcc
     8761 gtcaatgcta tagaccacat cgtagccctg ttgcagcaga tactgcgccg cgcctttgct
     8821 gctattgccg tgataacaca tcaccatcac cggagtgtca aagtcgttat cacgcataaa
     8881 cgcgcccagc gtgtcgttgg ttaaatggaa agcctgcacc gcatgcccca ttgcgaaact
     8941 ctgtggatcg cgaatatcga ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgac
     9001 ggcaacgtta atacattcga actgatccat gcgtctctct ttctttacaa acaagtgggc
     9061 aaatttaccg aacagtttac gtcgaagcgg cagataaacg ccataatgtt atacatatca
     9121 ctctaaaatg ttttttcaat gttacctaaa gcgcgattct ttgctaatat gttcgataac
     9181 gaacatttat gagctttaac gaaagtgaat gagggcagca tggaaaccaa agatctgatt
     9241 gtgatagggg gcggcatcaa tggtgctggt atcgcggcag acgccgctgg acgcggttta
     9301 tccgtgctga tgctggaggc gcaggatctc gcttgcgcga cctcttccgc cagttcaaaa
     9361 ctcattcacg gtggcctgcg ctaccttgag cactatgaat tccgcctggt cagcgaggcg
     9421 ctggctgaac gtgaagtgct gctgaaaatg gctccgcata tcgccttccc gatgcgtttt
     9481 cgcctgccac atcgtccgca tctgcgcccg gcgtggatga ttcgcattgg tctgtttatg
     9541 tacgatcatc tgggtaaacg caccagcctg ccgggatcaa ctggtttgcg ttttggcgca
     9601 aattcagtgt taaaaccgga aattaagcgc ggattcgaat attctgactg ttgggtagac
     9661 gacgcccgtc tggtactcgc caacgcccag atggtggtgc gtaaaggcgg cgaagtgctt
     9721 actcggactc gcgccacctc tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa
     9781 gatatcgata ccggcaaaaa atatagctgg caagcgcgcg gcctggttaa cgccaccggc
     9841 ccgtgggtga aacagttctt cgacgagggg atgcatctgc cttcgcctta tggcattcgc
     9901 ctgatcaaag gcagccatat tgtggtgccg cgtgtgcata cccagaagca ggcctacatt
     9961 ctgcaaaacg aagataaacg tattgtgttc gtgatcccgt ggatggacga gttttccatc
    10021 atcggcacca ccgatgtgga atacaaaggc gatccgaaag cggtgaagat tgaagagagt
    10081 gaaatcaatt acctgctgaa agtgtataac acgcacttta aaaagcagtt aagccgtgac
    10141 gatatcgtct ggacctactc cggtgtgcgt ccgctgtgtg atgatgagtc cgactcgccg
    10201 caggctatta cccgtgatta cacccttgat atccatgatg aaaacggcaa agcaccgctg
    10261 ctgtcggtat tcggcggtaa gttgaccacc taccgcaaac tggcggaaca tgcgctggaa
    10321 aaactgacgc cgtattatca gggcattggc ccggcgtgga cgaaagagag cgtgctgccg
    10381 ggtggtgcca ttgaaggcga ccgcgacgat tatgccgctc gcctgcgccg ccgctatccg
    10441 ttcctgactg aatcgctggc gcgtcattac gctcgcactt acggcagcaa cagcgagctg
    10501 ctgctcggca atgcgggaac ggtaagcgat ctcggggaag atttcggtca tgagttctac
    10561 gaagcggagc tgaaatacct ggtggatcac gaatgggtcc gccgcgccga cgacgccctg
    10621 tggcgtcgca caaaacaagg catgtggcta aatgcggatc aacaatctcg tgtgagtcag
    10681 tggctggtgg agtatacgca gcagaagttg tcgctggcgt cgtaaggatg atgcccggtt
    10741 gtgaagtgca accgggctat ttggattcct tagactattc attgtaaata attgaataac
    10801 ggcccctgag ctaaaatcgc caaacttaag cacgttaccc aatcgcccca cttcttcctc
    10861 tggtttctat tttttctgca ccaatggcaa taatatccca atgcaaaacc aagctgtgca
    10921 ctaatcgggc cgacaaaatc agtccaaggc tgcggccaaa aaggggaaaa attcagggga
    10981 agccagacaa acgtcagaaa gaaatttcgg atattttcca gcaacgctga tttctctgga
    11041 gaaatataaa gaatgaaatt cgagagaatt aaccagaaat agaccggcga aaagaccagc
    11101 cagcaatgca gtctgttttt taattcagga ggcgttctgc gggagaattg ccaaccgatg
    11161 ccagccatta cactgaaaaa aagtgcatat tcaaccatta actcaagttg tagttcctga
    11221 agagaaatac gcacaaccag gagcggaaag gagaatatta tttctgccag aaaagttaac
    11281 gtgaatataa gtacggctaa gacaataacc gtcggccata ccaataatgg cacgataaca
    11341 aaaccgagaa ttacaggggg aatataaagc cggaatttaa tcatttttat tttcctggtg
    11401 attcggatga tgcgtcatac atcgcgcata aacactcatc aattatccag tcgtctaccc
    11461 agacctgatg ctgtgtgccc tgttctggct gctttggtgg gttctcaatc gcctgccgcg
    11521 cgcgttcttt caagatggat tgattgttga aggcccaggc attgatttgg gtggtaatga
    11581 cgttatcgat atcgtcctgg ttatctctga tataatcctc acaatatctg ttcaataaac
    11641 aggcaagaga aacggtcgac caaaagccgg gaactgaatt aatggcatcc agcaatggcg
    11701 caagagcgat atcggcacgg aaaaacattt ccagcatata ctgtaattcg acagcttcat
    11761 cgccggatac ccaatcagtc atatactggc aggcaaatcg ttgcaaaatt ttccattcat
    11821 cctcacgcca attttcttta ctggcaaggt ttatacgccg tagagaaaat acttcgtgaa
    11881 tattgtcaaa ctgcccctgt gcaacaaatt ccagtaaacg cggcagaaaa tagcggacct
    11941 catcgctatt ttgcggatca ggccctgggc tactattcca tgcattgatt aaggaatacg
    12001 agatcgctcg taacgatgta ctccgtaata ccttttgttc ctgctgactc aggcaatatt
    12061 cacaacaaac cacaaatttc tgcggcaccg gataacgacg aaaaatatga taaatctgct
    12121 caattaatgc ctgcatggcg gggctgggtt gcataattac cccctcgccc gctgatcgcg
    12181 tgtaaacggt tgataaacaa aacgagcatt cgccacgttc aggcgaataa tggcactacg
    12241 agagagggaa aagctggggc gaatgcaact attgcagtct cgacagcttt cttcagcgca
    12301 attttccact gtgtaataac gagcgtagag tttcagttcc gcttgcttat cacgaaatcc
    12361 gcgtaaactg ttatcgttat gaaatttcca ggcatcttta aagacctgaa aaatgtagat
    12421 atcctcaatc caggactcac attttttaag cgggcgcttt gctatgatca cccgctcatc
    12481 cgggtttagc atcgccgtaa attgaacggt gaggtcattt gtcgtgttca gatcacattg
    12541 aacatctggt acatcactga tataggggtc aaatccaaag tcagcaccac aggcacaaag
    12601 acggctaatg gtggcggtat cgagggaaaa tcccggccat acattgctgg acgttaagcc
    12661 aatatttaat gtgacattat atcgaagcaa tccctcaaga ccgtcagcac cgacgaactt
    12721 ttccccttca tccagggcct ggttaatgag ttgctgaaca atgccttcat cccagtatgc
    12781 cgatttgcag ttggcaatcg taatatccac gcctgtgaat ggatcagtac cgacaaggct
    12841 tgcaaataat gtattaacta attccatttt tattatccgc cttaatgcaa cgttttatac
    12901 atcggcacat aacctacatc ttccaccagg ctctgcccct gcggcgttaa aaaccactcc
    12961 accaattttt gtgtttctga cgtcatattt tcccgcgtta ccataaaggc atcgacgata
    13021 tagggatatt tgccgttgcg aatgttttcg gcagtcggtg caataccgtt aatcgctagc
    13081 agttttatat ttttatcggc gttcatttgt gttgcgtaat aacggaaggt gtaacctatt
    13141 gcgttgtttg tattacggta ttcggcaacg actttaatca ttccctccat catacttgcc
    13201 acttctgttt cctgcggcga gatcatgcgg acatttttca tgacctggga ttgcatcacc
    13261 gtctggctac cagagtcttc cgggcgttgc caggtctgga tctcctgatc gttaccgcca
    13321 accgtgcgcc agttggtaat tgcaccactg aagatgtcac gcacttgttg ttcggtcagg
    13381 gaattaaccg ggttatctac attgacgatg aaaacaaacg cttcacgggc aaaaggcgtg
    13441 taaatcaaag tgacgcccga ttcctccgcg cgttttttct gcccaccgga aggttgagcc
    13501 acaaagataa tatcggcatt acctttaaca atcttgttat atgcttcggg agtacgagag
    13561 ttcgccagat attcccattc gtgaaaatct tccggcaaaa cgcttagtgc ataaaaggca
    13621 gaggcataaa tggggtacgc cgccgttgcg ccatcaagtc gcggccagtt ttgcgtgaac
    13681 tgaatttgcg gtttaccacg cagtggtgtc agttgattat tgagtttatc gggtcgccag
    13741 gcccaggtat caatattttc acgcacaccg acgccgggat taagctgttt atataaataa
    13801 gcctggtaac cggcgacaaa accaagcatg agaaggataa acaagagaga attacggcaa
    13861 cgtaacccgg acgtgcccgt taaaggatat ctggtcggcc agccgtaccc cagcgaaaaa
    13921 ctgatatgcg cgccaacagg tataatgact aaccaccagt actcaccacc gactaatgag
    13981 gctaacaaaa cgcccagcca ggggagcata agatcagcca accaacccgc tcccatctgg
    14041 cgaaattgat aatctgccag atgcagcgcg attatccaga cccccagggt taataatgcg
    14101 ggtactaaaa aaagacattg tcgccagacg gctatttctc ttttttctgg tgcactatcg
    14161 tccgcccaca ctcgccccga gaaaaatagc attaatgcgc ccagcgccag cataaaaatg
    14221 ttgagcagaa ggtaaattgt tggcgtgata aagatattac aaacttcaat aataccggca
    14281 agtccgacac ccagcatggc aataaccgcc gccaaaaact gcgccagaat ggggatgccg
    14341 aaaaaagtca ttaccagcga ggtcaaaatc cattttctgt tttgcattat tctttccatt
    14401 ctttctgaat ggcgaaatta tactccccga gtccccttgc cccttctgga cacttttccg
    14461 aaatgatggc gaaaaaaacg ggaccctttg gccccgttct atttattagt gaaattacaa
    14521 tctcaccgga tcaatatgcc agatatgatc ggcgtactct ttgatagtac ggtcagaaga
    14581 gaagtagccc atattggcaa tgttcagcat cgcttttgcg gtccactctt cctgaagctc
    14641 gtagagttcg tcgactttat cctgacaatc gacatagctg cgataatccg ccagaacctg
    14701 atagtgatcg ccgaagttga tcagcgaatc aaccagatcg cgatagcgac ccggatcttc
    14761 cggactgaat acaccgctgc cgatttgcgt cagcacctga tgcagctcct catctttctc
    14821 gtagtattca cgcggtttgt agccctgacg acgcagttct tccacttctt ccgcagtgtt
    14881 accaaagata aagatattgt cagcaccgac atgatccagc atctcgacat tcgcaccgtc
    14941 caacgtaccg atagtaagcg caccgttaag cgcaaacttc atgttactgg tgccggaagc
    15001 ttccgtcccc gccagcgaaa tctgttcaga cagatcggcc gccggaatga tcaactgcgc
    15061 caggctaacg ctgtagttcg ggatgaacac gactttcagc ttgtcgccaa tctgcggatc
    15121 gttgttgatc actttcgcta cgtcattgat caaatgaata atgtgcttcg ccatgtaata
    15181 ggccgaagcc gccttaccgc caaaaatatt cacgcgcggt acccacttcg catccgggtc
    15241 ggccttgatg cggttatagc gggtaatcac atgcaacaca ttcatcaatt gacgtttgta
    15301 ttcgtgaata cgtttgattt gtacatcgaa caacgccttt ggattcacca ccacattcag
    15361 ttgctgggcg atatactctg ccagacgctt tttgttctcc agcttcgcct gatgcacagc
    15421 gtgattaacc attgggaaat cacagtgttg ttgcagctca ttaagcaggc taaggtcggt
    15481 gcgccagtta cggcccaggt gttcgtccag cacggctgaa agcgatgggt tcgctaccgc
    15541 tagccagcga cgcggcgtca caccgttggt gacgttggtg aaacgacccg ggaagatttt
    15601 cgcaaagtcg gcaaacaacg attgcaccat cagattagag tgcagttccg atacaccgtt
    15661 aactttgtgg ctcacaacaa ccgccagcca ggccatacgc acacgacgac cgttggattc
    15721 atcaatgatc gacgcccgtc ccagcagatc ggtatcgttc ggatactgtt cctgcaaggt
    15781 tttcaggaaa tagtcgttga tttcaaagat gatctgcagg tgacgcggca gaattttacc
    15841 cagcatatca accggccagg tttccagcgc ctcgctcatc agcgtgtggt tagtgtagga
    15901 gaagacctga caacacacct caaacgcgtc gtcccagcta aattggtgct catcgatcag
    15961 cagacgcatc atctcaggaa tcgacagtac cggatgggta tcattgagat gaatcgcgat
    16021 tttatccgcc aggttatcgt aggttttatg caactgataa tggcggctta aaatgtcctg
    16081 aatggtcgag gaaaccagga agtattcctg acgcaggcgc agctcacgcc cggagtaggt
    16141 ggagtcatcc ggatacagta cgcgagatac gttctcggag tggtttttat cttccactgc
    16201 cgcgaagtag tcaccctggt tgaatttacc gaggttaatt tcgctactgg cttgcgcact
    16261 ccacaaacgc aacgtgttgg tcgcgtcggt gtcgtaacca gggattatct gatcgtaagc
    16321 gactcccaga atctcttcgg tttcaatcca acgcgttttt ttaccttcct gctgaatgcg
    16381 accaccaaaa cggactttat agcgcgtgtt gtggcgtttg aattcccacg ggttaccgta
    16441 ttccagccag tagtctggcg actctttctg gctaccgtta acgatgttct gcttgaacat
    16501 accgtagtca tagcggatgc cgtaaccgcg ccccggcaac cctaacgtcg ccagagaatc
    16561 aaggaagcaa gccgccagac gtcccaggcc accgttaccg aggcctgggt cattttcttc
    16621 atcaatcagc tcttcgagat ttaaccccat cgcttccagt gcgccctgta catcttcgta
    16681 aattcctagc gacaacatgg cgttggagag cgtacggcca atcaaaaact ccatcgacag
    16741 gtagtaaacc tgacgagttt cttgcgacaa ctgggcacgg tttgaacgta accagcgctc
    16801 cacgagacga tcgcgcacag caaataacgt tgcgttcagc cattcatgtt tattggcgac
    16861 gaccgggtcc tttccaatcg taaacatcag cttgtaagcg atagagtgct taagagcttc
    16921 tacgctaagc gtgggcgatg aatatgtaaa cggagcattc atataggcgt ttcctgaaaa
    16981 ctatttcaag cgatagtaaa gctcacggta cgacttcgcc gcgacctgcc agctaaaatc
    17041 cattgccata gcctgacgtt gcacaaaccg ccacagtgaa ggacgggacc acagtacaaa
    17101 agcacgtcga atagcccgta acagcgacca ggcattacta tcttcaaaga caaacccact
    17161 ggcgacgcca tctgcaaggt tctcaagaga acagtcagaa accgtatcag caagcccacc
    17221 ggtgcgccgc actaacggca gcgtaccgta cttcaatcca taaagttgcg ttaagccgca
    17281 cggttcaaaa cggctgggca ccagaatgac atccgcgccg cccataatgc gatgcgaaaa
    17341 tgcttcgtga tagccaatct gaacgcccac ctggccgggg tattccgctg ccgccgcaag
    17401 gaaaccttcc tgcagcaccg gatcgcccgc gccgagtagc gccagctgcc cgccctgctc
    17461 cagaaggccc ggtaaggctt ccagcaccag gtcgagacct ttctggctgg tcagacggct
    17521 caccactgca aaaagcggca ctttatcatc aaccttaagc cccattgcga tttgtaactg
    17581 gcgcttattt tccgctttat cttccaacgt atcgcgggtg tagcgcgagg ccaacagtaa
    17641 gtccgtctct ggactccaga ttttctcgtc cacgccgttc agtacgccgg aaagacgccc
    17701 ttcacggtga cgctgttgca acagaccttc cataccgtag gcaaactgcg gttcggtgat
    17761 ctcgcgagcg taggttggac tgaccgccgt aatgtgatcg gcatagtaca gaccggcctt
    17821 caggaaagag atttgtccgt tgaattccag cccatgaata ttaaagaatg accatggcaa
    17881 ttggatgtca ttcatatgat gtgcataaaa catgccttga taggccaggt tgtgcacagt
    17941 aaacaccgac ttcgccggac gcccgcgcgc cgccagatac gcaggcgcaa ggcctgcatg
    18001 ccagtcgtgc gcatgcacca catcaggacg ccagaatggg tcaagcccgc tggccatttc
    18061 tgccccaacc caccccagca gcgcaaaacg caatacgttg tcggtatagg caaataagtt
    18121 ggtatcgtga tacgggcttc ccggacgatc atagagatgc ggcgcgtcaa tcaggtatat
    18181 gccaaccccg ttgtaatgac cgaacaacag cgtgatatgt ccggcgaagg tatcacgacg
    18241 ggatactacc tgcgcatcgg taacgccacg gcgaatatcg ggaaatgcag gcaacagtac
    18301 gcgagcgtca acgccgtctg cgatttgtgc tgcgggtaat gccccaataa catcagccag
    18361 accgccggtt ttaagcagcg ggaacatctc tgaacataca tgtaaaacct gcattatcgc
    18421 tcctgtttat gccctaactt ccgtagcatt tcgcgcgtta ccagcacgat gccttcttct
    18481 gaacgataga aacgacgtgc atcttcctct gcgttttcac caatcaccat gccttccgga
    18541 ataacacaag cacgatcgat gacgcagcgg cgcagacggc acgagcgacc tacccatact
    18601 tccggtaaca atacggcgga atcaatgttg cagaatgaat tcacgcgaac gcgcgagaac
    18661 agaacggact gcaccaccac cgaaccggag atcacacagc cgccggatac cagtgagtta
    18721 agggtcatcc cgtggctacc ggagcgatcc tgcacgaatt tcgctggcgg taatgattca
    18781 ttgtaggtgc gaattggcca attgcgatcg tacatatcca gttccggcac cacagaggcc
    18841 agatcgaggt tcgctttcca gtaagcttcc agcgtaccca catcgcgcca gtacggctcg
    18901 gcatccgggt cggattgtac gcaagagagc gggaacgggt gcgcataggc cagaccggct
    18961 tcggtgatct tgggaatcaa atctttgcca aagtcgtggc tggagttctc atcacgatcg
    19021 tcttcttcca gcagttcata cagatagtcg gcgtcaaaga cgtagatacc catactcgcc
    19081 agagatttgc tcggatcgtt cggcattgac ggtgggttag caggtttttc aacgaattcg
    19141 ataattttat cgttctcatc aaccgccata acgccaaatg cggaggcttc ttcaatcggt
    19201 actggcatac aagcaacggt gcaacgtgcg cctttttcga cgtgatcgat aagcatacgc
    19261 gagtagtctt gcttgtagat atggtcgccc gccaggatca ccacgtattc cgctttataa
    19321 cggcggataa tgtcgaggtt ttgggtgacc gcatctgcgg tgccgcgata ccagttttcc
    19381 cctttcattc tctgctgtgc tggcagcaga tcgacaaact cgttcatttc ttcattgaag
    19441 aatgaccagc cgcgctgaat gtgctgcacc agagtgtggg actggtactg ggtgatcacg
    19501 cccatacgac ggatcccgga gttgatgcag ttagacagcg caaagtcgat aatgcggaac
    19561 ttaccgccga agtgtacggc cggttttgct cgcttattgg ttaaatcctt caggcgggta
    19621 ccacgtcctc ccgccagtat cagggcaaca gatttcaatg gcagctggcg cgccaacatt
    19681 aagtgatcgt tcttctctaa actaaccatg actaactcct tttttatcat ctctggaaca
    19741 cacacaatcc gtgtgcaggt ccctgccaga cagccgtaat cactgggtta tcctctccag
    19801 cgaatggggg aatggcgtgc cactccccag caggtaaaac aatctctgtt acctcaagcg
    19861 tggcgttaat tgcgatcaaa aagcgatccg agagcagaat ttgcagctgt ttcggcccgt
    19921 tttgccactc atccgtgctt aaaggttgag catatcgatt tagccaacgg acattgccgt
    19981 cgccttcttc ccaccagcga ttctccacca aagcgggaat gcgcttgcgc agatggatta
    20041 acgcggcggt aaatgcggtt aaaccactgc ttgcctgcga ccagtccaac caggttaatt
    20101 ggttatcctg acagtaggca ttgttattgc catgctggct gtgaccatgt tcgtcaccgg
    20161 ccagtaacat cggcgtaccc tgggagagca acaacgttgt taacagggcg tgaatgctgt
    20221 cgcgccgccg ttcaaccagg tcaagagaac cgcctaaccc ttctttacca tgattgttac
    20281 tgtaattgtt gttggtcccg tcgcgatttt cttctccgtt tgcttcattg tgtttatggt
    20341 tgaagcaaac gcagtcgcga agcgtaaaac cgtcatgcgc ggtgacgaga ttaatcgcgg
    20401 cactcggcag acgaccatta cgtttaaaaa catcgctgga ggcagcaaaa cgcccggcaa
    20461 acgcccccag aggcaaatca taatgtagcc agaaacgacg ggcagcatcg cggaaatgat
    20521 cgttccactc ggcaaacagc ggcgggaaat ttcccacctg ataaccacca ggagcgatat
    20581 cccacggttc agcaattaac ttcacctgcg agagcaccgg gcagttctgg atagcggtaa
    20641 acaacggcgc atcctgacgg aactctggcg tacggcccat gactgccgcc agatcaaagc
    20701 ggaaaccatc gacgtggcag gtttctaccc aataacgcag gcaggcgctg gcataatcca
    20761 ccaccgccgg atgactcaaa ttgagcgtgt tgccgcaacc ggtccagttg tgataatcgc
    20821 cgtcttctct tatccaataa tagctacggt tatcgatccc acgcagcgag aataacgggc
    20881 cgtcgaggtc cagttccgca ctatggttga gcacgatatc aagaatgact tcgatacccg
    20941 ctttatgcag tgctttgatt gcatcgcgaa actcatccag cgccgtttct ggcgagcagg
    21001 catacgccgg atgcagcgca aacatcgcca ccgggttgta accccagtag ttacttagcc
    21061 ccatgcgttg cagacgtggt tcactggcaa actgcgccac tggcagcagt tccagcgcgg
    21121 taatgcccaa ttgtttcaaa tagttgatca tcaccggatg cccgagggct ttataagtgc
    21181 cacggatctc gaccgggatc tccgggtgca agtacgttaa tcctttgaca tgggcttcat
    21241 aaatgatggt gctgccccac ggcgtgcgcg gcggggcatc atcttcccag tcatagtgat
    21301 caaccaccac tacgcatttc ggcgcaatgg cggcgttgtc gcgatagtca ggttcattat
    21361 gaccggcgtg cagcagcggg ttatctttaa actccccctc aatttgccgc gcgcaaggat
    21421 caatcaacaa cttcgccggg ttaaagcgat gcccctcggc gggttgccag gggccatgaa
    21481 cgcgataacc ataacgcaaa cccgggcgcg catccggcag ataaccgtgc caaatgtcgc
    21541 cactgtgccc tggcaagtca tagcgatgtt cctggccatt ggcgtcaaag acacacagtt
    21601 ctacccgctc ggcatgagcg gagaaaagtg tgaagttgac gccctgaccg tcgtaatgcg
    21661 cgccgagggg agcgggtttg ccaatggcga gttgtgtcat tctgcctccc gaaccagcca
    21721 gatagtggcc agcggtggta gcgtcaggct tagtgaatgc tgacgaccgt ggctggcaat
    21781 ctcatcgctg tgtaccgtgc cgccattgcc tgcattactg ccgtgatagt gcatggaatc
    21841 ggtattgagg atttcacgcc atttgcccgg ctggtttatg ccgaagcgat aatcatgacg
    21901 cggcaccggc gtaaagttac tggcaacgat gatttcgtta ccctctttat cgcgacgcac
    21961 aaagatcagc accgagcgtt ctttgtcatc caccaccagc cattcaaagc cgtacgggtc
    22021 aaaatccagt tcatgcattg ctttatggtg gcggtaggtg aggttcagat cgcgcaccag
    22081 acgctggaca ccgtggtgcc agttatcgcc gccttccaac agatgccagt cgaggctggc
    22141 gtcatggttc cactcgcggc cctgggcaaa ttcgttaccc atgaacagta gtttcttgcc
    22201 cgggaatgcc cacatccagc catagtaggc gcgcaggttc gcgaatttct gccatgcgtc
    22261 gcccggcatg cggtcgagaa tcgatttttt accgtggacc acttcatcat gcgacaacgg
    22321 caggacgaag ttttcagtgt agttgtagag aatcccgaag gtcagtttat cgtgatgata
    22381 ctgacgataa accgggtcga gcttcatgta gtccagggtg tcatgcatcc agccgaggtt
    22441 ccacttgtac cagaagccca gaccgcccat atcctgcgga cgagaaacgc cagggaaatc
    22501 ggtagactcc tcagccattg tcaccgcacc ggaaacctgc tcaccaagaa tacggttggt
    22561 attacgcaag aattcaatcg cttcaagatt ctcgcgcccg ccaaattcgt tcgggatcca
    22621 ctccccctct ttacggctgt agtcgcgata aatcattgac gccaccgcat cgacgcgcag
    22681 cgcatcaata ccaaaacgtt caatccagta aagcgcgtta ccgacgagga agttactgac
    22741 ttcacggcga ccatagttgt agatcagcgt gttccagtcc tgatgatagc cttcacgcgg
    22801 atcgctgtgt tcatacaagt tcgtgccatc aaattcggca agcgcaaagt catcagtcgg
    22861 gaagtggcct ggcacccagt cgagaatcac gttcagacca gctgcgtgtg cggcatcaat
    22921 gaaataacgg aagtcgtcgc gagtaccaaa acggcgggtt ggtgcataca ggccggttgg
    22981 ctgataaccc caactgccat cgaagggatg ctcgttaatg ggcagtagtt cgaggtgggt
    23041 aaagcccatc catttagcat aaggcaccag ttgatcggcc agctcgcggt agctcaacca
    23101 gaaattgttg tcggtgtggc gacgccagga acccaggtga acttcataaa tagagattgg
    23161 cgcatcaaac tgattcgctt ttttgcgctc ttcagtctgt acaacctttt ccggcagccc
    23221 gcaaataaga gacgcggttt ccgggcgcat ttgcgcttcg aaggcataag ggtcggactt
    23281 cagacgcaag ttgccattgg catcaatcat ctcgtattta tagagctgac cgttatgcgc
    23341 cccagggata aacagttccc agatgccgct ctctttacgc aggcgcatcg ggtgacggcg
    23401 accgtcccag tagttgaatt gcccaaccac cgagacccga cgggcgtttg gagcccagac
    23461 agagaaacgc gtacctgtga cgccatccat agtatctgca tgcgcgccta aggtttcata
    23521 cgggcgcagg tgagtacctt cagataatag ccaggcatcc atttcctgga ttagcggacc
    23581 aaaacggtaa ggatcatcaa tcaggttttg ctgaccatgc cagacaacag ccaactgata
    23641 gcggaaaaaa ttcttacgtc gcggaatgac gccgctaaag aatccccgtg agtcgagaca
    23701 ctccagtttt gcgagtttgc gcccggtttt cggttcaatc acccacacat cggtagcgtc
    23761 gggtaaaagg gcacggactt ccagtcccgc ggtggtttta tgcattccca gtacggaaaa
    23821 aggatccgca aaatggcctg caattagcgc gttaatcacg tctctatcga tacgatcgga
    23881 catgcttgtc ttcctgtttt attgtgtcac cccatccaat tggatttttg acttctggtt
    23941 gtgacatttt tttgacctga acggcgcagc actctgtgca tcctctctgc gtcgtcctca
    24001 cttcaggtaa ggctgtgaat actcatgtat tcagccaccc ttaaagaata gccaatgctc
    24061 tatttaactc ccggtaaatc atgaaacatc tgcgcttact cctgtattac gcactaacag
    24121 gggcggcatc gcgccccaga tttaatgaat aaagattacg ccagttgacg aagcatccga
    24181 cgcagcggct ccgcggcccc ccacagcagc tggtcgccca cggtaaaggc tgacaggaac
    24241 tctggtccca tattcagctt acgcaggcgg cctaccggcg tggtcagcgt gccggtaacg
    24301 gcagctgggg ttagctcacg catagtgatt tcccgatcgt tcggaacgac tttcgcccac
    24361 ggattgtgcg cagccagcag ttcttccacg gtcggaatag acacatcttt tttcaattta
    24421 atagtgaatg cctggctgtg gcagcgcaat gccccgacac gcacacataa accatctacc
    24481 ggaattacgg aagatgtgtt gaggatcttg ttggtttccg cctgcccttt ccactcttcg
    24541 cggctctgac cgttatcgag ctgtttgtcg atccacggaa tcaggctacc cgccagcggc
    24601 acgccaaagt tatccaccgg cagctcaccg ctacgggtta aggttgtgac tttgcgttcg
    24661 atatcgagaa tagcagagga cggggtcgcg agttcatctg ccacatggcc atacagatgg
    24721 cccatctggg ttaataactc acgcatatgt cgcgcaccac cgccggaagc ggcctggtag
    24781 gttgcaacgg acacccaatc aacaagatca ttggcgaata aaccacccaa cgacatcaac
    24841 atcaggctta cggtacagtt accgccaaca aaagtcctga tgccattatt taatccgtcg
    24901 gtaatgacgt cctgattgac ggggtcaaga atgatgatgg cgtcatcttt catgcgcaga
    24961 gacgatgctg cgtcaatcca gtaaccttgc catccgcttt cacgaagctt tggatagatt
    25021 tcgttggtat aatcgccgcc ctgacaggtc acaatgatat cgagggcctt tagcgcctcc
    25081 agatcaaagg catcctgaag tgtgccagtg gttccgccaa aagacggcgc agcctggcca
    25141 agctgagaag tagaaaagaa gacagggcga atggcgtcga agtcgcgctc ttcaaccatg
    25201 cgttgcatga gaacggagcc gaccataccg cgccagccga taaaaccaac atttttcata
    25261 agcgtttttt tcctgcaaag atgtgtgctg tataaatgtg ccggtctcct cttggcacat
    25321 ctttcaccat acaaaaagca gccaaagtcg caagtgaaat taatcaatga tagcgaagcc
    25381 atcagtaatg cgacttatcc tgctttgtta gcacgcagaa agtccgcggc aattatcagg
    25441 gaatttgagt tatgaatgaa atcatttctg cagcagtttt attgatcctg attatggatc
    25501 cgctcggaaa cctacctatt ttcatgtccg tactgaaaca tactgaaccg aaaagacggc
    25561 gggcaatcat ggtgcgagag ttgcttattg ctctcctggt gatgctggtg ttcctgtttg
    25621 cgggtgagaa aattctggca tttctcagcc tgcgagcaga aaccgtctcc atttctggcg
    25681 gcatcattct gtttctaatt gccattaaga tgattttccc cagcgcttca ggaaatagca
    25741 gcgggcttcc ggcaggtgaa gagccattta tcgtgccgtt ggcaattccg ttagtcgccg
    25801 ggccgactat tctcgccacg ctgatgttgt tgtctcatca gtacccgaat cagatggggc
    25861 atctggtgat tgctctgctg ctggcctggg gcggcacctt tgtcatcctg ctacagtctt
    25921 cgctattttt acgtctgctg ggcgagaaag gggtgaacgc acttgaacgc ctgatgggat
    25981 tgattctggt gatgatggca acccagatgt tcctcgacgg cattcgaatg tggatgaagg
    26041 ggtaacgagg agagtttttc gaaaagaacc cggtagcgtc acaactaccg ggcgaacaaa
    26101 cttaactcaa cagctggaac gcaatcatcc caacgatggc accgacagtg ccgaggatgg
    26161 tttccatcat ggtccaggtt ttcagcgttt cggcttcggt cgcgccggta aatttaccga
    26221 acaaccagaa accggcgtcg ttaacgtggc tgacaacaat cgaaccacca gcgatacaaa
    26281 tcgacagcgc cgccatttgc gcaccggagt agttcagttg ttcaataacc ggcatcacca
    26341 gtcctaccgc cgttaaacag gctacggtgg cagaaccctg aatgatgcgc actgcagctg
    26401 ccagcacgaa gcaggtgata gcaatcggca ggcccatgcc ggttaacgct tcgcccagtg
    26461 ccggacctac gccagagtca accagcacct gtttgaacac gccgcctgca ccaatcacca
    26521 gcagaatgat ccccgccggt tgcagcgcgt gaccgcaaat ctccatcact ttgtcttttg
    26581 gcatgccctg acgcattgcc aggccataaa tcgctaccag acaagcaacc agaatcgcgg
    26641 taaacggatg accaataaac tcgaaccatt cgtaagcggt agagccttct ggcacaaaac
    26701 gcgcggcaat ggttttcagc cccaccagca ccagcggcaa caggatcagc gacaggctga
    26761 atccgaaaga tggcattttg ccttcgccga gatgcggttc gctgatgtcg tcaggaatat
    26821 gcaactccac gtagcggctg atgaaattac cccacagcgg cccggcaata atcattcccg
    26881 gaattgccgc acacaggcca atcaggatca tccagccaaa atcggcgttc atctgcgatg
    26941 ccagcagcat tggcgctggc cctggcacca ggaacgccgc cgctgccgcc acgcctgcga
    27001 ataatgggat taccagcttc accaggttcg taccggtgtg gcgcgccatt gagaaagcaa
    27061 cgctaatcag cagaactatc gccacttcaa agaacagcgg tagcgcacag accaaccccg
    27121 caaggccgat ggcaaaatgc gcgcggctgt gaccgaagga tttgagcatt ttgacggcaa
    27181 tctgatcgac tgcgccggtt tcatgtaaga tcttgccaaa catagctccc agggcgacaa
    27241 ccaccgccag gaagccgagg gtgcctccca tccctttttc catcgtcgct gcgattttat
    27301 cgagcggcat accggaaaaa aggccagccc ccatggacac caccatcaaa gccaggaaag
    27361 cgtgcatacg cgccttcatg actaaaaaca gcagcagtaa aacagaccct actgctgtta
    27421 aaacaagcgt taatgtagtc actacttatt tgcctttttt aataacctca atggtgcttg
    27481 ccacaacacc ttccagcggt tgatcgatat ccaccaccag tacatcggtt tcgtccgcac
    27541 ccggctcctg cagcgtttca aactgcgtca ccaacatttg ggttttaaag aaatggcctt
    27601 tgcgcgcttt caagcgactt tcaatcacat caaaatcgcc tttcaggtag atgaaagaga
    27661 gattcggatt accttcacgc agcaagtcgc gatagtgctt tttcagtgca gaacagacga
    27721 tcagcgacac tttattggtg cgctgcatag caaacgcggc gtcgttcagc gcctgcagcc
    27781 acggtttgcg atcgtcatca ttcagcggtt cgccagacgc cattttttcg atattgcgcc
    27841 gtggatggag gaaatcgcca tcaagaaacg cggcatgaag ttgatgcgcc acttcactgg
    27901 cgaccgcaga tttgccgctg cccgatacgc ccatcaagac gtaaatgtgg tgatcatggt
    27961 tagtcgtgct caaagtggtg cccccacaat acaagaatta aaaatgttac gggtaactgt
    28021 tatcggtaac attgtccagc cggacaatat cagaagcaat atccatccgt gccttaagtg
    28081 tataagtgtg agctacttca aatttgtggg cttaaataga tccgcccggt gacaaggtga
    28141 aacctaaatc taacattttc ggtgtcacag attcgccacg aatacgcgcc agcaggcgtt
    28201 cagcgccaat actgcccatc cgctcacgcg gcgtcagcac gctcgccagt cgtggctcca
    28261 tcacctgacc aatgtcatga ccgtggaaac cggcaatcgc catatcgtca ggaactttta
    28321 accccagacg ctgacattca aacgccgcgc cgaccgccag atcatcatta gtacagaaca
    28381 cgccatccag ctgcggatat tcccgccgcg cctggcgaat cagttcaata ccggaagagt
    28441 aagaagaaga ttgctcaacc atcacgctat acggcaccag gcctgcatcc agcatcgcct
    28501 gttcgtatcc cttctgtttg atgatagtac gttcgtcgag acgtgcgccg agataggcaa
    28561 tgtggcgatg cccgcgcgca ataatggcgg tagtcatctg gcgtgccgct tcaaagttat
    28621 caaaaccgac ggcgatatcg aggcacggcg actggctgtc catcaactcc accaccggga
    28681 tccccgccac ttcaatcatc tttaaggtgc gcggcgtgtg ggtacgttcg gtgaggatca
    28741 ggccgtcgat attccaggac agcatggatt cgagacgttc ttgctccatt tccggtttat
    28801 aaccgtagtg cgccagcatc gtctgataac cgtgcgcgtc ggtgacgctt tcgattccgc
    28861 gtaatacttc cgcgaaaacc tggttggtga gagaaggtaa caggacgcca atcgcccggc
    28921 tggtagcgtt agagaggata tcgggcgcac gattgggaat atagcccagt tcatcaagag
    28981 cggcggcaat cttgccgcgt agagcgacgg aaacctgctc cgggttgcgt aaaaaacggc
    29041 tgaccgtcat tttggtcacg cctacacggt cagccacatc ctgaagtacg ggtcttttct
    29101 ttttcatcgt cctgaaggta caaaagagat agattctctt agtttaacac ggacgcatca
    29161 gaaccttccc ggggaaaaca gtgcttgttt acaattatta gatatacact aaatcaattg
    29221 catcgcattg tgctaacgac gcggcaaaac cccggaagcg tgcatcagca cgcgaccgga
    29281 gcgagcgagg aagcctgcaa aaaggcaggc ttcaaagatg ccgttgagtt ttaaaccggc
    29341 ggcagatcga acagtaacac ttcgctgtcg ctatccgcat ggatggagat tgcctgctca
    29401 tcccagattg ccagaccatc gctggtcgag gctttcacgc cgttaatggt gacattgcct
    29461 ttcaccacct ggatccagac gcggcgttca gcggcaatct gatgcaccga ctgctcatct
    29521 ttcagcaacg cccaacgata cagttccata tcctgatgca ctttcagcga accatctcgc
    29581 gcatccggcg agagcaccag ctgtttgccc tgcacggcat cgaagcgacg ctgttcataa
    29641 cgcggcgtaa tgccattttc ttcaggcatg atccagatct gatacagatg cagacgctcg
    29701 gtgctgtttg ggttgtactc tgagtgacga atacccgtac ccgcactcat aatctggaac
    29761 tcacccgccg gaacctgctc tttattgccc atgctgtcct gatgctcaac agtaccttcc
    29821 agcacgtagg tcaaaatttc catatcttta tgcgggtgag tgccgaagcc ctgccctgct
    29881 tcaatcacgt cgtcgttaat cacgcgcagc gcggagaagc ccataaagtt cggatcgtaa
    29941 tagttggcaa aagagaaagt atgccaggag ttcagccagc catgatttgc atgaccacgt
    30001 tcatttgctt tgcggaagta gatcatttgt attcgccccc tgaatgattt cgatgggctt
    30061 agtgtggacc cgatctgcct gggatgatag agggtgaaaa ttgacccctc tgttcaaaaa
    30121 aattgaacta ttcgaggggc catcaaactt acttagcgag agttactgtg gagggagagg
    30181 cttgctcaaa tccgcgttca aggatttcca gattggtaag aacttcagat tccttgacgt
    30241 aatttggcgc accgtgggtg atggtttgat acaacgcatc ataaacgcgc ccgtaatcgc
    30301 ccatctccgg cttcatctct tctctgaccg tcacgccctc gtcattgaca tactccagca
    30361 caccgaccga atcatccgct gcgaatccct gttcgcccgg cataatatta gccttcaggc
    30421 tggtttcctg ctggtcgata ccgtatttaa taaacgaacc tttcttaccg tgaacgataa
    30481 atttcggata atcgattttc accagatggc tggttttgac gatggctttc aggtcgccat
    30541 aaaacagttg cgcttcaaag gtgtcgtcag gattggcttt attacgcagg ctgcggatgt
    30601 cataagcgac gtgatccggg cgaccgaaca gagaaataat ctggtccatc gtatgcacac
    30661 caaggccata aaacgcgcca tcctgcggca gcccaggttt ggtttctgcc accgggcggt
    30721 aatagtcaaa atggctttcc acttcaacaa tctctcccaa cttgccactt tcaatcgctt
    30781 ttttcgctgt caggaagcag gagtcaaagc gacgattctg atacggcgtg acggtcagcc
    30841 ctttgctttt cgccaacgca aacagctcct tcgcctgggc aagcgtcggg gtgaacggtt
    30901 tttcgaccag cacatttttc ccggcttcca gcgcgcgttt cgcgtactcg aaatggctgt
    30961 ccgcgtgggt gcagacaaca accagcttaa catcgggatc gtttagtact tcgtcgagat
    31021 cgctggtcaa atggatatgg gaataaatgg gagcctgttc ttccggcttc gcatggcgac
    31081 gaaaaatatg cgcgacatgc cagctatcct tgcggttaag tacatacggc agatggtaac
    31141 gggtggtgct tttgccgaag ccaataaagg cgcagttgat gaccatgatt tcgtcctttt
    31201 taaggtggtt atttacacct tagcgcaaag cggacgtggt tcctactgga gtgcgcataa
    31261 cgaacacaag cactcccgtg gataaattga gaacgaaaga tcaaaaaaaa agccagcacc
    31321 cggctggcta agtaatactg gaagcaatgt gagcaatgtc gtgctttcag gttctccgcg
    31381 agggtcttcc tgatcgcgag acaataataa tcattctcat tcgcacttgt ccaacacttt
    31441 ttgcaaaaaa atgcatttga ctcacatttg aaagtcaatg atgttgaaag ggacatttac
    31501 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
    31561 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
    31621 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
    31681 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcgccca
    31741 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
    31801 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
    31861 cgtatcgagc taacggtgtt cgtcgataac gcgccagcaa ttaaggtcta taaaaaattc
    31921 ggctttgaga ttgaagggac tggcaagaag tacgcattac gtaatggtga atatgtcgat
    31981 gcgtattata tggcgcggat gaaatagaga atttgcaatc taagtttgtc atttaagccc
    32041 tcacctaccg ggagagggct taagatatta gtaacccgcc gttaaatcat ccaccgagcg
    32101 cgggtcggat gcgccgtaca actcaccgtc cggcccaacc ataatgcttt gtgtactgcc
    32161 catcgcctct ttcagcgcca ctttctgacc ttttgcttcc agcagcttga gcgtatccgg
    32221 gctaaaccct ttttcgacac gcagctcgtc cggcaaccac tgatggtgga aacgcggcgc
    32281 attggtcgct tcggcgacgt tcatgccata atcgatgcta ttcaccacca tttgcagcac
    32341 tgtagtgatg atccggctac cgcctgggct accggtaacc agccaggttt taccgtcttt
    32401 caccacaatg gtcggcgaca tcgacgacag cgggcgtttg ttcggcccga cggcgttggc
    32461 atcaccgccc accagcccgt aaacgttcgg tacgcccggt ttggcggaga aatcatccat
    32521 ctggttatta agcagaatac cgctctcgcc cgcgacaata cccgtaccga aggtggtgtt
    32581 cagcgtatag gtcaccgcca ccgcgttacc atctttatcc accactgagt aatgggtagt
    32641 ttgattactc tcataaggcg caagcttgcc ggggcgaatt tcgctggatg gcttcgcttt
    32701 attgatatca atttgatcgg caatagattt ggcataggct ttattggtca gcgcctgcca
    32761 cggtactttg acaaaatccg ggtcgccaag atattccgag cggtcggcgt aggcgtattt
    32821 ctccgcttct gccatgattt gcatcgcatc ggcgctgcca aagccgtatt tcttcatatc
    32881 gaagttttcc agaatattga ggatttgtac gatatggatc ccgccggagg atggcggtgg
    32941 catggagtaa acctgatacc cgcgataatc gccgcttatc ggagtgcgtt cgaccgcttt
    33001 ataggccgct aaatcttctt tagtgatcaa gccaccgttt ttctgcatct cctgggcgat
    33061 ctgttccgca atcgtgcctt tatagaattc gtccgggccg ttttcagcaa tcatctccag
    33121 gctctttgcc aggttcgcct gcaccagcgt gtcgcccttt ttcagcggct cgccctcttt
    33181 ccagaagata gctttactgt tttcgtgatt cggcaacact tcgctaccgt aggttttgag
    33241 atcgtcagcc agcgcgtcgt taacgataaa accatcgcgt gccagtttaa acgcgggctg
    33301 cacgactttg ttcagcggca tggtgccgta tttatccagc gccagcgaga aacctgctac
    33361 cgtacccggt gtgccggaag ccagatgcga agtgagtgat tttttgctgt ccgggttgcc
    33421 ctgatcatcg aggaacatat cgcgggtcgc tttggcgggt gccatttcgc ggaaatcgat
    33481 agccgtggta ttgccatttt tcgagcggat taacataaaa ccaccaccgc ccagattccc
    33541 tgcctgcgga tgcgttaccg ccagcgcgta gcccaccgcc acggcggcat caacggcatt
    33601 cccgccctcc ttgaggatat ccacccccac ctgagtggca gtggcgtcca cagacgctac
    33661 cattccctgt ttcgcgcgta ccgggtggaa gacatcttcc tccacaccat acgagacggg
    33721 cggcgcagga ggcgcggcgg cggcgctaaa acaacttcct gagagcagag cagcaatggc
    33781 cacccggcgt aaaaacgtcg gttttatcat cgttattctc cagagattaa ggggcaaccc
    33841 cagctaagcc tggtatataa ctctgaatta atcatcgttt tgtcgggaag cgagtaaact
    33901 taaaggatat cttcagagga ggatacgcga tgaaacgact tctgattctt acggcactcc
    33961 tgccgtttgt cggctttgca cagcccatta atactctgaa caaccctaac cagccggggt
    34021 atcagatacc cagccagcag cggatgcaaa cccagatgca gactcagcaa atccagcaaa
    34081 aagggatgct gaatcagcaa ctgaaaacgc aaactcagtt gcaacagcag catttagaaa
    34141 accagataaa caataattct cagcgggtgt tgcagtcaca gccgggggag cgaaatcccg
    34201 cccggcagca aatgctgccc aacaccaacg gcgggatgtt aaacagcaac cgtaatccgg
    34261 atagttcgtt gaatcagcag catatgctgc cggagaggag aaacggcgac atgctgaatc
    34321 agcccagcac gccgcagcct gacattccgt tgaaaactat tgggccgtaa agttcggacc
    34381 aatcacgtca atcgcatcgg tacagatgca atccacgccc cagcgcagca actctgccgc
    34441 gcgctggggt ttattgacgg tataaaccag aatccgcagt ccggcgtctt tcaactgcat
    34501 cactcgcact ttatcgagta acttatgatt gagatgaata gagacgcagc ccagccgcgc
    34561 ggtcagttcg cgccagtcgt cgcgccactc atcaagcaat aaaccacgcg gcagttccgg
    34621 tgccgccagc tgtgcagctt ctaaagcatc aatctcaaac gatgacagca gcggcggcgt
    34681 cataccggcc cacagttggc gtgccgccag cgccaccatt ttgcccgtta atggtccagt
    34741 gccagtggtg ggtttgattt cgatattcgc catcatcccg tgttcgcgac agcgttccgc
    34801 cacctgcgaa agcaacggca gcggctcacc tttaaacgct ttgctgtacc aactgcccgc
    34861 atccacgcgc agtaaatcct gccagttcag ttcacccgcg acgccccagc cgttgctggt
    34921 gcgttcgaga ttgtcgtcat ggagcaggaa gatctcgcca tctttcgata acttcgcgtc
    34981 aaattcgatc atcttatgac cgtattttgc cccgacgtcg attgccgcca gggtgttttc
    35041 cggggccagc ttaccgccgc cacgatgagc gacgatgcgg ggataaggcc agttactcat
    35101 actcgttgtc ctgtttcacc atcaaaaaga tgcagctgat tttccgccag atgcagccac
    35161 agcgtgctgc ctgccgtcgg gcgctcctga tgcgccagtc gcaccaccag cttctgctcg
    35221 ccccagcgtc cgtgcgccag gttatctgcg ccgaggatct ccagcgtgtc catcaccagc
    35281 ggtacgccgc cttctgcctg cgagcttagc gcaatatgtt ccgggcgaat gccgagagtc
    35341 attttacgcc cggcatactg acggtagcca ccgtttagcg gcagctcaat accgccgtcc
    35401 agttcgaaat gcgtgccttc gttattcacg cggcctgtca gcaggttcat cgccgggctg
    35461 ccgataaaac tcgctacaaa caggctggcg ggcttttcgt agacttcaac tggtgtgcca
    35521 atctgttcgg caacgccgcc gttcatcacc attactcgct gggcgagcgt catcgcttca
    35581 acctgatcgt gagtaacgta gagtgaagtc gttttcaggc gacggtgcaa ctgttgcagt
    35641 tcaagacgca tctgcacgcg cagcttggca tcgaggttag agagcggctc atcaaacagg
    35701 aacaccgccg gatcgcgcac aatcgcgcgg cccatcgcca cacgctggcg ctgaccgccg
    35761 gaaagctcgc gcgggcgacg tttgagcaga ccgtccagct ccagaatgcg cgccgcttct
    35821 ttaacgcgct cggcaatttg ctgcttgccc atgccgcgaa ttttcagccc ccacgccatg
    35881 ttttcttcga cactcatatg cggataaagc gcgtagttct ggaacaccat cgcaatcccg
    35941 cgatcttttg gctccatttc agtcacgcgc tggtcgttga tccagatatc gccttctgtc
    36001 acccgctcca gcccggcaac catgcgcagc agcgtcgatt tcccgcagcc agacggcccg
    36061 accatcacga taaattcgcc atccgccaca tcaagggtca gcggtttaat gacctgggtt
    36121 ttaccatccc agcttttggt tactgcctgt aatttcagtc ctgccatctt atttctcact
    36181 atcgaccagg ccgcgcacga aggcacgctg catcactaaa acaatcacca ccggagggat
    36241 aagcgttaac aacatcgccg ccatcactga gttccattca gtggtgcctt cgcctgtagc
    36301 gatcatccct ttgatccctg ccacggtggt gccgagatcc acatcggtaa taatcaacaa
    36361 cggccacaaa tactggttcc agccgtaaat aaaggtgatc acaaacagcg ccgccagatt
    36421 ggttttggag agcggaaaaa cgatgtcgca aaagaaacgc attggtgacg cgccgtcgat
    36481 ccgcgccgct tccaccagtt catccggcag cgtcataaag aactggcgaa acagaaacgt
    36541 ggcggtcgcc gaggccatca gcggcagcgt taaaccggcg tagctgtcga gcatcttcag
    36601 gttggcgatg acttccaccg tcgggaagat acgtacttca accggcagca tcagggtgat
    36661 aaaaatcatc cagaagaaga ggttacgtag cggaaaacga aaccagacaa tggcaaatgc
    36721 cgagagcatc gagacggtaa ttttgccgag cgtaatgctg aacgccatca caaagctgtt
    36781 aagcaacatc cgccagaacg gcgcgctatt cgtgcctacc ccgttcaccc agatgttgtg
    36841 gatgttttcc agcagatgtg tgccggggat gagcgtcatc ggcgcggcat agacggcctg
    36901 tttatccagc gtcgccgcga caaacgccac gtacagcggg aagaggatca ccgcgatccc
    36961 gaggatcagc atggtatggc tgaatatcgt cagccacgga cggttctcaa tcattggtaa
    37021 cgcaccttgc tttcaacata gcggaactgc accaccgtca gcacgatgac gaggaacatc
    37081 aacaccaccg actgtgcggc agacgaagcc agatccagtc cggtaaaacc ttcgcggtag
    37141 atcttataaa tcagcgtcgt ggtggcctga accggcccgc cggacgtggc ggcgtcgatc
    37201 accgggaagg tgtcgaagaa ggcatacacc aggttcacta ccagcaggaa gaaactcacc
    37261 ggggcgataa gcggcagcgc aatcttaaag aagcggcgaa tcggccctgc accgtcgatg
    37321 gctgcggctt cgatcaacga acggggaatg gattgcagcg cggcatagaa gaacaggaag
    37381 ttgtagctga tttgcttcca tactgaggca aacaccacca gaaacattgc ctgaccgctg
    37441 ttttgcgcgt ggttccagtc gtagccgaac tccgcgagaa aatgggtgat cagcccgcga
    37501 ccggggttaa acaggaagat ccacaatacg gcggcaacgg cgggagccac ggcgtaaggc
    37561 agcaacatta aggtttgata gaaacggctg ccgcgcacga tgtactccac cagcgccgcg
    37621 aagaacagcg acaccagcaa accgctgacg gtgacaaagg tgctgaattt tatcgtcgtc
    37681 cagaaggagt cgagatagta gctgtcatga aacagcgcga cgaagttatc caggccgaca
    37741 aactggctgg agaaaccaaa cggatcgacg ctttgtagcg agtaccacaa cgcttcgccc
    37801 gcaggccaga taaaaaagat aacggtgatg atgagctgcg gcgcgaccag cagataaggc
    37861 agccagcgcg agcggaacac cggacgggat gatgacatta cggttaattc ctgaactgtg
    37921 ccggatgcgc ttcgcttatc cggcctacac gcgatgccag gtcagataag cgaaacgcat
    37981 ccgacattac actgattaag acttcgtcga tttctcaaag cggcgcagca actgatttcc
    38041 acgctcaacg gcggtatcca gtgcctgctg tggtgtcttc ttaccggtcc acacgctctc
    38101 cagctcttca tccacaatca cgcggatctg cggcatgttg cccagacgca gccctttggt
    38161 gaacggcaac ggcggcttgt tcagcatctg acgcgtcgcg gtatccgccc ctgggttttt
    38221 ctcataaaag ccctgctcac gggtcaggtc atacgctgct ttggtgattg gcagataacc
    38281 ggttttctga tgccactcgg cagcgttttc tggcttcgcg aggaagtcga ggaacttcgc
    38341 cacgccggta tacgtttctt tatttttgcc ctgcatcacc cacaggctgg ccccgccgat
    38401 aatggcgttt tgcggtgcat ctttcgcatc ggcgtcgtaa ggcatcatgc ctacgccata
    38461 gttaaatttg gcgtactcac gaatattagc gagagaaccg gaagaggcgg tggtcatcgc
    38521 gcaatcaccg ttatagaact tctcggtgga ttcatcctta cgaccgacgt agctgaagtc
    38581 gcccttcttg ttcatctcct cgagcatggc gatgtgtttc acctgctccg gcttgttgaa
    38641 ctccagcacc gcgtccgtgc cgtcaaagcc gttgtttttg ctggcaaacg gcagaccgtt
    38701 ccaggcgcta aagttttcca gttggatcca gccctgccag ccgctggcgt agccgcactt
    38761 cataccggag gctttcagtt tcgcggcata gtccgccaga tcctgccagg ttttcggcgg
    38821 ttgttccggg tctaatcctg ctttcttgaa ggcgtctttg ttgtaataga gaacgggggt
    38881 cgagctgttg aatggctggg agagtaagtg gcccgttttg ctgtcggagt agtaaccgga
    38941 aaccgtcggc acaaactgcg actcatcgaa ttgaatcccc gcctctttaa acacgtcata
    39001 taccggttta atggctttcg atgccatcat ggtggcggtg ccaacttcat aaacctgcaa
    39061 aatagccgga gcgttgccgg tacgaaatgc ggcaatcccc gcgcttaaat tctgttcgta
    39121 gttgccttta taggtcggta caattttgta atccggattt tcggcgttaa aacgttgggc
    39181 cagagaatcc acctctttac ccagttcccc ttccatagaa tgccagaacg gaatggtcgt
    39241 cactgcctgt gcattcccca ttaacgccag tccgagcgcc agtgctgaag ctgtataatg
    39301 taacggtttc atcgtttatc tctcttgttg taccgaatgc gcgaattcac gcgttttatg
    39361 ctcgcggggt aacatgacat gctcgaatta cagaaaaata acttttttgt tacatttgta
    39421 agatagtaag gtgtcagaaa gatgacaagg cggtgacggc gtgggtgagg gaaaatggga
    39481 gatggggcac ggataagcgg aaaatagaag gtctgaatca aactctacag attactcatc
    39541 gtttcatgcc ggatgcggcg taaacgcctt atccggccta caagatcgtg caaattcaat
    39601 atattgcaac tcacccagta ggcctgataa gcgcagcgca tcaggcaatt ttacatttgt
    39661 cacctgtctc aaaggagtct tttgactcct tatcaatcaa cgtgttatta cccgcctaaa
    39721 tacgcacttc tcactgcttc gttcgccagc agcgcgtcgc cggtatcgga gagcaccaca
    39781 tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt ggcgttctgc
    39841 tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc gaaaatttgc
    39901 tggatgataa tcggcgcaag accgagcgat ggctcatcaa gtagcagcaa acgcgggttg
    39961 ctcatcagcg cgcgaccaat cgccagcatt tgttgctcac cgccggacat ggtgcctgcc
    40021 cgctgaacac ggcgctcatg cagacgtgga aacagttcat acacccactt tatgcgctcc
    40081 tggaactggt cgcgttcggc aaaaaagccg cccatcgcca ggttctcttc caccgtcatc
    40141 cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat gattttcgcc
    40201 gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt agcgcgcgga
    40261 tcgccgcata acgtgccgag caatgtggtt ttccccgccc cgttcgcgcc aatgagggtg
    40321 acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat tttgccgtag
    40381 tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct cacctaaata
    40441 ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg ccagtggcgt
    40501 cccctgattg accacataaa ttcggtctga aatccccatc accagcttca tatcgtgttc
    40561 aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct catccagctc
    40621 tttggtttct tttgggttaa gacctgccgc cggttcgtcg agcattaaaa tctccggctg
    40681 cgtcaccatg cagcgggcaa tctccagacg gcgttggtca ccataggcca ggttactcgc
    40741 ctgacggttg gcgtgttcca gcaaaccaat gcgctcaagc caggtggcgg cgcggtcgag
    40801 cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga acagcccggt
    40861 tttcagttgt tgatgctgcg ccaccagcag gttttcaatc accgtcattt cacggaacag
    40921 acgcacatgc tggaaggtac gcaccacgcc catgcgggca atttgctgcc ccggtaaacc
    40981 ttccagatgc tgatcacgca gtaaaatagt gccgccggtg ggtttgtaga atccggtcag
    41041 gcagttaaaa accgtggttt ttccggcacc gttagggccg attaacgaga cgatctcctg
    41101 cgggtacagt tcaagattga cgttgttcac cgccagcaag ccgccgaagc gcatcatcag
    41161 accgttaaca gataataatg gctgactcat gcctgctctc ctttcgctgc gccgtttttc
    41221 agcttcagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat catcatcagc
    41281 accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat caattcgcgc
    41341 gataccacca gcaaaattgc cgccagaatc accgcaaact gcgatcccat accgccgagc
    41401 accactatcg ccagtacaaa cgccgattca gcaaaggtga aggattccgg gctgacaaag
    41461 ccctggcgcg cagcaaacag cgttccggca aaaccggcaa acgcggcact tatggtaaag
    41521 gcagtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat ctcgtcttca
    41581 cgcaacgctt cccacgcacg ccccagcggc atccgcagca ggcggttaat gacaaacagg
    41641 cttagcacca ccagcagcaa cgccaccagg tagaggaaga tgacacgatc ggagggatcg
    41701 tatttcaggc caaagaaatt actgaacgtg tcccagccgc cttcacgagc ggtacggctg
    41761 aactcgagtc cgaagagtgt cggtttcggg atctgactga ttccgttcgg gccgccggta
    41821 atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag ggtaacgatc
    41881 gccagatagt cgccgcgcaa gcgaagcacc gggaagccga gcaggaagcc ggccgccgct
    41941 gccattaacc cggcaatcgg cagacaggtc cagaagccca ggccgtaata gtgattgagc
    42001 agcgcaaaag tgtaagcgcc gatggcgtaa aagccgccgt accccagcac cagcagacca
    42061 gaaagaccaa caaccacgtt cagcccgagg ccgaggataa tgtagatcat ggttagggtg
    42121 gcgatatcca ccgtcccacg cgacaccata aacggccacg ccaccgcaag caccagcagc
    42181 gccacgagga atagtttctg cttcaccgtg gagccatcaa tcgcgggcag aataaacttc
    42241 ggtccggaaa cgctttttag ccctttctgg aaagccggtc gcaaaagctg gaagaaaaag
    42301 accaccgccg tgccgataaa cacccactgc caacggatgt ctgaagccgt gtcgaccacc
    42361 agtttggtgc catccagctc cagttgcacg cccataaaga cgcccgccag gacaaaaaac
    42421 atcgcggcag agagcagcgc cattgcaata tgcatcggtt tcatactttc tctacctccg
    42481 ggcgacccag aataccggtc ggcatcacca gcagcaccag aatcagcagg gcgaatgaga
    42541 ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca atccccagaa
    42601 tcagcccgcc aatcatcgct cccggaatgc tgccaatccc accgagcacc gcagcggtaa
    42661 aggctttcat cccggccata aagccgatgt aggggttaat tacgccgtag aactgaccga
    42721 gcagcacacc tgccaccgcc gccatcgccg cgccaatcac aaaggtcagc gcaatcaccc
    42781 ggtcggtgtt aatgccaagc aggctcgcca ttttcaggtc ttccgcgcag gcacgacacg
    42841 cgcgccccat acgggaatag cgaatgaaaa ccgtcagcgc cagcatggcg aggaaggtga
    42901 caatccagat caccgcctgc atggtggtaa tagaggcaga gaagttttcg ctatgcccca
    42961 ccacccactg accgttaaac agactgggca gcgccacgtc acgcgaacct tcagtcaggc
    43021 taacgtagtt ttgcaggaag atggacatgc cgattgccga gatgagcgca atcaggcgct
    43081 tagagttacg caccgggcga taggctaccc gttcgatact ccagccgtag gcgctggcga
    43141 tgacgattgc gccgacgaat cccgccgcca ccagcagcca gccggtatca atgcccatca
    43201 tcatcagcgc ggcgatgatc ataaatgaga catagctgcc gatcatataa acctcgccgt
    43261 gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg gcaatcagcg
    43321 cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat aaaaactgct
    43381 cagacataag gtaacctttc taaacccgcc cgtttttcac gggcggtggg atgatcactt
    43441 ggctgccgtg gatgaaccgt cggcgtgcca ctggaagaca ccaaaatcaa atcccttaag
    43501 atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt tagcttttaa
    43561 atctttcacc agcgccagcg gctcatcgct gccagtacgc tcaagggcag tcgccagaga
    43621 ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct tgtctgcttt
    43681 cagcgcatca acgatgccct ggtttgccgg atcctggtca tagcgttttg gcatagtgac
    43741 caacatgcct tcggcagcat cgccagcaat attcgacaat gatgcgttac ccacaccttc
    43801 cggccccata aactgagttt tcaggccaac ggaacgggcc tggcgcagca tctgccccat
    43861 ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca ggcgggcgat
    43921 cagcgcggag aaatctttct ccccggcggt aataccgtcg aagaagacga cgttggcgtt
    43981 agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact gttgtttgtc
    44041 gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg ccgccgtagg
    44101 cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc gttgggtcag
    44161 ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcgt agatatctga
    44221 cgcaggttgg gtagaagaag aacacagatg accaataacg tatttaatgc cgtcgttaac
    44281 gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat attccacgcc
    44341 caccagctta tcgcctttaa ttcccccttt ggcattaatg tctttaatcg cctgacgcgc
    44401 gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc cgacaacggc
    44461 aactttaata tcgtcagcca tagcggtgtg tgaaattgcc agtgcaatca tccctgcgat
    44521 gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt gtgttgcttt
    44581 gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt tttgcctgtc
    44641 tgattaaggg tttagcgcag tattttgtga taatagcgat taaaatccct atttttcagt
    44701 cgattaagaa cagataatat tctgaattta ttgatagata aacagaaaaa agcgccgttg
    44761 tcagcataaa ataacggtac aaagagcgga ataa
//
LOCUS       DAPHJE010000024        44731 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPHJE010000024 DAPHJE010000000
VERSION     DAPHJE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.024"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(84..158)
                     /gene="tnaC"
                     /locus_tag="RH222_001345"
     CDS             complement(84..158)
                     /gene="tnaC"
                     /locus_tag="RH222_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDV3796397.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(401..1765)
                     /gene="mnmE"
                     /locus_tag="RH222_001346"
     CDS             complement(401..1765)
                     /gene="mnmE"
                     /locus_tag="RH222_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV3796398.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1871..3517)
                     /gene="yidC"
                     /locus_tag="RH222_001347"
     CDS             complement(1871..3517)
                     /gene="yidC"
                     /locus_tag="RH222_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV3796399.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3520..3777)
                     /gene="yidD"
                     /locus_tag="RH222_001348"
     CDS             complement(3520..3777)
                     /gene="yidD"
                     /locus_tag="RH222_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV3796400.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3741..4100)
                     /gene="rnpA"
                     /locus_tag="RH222_001349"
     CDS             complement(3741..4100)
                     /gene="rnpA"
                     /locus_tag="RH222_001349"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV3796401.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4117..4257)
                     /gene="rpmH"
                     /locus_tag="RH222_001350"
     CDS             complement(4117..4257)
                     /gene="rpmH"
                     /locus_tag="RH222_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV3796402.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4487..4567)
                     /locus_tag="RH222_001351"
     CDS             complement(4487..4567)
                     /locus_tag="RH222_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796403.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4864..6267
                     /gene="dnaA"
                     /locus_tag="RH222_001352"
     CDS             4864..6267
                     /gene="dnaA"
                     /locus_tag="RH222_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV3796404.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6272..7372
                     /gene="dnaN"
                     /locus_tag="RH222_001353"
     CDS             6272..7372
                     /gene="dnaN"
                     /locus_tag="RH222_001353"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV3796405.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7372..8445
                     /gene="recF"
                     /locus_tag="RH222_001354"
     CDS             7372..8445
                     /gene="recF"
                     /locus_tag="RH222_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV3796406.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8474..10888
                     /gene="gyrB"
                     /locus_tag="RH222_001355"
     CDS             8474..10888
                     /gene="gyrB"
                     /locus_tag="RH222_001355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV3796407.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            11128..11526
                     /locus_tag="RH222_001356"
     CDS             11128..11526
                     /locus_tag="RH222_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV3796408.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11641..12453
                     /gene="yidA"
                     /locus_tag="RH222_001357"
     CDS             11641..12453
                     /gene="yidA"
                     /locus_tag="RH222_001357"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV3796409.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12499..13155)
                     /locus_tag="RH222_001358"
     CDS             complement(12499..13155)
                     /locus_tag="RH222_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796410.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13433..14122
                     /gene="dgoR"
                     /locus_tag="RH222_001359"
     CDS             13433..14122
                     /gene="dgoR"
                     /locus_tag="RH222_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDV3796411.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14119..14997
                     /gene="dgoK"
                     /locus_tag="RH222_001360"
                     /pseudo
     CDS             14119..14997
                     /gene="dgoK"
                     /locus_tag="RH222_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            14981..15598
                     /gene="dgoA"
                     /locus_tag="RH222_001361"
     CDS             14981..15598
                     /gene="dgoA"
                     /locus_tag="RH222_001361"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV3796412.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15595..16743
                     /gene="dgoD"
                     /locus_tag="RH222_001362"
     CDS             15595..16743
                     /gene="dgoD"
                     /locus_tag="RH222_001362"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDV3796413.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16818..18155
                     /locus_tag="RH222_001363"
     CDS             16818..18155
                     /locus_tag="RH222_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796414.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(18152..19216)
                     /gene="cbrA"
                     /locus_tag="RH222_001364"
     CDS             complement(18152..19216)
                     /gene="cbrA"
                     /locus_tag="RH222_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV3796415.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            19317..20531
                     /locus_tag="RH222_001365"
     CDS             19317..20531
                     /locus_tag="RH222_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV3796416.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20533..20865)
                     /locus_tag="RH222_001366"
     CDS             complement(20533..20865)
                     /locus_tag="RH222_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV3796417.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    21105..21177
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            21171..21584
                     /gene="ibpA"
                     /locus_tag="RH222_001367"
     CDS             21171..21584
                     /gene="ibpA"
                     /locus_tag="RH222_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV3796418.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21696..22124
                     /gene="ibpB"
                     /locus_tag="RH222_001368"
     CDS             21696..22124
                     /gene="ibpB"
                     /locus_tag="RH222_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV3796419.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22321..23982
                     /locus_tag="RH222_001369"
     CDS             22321..23982
                     /locus_tag="RH222_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV3796420.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23979..24695)
                     /locus_tag="RH222_001370"
     CDS             complement(23979..24695)
                     /locus_tag="RH222_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3796421.1"
                     /translation="MIYKSIAERLRIRPNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24991..26607
                     /locus_tag="RH222_001371"
     CDS             24991..26607
                     /locus_tag="RH222_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3796422.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26607..27245
                     /locus_tag="RH222_001372"
     CDS             26607..27245
                     /locus_tag="RH222_001372"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796423.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQERFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            27245..27421
                     /locus_tag="RH222_001373"
                     /pseudo
     CDS             27245..27421
                     /locus_tag="RH222_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(27418..28311)
                     /locus_tag="RH222_001374"
     CDS             complement(27418..28311)
                     /locus_tag="RH222_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3796424.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28478..30193
                     /locus_tag="RH222_001375"
     CDS             28478..30193
                     /locus_tag="RH222_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV3796425.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSIGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            30190..31683
                     /locus_tag="RH222_001376"
     CDS             30190..31683
                     /locus_tag="RH222_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV3796426.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31730..32179)
                     /locus_tag="RH222_001377"
     CDS             complement(31730..32179)
                     /locus_tag="RH222_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796427.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            32289..32636
                     /locus_tag="RH222_001378"
     CDS             32289..32636
                     /locus_tag="RH222_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV3796428.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32626..32988
                     /locus_tag="RH222_001379"
     CDS             32626..32988
                     /locus_tag="RH222_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV3796429.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32985..33482
                     /locus_tag="RH222_001380"
     CDS             32985..33482
                     /locus_tag="RH222_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV3796430.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33490..34674)
                     /gene="emrD"
                     /locus_tag="RH222_001381"
     CDS             complement(33490..34674)
                     /gene="emrD"
                     /locus_tag="RH222_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV3796431.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(35093..35182)
                     /gene="tisB"
                     /locus_tag="RH222_001382"
     CDS             complement(35093..35182)
                     /gene="tisB"
                     /locus_tag="RH222_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV3796432.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35747..35845
                     /gene="ivbL"
                     /locus_tag="RH222_001383"
     CDS             35747..35845
                     /gene="ivbL"
                     /locus_tag="RH222_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV3796433.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35951..37639
                     /gene="ilvB"
                     /locus_tag="RH222_001384"
     CDS             35951..37639
                     /gene="ilvB"
                     /locus_tag="RH222_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV3796434.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37643..37933
                     /gene="ilvN"
                     /locus_tag="RH222_001385"
     CDS             37643..37933
                     /gene="ilvN"
                     /locus_tag="RH222_001385"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV3796435.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            38008..38598
                     /gene="uhpA"
                     /locus_tag="RH222_001386"
     CDS             38008..38598
                     /gene="uhpA"
                     /locus_tag="RH222_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV3796436.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38598..40100
                     /gene="uhpB"
                     /locus_tag="RH222_001387"
     CDS             38598..40100
                     /gene="uhpB"
                     /locus_tag="RH222_001387"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV3796437.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            40110..41429
                     /gene="uhpC"
                     /locus_tag="RH222_001388"
     CDS             40110..41429
                     /gene="uhpC"
                     /locus_tag="RH222_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV3796438.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            41567..42958
                     /gene="uhpT"
                     /locus_tag="RH222_001389"
     CDS             41567..42958
                     /gene="uhpT"
                     /locus_tag="RH222_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV3796439.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(43003..>44731)
                     /gene="adeD"
                     /locus_tag="RH222_001390"
     CDS             complement(43003..>44731)
                     /gene="adeD"
                     /locus_tag="RH222_001390"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV3796440.1"
                     /translation="RAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGR
                     YIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTV
                     ICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLA
                     WRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGI
                     ENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPW
                     EIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLS
                     DARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPG
                     KRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLN
                     EGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGL
                     MSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKF
                     AFTTLEVTE"
BASE COUNT        10328 a        11462 c        12132 g        10809 t
ORIGIN      
        1 aaggcaaaat taagatatag caaacaaata gatcacattg aatctaatcg gtttggctct
       61 ggtgatggct acagaagggc aaattaaggg cggtgatcga caatcttatt gtcgatattg
      121 aaccattttg aggtcacaca tatatgtaag atattcataa tgcacttatc ctcgaaagac
      181 acgaggctac agtataatac tcaagaattt tgtctattct aaaattgttt aaatgtgaat
      241 cgaatcacaa tcgttcgggg agcaatatta aatgcttttt tttaagatta taaattacgt
      301 aagaatttta atgtttaaaa aaagggttga ggaataacag gagtgctaac aatgaagcga
      361 agccgcatct gacagtcaga atacggcttc gtaagcgcgg ttacttacca atacagaagc
      421 tggagaaaat ccgccccagc aggtcgtctg aagtaaattc cccggtgatt tcgcttaagt
      481 tctgctgtgc cagacgcaac tcttccgcca gcagttcacc tgcccaggct cccaacagtt
      541 gcgctttgcc ctgttgtaga tgttccgctg cctgttccag cgcctgtagg tggcgacgac
      601 gcgccaggaa gccgccttcc atgttggtgt caaagcccat gctctgtttg agatggttac
      661 gcagcacgtc cacgccttca ccagtccttg ccgagagacg aattaacgcg tgaccgttca
      721 cttcactcat tcccagcgtt tcgccggtga tatcggcttt attgcgcacc acggtgatcg
      781 gcagtttcgc tggcagacgg gcaataaatt ccggccagat ctctgccgga tccacggcgt
      841 ctgttgtggt gccatcgacc ataaacagca cgcggtcggc ctgttcaatt tcctgccacg
      901 cgcgctcgat accaatacgt tctacttcgt cactggcttc acgtagcccg gcggtatcga
      961 tgatatgcag cggcattccg tcaatgtgga tatgctcacg cagcacgtca cgcgtagttc
     1021 cggcgatatc ggttacgatt gccgcttcac gccccgccag cgcgtttaac aggctcgatt
     1081 taccggcgtt aggacgtccg gcaatcacca ctttcatccc ttcgcgcaac aaactaccct
     1141 gacgtgcttc agcacgcact gcatcaagat cggcaataac gtcattgagc tgggcttcaa
     1201 tttttccgtc ggagaggaaa tcgatctctt catcggggaa atcaattgcc gcttcgacgt
     1261 aaatgcgcaa gtgggtgagg gcttctacca gatgattaac ccgtgcggag aatgcgcctt
     1321 gcagcgagtt aagtgccgaa cgggccgcct gttccgaact ggcgtcgata agatcggcaa
     1381 tcgcctcggc ctgggctaag tcaagtttat cgttaagaaa cgcgcgttcg gaaaactcac
     1441 cagggcgagc aatccgcagg ccgggaatgg tcagaatgcg ttttaacagc aggtcgagga
     1501 tcaccggacc gccatgacct tgcagttcca gcacatcttc gccggtgaac gagttcgggc
     1561 cagggaacca tagcgcaatc ccctgatcga gcacgctgcc gtcggcgtct ttaaacggaa
     1621 gataatcggc gtagcgcggc ttaggcagtt tacccagcac ggtttcggca acttcacggg
     1681 ctttgaagcc ggagatgcgc aggatgccaa cgccgccacg tcccggaggc gtggcctggg
     1741 ctacgatagt gtcattatcg ctcatgatgt tcctgttgct ttgtgtggcg gatgcgcggt
     1801 gcttatccgc cctacgaaaa gaaaaaaggc ggtcaactga ccgcccttat tttagcgaaa
     1861 actcaccgaa tcaggatttt ttcttctcgc ggctatgcag gccacgtttt tccagaccac
     1921 ggtaaatcag ctgctgctga ataatggtta ccaggttgct gacgatatag tacagcacca
     1981 gacctgacgg gaaccacagg aagaacacgg tgaagatgac cggcataaag gtcatgatct
     2041 tctgctgcat cgggtcggtc actgtggtcg gcgacatctt ctgaatgaag aacatcgtta
     2101 cgcccatcag gatcggcagg atgtagtacg ggtcctgtgc cgacaggtcg tggatccaca
     2161 gtgcaaacgg tgcctgacgc agttcaacgg aacccatcag catgtagtac aacgccagga
     2221 agattggcat ctggatcagc agcgggaagc agccgcccag cgggttaacc ttctcagctt
     2281 tgtacagcgc catcatttcc tggctgatac gctgtttgtc atcgcccaga cgctcacgca
     2341 ttgcctgaat cttcggctgc agcatacgca tcttcgccat ggaggtgtac tgcgctttgg
     2401 tcagcgggta catgatgcca cgaacgataa aggtgatgat gataatggag aagccccagt
     2461 tacccacaaa gctatggatc catttcagca gtttgaacag cggctgagag atgaaccaca
     2521 accaaccgta atcaacggtc agatccaggt gcggagcaac agctgccatt ttgtcctgga
     2581 tttccgggcc aacccacagg gtgctgttca tcgcgccagt ctgaccaggc tgaaccagta
     2641 ccggctgaga tttatagccg atagcggcga tgccgttacc cagattagcg gtatagaagt
     2701 tgttggtacc gtcgttatgc gggatccacg ccgtcgcgaa atactgttgc agcatcgcca
     2761 cccaaccacc tttcgaagag atgttcaggt tttcgttatc ggcaatggta tcgaacttgt
     2821 atttctcata cttctcgtca ggcgtggagt acgccgcgcc acggaaggtg tgcagtgcga
     2881 agttgctgct tccggtatcg agatgcggtg gcagagtgat ggattgcttc aactgaccaa
     2941 aggtggagat ttccagcggt ttctcgccag cgttctgcac gttgtagttg acgttgacag
     3001 cgtaatcacc acgtttcagg acaaacgttt tggtaaacgt gttgcctgcc gcgtcggtat
     3061 acgtcatcgg cacctgcagt tcgttttgac cttcagccag cacataagcg tctttttcaa
     3121 cgttatacag cggacgcggg ccgttagccg ggttatccgg gccatcacga ccggtcagac
     3181 cgctctgtgc ctgataaata aactgcggtg aagtttccag cagctggaac ggctgggtag
     3241 agttcagctc tttcgggtaa gccggcagca gagcttgctc aacatcacca ccacgggtgt
     3301 tgatggtcag atcaagcacg tcggtcttaa ccgagatcag tttcccctgg ccactggccg
     3361 gtacgccctg gtcggcggcg ctacccgctg cggtggtcgt tgtctgcgtg gtctgttggg
     3421 cctgaggttg cgggttttta tcctgctccc aggcttgcca gatcatgaaa gacacgaaca
     3481 gcaaagcgat gactaaaaga ttgcgttgcg aatccatcgt tagtgttctc tggtatcaaa
     3541 tggtccgggc gggacgggat cgtcaccacc agggtgtaaa gggtggcatt ttaatacgcg
     3601 tttcaccgtc aaccaactgc cttttatcac tccaaacctg cgcaatgcct caattccgta
     3661 gcttgaacag gttggagtga aacgacaatg cggcccgagt agcggactaa tcaggcgttg
     3721 atagacccga atgagggcta tcaggacccg cgagccaggc gacagtggcg gcgccataat
     3781 ttttccaacg cttccgagag agcacggtta tcgaggtcgg caaccccttt tttcgccacc
     3841 accacgaaat ccatagccgg gagttcatgt tggcgcagac ggaagctttc acgcgtcaga
     3901 cgtttaatcc gattgcgttc atgggcgcgt cgaacgtttt tcttggcgac tgtaagaccg
     3961 atacggggat gccccagcga attcaggcgg ccgagaatgg taatttgcgg cgtgccagcc
     4021 cgttgtggct gctggaagac gaatgtgaat tgactgggag ttaacaagcg taactccctg
     4081 ggaaatgcga gcttaaccac tcaggggtta gctttattac ttagaaacgg tcagacgagc
     4141 gcggccttta gcacgacgac gtgccagaac ctgacgacca tttttagtag ccatacgagc
     4201 acggaagccg tgagaacggt tgcgcttcag tacagacggt tgaaaagtgc gtttcatggc
     4261 gatttctacc taaacttgaa taaattcaat ggctttattg gatatccgcc gaaaaatgaa
     4321 acgatggaca ccgaagccat gggtgattaa agaggccgga ttgtaataat tgtacactcc
     4381 ggagtcaatt ctctttcctt atttaccgcg cttttccgca ccttttcgca gggaaaatgt
     4441 acgacctcac accagtgaaa accagcatgg cgcgccgggt ggaggattat acgggctgat
     4501 gggtaaagcg caaggatcgt cctggatctt tattagatcg attaagccaa tttttgtcta
     4561 tggtcattaa attttccaat atgcggcgta aatcgtgccc gcctcgcggc aggatcgttt
     4621 acacttagcg aggtctggaa agtcctgtgg ataaatcggg aaaatctgtg agaaacagaa
     4681 gatctcttgc gcagtttagg ctatgatccg cggtcccgat cgttttgcag gatcttgatc
     4741 gggcatataa ccgcagacag cggttcgtgc gtcaccctca agcagggtct tttcgacgta
     4801 cgtcaacaat catgaatgtt tcagccttag tcattatcga cttttgttcg agtggagtcc
     4861 gccgtgtcac tttcgctttg gcagcagtgt cttgcccgat tgcaggatga gttaccagcc
     4921 acagaattca gtatgtggat acgcccattg caggcggaac tgagcgataa cacgctggcc
     4981 ctgtacgcgc caaaccgttt tgtcctcgat tgggtacggg acaagtacct taataatatc
     5041 aatggactgc taaccagttt ctgcggagcg gatgccccac agctgcgttt tgaagtcggc
     5101 accaaaccgg tgacgcaaac gccacaagcg gcagtgacga gcaacgtcgc ggcccctgca
     5161 caggtggcgc aaacgcagcc gcaacgtgct gcgccttcta cgcgctcagg ttgggataac
     5221 gtcccggccc cggcagaacc gacctatcgt tctaacgtaa acgtcaaaca cacgtttgat
     5281 aacttcgttg aaggtaaatc taaccaactg gcgcgcgcgg cggctcgcca ggtggcggat
     5341 aaccctggcg gtgcctataa cccgttgttc ctttatggcg gcacgggtct gggtaaaact
     5401 cacctgctgc atgcggtggg taacggcatt atggcgcgca agccgaatgc caaagtggtt
     5461 tatatgcact ccgagcgctt tgttcaggac atggttaaag ccctgcaaaa caacgcgatc
     5521 gaagagttta aacgctacta ccgttccgta gatgcactgc tgatcgacga tattcagttt
     5581 tttgctaata aagaacgatc tcaggaagag tttttccaca ccttcaacgc cctgctggaa
     5641 ggtaatcaac agatcattct cacctcggat cgctatccga aagagatcaa cggcgttgag
     5701 gatcgtttga aatcccgctt cggttgggga ctgactgtgg cgatcgaacc gccagagctg
     5761 gaaacccgtg tggcgatcct gatgaaaaag gccgacgaaa acgacattcg tttgccgggt
     5821 gaagtggcgt tctttatcgc caagcgtcta cgatctaacg tacgtgagct ggaaggggcg
     5881 ctgaaccgcg tcattgccaa cgccaacttt accggaaggg cgatcaccat cgacttcgtg
     5941 cgtgaggcgc tgcgcgactt gctggcattg caggaaaaac tggtcaccat cgacaatatt
     6001 cagaagacgg tggcggagta ctacaagatc aaagttgcgg atctcctttc caagcgtcga
     6061 tcccgctcgg tggcgcgtcc gcgccagatg gcgatggcgc tggcgaaaga gctgactaac
     6121 cacagtctgc cggagattgg cgatgcgttt ggtggtcgtg accacacgac ggtgcttcat
     6181 gcctgccgta agatcgagca gttgcgtgaa gagagccacg atatcaaaga agatttttca
     6241 aatttaatca gaacattgtc atcgtaaacc tatgaaattt accgtagaac gtgagcattt
     6301 attaaaaccg ctacaacagg tgagcggtcc gttaggtggt cgtcctacgc taccgattct
     6361 cggtaatctg ctgttacagg ttgctgacgg tacgttgtcg ctgaccggta ctgatctcga
     6421 gatggaaatg gtggcacgtg ttgcgctggt tcagccacac gagccaggag cgacgaccgt
     6481 tccggcgcgc aaattctttg atatctgccg tggtctgcct gaaggcgcgg aaattgccgt
     6541 gcagctggaa ggtgaacgga tgctggtacg ctccgggcgt agccgttttt cgctgtctac
     6601 cctgccagcg gcggatttcc cgaacctcga tgactggcag agtgaagtcg aatttaccct
     6661 gccgcaggca acgatgaagc gtctgattga agcgacccag ttttctatgg cgcatcagga
     6721 cgttcgctat tacttaaatg gtatgctgtt tgaaaccgaa ggtgaagaac tgcgcaccgt
     6781 ggcaaccgac ggccaccgtc tggcggtctg ttcaatgcca attggtcaat ctttgccaag
     6841 ccattcggtg atcgtaccgc gtaaaggcgt gattgaactg atgcgtatgc tcgacggcgg
     6901 cgacaatccg ctgcgcgtac agattggcag caacaacatt cgcgcccacg ttggcgactt
     6961 tatcttcacc tccaaactgg tggatggtcg cttcccggat tatcgccgcg ttctgccgaa
     7021 gaacccggac aaacatctgg aagctggctg cgatctgctc aagcaggcgt ttgctcgcgc
     7081 ggcgattctc tctaacgaga aattccgcgg cgtacgtctt tatgtcagcg aaaaccagct
     7141 gaaaatcacc gccaacaacc cggaacagga agaagcggaa gagatcctcg acgttaccta
     7201 tagtggtgcg gagatggaaa tcggcttcaa cgtcagctac gtgctggatg ttctgaacgc
     7261 gctgaaatgt gaaaacgtcc gcatgatgct gaccgattcg gtttccagcg tgcagattga
     7321 agatgccgca tcacagtcgg ctgcctatgt tgtcatgcca atgagactgt aatgtccctc
     7381 acccgcttgt tgatccgcga tttccgcaac attgaaaccg cggatctcgc tttatctccc
     7441 ggctttaact ttctggtagg tgccaacggc agtggcaaaa ccagcgtgct ggaagccatc
     7501 tatacgctcg gccatggtcg ggcgtttcgc agtttgcaga ttggtcgcgt cattcgccat
     7561 gagcaggagg cgtttgttct ccacgggcga ttacagggcg aagagcgcga gacagcgatt
     7621 ggcttaacca aagacaaaca gggcgacagc aaagtccgca tcgacggtac agacgggcat
     7681 aaggtcgcgg aactggcgca cctgatgcca atgcagttga taacgccaga agggtttact
     7741 ttactcaacg gcggccccaa atacagaaga gcattcctcg actggggatg ctttcacaac
     7801 gaacccggat ttttcaccgc ctggagcaat ctcaagcgat tgctcaagca gcgcaatgcg
     7861 gcgctgcgcc aggtgacacg ttacgaacag ctacgcccgt gggataaaga actgatcccg
     7921 ctggcggagc aaatcagcac ctggcgcgcg gagtatagcg ccggtatcgc ggctgatatg
     7981 gccgatactt gtaagcaatt tctccctgag ttttctctga ctttctcttt ccagcgcggc
     8041 tgggagaaag agacagaata tgctgaggtg ctggaacgta attttgaacg cgatcgccag
     8101 ctaacctaca ccgcgcatgg cccgcataaa gcggacttac gcattcgcgc cgacggtgcg
     8161 ccggtggaag ataccttatc gcgtgggcag cttaagctgt tgatgtgcgc cttacgtctg
     8221 gcgcaaggag agttcctcac ccgtgaaagc gggcggcggt gtctctacct gatagatgat
     8281 tttgcctctg agcttgatga tgagcgtcgc gggctgcttg ccagccgctt aaaagcgacg
     8341 caatcacagg tctttgtcag cgcgatcagt gctgaacacg ttatagacat gtcggacgaa
     8401 aattcgaaga tgtttaccgt ggaaaagggt aaaataacgg attaacccaa gtataaatga
     8461 gcgagaaacg ttgatgtcga attcttatga ctcctccagt atcaaagtcc tgaaagggct
     8521 ggatgcggtg cgtaagcgcc cgggtatgta tatcggcgac acggatgacg gcaccggtct
     8581 gcaccacatg gtattcgagg tggtagataa cgctatcgac gaagcgctcg cgggtcactg
     8641 taaagaaatt atcgtcacca ttcacgccga taactctgtc tctgtacagg atgacgggcg
     8701 cggcattccg accggtattc acccggaaga gggcgtatcg gcggcggaag tgatcatgac
     8761 cgttctgcac gcaggcggta aatttgacga taactcctat aaagtgtccg gcggtctgca
     8821 cggcgttggt gtttcggtag taaacgccct gtcgcaaaaa ctggagctgg ttatccagcg
     8881 cgagggtaaa attcaccgtc agatctacga acacggtgta ccgcaggccc cgctggcggt
     8941 taccggcgag actgaaaaaa ccggcaccat ggtgcgtttc tggcctagcc tcgaaacttt
     9001 caccaatgtg accgagttcg aatatgaaat tctggcgaaa cgtctgcgtg agttgtcgtt
     9061 cctcaactcc ggcgtttcca ttcgtctgcg cgacaagcgc gacggcaaag aagaccactt
     9121 ccactatgaa ggcggcatca aggcgttcgt tgaatatctg aacaagaaca aaacgccgat
     9181 ccacccgaat atcttctact tctccactga aaaagacggt attggcgtcg aagtggcgtt
     9241 gcagtggaac gatggcttcc aggaaaacat ctactgcttt accaacaaca ttccgcagcg
     9301 tgacggcggt actcacctgg caggcttccg tgcggcgatg acccgtaccc tgaacgccta
     9361 catggacaaa gaaggctaca gcaaaaaagc caaagttagc gccaccggtg acgatgcgcg
     9421 tgaaggcctg attgcggtcg tttccgtgaa agtgccggac ccgaaattct cctcccagac
     9481 caaagacaaa ctggtttctt ctgaggtgaa atcagcggtt gaacagcaga tgaacgaact
     9541 gctggcagaa tacctgctgg aaaacccaac cgacgcgaaa atcgtggttg gcaaaattat
     9601 cgatgctgcc cgtgcccgtg aagcggcgcg tcgcgcgcgt gaaatgaccc gccgtaaagg
     9661 tgcgctcgac ttagcgggcc tgccgggcaa actggcagac tgccaggaac gcgatccggc
     9721 gctttccgaa ctgtacctgg tggaagggga ctccgcgggc ggctctgcga agcaggggcg
     9781 taaccgcaag aaccaggcga ttctgccgct gaagggtaaa atcctcaacg tcgagaaagc
     9841 gcgcttcgat aagatgctct cttctcagga agtggcgacg cttatcaccg cgcttggctg
     9901 tggtatcggt cgtgacgagt acaacccgga caaactgcgt tatcacagca tcatcatcat
     9961 gaccgatgcg gacgtcgacg gctcgcacat tcgtacgctg ctgttgacct tcttctatcg
    10021 tcagatgccg gaaatcgttg aacgcggtca cgtctacatc gctcagccgc cgctgtacaa
    10081 agtgaagaaa ggcaagcagg aacagtacat taaagacgac gaagcgatgg atcagtacca
    10141 gatctctatc gcgctggatg gcgcaacgct gcacaccaac gccagcgcac cggcattggc
    10201 tggcgaagcg ttagagaaat tggtgtctga gtacaacgcg acgcagaaaa tgatcaaccg
    10261 catggagcgt cgttatccga aagcaatgct gaaagagctt atctatcagc cgacgctgac
    10321 ggaagccgac ctctctgatg agcagaccgt tacccgctgg gtgaacgcgc tggtcagcga
    10381 actgaacgac aaagaacagc acggcagcca gtggaagttt gatgtccaca ccaatgccga
    10441 acaaaacctg ttcgagccga ttgttcgcgt gcgtacccac ggtgtggata ctgactatcc
    10501 gctggatcac gagtttatca ccggtggcga atatcgtcgt atctgcacgc tgggtgagaa
    10561 actgcgtggc ttgctggaag aagatgcgtt tatcgaacgt ggcgagcgtc gtcagccggt
    10621 agccagcttc gagcaggcgc tggactggct ggtgaaagag tcccgtcgcg gcctctccat
    10681 ccagcgttat aaaggtctgg gcgagatgaa cccggaacag ctgtgggaaa ccaccatgga
    10741 cccggaaagc cgtcgtatgc tgcgcgttac cgttaaggat gcgattgccg ctgaccagtt
    10801 gttcaccacg ctgatgggcg acgccgttga accgcgccgt gcgtttattg aagagaacgc
    10861 cctgaaagcg gcgaatatcg atatttaatg gcgctaacca tgcgagcgtg cctgatgcgc
    10921 tacgcttatc aggcctacga aaatcttgca atgtattgaa ttttcatgat tttgtaggcc
    10981 ggataaggcg ttcacgccgc atccggcaag aagctataag aaaagggcgg agatcatctc
    11041 cgcccttttt atttctgcaa tccgcaatta tcatcccgca aatctatact ttttgggtca
    11101 acacgtaacg ctttacaggt gattatcatg gggctttttg atgaagttgt cggtgccttt
    11161 ctgaaaggcg atgcggggaa atatcaggct attttaagtt gggttgagga gcagggcggc
    11221 attcaggtgc tgctggaaaa actgcaaagt ggcggcttag gggccattct ctcaacctgg
    11281 ctgagtaatc aacagggcaa tcaatcggtt agtggcgagc aactggaatc ggcgctcggc
    11341 acaaatgcgg tgtccgatct tggacaaaaa cttggcgtgg ataccagtac agcttccagt
    11401 ttactggcag aacaattgcc gaagattatt gatgcgctct caccgcaagg tgaagtgtct
    11461 ccacaagcca ataacgatct gctttccgca ggcatggaac tgctgaaagg gaaactcttc
    11521 cgctaagcaa atggggagca tgcaacccgc gttgcatccc ccttttccct cgggtgttgt
    11581 actgattttt gagcggaatc gcgttagcat gggtcaggaa ctaatctacc tggggaactc
    11641 atggctatca aactcattgc tatcgatatg gatggcaccc ttctgctgcc cgatcacacc
    11701 atttcacccg ccgttaaaaa tgcgattgcc gcagctcgcg cccgtggcgt gaatgtcgtg
    11761 ctaacgacgg gtcgcccgta tgcaggtgtg cacaactacc tgaaagagct gcatatggaa
    11821 cagccgggcg actactgcat tacttataac ggcgcgctgg tacagaaggc cgctgatggt
    11881 agcaccgtgg cgcaaactgc tctcagctat gacgactatc gtttcctgga aaaactctct
    11941 cgcgaagtcg gttctcattt ccacgccctg gaccgcacca cgctgtacac cgccaaccgt
    12001 gatatcagct actacacggt gcatgaatcc ttcgttgcca ccattccgct ggtgttctgc
    12061 gaagcggaga aaatggaccc caatacccag ttcctgaaag tgatgatgat tgatgaaccc
    12121 gccatcctcg accaggctat cgcgcgtatt ccgcaggaag tgaaagagaa atataccgtg
    12181 ctgaaaagtg cgccgtactt cctcgaaatc ctcgataaac gcgttaacaa aggtacgggg
    12241 gtgaaatcac tggccgacgt gttaggtatt aaaccggaag aaatcatggc gattggcgat
    12301 caggaaaacg atatcgcaat gattgaatat gcaggcgtcg gtgtggcgat ggataacgct
    12361 attccttcgg tgaaagaagt ggcgaacttt gtcaccaaat ccaaccttga agatggcgtg
    12421 gcgtttgcta ttgagaagta tgtgctgaat taatcagtgg acgggcaaac agcataaacg
    12481 cgtttgcccg cttactgatt attggtttac tgcatataca accagaaatt gcagcggtgc
    12541 agtaatgatg tccagcgtaa cagtcactgg cagtaatgct gcggaaacac catccggaat
    12601 gcctctgggt gaatagtatt cataaaatcg cactttaaac ggatgataga acgccatcac
    12661 ctttgagtaa tccatgtttt tatttttctc atggattgtg ccttttaggt tttccagtga
    12721 cctggtacag tgttgaacac cacaggcaaa accataatgc gttgccagtg ccttttcttc
    12781 ttcgttattc cagtagtagt aaagcgatat tgtgccggta aatttctttt tccccgcatt
    12841 taaaacaaat cttgcgtcat cggcaacctg aatattgtgc cggttaagtg ctggatcatt
    12901 gagcatctta acgatgtcat cggcaccatc agtgataagg taatccagcg attcccctac
    12961 aaaaacatag ccttttgttc cattactatc ttttgcctgc gatagcccag tgatggtgtc
    13021 tgactgcgat tgatactttc ctgaactacc ctttgcggta tgggaaacca gagcatacga
    13081 gatacagcct gaaagcatca gcgccagtgg gaataaaccg gcagccttaa aaaatccctt
    13141 aaaattcaac ttcatgatta atcctttaaa cgagtagcta agccgttgaa agcatactct
    13201 tctttccctc ctgtttagcg cctgatatcc cttttcagat ttctgcccga cgcatgtcat
    13261 ttttttatgc attgttcttt ttgtgatcta aattgtagta caacaatata agtttgtact
    13321 acattacgca cacggcaaac gcgaacgtca tcacgctggt actacaaagt tgccgcgtta
    13381 tgcatcgatc ggggtaaagt agagaagaac atacagagca caaggactct ccatgactct
    13441 caataaaacc gatcgcattg tcattacgct gggtaaacag atcgttcacg gcaaatacgt
    13501 acctggctcg ccacttccgg ctgaggcgga gctctgtgaa gagtttgcaa cctcgcgcaa
    13561 catcatccgt gaggtgttcc gttcgctgat ggcgaagcgg ctgattgaaa tgaaacgtta
    13621 tcgcggggcg tttgtggcac cgcgtaacca gtggaattac ctcgacactg acgtactgca
    13681 atgggtgctg gaaaacgact acgacccacg gcttatcagt gccatgagcg aagtgcgaaa
    13741 tctggtggaa ccggcgattg cccgttgggc agcagagcgc gcgacttcca gcgatctggc
    13801 gcagattgaa tcggcgctga acgagatgat tgccaacaat caggaccgcg aagcgtttaa
    13861 cgaagcggat attcgctacc acgaggcggt gctgcagtcg gtacataacc cggtgttaca
    13921 gcaacttagc attgcgatca gttcgttgca gcgggcggtt tttgaacgaa cctggatggg
    13981 cgatgaggcc aacatgccgc aaacgctcca ggaacataag gcgctgttcg atgcaatacg
    14041 gcatcaggac ggcgatgcgg cagagcaggc ggcgcttacc atgatcgcca gctcgacacg
    14101 aaggttaaag gaaatcacat gacagctcgc tacatcgcaa ttgactgggg atcgaccaat
    14161 ctgcgcgcct ggctttatca gggcgaccac tgcctggaga gcaggcaatc agaagcaggc
    14221 gtcacgcgcc tgaacggaaa atctccggct gcggtgttag cataagtcac gaccgactgg
    14281 cgtgaagaaa atacgccagt ggtgatggca ggaatggtcg gcagcaatgt cggctggaaa
    14341 gttgctccgt atttatctgt tcctgcccgt ttttcgtcta ttggcgaaca attaacgtct
    14401 gttggcgaca atatctggat tattcccgga ttatgcgtct ctcatgacga taaccacaat
    14461 gtgatgcgcg gcgaagaaac acaattgatc ggcgcgcgag ctctggctcc ttcctctctt
    14521 tatgttatgc ccggtacgca ttgcaaatgg gtgcaggccg atagccagca aatcaacgat
    14581 tttcgcaccg tgatgaccgg tgaattacat catttactgt taaatcactc attgattggc
    14641 gcaggtttgc cgccgcagga aaactctgcc gatgccttcg cggctggcct tgagcgcggc
    14701 cttaatgcgc ccgccatatt gccgcagctt tttgaagttc gcgcctcgca tgtgctggga
    14761 acacttcccc gcgaacaggt cagcgaattt ctctctggtt tgttgattgg cgcagaggtt
    14821 gccagtatgc gcgactatgt gacccatcaa cacgccatca cccttgtcgc cggaacatcg
    14881 ctgaccgcgc gctaccagca agcctttcag gcgatgggtt gcgacgtgac ggcggtggcg
    14941 ggcgacacgg catttcaggc tggtataagg agcatcgctc atgcagtggc aaactaaact
    15001 tccgctgatc gccattttgc gcggcattaa gcccgacgag gcgctggcgc atgttggcgc
    15061 ggtgattgac gccgggttcg acgcggttga aatcccgctg aattccccac aatgggagca
    15121 aagcattccc gccatcgttg atgcgtatgg cgacaaggcg ttgattggcg caggtacggt
    15181 actgaaacct gaacaggtcg atgcgctcgc caggatgggc tgtcagctca tcgttacgcc
    15241 caatatccat agtgaagtaa tccgccgtgc ggtgggctac ggcatgaccg tctgccccgg
    15301 ctgcgcgacg gcgaccgaag cctttaccgc gctcgaagcg ggcgcgcagg cgctgaaaat
    15361 atttccgtca tcggcttttg gtccgcaata catcaaagcg ttaaaagcgg tattgccatc
    15421 ggacatcgca gtctttgccg ttggcggcgt gacgccagaa aacctggcgc agtggataga
    15481 cgcaggttgt gctggggcgg gcttaggcag cgatctctat cgcgccgggc agtccgtaga
    15541 acgcaccgcg cagcaggcag cagcatttgt taaggcgtat cgagaggcag tgcaatgaaa
    15601 atcaccaaaa ttactacgta tcgtttacct ccccgctgga tgttcctgaa aattgaaacc
    15661 gatgaaggcg tggtcggttg gggcgagccc gtgattgaag gccgcgcccg tacggtggaa
    15721 gcggcagttc acgagctggg tgactatttg attggtcagg atccttcgcg catcaatgac
    15781 ttatggcaag tgatgtatcg cgccggattt tatcgtggcg gtccaatcct gatgagcgcc
    15841 attgccggga tcgaccaggc gttatgggat atcaaaggca aagtgctgaa tgcgccggtc
    15901 tggcaactga tgggcggcct ggttcgcgac aaaattaaag cctacagttg ggtcggcggc
    15961 gatcgtccgg cggatgttat cgacggcatt aaaaccctgc gcgaaatcgg cttcgatacc
    16021 ttcaaactga acggttgtga agaactgggg ctaattgata actcccgcgc ggtagatgcg
    16081 gcagtcaaca ccgtggcaca aattcgtgaa gcttttggca atcagattga gtttggtctt
    16141 gatttccacg gtcgcgtcag cgcgccaatg gcgaaagtgc tgattaaaga actggagccg
    16201 tatcgcccgc tgtttattga agagccggtg ctggcggaac aagccgaata ctacccgaaa
    16261 ctggcggcac aaacgcatat tccactggcg gcgggtgagc gcatgttctc acgtttcgat
    16321 tttaaacgcg tgctggaggc aggcggtatt tcgattctgc aaccggatct ctcccatgca
    16381 ggcggtatta ccgaatgcta caaaattgct ggaatggcag aagcctatga tgtgaccctt
    16441 gcgccgcact gtccgctcgg accgattgca ctggcggctt gcctgcatat cgactttgtt
    16501 tcctataacg cggtacttca ggaacaaagt atgggcattc attacaacaa aggcgcggag
    16561 ttactcgact ttgtgaaaaa caaagaagac ttcagtatgg ttggcggctt ctttaaaccg
    16621 ttaacgaaac cgggcttagg tgtggaaatc gacgaagcta aagttattga gttcagtaaa
    16681 aatgccccgg actggcgtaa tccgctctgg cgtcatgaag ataacagcgt agcagagtgg
    16741 taattcctgc cacgtaagcc cctcatcggg cactaaaaca gcaatacaaa aatataaccc
    16801 tctgtaaatt acagggcatg gtgagcggct tcgctatgcc caaaatctgg agaaagctcg
    16861 ctatggatat tcccgttaat gcagcaaagc cggggcgtcg gcgttatctg acgctggtga
    16921 tgatctttat tacggtggtc atttgttatg ttgaccgcgc taacctggcc gtggcttccg
    16981 cccatattca ggaagagttc ggcattacga aagcggaaat gggctatgta ttttcggcct
    17041 tcgcctggct ttatacgcta tgccagatcc ccggcggttg gtttttagat cgcgtaggtt
    17101 ctcgcgtgac ttattttatt gcgatatttg gttggtcagt ggcgacttta ttccagggct
    17161 ttgccacggg attaatgtca ttaattggtc tgcgtgcgat aaccggtatt ttcgaagcgc
    17221 ccgctttccc gaccaataac cggatggtga ccagctggtt cccggaacat gaacgcgctt
    17281 ccgccgttgg tttttatacg tctggtcagt ttgtcggtct ggcgtttttg actccgctgc
    17341 tgatctggat tcaggagatg ttgagctggc actgggtgtt cattgtcact ggtggtatcg
    17401 gcattatctg gtcgctgatt tggtttaagg tttatcagcc gccgcgcctg accaaaggta
    17461 tcagcaaagc tgaactggat tacattcgtg atggcggcgg tctggtggat ggtgatgcgc
    17521 cggtgaagaa agaggcgcgt cagccgttaa cagccaaaga ctggaaactg gtgttccatc
    17581 gtaaactgat cggcgtttat cttgggcaat ttgcggtggc ttctacactg tggtttttct
    17641 taacctggtt cccgaactat ttaacccagg aaaaaggaat cacggcgctg aaagcaggct
    17701 ttatgaccac ggtgccattc ctcgcggcgt ttgtcggcgt cctgctctct ggctgggtcg
    17761 cggatctgct ggtacgtaag ggcttttcac tgggctttgc gcgtaaaacg ccgattatct
    17821 gcggcttgct gatctccacc tgcattatgg gcgctaacta caccaacgat ccgatgatga
    17881 ttatgtgcct gatggcgctg gcattcttcg gcaacggttt tgcttcgatt acctggtcgc
    17941 tggtctcttc tctggcaccg atgcgcctga ttggtttaac cggcggcgtg tttaacttcg
    18001 ccggtggtct gggcggcatc accgttccgc tggtggtggg gtacctggcg cagggttacg
    18061 gtttcgcacc tgcactggtt tatatctccg ccgtcgcgtt gattggcgcg ctctcttaca
    18121 tcctgctggt gggcgatgtg aagcgcgttg gctaatcttt caactgtgga atatgcgcca
    18181 taccactgcg cataatccac tttcttaaag ccggtgcggt caggcatcgg ctttttacta
    18241 tcttgccgaa gagttttaaa cgcagtttgc gggtggcgcg ccagtaagcg gtattgagct
    18301 tctctggctg cttcagtaac accgaacgca gaatgtctgc gctatccagc gcatagctaa
    18361 tcccctccag cgaactggcg ctgataaatc ctgccgcttc gccaatcaga aaggcgttgt
    18421 ccttaccgca gacaaaatcc tgccagcgtg agggaaacag caccgtgcat ttttcgcttt
    18481 tcaccgcctt accaaactgg aactgaaagg cgctcatttt ctctttcagc gtcgtgaaac
    18541 gcgtctgacc gtctttcatt ggataagcac cgccaaagat aaaataaccg tctttgctga
    18601 tactccatga ataacagtta gttatcgaat tatcaaagat gcaggagtag aacggcaccg
    18661 gatgtttctc tgcgaaccac tgctggatag cgacatattt acggatttga tgatccgggt
    18721 agagatgtcg ccgcaccatc gagtttgcgc catcggcacc gaccagatag cgggcagtaa
    18781 tatgttgctc ccagccgtct gcacgaaaaa tgacatgcca tttatcatcc tcacgccaga
    18841 tttttcggca caggctatcg tgataaacct caacgctggc ggggatcagc gatttcatcc
    18901 acaagtcgaa agcatggcga ttaatattga tatagcttcg ctggtagtta cgcgtgagcg
    18961 atgcggcgac gtcgacagtt ttgacgctga aaatctgcgg gttggcgatc acatcgacag
    19021 gaagcgtcag tccatcgcga ataaaagatc gctgcgcgtc cggtgccagc agaccgccgc
    19081 aaggtttgct gaaaccttca gtaccacact ggtgcttttt atccagcgcg atcacctgca
    19141 ttttgcctgc taattttcgc gccaacgccg atcctgccgg gccgaggcca ataatcgcca
    19201 cgtcgaaatg ttccatcttt tcactcacat tcatcacgat ggcaaaagac tattgtctga
    19261 aagtgaagtc cttgtgaagt atgatggcgg gaaccgtttt gtatcaggac cgcgccatga
    19321 aacagataac ctttgctccc cgtaatcacc tgctcaccaa taccaatacc tggacgcccg
    19381 acagccagtg gctggtattt gacgtgcgtc cttctggggc gtcgtttacc ggcgagacca
    19441 ttgagcgtgt gaatatccat acaggcgagg tcgaggttat ctatcgcgcg tcacagggcg
    19501 cacacgtcgg cgtggtgacc gttcatccga agtcagagaa atatgtcttt attcacggcc
    19561 ctgaaaatcc tgatgaaaca tggcattacg acttccatca tcgacgcgga gtcattgctg
    19621 aaggcggcaa ggtgagcaat ctcgatgcaa tggatattac cgcgccgtat acgccaggag
    19681 cgctgcgcgg cggcagccat gtgcatgtct ttagcccgaa cggtgaaagg gtgagcttta
    19741 cctataacga ccatgtaatg catgaactcg atccggcgct ggatttgcga aacgtcggtg
    19801 ttgctgcgcc gtttggcccg gtcaacgtac aaaagcagca tccgcgtgaa tacagcggta
    19861 gccactggtg cgtgctggtg agtaaaacca cgcccacgcc gcagcctggc agcgatgaaa
    19921 tcaatcgtgc ttatgaagaa ggatgggtag gaaatcacgc gctggcgttt attggcgaca
    19981 cactttcgcc aaagggcgag aaagtgccgg agctgtttat cgttgagtta ccgcaagatg
    20041 aagctggctg gaaagcggca ggtgatgcgc cgttaagtgg aacggaaacc accctgcccg
    20101 cgccaccgcg cggcgtcgtg cagcgacgtt taacctttac ccaccatcgg gcttatccgg
    20161 ggttagtcaa cgtcccgcgc cactgggtgc gctgtaatcc gcagggtacg caaatcgcgt
    20221 ttttaatgcg tgatgataac ggcattgtgc aactgtggct tatctcgcca cagggcggcg
    20281 agccgcgcca gttaacccat aacaaaacgg atattcagtc tgcatttaac tggcatccgt
    20341 caggagaatg gttgggcttt gtgctggata atcgaattgc ttgtgcccat gcgcaaagcg
    20401 gcgaggttga gtatttaacc gaaaaccacg ccaatccacc ttctgcggac gccgtggtct
    20461 tctcgccgga tggtcaatgg ctggcgtgga tggaaggtgg ccagctgtgg atcaccgaaa
    20521 ctgatcgcta aatcagttat caggcattct ggccggtgga ataaccgcat tggtggcttg
    20581 cgcgttggct tcgctttttt caacgcgtga gcgcaccgag ctgtctttgc ggaacacatc
    20641 ccacggcagt aaaagcgtgt ccatcacagc ggtaaacggc atatcgagaa tcgccagcga
    20701 cttagtgccc cagttggttt catcgtcgcc aatcatggtg gcgctggcgc gggtgccggg
    20761 atacgttcct tctttccctc cggtatggga catcacgctg gagcatcctc cgagtaatgt
    20821 cattccgctg catatcatga acgctaacaa cacatttctt atcataataa attcatctgt
    20881 tgatcgtggg tgttggcctg atgagttata gcgatccctt gctgaaaata acatcatcat
    20941 tacgtcgcac tgtggcggct atcgcacttt aacgtttcgt gctgccccct cagtctatgc
    21001 aatagaccat aaactgcaaa aaaaagtccg ctgataaggc ttgaaaagtt catttccaga
    21061 cccattttta catcgtagcc gatgaggacg cgcctgatgg gtgttctggc tacctgacct
    21121 gtccattgtg gaaggtctta cattctcgct gatttcagga gctattgatt atgcgtaact
    21181 ttgatttatc cccgctttac cgttctgcta ttggatttga ccgtttgttt aaccacttag
    21241 aaaacaacca gagccagagt aatggcggct accctccgta taacgttgaa ctggtagacg
    21301 aaaaccatta ccgcattgct atcgctgtgg ctggttttgc tgagagcgaa ctggaaatta
    21361 ccgcccagga taatctgctg gtggtgaaag gtgctcacgc cgacgaacaa aaagagcgca
    21421 cctatctgta ccagggcatc gctgaacgca actttgaacg caaattccag ttagctgaga
    21481 acattcatgt tcgtggtgct aacctggtaa atggtttgct gtatatcgat ctcgaacgcg
    21541 tgattccgga agcgaaaaaa ccgcgccgta tcgaaatcaa ctaattccct aaggccgcct
    21601 ggcgcggcct gacatctcca tgctcgccgt cagggagcat atgcgaatct tcggatttgc
    21661 aggtacttac tcgcttctta gaaggagaaa tgactatgcg taacttcgat ttatccccac
    21721 tgatgcgtca atggatcggt tttgacaaac tggccaacgc actgcaaaac gccggtgaaa
    21781 gccagagctt tccgccgtac aacattgaga aaagcgacga taaccactac cgcattaccc
    21841 ttgcgctggc aggtttccgt caggaagatt tagagattca actggaaggt acgcgcctga
    21901 gcgtaaaagg cacgccggag cagccaaaag aagagaaaaa atggctgcat caagggctta
    21961 tgaatcagcc atttagcctg agctttacgc tggctgaaaa tatggaagtc tctggcgcaa
    22021 ccttcgtaaa cggtttactg catattgatt taattcgtaa tgagcctgaa cccatcgcag
    22081 cgcagcgtat cgctatcagc gaacgtcccg cgttaaatag ctaactagct attctctttg
    22141 ccccgccatt ctggtggggc tttttttgtc ttacttactc aacaatgcgt taaatttcga
    22201 ctgtttaaga tatttcggca cgttctttcc cgtgataatg tgcgctataa ggcaaatctg
    22261 cttcaccgat tcagccataa tccgtagcaa ttaaaaatgt tatctcaaga gaaatggacg
    22321 atgagtgata tagcattaac ggtcagtatt ctggctttgg tggcagtcgt cggtttgttt
    22381 atcggcaatg tcaaatttcg cggcatagga ttaggtattg gcggcgtgct gtttggtggg
    22441 atcatcgtcg gccattttgt ttctcaggcg gggatgacat taagtagcga tatgctgcat
    22501 gttattcagg aatttggcct gatcctgttc gtttatacta tcgggattca ggtagggccg
    22561 ggcttctttg cctcattgcg cgtctccgga ttacgcctca acctgtttgc tgttctgatc
    22621 gtcatcatcg gtggtctggt taccgccatc ctgcataaac tgtttgatat tccactgccg
    22681 gtagtgctgg ggattttctc cggtgcggtt accaatacgc cagcgctggg ggcagggcag
    22741 cagattttgc gcgacttggg tacaccaatg gaaatggtcg atcagatggg gatgagttac
    22801 gcgatggcgt atccattcgg catttgcggg attttgttca ccatgtggat gttgcgggtt
    22861 attttccgcg tcaatgtcga gacagaagct cagcagcacg agtcttcacg caccaatggc
    22921 ggcgcgctga tcaagactat caatattcgc gttgagaacc ctaacctgca tgatttagcc
    22981 attaaagatg taccgattct caacggcgac aaaattatct gctcgcgtct gaaacgcgaa
    23041 gaaaccctaa aagttccttc gccagatacc attatccaac tgggcgattt gctgcatctg
    23101 gtggggcagc cagcggattt acataatgcg caactggtga ttggtcagga ggtcgatact
    23161 tcgctgtcca cgaaaggcac tgatttgcgc gtcgagcgtg tggtggtcac caatgaaaac
    23221 gtgctcggaa aacgtattcg cgacctgcac tttaaagaac gctatgacgt tgttatctcg
    23281 cgcctgaacc gtgccggggt cgaactggtc gccagtggcg atatcagcct gcagttcggc
    23341 gatatcctca atctggtggg gcgtccgtcc gcaattgatg ccgttgccaa tgtgctgggg
    23401 aatgcgcagc aaaaactgca acaggttcag atgctgccag tgtttattgg catcgggctt
    23461 ggcgtattgt taggttctat tcccgtcttt gtgcctgggt tcccggccgc gttgaaactg
    23521 gggctggcgg gcggtccgct gattatggcg ttgatcctcg ggcgtatcgg cagtatcggc
    23581 aagctgtact ggtttatgcc gccaagtgcc aacctcgcgc tgcgggagct ggggatcgtg
    23641 ctgttcctct cggtcgttgg tctgaaatct ggtggagatt ttgtgaatac cctggtcaat
    23701 ggcgaagggc taagctggat tggttatggt gccctgatca ccgccgttcc gctgattact
    23761 gttggcattc tggcgcggat gttagccaaa atgaattacc tgaccatgtg cgggatgctg
    23821 gcaggttcca tgaccgatcc tccggcgctg gcgtttgcta ataatcttca tccaaccagc
    23881 ggtgcggcgg cgctctctta cgccactgtc tatccgttag tgatgttcct gcgcattatc
    23941 accccccaat tactggcggt gctcttctgg agtatcggtt aactcttttc cgggtggagt
    24001 cgccgtaaat ggtactccac ctggtattcg ctggcatttc tgaacatcac tgaataattc
    24061 aaaaactccc cgctctcgct atatgacaac gaggtgatcc gcagcagtgg cgtgtgttcc
    24121 gctaccttca tttgccgcgc cagcagtcta tcggcgagca ctggcgtcag gctttcataa
    24181 ttaccgccaa tcaaaatccc gcactcttgt tcaatatatt caaacttcga cccttccaga
    24241 tgttgcagcg aaagattacg gaacagtttt accggcatat agctgtcttc cagcatcagc
    24301 ggtttccctt ccacaaaacg aacgcgacgg gagaagtaga tctgctcgtt gatttgaatc
    24361 cgtaactggc tggcaatggc cggaggcgca ggcatgattt caaaaatcag cacctggctg
    24421 gtgaccgttt ttccctgccg ttttaacacc tccaccagcc cggtcaggct ggcggtttga
    24481 tgcagtacat ctttgcgcac caggtaagtg ccgctgccgt ggcggcggac caccaatccc
    24541 cacgctacca gcaggtcaat ggctttacgg atggtcatcc gcgataccgc aaattcttcc
    24601 gccaactttt tttcaccggg aagaaggcta tttagcgtga aatctgcgga attaggtcga
    24661 attcttaacc gctccgcaat gcttttgtag atcatctcta gatctctttt ttgcctgcaa
    24721 gcacgggtaa tgcgcccaca agttctgctt aatcgattga aaaaacatct tttttttaaa
    24781 gatgtgttcg atgctgtgta cctgtgctca cagatgtcta ctttttcgcg aaaacgtaga
    24841 tctctaccgc ccaacgaaaa gcatgaaagc gatcacgaat cccattgggt cgtcatgttc
    24901 tgccgatcgc atcttcctat cctcgctcca ggcctgccgc ataaccaatc aggcttccta
    24961 cttacagaat tgagaaaaga ggatgtggaa atgctcagtc aaattcaacg ctttggcggc
    25021 gcgatgttca cgccagtgct gctgtttccc ttcgccggga ttgtggtggg tcttgccatc
    25081 ttgctgcaaa acccgatgtt tgtcggggaa tcactgaccg atccgaacag tttattcgcg
    25141 caaatcgtac acattattga agagggcggt tggacggtgt tccgtaatat gccgctgatt
    25201 tttgctgtcg gtttacccat tggccttgct aagcaagcgc aggggcgtgc ttgtctggcg
    25261 gtgatggtga gtttcctgac ctggaactat ttcatcaacg cgatgggaat gacctgggga
    25321 agctacttcg gcgtcgattt cactcaggac gcggtggcag gtagcggtct gacaatgatg
    25381 gccgggatta aaaccctcga taccagcatt atcggcgcaa ttatcatttc cggcattgtg
    25441 acggcgctgc ataaccgtct gttcgataaa aaactgccgg tttttctcgg cattttccag
    25501 gggacgtctt atgtggtgat tatcgccttc ctggtgatga tcccctgtgc ctggctgacg
    25561 ttgctcggct ggccaaaagt acaaatgggg attgaatctc tgcaagcgtt cctgcgttcg
    25621 gcgggtgcgc ttggggtgtg ggtttacacc ttcctcgaac gtattctgat cccaaccggt
    25681 ttacaccact tcatctacgg accgtttatc tttggtccgg cagctgttga aggcggcatt
    25741 cagatgtact gggcgcagca tctgcaagag ttcagtctga gcgccgagcc gctgaaatcg
    25801 ttgttcccgg aaggcggttt tgccctgcac ggtaactcaa aaatctttgg tgccgtgggc
    25861 atttctttag cgatgtactt cactgccgca ccggaaaatc gggtaaaagt ggcgggcttg
    25921 ctgattcccg caaccttaac cgccatgctg gtgggaatta ccgaaccgct ggaatttacc
    25981 ttcctgttca tttcaccgtt gctgtttgcg gtacacgctg tgcttgcggc ctcaatgtcg
    26041 accgtgatgt atctctttgg tgtggtgggc aacatgggcg gaggtctgat tgaccaggtt
    26101 ttaccgcaaa actggatccc gatgttcagc aaccacgcgg atatgatgct gacccaaatc
    26161 gccattgggt tgtgctttac cctgctgtac ttcgtggttt tccgcacact gattctgcag
    26221 ttcaacatgt gcacgccggg acgtgaagat gcggaagtga aactctactc aaaagccgaa
    26281 tacaaagcct cgcgaggcca aaccaccgcg gcagagccaa aaaaagagct ggatcaggct
    26341 gccggtatcc tgcaagccct gggcggggtc ggcaatatct ccagcattaa caattgtgcg
    26401 acgcgtctac gcattgcact gcatgacatg tcacaaacgc tggatgacga agtctttaaa
    26461 aagctgggag cgcacggcgt cttccgtagt ggcgatgcca ttcaggtgat cattggtctg
    26521 catgtatccc agctgcgtga acagctcgat agcttaatta attctcatca atcagcagaa
    26581 aatgttgcca ttacggaggc agtataatga ccaaattctc agtggttgtc gcaggcggtg
    26641 gaagcacctt tacgccaggc atcgtgttga tgctcctggc gaatcaggag cgtttcccgc
    26701 ttcgtgcact gaaattttat gataacgatg gcgcgcggca ggaagtgatt gccgaagcct
    26761 gtaaagtcat ccttaaagag aaagcgccgg acattgcgtt tagttacacc accgatcctg
    26821 aagtggcgtt cagcgacgtt gattttgtca tggcgcacat ccgcgtcggc aaatacccga
    26881 tgcgcgaact ggatgaaaaa atcccactgc gccacggtgt tgttggtcag gaaacttgcg
    26941 gacccggcgg aatagcgtac ggcatgcgtt ccattggcgg cgtcctggaa ctggtggatt
    27001 atatggaaaa atattcacca aacgcctgga tgctcaacta ctccaacccg gcagcaattg
    27061 tcgcagaagc cacgcgtcgt ctgcgcccga atgcgaaaat cctcaacatc tgtgacatgc
    27121 caatcggtat tgaaagccgg atggcgcaaa ttgttgggct gcaagatcgc aaacagatgc
    27181 gcgtgcgcta ctacggcctg aaccactggt ggtcggcgat atcccgcagt ttcagaaagg
    27241 gttaatgagt cagcaagtgg cggtggaaaa actggtcgtg gacgcctggg aacagcgttc
    27301 atatcagcac ctgtggtagg cgattacgtt gtcgaaaacg gtaccgagcg cctcggtcgc
    27361 caaagctatt ctggatgagt tgctggaggc caacaaagcg tactggccag agttacgtta
    27421 attgtgtgga ctggcatccg ttgggtgctg gtccgtattc tttagcacgg ctgacggcgc
    27481 ataaccaaat ttatgtttaa aggctttgct gaaatgaaag gagtcgaaga agttgagcgt
    27541 atcagcgact tcgccaaccg agtttccttg ttgctttaat agcgatagcg ccagatccag
    27601 acgcgcatcg aggtaatatt ctttcggcgt cttgccggta tagcgtaaaa acagacgacg
    27661 caaccaggct tcgctacagg ggatcgtggc agccatatca gctacgctcc agcgttgttg
    27721 cagactggca tgtaaagtgg caattaattt ttcaatttgc cgccgttgtg gatctttttt
    27781 accatctgcg tacataagac aaatccactg ataaataatt ttcgtcagaa aagcgactgc
    27841 cagattattt tttattgctt ctggtgaagt tattaactca gcaacttcgg tgagttcctg
    27901 attataaatt tcgccattat aaataacgct ttgctgacca acgggaatat ccatcatact
    27961 ggtgggggta aattccatcc agtactgttc ccagactaaa ccttcacagt gataagagtg
    28021 aatatccatt ggctttaaga atataataca gttgccatgc agtgttattt gttcgccatt
    28081 ctttaaaaga attttcccac atccttgtac ggtatacacc gcaacccatg agttggcgat
    28141 aagcggtttc ttcttgtccc gcggccagat cacccggtaa tgctcgtcgg cgtaggtatg
    28201 ccagagcgag atcagagaaa ttccgctgga gatcacattt tcgttaatca ataagggaat
    28261 attgtacgga gtttcgtttt ttacatctga actttgcaat tttccattca ttttaatatc
    28321 cctccggcga attatgctca caacctgaac ttaacagaaa acaatccgtt cagggatacg
    28381 acgtttgtat tttaagaatc tgactgcctg acccgacgca ttttacctct ccctatattc
    28441 atgcgtccgg gacataaaca taaataaggg ctatgagatg aattcgttac aaatcttgag
    28501 ttttgtcggt tttacgctgc tggtggcggt gatcacctgg tggaaggtcc gcaaaacaga
    28561 taccggatcg caacaaggct attttcttgc cggacgttca ctaaaagcgc cggttattgc
    28621 cgcttcgtta atgctaacca acctttccac ggaacaactg gtcggccttt ccgggcaggc
    28681 ctacaaaagc ggcatgtcgg tgatgggctg ggaagtgact tcagcggtga cgctgatctt
    28741 cctcgcgcta atctttttac cgcgctatct gaagcgcggc attgccacca tccccgattt
    28801 tctggaggaa cgttatgata aaacgacgcg tattatcatc gacttctgct tcctcattgc
    28861 caccggcgtc tgctttctgc cgattgttct ctactccggc gcgttggcgc tcaacagcct
    28921 gtttcacgtc ggggaatcgc tacagatttc tcacggtgcg gctatctggc tactggtaat
    28981 tttgcttggt ctggcgggaa ttttgtatgc ggtgatcggc ggactgcgcg caatggcagt
    29041 ggcggactcc atcaacggta ttgggctggt tatcggcggg ttgatggtgc cggtatttgg
    29101 cctgatcgcg atgggcaagg gcagctttat gcagggcatt gagcaactca ccaccgttca
    29161 cgccgagaaa ttaaactcaa tcggtggccc gaccgatccc ttgccgattg gcgcggcatt
    29221 taccggtttg attctggtga acacctttta ctggtgtaca aatcagggca tcgtgcaacg
    29281 cacgctggcg tcaaaaagcc tggcggaagg gcaaaagggg gcgctgttaa cggcggtgct
    29341 gaaaatgctc gacccgctgg tactggtgct gccagggttg attgcgtttc atctgtatca
    29401 ggatttaccg aaagccgaca tggcctaccc gacgctggtc aataacgttc tgccagtgcc
    29461 aatggtgggt ttcttcggcg cggtgttatt tggtgcggtg atcagtacct tcaacggctt
    29521 tctgaatagc gccagtacgt tattcagtat aggtatttac cgtcgcatca ttaaccagaa
    29581 tgccgagccg cagcagctgg tcaccgtcgg gcgcaaattt ggtttcttta tcgctatcgt
    29641 ttcggtgctg gtcgcgccgt ggatcgccaa cgcgccgcag gggctgtata gctggatgaa
    29701 acagctcaac ggcatttaca acgtgccgct ggttaccatc atcattatgg gctttttctt
    29761 cccgcgcatc ccggcgctgg cggcaaaagt ggcgatgggg attggcataa tcagctacat
    29821 caccatcaac tatctggtga agttcgactt ccatttcctc tatgtgctgg cctgtacgtt
    29881 ctgcatcaac gtggtcgtga tgctggtgat cggttttatc aaaccgcgcg ccacgccgtt
    29941 caccttcaaa gatgcgtttg cggtagacat gaaaccgtgg agaaacgtca agatcgcgtc
    30001 aattggcatc ctgttcgcga tgattggcgt ctatgccggg ctggctgaat tcggcggcta
    30061 cggtacgcgc tggttagcga tgatcagtta tttcattgcc gccgtagtga ttgtctacct
    30121 gatttttgac agctggcggc atcgtcacga cccagccgta acctttactc ccgacgcgaa
    30181 ggatagccta tgaaacgccc caattttctg ttcatcatga ccgataccca ggccaccaat
    30241 atggtcggtt gctatagcgg taaaccgctg aatacgcaaa atattgatag tctggcggcg
    30301 gaaggtattc gctttaattc cgcctacacc tgttcaccgg tttgtacgcc tgcacgcgcc
    30361 ggactattta ccggtatcta cgctaaccag tccggcccgt ggaccaacaa cgtcgcgcca
    30421 ggcaaaaaca tctccactat ggggcgctac tttaaggatg ccggctatca cacctgttac
    30481 atcggcaaat ggcatctcga cggtcatgac tatttcggca ctggcgagtg tccgccggag
    30541 tgggacgctg attactggtt cgatggggcg aactacctta gcgaactgac ggaaaaagag
    30601 atcagcctgt ggcgcaatgg cctaaacagc gttgaggatt tacaggcgaa ccatatcgac
    30661 gaaaccttca cctgggcgca ccgcatcagc aatcgggcgg tagattttct gcaacagccc
    30721 gcgcgcgccg aggaaccctt cctgatggtg gtttcgtatg atgagccgca tcacccgttc
    30781 acctgtccgg tggagtattt agagaaatac gctgattttt actacgatct gggcgagaaa
    30841 gctcaggatg acctggcgaa caaaccggaa catcaccgct tatgggcgca ggcgatgcca
    30901 tcgccagtcg gtgatgacgg gctttatcac catccgctct attttgcctg taatgacttt
    30961 gttgatgacc aaatcggacg ggtcatcaat gccttaacgc cagagcaacg tgaaaatacg
    31021 tgggtcattt atacttccga tcacggcgaa atgatgggcg cacataagct gatcagtaaa
    31081 ggagcggcga tgtatgacga catcacccgt attccgctga tcatccgttc gccgcaaggg
    31141 gagcggcgac aggtcgatac gccagtcagt catatcgatt tactgccgac aatgatggcg
    31201 ctggcagata ttgaaaaacc agagattctg ccgggggaaa atatccttgc cgtgaaagag
    31261 ccacgcggtg taatggtgga atttaaccgc tacgagattg agcatgacag ctttggcggt
    31321 tttattccgg tgcgttgctg ggtgacggat gactttaaac tggtactcaa cctcttcacc
    31381 agtgatgaac tttacgatcg ccgtaatgac ccaaatgaaa tgcataacct gatcgatgat
    31441 atccgttttg cagacgttcg cagcaaaatg catgacgcct tattggatta catggacaaa
    31501 attcgcgatc cgttccgcag ttaccaatgg agcctgcgtc cgtggcgtaa agatgcactg
    31561 ccgcgctgga tgggggcatt tcgtccacgc ccacaagatg gctattcgcc ggtggtacgt
    31621 gactatgaca ccggcctacc gacgcaagga gtgaaagtgg aagagaaaaa acagaagttc
    31681 tgacgataat gcccgctgat gaacacccgg cgggcattat tcaggctgct taaaccagtg
    31741 aaccgccggt caggaaggaa aaaatcagga atatcagcaa aatgctgcac acgatgccga
    31801 taccaaaaag caaggtatgg aaagccagcg tcccgccgcc aaaaaccagc gcgccgcgaa
    31861 tgccccagcg attttcctta cgcataccgc caataaatgc gcaaagtagc gcagcaatgg
    31921 ccagtgcgat accgctttga tcgaggattt tatcgacatc tacgtcctct tttttctcca
    31981 cggtggtaat tttttcgcct tttattccgg cgagaagacc tcttttaatt tcagccgttt
    32041 tgtccgccac gatgctttca aacgttggcg gtggtgcgga aaaagggccg aatgaaaagt
    32101 ggataatccc catcattaaa gcgatggcac cgaacagcat gcccaatgaa ctgattttat
    32161 tttgagctat gatacccatc tgacctcctt gtagcggatg gcgaaagcat atcaggaaac
    32221 gggcggacga aattcttaaa aaaaaaccgc taaaggtaga gcgttagtga aattgcaaag
    32281 gagcaacaat gaaaatttcc cgccttggag aagcgcccga ttaccgcttc tcgctggcta
    32341 atgagcgtac ttttctggcg tggatccgta ccgcactagg atttctggcg gcgggggttg
    32401 ggcttgatca acttgcgcca gatttcgcca cgcccgtcat tcgtgaactg ctggcgttgt
    32461 tgttgtgtct gttttccggc gggttagcga tgtatggcta cctgcgctgg ttacgcaatg
    32521 aaaaagcgat gcgtctgaaa gaggacctgc cctacaccaa cagcttatta atcattagct
    32581 taatattgat ggtcgtggcg gtgatcgtta tgggactggt gttgtatgcc ggatagccgc
    32641 aaagccagac gcattgccga cccaggactg cagccggagc gcacatcact ggcgtggttt
    32701 cgtaccatgc tgggctacgg cgcgttgatg gcgttggcta tcaaacacaa ctggcaccag
    32761 gcgggcatgt tattctggat ttcgattggc attctcgcca tcgtggcgct gatcctctgg
    32821 cactacaccc gtaatcgcaa tttaatggat gtcacgaata gcgatttttc ccaatttcac
    32881 gtagtccgtg acaaattttt gatctccctc gcggtgttat ctctcgcaat actgtttgct
    32941 gtaacgcata tacatcaact tatcgtattt attgagagag tcgcatgaca ggaagtcagg
    33001 tcattgatgc tgaagaggac aggcataaac tggttgtaga gtacaaggat gctctgcaac
    33061 ctgccgattt ttatcacaat ttcaaacagc ggggcatccg ctccgtacag ctcattccct
    33121 atcttgaatt tgatgaccgg ggcgatctga cggccgcttc ggtgacagca gagctgtggg
    33181 ggaaattttt aattgccctg ttcgagtgtt gggtgcgggc agatatcagt cgtatctcga
    33241 ttgagctttt tgacgccacc ttacaaaaat ggtgtggcag cgaaaacccg caaccgcgcc
    33301 gtgactgcca ggcgtgcgac tggcatcgcc tgtgcccgca cgctcgccag gagacgccag
    33361 acagcgtgct ttgtgcgggc tatcaggcgt tttattctta ctccgcaccg catatgcggg
    33421 tgatgcgtga tctgataaag cagcatcgtt ctcccatgga gctgatgacg atgctgcggt
    33481 gacgtgcgct taaacgggct gcccctgatg cgacatccgc gtcgccagcg gcagccagca
    33541 cagcacgatc aacaatccca ttaaggtcat caacaacccc aggctgccct gaccggtttg
    33601 cggcaacatc gcagagagcg acgccagcac gccggaacca atgttttgca gaccgccgac
    33661 cagcgcgcca gccgtgcccg ccaggaaggg gaacggctcc atcgcgccgc tggtcgccag
    33721 cggaaacagc atcccggcac cgaaaaagaa cagcgcggcg ggaacgagca gcgtccagac
    33781 attcatcacg ccaaaccagt cggggatcca catcagcaag ccagccagca ggcagcagat
    33841 aaccgactgc cacattaacg tggagaagcg tttattggga cgtccggcaa accatgcgcc
    33901 aaaaaacgct gccggaatcg gcagaataaa caaaatactg accgtcatac tgctcagccc
    33961 taacaccgcg cccatcagca cgccggagca ggcttcaaag gcggcaatcc cggccagacc
    34021 gccaatcagc atcagcaaat aacagttaaa accgctgtta ccgaaaagcg ttttataact
    34081 ggtaagcagg cgcgtgcgcg gtgcatcgac cggacgcgtt tccggcatcc agcgggccat
    34141 actgaaggtc acaccagcac aaagaaccaa caaaaagaga taacaggcgc gccagttcca
    34201 catcgtatcc agcagaccgc cgattagcgg tgcgagcaac ggactgacga gaatccccat
    34261 gtttaacagg ctgttagcat ggcgcaactg tgtccgttca tataaatctc gcggcaaagt
    34321 acgcgccatt acgccgccaa cgccggtgcc catcccctgc atcgcgctgg cggcaatcaa
    34381 caccgtcaaa ctggaggtcg tgaccgcgac cagcgttgcc agcataaaaa tggacattcc
    34441 gacgaggatc accggtcggc ggcccacgcg gtcggaaatc gggccataaa acagctgtga
    34501 gacaccgtaa gtcagcagat aagcgcccat tacgctctgc accgcccctt cacggacgtt
    34561 gagatcgcgc gccatatcgg caatagctgg aatataaatg gtttgcgcca tctgaccgac
    34621 ggccacgagt aataccaaca tcaataacaa attgacgttt ctttgccttt tcattatcac
    34681 ggatgctttt ataaaaataa ttagaataag tcactacgca ttcccataaa tgcgggagag
    34741 gaatctatca cattgatgaa tgttagccag atatacgcca ggaatgggga attgtttagc
    34801 ggcagaatat gtaaacaaaa gcggcaataa atgttgccgg gagggattac gtgcgaactg
    34861 taaccctctc cccaaagggg tgagggggaa accaccgttc cctttcagaa aatgagctaa
    34921 acattgcacg ggacggtccc ctcgcccctt tggggagagg gctagggtga ggggcacagg
    34981 cagcgcaggt gcaaacattc tgctggtaga agaagccccc aactccaacc cgcacgctaa
    35041 atataaaagg ggagcggtta cccgctcccc tttggtgcga cttgaatctg aattacttca
    35101 ggtatttcag aacagcatca agcagttgca gtgctgcaac aatgagtttg aggataagaa
    35161 tggcgatatc caccaggctc atacgcgtct cctgtggttc aggagcactg ccagctgacg
    35221 tacctttccg ctgcctgttg ccagctgttg tgcacgcggt atcttaagga gaaacgttgt
    35281 gcttgttgac ataacacagt gtgctcacgc ggggttaagg cgctgacggc accacccgtt
    35341 tcagccagga cttagtgcgc cgggtaacga tgcggccccc gcataacaca ttgcgtacag
    35401 tgatattata gaagtttact gtataaataa acagtaatat ttggacaaaa cgcaaactgt
    35461 gatcgatact caactatgcc ctaaatacgt caaaattcgt gccgaaattg cgcgttctgc
    35521 gcggaacacg tatactttca gtgttgacat aatacagtgt gctttgcggt taccagccgc
    35581 aggcggctga tgaaacctcg ctccggcggg gttttttgtt acctgcaatt cagtacaaaa
    35641 cgtgatcagc ccctcaattt tcccttgctg aaaaattttc cattgtctcc cctgtaaagc
    35701 tgtgcttgta taaatattgt taaacacaaa accaacaagg tccccaatga ctacttccat
    35761 gttcaacgca aaactactac caactgcgct atccgccgca gtggtcgtcg tgcgtgtggt
    35821 ggtggtcgtc ggcaatgcgc cgtagggact ggaacaacac acgattccaa aaccccgccg
    35881 gcgcaaaccg ggcggggttt ttcgtttaag cacctcccgg aaagtcggcc cagaagaaaa
    35941 ggactggagc atggcaagtt cgggcacaac atcgacgcgt aagcgcttta ccggcgcaga
    36001 atttatcgtt catttcctgg aacagcaggg cattaagatt gtgacgggca ttccgggcgg
    36061 ttctatcctg cctgtttacg atgccttaag ccaaagcacg caaatccgcc atattctggc
    36121 ccgtcatgaa cagggcgcgg gatttatcgc tcaggggatg gcgcgcaccg acggtaaacc
    36181 ggcggtctgt atggcctgta gcggaccggg tgcgactaac ctggtgaccg ccattgccga
    36241 tgcgcggctg gactccatcc cgctgatttg catcaccggt caggttcccg cctcgatgat
    36301 cggcaccgac gccttccagg aagtggacac ctacggcatc tctatcccca tcaccaaaca
    36361 caactatctg gtcagacata tcgaagaact cccgcaggtc atgagcgatg ccttccgcat
    36421 tgcgcaatca ggccgcccag gcccggtgtg gatagacatt cctaaggatg tgcaaacggc
    36481 agtttttgag attgaaacac agcccgctat ggcagaaaaa gccgccgccc ccgcctttag
    36541 cgaagaaagc attcgtgacg cagcggcgat gattaacgct gccaaacgcc cggtgcttta
    36601 tctgggcggc ggtgtgatca atgcgcccgc acgggtgcgt gaactggcgg agaaagcgca
    36661 actgcctacc accatgactt taatggcgct gggcatgttg ccaaaagcgc atccgttgtc
    36721 gctgggtatg ctggggatgc acggcgtgcg cagcaccaac tatattttgc aggaggcgga
    36781 tttgttgata gtgctcggtg cgcgttttga tgaccgggcg attggcaaaa ccgagcagtt
    36841 ctgtccgaat gccaaaatta ttcatgtcga tatcgatcgc gcagagctgg gtaaaatcaa
    36901 gcagccgcat gtggcgattc aggcggatgt tgatgacgtg ctggcgcaat tgatcccgca
    36961 ggtggaagcg caaccgcgtg cagagtggca ccagttggta gcggatttgc agcgcgaatt
    37021 cccgtgtcca atcccgaaag cgtgcgatcc attaagccat tacggcctga tcaacgccgt
    37081 tgccgcctgt gtcgatgaca atgcgattat caccaccgat gtgggtcagc atcagatgtg
    37141 gaccgcgcaa gcttatccgc tcaatcgccc acgccagtgg ctgacctccg gtgggctggg
    37201 cacgatgggt tttggcctgc ctgcggcgat tggcgcggcg ctggcgaacc cggatcgcaa
    37261 agtgttgtgt ttctccggcg acggcagcct gatgatgaat attcaggaga tggcgaccgc
    37321 cagtgaaaat cagctggatg tcaaaatcat tctgatgaac aacgaagcgc tggggctggt
    37381 gcatcagcaa cagagtctgt tctacgagca aggcgttttt gccgccacct atccgggaaa
    37441 aatcaacttt atgcagattg ccgccggatt cggcctcgaa acctgtgatt tgaataacga
    37501 agccgatccg caggctgcat tgcaggaaat catcaatcgc cctggcccgg cgctgatcca
    37561 tgtgcgcatt gatgccgaag aaaaagttta cccgatggtg ccgccaggtg cggcgaatac
    37621 tgaaatggtg ggggaataag ccatgcaaaa cacaactcat gacaacgtga ttctggagct
    37681 caccgttcgc aaccatccgg gcgtaatgac ccacgtttgt ggcctttttg cccgccgcgc
    37741 ttttaacgtt gaaggcattc tttgtctgcc gattcaggac agcgacaaaa gccatatctg
    37801 gctactggtc aatgacgacc agcgtctgga gcagatgata agccaaatcg ataagctgga
    37861 agatgtggtg aaagtgcagc gtaatcagtc cgatccgact atgtttaaca agattgcggt
    37921 gttttttcag taaccgctca aggcttgaac aacatcgcgc ttatcgttaa ggtaagcgcg
    37981 ttttttttta cccgccagga caagaccatg atcaccgttg cccttataga cgatcacctc
    38041 atcgtccgct ccggctttgc gcagctgctg gggctggaac ctgatttgca ggtagttgcc
    38101 gagtttggtt cggggcgcga ggcgctggcg gggctgccgg ggcgcggtgt gcaggtgtgt
    38161 atttgcgata tctccatgcc cgatatctcc ggtctggaac tgctaagcca gctgccgaaa
    38221 ggtatggcga caataatgct ctctgttcat gacagtccgg cgctggttga gcaggcgctt
    38281 aacgcggggg cacgcggctt tctctccaaa cgctgtagcc ctgacgaact gattgcggcg
    38341 gtgcatacgg ttgccacggg cggctgttat ctgacgccgg atattgccat taaactggca
    38401 tccggtcgtc aggacccgct aaccaaacgc gaacggcagg tggcggaaaa actggcgcaa
    38461 ggaatggcgg tgaaagagat tgctgccgaa ctgggcttgt cgccgaaaac ggtgcacgtc
    38521 catcgcgcca acctgatgga aaaactgggt gtcagtaacg acgtagagct ggcgcgccgc
    38581 atgtttgatg gctggtgatg aagacgttgt tctcccgctt aattaccgtt attgcctgct
    38641 tttttatctt ctctgccgca tggttttgcc tgtggagtat cagcctgcat ctggttgagc
    38701 gccctgatat ggcggtgctg ttatttccgt ttggtctgcg tctggggcta atgctgcaat
    38761 gcccgcgcgg atactggccc gtattgctgg gtgcggagtg gctgctgatt tactggctaa
    38821 cgcaggcggt cggtttaacc cattttccgt tattgatgat cggtagttta ctgacgttac
    38881 tgcccgtagc gctgatctcg cgctatcgcc atcagcgtga ctggcgcacc ttgctgttac
    38941 agggggcggc gttaacggcg gcggcgttgt tgcagtcgct gccctggctt tggcacggca
    39001 aagagtcgtg gaatgcgctg ttgctgactt taactggcgg cctgacgctg gccccgatat
    39061 gtctggtgtt ctggcactat ctcgccaata acacctggct gccgctcggc ccgtcactgg
    39121 tttctcaacc gatcaactgg cgcgggcggc atctggtctg gtacttgctg ttgtttgtta
    39181 ttagtctctg gctccagttg ggattgccgg acgaactgtc gcgctttacg ccattctgcc
    39241 tggcgctgcc gattatcgcg ctggcctggc actacggttg gcaaggggcg ctgatcgcga
    39301 cgttgatgaa cgccatcgcg ctgatcgcca gtcaaacctg gcgcgatcat ccggtggatt
    39361 tattgctctc gttgctggtg caaagtctga cagggttgtt actgggcgct ggcatccagc
    39421 ggttgcgtga acttaaccag tcgctgcaaa aggaactggc gcgcaatcag catctggctg
    39481 aacggttgct ggaaaccgaa gagagcgtgc gccgtgatgt ggcgcgtgag ctgcatgatg
    39541 atatcggtca gaccatcact gctattcgta ctcaggcggg cattgttcag cggctggcgg
    39601 cagataacgc cagcgtgaag cagagcgggc agctcatcga acaactatcg ctgggcgttt
    39661 acgacgcggt gcgccgtttg ttgggtcggt tacgtccgcg ccagttggat gatctcaccc
    39721 tggagcaggc catccgctca ctgatgcggg aaatggagct ggaagggcgc ggtattgtca
    39781 gccatctcga atggcgaatc gatgaatcag cattaagtga aaaccagcgc gtgacgctgt
    39841 ttcgtgtctg ccaggaaggg ctgaacaaca ttgtgaaaca tgctgatgcc agcgcggtca
    39901 cgctgcaagg ctggcagcag gatgaacggt tgatgctggt tattgaagac gatggcagcg
    39961 gtttgccgcc gggttccggg caacaaggtt ttggcctcac cggaatgcgc gagcgcgtaa
    40021 cggcgctggg tggcacatta cacatttcct gtctgcacgg cacgcgtgtc agcgtttctc
    40081 tacctcaacg ctatgtctaa ggtttgatga tgttgccgtt tctgaaagcg cctgccgatg
    40141 cgccattaat gactgataaa tatgaaattg atgcccgcta tcgctactgg cgtcggcata
    40201 ttctgctgac catctggctg ggttatgcgc tgttttactt cacgcggaaa agttttaacg
    40261 ccgccgtacc agaaatcctt gctaacggcg tgctcagccg tagcgatatc ggcctgttag
    40321 cgaccctgtt ttacattacc tatggcgtgt cgaagtttgt ctccggcatt gtcagcgatc
    40381 gctcaaatgc ccgttatttt atggggatag ggcttatcgc cacgggcatt atcaacattc
    40441 tgtttggctt ctcgacgtcg ctatgggcgt ttgccgtgct ctgggtgctg aacgcctttt
    40501 tccagggctg gggttcaccg gtgtgtgcgc gtctgttaac ggcctggtat tcacgtaccg
    40561 agcgcggcgg ttggtgggca ttatggaaca cggcgcataa cgtcggcggc gcactcattc
    40621 ccattgtgat ggcagcggct gcactacatt acggctggcg tgccgggatg atgattgctg
    40681 gttgtatggc gatcgtcgtg ggaatttttc tctgctggcg gctgcgcgat cgcccgcagg
    40741 cgttaggttt accggcggtc ggtgaatggc gacacgacgc gctggagata gctcaacaac
    40801 aagaaggggc agggctgacg cgtaaagaga tcctcaccaa atatgtgttg ctgaatccgt
    40861 atatctggct gctttcgttt tgctatgtgc tggtctatgt ggtccgggcg gcgatcaacg
    40921 actggggcaa tttgtatatg tccgagacgc tgggcgtcga tctggtcacg gcgaatacgg
    40981 cagtgacgat gtttgaactg ggtggattta tcggtgcgct ggtagcgggt tggggctcgg
    41041 acaaattgtt taacggcaac cgagggccga tgaatttgat tttcgccgcc ggaattttgc
    41101 tttcagtcgg ctccctgtgg ctgatgccat ttgccagcta cgtgatgcag gcaacctgct
    41161 tcttcaccat tggttttttt gtctttggcc cacagatgtt aatcggtatg gcggcggcag
    41221 agtgttccca caaagaggcg gcaggggcgg cgacggggtt tgtcggcttg tttgcttatc
    41281 tgggggcgtc gcttgctggt tggccgctgg cgaaagtact cgatacctgg cactggagcg
    41341 gattttttgt ggttatctct atcgccgccg ggatttccgc actgctgtta ctgccctttt
    41401 tgaacgccca gacaccgcgc gaagcgtgat gcatctcacc ttttcacttc atatccggca
    41461 aaactaagaa attttccagg ttttgcctgg acgctatctc aggcctgatt tgctgctgat
    41521 ttttacaatg catgcctcac gcaggtattc atttcaggag taacccatgc tggctttctt
    41581 aaaccaggtt cgcaagccga ccctggacct tccgctcgaa gtgcggcgca aaatgtggtt
    41641 caaaccgttc atgcaatcct acctggtggt ctttatcggc tacctgacga tgtacctgat
    41701 tcgcaagaac tttaacatcg cgcagaacga catgatttcg acctacgggt tgagcatgac
    41761 gcagctgggg atgatcggcc tgggtttctc catcacttat ggcgtgggta aaacgctggt
    41821 ttcctactac gccgacggca aaaacaccaa acaattcctg ccgtttatgc ttatcctgtc
    41881 tgccatttgt atgctgggct tcagtgccag tatgggcagc ggctcggtta gcctgttcct
    41941 gatgattgcc ttctacgcct taagcggctt tttccagagt accggcggtt cgtgcagtta
    42001 ctccaccatc accaaatgga cgccgcgtcg taaacgcggg acattcctcg gtttctggaa
    42061 tatttctcac aaccttggcg gtgcaggcgc agcaggtgtg gcgctgttcg gggcaaatta
    42121 cctgttcgat ggccatgtca tcggcatgtt tatcttcccg tcgattatcg cgctgattgt
    42181 cggttttatc ggcctgcgtt acggtagcga ctccccggaa tcttatggcc tcggcaaagc
    42241 tgaagaactg ttcggcgagg agatcagcga agaggacaaa gagacagaat ctaccgatat
    42301 gaccaagtgg cagatctttg ttgagtatgt gctgaaaaac aaagtgatct ggctgctgtg
    42361 cttcgccaac attttcctct atgtggtacg tattggtatc gaccagtggt caaccgtata
    42421 cgcgttccag gaactgaaac tctctaaagc ggtggcgatt cagggcttta cgctgtttga
    42481 agctggtgcg ctggtcggta cgctgctgtg gggctggctc tctgacctgg cgaacggtcg
    42541 ccgtggcctg gtggcctgca tcgcgctggc gctgattatc gccacgctcg gtgtgtatca
    42601 acatgccagc aacgaatata tctatctggc ttctctcttt gcgttgggtt tcctggtctt
    42661 tggcccgcaa ttgttgattg gtgtggctgc tgttggcttt gtacctaaaa aagcgattgg
    42721 cgctgccgat ggtattaaag gcacctttgc ttacctgatt ggtgacagct ttgccaagtt
    42781 aggtctggga atgattgccg atgggacgcc ggtattcggc cttaccggct gggcaggcac
    42841 cttcgccgca cttgatatcg ccgcgattgg ttgtatctgc ctgatggcga tagtggcggt
    42901 gatggaagaa cgcaaaatcc gccgcgagaa aaaaattcag cagttgacag tggcataaac
    42961 gtaactggtg acttttgccc ggcatgacgc cgggcttttt tattattccg tgacttccag
    43021 cgtagtgaag gcaaacttct cgccatcaaa tagcccctga ctggttagtt ttagcgcggg
    43081 gatcactggc agagaaagaa acgccatctg aataaacggc tcatcgggta acggaccgca
    43141 ttcacgggcg gcggctttca aggcgtcaat ttgttccgcc agcaactgcg ccgtgtcggt
    43201 gctcatcagg ccggcaatgg gcaacggcag atgactttgg acctggccgt tacgtaccac
    43261 gcacagcccg ccgccatcct gaatcacctg attgaccgcc agcgccatct cttcggcact
    43321 gcgaccgatc accacaatat tatggctgtc atggctgacc gtcgcagcca gcgcaccttc
    43381 attcagtcca aagccgccaa gcaaaccaca agccggagcc agccgttgcc cgtaacgttc
    43441 aagtacggca ataaagctca catcatcgcg atcaaaacca ttttcgctgt agacgctgga
    43501 gtgggagtgc gtaatcaatt cgttatggat gacgtcaatg acccgatagc gttttccggg
    43561 cgtaaattgc agggcaaagt cgctggcgga aactggctgg cgggcaatgg tgttgccata
    43621 tggcggagcg gattgtgcca gtctcgccga ctcttccgcc tgtaaggttt gcgcatcaat
    43681 cggctcgcct ttcaccagta cctgctgcac cgtgacctta cgcgcatcgc tcaacaggac
    43741 gatatcggcc tgcttgccgg gtgccagtaa gccgaggtga ttcagaccaa agtggcgcgc
    43801 cgtcgaccag ctggcgacgc gatatgccac atgcagcggc acattgtgtt gttcgatcag
    43861 gcggcgaatt aaggcatcga tgtgtccttc atgggcgatc tcccacgggt tacggtcatc
    43921 ggtacagagc atgcattgcg ggctgttaaa ttcgttgatc aacggtgcca gcgcgttgag
    43981 attgcgggca gcggaccctt cgcggatcat caacgacatg ccgagttgta atttccggcg
    44041 tccttcttcc agctgataac tttcgtggca gttttcaata cccgcagtaa tataggcgtt
    44101 aagttcttta ccacccaaac ccgggcagtg accgtccagc gtcaggtggc gaaatgcatc
    44161 cagtttatcg agcagcgcat tctgcccgct aattacgcca gggtagtcca tcatttctgc
    44221 aaggccggta acctgcggat ggtcccgcca ggcgagcatc tgttcaaggg taaaactggc
    44281 accgttaaca tcgcagcctt ccagggcggg tacgcaagag ctgacctgta agtactggtt
    44341 ttgccttgcc tgttcggcac agcgggcaaa ccaggcgaat ccggcttctc ccatcacgtt
    44401 gacgatttca tgagggtcgc aaataacggt cgtcaggccg cgcggcaggg tagcggtttc
    44461 aaaagtgacc ggcgtcatca tgctggattc aatatgcagg tgagcatcaa taaaccctgg
    44521 caccgccgtt gcgccgcgag catcaatccg ctgcaaagcc ggagcatcag cgtattctgc
    44581 gccaacaccg gcaatgtaac gtcctttaat cacaattggg ccggaaattt ctccgccatt
    44641 gatcaggtcg agaatagaga cattatcaat aatataatcg gcaacagcgt cgccacggga
    44701 aacggctaac aattcctggt attcagcccg g
//
LOCUS       DAPHJE010000025        44136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPHJE010000025 DAPHJE010000000
VERSION     DAPHJE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.025"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(69..539)
                     /locus_tag="RH222_001391"
     CDS             complement(69..539)
                     /locus_tag="RH222_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV3796441.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(586..1305)
                     /gene="yehT"
                     /locus_tag="RH222_001392"
     CDS             complement(586..1305)
                     /gene="yehT"
                     /locus_tag="RH222_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV3796442.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1302..2987)
                     /gene="btsS"
                     /locus_tag="RH222_001393"
     CDS             complement(1302..2987)
                     /gene="btsS"
                     /locus_tag="RH222_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV3796443.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3209..3940
                     /gene="mlrA"
                     /locus_tag="RH222_001394"
     CDS             3209..3940
                     /gene="mlrA"
                     /locus_tag="RH222_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV3796444.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4000..4107
                     /locus_tag="RH222_001395"
     CDS             4000..4107
                     /locus_tag="RH222_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV3796445.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4088..4819)
                     /gene="yehW"
                     /locus_tag="RH222_001396"
     CDS             complement(4088..4819)
                     /gene="yehW"
                     /locus_tag="RH222_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV3796446.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4824..5750)
                     /gene="yehX"
                     /locus_tag="RH222_001397"
     CDS             complement(4824..5750)
                     /gene="yehX"
                     /locus_tag="RH222_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV3796447.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERADGEALAEEMTLRDALS
                     LFVARGCEVLPVVNIQGQPCGTLHFQDLLVEA"
     gene            complement(5743..6900)
                     /gene="yehY"
                     /locus_tag="RH222_001398"
     CDS             complement(5743..6900)
                     /gene="yehY"
                     /locus_tag="RH222_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV3796448.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTVFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(6907..7824)
                     /gene="osmF"
                     /locus_tag="RH222_001399"
     CDS             complement(6907..7824)
                     /gene="osmF"
                     /locus_tag="RH222_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV3796449.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(8035..10332)
                     /gene="bglX"
                     /locus_tag="RH222_001400"
     CDS             complement(8035..10332)
                     /gene="bglX"
                     /locus_tag="RH222_001400"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV3796450.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10528..12243
                     /gene="dld"
                     /locus_tag="RH222_001401"
     CDS             10528..12243
                     /gene="dld"
                     /locus_tag="RH222_001401"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV3796451.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12281..13213)
                     /gene="pbpG"
                     /locus_tag="RH222_001402"
     CDS             complement(12281..13213)
                     /gene="pbpG"
                     /locus_tag="RH222_001402"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV3796452.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKAIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13387..13974)
                     /locus_tag="RH222_001403"
     CDS             complement(13387..13974)
                     /locus_tag="RH222_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV3796453.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14144..14722
                     /locus_tag="RH222_001404"
     CDS             14144..14722
                     /locus_tag="RH222_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3796454.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(14852..15613)
                     /locus_tag="RH222_001405"
     CDS             complement(14852..15613)
                     /locus_tag="RH222_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3796455.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15666..17102)
                     /gene="mdtQ"
                     /locus_tag="RH222_001406"
     CDS             complement(15666..17102)
                     /gene="mdtQ"
                     /locus_tag="RH222_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV3796456.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17326..17409
                     /gene="yohP"
                     /locus_tag="RH222_001407"
     CDS             17326..17409
                     /gene="yohP"
                     /locus_tag="RH222_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDV3796457.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(17780..18727)
                     /gene="dusC"
                     /locus_tag="RH222_001408"
     CDS             complement(17780..18727)
                     /gene="dusC"
                     /locus_tag="RH222_001408"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV3796458.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            18966..19364
                     /locus_tag="RH222_001409"
     CDS             18966..19364
                     /locus_tag="RH222_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV3796459.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19361..20056
                     /locus_tag="RH222_001410"
     CDS             19361..20056
                     /locus_tag="RH222_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV3796460.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            20186..21070
                     /gene="cdd"
                     /locus_tag="RH222_001411"
     CDS             20186..21070
                     /gene="cdd"
                     /locus_tag="RH222_001411"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV3796461.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVTLECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            21220..21939
                     /gene="sanA"
                     /locus_tag="RH222_001412"
     CDS             21220..21939
                     /gene="sanA"
                     /locus_tag="RH222_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV3796462.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            21942..22181
                     /locus_tag="RH222_001413"
     CDS             21942..22181
                     /locus_tag="RH222_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV3796463.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            22500..23738
                     /gene="preT"
                     /locus_tag="RH222_001414"
     CDS             22500..23738
                     /gene="preT"
                     /locus_tag="RH222_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV3796464.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKMLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            23732..24967
                     /gene="preA"
                     /locus_tag="RH222_001415"
     CDS             23732..24967
                     /gene="preA"
                     /locus_tag="RH222_001415"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV3796465.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(25038..26048)
                     /gene="mglC"
                     /locus_tag="RH222_001416"
     CDS             complement(25038..26048)
                     /gene="mglC"
                     /locus_tag="RH222_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV3796466.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(26064..27584)
                     /gene="mglA"
                     /locus_tag="RH222_001417"
     CDS             complement(26064..27584)
                     /gene="mglA"
                     /locus_tag="RH222_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV3796467.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(27645..28643)
                     /gene="mglB"
                     /locus_tag="RH222_001418"
     CDS             complement(27645..28643)
                     /gene="mglB"
                     /locus_tag="RH222_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV3796468.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(28923..29963)
                     /gene="galS"
                     /locus_tag="RH222_001419"
     CDS             complement(28923..29963)
                     /gene="galS"
                     /locus_tag="RH222_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV3796469.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(30105..31262)
                     /locus_tag="RH222_001420"
     CDS             complement(30105..31262)
                     /locus_tag="RH222_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV3796470.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(31279..31947)
                     /gene="folE"
                     /locus_tag="RH222_001421"
     CDS             complement(31279..31947)
                     /gene="folE"
                     /locus_tag="RH222_001421"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV3796471.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            32205..33041
                     /gene="yeiG"
                     /locus_tag="RH222_001422"
     CDS             32205..33041
                     /gene="yeiG"
                     /locus_tag="RH222_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV3796472.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(33073..35064)
                     /gene="cirA"
                     /locus_tag="RH222_001423"
     CDS             complement(33073..35064)
                     /gene="cirA"
                     /locus_tag="RH222_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDV3796473.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSFNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(35358..36827)
                     /gene="lysP"
                     /locus_tag="RH222_001424"
     CDS             complement(35358..36827)
                     /gene="lysP"
                     /locus_tag="RH222_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV3796474.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(37032..37913)
                     /locus_tag="RH222_001425"
     CDS             complement(37032..37913)
                     /locus_tag="RH222_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3796475.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            38012..39061
                     /locus_tag="RH222_001426"
     CDS             38012..39061
                     /locus_tag="RH222_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV3796476.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            39135..39992
                     /gene="nfo"
                     /locus_tag="RH222_001427"
     CDS             39135..39992
                     /gene="nfo"
                     /locus_tag="RH222_001427"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV3796477.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            39995..41083
                     /locus_tag="RH222_001428"
     CDS             39995..41083
                     /locus_tag="RH222_001428"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3796478.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(41139..42389)
                     /locus_tag="RH222_001429"
     CDS             complement(41139..42389)
                     /locus_tag="RH222_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV3796479.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(42489..43430)
                     /gene="rihB"
                     /locus_tag="RH222_001430"
     CDS             complement(42489..43430)
                     /gene="rihB"
                     /locus_tag="RH222_001430"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV3796480.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            43560..>44136
                     /gene="yeiL"
                     /locus_tag="RH222_001431"
     CDS             43560..>44136
                     /gene="yeiL"
                     /locus_tag="RH222_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV3796481.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQ"
BASE COUNT        10783 a        11768 c        10968 g        10617 t
ORIGIN      
        1 aagccgctgg ttttaaagaa gtgaaataat cggttggcgg tcatgctcta aacatgaccg
       61 ccaatttttt agcctttttt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
      121 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcacgcat catcgctgta
      181 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
      241 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
      301 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccgttgagg
      361 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
      421 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
      481 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
      541 aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
      601 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
      661 caactcggcc tggccattat cttccagacg aatctcctgt aaatgcgcga gattaaccag
      721 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
      781 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
      841 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
      901 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
      961 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     1021 agcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcggtgagaa aaacaatata
     1081 cggacgatgt tccggatcga gcatccccac catttccaga ccactgatgc gcggcatctg
     1141 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     1201 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cgcgcaggtt
     1261 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     1321 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tatcccatag
     1381 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     1441 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     1501 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     1561 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     1621 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     1681 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     1741 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcga
     1801 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgggggtt cacctgggcg
     1861 tgaagcagtt tgatctctga ctgggtgagc atcgcttttt gccgctcata ttgcccggcg
     1921 aggatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt gattgaactg
     1981 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     2041 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     2101 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     2161 gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     2221 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     2281 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     2341 gccactttca gcgcagtggc agaaaaagca gaagtgtatt tttcaaacat cgcgcgttta
     2401 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggtgca
     2461 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc aaggatgatc
     2521 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     2581 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     2641 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     2701 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     2761 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     2821 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     2881 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     2941 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     3001 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     3061 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     3121 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     3181 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     3241 gctttgtgat attaaccctg tcacgttacg cgcgtggcag aggcgttacg ggttgctgaa
     3301 accgcaacgg acagacggcg gtcatcggct gttcaacgat gccgatattg accgtatccg
     3361 cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc aaagttaaaa tgctgctcag
     3421 taatgaaaat gttgatgtgc agaacggctg gcgcgatcag caagaaacat tactgaccta
     3481 cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg gtcaggatta
     3541 ccccgcccag acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca
     3601 acaaccgact ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc
     3661 catttgtctg gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa
     3721 tattcaggat accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg
     3781 cattgatgtc ctcgcccact cgctcaatca actacgccct gaactattcg aaggccgtac
     3841 attgctggtg tggtgcggtg aaaatcgaac ctccgcccaa cagcagcaac tcaccagttg
     3901 gcaagaacaa ggccatgata ttttcccact cggcatttaa tgattcgtta acaaatgcgc
     3961 tttactgtac aatcctttcg ttaacataag gagtgcaata tgcgcatagc taaaattggg
     4021 gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat gctcgcaggt
     4081 tataccttta ttttgcgtgc tggctaagcg cctgcaccag cctttcaaac agtcggtctg
     4141 cgatgatcgc cgccagtgcc accagtaacg ccccctggat cacatacgcg gtattaaatc
     4201 cgctaagccc gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt gaggcgatcg
     4261 tcgccgtacc aatgttgata atcaccgaag ttcgcacgcc cgccagaatc accggagccg
     4321 ccagcggtag ctcgacctta cgcagtcgct gacctcgact cattcccata cctttcgcaa
     4381 cttctgtcac gctggcatca atcgctccca gcccggcaag tgtcgcctgc aggacgggca
     4441 gcacaccgta aaggatcaag gcgataatcg ctggttgcag accaaagccg atcaccggaa
     4501 cggcaatcgc cagcactgcg acaggcggaa aagtctgtcc aacggcggca atagtttcca
     4561 ccagtgggcg aaattccgcg ccccacgggc gagtgacagc aattccggca ccagtgccaa
     4621 tgatcaccgc aaacaaactc gaaattccca ccagccagaa atgagccagt gccagagctg
     4681 caaaactttc ttgctgataa acgggtcgtg gcagttgtgg gaacaaggca gcaaacagcg
     4741 gctggctgta aggcagccag aaaatcagtg ccacaaacaa agcaatgagc cagaacagcg
     4801 gatcgcgcaa catcttcata cgcttacgcc tccaccagca gatcctgaaa atgcagcgtg
     4861 ccgcaaggct ggccctggat gttcaccacc ggcagcacct cgcatccccg cgcaacaaac
     4921 agagagagcg catcgcgtag cgtcatctct tctgccagtg cctcaccatc tgcccgttct
     4981 tcgcgacgca cgtaatccgc cacactacgt aacgaaagca ggcgcacacc cagttcacta
     5041 cgtccaaaaa actggcggac aaaatcattc gccggacgag tcagcatcgt cagcggattg
     5101 ccctgctgca ccacttcacc gtgatccatc aataccagat gttctgccag ccgtagcgcc
     5161 tcatcaatat catgagtgac cagcacaata gtgcgcccca gcaaacggtg aatgcgcgtc
     5221 atctcttgtt gcaacgcgcc gcgcgttacc gggtccagtg cgccaaaagg ttcatccatc
     5281 agtaagactt gcggatcggc agccagtgcg cgcgccactc ccacacgttg ctgctgacca
     5341 ccggaaagct gatgcggata acgctcacgc aaatttgact ccagccccag cagcgccatt
     5401 aattcgtcga tacgatcgtc aatccgcgcc cgcgaccatt tttgtaattg cggcacggta
     5461 gcgatgtttt gcgccacgct ccagtggggg aacaggccaa tagattgaat ggcatagccc
     5521 atccggcggc gcaactccag tactggcagc gagcgaattt cttctccggc aaagcggatc
     5581 acgccgctgt catgctccac caggcggtta atcattttca gagtggtgga tttgccggag
     5641 cctgatgtac caatcagcac cgaaaaactc ccttcctgaa aattgagatt gagatcgtta
     5701 acggcttttt gtgcgccgaa cagtttgctg acatggctaa attcaatcat tacgtttcac
     5761 cttcagcagt gcgataagca aatcgaacag cgcgtcggta agcaccgcca gaacaattac
     5821 cgggatcacc cccagcaaca ctaaatcaat ggcgctgctt agcagcccct ggaaaaccag
     5881 cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc gccataccta cagtttgcac
     5941 catcaccacc cgcaggctgc gcagaaatac cggtaacgcc agtggtaact gaacatgcag
     6001 gaatcgctgc gccccgctca tgcccatcgc tctggcgctc tccagcacat cgcgcgggat
     6061 ctggttcaaa ccgactacca cgccgcgcac cagcggcagc aaggcataga gcaccagcgc
     6121 aatcagtgcg ggtgtcattc cggttcctgc tatgccgagc gtccccagcc acggaaagac
     6181 cgtaaccagc gcggcaagcg gcgcaatcaa caggccaaag agcgccaccg aaggcacggt
     6241 ctgaatgaca ttgagcagag aaaaaattgc cccctgccgc gcagtggaaa agtagcacca
     6301 gatgcccaac ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca gcaacgtcag
     6361 atgttgtgcc agcgcgtcgt caaacacatc ctgacggttg gcgtattctt tcattagtga
     6421 gagatcgtta agcgtgccgg agtacagcaa ccacagcgga ataatggcaa tctgcatatg
     6481 caacaaccag cgccacagcg gatgcgtgga gattcggcgg atggcatcgc tacaggccag
     6541 caatgccagc gccgcagcca gccagaaacc actgccgagg ctggtacgcg ccagcgcact
     6601 gccattttgc gccagttgtg tcgccgcctt tcccgcgccc cacaccagca atacgaagac
     6661 gaattgcgcc agaatgagtg cacaaatgct gcctttttta ccgggaataa aacaggccgt
     6721 cagccaggcg caaccaacgc ccaccagcat ccagatcgtt tgcggccaca gctgccagag
     6781 atgacgcccc tcacccgaaa ctaaacgatt aggcgcgtaa ctgataaacg gcagcgccgc
     6841 tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga ttaatacgga aataagtcac
     6901 gggaaattac ttcgtccacc ctttttgttt caggtagtcg gcagccactt ttttggcatc
     6961 cagtccttcc actgcaatgc tggcattcag ttgctgcaat gtttttgcat cgaggctggc
     7021 gaagactggc tgtagccact gtgccatttg cggatactcc ttcaacaccg attcacgcac
     7081 cactggtgca ggcgcataga taggttgcac gccttgcgga tcgcttaagg tttgcagccc
     7141 cagcgccgct accgagccgt cagtgccgta agccattgca gcattaacgc cagaggtttg
     7201 ctgggcagcg gctttgatcg tcaccgccgt gtcgccacca gccagtgaca gcaactgatc
     7261 ctgaccgagc ttaaagccat aggctttttc aaacgcgggt aacgcatcgg cgcgttcgat
     7321 aaactctgcc gaggctgcca gtttgaaggt gccgccctct tgcagataac gactcaggtc
     7381 ggcaagcgaa gtgagtttat ttttctctgc cacatcctga cgcacggcga tagtccaggt
     7441 gttatttgca ggcgcgggcg tcagccagat taacttgttg tgctccgaat cgagcttttt
     7501 gactttctcg taaccttgct gcgcgttttt ccacgctgca tcgttttcat ctttaaagaa
     7561 gaaagcgcca ttgccggtat attccggata gatatccagt tcaccggaag taatcgcccc
     7621 gcgcaccaca ggagtcgttc cgagttgcac tttattcacc gtcggtacgc cgtggctttc
     7681 cagcacctgc aaaatgatat tgccgagtag cgcaccttcg gtatcgattt ttgaaccgac
     7741 tttaacgggg gaagccgcct gtagcggcag gctcacggct gccaacataa ccagtgaacc
     7801 tgcccagagc tttaagagtg gcatgatgct ttcctcattc ttttactgtt gttttcagcg
     7861 aattaagaga aaagcatagt tgataatggc ggggttagct tgagcggaag gattcagttg
     7921 cagaatcaga taaatactta agaggcatat tcggtccggc gtttcctgcc ggatgcggcg
     7981 cgagcgcctt atccggccta caaagggcgc aaacgtcgcg cccttactaa agcattacag
     8041 caactcaaac tcgcctttct taacgcgtgc ggaatcagtg ccgataaata cattgaactt
     8101 gccaggctcg gcgtcatatt tcatctgttg attccagaac ttcagcgcct caatatcgat
     8161 cgggaagctg acagtctgag tttcgcccgg tttcagggtg attttctcaa agcctttcag
     8221 ctgtttcaca gggcgactca tggaagccgt cacatcctgc aagtacatct gcactaccgt
     8281 ggccccctcg cgcttaccgg tgttcgtcac ctgcacgctg gcagtcactt tgccgtcacg
     8341 cttcatggtc ggcgcagaaa gtttcacatc agagacggtg aaagtggtgt agctcagccc
     8401 atagccgaac ggatacaacg ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt
     8461 cggcttgtcg gcattatacg ggcgaccggt attcagatgg ctgtagtaca ccgggatctg
     8521 cccgacagaa cgcgggaagg acattggcag cttgccggac gggttgtaat cgccaaacaa
     8581 tacatcggca attgcattac cgccttcagt ccccgcaaac caggtttcca gaatcgcatc
     8641 agcctgctga tcttctttca ccagcgccag cggacgcccg ttcatcagca ccagcaccag
     8701 cggtttaccg gtggctttca gcgccgcaat caagtcacgt tggctttgcg gaatagtgat
     8761 atcggtccgg ctggaggctt cgtgcgccat cccctgtgct tcaccgacta cagccaccac
     8821 cacatcagat tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg gcgaacgcgg
     8881 atcgactttg accgcttctt catactgatt caggaaatcg ataatgcctt tgtcactggt
     8941 aacgttcgcc cctttggcat acagcacttt accgttttca ccgacggaat ttttaatccc
     9001 ggtcagtacg gtcacggatt gatcggcaac accggctgcg gaccagctgc ccatcacgtc
     9061 acgtttactg tccgccagtg gcccaaccac cgcaatggtg gccgattttt tcagcggtaa
     9121 cgtttcgaga cggtttttca gcaacaccag gctttcgcgc gccacttcac gcgcttcttt
     9181 acggtgcagg cggctttcgg cattggtatc caccgggtca gactctttcg gccccaaatg
     9241 gctgtatggg tcgttaaaca atcccatatc atatttaacg ttcagtacat ggcgggcagc
     9301 atcgtccagc tcttccatcg tcactttgcc ggatttgatc aacccaggca gatacttcga
     9361 gtaatactcg tcgctcatac tcatgttgat tccggatttc agcgccacgc gcaccgcatc
     9421 ttccgggtct gccgccgtgc catgtttaat cagctctttg attgcaccgt gatcggaaac
     9481 ggtgatgcct ttaaagcccc actggtcgcg cagaacatct ttcagcagcc aggaatcgga
     9541 ggtggctggc gtgccgttca gcgagttcag cgccaccatc accgcgccgc tgcctgcgtc
     9601 cagccccgct ttgtacggcg gcatataatc attaaacagg cgctgcggac tcatatcgac
     9661 ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt
     9721 catcaccgag tagcgatctg ccgggctttt accctgcatc gcttccacca tggttttacc
     9781 cattgttgag gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg
     9841 atcgcgcgag acatcgacca tcggtgccca ggtcatattc aggccatcat ctgccgcttc
     9901 ataagcagag acacgtccga ccgttttcac tgcatcgagg ttaaaagacg aggccagacc
     9961 gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg
    10021 aattttcagg cggcttaatt ccatcacctg atcctgcatg gcgcggatat cctgacgggt
    10081 tacggtgttg aaaatcgccc caacctgacc gtctttgatc atctcgcgga tcgcctcttt
    10141 cgggttatcc gggccgacgc tgattaagcg cagctgacca attttctcat caactgtcat
    10201 tttcttaagc agttcggtga cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc
    10261 gaataaatca tccgccagtg ctggctgcag ggccagactc accgcgattc ctactgaaca
    10321 tagccatttc atatggattt ttcttcctct gttgcccgac gaggcagcga aaatggtgca
    10381 aaaaccgtag tttgccataa gcatgatgga gagagaaaaa gaatgctcag tttattgtct
    10441 gaattttcaa aatattcact cgctgaattg tcatacaagg cgctattcta gtttgtgata
    10501 ttttttcgcc accacaagga gtggaaaatg tcttccatga caacaactga taataaagcc
    10561 tttttgaatg aacttgcccg tctggtcggt cattcacacc tgctcaccga tcccgcaaaa
    10621 acggcccgct atcgcaaggg cttccgttct ggtcagggcg acgcgctggc tgtcgttttc
    10681 cctggctcac tactggaatt gtggcgggtg ctgaaagcct gtgtcaccgc cgacaaaatt
    10741 attctgatgc aggctgccaa tacaggcctg accgaaggat cgacgccaaa cggtaacgac
    10801 tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg acaagctgca cgttcttggc
    10861 aagggcgaac aggtgctggc ctatccgggc accacgctct attcgctgga aaaagccctc
    10921 aaaccgctgg gacgcgaacc gcactcggtg attggatcgt cgtgtatagg cgcatcggtt
    10981 atcggcggta tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc
    11041 gaaatgtcgt tattcgcgcg tataaatgaa gacggcaaac tgacgctggt gaaccatctg
    11101 gggattgatc tgggcgaaac gccggagcag atccttagca agctggatga tgatcgcatc
    11161 aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg attatgacta tgtccaccgc
    11221 gttcgtgata ttgaagccga cacgcccgca cgttataacg ccgatccgga tcggttattt
    11281 gaatcttctg gttgcgccgg gaagctggcc gtctttgcgg tacgtctaga taccttcgaa
    11341 gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc agccggaagt gctgaccgaa
    11401 atccgccgtc atattctggc taacttcgaa aatctgccgg ttgccgggga atatatgcac
    11461 cgggatatct acgatattgc ggaaaaatac ggcaaagaca ccttcctgat gattgataag
    11521 ttaggcaccg acaagatgcc gttcttcttt aatctcaagg gacgcaccga tgcgatgctg
    11581 gagaaagtga aattcttccg tccgcatttt actgaccgtg cgatgcaaaa attcggtcac
    11641 ctgttcccca gccatttacc gccgcgcatg aaaaactggc gcgataaata cgagcatcat
    11701 ctgctgttaa aaatggcggg cgatggcgtg ggcgaagcca aatcgtggtt ggtggattat
    11761 ttcaaacagg ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag caaagcgttt
    11821 ttacaccgtt tcgccgctgc gggcgcagca attcgttatc aggcggtgca ttccgatgaa
    11881 gtcgaagaca ttctggcgtt ggatatcgct ctgcggcgta acgacaccga gtggtatgag
    11941 catttaccgc cggagatcga cagccagctg gtgcacaagc tctattacgg ccattttatg
    12001 tgctatgtct tccatcagga ttacatagtg aaaaaaggcg tggatgtgca tgcgttaaaa
    12061 gaacagatgc tggaactgct acagcagcgc ggcgcgcaat accctgccga gcataacgtc
    12121 ggtcatttgt ataaagcacc ggagacgttg cagaagttct atcgcgagaa cgatccgacc
    12181 aacagcatga atccggggat cggtaaaacc agtaaacgga aaaactggca ggaagtggag
    12241 taaaaattac ggatggcaga gtatcgccat ccgaattcac ttaatcgttc tgtgccgtct
    12301 gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggca
    12361 caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg
    12421 tatatttgcc aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtac
    12481 gcatcaccag acaatggccc gccgcattgg taaagccggt tttggttaac tgaatattcc
    12541 agttatcgcg atacaccaga tgattagtat tgcggaacgg cagcgtatac gtcgggttag
    12601 agaaggttgc catatcttcc cgggtagtac ttaactgccc gatcaacgga tattgtttgc
    12661 tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc gacaatccgg
    12721 taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcattgcct
    12781 taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc gcgcggtttt
    12841 ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta ttcagtcgta
    12901 cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt agtttttcat
    12961 ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa
    13021 tcggacgcac cagatccggg tggttcgaat agatcgcttt gttggtattc agatcaacaa
    13081 tcatcgcgct accggaggca atttccggtt gtgaagcggt ggtagcggct gccgttttcg
    13141 caacggcctg cggtgcaaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc
    13201 gaaatttcgg catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg
    13261 gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa
    13321 aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc aacgtcagag
    13381 aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg cgagcagcac
    13441 ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc cttcctcttt
    13501 gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt aaaacagcgc
    13561 caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca gaccgcttaa
    13621 aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg
    13681 cggataatta cgcgccatcc accagatgac ccgccccatc accgcgaccc cagccagcat
    13741 aacgccgtaa aacaagacag ccagcgccag tccggtaaac caggataact tcaggatagt
    13801 gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat
    13861 cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt tttcgcgatt
    13921 aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc
    13981 cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt
    14041 tgttcgccca tgtaattttc actgtctgat gaacgtcctt tttcagcgtg aatgattcac
    14101 agggtgtatg cttactggca accaaaggga gacagactgg cctatggatc tcaatacact
    14161 tatctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt
    14221 gactttgctg ggaggcgttg cggcgcatca ggggctatta aagttcccgc tggtggtact
    14281 ttctgtggcg cttggcggca tgattggcga ccaggtgctc tatctgtgcg ggcggcggtt
    14341 tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa
    14401 acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt atggctttcg
    14461 ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccgct
    14521 gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt acgctggtgg
    14581 tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact gggtctggtt
    14641 gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc gcgggaagaa
    14701 aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg ccgcatccga
    14761 catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta ccaaaatttt
    14821 acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc ggattcgcca
    14881 acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg ccctctgagc
    14941 acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc agaggaatcg
    15001 aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc gtggcgatcg
    15061 ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc gccatcgttt
    15121 tggttaaccc gccgagcgca tgtttagcgg ctgtgtaagc gctggcatcc ggcagcggcg
    15181 tatgttcatg taccgacgta atgttgatga tgcgaccgcc ctgcccttgt ttcaccatct
    15241 ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta aaaatcttgc
    15301 gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc gcattattca
    15361 ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc gcaccttctg
    15421 gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta actacctcac
    15481 gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata tcaaacccct
    15541 gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg
    15601 caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca
    15661 tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga agcatccagc
    15721 cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg
    15781 ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag acctaccaca
    15841 cgcaaggcat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag tgtctgaacc
    15901 tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggcg
    15961 atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg accactatcg
    16021 aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg acggaacaga
    16081 tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc aggataaaat
    16141 gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg cgccgcctgc
    16201 aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg gcttgccact
    16261 ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggttatttgt aagtgcgctt
    16321 aaccgtgcct caataatttt cattttcccc gcgacatcgt tgagctgctg ccgggttttg
    16381 ctggcattaa tatcggtttc cacaccttca actgaagaag taatcccgtt ctgatatagc
    16441 tggcgatcgg tcgcgataat ggtgttctgc tctttttcta tttgctgcaa gaccgtgttt
    16501 aacgccgcct gggtttgcca ctcccagtac aggcgggcta cgctgccagc cagcaattgg
    16561 cgggtttgct cgcgttccgc cgcccgtgct ttaaccgtac ccaggcgggc agtaacctcc
    16621 gcccgattct ttccccagat atcgagatgc cagcccgccg ttaagccaaa agttccgttg
    16681 gtgtaccacg gaccggtcgt acctgcggcc ggatcgttca gagcaaacgg ccccattaag
    16741 ccttctgccg acattttttg ccgctccata tccgcagaaa agtcgatctg cggaccatcc
    16801 tgagtagcaa ttgccttcgc ctgggcttca gctagctgaa tgcgctgttc agccacctgc
    16861 atatccggtg cgttctgtag tgcattgtta attaaggaag tgagttgatt atcgtgatac
    16921 tccagccacc attggctgtc tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca
    16981 acttgtgcag caggcgtttg ctggcttaac gcctggcggg tttcatgcat aggtgcacac
    17041 ccggccagca tcagtaacag cggaaaacag gcgatggctg gataaaagga atcacgattc
    17101 atgggggaat aatcaggtaa gaaaaggtgc gcggagatta ccgtgtgttg cgatatattt
    17161 tttagtttcg cgtggcaata catcagtagc aataaaacga catatccaga aaaatataga
    17221 ctaagtaaat gatatcttcc gatttatctt aatcgtttat ggataacggc aaagggcttc
    17281 gttttttcct atacttattc agcactcaca aataaaggaa cgccaatgaa aattatactc
    17341 tgggctgtat tgattatttt cctgattggg ctactggtgg tgactggcgt atttaagatg
    17401 atattttaaa attaattaat gtcatcaggt ccgaaaataa cgagaatatt tcagtctctc
    17461 atcctgttgc gctcctgtca tgtgcattgc ttcatataat cactggcgca aggagcgcgc
    17521 agggcggcca atcgccgccg ccccctgcac ccccgggctc tggcgaacaa aatcgccgct
    17581 gcgcggtgcc ctcgacttat cccttacggc taccgggtcg ggcgcgaggt aacatccctg
    17641 taaaacgcgc cctcagccca catccatgtg ggctgccccg gccttcaggg aacgcctcgg
    17701 caattttgac gccaccaaac aaccgtgcgg cctattgata aagagctaac acattgtcaa
    17761 aaaacatcac tatggttttt tagagtttct cgatatcaat tgcctgaata gcccttgcaa
    17821 tatcagggga attattcaac acccgaacat gctgaaataa ttccgttgct tcatcgtatt
    17881 ctttacgcaa ataactcaac cactgtttaa tccgcgcaac gtgatataac ccggtatcgc
    17941 cctgcttttc cagacgggta tatttttgca gcaaagcaac cacctccggc cacggcattc
    18001 gcggttcgtt atattttact acccggctca ggttgggaat attgagcgcc ccgcgaccaa
    18061 tcatcactgc gtcgcagccg ctgatcgcaa tgcattgttg cgcgctctgc cagtcccaga
    18121 tttcgccgtt ggcaatcacc ggaatattca gccgctggcg aatctcgcca atcgcctgcc
    18181 agtcaatatg ctccgcgcgg taaccctgct cttttgtccg tccatgcacc accagctccg
    18241 tagcgccagc ctgttgaacc gcatcggcga tttcaaattt cttctcaccg ctgtcccagc
    18301 ccagacgcac ttttacgctg acgggcaaat gcgctggtac agcttcacgc atcgcttttg
    18361 caccctggta gatgagttca ggatctttga gtaacgtcgc gccgccgccg ctaccgttaa
    18421 ccgttttcga cgggcagccg caattgagat ccacgcccca ggaacctaac tccaccgcac
    18481 gggcggcgtt ctctgccagc cattgtggga actgacctaa caactgcacg cgcaccagcg
    18541 taccagatgg tgtccggctg gcgttttgta gctcagggca aatgcgatga aagactttta
    18601 ccggcagcag ttgatccacc acgcggacaa actcggtgat gcacagatcg tagtcgttaa
    18661 cttcggtcag caattcacgc accagagagt caagcacacc ctccatcgga gccagtaaca
    18721 cacgcatatc ttcacccgca aaaaaatgag gcgccatgtt agcgcctatg gtcaacggat
    18781 taaaggtctg caaagaggga ttgctgcaaa ctgtaatccc tctctatgct tgatgtatgc
    18841 ctgagttcgt tgcgcattgc gtaatgccgg ggagcgaaaa aataagcgct gatttcttaa
    18901 tgtgatcggt agcacgtttt acgaattaat tgtatgatga atccatctca tctggggtgt
    18961 tgattatgag taagacactg aacattatct ggcaatattt acgcgctttc gtcctgattt
    19021 atgcctgcct gtatgcaggc attttcattg cttccctgct accggtaacc attccgggca
    19081 gcatcatcgg tatgctgatc ctgtttgtcc tgctggcctt gcaaattctt ccggcaaaat
    19141 gggtcaatcc ggggtgctac gtactgattc gctatatggc gctattgttt gtgccgattg
    19201 gcgtaggcgt catgcaatat tttgatttgc tccgcgcaca gtttggcccg gtagtggttt
    19261 cctgtgcagt cagtacgctg gtggtttttc tggtggtgag ctggagttca caactggtac
    19321 acggtgaacg taaagtcgta ggtcagaaag gatcagaaga atgatggcga atatctggtg
    19381 gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg cacggtataa
    19441 gttccccctg cttaacccgt tgctggtagc aatggtggtg atcattcctt ttttaatgct
    19501 caccggcatc tcttacgaca gctactttaa aggtagcgaa gtgctaaacg atttgctgca
    19561 accggcggtt gtcgcgttgg cttatccttt atatgagcag ctacaccaga tccgcgcgcg
    19621 ctggaaatcg atcatcacca tctgttttat cggcagcgtg gttgcgatgg tgacggggac
    19681 ttccgtggca ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc
    19741 agtcaccacg cctattgcaa tggcggttgg cggcagtatt ggcggtattc cggcaatcag
    19801 cgcagtttgc gtgattttcg tcggcatcct cggcgcggta tttggccata cattgcttaa
    19861 tgcgatgcgt attcgtacca aagctgcgcg cggtctggca atggggactg cctcgcacgc
    19921 cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta gttcgctagc
    19981 gctggtgtta tgcgggataa ttacttcgct gatcgcaccg ttccttttcc cgattattct
    20041 ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt
    20101 gcataattaa tgagattcag atcacatata aagccataac gggttcgtaa actgttatcc
    20161 cattacatga ttatgaggca acgccatgca tccacgtttt caaaccgctt ttgcccaact
    20221 tgcggataac ttgcaatctg cactggaacc tattctggca gacaagtact tccccgcttt
    20281 gttgaccggg gagcaagtct catcgctgaa gagcgcaacg gggctggacg aagacgcgct
    20341 ggcattcgca ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa
    20401 tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca atatggaatt
    20461 tattggtgcg acaatgcagc aaaccgttca tgccgaacaa agcgcgatca gccacgcctg
    20521 gttgagtggt gaaaaagcgc ttgcagccat caccgttaac tacacgcctt gtggtcactg
    20581 tcgtcagttt atgaatgaac tgaacagcgg tctggatctg cgtattcatc tgccgggccg
    20641 cgaggcacac gcgctgcgtg actatctgcc ggatgccttt gggccgaaag atctggagat
    20701 taaaacgctg ctgatggacg agcaggatca cggctatgcg ctgactggtg atgcgctttc
    20761 tcaggcggcg attgcagcgg caaaccgttc gcacatgcct tacagtaagt cgccaagcgg
    20821 tgtcacgctg gaatgtaaag acggtcgtat tttcagcggc agctacgctg aaaacgccgc
    20881 attcaacccg actctgccac cgttgcaggg agcgttaatt ctattgaatc tcaagggtta
    20941 tgattacccg gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca
    21001 gtgggatgcc acctccgcaa cactgaaagc tctcggctgt cacagtatcg accgagtgct
    21061 tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg ccagccctct
    21121 cctggtgtcg aaattcccgg caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa
    21181 gtagcctgat ggaaattttc cttagatcga gtctcctgca tgttaaagcg cgtgttcctc
    21241 agcctgttag tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga tcgctggatg
    21301 agctggaaaa ccgcgcctta tatctacgac gaattgcagg atctccccta ccgccaggtc
    21361 ggtgtggtgc tcggaacagc aaaatattat cgtactggcg taattaatca gtattatcgc
    21421 taccgcattc aaggagcgat taatgcctat aacagcggta aggtaaatta tctattactg
    21481 agcggcgata acgcattgca aagttataat gagccgatga ccatgcgcaa agatttaatc
    21541 gctgctggtg tcgacccatc agatattgtt ctcgattacg caggctttcg tacgctggat
    21601 tccatcgtgc gtacacgcaa agttttcgat actaatgatt tcattattat cacccaacgt
    21661 ttccactgtg agcgggcatt atttattgcg ctgcatatgg ggattcaggc tcagtgttat
    21721 gccgtaccgt caccgaaaga tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc
    21781 ggtgcgctgg ctgaccttta tatttttaaa cgtgaaccgc gttttttagg gccgctggtc
    21841 cctattccgg ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc
    21901 gaacagttac ttgaattaca aaagaaacaa ggaaagtagt tatggatgta cagcagtttt
    21961 ttgtcgttgc cgtttttttc cttattccga tattttgttt ccgcgaagca tggaaaggct
    22021 ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc
    22081 gagcaaaaaa tcccggactc tttttcgctt atatggtggc atatatcggc ttcggaattt
    22141 tatctatcgg catgattgtt tatcttattt tctatcgtta atattttctc ttttctgtta
    22201 attattgaga ataattatta cttcactcaa taagctgcgg atataacatt ctgaaccgca
    22261 gctatttgtg aatcctttca cagtataaat ttccccacgc gcatagcctt aatatcagca
    22321 cgcaatattt atactctaaa taattcgagt tgcaggaagg cgacaagtga gtgagtcccc
    22381 aggagcttac ataagtaagt gactggggtg agcgaacgca gacgcagcac atgcaacttg
    22441 aagtatgacg agtatattta gcgttcgcct taattactca tagcattaag gaagatcaca
    22501 tgccgcaaca aaattatctg gatgaactca ctccggcttt tacgccttta ctggcgatta
    22561 aagaagcctc tcgctgttta ttatgtcatg acgctccctg tagtcaggct tgcccggcac
    22621 agaccgatcc ggggaaattt attcgctcaa tctactttcg taattttaaa ggcgctgccg
    22681 agacaattcg cgaaaataat gccctcggtg ccgtttgtgc cagagtgtgc ccgacggaga
    22741 aattatgtca aagcggttgt acccgtgccg gtgttgatac tcccattgat atcggtcgct
    22801 tacagcgttt tgttactgat tttgaacaac aaactgggat ggaaatttat cagcccggta
    22861 ctaaaatgct cggcaaagtc gcgattattg gcgcaggtcc tgccggattg caggccagtg
    22921 taacactgac aaaccagggt tatgacgtca cgatttatga gaaagaagcg caccccggtg
    22981 gctggttgcg taacggtatt ccgcaattcc ggttaccgca atcagtgctg gatgcagaga
    23041 tcgcccgtat tgaaaaaatg ggcgtgacca ttaagtgcaa caacgaagtc ggtaacacac
    23101 tcacccttga gcagctgaaa gcagaaaacc gcgcggtact ggtcaccgtg gggttatcga
    23161 gcggttccgg gttatcgctg tttgagcata gcaacgttga gattgccgtg gacttcctgc
    23221 aacgtgcacg acaggcgcaa ggcgatatca gcattccaca aagcgcatta attatcggcg
    23281 gcggtgatgt tgcgatggac gtagccagca cgctgaaagt tctcggctgt caggcggtaa
    23341 cctgcgtagc gcgtgaagag ttagatgagt ttccggcaag cgaaaaagag tttgccagcg
    23401 cccgggagct gggcgtttcg atcatcgatg gattcacgcc agtagccgtg gaaggcaata
    23461 aagtcacgtt taagcatgta cggttaccgg gcgaactgac gatggcggca gataaaatta
    23521 ttctcgccgt cggtcagcat gccagactgg atgcctttgc ggagttagag ccgcagcgta
    23581 acaccatcaa aacacaaaat tatcagaccc gcgatccgca agtctttgct gctggcgata
    23641 ttgttgaggg tgacaaaacc gtggtctatg ccgtgaaaac cgggaaagaa gccgccgagg
    23701 cgattcatca ctatttagag ggagcttgct catgttaacg aaagatcttt cgattacttt
    23761 ttgcggcgtg aagtttccca acccgttctg cctctcttct tcgcctgtag gcaactgcta
    23821 tgagatgtgt gccaaagcct acgacacagg ttggggcggc gtggtgttta aaacgatcgg
    23881 cttttttatc gccaacgaag tctcgccgcg ttttgatcat ctggtgaaag aagataccgg
    23941 ttttatcggc ttcaaaaata tggagcagat tgctgaacat ccgttggaag agaatctggc
    24001 cgccctgcgt cagctgaagg aagattaccc ggacaaagta ttgatcgctt cgatcatggg
    24061 ggaaaatgag cagcaatggg aggagctggc gcgcctggtg caagaagctg gcgcggatat
    24121 gatcgagtgt aacttctcct gtccgcaaat gacttctcat gcgatgggta gcgatgtcgg
    24181 gcaaagcccg gagctggtag aaaaatattg tcgggcagtg aaacggggtt ccacgctgcc
    24241 aatgctggcg aagatgacgc cgaatatcgg tgatatgtgc gaagtggcgc tggcggcgaa
    24301 gcgcggcggc gcagatggca ttgcggcgat taacaccgtt aaatccatca ccaatatcga
    24361 tcttaatcag aaaatcggta tgccgatcgt taacggaaaa tcgagtattt ccggatattc
    24421 cggtaaagcg gtaaaaccga tcgccctgcg cttcattcag caaatgcgca cccatccaga
    24481 actgcgcgat ttcccaatca gcggtatcgg cggcattgaa acctgggagg atgcggctga
    24541 gtttttattg ctcggcgcag caacgttaca ggtgaccacc ggcatcatgc agtacgggta
    24601 tcggatagtg gaagatatgg caagcgggtt gtcgcattat ctcgccgatc agggatttga
    24661 ttcgctgcag gagatggtag gtctggcgaa taacaatatt gtcccggcgg aagatttaga
    24721 ccgcagttat attgtctatc cccgtatcaa tcttgataaa tgtgttggct gtggacgctg
    24781 ttatatttcc tgttacgacg gcggtcacca ggcgatggaa tggagcgaga aaacccgcac
    24841 accgcattgt aataccgaga aatgtgtggg ttgtctgctt tgtggtcacg tctgcccggt
    24901 gggttgtatt gagctcgggg aagtgaagtt taagaaaggc gagaaagaac acccggtaac
    24961 gttgtaaaac cagagacgca tccggcattt ggcaccgatt gccggatgca gcgcatccgg
    25021 caatatccac aatcacatca cttcttacgc gcgtatttca gtgaatccag cgctacggcg
    25081 aagataataa tcgccccttt gatgatgtac tgccagtatg ggtttacgcc gatatacgtc
    25141 aggccatagt tgatgacggt aaaaataatt accccggtca ccacgccaat caccgtcccc
    25201 acaccgccgc tgaacgatac accgcctacc acgcacgccg cgatagcatc cagctcatac
    25261 ataaagccga ggttgttggt ggcagagccg atacgtccgg cttctaacat cccgccaaag
    25321 gcatagaaca cgccagacaa cgcgtagatc atcagcaggt tcaggccgac gttgacacca
    25381 gatacttttg ccgcttccgg gttaccgcca atggcaaaaa tgttcttacc gaagcgggtt
    25441 ttgttccaca acacccagac gaacgccacc gcaatcaacg cgtagaaggt gatgtaagag
    25501 agacggaaac tccccagcgc gacaaagccc tgagcaaagg tagagaagcc actgtcaaaa
    25561 ccagaaattg gcgacgcccc gacaaagtcg taatagagcg agttgatgcc atagacgatg
    25621 atcatcgtgc cgagcgtggt aatgaacggc gtcacgttga gataagcgat aatcagaccg
    25681 ttgatcaaac cgatcaccgc accaatggca cagacaatca gaataaccag cgcaatcggc
    25741 atcgtcgcca tttccgggaa cactttgttg gcgttatcca tggactgcaa taatgtcgca
    25801 gccaccactg ccgccagccc tacctgacga ccagcagaaa gatcggtccc ctgggtgaca
    25861 attaaccctg ccacaccgag cgcgataata atacgcaccg atgactgggt gagaatatta
    25921 cttaagttca acagacttaa aaatgttggg tcctggaaaa taataatcgc cagcaaaact
    25981 aaaagaacga cgtaaatacc gccctctttc aggtaagtaa gaaaactttt cttatttaac
    26041 gcactcatgg gaagccccta atcttaaagg tgcaaagacg caagacgcag aatttcgttt
    26101 tgcgttgttg tttttgtatc gacaattccg gaaacgagac cattgctcat gaccagaata
    26161 cggtctgtta tccctaacaa ctcaggcatt tcagaggaga taataataat ccccttgcct
    26221 ttcttcgcca gttcggcaat taactgataa atttcaaact tcgccccgac atcaataccg
    26281 cgcgtcggtt catcgagcat taatatttct ggttgcgtta gtagccagcg accaataatc
    26341 accttttgct gattaccacc ggagagcgaa ccaatttgcg tccgatgacc cggcgttttt
    26401 acccgcatcg aatcaatcac ccactgggta tcgcttttca tccgcgagtt atccagtaaa
    26461 ccaactttat ttttgtagtt gcgaatattg gaaattaacg agttaaaacc aatatccaga
    26521 taggcataaa ttcccgttga gcggcgctcc tcagttacca gtgcaaatcc gtggtttatg
    26581 gcttcgttgg cattatggtt attgatctgt ttgccgtgca acgtaatggt gccagccgat
    26641 ttctcgcgaa taccaaataa cgtctcaaca atatcggtac gtttcgcccc caccagcccg
    26701 gcaataccga ggatctcccc tttatgcaga tcaaacgaga catcgcgaat cgacggctgg
    26761 cgcagtgacg ttaggttacg cacctcgagg atgacttcgc ccggcttgtt ttctttgtca
    26821 ggaaaacgct gattaagaga acgcccaacc atcatggcga tgatcttgtc catcgtcagt
    26881 cctgccagcg gttcggtggc gatccactga ccgtcgcgca atacagtaac ttcatcacat
    26941 aactggaaga tttcttccat tttatgcgag atataaacaa taccgcagcc gcgctctttt
    27001 aatttacgaa taatagtgaa cagatgattg acctcttttt cggttaacga agaagtcggt
    27061 tcatccataa tcacaatttt cgcgttatag gaaaacgctt tggcgatttc gatcatctgc
    27121 atttgcgaaa cggataatgt gccgacgcgc gcacgcggat cgatatcaat atccagttca
    27181 tcaaaaatcg ctttggtttc gcggtacatt ttgtcctgat cgacaaacat gcctttggtg
    27241 ggatatcgcc ctagccacat gttatccatc accgaacgtt gtaataccag gtttaactcc
    27301 tggtgtacca tcgaaatacc attttccagt gcctctttgg cggaatggaa atcgatctct
    27361 ttaccctgga ataaaatggt gccggagtct ttttgataaa taccaaacag gcattttaat
    27421 aatgtcgatt ttcctgcacc gttttccccc attaatgcat ggatagaatg tggccggact
    27481 tttaaattaa cgttatcaag tgccttaaca ccaggaaagg acttgttgat accgctcatt
    27541 tccaacaagt attccccgga agacggagtc gttgagctga ccatataatt ttaccttgtt
    27601 ggccatacaa taagggcgca gtaaacgact gcgcccaatc agtcttattt cttgctgaat
    27661 tcagccaggt tgtctttatc tacgccaaca taaggtacgc ggaccacttt gttgtcgatt
    27721 ttccagttgg tgccatcagc cgcacctttg ccatcggcca ggtttttcgc cagatcaaag
    27781 gtcgctttcg cctggttgtt agcatcgttc agtactgtgc ccgccagtgc accggatttc
    27841 accagcgcca gcgcttctgg cagcgcatcg acgccaaaca ccggaatgct ggacttgttg
    27901 tgtgctttca gtgcttctac cgcgcccatt gccatcgcat cgttgttggc gataaccact
    27961 tcgattttgt tggcgttcgg gccagacagc caggcgtcca tcttatcttt cgcctgagcg
    28021 gtatcccaca tagcggtatc taactgtaac tgttcagttt taatgccttt gtcgttcagc
    28081 tctttaatca cgtaagtggt acgtgcttct gcatccggat ggcccggttc acctttcagc
    28141 aatacgaatt gaatctgacc gtctttgttc agatcccaac cctgattcgc cgcccagtgt
    28201 ttagcaatca aatcgccctg aataatgccg gactctttgg aatcagtgcc aacgtagtag
    28261 gctttgtcgt agctatccag cgccttacga gacggttctt tgttgaagaa aaccaccggc
    28321 acgttttgcc cacgcgcttt ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg
    28381 attgccagtg ccttcacccc tttcgccagc aatacgtcga tctgatcgtt ctgcttggac
    28441 tggtcattct gagaatcatt catcagcagc tgaacatctg gcgcggcttt cgcatcttgc
    28501 tcaatagcct tgcgcactac agacataaag ttatcgtcgt acttatagat tgttacacca
    28561 atgcgagtat cagcagcgtg tgcagcggca ccgaataaca tgctggccat cacagcagac
    28621 agggttaaca ccttcttatt catggtatct ccggtttttc ttatgcaggg tagtgcttga
    28681 gataaatgct cggcggggca gtagagttaa tgaagtgtta ctgaacgccg aagctcactt
    28741 tttaaaattc gttcttccat gcccggtaac gctccagaaa acggctttaa ttgttgttat
    28801 gacgctatta cctcggtgaa agtgattatt cactgttaca tacgggttac aacgttaaaa
    28861 cggtgcaatc atagctatca cattgttaag atactgtgaa atcactcaca gattgaaagc
    28921 ggttacatcg cctgatttgt tgagttagtg atcgccgccg cattctggcg cgttgcgaca
    28981 gaatgacgac gcactaacgt cggcataaaa cagtggctgg cacgaggatc aatattgcct
    29041 gctgccccct gcaaggccag ttcggtggct aatttagcca ttgaagcaat gggataacgc
    29101 acggtcgtta attgcgggtc ggtgtaacgg gcgatgggaa tatcatcgaa accgatgatt
    29161 gagagatgta acggaatcgc aatgccatta tcttttaatg ctgtcagtgc gccagcggcc
    29221 atattgtcgt tataagcaaa tacagcggta agttgtagat tgcgccccag cagttcaacc
    29281 atcgccgcct caccgcccgg catgtccggc gtaccagtgc caatccagct ttccggcgga
    29341 ataatatcct gctctttcaa cgcactcatc cagcctgctt tacgcatggc gtcatcttca
    29401 atgccgtggc tggaagaaag ataaccaata cgttgatgac cgttattcag caacatgcgc
    29461 gtcgccattc gggcaccgct gagattatcc aggcaaacgc aacgatgggc gtaccccggc
    29521 acaacgcggt tgattaacac cataccggga atgttatcca taaattgcgc cagttcatcg
    29581 tcactcaatg cttttgagtg aacaatcaac gcattacaac gctggcgaat taacacctca
    29641 atggcgtgac gctctttttc cgcttcatga tagctattgc cgattagcac gtatttctga
    29701 tgctgctggg cgaccagatc caccgctttt accagcgcgc cgaaaaaagc atcagaaacg
    29761 tccatcacca ccacgccaat ggtatcgcta acctgagttg ccagcgcctg ggcattggca
    29821 tttggccgat aatccagctc actcacggct ttcattactg cttcacgcgt gtcggcactg
    29881 accagcgtgc tgttattgag cacccgggaa accgttgcca cagagacgcc agcctgacgc
    29941 gctacatcac gaatggtgat catattcact acctgttatg agattgcagt aaatgactgc
    30001 ttgctggcgg ctattttgtc agcactgaat acaggacttc gtgaatcgag tcacagcaat
    30061 ggaaacggtt acagccgttt tgttaacgaa tgtgatccag atcgttatct tgatgtttta
    30121 gatattgccg ttccggcagc acgcaaagtt aactgacgcc agagccattc caccggcccc
    30181 tggcggaagt aacgcagcca gataacagag aagaggatat tcgccagcca taccggaata
    30241 acaaacgcca gcagctccag gcgatcaaaa tgcataaaca aaccgaggtg gtaaaaaagc
    30301 atggtacaaa tcagcgtttg caatagatag ttggtcagcg ccatccgtcc gacgcaggcg
    30361 atcgcaagca ccagcttaaa gcggctcaat tgcggccaga agccataaaa cagcgacgca
    30421 tagccaatcg cctgaaacgg cgcactcagt tcccgcggca tttgaagtaa gaaggcacac
    30481 cagcgatacg cccagtccag ctgccattgc agggcgatgg caggaaggtt aatggtcacc
    30541 ccaatcgcca ccagcacaaa accagtacga cgatagtgac gtaagctgaa ctgccctttc
    30601 agccagccgc tgcgcatcag cgccgcacca atgagcatca tccctgccag ttgccagcca
    30661 tactgtgcgc ccagtgccag taaactgttg ccaacaccat cggcacgatt actgatcgct
    30721 tcaacgccgc cgtgaagctt ccagtatttt tcatacaaaa tagccgatgc atccggcgtc
    30781 caggcgcggc tggtctggct gtcggaaatc agccccaata acagcaaaac gccaagcccc
    30841 accagataaa gcatgacgcc tgtattaaac aggcttttta ccgatggcgc atcgcgcacc
    30901 agccgccagc agattaagcc caccagcccg taagccagca gaatatcgcc gtcccagaac
    30961 aataaaccgt gaataaaacc cagcaagact aacagcgtta accgcgactg gatccagcgt
    31021 ctgccacggg gcagcaacat ttgcaggccc gcaccaaaca gcagcgcaaa aagcgtgagg
    31081 aatttcacct ggccgacgag atcaagaaat gcccaggtcc atgcatcctg cggcgtaata
    31141 gcgccgtacc aggcgggatt gagataagcc gcctttggta gcccaaaggc gctgatgttt
    31201 aatagcagga tccccagaat ggcgacgccg cgaacaaaat cgagcgtgac gttgcgctcc
    31261 atggttcctg ccttttaatc agttgtgatg acgcacagcg cgcagaaact cgtggcgcgt
    31321 attctgactg gatttgaaca atccaccaag agaggtcgtt gtcgtggcac tggttgcatc
    31381 gcggatgcca cgcgccttca cgcagtaatg caccgcgtcg atcgagacag ccacgttatt
    31441 ggtgcccagc agcgtttgta gcgcaataag aatttgctgc gtcagacgtt cctgcacctg
    31501 cggacgctgg gcaaagaact gcacgatacg gttaattttt gacagaccga tcaccgaatc
    31561 tttcgggata taagccaccg tcgctttgcc atcgatggta acaaaatggt gttcacaggt
    31621 actggtcaga gtgatatcgc gcacggtgac catttcatcg accttcattt tgttttcaat
    31681 gagggtgatt ttcgggaagt tggcgtaatc cagaccggag aaaatttcat cgacatacat
    31741 tttagcgatg cgatgcggcg tttccatcaa actgtcatca gccaggtcga gattcagcag
    31801 ctgcatgatt tcggtcatat gaccagcaat aaggcttttg cgcgtttcgt tatccatttc
    31861 atgcacgggc gggcgcagcg gtgtttccag tcctcgcgca actaacgctt catgaaccag
    31921 ggccgcttct ttactgagtg atggcattta tgatttctcc tgcaggtgtg acgcctccgc
    31981 cctgcgtggg ggcaaagtta ttaagctgat ttacagcctg attattgtgc gtgaggcggc
    32041 gcacataatc cagtattcac agcgataatt attgtaattg ccgctgcctt tcatcagcag
    32101 atgttaaaac atcgttatgc aaatacggaa gtgaaagtta ctcacagcac attgaataaa
    32161 cggtatgatg aagaaattgc aaacaacaca acaaggagcc acgcatggaa atgctcgaag
    32221 agcaccgctg ttttgaaggc tggcagcaac gctggcgaca cgactccagt accttaaact
    32281 gcccgatgac gttcagtatc tttctcccac cacctcgtga tcacacccca ccaccagtgc
    32341 tgtactggct ttccggatta acctgcaatg acgagaactt caccaccaag gcgggtgccc
    32401 agcgggtagc ggcggaactg gggattgtac tggtgatgcc agacaccagc ccgcgcggcg
    32461 aacaggttgc caacgacgat ggctacgatt taggccaggg cgcaggcttt tatcttaatg
    32521 ccacgcaacc gccgtgggcg acgcattacc ggatgtatga ttatctgcgc gatgaattac
    32581 cggcgctggt tcagtcgcaa tttaatgtca gcgaccgctg cgccattagc ggtcattcaa
    32641 tgggtggtca cggtgcgctg attatggcgc tgaaaaaccc gggcaaatac accagcgttt
    32701 ccgcctttgc gcccattgtg aatccgtgca gcgtcccgtg ggggatcaaa gcgtttagcc
    32761 gctatttagg tgaggacaaa aatgcatggc tggaatggga cagttgcgcg ctgatgtatg
    32821 ccagtaacgc gcaggatgcg atcccgacgc ttatcgatca aggtgataat gatcagtttc
    32881 ttgccgatca gttgcaaccc gcagttctgg cagaagccgc gcgccagaaa gcgtggccga
    32941 tgacgctgcg tattcagccg ggatatgatc acagttacta cttcatcgcc tcttttatag
    33001 aggatcacct gcgcttccat gcgcagtatt tactgaagtg aaagtccgcc cggttcgccg
    33061 ggcatcttct catcagaagc gataatccac tgccataaag taacgacgtc cgtcttcgtt
    33121 atagctgtag tcatcacggc tgagatcctt gtcgccaagg ttcagcacgc ctgcgcgcag
    33181 tttgacgtct ttagtcacct gccaggccgc gccggtattc cagatggtat aaccgccagg
    33241 tgttttagcc gtcgcgctgt cggctcgttt ctgcccggta tagtgaccag aaacatagaa
    33301 tgaccagtct tccagcgcca gcggtttcca gtccagcgta ccgttagcgg tatggaacgg
    33361 cagatcggat agcggtttgt tttcgccgtt gctgacatca cgaccatcgt tgtaggtgta
    33421 gttgaatgac agtttccatt catcgttgaa cggaattttc agttcggttt ccacgccctg
    33481 aatacgagct ttgttaacgt tgtagtagct aaataccggt atgcgccgtc cgttagcgcc
    33541 cgtctcaaaa ccgacaaagt tttggtagcc cggtgcagcg ttgacgtcag acgtacggct
    33601 gatgctgata cgatctttca catcgttacg gaaaacggta acgctggatt caaccccttc
    33661 cagccagcct tcttcaccca tgtagtaaag ccccagctcc caactttcgc tggtttctgg
    33721 tttcagatcc gggctaccca caatcttaca tgcgccacgg caggaattgc tcgtccagtc
    33781 agggctaagt tgcaacagag aaggtgcttt aaatgccgtc gcccagcccc ctttcaccgt
    33841 tacggtgtcg gtggcgttat aaaccaggta ggcacgcgga ctccagtgtt caccgtaggt
    33901 ttcgtgatcg tccatacgca cgccggtcgt cagcgccagc ggctcgaaga tccgccattc
    33961 atcttccaca aacagcgcgt actggctggc agacgttttg gagctggttc ccccggtcag
    34021 gttcaccgca tcgctaagtt tgtcgtgacg ccattcaccg ccaaccgtga gaaactgatt
    34081 aatcgccgtc agcggcaacg tgtatttgcc gtcgaccgta ttgctttcgg aagttatcgg
    34141 gctgctgttg ccagggtttt tgttctcgac tttctcaccg tagtatttca gttcgctggt
    34201 gccgtaatcc caacgcccat tatggctgac ggagtagttc tggcgttcca ggcggttttt
    34261 gtccagcgag tcggaatcac gatcctgacg gtcgaaaccg tatccggcag taaaatcgtg
    34321 attttgattc ggtgtccagg caaattcgac attgccgtcg cggctggaga atccttcaat
    34381 acgcggcgtt tctccggtat cggtggtcgt tgagttttgc gggtcatcct tttcacgttt
    34441 tgctaggctg ccgtaagctt tcattcccag cacaccatca attaatggtc cactggtaaa
    34501 gaactgaccg ttataggtat caccgcgatc gcgatgttcc tgaatggtgg tatcgacggt
    34561 aacggtaccc gaccatttct gaccgatttt tttggtgatg atattcacta caccgccgag
    34621 cgcatcggaa ccgtacagcg acgacatcgg gccacggacc acttcaatac gttcgatgga
    34681 atcgaccggg atccagttca gatcgaaatc attgtggcgg aagacggcat tgcgggagtt
    34741 cacgcgttta ccgtcgacga gaatcagggt atagctgctg tccagaccac gaatactaac
    34801 gcccttacgg ttatcccctt cgttcgtcag ttgtacgcca ggcacttctt tgaggacatc
    34861 cttcagattc tgtaccggtt ttcgctgcag gtcttcctgg gtaatgacgc tgatactggc
    34921 aggtgcatct ttaagatttt gttccacgga agatgcagtg acaaccatcg tttcgccatc
    34981 atcatcgacc gctaacacag gccatgcaca agaaatagcg gacaaacaca gcccgacccg
    35041 tacgaaaggg ttcaacctaa acattccata tctccatgag gtaactacga aaataaaatg
    35101 ggttatcgct cacatcttct tcacgtcccc ttgcgtacgg cagcatcgcg gtggacttat
    35161 tttttatgca ggtgatcatc ccagaaagcc ggaacagcct ctgatttgtc agcctttttt
    35221 gattgcggcg taacgataat gcaaacgata acaattatca atccaaatgt taaattttat
    35281 atccgcatga tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag
    35341 caaggaaggg aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtaacgt
    35401 acgaagtgag ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata
    35461 ccaatatacg ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag
    35521 ttctggccca aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag
    35581 aaacctgaac ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg
    35641 aagcgatagt ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg
    35701 ttcagcagcc acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacagaccg
    35761 gcaatcaccg tcgtcgcata cagcgcatta cgcggcacgc caccacgcga cagtttagcg
    35821 aaaatgcgcg gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac
    35881 ataccggagt tacctgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc
    35941 gcagagagca gacccgcgtg ctggaacacc agggtgaacg gactaacgct gatgtcttta
    36001 acatcgttac gcagcaggct cggatcggtg tacggaataa tcaggctgat aatcaggatc
    36061 gcgaacacat agaacaacag gattcgccag aacacctgac gtaccgcgcg tggaatgttt
    36121 ttcgccggat cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag
    36181 aagccgacaa tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg
    36241 ccgattgtcc agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc
    36301 agcacgccaa cgatgataaa gacaataact gtcgtgactt tgatcagtga gaaccagtat
    36361 tccgcttcac caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac
    36421 aacgcactcc agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgagct
    36481 gcaaccaggt caacggcgat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg
    36541 aagccaaagc cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc
    36601 atatatgcag ccagttcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc
    36661 atatacgaga gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca
    36721 aaaagacctg taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat
    36781 tcacggcgta agcccggcgc ttctgtggtt ttagtttcgg aaaccataca aaaatgctat
    36841 ccatcttaaa aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac
    36901 acggttataa gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt
    36961 tctggcgaat gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct
    37021 tgtacagcac cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc
    37081 gcgttggaaa ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggc
    37141 agagggaccg caacttcact taatgtgcct gcctgcaatt gatcttcaat cacccgtcgc
    37201 gacaggcaac taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct
    37261 aattccatcg ccatctcaaa cttcggtaaa tgtgacagca atagataatc gacaatctcc
    37321 cgcgtgccgg aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg
    37381 accggaccac gagccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc
    37441 cacggttcag aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg
    37501 cggaaatcca gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc
    37561 aactgcggat aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg
    37621 gtactactgg catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca
    37681 accgcctgtt caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca
    37741 accagtcttt tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc
    37801 aaggctgcgc tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat
    37861 ccacttttca atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct
    37921 taccacttat aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc
    37981 gtaaccttat gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca
    38041 tcgtacactg tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc
    38101 cctgtggggt ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc
    38161 aatcttgttg gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga
    38221 cggtggtgtg ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt
    38281 ccgtctgacg ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt
    38341 gacgctgtcc agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga
    38401 taagcacacc agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact
    38461 ggcgactgag ccggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt
    38521 tgttatcttc gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca
    38581 atggtttagt ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca
    38641 ggtggtggcg gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa
    38701 aatgctgcgc gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca
    38761 gctgtctggg gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt
    38821 gttcatcgtc gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat
    38881 gctggtaacg ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac
    38941 tcacgtcagc gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt
    39001 tgcctggctg attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata
    39061 aaccactaca tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta
    39121 acaggagtcc tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa
    39181 atgccgcaat tcgcgccgcc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc
    39241 aacgtcagtg gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct
    39301 gtgaaaaata tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc
    39361 tcggacatcc ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc
    39421 agcgttgcga acaactgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc
    39481 agatttcaga agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata
    39541 aaactcaagg tgtaacagcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt
    39601 ttaaattcga acatctcgcg gcgattatcg acggcgtgga agataaatcc cgcgtcggcg
    39661 tctgcattga tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat
    39721 gcgagaaaac gttcgcggat tttgcccgta ttgttggctt taagtatctg cgcgggatgc
    39781 accttaacga tgcgaaaagc acctttggca gccgcgttga ccgccatcat agcctcggtg
    39841 aaggcaatat cggtcatgat gcgttccgct ggatcatgca ggacgaccgt ttcgacggca
    39901 ttccgctgat ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga
    39961 aagcgcaaca aactgaaaaa gcggtagcct gaagatgaat aaccgggaaa aggagatcct
    40021 tgcaatttta cggcgtaacc cgctgattca gcagaacgaa attgcggaca tgctgcaaat
    40081 cagtcgttcg cgcgttgcgg cgcatattat ggatttaatg cgcaaagggc ggattaaagg
    40141 caaaggttac attctcaccg agcaggaata ctgcgtagtg gtggggacaa tcaatatgga
    40201 tattcgcggg atggcggata tccgttaccc gcaagcggct tctcatcccg gtacaattca
    40261 ttgctcggcg ggcggcgtgg gacgcaacat cgcccacaat ctggcgctgt taggccgtga
    40321 cgtccatttg ctttcagtga ttggcgatga cttttatggc gaaatgctcc tggaagaaac
    40381 gcgccgcgcc ggcgtgaatg tctccggctg cgttcgttta catggtcaaa gcacatcgac
    40441 gtatctggca attgccaatc gagacgatga aaccgtgctg gcgattaacg atacccatct
    40501 gctggaacag ttatcgccgc aattattgaa cgggtcgcgc gatttacttc gtcatgcggg
    40561 cgtggtactg gctgattgca acctgacagc cgaggcgctg gaatgggtct ttaccctcgc
    40621 tggtgagatc ccggtgtttg tcgataccgt ttcagaattc aaagcgggca aaatcaaaca
    40681 ctggctggcg catattcaca ccctgaaacc cactttaccg gagctggaaa ttttatgggg
    40741 acaggcgatc accagcgatg ctgaccgtaa tgctgcagtg aatgcgttgc atcagcaagg
    40801 tgttcagcaa ctgtttgttt atttgcccga tgagtcagtt tattgcagcg aaaaggatgg
    40861 agaacaattt ttgctgaccg caccagcgca tacgacagta gacagttttg gtgctgacga
    40921 tggttttatg gcgggcctgg tatatagctt tctggaagga aacaatttcc gtgacagcgc
    40981 ccgttttgcg atggcctgcg cggcaatttc acgcgccagc ggcagcttaa acaaccctac
    41041 cctgtctgcc gataacgcgc tttcattagt gccaatggtg taacaatgtt gccggattcg
    41101 acgcttgacc gcatcttatc cggctcataa atcgcacact acgctaaacc aataaagaac
    41161 ccggcaatgg tggcactcat caggttagaa agtgtcgccg ccgccagcgc gcgtaaacca
    41221 agctgggcga tttccggcgc acggtgtggc gcaaccgcag aaaacgcccc caccaccacc
    41281 ccgatagaac caaagttagc gaaaccgcac aacgcgaagg aaataatcgc cacggtttta
    41341 gcatccagag tgccagccgt ttgcagatag ggtgagaaat tgagataagc gacaaattca
    41401 tttattgcca gtttctgtcc aatcaaactc ccggcaagat tcgcatcagt ccagtcaaca
    41461 cccattaccc acgccagtgg ggccagcagg taacctaaaa tggactccag cgaggcatgt
    41521 tcaaaaccaa accagccgcc aacgccgccg ataataccgt taattaacgc aatgatggca
    41581 acaaatgcca tcaccactgt tgccacaccc gcagcgattt tcagcccggt catcgcccct
    41641 gtcgcggcgg cttcaataat gctttttggc ggtgtttcgg tgaaagagag gttattaaaa
    41701 gaaacctgcg aagattccgt agccgggctt aacaggcggg caaacaagat cccgcctggg
    41761 atcgccatta acgatgccgc cagcaaatat tccacaggta cgcccagtgc ggcgtaacca
    41821 atcattgtcg aaccggcaat tgaggccatg ccactacaaa tcgctgtaaa taattcattg
    41881 cgattcagcc gatcgataaa gggtttgacg attgccggaa tttcgttttg cccgaggaaa
    41941 atggtagtga ccgcgacgaa tgactcgatc ttgctgatat ttaatgcttt ctggaagata
    42001 ccgccgagaa tgcgaattaa gatccccatc acaccgatgt aatagagaat actcaccagc
    42061 gcggtgacga agataattgc cggtaatact ctgaaaccaa agataaatcc tgcgccatca
    42121 aacagcgtgt ccattttcgg cccgaccagc gaaccgaaga taaatgcgct acccgcgtcg
    42181 ctgtacgcca tcactttatg cacgccaaaa gcgacttttt cagcgaccca acgccctggc
    42241 ggtaaccaaa gcataatgcc gccaatcaca acctgtaaca ctaacgccgc gccaacggta
    42301 cgcaggctga tcttcttctt gtttactgac agcaaaaatg caattgccag caataccacc
    42361 attcccagaa cacttctcat gacatccata gtgattatct cttcatgtca tattgggcga
    42421 cggctttctg cccctcagaa atagccaggc ctgcatcttg cgataccggt ccggcttgtt
    42481 atcaacactt aatgggtttt gatgtagccg cgcacgcact cttcgactaa gccccagaac
    42541 cagtcggtat caatagtgat gccgactttg gtattagcgg gcttgccaag aacgcccagc
    42601 tcgtcgcaga cggtacgccc ataacaaggg ccactgttga cgtccacttc gacgtacatc
    42661 tcctgggttt taatgccatc agggttaatc agataaccga tgcaggtggc gtcgtgcacc
    42721 gggccgccag ccaggccgta gttttcgaac tgcgttttga gagtgaagtt catgatgtcg
    42781 ctgaacagct ctccggcagg gccgcctgcc ctttccatcc gagcaattac gtccggggtg
    42841 caaacggtct ggttggtaag atcgaggccc atcatcacta atggaacgcc agaggtgaac
    42901 accacgcgtg cggcttccgg gtcagcaaag atgttgaact cggcagatgg cgtgaagttg
    42961 cctgtaccgt aagcgccgcc cattagcaca atttcacgga ttttgggcag gatcgcgggt
    43021 tgcatacgca ttgccaccgc gatatttgaa agcggaccaa ccggcaccag agtgatatcg
    43081 ccatcgcttg ccatcagggt atcaatgatg tatttcaccg cgtgggtact ttctgcctgg
    43141 cgggtcagcg gctcgaatac cgggccatcc agtccggttt caccgtgaat attatcggca
    43201 acgatttgtt gacgcataat gggctgcggc atccccgcat aaaccggaac attaatctcc
    43261 agtttctggc aaacattcag gccattaatt aatgttttat caagcgtctg attacccgct
    43321 acaatagtga tgcctaataa atctattgcc ggatgtttcg ctgccatcat cattgcaata
    43381 gcatcatcat gacccggatc acaatccaga ataatttttc tcttttccat tgtttatttc
    43441 ctctgtttcc agttgcgtta ttttttctac agcaaagaaa attcgaccgg gccgatgatt
    43501 gaatcttaac aacagcgtac gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg
    43561 tgaaagaaat ccacaataat gatcttaagc tgcaattgat gagtgaatcc gcgtttaagg
    43621 attgcttttt aacggatgtt tcagccgata cgcggctgtt tcatttttta gcgcgtgact
    43681 acattgtgca ggaagggcaa cagccgtcct ggctgtttta cctgacgcga ggccgcgcca
    43741 ggctttacgc cacgctagct aatggtcgcg tgtcgctgat cgatttcttt gccgccccct
    43801 gttttattgg cgagattgag ttaatcgata aagaccatga accacgtgca gtacaggcga
    43861 ttgaagagtg ttggtgcctt gcgctcccta tgaaacatta ccgtccgctg ttattaaacg
    43921 acacgctatt tttacgaaaa ctttgcgtca ccttaagtca taaaaattat cgtaatattg
    43981 tttctttaac tcagaatcaa tcatttccgt tagtgaatcg cctggcagca tttatattac
    44041 tctcgcaaga aggtgatctt tatcacgaaa agcatacgca agcggcagag tatttaggcg
    44101 tttcttatcg acatctttta tatgttctcg cgcaat
//
LOCUS       DAPHJE010000026        43269 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPHJE010000026 DAPHJE010000000
VERSION     DAPHJE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.026"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..640
                     /locus_tag="RH222_001432"
     CDS             56..640
                     /locus_tag="RH222_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3796482.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            720..1415
                     /locus_tag="RH222_001433"
     CDS             720..1415
                     /locus_tag="RH222_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3796483.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDNVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1445..3961
                     /locus_tag="RH222_001434"
     CDS             1445..3961
                     /locus_tag="RH222_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3796484.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYSGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3972..5063
                     /locus_tag="RH222_001435"
     CDS             3972..5063
                     /locus_tag="RH222_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3796485.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLKANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5106..5966)
                     /gene="rsmI"
                     /locus_tag="RH222_001436"
     CDS             complement(5106..5966)
                     /gene="rsmI"
                     /locus_tag="RH222_001436"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV3796486.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6031..8067
                     /gene="lpoA"
                     /locus_tag="RH222_001437"
     CDS             6031..8067
                     /gene="lpoA"
                     /locus_tag="RH222_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV3796487.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8025..8420
                     /locus_tag="RH222_001438"
     CDS             8025..8420
                     /locus_tag="RH222_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV3796488.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8440..9030
                     /gene="diaA"
                     /locus_tag="RH222_001439"
     CDS             8440..9030
                     /gene="diaA"
                     /locus_tag="RH222_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV3796489.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9040..9615
                     /gene="yraP"
                     /locus_tag="RH222_001440"
     CDS             9040..9615
                     /gene="yraP"
                     /locus_tag="RH222_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV3796490.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9820..10860)
                     /locus_tag="RH222_001441"
     CDS             complement(9820..10860)
                     /locus_tag="RH222_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV3796491.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10933..11568)
                     /locus_tag="RH222_001442"
     CDS             complement(10933..11568)
                     /locus_tag="RH222_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV3796492.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11696..12214
                     /gene="yhbO"
                     /locus_tag="RH222_001443"
     CDS             11696..12214
                     /gene="yhbO"
                     /locus_tag="RH222_001443"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV3796493.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12194..12637)
                     /locus_tag="RH222_001444"
     CDS             complement(12194..12637)
                     /locus_tag="RH222_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV3796494.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12688..12990
                     /gene="yhbQ"
                     /locus_tag="RH222_001445"
     CDS             12688..12990
                     /gene="yhbQ"
                     /locus_tag="RH222_001445"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV3796495.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12977..13480)
                     /locus_tag="RH222_001446"
     CDS             complement(12977..13480)
                     /locus_tag="RH222_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3796496.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13474..13998)
                     /locus_tag="RH222_001447"
     CDS             complement(13474..13998)
                     /locus_tag="RH222_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV3796497.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14207..15202
                     /locus_tag="RH222_001448"
     CDS             14207..15202
                     /locus_tag="RH222_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV3796498.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15211..16089
                     /locus_tag="RH222_001449"
     CDS             15211..16089
                     /locus_tag="RH222_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV3796499.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16295..17302
                     /locus_tag="RH222_001450"
     CDS             16295..17302
                     /locus_tag="RH222_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV3796500.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17420..18664)
                     /gene="mtr"
                     /locus_tag="RH222_001451"
     CDS             complement(17420..18664)
                     /gene="mtr"
                     /locus_tag="RH222_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV3796501.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18818..20707)
                     /gene="deaD"
                     /locus_tag="RH222_001452"
     CDS             complement(18818..20707)
                     /gene="deaD"
                     /locus_tag="RH222_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV3796502.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20700..20780)
                     /gene="yrbN"
                     /locus_tag="RH222_001453"
     CDS             complement(20700..20780)
                     /gene="yrbN"
                     /locus_tag="RH222_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV3796503.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20887..21771)
                     /gene="nlpI"
                     /locus_tag="RH222_001454"
     CDS             complement(20887..21771)
                     /gene="nlpI"
                     /locus_tag="RH222_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV3796504.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21880..24015)
                     /gene="pnp"
                     /locus_tag="RH222_001455"
     CDS             complement(21880..24015)
                     /gene="pnp"
                     /locus_tag="RH222_001455"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV3796505.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24262..24531)
                     /gene="rpsO"
                     /locus_tag="RH222_001456"
     CDS             complement(24262..24531)
                     /gene="rpsO"
                     /locus_tag="RH222_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV3796506.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24680..25624)
                     /gene="truB"
                     /locus_tag="RH222_001457"
     CDS             complement(24680..25624)
                     /gene="truB"
                     /locus_tag="RH222_001457"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV3796507.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25624..26025)
                     /gene="rbfA"
                     /locus_tag="RH222_001458"
     CDS             complement(25624..26025)
                     /gene="rbfA"
                     /locus_tag="RH222_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV3796508.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26189..28861)
                     /gene="infB"
                     /locus_tag="RH222_001459"
     CDS             complement(26189..28861)
                     /gene="infB"
                     /locus_tag="RH222_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV3796509.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28886..30373)
                     /gene="nusA"
                     /locus_tag="RH222_001460"
     CDS             complement(28886..30373)
                     /gene="nusA"
                     /locus_tag="RH222_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV3796510.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30401..30853)
                     /gene="rimP"
                     /locus_tag="RH222_001461"
     CDS             complement(30401..30853)
                     /gene="rimP"
                     /locus_tag="RH222_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV3796511.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(31060..31136)
                     /locus_tag="RH222_001462"
     tRNA            complement(31060..31136)
                     /locus_tag="RH222_001462"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31100..31102),aa:Met,seq:cat)
     gene            31484..32827
                     /gene="argG"
                     /locus_tag="RH222_001463"
     CDS             31484..32827
                     /gene="argG"
                     /locus_tag="RH222_001463"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV3796512.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32835..34460)
                     /locus_tag="RH222_001464"
     CDS             complement(32835..34460)
                     /locus_tag="RH222_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3796513.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVILFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34919..35005)
                     /locus_tag="RH222_001465"
     tRNA            complement(34919..35005)
                     /locus_tag="RH222_001465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34969..34971),aa:Leu,seq:gag)
     gene            complement(35020..35352)
                     /gene="secG"
                     /locus_tag="RH222_001466"
     CDS             complement(35020..35352)
                     /gene="secG"
                     /locus_tag="RH222_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV3796514.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35580..36917)
                     /gene="glmM"
                     /locus_tag="RH222_001467"
     CDS             complement(35580..36917)
                     /gene="glmM"
                     /locus_tag="RH222_001467"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV3796515.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36922..37758)
                     /gene="folP"
                     /locus_tag="RH222_001468"
     CDS             complement(36922..37758)
                     /gene="folP"
                     /locus_tag="RH222_001468"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV3796516.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     EN"
     gene            complement(37848..39782)
                     /gene="ftsH"
                     /locus_tag="RH222_001469"
     CDS             complement(37848..39782)
                     /gene="ftsH"
                     /locus_tag="RH222_001469"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV3796517.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39882..40511)
                     /gene="rlmE"
                     /locus_tag="RH222_001470"
     CDS             complement(39882..40511)
                     /gene="rlmE"
                     /locus_tag="RH222_001470"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV3796518.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40637..40930
                     /gene="yhbY"
                     /locus_tag="RH222_001471"
     CDS             40637..40930
                     /gene="yhbY"
                     /locus_tag="RH222_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV3796519.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(41086..41562)
                     /gene="greA"
                     /locus_tag="RH222_001472"
     CDS             complement(41086..41562)
                     /gene="greA"
                     /locus_tag="RH222_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV3796520.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41810..43243
                     /gene="dacB"
                     /locus_tag="RH222_001473"
     CDS             41810..43243
                     /gene="dacB"
                     /locus_tag="RH222_001473"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV3796521.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
BASE COUNT        10688 a        11623 c        10604 g        10354 t
ORIGIN      
        1 aacatatatt cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa
       61 taaagttaca aaaacagcta ttgcgggctt acttgcgctt ttcgcaggta atgccgctgc
      121 aaccgatggt gaaatagttt ttgatggcga gattttaaaa tccgcttgtg aaatcaatga
      181 ctctgataag aaaattgaag ttgctcttgg tcactataat gccgaacagt ttcgttctgt
      241 aggcgatcgt agcccaaaaa ttccattcac tattccttta gttaactgcc cagtgactgg
      301 gtgggagcac gataacggca acgttgaagc gtctttccgt ttatggctgg aaacgcggga
      361 taatggtacc gtccctaact tccctaactt ggcaaaagtg ggctcttttg ctggcacagc
      421 agcaacaggt gtgggtatcc gtattgacga tgcggaaagc ggaaacctta tgccactaaa
      481 tgctatgggc aatgataaca cagtttatca gatccctgcg gactccgctg gaattgtcaa
      541 tgttgacctc atcgcttact acgtatcaac cgttgaagca tcagaaatca ccccagggga
      601 agcagacgct gtcgttaacg taacgctgga ttaccgttaa tacgttacgg cgttatctga
      661 cctttcagat aacgcccttt tccttcctct ttctcgttgt atcaggttga aaaataacta
      721 tgtcaaaacg aacattcgcg gtgataataa ccttgttgtg tagcttctgt atcggccagg
      781 cgcttgcagg gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag
      841 aggctgcgtt acctgtcgca aacaaaggcg cagaaacgcc ttatttactg caatcatggg
      901 tagataatat agatggtaca agccgtgccc catttattat aaccccaccg ctatttcgtc
      961 ttgaagctgg cgatgattca tcactgcgaa ttattaaaac agctgataac ctgcctgaaa
     1021 ataaagagtc gctgttctac attaatgttc gtgccattcc agcaaagaaa aaatcagata
     1081 atgttaatgc taacgagttg acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc
     1141 ccgcacacct gaagggacgg gtaaacgatg cgtggaaatc actggaattt aaacgtagtg
     1201 accattcact caatatatat aacccaactg aatattacgt cgtatttgcc ggactggcag
     1261 tcgataaaac cgatctcaca agcaaaattg aatatatcgc acccggagaa cataaacagt
     1321 taccacttcc tgcatctggc ggaaagaacg tgaaatgggc tgcgatcaat gattatggcg
     1381 gcagttccgg gacagaaact cgtccactgc aataacaaat ataaaaaaca caggtcatca
     1441 gggaatgcca caacgacacc accagggaca taaacgcaca ccgaaacagt tggcgctcat
     1501 tatcaaacgc tgtttgccga tggtgctcac tggcagcggc atgctttgca ctaccgctaa
     1561 cgccgaagag tattatttcg accccattat gctggaaacc acaaaaagtg gtatgcaaac
     1621 aaccgatctg tcacgatttt caaagaaata cgcacaacta ccgggaactt atcaggttga
     1681 tatctggctg aataaaaaga aggtttcaca gaaaaaaatt acatttaccg ccaatgccga
     1741 gcaacttctg cagccacagt ttacggttga acaactacgt gagctgggta ttaaggtgga
     1801 tgaaatcccg gcgctggctg aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat
     1861 cattcccggt acagctgctg aatttgattt caatcatcag cgacttaatt tgagcattcc
     1921 ccaaattgca ctgtaccgtg atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg
     1981 tataccaacg ctgtttacca actactcgtt tacaggttct gataaccgtt accgccaggg
     2041 caatcgtagc caacgacagt acctgaatat gcaaaatggt gctaattttg gcccctggcg
     2101 attacgcaac tattccacat ggacacgcaa cgatcagacg tcaagctgga acactatcag
     2161 tagttattta caacgtgata tcaaggcgtt gaaatctcag ttgcttctgg gagaaagcgc
     2221 caccagcggc agtatttttt ccagctacac ctttactggc gtgcaactcg cttccgacga
     2281 taatatgttg ccaaacagcc agcgcggatt tgccccaacg gtacgcggta tcgcaaacag
     2341 tagtgcaatc gtgactatca ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc
     2401 gggtgccttt gaaattaacg atctctaccc ctcttccaac agcggcgatt tagaagtcac
     2461 gattgaagaa agtgacggta cgcaacgtcg ctttatccag ccttattctt cattacccat
     2521 gatgcagcga cctgggcatc taaaatatag cgcgaccgct ggacgctatc gcgctgatgc
     2581 aaacagtgat agcaaggaac ccgaatttgc tgaagccacg gcaatatatg gtttgaataa
     2641 tacttttacg ctgtatagtg gcctgctcgg ttctgaagat tattatgcgc tggggatcgg
     2701 tatcggcggc acacttggcg cactgggcgc gttgtcgatg gatatcaaca gagctgacac
     2761 ccaattcgat aaccagcact cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga
     2821 tatcccggaa accaacacca atatcgctgt cagctactat cgctatacca acgatggcta
     2881 ttttagtttt gatgaagcca atacccgcaa ttgggactat aacagtcgcc aaaaaagtga
     2941 aattcaattc aacatcagcc agacaatatt tgatggggta agtctgtatg cctccggttc
     3001 acagcaagac tattggggca ataacgagaa aaacaggaat atctctgttg gggtttccgg
     3061 ccagcaatgg ggaattggtt acagcctgaa ttatcaatac agccgctaca ctgatcaaaa
     3121 taatgaccgt gcactctctt tgaatctcag tattccgtta gaacgctggt taccgcgtag
     3181 ccgggtttcc tatcagatga ccagccagaa agatcgccca acccaacatg aaatgcgtct
     3241 tgatggttca ctgctggatg atggtcgcct gagctatagc ctggaacaaa gtctggatga
     3301 cgataacaac cataacagta gcgtgaacgc cagttaccgt tcaccttatg gaaccttcag
     3361 tgccggatac agttacggta atgacagtag ccaatacaat tacggcgtta ccggcggcgt
     3421 ggttatccat cctcatggtg tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat
     3481 tgatgctaac ggggcatctg gcgtgaggat acaaaactat ccggggattg ctactgatcc
     3541 ctttggctat gcagtggttc cttatctcac gacttatcag gaaaaccgtc tctcggtaga
     3601 tactacgcag ctgcccgata acgtcgatct tgagcaaaca acacagtttg tggtgcccaa
     3661 cagaggtgca atggtagcgg cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac
     3721 agtcagcgat cgcaacggta aaccgttgcc ctttggcgct cttgccagca acgatgagac
     3781 ggggcaacaa agtatcgtcg atgagggcgg catactatat ctctctggga tatcgagtaa
     3841 atcacaaagc tggactgtac gctggggaaa tcaggcagat caacaatgtc agtttgcttt
     3901 tagtacaccg gattcagaac caacaacctc tgtattacaa ggcacagcgc agtgccatta
     3961 aggataaaaa aatgaaaaga gcgcctctta taacaggact tttgttgata tccacatcct
     4021 gcgcttatgc ctcctcagga gggtgtggag ccgacagcac tagcggtgcg acaaattaca
     4081 gcagtgtggt tgatgatgtt acggtgaacc agacagataa cgtgacagga cgggagttta
     4141 cctctgcaac gctaagtagc actaactggc aatacgcctg ttcctgctct gcgggtaagg
     4201 cagttaaact tgtctatatg gtcagccctg tactcaccac cactggacat caggcaggat
     4261 attacaaact caatgatagc ctggatatta aaaccacctt aaaggcgaat gacattccag
     4321 gactcgtgac tgaccaaacc gtttctgtta acacccgatt cacacagata aaaagcaaca
     4381 ccgtatattc tgctgcaacc caaacgggtg tttgccaggg tgacacgtct cgttatggac
     4441 ccgttaatat tggtgcaaat accaccttta ccctgtatgt caccaagcca tttctcggct
     4501 cgatgaccat tccgaaaacg gatattgccg tcattaaagg cgcgtgggtc gatggaatgg
     4561 gaagcccgtc tacaggtgac ttccatgatt tagtcaagtt atcgattcaa ggaaatctca
     4621 ccgccccgca gtcgtgcaaa attaatcagg gcgatgttat taaggttaat tttggattca
     4681 tcaatggtca gaagtttacc acccgcaatg ccatgccaga cggttttact ccagtagact
     4741 ttgatatcac ttatgactgt ggtgatactt caaagattaa aaactcgttg caaatgcgca
     4801 tcgacggtac aactggggta gtagaccagt acaaccttgt cgccagacga agaagttcag
     4861 acaatgcgcc cgatgtcggt attcgtattg aaaatctcgg cggcggagtt gcaaatattc
     4921 cttttcagaa cggtatcctt cccgttgatc cttccgggca tggcaccgtc aatatgcgcg
     4981 cctggccagt taatctggtc ggtggtgagc tggaaacagg aaaatttcag ggcacagcca
     5041 ccattaccgt catcgtgcgg taaaagaaga cgttgcgttt tgattaacgc aacgcttcgg
     5101 cactcttacc cctgctgctc cagcgcatac ttatacagcg catttttctt cacgccatga
     5161 atctctgcgg ccagcgccgc cgcttttttc agcggtagtt ctgcctgtag cagcgccagc
     5221 gtgcgcaggg catcggcggg taagtcttct tcctgcgctt tatggccttc gacaatcagc
     5281 accatttcgc ctttgcgacg gttttcatct tctttgaccc acgccagcag ctcgccaacg
     5341 ggcgcgccgt gaatggtttc ccaggttttg gtgagctcac gcgccagaac cacgtagcgg
     5401 gattcgccta ataccgcaac gatatcttcc aggctgtcta acagacggtg ggttgattca
     5461 taaaaaatca gagtgcgcgg ctccgcttca atggctttta gcgcatcacg gcggcctttt
     5521 gatttggcag gtaaaaagcc ttcgtaacag aaacggtcag agggtaaacc cgcggcgctt
     5581 aacgcagtga tagcagcaca cggcccgggt agcggcacca cgcgaatccc cgcttcacgg
     5641 caggtacgca ccagatggta gccaggatcg ttaattagcg gcgttccggc atcggaaacc
     5701 agcgcaatgt tttgcccctc ttgcagcttc gccagcagcg tttcggcttt ttgttgttcg
     5761 ttatggtcgt gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag caataaaccg
     5821 gtgtgacgag tatcctcggc ggcaatcaga tcaacggcct gtaatacctc taacgcacgc
     5881 tgggtgatat ccgccagatt gccgattggc gtcggtacaa tgtaaagctg gccctgagaa
     5941 ttatccgccg attggtgttg tttcattgtg tcgtccgtat tgccgattta atattgagca
     6001 ttgcgtaaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     6061 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     6121 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     6181 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     6241 cgtgcactgg tgaaagaagg taaaaccggg caggcggtgg agttgtttaa ccaactaccg
     6301 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     6361 gcgcagaaag attttgctgg cgcgcaaaat ttgctggcga aaatcacacc tgccgattta
     6421 gaacaaaacc agcaagcccg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     6481 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     6541 cagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     6601 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     6661 tggtttgata accgaaacga tcccgacatg atgaaagccg ggatcgccga ctggcagaaa
     6721 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     6781 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     6841 gtatttggtc gcactattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     6901 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag cagaacaacc tcagccgcaa
     6961 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgaacag
     7021 cctgcagccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     7081 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     7141 atcttaagcc aggttcagca ggatggcgcg agtattgtgg tcggtccgtt gctgaaaaat
     7201 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagccg
     7261 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     7321 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     7381 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     7441 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     7501 ggcggttctg gtattgcttt aacgggtagc ccgattactc ccagagcgac aaccgactcc
     7561 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     7621 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     7681 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caacgctgta cgccagctcc
     7741 cgcagtgcgc aagggaccgc tggcccggat ttccgactgg agatggaagg cttgcagtac
     7801 agcgaaatcc cgatgctggc aggcggtaat ctgccgttaa tgcagcaggc gctcagcgcg
     7861 gtgaacaacg attattcgct ggctcgcatg tatgcgatgg gcgttgatgc ctggtcgttg
     7921 gcaaatcatt tctctcaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     7981 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     8041 caaggtcagg tagtccccgc cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     8101 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     8161 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     8221 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     8281 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     8341 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     8401 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     8461 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     8521 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     8581 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     8641 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     8701 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     8761 catgcgggag atgtattgtt agccatttcc acgcgtggca acagccgcga tattgtaaaa
     8821 gcggtagaag ctgccgttac gcgtgatatg accattgtgg cattaaccgg ctatgacggc
     8881 ggtgaacttg caggtttgtt agggccacag gatgtggaaa tccgcattcc ttcgcatcgt
     8941 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     9001 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     9061 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     9121 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     9181 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     9241 gcgcgcatta atgtaacggc ctatcagggc aaagtgctgc tggttgggca gtcaccaaac
     9301 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     9361 tataacgaga ttcgtcaggg ccaaccgatt ggtctgggcg aagcgtctaa cgatacgtgg
     9421 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     9481 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     9541 aaagcggcgg cagatattgc cagccgggta agcggcgtga aacgggtaac tacggcgttt
     9601 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     9661 gcctacaacg acacaaaacg taggtcagat aaggtgttta cgccgcatcc gactgctgtt
     9721 gcctgatgcg acgcttgccg cgtcttatca ggcctacaac gatacaaacc gtaggtcaga
     9781 taaggcgttt acgccgcatc cgacatgttt tccctcaaat cagaacaaca gcgccagccc
     9841 gccgacaatc acaccagaca ctgccaccat cgcccccgtc agccataagg ctttcgccgg
     9901 gaacgctttg cgcagcataa tcagtgacgg caaactcacc gccgggagcg tcatcaacaa
     9961 tgccagcgcc ggagcagttc ccatacctgc cagcatcatc gtttgtacaa tcggaatttc
    10021 tgctgccgtg ggaatgacaa acaagcatcc tgccaccgcc atcgccacca cccacatcag
    10081 gctgttatcg acagtaccat cggcatgggg gaataaccag acgcgagcgg cacccaacac
    10141 cagtactgca aggatgtaaa ccgggatcgt actccagaaa agcgtccata gcgccctgcc
    10201 ccagcggcta aaaaaaccgc cctgtgcttc cggtatgtca atttcgaccg gtgcctgcgt
    10261 ttgcggtgtt tcacgcaccc atttttgcac cagcgtcgca atcagcaaca ccatcaccag
    10321 cccggccacc agacgaatcg ccgcaaaacc ccagccgagg acaaagccca taaacaccag
    10381 cgtcgccggg tttaacaccg gattgcccat ccagaatgcc agcgcaccgc ccatcgacac
    10441 ctgttggcga cgcattcccg ccgcgaccgg agccgcacag caggtacaca tcatgcccgg
    10501 caacgaaaac agcgttccca gcagcgtgcc gcgaaagcgc gattgcccaa gcgtacgcaa
    10561 caaccagtca cgcgggatca gcacctgaat caacgaaccg aggatcaccc ccagcaccgc
    10621 cgctttccat accgcgagga aatagatcat cgcgtaatcc aacgccgcct gccatgggtt
    10681 agcatccgcc tgcgcaagga tagatttacc gatactgtgg gtttcggcag cagtaaacgc
    10741 tttgccgtag taaggttccc atttcacata ccagagaccg gcaatgacaa cgagaaagaa
    10801 aagcgcgggt ttccaccact gaatgggcgt tgccgcctga gatgaagact gaccagtcat
    10861 agcattcccc aggaataagt tatgataatt gagcgcgtga atattacgct cactatcaat
    10921 tcttgggaat aattattcag ctcttttgcg taattctgaa gagcttaaaa tcgtcacgcc
    10981 ttcatgctca ggccgcatcg actctgccag catgacccgc gccacgtctc tcgcatcaat
    11041 ggatttccag ttacctggta acaaacggaa cagcggcgca aaaagcgttt cgttcatccg
    11101 ctgtttgcta cgatcgccca gtaacatcga cgggcgagca atggtcaatt tcggccagtt
    11161 ctgggcaatt aatgcttctt ccatctcccc tttgacgcgg ttatagaaaa acggcgagtg
    11221 ggcattggca cccatagcac tgaccaccag catatgctgc gcgcccagcc gccgcccggt
    11281 taatgcggta tccactacca gcgtgtaatc ggcatgaata aacgcctctt tgctccccgc
    11341 ttctcgccgc gtggtgccga gacaacaaaa cacaatgtcg atgggatcgg tgacctgcgc
    11401 cagcgcgtca gtcagttgcg gatcatgggg attgaacact cctggcatat ctcccaacgg
    11461 acgtcgcgtc ggtgcagcaa tagcgttaac tttcggttcg ttaatcaaca tccgcagcag
    11521 gtgaccgccc accagccccg ttgcgcctgt aatcagtacc tgactcatct tcgctccttt
    11581 acagaattgt ctgccttgcg ctccccggct catgcaccag gcttaataga ccatgaggta
    11641 attatccccg attgtgagaa attcgcctca tccaatgcaa caacgcggag gaagcatgag
    11701 taagaaaatt gccgttttaa tcactgatga atttgaggat tcagaattta cttcacccgc
    11761 agacgagttc cgtaaagccg gacacgaagt gattaccatt gaaaaacaag cgggtaaaac
    11821 ggtgaaaggc aaaaaaggag aagccagcgt gaccatcgat aaatccatcg atgaagtgac
    11881 gccagcggag tttgatgccc tgttgctacc gggcggtcat tcaccggatt atctgcgtgg
    11941 cgacaaccgt tttgtcacct ttacccgtga ttttgtgaat agcggcaaac ctgttttcgc
    12001 catctgtcac ggtccgcagt tgctgatcag cgccgatgtg attcgggggc gcaaactgac
    12061 cgcagttaaa ccgatcatta ttgatgttaa aaatgcgggc gcggaatttt acgatcagga
    12121 agtcgtggtt gataaagatc agctggttac cagccggaca ccggacgatc tgccagcgtt
    12181 taaccgcgag gcgttacgcc tgctcggtgc ctgagtcgcg tagccaaatc atttttttac
    12241 caaagcccag cgtgttgtcg gtgaatttga tttcatcgag ccggatttcc cacaccgggg
    12301 ccgacagcat tctggcaacc ggaaagcgac gattgtacgc cttgcgcgcg aggtcgcttt
    12361 cctcaccttc cagcctgcgg atctcacctt taaactgcac accgcgaatt aacgctaccg
    12421 ttttcggctg accgtttacc gttccggcaa ctgccgcctg cggcccgctc atctgcgcgt
    12481 ggcgcgtttt ttcttccgtc aaaatgtaga aggcgacttt ctgcgcatca aaaagataaa
    12541 aggcattagc gcaccagagt tccccttcct gctgcacaca ccaggtgaca acatgttgtt
    12601 ttgccagcca acggctgatg gcgatgagtg tttccattgc tgttctcttt tatactgtgg
    12661 gcctgacctt aacatgcgat gcagacgatg acaccctggt ttctttactt gatccgtacc
    12721 gccgacaata agctttatac cgggatcacc acggatgtcg aacgccgcta tcagcagcac
    12781 caaagcggca aaggggcgaa agcactgcgc gggaaaggag aactgacgct ggcgttttct
    12841 gctccagtgg gcgatcgttc gctggcgtta cgggcggaat atcgcgttaa gcaactgacc
    12901 aaacggcaaa aagagcgtct ggtagcggaa ggcgcagggt ttgcagagct gttaagcagt
    12961 ctgcaaaccc cggagattaa aagcgattga aatgctcgtg atactcaacc aggccggtaa
    13021 cgccattcag cgcgtcatcc gctaaacgat gtacctggaa ggcgctttca gtgcccggcc
    13081 agcggcaacg cagatcgtga tgcgccgcca gttcaaagcc gaaacgactg tacagcgccg
    13141 gatcgcccag cgtcaccact gcggcatagc cgaactcatt aagcgaatcg agtccttcat
    13201 agaccagttg gcgtgccagc ccctgtccac ggtatttttc atcaaccgcc aacggtgcca
    13261 tgccgaccca ttgcaggtct tcgccctgca catcaaccgg actaaatgcc acatagccaa
    13321 tgacctgacc ttcgtcatct gtcgccacca gccccagcgt cagaaagcca tcttcacgca
    13381 gatcgtgaac cagcttcgct tccgcatcac tttcgaatga gcgacgcagc agggcatcaa
    13441 tacccggcgc atcaatggga atttctactc gaattagcat ggttcaccta ccgatgtctg
    13501 tttggtttca ggcgcggttt tcattcccgc ctcaacaaaa tccgccagtt gcagcagcat
    13561 catgcgtaag gctttcggca tttgctccag ttcaatggcg tccatcaggt ttttcacata
    13621 cagccccagc tccgtatcgc cttcaatcac cagccgacgc tggaagaaga gcgtatccgg
    13681 atcttgttta cgcgccgcaa tcatcagcag atcgctggcg tcggcactaa aactcacatc
    13741 agcttgcgcg ttctggctga ccaccagttt gccattcacc accgaggtaa accattgcag
    13801 gtcaatatca cgcacatgaa tacttaacca gcggccttca agaaactcca gctcgccatc
    13861 atccagcgcc tgacggaatt gccagcttaa gacctgctca agaacctggc gttttagcgc
    13921 aaatggcgtc agttttaccg gtacactcaa cagagatggc cccaaatgca caatacggga
    13981 acgcagttta tccaacacga gttttactcc ctgtttcaac aatcatccta ttttgccata
    14041 tcagaaaaat aacatagcgg tataaatcaa caattccata tgaaattgct gctaccacca
    14101 atacaacttt aactgcctta aatcaaaaat tgtcgcagca aggttaacta aaatcccagt
    14161 tcgttaacat ttttgcgttt tgatagcgca accttcagga aaaattatgg agctgctctg
    14221 ccctgccgga aatctcccgg cgcttaaggc ggccatcgaa aacggcgcag atgctgttta
    14281 tatcgggcta aaagatgata ctaatgcccg tcacttcgcc ggccttaact ttaccgagaa
    14341 aaaattgcag gaagcggtga gttttgtcca tcaacatcgc cgcaaacttc acatcgcgat
    14401 taacactttt gcgcatccgg acggttacgc ccgttggcag cgcgccgtgg atatggcggc
    14461 gcagctgggt gccgacgcgc tgatcctcgc cgacctcgcc atgctggagt acgccgccga
    14521 gcgttacccg catattgagc gtcatgtgtc agtgcaggct tcggcgacca atgaagaggc
    14581 gattaacttt tatcatcgcc attttgacgt tgctcgcgtg gtgctgccgc gcgtgttgtc
    14641 gattcatcag gtgaaacagc tggcacgggt tactcctgtc ccgctggaag tgtttgcttt
    14701 cggcagcctg tgcattatgt cggaaggtcg ttgctatctg tcgtcgtatc tgacgggtga
    14761 gtcgcccaac accgtgggag cgtgttctcc ggcccgtttc gtgcgctggc aacaaacgcc
    14821 gcaggggctg gaatcccgcc tgaacgaagt gctgatcgac cgttatcagg acggcgaaaa
    14881 cgcaggttat ccgacgctgt gtaaagggcg ttatctggtg gacggcgagc gctatcacgc
    14941 gctggaagaa ccaaccagtc tcaataccct ggaactgctg ccggagttaa tggctgcgaa
    15001 tattgcttcg gtgaaaattg aaggccgcca gcgtagcccg gcgtatgtca gccaggtggc
    15061 gaaagtctgg cgtcaggcta tcgaccgttg taaggccgat ccgcaaaact ttgtaccgca
    15121 aagcgcgtgg atggagacgc tcgggtcgat gtccgaaggc acgcaaacca ctcttggcgc
    15181 atatcaccgt aaatggcagt gagaaaagca atgaaatatt ccttagggcc agtgctgtgg
    15241 tactggccaa aagagacgct ggaagaattt tatcagcagg ccgccaccag cagcgccgac
    15301 gtgatttatc ttggtgaagc ggtatgcagc aagcgtcggg caaccaaagt tggcgactgg
    15361 ctggagatgg caaaatcgct cgccgggagt ggtaagcaga ttgtgctctc cacgctggcg
    15421 ctggtgcagg catcatctga actgggcgaa ctgaaacgct atgttgagaa cggtgagttt
    15481 ctgattgaag ccagcgatct cggcgtggtg aatatgtgcg ctgaacgcaa actgccgttc
    15541 gtcgccgggc acgcgctgaa ctgctacaac gcggtgacac tgaaaatatt gctcaaacag
    15601 ggcatgatgc gctggtgtat gccagtggag ctttcccgcg actggctggt gaatctgctt
    15661 aatcagtgcg atgagctggg cattcgtaac cagtttgaag tggaagtcct gagctacggt
    15721 catctgccgc tggcctactc cgcccgctgc tttaccgcgc gttcggaaga ccgcccgaaa
    15781 gatgagtgtg aaacctgctg cattaagtat ccgaacgggc gcaacgtgct gtcgcaggaa
    15841 aaccaacaag tgtttgtact caatggcatt cagaccatga gcggctacgt ttacaacctc
    15901 ggtaacgagc tggcatccat gcagggcctg gttgatgtgg tccgcctgtc accgcagggt
    15961 actgacactt tcgcgatgct cgacgccttc cgcgctaatg aaaatggcgc tgcaccactg
    16021 ccgttgacgg cgaacagcga ctgtaacggc tactggcggc ggctggccgg gctggagctg
    16081 caagcctaag taaatatgct cgtcatactt caagttgcat gtgctgcatc tgcattcgtt
    16141 caccccagtc acttacttat gtaagctcct ggggattcac tctcttgtcg ccttcctgca
    16201 actcgaatta tttagagcac agctcacttt gttaacaact ttaactactc tttaatgcag
    16261 tattaaagat taatcggtaa caaagtgagc tgttatgact gataaaacca ttgcgttttc
    16321 gctactcgat ctggccccca ttcccgaagg ttcttcagcg cgagaagcat tctcccactc
    16381 tctcgatctc gcccgtctgg ctgaaaagcg cggctatcat cgctactggc tggcggaaca
    16441 ccacaatatg accggcattg ccagtgctgc aacatcggta ttgattggct atctggcggc
    16501 gaataccacc acgctgcatc tggggtctgg cggcgtgatg ttgcctaacc actcaccgtt
    16561 ggtcattgcc gaacagttcg gcacgctcaa tacgctctat ccggggcgaa tcgatttggg
    16621 gctgggtcgt gctccgggta gcgatcagcg aaccatgatg gcgctacgtc gtcatatgag
    16681 cggcgatatt gataatttcc cccgcgatgt cgcggagctg gtggactggt ttgacgcccg
    16741 cgatcccaat ccgaatgtgc gcccggtacc aggctatggc gaaaaaatcc ccgtgtggtt
    16801 gttaggctcc agcctttaca gcgcgcaact ggcggcgcag cttggtctgc cgtttgcgtt
    16861 tgcctcacac ttcgcgccgg atatgttgtt ccaggcgctg catctttatc gcagcaactt
    16921 caaaccgtcg gctcgactgg aaaaaccata cgcgatggtg tgcatcaata ttatcgccgc
    16981 cgatagcaac cgcgacgctg agtttctgtt tacctcaatg cagcaagcct ttgtgaagtt
    17041 gcgccgtggc gaaaccgggc aactgccgcc accgattcaa aatatggatc agttctggtc
    17101 gccgtccgaa cagtatggtg tgcagcaggc gctgagtatg tcgttggtag gtgataaagc
    17161 gaaagtgcgt catggcttgc agtcgatcct gcgcgaaacc gacgccgatg agattatggt
    17221 caacgggcag attttcgacc accaggcgcg gctgcattcg tttgagctgg cgatggatgt
    17281 taaggaagag ttgttgggat agtgtgtctt aacgcgggaa gccttatccg agctggcaac
    17341 gctgtcctac gtagacctga taagcgaagc gcatcaggca ttgtgtaggc agcagaaatg
    17401 tcggataagg caccgctgat tactgataca ccggcagtaa attaaagctc gataaaatat
    17461 ggaccagtgc gttgccaacg ccaaacacca gaatcagcgt aatcatcggc ttgccgcccc
    17521 agacgcggaa tttcgggctg ccaaagcgtt tacgcgatgc gcgggctaac agcgccggaa
    17581 caattgccgc ccagatggta gctgctaaac cagcataacc aatggcgtac aggaatccgt
    17641 tcgggaacag tagaccaccc acaacaggcg gggcaaaggt cagcaatgcc gttttcaagc
    17701 gccccagggc tgagtcatcg aaaccaaaca gatctgccag atagtcaaac aaacccagcg
    17761 ttacgccaag gaacgaactc gctaccgcaa agtttgagaa cacgaccagc agaaggtcca
    17821 ggctacggct gttcagcacg ccgcttaatg cctgtaccag cacatcaata ttaccgccct
    17881 tctctgcaat accgataaac tccggacgcg ggatgttacc catcgtcgcc agcaaccaga
    17941 tggtatacag cgccagtgcc atcagcgtac cgtacaccag gcatttcacg atggttttcg
    18001 gatctttgcc gtaatacttc atcaggcttg gcacgttacc gtgataacca aacgacgcca
    18061 ggcagaacgg caatgtcatc agcagatacg gcgcataaga tgcattgctt tctgcaacgt
    18121 tgaacaatgt cgcaggctgc acatgcccca acaggctgcc aaaggtgagg aagaaggtaa
    18181 tgactttcgc ccccagcaca atcgccgtca tgcggctgac ggccttagta ctcaaccaca
    18241 ccacaaacgc taccagcagt gcaaaaccaa aacccgccgc ccgtgccggg acgtttagcg
    18301 acatctctgc gaaggtgtga tgcagaatcg aaccactggc agaaatatag gcataggtca
    18361 ggatatagag cacaaaggca atggaaatac cgttgaccac gttccagcct ttgcccagca
    18421 aatctttggt gatggtgtca aaactcgaac cgattcggta attcaggtta gcttccagaa
    18481 tcatcaagcc ggaatgcagc atacagaacc aggtaaagat cagcgccgcc attgaccaga
    18541 aaaaccacgc cccggacatg accactggca gagaaaacat ccctgcgcca ataatggtgc
    18601 cgccgataat caccacgccg ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg
    18661 ccatgagggc ttctctccag tgaaaaatag tgcgactgcg ttgttatgca ttgcactgta
    18721 ccagtacacg agtacaaaag acagaaaaaa agccccgatg gtaaaaatcg gggctgtata
    18781 tattatttta cagattgtgt tcgctgttca gcgatgatta cgcatcacca ccgaaacgac
    18841 gacgaccggt agagtcatca cgacgcggag cgcggccttc acgacgttcg ccgctaaaac
    18901 gacgaccatc accacggcca ccttcacggc gttcaccgct gaagttacga ccgccttcac
    18961 gacgttcgcc accgaaacca cgaccaccgc cacgacgctc accgccagta tgcggctgtg
    19021 catcgcccag taactgcatg ttcatcggct tgttgagaat gcgagtgcgc gtaaagtgtt
    19081 gcagcacttc acccggcata cctttcggca gttcgatggt ggagtgagaa gcaaacagct
    19141 tgatgttacc aatgtaacgg ctgctgatgt cgccttcgtt agcaatcgca ccaacgatat
    19201 gacgaacttc aacaccatca tcgcggccca cttcaatgcg gtacagctgc atatcgccaa
    19261 catcacgacg ttcacgacgc ggacgatctt cacggtcacc acgcgggcca cggtcgttac
    19321 gatcgcgcgg accacggtca tcacggtcac ggaattcacg tttcggacgc atcggcgcat
    19381 ctggcggtac gatcagagta cgttcaccct gtgccatttt cagcagtgcc gcagccagag
    19441 tttcgagatc cagctcttca ccttctgcag tcggctgaat tttgctcagc agtgcgcggt
    19501 attgatccag atcgctgctt tccagctgct gctgtacttt agcggcgaat ttttccagac
    19561 ggcgtttgcc cagcagttct gcgttcggca gttctacttc cggaatagtc agcttcatag
    19621 tacgttcaat gttgcgcagc agacgacgct cgcggttctc aacgaacagc agcgcgcggc
    19681 cagcacgacc cgcacgaccg gtacgaccga tacggtgaac gtaagactca gaatccatcg
    19741 ggatatcgta gttaactacc aggctgatac gctcaacgtc caggccacgg gctgcaacgt
    19801 cggtcgcaat caggatgtcc agacgaccat ctttcaggcg ttccagtgtc tgttcacgaa
    19861 gcgcctggtt catgtcaccg ttcagcgcgg cgctgttgta gccgttacgc tcaagagctt
    19921 cagccacttc cagagtcgcg tttttggtac gaacgaagat aatcgccgca tcaaaatctt
    19981 ccgcttccag gaaacgtacc agtgcttcgt ttttgcgcat accccagaca gtccagtagc
    20041 tctggctgat gtcaggacgg gtagtcacgc tggactgaat gcgcacttcc tgcggctctt
    20101 tcataaagcg gcgggtaatg cgacgaatcg cttccggcat ggttgcagag aacagagcgg
    20161 tctgatgacc ttccgggatc tgcgccataa tggtttcaac gtcttcgatg aagcccatgc
    20221 gcagcatttc gtcagcttca tccagaacca gaccgctcag tttagagagg tccagagtgc
    20281 cacgtttcag gtggtccagc agacggcccg gagtaccgac aacgatctgc ggcccctgac
    20341 gcagggcgcg taattgcacg tcataacgct ggccgccgta cagagcaacc acatttacgc
    20401 cgcgcatgtg tttagagaaa tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg
    20461 tcggtgccag caccagaatc tgtggtgctt tcagctcagg atcaagattc tgcaacagag
    20521 gtaaagagaa tgctgcagtt tttccgctcc ccgtctgggc catacccaga acgtcgcggc
    20581 cattcagcag atgtggaata cactctgcct gaattggaga tggtttttcg taacccagat
    20641 cgttaagggc ttcaaggata ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt
    20701 cagccatgta gtacgtgtgc ctcaaaatta atggcggcca gtctacataa ctcatcatga
    20761 aattgatcag caattttcat tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa
    20821 caacaacgcc ctcacccgtt aaggtgatgg caatcaaaaa agattacggg ctgatgtgta
    20881 cgtcagctat tgctggtccg attctgccag gtcatcttgg tcctggccca ggagcgataa
    20941 ttccaacaat gcgtatcggt gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt
    21001 gaacagtgcc gtggcgctgt ccaaatcccc cagacttagg tagtacttac ctaaatagaa
    21061 gttggtttca ctgagatgct cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct
    21121 ttccattaac gtttgttcgc taatgttgcc caggtagaac tcgacaatgt tccatcccca
    21181 ctgttcctta tccgattttt cgaagtgctg tttcaacact tctttagcct gcttctcatc
    21241 gagcttctgc tcggcgagat aaagccacag actacggaaa ggatcattgg gatcgtcttg
    21301 ataaaacgcc agcagatcat cttgcgctaa cttgtcacga ccgccgtaat ataatgcgat
    21361 cccgcgattc aagtgcgcgt agttgtaagt tggatcaagc tcaagtacag aatcaaacgc
    21421 ttcataggca gcatcaaaat tgcctgcctg cgttaaatat atgcctaagt aattgaatac
    21481 ttcaggcata tccggtcgga ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct
    21541 cagaccgaga ctatcataca acactccgcg ctcatataaa agctgtgcgc gttcgtcatc
    21601 ggttaaagcc cgactggcaa ggatttgttc catacgtgcc agaatcactt cctgctgtaa
    21661 agtcggttgc aatggtaccg cgaggacttc acttttacgc caggaagtat tactgcatcc
    21721 tgcaagcgta agtgctgtcg caacgaaaca ccagcgcaaa aaaggcttca tttcccactc
    21781 ccgaagacca cggttgaatg aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg
    21841 ttaaaagccc cccgccgcag cggagggcaa atggcaacct tactcgccct gttcagcagc
    21901 cggagcttcc ggtgctgcag caggttgaga ctgctcagtc gcttctttaa tgctcagacg
    21961 gatacggccc tggcgatcaa cttccagaac tttcaccggt acttcctgac ccatctgcag
    22021 gtaatcggtc actttctcaa cgcgtttgtc agcgatttga gagatgtgga ccagaccttc
    22081 tttaccgccg ccgatggcaa caaatgcgcc aaagtcaacg atacgggtca ctttaccagt
    22141 gtagacgcgg cccacttcga tttctgcagt gatctcttcg atacgacgaa tagcatgttt
    22201 cgctttctcg ccgtcggtcg ctgcgatctt cacagtaccg tcatcttcga tttcgatggt
    22261 agtgccagtt tcttcggtca gggcacggat aacagagccg cctttaccga taacgtcttt
    22321 gatcttatcc gggttgatct tgatggtatg gatacgcggt gcgaactcag agatatcgcc
    22381 acgcggcgcg ttgatcgcct gttccattac gcccaggata tgcagacgcg cacctttagc
    22441 ctggttcagc gcaacctgca tgatctcttt ggtgatacct tcaattttga tatccatctg
    22501 cagtgcagag ataccgtcgc gggaacctgc aactttgaag tccatatcgc ccaggtgatc
    22561 ttcgtcgccc aaaatgtcag acagtacaac gtagttgtcg ccttctttca ccagacccat
    22621 tgcgataccc gcaacggcag ctttgattgg cacacctgcg tccatcagcg ccagagacgc
    22681 gccgcacacg gaagccatag aagaagaacc gttggattcg gtgatttcag acactacacg
    22741 tacggtgtac gggaatttgt ccatatccgg catgactgcc agcacgccgc gcttcgccag
    22801 acgaccgtga ccaatttcac gacgcttcgg agaaccgacc atgccggttt cgcctacgga
    22861 gtacggaggg aagttgtagt ggaacaggaa ggtatcggta cgttcgccca tcagttcatc
    22921 aagaacctgc gcgtcacgag cggtacccag cgttgcagta accagtgcct gcgtttcacc
    22981 gcgggtgaac agcgcagaac cgtgagtacg cggcagcacg ccagtacgca catccagacc
    23041 acggatcata tctttttcac gaccgtcgat acgcggttcg cctgccagta cgcggctacg
    23101 aacaacgttt ttctcgatag cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc
    23161 gtcttcagca agcagcgtcg cgatggtttc agatttgatg acatcaacct gcgcataacg
    23221 ctcttgtttg tcggtgatgc ggtaagcatc gctcaggcga gcttcagcca gtgcagcaac
    23281 gcgcgcgttc agcgcttcgt ttaccggctc cggctgccag tcccaacgtg gtttaccggc
    23341 ttctttcacc agttcattga tgttctgaat aacaacctgc tgttgttcat gaccgaacac
    23401 tactgcgccc agcatctggt cttcgctcag cagttcagct tcagattcaa ccatcagaac
    23461 agcggcttca gtaccggcaa caaccagatc cagtttgctc tctttcagct cgtcctgagt
    23521 cgggttcagt acgtactggt cattgatgta acctacgcga gcagcaccaa tcggaccatt
    23581 gaacggaata ccagacagag acagtgctgc ggaagcacca atcatcgcga cgatatccgg
    23641 gttaacttgc gggttaacag aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc
    23701 ttccgggaac agcgggcgaa tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc
    23761 gcttgggcgg ccttcacgac ggaagaagct acccgggata cgaccagcag cgtaggtacg
    23821 ctcctgatag ttaacggtca gtgggaagaa gtcctgacct ggtttggctt ttttctggcc
    23881 aacaacggta acaaataccg cggtgtcatc catgctaacc ataacagcgg cagtagcctg
    23941 acgagccatc atgccggttt ccagagtcac ggtgtgttgg ccgtactgga atttacgaac
    24001 gatcggatta agcaatgtaa tatcctttct ctttcttaga cagtacctta cggcactggt
    24061 gttaataccc gatcttctgc gcatcctcgc gactaatgac aaccctaacc cagctctatg
    24121 tgggtaaagc ctctcattag ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca
    24181 atacattagt ttccagtgaa ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc
    24241 cttttctgaa actcgcaaga attagcgacg cagacccagg cgctcgatga gctgggtgta
    24301 acgtgctacg tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg
    24361 cagcagacca cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg
    24421 gttgatctgt gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc
    24481 acgaccaaac tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact
    24541 ccaaagtata tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac
    24601 gcaaagtgtt aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacctgt
    24661 tatcgcaaga cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcaacgc
    24721 ggccttcatc gtcaatttcg cccataccga taaatttgcc gttctcacct tctgtaacgc
    24781 gaaccagtcc ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa
    24841 cagaagacgt taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca
    24901 tcagtaatgg atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca
    24961 gttcacgcag atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac
    25021 gacgcaggta aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg
    25081 tgcggatata agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc
    25141 gaataaacag caattcataa acggtaatcg ggcgcgcttc acgcggtact tcaatgccct
    25201 gacgcgcata ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga
    25261 tctgttcgat atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg
    25321 ttaccggacg ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa
    25381 gacgcgcaat gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg
    25441 cttccccgag gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc
    25501 cggcacggtt ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac
    25561 cctgcggttt atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac
    25621 tcattagtcc tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac
    25681 gctggtcacc aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt
    25741 cagttccggc acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga
    25801 agcttcttgc aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa
    25861 cgtcacatat actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt
    25921 catcatgccc aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg
    25981 catttcctgc gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga
    26041 caaaaaaggg gctgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt
    26101 tggcctgata agacgcgcca gcgtcgcatc aggcaatcca tgcaggatgt ggcgtaaacg
    26161 ccttatcccg catggaaccc taaaaacctt aagcgatggt acgttggatt tcgatgattt
    26221 cgaatacttc gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac
    26281 attccatacc gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct
    26341 cgccttcgta gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca
    26401 caccttcggt aaccatacag cctgcgatgg caccaaattt cggtgatttg aacacgtcac
    26461 gaacttccgc cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg
    26521 ccgctttcac ttcgtcaatc aggttataga tgacggagta gtaacgcaga tccaggcttt
    26581 ccgcttcaat cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg
    26641 cgttggacgc cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga
    26701 tgatcttcac tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca
    26761 cagaaccctg tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca
    26821 tgttggcgaa catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga
    26881 atttaccctg acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa
    26941 cttcatcacc cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac
    27001 ccgcttccag cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga
    27061 agccacacag aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa
    27121 ccggaccacg acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac
    27181 gtaccgcttt cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga
    27241 taccagtacc cgctttcgca gacacatgta cgaactggct ttcaccgccc cactcttccg
    27301 gcaggatgcc gtactgggag agttcgttct taacgcgatc cggatcggct tctggtttat
    27361 cgatcttgtt cactgcaacg accaccggaa cctgcgccgc tttcgcgtgc tggattgctt
    27421 cgatcgtctg cggcattacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg
    27481 cctgcgcacc acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg
    27541 tgatcatgcc gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg
    27601 cttcgccaga ggccactttc gttgaacgaa tgtagtccag cagagaggtc ttaccgtggt
    27661 caacgtgacc catgatggtc acaaccggcg cgcgcggttc agccgcagca cccgtgtcac
    27721 ggtcgctcat taccgcctct tccagctcgt tttcacgacg caggataact ttatggccca
    27781 tctcttcagc aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg
    27841 cgcccagttt catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca
    27901 gttcgccaac ggtgatagtt tcgccgatca caacgtcacg gttaacggcc tgagcaggct
    27961 tctggaagcc ttgctgcagg gaagaacctt tacgttttcc gcctttaccg ccacgtactg
    28021 ctgcgcgtgc ttcttcacga tcagcttttg attcagcgtg tttgttacct ttcttcggac
    28081 gcgctgcttt cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt
    28141 cttcggcctg gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg
    28201 gttccgcgtt atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc
    28261 gacgtgcttc ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg
    28321 cagcttcctg ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt
    28381 gattgctcac tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct
    28441 tagcttgttc tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt
    28501 ctgcctcacg acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gctgcaaggc
    28561 gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact tcgatttgta
    28621 ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt ttacgctgca
    28681 gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa gtctgtttct
    28741 cttgtgcaga cacagagtcg tcagcagact tccggatacc tgcatcagca aattgctgta
    28801 ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt acatctgtca
    28861 tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa atattacggg
    28921 cagccataat cagtgctccg gctttttcgt cggtcaaccc ttcgatatca gccagatcat
    28981 caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc agtttgaatg
    29041 ccaaatcacg atctacccct tcaaggttca gcagatcgtc agccggtttg ttatcaccga
    29101 ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg
    29161 cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc ggcacatagg
    29221 ccagttcttc cagcgtcgag aagccttctt ctaccagaac agtcgcgaag tcttcgtcga
    29281 tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct
    29341 gcaggtcgtc aacggtcatc acgttgagtt cccaaccgct cagctgcgaa gccagacgca
    29401 cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca
    29461 tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc attgcgttaa
    29521 tcacaaactg cgccgggtta tcatcccaca ggacgatatc gatacgctcg ccgcccagtt
    29581 cagtagaaac cgcctgaaca cgcgcgccac gcatacctac acaggcacct accggatcga
    29641 tacgtttatc gttggttttc accgcgattt tcgcacgaga acccggatcg cgagccgctg
    29701 ctttaatttc aatcacttct tcgccgattt ctggcacttc aatacggaac agttcgatca
    29761 gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc gggcgaacgg
    29821 aatagagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc agcatatctt
    29881 cgcgcaggat cacggcttca gcattgttgc ccagatccag agagatgttg tcgcggttta
    29941 cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga tcaaccacca
    30001 tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc tgggtagtga
    30061 tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccagg ttcaggcttt
    30121 catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg acttcatcaa
    30181 caactaacca gcgacggaag gtgtcaaaat caccactttt gcgatcgatc tgtacgcgga
    30241 cgtcgatctc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt
    30301 cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact acagccaaaa
    30361 tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg ggaaccaggt
    30421 tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg gtaactgtga
    30481 tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt tgtaccgcca
    30541 tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca gccgtgaaca
    30601 gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg atgggatctt
    30661 caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca acattgatgc
    30721 catcttcact atcaatatag atgcgcagtg tggatgtgcg accgcgaata aattcgatgc
    30781 caaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt aatttttgct
    30841 ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc agttattctg
    30901 tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac cctataaccg
    30961 caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac gaaggccgaa
    31021 gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg gccggatttg
    31081 aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgcctg
    31141 aaacgtggca aattctactc gttttgggta aaaaatgcaa atactgctgg gatttggtgt
    31201 accgagacgg gacgtaaaat ctgcaggcat tatagtgatc catgccacat tttgtcaacg
    31261 tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa tacttttgcc
    31321 ttcccctgct ctcccttaag cgcattattt tacaaaaaac acactaaact cttcctgtct
    31381 ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca gtgagagcgg
    31441 aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga cgattctcaa
    31501 gcatctcccg gtaggtcaac gtattggtat cgctttttcc ggcggtctgg acaccagtgc
    31561 cgcactgctg tggatgcgac aaaagggagc ggttccttat gcatatactg caaacctggg
    31621 ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat acggcgcgga
    31681 gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg ccgctattca
    31741 gtgtggcgca tttcataaca ccaccggcgg cctgacctat ttcaacacga cgccgctggg
    31801 ccgcgccgtg actggtacca tgctggttgc tgcgatgaaa gaagatggcg tgaatatctg
    31861 gggtgacggt agcacctata aaggaaacga tatcgaacgt ttctatcgtt atggtctgct
    31921 gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta ttgatgaact
    31981 gggcggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact acaaaatgtc
    32041 tgtcgaaaaa gcctactcca cagactccaa catgcttggt gcaacgcatg aagcgaagga
    32101 tctggaatac ctcaactcca gcgtcaaaat cgtcaaccca attatgggcg tgaaattctg
    32161 ggatgagagc gtgaagatcc cggcagaaga agtcacagta cgctttgagc aaggtcatcc
    32221 ggtagcgctg aacggtaaaa cctttagcga tgacgtagaa atgatgctgg aagctaaccg
    32281 catcggcggt cgtcacggcc tgggcatgag cgaccagatt gaaaaccgta tcatcgaagc
    32341 gaaaagccgt ggtatttacg aagctccggg gatggcgctg ctgcacattg cgtatgaacg
    32401 tctgttgacc ggtattcaca atgaagacac cattgagcag tatcacgcac atggtcgtca
    32461 gttgggccgt ctgctgtacc aggggcgttg gtttgactcc caggcgctga tgctgcgtga
    32521 ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttaccctgg agctgcgccg
    32581 cgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca agccagaacg
    32641 tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtattg gtcaattgac
    32701 catgcgcaac ctggatatca ccgatacccg cgagaaactt ttcggttatg ccaaaactgg
    32761 cctgctctcc tcctctgcca cttcaggcgt gccgcaggtg gagaatctgg aaaacaaagg
    32821 ccagtaattc gacatcaacc ctgcccttca ggggtggggt taccttgctt atcctctgta
    32881 aactcctttc tgagcatcac ataatcgaaa acatcatggt ttgcatccac aacccgtgag
    32941 tctcctttat agtgtgcaat cacttcctca agcgtttgcg gctgttccgg ctttgttacc
    33001 cccatccacg cattaataag gtaattattg taagctgtcg aaaagatgtt gttttccgtt
    33061 gtgcgatcca cgccatcccc aagaacgggg ctataccaga taaacatcgg gacatgatat
    33121 gcctgctggc tagcctccct gcctccatga aaatagacgt tcttcttcgt agggtcacgt
    33181 tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa taattcaaaa
    33241 acctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc atcctgatcg
    33301 tcctgcggtt gaaacacggc gctggattgc ggataggcgc tacaagccgg ttcatggctt
    33361 ccatttaaat gaagaacaat cagtttcttt tgctgcgtat tttgctgtaa tgcttgcgat
    33421 aaatgcggca acaacaattc atcaaatcct ctgacataaa ctgtttccat ggcgcgcatg
    33481 gcgatactgg taactgctgt accattctgc cgaaaagcgg attgcgagct tagccagaaa
    33541 gtctgaaatc ctgcttgatt agccatatta ataatgttgt cggggtaatt atgaatgtca
    33601 tgactcaaaa cagaatcagc agttaaagaa aggggaaccg acagcgcagt gtaaggtgcg
    33661 ccgcttattg cttgattaaa cagtttgatc tgttttcttt gtgcttcaac ttgcggtgtc
    33721 gtagagcgtg tatatccata taaagacata ttgtcgacac gtacagactc ccccacaatc
    33781 aacacgtagg tatcaatacc tgtatccctg actgataatt gaaaatacgg caccgtgttt
    33841 gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaatagtt gagattgaaa
    33901 aatggcgtat aggtagcaaa tcgcgacgct agtatatatg gactgaacgc ttttttattt
    33961 tttgcatctt tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat gacaatcagc
    34021 aataatattc ctgtcacttt ttttgtcggc aaggaaacat catattttat tattactgcc
    34081 aaaaaaagaa gggataaaaa agcaaaggca catagataag ggatatacat ccccagcatt
    34141 ttgacaactt catccggatc actctggagc acactaatcg caaatccatc attgaaagtt
    34201 gtaccaaagg tacaccatga atagaggcta atactcatgt ctgccgcagt cagtaacaca
    34261 aatggtgcgg caacaagcag gcgaagcgga agggatgata tacgttttac aaccaggatg
    34321 gtcaggacga agaacagcaa tcgctgaacc atatgcgcgc cggaagaggc gactaagttc
    34381 gtaataccaa aaagaataac acaaagatac aacaaccaat gagatttaaa acttctagca
    34441 aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa caattatcaa
    34501 caatgttcaa ttttatatca gacatgtttt tcaactctga tccatatcag accgaaacac
    34561 tgttagtttt ttctttttaa atgatgcggc cattgcgttc tcagtaatta aataacatta
    34621 tcaatgcgtc tctaaacatt acgcagaacg tagccatcag tagttatact gacaggagca
    34681 ggatggttta tcgagaagta aacatatccg tattcgtacc ggaactggag ttgacacaat
    34741 aaagtgccaa ttatgtcagc agaagggaaa agtttataac taaggcgtac cggcaccatc
    34801 gtttcaaggt accagctacg agtaaagcaa ctggacgaga tacagatacc tgacaaccat
    34861 tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt ctggaatttg
    34921 gtaccgagga cgggacttga acccgtaagc cctattgggc actaccacct caaggtagcg
    34981 tgtctaccaa ttccaccacc tcggcacgga tactactttt tagttcggga tatcgctggt
    35041 cggcttagcc ggagcagctg gttgagtttg ttcggttttc accggtgcac tcagattttc
    35101 ccattcgcta cctttattgg ttttgttgct attgatgtta cccagcacca gactgatgat
    35161 gaagaataac gttgccagca gcgccgtcat gcgggtcatg aagttaccag aaccacttga
    35221 accaaacagc gtagcggaag cgcctgctcc gaaggaggct cccatatcag cgcctttacc
    35281 ttgctgcagc atgatcagac caacaaggcc aattgccaca ataaggaaaa ctactaaaag
    35341 agcttcgtac ataatcaacc tgttccttgc ggagttgccg cgtaccaatg cttcaaccaa
    35401 taaagcggga gtttttaatc tttcccactg aagcgggtgt gaatactaac caaagcgaat
    35461 gaccttcgca agggcaattt tatcgcattg tatcaactgc ggaaaaaaac agcaaaaccg
    35521 atgtgttcgc tgagaaaaaa ggcgacaatc gccgcctttt tagccagtta tctaacgctt
    35581 taaacggctt ttactgcatc ggcgatgcgg tgtgcaaatt cagtcacctg cgcttcgtct
    35641 tcgccttcca ccatcacgcg aattaacggt tcggtgccgg atttacgcag caacacgcgt
    35701 ccacggttgc ccagcgcagc ttcaacctct gcggtcacgg ctttaactga ctcatgctca
    35761 agtggatcgc cgctacctgc ggtgtaacgt acgttaacca gaatctgcgg gaacattttc
    35821 atgccgctgc aaaggtcgtg caggctcata tggttacgtg ccatcgccgc cagcacctgc
    35881 aagccagcaa cgatgccgtc accggtagta gttttatcca gcaggatcac atgaccggaa
    35941 ttctctgcac cgatacgcca gcctttctcc tgcatttttt ccagtacgta gcggtcaccc
    36001 actttcgcgc gcgcaaatgg aattcctaac tgtttcagcg ccagttcaag ccccatgttg
    36061 ctcatcaatg tacccacagc gccaccacgc agctggccct gacgaagacc ttcacgcgcg
    36121 atgatataca tgatctgatc gccatcgact ttattgcctt catggtcaac cataatcacg
    36181 cgatcgccat cgccgtcgaa ggcaataccg agatccgctt tttcagccag cacacgagcc
    36241 tggagcgcgc gaacgtcggt agcccccact tcggcattga tgtttacacc gtttggctca
    36301 caaccgatag cgataacgtt cgcccccagt tcgcgcagca cgttcggcgc gatgtgataa
    36361 gtcgcaccgt ttgcacaatc caccacaatc ttcagttcac tgaggctaag ttcgttcggg
    36421 aacgtggctt tgcaaaactc gatatagcga cccgcggcat caacgatacg gctggcttta
    36481 cccagttctg ccgaatcaac gcagctgatc tccttttcca tttccgcttc gatggcctct
    36541 tctaccgcat ccggcagttt ggtgccgtcg atagagaaga atttaatgcc attatcgtag
    36601 aacgggttat gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt acgcgtcaga
    36661 taagccacgg ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc cgctgccgcc
    36721 agacccgctt ccagtgctga ctccagcata tagccagaaa tacgcgtgtc tttaccaata
    36781 ataatcttac gggagccgtg gcgcgccagc actttacccg cggcccaacc cagcttaagc
    36841 acaaaatcag gtgtgatcgg cgcatcccct acacgaccac gaatcccatc ggtaccgaaa
    36901 tatttacgat tactcatagc gttagttttc ctttgcagac agagtggctt ccaccacccg
    36961 catcgcttct acggtttctt tgacgtcatg aacacgaatg atgtgcgcgc cttgcattgc
    37021 ggcaatgacc gcacaggcca gactaccgct caggcgctcg gacggcccca cgttcagcag
    37081 ctgcccaatc atcgattttc gtgacatacc caccaacagc ggcaggttga aatggtgaaa
    37141 ttcagccagg cgcgccagta atgaatagtt atgggagaga tttttaccga aaccgaatcc
    37201 ggggtcgagc aacaatttct cttttgcgat acccgcctgc tcgcaacgtg ctatttgctc
    37261 aataaagtag cgattcactt ctgcaaagac atcgtcatac ttcggagctt cctgcatggt
    37321 ttttggattt ccctgcatat gcatcagaca aaccggtaaa ccggtttctg cagccgcctc
    37381 cagagcgcca ggttcggaaa gggagcggat atcattaata atgtgagcgc caactttcgc
    37441 tgactcacgg atgacttctg gtttggatgt atcgaccgag atccagactt cgaagcgttg
    37501 agcaattgcc tcaaccacag gaataacacg ttgcaactct tcttcaacgc taacttccgc
    37561 cgcccctggg cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag cattgatcat
    37621 cagattcgca tgtttcaccg catctatcag cgagttatgc gtgccaccat ccgaaaagga
    37681 atcaggcgtg acgttgagga tccccattac gtgaggatgg ctaaggtcca gtgaagtacc
    37741 ctgggcaaag agtttcatga tgttatccct ggtatgaatt gataagaaaa aaccccggag
    37801 cacgccccgg ggttttcggt acaaatacag tcatctgatg cgggaactta cttgtcgcct
    37861 aactgctctg acatggtgtt acccgggttc ggcgtacgcg gttcatcaac cggacgagga
    37921 gcctttggac taccattgtc gccagaattg ttagaagcgc ctggttcttc ccagcccgct
    37981 ggcggacgta catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc gatagtctca
    38041 tatttcatga gagcatcttt catcgcatgc agaatatcca tattgtcggt cagaagctga
    38101 cgcgcacgat tatagttacg ctcaatcagt gctttcactt cctggtcgat gatacgtgca
    38161 gtttcatcgg acatatgttt cgctttcgct acgctacggc cgaggaatac ttcaccttct
    38221 tcttccgcgt acagcagtgg acccaatttc tcagagaagc cccactgggt caccatgtta
    38281 cgtgccaggt tggtcgcaac tttaatatcg ttggacgcac cggtagaaac atgttccggc
    38341 ccgtagatga tctcttctgc cagacgacca ccgtacagcg tagaaatctg gctttccagt
    38401 ttctgacggc tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac acccagcgca
    38461 cgaccgcgtg ggataatcgt cactttgtgc accggatcgt gttccggcac caggcgaccg
    38521 ataatcgcat gacccgcttc gtggtaagcc gttgattctt tctgcgcttc cgtcatcacc
    38581 atggagcgac gttccgcacc catcatgatt ttgtctttcg ctttctcgaa ctcaaccatc
    38641 gacacaacgc gtttgttgcc acgagcagcg aacagtgccg cttcgttcac caggttcgcc
    38701 aggtcagcac cggagaaacc aggagtacca cgggcaatga ttgccgcgtc gatatcgggg
    38761 gccaatggta cgcgacgcat gtgaactttc aggatctgct cacgaccgcg aacatctggc
    38821 aagccgacca caacctgacg gtcgaaacgg ccaggacgca gcagggccgg gtcgagaacg
    38881 tccggacggt tagtcgcggc gataacgatg ataccttcgt taccttcgaa gccatccatc
    38941 tcaaccagca tctggttcag agtctgttca cgttcatcgt gaccaccgcc cagaccagcg
    39001 ccacgctggc ggcctacggc gtcgatttca tcgataaaga tgatgcacgg tgccgctttc
    39061 ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca caccgacgaa catttctacg
    39121 aagtcagaac cggagatagt aaagaacgga actttcgctt cgcctgcaat cgctttcgcc
    39181 agcaacgttt taccggtacc cggaggaccg accatcagga cgcctttcgg gatcttaccg
    39241 ccgagtttct ggaagcggct cggctcgcgc agatactcta ccagttcagc aacttcttct
    39301 tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc ttccgtcagc
    39361 atgcgcgctt tgctcttacc aaacgacatg gcacctttgc caccgccgcc ctgcatttga
    39421 cgcatgaaga agatccagac accaatcagc aacagcatcg ggaaccagga gatgaagata
    39481 gaagccagca ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt cttggtcaac
    39541 aggttatcca gtaatttcgg atcctgaacc ggaatgtaag tggtataacg gttactatct
    39601 ttcttggtaa cgttgatttc acgtccgttg atacgcgctt cacgaacctg gtcgttattg
    39661 acctcttgta ggaaggtaga gtaatccacc ttacggccat tagactcgct gggcccaaag
    39721 ctctggaata ctgacatcag cacaacggca atgaccagcc agagtattag gtttttcgcc
    39781 atgtcactca agggattaac ctcttattac aactgtgtta aaaacagcgt caggatactc
    39841 tatatccagc atctttcaaa ctttcgtctg aaatctcccg gttagggttt acgcccggtc
    39901 gctacaatat acacttcccg cgaacgtgca cgagaagagt ccggcttacg aactttgacc
    39961 ttcgtaaaca gggagcgaat ttcccttaga tactcatcga aaccttcgcc ctggaacacc
    40021 ttcactacaa aactgccacc tggcgctaat acatcacgac acatttctag cgccagttcc
    40081 accagataca tggcacgggg gatatccacc gccggtgttc cgctcatgtt tggtgccata
    40141 tcggacatga caacctggac tttgctgtcg ccaacgcgct ccagcagtgc tttcatcacc
    40201 agttcatcac gaaaatcgcc ctgaagaaag tccacaccaa cgataggatc cataggtaaa
    40261 agatcgcaag cgatgatgcg gcctttgccg ccaatttggg tgaccacata ttgtgaccaa
    40321 ccacccggag cagcaccaag gtcgacaacc gtcattcccg gtttaaagag tttgtcactt
    40381 tgctgtattt catcaagttt aaaccaggca cgggaacgta accccttttt ctgtgcctgt
    40441 tgaacatatt tatcgctaaa gtgttcctga agccagcggc tggagctggc agaacgcttc
    40501 ttacctgtca tttaactttc ccatggggat aactcatcgt aaccaattgc gtaaattttt
    40561 acgcgcctat ttggtgatat atgggagatg gcggtagaat gacccgtttt caatcccaac
    40621 gtaagcaaaa aatacgatga atctgagtac taaacaaaaa cagcacctga aaggtctggc
    40681 acatccgctc aagccagttg ttctgcttgg cagtaatggt ttgaccgaag gggtgctggc
    40741 cgagattgaa caagcgttag agcaccatga actcatcaag gtgaaaatcg ccaccgaaga
    40801 tcgcgaaact aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg cctgtaatgt
    40861 acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta aaatctcgct
    40921 gccacgctaa gattatccta aagttacaca catttgctgt gtaaaacgag gggttttccg
    40981 caggcaggag agcaaaatgc cacgctctgt tcgttgataa aaggccgcat agcggccttt
    41041 ttcctttctt tacaatacat caacatcttg agtattgggt aattcttaca ggtattccac
    41101 cttaattact tcaaattcta cttcgccgcc cggcgttttg atgaccacaa catcatcttc
    41161 ttctttgccg atcaggccac gagcaatagg tgagtttaca gaaatcaggt tttgtttaaa
    41221 gtcagcttcg tcatcgccaa cgatgcgata agtctgttct tcgtcagaat ccagattcag
    41281 caccgttacg gtagcaccaa aaataacgcg cccattgttg ggcattttgg tgacatcaat
    41341 cacctgcgcg ttcgacagct tggcttcgat gtctttgata cggccttcgc agaagccctg
    41401 ctgttcacga gctgcgtggt attcggcgtt ttctttcagg tcgccatgct cacgcgcttc
    41461 cgcgatagca gcaatgattt caggacggcg cacagatttc agaaaatcca gctcttcgcg
    41521 taatttttca gcgccgcgta aggtcatcgg aatagcttgc atttgttata cctcttgaat
    41581 attcctgatg gggcaagtct tcacccaccc cggctgttcg gcctgcccgg cataactcct
    41641 caccgggacc agaagcaaaa aaataccgac ccgggtacaa gtcccaggtc agctacaatt
    41701 cacattttga tagtcatttt accctgaagt tcccgaaggg tcatcgttta ctttataggg
    41761 cgttgcgccg tagtatgacg gctcgattcc aggttgttag cgcgagatta tgcgattttc
    41821 cagatttatc atcggattga ccagctgtat agcgttcagt gttcaggccg cgaatgttga
    41881 tgagtacgtt actcaactcc ccgctggtgc caaccttgcc ctgatggtgc aaaaagtcgg
    41941 cgcgtcggcc cccgctattg attaccacag tcagcagatg gcgctgcccg ccagtacgca
    42001 gaaagtgatt actgcgctgg cggcgttgat tcaactcggc cccgattttc gttttaccac
    42061 gacgcttgaa accaaaggca atgtggaaaa cggcgtactt aagggtgact tagtggcgcg
    42121 atttggtgcc gatccgacgt taaaacgtca ggatattcgc aatatggtcg cgactttgaa
    42181 aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata gatacctcca ttttcgccag
    42241 ccacgataaa gcccccggct ggccatggaa tgacatgaca caatgcttta gcgctccgcc
    42301 tgccgccgcc atagttgacc gcaactgttt ctccgtctcg ctctacagtg cccaaaaacc
    42361 cggtgatatg gcctttatac gcgtggcatc ttattacccc gttacgatgt tcagccaggt
    42421 acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc gaactggatg tggtgccagg
    42481 cgacctgaac cgctttacgc tgacgggatg cctgccacaa cgttctgagc cgctcccgtt
    42541 ggcttttgcc gtacaggatg gagccagcta tgccggggca attctgaaag atgagttaaa
    42601 acaggcgggt atcacctgga gtggaacact gctgcgccag actcaggtta acgaacctgg
    42661 aacggtagtt gccagtaaac agtcggcccc gctgcacgat ctgcttaaga ttatgctgaa
    42721 aaagtcagac aacatgatcg ccgatacggt tttccgcatg ataggccatg cgcgcttcaa
    42781 tgtgcctgga acatggcggg ccgggtcgga cgccgtgcgt cagatcctgc gccagcaagc
    42841 cggtgtcgat attggaaaca ccattattgc cgatggttca gggctttcgc ggcataacct
    42901 gattgccccc gccaccatga tgcaggtgct gcaatacatt gcccaacacg acaatgaact
    42961 taactttatc tccatgctgc cgctggcggg ctatgacggc tctttgcagt accgtgcagg
    43021 tctgcatcag gcgggcgtgg atggcaaagt ctcagcgaaa accggttcgt tgcagggggt
    43081 atataacctg gcgggattca ttaccacagc gagcgggcaa cgaatggcgt ttgtgcaata
    43141 tctttctggc tatgcagtag aaccggcgga tcagcgtaat cgccgtattc cgttagtgcg
    43201 tttcgaaagc cgtttgtata aagatattta ccagaacaat tagtcaaaaa gaaaccccgg
    43261 catatggcc
//
LOCUS       DAPHJE010000027        42780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPHJE010000027 DAPHJE010000000
VERSION     DAPHJE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.027"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2764)
                     /gene="rhsC"
                     /locus_tag="RH222_001474"
     CDS             complement(<1..2764)
                     /gene="rhsC"
                     /locus_tag="RH222_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HDV3796522.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDS
                     LNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRY
                     RYDNPHSDLPCATDDATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHRE
                     EGLSQYHAYDSRGQLTAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISEGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRD
                     YTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPE
                     LHPDSTL"
     gene            complement(2924..3136)
                     /gene="rpmE"
                     /locus_tag="RH222_001475"
     CDS             complement(2924..3136)
                     /gene="rpmE"
                     /locus_tag="RH222_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV3796523.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            3339..5537
                     /gene="priA"
                     /locus_tag="RH222_001476"
     CDS             3339..5537
                     /gene="priA"
                     /locus_tag="RH222_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV3796524.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQVGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            5693..6718
                     /gene="cytR"
                     /locus_tag="RH222_001477"
     CDS             5693..6718
                     /gene="cytR"
                     /locus_tag="RH222_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV3796525.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            6810..7769
                     /gene="ftsN"
                     /locus_tag="RH222_001478"
     CDS             6810..7769
                     /gene="ftsN"
                     /locus_tag="RH222_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV3796526.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            7862..8392
                     /gene="hslV"
                     /locus_tag="RH222_001479"
     CDS             7862..8392
                     /gene="hslV"
                     /locus_tag="RH222_001479"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV3796527.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            8402..9733
                     /gene="hslU"
                     /locus_tag="RH222_001480"
     CDS             8402..9733
                     /gene="hslU"
                     /locus_tag="RH222_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV3796528.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            9800..10726
                     /gene="menA"
                     /locus_tag="RH222_001481"
     CDS             9800..10726
                     /gene="menA"
                     /locus_tag="RH222_001481"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV3796529.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            10819..11304
                     /gene="rraA"
                     /locus_tag="RH222_001482"
     CDS             10819..11304
                     /gene="rraA"
                     /locus_tag="RH222_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV3796530.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(11389..11634)
                     /gene="zapB"
                     /locus_tag="RH222_001483"
     CDS             complement(11389..11634)
                     /gene="zapB"
                     /locus_tag="RH222_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV3796531.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            12060..12905
                     /gene="glpF"
                     /locus_tag="RH222_001484"
     CDS             12060..12905
                     /gene="glpF"
                     /locus_tag="RH222_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV3796532.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            12928..14436
                     /gene="glpK"
                     /locus_tag="RH222_001485"
     CDS             12928..14436
                     /gene="glpK"
                     /locus_tag="RH222_001485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV3796533.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            14571..15581
                     /gene="glpX"
                     /locus_tag="RH222_001486"
     CDS             14571..15581
                     /gene="glpX"
                     /locus_tag="RH222_001486"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV3796534.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            15678..16424
                     /gene="fpr"
                     /locus_tag="RH222_001487"
     CDS             15678..16424
                     /gene="fpr"
                     /locus_tag="RH222_001487"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV3796535.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(16429..16857)
                     /gene="uspD"
                     /locus_tag="RH222_001488"
     CDS             complement(16429..16857)
                     /gene="uspD"
                     /locus_tag="RH222_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV3796536.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(16884..17183)
                     /locus_tag="RH222_001489"
     CDS             complement(16884..17183)
                     /locus_tag="RH222_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV3796537.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(17395..17835)
                     /locus_tag="RH222_001490"
     CDS             complement(17395..17835)
                     /locus_tag="RH222_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3796538.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            17936..18535
                     /locus_tag="RH222_001491"
     CDS             17936..18535
                     /locus_tag="RH222_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV3796539.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            18643..19410
                     /gene="tpiA"
                     /locus_tag="RH222_001492"
     CDS             18643..19410
                     /gene="tpiA"
                     /locus_tag="RH222_001492"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV3796540.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(19465..20220)
                     /gene="cdh"
                     /locus_tag="RH222_001493"
     CDS             complement(19465..20220)
                     /gene="cdh"
                     /locus_tag="RH222_001493"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV3796541.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVRKQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(20327..21316)
                     /gene="sbp"
                     /locus_tag="RH222_001494"
     CDS             complement(20327..21316)
                     /gene="sbp"
                     /locus_tag="RH222_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV3796542.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(21635..22597)
                     /gene="pfkA"
                     /locus_tag="RH222_001495"
     CDS             complement(21635..22597)
                     /gene="pfkA"
                     /locus_tag="RH222_001495"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV3796543.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(22778..23680)
                     /gene="fieF"
                     /locus_tag="RH222_001496"
     CDS             complement(22778..23680)
                     /gene="fieF"
                     /locus_tag="RH222_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV3796544.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(23829..24329)
                     /gene="cpxP"
                     /locus_tag="RH222_001497"
     CDS             complement(23829..24329)
                     /gene="cpxP"
                     /locus_tag="RH222_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV3796545.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            24479..25177
                     /gene="cpxR"
                     /locus_tag="RH222_001498"
     CDS             24479..25177
                     /gene="cpxR"
                     /locus_tag="RH222_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV3796546.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            25174..26547
                     /gene="cpxA"
                     /locus_tag="RH222_001499"
     CDS             25174..26547
                     /gene="cpxA"
                     /locus_tag="RH222_001499"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV3796547.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(26653..27327)
                     /gene="yiiM"
                     /locus_tag="RH222_001500"
     CDS             complement(26653..27327)
                     /gene="yiiM"
                     /locus_tag="RH222_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV3796548.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(27476..28459)
                     /gene="kdgT"
                     /locus_tag="RH222_001501"
     CDS             complement(27476..28459)
                     /gene="kdgT"
                     /locus_tag="RH222_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV3796549.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPIITSIWSRKVKARAAKIEILGTVK"
     gene            complement(28719..29339)
                     /gene="sodA"
                     /locus_tag="RH222_001502"
     CDS             complement(28719..29339)
                     /gene="sodA"
                     /locus_tag="RH222_001502"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV3796550.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            29624..30658
                     /gene="rhaT"
                     /locus_tag="RH222_001503"
     CDS             29624..30658
                     /gene="rhaT"
                     /locus_tag="RH222_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDV3796551.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(30655..31521)
                     /gene="rhaR"
                     /locus_tag="RH222_001504"
     CDS             complement(30655..31521)
                     /gene="rhaR"
                     /locus_tag="RH222_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDV3796552.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQTVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(31577..32413)
                     /gene="rhaS"
                     /locus_tag="RH222_001505"
     CDS             complement(31577..32413)
                     /gene="rhaS"
                     /locus_tag="RH222_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDV3796553.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            32701..34170
                     /gene="rhaB"
                     /locus_tag="RH222_001506"
     CDS             32701..34170
                     /gene="rhaB"
                     /locus_tag="RH222_001506"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV3796554.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            34167..35426
                     /gene="rhaA"
                     /locus_tag="RH222_001507"
     CDS             34167..35426
                     /gene="rhaA"
                     /locus_tag="RH222_001507"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV3796555.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            35509..36333
                     /gene="rhaD"
                     /locus_tag="RH222_001508"
     CDS             35509..36333
                     /gene="rhaD"
                     /locus_tag="RH222_001508"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV3796556.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            36343..36657
                     /gene="rhaM"
                     /locus_tag="RH222_001509"
     CDS             36343..36657
                     /gene="rhaM"
                     /locus_tag="RH222_001509"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV3796557.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            36958..37404
                     /locus_tag="RH222_001510"
     CDS             36958..37404
                     /locus_tag="RH222_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV3796558.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            37415..38866
                     /locus_tag="RH222_001511"
     CDS             37415..38866
                     /locus_tag="RH222_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV3796559.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTLVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            38856..39926
                     /locus_tag="RH222_001512"
     CDS             38856..39926
                     /locus_tag="RH222_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3796560.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            39926..41674
                     /locus_tag="RH222_001513"
     CDS             39926..41674
                     /locus_tag="RH222_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV3796561.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNSVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNCITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(41724..42779)
                     /locus_tag="RH222_001514"
     CDS             complement(41724..42779)
                     /locus_tag="RH222_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV3796562.1"
                     /translation="MKRNLLSSAIIVAIMSLGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNSLR"
BASE COUNT        10158 a        11221 c        11210 g        10191 t
ORIGIN      
        1 tgagggtgct gtccgggtgc agctccgggt cgggcaggcg gttacctgcc gggtctgtgg
       61 catacgggat gcggatatca aggttcgctg cggtggtgtg aacgccggtc agcctgccgg
      121 tggtgctgta gctgtaactc cgggtctggc gcgggctgct gatgcggatg agttcgccgt
      181 tgtcgttcca ggtgtaatcg cggtcagaca gcaggctgtt caggtgctgg ctctgtaact
      241 gcccggcagg ggtgtaagcg gtggtgagtt cataacgacc gaagctgcgc agcgtttccc
      301 ggtgcaggcg gtcgcgggtg tactccacca gcggtgtgtc gccgagtttc atgcctgcca
      361 ggtaaccgct gccgtaggtc agccattcca cggcgggcag gctgtccggt atacagcggt
      421 tcgccagccc ctgcgcgttg tacgcatgtc tggtctcatg ctgccagagc agtgcttccg
      481 tctgcgggtg atgcaccgtc tgacgctcac cggtcagccg gcctttctca tcatacccgt
      541 aatgcaccgt cacccggtgc ccttcgctga tatggctgat gtctgtcagc cagccgcgtt
      601 cgtcatactg ccactgctct gcggtttcgc cattcacggt gcggtgcgtg aggcggtctg
      661 cttcgtcata gtgccagtgg gtgaccagcc cctcatcctc gctgcggata agtttgccgg
      721 tcaggtcgtg gtgataacgc tgtgtgcggc cgtcaaagcc ggtttcctgt atcagccggt
      781 cgagtacatc gtaacggaag gtggtgtggc tgccgttttc actggtcagg cggatgaccc
      841 gtccggcagc atcgtattcc atactgcgcg tcagaccgcc ctgcgtggta cagatggctt
      901 ttccccacgc atcgtactgt gtcccgtttc tgctgccgtc cggggcaatg acggtggtca
      961 ggtcaccggc ggcgttgtat tcataccgcg tttcatggcc ctgcgtgtct ttcaccgcag
     1021 ttaactgtcc acggctgtcg tatgcgtggt actggctcag gccttcctcg cggtgcaccg
     1081 ccgtcacctg accaaaacgg tcatggtcat agcgggttac ataaccggaa cagtcagtga
     1141 aggtcagcaa ctgaccgtaa cggctccacg tcatggtttt ccggctgccg gtggcatcgt
     1201 ccgttgcgca ggggaggtca ctgtgtggat tatcataacg gtagcgggtg atatcgccgt
     1261 caggggcggt ttcctgaatc agacgtccgt actcatcata tttgcggcgc atttccagcc
     1321 cgtcaggccc ggtggctgac gttaactggc tgtggtggtt atagtaaaac gccgatgccc
     1381 tgccatccgg cgtggtgatg cgcgtgatga gtcccgtcac cacatccggg ctgtactctg
     1441 ttgtcctgcc tgcggcatcc gtctgtgcct taagcctgcc caccgcatca aactgactct
     1501 gcgtgacgct gccgtccgcg tgttcctttt tcaccacccg cttcagcccg gcttcaccct
     1561 gcgtgtgcag cacttcacgg cggttcaggc tgtcggtgat ggtgatgcgg tccttctcat
     1621 actgatacgt gtagcttaag cctgccgggt ttagctgttc tgtcacccgc ccgtcgctgt
     1681 cgtaacggta acggatttcc ggccggcccg tgtgacggtg cgccaccatc cggccccggt
     1741 atttatcatc gtaagtaaag ctgcgcacct gtgtattgct gcggtcatac accgccgcca
     1801 gttcgccgcg tggcgtccag ccatagcgca ccagcggcgc ggcaggtaaa ttctccgggt
     1861 attccgggtc gtgcgtcagc cacacggcag acagacggat gccgttgtcc cggccatatt
     1921 cggtgtaacc cggcagggta tcaggaaaag cggacggttc cgtcccgccg gaaatggcct
     1981 gctgccgggc ttcttctgcc cgctgcgcct gcgtggtcag taccagccgg aagtgacgcc
     2041 cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc accggcggct tcgcggtgga
     2101 acgtctgtgt gcgcccgaag cggtccacca gcccggtcag tacccggtac ggcggcagcg
     2161 gcgcaggcag cacctcatcc gcttccggca cccgctcaca ccagccgagc agccaccacg
     2221 gcccctgcgg actgtttgtc gccagataac gatgcggact taagcggagt tcttccggca
     2281 gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccacg ccgccgcgca
     2341 ccagccacag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg tgctcaaaat
     2401 acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc tgtaagcgga
     2461 tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc gtttttgtcc
     2521 ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg gcgatgtcgg
     2581 tttcaccggg aaggaccttt gcaccgagca gcggattgac cggatggccg gacgtcaccc
     2641 cgccggggca caccgaacag gccacgccgg tgggggcacc gatgcgcacc ccggctgaac
     2701 cctggacaat gctaccgcca tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc
     2761 tcatcgtaaa atccttttga aataaataag aaccagaagc ctagcgccag tcaacaacag
     2821 gaaatgataa tgagttaaca ccccggaatt tttcagaatt tataaaaggc aaattacagg
     2881 cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg ctgcccggga
     2941 tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca cgctgtttgc
     3001 cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg
     3061 agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg tatttcgggt
     3121 gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc cagccctaac
     3181 gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt acgaaggcgc
     3241 gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct acggcaatgt
     3301 gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc cacgttgcct
     3361 tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg acggttaaag
     3421 ctgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg attgtggtat
     3481 cagttagcga tgccagcgaa ctgccgctca atgagctaaa agcggtagtc gaagtgctgg
     3541 atagtgagcc ggtgtttact cactccgtct ggcgattgct gctatgggcg gcagattact
     3601 atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttacta cgccaggggc
     3661 ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggt caggcggtgg
     3721 atctgaacag cctgaaacgc tcacccaagc aacaacaggc gctggcagcg ttacggcaag
     3781 gcaaaatctg gcgcgaccag gttgccacgc tcgaatttaa tgatgccgcg ttgcaggcgc
     3841 tgcgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc gactggcgaa
     3901 cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaggcc accgccgttg
     3961 gcgcaattca tagtgcggcg gataccttct ctgcctggct gctggcgggc gttaccggtt
     4021 ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg
     4081 cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt tttcgtgaac
     4141 gttttaatgc ccccgtagaa gttctgcatt ccggcttgaa cgacagcgag cgtctttcgg
     4201 cgtggttgaa agcgaaaaat ggtgaggcgg cgattgtgat cggcacccgc tccgcgctgt
     4261 ttacgccgtt taaaaatctc ggtgtgattg tcattgatga agagcacgac agttcctaca
     4321 agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtttatcgt gcgcacagcg
     4381 agcaaatccc gattattctt ggctccgcaa cacccgcact ggaaacgtta tgcaacgtcc
     4441 agcagaaaaa ataccgcctg ctgcgcctga cgcgtcgggc ggggaatgcg cgtccggcaa
     4501 ttcaacacgt gctggattta aaaggtcaga aggtgcaggt ggggctggct ccggcgttaa
     4561 tcacccgtat gcgccagcat ttacaggccg acaaccaggt tatcctcttt cttaaccgcc
     4621 gtggctttgc gcctgccttg ctgtgccacg actgcggctg gattgccgaa tgtccacgtt
     4681 gcgatcacta ctacacgctg catcaggcgc aacagcattt gcgctgtcac cactgtgaca
     4741 gccaacgccc ggtgccgcgc cagtgccctt cctgcggttc cacgcaccta gtacccgtgg
     4801 ggctgggcac cgaacagctt gaacagacgc ttgcgccgat gttccccggc gtgcccattt
     4861 cacgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa ctggcggaag
     4921 tgcatcgcgg cggcgcgagg atcttgattg gcacgcaaat gctggcgaaa ggtcaccact
     4981 ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt tctgccgatt
     5041 ttcgctcggc agaacgtttc gcccagcttt acacccaggt cgccggtcgt gccgggcgtg
     5101 cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa
     5161 cgttgctcta taaaggctac gacgcctttg ccgaacaggc gctggctgag cgacgcatga
     5221 tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc
     5281 atgcgccgct gttcctgcaa caactgcgta atctgatcct ttccagcccg ctggcagacg
     5341 acaaactgtg ggttctcggc ccggttccgg ctctggcacc taagcgtggt ggccgctggc
     5401 gctggcagat cttgttgcag cacccttccc gcgtgcgctt gcaacacatc attagtggta
     5461 cgctggcgct tattaataca ataccggatt cccgcaaggt gaaatgggtg ttggatgtcg
     5521 atcccatcga aggctaatgc gcaaacgttg cgaggcggat cgaaaaattc aatattcatc
     5581 acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga
     5641 tgtgatgtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc
     5701 gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac
     5761 agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca cccgtaatcg
     5821 ggttgaaaaa gcggcccggg aagtgggtta tttaccgcaa cctatggggc gcaacgtcaa
     5881 gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc ccttctttag
     5941 cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg tgctgattgg
     6001 cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca tcaccaagca
     6061 aattgatggc atgttgctgc tgggctcaag gctgccgttt gatgccagca ttgaggaaca
     6121 gcgtaatctg ccgccgatgg tgatggcgaa cgaatttgca ccggaactgg agctgcctac
     6181 cgttcatatc gacaatctga ccgccgcatt tgatgcagta aattatttat atgagcaagg
     6241 gcataaacgg attggctgta tagccggtcc cgaagagatg ccactgtgtc actaccgcct
     6301 gcaaggctat gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc aatacatcgc
     6361 ccgtggcgac ttcaccttcg aagccggaag caaagcgatg cagcagctgc ttgatcttcc
     6421 acaaccgcct actgctgtct tctgccatag cgatgtgatg gcgctcggcg cactttctca
     6481 ggcaaaacgc caggggctga aagtcccgga agacctttcc ataatcggtt ttgataacat
     6541 cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc gttacgaaat
     6601 cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg ttggcagtgg
     6661 ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt taccttaaag
     6721 taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac atggcgggct
     6781 gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta cgccgcagcc
     6841 aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga aatctgcctg
     6901 cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt atcggtggtc
     6961 tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc cagaaagtga
     7021 ccggaaacgg actaccacca aaaccagaag aacgctggcg ctacatcaaa gagctggaaa
     7081 gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc tggtggtgaa gtgaaaacgc
     7141 cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc
     7201 agcagccaac gcagctggtt gaggttccgt ggaatgaaca gacgccggaa cagcgtcagc
     7261 aaacgctaca gcgccaacgt caggcgcagc aattagcgga acagcaacgc ctggctcagc
     7321 agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtctcaa gccgcgccgg
     7381 tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac caggatctgc
     7441 tgcaaacccc tgcgcacaca accgcgcagt cgaagccgca gcaagctgcg ccggttactc
     7501 gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc
     7561 agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag ctggcgttcg
     7621 aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc attggcccgg
     7681 tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa
     7741 actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat ctataattgc
     7801 attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa ggggtcagct
     7861 cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca
     7921 ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa
     7981 cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc tgttcgaact
     8041 gtttgaacgt aaactggaaa tgcatcaggg ccatctggtt aaagccgccg ttgagctggc
     8101 aaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga
     8161 tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct
     8221 tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa
     8281 cactgaactt agcgcccgtg aaattgctga aaaggcgttg gatattgcag gcgacatttg
     8341 catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt aaggatctcc
     8401 catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca tcatcggcca
     8461 ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct
     8521 caacgaagag ctgcgccatg aagtgacccc gaaaaatatt ctgatgatcg gcccgaccgg
     8581 tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa
     8641 agttgaagcg accaaattca ccgaagtggg ctacgtcggt aaggaagtgg attctattat
     8701 tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg
     8761 ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc cacctgctaa
     8821 aaacaactgg ggacagaccg aacagcagca ggaaccgtcc gctgctcgtc aggcattccg
     8881 caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc ttgccgcagc
     8941 accgatgggc gttgaaatta tggctcctcc aggcatggaa gagatgacca gccagctgca
     9001 gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga
     9061 cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa
     9121 gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa
     9181 aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga
     9241 cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga
     9301 ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg acctaatccc
     9361 ggaactgcaa ggtcgtctgc caatccgcgt tgaactgcag gcactgacca ccagcgactt
     9421 cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag cactgatggc
     9481 gactgaaggc gtaaatatcg agtttaccga ctccggtatt aaacgcatcg cggaagcggc
     9541 atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga
     9601 gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa atatcactat
     9661 tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag atctgagccg
     9721 ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct gaaaggcccc
     9781 atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg cgtggctgga
     9841 aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg ggacggcgct
     9901 ggcatggtgg caaggtcact tcgatccgct ggtcgccctg ctggcactaa ttaccgccgg
     9961 gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa aaggcagcga
    10021 taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga
    10081 gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc tggcactggt
    10141 tgcagtggca tgccatacgc tggccgattt tgtcggtttc ctgattcttg gcgggttgtc
    10201 gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata tcggtctggg
    10261 tgatatttcc gtactggttt tctttggctg gttgagcgtc atggggagct ggtatttaca
    10321 ggctcataca ttgattccgg cactgatcct tccggcgacc gcctgtggcc tgctggcaac
    10381 ggcagtactg aacattaata acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa
    10441 cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat
    10501 gggctctctg gtatgtctgg cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg
    10561 gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga tgcgggaaat
    10621 ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt tactgactaa
    10681 cctgctgttt gttttaggga tattcctaag ccagtgggca gcataactga caaatatcaa
    10741 ttaacaattg atgattttgc caacagccca catagcgcga tatactgaaa attctcgcag
    10801 caactgaatg ttaagcctat gaaatacgat acttccgagc tttgtgacat ctatcaagaa
    10861 gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc gtttggcgga
    10921 caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct gctcgaacag
    10981 aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc
    11041 gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt tatttacggc
    11101 gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc gatggcggca
    11161 attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc
    11221 ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat tattctttca
    11281 gaagatccgc tggatattga atgataagaa aggcaccgca aggtgccttt tttctgcgtt
    11341 acctgttggc ctacacagta aagaaattac gcgaaagatg aagcgtaatc agacctcttc
    11401 catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt ctttcagatg
    11461 gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt cctgcgacag
    11521 tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga tggtatcaat
    11581 cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg tcatacctct
    11641 cctgaattgc aaggcgttga tggataaaaa accctcgtcc cgattaccgg tgacgcctta
    11701 ataaatacga gcgcacttta gttagctccg attgtatgaa gccgcgccat cgctgtccag
    11761 cggcacgcct tgcagattac ggtttgccac acttttcatc cttctcctgg agacataatc
    11821 cacaccaatc gaaaatgtta ataaatttgt tgcgcgaatg atctaacaaa catgcatcat
    11881 gtacaatcag atggaataaa tggcgcgata acgctcattt tatgacgaag cacacacatt
    11941 ttaagttcga tatttctcgt ttttgctcgt taacgataag tttacagcat gcctacaagc
    12001 atcgtggagg tccgtgactt tcacgcatac aacaaacatt aactcttcag gatccgatta
    12061 tgagtcaaac atcaaccttg aaaggccagt gcattgctga attcctcggt accgggttgt
    12121 tgattttttt cggtgtgggt tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc
    12181 agtgggaaat cagtgtcatt tggggactgg gggtggcaat ggctatctac ctgaccgcag
    12241 gggtttccgg cgcgcatctt aatcccgctg ttaccattgc attgtggctg tttgcctgtt
    12301 tcgacaagcg caaagttatt ccttttatcg tttcacaagt tgccggcgct ttctgcgctg
    12361 cggctttagt ttacgggctt tactacaatt tatttttcga cttcgagcag actcatcaca
    12421 ttgttcgcgg cagcgttgaa agtgttgatc tggctggcac tttctctact taccctaatc
    12481 ctcatatcaa ttttgtgcag gctttcgcag ttgagatggt gattaccgct attctgatgg
    12541 ggctgatcct ggcgttaacg gacgatggca acggtgtacc acgcggccct ttggctccct
    12601 tgctgattgg tctactgatt gcggtcattg gcgcatctat gggcccattg acgggttttg
    12661 ccatgaaccc agcgcgtgac tttggtccga aagtctttgc ctggctggcg ggctggggta
    12721 atgtcgcctt taccggcggc agagacattc cttacttcct ggtgccgctt tttggcccta
    12781 tcgttggcgc gattgtaggt gcatttgcct accgcaaact gattggtcgc catttgcctt
    12841 gcgatatctg tgttgtggaa gaaaaggaaa ccacaactcc ttcagaacaa aaagcttcgc
    12901 tgtaatatga ctacgggaca attaaacatg actgaaaaaa aatatatcgt tgcgctcgac
    12961 cagggcacca ccagctcccg cgcggtcgta atggatcacg atgccaatat cattagcgtg
    13021 tcgcagcgcg aatttgagca aatctaccca aaaccaggtt gggtagaaca cgacccaatg
    13081 gaaatctggg ccacccaaag ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt
    13141 tccgatcaaa ttgcagctat cggtattacg aaccagcgtg aaaccactat tgtctgggaa
    13201 aaagaaaccg gcaagcctat ctataacgcc attgtctggc agtgccgtcg taccgcagaa
    13261 atctgcgagc atttaaaacg tgacggttta gaagattata tccgcagcaa taccggtctg
    13321 gtgattgacc cgtacttttc tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc
    13381 tctcgcgagc gtgcacgtcg tggtgaattg ctgtttggta cggttgatac gtggcttatc
    13441 tggaaaatga ctcagggccg tgtccatgtg accgattaca ccaacgcctc tcgtaccatg
    13501 ttgttcaaca tccataccct ggactgggac gacaaaatgc tggaagtgct ggatattccg
    13561 cgcgagatgc tgccagaagt gcgtcgttct tccgaagtat acggtcagac taacattggc
    13621 ggcaaaggcg gcacgcgtat tccaatctcc gggatcgccg gtgaccagca ggccgcgctg
    13681 tttggtcagt tgtgcgtgaa agaagggatg gcgaagaaca cctatggcac tggctgcttt
    13741 atgctgatga acactggcga gaaagcggtg aaatcagaaa acggcctgct gaccaccatc
    13801 gcctgcggcc cgactggcga agtgaactat gcgttggaag gtgcggtgtt tatggcaggc
    13861 gcatccattc agtggctgcg cgatgagatg aagttgatta acgacgccta cgattccgaa
    13921 tatttcgcca ccaaagtgca aaacaccaat ggtgtgtatg tggttccggc atttaccggg
    13981 ctgggtgcgc cgtactggga cccgtatgcg cgcggggcga ttttcggtct gactcgtggg
    14041 gtgaacgcta accacattat acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac
    14101 gtgctggaag cgatgcaggc cgactctggt atccgtctgc acgccctgcg cgtggatggt
    14161 ggcgcagtag caaacaattt cctgatgcag ttccagtccg atattctcgg cacccgcgtt
    14221 gagcgcccgg aagtgcgcga agtcaccgca ttgggtgcgg cctatctcgc aggcctggcg
    14281 gttggcttct ggcagaacct cgacgagctg caagagaaag cggtgattga gcgcgagttc
    14341 cgtccaggca tcgaaaccac tgagcgtaat taccgttacg caggctggaa aaaagcggtt
    14401 aaacgcgcga tggcgtggga agaacacgac gaataatgta aatgccgaat gaagcgttta
    14461 tgccgcatcc ggtagtcccg aaacgtgcgg gggcaacccc gcacacatca ataatccctc
    14521 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    14581 aacttgccat cgaattttcc cgcgtcaccg aatcagcggc gctggctggc tacaaatggt
    14641 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    14701 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    14761 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    14821 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    14881 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    14941 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    15001 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    15061 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    15121 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    15181 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    15241 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    15301 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    15361 gtaaagtatt gcgcctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    15421 gtattaccaa aggcgatctg ctggaaggca ttagccgcaa aggcaatatc gcgactaccg
    15481 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    15541 tggatcgcaa agacccggaa atgcaggtgc acatcctctg attgatttga tcgattgagc
    15601 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagttaat
    15661 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    15721 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    15781 aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    15841 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    15901 taagcccacg attggcggca ctgaaaccag gcgatgaagt gcaggtggtt agcgaagcag
    15961 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    16021 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    16081 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    16141 tgcaagaact ggaaaaacgc tacgaaggaa aactgcgcat tcagacggtg gtcagtcggg
    16201 aaacggcagc ggggtcgctc accgggcgga taccggcgtt aattgaaagt ggggaactgg
    16261 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    16321 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt
    16381 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    16441 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    16501 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    16561 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    16621 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    16681 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctca agccatcatc
    16741 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccagag ctttattcac
    16801 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    16861 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    16921 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    16981 ttaatcttgt aggccacgct ggccccttca tctgcagagg atgcagcggc ggcaagctct
    17041 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    17101 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    17161 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    17221 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    17281 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    17341 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggttaatt
    17401 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    17461 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    17521 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgcccagt tacccgccag
    17581 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    17641 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    17701 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    17761 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    17821 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    17881 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    17941 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    18001 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    18061 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccta gcctgccact
    18121 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    18181 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    18241 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    18301 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    18361 aagccagaaa aaactgcagt cgctgctaac ggcggggaaa aacaaacgtt attacaccga
    18421 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    18481 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    18541 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    18601 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    18661 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    18721 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    18781 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    18841 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    18901 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    18961 gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg gcctgactcc ggttctgtgc
    19021 atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg aagaagtttg cgcacgtcag
    19081 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttac
    19141 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    19201 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    19261 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagat
    19321 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    19381 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    19441 acttactttc cttaactctt cgccttaacg caaaatctca cactgatgat cctgaatttc
    19501 ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt gtcgccagca atacaaatga
    19561 gttatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    19621 tacttcttct gccagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    19681 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    19741 gttcgccaga ttgttatcca gttgtttgcg cacatcagga cgaatacaag agatatgaat
    19801 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    19861 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    19921 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    19981 tggcatcaac agatattgca gtgggccatt aagatctttt aaaaccacgt atccggcatt
    20041 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    20101 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    20161 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    20221 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    20281 acccggtcgc gagaaaccac cgggtttgat aattatcctg gagaaatcag cgtttgctga
    20341 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    20401 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    20461 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    20521 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    20581 cgaccaccga cacggttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    20641 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaagcg atcagtacat
    20701 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    20761 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    20821 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    20881 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    20941 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    21001 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    21061 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    21121 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    21181 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    21241 taagaagctg aatatccttt gccataacgt tggttgccgc cagcaaaaat gttaacccta
    21301 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    21361 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    21421 gggggttaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttatcgat
    21481 gccggatgcg gcgcgaacgc cttatccggc ctacaaaaat ttgtaaattc aataaattgc
    21541 agaattcacg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    21601 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    21661 gccagtcgcc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    21721 ccagctgttc gttctggata cctacgcaac gaccgccgta acctgccagc agcagatcga
    21781 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    21841 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    21901 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    21961 ctttcgcgat acccgctttg atttcgttta ccagatcttc acggctgaat tcaacttccg
    22021 gaaccacaac gaattcacag ccaccggcaa tggccgcagc caacgtcaga tcgccacaat
    22081 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    22141 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    22201 ctttgatgtc gttgtcgata gtgccaggca gaccgatgca cgggaagccc atttcggtca
    22261 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    22321 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    22381 gcgcagaacc gaggaacgta ccaccacggt tgatcatgtc agaaacgctg taacggtcta
    22441 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaatgccc attacttcca
    22501 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    22561 caccgccgct tgtcaacaca ccgattttct taatcatgac tacctctgaa ctttggaatg
    22621 caaaataaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    22681 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    22741 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    22801 cctccctggg tacgacggaa caggggtcct gatggataat tacatccgat cccggaaaac
    22861 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    22921 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    22981 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    23041 cctcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    23101 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    23161 cgtaccagga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    23221 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caacgctgaa
    23281 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    23341 gatctgtcat cggtgttgga gatatcagat gttgaatacc cgtcaaaaac aggaatagtg
    23401 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactctgct ttaccgtgac
    23461 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    23521 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    23581 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    23641 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    23701 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    23761 taaaaaaaac ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    23821 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    23881 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    23941 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    24001 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    24061 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    24121 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    24181 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    24241 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    24301 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    24361 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    24421 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    24481 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    24541 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    24601 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    24661 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    24721 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    24781 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    24841 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    24901 gaatccaggc cgtcaggaag ccagcttcga cggacaaacg ctggagttaa ccggcactga
    24961 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    25021 aagccaggaa gtgctgggca aacgcctgac gcctttcgac cgcgctatcg atatgcacat
    25081 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    25141 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    25201 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    25261 ctcgattcac gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt
    25321 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    25381 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    25441 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    25501 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    25561 ggtccgttct ccgtacgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    25621 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgttatt actgctgatt
    25681 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    25741 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    25801 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    25861 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    25921 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    25981 gaaagcaagg aactggagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    26041 gatctgttgg tgatgtcccg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    26101 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    26161 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    26221 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    26281 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    26341 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    26401 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    26461 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    26521 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    26581 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    26641 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    26701 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    26761 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    26821 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    26881 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    26941 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    27001 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    27061 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    27121 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    27181 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    27241 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    27301 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    27361 accatattta ttttatgtca ggcgtgctgt tttcgcggta agtatccagg caggaactca
    27421 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    27481 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    27541 aggtaataat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    27601 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    27661 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    27721 atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga
    27781 tccccagcaa accagtctgg gcaattacag tcaaatcaat ggtattgccc agtgcgaagg
    27841 caaagaatgg aatcagcgtc tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    27901 ggttccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    27961 caaacgaggc aatccctgca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    28021 acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc atgatggaag
    28081 cgtaaagtcc gccattggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    28141 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    28201 cccacgcgac ggcaattttt gttaccacca gcgtaccgga tttacgcaat accgttcccg
    28261 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    28321 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    28381 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    28441 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtca
    28501 tttctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    28561 agtgatcaaa accacatcaa ttgaaacact gttttatttt tatcggatca ttgtttttat
    28621 tttgaaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    28681 tgcggcgtaa acgcctcatt gcagctggcg gcaaatgatt attttttcgc cgcgaaacgc
    28741 gccgcagctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cgggcggcgg
    28801 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    28861 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    28921 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    28981 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    29041 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    29101 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    29161 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    29221 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    29281 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    29341 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggttcatta
    29401 tagttaatta aatgatattg aaaatgatta tcaatgccgt acttttcgta agggtatggt
    29461 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    29521 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    29581 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    29641 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    29701 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    29761 gattattctg ccgtgggcca tcagcgccct gttactaccg aatttctggg cgtattacag
    29821 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    29881 taatatcaac tatggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc
    29941 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    30001 tgtgctgatt aacaccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat
    30061 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    30121 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    30181 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcactagg
    30241 cgtcgatcca ctgtatgtcg ctctgccaag ctatgtcatc atcatgggcg gcggcgcgat
    30301 catcaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    30361 cgacttctcg ctggcaaaac cgctgatcac tcacaatgtg ttactctcag cactgggcgg
    30421 gttgatgtgg tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca
    30481 atatgactac atcagttgga tgctgcatat gagcttctat gtattgtgcg gcggtatcgt
    30541 cgggctggtg ctgaaagagt ggaacaatgc aggacgccgt ccggtaacgg tgttgagcct
    30601 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    30661 tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac
    30721 caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag
    30781 gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca gatattgatt
    30841 gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc
    30901 ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca gccgggtaat
    30961 cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg
    31021 gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt tagcaaacga
    31081 cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct gcgccatccc
    31141 cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc
    31201 ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag
    31261 atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga gatcgccacg
    31321 ggtaatgcga taagggcgat cgttgagtac atgcagacca ttaccgcgcc agacaatcac
    31381 cagctcacaa aaatcatgtg tatgttcggc aaaaacatct tgcggataac ggtcagccac
    31441 agcgactgtc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc
    31501 caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac
    31561 gttgagaaga ttcgcgttat tgcagaaaac catcccgtcc ctgacgaata tcacgcggtg
    31621 accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccacagc
    31681 gataggcgat atcagtaacg ctggcctcgc tgtggcgtag tagatgtcgg gctttcatca
    31741 gtcgcaggcg gttcaggtaa cgctgaggcg tcagtcccgt ttgctgctta agctgccgat
    31801 gtagcgtacg cagcgaaaga gaaaattgct ccgccacggc atcccaattc acctcatcgg
    31861 caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt
    31921 tctcctgcaa actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac
    31981 tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc
    32041 gcacctgctg caatacgctg tggttaacgc gccagtggga aggatactgc ccatccagct
    32101 cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca
    32161 gcacattagt cagacacaga ttatcggtat gttcatacag atgccgatca tggtcgcgca
    32221 caaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg
    32281 tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct
    32341 gcgggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat
    32401 gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt
    32461 caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat
    32521 acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaattca gcaaattgtg
    32581 aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag
    32641 aaggtcgcga attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt
    32701 atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg
    32761 gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat
    32821 gggctgcata gtcagaacgg ctatgtcacc tgggatgtgg atagcctgga aagtgccatt
    32881 cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat tgggattgat
    32941 acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg cctgcccgtt
    33001 gcttatcgcg atagccgcac caatggcctg atggcgcagg cacaacaaca actcggcaaa
    33061 cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgct ttatcagtta
    33121 cgggcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc tctgctaatg
    33181 ccggattatt ttagttatcg ccttaccggc aagatgaact gggagtacac caacgccaca
    33241 accacgcaac tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc
    33301 ggtgccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat aggtcactgg
    33361 atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga taccgccagc
    33421 gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc tggtacctgg
    33481 tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct ggcagccaac
    33541 atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta
    33601 tggctgcttc agcgggtgct taaggagcgg aaaatcaacg atctcccggc gcttatcgtc
    33661 gcgacacagt cacttccggc ctgccgcttc atcatcaatc ccaatgacga tcgctttatt
    33721 aatcctgagg cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc
    33781 ccggaaagtg atgctgaact ggcgcgctgc attttcgaca gtctggcgct gctgtatgcc
    33841 gatgtgttgc atgagctggc gcagctacgc ggtgaagatt tctcgcaact gcatattgtc
    33901 ggcggaggct gtcagaacac gctgctcaac cagctatgtg ccgatgcctg cggtattcgg
    33961 gtgatcgccg ggcctgttga agcctcgacg ctcggcaata tcggcatcca gttaatgacg
    34021 ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg tcagcaccac cgcgaatctg
    34081 accaccttta cccctaatcc tgacagtgaa attgcgcact acgtggcgcg gattcactct
    34141 acacgaaaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc tgggagctgg
    34201 cgaaacagcg tttcgcggcg gtggggattg atgtcgagga ggcgctgcgc caacttgatc
    34261 gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt gaaaacccgg
    34321 aaggttcgct gaccgggggg attcaggcca caggcaatta tccgggcaaa gcgcgtaatg
    34381 ccagtgagct acgtgccgat ctggaacagg ctatgcggct gattccgggg ccgaaacggc
    34441 ttaatttaca tgccatctat ctggaatcag atacgccagt ctcgcgcgac cagatcaaac
    34501 cagagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt ctggatttta
    34561 acccctcctg cttttcgcat ccgctaagcg ccgatggctt tacgctctcg catcccgacg
    34621 acagcattcg ccagttctgg attgatcact gcaaagccag ccgtcgcgtt tcggcctatt
    34681 ttggcgagca actcggcaca ccatcggtga tgaacatctg gatcccggat ggtatgaaag
    34741 atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct ggcagctctg gatgaggtga
    34801 tcagcgagaa gctggatccg gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca
    34861 ttggcgcaga gagctacacg gttggctcca atgagtttta catggggtat gccaccagcc
    34921 gccagactgc gctatgcctg gacgccgggc acttccaccc gactgaagtg atttccgaca
    34981 agatttccgc cgccatgctg tatgtgccgc agttgctgct gcacgtcagc cgtccggttc
    35041 gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcaatt gccagtgaga
    35101 ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct tgacttcttc gatgcctcta
    35161 tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc ctgctgcgtg
    35221 cgttgctgga acctaccgct gagctgcgca agctggaagc ggcgggcgat tacactgcgc
    35281 gtctggcact gctggaagag cagaaatcgt tgccgtggca ggcggtctgg gaaatgtatt
    35341 gccaacgtca cgatacgcca gcaggtagcg aatggctgga gagcgtgcgg gcttatgaga
    35401 aagcgatttt gagccagcgt gggtaaacac tgccggatgc ggcgcgagcg ccttatccgg
    35461 cctacgggtc gtgcatctga caacaccgaa tttacaggaa cacagaacat gcaaaacatt
    35521 acccagtcct ggtttgtcca gggaatgatc aaagccacca ccgatgcctg gctgaaaggc
    35581 tgggacgagc gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgctt
    35641 tatcacgaca acttccaccc acaaccgcgc tatattccgc tcagccagcc catgccttta
    35701 ctggcaaata cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt
    35761 gatcctgcag ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt
    35821 ctctgggggt taaccaacga agccgttccc acttccgaac ttccggctca cttcctttcc
    35881 cactgcgagc gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc
    35941 accaacctga tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa
    36001 ctgtgggaag gcagcaccga gtgtctggta gtgttcccgg atggcgttgg cattttgccg
    36061 tggatggtgc ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat
    36121 tcgctggtgt tgtggtcctt ccacggcgtc ttcggcagcg ggccgacgct ggatgaaaca
    36181 ttcggtttaa tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg
    36241 ggcggcatga aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc
    36301 gttacaccac tagccagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt
    36361 gtcatgcagg ttaaccccga cgcccacgaa gagtatcagc gtcggcataa ccccatctgg
    36421 ccggaactgg aagcagtgtt gaaatctcac ggtgcgcata actacgccat ttacctcgac
    36481 aaagcgcgta atctgctgtt tgccacggta gagattgaat ctgaagaacg gtggaatgcg
    36541 gtagccagta ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct
    36601 aacccggata acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg
    36661 cccaatgtgc cggagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac
    36721 atattacatc tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata
    36781 atgaataacc cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc
    36841 gcaaatctga cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca
    36901 tcacttaatc gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg
    36961 gcagctctta ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt
    37021 ctgaagcagt tggcgacaat agcgttacaa aacggtttta tcaccgactc acaccagttc
    37081 ctgcaaaccc tgctgctgcg cgaaaaaatg cactctactg gatttggttc cggtgtcgcc
    37141 gtgccgcacg gtaaaagcgc ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg
    37201 caggctattg actggaaagc cagcgatggc gaagacgtca attgctggat ctgcctcggc
    37261 gtgccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt
    37321 atccaccagg attttattca tcaactgcaa cagggcgacg ccgaccaggt gcttgccttg
    37381 ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag tcatccttac gtattgtcgc
    37441 gatcaccaac tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca
    37501 gaaagcccgt tctctcggcc ataccataaa agtggaaact caagggtcca gtggcgttga
    37561 aaaccgctta tccagcgaag agattgccgc tgccgattac gtcattctcg ctaccgggcg
    37621 tggcctgagc ggtgatgatc gcgcgcggtt tgccgggaag aaagtttatg agattgccat
    37681 ctcccaggcg ttgaaaaata tcgaccagat tttcagcgaa ttaccgacaa actcgcagct
    37741 ttttgccgca gatagcggcg tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat
    37801 gagccacctg atggcgggcg tctctgcggc gctgccgttt gtcatcggcg gtggtatcct
    37861 ggtggcgctt gccaacatgc tggtgcagtt cggtttgccg tataccgata tgtcgaaagg
    37921 cgcgccgtcg tttaccttgg tagttgaatc catcggttac cttggcttca cctttatgat
    37981 ccccatcatg ggcgcttata ttgcctcgtc gattgccgat aaaccggctt tcgctccggc
    38041 gtttctggtt tgctatctgg cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg
    38101 attcctcggc gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgcaa
    38161 agtgcgcctt ggcaaagcat tacaaccgct gctcggttca atgctgatcc cgttcgttac
    38221 tctgctggtt ttcggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat
    38281 gggcgggctg ctccacttcc tgaataccat tcctccatca atgaagtttg cggcagcgtt
    38341 tctggtcggt gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt
    38401 cttctgcttc tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt
    38461 cgcgttgatg ccgcccgttg cggcgggtct tgcgacgttc attgcgccca aactgtttac
    38521 ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga
    38581 accggctata ccttacgctc tggcagctcc gttgccgatg attaccgcca atacgctggc
    38641 gggtggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cacctggttt
    38701 gggtatcttt gacccgctga tcggcctgat gtcgccggtg ggttcgtttt atctggtgct
    38761 ggcgattggc ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg
    38821 ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt
    38881 tgtgcgaagc cagcgccgtc agcggcgatg aacaggaagt tcgcgacatt ctgataaaca
    38941 cgctggaacc ttgcgttaat gagatcacct ttgatggtct gggcagcttt gttgcccgta
    39001 aggggaataa aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg
    39061 tcacccacat cgacgagagc ggttttctgc gtttcaccac cattggtggc tggtggaatc
    39121 agtcgatgct caaccaccgg gtaaccatac gcacacacaa gggagtgaaa atccctggtg
    39181 tgattggttc cgtcgcgcct catgcgttaa cggaaaagca aaggcaacag ccgctgtcat
    39241 ttgatgagat gttcattgat attggcgcga acagtcgcga tgaggtggaa aaacgcggtg
    39301 tggcgatggg cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag
    39361 tggtcggcaa ggcgctggat aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga
    39421 cggtgaataa tcccgaaatt acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc
    39481 tacgcggggc acaaacctcg gcggaacaca ttaaaccgga tgtggtgatt gtgctggata
    39541 ccgctgtcgc aggtgatgtt ccgggcattg ataacattaa atacccgctg aaactgggcc
    39601 aggggccggg gctgatgctg tttgacaagc gctacttccc caaccagaaa ctggtagcag
    39661 cgtttaaaaa ctgtgccacg cagaatgatt taccgctgca attttccacc atgaaaaccg
    39721 gagcgacgga tggcgggcgc tacaacgtga tgggcggcgg gcgtccggtt gtcgcgctgt
    39781 gtctgccaac tcgttatctg cacgccaaca gcggtatgat ttcaaaagcc gattacgatg
    39841 ctctgctcac gctgatacgg ggttttctga cgaccttaac tgcggagaaa gtcaacgcgt
    39901 ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct
    39961 gctggagcaa cagccgcgca cgcctggcga gctggcgcaa cagactggcg tttcaggcag
    40021 gaccatcctg cgtgatattg actatctcaa cttcaccctt aacggcaaag cccgcatttt
    40081 cgccagtggc agtgcgggct atcagctgga aatcttcgag cgccgcagct tttttcagtt
    40141 gctgcaaaag cacgataacg acgatcggct gctggcgctg ttattactga atactttcac
    40201 tccccgtgcg caactcgcct cggcgcttaa tttgccagaa acgtgggtag cagagcgtct
    40261 gccccggtta aaacagcgtt atgaacgcac ttgttgcctg gccagccgcc ctggtttggg
    40321 ccatttcatt gatgagacag aagagaaacg cgttatcttg ctggcgaact tgctgcgcaa
    40381 agatccgttt ttaattccgc tggcgggcat aacacgagac aaccttcagc atttatccac
    40441 ggcctgcgac aaccaacacc gctggccgct catgcagggt gattatctct ccagcctgat
    40501 tctggcgatt tacgccctgc gtaatcaact gaccgatgag tggccgcaat atcccggtga
    40561 cgagataaaa caaatcgttg aacatagcgg tctgtttctc ggtgataatt ctgtaagaac
    40621 cctgacgggt ttgatagaga aacagcatca gcaagcgcag gtaatttcag ccgatcatgt
    40681 gctggggttg ctgcaaaggg tgccaggcat cgcgtcattg aatattattg atacgcagct
    40741 ggttgagaat attaccgggc atttattacg ttgccttgcc gcaccggtgt ggattgctga
    40801 gcaccgccaa agcagcatga ataacctgaa agcagcctgg ccagcggcct ttgatatgag
    40861 tctgcacttt attacgctac tgcgtgaaca gctcgatatt ccccttttcg acagcgatct
    40921 gatcggtttg tattttgcct gtgcgctgga acggcatcaa aacgaacgcc agccgatcat
    40981 tttgctctcg gaccagaacg cgattgccac tattaatcag ctcgccattg agcgcgatgt
    41041 tttacattgt cgggtgatta ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga
    41101 gattgagccg ttattgatca ttaacaacag ccattattta ctggatgacg cggtaaataa
    41161 ttgcatcacc gtaaaaaata tcattacggc tgccggtatc gaacaaataa aacattttct
    41221 ggcgacggca tttattcgcc agcaaccgga gcgttttttc tctgcccccg gaagttttca
    41281 ttattcgaat gtacgcggtg aaagctggca acatattacc cggcaaattt gtgcgcaatt
    41341 agtggcacaa caccatatta ccgccgatga agcgcaacgg atcatcgccc gcgaaggcga
    41401 aggtgaaaac ctgattgtta atcgcctcgc catcccacat tgctggagcg aacaggagcg
    41461 acgttttcgt ggatttttta ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat
    41521 taaccatgtc ttgatcgcct gcgccgccgc cgatgcgcgt cacgagttga aaatattcag
    41581 ctatctggca agcgtattgt gtcagcatcc ggcagaggtt attgccgggt taacaggata
    41641 tgaggcattt atggagttac ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac
    41701 actgcattta tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg
    41761 tactcgcgta atgctctcgg gaaagagtct tcgaccatcc agcttgagtt tgcgtcctga
    41821 agttgctctt tatcccagtc agagtacggg actttatctc tgatgcggcg aacgtatttt
    41881 ttagcctcga gttgatactg gcctgccgaa ttaataaacg agaaattcat gccgccttta
    41941 tccgcttctt tggcctgcgc caccagcgtt tccagttcag acactttctt cattgccgct
    42001 tctgcatcaa acttatcttg cgatatcagg ttattaattt gtttcgctga aatcatgact
    42061 gccagagagt agtagtggaa tgttttccct tctctttctt caatattttt cagttccgca
    42121 agctgacgct tatcattaat ttcctgaatc gccgcatgat aagattcggc tacaggttca
    42181 aaggcttcca gattttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg
    42241 tctttataat tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgcc
    42301 acggcagcat cgatataact caccgccacg ccatcaattg gttgcagcgc aggcgttaac
    42361 gccacagcac tttttacgcc tttttcacac tcagcgaggt tggattcact aatgccgtag
    42421 atgccataaa cagtacgctc ttcaccggta ggcccctgtt taaaatcttt cagccagtca
    42481 gcataacgcg ccagactgcg ctgtaccggg atctgtaact tgttgtagca cttgatatag
    42541 acatccattt tttcgtcgaa aaccgcttgt tcatcgacca ccggttgcgc aggtgtttca
    42601 ggtttagctt ctgcttttgc aacgggagct tcagtaggtt tcgcttcagg agctggtgcg
    42661 gcaggttgac tattggcagg cgggagcgtt tctgtttcgg cttttttatc atcacaaccc
    42721 gtcagaccga gggacatgat ggcgactata atagcggaag ataataaatt ccttttcatt
//
LOCUS       DAPHJE010000028        41706 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPHJE010000028 DAPHJE010000000
VERSION     DAPHJE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.028"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..1886)
                     /gene="gss"
                     /locus_tag="RH222_001515"
     CDS             complement(27..1886)
                     /gene="gss"
                     /locus_tag="RH222_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV3796563.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2091..2957
                     /gene="yghU"
                     /locus_tag="RH222_001516"
     CDS             2091..2957
                     /gene="yghU"
                     /locus_tag="RH222_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV3796564.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3080..3328)
                     /gene="hybG"
                     /locus_tag="RH222_001517"
     CDS             complement(3080..3328)
                     /gene="hybG"
                     /locus_tag="RH222_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV3796565.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3341..3682)
                     /gene="hypA"
                     /locus_tag="RH222_001518"
     CDS             complement(3341..3682)
                     /gene="hypA"
                     /locus_tag="RH222_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV3796566.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3675..4163)
                     /gene="hybE"
                     /locus_tag="RH222_001519"
     CDS             complement(3675..4163)
                     /gene="hybE"
                     /locus_tag="RH222_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV3796567.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4156..4650)
                     /locus_tag="RH222_001520"
     CDS             complement(4156..4650)
                     /locus_tag="RH222_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV3796568.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4650..6353)
                     /gene="hybC"
                     /locus_tag="RH222_001521"
     CDS             complement(4650..6353)
                     /gene="hybC"
                     /locus_tag="RH222_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV3796569.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6350..7528)
                     /gene="hybB"
                     /locus_tag="RH222_001522"
     CDS             complement(6350..7528)
                     /gene="hybB"
                     /locus_tag="RH222_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV3796570.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7518..8504)
                     /gene="hybA"
                     /locus_tag="RH222_001523"
     CDS             complement(7518..8504)
                     /gene="hybA"
                     /locus_tag="RH222_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV3796571.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8507..9625)
                     /gene="hybO"
                     /locus_tag="RH222_001524"
     CDS             complement(8507..9625)
                     /gene="hybO"
                     /locus_tag="RH222_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV3796572.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9814..10101)
                     /locus_tag="RH222_001525"
     CDS             complement(9814..10101)
                     /locus_tag="RH222_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV3796573.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10220..11107)
                     /locus_tag="RH222_001526"
     CDS             complement(10220..11107)
                     /locus_tag="RH222_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV3796574.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11264..12304
                     /gene="gpr"
                     /locus_tag="RH222_001527"
     CDS             11264..12304
                     /gene="gpr"
                     /locus_tag="RH222_001527"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV3796575.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12344..12838)
                     /locus_tag="RH222_001528"
     CDS             complement(12344..12838)
                     /locus_tag="RH222_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV3796576.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13029..13913
                     /gene="yghA"
                     /locus_tag="RH222_001529"
     CDS             13029..13913
                     /gene="yghA"
                     /locus_tag="RH222_001529"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV3796577.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14185..14610)
                     /gene="exbD"
                     /locus_tag="RH222_001530"
     CDS             complement(14185..14610)
                     /gene="exbD"
                     /locus_tag="RH222_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV3796578.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14617..15351)
                     /gene="exbB"
                     /locus_tag="RH222_001531"
     CDS             complement(14617..15351)
                     /gene="exbB"
                     /locus_tag="RH222_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV3796579.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15603..16790
                     /gene="metC"
                     /locus_tag="RH222_001532"
     CDS             15603..16790
                     /gene="metC"
                     /locus_tag="RH222_001532"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV3796580.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16930..17589
                     /locus_tag="RH222_001533"
     CDS             16930..17589
                     /locus_tag="RH222_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3796581.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17629..18528)
                     /gene="yqhC"
                     /locus_tag="RH222_001534"
     CDS             complement(17629..18528)
                     /gene="yqhC"
                     /locus_tag="RH222_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV3796582.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18722..19885
                     /gene="yqhD"
                     /locus_tag="RH222_001535"
     CDS             18722..19885
                     /gene="yqhD"
                     /locus_tag="RH222_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV3796583.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19990..20817
                     /gene="dkgA"
                     /locus_tag="RH222_001536"
     CDS             19990..20817
                     /gene="dkgA"
                     /locus_tag="RH222_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV3796584.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21017..21943
                     /locus_tag="RH222_001537"
     CDS             21017..21943
                     /locus_tag="RH222_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV3796585.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21994..22251
                     /gene="yqhH"
                     /locus_tag="RH222_001538"
     CDS             21994..22251
                     /gene="yqhH"
                     /locus_tag="RH222_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV3796586.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22294..24513)
                     /locus_tag="RH222_001539"
     CDS             complement(22294..24513)
                     /locus_tag="RH222_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV3796587.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24624..26036)
                     /gene="ftsP"
                     /locus_tag="RH222_001540"
     CDS             complement(24624..26036)
                     /gene="ftsP"
                     /locus_tag="RH222_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV3796588.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26111..26848)
                     /gene="plsC"
                     /locus_tag="RH222_001541"
                     /gene_synonym="parF"
     CDS             complement(26111..26848)
                     /gene="plsC"
                     /locus_tag="RH222_001541"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV3796589.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27082..29340)
                     /gene="parC"
                     /locus_tag="RH222_001542"
     CDS             complement(27082..29340)
                     /gene="parC"
                     /locus_tag="RH222_001542"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV3796590.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29478..31085)
                     /locus_tag="RH222_001543"
     CDS             complement(29478..31085)
                     /locus_tag="RH222_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3796591.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLEQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQTPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31218..31613)
                     /gene="mqsA"
                     /locus_tag="RH222_001544"
     CDS             complement(31218..31613)
                     /gene="mqsA"
                     /locus_tag="RH222_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HDV3796592.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            complement(31615..31911)
                     /gene="mqsR"
                     /locus_tag="RH222_001545"
     CDS             complement(31615..31911)
                     /gene="mqsR"
                     /locus_tag="RH222_001545"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HDV3796593.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            complement(32116..32598)
                     /locus_tag="RH222_001546"
     CDS             complement(32116..32598)
                     /locus_tag="RH222_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3796594.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32651..33043)
                     /gene="ygiW"
                     /locus_tag="RH222_001547"
     CDS             complement(32651..33043)
                     /gene="ygiW"
                     /locus_tag="RH222_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV3796595.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33195..33854
                     /gene="qseB"
                     /locus_tag="RH222_001548"
     CDS             33195..33854
                     /gene="qseB"
                     /locus_tag="RH222_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV3796596.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPDKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33851..35200
                     /gene="qseC"
                     /locus_tag="RH222_001549"
     CDS             33851..35200
                     /gene="qseC"
                     /locus_tag="RH222_001549"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV3796597.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35310..35891
                     /gene="mdaB"
                     /locus_tag="RH222_001550"
     CDS             35310..35891
                     /gene="mdaB"
                     /locus_tag="RH222_001550"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV3796598.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35922..36236
                     /locus_tag="RH222_001551"
     CDS             35922..36236
                     /locus_tag="RH222_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV3796599.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36280..37611)
                     /locus_tag="RH222_001552"
     CDS             complement(36280..37611)
                     /locus_tag="RH222_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796600.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTMTDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPDTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVKLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAYLDYQPGV
                     EQTPQALWGICLLMTIFPAILNLITGLIMRFYLINNEFYEQIKARLQTAEE"
     gene            37898..38650
                     /locus_tag="RH222_001553"
     CDS             37898..38650
                     /locus_tag="RH222_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3796601.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     QEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            38664..39740
                     /locus_tag="RH222_001554"
     CDS             38664..39740
                     /locus_tag="RH222_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV3796602.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLVEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALQAQEK"
     gene            39740..40738
                     /locus_tag="RH222_001555"
     CDS             39740..40738
                     /locus_tag="RH222_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDV3796603.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNFPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            40743..41657
                     /locus_tag="RH222_001556"
     CDS             40743..41657
                     /locus_tag="RH222_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV3796604.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
BASE COUNT        10555 a        10820 c        10337 g         9994 t
ORIGIN      
        1 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
       61 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      121 ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      181 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      241 tgatggctga cgaggtcgat attgctgcca cagcgaccgg cgatcggttt cactgcgtaa
      301 cctgttttca ccagttcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      361 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      421 ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      481 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      541 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagccg tccttcccca
      601 tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa gatacgcgtt
      661 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      721 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      781 agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      841 caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc gttgtattcg
      901 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
      961 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1021 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1081 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgataa
     1141 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag cggatctttt
     1201 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1261 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1321 atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca gccgttttcg
     1381 accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1441 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1501 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1561 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1621 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1681 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1741 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1801 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1861 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1921 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     1981 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2041 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2101 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2161 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2221 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2281 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2341 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2401 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2461 atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2521 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt tttggtcact
     2581 tttaccatta cgcaccggta aagattgagt acgccatcaa ccgctttacc atggaagcca
     2641 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttgtt gcgggcgatg
     2701 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caacgtggtg ttaggtggtg
     2761 tgtatgatgc cgctgagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2821 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2881 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     2941 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3001 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca
     3061 ggcctacgaa agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt
     3121 ctaatgtggc tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca
     3181 gcacccactg gcccagtaga tcggcagggt taccttcaca aatcagggcg atattcacgt
     3241 cgcgcttgat accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca
     3301 gcacctggcc tggaacgcca atacacatta ttaactccgg ttattcaact tcaatacttt
     3361 tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact
     3421 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccag gcctgggcgg
     3481 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
     3541 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg
     3601 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
     3661 ggcaaagaga caactcatgc attttcgcca ctcctgcgac caaacagcag ggcgcgacgc
     3721 cctgcgtgtg gtacatccgg attcgtgact ggcagcgaaa ggatcattcg tgcgcagtca
     3781 tccgtcaggc gttggccctc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa
     3841 caggagagat attgcgaaac accgtccagt tcgccaacgg taaaggtcat agtgccatac
     3901 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
     3961 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
     4021 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
     4081 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
     4141 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgccagattc
     4201 acgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
     4261 taagccgatg tgtggctcca gcgattccgg gatcacgccg accagggtga gctttttcgg
     4321 aaactcaccg gtgaagcgca gggccgacaa gacgtcggcc aggccaagct gatgcggaga
     4381 gattttgttg gtaaacaacg ccggaacttc ttcatcgcgc aggatcatca tcgttcccgg
     4441 cgcgctcttc ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
     4501 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
     4561 gtatcgttgc tctaacgctt cgacaatccg cacaccgatg gcttcatcgg tcagcaaaat
     4621 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
     4681 ccgtcagcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
     4741 cgcaccactt ccagcggttt attcggatcg gcaaccggtg tacccaccag cgactgctcg
     4801 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaacc
     4861 gcctggtagt tactgatgat accgtctttg atcaccatcc agtgagagag cataccgcgc
     4921 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggcttcaca
     4981 aaggtggtat gatcgccttt accgatattg gtgatcagtg cactgtattg attttgcagg
     5041 atatcctgca attcgcagca gtgaacggta cgaccaataa tacggcccag cgtagagtgc
     5101 agttgcgcca cttcaagcgt gttaccagtc agtttctgat aaatcgcaac gatttcattc
     5161 agtttgttct gggtagactc gcgtcctgcc gccagtttca ccagcatgtt agccagcgga
     5221 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
     5281 tcagaccaac catcataagc cggaatggtg gtaccttccc acggtgcctg cggcgcttcg
     5341 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
     5401 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc gcccgggaac
     5461 aggaagctgc cgtttttact gtcggtcggg aattccggca cgctcaggta gttcaccgca
     5521 cctttaccgc gcgtcagcca ttccgggtag aacgcggcaa taactgcggt atcaacctta
     5581 taaacctgtt caacaaagtc gctcagcttg tcgatgaaag acttgatgta catcaggcgc
     5641 tcaaggttca gcacgcccaa accgtcgagg ttgatcgggt tcgcgacacc acctaccgcc
     5701 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     5761 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     5821 ttcatcgccg ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc
     5881 agatctttga tcttgttctg aactttggtg aactcttccg gactgttcag gtgccaggtc
     5941 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
     6001 tccacccagt ccagcgccga aagctgatag aaatgaacaa tatggtcatg cgtggtgtgc
     6061 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
     6121 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
     6181 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     6241 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     6301 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     6361 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     6421 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     6481 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccagc gaataggtca
     6541 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atgcgggaat
     6601 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
     6661 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     6721 taagctcgcc aaagcgcagc acgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     6781 gcttaacaaa cagactcttt tcatccggac cgttgccacg cagacccgcc tgcaccagcg
     6841 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     6901 gcaacatttc atagctctgc cacaacggat gcaccttgta gcccgccgag atcatcagcg
     6961 accccattga agactggtgc atggtcggca gcagcgcacc gagcgcgatg atgaagaaca
     7021 tcaccttgtt tagtcgctgt agcgacacct tccaccccag acgttcaaac agtgccggag
     7081 caaactccag tgccatcacg ccgatataga tggtcataca gaccgccgtc tcgaacagta
     7141 ccgagttcac gttgaagtgg cccggaatgt agaagtacgg caggttccag tagcgaccca
     7201 cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg
     7261 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     7321 agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg
     7381 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     7441 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     7501 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     7561 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     7621 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     7681 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
     7741 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     7801 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
     7861 ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact
     7921 tctacgcagc caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc
     7981 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga
     8041 caggcgacca tgcagtaacg gcagccggtg cacacgtctt tgtcgtaatg gacaatgccg
     8101 gttttcggat cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg
     8161 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     8221 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
     8281 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
     8341 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     8401 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     8461 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     8521 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     8581 cggcaacgag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
     8641 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     8701 gctgatggat gcctttatgg aagccgatac cttcttcgtt acagccatag catgggtgac
     8761 caatcgctac cggccacacg ccgccaacat cgcagaattg cagcgttgag cagttgccgt
     8821 aagtttctgg ccctttacag ccgaggtggt acaggcacca gccttcacgg tggccttcat
     8881 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     8941 gaatcagacg gccataggcg aaggtcggac gatttttgtc atccagtttc ggcggtttgc
     9001 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     9061 gaatattgat aacggttttg cctggcagaa cttcttgcag gctgactgcg ccagttgggt
     9121 taactccagc tgcggcaaca ccgccccacg cagagcagga accgatagcg ataatcgctg
     9181 ctgcgccttc tgccgctttg cggatgtgat ccacaatcgg ctcaccagca accatgcaat
     9241 aaataccgtt atcttttaat gggatggaac catccacaac caacacatac tgccctttgt
     9301 acttctcaag tgcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
     9361 cttcgtgata ctccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     9421 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     9481 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     9541 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     9601 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgaagacc tggcatatat
     9661 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     9721 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     9781 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     9841 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
     9901 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
     9961 ggtcttttcg tacatccggt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    10021 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    10081 tttaccaaaa tggttattca tcgttcacct cattcgctat atatcgccat atatagcgat
    10141 attttaggca aagaacgact tttgcgcacg ctattttttc atgcttgttt aagacgtaat
    10201 ccagtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
    10261 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    10321 taacccccgg atagatatac gcctcataaa ccttattatt ggctttcaac gccgcctcgt
    10381 aagcaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
    10441 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    10501 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
    10561 taatacccac tttgcctgcc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga
    10621 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    10681 ttcccggata acctccaacg gaacttaagc cgtcaggtgc cagggcgata taccccgcct
    10741 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
    10801 ctaccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
    10861 cgccgtgacc atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa
    10921 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
    10981 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gagattttcc
    11041 cgtgagcgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
    11101 gcggcatcgt tgcactcctc aactggtgtt tttttaagca aagcataagc acgtgttttt
    11161 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    11221 ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa
    11281 tcccgaacgt tacgggcaga tgcaataccg ctattgcgga aaaagcggtt tacgcctgcc
    11341 cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    11401 tgcgatcctg cgtaaagcgt ttgatttggg cattacgcac tttgatttag ccaacaatta
    11461 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcggg aggattttgc
    11521 cgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
    11581 ttacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    11641 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    11701 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    11761 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
    11821 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    11881 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
    11941 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgtatcg
    12001 tgaagggaat aaagttcgtg gtctgacacc gaaaatgctt accgaagcca acctcaacag
    12061 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    12121 aagctggttg ttgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
    12181 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    12241 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
    12301 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
    12361 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
    12421 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaggt
    12481 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    12541 ttttccccag ccagttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
    12601 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    12661 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    12721 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    12781 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    12841 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
    12901 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
    12961 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    13021 gaaaaacgat gtctcatcta aaagacccga ccacgcagta ttacactggt gaatatccca
    13081 aacagaaaca accgacgcca ggcatccagg cgaagatgac accggtaccg gattgcggcg
    13141 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    13201 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
    13261 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
    13321 gcggtcgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    13381 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    13441 aacaggttgc cattcctgat attgcagacc tcaccagcga acagtttcaa aagacctttg
    13501 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    13561 gtgcaagtat catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
    13621 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
    13681 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
    13741 tttctggcgg acaaacgcag gataagatcc cgcagtttgg tcagcaaacg ccgatgaaac
    13801 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
    13861 gctacgtcac cgcagaagtg cacggtgtgt gcggcggcga gcatttaggt taaaaaaatg
    13921 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
    13981 acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc
    14041 tgataagcgt agcgcatcag gcattgagca cctaatgccg gatgcggcgt gaacgcctta
    14101 tccggcctac aaacgcacga agattcaaca tgttgcaata gattttgtag gcctgatgag
    14161 cgtagcgcat caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc
    14221 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    14281 gacggtttta tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt
    14341 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    14401 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    14461 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    14521 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    14581 gtcgaggttt tcgttaagat gcattgccat cggatattat cctgcgcgta atttttgtgc
    14641 gacacgaacc ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa
    14701 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    14761 tacgttatag ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag
    14821 agcttctgcg atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc
    14881 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc
    14941 gccgatggtt gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg
    15001 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    15061 cagttccagc tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa
    15121 atcagcggcg atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc
    15181 gcgcttaagg cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca
    15241 ggtgactacg gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg
    15301 ctgatacata ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    15361 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt
    15421 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    15481 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    15541 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaat aggaatcccg
    15601 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    15661 ctcttggcgc ggtaaatagc gtgattcagc gcgcttcttc gctggtattt gagagtatgg
    15721 aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg
    15781 gaacgttaac ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    15841 gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc gattcttgct tttgtcgaac
    15901 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    15961 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    16021 tcgttaagca tctgcagcca aacactaaaa tcgtgtttct ggaatcgcca ggctccatca
    16081 ccatggaagt ccacgacgtt ccggcgattg ttgccgccgt acgcagtgtg gtgccggatg
    16141 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    16201 gcatcgatgt ttctattcaa gccgccacca aatatctggt tgggcattca gatgcgatga
    16261 ttggcactgc cgtgtgcaat gcccgttgct gggagcagct acgggaaaac gcctatctga
    16321 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    16381 gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac
    16441 atccgcaagt agcgcgagtt aaccaccctg ctctgcctgg cagtaaagga cacgaattct
    16501 ggaaacgaga ctttacaggt agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    16561 gtaatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    16621 ggggcgggta tgaatcgttg atcctggcga atcaaccaga acatatcgcc gccattcgcc
    16681 cacaaggcga gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg
    16741 tcgacgatct gattgccgat ctggacgccg gttttgcgcg aattgtataa cattgccact
    16801 tttggacaat tttgcagaca tttaattgtg aaaagtctta aattgttgcg tccgggatca
    16861 aggcgtcccg gacgaatcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    16921 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    16981 gcgctggcgg atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt
    17041 ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    17101 ggcgcattga ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    17161 gcagcaagtc tgggctgctg gctaagttat attcaggggc gctggttagg gaataccaaa
    17221 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    17281 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    17341 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    17401 tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg
    17461 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    17521 gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    17581 aacgcctgac gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg
    17641 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    17701 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat
    17761 gccgtcatgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    17821 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    17881 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag
    17941 cacgcggcta atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc
    18001 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    18061 cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc
    18121 tgataacgtg gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    18181 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    18241 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    18301 caggtattca ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc
    18361 ggaaaagaga attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc
    18421 gccatacaac aaacgcacat cgggcaacag ttctgacaaa ctattttctt taattttcag
    18481 tttattaact ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt
    18541 cttaggaata atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg
    18601 gaggaatagg caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca
    18661 atgaaaaaca tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt
    18721 aatgaacaac tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc
    18781 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag
    18841 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    18901 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    18961 ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    19021 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    19081 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    19141 agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa
    19201 gcaggcgttc cattctgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    19261 ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt
    19321 ggaacagtat gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    19381 tttgctgacg ctgatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    19441 gcgcgccaac gtcatgtggg gggcgacgca ggcgctgaac ggtttgattg gcgctggcgt
    19501 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    19561 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa
    19621 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgatga
    19681 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    19741 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    19801 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg
    19861 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    19921 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    19981 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    20041 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    20101 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    20161 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    20221 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    20281 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    20341 gaagcatgga aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg
    20401 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg
    20461 ataaaccaga tcgaacttca tccgctgatg caacaacgcc agctacacgc ctggaacgcg
    20521 acacacaaaa tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc
    20581 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    20641 atccgctggc atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt
    20701 attgccgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcgaaatt
    20761 gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacat
    20821 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    20881 cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    20941 tcatgcagag ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg
    21001 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    21061 cgtacacgca cagcccccct cactgaccgt agaacaaaca gtccggcata tttatcagaa
    21121 ctataaatca gatgccactg ccccttattt tggtgaaacc ggagagcggg cgataacttc
    21181 tgcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acgctgccgg gcaatattgg
    21241 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag
    21301 cgttgcgata acccaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21361 taaagatgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21421 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21481 tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21541 cgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    21601 ttaccgccag gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga
    21661 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21721 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21781 cattcatgtt cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca
    21841 gcggcgcgaa ggcaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    21901 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa
    21961 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    22021 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcagctg
    22081 gcgagcaatg tgcaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    22141 ctacgcccac aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat
    22201 gctcaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    22261 ccccggcagc atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg
    22321 tttcttcgca ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc
    22381 aggcgtctga cgctgggtcg ccatcggcgt atgtttcgtc aacgccggac gggtattgcg
    22441 gttctggcga cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg
    22501 acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca
    22561 gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgct tgtcgccctt
    22621 cggtacgaag acgtcttcac tcttataacc aatcttcgcc agcgggtttt tcccggtgta
    22681 atacatggtg gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg
    22741 gaaacgatgc tttttcagcc acagcgccag attcaccata tcttcatcac gcgtaccggg
    22801 gtgcgcggag atgaaatagg ggatcagata ctgctcttta cctgcctgtt ttgagtaagt
    22861 atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgataa
    22921 cggcccttct tcggtatgtt ccggggcaat cttcagataa ccgccgacgt gatgggtcgc
    22981 caactctttg atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat
    23041 gaggattttt ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc
    23101 gtggttagtg tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt
    23161 ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag
    23221 atcggaaatc acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat
    23281 gatcgaatct tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa
    23341 agaacagccg ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc
    23401 cggaatacgg gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac
    23461 gctgtccatc tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc
    23521 accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg
    23581 cgaagcatgg gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaacac
    23641 gtaggttttt tcccacggtt tcgggcgcgg tggctgcacg gttacggctt tggcttcctg
    23701 ctttttcggt gccaccggtt tgttatccgc gcacggcaaa tcttcaccat acggatgcgg
    23761 gattgggtcg atttttccag gggtatcaag acgggtggaa tccacgccgc tccagccagg
    23821 cagcgcctct ttcacgataa tcgcggtatt acgcacatcg cggatttcac taatcggctc
    23881 gcccatcgcc agacggtgcg ccacctccac cagcggacgc tcaccgttac caaacatcag
    23941 catgtcggct ttcgaatcca ccagcacgga acggcgcacg gtatcggacc agtaatcata
    24001 atgtgcggta cggcgcagac tagcctcaat accgccgagg atcaccggta catctttcca
    24061 cgcctcttta caacgctggg tataaaccag tgtggcgcga tccgggcgct tacccgcgac
    24121 gttatccggc gtgtaggcat cgtcatgacg taaacggcga tcggcggtat aacggttgat
    24181 catcgaatcc atgttgccag cagtaacacc gaaaaacaga ttcggtttac ccagacgcat
    24241 gaaatcgtct ttgctgctcc agtctggctg ggcgatgatc ccgacgcgaa agccctgtgc
    24301 ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc
    24361 agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat
    24421 cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc
    24481 gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata
    24541 atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg
    24601 ccctcctcgg gcgagtatga agattacggt accggattga ccaaaagttg ccctatcgag
    24661 ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa
    24721 ggctgaccga aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag
    24781 cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct
    24841 tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc
    24901 tgcgcggtga catcaatacg gttgacgtcc cacagttgtc cattaatacc cgggtcatca
    24961 cccagactga tatcgcgact gcgaatcggc gaaccggcca tgatttcagt tggcagcaag
    25021 cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc
    25081 agggtagaaa ccagaatgct ggatggctca aagaagccac gaatacgatc aacaatgctc
    25141 gccgcttcgc cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg
    25201 cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc
    25261 tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg ataacgacga
    25321 gagttcgacg cgttcagcag gcgcaagcgc acccagccac gcgagacttc aacgtacggg
    25381 ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg
    25441 tattctggcg taccaaagtt atccagccgt ttatcctgaa taatgaccgg aaaatcatcc
    25501 acaccataat ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt
    25561 ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac
    25621 agagtagctg cgttctggcg aataggcagt acgggtgccc agtcagcgtt tggcgacatc
    25681 atccgtgccg gaccgcccat cagcgggcct ggtacctgta gcccggctac cgtcattgag
    25741 acattttctg tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg
    25801 atagtcggcc ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta
    25861 aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagc
    25921 agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg
    25981 cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt
    26041 gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca
    26101 gattgccgac ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg
    26161 cgattttttg ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc
    26221 catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc
    26281 gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg
    26341 ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc
    26401 gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt
    26461 ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta
    26521 accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg
    26581 tcggcggttg cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga
    26641 tatagatagc attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc
    26701 cgaacagcgg cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac
    26761 gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg
    26821 taataataag acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt
    26881 gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt
    26941 gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt
    27001 tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgca agcgggagga
    27061 aacagcgccc tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga
    27121 gtcgatctca acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg
    27181 ttcgccagtg actttctgta gctcttccgg acgcagttta attttgcgtt tcccaacatg
    27241 aatggtcagc gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg
    27301 cgcggcttct gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg
    27361 cagatcgctt accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc
    27421 ggaagcatct tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa
    27481 agccttacct gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc
    27541 cgcatcggaa gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt
    27601 cgccccaggc ggcaacgtta atttgccggt gagcggctcg ccctgaccac gcgccgacgg
    27661 cagcgtaatc gggtcaatgg catagctacg accggtggaa tcaacaaaca ctaccggttg
    27721 gttgctctta cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg
    27781 cgcgtcgata tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt
    27841 gacaggttca gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg
    27901 caacggcgaa cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag
    27961 caggttattc attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc
    28021 cagttcactc tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt
    28081 cagttcgagg atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg
    28141 tttcggttca tcttcattac gaatgatctc aatcacttcg tcgatattga gaaacgccac
    28201 cagcaaacct tcgaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg
    28261 gcggcgcacg gtatcgcggc ggaacaccag ccattcggag aggatttcca gcaggttttt
    28321 caccgccgga cgaccatcca gaccgatcat attaaggtta atacgatagc tcttttccag
    28381 atcggtggta gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg
    28441 cacaatcacc aggcgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat
    28501 cggcagcttt ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac
    28561 ctgatgcggc aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat
    28621 acgcactgaa ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat
    28681 ttccgcttca gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt
    28741 tttcggctgg tcgattaatg cgattgccgc ctgagccact tcacgcaggt tatgcggtgg
    28801 aatatcggtc gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag
    28861 acgggcaggt agcattttcg gctcctgcaa agtgccgtcg aagtttggta cccagtcagc
    28921 cgtcccctgc cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt
    28981 gtaacgcatt gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc
    29041 aaccagcgga taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc
    29101 gctatcgccg tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt
    29161 aaatttggcg ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac
    29221 aggtttcaga ccatcaccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta
    29281 gtttaagtag gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat
    29341 taattctgat tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact
    29401 acattatctg ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc
    29461 cgacggcgat aaaggcatca atgtgccttg atatacaact ctttgctgta gacataatcc
    29521 tggggattat gcagcggaaa accgccaacg tagggtttaa gcagtttgat taacggctga
    29581 tagtagatag gaatcagcgg tgtctgttgg ttgatgatca cttctgcctg ctgatataac
    29641 gcattacgct ttgtcgcatc agtgatctgc gtggcctggt ttagtaaggc gtcatactgc
    29701 gcatttttcc agtgaccgac gttttcttca ctatcgcttt tgagcgtgtt caggaagctg
    29761 gaagcatcat tgtacgtcgc atcccacgac tgccgagaca gcatgaaatc accggctcgt
    29821 cgggcatcaa gataggtttt ccactccatt gtgcgcagcg tcacctgtgc acccagccat
    29881 tttttccatt cggaagacaa cgctatcgcg gtcttttcat gcagatcgta cttgttgtag
    29941 aacagctcaa agcgtagcgg atgagaggcg tcgtatcccg cctgtttcag caaggctttt
    30001 gccatcgcga cgcgctcact cattggcttt tgcagttcat cgaacgtcgt cgcgctaaag
    30061 ccttttacct ctggcggcgt cagcgtggtt gcgggcgttc tcaaccccag taccttttgc
    30121 gcaataagct gtcgatcaac cgtaagatat agcgcccgac gcactcgcac atcgttaaat
    30181 ggcggtttct caaggttgaa gttgtaatat tcgctgttca gacgcggaat aattcgtagc
    30241 tcgccaggca gtgatttttc aatggcggga atttgctgcg ccggaaccca ggtgagatcg
    30301 acctctcccg cgcgatagcg gttatagccg gtgaccgaat tatctagcgc cagatactca
    30361 acctgttgca atactgtatg ttgcgcatcg cggtactttg gatttttgcg tgcagtaatc
    30421 ttttcgttaa ctacccactg ttcaagcaca aaggcaccgt tgtaaaccat gttctctggc
    30481 ttactccagc tatcgccatg tttagcgatg acatgatgag gaaccgggaa cagcgtcggc
    30541 caggcgagca tcgtcgtgaa ccaaggaacc ggctgctcaa gcgtaacttc aagagtacga
    30601 tcatccgtcg ctttgacacc cagcgatgta acatctgctt tacccgcaac aatagctgcg
    30661 gcattgttaa tgtgcgcctg tgccagatag ccagcaaaag ggcttgccgt tttcgggtca
    30721 accgcgcgct gccagccgag gacaaaatcc tctgccgtca gaggctgacc gtcggaccac
    30781 tgcaaaccgc tacgcagatg gaaaatatag cgcttgccgc cgtccagtat ctcccagcgt
    30841 tcagcctgag cgggctgcac ctggccttca ccgtccatcc ataccagacc ttcaaacaga
    30901 tccagcacaa tctgcgcggc agtattctcc tccacctttt gcgggtcgag cgtacctggg
    30961 tcgctatgat tgttgtaacg aaagacttgt tgcggggcga gcggtgtgtt ggcgggaacg
    31021 tcagcagcgt agagaggagc cgcacttacc aggctgacca gccataataa atttcgcgta
    31081 tacatggtat tccttaccgg agaaattata agcccaacaa caatacgcct gtggcattgt
    31141 tcgctcaaac ttatcgcgag tgatttggct cacactccgg taaagaaaaa agcgagttgc
    31201 ccccgctttt ccattaatta acggatttca ttcaatagtt ctggatgctt atccagaaca
    31261 cgtaaaagtt tgattgtgga aggatgaggt tgggcattgc ctttttcgta acgcgaaaac
    31321 gcatttacac ctcccccaaa aatttcgctt gcctcttttt gggtaagaga gagctttttt
    31381 cgaaccttca ctataaattc aggtgccact gtttcggcat tcaccgaagc ccgaaatgcc
    31441 tttacttgcg ccatgaaagc atctgactct tctttattca tgatgctctc ttcgcaatgg
    31501 acacaatata aaccgtggat acctttcaat actgtttttc gtccacggaa ggtgtatgga
    31561 atatctttaa tgccagaaac catttctccc tggtggcaaa ccggacattt catattactt
    31621 ctccttaaac gagacgatca gtacgtcatg aattaccgta attttaagat aaacctggcc
    31681 tgtaacaagc ctgggtctgt aaacatcctg ccagatagta tgatcagagt aggtggtcat
    31741 gcttttataa aagtcgctct ctgataatcc aatgataaca ttacacatac catcaaaatc
    31801 caaacctaac tcatctgcat ttaacagtgc actacgtgtt gtacgaactt gcccggcatt
    31861 gacaagtttt ttaacctgac tcaaacgtgt atgtggtgtg cgtttttcca taacccccgc
    31921 ctccctgtta ctttagttat aacctaaaag gttaattaca gcaatgaaaa accacctaaa
    31981 aggttagtta gatgtacgga tatagtgacc acacaaaacg tattctttaa ggaaagtgat
    32041 tgaccatata agaaagtggc gcattagtag cgccagtttg aagcaggaat ttataaggga
    32101 agctggagtc aggcatcacg ctaacggcac ataaacatcc gtttccagtc gctgctctgt
    32161 caccccttcg gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc
    32221 gcttgcaggc agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc
    32281 gccaacgtgg cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata
    32341 acgattatcg ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg
    32401 tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga
    32461 aagccccgtc tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg
    32521 gctacagcgg tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc
    32581 cagtgtcagg tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga
    32641 gcagtaacaa ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc
    32701 agtctttatc gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacgccgt
    32761 tccagcgttt gtgatcgata tcaacattga tagtaccgct ggcatctttg aacacgtaga
    32821 gatcgtcaga gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca
    32881 gggattttgc gctttctaca gtcgttacgc tgccgttcgg cccctggaat cctccggcct
    32941 gactttgcgt tgccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcgc
    33001 tgcacagggc cattactgcg attactgctg cgaatttttt catgtttatt actcccttta
    33061 atgtctgttt ccgagcattt aacaagatag tccttaacaa cttcttaagg gaaaaaaata
    33121 aaatttagtg ctgtacagag cgcgttacaa cacggtttac tggcagcaaa tacggttatc
    33181 gcagggatga aaaaatgcga attttactga tagaagatga catgctgatt ggcgacggca
    33241 tcaaaacggg ccttagtaaa atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg
    33301 gaaaagaggc gctttatagc gcaccttatg atgcggtgat cctggattta accttaccag
    33361 gcatggatgg tcgcgatatt ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac
    33421 tgatcctgac cgcgcgcgat gcgctggcgg aacgtgtaga agggctgcgt ctgggagctg
    33481 acgattatct gtgtaaacct tttgcgttga tagaagtcgc cgccaggctg gaagctctga
    33541 tgcgccgaac caacggccag gccagcaacg agctgcgcca cggtaacgtc atgctcgacc
    33601 ccgacaaacg tatcgccacg ctggctggcg aacccttaac actgaaacca aaagaatttg
    33661 ccctgctgga attactgatg cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag
    33721 agaaactgta tacctgggac gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc
    33781 atctgcgacg caaactcggt agtgatttta ttcgtaccgt gcatggtatt ggctacacat
    33841 taggtgagaa atgaaattta cccaacgtct tagtctgcgc gtcaggctga cgctaatctt
    33901 tttaattctg gcctcggtga cctggctgct ttccagcttt gtcgcctgga aacaaacaac
    33961 ggataacgtc gatgaattgt tcgacaccca actgatgctg tttgccaagc ggttaagtac
    34021 gctcgatctc aacgaaatca acgcggcgga tcgcatggca cagacgccaa atagattaaa
    34081 acacggtcat gttgatgacg atgcgctgac ctttgccatc tttacccacg acggcagaat
    34141 ggtccttaat gatggcgata acggagaaga tattccctat agctatcaac gggaaggttt
    34201 tgctgacggg caactggtcg gtgaagacga tccttggcgt tttgtctgga tgacctcacc
    34261 tgatggcaaa tatcgcatcg ttgttggcca ggaatgggaa taccgtgaag acatggcgct
    34321 ggcgattgtt gccgggcaat tgatcccgtg gctggtcgca ctgccgatta tgttaatcat
    34381 catgatggta ctactgggtc gtgaactcgc gccgctgaac aaactggcgc tggcactacg
    34441 tatgcgtgac cctgactcgg aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg
    34501 gccactggtt gagtcgctaa atcaactgtt cgcccgcaca catgcgatga tggttcgtga
    34561 acgacgcttt acctccgacg cagctcacga acttcgtagc ccgttaacgg cgctgaaagt
    34621 gcaaaccgaa gttgcgcagc tctctgacga tgatccgcag gcgcggaaaa aagcactgct
    34681 tcaattacat tccgggatcg atcgcgctac tcgtctggtt gatcaactgc tcacgctatc
    34741 gcggctggac tcactggata accttcagga cgtcgcggag atcccgcttg aagatctcct
    34801 gcaatcgtcg gtgatggata tttaccacac ggcgcagcag gcgaaaattg acgtgcgact
    34861 gacactcaat gtccagggca tcaaacgcac cggacaaccg ctattgctaa gtttgttggt
    34921 gcgaaatttg ctggataacg ccgtgcgtta cagtccacag ggcagcgtgg tggacgtcac
    34981 gctgaatgcc gataatttca tcgtgaggga taacggcccc ggtgtgacac cagaggcact
    35041 ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa accgccaccg gcagtggact
    35101 tgggctatcg attgtccagc gaatcgccaa gctgcatggc atgaatgttg agtttgggaa
    35161 tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa gggtaagact tttgctaaat
    35221 tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc gctctgtgac gtactatagt
    35281 cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat cctgattatc aacggcgcga
    35341 aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc gcggatggca
    35401 cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac tacgatgtca
    35461 aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg ccaggctggt
    35521 ggatgggcgc gccgtggaca gtgaaaaaat acattgatga tgtattcacc gaaggtcacg
    35581 ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc gtcgaaaaaa tacggctccg
    35641 gcggcctggt acagggcaaa aaatatatgc tttctctgac ctggaacgca ccaatggaag
    35701 ccttcaccga aaaagatcag ttcttccacg gcgttggcgt tgacggcgtg tatctgccgt
    35761 tccataaagc aaaccaattc ctcggtatgg aaccgctgcc gacatttatc gctaatgacg
    35821 tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat cttgtggaaa
    35881 tttttggtta actagagctc aggctttaga aggagttaac catgcttacc gtaatcgcag
    35941 aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag tttgctaaaa
    36001 tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg gtggattgcg
    36061 ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgata gagcagtggg
    36121 aaagcatcgc gcatcttgaa gcgcatctgc aaaccccgca catgaaggcg tatagcgaag
    36181 ccgtaaaagg tgacgtgctg gagatgaata ttcgtattct gcagccaggg atttaacatt
    36241 cctgaaaaga ggccggtgct gtttgccggc catttatatt cactcttccg ccgtctgtaa
    36301 ccgtgcttta atctgttcgt aaaattcatt attaatcaga taaaatcgca tgatgagtcc
    36361 ggtaatcagg ttaagtatcg cgggaaagat cgtcattaac aggcagatcc cccacagcgc
    36421 ctgtggtgtt tgttctacac caggttgata atccaaatag gctaaagcga ttccgataaa
    36481 gccccctgcc agccccatac cgaacttttg gaaaaagaga atcccgccaa atgccaggcc
    36541 agaaacacgt tgccccgttt tcatttcacc gtaatccacg gactcagcaa tggatgccca
    36601 ataaatggga agctgaatat ccgtcaggaa ggtcaccaca atataaaatg taaaagctaa
    36661 cactatagaa tttggttgca cgaaatacat cgcaccaccc accacgaacg ccagtaattg
    36721 ggtataacga aatattttta ttttgtcgta gagtcgagta agccaggtac aagcgatcat
    36781 agccagaaca gaggcgatca cgcctgaagt taaaaatggt gaaattaaag catccccacc
    36841 attcaggtaa tattttgcat aatatgcagc gacagagtta cgaataatcc cgccaaacat
    36901 aatgatcgca attacagcgc ccaaaatgag ccattgatca tttttgatca gtaaccgaaa
    36961 ctgcgcggcc ataccgagac tgggttttgg atggtagatg cgctctctca cgctaaaaaa
    37021 acaatacaga cataaacacg ttgccagaat tcccattgcc cccattgcta tttgataacc
    37081 cagttttacg ttattcttcc caagatacat tgcagataaa ggaacaataa ttgttacggc
    37141 gaacatggca atttttgtca taacaaaacg ataaccattg gcgctcaatc gctcttgtgg
    37201 atcatccgtg ataacgctga ttaatgaaac ataaggaata gcgattaacg tataggtcaa
    37261 ggtaagtaac acataggtag cccaggccca taaaagctta actgaataat cggtatcagg
    37321 tgtggtgaac atcaaccaaa tgcttatccc aaacggtatt gacataaaaa gcaaccacgg
    37381 ccgatatttt ccccaacgtg tattcgttct gtctgtcatt gttcccacaa ctggatcaat
    37441 cacagcatca aggatacgta cagaaaataa cagaatacct aagtcgacgg gtttcagacc
    37501 aaatacatcc gtgtaaaaat aagtgatgat catggctaaa gacatcatta ctatcgcaat
    37561 agccatatca ccggcaccaa aacctatctt ttcatttatt gtcagtttca tacacagcac
    37621 tcattgatca cgtgaaatta tattaaaaac aaatgtatcg gaactcattc agcacataac
    37681 tttacgagtg agaataaatg cattggtaga taccacaaca aaccactgag ttaatattga
    37741 gcagcatcac ataaaaacca atctcattta acttaatgat ttttaataat aaatgtggtt
    37801 ttttctgttt tatatcacag ataacaaaga agcatactgc gcaataaaaa taaaagaatg
    37861 tagagttggt atcaaccact tgaaatgagg ctttgtcatg ataagaattt ataaagccag
    37921 taaaggtcag cttaagcatc gtgaaatttt tgatgaaata aagaaaaaga ttgataacgg
    37981 tgaatggaaa gaaggcaaag ctataccaac agaaagagaa cttgctgaac attttttaag
    38041 cagcagaaca acaattcgga aagcaataga aagtttgaaa caacgcggat acctgcatag
    38101 tgtccacggg caaggaacat ttacattacc ggctcgcagt cgggaaaatc atttattaca
    38161 tagttttact gatgacatta aggctcgtgg cggcgtacct gcacagaata ttcttgaaat
    38221 aggttatatt cctctaagtg atattatcag aaaaaatctc gaacttgata tccatgtcca
    38281 taccgttttc tgtataaaac gcattcgcta tatgggaagt acgccgctgg gtattcagac
    38341 atcatggctt gcgttaaatg acggacaaga aataactcag gaagagctgc tggcgacggg
    38401 ttccctctat attttactgg aagaaaaact cgctattaaa cctttagaag caacggaaat
    38461 tatctctgcc cgacttccct cgcctcttga gcgtcaatta ctggaactga ctgccgatga
    38521 cgtcgtatta agttgtaccc gcgtcacgtt atccgtggaa cgcaaaccaa tggagtatgt
    38581 agagatggtt tatcccgcaa gtcggtactc ctacaaagca cgaattacaa aagacagttt
    38641 tagcgtgtaa cagagagaat attgtgaaca aaatagaaca agccgtaatt tataacaaaa
    38701 tattaggttc tttagcctgc gcgggtatgg gcgatgcatt aggcgcagcg actgaactct
    38761 acagcattga tgaaataaaa gcgcaatggg gaggtttttt aaatgccttt gtttctcctc
    38821 ctgccgatac ctttgctggc tcattaaatg gtattgcggg acttattacc gacgattcca
    38881 gccaaatgta tgtattttca gaagggttag ttgaggctgg atttgataac ttcaccaata
    38941 atgactggtt agcttgtctt ctgcgttggg ccgatatgca gccctacgca aattataaag
    39001 gccccacaac agaacaaatc gtcaaggcac taaaagaagg tagaccaaca aacaccattg
    39061 ggcgtattgg aacatcttct cgtcaggcac ccaatgtcgg caccacgaac ggcgcaggaa
    39121 tgcgtgtagc cccggcagga ttgatctggc caggcaagaa agaaaaagcg tgtcacctgg
    39181 cgctaataac ttgcctacct tcgcatgaca ccaatattgc gattgccagc gcttgcgcca
    39241 tcgctgcggc gacatcacaa gctatgttac cagaagcctc gctcacatca ttactggacg
    39301 ccgcaatctg gggagcgaat tacggcgaac gcctggccaa acaatatgca cgatgtgtcg
    39361 cagggccatc tatcgccatg cgcatccaac ttgcagcgga catcgccaga cgagccaatg
    39421 acctggagtc ttgtctgcga gaaatggaag gtttggttgg taactctgtt gccgcccatg
    39481 aatccattcc tgcggcaatc ggtctattgc tgtactgcaa aggagaaccc tgggaaacca
    39541 ttcatgcctg cgccaatatt ggcaatgaca ccgatagcat cgctacgatg gcaggcgcaa
    39601 ttgcaggggc atggcgcggt tttgatgcgc tccctgaaga taaatatgcc tttttccgcg
    39661 ccgtaaataa taaagatttc gatattgaag cgatcgcatc gggtctgaca ttgcttgcat
    39721 tacaggcaca ggagaaataa tgaacatgcc agcccgtact cctgttatgc cattagcgca
    39781 aactcgtgag gaaataaatg ctacggcggc atcgttacgc catttggcag ctatctgggg
    39841 tgaatgtttt tctacttttc agctcgataa acgtgctgtt gaacgcgtct gtatagtggg
    39901 atcgggcgac tcctggacgg tggcactatg tgccgccgcc tggctgggga aatacacaca
    39961 tctgttttgt tacgcattgc aaacgtggga ttttcttcag actgatttaa cccgttatca
    40021 gaaagaaacg ttaatcatta ttctcagtgc aagtggcaga ccatcaatga ccgttgacgc
    40081 cctctgccac gcagtatgct caaacgcgca agtgctgggt gtgacgaatt tccctggaac
    40141 accattttgc gcaataacag aaaacatgct ctatacatgg gcgaataagc agggtattcc
    40201 aacgcaaagt agtagcgtca cactttatgc cctcctgcgc ttagcgcaaa aattatgtcc
    40261 aggcttaaca ccactgcaga ttgaagagga tttggaaggg aaatttagcc agattaatca
    40321 gcactggcag caaaagaagc gccattttta ccaacaaaaa gagataacgt ttcttgggag
    40381 tggcttaagt tggggactgg caatatcggg gagcaattta ctttcttgcg gcccacagat
    40441 tcgtgcgaca gcccttccac tggaagaatt ttatcattca ttacgcttac atcaggctgg
    40501 tgtcgggcag cattatattt tactaccagc cacatcctgc gagtccccat tttaccttgc
    40561 aacccaaaaa gcaattgttg aacagggagc gacagcagaa ttaatcagtt ttatacctga
    40621 cgccagtgag tctaacaatt tatttctggt catgcagtgg ttatatgaaa tgtgctggca
    40681 tttaagttgt gattatgtcg acgcaggcgg gcaacgcgtt agccataggg agaaataaga
    40741 ctatgaagaa atttgatgtc gccgctttag gcagtggaaa tatagatatt tttttatcta
    40801 tcccgtcact ccccactcgt ggcggtaaag ttgtcgggac acgtctgggt gaacaagctg
    40861 gcggcacagt cgccaacagt gcctgtgcga tgggacaact gggtctcaat gtggtatctg
    40921 tttcatgtgt gggcaatgat cattcagcct cgatcattct tgatggattt aaaaaatatc
    40981 atgtaaattg tgatttcgtt caggtaattc cggaattgat tgccaatacc gcgattatct
    41041 ttattgatga attaggtgaa aaaacgttgg tttactcgcc tggtagtgat catgagtggg
    41101 ataaagaaaa agccctgcag gcaattgcgc aaagccgtta tttttatacg atgccagcag
    41161 atatcgagaa atttcgtatg ctggccgaat attcccgtag ccaaatgaca aaagttgtcg
    41221 ttgatattga gcctcatatc attgcaacac ctgaacacct tgccagaatt ttacaccttg
    41281 ctgatattgc gatatttaat tatgacggtt ttattcgtgg ctatgcaaca gagccagatt
    41341 ttacacttct gcatgatata caagacgagt atcaacttga cgctgtcgtg gttacgctgg
    41401 atgttagggg agtaattgca gtcaaaggca atgagcaagt aaaaataggt agttatacaa
    41461 ttcccgttat tgataccaca ggcgctggag atacttttaa tggtgcattt gtttatagct
    41521 taataaaaaa catgccctta attgaagcac taaaatttgc ttctgccaca gcagcaataa
    41581 atattactgc cttaggtgcc agaggtcatc tgccgacacc atcggaggtt aatctttttc
    41641 tttcacaaca cgactaaaat catatatata caaaggtaaa tatcatgaaa ataagatatg
    41701 ccgcta
//
LOCUS       DAPHJE010000029        41148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPHJE010000029 DAPHJE010000000
VERSION     DAPHJE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.029"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            23..763
                     /locus_tag="RH222_001557"
     CDS             23..763
                     /locus_tag="RH222_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796605.1"
                     /translation="MEKLLDAYKRILQEVNAQSFNLNEDKYSGVFLPVPFEEYWHSPI
                     KIMLVGRETAGWNTLDGKNTISRVLGLIPDVTIGQVVEEAVDRYRKHLPVQNYGTANL
                     KSRSRFTQYHFRLARELNIPPQAIVYANLLAWDYDGLTPLNRPQNEVQEVILASLKLL
                     AVQIKHLEPDFIIFASGARRTDYIIKQVLTELGGYETSSVIPGKLWEFKTGNTICFRI
                     AHPRAMRGHQKYRDEVIARIKQLCTKGG"
     gene            complement(1005..1091)
                     /locus_tag="RH222_001558"
     tRNA            complement(1005..1091)
                     /locus_tag="RH222_001558"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1055..1057),aa:Leu,seq:taa)
     gene            complement(1104..1177)
                     /locus_tag="RH222_001559"
     tRNA            complement(1104..1177)
                     /locus_tag="RH222_001559"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1143..1145),aa:Cys,seq:gca)
     gene            complement(1232..1307)
                     /locus_tag="RH222_001560"
     tRNA            complement(1232..1307)
                     /locus_tag="RH222_001560"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Gly,seq:gcc)
     gene            complement(1459..2007)
                     /gene="pgsA"
                     /locus_tag="RH222_001561"
     CDS             complement(1459..2007)
                     /gene="pgsA"
                     /locus_tag="RH222_001561"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV3796606.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(2064..3896)
                     /gene="uvrC"
                     /locus_tag="RH222_001562"
     CDS             complement(2064..3896)
                     /gene="uvrC"
                     /locus_tag="RH222_001562"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV3796607.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3893..4549)
                     /gene="uvrY"
                     /locus_tag="RH222_001563"
     CDS             complement(3893..4549)
                     /gene="uvrY"
                     /locus_tag="RH222_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV3796608.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4845..5021
                     /locus_tag="RH222_001564"
     CDS             4845..5021
                     /locus_tag="RH222_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796609.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            5008..5232
                     /locus_tag="RH222_001565"
     CDS             5008..5232
                     /locus_tag="RH222_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV3796610.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(5300..6022)
                     /gene="sdiA"
                     /locus_tag="RH222_001566"
     CDS             complement(5300..6022)
                     /gene="sdiA"
                     /locus_tag="RH222_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV3796611.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(6252..7004)
                     /gene="yecC"
                     /locus_tag="RH222_001567"
     CDS             complement(6252..7004)
                     /gene="yecC"
                     /locus_tag="RH222_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV3796612.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(7001..7669)
                     /gene="tcyL"
                     /locus_tag="RH222_001568"
     CDS             complement(7001..7669)
                     /gene="tcyL"
                     /locus_tag="RH222_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV3796613.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(7684..8670)
                     /gene="dcyD"
                     /locus_tag="RH222_001569"
     CDS             complement(7684..8670)
                     /gene="dcyD"
                     /locus_tag="RH222_001569"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV3796614.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(8775..9575)
                     /gene="tcyJ"
                     /locus_tag="RH222_001570"
     CDS             complement(8775..9575)
                     /gene="tcyJ"
                     /locus_tag="RH222_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3796615.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(9663..10214)
                     /gene="fliZ"
                     /locus_tag="RH222_001571"
     CDS             complement(9663..10214)
                     /gene="fliZ"
                     /locus_tag="RH222_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV3796616.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLEQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVKKSSSLPSSDIY"
     gene            complement(10260..10979)
                     /gene="fliA"
                     /locus_tag="RH222_001572"
     CDS             complement(10260..10979)
                     /gene="fliA"
                     /locus_tag="RH222_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV3796617.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTTFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(11299..13140)
                     /locus_tag="RH222_001573"
     CDS             complement(11299..13140)
                     /locus_tag="RH222_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDV3796618.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATTDNLTLAGFTAGTKAADGTVTYSKNVQFAAA
                     TASNVLAAAKDGDEITFAGNNGTGIAATGGTYTYHKDSNSYSFSATAASKDSLLSTLA
                     PNAGDTFTAKVTIGSKSQEVNVSKDGTITSSDGKALYLDEKGNLTQTGSGTTKAATWD
                     NLMANTDTTGKDAYGNSAAAAVGTVIEAKGMTITSAGGNAQVLKDAAYNAAYATSITT
                     GTPGDAGAAGAAATAGNAAVGALGATAVDNTTADVADISISASQMASILQDKDFTLSD
                     GSDTYNVTSNAVTINGKAANIDDSGAITDQTSKVVNYFAHTNGSVTNDTGSTIYATED
                     GSLTTDAATKAETTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSE
                     AQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            13406..14812
                     /gene="fliD"
                     /locus_tag="RH222_001574"
     CDS             13406..14812
                     /gene="fliD"
                     /locus_tag="RH222_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV3796619.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQIARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            14837..15247
                     /gene="fliS"
                     /locus_tag="RH222_001575"
     CDS             14837..15247
                     /gene="fliS"
                     /locus_tag="RH222_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV3796620.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            15247..15612
                     /gene="fliT"
                     /locus_tag="RH222_001576"
     CDS             15247..15612
                     /gene="fliT"
                     /locus_tag="RH222_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV3796621.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            15690..17177
                     /gene="amyA"
                     /locus_tag="RH222_001577"
     CDS             15690..17177
                     /gene="amyA"
                     /locus_tag="RH222_001577"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV3796622.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(17211..17624)
                     /locus_tag="RH222_001578"
     CDS             complement(17211..17624)
                     /locus_tag="RH222_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3796623.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            17811..19016
                     /gene="yedE"
                     /locus_tag="RH222_001579"
     CDS             17811..19016
                     /gene="yedE"
                     /locus_tag="RH222_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV3796624.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            19013..19246
                     /gene="yedF"
                     /locus_tag="RH222_001580"
     CDS             19013..19246
                     /gene="yedF"
                     /locus_tag="RH222_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV3796625.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            19355..20023
                     /locus_tag="RH222_001581"
     CDS             19355..20023
                     /locus_tag="RH222_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV3796626.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(20416..20730)
                     /gene="fliE"
                     /locus_tag="RH222_001582"
     CDS             complement(20416..20730)
                     /gene="fliE"
                     /locus_tag="RH222_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV3796627.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            20945..22603
                     /gene="fliF"
                     /locus_tag="RH222_001583"
     CDS             20945..22603
                     /gene="fliF"
                     /locus_tag="RH222_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV3796628.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKAMQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            22596..23591
                     /gene="fliG"
                     /locus_tag="RH222_001584"
     CDS             22596..23591
                     /gene="fliG"
                     /locus_tag="RH222_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV3796629.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            23584..24270
                     /gene="fliH"
                     /locus_tag="RH222_001585"
     CDS             23584..24270
                     /gene="fliH"
                     /locus_tag="RH222_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV3796630.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLI
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            24270..25643
                     /gene="fliI"
                     /locus_tag="RH222_001586"
     CDS             24270..25643
                     /gene="fliI"
                     /locus_tag="RH222_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV3796631.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            25662..26105
                     /gene="fliJ"
                     /locus_tag="RH222_001587"
     CDS             25662..26105
                     /gene="fliJ"
                     /locus_tag="RH222_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV3796632.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            26102..27229
                     /gene="fliK"
                     /locus_tag="RH222_001588"
     CDS             26102..27229
                     /gene="fliK"
                     /locus_tag="RH222_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV3796633.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPDFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPASLQGRVTGNSGVDIFA"
     gene            27334..27798
                     /gene="fliL"
                     /locus_tag="RH222_001589"
     CDS             27334..27798
                     /gene="fliL"
                     /locus_tag="RH222_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV3796634.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            27803..28807
                     /gene="fliM"
                     /locus_tag="RH222_001590"
     CDS             27803..28807
                     /gene="fliM"
                     /locus_tag="RH222_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV3796635.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            28804..29217
                     /gene="fliN"
                     /locus_tag="RH222_001591"
     CDS             28804..29217
                     /gene="fliN"
                     /locus_tag="RH222_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV3796636.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            29220..29585
                     /gene="fliO"
                     /locus_tag="RH222_001592"
     CDS             29220..29585
                     /gene="fliO"
                     /locus_tag="RH222_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV3796637.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            29585..30322
                     /gene="fliP"
                     /locus_tag="RH222_001593"
     CDS             29585..30322
                     /gene="fliP"
                     /locus_tag="RH222_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV3796638.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPIRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            30332..30601
                     /gene="fliQ"
                     /locus_tag="RH222_001594"
     CDS             30332..30601
                     /gene="fliQ"
                     /locus_tag="RH222_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV3796639.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            30610..31395
                     /gene="fliR"
                     /locus_tag="RH222_001595"
     CDS             30610..31395
                     /gene="fliR"
                     /locus_tag="RH222_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV3796640.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            31685..32308
                     /gene="rcsA"
                     /locus_tag="RH222_001596"
     CDS             31685..32308
                     /gene="rcsA"
                     /locus_tag="RH222_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV3796641.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(32352..32540)
                     /gene="dsrB"
                     /locus_tag="RH222_001597"
     CDS             complement(32352..32540)
                     /gene="dsrB"
                     /locus_tag="RH222_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV3796642.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            32703..32930
                     /gene="yodD"
                     /locus_tag="RH222_001598"
     CDS             32703..32930
                     /gene="yodD"
                     /locus_tag="RH222_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV3796643.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            33228..34043
                     /locus_tag="RH222_001599"
     CDS             33228..34043
                     /locus_tag="RH222_001599"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV3796644.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(34040..35734)
                     /gene="yedQ"
                     /locus_tag="RH222_001600"
     CDS             complement(34040..35734)
                     /gene="yedQ"
                     /locus_tag="RH222_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDV3796645.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKKLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(35972..36154)
                     /locus_tag="RH222_001601"
     CDS             complement(35972..36154)
                     /locus_tag="RH222_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV3796646.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(36233..37150)
                     /locus_tag="RH222_001602"
     CDS             complement(36233..37150)
                     /locus_tag="RH222_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV3796647.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            37323..38243
                     /gene="yedA"
                     /locus_tag="RH222_001603"
     CDS             37323..38243
                     /gene="yedA"
                     /locus_tag="RH222_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV3796648.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLMRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(38232..38702)
                     /gene="vsr"
                     /locus_tag="RH222_001604"
     CDS             complement(38232..38702)
                     /gene="vsr"
                     /locus_tag="RH222_001604"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV3796649.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(38683..40101)
                     /gene="dcm"
                     /locus_tag="RH222_001605"
     CDS             complement(38683..40101)
                     /gene="dcm"
                     /locus_tag="RH222_001605"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV3796650.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(40168..40605)
                     /locus_tag="RH222_001606"
                     /pseudo
     CDS             complement(40168..40605)
                     /locus_tag="RH222_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
     gene            40661..41148
                     /locus_tag="RH222_001607"
                     /pseudo
     CDS             40661..41148
                     /locus_tag="RH222_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT        10027 a        10764 c        10392 g         9965 t
ORIGIN      
        1 tattacggga taggattgaa gaatggaaaa gctattagac gcgtacaaac ggatattgca
       61 ggaagttaac gcccagtcat ttaacctgaa tgaggataaa tactcaggcg tatttttacc
      121 cgtacccttt gaagaatact ggcattcacc gataaaaatc atgctggttg gacgtgaaac
      181 cgcaggctgg aatacgctgg atggtaagaa tacgatatca cgggtgttgg gacttatacc
      241 tgacgtcacc atcgggcaag tggttgaaga agctgtagat cgttacagga aacatcttcc
      301 ggtacaaaat tatggcaccg ccaacctcaa atcacgtagc cgcttcactc aatatcattt
      361 caggctggcc cgggagctta atatccctcc tcaggctatc gtgtatgcca atctactggc
      421 gtgggattac gacggactga cccctctcaa tcgccctcaa aatgaagtgc aggaagtcat
      481 attagcctcg ctgaagctac tggcggtaca aattaaacac cttgagcctg atttcatcat
      541 ctttgcatct ggagcccgga gaacagacta catcataaaa caggtgctta ctgagctagg
      601 cggctatgag acttcttcag tcattccggg gaagctgtgg gagttcaaaa caggtaatac
      661 aatctgtttc agaattgccc atccaagggc tatgcgcgga caccagaagt acagagatga
      721 ggtgattgca cgaattaagc aactttgtac taagggcggc tagagcatta caacttgctc
      781 agcaggccac ccttcccgga agagtaccgg aaaacaggca aagcggcctg gttctctatg
      841 ctggaggcga cacgttcgat tcggcattgc caggctggag attgcggctc attgcacccc
      901 gtgagcacag gatatgcaca gggcgtgaat ttcaggcaca aaaaaaccac ccgtaggtgg
      961 tttcacgaca ctgcttattg ctttgattat tcttgtcttt cccatggtac ccggagcggg
     1021 acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc
     1081 accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga
     1141 tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca
     1201 gacatccgct tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg
     1261 accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa
     1321 tcagttaatc acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc
     1381 attctactta cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg
     1441 aaaattacgc cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg
     1501 caacattgac cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc
     1561 aacccaaatg ttcggacgcc acagcagcca tgccaacgcc accatctggg cagtggtttt
     1621 cacttttcca atccaggaga cggccacgct actgcgttta cccaactccg ccatccattc
     1681 gcgtagcgca gaaataataa tttcacgggc gatcatcgtt gccgccggta atgtcaccca
     1741 ccagctgtga taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc
     1801 cacagggtca aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc
     1861 atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca
     1921 ggtgacaggc agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt
     1981 aagcaacgta gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt
     2041 acccctatgt tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga
     2101 ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc
     2161 aaaccgccca tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt
     2221 tccagggaac tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga
     2281 tcatgtgatt catcgcgaat atgctggata acatgcagcg cgggtgaatc tggcggcaaa
     2341 ctaaatccct caccttccgg ctcaaagaac agcgtttcca gtccagcctt acgatctgct
     2401 cctttggcaa cgccaagtag cagcggatga tttttatccc atgagacatc cagttcggcg
     2461 aagacatttt tcgcctgcgc aagctggcct ttgccgccgt cgataaggat cacatccggg
     2521 atcttactgt cgtcaatggc tttaccataa cgccgacgca gcacctgatt catcgccgca
     2581 taatcatcgc ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc acgcagcggg
     2641 ccgttagcat caaacaccac acaggaagcg acggtttgtt cgcccatggt atggctgatg
     2701 tcaaagcact ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt
     2761 cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg
     2821 cgcgcgagtt tcagataacg cgctctatcg ccgcgaggtt tggtttgaac attaatcttg
     2881 cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg
     2941 agcaggatct cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg
     3001 aaggtttcta ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg
     3061 ctgccgagca ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg
     3121 aacgccacac caataacgtc gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg
     3181 acgcgtcgca ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa ctccagattc
     3241 tggctggcag tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac
     3301 aaaaacaggc gcacatactc gacctgctga gcgtattctt cttcactcac cagtccttca
     3361 acgcacggtc ccagacagcg ccctatctgg tattgcagac acggacgcga gcgattgcga
     3421 taaacactat tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca
     3481 cgtacggcat agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca
     3541 cgatgcatcg ccagacgcgg gtgggtatca ccactcagga agataaaagg atatgattta
     3601 tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc
     3661 aacagcgctt cggtttctgt gtgagtaacc gttacatcaa tttgctggat ctgggcgacc
     3721 agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat agctggaaag ccgttttttc
     3781 aggtctttcg ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg
     3841 cctggctggc tggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac
     3901 ttgataatgt ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat
     3961 cgccatgaat gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac
     4021 tgagattgag ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa
     4081 tctgcaattc acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct
     4141 ggcttaacgc catttgttga gcgatgtcag aagcaatgta acgctgccct gaatagacag
     4201 aacgaatcgc actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac
     4261 cggcctgcat gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt
     4321 tgacatcagc tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca
     4381 tactcatgtc cattagcacc acgtcaacgg catttgtccg gcaccactta acggcgtctt
     4441 caccgcacga tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta
     4501 tccctgcgcg caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc
     4561 cagaaatagg gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca
     4621 actttgaaac gttaaaaatg gtgggtattc gatttttctc tcgtttttgt caattcgatt
     4681 gtccacagtt atggtcacgc ccgtcaccaa cggcttaccg gatcgtttca cagtaagccc
     4741 tgcgacgaaa tgtgacaaaa attaccttta ttcagcaaaa atgaaaatca gcccgctgaa
     4801 tatgtaacat taattaaccc gaacaatacg ggcaaaaaac attcatgata aaaatattta
     4861 ttggtcatta tattaacgca tttaatagca ccgttgacga tacgctcaaa aaacaaccac
     4921 tgctattttt agcaaagctt atggtatact ccgccgcctt aacatttttc accgcaaatt
     4981 ttcattgcaa catgacgagg aaaataaatg agtacgcctg atttttctac tgccgagaat
     5041 aatcaagaac tggcaaatga agtctcctgc ctgaaagcga tgctgacgct gatgctgcag
     5101 gcgatgggac aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg
     5161 atcgaagacg aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc
     5221 taccgtcagt aatataaaac cggctgatag cgtgcctttc agccggtttt tgcatctggc
     5281 acgcaggaca gaaaagagat caaattaagc cagtagcggc cgcgtaacag gcaacctggg
     5341 tcttattcgg tgcattgatt tttttctgca tgtttttctg atggaaattg accgtattct
     5401 cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc gtccatttca
     5461 aaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt tcatcattta
     5521 aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat tgcagttcat
     5581 cactaagaat gggtatttcg cgcgcgctgc tacgggaaaa ggacaaaaag cccagcgccc
     5641 ggtttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg cgcgcggctt
     5701 cccataacgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg ccctgactaa
     5761 agttttcagg gttcagcacc ggatcaattg cgagaaagtt ttttgcctga taataactaa
     5821 cccacgcctc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt
     5881 ggcgaacaca taacgaatag taatcgtact ctagctgctg agcctgaagc tctatttcat
     5941 ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc
     6001 tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag tatcatttat
     6061 aatgataatt caaactatca cataaataag atttatatat aatttatatt attcaggcaa
     6121 tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt
     6181 taagctgttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg
     6241 attttttctt attattgcag cagaaacttc tcgaggaact gacgggtgcg aggctgctgg
     6301 ggatcggcaa ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaaa
     6361 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg
     6421 cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc
     6481 agcgccgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgtgca
     6541 atcgcaacac gttgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
     6601 gccagcccaa ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg
     6661 cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa
     6721 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
     6781 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatagt tcccgcttcc
     6841 ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg
     6901 ataattgcca ccacttcgcc aggctttacc tcaaggtcga taccgtgcag caccgtctga
     6961 ccgtgaaatt ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct
     7021 ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc
     7081 agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca
     7141 actgcgcctg acggaacagc tccggcacct gaattgtcgc ggccagcgac gtatctttta
     7201 ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
     7261 tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac
     7321 ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc agcataggcg gcagtgttga
     7381 gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc
     7441 cgtaatagat cataaacagt tgtgcgatga gcggtgtacc acgaaaaata gagatataaa
     7501 aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
     7561 cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt
     7621 tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg
     7681 gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag
     7741 aatcggccct tcatctttga aacgtttctg actgataccg tcaatcagac ccgccatcgc
     7801 ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc
     7861 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa
     7921 aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
     7981 tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
     8041 catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac
     8101 caccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
     8161 cacataaccc agcgcgccca gagcattaga accgccaacc ggaatgacat acgggcgaaa
     8221 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
     8281 gtcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa
     8341 atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt gcagaccgag
     8401 tttggcagcg actgcggcag tctggcgcac atggttagac tggatcgccc cggcagtaat
     8461 cagcgtatcg gcaccttcgc gcagagcatc tgccgcgaga aattctagct tacgtaattt
     8521 attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag
     8581 ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa actccaaccg
     8641 tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt
     8701 tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt
     8761 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagcgcttgc
     8821 agagtgccgt ctttttgcat ttccgcaatt gcatcattca ccgctttcag caggtcttca
     8881 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
     8941 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacggtcaac gaggatcgca
     9001 tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
     9061 tcgacaccct gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc
     9121 acttttttgc ctttcagatc agcagctgtt ttaatggtgc cttcgttacc ttttttcacc
     9181 agcgcctgaa tgccggaaat ggtgtaaggg gtagagaaat cgtatttttt cttgcgctcg
     9241 tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagaatccag cgacgccagc
     9301 ataccgtccc atttggtcgg ttttagggac gcctcaacgc caagatgttt tgccagctgt
     9361 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc
     9421 ggataagttc cttccagacc taccagcagc gtgccgcgct ctttgacttt attaagcaga
     9481 ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc
     9541 atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct
     9601 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
     9661 atttaatata tatcagaaga aggcaggctg gaggatttct tcacaagtcc ggtacaggtt
     9721 tggcgctgat agtgttgata cttccgtaac gcgatgcggt aattgttggt gctggtggtg
     9781 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgttc gagggaaata
     9841 ttttgctcgt gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta
     9901 cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
     9961 tcactctgcg ttttgcagtg taaatcaccg caaaatcggc acaatgccgc ccatgattgt
    10021 tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta
    10081 ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg gcaatgcgca
    10141 caatgggtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg
    10201 tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt
    10261 tataacttac ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc
    10321 cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa
    10381 tagagggtta ataccagttt ttcgcgctcc ggcagcgttt cgatagcttc catcacccgc
    10441 tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta
    10501 accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg
    10561 ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct
    10621 acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc
    10681 acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc
    10741 agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgtcgttcc ttgtagggcg
    10801 tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt
    10861 tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca
    10921 taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg
    10981 ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta
    11041 atcggacgat tagtgggtga aatgaggggt tatttggagg ttaccggtaa attccatgca
    11101 gaaaaaaccc cgccggtggc ggggaagcac gttgctgaca aattgcactt tatgttgccg
    11161 gatgcggcgt aaacgcctta tccggcctac aaaaatgtgc aaattcaata aattgcaatt
    11221 ccccttgtag gcctgataag cgcagcgcat caggcaattt ggcgttgccg tcagtctcag
    11281 ttaatcaggt tacgacgatt aaccctgcag cagagacaga acctgctgtg gtacctggtt
    11341 agcttttgcc agcacggagt taccggcctg ctggataatc tgcgctttcg acatgttgga
    11401 cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcagaca ggttggtggt
    11461 ggtgttgttc aggttggtga ccgcggaatc cagacggttt tgcaccgcac cgagggagga
    11521 gcggaatttg tcgatggagc tgatggcttc gtccagagct ttcaggggat cggcggtggt
    11581 ttcggctttg gttgctgcat cggtggtcag gctaccatct tctgtcgcat aaatagtgga
    11641 gcctgtatcg ttagtcacgc taccgttagt atgagcgaaa taattgacaa ctttactggt
    11701 ttggtctgtg attgcgccgc tgtcatcaat gtttgctgct ttgccattga tagtgacagc
    11761 attgctggtc acgttgtaag tatcactacc atcacttaag gtgaaatctt tatcctgaag
    11821 gatgctcgcc atttgcgaag ctgagataga gatatcggca acatctgccg tggtattatc
    11881 aactgccgtt gcgcccagcg ctcccaccgc ggcattaccc gcagttgcag cggctcccgc
    11941 ggctcccgca tcacccggag taccagtagt aattgaggtc gcatatgcgg cattataagc
    12001 cgcgtctttt aacacctgag cattaccacc agcagaagtg atggtcattc cttttgcttc
    12061 gattactgtc ccaacagctg ctgccgcaga gttaccatag gcatctttgc ctgtagtatc
    12121 tgtattggcc atcaggttat cccaggttgc agctttggtt gtgccactac ctgtttgggt
    12181 caggttgccc ttctcatcta aatacagcgc cttaccatcg ctggatgtaa tcgtaccatc
    12241 tttgctaacg ttaacttctt gcgatttaga accaatagtc actttagcgg taaatgtatc
    12301 gccagcgttt ggtgccagtg tgctcaacag agaatcttta gatgcagccg ttgcgctaaa
    12361 gctgtatgag ttagagtcct tatgataagt ataagtcccc ccagttgcag ctatacctgt
    12421 gccgttatta ccagcgaacg taatttcgtc gccatcttta gcagcagcca gtacattgct
    12481 tgcagtcgcg gcggcaaact ggacattttt gctataagtt acggtgccat cagcagcttt
    12541 agtacccgct gtaaaaccag ccaatgtcag attatctgta gtcgcagcgg tattcgctac
    12601 agaaccttta ccgttaacgt taaaaccagc cagattcagc gtatcagagt caattttctt
    12661 cagatcaata gtaatcgttt cgccatcgtt cgcgccgacc tgaattttca tcgagccatc
    12721 tttagacagc acgttcacgc cgttgaactg ggtctgacca gatacgcggt caatttcgtc
    12781 aagacgggat ttgatttcgt cctggatgga gtccaggtca gaggtggagt tagttccggt
    12841 cgtcgcctga accgtcagtt cacgaatacg ctgtaagttg ttgttaattt cggacagcgc
    12901 gccttcagtg gtctgcgcaa cagaaatacc gtcgttggcg ttacgtgcag cctgagtcag
    12961 gcctttaatg ttagaagtaa aacggttagc aatcgcctga cctgcggcgt catccttcgc
    13021 gctgttaata cgcaagccag aagacagacg ctcgatagaa ctcgacagcg cagactggtt
    13081 cttgttgata ttattttgag tgatcagcga gaggctgttg gtattaatga cttgtgccat
    13141 gattcgttat cctatattgc aagtcgttga ttacgtattg ggtttccacc cgtcggctca
    13201 atcgccgtca accctgttat cgtctgtcgt aaaacaacct ttagaatttt tttcacaaac
    13261 agccattttt tgttagtcac cgaaatactc ttttctctgc cccttattcc cgctattaaa
    13321 aaaaacaatt aaacgtaaac tttgcgcaat tcaggccgat aaccccggta ttcgttttac
    13381 gtgtcgaaag ataaaaggaa atcgcatggc aagtatttca tcgctgggag tcgggtcagg
    13441 tctggattta agttcaatcc ttgatagcct caccgccgcg caaaaagcga cgctaacccc
    13501 tatttcaaat cagcaatcgt cgtttaccgc taaacttagc gcctacggta cgctgaaaag
    13561 cgcgctgacg actttccaga ccgccaatac tgcattgtct aaagccgatc ttttttccgc
    13621 caccagcact accagcagca ccaccgcgtt cagtgccacc accgcgggta acgccatcgc
    13681 cgggaaatac accatcagtg tcacccatct ggcgcaggcg caaaccctga ccacgcgcac
    13741 caccagagac gatacgaaaa cggcgatcgc caccagcgac agtaaactca ctattcaaca
    13801 aggcggcgac aaagatccga ttaccattga tatcagcgcg gctaactcat cgttaagcgg
    13861 gatccgtgat gccatcaaca acgcaaaagc aggcgtaagc gcaagcatca ttaacgtggg
    13921 taatggtgaa tatcgtctgt cagtcacatc aaatgacacc ggccttgata atgcgatgac
    13981 actctcggtc agcggtgatg atgcgctaca aagttttatg ggctatgacg ccagtgccag
    14041 cagcaacggt atggaggtct cggttgcagc ccagaatgcg cagctgacgg tcaacaacgt
    14101 cgccatcgag aacagcagca acaccatcag cgacgcgctg gaaaacatca ccctgaacct
    14161 gaacgatgtc accacgggca accagacgct aaccatcact caggacacct ccaaagcgca
    14221 aacggcgatt aaagactggg tgaatgccta caactcgcta atagatacct tcagcagcct
    14281 gaccaaatac accgccgtag atgcgggagc tgatagccag agttctagca atggcgcact
    14341 gctcggcgac tccacgttgc ggacgattca gacgcagttg aaatcgatgc tgagtaatac
    14401 cgtcagttct tccaactata aaacgctggc acagattggt atcacgaccg atcccagcga
    14461 tggcaaactg gaactggatg ccgacaaact caccgctgca ctgaaaaaag atgccagcgg
    14521 cgtaggtgca ttgattgttg gcgatggtaa aaaaaccggc atcacaacca ccatcggcag
    14581 caacctgacc agttggcttt cgacaacggg cattattaaa gccgctaccg atggcgttag
    14641 taaaaccctg aataagttaa ctaaagacta caacgccgcc agcgatcgca ttgatgcgca
    14701 gatcgcgcgc tacaaagaac aatttaccca actggacgtt ttaatgacct cgttaaacag
    14761 caccagcagc tacttaacgc agcagttcga aaacaacagt aattccaagt aatcattatt
    14821 catcgggaga caggtcatgt acgcggcaaa aggcacccag gcctatgcac aaattggcgt
    14881 cgaaagcgcc gtaatgagcg ccagccagca gcagctggtc accatgctat ttgatggagt
    14941 gctgagcgca ctggttagag cgcgcctgtt tatgcaggac aacaatcagc aaggcaaagg
    15001 cgtctctttg tcaaaagcga tcaacatcat tgagaacgga ctgcgggtga gtcttgatga
    15061 agagagcaaa gacgaactaa cccaaaactt gattgctctt tatagctata tggtcaggcg
    15121 cttgctgcaa gccaatttac gcaacgatgt ctccgcagtc gaagaagtgg aagcattaat
    15181 gcgcaatatt gccgatgcct ggaaagagtc gttactctcc ccttctttga ttcaggaccc
    15241 agtctgatga accacgcacc gcatttgtat ttcgcctggc aacaactcgt cgaaaaaagc
    15301 cagctcatgt tacgcctggc aacagaagaa caatgggacg aactcatcgc cagcgagatg
    15361 gcgtatgtga atgcggtgca ggagattgca catctgactg aagagattga cccgtccacc
    15421 acgatgcagg agcagctccg cccgatgctg cgcctgattc tcgacaacga aagcaaggta
    15481 aagcagttat tacagattcg gatggatgaa ctggcgaaac tggtcggtca gtcatcggtg
    15541 caaaaatcgg tgttaagtgc ctatggcgat cagggcggct ttgtgctggc tccgcaggat
    15601 aacctctttt gaatctgaac gagtcgatgg ctcgcgaata atccgattac ggctacgctt
    15661 ctaatgttcc ccttgaatgg agtcgaagaa tgcgtaatcc cacgctgtta caatgttttc
    15721 actggtatta cccggaaggc ggtaagctct ggcctgaact ggccgagcgc gccgacggtt
    15781 ttaatgatat tggtatcaat atggtctggt tgccgcccgc ctataaaggc gcatcgggcg
    15841 ggtattcggt cggctacgac tcctatgatt tatttgattt aggcgagttt gatcagaaag
    15901 gcagcatccc tactaaatat ggcgataaag tacaattgct ggccgccatt gacgccctga
    15961 aacgtaatga cattgcggtg ctgttggacg tggtagtcaa ccacaaaatg ggcgcggatg
    16021 aaaaagaagc tattcgcgtg cagcgtgtaa atgctgacga ccgtacgcaa attgatgaag
    16081 aaatcattga gtgcgaaggc tggacgcgtt acaccttccc cgcccgtgcc gggcaatact
    16141 cacagtttat ctgggatttc aaatgtttta gcggtatcga ccatatcgaa aaccctgatg
    16201 aagatggcat atttaaaatt gttaacgact acaccggcga aggctggaac gatcaggttg
    16261 atgatgaatt aggtaatttc gattatctga tgggcgagaa tatcgatttt cgcaatcatg
    16321 ccgtgacgga agagattaaa tactgggcgc gctgggtgat ggaacaaacg caatgcgacg
    16381 gttttcgtct tgatgcggtc aaacatatac cggcatggtt ttataaagag tggatcgaac
    16441 acgtacaaga agttgcacca aagccgctgt ttattgtggc ggagtactgg tcgcatgaag
    16501 tcgataaact gcaaacgtat attgatcagg tggaaggcaa aaccatgctg ttcgatgcgc
    16561 cgctgcagat gaaattccat gaagcatcgc gcatggggcg cgactacgac atgacgcaga
    16621 ttttcacggg tacattagtg gaagccgatc ctttccacgc cgtgacgctc gttgccaatc
    16681 acgacaccca accgttgcaa gccctcgaag cgccggtcga accgtggttt aaaccgctgg
    16741 cgtatgcctt aattttgttg cgggaaaatg gcgttccttc tgtattctat ccggacctct
    16801 acggtgcgca ttacgaagat gtcggcggtg acgggcaaac ctatccgata gatatgccaa
    16861 taatcgaaca gcttgatgag ttaattctcg cccgtcagcg tttcgcccac ggtgtacaga
    16921 cattattttt cgaccatccg aactgcattg cctttagccg cagtggcact gacgaatatc
    16981 ccggctgcgt ggtggtcatg tcgaacgggg atgatggcga aaaaaccatt catctgggag
    17041 agaattacgg caataaaacc tggcgtgatt ttctggggaa ccggcaagag agcgtagtaa
    17101 ccgacgaaaa cggtgaagcg accttctttt gcaacggcgg cagcgtcagc gtgtgggtta
    17161 tcgaagaggt gatttaaatt catccccggc ggcaagccgg ggagatttca ttacggcagt
    17221 ggcgtcggta acggcgcttt atccagcgcg gcggcacact ctgccgtcgg gcgatccaca
    17281 cgctccatcg tcataccatc gtactcaatg gtgttaccgt cacgctcaac ctcgtacagc
    17341 tcacgtttta cggtaacgtt agtgagatca tcagacatca atgtcaactt acccggcacg
    17401 gcaatcaccc gctgccactg acggcaatcg agggtatcgc cctctttagt gacaatgagg
    17461 ctgcctatcg cttccgggct gaccagcgca cgttgcggcc ctttggtctg ccagtagccc
    17521 gccagccagt ccggcgcggg ggttttcacg acattgttat agttttcgac ttcggcacag
    17581 cccgccagca acatgagcgc gcctgcaatt gcgagttttt tcatcattcg tcctgcatga
    17641 gaagaaaaga ggattgtggc attaaagccc tgatgtcgcc agcctttcaa agttcagaga
    17701 gaaatttgat ctaactatta tttttgtgaa ttattacccc gaaaataaat ttgcttgatg
    17761 tatgattcgc gactcttgtt tcaaaccttc tgagttcaga ggctacattc atgtcatggc
    17821 agcaattcaa acacgcctgg ttgattaaat tctgggcgcc catccccgcg gtcatcgcgg
    17881 cgggtattct ctccacttac tattttggca ttactggcac cttttgggct gtcacgggtg
    17941 aatttacccg ttggggcggc cagctcctgc agctgtttgg cgtccatgct gaagagtggg
    18001 gttattttaa aattatccat ctggaaggat cgccattaac ccgcatcgac gggatgatga
    18061 tcctcggcat gtttggcggc tgctttgccg cagcgctgtg ggccaacaat gtcaaactac
    18121 gcatgccgcg cagccgtatc cgcattatgc aggccattat tggcggtatt atcgccggtt
    18181 ttggcgcgcg tctggcaatg ggctgtaacc tggcggcgtt ctttaccggc attcctcagt
    18241 tctcgctgca tgcctggttc tttgccatcg ccactgccat tggttcatgg tttggcgcgc
    18301 gctttaccct gctgcccatc ttccgtattc ccgtgaaaat gcagaaagtt tctgccgcct
    18361 caccgctgac gcaaaaaccg gatcaggcgc ggcgtcgttt tcgtctcggg atgctggtct
    18421 ttttcggcat gctgggctgg gcgctgctca cggcgatgaa ccaacccaaa ctggggctgg
    18481 caatgctgtt tggcgtcggc tttggtttac tgattgaacg tgcgcaaatc tgctttactt
    18541 cggcgttccg cgatatgtgg atcaccggac gtacccacat ggcgaaagca atcattattg
    18601 gtatggcggt gagtgccatc gggatcttca gttacgtaca gttaggtgtt gaacccaaaa
    18661 tcatgtgggc gggaccaaac gcggtaattg gtggtttact gtttggtttt ggcatcgtgc
    18721 tggcaggcgg ctgcgaaacc ggttggatgt accgcgcggt agaaggtcag gtgcactact
    18781 ggtgggtcgg tctgggcaat gtgatcggct caaccattct ggcgtattac tgggatgatt
    18841 tcgctccggc gctggccacc gactgggaca aaatcaacct gctgaaaacc tttggcccga
    18901 tgggtggcct gctggtgaca tatttgctgt tgtttactgc gctgatgttg attatcggct
    18961 gggaaaaacg cttcttccgc cgtgcggcac cgcagactgc taaggagatc gcatgaaaaa
    19021 tatcattcct gattaccgtc ttgatatggt gggtgaaccc tgcccttatc cggcagtcgc
    19081 aacccttgag gcgatgccgc agttaaaaaa aggtgagatc ctggaagtgg tgagcgactg
    19141 tccgcagtcg atcaataata ttccactgga tgctcgtaat cacggctata cggtgctgga
    19201 tattcagcaa gacgggccga ccattcgtta tttaattcag aagtaatccc tcattcccgc
    19261 tgtggccaac agcgggaata tcgttgcctc tgtaccacat agattttacc cgccccctct
    19321 cctattcttt tcagtgtgct ctcaggaggt tgctatgtgt ggacgctttg cccaatccca
    19381 aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctatga
    19441 tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga
    19501 acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga
    19561 taaaccgccg ctgattaacg cccgcgtaga aactgcggcc accagtcgta tgtttaaacc
    19621 gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga
    19681 aggcgacaaa aaacagcctt attttatcta tcgtgctgac ggacaacctg tttttatggc
    19741 cgcgataggt agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt taattgtcac
    19801 tgcagcagca gaccagggtc tggtagatat tcatgaccgc cgcccgctgg tactgtcgcc
    19861 agaagctgcg cgggaatgga tgcggcaaga gattggcggt aaagaagcat cagaaattgc
    19921 taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc gcgcggtagg
    19981 taatgtcaaa aaccagggcg cggagttaat tcaacctgtt tgataagcca ggagactatt
    20041 gatcaataat actgcgtcat aagaaatctc tattagacaa agtagacgct ccttccacat
    20101 gcgccacaag aaacctctat tccagtgaca caattacgcc tgattatttc cctagcccgg
    20161 aatatgaata aaaacatcaa caaagattaa attattcaaa cgttactgtt gcgtctgcgg
    20221 tcgtaacaac atccgacgtg gtgatgtgta tcaaatgagg cctctgatga tcagagggtt
    20281 tatcgcgtat acggatcgtg gatggcagaa aactttcaga atcaggtttc cattttaaac
    20341 cagaaattga gtgagtttgc cccatccatg ccccacgcgg tgagatcgga tgtaattaac
    20401 aacaggttac aaaacctaca cctgcatgct catcacttcc tgatacgccg ccaccagctt
    20461 attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat cggtcatcac
    20521 atcgtttaac gccacgccgg gttcaccgag agtgaatttt tctgcctgcg tgcgggcagc
    20581 tgtttgtgta tcactaatgc gatcgagcgc ggcgtgcagc tgcccggcaa aactaatggt
    20641 cggttgcggc agtgattcct gcgcacgcgc actcatcgcc gtagcctgta actggctgat
    20701 aaccccttca atcccctgta tcgctgacat tctcgtctcc cggataattt ctggtagcaa
    20761 agcctaccag taagtcaata agacaaaggc gctaaataac aacaaaaaaa cgggtttatt
    20821 ggcggataga aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtttgctga
    20881 cccgggagtg agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaggtg
    20941 cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc
    21001 gaatccgaaa attccattga ttgttgccgg ttccgcggca gtggcggtca tggtcgcact
    21061 gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga
    21121 tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc
    21181 cagcggcgct attgaagttc cggcagataa agttcacgaa ctgcgtctgc gcctggcaca
    21241 acaaggtttg cccaaaggcg gcgcggtcgg tttcgaacta ctcgatcagg aaaagtttgg
    21301 tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg agctttctcg
    21361 taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc
    21421 gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc
    21481 cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt ccagcgcggt
    21541 tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc tgttaaccca
    21601 gtccaatacc agcgggcgcg atcttaatga tgctcagttg aaatatgcca gcgatgtcga
    21661 aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta acggtaatat
    21721 tcacgcccag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg aagaacagta
    21781 tcgccctaac ggtgatgaat ctcaggcggc gcttcgttca cgccagctta atgagagcga
    21841 gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc
    21901 tgcgaataac gcgccaatca gcacgcctcc ggcaaatcaa aataaccgcc agcagcaggc
    21961 gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca gtaactacga
    22021 agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt
    22081 cgcggttgtg gtgaattaca aaaccttgcc agatggcaaa ccgttgcctc tcagcaacga
    22141 acagatgaag caaattgaag atctgacccg cgaggcgatg ggcttttctg aaaaacgcgg
    22201 tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg gcggagaact
    22261 gccgttctgg caacagcaag cgtttatcga tcagttgctg gctgccggtc gctggttgct
    22321 ggtactgctg gtggcgtggc tgctgtggcg gaaagcggta cgtccgcagc taacacgtcg
    22381 cgccgaggcg gtgaaggcta tgcagcaaca ggcacaggcc cgcgaggaag tggaagatgc
    22441 ggtggaagtc cgcctgagca aagacgaaca actacaacaa cggcgcgcta accaacgact
    22501 gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc cgcgcgtggt
    22561 ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca ggcaccgata
    22621 aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg ttcaagcacc
    22681 tctcccagcg tgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg cagatctcca
    22741 acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag tttgccgcac
    22801 tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg ggtgaagaac
    22861 gtgccgccag cctgctggaa gatattctcg aaactcgcga taccgccagc ggtattgaaa
    22921 cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag catccgcaaa
    22981 ttatcgccac cattctggtg catctgaagc gcgcgcaggc cgccgatatt ctggcgttgt
    23041 tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc ggcgtgcagc
    23101 cagccgcgct ggcggaactg accgaagtac tgaatggctt gctcgacggt cagaatctca
    23161 agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg atgaaaactc
    23221 agcaggaaga agccgttatt accgccgtgc gcgaattcga cggcgagctg gcgcagaaaa
    23281 tcatcgacga gatgttcctg ttcgagaatc tggtggatgt cgacgatcgc agcattcagc
    23341 gtctgttgca ggaagtagat tccgaatcgc tgttgatcgc gctgaaaggg gccgagcagc
    23401 cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt ctgcgcgacg
    23461 atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtgga aaacgaacag aaagcgattc
    23521 tgctgattgt gcgccgcctt gccgaaactg gcgagatggt aattggcagc ggcgaggata
    23581 cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc gccaccacag
    23641 gcagagtttg tgcccatggt cgagccggaa gaaaccatca ttgaagaggc cgaacccagc
    23701 cttgagcagc aactggcgca actgcaaatg caggcccatg agcaaggtta tcaggcgggg
    23761 attgccgaag gtcgccagca aggtcatgag cagggctatc aggaaggact ggcccagggg
    23821 ctggagcaag gtctggcaga ggcgaagtct caacaagcgc caattcatgc ccggatgcag
    23881 caactgatca gcgaatttca aactaccctt gatgcacttg atagtgtgat tgcttcgcgc
    23941 ctgatgcaga tggcgctgga ggcggcacgt caggtcatcg gtcagacgcc aacagtggat
    24001 aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt attcagcggt
    24061 aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat gctcggcgca
    24121 accttaagtt tgcatggctg gcgcttgcgg ggcgatccca cactccatcc tggcggctgt
    24181 aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg gcaagaactc
    24241 tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc tggctaacca
    24301 cgctggataa cttcgaagcc aaaatggcgc agttgcctgc ggtacgtcgc tacgggcgat
    24361 taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg ctcggcgcaa
    24421 cctgtgtcat tgagcgtcag aacggcagcg aaacgcacga agtagaaagc gaagtcgttg
    24481 gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt gtcctgcccg
    24541 gcgcgcgggt ttatgccaaa aacatttcgg ctgaaggact gcaaagcggc aagcagttgc
    24601 cgctcggtcc ggcgttatta ggtcgcgttc tggacggcag cggtaaaccg ctcgatggcc
    24661 tcccctcccc cgatacgacg gaaaccggtg cgctgattac cccaccattt aacccattgc
    24721 aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat gccctgctta
    24781 ccgtcgggcg cgggcagcgt atgggactgt ttgccgggtc cggcgttggt aaaagtgtgc
    24841 tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt ttgattggtg
    24901 aacgcggacg cgaagtaaaa gattttattg agaatatcct cggtgccgaa gggcgtgcac
    24961 gctccgtggt gattgccgct ccggcggatg tctctccgct tctgcgaatg caaggtgccg
    25021 cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg ttgctgatta
    25081 tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg attggcgaac
    25141 cacccgccac caaaggttat cccccgtcgg tgtttgccaa attaccggca ctggtcgagc
    25201 gtgccggaaa tggcattagc ggcggcggat cgattaccgc gttttatacc gtgctcaccg
    25261 agggcgatga ccagcaggac cccattgccg actccgcgcg ggcaatcctc gacggtcaca
    25321 ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat attgaagcgt
    25381 cgatcagccg cgcaatgacg gcgttgatca gcgagcaaca ttacgcgcga gtgcgcacct
    25441 tcaaacagct gttgtcgagt tttcagcgta accgagatct ggttagcgtc ggcgcgtatg
    25501 ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag ctggagggct
    25561 atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag gggctggagc
    25621 gtattttccc gacagtgtca taacccagga gataacggca gatggcagaa catggcgcgc
    25681 tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgcgcgc ctgctgggtg
    25741 aaatgcgtcg cgggtgtcag caggcggaag aacagctcaa aatgctgatc gattatcaga
    25801 atgaatatcg caataacctc aacagcgata tgagtgccgg gatgaccagc aaccgctgga
    25861 tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat cgccagcaac
    25921 ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa aagaaacaac
    25981 gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa
    26041 accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg aggaaacctg
    26101 aatgattcgc ttagcgccct taattaccgc cgacgttgac accaccacat tgcctggcgg
    26161 caaagccagc gatgctgcac aagattttct cgcgttgttg agcgaagcat tagcaggcga
    26221 gacaactacc gacaaagcgg ccccccagtt gctggtggca acagataagc ccacgacaaa
    26281 aggcgagccg ctggtcagcg agattcttgc cgatgcgcaa caagcggatt tactgatccc
    26341 tgtggatgaa acaccgcctg tcatcaacga cgaacaatcc acatcaacac cattaaccac
    26401 cgctcaaacg atgacgttgg ctgcggtggc tggcaacaat acggcaaaag acgaaaaagc
    26461 ggatgatctg aatgaagacg tcaccgcaag cctgagcgcc ctttttgcga tgttgccgga
    26521 ttttgacaat acgcccaaag tgactgatgt gccgtcaacc gtgttaccgg cagagaaacc
    26581 aacgctcttc acaaaactga cttctgcgca actcacaaca gcacagcctg atgatgcccc
    26641 cggcacgcca gctcagccat taacaccgct ggtagcagaa gcccagagta aagcggaagt
    26701 catcagcaca ccttcaccgg tgaccgctgc cgccagcccg ctaatcactc cacaccagac
    26761 acagccactg cccaccgtcg ccgcgcctgt gttgagtgca ccgctgggtt ctcacgaatg
    26821 gcaacaatca ttaagccagc atatttcgct gttcacccgc caggggcaac aaagtgcaga
    26881 gttgcgtctg cacccgcagg atttaggtga agtgcaaatc tccctcaaag tggatgataa
    26941 ccaggcgcaa atccagatgg tttcaccgca tcaacacgta cgcgccgccc tggaagcagc
    27001 gcttccggta ctgcgaacgc agctggccga aagtggcatt cagttagggc aaagcaacat
    27061 cagtggcgaa agctttagtg gtcagcagca ggccgcttcc caacaacagc aaagccaacg
    27121 cacagtaaac catgaacctc tggcggggga agacgacgat acgcttccgg ttcccgcctc
    27181 tttacaaggg cgcgtaacag gcaacagcgg cgttgatatt ttcgcctaac gtcagaggta
    27241 gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga taattagccg
    27301 ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat aagcaagaaa
    27361 agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc ctgtgccagc
    27421 gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc
    27481 gtcgtgccct caccggtctt ctacgcgctg gataccttta cggtcaatct gggcgatgcg
    27541 gatcgcgtgc tttatatcgg cataaccctg cgcctgaaag atgaagctac ccgctcgcgg
    27601 ctaagtgagt atttgccgga agtccgtagc cgtttgctgt tactgttttc gcgtcaggat
    27661 gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat taaaaccaca
    27721 ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc
    27781 gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg aaattgatgc
    27841 gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta gcggcgaaag
    27901 tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc
    27961 gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctat tcaacctgct
    28021 gcgtcgtagc ccggatataa ccgtcggggc catccgcatt cagccgtacc atgaatttgc
    28081 ccgcaacctg ccggtgccga ccaacctgaa ccttatccat ctgaaaccgc tgcgcggcac
    28141 agggctggtg gtgttttcac cgagtctggt gtttatcgcc gtggataacc tgtttggcgg
    28201 cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccataccg aacagcgcgt
    28261 catcaaccgc atgttaaagc tggcgcttga aggctatagc gacgcctgga aggcgattaa
    28321 tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg aaatttacca atatcaccac
    28381 ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca acctgaccgg
    28441 cgaatttaat atctgtctgc cattcagcat gatcgagccg ctacgggaat tgttggttaa
    28501 cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca
    28561 ggtgcagcat tcacagctgg agttggtcgc caactttgcc gatatctcgc tacgcctgtc
    28621 gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg accgcatcat
    28681 cgcccatgtt gacggcgtcc cggtgctgac cagtcagtac ggcactctca acggtcagta
    28741 tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg aggaacagcc
    28801 caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga cgatctgtgg
    28861 gctgaagcgt tgagcgaaca aaaatcaacc agcagcaaaa gcgctgccga cgcggtgttc
    28921 cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct gattatggat
    28981 attccggtca agctgaccgt cgaactgggc cgtacgcgga tgaccatcaa agagctgttg
    29041 cgtctgacgc aagggtccgt cgtggcgctg gacggtctgg cgggcgaacc gctggatatt
    29101 ctgatcaacg gttatttaat tgctcagggc gaagttgtcg tagtcgctga taaatatggc
    29161 gtgcggatca ccgatatcat tactccgtct gagcggatgc gccgcctgag ccgttagtga
    29221 tgaataacca cgctactgtg caatcttctg cgccggtttc tgctgcgcca ctgctgcagg
    29281 tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg ctggtaaaac
    29341 ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc gccagtgctt
    29401 cactgggcgc gcgtgaacgg gttgtggtgg tcgatgtgga agatgcacgg ctggtgctcg
    29461 gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca ccaacggaag
    29521 agataccgca gaccgatttt cagtcggtta tgaaaaactt gcttaagcgt agcgggagat
    29581 cctgatgcgt cgtttgttgt ctgtcgcacc tgtccttctc tggctggtta cgcccctcgc
    29641 cttcgcgcaa ctgccgggta tcaccagcca gccgctgcct ggcggtggac aaagctggtc
    29701 gctcccggtg caaacgctgg tgttcatcac ctcgttgacg tttattccgg caattttact
    29761 gatgatgacc agtttcaccc gcatcatcat tgtttttggt ttattgcgta acgcgttggg
    29821 aacaccctcc gcgccaccta accaggtatt gctgggactg gcgctgtttt tgaccttttt
    29881 tattatgtcg ccggtgatcg ataaaattta tgtcgatgcg taccagccat tcagcgaaga
    29941 gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct
    30001 gcgtcagacc cgcgaggcgg atttagggtt gtttgccaga ctggcgaata ctggcccgtt
    30061 gcagggacct gaagctgtgc cgatccgcat tttgctcccg gcctacgtga ccagcgagtt
    30121 gaaaaccgca tttcagatag gcttcacgat tttcattcct tttttgatta tcgacctggt
    30181 gattgccagc gtgttgatgg cattggggat gatgatggtt cccccagcca caattgctct
    30241 gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct
    30301 ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg
    30361 gactgaagcg atgaaagtcg cgctggcact ggctgcccca ctgttgctgg tggcgctggt
    30421 cacgggtctt atcatcagta ttttgcaggc cgccacccag attaacgaaa tgacgctgtc
    30481 gtttattccg aaaatcatcg ccgtatttat cgccattatt attgccggac cgtggatgct
    30541 caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata tcatcgggta
    30601 ggccgtacaa tgatgcaggt aacaagcgat caatggcttt cctggttaag cctgtacttc
    30661 tggccgttac tgcgcgtgct ggcgctcatt tctaccgcac caattctgag cgaacgcagc
    30721 gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgctat tgccccatca
    30781 ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc cgtgcagcag
    30841 atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc tgtgcgaacc
    30901 gctggtgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacgtttgt cgatccggcc
    30961 agccatctca atatgcccgt tttagcgcgt atcatggata tgctggcgtt actgctgttc
    31021 ctgacattta acggtcattt atggttgatt tcactgctgg ccgatacctt tcacaccctg
    31081 ccaattggtg gcgaaccgtt gaacagcaat gcgtttctgg cactcaccaa agcagggagt
    31141 ttgattttcc ttaacgggct gatgctggca ttaccgctca ttactctgct gctgaccctg
    31201 aatctggcat taggcttact taatcgtatg gccccgcaat tatccatttt tgttattgga
    31261 tttccattaa ctctgactgt cggtatctct ttaatggcgg cattaatgcc gttaattgcg
    31321 cctttttgcg aacatttatt cagtgagatg tttaatttgc tggctgatat tattagtgaa
    31381 ttgccattaa tataattccg taacgtttat catgttatcc taaggattat ccgaaaaata
    31441 atacctacga acatcttcca ggatactcct gcggcgaaat atttgtttta agctcactca
    31501 catatcgcaa catttacttt actttaagac aattccaggc aaattataca acactttacg
    31561 ggatagtaag tccgcctgaa aaatcgcgag agtggcgcat taggtgaccc atgttgttcc
    31621 gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg agtgagggta
    31681 tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt taaccgggta
    31741 tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg ttgatgacct
    31801 tgccatagct tgtgattcac agcgcccttc agtggtgttt attaatgagg actgtttcat
    31861 ccacgatgct tctaacagtc agcgtatcaa gctcatcatt aatcaacatc ccaatacgtt
    31921 atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat tggtcagaaa
    31981 aaatttattg atcagttcta aatcgattaa accggaatct ctcgacgata tccttggcga
    32041 tattctgaaa aaagagacaa cgataacctc gtttttaaat atgccgacgt tatcattgag
    32101 ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca ttcaaatctc
    32161 tgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa
    32221 gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg ataatgtgac
    32281 taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtggtttcc caccagtcag
    32341 gccgaattag attactctgc tttctccaca aagataccgt cctgatgccc tgcttcatta
    32401 aagaaccaga tgccgagcgg gtagtcttcc agcgaaacca ggtacattgt gccttcacta
    32461 aactcctcaa ctgccagtac cacgccagga cgacgcggac cgccatccgt tttgactgtt
    32521 acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt agaacaattt
    32581 cgtttttctg gcagcgccag gcgcgcgcga gtgctgattt tctcgacggt ctatacttaa
    32641 gagatgccag gcggacttaa cgactggccg caacaacaga gtaacggttg cgaggaaaga
    32701 tgatgaaaac cgcaaaagag tacagcgata ccgcaaaacg tgaggtcagc gtcgatgtcg
    32761 atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc agccagaacg
    32821 attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa ttggcggatg
    32881 ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga atgcacaata
    32941 aaaaaatccc gaccctgagg gggtcgggat gaaacttgct taagcaagaa gcacttaaaa
    33001 aattcgttac accaggaaat ctgatgtgtt catcacctta tccgcaattt ttttcgctga
    33061 caagaaaata ttcgtgatat gaatgattaa cgctacttat gaatagaaat gtgacgcgcg
    33121 tcaccccttg gtggctctgg cgtcaacggg atcgctcccg aaaaaatcat ctttgataat
    33181 ttgtgctgtt ttgagaatca tcctggtaag gggagtactg cgcaaccatg ctttcaattc
    33241 aacaaccact actggttttt agcgatcttg atggcaccct gctggacagt catagttatg
    33301 actggcaacc agctgccccc tggctcagcc gtttacgcga agcgaatgtt cccgtcattc
    33361 tctgtagcag taagacatca gccgaaatgc tgtacttgca aaaaacgttg gggctacaag
    33421 gtttaccgct gattgccgaa aatggcgcag tgatccagct tgctgagcaa tggcaggata
    33481 tagacggttt tccacgcatc atctcaggta ttagccatgg cgaaatcagc caggttttaa
    33541 atacgctacg cgagaaagaa cattttaaat tcacaacttt tgatgatgtc gacgatgcaa
    33601 ccatcgccga atggacggga ttaagccgta gccaggcggc gctgacgcag ctacatgagg
    33661 cgtcggtaac gctaatctgg cgcgacagtg acgagcgtat ggcacaattt accgctcgtc
    33721 tgaacgaact gggcttgcag tttatgcagg gtgcgcgctt ctggcacgtc ctggatgcct
    33781 ctgccggaaa agatcaggct gccaactgga ttatcgcgac ctatcaacaa tcgtcaggca
    33841 aacgcccaac cacacttggc ctgggcgatg ggccaaacga tgcgccctta ctggaggtaa
    33901 tggattacgc ggtgattgtg aaagggttaa accgtgaagg ggtgcatctg catgatgagg
    33961 atccggcccg cgtctggcga acgcagcgtg aaggaccgga aggctggcgt gaagggctgg
    34021 accatttttt ctccgcccat taagcgttat cgctcgcgaa tacccgattg cgcccggcct
    34081 gtttagcgag ataaagtcga cggtcggcca gtgactggag ttgttcaaaa tcataatcac
    34141 cggtttcctc gctgctgctt acccccagcg aggcactgat gcgtatcgtc gtactcttgg
    34201 cgatcaacat ctctttttca tttagcttca ggcgaatacg ttctgcaacc tccgcagcct
    34261 gcgtcaggct cgcgcctggc agaatcacac aaaactcctc accaccgacc cgcccggcaa
    34321 catcctgcgc tcgcaaggaa ctgctaatta atccggcagc atgagaaaga acacggtcgc
    34381 ccgcctgatg accaaagcgg tcattaatcg ctttaaaatg gtcaaggtcg acctgaatga
    34441 cagaaaaagg atgttggtgc gtctgacaca atttagcgag cggacgggct ttttcgaaca
    34501 gtgcgccacg gttatataaa cgcgttaagg tgtcgtgcca cgcctgccac tgcaacgagc
    34561 tttgcaaaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag atgagtaaca
    34621 tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg aaatcgccgc
    34681 gcacgccttc gcttagcgta tggacgcgca ccagcacacc gtcgaaatgg tccagacgtt
    34741 cccagctaac atagcgactg ttcatacgaa tgccgccccg cgtgtcatgt tccattgcct
    34801 gcgccagcaa ggccagttca cgaggatcaa aaatattccc tgttggatgg tcaggattgg
    34861 aagaggtcaa taatctcagc ttactgtcat agagctgata ctcaccatcg agttttttat
    34921 cgatggcgtt tcttaaaaat tgctgcatgg tacgcacggg aatactcatc cccagcacgc
    34981 cataccagta gttttcactg tctaccggaa cactgacggt aacctgcggt tcagtattgc
    35041 tggcgtgttc cggttgcgaa gtgaaccagc gtaccgcgcg gtgacgattt tctcgttgcg
    35101 aatggccgac aaaccagggt tgggtgacat agccgtaata acgcgttggt acattgcgcg
    35161 taaacaaggt cggctgcgtc gaaacgtaaa atccggcacg cgagacatac atcgcctgtt
    35221 caaccatcga cgaggagttg tgtgccagtc gcagcaagta accaacttcc agtgcagcgg
    35281 taatttcatt gtcgaggctt tcattttcgc gagacaggag attcccctcg ctgactaatg
    35341 catccgacac accattgact ggcagggtgc gtcgtcggtt gagttcgatt tgccaggcgt
    35401 gctcgtcgcg atgctgttcg aactcggtta cggcattacg cagagaggtg aaatccagtg
    35461 gcgctacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg acgttatact
    35521 gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctgg ctggagatat
    35581 aggcatcttc cagcaccacc acttcacgcc aggtgagcaa ggtggaaaaa agcaatacca
    35641 cgataaagca gagattaacg acatgaccag gccccaggcg gcgtgcgagt tttttcaacc
    35701 agctctggtt ttccattttt gtctcgtgct gcaccctgac gactcccaac ctgctttttt
    35761 atgattctgg cataacctgg cgatagcgta ccgcaaaaca gcgataattt tataatggtg
    35821 gcatgataag acgcgacaga catcgatagt tttataacgt aggcatgata agacgcgata
    35881 gcgtcgcatc aggcaattca gtgcagacac tggcacaccc gaccggatgc gacgtgagcg
    35941 ccacatccgg tcgggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct
    36001 cccccggaac aagctcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc
    36061 aacgacactc aaccataccg ctggagtatc cggtgacaat catccgcggg ccgccctctt
    36121 taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac
    36181 ttgttcacct tagttgaaga tggcgaattt tgcataatag cctgtgcgag tattaatgtg
    36241 cctgaccgcg tattttcgct acggctttca ctcccagcac cacgatgccg ccgatgataa
    36301 atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc
    36361 cggcgaaatg ttcaatggcg tgatgcagcg gcgcaattcc atgtaccaca atcccaccgc
    36421 cgacgaggaa catcgccagc gtgccgacaa tcgataacgc cttcatcaac cagggcgcaa
    36481 tgatcaataa ccctttacct aatgcctgca tcagcgcgct ggatttttcc gccagccaat
    36541 accccaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta
    36601 ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcttcg gccacaatcc
    36661 ccagcgtgat ggcgacgatt tccgcagaca atataaaatc ggtacgaatc gcccctttta
    36721 ttttgtcttt ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctgac
    36781 tctgcgccgg atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc
    36841 cttcaaagca gagaaacgcg ccgccaatca tcaacagtgg cgtaatcgcc cacgggataa
    36901 acgcactgat gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg
    36961 ccacgcccca gaccacggga agttcccggt tggcgcgcac gcctgaaact tgttgcgcat
    37021 tgagcgataa gtcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca
    37081 cggagatatc gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg
    37141 ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca
    37201 gctctgatac ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct
    37261 cgccagcaga atggttttca cgtttactat gaaccacctt tttatttcag ccgtgaggga
    37321 ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt
    37381 ggggctcaac ctattttgtc attcggattg gcgtggaaag ctggcctccg ttaatgatgg
    37441 cgggcgttcg attcctggca gccggtattt tattgctggc atttttgcta atgcgcggac
    37501 acaaacttcc cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg
    37561 ctgtcggtaa tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg
    37621 ccgttgtggt tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg tttggcatta
    37681 aaacgcgcaa actggaatgg gtgggtatcg ccattgggct tgccggaatc atcatgctca
    37741 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    37801 ttagctgggc gtttggctcg gtttatggct cgcgcattac cttacctgta gggatgatgg
    37861 cgggtgcgat tgagatgctg gcggctggcg tggtgttaat gattgcgtcg atgattgcgg
    37921 gtgaaaaact gacggcgctc ccttcccttt caggcttcct tgcggtcggc tatctggcgc
    37981 tgtttggttc gattatcgcc attaacgctt atatgtattt aatccgcaac gtcagtccgg
    38041 ctctcgccac cagctacgcc tacgttaacc cagtggtcgc ggtcttgctg ggtacggggc
    38101 tgggtggcga aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg
    38161 tggtactggt cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta
    38221 ttccggatgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    38281 tcgccgcaga tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc
    38341 ccgcgtaacg cacattccca gacaatcaat acgcgccagc cgagttcctg caaatggctg
    38401 atatcacgac gatcgcgctc gacattttta cctatcttct ccagccaaaa atctgttcgc
    38461 gtggcgggga ctttaaacag gtagcaatgg tgatggtgcc agaagcaacc gtgggcaaat
    38521 atcacacagc gaaattcatc aaggacaaaa tccgggcgtc cgggcagact ggcgtcctga
    38581 acgcgaaacg ccaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta
    38641 tcacgcgtgg caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc
    38701 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    38761 tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat aagcctgagt
    38821 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    38881 acattcccga ggcgttaacc gacgtgggcg atgttgctga ttcagcggat cgtcaaagtc
    38941 tttctcaccc gttgccatat cccagccgcg atcgattaaa atttccgcgc catctttgta
    39001 ataacgcgca gacagcgtac gcgtgacgct ttgcggattg ttcggataaa ccattccata
    39061 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    39121 taccggcgtc aggatatatt tcgcctcgac catcgggtcc agcaactgcg ccagcgtcac
    39181 tcgctgtgca gggaaacatt tgctgatatc acgcagagta aaatcggctt tcagattcag
    39241 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagaa aatgtttgcc
    39301 gtcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcagccacgt catagcccag
    39361 ttcgtccagc gtctgcatga tgatgcggaa cgttttaccc tggtcgtgac ttttcaggtt
    39421 tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc
    39481 aaagaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    39541 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    39601 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg
    39661 gctgagggtg atgtcgcgga tatcctcatt aaaatgatgc gtcgccgggt cgcaataatg
    39721 gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc
    39781 accaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg
    39841 aaacgcataa tgcggatgat gtgccggtgg tttgggtaac aacgtttgca gatgggcgaa
    39901 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    39961 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata
    40021 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    40081 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    40141 taattactgc cgtttagggc cacgcgctca gccagcaggt gaaaacgcat ctatcacctt
    40201 gtgatcgata ccttcattct cccccgccag ttcggcaccg agcttcgcca taaactcgac
    40261 taaaaattgc gcgttgtgct gtgccagctg cttgcctctt gcggtttgca tggtttgcgg
    40321 taatttcagt aactttgtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg
    40381 atgctgtgcg aacggatctt cgccatcaaa cagcgccaag cccaatgccc ctgaaacggc
    40441 aaatacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat
    40501 tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg
    40561 acaaacggcc tcaattttct ccgccggaaa ttgcacgaac tcttcggtaa tgctgccaac
    40621 ttactgattt agtgtatgat ggtgatttta aggtgcttgc gtggcttcca tttccatcag
    40681 atgtccttcc tgctccgcta ctgaaggcgt ggtgcgtaac ggcaaaagca ctgccggaca
    40741 tcagcgctat ctctgctctc attgccgtaa aacatggcaa ctacagttca cttacaccgc
    40801 ctctcagccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg tcggatgtcg
    40861 cgccagtgca cgcattatgg gcgttggcct caacacggtt ttacgtcact taaaaaactc
    40921 aggccgtagt cggtaacctc gcgcatacaa ccgggcagtg atgtgattgt ctgcgctgaa
    40981 atggacgaac agtggggcta cgtcggtgct aaatcacgtc agcgctggct gttttacgcg
    41041 tatgacagga tacggaggac ggttgtggcg cacgtcttcg gtgaacgcac tctggccaca
    41101 ctggagcgtc ttctgagcct gctgtcggcc tttgaggtcg tggtatgg
//
LOCUS       DAPHJE010000030        41072 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPHJE010000030 DAPHJE010000000
VERSION     DAPHJE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.030"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            315..1604
                     /gene="sdaC"
                     /locus_tag="RH222_001608"
     CDS             315..1604
                     /gene="sdaC"
                     /locus_tag="RH222_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV3796651.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1662..3029
                     /gene="sdaB"
                     /locus_tag="RH222_001609"
     CDS             1662..3029
                     /gene="sdaB"
                     /locus_tag="RH222_001609"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV3796652.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3141..3896
                     /gene="xni"
                     /locus_tag="RH222_001610"
     CDS             3141..3896
                     /gene="xni"
                     /locus_tag="RH222_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV3796653.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3951..5099)
                     /gene="fucO"
                     /locus_tag="RH222_001611"
     CDS             complement(3951..5099)
                     /gene="fucO"
                     /locus_tag="RH222_001611"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV3796654.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVISDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5127..5774)
                     /gene="fucA"
                     /locus_tag="RH222_001612"
     CDS             complement(5127..5774)
                     /gene="fucA"
                     /locus_tag="RH222_001612"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV3796655.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6321..7637
                     /gene="fucP"
                     /locus_tag="RH222_001613"
     CDS             6321..7637
                     /gene="fucP"
                     /locus_tag="RH222_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV3796656.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7670..9445
                     /gene="fucI"
                     /locus_tag="RH222_001614"
     CDS             7670..9445
                     /gene="fucI"
                     /locus_tag="RH222_001614"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV3796657.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9554..10972
                     /gene="fucK"
                     /locus_tag="RH222_001615"
     CDS             9554..10972
                     /gene="fucK"
                     /locus_tag="RH222_001615"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV3796658.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVISSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            10974..11396
                     /gene="fucU"
                     /locus_tag="RH222_001616"
     CDS             10974..11396
                     /gene="fucU"
                     /locus_tag="RH222_001616"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV3796659.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11454..12185
                     /gene="fucR"
                     /locus_tag="RH222_001617"
     CDS             11454..12185
                     /gene="fucR"
                     /locus_tag="RH222_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV3796660.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12229..13329)
                     /gene="rlmM"
                     /locus_tag="RH222_001618"
     CDS             complement(12229..13329)
                     /gene="rlmM"
                     /locus_tag="RH222_001618"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV3796661.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13322..13717)
                     /locus_tag="RH222_001619"
     CDS             complement(13322..13717)
                     /locus_tag="RH222_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV3796662.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13736..14653)
                     /gene="gcvA"
                     /locus_tag="RH222_001620"
     CDS             complement(13736..14653)
                     /gene="gcvA"
                     /locus_tag="RH222_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV3796663.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15004..15231)
                     /locus_tag="RH222_001621"
     CDS             complement(15004..15231)
                     /locus_tag="RH222_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV3796664.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15423..16628
                     /gene="csdA"
                     /locus_tag="RH222_001622"
     CDS             15423..16628
                     /gene="csdA"
                     /locus_tag="RH222_001622"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV3796665.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16628..17071
                     /gene="csdE"
                     /locus_tag="RH222_001623"
     CDS             16628..17071
                     /gene="csdE"
                     /locus_tag="RH222_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV3796666.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17122..17928)
                     /gene="tcdA"
                     /locus_tag="RH222_001624"
     CDS             complement(17122..17928)
                     /gene="tcdA"
                     /locus_tag="RH222_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV3796667.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18167..19264)
                     /gene="mltA"
                     /locus_tag="RH222_001625"
     CDS             complement(18167..19264)
                     /gene="mltA"
                     /locus_tag="RH222_001625"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV3796668.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19473..19549
                     /locus_tag="RH222_001626"
     tRNA            19473..19549
                     /locus_tag="RH222_001626"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19507..19509,aa:Met,seq:cat)
     gene            complement(19624..20877)
                     /gene="amiC"
                     /locus_tag="RH222_001627"
     CDS             complement(19624..20877)
                     /gene="amiC"
                     /locus_tag="RH222_001627"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV3796669.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21109..22440
                     /gene="argA"
                     /locus_tag="RH222_001628"
     CDS             21109..22440
                     /gene="argA"
                     /locus_tag="RH222_001628"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV3796670.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22502..24328)
                     /gene="recD"
                     /locus_tag="RH222_001629"
     CDS             complement(22502..24328)
                     /gene="recD"
                     /locus_tag="RH222_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV3796671.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24328..27870)
                     /gene="recB"
                     /locus_tag="RH222_001630"
     CDS             complement(24328..27870)
                     /gene="recB"
                     /locus_tag="RH222_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV3796672.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27863..30751)
                     /gene="ptrA"
                     /locus_tag="RH222_001631"
     CDS             complement(27863..30751)
                     /gene="ptrA"
                     /locus_tag="RH222_001631"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV3796673.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30927..34295)
                     /gene="recC"
                     /locus_tag="RH222_001632"
     CDS             complement(30927..34295)
                     /gene="recC"
                     /locus_tag="RH222_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV3796674.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKSHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34308..34631)
                     /locus_tag="RH222_001633"
     CDS             complement(34308..34631)
                     /locus_tag="RH222_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV3796675.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34616..35023)
                     /locus_tag="RH222_001634"
     CDS             complement(34616..35023)
                     /locus_tag="RH222_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV3796676.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(35020..35583)
                     /locus_tag="RH222_001635"
     CDS             complement(35020..35583)
                     /locus_tag="RH222_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV3796677.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35574..36044)
                     /locus_tag="RH222_001636"
     CDS             complement(35574..36044)
                     /locus_tag="RH222_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV3796678.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(36229..37023)
                     /gene="thyA"
                     /locus_tag="RH222_001637"
     CDS             complement(36229..37023)
                     /gene="thyA"
                     /locus_tag="RH222_001637"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV3796679.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37030..37905)
                     /gene="lgt"
                     /locus_tag="RH222_001638"
     CDS             complement(37030..37905)
                     /gene="lgt"
                     /locus_tag="RH222_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV3796680.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38056..40302)
                     /gene="ptsP"
                     /locus_tag="RH222_001639"
     CDS             complement(38056..40302)
                     /gene="ptsP"
                     /locus_tag="RH222_001639"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3796681.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40315..40845)
                     /gene="rppH"
                     /locus_tag="RH222_001640"
     CDS             complement(40315..40845)
                     /gene="rppH"
                     /locus_tag="RH222_001640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV3796682.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
BASE COUNT         9856 a        11172 c        10222 g         9822 t
ORIGIN      
        1 tcaacctaca tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt
       61 gcccttaaat tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc
      121 caagtagatt attgtatttg ggatcaagat cactgataga tacataactt gtgtgtatct
      181 ttccgccctc aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca
      241 tttttacggt caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc
      301 tccaggagaa atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta
      361 gtgcctggcg caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg
      421 cgggcgtgct gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca
      481 tggctatcct tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac
      541 tgtctggtaa aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg
      601 gcgcaggtaa actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt
      661 atagcgtggc aatcaccaat accgttgaaa gcttcatgtc tcaccagctg ggtatgacgc
      721 caccgccgcg tgcgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct
      781 tcggtgagca gatgatcgtt aaagcgatga gtattctggt attcccgttt gttggcgtac
      841 tgatgctgct ggctctgtac ctcatcccgc agtggaacgg cgcagcactg gaaacgctgt
      901 ctctggacac tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc
      961 cggtaatggt gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc
     1021 gtgaagagta cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca
     1081 tcatgatggt gctgaccgta atgttcttcg tcttcagctg cgtactgagc ctgactccgg
     1141 cagacctggc tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact
     1201 ttaacgcacc ggttatcgcg tggatggctc cgattatcgc gattatcgct atcaccaaat
     1261 ccttcctcgg ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc
     1321 tgcgtggtaa aggtaagtct atcgaaatca acaagctgaa ccgtatcact gcgctgttca
     1381 tgctggtaac gacctggatt gttgccaccc tgaacccgag catcctgggt atgattgaaa
     1441 ccctgggcgg cccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga
     1501 aagtaccggc aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg
     1561 gtctgattgc aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct
     1621 ttcgggcggc gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata
     1681 ttttcaaaat cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta
     1741 aacaatttac cgacgatctg attgcccgta atctgcttaa agacgtgacc cgcgtggtgg
     1801 ttgacgtgta cggctcgctc tctctgaccg gtaaaggcca ccacactgat atcgccatta
     1861 ttatgggcct ggcgggtaac ctgccggata ccgtggatat cgattccatc cctggtttta
     1921 ttcaggatgt gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt
     1981 tcccggttga tcagtgcatg aacttccatg cagacaacct ttctctgcat gaaaacggta
     2041 tgcgcattac cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg
     2101 gcggtggctt tatcgttgat gaagagcatt ttggccagca gaatagcgca ccggttgaag
     2161 ttccttatcc gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt
     2221 cactctctgg cctgatgatg aaaaatgagc tggcgctgca cagcaaagaa gagctggaac
     2281 agcacctggc gaacgtatgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg
     2341 aaggcgtgtt gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc
     2401 tggtcagcca ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt
     2461 ttgcattggc agtgaacgaa gagaacgctg ctggcggacg cgtggtgact gcgccgacta
     2521 acggtgcgtg cgggattatc ccggcagtgc tggcgtacta cgacaagttt atccgcgaag
     2581 tgaacgctaa ctcactggct cgttacctgc tggtagccag tgccattggt tctctttata
     2641 agatgaacgc gtcaatttct ggtgctgaag tcggctgcca gggtgaagtt ggtgtggcgt
     2701 gctcaatggc ggcggctggt ctggcagaac tgttaggtgc aagcccggcg caggtgtgca
     2761 tcgcggcgga aatcgccatg gagcacaacc tcggtctgac gtgtgacccg gtcgccggac
     2821 aggtccaggt gccatgcatc gagcgtaacg ccattgcggc agtaaaagcg gtgaacgccg
     2881 cacgtatggc gctacgccgt accagcgagc cgcgcgtctg cctcgataaa gttatcgaaa
     2941 ccatgtacga aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct cgcggcggcc
     3001 tggcaatgaa gatcgttgcc tgcgattaat cgctctccag aggcctcgtt ttgcgaggcc
     3061 tcttcccgat ttctcatcca gccgtagcct gttccggcat cgaatgttac ccttatcgcc
     3121 tgatctttaa gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta
     3181 ttcgtcgcat tcatgccgtt cagggatcgc cctgtgtcga aacctgccag catgcgctcg
     3241 atcagctcat tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc
     3301 gcagtagcgg ctggcgtcat cagcgtttac cagattacaa agcgggtcga ccgccaatgc
     3361 cggaagagtt gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc
     3421 cgtgctggtc aaccagcggc aacgaagccg atgacttagc cgccacgctg gcggtcaaag
     3481 tgacacaggc cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac
     3541 tttcaccgac attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta
     3601 tcgataaaga atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga
     3661 tcagcagttc aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc
     3721 tggtcgagtt tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt
     3781 ggcgcaaaaa attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct
     3841 tacaaaccga tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc
     3901 gagccggata cgccgcaaac gtcgtatccg gcattatcac atcagcgcat ttaccaggcg
     3961 gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc accggtacaa
     4021 acatcatcca gtgccgcctg cgccagtgcc ggaatgtctt ccttgcgtac accaacatca
     4081 cgcaaatgtg gcggaatacc gacatcacgg ttgagagcaa acaccgcttc aacagcggca
     4141 ttacgcgcct cttccaggct cataccttcc actttcacgc ccataacgcg cgcgatatcg
     4201 cggtacttct caccggtaaa gtcagcgtta tagcgcatga catgcggtag caggatggcg
     4261 tttgcaacac cgtgtggagt gttataaaac gcgcccagtg gatgcgccat accatgcacc
     4321 aaccctaacc caacattcga gaagcccata cccgcaacat actgcccgag cgccatttct
     4381 tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat gatttcaatc
     4441 gctttaatgt gcagtgcatc ggttagcgcc cacgcgccac gggtaatata cccctcaata
     4501 gcatgagtga gcgcatcgac acccgtcgca gctttcagcg ctggaggcat accatccatc
     4561 atgtcagcgt caataaacgc cacctgcggg atatcatgcg gatcaacgca aacaaacttg
     4621 cgccgttttt cttcgtcact gatcacgtag ttaatggtca cttctgccgc agtgcctgct
     4681 gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag tccttccagg
     4741 ctacgcacat cggcaaactc cgggttgttg ctgataatgc caatcgcttt acaagtatcc
     4801 tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg gaatacaccg
     4861 agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta aatcgcccat
     4921 gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca ttgcaccagc
     4981 gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc atcggttaaa
     5041 gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct gttagccatc
     5101 atccttctcc ttgttgcttt acgaaattac tcttcaattc gtaacccata ggttttgaat
     5161 ttctccagca ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc cgtaatcgcc
     5221 agggtcgtca ggtaaagttg cgccagcact tcaacttcat gcgccagcca taacgctttt
     5281 tccagattca cctcacaagc gataagccca tgatgttgta acaaagttgc cttacgattt
     5341 ttgagagcca gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc ataaggcgcg
     5401 caaggaatag aattaccacc tgccgccgca atcatgtagt gaatagcggg gatcggtcgg
     5461 ttaagaatgg aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc gttggcatcc
     5521 ggtctgcttt gataggccgc catatggaaa cgccattcgc ttgaggggag ctttccttcc
     5581 tcatgtttac cgttgccatc aataaagaca atatgcgact ccgtcagttt ttcatatgga
     5641 atgcctgtag gcgtaatcag catcccatcc tgataacgta cactgacgtt ccccgctgtc
     5701 ccctggttca gtcccaggcg ggtcatttcc aggcaagtgt caataatctg acgagcaagt
     5761 ttatttcgtt ccattagcta cctctctctg attcaaaaca gggcaataat gttgttcctt
     5821 tcacactatt gaattagccg tttaattacc caccatcttc ttcctgatta acaagaaaga
     5881 aattcacaag cttatatttt gtgacctggt tcaactaatc acagtaaata actgcaagtt
     5941 ctctttttat aaccccatta aaaatgaccg ctcttaaaaa tatttatcaa aacggtcatt
     6001 tttctattca cccaagccag gaatgcccgt tttcggcata aacaattaat acggtcatct
     6061 ggtttgtgtt ttttatgatt tattttctga aacgggcatg aaatttcgat tattaaagtg
     6121 atggtagtca cataaagtca ccttccagct aataagtgtg accgccgtca tattacagag
     6181 cgttttttat ttgaaaatga atccatgagt tcatttcaga caggcaaata ttcactgata
     6241 tgaagcccga actcgctggt tttgcacttt tgaaaacata accgattacg tgcttaagct
     6301 tctgaaccta agaggatgct atgggaaaca catcaataca aacgcagagt taccgtgcgg
     6361 tagataaaga tgcagggcaa agcagaagtt acattattcc attcgcgctg ctgtgctcac
     6421 tgttttttct ttgggcggta gccaataacc ttaacgacat tttattacct caattccagc
     6481 aggcttttac gctgacaaat ttccaggctg gcctgatcca atcggccttt tactttggtt
     6541 atttcattat cccaatccct gctgggatat tgatgaaaaa actcagttat aaagcaggga
     6601 ttattaccgg attattttta tatgccttgg gcgctgcatt attctggccc gctgcagaaa
     6661 taatgaacta caccttattt ttagttggcc tatttattat tgcagccgga ttaggttgtc
     6721 tggaaactgc cgcaaaccct tttgttacgg tattagggcc agaaagcagt ggtcacttcc
     6781 gcttaaatct tgcgcaaaca tttaactcgt ttggcgcaat tatcgcggtt gtctttgggc
     6841 aaagtcttat tttgtctaac gtgccacatc aatcgcaaga cgttctcgat aaaatgtctc
     6901 cagagcaatt gagtgcgtat aaacacagcc tggtattatc ggtacagaca ccttatatga
     6961 tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa ttcccggcat
     7021 tgcagagtga taatcacagt gacgccaaac aaggatcgtt ctccgcatcg ctttctcgtc
     7081 tggcgcgtat tcgtcactgg cgctgggcgg tattagcgca attctgctat gtcggcgcac
     7141 aaacggcctg ctggagctat ttgattcgct acgctgtaga agaaattcca ggtatgactg
     7201 ccggctttgc cgctaactat ttaaccggaa ccatggtgtg cttctttatt ggtcgtttca
     7261 ccggtacctg gctcatcagt cgcttcgcac cacacaaagt cctggccgcc tacgcattaa
     7321 tcgctatggc actgtgcctg atctcagcct tcgctggcgg tcatgtgggc ttaatagccc
     7381 tgactttatg cagcgccttt atgtcgattc agtacccaac aatcttctcg ctgggcatta
     7441 agaatctcgg ccaggacacc aaatacggtt cgtccttcat cgttatgacc atcattggcg
     7501 gcggtattgt cactccggtc atgggttttg tcagtgacgc ggcgggcaac atccccactg
     7561 ctgaactgat ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt ttccgttctc
     7621 aaacggcaac taactgaaca tattttccga ataaagtgag gaatctgtaa tgaaaaaaat
     7681 cagcttaccg aaaattggta tccgcccggt tattgacggt cgtcgcatgg gtgttcgtga
     7741 gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg gccgcactgc tgaccgagaa
     7801 actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct gtatcgcggg
     7861 tatggctgaa gccgctgctt gcgaagaaaa attcagcagc cagaatgtag gcctcaccat
     7921 tacggtaacg ccttgctggt gctatggcag tgaaaccatc gacatggatc caacccgccc
     7981 gaaagccatt tggggcttta acggcactga acgtcccggc gctgtttacc tggcagcggc
     8041 tctggcagct cacagccaga aaggcatccc ggcattctcc atttacggtc atgacgttca
     8101 ggatgccgat gacacatcga ttcctgccga tgttgaagaa aaactgctgc gctttgcccg
     8161 cgccggtttg gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg gcggcgtttc
     8221 gatgggtatc gccggttcca tcgttgatca caacttcttt gaatcctggc tgggaatgaa
     8281 agtccaggcg gtggatatga ccgaactgcg tcgccgtatc gatcagaaga tttacgacga
     8341 agccgaactg gaaatggcac tggcctgggc tgataaaaac ttccgctatg gcgaagatga
     8401 aaataacaaa cagtatcaac gcaatgccga gcaaagccgc gcagttctgc gcgaaagttt
     8461 actgatggcg atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg ccgatattgg
     8521 tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg ggcttccagg ggcaacgtca
     8581 ctggaccgat caatatccca atggtgacac cgccgaagcg atcctcaaca gttcatttga
     8641 ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc ttaacggcgt
     8701 ggcaatgcta atgggtcacc agctcaccgg caccgctcag gtatttgccg atgtgcgtac
     8761 ctactggtca ccagaagcaa ttgagcgtgt aacggggcat aaactcgatg gactggcaga
     8821 acacggcatc atccatttga tcaactcagg ttctgctgcg ctggacggtt cctgtaaaca
     8881 acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc agcaagaggc
     8941 tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc cacgaatact tccgtggcgg
     9001 cggttactct tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga ctcgtgtcaa
     9061 catcatcaaa ggcctgggac cggtactgca aatcgcggaa ggctggagcg tggaattgcc
     9121 gaaggatgtg catgacatcc tcaacaaacg caccaactca acctggccaa ccacctggtt
     9181 tgcaccgcgc ctcaccggta aagggccgtt tacggatgtg tactcggtaa tggcgaactg
     9241 gggcgctaac catggggttc tgaccatcgg ccacgttggc gctgacttta tcactctcgc
     9301 ctccatgctg cgtatcccgg tatgtatgca caacgttgaa gagaccaaag tgtatcgtcc
     9361 ttctgcctgg gctgcgcacg gcatggatat tgaaggccag gattaccgcg cttgccagaa
     9421 ctacggcccg ttgtacaagc gttaatacat tactccctga tgtgatgccc ggtcgctccg
     9481 gctaccgggc ctgaacaagc aagagtggtt agccggataa gcaatgttat ccggctatat
     9541 tgcaggagcg attatgaaac aagaagttat cctggtactc gactgtggcg cgaccaatgt
     9601 cagggccatc gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa cgcctaatgc
     9661 cagcgatatc gcgatggaaa acaacacctg gcaccagtgg tctttagacg ccattttgca
     9721 acgctttgct gattgctgtc ggcaaatcaa tagtgaactg actgaatgcc acatccgcgg
     9781 tatcgccgtc accacctttg gtgtggatgg cgctctggta gataagcaag gcaatctgct
     9841 ctatccgatc attagctgga aatgtccgcg aacagcagcg gttatggaca atattgaaca
     9901 gttgatctcc gcacagcggt tgcaggctat ttctggcgtc ggagccttta gtttcaatac
     9961 gttatataag ttggtgtggt tgaaagaaaa tcatccacaa ctgctggaac gcgcgcacgc
    10021 ctggctcttt atttcgtcgc tgattaacca ccgtttaacc ggcgaattca ctactgatat
    10081 cacgatggcc ggaaccagcc agatgctgga tatccagcaa cgcgatttca gtccgcaaat
    10141 tttacaagcc accggtattc cacgccgact cttccctcgt ctggtggaag cgggtgaaca
    10201 gattggtacg ctacagaaca gcgccgcagc aatgctcggc ttacccgttg gcataccggt
    10261 gatttccgca ggtcacgata cccagttcgc cctttttggc gctggtgccg accaaaatga
    10321 acccgtgatc tcttccggta catgggaaat tttaatggtc cgtagcgccc aggttgatac
    10381 ttcgctgtta agtcagtacg ccggttccac ctgcgaactg gatagccagg cagggttgta
    10441 taacccaggt atgcaatggc tggcatccgg cgtgctggaa tgggtgagaa aactgttctg
    10501 gacggctgaa acgccctggc aaatgttgat tgaagaagct cgtctgatcg cgcctggcgc
    10561 ggatggcgta aaaatgcagt gtgatttatt gtcgtgtcag aacgctggct ggcaaggagt
    10621 gacgcttaat accacgcggg ggcatttcta tcgcgcggcg ctggaagggt taactgcgca
    10681 attacagcgc aatctacaga tgctggaaaa aatcgggcac tttaaggcct ctgaattatt
    10741 gttagtcggt ggaggaagtc gcaacacatt gtggaatcag attaaagcca atatgcttga
    10801 tattccggta aaagttctcg acgacgccga aacgaccgtc gcaggagctg cgctgttcgg
    10861 ttggtatggc gtaggggaat tcaacagccc ggaagaagcc cgtgcacaga ttcattatca
    10921 gtaccgttat ttctacccgc aaactgaacc tgaatttata gaggaagtgt gaaatgctga
    10981 aaacaatttc gccgttaatt tctcccgaac tattgaaagt gctggcagag atgggacatg
    11041 gagatgaaat tattttttcc gatgctcact ttcccgccca ttcgatggga ccgcaggtga
    11101 tccgcgctga tggcctgttg gtgagcgact tgctccaggc gattatcccg ttatttgagc
    11161 tggacagtta tgccccgccg ctggtgatga tggcagcggt agaaggtgac actctcgatc
    11221 ctgaagtaga acgacgttac cgtaatgcgc tttcactaca agccccgtgt cctgacatca
    11281 tccgcatcaa tcgttttgcg ttttatgaac gggcgcaaaa agcctttgcg atcgttatca
    11341 caggcgaacg agcgaagtac gggaatattc ttttaaaaaa aggggtaaca ccgtaatctc
    11401 ataccggtac gcccgcatga cgcgggcggt tatcgaatga tggggtgaaa aatatgaaag
    11461 cggcacgcca gcaagcgata gtcgacctgc tgctgaacca taccagcctg accacggaag
    11521 ctctctctga acagctaaag gtcagtaaag aaaccattcg tcgcgatctc aatgaattac
    11581 agacgcaggg taaaattctg cgcaatcatg gacgcgctaa atatatccac cgtcaaaatc
    11641 aagacagtgg cgatcccttt cacatccggc tgaaaagcca ttatgcgcat aaagcagata
    11701 tcgcgcgcga ggcgctcgcg tggattgaag aagggatggt gatagcctta gacgccagtt
    11761 caacttgctg gtatctggca cgccagttgc ctgacatcaa cattcaggtc ttcaccaata
    11821 gccatccgat ttgccatgaa ctcggtaaac gcgaacgcat tcaactgatc agttccggcg
    11881 gcacacttga gcgcaaatat ggctgttacg tcaatccctc gctgatttcc caacttaaat
    11941 cgctggaaat cgatctgttt attttttctt gtgaagggat cgatagcagc ggcgcactgt
    12001 gggactccaa tgccatcaac gctgattaca aatcgatgct attaaaacgt gccgcgcaat
    12061 cgttgttatt gattgataaa agtaaattta atcgttcagg ggaagcccgc atcgggcatc
    12121 tggatgaggt aacgcacatt atttctgatg agcgccaggt tgcaacttct ttggtaacag
    12181 cctgacggaa agggtagcag gccggagacg acgccccggc cttgcctgtt atcgctcgtc
    12241 gcgacgacca cccaccgcag cccagatgcg gcggacgtgc accgtcactt cttcgcgatc
    12301 gtgatacaac tgccgcgcct gaatctgagc atttatgcca tgttcatcaa gctgtgcctg
    12361 aatatacgcc agattgtgtg atacttcttc gtagcgtttt ttcatcggca gtttgagatt
    12421 gaaaatggtt tcacggcacc agccattaac cagccactgc gccatcaatg ccgcaacttt
    12481 agccggtttt tcaaccatat cgcataccat ccaggagata ttgctgcgtg tcggacggaa
    12541 tttgaaaccg tcttcccgca gccacgtcac ctgcccggta tccatcagac tttgcgccat
    12601 cgggccgttg tcgacggaat aaacccacat gttgcgcttc accagttggt aggtccagcc
    12661 gcctgggcaa gcgcccaaat ccaccgccca catcccgttc gccaggcgtt catcccactc
    12721 atccgcagga ataaacacat gaaatgcctc ttccagtttg agtgtggaac gactcggcgc
    12781 atctgccgga aatttcaggc gcggaatgcc catatagaac ggcgaattat tgttgctgta
    12841 tgagtaaccg gtatagcagc aacctggtgc aatgaagaat acatgcacaa ccggacgctt
    12901 cggcgtttca tagttcgcca gcaccccggc atcgcgcaag gcagcgcgta gcggcacggt
    12961 gaatttacgg cagaatttca gcaactcttt gctttcgttg gtatcggcaa cttcaacacg
    13021 cagttcaccg cccttctcta ctacgccctg taacatgccg acaatggggg taatacgatc
    13081 ttctggcggc aaatgctgca ggagttcccc caccacaaac cactggcggg caaaaattaa
    13141 tgaactgaac ggcagctcac ggattaactt atcgccatca tcaggttgat aacattcata
    13201 aatgacataa cccgcattct ctttcacgcg ggcaaaaccg aaaatttccc gctggccggc
    13261 tttatcggta atttctgcgg cgcactcttt ttcaaagccc ggacggcaca gcaatacaac
    13321 cttattcatg acttacgccc ttgcgcttta aacggatagc accaactaac attaacgccc
    13381 agcccgcaag gaaactcacg ccgccaaccg gtgtgacaaa cgcccacaaa cgcagatggg
    13441 acagtgccag gcaataaagg ctgccgctga acagcaccgt gcctaacgcg aggaaaacgc
    13501 tactccagta aaaccagata ctgatgcgac gctgcattgc caccgccaga cctaagatcg
    13561 ccagcgtatg aaacgcctgg tattcgaggc cggtctggat ccagcccatc tcaacggcgc
    13621 ccatggtttt acttaacaca tgcgcgccaa aagcgcccag agccacaaaa atgaagccgc
    13681 taatggcggc gaaaatcagc ataaaacggc tggtcatggt cgtaccctac gtaaattatt
    13741 gttcataacg aaagcggaat ttttcttgtt cagcagcggc tttcgccagg atccattggc
    13801 gaaaggcggc tattttaccc agttctgcct ggctgtcatg acaaaccaga taaaaagcgt
    13861 ttttactgac cagaacatca ttaaacgggc aaacaagacg tccggcttca atttcagatt
    13921 gcgccatcac gttatttgcc agcgccactc cctgcccgtg aatagccgct tgcagcacca
    13981 tggcgctatg gctaaaaatt ggcccttgct gaacgttgat atgatttaac cccaattgtc
    14041 gggtatatgt ctgccagtca cggcgcgaag catcatgtaa taacgtatgt ttagccagat
    14101 cttccggggt cttcaagggt ttttcgccag tcagcagtag cggcgaacac accggcaata
    14161 aatattcggc gtaaagtttt tccacccgta gccccggcca gttgccccga ccataaaata
    14221 tcgccacatc aacatcatcc gccagcttat cttcctgacg atcaaccgcc tggattcgaa
    14281 cgtcaattcc cggataagct gaattaaagc tggaaagtcg cggaaccaac caatgaatgg
    14341 cgaaactggg gagtaaactg accgtcaacg cccctttggc gctacgggcc tggagtttac
    14401 gcgtcgcttc ggttaattgc gaaaatatct ctttgatatc gaggaaatag ctttgacctt
    14461 cctcggtcag caggagtgaa cgattacggc ggcggaacag ttttagcccc agaaaatcct
    14521 caagagactt gatttgatga cttactgcgg cctgggtcac aaaaagctct tctgctgcgc
    14581 gagtgaaact taaatggcgt gctgcggcat caaaaactcg taaggcattt agcggtggta
    14641 atcgtttaga catggctttt aaactttgat gttaaatgaa tttaacaatt agatcacact
    14701 gtgtaaccta ttagtttttt taatctgagc cattataaat tgtccgttga gcttctacca
    14761 gcaaatacct atagtggcgg cacttcctga gccggaacga aaagttttat cggaatgcgt
    14821 gttctggtga acttttggct tacggttgtg atgttgtgtt gttgtgtttg caattggtct
    14881 gcgattcaga ccatggtagc aaagctacct tttttcactt cctgtacatt taccctgtct
    14941 gtccatagtg attaatgtag caccgcctaa ttgcggtgct tttttttacc ttgcgatcgc
    15001 gaattactga tccagttcga ccatctcttt cacgtcggta cggttgatct gctgtttgtt
    15061 gccattagcg tctttatacg aaatcatacc ggtatcgtta tcagtctgtg gtttgccgtc
    15121 agagacgatg gtacgtccgt cattggtgtg catcacatag ttcgaaccgg aacaggcgct
    15181 cagggcaaaa gtcagcatac aggcagaaat aattgcggca gtctttttca tcatcttctc
    15241 cttaaagcga ttaataaatc ttaatagtat tgattaacag gctaaaatta acgcctaaca
    15301 ctattcagca tatgttactg acgcggcttc gccaggatat ccagataatt ctgatggtta
    15361 gcactctcct tgtatcaaag tgaattttgc gtcacgatcg gtgcatcaag ccgaggagta
    15421 ccatgaacgt ttttaatccc gcgcagtttc gcgcccagtt tcccgcacta caggatgcgg
    15481 gcgtctatct cgacagcgcc gcgaccgcgc ttaaacctga agccgtggtt gaagccaccc
    15541 gacagtttta cagtctgagc gccggaaacg tccatcgcag ccagtttgcc gaagcccaac
    15601 gcctgaccgc gcgttatgaa gctgcgcggg aaaaagtggc gcaattactg aatgcaccgg
    15661 atgataaaac tatcgtctgg acgcgcggca ccactgaatc catcaacatg gtggcacaat
    15721 gctatgcgcg tccgcgtctg caaccgggcg atgagattat tgtcagcgtg gcagaacacc
    15781 acgctaacct cgtcccctgg ctgatggtcg cccaacaaac tggagccaaa gtggtgaaat
    15841 tgccgcttaa tgcgcagcga ctgccggatg tcgatttgtt gccagaactg attactcccc
    15901 gtagtcggat tctggcgttg ggtcagatgt cgaacgttac tggcggttgc ccggatctgg
    15961 cgcgagcgat tacctttgct cattcagccg ggatggtggt gatggttgat ggtgctcagg
    16021 gggcggtgca tttccccgca gatgttcagc aactagatat cgatttctat gctttctctg
    16081 gtcacaaact gtatggcccg acgggtatcg gcgtgctgta tggcaaatca gaactgctgg
    16141 aagcgatgtc gccctggctg ggtggcggca aaatgattca cgaagtgagt tttgacggct
    16201 tcacgactca atctgcgccg tggaaactgg aagctggaac gccaaatgtc gctggtgtca
    16261 taggattaag cgcggcgctg gaatggctgg cagattacga tatcaaccag gccgaaagct
    16321 ggagccgtag cttagcaacg ctggcggaag atgcgctggc gaaacgtccc ggctttcgtt
    16381 cattccgctg ccaggattcc agcctgctgg cctttgattt tgctggcgtt catcatagcg
    16441 atatggtgac gctgctggcg gagtacggta ttgccctgcg ggccgggcag cattgcgctc
    16501 agccgctact ggcagaatta ggcgtaaccg gcacactgcg cgcctctttt gcgccatata
    16561 atacaaagag tgatgtggat gcgctggtga atgccgttga ccgcgcgctg gaattattgg
    16621 tggattaatg acaaacccgc aattcgccgg acatccgttc ggcacaaccg taaccgcaga
    16681 aacgttacgc aataccttcg caccgttgtc gcaatgggaa gataaatatc gccagttgat
    16741 catgctgggg aaacagcttc cggctttgcc agacgagtta aaagcgcagg ctaaagagat
    16801 tgccggatgc gaaaaccgcg tctggctggg atataccgtg gctgaaaacg gtaaaatgca
    16861 cttctttggc gacagcgaag gacgtatagt gcgcggcctg ctggcggtgc tgttgactgc
    16921 cgttgagggg aaaaccgccg ccgaattgca ggcacagtcg ccgctggcat tgtttgatga
    16981 gctgggattg cgtgggcagc ttagcgcctc acgcagccag gggttgaatg cgttaagcga
    17041 ggcgattatc gctgcgacga agcaggttta agcacagaac cgggtcggat aagacgttcg
    17101 cgtctcatcc gacctgattg tttaaccctg acgcgccgct ttcgccatca tcttcttcag
    17161 cgcatgagaa accgcaacaa acccaaaggt ggcggtcacc atcgttgccg cgccaaatcc
    17221 agatgcacaa tccatccgct tcggcccttc tgccgtggct ttcatcgcac acaccgtacc
    17281 gtctgactgc gggtacacca gcgcttcagt agaaaacacg caatccacgc cgagcttacc
    17341 tttactgttt ttcactacgc caaaatcgct tttcaggcgc tcgcgcaact tcgccgccag
    17401 cgggtcctga atcgttttcg ccagatcggt aacctgaatc tgcgtcgggt caatctgccc
    17461 acccgcgcca ccggtcgtga ccagcgggat tttattgcgc cgacaataag caatcagcgc
    17521 cgctttgggt cgtacactat caatggcatc aatcacgtac gaataaccgg cactcatata
    17581 ctgcgctacg ttatccggcg tcacgaaatc atccaccacc gttacacggc actccgggtt
    17641 aatctggcga atacgttccg ccatgacttt cgcttttgcc agcccaacgt tatcgcgcag
    17701 ggcatgaatt tgccgattgg tattggtgac gcacacatca tccatatcga taagcgtgat
    17761 tgcgccaatc cccgtgcgcg ccagcgcttc cgccgcccag gaaccgacac caccgatacc
    17821 gaccacacaa atatgcgcgt cagcaaacag ttgcaacgct ttttcaccat acagacgcgc
    17881 tgtgccacca aaacgctgac gccatgcatc actaattacc acagacatag cacctcagaa
    17941 ttaaaaaggg tgaggttttc cctcacccta aacgcataaa tgcctgatgc gctacgctta
    18001 tcaggcctac ataactcctg caatattttg gatttgcatg atttgtgggc cggataaggc
    18061 gttcacgccg catccggcat gaacaacgcg cacttagtca ataatcagga tgtgaggtta
    18121 cccctcaccc tgtcatatcc gtaaaaacgg catacagaat atcacatcag ccgctaaaga
    18181 cgttacctgc gcccggggcg gttttcagca cccagacacg tccatagtgg ttgtaccaac
    18241 ctgcgcggtg tccggcttcc ggcccgatcc cttgatagat atcgaagtgt tggcctttga
    18301 ttgcgccccc gacatccaac gccaccatca gacgcaattc gtactgaccg ttaaatttgc
    18361 cgttattatc cagcagcggc acttctgcca gcaaggtagt acctggcgga ataattgaac
    18421 gatcggaggc aacagaagca cggccaacca gcggcaccgc acttgcccct ttcaccggtg
    18481 caaaagattg cggtttaaag aagacgaaag acgggttctg ttccagcagc tcgcgaacct
    18541 cggcttcact gtgtgtttcg ccccaatgac gaatcgcctg catcgacata tcttcttttt
    18601 tcacttcgcc acggtcgatc agcaccttac caatgctgcg ataggcatga ccgtttttcc
    18661 ctgcatagct gaaaaagtta agcggactgc catcaccaaa gtcgatatac ccgctaccct
    18721 gcacatccat aatgaagtta tccatcaggg agttgctgta agcgagaata tatttatcac
    18781 tcagcgcccc tgcgtagatc tccgcacgag acggcagacg accacgtttt ggcggcatac
    18841 ggtaaatcgg atactggaac tcgccctggc gggtatggcg cgcctgaatt accggcgtgt
    18901 aatagcctgt aaactgcacg ttaccatagt tgtcggcacc ttccatctgc caggcatcaa
    18961 tgccgaactg acgcatattg cgggtatcac cgcctgcgcg cagccactct tgcaccgcgt
    19021 tataaacatt actctggttg ccatacagac gcggtgacga attacggata tggttaattt
    19081 gctcggcaaa atcaccggcg ttaatcggcg cgccaacggc atctggctgg ttcaccagag
    19141 agaaaggctg ggtaaatttc ccgtctttat attgctgtcc gcgatcggtt ggtttggaag
    19201 agcaggcggc aagcattgcc acaaccgtgc ccataagaag gtactttacc caacgtcctt
    19261 tcattgttct cttcttcagg ttaaaaaata aggcgcaacg aagataacaa accgccgcgt
    19321 ttaatgaaat gagcagcctc tccctgacgc aaattttgca caaaaaatag gctttagtga
    19381 tttgtttttg ttcaaaatca tgccaaatcc gtgatcgggg taaaaaaaag gttgcatgaa
    19441 aacgcgagcg gagtatagtg cgcatccacg gacgcggggt ggagcagcct ggtagctcgt
    19501 cgggctcata acccgaaggt cgtcggttca aatccggccc ccgcaaccaa ttattgaaca
    19561 ccctaacggg tgtttttttg tttctgctct cccataaaaa aagcgccatt cagcgccttt
    19621 ttatcatccc cttctcgcca gcgtcgcccc atcggcaaaa tacgctttaa ttcccgcaag
    19681 aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct cttcctcaac
    19741 gttactgata aacgccgttt cgaccagaat ggagggaata tcaggcgctt tcagcacggc
    19801 aaacccggcc tgttcaacct gatttttatg cagcttgttg attttaccga gcttattcag
    19861 caccgcttta ccaaacttaa ggctgtcggc aatggtcagc gactgcacca tatcgaacat
    19921 ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt ccgaggcgtt
    19981 ctgggtctgt gccagatatt ttgccgcagt actggttgcg cctttggttg agagcgcaaa
    20041 cactgaggaa ccgctcggct ggcgactggt aaaggcgtcg gcatggatag agacaaacag
    20101 gtcggcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga agatgtcttc
    20161 attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc gcagacggcg
    20221 agctatttgc aataccacgt ctttttcgcg cgttttgtat ttccccaccg caccggagtc
    20281 ttcgccgccg tggccagggt caagcataat aacaatagga cgatcgcgcc ctgctttacc
    20341 cggttgtgga ccactttgtg ctggcggcac ctgcttttcg aggtcgcctt tgttgtaatc
    20401 ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat agaggtccat
    20461 caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg gttttacgtt
    20521 ttgctttaat tcaaaaacca tccgtacggt ctgcgggtca aattgcccga cgcgcgccga
    20581 cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta ccgagttcag
    20641 gtttacatct tcgatatcca ccaccacgcg ttccgggtta ctcaacgcga actgcttata
    20701 tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag gccagacgcg
    20761 caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca atagccacat
    20821 agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc ctgacatgcc
    20881 tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt gaccggaaac
    20941 tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt ttatattcac
    21001 ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc tgcgaaaaaa
    21061 cagaataaaa atacaataat ttcgaataat aatgcaaagg ggtgtgccgt ggtaaaggaa
    21121 cgtaaaaccg agttggtcga gggattccgc cattcggttc cctatatcaa tacccaccgg
    21181 ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa tttctccagt
    21241 atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt ggtctatggc
    21301 gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct gtatcacaag
    21361 aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc gggaacattg
    21421 caactggata ttactgctcg cctgtcgatg agtctcaata acacgccgct acagggcgcg
    21481 catatcaacg tcgttagtgg caattttatt attgcccagc cgctgggcgt cgatgacggc
    21541 gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat ccatcgtcaa
    21601 ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac tggcgagagc
    21661 tttaatctga cctcggaaga gattgccact caactagcca tcaaactgaa agctgaaaag
    21721 atgattggtt tttgctcttc ccagggcgtt actaatgacg acggtgatat tgtctcagaa
    21781 cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg cgattacaac
    21841 tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg cgtgcgtcgc
    21901 tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt ctcacgcgac
    21961 ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc aacaatcaac
    22021 gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat tctggtgcgc
    22081 cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca gcgcgataac
    22141 acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg ggaaatggcc
    22201 tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct gctggaacgc
    22261 attaccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac cacgcgcagt
    22321 attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact gcccgagagc
    22381 aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga tttagggtaa
    22441 gcgatgaaaa ttgtcggatg cgacatgcgt aacactcgtg cgtcgcatcc ggcaattacg
    22501 tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta cgagtggcga
    22561 ttgccgcact taatatacgc tcatcggcat acagcgacag acggcgacgc gcgcgggtca
    22621 ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt aaaatcaacg
    22681 ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac gtagtttcgt
    22741 gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc gcaaaccaga
    22801 cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac aacccaagcg
    22861 cgctgtcatt acgggcaatc attaccggtc gaccttcgta ccaacgagag tgcggatgac
    22921 gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt ccagccacgc
    22981 caaacggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc tgaatgatta
    23041 aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc gcaagagctt
    23101 cctcaagcat cgcaatataa tcttctccgc tctgtaaaag ccgtttttcg atatcagtaa
    23161 aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc gccgcagcta
    23221 actgaccaat gccagaatca ctgccgaaac gatagctttt ttgcagcagg cagagactgt
    23281 cgcgcaaaga tgccgcttct gtgccagttc ctgccggaac gtgagttccc gtcaggcggc
    23341 ttagctgccc ggcacgctcg gcggtaaagc cggcattggc ataagcgcag atatcgccca
    23401 gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag atcactcgcg
    23461 catgatcggg caaggcgtcg atcagtctcg acatcatagg cagatcgatc attgacgctt
    23521 catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt aaacgctggc
    23581 tacccggctg cgcacccagc aatcggtgca aagtactggc atcttccgga atgcgtttct
    23641 tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg gttaagcgcg
    23701 cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg tcggccattt
    23761 gaattaacgc tgccagtaac ttcgccacgg tggtcgtttt accggtgcct gggccgccgg
    23821 aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc cagttaattt
    23881 catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca tcaacctcaa
    23941 tggcatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac cacatgcgat
    24001 tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc ctgctgaccg
    24061 cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca ctgacacagg
    24121 tcgccaacag cggatgcgac gcctcgttat tttccagtcg tgaaagcggc aaacagacgt
    24181 gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca ggatgttcat
    24241 ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg tgctccacag
    24301 cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac cggcaaacat
    24361 ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc cttgttgcgg
    24421 atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt gacgatcata
    24481 gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat aaagctgata
    24541 ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg tgtaagccga
    24601 actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc cttcgtggcg
    24661 gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact ccagcggcgg
    24721 gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc tggcgatgag
    24781 cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt cggaaagctg
    24841 actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga tccactcggt
    24901 caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct gtacccagtt
    24961 cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga atgtccccgg
    25021 tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga cgctgaccac
    25081 gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt gaccacgctg
    25141 ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt gtagtgtcct
    25201 cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat caccagtttg
    25261 tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc gaagccctgc
    25321 cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt ggacgtcggt
    25381 gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac tgcaatgcca
    25441 aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac gttcgacctc
    25501 cgcgaggtcg acgctttctg gcgcagcgtt aagatccaaa actgcctcaa acgagtggcg
    25561 atcgtgataa aacgcctgat cctggacgcg gaaattggtg ataaacggca gccagaccaa
    25621 tggatattcc agccctttcg atttgtggat cgtgacaatc tgcaccagat gtttatcact
    25681 ttcgagacgc atttgttggc tggaggcatt actgtctggc tcgaggatat gttgcgataa
    25741 ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgta gcagttcgct
    25801 gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca agttttcagc
    25861 aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt tgcgccagat
    25921 ttgccgataa ccatcgaact cttcgactac cacatcccac gcatgttcgt cattgttcag
    25981 cgtttcgata tccagcgcgt tcagccccat cattgacgtt gccagcgcac tacgcagggt
    26041 gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct gcgcttccag
    26101 agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca gcaacgttaa
    26161 ggcgtcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacactga tgtccgaagc
    26221 acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct gtccggcttg
    26281 tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat agtcgccaac
    26341 gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt cacctttaaa
    26401 cacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg gtatttcgcg
    26461 aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acactgttca ccattcctgg
    26521 tgcggaacgc cagttggtgt ctaaagtgta gtgggcgtga acttcgctac gcgccttcat
    26581 ataagtgaag atatccgcac cccggaatgc atatatggcc tgtttcgggt cgccaatcag
    26641 caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt actgctgggg
    26701 gtcggtatcc tgaaattcat caatcattgc caccgggaat cgcgtacgga tcgccgctgc
    26761 caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca tgtcatcaaa
    26821 acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct cagccaatgc
    26881 gcgggttatc accagatcac ggatcgacaa tggttctgca agcagttgtt cgatcgcctc
    26941 aaacagtgga tgtcgcgggg tttccccccc ggctttcgtg cgatcttcta agaaacgctg
    27001 agagaatttt tccagcgact ccggtaactg ataactgttg gtctcttctt ctgcccaggc
    27061 gctgatcttc tcgatccatt tagcctgatt gctacggtta aacttgcgtc gatcaatacc
    27121 agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct gttttaccgt
    27181 atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat cgggcggcgg
    27241 tgctttgata accggtgctt cgccttgcag ataacgatta atatcgcgca gcaacgcctg
    27301 cggccctttc caggtttcaa agaccaccag ggcaatttca cgcggcagcg ggtagcagtg
    27361 gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt caatcagctg
    27421 ctgctcaaac agcataccgg attcaaaggc attcaggttg agcatgcgct ggcaaaaacc
    27481 gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca accactgcgc
    27541 ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat tgtcggtggt
    27601 ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac cgcgcaattc
    27661 tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca gcgggcgggg
    27721 aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg caatcgtaaa
    27781 ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg gcaagcgcaa
    27841 aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc attgtttgct
    27901 gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat tcggctttcc
    27961 cgttctggct gccggaaatc tgcgacagga tagccatgcc ttgcggctcg accaccgcct
    28021 gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc acgattttat
    28081 cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc gatgcttctt
    28141 cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg atttgcgcaa
    28201 actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac gccttgtaac
    28261 gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc atgccccact
    28321 gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct tcggtacgca
    28381 actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag gctgagctgg
    28441 tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg gtgctgttac
    28501 cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg tttcgacacc
    28561 actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt gttgcctggg
    28621 cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt aaggcgtcgc
    28681 gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt tcatctcgcg
    28741 agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca aacgctttac
    28801 ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca agctgatctt
    28861 ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc ggcagacgct
    28921 gggtgtaacc attagcatta accataaggc cgttgttagc gttggtggaa aaacttatgc
    28981 caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga taatcattga
    29041 gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc aaaatcaggc
    29101 tgacatcagc tttgggttcg ctggaaaaat aacggcttgg cgcatacacc acgcgcagat
    29161 tcgactcatc aacaatcagc tctggatggt catatttctt ctctgactta atcagcgaga
    29221 aatcgtcagg aatatagggg ttaagctccg gtaaggagag cgcaatattg gcagcttttt
    29281 gctgccagtc agcgaaagtt tgttcgctga ttttatcgac ctgatacggc gcatcgacaa
    29341 aataagccgt tttattgtgc ggctctttcg ggctgatata ccagatacgc gcattctgcg
    29401 gcgtcatcat cgccagacgt tcttttactg ctttagcatc gtaccgatcg gcaatattga
    29461 ctgcatccag cgtatgctca accggaacgc gaatcatggt atctgccagc cattcgacgt
    29521 aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca ttcgccagtt
    29581 catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag ctaaaaattg
    29641 ccgccacaac ctgatcgcga ttagccaggc ctttatcggt taaagacgca gagatcgcta
    29701 atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc tcaactaatc
    29761 cctgcttttg cagccagtcg gaaagtgtac ctgggctgcg attaccaatc agataggtaa
    29821 tcaattcatc cgttttacta cggaacttcg ctgagttgtt atcgatgcga aactcaacgc
    29881 gcagaacttt acgcggcagt gccgggacgt aatgaatgat aatgcccttt tgcgcgtcgg
    29941 tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg cgaccaaagg
    30001 tgtccgccgc catttttgcc aactccggca gcggtttatt gctgtaaata accgccttca
    30061 tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc accggattac
    30121 caggtttatc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg gggtgtgccg
    30181 ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta cgcgccatgg
    30241 ttaattcagc gttcaccgca ttacgctcac gttcggcata tttcttgtcg agcaaaggtt
    30301 cagcaatagc atcggccagg cggtctaccg caccaggcaa ggcgtcgttc tcaacttcca
    30361 gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg tgcattttga
    30421 gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga ctcatatgtt
    30481 caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca acgggcacca
    30541 ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc accataccgt
    30601 tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg atggtttcct
    30661 gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca acaaacaaca
    30721 ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa ctttattttc
    30781 tggccagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg tgctgcacga
    30841 gtcagcctat gtctatatat ccatcagtcc gtgactggtg cgcatcataa tgtaagcgaa
    30901 tgaattgcgc aatttttata cagcactcat gactgattaa agcgaaacag cggtaacagg
    30961 aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg ccgccagagc
    31021 cgttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc gtaagcctga
    31081 aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc atcgttttgc
    31141 gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag caatggcgcg
    31201 gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc ctgttcggct
    31261 gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact ttcaccatta
    31321 ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg ggccacactt
    31381 aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa ccagccagtt
    31441 atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg gcgacaggca
    31501 atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa aatttcacca
    31561 aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg ttcggcatca
    31621 tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct aagcccttcc
    31681 agaataaatg gctcggtgtc ggggatttcg ctgtcttcgg tacggaagtt cacctgcaaa
    31741 cgcatctgga aaaatgcccg caccggatgt gcccagaatc gttgtagcgt ttccagcggc
    31801 acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc tttaccagcc
    31861 tggctggccg caggtagcca ttcacgagca taactttgtc gttcgcctgg ctggtagttt
    31921 tgcggatcaa acggcattcg ggtatgaagg caagtaagat gcgattttac ccttgcctcg
    31981 ctttcatcac agttgagcgc ctcatcgccc ggtagataat gactttgtcc gatgtagtcg
    32041 atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat ggaacgcccg
    32101 atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa cagatagcgg
    32161 tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag gtcaaagccc
    32221 aatggcgcaa gctggcgtgg ataaacgccg tcgttcattc ccagcaggca aaccactttg
    32281 aatgggatgg aacgcattgg catcagagta caaatgttaa ccggtccggc gagaaaacgc
    32341 tgactgatac gttcctgatc caggcgctgt gccagttcat cacgcaatag tgacagcggc
    32401 accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca ttgttgttcg
    32461 atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt gagcatatcg
    32521 cgacaaaccg gcaaccactc ttccagcgga cgctcctgcg ccagcccgcg acgccagatg
    32581 tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa gccgctcgat
    32641 tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc gtagcccagc
    32701 aacatacgcg tcaggccaaa ccgccaggtg tgttgaccgg tggcggggag ttccagctcg
    32761 cgaacgttat cgtcatctat cccccagcga atgccggatt cgttgaccca ctggcgtaaa
    32821 taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac atccagcagc
    32881 gccagcacat cctctgacac aaagcgactg tcaggcagtg ataacaggct gataaacgct
    32941 tccagtaccg gatgtgactg ccgcgcccga cggtcggaaa tggcgtaagg caggtaacga
    33001 tccgcaggtg cgctaccaaa cacagcctga ataaacggac tgtagctgtc gatgtcagcc
    33061 accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cctccagcat cgccagcaag
    33121 cgatcgtgta aaacttcaac ttcacgctgc gggctatggc aaacgtggaa ggtgatactg
    33181 ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc gatgttcaca
    33241 cccgcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg cagcaggttg
    33301 tctggcgtca catcgacaaa agcgtccagc tcctggctgc tctccaggtc agaaaggaga
    33361 taaatgtagt cgcgcccaag cttcccccat gaggccagca gcgggttgcc gacatcctgt
    33421 tcaccatcgc tgttaaagag ctgtccggca ttttcgctat cgcgaaatag cggcaattcg
    33481 cgatcttcaa aactgtgtcg gcgctggcga gtcagtagtt tcgccagata agcaggatct
    33541 ttaatgtcgc cccagtaata acggcagggg ttggtaaaca ggagatggat ttcaatatgt
    33601 ttacccagcg cctgtagcgc ctggagataa acaggcggta acgcggaaat accgcatata
    33661 aagacgcgcg aaggtagccc cggcgggcag gtcgtcgcgg actccagcgt ttcgataaag
    33721 cgctgataga gattggcgcg gtgccagcgc ggttgcccga gttgatgggt atattccacc
    33781 aacgccttcc acaacggcgc ttgccaggcc tgtgcttctc ccagcccttc aaccaaatgt
    33841 cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc aaacaggtcc
    33901 gccgcttttg aagaaagctg gaacagtttt cgcttgtcgc tatcgtcagt cagataatgc
    33961 cgcaacaggg taaagtcttc gcgctccaac aattgcggca gcagagtcat cagtttccag
    34021 ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac ccggacgaac
    34081 atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat accaaacttt
    34141 tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag aatcatctct
    34201 ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa cgcttccagc
    34261 acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta ctgacgattc
    34321 gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag cgtaacgctg
    34381 atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt ggcaggtggc
    34441 gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg gtactggttt
    34501 ctgctggcga gactgttcat taatgttcgc tggatacccg ataacgcagt gacaatcatc
    34561 accatcagca ccatcgcaac cattacctcc ggcaggctaa agccttgttg attcttcagg
    34621 gaagctgaca taacgccctc tctttcagcg gacaaaaatc gctccagccg cgtggcgaaa
    34681 aaacaatgtt tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct tcataaccgg
    34741 caatcaataa ggcttcatta tctgccagta aacgcaaaca aacctgggca tcggttccag
    34801 cgtactgcga gcactgaact gctggctgcg tctgccagga gtgcattttt ccccacgcca
    34861 gcgccgactg aacgatggcc tggcggcgca atgactgact ttccatgctc acgcgagagg
    34921 caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa accagcagca
    34981 tcaggaccag agccagtgac gaaacacctt tttcgcggtt cacaggttga atcctgtcac
    35041 gctatagctg gcatccacca cggtttgcgg ttcagactta ctggcagcac gcatattaac
    35101 cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag tgtcgataat
    35161 gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg tcgcaccgcg
    35221 tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcagact cttttaccgg
    35281 ttcgcgatcc cagataccgt tactgttcgc atcccactgc acaatgatac agtcaccctg
    35341 tccgacaatt tccagccctt cgccggtaca gctgccatga caataacccg ccctctggag
    35401 atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca gcttacgggt
    35461 gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca gcaacaatac
    35521 gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct cttttacagg
    35581 catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga aaccaccagc
    35641 caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt attgcgcagg
    35701 ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca gcgtggcaca
    35761 aagaccaatg gtgaactgcc atgacaggta ttggccccag cagcggaact cacaaggcac
    35821 cataacgtcc cctccctgat aacgctgata ctgtggtcgc ggttatgcca gttggcatct
    35881 tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccatag ccgttgcgac
    35941 tgctgccagt attgccagcc atagagtcca cttgcgctta gcatgaccaa aatcagcatc
    36001 gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc agtatggagc
    36061 gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc ggactattta
    36121 cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta acggagggta
    36181 aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt agatagccac
    36241 cggcgcttta atgcccggat gtggatcgta gccttcaatc tcaaagtctt cgaaacggta
    36301 gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac gcggttcgcg
    36361 gcttaattgc agatgagttt gatccatatg attgctgtac aggtgcgtgt cgccccccgt
    36421 ccagacaaaa tcgcccactt ccagatcaca ctgctgcgcc atcatatgca ccagtaacgc
    36481 gtagctggca atgttaaacg gcaggccgag gaaaacgtca caggagcgct gataaagctg
    36541 gcaagagagt ttgccgtctg ccacatagaa ctggaagaac gcatggcacg gtgccagcgc
    36601 cattttatcc agttcgccta cgttccacgc tgaaacaata atgcggcgcg aatccgggtc
    36661 gtttttcagc tggttcagta ccgtagtgat ctggtcaata tgacgaccat ccggcgttgg
    36721 ccaggcgcgc cactgtttac catacactgg cccgaggtcg ccgttttcat cggcccattc
    36781 gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc cctgcagaaa
    36841 ccacagcagt tcatggatga tggaacgcag gtggcaacgt ttagttgtca ccagcgggaa
    36901 tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc cggttccggt
    36961 acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta aatactgttt
    37021 catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga ccatcatgat
    37081 cacacccgcg acaatcatcg ggatggaaag aatttgtccc atgctgatgt actgcaccca
    37141 ggcaccagta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc gaaacgcgcc
    37201 gtaaccaatc aggaacaaac ctgagacagc tcccattggg cgcggtttac gaatatacag
    37261 gttgaggata ataaacagca ctacaccttc cagcaacagc tcgtaaagct gtgatgggtg
    37321 gcgcggcagc acaccgtaag tatcgaaaat ggattgccac tgcgggttgg tttgcagcag
    37381 caaaatatct tctgtacggg agccagggaa cagcatggca aacgggaagt tcgggtcaac
    37441 gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcaccaa gaccaaacgg
    37501 aatgagtggt gcgataaaat cagagacctg gaagaaggaa cgtttagtac ggcgggcgaa
    37561 gataatcatc accacgataa cgccaatcag gccgccgtgg aaagacatgc cgccgtccca
    37621 gacacggaac agatacagcg gatcggccat aaactgcggg aaattgtaga acagaacata
    37681 accaatacgt ccaccgagga agacgccgag gaagcccgca tagagtaagt tttcaacttc
    37741 atttttggtc cagccactgc ccggacgatt cgcccgtcgt gttgccagcc acattgcaaa
    37801 aatgaaaccc accagataca tcaggccgta ccagtgaagc gccacgggtc ctattgagaa
    37861 aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc accacaagtt
    37921 cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt tcaggcgctc
    37981 caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg ttaaaagata
    38041 tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc gacgctccat
    38101 aaaggctgca acctgatggc gaacttcggt cgccagttgc gcttccagac tacgctgcgc
    38161 aagattttct gcgtcggcat aatcaatgcg ccgcagcagg tattttaccc gcgctacaga
    38221 acgtccgttc atagacagat gacgataccc aagcccaatg aggattgcca cgcacatggg
    38281 atcgcccgcc atttcaccgc acaaacggag atcgattcca tgtatttccg cttcccgggc
    38341 gatcatcgcc agagctcgta acattgcagg atgaagactg tcataaatgt tcgccacccg
    38401 ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc caacagagat
    38461 gaaatcgacc cgctttgcca gatgcggcag cataaatacc attgacggca cttccagcat
    38521 gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt cacgtccggc
    38581 acgttcaatc aggcggcgtg cttcatcaac ttcatcgagg cttgtgacca tcggcaacag
    38641 aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct ggatcaagaa
    38701 gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg gattctcttc
    38761 gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac gcaaggtgac
    38821 gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt cttcttccga
    38881 cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccga tgccatcaat
    38941 acggctgccc agtttttctt catgttccgg gcttaaacca gcattgagca tgacttttat
    39001 acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca gacggctaag
    39061 ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct ccggatcgac
    39121 cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca ccgaaggctg
    39181 aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg cagcatggga
    39241 gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca gctcagcaag
    39301 agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg cgttcggccc
    39361 ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta aatcgccagc
    39421 ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt tttcaatgac
    39481 cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca attcgcgacg
    39541 cagccgggtg tccgaaagta ggtgcgagta aagatcgaaa atagccgccg tttctttttg
    39601 tgctccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt cttccagcgc
    39661 cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat acacctgttc
    39721 cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag gtgctgccgg
    39781 taatgcgcgg attcgcgtct ggcgatattg cccaaacagg gcagtcaact gcgactgaga
    39841 aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac tttcgtcata
    39901 ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac gttgaataat
    39961 tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt atttgaagct
    40021 ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa ccaggccgac
    40081 gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca gcccccgggt
    40141 cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac agacctcggt
    40201 atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca ggcgtggtgc
    40261 gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg tgacttaacc
    40321 tcttttacgt cgataagcag atgcgttttg tggttttggc gtattttcct gcagtgacat
    40381 caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa atgacaccac
    40441 ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg gtgtactgct
    40501 ggtttgcata ttgatttctg catcgccgct caccagctgc aagagaaacc atttttgttt
    40561 ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta atttgtagcg
    40621 caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc ctacttcttc
    40681 aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc cgccttgcgg
    40741 aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct ggcgattaca
    40801 aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac tacctcaaaa
    40861 taaagcttta tatacgaatg attgtttcat actccaggaa gacggtaaac cactctctgc
    40921 agggcattac acactaataa caattgaata actcacagtt atgtgcagag ttataaacag
    40981 aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg tgaagaacta
    41041 cggaattact acgggaaaac ccggagcatt ct
//
LOCUS       DAPHJE010000031        40907 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPHJE010000031 DAPHJE010000000
VERSION     DAPHJE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.031"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            212..778
                     /gene="mntP"
                     /locus_tag="RH222_001641"
     CDS             212..778
                     /gene="mntP"
                     /locus_tag="RH222_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV3796683.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(775..1584)
                     /gene="rlmA"
                     /locus_tag="RH222_001642"
     CDS             complement(775..1584)
                     /gene="rlmA"
                     /locus_tag="RH222_001642"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV3796684.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1750..1959)
                     /gene="cspE"
                     /locus_tag="RH222_001643"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1750..1959)
                     /gene="cspE"
                     /locus_tag="RH222_001643"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV3796685.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(1972..2115)
                     /locus_tag="RH222_001644"
     CDS             complement(1972..2115)
                     /locus_tag="RH222_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV3796686.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2784..3071)
                     /locus_tag="RH222_001645"
     CDS             complement(2784..3071)
                     /locus_tag="RH222_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV3796687.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(3146..3289)
                     /gene="mgrB"
                     /locus_tag="RH222_001646"
     CDS             complement(3146..3289)
                     /gene="mgrB"
                     /locus_tag="RH222_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV3796688.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3448..3687
                     /locus_tag="RH222_001647"
     CDS             3448..3687
                     /locus_tag="RH222_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796689.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(3830..4621)
                     /gene="kdgR"
                     /locus_tag="RH222_001648"
     CDS             complement(3830..4621)
                     /gene="kdgR"
                     /locus_tag="RH222_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV3796690.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4798..6171
                     /locus_tag="RH222_001649"
     CDS             4798..6171
                     /locus_tag="RH222_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796691.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAAFVSGLRI
                     TQPRSRA"
     gene            complement(6217..7098)
                     /gene="htpX"
                     /locus_tag="RH222_001650"
     CDS             complement(6217..7098)
                     /gene="htpX"
                     /locus_tag="RH222_001650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV3796692.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7290..9338)
                     /gene="prc"
                     /locus_tag="RH222_001651"
     CDS             complement(7290..9338)
                     /gene="prc"
                     /locus_tag="RH222_001651"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV3796693.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9358..10056)
                     /gene="proQ"
                     /locus_tag="RH222_001652"
     CDS             complement(9358..10056)
                     /gene="proQ"
                     /locus_tag="RH222_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV3796694.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10153..10650)
                     /gene="msrC"
                     /locus_tag="RH222_001653"
     CDS             complement(10153..10650)
                     /gene="msrC"
                     /locus_tag="RH222_001653"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV3796695.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10780..12063
                     /gene="yebS"
                     /locus_tag="RH222_001654"
     CDS             10780..12063
                     /gene="yebS"
                     /locus_tag="RH222_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV3796696.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12032..14665
                     /gene="yebT"
                     /locus_tag="RH222_001655"
     CDS             12032..14665
                     /gene="yebT"
                     /locus_tag="RH222_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV3796697.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            14745..16184
                     /gene="rsmF"
                     /locus_tag="RH222_001656"
     CDS             14745..16184
                     /gene="rsmF"
                     /locus_tag="RH222_001656"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV3796698.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            16302..16538
                     /locus_tag="RH222_001657"
     CDS             16302..16538
                     /locus_tag="RH222_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV3796699.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16643..16834
                     /locus_tag="RH222_001658"
     CDS             16643..16834
                     /locus_tag="RH222_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV3796700.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(16835..17491)
                     /gene="pphA"
                     /locus_tag="RH222_001659"
     CDS             complement(16835..17491)
                     /gene="pphA"
                     /locus_tag="RH222_001659"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV3796701.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(17887..18228)
                     /locus_tag="RH222_001660"
     CDS             complement(17887..18228)
                     /locus_tag="RH222_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV3796702.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(18241..19113)
                     /gene="copD"
                     /locus_tag="RH222_001661"
     CDS             complement(18241..19113)
                     /gene="copD"
                     /locus_tag="RH222_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV3796703.1"
                     /translation="MLAFTWIALRFIHFTSLMLVYGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(19117..19491)
                     /gene="yobA"
                     /locus_tag="RH222_001662"
     CDS             complement(19117..19491)
                     /gene="yobA"
                     /locus_tag="RH222_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV3796704.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            19630..19860
                     /gene="holE"
                     /locus_tag="RH222_001663"
     CDS             19630..19860
                     /gene="holE"
                     /locus_tag="RH222_001663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV3796705.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            19962..20618
                     /locus_tag="RH222_001664"
     CDS             19962..20618
                     /locus_tag="RH222_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV3796706.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            20642..21304
                     /gene="exoX"
                     /locus_tag="RH222_001665"
     CDS             20642..21304
                     /gene="exoX"
                     /locus_tag="RH222_001665"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV3796707.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     YT"
     gene            complement(21301..23361)
                     /gene="ptrB"
                     /locus_tag="RH222_001666"
     CDS             complement(21301..23361)
                     /gene="ptrB"
                     /locus_tag="RH222_001666"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV3796708.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHYRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(23570..24229)
                     /locus_tag="RH222_001667"
     CDS             complement(23570..24229)
                     /locus_tag="RH222_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV3796709.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(24556..24912)
                     /locus_tag="RH222_001668"
     CDS             complement(24556..24912)
                     /locus_tag="RH222_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV3796710.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(24979..25269)
                     /gene="yebG"
                     /locus_tag="RH222_001669"
     CDS             complement(24979..25269)
                     /gene="yebG"
                     /locus_tag="RH222_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV3796711.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            25403..26581
                     /gene="purT"
                     /locus_tag="RH222_001670"
     CDS             25403..26581
                     /gene="purT"
                     /locus_tag="RH222_001670"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV3796712.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(26637..27278)
                     /gene="kdgA"
                     /locus_tag="RH222_001671"
                     /gene_synonym="eda"
     CDS             complement(26637..27278)
                     /gene="kdgA"
                     /locus_tag="RH222_001671"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV3796713.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(27315..29126)
                     /gene="edd"
                     /locus_tag="RH222_001672"
     CDS             complement(27315..29126)
                     /gene="edd"
                     /locus_tag="RH222_001672"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV3796714.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(29361..30836)
                     /gene="zwf"
                     /locus_tag="RH222_001673"
     CDS             complement(29361..30836)
                     /gene="zwf"
                     /locus_tag="RH222_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV3796715.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            31174..32043
                     /gene="hexR"
                     /locus_tag="RH222_001674"
                     /gene_synonym="yebK"
     CDS             31174..32043
                     /gene="hexR"
                     /locus_tag="RH222_001674"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV3796716.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            32171..33613
                     /gene="pyk"
                     /locus_tag="RH222_001675"
     CDS             32171..33613
                     /gene="pyk"
                     /locus_tag="RH222_001675"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV3796717.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(33744..34715)
                     /gene="lpxM"
                     /locus_tag="RH222_001676"
                     /gene_synonym="msbB"
     CDS             complement(33744..34715)
                     /gene="lpxM"
                     /locus_tag="RH222_001676"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV3796718.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(34835..36157)
                     /gene="mepM"
                     /locus_tag="RH222_001677"
     CDS             complement(34835..36157)
                     /gene="mepM"
                     /locus_tag="RH222_001677"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV3796719.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(36173..37105)
                     /gene="znuA"
                     /locus_tag="RH222_001678"
     CDS             complement(36173..37105)
                     /gene="znuA"
                     /locus_tag="RH222_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV3796720.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            37184..37939
                     /gene="znuC"
                     /locus_tag="RH222_001679"
     CDS             37184..37939
                     /gene="znuC"
                     /locus_tag="RH222_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV3796721.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            37936..38721
                     /gene="znuB"
                     /locus_tag="RH222_001680"
     CDS             37936..38721
                     /gene="znuB"
                     /locus_tag="RH222_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV3796722.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(39062..40072)
                     /gene="ruvB"
                     /locus_tag="RH222_001681"
     CDS             complement(39062..40072)
                     /gene="ruvB"
                     /locus_tag="RH222_001681"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV3796723.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(40081..40692)
                     /gene="ruvA"
                     /locus_tag="RH222_001682"
     CDS             complement(40081..40692)
                     /gene="ruvA"
                     /locus_tag="RH222_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV3796724.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(40831..40896)
                     /gene="yobI"
                     /locus_tag="RH222_001683"
     CDS             complement(40831..40896)
                     /gene="yobI"
                     /locus_tag="RH222_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDV3796725.1"
                     /translation="MYIFITHFFTEYVILKYLLPK"
BASE COUNT         9877 a        10493 c        10047 g        10490 t
ORIGIN      
        1 gccgatttcc agattccgga aatgtacgtg tcaacatact cgttgcaaaa cgtggcacgt
       61 acggactgaa tactttcagt caggcgagac catatgcaca tcaatcgcta tgcctgcatg
      121 aacgcgaaaa ccatcgcggc ttgttgttca tgtatgcatg tttgctgggg gcgatgatgt
      181 gttttatgga taccccggtc aggacattgt catgaatatc actgctactg ttcttcttgc
      241 gtttggtatg tcgatggatg catttgctgc atcaatcggt aaaggtgcca ccctccataa
      301 accgaaattt tctgaagcat tgcgaaccgg ccttattttt ggtgccgtcg aaaccctgac
      361 gccgctgatc ggctggggaa tgggcatgtt agccagccgg tttgtccttg aatggaacca
      421 ctggattgcg tttgtgctgc tgatattcct cggcgggcga atgattattg agggttttcg
      481 tggcgcagat gatgaagatg aagagccgcg ccgtcgacac ggtttctggc tactggtaac
      541 caccgcgatt gccaccagcc tggatgccat ggctgtgggt gttggtcttg ctttcctgca
      601 ggtcaacatt atcgcgaccg cattggccat tggttgtgca accttgatta tgtcaacatt
      661 agggatgatg gttggtcgct ttatcggctc aattattggg aaaaaagcgg aaattctcgg
      721 cgggctggta ctgatcggca tcggcgtcca gatcctctgg acgcacttcc acggttaata
      781 agaacgctgc cagaggtgaa tattaaagtc cgtctggcag tcgaacactt cttttgctgc
      841 cagtgtttgc cagacttctg gcttcgcacg ccaggcaaac ggcgtcatct gcaataatgc
      901 gacggcttca tcaccgcgaa gacgcatcgg ataacacaac tccgcactct gctgtaatgt
      961 aaaaccttcc agttgttctg catgaggtgc atgaagatgt acttcattgt aaatcagccc
     1021 cttcagctcc atcaaatgtc gcggtcccgg cgtggcagta atgacccagc cgccgggctt
     1081 cactactcgt gctaattctt ctgctttaca cggcgcgtaa atacgtatta tggcgtccat
     1141 actggtatcg gaaaacggca aacggtggct ggaagcgaca caaaaagtga cctgcggata
     1201 gcgtttcgcc gccgctttta tcgctacctt cgaaacatcc agaccaaacg tggtgatttc
     1261 gggcaacgca tcggcaaatg cgtgtgtgta atacccttca ccacagccaa tatccagcac
     1321 cgccgtggcc ttatcatcaa gccgttccct cagttgggcg acaattgcat cacgcagcgg
     1381 ctgataatgt ccggcatcta agaatgcgcg gcgtgcttgc atcatttccg cgctgtcgcc
     1441 cggatcacga gaccgtttat gctgaacggg cagcagattg acataccctt ctttcgccat
     1501 atcaaactga tgtcgctggg gacagatata gctgtttttt tcacgcgaaa gaggctgatg
     1561 gcaaagtgga caggaaaaag acatgacaac tccggcagga tactaaaggc cgcaagtgta
     1621 acgcgaatca cgcggcgggc aaatatccga acgccaggag cgaagataaa gcatagtaaa
     1681 aagcctcgca ttcgacgagg ctttatagcg agattgaagc gtattcacac ttcagatcag
     1741 tggattcgat cagatagctg ttacgttaac agctgccgga cctttctggc cgtcctgaat
     1801 ttcgaactca acgttctgac cttcagccag agttttgaag ccattaccct ggatagcgga
     1861 gaagtgtacg aacacgtctt tgctgccatc agccggagta atgaagccaa aacctttaga
     1921 ctcgttgaac cacttaacct gacctttaat ctttgccatt tgaaaaattc cttagattgt
     1981 tttcttcgcc cgcaggcata acatagataa aactgacaca ttactgcatg aggcaccaat
     2041 ataaggctcg gcagagaagc ggtattcaac gtcaacgtgt ttactcagga cttctttact
     2101 gaaaatgcca cacataaaca gaactgtacc tcgtttaacc cgaaatctgt tatcacatac
     2161 aacgttaatt atggcaagcc atttttaaac atgtctcgat cagacgcaca aatctcgata
     2221 cgtcttccac tttttttgca cacttatgca acggaatacg cgccgatata tcattgtgct
     2281 taaccttgcc agttcaggca gatacttaaa ctggcgtagt ttctaacata gttcaataag
     2341 ttcagttgtt ccaggccagt gggagaagtt attacatagt gcgtgcaata tcacattttt
     2401 tgctatgcaa tgaataaaaa gttatatcac tttttctcat aaaacagtca gttaacggct
     2461 atcaattacc ctaaagagaa gtcaatcccc aaagggattg aaaatttaaa ataagaaaaa
     2521 ttgatgaatg agcaaaaaat caagagagaa acgtttcgct aataaataat accacgctta
     2581 atcacaaatc cgatgtaaac atcctaataa attaatgagg aaaatatctt attctgatat
     2641 cccgttacgc aaataaataa tatgataatt atttgaagtc agaccagagt tttaatttga
     2701 agcagttgca ccatggcagt gcgtataatc aggatgaatt gagtaaccct tcaacgacgg
     2761 ggttactcaa gattgaggaa ggattatcgt tcggcgacca gacgaataat atcttcgtct
     2821 tcaaccgatt tttgactctc aacggtcttt ttatctgctt tctcaggttc gtttgcttcg
     2881 cacaaacgac gtaacagtgc attttgacgt ttttgctgat ccagcaacgc ctcaagcagt
     2941 tcaatctgtt cgttagtccg agaactggca cgattgataa aaaaccacaa gatgagcccg
     3001 ataagaagaa ccaccaccga tacaaccaaa gacgcaatgt tcatcacgcc tgaatttaca
     3061 acttcgttca tttcaccacc tctgagtaga ggcgctattc taccactgct ggagaggaag
     3121 aaaatctagt gctgaaaaaa tgatatcacc acgggataaa ctggttaatg gcacaaattc
     3181 cgctgaaaaa ttgtacatcc tgatcgcaca tcatgttgaa tacctgcgcc caaagcagca
     3241 agcaagccaa caccacgaca accagaacga cccatcgaaa ctttttcact ccaatctccg
     3301 cttcacctac cttatgtcat taaactagca tgcgttagtt aaacagcgcc taactatgtc
     3361 gatattcgtg ctttttcgga atgagtagct tgcttcagcg catattgccg ctacgattaa
     3421 gcgaacataa aaagagaaga ggttgtaatg cgattcatca ttcgcacagt tatgctgata
     3481 gcactggtat ggataggatt attactcagc ggatatggcg ttttgattgg aagcaaagag
     3541 aatgccgcag gattaggttt gcaatgtacc tatctgactg ccagaggcac cagcacggta
     3601 caatacttgc acactaaaag tggtttcttg gggataacag attgcccact gttacgtaaa
     3661 agcaatatcg tggtcgataa tggttaaggg taataaataa ccagattctc actcatgagt
     3721 aaaatattct tatagcaagc gcgtttaata taacgcacct tcaactttat ttttctcatg
     3781 ctaaataaag aacctgtctg tttgatattc cgacaggttc ttactctcat cagaacggat
     3841 agtcgtgata acccatttgg gcagaaattt tgcgcgctgc ggtatgcaac attgcgacat
     3901 attcctgtaa acgctcttca gagaaacgca acgtcgggaa ggagatgctc aaaccggcaa
     3961 tgaccacgcc aaagcgatca aataccggta ccgcaatgca tcgcagccct tcttcctgct
     4021 cttcgttatc ttcgccatac ccctgctcgc gcacctggtc cagaacgggt aataacgctt
     4081 ctgtactggt gatggtccgc tcggtactgc gtttatactc cacgccctca agaatttgct
     4141 tcacttcatc gcgatcgcgc catgccagca gtaccttacc aatcgcggtg ctgtacagcg
     4201 gattacgacg cccaatccgt gaatacatgc gcaaattgta catagagtca attttgtgaa
     4261 tgtaaacaat actgtcttcg tccagtgcgc cgaggtggat agtttctttg gtcaggcggg
     4321 agatctcacg catctggata tctgcgctac gaattaaatc gacgttttgt aacgcgcgag
     4381 cgcccagttc aaacaatttc aaggtcaggg aatatttctc cgactcccct tcctgcgcca
     4441 cataacctaa ggttttcatg gtctgtaaaa agcgataaac ggtgcttttt gacatcatga
     4501 cgcgctgcga cagctcggtt atccctattt cgcgctcttc acccagcgcc tgcaaaatgc
     4561 caaaaacttt tagcacggaa gatacagaat caggctgttt atccagatct gcgttagcca
     4621 tttatcacct cattgcgagt gttttataaa aatcagaact gttttttatt ataatttcgc
     4681 accagggtgg tcgcaatcca tcttttgccg gttagttaca attctgcgac atccaccgtg
     4741 aataacagtg ctagaatcat acccctgttg attattcacc aaagatataa aattcctatg
     4801 ccaaaagttc aggccgacgg cctgccattg ccccagcgat acggtgcgat attaaccatt
     4861 gtgattggta tttcgatggc tgtccttgac ggcgcaatcg ccaacgtcgc cctgccaaca
     4921 atcgccacgg accttcatgc cacgccagcc agttccatct gggtagtgaa cgcctatcaa
     4981 atcgccattg tcatctccct gctctcgttt tcgtttctgg gcgatatgtt tggctatcga
     5041 cgtatttata aatgcggtct ggtcgttttt ctgttgtctt cactgttctg cgccctttct
     5101 gattcgctgc aaatgctcac ccttgcgcgt gtcatacaag gtttcggcgg tgcagcgttg
     5161 atgagcgtta ataccgcact tatccgcctg atctatccac aacgttttct ggggagaggg
     5221 atgggcataa actcgtttat tgttgccgtc tcttctgctg ccgggccgac aattgctgca
     5281 gcaatcctct ccatcgcatc ctggaaatgg ttatttttaa tcaacgtacc gttaggtatt
     5341 atcgccctgc ttctggcgat gcgttttctg ccacccaatg gttctcgcgc cagtaaaccc
     5401 cgtttcgacc tgcccagcgc cgtgatgaac gcgttaacct tcggcctgct tatcactgcg
     5461 ttgagtggtt tcgctcaggg gcaatcgctg acgttaattg ctgcggaact ggtggtaatg
     5521 gttgttgttg gtattttctt tattcgccgc cagctttctc ttcccgtacc gctgctaccg
     5581 gtggatttac tgcgtatccc gctgttttca ctttctattt gcacatctgt ttgctctttc
     5641 tgcgcacaaa tgctggcaat ggtttccctg cccttttacc tgcaaaccgt gctcgggcgt
     5701 agtgaagtcg aaacaggttt acttctgaca ccgtggccgt tagcaacgat ggtgatggct
     5761 ccgctggcag gctatttgat tgaacgcgta catgcaggat tgctgggtgc tttaggatta
     5821 ttcatcatgg ctgcggggct tttttccctg gttctgctgc ccgcgtcacc tgcggatatc
     5881 aatattatct ggccgatgat cttatgtggc gctggatttg gcttgttcca gtcacccaat
     5941 aaccacacca ttattacctc cgcgcctcgc gaacgtagcg gtggagccag tggcatgtta
     6001 ggaacggctc gtctactggg tcagagtagc ggcgcggcgc tggtggcgct gatgctaaat
     6061 cagtttggag ataatggtac gcacgtctcg ctgatggctg cggctattct ggcagtgatt
     6121 gctgcctttg tcagtggttt acgtatcact cagccacgat ccagggcata aaaaaagcgc
     6181 gtcgatcagg acgcgctttt tagtatttac ttcatattac ttcaggtatt cacccgtacg
     6241 cagagcttca atacgtttat ccagcggcgg gtgagtcatg aacaactcac tgagcgattt
     6301 cgacttaccg ttaatgcaga aagccatcat gctggttgct tcttgcggtt catagctggt
     6361 tttcaggcgc tgcagcgcgg caatcatttt ctcgcgacca accagttttg ccgaaccggc
     6421 atcagcatgg aattcacgat gacgcgagaa ccacatggtg ataatgctcg ccagaatacc
     6481 aaacaccagt tccagaaccg ttgcaaccgc aaagtagatc agcgggttgc cgttgctctc
     6541 ttcaccttca tcacgatttc cgcccataaa acccgcggca agctgcgcca ggatacggga
     6601 aataaagata acgaaggtgt tcaccacgcc ctgaataagc gtcatggtga ccatatcacc
     6661 attggcgatg tggctgattt cgtgagcaat taccgcctcg gcttcatccg ggctcatgtt
     6721 ctgcagcaaa ccggtgctga cagcaaccag agaggcatca cggcgcgcac cggttgcaaa
     6781 agcgttgatg tccggcgcat ggtagatagc cacttgcggc atagcgatcc ccgcctgacg
     6841 agcctgggtt gctacagtat tgaccagcca acgttccctt tcgttacgcg gttgctcgat
     6901 cacttccccg ccaacagatc gtaatgccat ccatttggac atcagaagcg aaacgaagga
     6961 accaccaaaa ccgaacagca aggccatgat catcagcccc tgaacgctgc tcgactgtat
     7021 ccctgtcagg ctcagtacca gcccgaaaac gaccattacg gccaggttcg ttagcaggaa
     7081 gagcgcgatt cgcatcataa ttttcttttt acctcagttt aacaaaacgc aatatgcgat
     7141 acccacatcg tatgggttac gcgactattt tcaagtctgg atagtgcgta agtcaccaga
     7201 aagacacaac tttacatttt gtagcatctg atttacggca tcttgccgct gttaaaaaat
     7261 caggcacaat ttcttgtgcc tgattgatat tacttgacgg gagcgggttg ttccgcgggt
     7321 ctggcttttt caagcttcgc cagatcgagt gcgatattca ccgtctcatc cagataagga
     7381 tccggctcct ggtaatcttt tggtagatca tccagcttct tcaactccgg tttaccttcg
     7441 cgtttaaagc gttcgttcaa acgcgccaga cgcgtcgcat catcttcatt attctctttc
     7501 tcacgcacag cgtaattcag agaaacgata ttgcgcttgt ccttcatagc gttgaagcgc
     7561 gcgatatcct tcatgatgtt ctggaactca ggatctttcg cgatacgcgc attatgttcc
     7621 ttcagtagct ccggttcaaa ggccgttaaa tctcctgatt tcacataagt cgcggcatca
     7681 atgctatccc acggcagcgc gttatcttcg aatttctcac ccgtttccgt ttcttcatta
     7741 cccgtcggca tgatgatgtc tggcgttacg cctttacgtt gcgtactgcc gccgttaacg
     7801 cgatagaatt tctggatcgt gtactgcaca gaacccagcg ctggccattc aggacgtaac
     7861 atctgatcgt aaatacggtt caatgaacgg tattgctgaa cggtgccttt accaaacgtc
     7921 ggttcaccaa caaccagcgc acgaccgtag tcctgcattg ccgcggcaaa gatttctgaa
     7981 gccgaagcac tgaagcggtc aaccagtacc accagcgggc ctttatagaa gacctgtccg
     8041 tcggtatcgc tgtcttcacg aaccttgccg ttgttatcac ggacctgaac aatcgggccg
     8101 gatggaatga acagacctga gagcgataca gcttcggtca acgccccacc gccattgcta
     8161 cgcaggtcga tgatcacact gctgacattc tgtttttcca gtttctgcag ttgtactttg
     8221 acatcgtctg tcaaacccac atagaagccc ggaatatcca gcacgccgac tttctcttta
     8281 ccgacggtct tcaccgacat tttaaccgcg cggtcttcga gacgaatacg ttcacgggtc
     8341 aatgttacag tacgggtctt ggtcccttta ccagcaggta aaatttccag acgaacttta
     8401 ctgcccttcg gccctttaat taaggcaacc acatcatcaa gacgccagcc aatcacgtca
     8461 accatcggct tgcctgtttg accaacaccg acaattttgt caccaacgct gatagcttta
     8521 ctcttcgctg ccggaccacc tgccaccatc gaattgataa cggtgtagtc atcatccatt
     8581 tgcagcactg cgccaatacc ttccagcgac aaactcattt cagtgttgaa ctgttcggta
     8641 ttacgcgggg aaagatagtt ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc
     8701 agcgagaaaa catcttcgct gttggtttgc gccagacgac gaatggcaaa tttgtagcgg
     8761 cgtgtcaggg tttcacgaat ttctttatcc gtttttcctg ccagcttcag gcttaactcg
     8821 tcgaatttga ctttactgtc ccacagcgcg ttcaactcag cctcgttttt cggccagggc
     8881 gctttgctgc ggtcaaggtt ataagtgtcg ttgccggtga aatccatcgg cttttccagt
     8941 accgacaaag cgtactggta acgctcaaaa cggcgctttt gcgccagatt gtagagatcg
     9001 tagaaaacgt cgagtttgcc tgaacgcagt tcatcgccta actcggtttt ctttttcgcg
     9061 aactgttcaa catcgcttgc cagcagcacg ttgtggctgt aatcgagcag attcaggtag
     9121 cggtcaaaga ttttggccga aaatgcctga tcgaggtcga actggcgata atgagaacgg
     9181 gtgaagcgcg acgttacgcg ctcacttacc gtcgcatgct gcgtctcttc ctttaatacc
     9241 ggaatttgat cagcacgcgt gatatcttct acagcgaagg tctggcctgc tattgcaagc
     9301 aggccagcta acgcggtaag cctaaaaaac atgttcatgc ctggcccggc ctccgtttca
     9361 gaacactagg tgttctgcgc gcacaatcaa agacataccc gaattcagct ggacgcggac
     9421 gccgtctttg gtgatttcta atacggtggc atccatcgcg ttttgacccg ctttcacctt
     9481 cagggcttgt ccgacagtca gagctgaaat gtcagaaact ggggtgtgct gttcttcgcg
     9541 aggtgctttt actgtttttg gcgctttctc taccggcttt tgcgcacgag gtttacgttc
     9601 agcgccttct ttgcggcgtg gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc
     9661 ttctttctca ccagcagctg cggcagcttc gcgttttttc gcttgctgtt cagcacgctg
     9721 tgcctgaaca cgcgctttcg cttcttcaag ctgcttgcga gcatgctcta catgttgctc
     9781 gtccagctca ccgcatgggt tgccgtcaag atcgacacgc gttgcgccgg gtttaacacc
     9841 gtaaagataa cgccagctcg aagtgtagag acgtaaagcg gatcgtaatt gcgttttgct
     9901 caggttcatt tccccggcaa cacgatcgac caaatcctga aaaataccga ttttcagcgg
     9961 acgcgcttca ccttccgcac tgaaacagtg gggaaaacgt tcggccagaa acgcgattac
    10021 ttctttactg ctattcaact taggttgatt ttccatgaaa tttcctgatt acaacggacg
    10081 tagccaacaa gcgcaggcat gaacaggcgt cattataatg acgctaccag taaatgctac
    10141 gttatccgtt gattatcctg cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag
    10201 cactttttca agctgtgcca caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg
    10261 accgaagacg gtactatcga tgtcaagaac accaataatc tgatttttca ccaccagcgg
    10321 cagaacaatt tcagaattac tcgccgcatc acaggcaata tgcccgtcaa acgcgtgaac
    10381 atcctcgata cgctgcactt gattgcgggc aaccgcagtg ccgcacaccc cgcgcccgac
    10441 gggtatccgg acacaggcaa ttttgccctg aaatggtcct agtaccagtg tatcgtcctc
    10501 aagcaaataa aaacctgccc agtttacgtc agtgagacgc tcgtataaca acgcactggt
    10561 gttcgcaagc gttgccagaa aactggtttc tcccgccatc agcgcgttaa agtcgcgatt
    10621 taaatccgcg taaaattctg ttttgttcat tatataatca cttggttgtc ttacctggaa
    10681 ctgccagcct attaaaataa gcattaaatg cgttaatgct caagatcatt ccaatcatgg
    10741 gttaatatta atgttaattc ttattacatt tggcacgtca tggctcttaa cacaccacaa
    10801 attacgccga caaaaaagat aacagtgagg gcaatcggcg aggaactgcc gcgtggtgat
    10861 taccaacgtt gcccgcaatg tgacatgctg tttagcctgc ccgagataaa ttctcatcag
    10921 agtgcctatt gtccgcgctg tcaggcaaaa attcgcgacg ggcgcgactg gtcgctaacg
    10981 cgcctggcgg caatggcttt caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg
    11041 ctgttgcata tctggctgtt aggtattcgt atcgacgcca acgtcatgca aggcatctgg
    11101 caaatgacca aacagggcga tacgataacg ggggcgatgg tctttttctg cgttattggt
    11161 gcccccctca ttctggtgtc ctccatagca tatttatggt ttggtaaccg actgggaatg
    11221 aatttacgtc cggtactgct gatgcttgag cgtcttaaag agtgggtaat gctggatatc
    11281 tacctggtcg gcattggcgt tgcttctata aaggtacagg attatgccca tatccaggct
    11341 ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa cgacggtgac gttgtcacat
    11401 cttaatgttg aggagctgtg ggagcgatat tatccgcagc gccccgctac gcgtagggac
    11461 gagaaacttc gtgtctgtct tgggtgccat tttaccggct atcctgatca gcgtggtcgc
    11521 tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg
    11581 gcggcgctgt tagcgtcaat cgttttgttg ttaccagcca acctattgcc tatttctatc
    11641 atttatctga acggaggacg gcaggaagat acaattcttt ccggaattat gtcgctggca
    11701 agtagcaaca ttgcggttgc gggaatcgtg tttatcgcca gtattctggt accgtttact
    11761 aaagtgatcg tcatgttcac tttactgttg agcattcatt ttaaatgcca gcaaggttta
    11821 cgcacacgca ttctgttact gcggatggtg acctggattg gacgctggtc gatgctcgac
    11881 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
    11941 atgggaccgg ctgcgtttta ttttggcgca gccgtaattt tgactattct tgctgtggaa
    12001 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
    12061 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc ctttcatcgc
    12121 gctaatgatt gccggttggc tgatttggga cagttatcag gaccggggta ataccgtcac
    12181 catcgacttt atgtcggcgg atggtattgt tccgggccgt acgcctgttc gttatcaggg
    12241 cgttgaagtc ggaacagtgc aggatatcag cctcagcgac gatcttcgta agattgaagt
    12301 caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc gaagagactc aattctggct
    12361 ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg gacgccctcg tcggtgggaa
    12421 ctatatcggc atgatgccgg gtaaaggtaa agagcaggat cactttgtcg cactcgatac
    12481 ccaaccgaag tatcggctgg ataacggcga tctgatgatc cacctgcaag ctcccgatct
    12541 cggttcgctg aacagcggtt cattggtcta tttccgcaag atcccggtgg gaaaagtcta
    12601 cgactatgcc atcaatccca acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg
    12661 ttttaccgat ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
    12721 cgtcagtgtc agtggcgcga aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc
    12781 gattgccttc gattcacctg aagagtcgaa acctgccgag gcggaagata cctttggtct
    12841 gtatgaagat ctggcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
    12901 ggccggattg accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
    12961 aactaaactg gatttaaatc ctggtggtaa agtcactggg gaaatgacag ttgatcccag
    13021 cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta cgtaatccga aattatccct
    13081 tagcgatgcc aatctcagcg ccctgctgac tggcaaaacc ttcgagttgg tacccggcga
    13141 tggcgagcca cgcaaagagt tcgttgttgt gccaggcgaa aaagcactgc tgcatgaacc
    13201 tgatgttctg acgctgaccc tgaccgcgcc ggaaagttac ggtattgatg ctggtcagcc
    13261 gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca ccagcaaagg
    13321 cgtcaccttt accgtcgcca tcgagcctca gcatcgagaa ctggtaaaag gcgatagcaa
    13381 atttgtcgtc aacagccgtg tcgacgtgaa ggtggggctg gatggcgttg agtttctcgg
    13441 tgccagcgcc tcagaatgga ttaacggcgg gatacgtatt ctgccgggcg ataaaggcga
    13501 gatgaaagcc agctatccac tgtatgccaa tctggaaaaa gcgctggaga acagccttag
    13561 cgatttaccc accacaaccg tgagtttgag tgcagagacg ctgccggatg tgcaggcagg
    13621 atcggtagtg ctctaccgta aatttgaagt tggtgaagtt attaccgtcc gtccgcgagc
    13681 taacgcgttt gatatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
    13741 tagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg gcctgaccgt
    13801 acaggcatcc ccgctctcca gagcattaaa gggagccatt agcttcgata acctcagcgg
    13861 tgccagcgcc agtcagcgta aaggcgacaa acgaattctg tatgcttccg aaacagcggc
    13921 ccgtgcggtt ggtgggcaga ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
    13981 gatgccaatt cgctatctcg gtattgatat cgggcaaatc cagacgctgg atctgattac
    14041 cgcgcgcaat gaagtacagg caaaggcggt gctctatccg gagtatgtcc agaccttcgc
    14101 ccgcggcggt acgcgcttct cggtggtcac accgcaaatt tcagccgcgg gcgttgagca
    14161 tctagatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcgccg
    14221 cgactttgaa ttacaagagg ccaccattac tgattcgcgt tacctggatg gcttaagcat
    14281 tattgttgaa gcgccggaag ccggttcgtt aggtattggt acgcctgtgc tgttccgtgg
    14341 tctggaagtc ggtacggtta caggaatgac gctggggaca ttgtcagatc gcgtgatgat
    14401 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
    14461 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
    14521 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
    14581 gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
    14641 atggggaact gcgcttccca aataatgccc actgctccgg cgtgcctgcg ccggagcgtt
    14701 tatgctaaac tgcgcgcctg tttttttgcc agtggtacat gctcgtggcc caacacaccg
    14761 tttatttccc ggacgccttt ctgacacaaa tgcgcgaagc gatgccttcg acgctctcat
    14821 ttgatgattt tcttgccgcc tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc
    14881 tgaaaatctc cgttgctgat ttcctgcaat taaccgctcc ttatggctgg acgcttacgc
    14941 caattccgtg gtgtgaagaa ggtttctgga ttgaacgcga caatgaagat gcattgccat
    15001 tgggtagtac cgccgagcat ttaagcggcc tgttttatat tcaggaagca agttcaatgt
    15061 tgcccgttgc cgccttgttt gctgacgata atgcaccaca gcgggtgatg gatgtcgcag
    15121 ctgcgccagg ctccaaaacg acgcaaattg ccgcgcggat gaataacgaa ggggcaatcc
    15181 ttgccaatga gttttccgcc agtcgggtaa aagtgttaca tgccaatatc agccgctgtg
    15241 gcatcagtaa tgttgcgctc acacattttg atggccgcgt gtttggtgcg gcagtgccag
    15301 aaatgttcga tgccattttg ctggacgctc cctgctctgg cgaaggcgtg gtgcgtaaag
    15361 atcccgatgc gctaaaaaac tggtcaccag aaagcaatca ggaaatcgca gctacacaac
    15421 gggagcttat cgacagcgcc tttcatgcat tacgtcctgg tggtacgctg gtttactcga
    15481 cctgtacctt aaaccaggaa gaaaacgaag ccgtttgcct gtggctgaaa gagacttacc
    15541 ccgacgcagt agagttttta ccacttggcg atctcttccc tggtgcaaac aaagcgctga
    15601 ccgaagaagg ctttttgcat gttttcccac aaatttacga ctgcgaaggc ttcttcgttg
    15661 ctcgtctgcg taaaactcag gcgattcccg ccttacccgc ccccaaatac aaagtcggta
    15721 attttccgtt cagcccggtg aaagatcgcg aagctggaca aattcgtcag gcgactgcag
    15781 gtgttggctt aaactgggat gaaaacctgc gcctctggca gcgtgacaaa gaactgtggt
    15841 tgttcccggt gggcattgaa gccctgatcg gtaaagtccg attttctcgg ttggggatta
    15901 aacttgccga gacgcacaac aaaggttatc gctggcagca tgaagcggtt attgctcttg
    15961 cctcacccga taatatgaat gcattcgagc tgacaccgca ggaagcggag gagtggtatc
    16021 gcgggcgcga tgtttacccg caagccgcgc cagtggcgga tgatgtattg gttactttcc
    16081 agcatcaacc gattggttta gccaaacgga ttggttcgcg attgaaaaac agctatccgc
    16141 gtgaactggt gcgcgatggg aaacttttta ccggtaacgc ctgacagctc acaaaaaaag
    16201 cgcacttttt gactggcaca ttcggctgcc tcaactaggc tgaaaaatga tgcgatcgga
    16261 ctggtcgtac cacaatcggc agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca
    16321 taggcgcttt tgaaatcgac gacggcgaat tacacggtga atcgccgggt gatcgaacgt
    16381 taaccattcc ttgtaaatct gaccccgatt tatgtatgca actcgatgcc tgggatgctg
    16441 aaaccagtat ccctgccctg ctaaatggcg aacactctgt cctttaccgt acccgttacg
    16501 atcaacaatc tgatgcctgg attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat
    16561 ggcgggttat ttgtcctggt tattcccccg ttgtaaaatc tctcctaaac ttaacggtac
    16621 ggcaccacac ttcggggatg aaatgttcgc gctggtactt tttgtttgct acctggatgg
    16681 cggttgtgaa gatattgttg tggatgtcta caacacggaa cagcagtgtc tttattctat
    16741 gagcgatcaa cggatccgcc atggcggttg ttttccgatt gaggatttta tagatggttt
    16801 ctggcgacca gcacaggagt atggtgattt ttaattattg caattgcaca agagtcagtt
    16861 cgcccccaaa gacagcaccg gtatcaatat aatgcaggtt gccaatatcc acgcgatgtc
    16921 gcaacggtgt atgaccaaac cagaaatgat cagcacctgt aattccctgc cctttttggc
    16981 gttcacctaa tcgcgagcgg ctccacaaga cctgatgcaa atcaacgtcc ttttgccatt
    17041 cataaacatc atctggataa tcggcatgag caataacatg tttgccggta cgactgtgta
    17101 cttcaagaat aaagggcaaa tgctgacatt tttccagcgc cgttttcgct tgtttctgtt
    17161 gattatctgc cagcgcaata aaccagtcgc cgccattcat caaccacaaa gacatctgct
    17221 gggatgccag cgcatccatc gccatctgtt catgattgcc tcttaccgca caaacccaat
    17281 gttgttccag taactgcaga caacgtaaac tttgcggccc acgatcgata acgtctccta
    17341 ctgagataag taaatctcgc cacggatcaa aacgacaatg ccataatttg cggcgcaact
    17401 gctcaagaca accgtgtata tcgccagaaa gccagatatg tcgccattga tgacccgcaa
    17461 ttctctgata aacgggcgca ggctgtttca tcaatatttt cctcccgcgc taaagatcac
    17521 ataatcttaa caagaatgtt aaaaaacgct ggactcagac agtagagtgt gtgttatggt
    17581 tgactataaa gtcagcgaag gaaatgcttc tggcttttaa cagataaaaa gagaccgaac
    17641 acgattcctg tattcggtcc agggaaatgg ctcttgggag agagccgtgc gctaaaagtt
    17701 ggcattaatg caggcttagt tgccttgccc tttaagaata gatgacgacg ccaggttttc
    17761 cagtttgcgt gcaaaatggt caataaaaag cgtggtggtc atcagctgaa atgttaaaaa
    17821 ccgcccgttc tggtgaaaga actgaggcgg tttttttatt ggaaatcaaa aggctatttt
    17881 aggtaattaa cagagttttt cagctcgttc tataaacggt gccagactca ttttttcgcc
    17941 gggattgtta ggatcatcaa tctgaatcac cgaaatgggt tgggctttag tcttcccact
    18001 ggcaacttcc ttttgtgcga tatcgtttaa aggatactgc acgagggtac ttgggttaat
    18061 gacatacaaa gcattacccg gtctgcaagt cagcatcacc tcttcgcgat taaacgccca
    18121 tttgtcttta cccacttcaa aacgactgac ggtaatcacc tgcggtgcag ccagcgccgc
    18181 tgcagaactg gtgagtaaca gaaacgtcag aatacttttt ttcatcattt tcgtaatcca
    18241 tcaaaaaggt tcgagagtcg caagcaggct gatgatcacc agcactaccg cacctatcgc
    18301 ccattcgagt ttagtcatcc actcaaacca gggtgccgca cgatcttcat cttgcctcat
    18361 gcgcggtaca agaacataac gattagccag cgcaatgacc accataatca tcaccaggat
    18421 ggctttgagc aataagagtt ggccccagta agtcgtaagc gtggggggaa atccggtaat
    18481 caacaatgca ttaagcacgc cgcttgccag tacgccgatc accgcaaaat gcccgcacca
    18541 ggaaaaacgc atcagcgcct gaatggcctg gtgtcgccaa cggcctttga tgagttgcat
    18601 acaccagagc accggcagta atcccccaaa ccacgccgcc gcacaaatca ggtgaatcgc
    18661 gtgattagtc tgatggattt tcgccgttac cccttcattc agtgtcgcat gcccgattcc
    18721 cgccagcaga ataaattgcg cggtagtgag cataaacagc aaacgtggca tattccgcgg
    18781 ttgcataagg gcgacgatca acgttacgag ggcgagaaca atttgccata accagacgcc
    18841 accaaactgc gtttgcaaaa cagcctgcca aatgtttggc gaaaatacat ctgcccatcc
    18901 cgttcccatc aacccgccct gaacggcgag cattgcagtg gcactgatca aactccagac
    18961 ggcagcgtgc tgttgtaaac gcagaaaacg cttcgccagc aagcgacgaa tcgttaaggg
    19021 ggccagccag gcaccgtaca ttgcgaagcc atagaccagc atcagcgagg tgaaatggat
    19081 aaatcgcagc gcgatccagg taaacgccag catgatttat ttcacgctaa aggtgtagtg
    19141 tcctttcgtt ttatgcccgt ccaccgaaac aacatgccag tcaacgatgt aggttccggg
    19201 tttcagcgaa tcggccagtg gaacaatcag ttgcttttgg tcctgctcat ttcgcttcgc
    19261 cggtaatgtt ttaatatttt cgttttttgg ccccgtgatt tttgcaccac tgaatcctgt
    19321 ttcaacacct tccgagaagt ttaaagtgat tgcctgcggt gcagctgtca cttgcgcgtt
    19381 tgccgcagga tactgatgcg ttaaatgcgc atgtgcccag acggaaggtg tcaccaacga
    19441 agtggtcagg attgccagcg cgtagcgaag ggagcgtgca gttgaagtca tattatctat
    19501 tcctttttgt aataactttt ttacagagca taaccttgtc taatgtctga gtcgaggatc
    19561 atcaattccg gcttgccatc ctggctcact cttagtaact tttgcccgcg aatgatgagg
    19621 agattaagaa tgctgaagaa tctggctaaa ctggatcaaa cagaaatgga taaagtgaat
    19681 gtcgatttgg cggcggccgg ggtggcattt aaagaacgct acaatatgcc ggtgatcgct
    19741 gaagcggttg aacgtgaaca gcctgaacat ttgcgcagct ggtttcgcga gcggcttatt
    19801 gcccaccgtt tggcttcggt caatctgtca cgtttacctt acgagcccaa acttaaataa
    19861 aacttataca gagttacact ttcttacata acgcctgcta aattatgagt attttctaaa
    19921 ccgcacccat aatttgcagt cattttgaaa aggaagtcat tatgtcgttc tggaaagttg
    19981 cagcagcgca atatgagcct cgtaaaacct cactcaccga acaggtagct catcatctgg
    20041 agtttgtgag ggccgccgcc aggcaacaat gtcagctgtt agtttttcca tcgctttctt
    20101 tactggggtg tgattattcg cgacgtgccc ttcctgcccc acccgatctc tcactgttag
    20161 acccgctttg ttatgccgca acgacctggc gaatgaccat cattgccggc cttcccgttg
    20221 aatataacga tcgctttatt cgtggaattg cggtgttcgc accgtggcga aaaacgcctg
    20281 ggatctacca tcagagtcat ggtgcatgtc tgggtcgtcg ttccagaacc atcacggtgg
    20341 tcgatgaaca accgcaaggt atggatatgg acccgacctg ttcactgttt actacagggc
    20401 aatgtctggg agaacccgac ctgctggcgt cggcccgtcg cttacaattt ttttcacatc
    20461 agtactcgat tgccgtgctg atggcaaatg cccgtggtaa cagtgcacta tgggatgaat
    20521 atggtcgtct catcgttcgc gccgatcgtg gttcattatt gttagtcggt cagcgttcat
    20581 cacagggttg gcaaggcgat atcattccat tacgctaggc tttttcggcc tggagcatgc
    20641 catgttgcgc attatcgata cagaaacctg cggtttgcag ggagggatcg ttgagattgc
    20701 ctctgttgat gtcattgacg gaaaaatcgt caaccccatg agccacctgg tgcgccccga
    20761 tcgtcctatt agtccacaag cgatggcgat tcatcgcatc accgaagcca tggtcgccga
    20821 taaaccgtgg attgaagatg tgatcccaca ctattacggt agtgaatggt atgtcgcgca
    20881 taacgccagc tttgaccgcc gcgtactgcc tgagatgccc ggtgagtgga tttgcactat
    20941 gaaactggcc cgtcgtttgt ggcctgggat caagtacagc aatatggcgt tatataaaac
    21001 acgcaagctc aatgtacaga cgccgccggg cctgcatcat caccgcgcgt tgtatgactg
    21061 ttatatcacc gccgcgttgc ttatcgatat tatgaacacc tccggctgga cggcagaaca
    21121 gatggccgat atcaccggac gtccgtcgtt gatgacgacc ttcacctttg gcaaataccg
    21181 tggcaaagcg gtttccgacg ttgccgaacg cgatccgggc tatctgcgct ggttatttaa
    21241 taacctggac agcatgagcc cggagctgcg tttaacactg aaacattatc tggaatatac
    21301 ttagtccgca ggcgtagcgg gtaatgttcc ctgcgccagc gcgaccagaa aagcatattc
    21361 catcgctacg ccttcgtacg atttaaagcg accagattta ccgccatggc ctgagtccat
    21421 gtcggtacag agcaataaaa gatggtcatc ggttttcagc tcgcgcaatt tagcgaccca
    21481 ttttgccggt tcccaatatt gcacctgaga atcgtgcaaa ccggtcgtta ccagtaaatg
    21541 cggataagcc tgtgcggtga cgttgtcata cgggctgtag cttttcatgt actcgtaata
    21601 ttgcggatcc tgcgggttac cccactcttc aaactcacca gtggtaagag gaattgattc
    21661 atcaagcatc gttgttacaa catcaacaaa cggtacctgg gcgataacgc cgtggaataa
    21721 ttccgggcgt tgattaattg caacgcccat caacatgccc cccgcactcc cgcccatcgc
    21781 ataacaaagc gaaggagagc catagcccag ttttaacaat gcatcgcagg catcaagata
    21841 atcattaaac gtatttttct tcttcagaaa ttttccgtct tcgtaccatt gttgccccag
    21901 ctcaccaccg ccgcgaacat ggacaatggc gtagacaaag ccacgatcta acaaactcaa
    21961 gcggctaaaa ctgaaatcgg catcaatact tgcgccgtaa gaaccatagc catacaccag
    22021 caacgggttg tgtcctttgc gataatgttt gcgatggtag accaacgaaa ccggaacttc
    22081 gacgccatca cgggcgacta tccacaggtg ttcactgcgg taattcgccg catcaaaacc
    22141 gggcacttct gtttgtttta atacacgacg ctcaccggta tccatatcca gttcaaacaa
    22201 agtgtctggt gtggtcatgg aagaataacc ataacgcaat cgcgcggttt caggttctgg
    22261 attgtaggca atccaggtca cataggctgg atcatcaaag gcaataccaa tgacttcccg
    22321 ggtcttgcgg ttaatttggc gcaaactggt taacccgcgc tgacgctctt caaccaccag
    22381 ccagtcggta aacagcgtaa acccttccag catgatgttt tcgcgtggcg gaattaactc
    22441 ttcccactgt tgctcatcac gcatacgggt acggtataag ccaaagtttt tgccgtggcg
    22501 gttggaacgt agataaaaac gatgctggta gtgatcaagg ctgtattcgt gatctttgcg
    22561 gcgcggcaga aaaacaaacg gctcggcatc ggccatttcc gcgtccagca ggcgaacttc
    22621 actggtggtg gcgctggcca aatgaatgac tacatagtgc ttcgaggtcg ttttatgcag
    22681 gctgacgtaa taggtatcgt ctttttcttc gtagatcagt ttatcttgcg atgctggcgt
    22741 accgatggcg tgacgccaga cctgataagg cagcagcgtc accggatgct tgcgaacata
    22801 gtagaaagtc caggagtcat ttgcccagac aaagctgggt tcaacgttat ccagcagttc
    22861 cgggtaccag ttaccagttt ccagattacg aaaacgaatg ccgtactggc gtcgggaaag
    22921 aaaatcttct gccagcgcca taatggtgtt atcgggcgta atcgccattc cgcccatcga
    22981 ataaaactca ctatgagctg cgcgcttatt ggcatcgagc aatgtttccc actcatccca
    23041 ctcttcactg aatgccgatt gacgctggta gatagcatat tcacagcctg gttcataaat
    23101 atgccgatag cggtagccat ttttgatgta gggcgcagaa acttctcgtt gcggaatgcg
    23161 gtcgatgatt tcctttaaga tgcgatcctg caaggcttgt tgtgaggcca tcacccgatg
    23221 accgtaacta ttttcttgtt gcaggtagtc caggacttct ggctgagaac gcgtatcgtc
    23281 ccgcagccag tagtaattat cgatgcgcgt atcgccatga agcgtcatgg cgtggggaat
    23341 gcgggcggct tttggtagca tgttattgtt ctttctggtt gaaacatctt ataagggtgg
    23401 caaaactcac cggggatgca agcgaaacag gggagtcatt gcttagatga tgacaggtaa
    23461 tggcgcggat atcgaatgtt atgcagacag agaaaatcag cctgttcaaa tggctgtgcg
    23521 attctggata gcccgaaata gtcaacttca ggctatccag agagcggaat tattccgcca
    23581 aagtgcgttt ttgctgttcg agatcgcgtt caatgccgtc acgaacatcc tgggggattt
    23641 tcagcgcgtc acccagtgca ttcaggtaac tgcgttccat aaaatggtca atatcaatag
    23701 ccgcgcaact caggaaatag atttccagcg cctcttcttc attgcggacc ccggtagcca
    23761 ggcgttgtgg atccagcggt tgttcgattg cctgctcaat gagtacacgc ccctgctctt
    23821 ccacgccggc ttcacgcaat tgctggtcga ttgccgcacg ttctttggca tcaatatgac
    23881 catcactttt agcggcaaag accagcgcaa ggatcaaacg ttctgtacgc tcatccagcg
    23941 gcgtactttg cgcgccaaac tgcggttcgt cctgatgcgc cgcgcgaatt ttatctttgt
    24001 atttattcca cagcaccgta cccgctaccg ctccgccgcc aaccagtaac gcgttggtgc
    24061 catatttcgt aagaagttta cgtgctgatt tatttgcgac cagcagccca gccagtccgc
    24121 ctaatgcgcc tggcactaac agtttgccca atccctgatc cgcagacgag gaggtagaag
    24181 aactgctttg cccaagcagg gattgcagtt gatttaacca gttagccata tttgctcctc
    24241 aataaccatt tttagtgcct gtagcatagc ggatgaggat gtcagaaaat gtatatcgag
    24301 acaaaagatg cgcaaataag cggggcggta ggccgttatt caaagaaatt cgcgccaggt
    24361 aaggtattta ctgtatatca ggtgataagg gtattttagg tgaaagtagt ttgtcgtttt
    24421 gagtggcatt gtttgatgct gattgcccga tgcgacgctg ttgcgtcttc tcaggcctac
    24481 gattgtggtg cgttctgggg cggacaaggc actcgcgccg catccgccaa cggtcaatcg
    24541 aattatcaga agccattaac gccgctgata ttccgccatt cccgctttgc agtccacgct
    24601 gacctggtaa tgaatatcgg cacttttacc acgcacggtt agcggtactg accatttatc
    24661 atctttaccc tgaatgtcct gcaaactgac ccaagccacg ggatcggcct gaccgacaat
    24721 tttttgatca tctgcccagc gcgccacgcg attttgttga taatcacgtt ttacgctcgc
    24781 ggcaattccg gcagcatcca gaccttcaca ctttgggaaa gtgaccgact tgctggtttc
    24841 attattggca gcgaaaactg atgcgcaggc agaaaccaac aacagcccta aaaacgcccc
    24901 tctttttttc atgtttttct ccatagcaca atgattcagg agaaagcatg gtacaaattg
    24961 tcaggagcgc aagttgcttc aggccgcgtg tgaggcatct tcctcttcgg attcagcacc
    25021 gacgacctgt ggggacggta atttgccggt tttcagaatg gtgctcagca catctttttg
    25081 ttctgccagc catagcgaaa gggcttcacg ttgctcgtct tccatttgca ctggagaatt
    25141 tgtcagccag gtgtcaagca gatccgccgt atcaagcatt ttatcataag catcggcttc
    25201 ctttttgctg gtaaacgaca ttttctcttc gccctcacga atgactacgt atttaacttc
    25261 aaccgccatt tgcagcctct cataataact gtgattttat acagtatatt tcttttcggt
    25321 tgagaaatca acatcagcaa taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg
    25381 aattaatcag gggatattcg ttatgacgtt attaggcact gcgctgcgtc cggcagcaac
    25441 tcgcgtgatg ttattaggct ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg
    25501 tctcggcgta gaggtgattg ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc
    25561 gcatcgctcc catgtcatta atatgcttga tggtgatgca ttacgccgtg tggttgaact
    25621 ggaaaaacca cattatatcg tgccggagat cgaagctatt gccaccgata tgctgatcca
    25681 acttgaagag gaaggactga atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa
    25741 tcgcgagggt atccgtcgcc tggcggcaga agagctgcag ctgcccactt ccacttatcg
    25801 ttttgccgat agcgaaagcc ttttccgcga ggcggttgcc gccattggct atccctgcat
    25861 tgtaaaaccg gtgatgagct cttccggcaa ggggcagacg tttattcgtt ctgcagagca
    25921 acttgctcag gcatgggaat acgctcagca aggcggtcgc gccggagcgg gccgcgtaat
    25981 tgttgaaggc gtcgttaagt ttgacttcga aattaccctg ctaaccgtca gcgcggtgga
    26041 tggcgtccat ttctgtgcac cagtaggtca tcgccaggaa gatggcgact accgtgaatc
    26101 ctggcaacca cagcaaatga gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa
    26161 agtggtgctg gcactgggcg gttatggtct gtttggtgtc gagctgtttg tctgtggtga
    26221 tgaggtgatt ttcagtgagg tctcccctcg tccacatgat accgggatgg tgacgttaat
    26281 ttctcaagat ctctcagagt ttgccctgca tgtacgtgcc ttcctcggac ttcctgttgg
    26341 cgggatccgt cagtatggtc ctgcagcttc tgccgttatt ctgccacaac tgaccagtca
    26401 gaatgtcacg tttgataatg tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt
    26461 tggtaagccg gaaattgatg gcagccgtcg tctgggtgtt gcgctggcga ccgctgagag
    26521 tgttgttgac gccattaaac gcgcgaagca cgccgccgga caggtaaaag tacagggtta
    26581 aacccggcaa aaaaacgcta caaaaatgcc cgatccgcga tcgggcattt tgacttttac
    26641 agcttagcgc cttctacagc ttcacgcgcc agcttagtaa tacggtcgta atcgcccgct
    26701 tccagcgcat ctgccggaac cagccaggaa ccaccgatgc acagcacgct tttcagcgcc
    26761 aggtagtcac ggtagttagc cggagaaata ccacctgtcg ggcagaaacg gacctgggag
    26821 aacggacccg cgatcgcctg cagggctttc acgccgccgt tagcttcagc tgggaagaat
    26881 ttgaactctt tcaaaccgta gtccataccc agcatcagtt cggaaacagt gctgatcccc
    26941 ggaatcagag gaatagtccc ttcggtagca gctttcagca gcggctcggt cagacccggg
    27001 ctaattgcga actgtgcacc cgcttcggtg acttctgcca gctgctgtgg attcagcacc
    27061 gtaccggcac ccacaatcgc ttcaggcact tctttggcga tagcacggat agcgtcaact
    27121 gcacactcgg tacgcagagt cacttccaga acgcgcaccc caccagcaac caacgctttt
    27181 gccatcggca ctgcgtgttc cagttttttt accacgataa ccggtacaac cgggccggtg
    27241 gtcaggattg attctgcact tgttttccag tttttcatca gagttttctc tcgcctgatt
    27301 acaaatttgt cgtcttaaaa agtgatacag gttgcgccct gttcggcacc ggacagtttt
    27361 tcacgcaagg cgctgaataa ttcacgtcct gtccccacgc gtgacgcgct caggtcagga
    27421 atgtgcggtt cgcgagcagc cagttccgct tcgtctacca gcagcgtcag ttcgcctgtc
    27481 tgtccattca cacgaatgat gtccccgtcg cgcacttttg ccagcagccc gccatcgtag
    27541 gcttctggtg ttacgtggat agctgacggc actttacctg aagcgccgga gagtcgtcca
    27601 tcggtaacta acgcaatttt gaaacaccgg tccaataata caccaagtgg cggcatgagt
    27661 ttatgtaatt ctggcattcc gttcgctttt ggcccctgat gacggacaac aacgacacaa
    27721 tcgcggtcca gcaaacccgc ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca
    27781 accgctggcg cttcaatcac ctggttctca accggcacgg cagaggtttt cataaccgca
    27841 cggcccaggt taccgcttaa cacttttgtc ccaccatgat gagagaaagg ttgttcgaag
    27901 gaagcgatca cattgctgtc gagtgatttt tccgcccctt cccgccagtc cagttcacca
    27961 ttattcagcc atggttcaag ggtataacga gacagaccaa agcctgccac cgtattgaca
    28021 tcttcatgca gcaggcctgc tttgagcagt tcacgcacca gaaccggtac gccacctgcc
    28081 gcctggaagt ggttaatatc ggccggaccg ttcgggtaga gacgtgccat cagcggtaca
    28141 acatcagaaa ggtcagagaa gtcatcccag ttaatctgaa taccggccgc gcgcgccatc
    28201 gccaccaggt gcatggtgtg gttagtggaa ccaccggtcg ccagcagtgc aacgataccg
    28261 ttcaccacca ctttctcatc gatcatctta ccgatcggca tccattcatt accattaccg
    28321 gtcatgcgtg taacctgacg cgcagcggca gcggtcaaag catcgcgcag cggagaatcc
    28381 ggatgaacaa aagaagagcc tggcaactgc atccccataa attccaccac catctggttg
    28441 gtgttggcag taccgtagaa agtacatgtt cccggcgcat ggtaagacgc ggcttctgac
    28501 tccagtaagg ccatgcggtc cactttacct tcggcataaa gctggcgaat acgcactttt
    28561 tctttatttg gcaaaccact tgccatcggt ccagacggca caaacaccgc aggcaaatga
    28621 ccaaacgaca gggctgccat cgtcagaccc gggacaatct tgtcgcacac accgaggaac
    28681 agagcaccat caaacatgtt atgggacagc ccgaccgccg cagacatcgc tatcacttcg
    28741 cggcttagca gcgacaattc cattccatcc tgcccctggg tgacaccatc acacatcgcc
    28801 ggaacaccgc ccgcaacctg accaaccgca ttcgcttcat gcagggcttt acgaatgatt
    28861 tctggatagt gttcataagg ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg
    28921 gcgatattgt tacgcaacat gcttttcaaa gaggctttgt cttctggctg gcaggcagcg
    28981 aaaccgtgtg ccaggttacc gcatgccaac tgcgaacgat gaacagtcga cgttttcgct
    29041 tgttctatcc gggcgagata agcagagcga gtctcgcgcg aacgttcaat gattcgattt
    29101 gttacgcgta acaattgtgg attcataaag gctcctgaaa ttgagttgtc agagcaggat
    29161 gattcacaac gcgtttcatt cagaggattt atgactgaaa cgcctgtaac cggagctcat
    29221 agggcaaaaa cgtttcagtc agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg
    29281 cctgaaagtg taaaaattgt tctacaatct gtgcaagatc atgttaccgg taaaataacc
    29341 ataaaggata aacgcagata ttactcaaac tcattccagg aacgaccatc acgggtaatc
    29401 atcgccaccg aggcaacggg tccccaggtt ccggcctgat acggtttcgg cgcatcattg
    29461 tccatcgccc acgcctcagt aatggagtct acccatttcc aggcttcttc cacttcgtcg
    29521 cgacgtacaa acagtgcctg aataccacgc atggtttcca gcagcaaacg ttcataggca
    29581 tccgccagat gcgtctgatt aaaggtttct gaatagctca gatccagctt ggtgatttgc
    29641 aggttatgtt tgtggtcaag gccaggaact ttattcagta cctggatatc cacgccttca
    29701 tcaggttgca gacggatagt cagtttattc tgcggcagat cctgccacga ctctttaaac
    29761 agattcagtt caggtgtttt gaaatagacc acgacttcag aacatttggt cggcagacgt
    29821 ttaccagtac gcaggtagaa tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg
    29881 atcgccacga aggtttctgt attgctgctc ttgttcgcgc cctcttcttc cagatatccc
    29941 ggcacttttt tgccctgggc gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt
    30001 acgttggagc ggtcgatgcg gcgcagagac ttcagtactt tcactttttc atcgcggatg
    30061 ctgtctgcgc tcaggtcaga cggcggagac atcgcaatca tgcaaagaat ttgcagcagg
    30121 tggttctgga tcatgtcgcg catctgaccg gctttatcaa aatagcccca gcgcccttcg
    30181 atccccactt cttctgccac ggtaatctca acatgatcaa tggtgcgatt gtcccagtta
    30241 ttcacaaaca gggagttagc aaaacgcagc gccaacaggt tcagcaccgt ttctttacca
    30301 agatagtggt cgatacggta aacctggcac tcctcgaagt attcgccaac ctgatcattg
    30361 atttcctgcg aggtcgccag cgacgtcccc agcggtttct ccatgactac gcgtgccggt
    30421 ttagcattca gtttagcctc gccaagccct ttgcaaattg cgccaaaagt gctgggcggc
    30481 atggcaaagt agttaatggt gatacgattt ttttgatcca gcatcgcgcc gagacggctg
    30541 aatgcagcag tgtcattgac atcgagatta caaaaatcca gacgtgcact cagggtgtcc
    30601 cataaacctt catcaatggt ttctttcatg aaagtttcga gcgcctcgcg gacaactttg
    30661 gtatatgccg ctttatccca gtcagcacgc cctacgccga taatccgggt gtccgggttg
    30721 agctgaccgg ctttttccag ttgatacagg gaaggcagca atttacgacg cgcaaggtcg
    30781 cctttcgcgc cgaaaatgac caggtcacag gcctgggctg tttgcgttac cgccatgtca
    30841 ttctccttaa gttaactaac ccggtactta agccagggta tacttgtaat tttcttacgg
    30901 tgcactgtac tgcttttacg agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt
    30961 acgggaaaag cgcgcaaagt gcggcaaaac tgataaaaaa atcatcgttt tccattgggt
    31021 aaaaatctga cactgatcat gttatgaaaa aaaataacaa cttttttatc tgcttttgtc
    31081 attaacacgg cacacaggcg taatatctga caaaactgca tttcgatttc tttcagtgcg
    31141 gaaatcgtca ttacccgtga gtctctttac atcatgaata tgctggaaaa aatccagtct
    31201 cagctggaac atttgagcaa atcagagcgc aaagttgccg aggtcattct ggcttcgccc
    31261 gataacgcga tccattcgag tattgctgct atggcactgg aagccaatgt tagcgaaccg
    31321 acggtgaatc gtttctgtcg cagcatggac acgcgcggtt ttcctgattt taaacttcat
    31381 ctggcacaga gtctggcgaa tggcactccc tatgttaatc gcaatgtcaa tgaagatgac
    31441 agcgttgaat catacacagg gaaaatattt gagtccgcaa tggcaacgct tgatcatgtc
    31501 cgtcattcac tggataaatc tgccatcaac cgcgccgtcg acttgctcac tcaggcaaag
    31561 aaaatcgcct ttttcggatt aggctcttca gccgccgttg cccacgatgc gatgaataag
    31621 ttctttcgtt ttaatgttcc ggtggtgtac tccgatgata tcgtgctgca acgcatgagc
    31681 tgtatgaatt gtagcgacgg agacgtggtg gtgctgattt ctcacactgg aagaacaaaa
    31741 aatctggtcg agctggcgca gctggcacgc gaaaacgacg ccatggtgat tgccctcacc
    31801 tctgcgggta ccccgctcgc ccgggaagca acgctggcaa ttaccctcga cgtaccggaa
    31861 gatactgaca tttatatgcc catggtttct cgacttgcac agctgaccgt gatagatgtg
    31921 ctggcgacag gatttacttt gcgacgcggt gcaaaattca gagataactt gaagcgggtc
    31981 aaggaagcgc tgaaggaatc gcgttttgat aagcagttac ttaatttaag tgacgatcgc
    32041 taaaaacgac tgtcactgtc ctaatcttat acgacatccg aatgagatta atttatcgcc
    32101 atcgcggcgt tatttcattc ggatttcatg ttcaagcaac acctggttgt ttcagtcaac
    32161 ggagtattac atgtccagaa ggcttcgcag aacaaaaatc gttaccacgt taggcccagc
    32221 aacagatcgc gataataatc ttgaaaaagt tatcgcggcg ggtgccaacg ttgtacgtat
    32281 gaacttttct cacggctcgc ctgaagatca caaaatgcgc gcggataaag ttcgtgagat
    32341 tgccgcaaaa ctggggcgtc atgtggctat tctgggtgac ctccaggggc ccaaaatccg
    32401 tgtatccacc tttaaagaag gcaaagtttt cctcaatatt ggggataaat tcctgctcga
    32461 cgccaacctg ggtaaaggtg aaggcgacaa agaaaaagtc ggtatcgact acaaaggcct
    32521 gcctgctgac gtcgtgcctg gtgacatcct gctgctggac gatggtcgcg tccagttaaa
    32581 agtactggaa gttcagggca tgaaagtgtt caccgaagtc accgtcggtg gtcccctctc
    32641 caacaataaa ggtatcaaca aacttggcgg cggtttgtcg gctgaagcgc tgaccgaaaa
    32701 agacaaagca gacattaaga ctgcggcgtt gattggcgta gattacctgg ctgtctcctt
    32761 cccacgctgt ggcgaagatc tgaactatgc ccgtcgcctg gcacgcgatg caggatgtga
    32821 tgcgaaaatt gttgccaagg ttgaacgtgc ggaagccgtt tgcagccagg atgcaatgga
    32881 tgacatcatc ctcgcctctg acgtggtaat ggttgcacgt ggcgacctcg gtgtggaaat
    32941 tggcgacccg gaactggtcg gcattcagaa agcgttgatc cgtcgtgcgc gtcagctaaa
    33001 ccgagcggta atcacggcga cccagatgat ggagtcaatg attactaacc cgatgccgac
    33061 gcgtgcagaa gtcatggacg tagcaaacgc cgttctggat ggtactgacg ctgtgatgct
    33121 gtctgcagaa actgccgctg ggcagtatcc gtcagaaacc gttgcagcca tggcgcgcgt
    33181 ttgcctgggt gcggaaaaaa tcccgagcat caacgtttct aaacaccgtc tggacgttca
    33241 gttcgacaat gtggaagaag ctattgccat gtcagcaatg tacgcagcta accacctgaa
    33301 aggcgttacg gcgatcatca ccatgaccga atcgggtcgt accgcgctga tgacctcccg
    33361 tatcagctct ggtctgccaa ttttcgccat gtcgcgccat gaacgtacgc tgaacctgac
    33421 tgctctctat cgtggcgtta cgccggtgca ctttgatagc gctaatgacg gcgtagcagc
    33481 tgccagcgaa gcggttaatc tgctgcgcga taaaggttac ttgatgtctg gtgacctggt
    33541 gattgtcacc cagggcgacg tgatgagtac cgtgggttct actaatacca cgcgtatttt
    33601 aacggtagag taagtacgtt gccggatgcg gcgaaaacgc cacatccggc ctacagttca
    33661 atgatagttc aacagatttc gaatattctg aagcaaactt gaacttatca tcaggcgaag
    33721 gcctctcctc gcgagaggct tttttatttg atgggataaa gatctttgcg cttatacggc
    33781 tggatttcgc ccggtttgcg agttttcagc aattttagta tccaggtgta ttgttctggt
    33841 cgcggaccaa caaaaatctc gacttcttca ttcatccgcc gcgcaatcgt atgatcatcc
    33901 gcctctaaca gatcatccat cggtgggcgc acctgaatcg tcagacgatg cgtcttgcca
    33961 tcataaatcg gaaacagcgg tacaacgcgc gcacggcaca ctttcatcaa acgaccaatc
    34021 gcgggcaacg tcgctttata ggtggcaaag aaatccacaa attcgctgtg ctctgggcca
    34081 tgatcctgat cgggtaaata atatccccag tacccctgac gtaccgactg gatgaatggt
    34141 ttaataccgt catttctcgc atgcagacga ccgccaaagc gacgacgcac cgtgttccag
    34201 acataatcaa aaaccgggtt gccctgatta tggaacatcg ctgccatttt ctgcccttgc
    34261 gaggccatca gcatggcagg aatatcgacg gcccaaccgt gcggcaccag aaagataact
    34321 ttctcgttat tacgccgcat ctcttcgatg atctccagcc cttgccagtc aacgcgcggc
    34381 tgaattttct ccggcccgcg tattgccaac tcagccatca ttgccatcgc ttgcggcgcg
    34441 gtggcaaaca tctcatcaac aatcgcttcg cgttcagctt cactacgttc tggaaagcag
    34501 agcgacagat tgattaacgc acgacggcgt gagctttttc ccagtcgtcc ggcaaaacgt
    34561 cccagccgtg ccagaatggg atcacggaac tttggcggcg ttaaagcgat acccgccatc
    34621 gctgctacgc ccagccatgc tccccagtag cgcgggtggc gaaaggattt atcaaactca
    34681 ggaatgtatt cgctattatt ttttttcgtt tccatgcttt tccagtttcg gataaggcaa
    34741 aaatcaatct ggtgatagtg tagcggcgca acttgccccg caccaaataa aaaaagccgg
    34801 tactgactgc gtaccggctg cgaatggatg ttaattaatc aaaccgtagc tgcggcacaa
    34861 tctctttggc ctgcgccagg aattcgcgac gatcggagcc ggtcagccct tcggtacgcg
    34921 gcagttttgc cgtcagcggg tttacggcct gctggtttat ccatacttca tagtgcagat
    34981 gcggcccggt tgaacgtccg gtattaccgg aaagcgcgat acggtcgcca cgtttcacct
    35041 tctgtcccgg tttcaccaga atcttgcgca agtgcatata acgcgtggtg tagctgcgac
    35101 catgacgaat agccacataa taacctgctg cgccactgcg tttggcaacc accacttcac
    35161 cgtcacccac tgaaagcact ggcgtaccct gcggcatggc gaaatcaaca cctctgtgtg
    35221 gtgcaacgcg accggtcacc ggattagtac gacgcgggtt aaagttagac gagatacgga
    35281 actgtttcgc cgtcgggaat cgcaagaatc ctttcgccag accagtaccg ttacgatcgt
    35341 agaatttgcc atcttcagcg cggatagcgt aataatcttt accttctgaa cgcaaacgta
    35401 cgcccagcag ctggctttgc tcacgtttac catcaagcat ttctcgagac attaacaccg
    35461 caaattcatc gccttttttc agtttgcgga aatccatttg ccactgcatg gctttaatca
    35521 ctgcgctcac ttcggcgctg gttaaaccgg cgtttctggc gctggcaaca aagcttcccc
    35581 cgacggtacc tttcagcaga ttgttaaccc actctccttg ctgcatttcg ctggtcattt
    35641 taaaaccgtt agcggcagta cggtcatagg ttcgggtttc acgacgagac acttcccagg
    35701 tgaggcgctg cagttcgccg tccgcggtta atgtccagga gagttgttga ccgattttca
    35761 ggttacgcaa ttctttgtcg gccgcagcca gttgggtgat atcacccata tcaataccat
    35821 actgattgag aatgctgctt agcgtatcgc cagtggaaac aacatattca tgcacgcccg
    35881 cttcaccggc gattttgtca tccagttcgt cctggggaat ggcttcatct tcttgtgcag
    35941 cttgatcaat cggctcactg gcttcaggta agagcgaacg aatttcgttc tgttccagct
    36001 caatggtttt gacaattggc gtggcatcac ggtgataaac ataaggccgc cagacagcga
    36061 cggccagagt aagaacggtg agcgacccca acataacgcg gtgtggtcgc ggtaaattat
    36121 taaacgccag ggcgacagag cgggctatct gttgcacgta atcacttcct cattaatctc
    36181 ctttcaggca gctcgcatac tggttggcta attgactcag gaattctgaa tagcttgttt
    36241 tacccagttt gatattcgtc cccaggggat ccaacgttcc catacgaacg gatgtccctc
    36301 gtgcgacgct ctcaacgacc gctggcctga actgtggctc agcaaaaacg caggttgctt
    36361 tttgctcaac caactgtgtt cttatttcat gtaaacgctg cgcgccaggt tgaatctcag
    36421 ggttaacggt aaaatgacca agcggtgtca gtccaaactg tttttcgaaa tagccgtaag
    36481 catcgtgaaa aacgaaataa cctttcccct tgagcggcgc gagctcgtta ccaacctgcg
    36541 tttcggttga ggctaattgt gcctcaaaat ccttcaggtt ggcgtcaagt ttggctcgac
    36601 tttgcggcat aagttccact aattttccat ggattgcaac cgctgtagcc cgcgctatct
    36661 ctggggaaag ccaaagatgc atgttgaaat cgccgtgatg gtgatcttcg tcactttttt
    36721 ccgcgtggtc gtgatcatca tcatcgccgt gaatactttt catcagtagc ggtttcacat
    36781 cttcaagctg cgcaatcgtt acctgcttcg cttctggtaa tttgcttacc ggtttttgca
    36841 taaacgcttc catctccggg ccaacccaaa cgactaagtc cgcgttctgt aagcgtttta
    36901 catccgatgg acgcagtgaa taatcatgtt cagaagcgcc gtcaggcagt aaaacctccg
    36961 tttctgttac accatcagca atggcagaag cgatgaaccc aacgggttta agcgaagcga
    37021 caacggcggc atctgcggcc tgtgttgcac ctccccagag agcggcggat aatgctgcga
    37081 aaagaagcgt ttttttatgt aacataatgc gaccaatcat cgtaatgaat atgagaagtg
    37141 tgatattata acatttcatg actactgcaa gactaaaatt aacatgacaa gtctggtttc
    37201 cctggaaaat gtctcggttt cttttggcca acgccgcgtc ctctctgatg tgtcgctgga
    37261 acttaaacct ggaaaaattt tgactttact tgggccaaat ggcgcaggta agtcgacact
    37321 ggtacgggta gtgctcgggc tggtaacacc cgatgaaggg gttatcaagc gcaacggaaa
    37381 actacgcatc ggctatgtac cgcagaagct gtatctcgac accacgttgc cactgaccgt
    37441 aaaccgtttt ttacgcttac gccctggcac acataaagaa gatattttgc ctgcactgaa
    37501 acgtgtccag gccggccacc tgattaacgc accgatgcaa aagctctcgg gtggcgaaac
    37561 gcagcgtgta ctgttagcgc gagcattgtt aaatcgcccg caattattag tgctggatga
    37621 acccactcaa ggcgtggatg taaatggcca ggtggcgtta tatgacctta ttgaccagtt
    37681 gcgtcgcgaa ctggattgtg gcgttttaat ggtttctcac gatctgcatc tggtgatggc
    37741 aaaaaccgat gaagtgctgt gcctgaatca ccacatttgt tgttccggca caccggaagt
    37801 tgtttccctg catccggagt ttatttcaat gtttggtcct cgtggtgctg aacaactggg
    37861 tatctatcgc catcatcata atcatcgtca cgatttacag ggacgaattg ttttgcgtcg
    37921 gggaaatgat cgctcatgat tgaattatta tttcccggtt ggttagccgg gatcatgctc
    37981 gcctgtgccg cgggtccgct gggttcgttt gtagtctggc gtcgtatgtc ttatttcggt
    38041 gatacgctgg ctcatgcctc attacttggc gtcgcgtttg gtttgttgct ggacgtgaat
    38101 ccattctatg cggtgattgc cgttacgctg ctgctggcgg gcggtctggt atggctggag
    38161 aagcgtccac agctggcgat cgacacgtta ttagggatta tggcgcacag tgccctgtcg
    38221 ctgggcctgg tggtcgttag tctgatgtct aatattcgtg ttgatttgat ggcttacttg
    38281 ttcggtgatt tgctggcagt gacgccggaa gatctcatct ccatcgccat tggcgtggtc
    38341 atcgtggtgg ctattttgtt ctggcaatgg cgcaatttgc tgtcgatgac gattagcccg
    38401 gatctggcgt ttgttgatgg tgtgaaatta cagcgcgtga aattgttatt gatgctggtg
    38461 acggcattga cgattggtgt agcgatgaaa tttgtcggtg cgttgattat tacttcgttg
    38521 ctgattattc ctgcagctac tgcgcgtcgc tttgcccgca cgccggaaca gatggctggt
    38581 gtcgctgttt tggtggggat ggtggcagtg actggcggtt taaccttttc cgcattttac
    38641 gatacgccgg cgggtccgtc ggtggtgcta tgtgcggcac tgttatttat tctcagtatg
    38701 atgaaaaagc aggccagcta atctatcgct gaacacattt gtcggatgcg gcgcgagcgc
    38761 cttatccgac ctacggttcg ctatctctgg ctggcctgat aagacgcgaa cagcgtcgca
    38821 tcaggcacac tgccaatgtc ggatgcggtg cgagcgcctt atctgaccta cggttcgcta
    38881 tctctcgtag gccggataag acgcgaatag cgtcgcatca ggcacactgc caatgtcgga
    38941 tgcgtcgcga gcgccttatc cgagctacgg ttcgctatct ctggtaggcc ggataagacg
    39001 cgaatagcgt cgcatcaggc acactgccaa tttcggatgc ggtgcgagcg accaatccga
    39061 cttacggcat ttctggcggc gttatgccaa agtgattcca cgcccgcgtc gtcgccatac
    39121 gcccacgcgg tgtacgctgc aaaaagcctt gctgaatcaa ataaggttcc agcacatcct
    39181 caatggtttc acgttcttcg ccaatggctg ccgccaggtt atccagacct acaggtccac
    39241 caaagaactt atcgattacc gccagcaaca atttgcggtc catataatcg aaaccttcag
    39301 catcgacatt caacatatcc agcgcctgag cagcgatatc tgccgagatg gtgccatcgt
    39361 gcttcacttc ggcgaaatca cgcactcgac gcagcagacg gttggcaatg cgcggcgtac
    39421 cgcgagcgcg acgagcaact tccagcgcgc cgtcatcact catctcaagc cccataaagc
    39481 gtgcgctgcg actgacgata tattgcagat ccggcacctg ataaaactcc agacgttgca
    39541 caataccaaa acggtcgcgc aacggtgatg tcagcgaacc tgcgcgcgtg gttgcaccaa
    39601 tcagggtaaa cggcggcaaa tcaattttaa tggagcgtgc cgccggacct tcaccaatca
    39661 tgatatccag ttggtagtct tccattgccg ggtacagcac ttcttcaaca actggcgata
    39721 gacggtggat ctcatcaata aacagcacgt catgcggttc aaggttagtg agcatcgcag
    39781 ccaaatcgcc cgccttttcc agcaccggac cagaagtcgt gcgtaaatta acgcccattt
    39841 cattggcgac aatgttggca agcgtagttt tacccaaccc cggaggacca aaaatcaaca
    39901 aatgatcgag ggcatcgccg cgcagtttcg ctgctttgat gaaaatctcc atctgtgaac
    39961 gaacctgcgg ctgaccaaca tactcttcca gtaatttggg gcgaatggcg cgatctgcta
    40021 catcttccgg caaagtggta ccggcagaaa tcagacggtc tgcttcaatc atcctttacc
    40081 tcataacgcg gcgcgtaggg cttcgcgaat taatgtttca ctgctggcgt cagggcgagc
    40141 gattttgctc accatgcggc ttgcttcttg tggtttatag cccagcgcca ccagcgcggc
    40201 aaccgcttcc tgttcagcat cgttggttgc cgggctggca ggcgacgtga gcaccagatc
    40261 ggcggctggc gtaaagagat cgccatgcag acctttaaat cggtctttca tttcaacaat
    40321 caagcgttcg gcggtttttt tgccaatacc cggcagtttc accagcgccc ccacttcttc
    40381 acgctcaacg gcattaacga actgctgcgc tgacattccg gagaggatcg ccagcgccaa
    40441 cttcgggccg acgccgttgg ttttgatcaa ctctttgaac aacgtgcgct cttgtttatt
    40501 gttaaaaccg tacagcagct gcgcgtcttc acgcaccaca aagtgggtga aaacgatcgc
    40561 ttcctgtccc gcttcaggga gttcataaaa acaggtcatc ggcatatgca cttcatagcc
    40621 tacgccgccc acttcaatta acaccagcgg gggttgtttt tcaatgatga tgcctctgag
    40681 tctgcctatc acatgacgct cctgcgtaat gaatcaaaga taatgctgta tgataaaaaa
    40741 atgctggata gatatccagc gaaggatgaa gaaaacttgc gaggtgtctc gatgatctga
    40801 aaaatggcac agtataattt attctacagg ttattttgga agcaaatatt ttaatattac
    40861 atattcagtg aaaaaatgcg taataaaaat atacattgcg cctcctg
//
LOCUS       DAPHJE010000032        40692 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPHJE010000032 DAPHJE010000000
VERSION     DAPHJE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.032"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            276..1808
                     /gene="pntA"
                     /locus_tag="RH222_001684"
     CDS             276..1808
                     /gene="pntA"
                     /locus_tag="RH222_001684"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV3796726.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            1819..3207
                     /gene="pntB"
                     /locus_tag="RH222_001685"
     CDS             1819..3207
                     /gene="pntB"
                     /locus_tag="RH222_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV3796727.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3232..4266)
                     /gene="tqsA"
                     /locus_tag="RH222_001686"
     CDS             complement(3232..4266)
                     /gene="tqsA"
                     /locus_tag="RH222_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV3796728.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            4678..5043
                     /gene="mdtJ"
                     /locus_tag="RH222_001687"
     CDS             4678..5043
                     /gene="mdtJ"
                     /locus_tag="RH222_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV3796729.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIVGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            5030..5359
                     /gene="mdtI"
                     /locus_tag="RH222_001688"
     CDS             5030..5359
                     /gene="mdtI"
                     /locus_tag="RH222_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV3796730.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(5398..6219)
                     /locus_tag="RH222_001689"
     CDS             complement(5398..6219)
                     /locus_tag="RH222_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV3796731.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(6319..6402)
                     /locus_tag="RH222_001690"
     CDS             complement(6319..6402)
                     /locus_tag="RH222_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796732.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(6495..6830)
                     /locus_tag="RH222_001691"
     CDS             complement(6495..6830)
                     /locus_tag="RH222_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV3796733.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            complement(7227..8480)
                     /locus_tag="RH222_001692"
     CDS             complement(7227..8480)
                     /locus_tag="RH222_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796734.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            8587..9480
                     /locus_tag="RH222_001693"
     CDS             8587..9480
                     /locus_tag="RH222_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3796735.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            9615..10835
                     /gene="mlc"
                     /locus_tag="RH222_001694"
                     /gene_synonym="dgsA"
     CDS             9615..10835
                     /gene="mlc"
                     /locus_tag="RH222_001694"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV3796736.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            10960..11655
                     /gene="bioD"
                     /locus_tag="RH222_001695"
     CDS             10960..11655
                     /gene="bioD"
                     /locus_tag="RH222_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV3796737.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(11608..12900)
                     /gene="clcB"
                     /locus_tag="RH222_001696"
     CDS             complement(11608..12900)
                     /gene="clcB"
                     /locus_tag="RH222_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV3796738.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13059..13673)
                     /gene="dmsD"
                     /locus_tag="RH222_001697"
     CDS             complement(13059..13673)
                     /gene="dmsD"
                     /locus_tag="RH222_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV3796739.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(13716..14570)
                     /locus_tag="RH222_001698"
     CDS             complement(13716..14570)
                     /locus_tag="RH222_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV3796740.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(14572..15189)
                     /gene="dmsB"
                     /locus_tag="RH222_001699"
     CDS             complement(14572..15189)
                     /gene="dmsB"
                     /locus_tag="RH222_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV3796741.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(15200..17623)
                     /locus_tag="RH222_001700"
     CDS             complement(15200..17623)
                     /locus_tag="RH222_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV3796742.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDCVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREAPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(17684..20110)
                     /locus_tag="RH222_001701"
     CDS             complement(17684..20110)
                     /locus_tag="RH222_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV3796743.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(20309..20614)
                     /locus_tag="RH222_001702"
     CDS             complement(20309..20614)
                     /locus_tag="RH222_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV3796744.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            20722..21432
                     /locus_tag="RH222_001703"
     CDS             20722..21432
                     /locus_tag="RH222_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV3796745.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(21435..21995)
                     /gene="speG"
                     /locus_tag="RH222_001704"
     CDS             complement(21435..21995)
                     /gene="speG"
                     /locus_tag="RH222_001704"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV3796746.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(22030..22371)
                     /locus_tag="RH222_001705"
     CDS             complement(22030..22371)
                     /locus_tag="RH222_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV3796747.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            22506..22832
                     /locus_tag="RH222_001706"
     CDS             22506..22832
                     /locus_tag="RH222_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV3796748.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            23038..24252
                     /gene="rspA"
                     /locus_tag="RH222_001707"
     CDS             23038..24252
                     /gene="rspA"
                     /locus_tag="RH222_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV3796749.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            24264..25283
                     /locus_tag="RH222_001708"
     CDS             24264..25283
                     /locus_tag="RH222_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV3796750.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            25341..25451
                     /locus_tag="RH222_001709"
     CDS             25341..25451
                     /locus_tag="RH222_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV3796751.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(25471..26751)
                     /locus_tag="RH222_001710"
     CDS             complement(25471..26751)
                     /locus_tag="RH222_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3796752.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(26786..27037)
                     /locus_tag="RH222_001711"
     CDS             complement(26786..27037)
                     /locus_tag="RH222_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV3796753.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(27110..29581)
                     /locus_tag="RH222_001712"
     CDS             complement(27110..29581)
                     /locus_tag="RH222_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV3796754.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPNETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPVTKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMQGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRACDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(29675..29866)
                     /locus_tag="RH222_001713"
     CDS             complement(29675..29866)
                     /locus_tag="RH222_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HDV3796755.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVINQDNIEISAGL"
     gene            complement(29863..30051)
                     /gene="dicB"
                     /locus_tag="RH222_001714"
                     /gene_synonym="ftsT"
     CDS             complement(29863..30051)
                     /gene="dicB"
                     /locus_tag="RH222_001714"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV3796756.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(30538..31113)
                     /locus_tag="RH222_001715"
     CDS             complement(30538..31113)
                     /locus_tag="RH222_001715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796757.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRANKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            complement(31115..31270)
                     /locus_tag="RH222_001716"
     CDS             complement(31115..31270)
                     /locus_tag="RH222_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV3796758.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(31439..31846)
                     /locus_tag="RH222_001717"
     CDS             complement(31439..31846)
                     /locus_tag="RH222_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3796759.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            31926..32153
                     /locus_tag="RH222_001718"
     CDS             31926..32153
                     /locus_tag="RH222_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3796760.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            32137..32658
                     /locus_tag="RH222_001719"
     CDS             32137..32658
                     /locus_tag="RH222_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796761.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            32639..33604
                     /locus_tag="RH222_001720"
     CDS             32639..33604
                     /locus_tag="RH222_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796762.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDPTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRHPGAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            33645..34046
                     /locus_tag="RH222_001721"
     CDS             33645..34046
                     /locus_tag="RH222_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV3796763.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVVDPDVIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(34265..36052)
                     /locus_tag="RH222_001722"
     CDS             complement(34265..36052)
                     /locus_tag="RH222_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HDV3796764.1"
                     /translation="MDDINISLDELNTIDLSPLIQSKLPVTCFDITKYLSSLGDGRAE
                     IRLLTHICGFHFRPENPQVPFGPCFQSSQGRSAIPDDINGLSLEVLSQFCPTIELPEL
                     QARIADTLWVRKIGGIRFPLLAVRAYYASSIAIMTSQGTWVSALERLERALRLCCFFR
                     KHTDFRDEFDQLSAHLLAEYERTREQGDSPYPLRLLQLDIDCKVSEPSFIAQELLFLT
                     KNYLAQKLFSFAVDACKTAIPIANRCCDRDTQFEFWRLLADTHLEESKFQDGGMISAA
                     CMQNAIEALANIPGTRIERLALYEEMRDYQIESRHQMSILQSPPQDISEIVHQAKSRV
                     VGRDLFDMVFRLAMLVCRPTSIERLKAQAIEQKANSIAWMFGSTHIDHEGMTLARIPA
                     GLGIDDANGAVIWPIMMTRMRIDHELAVAGQIIPATDEITMKYPISEAFFRDMFINHP
                     FIPFGHEEFFIKGMVCGFNGDFMTACHVLIPQLENSLRYVAKIKGEEPSQLHGDGSQE
                     RNGLKGLLDNPLIIEAFGVDIIGNLQALLVDKIYGDLRNQLAHGYVPAGYYNQPPCIF
                     AWWLVLHILMNPTARYWQATYGQESEAQT"
     gene            36727..36882
                     /gene="hokD"
                     /locus_tag="RH222_001723"
     CDS             36727..36882
                     /gene="hokD"
                     /locus_tag="RH222_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV3796765.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            37099..37350
                     /locus_tag="RH222_001724"
     CDS             37099..37350
                     /locus_tag="RH222_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796766.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFLAFAEE"
     gene            37417..37695
                     /locus_tag="RH222_001725"
     CDS             37417..37695
                     /locus_tag="RH222_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796767.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            37697..38746
                     /locus_tag="RH222_001726"
     CDS             37697..38746
                     /locus_tag="RH222_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV3796768.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQSLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKTPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            38760..39512
                     /locus_tag="RH222_001727"
     CDS             38760..39512
                     /locus_tag="RH222_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV3796769.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(39687..39889)
                     /gene="cspF"
                     /locus_tag="RH222_001728"
                     /pseudo
     CDS             complement(39687..39889)
                     /gene="cspF"
                     /locus_tag="RH222_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            40188..40403
                     /gene="cspB"
                     /locus_tag="RH222_001729"
     CDS             40188..40403
                     /gene="cspB"
                     /locus_tag="RH222_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HDV3796770.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT        10300 a         9537 c        10191 g        10664 t
ORIGIN      
        1 gtctcgtttt acccgaccgt cgaagacaat tatcagtctt tatccggcgt tctaaggtgt
       61 ttatcccact atcacggctg aatcgttaat attttgcgag ttcacgccga aatactgatt
      121 tttggcgcta gatcacaggc ataattttca gtacgttata gggcgtttgt tactaattta
      181 ttttaacgga gtaacattta gctcgtacat gagcggcttg tgtggctcct gacacaggca
      241 aaccatcatc aataaaaccg atggaaggga atatcatgcg aattggcata ccaagagaac
      301 ggttaaccaa tgaaacccgt gttgcagcaa cgccaaaaac agtggaacag ctgctgaaac
      361 tgggttttac cgtcgcggta gagagcggcg cgggtcaact ggcaagtttt gacgataaag
      421 cgtttgtgca agcgggcgct gaaattgtag aagggaatag cgtctggcag tcagagatca
      481 ttctgaaggt caatgcgccg ttagatgatg aaattgcgtt actgaatcct gggacaacgc
      541 tggtgagttt tatctggcct gcgcagaatc cggaattaat gcaaaaactt gcggaacgta
      601 acgtgaccgt gatggcgatg gactctgtgc cgcgtatctc acgcgcacaa tcgctggacg
      661 cactaagctc gatggcgaac atcgccggtt atcgcgccat tgttgaagcg gcacatgaat
      721 ttgggcgctt ctttaccggg caaattactg cggccgggaa agtgccaccg gcaaaagtga
      781 tggtgattgg tgcgggtgtt gcaggtctgg ccgccattgg cgcagcaaac agtctcggcg
      841 cgattgtgcg tgcattcgac acccgcccgg aagtgaaaga acaagttcaa agtatgggcg
      901 cggaattcct cgagctggat tttaaagagg aagcgggcag cggcgatggc tatgccaaag
      961 tgatgtcgga cgcgttcatc aaagcggaaa tggaactctt tgccgcccag gcaaaagagg
     1021 tcgatatcat tgtcaccacc gcgcttattc caggcaaacc agcgccgaag ctaattaccc
     1081 gtgaaatggt tgactccatg aaggcgggca gtgtgattgt cgacctggca gcccaaaacg
     1141 gcggcaactg tgaatacacc gtgccgggtg aaatcttcac tacggaaaat ggtgtcaaag
     1201 tgattggtta taccgatctt ccgggccgtc tgccgacgca atcctcacag ctttacggta
     1261 ctaacctcgt taatctgctg aaactgttgt gcaaagagaa agacggcaat atcactgttg
     1321 attttgatga tgtggtgatt cgcggcgtga ccgtgatccg tgcgggcgaa attacctggc
     1381 cggcaccgcc gattcaggta tcagctcagc cgcaggcggc acaaaaagcg gcaccggaag
     1441 tgaaaactga ggaaaaatgt gcctgctcac cgtggcgtaa atacgcattg atggcgctgg
     1501 caatcatcct tttcggttgg ctggcaagcg ttgcgccgaa agagtttctt gggcacttca
     1561 ccgttttcgc gctggcctgc gttgtcggtt attacgtggt gtggaatgta tcgcatgcgc
     1621 tgcatacacc gttgatgtcg gtcaccaacg cgatttcagg gattattgtt gtcggagcac
     1681 tgttgcagat tggccagggc ggctgggtta gcttccttag ttttatcgcg gtgcttatag
     1741 ccagcattaa tattttcggt ggcttcaccg tgactcagcg catgctgaaa atgttccgca
     1801 aaaattaagg ggtaacatat gtctggagga ttagttacag ctgcatacat tgttgccgcg
     1861 atcctgttta tcttcagtct ggccggtctt tcgaaacatg aaacgtctcg ccagggtaac
     1921 aacttcggta tcgccgggat ggcgattgcg ttaatcgcaa ccatttttgg accggatacg
     1981 ggtaatgttg gctggatctt gctggcgatg gtcattggtg gggcaattgg tatccgtctg
     2041 gcgaagaaag ttgaaatgac cgaaatgcca gaactggtgg cgatcctgca tagcttcgtg
     2101 ggtctggcgg cagtgctggt tggctttaac agctatctgc atcatgacgc gggaatggca
     2161 ccgattctgg tcaatattca cctgacggaa gtgttcctcg gtatcttcat cggggcggta
     2221 acgttcacgg gttcggtggt ggcgttcggc aaactgtgtg gcaagatttc gtctaaaccg
     2281 ttgatgctgc caaaccgtca caaaatgaac ctggcggctc tggtcgtttc cttcctgctg
     2341 ctgattgtat ttgttcgcac ggatagcgtc ggcctgcaag tgctggcatt gctgataatg
     2401 accgccattg cgctggtatt cggctggcat ttagtcgcct ccatcggtgg tgcagatatg
     2461 ccagtggtgg tgtcgatgct gaactcgtac tccggctggg cggctgcggc tgcgggcttt
     2521 atgctcagca acgacctgct gattgtgacc ggtgcgctgg tcggttcttc gggggctatc
     2581 ctttcttaca ttatgtgtaa ggcgatgaac cgttccttta tcagcgttat tgcgggtggt
     2641 ttcggcaccg acggctcttc tactggtgat gatcaggaag tgggcgagca ccgcgaaatc
     2701 actgcagaag agacagcgga actgctgaaa aactcccatt cagtgatcat tactccgggg
     2761 tacggcatgg cagttgcgca ggcgcaatat cctgtcgctg aaattactga gaaattgcgc
     2821 gctcgtggta ttaatgtgcg tttcggtatc cacccggttg cggggcgttt gcctggacat
     2881 atgaacgtat tgctggctga agcaaaagta ccgtatgaca tcgtgctgga aatggacgag
     2941 atcaacgatg actttgctga taccgatacc gtactggtga ttggtgctaa cgatacggtt
     3001 aacccggcgg cgcaggatga tccgaagagt cctattgctg gtatgcctgt gctggaagtg
     3061 tggaaagcgc agaacgtgat tgtctttaaa cgttcgatga acactggcta tgctggtgtg
     3121 caaaacccgc tgttcttcaa ggaaaacacc cacatgctgt ttggtgacgc caaagccagc
     3181 gtggatgcaa tcctgaaagc tctgtaaccc tgacggcctc tgcggaggcc gtcactcttt
     3241 attgagatcg cttaacagaa cggcgatgct ttgacctccc gctgtttgtt caagcgcaat
     3301 tttgacaata attgtcaacg gcacggaaag cagcataccc accggtccta acaaccatcc
     3361 ccaaaaaatc aacgacaaaa ataccaccaa tgtcgaaagc cccagcccac gtcccatgat
     3421 gcgcggctcc agaatattgc cgaagaccag attaatcagc agatatcccg ccagcaccag
     3481 caacgcttcg tagaagccat taaacaccag tacctgagcg atagggggga ttgccgcgag
     3541 aactgaacca atattaggga tgtaattaag cgcaaaggcc agcaatcccc agacaaaagc
     3601 gaagcgaaca tcgagtgcgg cgagcatcgc ccaggcgacc aggccggtga tgatgctgat
     3661 ggctgttttc agcaccagat aatgagaaac actgtcaatc gcacgttgaa tcgccgccat
     3721 cccttcaacc ggacgcgcca tcatttgctg aaattttccg ggcaattgtg gcacttcgag
     3781 cagcataaac agcaccgtca gcagcaataa aaatattgat gacatggcat tagataactg
     3841 cgtcaataag ttggtgagca acgtcatcgc cgcgttcgga tcaatgtaat gcgccagttg
     3901 gtcaactgag acgtcaatcc ctacgcgttg caacaacggc tcaagagctt gcagcggcgt
     3961 cataatagag ttgcgatatt gcggtaacgt ccgcgtcaac tcgttgagcg cggaacccag
     4021 ataagctaac agcaacacca tcgccatcac gatgatggtc atcaaaatcg acaccgccag
     4081 tacacgcggc actcgccagc ggaccatgtg ttgcaccagc gggttaagaa taacagcaat
     4141 aaataatgcg agaataaacg gcacgatgat ctcggcggca aaacggatac cgcagagaat
     4201 aatgaccagc attcccaaca taatgacgat ttttaggcca ttgagcgtga tgatcggctt
     4261 tgccatgctt gtccccgttt tttcttcctt tgctaataat aatgtctttt tcgctccgtc
     4321 atcataacga aaatcaaacc gctcaggtaa agaagtgaaa atattttgag ttaattctta
     4381 agctatgata caaatcaggc gtgttcaact accgaggaca attatcatcc gcgatgacga
     4441 gaagcaacac tgcggataat tgtaatatta tggacaattt gttcaggacg ttattttcaa
     4501 catttaccca tcttcgtacc tcatccacta tcttgctcgt aggtgagcag cactggcgca
     4561 acgcgcttta gtcctttctt ctgcctaagc ttgccctatg cgcggggctt gaccacttta
     4621 ctttggtttc gctgaattaa gcgaaaatta aaataattct cttgcaggag aaggacaatg
     4681 tatatttatt ggattttatt aggcctggct attgctacag aaattaccgg tacgctgtca
     4741 atgaaatggg cgagcgtcag tgagggaaat ggcggcttta ttttaatgct ggtgatgatt
     4801 tctctgtcgt atatatttct ctctttcgcc gttaaaaaga tcgccttagg cgtagcttat
     4861 gcgctgtggg aaggtatcgg tattttattt attaccttgt ttagcgtttt gttattcgac
     4921 gaaagtttat cgctgatgaa aattgtcggg ttaaccaccc tggtcgccgg gattgtgttg
     4981 atcaaatcag gtacccgtaa agcgcgtaaa cctgaactgg aggtgaacca tggcgcagtt
     5041 tgaatgggtt cacgccgcct ggctggcatt ggcaatcgtg ctggaaatcg ttgctaacgt
     5101 ctttttgaaa ttttctgacg gctttcgtcg caaaatattt ggcttgctct ccctggcggc
     5161 ggtgctggct gcctttagtg cgctttctca ggccgtaaaa gggatcgact tgtctgtcgc
     5221 ttatgcattg tggggcgggt ttggtattgc cgccacgtta gccgcaggtt ggatcttgtt
     5281 tggtcaacgg ttaaatcgta aaggctggat tggcctggtc ttgctgttgg ctggaatgat
     5341 catggtgaaa cttgcctgat gaacacgctg cccgcactgt cgggcagcgt ttgaacatta
     5401 tttttgcgac agttgatcca gcttgtcgcg aaaaccggta acagaaatgg cccggttatc
     5461 ggcgcgccag cgatctttcg cggcaggagc cgaactttgc accccaatta attgccagcc
     5521 gtcatcggta tgcaacatca gaggcgaacc gctgtcaccc ggcaaggtat cgcactgatg
     5581 tgacatcacc gacgtttgcg cccagccagt cacttcacag ttttgatgac tgtataacgt
     5641 atcgagatga tcttcaggat atcccgcctg agtcacttta cgaccagccg attttaatgc
     5701 ggcagtaagc gcggctttat ctccctcaaa taacggcaac ggtgtaatgc cagaaggggg
     5761 attacgcagc acaatcaagc cgaagtccca tggtgcagct gcgggaggaa caatccaacc
     5821 atccccatct gcttttaacc gctttcccag tgtcggatca acgcggcctt ctatgtcgtg
     5881 gatgtcatag cgccaaagac ctttatttga cacaaaacgc agcgctaccg ccttatccgc
     5941 tttaccctta ggcggcgtca ataaacagtg tcctgccgtt aatgccagat tgggtgcaat
     6001 cagcgtcgcc gtacataaat tgccgctggc cgtttccagt tgcccaaccg catcccacgg
     6061 tgattgggtc gtgtcattca ctggcacacg atcatcatga ccaaaaaaca gggtgctgac
     6121 ctcatcgttt gccgatttgg caacgtctgg tttatctgca aacacaaaag cagacgtcaa
     6181 actaattgca cccaacacta cagcaatggt tgtacgcata tcacactctg gtgggtaatt
     6241 atgattatta aaagcaaacc ctcataaata ctatagacgg gacgggatga aagtgggagt
     6301 aaaatcagat aactacagtc aggaaagata aaaacgggcg gcaatcagtg cgcataaaat
     6361 aaggatgata agaatgaact caaagcgata acggcccacc atactaccct ccgctaaagg
     6421 cggtgctaaa cgttacccgc ttagcaccgt aattttcagg cgcgaggggg cgcgccagca
     6481 ttactgttga aaacttacgc tgcgggttgt gcagcaggtt ttgccggttg ctgatggctg
     6541 tgtttcccgg cgtgtttctt cgctgcctgc gctttttgtt caggggcttt ctgttcagct
     6601 ttcgtatttt tatgatgctt tttagccgcc tgcgctttct gggcaggtgc tgctttatgc
     6661 tgttttttat gatgtgtcgt tttcgccggc gctgctttgg tggtcgtcgc agtcggagca
     6721 ggtgtggtcg cagtctctgc agcaaaggcg gcagaagaca gacccatagc agcggcaaca
     6781 accagagcta atactttttt cattgtcata ccctcaattt gttttttcat ttaaccccac
     6841 tgcggggccg ttgaaataac tatatccctg gaaatggcag acttccgtga gtggttggtt
     6901 tcagcgtgta acgatatgta caaacgctga ataaattaca gcgttgataa taacgtattg
     6961 tgacttaagg gaaatttagc taccaataat agtagtcttg atcgggttaa ctattttact
     7021 acttacaaag cggtagaata actgcgcatc agtaataaat gacacacagc aaaatgaatc
     7081 cgtttatttg ggtacttata atcctgatga cgctagacgc gctgcgggaa ttggctggcg
     7141 cttcgtctat tttaggatgg ctattaacgt tggtttgagc aagcaataag tccggacgtg
     7201 tagttaccgc agtccgggct tatttttcag gcgtgcagac gacgatgcaa acgcgtcccg
     7261 accagcagag caatgaccag catcagagca ataaatgcgc cgacgccgtt ccagccatag
     7321 ttatgccaga aaacaccacc cagcgtcccg gcaatactcg accccagata gtaactgaac
     7381 agatacagcg aggaggcctg gcctttagcg cgttttgcgc gggggccaat ccagctgctg
     7441 gcaactgagt gggctgcgaa gaatcctgct gagaagagta acattccggc aaagatcagc
     7501 cacagcgagc tgaataaggt catcagtaaa ccaaacagca taacccccgt cgaaaacaac
     7561 atcaccggac cgcgcccgta gcgggtggtc atggttccgg ctttgggtga gctccatgta
     7621 ccggtcaaat aagccagcga taataagcca accacggcct gactgacatg ccagggggag
     7681 agcatcaacc gatatccgat gtaattaaac agcgtgacga acgaccccat cagcaaaaag
     7741 ccttctgcga acaataacgg taatccccgg tcacgccagt gcagacgaaa gttgataaac
     7801 aacgtcttag gtcgcagcga agtcgggcga aaatggcgtg attcagggag gattttccag
     7861 aacatcaacg ccgaggctag cgcgaaacaa ccgattgccg ccagagcaat tcgccagttg
     7921 aaaaagtccg tgaagacacc actaattaag cgcccgctca tgccgccaat tgagttgccg
     7981 ctgatataca accccatcga aaaggccacg aaactggggt ggatttcctc gctaagataa
     8041 gtcatgccaa cagctgccac gccacttaac gaaagcccaa tcaaggcgcg cataatcaaa
     8101 atgccgtgcc agctggtcat cattgtcgaa agtaacgtac aaatggaggc caacagtagc
     8161 gccgtgacca tcactggttt gcgaccaatg gcatcggata gggggccagt aaacagcaaa
     8221 ccaatagcca acatcgccgt ggaaatggac agtgaaatac tactgttcgc gggcgttaag
     8281 ccaaactcct gcgaaagcac cggaaggata ggctgcacac aatagagaag tgcaaatgtt
     8341 gccagtccgg cagagaacag cgccagggtg acgcgcataa attgcggcgt accgcgttta
     8401 ataaattgat ttggctgaga aatgctttgc ttgtcagtgt cgcttgccgg agcgccatca
     8461 acagttgtag tacggctcac ttgaaatcct tgctaaatat gcctgtggat caggcttata
     8521 catagggtag gaaaatcgaa ttgttctgtc taatatatta ataatctcaa ataagatgtt
     8581 ttaaatatga atattgaact tcgtcatctg cgttactttg ttgctgttgc ggaagagctg
     8641 cattttgggc gcgcggctgc ccgcctgaat atttcgcaac cgccgctaag tcagcagatt
     8701 caggcactgg agcaacaaat cggtgcccga ctgctggcac gaactaatcg cagtgtattg
     8761 ctgacggcag caggaaaaca gtttcttgca gatagtcggc aaatcctgtc tatggtggat
     8821 gacgctgccg ctcgcgctga aagactgcat cagggtgaag cgggggagtt gcgtattggt
     8881 tttacttcgt ccgctccttt tattcgggcg gtgtctgata cgttatcgct gtttcgccgt
     8941 gattatcccg atgtccattt acaaacccgc gaaatgaaca ctcgtgagca aatcgctccg
     9001 ctcattgaag gaacgctgga tatgggattg ctgcgtaaca cagcgttacc ggagacgctt
     9061 gaacacgcag tcatcgtcca tgaaccgctt atggcgatga tcccgcacga tcatcccctg
     9121 gcaaataacc cgaatgtaac gctggctgaa ctggcgaaag aacccttcgt cttttttgat
     9181 ccgcacgtcg ggacagggct gtatgacgat attctcgggc tgatgcgacg ttaccatttg
     9241 acgcccgtca tcactcagga ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc
     9301 ggtctgggtg tttcaatttt gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc
     9361 tgggtgccga ttgcagaaga ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat
     9421 catgaacaaa gtccagctgc gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga
     9481 gggaaatttc agcgaaaaag cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat
     9541 ttgacgacac gtattgaagt gcttcaccat agcctacaga ttatttcgga gcgcgaaaat
     9601 atagggagta tgcggtggtt gctgaaaacc agcctgggca cattgatcaa ataaagcaga
     9661 ccaacgcggg cgcggtttat cgcctgattg atcagcttgg tccagtctcg cgtatcgatc
     9721 tttcccgtct ggcgcaactg gctcctgcca gtatcactaa aattgtccgt gagatgctcg
     9781 aagcacacct ggtgcaagag ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg
     9841 tggggctggt ggttgaaact gaagcctggc actatctttc tctgcgcatt agtcgcgggg
     9901 agattttcct tgctctgcgc gatctgagca gcaaactggt ggtggaagag tcgcaggaac
     9961 tggcgttaaa agatgactcg ccattgctgg atcgtatcat ttcccatatc gatcagtttt
    10021 ttatccgcca ccagaaaaaa cttgagcgtc taacttcgat tgccataacc ctgccgggaa
    10081 ttattgatac ggaaaatggc attgtacatc gcatgccgtt ctacgaggat gtaaaagaga
    10141 tgccgctcgg cgaggctctg gagcagcata ccggcgttcc ggtttatatt cagcatgata
    10201 tcagcgcatg gacgatggca gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga
    10261 ttcaggtggt tatcgatcac aacgtggggg cgggcgtcat taccgatggt catctgctac
    10321 acgcaggcag cagtagcctc gtggaaatag gccacacaca ggtcgacccg tatgggaaac
    10381 gttgttattg cgggaatcac ggctgcctcg aaaccatcgc cagtgtggac agtatacttg
    10441 agctggcaca gctgcgtctc aatcaatcca tgagctcgat gttacatggg caaccgttaa
    10501 ccgtggactc attgtgtcag gcggcattgc gcggcgatct gctggcaaaa gacatcatta
    10561 ccggggtggg cgcgcatgtg gggcgcattc ttgccatcat ggtgaattta tttaacccac
    10621 aaaaaatact gattggctca ccgttaagta aagcggcaga tatcctcttc ccggtcatct
    10681 cagacagcat ccgtcagcag gcccttcctg cgtatagtca gcacattagc gttgagagta
    10741 ctcagttttc taaccagggc acgatggcag gcgctgcgct ggtaaaagac gcgatgtata
    10801 acggttcttt gttgattcgt ctgttgcagg gttaacattt tttaactgtt ctaccaaaat
    10861 ttgcgctatc tcaatttggg ccaggaaagc ataacttaga ctttcaaggt taattatttt
    10921 cctggtttat atttgtgaag cataacggtg gagttagtga tgctgaagcg tttctttatt
    10981 accggtacag acacttctgt agggaaaacg gtggtttccc gcgcattgct acaagcgtta
    11041 gcctcccagg gaaaaacggt tgcgggatat aaacccgtag cgaaggggag caaagagaca
    11101 cccgaagggc tgcgtaataa agatgccctg gtgttgcaga gtgtttcaac catcgaactg
    11161 ccttatgaag cagttaatcc tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt
    11221 tgcccaatca attacaccct catttcaaac ggcctggcaa acctgaccga aaaagtcgat
    11281 catgtcgtgg tagaagggac tggcggctgg cgcagtctga tgaatgattt gcgtccactc
    11341 tctgaatggg tggtgcagga acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt
    11401 tgcattaacc atgcactgct aacagctcag gcgattgcca acgacgggct gccgctcatt
    11461 ggctgggtgg ctaaccgaat caacccagga ctggcgcatt atgcggaaat cattgatgtg
    11521 ctcggtaaaa aacttccggc accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa
    11581 cagcgtgaac tggggcaata catccgctta gctatgctgc gcagtgtgct ggcggtagat
    11641 agagtcacgg tgtaacgtcc gcgagattac cgacgcaatt acgcaggcaa tcaataaacc
    11701 ggggagtagc tgatactccc cggtcatttc acatatcatc aacgtcgaca taatcggcgc
    11761 gtgcgtggtt gccgccaaca gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc
    11821 ttcgccatca gggaaccata atcccaggct acgaccatac aacatgccaa tggcaagacc
    11881 gataaatagc gtcggtgtaa agacaccacc gggtgcgccg gaaccgctac tcgccagcac
    11941 ggcacacagt ttacaaagga agatcccggc aatgatcatt aacagtggcg gggcggttaa
    12001 aaaggattgt acggtgctat agccgttgcc ccacactgca ggtgtgaaca gggaaagcag
    12061 acccacgatc aacccgccta gtgccagttg ccagggcggc gcaagtttga gacccacaaa
    12121 tccacgatga caggcgttca ttaacgttaa caacagtggt ccgcacagac ctgccagcac
    12181 acctgtactg ataatcagcg catagtcacg agcctgaacc gtcactgaga gttgtacgtt
    12241 gtagagtaac gcgtcgctat gattaatcag attgctaacc agcaatgcca cgacggcgga
    12301 aataatcacc gggccgagag aggccaacat catagtgcca aacagcactt cggctataaa
    12361 taaactgcca gcaagcgggg cacgataggc cgcagccatt cccgccgcgg ccccacaggc
    12421 gatccataat ttccactcct ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag
    12481 ggcagctaaa agaatcatcg caccttcgcg accaattgca ctgccgctgg ttactaccag
    12541 cagcgaggca agcgatttaa ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa
    12601 cgcttccatg taatcggtcg gcgcatgagg gcgttgttgg gtaaatttct gccagcccat
    12661 cagcaacaaa cccgccgcca gtccgccgag cgccggagtt agcagccgtc gccaggggga
    12721 aaggtttgtc gctgcattga ccagactgcc ggagtcatta ttgaggaaca accactccag
    12781 tagcagcatc gcatgatgaa acccggcaac ggcaaaggcc gcgagaatac cgacgactgt
    12841 tgcgataagc agacggcgga acatagtgcg cagatccgga tagatgtgta gatgatgcat
    12901 gagcagaaat gggcaatgaa acacgggcga ctattttgcg acaaaagagg ccagccgcaa
    12961 aagcagtgcg tgctttaaga gataaaaaaa cgtgacactg taacctatta ttgccggatt
    13021 gcgcgtaatc gtcaccatcc ggcaatatta cggtgatcct atcgaaacag cggtttaacc
    13081 gcgacaggaa tcaacagttg tgattgccat tgcgccagcg ttaagcgagc cagttcaccc
    13141 agtgcacggt agaaagggtg ttctgctttt tcgataaaaa catcaagaaa acgtgttgac
    13201 cacggaaaaa ggtgccatgc cagcaattct tcgcattctg tctggcgacc attctccgcc
    13261 aaccacgcag ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt
    13321 tcaaactgaa tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac
    13381 agcacagatt cgcgatccag ccagaccgac ccccacggcg gagacggcag tgcccacggg
    13441 ccgacaaaca aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc
    13501 gtcaccagcg gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcactg
    13561 gtgagtaccg caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg
    13621 cccaacacgc gcgccgcgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac
    13681 atcctgaaat gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctacc
    13741 gtcatgtgca ggccataaaa gagcacacgc ccgatcatct caccgccaag aatgaggatt
    13801 aaccccagaa taagtccggc aacgtgaggt tcacggcgac gaatgagcgg acaaacccag
    13861 catcctagac ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt
    13921 accagtgcgc tggcttgttg tacggatgag tgaatacacg ccagagacaa accttgcagc
    13981 acaatcaccc cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta
    14041 ttaaatgtaa cgcgagctgc ccgcagaatt gcggcagcaa gtattggacc gctcaacaat
    14101 accgtcagga agaaagccag cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt
    14161 tgatacacgc aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac
    14221 cagagtttcc ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg
    14281 ccaacggcaa aaaatatgga accggcagca atctcgttac tcagaccaga agctccgatt
    14341 cgattcagcg agttaaacgc tcgcagtggc gatcccaggt gcatgacaga ggcaataaac
    14401 ccgatgccca tcaacagcca gaggaaaaac atgccacgga caatacgctg acggtctgcg
    14461 tcatttttgg cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga
    14521 cccaatacgg taaagataac cagtggccat tcatgccatc catttcccat tttacacttc
    14581 ctccggatta gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt
    14641 gataacgata ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt
    14701 gccgtgcttc tggcgcagct cttcaattgg accgaactca agtgcacgca gcgggcagga
    14761 ttcgacgcat atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt
    14821 catgtgccct ttttcagcat tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta
    14881 gcggcagccg atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat
    14941 tgcgccgctg gggcagactt ttgtacatgc tgggtcatcg caatggttac aggagatgga
    15001 gagataataa gcgaacacgt tctggtgcca gacgccatta tcttcctgcc agtcgccgcc
    15061 agcatattca taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca
    15121 cgccagttcg caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg
    15181 tgtggtcatg ggctactctt taaacctttt cgatctggac aagattgctg tgcgacgggt
    15241 ttccctttgc cagcggtgaa gggcggtgag aggtcagaat attgatactg ccgccgtgat
    15301 cgacccggtc accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc
    15361 caggcagaat acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata
    15421 cccgcacggt atcgccatga cggataccgc gagcctgagc atcaatgggg ttgatccaca
    15481 cctcttgtgg gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg
    15541 ccttgtagtg gaagcccgtt aactgcagtg gataggtttt ccgcagggga tcgtcccagc
    15601 catcaaaacc tggcgtataa gctggaaggg gatgaataat ttcatctttt ttcaattccc
    15661 aggtatccgc aatcttcgcc agtcgttcag aataaatttc gattttcccc gaaggtgttt
    15721 tcaacgggtt tgcctgggga gcttcacgga atgcgcggaa agcgacgtag tgttcttccg
    15781 ggcatttttt cttaaagatc ccggtcgttt tcatctcctc gtagtcgggc atctcagggt
    15841 tacgttcctt cgttttcgca tggagatatt tgatccattc atgctgactg cgaccttcag
    15901 taaaggtttg ataaacgtct ggtcctaagc gtttggcgac ttcactcagc atccagtaga
    15961 tgggtttgcg ttcaaatttt gcagaggttg cgggttgggc gaggatcaca tagcccatat
    16021 tccctgcaga ttcatgagag ataaggtctt cttgctctgt tggcatcagg tcgggcaaca
    16081 ggatatcgca atacttagcc gaggccgtca tgaagtggtc aatgccaaca atcatctcgc
    16141 acttgctgtc atcctgaagc acctcatggg tgtgattgat gtcgccatgt tgattgatca
    16201 atgtgttact ggcgtagcac cataaaaact tgatggggac atccagtttt tcttttccac
    16261 gaacacaatc acgggtcgcg gtcatttcct taccatggtc gatggcatct gtccatgtaa
    16321 agacggaaat ctgcgtttta acagggttct caagcatcgg gaaccattct acccccagat
    16381 cccagctacc ttcgcgtaca cctgagttgc cgccgtttat gccgacgtta ccggtgagaa
    16441 cggaaagcat ggcaatagcg cgggacgttt gctcgccgtt ggaatgtcgt tgtggccccc
    16501 aaccctgaca aatataagca ggttttgctg aaccgatctc tcgtgccaac tggataattt
    16561 tttctgccgg gatgctggtg atttttgctg cccattccgg cgttttagct atgccgtcag
    16621 gcccttcgcc cagaatatag gctttataat gcgcgttacg tggtgcgttg gcgggcagag
    16681 ttttttcatc gtaaccaaca caatatttgt cgagaaatgg ctgatcgacc atgttttcag
    16741 taatcagtac ccaggcaatc gcacaggcca gtgcgccatc ggtgcctggg cgaatgggca
    16801 gccattcatc ttcacgcccg gcagctgtgt cgttataacg tggatcgatg acgatcatgc
    16861 gtgcgtttga acgttcgcgg gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc
    16921 gcgtttctgc cgggttattt ccgaacataa cgaccagttt cgtattggcg atatcatccg
    16981 ggctgttgcc atcattggca ccgaacatat aactcattgc ggcactgatc tgtgcggtac
    17041 tgtagctgcc atagcgactg agaaaaccac cgcaagagtt catcagacgg tacgggacgt
    17101 ttgagttggt gatgtttccg ccatctacgc ctgttccgta caggacatgt acagcctcat
    17161 tgccgtaatc ttttaggatc cgccgaagat tatcactgat ggtatccagg gcttcgtccc
    17221 aacttatccg ttcaaattta ccttcaccgc gcttgccgac gcgcttcatg ggatatttca
    17281 acctatcagg atgattcatc cgtcggcgga tagagcgccc gcgtaaacac gcgcgaacct
    17341 gatgattacc gtagacgtcg tcacctgtcg tatcagactc cacccagtac acggtgtcat
    17401 ctttcacatg caaacgtaac agacagcggc tcccgcagtt aacggtgcag gaactccaga
    17461 ccgctttctc ttctaccgga gcctctgccg cccggaccat ttgagaaaat ggcagagtga
    17521 aagcactgct tgccagcgca agactgccaa gtgcggaggt tttcatcaga cttctacggc
    17581 tgatttcagc cttcatgagc gcctctgtgg tatggatttt catcattact cacttattgc
    17641 ttttcaaaca aaatgtcatg ccagaattta tggttgtcgt gggttatatt ttttcgatct
    17701 cgaccagatt agtgtgctgc gggtttccct tcgccagtgg tgaagggcgc agagtggtta
    17761 gcgtattcac acagccgcca tggtcgattt tatcgccaga catattggcc tcgtgccagg
    17821 ctccctggcc catagcgcta accccaggga gaatgcgtgg tgtcactttg gctggtagcc
    17881 gaacttcgcc acgatggtta aacacccgca ccatatcgcc gttggcaatc ccacgtttct
    17941 gtgcatctat agggttgatc cacacttcct gacggcaggc tgccttcagg acatcaatgt
    18001 tgccgtaggt cgagtgcgta cgggatttgt aatggaaacc aaacagttgc agtgggaagg
    18061 tactccgttc aggggagtcc cagccttcaa aggtagaggc ataaactggc agtgggctta
    18121 tcacttcatc tttttccagt tcccaggtac gggcaatttc cgccagcttg ctggaataaa
    18181 tttcaatctt accggaaggt gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt
    18241 tgtaggcgac aaaatgacca ttgggatctt tacgcttata gatacccatt tttttcagtt
    18301 cgtcgtaaga cggtaacgcc ggatctttgg caagcatttt ggcgtacaga tgttgtaacc
    18361 attgttcctg cgtgcgacct tcagtgaact tttgatagac gtcaggtcca agacgtttcg
    18421 cgacttcact caggatccag taaatcggtt tacgttcgaa tttttcgctg gtgactggct
    18481 ggaggaaaat gagatatccc atgttaccgg cgtagtcgtt aggaataata tcttcctgct
    18541 caacggtcat caggtctggc agcagaatgt cggcatattt tgccgatgag gtcataaagt
    18601 tttcgatgac cacaatcatt tcgcatttcg attcgtcttg cagaatttca tgcgttttgt
    18661 tgatgtcaga atgctgatta acgagggtat ttcccgcgta gttccagatg aacttaatgg
    18721 gcacatccag tttatctttg ccgcggacgc cgtcgcggat tgccgtcatt tgcggaccat
    18781 gatcgatagc atctgtccag ctgaagcagg agattgacgt tttgaccgga ttatccagca
    18841 ccggcaggcg ttctatggta atggtatagg tcgattcacg cgcgccacta tttccgccgc
    18901 tgatgccgac attgcccgtc aaaataggta acatagcaat agcgcgtgca gtcagttcgc
    18961 cgtttgcctg gcgttgcggc ccccagccct ggcagatata agcgggtttt gctgtgccaa
    19021 tttcacgcgc cagtttgatg atacggtcta ccgggatacc ggtaatttgc gaagcccact
    19081 gcggcgtttt cgctgtgtta tcatcacctt caccaagaat ataggcttta tagtgaccat
    19141 ttttgggtgc atctgcgggt aaggtttttt cgtcatagcc gacgcagtat ttatcgagaa
    19201 aaggttgatc aacgagattt tcgttaatca atacccaggc aatacccgca accagcgcgg
    19261 catcggtgcc cgggcgaata gggagccatt catcttcacg accagcagcc gtatcggtat
    19321 atcgcggatc gataacaatc attttggcgt tcgatttctc gcgcgctttt tcaagaagat
    19381 aagtgatgcc accgccgctc atgcgggttt ctgccgggtt gttaccaaac atcacgacca
    19441 gcttgctgtt ttcaatatcc gtggtgctgt tgccatcatt actgccgtag gtgtagggca
    19501 tggcacagga aatttgcgcg gtgctgtagg agccatactg attgagtgaa ccgccgtagc
    19561 agttcatcag gcgtttgacc gccgaggctg atggcgaaga gcgggtcata ttgccgccaa
    19621 caatccccga agagtactga atatatacag cctcattgcc atattgttcg acggtttttt
    19681 tcaggctact ggcgatagta tccagggctt catcccagct aatccgttcg aatttgcctt
    19741 caccgcgtgt acccacgcgt ttcattgggt aattcaagcg atcgggatga ttaatacgcc
    19801 ggcggatgga gcgaccgcgc aaacaggcgc gtacctgatg gttgccgtac tcatcgctgc
    19861 cggtattgtc agtttccacc caggtcactt cattatcttt aacatgtaga cgaagtgcac
    19921 agcggctacc acagttgacg gaacaggcac cccagatcac tttttcgctg gcctgttgta
    19981 ccgttgccgc tgcactgcgc agggtgaacg gtaaagaaaa accgcctgca gccagcgcca
    20041 gagaacctat cgcggttgat ttaacgagtg ttctgcggct gatgcccacc attcggtcat
    20101 ttttggacat aactcactcc ctgttcttta tcgttatata aatgtttata tattgaatat
    20161 ttagcgcgct aacaatagag ggagtctacc cattttgggt taagaattat taatccatat
    20221 caatagaagg gtatgagtaa taaggtggga ttatgttgta tgttcaaatc gccggatgtg
    20281 tcgtatccgg cgttcagtcg ataatgtatt actgcggttc ggcaggcgcg ccatcctggg
    20341 tagactgcgc gggagcagag acgttaccgc tggtggtgcg ggtatagaga attttatgcg
    20401 tatcattagc gcaatggccg acgacctggg aatcaggctg atcaacctgg tcattgggta
    20461 caatacttaa cgtgaagctg ctttcgggta cgccattatt gataatgcgc tgtgatatat
    20521 cgctctgtat gcgctcacag gatcccggcg cggcgagtac cacgggtgag gcgagggcga
    20581 gcagaagcgc ggcacagcag gttgagagtt tcatcataag ctccttacgc gaagataact
    20641 tctttaagca tagcatttaa cgtgtaaagt actgtatttg ctactatgat ttagaatcat
    20701 ctctactctc tggtgactgt tgtgaaatac aaattactac catgcttact cgcgatattc
    20761 ctcacaggat gtgaccgcac agaggtaaca ctttcattta cccctgagat ggccagtttc
    20821 tctaatgaat tcgattttga tccgctgcgt ggtccggtaa aagatttcac tcagacatta
    20881 atggatgagc aaggtgaagt gacgaaacgt gtttctggga ctttgtcgga agaaggctgt
    20941 tttgattcac tcgaattact ggatctggaa aataataccg tggtcgctct ggtactggac
    21001 gccaattatt accgtgatgc cgagacgctg gagaagagag tacgtttaca gggaaaatgc
    21061 cagctagcag aattaccttc tgccggggtg agttgggaaa ccgatgataa tggcttcgtg
    21121 attaaagcca gcagcaaaca aatgcagatg gaatatcgct atgatgatca gggttatccg
    21181 ctgggtaaaa ccacgaaaag taacgacaaa acattatctg tcagcgccac gccatcaacg
    21241 gatccgatca aaaaattaga ttacacagcg gttactttac tgaataatca acgggttggt
    21301 aatgtaaaac agagctgtga atatgacagt cacgctaatc cggtggactg tcagctaatc
    21361 attgttgatg aaggagtaaa acccgccgtc gaacgggttt acaccatcaa aaatacgatc
    21421 gattattatt aatgctattg tgcggtcggc ttcaggagag tctgacccgg tgttttgtgc
    21481 tctgccagat actgatgctg gaatatacac atgcgaatgg cattacgata ttgaccatta
    21541 ataaagaact cgtgcatcaa ttcaccttca accgaaaagc caagcttgcg gtaaatgtga
    21601 atcgcttttt cattctcttt atcaacgatc agatacagct tatagagatt gagaacggta
    21661 aagccatagt ccattgctaa tttggcggca cgggttgcca gacctttccc ctgatactcc
    21721 ggggagataa ttatctgaaa ttctgcgcgg cgatgaacat ggttaatttc caccagctcc
    21781 accagaccgg ctttttcgcc gtcacattcc accacaaagc gccgttcgct ctgatcgtga
    21841 atatgcttat catacagatc agagagttca acaaaggctt cgtagggttc ctcaaaccag
    21901 taacgcatca cactggcgtt gttgtcgagt tgatgtacat agcgtaaatc ttcacgctcc
    21961 agcgggcgta gcttaacact gtgggcgctt ggcataacgt gtccttacat tccttaaatc
    22021 aataacaggt tagggggtaa taacgcggcc agttcgacgg tccaggcagc gcaaagtatt
    22081 gggctcccag taggcattga tgttggcgct ttgctcacat ttatcgcggt tatcaaaagc
    22141 ggcgtcggct ttatcccact ctttttcagt gcgtttattc actttctggc gcagattgcg
    22201 cgtgtcattc cattgctctt tttccatagc ggcgtgctgg cggctttgtg cactgtcgcc
    22261 agactcaatc accagtttgt tagtttcggc atgaacagtt gtgctcaatg ccagtgcgca
    22321 aggcagcaga atagcgagca ggccgattcg tttgctgaga gtgattttca taattcattc
    22381 cctgtatgaa tgattaaagg tgattctaca ccatccactg cggacgcaaa acgtaccagg
    22441 agggtgttta tattgatgat attatgtcgc cctataacta tacatgatgt caataagaga
    22501 caaagatgat taaaacaacg ttactatttt ttgctactgc gctgtgtgaa attattggat
    22561 gctttctgcc ctggttgtgg ttaaaacgaa acgccagtat ctggctgttg cttccggcgg
    22621 ggatttcact ggcgctgttt gtctggttgt taacgttgca tccagcggcg agtgggcgtg
    22681 tttacgcggc ttatggtggc gtttatgtct gcacggcgtt gatgtggctg cgcgttgtgg
    22741 atggcgtgaa actgactctt tatgactgga cgggtgcgtt gattgcgctt tgcggcatgt
    22801 tgatcattgt tgcgggctgg gggcgcacgt aggaacataa atccatttta tcaataagat
    22861 aagaggaagt gtcagctgac aaaaggtatt ctatttcatc ttttgtcaac cattcacagc
    22921 gcaaatatac gccttttttt gtgatcactc cggctttttt cgatcttcat acttgtatgg
    22981 tagtagctca gttgcgtaga tttcatgcat cacgacaagc gatgcaagga atcgaacatg
    23041 aagatcgtaa aggctgaagt ttttgttacc tgtccggggc gtaatttcgt cacattaaaa
    23101 atcaccactg aggacggtat tacgggcctt ggggatgcca ccctcaatgg acgtgagctt
    23161 tccgtggcct cttatttgca ggatcacctt tgtccgcagc ttattggtcg cgatgcgcac
    23221 cgtatcgaag atatctggca gtttttctat aaaggtgctt actggcgtcg cggtccggtt
    23281 acgatgtcgg ccatttcagc ggttgatatg gcgctgtggg atattaaagc caaagctgcc
    23341 aacatgccgc tttaccagtt actcggcggc gcgtctcgtg aaggggtgat ggtttattgc
    23401 cataccaccg gtcacagtat tgatgaagct ctggatgatt atgcccgtca tcaggagctg
    23461 ggattcaaag ccatccgcgt gcagtgcgga atccctggta tgaaaaccac ctacggcatg
    23521 tcgaaaggta aaggtctggc ttatgaaccc gcaaccaaag gacagtggcc ggaagagcag
    23581 ctgtggtcga cggagaaata cctcgatttc atgccgaaat tgtttgacgc ggtacgtaac
    23641 aagtttggtt ttaatgaaca tttgctgcat gacatgcacc atcgcttaac gcctattgaa
    23701 gcggcgcgct ttggtaaaag cattgaagat tatcgcatgt tctggatgga agacccgacg
    23761 cctgcggaaa accaggaatg cttccgtctc attcgccaac ataccgtcac acccatcgca
    23821 gtgggtgaag tcttcaacag catctgggac tgcaaacaac tgattgaaga gcaactcatc
    23881 gattatatcc gcaccacgct gacccatgca ggcggaatta ccggtatgcg ccggattgcc
    23941 gattttgctt cgctgtatca ggtacgtact ggctcacacg gtccttccga tttgtcacca
    24001 gtctgcatgg ctgcggcgct gcactttgat ctgtgggtcc ccaatttcgg tgtccaggag
    24061 tacatgggtt attccgaaca aatgctcgaa gtcttcccgc acaactggac tttcgataac
    24121 ggctatatgc atccgggaga caaaccgggt cttggcatcg aattcgatga aaagctggcg
    24181 gcgaaatatc cctatgaacc tgcttatctg ccagtcgcac gtctggaaga tggcacgctg
    24241 tggaactggt aaggagtaag gtaatgaaaa gcattttaat tgaaaaaccg aatcaactgt
    24301 cgattatcga acgtgaaata cccaccccgt cagcgggtga agtacgagta aaagtgaaac
    24361 ttgccggaat ttgtggttca gatagccata tttaccgtgg gcataatcct tttgcgaaat
    24421 atccgcgcgt cattggtcat gaattctttg gcgtcattga tgcggtgggt gaaggtgtgg
    24481 aaagcgccag agtcggtgaa cgcgttgctg tcgatccggt ggtcagctgt gggcattgct
    24541 atccgtgctc tataggtaag ccgaacgttt gtacgacact tgctgtatta ggtgtgcacg
    24601 ctgacggtgg tttcagtgaa tatgccgtgg ttccggcaaa aaatgcgtgg aaaattcctg
    24661 aagcagtggc cgatcaatat gcggtgatga tcgaaccttt taccattgcg gctaacgtta
    24721 ccggtcatgg tcaaccgact gaaaatgata ccgttctggt ttatggtgcc ggtccaatcg
    24781 gcctgacgat cgttcaggta ttaaaaggcg tctataacgt taaaaatgtg attgttgccg
    24841 atcgcattga tgaacgactg gaaaaagcga aagagagcgg ggcagactgg gcgattaata
    24901 acagccagac accgcttggc gagattttcg ctgaaaaagg catcaagccg acattaatta
    24961 tcgatgcggc ttgtcatcct tctatcctga aagaggccgt aacgctggct tctccagcgg
    25021 cacgtattgt attgatgggc ttctccagtg aaccgtctga agtgattcag caaggaatta
    25081 ccggaaaaga actctctatt ttctcttcac gcttaaatgc aaataaattt ccggttgtta
    25141 tcgattggtt aagtaaaggg ttaattaaac cagaaaaatt aattacccat acgtttgatt
    25201 tccagcatgt tgctgatgcc attagtttat ttgaacagga tcaaaaacat tgctgcaaag
    25261 tcttactcac tttttctgaa taataccaat aacggcgagt aagtagtacg catcttacct
    25321 cttttttaga gataaccatt atgacaatag aaaaacacga aagaagcact aaggatttgg
    25381 tgaaagcagc agtatcggga tggctgggca ctgcgcttga atttatggat ttcaagagtc
    25441 atgcgtgtta actatttgat aaatattaaa ttaatttttc attgcttcgt tatggggcat
    25501 ggttggggca aactcgctta actgtgtatt taacaaagct acctgtgcat tattgttttc
    25561 agacatccat tttccgtata cctgaaatac catttgcgca tctgcatggc ccatctggtt
    25621 tgctataaat gccgggttag caccagctgt cagcgaccag caggcataag tatgtctcga
    25681 ctgatatgat tttcgatggc ggagtccggc acgttttatc gctgcgtccc acatctgcct
    25741 tattgagtca acggtaaaat ggtcaccata attttttact ctcgctgaca cttcaggttg
    25801 aaaaacaaag gtgcattttt gtttttctgt tctgccatac tctctgaggt gaacatcaat
    25861 gatatgctct ttgctcagtc tcgttaatgt catctgactc cggagagcgt cgattgctgg
    25921 cttaataaga tgaatgaccc gattggttcc cgcctgtgtt tttggtaccg tgaaacggtc
    25981 ttttgctaaa tttctcctga tcatcattgt tccatttttc agatctatgt cctcccatcc
    26041 aagtgcacac agctcaccag ggcgaacgcc agtataaaca gaaacacacc ataaattttt
    26101 tgcttgctga tttctgcacg catcgataag acggataaat tcttcccgcg aaagaggatc
    26161 cggaatggtt cttgattcct ttaatggcga gatcccctta aacggattat ctgccaggta
    26221 accgttatca acaccaaact ggaacacggc gttaagattt gtcatgtaat tatttacagt
    26281 tacagccgat ctccctggtt gtgtaacaat atagttactt ttggggatct ggtatccagt
    26341 cagtaactct ttacgaacct ccagtaattt ttctttatta atcgatgagg caagattttt
    26401 ttcaccgatt atgctcagga tatttttgat gacggcacgg tatgtgttga gtgatgtttt
    26461 ggcgacttca gtttctttca gtgccagaaa tttttcagcc agttctttta tggttaaatc
    26521 ttgtcgggcc tcaccaaatt tttccagatt gcgtgaggag ggaaactgtt ttgcatagtc
    26581 gaaaacacca gtttttattg cgtaacaaac agaggagcgt agttcacctg caacgcgcct
    26641 gttttttgct gtgtcaggaa cccccagatt ttccctgact cttacgcctt tataaacaaa
    26701 ccagatacgt aatttccctc catggttttc cacgcctgtc ggatatttca tttcaacttc
    26761 tctcattagt tagtgtggct tttagtcaag taagatgacg tcttggtctc gctgatgcct
    26821 ggcgctcaat ccagcgatca atttcttcca ggttgtaaaa gcatggactg ttatcccatg
    26881 gcataccgtc atgagcgaca tgcttatatt cccttccttc cataaacgat ttttcccggg
    26941 ccttttttaa cgtacctttt tttattcctt tcagcgcaat taactgctct tcggataccc
    27001 atttgccggg agagacaatc atgattactt cgctcatcga tttctttatc tcttacatca
    27061 gacgagcgcc ggttgcagaa taccagtcac aaccggcgac agttgaacat taagaatcag
    27121 cctgactcgg gatcagtttt tgccagataa ctgaaacgta ttttgcctgg taacgggcgt
    27181 catcaagtgc attatggcgc tcaccttcga atggaatagc cgttctggca tcgaagtcta
    27241 tggctttccc cagctcaacg attgtgcgta catcgcgatc gttgtagtaa cgccacgggc
    27301 aggggatccc ctgccgttcg tatgaacggc gcaaaatcgt gttgtcgaag ttggctccat
    27361 ttccccaaac ctgaacaaaa aattcaccgg agttttcgtc gataaattcc cgcaattgta
    27421 acagtgcatc atctaacggg atttcatcgg tcataatggc agattgcgct tcgcgtgatt
    27481 gcttaagcca ccatttaatg gtgtcccgat caatgactcc gccagcagtt tccagatcga
    27541 tagtcttact aaattccggt cccatatctc cggtttgcgg atcgaaaaat attgcaccta
    27601 ttgagatgat cggggcatca ggattttttc ccatggtttc aaggtcgatc attagatggt
    27661 cacacgccct gctggtggat gtgatttcgt gatgaccgtt caccttaatt gggtgatctg
    27721 ccgtctcgcc agtttcatta tcgctattgt gatgctgatt gccgccagtg ttctccttgt
    27781 gtggatgttc agcgccttgc atttcctccg gatcatcttc ctgaacttca acctgatact
    27841 cttcatcgaa tgtttcctgg tatgttgcgt cgcccatcac cgcgccacaa tcagggcagt
    27901 tgccgccgcc ggtctgaccg caggcggtgc agactttttc cacttcctgt tgcgctactg
    27961 gttcaggctg tttcgttact ggctcgtttt gtaacgcatt tggactgttt tgttccgctt
    28021 tttggtagtt ccgttccgat tcatgctggt tctggttcac agaatcgcgg gtctggatcc
    28081 ccttaaccca tttcggatca ttcgggtcac taatcccttc aacaaattca ccacgtgatg
    28141 cagcaagcaa cttatcagcg tcaggctggc tgatattggc tgcctgcata attttgttta
    28201 cttcgtcagc ggtaactttt atcggctctg gttgttctga atcttcagcg gtatctacat
    28261 tttgcggtaa gcccgtgtat gtgccatttt ttcgggcaaa atattcttct tttgtgattt
    28321 cagtggcgcc agcagccagt gccttatcca gaccagaaag tttgtttgcg cgaccgtatt
    28381 tttctccgtc cttatctgcg aagaggaaat agaacggccc ctcacgctct acagatggtt
    28441 cagcttccgg cgcggtttca ttttttggga tatcagatac ctcagtttcc actgcatcag
    28501 tttgtgtttc tgatgactgg agaacatcaa cagtgcccag gtctgtttct tcattctcaa
    28561 acacgccctt tgtcgtcagg tattcgcaga tatatttgtt cagtgctacg ggatctttgt
    28621 gaatgtcgat cggacgctca cggacaaggc caaaaatagt ctggcggtcg tagcgaaggg
    28681 catcaggctg tttgcgcatt gatgccgaga tacgcttcca gtcttcgcgg tcgttgtcga
    28741 taacttcttt ttttgcccag cgatggatgc tgccgtcaat gtttccggca tccacatcac
    28801 caggccagag agcgtaggcc agttcgtcat ccagtgtttt ccatgtctgc ttgtattcgc
    28861 gatgaatggc agcaatgacc gggctgattt ttcctgttga attttcagtg tactgttgat
    28921 tggctctggc gcgtgcgaga tcaacaacag acgtgtattt tccggtttcc ttgcgttcac
    28981 cttcgcgacg ttttttccag atgcgcatct ctgcctgaat ttcgggccat ttagcaccag
    29041 gaatacattt atgcttaacc cacccaatgg cgtgcaactt aagctccgga tacatggcgt
    29101 taacttctgg cattttcatc aacgcttcaa cgatatgtcc gtcgaatgtt gccatgtctt
    29161 cctgcaacaa ttcctgtgcg ctaataacca tatcaacggt gatgttttca catgtgtcga
    29221 acttaaccat gacagcgttc tgtacttcag gggccagctt gtcaaaagtg acgttcatcg
    29281 gatcggattc agtctcattc gggacaaaag aagcagactc ctcatcccag cggttttcct
    29341 gcatatattc agcatcccat gaatcgaggg cagggcgggg tatgccaggt ttatcctcgc
    29401 agacaataaa tttataagcg cagtcctgag cagccggata atgttccagg aattgccagt
    29461 gaaattttgc tcgagcacgg cgttcgtcgc cagcttcaat ggctgtggct acagccacag
    29521 cgccttcttc ccttgttgcc agttcgtcag gaatagcggc gcaaataaag actttactca
    29581 ttttgtttta acctcatgac agatttaagg atgaacaaat ccctgccatt gctggcatat
    29641 aagaatgaaa ccggatattt attacggaac tgttttaaag acctgccgag atttcgatat
    29701 tatcctggtt aataacttta tcgaccgggt aacagttacc gggaattttc tgttcggttg
    29761 ctgcagtcat acactcctgc attgtcctgt gaacactgac tgcaatatca actggcactc
    29821 cggaaacaag aaaaactgtc agaacaagcg caaatgctga attcattgtg cacatccttt
    29881 tggcatcaga cgtaaacgag ccagcattga aacaatgcat attttattta atagctcccg
    29941 ttcttgtttt ctcttgttaa tggcatcttc agtaaataca gggttactga tagtgacacc
    30001 aatttcaaaa caaccttcag acgtattaac gtttggtaat aacgttttca ttatcgcgtc
    30061 ctcaacaata aattttgtga tgcagagcct ggtgcctcca ggtgacgtta accagttaac
    30121 aattaacgtc ggatacagag aatccaccca taacactgtt tttggtttta actgttccgc
    30181 gtgcgcttag ccgcattcac cgcataacaa aattcacttt aaaaacggcg gcagagcagt
    30241 cacggagtaa aactgatacc gccaaacgtc accagaaaat tgataacaga gggcgttgca
    30301 gcggggttgt cacttaagcg tatggtcaac ctgacaaccc ggtgtcctca acggggaagg
    30361 aataaccccg ccatacttac cgccgcgcca ttttgcgggt tgccacaacc ggaagcgcac
    30421 ggtcgaatta aatttaacga caccgtacag tgagacgaac ttcgccgtgc gctttcgtgt
    30481 tgtgtgcctg cttttaacca cgtcaggcga ggtggtatcc ttaaaatcac cacagtttta
    30541 aggattcatt aagcaatgtc gcaaccacca ataaatccgc ttaagaacat gaaaattgat
    30601 tactggtata aagcgcttac agttgttggc gctgcgttgt ttgtctttaa tggaacgtct
    30661 ttttttgaca gatatcccgt tgttccattg ggttttttgt cctccggcat cttttttatt
    30721 ggtttggggg agtggattaa tcaccctctc aaagtgagat ttattggccc tggagtttgg
    30781 actcgtggat ataatcgttc ttcgtgcgca ctcggtatca tcttcgacat acttggttgt
    30841 ttcctgattg ttacaggagt cgtcaagttc ttctgatgta aaaccgcaaa tggggcacgt
    30901 aacgggaatt ttgaaaagcg tttctccggg ttccagaaca aaattttctg cggtctgatt
    30961 ttgcttctca tatttgtgct ccgcgtcatt gtgagagcac attcttattc tgagtgcctg
    31021 tttaaactca ctgaagctga gagcttcttc gccttcggca aggccttcga agtattcttc
    31081 gtaagccttt tccatgattg tgtcgaaatc catatcactc acctgagttt ctttccagcc
    31141 agcgacgggc accattttcg gttttaaacg ttttgctttt ggtatacgtc atcgcggtga
    31201 acgtgccgtc ctggttgggg aacacgccac ataccagaga ttcgctgttg ccaagatcga
    31261 tagtatccat gctgacctca tttcccctta acgccggggt agcggaactg tttgctgaga
    31321 acaccgtgcg gtgtcttgat ggaaagtaat ttagaataac ctaacatgag aggcaagtgt
    31381 tttttgttag attagtctaa caaaaagagt gggcgcaact aatcacttga aaagaatgtt
    31441 attttattga tttattttta cgcgctttaa gcatttcttc gaagagtttg ttgaagtttt
    31501 ctactcttgc gcgcatttca gacagcaagg cttcctgctc ggaagatgga agagcatcga
    31561 ataattcgat caattctttg tggttgggag ttagctctgt ttccacatga agttcttgtg
    31621 caggcactgg tgccttgtct tcgtcaccaa acattagcca tgtaggtgag cacttcagag
    31681 catccgctaa agcaaacaat cgttttccga ctggctgggt ttcgtctctt tcccattgtg
    31741 aaattgtgac gtgagcaact ccagcgaggc gcgcagcttc tcgttgtgtt aagcgtaatt
    31801 cttttcgtcg cgccagaact cgctggccta gggttcttgt atccatagtt aggtaattct
    31861 aatttttctt gacttagata tcccgcgcac aataatgtta gaaaagtcta acaagagggg
    31921 ctttgatgct taaagttgac gcaattactt tttttggcag caaaacaaag cttgccaatg
    31981 ccgcaggagt gagactggca agtgttgctg cttgggggat actggttcct gaaggtcgcg
    32041 cgatgcgtct acaggaggca tctggcgggg agcttctgta tgatcccaag gtttatgacg
    32101 aatatcgtaa gacgaagcgg gcggggcggt tgaacaatga aaatcactcc tgaacaggct
    32161 cgtgaggctc tggatgcctg gatatgtcga ccaggaatga cacaggagca ggcgacgata
    32221 ttaatcactg aagcattctg ggctttgaaa gagcgcccga acatcgatgt tcagcgtgtc
    32281 acagatgaag gtggcgcggt tgatcagcga gcgcttggcg ttaatcgagt gaagatattc
    32341 gaacgctgga aggctatcga caccagggat aagcgtgaaa agttcacggc gctagtgcct
    32401 gcaattatgg aggctatccg gattaatgat ttcaggttgt atcgtgaaat tagtgacgga
    32461 aaaagcatca cgtacatgat cgccgggtta aacaaagaat atggcgatgt ggtggagtcc
    32521 ggactgcttt ttgctgatcc tgccgtagtg gatcgtgaaa ctgacgaact tatagaaaaa
    32581 gcaattgctt tcaaacttgc gtatcgacag caataccaac aaaaagctgg atggaattat
    32641 gagccttctt tttgctgaac gcccactggt tataaacaca cagcttgcga tgaagattgg
    32701 cttaaatgaa gccattgtgt tgcagcagtt gcattactgg ttgagagata ccaattccgg
    32761 catggaatgt gatggtgttc gctggattta caacacaacg gaacaatggt tggaacagtt
    32821 cccattctgg tcagagtcaa cgttaaagcg cgcgtttgca agtctgaaaa cgctggggct
    32881 tttgcgttgt gaaaagctca ataaatcaaa gcgcgatatg accaatttct acacgattaa
    32941 ctatgggagc gagcttttag atgatggcaa attgagcgaa tccatcggtt caaaatgcgc
    33001 cgctccatca ggtcaaaatg acacgatgga agaggtcaaa atgaaacgct ccattggttc
    33061 aaaacgaccc aatgtcatcg ggtcaaaatg gcccgatgat cctacagaga atacaacaga
    33121 gattactaca gagaataaaa acacttttcg tccggaagct tcgcaaccgg acccgcagac
    33181 ggctgaacag gattttttaa tccggcaccc tggcgcagtt gtgtttagtg cgaaaaaacg
    33241 ccagtggggt agccaggagg atctggcgtg tgcgcagtgg atatgggggc ggatcgtggg
    33301 tctctacgaa caggccgcca gtgatgatgg cgagatcatg cgaccaaaag agcctaactg
    33361 gactgtctgg gccaatgatg tgcgcacaat gcggatgctg gatggcagaa gccacagaca
    33421 aatttgtgaa atgtttggtc gggtacagcg ggatccattc tgggtaaaaa acatcatgag
    33481 cccgtcaaag ctccgcgaaa aatgggacga actggtcatc cgcctggggc gttcacctgt
    33541 acagcgttgt gttaatcata tttctgaacc ggatacagaa attccgcctg gtttcagagg
    33601 ataagttttg atttcaggtc atgaggtaat tttaaggggg acttgtggca aaagttttta
    33661 cacaagaaga gcgggaaaaa attaaagggc aggtggtgga actcgtgcgc cagagcggtc
    33721 gtgagacgtt acggcaactg gaagctaaaa caggtgcgac tagatatctg atgagcgttc
    33781 ttgccagaga gctggtagcc agtggcgatg tatacaactc cggctacggg ttattcccgt
    33841 ctgaacaggc gcgtaaggac tggcaaaacg cccgcaaaaa actctcgagg gcaaaggtga
    33901 agaaaacagc tgtggttgat ccggacgtta tctggtcgtt acctgatgga gaaatacgtc
    33961 gctacgacag tcgcctaaac ataatctgtc gcgagtgccg gatgagtgaa gttatgcagc
    34021 gcatactggc attttatcag ggataatgtt aggtatttta gacgttacta gattaaaaag
    34081 cattagttca ggagtgaatt gacattctca tttttcatgg cacagggtag atctggcgtg
    34141 gttgtccgct ttgtgccaaa agcggacatc gaataatttc agcagcactc aaccttatta
    34201 accaaatcgc ctcaactcat atggacatgc atccggaagt gccaacttcg taaacttatt
    34261 cactttaagt ctgagcctca ctctcttggc cataagtggc ttgccagtat ctcgccgtag
    34321 ggttcattag aatgtgcaat acaagccacc atgcaaaaat gcagggaggt tgattgtagt
    34381 aaccggcagg cacatatccg tgagctagtt gatttcgtaa atcaccatag attttatcta
    34441 ccagtaaggc ctgaagattg ccaattatgt ctacgccgaa agcttcaata atcagtggat
    34501 tatctagtaa tcccttgagt ccatttcgct cttgcgaacc gtccccgtga agctgcgatg
    34561 gttcctcacc tttaatttta gccacatatc tcagactatt ttcgagctgg ggaatcaaca
    34621 cgtggcatgc cgtcataaaa tctccattga aaccgcaaac catacccttg atgaaaaact
    34681 cttcatgccc aaacgggatg aagggatgat tgatgaacat atcgcgaaaa aacgcctccg
    34741 agattggata tttcattgtg atctcatctg tagcaggaat aatttgccca gctactgcca
    34801 actcatggtc gatacgcatt ctcgtcatca ttatgggcca aataactgcc ccatttgcat
    34861 catcaatccc caatcctgcg ggtatgcgag caagtgtcat tccctcatga tcaatatgtg
    34921 ttgatccaaa catccaagct atgctgttgg ccttttgttc tattgcttga gccttaagtc
    34981 tctcaatgct ggttggccga cagaccagca tcgcaagtct gaaaaccata tcaaacaaat
    35041 ctcttccgac tacccgactc ttggcttgat ggacaatctc actaatatct tgagggggag
    35101 actgaaggat agacatctgg tgacgggatt cgatttggta atcacgcatt tcttcataca
    35161 gcgcaagacg ttctattcgg gttcctggaa tattagccag tgcttcaata gcattctgca
    35221 tgcaggctgc cgaaatcata ccgccatctt ggaattttga ctcttccaga tgtgtatcgg
    35281 caagaagccg ccagaattca aactgtgtat ctctgtcaca gcacctattc gcaatcggga
    35341 ttgcggtttt acaggcatcg acggcaaatg aaaataattt ttgagccaga tagttttttg
    35401 tcagaaacag gagttcctgg gcaataaaag atggctcact cactttacag tcgatatcga
    35461 gttgcaggag tcgcagcgga taaggtgaat cgccttgttc tctggtacgt tcatactctg
    35521 ctagtagatg tgccgaaagc tgatcaaatt catccctgaa atcagtatgc tttcgaaaaa
    35581 aacaacacag cctaagcgca cgctctaggc gttctaaagc actaacccac gtcccttgcg
    35641 atgtcataat agctatactg gacgcataat aagcacggac tgccagcaaa ggaaaacgga
    35701 ttccaccaat tttacgaacc caaagtgtat cagcaatacg agcttgaagt tcgggtagct
    35761 ctatcgtcgg gcaaaattga gaaagtacct ccagcgacaa cccgttaatg tcatccggaa
    35821 ttgcagatct accttggctg ctttgaaagc acggaccaaa tggtacctgt ggattttcgg
    35881 gacgaaagtg aaaaccgcaa atatgggtta gcagtcttat ctccgctctt ccatcaccta
    35941 ggctagaaag atactttgtg atatcaaagc aggtaactgg cagcttactc tggattaagg
    36001 gggataaatc aatagtgtta agttcgtcga ggctgatatt aatatcgtcc atgtcgtcac
    36061 cttgatgatt tgttaatgcg ggggcaacat taataaatgt acttgagacc gcaatttgtc
    36121 gatggagcca acatttataa ctatgtgctt ctaccctctg aatttttaca tgtgaattgc
    36181 tagcttgaat agctctatca ttatatacgt ggcactaaat tatggctgtt tttcgctcaa
    36241 agcggactag aaggttagct tgtgtcggac tttgcgtatt aaaagaagtg ctggtggtga
    36301 ctagtggttg tgccccattt ccacagaaaa aatcagagaa actataccca atagttgtat
    36361 tgaatcactg acgagacagc ctcatattca tcaggactgg tctacgtcca atacaggagg
    36421 ttgtggtgct ggttctcaaa tgtgcgctgg ctattacggc tgtaatggcg atttattgtc
    36481 ttgctattgt tcttatggat cgcctttctg actgatttca cattggcgag gtaacggtag
    36541 ttaagtagaa tggctgcggg tgcttgaggc tatctgcctc gggcatgaac accaacggca
    36601 gatagagaaa agccccagtt aacattacgc gtcctgcaag acgcttaaca ttaatctgag
    36661 gccaatttca tgctagacat atgtaggtta gcctcttacg tgccgaaagg caaggagaag
    36721 caggctatga agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc
    36781 atagtgacgg cactggtaac gaggaaagac ctctgcgagg tacgaatccg aaccggccag
    36841 acggaggtcg ctgtcttcac agcttacgaa cctgaggagt aagaaacccg gcgagggaga
    36901 aatccctcgc cacctctgat gtggcaggca tcctcaacgc acccgcactt aacccgcttc
    36961 ggcgggtttt tgtttttatt ttcaacgcgt ttgaagttct ggacggtgcc ggaatagaat
    37021 caaaaatact taagtagcgc gcagggataa gagggatggt cccttaaagg ggagagctaa
    37081 ttatccggaa ggattctgat gatgaacatc gaagaattgc gtaaaatttt ttgtgaagat
    37141 ggcctctatg ctgtgtgcgt tgaaaatgga aatcttgtta gtcattaccg cattatgtgt
    37201 ttgcgaaaga atggggctgc gttaattaat tttgtggatg gtcgagtgac agacggattt
    37261 atcttgcgcg aaggtgagtt tgtcacttca ttacaggcac tgaaagagat cggaataaaa
    37321 gcaggctttt tagcttttgc agaagaataa actcatctac aatcttgcgc ggggctgaac
    37381 tcccgctgag taacaccgtg ccaccggaga aaaccgatgg cacgcaacgt aaaatattac
    37441 aattctgata attcgcccgt tcttgtctgc acgcacgagc ggtattctca cgcattcaag
    37501 tctgaatggt tccaacaccc tccatgcact gaagagcagg ctgaatggat aattcagtgt
    37561 taccgcaggc gcggatacga ggttaagaaa gcccttagcc tcgactaccg tcactggata
    37621 atctcagtca gattgcctta ctccgaacgc ccaccacgtg cgtcccgcac tttccagcaa
    37681 cggatctgga ggtaacgtgc gggtattact tagacctgtt ctggtgcctg agcttgggct
    37741 ggtggtcctt aagccgggcc gtgaatccat acagatattt cataatcctc gagtgctggt
    37801 ggaaccggaa ccaaaaagca tgcgtaatct gccatccgga gtcgttcctg ccgttcgcca
    37861 gtcgctggcg gaagacaaaa cattgctgcc gttttttagt aacgaacggg tgattcgtgc
    37921 tgctggcggt gttggcgcat tgtctgactg gctattacgt catgttacat cctgccagtg
    37981 gcctaatggc gattaccatc actctgaaac agtcattcac cgttatggta ccggcgcaat
    38041 ggtgttgtgt tggcactgcg acaaccaact gcgtgaccag acatcggaat cactggagct
    38101 gcttgctcaa caaaatctga cagcatgggt gattgacgtc atccgtcacg caataagcgg
    38161 tacgcaggag cgggaattat ctttggctga attatcctgg tgggcggtct gcaatcaggt
    38221 ggtggatgca ctacctgagg ctgtatcgcg tcgttcgctg ggattaccag cggaaaaaat
    38281 ctgctcggtg taccgcgaaa gcgacatcgt accgggagag cagaccgcca ccagcatatt
    38341 gaaacaacgc acaaaaaatc ttgcaccgtt gccttacgcc caccagcaac aaaaaacacc
    38401 acaggaaaag acggtggtaa gcatcaccgt tgatccagag tctccggaat ctttcatgaa
    38461 gctgcctaaa cgtcgccgct gggttaagga gaaatacaca cgttgggtta agacacagcc
    38521 gtgtgcttgc tgcggtatgc cagccgacga tccgcatcat ctgattggtc acgggcaggg
    38581 cggaatggga acaaaagcac atgatctctt tgtgttgcct ttgtgcagaa agcatcacaa
    38641 cgagctgcat acggatacag tggcatttga agagaagtat ggctcccaac tggagctgat
    38701 atttcgtttt atcgatcgcg cgctggcaat tggcgtactg gcgtaagtgg agaacgagca
    38761 tgaaccttga agccttacca aaatattact ccccaaaatc tccaaaattg agcgatgacg
    38821 ctccagcgac aggcaccggt tgtttaacaa ttacggatgt aatggcagcg caggggatgg
    38881 tgcagtcgaa agcaccactt gggttggcct tatttctggc aaaagttggt gttcaggacg
    38941 ctcagtttgc gattgaaggc ctgctaaatt acgcgatggc actggataac ccgacattga
    39001 acaaattgag tgaagaaatc cggttacaga ttattcctta cctcgtgaat tttgcctttg
    39061 ctgattactc caggtctgcg gcaagtaagg ctcgctgtga gcattgttca ggtacgggat
    39121 tttataatgt attgcgcgaa gtggtgaaac actacagacg cggggaatct gtaatcaagg
    39181 aagaatgggt gaaggaacta tgtcagcatt gccatggtaa gggcgaagtc agcacagcgt
    39241 gcagagggtg taagggtaaa gggattgttc tggatgaaaa aagaacccgg tttcatggcg
    39301 taccggtata taagatttgt gggcgttgta atggaaaccg gtttagtcgt ttaccgacca
    39361 cgctggcacg acgtcatgtc cagaagctgg taccagacct gaccgattat cagtggtata
    39421 aggggtatgc ggacgtcatt ggtaaactgg taacaaagtg ctggcaggaa gaagcatacg
    39481 cggaagcgca attgaggaag gtgacgagat aaatgatttt tgctgaagat ggcgacatga
    39541 tgtttgcatt tttcaaaaaa catggataag attttctcaa cgatgggctt tgtgtatccg
    39601 acgtttagaa aaaagtagaa aacccgctta taagcgggtt tttgtgcttt aaatggggca
    39661 atagagatat tgaatctcat cccgggataa acattggcag ttgaaggtcc acgcgaacca
    39721 tttatccagc aaaattccac gcgtaatcct gtggtaattt cttctgcatc tcgaagattg
    39781 agagctgaaa cgtgaagctg ggcatcgata cgccatcgga tgggaatata agacctttgc
    39841 tgcttttgta gtcaaagttt ttgacaattc ctgtcatttt aggggacaga aaaactcctt
    39901 aatactgata acctggtgca ccatacacac gttcctggag aaaactactt ttttgatagg
    39961 attgaaggtg gctggatgtc taaaataaac attgcttcat atgttcaact atgagttaat
    40021 gactgcgtcg gtttgaagaa cagacgatat acgaagtagt ttactaaagc agttctcatt
    40081 tcaggtgtta ttcacttatt ccttctttga gtctctccaa ttaagtacga agtcgtttct
    40141 gttatgcaaa ccatttatgc cgaaaggctc aagttaagga atgtagaatg tcaaataaaa
    40201 tgactggttt agtaaaatgg tttaacgctg ataaaggttt cggctttatt tctcctgttg
    40261 atggtagtaa agatgtgttt gtgcattttt ctgcgattca gaatgataat tatcgaacct
    40321 tatttgaagg tcaaaaggtt accttctcta tagagagtgg tgctaaaggt cctgcagcag
    40381 caaatgtcat cattactgat taaaattcat cgctcgtctg tatacgataa cgaagaaggc
    40441 tgatgcctga gtagagatac ggacagagta gtgaatattg gatctcttta ataaaaagta
    40501 aggaggtcca atacatgaaa caatggctag catatttggc aaaatcttaa tcaggaaaag
    40561 tatgctaacc attgtggtga agtgcaggtt tgctgcatga atagtttcac agcagaagct
    40621 aactgctggc atcgcaaaac aaagtgcgta agtggatgac tcccacaaaa agcaccacaa
    40681 tctcaaaccc gc
//
LOCUS       DAPHJE010000033        40308 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPHJE010000033 DAPHJE010000000
VERSION     DAPHJE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.033"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            303..389
                     /gene="leuL"
                     /locus_tag="RH222_001730"
     CDS             303..389
                     /gene="leuL"
                     /locus_tag="RH222_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV3796771.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            482..2053
                     /gene="leuA"
                     /locus_tag="RH222_001731"
     CDS             482..2053
                     /gene="leuA"
                     /locus_tag="RH222_001731"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV3796772.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2053..3144
                     /gene="leuB"
                     /locus_tag="RH222_001732"
     CDS             2053..3144
                     /gene="leuB"
                     /locus_tag="RH222_001732"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV3796773.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3147..4547
                     /gene="leuC"
                     /locus_tag="RH222_001733"
     CDS             3147..4547
                     /gene="leuC"
                     /locus_tag="RH222_001733"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV3796774.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4558..5163
                     /gene="leuD"
                     /locus_tag="RH222_001734"
     CDS             4558..5163
                     /gene="leuD"
                     /locus_tag="RH222_001734"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV3796775.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5212..6390)
                     /gene="setA"
                     /locus_tag="RH222_001735"
     CDS             complement(5212..6390)
                     /gene="setA"
                     /locus_tag="RH222_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDV3796776.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="RH222_001736"
     CDS             complement(6492..6623)
                     /gene="sgrT"
                     /locus_tag="RH222_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV3796777.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6712..8367
                     /gene="sgrR"
                     /locus_tag="RH222_001737"
     CDS             6712..8367
                     /gene="sgrR"
                     /locus_tag="RH222_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV3796778.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8401..8500
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8531..9514
                     /gene="thiB"
                     /locus_tag="RH222_001738"
     CDS             8531..9514
                     /gene="thiB"
                     /locus_tag="RH222_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV3796779.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9490..11100
                     /gene="thiP"
                     /locus_tag="RH222_001739"
     CDS             9490..11100
                     /gene="thiP"
                     /locus_tag="RH222_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV3796780.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11084..11782
                     /gene="thiQ"
                     /locus_tag="RH222_001740"
     CDS             11084..11782
                     /gene="thiQ"
                     /locus_tag="RH222_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV3796781.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11896..12660)
                     /locus_tag="RH222_001741"
     CDS             complement(11896..12660)
                     /locus_tag="RH222_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3796782.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12746..13624)
                     /gene="araC"
                     /locus_tag="RH222_001742"
     CDS             complement(12746..13624)
                     /gene="araC"
                     /locus_tag="RH222_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV3796783.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13963..15663
                     /gene="araB"
                     /locus_tag="RH222_001743"
     CDS             13963..15663
                     /gene="araB"
                     /locus_tag="RH222_001743"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV3796784.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15674..17176
                     /gene="araA"
                     /locus_tag="RH222_001744"
     CDS             15674..17176
                     /gene="araA"
                     /locus_tag="RH222_001744"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV3796785.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17376..18071
                     /gene="araD"
                     /locus_tag="RH222_001745"
     CDS             17376..18071
                     /gene="araD"
                     /locus_tag="RH222_001745"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV3796786.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18146..20497
                     /gene="polB"
                     /locus_tag="RH222_001746"
     CDS             18146..20497
                     /gene="polB"
                     /locus_tag="RH222_001746"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV3796787.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20662..23568
                     /gene="rapA"
                     /locus_tag="RH222_001747"
     CDS             20662..23568
                     /gene="rapA"
                     /locus_tag="RH222_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV3796788.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23580..24239
                     /gene="rluA"
                     /locus_tag="RH222_001748"
     CDS             23580..24239
                     /gene="rluA"
                     /locus_tag="RH222_001748"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV3796789.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24356..25171)
                     /gene="djlA"
                     /locus_tag="RH222_001749"
     CDS             complement(24356..25171)
                     /gene="djlA"
                     /locus_tag="RH222_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV3796790.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25426..27780
                     /gene="lptD"
                     /locus_tag="RH222_001750"
     CDS             25426..27780
                     /gene="lptD"
                     /locus_tag="RH222_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV3796791.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNSL"
     gene            27833..29119
                     /gene="surA"
                     /locus_tag="RH222_001751"
     CDS             27833..29119
                     /gene="surA"
                     /locus_tag="RH222_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV3796792.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29119..30108
                     /gene="pdxA"
                     /locus_tag="RH222_001752"
     CDS             29119..30108
                     /gene="pdxA"
                     /locus_tag="RH222_001752"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV3796793.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30105..30926
                     /gene="rsmA"
                     /locus_tag="RH222_001753"
     CDS             30105..30926
                     /gene="rsmA"
                     /locus_tag="RH222_001753"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV3796794.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30929..31306
                     /gene="apaG"
                     /locus_tag="RH222_001754"
     CDS             30929..31306
                     /gene="apaG"
                     /locus_tag="RH222_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV3796795.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31313..32155
                     /gene="apaH"
                     /locus_tag="RH222_001755"
     CDS             31313..32155
                     /gene="apaH"
                     /locus_tag="RH222_001755"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV3796796.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32233..32712)
                     /gene="folA"
                     /locus_tag="RH222_001756"
     CDS             complement(32233..32712)
                     /gene="folA"
                     /locus_tag="RH222_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV3796797.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32904..34766)
                     /gene="kefC"
                     /locus_tag="RH222_001757"
     CDS             complement(32904..34766)
                     /gene="kefC"
                     /locus_tag="RH222_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV3796798.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34759..35289)
                     /gene="kefF"
                     /locus_tag="RH222_001758"
     CDS             complement(34759..35289)
                     /gene="kefF"
                     /locus_tag="RH222_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV3796799.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35397..36728)
                     /locus_tag="RH222_001759"
     CDS             complement(35397..36728)
                     /locus_tag="RH222_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796800.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36786..37073)
                     /gene="fixX"
                     /locus_tag="RH222_001760"
     CDS             complement(36786..37073)
                     /gene="fixX"
                     /locus_tag="RH222_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV3796801.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37070..38356)
                     /locus_tag="RH222_001761"
     CDS             complement(37070..38356)
                     /locus_tag="RH222_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3796802.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38407..39348)
                     /locus_tag="RH222_001762"
     CDS             complement(38407..39348)
                     /locus_tag="RH222_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV3796803.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39363..40133)
                     /locus_tag="RH222_001763"
     CDS             complement(39363..40133)
                     /locus_tag="RH222_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV3796804.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9679 a        11101 c        10612 g         8916 t
ORIGIN      
        1 aaaaatcgca aaacatataa ttcaatacaa atcatcagga taggttttgc aacgcgtgca
       61 ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat atgtcaaccg
      121 atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca ccgcagcaca
      181 attagctaat tttacgggtg cagaactcac gctggcggga cgtttttatt gcgtcagggt
      241 tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct ggagctgatt
      301 taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct tctttgcgcg
      361 gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc
      421 cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa ggacccaaac
      481 catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac aggcgttaca
      541 ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc gtatgggtgt
      601 tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat cggtgcaaac
      661 catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg tggaaaaaga
      721 tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc atacctttat
      781 tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctggacg aggtgatcga
      841 acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg aattttcttg
      901 cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag cggcgattaa
      961 tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc cgtttgagtt
     1021 cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag ccattatttc
     1081 cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg cggtacatgc
     1141 cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc
     1201 gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg tccacaccgc
     1261 cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt gtaatatgcc
     1321 gatcccggca aacaaagcca ttgttggcag cggcgcattc gctcactcct ccggtatcca
     1381 ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag aatctattgg
     1441 tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg
     1501 catggatgag atggggtata aagaaagtga atataattta gacaatttgt acgatgcttt
     1561 cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg cgctggcctt
     1621 catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca gcgtgcagtc
     1681 tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc
     1741 agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta accgcatcac
     1801 tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc
     1861 gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg gcgtcggcct
     1921 ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga acaatatctg
     1981 gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg aaaacaacaa
     2041 ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga cggtattggt
     2101 ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg ctttgcgatg
     2161 cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca cgggcaacca
     2221 ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt tggctcggta
     2281 ggcggtccga aatgggaaca tttaccacca gaccagcaac cagaacgcgg cgcgctgttg
     2341 cctttgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact gtatcagggg
     2401 ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga catcctgtgc
     2461 gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga aggtagcgga
     2521 caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga gcgtattgcc
     2581 cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat cgacaaagct
     2641 aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc cacggaatac
     2701 ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct gattaaagat
     2761 ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct gtctgacgag
     2821 tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa cgagcaaggt
     2881 tttggtctgt atgaaccggc aggcggctca gcaccagata tcgcaggcaa aaacatcgcc
     2941 aacccgattg cgcaaattct atcgctggca ctgctgctgc gctacagcct ggatgccgat
     3001 gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg cattcgcacc
     3061 ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga tatcattgcc
     3121 cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg
     3181 ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac cgccacctgg
     3241 tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt cgcccggtac
     3301 gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag acgaaagaca
     3361 ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa aactgcaaag
     3421 aatttggcgt cgaactgtat gacctgaatc acccgtatca ggggatcgtc cacgtaatgg
     3481 ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac tcgcataccg
     3541 ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa gttgaacacg
     3601 tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac catgaaaatt gaagtccagg
     3661 gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc ggtaaaaccg
     3721 gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc cgtgatttaa
     3781 gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca aaagccggtc
     3841 tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtttgcat gcgccgaaag
     3901 gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa
     3961 ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc acctggggca
     4021 ccaatccagg ccaggtgatt tccgtgaacg acaatattcc cgatccggct tcgtttgccg
     4081 atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg aaaccgggca
     4141 ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca
     4201 ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc
     4261 aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg gaaggtctgg
     4321 ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg
     4381 cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc aaccgtaact
     4441 ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca atggctgccg
     4501 ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga gcacaccatg
     4561 gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc caatgtcgat
     4621 accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg ttttggcgcg
     4681 catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa cccggacttc
     4741 gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga aaacttcggc
     4801 tgtggctcct cgcgtgagca cgcaccgtgg gcattgaccg actacggttt taaagtggtg
     4861 attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca gctgctgccg
     4921 gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg
     4981 atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc
     5041 tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag tattgggctt
     5101 accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc gtttatgaat
     5161 taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc atcaaacgtc
     5221 tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta cccagtagac
     5281 agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca gaattacccc
     5341 ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag gcattaaatc
     5401 ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt taaaaagttg
     5461 cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa acagtactcc
     5521 tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat agtagccagc
     5581 cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac ccgccagttt
     5641 gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt acatggtgtt
     5701 gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat cctgccagcc
     5761 accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag acggaagcat
     5821 aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa acatcaccgt
     5881 aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc atgccagaga
     5941 aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt tatccgcata
     6001 ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac
     6061 accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg cattgccgat
     6121 agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt
     6181 tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa agagtccgat
     6241 ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag
     6301 cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat
     6361 acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa
     6421 aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca gtcacacatg
     6481 atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc ttcaagcatg
     6541 gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa
     6601 tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg aattattgac
     6661 ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct
     6721 gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac
     6781 acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgtaccctg
     6841 ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgcggtaaa
     6901 cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac
     6961 ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg
     7021 cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc
     7081 gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa
     7141 acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa
     7201 ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt
     7261 ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc
     7321 tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat tgtgtcgccg
     7381 acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc gttactgctg
     7441 ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa ctttgccagc
     7501 catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca actgaaaatt
     7561 caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa cgtctgggtt
     7621 ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc acagggcgag
     7681 gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt cgacagccgc
     7741 acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact
     7801 aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg
     7861 ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg cctggaaagc
     7921 ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag
     7981 cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg
     8041 catgaaggag agatcgagag cgatatctgg cttaatagcg ccaactttac gctgccgctg
     8101 gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg tattccgatc
     8161 gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc
     8221 cagcaactgg tcgccagtaa agcaatggtg ccgcttatcc accactggct gatcattcag
     8281 ggacaacgca gtatgcgcgg cctgcgtatg aataccctcg gctggttcga ttttaaatca
     8341 gcgtggtttg cgccgccgga tccatgattg ctggtagata acaaaatcac tacactaacg
     8401 ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt cgaacctgat
     8461 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt
     8521 gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt
     8581 cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact gggggcctgg
     8641 tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac tggtggcgct
     8701 ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata gtaaagccga
     8761 tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg gactatttgc
     8821 caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt
     8881 cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaaccc
     8941 gccgcaaagc ctgaaagaat tggttgagag cgatcaaaac tggcgggtga tttatcagga
     9001 tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag tctatggcga
     9061 tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg
     9121 gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga gttacaccac
     9181 ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg cgaactttag
     9241 cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga
     9301 gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg cgatcccaac
     9361 cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg aacaattgac
     9421 caaaccggcc accacgctgg agttcacacc agctgaagtg gcggcacaac gtcaggcatg
     9481 gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggtgta
     9541 agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct gtggtggaac
     9601 gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca tgtggtgcgc
     9661 ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc gatattcctc
     9721 gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca
     9781 atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt ctatggtcgt
     9841 cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt ttcgccctac
     9901 ggcctgcaag gtattttgct ggcgcacgta ttttttaatc tgccgatggc gagccgctta
     9961 ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccagcttggg
    10021 atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggttacggcg acaaatcccg
    10081 ccggttgctg cacttatctt tatgctctgt ttcgccagct tcgccaccgt gctatcactg
    10141 ggcggcggtc cgcaggcgac cactatcgag ctggctattt atcaggcgct gagttacgac
    10201 tacgatcctg cccgcgcggc gatgctggcg ctgatccaga tggtgtgttg ccttgggctg
    10261 gtactgttga gtcagcgatt gagtaaggcc attgcgccag gcaccacgct gctgcaaggc
    10321 tggcgcgacc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt aattgtgctg
    10381 gcgctgctgc tgttgctgcc accgttactg gcggtgatcg tcgatggcgt aaatcgccag
    10441 ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt
    10501 gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc
    10561 gaacttcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cggcatgttg
    10621 atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct caacaacacc
    10681 attggcctgc cgcaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcgatc
    10741 ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg
    10801 ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga gctgcgcgcc
    10861 ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat tggtgatttt
    10921 ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag
    10981 caaattggct cctatcgcag ccaggacggt gcggtcaccg cgttaattct gctactactc
    11041 tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa aactgactga
    11101 tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag aacgcggcga
    11161 gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga atttgatcgc
    11221 cggttttctg acgccagcca gcggttcgct gactatcgac ggcgttgatc acaccacgac
    11281 gccgccgtca cgccgtccgg tatcgatgct gtttcaggag aacaacctgt tcagccacct
    11341 gacggtcgcg cagaacatcg ggctggggct taatccggga ttgaagctga acgcggcgca
    11401 gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa tggcgcggtt
    11461 accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg
    11521 cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca
    11581 ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc tattgatggt
    11641 gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg
    11701 gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga gtgcttcggc
    11761 actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc cttatccgac
    11821 ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat caggcaaccc
    11881 gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat acaccggcat
    11941 caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa ttaacggcgt
    12001 cagccacaac aaacggccgc gcgacaaata atgactcaaa cggtcagtcg ctttaccact
    12061 gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg ttgccagcag
    12121 caaccattta aactcaccgc tctgcattcc ggctggaata tcgatcgccg cgcccgccag
    12181 aatccctggc aggaagtaaa acggtggcca cagcaggcag ccgataatat tcggcgtaat
    12241 gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg
    12301 gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg catgttcagt
    12361 tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg gctttttaaa
    12421 acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga tccctgccag
    12481 ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc ccgccatcag
    12541 caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg ccaccaacac
    12601 caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca atgcttgcat
    12661 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
    12721 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
    12781 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatactcgcg
    12841 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
    12901 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
    12961 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag caaacatgct
    13021 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
    13081 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
    13141 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
    13201 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
    13261 ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
    13321 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
    13381 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
    13441 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
    13501 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
    13561 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
    13621 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
    13681 attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta
    13741 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
    13801 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
    13861 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
    13921 ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc
    13981 ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag cggtgaagag
    14041 atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg
    14101 aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa
    14161 accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac
    14221 acaaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc gctgcgcccg
    14281 gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa
    14341 gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctat
    14401 attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag
    14461 gacagcgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct
    14521 ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat
    14581 aaatctctgt ggcacgaaag ctggggcggc ctgccgccag ccagtttctt tgatgagctg
    14641 gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg gactgccgat
    14701 attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg
    14761 gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg cgcacagcct
    14821 aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag
    14881 agcgttggcg agcgggcagt gaaaggtatt tgcggtcagg ttgatggcag cgtggtgcct
    14941 ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggtttggt
    15001 cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact gaaagcgcaa
    15061 atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg
    15121 tctctggatc acctgccggt ggtgctcgac tggtttaacg gccgccgcac accgaacgct
    15181 aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc tccgctgctg
    15241 ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc
    15301 gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag
    15361 gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac
    15421 cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac
    15481 atcccctcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgtgcagc
    15541 gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc
    15601 gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt tccgactcta
    15661 taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca ttggcagcca
    15721 gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgtcgttaa
    15781 tgcgctgaat acggaagcga aactgccctg caaactggtg ttgaaaccgc tgggcaccac
    15841 gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt gcgctggtct
    15901 ggtggtgtgg ctgcacacct tctccccggc caaaatgtgg atcaacggcc tgaccatgct
    15961 caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt gggacagtat
    16021 cgatatggac tttatgaacc tgaaccagac tgctcatggc ggtcgcgagt tcggcttcat
    16081 tggcgcgcgt atgcgtcagc aacatgccgt cgttaccggt cactggcagg ataaacaagc
    16141 ccatgagcgt atcggctcct ggatgcgtca ggcggtctct aaacaggata cccgtcatct
    16201 gaaagtctgc cgatttggcg ataacatgcg tgaagtggcg gtcaccgatg gtgataaagt
    16261 tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg atctggtgca
    16321 ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt acgaaagctg
    16381 ctacaccatg acgcctgcaa cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc
    16441 ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac
    16501 caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg tacagcgtct
    16561 gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaactgccg ccctgcttcg
    16621 catcatgaag gtgatgtcaa ccggtctgca gggcggtacc tcctttatgg aggactacac
    16681 ctatcacttc gagaaaggca atgacctggt gctcggctcc catatgctgg aagtctgccc
    16741 gtcgattgca gtagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa
    16801 ggacgatcct gcccgcctga tcttcaatac ccaaaccggt ccagcgattg ttgccagcct
    16861 gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg tgaaaacccc
    16921 gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc
    16981 aactgcttcc gaagcgtgga tcctcgctgg tggcgcgcac cataccgtct tcagccatgc
    17041 gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat
    17101 tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg aagtgtatta
    17161 cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt
    17221 cttatctggc ctacgcgctg tgtttttgta ggccggataa gcaaagcgca tccggcattc
    17281 aacgcctgat gcgacgctgg cgcgtcttat caggcctacg cgctgcgatt ttgtagaccg
    17341 gataagcaaa gtgcatccgg cacgaaggag tcaacatgtt agaagatctc aaacgcctgg
    17401 tattagaagc caacctggcg ctgccaaaac acaacctggt cacgctcaca tggggcaacg
    17461 tcagcgccgt tgatcgcgag cgcggcgtct ttgtgatcaa accttccggc gtcgattaca
    17521 gcgtcatgac cgctgacgat atggtcgtgg ttagcatcgc aaccggtgaa gtggttgaag
    17581 gtacgaaaaa gccctcctct gacacgccaa ctcaccggct gctctatcag gcattcccgt
    17641 ccattggcgg cattgtgcat acgcactcgc gccacgccac catctgggcg caggcgggtc
    17701 agtcgattcc agcaaccggc accacccacg ccgactattt ctacggcacc attccctgca
    17761 cccgcaaaat gaccgacgca gaaatcaacg gtgaatatga gtgggaaacc ggtaacgtca
    17821 tcgtagaaac cttcgaaaaa cagggtatcg atgcagcgca aatgcccggc gtcctggtcc
    17881 attctcacgg cccatttgca tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg
    17941 tgctggaaga ggtcgcttat atggggatat tctgccgtca gttagcgccg cagttaccgg
    18001 atatgcagca aacgctgctg gataaacact atctgcgtaa gcatggcgcg aaggcatatt
    18061 acgggcagta atgactgtat aaaaccacag ccaatcaaac gaaaccaggc tataatcaag
    18121 cctggttttt tattggattt tcaacgtggc gcaggcaggt tttatcttaa cccgacactg
    18181 gcgggatacc ccgcaaggga cagaagtctc cttctggctg gcgacggaca acgggccgtt
    18241 gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt cctgccgatc aggttccccg
    18301 cgctcagcat attttgcagg gtgaacaagg ctttcgcctg acgccgctgg cgttaaagga
    18361 ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc catcgccagt tgatgaatta
    18421 cgaaaagcgc ctgcgtgaag gtggcgttac cgtttacgag gccgatgtgc gcccgccaga
    18481 acgctatctg atggagcgtt ttatcacctc gccagtgtgg gtcgagggtg atatacgcaa
    18541 tggcgctatc gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc cgctcaagtg
    18601 ggtttctatt gatattgaaa ccacccgcca cggtgagctg tactgcatcg gcctggaagg
    18661 ttgcgggcag cgcatcgttt atatgctggg gccagagaat ggcgacgcct ccgcgctcga
    18721 ttttgaactg gaatacgtcg ccagtcgccc gcagctactg gaaaaactca acgcctggtt
    18781 tgccaactac gatcctgatg tgatcatcgg ttggaacgtg gtgcagtttg atctgcgaat
    18841 gctgcaaaaa catgccgagc gttatcgtat tccgctgcgg ctggggcgcg ataacagtga
    18901 gctggagtgg cgcgagcacg gctttaaaaa cggtgtgttt tttgctcagg ctaaaggtcg
    18961 gctaattatc gacggtatcg aggcgctgaa atccgcgttc tggaatttct cttcattctc
    19021 gctggaaact gtcgctcagg agctattagg cgaaggaaaa tctatcgata acccgtggga
    19081 tcgaatggac gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg cgacttataa
    19141 cctgaaagat tgcgagctgg tgacgcagat tttccataaa actgaaatca tgccgttttt
    19201 gctcgaacga gcaacggtga acggcctgcc ggtagaccgc cacgggggat cggtggcggc
    19261 gtttggtcat ctctattttc cccgtatgca ccgcgctggt tatgtcgcgc ctaatctcgg
    19321 cgaagtgccg ccacacgcca gccctggcgg ctacgtgatg gattcacggc cagggcttta
    19381 tgattcagtg ctggtgctgg actataaaag cctgtacccg tcgatcatcc gcacctttct
    19441 gattgatccc gtcgggctgg tggaaggcat ggcgcagcct gatccagagc acagtaccga
    19501 aggttttctc gatgcctggt tctcacgaga aaaacattgc ctgccggaga ttgtgactaa
    19561 catctggcac gggcgcgatg aagccaaacg ccagggtaac aaaccgctgt cgcaggcgct
    19621 gaaaatcatc atgaatgcct tttatggcgt gctcggcacc accgcctgcc gcttcttcga
    19681 tccgcggctg gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc aaaccaaagc
    19741 gttgattgaa gcacagggct acgacgttat ctacggcgat accgactcaa cgtttgtctg
    19801 gctgaaaggc gcacattcgg aagaagaagc ggcgaaaatc ggtcgtgcac tggtgcagca
    19861 cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg ctgaccagcg cattagaact
    19921 ggagtatgaa acccatttct gccgttttct gatgccaacc attcgcggag ccgataccgg
    19981 cagtaaaaag cgttatgccg gactgattca ggagggcgac aagcagcgga tggtgtttaa
    20041 agggctggaa accgtgcgca ccgactggac gccgctggcc cagcagtttc agcaggagct
    20101 atacctgcgt atcttccgta acgagccata tcaggaatac atccgcgaaa ccatcgacaa
    20161 actgatggcg ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc gccgtccgct
    20221 gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga
    20281 aaaccaaaag cgcggtcgcc ccttgcaata tcagaatcgc ggcaccatta agtacgtatg
    20341 gaccaccaac ggcccggagc cgctggacta ccaacgttca ccactggatt acgaacacta
    20401 tctgacccgc cagctacaac ccgtggcgga gggaatactc ccttttattg aggataattt
    20461 tgctacactt atgaccgggc aacttgggct attttgagca aaaaaaagag ttcgccagat
    20521 accattttga tgcgtgacga atgctttgcc atccagtacc atagcgccct ttccattcct
    20581 ggacctgaat aacgccacta cctcataagc acggtagcgg gtggttattg cctgcaatta
    20641 aagatataga gccgaacaca tatgcctttt acacttggtc aacgctggat cagcgataca
    20701 gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt cactttactt
    20761 ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg
    20821 atgttcaacc ctggtgatac cattaccagc catgacggct ggcagatgca agtcgaagaa
    20881 gtaaaagaag aaaatggctt gctgacctat atcggtactc gcctggatac tgaagagtcc
    20941 ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa accgcaggac
    21001 cgtctgtttg ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt
    21061 aagtattcca gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc
    21121 ctgattccgc atcagctcaa catcgctcat gatgttggtc gccgccacgc gccgcgcgtc
    21181 ctgctggctg acgaagtggg tttagggaaa accattgaag ccgggatgat cctgcatcag
    21241 caactgctct ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac cttacagcat
    21301 cagtggctgg tagaaatgct gcgccgtttc aacctgcgct ttgcgctatt tgatgatgag
    21361 cgttatgccg aagctcagca cgatgcttac aacccgtttg acaccgaaca gctggtgatt
    21421 tgctcgctgg attttgcccg tcgtagcaaa cagcgcctgg aacatctctg tgaagccgaa
    21481 tgggacctgc tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga tgcgccaagc
    21541 cgtgaatatc aggccattga acaactggca gagcacgtgc cgggcgttct gctgctgacc
    21601 gcgaccccgg aacagctggg gatggaaagc cacttcgccc gtctgcgtct gctggacccg
    21661 aaccgtttcc acgattttgc gcagttcgtt gaagagcaga aaaattatcg tccggttgcg
    21721 gacgccgttg ccatgctgct ggcaggtaac aaactgagca atgacgaact gaacatgctc
    21781 ggcgagatga tcggcgagca ggatatcgag ccgctgttgc aggcagcaaa cagcgacagc
    21841 gaagatgccc agagcgcccg tcaggagctg gtttcgatgc tgatggatcg ccacggcacc
    21901 agccgcgtgc tgttccgtaa cacgcgtaac ggtgtgaaag gattcccgaa acgcgagctg
    21961 cacaccatta aactgccgct accgacgcag tatcagacgg ctattaaagt ctccggcatt
    22021 atgggcgcac gtaaaagtgc ggaagatcgt gctcgcgata tgctctaccc ggagcgtatt
    22081 tatcaggaat ttgaaggtga taacgccacc tggtggaact tcgatccgcg cgttgagtgg
    22141 ctgatgggct acctgaccag ccatcgctct cagaaagtgc tggtgatctg cgccaaagct
    22201 gccactgcgc tgcaactgga gcaggtactg cgcgaacgtg aaggtattcg cgctgcggtg
    22261 ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg ccgcctggtt tgccgaagaa
    22321 gacaccggcg cacaggtact gctgtgctca gaaatcggtt ctgaaggacg taacttccag
    22381 ttcgccagcc acatggtgat gtttgacctg ccattcaacc cggatctact ggagcagcgt
    22441 attggtcgtc tggatcgtat cggccaggcg cacgatattc agatccatgt gccttatctg
    22501 gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga tgcatttgag
    22561 cacacctgcc cgaccggacg cactatttac gatagcgtat acaacgatct gattaactat
    22621 ctggcttcac cggatcaaac cgaaggcttt gacgatctga tcaaaaactg ccgcgagcaa
    22681 catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc gcctgctgga aatccactcc
    22741 aacggtggcg aaaaagccca ggcactggca gaaagcattg aagagcagga tgacgatacc
    22801 aacctgatcg ccttcgccat gaacctgttc gatattatcg gtatcaatca ggacgatcgc
    22861 ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga cttccctggc
    22921 ctgtcggaag atggcatcac catcaccttt gatcgtgaag tggcgctggc gcgtgaagat
    22981 gcacagttta ttacctggga gcatccgctg atccgcaacg gtctggatct gattctttct
    23041 ggcgataccg gtagcagcac gatttcactg ttaaaaaaca aagcgttgcc ggtaggtacg
    23101 ctgttggtgg aactgattta tgtggttgaa gcccaggctc cgaagcagtt gcagctcaac
    23161 cgcttcctgc caccgacgcc ggtacgtatg ctgctggata aaaacggcaa caacctggcg
    23221 gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg cggttaaccg tcacaccggc
    23281 agcaaactgg ttaacgccgt gcagcaggat gttcacgcta tccttcaact gggtgaagcg
    23341 cagatcgaga aatctgcccg tgcattgatt gatgcagcgc gtaacgaagc cgacgaaaaa
    23401 ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag tgaacccgaa cattcgtgac
    23461 gacgaactga ccgccattga gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca
    23521 ggttggcgtc tggatgccct gcgtttgatc gttgtaacgc atcagtaacg gagccgaaaa
    23581 tggggatgga aaactacaat ccaccgcagg aaccctggtt ggttatcctg tatcaggatg
    23641 accatattat ggtggtcaac aagccgagcg gtttgttgtc agtgccgggt cgtctggaag
    23701 agcacaaaga cagcgtgatg acgcgcattc agcgtgatta tccgcaggca gaatcggtgc
    23761 atcgtctgga tatggctacc agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc
    23821 gggagttaaa acgccagttc cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct
    23881 gggggcatcc atcccccgca gaaggtctgg tggatctgcc gctgatttgc gactggccaa
    23941 accgcccgaa acagaaagtc tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag
    24001 tggtggagta tgcggcggat aacacggcaa gagtggtgtt aaaaccgatt accgggcgtt
    24061 cgcatcaatt gcgtgtgcat atgctggcgc tggggcatcc gattctcggc gatcgttttt
    24121 atgcatcacc agaagcgaga gcgatggcac cgcgtttgtt gctacatgca gagatgttga
    24181 cgattaccca tccggcgtat ggcaatagta tgacgtttaa agcgccagcg gatttttaat
    24241 cgtcacgctg gcgcagttcg gtaggcggat aaggcgttca cgccgcatcc ggcaattgtg
    24301 cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg cctacagttt aaaacttatt
    24361 taaacccttt ctgctgcttt atcagctcat atgcctgctg aatttcctgc gctttctgct
    24421 tcgccatctc catcatctca ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt
    24481 cactcatcag cttacggtag gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc
    24541 ccagcacatt acaggcatct tccagcgttg ggccacgctg cgcttgctgc cagttaccac
    24601 cgccagtttg ctgctgataa ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa
    24661 actggtcaaa ctgagcgcgg gagatcccta attcttctgc aatgacatac agcaccgccc
    24721 gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg aatctggatc tccagaaaca
    24781 tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca
    24841 gcgggtaatt gtctgatttt cccacccgga acgcattttg cgccgcagta cgggaagcgc
    24901 catgaagatt cattcggtcc atcaactggc tggcgatatg aatatcagcc tccgtgacgc
    24961 gacctttgga tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct
    25021 gacgctcacg ctggttggcg aaccacgcca ttttacggct acgggcttta tcaaacatat
    25081 ggccaattaa caggcctaac actacgcccc aaaagccgcc gcccatcagt aaggccacgg
    25141 ccacgccaat gatttttccc caatactgca tatattcccc aaatcgacac acggatatca
    25201 gggctatctc ccacaatata aaggtgcttt taccgttttc cggctgcggt caattgtggg
    25261 acatcgccta taatttgcat tatcatacct gtcattcaat gccgtgccta acaccactga
    25321 cgctattcgg acaggattaa cactagcgta gagatgacga gtacgttagt ctctgaccgt
    25381 ttgtcacgcg caacgttacc gatgatggaa caataaaatc aacgtatgaa aaaacgtatc
    25441 cccactctcc tggccaccat gattgccacc gccctttata gtcaacaggg actggcagcc
    25501 gacctcgcct cacagtgcat gttgggcgtg ccaagctatg accgtcctct ggtacagggc
    25561 gataccaatg acttacccgt gactatcaat gctgaccacg cgaaagggga ctacccggat
    25621 gacgccgtgt ttactggcag cgtggatatc atgcagggta acagccgtct gcaggccgac
    25681 gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg agccggtacg taccgttgat
    25741 gcgctcggta atgtccatta cgacgataac caggtgatcc tcaaagggcc gaaaggctgg
    25801 gcgaatctga acaccaaaga taccaacgtc tgggaaggtg attaccagat ggtgggtcgc
    25861 cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg aaaaccgcta taccattctg
    25921 gataacggta gctttacctc ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc
    25981 gaaattattc atgaccgcga agaacaagtt gcggagatct ggaacgcccg ctttaaggtg
    26041 ggtccggtac cgatctttta tagcccctat ttgcagttgc cggtgggtga caaacgtcgc
    26101 tctggtttct tgatcccgaa cgccaagtac accaccacca actactttga gttctacctg
    26161 ccatattact ggaacatcgc gccaaatatg gatgccacca tcacgccgca ttatatgcat
    26221 cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc tctcccaggc gggcgctggc
    26281 ttgatggaac tggactatct gccttcagat aaagtctatg aagatgaaca cccgaacgat
    26341 gacagttcac gtcgttggtt attctactgg aaccactccg gggtcatgga tcaggtgtgg
    26401 cgtttcaacg tcgactacac caaggtcagc gatcctagct acttcaatga tttcgataac
    26461 aagtacggtt ccagtactga cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg
    26521 caaaacttca atgccaccgt ttcaaccaag cagttccagg ttttcagcga acagaacacc
    26581 agtagctact cggcagagcc gcagttagac gttaattact accagaatga tgttggtccg
    26641 tttgatacgc gtatttacgg ccaggcagtg cactttgtta acaccagaga cgacatgcct
    26701 gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc cgctctctaa taactggggc
    26761 agcatcaata ccgaagcgaa gttgctggca acccattatc agcaaactaa tcttgactgg
    26821 tataactcca gaaacacgac caagctggac gaatccgtta accgcgtaat gccgcaattc
    26881 aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc gggttatacc
    26941 caaacgctgg aaccgcgcgc gcagtatttg tacgtgccgt atcgcgatca gagcgacatc
    27001 tataactacg actcatctct gctgcaatct gactactctg gcctgttccg ggaccggact
    27061 tacggcggtc ttgaccgtat tgcctccgct aaccaggtga cgaccggtgt cacatctcgc
    27121 atatatgatg atgctgccgt tgaacgtttt aatatttccg ttggtcaaat ctactatttc
    27181 acggagtctc gcactggcga tgacaacata acatgggaga atgacgacaa aacgggctca
    27241 ctggtgtggg caggcgatac ttactggcgt atctccgagc gttggggatt gcgtggcggg
    27301 attcagtacg atacacgtct ggataacgta gcgaccagta actccagcat tgaataccgt
    27361 cgggatgaag accgtctggt acagctgaat taccgttacg ccagcccgga atatattcag
    27421 gctacgctgc ctaagtacta ttccactgct gagcaatata agaatggtat ttcgcaggta
    27481 ggtgctgtcg ccagctggcc aattgccgat cgttggtcca ttgttggggc ctactactac
    27541 gacaccaatg cgaacaagca agctgactct atgttaggtg tgcaatacag ctcctgctgc
    27601 tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt gggataacga taaacaacat
    27661 gcggtatatg acaacgcaat cggctttaac atcgaacttc gcggcctgag ctccaactac
    27721 ggtctgggta cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa ctctttgtga
    27781 tttgttgatt taccacgtaa tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa
    27841 ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat accagtttcg ctgcccccca
    27901 ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga
    27961 tggattaatg cagtctgtaa aactgaacgc tgctcaggca aggcagcaac ttcctgatga
    28021 cgcgacgctg cgccaccaaa taatggaacg tttgatcatg gatcaaatca tcctgcagat
    28081 ggggcagaaa atgggagtga aaatctccga tgagcagctg gatcaggcga ttgctaacat
    28141 cgcgaaacag aacaacatga cgctggatca gatgcgcagc cgtctggctt acgatggtct
    28201 gaactacaac acctatcgta accagatccg caaagagatg attatctctg aagtgcgtaa
    28261 caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca
    28321 ggtgggtaac caaaacgacg ccagcactga gctgaacctg agccacatcc tgatcccgct
    28381 gccggaaaac ccgacctctg atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt
    28441 cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg attgctcatt ctgccgacca
    28501 gcaggcgctg aacggcggcc agatgggctg gggccgtatt caggagttgc ccgggatctt
    28561 cgcccaggca ttaagcaccg cgaagaaagg cgacattgtg ggtccgattc gttccggcgt
    28621 tggcttccat attctgaaag ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac
    28681 cgaagttcat gctcgccata ttctgctgaa accgtcgccg atcatgactg acgaacaggc
    28741 ccgcgtgaaa ctggaacaga ttgctgctga tatcaagagt ggtaaaacga cttttgctgc
    28801 cgcagcgaaa gagttctcgc aggatccagg ttctgctaac cagggcggcg atctcggttg
    28861 ggcaacagcg gacatttttg atccggcttt ccgtgacgcc ctgacccgcc tgaacaaagg
    28921 tcaaatgagt gcaccggttc actcttcatt cggctggcat ttaatcgaac tgctggatac
    28981 ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt gcataccgca tgctgatgaa
    29041 ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt
    29101 taaaatcctg agcaactaat ggttaaaacc caacgtgttg tgatcactcc cggcgagccc
    29161 gccgggattg gcccggactt agttgtccag cttgcacagc gtgagtggcc ggtcgaactg
    29221 gttgtttgtg ccgatgccac tctccttacc gaccgggcag cgatgctcgg tttgccgctc
    29281 accctccgca cttattcccc caactctcct gcacaaccgc aaactgcggg cacattaacg
    29341 ctacttcctg tcgcgctacg tgaatctgtc actgcggggc agttagcggt tgaaaatggg
    29401 cattatgtgg tggaaacgct ggcgcgagcg tgcgatggct gtctgaacgg tgaatttgct
    29461 gcgctgatca caggccccgt gcataaaggc gtcattaacg acgcaggcat tccgtttacc
    29521 ggtcataccg agtttttcga agagcgttcg caggcgaaaa aagtggtgat gatgctggcg
    29581 accgaagaac ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga tatcgcagat
    29641 gctatcaccc ctgcgctttt gcacgaagtg attgctattt tgcatcacga tttgcggacc
    29701 aaatttggta ttgccgaacc gcgcattctg gtctgcgggc tgaatccaca cgcgggcgaa
    29761 ggcggtcata tgggtacgga agagatagac accattattc cgctgctcga cgagttgcgg
    29821 gcgcagggga tgaaacttaa cgggccgctg cctgccgata ccctgtttca gccgaaatat
    29881 ctcgataacg ccgacgccgt gctggcgatg taccacgatc agggtcttcc cgtgctaaaa
    29941 taccagggct tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat tcgcacatca
    30001 gtggaccacg gcaccgcgct tgaactggcg ggacgtggcg aagccgatgt cggcagtttt
    30061 attacggcgc ttaatctcgc catcaaaatg attgttaaca cccaatgaat aatcgagtcc
    30121 accagggcca cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga
    30181 tcgacagcat tgtttctgcc attaacccgc aaaagggcca ggcaatggtc gaaatcggcc
    30241 ccggtctggc ggcattgacc gagccggtcg gcgaacgtct ggaccagctg acggtcatcg
    30301 aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc attcttaggc ccgaaactga
    30361 cgatttatca gcaggatgcg atgaccttta actttggcga actggccgag aaaatgggtc
    30421 agccgctgcg tgttttcggc aacctgcctt ataacatctc cacgccgttg atgttccatc
    30481 tgtttagcta tactgatgcc attgccgaca tgcactttat gttgcaaaaa gaggtggtga
    30541 atcgtctggt tgcaggaccg aacagcaaag cgtatggtcg attaagcgtc atggcgcaat
    30601 actattgcaa cgtgatccca gtgctggaag taccgccgtc agcctttaca ccaccaccca
    30661 aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac gatgcctcac ccggttaaag
    30721 atgttcgtgt gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc
    30781 gtaacagcct cggcaacctg tttagcgtcg aggtgttaac gggaatgggg atcgacccgg
    30841 cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac tatctggcgg
    30901 agaacgcgcc tttgcaggag agttaacgat gatcaattcg ccccgagtgt gtattcaggt
    30961 tcaaagcgtc tacattgagg cacaatcttc acctgataat gaacgttacg tttttgctta
    31021 taccgtaacc atacgcaatc tggggcgagc gccagtgcag ttgttggggc gttactggct
    31081 gatcaccaat ggcaatggcc gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca
    31141 gccacttatc gcgcctggcg aagagtacca gtacaccagc ggtgcaatca ttgaaacccc
    31201 gctgggcacc atgcagggtc actacgaaat gatcgatgaa aatggcgtcc ctttcagcat
    31261 cgacattccc gtattccgac tcgccgttcc cacactcatt cattaaaaga atatggcgac
    31321 ataccttatt ggcgacgttc atggttgtta cgatgaactg atcgcattgc tgcataaagt
    31381 agaatttacc cctgggaaag ataccctctg gctaacgggc gatctggtcg cgcgcgggcc
    31441 gggttcgctg gatgttctgc gctatgtgaa atccttaggc gacagcgtac gtctggtgct
    31501 gggcaatcac gatctgcatc tgctggcggt atttgccggg atcagccgca ataaaccgaa
    31561 agatcgcctg acaccgctgc tggaagcgcc ggatgccgac gagctgctta actggctgcg
    31621 tcgccagcct ttgctgcaaa tcgacgaaga gaaaaagttg gtgatggccc acgcagggat
    31681 cacgccgcag tgggatctac agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct
    31741 atcgagtgac tcctatccct tctttctcga cgctatgtac ggcgatatgc caaataactg
    31801 gtcaccggaa ttgcgggggc tgggaagact gcgttttatc accaacgctt ttacccgtat
    31861 gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc aaagaatcgc cggaagaggc
    31921 ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct gtcgctgaag aatacagcat
    31981 cgcctttggt cactgggcat cgctggaggg caaaggtacg ccggaaggta tatacgcgct
    32041 ggataccggc tgctgctggg gtggtacatt aacctgcctg cgctgggaag ataaacagta
    32101 ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa gcggcggcgt cttaaacaca
    32161 gcctgatata ggaaggccgg ataagacgcg accggcgtcg catccggcac tagccgtaaa
    32221 ttctatacaa aattaccgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc
    32281 atcagcatcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa
    32341 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga tacagttttt gcgcttttgg
    32401 caagaactgt tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca
    32461 cgccgcgatg gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tacccggttg
    32521 actgctgagg ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaggtatg
    32581 gcggcccata atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
    32641 caggttccac ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat
    32701 cagactgatc attgagattt cccgataaaa aaaattgtcg ccactatacg taaagcgtaa
    32761 accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc
    32821 cacgctctct ccgtggactc gccgcattac aatgaaacaa aaacaaacag ttagctgtaa
    32881 agtgtgattt acgtcactct ttattaggat gagggtttcg tttccggttc atccgccatg
    32941 ttgccggtat gtttaccttc ttcggttccc tgccagccat gtcgttgaat taatgacaga
    33001 tgttcgcggt cctcggtaat gatctcactt aacatcgcgc tggtgcgttt atagaccgcc
    33061 gcgcgggctt tggtgtcgtt ctcaaccatt gccatctctt ccaccatctg aatattaaag
    33121 cggcggaaca catcggcacg ttctcgcgct tcatacggcc ccagacctaa actttccagt
    33181 gccagacgcc cggttttcag cgcaccttcg aaggtttcac gctccggctt ttcaacgcct
    33241 gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc gggcaataat ctgcaaatgc
    33301 gggaaatgtt ctttcaccat ctctgtcagt tgcaggttgg tttgcggatc gtcgatggcg
    33361 ttaatcagca cttccgcttt cgccgctccg gcagattcca gtaaatccat ccgcgtggca
    33421 tcgccataaa acactttcat accaaattta cgcaaggttt cgatatggtc cggatcgtga
    33481 tcgagtacca ccattttcac cccgctggag agcagtaaac gtccggtaat ctgcccaaaa
    33541 cgaccgaatc cggcgataat cacgcgcggc tgttcttcgt cgatctcatc ggcttcacgc
    33601 gcttcctcag tagaagattg ctcaaggcga ttgaggatca ccagcagaat cggcgttgct
    33661 gccatcgaca gcgccaccgc cagggtcagc gatttcgccc actccggctc cagcacattc
    33721 gccatctgcg ccgcgccaaa taccacaaag gcaaactcac tgccctgccc taacaacacc
    33781 gcaaaccaac gacgctgttt atttggcact tgcaacggtc gggcaatcag ccacagcatg
    33841 gcgattttga tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg gttttcaagc
    33901 agcgtgccaa agtctatcga catgccaaca ccgatgaaaa acagccccaa cagcaaacct
    33961 ttaaatggtt cgatatcgct ctccagcgca tgacggtatt ccgagcttgc cagcagtacg
    34021 cccgccagaa acgcgcccat cgccatcgac aagccgacct cttccagcag caaaccaaag
    34081 ccaaacacga ggaataacgc cacggcacta aacacttccc gcaagccaga gcgggctaca
    34141 aaacgcagcg ccggacgcgt gacatagcgc cccagcaata ccaccagcac cagcgcaccc
    34201 gccactttta acgccgagag agcaaatgcg cccatcgtcg tcgaggcact gctcgttgcc
    34261 agtagcggaa tcatcgccac cagcgggatc gccgcgatat cctggaacag cagcaccgca
    34321 aaggcactgc gacccatttg cgtcaccatc agattgcgtt cattcatcgc ctgcatggca
    34381 atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc
    34441 ccaagtaaca tgcagaacag ccccagcagg ccaccgcaaa tcaccatctg caatgcgcca
    34501 ccgccgaaca ctgccgcacg cagcttccac agcctttgtg gatcgagttc gaggccgata
    34561 ataaacagca tcagcaccac gccaatctcg gcaaagtgca gaatagattc ggcatcggtc
    34621 accagtcgca gcccccacgg gccaataatg cagccggcga tcaggtagcc aagtaccgat
    34681 cccagaccaa gacgtaccgc aatgggtaca atcagcgctg ccgaaccgag ataaatcagc
    34741 gcctgaatca gcgtatggct atccatgatg ggcctcctgc cattccagca gacgttgctt
    34801 atagtgacgc gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc
    34861 aaacggtggc agccagttca gcccgcagta gattgccgtc gcctgtagcg gctgcgacag
    34921 cacatcaaag cccggatgcg caccaatttc aaaatggctt tccccgccgc cggtcgtcac
    34981 cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg tgaccgtaag cccagccgtg
    35041 cgagaaaact ttatcgatcc aaagtttgag gagcggtgga atgctgtacc actgcatcgg
    35101 gtgctgccag acgatcagat cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat
    35161 attgaagtca ggatagagtt gataaagaga gcgaatttcg acgccttcca gcgtccttgc
    35221 ctgttcaagc atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaattat
    35281 aagaatcata ggtagcccgc gtgattacgc ctttgttttc ccacaaagtg tagtcatata
    35341 ttcacgcggc ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaaattac
    35401 cccattctct gcccgcggat cgtcatattg ctggtctgcg ccagtgacat ccctcgagtc
    35461 tccggggcaa acgctacgga aatcaacaag ccaaacagcg agatacccgc ccccattagc
    35521 atcgtgttac tgataccgta attattgata aagatcggca gtgcccaggt cgaaacaatg
    35581 gtgccaatac ggcttaagga cataatcacg cccacggcag aggcgcggat atctgtcggg
    35641 aagagttcat taggatagag ccactgcaaa ttacccggcc cgccagagaa aaaggcatac
    35701 accgcaaagg ccatcactac cagccagatc cccatatccg ggatcagccc caaaaccgcc
    35761 agcgccagcg tcatcatggc aaagctgcca atcaacaatg gacgccgtcc ggcagtgttt
    35821 aaccacagca tcggcggaat acagccgagc ataaagaaca ggctaatcac cacgttcccc
    35881 agtgccgcgt ttttgccaac ccccaatccc aacaaaccaa cgatttgcgg gccaaaggtg
    35941 taaatggcga acattgggat cacctggcag gtccagatgg cggcaacaaa cagaacaaaa
    36001 ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt gctgcggctg ctcttcatcg
    36061 aaagccaccg gttcgccaaa cagttttatc atcatctctt cgcactcttt tactcgccct
    36121 ttgcgtaata accagcgggg agattcaggc agttcgaatc gaccaatcaa aatcaacaaa
    36181 caggggatcg ccgcgctacc cagcatccag cgccagccgc cttccacatc ataaagccag
    36241 tagccgacca gatcggcgca ggtcgcgccg acataccaca tggcggcgat aaagctgatg
    36301 gaaaacgccc gctgacgggt actggagaac tcggtgatca ttgaggtggc gatgggataa
    36361 tctgcaccga tgacaatgcc gataagtacc cgcatcacca acagttcgac aggggatgag
    36421 acaaacatcg tcgccaccga tatcacgccg atggcgatga tatcaatgag gaacattttg
    36481 cgccgtccga ctttatcgga gatatagcca aacagcgatg tgccaacgaa cagcccggcg
    36541 agcgttcccg cgcccagcaa gccaatccag tcagcgtcca gtttcagcgc cggagtcagt
    36601 tgctccagcg ccacgccaat cattaccagt acataaccat ccagaaacgg accaccgctt
    36661 ccccacagca aaatgcggcg gtgaatagag gagaatttga gatcgtcaaa gtttctggac
    36721 ggttgcatgg gttattcctg ttttttgcaa tgagagtcag tgcggcgtta cgtatcaaac
    36781 caacatcagc cgtaacggaa ctccacacca aaggtgccgc gcgggtattc ccactgttcc
    36841 agcgccgacc ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg
    36901 aagcgcacac tgccgtcatc ctgcttcttg tacagacctg cggggcacgc tttcaccagc
    36961 agctccagca cctgtttatc agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg
    37021 acattgaatt tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg
    37081 tcactccttt catgccatcc ttgattagat tgatgaagcc cactttctta ccgtggcgga
    37141 ggattttctt gcgcatcagt tctggcgcac tgccgtcaat ggtgaacagg tcacgcgcca
    37201 cgcccaccgc cagctccggg tagccgctga acatgcgtgg gttatcaagg aacgccggta
    37261 gtttctggta catgcgcata tcgcgcagcg ggccactctc aagatgctga cgatattccg
    37321 ccagtttttg cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag
    37381 cttccccggc ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca
    37441 ttccggcggc atcgccggca atcaatacgc cgtcaccgac cagctccggc agcatgttga
    37501 tgcctgcttc cggcactacg tgagcggaat attccaccag cttgccaccc gcgatcagcg
    37561 gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg cggcaccgat ttttttgcgt
    37621 catgcagatg atgcagacca caaaccagcc ccagcgacag ggtattttca ttggtataaa
    37681 ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct
    37741 gattaccctg caactgaaaa cggtcttcga ttaccgactt cggtaactcg atcagttcct
    37801 tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc catccccagc ttttcggcaa
    37861 ggatggagtt cactccatca gcaaggatca ccgttttcgc ttcaatcaca tcgccatcgg
    37921 cttctacacc gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa
    37981 ttaactgcgc gcccgcttct tcggcctgct ccatcagcca ggcatcaaat ttactgcgca
    38041 aaacggagta agaacgctgg gatggcgagg tttcgtcacc attgcagtag tccatagtca
    38101 tcgctgactt ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctacggggg
    38161 cggagtcggc gaaaccagga ataatgtgtt ccaggctgtg ggcatagaga cgcccgccgg
    38221 tgacgttctt ggcacctgcg gaattgccac gctcgataac taacacttgc gccccttcgc
    38281 gggcgagcac cagtgcggca accgaaccgg caagcccagc accgacgatg atggcgtcaa
    38341 agatatcttc ggacataaga gctccctgtc agcggcaggg ccaaaatgca gccctgccag
    38401 agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg atcttcacgg cgtcaccaac
    38461 aatgccgtaa tccgcgtact ggaagatcgg cgcattttta tctttattga tggcgaaaat
    38521 ggtttgcgat gcattagcgc caaccatgtg ctggatctgc ccggagatcc ccaccgccag
    38581 gtacagttca ggtttcagca tcaggttgga gataccgaca tagcgttcgt gctccatcca
    38641 tttttcgttt tccgccaccg gacgagaaca ggccaactcc gcacctatcg ccttgcaaag
    38701 ctgttctgcc agcgcaatgt tctctttgct gccaataccg cgaccgacgc tgaccaccag
    38761 acgggctttg tcgagatcga cgctgttgct ctggcgcgcc tgggttgccg tgcgggtaat
    38821 cgccacagcc ggagcctgcc actccacggt gtgcgtttcg ccagtgcgtg acgcgtctgg
    38881 ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc gcatacggcg tggcaatgcg
    38941 ttcttcgcca atcgccagac caccgtaaac catgtgtttc actgtcgctt taccgtcctg
    39001 tacgctgacg gtgctggcat cgttagacac cgccgcttta aggcgataac ccagttttgc
    39061 cgccagtaat ttgccgcgac gggtgtttgg cagcagcacc aggccgtctg cgccgtgctg
    39121 gcgaatagtg tcagccatga caccggcgta atcttcgatc atccgatcgt ccggtttgcc
    39181 gtttaatttc cagacatgat tagcgccgag ctggattgcc tgtgcgccgt cggcatcatt
    39241 gaggacaaag gtgttgattt gattagctaa agcctgcgca ccgttcatca gttccggcag
    39301 acgagaaggg gtatcgctga atacccagac ttgagaaaac gtgttcatag catcccctgt
    39361 aattaaatga ctttgcgaag attttcagca aatgcggcga tctgttcttc gccgtcgcct
    39421 tcaatcacga tgcgctgacg ttcgcgctgt ttcggcgcgg caacctgttg ttctgaccag
    39481 gctgcctctg cgttaaaacc aatatccgcc gccgaccata cctggacggg ctttttcgcc
    39541 gcgccgagaa tggctttcat cgaaggaatt tgtggggaat tgatatcagt ggaaacagca
    39601 acaaccgcag gcagcggaat gcttaaggtt tcggtttcat cttccagttc gcgctcaacg
    39661 gtgagggtat ctgcagtcag ggagataatt ttgctgacgc cgttaactgc cggaatattg
    39721 aggatttcgc ccaccagcag accaacctgc tgggcataaa ggtcggaaga accatcgcca
    39781 cagaggatca aatcaaagcc tgctttctgg gcggctgcag ccagtgcgct cgccgtttgt
    39841 tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa tcagttcatc cgggccgcgc
    39901 gatagcacat ctttacgccc tttggcgttg gtcagggctt taccgcccac acttaaggcc
    39961 gtcacctgcg cctctgctgc ctgttgcttt agctggcaag ccgcttcaat agcgttcaga
    40021 tcgtattggc ttattttggc atcggctttg ctgaagtcta atgaaccatc agcattattg
    40081 accgcaatat cctgttcatc aggcacgcac ttatagcaag taataatctt cattgcatct
    40141 ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt taattacaga tatcacgccc
    40201 tttaatttgg aaataaaact ttaatcacca atattgaaaa tgtcacacgc agataaatcc
    40261 aactttcaat attgttaagt tcctcaccaa tattgaaaac atggcgta
//
LOCUS       DAPHJE010000034        39509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPHJE010000034 DAPHJE010000000
VERSION     DAPHJE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.034"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(104..799)
                     /gene="aqpZ"
                     /locus_tag="RH222_001764"
     CDS             complement(104..799)
                     /gene="aqpZ"
                     /locus_tag="RH222_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV3796805.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1225..2883
                     /locus_tag="RH222_001765"
     CDS             1225..2883
                     /locus_tag="RH222_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV3796806.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2880..3836)
                     /locus_tag="RH222_001766"
     CDS             complement(2880..3836)
                     /locus_tag="RH222_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDV3796807.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3987..5102
                     /gene="macA"
                     /locus_tag="RH222_001767"
     CDS             3987..5102
                     /gene="macA"
                     /locus_tag="RH222_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV3796808.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5099..7045
                     /gene="macB"
                     /locus_tag="RH222_001768"
     CDS             5099..7045
                     /gene="macB"
                     /locus_tag="RH222_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV3796809.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7118..7342)
                     /gene="cspD"
                     /locus_tag="RH222_001769"
     CDS             complement(7118..7342)
                     /gene="cspD"
                     /locus_tag="RH222_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV3796810.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7665..7985
                     /gene="clpS"
                     /locus_tag="RH222_001770"
     CDS             7665..7985
                     /gene="clpS"
                     /locus_tag="RH222_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV3796811.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8016..10292
                     /gene="clpA"
                     /locus_tag="RH222_001771"
     CDS             8016..10292
                     /gene="clpA"
                     /locus_tag="RH222_001771"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV3796812.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10636..10723)
                     /locus_tag="RH222_001772"
     tRNA            complement(10636..10723)
                     /locus_tag="RH222_001772"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10687..10689),aa:Ser,seq:gga)
     gene            complement(10977..11195)
                     /gene="infA"
                     /locus_tag="RH222_001773"
     CDS             complement(10977..11195)
                     /gene="infA"
                     /locus_tag="RH222_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV3796813.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11480..12184)
                     /gene="aat"
                     /locus_tag="RH222_001774"
     CDS             complement(11480..12184)
                     /gene="aat"
                     /locus_tag="RH222_001774"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV3796814.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12226..13947)
                     /gene="cydC"
                     /locus_tag="RH222_001775"
     CDS             complement(12226..13947)
                     /gene="cydC"
                     /locus_tag="RH222_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV3796815.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13948..15714)
                     /gene="cydD"
                     /locus_tag="RH222_001776"
     CDS             complement(13948..15714)
                     /gene="cydD"
                     /locus_tag="RH222_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV3796816.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15837..16802)
                     /gene="trxB"
                     /locus_tag="RH222_001777"
     CDS             complement(15837..16802)
                     /gene="trxB"
                     /locus_tag="RH222_001777"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV3796817.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17346..17840
                     /gene="lrp"
                     /locus_tag="RH222_001778"
     CDS             17346..17840
                     /gene="lrp"
                     /locus_tag="RH222_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV3796818.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17975..22081
                     /gene="ftsK"
                     /locus_tag="RH222_001779"
     CDS             17975..22081
                     /gene="ftsK"
                     /locus_tag="RH222_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV3796819.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22236..22847
                     /gene="lolA"
                     /locus_tag="RH222_001780"
     CDS             22236..22847
                     /gene="lolA"
                     /locus_tag="RH222_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV3796820.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22858..24201
                     /gene="rarA"
                     /locus_tag="RH222_001781"
                     /gene_synonym="mgsA"
     CDS             22858..24201
                     /gene="rarA"
                     /locus_tag="RH222_001781"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV3796821.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24292..25584
                     /gene="serS"
                     /locus_tag="RH222_001782"
     CDS             24292..25584
                     /gene="serS"
                     /locus_tag="RH222_001782"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV3796822.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25823..28267
                     /gene="dmsA"
                     /locus_tag="RH222_001783"
     CDS             25823..28267
                     /gene="dmsA"
                     /locus_tag="RH222_001783"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV3796823.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNTGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28278..28895
                     /gene="dmsB"
                     /locus_tag="RH222_001784"
     CDS             28278..28895
                     /gene="dmsB"
                     /locus_tag="RH222_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV3796824.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28897..29760
                     /gene="dmsC"
                     /locus_tag="RH222_001785"
     CDS             28897..29760
                     /gene="dmsC"
                     /locus_tag="RH222_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV3796825.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29796..30422)
                     /locus_tag="RH222_001786"
     CDS             complement(29796..30422)
                     /locus_tag="RH222_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3796826.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30736..31884
                     /locus_tag="RH222_001787"
     CDS             30736..31884
                     /locus_tag="RH222_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3796827.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32094..33524
                     /locus_tag="RH222_001788"
     CDS             32094..33524
                     /locus_tag="RH222_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3796828.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGRGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33525..34433)
                     /locus_tag="RH222_001789"
     CDS             complement(33525..34433)
                     /locus_tag="RH222_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3796829.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34533..35123
                     /locus_tag="RH222_001790"
     CDS             34533..35123
                     /locus_tag="RH222_001790"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDV3796830.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(35205..35945)
                     /gene="pflA"
                     /locus_tag="RH222_001791"
     CDS             complement(35205..35945)
                     /gene="pflA"
                     /locus_tag="RH222_001791"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV3796831.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(36137..38419)
                     /gene="pflB"
                     /locus_tag="RH222_001792"
     CDS             complement(36137..38419)
                     /gene="pflB"
                     /locus_tag="RH222_001792"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV3796832.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38474..39331)
                     /gene="focA"
                     /locus_tag="RH222_001793"
     CDS             complement(38474..39331)
                     /gene="focA"
                     /locus_tag="RH222_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV3796833.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9841 a         9639 c        10435 g         9594 t
ORIGIN      
        1 aatatgtggc ttagctcatg aaaggagcgc aacaagatga tgaatcaaca tataacaaca
       61 tcttaaaaaa aggcctgaca ttacgccagg ccttctgcgt taattaatca cgcttttcca
      121 gcagggtccg gtaaatcaga ccaccgataa tgccgccgac aattggcacc acccagaaga
      181 accacagttg ttctaatgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg
      241 ggttaacaga agtgttagtc accggaatac taattaagtg aatcagggtt aaggccagac
      301 caatagcgat cggcgcaaaa cctgccggcg cgaatttgtc ggttgtgccg tggatcacca
      361 acaggaaacc tgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc
      421 ctggtgaatg ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg
      481 ttttaccact ggcaattaaa tacagcagcg ccgctgcaac aataccgccg acaacctggg
      541 caattacgta gccaacgact tcttttgccg gaaaacgtcc gccagcccat aaaccaatag
      601 tgaccgccgg gttaaaatga ccaccagaaa tatgaccaac agcaaaggcc atcgtcagaa
      661 cggtcagacc gaacgccaac gccacgccgg caaaaccaat gcctaattcc gggaagcctg
      721 cggccagtac agcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag
      781 ctgctaattt tctgaacata tccacctcaa ttaaaaattg accctgtgaa aaatatggtc
      841 gttttatagg gtcgtcgtaa aaaagtgacg acggaaataa tgcgcggcta ttttaaaaac
      901 gaaggcgagt cattcaccag ataaataaat ccagtaaatt tgatttaggg caacagcggg
      961 ttgccccata tagtcatttg tctgattgac agtgtagtgc aagcaaaaga tttaatcctt
     1021 taggcgtaat aaaaaataat ttatcatgct aattatttga ttttgttgtt tttgcagact
     1081 tatcagcaag agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt
     1141 aaatgtgatg tcatcacgta ttagcaaggc ctttcccgtt atactgccag cgtaaaggat
     1201 aagtcacata tttctggagg ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc
     1261 ggtatcaacc gtttgtcgtt gatgctcgaa caaaacaacg tcctgattgg ggagaacgcg
     1321 tggggtaaat ccagcttgct ggacgcctta accctgctgc tatcgccaga atcagatctc
     1381 taccattttg agcgcgacga tttctggttc ccgccgggag atatcaacgg gcgagaacat
     1441 catctgcata ttattttgac cttccgcgaa tcgctgccag gccgacatcg ggttcgccgt
     1501 tatcggccgc tggaagcgtg ctggacgcca tgcaccgatg gctatcaccg tattttttat
     1561 cgtctggaag gggagagtgc ggaagacggc agcgtgatga cactgcgcag ttttctcgat
     1621 aaagacggac atccgattga tgtcgaggat attaacgatc aggcacgcca tctggtgcgt
     1681 ttaatgccgg tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg taacggcacg
     1741 gtgccaaatg ttcccaatgt ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag
     1801 ttgtcctcac atccgcaaaa tctctctgat ggacagattc gtcagggact ttccgcaatg
     1861 gtgcagttgc ttgagcatta tttctctgag cagggggccg gacaggcgcg atatcgttta
     1921 atgcggcggc gggccagcaa tgagcaacga agctggcgct atctggacat catcaaccgg
     1981 atgattgacc gacctggagg gcgctcgtat cgggttattt tgctcggcct gtttgctact
     2041 ttattgcagg caaaaggcac attgagactg gataaagacg cgcgtccatt gttgctgatc
     2101 gaagatccag aaacccgttt acaccccatt atgctttcag ttgcctggca tctgttgaat
     2161 cttctgccat tgcagcgcat tgccaccacc aactcgggtg agttgctttc gttaacgccg
     2221 gtagagcatg tttgccgact ggtacgtgag tcctcgcgcg ttgccgcctg gcgtctgggg
     2281 ccgagtggct tgagtaccga agatagccga cgcatatcct ttcacattcg ttttaaccgt
     2341 ccgtcatcgc tgtttgctcg ctgctggttg ctggtggaag gggaaacgga aacctgggtt
     2401 atcaatgaac tggcgcgcca gtgtggacac catttcgatg ccgaagggat caaggtcatt
     2461 gagtttgccc agtccgggct aaagccactg gttaaatttg cccgccgaat ggggattgaa
     2521 tggcatgtac tggtcgatgg cgatgaagca gggaagaaat atgccgctac ggtacgcagc
     2581 ctgttgaata acgatcggga agccgaacga gaacatttaa cggcgttacc ggcgctggat
     2641 atggaacatt ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc
     2701 ccggaaaatg taccgatgaa tctgcgcaaa attatctcga aagcgatcca tcgctcttcc
     2761 aaacccgatc ttgccattga agtggcaatg gaggccggac gtcgtggtgt ggactccgta
     2821 ccgacgctgc tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat
     2881 taaccgcgaa acatcgtggc catttgtggc tgaatagcgt cgagcatctc atagcgccga
     2941 cggtattcag cccgtttttt actggcgatt tcggcaatct cttttcgtgc tatctgtgct
     3001 ggaaggcggt aatggcgttc agcatcacat acgccgccaa ccgattccca gaaagcgttg
     3061 taatcggcat ggatcttgcc ttctttatca cgataacgca ggctgcggta aatatgcgtt
     3121 tcattgctga cggcaataat ctgctctacc tgcaaacgtt gggcaaacag acaggccgct
     3181 tccatcacga ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc
     3241 tgatgtggaa tttcccattt tgcgccttgc agtccgccaa taaacatcgt tcttttcccc
     3301 tgatattcac acagggtaaa cgtgatctct gccagaggaa taccttcgct gttgcgaaac
     3361 aggattgtgc tgtcaccttc tttatccatt gagatcatca tggtcagctc aagcgtgaac
     3421 tgctcgccgt ttttgccttc cagtttagcc agttgcagcc cgggggtatt caaatataag
     3481 ctgaattctt ccgccgacat acatccgcgg agtaacgcat aatggtaacg taacgcctcc
     3541 agcaattgct tacggctaag attcgccgca aggtaagggc gatgcagacg cacaggcagt
     3601 cgcggttggc gcgttaacaa cacattgaga ttaggccagt gggaaagttc gttcatccac
     3661 tcaacgctta aacgcggcat aatcaacgag cgcagcaaaa atttctggcg aaaactacgg
     3721 cgatgccaga atttacccgg ccgacactgt ccacgtgcca gactaagaaa aagtgacagg
     3781 ctgctgagag attcagatgg cgtaaaggtc cgttcagtta gctgcgacat attcatgaaa
     3841 tcaatggtta tacatgacgt cgatttcacc attgcgtatc ttaaccaaac atcaatagtg
     3901 tgattactaa cgtaaatttt agggttttgt tgatatttcg ttgaagttaa tgacccggat
     3961 tggcatatgg agtattcaga atatttatga aaaagcggaa aaccgtgaag aagcgttacg
     4021 ttattgcgct ggtgatagtc atcgccggac tgattacgtt atggagaatt cttaacgcac
     4081 ccgtgccgac ttatcagaca ctgattgtgc gccccggtga tttacagcaa agcgtgctgg
     4141 cgaccggaaa gctggacgcg ctgcgtaagg ttgacgtggg cgcgcaggtc agcggtcagt
     4201 tgaaaactct gtcggtggcg attggcgata aagtaaaaaa agaccagctt ttaggggtta
     4261 ttgatcctga acaggctgaa aaccagatta aggaggtcga agcaacgctg atggagctac
     4321 gtgcgcagcg gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg tattcccgtc
     4381 agcaacgtct ggcacaaacg caggcggttt cacagcagga gctcgacacc gccgcgacgg
     4441 agatggctgt gaaacaggcg caaattggca ccattgacgc gcaaatcaag cgcaatcagg
     4501 cctctctcga tacggctaaa accaatctcg attacactcg catcgttgcc ccgatggccg
     4561 gggaagtcac gcaaatcacc actctgcaag gccagacggt gattgccgca caacaagcac
     4621 cgaacattct gacgctggca gatatgagca ccatgctggt aaaagcgcag gtttctgaag
     4681 cggatgtaat ccacctgaag ccggggcaaa aagcctggtt tacggtactt ggcgatccac
     4741 tgacgcgcta cgaggggcaa atcaaggatg tactaccgac gccggaaaag gttaacgacg
     4801 ctattttcta ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg cggctggata
     4861 tgactgcgca agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt
     4921 cggcgttagg cgatccggtt ggcgataatc gttataaagt caaattgttg cgtaatggtg
     4981 aaacacgcga gcgtgaagtg acgattggcg cacgtaacga taccgatgtt gagattgtca
     5041 aagggcttga agcgggcgat gaagtggtga ttggtgaggc caaaccagga gctgcacaat
     5101 gacgcctttg ctcgaattaa aggatattcg tcgcagctat cctgccggtg atgagcaggt
     5161 tgaggtgctg aagggcatca ccctcgatat ttatgccggt gagatggtag cgattgttgg
     5221 cgcttcaggt tccggtaaat cgaccctgat gaatattctc ggctgtctgg ataaggccac
     5281 cagcggcacc tatcgcgtcg ccggtcagga tgttgccaca ctggacgccg atgcgctggc
     5341 gcaactgcgc cgcgagcatt tcggctttat tttccagcgt taccatttac tttcgcattt
     5401 aaccgccgag cagaacgttg aagtacccgc cgtctatgct ggtcttgagc ggaaacagcg
     5461 actgcttcgt gcccaggagt tgctgcaacg gctggggctg gaagaccgta cagagtatta
     5521 tccggcacag ctttcgggtg gtcagcaaca gcgcgtcagc atcgcgcggg cattgatgaa
     5581 cggtggtcag gtaattcttg ccgatgaacc aaccggcgca ctggacagcc attctggcga
     5641 agaggtgatg gcgatcctgc atcagctgcg cgatcgtggg catacggtga ttatcgtcac
     5701 ccacgatccg caggtcgctg ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat
     5761 tgtgcgcaat cctcccgcca ttgaaaaagt gaatgttgct ggcgggacgg agcctgttgt
     5821 caacacggtg tctggctggc ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc
     5881 atggcgggcg ctggcagcga ataaaatgcg tactttactg accatgctgg ggattattat
     5941 cggtattgcg tcggtggttt ccattgtcgt ggtgggtgac gccgccaaac aaatggtgct
     6001 ggcggatatt cgttctattg gtacgaatac tattgatgtc tatcccggga aagattttgg
     6061 cgatgacgat ccgcaatatc agcaggcgct gaagtacgac gacttaatcg ccatccaaaa
     6121 acaaccgtgg gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa
     6181 caatgttgat gttgctgcca gtgccaatgg cgtgagcggc gattatttta atgtctatgg
     6241 catgaccttc agtgaaggaa acacctttaa tcaggagcag ctgaacggtc gtgcgcaggt
     6301 cgtggttctc gacagtaata ctcgccgcca gcttttcccc cataaagcag atgtggttgg
     6361 cgaggtgatt ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca
     6421 gtcgatgttt ggtagcagta aagtgctgcg tgtctggcta ccttacagca cgatgtccgg
     6481 gcgagttatg ggccagtcgt ggctgaactc cattactgtc agggtaaaag aaggatttga
     6541 cagcgccgag gcggaacagc aactcacgcg tttactttca ctgcgccacg gaaagaagga
     6601 tttctttacc tggaacatgg acggcgtctt gaaaactgtt gaaaagacca cacgtacttt
     6661 acaactgttt ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat
     6721 gaatattatg ctggtgtcag tgaccgagcg gacgcgggaa attggcattc gcatggctgt
     6781 aggtgcgcga gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac tggtttgcct
     6841 ggtcggtggc gcgttgggaa taacactgtc actgttaatt gctttcacct tgcagctttt
     6901 cttacccggc tgggagattg gtttttcacc gttggcactg ctgctggcgt ttctctgctc
     6961 gacggtcacc gggattttat ttggatggtt acccgcacga aatgcggcac gactggatcc
     7021 agtagatgct ctggcacgag agtaattttt gagataaaaa tgccagccga tcgggctggc
     7081 attttgcctt taggatgtac acaatgagac agaagagcta tgcgactgcc gcttctactt
     7141 cgacgggcac aataacactg gcgtgattgc cttttggccc ctggtggaca tcaaactgaa
     7201 cggattgtcc agcttttagc gttctgtaac catccatctg aatggtggaa taatgagcga
     7261 aaatatcttc gccgccgcct tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc
     7321 acttaacagt acccttttcc atgcttcgac atccttcgca aatcttatac aagtaagatg
     7381 gaataaaccg gggtcagaga gggggctgtt caaaacctcg ccaactctag aaatacaatt
     7441 tagagaatta gggcgagccg tcaagcgttt gacaggggcc agggggcagg tatgaatcaa
     7501 aaatttgaag cagttaacgc tattgacagg aatgtgacag atgtcgctga tgccaacgat
     7561 agatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg
     7621 cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt aaaacgaacg
     7681 actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat
     7741 ctatgtataa agtgatatta gtcaatgatg attacactcc gatggagttt gttattgacg
     7801 tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc
     7861 actaccaggg gaaggccatt tgcggagtct ttaccgccga ggttgcagaa accaaagtgg
     7921 cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg ctagaaaaag
     7981 cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa ctggaactca
     8041 gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg accgtcgagc
     8101 acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa gcgtgttctg
     8161 tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc acacccgttc
     8221 tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag cgtgtactgc
     8281 aacgtgcggt cttccatgtc cagtcctccg gtcgcaatga ggtaaccggt gcaaacgttc
     8341 tggtcgctat ctttagcgaa caggagtcgc aggcggcata tctgttgcgt aaacatgaag
     8401 tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac gagccgacac
     8461 agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg gaggaacgta
     8521 tggagaattt cacgacgaac ctgaatcagc ttgcgcgcgt gggcggaatc gacccactga
     8581 ttggtcgtga gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca
     8641 acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc gattgcggaa ggtcttgcct
     8701 ggcgaattgt tcagggcgat gtgccggaag tgatggctga ctgtacgatt tactctctcg
     8761 atatcggttc tctgttagcg ggcactaaat atcgcggcga ctttgaaaaa cgttttaaag
     8821 cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat gagatccaca
     8881 ccattatcgg tgcgggtgca gcgtctggtg gccaggtcga tgcggctaac ctgatcaaac
     8941 cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag gagttcagca
     9001 acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat attactgaac
     9061 cgtcgatcga agaaactgtt caaatcatca atggcctgaa accgaagtat gaagcgcacc
     9121 acgacgtgcg ttataccgca aaagcggtgc gtgcagcggt agagctggcg gtgaaataca
     9181 ttaacgatcg tcatctgccg gataaagcca ttgacgttat cgacgaagcg ggcgctcgcg
     9241 cacgcctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat attgagtccg
     9301 tggtggcccg tattgcgcgc attccagaga agagtgtttc tcagagtgac cgcgataccc
     9361 tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa gccattgagg
     9421 cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat aaaccggttg
     9481 gttcgttcct gtttgccggc cctaccgggg tcgggaaaac agaggtgacg gtacagcttt
     9541 cgaaagcgtt gggcattgag ctgctgcgct ttgatatgtc cgagtatatg gaacgccata
     9601 ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag ggcggtttgc
     9661 tgactgatgc ggtcatcaag catccacatg cggtgctgtt gctggacgaa atcgagaaag
     9721 cgcatccgga cgtgttcaat attctgttgc aggtgatgga caacggtacg ctgaccgata
     9781 acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccacc aacgccgggg
     9841 tacgtgaaac tgagcgtaaa tccattggtc ttatccacca ggataacagc accgatgcga
     9901 tggaggagat caagaagatc tttacaccgg aattccgtaa ccgtctcgac aacattatct
     9961 ggtttgatca tctgtcaacc gacgtgatcc atcaggtggt ggataaattc atcgtcgagt
    10021 tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact
    10081 ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg cgtgtcatcc
    10141 aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg gtggacggcg
    10201 gtcaggtcac cgtcgcgctg gataaagaga aaaatgagct gacttacgga ttccagagtg
    10261 cacaaaagca caaggcggaa gcagcgcatt aatctgattg ttaggtaggt tggtcaagtc
    10321 cgtaatctcg aaagaggtta cggacttttt gtttatgggg tggaggaggt tcagaccctt
    10381 tttttaatga tgatggtaag ttgttgataa ttagtgctgc gggaaggtaa ggataaaaaa
    10441 gggtgctgca ggagaatggg atggttttgc tttattaaca acgggctaaa cgtgtagtat
    10501 ttgagttcac tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga
    10561 acgcgttcac tgccgtacag acagataaaa tgcgaaaaaa aagctcgcac tttcgtacga
    10621 gctcttcttt aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac
    10681 acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca
    10741 aacctcatgg gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga
    10801 attttaatga taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc
    10861 gagacaagtt acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc
    10921 cggttaaacc gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag
    10981 cgactacgga agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact
    11041 ttgtcgcccg tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta
    11101 accacgtgac cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg
    11161 gtaccttgca tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg
    11221 taatttgaac cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa
    11281 atcgcaacaa acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag
    11341 cgcaacgtat aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaaatcct
    11401 tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg
    11461 agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa gcatcgtggt acccagaaat
    11521 tattcggcaa tcgtccgagg cgcatttgat tgagataatt aaggtaatcc cggcggggaa
    11581 tttcgcaggc accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac
    11641 cgccatgacc gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca
    11701 tccggctgaa catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc
    11761 cgacaagctc atcttcacgc cagacttcaa tggagtgggc atggccgagt tcgtgaaggc
    11821 gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac
    11881 agccttcaat gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat
    11941 gaaatcgctt catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat
    12001 cgggcgacca ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt
    12061 aagccattaa caggcgcgca gggctaagat cgcccccaag tgccagcagg ccgttaggct
    12121 cacgtaatgc gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc
    12181 gcatgaccgc aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt
    12241 gaactggtaa taacgcccct gtctggcaag cagctctgcg tgagtacctt gctcaataat
    12301 ttgcccgttg tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt
    12361 gaccattaac accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc
    12421 ggttgtggca tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa
    12481 cagcgcacgg gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc
    12541 acctaaccaa ctgttgagac ctgcatcctc gagcagcttc tccagaccaa cgcgacgcaa
    12601 gctctccgcc agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag
    12661 cgtggcgcta aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc
    12721 ttcattcagg ctggctatgg ggctatcgtt aagcaaaatc tctccctgtt gcgggtccca
    12781 agcgcgggtc agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc
    12841 aatatgttcc ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc
    12901 cggataagtg aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg
    12961 ggtatcaggg aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga
    13021 ggcaatgact tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc
    13081 ggctaacgcg cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac
    13141 gccgccagac gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat
    13201 cgcttgcgac aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc
    13261 tgtattctct agttgcgtgc gataacgatc gctggcgcca aaaatggtca gctcagcttg
    13321 cccttgcagc caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg
    13381 cccggtgctt tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat
    13441 aatgccgccc agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac
    13501 cataatcacc acaaaagcgc ccaccagcgg cgagataacg cgcagataaa gatgatcgag
    13561 cgtatcaaca tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc
    13621 ggcaggggag aggggcagca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg
    13681 gaaagtcgcg tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc
    13741 tgcgccacgc acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc
    13801 cgcaaccgct gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag
    13861 cagcgtgaca attgccagca cgataccaag acttaacatc catttatgac gtttatacag
    13921 tgccagatag ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt
    13981 ggcgaatggg ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatccggcc
    14041 atcctgcata acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat
    14101 taacgttgtc tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg
    14161 agcatcaagg ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc
    14221 acgggccacc gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac
    14281 aggtgtatca acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag
    14341 cgctgcttgt aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgcaa
    14401 tgttgctgcc ggtaattgtg ggttttgccc aacccaggag agatgtttac gccatgattc
    14461 tggtgataaa tcgcgtaatt ctatcccgtt gattcgtaat gatccctgat atgagagaaa
    14521 accagaaagc gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc
    14581 acgttggcct gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga
    14641 cgtgataaac agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc
    14701 gccacgctgc ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc
    14761 aacagcctgg gctttagcat gataaaacgt accgagatcg cgcaatggct ggaaaaactc
    14821 tggcgcaagg atcagggcca gaaaacccgc agccagcgtc acaccggtat cgtagtgacc
    14881 aaaatccagc tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag
    14941 cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc agccgtagca cttccattgt
    15001 ccgttggcgg aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc
    15061 aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc
    15121 aagagcgaga aagttacgcc ggttagcatc ggcagccccc atcccaacca gcgccataaa
    15181 caacggaatt aacggtgctg tgcccagcag aatgagcgcc gcagcccagt tagaggggaa
    15241 gatcgccacc acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg
    15301 tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc aacgtcgccc agctccccgc
    15361 aggtttaccc tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg
    15421 gatggcaaag cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac
    15481 ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg
    15541 gggaatattc tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat
    15601 gatcaatatg ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat
    15661 gacgctttgc tgttttaacc agcgggttaa ctctttttga cgggatttat tcattgcacg
    15721 cttagcaggt gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa
    15781 ggcgacccat agtcgcatgg tgtcgccttc tttacttttg ttactgattt gtaaaattat
    15841 tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta
    15901 ccggccgaag taatggcctg acgataaata tgatccatca cgtcacccgc ggcaaagacg
    15961 ccaggaatgc tggtctgggt ggcattacca tgaatacccg actgcacttt gatgtagccg
    16021 ttttccagtt ccagctgccc ttcgaaaatg gcagtattcg ggctgtgacc gatagcaaca
    16081 aacagaccag caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga
    16141 acgccagtga cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga
    16201 atgatgttgc cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg
    16261 aaaccgtcac ggcggtgaat aagatgcact tccgaagcga tgttagacag atacagcgcc
    16321 tcttcaaccg cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa
    16381 ccgtcgcagg ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg
    16441 ccgagatagc gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg
    16501 ttatcgccat tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa
    16561 atgatctcag tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg
    16621 gtcagatcgt ttggatcgcc aggccagttt tccacttccg tggtggtggt cagttggccg
    16681 cctttttcca tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca
    16741 gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc
    16801 atgagatccc catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta
    16861 aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt
    16921 caaattctcc cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa
    16981 atgatgaata aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt
    17041 tgctttgaca atcccctggt gttttgcgaa aacattcgag gaagaaaaaa acagtattct
    17101 tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa atctacgtat
    17161 ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg tcttctcacc
    17221 gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc
    17281 gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac agagagacaa
    17341 taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca
    17401 ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct aaacgtgtgg
    17461 gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc
    17521 agggctatac ggcgctgctg aacccccatt atctggatgc atcacttctg gtattcgttg
    17581 agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc gctgtacaaa
    17641 aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac ctgttgaaaa
    17701 cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg ctgcgtctgc
    17761 ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag agtaatcgtc
    17821 tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt gattacactc
    17881 ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg ttttagcatc
    17941 gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca ctgaagacaa
    18001 agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt tgctgatcct
    18061 tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc cttcggaccc
    18121 cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga tgcccggtgc
    18181 gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca ttcccgtcat
    18241 tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat acattgatta
    18301 ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct cctgtggtct
    18361 ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca ttggcagctt
    18421 actaagtact acgctacaac cactgctaca cagtagcggg ggaactattg cgctgctctg
    18481 cgtttgggct gcgggcctga cgctgttcac cggttggtca tgggtgacca ttgctgaaaa
    18541 actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc gtcgcgatga
    18601 tacctgggtc gatgaagatg aatatgaaga tgacgaagag tatgaagatg aaaaccacgg
    18661 caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg
    18721 gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt tgttctccgg
    18781 taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg ctgccgaccc
    18841 ggacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc
    18901 attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg ctaccacggc
    18961 gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg ttgcctctgt
    19021 tgatgttcca ccctcgcaac ctacagtagc ctggcagcct gtaccgggtc cacaaacggg
    19081 agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat atgcgcagcc
    19141 tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc cgtattatgc
    19201 acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg aacaacctgt
    19261 tcagcaaccg tattacgcaa ctgcgccgga acagcctgca caacagccgt attacgcccc
    19321 tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt
    19381 tgctccacag tctacatacc agactgagca aacttatcag caaccagccg ctcaggagcc
    19441 gttgtaccaa cagccgcaat ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt
    19501 agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg aagagaagcg
    19561 agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga
    19621 accagaaccg atcaaatctt cgttgaaagc gccttctgtt gcagcagtac ctccagtaga
    19681 agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg
    19741 ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc
    19801 gcaagtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac
    19861 tcgtcgtgaa ctggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagagaa
    19921 agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg atgatgaaat
    19981 cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta
    20041 tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga
    20101 ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc
    20161 ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct
    20221 ggatgatggt ccacacgaac cgttgtttac gccaattgtt gaacctgtac agcagccgca
    20281 acaaccggtt gcaccgcagc agcagtatca gcagccgcaa caaccagttc cgccacagca
    20341 gcaatatcag cagccgcaac agccggttgc gccgcagccg cagtatcagc agccacaaca
    20401 gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc cacagccgca
    20461 atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc cgcaacaacc
    20521 ggttgcaccg cagcagcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag
    20581 ccgtccgttg cataaaccga cgacgccgct gccttctctg gatttgctga caccgccgcc
    20641 gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggctcgcc tggtggaagc
    20701 gcgtctggct gatttccgta ttaaagccga tgtcgtcaat tactctccgg ggccggttat
    20761 cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc
    20821 acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg
    20881 caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga
    20941 agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa
    21001 agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc
    21061 ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta
    21121 taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc
    21181 ggtttatgaa ggcattccgc atctgttaac ggaagtcgtt actgatatga aagatgccgc
    21241 caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgt tataaactga tgtctgcgct
    21301 gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg
    21361 tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct
    21421 gaaaaaagaa ccatacattg tggtgttggt tgacgaattt gccgacctga tgatgacggt
    21481 aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg ccgcgggtat
    21541 ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc
    21601 gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct
    21661 tgatcaggct ggcgcggaat cactgctggg gatgggggat atgctctact ctgggccgaa
    21721 ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt
    21781 ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtg gatggcatca cttccgacag
    21841 tgaaagcgaa ggtggcgcgg gcggtttcga tggcgctgaa gaactggatc cgttgttcga
    21901 tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacagcgtca
    21961 gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat
    22021 tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta
    22081 attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa
    22141 ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac
    22201 tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgcc atcacctgtg
    22261 cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa
    22321 gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta
    22381 gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca
    22441 actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt
    22501 tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata
    22561 cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac
    22621 agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca
    22681 ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc
    22741 agcgcagcag ttatcaactg aaatcccagc aaaatggggc tgtggatgca gcgaaattta
    22801 ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg
    22861 agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg
    22921 ccagaaaatt tagcacagta tatcggccag caacatttgc tggctgcggg gaagccgttg
    22981 ccgcgcgcta tcgaagccgg gcatttacat tctatgatcc tctgggggcc accgggtacc
    23041 ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt
    23101 tctgccgtca cctctggcgt gaaagaaatt cgcgaggcga tcgagcgcgc ccggcaaaat
    23161 cgcaatgcag gtcgccgcac tattcttttt gttgatgaag ttcaccgttt caacaaaagc
    23221 cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc
    23281 actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtctatcta
    23341 ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa
    23401 acccgtggct atggtggtca ggatattgtt ctgccagatg aaacacgacg cgccattgct
    23461 gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg
    23521 gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg aattactgac cgaaatcgca
    23581 ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct gatttccgca
    23641 ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tctactggta tgcgcgaatt
    23701 attaccgccg gtggcgatcc gttatatgtc gcgcgtcgtt gcctggcaat tgcttccgaa
    23761 gatgtcggca atgccgaccc acgggcgatg caggtggcaa ttgcggcctg ggattgcttt
    23821 actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctagcc
    23881 tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc ggatgctcgc
    23941 gaacgtccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag
    24001 gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc ttatgctgcc
    24061 ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg
    24121 ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga tcaaaatagc
    24181 cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg
    24241 tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat
    24301 cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt
    24361 aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa
    24421 acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg
    24481 cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat
    24541 gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc
    24601 cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgtcgaagtc
    24661 agccgctggg gcaccccgcg tgagtttgac tttgaagttc gtgaccatgt gacgctgggt
    24721 gaaatgcact ctggcctcga ctttgcagcc gcagttaagc tgaccggttc ccgctttgtg
    24781 gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg
    24841 cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg
    24901 ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg
    24961 gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact
    25021 aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat gaccgcccac
    25081 accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt
    25141 atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga ctcaatggcg
    25201 gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac
    25261 cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg
    25321 gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc caacgtttgg
    25381 gatttccagg cacgtcgtat gcaggcacgt tgtcgcagca agtcggacaa gaaaacccgt
    25441 ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcggttatg
    25501 gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg
    25561 aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag cgcctgcggg
    25621 cgcttttttt gtctcccttt gataccgaac aataattact cctcacttac acgtaatact
    25681 actttcgagt gaaaatctac ctatctcttt gattttcaaa ttattcgatg tatacaagcc
    25741 tatatagcga actgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt
    25801 gagcaagctt gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga
    25861 ggtgagtcgc cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag
    25921 cgcattaaca ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa
    25981 atcagacgaa aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct
    26041 acgtatgcac gtcgtggacg gtgaaatcaa atatgtcgaa acggacaata ccggcgatga
    26101 caattacgac ggcctgcacc aggttcgtgc ctgtctgcgt gggcgttcca tgcgtcgccg
    26161 tgtctacaat ccggaccgcc tgaaatatcc gatgaaacga gtcggggcgc gcggtgaagg
    26221 caaattcgag cgcattagct gggaagaagc ctacgacatc atcgcgacca atatgcagcg
    26281 cctgatcaaa gagtacggca acgagtctat ctatctgaac tatggcaccg gtacgctggg
    26341 cggcaccatg acccgctcct ggccgccggg aaataccctg gtcgcgcgcc tgatgaactg
    26401 ctgcggcggt tatcttaacc attacggtga ctactcttct gcgcaaattg cggaaggttt
    26461 gaactatacc tacggcggct gggcagatgg taacagcccg tcggatatcg aaaacagtaa
    26521 attggtagtg ctgtttggta ataacccagg cgaaacgcga atgagtggcg gtggggtgac
    26581 ttactatctt gaacaggcac gccagaaatc taatgcccgc atgatcatca tcgatccgcg
    26641 ctataccgac accggtgccg ggcgcgaaga tgagtggatc cctattcgtc caggaacaga
    26701 tgccgcactg gttaacggtc tggcgtatgt catgatcacc gaaaacctgg tggatcaggc
    26761 attccttgat aaatattgcg ttggctacga tgagaaaacc ctgcccgcta gcgcgccgaa
    26821 aaatggccac tataaagctt atattctggg tgaagggcca gatggcgtgg ctaaaacacc
    26881 gcaatgggcc tcgcaaatca ccggtatccc ggcagagaaa atcattcaac tggcacggga
    26941 aatcggcagt accaaaccgg cgtttatcag ccagggatgg gggccacagc gccacgccaa
    27001 cggcgaaatc gcgactcgcg ctatctcgat gctggcgatt ctgaccggta acgtcggcat
    27061 taacggcggt aacaccggcg cgcgtgaagg ttcctatagc ctgccgtttg tccgtatgcc
    27121 gaccctcgaa aacccgatcc agaccagcat ttctatgttt atgtggaccg atgccattga
    27181 acgtggcccg gaaatgacgg cgctacgtga tggtgtgcgc gggaaagata agctggatgt
    27241 gccgatcaaa atgatctgga actatgccgg taattgcctg attaaccagc attctgaaat
    27301 caaccgtacc catgaaatcc ttcaggatga taagaagtgc gagctgattg tggttatcga
    27361 ctgccacatg acctcatcgg cgaaatatgc tgacatcctg ctgcctgact gcaccgcttc
    27421 cgaacagatg gactttgcac tggatgcatc ctgcgggaat atgtcttacg tgattttcaa
    27481 cgatcaggtg attaaaccgc gctttgaatg taaaaccatc tatgaaatga ccagcgaact
    27541 ggcaaaacgt cttggcgttg agcaacagtt tactgaaggc cgtacacagg aagagtggat
    27601 gcggcatctg tatgcccagt cgcgggaagc gattcctgaa ctgccaacgt ttgaagagtt
    27661 ccgcaagcag gggatcttta aaaagcgcga cccacaaggg catcacgttg cttataaagc
    27721 cttccgtgaa gatccgcagg caaacccact gactacgcca tcgggcaaaa ttgagattta
    27781 ttcgcaggcg ctggctgaca ttgccgctac ctgggaattg ccagaaggcg atgtgatcga
    27841 tccactgccg atctacacgc cgggctttga aagttatcag gatccgctga acaaacagta
    27901 tccgctgcag cttacgggtt tccactataa atctcgcgtt cactcaactt acggcaacgt
    27961 tgatgtgctg aaagcggctt gccgtcagga aatgtggatc aacccgcttg atgcgcaaaa
    28021 acgcggtatc aacaacggcg ataaagtcag gatctttaac gatcgtggtg aagttcatat
    28081 tgaggcgaaa gtgacgccac gaatgatgcc gggtgtggtc gcactgggtg aaggtgcctg
    28141 gtatgacccg gatgcaaaac gtgtcgataa gggcggttgt attaacgtac tgaccactca
    28201 acgtccgtct cctctcgcta aggggaatcc gtcacataca aaccttgttc aggttgaaaa
    28261 ggtgtaagga gtaaccgatg acaacccagt atggattttt tattgattcc agccgttgca
    28321 ccggttgcaa aacctgcgag ctggcctgta aagactacaa agatttgacg ccagaagtca
    28381 gcttccgccg catttatgaa tatgctggcg gcgactggca ggaagataac ggtgtctggc
    28441 accagaacgt gtttgcctac tatctgtcga tttcatgtaa ccactgcgaa gatccggctt
    28501 gtactaaagt ctgcccgagc ggtgcgatgc ataaacgcga agatggtttt gttgtggtcg
    28561 atgaagatgt gtgcattggc tgccgctact gccatatggc ttgcccgtat ggcgcaccgc
    28621 aatataacga aacgaaaggc catatgacca aatgcgatgg ttgttatgac cgtgttgcgg
    28681 agggtaaaaa gccgatctgt gttgaatctt gtccgctgcg ggcgctggat ttcggcccta
    28741 tcgacgagct gcgtaaaaaa catggcgatc tggcggccgt tgcgccgttg ccgcgagctc
    28801 actttaccaa accgaatatt gtgatcaaac ccaatgccaa tagccgcccg accggggata
    28861 ccactggcta tctggcaaac ccgaaggagg tgtgagatgg gaagtggatg gcatgaatgg
    28921 ccgctgatga tcttcacggt cttcgggcaa tgtgtagcag gtggttttat cgttctggct
    28981 ttggcgctgc tcaaaggcga cctgcgagca gaagcccagc agcgtgttat cgcctgcatg
    29041 tttggtttat gggtgctgat gggcattggc tttatcgcct ctatgctcca tcttggttca
    29101 ccaatgcgcg cttttaactc gctcaaccgg gtaggggctt cagcactcag taacgaaatc
    29161 gccagcggtt cgatcttttt tgccgtaggc ggcatcggct ggttgctggc aatgctgaaa
    29221 aagctgtcac cggcattgcg tacgctgtgg ctgatagtga cgatggttct tggcgtcatc
    29281 tttgtctgga tgatggtgcg tgtgtataac agcattgata ccgttccgac ctggtacagc
    29341 atctggacgc cgatgggctt cttcctgacg atgtttatgg gcggcccgtt actgggttac
    29401 ctgttgttga gtctggctgg cgtcgatggc tgggcgatgc gtctgctgcc agcaatttct
    29461 gtactggcac tggtagtgag tggcgtggtg tcagtgatgc agggcgcaga gctggcgact
    29521 attcatagtt ctgtgcagca ggccgcagcg ctggtgccgg actatggtgc gctgatgtcc
    29581 tggcggatcg tgcttttggc cgttgccctg tgcttgtgga ttgcaccaca gctaaaaggt
    29641 tatcagcctg cggttccgct actatccgtc tcattcattc tgcttctggc aggggaatta
    29701 atcggtcgcg gcgtattcta tggtttgcat atgaccgtgg ggatggccgt cgcaagctaa
    29761 tcagtaacaa ccggggtttc ggccccggtt ctcttttatt tctgcttcgt taacgtgtca
    29821 taactggtca tcaaattacg ataatcagga atgtggttag agaacaacgt cgccagtcct
    29881 tcaatatcat tacgccagtc gcgatgcagc tcgcacgcca cgccgaacca tgtcatcaat
    29941 tgcgcaccag cttgcgacat gcgatcccat gccgaatgcc gggtaatttc attaaaggtc
    30001 cctgacgcgt cagtcacgac aaagacgtca aacccttctt caatggctga aagcgccggg
    30061 aatgccacgc aaacttcggt taccacaccg gcaataatta actgtttttt acctgtcgct
    30121 ttgacagctt ttacaaaatc ttcgttatcc caggcgttaa tatttcccgg gcgagcaatg
    30181 taaggtgtat cggggaattg tgctttcagt tctggaacta atgggccgtt aggaccggtt
    30241 tcaaaactgg tggtaagaat agttggtaag ttgaaatact tagccaggtc acccagcgcc
    30301 agcacgttat ttttaaactt atcaggttcg atatcccgta caagggaaag taaaccagcc
    30361 tggtgatcaa caagcaaaac ggcagcatca tttttatcaa gacgaacata cggtttggtc
    30421 atcctcttct cctttcgaga gtcagtggta cgtctgaaat cgatcagacg cgatgcattg
    30481 ctctgaaagc atagacggga aatatgagtt tgctgtgacc atgaaatttt tcgactgaac
    30541 gaagcgtatg aaatttgtgt tagttcaata aaaacaatca gatgagacta atcatctctt
    30601 cagcattagc gttatgaatg agtaatataa atcgataata ccgccgctgg gatatcgcgt
    30661 attttcaccc attgacaatg tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc
    30721 tcacggtttt aattcatgtc cacgtatacc cggcctgtca tgcttttgct gtctggcctg
    30781 cttttgttga ctctggcgat tgcggtgtta aatacactcg tgccgctttg gctcgcccag
    30841 gaacacatgt ccacatggca ggtaggcgtt gtcagctcat cctattttac cggcaacctt
    30901 gtcggtacat tgctgacagg gtatgtcatt aagcgcattg gctttaaccg cagctattat
    30961 ctggcctcct tcatttttgc cgctggctgt gccggccttg gcctgatgat tggattctgg
    31021 agctggttgg cttggcgttt tgtcgcgggc gtcggctgtg ccatgatttg ggtggttgtt
    31081 gagagcgcgc tgatgtgcag tgggacgtca cgtaaccgtg ggcgtttgct tgctgcgtat
    31141 atgatggttt attacgtggg aacgttttta ggccagttac tggtcagcaa agtttcaacc
    31201 gagctgatgt ccgtattgcc gtgggttaca ggtttgacgt tggcagggat cttaccgctg
    31261 ttgtttacgc gtgtgctgaa tcagcaggct gaaaaccatg attcgacgtc aattacgtca
    31321 atgctaaaac tccgtcaggc gcggcttggc gtgaatggct gcattatctc aggaatcgtt
    31381 ctgggatctc tatatggcct gatgccgctg tacctcaatc acaaaggggt gagcaatgcc
    31441 agcattggtt tctggatggc ggtactggtc agtgcgggta tccttggaca atggccgatt
    31501 ggacgtctgg cggataagtt tggtcgactg ctggtgttgc gtgttcaggt ctttgtcgtc
    31561 attctcggca gtatcgcgat gcttagccag gcggcgatgg ccccagcgtt attcatcctc
    31621 ggtgccgctg gctttacgct atatccggtg gcgatggcat gggcttgcga gaaagttgaa
    31681 catcatcaac tggtggcgat gaaccaggcc ttactgttga gctatactgt gggaagtctg
    31741 cttggcccgt catttaccgc tatgctaatg cagaatttct ccgataattt attgtttatc
    31801 atgatcgcca gcgtatcgtt tatctatttg ctgatgctgc tgcgcaacgc cggtcatacg
    31861 ccgaaacccg ttgctcacgt gtaaatgaat tcaagcagag tgtgaactta ctgtttcaca
    31921 ctctgctttt ttgtttcttc tatctgactt gctttattcc aaattttatt cgtttaaaaa
    31981 taaaatgtgc agcaggttat aattttgcat ttcgctattt ccgcacttct tatttgccgc
    32041 gcataatccc tcgttttaca gatgcccctt taattttggc gaaggatttg tctatggctg
    32101 ggaatgttca ggaaaaacag ctgcgatggt acaacattgc gctgatgtct tttatcactg
    32161 tctggggttt tggcaacgtt gttaataact acgccaacca ggggctggtg gttgtttttt
    32221 catgggtgtt tatctttgcg ctctatttca caccttatgc gctaattgtt ggtcagttag
    32281 gctcgacctt caaagatggg aggggcgggg tcagtacctg gattaaacac acgatgggac
    32341 ccggactggc ttacctcgcc gcgtggacct actgggtagt gcatattccc tatctggcac
    32401 aaaaacccca ggcaatactg attgcgctcg gttgggcgat gaaaggcgac ggttcgttaa
    32461 tcaaagaata ttcagtcgta gcgttacagg ggttaacgct ggtgctgttt atcttcttta
    32521 tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt cggttctgtg gcagggattg
    32581 cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt aaccgcgcct gcaattactg
    32641 aagtgcatat tgcgaccaca aacattacct gggaaacgtt cattcctcat atcgacttta
    32701 cctacattac cactatttca atgctggttt tcgcggttgg cggagcagag aagatttctc
    32761 cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc aaaagggatg ttatgcctgg
    32821 cggtgatggt tgcggtttgt gccattctgg gctcgctggc gatggggatg atgtttgatt
    32881 cgcgtaatat cccggatgac ttaatgacca acggtcagta ttacgccttt cagaagctgg
    32941 gcgagtatta caacatgggt aatactttaa tggtgattta cgccattgcg aataccctgg
    33001 gacaagtagc ggcgctggta ttctcgattg atgccccgct taaagtgcta ttaggtgatg
    33061 ctgacagcaa atatattcca gccagtttat gtcgtaccaa cgcttctggt acgcccgtta
    33121 atggctattt tctgaccctg gtactggtgg cgattctgat tatgctgccg actctcggca
    33181 ttggtgatat gaacaatctc tataaatggc tgttgaacct taattcggta gtgatgccgc
    33241 tgcgttattt atgggtattt gttgcattta ttgcagtcgt tcgcctggcg cagaaatata
    33301 aaccagaata tgtctttatt cgtaataagc ctctggcaat gaccgtcggg atttggtgtt
    33361 ttgcctttac cgcctttgcc tgtttgacgg ggatcttccc gaaaatggaa gccttcactg
    33421 cagagtggac cttccagttg gcgctgaatg ttgcaacgcc gtttgtgctg gtaggattag
    33481 gactgatttt cccgctgctg gcgcgtaaag cgaatagtaa ataattattg tggtcggtga
    33541 cgctgactct ggcagatctg acccgttttt acataatcga gaaatgcgcg caagccgcag
    33601 gacatatact ggcggtttgg ataatagatc tggaagcctg gacgctctgt gctccagtct
    33661 tccagtacac attctaaacg accagtatca aggtactcct tgatctgttc atagagcaga
    33721 tacccgatac cagcacccat caaaacggcc tccagttcag catcaacatc atccagaatg
    33781 atatttcctg cgacagcaat ttccagctcc tttgcaaatt gccagtggaa aggtttgccg
    33841 ctgggataac gaaaaacaac acattgatga ttaagcagat catgaggatg gcggggtttg
    33901 ccatatcttg caaagtactc tggcgttgct gctacacata atttcactgg cgggccgatc
    33961 gccacgctga tcatatcttt ttcgacgatg ccactaagtc gaacgccagc gtcaaagccc
    34021 tgttggacga tatcgacaag agagtcgtca gttgtgagct cgactttaat atcaggatat
    34081 tcacgtgtga aaccaaccag taaaggcatt aaaaatatac gtgcagctac ccgggcggca
    34141 ttgattttga gcgttcctgt cggtgtcaga cgaaaatcat tcatctcatc taacatgatc
    34201 tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat tagaacccgc ttccgttaac
    34261 gatacgcttc gggttgtccg attgaataag cgaattttaa gacgttgttc cagtgtttta
    34321 atactatggc taatggcgga cgaggataag cctaactcat cgcccgctgc acgaaagctt
    34381 tgattacggg ccacggcaaa gaaagtggca aagtcagaca tattcatccg cattagtgaa
    34441 aatccttcat taactcatcc tgaatcatca cgtttacagg attatgccgc ctgcgtaaag
    34501 tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt tatttggtat
    34561 gggcccatcc tcgtcatgat tcattgaccg cacatattgc tgatgcgatc catcagcggg
    34621 caatggagcg gaaaatacag gtgacggaac tcgatttata tcggcgtaat ttcaacccag
    34681 tgatgacgcc ggaagatgaa ccagactgga agaatatgga taaacgttat tctccagagg
    34741 ttcatcagct ttattcagag ctgcttgaac atgacacgtt agtggtggtt tttcctctct
    34801 ggtggtacag cttcccggca atgctaaaag gatatattga cagagtatgg aataatgggc
    34861 tggcttatgg agatgggcac aaattaccat tcaataaagt tcgttgggtg gcgctggttg
    34921 gaggagacaa agaatcattt gtccagatgg gctgggaaaa aaatataagc gattatttaa
    34981 aaaatatgtg cagttatctt ggtattgaag atgccgatgt cactttcttg tgtaatacag
    35041 tggtattcga tggggaagaa cttcacgcga gctattatca gtcgttatta tctcaggtac
    35101 gggatatggt agatgcacta taagatgtgt taaaaacgct gtagcagaat gaagcgcgga
    35161 ataaaaaagc ggcaactcaa taaagttgcc gctttacggg gaaattagaa cattacctta
    35221 tgaccgtact gctcaagaat gcctttcacg cgttccatgg tctctttctt cggtggttta
    35281 acaccgtcga gtttgtactc ttcacccatt gccacccatt tgtgtttgcc cagctcgtgg
    35341 taggggagaa gctcgatttt ctcaacgttg cccatatcac gggtaaattc accgaggcga
    35401 tgcgctgaat cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc
    35461 acatttttgt tcgccagata tttagcgaac tccagcgtgc ggtggttgga aactccaacc
    35521 agattttggt ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact
    35581 tccagcagtt catcaatcac cggatcgtaa cgacgaacaa aaccgttggt gtccagacag
    35641 gtatgaatgc cttctttttt gcaggcgcgg aaccagtcac gaacaaactc agcttgcagg
    35701 attgcttcac cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag
    35761 gtcaccactt ccttcatcaa atcttcaacg gtaacttctt taccgccatg cgtgtcccag
    35821 gtgtcgcggt tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag
    35881 cgaatacctg ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact
    35941 gacattgcgg tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa
    36001 ggctacgtcg agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct
    36061 ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga
    36121 tttcagtcaa atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct
    36181 gttctttagt cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg
    36241 gatatttttc cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt
    36301 tcaggtgctg accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagacctg
    36361 ccaggttggt cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg
    36421 tgtaggagat accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg
    36481 ctacagcacc tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc
    36541 cagcacgacg accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga
    36601 tggtcagaac agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct
    36661 tcatgaaacg ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa
    36721 actgcgggta ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg
    36781 gtttaacttt cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga
    36841 taccacacgc catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt
    36901 agctgtactt gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca
    36961 gccagtccat gaagtgatcc atgcgctcca tcacttcatc gtagttcagg agatcgcctt
    37021 tgatcggttc agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga
    37081 ttgcgtacag catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac
    37141 caacgatcat cgggcttacg cagcaagcga tagcatagtc atcgttgttg aagtccggac
    37201 gcatcagatc atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga
    37261 atttcttgaa gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag
    37321 acggacccat ggtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac
    37381 gaccgtcgag gcccatacca ccgatagatt cggttgccca gatcgggtcg ccagagaaca
    37441 gttcatcgta ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt
    37501 caaccatttc ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt
    37561 acacatccag gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa
    37621 cagcagccag gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac
    37681 cagagatgtc gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct
    37741 gttcagcgat ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat
    37801 cagtctgcag agaggtgaac tgagcgtatt tgtctttcat caggtagtcg ataccgtaca
    37861 gcgcaacgcg acggtagtca ccgatgatac ggccacggcc ataagcatct ggcagaccgg
    37921 tcagaacacc ggatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct
    37981 ggttgtgagt tttacggtat tcagtgaaga tttttttgat catcgggtcc agttcgcggt
    38041 tgtacgcttt gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt
    38101 tcagcggagc ttcagtctgc agaccaacaa ctttttccaa cgctttgttg atgtaaccag
    38161 cgtcgtgaga ggtgatggta gaagcaacag cggtgtcaaa gtcaactggc gcgtgagtgc
    38221 ggttttccag tttaacgcct tccattactt tgtcccacag ggtggtggtc gcttcagtag
    38281 cgccagccag gaaggactcg tcaccctcgt acggagtgta gtttttctga atgaagtcac
    38341 ggacgtttac ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact
    38401 tttcattaag ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt
    38461 acttcgacaa ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc
    38521 caaccaacaa accaccgccg ataatattac cgatcgtaac cggaatcagg ttatcagtga
    38581 tgaagttcat cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga
    38641 attccggaga tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga
    38701 tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt
    38761 tgtccatcag gctacggcca gaataactca tccatactgc cagacatacc atcaggtttg
    38821 ccaggatacc aaggcagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt
    38881 gtaggacgtt tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta
    38941 aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttcg
    39001 ccaactgacc ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag
    39061 taaagagatc ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc
    39121 caaccagttt tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga
    39181 agacgaatgc gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg
    39241 gatgtttcgt tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa
    39301 gtaaaagatc aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac
    39361 gagatactaa caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta
    39421 taggcccgta actcgcatgg tttttatgca aatacggagt aaatatttga ttatccaaat
    39481 aaaaataaat tttaaaaatt aacaaatga
//
LOCUS       DAPHJE010000035        38598 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPHJE010000035 DAPHJE010000000
VERSION     DAPHJE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.035"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(22..264)
                     /locus_tag="RH222_001794"
     CDS             complement(22..264)
                     /locus_tag="RH222_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HDV3796834.1"
                     /translation="MGAYLGSKVKGEDPTVPMVGNAIGTVLGNKAGEKFTKEMLSKGY
                     SPVTSDVIGTVSGGTIGTAIDFGIEESQKKGDSNEK"
     gene            complement(732..962)
                     /locus_tag="RH222_001795"
     CDS             complement(732..962)
                     /locus_tag="RH222_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796835.1"
                     /translation="MKLFIFSLFVIVTSMVSGIAIAELSYFILLIIKYLAYGEVDFRW
                     SEVLRGLRMGCVGGGILGFGIVLFRFLGIKGF"
     gene            complement(959..1447)
                     /locus_tag="RH222_001796"
     CDS             complement(959..1447)
                     /locus_tag="RH222_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV3796836.1"
                     /translation="MWLLGAGTNAGMQYADSGEINPVNSVAAGWINVITMGQGWKGTI
                     AWNAAGGALTNAINGDDPLTGAITNGAGAGFGYGVGNYVVKPVANTAGKWITGGWNPK
                     FDPTLLKYTEVKGQLGISKEMVPSKIPGAAGNMGASITSEMTGTELQKVINDKLKKGE
                     RE"
     gene            complement(1679..2134)
                     /locus_tag="RH222_001797"
     CDS             complement(1679..2134)
                     /locus_tag="RH222_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796837.1"
                     /translation="MMYLLLGIALSAFTVGTVMFYRFSKVTGRSVQSPFTPMFFFDAL
                     FFGLGSRRDMNIAACAFMVFLLSVWGLTYIKLRTNFWGSPESYFGLGIVGIFFLIMHC
                     RYGAKDVVIKNDGLAPIRELINVHQSGLTSPFLWLSRAGYLGCLIGMIG"
     gene            complement(2139..2648)
                     /locus_tag="RH222_001798"
     CDS             complement(2139..2648)
                     /locus_tag="RH222_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796838.1"
                     /translation="MPPRVQQDASLAFDLSGKGVTNDEIIKAVDKSHIGPSVGDTAKI
                     HGDVKGQVAGGYVAGGYLEGVLSEDKFSINGGVTKVLGWRADVSAGLTFGPYPIKDFN
                     PTYDYSGAISTGIFSVEGSIGKDGFGGSFRVGGGIGASIRQSENSNNQPEINGSGSTE
                     LISWPIKKD"
     gene            complement(2671..2928)
                     /locus_tag="RH222_001799"
     CDS             complement(2671..2928)
                     /locus_tag="RH222_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796839.1"
                     /translation="MKYSSRVVIGKFIICSSILLLCFIGMILAAIITKLTFVALAWFQ
                     SGIFEVLWSDIVYAAQLGGLGGGILGFCWVLLYLFRAKGFS"
     gene            complement(2925..12698)
                     /gene="cdiA"
                     /locus_tag="RH222_001800"
     CDS             complement(2925..12698)
                     /gene="cdiA"
                     /locus_tag="RH222_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition effector tRNA
                     nuclease"
                     /protein_id="HDV3796840.1"
                     /translation="MNQPPVHFTYRLLSYLVSAIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRITADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSDIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTVASDTLTTQG
                     QITAGRNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNSGSL
                     LSGGPLTVNTRDFTQSGRTGAKGKVDITASGKLTSTGSLVSDDVLVLKAQDVTQNGVL
                     SGGKGLTVSAQALSSGKKSVTHSDAAMTLNVTTVALDGENSAGDTLLVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMAVNASEKLTHSGKSSAPSLSLSAPELTSSG
                     VLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITSDNGLTLNTASLSNPGKIIADTLNVRATTLDGDGLLQGAGALALAGDTLSQGRNG
                     RWLTAGDLSLRGKTLHTAGTTQGQNLTVQADNWANSGSVLATGNLTASATGQLTSTGD
                     IMSQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNRGTVQGDHVTIRQNSVTNSGTFT
                     GIAALTLAARMVSPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITADSLAN
                     SGAIQAADSLTARLTGELVSAAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLT
                     SAGDITGVDALTLTVNQTLNNHASGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQL
                     TNGGHLQGETLTLAASGGVNNRSGGVLMSRNALNVSTATLSNQGTIQGGGGVSLNATD
                     RLQNDGKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVKGT
                     TLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLDAQ
                     DFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAG
                     EAFTNNGMLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLT
                     MNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWIQKDASGGANTEIINTSG
                     NIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGY
                     FTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGR
                     IASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFY
                     GSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSI
                     NNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGA
                     LKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHL
                     FAGLYELPGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTR
                     YVSNAVLSRTGSRYINGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGS
                     ILWWESATINGQTVMVPKLYLSPEEITLHNGSVISGNNVQLAGGNITNSGGSINAQNG
                     LSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRR
                     QQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGMSA
                     GNDINIAANLISGSKSQSGFWHTDDNSASSTTSQGSSISAGGNLAMAAGHNLDVTASS
                     VSAGHSALLSAGNDLSLNAVRESKNSRNGRSESHESHAAVSTVTAGDNLLLVAGRDVA
                     SQAAGVAAENNVVIRGGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDT
                     TVNAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTH
                     TISEDSASREAGSLLSGNRVTVNAGDNLTVEGSDVVADQDVSLAAGNHVDVLAATSTD
                     TSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAG
                     KQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAIN
                     NAVTSAQETKESSDSRLKVLQATKTALSGVQAGQAAAMATATGDPNATGVSLSLTTQK
                     SKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAAN
                     DVLLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVNAAKGSEKGNGTEWT
                     ETTIDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAA
                     GGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIA
                     STATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGG
                     HSGHAEGTTQAAVADGTITIRDRDNQKQNLANLSRDPAHANDSISPIFDKEKEQRRLQ
                     TVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQRQEYLAKLRDTQAY
                     RNEMAKYGTGSEIQRGIQAATAALQGLVGGNLAGALAGASAPELAHLLKSTEKYPAVN
                     AIAHAILGGAAAAMQGHNVAAGAAGAATGELAARAIAGMLYPGVKQSDLSEEQKQTIS
                     TLATVSAGLAGGLTGNSTASVAVGAQSGKNAVENNLLGGSEDAQAAWIRQHGIDMATC
                     AENPSGAACQKAMNERDAVGLALATGTVALLPGSAQAMWGLGASANAGISYLADGTVD
                     PANAAIAGWVNVFSMGNGLAGTVGWNAAGGALGNWIDNKDPLSGAISNGIGSAAGYGI
                     GKGVTWGTDKITNAVGKWVTNGWDPKYDPTWIKYAEVNRNDIIREGMKPSNVPGTAGD
                     IGGSIGSELGGKVTDKVSEELKK"
     gene            complement(12711..14483)
                     /locus_tag="RH222_001801"
     CDS             complement(12711..14483)
                     /locus_tag="RH222_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDV3796841.1"
                     /translation="MQRRQNNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITS
                     RAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTEPVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKNTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVAALDGGWLHSDRDDPYSSGTLWGGAAGLSTTSDYVSGSFTAG
                     LPLVYPDWLAPDHLTVYWRVAVAF"
     gene            14999..15388
                     /locus_tag="RH222_001802"
     CDS             14999..15388
                     /locus_tag="RH222_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796842.1"
                     /translation="MKRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            15453..16511
                     /locus_tag="RH222_001803"
     CDS             15453..16511
                     /locus_tag="RH222_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3796843.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            16552..17274
                     /locus_tag="RH222_001804"
     CDS             16552..17274
                     /locus_tag="RH222_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HDV3796844.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            17271..18092
                     /locus_tag="RH222_001805"
     CDS             17271..18092
                     /locus_tag="RH222_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDV3796845.1"
                     /translation="MSKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            18067..18324
                     /locus_tag="RH222_001806"
     CDS             18067..18324
                     /locus_tag="RH222_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3796846.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            18336..18587
                     /locus_tag="RH222_001807"
     CDS             18336..18587
                     /locus_tag="RH222_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3796847.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            18592..19173
                     /locus_tag="RH222_001808"
     CDS             18592..19173
                     /locus_tag="RH222_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796848.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            19170..20528
                     /locus_tag="RH222_001809"
     CDS             19170..20528
                     /locus_tag="RH222_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDV3796849.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            20515..20868
                     /locus_tag="RH222_001810"
     CDS             20515..20868
                     /locus_tag="RH222_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HDV3796850.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            20859..22535
                     /locus_tag="RH222_001811"
     CDS             20859..22535
                     /locus_tag="RH222_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3796851.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMCHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKINSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            22539..22961
                     /locus_tag="RH222_001812"
     CDS             22539..22961
                     /locus_tag="RH222_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDV3796852.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            22958..23563
                     /locus_tag="RH222_001813"
     CDS             22958..23563
                     /locus_tag="RH222_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HDV3796853.1"
                     /translation="MKFLPLLVLLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPQQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            23532..25850
                     /locus_tag="RH222_001814"
     CDS             23532..25850
                     /locus_tag="RH222_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HDV3796854.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDSWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            25847..26431
                     /locus_tag="RH222_001815"
     CDS             25847..26431
                     /locus_tag="RH222_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HDV3796855.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            26433..27602
                     /locus_tag="RH222_001816"
     CDS             26433..27602
                     /locus_tag="RH222_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HDV3796856.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            27599..28063
                     /locus_tag="RH222_001817"
     CDS             27599..28063
                     /locus_tag="RH222_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HDV3796857.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            28063..28794
                     /gene="fabG"
                     /locus_tag="RH222_001818"
     CDS             28063..28794
                     /gene="fabG"
                     /locus_tag="RH222_001818"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV3796858.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            28791..30020
                     /locus_tag="RH222_001819"
     CDS             28791..30020
                     /locus_tag="RH222_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDV3796859.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(30860..31891)
                     /locus_tag="RH222_001820"
     CDS             complement(30860..31891)
                     /locus_tag="RH222_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3796860.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            32162..32605
                     /locus_tag="RH222_001821"
     CDS             32162..32605
                     /locus_tag="RH222_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3796861.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            32621..32908
                     /locus_tag="RH222_001822"
     CDS             32621..32908
                     /locus_tag="RH222_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3796862.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            32921..34177
                     /locus_tag="RH222_001823"
     CDS             32921..34177
                     /locus_tag="RH222_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV3796863.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(34389..34673)
                     /locus_tag="RH222_001824"
     CDS             complement(34389..34673)
                     /locus_tag="RH222_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3796864.1"
                     /translation="MEQKTLSVEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(35095..36108)
                     /locus_tag="RH222_001825"
     CDS             complement(35095..36108)
                     /locus_tag="RH222_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDV3796865.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(36120..37436)
                     /gene="fucP"
                     /locus_tag="RH222_001826"
     CDS             complement(36120..37436)
                     /gene="fucP"
                     /locus_tag="RH222_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV3796866.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(37464..38384)
                     /gene="rbsK"
                     /locus_tag="RH222_001827"
     CDS             complement(37464..38384)
                     /gene="rbsK"
                     /locus_tag="RH222_001827"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV3796867.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
BASE COUNT         9050 a        10402 c         9565 g         9581 t
ORIGIN      
        1 aacagccata agtcaggaga tttatttttc attactatct cctttcttct gagattcttc
       61 aatgccgaag tcgatagctg tacctatagt ccccccagag acagttccaa taacatcaga
      121 agttactggc gagtatccct tcgatagcat ttctttggtg aatttctctc cggctttgtt
      181 acccagaaca gtacctattg cattccccac cattggcact gtagggtctt cgcctttaac
      241 tttacttcct aagtaagctc ccattgtgtt aataaatgtg ctgaaaatga cacttttacc
      301 ctgtgtaagc ccaccttcac ctgcggcaat aagagtatcc gtagcactat atttctcacc
      361 cggcttaagg gttaaatact gcttaatgcc atctgttaca cctcctaaaa ggctaccccc
      421 aataacagca acggtacccg cggctgggac tataagttcg ccaccaagaa cagaagcacc
      481 tgctccccat gcaatcagta aacctctgac aggatcctgt ccttccggaa gattacctgt
      541 tgcaagttta tcagaagcat acttaatttc ctctggcgtt gccggattca gcactttgcc
      601 agtttcatca gtgagattgg tattatttac cagcgtcccc tgggcctgaa cataactccg
      661 gaagccggtc ccaaaccctt ccaggccaga cattgaatta ttctcaacaa caaatcgtac
      721 cttatcttca cttaaaatcc ttttatgcca agaaagcgaa acaagactat accaaaacct
      781 agaattccac ctcccacaca ccccatcctt agacctctta aaacttcgga ccatctaaaa
      841 tcaacttccc catacgctaa gtatttaatg ataagcaata tgaaataaga caactcagca
      901 attgcaattc ctgaaaccat ggaggtaact atgacaaaga gactgaaaat aaatagtttc
      961 attcacgctc tcctttcttc aatttatcat taatgacttt ttgtagttca gtccccgtca
     1021 tttctgatgt aatagaagcc cccatattac ctgcagcccc cggaatcttg ctcggcacca
     1081 tttctttgga gatacctaac tgccctttaa cttcagtata ttttagtaga gtcggatcga
     1141 atttaggatt ccagccaccg gttatccact tgcctgctgt atttgcaaca ggcttcacca
     1201 cataattgcc aaccccatat ccaaacccgg cccccgctcc atttgtaatc gccccagtaa
     1261 gaggatcatc tccgtttata gcgttggtta atgctccacc tgccgcattc caggctattg
     1321 tccctttcca gccctgcccc atggttatca catttatcca gcctgctgcc actgagttga
     1381 ccgggtttat ttcaccacta tctgcatact gcatcccggc atttgtcccg gcaccaagta
     1441 accacatagc ctgggcactc cccggtaaca gcgaaacact acttgtcgct aatgcggctg
     1501 cataagcttt attttcagcc tgcccccgct tacaggcttc accggaagga ttatcactac
     1561 aggagagaac atcagcgcca tgctctctgg ctttttccgt ctggagccat tcactgccac
     1621 ccagcaggtt attctcaaca actaattggc atttatcaca aatttcatta acactgcatc
     1681 agcctatcat gccaattaaa catccgaggt accctgccct acttaaccac aaaaatgggc
     1741 tggtgaggcc agactgatga acattaatta actcccttat tggtgcaaga ccatcatttt
     1801 tgataacaac atcctttgca ccatagcgac aatgcattat cagaaagaaa attccgacaa
     1861 tccctaaacc aaaataactt tctgggcttc cccaaaaatt agtccgtaat tttatatagg
     1921 tgagccccca aacactcagt aaaaagacca taaaagcaca ggcagcaata ttcatatcgc
     1981 gtctgctgcc tagcccaaaa aatagagcat caaaaaaaaa cattggggta aaaggtgatt
     2041 gcacgcttct tcctgtcact ttactaaatc gataaaacat gaccgttccg acagtaaaag
     2101 cagaaagtgc aatacctaac agcaagtaca tcatttggtt agtccttttt tataggccaa
     2161 gaaattagtt cggtactacc agaaccgtta atttccggct gattattact gttttcactt
     2221 tgtcgaatac tcgcaccaat tcctccacca actctgaaag agccgccaaa gccgtcttta
     2281 ccaatactcc cttcaactga aaaaatacct gtcgatatag caccagaata atcataagta
     2341 ggattaaaat ctttaatcgg gtaaggccca aaagtcaacc cggcagacac atctgcacgc
     2401 cagcctaata ccttcgtgac cccgccatta attgaaaatt tatcctctga cagtacacct
     2461 tccagatacc ctcctgcgac ataacctcca gcgacctggc cttttacatc tccatgtatt
     2521 tttgccgtat cacctactga cgggccgata tgtgatttgt ctaccgcttt gataatttca
     2581 tcgttagtga ctcctttacc actgagatca aaagccaggg aagcatcctg ctgtacgcga
     2641 ggcggcacaa gctcagacag cgcattattc tcaactaaag ccctttgccc taaaaagata
     2701 taacaaaacc caacaaaaac ctaatatgcc cccccccaga ccacccaact gagcagcata
     2761 aacaatatcg ctccacaaaa cttcaaaaat cccgctttga aaccatgcta acgctacaaa
     2821 cgttagttta gttattatgg ccgctaagat catgccaata aaacacaata agagaattga
     2881 actgcatata ataaacttac ctattaccac tcgagaagaa tatttcattt cttgagttcc
     2941 tcagatactt tatctgttac ttttccacct agctcagatc ctatagaacc gccaatatct
     3001 ccagcagttc caggaacatt gctcggtttc atgccttctc gaatgatatc gttacgattt
     3061 acttcagcat attttatcca tgttggatcg tacttaggat cccagccatt tgtcacccat
     3121 tttcctacag cattcgtaat cttatctgtc ccccaagtaa cacctttacc gataccatac
     3181 ccggcagcag aaccgatacc atttgaaatt gcaccagaca gaggatcttt gttgtcgatc
     3241 cagttaccca gcgcaccacc tgccgcattc cagcccacag tccccgccag gccattaccc
     3301 atgctgaaca cattcaccca gcctgcaatg gctgcgtttg ccggatctac agtaccatca
     3361 gccagataac tgataccagc attcgcactt gctcccagtc cccacatagc ctgagcacta
     3421 ccggggagta gtgccactgt acctgttgcc agagccagcc ctacagcatc cctctcattc
     3481 attgctttct ggcaggcggc accggaaggg ttttctgcac atgtcgccat gtcgatacca
     3541 tgctggcgta tccacgcagc ctgagcatct tcactaccgc caagtaaatt attctcaaca
     3601 gcattcttcc cgctctgtgc tcccactgct actgatgcag tgctgtttcc ggtaagaccg
     3661 ccggccagcc ctgctgaaac ggttgccagc gtacttatcg tctgcttctg ctcttcactg
     3721 aggtcagatt gcttcacgcc aggatacagc attccggcaa ttgctctggc tgccaattct
     3781 cctgttgcgg cgcctgctgc accggcagca acattatgac cctgcattgc cgctgctgca
     3841 ccgcccagaa tggcgtgagc aatggcgttt actgccgggt atttttctgt tgatttcagc
     3901 agatgcgcca gttccggtgc agaagctccg gccagagcac ccgctaaatt accacccacc
     3961 agtccctgaa gcgcggccgt cgcggcctgg ataccccgct gaatttcact accagtgcca
     4021 tacttcgcca tttcattcct gtaagcctgc gtgtcacgca gtttcgcaag atattcctga
     4081 cgttgtttct ctgttgcatt agcaggaagt gtttcgccgg ttgcttcctt cgcggccttc
     4141 aacgcattca gttctccctg tgtacgcgca atatccgcca cctgactgcc tatgtcactg
     4201 ataagcccca ctgtctgcag acgcctctgc tctttctcct tgtcaaatat cgggctgata
     4261 ctgtcattag cgtgcgcagg gtcacggctc aggttcgcca gattctgctt ctgattgtcc
     4321 ctgtcccgga tggtgattgt gccatctgcc actgcggcct gagtcgttcc ttccgcatgt
     4381 ccgctgtgac ctccggcgga tatcatgcca cccggcatgt taccctgaaa tttatccccg
     4441 aagctgccac caccgctcag actgattcca ctgtgactga ctttataatc cgcttcgttg
     4501 tgaaggtcac tgaaccccag cgttccggta tccaggtggt ttttatccgg tgtggcagtg
     4561 gaggcaatca ccgcaccatc cagttgggta tgtctgccca ctgtaatgtc gaaaccacca
     4621 tcaccggcaa acattccggt ttgttcagca acggagtcaa agcggctctt catcttatcc
     4681 cgggaggcag cgatgtaacc tgagccggtc atggagccaa aggtaaaact gccgccggca
     4741 gccacgctgg tctgtttact gtcgtactta ctggtgtcct gctggctgct tatcagcagg
     4801 tcgtggccca catcggcgat aatcctgttg ccgttgacct gagcaccgtt cagtaccgta
     4861 tcccgaccac tgttgatggt gacggtttta ccgctatcta ttgtggtttc agtccactca
     4921 gtaccgttac ctttctcgct gccttttgcc gcattaacgc tggcaaagac gctgataccg
     4981 gcaccgttac caccggcacc gatactgaca cccacgccac cgccactgct gctgttcctg
     5041 cccgttgttt tttgtgtgtt tgcagcgcca ctcaacagaa catcattcgc ggcatccagg
     5101 cttgtgttac caccggcctt aagctggctt cctgcaatca caatatctcc gcggttatcg
     5161 cccctgtttt tgccggttgc gacaacagac agattattcc cggcattcag cgtactgccg
     5221 gatactgtgt cactttcaga atgttgttgt gatttcgatt tctgggtggt gagcgacagg
     5281 ctgactcccg tcgcattcgg gtcaccggtt gcggtggcca ttgccgcagc ctgtccggcc
     5341 tgcacaccag acagcgctgt ctttgtggcc tgcaggactt tcagacggct gtcactgctc
     5401 tccttcgtct cctgtgcact ggtgaccgca ttattgatgg cactgcccac cgtgccggaa
     5461 agggcaaccg tcagcccgct tttcttctgc tcaaattttt cgtccacagt acgacggtca
     5521 tgccccgggt caaccaccac actgtcaccg gtaatgctga tatcccggtt cgcaatcaca
     5581 tccgaaccgc tgatatgagc ctgtttgccc gcggtaatac tgacattacc ggcagtggag
     5641 ccgatggtac tggcactctg actctgcgtt gtcccggcct cgcggcggtc gtgcgttgtc
     5701 ttactgctgc caatggtgaa accaataccg ccggtaccca tcagaccgga tttcttcgtt
     5761 tccttaaagc gccaggacgt atctgtactg gtggcagcaa gaacatcaac atggttaccc
     5821 gccgccagtg acacatcctg gtcagccacc acatccgaac cctctaccgt caggttatca
     5881 ccggcgttaa cggtcacgcg gttccccgac agcagggagc ctgcttcacg ggaggcactg
     5941 tcctcactga tggtgtgggt ggttttctta ctgagaaaac ctccgctttt tttcttcgtt
     6001 tccagatagt gatagtcact ttctgtcgcc gtggtcaggg caacatcacg accggcattc
     6061 acgctgatat tgccggttgc ggtaacggat gacgcaacag cggtgatatc ccgtcctgcg
     6121 ttgacggtgg tgtcaccacc gctggcgatt tccgttccct gctgacggac tgtctcgtta
     6181 atctctttct ttttcttcga cgtatagctg tcgcctgcac cggcagactc tgccaccagg
     6241 ttcacatcac gtccgccccg gatgaccacg ttattttccg cagccacacc ggcagcctga
     6301 ctggcaacat cacgaccagc aacaaggagg aggttatcgc ccgccgtcac cgtggacact
     6361 gctgcgtggc tttcatgact ttctgacctg ccgttgcgac tgtttttgct ttccctgact
     6421 gcattcagac tcaggtcgtt acctgcagaa agcagggcgc tgtgcccggc agaaacagag
     6481 gatgccgtga catccagatt atggcctgca gccatcgcca ggttaccgcc ggcgctgatg
     6541 ctgctgccct gtgaggtggt ggatgatgca ctgttgtcat cagtgtgcca gaaaccggac
     6601 tgacttttgc tcccgcttat caggtttgcg gcaatgttga tatcattacc cgcagacatt
     6661 ccaagggttc caccggacga gaccgttgcc ccggtaatat caatgttttt ccctgcatcc
     6721 agtgaaagtg aatcagtgcc tttaatggtc gcaaccggac cggtgtccgt accgctgaga
     6781 tgcacaccac catatcggct gtcactgccc gcattccatt gctgacgccg ggtgatattg
     6841 ctgatgttgc cactcacgct ttccagttgt acggttttac cgctgatgac tgagctgata
     6901 ttgctgatat ccccgatggc gctcaggtcc aggctaccgc ccgcgcttat cagccccgca
     6961 ttcaggttgt cgatatagcc ggtactgtcg agcgagaggc cgttttgtgc gttgatgctg
     7021 ccgccgctgt tggtgatatt gccacccgca agctgcacgt tgttcccgct gataacgctg
     7081 ccgttatgca gggtgatctc ttccggcgac agatacagtt tcgggaccat gactgtctgt
     7141 ccgttgatgg tggctgactc ccaccacagt atgctgccgt caagctgagc aatctgttca
     7201 gctgtcagcg ccacaccaaa ctctaatccc agtcctttct gttgtctggc cgcgttatcc
     7261 atcagatacc gcatctgttc cgtgtctgaa cccagtccgt tgatataacg tgaacccgtc
     7321 cggctcagca ccgcgttact gacataccgg gtatcaaaga ccgcatcccc caggaaacga
     7381 taatcttttt ccggtttcag cccgaggcgg tcaagaaaat acgatgagcc cagaaactgt
     7441 ttttcatcgg tatacgacgg agccgtttca cgtggcgcct gacccggctt cgctccagga
     7501 agctcataca gtccggcaaa caaatggctg tccacctgtc cgagaccatc cagtttcggg
     7561 tttaccgtaa tcagatacgg actgtccggg tccgtggacg gaaccaggta tccattgttg
     7621 ccggaaggca gtggccagtc atcactgata ccggtctgac cggtcagtgg cgaacctccg
     7681 gcaatatttt tcagggcacc tgccagttca tcgtgccatt gcggagagcc aaccaccacc
     7741 ggctcatact gctgcagcgc tgtctgtgtc agactgtctc cgccggtctg ctgacttaac
     7801 gtattcagta ccggtgcaga gaccaccgga ctgacactac ctgcatgtgc agtggttgtt
     7861 ccgttattga tactgctggt aaaacgggtc ttaacatccc cgcccgcctg aataacggaa
     7921 taatacgtct taccgggcgt gtaatctttt tcccggccat ccagtgaaaa tctgatggta
     7981 ttgttttcaa attccggtga cagcaggggc agtttatcca gagagcctgt ggcatagcta
     8041 ccgtaaaacg ttttcgggtc gtagcggtat accagatatt cattctctgt ccccgtctgc
     8101 cagctctgat tgcttaactc tctgcccgaa agtgcgatat ccccattcgc aaggataaat
     8161 gacgcccggt tttccagtcg ttcagcctca gcagaaagat tacgccctga cgcaatgcgg
     8221 cctgccggat tatcagcacc ggttactgtt gtgatgttct ggctgctgag aaagcgctgt
     8281 gtggcactgt cagcaaacgg cgcgtaataa taaagcgtat ccattgtgat attgcatgcc
     8341 ccgtgcccgt tgcagggcgt accgtgctga ttttcaactt cacgggtgaa atagccatag
     8401 ctgccgtcag gaagaaggga aaggggaata tcaaccagag catttcccat tccctgaatg
     8461 gatgaggggt tagtccgggt tgttgttgtg gcagaaaatc cctcccgctg gttcagaaga
     8521 tgcccggttc ttacaacaat atcgccctga tgcgtctcaa tattcccgga ggtattgata
     8581 atctctgtgt ttgcaccgcc ggaagcatcc ttctgtatcc acagactgtt gccggccagg
     8641 atatcaccat gctggttgtg cagacggtct gtaaacagct tcaggttatt ccccgcataa
     8701 atcagcgcac tgttcagcag ggtaccggcc acattcattg tcagactgcc tgccgtgccg
     8761 gtaaaaccac tgatggtgat atcactccgg ctgttcagac tcacatcgcc accggcctga
     8821 agtgaacccg gtgcgttaag gaaaagacgc tgtgcgctga aaacactgtt gcctttaccg
     8881 gcagtcagca ttccattgtt ggtgaatgcc tctccggcac cgagcaccat ggcatcaccc
     8941 tgcatcacac cgccgttggt gatggcattt tgcgacgtga cggaaagggt tttccctgcg
     9001 gccagggtac catgattcgt gagggcagca atcagtttca gtgtgacatc accggtggcc
     9061 accacctgac cctgaccact gaagtcctga gcgtcaagca gcaggttgcc tgcactgtac
     9121 agccgccctg taccattttg cagcagtgaa ctgcccttga cgccaagccc ggaggttccc
     9181 agcagggtac cgctgttgct gaatgtgtgg taattcacca gcaggtccgc cccctgaagc
     9241 gtaccggtat tattcagcgt ggttccttta acgtcggcac tgccggtggc aagtacgcgt
     9301 ccgccgttga cagtattcac cacatccagc agcagggtgg cagcctgtac cagtccgctg
     9361 ccggtgttcg ccagcacctg cgccgtcagc gtgaggttac tgccggagag gattttgccg
     9421 tcgttctgca gacggtcagt ggcgttcagg gaaaccccgc caccgccctg tatcgtgccc
     9481 tggttactca gggtcgcagt actgacattc agtgcattcc ggctcatcag aacaccaccg
     9541 gaacggttgt tcacgccacc ggaggcggcc agcgtcagcg tttcgccctg cagatgcccg
     9601 ccgtttgtga gttgtcctgc cgtgatggtg gtggcatttc cctgtaattg cccgtcgttt
     9661 gtgacactgt ctgccttcag cgtcagcaca cctgcactga gcagttttcc gctcgcgtga
     9721 ttgttcagcg tctgattcac cgtgagcgtg agagcatcca caccggtgat gtcacccgca
     9781 ctggtcagtg agttcgcctt cagagtcaga ttttttgcaa tccattgtcc gctgttgctt
     9841 aaattcagtg cactgagcgc catttcaccg ttcgaggtga ctttgctgcc cgcggcgctg
     9901 acaagctcac ccgtcagacg tgcagtcagg ctgtcagccg cctggatcgc cccgctgttt
     9961 gccagactgt ctgcggtgat cagcacccgt ttgccctgcc agtgtccgga actggtaata
    10021 ctgcctgcgg tgattgtcag atcgccgctg gtcagcagtg aacctccgtt attcatcagc
    10081 gcaggttgag gggataccat acgggcggca agcgtcagcg cggcgatccc ggtgaacgtg
    10141 ccactgttgg tgacactgtt ctggcgaatc gtgacatggt caccctggac agtgccgcgg
    10201 ttatccagtg aatttccatc aagggagagc gtgccggccg aaagcagact gccccggttg
    10261 tccgtggtgg ctgcgttcag cgtggtgtca ccctggctca tgatatcgcc ggtactggtc
    10321 aactgaccgg ttgccgaagc agtaaggtta ccggttgcca gcacggagcc actgttcgcc
    10381 cagttgtccg cctgcacggt gagattctgt ccctgcgtgg tccctgcggt atgcagtgtt
    10441 ttaccccgga gggagaggtc gcccgccgtc agccagcgtc cgttacggcc ctgtgagagg
    10501 gtgtcgcctg caagcgccag tgcaccggcg ccctgcaaca gaccatcacc atccagcgtg
    10561 gtcgccctga cgttcagcgt gtcagcgatg atttttcccg gattgctgag ggaggcagtg
    10621 tttaacgtta aaccattatc actggtgata agcccgctgt tgcggatgtc cggtatatcc
    10681 agcgtcaggt ctgcagcact gtacagcgtg ccgttctgct gattatcaag cctctgtgtg
    10741 ttaacggtga gtgaggcctc cccctgcaac agaccactgt tggtcagggt ctgtgactgt
    10801 gtattcaggg cggaaccaac aagcacaccg ctgctggtca gttccggcgc actgaggctg
    10861 agcgacgggg cgctgctttt cccgctgtgg gtgagctttt cactggcgtt caccgccatg
    10921 gtctgttgtg ctgcctgcgt acctgcaaga cgtgcatctc tggcgttgat gctgagattt
    10981 ttaccgctct gaagctgtgc gcccgctgcg gtactcagtt tgtctgcctg aaccaggagg
    11041 gtgtcaccgg cactgttttc cccgtccagc gccactgttg tcacattcag cgtcatcgca
    11101 gcatcgctgt gggtgaccga ttttttaccg gagctcagcg cctgcgcact gaccgtcagc
    11161 cctttgccgc cggacagcac accgttctgt gtcacatcct gcgccttcag caccagtaca
    11221 tcatcgctca ccagcgaacc tgtactggtc agtttcccac tggccgtgat atccactttg
    11281 cccttcgcgc cagtgcggcc gctctgggta aagtcgcggg tattcacggt caggggaccg
    11341 ccactgagca gggagccact gttgctgagc gttgtactgc cgagcgtcag ggaagccccc
    11401 tgaacagcac cactgttatt cagcgtgccg gcatcgagtc ccgcatgacc tttcgccagc
    11461 aatattccgt cctgtgtcag cgtggtggcg ctggccgtga gattccgccc ggcggttatc
    11521 tgtccctgtg ttgtcagcgt gtcactggcg acagtcacaa tatcgcgggc cgcgttaatc
    11581 tggctggcgg tatcctgtgt gatgtttttc gcggcaagcg ttacatcccg gccggcagtc
    11641 agtttttcat tctgttgagt gattctgccg ccggcggtca ggctgaggtc cttgtcgctg
    11701 ttaagcgttc cattgctgag aacgatatcg ctccggctgc tgacgctcag gttacctccc
    11761 gctttatggt cgccggttaa ggtgacgccc tgaccttttg cagtgacggc ccccgtggcg
    11821 agactgttgt tgacagtcag tctgccgctg gcatccaggg tgatatcgcc atcgcgggca
    11881 taaaggttac caagattaac cccgacacca ctttcagtgg aggtcagatg aatacgcctg
    11941 gcgtacattc caccgagcgc gccggtatca acggcaactt tcggcacatc accttcgccc
    12001 ttcagtgcac tgacacgacc atctgcagtt atccggttag cgcctgcagt gacagttaaa
    12061 tccttcgcat gaagcgcggc attcacttcc gttgcacggg caataatgga tacggcatcg
    12121 ctccgggtgc cgtccaggcc cgcgccattg atggtgatac tgccttcagt cacctccagc
    12181 gcctgcaggc tgccgtcggc attcatcaca ggtctgcccg tggtgagcgt cgcgtgcggc
    12241 gtgttgataa aaccacagcc gtcacaggtg ataccatacg ggttggcaac catcacattc
    12301 gccgctttgc cggccacttc cgtatagccc tgcagcagtg aacggttacc gccggtcact
    12361 tcgttgatga tacccttcgc ttccccgccc gctttcaggt tcgggttatt ctgtatcagt
    12421 ccaccaagct gcgtcggatt aagcttaccg gtggcattat tgagaatcag cccttccttc
    12481 ccgacgttgt aatccgtaaa ccggttatgc gaaatcccgg ccccgttcgg cgtggcaatg
    12541 ttcacgaccg gcacaccatt tgccgcttta tccattccgg ccccgttttg tggggtgatg
    12601 acggccccca cagccggtaa caacggctgc ccggcgataa tcgcactgac caggtaactc
    12661 agcaggcggt aagtgaagtg aacgggaggc tgattcatgg taataatccc ttaaaacgcg
    12721 acggcaacgc gccagtaaac cgtgagatgg tcaggggcaa gccagtccgg gtaaaccaga
    12781 ggcagcccgg cagtgaacga accggaaaca tagtcactgg tggtgctgag cccggcagca
    12841 ccaccccaca gcgtgccgga cgagtacggg tcatctctgt cagagtgcag ccagccgccg
    12901 tccagtgcag ccactgcacg gacagttccc acatatggca gggagaacag agaccaggac
    12961 agctcatttc gcagataacc gccgttatta ccggagatat actgctcctt aaagccacgc
    13021 actgaactct cacccccgag gctcagttgt tccacaccat gaagacggtc cggtgaccac
    13081 tgggcataag cgctggtcag ccaccacacc ctgtccgtga cggggcgctg aaaactggca
    13141 ctcaccgacc atttccggaa ctgatttacg ggcaggtctc cccttttccc gtggtcgctt
    13201 tctgcgccga accagggcat cccccgtgtg aataccggat tcagtgttcc gacacccccc
    13261 agaaacttgt gtgtgtgatt cagcccgaca gaaaatgaag tgagtttacg gctgctgccc
    13321 tgaagcagaa catcatccag ataattgtga ataatgcggt gctgcagacc tccggtcagt
    13381 gctgtcttca tgtccccgtt acggaacagg acatgcgaca gtcccagccg gtgagtctgc
    13441 aggtctcccg tggaacgcca ccgccagccc cggttatcaa tggtgctgag gtagtcactc
    13501 catgaatacg tataatccac cagggtatag ccatacggca gactgacacc ggcggcaaaa
    13561 ttcctcgcat catgtgacac cgaaaagtca ctgctccgtc ccccgctgac aaaccagttg
    13621 tcagccagcc ccagcggatt attaaaggaa aggacaccat ttaactgccc cgtaccggta
    13681 ttcttctgcc cgctgttgtc gatacccacg ctccctgtga cgggccattc cggcaatgcc
    13741 gtcagtgtca ccaccgacca tccctcacgg tcaccgggcg atatttcaat ctgtaccggc
    13801 tccgtacgca gacgattaat ctgctccatc ccctgctcaa tatcccgcag gttcagaacc
    13861 ttcccctcca ttcccgggaa aaccatcttc agggtgcggg caggaaggtc agcgccttcc
    13921 gcccggattt gctgcagcct gccctccatg accgttatgt gcagtacgcc ccctgaaagg
    13981 tcctgctctg tcagaaaggc ccggctggtg atatatcccc ggcgtatata gccgtccgtc
    14041 acggcatccg tgaccgcagt caggccggtg atattcagac actgattcac ccacggtgcc
    14101 accagtctgt cggtttctgc agacgtcagt cgggtggccc cactgacaac aatgcgtgac
    14161 accgtaaagc aggggccttc agtaccggca gacgtttccg gagacggcgt gatggtcagc
    14221 ggcgcactgc gctccagcgc atcacgctga cgctggtttt catccagcaa ctgctgctgt
    14281 tgctgctgaa ttgcactgcg gtcacccgga gacagcatgg ctgccggaga cgaaaatgcg
    14341 gggataaaaa aaccagaaaa aacggagagg gagagatagc atatccacgg agagccgcgc
    14401 cggaacatat cacaacaggg gacagacaac gcacgggata aaagggacgt tttcgtcaga
    14461 atgttgttct gccggcgctg catgttctcc ccgtattctg gcctgtgtga tgccagtgta
    14521 aattcatgtc aaaaagtgtc agcgagaaaa tttacacctg aaaaagtctg tcatgaaaca
    14581 gttttatgac aagacatcaa aaaaatgtca aaaacgtgat ctttcttcag cttttcagaa
    14641 tattgagtac caggaagtca atatcaattc tgggacacat atgcatttac cattaaaacc
    14701 gggcacaaca agctgttcag tctcaatcca taccccgtgt tttatcaata tcagaataag
    14761 aatatctgcg gataaataaa aaaggaatgt cgagggagct taccgcagag ggctctccac
    14821 ctcacaaacc atttacaaaa atgataccgg catatctttg gtccctgtca caaccagaca
    14881 ttgacaatcc aatataaccc caaatatatt tcgggtaaga agtctgggat ttatacagat
    14941 atactgtctg ttattactat ttaattatat aattgtattg catacataag aggcactaat
    15001 gaaacgttat ataaaatggt ttgccatcac aatttttatc agtatgttga gtgcctgtgc
    15061 ccgtacggcc ccagtgcaac agataagcac cactgtcagt gtgggtcata ctcaggagca
    15121 ggttaaaaat gccattttga aagcaggtgc gcagcgcaag tggattatga cgcaagtgtc
    15181 ccctggagtt attaaagctc gctatcaaac acgaaatcac gttgcagagg ttcgtattac
    15241 atatacagct acctactata acatcaaata tgacagtagc ctgaatctgc aggcttctga
    15301 tggaaaaatt cataaaaact ataaccgctg ggtgcgtaac ctagataaag atatacaggt
    15361 taacttatct acaggagcaa cgttatagtg atgctgatat cgggctggaa tggcccgata
    15421 gtatttaact ttctctataa gaatggttat atatgtacga acaggacacg ctcagcgcac
    15481 tggatgccat caccgaagca cagcggattg cctttgctcc tatgcttttc caggcagctt
    15541 tgtgcctgcg caatgcagga atattagatt atttggatca acagggtaaa cagggagctc
    15601 cccttaatgc gattactgag cacacagtcc tgaatgaata tgccgtgggt gtgttactgg
    15661 atatgggctt aagcgggcga attattacct gtaaagaggg aatttattac ctggcgaaaa
    15721 tcggtcatta cctcctgcat gacactatga ctcgcgtgaa tatggatttc acccaggatg
    15781 tctgttacca ggggctcttc ttccttgccg attcactcaa tgaaggaaaa ccttccgggt
    15841 taaaagtatt tggcgactgg ccgacgatat atccggcact ttcgcaatta ccagacgccg
    15901 cacgtgatag ctggtttgcg tttgatcatt actattcaga tggcgcattt aacgccgcac
    15961 tgccttatgt attcgccaac aaccccacaa ccttgtacga tgtgggtggc aatacgggta
    16021 agtgggcttt gcgctgctgc aaattcaatg aaaacatcgc cgttacgtta ttagatctgc
    16081 cacaacaaat cgtactggcg aaagaaaata tcgcgaatgc aggtttttct gatcgcatag
    16141 atttccatgc ggtggatatg ctaagcgatg ccccattacc aggcgaggct gatatctggt
    16201 ggatgagcca atttttagac tgtttttcac ccgaacaaat aattagcata ctcagcaaaa
    16261 ttgccagtgt aatgaagcct ggcgcaaaac tttgcatcat ggagttgttc tgggatgcac
    16321 agcggtttga agcgacatcg tttagcctga atgcatcctc actttatttt acctgcatgg
    16381 caaacggtaa cagccgtttc tatagtgcag agaaatttta cgactatctg aataaagcag
    16441 gattccaggt agcagagcgt cacgataatt taggcgttgg gcatacactt ttgatatgcc
    16501 aaaagaaata atcgtcactt aataattttg gtttaccgcg ggcgcgcgtt gatgaaattt
    16561 gcattaaata ttaccaactg gcaggcactg gcgcctggac ttagcgatgt gcagcaatgg
    16621 caagcatggt cgcgccagcc gtgggcgatt gaccctgccg cgccgttggc aaaattaagt
    16681 gaactgccaa tgatgaccgc tcgtcgtctc agttcaggca gcaaactggc tgttgaatgc
    16741 ggtctgacaa tgctacgccg ccatcagcca gacgctgttt tgtataccag tcgtcatggc
    16801 gaactggagc gcaattaccg cattgttcat gcactggcaa ccgaacaggc gctttctccg
    16861 acggacttcg cgctttccgt acacaactct tccgtgggta acctgaccat tgtggccaaa
    16921 cagccgatcg tttcgtcatc gctttcggct ggtcgcgaca gtttccagca aggtttgtgt
    16981 gaagtgctta gcctgctaca agcgggatac cagcgcgtgt tgatggtcga ttttgatggc
    17041 tttctacctg agttttatca cccgcaactg cccgcagaaa tgcctacctg gccgtacgcc
    17101 gtcgcgctgg tgattgaagc tggcgatgac tggcaatgtg aaacgcagcc agccattgcg
    17161 gtaaatgaaa caacactgcc gcaaagcatc ttgtttttac agcactattt acaaaatgca
    17221 gatgcttttt cactgcctgg cgagcgcgtg caatggcgct ggagccgcag atgagtaagc
    17281 tgatgtcgcg cctgggatgg gcatggcgac tggtcatgac cgggttatgt tttgtcctgt
    17341 ttggtctcgg cggcctgctg ctttcagtcg tgtggtttaa catattgtta gttttggtgt
    17401 gggatacttc ccgccgccgt cgtttggcac gtcgcagcat tgccgccagt tttcgcctgt
    17461 ttttaactgt cgcgaaaggg cttggcgtgc tggattatcg tattgatggc gctgaaattt
    17521 tacgtcagga acgcggttgc ctcgtcgtag ccaatcatcc cacgctgatc gattacgtgc
    17581 tgctggcatc agtcatgcca gaaactgact gcctggtgaa aagcgcgctg ttaaaaaatc
    17641 cttttcttgg cggcgtcgtg cgagcggcag attatttaat caacagcgaa gcagaaaccc
    17701 tgttaccccg ctgccaacag cgactggcac agggtgacac tatcctgatt ttccccgaag
    17761 gcacacgaac gaagcccggt gaaaaaatga cgttacagcg cggtgcagcg aatattgccg
    17821 tgcgctgcac cagcgattta cgcatcgtga cgatcggctg tagcgagcat ctgctggata
    17881 aacaaagtaa gtggtatgac gtcccgcctg ccaggccgtt ttttacggtt gaagtccgtg
    17941 ggcgggtaga aatcaataaa ttttacgatg caacttcaca agagccgacg ctggctgcaa
    18001 gacagttaaa ccggcatctt ttgcttcaat tacagcaagg ttgtttacct ctatcaggaa
    18061 taaataatgc aagcgcttta tctggaaatt aaaaacctca tcatcagcac cctcaatctg
    18121 gacgacttga ctcctgatga tattgatatc aatgcgccac tgtttggcga cggtttaggg
    18181 ctggactcta tcgatgcact ggaactggga ctggcagtaa aaaatgagta cggcatcgtg
    18241 ctctctgcgg aaagcgaaga aatgcgtcag cactttttct ccgtcgccac tctggcctct
    18301 ttcattgctg cgcaacgtgc ctgaggaaaa ccatcatgac agaccaacaa accatttatc
    18361 aggaagtctc agccctgctg gttaagctgt ttgaaatcga tccgcaggac atcaaacctg
    18421 aagcgcgact gtacgaagat ctggaactgg acagcatcga cgccgttgac atgattgtgc
    18481 acctgcaaaa gaaaaccggt aagaaaatca agccggaaga gtttaaagca gtgcgtacag
    18541 tccaggatgt tgtcgaggct gtagaacgcc tgctacaaga aggctaattt gatgtcgggc
    18601 attcgctcgc taccgatgat taagttgctg accggattat tgctgctcgt ctggcctttt
    18661 ttaatctggc taggtttggt gcataacagc ctgcactggc tgttgccgct gatggcgctg
    18721 ttgcttttat tgcgcctgcg ccagccccgt cgccagacgg ggccgctcca ggtcgtcaca
    18781 aaaatcgttg ctgttgtggg cattgtattg tgtgtctcca gttttctgtt gaaaacgtac
    18841 cagttactgc tgttttaccc ggtagtggtt aacgcagtca tgctggcggt ttttggcggt
    18901 tcgctgcggt cgacaatgcc gatagttgag cggctggcac gtttgcagga gccagacctc
    18961 ccggaaaagg ccgtgcgata tacccgccgc gtaacgcagg tctggtgcct gttttttatt
    19021 ttcaatggca gtattgcgtt atttaccacc ttgtatggca acatgccctt gtggacagcc
    19081 tggaacggta tcattgctta ccttctgata ggtttcctga ctgcagggga atggctgata
    19141 cgtcgtaaga tgatcaaacg gaaaacacca tgaatcagac tctctcactt tcacagtggt
    19201 tgacagcctc tcgccctgta acaactcctg ttgcctggct tggggaatat acctggacac
    19261 tggggcatct gcggcatgat gtagcgcttt tgatcgacca tttacgcgac cagccaggca
    19321 atcgctgggc actttgtttt gaaaacagct atctttttat tgtagcgttg ttggccacgc
    19381 tacactcagg taaaatcccg gtaatcccgg gccataatcg cgccgctttg ttaaatgtac
    19441 aacgctcctt gtttgattgc gtcctgagtg ataaaaggct gggctggaac ggtccacagt
    19501 ttgttgtaag cacatctcac cagatgacga ccagcacgat agcctttgat gatattgctt
    19561 ctgacgcgtt tattgaactt ttcacatcgg gttcaaccgg acaaccaaaa caaattgcaa
    19621 aacctgttat cagccttgat caagaagcaa acatgctggc aactcacttt gcagaccgac
    19681 tactgggctg tcgttttgta gcatccgtga tgcctcagca cctgtatggg ctaactttcc
    19741 gtattttttt acccatggca cttggcctgc cattgcatgc cgcaatgctc tggtattctg
    19801 agcaacttgc cgcattaagc catacctacc gctatgcctt tgtcagcagc ccggcgttcc
    19861 ttaagcgact cgaccttcat ctgacgcctc ccccagtgga aatgattctc tctgccggcg
    19921 gtatgctacc atggcaggat gtagagaaaa cttccacctg gctgaatata tggcccgatg
    19981 aaatctatgg tagtaccgaa acaggtattc tcgcatggcg ttaccgtcaa caggataata
    20041 tcccctggtt tactttttct gatgttcatc tttcacagga aagtgagggg gttcgtgtct
    20101 tttcgccact aatccctgct gaaggattgg tgcttgacga tatgttgcaa ttcaatgaaa
    20161 acggtcagtt ccacctgatc ggacgacgtg gtcgagtggt aaaaatagag gagaagcgga
    20221 tatcactggt tgaaatcgaa caacgacttc tggcacttga cggtattatg gatgtcgctg
    20281 caataccatt aatacgcaat ggccgccagg ctatcggtgt cgtggttgta ccaaataaag
    20341 aggcccgtca aatatggcaa tgctccggag ggaaaactct ggagttgtcc tggcgcaaag
    20401 cactattacc atggcttgaa cctgttgcag tacctcgtta ctggcgcatt attgatgaaa
    20461 taccagtaaa cagtatgaac aagcgtgtct atgcgcaatt acaggagtta tttcatgata
    20521 cgccatgaaa ttgagcgcca tctggttcaa ccgcaggaga tggaaatcgt tctatacctt
    20581 gacccgatgc tctactggtt taatggtcat tttgccgtac agcctctgtt accaggcgtg
    20641 gcccagttgg actgggtaat gcattatgct accacgctgc ttgctccggg ctggcgtttt
    20701 cgtagtatcc agaatgttaa atttctggcg ccacttatac ctgagacgac tgttaccctg
    20761 caattaacat ggcaggaaac aagtcaggta ctcactttct gctatcagcg tcacgacggt
    20821 gacgcccgcc acaccgccag tagcgggaag atccgtctat gtcggtaaac ttttctccct
    20881 gcgtgttgat cccctgctac aaccacggcg cgatgatgcc gggcgtgctg gcgcgtctta
    20941 agccatttaa tctgccctgt attgtggtgg atgacggcag cgatgccgcc acgcaacagc
    21001 aactggataa tctggttagc gaacagcctg gcgtgacctt aattcgcctg gcagaaaacg
    21061 caggtaaagg cgcagcggtg atgcgtggct tgcaggcagc ggcagacgca gggttcagcc
    21121 atgcggtgca ggtggatgct gacggtcaac atgcgattga agatatcccc aaactgctgg
    21181 cactcgctga acaacaccct gtggcgctga tctccggtca gccaatttac gatgactcca
    21241 ttccccgctc acggctgtac gggcgctggg tcacccacgt ctgggtatgg atcgaaacgc
    21301 tctccctgca actgaaagac agcatgtgcg gttttcgcgt ttatccggtt gcgccgacgc
    21361 tgcaactggc aaaacgcgcc accatcggca agcggatgga tttcgacacc gaagtgatgg
    21421 tgcgcctcta ctggcaggga aataccagct atttcgtgcc gacccgcgtc acctatccac
    21481 tggacgggct ttcgcatttt gatgccctga aagataacgt ccgcatctcg ctcatgcaca
    21541 cgcgtctgtt tttcggcatg ttgccgcgta ttccttcact gctgatgtgc cactcttcca
    21601 gccactgggc gcggcagagt gaagttaaag ggttatgggg aatgcgcctg atgctgctgg
    21661 tctggcgtct gctgggaaga acggcgttta ccgcgctgct ttacccggtg gttggcgtct
    21721 actggctcac cgctgctcgt gcgcgtaaag cgtcgcaaga ctggctcgcc cgtgtgcgcc
    21781 aacaacaacc acaggcagca aaaatcaaca gctatcagca ctttctacgt ttcggtaatg
    21841 ccatgctcga caaaatcgcc agctggcgcg gcgagctaca actggggcac gatgtgctgt
    21901 ttgcgccagg cgcagaagcg gcgcttgacg tccgcgatcc gcgcggcaaa ttgctgctgg
    21961 cctcgcatct tggcgatgtg gaagtgtgcc gggcgctggc aaaaattcag ggctacaaaa
    22021 ccattaacgc gctggtgttt agcgaaaacg cccaacgctt taaacagata atgcaggaga
    22081 tggctcctca ggcaggcatt aacctgatgc cggtaacaga tatcggcccg gaaaccgcca
    22141 tcctgctgaa agagaagctg gataacggcg aatgggtggc gattgtcggt gaccgcatcg
    22201 ccgtcacccc gcaacgcggc ggcgactggc gcgtctgctg gagtccgttt atgggccagc
    22261 ctgcgccttt cccacagggg ccgtttattc tcgcgtctat tttgcgctgc ccggtgaatc
    22321 tgattttcgc cctgcgccag cgcggcaagc tgcatattca ctgcgaaacc tttgccgacc
    22381 cgctgctgtt gccgcgtggc gaacgccaac aggcgctgca aaacgctatc gaccactacg
    22441 ccgcgcgtct ggaacattac gcgctccagt cgcccctcga ctggtttaat tttttcgatt
    22501 tctggcaact gccggaaatt caggacaagg agtaaagggt gcttaacgat ccccgattta
    22561 ccgctgaagt cgagctgacc attccctttc acgacgtcga tatgatgggc gtggcctggc
    22621 acggtaacta ttttcgctac tttgaagtgg cccgcgaggc gctgctcaac cagttcaatt
    22681 acggctatcg gcagatgaaa gagtcaggtt atttgtggcc agtggtcgat gcgcgggtga
    22741 aatatcgcca cgccctcacc tttgagcaac gcatccgcgt gcgtgcgcat atcgaagagt
    22801 tcgaaaaccg tctgcggatt ggctatcaga ttttcgatgc cgaaacgggc aaacgcgcca
    22861 ccaccggata caccattcag gtcgcagtag aggagcaaag ccgtgaactg tgctttgtca
    22921 gccccgatat tctgtttgaa cgcatgggag tcaaaccatg aaatttttac cgctgctggt
    22981 gctgctgatt agcccgtttg tgagcgccct gaccctggac gatcttcagc aacgctttac
    23041 cgaacaaccg gtgatccgcg cccatttcga tcaaacccgg acgattaaag atctgccgca
    23101 gccgctgcga tctcagggtc agatgttgat cgcccgcgac caggggttat tgtgggatca
    23161 aacctcaccg ttccccatgc aactattact ggacgataaa cgcatggtgc aggtgatcaa
    23221 cggtcagccg cagcaaatca tcacggcaga aaacaacccg cagatgttcc agtttaacca
    23281 cctgctgcgc gcgctgttcc aggccgatcg caaagtgctg gaacaaaact tccgcgtcga
    23341 atttgccgac aaaggcgaag gccgctggac gctgcgcctg acgccgacca ccacgccgct
    23401 ggacaaaatt ttcaacacca tcgatctcgc cgggcaaacc tatctggaga gcattcagct
    23461 taatgataaa cagggcgatc gcaccgatat cgctcttacc caacatcaac tgacaccagc
    23521 gcaactgacc gatgacgaac gccaacgctt tgccacccag taaacgcccc gcgctgttat
    23581 gggggctagt ctgcctggtc atggcggcgg cgttgctgat cctgctgcca caatcacgac
    23641 tgaacagtag cgtgctggct atgttaccca aacaggcgat gggcgatatt cctccggcgc
    23701 tgaatgacgg ctttatgcag cgtctggacc gccaactggt gtggctggtc agccccggta
    23761 aagaggctaa tcccagggtg gctcaggagt ggctgacgct gctgcaaaaa tccgctgcgc
    23821 tcggcgacat taaagggcca atggatgccg ccagccagca ggcgtgggga gcgtttttct
    23881 ggcagcaccg caacggcctg attgaccccg acacccgcgc ccgcctgcaa aatggcggcg
    23941 aagcgcaggc gaagtggatc ctctcccagc tttattccgc gttctccggg gtaagcggca
    24001 aggagctgca aaacgatccg ctgatgttaa tgcgcggctc gcagctggcg atggcgaaaa
    24061 atggccagcg tttacgacta atggacagct ggctggtgac gcaggatccc cagggcaatt
    24121 actggtatct gctgcacggc gaactggcgg gatcgtcgtt tgatatgcag caaacccacc
    24181 agctcatcac gaccctgaat acgctggaaa aggctctgaa aacgcgttac ccgcaggcgc
    24241 agttgctctc acgcggcacg gtgttttaca gcgattacgc cagccaacag gcgaagcagg
    24301 atatctccac cctgggcgtg gcaacgctgc tgggggtgat attgctgatt gtggcggtgt
    24361 tccgctcttt gcgcccgttg ctgctttgcg tgatttccat cggcatcggc gcgctggcgg
    24421 gaacggccgc cacattgttg attttcggtg aattacacct gatgacgctg gtgatgagca
    24481 tgagcgttat cggcatttcc gctgactaca cgctctatta tctcaccgag cggatggtgc
    24541 acggcaacga cgtttcgccg tggcaaagcc tggcgaaagt acgcaatgcc ctgctgctgg
    24601 cgctgctcac aaccgtggcg gcatatctga ttatgatgct cgcccccttc cccggcattc
    24661 gccagatggc gatttttgcc gccgtcgggt tgagcgcctc ttgtctgacc gtcctgttct
    24721 ggcatccgtg gctgtgccgt ggtctgccgg tgcgcccggt tccggcgatg gcgctgatgc
    24781 tacgctggct ggcagcgtgg cggcgcaata aaaaactgtc gcggggtctg cccgttgcgc
    24841 tggcgctgtt ttcgctggcg gggatgtcaa tgctgcgcgt cgatgacgat atctcgcagt
    24901 tacaggcgct gccgcagcac attctggcgc aggagaaagc cattaccgcc ctgaccggac
    24961 aaagcgtcga tcaaaaatgg tttgtagttt acggcgactc cccacagcaa acattgcggc
    25021 gactggagaa atataccgcc tcacttgagt atgcgaaaaa agaggggctt atcagcaact
    25081 accgcactat tccgctgaac tccctggcgc ggcaggagga agacttagac ctgctgaaaa
    25141 cggctgcccc gacggtaaca aaagcactgc aaaatgcagg gctgacggcg gtgaaaccag
    25201 atctcaacgc catgccggtg aaggtcgatg aatggctggc aagccccgcc agtgaaggct
    25261 ggcgtctgct gtggctgacg ctggaaaacg gcgaaagcgg tgtactggtg ccagttgaag
    25321 gggttaaaag tagcgcattg ttgcaggaaa tcgccacata ttacccttgc ggcattgcct
    25381 gggttgatcg caaaagcacc tttgatgaat tgttcgcact ttaccgctac gtcttaaccg
    25441 gcttgttgct ggtggcgctg gcagtgattg cctgcggcgc agtggcccgt ctcggctggc
    25501 gcaaagggct tatcagcctg gtgccttcgg tgctttcgct gggctgtggt ctggcggtgc
    25561 tggcgatgag cgggcaggcg gtgaatctct tttcgctgct ggcgctggtg ctggtgcttg
    25621 gcatcggtat caactacacg ctgtttttca gtaatccgcg cggtacaccg ttaacttcgc
    25681 tactggcgat cgcgctggca atgctcacca ccttgctgac gctgggaatg ctggtattca
    25741 gcgccaccca ggccatcagc agttttggca ttgtgctggt gagcggtatt ttcaccgcct
    25801 tcctgctttc gccgctggct atgcccgata aaaagagaac aaaaaaatga tcaaatccac
    25861 attctggcga gcgttcgccc tgaccgctac gcttatcctc accggctgta gccactcgca
    25921 accagaacag gaaggccgcc cgcaggcgtg gctgcaacct ggcacgcgca tcacgctgcc
    25981 ggcaccaggg atttcacccg cagtcaattc gcagcaactg ttgaccggca gcttcaacgg
    26041 caaaacccag tcgctgttag tgatgcttaa cgccgacgat cagaaaatca cccttgccgg
    26101 gctgtcgtcg gtcggcattc gcctgtttct ggtgacctac gatgcgcaag ggctacgcgc
    26161 cgagcaatcc atcgtcgtcc cacagttacc gcccgcaagt caggtactgg ctgacgtgat
    26221 gctcagccac tggccgatta gcgcctggca accgcaactt cccgcaggat ggacacttcg
    26281 cgacaacggt gacaaacgcg agctgcgtaa cgccagcggc aaactggtca cggaaatcac
    26341 ctatctgaat cgccagggaa aacgcgtgcc aatcagcatt gagcagcatg tctttaaata
    26401 ccacatcacc attcaatact taggtgactg atatgattta tatttccgcc gtcggcatga
    26461 tcaacgccct gggaaataac cttgacgaaa tcgcagccaa cctggctcgc ggtgtcgccc
    26521 ccggcatgcg cccacgcgca ggctggttgc aggggcatcc gcaggcggtc ctggctggcg
    26581 tggatggcga gcttccgctt atcccggaaa aattcgctgc gcatcgctcg cgcaacaatc
    26641 agatcctgct ggcggcgctg gcgcagctcc agccgcaggt tgatgacgct atcgcgaaat
    26701 atggctgcga gcgcatcgcg attgtgcttg gcaccagcac ctccggcctg catgaaggcg
    26761 acacgcacgt taacctgcac acccacgggc agccgagcac cacgtggcac tatgcacaac
    26821 aagaactcgg cgatccgtcg cgttttctca gccactggct ggcgctcgac ggcccggcct
    26881 ataccctttc aaccgcctgc tcttccagcg ccagagcgat aatcagcggg cgcaggctta
    26941 tcgaagcggg gctggtcgat gccgccattg tgggtggcgc agacaccctg agccggatgc
    27001 cgattaacgg ttttcacagc ctcgaatcgc tgtcgccaac cttatgtcag ccgtttggtc
    27061 gtgaccgcgc aggtatcacc attggtgaag gggcggggct aatgctgctg acccgggaac
    27121 cgcagcccat cgcgttgctg ggcgttggtg aatcgagcga cgcttaccat atttcagccc
    27181 cgcatccgca gggcgaagga gcgatccgcg ctattaatca ggcgctgacc gatgcgcagc
    27241 ttacgccaga tgacgtcggc tatatcaacc tgcacggcac cgccacacaa ctcaacgatc
    27301 agattgaatc aatggtggtt aacgccctgt ttggcgaacg cgtaccgtgc agctccacca
    27361 aacatttgac cggacacacg ctgggcgcag cgggcattac cgaggcggca attagtatgt
    27421 tgattttaca gcgtgattta ccgctgcccg ctcaggactt cagcctgtcg ccgcgcgatc
    27481 ccacgctgcc accgtgtggc attatcgaaa aaccgcagcc gctggcacgt ccggtgatcc
    27541 tgtccaattc cttcgccttt ggcggcaata acgccagcat tctgctcggg agggtttcgt
    27601 gagccactat ttatcccccg gcgcttatct gccccacgac gcgcccatgt tactgctgga
    27661 agacgtggtg agcgtcagcg atgacagcgc ggtgtgccgc gtgacggttt cgcccagtgg
    27721 cgtactggca ccgtttctcg acccggacgg caatctccct ggctggtttg cccttgaact
    27781 gatggcgcaa accgtcggcg tctggtctgg ctggcatcgc caccagcagg gcaaaaacag
    27841 tattgagtta gggatgatac tgggcgcgcg tgaattgctt tgcgccgctg gcattctgcc
    27901 cgcaggccag acgctgacca ttaccgtcaa actgctgatg caggacgatc gcttcggcag
    27961 ttttgagtgc agcatcaacg acggcgaggc gacgggccgc attaacacct tccagccgac
    28021 cgcggaagaa cttaccaccc tgtttcaaca aggagcgtcc tgatgagtcg ttcagttctg
    28081 gttactggtg ccagcaaagg cattggtcgc gccatcgcct gccaactggc ggcagacggc
    28141 tttaacattg gcgttcacta tcaccgtgac gccacaggcg ctcaggaaac gctgaatgcc
    28201 attgtcgcca acgggggtaa cgggcgtttg ctcagtttcg acgtcgcaaa ccgcgaacag
    28261 tgtcgggaag tgctggagca tgagatcgcg caacacggtg cctggtacgg cgtggtcagt
    28321 aacgccggga tcgcccgcga tgccgctttt ccggctttaa gcgatgacga ctgggatgcg
    28381 gtgatccaca ccaacctcga cagtttctac aacgtcattc agccgtgcat tatgccaatg
    28441 atcggcgcgc gccagggtgg gcgcatcatc acgctgtcct cggtttccgg cgtaatgggc
    28501 aatcgcggcc aggtgaatta cagcgcagcc aaagccggaa ttatcggcgc aacaaaagcg
    28561 ctggctatcg aactcgcgaa gcgcaaaatc accgtcaact gcatcgcgcc ggggcttatt
    28621 gataccggga tgatcgaaat ggaagaaagc gctctgaaag aggcaatgtc gatgatcccc
    28681 atgaaacgta tgggccaggc ggaagaagtt gccgggcttg ccagttattt aatgtctgat
    28741 attgcgggtt acgtcacccg ccaggttatt tccatcaacg gagggatgtt atgacacgtc
    28801 gcgtagtgat tacaggaatg ggcggcgtga ctgcctttgg tgaaaactgg caggacgttt
    28861 ctgcacgctt actggcgtat gaaaatgcgg tgcgcaaaat gccggagtgg caggtctacg
    28921 atgggctgca caccctgctt ggcgcgcctg ttgatgattt cacgctaccg gagcactaca
    28981 cccgcaagcg tatccgtgcg atgggccgcg tgtcgcaaat gtcgacccgt gccagcgagc
    29041 tggcgttaga gcaggcaggg ttgattggcg atccgatatt aaccagcggc gaaacgggca
    29101 ttgcgtacgg ttcatcgacc ggcagtaccg gcccggtaag cgagttcgcc accatgctga
    29161 cggaaaagca caccaataac atcactggca ccacctatgt gcagatgatg ccgcacacca
    29221 ccgccgttaa tactggcctc tttttcggcc tgcgcgggcg ggtgatccca acctccagcg
    29281 cctgtacttc cggcagccag gcaatcggtt atgcgtggga agccattcgc cacggttatc
    29341 aaacggtgat ggttgctggt ggtgccgaag agttatgtcc ttcagaagca gcggtatttg
    29401 atacgctttt cgccaccagc cagcacaatg atgcgccgaa aaccacacct tcaccgttcg
    29461 atgaaaaccg cgatgggctg gtgattggtg aaggcgcagg tacgctcatt ctcgaagaac
    29521 tggagcacgc caaagcgcgc ggcgcgacca tttacggtga aatcgtcggc tttgcgacga
    29581 actgcgacgc ggcacatatt acccagccgc agcgcgaaac catgcagtat tgtatggagc
    29641 aatcgctgaa aatcgccggg ttaagcgcac aagatatcgg ctatatttca gcgcatggta
    29701 cggcgaccga tcgcggtgat atggcagaaa gtctggcgac agcaaccatt tacggtgata
    29761 atgtgccgct ctcctcgctg aaaagttatt ttggtcatac gcttggtgcc tgtggcgcgc
    29821 tcgaagcctg gatgagtctg caaatgatgc gcgaaggttg gtttgcgcct acgctaaacc
    29881 tgaataaacc agacccgaat tgtggcgcgc tggattacat catgcatgaa gcccgcaagg
    29941 ttgattgtga gtttttgcag agtaacaact ttgcctttgg cggcattaat acatccatca
    30001 ttattaagcg ctggccctga gttatacaac cacatggaca tcccatgtgg tgattcttca
    30061 aaaaatctga atcaatgaat atgaacctca aattctctta cgcatactga agagcattcc
    30121 gtataacgat tcagcagaca aaactttatc agtccacacg aatcaacgga tgcacagatg
    30181 acacgcccgg ttatcaccgg gcaatggttg ccataacggg taacataagg gactgaacag
    30241 ttgtctttag cgcaacgaac tgatctaacg tacctgaacg gacaacctga aaacagcctt
    30301 ccatcctgca ttgtccgcca gtgcttatta ccaaccacgt ctgttgcagt aaagtaatca
    30361 gttgcgaaca ttatttatca atcgtgacgc tggctcgcgc atggcagaaa aggtcctgac
    30421 tgagcaacaa caggcatcgt actgttgctg ctccatgttg tttatatgaa cgcatcccgt
    30481 ttgcaaggct tccgtgtttt tgacgttgac ggtagattgc tgctctatat ccttattgca
    30541 gccggtgctg cgggcctcaa tgagattcgc tgactctcgc cttattcacg caacgagctt
    30601 taagctcaga aaaacatcca agtttggtga gtcacagtct tacctgtcgt tgtcatcaat
    30661 ataggaatgg ataatctgga gagcgatact gcaggctgat attgcggttt tgcgacaagt
    30721 ttttagcatc cgcttctacg ccaaatacct gaagtaggaa tggatgcgtt agcagcatac
    30781 atccggaact gcgcggggaa tagccggaaa cggctatccc taccacaata gctaactttg
    30841 taatagcgct cttttaggct catgtactct gacgtaattc tatctggtag ttgaattgca
    30901 cgatacgcga ctcttctgat tcatcatgat tattattcaa tagtccaatc agtaacttac
    30961 ctgtctctat ccccattttt ttgtagtcaa tacggatgga tgtcaaagtt gggtgtgttt
    31021 gatcgcaatt gtctgaaccg tcaagacaag ctacagcgat atttccaggg atttttaaca
    31081 aacgccgctg gcactcaaac attataccca gcgcaatctc ttcgtggctg caaatcacag
    31141 catccagctc tggttgtcgc agtaatatct cgtttaatgc gtaacgcccc aattgcagac
    31201 ttgcggcatc cggcatggtg ataacaagat cagtgttctg gtaatggctc agcattgctt
    31261 tatgccagcc gttcagttgc tgacgttgca ggcggttgtc catatgcgca ccgataaatc
    31321 cgatattctt ataaccctgc tccagcagat gttcggtcag tttatgtgat gcttcaaaaa
    31381 atgctgtttc gatggtaatt tgagccggac tgaaagggga accgataacg ttaatggtag
    31441 gtatatttgt tttctcgagt atctggtgag ttttatcagc caactgagaa ccaaaaataa
    31501 ctaacgccgc cgggttgcct tgaagaagcg tcatcagaat ttcagcttct ttgagctgat
    31561 tatactcatg acacccaagc agtagctgga attcattttt attaagcacc tgctgtaatg
    31621 actgcataaa gcgggagctg gctttatctg tcagggaagg aaccagtacg gcgactgtat
    31681 gactgtgacc agatgcaagg gcgccagcag ttctgttggg aatataaccc aactcttcta
    31741 cggcctgttc tatcttttcc ctcagcttgt cagaaacctg ttccggtgtt ctcagcgcgc
    31801 gggaaacggt catggttccg acacctgcaa agttagcgac ctcctgcagc gtcacccgtc
    31861 cggtaccacg gcgcttacgt gttttttcca tttcgactcc atggataatt acaaccagct
    31921 gttttcatta cttttatttc accattactc tttccagttt ttgcccacgg tagcaaatat
    31981 cctgttgcat tgtatatgca ggaaccgaat atagccaaag catggcggca gaaaaatgat
    32041 agcgctatca caaagattgg tagcgttatc agatagcgct atcaaatgcg acaatcatca
    32101 cattaattaa tgcgtaaaat atctactctc ctgttaatta tcatcgggag ttatcagtag
    32161 catgttaaaa aatctattaa atacagaggt cgttcaggtc gtggaacagg tcaaagactg
    32221 gcgggaagcc gtggcgattt catgccgacc gttgattgaa aacggctcta tcgaaccgcg
    32281 ttatgttgat gcgatctatc actcgcacga cactatcggt ccgtactatg tggttggccc
    32341 tggtattgcg atgcctcatg cgcgcccgga agagggggct aacaagctgt cattagcact
    32401 gaccctgatc ccttcaggtg tgaattttga tgccgatgaa aacgatccag tgaaattact
    32461 gattgtgctg gctgcaaccg acagtaccag ccacattgaa gcgatttctc aactcgccaa
    32521 gttattcgat aacgaaaaag acatacaggc cattttgacg gccaaaacga cgcaggacat
    32581 tttgtctgtt attgcgcgct attaatccag aggaacattt atgaaaatta cagtggtttg
    32641 cggtaacggc ttaggcacca gcctgatgat ggaaatgagc attaaaagca ttttgaagga
    32701 tttgtccgtc agcgctgatg ttgatcacgt cgacctgggc tccgcgaaag ggacgccgag
    32761 tgatatctat gtcggcacca aagacattgc cgagcagtta gccgcgcagt ctgtcgcagg
    32821 caaaatcgtc gcactggaaa atatgattga taagaaagcc atgagagaac gtttgtccgt
    32881 ggtattaacc gaattaggcg cactgtaagc tgggggcgat atggatttct ttcgtttttt
    32941 gatgagcgat gtactttccg aacccgcagt gctggtcggt ttaatcgccc tgattggcct
    33001 gattgcacag aaaaaaccgg taaccgaatg tattaaaggt accgtcaaga ccattatggg
    33061 gtttgtgatt ttaggcgcag gggcgggttt agttgtgtcg tcactgggtg acttcgcaaa
    33121 cattttccag catgcctttg gtattcaggg tgtagtgccg aacaatgaag ccattgtttc
    33181 cgtagcgcag aaaagcttcg ggaaagaaat ggcgatgatt atgttctttg cgatggttat
    33241 caacattatg attgcccgtt tcacaccgtg gaaatttatc ttcctgaccg gtcatcacac
    33301 gttgtttatg tcgatgatgg tagcggtaat tctggcaaca gcaggcatga ccggcattac
    33361 gcttatagcc gttggctctt tggttgttgg cgtggcaatg gtctttttcc cggctattgc
    33421 gcatccgtac atgaagaaag tgaccggttc tgatgacgtt gccatcggcc acttctcaac
    33481 attatcttat gtattggccg gtttcatcgg cagcaagttt ggtaataaag agcactcgac
    33541 tgaagacatg aatgtgccga aaagtctgct gttcttgcgc gacacgccgg ttgctatctc
    33601 ttttaccatg agcataattt tcctggtgac gtgcctgttt gcgggtgcag atgcggtgaa
    33661 agagcttagc ggtggtaaaa actggttcat gttctctatc atgcaatcca ttaccttcgc
    33721 agctggcgtg tacatcattc tgcagggtgt gcgcatggtg attgcggaga ttgtcccggc
    33781 attcaaaggt atttcagaca agctggttcc gaatgccaga cctgctctcg attgcccggt
    33841 cgtcttccct tacgcaccta acgctgtact ggttggtttc ctgagtagct ttgctgcagg
    33901 tttaatcggt atgttcacgt tgtatctgct gaacatgatc gtgattattc ctggtgtggt
    33961 gccgcacttc ttcgtaggtg cagctgcagg ggtattcggt aatgcaacgg gtggacgtcg
    34021 tggtgcgatt ttgggtgcct tcgctcaggg cttgcttatt accttcctgc cagtattctt
    34081 attgccggtg ctgggtgata ttggttttgc taacaccacc ttcagtgatg ctgactttgg
    34141 cgcactgggt attttgttag ggattatcgt tcgctaatat tttgatgaca ttaatatcct
    34201 ccaggatttc aggaacgtgg aggatattaa ttcctcttat tttctccatt tttcttccag
    34261 tcaggactgc atccgattaa tcgtaagcgc tccgagaagt acgtagcgta aggattattt
    34321 tacagctgag aagtttcagt gcagcagttc atgcacacgg ttcgacggct agtcattgat
    34381 cttcataatt acctcgcgta aaaaacggga taaggccgct catccagcgt catctccaca
    34441 ggtaataatt caacgccaat gccagaagag ttcgttaccg gaagacgccg tgatatacgc
    34501 cctttgatca gcgagagcct gagccatttg aacagaagat tatcattgat atcatgctcc
    34561 cgggcaatac gggcaacaga accccttggt tgtaaagcca gtttaaccat ttgaagttta
    34621 aactcatttg aaaatgacct gcggggttca actgataatg ttttttgttc cataacaggt
    34681 gtccactagt tgaaaaggtg ggtacctatg ttaccaatac tggcttaatg gctacataca
    34741 gcagtcggtt tacgcataca cttttatagt gcactccggc ctcatgaata taacggtgga
    34801 ttaccgccga acgaatcgga aaatagatac tggaaaaaaa ctgaaattgt gaccagtttt
    34861 tgttgaccac tacaaataaa taaaagagtt gcagtgcttg ctcggtaaga aaacaatgga
    34921 ggttaagctc ctcaaagaag ctgtggagta caccactcta cagtagttgc gcaaatttgg
    34981 agtggacgat aatgtgatcg tctataaggg caacgctatc atagtcttgt cctggcgggt
    35041 aaaaaaacgc gcttacctta acgataagcg cgccgctgtt caggccttga gtggttattc
    35101 aattcctgtg gtgactgtaa aagtgcgcgt ttgctgcggt gcaacctgaa tcagcgtgcc
    35161 attacgttgc gcggcaagat acccctcagg ccgacaggtt gcaggtaatg caaaggcggc
    35221 tacctgttgc tctccgttat aaaggatcca gcgtgtcaca taatttagtt cagcactgta
    35281 gaaacgagta acaaacgtag tgccatcggg agagatcatg cgaaactctg gctgatctgt
    35341 ataagcgtcc agtttgtctg caaagaagac aatttctgga tcataaaatt ccggttgact
    35401 cagcgtcgac agagaggctt ctccctgcat aatccgttga ttaaacgcca gccactgagc
    35461 ggtgggatta acatgcgaag gcactgattc acgcaatctt aatatttcgt ccgggatatt
    35521 ctggctgaat gtagcatttg gtatatatgc ataattcatg tggcacatat attgtagtgg
    35581 catatctaca gaagccagat tggttacggc catcttaata tcgaacagtg tagaggattt
    35641 gtgaaggacc actgttggct gagccagata atgatgaccg aaacccatta catactcgta
    35701 acgcccgtta aggcgtaaca tatctccgtc taattccagc catgcttcat ccatcgcggc
    35761 acaggccatt tcaccgtgta gcagatgagt atcttccgca gatgggcagc cattagccag
    35821 caaacctgaa tgaaaagcaa aacagccata ggtctctatc acctctgtcg ccggtttagg
    35881 ctggcgaaac atattgcaca tggtgaggcc gtgtccatca aattgcgcat cccaaatcat
    35941 ctgccccatc cagggaagaa taatcaaatg tccacgactg tttgcaattt taagcccctc
    36001 gacaccgctg tcatatcgaa aagacgtgac agtaaaatca ctattttcca gcaagatacg
    36061 aggtttctcg ccaaaaagcg cccgccacaa attaatacgc gtactcataa cggttctcct
    36121 caggacgctg tgacttcagc cagtgcggta cgtactttgc tttcacgcca gaagtagact
    36181 ccgacataga caaagcagag catagaaacc aggaatgaaa gctgtagtga gtggaacata
    36241 tctgcaatat atccctgaat tgccggaacc accgcggcac cgacaatagc cataacaatg
    36301 actgctcctg ccatttctgt atgttcgtta tcaactgtat ccagtgttcc tgcatagatc
    36361 gtcgcccagc aagggccaaa caaaacactt accaggacgg cgacatagac cgcgctgaaa
    36421 cttggagcca gtgcaacata tgccaggaac agcgccccta taacggaata gagaatcaat
    36481 actttttccg gattaaaacg cgtcataagg atattggcta taaacttgcc aataaagaag
    36541 caggcaaagc tatagaccat gaagtttgaa gcatcacgtt cgttgatatc gcccaactcc
    36601 agcgccagac ggatggtaaa tgaccatact gcgacctgca tacccacata aaggaactgc
    36661 gccacaatac cgcgacgaaa gcgcggattt ctagccagat agcgcagcgt atccattgct
    36721 gacgggcgtt tatggtgact tgtctgtgcc actttacagg ttgggaagcg ggttaaaagg
    36781 aacaacacca tgaccacaac cagaatcata atcatatact tatacggttc aagggtgttc
    36841 tctaacatca gcaccttaaa gttgtgaatt tgctcggcgt tcattcctga catctgcttt
    36901 tcaaggcttt ccccctcgga gaaaaccaga tatttgccca ataaaatacc agacgcagca
    36961 ccaatcggat aaaaggtctg gctgatattg agccgcaatg tggcataggc ttttggaccg
    37021 atcattgaac tgtatgtgtt cgctgcagtt tcaaggaaac tcaggccaat cgcaatcgca
    37081 aaaatagctg caaggaacat agtgtaggtt gccatatgcg aggcagggaa aaaaagtgta
    37141 caaccaccaa tatacagcgt caggccaatt aaaattgcca ccttataact ggtcttttta
    37201 atcacaaggg atgctggtat tgcaattaaa aaataacctc cataaaatgc gctctgcacc
    37261 aatgctgaag caaagttact tagcgaaaat acacttttga attgagtgat taatatgtca
    37321 tttaatgcag ctgcgcatcc ccatagcggg aataaacacg ataacaaaat aaactggaac
    37381 aagggagtct tattcagata cccatcaggc atctgaatga tgtttttatc gttcatagtg
    37441 ctacctttaa ctgtgcagga tgattattcg tttaaggtta aaaattcatt aaattgttca
    37501 atactcggat aagatgattg cgtacctttc cctgtgacgc tgaaagcggc aaagagagcg
    37561 gcttttttca aagcggcttc aacatcaccg ctttgaacat aataatggga aaagcaacca
    37621 ataaatgcgt caccagcgcc actagtatca acagcattta ctttgaatgc aggaacatgg
    37681 acttcctgat cgcgggtcat ccataatgcg cctttttcgc tcatggtaac aataatattg
    37741 ttcagccctt tatcaactaa cgaacgtgcg gccaaacgaa tatgatcata agtatcaacc
    37801 gacataccgg ttaatatttc cagttctgtt tcattcggga taaagaaatc acatttgcag
    37861 gcataagaca tatctaactc acgcaatgcc ggagccggat ttaataacac ttcaatacca
    37921 tttttcttac caaactcaat cgcgtggtaa actgtttcca gttgaacttc cagttgtaaa
    37981 acgatcaatt tgcatttttt cagatcttct gcagctcgat cgatatcttc cggggaaaga
    38041 aatttattcg ctcccttaat tattaatata ctattgctcg agttggcatt aacaaagatc
    38101 ggtgcaacac cactgctggt acaggggact ttctcaacat aagtggtatt aattccccat
    38161 gattcgagat tacgaatagt attatccgca aaaatatcat cacctacttt agtcagcatc
    38221 aggacttttg aattcaattt agccgccgcc accgcttgat tagcaccttt cccaccacat
    38281 ccgattttga aggcaggtgc ttccagagtt tctccttctt taggcatctg attagtgtaa
    38341 gtaatgagat ccaccatatt ggaaccaata actgcaatgt ccatttcact acctcttata
    38401 aactttcgca taacaatggt atttaaataa cattagcatg ttacttttac atcatttgtg
    38461 actgagatcg cgattagcac atcaactcga tgtttattta atagacttcc agtcttatca
    38521 ctcaggccaa cactatctaa tcataagcaa cctaacagga ttaataccga aaattcagca
    38581 gtctataccc ttttcatt
//
LOCUS       DAPHJE010000036        38433 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPHJE010000036 DAPHJE010000000
VERSION     DAPHJE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.036"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..2386
                     /gene="pdeF"
                     /locus_tag="RH222_001828"
     CDS             143..2386
                     /gene="pdeF"
                     /locus_tag="RH222_001828"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV3796868.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2425..3966)
                     /gene="ppx"
                     /locus_tag="RH222_001829"
     CDS             complement(2425..3966)
                     /gene="ppx"
                     /locus_tag="RH222_001829"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV3796869.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3971..6037)
                     /gene="ppk1"
                     /locus_tag="RH222_001830"
     CDS             complement(3971..6037)
                     /gene="ppk1"
                     /locus_tag="RH222_001830"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV3796870.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6208..6846)
                     /gene="purN"
                     /locus_tag="RH222_001831"
     CDS             complement(6208..6846)
                     /gene="purN"
                     /locus_tag="RH222_001831"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV3796871.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6846..7883)
                     /gene="purM"
                     /locus_tag="RH222_001832"
     CDS             complement(6846..7883)
                     /gene="purM"
                     /locus_tag="RH222_001832"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV3796872.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8208..8834
                     /gene="upp"
                     /locus_tag="RH222_001833"
     CDS             8208..8834
                     /gene="upp"
                     /locus_tag="RH222_001833"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV3796873.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8920..10209
                     /gene="uraA"
                     /locus_tag="RH222_001834"
     CDS             8920..10209
                     /gene="uraA"
                     /locus_tag="RH222_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV3796874.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10259..11005
                     /gene="hda"
                     /locus_tag="RH222_001835"
     CDS             10259..11005
                     /gene="hda"
                     /locus_tag="RH222_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV3796875.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11143..11502)
                     /gene="arsC"
                     /locus_tag="RH222_001836"
     CDS             complement(11143..11502)
                     /gene="arsC"
                     /locus_tag="RH222_001836"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV3796876.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11523..12986)
                     /gene="bepA"
                     /locus_tag="RH222_001837"
     CDS             complement(11523..12986)
                     /gene="bepA"
                     /locus_tag="RH222_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV3796877.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13199..14260
                     /locus_tag="RH222_001838"
     CDS             13199..14260
                     /locus_tag="RH222_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV3796878.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14298..15146)
                     /locus_tag="RH222_001839"
     CDS             complement(14298..15146)
                     /locus_tag="RH222_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDV3796879.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHYIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15168..17180)
                     /gene="hyfR"
                     /locus_tag="RH222_001840"
     CDS             complement(15168..17180)
                     /gene="hyfR"
                     /locus_tag="RH222_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV3796880.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNVYYRYHDDGSDVTATAEYQGEMIDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17210..17623)
                     /gene="hyfJ"
                     /locus_tag="RH222_001841"
     CDS             complement(17210..17623)
                     /gene="hyfJ"
                     /locus_tag="RH222_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV3796881.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQAPAIYLMARKIA"
     gene            complement(17616..18373)
                     /gene="hyfI"
                     /locus_tag="RH222_001842"
                     /pseudo
     CDS             complement(17616..18373)
                     /gene="hyfI"
                     /locus_tag="RH222_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18370..18915)
                     /gene="hyfH"
                     /locus_tag="RH222_001843"
     CDS             complement(18370..18915)
                     /gene="hyfH"
                     /locus_tag="RH222_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV3796882.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18925..20592)
                     /gene="hyfG"
                     /locus_tag="RH222_001844"
     CDS             complement(18925..20592)
                     /gene="hyfG"
                     /locus_tag="RH222_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV3796883.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20582..22162)
                     /gene="hyfF"
                     /locus_tag="RH222_001845"
     CDS             complement(20582..22162)
                     /gene="hyfF"
                     /locus_tag="RH222_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDV3796884.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22167..22817)
                     /gene="hyfE"
                     /locus_tag="RH222_001846"
     CDS             complement(22167..22817)
                     /gene="hyfE"
                     /locus_tag="RH222_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV3796885.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22829..23936)
                     /locus_tag="RH222_001847"
                     /pseudo
     CDS             complement(22829..23936)
                     /locus_tag="RH222_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(23948..24058)
                     /locus_tag="RH222_001848"
     CDS             complement(23948..24058)
                     /locus_tag="RH222_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HDV3796886.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFYSPH"
     gene            complement(24075..25019)
                     /gene="hyfC"
                     /locus_tag="RH222_001849"
     CDS             complement(24075..25019)
                     /gene="hyfC"
                     /locus_tag="RH222_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV3796887.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLTPLFTGISRQIRARMHSRRGP
                     GIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGG
                     DLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
                     STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEY
                     SGAGLALAKWGLGLKQVVMAALFVALFLLFGRAQELSLACLLTSLVVTLLKVLLIFVL
                     ASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25030..27047)
                     /gene="hyfB"
                     /locus_tag="RH222_001850"
                     /pseudo
     CDS             complement(25030..27047)
                     /gene="hyfB"
                     /locus_tag="RH222_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27047..27664)
                     /gene="hyfA"
                     /locus_tag="RH222_001851"
     CDS             complement(27047..27664)
                     /gene="hyfA"
                     /locus_tag="RH222_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV3796888.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(27917..28387)
                     /gene="bcp"
                     /locus_tag="RH222_001852"
     CDS             complement(27917..28387)
                     /gene="bcp"
                     /locus_tag="RH222_001852"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV3796889.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28387..28959)
                     /locus_tag="RH222_001853"
     CDS             complement(28387..28959)
                     /locus_tag="RH222_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV3796890.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29105..29983
                     /gene="dapA"
                     /locus_tag="RH222_001854"
     CDS             29105..29983
                     /gene="dapA"
                     /locus_tag="RH222_001854"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV3796891.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30000..31034
                     /gene="bamC"
                     /locus_tag="RH222_001855"
     CDS             30000..31034
                     /gene="bamC"
                     /locus_tag="RH222_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV3796892.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31247..31960
                     /gene="purC"
                     /locus_tag="RH222_001856"
     CDS             31247..31960
                     /gene="purC"
                     /locus_tag="RH222_001856"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV3796893.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32114..32341
                     /gene="vapB"
                     /locus_tag="RH222_001857"
     CDS             32114..32341
                     /gene="vapB"
                     /locus_tag="RH222_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV3796894.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32341..32739
                     /gene="vapC"
                     /locus_tag="RH222_001858"
     CDS             32341..32739
                     /gene="vapC"
                     /locus_tag="RH222_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV3796895.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLAVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32886..33749
                     /locus_tag="RH222_001859"
     CDS             32886..33749
                     /locus_tag="RH222_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV3796896.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33764..35779
                     /gene="tmcA"
                     /locus_tag="RH222_001860"
     CDS             33764..35779
                     /gene="tmcA"
                     /locus_tag="RH222_001860"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV3796897.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35853..36551
                     /gene="ypfH"
                     /locus_tag="RH222_001861"
     CDS             35853..36551
                     /gene="ypfH"
                     /locus_tag="RH222_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3796898.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36661..36861)
                     /locus_tag="RH222_001862"
     CDS             complement(36661..36861)
                     /locus_tag="RH222_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV3796899.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36889..38016)
                     /gene="dapE"
                     /locus_tag="RH222_001863"
     CDS             complement(36889..38016)
                     /gene="dapE"
                     /locus_tag="RH222_001863"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV3796900.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38020..38376)
                     /locus_tag="RH222_001864"
     CDS             complement(38020..38376)
                     /locus_tag="RH222_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV3796901.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9344 a         9900 c        10111 g         9078 t
ORIGIN      
        1 tttgatatat atcaattgga actttcatat gacacttaga atcaatgctc tctatagtga
       61 tgaataatca tcattcgaag tcaggtggga tgcctgtctg aatacacctc cttcaggatg
      121 tgggggattc tgctgagcat ctatgaaact gaatgcaact tatataaaaa tacgtgataa
      181 atggtggggg cttccgctgt tcctgccttc tttaatcttg cccattttcg cccacattaa
      241 tactttcgcg catatttctt ccggtgaggt ttttctcttt tatctgcctc tggcactgat
      301 gatcagcatg atgatgtttt tcagctgggc ggcattgcca gggatcgcct tagggatttt
      361 tgtccgcaaa tatgcagagc tgggttttta cgaaacgcta tcattaacgg ctaattttat
      421 tatcattatc attctctgtt ggggcggtta cagggtcttt actccccggc gtaacaacgt
      481 ttcacatggt gatacccgtt taatttccca gcgtatattc tggcagattg tgtttcctgc
      541 aacgctgttt ctgatacttt tccagtttgc tgcatttgta ggattactgg cgagcagaga
      601 aaatctggtc ggtgtcatgc cttttaacct cgggacttta atcaattatc aggccttact
      661 ggtgggtaat ctgatcggtg tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc
      721 attttattta cgtagctatt attcgcaatt aaaacagcag gttgatgcca aagtcaccaa
      781 aaaagagttc gcgctctggc tactggcatt aggtgcttta ttgttgctgt tatgcatgcc
      841 gttaaatgaa aaaagcacaa tttttagcac caattatacc ttgtcattat tgctgcccct
      901 gatgatgtgg ggagcgatgc gctatggtta taagctgatt tcgctgctct gggcggtcgt
      961 gttgatgatc agcatccaca gctatcaaaa ttacattccc atttatcctg gctataccac
     1021 gcagctgacc ataacctcct ccagttatct ggtattctct tttattgtca attatatggc
     1081 tgtactggca actcgtcagc gagcggtagt cagacgcatt cagcggcttg cgtatgtgga
     1141 cccggtggtt catctgccaa atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg
     1201 gtctgcgctt tgttatttac gcatccctgg catggaaatg ctggttaaga actatggcat
     1261 catgctgcgg attcaataca agcaaaaact ttctcactgg ctgtcaccct tgctggaacc
     1321 gggtgaagat gtttatcagc tttcgggtaa cgatctcgcg ctgcggctga atacagaatc
     1381 gcaccaggag cgcattaccg cactggatag ccatctcaag caatttcgtt tcttttggga
     1441 tggcatgccg atgcaaccgc agattggcgt cagctactgc tatgtgcgct cgccagtgaa
     1501 tcatatctac ctgctgctgg gagagctaaa tacggtcgcc gaactttcca tcgtgaccaa
     1561 cgccccggaa aatatgcagc gtcgcggggc aatgtatttg caacgcgaat tgaaagataa
     1621 agtcgcgatg atgaatcgac tacagcaggc gctggaacac aaccattttt tcctgatggc
     1681 ccagccgatt accggtatgc gtggtgatgt ttaccatgaa attcttctgc gcatgaaagg
     1741 tgagaatgat gaactgatcg gccccgatag cttcttgccg gtcgcgcacg aatttggttt
     1801 atcgtcgagt atcgacatgt gggtcattga gcatacgctg caatttatgg ctgaaaacag
     1861 agcgaagatg cccgctcacc gttttgctat taatctgtct ccaacctcgg tatgtcaggc
     1921 tcgttttcct gttgaagtca gtcagttgct ggctaaatat cagattgaag cgtggcaact
     1981 tatttttgaa gtcaccgaaa gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt
     2041 gcagcatctt caggaattag gctgccagat tgcgattgat gatttcggca ccggctacgc
     2101 cagctatgca cggcttaaaa atgtgaatgc cgatctgctt aaaattgacg gcagttttat
     2161 ccgcaatatt gtgtcaaata gtctggatta tcagatagtg gcatcgattt gccacctggc
     2221 gcgaatgaag aaaatgcggg tagtggcaga gtacgttgaa aacgaagaga tccgcgaggc
     2281 ggtgctctct ttggggatcg attatatgca gggttatctt attggtaagc cgcagccgtt
     2341 aattgatacg ttgaatgaaa tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga
     2401 catttctcgt cggcccgcaa agcattaagc ggcgatttca ggtgtacttt cttcttcaat
     2461 tttcaaccgc cagccagcca cgccttccca gtattcttgc tccttttcca gatcaagcag
     2521 taccagcgca ttctgactaa accagtcatg cgggaaacgc agtgtccagt gactgtcatc
     2581 agtaatcagt gtcaatgttg gcggtgtggt ggttgcctga cgttgattgt ttagtaatac
     2641 gccaaggcgc aatagctgta tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg
     2701 cggcagatcg tcgagcttaa tcgctttacg gtgatagcgc accagtgtcg ccatcatcag
     2761 ctgctgttcc tgattaaaac ccggcaagtc actgttttgc agaatataag cggagtggcg
     2821 atgcaaaccg ctgtggttga tattcaaccc gacctcatgc agcatggcgg cccatcgcag
     2881 tagcgcctcc agttgcggat gcgccagctt cggttgctgt tcccgccact gttcgtacat
     2941 ttgcatagtg gtatccagca cccgtcgggc ctgctcgctg tcgatgtgat actggttggc
     3001 gaggctgctg gcggtgcgac tacgcacatc ctgatgacgg aaacgtcctt ccatttcata
     3061 cagtacgcct tcgcgtaaag ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc
     3121 aaacacaccg cataaaatcg ccagtccagg aacgaagact gttttccgct cttcggaaag
     3181 acccggtaaa ctcagcgatg cgaaattacg gtgacgtaaa acttctttta ccagtttttc
     3241 cagacgttcc ggggtaatta tcccgtcttt ctcgcccatt tccattaaca cttcatgggc
     3301 ggcttttatg gtaccggaag cgcccattgc cacgttccag ccctgaatac ggaattgcca
     3361 ggttaaagtt tccagttttt gtgctgccgc catacgagcg cgctgaaaat tctctttatt
     3421 gatgaccccg ccagggaagt aaagctgggc aaagctgaca caacccatcc ggcggctttc
     3481 aacgagaata ggttcgaaat tttcaccaat caccagttcc gtagatccgc cgccaatatc
     3541 aataaccagt ttgcgacctt tctccggttg ggtatgttcc acgcccataa aaatcagacg
     3601 ggcttcttca ttaccggaaa taatttcaat cgggtaggga atgacctttt ccgcgcgttt
     3661 cagaaagtca gtggcgttca gcgcctgacg cagcgtatgg gtgccgacta tacagacgct
     3721 ggcaggagaa aacccttgta gccgttcggc aaacagcgac agacagttca aaccgcgcgt
     3781 cattgcctct tcactcaaca tattatctgg ccccaggccg tccgccagat gcacccgctg
     3841 tttcaggcgg ccaataatct gcatggcacc atctaccaca cgggctatga ccatgtgaaa
     3901 actgtttgaa ccaagatcga ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat
     3961 tggcataggg ttattcaggt tgttcgagtg atttgatgta gtcgtaaatc gccaactgcg
     4021 cccgtacttt gcggcgattg ccgcggggaa cgtagcgatt actgagttct ttatcgatat
     4081 aacgtgcttt gaccgtatcg ctgaacaata tgtcgatgat gtccagcacc cgctgcttca
     4141 ggcgcggatc gagcagcggt gtcgccactt caatacgata atcaatattg cgcgtcatcc
     4201 agtcggcgga agaaaggtag acctttttat cgccgccatt ttcaaaaata taaacccggt
     4261 catgttcaag gtaacggtca acaatactga tggcacgaat gttgtcgcta atgccttcca
     4321 gattggggat cagcgaacac attccgcgaa ccagcagatt aaccggtacg ccggagctgg
     4381 aggccgcata cagacgatca accaggcctt tatcgacaag gttatttagc ttcagggtga
     4441 taccactggg cagcccttgc tgcgcgttgg cgatttcgcg gtctaccatt tcatataaca
     4501 gacggcgaga gttttgcggt gacaccatta aataatcaaa tgtcaccggg cggtatgggt
     4561 tttcaataaa gttaaatacc cgccgtactt cgttggtgat acgcgcatct gcggtcagca
     4621 acgaatagtc agtataaaga cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg
     4681 cgtaacggac cacttcaccg ttttctttac gtgaaatcag gaacagtttg gcgtgaattt
     4741 tcagccccgg cgcagagaag ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt
     4801 gaatgttggc ttcttcgtcg aaacgcgcct gtaactcaac caccacggtg actttcttac
     4861 cgttatgtgc ggcgtggatc atcgagtcga tgatgcgtga atctttcgcc acgcggtaaa
     4921 tgttaatttt aatcgccagt acgctcgggt cgaacgaagc ctgacgcagc agttccagca
     4981 catgctcaaa ggtgtgataa ggataataga gcaacacatc gcgttcgcga atggcatcaa
     5041 aaccattgcg gaactgggct ttatcaaacc aaatgtggcg taaacgcggc agtggtttgt
     5101 tcaccagatt ggctttgccg acattgggga aattaataaa gtctttaaaa ttatgataac
     5161 gaccgccggg gacgatggag tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa
     5221 ccagcgcatt gggcatatcg cgctgataaa caaaacgcac cggctcagca gttaaacgct
     5281 gcttgagact ggaagacatc aactccatca ggctggcttc catctcatgc actaaatcgt
     5341 attcggcatc gcgggtcatc ttcattgaat aggcattcag cgcgtcataa tcaaagaagc
     5401 ctttgaaaat atcatcaagg cagtaacgca gaatgttatc cagaagaatc atcggcttgc
     5461 gtcgacgcgg tgcttctggc ggtaaattca caaagcgcgg cactttatct gatgggatct
     5521 ccagcagcgc gtaacggatg gtatcgccac ggataatttc caccgccaga taggtgtaat
     5581 catctttcag gaactgcact aagtcagtgt caggattgat taaaatcggc gtaatgtgct
     5641 gacgcagata ctgcttaaaa taatgacgaa gccagttttg ttgattgacg gagagctggc
     5701 gttcattaat caggaagatc tggttacgcg ccatctccag cagcagctcg ttgtagaggc
     5761 cgtcgaattc ctgatcggct ttcagcaccc gggactgaat tttgcccagt aaatggcggg
     5821 aatgagagtt ggagccttgt tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc
     5881 ggactttata gaactcatca aggttattgg aatagatccc caggaaacgc atcctttcaa
     5941 tcagcgggtt agatttgtcc gccgcttcct gaagcacgcg ttcattgaac gataaccaac
     6001 tgagctcttt ttcgatgtat agcttttcct gacccattac cacttttact ccgttttatt
     6061 cacgggacac tgcctggata ttatggcgag caatattggc agatgtccaa ctatgccaga
     6121 aaagggggaa aaaaactgct ggagcttgcg atttacaggt gaattgaaaa acgccagcgg
     6181 cagagctggc gctttaacta cggggcatta ctcgtcggca gcgtagccct gcggcggcag
     6241 acgttgacca tccagccacg cggcgttttc gtgcattttc agacgaccat cggcaaacca
     6301 gctaatcacc agtggataaa tagcgtgttc ctgggtttgc acgcgggcgg tgatgtcatc
     6361 ttccgtatca ccagcaaata ccgggacttt cgcctgtaaa ataaccgggc caccgtccag
     6421 ttcatcggtg acgaaatgca ccgatgtacc gtgctcttca tcaccatttt ccagcgcctg
     6481 acgatgggtg tgtaatccgg gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg
     6541 cccggcatag tgggagacaa acgccgggct gagaatgcgc ataaaaccag ccagcacgac
     6601 cacatcgggt gcgtacatgt cgatttcatg aatcaactcc cggtcatagg cttcacgact
     6661 gtcaaacgcg ctggcgatga gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag
     6721 gccgaacgcg tcggccttat tgctgaaaac tgcccgtacg gtgcctttaa ttttgttagt
     6781 tttacaggcg tcaataattg cctgtaaatt acttccgttg ccggaaataa gcaccacaat
     6841 attcattatt cgataaccac gcgttgttcg gaatcagagg ctttgatgat accgattttc
     6901 cacgcgtttt caccgttggc gtttagcagg gcgagggctt tgtccacttc cggagcaggc
     6961 agggcgataa tcatcccgac gccgcagttg aaggtgcgat acatttcatg gcgctcaacg
     7021 ttacctgccg tttgcagcca gttgaacact tccggccact gccaggaaga ttcatcaatc
     7081 actgcctgag tattatctgg caatacgcgt ggaatgtttt cccagaagcc gccgccggtc
     7141 aggtgcgcaa tggcatgcac atcgaccttt tcaatcaact ccagcactga cttcacgtaa
     7201 atgcgggtcg gtgccagcag atgatcggct aatggcttac catcaagttc ggtggtttgc
     7261 ggatcacaac cgctgacttc aagaattttg cgcaccagcg aatagccgtt cgagtgcgga
     7321 ccgctggaac cgagtgcaat cagcacatcg ccgtcgctga ctttagagcc gtcgatgatt
     7381 tctgattttt ctaccacgcc aacgcagaaa cccgcgacat cgtaatcctc accgtgatac
     7441 atccccggca tttctgccgt ttcgccaccc accagtgaac agcccgattg cagacatcct
     7501 tccgcgatac cgctgatcac cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa
     7561 tagtcgagga aaaacagcgg ctctgcacct tgcaccacca ggtcattaac gcacatggcg
     7621 accagatcaa taccaatggt gtcgtgacgt tttaagtcca ttgccagacg cagcttggta
     7681 cctacgccgt cagtgccgga aaccagcacg ggttcacgat atttttgcgg caatgcacac
     7741 agcgcaccga agccgcccag accgcccatc acttccggac gacgcgtttt cttcactacg
     7801 cctttgattc ttccaaccag agcattaccc gcgtcaatat caacaccggc atctttgtag
     7861 ctaagagagg ttttatcggt cactgcttgg gtccccacgc gttacttgcg gtagaaaaat
     7921 aaaattcggc gcaattctaa cagggaaagc aaacgtttgc gagactgctt tacacaacct
     7981 ttttgcacgt cttttgccca ggcacgcggc gaaagaagac ttgtgccagg gtaaaggtta
     8041 gttttcggat ggaataatct tctttcataa ccatttgaat ataaaataac tttatctcaa
     8101 atcgttatca ttttgactaa agtcaacgaa aagaatattg ccgccttgaa gaaaggaggt
     8161 ataatccgtc gatttttttt gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg
     8221 aagtcaaaca cccactcgtc aaacacaagc tgggactgat gcgtgagcaa gatatcagca
     8281 ccaagcgctt tcgcgaactc gcttccgaag tgggtagcct gctgacttac gaagcgaccg
     8341 ccgacctcga aacggaaaaa gtaactatcg aaggctggaa cggcccggta gaaatcgacc
     8401 agatcaaagg taagaaaatt accgttgtgc caattctgcg tgcgggtctt ggtatgatgg
     8461 acggtgtgct ggaaaacgtt ccgagcgcgc gcatcagcgt tgtcggtatg taccgtaatg
     8521 aagaaacgct ggagccggta ccgtacttcc agaaactggt ttctaacatc gatgagcgta
     8581 tggcgctgat cgttgaccca atgctggcaa ccggtggttc cgttatcgcg accatcgacc
     8641 tgctgaaaaa agcgggctgc agcagcatca aagttctggt gctggtcgct gcgccagaag
     8701 gtatcgctgc gctggaaaaa gcgcacccgg acgtcgaact gtataccgca tcgattgatc
     8761 agggactgaa cgagcacgga tacattattc cgggcctcgg cgatgccggt gacaaaatct
     8821 ttggtacgaa ataaagaata aaaataatta aagccgactt taagagtcgg cttttttttg
     8881 agtaaagcgc ctataacaca taatacagag gataatacta tgacgcgccg tgctatcggg
     8941 gtgagtgaaa gaccgccact tttacagaca atcccgctta gtttgcaaca tttgttcgcc
     9001 atgtttggtg caaccgtcct ggtgcccgtc ttatttcata ttaacccggc gactgtactg
     9061 ttatttaacg gtattggaac gctgctgtat ctcttcatct gtaaagggaa aattccggct
     9121 tatcttggtt ccagctttgc ctttatttca ccggtattgt tactgttgcc gttagggtat
     9181 gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc
     9241 gtgaagaaag cggggaccgg ctggctggac gtgctgtttc cacctgcggc aatgggcgca
     9301 atcgttgccg tcatcggtct ggagctggcg ggcgtagctg ccggtatggc gggtttactc
     9361 ccggctgaag ggcaaacgcc agactccaaa accatcatca tctctattac caccctggcg
     9421 gtcacggttt taggttccgt gctgtttcgt ggtttcctgg caattatccc gattttaatt
     9481 ggcgtgctgg tggggtacgc gctctctttc gcaatgggaa ttgtcgatac cacgccgatt
     9541 attaatgctc actggtttgc gctgccaacc ctctatacgc cgcgcttcga gtggtttgcc
     9601 attctgacta ttctgccagc ggcgttagtg gttattgccg aacacgtagg gcacctggta
     9661 gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg
     9721 tttgctaatg gcttgtcgac cgtgatttcc ggcttctttg gctctacgcc aaataccact
     9781 tacggagaaa acattggcgt gatggcgatc acccgtgttt acagtacctg ggttatcggc
     9841 ggggcggcga ttttcgctat cctgctttcc tgcgtcggta aactggctgc cgctatccag
     9901 atgatcccat tgccggtgat gggcggcgtt tcgctgctgc tttatggtgt catcggtgct
     9961 tccggtattc gtgttttgat cgaatcgaaa gtggattaca acaaagcaca gaacctgatc
    10021 ctgacttccg tgattttgat catcggcgtc agtggcgcga aggtaaacat cggcgcggcg
    10081 gagttaaaag gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt gattttcaaa
    10141 ctgatcagtg tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc ggatataaca
    10201 gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat gggattctgt
    10261 ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca caccggcaca
    10321 gctctctttg ccactttatc ttcctgacga cgaaaccttt gcaagtttct ggccggggga
    10381 taactcctct ttactggctg cgctgcaaaa cgtgctgcgt caggaacata gcggttacat
    10441 ctatctctgg gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga
    10501 attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg gataaacgca cctggtttgt
    10561 tccggaagtg ctcgacggta tggagcattt gtcgctggtc tgtatcgaca acattgagtg
    10621 tattgcaggc gatgagttgt gggagatggc gattttcgat ctctacaatc gaattctgga
    10681 atcgggcaaa acacgattgt tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg
    10741 attaccggat ctcgcgtcgc gactcgactg ggggcagatc tacaaattgc agccactttc
    10801 tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc
    10861 ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca cgctatttat
    10921 gacgttggat cagttggatc gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt
    10981 tgtgaaagaa attctgaagt tgtagataag gtgtttattg tcggatgcgg cgcgaacgcc
    11041 ttatccgaac tacgcgaagt taatccatag tattcgtagg ccggataaga cgtggcaagc
    11101 gtcgcatccg gcaatgtgct tcaactagcg atggagcaca tcttaaccca cgatttccag
    11161 tacctgctcc ggtggacgac caatccgcgc tttgccattc gcgaccacaa tcgggcgctc
    11221 cattagcttc ggattatcca ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc
    11281 agccaggttc agttctttat aaagatcctc tttctggcgc atcaattcac gggcgctatt
    11341 catccccagc attttcagca aatcgcgcag cgttgccgca tcagcgggtg tctcaaggta
    11401 gagcaccact tctgactcca cgccgttttc tttcagcagg ttcagcgttt cccggctctt
    11461 tgaacagcgt gggttatggt agatttttac ctgtttggtc attgttcttc ctttaatgcg
    11521 aattacatct tggtataagg cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc
    11581 gcatcgtaac gcgcttgttg caggctgcct aatttcacct gcgaactggc gctactcaac
    11641 agcgaaatgg cctgatcgag tcgtccggcg agcgcataac cttctgctcg cgcagccagc
    11701 tcctgatcac ggttatttag cgcggcttcc gcctgtgcca gcaaatccca gccgttgctg
    11761 tcatctttat tattaaaggt gtagcgattc agaatattgg ccgcttcttg tggttgaccg
    11821 ccttgcagat aagcgttcgc caggttgagc tgcaacaccg gatttgtgcg taaatcgcgg
    11881 gcatttttca ggcgattgat cgcttcattg gctttgtttt gcccaagatc gatatcagta
    11941 gccagatcga gataccatgc gttgccaggt tctgccgcca gtaacggttg cagcgtttta
    12001 cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag cacgaccata ttgcgccgct
    12061 cgttgctgac gaacgtttcc tttcgcccat tcatccagca aatcactggt gagctggtta
    12121 cgtccggaat tatacatccc cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac
    12181 tgcaccacca tcggtcgcat ctgattagca cggttgcggg catctgccag acgactttcc
    12241 ggcaacgggt gagtcaataa aatttccggc gggcgcgagg agtaacgcgc ctgatcgagt
    12301 aatttttcga ggaaggttgg catcgcctgc ggatcgaatc ccgagcgttg cagcacctga
    12361 ataccaatgc ggtccgcttc ctgttcattt tgctgggtga aactgatcat cccctgacgc
    12421 gttcccgcca gtgtaccggt cagcgccgcc atccccgcct gcggactggc catcgccagt
    12481 aaaatagaac ctaacgcgcc gacccaggtc agcggcgcac tgcgttgttg atcttccatc
    12541 gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac tgaagccagt
    12601 tgactttcgt tatcggaata acggaacagg gcagagtgca gcaccacgtt gccgccaaag
    12661 aaagcaaagg cgttaatttc gtcgttattg atcagaaaaa aatgaaacgg tgtcttaacc
    12721 gaattggcat gcgaaaccag acgcatcccc agcgaattaa tatattgcgt taacagcggg
    12781 tcattaatta acggcgcgct gccgcgtagc tggcggacat aatagtcgcc catctgcatt
    12841 tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg
    12901 ctgtcggcaa acgccggggc tacctgacca atggtcatag cagcaatgag ggttgcaacc
    12961 aggttttttt tcaactgcct gaacataacc tctatcctgt atttctgaag atagtcattt
    13021 gaccgatact gttaaatatc gttcccaccg cgccgccttt gagtgtaacg gcgaagatta
    13081 gcccactcaa atgacttttc agtttcctga atccgtcata aaaagcgaag tcttttttgt
    13141 gacatttcga tacaattgcg gatcgcaatc ccgctcttga ggttcaggga aggtttttat
    13201 gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc gacccggaag cgattgcctt
    13261 gctggttatt ttagttgccg gatttggcat tatctttttc tttagtggcc tgcttgctcc
    13321 gttgctggtg gctattgtgc tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc
    13381 tattggctgc tcccgccgct gggcgacgtc gattgtattg gtggtttttg tcggtatatt
    13441 gctactgatg gcgttcgtgg tactgcctat cgcctggcaa cagggcatct acttaatccg
    13501 cgatatgccg gggatgctca ataagctttc tgactttgcc gccacgttgc cgcgccgcta
    13561 tccggcgtta atggacgcgg gcattattga tgcaatggcc gaaaatatgc gcagtcggat
    13621 gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac
    13681 cattgccgtc tatctggtgc tggtgccatt gatggtcttc ttcctgctga aagacaaaga
    13741 gcagatgctg aacgccgttc gtcgggtgct gccgcgcaac cgtggactgg caggacaggt
    13801 gtggaaggag atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat
    13861 cgtggtgggg atcgccacct ggctggggtt cttgctcttt gggctgaact attcgctgct
    13921 gctggcggtg ctggtcggct tctcggttct tattccgtac attggcgcat ttgtggtgac
    13981 cattccggtg gttggcgtgg cgctattcca gtttggcgca ggcacggaat tctggagctg
    14041 cttcgcggtg tatctgatta ttcaggcgct ggacggcaac ctgctagtac cggtgttgtt
    14101 ctccgaagcg gttaacctgc atccgctggt gattatttta tcggtggtga tcttcggtgg
    14161 tttgtgggga ttctggggcg ttttcttcgc cattccattg gctacgctga tcaaagccgt
    14221 gatccacgcc tggcccgatg ggcaaatcgc gcaagaataa tcttcagccc gacgaactgt
    14281 ttcgccgggc tgatttttca ggattcctga cgtaaataaa tagcccgata acaaatactc
    14341 accagcactg caccgccgat aatattaccc agcatcaccg ggagcagatt ggcggtaata
    14401 aaatggctga ccgtcagtgc cggaaaattg tctgcgctac tgtgcgccag ttgccagaag
    14461 gaggtagggg cgaaatggcg aatggcaatg gcgaacggaa tcacaaacaa attggcgata
    14521 tagtgctcaa agccactggc gacaaacagg gtgatgggca aaatcatggc gacgattttg
    14581 tcgcataacg aacgcccgca ataactcatc cacagcgcca ggcaaaccat cagattgcac
    14641 ataatgccga ggctgacaga ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt
    14701 aaaaccgcga ctccccacat cgcgttttca ctcatcacca gcccggaaaa ccagattaac
    14761 aaactgaaac aggcaatacc tgccagatta ccgcaggcca ccagaagtgc gttaatcagc
    14821 caagttcgcc aactaataac gccccgactt tttgccatta ccgtcattac cgacgaggtg
    14881 aacaggttgg tgccgcaaac cgccagcaaa ataaagccaa gtgtaaagca aaggccgccc
    14941 accagatggg ttaatgcctg tgaagacggg gcatcggcga taactgaaag gtaaaaagta
    15001 aatccgatcg ccataaacac gccagcggac atcgccatta aaaagaccga cactttactg
    15061 cgggcaattt tatgggcagc aatccgttca gcgattgccg cttttctggc gctcaactgc
    15121 aagtcgaaag agagtttgtt tctcatgcat actcctgcaa aagcagatta caacacctcg
    15181 cgaaccgaga ttcccagacg ctgcattcgt gacagcagcg tggtgcgctt catccctaat
    15241 cgtgtcgccg cgccacgggg cccggcaaca atgccattgg tttctcgcaa tacctgaaca
    15301 atgcgctgac gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt
    15361 gaactgcgaa gcgctgaatc ttcatttagc gtcggcagta aacggctttg tcggacatta
    15421 agatgtaaat tcagactgtt accgcgagtc agcagtaccg ctcgctcaat cacgttttcc
    15481 agctcgcgca cgttgcccgg ccagtcccac gacatcaact ggcgtagcgc ctcggtcggg
    15541 atggcgtcaa ttgaacgatt catatggcgc gccatttttt gcgtgaaatg ctttgctaaa
    15601 agagggatat cttccggacg gtcgcgcagc ggcggcaatt ccagtgggaa gacattcagg
    15661 cgataaaaga gatcgctgcg aaactggcga tcttcaacca tttgccacaa atcacggttg
    15721 gtggcggcaa tgactctgac atttaccggg atcgttctac tcccgccgag acgctcaatc
    15781 tcccgttcct gcaatacgcg cagcagttta ggctgaagtt ctaacggcag atcgccaatt
    15841 tcatcgagaa acaacgtgcc gccatcggca atttcaaaac gaccacgatg ggtattaatc
    15901 gcaccagtaa acgccccttt gtcatgaccg aataactcac tttccagaag actggcgggg
    15961 atggcagcgc agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc
    16021 gctctggcga tcacctcttt gccggttccg gtttcaccgc aaatcaacac tgtactgtcg
    16081 ctcttcgcca caatatctac ctgctggagt aggtcttcca ttgcctggct ttgatagata
    16141 atgtcaccga tgcccagatt cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc
    16201 aaactttcct gcaaatcggt catgctacgc caggcatcgg cattgccaac ggcaatagcg
    16261 atgcgatcgg ctatgtgttg tagtagctgg cagttttcct cactaaagag agtggaagag
    16321 gtatgcgcca gcaacaatgc acccggtgta tggttgccaa aggtaagcgg tataaggagc
    16381 gcagattcgc agccgttaga tgcaagtaag agtaataacg ggtcgcgttg ccagagaaac
    16441 agatcgtctg cacggtgcgt caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat
    16501 acactgtcgc caggcatatt gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg
    16561 caccacaggc tgaacttctc gttctttcga tgatcgccca gtaccatact gactgaagcc
    16621 agaccgaaaa aatgatggat ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga
    16681 tgtgacagca ctgcattagt gatatccacc agtacccgat gatgatcgcg atctttctcc
    16741 agcaaacgca gcgccacctg ctgttcttca tcattgagcc atgattgcag aaccggggtg
    16801 ataatgcgtg ccagccagtt aaacagtgtg caatcctggg gagtaaaacg gcagccgtgg
    16861 gtgcgaataa agcgtagctc cgccattcca ctactgccga gaaggacggc gtgacgcgaa
    16921 taatcgatca tctcgccctg atactctgct gttgccgtca catcgctgcc atcatcatgg
    16981 tagcggtaat agacgttgct tttgagaaca tgcagttcaa taagttgtac cgggctgaac
    17041 ggcggcggca gcgggatgaa ggcgcgtaat aaagacgcct taccgtgttt ctgcgctaac
    17101 cgctccgcga gcattccgtt taccgcttca attgttattc cttgcggcgg ggcaaacatc
    17161 gcctcgtctg acatagccat caacagtcct tattaatgag aaatccttct cacgcaattt
    17221 ttctggccat cagatagatg gccggtgcct gcacaatttc atccaacaga tgaatgaatt
    17281 gaatgctacg cgcctgccac acagacgttg cgtcatctgc cagtggcgcg aatgcccggg
    17341 tcaacagggc ggtgtggctg gcgtcaataa caatctcacc aaaagtcatc acccccagca
    17401 tcttacgtcg ggcttgctcc tcttcgacct gggcaagcca atcttcgtat tccgtcagtg
    17461 ggcagcggaa ggcgacattc agacaatcaa tcacgccaac gtgatggccg atagccagcg
    17521 agtaatacat tacctgagag ctgtgttccg gcatctcgtc gctactggcg acaaactttt
    17581 ttcgcagcgt ccagaaaaca atttccccgc actcttcagt catgtaaccc ccggagtacg
    17641 cgaaagagtt gctgaacgat actattgaga cgtggatcgt cggcatcgcg caaccaggtg
    17701 ttaacccgat ttcctgtagg atcgtcgctt aaatggcgca ggagccgatc gcaaatttct
    17761 cgcccctgac gatagcccgc cagccgccgc gcttctcgct caatggcgat acgctgtgat
    17821 ggcgggatct gcggccacaa cggttgcata gtcaccccgg tgggatcgcg ataatccacc
    17881 gcgtgaatct tctgttgcag caaaccgagc gccacggcga aaccgtgaat ggtggcggcc
    17941 ggtgttggcg ggcagccggg gatccaaaca tcaatgggga caatggtgtc gctgccgccc
    18001 cagacgctgt agagatcgtg gaaaatccgc cgccggcacc gcacgcgccg taggaaacac
    18061 agattttatg atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg
    18121 tgactgcgcc agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca accttaatgc
    18181 caaaacgttc tgcgtcgaat actggtgtaa tggcggcaaa aatttcgatt tcacaggcgt
    18241 tgcagccacc gcaatcgacg cgataaacgt aggctgagcg acggatatcc tgtagcaaat
    18301 gccgcttcat cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca tgttgtgtaa
    18361 gcactggact catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgagcag
    18421 cgtcgcgcgt tgtttgcact ccgggcagac gctcgcttgc gcccataaca tttcgcggtt
    18481 ttgtggcgca ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgctg
    18541 ggcaaacggg cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag
    18601 atcggcttta ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg
    18661 gcacacttct tcacaacgtc cgcagtaaat acaacgcccc agatagagct gccaggtgcg
    18721 cgaattttgc tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgca
    18781 ggcaccgcag gcaatacatt ggctgggcat caggtccggt ttcccgcgaa agcctgggct
    18841 gacctccagt ggcgcgaagg gatatttcac cgtcgcggtt ccggcgcgca taatagtttt
    18901 cagtaacttc agcatcttct gtccttactt cagcggcgaa cggttacgat caatgccgta
    18961 gcgttcgatc tctttatacg gcacggtttt tgactggcgc ttgcgcacat ctaccagcgt
    19021 cacacggtcg gtacaggagt agcagggatc aaggctaccg ataatcagcg gtgcgtcaga
    19081 aacggtattg ccgcgcagca tgtaacgcaa caccggccag ttggcgtagg tggcggcacg
    19141 gcagcgccag cggaacaatt tttggttatc accgagcatg ctccagtgca cgtcttcacc
    19201 gcgtggcgct tcaacaaagc ccagcgcgaa tgcgtgaggt ttatagctaa agccttcggt
    19261 cagcagtggg gtatccggca tgttgtcgag ggcaaattcc agcattgcca gcgaatcaaa
    19321 cgtctctttg acacggacca tcacgcggga gaaaacatcg ccgccggtaa aggtaaacag
    19381 tgtttttgga atattgccgt agtcggcgta ggggtgatca aagcgcaaat cacgggcgat
    19441 ttgtcggtcg agaatgccaa tgccctgagt gcgttgttcc atattcggcg tagcaagcag
    19501 catctctacc agctccgaca cgtcggcgcg catctcgcgc accagtttca gcgtttgcag
    19561 acgttgctct ttgagaatat cgcggcgaac accaccaatc agattcagac cgtaggtttt
    19621 acgcgacccg atcagcaatt ccgccatcgt catcgacttt tcccgcacgc ggaaaaattg
    19681 cataaagccg gtatcaaaac caacgaaatg gcaggagagg ccaaggttaa gcaaatgact
    19741 gtgtagccgt tcgacttcca gcagaatcga gcgaatagta tgtgctcgtt gcggcacctc
    19801 aatccccagt gcattttcaa ccgaattggt ataggccaca ctgtgggcaa aaccgcaaat
    19861 cccacacacg cggtccgata agaaggtcac ttcgttgtag cccatccgcg tttctgccag
    19921 tttctccatg ccgcgatgga cataaaacag gcggtaatca gcatcgacaa tttgctcgcc
    19981 atccacaaac aagcggaagt gacccggttc atcggaggtg atatgcagcg ggccgacagg
    20041 gatcacccgc gcatcgctgt tgccctcatt gataaacgca tacgtttcgg aatctgtcgt
    20101 cggttcaggg cgcagtcgat aatccatcgc atctttgcgc agcggatgca tatcttccgg
    20161 ccagtcatcg ggcaacacca ggcgacgctg atccggcagg ccaaccggaa tcagcccgta
    20221 catatcgcga atttctcgct cgccccagac cgcggcaggg acgcgcggtg tgactgacgg
    20281 aaactcccga ctgtcggcat cgaccagtgc cttcaccacg atccagcatt tttcggcccc
    20341 ttccattgaa agggcataat aaaccgcgta atgaccgtta agtgtccgct cgtcgttgcc
    20401 aaacaggact ggaagccagc catcatgttg ataataaaga taatgtacaa cgtcaggcag
    20461 cagattgatt ttcaccgtaa tggtgacctg ttcaggcgtt tgtcgctctt catccagcac
    20521 cgcgccgggg aactgcgttt tcagggcggc gagaatcgct tcgccacgat ttgacgatga
    20581 attaacgttc actgtgtttc tccgaaacag atgcggtgcc tgaaggcaaa aaatcatgcc
    20641 aggtgctacg ttgtgcaggc aggtcgtgcg tccctgagag gactatagtg gaagcgcccg
    20701 ccaggatcct gatgacaggt tgtggaatat gcgttcccat cgccagcatc atgaccagca
    20761 gaatcaccat tggcgaggtg gtcaaccagc cgagatcacc ccggttaacg gcctgcggcg
    20821 gtttcgccat taacacccgc gcagccatcc gtaccaggcc cgccagcacc agcgttaaca
    20881 gcaataacag caggacgata atcagcaggt gattacgtgc cagtccggcg gtaacggtca
    20941 taaattcgct aagaaaaatg ttgaagggcg gcatccctgc cagcgccagc gcaccgccgc
    21001 caaacagcac ggcggtaaat ggcatgattt tgagcatccc acagacgacg ttgagatcgc
    21061 gcgtgccgta cttgagcagt acattgccgg aaccgcagaa cagcagcgtt tttgccagac
    21121 tgtggtttaa ggtgtgcagc agcgcggcaa aaattcccag tggcccgcca atgcctagcg
    21181 ccaccgcgac cagtcccatg ttctccacgc tggagtacgc cagcagacgc ttaatgtccc
    21241 gctgtaccag aatgaaaaat gccgccacgg caaccgacaa catgccgaag atgagcaaca
    21301 accggttggg gaaatcgctg ccgatggcct ggcagataat gatgtaatag cgaattagca
    21361 ccaacagcgc gcagttcagc aatacggcgg agagcagggc gctgaccgga ctcggtgctt
    21421 cactgtgagc atccggcagc caggcgtgca tgggaaatag cccggttttg gtgccaaagc
    21481 caattagcac aaacacaaag gccaacagca ttaatgttgg gtcaagcaag gacgattgct
    21541 taagaacctc gctccagaat atcgccattt ctgcctgcgg cataacgctg gcgacgttgg
    21601 cgtataccag cacggtaccg aacagaccaa aagcgacacc aacagtacaa ataatgatgt
    21661 acttccatgc tgcttccagc gatgaacgct gaccgtaaat gcctaccaga aacgccgagc
    21721 ttaaggtggt ggcttcgatc gccgcccaca tcacaatcag gttattgctg gtaacaacca
    21781 gcagcatggt aaacaaaaac agatggaaga aaccgtagta atcgcacagc gtaacggggg
    21841 aaagctcgcc gtgtgccact tcatgacgca tgtagccaat cgagtaaaca ccggtgagaa
    21901 agccaatcac accaagaatg gcgaggaaca aaccgcccag accatcaata tgaagccaca
    21961 gtcccgcagc gaatatttct cctgcatcag cggcagtttg gaccacccag agtgccagaa
    22021 tcagcagcag tgtgatccct aagctatgta atacagtcac cgtacaagtc gcagagagcc
    22081 cccgtttccg gcaggcaaaa cagagcagcg aaaaaagcag cggcgtgagc aggagtaaag
    22141 cgaacatcac agaataactc atctcattat cccttcagcg cggtcaagtt gttcacgtcc
    22201 agcgtgccgt gggtacgcca tatttttctt gccagtaaca ccatcacaat gacggcgaag
    22261 atggcgtcgg tagcgatacc tatttccacc agttccggtg ctcgccaggc aagaagcgcc
    22321 agcaccagat gggagccgtt ttccatcagg cagtaaccaa aaatttgccg caggatattg
    22381 cgctggctga caatgcacag caggccaagc agaaaatgac ctaacgctac cgccagcgcc
    22441 ggtttcagcc cggtagccat cggtagcttc acgggctgaa cgacaaatgc gcaaagcagg
    22501 acaattaacg ccgcgagcag tgccatcatt gccggaccga ataacgcttt ttccgggatg
    22561 ttctggggaa tatttcgtgc agcgtaagtc atgattaacg gtaccagcag cactttggtg
    22621 ataaaggcac tggcggacca gatcagcagt tgctctgcgg cgaacaggca cgagagagtg
    22681 gcgaaaatag acaccagcac cagcgactgg caggcgtaaa atccgcatga caggcgatag
    22741 cttttgacgc taatcacaaa cagcgatgtc agcatcatca gtcccgccag attatttacg
    22801 atcatagaac cggtcattca tcatctcctt actgcaacca ggtcgcggcg attacgctgg
    22861 aaatcagcga catcacaatc agtacaatca acaccaggcg cattgaccct ggcagcggtg
    22921 cggcatcggc gacggcctcg ctcggtttgc caggcacaac gcgaccaaac cagcgaataa
    22981 accaggcgaa actggcgacc gattcaatca tcagcagaat catggcgggc agcaggatcc
    23041 agtactccac tgacaacgca aaacaggcgg caaacagcgg gaatttactg aagaagccgt
    23101 tgaacggcgg cacaccggta atcgccagcg ctgccacgca gaaacccacg cctggcaacg
    23161 gcagggtgtg caatacgcca cgcagacgcg gcaacaagcg cgtgccgcag ctgtaactca
    23221 gcgcacctgc tacaaggaaa aacaggcttt tagcgaacgc gtggttgacg atgtaggcga
    23281 tgctgccctc cagtgccagc cgcccacttt caccatcgat gcggcgtgga gataggcgct
    23341 gatcggtgtt ggcgcttcca ttgcgtccgg tagccacgct tgcatcggta gctgggccga
    23401 tttcccccac gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt cgccatgtaa
    23461 ctcgctcatc gcgctaagca caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag
    23521 atacaacccc aacgaaccga tatgggtgat aagcaacgct tttagtgctg aacgctgcgc
    23581 tttatcgctc tggtaataac tgatcaacgc ccaggagcag ccgccctaat ttcaaaaaac
    23641 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
    23701 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
    23761 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
    23821 agcgtactca cgcgatcaat gaccaggcca aacaatgcca catcccccac gttgaccaac
    23881 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
    23941 agcgtggtca gtgcggcgaa taaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
    24001 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
    24061 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
    24121 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
    24181 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    24241 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaaga gcagaaacag ggccacaaac
    24301 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    24361 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    24421 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    24481 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    24541 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    24601 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagcccggaa
    24661 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    24721 cccgcaaaag gggaaacggt aataaaaagt ggtagggcca tcgccagcac cagcatgctg
    24781 ctgattaata cccacggcat caggcggaac atcagacctg aagatatagg cgcaacttcc
    24841 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    24901 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagcggggt tagcagcaga
    24961 atcacggcct gtgctaacgc aaaaatgacc agatatccgt cgcaaagagt ttgtctcatg
    25021 gtgatttcct tagacggcaa tagcgattag cagcacaacc agtgcggcga ccacatacag
    25081 gcaatagaga cgaaagtcgc cgctttgcag atgctgtatt tctttggcca gccgttgggt
    25141 ggcgctcacg atggggcgga tcacccgctc atcccagaag ggttctgtgc tctgagccct
    25201 ggcggtgacg tgcgcaagac ctttatttag cctcagcgta gggtcgaact gttgttgtag
    25261 acgaaatagc gcagaaaaga ccacacgcag cggctgcatc acgccattgc ctgacggggc
    25321 catcgcattt tcccagccgt agccgcatgc ccaggcatct cctgtgcgac gaaacgctgc
    25381 gcggcgcgaa cgacaccaca gccagtaaag gccaggcagc aaaggcatcg ccagtagtaa
    25441 cagcaacagt aatgaggggg tgacccgtgt atgaaacgtg ccgggtacaa gtgctattcc
    25501 gctggcgaca gtgacgggag gggtattggt aaacgcatgg gcaatatgca ttatcttcgg
    25561 tgccagccaa ctggcactaa gcgcaattaa tacgcagagt gccgcgagca gtagcatggc
    25621 gacgatcatc gtatttggca cttcctgtgc ctcttcagcg tgtgtactgc gcggcgcacc
    25681 acagaaagta ataccgtaca ttttgacgaa gcacattact gccagcccac cagtgactgc
    25741 cagcattacc atagcaatag gacccgcaag ttgtagcgct acggcttcca cacgacttag
    25801 tgagaacagc gactgccagg tgtaccattc gctgataaaa ccattcagcg gaggaatggc
    25861 tgatatcgcg aggcaaccaa tcaggcatgc tgcggctgtc cacggcatcc gtttcgccag
    25921 tgcccccatt ttttccatgt cgtgggtatg caaacgcgaa ataatcgcac ccgcgccgag
    25981 aaatagcagc cctttgaaca gcgcatggtt gagcagatga aacagtgcgc cgagcagtcc
    26041 aaccacggtg agcagcgggt cgtgcagtga cagaccgtcc atcgccacac cgaccgccag
    26101 caaaataatg ccgacgtttt cgacggtact ccaggccagc agccgtttga tatcctgttc
    26161 cgccagtgca tacagcacgc ccaggagcgc ggagattgcg ccgatcgcca tcaccagagt
    26221 gccccaccac agaggcaaac ccgtttgcgc cagcagatcc atcgctactt tcaggatgcc
    26281 gaaaataccg attttgacca ttacgccaga catcaacgcc gaagcgtgcg atggtgcggc
    26341 agggtgagcg cgcggcaacc agctgtgcaa cggcatcatc ccggctttcg cgccaaaacc
    26401 gaaaaaggcc agcagaaaca ccgccgacgc cagccccgga gaaagtgaaa gcgtgcgaaa
    26461 ctggcaaaat cgaggctgcc gctttcgcgc cccatcagca agaaggcgat cataatcagc
    26521 accgatccgg cgtgggcgat aaaaaagtag agcatgccag cgttgatcga cgttttatcc
    26581 tgcctggcaa tgaccagaaa ccaggaagac agcgacatca tttcaaacag cacgatgaac
    26641 caaaaagcgt tgtccatcac cagcagggca accatcgatg cgatgaaaag attcataaag
    26701 aagcccatcg ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa tgaatagagc
    26761 gaacaaacca ccaccagcaa ggagataacc agcaccataa atgccgagag gctatccatt
    26821 cgcagggtta aatcggcaaa gtcaaaaggg gtggcaaaat gggcgactaa agttacacca
    26881 gcatgtaatt gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg
    26941 ccggaaagct taatagccag tctgtccaga ccgagtaaaa acagcgaagc cagactggca
    27001 aacagataga ggatcagcga ccaggttaat aattgcaggg catccattag tgttgctcct
    27061 gagtgaggga aaggggatcc tccccgccat tggcaaacca gcttgcggca aggcgctgtt
    27121 tttctttcat ctgacgttgc aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc
    27181 aagcgcgaac gcaggccgga ccttctggca agaaatcaca caggtcgcat ttcaccgcga
    27241 cggtctggac acccggttcc cagcgcagca acgcatgttg gggcagcacg ttttcttcgc
    27301 cgtctttgag tgagccttca gcctgaaaaa catattgcgc atgggcattc accggacggc
    27361 ttcctgaagc gctgattgcg ccaaatgggc agaccacggc gcaaagcttg cagccaatac
    27421 agaggctttc gttgagttgg atcgcatcat ccctctgaga gatggcattg accgggcaga
    27481 cctgcaggca aggggcttcc tcacagtgat gacacacgac aggggcagtg attgttgacg
    27541 tcttcgccag ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc acgtccgaac
    27601 aggcagcgag acaggtatta catcctgtac accacagtgg ttcggccacc acaaagcggt
    27661 tcatgtcggc tccttgaaaa tgatatgaaa cattgcattt catattgcat aaaggatgcc
    27721 attctgatta actcaatata ttcaataagt tatgtattga ctggattctg aacggtacaa
    27781 aaatgatggt cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg caggggaaag
    27841 cgagaatgtg atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg
    27901 gaatggagca aagtaatcag gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat
    27961 tgctggtttt gaaatcgtca aagacatgtt cgattttgcc atcagcgtca atcaggaagc
    28021 tgatgcgatg aatgccatcg taggttttgc ccatgaagga cttttcaccc cagacgccga
    28081 attgttcgca cacctggtgg tcctcatcag acaggagcgt aaagttaagc agctcttttt
    28141 ccgcaaaacg ggagagtttt tcgggtttat cggtgctgat acccagcaca tcaacgcccg
    28201 cttttttcaa ctcatccatg ttatcgcgta agccgcaggc ctgtacggta cagccggggg
    28261 tcatggcttt cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa
    28321 cttgttctcc gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg gctttcagtg
    28381 gattcattac ttaactccat cctgttcatc atgttgggaa taattgacga cgttaatact
    28441 gccttgtgca ttgagttctg tacatagggc tttgaacgct tgctcaatat ttgctgcgtc
    28501 cgcagatgcg gggctgtggg cggttatctg aatatgcaac tgcgcagccc gttcattttc
    28561 agcaggttgc gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc
    28621 tgtgaagcgt tcaattaaat gcggggagtc tgccacatcg acctgaaccc agacagatgc
    28681 tggcattggc ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc
    28741 ggcacctttc aacggtaacg ttgattcaat cagagtaatg gcattccatg aaccggaaag
    28801 cagcataata aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca
    28861 gccgcaacta ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa
    28921 cgcagtgatc accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg
    28981 tgagttgttc ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt
    29041 ctgcttgctt ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg
    29101 gcccatgttc acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt
    29161 ctgtcgggct agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat
    29221 cgtttctgtt ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt
    29281 ggtgatgatg acgctggagc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc
    29341 taacgctact gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg
    29401 ctgcctgacg gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt
    29461 caaagccatc gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac
    29521 tggctgcgat ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg
    29581 aatcaaagag gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga
    29641 tgattttgtt ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg
    29701 tcatggggtt atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa
    29761 actggcagca gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt
    29821 acacaacaaa ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact
    29881 gggtcttgtg gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg
    29941 tgagacggtc agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga
    30001 tggcttactc tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat
    30061 tactcgctgc ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct
    30121 acctggaagc ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga
    30181 cctccggtga ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg
    30241 acattcgtcc accagcccag ccgctggcac tggtttctgg cgcgcgtacc cagttcacgg
    30301 gcgataccgc ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg
    30361 ttagcgtgct gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac
    30421 tgaccaccga ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt
    30481 atcaaatctc tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgctgaacc
    30541 tggaacaggc gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga
    30601 tgatgaacgt tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc
    30661 aaaatcgtgc ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccga
    30721 tgctggtcgt acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa
    30781 aagtgggcat gaaagtgacc gacagcaccc gttcgcaggg taacatggcc gtaacttata
    30841 agccgctgtc tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg
    30901 gtgactataa actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc
    30961 cgaaaggtca tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg
    31021 cgtttagcaa gtaaaaatac agggctggaa tcatccggcc cttttttctg atatgatacg
    31081 caaacgtgtg cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg
    31141 tcggacgtag ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga
    31201 cagcaaaaga ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc
    31261 tgagttgtat cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt
    31321 gctcgaattc cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg
    31381 caaaggtatg gtgaacaaca agttcaatta cttcattatg agcaaactgg ctgaagcggg
    31441 tatcccgact caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga
    31501 tatggtgccg gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct
    31561 tggaatcgaa gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga
    31621 cgccatgcac gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa
    31681 agagaacctg gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact
    31741 gttcgatgat gctggtctga ttctggtcga cttcaagctg gagtttggcc tgtacaaagg
    31801 tgaagtggta ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac
    31861 actggagaaa atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaagccta
    31921 tgaagccgtc gcccgccgcc tgggtgtaca gctggactga tttttctggt tgcttgtttc
    31981 tttagccgga gggtactccg gctaaatgtc tttgataccc ccgctacaat acttctcatt
    32041 atctcccttt gacaggaaag tcataccagg ccaatatgta tatacataac gtatatccca
    32101 aaaagggggg gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt
    32161 accgaaagcg gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg
    32221 tacaagaatc atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt
    32281 gagtgcggat tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg
    32341 atgctgaaat tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta
    32401 catgtcagag agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg
    32461 atggagctta tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt
    32521 gaaggtttcg tatctcgttt ggcagtgctt gattatgata gtgctgctgc gacgcatacc
    32581 gggcaaatca gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg
    32641 atagcgggac atgctcgcag tcgcggtttg attgtggtta ccaacaatac ccgagagttt
    32701 gagcgagtgg cgggaatcag actagaagac tggagctaac attctctttt acttctaaca
    32761 ttcgtaaatt atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc
    32821 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat attgttgagg
    32881 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
    32941 ccggtggtcc gtctatgggc ggtcctggtt ttcgcctgcc aagcggtaaa ggcgggctga
    33001 ttttactgtt agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga
    33061 ctggacagcc ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag
    33121 ctgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
    33181 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
    33241 ccggctgcgg ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag
    33301 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
    33361 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
    33421 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat
    33481 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat aacatgcagc
    33541 agcaaggcgt tctggaaacc ggcgatctgg aagaggcgct gaacgcggcg caggccatcg
    33601 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagatagt ttcacacatg
    33661 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
    33721 aatgcaatac ttttggtaaa agcatttaac ttccggggca gggatggctg aactgactgc
    33781 gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
    33841 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga
    33901 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca
    33961 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
    34021 cgctgccttt gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
    34081 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg
    34141 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
    34201 taacgacgct atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
    34261 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
    34321 gttactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaaatc
    34381 ggcgttggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
    34441 aaaagcggca acggatgtat tggcacaatt tgcgggcgag aagtttcgct ttattgcacc
    34501 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc
    34561 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac
    34621 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
    34681 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
    34741 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
    34801 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
    34861 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
    34921 ccggatgatg gatgcaccag ggcaatattt tttacaggcg gctggcgaaa acgagattgc
    34981 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
    35041 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
    35101 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
    35161 gcgtcagcgg gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga
    35221 cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg
    35281 ctgcggtttt gtgctggtgc ggatgggtaa tcatcgtgaa gccagcagcg gttgctatac
    35341 ggcgatggcg ctgttaccga tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta
    35401 ccgtttacgt cgcgatgcgc aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga
    35461 tccactaaac gatgccgtcc tttctgacga cgactggctt gaactggccg gttttgcttt
    35521 cgctcatcgt ccgctattaa catcgttagg ttgcttattg cgtctgctac aaaccagtga
    35581 actggcatta ccggcgctgc gtgggcgttt acagaaaaac gtcagcgacg cgcagttatg
    35641 taccacactt aaactttcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc
    35701 gcaggcgctg ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
    35761 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg
    35821 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
    35881 gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg gataacccgg
    35941 tggcaatggg ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga
    36001 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcggtgcagg
    36061 gtattacgga agataatcgc caggcgcgtg tggatgcgat catgccgacg tttattgaaa
    36121 cggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacggcg ctcatcggtt
    36181 tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc cttgcttcac
    36241 gcgtcattgc ttttaacgga cggtatgcga gcctgccgga gactgcgtct acagccacga
    36301 caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat gcggttgcgg
    36361 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
    36421 gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc
    36481 cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg
    36541 aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg catcaggcat
    36601 cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg agcactcagc
    36661 ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa
    36721 atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt
    36781 gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca
    36841 atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag
    36901 ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag cgttcacaca
    36961 ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat
    37021 gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc
    37081 attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa
    37141 tggctgcccg gaaagccacc agtccaccgt ataacgcagt tgatgttttt caagcagggc
    37201 aagcacctgt gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg
    37261 cacaaacagt tcacccggaa taacattgtt actgcccgtt cccgcctgaa tattagcaat
    37321 ctgcatactg gtcgccggga aaaattcatt gccctgatcc cactcaatag ccactaattc
    37381 attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgcgggt aggccacatg
    37441 cccctgaacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac catttttcac
    37501 cacatcacca accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc
    37561 attacgtgcc atcaacgctt cgacgacttt taccgtaccg ttgtgggcac tggcttcttc
    37621 atcagaggtg atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg
    37681 ttctgccgct accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgtccgaa
    37741 taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat cggcgtcgcc
    37801 aggcggcacc acgtcggtat gcccggcaaa ggccaacgtt tcaccttgcc cacgccatgc
    37861 ccaaaaattc tgcgtatcgg caaaatccat gcgttcaacg gtaaaaccga tcgcctgcaa
    37921 acgttcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat
    37981 aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg
    38041 ctgataactg gaatcactga aacccagcag cataggctta ccaggcgcgc agagcaatgg
    38101 acgtttgata attgcaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat
    38161 tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tcagtaacgc
    38221 ttcccagcct aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg
    38281 gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat
    38341 ggtgtcacaa tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatctatga
    38401 gaataaatac tcatttaagg gtgaaaacat ttt
//
LOCUS       DAPHJE010000037        37976 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPHJE010000037 DAPHJE010000000
VERSION     DAPHJE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.037"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            179..340
                     /locus_tag="RH222_001865"
     CDS             179..340
                     /locus_tag="RH222_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796902.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(424..1659)
                     /locus_tag="RH222_001866"
     CDS             complement(424..1659)
                     /locus_tag="RH222_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV3796903.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDKFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(1806..1882)
                     /locus_tag="RH222_001867"
     tRNA            complement(1806..1882)
                     /locus_tag="RH222_001867"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Pro,seq:tgg)
     gene            complement(1925..2011)
                     /locus_tag="RH222_001868"
     tRNA            complement(1925..2011)
                     /locus_tag="RH222_001868"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1975..1977),aa:Leu,seq:cag)
     gene            complement(2032..2107)
                     /locus_tag="RH222_001869"
     tRNA            complement(2032..2107)
                     /locus_tag="RH222_001869"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2072..2074),aa:His,seq:gtg)
     gene            complement(2166..2242)
                     /locus_tag="RH222_001870"
     tRNA            complement(2166..2242)
                     /locus_tag="RH222_001870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2206..2208),aa:Arg,seq:ccg)
     gene            complement(2345..3730)
                     /locus_tag="RH222_001871"
     CDS             complement(2345..3730)
                     /locus_tag="RH222_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3796904.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     ITSHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3921..4661)
                     /gene="rffM"
                     /locus_tag="RH222_001872"
     CDS             complement(3921..4661)
                     /gene="rffM"
                     /locus_tag="RH222_001872"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV3796905.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4664..6016)
                     /gene="wzyE"
                     /locus_tag="RH222_001873"
     CDS             complement(4664..6016)
                     /gene="wzyE"
                     /locus_tag="RH222_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV3796906.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6013..7092)
                     /gene="rffT"
                     /locus_tag="RH222_001874"
                     /gene_synonym="wecF"
     CDS             complement(6013..7092)
                     /gene="rffT"
                     /locus_tag="RH222_001874"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV3796907.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAIIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGYVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVLEAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7089..8339)
                     /gene="wzxE"
                     /locus_tag="RH222_001875"
     CDS             complement(7089..8339)
                     /gene="wzxE"
                     /locus_tag="RH222_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV3796908.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8341..9471)
                     /gene="rffA"
                     /locus_tag="RH222_001876"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8341..9471)
                     /gene="rffA"
                     /locus_tag="RH222_001876"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV3796909.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9476..10150)
                     /gene="rffC"
                     /locus_tag="RH222_001877"
                     /gene_synonym="wecD"
     CDS             complement(9476..10150)
                     /gene="rffC"
                     /locus_tag="RH222_001877"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV3796910.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10128..11009)
                     /gene="rfbA"
                     /locus_tag="RH222_001878"
     CDS             complement(10128..11009)
                     /gene="rfbA"
                     /locus_tag="RH222_001878"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV3796911.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11028..12095)
                     /gene="rffG"
                     /locus_tag="RH222_001879"
     CDS             complement(11028..12095)
                     /gene="rffG"
                     /locus_tag="RH222_001879"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV3796912.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12095..13357)
                     /gene="wecC"
                     /locus_tag="RH222_001880"
     CDS             complement(12095..13357)
                     /gene="wecC"
                     /locus_tag="RH222_001880"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV3796913.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13354..14484)
                     /gene="wecB"
                     /locus_tag="RH222_001881"
     CDS             complement(13354..14484)
                     /gene="wecB"
                     /locus_tag="RH222_001881"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV3796914.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14540..15586)
                     /gene="wzzE"
                     /locus_tag="RH222_001882"
     CDS             complement(14540..15586)
                     /gene="wzzE"
                     /locus_tag="RH222_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV3796915.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15598..16704)
                     /gene="wecA"
                     /locus_tag="RH222_001883"
     CDS             complement(15598..16704)
                     /gene="wecA"
                     /locus_tag="RH222_001883"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV3796916.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLLFFARKVAKKVGLVDKPNFRKRH
                     QGLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRA
                     TIQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGID
                     GLLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGD
                     AGSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSP
                     DRQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFL
                     YGYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16944..18203)
                     /gene="rho"
                     /locus_tag="RH222_001884"
     CDS             complement(16944..18203)
                     /gene="rho"
                     /locus_tag="RH222_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV3796917.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18288..18389)
                     /gene="rhoL"
                     /locus_tag="RH222_001885"
     CDS             complement(18288..18389)
                     /gene="rhoL"
                     /locus_tag="RH222_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV3796918.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18530..18859)
                     /gene="trxA"
                     /locus_tag="RH222_001886"
     CDS             complement(18530..18859)
                     /gene="trxA"
                     /locus_tag="RH222_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV3796919.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18990..20255
                     /gene="rhlB"
                     /locus_tag="RH222_001887"
     CDS             18990..20255
                     /gene="rhlB"
                     /locus_tag="RH222_001887"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV3796920.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20389..21873
                     /gene="gppA"
                     /locus_tag="RH222_001888"
     CDS             20389..21873
                     /gene="gppA"
                     /locus_tag="RH222_001888"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV3796921.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWYLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21920..23941)
                     /gene="rep"
                     /locus_tag="RH222_001889"
     CDS             complement(21920..23941)
                     /gene="rep"
                     /locus_tag="RH222_001889"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV3796922.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24158..24607
                     /locus_tag="RH222_001890"
     CDS             24158..24607
                     /locus_tag="RH222_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV3796923.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVIPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24806..25087
                     /gene="ppiC"
                     /locus_tag="RH222_001891"
     CDS             24806..25087
                     /gene="ppiC"
                     /locus_tag="RH222_001891"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV3796924.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25174..26649)
                     /gene="ilvC"
                     /locus_tag="RH222_001892"
     CDS             complement(25174..26649)
                     /gene="ilvC"
                     /locus_tag="RH222_001892"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV3796925.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNTQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26799..27692
                     /gene="ilvY"
                     /locus_tag="RH222_001893"
     CDS             26799..27692
                     /gene="ilvY"
                     /locus_tag="RH222_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV3796926.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27744..29288)
                     /gene="ilvA"
                     /locus_tag="RH222_001894"
     CDS             complement(27744..29288)
                     /gene="ilvA"
                     /locus_tag="RH222_001894"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV3796927.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29291..31141)
                     /gene="ilvD"
                     /locus_tag="RH222_001895"
     CDS             complement(29291..31141)
                     /gene="ilvD"
                     /locus_tag="RH222_001895"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV3796928.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31206..32135)
                     /gene="ilvE"
                     /locus_tag="RH222_001896"
     CDS             complement(31206..32135)
                     /gene="ilvE"
                     /locus_tag="RH222_001896"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV3796929.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32155..32418)
                     /gene="ilvM"
                     /locus_tag="RH222_001897"
     CDS             complement(32155..32418)
                     /gene="ilvM"
                     /locus_tag="RH222_001897"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV3796930.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32415..34061)
                     /locus_tag="RH222_001898"
     CDS             complement(32415..34061)
                     /locus_tag="RH222_001898"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV3796931.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34064..34114)
                     /locus_tag="RH222_001899"
     CDS             complement(34064..34114)
                     /locus_tag="RH222_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796932.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34201..34299)
                     /gene="ilvL"
                     /locus_tag="RH222_001900"
     CDS             complement(34201..34299)
                     /gene="ilvL"
                     /locus_tag="RH222_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV3796933.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34652..36172
                     /locus_tag="RH222_001901"
     CDS             34652..36172
                     /locus_tag="RH222_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV3796934.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36197..36535)
                     /gene="maoP"
                     /locus_tag="RH222_001902"
     CDS             complement(36197..36535)
                     /gene="maoP"
                     /locus_tag="RH222_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV3796935.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36654..37493
                     /gene="hdfR"
                     /locus_tag="RH222_001903"
     CDS             36654..37493
                     /gene="hdfR"
                     /locus_tag="RH222_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV3796936.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37589..37664)
                     /locus_tag="RH222_001904"
     tRNA            complement(37589..37664)
                     /locus_tag="RH222_001904"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37629..37631),aa:Trp,seq:cca)
     gene            complement(37673..37749)
                     /locus_tag="RH222_001905"
     tRNA            complement(37673..37749)
                     /locus_tag="RH222_001905"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37713..37715),aa:Asp,seq:gtc)
     gene            complement(37804..37919)
                     /gene="rrf"
                     /locus_tag="RH222_001906"
     rRNA            complement(37804..37919)
                     /gene="rrf"
                     /locus_tag="RH222_001906"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9275 a        10352 c         9559 g         8790 t
ORIGIN      
        1 actgcgccag aggtaggatt gaaaacgctc tcctgatttt ccaattcatt ttctggataa
       61 ataaataatt tatttttgtc actattattt atgtaatcat cctgtcaggg agagggatct
      121 caattatcaa tgcttaatta cgtcatcatt ttgatgacat gaaaccggag cattttgcat
      181 ggaattttcg ttttcaccca aacttcttgt tgttgctgtc gccgccgctc ttcctctcat
      241 ggccagcgca gcagataccc cgtcaactgc caccgcacgc aaaggctttg ccggatacga
      301 tcacccaaac cagtatccac agaacgctgc aatttattga atttgcacga tcatgtaggc
      361 cggataaggc gtttacgccg catccggcaa tcaaccgcag gcggccacaa aatggccgct
      421 tttttactta ctcaccacca gcaaatgtgc ctgcataatg tcactggccg ggcgaccgtg
      481 cgccagcaaa tcagccattg ctttaagata tggcggtaga tggcggaaat aacgctgata
      541 cccggcacac aaataattca gccccggttt gccgctggca tcgagcatga agcggtgttt
      601 cgggcagcct ccccagcacg cttttaacac gttacaactg cgacactgcg ccggtaattg
      661 cttaaattta tcttcaccaa acacctgctg ttgcggggaa tcgatcattt ctgcaattgt
      721 ttgctgatgc atattcccca gccgatattg cggataaaca tagtgatcgc aggcgtaaac
      781 gtcgccgttg tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat ggcaaaccgc
      841 acccggcgca ccgacaaaat tggcaaacgc ccattcgata ttcatcacga aaatcttgcc
      901 gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaga aattcaccaa atttatcggg
      961 gcgcaccgac cattccgtca gttccccctg aatatcacca ggcgcatgca atttcagtcc
     1021 atcgcgggca gctgtttcat cagccagacg ctcgaccacc ggaataaact ggatgaattc
     1081 gactcccgca tcgcacaaaa aatcataaac ctgcaacggt tgctgcgcgc tggtgcggtt
     1141 gacgcagacc agcacgttat agtcgacatg atgtttttgc agcagcgtca gggcacgcat
     1201 caccagctta tgcgttggtc tgccaccttt ggtcacgcga tattgattgt ggatctcagc
     1261 cggaccgtcc agcgataacc caacaagaaa atgattttct gccagaaatg cacaccattt
     1321 atcatcgagc agcacgccgt tagtctggaa gctgttactt atcttcctgc cagcaccgta
     1381 tttcgcctgt aactttacgg cacggcggta aaaatccagc ccgagtaacg ttggttcgcc
     1441 gccctgccag gtaaaagcca cttcgttttg cggttcgctg gcagcgatat agtgacggac
     1501 atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt aaagggattg
     1561 tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccac tcggtttcgc
     1621 catcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcactcctt gcctgaagtc
     1681 atccttcaat acgttaaggg cgaaatcata ccgtggggca atgagttatt acgggcgttt
     1741 attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa accccgccga agcggggttc
     1801 aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa ccagttgcgc
     1861 taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca attctttaaa
     1921 gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta acacctgaag
     1981 ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata atggggtggc
     2041 taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc aactgagcta
     2101 tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca agcgccggga
     2161 ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag ggcagcgctc
     2221 tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac ggacttccca
     2281 cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt gtgcgttttg
     2341 ctgcttattc ctccagttta tgcgctttcc cgtggcgatt aaggccaaaa actttataaa
     2401 tcgccgtcac cgccagcata aagatgatgc caacaaacag cgacatacgc gtatcttcat
     2461 taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag ttggcccaag
     2521 ggaacaggat tgagcggaac ggatggctgg taatcgccgc tttatgtgca cgccgaaaac
     2581 gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg ctggcactgt
     2641 agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat gagccaatta
     2701 gcagaatagc aatagatact gccacacccg caactggtac gccgtgacgg gaaactttcg
     2761 ccatcgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt ccgcaactgt
     2821 acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg ataatgcccg
     2881 ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa cgggctgccg ttgctgccaa
     2941 tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gcctacgtag aaaatcagga
     3001 tccgccacag cactttgcct acggcactgc gcagcgtcac ctgcggattc ttcgcttcac
     3061 cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata cacagcgcgg
     3121 tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga ttgctaaaac
     3181 caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca atgacaatca
     3241 tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc
     3301 gcaccgccgc cagattcgcc aacgccacca gcgccactgc gatcaatgcg ggtatccact
     3361 gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt tctgagatcc
     3421 ccaccgccat ccacataaac caataagacc aggcggtgag ataaccaaag aacgggctca
     3481 tataacgatg cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaac aacatttcgc
     3541 ccattgaacg catgatgaaa aagacgaata gcccggcgat gatataggcc aacaatacgg
     3601 atggcccggc ccatttcaag gtactggcgg cccccataaa caggccgacg ccaatggtgc
     3661 cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc tctggtttgt
     3721 tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg gttatcgtta
     3781 tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa ataaagcatt
     3841 aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacgaagtga ccactccgtc
     3901 gctttacaaa gagaggaaaa tcagaggttg ccggtgtaat gccagcgtaa ataacgcagc
     3961 aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag ccactccagt
     4021 cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac atcgtaagtc
     4081 ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg catgaagatc
     4141 tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt
     4201 tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc aacgatattc
     4261 acattccatt ggttgcgcag tttagcttca gtttgcgcca gcacttcagg tttaccgccc
     4321 acaagaaata ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg
     4381 gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga acgtacaacg
     4441 ctgatgccat ccgcatattt aaattcggca gcgttaatta actccctgac ctcggcgtta
     4501 tcttcaatgg tcagcatttt ttcagcatta atggcaacca gcgttccctg cttaagctgc
     4561 ccgtcagcaa acagataatc gagggcgtgc tgcatatcac gccaaccaat caactgtaag
     4621 ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct tcaacctgcg
     4681 tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa agccagtaca
     4741 acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagact acgcgtgaga
     4801 caaacgaatc cagcccttca cgtgccagca cgatcatatt gaagatcgcc ccaaagcaga
     4861 aactgtgcaa tatcgcagcc ttatagcgat tagtctcgcg gttgcccagc tcatacagcc
     4921 agtcgaacca tttgatgatc agtccaacca caatcgcccc gagcgggatg aacaacgcgc
     4981 cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg gagtggttat
     5041 tcagcacttc ccaggtaaag tagttggctg agttcagcac catactcggg cgccccggcc
     5101 acagccagga agggataaag acatagaaat cgcggacaat tggagccagg ccctggaagt
     5161 cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggcgag aaggtgtcgc
     5221 gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt ccatagcgtt
     5281 ttaacgccag ccagaacatg ccaacaatcc ccagcacgcc cgccgccgcc agcatccaca
     5341 acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg atgatgatat
     5401 tggcgcgagt gccgccgaca atcatataag tcagcaagcc aaaagcgacc gtgctgacga
     5461 ggaaaaacag ccacgctttg ctgtcctggc gcagaaagta gaccaccagc atcgccggga
     5521 tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggag aagatctgac
     5581 tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg ccgacgctta
     5641 ccagcgcgat gcccatcagg atcacccacg tgagattggt ctccacgcgg ttcatggtaa
     5701 acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg taggtgacat
     5761 agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg caacaagatt tctggcggcg
     5821 cgacaccaac atcaaagcga aataccagca cgctggtcag cgggaagccg aagaaaaagg
     5881 tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa
     5941 accaggtcag cgtggcaata aacagcgtgc agagcagcca gaccacaaac aggccactga
     6001 attgcagcag actcatgcga cctccccggc ggcaatcgcc aacgcccgct gccagccttg
     6061 tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca actggcgctg
     6121 cgcttcaagc acaatatcct cgttgagatc gtcggtagta aacagcaccg gcaaatgctg
     6181 ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa tgcccgcctg
     6241 aatcagtaag cacagcgtac caatgccctg ctggcgggca aaaataaagt aaccgagatc
     6301 gcactgacga agtagcgcca gataggcgtc aaattccagt ttttcgctca gaacttgcag
     6361 attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcaatgt acgcttcgtt
     6421 attaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt gctgatgaac
     6481 ggcacgtaag gcggcgacat gctcattgct gcggtcgccg gagttcccca ccagaatggt
     6541 cattttccct tcacgttgcc gatcgttcgc catcgtattg agagaagggt ccatccgcgt
     6601 cgggaagtac agcagttcgc cccgcacctt tggatgcgtt ttggcaaaaa agctcaaatc
     6661 accgcgggtg gcaaatacat agccgactcg cttttgcgcc aggcgacgta gcgggtaaaa
     6721 aagcttatat ctcaagccac tggaaagctc atacaggtct gccccccaga tatgccagaa
     6781 aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg gattgaactg
     6841 accgtggaaa aaaaatcgct gctgacggtt agcttttgct ttcgcgatga tcgcttccgc
     6901 cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat cacttaagcc
     6961 gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc
     7021 attgaaaaac cgcaaaacgg ttcggttatg gtgagggata tccgatccca gtacgtgaat
     7081 cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag agaaaaatag
     7141 acgatataag ttgccatata tgcctgcgcc gcgcccagtg caccatgcgc agggattagc
     7201 cagtgggcaa ataccatcaa taaagtgaac tggctgactt ccgccagaat ataaaaccgc
     7261 agtgacgctt tggcgatcac cagataacca aagacataag cgcccacttt taacacatca
     7321 cccactaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga caacagcagc
     7381 cagatagcaa aatcacgcag caaccagacg gtaaaactcg ccgccgccac tgccggtaag
     7441 acgaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt tagccgcgac
     7501 aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta ggcatcggaa
     7561 atactgctca ccccttgcca gatccccacc tcatcccagc tatactgcgc tgccagcagt
     7621 ttgcgcatca tgatgtaagc aacaggcaaa gtcaccgacg taatcaacgc catgagcgta
     7681 aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata gcttaacggg
     7741 atgacaccac gtttgatcaa catgatggcg gcaggaatga ccaccagcgc gggaatcagc
     7801 gccagaccca gcaacgcccc ttcataaccg cccaaacggt aactgacgta gtacgcgaga
     7861 acgccaatca agctgccgac aatcagcgat aacgcattac ctgcggcatc gcgaaagcct
     7921 ttcatcagcg ccagtaacag gttgccccag gcgatcccca tttgcaccag cgccactaaa
     7981 cgcaccagcc cctgatagtc ggtattacca aacaatccct ggctgattgg cgcagctgcc
     8041 agcacaaaaa ccagcgccat cagcgtagag aagccaagta ccatcgctga tgaagtgccg
     8101 accacgcggc gcagctgttg gggattatca tggtactggg caacgtattt ggttacaccg
     8161 ttaaagatgc cagccccggc aagcacgccg agcacggtaa tcaactggcg gaaatttgcc
     8221 gccagcccaa gccccgccgg accaaatgac accgccagca acttaccgac cagtaacccg
     8281 gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc caacgacata
     8341 tcaggaaaag tagttcaaca aagtcgcaat taccgtacgc tgattgacgg gcgacaggtt
     8401 gtagaacagc ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc gatcttcacc
     8461 gtggaactca ccaaagtgtt cccccgcagg gcaaccgtgc agcggaatgt aatgaaacac
     8521 cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat caatatcccg
     8581 cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg acggcagctc
     8641 gatacgcccg gctttcgcca gaggcgctaa cgcatcgtag tagttttgcc acagcgccag
     8701 acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc cacaggtatg cagcttgcag
     8761 atcggacatc aaatagctgg agccaatatc gcgccaggta tatttatcga cctgaccacg
     8821 gaagaactgg ctgcggttag tgcccttttc acggatgatc tcggctcgtt cgattaacgc
     8881 tttatcgtta atcagcgtcg cgccgccttc accgcccgcc gtgtagtttt tggtttcatg
     8941 gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt aagtggacat
     9001 cacgccctga gcggcatctt ctaccacaaa caaattatgc tttttcgcca acgccataat
     9061 ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc gcgttttgtc
     9121 ggtgatcgcc gcttcaatca gcgtttcgtc gatgttcatg gtgtccgggc gaacatccac
     9181 aaaaacgatt tttgcgccac gcagcacaaa ggcattggcg gtggagacaa aggtgtagct
     9241 cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca tctccagcga
     9301 agcggtgcag gacggcgtca gtaacacttt ggcgctgcca aaacgttgct ccagccactg
     9361 ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca ttgccgactg
     9421 catatagtcg agttcggttc ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc
     9481 tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat cgtttaagcg
     9541 cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc
     9601 agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg tccagccagc aggccaattc
     9661 gcgcatctgt cgcattgagt tcccgtaaag agacatagcc gcgaatatcg ccggacgccg
     9721 cacgtaaaat cagacattga tgatcaaagg tgccgcgcac ggcattttca atccactgtg
     9781 cataaaagcg actgctggcg tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa
     9841 atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc actacagcac
     9901 cgctatcact ggcattgttc acgggtagcg ccaaatcaac ttcaccttct accagggaga
     9961 atcccagctg ttgcagggca tccagttcac ccgtatttga tgccgcaatt ttggcctgca
    10021 cccgtgacca cggcgctaac gcgtctggcg tcaggagcgg tgcttcagac gtaatgcgca
    10081 cgatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca atactggcgc
    10141 ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc taatgaactg
    10201 gcagcacgct tcacaccctc gtcatcgagc cagccgttac gccaggcgat ctcttccagg
    10261 caggcaatct taaagccctg gcgtttttct actgtctgca caaaggtact ggcttcaatc
    10321 aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag ttcaacggtc
    10381 aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc accacgctcc
    10441 gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata aagcccggtc
    10501 accgcccagt ttgacttcgg ctgttttggc ttttcttcca gcgagatagc gcggaaattg
    10561 tcgtcaaact ccaccacgcc aaagcgttcc gggtccatca cctgatagcc aaaaaccgtc
    10621 gccccttccg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg accgaagaaa
    10681 atgttatcgc ccagcaccag acaagaaggt tcaccgttga ggaaggtttc accgatgata
    10741 aaggcctgcg ccagaccgtc cgggctgggc tgttcggcat attccagctg gataccgaac
    10801 tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt agtgatgatg
    10861 agaatttcgc ggataccggc cagcatcagc accgacagcg gatagtaaat cattggctta
    10921 tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg
    10981 gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgaaaatta gcctttcaga
    11041 cctaaacgct cgccctgata gctgccgtcc tgcacctgct tccaccagct ttcattagcc
    11101 agataccact gcaccgtttt acgcattcca ctttcaaagg tttcctgtgg cagccaacca
    11161 agttcacggg caattttcga agcatcaatg gcataacgca gatcatgccc cggacggtca
    11221 gcgacaaagg tgatcaagtc acgatagtgc gccacgccgt gcggcttgtt cggagccagt
    11281 tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc gttgtgacca
    11341 ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg
    11401 tgatcttcca catacagcca gtcacggatt tgctgcccgt tgccatatac cggcagcggt
    11461 ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa gtggtaagga
    11521 ccgtagttat tcgagcagtt ggtgatcagc gtcggcagac cgtaggtccg cagccaggcg
    11581 cgcaccagat ggtcgctgct ggcttttgac gcggaataag ggctgctcgg cgcatacggc
    11641 gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc gtcggtggag
    11701 atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgtattcca gtaagcccgc
    11761 gccgcttcaa gcaatgtata agtcccgaca atgttggttt caataaacgc tgccgggccg
    11821 tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc tggctgatgc
    11881 tcagtgaata cgcgtgccag ttctgcccga tcgcagatat caactttctc aaaggcaaag
    11941 cgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt cagcttatcg
    12001 accactacca ccgcgtcgct cgtttcgttg atgatataac gcaccagcgc cgagccaata
    12061 aacccggcac cacctgttat cagaattttt ctcatcagcg ccagactcct ttggcatcga
    12121 cgacatactg ctgatggaca ttgtcgccat tgataacttt gaactgacta tgatcgacca
    12181 gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc
    12241 cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagagtt tcgccgctat
    12301 gccactgggc gatcagttca gcgatttcca tcgccgggct ttcgcgcagg tcatcaatat
    12361 tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt ttatcggtag
    12421 cagccaggca atcagccacc gccgctttca cctgatcgat aacccagaac ggtttgtgat
    12481 cgttcacttc gcgcgcggta cggataagcc gcgcctgctg ggggttctgt gccacgataa
    12541 accacggatc aacagcaatg cagtgaccgc ccacgccagg gccaggctga agaatattaa
    12601 cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaatcccc tgatcggcac
    12661 aaatcagcga caattcatta gcaaaagcga tattcacatc gcggaagctg ttttcggtga
    12721 gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg aggaaaattt
    12781 tgtacagttc gctggcgcgg gccgaacaaa ccggcgtcat accaccaatc acgcgatcgt
    12841 ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag taagcaatgt
    12901 tgacgtccgc ctgctcgccc acctgctgtg ggaaagtgag atccggacgc atctctgcta
    12961 accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg atcaccagcg
    13021 cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag gtcatatctg
    13081 gctcatgatc gcctttaaac ggcgtaggta cagcaatcag ccaggcatcc gcttcaactg
    13141 gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg ctcgccaaat
    13201 caggttcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt tggttgatat
    13261 cgacaccaat cacctgtttt tgccgtgagg caaacgcagc tgctgtaggc agcccgatgt
    13321 aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat tattttttaa
    13381 cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg cgcggctcat
    13441 agcttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt
    13501 atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct cagtggtatc
    13561 gcgcatcacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc cgcctgagtc
    13621 ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact cctggggatc
    13681 gatcagaatg acatttttca catgccccag aatgcgattg accggctctc tgacgttcgg
    13741 gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcga tgtctgccag
    13801 cgcgtggcag atttcttcaa agccacgacc gaaactttca cgcctgtgac cggtcaccag
    13861 aatcatcttt ttatcggggt cgataaacgg gtaatttgcc gccagttctg aacgcagcgt
    13921 gtcgctgctc atcacctgat cacgcaccca taacagtgca tcaatgactg tattaccagt
    13981 aatgaagatt cggctatccg caacgttttc acgcagcaag ttttgccggg aagtttcggt
    14041 tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct cttccggcca
    14101 cggcgaatag agatcgcccg tgcgtaaacc agcctcaacg tgaccaacag gaatacgctg
    14161 ataaaacgcc gccaggctgg ttgccagcgt cgttgttgtg tcgccgtgaa ccagcacgac
    14221 gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac aggttatctc
    14281 tgtcaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa tggaaaagag
    14341 tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa
    14401 aaaaggatct tttgccaacg catgcaccaa cggagccatc ttgatggctt ctgggcgcgt
    14461 accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga gcgcctttgc
    14521 gctcgcagca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca ccagccccga
    14581 tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg ctatcgcgtt
    14641 ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga tccagggttg
    14701 gaccaacatt cagggtgttt aacatggccc gattctgatc atagtcgaga tcaaaggccg
    14761 gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca agcaggaaca
    14821 tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa atattatgct
    14881 gctcagcaat tttcagcgcc tgctcaatgc tgttcatccg gcggtcgtag atggctttcg
    14941 ccacctcttc ctgacgcttc acctgagctt tcatctggat ggtgcgtgcc gcccatgcgc
    15001 ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca acatactgac
    15061 gtaacaggtt attagcgtca ggcgcggttt cggcaataag cttcacgctg tcattgaccg
    15121 cgcgggtaaa gtctccgggg ataaactgga tgttgttaat catttcatcc agcaacgccg
    15181 catcggcttt gctgttgccc accatccgct gtttgtaata gtcggtttgc agccagaact
    15241 ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct tcgtccatga
    15301 ccgatggttg atcggcagaa gccatgtttg aacggacatc caggttacgc aaaaattgct
    15361 gctgcgagta atatcccccc agcatattca ccgttggacg atcggtaatc gccgtcgagc
    15421 tccactcctg acgagcaaaa aaagtatacg ccagcgcgat taacgcaaac gccagcccca
    15481 tgccaataat ccatagcttc ccagcccaca aggtacgaaa caacccacga atatccagtt
    15541 cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca tcctcattta
    15601 tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta cgcgcttaat
    15661 aaagcgagca actttccagg cacgcttaat gcaatatcca tagaggaaga atgctagcaa
    15721 aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca gcacgccaat
    15781 ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctgacggg aagtaaaccc
    15841 ggcacgcatg atcaaatggt gaatatgctg acggtcagga gagaatgggc tcatgccttt
    15901 acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga ttatccacaa
    15961 agcggtaacc gggctgatgg gatgggtttt gccctgggtc gtttcgagca ggatccagat
    16021 aacggtaaaa ccaatcagcg tactgcccgc atcacccata aagactttgt agcggcgacc
    16081 caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg caaagcacca
    16141 gattgcgagg ctggtttgcc cgtcgaacca caaaatcata ccgattgctg caaacgagac
    16201 gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg cattaatggc
    16261 cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca tctcccagga
    16321 gccaaagata taacccaggc tactgagata aagtttgccg aacaccatca taacaatgcc
    16381 aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt catccagcgc
    16441 gccaatgaaa acaagcacac cggcacaagc gagatagaga gatgcatgcg gaatatagta
    16501 atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc caacgagagg
    16561 tatcaatccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga ctttttttgc
    16621 caccttacgg gcaaaaaaca acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc
    16681 agtactcact gtcagtaaat tcacaatgca tgctctcaga gaaaattatt agcagaagta
    16741 taaccacgaa gacctttatt cagaagggaa agtccgttcc gaacccacca gtcctgtgca
    16801 aaaattaacc atttgatatg caaattgcat aacatgccag caaattccag gaattttcct
    16861 aacatcgcca gcgcggcatt aagattacag atataaaagc aaaacgccac gtaaacacgt
    16921 ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa atcgtcattg
    16981 gtcttggtca tggccagttt attaatgagg aattccattg catcgatttc gcccatcggg
    17041 tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtcgt gagcagctct
    17101 tctttacggg taccggaacg gttgtagtcg atagccggga agacgcgttt ttcagcgatc
    17161 ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta aataacttcg
    17221 tccattttag aaccggtatc gataagcgcc gtcgcgataa tggtcaggct gccgccctct
    17281 tccacgttac gcgccgcacc gaagaagcgt ttcggacgat gaagggcatt ggcgtccaca
    17341 ccaccggtca acactttacc tgacgccgga acaacagtgt tgtaagcgcg cgccagacga
    17401 gtgatggagt cgagcagaat gataacgtct ttcttgtgct caaccaggcg tttggccttc
    17461 tcgatcacca tttccgcaac ctgaacgtgg cgagatgcgg gttcgtcaaa ggtagaagca
    17521 acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg ttcgtcgatc
    17581 agcagaacca tcagcacaca atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc
    17641 agcagcatag ttttaccggc tttcggcggt gccacaatca gaccacgctg accacgaccg
    17701 ataggtgatg ccagatccag tacgcgagcg gttaaatctt ccgtagaacc gttaccacgt
    17761 tccatacgca gacgagagtt tgcgtgcagc ggggttaagt tctcaaagag gattttgttg
    17821 cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc aaaatagcgt
    17881 tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg gaggttgaaa
    17941 cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta ggagctgtct
    18001 gcggaacgga ggaaaccaaa tccatcctgc aatatctcca gtacgccatc accaaagata
    18061 tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg cttacgcata
    18121 cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga aaccggcgta
    18181 ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt aaatctcgaa
    18241 cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca tcgactcatg
    18301 tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga cggattgagt
    18361 gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt tggaaattac
    18421 aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta gcatgactca
    18481 cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct tacgccaggt
    18541 tagcgtcgag gaactctttc aactgacctt tagacagtgc acccactttg gttgccgcca
    18601 cttcaccgtt tttgaacagc agcagagtcg ggataccacg gatgccatat ttcggcgcag
    18661 tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga tattcgtcag
    18721 cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct gcccagaaat
    18781 cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg tcagtcaggt
    18841 gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt tggtttcgtt
    18901 gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg ctttcgtaaa
    18961 gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca gaacagaagt
    19021 tttccgactt cgccctgcat ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata
    19081 actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg
    19141 ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg tttcattatc
    19201 ttctctctca tcctgcgatt gccgatcgca aggtgaatca accacgtgcc ttaattatgg
    19261 caccgacgcg tgaacttgcc gtgcagatcc atgccgacgc agaaccgctg gcggaagcta
    19321 ccggcctgaa gctgggtctg gcttacggtg gtgatggcta cgacaaacag ctgaaagtgc
    19381 tggaaagcgg cgttgacatt ctgattggca ctacggggcg tttaattgac tacgccaagc
    19441 agaaccacat taacctcggt gccattcagg tggtggtact ggacgaagcc gatcgcatgt
    19501 acgatctggg ctttattaaa gatatccgct ggctgttccg ccgtatgccg cctgcaaacc
    19561 agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg
    19621 agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg ggccaccgta
    19681 ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg caaacgctga
    19741 tcgaagaaga gtggccagac agagcgatta ttttcgccaa taccaaacac cgttgtgaag
    19801 agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca ggcgatgtcg
    19861 cacagaaaaa acgtctgcgt attcttgatg aatttacccg tggcgatctg gatattctgg
    19921 ttgccaccga cgttgccgcg cgtggtttgc atattccggc agtgacgcac gtctttaact
    19981 acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca ggtcgcgcag
    20041 gcgcaagcgg tcactctatc agcctggcgt gtgaagagta tgcattgaat ttgcctgcta
    20101 ttgagaccta tattggtcac tcaattccgg taagcaaata caatccggac gcattgatga
    20161 ccgatctgcc aaaaccgctg cgcctcacgc gcccacgtac aggcaatggt ccgcgtcgta
    20221 ctggcgctcc gcgtaatcgt cgtcgttcag gttaaataaa atgtcatact caaaatcatt
    20281 caaactgtat caagatggca agagaatgaa tcccgatgag catactgtag taagtgattc
    20341 aggtgaaaga atgcagccaa cacagagaca gattgaagga tgaagagtat gggttccacc
    20401 tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct ggttgtgcgc
    20461 gaggtggctg gaagcatcca gacgctgacg cgaataaaac gcaaagtgcg tctggctgct
    20521 ggcctgaata gcgaaaatgc cctgtctaat gaagcaatgg agcgcggttg gcaatgtctg
    20581 cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc aaattcgcgt tgtcgctacg
    20641 gcgacattac gtcttgccgt caatgcgggt gattttattg ccaaagcgca ggaaatcctc
    20701 ggttgtccgg tacaggtaat cagcggtgaa gaggaagcac gtctgattta tcagggcgtt
    20761 gctcacacaa ctggcggtgc cgatcagcgc ctggtggtgg atataggcgg tgccagtact
    20821 gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc gatgggctgc
    20881 gtcacctggc tggaacgcta ttttgccgat cgtaatctgg ggcaggaaaa ttttgatgct
    20941 gcagaaaaag cggcacgcga agtgttacgt ccggttgccg atgaattacg gtatcacggc
    21001 tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt tacaggaaat catgatggca
    21061 caggggatgg atgaacgcat taccctggaa aagttgcagc aattgaaaca gcgagccatt
    21121 cattgcggtc ggctggagga actggagatt gacgggctga cgctggaacg tgcgttagtg
    21181 ttcccgagtg gtctggcgat cctgatcgcc atttttaccg aactgaatat tcagtgtatg
    21241 accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gcatgttgca tctggccgtc
    21301 gagcaggata ttcgcagccg tacgctgcgt aatattcagc gccgctttat gatcgatatt
    21361 gatcaggcgc agcgcgtagc caaagttgca gctaacttct tcgatcaggt ggaaaacgaa
    21421 tggtatcttg aagcaataag ccgcgatttg ctcatcagcg cttgtcagct tcatgaaatc
    21481 ggcctgagcg ttgacttcaa acaagcgccg caacacgctg cttatctggt gcgtaacctg
    21541 gatcttcccg gttttacccc tgcacagaaa aaactgctgg cgacgctact gctcaaccag
    21601 actaacccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccacc gcgcgtcgca
    21661 gaacaactct gccgtttact ccgcctggcc atcatttttg ccagccgtcg tcgtgacgat
    21721 ttagtgccag agatgacatt acaggctaac catgaactgt tgaccttgac gcttccgcaa
    21781 ggttggctaa cccaacatcc gctgggtaaa gagattattg ctcaggaaag ccagtggcag
    21841 agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg cgatgctgac
    21901 gcatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct ttcagattcg
    21961 ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg cgctcctgtt
    22021 cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg gctcggctcc gggcgcacca
    22081 gctcgccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc tgggcgcggg
    22141 taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg atgctgctct
    22201 ggtgcggcaa aaacccttct tccataccga ccatgtagac ataaggaaac tccagccctt
    22261 ttgacgcgtg cagagtcatc agttgcacct gatccagctc ttcttcactc tcaccacgct
    22321 ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc ggctcatcca
    22381 gttcactgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg ttcttcatgc
    22441 gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca taatccatgc
    22501 catggatcag atcacgcacc gcagcaatcg gctcccgctc cgccaggcgc tggatttctg
    22561 ccaaccagtg agtgaagcgg gtcaatgctt cataaccacg tccgctaagc gtctgactca
    22621 ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc cactcaccca
    22681 gctttttcag cgtcgccggg ccaatctctc gcttcggcgt gttaacgata cgcagaaatg
    22741 cgctgtcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc ttgatttcag
    22801 gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg gatgcggttt tgcatcagga
    22861 atttttcaaa cacccgcgac tgatggttac cgcgataaag aatggcgtaa tctttgtact
    22921 gcgttttatt gacgaagtga tgggcgatca gctcgccagt aacgcgctcg gcttcatgtt
    22981 cctcgttatt cgcgcttaaa acttttagct ctgtgccgta acccagttcg gagaacagac
    23041 gcttttcaaa gacgtgcgga ttattggcga tcaggatgtt cgccgctttc aaaatacgtc
    23101 cggaagagcg gtagttctgc tcaagcttaa tcaccttcag cgccggaaaa tcctgactca
    23161 gcagcaccag gttttgtgga cgtgcgccgc gccaggagta gatcgactgg tcgtcatcgc
    23221 ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagctca tactggctgg
    23281 tgttggtgtc ctgatactca tccaccagca gatagcgaat tttgttctgc cagcgctcgc
    23341 ggacttcttc attgcgttgc agcagcaacg tcggcaataa aatcagatca tcgaagtcga
    23401 gaacgttaca ggctttcagg tgtgcatcat acagcccata acaatgggca aaaatacggt
    23461 cccgctcgcc aatcgcactg gctgccgcct gggacggtgt tttgagatcg ttcttccagt
    23521 tagagatggt cgaaatcaac tgttgcagga gaactttgtc atcttcaatc agcccttcgg
    23581 tcaactcttt gagcaacgcc agctgatcgg tatcgtcaaa cagcgaaaag ttcgccttca
    23641 tccccagcgc cgcatactca cgcttgatga tatccagccc cagcgtgtgg aaggtggaga
    23701 tcatcagccc acgcgcctct ttgcgaccca gcgtctgccc gacacgctct ttcatctcgc
    23761 gcgccgcctt attggtaaag gtcaccgccg cgatatgtcg cgcctgataa ccgcaaccgc
    23821 ggatcagatg agcgatttta ttggtgataa cacgagtttt accggaaccc gcgcccgcca
    23881 gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg tttagacgca
    23941 taggtgtatt gctcaatctt cgaacggggg gaggattgta gcagaatcgc atttgaatgt
    24001 gaccgcgtgt aagccgctaa ccggttggct gcggatgttt aaatcagaga gagaagaccg
    24061 aaaccggagt tcatcgtgaa tccattagac ttagaaaata tcgggtcgta acgttgtgtt
    24121 attacttttt cagagtttac taaggagagg tgtactcgtg gcgattaatt tcagtcctaa
    24181 ggttggtgaa atactggaat gcaactttgg taactatcct gttagccaga atggacagtt
    24241 cagcaccacg tattacgatg gccgcattcc tcctgaaatg ataaaaaaca ggttagttgt
    24301 cgtgctcaac ggaaaaatta atggcaacgc atgtatcgtc attccattat caaccactcg
    24361 cgatcatgac aagttaaatc gtggaatgca tgttgaaata gccagtaatg ttatcaatgg
    24421 cctgcaattc tttgatcagc agattcgttg ggccaaagcc gacctggtac aacaagtcag
    24481 tcgaaaccga ttaaatagag caaggactta cagagggtat cttaatcaat gtttgccaca
    24541 tgagttggtt gcagacatac agagggcggt catcaagtca atcaatgcca tatcgctcat
    24601 aaattgacaa taaaatcacc atccccgata atgactttgt agcccataga ggccctaagt
    24661 tttctcccct tgagggacct gcgaagcccg gattttccgg gcttttgcca tatatgtacc
    24721 agcccctccc tgactgacaa ttacgttagc atctgcggat catgctaaaa tcgccgcctg
    24781 acaattattc actcaaggca cgatcatggc aaaaacagca gcagcactgc atatccttgt
    24841 aaaagaagag aaactggctc tggatcttct cgagcagatt aagaacggtg ccgatttcgg
    24901 caagctggcg aagaaacact ccatttgccc atcaggcaaa cgcggcggtg atttaggtga
    24961 attccgccag ggtcagatgg ttccggcgtt cgataaagtg gttttctctt gtccggtact
    25021 ggagccgacc ggcccgctgc acacccagtt cggatatcac atcattaagg tgctgtaccg
    25081 caactaatag caaggccttc tccaggagaa ggccttgagt gttttctccc tctccctgtg
    25141 ggagagggtc ggggtgaggg catcagcgcg cacttaaccc gcaacagcaa tacgtttcat
    25201 atctgtcata tagccgcgca gtttcttacc tacctgctca atcgcatggc tgcgaatcgc
    25261 ttcgttcaca tcacgcagtt gcgtgttatc taccgcgcct tccggaatag ctttacccaa
    25321 gtcgcccggt tgcagctctg ccataaacgg tttcagcaac ggcacacaag cgtaagagaa
    25381 cagatagtta ccgtactcag cggtatcaga gataaccacg ttcatttcgt acagacgctt
    25441 acgggcgatg gtgttggcaa tcagcggcag ctcgtgcagt gattcataat atgcagactc
    25501 ttcaatgatg ccggaatcga ccatggtttc gaacgccagt tcaacgcccg ctttcaccat
    25561 cgcgatcatc agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg
    25621 cggcgcggtt tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc
    25681 atcgttggcc cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat
    25741 atgtttctgg aacaggggtg ccatgatctc tttcagctgt tcagacagcg cgtaagcacg
    25801 cagtttcgcc gggttagaga gacggtccat catcagggtg atgccgccct gcttcagcgc
    25861 ttcggtgatg gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc
    25921 ctcttccacc agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat
    25981 ggtttgctca cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc
    26041 cgcacggtga ccaccggttg ctgccgccca tgctttagcg atcgccatac cttcgccttt
    26101 cggatcgttt tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc
    26161 ttcacgcact tcggtgccag ggcatttcgg cgcaaccatg actacggtga tatctttacg
    26221 gatctgctcg cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc
    26281 tttcatcagt ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt
    26341 aaccaccaga tccgcctgcg ggatcagttc ttcgtaagta ccaactttaa aaccgttttc
    26401 ggtcgcttta cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga
    26461 gatatcgaga ccagaatcac gcatgttcag gccctggttc agcccctgtg cgccacagcc
    26521 gacgataacc acttttttac cctgaaggta gctcgcgccg tcggcgaatt cgtcgcgccc
    26581 cataaagcga catttgccca gctgtgccag ctgctggcgc aggttcagtg tattgaagta
    26641 gttagccata gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag
    26701 attgaattca ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca
    26761 ttgcaatttc tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa
    26821 ccttcctgca tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta
    26881 gcccatccac gctctcacgg cagattcagc gcctggaaga agatcttggt cagccgctat
    26941 ttgtgcgcga taaccgcacg gtaacgctga ccgaagcggg cgaagagtta cgcgttttcg
    27001 cccagcaaac attgttgcag tatcagcaac tgcgccacac catcgatcag caagggccgt
    27061 cgctctctgg cgagttacat attttctgct cggtgaccgc cgcttacagc catctaccgc
    27121 cgattctcga ccgcttccgc gcggaacacc cgtcggtgga gatcaaactc accactggtg
    27181 atgcggcaga tgcaatggaa aaagtggtca cgggagaagc ggatctggcg attgccggga
    27241 aaccggagac gctacccggc gctgtggcgt tttcgatgct ggagaatctg gcagtagtgc
    27301 tgattgcccc cgcgctgccc tgcccggtgc gtaatcaggt atctgtcgaa aagcccgact
    27361 ggtcaacggt gccgtttatt atggccgatc aggggccggt acgccgccgc attgaactgt
    27421 ggtttcgacg caataaaatc agcaacccga tgatttacgc cacggttggc gggcatgaag
    27481 cgatggtatc gatggtggcg ctcggctgtg gtgtggcgct gttgccggaa gtggtgctgg
    27541 aaaacagccc ggagcctgta cgcaaccggg taatgatttt agatcgcagc gatgagaaaa
    27601 cgccgtttga actgggcgtg tgtgcacaaa aaaagcggct gcatgagccg ctgattgagg
    27661 cgttctggag gattttgccg aaccacaaat gacgttgtcg cgcgggtagg cctgataagc
    27721 gaagcgctat caggcatttt tccctaaccc gccaaaaaga acctgaacgc cggattattg
    27781 gtttcgtcgt ggcaatcgta gcccagctca tttagccggg tttcgaaatc cggttcatgg
    27841 tcgccgagtt cgaacgccgc cagtacgcgc ccgtagtcgg tgccatggct gcgatagtgg
    27901 aacaaagaaa tgttccagtg cgtacccagc gtgttgagga agcgcagcag cgcgcccggt
    27961 gattccggga attcgaagct gtagaggcgt tcctgcaacg gatgtgacgg acgcccgccg
    28021 accatatagc gcacatgcaa cttcgccatt tcgtcgtcgg agagatccac cacgctgtag
    28081 ccgccgtcgt tgagcatctg caaaatttct ttgcgctctt cgaggccgcg gctcaggcgc
    28141 acgccgacaa agatgcaggc gtttttggca tcggcaaaac ggtagttgaa ctcggtgacc
    28201 gatcgcccgc caagcagttg gcagaatttg aggaagctgc ctttttcttc cggaatggtc
    28261 accgccaaca acgcttcacg ctgttcgccc agttcgcagc gttccgagac gtagcgcagg
    28321 ccgtggaagt tcacgttggc accggaaaga atatgcgcca gccgttcgcc gcgaatgttg
    28381 tgctgggcga tatatttttt cattcccgcc agcgccagcg cgccagaggg ttccgccacc
    28441 gcgcgcacat cttcgaataa atccttcatc gccgcacaga tcgcatcact atcgacggtg
    28501 atgatgtcgt cgagatactc ctggcataaa cggaaggttt cgtcaccgat gcgttttacc
    28561 gctacgcctt cagcaaatag ccctacgcgc ggcagatcaa ccggatgacc cgcatccagc
    28621 gctgctttca ggcaggcgga gtcttccgct tctacggcga tcactttgat ttgtggcatc
    28681 agttgtttga tcagcaccgc aacgcccgcc gccagaccgc cgccgccgac tggcacaaat
    28741 acgcggtcga gatgggcgtc ctgctggagc agttccagcg ccagcgtgcc ttgcccggca
    28801 atcaccatcg gatggtcgaa cggcggcacc caggtgaacc cctgctgctg tgacagttcg
    28861 atcgctttag ctttcgcttc gtcaaagttc gcgccatgaa gcagcacttc gccgccgaat
    28921 ccacgtaccg catcgacttt gatgtcggcg gtggcggttg gcataacgat cagggccttc
    28981 acgcctaacc gcgcagaaga aaacgcgacg ccctgcgcgt ggttacccgc agaagcagtg
    29041 atcacgccgt gcgctttttg ttcttccgtc aggcccgcca tcatggcgta tgcgccgcgc
    29101 agcttaaagc tgtgcactgg ctggcgatct tcgcgcttca ccagaatgac gttatcaaga
    29161 cgcgacgaca gtttttccat tttttgtagc ggcgtaacct gcgccgcctc gtaaaccggc
    29221 gcgcgcagca ctgctcttaa atattcggca ccttccggag caccggacag gggttgcgag
    29281 tcagccatta ttaacccccc agtttcgatt tatcgcgcac cgcacctttg tcggcgctgg
    29341 ttgccaggct ggcgtaggcg cgcagggcaa aggaaacctg acgttcacgg tttttcggcg
    29401 tccaggcttt gtcaccccga gcttcctgcg cttcacgacg cgccgccagt tcggcatcgc
    29461 ttacctgtaa ctgaatgcca cggttcggga tgtcgatagc gatcaggtca ccatcttcaa
    29521 tcagaccaat gctgccgccg cttgccgctt ccggtgagac gtggccgatg gaaagaccag
    29581 aggtgccgcc agagaaacga ccgtcggtga tcagcgcaca ggctttaccg agacccattg
    29641 atttcaggaa gctggttggg tagagcattt cctgcatgcc agggccgcct ttcgggcctt
    29701 catagcggat caccaccaca tcgcccgcga caactttgcc accgaggatc gcttctaccg
    29761 cgtcgtcctg gctttcgtac actttcgccg ggccggtgaa tttgaggatg ctgtcatcga
    29821 cgcccgcggt tttaacgatg cagccgtttt ctgcgaaatt accgtagagc accgccaggc
    29881 cgccgtcttt gctgtaggcg tgttccagcg agcggataca gccattggcg cgatcgtcat
    29941 cgagagaatc ccaacggcaa tcctgcgaga atgcctgtgt ggtacgaatg cctgcaggac
    30001 ctgcgcggaa catatttttt accgcgtcat cctgggtcag cataacgtcg tattgttcca
    30061 gcgtttgcgg caacgtcagg ccaagtacgt ttttcacatc acggttcagt aaccccgcgc
    30121 gatccagttc gccgagaata ccgataacac caccagcacg gtgaacatct tccatatggt
    30181 atttctgggt gctcggcgca actttacaca gctgtggaac cttgcgggaa agcttatcga
    30241 tatcactcat ggtgaagtcg atttccgctt cctgcgccgc cgccagcagg tgaagtacgg
    30301 tgttagtcga tccacccatc gcgatatcca gcgtcatggc gttttcaaac gccgccttac
    30361 tggcgatatt acgcggcagt gcactttcgt cgttttgctc gtaataacgt ttggtcaatt
    30421 caacaatgcg tttaccagca ttaaggaaca gctgcttacg gtcggcgtgg gttgccagca
    30481 gcgagccgtt gcccggctgc gacaggccca gcgcttcggt caggcagttc attgagttag
    30541 cggtaaacat cccggagcag gaaccgcagg ttgggcaggc ggaacgttca acctgatcgc
    30601 tctgggagtc agaaactttc gggtctgcgc cctggatcat cgcatcaacc agatcgagtt
    30661 tgattatctg atcggacagt ttggttttcc cggcctccat cgggccgccg gaaacaaaga
    30721 tcaccggaat attcaggcgc agggaagcca tcagcatccc cggggtgatt ttgtcgcagt
    30781 tagagatgca gaccatggcg tcggcgcagt gggcgttgac catatactca acggaatcag
    30841 cgatcagttc gcgagatggc agtgaataaa gcatcccccc gtggcccatg gcaatcccat
    30901 catccaccgc aatggtgttg aactctttgg caacgccgcc agccgcttca atttgttcgg
    30961 cgaccagttt accgagatcg cgcagatgga cgtgacccgg tacaaattgg gtgaacgagt
    31021 tcacaaccgc gataatcggc ttaccgaaat cggcgtcggt cattccggtg gcgcgccaca
    31081 gcgcacgagc acccgccata ttacgaccat gagtggtggt ggcggaacgg tacttaggca
    31141 tactttattt actcccagtg tctgtctcgt aaatgggacg gtgcgtgccg tcccattttt
    31201 tgtatttatt gattaacttg atctaaccag ccccatttat cttcggtttc gccagtgaag
    31261 aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc cacaacggcc ttcgccaacc
    31321 tgaataccgt ctacgctgcg cactggcgtg atttctgccg ccgtaccgga cataaacact
    31381 tcatccgcca ggtacaggga ttcgcgcgac agcacctgct cacgtacttc aattcccagc
    31441 tctttcgcca gtttgatgat ggcatcacgg gtaatacccg gcagcgcgga ggaggtgaac
    31501 ggtggggtga acagcacacc atctttcact tcaaacaggt tttcgcctgc gccttcagag
    31561 atataaccgt tcacatccag cgcgatacct tcctgataac cgtggcggcg cgcttcgcta
    31621 cccaccagca gggaagagag gtagttacca ccggcttttg ccgccgtcgg gatggtgttt
    31681 ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc cctgctccag cgcttctgcg
    31741 cccagatacg ctccccacgg gaaagcagcg ataatcacgt cggttgagta tcccgctggc
    31801 gggtttactc ccatgccaac atcaccgacg aagatcagcg gacggatata ggcgctggtg
    31861 agattatttt tgcggatcac gtcacgacaa gcttccatca gctcatcaat gctctgcgaa
    31921 accgggaagc gatagatttt ggcggagtca tgcagacgct gcatatgctc acgatggcgg
    31981 aatacaaccg gtcctttgtg cgagtcgtag caacggatgc cttcaaaaac cgaagtgcca
    32041 tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt cccagcgaac catctcccca
    32101 ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta tattcctttt gcgctcaggc
    32161 gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca acgtgtgcga cgtccaccag
    32221 tttatttaac tgactaaaca gtaagtcgac cgaccgtggg ctggcaacgg tcaattcgat
    32281 atttatattt tgtgcatcgc tggcggcggc catattcatt gagcagacgt ggaaaccacg
    32341 atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga ttgaagcgag ccgatacatt
    32401 gacctgatgt tgcatcatga taatttctcc aacatttctg aattactggc accaggcggc
    32461 accagcggcc agacgttctc aagttcgtcg attgagacat gaagcaggta tggcccatca
    32521 ctgttcagca tggtgtcgag tgccgcttca acctggtctt tacgggtgat gtgttggcca
    32581 gggatgccga aggcgctggc taacatgaga aaatcggggt tatcggtaag ggtggtttcg
    32641 ctatatcgtt cctggaaaaa cagttgctgc cattgtcgaa ccatccctaa ccgttggtta
    32701 tcgagtaaga cgattttcaa cggtaactgc ttacgtttta cggtgcccag ctcttgtaca
    32761 ttcatcatga aagagccgtc accggagata cagacgacgg tatcgttcgg tcgcgcgact
    32821 tgcgcgccaa ccgccgccgg taaaccgaaa cccatggtgc ctaagccgct ggaggtgatg
    32881 aaattttccg ggcgagtatg ggcgatgtgc tgcgcagccc acatctggtg ctgccccaca
    32941 tctgtggtca cgacgcaatc cgcaggttta cgatccgaca gttgttttaa caacaatggc
    33001 gcgtagatag cgtcaccggg atggtcgtaa cgccaggcat gttcatcacg cagctgcgcg
    33061 cagtgtagct gccagtcatt gatatttaac ggctgctgta atgctggtaa cagagcattt
    33121 aaatcacctt gtaatgccac atgtgcctga cgcagcttgt tcatttctgc cgggtcgata
    33181 tccatatgga taacactggc gtgtggtgcg aaggtgttca gtttgccggt cacccggtca
    33241 tcaaaacgtg cacccacggc gatcagcaag tcgcactcct gcaccgcgaa gttcgccgct
    33301 ttggtgccat gcattcccag catgcccaga tagtacggat aatctgcttc aactgcgccc
    33361 agccctttca gcgtgcaggt ggcaggcatt tttgtggtag cgagaaattc tcgtaaagca
    33421 ggaactgcct gcgccatacc cacgccacca ccaacgtaca gcatcggttt ttgcgctttt
    33481 gccagcatct ggcgcgcttg ctcgacttcg gcatgtggga aagtcacttc gttttcaacg
    33541 gtggtgaacc acggttccag gtcaccgctg gctagctgga tatcttttgg gatatcgacc
    33601 agaaccggac caggacgacc tgagctggca acgtcgaatg cttcagccat gatgcgcggc
    33661 aattcttcca gcgactgcac cagaaagctg tgcttggtac aggctaacga caatcccagg
    33721 acatccactt cctgaaatgc gtcagtgccg ataaacggtg cggacacttg accggtgatg
    33781 gcaacaacag ggatagaatc taacagtgcg tccgcaagcc cggttatcag gttggttgcg
    33841 cccggaccag acgtggcgat acatacgcca gttttgccgg tagcacgggc ataaccgata
    33901 gccgccattg ccgcaccctg ctcatgtcgg cacagcaagt gctccacgcc gccgtcatac
    33961 aatgcatcgt aaaccggcat aattgcgcca cccggataac cgaaaacggt gttcacaccc
    34021 tgtgcccgca acgcatgtac cacccactgt gcgccattca tagttagttc cccgtcctga
    34081 atcttgagaa acagaatttt gtgctgttat tcattgtctg ctcctcggtt atgtttttaa
    34141 ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt agttcgttaa ggcttgatct
    34201 ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg ataataatca ccaccacgct
    34261 aatcacgacc aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg
    34321 ttcgaaggaa tgcctaaaga gttaccatag gttttgtaaa tagtacaaga tattttttgg
    34381 ccgattttta tgatgcgttt aataatttca atataaattg ttgtttttta aaaataaaat
    34441 agtgagtgaa aaaaattcaa tttcagtatg aatgttgttc ccttcctcgt agttcatcct
    34501 ttgtccgctg catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca
    34561 caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct
    34621 tatgcacata atggtgactc aaggagggct tatgtcactg tcaattgttc atacccgcgc
    34681 agccctggga gtaaatgcgc ccccgatcac tgttgaggta catatcagta aaggtctacc
    34741 cggcttaacg atggtgggct taccagaaac aacggtaaaa gaagctcgcg atcgcgtgcg
    34801 cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaacctggc
    34861 tccagccgat ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct
    34921 ggcggcctca gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact
    34981 ggctcttaca ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat
    35041 taagtcgggc agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa
    35101 cggtgaagga tgcctgatag ccgatcatct gcaagctgtc tgtgcgtttc tggaaggtaa
    35161 gcacgctctc gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct
    35221 cagtgatgtt gtcggtcagg aacaaggaaa gcgaggactg gaaattaccg ccgctggcgg
    35281 gcacaacctt ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat
    35341 taatggcctg ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag
    35401 tctggtaaat gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca
    35461 tcacagtgcg tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat
    35521 ttcgttggcg cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac
    35581 actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc
    35641 aaaaataacc tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg
    35701 acattatcag ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg
    35761 gctctcgggg ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc
    35821 cggcattttg agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt
    35881 aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag
    35941 tccggaaata cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaaggaac
    36001 actgatccat ctggggttat cgattcgtgc ctggcagcgg ttattgaaag ttgcacgaac
    36061 cattgctgat attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag
    36121 ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg
    36181 catttcgccc tttttattaa tcatcagaat cggtgtagtc ttcagcacct tcaacctgcg
    36241 gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg
    36301 accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga
    36361 gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac
    36421 catgacgctc aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac
    36481 gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc
    36541 acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga
    36601 gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc actgtggata
    36661 cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg
    36721 aatcgctcta tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac
    36781 tgggtgtgaa ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa
    36841 aactactgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgt aaggaggtgg
    36901 cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta
    36961 tgcttaatca gtggctggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt
    37021 tcgaagcgcg aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg
    37081 atcttcttat taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctgggat
    37141 atttcacttt agcgctttat accagtgccc cttcaaaact aaagggagat cttaattatc
    37201 tgcgacttga gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg
    37261 aagtgcccat tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta
    37321 atggttgcac ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg
    37381 tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa
    37441 atacgttgat tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatatgaat
    37501 ggctggcaag gatgccggta gaaggattta cttcggagag ggttatttca gataaaaaaa
    37561 atccttagct ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca
    37621 cccggttttg gagaccggtg ctctaccaat tgaactacgc ccctaattag ggtggcggaa
    37681 cggacgggac tcgaacccgc gaccccctgc gtgacaggca ggtattctaa ccgactgaac
    37741 taccgctcca ccgaattctt ttacaaccac cggttttatg accggcttac tgcttaattt
    37801 gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg
    37861 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctacg gccgccaggc
    37921 aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaa
//
LOCUS       DAPHJE010000038        37609 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPHJE010000038 DAPHJE010000000
VERSION     DAPHJE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.038"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            223..1596
                     /gene="aroP"
                     /locus_tag="RH222_001907"
     CDS             223..1596
                     /gene="aroP"
                     /locus_tag="RH222_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV3796937.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1639..2493)
                     /gene="ampE"
                     /locus_tag="RH222_001908"
     CDS             complement(1639..2493)
                     /gene="ampE"
                     /locus_tag="RH222_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV3796938.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2490..3041)
                     /gene="ampD"
                     /locus_tag="RH222_001909"
     CDS             complement(2490..3041)
                     /gene="ampD"
                     /locus_tag="RH222_001909"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV3796939.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3129..4022
                     /gene="nadC"
                     /locus_tag="RH222_001910"
     CDS             3129..4022
                     /gene="nadC"
                     /locus_tag="RH222_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV3796940.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4225..4665
                     /gene="ppdD"
                     /locus_tag="RH222_001911"
     CDS             4225..4665
                     /gene="ppdD"
                     /locus_tag="RH222_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV3796941.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4675..6060
                     /gene="gspE"
                     /locus_tag="RH222_001912"
     CDS             4675..6060
                     /gene="gspE"
                     /locus_tag="RH222_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV3796942.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6050..7252
                     /gene="hofC"
                     /locus_tag="RH222_001913"
     CDS             6050..7252
                     /gene="hofC"
                     /locus_tag="RH222_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV3796943.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAILHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7287..8330)
                     /gene="guaC"
                     /locus_tag="RH222_001914"
     CDS             complement(7287..8330)
                     /gene="guaC"
                     /locus_tag="RH222_001914"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV3796944.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGYEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8486..8530)
                     /locus_tag="RH222_001915"
     CDS             complement(8486..8530)
                     /locus_tag="RH222_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796945.1"
                     /translation="MIIKTLKMSTRKRN"
     gene            8555..9175
                     /gene="coaE"
                     /locus_tag="RH222_001916"
     CDS             8555..9175
                     /gene="coaE"
                     /locus_tag="RH222_001916"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV3796946.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9175..9918
                     /gene="zapD"
                     /locus_tag="RH222_001917"
     CDS             9175..9918
                     /gene="zapD"
                     /locus_tag="RH222_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV3796947.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9928..10125
                     /gene="yacG"
                     /locus_tag="RH222_001918"
     CDS             9928..10125
                     /gene="yacG"
                     /locus_tag="RH222_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV3796948.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10341..10730)
                     /gene="mutT"
                     /locus_tag="RH222_001919"
     CDS             complement(10341..10730)
                     /gene="mutT"
                     /locus_tag="RH222_001919"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV3796949.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(10790..13495)
                     /gene="secA"
                     /locus_tag="RH222_001920"
     CDS             complement(10790..13495)
                     /gene="secA"
                     /locus_tag="RH222_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV3796950.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13557..14069)
                     /gene="secM"
                     /locus_tag="RH222_001921"
     CDS             complement(13557..14069)
                     /gene="secM"
                     /locus_tag="RH222_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV3796951.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14300..15217)
                     /gene="lpxC"
                     /locus_tag="RH222_001922"
     CDS             complement(14300..15217)
                     /gene="lpxC"
                     /locus_tag="RH222_001922"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV3796952.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15318..16469)
                     /gene="ftsZ"
                     /locus_tag="RH222_001923"
     CDS             complement(15318..16469)
                     /gene="ftsZ"
                     /locus_tag="RH222_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV3796953.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16530..17792)
                     /gene="ftsA"
                     /locus_tag="RH222_001924"
     CDS             complement(16530..17792)
                     /gene="ftsA"
                     /locus_tag="RH222_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV3796954.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17789..18619)
                     /gene="ftsQ"
                     /locus_tag="RH222_001925"
     CDS             complement(17789..18619)
                     /gene="ftsQ"
                     /locus_tag="RH222_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV3796955.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18621..19541)
                     /gene="ddlB"
                     /locus_tag="RH222_001926"
     CDS             complement(18621..19541)
                     /gene="ddlB"
                     /locus_tag="RH222_001926"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV3796956.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19534..21009)
                     /gene="murC"
                     /locus_tag="RH222_001927"
     CDS             complement(19534..21009)
                     /gene="murC"
                     /locus_tag="RH222_001927"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV3796957.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21063..22130)
                     /gene="murG"
                     /locus_tag="RH222_001928"
     CDS             complement(21063..22130)
                     /gene="murG"
                     /locus_tag="RH222_001928"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV3796958.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22127..23371)
                     /gene="ftsW"
                     /locus_tag="RH222_001929"
     CDS             complement(22127..23371)
                     /gene="ftsW"
                     /locus_tag="RH222_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV3796959.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23371..24687)
                     /gene="murD"
                     /locus_tag="RH222_001930"
     CDS             complement(23371..24687)
                     /gene="murD"
                     /locus_tag="RH222_001930"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV3796960.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALHPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24690..25772)
                     /gene="mraY"
                     /locus_tag="RH222_001931"
     CDS             complement(24690..25772)
                     /gene="mraY"
                     /locus_tag="RH222_001931"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV3796961.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25766..27124)
                     /gene="murF"
                     /locus_tag="RH222_001932"
     CDS             complement(25766..27124)
                     /gene="murF"
                     /locus_tag="RH222_001932"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV3796962.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27121..28608)
                     /gene="murE"
                     /locus_tag="RH222_001933"
     CDS             complement(27121..28608)
                     /gene="murE"
                     /locus_tag="RH222_001933"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV3796963.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28595..30361)
                     /gene="ftsI"
                     /locus_tag="RH222_001934"
     CDS             complement(28595..30361)
                     /gene="ftsI"
                     /locus_tag="RH222_001934"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV3796964.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30377..30742)
                     /gene="ftsL"
                     /locus_tag="RH222_001935"
     CDS             complement(30377..30742)
                     /gene="ftsL"
                     /locus_tag="RH222_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV3796965.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALSGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30739..31680)
                     /gene="rsmH"
                     /locus_tag="RH222_001936"
     CDS             complement(30739..31680)
                     /gene="rsmH"
                     /locus_tag="RH222_001936"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV3796966.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31682..32140)
                     /gene="mraZ"
                     /locus_tag="RH222_001937"
     CDS             complement(31682..32140)
                     /gene="mraZ"
                     /locus_tag="RH222_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV3796967.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32742..33746)
                     /gene="cra"
                     /locus_tag="RH222_001938"
     CDS             complement(32742..33746)
                     /gene="cra"
                     /locus_tag="RH222_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV3796968.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33828..33914)
                     /locus_tag="RH222_001939"
     CDS             complement(33828..33914)
                     /locus_tag="RH222_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV3796969.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33926..34417)
                     /gene="ilvN"
                     /locus_tag="RH222_001940"
     CDS             complement(33926..34417)
                     /gene="ilvN"
                     /locus_tag="RH222_001940"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV3796970.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34420..36144)
                     /gene="ilvI"
                     /locus_tag="RH222_001941"
     CDS             complement(34420..36144)
                     /gene="ilvI"
                     /locus_tag="RH222_001941"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV3796971.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36263..36307
                     /locus_tag="RH222_001942"
     CDS             36263..36307
                     /locus_tag="RH222_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796972.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36462..37406)
                     /gene="leuO"
                     /locus_tag="RH222_001943"
     CDS             complement(36462..37406)
                     /gene="leuO"
                     /locus_tag="RH222_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV3796973.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9060 a        10488 c         9181 g         8880 t
ORIGIN      
        1 gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca tgcataaagc
       61 gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat
      121 tcaaacttct ttgatgtaaa caaattaata caacaaacgg aattgcaaac ttacacacgc
      181 atcactgcgt agatcaaaaa aacaaccacc gcacgaggtt tcatgatgga aggtcaacag
      241 cacggcgagc agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt
      301 ggcgcgatag ggaccgggtt attcctgggt agcgcctccg taatacagtc cgcagggcca
      361 gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg
      421 ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac
      481 tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt
      541 gccatggctg agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatcccc
      601 acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt
      661 aaagtgtttg gcgagatgga gttctggttt gccattatca aagttatcgc ggtggtagcg
      721 atgatcatct tcggcggctg gctgctattc agtggcaacg gcggcccgca ggcgaccgtt
      781 agcaacctgt gggatcaggg tggtttcctg ccgcacggct tcaccgggct ggtgatgatg
      841 atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa
      901 gctgataacc cggagcaaag tataccgaaa gcaactaacc aggttatcta ccgcatcctg
      961 attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc
     1021 gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat
     1081 gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc
     1141 aacagccgta tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct
     1201 gtcgataaac gtggtgtacc agtaaatacc attctggtgt ctgcactggt aacggcgttg
     1261 tgcgtactga ttaactacct tgccccagag tccgctttcg gactgttaat ggcgctggtg
     1321 gtatctgcac tggtaatcaa ctgggcgatg attagcctgg cgcatatgaa attccgtcgc
     1381 gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac
     1441 tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg
     1501 gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt
     1561 aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccgtaca
     1621 gggtgagggc aataatcttt acaccaacgc cccgtaaatc gtcagtagtg caatcaccac
     1681 cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt
     1741 atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg
     1801 cgaagtatgg aaatcaccca gcgaagcaaa ccaggccggt aacgctttct caccatgacc
     1861 gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac
     1921 ggcatcaatg ccggactgta aacgatgatg cggcgtctga tatcgcgcca gccagtattg
     1981 ccatgcacgc aaaaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat
     2041 cagccagaac agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag
     2101 ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc
     2161 gccagccatc gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg
     2221 ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag
     2281 tagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc
     2341 catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa
     2401 gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa
     2461 aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcacacga
     2521 aaccgtgccc aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa
     2581 tggcccgtca tgtttttagc gatatccgga tagcaatcaa tcagtgcccg cgtaaccgcc
     2641 gcaagctgtt gatactgcgc atcggtatac gccagcgtat cggtgccttc aagctcaatc
     2701 ccaatagaaa aatcattgca gcgttcgcgc ccctgatact gagagactcc cgcatgccat
     2761 gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga
     2821 gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt
     2881 ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg
     2941 tgcaccacca gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga
     3001 acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt
     3061 ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga
     3121 gttttaacat gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac
     3181 gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa
     3241 cagtcgatgc caacaatgat attacggcaa aacttttacc ggaaaattct cgctctcatg
     3301 ccacggtgat cacccgcgag aatggcgtct tttgcggcaa acgctgggtt gaagaggtgt
     3361 ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca
     3421 tcaatgccaa tcaatccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg
     3481 aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact
     3541 atgtcgaatt gctggaaggc accaacacgc agttgttgga tacgcgcaaa accttacccg
     3601 gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg
     3661 ggctttctga tgccttcctg atcaaagaaa accatattat tgcctccggc tcagtgcgcc
     3721 aggcggtcga aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga
     3781 atctggaaga acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact
     3841 tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag
     3901 tgtctggcaa cgtcactgac aaaacactgc gtgaatttgc cgaaacgggc gtggacttta
     3961 tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct
     4021 aatagttgac agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc
     4081 ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaatagaaaa cctcggaatc
     4141 tgctttcccg tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc aaagtagcgc
     4201 caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg
     4261 atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac
     4321 tacctgcgca aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc
     4381 gtagagttgt gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc
     4441 attccctcgc ctaccaccac ccactatgtt tcagccatga gtgtggcaaa aggcgtggtg
     4501 tcgctgaccg ggcaagaaag tctcaatgga ctaagcgtcg tcatgacacc gggttgggat
     4561 aacgcaaacg gcgtcaccgg ctggacgcgc aactgcaata ttcaaagtga cagcgcattg
     4621 cagcaagcct gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat
     4681 attccacagc tcacggccct gtgtctgcgt tatcagggag tcttgctgga tgccagcgaa
     4741 gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg agttgctgga cgcattgcat
     4801 ttcgctacca ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggtcac
     4861 gccagtcgca cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca
     4921 gagttgctag ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga tattcatatc
     4981 gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta
     5041 ccggatgttt caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac
     5101 ctggatattg cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga
     5161 aacgccgtct catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta
     5221 aggttgttac agcaggtggg tcaggcactg gatgtcaaca cgcttggaat gcagccgtta
     5281 caactggcgg actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc
     5341 cctaccggca gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaataccgct
     5401 gacattaata tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag
     5461 acgcaaatcc atccgcgtgc cgggctcacc tttcagggcg ttttgcgtgc gttattgcgc
     5521 caggatcctg acgtcatcat gatcggagag atccgcgatg gcgaaacagc agagatcgct
     5581 attaaagcgg cgcaaactgg tcacctggtg ttgtctaccc tacacactaa ttccacctgc
     5641 gaaacgctgg tacgtttaca gcaaatggga gtcgcccgct ggatgctctc atcagcgctt
     5701 acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg ccagcagcaa
     5761 ggggagccca tccatattcc agtcaatgta tggccgtcgc cgctgcccca ctggcaggca
     5821 cccggttgtg tacattgcta ccacggtttt tatggtcgta cggccttatt tgaagttctg
     5881 cccataacgc cggtcattcg tcagcttatt tccgctaata ccgacgttga atcgctggaa
     5941 acgcacgcac gacaggcggg tatgtgtacg ctttttgaaa acggctgcct ggccgtggag
     6001 caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca tggcgagtaa
     6061 gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg
     6121 ggcagagagc cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccacttag
     6181 cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat
     6241 tcatcaactg gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggcactgct
     6301 ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg cgcacgatct
     6361 cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggtat ttccgccact
     6421 ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga
     6481 actggcgcgt cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg
     6541 ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaattc tgcattttgt
     6601 tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg cactaacgca
     6661 ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg tgttgttcgg
     6721 ctttctgctg gcgatagcca ataagttgct gatgcgccgc cctacctggc ttatcgcgcg
     6781 gcagaaattg ttgttacgaa tcccgattat gggttcactg atgaggggac aaaaactcac
     6841 gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt tacagggcgt
     6901 agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca
     6961 gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg gtgagtttag
     7021 cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctctctgg atctcatgtt
     7081 agacaacctc gcccatcatc atcgggaaaa cacaatggcg ctggcggata acctcgcagc
     7141 cttactggaa ccggcgttgc tgatcataac gggaggaatt attggtacgc tggtggtggc
     7201 aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat aacgctggcg
     7261 tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt ttcttgttcc
     7321 tgcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc cccaacgtat
     7381 gtacaagctg aacgcaggcc gcccaaaata tctcgcgcgg tattttcaac cgggcctcgc
     7441 agcggcagct taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga
     7501 cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc gccgttctcc
     7561 tcaacgatgc gaccgccgct ctcttcgtag cccgccagca tgccgccaag catgacgaaa
     7621 tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtgcagcc accatcgctg
     7681 acgatcattc cgcccagacc gtgcgcagca tcggcacatt cgattaccgc agaaagttgc
     7741 ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact
     7801 ttaacgatat cggcacctga gaggataagc tcctcacaca tttcaccagt cactacgtta
     7861 ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag
     7921 tgttcggaat aaccattcgc cacgtcaata caaacgaagt ttaatgccgg gttcaggtcg
     7981 agaatctgtt tagttttttc gaaatccgca tcagacgtac cggtagaaac catcacatgt
     8041 ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac agaatagtgt
     8101 ttatgcacag cagtcaaaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct
     8161 acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc tgaatgtttg
     8221 aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg
     8281 cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc
     8341 ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca tacgcactaa
     8401 tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc
     8461 tggtaaatag tgagcgaacg tttcatcaat ttcgctttcg tgtactcatt tttaacgttt
     8521 tgataatcat gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg
     8581 aggcattggc agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt
     8641 cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc
     8701 cattgctgat cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc
     8761 cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca
     8821 tccgctgatt cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact
     8881 gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt
     8941 ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg atgtaactcg
     9001 cgagcatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc ttgccgtggc
     9061 agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct
     9121 gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag
     9181 acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag
     9241 tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat
     9301 ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt ccgcactgag
     9361 ctgttgaaag aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcctggc
     9421 gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta
     9481 atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc tctggtgcgt
     9541 cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt gcacatttgg
     9601 ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac
     9661 ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt
     9721 aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc
     9781 ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa gagccgtttt
     9841 gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg tctggatttc
     9901 gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc
     9961 tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt
    10021 tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc
    10081 gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt gtctgatgcc
    10141 gcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc aaatgtcgga
    10201 tgcggcgtga acgccttatc cgacctacaa aaacaaattt atcaatgagt tagaaatccc
    10261 tgtaggcctg ataagacgcg ccagcgtcgc atcaggcatc ttgtgcgaat gtcggatgcg
    10321 gcgaaaacgc cttatctgac ctacagacgt ttaagcttcg caattaccgg ttcattggct
    10381 ggcggaaaat catcggcatt aagatcgacc agcgccatcc actcaccggg ttgcccttct
    10441 ttaccccacg gcttcccttc ccagcgttcg accagccaga accacagtgt tatatgcctg
    10501 tccgggaatt catattccag tttttcaaat agcgaaaaat gttgtggggt aatcccgact
    10561 tcttcctgaa gttcacgcac caccgcctgt tccggcgttt cacccatttc aattttaccg
    10621 ccgggaaact ccagtttatt cgccatgtgc gcatctgctg cgcgacgcgt tataaagatt
    10681 tcattgttct cgttgcgaat aatacctacc gcaatttgca gctttttcat tgtcttaaac
    10741 ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac tgttagcttt tattgcaggc
    10801 gaccatggca ctgcttgtat tttttaccag aaccgcacgg gcaaggatcg ttacgtccta
    10861 ctttgcgttc accggtttgc gccgccagtg cagctgcggc tgcagagtcg tcatcctgat
    10921 ggctaagctg ctgcatttgc gctaaacgct cggcttccat acgacgctgt tgttccagct
    10981 cctcaacttc ttcaggcata cgtacctgaa ctttgctcag cgtactgata acttcatatt
    11041 tcaacgactc cagcatcgct gcaaacatgg agaacgattc acgtttgtat tcctgcttcg
    11101 gatctttctg tgcatagcct cgcaggtgga taccctgacg cagatagtcc atcgctgcca
    11161 ggtgctcttt ccacagggag tcaagcgttt gcagcatgac gcctttctcg aagtgacgca
    11221 tcatctctgc accaaccact tcttctttac gctgatacac ttcgatggac tgcgccagaa
    11281 tgcgctcacg cagcgtctct tcatgcagtt cgggttcttt atccagccac tcagcaattg
    11341 gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag ccccggaata tcccacattt
    11401 cttccagcga ctgcggcgga atgtaggcat caatggtggc tttgaacaca tcttcacgaa
    11461 tgctgttgat ggtttcgctc acatcgctaa catccagcag ttcgttacgc tgggagtaaa
    11521 tggcgcgacg ctgatcgtta gccacgtcat catattccag cagttgctta cgaatgtcga
    11581 agttacgact ttcaacttta cgctgggcgt tggcaatcgc tttggtcacc cacgggtgtt
    11641 caatggcttc gcctggcttc atacccagtt tacgcatcat gccggatact cggtcggaag
    11701 caaaaatacg catcagcgca tcttccatcg acaggtagaa acgggaagaa ccagcatccc
    11761 cctgacgacc agaacgaccg cgcaactggt tatcgatacg acgggattcg tgacgctcgg
    11821 taccgatgat atgcaggcca cctgctgcca gtaccgcatc gtgacgtacc tgccagtcgg
    11881 ctttaatttt ttcaatttgc tctgcggtcg gattttccag cgcggcaact tctgcctgcc
    11941 agctaccacc gagcacaata tctgtaccac gacccgccat gttggtcgcg atagtcaccg
    12001 cagccggata acctgcctga gcaacaatcg ccgcttcgtt ggcgtggaat ttggcgttca
    12061 ggacattgtg cttaataccg gctttggtca gttcgtttga caccagctcc gatttttcga
    12121 tggagatagt acccaccagc accggctggc ctttcgcagt acgttctttg atatcttcaa
    12181 tgatcgcctg aattttttcc gcttcagtca tgtagaccag gtccggcaaa tctttacgaa
    12241 tcattggacg gttggtcgga acaacgacgg tatccagctt gtagatggag ctaaattcga
    12301 aagcttcggt atcagcagta ccggtcatcc ccgccagttt ttcatacaga cggaagtagt
    12361 tctggaaggt gatcgaagcc agcgtttggt tttcgttctg gatctgcaca ccttctttcg
    12421 cttccacagc ctggtgcaga ccatcggacc agcgacggcc ctgcatggta cgaccggtgt
    12481 gttcgtcaac gatgataact tcaccatctt taacgatgta gtcgacgtcg cgggtaaaca
    12541 gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag catgatgttg gccggagagt
    12601 acagagactc cccttcatcc atgatgccct ctttcaccag cagttcttca atcagcacca
    12661 gaccacgttc ggtcaggttc acctggcgag atttttcgtc caccgagaag tggccttcgc
    12721 cctggaaggt ttcggagtct tctttttcct gacggatcag gtgcggaata attttattca
    12781 cgcgtttata catttccgag ctgtcttctg ccgggccgga aatgatcagc ggtgtacgcg
    12841 cttcatcgat caggatggag tccacttcgt ccaccagcgc atagtgcagt ttacgctgta
    12901 cacgttcttc agggctgaac gccatattgt cgcgcaggta gtcaaagccg tattcgttgt
    12961 tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt tgccggtgct ggcatgcccg
    13021 gcaggttgat accgacagtc aggccaagga attcaaacag cggacggttg ttttcggcgt
    13081 cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac gcctttaccg gttagtgcgt
    13141 tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc accggtacgc atttcggcga
    13201 tgcagcgttc gttaagaacc ataccgccga gtaactgaac gtcgaagtga cgcataccaa
    13261 agacgcgctt acttgcctca cgtaccacgg cgaaagcttc cgggatcaga ttttccagca
    13321 cttcgccttt ttccagacgt gcacgaaact ctgcggtttt ccctttcagt tcttcgtcgg
    13381 agagtttttc catctccggt tccatggcat tgatgatgtt gaccactttg cgcatccggc
    13441 gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa caatttgatt agcataataa
    13501 aatctcaaac gccccgcgtt gcggagttaa taaaatgaag taaaggttta ttgttgttag
    13561 gtgaggcgtt gagggccagc acggatgcct tgcgcctggc ttatccagac gggcgtgctg
    13621 aattttgctt gtggggtaaa atgcgcataa tcaatgcgat aacccttttc agacggcgtg
    13681 ccttcctggg tcagcagcgc gctgagcgta tccagtaatg caagatgttg cgcctgaaga
    13741 ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca ttgcgaaaga aagatgacgg
    13801 attaccgtgc gaatggcatg ttgatgccag taatcaacgg aatagttcga attcgggcgg
    13861 cgtgtgttcg cttccagcaa ggccaattga ccaaagttaa ctttggctga aggctcgtgg
    13921 ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg cgttgctgag cgcaggcaaa
    13981 cctaaactcg ccgcaaccat ccctaataag agatgcggcc agaagtagcg tttaccaaac
    14041 tgtcgccagc gcgtcagtat tccactcacg ttattgccat cccgatcgta ttcaaacgta
    14101 tttagcgcac aaatcttatc attaaagcct gcagtccaca gcacgaatga agataagttg
    14161 tgcggaaaag tgcttaagaa aacagcgttc gcaccgtttt ttatagctct tataaaattc
    14221 aaaaaaaaac gactggttca cctggccgga gagagtgcca gatttgccag tcgaatttta
    14281 tacgacagta taaatgtcgt tatgccagta cagctgaagg cgctttgaag gccaacggca
    14341 gttctgcgtc gtcctggaag gtcacatatt cccaggcttc ctgtttcgcc aggacagcct
    14401 gcagcagttt gttattcagt gcatgaccgg atttataagc ggtaaatgca ccaataatat
    14461 tgtgaccaca catgaacaag tcaccgatcg catcgagcat tttgtgacgc acaaattcgt
    14521 cttcaaaacg caggccgtct tcgttcagta cgcgataatc gtcaacaacg atggcacaat
    14581 cgaagctgcc gcccaggcac aaaccacggg actgcagata ttcgatatca cgcatgaaac
    14641 cgaacgtacg cgcacggctg atctggcgca taaacgcatc agcggagaag ttcatcgcat
    14701 agcgctggtt gctggaatca atagccggat ggttaaaatc gatggtgaaa tccagcgaaa
    14761 aaccattgta cggcttaaat tcagcccact tatcgccatc ttcgacacga acagtctctt
    14821 tgatgcgaac aaattttttg gcgcagttca actcgtcgat accggcgtca agcagcaggt
    14881 atacaaacgg agcggcgctg ccgtccatga tcgggatttc cggcgcgtta acttcgataa
    14941 caatgttatc gatgcccagg cccgcgagag cagcattgag gtgctctacg gttgaaatcc
    15001 gtacatcatg ctcgttgacc agacacgtac agagcatggt atcacgcaca gatttggcat
    15061 cggccgggaa atctaccggt ggattcaagt cggtgcgacg atagatgacc ccggtgttgg
    15121 ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt atgtaaaccg acacccgtcg
    15181 cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt attatctcgc caaattacct
    15241 atccaaccga agtgtactat acattcggcg ggccagttta gcacaaagag cctcgaaacc
    15301 caaattccag tcaattctta atcagcttgc ttacgcagga atgctgggat atccagataa
    15361 tccggctctt tcgcagtttg cggcgcattg tcattcacga ctttagcaac tggcttctgc
    15421 tcctgggtca gcggagccat cccatgctgc tggtagcgat ccatcactgg ctgctgaacc
    15481 tgcttattgg tcaccagagt gatttcagga cgtttgtcca tgccgatacc tgtcgcaaca
    15541 acagttacgc gcagctcgtc attcatatcc gggtcaagag aagtaccgat aaccacagtc
    15601 gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg tttcgaactc atccagacgc
    15661 aggtcgaagc ccgctgtgat gttaaccagc acgccgcgtg cgccagatag gtcgatatct
    15721 tccagcagcg gagaagaaat agccatttca gcagcttctt ccgcacggtc ttcaccgctc
    15781 gccacgccag aacccatcat tgcgtagccc atttcagaca ttacggtgcg tacgtctgca
    15841 aagtccacgt tcatcaaacc cggacgagta atcagttcgg cgatgccctg aacagcgcct
    15901 ttcagtacat cgttcgctgc gccaaacgca tccagcaggg agataccgcg gcccagaact
    15961 ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca catgcttgga cagttcagtg
    16021 atcccctgct ccgcgaatgc catacgcttc ttgccttcaa agttgaaagg cttagtgacg
    16081 acagcaacgg tcaggatacc caaatctttt gccacttcag cgacgactgg tgctgcacct
    16141 gtaccggtac caccacccat acccgcagca ataaagacca tgtctgcacc ttccagcgcc
    16201 gcacgcagtg catcgcgatc ctcatcagcc gcattgcggc caacttctgg attagcgcca
    16261 gcgcccagtc ctttggtgat accgctaccg atttgaatcg tctgtccaac cgctgtttta
    16321 cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa caccttcaat acgctcgcgc
    16381 accatgtgtt caacagcatt accgccgccg ccgccgacgc cgatgacttt aatcaccgcg
    16441 tcattggtaa gttccattgg ttcaaacata gtttctctcc gatttgtgcc tgtcgcctga
    16501 ggccgtaatc atcgtcggcc tcataaaaat taaaactctt ttcgcagcca actattgagt
    16561 cgcttgatcc acgagccaac tgatgctgta acacgttttt ctacttcagc ttcaccgtta
    16621 agatgtgact ctttcccata gtgaagcaat cccaccgccg tcgaataata cggctcctga
    16681 gcataatccg ttaaaccggt aatgttcagc ggcgcgccga tacgcacttg cgtatgaaac
    16741 acgcgctgag cacaggctgc aagaccttcg atctgcgctg cgccaccggt taatacaatg
    16801 cctgccgcca ggtgatgttt aaccccttgt tggcgaagct tttcctgcaa ctgcaatatc
    16861 tcttcgttga ccaggttgag cagctcggta tagcgcggct cgatcacctc tgccagggtc
    16921 tgacgttgca gactccgcgg cggacgacca cctacgctcg gcacttccac gctctcatct
    16981 tttccaacga tggaacccag cgcacaaccg tggcgaactt taatcgcttc ggcgtcgctt
    17041 ggcggcgtgc caaaggcgta agcgatatca ctggtcacga cattgccagc ataaggaatt
    17101 accttagtgt ggcgcaatgc cccaccggta taaacggcga tatccattgt accaccaccg
    17161 atatcgacga cgcagacacc cagttcacgt tcatcttccg tcaataccga ataacttgat
    17221 gccagtccgg caaatatcag ttggtcaact ttcagcccac aacgttcaac cgctttgacg
    17281 atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca cttttgcctg catccgcacg
    17341 cccgaaagtc ctaccggatt cttgatccct tcctgatagt caatcgcata ctcttgcggg
    17401 atcacatgca gcacacgatg ctcatcgcgc acacgcaccg atttcgcggt atggacgacg
    17461 ttttccacat cttcttgcgt cacttcttct tcagaaatag gcaccatacc aatttcattc
    17521 tggcagctga tgtgcttacc agaaagcgcc agatataccg aagagatctg acaatctgcc
    17581 atcaattctg cctggtcaat ggcgcgttgt acgcacttga ccacggattc gaggtcgttc
    17641 accccgcctt tatccatacc acgcgacggg cagctgccca cgccaatgat attgaccata
    17701 ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg cggtaccaat ctccagtcct
    17761 actaccagtt ttctgtccgt cgccttgatc attgttgttc tgcctgtgcc tgattttgtt
    17821 gctgagtaga ttcctctggc ggcaagggcg cccagcctac tgccgctcca gagtcataac
    17881 gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc ctgctgctgt aaaaccggat
    17941 aaagttctac aaagcgagcc aaacgtttca tcgtatcgcc ccggccaaga ttgagcttaa
    18001 tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt catcgccgct tccttcagag
    18061 taaatctgtc ctttgccagc atctgcccca tttcgcgata gccctgcaac acttcattgg
    18121 cgctgccttc cgggccatac agcattggaa gcacctgctt gctggtgcgt tctggcggca
    18181 cgctgaaggt atttccttcc gcgtctacca tatgttgatc attccaccgc gcaatcggca
    18241 catattcaac cagatgaatc ttcaattcat caggccactg ctttctgacg ctcacctgct
    18301 taatccacgg caggcgttgt tctatttgcg tctggatgat gttgacatcc tgggtcataa
    18361 aggtacccgg ctcacccaat gccaggatcg actgccggat atcgtcatta cgtgtgtaat
    18421 ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg ttgcgcatct tccatccagc
    18481 ccaacacgac ccagccgctc accaacactg tcgttaaaac ggtcagcagg aaaaggatcc
    18541 ccgccagacg cgttccatta ttgcggcgag aagaaacctc ttcttcgctg tttcgcgtgt
    18601 tcagagcagc ctgcgacata ttagtccgcc agttccagaa ttcgtactac caactgcgag
    18661 aagctcatac ctgcctgacg tgccgccatc ggcaccaggc tgtggctggt catacccggt
    18721 gaggtattgg cttccagcag ataaaactgt ccatcgctgt ccagcataac gtcaatacgt
    18781 ccccatcctt tgcaacctaa cgtcgtccat gctttcagca ctaatgcctg caaattggcc
    18841 tcttgtgacg cttccagacc tgcggggcag aaatactgtg tctcatcaga gagatacttc
    18901 gcctcataat catagaaggt tccggacggt tgaatacgta ttgacggtaa aatttcttca
    18961 ccgagtatcg caaccgtgaa ctccggccca cttagccatt tttcaatcaa tacttcttca
    19021 tcgtgctgaa atgccaatct taatgcatct tgtagagcat tttctgctac tacttttgac
    19081 attcccacac tggaaccttc gcggctcggc ttaacgataa ccggcaaacc cagagcagaa
    19141 atttctgcta actgcttatc gctcaggcct ttttcaaact ctgcgcgggt taacgctacc
    19201 cacggcgcga ccggtaaacc ggcaccttgc catagaagtt tgctgcgtag tttatccatt
    19261 gaaagcgcag atgccatcac tccgcttccg gtataaggca agcccatcag ctcgagcatc
    19321 ccctgcagcg taccatcttc accgccgcga ccgtgtagcg cgataaacac tttctgaaag
    19381 cccatcgact tcagttgcgt cacgtcgact tctttcgggt cgacaggata cgcgtcaata
    19441 ccgccttcac gcagtccggc taacaccgct gcgccagaat tcagagaaac ttcccgctca
    19501 gcggaggtcc cacccaacag gaccgcgatt ttatcagtca tgttgttctt cctccggagt
    19561 ttgcggcttc agtttgattt cagctaaaga acgagcaatt tttccaatat taccagcccc
    19621 ctgaacgaga atcaggtcgt taccggttaa taccggtgcc agcatctcgg ctacccgcgc
    19681 cggatccggc accagaatgg gatcaatttt cccacgtcca cgaattgtgc gacacagcga
    19741 acggctgtcc gctcccggaa ttggcgcttc gccagccgga tacacttcca gcatcaacag
    19801 ggtatcaacc tgcgtcagca cattggcgaa atcatcatac aggtcgcgcg tacgggtaaa
    19861 acggtgcggc tgaaacagca ttaccaggtt tttatccggc cagcctgcgc gcgccgcttt
    19921 aatggtggcg tccacttcag tcgggtggtg gccgtagtca tcgaccagca ttgccgtacc
    19981 gcttttacca ttcactggct ccagcgggaa ttcaccgagg aaatcaaaac ggcgaccagt
    20041 cccctggaag ctttcaagcg cccgcaaaat agcctcgtcg tcaatgccct cttccgtagc
    20101 aaccgcaacc gcagctgcgg cgttcagcgc gttatgacga cctggcgcat tcagggtgac
    20161 gcgcatcggc tctttgtcct ggcgcagcag cgtaaagtgc ccctgcgggc caatctgctg
    20221 ataatcttct acacgcacgt cggcatcttc gctgaagccg taagtcgtgg tctgacgccc
    20281 cactcgcggt aacaattcgc ggatcaccgg atcatcaaca cacatcaccg cacgaccgta
    20341 aaacggcagg ttgtgcagaa aattaataaa agtctgtttt aaattctcaa agtcgccctg
    20401 gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc gccaccatcg gttgcagatg
    20461 caggaacgat gcatcactct catctgcttc ggcaatcagg taccgaccat gccccaaacg
    20521 cgcatgaacc cccgccgctt ttaccagccc gccgttaacg aaggttgggt cgagccccgc
    20581 ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt ttgccgtgcg ttcctgcaat
    20641 ggcgatgcca tgacgaaaac gcattaactc agccagcatt tcggcacgac ggatcaccgg
    20701 aatacgcgct tcatgagctg cgacaatttc cgggttgtcg gcagaaatcg cgctggaaac
    20761 aacgaccacg ctggcatcac gtacgttttc cgggcgatgg ttgaaataaa tcgtcgctcc
    20821 cagattcatt aactgctgcg tgaccggatt tggcgctaaa tcggaaccac tgatctgata
    20881 accttcattg gccagaactt cggcaatacc gcccataccg gcaccaccga tgccgacaaa
    20941 atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa cgcagttttg ccaattgttg
    21001 tgtattcatt ctttacgcca ttaacttctt aaattcatac gatgcaaaag gcatcgctac
    21061 aattacgccc gggcagcccg gctcacttca tttgccactc gctcggtggc atccggaatg
    21121 gatgcagcgc gggcgcgttc tgccatggtt aataaggttt ctcgcgacca cccggccagg
    21181 gtgttggcga cagcatccac gctaagctgt ggctgttcga taattttggc tgcgcccgct
    21241 ttttccagcg gtagcgcatt ccagtattgc tggcggtctt tatgttgaaa cggcacaaac
    21301 aacgccggta gtcctgccgc ggcgatttca ctcaccgtta acgcaccgga gcggcaaacg
    21361 acgacatccg cccacgcata cgccgccgcc atatcatcaa taaattccgt cactttatgc
    21421 tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt gttgcgaacc tttgccgctc
    21481 tgatgccaga tagtgactga atcacccagt ttcgcagcaa cctgcggcat tgtctggtta
    21541 agaatgcgtg cgccctgaga cccccccact accagcacac gaaccggacc ttcacgtcca
    21601 gccaaacgtt gctgcggcaa cggcagcgcc aacacatcgg tacgcaccgg gttacccact
    21661 acttccgcat tagggaaagc acctggaaac gcctgcatca ctttggtggc aatcttcgcc
    21721 agccatttat tggttaagcc cgcaataccg ttttgttcat gaagtacaac cggaatgcct
    21781 aacgaccacg cggccagacc acctggacct gacacatagc cgcccatacc gagcaccacg
    21841 tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc aggcattgaa gatacgcagc
    21901 ggcgcagcta tcagtgcttt tatacctttt ccacgcagac cagagatacg aatgaaatca
    21961 atttcgatgc catgttttgg cactaagtcc gcttccatac ggtcagcagt ccccagccag
    22021 cgaacttgcc aaccctgagc cattagatgg tgcgcaaccg ccagtcccgg gaatacatgt
    22081 ccaccggttc cgcctgccat caccattaat cgctttcctt gaccactcat cgtgaacctc
    22141 gtacaaacgc ctgcgctttc tccagacgcg tttcataatc aatacgcaac agcatcatga
    22201 tggctgtcga cataatcagt aagctcgaac caccgtaact gatcagcggc aatgtcagac
    22261 ctttggtcgg taacatcccc gccgccgcgc ctacgttaac cagcgcctgg aagctaaacc
    22321 agatgccaat ggaacaggcg agaaaaccgg aaaaacggtg gtcaatttct aacgctttgc
    22381 ggccaatcga catcgcacga aaagcgacga agaataccat taaaagtgcc agcaccacac
    22441 cgacataccc cagttcttcg ccgataatgg cgaaaataaa gtcagtgtgc gcttccggca
    22501 gatactccag tttttgtacc gagttaccta aaccttgccc ccaaagttcg ccgcgaccaa
    22561 acgccatcag cgattgcgtt aactgatagc cgctgccaaa gggatcttcc cacgggttcc
    22621 agaatgcggt aacacggcgg atacggtacg gttcggcgag tatcagcaac acaaccgctg
    22681 aaatgcccat accgataatg gcaatgaact gccacaattt cgctcccgcc aggaacaaca
    22741 tcgccagcgt agtcacaaac aacaccacca ccgtaccaag gtctggctgt gccagcagta
    22801 acactgccaa caccagaatc acgcccatcg gtttcaggaa gccgcgcagg ttattacgta
    22861 cttcgtcgcc tttacgcacc agatagttgg cgatatagca aaacagcgac agttttgtca
    22921 gctccgcagg ctggatacgc agcaaaccga gatcgatcca acgcgatgcc cctttaaccg
    22981 agctacccac taccagaacg atcatcagca ggatgataga tccgagcagc atcgtggcac
    23041 tgtagcgttg ccagaactcc atcggcagac gcagcgtaat gatcgccaga ataaacgcca
    23101 aaatcagata gacaccatca cgcttcgcga agaagaacgg atcgttggtt aagcgttgcc
    23161 ctatgggcat tgacgccgag gtcaccatga taaagccaat cgccgcgagg ccgaaggtca
    23221 gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt atctttttcc cgcgagccca
    23281 tcacccagcc ctttagcgcc gtggagatcc agaccaggat actgaatcct ggcaggcgcg
    23341 gcattttcag gcgagggaga gataaacgca tcaacctaac tccttcgcca gacgggcaaa
    23401 ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg ctggcacagg ccggggagag
    23461 tagaaccata tcgcccggct gaacacgcgg agccagcaag cgcatcgcct gttccatagt
    23521 ttcggtttgt tctgccactt ccgggtgtag cgccgccagc tgcgcgccgt cacgaccgaa
    23581 acaatacaga cgtacgttat cgccattcag gtaacgcgcc agtgggctaa agtccgccga
    23641 tttaccatcg ccgcccagca acaaatgcag cgtgccgtct acgtgcaggc cattcagcgc
    23701 cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta atccaacgta cgccgttatg
    23761 ctccagcaca acttcaaagc gatgcggcag accagtgaat gtggttaacg ctttcaggct
    23821 gctggcacgc ggtaaccctg cggcatctgc cagtgccagc gccgccagcg cattggtgta
    23881 gttatgctgc ccggaaagtt tcatctcttt cacgttcagc actttctcgc ctttaacccg
    23941 cagccaggtt tcgccctgct gatgattcag gtgatagtca cccatgttga cgccaaagct
    24001 gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag gcatcatcgg cattaaccac
    24061 gcaaactttt gcgttttcgt aaatgcgcag ttttgctgca cgatactgtt gtaaaccaaa
    24121 cggatagcga tccatatgat cttcagtcac gttcagaatg gtcgctgcta ccgcctgtaa
    24181 gctagaggtg gtttccagct ggaagctcga cagttccagc acgtacagtt cgcactcatc
    24241 atccagcagc atcaacgcag gcaggccaat attgccaccc actccaacgt taacccccgc
    24301 cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt ttgccgttag aaccggtaat
    24361 cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg atatcgccaa cgatttcgat
    24421 tccggcatca gcggcagcgc ttaaggatgg atgcgccagt gcaataccgg gactggcgac
    24481 aatcagatct gccgccatca gccattcatc attcagactg cccgtgtggc gttctacggc
    24541 ttcgggtaat ttatccaggc caggcggtgt catacgcgta tccataacgc gcggcgtcac
    24601 accgcgagcg aggaaaaagt ccacgcagga aagcccggtg aggcccaggc cgataatgac
    24661 aacattttta ccctggtaat cagccatgat taacgtacct tcagcgttgc cagaccaatc
    24721 aggaccagca tcagcgaaat aatccagaaa cgcacaatga cgcgcggttc cggccagcct
    24781 ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa tacgttgtcc gcgcagttta
    24841 aaggagccga cctgcaggat gacagaaagc gtttctacca cgaacacgcc ccccataatc
    24901 accagcagga attcctgacg tagcagtacg gcgataatgc ctaacgcacc acctaacgcc
    24961 agcgacccga catcgcccat aaagacctgc gccggatagg tgttaaacca caggaagccc
    25021 agtcctgccc cgactatcgc ggtacagaca ataaccagtt ccccggcgtg tcgcagatac
    25081 ggtatgtgta aatagctggc aaagttcatg ttgccggtcg cccacgccac cagcgcaaaa
    25141 ccaccggcga caaatacggt cggcataatt gccagaccgt caagaccatc ggtcaggttt
    25201 accgcgttgc cagtacccac aatgacgaag taagccagca gaatgtagaa cagccccagt
    25261 tgcggcatca catctttaaa gaatgggacc accagctgcg ttgcgggcgt gtctttgccg
    25321 gcaaggtaca gggcgaaggc gacacccagc gcaatgaccg acatccagaa atacttccaa
    25381 cgagcgatca accctttggt gtctttacgc accactttgc gataatcatc aacaaagcca
    25441 ataacaccgt aacctaccag caccaccaac acgcaccaga cgtacggatt ggacgggtaa
    25501 gcccacagca gtacggagat cacaatcgcc gtcaggatca taatcccgcc catggtcggc
    25561 gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac gcaccacctg accaaaggaa
    25621 agtttttgca aatgagcaat catacgcggg cccatccaca atgagatgaa cagcgcggtc
    25681 agcaggctga cgatggcgcg aaacgtcaga taggaaaaga cgttaaagcc ggaataatat
    25741 ttgaccaaat gttcggccag ccaaactaac atgtcccatt ctcctgtaaa gcgcgtacta
    25801 cctcttccat ggcggcacta cgtgaaccct taactaaaat cgtaattacc tgttgctcag
    25861 caatcagtga tttaagacgc gtaattaacg cagttttatc agcaaaatgt tcgccaacgc
    25921 cgctggcggt gctgatagca tggctttgtt tacccacgct taacacgcgg tcaataccag
    25981 cagcttttgc cgcctcgccc acctgtacat ggcaggcttc gctttcagcg cccagttccg
    26041 ccatatcgcc caccaccagc acgcggtagc ccggcatttc agccagtacc tggactgctg
    26101 cagtcattga accgacattg gcgttgtagg agtcgtcgag cagcaactgg ttttctgcca
    26161 gttggatggg gaacagacgg cctggaacag ctttcagatt tgccagcccc gctttgatag
    26221 catcaagcgt tgcgcccacg gacatggaga gcgcagcggc tgccagcgca ttcgcaatat
    26281 tgtgacgccc cggcaacggc agcagaacat cgacgctacc ggttggggtt tgtagggtaa
    26341 attccgtacc gtgcgaggtc acatggatat tggtggcggt gaaatcgctg ttggcggcat
    26401 tgggtgagaa acgccacact ttgcgtgagc caattacgct ctgccagttc agccagtcgt
    26461 tgttgtcggc gttcataatg gcgataccgt tttccggcag gccgctaaag atttcacctt
    26521 tcgctttcgc gacacccgca agcgagccaa aaccttccag atgcgccgct gccaggttgt
    26581 tgaccagcgc agcttccggg cgagtcagac tcacagtcca ggctatttcg ccctgatggt
    26641 tcgcgccaag ttcaataact gcgtaatcgt attccggcgt taagcgcaac agcgtcatcg
    26701 gtacaccgat gtcgttgttg agattgcctg ccgtataaag cgtgttgccg cactggctta
    26761 aaatcgccgc cgtcatctct ttaacggagg ttttgccgga ggaccccgtc agagcaacca
    26821 cgcgcgccgg aacttgctgg cgaacccatg cagccagttc accaaacgcc agacgcgtat
    26881 ccttgacgat taactgcggc aggtcgatgt ccagcggacg gctaaccagt agtgcgcctg
    26941 cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc aaaacgttcg cctttcaggg
    27001 caacaaacag gcagcccggc gtcagttttc gggtatcagt ggttacagca tcaagggtga
    27061 tatctgcacc ttgcagttca ccgttgagaa tgtcggtaag ttggctaagg gttacgctaa
    27121 tcatgcaatc acccccagca gacgcgccac cgtgacgcga tcggagtagt ccagacgctg
    27181 attgccaaca atctggtaat cttcatggcc tttgcccgcg accagtacca catcattctc
    27241 tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg ccttccatca ctttggcatg
    27301 tccggcatct aacattcccg ccagaatatc gttgatgatg gcacgcggtt cttcggtacg
    27361 cgggttatcg tccgtcacca ccgccacgtc agcaaactct tcggcaattg cgcccatcag
    27421 tggacgctta cctttatcgc gatcgccacc acagccaaag acgcaccaca gcttgcccgc
    27481 acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca tccggcgtat gcgcgtaatc
    27541 caccaccacc gtcggtttgc ctggcgcagt gaacacttcc atacgtccgc aaaccggttg
    27601 cagacgcgcg gcggttttta acagatcagc cagcgggtag ccaagtgcca gcagtgtcgc
    27661 cagcgcgagc agcaggttgc tgacgttaaa agcgcccatc agatggcttt caatttcgcc
    27721 atcgccccaa cttgaactaa agcgaatggt cgcgccgctg tcgtgatagt tcacttcggt
    27781 cgctttcaac cagcgtccgt gacagttcgg attgatatga tcttccattg ataccgcaac
    27841 cgcgtccggc agttttgcca gccagcggcg gcccacttca tcgtcggcgt taacaatcgc
    27901 ctgaccacaa tgatgttcag aataaagcag ccatttcgcg gcttcgtagt gttccatatc
    27961 accatgataa tcaaggtgat cgcggcttaa gttggtaaag accgacgccg caaatttcaa
    28021 tgccgccaca cggtgctgta ccagcccgtg ggaggaaact tccattgcgc aaaacgtcgc
    28081 gccctgatcc accagccccg ccagctcatg ctgaacatcg actgccgaac cggtggtatt
    28141 ttctgtcggg atcactttcc ccagcagacc gttaccaacg gtgcccatta ccgcgctggt
    28201 ttcgccaagc agttggctcc actgcgccag cagctgggta gtcgtggttt tgccgttggt
    28261 gcccgttacg cctacgagac gtaaattgtc agagggttca tggtaaaagc ggcccgccag
    28321 tgcagataaa cgctcgttga gctggctgag atagatgacc ggtacgccgt gcatttcacg
    28381 gatttcaccg tcggtcgcct cacctttcgc ctctgcaata atggcagcca caccttgcgc
    28441 tatcgcctgc gggatatatc gacgcccgtc cgcctgatga cctactacag ctacaaagag
    28501 atcgcccgcc gcagccacac ggctgtcgag tgtcatctct cgcagtgctc gcgaaggtgc
    28561 gtctggcacc cacggagcaa gaaggtcgcg caaattacga tctgccacct gtcccctcgc
    28621 cttgattaat cacaaattca tttttatcgc ccgttgtcag cgcatccggc tcgatgttca
    28681 tggtacgcaa tacgccgccc atgatggcac caaagaccgg cgctgaaacg gcgccgccgt
    28741 agtatttacc cgcctgcgga tcgttgataa caacaaccag cgcgaagcgc ggctgactcg
    28801 caggcgcaac gcctgcggta taagcaatat atttattgat gtagcgaccg tccggcccga
    28861 cctttttcgc ggtaccggtt ttaatggcga tacgatagcc tttaatcgcc gccttcacgc
    28921 cgccgccgcc tggtagcgcc acgctttcca tcatatgcac cacagtgcgg acaatggatt
    28981 ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt ggtaatcgac agtgggcgat
    29041 aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa ctgtaacggt gttaccatta
    29101 gcccgtagcc gaaagagaag gtggccctct ctatgtcaga ccaccgttgt ttttgaggat
    29161 ataagccact gcgttctccg accaacccca aattggtcgc ttttcccagt ccaaaacgtg
    29221 agtaagtatc tactaacgct gaggacggca tcgctaacgc cagcttggaa acaccgacgt
    29281 tactcgactt ctgtaatacc ccggtcaggg ttaattcgct gtagcgtgcc acgtctttga
    29341 tttcgtggcc gttaattcga taaggaatgg tattgagtac cgagttttcc cgcaccacgc
    29401 cacgttgcaa cgcggtcatt accaccatcg gtttaaccgt tgagcccggt tcaaacacgt
    29461 cggtgatggt acggttacgc atcgcctctt tcggcgtgcc gctcagattg ttagggttgt
    29521 atgacgggct gttagccatc gccagcactt caccggtgtt gacatccacc agcacggcgc
    29581 taccagattc agccttgtta aaggccaccg cgttgttcag ttcgcgataa accagcgcct
    29641 gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc ctggctgtca gtagaagaaa
    29701 tatcttcaat tacgcgacca tagcggtctt tacgcacaat gcgctcaccc ggctgcccgg
    29761 taagccattt atcgaaactc ttctcaacgc cctcaatccc ttgactatcg acgttagtaa
    29821 agccgatgag gtgagcagtc acttcgccgg acggatagta acggcgagac tcttcacgca
    29881 gatgaatccc cggcagtttc agttttttga tgtagtccgc catgtcaggg ttcacctgac
    29941 gcgccagata aataaagcgc cctttcgggt tggcgttaat gcgggctgaa agctgatcca
    30001 gcggaatatt gagcgcgtta gccagcgcct tccagcggtc accgacgctg ataccgccag
    30061 cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac cggcacgctc accgctaacg
    30121 ggcgaccaga acggtcagta atcatgccgc gggaggtgga aacttgctga acgcgaagag
    30181 aacgcatgtc gccctctttc accagcatat ccggggagat aacttgtaac cacgctacgc
    30241 gtccgagcag aaaagccagc gccaggagaa tacagccgca taacaacgca aaacgccaac
    30301 tgataaagtt ggcatgttct tcctgacgtt ttggtttctg cgttttcgcc gctgctttca
    30361 tgcgtcgcgt ttatccttat ttttgcacta cgatattttc ttgtgacgga tcaacatgct
    30421 gcatttgcag cttttccgtg gcgatccttt ccacccggct atggtcgccg agcgcattct
    30481 cttcaaggat caggttgcgc cattcaatgt ctaaagcatc tcgctccagc accagttgtt
    30541 cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt taccacagtc accgccgtca
    30601 aaataatgca aatgaacagg cagagtggca gcttcccaaa tcgcaaaaga tcgtcaccga
    30661 taacaccaga caatgcatgg cgctcgtggc ttcccatcga tcctttaact ttgcttagag
    30721 cttctgtcac tctgctgatc atgcattcgt cctctctgca atacgcagaa ctgaactacg
    30781 ggcacgaggg ttctcagcca cctcttcttc gcccggcatt aacttgccta gtgctcgcag
    30841 ctgacggcca cccagttttt tgagctgctc ttcagtcatc ggtaaccctg ccggaacttg
    30901 cggaccgcgg ctgttttcac gcataaaacg tttcacaata cggtcttcca gcgagtggaa
    30961 gctgatgatc gaaagccgcc cacccggggc cagcacgttg agcgagcttt ttagcgcctg
    31021 ctctatctcc tccagttcac tgtttaccca aatgcgcacc gcctggaagg tacgggtcgc
    31081 gggatgttta aacttatctt tcaccggcgt tgcagcagcc acgacttccg ccagttcttt
    31141 ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg gcgcgggcaa tgcgtttggc
    31201 aaaacgctct tcaccatagg ttttcaatac ccaggcgata tcggcttctt ctgcggtttg
    31261 tagccattca gcggctgact gcccacgggt tgggtccata cgcatgtcca gcggaccatc
    31321 gcgcataaag gaaaagccgc gctcagcatc atcaagttgc ggtgaagaga cgccaagatc
    31381 gaggagaatg ccgtcgatct tgccgataag atcgcgctcg gcaacgtatt cgcccagcgc
    31441 ggagaaaggt ccgtggatga tggagaagcg cggatcatca atagtcttcg caacggcgat
    31501 agcctgcggg tcgcgatcga tcgccagcaa acgcccctct tcgccaagct gcgagaggat
    31561 cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg tagatgccat caggacggat
    31621 attgaggcca ttaacggctt catccagcag caccgtagta tgtttatagt tttccatcat
    31681 tttatagaga caagtcctgc agtcgctccg ataagtctcc ggtagccaac tgctctgcgt
    31741 cgatatcttc cttgacctgt tgatgccagg ttgtttcatc ccacagctca aacttgttga
    31801 actgtccaac cagcatcact tcttttgtca gcccggcatg ttgccgcagt actggcgcga
    31861 ttaacaatcg acctgcgcca tccatctgac attcgctggc atgacctaac agtaggcgct
    31921 gcacacggcg ctcaaccggg ttcatgctcg acagacgcga taatttttgc tcgataattt
    31981 cccattcagg cagggggtaa agcagcaggc acgggtgatg aatgtcaatg gtgcaaacca
    32041 tttgaccggc agcgttctca agcagctgtt cccggtaacg ggtaggcact gataagcgcc
    32101 ctttgctgtc gagattgact aacgttgctc cccggaacat gccagcctca ccccttattc
    32161 ccactttgcc ccacaatttc ccactgaaag gagtttacgg agcggaggaa aagcttgtca
    32221 agccagtcac aaggcatacc gaggtaaaaa acccgatgtt tacggctaat atcagcgttg
    32281 tttattttac gtccaacgtg cgcagcaaat taacgttatg aatagctgta agaaaaaatc
    32341 cctcatcgtc atcaccgttt taaaatgact cactattttc cacagcaaat ataaagtgtc
    32401 agtttgcgac gcgagcggca ttttaggaca tatcttcccc ggttaacagt ccctgttgcg
    32461 tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca aagaaaagtg cttgttaaat
    32521 cggcaattca tctctgcatg taacaaaata atgcaaaaac gcgctgtcga tatcacttaa
    32581 ttattgtgaa tttcagctgg tgagttaatt aacgggcggg tttaaaagaa cttatcaata
    32641 atagtcagga atagtcttat ttactttaag gatattgatg tccattcccg tactctacgc
    32701 gccagaggga aattcacctg gcgcgtattt ttgttcgcgg cttagctacg gctgagcaca
    32761 ccgcggcgat agagattacg tttaatgcgc gttaaaccag gttttggctt acgcggttcg
    32821 tccaggcttg ccaggacaat ctccagcaca cgctctgcga catcgcggtg acgttgagcc
    32881 actgccagca ccgggcactg taagaagtcg agcagttcgt tatcgccaaa ggtggcaatt
    32941 gccaggtcag aaggcagttt accatcgcga cgtagcgtga catccatcac cccttgcaac
    33001 aacgcaaacg acgttgtgaa cagcgcctgc ggcatcggat gcgtttccag ccatttttcg
    33061 aataactggg cagccgcctc ccgctcatag ctgttggcat acaggaaatg cacttcgcgc
    33121 ggatcatctt tccaggcagt acggaaacct tgttcacgca ggaagctgac agaaagctcc
    33181 ggcagcgcgc caagataaag caccgtctcg gcgggaaact tacgtaactc ttccgccagc
    33241 atttcggcat catcctgatc ggcaccaacc acgctggtga aatgttcacg atcgagggcg
    33301 cggtccagcg cgacaatcgg gaacgggtcg ttagcccagc gttgataaaa aggatgctca
    33361 ggtggcaacg acgtcgaaac aataatggca tcaacctgac gctgtaaaag gtgctcaatg
    33421 cagcgcattt cgttgtctgg ctgatcttct gagcaggcaa tcagcagttg ataaccccgt
    33481 tgccgcgcct ggcgctcaag atagttagcg atgcgggtat agctggtgtt ctccagatcg
    33541 gggatcacaa gaccaataga acgtgtgcgt ccagcacgaa gcccagctgc cacggcgttc
    33601 gggtggtaat tgtgctcacg caccacagcc atgacttttt caacggtttt gtcgctcaca
    33661 cggtattgct tcgctttgcc gttaataaca tagcttgcag tggtccgcga cactcccgcc
    33721 agccgagcga tttcatccag tttcacaatt gccccttgcg taaaatgtaa aaaccatagc
    33781 cattgtactg gtatgggtta aattcattaa catctaagcg cagaaaatta actgcggcaa
    33841 ctgcttttat tccaggttcc cactgatttc gcaaaaaaag cccaacgtga catgttgggc
    33901 tgcaaattgc gcactgagat cattctcaac gcattatttt atcgccgcgc gaaagtccga
    33961 ccacaccaga gcgagcaact tccacaattt tcgccacatc gcgaatcgat gctaaaaatg
    34021 catcaagctt atcgctggtg cctgctaatt gaacggtata aagcgagggg gtgacatcga
    34081 taatttgccc acggaatatt tccgtattac gtttcacttc gtcacgcccg taaccgctgg
    34141 cctgaatttt caccagcatg atttcccgct caacatgcgc gccctgcccc aactcactca
    34201 cgcgcaagac atcgaccagc ttgtgtaatt gcttttcgat ctgctcaagc actttttcat
    34261 cgcccacggt ctggatggtc atacgcgata atgtcggatc gtcggttggc gcaacggtca
    34321 ggctttcaat gttgtagcca cgctgggaaa aaaggccaat cacgcgggat aacgcgcctg
    34381 attcattttc gagtaagact gataatatcc ggcgcataat caggttctct ccgttttgct
    34441 taaccacatt tcatccattc cgcccccgcg aatctgcatc gggtagacgt gctcactgcc
    34501 atcgacggta acatcaacaa acaccaggcg attattgcac acctgttcca gcgcctcgct
    34561 aagtttgctt tccagctcat gcggatgaga gatctggatc ccgacgtgac cataggcttc
    34621 cgccagacgg acgaaatcgg gtagtgattg catataagat tgtgaatgac ggccggaata
    34681 gatcatgtcc tgccactgtt tcaccatccc cagatagcgg ttattgagat tcaccaccag
    34741 taccggcaac tcgtattgca acgcggtaga cagttcctgg atgttcatct gaatactgcc
    34801 gtcgccagtg acgcaaacca cggtttcttc tggcaacgcc attttgacgc ccagtgccgc
    34861 aggtaaacca aaacccatcg tgccgaggcc accggaattg atccagcgac gcggtttgtc
    34921 gaatggataa taaagtgcag caaacatctg gtgctgcccg acatcggacg tcacgtaagc
    34981 gtctcccttc gtcaaccgcc aaagagtctc gatcaccgcc tgcggtttaa tcttttcact
    35041 gtgagtgtca tatttcaggc actgacgagc gcgccactgt tcaatttgct gccaccagtc
    35101 gcggatctca tccagtggtt gatgggcgga ttcttgcgac aagagttcaa gcatttgttc
    35161 gaggacctgg cgagcatccc ccacaatcgg gatatccgca gtcacggttt tagaaatgga
    35221 agtaggatca atatcgatat gcagaacagt ggcatttggg cagtactttg ccagattgtt
    35281 cgtcgttcgg tcatcaaatc gtaccccgac ggcgaaaatc acatccgcgt tatgcatcgt
    35341 catattggct tcgtaggtac cgtgcattcc cagcatgccc agtgcctgac gatgcgttgc
    35401 cggaaacgcc cccagcccca tcaatgagga aacaacgggc agattcaacg cctccaccgt
    35461 ttctttcaac tgctgatggc agcccgccat gattgcccca ccgcctacgt agacaaccgg
    35521 tttttttgcc gctaccagcg tttgcagagc gcgcttaatt tgccctttat gtccggtagt
    35581 agtgggattg taagaacgca tactgaccga ctcaggccag acatagggta atttgttcgc
    35641 cggattaaga atatctttcg gtaaatcaac gaccactggt cctgggcgac cacttgccgc
    35701 cagccagaaa gcctttttca gcacctgcgg aatgtcttcc gtttgcttaa ccagaaaact
    35761 gtgtttaacc accggtcgcg aaatccccac catgtcgcac tcctgaaagg catcgtaacc
    35821 tatcaacgag gtcgctacct gcccggaaag gacaactaat ggaatggaat ccatataagc
    35881 ggtggcgatg ccggtaatcg cattggtcgc ccctggaccc gacgttacca gcacgacgcc
    35941 gacttccccg gtcgcgcgcg ccagaccatc ggccatatgc actgccgcct gctcatgacg
    36001 aactaaaaca tgatcgatac cacccacggt atgcaatgca tcgtaaatat caaggactgc
    36061 gcctccggga taaccgaata cttgtttaac gccctgatcg ataagcgatc ggacgaccat
    36121 ctcggctcca gacaacatct ccatggcctg cctcactgtt tgacggaaaa aatgtgtaaa
    36181 acacattaac cgtccgatct tgcttaagca attggcagcc agagacataa acgcagggta
    36241 ataagaataa acaggaggaa aggtgaaaag aaacgataaa atcctccatt acgaaggatt
    36301 aaattgataa taaatcggct gaatcccaca acttacagca atttctctat ttttttaatt
    36361 aatcctaaaa tagagagccc acacattcag aataaaattc tacattcaat aaaaaataca
    36421 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
    36481 actaattgct cttccatcca ctgatggcct ttatcgcgcc cggcagcttc atgccaggag
    36541 agataacagg ttctgctatt ttgttttaac ggcagcggta atacctgtaa ttctaaggat
    36601 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
    36661 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
    36721 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
    36781 tcgttataaa catcatgttt cagtaacggg cccttaattg ttggatgatt tttgctggct
    36841 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
    36901 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
    36961 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
    37021 tgcgaggtca gaatgctgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
    37081 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
    37141 actgagccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
    37201 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
    37261 gacataccca gaacatgagc ggcacgggta atgttttgct cctgcatcac ggcatcgaaa
    37321 acggttaata agttgagatc gaccatgcgt agctgtggct tgcttaactc cgccgtctct
    37381 gaatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
    37441 cttattggaa tgcagagaaa taatcctgaa gaatatgatt tatcatgcat ataaaataag
    37501 aaaaagcaaa atgagtaaaa ttcggaaaac ataaagatac gggttctgat aaaaacagaa
    37561 tccattgccg tggaatgagt catttaccac agcataataa tccataatg
//
LOCUS       DAPHJE010000039        37367 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPHJE010000039 DAPHJE010000000
VERSION     DAPHJE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.039"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..840
                     /gene="potE"
                     /locus_tag="RH222_001944"
     CDS             <1..840
                     /gene="potE"
                     /locus_tag="RH222_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDV3796974.1"
                     /translation="VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFL
                     GLESACANTDVVENPERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFG
                     LAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
                     APVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVA
                     NVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKN
                     KHG"
     gene            complement(896..2536)
                     /gene="pgm"
                     /locus_tag="RH222_001945"
     CDS             complement(896..2536)
                     /gene="pgm"
                     /locus_tag="RH222_001945"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV3796975.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(2562..3107)
                     /gene="seqA"
                     /locus_tag="RH222_001946"
     CDS             complement(2562..3107)
                     /gene="seqA"
                     /locus_tag="RH222_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV3796976.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3292..4056
                     /gene="ybfF"
                     /locus_tag="RH222_001947"
     CDS             3292..4056
                     /gene="ybfF"
                     /locus_tag="RH222_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3796977.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4196..4489
                     /gene="ybfE"
                     /locus_tag="RH222_001948"
     CDS             4196..4489
                     /gene="ybfE"
                     /locus_tag="RH222_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV3796978.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            4629..5159
                     /gene="fldA"
                     /locus_tag="RH222_001949"
     CDS             4629..5159
                     /gene="fldA"
                     /locus_tag="RH222_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV3796979.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5369..5455
                     /gene="uof"
                     /locus_tag="RH222_001950"
     CDS             5369..5455
                     /gene="uof"
                     /locus_tag="RH222_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV3796980.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            5448..5894
                     /gene="fur"
                     /locus_tag="RH222_001951"
     CDS             5448..5894
                     /gene="fur"
                     /locus_tag="RH222_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV3796981.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5979..6305)
                     /locus_tag="RH222_001952"
     CDS             complement(5979..6305)
                     /locus_tag="RH222_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3796982.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(6355..7761)
                     /gene="chiP"
                     /locus_tag="RH222_001953"
     CDS             complement(6355..7761)
                     /gene="chiP"
                     /locus_tag="RH222_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV3796983.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSPHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(8341..10005)
                     /gene="glnS"
                     /locus_tag="RH222_001954"
     CDS             complement(8341..10005)
                     /gene="glnS"
                     /locus_tag="RH222_001954"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV3796984.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(10273..12219)
                     /gene="nagE"
                     /locus_tag="RH222_001955"
     CDS             complement(10273..12219)
                     /gene="nagE"
                     /locus_tag="RH222_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV3796985.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVRAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            12552..13352
                     /gene="nagB"
                     /locus_tag="RH222_001956"
     CDS             12552..13352
                     /gene="nagB"
                     /locus_tag="RH222_001956"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV3796986.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            13412..14560
                     /gene="nagA"
                     /locus_tag="RH222_001957"
     CDS             13412..14560
                     /gene="nagA"
                     /locus_tag="RH222_001957"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV3796987.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            14569..15789
                     /gene="nagC"
                     /locus_tag="RH222_001958"
                     /gene_synonym="nagR"
     CDS             14569..15789
                     /gene="nagC"
                     /locus_tag="RH222_001958"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV3796988.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            15837..16589
                     /gene="nagD"
                     /locus_tag="RH222_001959"
     CDS             15837..16589
                     /gene="nagD"
                     /locus_tag="RH222_001959"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV3796989.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            16986..18650
                     /gene="asnB"
                     /locus_tag="RH222_001960"
     CDS             16986..18650
                     /gene="asnB"
                     /locus_tag="RH222_001960"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV3796990.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVTDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            19029..19105
                     /locus_tag="RH222_001961"
     tRNA            19029..19105
                     /locus_tag="RH222_001961"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19063..19065,aa:Met,seq:cat)
     gene            19114..19198
                     /locus_tag="RH222_001962"
     tRNA            19114..19198
                     /locus_tag="RH222_001962"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19148..19150,aa:Leu,seq:tag)
     gene            19222..19296
                     /locus_tag="RH222_001963"
     tRNA            19222..19296
                     /locus_tag="RH222_001963"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19254..19256,aa:Gln,seq:ttg)
     gene            19331..19405
                     /locus_tag="RH222_001964"
     tRNA            19331..19405
                     /locus_tag="RH222_001964"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19363..19365,aa:Gln,seq:ttg)
     gene            19421..19497
                     /locus_tag="RH222_001965"
     tRNA            19421..19497
                     /locus_tag="RH222_001965"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19455..19457,aa:Met,seq:cat)
     gene            19546..19620
                     /locus_tag="RH222_001966"
     tRNA            19546..19620
                     /locus_tag="RH222_001966"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19578..19580,aa:Gln,seq:ctg)
     gene            complement(19774..20949)
                     /gene="ubiF"
                     /locus_tag="RH222_001967"
     CDS             complement(19774..20949)
                     /gene="ubiF"
                     /locus_tag="RH222_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV3796991.1"
                     /translation="MTNQPTEIAIVGVGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            21095..22519
                     /gene="miaB"
                     /locus_tag="RH222_001968"
     CDS             21095..22519
                     /gene="miaB"
                     /locus_tag="RH222_001968"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV3796992.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            22672..23712
                     /locus_tag="RH222_001969"
     CDS             22672..23712
                     /locus_tag="RH222_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV3796993.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            23709..24176
                     /gene="ybeY"
                     /locus_tag="RH222_001970"
     CDS             23709..24176
                     /gene="ybeY"
                     /locus_tag="RH222_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV3796994.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQKESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            24266..25144
                     /gene="corC"
                     /locus_tag="RH222_001971"
     CDS             24266..25144
                     /gene="corC"
                     /locus_tag="RH222_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV3796995.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            25169..26707
                     /gene="lnt"
                     /locus_tag="RH222_001972"
     CDS             25169..26707
                     /gene="lnt"
                     /locus_tag="RH222_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV3796996.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(26785..28662)
                     /locus_tag="RH222_001973"
     CDS             complement(26785..28662)
                     /locus_tag="RH222_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDV3796997.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            29117..30025
                     /gene="gltI"
                     /locus_tag="RH222_001974"
     CDS             29117..30025
                     /gene="gltI"
                     /locus_tag="RH222_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV3796998.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            30195..30935
                     /gene="gltJ"
                     /locus_tag="RH222_001975"
     CDS             30195..30935
                     /gene="gltJ"
                     /locus_tag="RH222_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV3796999.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            30935..31609
                     /gene="gltK"
                     /locus_tag="RH222_001976"
     CDS             30935..31609
                     /gene="gltK"
                     /locus_tag="RH222_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV3797000.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTTSTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31609..32334
                     /gene="gltL"
                     /locus_tag="RH222_001977"
     CDS             31609..32334
                     /gene="gltL"
                     /locus_tag="RH222_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV3797001.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32452..33387
                     /gene="rihA"
                     /locus_tag="RH222_001978"
     CDS             32452..33387
                     /gene="rihA"
                     /locus_tag="RH222_001978"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV3797002.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            33471..35141
                     /gene="hscC"
                     /locus_tag="RH222_001979"
     CDS             33471..35141
                     /gene="hscC"
                     /locus_tag="RH222_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDV3797003.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDEARECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35201..36652)
                     /gene="djlC"
                     /locus_tag="RH222_001980"
     CDS             complement(35201..36652)
                     /gene="djlC"
                     /locus_tag="RH222_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDV3797004.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(36649..37356)
                     /locus_tag="RH222_001981"
     CDS             complement(36649..37356)
                     /locus_tag="RH222_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3797005.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9175 a         9622 c         9392 g         9178 t
ORIGIN      
        1 gtggtaggcc tgtgcattat cggctggttc tggtttagcc cgacgcttta cgttgattcc
       61 tggaatccgc atcatgcacc gttcttcagt gcagtaggtt cttccatcgc tatgacgctg
      121 tgggcttttc tcggtctgga gtctgcgtgt gcgaatactg atgtagtgga aaacccggaa
      181 cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc
      241 tccaccaacg tgattgcagg gattgtgcct aatatggagc tggcaaattc aacggcacca
      301 tttggcctgg ccttcgcaca gatgttcaca ccagaagtgg gtaaagtcat tatggcgctg
      361 atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt
      421 aaatcttcat ctgatgaagg ctacttccct aaaattttct cccgtgtaac caaagtggat
      481 gcaccggtgc agggaatgtt gaccattgtg attatccaga gcgggttggc gctgatgacg
      541 attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt ggtgaccaat
      601 atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg
      661 cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtatagcttt
      721 tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt aaccttcctt
      781 ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa acacggttaa
      841 aaaagcaaag taaaaaaggg cgatcttgcg accgcccttt ttttattaaa tgtgtttacg
      901 cgtttttcag aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt
      961 cttcaccgag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct gacggacgcg
     1021 cggcgaacca gccgttgtca gtcatcactt tcagaccgcc gatagaagca ccattgcccg
     1081 gtgccgccgt caggcgcgcg gtgatcgggt cgcctgccag ggtgctggcg ctcaccattt
     1141 ccggagacag cttagacagc gccgcttttt gtgcggaagt cgcagctgcc tgcaaacggt
     1201 tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc gggttcttac
     1261 cggtgaccgc ggtgatttcc gccgccagca gacacatgat gatgccgtct ttgtcggtgg
     1321 accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg ccgccaaaac
     1381 cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa acctaccggt acttctacca
     1441 gcttacggcc caagtcattg accacacggt cgatcatcgc agaagaaacc agcgttttac
     1501 cgacagcaac atctttgccc cactgcggac gatgctggaa caggtagttg atcgccaccg
     1561 ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat
     1621 ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg
     1681 cacactcgga ggagcagtcc atacggatcg cgccgtcttt atcaaggtgc ataaagcgga
     1741 aggtttgatc gacctgatcg ttaacgatgg tcaggttgag gttgtaatac tcgccaatac
     1801 gcttccagta ttcgataccg gaaccgccca gcggatcaac gcccagcgtc aggcccgctt
     1861 tctgaattgc cgccatatca acgatatcgg ccagaccttc cacaaacggc tgcaccagat
     1921 cctgctcttt tacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt
     1981 tcaggccatc ggccatcagt gcgttggccc tgtcttccac cactttggtg acgttggtat
     2041 cagccgggcc accatttggc gggttgtact tgataccacc atcttccggc gggttatggg
     2101 acggtgtaat cacgataccg tctgccagcg ggccaccttt tttattgtga accaggatgg
     2161 cattggaaat ggcaggtgtc ggagtgaagc cattgttttc ctgcacaatg acatcaacgc
     2221 cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat
     2281 ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct
     2341 gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac
     2401 cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat
     2461 attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac
     2521 gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc
     2581 ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat
     2641 ttacggccgg tgttggtgtt ggtgatcacc caatacggcg tgcctggcac atgtttcggc
     2701 ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta
     2761 cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga atatagtgta
     2821 gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc gtccgaaagc
     2881 agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg
     2941 atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg
     3001 gaaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg
     3061 ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc
     3121 agtgtcttag ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc
     3181 gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac
     3241 aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg
     3301 aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccat
     3361 ggtctgtttg gtagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac
     3421 aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagagcc ggtaatgaat
     3481 tacccggcga tggcgcagga tcttgttgat accctggatg cactgcagat cgacaaagca
     3541 acatttatcg gtcactccat gggcggtaaa gcggtaatgg cacttactgc actggccccc
     3601 gatcgcatcg ataaactggt ggcgatcgat atcgcaccgg tcgactatca cgtacgccgt
     3661 catgatgaga ttttcgcagc tatcaacgcg gtcagtgaat cggacgcaca aactcgccag
     3721 caagcagcag caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgttg
     3781 aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg tattgtggga tcagtatccg
     3841 catattgtgg gttgggagaa aatcccggca tgggatcacc ctgccctgtt tatccctggc
     3901 ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca atttccacag
     3961 gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg
     4021 gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgt
     4081 ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc gcgctgatgt attatggcgc
     4141 gctatccatc cgggcagagg cctggctgat tgtttccccc gaagtcacca agatcatggc
     4201 caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg
     4261 accgaaaact aatccgcttt cgcgcgatga acagctgcgt attaataaac gcaaccagct
     4321 aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg cggaagctgt
     4381 cgaggcgctg aacgagctgg cggagtcgcg caatatgagc cgtagcgaac tgatcgaaga
     4441 gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccacttt
     4501 catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct
     4561 atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta
     4621 tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata
     4681 tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa
     4741 aaagcagcaa agaagatctg gaagcttatg acattctgct gctgggcatc ccaacctggt
     4801 attatggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt
     4861 tcaatggcaa actggttgcg ctgtttggtt gtggtgacca ggaagattac gccgaatatt
     4921 tctgcgatgc attgggcacc atccgcgaca tcattgaacc gcgcggtgca acgatcgttg
     4981 gtcactggcc aactgcgggc tatcatttcg aagcatcaaa aggtctggct gatgacgacc
     5041 actttgtcgg tctggctatc gacgaagacc gtcagccgga actgaccgct gaacgtgtag
     5101 aaaaatgggt taaacagatt tctgaagagt tgcatctcga cgaaattctc aatgcctgat
     5161 gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt
     5221 gctacaaatt tgtaactttt gctgttgtac ctgtacaatg tcccggtgtt caagtggcct
     5281 tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgtg
     5341 gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag caaattctgt
     5401 cacttcttct aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca
     5461 ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag
     5521 ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg
     5581 atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgacg
     5641 ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc
     5701 aacatcacca cgatcacctg atctgcctcg actgcggcaa ggttatcgaa tttagtgatg
     5761 attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc
     5821 acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat gaacatgcgc
     5881 acgaaggcaa ataagccagc ctgaatgaga aaaagccaac ctgcgggttg gcttttttat
     5941 gcaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt
     6001 tcctgccaaa ctttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca
     6061 cgcagacact gctgataatc cagtacgcgg acactttcct ggtcggcaaa atgctgatgt
     6121 tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttctggcgaa
     6181 ccttgtgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc ttcctgtggc
     6241 gcggttgatt gcgcacaagc caccagcccc gatgccatca tggcgatgag aatcagtttt
     6301 ttcatagcaa attcctcaaa tgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa
     6361 gatggtgaat ggtgcgatta ccataaattt cacgtcacgc tcatcctgga agatgttgcc
     6421 gtaaccaccg ccccagcttg ggatgtcgga gtggttgtcg tattcggtga aatgcagttt
     6481 gaacatcgtg cctttggcgc gaccgtcctg aatggtgtag accgcatcca ggctgtatgc
     6541 agactcttca atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc
     6601 aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca
     6661 gtttttcagg tcatacatcg caccgaagaa gactgctttt tcgccgttgg cgttgaagtc
     6721 agaacggttg tcccaccaga tatccaggcg accgtttgag gaagcgtagg ttggagtcat
     6781 acgttgcagg aagtatccct gctgaccgtc agccttaacc caggtgcctt cgaggcgcaa
     6841 atccactacg tcagccgccc ggtaaccaaa ggtcaacgcc tgcagccagg cggtgccgtc
     6901 ataaaggtcg ttgacgctgc gatcgtcaac tttatcgcgg gtaccgtaga actggtagct
     6961 ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa aatattgatc
     7021 gatataccct tccgcctgac caaaagccgc ttccagtacg aagttatttt tgaagtcgta
     7081 tttcgcccca agggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc
     7141 atccatttcc agatgccacg gcgctttgta ttcgttggtc cacatgtagg agaaactcaa
     7201 cgcaccggca tcgccgtaat caaaattcgc tccggcttcc gcaccctgat aagtacccgg
     7261 cataaagctc cagtgaggcg ctaacagcgt ctgaccggtt ggctgaatat aacctgccct
     7321 cgcccaaacc ggaccgtatt taaatttggc cgctgcttta tacaggctta taccgctttt
     7381 gtcgccggac cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg
     7441 gtgagggctg tcgccgtttt ccgccatttc aatcgccgta aacgcggcaa tatcaaggcc
     7501 gaacatatca gcagcataac cagactgaaa atcgaggttg gcattccagg tggagtgaga
     7561 aaggttggtt ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca
     7621 gtaatagata ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg
     7681 cattgccata aagcccgaca ttgctgtaac accggcgata gccagcgcca gcgtactacg
     7741 tttgccacta aacgtacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag
     7801 gtgaacagcg aaaaaaaata aaaatttaaa aagcagcgtt gagaaaagtc tcgctgacta
     7861 caggaacaga ctatttttcg cgacgcgaat tatcaatctc tttctgtaac cagaatgtaa
     7921 gaacatgagc aaccgcacat attttgatga tttttgttac aaggcttaag tttgcggttg
     7981 aaaggcttgc agaagaataa aagcgtgaat ttccagcaag atgaaagcgg ttacatttta
     8041 tttaattatg ttttgttatt ttaaatttat tttatggata aattgcgaag caggattaat
     8101 tcgcgtagaa aagtagaaaa aaacgccaca gagcgcggcg ttttggattg tttgctgttt
     8161 cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa ctcaatatat
     8221 tgcagagatc atgtaggcct gataagcgta gcgcatcagg caatttagcg tttgaaactg
     8281 tttgtctgtt ccttgccgga tgcgacgtaa acgccccatc cggcatagcg aaacttaaaa
     8341 ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt
     8401 ttccgccgta gaatggcggc tatcgaggca gaagtaacct tcacgctcaa actggaatgc
     8461 tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga
     8521 ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag
     8581 acggtcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac
     8641 acctttgact ttacgaccat ctgccggatc tttgcttaag gtatcggcgt cataagtaca
     8701 gaagatggtg gtgatattac cttcggcatc tttctcgacg cgttctgcct taatcacata
     8761 agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc
     8821 ttcgcggaaa tcggcgcgat caatccaaat ctcaccgcta aacggcacct gacggctgcc
     8881 catttccggt ttgttcggat ggttcggcat ggtaaccatt tcgccttcgc cctgatagtt
     8941 ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag
     9001 atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac
     9061 gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc
     9121 ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag
     9181 gttcaactta cgcttggaca tcacggtgta ttccagattc aggcgcgaga actcatactg
     9241 gcgcgggtga acaggaatcg tgatgttgtc cagtacccag tcgtacagac gacggttgtc
     9301 ctggaactca agcgtacaca gagagtgcgt aataccttcc agggcatcgc tgatgcagtg
     9361 ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa
     9421 tttaatacgg tacagcaccg gatcgcgcat cacgataaac ggtgaagcca tgtcgatttt
     9481 cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc
     9541 caggttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggtt gcgtcagggt
     9601 gccgcggtat tcgcggatct gttccggcgt cagttcatca acgtacgcca ggcctttatt
     9661 gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt
     9721 accagaccag tgaaaaccta accactctac gtcgtttttg atcgactcaa catactcgat
     9781 atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg
     9841 ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg
     9901 ctccggcggg aaacgggtgt gtactgtggt gtgcttacca ctggccagat cttcatcgat
     9961 gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa
    10021 gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt
    10081 tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca
    10141 gagaagatct ccaccgcttt tgccagtcct gcgtgattgt cggatgcaac gctaaagcgt
    10201 cttatccaac ctggactgag cttttgtagg cctgataaga cgcgtcaagc gtcgcatcag
    10261 gcatgaagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    10321 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccggactaat
    10381 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    10441 tcttacctgc gcgccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    10501 gtcgataccc atgtggacga cgatctccgc gcctttttcg gtttccagac agaacgcgtg
    10561 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    10621 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    10681 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    10741 tggtacagcc tgcggttttg ctacaggagc ggcagttgcc ggagttgctt cagctgacgc
    10801 agctgctacc gggccacggg caacgacttt cttcatcgca tcgccaatgg attctgcttt
    10861 cgcgccaaca atcacctgaa tggtctgttt gttcagtttt actacaccag aagcacccag
    10921 acgtttacac atcgcatcgt tgacgcgagc tgagtcagcc acggtaaggc gcagacgggt
    10981 aatacaggcg tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    11041 tgccagttga gtcagacctt cttcagtatt gctgttagcc tcttcagtta cgatctcgtc
    11101 ttctttatct tcacgacccg gcgttttcag attgaacatg cggataacca aactgaacac
    11161 cacgaagtag atagcgaaga agacaacacc cataaccagc agcatccaga cgttctggct
    11221 ggcggccggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    11281 agcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    11341 gaggtacagc agcggtgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    11401 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    11461 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    11521 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    11581 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    11641 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    11701 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    11761 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    11821 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    11881 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    11941 ttctgggttg atggtcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    12001 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    12061 tgcgaggtta tcaaaaatcg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    12121 tggctgaccg aatcgcagca acagtgccgc caccggcagc accgcgatag ggagctgtaa
    12181 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    12241 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattgtactg ccgatgttct
    12301 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gcatcgcaaa
    12361 ttaaatgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    12421 tggctaaacc agaaaactta ttttatcatt caaaaaatca ggtcggattg acgcctgtct
    12481 gcgcaaatcc aggttacgct taaagatgcc taatccgcca acggcttaca ttttacttat
    12541 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    12601 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccgac tgccgatcgt ccgtttgtac
    12661 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    12721 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    12781 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    12841 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgacgccg
    12901 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    12961 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    13021 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    13081 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacattg ctggatgccg
    13141 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcactgcag gctgcagttg
    13201 aaggttgcgt gaaccacatg tggaccatca gctgtctgca actgcatcca aaagcgatca
    13261 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    13321 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ctccttgctc
    13381 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    13441 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    13501 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    13561 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    13621 caccgctgaa gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    13681 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    13741 cgtgcgcgtt atgcgcgagt acctggcaaa acatcccaat caggcgttag gtctgcatct
    13801 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    13861 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    13921 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    13981 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg
    14041 gattaccttt gccacccatc tgtacaatgc gatgccgtat attaccggtc gtgaaccggg
    14101 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    14161 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    14221 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    14281 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    14341 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    14401 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg
    14461 cacactcgcc gcaggtaaag tagccaacct gaccgcattc acacctgatt ttaaaatcac
    14521 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    14581 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    14641 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    14701 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    14761 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    14821 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    14881 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    14941 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca acgcaagctg
    15001 cgcgagctga tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    15061 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actggaagaa
    15121 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    15181 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    15241 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggcaa cgtcggtgaa
    15301 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    15361 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    15421 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    15481 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggcaaa
    15541 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttatcgc tggtgaaatc
    15601 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaggcgctg
    15661 aaggcgtttc gcactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    15721 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    15781 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    15841 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    15901 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    15961 ccaactatcc ttcgcagacc gggcaggatc tggcgaaccg ctttgccact gccggtgtcg
    16021 atgtacctga cagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    16081 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    16141 ccggtttcac tattaccgat gtgaaccctg attttgtgat tgttggcgaa acgcgttcct
    16201 acaactggga catgatgcat aaagcggcct atttcgtcgc taacggtgca cgttttatcg
    16261 ccaccaaccc ggacacccac gggcgcggtt tttatcccgc ctgtggcgcg ttgtgtgcag
    16321 ggatcgaaaa aatctccggg cgtaaaccgt tctatgttgg taagccgagt ccgtggatca
    16381 tccgcgcggc attaaacaaa atgcaggcgc attcggaaga aacggtgatt gtcggcgata
    16441 acctgcgtac cgatattctg gccggcttcc aggcaggtct ggagacgatt ctggtgcttt
    16501 ctggtgtttc gtcgctcgac gatatcgaca gtatgccttt ccgccccagc tggatttacc
    16561 cgtcagtcgc tgaaatcgac gttatctgaa tagcaaccac gtctcaggac gtggttggct
    16621 ctgatgacaa acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc
    16681 aatatattga attttcatgc ttttgtaggc cggataaggc gttcacgccg catccggcat
    16741 gaacaaatcg cactttgtca gcaatttggc ccccgtaaaa tataaacatt caataaaacc
    16801 cgctaaaaat tatcgattca tcaataaata tatcctcaca ccagaccttt tttcaccaat
    16861 cgacaatcac cattacgttt tttatttttt caccgccaaa tcgcttgcgc acagtgctgc
    16921 tttgcggcat ttttttaaac aagcaaacac aacaagcaac aaatacaagg ttaacggaga
    16981 aggttatgtg ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta
    17041 agaaagccct cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt
    17101 atgccagcga taacgccatt ctcgcccacg aacgtctgtc aattgttgac gttaacgcgg
    17161 gggcgcaacc tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct
    17221 acaaccacca ggcattgcgc gccgaatatg gcgatcgtta ccagttccag accgggtctg
    17281 actgtgaagt gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc
    17341 agggcatgtt tgcctttgca ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg
    17401 accatctggg gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg
    17461 cctcagaaat gaaagcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga
    17521 gctatttgtg gagccaggac ggcgaaatcc gttcttacta tcatcgcgac tggttcgact
    17581 acgatgcggt gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt
    17641 cagttaaaag ccatctgatg tctgatgtgc cttacggtgt gctgctttct ggtggtctgg
    17701 attcctcaat tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg
    17761 aacgctctga agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac
    17821 cggatctgaa agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc
    17881 acttcactgt acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt
    17941 atgatgtgac cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg
    18001 cgatgggcat taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc
    18061 tttacttcca caaagcgccg aacgccaaag aactgcatga agagacggtg cgtaaactgc
    18121 tggccctgca tatgtatgac tgcgcccgtg ccaacaaagc gatgtccgcc tggggcgtgg
    18181 aagcgcgcgt tccgttcctc gataagaaat tccttgatgt ggcgatgcgt attaacccac
    18241 aggataaaat gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgaag
    18301 cgtatctgcc tgcaagcgtg gcctggcggc agaaagagca gttctccgat ggagtcggtt
    18361 acagttggat cgacaccctg aaagaagtgg cggcgcagca ggttaccgat cagcaactgg
    18421 aaactgcccg cttccgcttc ccatacaaca cgccaacctc taaagaagcg tatctgtacc
    18481 gggagatctt tgaagaactg ttcccgcttc cgagcgccgc tgagtgcgtg cctggcggtc
    18541 cttccgtcgc gtgttcttcc gctaaagcga tcgaatggga tgaagcgttc aagaaaatgg
    18601 acgatccatc tggtcgtgcg gttggtgttc accagtcggc atataagtaa gaaatgcgaa
    18661 acagccccgg tgaaatccgg ggctgtttca gttattgagg tgcttgttga ttctgatatg
    18721 cagtaaggaa atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca
    18781 gtatttcata gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtatgac
    18841 aaaagaatgg ctgtagggtg ggaaaaaaca caaagtaaac aataattgac gaatatagcg
    18901 ccacgctgtt cgcaacctaa ccaaacagtc actttcgagc aattttcctt gaaaaagagg
    18961 ttgacgctgc aaggctctat acgcataatg cgccccgcaa cgccgataag gtatcgcgaa
    19021 aaaaagatgg ctacgtagct cagttggtta gagcacatca ctcataatga tggggtcaca
    19081 ggttcgaatc ccgtcgtagc caccatcttt tttgcgggag tggcgaaatt ggtagacgca
    19141 ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa gtctcgcctc ccgcaccatt
    19201 caccagaaag cgttgtacgg atggggtatc gccaagcggt aaggcaccgg tttttgatac
    19261 cggcattccc tggttcgaat ccaggtaccc cagccatctt cttcgagtaa gcggttcacc
    19321 gcccggttat tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct
    19381 ggttcgaatc caggtacccc agccatcgaa gaaacaatct ggctacgtag ctcagttggt
    19441 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat
    19501 tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag
    19561 cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca
    19621 cattaaaaaa gctcgcttcg gcgagctttt tgcttttctg cgttcattca atgtcgaatg
    19681 cgatgttgac acgtcttatc cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc
    19741 tacggttggc acgcatccgg caatgttgta aggctacaac cctaacgcat atttcagcgc
    19801 ctgacgtttc aacacgccag cacgctccgc cgccattaac ccgagattac gcataaaacg
    19861 cagtggtggc agattattgc tgaatccggc ataaaacaga tccataccgc tttgcataat
    19921 gaagttatcc gccatgcgcc gcatctggta acgcttgagg attggataac tggcccacgc
    19981 ttcgccgtag ctgcgggcgt tgaccagaac atcaatcagg gcatcgacat cacgataacc
    20041 aagattcacc ccctgccccg ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc
    20101 aagccctggc tgcacatact gcaacgcatg gcgacgcgtc agcggaaacg caccagcggc
    20161 aagcggtgta acgtaaccca gacgcgacgg gaaatgcttc gcgatttccg cctgtaactg
    20221 cgccatgttc atattctgca actggcgaat acgcgccgga gagtcatacc acaccagcga
    20281 tgcccagtta tcaaacaacg gcagaaacgc acgcggtccg tccggagtaa attgctgcca
    20341 ggtgctgtcg ccgggatcgt tctcacactg tacgctaatc aacatacacg actgcgcata
    20401 ctgccaggca tgaacgccaa tacccgccat ctgccgcacc tgcgaatttg cgccgtcggc
    20461 accaatcacc agcttcgcgc ggatcgtttc tccacccttc agttccagct cctgaagatc
    20521 atcatggcga tgcagcgcaa tcagtgagcc tggcacacgt aacgttactt tcggatgcgc
    20581 ttccagcgcc tgccacagcg cctgttgcag gacggtgttt tccaccatat agccaagcaa
    20641 cggtagctta agttcagcgg cgtcaaacac cacatgcgcc gtttcccact cccacgtttc
    20701 cagtctgcgg taaggatggc aacgcatagc ctgtactgca tcccagaccc ctaacccttt
    20761 aagcaatgat accgaagccg cgctgatcgc cgagatccgc acatccggtt ggctatcagc
    20821 gacaaacggt gcaggttctg cgtgctcgat caccgttacc gtaaatccgt gctgtgccag
    20881 ccccagcgcc agtgcgccgc cgaccattcc tacgccgaca atggcaattt ccgttggttg
    20941 atttgtcatg gtcaatcatc ctgttagcaa tatgctttaa gtttacagga tttttacccc
    21001 caagggctga taacgctcat actggtcaca acggcagcaa agcattacac tatgcgccct
    21061 gcattcctgg ctactatttc gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac
    21121 ctggggctgt cagatgaacg agtacgattc atcgaagatg gccgatctgc tggatgccac
    21181 ccacggctat caactgaccg acgtggcgga agaagcggat gtgctgctgc tgaacacctg
    21241 ctcaatccgc gagaaggctc aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt
    21301 aaaagagaag aatccagacc tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg
    21361 cgagcacatt cgccagcgcg cccactatgt cgatattatt tttgggccgc aaacgctgca
    21421 tcgtctgccg gagatgatca actccgtgcg cggcgaccgc agcccggttg tagatatcag
    21481 cttcccggaa atcgagaagt ttgaccgtct gccggaaccg cgcgccgaag ggccgaccgc
    21541 gtttgtctcc atcatggaag gctgcaataa atattgcacc tactgcgtgg tgccttacac
    21601 ccgtggtgaa gaggtaagcc gtccgtccga cgatattctg tttgaaattg cccagcttgc
    21661 ggctcagggc gtgcgtgaag tcaacctgct cggtcagaac gtgaacgcct ggcgtggtga
    21721 aaactacgac ggcaccactg gatcgtttgc cgatctgctg cgtctggttg cggcaatcga
    21781 cgggatcgat cgtattcgct ttaccaccag ccatccgatc gaattcaccg acgatatcat
    21841 cgaagtgtat cgcgacacgc cggagctggt gagcttcctg catctgccgg tacagagcgg
    21901 ttccgatcgc attctgaacc tgatgggccg tacccatacg gcgctggagt acaaagcgat
    21961 catccgtaaa ctgcgtgcgg cgcgtccgga tattcagatc agttctgact tcatcgttgg
    22021 cttccctgga gaaaccaccg aagacttcga gaaaacgatg aagctgattg ccgacgtcaa
    22081 tttcgacatg agctacagct ttatcttctc tgcacgtcca ggaacgcctg ccgctgatat
    22141 ggttgatgat gttccggaag aagagaagaa gcagcgtctg tatattctgc aagaacgtat
    22201 caatcagcaa gcgatggcat ggagccgtcg gatgctcggc accacccagc gtattctggt
    22261 agaaggtaca tcgcgtaaga gcatcatgga gctttccggg cgtaccgaaa ataaccgtgt
    22321 ggtcaacttc gagggcacac cggatatgat cggtaaattc gtcgatgtag aaattaccga
    22381 cgtctacccg aactctctgc gcggtaaagt ggtgcgtact gaagatgaaa tgggtctgcg
    22441 cgtggcagaa acacccgaat cggtgattgc ccgtactcgc aaagaaaatg accttggcgt
    22501 gggttattat cagccgtaat tctcaggccc gccgttccgg tgggccttgt tttttctctc
    22561 tttatcccca tctttatcgc aatgcttgcg cctttttcct gtggcgtgaa taatttcctt
    22621 ataggccggt gaatgttcag cttccggcgg catacagata agaggaacgg tttgaacata
    22681 gacactcgcg aaatcaccct ggagccagca gacaatgcgc gtctgttgag cctgtgcggc
    22741 ccgtttgatg acaacatcaa gcagctcgaa cgtcgtctcg gcatcgagat caatcgccgc
    22801 gataaccact ttaaactgac cggccgtccg atttgcgtca ccgctgcggc agacattctg
    22861 cgtagcctgt atgtcgatac tgccccgatg cgcggtcaga ttcaggatat cgaaccggaa
    22921 cagatccacc ttgcgattaa agaagcgcgg gtactggagc aaagcgcgga gagcgtgccg
    22981 gagtacggca aagcggtcaa tatcaaaact aaacgcggcg taattaagcc gcgcacgcca
    23041 aaccaggcgc agtacatcgc caatattctc gaccatgaca ttaccttcgg cgttggcccg
    23101 gcgggtacgg gtaaaaccta cctggcagtg gctgcggcag ttgatgccct ggagcgtcag
    23161 gaaattcgcc gtattctgct gactcgtccg gcagtagaag ccggtgagaa actgggcttc
    23221 ctgcctggcg atttaagcca gaaagtagac ccgtatctgc gcccactgta cgacgcgctg
    23281 tttgaaatgc tgggctttga gaaagtcgag aaactgattg agcgcaacgt tattgaagtc
    23341 gcgcctctgg cctatatgcg tggtcgtacg ctgaatgacg cctttatcat tctcgatgag
    23401 agccagaaca ccaccatcga acagatgaag atgttcctga cccgtatcgg ttttaactca
    23461 aaagcggtta tcaccggcga cgtcacacag atcgacctgc cgcgtaatac taaatcaggt
    23521 ttgcgtcatg ccatcgaagt gctggccgat gtcgaagaga tcagctttaa cttcttccac
    23581 agcgaagacg tggttcgtca cccggtggtg gcgcgtatcg ttaacgctta tgaagcctgg
    23641 gaagaagcag aacaaaaacg aaaagcggcg ctggcggcag aacgcaagcg cgaagaacag
    23701 gaacaaaaat gagtcaggtg atcctcgatt tacaactggc atgtgaagat aattccgggt
    23761 taccggaaga gagccagttt cagacatggc tgaatgcggt gatcccgcag tttcagaaag
    23821 aatcggaagt gactattcgc gtggtcgata ccgccgaaag ccacagtctg aatctgacct
    23881 atcgcggtaa ggataagccg accaacgtgc tctccttccc gtttgaagtg ccgcctggca
    23941 tggagatgtc gctactgggc gatctggtta tctgccgtca ggtggttgag aaggaagctc
    24001 aggagcaagg caaaccactg gaggcgcact gggcgcatat ggtggtgcac ggcagtctgc
    24061 atttgttagg ttacgatcac atcgaagatg acgaagcaga agaaatggaa gccctcgaaa
    24121 cagagattat gcttgctctg ggctatgagg atccgtacat tgccgagaaa gaataatttg
    24181 ccaggcattt gactggcaga cgtccccgcc gttggtgatt aacgacggcg ttttgattaa
    24241 ctaacatgag aacccatacg acgccatgag cgacgacaat tcacacagta gtgacacgat
    24301 aagcaacaag aagggatttt tctccctgtt actcagccaa cttttccacg gtgaaccgaa
    24361 aaaccgtgac gaactgctgg cgctgatccg tgattccggg cagaacgacc ttatcgacga
    24421 agatacgcgc gatatgctcg aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat
    24481 catgatcccc cgctcccaga tgattaccct gaaacgcaac cagacgctgg acgaatgcct
    24541 tgatgtcatc atcgagtccg cccactcacg tttcccggtg attagcgaag acaaagatca
    24601 cattgaaggg attctgatgg cgaaagactt gctgccgttt atgcgcagcg atgctgaagc
    24661 cttcagcatg gacaaagtgt tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga
    24721 ccggatgctg aaagagtttc gctctcagcg ttaccacatg gcgatcgtca ttgacgaatt
    24781 cggtggggtt tccggtctgg tgaccattga agacatcctg gaactaattg ttggtgagat
    24841 tgaagacgag tatgacgaag aagatgatat cgacttccgt cagctgagtc gtcatacctg
    24901 gaccgtgcgc gcactggctt ccattgaaga cttcaacgaa gcgttcggca cccactttag
    24961 cgatgaagag gttgacacta tcggtggtct ggtgatgcag gcatttgggc atcttccggc
    25021 gcgtggcgaa actatcgaca tcgacggtta ccagttcaaa gtggcgatgg ccgacagtcg
    25081 gcgtattatt caggttcatg tcaaaatccc ggatgactca ccccagccaa agctggatga
    25141 ataaaaccga aactggatag ataactacat ggcttttgcc tcattaattg aacgccagcg
    25201 cattcgcctg ctgctggcgt tattattcgg tgcctgcgga acgctggcct tctctcctta
    25261 cgacgtctgg cctgcggcga ttatttcgct gatggggctt caggcgttga cctttaaccg
    25321 tcgcccactc cagtctgccg ctattggctt ttgctgggga tttggcctct ttggcagcgg
    25381 tattaactgg gtctatgtca gcatcgcgac ctttggcggg atgcctggcc cggttaacat
    25441 cttcctggtg gtgctgctgg cggcgtattt gtcgctgtat accggactgt ttgctggcgt
    25501 gctgtcgcgt ctgtggccga aaaccacctg gctgcgcgtg gcgattgccg ccccttctct
    25561 ctggcaagtg accgagtttc tgcgcggttg ggtactgaca ggcttcccgt ggttacagtt
    25621 cggctatagc cagattgatg gcccgttaaa agggctggca ccgataatgg gcgtggaagc
    25681 cattaacttc ctgctgatga tggttagtgg cctgctggca ctggcgttgg tcaaacgcaa
    25741 ctggcgtccg ctggtggtgg ccgtcgtgct gtttgcccta cccttcccgc tgcgttacat
    25801 ccaatggttt accccacaac cggagaaaac cattcaggtt tcgatggttc agggcgatat
    25861 tccgcaatca ctgaaatggg atggagatca gcttcttaat acgctgaaga tttactacaa
    25921 cgcaacggct ccgctgatgg gcaaatcatc gttgattatc tggccggagt cggcgataac
    25981 cgatctggaa attaatcagc aaccattcct caaagcactg gacggtgagt tgcgcgataa
    26041 aggtagctcg ctcgtaaccg ggattgtcga cgcgcgtctc aataagcaga accgctacga
    26101 tacctacaac accatcatca cactgggtaa aggtgcaccg tacagctacg aatcagccga
    26161 tcgctataac aaaaaccatc tggtgccgtt tggcgagttt gtcccgctgg agtcgattct
    26221 gcgtccgtta gcaccgttct ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat
    26281 ccagccgccg ctgtcggtaa atggtattga gcttactgcg gccatttgct acgagatcat
    26341 tcttggcgag caagtgcgcg ataacttccg cccggatacc gactatctgc tgactatctc
    26401 caacgatgcg tggtttggta aatctattgg tccatggcaa cacttccaga tggcgcgaat
    26461 gcgtgcgctg gagctggcgc gcccactgtt gcgcagcacc aacaacggca ttacggcggt
    26521 gattggcccg cagggtgaga ttcaggcgat gatcccgcag ttcacccgcg aggtgttgac
    26581 cactaacgtg acgccgacca ccggactcac accgtatgca cgaaccggca actggccgct
    26641 gtgggtgctg acggcactgt ttggttttgc cgctgtgctg atgagtctgc gagcacgtaa
    26701 acgttgatgt gcctgatgta tcctgcatga gcaaaacgta ctttctcatt gagctttggg
    26761 gcatggttat catgcccctt tttcttacgg ttcagggata caagccacat tgccatattg
    26821 gggatgacga ataattgcct gcggatcaag cgagcgcgtc ctgtccagtt ggatcaaccc
    26881 gttcttcgcc ataacaaata tttttctacc atccgctgaa aaacgaaggt gattaacgta
    26941 gctggcagga taaacgccaa taatctgccc ggcattaata ttgagcagga tgacataacc
    27001 ttgcaggcca tatgacacca acgctaaata ctgaccgtcc gggctaatac cctcaacgga
    27061 ataaacgctc ctgctacacc attgcgtcat aatgctggct tgttgatccc ctacgcttat
    27121 acgtgaaatc ccctggctgt cggcaaaaat tacatccccg ttttcaggtc gaaaacgcat
    27181 cgccgatatc gttcctcgcg catcactagc cggatagctt tctttctgat gcgtgatggt
    27241 attgatgcgt acaatgtcac ctctgaccat accgggtatc aacaatattt tttcatccgg
    27301 ggaaagagcc ataacccagg caccaggttt attactcgtc cccgtcccgt cgtatttctc
    27361 cagcgggata tccgccacat gctgataagt caccagatca atcgctgaca cactattact
    27421 ggtgcgattc atcacccagg cttttgtgcc atcacgcgag aggataaggc tgtcaggatg
    27481 tgcaccggtg gtaatcgact gaataatctc ccctgtcgcc acgttaatga ccgataacgc
    27541 attcgcttca aatgaagtcg cataaatcaa cttttcatcc gggcttaatg ccagcgatct
    27601 gacgccctga tctttacagc caccacaatg tttgactgac ttttctttcg ctatgggcgc
    27661 tgtaaaggta tgcaggattt tcttttgtcc gaggtcgaaa acaacaatgc tgttcgtgtt
    27721 ttcaattgcg gcatacaaca tattaccatc ccgactgatc gccatatcat ggatatcggc
    27781 aatctcagcg agcaaatcac cactggcctg ttcacgactt accggagcgg ataatgtttc
    27841 cctgagggcg ttgcgttcct ccgctaactg ctgctttttt tgccgtcgct cttgttcaat
    27901 ctcctgtgga taaaacgctt cacgcaggct ttgcctgact tgtaaaactg actcatccat
    27961 ctgctgctgc gcaagccaga tagccccggt cagaagaaga ctgcccgcaa caataatccc
    28021 gccttccgta acgagtcggt tttgcccaga taaacgcgcg ctcagccaac ccaacgcgcc
    28081 acccgcaatc aggccgccaa tgtgacaagc attatctatt cctgtttgcc gggcaccata
    28141 caacagtgtc agcgccacca tacccaacag agaaaatact cgcctctggt ttttatggta
    28201 tgtgcccgca cctgaagcaa actgtgttgc tatcgctgca ccagctattc ccagaatcgc
    28261 cccggatgcc ccaacgctga tgactatctg ctcactttcg cgtaacgcat aacaggcgct
    28321 gatgagccca ccaaagacgc cggagaataa ccaggtaatc aggagtcgcc atttccccag
    28381 aatctgttcc gccacgcctc ccacgaccag gaatgcaaac atattcatca gcagatgaga
    28441 aacactactg tgcagaaaga tactgctcaa taagcgccag ggttcacccg acaaagtaag
    28501 aggcgcaacg tttgcgccga gatggataag tgcggattga tcgacctctt cataactcat
    28561 ccattgtgcg agataacaaa atatcccaat acagagtgca aaaaggataa gggttattgc
    28621 gggtaattga acatttaacg gcttaaccga agatgcagac ataaggtgtt tcttccttga
    28681 aaagacaggt aaaaagcatt tttcccgtgt gcagttatca tcccggcaaa cgtcccatgc
    28741 cgaaaagata attctgtccg aaatatgact ttttcagcct aaaattacaa tcgtaattaa
    28801 ttcgccccta agtatttttt ggcatgtaag ttgctttatt tactgtgtaa gcgatgaaat
    28861 cagccgcgac gcaaccaggt tgcaccacca caaatcagcg gtagcaccaa aaaggtgcgg
    28921 tttaagaatt ttgcttcgga atggtgcggc gcatctcgca aaaacaaaca ataaaacagc
    28981 aatatcttta catttagcca aactaaatgt tacctaacag gcacaacact gcacaataaa
    29041 gttgcagacg ataacaacac aaacactcac aacgggtatc catgcgttct taacgcagaa
    29101 gataaaggag ttggatatgc aattacgtaa acctgccaca gcaatcctcg ccctggcgct
    29161 ttccgcagga ctggcacagg cagatgacgc cgccccggca gcaggcagta cgctggacaa
    29221 aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgaa tcttcagtac ctttctctta
    29281 ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat tactccaacg ccattgttga
    29341 agcagtgaaa aagaaactca acaaaccgga cttgcaggtg aaactgattc cgattacctc
    29401 acaaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg gttctaccac
    29461 taacaacgtc gaacgccaaa aacaggcggc tttctctgac actattttcg tggtcggtac
    29521 gcgcctgctg accaaaaagg gtggcgatat caaagatttt gccgacctga aagacaaagc
    29581 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
    29641 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
    29701 aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg aacgtgcgaa
    29761 agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg aagcctacgg
    29821 ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca ctatcgctca
    29881 ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa atccaattcc
    29941 gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga
    30001 accgaatgac aaggcactga actaattaca agaaccaggg gcggaaattc cagccctctc
    30061 gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc gggcctgaaa
    30121 accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta ccctcttttt
    30181 tctggagtag atttatgtct atagactgga actggggtat ttttttacaa caagccccgt
    30241 tcggcaacac cacctatctt ggttggatct ggagtggttt tcaggtgacg atcgctttgt
    30301 cgatctgcgc ctggattatc gctttcctcg tcggttcatt tttcggcatt ttacgtaccg
    30361 ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc
    30421 cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg ccggagaaaa
    30481 tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt tcatccatgc
    30541 tctgcctggg gctgtttacc gccgcccgtg tttgcgaaca ggtacgcgcc gctattcagt
    30601 cgctgccgcg tgggcaaaag aatgccgcgc tggcgatggg cctgacgcta ccgcaggctt
    30661 atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg acctcagaga
    30721 tgatgaacct ggtgaaaaac tccgccatcg cctcgactat tggtctagtg gatatggcgg
    30781 cgcaggcggg taaattgctt gattactcag cccacgcctg ggagtcgttt accgccatca
    30841 cgctggcgta tgtattgatt aacgctttta tcatgctggt gatgacgctg gttgaacgta
    30901 aagttcgcct gcctggcaac atggggggta aataatgtac gagtttgact ggagttccat
    30961 tgtcccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa tcaccgtcac
    31021 ggcagtcgtg attggtattt tgtggggcac catgctggcg gtgatgcgct tgtccagctt
    31081 tgcgcctgta gcgtggtttg ccaaagccta cgttaacgta ttccgctcga ttcctttagt
    31141 tatggttttg ctgtggtttt acctgattgt gccgggtttt ctgcaaaacg tgctgggatt
    31201 atcaccaaaa aatgatatcc gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc
    31261 ggcctactat tcagagatta tccgtgctgg tattcaaagt atttctcgtg gtcagtcgag
    31321 cgccgcgctg gcgttgggaa tgactcactg gcagtcgatg aaactgatta ttctgccgca
    31381 ggcgttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt tccaggatac
    31441 ctcgctggtg tatgtgttat ctctggccga tttcttccgt accacctcaa ccattggtga
    31501 gcgtgatggt acgcaggttg agatgatcct gtttgccgga tttgtttatt tcgttattag
    31561 tcttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat gattaccctg
    31621 aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc aaccgaagtg
    31681 aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc aacgctgatt
    31741 aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga tggtatcgtg
    31801 gttaatgaca agaaaaccga tctggcaaag ctgcgttccc gcgtcgggat ggtattccag
    31861 catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa
    31921 gtgcttaaac gcgataaagc gccggctcgt gaaaaagcat tgaaattgct ggagcgcgtg
    31981 ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca gcagcagcgt
    32041 gtggcgatcg ctcgcgcatt gtgtatggat cctattgcga tgctgttcga cgaaccgacg
    32101 tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga actggcgaac
    32161 gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa agtggcgaat
    32221 cgggtgatct ttatggatga gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc
    32281 gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca ttaatcactc
    32341 tggcgcgcat acccaagtgc gcgccatctc acgctttatc gtttcgcccc tcgtatctgt
    32401 tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac aatggcactg
    32461 ccaattctgt tagattgcga cccaggtcat gacgacgcta tcgcaatagt tctcgccctc
    32521 gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca gacaccagaa
    32581 aaaaccttac gcaatgttct tcgtatgctg accttgctca atcgcaccga tattccggta
    32641 gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa tgtgcacggc
    32701 gaaagcggtc tcgacggccc ggcattaccg gaaccggcgt tcgcaccgca aaactgtacg
    32761 gcggtagagc taatggcgaa aacgctgcgt gaaagtgcgg aacctgtcac gattgtgtct
    32821 accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact gcatagcaaa
    32881 attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac gcctgcggct
    32941 gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc agggatcccg
    33001 gtggtgatgg ctggtctgga tgttactcac aaagcacaaa tccacgttga agacactgag
    33061 cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct ggatttcttc
    33121 ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca tgacccatgc
    33181 accatcgcct ggctgttgaa accggagttg tttaccactg ttgagcgctg ggttggcgtg
    33241 gaaacacagg ggaaatatac tcagggtatg acggttgttg attattatta tctgactggc
    33301 aataaaccga atgccaccgt aatggtcgat gttgatcgtc agggctttgt tgatttactg
    33361 gctgatcgtc tgaaatttta cgcttaacac acgtcattga ttaagatcac cacgcgttac
    33421 ctgggtggtc ttaatctgtt tatctctttt tctttcaaga tataaatgca atggataatg
    33481 cagaactcgc cattggtatc gatctcggta ctaccaatag tttaattgcc gtctggaaag
    33541 acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca tccataatta
    33601 gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc
    33661 atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc aactggcggt
    33721 taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc tcattaaaag
    33781 aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc gttccggctt
    33841 atttcagcga tgaacaacgc aagcataccc gtttagcagc ggagttagcc gggttaaatg
    33901 cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg catacccaac
    33961 aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt acggtgcttg
    34021 agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc ctcggtggcg
    34081 aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc accaggacca
    34141 cgcttaacga gagtgaactg gcagccttgt atgcctgtgt ggaagcggca aaatgtagca
    34201 atcaatcgcc attacacatt cgctggcagt accaggatga agcacgggaa tgcgaatttt
    34261 atgagaatga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg
    34321 aacaggcgtt gcgcgatgcg cgtctgaagc caagtcaaat cgacagtctg gtactggttg
    34381 gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac
    34441 cgtatcaaag ttacgacccg agcaccattg tcgcgctggg cgcagcaatc caggccgcct
    34501 gccgcttacg cagtgaagat attgaagagg tgatcctcac tgatatttgc ccttactcgt
    34561 tgggcgttga agttaaccgc cagggcattt ccggcatttt ctcgccaatt attgaacgaa
    34621 acaccacagt accggtgtcg cgtgtagaaa cttactcgac catgcacccg gaacaggatt
    34681 ctattacggt taacgtctat cagggagaaa accacaaagt taaaaataac attctggttg
    34741 aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat attcgcttta
    34801 gttatgatat caacggcttg cttgaagttg acgtgcttct ggaagatggc agcgttaagt
    34861 ccagagtcat taatcatagc ccggtaacat tgagcgcgca gcagattgaa gagagtcgga
    34921 cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaaccgc acctttaagg
    34981 ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag attggccggg
    35041 tgatcaccga ttttgatgcg gcgttgcagt caaacgatat ggcccgcgtc gatgaggtca
    35101 ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg ccggatgcgg
    35161 cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac aacatcgcga
    35221 ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata atgctggtaa
    35281 tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt gttggctcat
    35341 cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg gtgataatgg
    35401 cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc agcatctctt
    35461 gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc agtaacggtg
    35521 aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct ttgcgtttga
    35581 agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta tgtaaaattt
    35641 ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc ggttgacctt
    35701 gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa taatccgggc
    35761 aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg taactttccc
    35821 ggtacgcgca gagatcggca agcaggcttt ccccctcgcc gcagtacaac ctttgagcac
    35881 acaagtaata tcgggcatct tcgctttctg gttcttccat ttgccactgt ttggctacag
    35941 gaatgagttc cgccatacct gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta
    36001 actttgcatc atcaggaatg aaccacggac cttccatcgc caaaaacgca gtgacgtagt
    36061 caggatggta acggcagcaa cgttccagcg cgaagtaata ctcaacagtc tggttctgca
    36121 acgcaacggg atattccaga aggctaagga aatcgaaaca atcaccagcc tgaatggcct
    36181 caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac gaatgccagt
    36241 tcaaacgctc tgccagcaaa gaagcacaat ttaatgaaag atatcgcgct tctatagcga
    36301 ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta tttaattgct
    36361 gaataaattt ctgccaggcg gagggctgaa aacgatcact ttccgaatcc agtaatgtcc
    36421 tgaatgcacg cagtatttca tgttcagcgg cggcatcttt ttcttcatca tctgcttcct
    36481 cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat gctttaaacc
    36541 cctgcggatc ggtttcggga tgacataacg gtaagcgagc aagataagcc tggcggataa
    36601 tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc ataggtcgct
    36661 cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc agggaagcgt
    36721 ggcatagaaa cgatcgcttc ccgctgccag ctcttcgaac atccgactcc cggcgctatc
    36781 gtatttgtac agggttagcg cctcgcgtag cgaagtgttg tcttcgcact gagaagattg
    36841 ccagtagaga cgtcctaacg aataggcgga aaaatagtgc atccagctgt gatagtaatg
    36901 gtgagctcgt acatagacgc gcgattgcag ccataaactc tcttcttccg tcagccagtt
    36961 gttgagcaca ccgacccgtg acagaaaccc cattcgaaca taatcccagg ctttgattcc
    37021 cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca accctctctc
    37081 cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgatgcc atgtcagata
    37141 taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag tctgaattaa
    37201 atcatcacga ttatttattc cccaggatga tgagagatcg aattctttta tcccggtata
    37261 catttttggc gagtcatgcg cgggtttgca accctgatta agtatttcca tgggcgcaga
    37321 aagcgcaaaa agaagatatt gttcttcctt attcatttgt taataat
//
LOCUS       DAPHJE010000040        36161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPHJE010000040 DAPHJE010000000
VERSION     DAPHJE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.040"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            157..1129
                     /locus_tag="RH222_001982"
                     /pseudo
     CDS             157..1129
                     /locus_tag="RH222_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISEc18 family transposase"
     gene            1185..2641
                     /locus_tag="RH222_001983"
                     /pseudo
     CDS             1185..2641
                     /locus_tag="RH222_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478260.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(2906..4168)
                     /locus_tag="RH222_001984"
     CDS             complement(2906..4168)
                     /locus_tag="RH222_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV3797006.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHGDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTSVKRQHRPALDLSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(4366..4441)
                     /locus_tag="RH222_001985"
     tRNA            complement(4366..4441)
                     /locus_tag="RH222_001985"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4406..4408),aa:Phe,seq:gaa)
     gene            complement(4547..5254)
                     /locus_tag="RH222_001986"
     CDS             complement(4547..5254)
                     /locus_tag="RH222_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV3797007.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            5652..7787
                     /gene="speC"
                     /locus_tag="RH222_001987"
     CDS             5652..7787
                     /gene="speC"
                     /locus_tag="RH222_001987"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3797008.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTMRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(7837..9093)
                     /gene="nupG"
                     /locus_tag="RH222_001988"
     CDS             complement(7837..9093)
                     /gene="nupG"
                     /locus_tag="RH222_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV3797009.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(9295..10374)
                     /gene="mltC"
                     /locus_tag="RH222_001989"
     CDS             complement(9295..10374)
                     /gene="mltC"
                     /locus_tag="RH222_001989"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV3797010.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(10439..10714)
                     /locus_tag="RH222_001990"
     CDS             complement(10439..10714)
                     /locus_tag="RH222_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV3797011.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(10742..11794)
                     /gene="mutY"
                     /locus_tag="RH222_001991"
     CDS             complement(10742..11794)
                     /gene="mutY"
                     /locus_tag="RH222_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV3797012.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            11955..12674
                     /gene="trmB"
                     /locus_tag="RH222_001992"
     CDS             11955..12674
                     /gene="trmB"
                     /locus_tag="RH222_001992"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV3797013.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            12674..13000
                     /locus_tag="RH222_001993"
     CDS             12674..13000
                     /locus_tag="RH222_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV3797014.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            13184..13903
                     /locus_tag="RH222_001994"
     CDS             13184..13903
                     /locus_tag="RH222_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV3797015.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            14079..15125
                     /gene="ansB"
                     /locus_tag="RH222_001995"
     CDS             14079..15125
                     /gene="ansB"
                     /locus_tag="RH222_001995"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV3797016.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            15242..16249
                     /locus_tag="RH222_001996"
     CDS             15242..16249
                     /locus_tag="RH222_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV3797017.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(16404..17540)
                     /gene="hemW"
                     /locus_tag="RH222_001997"
     CDS             complement(16404..17540)
                     /gene="hemW"
                     /locus_tag="RH222_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV3797018.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(17533..18126)
                     /locus_tag="RH222_001998"
     CDS             complement(17533..18126)
                     /locus_tag="RH222_001998"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV3797019.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(18134..18424)
                     /locus_tag="RH222_001999"
     CDS             complement(18134..18424)
                     /locus_tag="RH222_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV3797020.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(18421..18987)
                     /gene="yggT"
                     /locus_tag="RH222_002000"
     CDS             complement(18421..18987)
                     /gene="yggT"
                     /locus_tag="RH222_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV3797021.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(19005..19709)
                     /gene="yggS"
                     /locus_tag="RH222_002001"
     CDS             complement(19005..19709)
                     /gene="yggS"
                     /locus_tag="RH222_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV3797022.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            19727..20707
                     /locus_tag="RH222_002002"
     CDS             19727..20707
                     /locus_tag="RH222_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV3797023.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(20870..21286)
                     /gene="ruvX"
                     /locus_tag="RH222_002003"
     CDS             complement(20870..21286)
                     /gene="ruvX"
                     /locus_tag="RH222_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV3797024.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(21286..21849)
                     /locus_tag="RH222_002004"
     CDS             complement(21286..21849)
                     /locus_tag="RH222_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV3797025.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(21958..22908)
                     /gene="gshB"
                     /locus_tag="RH222_002005"
     CDS             complement(21958..22908)
                     /gene="gshB"
                     /locus_tag="RH222_002005"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV3797026.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(22921..23652)
                     /gene="rsmE"
                     /locus_tag="RH222_002006"
     CDS             complement(22921..23652)
                     /gene="rsmE"
                     /locus_tag="RH222_002006"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV3797027.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(23732..24439)
                     /gene="endA"
                     /locus_tag="RH222_002007"
     CDS             complement(23732..24439)
                     /gene="endA"
                     /locus_tag="RH222_002007"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV3797028.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(24534..25031)
                     /locus_tag="RH222_002008"
     CDS             complement(24534..25031)
                     /locus_tag="RH222_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV3797029.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(25108..26502)
                     /gene="galP"
                     /locus_tag="RH222_002009"
     CDS             complement(25108..26502)
                     /gene="galP"
                     /locus_tag="RH222_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV3797030.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(26938..28092)
                     /gene="metK"
                     /locus_tag="RH222_002010"
     CDS             complement(26938..28092)
                     /gene="metK"
                     /locus_tag="RH222_002010"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV3797031.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(28396..28611)
                     /locus_tag="RH222_002011"
     CDS             complement(28396..28611)
                     /locus_tag="RH222_002011"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797032.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            28747..28878
                     /gene="yqgB"
                     /locus_tag="RH222_002012"
     CDS             28747..28878
                     /gene="yqgB"
                     /locus_tag="RH222_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV3797033.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            28887..30863
                     /gene="speA"
                     /locus_tag="RH222_002013"
     CDS             28887..30863
                     /gene="speA"
                     /locus_tag="RH222_002013"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV3797034.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            31009..31740
                     /locus_tag="RH222_002014"
     CDS             31009..31740
                     /locus_tag="RH222_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797035.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            31876..32796
                     /gene="speB"
                     /locus_tag="RH222_002015"
     CDS             31876..32796
                     /gene="speB"
                     /locus_tag="RH222_002015"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV3797036.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(33002..33760)
                     /gene="loiP"
                     /locus_tag="RH222_002016"
                     /gene_synonym="yggG"
     CDS             complement(33002..33760)
                     /gene="loiP"
                     /locus_tag="RH222_002016"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV3797037.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            34038..36029
                     /gene="tkt"
                     /locus_tag="RH222_002017"
     CDS             34038..36029
                     /gene="tkt"
                     /locus_tag="RH222_002017"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV3797038.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
BASE COUNT         8856 a         9085 c         9411 g         8809 t
ORIGIN      
        1 atttatagat tttgttaatg ttatctgttt tcaatctaac aaccacgaat atagctaatt
       61 ataggatgat tactagtttt actaccctat caaccaaacg ttcaccggac attagatgtg
      121 tgattcatag acatcgtttc actataatgc cttactatga acagggaaat tcagcagcgg
      181 ctacagtggg taaaactcta tgaaacgtct agtgacgcgg ggttcgtatg ccgtcgctgc
      241 ggtatttccc accccaccat tgcgtaaatg gtggcgcaga tatctggctc agggcatcgc
      301 tggtctggaa agccatagcc gtcgccctaa acattcacca tcaacgaaaa ccggcgctgg
      361 cgaagtcgct ctgattctgg agttacgttc cctgcgaaat ttgggtgcaa ggaggattca
      421 gagcgagtta aaacgacttc atgcaatctc actggcagtg gcaacgatcc acaaagttct
      481 ttgccagaat aaggttaaac ctgtcgttaa attccggcgt aaagcagatt ttattcgcta
      541 cgaacgccct gttcccggtg atcgggtcca gatggatacc tgtaaaattg gccccggttt
      601 gtatcagtac acatctgttg atgactgcac tcgctccaga gtcctgagac tctactctcg
      661 tcgcacggct gcaaacacac tggattttat cgattgtgtt attgaagaaa tgcctttccc
      721 gatacagcgc ttacagacag accggggaag agagtttttt gccgttaagg tgcaggagaa
      781 attgaaagaa tacagcatca agtttcgtcc aaataagcca gcctcacctc atcttaatgg
      841 caaagttgaa cgttctcaaa agacagacaa agccgagttt tacgctaccg tagacctctc
      901 aactgatgac ctgaaagaac tgctggcaga atggcagcat tactataact gggaacgccc
      961 tcatagcgct cacaacggta aaactccgat ggagcgctac ttcgaacttg cagagcaaac
     1021 accatactct gatgccgttc acgcaagcta tcagcccaat gaagagcata tacaggaaca
     1081 gaattacaag cttgaacttg aactgagaaa attgaaacga tgtctatgaa tcacacagat
     1141 gaatatcaga aaactgtttt gtccgggccc ccccccgggt tttattgttt ttattctttt
     1201 ttgttgtttc tgtcataacc acaattgcat acggatacac tgagaagaat gccaccggga
     1261 atgtgttgct gctgttcctt cttctgcttc ttgcacacag aaataccctc acatccatta
     1321 cagctctctg ttatttctgt tctgttgtgc actgtatgcg cctgccggta tgacgtacgg
     1381 taaaatcaac aacagtctta ttgtcgcgtt gttgcagacc acaactgatg aggcagcgga
     1441 gtttaccggg atgattcctg tttatcattt tctggtcagt gccgcgattc tggtattcat
     1501 ggtgattttc tggcgaacac accaccgcgg tcaccgtaac tggctggcac tgctgctatt
     1561 cgtattatgc tcagtaaaca gttggccgtt gcagatagtt aaaggaactg ttgtggggac
     1621 aactgacaca ttgcgtgaaa tgcagcatta taaacaactg agtcagcacg gggctgacaa
     1681 ctggaaaatc ctgccgggaa cacctttata tgacacgatt gttattgtca ctggtgagag
     1741 tgtgagcagg gattatatgt cggtatatgg ttatcccgtg ccaaccacac cgtggctgaa
     1801 tacggctccc ggtttattta ttgacggcta tacatcgaca gcagccagca ccgtgccctc
     1861 cctgagccgg acactgattt atgactatga gcagaaccct gattccggca acaacgtggt
     1921 ggcgctggca gcaaaagcag gatacagcac atgatggata tccaatcagg gaaaactggg
     1981 agagcatgac acacgcatct ctgttattgc ttctgatgca gagcataccg ttttcctcaa
     2041 gaaaggcagc tttgcttccc gtaaaacaga tggcatgcag ttgttacagg aaacagaacg
     2101 tgcgctggcg gatacatcct ctccgaaggt cattttcctg cacatgatgg gttctcatcc
     2161 gaatccgtgt gaccggctta actcctggcc gaataattac caggagcagt tcccacggaa
     2221 gattgcctgt tacctcgcca gcatcagtaa actggataac tttctcgggc agcttgatgg
     2281 tatccttcgc tggcattccc gtcattttgc catgctttac ttttctgacc atggactgtc
     2341 agtcagcgac agtactaatc ctgttcatca tgatggccat gtgcaggctg gctacagtgt
     2401 tcccctgatt attaccgcca gtgacatcac atctcaccag tccgtcagca gaaaaatcag
     2461 tgcccgtcat ttcgcaggca tttttcagtg gctgactggt attcgtactg aaaatatacc
     2521 gccattcaac ccgctgataa atgaagataa tgaacctgtt atggttttta acggagagag
     2581 aatagttcct gcggacggcc ttaagtcaca gcctgtgatt cttccgggca agggaaaata
     2641 agagtaatgt tgagcaccat gttctgtacg atgaattttc cactgtttat atcgattatt
     2701 ttgctaataa ccagagttga gcacccgaaa atacctgaag aggaattacc cgctaccaga
     2761 aggttcagat atccctgatg tcggctataa aaattttgat tacatgacgt aatgaaagtg
     2821 gctctgtcaa ttgccttgtt tattcattaa tctcaaagaa aaaggtattg aaaataattc
     2881 ccgggctgcc agcccgggat gattactatt ttaatggatt gttaatcttt gcatattcaa
     2941 acgggctgat acattgttct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     3001 gtcgccgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     3061 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     3121 cactgcacgc catcgcccgg aatccatggc cacagacttc ctgggttgtg tcatacccca
     3181 tcaccctcag ggctttattt acagtatttt cactgattgg tttacgcggg tcatgtgctc
     3241 ccgtaaagat cagaccattt tcaccagccc atgtctgtag ttctgccagt atggctacag
     3301 cctgtttgct gagtggcaca agatgtggtg ttcgcatctt ggagccccgg tgtgagaatt
     3361 ttacgcctgg cagaggtttg cgttcggctg gaatagtcca catggcattg tcaatatcaa
     3421 tttcagacca acgggcatat ctgagttcac tggagcgaat aaaaaccagt aaattcagca
     3481 taactgccag tcgggtgaca ggctgacctt tataactgtc aatgcgcgac agaagttcag
     3541 gcaggcgtga aagatcaaga gcggggcgat gctgtcgttt tacagaggtc aatgcgccag
     3601 ccatatccag agcaggatta tatctgatga tgccagactg tacggcataa cgcattacag
     3661 cagatattct ttgctgcaga cgactggcga tttcataaat ttgttttgct tctgcagcac
     3721 gaacaggaat aagcaggtcc ggggtattga gtgttgtgat atcccggttg ccgataaagg
     3781 ggaaaacgtg tgtctcgagg cttttgagta ctttatcgcc gtgtgattgt gcccattgtt
     3841 tattactggc atgccagcgt cgtgcaatat gttcaaaaga gagctgtaaa tcagggacta
     3901 cttttttaac tcttttctgt tcgcaagggt caatcccctc tgaaatcagt tttcgggcct
     3961 catcccgttt tgtacgagct tcggagagag aaacttccgg atacacacca agtgccaggg
     4021 ttttctcctt acccagaata cgataacgga gacgccagta acgggaacca ttaggatgaa
     4081 ccagcaggaa catgccggcc ccgtcagtga gtttataagc cttgtcagtg ggctttgcag
     4141 cccggatttt tgcgtcagtc agtgccatag ttcagccttc tgaatgcgtt aaagagggta
     4201 caaattctgt cgaactgggg tatacccgca attataccca cacaatgagg ttgatgtggg
     4261 gagaatctgg ttgagttcgg tagaattgat ttggaggaag aacacttaaa tcatggtgtt
     4321 ctgaaacgaa aaacggacct ccgtggaggt ccgttgatat gaatttggtg cccggactcg
     4381 gaatcgaacc aaggacacgg ggattttcaa tcccctgctc taccgactga gctatccggg
     4441 caacggggcg cattaaacct gattcgcctc gtctcgtcaa tcaaatttct tcaatttact
     4501 gcagactgca caatctatca tcactttgcc gctattgcac gcattgtcag gcaaaccagg
     4561 cggtccaggc ggcggaaagc ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg
     4621 ggaacatttt aatgccacag atgcgtaatc cggttgccag cagcaataaa ccgcctacgg
     4681 cgctgaagtc tgccatcatc gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg
     4741 ccagcgttaa ctggatgatc agtaatggga tactaattac cgacaccgca atacccagcg
     4801 agcaggcgaa gatcatcgcc gtaaagaaat caagaaatga cttggcgatt aaaatactcg
     4861 gatcgccggt catcccttcg ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca
     4921 ggacaataat cgcgacataa ttctgaataa aagattcatg cgctggcttc ttacgtgagt
     4981 gacgaaacag attttgtgct ttggtgactg ctgtattgac acctttctcc agcaaacaaa
     5041 tttcgccaat tagagcgcca agtagcgtag ctaaaaccat cgctggaagg ttggcacatt
     5101 tcaccaccag taaaatacca atccccagcg atgccagacc aaaaatagat gtcatggaga
     5161 cgcggatacg ttccggtaag cgttggctga gcagtgcgcc gagaacgcca cccagtaata
     5221 ctgcactagc gttgataaaa gggccgatga ccacaagagt tcctgttaac tgtattgttt
     5281 gattttctta ttatgaccgt ttggtgccgt cgatggcttg tttgttcgca aagtcctggc
     5341 ttgcacgctt tagcgaaagg tgccatgatt gcgcgaattt tctcctctct gtacggagtt
     5401 tgcccgatgc acgccacctc cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt
     5461 tccctttcta tgttactgct catgcggtga agttaacgca cgctcactgc aggacaacag
     5521 taaaatcaga gcgtttctgc ttttactgat gtctggcggt cggagctggt gaccagtttg
     5581 acccatatct catggggcag ggttttccac cttgtccggt attcttactt ccccgaaacg
     5641 ggtttgcgct tatgaaatca atgaatattg ccgccagtag tgatctggta tcccgacttt
     5701 cttctcatcg tcgcgtggtg gcgttgggag atactgattt tacggacgtc gcggcagtcg
     5761 tcattaccgc tgcggatagt cgcagtggca ttcttgcgtt gcttaagcgc accggttttc
     5821 atctaccggt gtttttgtat tccgaacatg ctgttgaatt acctgcgggc gttacggcgg
     5881 taatcaacgg caacgagcag caatggctgg agctggaatc cgcagcctgt cagtatgaag
     5941 agaatttgct gccaccgttt tatgacacgc tgacgcagta cgttgagatg ggcaacagca
     6001 cctttgcttg ccctggacat caacatggtg cgttttttaa aaagcatcct gccggacgcc
     6061 atttttacga tttctttggt gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa
     6121 aattgggcga tctgcttatt catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca
     6181 aagtctttca tgccgataaa acctattttg tgctgaacgg cacatcggct gcgaataaag
     6241 tggtgacgaa tgcgctgtta acgcgtggcg atctggtgct cttcgaccgt aacaaccata
     6301 agtcgaatca tcacggcgcg ctgattcagg cgggggcgac gccggtctat ctggaagctt
     6361 cacgcaaccc gtttggtttc attggcggta ttgatgcgca ctgttttaat gaagagtatc
     6421 tgcgccagca aattcgcgac gttgcgccag aaaaagctga gttgccacgc ccgtttcgcc
     6481 tggcgattat tcagctggga acctatgacg gcactgtcta taacgcccgt caggtgatcg
     6541 ataccgttgg gcatctgtgt gattacattc tgtttgattc cgcgtgggtc ggttatgaac
     6601 aatttatccc gatgatggcg gatagctcgc cgctgctgtt agaacttaac gaaaacgatc
     6661 cggggatctt tgtgactcag tcggtgcaca aacagcaggc gggattctca cagacgtcgc
     6721 agatccataa aaaagataac catatccgcg gacaggcgcg tttttgcccg cataagcggt
     6781 tgaataatgc ctttatgctc catgcttcta ccagcccgtt ctacccgctg tttgccgcgc
     6841 tggacgttaa cgctaaaatt catgaaggag agagtgggcg tcggctgtgg gctgagtgcg
     6901 ttgagttggg tattgagtcg cgcaaggcta ttcttgcgcg ctgtaagctg ttccgcccgt
     6961 ttatcccgcc cgttgttgat ggcaaattgt ggcaggatta tccgacatct gtgttagcca
     7021 gcgaccgccg ttttttcagt tttgagccgg gggcgaagtg gcacggcttt gaaggatatg
     7081 ccgcggatca gtattttgtt gatccgtgca agctgttact cactacacca ggtatcaatg
     7141 ccgaaactgg cgaatatagc gactttggcg ttccggcgac gattctggcg cactatctgc
     7201 gtgagaacgg cattgtgccg gagaagtgcg atctcaactc cattctgttc ttattaactc
     7261 cggcggaaag ccacgagaag ctggcacaac tggtggcgat gctggcgcaa tttgaacagc
     7321 atattgagga tgactcgccg ctggctgagg tgttgccgag cgtttataac aaatatccgg
     7381 tgcgctatcg cgactacacc atgcgccagt tgtgtcagga gatgcacgat ctgtatgtca
     7441 gtttcgacgt caaagaccta caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg
     7501 tgatgaaccc ccaggatgcg catagcgctt atattcgcgg tgaagtggag ttggtgcgga
     7561 ttcgtgatgc cgaagggcga attgcggcag aaggggcgtt gccttatcca cctggcgtgc
     7621 tttgcgtggt acccggggaa gtctggggtg gggcggttca acgttatttc cttgcactgg
     7681 aagaaggggt gaatttgttg ccgggatttt cgccggagct gcaaggtgtt tatagcgaaa
     7741 ccgatgcgga tggcatgaaa cggttgtacg gttatgtgtt gaagtaagaa taaaaaaaac
     7801 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     7861 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     7921 ggagtaacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     7981 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     8041 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     8101 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     8161 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     8221 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     8281 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     8341 ctgcacaata aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     8401 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga taaagaagat
     8461 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     8521 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     8581 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     8641 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     8701 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     8761 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcctccgg
     8821 tgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
     8881 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
     8941 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
     9001 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
     9061 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
     9121 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
     9181 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
     9241 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
     9301 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
     9361 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
     9421 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
     9481 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
     9541 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
     9601 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatg
     9661 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatatg ggttaaagga
     9721 agattcggtc tgcataatcg ccagaatcag cgactcatca acgccatatt tccgtgacgc
     9781 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
     9841 aatggtaacg ctgtagatga tacgcagccc gttgctgcgg ctcttcagac ggtttttcag
     9901 cagataatcc gcgaagttgc tcgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
     9961 caccacctga ccgtaaagga aaggttcttt cgaaatcgta atatcatcaa cgtcggaata
    10021 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    10081 atgcgcggca ggttctgtcc cggcgatagt ttcgatagta atcgtaccgt catcgaagtt
    10141 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    10201 caccacctct ttgaatcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    10261 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    10321 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    10381 gggtgcgtgt tgtgtttgtt tttatgcaag ttgccggagg cgtgctccgg cactgttttt
    10441 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    10501 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    10561 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    10621 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    10681 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    10741 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    10801 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    10861 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    10921 gccgaaacgc ggtcagctgc gtcaggttat cggcagcaat ctgccgttgc gccagccact
    10981 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    11041 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    11101 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    11161 cggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggtttc gagcgcgtac
    11221 aaatcatcgc acccaaatcc atcatcgcct gattaaaccg ttccacgcca accgcgggcg
    11281 tcacctgctc gctcaaactc cataatttat tctcgacctc ttttttccct ggccagccgc
    11341 ttacagcata gcagcgcgcc agcacccgtt tgacgttacc gtcgagaatc ggaaagtgct
    11401 tacccagaga aagcgagaga atcgcacctg cggtggaacg cccgacgccc ggtaacgccg
    11461 cgacttcttc aaaggtttcc gggaatttac cgccgtgtaa ggtcgccact tgttgtgccg
    11521 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    11581 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gcgcgccata aagcgttcaa
    11641 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    11701 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    11761 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgaattcact gttgttgggg
    11821 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    11881 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    11941 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    12001 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    12061 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagcgaagat atgctggatt
    12121 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    12181 catcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    12241 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    12301 gagtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    12361 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    12421 tcgtacaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggggtattcc
    12481 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    12541 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    12601 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    12661 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    12721 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    12781 acagattgat aaaaccgttg atgattttat taacgaggtt atcgaaccga acaaactggc
    12841 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    12901 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagcgca aactggatga
    12961 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    13021 aaatgcaaaa ctgcctgatg cgctacgctt atcaggcctg gaaagatgca cgatcgagta
    13081 ggcgggataa ggtgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    13141 ggggccagca aatgctggcc tgattttttc ttgagggaag actatgatgc gcaaaatgct
    13201 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    13261 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    13321 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    13381 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgcagca cgctgccgtg
    13441 gattgatgaa ggcgcgaaaa gccgcgtcga aaaagcccgt attgcgctgg ataaaattat
    13501 cgttcaggag atgggcgaaa gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    13561 gctgaaagag cagatgaacc gcattatcga aacgcgcagc gatggcctga cgtttcacta
    13621 taaagccatt gatcaggttc gcgccgaagg ccagcaatta gtgaatcagg caatgggtgg
    13681 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    13741 cccattacag aatgtgctgg gaagcctggg ggggctgcaa tcctcaatcc aaaccgagtg
    13801 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    13861 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    13921 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    13981 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat acagagctaa
    14041 gggataatgc gtagcgttca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    14101 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    14161 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    14221 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaactg aaggacattg
    14281 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    14341 ggctgacact ggcgaaaaaa attaacaccg actgcgataa gaccgacggc ttcgtcatta
    14401 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    14461 acaaaccggt ggtgatggtc ggcgcaatgc gtccgtccac gtctatgagc gcagacggtc
    14521 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgcc aaccgtggcg
    14581 tgctggtagt gatgaatgac accgtgcttg atggccgtga cgtcaccaaa accaacacca
    14641 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggttacatt cacaacggta
    14701 agattgacta ccagcgtacc ccggcacgta agcataccag cgacacgcca ttcgatgtct
    14761 ctaagctgaa tgaactgccg aaagtcggca ttgtttataa ctacgctaac gcatccgatc
    14821 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    14881 acggcaacct gtataaatct gtgttcgaca cgctggcgac cgccgcgaaa aacggtactg
    14941 cagtcgtgcg ttcttcccgc gtaccgacgg gcgctaccac tcaggatgcc gaagtggatg
    15001 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgttctgc
    15061 tgcaactggc tctgacgcaa accaaagatc cgcagcagat ccagcagatc ttcaatcagt
    15121 actaatcacc tcgccccggt atcgtgccgg ggctttttca cttcagactt acgtccattg
    15181 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgtg aggtttttcg
    15241 ggtgaaaaag caatggattg ttggcacggc actgcttatg ttgatgactg gtaatgcctg
    15301 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    15361 cggcattctc aagcttgatg ctatcacctt aaaaaatctt gatgctaagg gtaatcaggc
    15421 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    15481 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    15541 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    15601 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    15661 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    15721 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    15781 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    15841 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    15901 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    15961 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    16021 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    16081 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    16141 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    16201 ctggaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaag tttgatatag
    16261 tgcacgattt gtagcaggat gcggcgttga ccccgcatcc ggcaatcaac gtctgatgcg
    16321 acgcttgcgc gtcttatcag acctacaacc gctgccacat gtcggctgga taaggcgttc
    16381 acgccgcatc cggcaataca agtttactca gccagaaaaa gctccagcag cgaatttaaa
    16441 aacagcttcc catgttccgt tatctgccag taatccgcac attcggtgag ataaccctgg
    16501 gcaatcgcct cgtctaactg tgggcgaatc acatcttcgc aaagcccggt atacgcacta
    16561 aactctgcgc gtggcgcggc ctccagcaag cgaaagcggt tcataaagaa ctcaaacggc
    16621 ttatctgcgg cttcgacatc acgctggctt tccagatacc ttccttgcat aaaaccacgc
    16681 ggatgacgcg ttttagtggt acgcagaatg cgcccatccg ggaaggtcac tttgccgtgc
    16741 gcgccgcagc caataccgat gtagtcgcca aagcgccagt agttgagatt gtgctggcac
    16801 tgataactgg gtttggcgta agcggaagtt tcatattgct gataacccgc tgcggttaat
    16861 aactgatgcc cctgttcgaa tatatcccac agcgcgtcgt cgtccggcag taccggcggg
    16921 cgtgaaccaa acagcgtatt aggttcgatg gtcagttgat accaggaaag atgcggcgga
    16981 ttcagttcaa tggcctggcg tagatcgcca agcgcctctt ccagtgattg atccggtagt
    17041 ccatgcatca aatcgaggtt aaagctacgc agccccagcc cgctcgccag ctttgccgcg
    17101 cgtttcgctt cttgcgggcc atgaatacgc ccaagtcgtt tcagcttttc ttcgctaaaa
    17161 ctctgcacac caatagagat gcggttcaca ccagcacgct gataatcgac aaagcgatcg
    17221 gcttctaccg taccagggtt cgcttccata gtaatttctg catccgctgc cagcggcaaa
    17281 cgcgcacgca cgccgtccag cagcgtttgc atcgccgggc cggaaagcag gctcggcgta
    17341 ccaccgccaa taaagattgt ctttacttca cggccctgag cgtaagccac atcattgtcc
    17401 agatcgcaaa gcagatgctg aacgtaatcg tcgtgcggca cttctccttt caacgcatga
    17461 gagttgaaat cgcagtacgg gcatttctgc acgcaccacg ggatgtgaat gtagagactc
    17521 agcggcggta atttaaccat tacgtaaagc gtccaacagc agtttcaatg cctgaccacg
    17581 gtgggaaatg gcgctctttt cttcgcgggt cagttcggca gcggttttcc cttcggaagg
    17641 tacgaagaag attggatcat aaccaaagcc accagtgccc gctggttcac gagtaatcac
    17701 gcccggccag ctaccgtggc acaccagcgg agtcggatct tccgcgtgac gcagatatac
    17761 cagcacgcag tggaaacgcg cctgacgttg gtcgtccggt acgtctttca gtgtttccag
    17821 tagtttttgc agattcttta gatcggtcgc gtcttcaccg gaataacgcg cggagtaaat
    17881 gcccggcgcg ccgccaagca catctaccgc cagaccagag tcgtcggcaa ttgccggtaa
    17941 accggtcact tttgccgcat ggcgcgcttt cagaatcgcg ttttcgataa aggtcaggcc
    18001 ggtttcttca gcggaatcta cgccgaggtc tgtttgggcc acgatatcaa gaccgaagtc
    18061 gctaagcagc gacgccagct cacgcacttt accggcattg ccggttgcga ggacaacttt
    18121 ttgcatagga tacctaatta attaacgccg cgatttctgg cgggatttgt tgcggattaa
    18181 tgattttaat ttgtttgtgg cggccaagtt cgcctttttc aatcaccacc tggcttttgg
    18241 caacccggaa ttgcttaccg agaaacttca ccagatgact gttggcctgg ccgtcaaccg
    18301 gcggcgcggt aatggcgact ttaacttcgt cgccatgtaa accgacaata gaatcacggc
    18361 tggctttcgg ctgaatatag agccgtaaaa ccagaccgtc atcattaact gttacggcac
    18421 tcataacgcc atccacagcc ccggcagcag catgtttccg gttgcctgta atacttctgc
    18481 gacacccata ttgatgacat acagcagcag aacgaggatc atcggcgaga aatcaatccc
    18541 acccattgcc ggtagcaggc ggcgaatcgg gcgcagcagc ggatcggcca gctgaatcag
    18601 cacgtattca atcggactac gaccctggct tacccagctc ataatcgcca tcaccagcag
    18661 gacccagaaa atcagcaagc cgatggtttt cagcagaatc agtaaaccgg caatccagat
    18721 gattggcagg aaggtcacca ctttaaacag cacaatggct ttgataaaac tgagaatata
    18781 ggcaaccagc agcgaggcgc tgtcaatggg acccattgcc ggaataacgc ggcgcagtgg
    18841 cccgataatt ggctgcgtta ccttcactac aaactgtgag aaggggttgt aaaaatcaca
    18901 atgagcccac tgcatccaga tgcgtaataa cagcaccatg gtatacagct caatgaccgt
    18961 tgaaagcagg aaagtcaacg tattcatggc gttcctttaa ttccttattt tttagagtaa
    19021 tcacgcgcac caaaaattgc agtgccgata cgaaccatcg tgctacctgc cgcaatggcg
    19081 gcttccatat cgtccgacat tcccagagag agcgtgtcga tatgcgggta gcgcgttttc
    19141 agtccggcaa atgctacagc catttggcgt gcaacttcaa actgccttac atattctgac
    19201 tcaggcgcag ggattgccat cagcccgcgc agacgtaaac gcggtagttc agcgaccgcc
    19261 gctgccagct cgtccagttc tgccagttga atcccggact tactgttttc atcactaata
    19321 ttaatttgaa tcagtacgtt aagaggggga agttctgccg ggcgctgatc gttgaggcgg
    19381 gtagcaatgc gcaaacggtc gatggtatga caccagtcga aatgctctgc caccaggcgg
    19441 cttttattag actgcaacgg gccaataaaa tgccattcta atcctgttac gcccagttcc
    19501 tgaaagtggc gaattttatc taccccttcc tgaacgtagt tttcaccaaa ttgacgctgc
    19561 ccggcatcaa tggcttctgc gatggcgctc gcaggttttg ttttactgac tgcaagcagc
    19621 gtaatttctt ctggagaacg gccgcaacgc gttgcagccg ctgagatttt gtcccggacc
    19681 tgtgccaggt tatgcgcaat atcgttcatt ttccgaggat cttagtatga atatggaaga
    19741 aattgtggcc cttagtgtaa agcataacgt ctcggatcta cacctgtgca gcgcctggcc
    19801 cgcacgatgg cgcattcgtg gcagaatgga agctgcgccg tttgatgcgc cggacgtcga
    19861 agagctactg cgggagtggc tggatgacga tcagcgggca atattgctgg aaaatggcca
    19921 gctggatttt gccgtgtcgc tggcggaaaa ccagcggttg cgtggcagtg cgttcgcgca
    19981 acggcaaggt atttctctgg cattacggtt gttaccttcg cactgtccac agctcgaaca
    20041 gcttggtgcg ccaccggtat tgccggaatt actcaagagc gagaatggcc tgattctggt
    20101 gacgggggcg acggggagcg gcaaatctac cacgctggcg gcgatggttg gctatcttaa
    20161 tcaacatgcc gatgcgcata ttctgacgct ggaagatcct gtggaatatc tctatgccag
    20221 ccagcgatgt ttgatccagc agcgggaaat tggtttgcac tgtatgactt tcgcatcggg
    20281 gttgcgggct gcattgcggg aagatcctga tgtgattttg ctcggagagc tacgtgacag
    20341 tgagacaatc cgtctggcgc taacggcggc agaaaccggg catctggtgc tggcaacctt
    20401 acatacgcgt ggtgccgcgc aggcagttga gcgactggtg gattcatttc cggcgcagga
    20461 aaaagatccc gtacgtaatc aactggcagg tagtttacgg gccgtgttgt cacaaaagct
    20521 ggaagtggat aaacaggaag gatgcgtggc gctgtttgaa ttattgatta acactcccgc
    20581 ggtggggaat ttgattcgcg aagggaaaac ccaccagtta ccgcatgtta ttcaaaccgg
    20641 gcagcaggtg gggatgataa cgtttcagca gagttttcag cagcgagtgg gggaagggcg
    20701 tttgtgacgt tgccatgccc gatgtgcaag cttatcgggc ctacaaaggc aataaacgtc
    20761 tgatgcgacg cttgcgcgtc ttatcagacc tacaaccgct gccacatgta ggccggataa
    20821 ggcgtaaggc gcgatgatat tatacactcc agcaggcgtt taaatcgctt taatatccct
    20881 gctcgaaata gctttcgaga ataataaccg cagaggcaga gtcgactttg cctttattta
    20941 gcgcccgata gccgccctgt tcaaacagac cggaacgggc ttccacggtg ctaagacgtt
    21001 cgtcatggag ctttacttca acaccgaaac gaccatgaat acggttggca aatttacgcg
    21061 ctctggcagt caatggttgc tcggtgccgt ccatattcag cggcaaaccg acgatgattt
    21121 cgtccggctg ccactctttc agtaaacgct cgataatgtt ccagtccggc gtaccgtcct
    21181 gtgctttaat tgcaggcaaa gggcgagcgg tgccggtaat gcgttggccg accgctacac
    21241 caatactttt ggtaccgaag tcgaaggcga gtaaggttcc actcatcagg cgtgccctgc
    21301 cacaccaggc atggtgagaa tatccacacc aatcagtttt gccgcctcgc gccagcggtc
    21361 ggcaatcggt gttttgaaca gaatattcag atccgccggg gccgttagcc acgcgttatc
    21421 gagaatttct tgttccagtt gacctttttc ccaggaggca taacccagag ccaccaatac
    21481 gtcagacggt tgtttatcgg tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac
    21541 tgtgttgtct gaaatgcgaa tgctggaagc aaaattggag ggcggagtat gcaaaataaa
    21601 cccgcgatct tcagccagcg gaccgccgag cataaccggt ttatccagac ggattgattc
    21661 atcacgcggc tccggcgtga tcttcagctt ttccagaatc ccttcaattt tgagattttc
    21721 cagcggcttg ttgacgatga tccccattgc accattggta ttatgttcgc aaatgtagac
    21781 cacggaacga cggaaaatcg gatcctggag agcaggcatg gcaataagaa agtgatgctg
    21841 taaattcatt gtcagaggtt ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg
    21901 tggaaagaag ggcgctgtca ctcagagtct caacgagatc cttctcgcta aggtgggtta
    21961 ctgctgctgt aaacgtgctt cgatggcatc cattaacatt ccggtgatcg acaccggaaa
    22021 ctctgcttca atctcacgaa tacaggttgg gctggtgacg ttaatttcag tcagacggtc
    22081 gccgatgata tccagaccaa caaaaatcag ccctttttct ttcagcgtcg gcccgatctg
    22141 acgggcgatt ttccagtcac tttccgtcag cggacgaggt tcaccgcgtc caccggcagc
    22201 cagattgcca cgggtttcgc ccccctgcgg aatacgcgcc aggcagtacg gtaccggctc
    22261 gccatccacc accagcacgc gtttgtcgcc atctttaatg gctggcaggt aattttgcgc
    22321 catgcagtag cgagtgccat gctcagtcag ggtttcggca atcacgccga ggtttggatc
    22381 gccttctttc acgcggaaaa tcgacgcgcc gcccataccg tccagcggct taagaatgat
    22441 gtcgctgtgt ttctcccaga acgcttttag ctgcgcttta ttgcgcgtaa ccagcgtttc
    22501 tggcgttaag tcagagaacc aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg
    22561 cggcttgtta acgatcagcg tccctttctc ttcggcacgt tccagaatat aggtcgcgta
    22621 gataaactcg gtatcaaacg gcgggtcttt acgcatcagg atcacatcga gatcggccag
    22681 cggcagatcc tgttcaccga cgaatgaaaa ccactcttcg tagttctgct tcacgttcag
    22741 cgtgcgggta tgggcgcggg cttcgccatt gatcagatac agatcgccca tctccatata
    22801 gtgaagttcg taaccacgac gctgtgcttc cagcaacata gcgaaactgg aatctttctt
    22861 gatgttgatg tttgcgatgg ggtccatcac gatgccgagc ttgatcatta ttcttctccg
    22921 ttagcccaaa tcgccaaatc gtacttgtag cgcggtaatg gcggtgagcg cagttgtctc
    22981 tgtacgcaaa acgcgaggtc ccaacaggat atcagtaaat tgatagcggg cagtcatggc
    23041 aatttcatct gccgataaac cgccttccgg gccaatcagc aggcggacgc gttcaaccgg
    23101 taacggcaac gtattgatgc tgttactggc gcgcgggtga agattcagtt tcagtccttc
    23161 atcctgctct gcacaccagg cttccagatc catcgctggg cggatttccg gcacccggtt
    23221 acgaccacac tgctcacagg cagcaattgc aatcttctgc cactgctgga gcttcttgtt
    23281 cagacgttca ctatccagtt taacgccgca gcgctcagaa aaaagtggcg taatgaggct
    23341 tacaccgagt tcgatcgatt tctggatcgt aaattccatt ttttcaccac gcgacatcac
    23401 ctgaccgagg tgaatatgca gcggagattc gcgatcgtcg atcttgcctt ccagcacctt
    23461 cacttccacg ctttttttgc tggcgctggt aatttcggcg tcaaagacct ggttgctacc
    23521 gtcaaacaat tgcaacgcct gccccggccc catgcgcagt acgcgcccga tatggttggc
    23581 ggcatcttcg caaagcgcga tgtgagaatg gctggtcagt ggttcaggat gataaatgcg
    23641 ggggatacgc atagttaaaa atccgcgtcg tctccccacg cggttgtacg catggggtag
    23701 gggttaacaa aaagagtccc gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt
    23761 gcacatacgg gttatgattg ccttgcacct tcgcgatgcg ttcatcgcgc tcgcactccc
    23821 agtcggtaac cggatacatc ttgttccatg cgttgaacag ctgcgtttgc tggcgagaga
    23881 gtgtcaggtt gtattggtcg cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac
    23941 gcgctggtgg ttcggcagct ttttctttga aatcgacctt catggcgcat tgaccgtact
    24001 ggccttcgcc gccattccac tggctgtaca taaagttgcc gcgatcgcca ttcacctcac
    24061 cgactgacgg ctgcaggtta tgcatatcgc tttccatctt gcgatagacc ggatctttag
    24121 cgcagttttt acgtccaccg tcctgccagc actggcgctg gtgaccgaac tgccaggcgg
    24181 gaacgacgtg ttcccactct acgcggctgg ctcggttttc atttttgcgc acctgatagc
    24241 cgcacgattg cagatcaaca acgccttttt tgccctgcca gttaatttta catccgcaat
    24301 aaaacgtacc gggcgcgtca gcgtggactt ttaccgccgc ggctttcgcc tgagaaaaac
    24361 tattgatacc ttcggccaac gccgggccgg aaaatgctgc gctcagtacc accgcagcaa
    24421 tagacaaata acggtacatc acttactccg tgttaaaacg agccagcaac gtagcgaaag
    24481 ttgttttggt atgcaatcag ccagatcagg aaagttcctg atagttcaga tggttatttc
    24541 gcaaccagct gttcaccgca gtgaacacag cgatagacgg cctcgccacg aactacgcga
    24601 ttatggcggc gtacggtaag ctgatgctcc tggcacttgc agcggtaggg gaaggtgttg
    24661 cgacgcacgg attgcagttc gaactgatgc gtacgacggg cgggaacgcc cagcacgctt
    24721 tccatcatcc acttccactc tttgccatgt ggcgctacgc ggccgaaatg tttccatacc
    24781 agcaaatgtg ccagttcgtg cggtaccact tcttcaataa aagcttcact gttttccaac
    24841 agcaaaacgg gattgaggcg gatttcatag ctttccagcc aggctgttcc ggcggaggtt
    24901 ccgcgctggg tgtaagagag ttttggctcc gggtagttac gccctagctt caggttggcc
    24961 tgggcgagtt tttcccgcag gcggcgcata acggcctgct ggatggcgat agggagacgg
    25021 gatgttttca tagcggcaga ggatagagcg aagaaaagat gactgcaaga ggtggcttcc
    25081 tccgcgatgg gaggaagcct gaggagatta atcgtgagcg ccgatttcgc gcagtttacg
    25141 acctttcatc agattacgtt caatatgttc cagcgaaacg tgtttggttt ccggtaccag
    25201 ccacaatgtc agcaggataa acagtacgtt cagagccgca tacacccaga aggtgttggc
    25261 gttacccagc gtgttgagca tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca
    25321 gttggtggcg gtggagcagg tgatgccaaa atcgcggcct ttcagcggct gaatttcgga
    25381 gcacagtacc caaatcagcg gaccggcact catggcaaaa ccgacaataa acatcagcag
    25441 catggcgatg gcgaaatact gcgctgacgg agagtgaata ccgatatgca tcattgtacc
    25501 gagtacgccc atgccagcag ccatcaccag gaagcccagc gttagcgttg gtttacgtcc
    25561 ccagcggtca acaaggccga ttgcgataaa ggtggcaagt acgttggtca ggccgacaat
    25621 cacggtcccc cacatttgct cggtagtgtt ggtataaccc gccagttcga agattttcgg
    25681 cgcgtaatac atgatgacgt tcatcccggt gaattgctgc attacctgca acagtacgcc
    25741 aaggaacacc gcgcggcgga agttgctgtt ctctttaaac agcgcccagc cactctgttt
    25801 aacctgcaaa ctttcacgga tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg
    25861 caggcgtagc agcacgcgtt cggcatcaac aaaacggcgc ttggcggcaa accaacgtgg
    25921 gctgtctggc aggaagaaga caccaatcag cagcaaaatt gccgggatga taatcacacc
    25981 cagcatccag cgccatgcac cggtgtagct gaaggcggta tcagaaagat aagcaccgag
    26041 gatcccgata gtgatcatca actgatacat cgagatcata ctgccgcgaa ttttttccgg
    26101 cgcaatttct gacaggtaca gtggtgcggt ataagaggcc acacccaccg ccagccccag
    26161 cagaacgcgg gaaagaatca gtacttcaac gtttggcgca gccgcagaga acagcgaacc
    26221 ggcaacaaac aaaattgcgc cgatcatcag gctctttttg cgcccgagtt taaaggagag
    26281 ccagccgctg cccaccgcac cgactgccgc accgaacatc atggagctta cgacccattc
    26341 ttgcgtgtgc gaagtaatct ggaattcatc tgcaataaac ggcagtgcgc cagcaattac
    26401 accgatatcc aggccaaaga gtaatcccgc cagagcggca aggaagcaga cgaaaaaagt
    26461 cattgccttg tttgaccgcc cctgtttttt agcgtcaggc atgatgccct ccaatatggt
    26521 tattttttat tgtgaattaa gataggtgag tacgacgtaa aaagatgtga agcaaatcac
    26581 atcagtgtaa tcgcttacac taacatttat cttagttatg gtttaagtgc atgaaaataa
    26641 ttgagttata tcatcataga ttaaacgctg ttatctgcaa ttaagacttt actgaaaaga
    26701 aatgtaacaa ctgtgaaaac tgaataataa tatgaaaata ttacaaatat ttggctggtg
    26761 ttattggtgt aatcggtttc agtagtgatg ataatttgcg ataaaacgtg ttgaggaaga
    26821 atgatgttat tgtgcgggca gctttttgcc ggatgcggcg tgaatgccta tccaccgcgg
    26881 tgaggtaaaa gaaaaaggcc agcgcgaggc tggcctttga aagcaggtga agaaagatta
    26941 cttcagaccg gcagcatcgc gcagcagctg cgctttgtcg gttttttccc acgggaaatg
    27001 ttcacgacca aagtgaccgt atgctgcggt ttctttgtag atcgggtgca gcagatccag
    27061 catctgaatc agaccgtatg ggcgcaggtc gaagaactca cgtaccagca gagtcagttg
    27121 ttcagaaggc actttctcgg taccgaaagt ttctaccatg atggaggtcg gttcagccac
    27181 gccgattgcg taggaaacct gaatttcaca acgatcggcc aggccagcag caacgatgtt
    27241 ttttgcaaca taacgtgctg cgtacgcagc ggaacggtcc acttttgatg gatctttacc
    27301 agagaatgct ccgccaccgt gacgtgccat gccgccgtag gtatcaacga taattttacg
    27361 accagtcaga ccgcagtcac ccatcgggcc accgataaca aaacgaccgg tcgggttgat
    27421 gaagaatttg gtggcagaag tcagccattc agcgggcaga atcggcttga tgatctcttc
    27481 cattaccgct tcttgcagcg atttctggtc gatctcttca gagtgctgag tggaaagcac
    27541 gacagcatcg ataccaacga ttttgccgtc gtcatactgg aaagtcacct ggcttttcgc
    27601 gtccgggcgc agccacggca gagtgccgtt tttacgcact tcagcctgac gctgtaccag
    27661 acggtgtgca taggtgatag gtgctggcat cagcacgtcg gtttcattag ttgcgtagcc
    27721 aaacatcaga ccctggtcac ccgcgccctg ttccagcgga tcggcacggt caacgccctg
    27781 gttgatgtca ggagactgtt tgccgatagc gctcagaacc gcacaggagt tagcgtcaaa
    27841 gcccatgtcg gaatgcacat agccaatttc gcgaacggtg ttacgggtga tctcttcgat
    27901 gtctacccag gcgctggtgg tgatttcgcc gccaactaaa accatgccgg tttttacgta
    27961 ggtttcgcaa gcaacgcgtg ctttcggatc ctgttcgagg atcgcgtcta aaacggcatc
    28021 agagatttgg tcagcaattt tgtcaggatg cccttcagag acggactcgg acgtaaaaag
    28081 gtgttttgcc atatttaata tcacctaaag agaatttggt tagctcaaac tgttgtgtgg
    28141 attttctgtg gtagcggatc ctaccacgac tctgcaagtt aaaaacactg gcagtctgag
    28201 tgttaatcgg tatggatgga ttaacatctg gatggctatt ttaggtcaat tcttcacccg
    28261 atttccactt ttttttgaat cgtgtctcat tctgttaaaa acgtggctgg aaatttttcc
    28321 tgacaatgcc ggcattctgc gtatttatct tttgcaattt tctgccattg tggggtataa
    28381 aacgcggcgc gcggcttaaa taaaaagcac acgacgtttc tttcgtgttg ccacttccag
    28441 ccgggttgaa atcagagttt tggcttgtgg gttcgtctta acaggcggcc gtggaggtga
    28501 tacgaaataa tgaaccgttg tctgctgctt aacctgtctc accgttctgg tgaagattcg
    28561 ttccccgcac tctgcatctc tgctttgcat acctgccgat gttataccca tctcggcgct
    28621 tctcaggatt caagagctgg ttacagttac tgaggactga acaagggcgc tcttgtaaaa
    28681 acaagagttt tctcgtggtt tcgccgaact gtcacactta cgttcggtta tgtgcttaat
    28741 aatgttatga aaaagaaacc ggttgcgcag ttggagcgtc agcattcact gctggaaaat
    28801 ccatgtgctt atgggttgtt atcgcagttc caggctgcga tagtcgttaa ctgttttaca
    28861 cttaataaaa taatttgagg ttcgctatgt ctgacgacat gtctatgggt ttgccttcgt
    28921 cagcgggcga acacggtgta ctacgctcca tgcaggaggt tgcaatgagc tcccaggaag
    28981 ccagcaagat gctgcgtact tacaatattg cctggtgggg caataactac tatgacgtta
    29041 acgagctggg ccacatcagc gtgtgcccgg acccggacgt cccggaagct cgcgtcgatc
    29101 tcgcgcagtt agtgaaaact cgtgaagcac agggtcagcg tctgcctgca ctgttctgtt
    29161 tcccacagat cctgcagcac cgtttgcgtt ccattaacgc cgcgttcaaa cgtgcgcggg
    29221 aatcctacgg ctataacggc gattacttcc ttgtttatcc gatcaaagtt aaccagcacc
    29281 gccgcgtgat tgagtccctg attcattctg gcgaaccgtt ggggctggaa gcaggctcga
    29341 aagcagagtt gatggcggtg ctggcgcatg ctggcatgac ccgtagcgtc atcgtctgca
    29401 acggttataa agaccgcgaa tatatccgcc tggcattaat tggcgagaag atggggcaca
    29461 aggtctatct ggtcattgag aagatgtcag aaatcgccat tgtgctggat gaagcagaac
    29521 gtctgaatgt cgttcctcgt ctgggcgtgc gtgcacgtct ggcttcgcag ggctcgggta
    29581 aatggcagtc ctccggcggg gaaaaatcga agttcggcct ggctgcgact caggtactgc
    29641 aacttgttga aaccctgcgt gaagccgggc gtctcgacag cctgcaactg ctgcacttcc
    29701 acctcggttc gcagatggcg aatattcgcg atatcgcgac aggcgttcgt gaatccgcgc
    29761 gtttctatgt ggaactgcac aagctgggcg tcaatattca gtgcttcgac gtcggcggcg
    29821 gtctgggcgt ggactatgaa ggcacccgtt cgcagtccga ctgttcggtg aactacggcc
    29881 tcaatgaata tgccaacaac atcatctggg cgattggtga tgcgtgtgaa gaaaacggtc
    29941 tgccgcatcc gacggtaatc accgaatcgg gtcgtgcggt gactgcgcat cacaccgtgc
    30001 tggtatctaa tatcatcggc gtggaacgta acgaatacac ggtgccgacc gcgcctgcag
    30061 aagatgcgcc gcgcgcgctg caaagcatgt gggaaacctg gcaggagatg cacgaaccgg
    30121 gaactcgccg ttctctgcgt gaatggttac acgacagtca gatggatctg cacgacattc
    30181 atatcggcta ctcttccggc acctttagcc tgcaagaacg tgcatgggca gaacaactct
    30241 atctgagcat gtgccatgaa gtgcagaagc aactggatcc gcaaaaccgt gctcatcgtc
    30301 cgattatcga cgagctgcag gaacgtatgg cggacaaaat gtacgtcaac ttctcgctgt
    30361 tccagtcgat gccggatgcg tgggggatcg accagttgtt cccggttctg ccgctggaag
    30421 ggttggatca agtgccggaa cgtcgcgctg tgctgctgga tattacctgt gactctgacg
    30481 gtgctatcga ccactatatt gatggtgatg gtattgccac gacaatgcca atgccggagt
    30541 acgatccaga gaatccgccg atgctcggtt tctttatggt cggcgcatat caggagatcc
    30601 tcggcaatat gcacaacctg ttcggtgata ccgaagcggt tgacgtgttc gtgttccctg
    30661 acggtagcgt agaagtagaa ctgtctgacg aaggcgatac cgtggcggac atgctgcaat
    30721 atgtacaact cgatccgaaa acgctgttaa cccagttccg cgatcaagtg aagaaaaccg
    30781 atcttgatgc tgaactgcaa caacagttcc ttgaagagtt cgaggcaggt ttgtacggtt
    30841 atacctatct tgaagatgaa taattaagat tattccgctg gcgggtcgat ttatttacgt
    30901 aaatcggccc gctttctttt tatatattct taaaaataat ctcgctgctt ttcttattaa
    30961 ataatctccc tacaatcgct gcattaatta aatgcagagg gaacggttat gaaaaaatgg
    31021 aaagtacgta gcgcgttggt ggcattaatc gtgctgctgg ccggttgttc cagtaatgcg
    31081 cagtataata gcagtgcatc gggcaatgtc ggcactgcct ggggtgggga tgtgcactca
    31141 acggttcagg gcgtatctgc tgagcgagcc tggcgcgatc cggcagagat gatcgttatt
    31201 agttattcga ctaatgttcc ttccggctac gaccgcgttt atagcattcg tattaacgaa
    31261 ctggaatatg ctattcgcga tggtaatttt aattctctac ctattacgcg ggtttatgat
    31321 tcatccaata atgaaccacg ctatattgtc catgccagag tcggaatgaa ttatcaatta
    31381 tatgtccgta attacagtcg taacaccaac tacgagatcg ttgctacggt tgatggcatg
    31441 gatgtgctga acggcaagca aggttcgctg aataacaacg gctatattgt gaatgccgga
    31501 gattcgttag cgattaaagg attccgcaaa gataaacata ccgaagcggc ttttcagttc
    31561 gccaacgttg ctgattccta cgcagccaat tcggcgcaag gcgatgtgcg taataccggg
    31621 gtaatcggct ttgccgcctt tgaattacaa ggcccggcgc aaaacgcgct gccgccgtgc
    31681 tctggacaag cctttccggc agataacaat ggctatgcgc cgccaccgtg tcggaaatag
    31741 caacctcgct tgatgcctcg cccatttaag gcgataatta gcacaaactt gtgtttcgca
    31801 aatccaaccc ttcctcgtcg ggcctaacga cgcggaaggg tttttttata tcgactttgt
    31861 aataggagtc catccatgag caccttaggt catcaatacg ataactcact ggtttccaac
    31921 gcctttggtt ttttacgcct gccgatgaac ttccagccgt atgacagcga tgccgactgg
    31981 gtgatcaccg gcgtgccgtt cgacatggcc acctctggcc gtgccggtgg tcgtcacggt
    32041 ccggcagcga tccgtcaggt ttcgacgaat ctggcctggg aacacaaccg cttcccgtgg
    32101 aatttcgaca tgcgtgagcg cctgaacgtc gtggactgcg gtgatctggt atatgccttc
    32161 ggcgatgccc gtgagatgag cgaaaaactg caggcacacg ccgagaagtt gctggctgcc
    32221 ggtaagcgta tgctctcttt cggtggtgac cactttgtta cgctgccgct gctgcgtgct
    32281 catgctaagc atttcggcaa aatggcgctg gtacacttcg acgcccacac cgatacctac
    32341 gcgaacggtt gtgaatttga ccacggcacc atgttctata ccgcgccgaa agaaggtctg
    32401 atcgacccga atcattccgt gcagattggt attcgtaccg agtttgataa agacaacggc
    32461 tttaccgtac tggacgcctg ccaggtgaac gatcgtagcg tggatgacgt tatcgcccag
    32521 gtgaaacaga ttgtgggtga tatgccggtt tacctgacct ttgatatcga ctgcctggat
    32581 cctgcttttg caccaggcac cggtacgcca gtgattggcg gcctgacctc cgatcgcgct
    32641 attaaactgg tacgcggcct gaaagatctc aacatcgttg ggatggacgt agtggaagtg
    32701 gctccggcat atgatcagtc ggaaatcacc gctctggctg cggcgacgct ggcgctggag
    32761 atgctgtata ttcaggcggc gaaaaagggc gagtaagcac cagatgcgat gcgcacgggt
    32821 aaaacgtgcc attaatgtcg gatgcggcgt gaacgcctta tccgacctac gttcggcacc
    32881 cgtaggccgg ataagatgcg ccagcatcgc atccggcaat gcgcacaagg taacaaatgt
    32941 gccattcatg tcagatgcgg cgtgaacgcc ttatctgacc tacgttcgac accaccaggc
    33001 tttacttaac cccatccgcg ctcatacgat cgcgaatatg ctgggcgcgt tcggcggatg
    33061 ccggatggtc gtcaaacatt gagctttggc ggccttcttc cagtttcgcc agtttttcaa
    33121 agctggtggc aagacctgcc gggctgatgc cgcgttggcg tagaagatcg taagaataat
    33181 catcggcttc tgcttcctgg cgctgggaga attgcgaatt gactaatttc tcgcccagat
    33241 caccaagttg tgattgagat aaactgccga caatcccgcc cgcagaggcc gcagctactc
    33301 gcacggcatt tgtaccaagc gccacctgca tccctttctt cacatggcct aacgccacgt
    33361 gccccatctc gtgaccgatc accgcttcga cttcgttatc cgtcatcata tccatcagcc
    33421 cgctatagac gcggatacag ccgttagcca ttgcaaaggc gttcacatcc ttcgccatat
    33481 acactttgta attcaccggc tgaccgttga tattgttgcc tagcgcattg gcaatagttg
    33541 tcagacgttt agcgtattcg ctattggctg gcgcaatcgt cgccttgctg tccatctcct
    33601 gacatgcctg atcgctcagg gctttcacct gcgcatcact caaactgtaa gcctgaaaag
    33661 cttccgctcc tgatgaaagc agtccgttgg agtccatatt ctggcaaccg gtcagtaccg
    33721 ttgccacgct cattgctacc aataaggcgc gaattttcat gttttctctt ccgcacaagg
    33781 tcaggattat tctaaaaatg cgccgtttgc aggtgaatcg acgctcagtc tcagtataag
    33841 gaaaagcgca gcggacgggc gagtagattg cgcaacatgc gagcatgatc cagagatttc
    33901 tgaagcggca aaaggatgtt ccatgtacat gacgcgcggc ttgcggtaaa ttgttggcaa
    33961 attttccggc gtagcccaaa acgcgctgtc gtcaagtcgt taagggcgtg cccttcatca
    34021 tccgatctgg agtcaaaatg tcctcacgta aagagcttgc caatgctatt cgtgcgctga
    34081 gcatggacgc agtacagaaa gccaaatccg gtcacccggg tgcccctatg ggtatggctg
    34141 acattgccga agtcctgtgg cgtgatttcc tgaaacacaa cccgcagaat ccgtcctggg
    34201 ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc catgctgatc tacagcctgc
    34261 tgcacctcac cggttacgat ctgccgatgg aagaactgaa aaacttccgt cagctgcact
    34321 ctaaaactcc gggccacccg gaagtgggtt acaccgctgg tgtggaaacc accaccggtc
    34381 cgctggggca gggtattgcc aacgcagtag gtatggcgat tgcagaaaaa acgctggcgg
    34441 cgcagtttaa ccgtccgggc cacgacattg tcgaccacta cacctacgcc ttcatgggcg
    34501 acggctgcat gatggaaggc atctcccacg aagtttgctc tctggcgggt acgctgaagc
    34561 tgggtaaact gattgcgttc tacgatgaca acggtatctc tattgatggt cacgttgaag
    34621 gctggttcac cgacgacacc gcaatgcgtt tcgaagctta cggctggcac gttattcgcg
    34681 acatcgacgg tcatgacgcg gcatccatca aacgcgcagt agaagaagcg cgcgcagtga
    34741 ctgacaaacc gtccctgctg atgtgcaaaa ccatcatcgg tttcggttcc ccgaacaaag
    34801 ccggtactca cgactcccac ggtgcgccgc tgggcgacgc tgaaattgcc ctgacccgcg
    34861 aacagctggg ctggaaatat gcaccgttcg aaatcccgtc tgaaatctat gctcagtggg
    34921 atgcgaaaga agcaggccag gcgaaagaat ccgcatggaa cgagaaattc gctgcttacg
    34981 cgaaagctta tccgcaggaa gccgctgaat ttacccgccg tatgaaaggc gaaatgccgt
    35041 ctgacttcga cgcgaaagca aaagagttca ttgctaaact gcaggctaac ccggcgaaaa
    35101 tcgccagccg taaagcgtct cagaatgcta tcgaagcttt cggtccgctg ttgccggaat
    35161 tcctcggcgg ttctgctgac ctggcaccgt ctaacctgac cctgtggtct ggttctaaag
    35221 caatcaacga agatgctgcg ggtaactaca tccactacgg tgttcgcgag ttcggtatga
    35281 ccgcgattgc taacggtatc tccctgcacg gtggtttcct gccgtacacc tccaccttcc
    35341 tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc tgcgctgatg aaacagcgtc
    35401 aggtgatggt ttacacccac gactccatcg gtctgggcga agacggcccg actcaccagc
    35461 cggttgagca ggtcgcttct ctgcgcgtaa ccccgaacat gtctacatgg cgtccgtgtg
    35521 accaggttga atccgcggtc gcgtggaaat acggtgttga gcgtcaggac ggcccgaccg
    35581 cactgatcct ctcccgtcag aacctggcgc agcaggaacg aactgaagag caactggcaa
    35641 acatcgcgcg cggtggttat gtgctgaaag actgcgccgg tcagccggaa ctgattttca
    35701 tcgctaccgg ttcagaagtt gaactggctg ttgctgccta cgaaaaactg actgccgaag
    35761 gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc atttgacaag caggatgctg
    35821 cttaccgtga atccgtactg ccgaaagcgg ttactgcacg cgttgctgta gaagcgggta
    35881 ttgctgacta ctggtacaag tatgttggcc tgaacggtgc tatcgtcggt atgaccacct
    35941 tcggtgaatc tgctccggca gagctgctgt ttgaagagtt cggcttcact gttgataacg
    36001 ttgttgcgaa agcaaaagaa ctgctgtaat tagcatttcg ggtaaaaagg tcgcttcggc
    36061 gacctttttt attaccttga tatgtccgtt tgcggacaag caatagataa agcgtgttgt
    36121 agatcacaaa tatttatatg caataaatat caattatgta a
//
LOCUS       DAPHJE010000041        33289 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPHJE010000041 DAPHJE010000000
VERSION     DAPHJE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.041"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            78..1460
                     /locus_tag="RH222_002018"
     CDS             78..1460
                     /locus_tag="RH222_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV3797039.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1470..3788
                     /gene="yicI"
                     /locus_tag="RH222_002019"
     CDS             1470..3788
                     /gene="yicI"
                     /locus_tag="RH222_002019"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV3797040.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(3841..5550)
                     /locus_tag="RH222_002020"
     CDS             complement(3841..5550)
                     /locus_tag="RH222_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV3797041.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(5671..7062)
                     /gene="xanP"
                     /locus_tag="RH222_002021"
     CDS             complement(5671..7062)
                     /gene="xanP"
                     /locus_tag="RH222_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV3797042.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVLSRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7342..8547
                     /gene="gltS"
                     /locus_tag="RH222_002022"
     CDS             7342..8547
                     /gene="gltS"
                     /locus_tag="RH222_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV3797043.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            8550..9416
                     /locus_tag="RH222_002023"
     CDS             8550..9416
                     /locus_tag="RH222_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797044.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYAAAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(9401..11482)
                     /gene="recG"
                     /locus_tag="RH222_002024"
     CDS             complement(9401..11482)
                     /gene="recG"
                     /locus_tag="RH222_002024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV3797045.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELQLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11488..12177)
                     /gene="trmH"
                     /locus_tag="RH222_002025"
     CDS             complement(11488..12177)
                     /gene="trmH"
                     /locus_tag="RH222_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV3797046.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12184..14292)
                     /gene="spoT"
                     /locus_tag="RH222_002026"
     CDS             complement(12184..14292)
                     /gene="spoT"
                     /locus_tag="RH222_002026"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV3797047.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14311..14586)
                     /gene="rpoZ"
                     /locus_tag="RH222_002027"
     CDS             complement(14311..14586)
                     /gene="rpoZ"
                     /locus_tag="RH222_002027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV3797048.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14641..15264)
                     /gene="gmk"
                     /locus_tag="RH222_002028"
     CDS             complement(14641..15264)
                     /gene="gmk"
                     /locus_tag="RH222_002028"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV3797049.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15522..17204
                     /gene="ligB"
                     /locus_tag="RH222_002029"
     CDS             15522..17204
                     /gene="ligB"
                     /locus_tag="RH222_002029"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV3797050.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17201..17818)
                     /locus_tag="RH222_002030"
     CDS             complement(17201..17818)
                     /locus_tag="RH222_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV3797051.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18109..18711)
                     /gene="dinD"
                     /locus_tag="RH222_002031"
     CDS             complement(18109..18711)
                     /gene="dinD"
                     /locus_tag="RH222_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDV3797052.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(18932..19795)
                     /locus_tag="RH222_002032"
     CDS             complement(18932..19795)
                     /locus_tag="RH222_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV3797053.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            19922..20638
                     /gene="rph"
                     /locus_tag="RH222_002033"
     CDS             19922..20638
                     /gene="rph"
                     /locus_tag="RH222_002033"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV3797054.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            20704..21345
                     /gene="pyrE"
                     /locus_tag="RH222_002034"
     CDS             20704..21345
                     /gene="pyrE"
                     /locus_tag="RH222_002034"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV3797055.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            21643..22602
                     /locus_tag="RH222_002035"
     CDS             21643..22602
                     /locus_tag="RH222_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDV3797056.1"
                     /translation="MDATRTTLLALDLFGSPGWSVDKEIQRLHALSNHAGRHYRRIIL
                     SKRHGGQRLVLAPDYLLKTVQRNILKNVLSQFPLSPFATAYRPGCPIVSNAQPHCQQP
                     QILKLDIENFFDSISWLQVWRVFRQAQLPRNVVTMLTWICCYNDALPQGAPTSPAISN
                     LVMRRFDERIGEWCQARGITYTRYCDDMTFSGHFNARQVKNKVCGLLAELGLSLNKRK
                     GCLIAACKRQQVTGIVVNHKPQLAREARRALRQEVHLCQKYGVISHLSHRGELDPSGD
                     LHAQATAYLYALQGRINWLLQINPEDEAFQQARESVKRMLVAW"
     gene            complement(22648..23244)
                     /gene="slmA"
                     /locus_tag="RH222_002036"
     CDS             complement(22648..23244)
                     /gene="slmA"
                     /locus_tag="RH222_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV3797057.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23351..23809)
                     /gene="dut"
                     /locus_tag="RH222_002037"
     CDS             complement(23351..23809)
                     /gene="dut"
                     /locus_tag="RH222_002037"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV3797058.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23787..25007)
                     /gene="coaBC"
                     /locus_tag="RH222_002038"
     CDS             complement(23787..25007)
                     /gene="coaBC"
                     /locus_tag="RH222_002038"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV3797059.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPARVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDEFTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25179..25847
                     /gene="radC"
                     /locus_tag="RH222_002039"
     CDS             25179..25847
                     /gene="radC"
                     /locus_tag="RH222_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV3797060.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26064..26300
                     /gene="rpmB"
                     /locus_tag="RH222_002040"
     CDS             26064..26300
                     /gene="rpmB"
                     /locus_tag="RH222_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV3797061.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26321..26488
                     /gene="rpmG"
                     /locus_tag="RH222_002041"
     CDS             26321..26488
                     /gene="rpmG"
                     /locus_tag="RH222_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV3797062.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26586..27395
                     /gene="mutM"
                     /locus_tag="RH222_002042"
     CDS             26586..27395
                     /gene="mutM"
                     /locus_tag="RH222_002042"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV3797063.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27434..27913)
                     /gene="coaD"
                     /locus_tag="RH222_002043"
     CDS             complement(27434..27913)
                     /gene="coaD"
                     /locus_tag="RH222_002043"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV3797064.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27921..29198)
                     /gene="waaA"
                     /locus_tag="RH222_002044"
     CDS             complement(27921..29198)
                     /gene="waaA"
                     /locus_tag="RH222_002044"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV3797065.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29611..30669
                     /gene="rfaQ"
                     /locus_tag="RH222_002045"
     CDS             29611..30669
                     /gene="rfaQ"
                     /locus_tag="RH222_002045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV3797066.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30666..31790
                     /locus_tag="RH222_002046"
     CDS             30666..31790
                     /locus_tag="RH222_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV3797067.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31783..32580
                     /gene="rfaP"
                     /locus_tag="RH222_002047"
     CDS             31783..32580
                     /gene="rfaP"
                     /locus_tag="RH222_002047"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV3797068.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            32596..>33289
                     /locus_tag="RH222_002048"
     CDS             32596..>33289
                     /locus_tag="RH222_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HDV3797069.1"
                     /translation="MSAHYFNPQEMITKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKVLYLDADIACQGNLKPLITMDLANNIAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGK"
BASE COUNT         7814 a         8240 c         8950 g         8285 t
ORIGIN      
        1 gaatattcgc tctcacaggg atggtttaca aaatgcgtta tcggtctcag cacccctata
       61 gcatcaagga aaagcagatg aagagtgaag tgttgtccgt taaagagaaa attggttatg
      121 gcatgggaga cgccgccagc cacattattt tcgataacgt aatgttatat atgatgttct
      181 tttataccga tatttttggc attcctgccg gttttgtcgg aaccatgttt ttggtcgctc
      241 gtgcactgga tgcgatttcc gatccttgca tggggttgtt ggccgatcga acgcgctctc
      301 gctggggtaa atttcgtccg tgggtactgt ttggcgcact gccattcggg atcgtctgtg
      361 tactggccta tagcacgcca gatctcagta tgaacggcaa aatgatctat gcagcaatta
      421 cttacaccct acttacctta ctttataccg tcgtcaatat cccttactgc gcattgggtg
      481 gtgtaatcac caatgacccg actcagcgta tctcgctgca atcctggcgt tttgtgctgg
      541 cgaccgcggg aggcatgctt tctactgttc tgatgatgcc actggttaat ttaattggcg
      601 gtgataataa accactcggt ttccagggcg gtatcgcggt cctttccgtg gtggcattca
      661 tgatgctggc attttgtttc ttcaccacta aagaacgcgt tgaagcacca cctacaacaa
      721 cgtctatgcg ggaagattta cgtgatatct ggcaaaacga ccagtggcgg attgtcggtt
      781 tactaaccat tttcaatatc ctggcggtgt gcgtacgcgg tggggcgatg atgtattacg
      841 tcacatggat tttgggcacg ccggaagtgt ttgtcgcttt tctcaccact tattgcgtgg
      901 gtaacctgat tggttccgca ctggcaaaac ctctgaccga ctggaaatgt aaagtcacta
      961 tcttctggtg gacgaacgcc ctgctggcag tgattagcct cgcgatgttc tttgttccca
     1021 tgcaggccag catcactatg tttgtcttca tcttcgtgat tggtgtgttg catcaactgg
     1081 tgacacctat ccagtgggta atgatgtccg ataccgtcga ctacggcgag tggtgcaatg
     1141 gtaaacgcct gaccgggatc agttttgctg gcacgctgtt tgtgctcaaa ctggggttgg
     1201 ccttcggcgg cgctcttatc ggctggatgc tggcttatgg cggatatgat gcggcagaaa
     1261 aagcgcagaa cagcgccacg attagcatca ttattgcgct attcacgatt gttccggcga
     1321 tctgttattt gctgagcgcg attatcgcta aacgctacta ctcactcacg acgcacaatc
     1381 tgaaaaccgt tatggaacag ctggctcagg gtaaacgccg ttgccagcaa caattcacct
     1441 ctcaagaagt gcagaactaa ggaacggcaa tgaaaattag cgatggaaac tggttgattc
     1501 aacctggcct caatttgatt cacccgcttc aggtgttcga ggttgaacag caggataatg
     1561 aaatggtggt ctatgctgcc ccccgtgatg tgcgtgaacg tacctggcag cttgatacgc
     1621 ctttatttac gttgcgcttt ttctccccac aggaaggtat tgtcggtgtg cggattgagc
     1681 attttcaggg ggcgctgaat aacggtcctc attatccgct caatattttg caggacgtga
     1741 aggtcacaat cgaaaacaca gaacgttatg ctgagtttaa aagtggcaac ttaagcgcgc
     1801 gtgtcagcaa aggtgagttc tggtcactgg attttctgcg caacggcgaa cgtattaccg
     1861 gtagtcaggt gaaaaataat ggctacgtgc aggacacgaa taatcaacgc aattatatgt
     1921 ttgagcggct tgatcttggc gttggcgaaa cagtttacgg tctgggagag cgctttactg
     1981 ccctggtgcg caatggccag acggtagaga cctggaaccg ggacggcggc acaagtactg
     2041 aacaggcgta taaaaatatc ccgttctaca tgactaaccg tggttatggg gtactggtca
     2101 atcatcccca gtgtgtctct tttgaagtgg gatcggagaa agtctccaaa gtgcagttca
     2161 gcgttgagag tgaatatctc gaatactttg ttatcgacgg cccgacgccg aaagcggtac
     2221 ttgatcgtta tacccgcttt actggtcgtc cggcgctgcc gcccgcgtgg tccttcggcc
     2281 tgtggctaac cacttcattt accaccaact acgacgaagc gacggtaaac agctttatcg
     2341 atggtatggc ggaacgcaat ctgccgctgc atgttttcca ctttgactgt ttctggatga
     2401 aagccttcca gtggtgcgat tttgagtggg acccgctgac tttccctgac ccggaaggga
     2461 tgatccgccg cctgaaagcg aaagggctga aaatctgcgt ctggattaac ccctatatcg
     2521 gtcaaaaatc ccccgtcttt aaagagttac aagagaaagg ctatttactc aaacgccccg
     2581 acggttcgct atggcagtgg gataaatggc agccaggtct ggcgatttat gactttacca
     2641 atccggatgc ctgcaaatgg tacgccgaca aactgaaagg tctggtcgcg atgggcgttg
     2701 attgctttaa gaccgacttt ggcgaacgta tcccaactga tgttcagtgg tttgacggtt
     2761 ccgatccgca gaaaatgcat aaccattatg cgtacatcta caacgaactg gtgtggaacg
     2821 tgctcaagga caccgttggt gaggaagaag ctgtcttgtt tgcccgctcg gcctccgtcg
     2881 gtgcgcagaa attcccggta cactggggtg gcgattgtta cgctaactac gaatcaatgg
     2941 cggaaagcct gcgcggtggt ttgtctattg gcctttcagg ttttggcttc tggagccacg
     3001 atatcggcgg ctttgaaaat accgctccgg cgcacgttta caaacgctgg tgcgcgtttg
     3061 gtttgctctc cagccatagc cgtttacacg gtagcaaatc ttatcgtgtg ccgtgggcct
     3121 acgatgatga gtcctgtgat gtggtgcgct tcttcacgca actgaaatgc cgcatgatgc
     3181 cgtatctgta tcgtgaagct gcgcgtgcga acgcgcgggg tacgccgatg atgcgggcca
     3241 tgatgatgga gttcccggac gatccggctt gtgattacct tgaccgtcaa tacatgttag
     3301 gcgacaacgt gatggttgcg ccggtgttca ctgaagcggg cgatgtgcag ttctacctgc
     3361 cggaaggtcg ctggacacac ctgtggcaca acgatgaact cgacggtagt cgctggcata
     3421 aacagcagca cggcttcctg agtctgcccg tttatgtgcg tgataacact ctactggcgc
     3481 tgggcaacaa cgatcaacgt cccgattacg tgtggcacga aggcacggca ttccacctct
     3541 ttaatctgca agacgggcat gaagccgtct gtgaagtgcc cgctactgac ggatcggtga
     3601 tctttacttt aaaagcagca cgtactggca acacgattac tgtgactggt gcgggcgagg
     3661 cgaagaactg gacgctgtgc ctgcgcaatg ttgtgaaagt aaatggtctg caagacggtt
     3721 cgcaggctga aagtgagcag gggctggtgg tgaagcctca agggaatgcg ctgacaatta
     3781 cgttgtaatc tattgatgga atgccgggta cgtctgagtc gtacccggcg agattgatgt
     3841 ttaccgcacc ggctccgtcg atgtaacttc accgctggaa acgatgccag cattcatggt
     3901 ttgagtcact tgctgctttg cggcgttcac ggcatgcaat tgcccgctaa ccgtaggttt
     3961 caacgggaca ttggcctgaa tatcgccact ggcggtaagc tgaatattgc cgtcgccagt
     4021 caacggtaat ttaggccatc cccactgttg caaaatattc accggcacac cgcgtccatt
     4081 caggctgata tgtgtctgac gttgtggcgt ttgtgaaaca ctggcggtgg cttccagaat
     4141 gcctttttca gtaaatgcac tcagttcgct gatattcacc gtgctgctgt tggcggtcag
     4201 cgccagcgac gggcgacgaa catcaacacg attgaatgtc gcggcggcgg cattcagatt
     4261 cgccgagcca ctccagacgc cccatttatg atcggtaacc agcgtcaggt tggcaccgta
     4321 accatcgagc gtggtgagct gccacgggaa gtcagggtcg atatcaatga tcagattgcg
     4381 gctggcgcta aatctcttca gttgcaggct gtttaaccaa ccgggtgtcg tttccatcca
     4441 caactgttgc cagtttttcg gcaaggtata ttccagcccg gcaattgccg catcatcaag
     4501 gatcaacgtt ttcccgtcac gcagccagtt ccctgacgtt ctgaccatac ccccttccca
     4561 gcggctggtg aactggcgca gcgctacgcc ctgcggggaa aattccgcgt ttataatcgg
     4621 gtcaaataaa tgcagcgaac cataaatgaa ctcgctagcg ttcatcgaca gtttgccttc
     4681 ttgtgtctgc cagtcatctt tactgaaggt catgttgcgc aagctgagat cgaggtcggt
     4741 caccgcccag tccggacctt gcaaacgagc atcgatcact tccaggcgac caatctgcaa
     4801 cgacgggaca gagcgtaatg gcgcaaagaa gtcggttagc gatttttcgc tttgtagacg
     4861 tatatccgcc atgcgcaggt tttccacttg ccagctgccg tcggcgttac gctgcgcgtt
     4921 tccggttaat gtcccgcggg cgatgtcggc acccaggtta gtcagcgtaa cgcgatcatt
     4981 atcaatactg ccttcaatca gtacattggt ggcaggaaca tcattgagcg aaagcgatcc
     5041 ggcactaaac tgaatctgcg ccttcgtacc cagcacttta ccggcttctg gtgaccacgg
     5101 aaccacgccg ccatttaccc gctgcgcgct cagtttccat tcgctattcg ggctattaaa
     5161 cgccatatca cgcagttgca gacgatcggc tttgaacggt agcggcgcgg tctggtcggt
     5221 gagattcagc gtcccgtttt ccagcaggat ggtatcgaca tggcgtggtt cggtcagttg
     5281 ccgactgctt agcgcaatgt cgacactttt tgccaccagg gtcgcgggct gaccatcacg
     5341 accaaacgtg acgttctcca gcacgatatg agatggcgcg gaaaaacggt gatccatcgc
     5401 cccgaaggcc agatgatagt cgctattctc ggaaacccat gcgctgatat gttctgctcc
     5461 ccagcgggtt tgcagaagaa aatagaggcc agcgatcacc actaacagag cgatgagaat
     5521 gtagagaagc agcttcccaa taaatttcat ggtcttccat cccgtgaaat gcacataagg
     5581 gagttatgca cgatttacgc gcaatcctca aggcgggaat ggtgaaagag gtgagactgc
     5641 ggcggtaatt atcttaccgc cgccagtgaa ttactgtttt tctggtggga aaatcagatt
     5701 cagcacgatg gcagtaatac cgcccgcggc gatcccggag gagagcaggt ttttcagcca
     5761 ttcaggggca aactgcaaaa tcagcggctg ctgagacacg cccagaccaa ccgccagcga
     5821 cagcgcgata atcagaatcg cccgacggtt cagcggctca cgagaaacga tacgcacacc
     5881 ggaggcggcg atggtgccaa acattacaag cgttgcgccg cccagaaccg gttctggaat
     5941 gtgttgtaca aaaccgctca ctgccgggaa cagacccagc acgatcaaca tcagcgcgac
     6001 gacaaaaccg acatagcggc tggcaacacc agtcaactgg atcactccgt tgttttgccc
     6061 gaagcaggag ttcgggaagg tgttaaacac cgccgaaaca aacgagttca ggccgtttgc
     6121 cagcacgccg cctttcaggc gtttcatgta cagcggaccg gacactggct gttcggaaac
     6181 gtcagaggtc gccgtgatat cgccaatggt ttccagcgaa gtgatcataa agaccagcat
     6241 cagcggcagc agcagactcc attcaatgcc aagaccgtaa tagagcggcg ttggcaccat
     6301 aatcagttct tgcgtcatcg gttcgttgct ttctggcaac atgcccataa accacgccag
     6361 cgcatatccg gccgccatcg caatcaccag tgaggccacg cgtaagtaag ggttacgttg
     6421 acggttaagc aggataatta aggctaagac cacgcctgcc agcagcagat ttttcggtgc
     6481 gccgaaggtg ttatcgctca tggctgcgta accgccgcca atggacgtta acccaacctg
     6541 aattagcgac aggccgataa tcatcaccac aacgccagaa accagcggcg taataatgcg
     6601 gcgcgccaga tgcagaacgc gggagagcac catctcggtg caacttgcca gcatcaacgt
     6661 gccgaacaaa gccgccatca tggtaggaac atcagcacca ccggttttca gcgcggtacc
     6721 gcccataatc agcggggcaa caaagttgaa gctggtgccc tgaatagaca acagcccgga
     6781 gccaaccgga ccccaggcct taatttgaat aatcgatgcc acaccggagg caaacagcga
     6841 catactaata atgtgttgcg tgtcttgtgc cggtaaaccc agcgcctggc agattaatag
     6901 cgctggcgtg atcaccgcaa cgaacatcgc cagcagatgc tgacaggcgg caaacagggt
     6961 ttgaggaagc ggcggacgat cttcaagacg gtaaatcagt tcgctgtttt gagtctgcgc
     7021 aaccggttgc gcattttctg actcgagggt ggaaacagac atcgaaaaca atcccgtggt
     7081 agcaaagcgg gcattttagc tgaccgaaag agaaaagcaa acgtttgcca catcctcatc
     7141 taaatattta aatgagataa atgcactttt ttatataact tttggtattt ttctgcttaa
     7201 aatccatgcc atttgttcat taacagctga gaatttgacc caggaaagca taactcgata
     7261 cccgtcatag ttcatgaagt tgccactgcg cgatgaagta tgacgagtat gaaagagtga
     7321 tgcggataca aaggagtaac tatgtttcat ctcgatactt tagcaacgct tgttgccgca
     7381 acgctgacgt tgctgctcgg gcgtaagttg gtccattccg tctccttttt gaagaaatac
     7441 accataccgg aacctgttgc gggtggtttg ttggtggcgc tggcgctact agtactgaaa
     7501 aaaagcatgg gctgggaagt caactttgat atgtccctgc gcgatccgtt aatgctggct
     7561 ttcttcgcca ccattggcct gaacgccaac attgccagtt tgcgtgccgg tgggcgtgtg
     7621 gttggcatct tcttgattgt ggttgttggt ctgttggtga tgcaaaatgc cattggcatt
     7681 ggtatggcta gcctgttagg gcttgatccg ctgatggggc tgttggccgg ttctattacg
     7741 ctttccggcg gtcacggtac gggcgctgcg tggagtaaat tgttcattga acgttatggc
     7801 ttcaccaatg cgacggaagt ggcgatggcc tgtgcaacgt tcggtctggt gctgggcggc
     7861 ttgattggcg gtccggtggc gcgctatctg gtgaaacact ccaccacgcc gaacggtatt
     7921 ccggatgacc aggaagtccc gacggcgttt gaaaagccgg atgtgggccg catgatcacc
     7981 tcgctggtgc tgattgaaac tatcgcgctg attgctatct gcctgacggt ggggaaaatt
     8041 gttgcgcaac ttttggctgg cactgctttt gaactgccga ccttcgtctg tgtcctgttt
     8101 gttggcgtga ttctgagcaa cggtctgtca atgatgggct tttaccgcgt ctttgagcgt
     8161 gcggtatccg tgctgggtaa cgtaagcctg tcgttgttcc tggcgatggc gctgatgggg
     8221 ctgaaactgt gggagctggc ttcgctggcg ctgccgatgc tggcgattct ggtggtacag
     8281 accatcttca tggcgttgta tgccatcttc gttacctggc gcatgatggg caaaaactac
     8341 gatgcggcag tgctggctgc gggtcactgt ggttttggcc tcggtgcaac gccaacggca
     8401 atcgccaaca tgcaggcgat cactgaacgc tttggcccgt cgcacatggc gtttttggtg
     8461 gtgccgatgg tcggtgcgtt ctttatcgat atcgtcaatg cgctggtgat taagctgtat
     8521 ttaatgttgc cgatttttgc cggttaaaaa tgaaaaagcg tcggaaggaa ccggaaacgc
     8581 tacgtgaaca ctgtcgccat atttttggtg atgagccgcc agtgctctgt gtatgggaaa
     8641 cggaatttga ttacgcagct gccgaactca aggcgctggc ggctaaagag tggcagcaga
     8701 tcagcgaatg ggatttgagt gcttactatg tactcaatct cgtttataac gaaccgatgc
     8761 aaatagagtt gtttcgctat ctgtttccgc tctgcctggc gcaatggcat gaaaccgttt
     8821 tagctggcgg ttatggtgat cacttcgaag aatccttaat gaaggcattg tgtcggccct
     8881 atctctggca ggagatgatg aatgcttccc aacgccagca ggtacggcaa tttttactgg
     8941 acaccgcgtt gcagcgtatg gataacgagc gtggtttcaa taacgttctt tgctggctgg
     9001 cggtgttcaa tactctgggt ggggccgcgc cgttaattcg ttctctctgg tcgcgctggt
     9061 gggcgctgga tacgccagga aaagcggtgt gtgctattca gtatgccgcg catctaattt
     9121 atcccataga agccaatccg ttatggtcgc aagaatggat aggctggggg catccattag
     9181 gccataaaga tggctggtcc tctgataatc gcgcttttct tcgccaaatg ttaacacctg
     9241 aaatgattgt ggctggcgta caggcagcgg ctgaaattct gcgtggtgaa ccggaaggcg
     9301 caatggcggc gcgaattgca caggatgcgt atgaggcaat ggatatcctc actatccaga
     9361 ttgaagattt actgcgtgac ttgtcttgtg atgagtctgg tcatgcgttg gagtaacgtt
     9421 ccgtctccgg catccagcgt tctatcaggg cttttgcttg ttgtgggtaa cgttcgtgaa
     9481 tatgtcgcgc caggcgctga acttccggga tcatcgcctg atcgcgcagt aaatccgcca
     9541 ctttaaattc agcattaccc gtctgacgcg tgcctaacaa ttcgccaggg ccgcgaatct
     9601 ccagatcttt ttgcgcaatc acaaaaccgt cgttactgtc gcgcagcact tgcaggcgaa
     9661 tttgcgccgt tttggagagc ggcgttttgt agagcagcac gcagtgagaa gccaccgcgc
     9721 cacgacctac gcgcccgcgc agctggtgta actgcgctag acccagacgc tcagggtttt
     9781 cgataatcat cagactggcg ttaggcacat caacgccgac ttcaataacg gtagtggcaa
     9841 ccagcagttg tagctcacct tgtttaaacg acgccatcac cgcctgtttc tcggctggtt
     9901 tcatccgccc gtgtaccagg ccaacattca attctggtag cgccagtttc aactcttccc
     9961 aggtagcttc cgccgcctgc gcttccagta actctgactc ttcaatcaac gtacaaaccc
    10021 agtacgcctg acgaccttca gtcatgcagg cgtggcgcac gcggtcaatg atgtcggtac
    10081 ggcgggtatc aggaatagcg accgtagtca ctggcgtgcg gcctggcggc agctcatcta
    10141 tcaccgaggt atcgagatcg gcatacgccg tcattgccag cgtgcggggg atcggcgtgg
    10201 cggtcatgat caactgatgc ggatggaagc cctgctgttg gcctttctcc cacaatgcca
    10261 gacgctgatg cacgccaaaa cgatgctgtt cgtcgataat caccagcgcc agaccgttaa
    10321 actgcacctg ttcctggaag atggcgtgtg taccgacaat catctgcacc tgaccgctgg
    10381 cgatggcttc ctgctgtgcc agtcgcgctt tacctttctg cttaccggcg agccagccca
    10441 cttcgatacc gagcggtgca aaccagttgc ggaagttatt ggcgtgctgc tcggcgagta
    10501 attcggttgg tgccatcagg gccacctgtt tgccgtgggc aatcgcacgc agcgcggcga
    10561 gggcggcgac cagtgtttta ccggaaccta catcgccctg caccaggcgc atcattggaa
    10621 catccagcgc catatcatgc tcgatctccg ccactacgcg tgcctgtgcg cccgttggct
    10681 tgaacggtaa ggcggcgagg agtttatttt tcagcgcgtc attggcgctc agcggctggg
    10741 catgaaaacg ctgtgctccg gcacgtaagg ctaacatgct gaggttgtgc gccagcagtt
    10801 cttccagaat cagacgacgt tgcgccggat gctgcccggt ttccagatcg ctaagctgta
    10861 gcgtcggcgg cgggcggtgc aaagtgcgca acgcttccgg tagcgtcatc attccttgtg
    10921 acagttccgg cggcaggagt tcttcaatgg cgcaggtgtc gagcagatcc agcgcctggt
    10981 cggttaattt acgcagcgtg gcctgcttta cgccttccgt tgttggataa accggcgtga
    11041 gcgtttcctg taattctggc gtgctgagat cgccctgcac gcggtattcc gggtggatca
    11101 tctccgcacc atatttaccg cgctttgctt cgccataagc cagtacacgg cggcccgtcg
    11161 ccaggctatt tttcattgcc gcgctgaaat tgaaaaagcg catggtgagg atgccggaac
    11221 cgtcgctgat ctggcaggtc atcatccgcc gaccgccgaa ggagatattg cagttcagca
    11281 cttcgccttc caccgtggca taaacgcccg gcagtagttc tccgatgggg tagagatggg
    11341 tgcgatcttc gtagcgcagg ggaaggtgta agagtagatc ctgcacggta tgcaggttga
    11401 ttttcgccag tttgttacta agtgctgcgc caacgcccgt tagggaactg agtgggacag
    11461 catctaacag gcgacctttc atggcactta ccctgcagcc tgcatagtag cccaccagtc
    11521 ggcatcagct tcgatctcgc cttgctgatt gacgtgggga taaggcaggc ctttgcgttt
    11581 tgcgactttc gccagcaccg gatagccgcc ttcaaacaac aggcgttgtt gctctgcttc
    11641 cggcaacatg ctgttttcac gcaggtacat gcctgcattt tgccgctgac gctgggcttc
    11701 gtaaagaatg agggctgagg caacggaaac attcagcgac tgcaccatgc cgatcatcgg
    11761 aatgatgatg tcctgatccg ccagggccaa tgcttcctgc gtgatgcccg ttttctcctg
    11821 tcccatcaaa atgcaggtcg ggcgagtgta atcaatttcg cggaaatcga cagcgttatc
    11881 agaaagatgg gttgccagaa tctgcatgcc ctggcctttg agatgagcga cggcatcgcc
    11941 aatggtgcgg tgtgttttca cctgtaccca gctgttacta cccgccgctg ccgaagccat
    12001 ggtgcgcatg cggctaccag gccagacggc gtgaacttca tgtacgccaa cggcatctgc
    12061 ggtacgaata atcgcagaaa cgttatgagg tttgtggacc tgctccatgc agacggtcag
    12121 atcaggctgc cgcctggcga gcatttcgca gatgcgtgca taacgtgttg ggttcataaa
    12181 acattaattt cggtttcggg tgactttaat cacgtctggc atcacgcgga ttttgcgcat
    12241 gatattcgcc agatgcacac ggtcacgggc ggtcagacga ataaaggcgc tgtagacgcg
    12301 accatctttc tcttccgtat tcaaactttg aatattcgaa gtcgtggtgt taattgccgc
    12361 cgtcaggttt gccagcgcgc cctgatgatt gaacatctcc accttgattt cggtgatgaa
    12421 ctcctgcgcc gtctctttat cccattccac agccataaac ttctctggct ctttctggta
    12481 gccacggata ttacggcagg attcatggtg gatcaccaga cctttaccgg ggctgacgtg
    12541 ggcgataatc gggtcgccag gaatagggcg gcagcatttc gcaaaggtga tcagcacgcc
    12601 atcggcacct ttaatgggca gatgtccgtg gctttgggtt gccggtggaa tggaggcgtc
    12661 cccatgttgc agatttttcg cgaccaccac gctcattgcg ttaccaagtc cgatttctgc
    12721 cagcagatcg tcaagcgttg ccagcttcat gcgatccagc tcgcgctgaa tattttcctg
    12781 cgggatttca ttcagcttac ggctaccacc caaagcatgg ttgagcagac gacggcccag
    12841 gcttacagaa tcatcacgct tgaggttttt cagcaactga cgaattttgg cgcgcgcttt
    12901 tgagctaacg acaaagttca gccacgcggc attcgggcga gcgcccggtg cggtaatgat
    12961 ttcaacggtt tgaccgctgg taagcggctg cgacagcggg taaggctggc ggtcaacgcg
    13021 tgcgcccacg caggcatgac cgatatcggt atgcactgca taagcgaagt cgacgggcgt
    13081 tgcaccggca ggcagctcga caattcgccc ttccggtgtg aaaacgtaaa tctcatccgg
    13141 gaagagatcg gatttaacgc tctcgataaa ttcaaacgaa ctaccggcgc tctgttgcag
    13201 ctccagcagg ctttgcatcc agcgctgggc gcggatttgc gcggtggtgc tggtttcgcc
    13261 gtgctcttta taagcccagt gcgcggcaac acccatctcc gccatctgat ccatatcttc
    13321 ggtacggatc tggacctcaa ccggcacacc gtgcgggccg atcatcgagg tgtgcaaaga
    13381 ctgatagccg ttcgcttttg gaatggcgat gtagtctttc acgcgacccg gacgcggctt
    13441 gtacaggcta tgcatttgcc cgagcacgcg ataacaggtg tcagaatcat tgacgatcac
    13501 gcggaaagcg tagatgtcca tgattgagtg aaaacgctgc tctttgagca ccattttgca
    13561 gtaaatcgaa taaagatgct tttcgcgacc actgacgcgg cacggtattc ccgcttcctg
    13621 caaacgccct tcgatttcag agaggatttt ttggatcatc tctttacggt tgccgcgcgc
    13681 ggctttcacc acttctttaa ttacgcggta acgattggga tacagcgcct caaaacccag
    13741 ctcttcgagt tcggttttaa tgtggtggat acctaaacgg tgcgccagcg ggctataaat
    13801 ttcgagagtt tcacgggcga tgcggcgacg tttgtccggg cgaagtgagc ccagcgtgcg
    13861 catgttgtgg gtacggtcgg caagtttgat gaggatgacg cggatatcct gcaccatcgc
    13921 cataatcatc ttgcgaaagt tttcggcctg cgcctctttc ttatcgcgga acttgagttt
    13981 atcaagtttc gacaccccct ctaccagctc ggcgacgctt ttaccaaaaa gctgttccat
    14041 atcctggtag gtggcgggag tatcttcaat cacgtcatgc agcagcgccg ccatcagcgt
    14101 ttcatagtcg agtttcatct cggccagaat gcaggcaacc gctaccgggt gcgtgatata
    14161 gggttcaccg cttgaacgtg tttgcccctc gtgagcatca cgtgcaacga gatacgcctg
    14221 ccgcagacgc ttgatttggt cttccggcag gtaggtttga atcagttgat tcaggctttc
    14281 aaacagatac aagggcgacc cgctttgtga ttaacgacga ccttcagcaa tagcggtaac
    14341 ggcttgtaat tcagcggctt cctgctcttg ctgttcctgg cgttcgcgaa cgtcgaggat
    14401 ctggttgttg atcagacctt cttcgatttc gcgcagcgcg attacagtgg ttttatcgtt
    14461 ttcttccggt accagcggat cctttccgcc tacctgcatc tgacgagcgc gacgcgcggc
    14521 gaccagtacc aggtcaaaac ggttaccaat tttctctaca gcgtcctgaa cagttacgcg
    14581 tgccatactt aaaaagctcc acaggtgaag aaatgactgg gcatgatact gaaatcaggt
    14641 tcagtctgcc aacaatttgc tgattaaagc gtcatgacgc tgcttttggc ggctcatgcg
    14701 cagacgttcg gcgcgaataa tggtcttcaa atcggtcaac gcggtatcga agtcatcatt
    14761 cacaatcaga taatcatatt cggcgtaatg gctcatttct gcaacagctt gcgccatacg
    14821 ctttgcaatg acctcttcgc tgtcctgacc gcgaccgcgt agacggcggt ccagttcaat
    14881 tttggacggc ggtaaaataa agatactccg cgcgtgcggc atcttctggc gaatttgctg
    14941 cgcaccctgc cagtcgatat cgagaaaaac atcgacaccg gtcgccagta cttgctcaat
    15001 ggcctcacgc gaagtgccat agtaattacc aaaaacttct gcgtgttcga ggaacgcatc
    15061 tctgctaatc atttctttaa attcatcatg attaacaaag aaataatgtt caccgtggac
    15121 ttcaccagga cgcggttggc gtgtggtgtg tgaaacagaa acctgggtgt catacaacgg
    15181 ttgggttttt aataaagcct gaatcaggct ggatttaccc gcgccactgg gggcagaaac
    15241 aatataaagc gtgccttgag ccatgagtat ctttcgtatg tgattagcga aataaagcct
    15301 acatacgagc ttattataca cggcgcggcc atgtgacgta gcccttgctc acactttttg
    15361 cgcaaagttg ttgcgttttg cgtagctgtt tccgcatttg cctgtgattt actgcaataa
    15421 agacgattct caggaagcgt cctcgcagac tccgtttttg ccactcggct ttgccttcac
    15481 cacgttgtat atctgccagc ctgactacag cggaggtgaa gatgaaagta tggatggcaa
    15541 tattaataag tatcttgtgc tggcaatcat ctgtgtgggc ggtctgtccg gcctggtcgc
    15601 cagccagagc acaggaagaa atttcccgcc tgcaacagca aataaaacag tgggacgatg
    15661 actactggaa ggaaggaaaa agtgaggtgg aagacggtgt ttacgatcag ttaagcgccc
    15721 gtcttacgca gtggcaacgc tgctttggga gcgagccccg cgacgtgatg atgccgcctt
    15781 taaatggtgc ggttatgcat ccggttgccc atacgggtgt acgcaaaatg gtggataaaa
    15841 acgcattaag tctgtggatg cgagaacgta gcgatctttg ggtgcagcca aaagttgatg
    15901 gcgttgcggt aaccctggtt tatcgggacg ggaaactgaa caaagcaatc agtcgcggta
    15961 atggcctgaa aggcgaggac tggacgcaga aagttagctt aatttccgct gtgccgcaaa
    16021 ccgttagcgg gcctttagcc aacagtacgc ttcaggggga aatatttctc cagcgcgagg
    16081 ggcatatcca acaacaaatg gggggaataa atgcccgcgc aaaagttgct ggcttgatga
    16141 tgcgccagga cgatagcgac acgctgaatt ctctgggcgt ttttgtctgg gcatggccgg
    16201 atggaccgca gttaatgtct gatcgtttaa aagagctggc taccgcaggt tttactctaa
    16261 cgcagacgta tacccgtgcg gtgaaaaatg ctgatgaagt tgcgcgcgta cgcaatgagt
    16321 ggtggaaagc ggaattaccc ttcgtcaccg atggcgtagt tgtgcgaggg gcgaaagaac
    16381 ccgaatcccg ccattggcta ccaggccagg cagaatggct ggtggcctgg aaatatcaac
    16441 ctgtagctca ggttgccgaa gtgaaggcga ttcagtttgc ggtgggtaag agcggtaaaa
    16501 tatcggtggt tgcgtcactc gcacctgtca tgctggatga taaaaaagtc cagcgggtga
    16561 atattggttc cgtcaggcgc tggcaggagt gggatattgc gcctggtgat cagatcctcg
    16621 tcagccttgc cggtcagggt attcctcgca ttgatgatgt ggtctggcgc ggtgcagaac
    16681 gtacaaaacc gacaccacca gaaaaccgct ttaactcgtt gacctgctac tttgcttctg
    16741 atgtttgtca ggaacagttc atttcacgct tagtctggct ggggtcaaaa caggttctgg
    16801 ggctggatgg cattggtgag gccggctggc gcgcgctgca tcagactcat cgctttgagc
    16861 atatcttttc ctggctttta ttaacgccag agcaattaca gaacacgccg gggatcgcga
    16921 aaagtaaaag tgcgcagcta tggcatcagt ttaatctggc tcgtaagcag ccttttactc
    16981 gctgggtgat ggcaatggga ataccgctaa cccgggcggc gcttaatgcc agtgatgaac
    17041 ggtcctggtc gcaactttta tttagcacgg agcagttctg gcagcagctg ccggggactg
    17101 gatcggggcg cgccagacag gttattgaat ggaaggaaaa tgcgcaaatc aagaagctgg
    17161 gcagttggct ggctgcccag cagatcacag gttttgaacc ttagtgtcct tcgtggctgt
    17221 agtaaaagac cggtaatccc agcttaaggc gtagcgccag taagcgggca aaaaagccga
    17281 ataccagggt ggagataatt accacatcat ggttagaaac atagtgttgc agcgcgatgt
    17341 acagcacggc ggaggcaaat gagacaccgg catacaactc tttctggaat accagtggga
    17401 tacgtttaca gaacatatcg cgcaaaacgc cgccaaacac gccggtggtt accgccgcga
    17461 caacggcaat aattgggccg tgccccatat ccagtgcaac ctgtgcgccg atgatagaaa
    17521 agaccaccag tccaagcgca tcgagcacca gaaacacttt gcgcaggtaa ggcattacag
    17581 gagcaacgat agtggtgagt actgcggcgg tagcgacgat aatcacatat tccgggtgtt
    17641 tgacccagcc gagcggatag tggcccagca gaatatcgcg cactgaccct ccgccaattg
    17701 cggtggcggt cgcaataata attacgccaa atgtatccat gcggcgtcgc ccggccgcca
    17761 gagcccccgt catggcttcg gcagtaatgc caaccaaata caaaatgtgt aacagcatgg
    17821 ttctctccct aaaaatctgt ctggcagggt agcgatttgt gcgtattgtc acgattgaga
    17881 ttttttaagg tgagaggaaa ttgataactt gtagttatgt gaaatcgcta ttttctgtag
    17941 cgtagatgca ttcttctgac ttcttgtctg agaaaatgca taagaaaaaa taggttaaaa
    18001 ctgagggggg aaaagaaagg tgcaatgttt agccattgtg ttcactgcac ctggaccttg
    18061 gcttacgcat cgggtgtctc aatcaacaaa aagagttttt cgtttctgct atttcttctc
    18121 tgtaattttt acactgtttt ccaactgctt gatgctgacc tgcggggtcg gcaactcctc
    18181 aggcatggtt ccgccaagtt cctgaatggt ttgcctcacc ttgctaccca cgtcaaagtg
    18241 tgtggtatta gcttgctgtg ctgcctcaac caattgtttg ttatgttctt tcaactcatt
    18301 tctcagaaac agacgctttt cgtcttcacg aagttgctta aatgcttcat cgtcagcaag
    18361 ctcctgccgt cgggtctgga tagcaaaata agtctgccct gccgcaatga ccggtttcgc
    18421 ggggtcgccg ttttgtacta ccaggtaaca ggcatagcgg gagagtacga tgtcttttaa
    18481 tgctcttttg gcatttgagc caattttgac catatcgagg atctcctcga aatggtcatc
    18541 aattgtgtga ccactgttct cacaggcctc tttggcgcgc gttaatacag gtataaagtg
    18601 acgatactct gaataaccca gtagtttccc aagttgtctt gctgaccatt gttctgctcc
    18661 gtttgcatta atcagtttta tctcttcaaa aggttgatga tgttcgttca ttgcccactc
    18721 catgtgatat cgattgcaaa gaaagtaaat ctgtaactgt atttatatac agttgtgagt
    18781 atctcacgtt ctcagtgtgc tgggcaaaaa aacatcgcaa aatctggagc aggcacgcaa
    18841 aacagaattg aagttgcggg gaagaaaaat gccgctctgt ttgagtgggc ggcatttgtg
    18901 aaggctgaaa agtaagaatt agtgttacga gttactcaat attctgaatc tgctcgcgca
    18961 tctgctcaat caacactttc agctcgatgg cggagtttgt cacttcggca ttgatagatt
    19021 tcgacgcaag agtgttcgac tcgcggttga attcctgcat cataaagtcc agacgacgac
    19081 caacggcttc ttttttcttc agaatgttgt aggtctcttt gacatgcgct tccaggcggt
    19141 ccagttcttc ggcaacgtca attcgttgtg ccagcagaac caactcctgc tccagacggt
    19201 tgttttccaa ctgaacctga gcatcttcca gcttcgcgac cagacgctca cgctgccatt
    19261 gcaggatttc cggcatatgg gcgcggactt tgaccacttc ggcggtgacg ccttccagac
    19321 gctgctcgat caatgctttc agcgcctgac cttcggtttc gcgcgcgacg ataaagtcgt
    19381 ccagggtacc atccaacgcc gcgagaattt cagcggcaat cgcgtcgaga tcctgctcct
    19441 gggctgccat cacgcccggc cagcgtagaa tatcaaccgg gttgatttcc ccttcgtcac
    19501 tctgcatttt tacccagttc gcggcagtta ccagctgttt agccagtttt tcgttgagga
    19561 tcagctcacc ttgtgcgcta acatctggct catagcgcag ggtacattcc actttaccgc
    19621 gcgtcaggcg agaacgaata cgctcgcgaa cgacaggttc aaggctacgg aactgctccg
    19681 gcagacgaaa gtaagtttcc agataacgct ggtttaccga gcgcatttcc caggttgcgc
    19741 tcccccattc acccttgatt tcacgccggg cgtaggcggt catactgcgg atcatagacg
    19801 ttcctgttta taaaaggaga ggtgggagga ttatagccat cgatgccttg tcaggatagg
    19861 aataaccgcc ggaagtccgt ataatgcgca gccacatttg tttcaagccg gagatttcaa
    19921 tatgcgtcca gcaggccgta gcaataatca ggtgcgtccc gttaccctga ctcgtaacta
    19981 tacaaaacat gcagaaggct cggtgctggt cgaatttggc gataccaaag tgttgtgtac
    20041 cgcctctatt gaagaaggcg tgccgcgctt cctgaaaggt cagggccagg gctggatcac
    20101 cgcagagtac ggtatgctgc cgcgttctac ccacacccgt aacgctcgtg aagcagcgaa
    20161 aggtaagcag ggtggacgca caatggaaat ccagcgtctg atcgcccgtg cacttcgcgc
    20221 ggcagtagat ttgaaagcgc tgggtgagtt caccattacg ctggactgcg acgtgcttca
    20281 ggctgatggt ggcacgcgta ccgcttcgat tacgggtgcc tgtgtggcgc tggcagatgc
    20341 gctacagaag ctggtggaaa acggcaagct gaaaaccaat ccaatgaaag ggatggtagc
    20401 cgcggtttct gtcggtattg taaacggtga agcaatttgc gatctggaat acgttgaaga
    20461 ctcagccgca gagaccgaca tgaacgtagt gatgaccgaa gacgggcgca ttattgaagt
    20521 gcaggggacg gcagaaggcg agccgttcac ccatgaagag ttactcacct tgttggcgct
    20581 ggcccgaggg ggaatcgaat ccattgtagc gacgcagaag gcggcgctgg caaactgatt
    20641 tttaaggcga ctgatgagtc gccttttttt tgtctgtaga aaagtaagat gaggagcgaa
    20701 ggcatgaaac catatcagcg ccagtttatt gaatttgcgc ttagcaagca ggtgttaaag
    20761 tttggcgagt ttacgttgaa gtccgggcgc aaaagcccct atttcttcaa cgccgggctg
    20821 tttaataccg ggcgcgatct ggcactgtta ggccgttttt acgctgaagc gttggtggat
    20881 tccggcattg agttcgatct gctgtttggc cctgcttaca aagggatccc gattgccacc
    20941 acaaccgccg tggcgctggc ggagcatcat gacctggacc tgccgtactg ctttaaccgc
    21001 aaagaagcaa aagatcacgg tgaaggcggc aatctggttg gtagcgcgtt acaaggacgc
    21061 gtaatgctgg tagatgatgt gatcaccgcc ggaacggcga ttcgtgagtc gatggaaatt
    21121 attcaggcca atggcgcgac gcttgctggc gtgttgattt cgctcgatcg tcaggaacgg
    21181 gggcgcggcg agatttcggc gattcaggaa gttgagcgtg attacaactg caaagtgatc
    21241 tctatcatca ccctgaaaga cctgattgct tacctggaag agaagccgga aatggcggaa
    21301 catctggcgg cggttaaggc ctatcgcgaa gagttcggcg tttaaaaata tgcgctgtgc
    21361 agggtttttg ctgtgcgcag cgtgatgcgc ttcaagatat cgtgttaatc tgctttcgcc
    21421 agcagtggca atagcgtttc cggccttttg tgccgggagg gtcggcgagt cgctgactta
    21481 acgccagtag tatgtccata tacccaaagt cgcttcattg tacctgagta cgcttcgcgt
    21541 acgtcgcgct gacgcgctca gtacagttac gcgccttcgg gatggtttaa tggtattgcc
    21601 gctgttggcg cctcttttgg ccgccgtgat gtggagagtg gaatggatgc tacccggaca
    21661 acccttctgg cgctcgattt gttcggctcg ccgggctgga gcgtcgataa agaaatacag
    21721 cgactgcatg cgctcagtaa tcatgccgga cgccattacc gacgcattat tctttctaaa
    21781 cgccacggtg gtcagcggct ggtgttagcc cctgattact tgctcaaaac cgtacagcgc
    21841 aacattctta agaacgtcct ttcacaattt ccgctttccc cttttgctac agcctaccga
    21901 ccaggttgcc caatcgtcag caacgcgcag ccacactgcc aacagccgca gatcctgaaa
    21961 ctcgatatcg aaaacttttt cgatagcatt agctggttac aggtctggcg tgtgtttcgc
    22021 caggcccagt tgccacgtaa tgtggtaacc atgctgacct ggatttgttg ttataacgac
    22081 gcgttaccgc agggggcacc aacttcgcca gccatttcca atcttgtgat gcgccgtttt
    22141 gatgaacgca taggggaatg gtgtcaggct cggggaatta cctacacccg ctactgcgat
    22201 gacatgacct tttcaggtca cttcaatgcc cgccaggtta aaaataaagt gtgcggattg
    22261 ttagcggagc tgggcctgag cctcaataaa cgcaaaggct gcctgatagc tgcctgtaag
    22321 cgccagcaag taaccgggat tgttgttaat cacaagccac agcttgcccg tgaagcgcgc
    22381 cgggcgctgc gtcaggaggt gcatttgtgc caaaaatatg gcgttatttc gcatcttagt
    22441 catcgtggtg aacttgatcc ttctggcgat ctccacgcac aggcaacggc gtatctttat
    22501 gctttgcagg gaagaataaa ctggttattg caaatcaacc ctgaggatga ggcctttcaa
    22561 caggcgagag agagtgtaaa gcgaatgctg gttgcatggt aagaaaagcg tcaggcagac
    22621 gtttctgcct gaccgtttag gggagaatta ctgcaactgc gcggcaatta gcggccagcg
    22681 ggcgtcaaaa tcatccgtcg ggcggtattt aaactcgctg cggacaaaac gtgacagcat
    22741 accttcacag aaggccagga tctggcttgc cagcagggtt tcatcggtgg tgtaaccttc
    22801 accctcacgc attctctttt cacgcaatac ctgacgcagc tgcgcttcaa tacgctcgaa
    22861 cagctggttg atgcgccctt gcaggcgatc ctgttcaaac attagcgcat gcccggtaag
    22921 gatgcgggtc aggccaggat tacgctcacc aaaaccgaga agcagcaaca caatcagacg
    22981 caggcgcgct gtggtgtctt tctcatcttt cagaatcagg ttgatgcgag taatcaggct
    23041 atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga agtggcgata
    23101 tagtgccgct tcggaaacgc cgacagaggc agccagtttt gccgtcgtga tacgttggct
    23161 tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc gacggttcct
    23221 tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc acttgccagg
    23281 cggcacccac gctatgaccg caaacgaaat gtttgcggct atgttatgac gttattcgga
    23341 tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg tcggtggcgt
    23401 cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc atctgggcga
    23461 tgcgttcgcc aggttgaatg gtgaagctgt cctgaccacg gttccacacg gaaatcatca
    23521 actggccctg atagtcggaa tcgatcaatc ctaccaggtt accaagcacg ataccgtgct
    23581 tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg gcaatatgaa
    23641 tcgccagccc ggtcggaacc agcgtagtgt cacccggagc cagttctacg gcgtcgtcga
    23701 gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg agcggaaatt
    23761 ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata acgggtcacg
    23821 atctcgtcga gtaataattg gccaaggagc tctttgcgct caagcggtaa gactttatct
    23881 ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg agttggctgg
    23941 gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg ccgggcgtat
    24001 tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg gtcttttagt
    24061 gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgtgaa ttcatcaccc
    24121 tgcgtggcct gctttttgat tttttctggg gccacggtag ctgcgcgata atccgccacg
    24181 gcggcgcaac cgataaaaat attttgctgc tgtacagaag cattcacggc ggcttccatt
    24241 tccagcgcgg tcatcacatc aacacgttta acaaacggtg gtgtcggtag tgaaactgga
    24301 cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg cggcagcgat agcaaaaccc
    24361 atcttgccgg agctgtgatt agagatataa cgcaccggat cgagcggttc acgcgtcgga
    24421 ccggcggtaa tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa atgcgctacc
    24481 gccatatcca caatggttaa cggatcgagc attcgcccag ggccgatatc accacaagcc
    24541 tgactgccac tgtctggccc ccagatgagc aaaccacggg aggcaagcac ctctaaatta
    24601 tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac ggctacacgc
    24661 gcaggtgtag ccagacaaat cgtcgatacc agatcatttg ccattccggc agcaacacgg
    24721 gcaatcaaat ctgccgtggc aggggcgaga atcactaaat cagcccattt acccagctca
    24781 atatggccca tagcggcttc ggctgccggg tccagcagac tgtcggaaac gggataacca
    24841 gaaaccgcct gcaaactaag tggggtgata aaggcttttg ccgcttcggt catggctacg
    24901 cggacgtcgg ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt ataggcagca
    24961 ataccgccgc taacgccgag aacgattttt ttaccggcca ggctcatgat gatttttcct
    25021 gttgggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg tgccttcacc
    25081 tgagattcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat ttaacggaac
    25141 ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat gcacagctgt
    25201 tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgc cttaacggat gtcgagctgc
    25261 tggcgctatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg gcaaaagaga
    25321 tgttggagaa tttcggctct ctttatggct tgttaacctc tgaatatgaa caatttagtg
    25381 gtgttcatgg aattggcgta gcgaaatttg cccagttaaa ggggattgct gaactggcgc
    25441 ggcgttacta caacgtgcgg atgcgtgaag aaagcccttt actcagcccg gagatgacgc
    25501 gggaattttt acaaagccag ctcacgggtg aggagcggga gatctttatg gtgatctttc
    25561 tcgactcaca acaccgggtt ataacgcata gccgactttt ttccggcacg ctaaaccatg
    25621 ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaacgcc tcggcgctga
    25681 tccttgcaca taatcaccct tcgggttgtg ctgaacccag taaagcggat aaactcatta
    25741 ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac catatcgtga
    25801 ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc gctatgcgcg
    25861 atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag cgtatactac
    25921 gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt tcacatagaa
    25981 ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga agctcgagct
    26041 aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc aagcgtccgg
    26101 tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc ctgccgaacc
    26161 tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg cgcgtatctg
    26221 ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct gaactgcgtg
    26281 cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag gtattcgtga
    26341 gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta cgaagaacaa
    26401 acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc gtcagcacgt
    26461 gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga tgtaacaaaa aacctcgctc
    26521 cggcggggtt ttttgttatc tgcttgcccc catattgatt gcatctgttc attcctggag
    26581 atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg cggcatagaa ccgcatctcg
    26641 ttggtgcaac cattcttcat gcagtggtgc gcaacggacg cttgcgctgg ccggtttcag
    26701 aagagatcta ccgtttaagc gaccaaccag tgcttagcgt gcagcggcgg gctaaatatt
    26761 tgctgctgga gctgcctgag ggctggatta tcattcattt ggggatgtct ggcagcctgc
    26821 gcatccttcc agaagaactt ccccctgaaa agcatgacca tgtggatttg gtgatgagca
    26881 acggtaaagt gctgcgctac accgatccgc gccgctttgg tgcctggctg tggaccaaag
    26941 agctggaagg gcataatgtg ctggcccatc ttggaccgga gccgctcagc gacgatttca
    27001 atggtgagta tctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa ccgtggctga
    27061 tggataacaa gctggtggta ggggtaggga atatctatgc cagcgaatca ctgtttgcgg
    27121 cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcagagtgt gaattgttag
    27181 ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggtggtaca acgctgaaag
    27241 attttctgca aagtgatggt aaaccgggct atttcgcgca ggaattgcag gtttacgggc
    27301 gaaaaggcga gccgtgtcgg gtgtgcggta cgccgattgt ggcgactaaa catgcgcagc
    27361 gggcaacgtt ttattgtcgg cagtgccaga agtaattcat gcgcgccgga tggcatacca
    27421 tccggcataa acgctacgct aacttcgcca tcagcgcctg atggacattc tccggcagga
    27481 aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa gagataaacg
    27541 accactcttt cgacggcatc agaaacacac tttccagttc cggcattaag tggcgattca
    27601 tatgcgccag ctgcatttca tattcaaaat ccgccaccgc acgcaggcca cgaatcagca
    27661 ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg accacttcca
    27721 cgttccccag atgcgcggtt gcctgctgtg ccagcgccac acgctcttcc agggtaaaca
    27781 tcggtttttt actggggctg gcggcaatcg ccagaataac gtgatcgaac atttgcgtgg
    27841 cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtacca ggataaatcg
    27901 cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc cagcagttga
    27961 agcagacgct gtagcgcgcc ctggttttga tacagtactt caacggcatg acggccatag
    28021 aaactacggt aatcggcgtc ggtgagtaaa gaggacacct cttttgcaag cgtagtggca
    28081 tcggtaacgg taatcagccc gcttgcctgc tccagccgcg cgcaaatgtc tttaaagtta
    28141 aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg cagcttccag cggattatgc
    28201 ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc gtacagtaac
    28261 atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg gacttcccct
    28321 gaagagcgtg tgatatagct tagtccagcc tggcggacaa gattaatcgc atccgggaag
    28381 cgttctggat gacggggtac caggatgagc aataaattcg ggaattgctg taacaatgcc
    28441 tgatgtgcgg cgatcaccac actctcttcg ccttcgtgag tgctggtggc aatccatacc
    28501 gggcggtgtg gtgcccactg gcggcgcagc gtcacggctt tagcagccaa ctgcggcgtt
    28561 acagaaatat cgaatttcag gctaccggta acggtcacct gattattttt tgcgcccagc
    28621 gccacaaaac gtgcaccatc ttcttcattt tgcgcagcaa tcagcgtaat acgacgcagc
    28681 aagcgacgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc agagagtcgc
    28741 gcgttagcga tcaccagcgg aattttacgt ttgtgtagcg ccgcaatcag gttaggccat
    28801 agttcggttt ccataatcaa caccagtttg gggtcgactt tattcaggaa acggttgagc
    28861 gcatcgggca ggtcatacgg cagataaacg tgctgaacat ccttcccgaa agccgattgt
    28921 acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa tcggtaaatc aggataacga
    28981 tggcgcagcg cgcgcaccaa cgggatcgcc gccagagttt caccgacgga gacggagtgc
    29041 agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc accccagcgt
    29101 tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat cagcggctga
    29161 ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg acttatggat
    29221 gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttcccg tttttgactt
    29281 aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt atttttatgc
    29341 gattcattga tttctgctgt acgtgcagga aggaatcgca gagcaacagt gaaagaatat
    29401 ggttgctaat gattttattt gattgatttc tttattgaaa atcaacaatg tgcttaaaaa
    29461 tcattcgggt cggattgccg cgaaagaagt gatacactag cgcgtcaata tcagtgcggt
    29521 atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg cttcacctta
    29581 atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag aattttgctc
    29641 attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag ttcgctgaaa
    29701 aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat cccgatcctg
    29761 tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca
    29821 gaaaaaattg ccaacttttt tcatctcatc aaggtattac gtgccaataa gtatgacctt
    29881 atcgtcaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt aaatgcccgt
    29941 gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa aagtttcacc
    30001 catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt gctgacacca
    30061 ttgggacttg attcgttggt gaagcagaca accatgagtt acccgcctgc aagctggaaa
    30121 cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt tatccaacct
    30181 acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt gattgatgcc
    30241 ttacatgctc gtggttatga agtcgttctg acgtccggcc cggataaaga cgatctggcc
    30301 tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct ggctggaaag
    30361 gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat tggcgttgat
    30421 tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg
    30481 acagaccata ttttctggcg tccctggtca aataacatga ttcaattctg ggcgggagat
    30541 taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct ttcggttatt
    30601 ccggcggcag atgtcattgc tgctgtcgat aaattactgc cctcctccac gacaggtacg
    30661 tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt ttgcagcgtg
    30721 attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg gtttataccc
    30781 agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg gttaaatcgc
    30841 ataccaatca cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat ttacgcgaac
    30901 atcccgtcga taaagtcgtt ggattcaaca aaatgccggg gctggacgtt tattatgccg
    30961 ctgatgtttg ttatgccgag aaagtagcgc aggaaaaagg ctttttctat cgcctgacgt
    31021 cacgttatcg ccattatgcc gcctttgagc gggcaacctt cgaacagggt aagccgacac
    31081 agttgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat cagactgaag
    31141 cggagcgttt tcatattctg ccaccgggga tttatcctga tcgtaaatat agccagcagc
    31201 cagccaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa caatatttat
    31261 tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg
    31321 cttcgttacc ggattcgctg cgccacaaca cattgctata tgttgttggg caggataaac
    31381 cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt cactttttct
    31441 cggggcgcaa tgatgtctcg gaattaatgg cggcggcgga tttattactg catcctgcct
    31501 accaggaagc ggcgggaatt gtgctgctgg aagcgattac tgcaggatta ccggtactaa
    31561 caacagccgt ttgtggctat gcgcattata ttgtcgacgc caattgcggc gaggctattg
    31621 ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta acgcaatctt
    31681 cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa gatttataca
    31741 gtctgccaga gaaagcggcg gatatcataa cgggtggtct ggatggttga acttaaagag
    31801 ccgtttgcca cgttatggcg cggcaaagat ccttttgagg aagttaaaac cttgcagggt
    31861 gaggtatttc gtgaactgga aactcgccgt actctgcgct ttgaaatggc gggcaaaagc
    31921 tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa tttactctca
    31981 ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg actgcgggat
    32041 gtcggcgttg atactatgta tggggtggca ttcggcgaaa aaggcatgaa tccgctgacc
    32101 agaacctcgt ttattattac cgaagatctg acaccaacca taagtctgga agattactgt
    32161 gctgactggg cgactaaccc tccagatgtt cgcgtaaagc gtatgcttat taagcgtgtc
    32221 gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg ttatatctgt
    32281 catttcctgc tgcacttgcc tttttccggt aaggaagagg agttaaaaat ttcggtaatt
    32341 gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc gttggcggga taaagatctt
    32401 attgggcttt atttttcttc gatgaatatc ggcctgactc agcgggatat ctggcggttt
    32461 atgaaagtgt attttgccgc cccgcttaaa gacattctca agcaggaaca aggactgctg
    32521 tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa
    32581 tttccggaga gaaatatgag tgcccactat tttaatccac aagagatgat cactaagaca
    32641 atcatcttcg atgaaaggcc agcggcgtca gtggcatcat cattccatgt tgcttatggc
    32701 attgataaaa actttctttt tggttgtggt gtttcaatca cgtcagtttt gttacataac
    32761 aacgacgtga gttttgtttt ccacgttttt attgatgata tccctgaagc cgatatccag
    32821 cgtttagccc aattggcgaa aagctatcgt acctgtatcc agatccatct ggtaaattgt
    32881 gaacggctta aggcattacc gacgaccaaa aattggtcta ttgccatgta tttccgtttt
    32941 gtaattgcag attactttat tgatcaacaa gataaggttc tttacctgga tgctgatatc
    33001 gcctgtcagg gaaacttaaa gccgctgata acaatggatc ttgccaataa cattgctgct
    33061 gttgttactg aacgcgatgc taactggtgg tcgttacggg gtcaaagtct gcagtgtaat
    33121 gaacttgaaa agggttactt taattcaggt gtcctgttaa ttaatacact agcgtgggcg
    33181 caggagtccg tttctgctaa agcgatgtcg atgcttgctg ataaagccgt cgtttcccgt
    33241 ttaacctata tggatcaaga tatacttaat cttatcctgt cagggaaag
//
LOCUS       DAPHJE010000042        32806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPHJE010000042 DAPHJE010000000
VERSION     DAPHJE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.042"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..670
                     /gene="zinT"
                     /locus_tag="RH222_002049"
     CDS             20..670
                     /gene="zinT"
                     /locus_tag="RH222_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDV3797070.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1340..1429)
                     /locus_tag="RH222_002050"
     tRNA            complement(1340..1429)
                     /locus_tag="RH222_002050"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1393..1395),aa:Ser,seq:cga)
     gene            1523..2320
                     /gene="mtfA"
                     /locus_tag="RH222_002051"
     CDS             1523..2320
                     /gene="mtfA"
                     /locus_tag="RH222_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV3797071.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2421..2496
                     /locus_tag="RH222_002052"
     tRNA            2421..2496
                     /locus_tag="RH222_002052"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2454..2456,aa:Asn,seq:gtt)
     gene            2658..3573
                     /locus_tag="RH222_002053"
                     /pseudo
     CDS             2658..3573
                     /locus_tag="RH222_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3767..5071)
                     /gene="ybtS"
                     /locus_tag="RH222_002054"
     CDS             complement(3767..5071)
                     /gene="ybtS"
                     /locus_tag="RH222_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HDV3797072.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5099..6379)
                     /gene="ybtX"
                     /locus_tag="RH222_002055"
     CDS             complement(5099..6379)
                     /gene="ybtX"
                     /locus_tag="RH222_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HDV3797073.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6372..8174)
                     /gene="ybtQ"
                     /locus_tag="RH222_002056"
     CDS             complement(6372..8174)
                     /gene="ybtQ"
                     /locus_tag="RH222_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HDV3797074.1"
                     /translation="MKDNNPADNLAWRVIWRLLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(8161..9873)
                     /gene="ybtP"
                     /locus_tag="RH222_002057"
     CDS             complement(8161..9873)
                     /gene="ybtP"
                     /locus_tag="RH222_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HDV3797075.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYVRAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            10130..11089
                     /gene="ybtA"
                     /locus_tag="RH222_002058"
     CDS             10130..11089
                     /gene="ybtA"
                     /locus_tag="RH222_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HDV3797076.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            11280..17387
                     /gene="irp2"
                     /locus_tag="RH222_002059"
     CDS             11280..17387
                     /gene="irp2"
                     /locus_tag="RH222_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HDV3797077.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     SAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTDNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSLEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHLFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            17475..26966
                     /gene="irp1"
                     /locus_tag="RH222_002060"
     CDS             17475..26966
                     /gene="irp1"
                     /locus_tag="RH222_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HDV3797078.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWASEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGTRVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNKAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTSWETLHQGLSAVSLSQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESNGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLANIAHLLRLLTEHTPGESVPVPCLMVYAAGRPSRWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            26963..28063
                     /gene="ybtU"
                     /locus_tag="RH222_002061"
     CDS             26963..28063
                     /gene="ybtU"
                     /locus_tag="RH222_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HDV3797079.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSNLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLADEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            28060..28863
                     /gene="ybtT"
                     /locus_tag="RH222_002062"
     CDS             28060..28863
                     /gene="ybtT"
                     /locus_tag="RH222_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HDV3797080.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            28867..30444
                     /gene="ybtE"
                     /locus_tag="RH222_002063"
     CDS             28867..30444
                     /gene="ybtE"
                     /locus_tag="RH222_002063"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HDV3797081.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            30575..32596
                     /gene="fyuA"
                     /locus_tag="RH222_002064"
     CDS             30575..32596
                     /gene="fyuA"
                     /locus_tag="RH222_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HDV3797082.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         7166 a         9757 c         8992 g         6891 t
ORIGIN      
        1 aactctggag gaaactgttt tggcgattcg tcttcataaa ctggctgttg ctttaggtgt
       61 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
      121 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
      181 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
      241 tgaccccatc tttcagaaga aagcggatgc agataaaacc aaaacatttg ctgaaattaa
      301 agattattat cgcaaaggtt atgtgacaga catcgagatg attggcattg aggacggcat
      361 tgtcgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
      421 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
      481 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
      541 atctcatttt cacattttta tgggtaatga ttcccaacag tctttattga atgaaatgga
      601 aaactggcca acgtattatc cctatcagtt gagtagcgaa gaagtggtcg aggagatgat
      661 gtctcattga tttttttgcg ctgacttcag ttatttaaca gaacagtgcc tggtagtaac
      721 aacaataatt tgccaagaca aataagtaag acaaactcag acacgctcat acttgagtcg
      781 tgtctttttt attatgtctg tagaaaaaaa atgcgaaaaa ggcgaacaat aaatacacaa
      841 ctcagccata cagactggca gcgcaaatgt ttccaggagc aagtcatctg tcatggtaaa
      901 gctaaccaga aatataggtt aattttggtg tttaaacctt ttttacttgt tagtatgata
      961 caaaggcttt caaaaaagct gggaaattca acaactcttg gaaattatgg actttgtccc
     1021 gcggctttaa tctgacggcc gcgttccttt tttgactcaa ttattgaccc cttctctacg
     1081 caacttcagt tcccgccagc aactttgcgg cagtttcata ggatcaatgt ctaaaagaat
     1141 aatggtgacc gataagaaaa cgactgaata actgtagatt ttcgctcgaa accttcctgt
     1201 cagctccata gcgaatcaag tgctgaatgt cacagtatcg aacagaaaac agtgacgatc
     1261 taacccttca agaatattct acgattgttc tgtttaggaa aagcaaggcg ggaagtcagg
     1321 agataagtca ttgataaagt ggcggagaga gggggatttg aacccccggt agagttgccc
     1381 ctactccggt tttcgagacc ggtccgttca gccgctccgg catctctccg ttcagatggt
     1441 tgccatgatg ccaggaaatt tggcatttta acagtccctg tccgtgcaat tttgttcaag
     1501 tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg aaagtacaag aatcagcaca
     1561 tcaaactgcc cttccctggc aggaagcact atcgatcccc cttttaacgg gtctgacgga
     1621 acaggaacaa agcaaattag tcactcttgc cgaacgtttt ttacagcaaa agcggcttgt
     1681 tcctttacag ggctttgaac tggattcatt aagaagctgc cggatagcac ttctattttg
     1741 cctacccgtt ctggagttag gactggaatg gctggatggt tttcatgaag tcttaattta
     1801 tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat atcggtctgg tgcataacca
     1861 acgtattgtt cagtcaggtc agagctggca gcaagggcct atcgttttga actggttgga
     1921 tatacaagat tcttttgatg catctggttt taacctgatt attcatgaag tcgctcataa
     1981 gctggacacc cgtaacggcg atcgcgccag cggagttccc tttatttcgt tgcgtgaggt
     2041 tgctggctgg gaacacgatc ttcatgctgc aatgaacaac attcaggaag aaatcgaatt
     2101 agttggtgag aatgcagcga gcattgatgc ttatgctgcc agtgatcctg ctgaatgttt
     2161 tgccgtactt tctgaatatt tctttagcgc cccagaactt tttgctcctc gtttcccttc
     2221 attgtggcaa cgtttctgtc aattttatca acaagatcct ttgcagagac tgcatcacgc
     2281 taatgataca gactcgtttt cggcgacgaa tgttcattaa ttaacaactt tgcagattaa
     2341 tcaaccaatt gaaatggctt atgaaattta gtgttgacag acaaggtacc gctaagtaat
     2401 atgcgctccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     2461 cgtatgtcac tggttcgagt ccagtcagag gagccaattt tctgttttca tgcatccttg
     2521 caaatcttta tctgatgttg attcaacagg ttagtgtgaa aactcttctc ggtgcattct
     2581 gattttaccc tacgcatcgg gaaaaattgg tggtcaaatc tgggatcagg ttagttcgat
     2641 aatggagtga cccccatatg ttccttaccg acgcaaaaat ccgcaccctc aagccttctg
     2701 ataaaccctt taaagtctcc gattctcacg gtctgtatct gctggtcaag ccgggtggct
     2761 cccgccactg gtatctcaaa gctggaggtt gcgtcccgca cacggcagca cctgagtaac
     2821 ataatgcgcc atgcggtcca tcaggagtta atcgatacga accctgcagc aaaccttggc
     2881 ggcgtgacca cacctcctgt cagacggcac tatcctgccc tgccgctgga gcggctgcct
     2941 gaactgcttg aacgtattgg ggcatatcat cagggccgtg aactgacccg gcatgccgtt
     3001 ctgctgatgc tgcatgtgtt cattcgctcc agtgaactgc gtttcgcccg ctggtcagag
     3061 attgatttca caaaccgagt ctggacgata cccgcgacgc gagaacccat tattggcgtg
     3121 cgttattccg gccgcggggc aaaaatgcga atgccgcata tcgtccccct ctcagaacag
     3181 tccatcgcca ttctgaaaca gattaaggat atcaccggta ataatgaact gatcttcccc
     3241 ggcgaccata acccgtataa gccaatgtgt gaaaacacgg tcaataaggc actgcgggtg
     3301 atgggttacg acacgaaaaa ggatatctgc ggtcacggct tccgggcaat ggcatgcagt
     3361 gcgctgatgg aatcgggttt atgggcaaag gacgcagtag aacgccagat gagtcatcag
     3421 gagcgcaata ccgtgcgcat ggcttatatt cataaggcag agcacctaga agcccgcaaa
     3481 gcgatgatgc agtggtggtc ggattatctg gaagcatgcc gagaatctta tgcaccgcct
     3541 tatacaattg gtaaaaataa gtttatccca tagtatacta acgaatccct cactctggca
     3601 aattaccacc acctccacag gaggtggttc tatatagact tttatcaact tcatgacgaa
     3661 aaacacctat tttatcccaa aagaagacaa tagagtcaca gaggaatcaa agagcgactc
     3721 aattgtcttt ggacagtctg gttgtgaggt tagaaaacag ttactcctac accattaaat
     3781 agggcgcaat gctcgctaat ttctcccggg tttctgtcag ttcgcgttcc ggcgtagatt
     3841 gcgcgatgat tcccgccccc gcctgtatcc agcagcgctg gctatcctga aatacggaac
     3901 gcagaactag cgccgcatca aagcgcgtat cgtccagcag caggattgcg ccggaataaa
     3961 gctctcgcgg cgtcttctca atttgcataa tcgcgttcag agcagcattt ttagggatac
     4021 ctgaggcggt aatcgacgga aacagcacgg taaacgcatc ccaggcatcc ttgttttccg
     4081 aaagctgacc gctcacgcca gaccccagat gctgaacgct gccgcgctgg cgaaccgaca
     4141 ttaaatcttc aaccaccacg ctgcccggct ggcaaacagc ctccagttca gcaatagctt
     4201 ctttgacaga aaggatatgc tcaagcacct ctttactgtc gtgcagcagt tctgcctctt
     4261 tcgccttatt atgctccggg tttcccatgc gatcgcgggt gcccgcaagc ggttcagtga
     4321 ccactttatt gcccatcact gacatcacca gttccgggct aaagcccagc gcttcgcgtc
     4381 cttcctgacg gaacataaac gagcgcacag gcgtgtttgc ctgccgcccg tataacagcg
     4441 tggcgggcat atcaatccgc gatggcaggg gaatggcgcg cgagacaatc actttgacat
     4501 actcgccacg gcggatctcc gcaacggcgc gcgcaacctg ttgtttatat gcctcaccat
     4561 tgagggcggt atccaccgcc agtggtgcgt tctgcgtcgt tgtaccggcc tcttttaccc
     4621 actcgcacaa acgtcggcac ccgtcggcgg agtccgcata aacggtgaca tttccctttt
     4681 caaaaataag ttcttcacgg ggaacggtta acgtcagcag cggccactcc ccggcgttaa
     4741 aggcaatccc ccgcgcatgc gccgcaaagt tgaacccaac ctgtccatat acccgacgtc
     4801 ctttcacctg aggatgcgcc ataaaacgac gcgcgcagtc ggcaatggaa tccaccgccc
     4861 atttttgctc cccggcatca tcaataaatg tcgcctgcgt tccgtcggca ttaatgtgca
     4921 gccgtgcctg gcaccctttg cctaaatacc aacagggttg acgctcatag acataacact
     4981 cttcttctgc gaactggcgt aaaacgccag aaatcgtcgg tagccattgt tcctctggta
     5041 acgccagatg tagaaattca ctgattttca tcccttacct ctctgtgtta ttcccggatc
     5101 aatcaattgc gcttgtcatc agttctcttg ccgaatgcag ccggatataa gcaacccacg
     5161 ccgccagcaa cgtgaatgcc ccggcaaaca ggaagcaggc cgcatagcca aaatgttgag
     5221 caacaacgcc accgataacg cctgccagaa tggcgattgc cgcatcagta cattgaaaga
     5281 gggtgaagtc gacaccggcc tgcaaaggcg atgaaagcga catcagcgtg gcgtaaagac
     5341 agaccagcgc gcaggccagc gaaatggact ggacaatgac cagacactgg agaatctgca
     5401 gcagcagatg acccggcgcc atcatgagcg tcatcaccac cgccagcagc gcgatcccct
     5461 ggacgccata agccgtcaac agcgctctgc cgggtgaggt gtatttcatc agcaatccgc
     5521 cggccagcgt tcctgctatg cccgctgcaa tattgccgcc gctgaacaac gcgcccaatg
     5581 cggacatgct caacccatga tccaacaaca gcggtgccag aagaggcagc acaaaccgca
     5641 tgcctgaatt cagcatcaat accagcagta agcccaggcg cgcctgcttc ctccttagcg
     5701 cataacctaa cgccggaaca tgcaggatag tcgctgttcg cgtcggctcc gtaatgcgcc
     5761 acagcgggag tgacagcgcc ataatcagca ccgccatcat cagcatggcg acaggccagc
     5821 cggatgccgc cgacagcatc aggaacaccc cgccgccgca catcattccc agatagctgc
     5881 cgccgacctg cacgctgttt ccccagccgt aacccgtgcg ggtcagttga tcgacacaaa
     5941 agccgtcgct ggcgatatca atggtggagg cgaccgtgcc tgccaccata aacagaacaa
     6001 ataacgcgac gatttgcgta tcggggaatc ccccttctcg cctaaaccag ccaatcgctg
     6061 cggcaatagt caggatcgcc gctagcgcga cctgaccacg caggattaac atgcgggaac
     6121 ggcgttcctg gctaccgggc ggaagccgcc agcgctcgat ccacggcgcc caaataaact
     6181 tcagcgccca gggcagcata aacagggttg tcgcaccggc aagcgccagc gaaccgccag
     6241 cagcgcgcac cagcgcgggt aaggactgca tagacagcgc cgataccaga ctctgcgtca
     6301 catagacgcc ggcaatagca aaaatcaccc gcccggtcgt caacgtcagc ggtttcacat
     6361 tcgactgaac atcactcatg cacccactct ccagacgcgg aaaccccgtc gtcgcgccac
     6421 acgcgcgcgg ccatttgcgc ctgccacagc gcctgataac gcccatgatg tgagagcaat
     6481 tgcgcatgag taccctgctc aaccacctgt ccctcttcca tcaccagaat gtttccggcc
     6541 ccggcgatgg tggataaacg gtgagcgatg ataatcaccg tccggttgta aaccaggtta
     6601 tcgatcgctt tttgcaccgc cagctcgctt tcaatatcca gcgcggcagt cggttcatcg
     6661 agaatgacga ctggcgcgtt tttcaataac gctctggcaa tggaaatccg ctggcgctcg
     6721 ccgcccgata gctggccgcc catctctccc attggcgtca gccagccttg cggaagacgg
     6781 gaaataaact caaggcactg cgccgcgcgg gcggcctctt ctacctcctg ccgcgtcgcc
     6841 tgtgggcgcg cgatacggat attcgccagc agcgtgtcat caaacagcca gacatcctgg
     6901 aaaacaacag aaatcaggct attgagctgt tccggcgtca ggcggcgaat atcgacgccg
     6961 ccaatagaaa tctgcccttg ctgcggatcg gcatagcgca ttaacagttt ggtgacggta
     7021 gttttgcctg cgccggaggc acccaccagc gcgctcatac tggctgccgg gaacgtcaaa
     7081 gaaacatgat taagcgcgtg gccgtcgcct tcttcgtagc gatagctgac gttgtcaaag
     7141 cggatatcgt aacgttccgg catctcgctt tgctctgcga cgggaagcgg tgctatcgcc
     7201 ataaactgct caatccgttg cagggcgctg gcgatcagtt ccacaaccga ggtgtagctg
     7261 ataaacatcg ccatcggttc tgcgaagcgc ataatcatcg cgacggcggc aatcaaaaag
     7321 gcgaggttca gagtgcccgt cactacccag acgatcccgg ataacaccac cacctgtagg
     7381 cccaactcca cgacgctggc gatcagcatc gtcgcgccag cgccctgacg gtgagtgcgg
     7441 gtctgtaagt tttccagcgc attgaaatgc gccagcagcg cccggctttt atcggcatcg
     7501 ctgccgcagg tgcgcaatac catcatcccc tgagcaaatt caacgatatc gccgctcaga
     7561 cgctggtgcg cttcccccag cgtctgcatt tgtcgccgca tcgccgggcg gcgccagtaa
     7621 taaaacggca ccagcagagg gaagatcagc aacatcacca gccccagccg ccagtctatc
     7681 cacaatgtcg ccagcgacgc cgtcagcggc gtgacaatgg tgagcagcaa aatattggct
     7741 atcgcaataa cataattgag gttttcatcc acgctgccca gcagcaaggc gttcatttca
     7801 cccgccctgc cgcgctggag cttctccagc ggcacgcggc gtaactgctc gccaagtcgc
     7861 aggcgcaact catgggtggc ctgcgccaga tgaccacggt attcaaagcc caggccatac
     7921 cagcgtagca ccagcgtcac aattgcggcg acgctgaagg ccatagccca attaagaagt
     7981 tgcggcgcgt ctccccgtaa cagcgcatca atgatcggat aaagacaggc aaaggcgatc
     8041 ccctgcatga atgccgccag caacagcgcc agcatactgc gtcgcagcat cctggcctga
     8101 ctgccaacgc tggagataag caggcgccag atgacgcgcc aggccaggtt atccgcagga
     8161 ttattgtctt tcattttcca ccacctcttc ctgtgttcca cccggcaccc aatgctgcgc
     8221 ctgctgatag tgttgccaga gccgttgata caggccgccc tgcgccaaca gttgcgtatg
     8281 gttccccatt tccctgagct gtccgtcgga aaacagcaat atcacatcgg cctgagtcac
     8341 cattgagaga cgatgcgcga ccatgatgac cgtccggcca cgcatggccg ccgcgagcgc
     8401 cttgataagc gccgcttcgt tttccgggtc agcaaacgcc gtcgcctcat cgagcaccag
     8461 gatggggcga tcctgcaaaa gcgcccgggc gatagtaatg cgctgccgct ggccgccgga
     8521 gagaaatacc ccacgttccc cgactcgagt gttgtaacct tctggcagag cgctaataaa
     8581 atcatgggcc tgcgccactc tggccgccgc tattaccgcc tccagcggcg tatccggcgc
     8641 gcccagacga atgttattgg ctatcgtgtc gacaaaaagg aagttgtcct gaaacacaaa
     8701 cgagagttgc ttcatcaggg tgtccgtctg catatcacgc agatccacgc cgccaatacg
     8761 gatatggcct ttgtccgggt cggcgtaacg cagcagcaag cgcgccacgg tgctttttcc
     8821 ggcaccgctt ggcccgacca gcgccacaat ttgcccggca ggcacatgaa agctcacctc
     8881 ctgcaacgcg gcgccagtac gcgcttgcgg ataatgaaag ctcacctgct caaaggtaat
     8941 gctcgcctct tgcggctgct ggtcagactg cggcagcgat aactccggca tcgcgagaac
     9001 ctgataaata cgctgaatgc ttaaacgcgt ttgcgcgacc aggttattga gcatcatcat
     9061 tggcattacg gcttcggcca ttccgctgcc cagcagtaaa acggccaccc acgcgataaa
     9121 atcgaaactg ccatagtgca acaggccgta tcccgaccag atcaggacaa acagggtcgg
     9181 gagaggattc agaatcgaga aggaaaaacg cgctgaaaaa ccggctttgc gataccaggt
     9241 tttgagcaca tcgacccact cttcaagggc gcgttgatag cgcaaaaaac tggtgctgcc
     9301 gctatcaaac gttctcacca cgggcatggc ctgcacgaac tcaataaccg ccgcgctaac
     9361 ctgctcgcgc gccttatgat aacgctgagc catatcttcc gaacgctggc gggcgagcac
     9421 gagaacaacc gatccaaacg ccagtacccc cagcgtcgcg attgccagcc gccagtccag
     9481 ccagaacaag ataacgattg tcgccagcgg catgatgatc gcgcggacat agagcggcgt
     9541 gctgtcggcc acaaaaatat gtaacgactt cacgtcatcc agcatcactt tcgccactga
     9601 cccgctgccc atttgctgta acacgcctgg cggaagctgc aatgctttac gggctaacct
     9661 gctgcgcaga atgttctcca gatgaaaagc cgcgtaatga gaggtgttga acgcctgtaa
     9721 gcgcagaaca aaagttaaga ccacgcagcc gatcacgccg cccagcggcc aggccggaat
     9781 agcgtcaggc gtcgcgcgaa tgtcacgcag gctccacgcc agaaaaagga gcgcccccag
     9841 gctggttaat gccgccagcc ccgccagcgc catagctaaa ataaccttcc tgcgaaccgg
     9901 tgtaatcacc tgccagagag gaaatcggga cagcgattca gtattggatg attgagatga
     9961 catgggagta actgaatttc ctgatgaatt tttacggacg ccaaaaacga caattcaggg
    10021 tggcgttctg agaattaatg tgaataataa ccattatcaa taactcatct accccattcg
    10081 gttctattta tacccgcatt ggtctaagcc acagggagat aaccaggtca tgatggagtc
    10141 accgcaaacg caatctgaaa tctctattca ccagttggtg gtcgggaaac ctgcaaacga
    10201 tggcaatatt cctgctcagt gtgaactgct gcgctgttcg ttgcaggaag gtatggatat
    10261 tttgctctgg aggggccatt ttgcgcggcc tgagacgttg cagctacacg acgatctggg
    10321 caggattaac ttctcgtgca tcctggaagg cacatcgcgc tttgcgattc agggactccg
    10381 gcgccacacc gactgggagc ttgcgcgcaa tcgtcattac attacccata cgccagactg
    10441 tcgcggcagc gcctcctatt gtggccgatt cgagagcatt accctctcct tcagccctga
    10501 aaccctcgcg ctgtgggtgc cggatattag cgcggtcatt aagaataaga tcgactccca
    10561 ctgctgttgc cagcagcagc gttgtaatgc tgaaacccac ttaacggctc aggccttgcg
    10621 tcacgcgctg atgcgcatgc acggcggctt cagtcatgaa caaaagccgt cgacgctatg
    10681 gttgcagggg caaagtctgg tcatgctcag tctggtgctg gatgaacatc gcgaagacgc
    10741 cagttgcctc tcctgccact tcaacccgat ggaacgccag aaactgctgc gggcgaagga
    10801 tctgctgctg gctgatctga cgcaagcgcc gggcgtcgcg gagctggcca gagagtccgg
    10861 tctgagcgtt ctcaaaatta agcgaggctt ccgcgttctg ttcaacaaca gcgtctatgg
    10921 cctgtttcag gctgaacgaa tgcaggaagc cagaagacgc ctggccaacg gcaatacctc
    10981 cgtcatgacg gtggcggccg atctgggcta tgccaacgcc agccatttct ctgccgcctt
    11041 ccagaaacag tttggcgtaa ctccttcgac ctttaaacgc gggatgtaac cggggtcgcg
    11101 cccccctaaa ctcctccctg acagaggccc ggatacccca tccgggcctc tgttctttcc
    11161 gcctgttacc aacccgaaac gggcataaat aaacccgttt cgggtagcat ccaattgtta
    11221 ataattatta ttctcatatg agtaatgctt ttcggtaaga cgtgccatca ggaggaagaa
    11281 tgatttctgg cgcaccatct caggattcgc tgttaccgga caaccgccac gcggctgatt
    11341 accaacaatt acgcgagcgg ctcatacagg aactgaattt aacgccgcag cagttacatg
    11401 aagagagcaa cctgatccag gccggtctgg attccataag attgatgaga tggttacact
    11461 ggtttcgtaa aaatggctac cgccttaccc ttcgcgagct gtatgccgcc cccacgctgg
    11521 cggcatggaa ccagttaatg ctcagccggt cgccggagaa cgcggaagaa gaaacgccgc
    11581 ccgacgaatc atcctggccg aacatgaccg aaagtacccc cttcccattg acgccagtac
    11641 agcacgccta cctgacgggc cgcatgccgg ggcagacgct tggcggcgtg ggttgccacc
    11701 tgtatcagga gtttgaaggc cattgtctga cggcgtcgca gctggagcag gccatcacga
    11761 ccttgctgca acgccaccca atgctgcata tcgcctttcg ccccgacggg cagcaggtct
    11821 ggctaccgca accttactgg aacggcgtca ccgttcatga tttacgccat aacgacgctg
    11881 aaagccgcca ggcctatctg gacgcactgc gccagcgcct gagccaccgt cttttacgcg
    11941 tggaaatcgg cgaaacgttt gattttcagc tgacgctctt gccggacaat cgccaccgcc
    12001 tccatgtcaa tattgacctg ctgattatgg atgcctccag ctttacgctt ttcttcgatg
    12061 agcttaacgc cctgctggcc ggagaatcgc tgccggctat cgacacccgc tatgatttcc
    12121 gctcgtattt gctgcaccag cagaagatca atcaaccact gagagacgtc gcgcgcgctt
    12181 actggctggc gaaagcatcg acgcttcccc ccgcgcccgt cttgccgctg gcctgcgaac
    12241 ccgccacgct acgtgaagtc cgtaataccc gacgccgcat gattgtcccg gcaacacgct
    12301 ggcacgcctt tagcaaccgg gccggcgagt atggcgtgac gccgacaatg gcactggcga
    12361 cctgtttttc tgccgtgctg gctcgctggg gcggcctgac gcgtctgctg cttaacatca
    12421 ccttattcga ccgccagccg ctgcaaccgg cggttagcgc gatgcttgcc gacttcacca
    12481 atattcttct gctggatacc gcctgcgatg gcgataccgt cagcaacctg gcgcgtaaaa
    12541 accagctcac gtttacggag gactgggagc atcgccactg gtccggcgtc gaattactcc
    12601 gtgaactcaa acgccagcag cgctaccccc acggcgcccc ggtggtattt accagcaatc
    12661 tggggcgttc cctctacagc agccgcgcag aatcgccgtt gggcgagccg gaatggggca
    12721 tctcgcaaac gccgcaggtc tggatagatc atctggcgtt cgagcatcac ggcgaggtct
    12781 ggctacaatg ggacagcaac gacgcgctgt tccctccggc gttagtcgaa acattgttcg
    12841 acgcctactg ccagttgatt aaccaactct gcgatgacga aagcgcctgg caaaagccgt
    12901 tcgcagatat gatgcccgcc agccagcgcg cgatacgcga acgggtcaac gccaccggcg
    12961 cccccattcc cgaaggcttg ctgcatgaag gcattttccg tatcgctctg caacagccgc
    13021 aggcgctggc ggtaacggac atgcgttatc agtggaatta tcatgagctg acagactatg
    13081 cccgccgttg cgcgggcagg ttaatcgagt gcggggttca gcccggcgat aatgtggcta
    13141 tcacgatgtc gaaaggcgca ggacaacttg ttgcggttct ggccgtcctg ctggccgggg
    13201 cggtttacgt tccggtttcg ctggatcagc ctgccgcacg gcgcgagaaa atctacgctg
    13261 acgccagcgt ccggctggtg ctcatttgtc agcacgacgc cagcgccggg tcagacgata
    13321 ttcccgccct tgcctggcag caggccattg aggcggagcc gatcgccaac ccggtagtac
    13381 gcgcccccac gcaaccggcc tacattatct acacctccgg ctctaccggt acgccgaaag
    13441 gggtagtcat ttctcaccgg ggagcgctta acacctgttg cgatatcaat acccgctatc
    13501 aggttggccc gcatgacagg gtgctggccc tctccgccct acattttgat ttatcggttt
    13561 acgacatttt tggcgtactg cgcgcgggcg gcgcgctggt gatggtgatg gaaaatcaac
    13621 ggcgcgatcc tcacgcatgg tgtgagctga tccagcgcca tcaggtcacg ctctggaaca
    13681 gcgtcccggc gctgttcgat atgctgctga cctggtgtga aggtttcgcc gacgccacgc
    13741 cggaaaacct gcgcgcagtg atgctttccg gcgactggat cgggcttgac ctccccgccc
    13801 gttatcgggc cttccggcca caaggacaat ttatcgcgat gggcggcgcc accgaggcgt
    13861 ctatctggtc taacgcctgc gaaattcacg acgtccccgc ccactggcgc tccatccctt
    13921 acggttttcc gctaaccaac caacgctacc gggtggtgga tgaacagggc cgggactgcc
    13981 ctgactgggt gccgggtgaa ttatggattg gcggcattgg ggtcgcggaa ggctatttca
    14041 acgatcccct gcgtagcgag cagcaatttt tgacgctccc ggacgagcgc tggtatcgca
    14101 ccggcgatct cggctgctac tggccagatg gcacaatcga gttcctcggt cgtcgcgaca
    14161 agcaggtcaa agtcggagga tatcgcatcg agctgggcga aatcgaaagc gcgctcagcc
    14221 agctggtggg ggtgaaacaa gcaaccgttc tggcgatcgg cgaaaaagaa aaaacgctgg
    14281 cggcatacgt tgttcctcag ggcgaggctt tttgcgttac cgatcatcgg aacccggcac
    14341 tgccgcaggc gtggcacacg cttgcgggaa cgttgccctg ttgcgccatc tcgccagaga
    14401 tctccgcaga acaggtagcc gatttccttc agcatcgcct gctaaaactg aagccgggtc
    14461 acaccgctgg cgccgatcct ctccccctga tgaactcact cgctatccag ccgcgctggc
    14521 aggccgtggt ggaacgctgg ttagcatttc tggtgacaca acggcgactg aagcccgctg
    14581 ctgaaggtta tcaggtctgc gctggtgaag aacgcgagga tgagcacccg cacttcagcg
    14641 gacatgattt aacgttatcg caaattcttc gcggtgcccg taacgaactg tcgttactga
    14701 acgacgcgca gtggtcgccg gaaagcctgg cctttaacca tccggccagc gccccgtata
    14761 ttcaggaact ggcgacaatt tgccaacagc ttgcacagcg cttacagcgc ccggtacgcc
    14821 tgcttgaggt gggaacccgc actggccgcg ccgcagaatc gctgttagca cagctcaacg
    14881 ccggacagat tgagtatgtc gggcttgagc agagccagga gatgctgctg agcgcccggc
    14941 agaggctcgc cccctggcct ggcgcccgtc tgtccctctg gaatgcagac acgctggcgg
    15001 cgcacgctca ctcggcggac attatctggc ttaataacgc cctgcatcgt ctgctgccgg
    15061 aagatcccgg gctccttgcg acattacaac agcttgccgt tcccggcgcg ctgctctacg
    15121 tgatggagtt tcgccagtta acgccgtccg ccctactcag cacgctcctg ttaaccaatg
    15181 ggcagccgga ggccttgctg cataacagcg ccgactgggc ggcattattt agcgcggccg
    15241 gcttcaactg tcagcatggc gatgaggtcg cggggttaca acgcttcctc gtacaatgtc
    15301 ctgacaggca ggtgcgccgc gatccccgtc aacttcaggc cgccctcgcc gggcgtctgc
    15361 cggggtggat ggtgccgcaa cggatcgtct tcctcgacgc cttaccgctg acagctaacg
    15421 ggaaaattga ctaccaggcg ctgaagcgtc gtcatacccc tgaagcggaa aacccggccg
    15481 aagcggattt accccagggc gacattgaaa aacaggttgc cgccctctgg cagcaactct
    15541 tatcaactga caatgtcacc agagaaaccg acttcttcca gcaaggcggc gatagcctgc
    15601 tggcgacccg tctgaccggg caacttcatc aggcaggtta tgaagcgcaa ttaagcgacc
    15661 tgtttaatca tccccggctg gcggattttg ccgccacgct gcggaaaacc gacgtcccgg
    15721 tcgaacaacc attcgtccac tcccttgaag atcgctacca gccctttgcg cttaccgacg
    15781 tgcagcaggc ttacctggtg gggcgtcagc cgggctttgc cctgggcggc gtcggctcac
    15841 atttatttgt tgaatttgaa attgccgatc tggacctcac ccggctggag acggtctgga
    15901 accgattaat tgcccgccac gatatgctgc gcgccatcgt gcgtgatgga cagcaacagg
    15961 tgctcgaaca gacgcccccc tgggtgatac ccgcacacac cctccatacg cctgaagagg
    16021 cgttgcgggt gcgcgaaaaa ctggcgcatc aggtactcaa ccccgaagtg tggccggtat
    16081 tcgatctcca ggtcggatac gtggacggga tgcctgcccg cctgtggctg tgtctggata
    16141 acctgttgct tgacggtctg agcatgcaga tcctgctggc ggagctggag cacggctacc
    16201 gctacccgca acagctgctt ccgccgctgc ccgtcacctt cagggattat ctgcaacaac
    16261 cctcgctaca gtcgcccaat ccagattctc tggcatggtg gcaggcgcag cttgatgata
    16321 ttcctccggc gcctgcgttg ccgctgcgct gcttgcctca ggaggttgaa acaccgcgct
    16381 tcgcccgcct gaacggcgca ctggacagca cgcgctggca tcggctgaaa aaacgggcgg
    16441 ctgacgccca tctcaccccg tcggccgtac tgttgtcggt gtggtcaacg gttctctctg
    16501 catggagtgc acagcctgac ttcacgctta accttacgct tttcgacagg cgaccgctgc
    16561 acccgcaaat caaccagatt ctgggcgatt tcacctcgct gatgctgctg agctggcatc
    16621 ccggcgaaag ctggctgcac agcgcgcagt cactacagca gcggctgagc cagaacctca
    16681 accaccgcga tgtgtcagcc atccgcgtga tgcgtcaact ggcgcaacgg caaaacgtgc
    16741 cagccgttcc gatgcccgtc gtctttacca gcgcgctggg ctttgagcag gataacttcc
    16801 tcgcccggcg taatctgctc aaaccggtct ggggcatctc ccagacgccg caggtctggc
    16861 tcgatcacca gatttatgaa tccgaaggcg aactgcgctt taactgggat tttgtcgccg
    16921 cgctgtttcc tgccgggcag gtggagcgcc agtttgaaca gtattgcgca ttgctaaacc
    16981 gaatggccga ggatgaaagc ggctggcaac tgccgctcgc cgcgctggtg cctcccgtta
    17041 aacacgcagg gcaatgcgca gagcgctcac cgcgcgtatg ccctgagcac tctcagccac
    17101 acattgcggc ggacgagagc accgtcagcc tgatttgcga cgccttccgc gaggtggttg
    17161 gcgagtctgt cacgcccgca gaaaacttct ttgaggcggg cgcaacgtcg ctgaatctgg
    17221 tgcaactgca cgttttgtta caacgtcacg aattttccac cctgacgttg cttgacctct
    17281 tcacccaccc ttctcctgct gccctggccg attatctggc cggcgtcgcc acggtggaga
    17341 aaacacaacg acctcgccct gttcgccgtc gtcagcggcg gatatagcgc gaagcaaact
    17401 gattttcccc ggaacgccat cgcgaacgca tggcgttcca ttgactttgt gaatcttagg
    17461 aaacgggacc gattatggat aacttgcgct tctcttctgc gccgacagca gattccattg
    17521 atgcatcgat cgctcaacac tacccggact gcgaacctgt cgcggttatc ggctacgcct
    17581 gccattttcc tgaatcgccg gatggcgaaa cgttctggca aaatctgctg gaaggtcgtg
    17641 aatgcagccg acgctttacg cgcgaagagc ttctggccgt cggtctggat gccgccatca
    17701 ttgacgatcc tcattatgtc aatatcggta cggtgttaga caacgccgac tgcttcgacg
    17761 ccaccctgtt tggctattcg cgacaggaag cggagtcgat ggacccgcag cagcgcctgt
    17821 ttttgcaggc ggtctggcat gcgctggaac atgccggtta tgcccccggc gccgtccccc
    17881 ataagaccgg cgttttcgcc tcttcccgga tgagtaccta ccccggtcgc gaagcattga
    17941 acgtgacaga agtcgcgcag gtaaaaggtc tgcaatctct gatgggcaat gataaagact
    18001 atattgccac ccgcgccgcg tacaaactca acctgcacgg cccggcgtta tcggtacaga
    18061 ccgcctgctc cagctcgctg gttgccgtgc atctggcctg tgaaagcctg cgcgctggcg
    18121 aatccgatat ggcggttgcc ggcggcgtgg cgctctcttt cccccagcag gcaggctacc
    18181 gctaccagcc cggaatgatt ttctctcctg atggtcactg tcgtcccttt gacgcctcgg
    18241 ctgagggcac ctgggccggt aacggtctcg gctgcgtggt gctgcgtcgc ctgagagacg
    18301 cgctgctgtc aggcgatccg attatctcgg tgatcctctc cagcgcggtc aacaacgacg
    18361 gcaacagaaa ggtcggctat accgcccctt ccgtcgcagg gcaacaggca gtcatcgaag
    18421 aggcgttaat gctggcggcc atcgacgaca ggcaggtagg ttacattgaa acccacggca
    18481 ccggcacacc gctgggcgac gcgattgaaa ttgaagcgtt acgcaacgtc tatgcgcctc
    18541 gcccgcagga tcagcgctgt gcgctcggtt ccgtgaaaag taacatgggc catctggata
    18601 ccgcggcggg cattgccgga ctgctgaaaa ccgttctggc agtcagtcgc gggcaaattc
    18661 ctcccttact gaattttcac acccccaacc cggcgctgaa acttgaagag agccccttta
    18721 ccataccggt gtcggcacag gcatggcagg acgaaatgcg ctatgcgggc gtctcctcct
    18781 ttggtattgg cggcaccaac tgccatatga tcgtcgcctc gctgcccgac gcgctcaacg
    18841 cgcgcctccc caatacggat agcggcagaa aaagtaccgc gctgctgctc agcgccgcca
    18901 gcgacagcgc gttgcggcgg ctggcgacgg attatgccgg ggcgctgaga gagaatgcgg
    18961 atgccagctc tctggccttc acagccctgc acgcgcgccg tctcgatctc cccttccgcc
    19021 tggcggcgcc attaaaccgt gaaaccgccg aggcgctcag cgcctgggct agtgagaaat
    19081 cgggggcgct ggtttacagc ggccacggcg ccagcggcaa gcaggtgtgg ctgtttaccg
    19141 gccagggctc gcactggcgc actatgggtc aaacgatgta ccagcactca acggcgtttg
    19201 ccgacacgct ggatcgctgt ttttccgcct gtagcgaaat gctcacgccg tcactgcgcg
    19261 aagcgatgtt taaccccgat tcggcgcagc tggacaatat ggcctgggcg cagccggcga
    19321 ttgtcgcgtt tgaaatcgcg atggcggcgc actggcgtgc tgaaggactg aagccagact
    19381 tcgccattgg gcattccgtc ggtgaatttg ccgctgccgt tgtctgcgga cactatacga
    19441 ttgaacaggt catgccactg gtttgtcggc gcggcgcgct aatgcagcag tgcgcaagcg
    19501 gcgcgatggt ggcggtattt gcagacgaag acacgctgat gccgctggct cgccagtttg
    19561 agctggatct cgccgccaac aacggtacgc aacatacggt attttccggg ccggaagccc
    19621 gtctcgcggt attttgcgcc acgctctcgc agcatgacat taactatcgt cgcctgagcg
    19681 taaccggtgc ggcgcactcc gctttactgg agccgatact cgatcggttc caggacgcct
    19741 gcgcgggact gcacgcggag ccggggcaaa taccgattat ttccacgctc accgccgacg
    19801 tcattgatga gtcaacgctc aaccaggcgg attactggcg ccgacacatg cgccagccgg
    19861 tgcgttttat ccagagtatt caggtggcgc atcagctcgg cacccgcgtt tttctggaga
    19921 tggggcccga tgcccagttg gttgcttgcg ggcagcgcga ataccgcgat aacgcatact
    19981 ggatagccag cgcccggcgt aacaaagagg cgagcgatgt cctcaatcag gccctgctcc
    20041 agctttacgc tgccggcgtc gccctaccgt gggccgacct gctggcgggc gatggacaac
    20101 gtatcgctgc gccatgttat ccgtttgata ctgaacgtta ctggaaagag cgcgtctccc
    20161 cggcctgcga gcctgccgac gcagcgctgt ctgccgggct ggaggtggcg agtcgcgccg
    20221 cgacagcgct cgatctccct cgcctggaag cgcttaaaca gtgcgccacg cgactgcacg
    20281 ccatctacgt cgatcaactg gtacaacgct gtaccggcga tgccatagag aacggcgtgg
    20341 acgccatgac catcatgcgc cgtggacgtc tgctgccccg ctaccagcag ctactccagc
    20401 gcctgctgaa taactgcgtg gtcgacggcg attaccgctg caccgacggg cgatacgtcc
    20461 gcgcccgccc cattgaacat caacagcggg aatcactgct gacggaactt gccggttatt
    20521 gtgaaggttt tcaggctatt cccgacacca tcgcccgtgc cggcgatcgg ttatatgaaa
    20581 tgatgagcgg cgcggaagaa ccggtggcga ttatcttccc gcaaagcgcc tccgacggcg
    20641 tggaagtgct gtatcaggaa ttcagctttg gccgctattt caaccaaatc gccgccgggg
    20701 tattacgcgg cattgtccag acgcgtcagc cccgccagcc gttgcgtatt cttgaagttg
    20761 gcggcggaac cggcggcacc accgcgtggc tgctgccgga actcaacggc gttccggcac
    20821 tggagtacca tttcaccgat atctcggcgc tgttcacccg tcgcgcccag cagaaattcg
    20881 ccgactatga ttttgtgaag tatagcgagc tggatctcga aaaagaggcg cagtctcagg
    20941 gtttccaggc acagtcttac gatcttatcg tggcagcgaa cgtgattcac gccacccgcc
    21001 atattggccg cacgctcgat aatctgcgcc ccctgctcaa gccgggcggg cgcctgctga
    21061 tgcgcgaaat cacccagcca atgcgtctgt ttgacttcgt tttcggcccg ctggttcttc
    21121 cgctacagga tctcgacgcc cgcgaaggtg agttattcct caccaccgct cagtggcaac
    21181 aacagtgccg ccacgccgga ttcagcaaag tggcgtggct accgcaggat ggcagcccga
    21241 ccgccgggat gagcgaacat atcattctcg ccacgctgcc cggtcaggcg gttagcgccg
    21301 taacattcac cgcgccatca gaacccgtgt tggggcaggc gctgacggat aacggtgatt
    21361 atctcgccga ctggtctgat tgcgcaggtc agcccgaaca gtttaacgcc cgctggcagg
    21421 aggcctggcg tctgctttca cagcgtcatg gcgacgctct gcctgtggaa ccgccccccg
    21481 tcgccgcccc ggagtggctg gggaaggttc gcttaagctg gcaaaacaaa gccttttccc
    21541 gcggtcagat gcgcgttgaa gcccgtcatc ctgctggcga gtggctgccg ctatcgcccg
    21601 ccgcgcctct tcctgcgccg caaacgcatt atcaatggcg ctggacgccc ctcaacgtcg
    21661 ccagcattga ccatccgctt acctttagct tcagcgccgg tacgcttgcg cgcagcgacg
    21721 agctggcgca atacggcatc attcacgatc cgcacgcctc ttcacgactg atgattgttg
    21781 aggagagcga ggatacgctg gccttagcgg agaaagtgat agcagcgctc accgccagcg
    21841 cagccggatt gattgtggtt actcgccgcg cgtggcgagt cgaggaaaat gaagcactct
    21901 ctgcatccca tcacgcgcta tgggccttgc ttcgcgtcgc ggccaacgaa cagccggaac
    21961 ggttgcttgc cgccatcgat ctcgccgaaa acacctcgtg ggaaacgctg catcaagggt
    22021 tgagcgcagt ctcactatca cagcgctggc tcgccgcacg gggtgacacc ctttggctcc
    22081 cttcactgtc gcccaatacg ggatgcgccg ctgaattacc ggcaaacgtg tttaccggcg
    22141 atagccgctg gcatctggtg accggagcgt ttggcggatt aggccgcctt gccgtgaact
    22201 ggctcagaga aaaaggggcg cgacgcatcg ccctgctggc gccgcgcgtg gatgagtcat
    22261 ggctacgcga cgtggagggc gggcagacgc gcgtctgccg ttgtgatgtg ggcgatgccg
    22321 ggcaactggc cacggttctt gacgatctgg cggccaacgg cggcattgcc ggagcgattc
    22381 atgccgctgg cgtattggct gacgcgccct tgcaggagct tgatgaccac cagctggctg
    22441 ccgttttcgc ggtaaaagcg caggcggcaa gccagctgtt gcaaaccctg cgcaaccacg
    22501 acggacgcta tcttattctc tactcttccg ctgccgccac cctcggcgcg ccgggtcaga
    22561 gcgcccatgc gctggcctgc ggctacctgg acgggctggc ccagcagttt tccacccttg
    22621 atgcgccgaa aacgctctct gtcgcctggg gcgcatgggg agaaagcggt cgggcggcca
    22681 cgccggaaat gctggcgacg ctcgccagcc gaggtatggg cgcgttaagc gatgccgaag
    22741 gctgctggca cctggaacag gcggtgatgc gcggcgcccc gtggcgactg gcgatgcgcg
    22801 tttttaccga caaaatgccc ccgttacaac aggctctgtt taacatcagc gccacagaaa
    22861 aagccgcaac gccggtcatt cctcctgctg atgacaacgc ctttaacggc agcctgagcg
    22921 atgaaacagc ggtgatggca tggctgaaaa agcggattgc ggttcagcta aggctgagcg
    22981 atccggcgtc actgcatcca aaccaggatc tgttgcaact cggcatggac tcgctgctct
    23041 tccttgaact cagtagcgat attcagcact acctgggcgt acgcatcaat gcggaacggg
    23101 cgtggcagga tctgtctcct catggactca cgcagcttat ctgttctaag ccagaggcga
    23161 cgcctgccgc ttcgcagccg gaagtgttgc ggcacgacgc cgacgagcgt tatgcgccct
    23221 tccctttgac gcccattcag cacgcctact ggctggggcg aacccacctc attggctatg
    23281 gcggcgtcgc ctgtcacgtc ctgtttgagt gggataaacg ccacgatgag ttcgatctcg
    23341 ccatactgga gaaagcatgg aaccagctca tcgcacgcca cgatatgttg cgtatggtgg
    23401 ttgatgccga cgggcagcag cgaatcctgg cgacaacgcc ggagtatcac atcccgcgtg
    23461 acgatctgcg cgcgctttcc ccggaagaac agcgcatcgc gctggaaaaa cggcggcatg
    23521 aactgagcta tcgcgttttg cctgccgacc agtggcctct ttttgagctg gtggtcagcg
    23581 aaatcgacga ttgccattac cgtctgcata tgaacctcga ccttttgcag tttgatgtgc
    23641 agagttttaa agtcatgatg gacgacctgg cgcaggtctg gcgcggtgaa acgctggcgc
    23701 cgctcgctat taccttccgt gattatgtga tggctgaaca ggcgcgccga cagacatcgg
    23761 catggcacga tgcctgggat tactggcagg aaaaactgcc gcaactgccc ttagcgccag
    23821 agctgccggt ggttgagacg cccccggaaa cgccacactt caccaccttc aaatcgacga
    23881 tcggcaagac agaatggcag gccgtgaaac agcgctggca gcagcaaggc gtcacaccgt
    23941 ctgccgcgct gctcacgctg tttgccgcta cccttgagcg ctggagccgt accacaacat
    24001 ttacgctgaa cctgacgttc ttcaatcgcc agccgatcca tccgcaaatc aaccagttga
    24061 ttggtgattt tacctccgtc acgctggttg attttaactt ctcagcgccg gtgacgttgc
    24121 aagagcagat gcaacagacc caacagcgcc tctggcaaaa catggcgcac agtgaaatga
    24181 acggtgttga ggtgatccgt gagctgggcc gcctgcgcgg atcacaacgt caaccgctga
    24241 tgccggtagt gtttaccagt atgctgggga tgacgctgga aggcatgact atcgatcagg
    24301 cgatgagcca tctgttcggc gaaccctgct atgtattcac gcaaacgccg caggtctggc
    24361 tggatcatca ggtcatggag agcaacggcg agttgatgtt tagctggtac tgcatggaca
    24421 acgtgctgga acccggcgct gccgaggcga tgtttaatga ctattgcgct atcctgcaag
    24481 ccgtcatcgc cgcccctgaa agcctgaaga ctctcgccag cggcatcgcc aggcacattc
    24541 cccgccgacg ctggccgctg aacgcgcagg cggactacga cctgcgggat attgagcagg
    24601 cgacgctcga ataccccggc atccggcagg ccagagcgga aataaccgaa cagggcgcgt
    24661 tgacgctgga tatcgtgatg gccgacgatc cttcgccatc agcggcgatg cctgatgagc
    24721 acgaacttac ccaactggcg ctgccgttgc ctgagcaggc gcagcttgat gagctggagg
    24781 cgacctggcg ctggctggag gcgcgtgcgc tacaggggat cgcggctacg ctaaatcgtc
    24841 acggcctgtt taccacgccg gagatcgccc atcgctttag cgcaatagta caggcgctgt
    24901 ccgcgcaagc gtctcaccag cgtctgctgc gccagtggct acagtgtctg acggaaagag
    24961 agtggttaat ccgcgaaggt gaaagctggc gctgccgcat tccgctcagc gagattcctg
    25021 agcctcagga agcgtgcccg caaagccaat ggagccaggc gctggcgcag tatctggaaa
    25081 cctgcatcgc ccggcacgac gccctcttct ccgggcagtg ttctccgctg gaattgctgt
    25141 tcaacgagca gcatcgcgtt accgacgcgc tgtatcgcga caaccccgcc agcgcctgtc
    25201 tgaatcgcta taccgcgcag attgccgcct tgtgcagcgc agaacggatt ctggaggttg
    25261 gcgccggaac cgcagccact accgcgccgg tgctgaaggc cacgcggaac acgcgacagt
    25321 cgtaccactt cacggacgtc tccgcgcagt tcctcaatga cgccagagcc cgtttccatg
    25381 atgaatcgca ggtgtcttat gccttgttcg acatcaacca gccgctggat ttcaccgccc
    25441 acccggaggc gggttacgac ctgatcgttg ccgtcaatgt gctccacgac gccagccatg
    25501 tcgtccagac gttgcgcaga ttaaaactgt tgctgaaagc cggcggacgt ttgctgatcg
    25561 ttgaagcgac ggagcgaaac agcgtattcc agctggcgag cgtgggcttt attgagggat
    25621 taagcggata ccgcgatttc cgccgccggg atgagaaacc gatgctcacc cgctccgcat
    25681 ggcaggaggt tctcgttcag gccgggtttg caaacgagct ggcgtggccc gcgcaggaat
    25741 cgtcgccgct gcgccagcat ctgctggtag cgcgttcgcc tggcgtaaat cgcccggata
    25801 aaaaagccgt gagccgctat ttacagcagc gctttggcac cggtctgccc attttacaga
    25861 tccggcaaag agaagcgtta tttacgccgc tgcatgcccc gtctgatgcg ccgactgagc
    25921 cagccaaacc cacgccagtt gccgggggga atccggcgct ggaaaaacag gtggctgaac
    25981 tctggcaatc gctgctgtct cgccccgtgg caaggcatca cgactttttc gaactgggcg
    26041 gcgacagcct gatggcgaca aggatggtcg cgcagctaaa ccggagaggg attgccaggg
    26101 ctaaccttca ggatctgttc agccattcga cgctgagcga cttctgcgcc catctacagg
    26161 cggctacgtc aggagaggac aacccgatcc ccctttgcca gggcgacggt gaggaaaccc
    26221 tgtttgtctt ccacgcttca gacggcgata tcagcgcctg gctgccgctc gctagcgcgt
    26281 tgaacaggcg cgttttcggc ctgcaagcaa aatcgccgca gcgctttgcc acgctcgacc
    26341 agatgatcga tgagtatgtc gggtgcatcc gtcgtcagca gcctcacggc ccttatgtgc
    26401 tggcgggttg gtcgtatggc gcgtttcttg cggcgggcgc cgcacaacgc ctgtacgcca
    26461 aaggcgagca ggttcggatg gtgttaatcg atcccgtgtg ccgacaggat ttctgttgcg
    26521 aaaaccgggc ggccctgctg cgcctgttag ccgaaggaca aacgcctctg gcactgcccg
    26581 aacatttcga ccagcagacg cccgacagcc agcttgccga ctttatcagc ctcgctaaaa
    26641 cggccggtat ggtgtcgcaa aacctgacgc tgcaagcggc agaaacgtgg ctcgccaaca
    26701 tcgcgcatct gctgcgttta ctgactgagc atacgccggg cgaaagcgtt ccggtcccct
    26761 gtctcatggt gtatgccgcc gggagaccct cgcgctggac gccagcagaa accgagtggc
    26821 agggctggat aaacaacgcc gacgacgctg tgattgaagc cagccactgg caaatcatga
    26881 tggaagcccc ccacgttcag gcttgtgcgc aacacattac gcgctggctt tgcgcaacct
    26941 caacgcaacc ggagaacacg ttatgatgcc gtccgcctcc ccaaaacaac gcgtactgat
    27001 tgtgggcgcc aaatttggcg aaatgtacct gaatgccttt atgcagcccc cggaggggct
    27061 ggaactggtc ggcctgctgg cgcagggaag cgcccgttca agagagctgg ctcatgcgtt
    27121 tggcattccg ctgtatacct cgccggaaca gataaccagg atgccggata tcgcctgtat
    27181 tgtcgtgcgt tcgaccgtcg ccggaggaac cggtacgcag cttgccagac actttctgac
    27241 gcgcggcgta cacgtcattc aggagcatcc cctccacccg gatgacatca gttccttaca
    27301 aacgctggcg caggaacagg gctgctgcta ttgggtaaac acgttttatc cgcatacgcg
    27361 cgcgggacgc acatggcttc gcgatgcgca gcagctgcgt cgctgcctgg ctaagacgcc
    27421 gccggtagtc cacgccacca ccagccgaca gttgctctac tccactctgg atttgttgtt
    27481 gctggctctg ggcgttgatg ccgccgccgt ggagtgcgac gtggttggca gctttagcga
    27541 ttttcactgt ctgagactct tctggccgga gggagaagcc tgcctgctgc ttcagcgcta
    27601 tctcgatcct gacgatccag atatgcatag ccttatcatg caccgcctgc tgctgggctg
    27661 gccggaaggt catctttcgc tggaggcgag ctacggcccc gtcatctggt catccaacct
    27721 gtttgtcgca gaccatcagg agaacgccca cagcctctac cgcagaccgg agatcctgcg
    27781 cgatctgccg ggtctgacgc gcagcgcggc gcccctgagc tggcgcgact gctgcgaaac
    27841 cgtcgggccg gagggcgtca gttggttgct acaccagttg cgcagccatc tggcggacga
    27901 acatccacca gcggcctgcc aaagcgtaca tcagatcgcg ttatcacgtt tatggcagca
    27961 gattttacgt aagaccggca atgcggagat cagacgcctg acgccgccgc accacgaccg
    28021 gttagccggt ttctacaacg atgatgataa ggaggcgctg tgacgcaatc tgcaatgtgc
    28081 atcccgctgt ggcccgcccg gaacggcaat actgcgcatc tggtcatgtg ccctttcgct
    28141 ggcggcagca gtagcgcgtt tcgccactgg caagctgagc aactggctga ttgcgcgctt
    28201 tctctggtga cctggccggg gcgcgatcgc cttcgccatc tggaaccgct cagaagcatt
    28261 acacaactgg cggcactgct ggcgaacgag ctggaagcat ccgtatcgcc tgacacgccg
    28321 cttttactcg ccgggcacag catgggggcg caggtggcgt ttgaaacctg ccgacttctg
    28381 gagcaacggg ggcttgcgcc acaaggactg attatttccg ggtgccatgc cccgcatctg
    28441 cattctgaac gccagctcag ccatcgcgat gatgccgact ttatcgctga gctgatagac
    28501 attggcggat gttctcctga actgcgggaa aaccaggaat taatgtcgct gtttcttcct
    28561 cttctgcgcg ctgattttta cgccaccgag agctatcact acgactcgcc cgacgtctgt
    28621 ccgccgctgc gcacgcctgc gctgttattg tgcggcagcc acgatcgcga agcctcctgg
    28681 cagcaggtcg atgcctggcg tcagtggctg agccacgtta caggcccggt ggtgattgac
    28741 ggcgatcatt tctatcccat tcaacaagcc cggtcctttt ttacgcagat tgtccgccat
    28801 tttccccacg cattttctgc aatgaccgcg ttgcaaaaac agcccagtac ttcagaaagg
    28861 tgacgcatga attcttcctt tgaatctctg attgaacagt atcccttacc cattgccgaa
    28921 cagttgcgcc actgggcggc ccgttatgcc tcgcgaattg ccgtcgttga tgcaaagggg
    28981 tcgttaacct acagcgcgct tgatgcacaa gttgacgaac ttgccgcagg tctgtcatca
    29041 ctgggtttgc gttcggggga gcatgtaatt gtgcagcttc ccaacgacaa cgcgtttgtt
    29101 accctgctgt tcgccttgtt aagactgggc gttatccccg tgctggcgat gccctcgcaa
    29161 cgggctctgg atatcgacgc gctgattgag ctggcgcaac ccgtcgctta cgttattcac
    29221 ggggaaaacc acgcagagct ggcccgacag atggcgcaca aacacgcctg cttgcgtcat
    29281 gttctggtcg ctggagagac cgtgagcgac gattttacgc cgctcttctc ccttcacggt
    29341 gagcgacagg catggccgca gcctgatgtt tccgccaccg cgttgttgtt gctctcaggc
    29401 ggcacaaccg gcacgcccaa actcatcccg cgccgacatg ccgactatag ctataacttc
    29461 agcgcttctg ctgaactgtg cggcatcagc caacagagcg tgtatctcgc cgtcctcccg
    29521 gtggcgcata actttccgct ggcctgcccc ggtattctgg gaacgcttgc ctgcggcgga
    29581 aaagtggtgc tgaccgacag cgccagctgt gatgaggtga tgcctttaat cgcgcaggaa
    29641 agagtgactc acgtcgccct ggttccggcg ctggcgcaat tatgggtgca ggccagggag
    29701 tgggaagaca gcgacctttc gtcgctgcgc gtcattcagg caggcggcgc ccggctcgac
    29761 ccgacgcttg ctgagcaggt tatcgccacc tttgactgta ccctgcaaca ggttttcggt
    29821 atggcggaag gcctgctctg ttttacccgg ctggacgatc cgcatgccac cattctccac
    29881 agccaggggc gcccgttgtc ccctctggat gaaatccgca tcgttgatca agacgagaac
    29941 gacgtcgcgc cgggcgaaac cgggcaattg ttaacgcgcg gcccttatac catttcgggc
    30001 tattaccgcg cccctgccca caacgcgcag gcctttaccg cgcaagggtt ttaccgcaca
    30061 ggcgacaatg tcaggctgga tgaggtgggg aacctgcacg ttgagggacg cataaaagag
    30121 cagatcaacc gcgccggaga aaaaatagcc gcggctgaag tggaatcggc actgctgcgt
    30181 ttagcggaag tgcaagattg cgcggtggtc gccgcgccgg acacgctgct tggcgagcgg
    30241 atttgcgcgt ttatcatcgc gcagcaggtg ccaactgact atcagcagtt gcgtcaacaa
    30301 ctgacccgta tggggctcag cgcgtggaaa attcctgacc aaatcgagtt tctggaccac
    30361 tggccgctca ccgccgtcgg caagatagac aaaaaacgcc tgacggctct cgccgtcgac
    30421 cgttatcgcc attctgccca ataagcgcaa accgacccga aacaggttga aataaacccg
    30481 tttcgggtag cactactatt agaaatagtt atcattttca attcaccatt gtcggtattt
    30541 ttggcgtttc gccgtcttac agggactcac aacaatgaaa atgacacggc tttatcctct
    30601 ggccttgggg ggattattgc tccccgccat tgctaatgcc cagacttcac agcaagacga
    30661 aagcacgctg gtggttaccg ccagtaaaca atcttcccgc tcggcatcag ccaacaacgt
    30721 ctcgtctact gttgtcagcg cgccggaatt aagcgacgcc ggcgtcaccg ccagcgacaa
    30781 actccccaga gtcttgcccg ggctcaatat tgaaaatagc ggcaacatgc ttttttcgac
    30841 gatctcgcta cgcggcgtct cttcagcgca ggacttctat aaccccgccg tcaccctgta
    30901 tgtcgatggc gtccctcagc tttccaccaa caccatccag gcgcttaccg atgtgcaaag
    30961 cgtggagttg ctgcgaggcc cacagggaac gttatatggc aaaagcgctc agggcgggat
    31021 catcaacatc gtcacccagc agccggacag cacgccgcgc ggctatattg aaggcggcgt
    31081 cagtagccgc gacagttatc gaagtaagtt caacctgagc ggccccattc aggatggcct
    31141 gctgtacggc agcgtcaccc tgttacgcca ggttgatgac ggcgacatga ttaaccccgc
    31201 gacgggaagc gatgacttag gcggcacccg cgccagcata gggaatgtga aactgcgtct
    31261 ggcgccggac gatcagccct gggaaatggg ctttgccgcc tcacgcgaat gtacccgcgc
    31321 cacccaggac gcctatgtgg gatggaatga tattaagggc cgtaagctgt cgatcagcga
    31381 tggttcacca gacccgtaca tgcggcgctg cactgacagc cagaccctga gtgggaaata
    31441 caccaccgat gactgggttt tcaacctgat cagcgcctgg cagcagcagc attattcgcg
    31501 caccttccct tccggttcgt taatcgtcaa tatgcctcag cgctggaatc aggatgtgca
    31561 ggagctgcgc gccgcaaccc tgggcgatgc gcgtaccgtt gatatggtgt ttgggctgta
    31621 ccggcagaac acccgcgaga agttaaattc agcctacgac atgccgacaa tgccttattt
    31681 aagcagtacc ggctatacca ccgctgaaac gctggccgca tacagtgacc tgacctggca
    31741 tttaaccgat cgttttgata tcggcggcgg cgtgcgcttc tcgcatgata aatccagtac
    31801 acaatatcac ggcagcatgc tcggcaaccc gtttggcgac cagggtaaga gcaatgacga
    31861 tcaggtgctc gggcagctat ccgcaggcta tatgctgacc gatgactgga gagtgtatac
    31921 ccgtgtagcc cagggatata aaccttccgg gtacaacatc gtgcctactg cgggtcttga
    31981 tgccaaaccg ttcgtcgccg agaaatccat caactatgaa cttggcaccc gctacgaaac
    32041 cgctgacgtc acgctgcaag ctgcgacgtt ttatacccac accaaagaca tgcagcttta
    32101 ctctggcccg gtcgggatgc agacattaag caatgcgggt aaagccgacg ccaccggcgt
    32161 tgagcttgaa gcgaagtggc ggtttgcgcc aggctggtca tgggatatca atggcaacgt
    32221 gatccgttcc gaattcacca atgacagtga gttgtatcac ggtaaccggg tgccgttcgt
    32281 accacgttat ggcgcgggaa gcagcgtgaa cggcgtgatt gatacgcgct atggcgcact
    32341 gatgccccga ctggcggtta atctggtcgg gccgcattat ttcgatggcg acaaccagtt
    32401 gcggcaaggc acctatgcca ccctggacag cagcctgggc tggcaggcga ctgaacggat
    32461 gaacatttcc gtctatgtcg ataacctgtt cgaccgtcgt taccgtacct atggctacat
    32521 gaacggcagc agcgccgtcg cgcaggtcaa tatgggtcgc accgtcggta tcaatacgcg
    32581 aattgatttc ttctgattat tgtaaaaggg ataccgaaaa ggtatccctt ttacaccact
    32641 agttaaaacc agtaactcag cagagtcgca aaaaatatta atccatagtg attatttaaa
    32701 caatgaaatt gcgattagga taaatagatt taactttctc gttcctttct ctccttatac
    32761 taaagaaata atcatatcaa aataaaaatt cacaacagtg caacat
//
LOCUS       DAPHJE010000043        32308 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPHJE010000043 DAPHJE010000000
VERSION     DAPHJE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.043"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(68..1828)
                     /locus_tag="RH222_002065"
     CDS             complement(68..1828)
                     /locus_tag="RH222_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDV3797083.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1958..2650
                     /locus_tag="RH222_002066"
     CDS             1958..2650
                     /locus_tag="RH222_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV3797084.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2849..3937
                     /gene="serC"
                     /locus_tag="RH222_002067"
     CDS             2849..3937
                     /gene="serC"
                     /locus_tag="RH222_002067"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV3797085.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4008..5291
                     /gene="aroA"
                     /locus_tag="RH222_002068"
     CDS             4008..5291
                     /gene="aroA"
                     /locus_tag="RH222_002068"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV3797086.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5460..6224
                     /gene="ycaL"
                     /locus_tag="RH222_002069"
     CDS             5460..6224
                     /gene="ycaL"
                     /locus_tag="RH222_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV3797087.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6397..7080
                     /gene="cmk"
                     /locus_tag="RH222_002070"
     CDS             6397..7080
                     /gene="cmk"
                     /locus_tag="RH222_002070"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV3797088.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7191..8864
                     /gene="rpsA"
                     /locus_tag="RH222_002071"
     CDS             7191..8864
                     /gene="rpsA"
                     /locus_tag="RH222_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV3797089.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9024..9308
                     /gene="ihfB"
                     /locus_tag="RH222_002072"
     CDS             9024..9308
                     /gene="ihfB"
                     /locus_tag="RH222_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV3797090.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9515..11779
                     /locus_tag="RH222_002073"
     CDS             9515..11779
                     /locus_tag="RH222_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV3797091.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPSLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11816..13564
                     /gene="msbA"
                     /locus_tag="RH222_002074"
     CDS             11816..13564
                     /gene="msbA"
                     /locus_tag="RH222_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV3797092.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13561..14547
                     /gene="lpxK"
                     /locus_tag="RH222_002075"
     CDS             13561..14547
                     /gene="lpxK"
                     /locus_tag="RH222_002075"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV3797093.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14584..15816
                     /locus_tag="RH222_002076"
     CDS             14584..15816
                     /locus_tag="RH222_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV3797094.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15868..16050
                     /gene="ycaR"
                     /locus_tag="RH222_002077"
     CDS             15868..16050
                     /gene="ycaR"
                     /locus_tag="RH222_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV3797095.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16047..16793
                     /gene="kdsB"
                     /locus_tag="RH222_002078"
     CDS             16047..16793
                     /gene="kdsB"
                     /locus_tag="RH222_002078"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV3797096.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16947..17840
                     /locus_tag="RH222_002079"
     CDS             16947..17840
                     /locus_tag="RH222_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV3797097.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17817..18596)
                     /gene="elyC"
                     /locus_tag="RH222_002080"
     CDS             complement(17817..18596)
                     /gene="elyC"
                     /locus_tag="RH222_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV3797098.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18732..19517
                     /gene="cmoM"
                     /locus_tag="RH222_002081"
     CDS             18732..19517
                     /gene="cmoM"
                     /locus_tag="RH222_002081"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV3797099.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19514..20836
                     /gene="mukF"
                     /locus_tag="RH222_002082"
     CDS             19514..20836
                     /gene="mukF"
                     /locus_tag="RH222_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV3797100.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20817..21521
                     /gene="mukE"
                     /locus_tag="RH222_002083"
     CDS             20817..21521
                     /gene="mukE"
                     /locus_tag="RH222_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV3797101.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21521..25981
                     /gene="mukB"
                     /locus_tag="RH222_002084"
     CDS             21521..25981
                     /gene="mukB"
                     /locus_tag="RH222_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV3797102.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTSDSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26242..28089
                     /gene="ldtD"
                     /locus_tag="RH222_002085"
     CDS             26242..28089
                     /gene="ldtD"
                     /locus_tag="RH222_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3797103.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28270..28818
                     /locus_tag="RH222_002086"
     CDS             28270..28818
                     /locus_tag="RH222_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV3797104.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28845..29492
                     /gene="gloC"
                     /locus_tag="RH222_002087"
     CDS             28845..29492
                     /gene="gloC"
                     /locus_tag="RH222_002087"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV3797105.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29714..30904)
                     /gene="aspC"
                     /locus_tag="RH222_002088"
     CDS             complement(29714..30904)
                     /gene="aspC"
                     /locus_tag="RH222_002088"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV3797106.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSASLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31089..32177)
                     /gene="ompF"
                     /locus_tag="RH222_002089"
     CDS             complement(31089..32177)
                     /gene="ompF"
                     /locus_tag="RH222_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV3797107.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7844 a         7859 c         8705 g         7900 t
ORIGIN      
        1 ttaaaaaaac gccccgtaat ataactcaga ctaatcatta agcctacatt gcgtaggcta
       61 tttgatttta ttttgcccag aatgctgctt tggcgcgctg cagcttttcg taggccttca
      121 acaacgactg atgtgcagca aacgcgtgca gatcgctatc taccggttgc aggccgtaaa
      181 acgccgcttc gccgctcatt gccgcactgg cggcttctac ggcatctgcg ccgtacatgc
      241 gaacaaacgc attcagatat tgcagcggct ggcgatcttc ttcctgtgcc agtaataaca
      301 acgtttgcag gcagcgataa tagttggcgc gttccggact aaatactgat gagttaaact
      361 ccatcgtcca ttcggtccag accagagcct gttccagatc gccaccagcc agcgccagca
      421 tggcttttaa ttcaccgata cgcagggtgt accaaccgtt atccgacccg gtcgccagac
      481 ccaacagctc acgcacgcgg gtaaagtcat caaaaccttc ttcatccagt tgctcgatga
      541 ggttcaggta atcttctttt tcccactcgc tgcctggtag cgaaagaatc gtttcacgta
      601 aatggctgcc catactgtta ttcgcgagcc acagatcttc agccggataa atatcggaca
      661 tgccaggcac gataatgcgg caagcgtaga cacccagatg ctcgtaatcg gcaatataaa
      721 cttctttatc ttcctttttg aagatagcca tcagcgtggc gaactcttct tccgtggtgc
      781 cggagaaatt ccagtccaca aacggataat cggcatcctg cttgaacagg tcccaggaga
      841 ttaaaccgct ggaatcgata aagtgcgttt cgaggttggt atgttcagcg acttcttcat
      901 catcgaaggt tggcggagta aacacatcca aatctttcag gccacgacct tgcagcagct
      961 cggtcacggt acgttccagt gctacgccaa aatcaggatg cgcaccgaaa gaggcaaagc
     1021 aggtaccgtt agcaggattg aacagtacca cgcaaatcac cggatactgg ccgccaagcg
     1081 aaccgtcata agcgaagatt gggaaaccct ccgcttccag cgtttcgatg gcttcaacta
     1141 ctgctgggta acgcgccagc acgtctgccg ggatctctgg caggctgatg ctttcagcaa
     1201 taatgcggtt tttcacgtag cgttcgaaaa cttcagacaa tccctgaacg cgtgcttcgt
     1261 tgcgggtatt acctgcggac ataccgttgg agacgtacag gttaccaatg atattcatcg
     1321 gaatataaac ggtctgattg tcggactgac gcgtaaacgg caggccgcaa ataccacgat
     1381 cttcgttacc ggattgtagg tcaatcagca tgctgccggt cagttcattc tccggatcat
     1441 aaaacgcgcg cagacggtca tcgagtagtc cttctggcac atcgtcattt tcggtcagtg
     1501 ggaaccattt ttcgttggga taatgcacga acggaccgtt ggcgatggtt tcgcccagcc
     1561 agaaatcagc aaaaaagtag ttggttgaga gacgctcgaa atattcacca agtgcagaag
     1621 ccagcgccgc ttttttggtt gcgcctttac cgttggtaaa acacagtgcg cactctttgt
     1681 cgcgaatatg tacagaccag acgttaggca caggattcag ccaggaggcc tcttcaatct
     1741 gaaagccgag gtctgaaagt ttttgctgga agcgagcgat ggaatcttcc agagcggcat
     1801 ctttgccggg gataaatgtt tgcgtcatga aaatcacttt agtcgtacgg aaagcgcgca
     1861 ataatacggg ttttatctca aaggcgctat caccgccgcc atgccgatga actgttgact
     1921 atgcttttag cggataaacc acgataagag cataaaaatg aaggcgttcg atctccaccg
     1981 tatggcattt gataaagtgc cttttgattt ccttggcgaa gttgcactac gtagtcttta
     2041 tacctttgta ctggtttttt tgttcctcaa aatgaccgga agacgcggtg tgcgacagat
     2101 gtcgctgttt gaagtgttaa tcattctcac gctgggttca gcggcgggag atgtggcgtt
     2161 ttatgatgat gtgccgatgg tgccggtact tatcgtcttt atcactctgg cgttgttata
     2221 ccgcctggta atgtggttga tggcgcacag tgaaaaactg gaagatttac tggaaggtaa
     2281 gccggttgtt attattgaag atggcgagct ggcctggtcg aaactcaata actccaacat
     2341 gacggaattt gagttcttta tggagctacg attgcgtggc gtggagcagc tggggcaggt
     2401 acgtctggcg attctcgaaa ccaacgggca aatcagtgtc tatttctttg aagatgacaa
     2461 ggtgaaaccg ggtttactta ttttacccag tgattgtacg cagcgttaca aagtggtgcc
     2521 ggagtcggcg gactatgcct gtattcgttg tagtgaaatc attcatatga aagcggggga
     2581 aaaacaatta tgtccgcgct gtgcaaatcc agaatggacg aaggcaagtc gggcaaaacg
     2641 ggtgacctga cagtaaaaac atcggctttt tgctaataat ccgagagatt cttttgtgtg
     2701 atgcaagcca catttttgcc ctcaacggtt ttactcattg cgatgtgtgt cactgaatga
     2761 taaaaccgat agccacagga ataatgtatt acctgtggtc gcaatcgatt gaccgcgggt
     2821 taatagcaac gcaacgtggt gaggggaaat ggctcaaatc ttcaatttta gttctggtcc
     2881 ggcaatgcta ccggcagagg tgcttaaaca ggctcaacag gaactgcgcg actggaacgg
     2941 tcttggtacg tcggtgatgg aagtgagtca ccgtggcaaa gagttcattc aggttgcaga
     3001 ggaagccgag aaggattttc gcgatcttct taatgtcccc tccaactaca aggtattatt
     3061 ctgccatggc ggtggtcgcg gtcagtttgc tgcggtaccg ctgaatattc tcggtgataa
     3121 aaccaccgca gattatgttg atgccggtta ctgggcggca agtgccatta aagaagcgaa
     3181 aaaatactgc acgcctaatg tctttgacgc caaagtgact gttgatggtc tgcgcgcggt
     3241 taagccaatg cgtgaatggc aactctctga taatgctgct tatatgcatt actgcccgaa
     3301 tgaaaccatc gacggtatcg ccatcgacga aacgccagac ttcggcaaag atgtggtggt
     3361 cgccgccgac ttctcttcaa ccattctttc ccgtccgatt gacgtcagcc gttatggcgt
     3421 gatttacgct ggcgcgcaga aaaatatcgg cccggctggc ctgacaatcg tcatcgttcg
     3481 tgaagatttg ctgggcaaag cgaatatcgc gtgtccgtcg attctggatt attccatcct
     3541 caacgataac gactccatgt ttaacacgcc gccgacattt gcctggtatc tatctggtct
     3601 ggtctttaaa tggctgaaag cgaacggcgg tgtagctgaa atggataaaa tcaatcagca
     3661 aaaagcagaa ctgctatatg gggtgattga taacagcgat ttctaccgca atgacgtggc
     3721 gaaagctaac cgttcgcgga tgaacgtgcc gttccagttg gcggacagtg cgcttgacaa
     3781 attgttcctt gaagagtctt ttgctgctgg ccttcatgca ctgaaaggtc accgtgtggt
     3841 cggcggaatg cgcgcttcta tttataacgc catgccgctg gaaggcgtta aagcgctgac
     3901 agacttcatg gttgagttcg aacgccgtca cggttaatgc cgaaattttg cttaatcccc
     3961 acagccagcc tgtggggttt ttatttctgt tgtagagagt tgagttcatg gaatccctga
     4021 cgttacaacc catcgctcgt gtcgatggca ctattaatct gcccggttcc aagagcgttt
     4081 ctaaccgcgc tttattgctg gcggcattag cacacggcaa aacagtatta accaatctgc
     4141 tggatagcga tgacgtgcgc catatgctga atgcattaac agcgttaggg gtaagctata
     4201 cgctttcagc cgatcgtacg cgttgcgaaa ttatcggtaa cggcggtcca ttacacgcag
     4261 aaggtgccct ggagttgttc ctcggtaacg ccggaacggc aatgcgtccg ctggcggcag
     4321 ctctttgtct gggtagcaat gatattgtgc tgaccggtga gccgcgtatg aaagaacgcc
     4381 cgattggtca tctggtggat gcgctgcgcc tgggcggggc gaagatcact tacctggaac
     4441 aagaaaatta tccgccgttg cgtttacagg gcggctttac tggcggcaac gttgacgttg
     4501 atggctccgt ttccagccaa ttcctcaccg cactgttaat gactgcgcct cttgcgccgg
     4561 aagatacggt gattcgtatt aaaggcgatc tggtttctaa accttatatc gacatcacac
     4621 tcaatctgat gaagacgttt ggtgttgaaa ttgaaaatca gcactatcaa caatttgtcg
     4681 taaaaggcgg gcagtcttat cagtctccgg gtacttattt ggtcgaaggc gatgcatctt
     4741 cggcttctta ctttctggca gcagcagcaa tcaaaggcgg cactgtaaaa gtgaccggta
     4801 ttggacgtaa cagtatgcag ggtgatattc gctttgctga tgtgctggaa aaaatgggcg
     4861 cgaccatttg ctggggcgat gattatattt cctgcacgcg tggtgaactg aacgctattg
     4921 atatggatat gaaccatatt cccgatgcgg cgatgaccat tgccacggcg gcgttatttg
     4981 caaaaggcac caccacgctg cgcaatatct ataactggcg tgttaaagaa accgatcgcc
     5041 tgtttgcgat ggcaacagaa ctgcgtaaag tcggtgcgga agtagaagag gggcacgatt
     5101 acattcgtat cactccaccg gaaaaactga actttgccga gatcgcgaca tacaatgatc
     5161 accggatggc gatgtgtttc tcgctggtgg cgttgtcaga tacaccagtg acgattcttg
     5221 atcccaaatg tacggccaaa acatttccgg attatttcga gcagttggcg cggattagcc
     5281 aggcagcctg aatgaacaac gggcaataaa tagccaaatc tttctttatc aaaacgtcgg
     5341 cacattgtcg gcgttttttt tcggaccttg tgagtcattt tgattaatgg tagcgtcgct
     5401 tgtcaatgta atttgttgat acataatatt tatatatgat taatcaacgg atgattcaca
     5461 tgaagaatac taaattactg ctggcgattg cgacctctgc agcattactg acagggtgtc
     5521 aaaataccca cggtattgat accaatatgg ctatcagctc cggtttaaat gcctataaag
     5581 cagcaacatt aagcgatgcc gatgcaaaag cgattgccaa tcagggctgt gccgaaatgg
     5641 acagcggcaa tcaagtcgca agtaaatcca gcaagtacgg taaacgtctg gcaaaaatcg
     5701 ccaaagcatt gggtaacaat attaatggca cgccggtcaa ctataaggtt tatatgacca
     5761 gcgacgtcaa cgcatgggcg atggcgaacg gctgtgtccg tgtctacagt ggcctgatgg
     5821 acatgatgaa tgacaacgaa attgaaggtg ttctgggcca tgaactgggc cacgtcgcgt
     5881 tgggtcactc gctggctgaa atgaaagctt cttatgcgat cgttgccgca cgcgatgcca
     5941 tttcagctac cagcggagtg gcttcccagc tttcccgctc acaattgggt gatatcgcag
     6001 aaggcgctat taatgctaaa tactcccgtg ataaagagtc cgaagcagat gatttctcct
     6061 ttgatctgtt gaagaaacgt ggcatcagca cccaggggct ggttggcagc tttgaaaaac
     6121 tggctagtct ggatggcggt cgcacccagt ccatgtttga ctctcaccca ccatcaacag
     6181 agcgtgcgca acacatccgt gatcgtatcg cctctggtaa gtaaatcatt gtcatctttc
     6241 gggctggtct tctgccagcc cgctataatt gcgcaataaa tccccatctg aatacagaca
     6301 aaactggttt ttgcacacaa cgttaacgat ttgtggcgtc ggcgcgtata atgcgcgcgg
     6361 ttatgttaac ggtacgcctg ttttaaggag ataaagatga cggcaattgc cccggttatt
     6421 accattgatg gcccaagcgg tgcagggaaa ggcaccttgt gtaaggctat ggcggaagca
     6481 ttgcaatggc atctgctgga ttcgggtgca atttatcgcg tactggcatt ggcggcatta
     6541 catcaccatg ttgatgttgc gtcagaagat gcgctggtac cgctggcatc ccatctggat
     6601 gtacgttttg tgtcaaccaa tggcaatctg gaagtgatcc tcgaagggga agatgtcagc
     6661 ggcgaaattc gtactcagga agtggcgaat gcagcttcac aagtcgcggc attcccacgc
     6721 gttcgtgaag cattattgcg tcgccaacgc gcgtttcgcg aattaccagg cctgattgcc
     6781 gatggccgcg acatgggaac ggtggtattc cctgatgcgc cagtgaaaat tttccttgac
     6841 gcctcctcgg aagaacgtgc gcatcgccgc atgctacagt tgcaggagaa gggctttagt
     6901 gttaactttg agcgcctttt ggccgagatc aaagaacgcg acgaccgcga tcgtaaccga
     6961 gcggtagcgc cactggttcc ggcagccgat gctttagtgt tggattccac caccttaagc
     7021 attgagcaag tgattgaaaa agcgctacaa tacgcgcgcc agaaattggc tctcgcataa
     7081 gcgaccgaat ttgcagtacc cccgttgcaa tggaatgaca gcgggtatgt taaacaaccc
     7141 catccggcat ggagccaggt ggacgttaaa tataaacctg aagattaaac atgactgaat
     7201 cttttgctca actctttgaa gagtccttaa aagaaatcga aacccgcccg ggttctatcg
     7261 ttcgtggcgt tgttgttgct atcgacaaag acgtagtact ggttgacgct ggtctgaaat
     7321 ctgagtccgc catcccggct gagcagttca aaaacgccca gggcgagctg gaaatccagg
     7381 taggtgacga agttgacgtt gctctggacg cagtagaaga cggcttcggt gaaactctgc
     7441 tgtcccgtga gaaagctaaa cgtcacgaag cctggatcac gctggaaaaa gcttacgaag
     7501 atgctgaaac tgttaccggt gttatcaacg gcaaagttaa gggcggcttc actgttgagc
     7561 tgaacggtat tcgtgcgttc ctgccaggtt ctctggtaga cgttcgtccg gtgcgtgaca
     7621 ctctgcacct ggaaggcaaa gagcttgaat ttaaagtaat caagctggat cagaagcgca
     7681 acaacgttgt tgtttctcgt cgtgccgtta tcgaatccga aaacagcgca gagcgcgatc
     7741 agctgctgga aaacctgcag gaaggcatgg aagttaaagg tatcgttaag aacctcactg
     7801 actacggtgc attcgttgat ctgggcggcg ttgacggcct gctgcacatc actgacatgg
     7861 cctggaaacg cgttaagcat ccgagcgaaa tcgtcaacgt gggcgacgaa atcactgtta
     7921 aagtgctgaa gttcgaccgc gaacgtaccc gtgtatccct gggcctgaaa cagctgggcg
     7981 aagatccgtg ggtagctatc gctaaacgtt atccggaagg taccaaactg actggtcgcg
     8041 tgaccaacct gaccgactac ggctgcttcg ttgaaatcga agaaggcgtt gaaggcctgg
     8101 tacacgtttc cgaaatggat tggaccaaca aaaacatcca cccgtccaaa gttgttaacg
     8161 ttggcgatgt agtggaagtt atggttctgg atatcgacga agaacgtcgt cgtatctccc
     8221 tgggtctgaa acagtgcaaa gctaacccgt ggcagcagtt cgcggaaacc cacaacaagg
     8281 gcgaccgtgt tgaaggtaaa atcaagtcta tcactgactt cggtatcttc atcggcctgg
     8341 acggcggcat cgacggcctg gttcacctgt ctgacatctc ctggaacgtt gcaggcgaag
     8401 aagcagttcg tgaatacaaa aaaggcgacg aaatcgctgc agttgttctg caggttgacg
     8461 cagaacgtga acgtatctcc ctgggcgtta aacagctcgc agaagatccg ttcaacaact
     8521 gggttgctct gaacaagaaa ggcgctatcg taaccggtaa agtaactgca gttgacgcta
     8581 aaggcgcaac cgtagaactg gctgacggcg ttgaaggtta cctgcgtgct tctgaagcat
     8641 cccgtgaccg cgttgaagac gctaccctgg ttctgagcgt tggcgacgaa gttgaagcta
     8701 aattcaccgg cgttgatcgt aaaaaccgcg caatcagcct gtctgttcgt gcgaaagacg
     8761 aagctgacga gaaagatgca atcgcaactg ttaacaaaca ggaagatgca aacttctcca
     8821 acaacgcaat ggctgaagct ttcaaagcag ctaaaggcga gtaattctct gactcttcgg
     8881 gatttttatt ccgaagtttg ttgagtttac ttgacagatt gcaggtttcg tcctgtaatc
     8941 aagcactaag ggcggctacg gccgccctta atcaatgcag caacagcagc cgcttaattt
     9001 gcctttaagg aaccggagga atcatgacca agtcagaatt gatagaaaga cttgccaccc
     9061 agcaatcgca cattcccgcc aagacggttg aagatgcagt aaaagagatg ctggagcata
     9121 tggcctcgac tcttgcgcag ggcgagcgta ttgaaatccg cggtttcggc agtttctctt
     9181 tgcactaccg cgcaccacgt accggacgta atccgaagac tggcgataaa gtagaactgg
     9241 aaggaaaata cgttcctcac tttaaaccag gtaaagaact gcgcgatcgc gccaatattt
     9301 acggttaagt ttttactcaa acttgaacgc gagaaaagca cctgtcgggt gcttttttca
     9361 tttctctaat caggaactgg aagctgcctc gcagagtttt gagcagtttt caccctctcg
     9421 ttaaattctt ctgaacgtgc ctcggggaac gcaaaattcc caccagacaa ccgctcaaca
     9481 aagttgcaca ctttccataa acagggaggg gtgcatgaaa ataacgacag tcggcgtatg
     9541 cataatttgc ggaatttttc cgttgctgat tttgccccaa ttgcctggga cattaaccct
     9601 tgcgtttctg actctcttcg cctgcgtact ggcatttatc cctgttaaaa ccgtccgtta
     9661 tatcgcgctg acgttgttgt ttttcgtttg gggcatatta gcagcaaagc aaattttgtg
     9721 ggcaggagaa accttaactg gcgcgacgca ggatgcaatt gttgagatca ctgctactga
     9781 cggtatgacc actcattacg gtcaaattac tcatctacaa ggtcgacgta tattccctgc
     9841 gccaagcctt gtgctgtatg gcgaatatct tccgcaagcg gtttgtgccg gacaactgtg
     9901 gtcaatgaaa ctcaaagttc gtgccgttca tggtcaactt aatgatggcg gctttgatag
     9961 ccagcgttat gccattgccc agcatcagcc gctcaccggc cgctttctgc aggcaagtgt
    10021 tattgaaccg aattgtagcc tgcgtgcaca gtatctggcg tcactacaaa caacgctgca
    10081 accctatccg tggaatgcgg ttattcttgg tttaggtatg ggggaacggt tatccgtccc
    10141 taaagaaatc aaaaatatca tgcgtgatac tggaacggcg catttaatgg cgatatcggg
    10201 attgcacatc gcttttgcgg cgttgctggc tgccggactc attcgcagtg gacaaatttt
    10261 tctgcctggg cgctggatcc actggcaaat gccattaatt ggcggaatct gctgtgctgc
    10321 tttttatgcc tggctgacgg gaatgcaacc tcctgcattg cgtaccgttg tggcgcttgc
    10381 tacgtgggga atgcttaagt taagtgggcg acagtggagt ggctgggatg tatggatatg
    10441 ttgtctggcg gcaattttgc tgatggatcc tgttgccatt ctctcgcaaa gtttatggct
    10501 ctctgccgct gcggtcgcgg cattgatatt ttggtatcag tggtttccct gtcctgagtg
    10561 gcaactgccg ccggtattgc gtgcagttgt ttccctcatc catctgcaac tgggaatcac
    10621 acttctgctt atgcccgtgc aaatcgtcat atttcatggc attagtctga cctcgtttat
    10681 tgcaaatcta ttagcaattc ccctggtgac atttatcacg gttccgttga tcctcgccgc
    10741 gatggttgtg catttaagcg ggccgttaat cctggagcaa gggttatggt ttcttgccga
    10801 ccggtctttg gctttacttt tctgggggtt aaagagtttg ccggaagggt ggatcaacat
    10861 tgctgaatgt tggcaatggc tatcattttc cccatggttc ttactggtgg tatggcgatt
    10921 aaacgcctgg cgaacgttgc cagcaatgtg tgtggctgga ggcttgctga tgtgctggcc
    10981 gctgtggcaa aaacctcgac ctgacgagtg gcagctgtac atgcttgatg tcgggcaagg
    11041 gctggcaatg gtgatagcca gaaacggcaa agcgattctc tatgacacag gactggcctg
    11101 gcccgaaggg gatagtgggc aacaactgat tatcccctgg ctccactggc ataatcttga
    11161 accggaaggc gttattctga gtcatgaaca tctggatcac cggggagggc tggactcaat
    11221 attgcacaca tggccgatgt tatggatcag aagtccgtta aactgggaac atcatcagcc
    11281 ctgtgtgcgt ggcgaagcgt ggcaatggca aggattgcgt ttcagcgtgc actggccttt
    11341 acaagctagc aacgataaag gaaataacca ttcctgtgtg gttaaggttg atgacgggac
    11401 gaatagcatt cttctaaccg gtgatattga agtccccgct gaacaaaaga tgctaagccg
    11461 ttactggcag caagtgcaga caacattgct tcaggtacct caccatggca gtaatacctc
    11521 atcatcgttg ccattaattc agcgagtgaa tggaaaagtg gcactcgcat cggcatcgcg
    11581 ctataacgca tggcgattgc cctctaataa agttaagcat cgctatcaac agcaaggata
    11641 tcaatggctt gatactccac atcagggtca agtgacggtc aatttttcag cgcaaggctg
    11701 gcggattagc agcctcagag agcaaatttt acctcgttgg tatcatcagt ggtttggcgt
    11761 gccagtggat aacgggtaga atatgcggct atttcaacaa atgctggttt tttgaatgca
    11821 taacgacaaa gatctctcta cgtggcagac gttccgccga ctgtggccaa ccattgcgcc
    11881 ttttaaagcg ggtctgatcg tggcgggcgt agcgttaatc ctcaacgcag ccagcgatac
    11941 cttcatgtta tcgctcctta agccacttct tgatgatggc tttggtaaaa cagatcgctc
    12001 cgtgctggtg tggatgccgc tggtggtgat cgggctgatg attttacgtg gtatcaccag
    12061 ctatgtctcc agctactgta tctcctgggt atcaggaaag gtggtaatga ccatgcgtcg
    12121 ccgcctgttt ggtcacatga tgggaatgcc ggtttcattt tttgacaaac agtcaacggg
    12181 gacgctgttg tcacgtatca cctacgattc cgaacaggtt gcttcttctt cttccggcgc
    12241 actgataacg gttgtgcgtg aaggtgcgtc aatcatcggc ctgttcatca tgatgttcta
    12301 ttacagttgg caactgtcga tcattttgat tgtgctggca ccgattgttt cgattgcgat
    12361 tcgcgtagta tcgaagcgtt ttcgcaacat cagtaaaaac atgcagaaca ccatggggca
    12421 ggtgaccacc agcgccgaac aaatgctgaa gggccacaaa gaagtattga ttttcggtgg
    12481 tcaggaagtg gaaacgaaac gctttgataa agtcagcaac cgaatgcgtc ttcaggggat
    12541 gaaaatggtt tcagcctctt ccatctctga tccgatcatt cagctgatcg cctctttggc
    12601 gctggcgttt gttctgtatg cggcgagctt cccaagtgtc atggatagcc tgactgccgg
    12661 tacgattacc gttgttttct cttcaatgat tgcactgatg cgtccgctga aatcgctgac
    12721 caacgttaac gcccagttcc agcgcggtat ggcggcttgt cagacgctgt ttaccattct
    12781 ggacagtgag caggagaaag atgaaggtaa gcgcgtgatc gagcgtgcga ctggcgacgt
    12841 ggaattccgc aatgtcacct ttacttatcc gggacgtgac gtacctgcat tgcgtaacat
    12901 caacctgaaa attccggcag ggaagacggt tgctctggtt ggacgctctg gttcgggtaa
    12961 atcaaccatc gccagcctga tcacgcgttt ttacgatatt gatgaaggcg aaatcctgat
    13021 ggatggtcac gatctgcgcg agtataccct ggcgtcgtta cgtaaccagg ttgctctggt
    13081 gtcgcagaat gtccatctgt ttaacgatac ggttgctaac aacattgctt acgcacggac
    13141 tgaacagtac agccgtgagc aaattgaaga agcggcgcgt atggcctacg ccatggactt
    13201 catcaataag atggataacg gtctcgatac agtgattggt gaaaacggcg tgctgctctc
    13261 tggcggtcag cgtcagcgta ttgctatcgc tcgagccttg ttgcgtgata gcccgattct
    13321 gattctggac gaagctacct cggctctgga taccgaatcc gaacgtgcga ttcaggcggc
    13381 actggatgag ttgcagaaaa accgtacctc tctggtgatt gcccaccgct tgtctaccat
    13441 tgaaaaggca gacgaaatcg tggtcgtcga ggatggtgtc attgtggaac gcggtacgca
    13501 taacgatttg cttgagcacc gcggcgttta cgcgcaactt cacaaaatgc agtttggcca
    13561 atgatcgaaa aaatctggtc tggtgaatcc cctttgtggc ggctattgct gccactctcc
    13621 tggttgtatg gcctggtgag tggcgcgatc cgtctttgct ataaactaaa actgaagcgc
    13681 gcctggcgtg cccccgtacc ggttgtcgtg gttggtaatc tcaccgcagg cggcaacgga
    13741 aaaaccccgg tcgttgtctg gctggtggaa cagttgcaac agcgcggtat tcgcgtgggg
    13801 gtcgtatcgc ggggatatgg tggtaaggct gaatcttatc cgctgttatt gtcggcagat
    13861 accacaacag cacaggcggg tgatgaacct gtgttgattt atcaacgcac tgatgcgcct
    13921 gttgcggttt ctcccgttcg ttctgatgcg gtaaaagcca ttctggcgca acaccctgat
    13981 gtgcagatca tcgtaaccga cgacggttta cagcattacc gtctggcgcg tgatgtggaa
    14041 attgtcgtta ttgatggtgt gcgtcgcttt ggcaatggct ggtggttgcc ggcggggcca
    14101 atgcgtgagc gagcggggcg cttaaagtcg gttgatgcgg taatcgtcaa cggcggtgtc
    14161 cctcgcagcg gtgaaatccc catgcatctg ctgccgggtc aggcggtgaa tttacgtacc
    14221 ggtacgcgtt gtgacgttgc tcagcttgaa catgtagtgg cgatggcggg gattgggcat
    14281 ccgccgcgct tttttgccac gctgaagatg tgtggcgtac aaccggaaaa atgtgtaccg
    14341 ctggccgatc atcagtcttt gaaccatgcg gatgtcagtg cgttggtaag cgccgggcaa
    14401 acgctggtaa tgactgaaaa agatgcggtg aaatgccggg cctttgcaga agaaaattgg
    14461 tggtatttgc ctgtagacgc acagctttca ggtgatgaac cagcgaaact gcttacgcaa
    14521 ctaacctcgc tggcttctgg caactagtta cgccgcggca gcgttcgatt gatggagtca
    14581 tgaatgtcgc tgccgcacct ctcccttgct gatgcgcgta atcttcacct tgccgcacaa
    14641 ggcctgttaa acaaaccccg ccgtcgagcg tcgttggagg atattccggc aacgatctcc
    14701 cgcatgtcct tgctgcaaat cgataccatc aatattgttg cccgtagtcc atatctggtg
    14761 cttttcagtc gtctgggaaa ttatcctgcc cagtggctgg atgagtctct ggcgcgtggc
    14821 gaattaatgg aatactgggc gcatgaagcc tgctttatgc cgcgtagcga ctttcgtctt
    14881 attcgccacc gcatgctggc acctgaaaaa atgggctgga aatacaaaga cgcctggatg
    14941 caggaacatg aggcggaaat tgcacagtta attcagcata ttcatgataa ggggccggta
    15001 cgttcagccg attttgagca tcctcgtaaa ggtgcaagcg gctggtggga atggaagccg
    15061 cataaacggc atctggaagg tttatttact gccggaaagg tgatggtgat tgaacggcgc
    15121 aacttccagc gcgtttatga tttaacccac cgtgtcatgc ctgactggga tgatgagcgc
    15181 gatctcgttt cgcaaacaga agcagaaatc atcatgctgg ataacagtgc gcgtagcctg
    15241 ggaatattcc gcgaacagtg gctggcagat tactatcggc tgaaacgtcc ggcactggcg
    15301 gcgtggcgcg aagcgagggc tgaacagcag caaatcattg ctgtgcatgt tgaaaaattg
    15361 ggcaatcttt ggctgcatgc tgatttgctg ccgctactcg agcgtgcgct ggccggaaag
    15421 ctcactgcaa cgcacagcgc ggtactttcg ccttttgatc ctgttgtctg ggatcgcaaa
    15481 cgcgcagagc agctttttga ttttagctac cggctggagt gctatacccc agcgccgaaa
    15541 cgccagtatg gctattttgt tctgccatta ttacatcgtg ggcaattagt tgggcgaatg
    15601 gatgccaaaa tgcatcgcca gacaggcatc cttgaagtta tctctctgtg gttacaggaa
    15661 ggcattaaac caacgacaat gctgcaaaaa gggttacgtc aggcgattac tgatttcgcg
    15721 agctggcagc aggcaacgcg ggtgacatta ggacgctgcc cgcaaggtct ctttactgat
    15781 tgccgcactg gctgggaaat agaccccgtc gcataaatga ttatgataag atttatgcat
    15841 tcataacccg atccatctgg aggaactatg gatcatcgtt tgcttgaaat cattgcctgc
    15901 ccggtttgca acggaaaact ttggtataac caggaaaaac aagagcttat ttgcaaactg
    15961 gataacctgg ctttcccgct ccgcgatggc attccagtgt tgctggaaac ggaagcccgc
    16021 gtgctgactg ctgatgagag taaatcatga gttttgtggt cattattccc gcgcgctacg
    16081 cgtccacgcg tctgcccggt aaaccattgg ttgatattaa cggcaaaccc atgattgttc
    16141 atgttcttga acgcgcgcgt gaatcaggtg ccgagcgcat catcgtggca accgatcatg
    16201 aggatgttgc ccgcgccgtt gaagccgctg gcggtgaagt atgtatgacg cgcgccgatc
    16261 atcagtcagg aacagaacgt ctggcggaag ttgtcgaaaa atgcgcattc agcgacgaca
    16321 cggtgatcgt taatgtgcag ggtgatgaac cgatgatccc tgcgacaatc attcgtcagg
    16381 ttgctgataa cctcgctcag cgtcaggtgg gtatggcgac tctggcggtg ccaatccgca
    16441 atgcggaaga agcgtttaac ccgaatgcgg tgaaagtggt tctcgacgct gaagggtatg
    16501 cactgtactt ctcacgcgcc accattcctt gggatcgtga tcgttttgca gaaggccttg
    16561 aaaccgttgg cgataacttc ctgcgtcatc ttggtattta tggctaccgt gcaggcttta
    16621 tccgtcgtta cgtcaactgg caggcaagtc cgttagaaca catcgaaatg ttagagcagc
    16681 ttcgtgttct gtggtacggc gaaaaaatcc atgttgctgt tgcgcatgaa gttcctggca
    16741 caggtgtgga tacccctgaa gatctcgagc gcgttcgcgc tgaaatgcgc taatttcact
    16801 tcacgacact tcagccaatt ttgggaggag tgtcgtaccg ttacgatttt cctcaatttt
    16861 tcttttcaac aattgatctc attcaggtga catcttttat attggcgctc attatgaaag
    16921 cagtagcttt tatgagggta atctgaatgg aacagctgcg tgccgaatta agccatttac
    16981 tgggcgaaaa actcagtcgt attgagtgcg tcaatgaaaa agcggatacg gcgttgtggg
    17041 ctttgtatga cagccaggga aacccaatgc cgttaatggc aagaagcttt agtacgcccg
    17101 gaaaagcccg acaactggca tggaaaacca ccatgctggc aagaagtggg actgtccgta
    17161 tgccgactat ttatggtgtg atgacgcatg aagaacaccc cggccccgat gtcctgctac
    17221 tggagcggat gcgtggtgtt tcggtggagg caccagcccg aacaccagaa cgctgggaac
    17281 aactcaaaga ccaaatcgtt gaagccttac tggcctggca ccgtcaggac agtcgcggtt
    17341 gcgtcggcgc ggtcgacaat actcaggaaa atttctggcc ctcatggtac cggcaacatg
    17401 ttgaagtgct atggaccacg ctcaatcagt tcaataacac cggtctgacg atgcaggata
    17461 agcggatcct gtttcgcact cgcgaatgtc tcccggcatt atttgaaggc tttaacgaca
    17521 attgtgtgtt gattcacggt aacttctgtt tacgcagcat gctgaaagat tcgcgcagcg
    17581 atcagttact ggcgatggtc gggccgggac taatgctttg ggcaccgcga gaatacgaac
    17641 tgttccgact aatggataat tctctggcgg aagatttgct ctggagttac ctgcaacgcg
    17701 cgccagtggc ggagtcgttc atctggcggc gttggttgta tgtgttatgg gatgaagttg
    17761 cgcaactggt taataccgga cgatttagtc ggcgcaactt cgatctggca tcaaaatcac
    17821 tcttgccgtg gctcgcctga cggacctttc agccactgcc agatgcggcc aagcgtttca
    17881 tagccaacac gatcgctatg catcagccag accggagaag ggattgcccg ttcccacgga
    17941 ttaagcggcg agtcgatggc caactgattc gccggggctg gtagcggatt taacccttcc
    18001 tgctgaaaaa agatcatcgc ccgcggcaga tgtgatgcgg aggtcaccag caggaaaggg
    18061 gcgtcaccaa tcgcctgttt cactgccgca gcttcttctt cggtatcttt tggcaaatcc
    18121 agggtgataa tttgctcgcg cggcacgccc agcgattgcg caactctggc acctacttcc
    18181 gctgtactca ccgtattggt ttttgctacg cctcccgtga agatcagttt tgatcccgga
    18241 ttttcgcgcc ataagcgaat accttcattc aggcgtggca gactgttatt gattaaatta
    18301 gagctcggtg cccactgtgg gttccaggta taaccacctc cgagcaccac gatatagtcc
    18361 actttttgcg aattattcca tgtcggatag gtgctttcga tgggacgcag caggcggtcg
    18421 gctaccggtt gcaaactcaa taacaagagt gccagccacc caatgctaat aaaaatcttc
    18481 ccggtttttt gaaaacggct aaaccacagc agcgccaggc cagcgccaat aattaacaac
    18541 atcagcggaa gtggcagcaa catattgcca atcacttttt tcagtgtaaa aagcatcctt
    18601 tctggttcct tttttaacca tatagcaagg gatcgccagc gatattacac cagacgggtt
    18661 cattctcgcc gtggctgtga caaaatagcg tctttatcag aaaacgtctt agccaaccgg
    18721 tggagaaagc aatgcaggat cgcaatttcg atgatattgc ggaaaagttt tcccgtaaca
    18781 tttacggcac caccaaaggg cagcttcgac aggctattct gtggcaggat ctcgatcacg
    18841 tgctggcgga aatgggcccg caaaaactgc gtgtgctgga tgctggcggt ggagaagggc
    18901 agaccgcaat caaaatggcc gagcgtgggc atcaggtcat tttatgcgat ctttctgcgc
    18961 agatgatcga ccgcgcaaaa caggcggcag aagcaaaagg tgtgagcgac aacatgcaat
    19021 ttatacattg cgccgctcag gatgttgctt cgcatttgga aacgcccgtt gatctgatat
    19081 tgttccatgc ggtgctcgag tgggtggccg atccccgcag tgtattgcag accctgtggt
    19141 cagtgttgcg tccaggcgga gtgttgtcgt taatgttcta caatgcgcat ggtttgttga
    19201 tgcataacat ggtcgccggg aattttgatt acgtgcaggc gggaatgccg aaaaagaaaa
    19261 aacggacgct ttcgccagat tatccacgcg acccggcgca ggtttatctg tggctggaag
    19321 aagctggttg gcaaattatg ggtaagacag gcgttcgcgt gtttcatgat tatctgcgcg
    19381 agaagcacca gcagcgcgac tgctatgaag cattacttga attagaaacg cgttattgcc
    19441 gtcaggaacc gtatattacc ctggggcgtt atattcatgt caccgcgcgc aaaccgcaga
    19501 gcaaggataa agtatgagtg aattttccca gacagtcccc gaactggttg cctgggccag
    19561 aaaaaatgac ttctccatct cgctgccggt agaccgactc tcttttctgc tggcggttgc
    19621 cacgctgaac ggcgagcgtc tggatggtga gatgagtgaa ggcgagctgg tggatgcatt
    19681 ccgccatgtg agtgatgcgt ttgagcaaac cagcgaaacc atcggcgtgc gcgccaacaa
    19741 cgcgatcaac gacatggtgc gtcaacgtct gctgaaccgc tttaccagcg agcaggcgga
    19801 agggaacgca atttaccgtc tgacgccgct cggcatcggc atcactgact attacattcg
    19861 tcagcgcgag ttttctacgc tgcgcctttc tatgcagttg tcgattgtgg cgggtgagct
    19921 caaacgcgca gcagatgccg ccgaagaggg cggtgatgaa tttcactggc accgtaatgt
    19981 ttatgctcca ctgaaatatt cggtagcaga aattttcgac agtatcgacc tgacgcaacg
    20041 tctgatggat gaacagcagc agcaggtgaa ggatgatatc gcccagttgc tgaacaaaga
    20101 ctggcgggcg gcgatttcca gctgtgaatt gttgctttcg gaaacttccg gaacgctgcg
    20161 tgaattgcag gatacgctgg aagcggcagg cgacaaattg caggctaatc tgttgcgcat
    20221 tcaggatgcg acgatgaccc atgacgatct gcatttcgtc gatcgtctgg tgttcgatct
    20281 gcagagcaaa ctcgatcgta ttatcagttg gggccagcaa tccatcgact tgtggattgg
    20341 ctacgaccgc cacgtacaca aatttattcg taccgcgatc gatatggata aaaaccgcgt
    20401 ctttgctcag cggttacgtc agtcggtaca aacctatttt gatgagccgt gggcgctaac
    20461 ttatgccaat gccgatcgtc tgctggatat gcgtgacgaa gagatggcac tgcgcgatga
    20521 agaagtgact ggggaacttc ctgaggatct ggaatacgaa gagtttaacg agatccgcga
    20581 acagctggcg gcgatcatcg aagaacaact tgccgtgtac aaaaccagac aagtgccgct
    20641 ggatcttggt ctggtggtac gcgaatatct gtcacagtat ccgcgtgcac gtcactttga
    20701 cgttgcgcgt attgttattg atcaggcggt acgtcttggc gtagcgcaag cagatttcac
    20761 cggactgcca gcgaaatggc agccgattaa tgattacgga gccaaggtac aggcgcatgt
    20821 catcgacaaa tattgaacaa gtgatgccgg ttaagctggc gcaggcgctg gcgaatccgt
    20881 tatttccggc gctggacagc gccttacgtt caggccgcca tattggcctc gacgaactgg
    20941 ataatcatgc attcctgatg gattttcagg aatatctgga agagttttac gcgcgttata
    21001 acgttgagct tattcgcgca ccagaagggt tcttctattt acgcccacgt tccaccacgc
    21061 tgatccctcg ttccgtcttg tcggaactgg atatgatggt cgggaaaatc ctctgttatc
    21121 tctatctcag cccggaacgg ctggcgaatg aggggatttt cacccagcag gaactgtacg
    21181 acgaactgct caccctggcc gatgaagcaa aactgctgaa actggtgaac aaccgttcaa
    21241 ccggttcaga cgttgaccgt cagaagttgc aggagaaagt acgttcttcg ctcaaccgtc
    21301 tgcgtcgttt aggcatggtg tggtttatgg gccacgacag cagcaagttt cgcattaccg
    21361 aatcggtgtt ccgcttcgga gccgatgtgc gtgctggcga cgatccccgt gaagcacagc
    21421 gtcgcctgat tcgtgatggc gaagcaatgc cgattgaaaa tcatctgcaa ctcaacgatg
    21481 aaaccgaaga gaatcagcca gatagcggag aggaagaata atgattgaac gcggtaaatt
    21541 tcgctcactg acgctgatta actggaacgg cttttttgcc cgaacttttg accttgacga
    21601 gctggtcacg acgctttctg gcggtaacgg ggcgggtaaa tccaccacca tggcggcgtt
    21661 cgttacggcg ctgatccccg acctgaccct gctgcatttc cgtaacacta cggaagccgg
    21721 ggccaccagc ggttcgcgcg ataaaggtct gcacggtaag ctgaaagcgg gtgtctgtta
    21781 ttcgatgctc gacaccatta actcgcgcca ccagcgcgtg gtggtcggtg tgcgtctgca
    21841 acaggttgcc ggacgcgatc gtaaagtgga tatcaaacca tttgccattc agggactgcc
    21901 gatgtcggtg cagccgacac agctggtgac cgaaaccctg aacgaacgcc aggcgcgcgt
    21961 gctgccgctt aacgagctga aagacaagct cgaggcgatg gaaggcgtgc agtttaaaca
    22021 gttcaactcc attaccgatt accactcgct gatgttcgat cttggcatca tcgcgcgtcg
    22081 tctgcgctcc gcatctgacc gtagcaaatt ctatcgtctg atcgaagctt cgctgtatgg
    22141 cgggatctcc agtgccatta cccgttctct gcgcgactac ctgttgccgg aaaacagcgg
    22201 cgtgcgtaaa gcgttccagg acatggaagc ggcgctgcgt gaaaaccgca tgacgctgga
    22261 agcgattcgt gtcacccagt cggaccgtga cctgtttaag catctgatca gcgaagctac
    22321 caactacgtg gcggcggact acatgcgtca cgccaacgag cgccgtgtcc atctcgacaa
    22381 agccctggag tttcgtcgcg agttacatac ttcgcgtcag caactggcgg ctgagcagta
    22441 caaacacgtc gatatggcgc gtgagctggc agagcacaac ggtgccgaag gcgatctgga
    22501 agcggattat caggcggcca gcgatcacct gaacctggtg caaaccgcac tgcgtcagca
    22561 ggagaagatc gaacgctacg aagcggatct cgatgagctg cagatccgtc tggaagagca
    22621 aaacgaagtg gtggcagaag ccatcgaacg ccaggaagag aacgaggctc gcgcggaagc
    22681 tgccgaactg gaagtggacg agctgaaaag ccagcttgct gactaccagc aggcgctgga
    22741 cgtccagcaa acgcgcgcga tccagtataa ccaggcgatt gctgcgctta atcgcgccaa
    22801 agaactgtgc catctgccgg acttaacctc cgacagcgcc gccgaatggc tggaaacctt
    22861 ccaggcgaaa gagctggaag cgactgaaaa aatgctctct cttgagcaga aaatgagcat
    22921 ggcgcaaacc gcgcacagcc agtttgagca ggcttatcag ctggtggtgg caatcaacgg
    22981 cccgctggcg cgtaacgagg cgtgggatgt cgctcgcgaa ctattgcgcg aaggggtcga
    23041 tcagcgtcac ctggcagagc aggttcagcc gttgcggatg cgcttaagcg aactggaaca
    23101 gcgtctgcgc gaacagcaag aagctgagcg tctgctggca gatttctgca aacgtcaggg
    23161 caagaatttt gacattgacg agctggaagc tctgcatcag gagctggaag cgcgcattgc
    23221 ctctctttcc gatagcgtgt ctaacgcccg tgaagagcgc atggcactgc gccaggaaca
    23281 ggaacagctg cagtctcgca ttcagagttt gatgcagcgt gcgccggttt ggctggcagc
    23341 gcaaaacagt ctcaaccagt tgagcgaaca gtgcggcgaa gagtttacct ccagccagga
    23401 cgtcaccgaa tatctgcaac agttgctgga gcgtgagcga gaggcgattg ttgaacgcga
    23461 tgaagtgggc gcgcgcaaaa acgccgtcga tgaagagatc gaacgtttaa gccagcctgg
    23521 cggctctgaa gatcagcgtc tgaacgcgct ggcggagcgt tttggtggtg tgctgctgtc
    23581 agaaatttat gacgacgtta gcctggaaga tgcgccgtac ttctcagcgc tgtatggccc
    23641 gtcacgccac gccatcgtgg tgccagatct gtcacaggta actgaacacc tggaaggctt
    23701 gaccgattgc ccggaagatc tctatctgat cgaaggagat ccgcagtcat tcgatgacag
    23761 cgtgttcagc gttgatgagc tggaaaaagc ggtagtggtg aaaatcgccg atcgtcagtg
    23821 gcgttattca cgtttcccgg aagtgccgct gtttggtcgt gctgcgcgtg aaagccgtat
    23881 tgaaagcctc catgccgagc gtgaagtgct ttccgaacgc ttcgccacgc tctcctttga
    23941 tgtacagaaa actcagcgtc tgcatcaggc gttcagccgc tttatcggca gtcatctggc
    24001 ggttgcgttt gagtctgacc cggaagcaga aatccgtcaa ctgaacagcc gtcgcgtcga
    24061 actggagcgg gcgttgagta atcatgaaaa tgataaccag cagcagcgta ttcagtttga
    24121 gcaggcgaaa gaaggcgtta cggcgctgaa ccgcattctg ccgcgtctca acctgttggc
    24181 tgatgacagc ctggcggatc gcgtcgatga aatccgcgaa cgtctggatg aagcccagga
    24241 agccgcgcgt tttgttcagc agtttggcaa tcaactggcg aaactggaac cgatcgtttc
    24301 ggtattgcag agcgacccgg aacagttcga acagctaaaa gaagattacg cgtactctca
    24361 gcagatgcag cgcgatgccc gtcagcaggc gtttgccctg acggaagtgg tgcagcgtcg
    24421 tgcgcacttt agctattctg actcggcaga aatgctgagc ggtaatagcg atctcaacga
    24481 aaagctgcgt gaacgtctgg aacaggcgga agcggagcgt actcgcgctc gtgaagcgtt
    24541 gcgtggtcac gcagcgcagt tgagtcagta caaccaggtg ctggcttcgc tgaaaagttc
    24601 ttacgacacc aaaaaagagc tactcaacga tctgcaacgt gaattgcagg atatcggcgt
    24661 gcgtgctgat agcggggcag aagagcgggc gcgtattcgc cgtgacgagc tgcatgcgca
    24721 actgagcaat aaccgttcac gccgcaatca actggaaaaa gcgcttacct tctgcgaagc
    24781 ggagatggac aacctgaccc gcaaactgcg caagctggag cgggattact ttgagatgcg
    24841 cgagcaggta gtgaccgcca aagcgggctg gtgtgcggtg atgcgcatgg tgaaagataa
    24901 cggcgttgag cgccgcttac accgtcgtga gctggcttat ctctccgctg atgatttgcg
    24961 ctctatgtcg gataaggcat tgggtgcgct gcgtctggcg gtggcggata acgaacatct
    25021 gcgcgacgtg ctgcgcatgt cggaagatcc gaaacgtccg gagcgtaaaa ttcagttctt
    25081 cgtggcggtt tatcagcatc tgcgtgaacg tattcgtcag gatattattc gtaccgatga
    25141 tccggtggaa gctatcgaac agatggagat cgaacttagc cgtctgaccg aagaattaac
    25201 ctcccgtgaa cagaaactgg cgatcagttc ccgcagcgtg gcgaacatca ttcgcaaaac
    25261 cattcagcgc gagcagaacc gtatccgtat gctcaaccag gggttgcaga acgtatcgtt
    25321 tggtcaggtg aacagcgtgc gcctcaacgt gaacgtgcgt gaaacgcacg ccatgctgct
    25381 ggatgtgctc tctgaacagc acgagcagca tcaggatctg tttaacagca accgtctgac
    25441 cttctcggaa gcgttggcga aattgtatca gcgtcttaat ccgcagattg atatgggaca
    25501 gcgcacgccg cagaccattg gcgaagaact gctggattac cgcaactatc tggaaatgga
    25561 agttgaggtt aaccgtggtt ccgatggttg gttgcgcgca gagtctggtg cattgtcgac
    25621 cggtgaggcg attggtaccg gtatgtcgat tctggtgatg gtggtacaaa gctgggaaga
    25681 tgaatctcgc cgcctgcgcg gtaaagatat ctctccttgc cgcctgctgt tcctcgatga
    25741 agcagcgcga ctggatgctc gttctatcgc cacgctgttt gaattgtgtg agcgtttgca
    25801 aatgcagctc atcatcgcag cgccggaaaa tatcagcccg gagaaaggca ccacctataa
    25861 actggtgcgt aaagtcttcc agaataccga acacgttcac gtcgtcggcc tgcgaggatt
    25921 tgcgccgcaa ctccctgaaa cgcttccagg aagtgacgaa gcgccttctc aggcgagtta
    25981 aaattaaggc ggcagcaatg ccgccttttc tttttccgaa aactccgttt ccgcactaaa
    26041 aaagtggcac attacggcgc gcttttcttt aaacttcttt acattaggtt atgtaaaaac
    26101 gtatcggcgt ttatatactg aagataagcc tgatgagtaa caggcttgct cgtcatactt
    26161 tcgtgagtat tggcgttgta caggcaagtc gtaaaataac agcctggcta ttcagagtat
    26221 gataaaaaca gggggcaagg gatgttgctt aatatgatgt gtggtcgtcg gctgtcggca
    26281 atcagtttgt gcctggccgt aacattcgct ccactgttca atgcgcaggc cgatgagcct
    26341 gaagtaatcc ctggcgacag cccggtggct gtcagtgaac agggcgaggc actgccgcag
    26401 gcgcaagcca cggcaataat ggcggggatc cagccattgc ctgaaggtgc ggcagaaaaa
    26461 gcccgcacgc aaatcgaatc tcaattaccc gcaggttaca agccggttta tcttaaccag
    26521 cttcaactgt tgtatgccgc acgcgatatg caacccatgt gggaaaaccg tgatgctgtt
    26581 aaagccttcc agcaacagct ggcagaggtg gcgattgccg gtttccagcc gcagtttaat
    26641 aaatgggtag agttactgac cgatcctggt gttaacggga tggcacgcga cgtagtgctc
    26701 tctgatgcga tgatgggcta tctccatttc attgcaaata ttccggtcaa aggcactcgc
    26761 tggctatata gcagtaaacc ttatgcactt gcaacgccgc cgctctcggt gattaaccaa
    26821 tggcagctgg cgctggataa aggtcaattg cctacgtttg ttgcaggact ggcaccgcag
    26881 catccgcaat atgcggcgat gcatgaatcg ttactggcct tactcagtga caccaaaccg
    26941 tggccccaac tgaccggcaa agcaacgttg cgcccagggc agtggagtaa cgacgtaccg
    27001 gcgttgcgcg aaatattgca acgcacaggc atgttggacg gggggccgaa aattactcta
    27061 cctggcgatg acacgccaac tgacgcggta gtcagcccat ccgctgttac tgttgaaaca
    27121 gcagaaacta agccgatgga caagcaaacg acgtctcgta gtaaacctgc gcctgccgtt
    27181 cgtgccgcct acgataatga actggtggaa gccgttaaac gttttcaggc atggcaagga
    27241 ttgggggcag atggtgctat tggcccggca acacgtgact ggttaaacgt aacgcccgcc
    27301 cagcgtgctg gcgtgttggc tctcaacatc cagcgattgc gcttgctgcc aacagagctt
    27361 tctaccggga tcatggttaa cattccggcc tattcgctgg tctactatca gaacggcaat
    27421 caggtgctgg attcgcgagt cattgtcggt cgccccgatc gcaaaacgcc gatgatgagc
    27481 agtgccctta acaacgtagt ggtaaacccg ccgtggaacg tacctccaac tctggcacgc
    27541 aaagatattc tgccaaaagt gcgcaacgat ccgggatatc tcgaaagcca tggctatacg
    27601 gtgatgcgcg gctggaacag cagagaagcg attgatccat ggcaggttga ctggtctaca
    27661 atcacggcct cgaatttacc gttccgcttc cagcaggctc caggcccacg gaactcgctg
    27721 gggcgctata aattcaatat gccgagttca gaggccattt atttgcatga cacgccgaac
    27781 cacaatctgt tcaagcgtga tacacgcgca ttgagctcag gctgtgtacg agtgaataaa
    27841 gcttccgatc tggcgaatat gctgttgcag gatgcaggct ggaatgacaa acgtatttct
    27901 gatgcgctga agcagggtga tacacgttac gtcaatattc ggcagtcgat tccggtgaat
    27961 ctctactacc tgacggcctt tgttggtgca gatggtcgta cccagtatcg tacagatatt
    28021 tacaattatg atctgcctgc gcgatccagc tcgcaaatcg tatcgaaagc ggaacaatta
    28081 atcaggtaaa tgaagtagtt cggggaaatc agttgtcgta ataattagca tgattggggg
    28141 cgattctctg cagcccccgt cactgctggg gttgagtcat cttgacgtct gctttacggg
    28201 cggttaaggt gcctcttgtg cgccagaagt gcatataaac gataacattg acctgtagac
    28261 ttgattatca tggacaaatt cgacgctaat cgccgcaaat tgctggcgct tggtggcgtt
    28321 gcactcggtg ccgccatcct gccgacccct gcgtttgcaa cactctctac cccacgcccg
    28381 cgcattttga cactcaataa tcttcatacc ggagagtcaa tcaaagcgga gtttttcgat
    28441 ggcagaggct atattcagga agaattggca aaacttaacc attttttccg cgattaccgc
    28501 gcgaacaaaa taaagtccat cgacccagga ttattcgacc agttgtatcg cctgcaaggg
    28561 ttgttaggca cgcgcaaacc ggtgcaactc atttccggtt atcgttccat tgataccaac
    28621 aatgaactac gcgcccgcag ccgtggagta gcgaagaaaa gctatcacac taaaggccag
    28681 gcgatggatt tccatattga aggtatcgcg ttaagcaata ttcgcaaagc cgcgttatct
    28741 atgcgcgcag gtggtgtagg atattaccca cgtagtaact ttgtgcatat tgataccggg
    28801 ccagcacggc actggtagta attgcttaac gaaacagggg cagtatgaac tatcgtatta
    28861 ttccggtcac cgcattctcc cagaactgtt cattgatctg gtgtgaacaa acccgcctgg
    28921 ccgcactggt cgatcctggc ggcgatgcgg aaaaaatcaa acaggaagtt gatgacagcg
    28981 gcctgacact gatgcagatc ctgctgacgc atggtcatct ggaccacgtt ggtgcagcgg
    29041 cggaactggc gcaacattac ggcgtgccgg tttttggtcc ggaaaaagaa gatgagttct
    29101 ggctgcaagg cttgcctgcg caaagtcgta tgtttggtct ggaagagtgc cagccgctga
    29161 cgccagatcg ttggctgaac gaaggcgata ccatcagcat agggaatgtg actttacagg
    29221 tgttacattg ccctgggcat acgccgggtc atgtcgtgtt ttttgatgat cgggcaaagc
    29281 tgctgatttc tggcgatgtt attttcaaag gcggagtagg gcgcagtgac ttcccgcgtg
    29341 gcgatcataa tcaactgatt tcttcaatca aagataaatt gctgccactg ggggatgacg
    29401 tgacatttat tccgggtcac ggaccattat ccacacttgg ttatgaacgc ctgcataatc
    29461 ccttcctgca agacgaaatg cccgtctggt aaggcacata aaaaagcccg cttttaatgc
    29521 gggcctggat ttctgacaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat
    29581 ccggcctaca aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg
    29641 tagcgcatca ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca
    29701 ttgtttttaa tgcttacagc actgccacaa tcgcttcgca cagcggagcc atgttatctg
    29761 gtgtcatccc ggccacattt acgcgaccag aagcaaccgc atatacgcca aactcttcgc
    29821 gcagacgcag cacttgttct tttgtcaggc cactgaagga gaacatgccg ttctgtttga
    29881 tgataaagct gaagtcgcgg tttgcgcctt tttcctgcag cgtattgacg aacaactgac
    29941 gcatacgctg aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaacg
    30001 catcgttgct caggatggtg gcaacaacag aagcgccgtg tgctggtggg ttagagtagt
    30061 tagcgcgaat cgccgctttc atttggctga atgcgcgatc aacggtttca ctgtcggcag
    30121 caaccagagt acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac
    30181 tggcaacaat cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt
    30241 ccagaccacg ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa
    30301 ctgagagttg tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat
    30361 ggcagcagcc atggaacagc actacgtcgc cagcctgagc ttcgttcagg ctgttaatca
    30421 gtgcatcgaa gtcaagagtg tgattttccg catcataata agcgtattca cgaacttcca
    30481 gacttgcaga gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacactc
    30541 gcttaacgct ggtatttttt gccaggaaat cggcagctac gcgtagtgcg ccagtgcccc
    30601 ccggagtctg tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca
    30661 gcagttcctg agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg
    30721 tggtttcatt ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt
    30781 tgcccgtctc atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg
    30841 cacgaaacag atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa
    30901 acatgacgag gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat
    30961 ttacaaaatt ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg
    31021 ctctggaagg tataaaaaaa acaggaccaa agtcctgttt tttcggcatt taacaaagag
    31081 gtgtgctatt agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc
    31141 agtttgttgt cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg
    31201 ttgaagtagt aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct
    31261 tctacgtctt tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac
    31321 tggtattgcg caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta
    31381 aatttattag tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caagtagatg
    31441 ttgttcgcgt cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga
    31501 ggttgagctt cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag
    31561 ccttcgtatt cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg
    31621 tcacgctcgt ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag
    31681 aagttggagt tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg
    31741 tatgcagtat caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc
    31801 acaccgtagt tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat
    31861 gccagacgcg ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag
    31921 ttatattccc actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa
    31981 ccaagacggg cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg
    32041 gaaaaataat gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta
    32101 tagatttctg cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga
    32161 atattgcgct tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc
    32221 cgtcaataag ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta
    32281 tgtgtctgca ggcatctttc cattcaaa
//
LOCUS       DAPHJE010000044        32010 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPHJE010000044 DAPHJE010000000
VERSION     DAPHJE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.044"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            114..557
                     /gene="cmtB"
                     /locus_tag="RH222_002090"
     CDS             114..557
                     /gene="cmtB"
                     /locus_tag="RH222_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV3797108.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFSGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            585..1973
                     /gene="cmtA"
                     /locus_tag="RH222_002091"
     CDS             585..1973
                     /gene="cmtA"
                     /locus_tag="RH222_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV3797109.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1988..3265
                     /locus_tag="RH222_002092"
                     /pseudo
     CDS             1988..3265
                     /locus_tag="RH222_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            3262..4227
                     /gene="yggF"
                     /locus_tag="RH222_002093"
     CDS             3262..4227
                     /gene="yggF"
                     /locus_tag="RH222_002093"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV3797110.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4249..4758
                     /gene="fumE"
                     /locus_tag="RH222_002094"
     CDS             4249..4758
                     /gene="fumE"
                     /locus_tag="RH222_002094"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV3797111.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4755..5468
                     /locus_tag="RH222_002095"
     CDS             4755..5468
                     /locus_tag="RH222_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV3797112.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5440..6117)
                     /locus_tag="RH222_002096"
     CDS             complement(5440..6117)
                     /locus_tag="RH222_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3797113.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6111..6788)
                     /locus_tag="RH222_002097"
     CDS             complement(6111..6788)
                     /locus_tag="RH222_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDV3797114.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6776..7483)
                     /locus_tag="RH222_002098"
     CDS             complement(6776..7483)
                     /locus_tag="RH222_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV3797115.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7484..8062)
                     /locus_tag="RH222_002099"
     CDS             complement(7484..8062)
                     /locus_tag="RH222_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV3797116.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8085..8516)
                     /locus_tag="RH222_002100"
     CDS             complement(8085..8516)
                     /locus_tag="RH222_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV3797117.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8888..9907
                     /gene="epd"
                     /locus_tag="RH222_002101"
     CDS             8888..9907
                     /gene="epd"
                     /locus_tag="RH222_002101"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV3797118.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9957..11120
                     /gene="pgk"
                     /locus_tag="RH222_002102"
     CDS             9957..11120
                     /gene="pgk"
                     /locus_tag="RH222_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV3797119.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11335..12414
                     /gene="fbaA"
                     /locus_tag="RH222_002103"
     CDS             11335..12414
                     /gene="fbaA"
                     /locus_tag="RH222_002103"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV3797120.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12772..13632
                     /gene="mscS"
                     /locus_tag="RH222_002104"
     CDS             12772..13632
                     /gene="mscS"
                     /locus_tag="RH222_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV3797121.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13771..14406
                     /gene="argO"
                     /locus_tag="RH222_002105"
     CDS             13771..14406
                     /gene="argO"
                     /locus_tag="RH222_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV3797122.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14499..15239
                     /locus_tag="RH222_002106"
     CDS             14499..15239
                     /locus_tag="RH222_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV3797123.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15406..16302
                     /locus_tag="RH222_002107"
     CDS             15406..16302
                     /locus_tag="RH222_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3797124.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEGRQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHKPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16299..17777)
                     /gene="scpC"
                     /locus_tag="RH222_002108"
     CDS             complement(16299..17777)
                     /gene="scpC"
                     /locus_tag="RH222_002108"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV3797125.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYQDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17801..18586)
                     /gene="scpB"
                     /locus_tag="RH222_002109"
     CDS             complement(17801..18586)
                     /gene="scpB"
                     /locus_tag="RH222_002109"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV3797126.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18597..19592)
                     /gene="meaB"
                     /locus_tag="RH222_002110"
     CDS             complement(18597..19592)
                     /gene="meaB"
                     /locus_tag="RH222_002110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV3797127.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DTIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIINFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19585..21729)
                     /gene="scpA"
                     /locus_tag="RH222_002111"
     CDS             complement(19585..21729)
                     /gene="scpA"
                     /locus_tag="RH222_002111"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV3797128.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEEVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREGICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21933..22826)
                     /gene="argP"
                     /locus_tag="RH222_002112"
     CDS             complement(21933..22826)
                     /gene="argP"
                     /locus_tag="RH222_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV3797129.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22968..23198
                     /locus_tag="RH222_002113"
     CDS             22968..23198
                     /locus_tag="RH222_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797130.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            23254..23913
                     /gene="rpiA"
                     /locus_tag="RH222_002114"
     CDS             23254..23913
                     /gene="rpiA"
                     /locus_tag="RH222_002114"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV3797131.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24169..25401
                     /gene="serA"
                     /locus_tag="RH222_002115"
     CDS             24169..25401
                     /gene="serA"
                     /locus_tag="RH222_002115"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV3797132.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25630..25689
                     /gene="ibsC"
                     /locus_tag="RH222_002116"
     CDS             25630..25689
                     /gene="ibsC"
                     /locus_tag="RH222_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV3797133.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            26022..26081
                     /locus_tag="RH222_002117"
     CDS             26022..26081
                     /locus_tag="RH222_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3797134.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26182..26784)
                     /locus_tag="RH222_002118"
     CDS             complement(26182..26784)
                     /locus_tag="RH222_002118"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV3797135.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26805..26988)
                     /gene="ssrS"
                     /locus_tag="RH222_002119"
     ncRNA           complement(26805..26988)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RH222_002119"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27030..27359)
                     /gene="zapA"
                     /locus_tag="RH222_002120"
     CDS             complement(27030..27359)
                     /gene="zapA"
                     /locus_tag="RH222_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV3797136.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27527..28105
                     /gene="ygfB"
                     /locus_tag="RH222_002121"
     CDS             27527..28105
                     /gene="ygfB"
                     /locus_tag="RH222_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV3797137.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28131..29456
                     /gene="pepP"
                     /locus_tag="RH222_002122"
     CDS             28131..29456
                     /gene="pepP"
                     /locus_tag="RH222_002122"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV3797138.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29453..30631
                     /gene="ubiH"
                     /locus_tag="RH222_002123"
                     /gene_synonym="visB"
     CDS             29453..30631
                     /gene="ubiH"
                     /locus_tag="RH222_002123"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV3797139.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAVL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30654..31856
                     /gene="ubiI"
                     /locus_tag="RH222_002124"
                     /gene_synonym="visC"
     CDS             30654..31856
                     /gene="ubiI"
                     /locus_tag="RH222_002124"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV3797140.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDTTLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAAMMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31930..>32010)
                     /locus_tag="RH222_002125"
     CDS             complement(31930..>32010)
                     /locus_tag="RH222_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797141.1"
                     /translation="SKNTKSKIRDIRKYNAKTEVKLVYFK"
BASE COUNT         7654 a         7929 c         8626 g         7801 t
ORIGIN      
        1 taattcactg ttttcaaaac atcggtttcc ctgctcaatt gctttcatta aaccgcacga
       61 gtattttatt tttaccgccg gacggtaccc taaaaataaa ataaggataa tttatgcggc
      121 ttagtgatta ttttccagaa tcatcaatct cagttataca ttcagcaaaa gattggcagg
      181 aagctatcga tttctcgatg gtatcattgc tggataaaaa ctatatcagc gagaattaca
      241 ttcaggcaat taaagattcc accattaaca atggccctta ttatattctc gcaccaggcg
      301 tggcaatgcc tcatgcgcga ccggaatgtg gggcgcttaa aaccgggatg tctttgacat
      361 tacttgaaca aggtgtttat ttttcgggga atgacgaacc aattaaatta ctcatcggac
      421 tctctgctgc cgatgccgat tcgcacattg gcgctattca ggcgttaagt gaattactgt
      481 gcgaagaaga aatactcgaa caactcttaa cagcatcatc agaaaaacaa ttagcggaca
      541 ttatcagccg cggataatta ctctctcctc tacaggataa tattatggaa aacaagtctg
      601 ctcgtgcaaa ggtccaggct tttgggggct ttttgactgc aatggtcatc cccaatattg
      661 gtgcttttat tgcctggggc ttcataactg cgttatttat tcccaccggt tggctgccga
      721 atgaacattt cgccaaaatt gtcggcccga tgattaccta tttattgccc gtgatgattg
      781 gttctactgg tggtcatctg gtcggcggta aacgcggggc ggtcatgggc ggaataggta
      841 ctattggtgt gatcgttggc gcagagatcc cgatgttcct tggctcaatg atcatggggc
      901 cgctcggtgg gttggtcata aaatatgtcg ataaggcact ggaaaaacgc atacctgccg
      961 gttttgagat ggttatcaat aacttctcat taggtatcgc ggggatgctc ctttgtctgc
     1021 tgggttttga agtgatcggc ccggcggtgt taattgccaa tactttcgtc aaagagtgta
     1081 tcgaggcgct ggtacatgcg ggttatctgc ctctgctgtc agtcatcaat gaaccggcga
     1141 aagtgctttt ccttaataat gcgatcgatc agggcgtcta ttatccgctg ggaatgcaac
     1201 aggcttcggt taacggtaaa tccatcttct ttatggtggc ctctaaccca ggtccgggcc
     1261 tggggctgct gctggcgttt accttgtttg gtaaagggat gagtaaacgt tctgcgcccg
     1321 gggcgatgat tattcacttc ctcggtggga tccacgaact gtatttccca tatgtgctga
     1381 tgaagccgct gactattatt gccatgattg cgggcggtat gtctggtacc tggatgttta
     1441 acttactgga cggtggtctg gtggctggcc caagtccggg gtctatcttt gcttacctgg
     1501 cactgacgcc gaaaggctcg ttcctggcga caattgccgg tgttacggta ggtaccctgg
     1561 tgtcctttgc tattacttcg ctgatactga agatggaaaa aacggtggaa acggagagcg
     1621 aagatgagtt tgctcagtca gccaatgcgg ttaaggcgat gaaacaagag ggtgcattct
     1681 cgttaagcag ggttaagcgt atcgcctttg tttgcgatgc ggggatgggc tccagtgcga
     1741 tgggcgcgac caccttccgt aaacgcctgg aaaaagcggg gctggcaata gaagtaaaac
     1801 attacgcgat agaaaacgtg cctgcggatg ccgatatcgt cgttactcat gccagtctgg
     1861 aagggcgcgt gaaacgtgtt acggataaac cgctgatatt gattaataac tatattggcg
     1921 atccaaaact cgacacttta tttaatcaat taaccgccga acataaacac tgattacaga
     1981 ggtaaaaatg aaaaccaaag ttgctgctat ttatggcaag cgggatgtcc gtctgcgcga
     2041 atttgaactg ccagaaatta ccgataatga actgttagtg agtgtaattt ctgacagcgt
     2101 ctgtttatcg acctggaaag cggcgttact cggtagtgaa cataaacgcg tacccgacga
     2161 tttagaaaat catccggtca ttaccgggca tgaatgtgcc ggggttattg tcgaagtggg
     2221 taaaaatctc actggcaaat ataaaaaagg ccagcgtttt gtattgcaac cggcgatggg
     2281 gttaccaagc ggatattcag caggctacag ctacgaatat tttggcggta acgccaccta
     2341 tatgattatt ccctaaatcg ccattaattt gggctgcgta ttaccgtatc acggctctta
     2401 ttttgctgcg gcgtcgctgg cagagcctat gtgctgcatt attggtgctt atcatgccaa
     2461 ttatcacacc acgcaatatg tttatgagca tcgcatgggc gtcaaacctg gcggcaatat
     2521 tgcactgctg gcgtgtgcgg gtccgatggg cattggcgct atcgattacg ccattaacgg
     2581 tggcatacaa ccgtcacggg tggtggtggt cgatatcgac gacaaacgcc tggcgcaggt
     2641 gcagaagctg ctgccggtgg atctggcggc cagtaaaggc attgagctgg tgtatgtgaa
     2701 taccaaaggg atgagcgatc cggtccagac gctgcgggcg ctgacaggag atgtcgggtt
     2761 cgatgacatt tttgtttatg cggcggtgcc tgctgtcgtt gagatggctg acgaattact
     2821 ggcggaagat ggctgtctga acttctttgc cgggccgacg gataaaaact tcaaagtgcc
     2881 gtttaatttc tacaacgtcc attacaacag cacgcacgta gtcggtacat ccggcggttc
     2941 aacggacgac atgaaagagg cgattgcgtt aagcgccacg gggcagttgc agccgtcctt
     3001 tatggtcacg catatcggtg ggctggatgc ggtgccagat accgtgctca atctgccgga
     3061 tatccctggc ggtaaaaaac tcatttataa cggcgtgacc atgccgctca ctgccattgc
     3121 cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa gagttggcgc ggctggttga
     3181 ggaaacgcac ggcatctgga atgaacaggc cgagaaatat ctgctggcac aatttggcgt
     3241 tgatatcggg gaggccgcgc aatgatgtcc ctggcgtggc cattatttcg cgttacggaa
     3301 caggcagcgt tggctgcctg gccgcaaacc ggatgtggcg acaaaaataa aattgatggc
     3361 ctggcggtca ccgcgatgcg ccaggcatta aacgacgtcg cttttcgtgg acgagtggtt
     3421 atcggcgaag gagaaattga ccatgcgccg atgctgtgga ttggcgaaga ggtgggcaaa
     3481 ggcgatgggc cagaagtcga tatcgcggtt gacccgattg aaggcacgcg gatggtagcg
     3541 atggggcaga gcaatgcgct ggcggtcatg gctttcgccc ctcgagatag cctgttacat
     3601 gcgcccgata tgtatatgaa aaagctggtt gttaatcgac tggctgccgg ggcgattgat
     3661 ctgtctttgc ctctggcgga caacttgcgt aacgtggcgc gggcgttagg taaaccgctg
     3721 gacaagctgc gcatggttac gctggataaa ccgcgcctga gtgccgcgat tgaagaagca
     3781 acacagctgg gcgtgaaggt tttcgctctg ccggatggcg atgtcgccgc cagcgtgctg
     3841 acctgctggc aggataatcc ttacgacgtg atgtacacca tcggcggtgc gccagaaggc
     3901 gtgatttctg cctgtgccgt taaagcatta ggtggggata tgcaggccga gctgattgac
     3961 ttttgtcagg ctaaagggga ttacacggaa aatcggcaga ttgcagagca ggagcgcaag
     4021 cgttgtaagg caatgggcgt tgacgttaac cgtgtgtact cgctcgatga actggtgagg
     4081 gggaacgata tcctctttag cgctacgggc gtgacgggcg gcgagttggt gaacggtatc
     4141 caacagacgg cgaatggggt gcggacgcag acattactga tcggcggcgc ggaccaaacg
     4201 tgtaatataa tagactccct gcattgatgg tgatctatcg gaagaccaat ggcgacgctg
     4261 acagaagatg atgtgcttga gcaactggat gcacaggaca atttattttc atttatgaaa
     4321 actgcgcatt ctattttgct ccaggggata cgccagtttc tgccgtcgct gtttgtcgat
     4381 aacgatgaag agatcgtcga atatgcagtg aagccgttac tcgcccaaag cggcccgctt
     4441 gacgatattg atgttgcgct gcgtttgatt tatgcgctgg ggaaaatgga taaatggctg
     4501 tatgccgata tcacgcactt ttcccagtac tggcattacc tgaacgaaca ggatgaaacg
     4561 cccggatttg ccgatgacat gacctgggat tttatcagca acgtcaatag cataacctgc
     4621 aatgcgacgc tctatgacgc gttaaaagcg atgaagttcg ccgatttcgc cgtctggtca
     4681 gaggcgcgtt ttagcggaat ggtcaaaacg gcgctgacgc tggcagtaac gaccacctta
     4741 aaggaattaa cgccgtgaaa attgaattaa cggtgaatgg gctgaacgtg caagcgcagt
     4801 accatgatga agagatagaa cgcgtgcata aaccgttgct gcgtatgctg gcggccttac
     4861 agacggtaag tccgcagcga cgtacggtgg tttttctttg cgctccgccg gggacgggta
     4921 aatctacctt aactaccttc tgggaatatc tcgcgcaaca agaccctgaa ttacccgcta
     4981 tccagacgct cccgatggat ggttttcacc attacaatag ctggctggat gcgcatcaat
     5041 tgcgcccctt caaaggcgca ccagagacat ttgacgttgc gaaactggcg gaaaatctgt
     5101 gccaggtagt ggaaggggat tgtacgtggc cgcagtatga tcgacaaaag catgatcctg
     5161 ttgaagatgc gttgcacgtt accgcaccac tcgtcatcgt cgaaggaaac tggttgttgc
     5221 tggacgacga gaagtggtgc cagcttgcgc aattttgtga tttctcgatc tttatcaacg
     5281 cgccagctac tgcgttgcgg gaacgactgg tagggcgcaa gctggcggga ggggtatcgc
     5341 tggcagacgc agaagctttt tatgaccgta ccgacgggcc gaatgttcgc cgggtgctgg
     5401 aagagagtct gcctgcaaat ttgaccttaa tgatgaccgc tacgggtgaa tatcgtctgg
     5461 tggattaacg ttcctgatta gaccatcttc cagtcgcacc acacgagaga aatgacgccg
     5521 ggtaaatgcg gcgtcatgac tgattgctac gacgctcgtg cctcgctggc cgcacttctc
     5581 cagccagctt tcaaacacgc tcaaccaatt ttcgtcaaaa tcccgactcg gctcgtccag
     5641 cagcaatagt ggcggagaga gtgcttccag gcaggcgacg gcgaccatcc gtcgctgcgc
     5701 actatgcaga tccaacgggt gcgagttggc agtgtctgct aactgacaac attgcagggc
     5761 agcatgggta cgttgagtga tttcatccgc cgggcatttt tgcagtttca aaccaaatgc
     5821 gatctcgtca gcgacggtgc tgtgaaaaag ttggttttcc gcttcctgaa acagtacgcc
     5881 aacctttgcc gcgcgctggc ggtttttcag gtttttcatc gcctgttgtt gcagcataac
     5941 cgtaccggct gtgggcgtaa gaagacctgc cattactcgt aacagcgtcg attttcccgc
     6001 gccattgtcg ccggtcagcg ccaaccattc accttgtttg agttgcagag aaatgtcgca
     6061 aagacaatcc gttgccgcgc caggccagcg ataagatatc ttatttaacg ttaacatagt
     6121 ggctggagcg ctccgttttg caattgccac gcttgctggc agtagttcag gaaaggtgtg
     6181 cgatgacgtt caaaaagaat aatcagcgaa ccacgttcaa acgcccagtg ttgtaagcgc
     6241 tgcaacagca tttcgctggc ctgcggagtc aggcggctaa aggcttcatc aagaattaag
     6301 agctttggct gcatcgcgat ggcgcaggcg atgaccacgc gctgtgtctc tccaccggag
     6361 agtgttgccg ggtgacgatg gcgcaacggc tggcactcgg tcagagtcag ggcggcatca
     6421 atccgcgcca taatctctgc ttcagcaaga cacagatttt ctggcccaaa ggcaacttct
     6481 tcttccacgc taaaggtaca gccggagagc tgcaaatagg gcgattgctg gacgagctgg
     6541 atggtggcgg attgttcatt aagcgggaga tgcccaatgg gcgtaccgag gagcgtcccc
     6601 gtgcctgtta tctctcctgg cagaaagtcc ggataccagc ccgccatcag ctgtgccagc
     6661 gtacttttgc cactgccgtt gtcaccaaag atggcgacca tccccggttt gacatagtga
     6721 aagtcataac agaacggagg atgcgttgag tgtgtcgggc aataacggaa ctgttctaac
     6781 gtaaccatat ccacgctccg aattctgtca gcatcagcaa gatcatggtg taacgcgcta
     6841 cgcgttgtag ggtgctatcg gctggtgccc acaacgttgt tcgtcggttg tggatccgaa
     6901 atgcccgcat atccagcgcc gcaccacgga cagccagatc gttaagcgca ttgtgtgtta
     6961 gcggaataat taacgcgggc atcgcacgta aacgctgata ccagccctca tccagcggca
     7021 cgccgcgggc acgctgcgct tcatgaataa tcgctaattg ccgttttaac tgctcaacga
     7081 ccaataacgg cccggcaaac agataagcga cgcctggcgg caggcgagaa gcaaacagag
     7141 cgcgaataaa acattgcacg gggacgtact gcatccacaa ctgtgaggtt gaaacaatcg
     7201 ccagaatccg caaccagagc gtaatagcat gcgaccaccg ttctggcgaa cgcggcgtgc
     7261 cgcttagcca ttctgttagc cagccaccgt gtaccagcca cagcccggct cccagcgaga
     7321 acatcagcca gacgacatat tttgcccgcc gccgcgtggc ctttagcgca ataaggcaaa
     7381 aaaaggtcgc cgcgctatag atcggcaaaa tggtctgcgc gggcaggata agcgtggtgc
     7441 aggccgccaa tgcccatagt gttaatgagg taaatggatg catttagcgc acggcagcca
     7501 ttgccggaaa atggcgcgtg gtgcgaatcg gcagctggcg cagtaaaagc cagacaatca
     7561 ccgctgtaag aattttatcg accaggttcg cgcctatcac cgtaatcgcc accgactcca
     7621 ccaggttttg ccccatagag tgcatccagg caacgaacaa atcggctccg ctaccggtga
     7681 caccgccgaa cagcgcagta cgcagcggta cagcgacgac cgttaccgca agcgtaataa
     7741 tcacgccgct gacaacgact tttggcaggg tgcgaaacca acccgctcgc gccagccaac
     7801 cggcgaccag gccgatgacc atcgcgacgg gggcaaatgc ggcggcgatc gggtcggtca
     7861 gcaatcccca gagtaaattg gtcagtaagc cggttagcat cccgatcacc ggaccaagca
     7921 gtaccgcgct aataagggtg ccgatggagt cgagaaatat aggtaatttg accatgctgg
     7981 ccagttgccc gccgatcata ttgatggcga tagatataac gatcaacacc agtgcctgac
     8041 tggaaaagtg gctacgcgcc ataagaaaac ctctctgaaa gtagttattt aacgggggaa
     8101 atatgcaact cgtcgtaacc agaaagcgcg caaggttggc ggctaaacgc cagttcctca
     8161 aggctaccga tcactagttc aagcgtggtc cgcacggtac agccgggcat catatgaccg
     8221 ccaagcatcg tgccgtgtgg atcggaaacg cagagatgaa gatgctcacc actctgttcc
     8281 agcgtgccgt ttagtgagat cacttcgaat gtaccgttca ggagcgtggt gccttcttgc
     8341 ccggcatagc gcaaggcgac gtcagtcagg ctaccggtac agcctgcaat ccaggcggca
     8401 tgaagttgct gctgctgtgc aaaagcgtgt agctgggaaa aaacttcctg cccggggagc
     8461 aggcgcagtg cgtaaaagcg tgcggaggaa gaatatgggt agggcaatgt catcattgga
     8521 gtactccgaa actcggacgt attgcggata atatcgacgc tgcatcagtc atattctgat
     8581 ataagtcagg gtttgtgacg gcacaacgaa aagattttgc tacgcttatt cgtgattctg
     8641 caaagtggaa taaattccac aggggcgggg ctgaaaacgt gacgtaagtc agatatttgt
     8701 tttctttgac tggacaatca ttccttttat tccacgtttc gcttatccta gctgaagcgt
     8761 ttcagtcgat taaatgttcg acaattaacc aatcagtcgc agtttgcgac aggtaaggtt
     8821 tccccggacg atttgctgga ttactctgtc tggcaaattt gctcagggaa aaccttgcag
     8881 gagatctatg accgtacgcg tagcgataaa tggcttcggt cgcatcgggc gtaatgtggt
     8941 tcgtgctttg tatgaatcag gacgccgtgc ggaaattacc gtggtggcaa tcaacgaact
     9001 ggcggatgct gcgggcatgg cgcatttgtt gaaatatgac accagtcatg gccgttttgc
     9061 atgggaagta cgacaggaac gcgatcaact ttttgttggt gatgacgcca tccgcgtatt
     9121 gcatgaacgt tcactgcaat cgctcccctg gcgtgaactt ggcgttgatg tagtcctcga
     9181 ctgcaccggc gtatatggct cccgcgagca tggcgaagcg catattgccg ccggggccaa
     9241 aaaagtgctc ttttcacatc ctggcagtaa cgatctcgac gcgaccgttg tttacggcgt
     9301 caatcaggat caacttcgtg cggaacaccg catcgtttct aacgcttcct gtaccacgaa
     9361 ttgcataatt cccgtcatca aattgttaga tgatgcgtac ggtattgagt ccggcactgt
     9421 gaccacaatt cactccgcca tgcacgatca acaggttatt gatgcatacc atcctgacct
     9481 gcgtcgcacc cgggcagcca gccagtcgat cattccggtc gatactaaac tggccgccgg
     9541 tatcacacga ttttttccgc aatttaacga tcgctttgaa gcgattgcgg tacgtgtgcc
     9601 aaccataaat gtgacggcaa tcgatttaag cgtgacggtg aagaaacctg taaaagccaa
     9661 tgaagtcaac ctgttgctgc aaaaagcagc acaaggtgca tttcatggta tagttgacta
     9721 tacggaattg ccgttggtct ctgtagattt taaccacgat ccgcacagtg ccattgtcga
     9781 tggcacccaa acccgggtca gtggcgcaca cctgatcaaa acgttggtct ggtgcgataa
     9841 cgaatggggc tttgctaacc gaatgctcga cacgacgtta gctatggcta ctgttgcttt
     9901 caggtaagac gcaagcagcg tctgcaaaac ttttagaatc aacgagagga ttcaccatgt
     9961 ctgtaattaa gatgaccgat ctggatcttg ctgggaaacg tgtatttatc cgtgcggatc
    10021 tgaacgtacc agtaaaagac gggaaagtaa ccagcgacgc gcgtatccgt gcttctctgc
    10081 cgaccatcga actggccctg aaacaaggcg caaaagtgat ggtaacttcc cacctgggtc
    10141 gtcctaccga aggcgagtac aacgaagaat tctctctgct gccggttgtt aactacctga
    10201 aagacaaact gtctaacccg gttcgcctgg ttaaagatta cctcgacggc gttgacgttg
    10261 ctgaaggtga actggttgtt ctggaaaacg ttcgcttcaa caaaggcgag aagaaagacg
    10321 acgaaaccct gtccaaaaaa tacgctgcac tgtgtgacgt gttcgtaatg gacgcattcg
    10381 gtactgctca ccgcgcgcag gcttctactc acggtatcgg taaattcgct gacgttgcgt
    10441 gcgcaggccc gctgctggca gctgaactgg acgcgctggg taaagcactg aaagaacctg
    10501 ctcgcccgat ggtggctatc gttggtggtt ctaaagtatc taccaaactg accgttctgg
    10561 actccttgtc taaaatcgct gaccagctga ttgttggtgg tggtatcgct aacaccttta
    10621 tcgcggcaca aggccacgat gtgggtaaat ccctgtacga agctgacctg gttgacgaag
    10681 ctaaacgtct gctgaccacc tgcaacatcc cggttccgtc tgatgttcgc gtagcaaccg
    10741 agttctctga aactgcaccg gctaccctga aatctgttaa cgatgtgaaa gctgacgagc
    10801 agatcctgga tatcggtgat gcttccgctc aggaactggc tgaaatcctg aagaatgcga
    10861 aaaccattct gtggaacggt ccggttggcg tgttcgaatt cccgaacttc cgcaaaggta
    10921 ctgaaatcgt ggctaacgct atcgcagaca gcgaagcgtt ctccatcgct ggcggcggcg
    10981 acactctggc agcaattgac ctgttcggca ttgctgacaa aatctcctac atctccactg
    11041 gcggcggcgc attcctcgaa ttcgtggaag gtaaagtact gcctgcagta gcgatgctcg
    11101 aagagcgcgc taagaagtaa aaaatcacag ggcagggaaa cctgcccttg tttcagcgcg
    11161 cttttagagc acgcattttt tcatactcta aataattcga gttgcaggaa ggcgacaaga
    11221 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
    11281 acatgcaact tgaagtatga cgagtataag gcccgacgat acaggacaag agacatgtct
    11341 aagatttttg atttcgtaaa acctggcgta atcactggtg atgacgtaca gaaagttttc
    11401 caggtagcaa aagaaaacaa cttcgcactg ccagcagtaa actgcgtcgg tactgactcc
    11461 atcaacgccg tactggaaac cgctgctaaa gttaaagcgc cggttatcgt tcagttctcc
    11521 aacggtggtg cttcttttat cgctggtaaa ggcgtgaaat ctgacgttcc gcagggcgct
    11581 gctatcctgg gcgcgatctc tggtgcgcat cacgttcacc agatggctga acattatggt
    11641 gttccggtta tcctgcacac tgaccactgc gcgaagaaac tgctgccgtg gatcgacggt
    11701 ctgctggacg cgggtgaaaa acacttcgct gctaccggta agccgctgtt ctcttctcac
    11761 atgatcgacc tgtctgaaga atctctgcaa gagaacatcg aaatctgctc taaatacctg
    11821 gagcgtatgt ccaaaatcgg catgactctg gaaatcgaac tgggttgcac cggtggtgaa
    11881 gaagacggcg tggacaacag ccacatggac gcttctgcac tgtacaccca gccggaagac
    11941 gttgattacg catacaccga gctgagcaaa atcagcccgc gcttcaccat cgcagcgtcc
    12001 ttcggtaacg tacacggtgt ttacaagccg ggtaacgtgg ttctgactcc gaccatcctg
    12061 cgtgattctc aggaatatgt ttctaagaaa cacaacctgc cgcacaacag cctgaacttc
    12121 gtattccacg gtggctccgg ttctactgct caggaaatca aagattccgt aagctacggc
    12181 gtagtgaaaa tgaacatcga taccgatacc caatgggcaa cctgggaagg cgttctgaac
    12241 tactacaaag cgaacgaagc ttatctgcag gggcagctgg gtaacccgaa aggcgaagat
    12301 cagccgaaca agaaatacta cgatccgcgc gtatggctgc gtgccggtca gacttcgatg
    12361 attgctcgtc tggaaaaagc attccaggaa ctgaacgcga tcgacgttct gtaagatatt
    12421 cctttctgct tatctcaagg cccgctctgc gggtcttttt ttcgccaaaa gcaaaattgc
    12481 ctgatgcgct acgcttatca ggcctacacg tatcctgcaa tatattgaat ttgcaggttt
    12541 ttgtaggccg gataaggcgt tcacgccgca tccggcatga caaagcgcac attgatatct
    12601 gtctgagccc cgctctgcgg ggattttttc gccaaaagca aggtgattca gatgagaaat
    12661 ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact gcccgtcata
    12721 aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg aatggaagat
    12781 ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa ccaggcgctg
    12841 ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt tggtttgatt
    12901 atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa aatcgatgcc
    12961 actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt tacgctaatc
    13021 gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact cggtgccgca
    13081 ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc tggcgtgtta
    13141 cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg cgtagccggt
    13201 actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg taaaattatc
    13261 gttattccga acggtaaaat tattgccgga aatattatta acttctcccg cgagccagtt
    13321 cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca ggttaagcag
    13381 atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga aatgactgtg
    13441 cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg gagcaacagc
    13501 ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga atttgatgcc
    13561 gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt gaaagaagac
    13621 aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc ttattagtta
    13681 ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga tatagtctct
    13741 gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca aggtcttgct
    13801 cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat gaatcagggc
    13861 attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga tttggtcctg
    13921 atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg gttgctggcg
    13981 ctggtcacct ggggcggcgt agtcttcttg ctgtggtatg gttttggcgc ttttaaaaca
    14041 gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg cagatggaaa
    14101 attatcgcca ccatgttggc agtgacttgg ctgaatccgc atgtttacct agatactttt
    14161 gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg gtttgcgctc
    14221 gggacaatta gcgcttcttt cctgtggttc tttggtctgg ctattctcgc agcctggctg
    14281 gcaccacgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt gggatgtgtc
    14341 atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca agccttgttc
    14401 agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct ccagaggtcc
    14461 tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa gttatcgccc
    14521 tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg ccggatggac
    14581 cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt gccactcttg
    14641 cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag gcagatgagc
    14701 gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa gatatcagct
    14761 cagcgaattt acgcacccag ccagattatg attatcagga tggtaaaagt atcctgaaag
    14821 gctaccgcgc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa ctgaattcct
    14881 tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttcagtgtcg ctgggcgtgg
    14941 cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac gcgattcatc
    15001 aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat agcgtgcgct
    15061 accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc gatgccgcgc
    15121 cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat caggtcgatg
    15181 tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca gcacaataat
    15241 acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc tcctttttct
    15301 gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt catcaaggat
    15361 aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga catttttatt
    15421 tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc ccgggcggca
    15481 gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct ggaagaacaa
    15541 attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa agccggtcag
    15601 gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga taatgaaatc
    15661 cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac ttatccgaca
    15721 attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac cgcccagcca
    15781 gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt agattttatt
    15841 atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac tatcagtgaa
    15901 ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagggcg tcaagagcaa
    15961 gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc taacttcgaa
    16021 gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat ttatcgtccc
    16081 tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac ggtgatcccg
    16141 cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc tccgcacaag
    16201 ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac cgccacgatg
    16261 cttcagcata ttgctgaaga tcgtgacggg acgagtcatt aacccagcat cgagccggtt
    16321 gcaattaaat tacggtgtaa gtcgaagacg tggctaagat cgtggtgaat atgtccgcca
    16381 ggcgcatttt ccagatagcg atgcagataa tcctgataca taggatgtgc acaattatca
    16441 atgatagtgc gggcgcgttg aagcggggaa agaccgcgca gatcggcgat cccttgttcg
    16501 gtgatgatca ctttgacgct gtgttcgctg tgatcaacat ggctgcacat tggcacgacg
    16561 gttgagatct tgccttcttt agcaatcgac ggggccataa agatcgacag atacgcgttg
    16621 cgttcaaaat caccgctgcc accgatgccg ttcatcagat cgaccccggc tacgtgcgtt
    16681 gagttggcat gcccgtaaat atcaaactcc aggccgacgt tcagagcgat gacgcccaga
    16741 cgacggatga tttccgggtt attggaaatc tcctgcggac gcaacacaat gcggctggca
    16801 aagtaatcca tattgtcgta aatcttgcgc agggaatcgg ccgagattgt caggctggag
    16861 gcgctggccc cgctgatttt gccggtttcc agtaaatgca ccaccgattc ctgtagcact
    16921 tccgaataca tcataaacgg aggaatttcc gggttttccc ccagacgcgc cattaccgca
    16981 ttattgatat tgcccacgcc actttgcagc ggcagaaatt ccggcggaat acgcccatgc
    17041 gccatttcct gcaacaagaa cgtgaccacg ttatcggcaa tctgctggca catgggattt
    17101 tgcttatcca gcatattacc ggcgtcgggc aagttggttt ccacgacggc gacaatcttt
    17161 ttcggatcga tttgcacata gcgggtaccg acgcgatcca ttgcatggaa gatcgacacg
    17221 ctattgcgcc gcggtggcgc gccaggaatc acaatatccg ccagttctgc aacgcgcgga
    17281 tcgtgatagt gattgagttc aatgatcact ttcttcgccc gcagcagcca ggtcggcgca
    17341 ttaccgatcc cgctggttaa ccagactcga ccatccggtg ccagtgccga tgcttcaatg
    17401 acggcaacat caatgtcgcc gaagaaaccg taattgacca tttgcgccac ttcgctcaaa
    17461 tgcaggtcaa cgaaactcac cgcgccctga ttgatctttt tacgtaaacc ggacgatgtt
    17521 tgatatggcg cacgccagga aacagcatcg gcgtcagaaa gtacatcgtc agcggcggcg
    17581 ctgattgacg cacccgtcag aaggcgaatt tgatacggct ttttggcctc atgctgttcg
    17641 ttagctctgc gggcaatcgc ggtgggtagg gctttcggcg aacccgccgg ggtaaagccg
    17701 ctaaatgcca ccatgtcgtt atgctggata atttctgccg cttcattggc ggtcatcctt
    17761 gtccactgag tttccatttt tactccttcg ttcgcaggga ttaatgacca acgaaattag
    17821 gtttacgttt ttcgaggaaa gcgttcatcc cttcctggta atcttcgctg tcatacaccg
    17881 cgcggcgcat cccctgaata cgttcaaatt catcggagtt catggtgtgt gcttcgccca
    17941 gtacacgcag ctcttctttg ataacggcaa tggctaacgg cgctttctca gagatgtggt
    18001 gcgccatttg taaggtgaaa tcttccagtt cttccacttc cacaacatgg ttgaggatgc
    18061 cgacagccag cgcgcgctgg gcggtgattg gcgaagcggt aaaaatcagc tctttgacaa
    18121 tgtggaagcc cgcgtcgcgg gtcaggttgt gaatgccgac caggttatac gggacgccga
    18181 ggtttacagg cgtcattgag aaggttgagg tactggcggc gatgatcaga tcggaactca
    18241 tgatcatttc aaatgcgcca ccccaaacac taccttccac catcgaaatg atcggtttcg
    18301 ggaatttttg gatcatgcgg gtgatttgac gcaatggatc atcataggag agcggatcgc
    18361 gaccgccaga cggcagttcg tgaatatcgt ggcctgcgga gaagactttg gatccactcg
    18421 gcgcgcgcaa aatgatacag cgaatttccg gccggttgag atcgcttaat gcctgcataa
    18481 gatcatcaat aaagacttta cttaaggcat taagtttgcg gccatagtta aactcaatga
    18541 ccgccacttt gttgatagtg acaacgttaa catactgata agacataaaa attcctttaa
    18601 tcaaaatatt gcgtctggat aaattcactg agctgccgca ggccggtgcg cggtgagagc
    18661 gtattgtttt tgaccgctaa aagcgtctgg cggtaatagc gatcgaaatc ttcattcgcg
    18721 aacaggtgat tcagtacttc ttcttcggtc tgcttacgca gccattccac cgattgttgt
    18781 tgccgcactt gttgtaaacg accactggca gttagcgcgg ttttgaagtt gatgatggcg
    18841 tgccagatct catcgattcc acgtttttcc agtgcgctac aagtcagaac ccgtggctgc
    18901 cattcgtcgt atttacgtcg cagaatatgc agggcactct cgtacatatg ccgggcaatg
    18961 gcgacattgg tatggttatc gccatcgtct ttgttgataa cgagcagatc agccacttcc
    19021 atcagccctt ttttaatgcc ctgcagatca tcgccgccac cggcaatttg caacgagata
    19081 aaacagtcca ccatgcgggc gacttccgtt tccgactgcc cgacgccaac cgtttcgaca
    19141 atcactacgt cataacccgc cgcttcgcat aacagcatta attcccgcgc tcgctgactg
    19201 gcaccgccca gatgaccgga ggatggtacc gggcgaataa acgccgcttc ggcacgcgcc
    19261 aggtcattca tgcgggtttt atccccgaga atgctaccgc cagtgaccgg gctgctggga
    19321 tcgaccgcaa taaccgcgac ctttaatccc tctcgaatca acaacatgcc aaaggcctca
    19381 agaaaggtac ttttccccgc gccgggggtg ccggtaacgc ccagtcgcag ggtgttaccg
    19441 cagtacggca taatggtatc aagcagctgc gtacttagtg cctgatgacg cgggtgacgg
    19501 ctttccacca gcgtcatggc ctgggcgagt gtggcacgct caccctgacg taagcggcga
    19561 atactttctg ccagcgtggc ttcattaatc atgatgctgg cttatcagat tcagtacgtc
    19621 gcgcacactg tcgagcatag gtgtacctgg accataaatc gccgccacgc cgcgctcttg
    19681 caggaaggcg taatcctgcg gcggaatgac gccacccgcg accacgcaga taccttcgcg
    19741 tccccatttt ttcagcgctt cgaccagttc cgggatcagc gttttatgac cggcagccag
    19801 tgaggatgcg cccactacgt gaacgtcgtt ttctacggcc aggcgggcga tctcttcagg
    19861 tgtagagaac atcgggctta aatctacgtc gaaaccgaga tcggaatagg cgctggcgat
    19921 cactttcgcg ccgcgatcgt gtccatcctg gcccatctta gcgatcagaa tgcgcgggcg
    19981 acgaccattg tcggcaagga actgctccgt ttgcgcaaca atggcatcga actcggaggc
    20041 cgatttctcc gactgatgat agctttgcgc aattacgccg gtaacacact ggcttggcac
    20101 cagataacgg tcgaaagcgg cttccagcgc atcggaaatt tcacccaggg tggcgcgaac
    20161 gcgagcggca ttaacagcgg cagccagcag gttttcgtta tgctgtgcgg cgtgagtcag
    20221 ggcgttcaac gcggcggtta cggcggcatc atcacgggtg gcgcgaatgc gttccagcga
    20281 agcaatttgc tcgttacgca ccatcacgtt gtcgatctca agtacatcgg tttcgtcttc
    20341 gtgatccagt ttgtacttgt tgacaccaac gatgacacgc ttgccctggt cgatcagcga
    20401 ctgttcgcgc gctgaggcct cttcgatcat tcgttttggc agacctgctt cgatcgcttt
    20461 cgccatgcca ccggcttcgt cgatctgttg gataatagct ctggcttgtt tgacgatttg
    20521 atcggtcagc gactcaatgt aataggatcc ggccagtgga tcgacggtgc ggcagagttc
    20581 tgattcttcc tggatgatga tctgggtgtt gcgggcaatg cgtgctgaga aatcggtagg
    20641 caaaccaagc gcttcgtcaa aggcgttggt atgcagtgac tgagtaccgc ccagcgtcgc
    20701 agccagcgct tcaatggtgg tgcggataac gttgttatac ggatcctgtt cagtcaggct
    20761 ccagcctgag gtctggcagt gggtacgcag cgccagtgat ttcgggtcct gtgcgccaaa
    20821 tccactgacc tcttcgctcc ataaataacg tgccgcacgc aacatggcga cgttcataaa
    20881 cagatccatg ccgatgccga agaagaacga caggcgagga gcgaagtcat caattttcag
    20941 tccggcagag attgctgctt tgatgtactc aatcccatca gcgagcgtaa atgctacctg
    21001 ctgcacgcag ttggcacccg cttcacccat gtggtaaccg ctgatactga tggtattaaa
    21061 tcgcggcatg ttgccggaac accaggcgat gatgtcggcg ataatgcgca ttgacggttt
    21121 tggcgggtaa atataggtgt tgcggcagag gtactctttg agaatatcgt tttgaatggt
    21181 gccggtcagt ttatcaggtg taacaccttg ctcttctgcg gcgacgatat aaaacgccag
    21241 tactggtagc actgcgccat tcatggtcat cgaaaccgac attttatcca gcgggatctg
    21301 gtcgaacagg actttcatat cttccacggt gtcgatagcg acgcccgctt tgccgacgtc
    21361 acccgccacg cgtgggttat cggagtcgta gccacggtgg gtggcaaggt caaacgcaac
    21421 ggaaagacct ttttgcccgg cggccaggtt acggcgataa aaagcgttgg actcttttgc
    21481 tgttgaaaaa ccagcatact gacggatggt ccacggttgg gcggtataca tagtggcacg
    21541 cgggccacga acgtagggcg gcaaaccagg aagggtacct gtcacctcca gattatcgag
    21601 atcggcttcg gtatacagcg gcttgatggc gatcccttcc gcggtttgct gaaccagcga
    21661 gtcgacagtt ttctcccgac ggctcaattc cttgttggca agctgttgcc actcctgctc
    21721 gttagacatt atttgctccg taaaacgcaa tcaaaatgag ggtatttgcg cttacggtga
    21781 gctttttacg tgggtttgtc gccagcaaaa aaaatgcatt cagacgtcgt tttttgccgc
    21841 ctgagatttg tctattttgt gatgtgattc aaggaataca aaaagaagtg aggacaacgc
    21901 ctgatatgtg ctatcaggca ttatttgatg gattaatcct gacgaaggac tttgtgacca
    21961 taatcgagta acgcatcagt gactttacgc atcatgcggc tttcaggagc aaagcggtgc
    22021 cagtagagca tccgtcgttg aaatagccca ggcgttaagt caatcagttc accgctggcc
    22081 agctctttct cgatttgcag gtgcgggatc atacagcagg tggtgccctg gcgagcaagt
    22141 tgtacgaacg cttctgaaga attaacgata tggcagggca cgctgcctgg aggcagatcg
    22201 aagttttgct gcaaaaaggc ctggtgcata tcgtcaagat ggtcaaacgc gaccactggc
    22261 gctttcagta atgccgaacg cgttacgccg ttagggaaat atttttcggc aaagggtttt
    22321 gagctgacga acagatagtc gagcgcacca agtttatcga caagacaact cggcagcgcc
    22381 tgatgttgaa tactcaccgc gccgaccact tcgccgcggc gcagacgttc ctgagtgcgg
    22441 gtttcatctt ctacctgcaa gttgaggcgg ataggcgaat cagccaacac aggagccagt
    22501 gcaggaagca accacgtcgc cagactgtcg gcgttgaccg ccagtgaaag cagcagcgga
    22561 gtcgaaccgg tttgttcatc gcccagccac tcttcttcca gcaactccac ctggcgcagc
    22621 agtgccagca gtttttgccc ttgttccgtc gggcgcggcg gtacggtacg caccaacagc
    22681 ggctgcccga acatattttc cagttgctta atgcgctgtg agacggctga ttgtgtaatg
    22741 cacagctttt gtgccgcgcg ctcaaatcct cgttcacgta tcaccgcatc cagtgcctgt
    22801 aatgttctgt agtccgggcg tttcattgct gttgtctgct cctggtaaat gctttttcct
    22861 gcactatgac acaattttgt gtcaggttgc atataccgaa gccgtcggcg ggtaacattt
    22921 gtattcgtgg ttaaacgcgc agcttaaaag atcagggatt tgcgctaatg catttcgctc
    22981 agcgagttcg cgccctggtt gtgttgaatg gcgtggcgtt attgccccaa tttgcctgta
    23041 aacaggggct tgcgaacggt gaactggtgc gcctgtttgc accgtggagc ggcataccca
    23101 gattgttata tgccttattt gcggggcgaa aggggatgcc tgccattgcg cgatatttta
    23161 tggatgagtt aaccacgcgg cttgccaacg gggtctgaat cgcttttttt gtatataatg
    23221 cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca
    23281 gtaggatggg cggcacttca gtatgttcag cccggcacca ttgttggtgt aggtacaggt
    23341 tccaccgccg cacactttat tgacgcgctc ggtacaatga aaggccagat tgaaggggcc
    23401 gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat
    23461 ctcaacgaag tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac
    23521 atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt
    23581 gcagaaaaat ttatctgtat tgcagacgct tccaagcagg ttgatattct gggtaaattc
    23641 ccgctgccag tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa
    23701 ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg ataatggcaa cgtgatcctc
    23761 gacgtccacg gcatggaaat tcttgacccg atagcgatgg aaaacgccat aaatgcgatt
    23821 cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc
    23881 acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa
    23941 aaaattctct tcattaaatt tggtgacatg tgtcacgctt ttaccaggca attgtcgatt
    24001 gctctaaata aatcctctaa accagcatat tcatccaaga attacctttg cgtgatattt
    24061 cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa
    24121 ggcggatgca aatccgcaca caacatttca aaagacagga ttgggtaaat ggcaaaggta
    24181 tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg
    24241 gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat
    24301 gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat
    24361 ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg ctattggctg tttctgtatc
    24421 ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca
    24481 ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg
    24541 cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg
    24601 ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg
    24661 caattgggca ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaac
    24721 aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc
    24781 gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa
    24841 gagatttcgc taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg tactgtggtg
    24901 gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac
    24961 gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc
    25021 gacaacgtcc ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc
    25081 ggcctggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg
    25141 gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac
    25201 gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac
    25261 atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa
    25321 gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt
    25381 cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt
    25441 gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc
    25501 tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac
    25561 gatggcggga cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg
    25621 agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga
    25681 gcttattaac agtcagtctc aggggaggag tcatcctccc ttacccttac tcactaaatt
    25741 aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac
    25801 tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa
    25861 ttatctctgt tttgcatggg gtttttcgag cacgctcgaa gaaaagattt aaaggtagat
    25921 caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg
    25981 gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca
    26041 tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg
    26101 agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag
    26161 ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg
    26221 aagagggata tcccactctt caacggggag tttttccacc aactgacaat catgcgcata
    26281 acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc
    26341 gcccattccc aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc
    26401 taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga ttttcaacct
    26461 gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac cggcactaaa
    26521 gggatgcaaa actggaagat aaacgcgctt accggcgcgc cagagttgtt ctatgagtgg
    26581 ctgggtgtcg agttcgccat caaaagagag gaatacagcg accgtatgtg ccatcaccac
    26641 cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttcttgct gttgttccgg
    26701 cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag
    26761 tgggagttct ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc
    26821 cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa
    26881 ggcttctcgg acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg
    26941 aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg
    27001 gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg
    27061 atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt
    27121 tccatacttg ccgcgtagtc acgagtcttt gctttttctt gcgctaactc atagctgata
    27181 ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga
    27241 tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc
    27301 gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg
    27361 ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg
    27421 cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa
    27481 agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa
    27541 cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac
    27601 ccctgctgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc
    27661 atggctaccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc
    27721 acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct
    27781 ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt
    27841 ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggataa
    27901 agtgaccggc gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta
    27961 cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt
    28021 tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga
    28081 agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga
    28141 tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca
    28201 gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct
    28261 atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg
    28321 tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc
    28381 tgacggcgga gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg
    28441 gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg
    28501 gcctggatgt ggtttaccat gcccagggcg aatatgcata tgctgatgaa atcgtgaaca
    28561 gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga
    28621 tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg
    28681 tactccgccg cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc
    28741 gtccgggaat gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg
    28801 gtgcgcgcta tccgtcctat aacaccattg tcggcagcgg tgaaaacggc tgcattctgc
    28861 actacaccga aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt
    28921 gtgaatacaa aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca
    28981 cccaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc
    29041 tgtatcgtcc gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta
    29101 gcggcctggt aaaactcggc atcctgaaag gtgatgttga tgaactgatc gctcagaacg
    29161 cccatcgtcc tttctttatg catggcctta gccactggtt aggactggat gtccatgacg
    29221 ttggcgttta tggtcaggat cgctcgcgca ttctggaacc ggggatggta ctgaccgtag
    29281 agcctggtct gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc ggtatcggca
    29341 ttcgtattga agacgacatt gtgattaccg aaaccggcaa cgaaaacctc accgccagcg
    29401 tggtgaaaaa gccggaagaa atcgaagcgt tgatggcagc tgcgagaaag caatgagcgt
    29461 aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag
    29521 tcacggggcg ctgccggtac atttgattga agcgaccgcg ccagagtcac atgctcatcc
    29581 gggctttgat ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat
    29641 cggcgtctgg caatctctgg cggattacgc aaccgccatc actaccgtgc atgtcagcga
    29701 tcgtggtcac gctggatttg tcaccctcgc cgcagaagat taccaactgg cggtgctggg
    29761 acaggttgtc gaattgcaca atgtcgggca acggctgttt gcattgctgc gtaaagcacc
    29821 tggcgtaacg ctgcattgcc ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt
    29881 tgaagtgacg ctggagagtg gcgagacgct gacgggccgc gtgctggtag cagctgatgg
    29941 cacccattca gcgttagcca ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca
    30001 actggccgtg attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg
    30061 cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt
    30121 ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg
    30181 ccgtgaactc cagtcggcct ttggctggcg acttggaaaa attacccacg ccggtaaacg
    30241 cagtgcttat ccgctggcgt taacccgcgc cgccagagct attacccatc gtaccgtgct
    30301 ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat
    30361 gcgagatgtc atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat
    30421 cggtgattac ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac
    30481 catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt
    30541 cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc
    30601 gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaaa
    30661 gtgttgatgt agccattgtt ggtggcggca tggtggggct ggcggttgcc tgcggcttac
    30721 aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcgg
    30781 atgcaccacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc
    30841 gtcttggcgt ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag
    30901 tgtgggacaa agacagtttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc
    30961 atcttgggca tatcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc
    31021 agtcgtcaga taccactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa
    31081 acgaaacctt cctgacgctg aaagatggca gtatgttaac ggcgcgtctg gtgattggcg
    31141 cggacggcgc taattcctgg ttgcgcaaca aagccgatat tccgctgact ttctgggatt
    31201 atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc
    31261 ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt
    31321 gctcgattgt ctggtcactg tcgccagagg aagcgcagcg tatgcagcag gcaggggaag
    31381 acgaatttaa tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg
    31441 agagcgcgcg tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc
    31501 gtctggcgct ggtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggggtaa
    31561 atctcggctt tatggatgct gcagagctgg ttgctgaact gaaacggttg catcgtcagg
    31621 gtaaagacat cgggcagtac atttatctgc gtcgctatga gcgtagccgc aagcacagtg
    31681 cggcgatgat gctggctggt atgcagggat tccgcgatct cttttccggt accaatccgg
    31741 cgaaaaaact gctgcgtgat attggtctga aactggccga cacgcttcct ggcgttaaac
    31801 cacaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat
    31861 ttctcttctg ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg
    31921 agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat
    31981 gtcacgaatt tttgatttag tgttttttga
//
LOCUS       DAPHJE010000045        31906 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPHJE010000045 DAPHJE010000000
VERSION     DAPHJE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.045"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(213..1103)
                     /gene="rarD"
                     /locus_tag="RH222_002126"
     CDS             complement(213..1103)
                     /gene="rarD"
                     /locus_tag="RH222_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV3797142.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1155..1622)
                     /locus_tag="RH222_002127"
     CDS             complement(1155..1622)
                     /locus_tag="RH222_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV3797143.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1787..2656
                     /gene="pldA"
                     /locus_tag="RH222_002128"
     CDS             1787..2656
                     /gene="pldA"
                     /locus_tag="RH222_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV3797144.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2789..4618
                     /gene="recQ"
                     /locus_tag="RH222_002129"
     CDS             2789..4618
                     /gene="recQ"
                     /locus_tag="RH222_002129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV3797145.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4682..5302
                     /gene="rhtC"
                     /locus_tag="RH222_002130"
     CDS             4682..5302
                     /gene="rhtC"
                     /locus_tag="RH222_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV3797146.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5364..5984)
                     /gene="rhtB"
                     /locus_tag="RH222_002131"
     CDS             complement(5364..5984)
                     /gene="rhtB"
                     /locus_tag="RH222_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV3797147.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6095..7117
                     /gene="pldB"
                     /locus_tag="RH222_002132"
     CDS             6095..7117
                     /gene="pldB"
                     /locus_tag="RH222_002132"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV3797148.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7125..7925
                     /gene="yigL"
                     /locus_tag="RH222_002133"
     CDS             7125..7925
                     /gene="yigL"
                     /locus_tag="RH222_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV3797149.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTAHGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPRLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8001..8900
                     /gene="bioP"
                     /locus_tag="RH222_002134"
     CDS             8001..8900
                     /gene="bioP"
                     /locus_tag="RH222_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV3797150.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8788..9741)
                     /gene="metR"
                     /locus_tag="RH222_002135"
     CDS             complement(8788..9741)
                     /gene="metR"
                     /locus_tag="RH222_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV3797151.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9859..12120
                     /gene="metE"
                     /locus_tag="RH222_002136"
     CDS             9859..12120
                     /gene="metE"
                     /locus_tag="RH222_002136"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV3797152.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12159..12974)
                     /locus_tag="RH222_002137"
     CDS             complement(12159..12974)
                     /locus_tag="RH222_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV3797153.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13236..13997
                     /gene="udp"
                     /locus_tag="RH222_002138"
     CDS             13236..13997
                     /gene="udp"
                     /locus_tag="RH222_002138"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV3797154.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14138..15565
                     /gene="rmuC"
                     /locus_tag="RH222_002139"
     CDS             14138..15565
                     /gene="rmuC"
                     /locus_tag="RH222_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV3797155.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15660..16415
                     /gene="ubiE"
                     /locus_tag="RH222_002140"
     CDS             15660..16415
                     /gene="ubiE"
                     /locus_tag="RH222_002140"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV3797156.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16429..17034
                     /gene="ubiJ"
                     /locus_tag="RH222_002141"
     CDS             16429..17034
                     /gene="ubiJ"
                     /locus_tag="RH222_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV3797157.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17031..18671
                     /gene="ubiB"
                     /locus_tag="RH222_002142"
     CDS             17031..18671
                     /gene="ubiB"
                     /locus_tag="RH222_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV3797158.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18750..19019
                     /gene="tatA"
                     /locus_tag="RH222_002143"
     CDS             18750..19019
                     /gene="tatA"
                     /locus_tag="RH222_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV3797159.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19023..19538
                     /gene="tatB"
                     /locus_tag="RH222_002144"
     CDS             19023..19538
                     /gene="tatB"
                     /locus_tag="RH222_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV3797160.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19541..20317
                     /gene="tatC"
                     /locus_tag="RH222_002145"
     CDS             19541..20317
                     /gene="tatC"
                     /locus_tag="RH222_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV3797161.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20359..21141
                     /gene="tatD"
                     /locus_tag="RH222_002146"
     CDS             20359..21141
                     /gene="tatD"
                     /locus_tag="RH222_002146"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV3797162.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21138..21626)
                     /gene="rfaH"
                     /locus_tag="RH222_002147"
     CDS             complement(21138..21626)
                     /gene="rfaH"
                     /locus_tag="RH222_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV3797163.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21793..23286
                     /gene="ubiD"
                     /locus_tag="RH222_002148"
     CDS             21793..23286
                     /gene="ubiD"
                     /locus_tag="RH222_002148"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV3797164.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23332..24033
                     /gene="fre"
                     /locus_tag="RH222_002149"
     CDS             23332..24033
                     /gene="fre"
                     /locus_tag="RH222_002149"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV3797165.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24217..25380)
                     /gene="fadA"
                     /locus_tag="RH222_002150"
     CDS             complement(24217..25380)
                     /gene="fadA"
                     /locus_tag="RH222_002150"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV3797166.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25390..27579)
                     /gene="fadB"
                     /locus_tag="RH222_002151"
     CDS             complement(25390..27579)
                     /gene="fadB"
                     /locus_tag="RH222_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV3797167.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLQFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27769..29100
                     /gene="pepQ"
                     /locus_tag="RH222_002152"
     CDS             27769..29100
                     /gene="pepQ"
                     /locus_tag="RH222_002152"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV3797168.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29100..29714
                     /locus_tag="RH222_002153"
     CDS             29100..29714
                     /locus_tag="RH222_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV3797169.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29753..31204
                     /gene="trkH"
                     /locus_tag="RH222_002154"
     CDS             29753..31204
                     /gene="trkH"
                     /locus_tag="RH222_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV3797170.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31216..31761
                     /gene="hemG"
                     /locus_tag="RH222_002155"
     CDS             31216..31761
                     /gene="hemG"
                     /locus_tag="RH222_002155"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV3797171.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7412 a         8136 c         8726 g         7632 t
ORIGIN      
        1 ccccatcgtt aaaaaagata ataagaaagg caaaaatgaa atttattaaa ataaatgaaa
       61 atatatatcg gcgtctggaa atcttaccgt tagatgttgg gataaacata cgtaacataa
      121 attttacatc cttgtgtgag tctcctgtca gcatggcaat atgcccactc tcatacaaga
      181 gtcggcatat tttttcagaa tatatttatt ttttatttgg acgttctacg ctgcgtataa
      241 atcgcatcca tcacaaaaat tgccagcgcc acccaaataa aggcgaaagt caccatctta
      301 tcggcacccg gtttttcacc ataaaacgtc acagccagca ggaacatcag cgtcgggcca
      361 atgtactgga aaaagcctaa cgttgagaga cgcaggcgcg tggcagcggc agtaaaacac
      421 aacagcggta cggtagtgac aataccggcg gcgatcagca gtaaattcag cgacatcggg
      481 ttttgcccca tatggctggt tgagctgtcg gcaatagcaa acaggtaaat cgccgccacg
      541 ggcagcagcc acatggtttc aattaacatg ccggtttgtg cttctaccgc aatcttcttg
      601 cgcaccaggc cgtagaaggc aaaactaaat gccagcccca gcgcgataat aggtagcgaa
      661 ccaaaagtcc acagctggac taacacgcca catatcgcca gaatcaccgc cagccattgc
      721 atccggcgga atcgctcgcc gaggaaaatc atccccagca caatgttcac cagcgggtta
      781 ataaagtaac caaggctcgc ttccagcata tggtgattgt tcaccgccca gataaacagt
      841 agccagttgc caccaatcag cacggcagag actgccagca taaaaatttt ctgtggcgtc
      901 tgaatcagcg tttttaaata ggaccactgg cggcaaatgc tcatcagcac caccataaag
      961 aaaaacgacc agatcacgcg atgcgtcagg atttcatcgg cgggcacgta gtaaatcaac
     1021 ttgaagtacg ctggcgctat accccaaata aaataagcgg caagagcgag taatacgccc
     1081 tgccgcgttt gttttgcatc catcgggaat actcattttt aattggtaac agcagtttac
     1141 ctgcttttat gtcttcaacc taccatatag gtggcggtgg cactggcaat ataaagctgt
     1201 tcttcattgt gtaattcaac gcgggcgacg gcgactttat tgcctgcacg caacaggcta
     1261 ctggtagcag taaaacgctc gcccctgcct gggcgcagat aatcaacgcg aagatcaatg
     1321 gtccccatcc gcgatagccg ctggcgtagt tcatcttcac tgatggtttc gtggcgggtt
     1381 aaggtacttc ccacgcacac cagaccggcg gcgacatcca gcgccgacgc aatgactccg
     1441 ccgtgcaaaa tgctttgcgc ccagttaccc accatcatcg gctgattttt aaaggccagc
     1501 tgtgcgaact ctttttcgta acgctccagc tccatcccta acgcgcggtt aaatggcatg
     1561 tgataaacaa acatctcacc cactaatttc agggcttgtt cagcggtcag tacggcagac
     1621 atatcatcct tatacttcat tggttaatga aatgttgatt ttatgcttct ttgttgttgg
     1681 tttctacttt aggaagggat aactaacggc aatggagtta agtatgtaaa atagcccgca
     1741 gaaaaacatt caccttatca ataattcgtt acggagaaca cgaccgatgc ggactctgca
     1801 gggctggttg ttgccggtgt ttatgttgcc tatggcagta tatgcacaag aggcaacggt
     1861 gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt atcgccaata tgctgcagga
     1921 gcatgacaat ccgttcacgc tctatcctta tgacaccaac tacctcatct acacccaaac
     1981 cagcgatctg aacaaagaag cgattgccag ttacgactgg gcggaaaatg cgcgtaagga
     2041 tgaagtaaag tttcagttga gcctggcatt tccactgtgg cgtgggattt taggcccgaa
     2101 ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg caactgtcca atagcgaaga
     2161 gtcttcaccg tttcgtgaaa ccaactacga accgcaattg ttcctcggtt ttgccaccga
     2221 ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg gggtataacc acgactctaa
     2281 cgggcgttca gacccgacct cccgcagctg gaaccgcctt tatactcgcc tgatggcaga
     2341 aaacggtaac tggctggtag aagtgaagcc gtggtatgtg gtgggtaata ctgacgataa
     2401 cccggatatc accaaatata tgggttacta ccagcttaaa atcggctatc acctcggtga
     2461 tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc ggctacggcg gcgcggagtt
     2521 aggcttaagt tacccgatca ccaaacatgt gcgcctttat actcaggttt acagcggcta
     2581 tggcgaatcg ctcatcgact ataacttcaa ccagacccgt gtcggtgtgg gggttatgct
     2641 aaacgatttg ttttgatgaa cggttgagtg gttggcaaat ctggaatcca gcatccagga
     2701 ttaccctctc agagactaaa agcattgcag tttctcgcgc aggcgctgaa aatagcgcct
     2761 gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg gaagtgttga atctggagtc
     2821 cggagctaaa caggttttac aagaaacctt tggctaccaa cagtttcgcc ccggccagga
     2881 agaaattatc gacactgtgc tttccggtcg cgattgcctg gtcgtcatgc ccaccggtgg
     2941 cggaaaatcc ctttgctatc aaattcctgc cttattgcta aacggcctta ccgtggttgt
     3001 ttcaccgctg atttcgttga tgaaagatca ggtggatcaa ctgcaagcca acggcgtggc
     3061 ggcggcgtgc cttaactcga cgcaaacccg cgagcagcaa cttgaagtga tgacaggctg
     3121 tcgcaccggg caaatccgcc tgctttatat cgcgccggaa cgcctgatgc tggataactt
     3181 tcttgagcat ctggcgcact ggaatccggt gttattagcc gttgatgaag cgcactgtat
     3241 ctcccaatgg ggccacgatt tccgcccgga atatgccgcg ctcggtcagt tgcgccagcg
     3301 gttcccgacg ctgccgttta tggcgctgac cgccacagcc gacgacacca cgcgccagga
     3361 tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa atcagcagtt ttgaccgtcc
     3421 gaatattcgc tacatgctga tggagaaatt caaaccgctc gatcagttga tgcgctacgt
     3481 gcaggaacag cgcggtaagt ccggcattat ctactgcaac agccgggcga aagtagaaga
     3541 caccgctgcg cgcctgcaaa gcaagggtat tagcgcggcg gcctatcatg ccgggctgga
     3601 aaataatgtc cgcgccgacg tgcaggagaa attccagcgc gatgacctgc aaattgtggt
     3661 ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac gttcgcttcg tggtccactt
     3721 tgatattccg cgcaatatcg aatcctatta tcaggaaacc ggtcgcgccg ggcgtgatgg
     3781 cctgcctgcg gaagcgatgc tgttttacga tccggctgat atggcgtggc tgcgccgttg
     3841 tctggaagag aagccgcagg ggcagttgca ggatatcgag cgccacaaac tcaatgcgat
     3901 gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta ttgctgaact attttggcga
     3961 agggcgtcag gagccgtgcg ggaactgcga tatctgcctc gatccgccga aacagtacga
     4021 cggttcaacc gatgctcaga ttgccctttc caccattggt cgtgtgaatc agcggtttgg
     4081 gatgggttat gtggtggaag tgattcgtgg tgctaataac cagcgtatcc gcgactatgg
     4141 tcatgacaaa ctgaaagtct atggcatggg ccgtgataaa agccatgaac attgggtgag
     4201 cgtgatccgc cagctgattc acctcggcct ggtgacgcaa aatattgccc agcattctgc
     4261 cctacaactg acagaggccg cgcgcccggt gctgcgcggc gaatcctctt tgcaacttgc
     4321 cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag aaatcgttcg gcggcaacta
     4381 tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa tcgattgccg atgaaagcaa
     4441 tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt gagatggctg aacagatgcc
     4501 gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg atgcgcaagc tggaacgctt
     4561 tggtaaaccg tttatggcgc ttatccgcgc gcatgttgac ggcgacgacg aagagtagtc
     4621 agcggcataa aaaagtgcca ggatgaagac tccgtaaacg tttcccccgc gagtcaaatg
     4681 tatgttgatg ttatttctca ccgtcgccat ggtgcatatt gtggcgctta tgagccccgg
     4741 tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt tcccgtaaag aagcgatgat
     4801 gggcgtgctg ggcattacct gcggtgtaat ggtttgggct gggattgcgt tgcttggcct
     4861 gcatttgatt atcgaaaaaa tggcctggct gcatacgctg attatggttg gcggtggcct
     4921 gtatctctgc tggatgggtt accagatgct acgcggtgca ctgaaaaaag aggctgtatc
     4981 tgcgcctgca ccacagattg agctggcgaa aagcgggcgc agtttcctga aaggtttact
     5041 gaccaatctc gctaatccga aagcgattat ctactttggc tcggtgttct cattgtttgt
     5101 cggtgataac gttggcacta ccgcgcgctg gggcattttt gcgctgatca ttgtcgaaac
     5161 actggcgtgg tttaccgtcg ttgccagcct gtttgccctg ccgcaaatgc gccgtggtta
     5221 tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg ttatttgccg gatttggcat
     5281 tcatttgatt atttcgcggt gatgccagac gcgtcttcag agtaagtcgg ataaggcgtt
     5341 tacgccgcat ccgacattat ttttcacgca tgcctcgccg atgctaacag cgctcccacc
     5401 agcataaaca acgagccgaa aatcttattc agcgccttca tctgctttgg tcctttaatc
     5461 catagagcaa tccgttgagc aagggtggcg taaccgatca tcacaataat atcgactaca
     5521 atagtggtga cgccgagcac gatatactgc atcagttgcg gctgctgtgg catgatgaat
     5581 tgcggaaata gcgccgccag aaacacaata cttttgggat tggtgagatt cacaaaaact
     5641 gcgcgctgga acaaatgtcg acgcgattga gtagaggcca gcgatttaag gtcaattgca
     5701 ccagcggcgc gccactgctg gattcccagc caaatcaagt aagccgcgcc tgcccacttc
     5761 aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc ccaaccccac gccaaccagc
     5821 acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag acgccaccgc gccgcgataa
     5881 ccgtggttga gcgaggtggt catagtgttg attgcaccag agcctggcga caggcttaaa
     5941 atgatcgatg tcagcaggta ggcaaaccac cattctaagg tcatgatgaa ctcccggtgt
     6001 gtctattttt gtgccacaat acgctactgt cgcagcgttg tgtcaggcac gctaaaaaaa
     6061 acgattttac gtggtttaag aggcagatta cccgatgttt cagcagcaaa aagactggga
     6121 aacaagagaa aacgcgtttg ctgcttttac catgggaccg ctgactgatt tctggcgtca
     6181 gcgtgatgaa gcagagttta ctggtgtgga tgacattccg gtgcgctttg tccgttttcg
     6241 cgcacagcac catgaccggg tggtagtcat ctgcccgggg cgtattgaga gctacgtaaa
     6301 atatgcggaa ctggcctacg acctgttcca tttggggttt gatgtcttaa tcatcgacca
     6361 tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg catctcgggc atgttaatcg
     6421 ctttaatgat tatgttgatg atctggcggc attctggcag caggaggttc agcccggtcc
     6481 gtggcgtaaa cgctatatac tggcacattc gatgggcggt gcgatctcca cattatttct
     6541 gcaacgccat ccaggtgtat gtgacgccat tgcgctaact gcgccaatgt ttgggatcgt
     6601 gattcgtatg ccgtcattta tggcacggca gatcctcaac tgggccgaag cgcatccacg
     6661 tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc gcgttgccgt ttgctatcaa
     6721 cgtactgacc cacagcagac agcgatatcg acgtaactta cgcttctatg ctgatgaccc
     6781 aacgattcgc gtcggtgggc cgacctacca ttgggtacgc gaaagtattc tggctggcga
     6841 acaggtgtta gccggtgcgg gtgatgacgc cacgccaacg cttctcttgc aggctgaaga
     6901 ggaacgcgtg gtggataacc gcatgcatga ccgtttttgt gaactccgca ccgccgcggg
     6961 ccatcctgtc gaaggaggac gaccgttggt aattaaaggt gcttaccatg agatcctttt
     7021 tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatt gttgattttt tcaacaggca
     7081 taactcaccc agcggaaacc gctctacaga ggtttaaatt tcttatgtac caggttgttg
     7141 cgtctgattt agatggcacg ttactttctc ccgaccatac gttatcccct tacgccaaag
     7201 aaaccctgaa gctgctcacc gcgcacggca tcaactttgt gtttgcgacc ggtcgtcacc
     7261 acgttgatgt ggggcaaatt cgcgataatc tggagattaa gtcttacatg attacctcca
     7321 acggcgcgcg cgttcacgat ctggatggca atctgatttt tgctcataac ctggatcgcg
     7381 acattgccag cgatctgttt ggcgtagtca acgacaatcc ggacatcatt actaacgttt
     7441 atcgcgacga cgaatggttt atgaatcgcc atcgcccgga agagatgcgc ttttttaaag
     7501 aagcggtgtt caaatatgcg ctgtatgagc ctagattact ggagccggaa ggcgtcagca
     7561 aagtgttttt cacctgcgat tcccatgaac aactgctgcc gctggagcag gcgattaacg
     7621 ctcgttgggg cgatcgcgtc aacgttagtt tctctacctt aacctgtctg gaagtgatgg
     7681 cgggcggcgt ttcaaaaggc catgcgctgg aagcggtggc gaagaaactg ggctacagcc
     7741 tgaaggattg tattgcgttt ggtgacggga tgaacgatgc cgaaatgctg tcgatggcgg
     7801 ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa agaccttcat cccgagctgg
     7861 aagtgattgg tactaatgcc gacgacgcgg tgccgcatta tctgcgtaaa ctctatttat
     7921 cgtaatcgtt ctttatttgg tcagttgtca acctgatact tcgctacaat ggatactcgt
     7981 taatcaaaga gttttccatt gtggcgctac ttatcatcac cacgattctg tgggccttct
     8041 cctttagctt ttatggcgag taccttgcgg ggcacgtcga tagctatttt gcggtgctgg
     8101 tgcgcgttgg cctggcggca ctcgtttttc tgccgtttct gcgtacccgt ggcaatagcc
     8161 tgaaaacagt cggcctgtat atgctggtgg gcgcgatgca gctcggcgtg atgtatatgc
     8221 tgagtttccg cgcttatctc tacctgacgg tttccgaact gctgctgttc accgtactga
     8281 cgccgctcta catcacgctg atttatgaca tcatgagtaa gcgccgtctg cgctggggat
     8341 atgcttttag tgccttgttg gcggtgattg gtgccgggat tattcgctat gatcaggtca
     8401 ccgaccattt ctggactggc ttgttgctgg tgcaactctc caatatcact tttgccatcg
     8461 gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat gccgcagcat aacgcctttg
     8521 cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc atggttcttg ctgggaaatg
     8581 cgcagaaaat gccgcaaacc acgttgcaat ggggcattct ggtgtttctt ggcgtggtgg
     8641 cttccgggat tggctacttt atgtggaact acggcgcgac gcaggtggac gccggaacgc
     8701 tgggcattat gaataatatg cacgttccgg cagggctgct ggtaaacctg gctatctggc
     8761 accaacagcc gcactggcca acgttcatta caggcgcgct ggtgatcctg gcctcactgt
     8821 gggtgcatcg taagtgggtc gctccgcgct cttcacaaac ggcagatgat cgcaggcgtg
     8881 attgcgcgct gagcgaataa atgcttctgt aatcggctga cgctgctcgc catcgcgcac
     8941 ggcggcgtac agtcggctcc acaagccttc gcccagggtt ttggtcacca ccagaccctg
     9001 gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca atacccatcc gcgcggcaac
     9061 catctgaatc aacaataagg tgttatcgac gcttttcagt gacgggctga cgcctgccgg
     9121 ctgaagaaaa tgccgccaga catccagtcg actacgctgc accggataaa tcaacagcgt
     9181 ctcgctggcg agatcttccg gtgtaattcg cgttttcgcc gctagtggat ggtcaggtgc
     9241 taacaccaga cgcacttcat agtcgaacat cggcgaataa tgcaggccac tgcgcggcag
     9301 gatatcggag gtcatcacca gatccagctc gccctgttgc aacgcgggct gcgggtcaaa
     9361 cgtcacgccc gatttaaaat ccatctctac ctgcggccag ttcttatgga aattttctaa
     9421 cgcgggcgtc agccactgaa tacagctatg gcactcaatg gcaatgcgca gacgcgtctg
     9481 ctgcggttca ttgcaggctt gcagtgcctg gctaatttgc ggcagcacct ggtttgccag
     9541 ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc tggctcttac gcacaaatag
     9601 ccggaagcca aggcgttgtt ccagatcgct aaactggtga gacagggcgg attgcgtctg
     9661 atgcaacgtc gccgcagcgg ctgcgagaga gccgcagttc cgcaacgctt gtagcgtttt
     9721 caggtgtttt acttcgatca tgaaagtcct tcacttcggc atgaataatt tgcgcttgag
     9781 gaatatacag taaccgccaa ttatggatgt gtaaacatct ggacggctaa atactaaaaa
     9841 ttccataagg ggcatataat gacaattctt aatcacaccc tcggtttccc tcgcgttggc
     9901 ctgcgtcgcg agctgaaaaa agcgcaagaa agttattggg cggggaactc cacgcgtgaa
     9961 gaactgctga cagtagggcg tgaactgcgt gctcgtcact gggatcaaca aaagcaagcg
    10021 ggtatcgacc tgctgccggt gggcgatttt gcctggtacg atcatgttct gaccaccagt
    10081 ctgctgctgg gtaatgttcc gccacgtcat cagaacaaag acggttcggt agatatcgac
    10141 accctgttcc gtattggtcg tggacgtgcg ccgactggcg aacctgcggc ggcagcggaa
    10201 atgaccaaat ggtttaacac caactatcac tacatggtgc cggaattcgt taaaggccaa
    10261 cagttcaaac tgacctggac gcagctgctg gaggaagtgg acgaggcgct ggcgctgggc
    10321 cacaacgtta agcctgtgct gctggggccg gttacttacc tgtggctggg gaaagtgaaa
    10381 ggtgaacagt ttgaccgcct tagcctgctg aacgacattc tgccggttta tcagcaagtg
    10441 ctggcagaac tggcgaaacg cggcatcgag tgggtacaga ttgatgaacc cgcgctggta
    10501 ctggaactac cacaggcgtg gctggacgca tacaaacccg cttacgacgc gctccaggga
    10561 caggtgaaac tgctgctgac cacctatttt gaaggcgtaa cgccaaatct cgacacgatt
    10621 actgcgctgc ctgttcaggg tctgcatgtt gaccttgtac atggtaaaga tgacgttgtt
    10681 gaactgcaca agcgcctgcc ttctgactgg ctgctgtctg cgggactgat caatggtcgt
    10741 aacgtctggc gcgccgatct tactgagaaa tatgcgcaaa ttaaggacat tgtcggcaaa
    10801 cgtgatttgt gggtggcatc ttcctgctcg ttgctgcaca gccccatcga cctgagcgtg
    10861 gaaacgcgtc ttgatgcaga agtgaaaagc tggtttgcct tcgctctaca aaaatgccat
    10921 gaactggcac tgctgcgcga tgcgctgaac agtggtgaca cggcagctct ggcagagtgg
    10981 agcgccccaa ttcaggcgcg tcgtcactct actcgtgtac ataatccggc ggtagaaaag
    11041 cgtctggcgg cgatcaccgc ccaggacagc cagcgtgcga atgtctatga agtgcgtgct
    11101 gaagcccagc gtgcgcgttt taaactgcca gcgtggccga ccaccacgat tggttccttc
    11161 ccgcaaacca cggaaattcg taccctgcgt ctggatttca aaaagggcaa tctcgacgcc
    11221 aacaactacc gcacgggcat tgcggaacat atcaagcagg ccattgttga gcaggaacgt
    11281 ttgggactgg atgtgctggt acatggcgag gccgagcgta atgacatggt ggaatacttt
    11341 ggcgagcacc tcgacggatt tgtctttacg caaaacggtt gggtacagag ctacggttcc
    11401 cgctgcgtga agccaccgat tgtcattggt gacattagcc gcccggcacc gattaccgtg
    11461 gagtgggcga agtatgcgca atcgctgacc gacaaaccgg tgaaagggat gctgacgggg
    11521 ccggtgacca tactctgctg gtcgttcccg cgtgaagatg tcagtcgtga aaccatcgcc
    11581 aaacagattg cgctggcgct gcgtgatgag gtggccgatc tggaagcggc agggattggc
    11641 atcatccaga ttgatgaacc ggcgctgcgc gaaggtttac cgctgcgtcg tagcgactgg
    11701 gacgcctatc tccagtgggg cgtagaggcc ttccgtatca acgccgccgt ggcgaaagat
    11761 gacacacaaa tccacactca catgtgttat tgcgagttca acgacatcat ggattcgatt
    11821 gcggcgctgg acgcagacgt catcaccatc gaaacctcgc gttccgacat ggagttgctg
    11881 gagtcgtttg aagagtttga ttatccaaat gaaatcggtc ctggcgtcta tgacattcac
    11941 tcgccaaacg taccgagcgt ggaatggatt gaagccttgc tgaagaaagc ggcaaaacgc
    12001 attccggcag agcgcctgtg ggtcaacccg gactgtggcc tgaaaacgcg cggctggcca
    12061 gaaacccgcg cggcactggc gaacatggtg caggcggcgc agaatttgcg tcgggggtaa
    12121 atccaaaccg ggtggtaata ccacccggtc ttttctcatt acagcgactt cttcccacca
    12181 tactgcttaa accactccag catacgctgc cagccatctt tcgcagattc ggcatgatag
    12241 ctcgggcgat aatcggcgtt gaatgcatgc ccggcatccg ggtagacgat aatctctgct
    12301 ttcgcattag cagcccgcag cgcctggcgc atggtttcaa cgctctcctg cggaatgctg
    12361 ttatcctgac caccatataa gccgagaacc ggcgcgttaa gatcggttgc gatatcaaca
    12421 ggttgtttcg gtgaattcag cgacttgtcg cccgtcagtt tgccgtacca cgccactgca
    12481 gcttttagct gtggattatg cgcggcatac agccaggtga tacgtccacc ccagcagaat
    12541 ccggtgatca ttaaacgatg aacatcgccg ccgttgcggg aagcccaact ggcgacatga
    12601 tcgagatcgg ccagcacctg cgagtcaggc acttttgcta ccagaccgct aagcaacgtg
    12661 gggatatcgg caaaatcatt cggatcgcct tcgcggaagt aaagttcagg tgcgatagcc
    12721 agatacccct ccagcgccag acggcgacaa atgtcgcgga tatgttcatg cacgccaaaa
    12781 atttcctgca ctacaatgac cactggcagt gggccatcgc tttgctttgg tctggcatgg
    12841 taagcaggca tgttatcccc ttgtgaaggg atagaggtga agcccgccac aatcgcgtcg
    12901 tccggggtct gaacgatggt cgaagcgaga ggcgatgcag caggtgcaaa tccagattgt
    12961 tgtgttgttg ccatggtatt ctccgtaccc ttataaaaat gttgcgcaat gttaactata
    13021 gtcagcatgc aacaaatcac attgcctgaa tcggctcatc ttttatgcag tcctgcagaa
    13081 tgaagagtga tttatgtgat ttgaatcact tttggtgggt aaatttatgc aacgcatttg
    13141 cgtcatggtg atgagtatca cgaaaaaatg ttaaaccctt cggtaaagtg tctttttgct
    13201 tcttctgact aaaccgattc acagaggagt tgtatatgtc caagtctgat gtttttcatc
    13261 tcggcctcac taaaaacgat ttacaagggg ctacgcttgc catcgtccct ggcgacccgg
    13321 atcgtgtgga aaagatcgcc gcgctgatgg ataagccggt taagctggca tctcaccgcg
    13381 aattcactac ctggcgtgca gagctggatg gtaaacctgt tatcgtctgc tctaccggta
    13441 tcggcggccc gtctacctct attgctgttg aagagctggc acagctgggc attcgcacct
    13501 tcctgcgtat cggtacaacg ggcgctattc agccgcatat taatgtgggt gatgtcctgg
    13561 ttaccacggc gtctgtccgt ctggatggcg cgagcctgca cttcgcaccg ctggaattcc
    13621 cggctgtcgc tgatttcgaa tgtacgactg cgctggttga agctgcgaaa tccattggcg
    13681 cgacaactca cgttggcgtg acagcttctt ctgatacctt ctacccaggt caggaacgtt
    13741 acgatactta ctctggtcgc gtagttcgtc actttaaagg ttctatggaa gagtggcagg
    13801 cgatgggcgt aatgaactat gaaatggaat ctgcaaccct gctgaccatg tgtgcaagtc
    13861 agggcctgcg tgccggtatg gtagcgggtg ttatcgttaa ccgcacccag caagagatcc
    13921 cgaatgctga gacgatgaaa caaactgaaa gccaagcggt gaaaatcgtg gtggaagcgg
    13981 cgcgtcgtct gctgtaattc tcttctcctg tctgaaggcc gacgcgttcg gccttttgta
    14041 tttttgcgta gcgcctcgca ggaaatgcct ttccaactgg acgtttgtac agcacaattc
    14101 tattttgtgc gggtaagttg ttgcgtcagg aggcgttgtg gatttctcaa tcatggttta
    14161 cgcagttatt gcgttggtgg gtgtggcaat tggctggctg tttgccagtt atcaatatgc
    14221 gcagcaaaaa gccgagcaat tagctgaacg tgaagagatg gtcgcggagt taagcgcggc
    14281 aaaacaacaa attacccaaa gcgagcactg gcgtgcagag tgcgagttac tcaataacga
    14341 agtgcgcagc ctgcaaagta ttaacacctc tctggaggcc gatctgcgtg aagtaaccac
    14401 gcggatggaa gccgcacagc aacatgctga cgataaaatt cgccagatga ttaacagcga
    14461 gcagcgcctc agtgagcagt ttgaaaacct cgccaaccgt atttttgagc acagcaatcg
    14521 ccgggttgat gagcaaaacc gtcagagtct gaacagcctg ttgtcgccgc tacgtgaaca
    14581 actggacggt ttccgccgtc aggttcagga cagcttcggt aaagaagcac aagaacgcca
    14641 taccctgacc cacgaaattc gcaatctcca gcaactcaac gcgcaaatgg cccaggaagc
    14701 gatcaacctg acgcgcgcgc tgaaaggcga caataaaacc cagggcaact ggggcgaggt
    14761 agtattgacg cgggtgctgg aggcttccgg tctgcgtgaa gggtatgaat atgaaaccca
    14821 ggtcagcatc gaaaatgacg cccgctcgcg gatgcagccg gatgtcatcg tgcggctgcc
    14881 gcagggaaaa gatgtggtga tcgacgccaa aatgacgctg gtcgcctatg aacgctattt
    14941 taacgccgaa gacgactaca cccgcgaaag cgcgctacag gaacatatcg cgtcggtgcg
    15001 taaccatatc cgtttgctgg gacgcaaaga ttatcaacag ctgccggggc tgcgaactct
    15061 ggattacgtg ctgatgttta ttcccgttga acccgctttt ttactggcgc ttgaccgcca
    15121 gccggagctg atcaccgaag cgttgaaaaa caatatcatg ctggttagcc cgactacgct
    15181 gctggtggcg ttgcgtacta ttgccaacct gtggcgttat gagcatcaaa gccgcaacgc
    15241 ccagcaaatc gccgatcgtg ccagcaagct gtacgacaag atgcgtttgt tcatcgatga
    15301 catgtccgcg attggtcaaa gtctcgacaa agcgcaggat aactaccgcc aggcaatgaa
    15361 aaaactctct tcaggtcgcg gaaatgtgct ggcgcaggca gaagcgtttc gcggtttagg
    15421 agtagaaatt aaacgcgaga ttaatccgga tttggctgaa caggcggtga gccaggatga
    15481 agagtatcga cttcggtcgg ttccggagca gccgaatgat gaagcttatc aacgcgatga
    15541 tgaatataat cagcagtcgc gctagcccat taggggtagt taagccgggt agaaatctag
    15601 ggcatcgacg cccaatctgt tacacttctg gaacaatttt ttgatgagca ggcattgaga
    15661 tggtggataa gtcacaagaa acgacgcact ttggttttca gaccgtcgcg aaggaacaaa
    15721 aagcggatat ggtcgcccac gttttccatt ccgtggcatc aaaatacgat gtcatgaatg
    15781 atttgatgtc atttggtatt catcgtttgt ggaagcgatt cacgattgat tgcagcggcg
    15841 tacgccgtgg gcagaccgtg ctggatctgg ctggtggcac cggcgacctg acagcgaaat
    15901 tctcccgcct ggtcggagaa actggcaaag tggtccttgc tgatatcaat gaatccatgc
    15961 tcaaaatggg ccgcgagaag ctgcgtaata tcggtgtgat tggcaacgtt gagtatgttc
    16021 aggcgaacgc tgaggcgctg ccgttcccgg ataacacctt tgattgcatc accatttcgt
    16081 ttggtctgcg taacgtcacc gacaaagata aagcactgcg ttcaatgtat cgcgtgctga
    16141 aacccggcgg ccgcctgctg gtgcttgagt tctcgaagcc aattatcgag ccgctgagca
    16201 aagcctatga tgcatactcc ttccatgtgc tgccgcgtat tggctcactg gtcgcgaacg
    16261 acgccgacag ctaccgttat ctggcagaat ccatccgtat gcatcccgat caggataccc
    16321 tgaaagccat gatgcaggat gccggattcg aaagtgtcga ctactacaat ctgacggcag
    16381 gggttgtggc gctgcatcgt ggttataagt tctgacagga gaccggaaat gccttttaaa
    16441 cctttagtga cggcaggaat tgaaagtctg ctcaacacct tcctgtatcg ctcacccgcg
    16501 ctgaaaacgg cccgctcgcg tctgctgggt aaagtattgc gcgtggaggt aaaaggcttt
    16561 tcgacgtcat tgattctggt gttcagcgaa cgccaggttg atgtactggg cgaatgggca
    16621 ggcgatgctg actgcaccgt tatcgcctac gccagtgtgt tgccgaaact tcgcgatcgc
    16681 cagcagctta ccgcactgat tcgcagtggt gagctggaag tgcagggcga tattcaggtg
    16741 gtgcaaaact tcgttgcgct ggcagatctg gcagagttcg accctgcgga actgctggcc
    16801 ccttataccg gtgatatcgc cgctgaagga atcagcaaag ccatgcgcgg aggcgcaaag
    16861 ttcctgcatc acggcattaa gcgccagcaa cgttatgtgg cggaagccat tactgaagag
    16921 tggcgtatgg cacccggtcc gcttgaagtg gcctggtttg cggaagagac ggctgccgtc
    16981 gagcgtgctg ttgatgccct gaccaaacgg ctggaaaaac tggaggctaa atgacgccag
    17041 gtgaagtacg gcgcctatat ttcatcattc gcactttttt aagctacgga cttgatgaac
    17101 tgatccccaa aatgcgtatc accctgccgc tacggctatg gcgatactca ttattctgga
    17161 tgccaaatcg gcataaagac aaacttttag gtgagcgact acgactggcc ctgcaagaac
    17221 tggggccggt ttggatcaag ttcgggcaaa tgttatcaac ccgccgcgat ctttttccac
    17281 cgcatattgc cgatcagctg gcgttattgc aggacaaagt tgctccgttt gatggcaagc
    17341 tggcgaagca gcagattgaa gctgcaatgg gcggcttgcc ggtagaagcg tggtttgacg
    17401 attttgaaat caagccgctg gcttctgctt ctatcgccca ggttcatacc gcgcgattga
    17461 aatcgaatgg taaagaggtg gtgattaaag tcatccgccc ggatattttg ccggttatta
    17521 aagcggatct gaaacttatc taccgtctgg ctcgctgggt gccgcgtttg ctgccggatg
    17581 gtcgccgtct gcgcccaacc gaagtggtgc gcgagtacga aaagacattg attgatgaac
    17641 tgaatttgct gcgggaatct gccaacgcca ttcagcttcg gcgcaatttt gaagacagcc
    17701 cgatgctcta catcccggaa gtttaccctg actattgtag tgaagggatg atggtgatgg
    17761 agcgcattta cggcattccg gtgtctgacg ttgcggcgct ggagaaaaac ggcactaaca
    17821 tgaaattgct ggcggaacgc ggcgtgcagg tgttcttcac tcaggtcttt cgcgacagct
    17881 ttttccatgc cgatatgcac cctggcaaca tcttcgtaag ctatgaacac ccggaaaacc
    17941 cgaaatatat cggcattgat tgcgggattg ttggctcgct aaacaaagaa gataaacgct
    18001 atctggcaga aaactttatc gccttcttta atcgcgacta tcgcaaagtg gcagagctac
    18061 acgtcgattc aggctgggtg ccaccagata ccaacgttga agagttcgaa tttgccattc
    18121 gtacggtctg tgaacctatc tttgagaaac cgctggccga aatttcgttt ggacatgtac
    18181 tgttaaatct gtttaatacg gcgcgtcgct tcaatatgga agtgcagccg caactggtgt
    18241 tactccagaa aaccctgctc tacgtcgaag gggtaggacg ccagctttat ccgcaactcg
    18301 atttatggaa aacggcgaag cctttcctgg agtcgtggat taaagatcag gtcggtattc
    18361 ctgcgctggt gagagcattt aaagaaaaag cgccgttctg ggtcgaaaaa atgccagaac
    18421 tgcctgaact ggtttacgac agtttgcgcc agggcaagta tttacagcac agtgttgata
    18481 agattgcccg cgagcttcag tcaaatcatg tacgtcaggg acaatcgcgt tattttctcg
    18541 gaattggcgc tacgttagta ttaagtggca cattcttgtt ggtcagccga cctgaatggg
    18601 ggctgatgcc cggctggtta atggcaggtg gtctgatcgc ctggtttgtc ggttggcgca
    18661 aaacacgctg attttttcat cgctcaaggc gggccgtgta acgtataatg cggccttgtt
    18721 taatcatcat ctaccacaga ggaacatgta tgggtggtat cagtatttgg cagttattga
    18781 ttattgccgt catcgttgta ctgctttttg gcaccaaaaa gctcggctcc atcggttccg
    18841 atcttggtgc gtcgatcaaa ggctttaaaa aagcaatgag cgatgatgaa ccaaagcagg
    18901 ataaaaccag tcaggatgct gattttactg cgaaaactat cgccgataag caggcggata
    18961 cgaatcagga acaggctaaa acagaagacg cgaagcgcca cgataaagag caggtgtaat
    19021 ccgtgtttga tatcggtttt agcgaactgc tattggtgtt catcatcggc ctcgtcgttc
    19081 tggggccgca acgactgcct gtggcggtaa aaacggtagc gggctggatt cgcgcgttgc
    19141 gttcactggc gacaacggtg cagaacgaac tgacccagga gttaaaactc caggagtttc
    19201 aggacagtct gaaaaaggtt gaaaaggcga gcctcactaa cctgacaccc gaactgaaag
    19261 cgtcgatgga tgaactacgc caggccgcgg agtcgatgaa gcgttcctac gttgcaaacg
    19321 atcctgaaaa ggcgagcgat gaagcgcaca ccatccataa cccggtggtg aaagataatg
    19381 aagctgcgca tgagggcgta acgcctgccg ctgcacaaac gcaggccagt tcgccggaac
    19441 agaagccaga aaccacgcca gagccggtgg taaaacctgc tgcggacgct gaaccgaaaa
    19501 ccgctgcacc ttccccttcg tcgagtgata aaccgtaaac atgtctgtag aagatactca
    19561 accgcttatc acgcatctga ttgagctgcg taagcgtctg ctgaactgca ttatcgcggt
    19621 gatcgtgata ttcctgtgtc tggtctattt cgccaatgac atctatcacc tggtatccgc
    19681 gccattgatc aagcagttgc cgcaaggttc aacgatgatc gccaccgacg tggcctcgcc
    19741 gttctttacg ccgatcaagc tgacctttat ggtgtcgctg attctgtcag cgccggtgat
    19801 tctctatcag gtgtgggcat ttatcgcccc agcgctgtat aagcatgaac gtcgcctggt
    19861 ggtgccgctg ctggtttcca gctctctgct gttttatatc ggcatggcat tcgcctactt
    19921 tgtggtcttt ccgctggcat ttggcttcct tgccaatacc gcgccggaag gggtgcaggt
    19981 atccaccgac atcgccagct atttaagctt cgttatggcg ctgtttatgg cgtttggtgt
    20041 ctcctttgaa gtgccggtag caattgtgct gctgtgctgg atggggatta cctcgccaga
    20101 agacttacgc aaaaaacgcc cgtatgtgct ggttggtgca ttcgttgtcg ggatgttgct
    20161 gacgccgccg gatgtcttct cgcaaacgct gttggcgatc ccgatgtact gtctgtttga
    20221 aatcggtgtc ttcttctctc gcttttacgt tggtaaaggg cgaaatcggg aagaggaaaa
    20281 cgacgctgaa gcagaaagcg aaaaaactga agaataaatt caaccgcccg tcagggcggt
    20341 tgtcatatgg agtacaggat gtttgatatc ggcgttaatt tgaccagttc gcaatttgcg
    20401 aaagaccgtg atgatgttgt agcgcgcgct tttgacgcgg gagttaatgg gctactcatc
    20461 accggcacta acctgcgtga aagccagcag gcgcaaaagc tggcgcgtca gtattcgtcc
    20521 tgttggtcaa cggcgggcgt acatcctcac gacagcagcc agtggcaagc tgcgactgaa
    20581 gaagcgatta ttgagctggc cgcgcagcca gaagtggtgg cgattggtga atgtggtctc
    20641 gactttaacc gcaacttttc gacgccggaa gagcaggaac gcgcttttgt tgcccagcta
    20701 cgcattgccg cagatttaaa catgccggta tttatgcact gtcgcgatgc ccacgagcgg
    20761 tttatgacat tgctggagcc gtggctggat aaactgcctg gtgcggttct tcattgcttt
    20821 accggcacac gcgaagagat gcaggcgtgc gtggcgcatg gaatttatat cggcattacc
    20881 ggttgggttt gcgatgaacg acgcggactg gagctgcggg aacttttgcc gttgattccg
    20941 gcggaaaaat tactgatcga aactgatgcg ccgtatctgc tccctcgcga tctcacgcca
    21001 aagccatcat cccggcgcaa cgagccagcc catctgcccc atattttgca acgtattgcg
    21061 cactggcgtg gagaagatgc cgcatggctg gctgccacca cggatgctaa tgtcaaaaca
    21121 ctgtttggga ttgcgtttta gagtttgcgg aactcggtgt tcttcacact gtgcttaatc
    21181 tctttattaa taagattaag caatagcatg gagcgagcct caccatcggg ttcggtgaaa
    21241 atggcctgaa agccttcgaa cgcgccttcg gtaataatca ccttatcacc cggataaggg
    21301 gttgccggat cgacaatgtc tttcggttta tataccgata gctgatgaat caccgccgat
    21361 gggactatcg ctggcgacgc gccaaagcgc acgaagtggc tgacaccgcg ggtcgcgttg
    21421 atagtcgtgg tatgaatcac ttctggatca aattcgacaa acaggtagtt gggaaacaat
    21481 ggctcactga ctgcagtacg ttttccacgc acgatttttt ccagggtgat catcggtgcc
    21541 aggcaattca cagcctgtct ttcgaggtgt tcctgggcac gttgaagttg cccgcgcttg
    21601 cagtacagta aataccagga ttgcataatg actcttatcc gtttaatcgg ggcgcaagga
    21661 tagcaaaagc tttacgctaa gttaattata ttccccggtt tgcgttatac cgtcagagtt
    21721 cacgctaatt taacaaattt acagcatcgc aaagatgaac gccgtataat gggcgtagat
    21781 taagaggcta caatggacgc catgaaatat aacgatttac gcgacttcct gacgctgctt
    21841 gaacagcagg gtgagctaaa acgtatcacg ctcccggtgg atccgcatct ggaaatcact
    21901 gaaattgctg accgcacttt gcgtgccggt gggcctgcgc tgttgttcga aaaccctaaa
    21961 ggctactcaa tgccggtgct gtgcaacctg ttcggtacgc caaagcgcgt ggcgatgggc
    22021 atggggcagg aagatgtttc ggcgctgcgt gaagttggta aattattggc gtttctgaaa
    22081 gagccggagc cgccaaaagg tttccgcgac ctgtttgata aactgccgca gtttaagcaa
    22141 gtattgaaca tgccgacaaa gcgactgcgt ggtgcgccct gccaacaaaa aatcgtctct
    22201 ggcgatgacg tcgatctcaa tcgcattccc attatgacct gctggccgga agatgccgcg
    22261 ccgctgatta cctgggggct gaccgtaacg cgcggcccgc ataaagagcg gcagaatctg
    22321 ggcatttatc gtcagcagct gattggtaaa aacaaactga ttatgcgctg gctgtcgcat
    22381 cgcggcggcg cgctggatta tcaggagtgg tgtgcggcgc atccgggcga acgtttcccg
    22441 gtttctgtgg cgctgggtgc cgatcccgcc acgattctcg gtgcagtcac ccccgttccg
    22501 gatacgcttt cagagtatgc gtttgccgga ttgctacgtg gcaccaagac cgaagtggtg
    22561 aagtgtatct ccaatgacct tgaagtgccc gccagtgcgg agattgtgct ggaagggtat
    22621 atagaacaag gcgaaactgc tccggaaggg ccgtatggcg accacaccgg ttactataac
    22681 gaagtcgata gtttcccggt atttaccgtg acgcatatta cccagcgtga agatgcgatt
    22741 taccactcca cctatactgg tcgtccgccg gatgaacccg cggtgctggg tgtcgcactg
    22801 aacgaagtgt ttgtgccgat tctgcaaaaa cagttcccgg aaattgtcga tttttacctg
    22861 ccgccggaag gctgttctta tcgtctggcg gtagtgacga taaaaaaaca gtacgccgga
    22921 cacgcgaagc gcgtcatgat gggcgtctgg tcgttcttac gccagtttat gtacactaag
    22981 tttgtgatcg tttgcgatga tgacgtcaac gcacgcgact ggaacgatgt gatttgggcg
    23041 attaccaccc gtatggatcc ggcgcgggat actgttctgg tagaaaatac gcctattgat
    23101 tatctggatt ttgcctcgcc tgtttccggg ctgggttcaa aaatggggct ggatgccacg
    23161 aataaatggc cgggggaaac ccagcgtgaa tggggacgtc ccatcaaaaa agatccagat
    23221 gttgtcgcgc atattgacgc catctgggat gaactggcta tttttaacaa cggtaaaagc
    23281 gcctgatgtg cgtttgtttt gccctattta tcgatccgac agagaaagcg catgacaacc
    23341 ttaagctgta aagtgacctc ggtagaagct atcacggata ccgtatatcg tgtccgcatc
    23401 gtgccagacg cggccttttc ttttcgtgct ggtcagtatt tgatggtagt gatggatgag
    23461 cgcgacaaac gtccgttctc aatggcctcg acgccggatg aaaaagggtt tatcgagctg
    23521 catattggcg cttctgaaat caacctttac gcgaaagcag tcatggaccg catcctcaaa
    23581 gatcatcaaa tcgtggtcga tattccccac ggagaagcgt ggctgcgcga tgatgaagag
    23641 cgtccgatga ttttgattgc gggcggcacc gggttctctt atgcccgctc gattttgctg
    23701 acagcgttgg cgcgtaaccc aaaccgtgat atcaccattt actggggcgg gcgtgaagag
    23761 cagcatctgt atgatctctg cgagcttgaa gcgctttcgt taaagcaccc tggtctgcaa
    23821 gtggtgccgg tggttgaaca accggaagcg ggctggcgtg ggcgtactgg caccgtgtta
    23881 acggcggtat tgcaggatca cggtacgctg gcagagcatg atatctatat tgccggacgt
    23941 tttgagatgg cgaaaatagc ccgtgacctg ttttgtagtg agcgtaatgc gcgggaagat
    24001 cgcctgtttg gcgatgcgtt tgcatttatc tgagatataa aaaaacccgc ccctgacagg
    24061 cgggaagaac ggcaactaaa actttattca gtggcattta gatctatgac gtatctggca
    24121 aaccatgccc gatgcgacgc tgtcgcgtct tatcgtgcct acaaatagtc cgaaccgtag
    24181 gccggataag gcgtttacgc cgcatccggc aagtggttaa acccgctcaa acaccgtcgc
    24241 aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt ctttgcgttc
    24301 catcagattc agcagcgtgg tgctgatacg cgcaccggaa cagcccagcg gatgacccag
    24361 cgcgatcgca ccaccattga ggttgatctt ctcgtcaatc tgctccatta gcccgagatc
    24421 tttaatgcac ggcaggatct gcgcggcaaa ggcttcgttc atctcaaaca cgccgatatc
    24481 gctggcagaa agccccgctt ttttcagcgc cagcttcgag gccggaaccg ggccgtaacc
    24541 cataatcgat gggtcacaac caacgaccgc catcgaacgc acacgagcgc gcggcttaag
    24601 acctaattca tgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag
    24661 tgcagaagat gtgcccgccg ttaccgtacc gtttactgga tcaaacgccg gacgcagcgt
    24721 ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcag
    24781 gacgccgtcg gcatcgtgac caccagttgg gatgatttca tttttaaatg cggccgactg
    24841 cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct
    24901 gataccgtgc atacgcgcca gcatttctgc tgttaagccc atcatgcccg ccgctttggc
    24961 gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca catggcccat
    25021 atgctccacg ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc
    25081 gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg ccgggacaga
    25141 gtgcggcact tctgccagca gcgccgcgtt acgggcgatg ttaaaaccct gctccagcgt
    25201 ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg ccgggttacg
    25261 cgccagcagg ctacgcatta aatgagcgga gagatcttcc gcacgcacgt tacgaaaagc
    25321 accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat
    25381 tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata
    25441 gtacggttcg ttatgacgtg ctttattacg cagaccttcc ggcacttcgt acagcgggcc
    25501 gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg tgtccagcca
    25561 gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg ccatatccgc
    25621 ttccgccgga gtggcgataa tgccttcctc cagacagcgc accacttcgt tgaccatcgg
    25681 gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct ggctcacttc
    25741 tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt tgctgtcttc
    25801 tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat caaacagcgc
    25861 gtcgatggca tcgcggtaat ctttctgcat ccgctgcggg aagcctgctg ccatgacagc
    25921 ctgagcgtga tgcgcggtat caatgcccac gacatccagc agatatgccg gacccatagg
    25981 ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag
    26041 cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga agccggggca
    26101 gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa ctttcgcgat
    26161 ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcatcc ggtgtaccgg
    26221 gttaaaaaag tgcattccgc agaagttttc cgggcgttcc agcgcgttgg ccagttcgct
    26281 gataggaatg gttgaagtgt tagacgccag cacggtatcc gggcgtactt tttgttcggt
    26341 ttctgccagt acggcttttt tcactttcgg gttttcgaca accgcttcta ccacaacatc
    26401 cacgcggtca aacccggcgt agtcgagcgt tgggtggatt gtggagatca cgccagccag
    26461 tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg cggcttcggt
    26521 catgccgagg gttaacgact tgtcgttgat atctttcatg acaaccggca cgcctttcca
    26581 cgcagactgg taagcgatgc cgccgcccat aatgcctgca cccagcaccg cggcctgttt
    26641 cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag
    26701 gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc agcgggacaa aacttttgtt
    26761 ttccaggttt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc
    26821 ggtgatgggg gccggataat gtttccccgc tgtttgtgcg accatccctt tagcgatggt
    26881 gaagctcatg gtggcttcaa tcttgctcag ttttagtggt tccagcttcg gctgacgttt
    26941 tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa accgcctttg cgccttcaac
    27001 cagtttttct gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc
    27061 gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca tacgtggcat
    27121 acgtacagaa ccgccaaagc ccggcatgat acccagtttg gtttccggca gaccgatgcg
    27181 cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag
    27241 cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac
    27301 gctattggca aattgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa
    27361 aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca gcagcagccc
    27421 ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc
    27481 agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa tgccatcttc
    27541 cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa
    27601 ggtgatctgg tcgtaccaga tgagtcgaag tgtgtatttt gtgttaaaaa tatgcaaaca
    27661 aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga
    27721 tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg
    27781 gcctcgctct ataaaaatca tatagctacc ttacaagaac ggactcgcga tgcgctggcg
    27841 cgcttcaagc tggatgcgtt acttattcac tccggcgagc tgttcaatgt ttttctcgac
    27901 gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag
    27961 gtgccaaact gctggttgct ggtggatggc gtgaacaagc cgaaactgtg gttttatctg
    28021 ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac tgaagatgtg
    28081 gaagtgatcg cgctgccgaa agccgatggc attggtagcc tgctgcctgc tgcgcgcggc
    28141 aatatcggtt atatcggtcc ggtgccggag cgtgcgctgc aactgggtat tgaggccagc
    28201 aacatcaacc cgaaaggggt tatcgactac ctgcattact atcgctcctt caaaaccgag
    28261 tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgtgcggca
    28321 gaagaagcgt tccgttctgg catgagcgag ttcgatatca acattgccta tctgaccgcg
    28381 accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa cgaacacgct
    28441 gcggtgctgc attacaccaa actggatcat caggcgccgg aagagatgcg cagcttcctg
    28501 ctggatgccg gggccgaata taacggctat gcggctgacc tgactcgtac ctggtcggca
    28561 aaaagcgaca acgactacgc acagctggtg aaagacgtaa atgatgaaca actggcgctg
    28621 atcgccacca tgaaagcagg cgttagctat gtggattacc acatccagtt ccatcagcgc
    28681 atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc
    28741 gaaaacgatc tcaccggacc gtttatgccg cacggtatcg gtcatccgct gggcctgcag
    28801 gtgcatgacg tagccggttt tatgcaggat gatagcggta cacacctcgc ggcaccggca
    28861 aaatatccgt acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa
    28921 ccgggtatct acttcattga atcgctactg gcaccgtggc gtgaagggca gttcagcaag
    28981 cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac
    29041 aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga
    29101 tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc
    29161 gcttcataac actgttggcg cataccgatg gcgttgaggc ggcgaaagcg tttgttgaat
    29221 cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg gcgggtgcgc
    29281 cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcagga acggcaggta
    29341 aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct gtggtagtgc
    29401 gctactacgg tggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg
    29461 tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac gccattaacc gaatatactt
    29521 tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca
    29581 aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga
    29641 aagtggctga attttccgca aagctggcgg attttagccg tggttcattg caattgttag
    29701 cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt
    29761 tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat
    29821 ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt
    29881 ttttgtcgcc ctcgccattg gttctatgct gtggtggccg aaccgtaaag agaagggcga
    29941 actgaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagtgt
    30001 cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt
    30061 tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc tggattcgct
    30121 ccctcacgcc atcctctttt atcgccagat gctgcaatgg tttggcggga tggggatcat
    30181 cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc
    30241 agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacggcgaa
    30301 aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc
    30361 cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta ttggcggctt
    30421 ctcgacacat gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc
    30481 tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt taagtgggcg
    30541 tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagtttac
    30601 gctggtggtt atttgtaccc tcgtactgtg gtttcacaat gtctacagtt cggcgctgat
    30661 gacaattaac caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacgac
    30721 tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat
    30781 cggcggttgt gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt
    30841 taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa
    30901 gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc
    30961 ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt
    31021 ttctgctttt gcctcggttg ttgcgacatt gaataacctg gggccggggc ttggcgtggt
    31081 tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct
    31141 gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga
    31201 atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc
    31261 gagattgcct cctacttggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc
    31321 aatgtgcacc gcattgaaga accacagtgg gaaaactacg accgtgtggt cattggtgct
    31381 tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa gcatgcgacg
    31441 cggctgaatt cgatgccgag cgccttttac tccgtaaacc tggtggcgcg taaaccggag
    31501 aagcgcactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt
    31561 cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac
    31621 cgttttatga tcaagctaat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa
    31681 gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc
    31741 gacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcgga aaattatttt
    31801 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
    31861 acggcaaaca cgccgccggg tcagcggggt tctcctgaga attccg
//
LOCUS       DAPHJE010000046        31801 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPHJE010000046 DAPHJE010000000
VERSION     DAPHJE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.046"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(166..1107)
                     /gene="fabY"
                     /locus_tag="RH222_002156"
                     /gene_synonym="yiiD"
     CDS             complement(166..1107)
                     /gene="fabY"
                     /locus_tag="RH222_002156"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV3797172.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVDFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1152..1589)
                     /gene="dtd"
                     /locus_tag="RH222_002157"
     CDS             complement(1152..1589)
                     /gene="dtd"
                     /locus_tag="RH222_002157"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV3797173.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1586..2458)
                     /locus_tag="RH222_002158"
     CDS             complement(1586..2458)
                     /locus_tag="RH222_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV3797174.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2452..3051)
                     /gene="yihX"
                     /locus_tag="RH222_002159"
     CDS             complement(2452..3051)
                     /gene="yihX"
                     /locus_tag="RH222_002159"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV3797175.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3150..3935)
                     /locus_tag="RH222_002160"
     CDS             complement(3150..3935)
                     /locus_tag="RH222_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3797176.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3969..4865)
                     /gene="yihV"
                     /locus_tag="RH222_002161"
     CDS             complement(3969..4865)
                     /gene="yihV"
                     /locus_tag="RH222_002161"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDV3797177.1"
                     /translation="MIRVACVGITVMDRIYYVKGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFRVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5033..5929
                     /gene="yihU"
                     /locus_tag="RH222_002162"
     CDS             5033..5929
                     /gene="yihU"
                     /locus_tag="RH222_002162"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV3797178.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5953..6831
                     /gene="yihT"
                     /locus_tag="RH222_002163"
     CDS             5953..6831
                     /gene="yihT"
                     /locus_tag="RH222_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDV3797179.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6848..8089
                     /gene="yihS"
                     /locus_tag="RH222_002164"
     CDS             6848..8089
                     /gene="yihS"
                     /locus_tag="RH222_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDV3797180.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8227..9129
                     /locus_tag="RH222_002165"
     CDS             8227..9129
                     /locus_tag="RH222_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDV3797181.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASLNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9328..11364
                     /gene="yihQ"
                     /locus_tag="RH222_002166"
     CDS             9328..11364
                     /gene="yihQ"
                     /locus_tag="RH222_002166"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDV3797182.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAAALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            11410..12795
                     /locus_tag="RH222_002167"
     CDS             11410..12795
                     /locus_tag="RH222_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3797183.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            12838..14241
                     /locus_tag="RH222_002168"
     CDS             12838..14241
                     /locus_tag="RH222_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3797184.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFGNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            14309..15001
                     /gene="ompL"
                     /locus_tag="RH222_002169"
     CDS             14309..15001
                     /gene="ompL"
                     /locus_tag="RH222_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDV3797185.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15092..16357)
                     /locus_tag="RH222_002170"
     CDS             complement(15092..16357)
                     /locus_tag="RH222_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3797186.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16459..17439)
                     /locus_tag="RH222_002171"
     CDS             complement(16459..17439)
                     /locus_tag="RH222_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV3797187.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKAHRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17447..18157)
                     /locus_tag="RH222_002172"
     CDS             complement(17447..18157)
                     /locus_tag="RH222_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV3797188.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18374..20197)
                     /gene="typA"
                     /locus_tag="RH222_002173"
     CDS             complement(18374..20197)
                     /gene="typA"
                     /locus_tag="RH222_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV3797189.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20570..21979
                     /gene="glnA"
                     /locus_tag="RH222_002174"
     CDS             20570..21979
                     /gene="glnA"
                     /locus_tag="RH222_002174"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV3797190.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22298..23347
                     /gene="glnL"
                     /locus_tag="RH222_002175"
     CDS             22298..23347
                     /gene="glnL"
                     /locus_tag="RH222_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV3797191.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23359..24768
                     /gene="glnG"
                     /locus_tag="RH222_002176"
     CDS             23359..24768
                     /gene="glnG"
                     /locus_tag="RH222_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV3797192.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24880..24990
                     /locus_tag="RH222_002177"
     CDS             24880..24990
                     /locus_tag="RH222_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV3797193.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25180..26553)
                     /gene="hemN"
                     /locus_tag="RH222_002178"
     CDS             complement(25180..26553)
                     /gene="hemN"
                     /locus_tag="RH222_002178"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV3797194.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26742..27251)
                     /gene="yihI"
                     /locus_tag="RH222_002179"
     CDS             complement(26742..27251)
                     /gene="yihI"
                     /locus_tag="RH222_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV3797195.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVIEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            27833..28465
                     /locus_tag="RH222_002180"
     CDS             27833..28465
                     /locus_tag="RH222_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV3797196.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28847..31633)
                     /gene="polA"
                     /locus_tag="RH222_002181"
     CDS             complement(28847..31633)
                     /gene="polA"
                     /locus_tag="RH222_002181"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV3797197.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7726 a         8228 c         7878 g         7969 t
ORIGIN      
        1 acaaataata tttaaatgct cctgaacagt gatgtagcaa gacacgtatg gagccaacga
       61 agaataaaaa tttatagagt tcttattcaa tataaccgga atagtcaggg gctgatgtaa
      121 atgaccggaa tgagtaagag aggcagcacc gcccctctcc atcagctact cttcttcgtt
      181 cccgccctct tcatacgggc caaatggctt cgcgggcaga acgatatacg tgccttcaaa
      241 cactgcaccc ggcgtctcgt cgccaaagat ttcaacttgc atctgcaccc gtgcttttcg
      301 tccgcgcgcc agacggtcga gatcgccgct taaggcacca aggtcggcta ccgcatgagg
      361 tttaccgcta atcggcttgc tgtagcggat atgcgcatcc gccagaataa tcgttccgcc
      421 gagatggcgt tcgcgcagca tcagccagat aagcccccaa ccggtgagcg ttgccagtga
      481 gaataaactc ccggcaaaca gcgtatggtg cggattctga ttaccggttt ccggcatggt
      541 ggtgataaat ttttgcccgg tatattgttg aatacgcacg cccatttttt cactaagcgg
      601 gatatgttcg taccacgcct gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc
      661 atccagagtg gcaacgggtt taatcatcaa aaaatggcga atcggcgtgg tagttggggt
      721 ggtgatctcc ccctgattaa tgaatcccag cttggcgaaa aaatccaccg cgtcttcacg
      781 ggcgctacag gtcacgcgct ttacgccttc ctgacgcgcc accgactcca gggtcatcgc
      841 catcagcgtg cctaaccctt tgtcctgcac gtccgggtga acggccataa agcgaatgga
      901 cgcttcattg tcggcattaa tatacaatcg gcctaccgcc accagattac cctgctcgtc
      961 gacgaccatc tggtgatgcg ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg
     1021 atgcaggggc ttacgcaaca tttcccagcg aaactgatag taacgctcta attcttcttc
     1081 tgtttgtgga acccgaaggt gatacatagc gatactctct cttgtttccc gtgacaaccc
     1141 tggaagctgg ctcatacctg caaccagaat gtcacggggc catcattgac cagcgatacc
     1201 tgcatatccg cagcgaagcg tcctgtttgc gtgttcatct cttgctgacg gcagcgttcg
     1261 acgaaatagt catataacgc ctctgcgcga tccggtgatg cacctttgga gaaacttggg
     1321 cgcatccccc gttcggtatc tgcggcgagg gtaaactggg aaaccaccag cacactgccg
     1381 cccgcctgtt gcacgttgag gttcatcttg ccttcggcat cgctaaagat gcggtagccg
     1441 agcacacgct cgcacagacg gtttgctttc tgttcgtcgt catccttttc gacacccaat
     1501 aacaccaaaa gtcccgcgcc aatttcgccc gtcacttctc cctccacggt gacgctggca
     1561 cgggttacgc gttgaattaa tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt
     1621 gttttagttt gcggtattcc ccgagagtga cagtaatttc cgcgccaagc aagacgatac
     1681 accatgtcca gtagacccaa acaaagagaa tggggatcac cgccaacacg ccgtaaatga
     1741 gctgatatga cggaaacatg gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca
     1801 ggagcgcggc gacaaacgcg ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa
     1861 caatgctgta cagcaaccag aaggagatcc acgacaacag cagcggaaaa atacgcagca
     1921 cgttatcgat gacagtattg agatcgctcg cccagcgcag ggagagcaaa taggaactga
     1981 tcgccagact ggcccctgcc agcagcggcc ccagcgttaa aatcatccag tacacggcga
     2041 acgagtaaat ttttggtcgc gctcgtttac tgcgccagat ggtattcaac gcgctatcga
     2101 tggagtacat cagcaataac gcggtgacga tcagcccgca cgccccaacg gcggtcatct
     2161 tgttggaatt ggcaacaaat tgttcgatat accgctgaat aacatcgcca gtagcaggca
     2221 gaaagttggc aaaaataaag tgacgtaact gaatgctgac gtcggaaaac atgggaaaag
     2281 cggcgaaaag cgcaaaaaca acggcaacca gcggcactaa tgagagcaac gacacatagg
     2341 caaggttacc tgccagggtt gtcatgttgt cctcatcaat gcgttgccag agtagtttta
     2401 gccaggccca tagtggacgg gtacggtgcc tggctttgtc ctgaatggtt tttagcataa
     2461 caccttcgcg aaatagtccg ggatggtggt tttatctttc accagaatac tggtaatgcc
     2521 cagctgattg gctccttcta tattatcggc gttatcgtcg aaaaagaccg tatcgctggg
     2581 tgaaaaacct tccgcctgca aaacatgctg gtaaattcgt gcttcaggtt tgcgcatccc
     2641 cagatcttgc gacagataga tatggtcagc agcatcacga atttccgggt attcttccgg
     2701 ccagaaggtg gtatgcaggc ggttggtatt ggaaagcacc accacgcgat gcccctgctc
     2761 acgcagttta tgcatgatgg cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca
     2821 gccgtgagag aactgctcgt agcttagcgg tagagccatc tcatgacaca gcgcctctgc
     2881 gaacgcttcg tcgctaattt ccccacgctc atgctgatga aacgcctccc ccatatgaaa
     2941 actcttctta agcgatgcca gcggaatacg cgttaaatcg ctccaggctc ccagcacacg
     3001 gttaaagtcg atatcgacaa tcacattacc taaatcaaag atatagagca tttttgcctc
     3061 ctttcgcgcc acaagaaatt aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg
     3121 tatgaagcca ggttaacggt cacaaaaatc tacgcgtctt cctggggaag aatgatttca
     3181 atttggctgt cttgtaagcg tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc
     3241 gcagtgacct gtgccacgtt accaatttca accgccgccg aagcatgata cttagtgtga
     3301 tcggcgacca gcagaatatt tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg
     3361 tttacatcaa actccatcaa cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga
     3421 taatcagcac gaaaatcagc cacaaaggac gccgctgaag ggccaatgat cccgctatta
     3481 tgagagcgca acgtaccgcc gggcaccatc acttcaaagc gcgggttgtg gtaaagaata
     3541 tgcgccacac gcaggctgtt ggtgattatc cgcaaatgat tatggttaag taacgcccgg
     3601 gcaacatgct caacagtcgt accaatggtg ataaatattg ttgaaccatc aggaatatag
     3661 tctgccaccg cttcggcaat cgcttttttt tcctcggttt gcgaaacctc acgctgctcg
     3721 aacgccgtat taacgacgct ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg
     3781 ccttgctcgc ttaatttacg aatatcccgg cggaccgtct gcgtggagac atccagcaga
     3841 tttgccagct catcaatatt catataccca cgctcggcga tcagcatgag gagttggtcg
     3901 tgccgcgggt taccggttag ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc
     3961 ctggcatttt atacaaaaag tgacaaaaaa gatcgggttt gatcacagtc agggatcccg
     4021 gctcgtccac cgggacgtgt gcattttaac gccgctacac cgctggcgaa gcggactgac
     4081 tccgctaaat ccccacttgt tgccagcgcc accgccaaag cgccgtgaaa aacatcaccc
     4141 gcacctgtgg tatctaccac atcaactctg aaggccggtt gatgctgacg cccaccattt
     4201 tccagccagt cgcagcctgc gctaccctgg gtcacataga catgaccatt tgtgagcgtt
     4261 tgtgcctgtt ttagcgcact ggccatctct ttcacgcccg ttaagcgcgc cagacccggt
     4321 tctgaaaagg ccgcgtgatc gcttaatgcc accagctcac tgatatcctg cggcgtaatg
     4381 tccccgtcca gaacggtcat cacacccgcc tgacgggcca gggtgaaggc ttttttagcg
     4441 ccgtcgtgcc agcgtacatc tgccagcaca acatcccact gagagaaatc aatttcctcc
     4501 aaccactctg cgtcaggcag cagatccggg ctggggtagt taatgattat ccgctcgcct
     4561 ttggtatcca ccatgatggc ggattgcgaa gatttcgcct ggttataccg tttggtgtaa
     4621 cgggtgttaa cgccccagga ttccagctct gccagcaggc tgttgcccgt gtcgtcatca
     4681 cctacgcgac caataaaatc gacctgcgcc cccagccttg ccgccgcaac cgctgccgtc
     4741 gccgctggcc cgccaccaac ttccgtataa tttctcgcca cgtatttacc gctctccgtc
     4801 ggtaaccctt tcacgtaata gatgcgatcc atcacggtta tacctacaca agcaacacga
     4861 atcatggtca ttccttaaac attttgggat gcgttcattt taatttcacc aattgtttaa
     4921 aaagtgacgg atgtcaattt tttgacgata aattacaaat acgatcaaaa acagacaagc
     4981 aaagcatgtc ataaatgaca aaaagtgaca tcactgtatt caggagaggg ttatggcagc
     5041 aatcgcgttt atcggtttag gacaaatggg ttcgccaatg gcgagcaatt tattgcagca
     5101 agggcatcaa cttcgcgtct ttgatgtgaa cgccgaagct gtgcggcatc tggtagacaa
     5161 aggtgcgact cccgccgcca acccggcgca ggccgctaaa gatgccgaat ttatcattac
     5221 catgttgccg aatggcgatc tggtgcgcaa cgtgttgttc ggtgaaaacg gcgtttgcga
     5281 aggcttatct accgatgcgc tggtcattga tatgtccacc atccatccgc tgcaaaccga
     5341 taaattgatt gccgatatgc aagccaaagg cttcagcatg atggatgttc cggtaggccg
     5401 tacttctgca aatgccatta ccggtactct gttactgctg gctggcggca ccgctgaaca
     5461 agttgaacgt gccacaccga tcctgatggc gatgggcagt gagttgatca actctggcgg
     5521 tccgggcatg gggatccgcg ttaagctcat caacaactac atgagcatcg cgctcaatgc
     5581 gctttcggca gaagccgccg ttttgtgcga agccctgaat cttcccttcg atgttgccgt
     5641 caaagtgatg agcggtaccg ccgccggtaa aggtcacttc accacttcct ggccaaacaa
     5701 agtcctcagc ggagatcttt ctcccgcctt catgatcgat cttgcccata aggatcttgg
     5761 catcgccctt gatgtcgcca accagctgca tgtgccaatg ccgctggggg ccgcctcacg
     5821 ggaggtttat agccaggcgc gcgcagcggg tcgcggtcgc caggactggt ccgccattct
     5881 ggaacaggtc cgtgtcagtg ccgggatgac tgccaaagta aaaatgtaac gactggataa
     5941 aggaataaat gaatgaataa gtacaccatc aacgacatta cgcgcgcatc gggcggtttt
     6001 gccatgctgg cggtcgatca gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca
     6061 cccgctccgg tagccgatag cgttttaact gatttcaaag ttaacgctgc aaaggccctc
     6121 tcgccttatg cctcggcgat tctggtagat caacaattct gctaccgcca ggtggttgag
     6181 caaaacgcga ttgccaaaag ttgcgccatg attgtcgccg ccgatgagtt cattcctggc
     6241 aacggtattc cggtcgatag cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa
     6301 caagacggtg gcaaagcctt aaaactgctg gtgctgtggc gtagtgatga agatgcgcag
     6361 caacgtctgg atatggtgaa agagttcaac gaactgtgcc actcacacgg tctggtaagc
     6421 atcattgagc cagtcgtccg cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg
     6481 atcatcgatg ccgccaaaga gctgggcgac agtggcgctg acctctacaa agttgaaatg
     6541 ccgctttatg gcaaaggtcc gcaacaagag cttctctgtg cttcacaacg tctgaatgac
     6601 catatcaata tgccatgggt gatcctctct tccggtgtcg acgaaaaact gttcccgcgt
     6661 gccgtacgcg tggcaatgac ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg
     6721 gcatcggtcg tcggtttacc agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa
     6781 ttacaacaac ttggcgatat cgtcgacgaa atgatggcta aacgccgcta agatgaggat
     6841 aaaaggaatg aaatggttta acaccctaag ccacaaccgt tggctggaac aggaaaccga
     6901 ccgcatcttt gattttggta aaaattccgt agtgccgact ggttttggct ggttaggcaa
     6961 taaagggcaa atcaaagaag agatgggcac ccatctgtgg atcaccgctc gtatgttgca
     7021 cgtttattcc gttgctgcgg cgatgggtcg acctggcgct tactcgttgg ttgatcacgg
     7081 tatcaaagcc atgaacggcg cactgcgtga taaaaaatat ggcggctggt atgcctgcgt
     7141 gaatgacgag ggcgtggtgg atgcctccaa acagggctat caacacttct ttgctctgct
     7201 gggtgccgcc agcgccgtca caacgggtca cccggaagcg cgcaagctgc tcgattacac
     7261 cattgaaatt atcgagaaat atttctggag cgaagaagag cagatgtgcc tggaatcctg
     7321 ggacgaagcc ttcagcaaaa ccgaagagta ccgcggcggc aatgccaata tgcacgcggt
     7381 ggaagctttc ttgattgttt atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat
     7441 tcgcgtggct tccgtgatta tccacgacgt cgccagaaat aatcattatc gcgttaacga
     7501 acacttcgat acccagtgga atccgctgcc ggattacaac aaagataacc cggcgcatcg
     7561 cttccgcgcg tttggaggta caccaggcca ctggatcgaa tggggccgtt taatgctgca
     7621 catccatgct gccctggaag cccgttgcga acaaccacca gcatggctgc tagaagatgc
     7681 caaaggtctg tttaacgcca ccgtgcgcga tgcctgggca cccgatggcg cggacgggat
     7741 tgtttatacc gttgactggg aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat
     7801 cgtcgaagca atgggtacgg cctacgcgct ctacaccgtc actggcgatc gccagtatga
     7861 aacctggtat caaacatggt gggagtactg cattaagtac ctgatggact atgaaaatgg
     7921 ttcctggtgg caggagctgg atgcggacaa taaggtcacc accaaagtct gggacggcaa
     7981 acaggatatt tatcacctgc tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg
     8041 catggctcca gcggttgcag cgggtctgct ggatattaat gcgaaataac tgatctgaaa
     8101 atagggtgcg ggttttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc
     8161 ggtgcagttt ttgggtttga catcagcctc acgggaggct gtatgtcgtt aattaaggtg
     8221 ccttgtatgc aaatcaccaa tatgcattgc agtggacaga ccgtttcact tgccgcgggg
     8281 gattatcacg cgaccattgt gaccgtcggt gctggtctgg ctgaattaac ctttcagggt
     8341 tgtcatttag tcatcccgca caaacccgaa gagatgccgc tggcgcatct gggtaaagtg
     8401 cttattccgt ggccgaaccg gatcgccaac ggctgttacc gctatcaggg gcaggaatat
     8461 cagttaccca ttaatgaaca tagttcaaaa gccgctattc acgggctgct tgcctggcga
     8521 gactggcaaa ttagtgaatt aaccgccacc agcgttaccc tgacagcttt tctgccgccc
     8581 agttacggct atccgtttat gctggcttca caagttgttt attcgctcaa cgcacacacg
     8641 ggtctttccg ttgagatcgc aagcctgaat atcggcactg tcgcagcgcc ttatggcgtg
     8701 ggtattcata cttatttaac ctgcaactta acatctgttg atgaatatct gttccagctt
     8761 ccggcaaacc aggtttatgc cgttgatgaa cacgtaaatc cgacaacgct tcaccacgtt
     8821 gatgagctgg atttaaattt cacgcaggcg aaaacaatag ccgcgacaaa aatcgaccac
     8881 acctttaaaa ccgctaatga tctgtgggaa ataaccatta cccatccaca acaggcgctt
     8941 tcggttagcc tttgttgcga tcaaccctgg gtacaaattt atagcggtga aaaattacag
     9001 cgtcagggac tggcagttga acctatgagt tgcccgccta acgcctttaa ttcaggtatc
     9061 gacttattat tactggaacc aggaaaaccg caccgcctgt tttttaacat ttacggacag
     9121 agaaaataac ttgtattgat tagcagtcaa aatcattgaa gaacatttca ctcgctaaag
     9181 gcaacattat gtatcttcaa tttattaagt taaaaacagc tcagcttctg taaagttttt
     9241 atatatcagt tttatgagaa tcattttaca taagcctgta ataacaggaa ggcaacaatc
     9301 tcgttctcaa ctctatggag attagttatg gatacgccac gtccacagtt attagatttt
     9361 caatttcatc agaataacga cagttttacc ctacattttc aacaacgtct tattttaacc
     9421 catagcaaag ataatccttg tttatggatt ggctcaggta tagcggatat cgatatgttc
     9481 cgcggtaatt tcagcattaa agataaacta caggagaaaa ttgcgcttac cgacgccatc
     9541 gtcagccagt caccggatgg ttggttaatt catttcagcc gtggttctga cattagcgcc
     9601 acgctgaata tctctgccga cgatcagggc cgtttattgc tggaactaca aaacgacaac
     9661 cttaaccaca accgtatctg gctgcgcctt gccgctcaac cagaggacca tatctacggc
     9721 tgcggcgaac agttttccta cttcgatctg cgtggcaaac cgttcccgct atggaccagt
     9781 gaacaaggcg ttggtcgcaa caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa
     9841 aatgcgggcg gcgactatta ctggactttc ttcccacagc ctacgtttgt cagcacgcag
     9901 aagtattact gccatgttga taacagttgc tatatgaact tcgactttag tgccccggaa
     9961 taccatgaac tggcgctgtg ggaagacaaa gcaacgctgc gttttgaatg tgctgacaca
    10021 tacatttccc tgctggaaaa attaaccgcc ctgctgggac gccagccaga actgcccgac
    10081 tggatttatg acggagtaac gctcggcatt cagggcggga cggaagtgtg ccagaagaaa
    10141 ctggacacca tgcgtaacgc gggcgtgaag gtcaacggca tctgggcgca ggactggtcc
    10201 ggtattcgta tgacctcttt tggcaaacgc gtgatgtgga actggaagtg gaacagcgaa
    10261 aactacccgc aactggattc acgcattaag cagtggaatc aggagggcgt gcagttcctg
    10321 gcctatatca acccgtatgt tgccagcgat aaagatctct gcgaagaagc ggcacaacac
    10381 ggctatctgg caaaagatgc ctctggcggt gactatctgg tggagtttgg cgagttttac
    10441 ggcggcgttg tcgatctcac taatccagaa gcctacgcct ggttcaagga agtgatcaaa
    10501 aagaacatga ttgaactcgg ctgcggcggc tggatggctg acttcggcga gtatctgccc
    10561 accgacactt acctgcataa cggcatcagc gccgaaatta tgcataacgc ctggcccgcg
    10621 ctgtgggcga aatgtaacta cgaagccctt gaagaaacgg gcaagctcgg cgagatcctt
    10681 ttctttatgc gcgccggttc taccggtagc cagaaatact ccaccatgat gtgggcgggc
    10741 gaccagaacg tcgactggag tctcgacgat ggcctggcgt cggttgtccc ggcggcgctg
    10801 tcgctggcaa tgaccggaca tggcctgcac cacagcgaca ttggcggtta caccaccctg
    10861 tttgagatga agcgcagcaa agagctgctg ctgcgctggt gcgatttcag cgccttcacg
    10921 ccgatgatgc gcacccacga aggtaaccgt cctggcgaca actggcagtt tgacggcgac
    10981 gcagaaacta tcgcccattt tgcccgtatg acctccgtct tcaccaccct gaaaccttac
    11041 ctgaaagagg ctgccgcgct gaatgcgaag tccggcctgc cggttatgcg cccgctgttc
    11101 ctgcattacg aagacgatgc gcacacttac accctgaaat atcagtacct gttaggtcgc
    11161 gacattctgg tcgctccggt gcatgaagaa ggccgtagcg actggacgct ctatctgccg
    11221 gaggataact gggtccacgc ctggacgggt gaagcgttcc ggggcgggga agttaccgtt
    11281 aatgcgccca tcggcaagcc gccggtcttt tatcgcgccg atagcgaatg ggcggcactg
    11341 ttcgcgtcgt taaaaagcat ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg
    11401 agaacatcga tgagtcacat cacaacggaa gatccagcaa ctttacgcct gccctttaaa
    11461 gagaaactct cttacggtat tggcgacctg gcctctaaca tcctgctgga tatcggtaca
    11521 ctttatcttt tgaagtttta taccgacgtt ctggggctgc caggcaccta tggcggcatt
    11581 atctttttga tttcaaaatt ctttactgcg tttaccgata tgggtaccgg cattatgttg
    11641 gattcccgac gcaagatcgg tccaaaaggt aagttccgtc cttttattct gtatgcgtca
    11701 ttcccggtca ccttactggc gatcgccaac tttgtcggca caccgtttga tgtcaccggt
    11761 aaaacggtga tggccactat tctgtttatg ctttacggac tgtttttcag catgatgaac
    11821 tgctcctacg gcgcaatggt tcccgctatc accaaaaacc ccaacgaacg cgcctcactg
    11881 gcggcatggc gtcagggcgg cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg
    11941 ccagtgatga atcttatcga gggtaatcag caacttggct atatcttcgc cgccacgctg
    12001 ttttcactgt tcggcctgct gtttatgtgg atctgctact caggcgtgaa agagcgttat
    12061 gtcgaaaccc aaccaaccaa tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca
    12121 attgccggta accgcccact gttcattctg tgtattgcca acctctgcac cttaggggcg
    12181 tttaacgtca agctcgccat tcaggtctat tacacccagt acgtacttaa cgatcccatc
    12241 ctgttgtcgt atatgggatt tttcagcatg ggctgtattt tcatcggcgt gttcctgatg
    12301 cctggcgcag tcagacgttt tggtaagaag aaggtctata tcggcggcct gctgatttgg
    12361 gtgctgggcg atctgctcaa ctatttcttc ggcggcggct cggtcagctt tgtggcgttc
    12421 tcctgcctgg cgttcttcgg ctcagcgttt gttaacagcc tgaactgggc gctggtttcc
    12481 gacaccgtcg agtacggcga gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc
    12541 ggcttcacct tctttcgcaa agtgtctcag gcgctggctg gtttcttccc cggctggatg
    12601 ctgacgcaaa ttggctatgt gccgaacgtc gcacaggctg accacaccat tgaagggtta
    12661 cgccagttga tcttcatcta cccaagcgca ctggcggtag tcaccattgt ggcgatgggt
    12721 tgcttctaca gcctgaacga gaagatgtat gtccgcattg tggaagagat agaagcacgt
    12781 aaacgcacgg cgtaataata ataaacgacg ccctgcgggg cgttataagg agtgattatg
    12841 tctgaccata atccactgac attaaaactg aatctgcggg aaaaaatcgc ctatggtatg
    12901 ggcgacgtcg gttcgaattt aatgctctgc atcggtactc tgtatctcct caaattttat
    12961 accgatgaat tagggatgcc tgcttactat ggcgggatta tctttctggt ggcgaagttt
    13021 tttaccgcgt ttaccgatat gctcaccgga tttttactcg actcgcggaa aaatattggg
    13081 cctaaaggca aatttcgtcc ctttatttta tatgcagcag ttccggcggc gttaattgca
    13141 acgctccagt ttatcgccac caccttttgt ttgccggtta aaacgacgat tgccaccgcg
    13201 ctgtttatga tgtttggact ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc
    13261 ccggcaatta ccaaaaaccc gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt
    13321 gcaaccatag ggctattgat ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct
    13381 gactcaaccg tcggttatgc ctgtgcggca cttatgttct ccattggcgg ctttattttt
    13441 atgatgctgt gctacagagg cgtcaaagag cattatgtgg acacagcgcc aaccggacat
    13501 aaagccagta ttctcaaatc tttttgcgcg atatttggta atccaccatt gctggtttta
    13561 tgcattgcta acctgtgtac tctggcggca tttaatatca aactggcgat tcaggtctat
    13621 tacacccagt atgtgctgaa tgatattaat ttattgtcgt ggatgggatt cttcagtatg
    13681 ggatgcatcc tcgtcggcgt attactggtg ccagtaactg taaaatgttt tggtaaaaaa
    13741 caggtttatt tagctggcat ggtgctgtgg gcggtgggtg atatactgaa ttatttctgg
    13801 ggaagtaact ctttcacttt cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt
    13861 gtaaacagcc tgaactgggc actggtacca gataccgtag attacggtga atggaaaacc
    13921 ggtattcgtg ccgaaggttc tgtttatacc ggttatacct tctttcgtaa aatttctgcc
    13981 gcacttgctg gcttcttgcc aggcattatg ctgacgcaaa ttggttatgt tcccaacatc
    14041 gcgcaaagcg atgcgacatt acagggtttg cgtcagctca tttttatctg gccttgtgca
    14101 ctggcaatta ttgctgcatt gacgatggga ttcttttaca cactcaatga aaaacggttt
    14161 gcattgatta ttgaggaaat caaccaacgc aaaaataaag agatggcgac agaagaaaaa
    14221 acggcttccg taacgttata aatgtcaata gccgctattt ccatcctggt gggtggcggc
    14281 ctccctacgt ttaaaaaatg gacttattat gaaaaagatt aatgcaataa ttttattatc
    14341 ttctctgacc tctgcctcgg tatttgctgg cgcatacgtc gaaaacaggg aggcatataa
    14401 tcttgcctcc gatcagggcg aggttatgct acgtgtgggt tataacttcg atatgggcgc
    14461 gggtattatg ttaaccaata cctacacctt tcagcgagaa gatgaactaa aacatggcta
    14521 taatgaaatt gaaggttggt atcctttatt caaaccaacc gataaattaa ccatccagcc
    14581 cggtggctta attaatgata agagtatcgg ttcgggtggt gcagtgtatc tggacgtcaa
    14641 ctataaattt gtaccatggt ttaatctgac agtacgaaat cgctataacc ataataacta
    14701 tagttcaaca gatttgagcg gggaactgga taataatgac acctatgaaa ttggcaccta
    14761 ctggaatttt aaaatcaccg ataaattttc ctatacattt gagccacatt atttcatacg
    14821 cgtaaatgac tttaatagca gcaatggaaa agatcatcac tgggaaatca ctaatacctt
    14881 ccgttatcgc attaatgaac actggctgcc ttatttcgaa ttacgttggt tagatcgtaa
    14941 tgtcgaaccg taccaccgcg agcaaaacca gattcgtatc gggacgaagt atttcttctg
    15001 atgtgaaact gccggatgcg gcgtaaacgc cttatccggc ctacggttga tacggttttc
    15061 gttggccgga ttaagcacat ccggccatta atcaagcttc agccgccagc atctctttct
    15121 tacgttggct aattcgctta accagcatta cggaaaccac cgcaccacag aatgcgaagc
    15181 aggccataat gccaaacacg attttgtagc caataatccc cggatttaaa tccagaatgt
    15241 agccatacag actgaagcag aacatcgccg gagcgtaacc aataaagcta cccaacgcca
    15301 tcgccgcgcc tgttttattt tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg
    15361 ctcgctgtgt aaagactatc gcgccaaagc ccagcgtaca tgccatccct aaatagaccg
    15421 gcatactttc gtgcggcagc ataatcaaca gtacgagcgc agcggtactg atgataaagg
    15481 tgtagcatag atatttactc ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg
    15541 gtccaccaat cattttcaga cagtattggt taatgatgcc gtaagccccc accagcgcaa
    15601 cgggcaatag atagatgttt ttcaggaatg gaatgaagaa tgtcaggcca cagtaaaccg
    15661 cgtaaacaaa gaagacgtta aaagcgataa gccagatggt tttgtctctc agcaccgagg
    15721 tcatactggt gttttgcgat gctccgtctt cttttttcac ctcaacggac ggtgcctctt
    15781 ctttgtcatt caggacaaag aaaataataa tgcctacggc aatcacaata agggaataga
    15841 accagatgcc tgctttaaaa cctaataagc cactgccaaa ccaggtaaat actgccaacg
    15901 cagaaaatgc caccacggta tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc
    15961 gaccttgttg ttcgctattg cccaatcgac ttaccgactt gagcaagacc ggccagttca
    16021 tcatgtcgca agtaacgcca aatagcgccc agacaaagag gatgccccaa taccccggca
    16081 ttgtcgtcaa ataaacaccc agtaatcctg tcgcaatgag tgagaatgac atggtgtatc
    16141 tgcgcggtag tttatcggca aaataaatag acagaaaaaa gcccactgtg gtgacaaatg
    16201 agtttaccga catagcatta ccaatttgac cattggtcaa atggaaatat tcctgcatcg
    16261 ggatataaaa cgcatctttc agcgacggta atttataaat tgtaccgcca cacagtgtta
    16321 atagactaaa taacgcccat ttctttttcg tgagcataac atcccctaag cgatttttaa
    16381 aatcactgtt ttaaaggcaa taagataccg accattcata tagaatggtt ttattaactg
    16441 cgtatgagtt aaaacttatt agtggttcag aagatggttt gcctcttgtt tgatttgttg
    16501 tagtacgtta cgcacatgat ttatctgatt aattgcatct tctttttctt tacgtaaccg
    16561 cgcatccagt aaatgatttt ctggtagggg tgtttcactc atctggcgat aagggatcca
    16621 cggattatca tcttcgtctt caaagcggaa cgccggcgca tagtaatcaa cctcttcttc
    16681 caggccatat gccgtcacct gcggctcatc atcaccaagg cagataaggt gcgttaataa
    16741 cgctttgaac ggcatatcac cctgacctga aatacaggct ttatgaccca ggccaccctg
    16801 ttctttaacg atcaaggcat ctttaatatg gacctgggta atatgcggtg ccatcgtttt
    16861 taaggcgtcg atgggatgct catttgcatt gatcatgttc gcaaaatcaa acagtaagga
    16921 aagagactcc atctcacttt ctttgaccag cgacaccaac tcatgacttt ttaaatcttc
    16981 atgctgctcg atcgtaaaag tcaggccgct gtcctgatac gtttcccgta cataggcaat
    17041 gtcgttagcg ataatcgata atacgtcgcg caaattacct tcataacgtg gataaaaacg
    17101 tacggacgat gccccagttt tcaacgcgat ggcgacggct tcgtcaatag atgccttatc
    17161 tgaggcgctg gtttcaatat gaatatcaag gttcagacgg tgggctttat ccccaaaggc
    17221 agagagttct ttatcgtcca tattaccaag agaaaaacgc tcgccatcca gaacatggat
    17281 tttgacgcca cgtagattat tttcacttgc gatatcaagt aaatcagcag gcaacactct
    17341 ttccagccgc atatttaaat gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac
    17401 acgttgtaga atctttcttg cgttatttat tgtcaccata aaacacctac agattaatga
    17461 tgaggtctac ggcctcaaat cgccaatatt caatatctat ctggacaata ttgccttgct
    17521 ggtcggcgcg atgcttttca attaagatcg ctggcatacc cgcagaaccg ccaagatact
    17581 tgctgatatc gcccggcatt ctgacgggtt tgaaaatcaa tttttttact accgtctctt
    17641 tttggtagca cttttcccag actgcagaaa atgagtggtt ttcaagttgt tcaataaaac
    17701 ccggagcaac ttcaggatta atatatgttt cgtgatagaa aactttatgt ccttccagcg
    17761 ccccccagcc ggtgatgcga tagagttcag ttgatggtgt cactgcaatc agtggcgcga
    17821 gcgtcttggg aatatcgctg gtacggtttt tctcggtaaa cccccaggaa ggctctcgtc
    17881 cttgctcaat tgcggcccgc tgaaagctcg ccgacaactc cggactgtaa ttaaatcgcg
    17941 gctgggtcac gaaccacccc ttacgatcct tacggaagat tttggattct gcctcgaggt
    18001 tcaacaacgc ctggcgcagc gtcatacgtt taatgcccag cagttctccc agctctcttt
    18061 ctgaagggag ttttccaccc ggcgtggtga tgccatcttt caaccaccga tccagtttct
    18121 cttttgcatt ttctacggtg gattgattct ccgccattgt ttaatccttt tggtttaaac
    18181 cagatgaagc atcttacgct tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc
    18241 agataattgt gacaaagctc tctattgacg taatcccaga cttataaggg agatactcgc
    18301 attggtgtgc gctcacgaca ggtaaaaaaa aacctgccag cgatggcagg ttttttataa
    18361 ctaaaaaacg aaattaatcg tctttcggtg cgcggttggc gcgacgacga tcgttttcag
    18421 tcaggtgacg tttacgaata cggatagagg tcggagtcac ttctaccagt tcgtcatcat
    18481 cgatgaactc cagagcttgt tccagagtca tgcggatagg cggaaccaga acaacggctt
    18541 cgtcagtacc ggaagcacgc atgttggtca gtttcttacc agtcaggcag tttaccgtca
    18601 ggtcgttaga gcggctgtga ataccgataa tctgaccttc gtaaacttct gcaccgtgac
    18661 cgaggaacag cttaccgcga tcctgcagac cgaacagcgc gaacgcgacc gctttaccct
    18721 gaccgttaga gatcagtacg ccgttctgac gctgacccac ttcacccgga cgtacgtcgt
    18781 cgtagtggct gaaggtggag tacagcagac cagtaccgga agtcatggtc atgaactcag
    18841 aacggaagcc aatcagacca cggcttggga tcacgtagtc gagacgtacg cggcctttac
    18901 cgtctggatt catgtttttc aggtcgcctt tacgttcgcc cagcgcctgc attacagaac
    18961 cctgatgctg ttcttcaacg tccagagtca cgttttcata cggctcttgt ttacgaccgt
    19021 cgatttcacg gaagataact ttcggacggg ataccgccag ttcgaaacct tcacgacgca
    19081 tgttttcgat cagaacagac aggtgcagtt cgccacgacc agaaacgcgg aacgcatcgg
    19141 cgtcttcggt ttcttctacg cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat
    19201 ccaggatctg acgagacgtt acgaacttac cttctttacc gcagaacggc gaggtgttaa
    19261 cgcagaagaa catagaaacg gtcggctcat caacggagag tgccggcagc gcttcaacgt
    19321 tttgcgtgtc gcaaacggtg tcagaaatgt tcagttcgcc aaggcccgtg atcgcaacga
    19381 tatcgccagc ttccgccaga tcggtttcga tacgttccag accgaggtgg cccagcactt
    19441 taccgacttt cgcgttgcgg gttttgcctt cgctatcgat gatagtgacc tgctggttcg
    19501 gcttcacttt accgcgcttg atgcggccaa tgccgataac gccaacatag ctgttgtaat
    19561 cgagctgaga aatctgcatc tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt
    19621 gatcaacaat cgcctgatac agcggggtca tgtcttccgc catatcttcg tggtccagac
    19681 ccgcgatacc gttcagcgca gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg
    19741 cgtcgaggtt aacgaacaga tcgaatacct gatccacaac ccaatcagga cgcgcgccag
    19801 ggcggtcaac tttgttgata acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg
    19861 ttacgaagcg cgtttgcggc atcgggccgt caaatgcgtc aaccaccagc agcactgagt
    19921 ctaccatgga cattacacgt tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa
    19981 cgatgttgat acggtaatca ttccatttga tagcggtgtt tttcgcgagg atggtaatcc
    20041 cacgctcttt ctccaaatcg ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt
    20101 cgaacgtacc ggattgttgg agcagcttgt ctaccagggt ggttttacca tggtctacgt
    20161 gcgcgatgat ggcgatatta cgcaattttt cgatcacaac tttgcctcag gcattagaaa
    20221 tagcgcgtta ttgtacacgg attaatcgca ctacaaaaca ggatcacaaa catcctccgc
    20281 aaacaagtat tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa gggttatcca
    20341 aaggtcattg caccaacatg gtgcttaatg tttccattga agcactatat tggtgcaaca
    20401 ttcacatcgt ggtgcagccc ttttgcacga tggtgcgcat gataacgcct tttaggggca
    20461 atttaaaagt tggcacagat ttcgctttat cttttttacg gcgacacggc caaaataatt
    20521 gcagatttcg ttaccacgac gaccatgacc aatccaggag agttaaagta tgtccgctga
    20581 acacgtactg acgatgctga acgagcacga agtgaagttt gttgatttgc gcttcaccga
    20641 tactaaaggt aaagaacagc acgtcactat ccctgctcat caggtgaatg ctgaattctt
    20701 cgaagaaggc aaaatgtttg acggctcctc gattggcggc tggaaaggca ttaacgagtc
    20761 cgacatggtg ctgatgccag acgcatccac cgcagtgatt gacccgttct tcgccgactc
    20821 caccctgatt atccgttgcg acatccttga acctggcacc ctgcaaggct atgaccgtga
    20881 cccgcgctcc attgcgaagc gcgccgaaga ttacctgcgt tccactggca ttgccgacac
    20941 cgtactgttc gggccagaac ctgaattctt cctgttcgat gacatccgtt tcggatcatc
    21001 tatctccggt tctcacgttg ctatcgacga tatcgaaggc gcatggaact cctccaccca
    21061 atatgaaggt ggtaacaaag gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc
    21121 gccggtagac tctgctcagg atattcgttc tgaaatgtgt ctggtgatgg aacagatggg
    21181 cctggtggtt gaagcccatc accacgaagt agcgactgct ggtcagaacg aagtggctac
    21241 ccgcttcaat accatgacca aaaaagctga cgaaattcag atctacaaat atgttgtgca
    21301 caacgtagcg caccgcttcg gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga
    21361 taacggctcc ggtatgcact gccacatgtc tctgtctaaa aacggcgtta acctgttcgc
    21421 aggcgacaaa tacgcaggtc tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa
    21481 acacgctaaa gcgattaacg ccctggcaaa cccgaccacc aactcttata agcgtctggt
    21541 cccgggctat gaagcaccgg taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat
    21601 ccgtattccg gtggtttctt ctccgaaagc acgtcgtatc gaagtacgtt tcccggatcc
    21661 ggcagctaac ccgtacctgt gctttgctgc cctgctgatg gccggtcttg atggtatcaa
    21721 gaacaagatc catccgggcg aagccatgga caaaaacctg tatgacctgc cgccagaaga
    21781 agcgaaagag atcccacagg ttgcaggctc tctggaagaa gcactgaacg aactggatct
    21841 ggaccgcgag ttcctgaaag ccggtggcgt gttcactgac gaagcaattg atgcgtacat
    21901 cgctctgcgt cgcgaagaag atgaccgcgt gcgtatgact ccgcatccgg tagagtttga
    21961 gctgtactac agcgtctaat agttgaagtt gtactacccg gcgcaacaac gccggaggtt
    22021 caaaccaggc ccatctggcg taattgttgc agccagtttg aacacggaca gcgcgcagaa
    22081 cccggagcgt acactggtac gtgaggagtt cgagcactgc ccaggttcaa aatggcaaat
    22141 aaaatagcct gatgggactg gtttttagtt gccgtggaaa tttcagccca tctctgcatg
    22201 ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa aagctataat
    22261 gcactaaaat ggtgcaacct gttcaggaga ctgctttatg gcaacaggca cgcagcccga
    22321 tgctgggcag atcctcaact cgctgattaa cagtattttg ttaatcgatg acaacctggc
    22381 gatccattac gccaaccctg ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt
    22441 tggtacaccg ttaccggaac tgttgagcta cttctcatta aatatcgagc tgatgcaaga
    22501 aagtctggag gcggggcaag gttttaccga taacgaagtg acgctggtca tcgacgggcg
    22561 ctcgcatatc ctttctgtga cggcccagcg tatgccggac ggcatgatcc tgctggagat
    22621 ggctccgatg gataaccagc gccgcttaag tcaggaacag ctacagcacg cccagcaggt
    22681 tgctgcccgt gatttagtgc gcggcctggc acatgagatt aaaaatccgc ttggcggttt
    22741 acgtggcgcg gcgcagctac tcagcaaagc gttacctgac ccatcactac tcgaatatac
    22801 caaagtgatt atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc
    22861 gcagctgccc ggtacgcgcg ttaccgaaag tattcacaaa gtagctgaac gcgtggtaac
    22921 gctggtgtcg atggaactgc cggacaacgt gcggttgatt cgtgattacg atcccagcct
    22981 gccggaactg gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa
    23041 tgcgctacag gcgctggggc cggagggtgg tgaaatcatt ctgcgtaccc gcaccgcgtt
    23101 tcaactgacc ttacacggcg agcgctaccg gctggcggcg cggattgatg tggaagataa
    23161 cgggccgggc attccgcctc atttgcagga tacgctgttt tacccgatgg tcagcggccg
    23221 cgaaggtggc accgggcttg gcttatccat cgcccgtaat ttgattgatc agcattcagg
    23281 caaaattgaa tttaccagtt ggccaggtca taccgagttc tcggtttacc tgcctatcag
    23341 gaaataaagg tgacgtttat gcaacgaggg atagtctggg tagtcgatga cgatagttcc
    23401 atccgttggg tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac gacatttgag
    23461 aacggcgcgg aagtactgga ggcgctggcg agcaaaacgc cggatgtgct gctttcagat
    23521 atccgtatgc cgggaatgga cgggctggcg ctgctcaagc agattaaaca gcgccatcca
    23581 atgcttccgg tcatcattat gaccgcacat tccgatctgg atgctgccgt cagcgcctat
    23641 caacaagggg cgtttgatta tctgcccaaa ccgtttgata tcgacgaagc cgtcgcgctg
    23701 gttgagcgcg ccatcagtca ttaccaggaa cagcagcagc cgcgtaatat tcagcttaac
    23761 ggcccaacga ccgatatcat cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc
    23821 ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg caccggtaaa
    23881 gaactggtcg ctcatgccct gcatcgccac agtccgcgcg ccaaagcgcc gtttatcgcg
    23941 ctgaatatgg cagctatccc aaaagatttg atcgaatcag aactgtttgg ccacgagaaa
    24001 ggcgcgttta ctggcgcgaa taccattcgt caggggcgtt ttgaacaggc cgatggcggt
    24061 acattattcc tcgacgaaat tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc
    24121 gtgctggcag acggtcagtt ttaccgcgtt ggcggctatg cgccggtgaa agtggatgtg
    24181 cggattatcg ctgccactca ccagaatctc gaacagcgag tgcaggaagg taagttccgt
    24241 gaggatctgt tccaccgcct gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt
    24301 cgggaagata ttccccgtct ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc
    24361 gtagaagcga agttactgca tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca
    24421 ggcaacgtgc gccagctgga aaacacctgc cgctggctaa cggtgatggc cgccgggcag
    24481 gaagtgttga ttcaggattt gcccggcgaa ctgtttgaat caacggttgc ggagagtact
    24541 tcgcaaatgc aaccggacag ctgggcgacg cttcttgcgc agtgggcaga cagagcgctg
    24601 cgttccggtc atcaaaatct gctttccgaa gcgcagccag agctggagcg gacgttactg
    24661 acgaccgcgt tgcgacatac gcaggggcat aaacaggaag cggcgcggct actcggctgg
    24721 ggccgcaaca ccctgacgcg taagttaaaa gagctgggga tggagtgatt cacggcgtgt
    24781 gtgtaaagat tgattattga gcgcaaattg ctggtatttt acgctttact gttccgataa
    24841 gttcagtatg atcttgcccg gaaaacgggg agagtcatta tgctggaatc aataattaat
    24901 ctggtatcga gtggcgcagt tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg
    24961 tgtgccgcaa tgattgggct gtttagttga gtttcagatt gctgatgaag tgctctttgt
    25021 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aagcaatatt ttcaacaggt
    25081 tttgtaggcc tgataagcgt agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc
    25141 tttcgttttc tactttgtaa acgaagcgcc attcactatt taaatcaccc gtgagaactg
    25201 ctgcatccgc gctttctggc gcagataggt atcaaagcac atgcaaatgt tgcggatcag
    25261 caagcgacct ttcgccgtca cctgtattcc cttctcatcc acatccacca gcccatcttt
    25321 tgctaacggg gcgagcagct tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca
    25381 ctgtttctca ataggggcgt aatccagacg gaagttgcag atgagcgact taatcacatc
    25441 gcggcgaata cagtcatcac gcgttagcgc aataccacgc cacagcgcat tgccttgttc
    25501 atccacttgc tgatagtact gcttcaactc tttctggttc tgcgcgtagc agtcgccaat
    25561 catgctgatg gcggaaacgc ccatccccag cagatcggta tcgccctgag tggtgtagcc
    25621 ctggaagtta cgatgcagca cgccttcacg ctgggccacc gccagctcgt catccggacg
    25681 ggcaaagtga tccataccga taaactgata gcccgattgc gtcaggaagg cgatggtttc
    25741 ctgcaggata tcgagttttt gctgcggact cggcaggtca gcatctttga ttttgcgctg
    25801 agcagcaaaa atggtcggca gatgcgcgta gttaaagaca ctcagacgat cggggttcag
    25861 ctccgccaca cgtttcaggg taaaggcgaa actctccggc gtctgtttcg gcaggccgta
    25921 aatcaggtcg atgttggtgg aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa
    25981 gatgaactct tcatcctgct cgcggttaac cagacgttgc acttctttgt tgaagtcctg
    26041 cacgcccatg ctcaggcgat taaagccttc ggcgcgtaaa tgatcgagta catccagttc
    26101 gatttcccgc ggatcgactt cgatcgaaat ctccgcatcg gcattgaact ggaagttttc
    26161 gcgcagcagc ttcatcaggc ggctgatttg cgctttattc agatacgtcg gcgttccgcc
    26221 gccccagtgc aattggctga cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc
    26281 ttgctccagc gcgtccagat actgatcggc cttgtgctgc tggcgagtaa caatcttatt
    26341 gcaaccgcag aagtaacaaa gcttatggca gaacgggata tgtacgtaga gagataatgg
    26401 acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa
    26461 ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa tagttatatt tctggatcag
    26521 ggccagatcc cagtcgattt gctgtacaga catgctcact ccttccgatg gcgtctctgg
    26581 cggctacggc gtaccggcgg atgcccgttt cgggtcatca gccggttgcg cagccacttt
    26641 tgtcgccgcg acaaccgctg tagtttaacg aataaccaca ccagataaca tataaccgga
    26701 agggttatca gcagaacggg aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga
    26761 cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg
    26821 ccgagtttct gcatcaactc atcaatacga tccagtttag catccaccca ggattgctct
    26881 tcggcgctca gggtttcgcc tgcttccaga cgttccagca gcgcatccag acgctcatcc
    26941 gtttccagta attccaactc cgcctgcggt gaaagcatag gtttctcact cttcggtttg
    27001 tgctgtttgg tgactttttc aatcacgccc aatggaatag gggttttact gccaatacgt
    27061 ggatcttttg gtgcgttctg gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg
    27121 ctgcccggcg catgaccacg acgttttttc tgacgcttgc gatcacgagc ttcctgatcc
    27181 agctcctcgc gtgtttttcg acgcgctttt gcatgacctt tgctgcgtga gtttgaagat
    27241 gatggtttca taatggttcg tcttttagcc gttttattca gtatagattt gcggcggaat
    27301 ctaacagaaa gcaagcaaag aaaaaaggcg acagattact ctgtcgcctt ttttcctgac
    27361 tcataaccct taacgggtct tacaatcctt gcaggccgcc aacacaccct gtttttcctt
    27421 tagccggaac aatccgtgtt gattccattt ccgtttaatt acgtctccgg acgtttgtct
    27481 tcctgacaat cctctgtctt cgcctcctgg cgctcctgac cctcatcctg agtcattgtt
    27541 cctgttagcg tcctcgctct atgggtgcta ctttacgcct ttgctttaaa caaacaatca
    27601 accgccatca gtatttgcac attttcagag ttttatataa aagcaattca ctgatttata
    27661 tagcttattt gacatgcatc gcagaatttt ctctgctatt tcgcataaag actatcggca
    27721 atgtctcaca aagcgtatgg cttttactta caacgctcac gccgaaaaat ccgcctttta
    27781 ttatggttca ggtataatcg ccacaaagct ttgactgacg gagacgaccg ctttgactaa
    27841 tttgaattat caacagacgc attttgtgat gagtgcgcct gatattcgcc acctaccttc
    27901 cgataccgga attgaagtgg cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct
    27961 gaacacgctg actaaccaga aaagcctggc tcgtacctca aaaaccccag ggcgcaccca
    28021 gcttatcaac ctgtttgaag tggctgacgg caagcgtctg gttgacttgc ctgggtacgg
    28081 ttatgcggaa gtcccggaag agatgaagcg caaatggcag cgtgcgctcg gcgaatacct
    28141 cgaaaaacgt cagagcctgc aaggtctggt ggtgctaatg gatattcgcc atccgctgaa
    28201 agatttggat cagcagatga ttgagtgggc ggtagacagc aatatcgccg ttctggtgct
    28261 gctgaccaaa gcggacaaac tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg
    28321 tgaagctgta ctggcgttta acggtgatgt gcaggttgaa acgttttctt cgttgaagaa
    28381 acaaggcgtg gacaagctgc ggcagaaact ggatacctgg tttagcgaga tgcagcctgt
    28441 agaagaaacg caggacggcg aataattttc ttgccttaat gcttgtgccg gatgtggcgt
    28501 atccggcccg taaattcaat agattacaat tctaccgttg gcctgataaa catagcgcat
    28561 caggcattac ggatcttttc tttcgcccaa taaaaaacgc cccagtcatt actgactggg
    28621 gcggctaaaa tattcagcca aatccgatta cgtgaagtaa aaggtctgaa agatagaaca
    28681 tcttacctct gtaccctacg cgactaactt tactcttttt tgttcagttc agaaagcact
    28741 ttttgtaatt ttttttcagt tgttgcatag gaatttctaa tagccatcac aaaacgtgac
    28801 agcttatgtt gcttacttac gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat
    28861 cccagttttc gccactcccc acttccacca gcaacggcac atccagacgg gtacagtttt
    28921 ccatcagttg atgaatctgc ttcgcgacgg catcaacatc atctttatga acttcaaata
    28981 ccagttcatc gtgtacctgc atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg
    29041 catcaacggc aatcatcgcc cgtttgataa tgtcggcggc ggttccctgc attggcgcgt
    29101 taatggctgc acgttcagcc gctgcacgac gagcaccatt gctggatttg atatccggca
    29161 gatacagacg gcgtccgtcc agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg
    29221 tgcgttccat atactccagc acgccagggt agcgttcgaa gtaaaggtcc atgtacttct
    29281 gcgcttcttt acgtggaatg ttcaattgcc gcgccagacc gaaagcactc atgccataaa
    29341 tcagaccaaa gttgatcgct ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg
    29401 gcaaaccaaa cacttctgcc gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg
    29461 tcagcaagcc tttgtcacgc gagagatgcg ccataatgcg cagttcaatc tgcgagtagt
    29521 ccgctgagac aatcacataa tcctctggcg caataaacgc ctggcggata cgacgacctt
    29581 cttcgttacg caccggaatg ttttgcaggt taggatcggt tgacgataaa cgtcccgttg
    29641 cggttactgc ctggtgataa gaggtatgca cacgcccggt tttcgggttg atcatcagcg
    29701 gcagcttgtc ggtgtaagtc gacttcagct tcgccagacc acgatactcc agaatcactt
    29761 ttggcaacgg atagtccagc gccagttctt ccagtacctc ttccgacgtt gacggcgcgc
    29821 cacccggcgt tttcttcagc ggtttaatac cctgtttttc aaacagaatg gtttgcaact
    29881 gcttggtgga agaaaggtta aattcttcac ctgcaatttc atgcgctttc ttttccagct
    29941 cagccagacg caaggtgagc tcttcagaat gattgtgcag cactttcgga tcgatcttca
    30001 caccgttacg ttcaatgcgt gaaagcactg gcaccagcgg catttcgata ttctcgaaga
    30061 cgttcaacgg ccctttgtgt ttttgcagat ccggccacat tttcagatgc aactgcaagg
    30121 tgacatcagc atcttcggcg gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg
    30181 tcagttgatt tttgccttta cccgcaatct cttcaaaagt gatggttttg tgcttcaacc
    30241 aacgttccgc gaggctgtcc atatcgtgac gcccggcaac gctattgaga atgtaggact
    30301 ccagcatggt atcaaacgca atcccacgca gctcaatgcc gtaattcgcc agaataccgc
    30361 gatcgtattt caggttttgc ccgaccttca gcgccttttc atcttccagc agcggtttta
    30421 gcaactcgag tgcacgctcg cgagagattt gatcgggcgc gtcaagataa tcatgagcaa
    30481 ccggaatata tgccgctacg cctggctcga tagcaaaaga aagcccgacc aggttagcag
    30541 agatgttatc aaggctgtcg gtttcggtat caaatgcaaa taccggcgct ttttccagct
    30601 tcgcaatcca cgctttcagt gtttcttcat caaggatggt gacgtagttg tcataagaaa
    30661 tcaccgttgc cgtcacttct ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg
    30721 ctggttttgc ccctttggcc tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt
    30781 tgaactcata ctttttgaac agccccaaca actcttccgc tgccggttgc tgcacttcca
    30841 gttgttcaca ggtcagctcc agttcaacgt cggttttaat cgtcgccagc tggtatgaga
    30901 gataagcaac ttctttgttt tgctcgagct tcgctgccat tgttttcgcg ccacggaagc
    30961 tcaacccagc aattttttct ggctcggcat acagcgtatc cagcccgcca agaccttgca
    31021 gcaatgcctg cgcggttttt tcaccgacgc ccggtacgcc aggaatgtta tcggaggagt
    31081 cacccatcag cgccaggaaa tcgatgatca gttctggcgg cacgccgtac ttattcacca
    31141 cctcttccgg tccgaggatg gtattcgtca tggtattgat aagcgtaata tttggcgtca
    31201 ccagctgcgc catatcttta tcgccagtgc tgatcagcac cggacgcccg gctttttcgg
    31261 cttcgcgcgc cagagtaccg ataacgtcgt ccgcttctac gccagaaacc gccagcagcg
    31321 gcagtcccat cgctttaacc atcgcgtgca ggggttcgat ttgtgctcgc agatcgttcg
    31381 gcattggcgg gcgatgtgat ttgtaatgtt caaacagttc atcacgaaag gtttttccct
    31441 tggcgtcaaa gaccaccgct gcatgcgtcg gtttatattg catgatcaga ctgcgcagca
    31501 tgttgaggac accatacatc gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa
    31561 acgcgtgata tgcgcgataa agataagatg aaccatctac aaggataagt ggattttggg
    31621 ggatctgaac cataatgtcc gtgcctgttt atcagattat gggtaaagga tgccacagac
    31681 aggatgaaaa catgagtttt tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat
    31741 cgctcgcaaa tcgtcatgtg gataactttg tgcataaatt atttacatcg cttttaattt
    31801 a
//
LOCUS       DAPHJE010000047        31798 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPHJE010000047 DAPHJE010000000
VERSION     DAPHJE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.047"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(205..1578)
                     /gene="argH"
                     /locus_tag="RH222_002182"
     CDS             complement(205..1578)
                     /gene="argH"
                     /locus_tag="RH222_002182"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV3797198.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1639..2415)
                     /gene="argB"
                     /locus_tag="RH222_002183"
     CDS             complement(1639..2415)
                     /gene="argB"
                     /locus_tag="RH222_002183"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV3797199.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2423..3427)
                     /gene="argC"
                     /locus_tag="RH222_002184"
     CDS             complement(2423..3427)
                     /gene="argC"
                     /locus_tag="RH222_002184"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV3797200.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3581..4732
                     /gene="argE"
                     /locus_tag="RH222_002185"
     CDS             3581..4732
                     /gene="argE"
                     /locus_tag="RH222_002185"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV3797201.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5084..7735
                     /gene="ppc"
                     /locus_tag="RH222_002186"
     CDS             5084..7735
                     /gene="ppc"
                     /locus_tag="RH222_002186"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV3797202.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7918..9651
                     /locus_tag="RH222_002187"
     CDS             7918..9651
                     /locus_tag="RH222_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV3797203.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9800..10650
                     /locus_tag="RH222_002188"
                     /pseudo
     CDS             9800..10650
                     /locus_tag="RH222_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(10637..10978)
                     /locus_tag="RH222_002189"
     CDS             complement(10637..10978)
                     /locus_tag="RH222_002189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV3797204.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10980..11858)
                     /locus_tag="RH222_002190"
     CDS             complement(10980..11858)
                     /locus_tag="RH222_002190"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV3797205.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11824..14121)
                     /locus_tag="RH222_002191"
     CDS             complement(11824..14121)
                     /locus_tag="RH222_002191"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV3797206.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14172..14492)
                     /locus_tag="RH222_002192"
     CDS             complement(14172..14492)
                     /locus_tag="RH222_002192"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV3797207.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14507..15586)
                     /locus_tag="RH222_002193"
     CDS             complement(14507..15586)
                     /locus_tag="RH222_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV3797208.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLISRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15895..18396
                     /gene="ptsP"
                     /locus_tag="RH222_002194"
     CDS             15895..18396
                     /gene="ptsP"
                     /locus_tag="RH222_002194"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3797209.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18408..19070
                     /gene="fsa"
                     /locus_tag="RH222_002195"
     CDS             18408..19070
                     /gene="fsa"
                     /locus_tag="RH222_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV3797210.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19081..20184
                     /gene="gldA"
                     /locus_tag="RH222_002196"
     CDS             19081..20184
                     /gene="gldA"
                     /locus_tag="RH222_002196"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV3797211.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20459..21076
                     /locus_tag="RH222_002197"
     CDS             20459..21076
                     /locus_tag="RH222_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV3797212.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21103..22008)
                     /gene="yijE"
                     /locus_tag="RH222_002198"
     CDS             complement(21103..22008)
                     /gene="yijE"
                     /locus_tag="RH222_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV3797213.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRAMRPTPFKYTLAIALLQTCGMIGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALVYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGVVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22102..24282)
                     /gene="katG"
                     /locus_tag="RH222_002199"
     CDS             complement(22102..24282)
                     /gene="katG"
                     /locus_tag="RH222_002199"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV3797214.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24611..25501)
                     /gene="metF"
                     /locus_tag="RH222_002200"
     CDS             complement(24611..25501)
                     /gene="metF"
                     /locus_tag="RH222_002200"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV3797215.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25850..28282)
                     /gene="metL"
                     /locus_tag="RH222_002201"
     CDS             complement(25850..28282)
                     /gene="metL"
                     /locus_tag="RH222_002201"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV3797216.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28285..29445)
                     /gene="metB"
                     /locus_tag="RH222_002202"
     CDS             complement(28285..29445)
                     /gene="metB"
                     /locus_tag="RH222_002202"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV3797217.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29722..30039
                     /gene="metJ"
                     /locus_tag="RH222_002203"
     CDS             29722..30039
                     /gene="metJ"
                     /locus_tag="RH222_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV3797218.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30223..30831
                     /locus_tag="RH222_002204"
     CDS             30223..30831
                     /locus_tag="RH222_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV3797219.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30976..31651
                     /locus_tag="RH222_002205"
                     /pseudo
     CDS             30976..31651
                     /locus_tag="RH222_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7686 a         8844 c         8054 g         7214 t
ORIGIN      
        1 tttatgggga gtgtgaaggt aaagaagaca caatgataaa aaggaatgtg acaaataatg
       61 gttctggttg ggcgttaaat ttaagctcac cagaatgctg acaaatttct gcctgatgtg
      121 gcgtaaacgc cttatccgcc tacgaagtgt cgcactttgt aggcgtggta agctccccat
      181 tttcagggga gcaatataaa caaattactc taaccgcgcc tgcgcaaaag caattgcctg
      241 cgccacctgc tgcggtgaga cgccgccttt tgccgcacgc ttgtcgaggc acgattgcag
      301 cgacagaatc ggatagacat cttcgtcaat caccgggctg aatttctgca actcgtcgag
      361 cggcagatct tccagcggtt tgccctgacg aatggcttcc accaccgctt caccaacaat
      421 atggtgcgcc tcgcggaacg gtacgccttt cgccaccaga taatccgcca gttcggtggc
      481 gttggcgtaa ccctgctgag ccgcttcctg gcaacgtgga cgtttcacct gaatgccgtc
      541 cagcaccagc gccgccatat gcaggcagtc cagccaggta tcgagcgcgt cgaacagacc
      601 ttctttgtct tcctgcatat ctttgttgta agccagcggc aaacctttca gcgtcatcat
      661 catgccggtt aacgccccct gcacccgacc gcatttaccg cgaatcagct ccagcgcatc
      721 cgggtttttc ttctgcggca ttaatgatga accggaagtc acgcggtcag aaagctccac
      781 aaaccccgct tcgccggtgt taaagaaaat cagatcttca gcaaaacgcg acagatgcac
      841 catgccgata gcggcagcag aaagcagttc caacacatgg tcacggtcag aaacgctgtc
      901 gagactgtta cgggtcgccg aagcaaagcc cagccagcct gctaactgtt cacggtcgat
      961 ttcataggcc gttcccgcca gcgcgccaca gcctagcggg ctgacatcca gacgcttaag
     1021 cgcatcctgc aaacggcttt catcacgcgc cagcatctca acataggcca ggcaccagtg
     1081 cgcgaacgtc accggctggg cgcgttgcag gtgagtgtaa cctggcatta ccgcgtcctg
     1141 attgttttgt gcggtttcca ccagcgccga ttgcagctgc cggttagccg tcagtaactc
     1201 gctaacggta tctttgcacc acagtttcag gtcagtcgct acctgatcat tacggctacg
     1261 cccggtatgc agctttttgc ctaactggcc cactttgtcg atcagtttgc cttccaccca
     1321 gctatggata tcttcggcgt cgctttcaag gatttgttgt ggcctggcgc gaacatcttc
     1381 cagcaacacg ttcagcgcct cttccagttg cgcctgctct tctgcggtta acacgcctac
     1441 cgtgaccagg gctttggacc aggccacaga gccaacaata tcctgctccg ccagacggta
     1501 atcaaagcgc agtgagtcgt tgaattgttt gaaccgttga tctgctgcct gggtaaaacg
     1561 cccgccccaa agtgccataa ctctgtttcc ttattttgaa attcaatgcc ggaaagctgc
     1621 gcctccggcc aacaaaactt aagctaaaat ccgcgtaccc atcggcatac cgttaaacag
     1681 tgccggaagc tgctccgcat gacgccagga ggcgatatct accggacggc ccagcgtgcg
     1741 ggccgcatcc agcgccgcgt tcactttcac tatcatgccg tcagtaataa tgccctgctc
     1801 aatcagttgt tctgctttcg cggcggtcat ttcggcaatg cgttgccctt tgccgtcgag
     1861 aatgccgctg acgtcggaga gcaaaatcag atccgcgccc agcgttgccg ccagcgccgt
     1921 tgccgcctgg tcggcattga cgttcatcag ttgcccttcg tctgttacgc caatggagct
     1981 gaccaccggc agataaccgt tctccagcaa ggagttgata agcttaggcg aacctggctg
     2041 cgccagtcca acatgaccta actcttcatc aagctgggtc actttgacgc tgtcgccgtc
     2101 accgagaaac aaacctacgg ccgcaatctg atgtttcttc gcccatgcca acagggtttt
     2161 atttgccgtt cccgccagtg ctccggtgat aatgtctatc tgatcagcag gcgtcacccg
     2221 caggccgttt ttctttttca ccggcagatt cagccctttc atcagctcat ccaccacgca
     2281 accgccgccg tgcacaatca ccagcggacg ctgatgtgac tcacgataat tcaccagtgc
     2341 gctaaacaga cgttccagcg cctcttcact atccagcagt acgccgccca gtttgataat
     2401 taatggattc atcattgcac ccttaaataa gagactgcgt ttccgcatag ccgaaacgaa
     2461 tattggcgca ctgtaccgct tgtgccgccg cgcctttcag taagttgtct tcggtcgcca
     2521 caataatcag atgctcgccc tgaacggcaa acccgatatc gcaaaatggc agcccaacga
     2581 catttttcag cgccggaacg cctttgtcat acagccgcac cagcggttta tgggcatacg
     2641 cctgctgtaa cacttgcgcg acttgcgcct gggtcacacc cgatttcagg cggcaggtaa
     2701 tggtttcgag aatgccgcgc gggaaattgc ccagatgtgg ggtgaagata acgtcagcac
     2761 cgaggtgtgt ggcgatctct ggttgatggc gatgagtaaa gacgccatac ggttgcaggc
     2821 taacttcaca aaagctgttt gaaatggccg ctttacgccc tgcaccgctc acgccgctgg
     2881 tggcgttgat caccggccac tgattgaggt caagaagatc ggcatcaatc aacggtttca
     2941 gcgccagctg tgccgccgtc ggataacagc ccggcaccgc aatcaaattc gcttctttta
     3001 atttattacc gcaccactcc gccagaccgt aggctgcctg ttccaacagt tccgggtatt
     3061 gatgggtaaa gccgtaatat ttttcataga aggtggcgtc gttaacacga aacgcgccgg
     3121 aaaggtcgaa caccacgcag cccgcttcaa gaaactgcgg cgctaaatcg tggctaactt
     3181 catgggcggt ggcgagaaac actacgtcca cccctgggct aaactcgctg atatccgaca
     3241 tcggctgcaa cggcagatca acgatgcctt ttagctgcgg atgcaaatcg gagattaact
     3301 ttcccgcatc attgctttgc gctgaaacag tcaaagcggt tatgttcata tgcggatggc
     3361 gatttacata ggtcactagc tctgcgccag cgtagccgct ggcacccaca atcagcgtat
     3421 tcaacatcgg ggctattcac cttcttatgt ctggttgcca ggttaaacgt aaaacattca
     3481 ccttacggct ggtgggtttt attacgctca acgttagtgt atttttattc ataaatactg
     3541 catgaatatt gatactatca tgaccagagg tgtgtcaaca atgaaaaaca aattaccgcc
     3601 atttatcgag atttaccgcg ctctgattgc cacaccttca ataagcgcca cggaagaggc
     3661 actcgatcaa agcaatgcag atttaatcac tctgctggcg gactggttta aagatttggg
     3721 cttcaatgtg gaagtgcagc ctgttccagg aactcgcaac aaatttaata tgctggccag
     3781 ttgcggacag ggggctggcg gcttgttgct ggcggggcat accgatacgg tgccatttga
     3841 tgacggtcgc tggacgcgcg atccgtttac actgacggag catgacggca agctttacgg
     3901 cttaggcacc gccgacatga aaggcttttt tgcgtttatc cttgatgcgc tacgcgatgt
     3961 cgacgtcacg aaactgaaaa aaccgctcta cattctggcg actgctgatg aagaaaccag
     4021 tatggccgga gcgcgttatt ttgccgaaac taccgccctg cgcccggatt gcgccatcat
     4081 tggcgaaccg acgtcactac aaccggtacg cgcacataaa ggtcatatct ctaacgccat
     4141 ccgtattcag ggccagtcgg ggcactccag cgatccagca cgcggagtta acgctatcga
     4201 actaatgcac gacgccatcg ggcatatttt gcaattgcgc gataacctga aagaacgtta
     4261 tcactacgaa gcgtttaccg tgccataccc tacgctcaac ctcgggcata ttcacggtgg
     4321 cgacgcttct aaccgtattt gcgcttgctg tgagttgcat atggatattc gtccgctgcc
     4381 tggcatgaca ctcaatgaac ttaatggttt gctcaacgat gcattggctc cggtgagcga
     4441 acgctggccg ggtcgtctga cggtcgacga gctgcatccg ccgatccctg gctatgaatg
     4501 cccaccgaat catcaactgg ttgaagtagt tgagaaattg ctcggagcaa agaccgaagt
     4561 ggtgaactac tgtaccgaag cgccgtttat tcaaacgtta tgcccgacgc tggtgttggg
     4621 gcctggctca attaatcagg ctcatcaacc tgatgaatat ctggaaacac ggtttatcaa
     4681 gcccacccgc gaactgataa cccaggtaat tcaccatttt tgctggcatt aaaacgtagg
     4741 ccggataagg cgctcgcgcc gcatccggca ctgttgtcaa actccagtgc cgcaataatg
     4801 tcggatgcga tacttgcgca tcttatccga cctacacctt tggtgttact tggggcgatt
     4861 ttttaacatt tccataagtt acgcttattt aaagcgtcgt gaatttaatg acgtaaattc
     4921 ctgctattta ttcgtttgct gaagcgattt cgcagcattt gacgtcaccg cttttacgtg
     4981 gctttataaa agacgacgaa aagcaaagcc cgagcatatt cgcgccaatg cgacgtgaag
     5041 gatacagggc tatcaaacga taagatgggg tgtctggggt aatatgaacg aacaatattc
     5101 cgcattgcgt agtaatgtca gtatgctcgg caaagtgctg ggagaaacca tcaaggatgc
     5161 gttgggagaa cacattcttg aacgcgtaga aactatccgt aagttgtcca aatcttcacg
     5221 cgctggcaat gatgctaacc gccaggagtt actcaccacc ttacaaaatt tgtcgaacga
     5281 cgagctgctg cccgttgcgc gtgcgtttag tcagttcctg aacctggcca acaccgccga
     5341 gcaataccac agcatttcgc cgaaaggcga agctgccagc aacccggaag tgatcgcccg
     5401 caccctgcgt aaactgaaaa accagccgga actgagcgaa gacaccatca aaaaagcagt
     5461 ggaatcgctg tcgctggaac tggtcctcac ggctcaccct accgaaatta cccgtcgtac
     5521 actgatccac aaaatggtgg aagtgaacgc ctgcttaaaa cagctcgata acaaagatat
     5581 cgctgactac gaacacaacc agctgatgcg tcgcctgcgc cagttaatcg cccagtcatg
     5641 gcataccgat gaaatccgta agctgcgtcc aagcccggta gatgaagcca aatggggctt
     5701 tgccgtagtg gaaaacagcc tgtggcaagg cgtaccaaat tacctgcgcg aactgaacga
     5761 acaactggaa gagaacctcg gctacaaact gcccgtcgaa tttgttccgg tccgttttac
     5821 ttcgtggatg ggcggcgacc gcgacggcaa cccgaacgtc actgccgata tcacccgcca
     5881 cgtcctgcta ctcagccgct ggaaagccac cgatttgttc ctgaaagata ttcaggtgct
     5941 ggtttctgaa ctgtcgatgg ttgaagcgac ccctgaactg ctggcgctgg ttggcgaaga
     6001 aggtgccgca gaaccgtatc gctatctgat gaaaaacctg cgttctcgcc tgatggcgac
     6061 acaggcatgg ctggaagcgc gcctgaaagg cgaagaactg ccaaaaccag aagggctgct
     6121 gacacaaaac gaagaactgt gggaaccgct ctacgcttgc taccagtcac ttcaggcgtg
     6181 tggcatgggt attatcgcca acggcgatct gctcgacacc ctgcgccgcg tgaaatgttt
     6241 cggcgtaccg ctggtccgta ttgatatccg tcaggagagc acccgccata ccgaagcgct
     6301 gggcgagctg acccgctacc tcggtatcgg cgactacgaa agctggtcag aggccgacaa
     6361 acaggcgttc ctgatccgcg aactgaactc caaacgtccg cttctgccgc gcaactggca
     6421 accaagcgcc gaaacgcgcg aagtgctcga tacctgccag gtgattgccg aagcgccgca
     6481 aggctccatt gccgcctacg tgatctcgat ggcgaaaacg ccgtccgacg tactggctgt
     6541 ccacctgctg ctgaaagaag cgggtatcgg gtttgcgatg ccggttgctc cgctgtttga
     6601 aaccctcgat gatctgaaca acgccaacga tgtcatgacc cagctgctca atattgactg
     6661 gtatcgtggc ctgattcagg gcaaacagat ggtgatgatt ggctattccg actcagcaaa
     6721 agatgcggga gtgatggcag cttcctgggc gcaatatcag gcacaggatg cattaatcaa
     6781 aacctgcgaa aaagcgggta ttgagctgac gttgttccac ggtcgcggcg gttccattgg
     6841 tcgcggcggc gcacctgctc atgcggcgct gctgtcacaa ccgccaggaa gcctgaaagg
     6901 tggcctgcgc gtgactgaac agggcgagat gatccgcttt aaatatggtc tgccagaaat
     6961 caccgtcagc agcctgtcgc tgtacaccgg ggcgattctg gaagccaacc tgctgccacc
     7021 acctgagccg aaagagagct ggcgtcgcat tatggatgaa ctgtcagtca tctcctgcga
     7081 tctctaccgt ggctacgtac gtgaaaacaa agattttgtg ccttacttcc gctccgctac
     7141 gccggaacaa gaattgggca aactgccgtt gggttcacgt ccggcgaaac gtcgcccaac
     7201 cggcggcgtc gagtcgctgc gcgccatacc gtggattttt gcctggacgc aaaaccgcct
     7261 gatgctcccc gcctggctgg gtgcaggtac ggcgctgcaa aaagtggtcg aagatggcaa
     7321 acagagcgag ctggaagcca tgtgccgcga ttggccattc ttctcgacgc gtctcggcat
     7381 gctggagatg gtcttcgcca aagcagacct gtggctggcg gaatactatg accaacgcct
     7441 ggtagacaaa gcactgtggc cgttaggtaa agagttacgc aacctgcaag aagaagacat
     7501 caaagtggtg ctggcgattg ccaacgattc ccatctaatg gccgatctgc cgtggattgc
     7561 agagtctatt cagctacgga atatttacac cgacccgctg aacgtattgc aggccgagtt
     7621 actgcaccgc tcccgccagg cagaaaaaga aggccaggaa ccggatcctc gcgtcgaaca
     7681 agcgttaatg gtcactattg ccgggattgc ggcaggtatg cgtaataccg gctaatcttc
     7741 ctcttctgca aaccctcgtg cttttgcgcg agggttttct gaaatacttc tgttctaaca
     7801 ccctcgtttt caatatattt ctgtctgcat tttattcaaa ttctgaatat accttcagat
     7861 atccttaagg aattgtcgtt acattcggcg atattttttt caagacaggt tcttactatg
     7921 cattccacag aagtccaggc taaacccctt tttagctgga aagccctggg ttgggcactg
     7981 ctctactttt ggtttttctc tactctgcta caggccatta tttacatcag tggttatagt
     8041 ggcaccaacg gcattcgcga ctcgctgtta ttcagctcgc tgtggttgat cccggtattc
     8101 ctctttccga agcggattaa aattattgcc gcagtaatcg gcgtggtgct atgggcggcc
     8161 tctctggcgg cgctatgcta ctacgtcatc tacgggcagg agttctcgca gagcgttctg
     8221 tttgtgatgt tcgaaaccaa caccaacgaa gccagcgagt atttaagcca gtatttcagc
     8281 ctgaaaattg tgcttatcgc gctggcctat acggcggtgg cagttctgct gtggacacgc
     8341 ctgcgcccgg tctatattcc aaagccgtgg cgttatgttg tctcttttgc cctgctttat
     8401 ggcttgattc tgcatccgat cgccatgaat acgtttatca aaaacaagcc gtttgagaaa
     8461 acgttggata acctggcctc gcgtatggag cctgccgcac cgtggcaatt cctgaccggc
     8521 tattatcagt atcgtcagca actaaactcg ctaacaaagt tactgaatga aaataatgcc
     8581 ttgccgccac tggctaattt caaagatgaa tcgggtaacg aaccgcgcac tttagtgctg
     8641 gtgattggcg agtcgaccca gcgcggacgc atgagtctgt acggttatcc gcgtgaaacc
     8701 acgccggagc tggatgcgct gcataaaacc gatccgaatc tgaccgtgtt taataacgta
     8761 gttacgtctc gtccgtacac cattgaaatc ctgcaacagg cgctgacctt tgccaatgaa
     8821 aagaacccgg atctgtatct gacgcagccg tcgctgatga acatgatgaa acaggcaggt
     8881 tataaaacct tctggatcac caaccagcag acgatgaccg cccgcaatac catgctgacg
     8941 gtattttcga agcagaccga caaacagttc tatatgaacc agcaacgtac gcagagtgcg
     9001 cgtgaatacg acaccaacgt gctgaaacca ttccaggaag tgctgaagga cccggcgccg
     9061 aagaaactga tcatcgttca tctgctgggt acgcatatca aatacaaata ccgctacccg
     9121 gaaaatcagg gcaagtttga tggcaatacc gatcatgttc cgcctggatt aaacgcggaa
     9181 gagctggagt catataacga ttatgacaac gctaacctgt ataacgatca tgtggttgcc
     9241 agcctgatta aagactttaa agcagcaaac ccgaacggtt tcctggttta tttctctgac
     9301 cacggtgaag aggtttacga cacgccgccg cacaaaactc aggggcgtaa cgaagacaac
     9361 ccgacgcgcc acatgtacac cattccgttc ctgctgtgga cgtcggaaaa atggcaagcg
     9421 actcatccgc gtgatttctc gcaggatgtt gatcgtaaat acagcctggc ggaactgatc
     9481 cacacctggt cagatttagc aggcttatct tacgacggtt acgatccaac ccgttcagtg
     9541 gtgaatccgc agttcaaaga aactacccgc tggattggta acccgtacaa gaaaaacgcg
     9601 ctgatcgatt acgacacgct gccgtatggc gatcaggtgg gtaatcagta aatgaccttt
     9661 gtatcgcgtc tggcgctatt tgccggatgc gatactgccg caatgtttac atcgaactcc
     9721 cctcaggcca cacttttcag gtggcctttt ttaataatgc cagttactgt tttcccgtct
     9781 acatacctaa gggaataaca tgtatcacga cgtcagctat ctgctctccc gcctgatcaa
     9841 cggcccgctg tccctgcgcc agatttactt tgccagcagt aacggcccgg tgcccgatct
     9901 cgcgtatcag gtcgattttc cacggctgga aattgtgctg gaaggtgagt ttgttgatac
     9961 cggcgctggg caacgttagt tcccggcgat gtgctgtacg ttcctgctgg tggctggaat
    10021 tttccacaat ggcaagcccc cgctactacc tttagcgtgc tgtttggcaa acagcaactc
    10081 ggcttcagcg tcgtgcaatg ggatggcaaa caatatcaaa atctggcgaa gcaacacgtc
    10141 gcccggcgcg gcccacgcat aggttctttt ctgctacaaa cgctcaatga aatgcaaatg
    10201 cagccgcagg agcagcaaac ggcaaggctt atcgtcgcca gcctgcttag ccactgccgc
    10261 gatttgctcg gcagccaaat ccagaccgcc tcacgcagcc aggcactatt tgaagctatt
    10321 cgcgattata tcgacgaacg ctatgcctcc gcgcttaccc gcgaatctgt cgcacaggca
    10381 ttctatattt caccaaacta tctctcgcac ctgtttcaaa aaacgggggc cattggtttt
    10441 aacgaatacc tgaatcacac gcgactggag cacgctaaga cgttattgaa aggctatgac
    10501 ctgaaggtaa aagaagtggc gcacgcctgc ggctttgtcg acagcaacta cttctgccgt
    10561 ctgtttcgta aaaacaccga acgctcgccg tcggaatatc gtcggcagta ccacagccaa
    10621 ctgacagaaa aaccgactac tccagaataa gatgggtttg ctgcggcgca gaaagcactt
    10681 tgcgcaccgc gctcattacc cgctgcggct cacgcaggaa tgcgtagatg ctgcattgca
    10741 catagcggca atgttcaaat cgctcggcac cggcaagctc gatatccgta atcaacagag
    10801 caacatccgc ccgacggata tcctcgtccg ctaaacgatt ctccgttccc agcgcgccct
    10861 gagtttcaat gctgacgccc cacttctcta actggcacaa cttttccagc cgttccgccg
    10921 ccatataagt atgcgccacg ccgcttacgc aggcggttac tgccaccagg tatgccattt
    10981 taacctccca cggtaacctg aaatccggcc cgttctgcca tttcgcgcat cgttgtcaca
    11041 tcggctgacg acggcgcagg cacctctttc atcgaccatg ttttccccag caggcggtat
    11101 ttcggttcgc cgtactgatg aaacggtaac agatggatct gcctgatttt cagcgggatc
    11161 agtacatcca gcgcctgctg catattctcc cggctgagcg tgaaaccagg gatcaacggt
    11221 aaacgcggga tcacgttgac accctcactc accagcaaac gcagattctc cagcacgcgt
    11281 ggcaggttca tcttcaccac atcccgcgcc tgagtcgcgt ccataatttt taaatcgaac
    11341 agcacttcat cgcacaattt cgccagtggt aacagcttgc tggctggcgc gtctccggca
    11401 gtttcaatgg cgcatgacac gccccacaac cgcagccgct gtaaaaaacg ggtagcaaac
    11461 tccgcctgca ttaacacttc gccgccagaa agcgtgacgc cgccgccgga cgtgcgaaag
    11521 aaaatgtcat ctttcatcac ttcccgttcc agcgcgtcaa ggctgatatc gcgaccaatc
    11581 cgttcaaacg ccccggaggg gcattcatcc gcatcacgca aacatttcgc acagtgcaga
    11641 catttcgcct ctctgcgtac cgtctggatt ttgccggaga tcgactccgg attagcacac
    11701 catgggcaaa gatgcggaca gcctttaaaa aagaccaccg tacgaatgcc ctcgccgtca
    11761 ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat cattgcggcg cgtttccaca
    11821 acgttacagc tgatgcgctg tccggcggat gatgtcatcc tggatctcct tcgacagttc
    11881 gacaaagaag gcgctgtatc cggcaacgcg caccaccagc ccggcataat cttgtgggcg
    11941 ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta aactgaatat gctgcaactt
    12001 aagctgggta aacgcccgta agaagtcggc cagtttgcgt aatcccgctt caccttccag
    12061 ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta gacagcagcg tattatcgag
    12121 cttactgact gacttcagta ccgctgttgg cccttgtgcg tcctggccca gcataggtga
    12181 caagccgccg tctgccagtt gttccccggc aaaacgaccg tctggcgtcg cgccaaccac
    12241 cgatcccaac gggacgtgag cagaaacggt atacgatccc ggcgtgaagt aaccaccgcg
    12301 cgggttctgg tatttttcca cttctttgca gtagtggcgt aacagttcgg cgctgatgtt
    12361 atccacctcg tcgatatcgt taccgtattt ctcgaagcgg ttaattaagc gagcacggac
    12421 tttttcgcct tctggcgttg caaagttggc ttttaatacc gacagcaatt cgtcaaaact
    12481 taaacgctgt tgctcaaaaa ccatcccttt caacgcatgg agagaatcgc tcaggttggc
    12541 gataccgatc ccctgtacgc cggagaagtt ataacgcgcg ccgccatcgg taatatcgcg
    12601 acctttttcc agacaatcgc tgataaaaga cgagagcagc ggtacaggtg cccagtcgcg
    12661 atggccgata tcacaaatat tgctgccctc aaccatcagg gtgatgtagt ggctgatctt
    12721 ggcgcggatc tgctccagta aaccttcata agtcagcgca gcattgcctt cattttcatg
    12781 caggcagatt tccatcactt tcagcagatt aaacatcgcg atgtcatgca agccgtaggt
    12841 tctgccggga atagataatt ccacacagcc cactacggaa tagtcgcgcg catcttccag
    12901 cgaaactcca cggttgagga acgctggtac caccacttca tcgttaaaga tttgcggaat
    12961 accggtgccg aggcgaatgg tttcggcggt tttcatcagg aacggcgtgt cgatcaaggc
    13021 gttagtgcgc acgccgaggt tcggttgcgg taattgcacg ctttgataag cgtcaaggca
    13081 aaggaacgaa agcacgttca ccgcgctacg tccgttctcg gttaacccgc cgagcagtgc
    13141 ggtatagccg gtcgggaaac ctgcgaaata acgcgcgctg ctggtggagc gcaacagcac
    13201 gatgtcgttg catttcaccc ataaagattc gagtagttct ttcaggaacg ccggatcttc
    13261 gccctgggtt aaagatgcct gatagaacgg caacatatac tggtcgaagc gccccaacga
    13321 tagcgaactg gcgttggatt cgtattgcag aatgatgttc atgtaccaga ataactggca
    13381 cgcctgccag aacgtctgcg gcttatgttc ggcgttatgg cgggagatct ccgcaatagt
    13441 cagcagttct tcgcgacgct gggcatctgt gcagtttgcc gccattgttt ccgccagttc
    13501 agcgtaacgc agaatgtgtt tctgcgaggc ttccagcagt aacagcgcgg cctgataaaa
    13561 gtgattctcc ggctgttgct gacaatgttg ctgcatctgc gccaccagct cccccagccc
    13621 gtgattcagc aggcgtgggt aatcaataat aatgtgcccc tggcctttat ctgtctggtt
    13681 gatgctgaaa atctgcgtgc tggtcgcggc ttttacttca tctgtcatct gcccgttgat
    13741 gaaatctttc atcgaacgtt tttcccagta cgggaacaac tcttcacggt agatacgttt
    13801 atcttcttca ctgatagcaa agcggtcctg cggacgcgtc gggaattgat ccagctcttt
    13861 cagcagccag taagggtcca tttccggcga cataatccca gcgcgcggtt ttacggtgcg
    13921 gttaccggca atcagttctt catcacgaat cgaaatttca acatgttcaa ggatatacgc
    13981 tgttgctttc gcccggcgca atatcaccgg ttcgccttcg gtttgccgat ggctggcggt
    14041 ataaagcagc gcccgctcca gcgagatttc acgggtattg gcaaacagtg cagttttgag
    14101 gcgagagata cgattcgtca tgaggaactc ctgtgggaaa gcgaacccgc ccttgcgagc
    14161 gggagaagaa cttaagcggt ttgcgccagg tgagcttcaa ttttgctcat aatggcgtca
    14221 gcacgtttta ctgcatcgct gatgttgacg cgcacgatgg tcttgccagc aaaacgctct
    14281 tcaaacttga tgccgatatc tttggtcaga atcaccatat cagcgctcgc cacatcttcc
    14341 gcagtcagtt cattttccag accaattgac ccctgggttt ctactttcac ttcccagcct
    14401 ttcgctttcg cggcactttc cagcgcctct gctgccatat aggtatgggc aacacctgaa
    14461 ggacatgcgg ttactgcaat aattttcgtc atggctcgtt cctcaattaa ttaatttcaa
    14521 aatccaaatc caggtcgtct tctttttcat cagtcgaaga cccattttta cgcgccagac
    14581 ttttcagcac gttcacacaa acagccgtca ccactgctcc gacagccact gctgcgatat
    14641 aacccagctt gccttcaacc accggcagca caatcagacc accccagcct gcatagcact
    14701 gcgcaccgac cagcgccgca gttactgcac cacaaactga accgaccatg atggaaggaa
    14761 tgacacgcag cggatcggca gcggcgaaag gaatcgcccc ttcagtaacg ccaacacagc
    14821 ccatcaccag tgccgctttg ccggtttcgc gctcttctgc ggagaaattt ttacgactaa
    14881 tcagcgtcgc cagccccatc cccagcggcg ggatacaaat acccacggca gcgatggcca
    14941 ccacggtata aacaccctga gcaacgcaaa tcagcatgaa ggcataggcc actttgttaa
    15001 ccggaccgcc catatcgaac gccagcatca gacccatgat caccgccagc ataacaatgc
    15061 tgccctgctg catcccctga agccactgag tcaggctgtt ggtcaacgcc cctacaggct
    15121 cgcccaagcc ccacatcatg atgcctgcgg taatcagtgt gccgacgata ggaataatga
    15181 agataggcat caccgagcgc agaactttat gcaccggaat tttcttcagg taatgcacca
    15241 cgatgccgcc gataatcccg gcgatcagtg caccaaagaa gcccgcacca aagctgttac
    15301 caacccaggc accgatagcg cacggagcca gcgcagaacg ctctgcaatg gaataaccga
    15361 tgtacgctgc gaggaaaggc accatcagcg tcaagcccgc aacgccgata tcaaacagtt
    15421 ttttcagatt tggatcggct acggcatccg gcactgcgcc tttgccatac aacatgacgg
    15481 aaaccgccag caaaataccg cccgatacca cgaagggaat catgtgtgaa acgcccgtca
    15541 ttaaatgctg acgggtattt ttcaggatct gcaccaactc attcataagc tgctcccggg
    15601 cttgcgaaag cccatgtcca tatttttgat ggaggaaaca ataggcaatc gcaggcggag
    15661 caaacagtgg ataaaaaggc catttactgg acttttgtaa tatcagtaca aaaatgcgat
    15721 ccgcctcata acttgcgata aaccggaagc atgccgcaaa acagcgtctt gtgagacgcg
    15781 tcgcaaattg acggttacat tacatttgtc cagcttttgg cagatttgtc acatggcagg
    15841 ccgttgccgt gcgctcttat cctgtaaaca gaaaaccgta ccgggagaga ggcaatggcc
    15901 ctgattgtgg aatttatttg tgagctacct aacggcgtac atgcacgtcc ggcaagccac
    15961 gttgaaacgc tgtgtaatac tttttcatca caaattgagt ggcataacct gcgcactgac
    16021 cgcaagggca acgccaaaag cgcccttgcg ctgattggca ccgatacgct ggcgggcgat
    16081 aactgccagt tactgatttc cggggccgac gaacaggaag cgcaccagcg tttaagccaa
    16141 tggctgcgcg atgaattccc ccactgcgat gcgccgctgg cggaagttaa atctgacgaa
    16201 ctggaaccac tgccggtttc actgaccaat ctgaatccgc aaattatccg cgcccgcacc
    16261 gtgtgcagcg gtagtgcagg cggcattctg acgccgatct cttctttaga tctcaatgcg
    16321 ctgggtaatc ttcccgcagc caaaggcgtt gacgccgagc aatccgcact ggaaaacggc
    16381 ctgacgctgg tactgaaaaa cattgagttt cgtctgctgg atagcgacgg tgccaccagc
    16441 gcgattctgg aagcgcaccg atccctggct ggcgatactt ccctgcgcga acatttactg
    16501 gcaggcgtca gcgccggatt aagctgcgcc gaagcgattg tcaccagtgc gaatcacttt
    16561 tgcgaagagt tcgcccgttc cagcagcagc tacctgcaag aacgtgccct ggacgtacgc
    16621 gacgtctgct tccagttact ccagcaaatc tacggtgagc aacgcttccc ggcaccgggc
    16681 aaactgacgc agcccgccat ttgtatggct gatgaactga ctcccagcca gttcctcgaa
    16741 ctggataaaa atcacctcaa aggtctgtta ctgaaaagtg gcggcaccac ctcacatacc
    16801 gtaattcttg cccgttcgtt caacattccg acactggttg gtgtagatat tgatgccctt
    16861 actccgtggc agcatcaaac gatttatatc gacggcaacg ctggggcgat tgtggttgag
    16921 ccaggagaag ccgtagctcg ttattatcag caagaagccc gcgtacagga cgccctgcgt
    16981 gagcaacagc gtgtctggct gacccaacaa gcccgtaccg ctgacggtat ccgcattgaa
    17041 attgccgcta acatcgctca ctccgtggaa gcgcaggccg cattcggcaa tggtgcggaa
    17101 ggcgttggtt tgttccgcac tgaaatgctc tatatggatc gcaccagtgc accgggcgaa
    17161 agcgagctgt acaacatttt ttgtcaggcg ctggagtctg ccaacggacg cagcattatt
    17221 gtgcgcacta tggacattgg cggcgacaaa cccgttgatt atctgaacat tccggcagaa
    17281 gctaacccgt tcctcggtta tcgcgctgtg cgtatttatg aagaatacgc ctcgctgttc
    17341 accacacaac tacggtcgat cctgcgcgcc tccgctcacg gcagcctgaa aatcatgatc
    17401 ccgatgatct cctcaatgga agagatctta tgggtgaaag aaaaactggc ggaagccaaa
    17461 cagcaactgc gtaacgaaca cattccgttt gatgagaaga tccagcttgg cattatgctg
    17521 gaagtaccgt cggtgatgtt catcatcgat caatgctgcg aagagattga tttctttagt
    17581 attggtagta atgacctgac gcaatatctg ctggcagtgg atcgcgataa cgctaaggtt
    17641 actcgtcact acaacagcct gaatccggca ttcttgcggg cgctcgatta cgccgtgcag
    17701 gcggtgcatc gccagggcaa atggattggt ctgtgcggtg agctgggagc gaaaggttcc
    17761 gtgctgccgt tgctggtcgg cttagggctg gatgaactca gcatgagcgc accatcaatt
    17821 ccggcggcga aagcgcggat ggcgcaactt gatagccgtg agtgccgcaa gttgctcaac
    17881 caggcaatgg cctgccgtac ttcgctggaa gtggaacacc tgctggcgca attccgcatg
    17941 acccaacagg acgcaccgct ggtcaccgcc gagtgcatca cgctggaaag tgactggcgc
    18001 agcaaagaag aagtgctcaa aggcatgacc gataacctgc tgctggcggg ccgctgccgc
    18061 tatccgcgta aactggaagc cgacttatgg gcgcgcgagg ccgttttctc taccggtctg
    18121 ggctttagtt ttgccattcc acacagcaaa tcagaacaca ttgagcaatc caccatcagc
    18181 gtggcgcgtc tgcaagcgcc ggtgcgctgg ggcgatgatg aagcgcaatt catcattatg
    18241 ttaaccctga acaaacacgc tgcgggcgat cagcacatgc gcattttctc gcgcctcgct
    18301 cgccgcatca tgcacgaaga attccgtaac gcgctggtta acgccgcctc tgccgacgct
    18361 atcgccagcc tgctgcaaca tgaactggaa ctgtaaaagg aaacatcatg gaactgtatc
    18421 tggacaccgc taacgtcgca gaagtcgaac gtctggcacg catattcccc attgccgggg
    18481 tgacaactaa cccgagcatt atcgctgcca gcaaggagtc catatgggaa gttctgccgc
    18541 gtctgcaaaa agcgattggt gatgagggca ttctgtttgc tcagactatg agtcgtgacg
    18601 cgcagggtat ggtggaagaa gcgaagcgcc tgcgcgacgc cattccgggc attgtggtga
    18661 aaatcccggt gacttccgaa ggtctggccg ccattaagat gctgaaaaaa gagggtatta
    18721 ctacgcttgg caccgcagtg tacagtgctg cacaagggtt actggccgcg ctggcagggg
    18781 caaaatacgt tgctccgtat gttaaccgcg tagatgccca gggcggggac ggcattcgta
    18841 cggttcagga gctgcaagcg ctgttagaaa tgcacgcacc agaaagcatg gtgctggcgg
    18901 ccagctttaa aacgccgcgt caggcgctgg actgtttact ggcaggatgt gaatccatca
    18961 ccctgccctt agatgtagcg caacaaatgc tcaacacccc tgcggtagag tcagctatag
    19021 agaagttcga acacgactgg aatgccgcat ttggcactac tcatctctaa aggagcaatt
    19081 atggaccgca ttattcaatc accgggtaaa tacatccagg gcgctgatgt gattaatcgt
    19141 ctgggcgaat acctgaagcc gctggcagaa cgctggttag tggtgggtga caaatttgtt
    19201 ttaggttttg ctcaatccac tgtcgagaaa agctttaaag atgctggact ggtagtagaa
    19261 attgcgccgt ttggcggtga atgttcgcaa aatgagatcg accgtctgcg tggcatcgcg
    19321 gagactgcgc agtgtggcgc aattctcggt atcggtggcg gaaaaaccct cgatactgcc
    19381 aaagcactgg cacatttcat gggtgttccg gtagcgatcg caccgactat cgcctctacc
    19441 gatgcaccgt gcagcgcatt gtctgttatc tacaccgatg agggtgagtt tgaccgctat
    19501 ctgctgttgc caaataaccc gaatatggtc attgtcgaca ccaaaatcgt cgcgggcgca
    19561 cctgcacgtc tgttagcagc gggtatcggc gatgcgctgg caacctggtt tgaagcgcgt
    19621 gcttgctctc gtagcggcgc gaccaccatg gcgggcggca agtgcaccca ggcggcgctg
    19681 gcactggctg aactgtgcta caacaccctg ctggaagaag gcgaaaaagc gatgcttgcc
    19741 gccgaacagc atgtagtaac cccggcgctg gagcgcgtga ttgaagcgaa cacctatttg
    19801 agcggcgttg gttttgaaag cggcgggctg gcggcggctc acgcggtgca taacggcctg
    19861 accgctatcc cggacgcgca tcactattat cacggtgaaa aagtggcatt tggtacgctg
    19921 acgcagctgg ttctggaaaa cgcaccggtt gaggaaatcg aaaccgtagc tgcacttagc
    19981 catgcggtag gtttgccaat aactctcgct caactggata ttaaagaaga tgtcccggcg
    20041 aaaatgcgaa ttgtggcaga agcggcatgt gcagaaggtg aaaccattca caacatgcct
    20101 ggcggcgcga cgccagatca ggtttacgcc gctctgctgg tagccgacca gtacggtcag
    20161 cgtttcctgc aagagtggga ataacctact ccaaactccc ggcttgtccg ggagtttgaa
    20221 cgcaaaattg cctgatgcgc tacgtttatc aagcctacga aatctctgca atatattgaa
    20281 tatgcgtgct tttgtaggcc ggataaggcg ttcactccgc atccggcact ctgtgccagt
    20341 aatctgaaac tcccacgttg ttcaggagtt ttttatccgc ttctggacat tttctccaca
    20401 gaacaatcgt tagcccggaa ggtcgaatct gtgactctta ttttcacgga cgaaccagat
    20461 gaaagcttca ctggcgctgc tcagcctgct aacggcattt accagccact ccttaaaatc
    20521 tcccgccgtt ccaccaaccg tggtacaaat ccaggccaat accaaccttg ccatcgcaga
    20581 cggtgccaga cagcaaatcg gcagcacgct attttacgat ccggcgtata tgcagcttac
    20641 ctatcctggc ggtgatgtcc cgcaagaacg cggggtatgt tccgatgtgg tgatccgcgc
    20701 cctgcgcagc cagaaggtcg atttgcagaa actggttcac gaagatatgg cgaagaattt
    20761 tgccgagtac ccgcaaaaat ggaagttaaa gcgcccggac agcaacatcg atcaccgtcg
    20821 ggtgcctaat ctggaaacct ggtttagccg ccacaacaaa acacgcccca tcagtaaaaa
    20881 tgccggtgac tatcaagcgg gcgatattgt ctcctggcga ttagataacg ggctggcgca
    20941 tattggggtg gtgtcagatg gcttcgcccg cgacggtacg ccgctggtga tacataacat
    21001 tggcgcaggc gcgcaggaag aagatgtact gtttagctgg cggatggtgg ggcattatcg
    21061 gtattttgtt aaataagcga tcgccccaca tgaagaaaaa attcagatgc tttttacact
    21121 gacagcttct tttttcttac ggctcaccag cgccagtgcc agcacaatca gcacaatacc
    21181 gctaccttca accacgccag gattctcgcc gagcagccac caggaaaaga gtacgccgca
    21241 aacgggaacg gccagtgtgc ttaagctggc aatactggca ggcaagtttt tcaatacaaa
    21301 caaccataag ctccacgcca gcgccgtcgc cagaatcgca ctgtagacca gcgcccagaa
    21361 cacagtgggc tgccagtcaa tttcacgttg tggcaccagt aaagcgacca cactcatcac
    21421 cagcgccgca tacagcatct gccaggatgt taacgacaat aaatccacgc gcggatgacg
    21481 ggcatacaga cgtttagcaa caatcgcgct cgccccccaa ctaacaccgg agagaattgc
    21541 cagcatggca cttttcatcg aagagaaatc gagttgccac ggctgcaaca ccaaaaataa
    21601 gccgaaagcg gcaatcagta tcgcgaaata ttgcccacgt cgcaggcgtt caccgagaaa
    21661 caacgcggcg aaaatcacca cccagaacgg catggtatag ctcaggatcg ccaccttccc
    21721 cgcacctccg ctgaccaacg cccattgtgc caggccaatc attccgcagg tttgtaacag
    21781 ggcaatagct aaggtgtatt taaacggcgt cgggcgcatt gcgcgaccac gtaataaaag
    21841 gacgatgaat aaaacgagag cgccgaaaat gcagcgtaag gcggtgaagt cgaaggcacc
    21901 gatgtaactg gtgacttgct tcatgaaaat ccagctataa ctccagataa gtgtgagcac
    21961 aaccaggcca ctgattgcca gtgggttgct ctttcctgcg gcagacatga gagatccagt
    22021 gtgtagtttt cctgtggaca ctatacgccg gtaaagaaac ttcagcgact gcctgaaaga
    22081 cagtcgctga agaaggtcag attacagcag gtcgaaacgg tcgaggttca tcactttcac
    22141 ccatgccgcc acgaagtctt taacaaactt ctcgtgggca tcgctgctgg cgtaaacttc
    22201 cgctaccgca cgcaggacag agttagaacc aaataccaga tccgcacggc tggctgtgta
    22261 tttcacttcg ccggtttcac ggtcacgacc ttcgaacagc tctttcgatt cgtcagtcgc
    22321 tttccactcg taacgcatat ccagcaagtt cacgaagaag tcattgctca atacgccaac
    22381 gcggtcagtg aagacgccgt ttttgctgcc atcgaagttg gcacccagta cacgcatccc
    22441 gcccaccagc gcagtcattt ccggcgcggt cagcgtcagt tgctgtgctt tgtcgatcag
    22501 cagtgactcg gtggtggaaa cgtccagacg agcgcgatag ttacggaaac cgtcagcaat
    22561 tggctccagc agctcaaaca tctcaatgtc agtctgatcc tgacgcgcat caacgcgacc
    22621 cggcgcaaac ggtacatgaa tgctcaaacc tgcggcgctt gcggctttct caacaccaac
    22681 cacaccagcc agcacgatga tatccgccag cgaggcttta ccagactctt tctggatttt
    22741 ctccagaaca ggcagagcac gaacggctgc ggcgttcaca tcccagtcgc gctgcggcat
    22801 taatgccaga cgcgcaccgt tggcaccacc gcgtttgtcg ccaccacgga aggtagaagc
    22861 agatgcccag gccaccgata ccagctcact aacagacaga ccagaatccg caatcgcgaa
    22921 tttcagatcg ataatgtcct gctcggtcgg gttgtagatc ggctgcggca gcggatcttg
    22981 ccagatcaga tcttctttcg gtacttccgg tccgatgtaa cgagatttcg gccccatatc
    23041 cctgtgcgtc agtttgaacc aggcacgggc aaaggcttcg ttgaacgcct gcggatcgtt
    23101 gaggaaacga cgagagatct tctcgaactc aggatcaaaa cgcagcgtca ggtcggtcac
    23161 cagcatcgtc ggtttacgtt tcttcgacgg atcgaacgga tccgggataa tttccggtgc
    23221 gtctaccgct tcgaactgga ttgcgccagc cggactgcgg gtctgtaccc actcatattt
    23281 gaacaggttc tcgaagaaat agttgctcca ctgggtcggc gtctgggtcc agacaacttc
    23341 cagaccagag gtaatggcat ctgcgccaac gccgctgccg taagtgctcg cccagcctaa
    23401 accttgttct tcaatcggtg cagcttctgg atcaggacct acatttgatg tcggaccggc
    23461 accgtgggtt ttacccagcg tatgaccacc cgcaatcagc gccacggttt cttcgtcgtt
    23521 catgcccatg ttgccgaagg tcgcgcggat agctgctgcc gcagaaagcg gttcgccgct
    23581 gtggtccggg ccttccgggt taacgtaaat caggcccatc tcggttgcac ccagcggtgc
    23641 tttcgccagc gcttccggat gacggtgagt cagccaggct ttttcatcac cccagttaac
    23701 atccagatcc ggttcccaga cgtcttcacg accggcacca aaaccgaagg tacggaagcc
    23761 ggagttttcc agcgccacgt tacctgcgag gataaacagg tcggcccagg agattttctg
    23821 accatatttc tgtttgattg gccacaacag gcgacgcgct ttatcgaggc ttacgttatc
    23881 cggccaggag ttcagcggtg caaaacgttg ctgaccacga cccgcgccgc cgcgtccatc
    23941 gattgaacgg taagtacccg cgccgtgcca ggccatacga ataaacagac cggcgtaact
    24001 gccccagtcg gcaggccacc acggctgaga ttctgtcagc agggctttca gatctttttt
    24061 caggccgtag taatctaatt tgctgaattc tttgcggtag tcaaagtcct cacccagtgg
    24121 gttagaacga ttagaatgtt ggtttaacag gtcaacacgg agttgatttg gccaccagtc
    24181 gcgagtggtt gtgcccgccc ccgcgctctg gtcgtgaccg ccctgatgga acgggcattt
    24241 gccagtggct gtggtgttat gaatatcgtc tgacgtgctc atcaatgtgc tcccctctac
    24301 agtgttaccg ttacgataca cagcgttaga gagaagttat aattgaatta atccacggat
    24361 gcgatagttg agatcttaca tattgttggt taaagagatg tagatcaaat tgatcgtaat
    24421 taagcttttt tgattatgtc cgaattcgga caaatgcttt ataaaaaagg gttttaggtt
    24481 catatccttc aagtaatgag ggagaaaaca aggctgataa ccttattttc ccgccctcat
    24541 ttcgaggcag cattttgtgc tctgtttaaa atttgtgatc actgtgtgat tttcacaaaa
    24601 gccacactat ttataaacca ggtcgaacac ccagcgtatg gcaaatcgcg taactcattt
    24661 cagcgcggtt aagcgtatag aagtggaaat ctttcactcc ttcacggctt aaaatcttca
    24721 ccatatccat ggcaatattc gctcccacca gtttgcgggt ttcagcatcg tcatccagcc
    24781 cgtcaaacat ttgtgccatc cacgccggaa tacgcacgtt ggtcatatcg gcaaatttct
    24841 tcgcctgttt aaagttagat accggcaaaa tccccggaat aatttccaca tcaatgcccg
    24901 ccgatacgca gcggtcacga aaacgcaggt agctttcgac atcaaagaag aactgagtaa
    24961 tcgcgcggtt ggctccggca tcgactttgc gtttcaggtt gagcaaatcc gcctgagcgc
    25021 tttttgcttc cgggtgaact tccggatacg ccgccacgga gatatcgaaa tctgccactt
    25081 cttttaacag cgtcaccagg tcagaagcat acatttccgg cttaccactt cccggcggca
    25141 gatcgccacg cagcgccacg atatgacgaa taccgttatt ccagtagtcg cgtgcaatgg
    25201 tgcgcagctc gtcgggcgtc gcatcaatgc aagtaagatg cggtgccgct tccagaccag
    25261 tgcgatcttt aatgccttta ataatgctgt gcgtacggtc gcgctcgccg gagttcgcgc
    25321 cataggtcac cgatacaaac ttcggtttca ggctgctaag gcgatcgatg gagttccaca
    25381 gggtctgctc catttcactg gtacgcggcg ggaaaaactc gaacgaaacg ttaatctgcc
    25441 cctggacttc tgccaggctc tgattcaggg catcccgctg gctggcgtga aaaaagctca
    25501 taccttacct catcaatcgc ttgtagttat atgttgtgtt gtgcacatct atccatttag
    25561 acgtccagat gtaaagatga aggaaaagcc gaagggcgtc aaccgaaaaa tgaccaatga
    25621 tggtgaggaa aaatcaggga agatgaaaaa acttcaggat ggtaagaaaa agaaaatccg
    25681 gtatgcaaag caaaccggac tgcaaatata aactaaccat gtacttattg cttcttgccg
    25741 gattcgtcgt aaacgcttca tccggcctaa tcctatgata attcaatata tttcattaga
    25801 aaccatagac ccgaaaacat gagtaccggg cattattaaa tttctgaaat tacaacaact
    25861 gtgccagccg gttgatatcc gactgaatcg ccccggcggt gacgtcgcgc ccagcgccag
    25921 gcccacggat caccagcgga ttatcgcggt accagcggct ttcgatggca aagacgttat
    25981 cgcatggcag cagtgatgcc agcggatggt cttcacgcac cgcttccacg cccacacgcg
    26041 ctttgccatt ggcatcgaaa cgcgccacgt agcgcagcac cagccccatt tcgcgggccg
    26101 cttccagccg ttgcaccatc tgctcgttaa gttcatcgcc attttcaaag aaatggtcga
    26161 tgctgccgcc ttcgcaatga gcaggcacca gcgattccac acgtacctgg tccggttcaa
    26221 tgttgtaacc cgcttcacgc gccagaatta ccagcttgcg cattacgtct ttgccggaga
    26281 ggtcgtcacg cggatcaggc tcggttaagc cctgctgcca cgcctgatcc accagctcgg
    26341 taaacggcac gctaccgtcg aattgcagga acagccagga gagcgtgccg gagaagatcc
    26401 cgctgatcga caaaatagta tcgccgctgt cgatcagatc gcgcacggtg tggttgatcg
    26461 gcaagcccgc accgacggtg gcattgtaca gccagtgacg cccggttttt tcgaaggcgt
    26521 cgtggatctg gcgatattta ttgctgtcgc tggctcccgc cagtttgttg gcgctgataa
    26581 cgtggaaacc gtggctggcg aaatcaagat actgatcagc aagctgctgg ctggcggtaa
    26641 cgtccagcac cactaaatca tcatacggat gggcgcgcat ccacaggaac aacgactctt
    26701 catcctgctc aaccgcttca tcgttgaaga aggctaacgc gcggctggcg tccagcccgt
    26761 catagctcaa caggctgcgg cggctgtcca ccacacctgc cagcacaaac tcaaagccgg
    26821 tacgtgccga aagcgtgctc tgctcacggg cgaacagttc cagccaacgg gaaccgatat
    26881 tgcccttacc gaacaatacc aggccgatgc gtttttctgc gcggaagacg gactgatgca
    26941 gcccctgaat caggctttcg gtcgggccgg tgcgcagtac tgccaccagg ctgatgccgt
    27001 catcggactg ccaggtaaat tcgaccggct ggcctttcag ttgctgccag aagcggtggc
    27061 aatgcagcgg gttacgggtg acgcctgcac cgaccatcgc caccagcgcc agcccctgac
    27121 gcaggcgcag ttcgccaggt aatcccgctt cgtcgaggat tttcagcgca ctgtcggcca
    27181 cttctgaggt gtagcaaaat tgcagcaact ggcgatcgtt atgtacgcca accgccagcg
    27241 ggcgtacctg cgcgcgtttc aggatttggt cgatctcttt atgcgccagt ttgaaatcct
    27301 gactggcggg cacctgaaac tcaatcaaac agacatcatc gtggctggtg acaatacgcg
    27361 caccagtacc ggaggccagc acgcgttcaa tgcgcgtgga accttgatcc ggcgtgtagc
    27421 tacagcgcag ttgcaggtcg atttcgctgc cagaaaccgg ctgtaaagta cgggcgtgaa
    27481 gaacgggagc cgccaggcgc gccagttcgc tggcctcatc cagacgcagc aacggcagca
    27541 ggcaggcatc tttcacttta cgcgggtcgg cactgtatac cccggcgacg tcgctccaga
    27601 tggttacgcg agaaacaccc gccagcgcac cgatttgtgt cgcggaatag tcggaaccgt
    27661 tacgccccag cagcaccgtt tcaccggcgt tgttgcggct gataaatccg gtcaccacca
    27721 gacgtttgcc cggatgttgc accagcagct gttgcagcaa cgggtaagaa agcccttcat
    27781 caacctgcgg ttgtgcggcg cgttcagcgc gtaaaaactc gcgggcatca agccaggccg
    27841 ctggcagccc ttgttgatta agtaccgcag acatcaggcg tgccgaccac acttcaccgt
    27901 gccccaccac ttccgcatac actgcgtcgt taataccgct gtcgagcagc gccgccaggc
    27961 gctcaaggtc gctgacaaaa gcgctaatga ggctatcggc ttcttcagcg ggtagcagac
    28021 cgctaatcag atcgcactga tagcgacgca gcgtttgttg aacctgatgc gcagagagac
    28081 gatcggtctg gcttagtttc aaccagttaa tcaactggtt agtggtgcta ccggcggcgg
    28141 aaaccaccat catatcgtca ggctgagagt actccgccat aatgcccgcg acacgcaaat
    28201 aacacttcac atcagccaga ctactgccac caaatttatg cagctgacga cctttcgccc
    28261 ctgcctgcgc aatcacactc atttttaccc cttgtttgca gcccggaagc cattttccag
    28321 gtcggcaatt aaatcttcgc catcttcaat accggtggag atacgcagca gcgtctcgga
    28381 gatcccggcg gcagcacgcg cttctggtgc catgcctgca tgtgtcatgg ttgcggcgtg
    28441 agagattaaa ctttccactc cccctaatga ttccgccagc gtaaacaacg acagcccgct
    28501 caggaaacga cgcagcgtct gctcatcgcc atccagttca aaactcaaca ttgcgccaaa
    28561 gcctttttgc tgacgcgcgg caatttcatg cccctgattt tccggcagcg acgggtgata
    28621 cagttttttc accaacggct gggtttgcag atatttcaca atcgcctgcg cgttacgctg
    28681 ggccaactcc atgcgcggca ccagcgttcg caacccgcgt agtagcagat agctgtcaaa
    28741 cgcgccgccc gtcacgccaa tattgtttgc ccaccaggca agttcagtga caacgtccgg
    28801 gtctttagca atcaccacgc cggccaccac gtctgagtga ccgttcagat atttcgtgca
    28861 tgaatgcaac accagatcgg cacctaatgc cagcggattt tgtaatgccg ggcttaagaa
    28921 ggtgttatcc accacgctca ccgccccgac ttcccttgcc agatggcaga ttttcgcaat
    28981 atccacgacg cgtaacaatg gattacttgg gctttctacc agtaccagtt tgggtttttc
    29041 tgccagcgct gcccgtaatg cctgttcatc gccttgatca acaaacaaca cgcgatagca
    29101 accacgtttc gccaggctgt cgaacaggcg atagctaccg ccgtagcagt cgtgcggcgc
    29161 taccagcaga tcgccaggtt tcaaaaagac ggtcgttacc aggtgaatcg cggacatgcc
    29221 ggtattagta agtactgcac cagcaccgcc ttccagttct gccagtgctc gctgaaccac
    29281 atcgcgcgtt gggttgccac gacgcgagta atcatgcgcg cgcggttcat taaatccggt
    29341 aaagttatag gtactggaaa gatggatcgg tgggacaacg caaccatact gttcgtcgtc
    29401 atttaacccg ctacgcactg cgatggtggc ctgtttacgc gtcatgtgat gaagttccct
    29461 gggcttggtc ggtgaaatgt caggcaccag agtaaacatt gtgttaatgg acgtcaatac
    29521 atctagacat ctaaacttct ttgcgtatag attgagcaaa tcccaaatag ccgttaaaat
    29581 tatatgcatt atcacgccga caggtgcatt acacgatgtc acggtaacgc ctgtacggta
    29641 aactatgcgg gtttacggtc agtacccaca taaattgtgt ggtctggtct caatttattg
    29701 acgaagagga ttaagtatct catggctgaa tggagcggcg aatatatcag cccatacgct
    29761 gagcacggca agaagagtga acaagtcaaa aagattacgg tttccattcc tcttaaggtg
    29821 ttaaaaatcc tcaccgatga acgcacgcgt cgtcaggtga acaacctgcg tcacgctacc
    29881 aacagcgagc tgctgtgcga agcgtttctg catgccttta ccgggcaacc tctgccggat
    29941 gatgccgatc tgcgtaaaga gcgcagcgac gaaatcccgg aagcggcaaa agagatcatg
    30001 cgtgagatgg ggattaaccc ggagacgtgg gaatactaaa gcaaaaaaga gcggcgcgga
    30061 gtggaatcgc ctgatgcgct acgcttatca ggcctacgtc atattgcaat ttattgaatt
    30121 tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgagcac
    30181 gttgtctgcg acccaccgct ttttatacat ggacgtttaa ctatgaaaaa caggctgctg
    30241 atcctcagcc tgctggtttc tgtacctgcc tttgcctggc agccgcaaac cggcgacatc
    30301 atctttcaga tctctcgctc atcgcaaagt aaagcgatcc aactggcgac ccattccgat
    30361 tacagccaca ccggtatgct ggtgatacgc aacaaaaagc cctacgtttt tgaagcggtc
    30421 ggcccggtaa aatacacccc gctcaagcag tggatcgccc atggtgaaaa gggtaagtac
    30481 gttgttcgcc gcgttgaagg cggactgagt gttgaacaac agcaaaaact ggcgcaaacg
    30541 gcaaaacgtt atctcggtaa accgtatgat ttcagcttct catggagcga cgatcgccag
    30601 tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc tgggaatgcg cgtgggcgag
    30661 cagcagaagt tgaaagagtt cgatctcagc aacccgctgg tccaggcgaa gctcaaagaa
    30721 cgctacggca aaaatattcc gcttgaggaa acggtagttt caccacaagc cgttttcgac
    30781 gcgccacaac tcaccacggt tgccaaagag tggccgctgt tttcgtggta atggatgatg
    30841 aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta gttgcacgac ctttccgcaa
    30901 gctgtcgctc cggtaaacgg tgatgcctcc aattagttga attaaggata taatactcgc
    30961 ttttaaggtt gtttcatggt cacagttaac cttcattgcc cccgttgcca gtccgttcag
    31021 gtttaccgtc acgggcagaa cccaaaaaga catgatcgat tccgcggccg tgactgtcac
    31081 cgggtatttc agctcagtta ctgttacgag ccccgtaaac cgggagttaa agaccaaatc
    31141 gctgaaatga ctttcaacgg tgccggggtt cgcgatactg cccgtacgct gaaaatcggc
    31201 attaatacag tcatccgaac tttaaaaaac tctcgccacg aagaataacg tcatccccgg
    31261 tcgctcatgc tgatgtggca ctcatctgca aacttagtca gcaatggagc tttgtcggca
    31321 gtaaagcgcg acagcactgg ctctggtacg tgtacaacac gaaaactggt ggtgttctgg
    31381 cctacacttt tggcccccgg gctgacgaaa cctgtcgtga attgcgggcg ctgcttaccc
    31441 ttttaccatc ggcatgttga ccactgacga atggggtagt tacgctagag agttaccgaa
    31501 ggagaagcat ctgactggta agatttttac tcagcacatt gagcgtaaca atctgaccct
    31561 gagaacccga atcaaacgtc tggcccgcag aaccatctgt ttctcgcgtt ctgtagaact
    31621 tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt tctactaatt ggagtcatca
    31681 cccttctgat aagctttcct tggatgatta ccagactgaa tacacagcca aagattttta
    31741 cctcattctg ctcttttttc tcaaatgaca tatattatat tttacatcat attcatag
//
LOCUS       DAPHJE010000048        31405 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPHJE010000048 DAPHJE010000000
VERSION     DAPHJE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.048"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="RH222_002206"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="RH222_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3797220.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="RH222_002207"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="RH222_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV3797221.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="RH222_002208"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="RH222_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV3797222.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="RH222_002209"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="RH222_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV3797223.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="RH222_002210"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="RH222_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV3797224.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="RH222_002211"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="RH222_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV3797225.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="RH222_002212"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="RH222_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV3797226.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="RH222_002213"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="RH222_002213"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV3797227.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="RH222_002214"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="RH222_002214"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV3797228.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13246..13785
                     /locus_tag="RH222_002215"
     CDS             13246..13785
                     /locus_tag="RH222_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDV3797229.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14195..15328)
                     /gene="thiH"
                     /locus_tag="RH222_002216"
     CDS             complement(14195..15328)
                     /gene="thiH"
                     /locus_tag="RH222_002216"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV3797230.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15325..16095)
                     /gene="thiG"
                     /locus_tag="RH222_002217"
     CDS             complement(15325..16095)
                     /gene="thiG"
                     /locus_tag="RH222_002217"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV3797231.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16097..16297)
                     /gene="thiS"
                     /locus_tag="RH222_002218"
     CDS             complement(16097..16297)
                     /gene="thiS"
                     /locus_tag="RH222_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV3797232.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16281..17036)
                     /gene="thiF"
                     /locus_tag="RH222_002219"
     CDS             complement(16281..17036)
                     /gene="thiF"
                     /locus_tag="RH222_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV3797233.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17029..17664)
                     /gene="thiE"
                     /locus_tag="RH222_002220"
     CDS             complement(17029..17664)
                     /gene="thiE"
                     /locus_tag="RH222_002220"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV3797234.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17664..19559)
                     /gene="thiC"
                     /locus_tag="RH222_002221"
     CDS             complement(17664..19559)
                     /gene="thiC"
                     /locus_tag="RH222_002221"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV3797235.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19640..19740)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19792..20268)
                     /gene="rsd"
                     /locus_tag="RH222_002222"
     CDS             complement(19792..20268)
                     /gene="rsd"
                     /locus_tag="RH222_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV3797236.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20363..21136
                     /gene="nudC"
                     /locus_tag="RH222_002223"
     CDS             20363..21136
                     /gene="nudC"
                     /locus_tag="RH222_002223"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV3797237.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21176..22240
                     /gene="hemE"
                     /locus_tag="RH222_002224"
     CDS             21176..22240
                     /gene="hemE"
                     /locus_tag="RH222_002224"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV3797238.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22250..22921
                     /gene="nfi"
                     /locus_tag="RH222_002225"
     CDS             22250..22921
                     /gene="nfi"
                     /locus_tag="RH222_002225"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV3797239.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22964..23554
                     /locus_tag="RH222_002226"
     CDS             22964..23554
                     /locus_tag="RH222_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV3797240.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23741..24013
                     /gene="hupA"
                     /locus_tag="RH222_002227"
     CDS             23741..24013
                     /gene="hupA"
                     /locus_tag="RH222_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV3797241.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24026..24721
                     /locus_tag="RH222_002228"
     CDS             24026..24721
                     /locus_tag="RH222_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV3797242.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24723..25148)
                     /gene="zraP"
                     /locus_tag="RH222_002229"
     CDS             complement(24723..25148)
                     /gene="zraP"
                     /locus_tag="RH222_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV3797243.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25386..26762
                     /gene="zraS"
                     /locus_tag="RH222_002230"
     CDS             25386..26762
                     /gene="zraS"
                     /locus_tag="RH222_002230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV3797244.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26759..28084
                     /gene="zraR"
                     /locus_tag="RH222_002231"
     CDS             26759..28084
                     /gene="zraR"
                     /locus_tag="RH222_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV3797245.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28081..29370)
                     /gene="purD"
                     /locus_tag="RH222_002232"
     CDS             complement(28081..29370)
                     /gene="purD"
                     /locus_tag="RH222_002232"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV3797246.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29382..30971)
                     /gene="purH"
                     /locus_tag="RH222_002233"
     CDS             complement(29382..30971)
                     /gene="purH"
                     /locus_tag="RH222_002233"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV3797247.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7418 a         8127 c         8586 g         7274 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtcg aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggt
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cgttgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacctggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agactccgcc ggcagcagtt ctgctgaaaa aagcggctgg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgcgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgc ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccag gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgta tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat cctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg ggccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat cgacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcacc
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacatcgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 gggttaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gggcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctagtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggta tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgtgatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcgaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag ggtggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatatcctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtta aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca agggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaatactg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggtca
    11941 gaccattact cgtcagacag acgaattgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacaccc tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagca cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgttaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggggcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atggggggag tttagggaaa gagcatttgt cagaatattt aaggaatttc tgaatactca
    13141 taatcaatgt agagatgact aatatcctga aactgactga actaattgag tcaaactcgg
    13201 caaggattcg atactattcc tgtgtaactt tcttaaggaa cgagaatgaa acaggaagtg
    13261 gaaaagtggc gaccttttgg acatccggat ggtgatattc gtgatttatc atttcttgat
    13321 gctcatcagg ctgtctacgt tcagcatcat gagggcaaag agcctttaga gtatcgcttt
    13381 tgggttacct actctcttca ctgcttcaca aaagattatg aacatcagac gaacgaagaa
    13441 aaacaatcgt taatgtacca cgcgcctaaa gaatctcgtc ccttctgcca gcaccgttat
    13501 aacttagcgc gcacacactt aaaaagaact attttggcgc tgccagaaag caacgttatt
    13561 catgccgggt atggtagcta tgccgtgatt gaggtggact tagacggagg agataaggca
    13621 ttttactttg ttgcgttcag ggctttcagg gaaaagaaaa aactccgttt gcatgtaact
    13681 agcgcttatc ccatttctga aaaacagaaa ggtaaatcag tgaaattttt caccattgcc
    13741 tacaacttat tgagaaataa gcagcttcct cagccctcaa aataacaaaa cccaccttaa
    13801 ggtgggtttc gccagagaat tatctctggt attcagaacg ccattaccgg actttgcctt
    13861 gaccttgcga taatcgcagg ttgcgggatg tctgaatttc ttcagtctgc tgcatcctgg
    13921 aagatgagaa catgtgttct tattttcgtc tctatcatag ttgagtattt actctcttac
    13981 aatcagatct ctttcattgc tcaacaggcg atggcttcag actttgcatt acggaatttt
    14041 taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat tcctgctgac
    14101 ggcactacca taaaaaaaca gaacgctttc aacaaccttg cgtttttcat cttccgtagc
    14161 ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag gcccgtccca
    14221 gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac gcggcagcaa
    14281 ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg tgattatcgg
    14341 catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc agcggaataa
    14401 cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc tccggtgcaa
    14461 gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc gacgcaggct
    14521 caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt
    14581 aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg cgccagttgt
    14641 cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc cccagccgat
    14701 ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcagatga tggcgggcat
    14761 aagtcgcctc gtgatatgtc tcctgataaa ccatcacgcc atccagacca agctgcttta
    14821 actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa gagaactgtt
    14881 cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc tgatgttcac
    14941 cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt tccctggcaa
    15001 tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat ccgcagtacg
    15061 tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta actgtgttgc
    15121 caaatcgctg acgggtcaga cgctgcgccc gttgggccag ttgttccaga tagccactgg
    15181 cggctggcga taacagcgcc atcatatcgt cgcgggtgaa ttgcgaggca tttagcgccc
    15241 gctctacgtc aacagccgtt ttgccgttga tacgcaggcg gatgtcatcc cagtccagtt
    15301 gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccag tcagcgggct
    15361 ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc
    15421 ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcta cggcaatcgc
    15481 cgtattcact aacaccgcgt cggcccccat ttccagggcc tgcgcggcat ggctgggaac
    15541 gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa tctccagcat
    15601 ggcacaggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc
    15661 acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt aaggcagcac
    15721 gacaaatccc tgttgtacca gcatttcggc ggctttcaga gtttcgatgg gatcgggcaa
    15781 cagccagcgg gcgtcagggt gaatctccaa ttttagccag ttagtgccta acgcttcacg
    15841 tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggatg tatttggcag
    15901 cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc
    15961 gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg cctccaccat
    16021 cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc
    16081 cgcaatacgt aacatttcaa ccccctgcaa taacctgaaa aagcaggatc tggtcgccat
    16141 cctgcacgat atgttgcgcc cactgctcac gcgggacgat ttgctgatta atcgccagag
    16201 ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg
    16261 cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca taccgggcaa
    16321 ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc gaacagtcgg
    16381 agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag tgcctgcaaa
    16441 gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt gcgttctggc
    16501 tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg cgtcagtacc
    16561 atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg
    16621 caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag caccacatcg
    16681 gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa tgctgtcagt
    16741 tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt cgggcgatcg
    16801 atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca
    16861 tctgccagta ccagcgtccc gacgccagcg cccgccaggt acagcgcagc aggtgtaccc
    16921 agcccgccca gaccgataat cagcacctgg ctgtcgagca gtttttgctg cccgtccaga
    16981 gcgatatcgt cgagcaggat ttggcggcta taacgcataa agtcacggtc attcatcgcc
    17041 aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcag cttgagtaat
    17101 ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg cgcgtgccag
    17161 actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat gccgtgccag
    17221 ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc
    17281 cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt
    17341 cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc
    17401 ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc agtaatcgtt
    17461 gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga catcggcttc
    17521 cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa
    17581 cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac gaaaaggtac
    17641 aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat ttctccgcct
    17701 ctggcacgga agttctccga catatccgcc attcccattt caatagtttg cgtggcggcg
    17761 taatcacgca cttcctggct gattttcatc gagcaaaatt tcggtccaca catggagcaa
    17821 aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc gcgggcggta
    17881 aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg ggctttcgac
    17941 atggcgttat cgcgaatttg cgcgcccgga tgccctttcg ccaggtcagc ggcgtgggcg
    18001 gcaatcttat aggtgataag cccctgctta acatcttctt tattaggcag acccagatgc
    18061 tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat cgccgcacca
    18121 atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg ccccagagtg
    18181 taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg gatcatctgc
    18241 atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca ggcaattttg
    18301 gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc gtcctgaata
    18361 gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc acagatttca
    18421 caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag gcaccatttc
    18481 gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt catcggcaca
    18541 tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc ggcctgttcc
    18601 agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt aaccttctcc
    18661 agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg
    18721 cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc tccccagcgc
    18781 gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc cgagttgccg
    18841 atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc cgattccgga
    18901 tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc
    18961 gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa
    19021 acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa ttccatttcc
    19081 ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc tgctttggcg
    19141 cgttttggtg ttagtacgcc gctaaaacgc agttcgtcga ggccatcatc tgccagccgc
    19201 gctttagtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc
    19261 cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcaatctg cggatcacca
    19321 tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg cggctgttct
    19381 ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac gcgcacgccg
    19441 gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg
    19501 tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata
    19561 gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca agagacgatc
    19621 gccgcagggg cgatgcgata gcagattaac tcttgttccc ttcgcaggta ttagcctgat
    19681 caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg acaagaaatt
    19741 agccccgcaa atggggcatt gaatgtaaat tacgcgttaa cagcgcagaa ctcaagcagg
    19801 atgtttgacg cgggcggcgt caagcaccag cagaatcagc ttatcttcca gcacaaagcg
    19861 cgcttccagc gcctcgccga tgtcagataa aacctgttga aactcaaggt aattatcatg
    19921 atcgatggcg gtttccagac tggaatcgta gtaatccata atctgttgag tattggcttc
    19981 gagttgcggc caaatcttcg cggcgcgcgc gagttgcccg ttgccttcca gcttatgaag
    20041 aatgcgctca taaatactga aatgtccggc agacaagtaa tcgaccaggc tctgacaaaa
    20101 atcatcaagg gctttttcgt ttagcctcat gtacgattct ttgccaggct taatgccaac
    20161 cagattgtag taagccacga gcagatgctt acgtacatgt agccagcgat caaccagttt
    20221 gttacttcct ctgacgcgct ccgtcaggtt atcgagctgg ttaagcatga ttgactccgc
    20281 aagtttgtat tcaaaaactg ctcagtgaga aatgtaaaaa ccatgttaaa catgccagtg
    20341 atgcaaaggt agtgcaagag ctatggatcg tataattgaa aaattagatc acggctggtg
    20401 ggtcgtcagc catgaacaaa aattatggtt gccgaaggga gaattgccat atggcgaagc
    20461 ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc ggcgaatggc agggggaacc
    20521 tgtttggtta gtacaacagc agcggcgtca cgatatgggg tcggtacgtc aggtcattga
    20581 tctcgatgtt gggctgtttc aactggccgg acgaggcgta caactggcgg agttttaccg
    20641 atcgcataaa tactgtggtt actgcgggca tgaaatgtat ccgagcaaaa ccgaatgggc
    20701 gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa atcgccccct gcattattgt
    20761 tgccatccgt cgcgatgatt cgatcctcct cgcccagcat acccgccatc gtaacggtgt
    20821 ccatacagta cttgccggat tcgtcgaagt gggcgaaacc ctcgagcagg cagtcgcgcg
    20881 ggaagtgatg gaagagagcg gaattaaagt taaaaacttg cgttacgtga cttctcagcc
    20941 gtggccgttt cctcagtctt taatgaccgc gtttatggcg gaatatgaca gcggcgacat
    21001 cgtgatcgac ccgaaagaat tgctcgaggc gaactggtat cgctatgacg atttgccgtt
    21061 actcccgccg cccggcaccg tagcgcgccg tctgatagaa gatacggtgg cgatgtgtcg
    21121 ggcagagtat gagtgatgat acactgaccg cctgacgcac taaggaacag ccaaaatgac
    21181 cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc cagcccgttg atgtcactcc
    21241 agtatggatg atgcgccagg cgggtcgcta tctaccggaa tataaagcca cgcgcgccca
    21301 ggcgggcgat tttatgtcgc tgtgcaaaaa cgccgagctg gcgtgcgaag tgactttgca
    21361 accgctgcgt cgctacccgc tggatgcggc gatcctcttt tccgatatcc tcaccgtgcc
    21421 ggacgcgatg gggttagggc tctattttga agccggagaa ggtccgcgtt ttacctcgcc
    21481 agtcacctgc aaagccgacg tcgataaact gccaattccg gacccggaag atgaactggg
    21541 ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg aaaggcgaag tgccgctgat
    21601 tggtttttcc ggcagcccgt ggacgctggc gacctacatg gtggaaggcg gcagcagcaa
    21661 agccttcacc gtgatcaaaa aaatgatgta tgccgatccg caggcgctgc acgctctgct
    21721 cgataaactg gcgaaaagcg tcactttgta tctgaatgcg cagattaaag ccggtgctca
    21781 ggcagtgatg attttcgaca cctggggcgg cgtgcttacc gggcgcgatt atcaacagtt
    21841 ctcgctctat tacatgcata aaattgttga tggtttagtg cgtgaaaacg acggtcgccg
    21901 cgtaccggtc acgctgttta ccaaaggcgg cggacagtgg ctggaagcca tggcagaaac
    21961 cggttgcgat gcgctgggcc tcgactggac aacggacatc gccgatgcgc gccgccgtgt
    22021 gggcaataaa gtcgcgttgc agggtaatat ggatccgtcg atgctgtacg cgccgcctgc
    22081 ccgcattgaa gaagaagtag cgactatact tgcaggtttc ggtcacggcg aaggtcatgt
    22141 ctttaacctt ggtcacggca ttcatcagga tgtgccgcca gaacatgctg gcgtgttcgt
    22201 ggaggcagtg catcgactgt ctgaacagta tcaccgctaa ggagtgatta tggatctcgc
    22261 gtcattacgc gctcaacaaa tcgaactggc ttcttctgtg atccgcgagg atcgactaga
    22321 taaagatcca ccggatctga tcgccggagc cgatgtcggg tttgagcagg gcggagaagt
    22381 gacgcgagcg gcgatggtgc tgctgaaata tccctcgctt gagctggtgg agtataaagt
    22441 tgcccgcatc gccaccacca tgccttacat tccaggtttt ctttccttcc gcgaatatcc
    22501 tgcgctgctg gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg
    22561 tcatgggatc tcgcatcctc gccgtcttgg cgtcgccagc cattttggct tattggtgga
    22621 tgtgccgacc attggcgtgg cgaaaaaacg tctctgcggt aaattcgaac cgctctccag
    22681 cgaaccgggc gcgctggccc cgctgatgga taaaggcgag cagctggcct gggtctggcg
    22741 cagcaaagcg cgctgtaacc cgttgtttat cgctaccggc catcgggtca gcgtggacag
    22801 cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt ctgccggagc caacgcgctg
    22861 ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa atcagcccta
    22921 attcaggtaa actgcggata atttccgtat ttgagaactc aacatgttac aaaacccaat
    22981 tcatctgcgt ctggagcgcc tggaaagctg gcagcatgtc actttcatgg cttgcttatg
    23041 cgaacgcatg taccccaatt acgccatgtt ctgccagcaa accggttttg gtgatgggca
    23101 aatttaccgt cgtattctcg atctcatctg ggaaacgctg accgttaaag acgcaaaagt
    23161 aaatttcgac agccaactgg agaaatttga agaagcgatt ccttcagccg acgatttcga
    23221 tctgtacggc gtttatccgg caatcgatgc ctgcgtggcg ttaagtgaac tggtccattc
    23281 gcgtttgagt ggtgaaacgc tcgaacacgc ggtggaagtg agtaagacct ccatcacgac
    23341 cgttgcgatg ctggaaatga ctcaggctgg tcgcgaaatg agcgatgaag agcttaaaga
    23401 aaacccagct gtagagcaag aatgggacat tcagtgggaa atattccgac ttttagctga
    23461 gtgcgaagaa cgcgatatcg agctgataaa aggccttagg gcagacctgc gtgaggcggg
    23521 tgagagcaat attggtataa tttttcagca ataagaccag aaaacgtgat ttaacgcctg
    23581 atttgtcgta cctggagtct tccctttcgc cccccgtctg gtctacattt ggggggcgaa
    23641 aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat tctggcattt ccgtcgcact
    23701 cgatgcttag caagcgataa acacattgta aggataactt atgaacaaga ctcaactgat
    23761 tgatgtaatt gcagagaaag cagaactgtc caaaacccag gctaaagctg ctctggagtc
    23821 cactctggct gcaattactg agtctctgaa agaaggcgat gctgtacaac tggttggttt
    23881 cggtaccttc aaagtgaacc accgcgctga gcgtactggc cgcaacccgc agaccggtaa
    23941 agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga
    24001 cgcagttaag taagattgcg tggcagtgaa cagttttaac gaaggggtgg tttcacccct
    24061 tttgtctttc tggcgtcgat cattgatgct ggcaggtgcg ctgtttctca ctgcctgtag
    24121 ccacaactct tcacttcctc cctttaccgc cagtggattt gctgaagacc agggcgcggt
    24181 acgcatctgg cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg
    24241 gcgcagtggc gataccacga cgcgagagta tcgctggcag ggcgataacc tcacgctcat
    24301 caatatcaat gtttacagca aaccgccggt gaacattcgc gcgcgttttg acgatcgcgg
    24361 tgatctgagc tttatgcaac gtgaatccga tggggaaaag cagcagcttt ctaacgacca
    24421 aatcgattta taccgttatc gtgctgatca gatccgccag attagcgatg ccttacgtca
    24481 ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg tgaccacctg
    24541 cgaagggcaa accattaaac ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg
    24601 ccagagccgc tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca
    24661 attactgtta gtggcaaact ctgatttctg tcgctggcaa cccaacgaga aaacgttctg
    24721 atttaccagt ggcccatacc catatgaccg ccaccaccgc agccgccgta gcccatgccc
    24781 attccggctc cgcgcggaat acccgcttca gccatcgcga tatctcgttt cacccgtaac
    24841 tcatctaacg actgacgcaa attctccatc tctttggcga ccgcgttaat tttgctgcta
    24901 tccggtgggt tcgcggctaa cagggcattg tattcataac gcttcgtcac cagttgctgt
    24961 tgcagtgcgc tgctttgagc gtaaaagtca ttatggattt tctgccacgc tgtctgttgt
    25021 tcgctggtca aaggcgcggc attttgctgc cacataccgt gtccgccgtg agcaaatgca
    25081 gatgtcgatc ccatcgccat tgctgaaagc gccatcatta ccagggcaat tttcgtgttc
    25141 cgtttcatgg ttaatcctcc agtggttgtc ttacttcggg tattgcatct tccgtgccaa
    25201 cgatgaaacg ctgatatgac gggtaatctg gcatgataaa cgagtaaaaa tgactcgcct
    25261 gctgcgggta gcgagtcatt tttactcatt gaaacttaag cctttgtgtt acagcgcagg
    25321 gtaagcgctg ataaaagatg gcatgatttc tgctgtcaga aagggatgag caggcaaaga
    25381 agaagatgcg ttttatgcaa cgttctaaag actccttagc taaatggtta agcgcgatcc
    25441 tccccgtggt cattgttggg ctggtagggt tgtttgcggt gacggtgatt cgcgattatg
    25501 ggcgcgagac tgccgccgcc agacaaacgc tgctggaaaa aggcagtgta cttattcgcg
    25561 ctcttgaatc cggctcgcgc gtcggcatgg ggatgcgcat gcatcatgcg cagcagcagg
    25621 ccttactgga agaaatggcc gggcagcctg gagtacgttg gtttgcggtc acggatgaac
    25681 aaggaacaat cgtgatgcat agcaactccg gcatggtggg aaaacagctt tattccccgc
    25741 aggaaatgca gcagttacat cctggagatg aagaagcgtg gcggcggatc gatagcgcag
    25801 acggcgagcc tgttctggaa atttatcgcc agtttcaacc gatgtttgct gctggaatgc
    25861 accggatgcg ccatatgcag cagtatgccg cgacaccaca agcaattttc atcgctttcg
    25921 acgccagtaa tattgtgagt gccgaagatc gtgagcagag aaacaccctg attatcctct
    25981 tcgccctggc gacggtcttg ctggcaagcg tattgtcatt cttctggtat cgccgctatc
    26041 tgcgctcgcg ccagcttcta caagatgaaa tgaagcgcaa agagaagctg gtggcgctgg
    26101 ggcatcttgc ggcaggcgtt gcccacgaaa tccgtaaccc actttcctcg attaaaggac
    26161 tggcgaaata ctttgccgaa cgcgcgccag cagggggaga agcgcatcaa ttggcgcagg
    26221 tgatggcgaa agaagccgac cgtttaaacc gcgtggtaag cgagttgctg gaactggtta
    26281 agccaacgca tctggctttg caggcggtgg atctcaacac gctgattaac cactcattac
    26341 agctggtaag ccaggatgca aacagccggg agatccagtt acgctttacc gccaacgaca
    26401 cattaccgga aattcaggcc gatccggaca ggctgactca ggtcctgttg aatctctatc
    26461 tcaatgctat tcaggcgatt ggtcagcatg gcgtgattag cgtgacggtc agcgaaagcg
    26521 gcgcgggcgt gaaaatcagc gttaccgaca gcggtaaggg aattgcggca gatcagcttg
    26581 aagccatctt cactccgtac ttcaccacca aagccgaagg caccggattg gggctggcgg
    26641 tcgtgcataa tattgttgaa caacacggtg gtacaattca ggtcgcaagc caggagggaa
    26701 aaggctcaac gttcaccctc tggcttccgg tcaatattac gcgtaaggac ccacaaggat
    26761 gacgcacgat aatatcgata ttctggtggt ggatgatgac attagccact gcactatttt
    26821 gcaggcttta ctgcgcggct ggggctataa cgtcgcgctg gcgaacagcg ggcgacaggc
    26881 gctggagcag gtgcgggaac aggtttttga tcttgtgctt tgcgatgtgc gaatggcgga
    26941 gatggacggc atcgccacgc tgaaagagat caaagcgtta aacccggcaa ttccggtgct
    27001 gattatgact gcgtactcca gcgtcgagac ggcggtagag gcactgaaaa ctggggcgct
    27061 ggattatctc atcaagccgc tggatttcga taacctacag gcgacgctgg aaaaagcgct
    27121 cgcgcatacg cacagtattg atgctgaaac acctgcggtg actgccagcc agttcggtat
    27181 ggtcggtaaa agcccggcga tgcaacacct gctcagtgaa atcgccctcg tcgcgccatc
    27241 ggaagccacg gtactgatcc acggcgattc cggcaccggt aaagagctgg tcgccagggc
    27301 gattcacgcc agtagcgcac gtagcgaaaa accgctggta acgctcaact gtgcggcact
    27361 caacgaatcc ttactggaat ctgaattgtt cggtcacgaa aaaggggcgt ttactggagc
    27421 cgataaacgc cgggaagggc gctttgttga ggcggacggc ggcacgctgt ttctcgatga
    27481 aattggcgat atctcgccga tgatgcaggt gcgtctgctg cgtgcgattc aggagcgcga
    27541 agttcagcgt gtcggtagca accagactat ctcggttgat gtccggctga ttgcggcgac
    27601 ccatcgcgat cttgccgcag aggtgaatgc cgggcgtttt cgccaggatc tctactatcg
    27661 cctgaatgtg gtggcgattg aagtaccatc gctgcggcaa cggcgggaag atatccctct
    27721 gctggctggc cattttctgc agcgctttgc cgagcgtaat cgcaaggcgg taaaaggttt
    27781 tacgccccag gcgatggatc tgttgattca ttacgactgg ccgggaaata ttcgtgagct
    27841 ggaaaacgcg gtggaacggg cagtggtgct gctgaccggg gaatatattt ccgaacgcga
    27901 gctgccgctg gcgattgcca gtacgccgat cccgctggga caaagtcagg atattcagcc
    27961 gttggtggaa gtggaaaaag aggtgattct ggcggcgctg gagaaaacgg gcggcaacaa
    28021 aaccgaagcc gcccgtcagt tagggatcac gcgcaaaacg ctattggcaa aactgtcgcg
    28081 ttagttctgc tcgcgttcga tagcgcgcca gccgatatct ttccggcaga agcagtcgtc
    28141 ccagtgaata tcggtcatta aggcataagc gcgtttctgc gcttctgcca cggtatgacc
    28201 cagcgcggtg acgcacagta cgcgcccgcc gctggtcact acctgctcgt catccgccag
    28261 ttttgtgcct gcatggaaca ctttgccgtc ttccacttct tccagcggca ggccgtggat
    28321 cacatcaccg gtgcggtaat ctcccggata tccacccgca gccatcacca cgccgagaga
    28381 agcgcgttca tcccactcgg atgttttctc gtccagcttc ccttcacagg ctgccaggca
    28441 gagctcaacc agatcggact tcatgcgcag cataatcggc tgggtttccg gatcgccaaa
    28501 gcggcagtta aattcgataa ccttcggatt gccctgtttg tcgatcatca ggcccgcgta
    28561 gagaaaaccg gtgtaggtgt tgccttccgc cgccatgcct ttcacggttg gccagatgat
    28621 acgttccatg gtgcgctgat gaacttcatc ggttactacc ggcgcggggg agtaagcgcc
    28681 catcccgccg gtgtttggtc cggtatcttt atcgcctacg cgtttgtgat cctggctggt
    28741 agccatcggc agcacatgct ctccgtccac catcacgata aagctcgctt cttcgccatc
    28801 gaggaactct tcgataacga tgcgatgacc cgcgtcgcca aaagcgttgc ccgccagcat
    28861 atcgtgaaca gccgcttccg cttcttccag cgtcatcgcc acgataacgc ctttcccggc
    28921 agccagaccg tccgctttaa tgacgattgg cgcgcctttc tcacgcagat acgccagcgc
    28981 aggttctacc tcggtgaagt tctggtattc cgccgtaggg atcttatggc gggccaggaa
    29041 atctttggta aacgcttttg agccttccag ttgggccgca cctgcggttg ggccgaagat
    29101 tttcagcccg gcggcgcgga aggtatcgac cacgcctttc accagcggcg cttccgggcc
    29161 gacgatggtc agatcaatct tttcgttttg tgcgaaatcc aacagcgccg ggatatcggt
    29221 tacgccgata gcgacgttct gtagcgcggg ttccagcgca gtgcctgcat tacccggagc
    29281 aacaaaaaca gtttcaacca gcggcgactg cgccgctttc caggccagcg cgtgctcgcg
    29341 cccgccgtta ccaatcacta atactttcat ctattgctcc attaatggcg gaagtggcgc
    29401 atgtcggtga agagcatcgc aataccgtgc tcgtcggcgg cggcaatcac ttcgtcatca
    29461 cggatagaac cgccaggctg gattacgcag gtcacgcccg cagcggcggc ggcatcaata
    29521 ccgtcgcgga acgggaagaa cgcgtcagaa gccatcgagg aacctttcac ttccaggcct
    29581 tcatcggccg ctttaatacc ggcgattttt gcggagtaca cgcggctcat ctggcccgcg
    29641 ccaatgccga tagtcatatt gtttttggca tagacgatag cgttggattt cacaaacttc
    29701 gccaccttcc agcagaacag cgcatcacgc agttcctgtt cgctcggctg acgtttggtc
    29761 accacgcgca gttcttccgc accgaccatc cccaggtcac gatcctgaac cagcagaccg
    29821 ccgttcacgc gtttgaaatc gaggcccgga acacgctcgc cccactgacc gcaggtcaga
    29881 acgcgtacgt tctgtttggc ggcggtgatt ttcagggctt cttcgctggc ggacggcgca
    29941 ataatcactt cgacaaactg acgagaaatg atggcctgtg cggtttccgc atccagctcg
    30001 cggttaaagg caatgatgcc gccgaatgcg gaggttgggt cggttttgta cgcgcgatcg
    30061 taagcatcaa gaatggaatt gccgatagcc acgccgcaag ggttggcgtg cttcacaatc
    30121 acacatgccg gctcggcgaa ctctttcacg cactccagcg ccgcatcggt atcggcgatg
    30181 ttgttataag agagggcttt accctgaacc tgggttgcgg tagcaacgga ggcttctttc
    30241 acattctctt ctatatagaa ggcagcctgc tggtggctgt tctcgccgta acgcatatcc
    30301 agcttcttaa tgaagttcag gttcagcgtg cgtgggaagc gaccggcggc ttctttgctt
    30361 tcaccgtggt aagccggaac catgctgccg aagtagttgg caatcatgct gtcgtaggcg
    30421 gcagtgtgtt cgaaggcttt gatggcgagg tcgaaacggg ttgcaagcgt cagcgatcct
    30481 tcgttgtcat ccatctcttt aataatggcg tcgtagtcgc tgctcttcac cacgattgcg
    30541 acatctttat ggttcttggc ggcggagcgc accatcgttg ggccgccgat atcgatgttc
    30601 tcaaccgcat cttccagcga gcaaccttca cgggccacgg tctgggcgaa cggatacagg
    30661 ttaacaacca ccatatcgat aggctggatc tgatgttctt ccataatggc atcgtcctgg
    30721 ccgcgacggc ccagaatgcc accatgtact ttcggatgca gggtcttcac gcgtccatcc
    30781 atcatctccg ggaaaccggt gtaatcggaa acttcggtta ccggcagacc tttttctgct
    30841 aacagacggg cagtgccccc tgtagacagc agctccacac cgcgtgcgga aagtgcctgg
    30901 gcgaattcga cgataccggc tttgtcagaa acactgagca gagcgcggcg gactggacga
    30961 cgttgttgca tggtaaatcc cctggatttg actattacag agagcgttag ctgaattttt
    31021 cgcgaaaaac tcagctaacg cccctaacgg ggcatcctta tttttcgccc gcattgtaac
    31081 gaaaacgttt gcgcaacgct cgcgaatttt tctctttcaa tggtgatcac aattttgaca
    31141 gtggttaccg tgggaaaaat acagaaatta cattgatgat tgtggataac tctgtgcgta
    31201 aaaaggtata aagcggggtt ttgctgggga atgcagcagt cagttatttt tctgcaattt
    31261 ttctattgcg gcctgcggag aactccctat aatgcgcctc catcgacacg gcggatgtga
    31321 atcacttcac acaaacagcc ggttcggttg aagagaaaaa tcctgaaatt catggttgac
    31381 tctgaaagag gaaagcgtaa tatac
//
LOCUS       DAPHJE010000049        31023 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPHJE010000049 DAPHJE010000000
VERSION     DAPHJE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.049"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            113..3865
                     /gene="yfaL"
                     /locus_tag="RH222_002234"
     CDS             113..3865
                     /gene="yfaL"
                     /locus_tag="RH222_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV3797248.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILHYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3993..4715)
                     /locus_tag="RH222_002235"
     CDS             complement(3993..4715)
                     /locus_tag="RH222_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV3797249.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4862..7489
                     /gene="gyrA"
                     /locus_tag="RH222_002236"
     CDS             4862..7489
                     /gene="gyrA"
                     /locus_tag="RH222_002236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV3797250.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7638..9326
                     /locus_tag="RH222_002237"
     CDS             7638..9326
                     /locus_tag="RH222_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV3797251.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKMFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9323..9946
                     /locus_tag="RH222_002238"
     CDS             9323..9946
                     /locus_tag="RH222_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV3797252.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGIASQYLPPEMTLKPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWILNDSNPNFIGIYRLNFLAR"
     gene            9970..14484
                     /locus_tag="RH222_002239"
     CDS             9970..14484
                     /locus_tag="RH222_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV3797253.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPFEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     IGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVRVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNVPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDPSDINDSLIFDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYARDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14485..16134
                     /locus_tag="RH222_002240"
     CDS             14485..16134
                     /locus_tag="RH222_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV3797254.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQKRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKKPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16139..16915
                     /locus_tag="RH222_002241"
     CDS             16139..16915
                     /locus_tag="RH222_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV3797255.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16989..18173)
                     /gene="atoB"
                     /locus_tag="RH222_002242"
     CDS             complement(16989..18173)
                     /gene="atoB"
                     /locus_tag="RH222_002242"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDV3797256.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18204..19526)
                     /locus_tag="RH222_002243"
     CDS             complement(18204..19526)
                     /locus_tag="RH222_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDV3797257.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19523..20173)
                     /gene="atoA"
                     /locus_tag="RH222_002244"
     CDS             complement(19523..20173)
                     /gene="atoA"
                     /locus_tag="RH222_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDV3797258.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20173..20835)
                     /gene="atoD"
                     /locus_tag="RH222_002245"
     CDS             complement(20173..20835)
                     /gene="atoD"
                     /locus_tag="RH222_002245"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDV3797259.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21031..22416)
                     /gene="atoC"
                     /locus_tag="RH222_002246"
     CDS             complement(21031..22416)
                     /gene="atoC"
                     /locus_tag="RH222_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDV3797260.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22413..24239)
                     /gene="atoS"
                     /locus_tag="RH222_002247"
     CDS             complement(22413..24239)
                     /gene="atoS"
                     /locus_tag="RH222_002247"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDV3797261.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24406..27255
                     /gene="rcsC"
                     /locus_tag="RH222_002248"
     CDS             24406..27255
                     /gene="rcsC"
                     /locus_tag="RH222_002248"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV3797262.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27455..28105)
                     /gene="rcsB"
                     /locus_tag="RH222_002249"
     CDS             complement(27455..28105)
                     /gene="rcsB"
                     /locus_tag="RH222_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV3797263.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28122..30794)
                     /gene="rcsD"
                     /locus_tag="RH222_002250"
     CDS             complement(28122..30794)
                     /gene="rcsD"
                     /locus_tag="RH222_002250"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV3797264.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEQDATATGNNDNEKEGTDSVSIHFNS
                     TKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7586 a         7658 c         8325 g         7454 t
ORIGIN      
        1 taataacaaa atagccattg caccgggttg aacaatttac aaaagaaaga tattccatat
       61 cgtataatgc gattaaatac gccgtcttat agaaaatgat ggttagatta atatgcggat
      121 tatctttcta cgcaaggagt atttatcttt actcccgtca atgattgcat ctcttttctc
      181 tgctaacggt gtcgcggcgg tcactgattc atgccaggga tatgatgtca aagcgagttg
      241 tcaggccagc aggcaaagcc tttcaggcat tacgcaggac tggagtatcg ctgatgggca
      301 atggctggtt ttttcggata tgaccaataa cgccagcggt ggggccgtat ttttgcaaca
      361 aggagcggaa ttttcactat taccagaaaa tgaaactgga atgactctgt ttgccaataa
      421 caccgttaca ggagaatata ataacggcgg ggccatattt gctaaagaaa actcaacgct
      481 gaatcttact gatgttattt tttccggtaa cgtcgcaggc ggctatggtg gcgcaatcta
      541 ttcttctggt actaacgata ctggtgccgt cgatttacgt gtcactaacg ccatgtttcg
      601 caataacatc gctaatgatg gcaaaggtgg cgcaatttat accattaata atgacgttta
      661 tttaagtgat gttatttttg ataacaacca ggcatataca tcaacaagtt acagtgatgg
      721 cgatggcggg gcaatcgatg ttaccgataa taatagcgac agcaagcatc cttcaggtta
      781 tacgatagta aataacactg cctttacaaa taacactgcc gaaggttatg gcggggcgat
      841 atataccaat agcgtgacgg ctccctatct tattgatatt tctgttgatg acagctacag
      901 ccagaacgga ggcgtgttag tcgatgagaa caatagcgca gcaggctatg gagatggtcc
      961 ttcctctgcg gcgggtggct ttatgtatct cggcttaagt gaagttacct ttgatattgc
     1021 cgacggaaaa acgctggtta ttggcaatac agagaatgac ggagctgttg actctattgc
     1081 tggtaccggg ttaatcacca aaacaggttc cggcgatctg gtacttaatg cagataacaa
     1141 tgactttact ggtgagatgc agattgaaaa cggtgaagtt accctgggcc gcagcaactc
     1201 cctgatgaat gtcggcgata cgcattgcca ggacgatccg caagactgct acggtctgac
     1261 gatagggagt attgatcagt atcagaatca ggctgagcta aacgttggct cgacccaaca
     1321 aacttttgtg cacgcattga cgggctttca gaatggcact ttaaatatcg atgctggtgg
     1381 caacgttact gttaatcagg gcagttttgc tggcatcatc gaaggtgctg gtcagctcac
     1441 cattgcgcaa aacggcagct acgtgctggc aggggcgcag tcgatggcgc taaccggcga
     1501 tatagtcgtt gatgatggtg cggtgctttc gctggaaggc gacgcggcag atcttaccgc
     1561 tctccaggac gatccgcagt cgatcgtgtt aaacggcggt gtgctcgatc tctctgattt
     1621 ctccacctgg cagagcggca catcatacaa cgatggcctt gaagtcagtg gcagcagcgg
     1681 aacggttatc ggcagtcagg atgtggtaga tcttgcaggt ggcgacaatt tgcatatcgg
     1741 cggcgacggg aaagatggcg tctacgtggt ggtcgatgcg agcgacgggc aggtaagtct
     1801 ggcaaacaat aatagttatt tgggcacaac acaaatcgcc tccggtacgc tgatggtgag
     1861 cgacaactcg cagcttggag atacccacta taaccgccag gttatcttta ccgataagca
     1921 acaagaaagc gtgatggaga ttacctccga cgttgacacg cgttcagatg cggcaggcca
     1981 cggacgtgat attgaaatgc gcgccgacgg tgaagtggca gttgatgcgg gggtagacac
     2041 gcagtggggc gcactgatgg ctgacagcag cgggcagcat caggatgagg gtagcacatt
     2101 gactaaaacg ggggctggta cactggagct gaccgccagc ggtacaacgc agtcggcggt
     2161 acgtgtcgaa gaaggcaccc tgaaaggtga tgttgctgat atccttcatt atgcttcgtc
     2221 actgtgggtt ggtgatgggg caacgttcgt tactggcgcg gatcaggata ttcagtcaat
     2281 tgatgctatt tccagcggca ctatcgacat cagcgatggt acggttttgc gcctgaccgg
     2341 gcaggatact tccgtcgccc ttaatgcctc actatttaac ggcgatggga cgctggtgaa
     2401 tgccaccgat ggtgtgacgt tgacaggtga gcttaatacc aaccttgaaa ctgacagcct
     2461 gacttatctt tccaacgtga cggttaatgg caatctgacc aatacgtccg gtgcggttag
     2521 cctgcaaaat ggcgtcgctg gcgatacgct gacggtaaac ggtgattata ccggcggcgg
     2581 tacgctactg ctcgatagcg aattaaacgg cgatgactcg gtaagcgatc aattggtgat
     2641 gaacggtaat actgctggca acacaactgt ggtggttaac tccattacag ggattggtga
     2701 gccgacatcg acaggcatta aagtggttga tttcgcagct gatcccacgc agtttcaaaa
     2761 caatgcgcag ttcagtctgg caggcagcgg ctacgtcaat atgggagcgt atgactacac
     2821 gctggtggaa gataacaacg actggtatct gcgatcgcaa gaagtaacgc cgccatcgcc
     2881 acctgatcca gacccgactc ccgatcctga tcccacgccg gatcctgatc caacacccga
     2941 cccggaacct acgcctgctt accagccggt gttgaatgcc aaagttggcg gttatctcaa
     3001 taacctgcgg gcggcaaatc aggcgtttat gatggagcga cgcgatcacg caggtggcga
     3061 tggtcagacg ctgaatttac gtgttatcgg cggagattat cattacacag cagcggggca
     3121 actggctcaa catgaagaca cttctacggt gcaacttagc ggcgatctgt ttagcgggcg
     3181 ctggggcacg gatggcgagt ggatgcttgg gattgttggt ggctacagcg ataaccaggg
     3241 cgacagccgc tcgaatatga ccggaactcg cgccgataac cagaaccacg gttatgccgt
     3301 tgggctgaca tcaagctggt ttcagcacgg taatcagaag caaggggcct ggctggatag
     3361 ctggctgcaa tacgcgtggt ttagcaatga tgtttccgaa caagaagatg gcacagatca
     3421 ttaccactcg tcggggatta tcgcctcgct ggaggcgggg tatcagtggt taccggggcg
     3481 tggtgtggtg attgaaccgc aggcgcaggt gatttatcag ggcgtgcagc aggatgattt
     3541 taccgccgct aaccgtgcgc gcgtgtcaca atcgcagggt gatgatattc agacgcggct
     3601 gggtttacac agcgaatggc gtaccgctgt tcatgtcata ccaacattag atctgaatta
     3661 ttatcacgat ccccattcga cggaaattga agaggatggc agcactatca gtgacgatgc
     3721 ggtgaagcaa cggggtgaaa taaaagtggg agtcacgggc aatatcagtc agcgagtgtc
     3781 cctgcgcggc agcgtggcgt ggcagaaagg gagtgatgat tttgcccaga cggcagggtt
     3841 tttgtcgatg acggtgaaat ggtaatcact gtctagtcgg cacggttgcc ggatgcggcg
     3901 taaacgcctt atccggccta cggggagcat ttgtaggcct gataagacgc ggcaagcgtc
     3961 gcatcaggca ttggcgcggc caaacatcaa cctcacttat tctgcgtgtg cagcatatag
     4021 ttcacatcca cgccggggcc gagtttaaaa gtattagtga tcgggttgta atgcagccca
     4081 gtgatatgcc gctcttttaa actggtctga tccacccagc ccagcaattc tgccggttta
     4141 ataaacttct tcacatcatg cgtgcctttg ggcaccatgc gcaaaatata ttccgcacca
     4201 accaccgcca tcagccatga cttgccgttg cggttaagtg tcgagaaaaa gacatcgccg
     4261 cctggtttca ccagttgcgc acaggctctg accactgact gcggatcggg gacgtgctcc
     4321 agcatctcca tgcaggtcac cacatcatac tgcccggcat gttttgccgc gtgctcttcc
     4381 acggtttcct gcacataatc cacctgaatg ccgctttcca gcgcgtgcag ttttgccacc
     4441 tgcaatggct caaagcccat atccagaccg gtcaccgtcg cgccttcgcg cgccatactc
     4501 tcggccagaa tgccgccgcc acaaccgaca tcgagcacct ttttgccaaa taaaccgcca
     4561 gcacgctcgg caatatagcc cagacgcagc gggttaatgc ggtgcaacgg tttgaactca
     4621 ccttcaagat cccaccagcg ggaggcgacg gcttcaaatt tagcgatctc ttcgtggtct
     4681 acgttatggt tttccggcga tttttcggca ttcattggca cttctactcc gtaattggca
     4741 agacaaacga gtatatcagg cattggatgt gaataaagcg tataggttta cctcaaactg
     4801 cgcggctgtg ttataatttg cgacctttga atccgggata cagtagaggg atagcggtta
     4861 gatgagcgac cttgcgagag aaattacacc ggtcaacatt gaggaagagc tgaagagctc
     4921 ctatctggat tatgcgatgt cggtcattgt tggccgtgcg ctgccagatg tccgagatgg
     4981 cctgaagccg gtacaccgtc gcgtacttta cgccatgaac gtactaggca atgactggaa
     5041 caaagcctat aaaaaatctg cccgtgtcgt tggtgacgta atcggtaaat accatcccca
     5101 tggtgactcg gcggtttatg acacgatcgt ccgtatggcg cagccattct cgctgcgtta
     5161 catgctggta gacggtcagg gtaacttcgg ttccatcgac ggcgactctg cggcggcaat
     5221 gcgttatacg gaaatccgtc tggcgaaaat tgcccatgaa ctgatggccg atctcgaaaa
     5281 agagacggtc gatttcgttg ataactatga cggcacggaa aaaattcccg acgtcatgcc
     5341 aaccaaaatt cctaacctgc tggtgaacgg ttcttccggt atcgccgtag gtatggcaac
     5401 caacatcccg ccgcacaacc tgacggaagt catcaacggt tgtctggcgt atatcgatga
     5461 tgaagacatc agcattgaag ggctgatgga acacatcccg gggccggact tcccgacggc
     5521 ggcaatcatt aacggtcgtc gcggtattga agaagcttac cgtaccggtc gcggcaaggt
     5581 gtatatccgc gctcgcgcag aagtggaagt tgacgccaaa accggtcgtg aaaccattat
     5641 cgtccacgaa attccgtatc aggtaaacaa agcgcgcctg atcgagaaga ttgcggaact
     5701 ggtaaaagaa aaacgcgtgg aaggcatcag cgcgctgcgt gacgagtctg acaaagacgg
     5761 tatgcgcatc gtgattgaag tgaaacgcga tgcggtcggt gaagttgtgc tcaacaacct
     5821 ctactcccag acccagttgc aggtttcttt cggtatcaac atggtggcat tgcaccatgg
     5881 tcagccgaag atcatgaacc tgaaagacat catcgcggcg tttgttcgtc accgccgtga
     5941 agtggtgacc cgtcgtacta ttttcgaact gcgtaaagct cgcgatcgtg ctcatatcct
     6001 tgaagcatta gccgtggcgc tggcgaacat cgacccgatc atcgaactga tccgtcatgc
     6061 gccgacgcct gcagaagcga aaactgcgct ggttgctaat ccgtggcagc tgggcaacgt
     6121 tgccgcgatg ctcgaacgtg ctggcgacga tgctgcgcgt ccggaatggc tggagccaga
     6181 gttcggcgtg cgtgatggtc tgtactacct gaccgaacag caagctcagg cgattctgga
     6241 tctgcgtttg cagaaactga ccggccttga gcacgaaaaa ctgctcgacg aatacaaaga
     6301 gctgctggat cagatcgcgg aactgttgcg tattcttggt agcgccgatc gtttgatgga
     6361 agtgatccgt gaagagctgg agctggttcg tgaacagttc ggtgacaaac gtcgtactga
     6421 aatcaccgcc aacagcgcag acatcaacct ggaagatctg atcactcagg aagatgtggt
     6481 agtgacgctc tctcaccagg gctacgttaa gtatcagccg ctttctgaat acgaagcgca
     6541 gcgtcgtggc gggaaaggta aatctgccgc acgtattaaa gaagaagact ttatcgaccg
     6601 actgctggtg gcgaacactc acgaccatat tctgtgcttc tccagccgtg gtcgcgtcta
     6661 ttcgatgaaa gtttatcagt tgccggaagc cactcgtggc gcgcgcggtc gtccgatcgt
     6721 caacctgctg ccgctggagc aggacgaacg tatcactgcg atcctgccag tgaccgagtt
     6781 tgaagaaggc gtgaaagtct tcatggcgac cgctaacggt accgtgaaga aaactgtcct
     6841 caccgagttc aaccgtctgc gtaccgccgg taaagtggcg atcaaactgg ttgacggcga
     6901 tgagctgatc ggcgttgacc tgaccagcgg cgaagacgaa gtaatgctgt tctccgctga
     6961 aggtaaagtg gtgcgcttta aagagtcttc tgtccgtgcg atgggctgca acaccaccgg
     7021 tgttcgcggt attcgcttag gtgaaggcga taaagtcgtc tctctgatcg tgcctcgtgg
     7081 cgatggcgca atcctcaccg caacgcaaaa cggttacggt aaacgtaccg cagtggcgga
     7141 atacccaacc aagtcgcgtg cgacgaaagg ggttatctcc atcaaggtta ccgaacgtaa
     7201 cggtttagtt gttggcgcgg tacaggtaga tgactgcgac cagatcatga tgatcaccga
     7261 tgccggtacg ctggtacgta ctcgcgtttc ggaaatcagc atcgtgggcc gtaacaccca
     7321 gggcgtgatc ctcatccgta ctgcggaaga tgaaaacgta gtgggtctgc aacgtgttgc
     7381 tgaaccggtt gacgaggaag atctggatac catcgacggc agtgccgcgg aaggggacga
     7441 tgaaatcgct ccggaagtgg acgttgacga cgagccagaa gaagaataat tttacttctt
     7501 catgccaaaa gggagctatc tcccttgttt gaattgaaaa gtccaggctg caaagtctgg
     7561 gcttttgtcg tattagggca cggtaaagtt tggctgtgcc cgtaaaaaat ggctggctat
     7621 acacaaggaa tgtggcaatg agtggtgaaa aaaaggcgaa aggctggcgg ttctatggtc
     7681 ttgtaggttt tggcgcaata gcactgcttt ccgctggcgt ctgggcgttg caatatgctg
     7741 gcagtgggcc agaaaaaacg ttgtcgccgc tggtggtgca caacaatctg caaatcgatc
     7801 tcaatgagcc ggacctcttt ctcgacagcg actctctgag ccagcttccc aaagatctcc
     7861 tcaccattcc gtttctccac gatgttctga gcgaagattt cgttttctat tatcagaatc
     7921 atgccgatcg tctgggcatt gaaggcagca ttcgtcgtat tgtctatgaa cacgatctca
     7981 cgctgaaaga taagctcttt tcgtcactct tagatcagcc cgcgcaggca gcgctgtggc
     8041 acgataaaca aggccatctt tcacattata tggtgctgat ccagcgcagt ggtttaagca
     8101 aactgctgga gccattgttg tttgccgcta ccagcgacag ccagttaagc aaaacggaaa
     8161 tcagtagcat caagataaat agtgaaacta ttcctgttta tcagttgcgc tataacggca
     8221 ataacgccct gatgttcgcg acatatcagg acaagatgct ggtgttttcc agcacggata
     8281 tgctgtttaa agatgatcag caggataccg aagccacggc gatcgcaagt gatttgttga
     8341 gcggcaaaaa acgctggcaa gccagctttg gcctggaaga gcgtgctgcc gaaaaaacgc
     8401 cagtacgcca gcgcatcgtg gccagcgcca ggttgctggg ttttggctac cagcgattaa
     8461 tgccttcttt tgctggcgta cgcttcgaaa tgggtaacga cggctggcac agttttgtgg
     8521 cgttaaatga tgaatccgcc agcgtagatg ccagtttcga ttttacgccg gtctggaaca
     8581 gtatgcctgc cggggccagc ttctgtgtgg cggtgccgta ttcacacggt attgccgaag
     8641 agatgctttc gcacatcagc caggaaaacg acaagctgaa tggggcgtta gacggtgccg
     8701 cggggctgtg ctggtatgaa gactcaaaat tgcaaacccc gctgtttgtc ggtcagtttg
     8761 atggcactgc cgaacaggcg caattgccag ggaaaatgtt tacgcaaaat attggtgcgc
     8821 acgaaagcaa agcgccagaa ggtgttttgc cggtaagcca gactcagcag ggcgaagcgc
     8881 aaatctggcg tcgcgaagtg agttcccgat acggccagta tccgaaagcg caggcggcgc
     8941 aacccgatca attaatgtcg gattattttt tccgtgtgtc gctggcgatg caaaacaaaa
     9001 cgctgctttt ctccctcgat gacacgctgg ttaataacgc gctgcaaaca ctgaataaaa
     9061 cccgcccggc aatggtggat gtaataccca ctgatggcat cgttccgctc tatatcaatc
     9121 cacaaggaat agcgaaactg ctgcgtaacg aaacgttgac cagtctgccg aagaatctcg
     9181 aaccggtttt ttataacgcc gcacaaactt tattaatgcc gaagctggac gctttatctc
     9241 aacaaccgcg ttatgtcatg aagctggccc agatggaacc cggtgccgcc tggcagtggc
     9301 tacccataac ctggcagccg ctatgaggca cgggctgctg gcgctgattt gctggctgtg
     9361 ttgtgttgtt gcccatagcg aaatgctgaa tgtcgaacaa tccgggctgt ttcgcgcctg
     9421 gtttgtacgc attgcacaag aacagctccg ccaggggcca agtccacgct ggtatcagca
     9481 ggattgtgcg ggcctggtgc gatttgcagc gaacgaggcg ctgaaagttc acgacagcaa
     9541 atggctaaaa agtaacggca tagcaagtca gtatttgccg cctgaaatga cgttaaaacc
     9601 tgaacagcgt caactggcgc aaaactggaa tcaggggaac gggaaaaccg gtccctatgt
     9661 gaccgcgatt aatttgattc agtacaacag ccagtttatt ggccaggaca taaaccaggc
     9721 gctgcctggc gatatgattt ttttcgatca gggcgatgcc cagcacttaa tggtctggat
     9781 ggggcgttac gtcatctacc acaccggaag cgccacgaaa actgacaacg gaatgcgcgc
     9841 agtcagtctg caacaactta tgacatggaa ggacacccga tggatactca acgattccaa
     9901 tcccaatttc attggcattt atcgtttaaa ttttctggcg cgatagcagc gtgcttgtcc
     9961 ctgtctcttg tgggaacagg gcttgctaat gctgatgatt cgcttccttc cagtaactat
    10021 gcgccgcccg cagggggaac attctttttg cttgctgaca gcagttttag cagcagtgaa
    10081 gaggcgaaag tgcgactgga agcgccgggg cgtgattatc ggcgctatca gatggaagag
    10141 tacggcggcg tggacgttcg cctgtatcgt attcctgacc cgatggcatt tttgcgccag
    10201 cagaaaaacc tgcatcgcat tgtggtgcaa ccgcaatatt tgggcgacgg gctgaacaat
    10261 acgctaacct ggctgtggga taactggtac ggcaaatctc gccgcgtgat gcagcgtact
    10321 ttctcttctc agtcaaggca gaatgtgact caggcgttac ccgaattaca gctcggcaat
    10381 gccattatta aaccttcccg ttatgtacag aacaaccagt tttccccgct gaaaaaatat
    10441 cccctggtgg aacagttccg ttatccacta tggcaggcta aaccgttcga gccgcagcaa
    10501 ggggtaaaac tggaaggcgc atccagcaat ttcatctcgc cgcagccggg taacatttat
    10561 attcctctcg gccaacaaga gccgggactg tacctcgtcg aggcgatggt tggtgggtat
    10621 cgggcgacga cggtggtgtt tgtttccgat accgtggcgc ttagcaaagt gtcaggcaaa
    10681 gagcttctgg tgtggaccgc gggtaaaaaa cagggtgaag cgaagcccgg ctcagagatc
    10741 ttgtggactg acggtcttgg cgtgatgacc cgcggtgtga ccgatgacag cggtaccttg
    10801 cagttacaac atatatcgcc agaacgttca tacattctgg gtaaggatgc tgaaggcggc
    10861 gtttttgtct ccgagaactt cttctacgaa agcgaaatct acaacacccg cttgtatatt
    10921 tttaccgatc gcccgctata tcgcgcaggc gatcgtgtcg atgttaaagt gatcggccgc
    10981 gagttccacg atccgttgca ttcatccccc atcgtcagcg ccccggcgaa gctttcggtg
    11041 ctggacgcca acggcagtct gttgcaaacc gtcaatgtca cgctggatgc gcgcaatggc
    11101 gggcagggaa gtttccgcct gccagaaaat gccgtagccg gaggttatga gttacgtctt
    11161 gcttaccgca atcaggtcta tagcagcagt tttcgcgtgg caaactacat caagccacat
    11221 ttcgagattg gtttagctct cgacaaaaaa gagttcaaaa ctggcgaagc ggtcagcggc
    11281 aaactgcaac tgctctaccc ggatggcgag ccggtaaaaa atgcccgcgt gcagttaagt
    11341 ttgcgcgctc agcaattatc aatggtcggt aacgatttgc gttatgccgg acgtttcccc
    11401 gtgtcgctgg aaggcagcga aacggtgtcc gacgccagtg gtcatgtgac gttaaatctc
    11461 cccgccgccg ataaaccgag ccgctatttg ttaaccgtct ccgccagtga cggcgcggcg
    11521 tatcgcgtca ccaccaccaa agagatcctc attgaacgcg gcctggcgca ttactcttta
    11581 agtaccgccg cacaatacag taatagcggc gagtcggttg tgttccgtta tgccgcgctg
    11641 gaatcttcaa aacaggttcc tgttacgtat gaatggttgc gtctcgaaga ccgcacgagc
    11701 catagcggag agctaccgtc aggcggcaaa tcctttaccg tcaatttcgc taaacctggc
    11761 aactacaatc tgacattacg cgataaagac ggcttaattc tcgctgggtt aagtcatgcc
    11821 gtcagcggta agggcagcac ggcgcatact ggtacggtag atatcgtggc ggataaaacg
    11881 ctgtaccagc caggcgaaac cgcgaagatg ctgattacct ttccggagcc aattgatgaa
    11941 gcattattga cgctggaacg cgatcgcgtg gaacagcagt cgctgctttc gcatccggca
    12001 aactggctaa cgctacaacg tttaaacgat acccagtatg aagcccgggt tcgagtgagc
    12061 aattcctttg cgcctaacat cactttttcg gtgctgtata cccgtaatgg tcagtacagt
    12121 tttcagaacg ccgggatcaa agttgccgtt cctcagcttg atatccgggt gaaaacggac
    12181 aaaacccatt accagcctgg tgaactggtc aatgtcgaat taacctcgtc gctgaaaggt
    12241 aaacctgttt ctgcgcagct aacggtaggc gtggtcgatg aaatgatcta cgcgctgcaa
    12301 ccagaaatcg cgccgaatat cggcaaattt ttctatccgc tggggcgtaa caatgtgcgt
    12361 accagctcca gtctgtcgtt tatcagctac gaccaggcgc tctccagcga gccggttgcg
    12421 cctggcgcga ctaaccgcag cgagcggcga gtaaaaatgc ttgaacgtcc acggcgtgaa
    12481 gaggtggata ccgcggcatg gatgccgtca ctcacaaccg ataaacaagg caaagcgtat
    12541 ttcacgttcc tgatgcctga ttcgttaacc cgctggcgta tcaccgcgcg tgggatgaac
    12601 ggcgacgggc tggtcgggca ggggcgtgct tatctgcgtt cggaaaaaaa tctctacatg
    12661 aagtggagta tgccaacggt gtatcgcgtg ggcgacaaac cggcggcagg actgtttatc
    12721 ttcagtcagc aggataacga accggtggcg ctggtgacta aatttgcagg cgctgagatg
    12781 cgccagacgc tgacgctgca caaaggggcg aattatattt cgctgacgca gaatattcag
    12841 caatctggct tgttaagtgc agaactgcaa caaaatgggc aagtgcagga cagcattagc
    12901 acaaaactgt cttttgtgga taacagctgg cccgttgaac agcagaaaaa tgtcatgctc
    12961 ggtggtggcg ataacgcgct gatgttgccc gagcaggcga gcaatatccg gctacaaagt
    13021 agtgaaacgc cgcaggagat tttccgcaac aatcttgatg cgttagtcga tgaaccgtgg
    13081 ggtggcgtaa tcaacaccgg tagccgtctg atcccgctca gtctcgcctg gcgttcgctt
    13141 gccgatcatc aaagtgccgc cgctaacgac attcgtcaga tgattcagga taaccgtctg
    13201 cggctgatgc aactggcggg gcccggagcg cgctttacct ggtggggtga agatggcaat
    13261 ggtgacgcct tccttacggc atgggcatgg tacgccgact ggcaggccag ccaggcgatc
    13321 ggcgtaacgc aacaaccgga atactggcag catatgctcg acagttatgc cgagcaggca
    13381 gataacgtgc cgttattgca tcgggcgctg gtgctggcgt gggcacagga gatgaatctg
    13441 ccgtgcaaaa cgttgttgaa agggttggat gaagctatcg cccggcgcgg aactaaaact
    13501 gaagatttct ctgaggaaga ccccagcgat atcaatgaca gcctgatctt cgatacaccg
    13561 gaatctccac tggcagatgc ggtggcaaac gtcttaacca tgacgttgct gaaaaaagcg
    13621 cagttgaagt ccacggtgat gccacaggtt cagcaatatg cgagggataa agcggcaaac
    13681 agcaatcagc cgctggcgca cacggttgtg ctgcttaata gcggtggcga cgctacccag
    13741 acggccgcta ttttaagtgg tttgaccgct gagcaatcca ctattgaacg cgcgctggcc
    13801 atgaactggc tggcgaaata tatggcgaca atgcctccgg ttgtgttgcc tgcgcctgcg
    13861 ggcgcatggg ccaaacataa gttaactgga gggggcgaat actggcgttg ggttggtcag
    13921 ggtgtgccgg acattctctc ttttggtgac gaattatcgc cgcaaaatgt gcaggtccgc
    13981 tggcgtgaac cggcaaaaac ggctcaacaa agtaacattc cggtaaccgt tgaacgccag
    14041 ttgtatcggc ttatccctgg tgaagaagag atgagcttta ctctgcaacc ggtgaccagc
    14101 aatgagattg acagcgatgc gctgtatctc gatgaaatca cgcttaccag cgagcaggat
    14161 gcagttctgc gctacggtca ggtggaagta ccgctcccac cgggagccga cgttgagcgc
    14221 acaacatggg gcatttcggt caataaaccc aacgccgcga aacaacaggg gcaattgctg
    14281 gaaaaagcac gtaatgaaat gggcgaactg gcctatatgg tgccggtgaa agaactgacg
    14341 ggaacggtca ctttccgcca tttgctgcgc ttctcgcaaa aagggcaatt cgttctgcct
    14401 cctgctcgtt atgtgcgttc ctatgcacct gcacagcaaa gtgttgcggc agggagtgaa
    14461 tggaccggga tgcaggtgaa ataagtgaac tggcgcagaa tcgtctggct attggcgttg
    14521 gttactctgc caacgcttgc ggaggagacg ccgttacaac tggcgctgcg gggcgcgcag
    14581 cacgatcaac tgtatcagtt gtcgtcatca ggcgtgacca aagtctcggc attacctgac
    14641 acgctcacca cgccgttagg gagtttgtgg aaactgtatg tttacgcctg gctggaggat
    14701 acccatcaac cggagcagcc ctaccagtgt cgcggaaact ccccggaaga ggtttattgt
    14761 tgtcaggctg gggagagcat aacgcgcgat accgcgctgg tacgttcctg tgggctctat
    14821 tttgcgccgc agcggttgca tattggcgca gatgtgtggg ggcaatactg gcaaaagcgt
    14881 caggcacctg cgtggttagc gtctcttaca acgctgaagc cagaaacctc ggtaacggtt
    14941 aaatcgctgc tcgattcatt agcgacgtta cctgcgcaaa acaaagctca ggaagtctta
    15001 ctggatgtgg tgctggacga ggcgaaaatc ggtgtcgcat ccatgctcgg tagtcgggta
    15061 cgggtgaaaa cctggagctg gtttgccgat gataaacagg aaatccgcca gggtggtttt
    15121 gctggctggt taaccgatgg tacaccgctg tgggttactg gcagtggcac cagcaaaacg
    15181 gtactaaccc gttatgcgac ggtgcttaat cgggttttgc cagtgccgac gcaggttgcc
    15241 agtgggcaat gtgtagaggt ggagctgttt gcccgctatc cgctgaagaa aatcacagcg
    15301 gaaaagagta cgacggcagt aaaacccggc gtacttaatg ggcgctatcg tgtgacgttt
    15361 actaatggta accacattac gtttgtcagc cacggtgaga cgaccttact gagcgaaaag
    15421 ggcaaactga aattgcagtc acatctcgat cgtgaagagt acgttgccag agttctggat
    15481 cgcgaggcga aaagtacgcc ccctgaggca gcgaaagcca tgaccgtggc gattcgcact
    15541 tttttacagc aaaacgccaa tcgggaaggc gattgcctga ccatccctga cagcagcgcc
    15601 acgcagcgtg tttcggcttc gccagctacg acgggggcgc gaacgatggc cgcctggacg
    15661 caggatctca tctatgcggg cgatccggtt cattatcatg gcagtcgcgc taccgaaggg
    15721 acgctttcct ggcggcaagc cacggcccag gcggggcagg gagagcgtta cgaccagata
    15781 ctcgcgtttg cttatcccga caacagcctt agccgctggg gagcgccacg ctcaacctgc
    15841 cagttattac ccaaagcgaa agcctggctg gcgaaaaaaa agccgcagtg gcggcgtata
    15901 ttacaagctg agacggggta caacgaacca gacgtgtttg cggtttgccg tttagtctca
    15961 ggtttcccct ataccgatcg gcagcagaaa cggctgttta ttcgtaactt ctttacgctt
    16021 caggatcggc tggatttaac ccatgagtat ctgcacctgg cctttgatgg ttatcccacc
    16081 gggcttgatg agaactatat cgaaacgctg acccgacaat tattaatgga ctaatgctat
    16141 gcgaaagatt tttcttccgt tgttactggt ggcactttcg cctgtcgttc acagtgaagg
    16201 tgtgcaggaa gtcgagattg acgcaccgct ctctggctgg catccggtag agggcgaaga
    16261 tgccagtttt tcacaatcga tcaattaccc ggcgtcgtcc gtcaacatgg cagatgatca
    16321 aaatatctca gcgcagatcc gcggcaaaat aaagaattat gctgcggcgg gtaaagttca
    16381 gcagggccgg ctggtggtca acggtgccag tatgccgcag agaattgaat ccgatggttc
    16441 atttgcacgc ccttatattt tcactgaagg cagcaacagc gtgcaggtca tcagcccgga
    16501 tgggcaaagc cgacaaaaaa tgcagtttta ctcaacgccg ggtacaggaa cgattcgtgc
    16561 acgtttacgg ctggttctct cgtgggatac ggacaatacc gatctcgatc tccatgttgt
    16621 cacgcccgat ggcgaacacg cctggtacgg taacacggtg ctgaaaaaca gtggtgcact
    16681 ggatatggat gtcacgacgg ggtacggacc cgagattttc gccatgccag cgccagttca
    16741 cggacgctat caggtgtata tcaactacta tggcggacgc agcgaaacgg aattaaccac
    16801 cgcccaactg acgctgatca ccgatgaagg atcggtcaat gagaaacagg aaacatttat
    16861 tgtaccgatg cgtaatgctg gcgagctgac gctggtgaaa agttttgact ggtgattaat
    16921 ggggttacct tcgctgtcga tgttcaagaa aacgcccgat aactttcgct atcgggcgtt
    16981 tttgttcgtt aattcaaccg ttcaatcacc atcgcaattc cctggccgcc accaatgcac
    17041 agtgttgcca gccccagcgt tttatcgcgt gcctgcatgg catgtaatag tgtgaccaga
    17101 atacgagcac cactggcacc gataggatgc ccgagcgcga tggccccgcc gttgacattc
    17161 actttctcag gatcaaagcc caaggttttc ccaacggcaa ggaactgtgc agcaaatgct
    17221 tcattagctt cgatgagatc aatgtctgcc agttgcagcc ccgccagttg taacgctttt
    17281 tgcgtggcag gtactggccc catacccatc aatgcgggag gcacgccacc gctggcataa
    17341 cttttaatgc gagccagggg ggtaaggcct gctgccagcg ccgcagattc ttccataatc
    17401 accagagcgg cagcaccgtc gttaatacca gacgcgttcc cagcggtgac tgttcctgct
    17461 ttatcgaagg ccgggcgcaa tgcacctaac gcttcagccg ttgaattcgc tttcgggaat
    17521 tcgtcttgac tgaagacgaa ggttttcttc cgagtgacaa catttaccgg gacgatttcg
    17581 gctgtaaaag caccggactc aattgcggct gccgctttac gctgtgaatg tagcgccagt
    17641 tcatcctgca tttcacgggt aattccgtac tctttagcca cgttttcggc ggtaatcccc
    17701 atatgataac catgggtggc gcacatcagg ccatcgcgca ggattacgtc ataaacctgt
    17761 ccgtctccaa gacgataacc agagcgtgct tttgcatcga gtaagtaggg cgctaaactc
    17821 atattttcca taccccccgc cacaatgctc tgcgcctgac ctgcctgaat tgcctgggcg
    17881 gcaagcgcca cacttttaag acccgaacca cacactttat tgaccgtgaa tccgcacacc
    17941 gtttctgcca gcccgctttt taacagtgcc tgacgtgccg gattttgccc caacccggct
    18001 tgcaacacgt tacccataat cacttcatca atgtgtagtg aatcgatttt tgcacgttca
    18061 atggcggctt taattactgt cgcccccagg tcgatggcgc tggtggaagc gagtgaaccg
    18121 ttaaaactac cgatagcagt acgtaccgca ctgacgatga cacaattttt cattttatat
    18181 tccttctgtc tgtagggttg ccgtcagaac agcgttaaac caatgacgaa aatgacgccg
    18241 gagaagagca gggcagtgat gcaatagccc atgatgtcgc ggacaccgag tccggcgatt
    18301 gccagtgctg gcagcgccca gaatggttgt gccatgttca tccattgctc gccgtaggcg
    18361 atcgccatta ccgatttacc gagatcagcg cccagcgcct gggctgcggg tatcacgaaa
    18421 ggtccctgaa taacccagtg accgccgcca gacggaacgg cgaagttaat cagtgcagaa
    18481 ctaaaaaagg tcattaccgg gaaggtgtct ttgttcgcaa cattgatgaa gaattcggta
    18541 atgagtccac ccagaccaga atgctccatc atcagttgga tcccagcgta gaaggggaat
    18601 tgcaccagaa taccggcagt actgcgtgct gccgcgctga tagcacgcat ataagccatt
    18661 ggcgttttat gtagcaacag acccgcaatc ataaacatca ggttgacggt attgatggtg
    18721 atgttgaagc catgttcgct gaagtacatc gccaggtaag cgataccgag tgcgccgatg
    18781 atcaacgtca gaatgcggct ttcttccagt cgctcggatg gtggggcatc tttcggtagc
    18841 tgcttttgaa aatcagcctc ttccatgagt agtttcggat cgatactcac cacgtcagac
    18901 ggttttggca tcatcatgcg ggtgataaat ggcatcacca caatcaacgt cacagtgatg
    18961 aaaatgttaa aaccactgaa cagagtatcg cccaccggga tcagcccggc gatatgctca
    19021 accgggttgc ccggtgttgc agccaacaga ggcattgatc cagagaagcc gccaccccag
    19081 gtgagaaaac caatgtaggc gcaggcaatg agcaacggat aatcagaacc ggggactcgc
    19141 cgggcgactt cacgggcaaa cattgcgccg acaaccaaac caaatcccca gttgatgaca
    19201 caagcgactg aaccgaagaa agtgaccagc atgacgccct gtacgggcgt ctttgcggcg
    19261 gaggcggcag tacgcagcaa acttttcacc ggagcagagc tggcaagggc atgaccggta
    19321 acgatgataa gcgccatctg cataccaaac gccagcaagt tccagaaacc gtcaccccac
    19381 attttcacca tgctgatcgg cgtttgtggt gttaaccaaa gcgcgatcac gaatgttagc
    19441 aatgtcagca acatggcaaa gatcagtgga tcgggaagcc accggctgac aaaacgcgtc
    19501 ataaaacgcg atatgcgacc aatcataaat caccccgttg cgtattcaga tcggcggcga
    19561 cttcaaaccg agcttctgtt ttggcacgca cggtggctaa atcacacccg tcggcaattt
    19621 cggtgagcca cattttgccg tcaataaaac gaaagacagc cagttcagta accagcatat
    19681 gcaccgcatg ttgcgcagtg agtggcatgg tgcagcggcg caaaattttt gctgaaccat
    19741 ctttggcgca atgttccatg gcgatgatca ctttgcgcga cccggtcacc agatccatcg
    19801 cgccacccat accgggcacc attttcccag gcactaccca gttcgcgagg tttgcttctt
    19861 cgtctacttg caaaccgccg agcacgcagg catcaatatg accgccacgg attagcgcaa
    19921 atgacatggc gctatcaaac atggctgcac cgggtaaaac accgcacggt tgcccgccag
    19981 cgttcaccag atctggatgc gctgtcgtga ccgggcctaa accgaggaag ccgttttccg
    20041 attgcagagt gatatgaata ccctccggta aataattggc gaccattgtg ggtaaaccga
    20101 tccctaagtt aacgatgtca ccatcacgaa gctcttgcgc cacacggcgc gcaatacgtt
    20161 gtttcgcatc cattatttgc tctcctgtga aacgatgatg tggtcgataa cggcaccagg
    20221 ggtgacaata tggtcaggtt gcagctcgcc ggtttcgacc agttcatctg gctctaccag
    20281 cgtgatatca gccgcaaggg ctatcagggg gttaaagttg cgggcgctaa gttgataggt
    20341 caggttgcca agtgtgtcgc aacgatgagc gcgaattagc gccaggtcgg cgcgcagtgg
    20401 gcgttcgagc agccaagttt taccgtcgag tgtcagtgtc tgtttgcctt cctctacgac
    20461 ggtgccgaca cccgttgggg tgagaaaacc accaagtcca gctccaccac agcgaatttg
    20521 ctcgattagc gtaccttgcg gcaccagaac gacgtccatc tcaccagata tcatgcgccg
    20581 acctgtttcc gggttggtgc cgatatgtga agcaatcact ttgcggactc gaccattgac
    20641 gatgagcgga ccgatgccgg tatcaacaaa cgcggtatca ttggctatca atgtcaggtc
    20701 gcgaacacca gattccagta atgcttcaac caggcgggat ggagtgccaa tccccataaa
    20761 tccgcccacc atgatggtca tgccgtcacg aaagaagccg gtggcgtctt gtaatgtcat
    20821 caattttgtt ttcatttttt atccctcttg catacaccgt tgtgggtaca gtcaggcaat
    20881 agcaagggga gtgccagaat gagattattg gatttagtta tttaactaat tgaatatgaa
    20941 gggataaatt ggatggggat ttataaggag aatgagcggc tatgcagaaa attgcgcact
    21001 gtgcaaattt ctgcatagca agttttggtg ttatacatcc gccggatcga taccgtattc
    21061 ctggagttta tacatcaatg cacggcgact gatgcccagc attaaagcag tgcgggttcg
    21121 gtttccttct tgttgttcca gcacttccat aatgatgcgt ttttcgacgc gtttaatttc
    21181 ctcttttaaa ttacgctcac cgacaggggc tgtttttacc tcgccagcat tacagactgg
    21241 ctgacgaatc tgtggcggaa gatcctcaga aaaaatgatc gggcctgaat tcatcacgac
    21301 ggcgcgttca ataacgttgg aaagctctcg aatatttccc ggccatgacc aggcggtaag
    21361 cagtgacatt gccatcggat cgatgtcgat aatatcgcgc tgattctcac tactgaattt
    21421 ttgcaaaaag tgattagcta acagggaaat atcttcccgg cgatcgcgca gaggcggcag
    21481 tattaaatga ataacgttaa ggcgataaaa gagatcttca cggaaggtac cttcttttac
    21541 cattgcctgc aagtcgcggt tggtggcagc aatgatgcgg atatcaactt ttatggtctg
    21601 atggccgcca atccgttcaa attcccgttc ctgtagaatg cgtagtaatt tggcttgtag
    21661 taccagcggc atttcgccaa tttcgtcgag gagcagagta ccttcgttgg ctcgttcaaa
    21721 taatccctga cgcaaggttt gtgcaccagt aaatgcacct ttttcatgac caaacagttc
    21781 actttcgagc aacgattccg gcagcgccgc gcagttgact ttaatgaacg gcccctttgc
    21841 ccgccgcgaa ttgtagtgaa tcgctctggc aatcaactct ttcccggtgc cgctttcacc
    21901 gctaatcaag acgctggcct gagaaagggc aattttggcg gtgtctttgc agatgtccat
    21961 catcgccggg ctgttggtga gaatgtgccc ccattgccag ctggtgctca gtgcctggtg
    22021 cagatgacgg atctcttttt tcattgactg gagttgtaaa gcgcgctgaa cgattaaatt
    22081 caactcatcg agatcaaacg gtttaataac atagtcgaag gctccgcagc gtagcgcttc
    22141 gacggcggtt tccacttccg cataggccgt catcagaata acgggtgtcc gggtctcatg
    22201 gctgcgcatc tcctttagtg ccttgatgcc gtccatctct ggcatgcgga tatccatcaa
    22261 caccacatca gggtgaatat cggcaaacag gtgtaatgct gtgcgtccgt tgttcgcaca
    22321 atgtgtttcg aatccttgta gtgcaaaagc ggtgctcagc atacggcgaa cattatcttc
    22381 atcatccaca ataaggatgc gattaatagc agtcatacag tctgatttcc ctgcgggttg
    22441 atcggtaaaa taagcgtgaa ggttgcgccg tagcccggca aactggcgac gcgaatatca
    22501 ccctgatggg cattaatgat gcgttgactt aacgccagac caagcccggt tcctgaggct
    22561 ttggtggtga aaaagggatc gaagatcttt ttttgcagcg agagatcaat gccacagccg
    22621 ttgtcctcta tcgaaatggc ctgttgtgag tcgctgtatt gccaggtttg aatgcgaatt
    22681 ttccctcgtg cgctgatagc ctggacggca ttgatcagga tattcagtag tacctgtttg
    22741 agcagttcac gatcggcgtt aatcgggctt aattcattat ccagttcgct tatgaagtcg
    22801 acccgcgctt gtacgccggc ggtttgtacc agtaccagag tttcttcaac caatgcattg
    22861 aggctgactt gttgccattg actgtggcgt ggacgtgaaa attcgagcaa ttgctgaata
    22921 actttgttaa ttgaatcgat ttctttgagt actacggaca gatattcctg atgtattggg
    22981 tcactggttt gttggcgcaa gatctgtaca taaccacgaa tagccgttaa cggattacgt
    23041 acttcatgcg cgacgccagc catcagctca cccagtgtgg cgaggcgttc tgcttgcgcc
    23101 atgcggcgct gggtttcttt gcgggcagtt aaatcagaga aaatcaccaa agcacctatc
    23161 atttcaccgt gcgtgttatg aatacgactg gtagtgacac tgagttcaat ggtgcggtca
    23221 cgacctggaa aactgatctc cagcgccaca tgttcggtgc catgttccag cgtatccagt
    23281 actggactgt agaactgagt attgtcgaac aacatggagt aaggctgccc taccagttca
    23341 tggcgttgat agccagtgat aacttctgct gctgggttca tggtggttac atcaccctgg
    23401 cggtcaatgg caatgacgcc atcggcagcg ttttcaataa tcagatcgtt aagtgtccgc
    23461 gtttcacgca gtgcctgggc gaggttatta acactctgac tgatttgccc catttcaccg
    23521 ggcaattgtg gtaatcgagt gggaatattt tgtgccagag tcgagaggcc atcggtgatg
    23581 atatcaatat tggcgctcag gcgacgggag aaaaggacaa tcaatagcag gcttatcagc
    23641 aaaccggcgg tgagcacaat gataatcctc acatccattt tccaggcctg gcggcgaata
    23701 tcttcggtta attcattggc ccagatatag ccgaggattt caccattacg ctcaatgggg
    23761 agcattgaat tcaaaatatc gccgcgcacc tgcctgcctg aataaaccaa aggggtattt
    23821 gtacgcatga cttcgcgacc agggtgatct gcggcaatgg taacgccgac attattctga
    23881 tatagcgctg aaggcgcgta ggtgattatc gcatccagca ttttgttgta ataaccagca
    23941 ccgatgccag ggaaggcgtg agtgatattt tcggtaatgg gggcaagttc tgcatttaat
    24001 gcgcggatac gctcctcacg tggtaagtcg atgtagagat catagcgatc gcctagtgcc
    24061 tgattaagca ggttgaccac ggcagaaagt tttttctctt tttcagataa gactgctgaa
    24121 cgtccttccg tttctacgat ataaccaata gtaagcgttg ggacaatgac catcaggatt
    24181 gccatcagga tcatttgatt gcgtaagcgg cgtggataaa tccacttcat ataatgcatg
    24241 ctgaaatcca ctagtcttgt ccggtatatg acgattatca gaggttaagg tgatgatttc
    24301 tcggcggtgt atcatattcc agagaagaga gaacattgcg gtaacacgct tttaccgcta
    24361 ccttaaccac actccatcgg tcacctgagg cggagcttcg cccctttgaa ataccttgct
    24421 tcttttcgta caaccctgaa agcctcgcgc tacatgttca gagcattggc gttagtgctc
    24481 tggctgttga ttgctttttc atccgttttt tacatcgtta atgcgttaca tcagcgagaa
    24541 tcggaaattc gtcaggaatt taatctgagt tccgatcagg ctcagcgctt tattcaacgc
    24601 acctctgatg tgatgaaaga gctgaagtac atcgccgaaa atcgcttatc ggcagaaaac
    24661 ggtgtgcttt ccccgcgtgg acgagaaacg caggcggatg tgcctgcgtt tgaaccgctg
    24721 tttgccgact ccgattgttc cgcaatgagt aacacctggc gaggttctct ggagtcattg
    24781 gcgtggttta tgcgctactg gcgcgataat ttttctgcgg cttacgatct aaaccgggta
    24841 tttttaatcg gcagcgataa cctctgcatg gccaatttcg gtctgcgtga tatgccagtg
    24901 gaacgcgata ccgcgttgaa agctttgcat gaacgcatca ataaatatcg aaatgcacca
    24961 caagatgata gcggcagtaa cctctactgg atcagcgaag gtccgcgccc tggcgtcggg
    25021 tatttttacg cgttgacgcc agtttatctg gcgaaccggt tgcaggcgct tttgggtgtc
    25081 gagcagacca tccggatgga gaactttttc ttaccgggta cgttgccgat gggggttacc
    25141 attcttgatg aaaatggtca taccctgatt tcgcttaccg gaccagaaag taaaattaag
    25201 ggcgatcctc gctggatgca ggaacgctcc tggtttggct atacggaagg gttccgggag
    25261 ctggtgctga agaaaaatct gccaccctca tcgctaagca tcgtgtattc ggtgccggtt
    25321 gataaggtgc tggaacgcat tcgcatgttg atccttaacg caattttgct gaatgtgctt
    25381 gccggagctg cattgtttac tctcgcacgg atgtacgagc gacgtatttt cattccggcg
    25441 gaaagcgacg ccctgcgact ggaagaacat gagcagttca atcgcaagat tgtcgcctcc
    25501 gcgccagtgg gtatctgcat tttgcgtacc gctgatggcg tcaatatttt aagtaacgaa
    25561 ctggcgcata cctatctcaa tatgcttacg catgaggacc gccaacgact gacgcaaatt
    25621 atctgtgggc agcaggtcaa ttttgttgat gtcctgacca gcaacaatac caatctgcaa
    25681 atcagcttcg tccattcgcg ctatcgtaat gaaaacgtgg ccatttgtgt gctggtggat
    25741 gtttcttcgc gcgtgaagat ggaagagtcg ttgcaggaga tggcacaagc agcggaacag
    25801 gcgagccagt caaaatcgat gttccttgcc accgtcagtc atgagctgcg aacgccgctg
    25861 tatggcatta tcggtaacct ggatctgttg caaaccaaag agttaccgaa aggcgtcgat
    25921 cggctggtga cggcaatgaa caactcttcc agcctgttgt tgaaaattat cagcgatatt
    25981 ctcgatttct cgaagattga atcggaacag ttgaagatcg aaccgcgtga gttttcaccg
    26041 cgtgaagtga tgaaccacat caccgccaac tatttaccgc tggtggtacg caagcagcta
    26101 ggcttgtact gctttattga accggatgtg ccagtggcct taaatggcga cccgatgcgt
    26161 ttacagcagg tcatctccaa cctgttgagt aacgccataa aattcaccga taccggctgt
    26221 atagttttgc atgtccgcgc tgatggcgat tatctctcta tccgtgttcg cgataccggc
    26281 gtggggattc cggcgaaaga agtggtgcgc ttgtttgatc ccttcttcca ggtcggaacg
    26341 ggcgtacagc gaaacttcca ggggaccggt ctgggtctgg cgatttgtga aaaactgatc
    26401 agcatgatgg acggcgatat ctcggtagat tcagaaccgg gaatgggcag ccagtttacc
    26461 gtgcgtattc cgttgtacgg cgctcagtac ccgcagaaaa aaggcgtgga agggttgagt
    26521 ggtaaacgct gctggctggc ggtccgcaat gcgtcgctct gtcagttcct ggaaaccagt
    26581 ttgcagcgca gcggcatcgt cgttacaaca tacgaagggc aggaaccgac tcccgaagat
    26641 gtgttaatta ctgacgaggt agtgagtaaa aaatggcagg gtagagcggt agtgaccttc
    26701 tgtcgtcgcc atattggtat tccgctggag aaagcgccag gggagtgggt acacagtgtg
    26761 gctgccccgc atgagctacc ggcattgttg gcgcgtattt atttgatcga gatggagagc
    26821 gacgatcctg ctaacgctct gccgtcgacg gacaaagcgg tcagcgataa tgacgatatg
    26881 atgattctgg tcgtggatga tcatccgatt aaccgacgtc tgctggcaga tcagttggga
    26941 tcgttgggct atcaatgtaa aaccgcgaat gatggtgtcg atgcgcttaa tgtacttagc
    27001 aagaatcata ttgatatcgt gcttagcgac gtcaacatgc caaatatgga tggttaccgc
    27061 ttgacgcaac gcattcgtca gttgggactg acgttgccgg taatcggagt aactgctaat
    27121 gcgttggctg aagagaagca gcggtgtctg gagtccggta tggacagctg cctgtcgaag
    27181 ccggtaacgc tggatgtgat aaaacagacg ctgacggtat atgccgagag ggtcaggaaa
    27241 tcgcgggaat cgtaggacgg ataaggtgtt tatgccgcat ctggcattca gtgcaaatgc
    27301 cagatgcgac gctgacgcgt cttatctggc ctactttaat gtaatctgtt gaaataatgg
    27361 gaatcgtagg ccggataagg cgtttacgct gcatccggca atcggtgcaa atgccagata
    27421 agacactaac gcgtcttatc tggcctacag atgattagtc tttatctgcc ggacttaagg
    27481 tcacagaaga gagataattc agcagggcga tatcgttctc gacacccagc ttcatcatcg
    27541 cagatttctt ctggctactg atggttttaa tactgcggtt cagcttttta gcgatctcgg
    27601 tcaccaggaa gccttccgca aacaggcgca gaacttcact ctcttttggc gagagacgct
    27661 tgtcaccgta accaccagca ctgatttttt ccaacaggcg agaaacgctt tccggggtaa
    27721 atttcttccc tttctgcagc gcggcgagag ctttcggcag atcggtcggt gcaccttgtt
    27781 tcagcacgat cccttcgata tccagatcca ataccgcact aagaatcgcc gggttgttgt
    27841 tcatagtcag aacaatgatc gacaggcttg ggaaatggcg cttgatgtac ttgattaagg
    27901 taatgccatc gccgtactta tcgccaggca tggagagatc ggtaatcaac acatgcgcat
    27961 ccagtttcgg caggttgttg atcagtgctg tagagtcttc aaattcgccg acaacattca
    28021 cccactcaat ttgctcaagt gatttgcgaa taccgaacaa gactatcgga tggtcatcgg
    28081 caataattac gttcatattg ttcatgtaat aggctacctt gctacagcaa gctcttgaca
    28141 taactgtcaa tgtcgctgat gtatttttct attcctggaa catccttctc acgaatcaga
    28201 tgttccagcg tttcacataa ctgcttgccg ggtaccagat ttagcatggc aaatacgcct
    28261 ttaagacgat gagccgtttg ggctaacgca gcaaagtcac tggttgctgc ttcagtatac
    28321 agcctcttaa catcatccgg tactgtgtct acaaagagcg cataatagcc gctggcatgg
    28381 agttgcgcat tttcatctcc gcccagaggc gattctgtca cctcttcctg cgccagttgc
    28441 acttcaatta attgtaagac cgcttcctgc atagcgttgc tcatattgaa gttgacgcac
    28501 aattgaccag gcccaatttc ccgtacgcca gactcatcat cgcttaaaag caagccagag
    28561 gcagtaagat tagacggatt atccgttaaa aagatatcat aatcttgact aattaatctt
    28621 tcatcgggtg tgatacaggt tgcaccccaa ttttctaact ggcgagtgac aatattccga
    28681 atttctgccg aagtaacatc caccattacg cagacatcat ccagtaaacg ctcttcttcc
    28741 tcttcaactt ccgggtcagc ggcgagcatt ttgatatgca cagagtagcg tgtaccaagc
    28801 ccatcccgcg ttttgatgtt taaatgaccg cccagtttac gtgccagttg atcgctcagc
    28861 cagaatgcca gcgggtccgc ctttccataa cgatcgtttt gggtctggtt gataaacggg
    28921 aagtgcaaat tatccatttc atgaatactt acgccttctc ccgtgtccag aatgcgaaac
    28981 gtcaggcggt cttcggagga ctcatcctga tcaacctcaa gggtgatttt tcccaattgc
    29041 gttgaggtca cggcatattg catcagtagc agcaaaatac gtcgtaaggc atcgcgatcg
    29101 ccacggcgca tatcgtgtgc tttcagatga ttgttaatca gcagttgcag acctttacgc
    29161 ttgatggcag gcaacactga aggcacaact tcatcaatta aatcctgcac ggagaacagc
    29221 accgtctcac ttttccagct atcgtccgca agcatgttcg ctaactgtat ttcatcgacc
    29281 agccgcacca gcacatctgc ctgattcgcc agttgtttgc tttccggggc gttgagttta
    29341 gccgcgctct ccgccaggga ctgtgcaggt tctttcagcg catcgccaat gtttttcata
    29401 aaggtcatcc ggccctgctg gtttttctca tacagacgct gcgcctgctt gagtttcttg
    29461 tttaccagca cttcgcgatc ctgatcgcga atgatgaaaa tttgtgtgcg cggcgcgacc
    29521 tggctgcgga acatgcggat ctcatacagc tcgttattga tcgtcgcctg aataatcccc
    29581 tgatgctgtt ccgccatggt ggtgatgttt tgcagattca aatgcggcag caaatgatcg
    29641 gcaattttgt tacttatgac agtgcggttc gattcctgat cgtgaaccag caggccgagc
    29701 ggcagcagtg agactatctc ttcattgatt gcccgtaaaa tgcgcaattc gttattgacc
    29761 gccgtgttgg gtagactttc tgtactacga ctggagaaat ggcggaatgt ggtatagcca
    29821 aataacgcca gcgccagcaa accgatgttc agcagcagtg gcagcagaat gttttgcaac
    29881 gtatccagca ataaggtgcc ataaggaacc tgccagacca ggcgcatatc ggtagagttg
    29941 agtgccgagg cgatttcaat cttcgtactg ttaaagtgga tactgacgct atccgtccct
    30001 tctttctcat tatcattgtt tcccgttgcc gtcgcgtctt gctcaaggcg gaaactgtcc
    30061 agcggcatac ccggtgggat caaatcatta atcggcagat caaaagccac gaccgttgcc
    30121 agatgtcccg gctggttgaa cgtggtacgc aaggtaaagt aatgaccgtt ctgccaggcc
    30181 aggcggcgca tgttagaaaa actttcgcgt tcatcgaggg cgttggcctg ttgcaacatt
    30241 tctgcacgac gtgaatcaac aatgtcactg acggtcgatt ctttaaatcc ggaggtgaga
    30301 tctttgaggg gcagggttga gatcagcacc agactgttat cctgaccatt caggtaatac
    30361 attgaccacg gtacattttc tgcgccccac aatgtgtcca gataggtgga catccgttga
    30421 gtcatctcaa gcgttgagtt gtcgtgagag ccaaagatta acgcttccgt tttgcggcgc
    30481 ggtttttcca gatagtagac atcctgtttc aggcgcgtct cttgtaaacc ttcgccggag
    30541 gagggggagg tcgtcgcggc aatattgtcg tagatctgcc aggtcacgta acgccaggta
    30601 tcgacgcgct tttggatagc gtgggtaatg tcgacaatct ggtaactttt atctttcagc
    30661 caggcgttaa cggcgctttg taccattaca cccatcgtca ccagtaacac aatgatcaac
    30721 agtaaaaaga agcgggtaat gctccccggt aggagtgaaa agcgggtcgt ggccgttgtc
    30781 tctttctgac gcattcgtgt ttatgacctg ttaaaacttc gcgaagtgat agggcagtat
    30841 aaagggtaca gtgtgaaatt ccagactctt acctgattga tgattctcta ttctcccgtg
    30901 atatttatca tcctgcattg ggggaaatag atgtacaagc gccattttgc tgtacaaata
    30961 acctacaaaa agcccaacaa aaataattgc aaataaacaa agattgctgg aaattatgct
    31021 gat
//
LOCUS       DAPHJE010000050        30870 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPHJE010000050 DAPHJE010000000
VERSION     DAPHJE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.050"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            377..2008
                     /gene="oppA"
                     /locus_tag="RH222_002251"
     CDS             377..2008
                     /gene="oppA"
                     /locus_tag="RH222_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV3797265.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2094..3014
                     /gene="oppB"
                     /locus_tag="RH222_002252"
     CDS             2094..3014
                     /gene="oppB"
                     /locus_tag="RH222_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV3797266.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3029..3937
                     /gene="oppC"
                     /locus_tag="RH222_002253"
     CDS             3029..3937
                     /gene="oppC"
                     /locus_tag="RH222_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV3797267.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3949..4962
                     /gene="oppD"
                     /locus_tag="RH222_002254"
     CDS             3949..4962
                     /gene="oppD"
                     /locus_tag="RH222_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV3797268.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4959..5963
                     /gene="oppF"
                     /locus_tag="RH222_002255"
     CDS             4959..5963
                     /gene="oppF"
                     /locus_tag="RH222_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV3797269.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6016..6345)
                     /locus_tag="RH222_002256"
     CDS             complement(6016..6345)
                     /locus_tag="RH222_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV3797270.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6380..7840)
                     /gene="cls"
                     /locus_tag="RH222_002257"
     CDS             complement(6380..7840)
                     /gene="cls"
                     /locus_tag="RH222_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV3797271.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7983..8156
                     /locus_tag="RH222_002258"
     CDS             7983..8156
                     /locus_tag="RH222_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV3797272.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8211..9464)
                     /gene="kch"
                     /locus_tag="RH222_002259"
     CDS             complement(8211..9464)
                     /gene="kch"
                     /locus_tag="RH222_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV3797273.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9764..10060)
                     /locus_tag="RH222_002260"
     CDS             complement(9764..10060)
                     /locus_tag="RH222_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV3797274.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10284..11003
                     /gene="tonB"
                     /locus_tag="RH222_002261"
     CDS             10284..11003
                     /gene="tonB"
                     /locus_tag="RH222_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV3797275.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11043..11441)
                     /gene="yciA"
                     /locus_tag="RH222_002262"
     CDS             complement(11043..11441)
                     /gene="yciA"
                     /locus_tag="RH222_002262"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV3797276.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11546..12085)
                     /locus_tag="RH222_002263"
     CDS             complement(11546..12085)
                     /locus_tag="RH222_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV3797277.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12115..12858)
                     /locus_tag="RH222_002264"
     CDS             complement(12115..12858)
                     /locus_tag="RH222_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV3797278.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12886..12975)
                     /locus_tag="RH222_002265"
                     /pseudo
     CDS             complement(12886..12975)
                     /locus_tag="RH222_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13215..13853
                     /gene="ompW"
                     /locus_tag="RH222_002266"
     CDS             13215..13853
                     /gene="ompW"
                     /locus_tag="RH222_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV3797279.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13913..14419)
                     /locus_tag="RH222_002267"
     CDS             complement(13913..14419)
                     /locus_tag="RH222_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV3797280.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14465..14965)
                     /locus_tag="RH222_002268"
     CDS             complement(14465..14965)
                     /locus_tag="RH222_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV3797281.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(15051..15230)
                     /locus_tag="RH222_002269"
     CDS             complement(15051..15230)
                     /locus_tag="RH222_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV3797282.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(15611..16417)
                     /gene="trpA"
                     /locus_tag="RH222_002270"
     CDS             complement(15611..16417)
                     /gene="trpA"
                     /locus_tag="RH222_002270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV3797283.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(16417..17610)
                     /gene="trpB"
                     /locus_tag="RH222_002271"
     CDS             complement(16417..17610)
                     /gene="trpB"
                     /locus_tag="RH222_002271"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV3797284.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(17622..18983)
                     /gene="trpCF"
                     /locus_tag="RH222_002272"
     CDS             complement(17622..18983)
                     /gene="trpCF"
                     /locus_tag="RH222_002272"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV3797285.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(18984..20579)
                     /gene="trpD"
                     /locus_tag="RH222_002273"
     CDS             complement(18984..20579)
                     /gene="trpD"
                     /locus_tag="RH222_002273"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV3797286.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(20579..22141)
                     /gene="trpE"
                     /locus_tag="RH222_002274"
     CDS             complement(20579..22141)
                     /gene="trpE"
                     /locus_tag="RH222_002274"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV3797287.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(22162..22255)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(22232..22276)
                     /gene="trpL"
                     /locus_tag="RH222_002275"
     CDS             complement(22232..22276)
                     /gene="trpL"
                     /locus_tag="RH222_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV3797288.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            22414..23295
                     /gene="yciV"
                     /locus_tag="RH222_002276"
     CDS             22414..23295
                     /gene="yciV"
                     /locus_tag="RH222_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV3797289.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            23292..23912
                     /locus_tag="RH222_002277"
     CDS             23292..23912
                     /locus_tag="RH222_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV3797290.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            24013..24885
                     /gene="rluB"
                     /locus_tag="RH222_002278"
     CDS             24013..24885
                     /gene="rluB"
                     /locus_tag="RH222_002278"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV3797291.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(24925..25515)
                     /gene="cobO"
                     /locus_tag="RH222_002279"
     CDS             complement(24925..25515)
                     /gene="cobO"
                     /locus_tag="RH222_002279"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV3797292.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(25512..26270)
                     /locus_tag="RH222_002280"
     CDS             complement(25512..26270)
                     /locus_tag="RH222_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV3797293.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEVTGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            26490..27539
                     /gene="sohB"
                     /locus_tag="RH222_002281"
     CDS             26490..27539
                     /gene="sohB"
                     /locus_tag="RH222_002281"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV3797294.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(27575..27826)
                     /locus_tag="RH222_002282"
     CDS             complement(27575..27826)
                     /locus_tag="RH222_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV3797295.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            28206..30803
                     /gene="topA"
                     /locus_tag="RH222_002283"
     CDS             28206..30803
                     /gene="topA"
                     /locus_tag="RH222_002283"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV3797296.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         7852 a         7522 c         7876 g         7620 t
ORIGIN      
        1 aaggcatgga tgcaagctat agattctgat acggtcaata aaagagaatt gcttaacaat
       61 tttgcaaaat gtattggcga gtaagaaccg catttggtac tttccgggca accgccagac
      121 gattctttat tggtaatgag aataattaac aattaaagag cgtcgcgaaa gaataatgtg
      181 tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat caccagaata
      241 aataaagtcg gtgatagtaa tacgtaacga taaagtaacc tgacagcaga aagtctccga
      301 gcctgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta attataatga
      361 gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag ctggcgttct
      421 ggctgcgcta atggcaggga atgtcgcgct ggcagctgat gtacccgcag gcgtcacact
      481 ggcggaaaaa caaacactgg tacgtaacaa tggttcagaa gttcagtcat tagatccgca
      541 caaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag gcttactggt
      601 cagcgatctt gacggtcatc cagcacctgg cgtcgctgaa tcctgggata ataaagacgc
      661 gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca cgccagtcac
      721 agcacaagac tttgtgtata gctggcaacg ttctgttgat ccgaacactg cttctccgta
      781 tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg aagggaaaaa
      841 accgattacc gatctcggcg tgaaagctat tgatgatcac acattagaag tcaccttaag
      901 tgaacccgtt ccgtacttct ataaattact tgttcaccca tcaacttcac cggtgccaaa
      961 agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatcg tcaccaacgg
     1021 tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac gcagcccgac
     1081 ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta ttgcttctga
     1141 agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacttata acaacatgcc
     1201 gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg ttgatccata
     1261 cctgtgcact tactattacg agattaacaa ccagaaaccg ccattcaacg atgtgcgtgt
     1321 gcgtaccgca ctgaaactag gtatggaccg cgatatcatt gttaataaag tgaaagcgca
     1381 gggcgacatg cccgcctatg gttacactcc accgtatact gatggcgcaa aattgactca
     1441 gccggaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa aactgctggc
     1501 tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgttgtata acacctccga
     1561 tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca ttggtgtaaa
     1621 cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc agggtacttt
     1681 tgatgtggcc cgtgcaggct ggtgtgctga ctacaacgaa ccaacttcct tcctgaacac
     1741 catgctttcg aacagctcga tgaataccgc gcattataag agcccggcct ttgacagcat
     1801 tatggcggaa acgctgaaag tgactgacga ggcgcagcgc acagctctgt acactaaagc
     1861 agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg tgaatgcgcg
     1921 tctggtgaaa ccttgggttg gtggctatac cggcaaagat ccgctggata atacctatac
     1981 ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggagt gtcctgctcc
     2041 acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg gcaatgttaa
     2101 aatttattct acgtcgctgt ctggaagcga ttccgacgct atttattctt attactattt
     2161 cgttctttat gatgcgcctc gcgccgggaa gcccttttac cggcgaacgt actttaccgc
     2221 ctgaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc atgacacagt
     2281 atttcagcta cctgaaacaa ctggcgcacg gtgatttcgg tccatcgttt aaatataaag
     2341 attattcggt caatgacctg gtggcatcca gttttcccgt ttctgccaaa ctgggagccg
     2401 cagcattttt ccttgcggta atactgggtg ttagtgctgg cgttattgcc gcattaaaac
     2461 aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt gttatcccca
     2521 gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat tggctgccgg
     2581 gcggtggctg gaatggtggg gcgcttaaat tcatgatatt gccgatggtg gcgttgtcac
     2641 tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgaa gtattacact
     2701 ccaattttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc attttacgcc
     2761 acgcattaaa acctgctctg ttacccgtgc tctcctatat gggccccgca tttgtcggca
     2821 ttattaccgg ttctatggtt atcgaaacca tttatggttt gccggggatt gggcaactgt
     2881 tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagcctgacc atcctggttg
     2941 gtgctttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt atcgacccga
     3001 aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca gcgagacgct
     3061 ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg acgcacgtcg
     3121 acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc tgatcgcgtt
     3181 atttgtaatc ctggcaccga tgctttcgca gtttgcctat gacgatactg actgggcgat
     3241 gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact catccggtcg
     3301 cgacctgctt gtgcgcgttg cgattggcgg gcgtatctca ctcatggtcg gtgttgctgc
     3361 ggcactggtg gcagtggtcg tcgggacact ttatggttcg ctttccggtt atctgggcgg
     3421 taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc cattcatgtt
     3481 cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc ctgctgattt tcgtggcgat
     3541 aggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc tgagtctgaa
     3601 gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacatcgg gcattgttat
     3661 tcgccacatt gtgccgaatg tactcggtgt ggtggtggtc tacgcatcac ttctggtgcc
     3721 cagcatgatc ctctttgaat ctttccttag cttcctgggg ttgggtacgc aagagccgtt
     3781 aagcagctgg ggtgcattgc tgagtgatgg cgcgaactcg atggaagtct ctccatggtt
     3841 attgttgttc ccagcgggat tcctcgtggt gacgctgttt tgtttcaact ttatcggcga
     3901 tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat gagcgtaatt
     3961 gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa cgtgaaagat
     4021 ttgcgtgtca cctttagtac cccggacggc gacgtcacgg cggtcaatga tttgaatttt
     4081 tccctacgtg ccggagaaac gctgggtatt gtaggtgagt ctggttcggg taaatcgcaa
     4141 actgcatttg cgttgatggg cctgttggct gccaacggac gtattggcgg atcggcaacc
     4201 ttcaatgggc gtgaaatcct caatttgcca gagcatgaac tcaataaact gcgcgctgaa
     4261 caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat gcgcgtcggt
     4321 gagcagttaa tggaagtgct gatgctgcat aagaacatga gcaaagctga ggcgtttgaa
     4381 gagtcggtgc ggatgctcga tgcggtaaaa atgccggaag cgcgtaaacg catgaaaatg
     4441 tacccgcacg aattttctgg cggcatgcgt cagcgagtca tgattgcgat ggcattgcta
     4501 tgtcgaccta agctactgat tgcggatgaa cccactactg cgctggacgt caccgtacag
     4561 gcgcagatca tgacgctatt aaatgaactg aagcgggaat ttaataccgc catcattatg
     4621 atcacccacg atcttggcgt ggtggcgggg atctgtgaca aagtgctggt aatgtacgcc
     4681 gggcgcacga tggaatatgg caatgcgcgc gatgtctttt atcaacccgt tcatccttat
     4741 tctatcggtt tgctcaacgc ggtgccgcgt cttgatgcgg aaggtgaaac aatgttgacc
     4801 atcccgggta atccgccaaa cttgctgcga ttaccgaaag gttgcccgtt ccagccacgt
     4861 tgtccacatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt tacgcctggc
     4921 cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta actgaaggaa
     4981 gaaaagtcct ccttgaaatc gccgatctta aagtgcactt tgaaatcaaa gatggcaaac
     5041 agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtcact cttcgcctgt
     5101 atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc acctttgctc
     5161 gcgccatcat cggtttggtc aaggcgaccg acggtcatgt tgcctggtta ggtaaagagt
     5221 tgctgggcat gaagcccgat gaatggcgtg ccgttcgcag tgatattcag atgattttcc
     5281 aggatccgtt ggcatcgcta aacccgcgta tgaccatcgg cgagatcatc gctgaaccac
     5341 tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg aaggcgatga
     5401 tgctgaaagt cgggttattg cctaacctga ttaaccgcta tccgcatgag ttctccggtg
     5461 ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag ctgattatct
     5521 gcgatgagcc ggtttcggcg ctggacgtgt caattcaggc gcaggtggtc aacctgctcc
     5581 agcagctgca acgtgagatg ggattgtcat taatttttat cgctcatgac ctggccgtgg
     5641 taaaacacat ttccgatcgt gtgttggtga tgtatctcgg ccatgcggta gaactgggga
     5701 cctatgatga ggtctaccac aatccactac atccttacac cagggcattg atgtcggcag
     5761 tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg gaaggggaat
     5821 taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgc ccgattgccg
     5881 gtccggagtg cgccaaaaca cgtcctgttc tggaggggag tttcagacac gccgtttctt
     5941 gcctgaaagt cgatccgctt taaacacaat aagggctgac agttgtcagc cctttttcac
     6001 gctaaaagcg attatttatt cccgccagat gatatggcaa agtttgtgat ctttttcgcg
     6061 gcataacaga atgcgggcga aaacatcgtt gatttcacca tcttcactgt ccgccagacc
     6121 aatcaccact tcggcaaaaa agtctgggtt caaatcgaaa tcaacatgtt cctgccagtc
     6181 ttccgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat tgaacagcag
     6241 aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt aggcctgctc
     6301 aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca ctacctgttt
     6361 aacctctgtt ggcgacgttt tacagcaacg gactgaagaa gtaaaacagt cgctcggcga
     6421 cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacgt gaacgcgaaa
     6481 tataatcgtc ctgaacggcg gcaaggtctg caccaaaacc tttatcgtcg attgccaggg
     6541 taatctcgaa attcagccac agactacgca tatcaaggtt aacggtgcca accagactta
     6601 gttcgccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac tgataaattt
     6661 taaccccagc agcaagaagt tccgtaaaga atgcgcgact ggcccagccg accagcatcg
     6721 agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc gccgtgcaaa
     6781 tcgcatgaag taaatcatcg cttggcacaa agtagggcgt ggtcatgatc aaatattcac
     6841 gcgccgaata agccgcagtc aataatgcct ggtgaatgag atcttccgga aagccggggc
     6901 cagaagcaat tgtgtgaatg gtgtgaccgc tggcctgttc aaacggcata atattgacat
     6961 ctggtggtgg cggcagaata cgttttccgg tttcaatctc ccagtcgcag gaataaataa
     7021 tccccatcgc ggtggcgatg gggccttcca tacgcgccat cagatcaatc cattgcccta
     7081 cgcccgcatc ttgtttgaag tagcgaggat cgaccatatt catgctgccg gtgtacgcga
     7141 tgtaattatc gatcatgatc atcttgcgat gttggcgcag gtccatacgg cgtaaaaaca
     7201 cacgcatcag attgaccttt aaggcttcga ccacttcaat accggcatta cgcattagct
     7261 cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc aatcggcaat
     7321 gaataccacg ccgtgcagcc gccattaatg attcagccac ctggtccgcc atgccgccgg
     7381 gctgccagat ataaaacacc atctcaatat tatggcgcgc gagctggatg tcgcggatta
     7441 acgcctgcat cacatcatct gactcggtca tcagttgtag ctgattccct ttgaccccag
     7501 cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg ctattttctt
     7561 cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg gaaggccaca
     7621 tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg gcaagatagg
     7681 caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc gcggagggaa
     7741 ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac cagtatccca
     7801 gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa tccttttgaa
     7861 agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac gcgggaaaag
     7921 cactggtcag cgtcgggggt gggggtataa tgaccattct gttattgcat agagtagtta
     7981 acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag gctagccgac
     8041 gtaaatcgcg ttggcttgag gggcaatcgc gccgaaatat gcgtatccac agcatcagga
     8101 agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat atctaaatgt
     8161 actgggcacc attgcggtgc ccaataagtt tactcttccg tctttaaact ctatttttgc
     8221 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa aataccatga
     8281 cctgagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
     8341 cgcgccagaa tttcgctgcc aaacagttgc ggtgagagaa tgatatccgg atgtaccatc
     8401 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
     8461 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
     8521 atggcccggc aacgatcgat tcccgctttc tttaatactg aactgtcatt actgtcaccg
     8581 gggataacat cagcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
     8641 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcaggat cgtattgatg
     8701 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
     8761 ccttttacaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
     8821 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
     8881 gaaacaggga caatatcgcc atagccgacg gttgacatgg tttctatcga aaaatagaac
     8941 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtag
     9001 gttgagtaaa acagtaatgt cgtgaaacta ataaatgcaa aaattgtccc ggctgcggcg
     9061 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
     9121 ccaatactaa atttcagcca gggataaaaa tgcagggtgt aaattagcgc tatcaacaac
     9181 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
     9241 acaagaaaga cgccgagcat aaatagtggg gcgtttgcca gcaaggagag atccaactct
     9301 gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata gattgtttta
     9361 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg aagggtcacc
     9421 cataaatttg ttgctgtttg tttgaatgta gcccagtgac tcactgatac ctctgtaacc
     9481 acaattttgt ttaattatct tagcgtgcac attttctgaa catacatgca gcgcgtgaat
     9541 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggatttgag cgagcaactg
     9601 tacctcagga aaggtcattt ttcctgaata tgctcacatc atataaagaa atacagataa
     9661 agttattatc tgcttgtggt agtgaatgca ctgaccggct ataaggaaag gccaaacaag
     9721 aacacggttg caaaaaccgt gcccttaaat attgaatctc tattcagaac actttcttaa
     9781 atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc
     9841 aggcctgagc tgcttccagc gattcaaatt cagcaataac ggttgagcca gtaaatcccg
     9901 cagcccctgg atcgttactg tctaccgctg gcattggacc agctgtcaac aaacgacctt
     9961 catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg cgtttttcga
    10021 ggctatcagc tttgtcttga gcgtaaataa catacaacac gggcacaact ccttgttcgg
    10081 gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt
    10141 tgttaagacc atgcataaat taaggtcaat tacgccaaaa atgacatttt cactgatcct
    10201 gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgaga cctggttttt
    10261 ctactgaaat gattatgact tcaatgaccc ttgatttacc tcgccgcttc ccctggccga
    10321 cgttactttc ggtctgcatt catggtgctg ttgtggcggg tctgctctat acctcggtac
    10381 atcaggttat tgaactacct gcgcctgcgc agccaatttc tgtcacgatg gttgcgcctg
    10441 ctgatctcga accgccacaa gccgttcagc cgccaccgga gccggtggta gagccagaac
    10501 cggaacctga gccgatcccc gaaccaccaa aagaagcacc ggcggtcatt gaaaagccga
    10561 agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt acaggagcag ccaaaacgcg
    10621 atgtcaaacc cgtagagtcg cgtccggcat caccgtttga aaatacggca cccgcacgcc
    10681 tgacatcaag tacggcaacg gctgcaacca gcaagccggt taccagtgtg gcttcaggac
    10741 cacgcgcatt aagccgtaat cagccgcagt atccggcacg agcacaggca ttgcgcattg
    10801 aagggcaggt taaagttaaa tttgacgtca cgccggatgg tcgcgtggat aacgtacaaa
    10861 tcctctcagc caagcctgcg aacatgtttg agcgtgaggt gaaaaatgcg atgcgcagat
    10921 ggcgttatga gccgggtaag ccaggcagtg ggattgtggt gaatatcctg tttaaaatta
    10981 acggcaccac cgaaattcag taagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
    11041 tattactcaa caggtaaggc gcgaggtttt ccttcaggat caaccgcgac atacttaaat
    11101 aatgcttctg tcgctttata gcgttgcccg attggttcag acgctacttt tttcacccac
    11161 acttcaatat taatgctgac cgatgtcgtc cctttctgga cacagcgtgc atagcagcac
    11221 accacatcgc cgaccgcaac cggccgtaag aaagtcattc cttcaacccg cacagtcact
    11281 acgcgaccgt gggcaatttc tttcgccaga atagcgccgc caatatccat ttgtgacatt
    11341 aaccaaccac caaagatgtc accattggca ttggtatcgg cgggcatggc taaagtacgt
    11401 aaaacaagat cgccctgagg gacgttatgt gttgtagaca tggtaaaacc gacttaaaag
    11461 gaaaatcaca ggcgcgatgc tactatgatt ttcctgagga gaacagagga gatacagcgc
    11521 cagccccgaa ggactggcag tctggttagg atttatcttc ctgcggcatg tggcggtaga
    11581 tatagatacc gcttaacaat gtgaagatta aggtaagggc ggtcaggcca aagactttaa
    11641 agttgaccca aatattttgc ggcagccaga atgcgatgta gatgtttgcc agaccgcaaa
    11701 ggataaagaa aacagcccaa gccagattta gcttcgacca taccggttgc ggcagcgtga
    11761 gttctttacc cagcatccgc tgaattagcg gctttttcat cacccattgg ctgactaaca
    11821 gggctcccgc aaacagagca taaatgactg taaccttcca tttaataaac tcatcattgt
    11881 ggaagaacag cgtcaagcca ccgaagacga ccaccagaac aaaagtgatc agggccatct
    11941 tctcaacctt acgaaagcga acccagctat agataagcac aatcgccgtg gcgacgatca
    12001 gcgccgcagt agccgcatag atgtcataaa tcttgtaaaa cgcgaaaaag acaaccagcg
    12061 gtaaaaaatc aagaaactgc ttcattttac gattccgtaa tcaaatgctt aaggttattg
    12121 gcgaatcaac atatacaggc ggaacagata gatgagcaat atggcagaaa tcaggttgct
    12181 caaggtgttc gccagtacgg caccaatttc cggggttaat gcggcaaaag aagaggcaaa
    12241 aagcagcaac agtgtttttg ccagcaacca gctcagtact gcgggtgcca ccagacgcat
    12301 attcgcccaa gtcagccgca tactgctacg catcgaggca aaaatgccca ttttgtcctg
    12361 taccagcatc accggtgcca gggccagtaa aatggccatg ataattcccg gaacgaccac
    12421 cagcatgatg ccaatctgta ctaaaagggt agtcagaaaa atcagaataa ataacttggg
    12481 caatatcggc gcactggcac caatagctcg cagcgcactg actctctgac ccgcagacac
    12541 cagctggata attaatatta cgcctccggc gagaatggcg ttaccgatta atcctgaaaa
    12601 agtggacgcc gctgaagcct gcagcaaaat ttgttgctgt tccggtgaca tattctgaac
    12661 caggtcgaac aacccactac tgccgctaac gggcacgccg tcattgagct gcgcaagctg
    12721 tgcatcactg ggtgagaaaa catgccctaa caccactgtg ataaacgcac atagcaacga
    12781 taccaacaga atggtcataa attgattacg gaagaaattt ccggtgtcac ggtatacgga
    12841 ctgcgccgtg atagacatgc actctccttg agttttatgc aggtgttaat tagcgggcaa
    12901 ttgtaccctg gttaagcctc gggtggcagc atcaggtctt gtatggaaaa gcatatcttt
    12961 gtaaagcgga ggtaattgct ggccttcagg cgtgggcaaa ccgcggcaag tggcgatcga
    13021 gttaatattt gcgtagagaa aatatttaaa aattgattta aatcacatta accaggattc
    13081 tcaatgcaac ttctaaatta atccagatca ataaatggtg aattatcata tgtaatgtga
    13141 tctatgtagg atcatttgtt actccaatgt aggtatattc gtcacgtttt tataaccata
    13201 acgacggagc ggatatgaaa aagttaacag tggcggcttt ggcagtaaca actcttcttt
    13261 ctggcagtgc ctttgcgcat gaagcaggcg aattttttat gcgtgcaggt tctgcaaccg
    13321 tacgtccaac agaaggtgct ggtggtacgt taggaagtct gggtggattc agcgtgacca
    13381 ataacactca actgggcttg acgtttactt atatggcgac cgacaacatt ggtgtggaat
    13441 tactggcagc gacgccgttc cgccataaaa tcggcacccg ggcgaccggc gatattgcaa
    13501 ccgttcacca tctgccgcca acactgatgg cgcagtggta ttttggtgat gccagcagca
    13561 aattccgtcc ttacgttggg gccggtatta actacaccac cttctttgat aatggattta
    13621 acgatcatgg caaagaggcg gggctttccg atctcagtct gaaagattcc tggggagctg
    13681 ccgggcaggt gggggttgat tatctgatta accgtgactg gttggttaac atgtcagtgt
    13741 ggtacatgga tatcgatacc accgccaatt ataagctagg cggtgcacag caacacgata
    13801 gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccaca
    13861 aaaaaacgac cccgtaatat acggggtcaa taaggacatg gcataaagtg tattatttct
    13921 tcgcttctac gccgtcagtt tcagagcgaa ttaaaaattt ctcagttgtt tgcggaatat
    13981 gctgaatcag ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atggttggaa
    14041 ttgaagctgt atcaccggca ttttttgctg ctgctaatag tgaggtgtaa caggcgattt
    14101 caaattgctc gaagacatat ccgctaatag agcctttgac tatttcatca gaagggaata
    14161 taccaccgat tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac
    14221 gtgaaatgtc attacgatca agaatagttt ccagttgaac aatctggttt ttggtttcac
    14281 taagatgttg ttcaatacga gcgcgtagtt caggataatt atctatacgg ctggccatgg
    14341 attcaagcat agattcggct tgcttttcca ttgcgtgggc gtcacgtaac cagtcatgat
    14401 aatgttcaat acgattcatg aaaatgtcct ctttataaaa tgactaaaag ttaaagttca
    14461 tatttcaggc tttattttcg gctttcttat ttacgttatt aagggccaga tcagtcagtt
    14521 tgatgtcggt ggccttttct tcttccaggg tctctttcag aagcttcgct gctttacgat
    14581 aacctaattg ttcagccagc gtcgctaatg tcccgtaact ggcaatctca taatgctcga
    14641 ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc gtttttctcg gtactttcga
    14701 tgacctcatt agcttcttca ataagacctt ccattgccac acatttcatg cgcttaattt
    14761 tcagattcga ttctgattcc acaacttgat caatacgttc aatctgtcca tgtgtttcct
    14821 cgaggtgcgc atgaaaagcc tgacttaatt tttcgtttga tgttgctctt gcgagttttg
    14881 ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc tgaaagcagg tgaataaata
    14941 catcttcaat ggtcttcata ttcatatttt tctccagtga aatcactgcg agccatacgg
    15001 cccgcagatg caattatctt tatcagctga tattaataat taacggtgaa tcaggatttg
    15061 cctgatttat taccaccgct ttgttgaccg ccttttttac ccgcttcaga tgcgcgttgc
    15121 ggatcatttt taaaattacc gccgctatgc tgaccgcctt tacggcctgc gtcggatgcc
    15181 ttctcacggt cttcggcgaa atttcctgaa ccaccacgat gttcggccat gttatttctc
    15241 ccgttgcgtt gcattgtttc attaatatga gtgttgtgtg tcgacactca ttaaaattag
    15301 tcgctaatga gaattagtca aattaagcgc aacgagaaaa tagagggaaa atatattttg
    15361 aggaacattc tggatatatt aacaattacc tgaggaataa gtgacttaga gaaaattatt
    15421 gattttactg gtgttatgtt gcgggattaa tttgtttgat tatattactg ttgggcggaa
    15481 aaatgacgta agttgacgtt cgaccggggt aagcgaaacg gtaaaaagat aaatattaaa
    15541 tgaatttagg atttttccgg tttcattaaa gaaagttaaa atgccgccag cggaactggc
    15601 ggctgtggga ttaactgcgc gtcgccgctt tcatcggttg tacaaaaact ttcagtgccg
    15661 ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacaatg gccgaaccag
    15721 aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac ctgatccggg gcggaaatac
    15781 caaacccctg caatggaggt gcagcgttgt actctttcag cttcgcaacc agatgattga
    15841 ggggtaacgc ggcgcggttt tctgcgccgg tcacgcccgc tcgtgacagc aaataggtgt
    15901 aaccacgacc gtaagaggct atctggcgca gcaggtcgtc atcggcattt ggcgggcaga
    15961 tgaagatagg tgcgacatta tggcgcaacg cggcctggcg gaagggcgcg gactcttcaa
    16021 ttggcacatc ggcaaccagc accgaatcga cgccgacttt ctcgcactgg gcataaaact
    16081 catcaatgcc tttgttaaac accagattgg catacatcaa caggccaatg ggaatggtcg
    16141 ggtgtttctg gcgaatcagt gccagcattt caaaacattg tgccggagtc acacctgccg
    16201 caaaggcgcg cagagtggcg ttttgaatgg tcgggccatc cgccagtggg tcggagaagg
    16261 ggatacctaa ctccagcgcg tcagcaccgg cttcaattag cgtatcgata attttcaatg
    16321 actgctcaat gcccggatca ccgagcgtga cgaaaggaac gaatgcgcct tctttgcgct
    16381 ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc gtgctttcaa
    16441 aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa ccaccagtag
    16501 ctgctcttta tccgggtttt cgcgcatcat tttcaacgca tgggccaggg cgtgggagga
    16561 ttccagcgcc gggatgatcc cttcgtgcag gcacagcgtt ttgaaggctt caagggcttc
    16621 atcatcggta atagacacgt aatcagcgcg tccagtgctg ttaagatacg cgtgttgtgg
    16681 gccgacagac gggaaatcca gtccggcgga gatggagtaa gattcttcaa tctgcccgtc
    16741 ttcggtttgc atcatcggcg ctttcatacc gaaatagatg cccacgcgac catgttttaa
    16801 cggtgcgccg tgctcgccag tttcgatacc gtgaccacct ggctccacac caatcaggcc
    16861 gacgttggtt tcattgatga aatcagcaaa catgccgatg gcattcgaac cgccgccaac
    16921 acaggcgata acggcatccg gcaggcgacc ttctctttcc agaatctgcg ctttggtttc
    16981 ttcgccaatc atccgctgaa actcacgcac aatggtcgga taaggatgcg ggccagctgc
    17041 ggtgcccagc atatagtgcg cggtttcgta actaccggac cagtcgcgca gcgcctcgtt
    17101 acaggcatct ttcagcgtcg cggaaccgct atgcaccggg atcacttccg cacccattaa
    17161 gcgcatacga aaaacgttag gcgactggcg ttctacgtct ttggcaccca tataaatacg
    17221 gcatttcagg ccgagcaggg cgctggcaag ggccgacgcc acgccatgct gaccggcacc
    17281 ggtttcggcg atgatttcgg ttttacccat ccgcttcgcc agtaaagcct gaccgagcac
    17341 ctggttagtt ttatgcgcgc cgccgtgcag caaatcttca cgcttgagat acagagtggt
    17401 gttcgtcccg gctgtaatgt tctggcattt ggtcagcgcg gttggacgcc cggcatagtt
    17461 tttcagcagg tcgttgaact gagcctgaaa ttcaggatct ttttgcgcac tgacaaaagc
    17521 ttcttccagc tggcgcagag caggcatcag gatttgtggc acgtacatgc cgccaaactc
    17581 accaaaatag gggttaagta atgttgtcat tgttcctttc cttaatatgc gcgcagcgtc
    17641 tggaaaaccg aggccaaaag acgtgcgtct ttgatgcccg gttgcgactc tacagcagaa
    17701 ttaaaatcaa gtccggcgca gccggtttgt gccgcttcca cgcagttatc tgcgcctaag
    17761 ccccccgcca gcagaacgtt gccaagcgat tgaccattta atagtgacca gtcgaaacgt
    17821 tgcccgcttc caccctggcc gttgtctaaa acatatttat caacgtgctg aagctcgcgg
    17881 gcgggcaggg tttcaccgac gcttaatgct ttccagatgg caacgtgtgc tggcagagct
    17941 tcacgcagcg tatcgatata cagctgatct tcattaccat gcagttgcac tgccgccagc
    18001 gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa cacgccaaca
    18061 tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac gcaacgcggt
    18121 gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc tgctttagca
    18181 tcttgcccac gcgtcaggcc acatacttta ttctcaccca gcaacacccg gcgcacggcg
    18241 gcgtgcaaat cgtcatgggc catcaacgcc gaaccaatca gaaaaccgtt agcgaagtgg
    18301 cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac cgttacgttg
    18361 tgccccagtt tcggcgcaag ctcgcgggta cggttgagat caatcgacaa atcacgcaga
    18421 tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa tggcacgctc cagttcctct
    18481 tcattactga cttcggtcag cacacccatc tccagactgt gggcgacggc ggcaagctgg
    18541 cgatattgtt cgtcatccag tactgaaagc attaataagc aggcatcagc ctggtaatag
    18601 cgcgccagat agatctggta aggatcgata atgaagtctt tacataaaat cggctgcggg
    18661 gcgatttggc tgacgatggg gaggaaatca aagctcccct gaaaatattt ctcatcagtc
    18721 agcaccgaaa ttgccgaagc gtaatgttta taaatggcgg caatgcgtgc tggatcgaaa
    18781 tcatcacgga tcacgccttt tgacggcgac gctttcttgc actccagaat aaacgccgtg
    18841 cgcgcaccct gtagcgcatc ataaaaatgt cgcgtgctcg gctgaacctc attctgaaaa
    18901 ctggccagcg gttgctgctg tttgcgggtt tctacccaaa tcgccttgtc tgcgacgatt
    18961 ttcgctaaaa cggtttgcat catttaccct cgtgccgcca gcgcggtaac tctgtcgtaa
    19021 gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag atcttcatgg
    19081 ccatgcaggc gcattaacat ggcgacgttc gcagcgacgg ctgcttcatg ggcggcgtcg
    19141 cctttacctt gtaacaaacg tgttaaaatg tcacggtttt cttccggtgt tccgcctgcc
    19201 agttgctcct ggtggtaggg tgtcaggcca aaatcttcag cggtcaattg atagctctta
    19261 atttcgccgt catgcagctc ggcaacgatt gtcggcgcgt gtaatgaaac ttcatccatc
    19321 ccgccgctgt gcaccaccgc cgcgcgttga taccccagca cgcgcaaggt ttcggcaatc
    19381 ggcagcacca gttccggact ataaacacca attaacgcca gcggcggatg cgccgggtta
    19441 atcaatggcc ccagcacatt gaacagggtg cgggttttta gttgctggcg aaccggcatt
    19501 gcatggcgaa atccggtgtg atatttcggt gcaaagagga aacatacgcc taactcatcc
    19561 agcgcctggc gcgatttatc ggcgttcata tcaagattaa taccgaacgc cgccagcaga
    19621 tcggacgaac cagatttact ggagacgcta cggttgccgt gtttcgccac tttcagccca
    19681 caggccgcgg cgacaaacgc actggcggta gaaatattga tactgttgct gccgtcaccg
    19741 ccagtaccga cgatatcagc aaacagataa tccgggcgcg ggaacggcgc tgcgttttcc
    19801 agtagcgcgg ttgctgcccc ggcgatctcg ttcgggtgct caccgcgaat tttcatgctc
    19861 accagcgccg ccgccagttg ttccggcttc agctcgccac gcaccaccgc tgaaaacagc
    19921 tggtggcttt cttgttggct aagcgtctgc gcctgataca gtttttccag aatcggttgc
    19981 agcgtgttgg ctggctctag tttctgctgc gcccaggcca gcgtttgttc cagcaggcga
    20041 gcgccctggg tggtgagaat ggattccgga tggaactgga atccacaaac gcgatccgca
    20101 tcgtgacgta ctgccatcac catgccatta aaatgggcgt tgatggttaa accggccgga
    20161 atgttactgc caaccagcga gtgataacgc gccaccggca gcgggtttgt taatccggca
    20221 aacatcgcct gaccgtcatg ttcaatgctc gacgctttac cgtgaagaat ttcgcccgcc
    20281 tgaccgacat agcccccgta agcttcgaca attgcctgat gtccgaggca aatgccaata
    20341 atgggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc ttcgctcggc
    20401 acaccggggc caggagaaag catcagtacc ggattgctca tggtcgccag gcgttcaatt
    20461 aaggtctgcg ccggaatatg gttgcggtaa atcaccacgt tatgcccatt gctgcgcaac
    20521 tgatctgcca ggttgtacgt aaaagagtcg atattatcga gcagcagtat gtcagccatc
    20581 agaaagtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg cgggctttat
    20641 tacgggtttc gtcggcttcc gactgcggaa tagaatcaag gactacgcca gcaccggctt
    20701 gcacggtggc gataccgttt tccaccagcg ccgagcggat cacaatgcag gtgtcgagat
    20761 cgccgtgcgc ggtgaaataa cctaccgcgc cgccgtagct gccgcggcga cgaccttccg
    20821 cctcggcaat taactgcatg gcgcgcactt tcggcgcacc gcttaacgtc cccatattca
    20881 tacaggcgcg gtaagcgtgt agggcgtcga gatcgtggcg cagctcaccg accacgcggg
    20941 agaccaggtg catcacgtaa gagtaacggt caactttggt gaggtcggcg acgtagcggc
    21001 tgccgggggt gcaaatgcgt gccagatcat tacgggcaag atccaccagc atcagatgtt
    21061 cagaaagctc tttatgatcg gtacgcattt ccagttcgat gcggctgtcg aggtctctgt
    21121 ccagcgagcc atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg tagatctcaa
    21181 tctggcggct ggtggcgtca tacttgagcg aactttccgg cgacgcgcca aacagggtga
    21241 aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc agcacgtaat
    21301 aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc acctggaaaa
    21361 tttctccagc gcgaatcgct ttttgcaaca aacgcactac gccaccgaac tcttcatcgc
    21421 tctgattaca ttcacaacgc atatgcggca cggaaaccac cggcagcggc ggcgcggctt
    21481 cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt tcttcttcat
    21541 tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca atcaccatca
    21601 gagtttcagc gagataaaaa cagaaatcag ggcagttatt ttccgctgac agttgcggta
    21661 aatcttcaaa tcccgccaca aggtcataag agaacaggcc gccgaagaac attgcttctc
    21721 gttcttcctt cggtacattc aacagattct gcaataaacg gaaagcgtca aaaaccgaaa
    21781 gggagcataa gcgagcgtct tcatccagca gtggactgac aggggggaag cgcagcacac
    21841 ggcagtttgg taattgttca ttttccacac ccgcaggcag ggcgttatcc agtagtgtca
    21901 gcagggcttc gccgttgccg gaaagtgcct ggattgtgac agtgtcacct aaagctgtaa
    21961 tgcgcagcgc actgtctacc agcagcaggc tttttaaatc atctttgctg tcgatatctg
    22021 cggattccag cagcagcgtt gccggacgat ccccacacaa ctggtgaaaa agcgcggtgg
    22081 gattgtcgcg ataagcgcct tcgcaggtta gcagttcgag agtcggtttt tgtgtttgca
    22141 ttgttattct ctaattttgt tcaaaaaaag cccgctcatt aggcgggctg ggtatctgat
    22201 tgctttacgc atggtgaata cactgcccgt ttcaggaagt gcgccaccaa cctttcagta
    22261 cgaaaattgc tttcattgtc gatacccttt ttacgtgaac ttgcgtacta gttaactagt
    22321 tcgatgatta attgtcaaca gctcatttca gaatatttgc cagaaccgtt atgatgtcgg
    22381 cgcaaaaaac attatccaga acgggagtgc gccttgagcg acacgaatta tgcagtgatt
    22441 tacgacctgc acagccatac cacagcttcc gatggctgcc tgacgccaga agcattggtg
    22501 caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca ccgaccatga caccacagct
    22561 gcaatcgcgc cagcaagaga ggaaatttca cgttcgggac tggcgttgaa tcttattccc
    22621 ggcgtggaaa tttccacggt ctgggaaaat catgaaattc atattgtcgg gctgaatatt
    22681 gatattactc atccgctaat gtgtgagttc ctcgcgcagc agacagaacg gcgcaatcag
    22741 cgggcgcagc tgattgccga aagacttgaa aaagcgcaaa tacctggggc gctggaaggc
    22801 gcacaacgac tggcgcaggg cggcgcagtg acgcgcggtc attttgcgcg tttccttgtt
    22861 gagtgcggca aagccagttc aatggcggat gtctttaaaa agtatctggc gcgcgggaaa
    22921 accggatacg ttccgccaca gtggtgtaca atagaacaag ctattgatgt cattcatcat
    22981 tctggcggta aggcggtgct agctcatcct gggcggtaca atcttagtgc taaatggctg
    23041 aaaagattgg ttgcgcattt tgccgaacac cacggtgacg cgatggaagt cgcgcagtgc
    23101 cagcaatcgc ccaatgaacg tacccagctg gcggcccttg cgcgtcagca tcatttatgg
    23161 gcatcacaag gatctgattt tcatcagcca tgcccgtgga tcgaactggg tcgtaaactc
    23221 tggctgcccg caggcgttga aggcgtctgg cagctatggg aacagccgca gaacaccaca
    23281 gagagggaat tatgagccag tttttttata ttcatcctga taacccacag caacgtctga
    23341 tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat tgtttatcca actgattccg
    23401 gctatgcgct cggctgtaaa attgaagata aaaacgcgat ggagcgtatt tgtcgtattc
    23461 gccagctgcc ggacggtcac aactttaccc tgatgtgtcg cgatctttct gaactctcga
    23521 cctattcatt tgttgataac gttgcgtttc gtttaatgaa aaacaacacg ccgggcaact
    23581 ataccttcat cttgaagggg acgaaggaag tgccacgccg tctgttgcag gaaaaacgca
    23641 aaaccatcgg tatgcgtgtg ccttctaacc cgattgcgca ggcgttgctt gaagcactgg
    23701 gcgaaccgat gctttccact tcgctaatgc tgccaggcag cgaatttacc gaatccgatc
    23761 ccgaagaaat taaagaccgt ctggaaaaac aggtggattt gattatccac ggcggctatc
    23821 tcggacagaa accgacaacg gttattgatc ttaccgatga tacgccggtc gtggtgcgtg
    23881 aaggcgtagg tgatgtgaag cctttcttat aagtgcgtaa ctctgtatac tacgcggcct
    23941 taaaatggcg ctatacggcg ccattgattc tcctgttcga cgcctgtgaa ggcgacacca
    24001 aaaggaagct ctatgagcga aaaattacag aaagtgctgg cccgcgctgg ccacggctcc
    24061 cgccgtgaaa tcgaatctat tattgaagcc ggtcgtgtga gtgttgatgg caaaattgct
    24121 aaactcggcg atcgcgttga agttacccct gggctgaaaa ttcgtatcga tggtcatctg
    24181 atttcggtac gtgagtccgc tgaacaaatt tgtcgcgttc tggcctatta caaaccggaa
    24241 ggtgagttgt gtacccgtaa cgatccggaa gggcgtccga cggtgtttga ccgtctgcca
    24301 aagctgcgtg gcgcacgctg gattgccgta ggtcgtctgg acgttaacac ctgtggtctg
    24361 ctgctgttca ccaccgatgg tgaactggca aaccgtttaa tgcatccaag ccgtgaagtt
    24421 gaacgtgaat acgccgtgcg tgtatttggt caggttgacg acgcgaaact gcgtgatttg
    24481 agtcgtggcg tgcaactgga agatggtccg gcagctttta aaaccatcaa gttcagcggc
    24541 ggcgaaggga tcaaccagtg gtacaacgtg actctgaccg aaggccgtaa ccgtgaagtt
    24601 cgtcgtctgt gggaagcggt tggtgtgcag gtgagccgcc tgatccgtgt tcgttacggt
    24661 gatatcccac tgccaaaagg tctgccacgc ggtggctgga cggagctgga cctcgcccag
    24721 actaactatc tgcgcgaact ggtggagctt ccgccagaaa ccagctctaa agtcgctgta
    24781 gaaaaagacc gtcgtcgcat gaaggctaat cagattcgtc gtgcggtgaa acgtcacagt
    24841 caggtgagcg gcggtcgtcg ttctggcggg cgtaataacg gttaatcaaa gtatgccgga
    24901 cgtcatatcc ggcattttta cagattaata atcaatccct atttgcgcct taacaccggc
    24961 atcaaacgca tgtttgatgg ggcgcagctc acttaccgta tccgccagtt caagaatatc
    25021 ccgatgacag cctcgacccg tgataatcac cgtctgttga tgtggacgtt catttaacgc
    25081 ctgcaccact tcttccagtg gcaaatagtc atacgccacc atatacgtca gttcatcaag
    25141 caaaaccata tccagtgagg aatcagcaag catccgcttt gcatgttgcc agacttcgcg
    25201 gcaggcggcg gtatcagact cgcggttttg tgtatcccag gtaaagcccg ttgccatcac
    25261 ctgaaactca acgccatgtg gctccagcag attgcgttcg ccattaggcc aggtgccttt
    25321 aataaactgc acgacgccta ctttttttcc gtgaccaact gcgcgtgttg ccgtaccaaa
    25381 tgccgcggtg gttttgcctt ttccattgcc ggtaaagacg atgataatac cgcgttcatc
    25441 ctgggcctgg gccacacgag catctacttt ttctttcact cgctgctgac gctgttggta
    25501 gcgttcatca ctcattggga aattcctggt ttacggcccg gttgggcgtc aaaggtcatg
    25561 ccggttttac ggcggctgtc atcgcccatc agccagaggt agagcggcat gatatcagcg
    25621 ggtgttttaa gtttctgtgg atcttcggtc gggaaggcgc tggcacgcat tgcggtgcgc
    25681 gtaccgcctg ggttaatgca gttgacgcgc aggcgctgct gatattcatc ggccagtacc
    25741 tgcatcatcc cttcggtggc aaatttcgac gctgcatatg cgccccagtt ggctcgtccc
    25801 tgacgtccaa cgcttgatga agtaaagacc agtgaaccgg cgtccgattt gagtaataaa
    25861 ggaagcagtg cctgggtgag cataaaggtg gcattaacgt tgacctgtat gacgtcctgc
    25921 cagacctgtg gattttgttc gctcattggg caaacatcgc cgagcaatcc ggcattatgc
    25981 aaaacaccat ccagacgcgg ataattaacg gtaatgcgct gtgccagttg ttggcaatct
    26041 tcggacgtgc aggtcagcaa atcgagaata aaccactgtg gctgacgtcc agttacttcg
    26101 tttatgtggc tggctacctg acgtaatttt tcttcattac ggcccaacag aatcactgtc
    26161 gcaccatagc gtgcatacgt catcgcggct tcacggccaa tcccatcgct ggctcccgtc
    26221 accaggataa tgcgatcatt gagtaaatct tgttttggct ggtaatgcat ggctactcct
    26281 caacgacgtt gtctgtcgtg ctgccagtgt actttatgac tttggggctt tatgcctgaa
    26341 acagaagggc atttcaatca gcctggggcg ataacaccgc aaaatggata ctttgtcata
    26401 ctttcgctgc aataacatct ctgcgagacg gcttaacatg cctgttgtaa actgtgagcc
    26461 aaagcgttgt ttaaccaagg tggggactcg tggaattgtt gtctgaatat ggtttgtttt
    26521 tggcgaaaat cgttaccgtt gtgctagcga ttgcggcgat tgccgccatt attgtcaatg
    26581 ttgctcaacg taataaacgc cagcgtggcg agttacgggt caacaatctc agcgaacagt
    26641 ataaggagat gaaagaagaa ctggccgcgg cgctgatgga ctcacatcag caaaaacagt
    26701 ggcacaaagc gcagaagaaa aagcacaagc aagaagcgaa agcagcaaaa gcgaaagcca
    26761 aactgggcga ggtggcaact gacagtaaac ctcgcgtctg ggtgctggat tttaaaggca
    26821 gcatggacgc ccatgaagtg aactcgctac gtgaagagat aacggcggta ctcgcagcat
    26881 tcaaaccgca ggatcaggtt gtgctccgtc tggaaagccc tggtggcatg gtgcatggtt
    26941 acgggttggc ggcttcgcag ctgcagcgtc tgcgtgataa aaacattcct ttaactgtta
    27001 cggtagacaa agtcgctgcc agcggcggtt acatgatggc ctgtgtggcg gacaaaattg
    27061 tttccgcacc gtttgctatt gtgggttcca ttggggtggt ggcgcaaatg cccaacttta
    27121 accgcttcct gaaaagcaaa gatattgata tcgaactgca cactgccggg cagtataagc
    27181 gtactctgac gttgctgggt gaaaataccg aagaagggcg ggagaaattc cgcgaagagt
    27241 tgaacgaaac gcatcagttg tttaaagatt ttgtgaagcg tatgcgtccg tctctggata
    27301 ttgaacaggt ggcaacgggt gaacactggt acggacaaca ggcggtagag aaaggcctgg
    27361 ttgatgaaat caacaccagt gatgaagtta ttcttagcct gatggaaggc cgtgaagtgg
    27421 ttaatgtacg ctatatgcag cgtaaacgac tcattgaccg attcaccggc agcgcggcag
    27481 agagcgccga tcgattgttg ctacgctggt ggcagcgggg tcaaaagcca ttgatgtaaa
    27541 agacaaacgc gaggctaaga cctcgcgttt tgctttaatc aaccagatga tatttttctg
    27601 aaagcacatg ggccaggtgt ttgaacatat taaacaccgc ggtgcttttg gctgttggca
    27661 atccttgttc atctaaaaag tagtcgccgg taaataccag cacgccatta cgctgcgtga
    27721 cgccggtggc ttcaatccct gcgagcgtat cctcatgctc acgaatgatt ttgttggcct
    27781 ctttcaacag cgtttcgcgg tcgatgggtt gtgtctcttt gttcattatt tactccttaa
    27841 acaaggacat tagtctacgc caggcatggc ttgcagacaa atataccacg ctggtggcaa
    27901 gagcgcctta ctggtaactt tggattttgc atgctaataa agttgcgtat cggattttat
    27961 caggtacagt gtgacgcttt cgtcaatctg gcaatagatt tgcttgacat tcgaccaaaa
    28021 ttccgtcgtg ctatagcgcc tgtaggccaa gacctgttaa ctcagtcacc tgaattttcg
    28081 tgaacagagt cacgacaagg ggttgatatc cgcagagaac gagtccatat cggtaactcg
    28141 ttgccagtgg aaggtttatc aacgtgcgac gcattcctgg aagaatcaaa ttaggtaagg
    28201 tgaatatggg taaagctctt gtcatcgttg agtccccggc aaaagccaaa acgatcaaca
    28261 agtatctggg tagtgactac gtggtgaaat ccagcgtcgg tcacatccgc gatttgccga
    28321 ccagtggctc agctgccaaa aagagtgccg actctacctc caccaagacg gctaaaaagc
    28381 ctaaaaagga tgaacgtggc gctctcgtca accgtatggg ggttgacccg tggcacaatt
    28441 gggaggcgca ctatgaagtg ttgcctggta aagagaaggt cgtctctgaa ctgaaacaac
    28501 tggctgaaaa agccgaccac atctatctcg caaccgacct tgaccgcgaa ggggaagcca
    28561 ttgcatggca cctgcgggaa gtgattggtg gtgatgatgc gcgctatagc cgagtggtgt
    28621 ttaacgaaat tactaaaaac gcgatccgcc aggcatttaa caaaccgggt gagctgaata
    28681 ttgatcgtgt taatgcccag caggcgcgtc gctttatgga ccgcgtggtt gggtatatgg
    28741 tttcgccgct gctatggaaa aagatcgctc gtggtctgtc tgccggtcgt gtgcagtcgg
    28801 tggcggttcg cctggtggtc gagcgtgagc gtgaaattaa agcgttcgtg ccggaagagt
    28861 tctgggaagt cgatgccagc acgaccacgc catctggtga agcgttggcg ttgcaggtga
    28921 ctcatcagaa cgacaaaccg ttccgtccgg tcaacaaaga acaaactcag gctgcggtaa
    28981 gtctgctgga aaaagcgcgc tacagcgtgc tggaacgtga agacaaaccg acaaccagta
    29041 aacctggcgc tccttttatt acctctacgc tgcaacaagc tgccagcacc cgtcttggat
    29101 ttggcgtgaa aaaaaccatg atgatggcgc agcgtttgta tgaagcaggc tatatcactt
    29161 acatgcgtac cgactccact aacctgagtc aggacgcggt aaatatggtt cgcggttata
    29221 tcagcgataa ttttggtaag aaatatctgc cggaaagtcc gaatcagtac gccagcaaag
    29281 aaaactcaca ggaagcgcat gaagcgattc gtccttctga cgtcaatgtg atggcggaat
    29341 cgctgaagga tatggaagca gatgcgcaga aactgtacca gttaatctgg cgtcagttcg
    29401 tagcctgcca gatgacccca gcgaaatatg actccacgac gctgaccgtt ggtgcgggcg
    29461 atttccgcct gaaagcacgc ggtcgtattt tgcgttttga tggctggaca aaagtgatgc
    29521 ctgcgttgcg taaaggcgat gaagatcgca tcttaccggc agttaataaa ggcgatgctc
    29581 tgacgctcgt tgaacttaca ccagcccagc actttaccaa gccgccagcc cgtttcagtg
    29641 aagcatcgct ggttaaagag ctggaaaaac gtggtatcgg tcgtccgtct acctatgcgt
    29701 cgatcatttc gaccattcag gatcgtggct acgtgcgagt agaaaatcgt cgtttctatg
    29761 cggaaaaaat gggcgaaatc gtcaccgatc gccttgaaga aaatttccgc gagttaatga
    29821 actacgactt taccgcgcag atggaaaaca gcctcgacca ggtggcaaat cacgaagcag
    29881 agtggaaagc tgtactggat cacttcttct cggatttcac ccagcagtta gataaagctg
    29941 aaaaagatcc ggaagagggt ggtatgcgcc cgaaccagat ggttctgacc agcattgact
    30001 gcccgacttg tggtcgcaaa atggggattc gcacagcgag caccggggta ttccttggct
    30061 gttctggcta tgcgctgccg ccgaaagagc gttgcaaaac caccattaac ctggtgccgg
    30121 aaaacgaagt gctgaacgtg ctggaaggcg aagatgctga aaccaacgcg ctgcgcgcaa
    30181 aacgtcgttg cccgaaatgc ggcacggcga tggacagcta tctcatcgat ccgaaacgta
    30241 agttgcatgt ctgtggtaat aacccaacct gcgacggtta cgagatcgaa gagggcgaat
    30301 tccgcattaa aggttatgac ggcccgatcg ttgagtgtga aaaatgtggc tctgaaatgc
    30361 acctgaaaat ggggcgtttc ggtaaataca tggcctgcac caacgaagag tgtaaaaaca
    30421 cacgtaagat tttacgtaac ggcgaagtgg caccaccgaa agaagatccg gtgccattac
    30481 ctgagctgcc gtgcgaaaaa tcagatgctt atttcgtgct gcgtgacggt gctgccggtg
    30541 tgttcctggc tgccaacact ttcccgaaat cgcgtgaaac gcgtgcgcca ctggtggaag
    30601 agctttatcg cttccgcgac cgtctgccgg aaaaactgcg ttatctggcc gatgcgccac
    30661 agcaggatcc ggaaggtaat aagaccatgg ttcgctttag ccgtaaaacc aaacagcaat
    30721 atgtctcttc ggaaaaagac ggaaaggcga ctggctggtc agcattttat gttgatggca
    30781 aatgggttga aggaaaaaaa taacctttaa ttctgtcagg tttttataaa caaagggtcg
    30841 cgaaagcggc ccttttttat tgcatattat
//
LOCUS       DAPHJE010000051        29919 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPHJE010000051 DAPHJE010000000
VERSION     DAPHJE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.051"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(31..1485)
                     /gene="xylB"
                     /locus_tag="RH222_002284"
     CDS             complement(31..1485)
                     /gene="xylB"
                     /locus_tag="RH222_002284"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV3797297.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLHLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1557..2879)
                     /gene="xylA"
                     /locus_tag="RH222_002285"
     CDS             complement(1557..2879)
                     /gene="xylA"
                     /locus_tag="RH222_002285"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV3797298.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGVFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3245..4237
                     /gene="xylF"
                     /locus_tag="RH222_002286"
     CDS             3245..4237
                     /gene="xylF"
                     /locus_tag="RH222_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3797299.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            4315..5856
                     /gene="xylG"
                     /locus_tag="RH222_002287"
     CDS             4315..5856
                     /gene="xylG"
                     /locus_tag="RH222_002287"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV3797300.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5834..7015
                     /gene="xylH"
                     /locus_tag="RH222_002288"
     CDS             5834..7015
                     /gene="xylH"
                     /locus_tag="RH222_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV3797301.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7093..8271
                     /gene="xylR"
                     /locus_tag="RH222_002289"
     CDS             7093..8271
                     /gene="xylR"
                     /locus_tag="RH222_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV3797302.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8379..9203)
                     /locus_tag="RH222_002290"
     CDS             complement(8379..9203)
                     /locus_tag="RH222_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV3797303.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9523..11553
                     /gene="malS"
                     /locus_tag="RH222_002291"
     CDS             9523..11553
                     /gene="malS"
                     /locus_tag="RH222_002291"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV3797304.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11731..12984
                     /gene="avtA"
                     /locus_tag="RH222_002292"
     CDS             11731..12984
                     /gene="avtA"
                     /locus_tag="RH222_002292"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV3797305.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13135..13608)
                     /locus_tag="RH222_002293"
     CDS             complement(13135..13608)
                     /locus_tag="RH222_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV3797306.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13710..14558)
                     /gene="yiaJ"
                     /locus_tag="RH222_002294"
     CDS             complement(13710..14558)
                     /gene="yiaJ"
                     /locus_tag="RH222_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDV3797307.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14759..15757
                     /gene="yiaK"
                     /locus_tag="RH222_002295"
     CDS             14759..15757
                     /gene="yiaK"
                     /locus_tag="RH222_002295"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDV3797308.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15769..16236
                     /locus_tag="RH222_002296"
     CDS             15769..16236
                     /locus_tag="RH222_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV3797309.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16354..16827
                     /gene="yiaM"
                     /locus_tag="RH222_002297"
     CDS             16354..16827
                     /gene="yiaM"
                     /locus_tag="RH222_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDV3797310.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16830..18107
                     /gene="yiaN"
                     /locus_tag="RH222_002298"
     CDS             16830..18107
                     /gene="yiaN"
                     /locus_tag="RH222_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDV3797311.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18120..19106
                     /gene="yiaO"
                     /locus_tag="RH222_002299"
     CDS             18120..19106
                     /gene="yiaO"
                     /locus_tag="RH222_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDV3797312.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19110..20606
                     /gene="lyxK"
                     /locus_tag="RH222_002300"
     CDS             19110..20606
                     /gene="lyxK"
                     /locus_tag="RH222_002300"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDV3797313.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            20603..21265
                     /gene="ulaD"
                     /locus_tag="RH222_002301"
     CDS             20603..21265
                     /gene="ulaD"
                     /locus_tag="RH222_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV3797314.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            21258..22118
                     /locus_tag="RH222_002302"
     CDS             21258..22118
                     /locus_tag="RH222_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDV3797315.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22112..22807
                     /gene="araD"
                     /locus_tag="RH222_002303"
     CDS             22112..22807
                     /gene="araD"
                     /locus_tag="RH222_002303"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV3797316.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(22839..22940)
                     /locus_tag="RH222_002304"
                     /pseudo
     CDS             complement(22839..22940)
                     /locus_tag="RH222_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            22944..23132
                     /locus_tag="RH222_002305"
                     /pseudo
     CDS             22944..23132
                     /locus_tag="RH222_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23154..23894)
                     /locus_tag="RH222_002306"
     CDS             complement(23154..23894)
                     /locus_tag="RH222_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV3797317.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            24018..24992
                     /locus_tag="RH222_002307"
     CDS             24018..24992
                     /locus_tag="RH222_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3797318.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(24989..26125)
                     /locus_tag="RH222_002308"
     CDS             complement(24989..26125)
                     /locus_tag="RH222_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3797319.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(26131..26454)
                     /locus_tag="RH222_002309"
     CDS             complement(26131..26454)
                     /locus_tag="RH222_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV3797320.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            26637..26780
                     /locus_tag="RH222_002310"
     CDS             26637..26780
                     /locus_tag="RH222_002310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797321.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(26999..28537)
                     /gene="aldB"
                     /locus_tag="RH222_002311"
     CDS             complement(26999..28537)
                     /gene="aldB"
                     /locus_tag="RH222_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV3797322.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(28702..29853)
                     /gene="yiaY"
                     /locus_tag="RH222_002312"
     CDS             complement(28702..29853)
                     /gene="yiaY"
                     /locus_tag="RH222_002312"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV3797323.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMIADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTDKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         7407 a         7715 c         7480 g         7317 t
ORIGIN      
        1 tcccccaccc ggtcaggcag gggataacgt ttacgccatt aatggcagaa gttgctgata
       61 gaggcgacgg aacgtttcac gtcgtggctg ataagcggca taacgctgcg catctggtag
      121 atgcgactgt tctaacggta gctgcggcaa caactcaatg agcgatttct ctggattcgc
      181 cgcaatctgc gccagccttg ctgcgcccag tgctggcccc acatcccccc ccgtacggta
      241 atcgagctgc tgaccgctga tatccgccag catctgacgc cagtactcac tacgcgcccc
      301 gccaccaatc aacgtaacac tttgcggttt aatgccgcag gcatgcacga catccatgcc
      361 atctgccagc gcataaccca cgccttccag cactgctcgc gccagttcat tggggccatg
      421 ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga ttattgtgtg gtgtacgctc
      481 gccggaaaga taaggcagaa accaaactgg ctcggcactt tcatcagcct gttgagctgc
      541 agcgattaaa gctgggacat tgcacaggcc ggttaatttc gcgacccaat ccagacacga
      601 tgctgcactc agcattacag acattaaatg ccaacgctgc ggtagcgcat ggcaaaagct
      661 gtgtacagcg ctttctggct tgcttaagaa cccttcgctg acagcaaaat agacccccga
      721 cgtccccagc gataacattg cctgattagc atcaaccatt cccacaccaa ctgcaccagc
      781 tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc ataccccacg ctttcgcaac
      841 ttcaggtaac aaagcaccag taatttcgct gccttcgtat aatgcgggca tctggtcacg
      901 agataagtcg caagcctgca gcatgacgtc actccagtca cgctttgcga catccagcca
      961 catggtgcca gctgcgtcag acatatcgct ggcaaactcc cccgtcatac gcagatgcaa
     1021 gtaatctttc ggtaataata ctttgtcgat ttgacggaat atctccggct catgccgctg
     1081 aacccatagc aatttaggcg cagtaaatcc gggcatcatc aggttgccgg taatcactcg
     1141 tgattgcgga actctcgctt ccagcaaagt gcactcttgc gcacagcgcc cgtcgttcca
     1201 caaaatggca gggcgcaata cccgttgttg agcatccagt aaggttgctc catgcatctg
     1261 gccggcaata cccaatgctt taacgtcctg cagagaatgc tgatcgccca gagctttcat
     1321 tgcgcgatca gttgcctgcc accactgttc cgggtcttgt tccgaccaga gtggatgcgg
     1381 gcgcgaaacg gtcagctttt ccgtttgcga agcaaccacc tcaccctgct cgttgagcaa
     1441 aataactttt acgcccgagg tgccaagatc tatcccgata tacatatcga tcgttcctta
     1501 aaaaaatgcc cggtatcgct accgataacc gggccaacgg actgcacagt tagccgttat
     1561 ttgtcgaaca gataatgatt taccagattt tccagttgct cctggcgacc actctgatgc
     1621 accggagaca aattatgttc ctgagcatat ttggctaaat ctgccagtga catttggcct
     1681 ttcaggattt gctggcccaa ttcgctattc cagccggaat aacgctgcgc gatgcgttta
     1741 tccagctcgc catcttcaat catgcgcgct gcaattttca gcgccagtgc catcgtatcc
     1801 atcgcgccga tatgaccgta aaacagatca tatttatcag tactttgacg acgtactttg
     1861 gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt tgagaatttc atacatcacc
     1921 agcgcattct cttccacact gttcgggaac tggtcggtgt cccagcccag ttgcgcatcg
     1981 ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa tggcggtggc tatttcatga
     2041 tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa tgttcagttt aatctctttt
     2101 tccagaccaa actgtttcag gaagccatag accgtcgcgg catcgtaatc atattgatgt
     2161 ttagtcggtt cttgcggttt cggttcgata agcaacgtgc cctggaagcc gattttatgt
     2221 ttatgctcaa ccaccatctg cataaagcgg cccagttgtt cacgctcctg acgcaagtcg
     2281 gtatttaaca gcgtttcgta accttcacga ccaccccaca ggacatagtt ttcaccgccc
     2341 aatttatggg ttgcttccat cgctgtaaca acttgcgttg ccgcccagct gaagacttca
     2401 ggatctgggt tcgtcgccgc acccgcgccg tagcgagggt ttgtaaagca gttggcggtt
     2461 ccccacagca gcttcacgcc gctctcttct tgcttgcctg ccaggacatc aaccatttgc
     2521 gcaaaattat tgatgtactc ttttaacgac gcgccctcag gggaaacatc cacatcgtgg
     2581 aagcaataaa atggcacatg taacttgtgg aaaaactcaa atgcgacatc tgctttacgc
     2641 ttcgccaacg ccagtgcctc accaggctgc tgccacggac gattaaacac ccccacacca
     2701 aacatgtccg ccccgttcca gcagaaggtg tgccagtagc aggcggcaaa acgcaagtgc
     2761 tcttccatac gcttacccaa caccagttcg tcgggattgt agtgacggaa tgctaacggg
     2821 tttgaggatt ttgagccttc ataacgaacg cgatcgagct ggtcaaaata ggcttgcata
     2881 ttgaactcca taatcaggta atgccgcggg tgatggatga tgtcgtaata ttgggcactc
     2941 cctttcagtt gctcaattat gttatttcac actgctattg agataattca caagtgtgcg
     3001 ctcgctcgca aaataaaatg ggatgatgaa actgggtaat tcctcgaaga taaaaatgca
     3061 ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata aatcaagaaa taaaccaaaa
     3121 atcgtaatcg aaagataaaa atctgtaatt gttttcccct gtttagttgc taaaaattgg
     3181 ttacgtttat cgcggtgatt gttacttatt aaagctgtcc tctaactaca gaaggcccta
     3241 caccatgaaa ataaagaaca ttctactcac cctttgcacc tcacttctgc ttaccaacgt
     3301 tgcggcacac gccaaagaag tcaaaatagg tatggcgatt gatgatctcc gtcttgaacg
     3361 ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa tctctcggcg cgaaagtatt
     3421 tgtacagtct gcaaatggca atgaagaaac acaaatgtcg cagattgaaa acatgattaa
     3481 ccggggtgtc gatgttcttg tcattattcc gtataacggc caggtattaa gtaacgttgt
     3541 aaaagaagcc aaacaagaag gtattaaagt attagcttac gaccgtatga ttaacgatgc
     3601 ggatatcgat ttttatattt ctttcgataa cgaaaaagtc ggtgaactgc aggcaaaagc
     3661 cctggtcgat attgttccgc aaggtaatta cttcctgatg ggcggctcgc cggtagataa
     3721 caacgccaag ctgttccgcg ccggacaaat gaaagtgtta aaaccttacg ttgattccgg
     3781 aaaaattaaa gtcgttggtg accaatgggt tgatggctgg ttaccggaaa acgcattgaa
     3841 aattatggaa aacgcgctaa ccgccaataa taacaaaatt gatgctgtag ttgcctcaaa
     3901 cgatgccacc gcaggtgggg caattcaggc attaagcgcg caaggtttat cagggaaagt
     3961 agcaatttcc ggccaggatg cggatctcgc aggtattaaa cgtattgctg ccggtacgca
     4021 aactatgacg gtgtataaac ctattacatt gctggcaaat actgccgcag aaattgccgt
     4081 tgaattgggc aatggtcagg agccaaaagc agatacctca ctgaataatg gcctgaaaga
     4141 tgtcccctcc cgcctcctga caccgatcga tgtgaataaa aacaacatca aagatacggt
     4201 aattaaagac ggattccaca aagagagcga gctgtaagcg ttacgccccg gcgcggagcg
     4261 ggggcgtgat ttctctccat gccgcgtgaa tgaattggct taggtggagt cgttatgcct
     4321 tatctacttg aaatgaagaa cattaccaaa accttcggca gtgtgaaggc gattgataac
     4381 gtcagcttgc ggttgaatgc tggcgaaatc gtctcactct gtggggaaaa tgggtctggt
     4441 aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc atggctccta cgaaggcgaa
     4501 attatttttg cgggagaaga gattcaggcg agtcacatcc gcgataccga acgcaaaggt
     4561 atcgccatca ttcatcagga attggccctg gtgaaagaat tgaccgtgct ggaaaatatc
     4621 ttcctgggta acgaaataac ccacaatggc attatggatt atgacctgat gacgctacgc
     4681 tgtcagaagc tgctcgcaca ggtcagttta tccatttcac ctgatacccg cgttggcgat
     4741 ttagggcttg ggcaacaaca actggttgaa attgccaagg cacttaataa acaggtgcgc
     4801 ttgttaattc tcgatgaacc gacagcctca ttaactgagc aggaaacgtc ggttttactg
     4861 gatattattc gcgatctaca acagcacggt atcgcctgta tttatatttc gcacaaactc
     4921 aacgaagtca aagcgatttc cgatacgatt tgcgttattc gcgacggtca gcacattggt
     4981 acgcgtgatg ctgccggaat gagtgaagac gatattatca ccatgatggt cggacgagag
     5041 ttaaccgcgc tttaccctaa tgaaccacat accaccggag atgaaatatt acgtattgaa
     5101 catctgacgg catggcatcc ggttaatcgt catattaaac gagttaatga tgtctcgttt
     5161 tccctgaaac gtggcgaaat actgggtatt gccggactcg ttggtgccgg acgtaccgag
     5221 accattcagt gcctgtttgg cgtgtggccc ggacaatggg aaggaaaaat ttatattgat
     5281 ggcaaacagg tagatattcg taactgtcag caagccatcg cccaggggat tgcgatggta
     5341 cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg cggttggtaa aaatattacc
     5401 ctcgccgcac tcaataaatt taccggtggc attagccagc ttgatgacgc ggcagagcaa
     5461 aaatgtattc tggaatcaat tcagcaactc aaagttaaaa cgtcgtcccc cgaccttgct
     5521 attggacgtt tgagcggcgg caatcagcaa aaagcgatcc tcgctcgctg tctgttactt
     5581 aacccgcgca tcctcattct tgatgaacct accaggggta tcgatattgg cgcgaaatac
     5641 gagatctaca aattaattaa ccaactcgtc cagcagggta ttgccgttat tgtcatctct
     5701 tccgaattac ctgaagtgct cggccttagc gatcgggtac tggtgatgca tgaagggaaa
     5761 ctaaaagcca acctgataaa tcataacctg actcaggagc aggtgatgga agccgcattg
     5821 aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg aaattggccg taccgacatc
     5881 cggtggtttt tctgggctga agtcactgaa tttgcaggtc ttcgtgatga ttgcagctat
     5941 catcgcaatc atgctgttct ttacctggac caccgatggt gcctacttaa gcgcccgtaa
     6001 cgtctccaac ctgttacgcc agaccgcgat taccggcatc ctcgcggtag gaatggtgtt
     6061 cgtcataatt tctgctgaaa tcgacctttc cgtcggctca atgatggggc tattaggtgg
     6121 cgtcgcggcg atttgtgacg tctggttagg ctggcctttg ccacttacca tcattgtgac
     6181 gttggttctg ggactgcttc tcggtgcctg gaacggatgg tgggtcgcgt accgcaaagt
     6241 cccttcattt attgtcaccc tcgcgggcat gttggcattt cgcggcatac tcattggcat
     6301 caccaacggc acgactgttt cccccaccag cgccgcgatg tcacaaattg ggcaaagcta
     6361 tctacctgcc agcactggct tcatcattgg cgcgcttggc ttaatggctt ttgttggttg
     6421 gcaatggcgc ggaagaatgc gccgtcaggc tttgggtttg cagtctccgg cctctaccgc
     6481 agtagtcggt cgccaggctt taaccgctat catcgtatta ggcgcaatct ggctgttgaa
     6541 tgattaccgt ggcgttccca ctcctgttct gctgctgacg ttgctgttac tcggcggaat
     6601 gtttatggca acgcggacgg catttggacg acgcatttat gccatcggcg gcaatctgga
     6661 agcagcacgt ctctccggga ttaacgttga acgcaccaaa cttgccgtgt tcgcgattaa
     6721 cggattaatg gtagccatcg ccggattaat ccttagttct cgacttggcg ctggttcacc
     6781 ttctgcggga aatatcgccg aactggacgc aattgcagca tgcgtgattg gcggcaccag
     6841 cctggctggc ggagtgggaa gcgttgccgg agcagtaatg ggggcattta tcatggcttc
     6901 actggataac ggcatgagta tgatggatgt accgaccttc tggcagtata tcgttaaagg
     6961 tgcgattctg ttgctggcag tatggatgga ctccgcaacc aaacgccgtt cttgattttg
     7021 ataaaaattt tctcaaagcc ggttacgtat taccggtttt gagtttttgc atgattcagc
     7081 aggaaaagaa ccatgtttac taaacgtcac cgcatcacat tactgttcaa tgccaataaa
     7141 gcctatgacc ggcaggtagt agaaggcgta ggggaatatt tacaggcgtc acaatcggaa
     7201 tgggatattt tcattgaaga agatttccgc gcccgtattg ataaaatcaa ggactggtta
     7261 ggagatggcg tcattgccga cttcgacgac aaacagatcg agcaagcgct ggctgatgtc
     7321 gacgtcccca ttgttggggt tggcggctcg tatcaccttg cagaaagtta cccacccgtt
     7381 cattacattg ccaccgataa ctatgcgctg gttgaaagcg catttttgca tttaaaagag
     7441 aaaggcgtta accgctttgc tttttatggt cttccggaat caagcggcaa acgttgggcc
     7501 actgagcgcg aatatgcatt tcgtcagctt gtcgctgaag aaaagtatcg cggagtggtt
     7561 tatcaggggt tagaaaccgc gccagagaac tggcaacacg cgcaaaatcg gctggcagac
     7621 tggctacaaa cgctgccacc gcaaaccggg attattgccg ttactgacgc ccgagcgcgg
     7681 catattctgc aagtatgtga acatctacat attcccgtac cggaaaaatt atgcgtgatt
     7741 ggcatcgata acgaagaact gacccgctat ctgtcgcgtg tcgccctttc ttcggtcgct
     7801 cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt tgcatcgatt attagataaa
     7861 gaagaaatgc cgctacagcg aattttggtc ccaccagttc gcgtcattga acggcgctca
     7921 acagattatc gctcgctgac cgatcccgcc gttattcagg ccatgcatta cattcgtaat
     7981 cacgcctgta aagggattaa agtggatcag gtactggatg cggtcgggat ctcgcgctcc
     8041 aatcttgaga agcgttttaa agaagaggtg ggtgaaacca tccatgccat gattcatgcc
     8101 gagaagctgg agaaagcgcg cagtctgctg atttcaacca ccttgtcgat caatgagata
     8161 tcgcaaatgt gcggttatcc atcgctgcaa tatttctact ctgtttttaa aaaagcatat
     8221 gacacaacgc caaaagagta tcgcgatgta aatagcgagg tcatgttgta gcgatgatga
     8281 gaattgtcgg cgtcacatca gggaactcag cacagccacc tgatgcgagc aaaagcctta
     8341 tccgaccgac aaatcagata aggctcaagg aaatgcaatt acatatgcgc cgcgattaac
     8401 cgttggttat cctggtacat tgcgaacagg tagttattat aactcttccc tttggtcgag
     8461 tagcccttca gcttgtgaat catcgctgtg gcagtcactt cctgatccgc tttacgcagc
     8521 tgcgcacgcg atttacggaa cgaagagtaa gccgggtgcg tattcaggtt agtgacatag
     8581 gcgctcaccg attctttgac agaactaaac tgtgagtacc ctttcacttt acccggcgca
     8641 ttggtacaac gtcctttcat gcatttcatg ccgaacagat tgttattgtt gcgcgccagc
     8701 ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg ccaccatact ggtagggata
     8761 atgtctacgc gttcaagcaa ggtattccac gggatttttc gcgtattacc agaccacttc
     8821 accttgtagc gtttggcgat gtctttcaga cgcgcacgct cagcaggtga ccattggccc
     8881 tgatactgtt ttgaaatcag ccagttacgt tctgcagtaa tggctgcatt ttggctggta
     8941 atgtaaggca ttacggtccg gagaaacgcc tttttccttg gtgttccgga agggtatttt
     9001 cgcaaatcag gaagtgaact actctttgca ctattgcgag aatactcttg tttactgctt
     9061 acctgtttat tactagcttt agttaagtgg gacttttgac tcgctgttgt tgtgtgcgtc
     9121 ttcgctaaca cctcactcga aaacaccaga gtgagtaaca taagaatcat cgccccatat
     9181 cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga tttaatcatt ccaacacctt
     9241 atatttttca caaatttgag agttgaatct caaatcatat caaaaatagc tgtcaagagc
     9301 accccaagga atagtccaaa tctgaaacta tgtcacgtgt taacgattca gattggcgct
     9361 aaatcgcaga aaatgtgggg tttatcgcaa aattcagccg ttttttgcgc gagatcgctc
     9421 acccttgctt ctcatcctgt ggacttaccg ctcagggatg agttttgtct ggcttatcgc
     9481 tggcaaactg tctgaaatcg cagcaataag gactcatcca ccatgaaact cgccgcctgt
     9541 tttctgacac tccttcctgg cttcgccgtt gccgccagct ggacttctcc ggggttccct
     9601 gcctttagcg aacagggaac gggaacattt gtcagccacg cgcagttgcc caaaggtacg
     9661 cgtccactca cgctaaattt tgaccaacag tgctggcagc ctgcagatgc gataaaactc
     9721 aatcagatgc tttccctgca accttgtagc aacacgccgc ctcaatggcg attgttcaga
     9781 gacggcaaat atacgctgca aatagacacc cgctccggta cgccaacatt gatgatttcc
     9841 atccagaacg ccgccgaacc ggtagcaaac ctggtccgtg aatgcccgaa atgggatgga
     9901 ttaccgctca cgctggatgt cagcgccact ttcccggaag gagccgccgt acgggattat
     9961 tacagccagc aaattgcgat agtgaagaac ggtcaaataa cgttacaacc cgctgctacc
    10021 agcaacggtt tactcctgct ggaacgggca gaaactgacg cccctgcccc tttcgactgg
    10081 cataacgcca cggtttactt tgtgctgaca gatcgtttcg aaaacggcga tcccagtaat
    10141 gaccagagtt acggacgtca taaagacggt atggcggaaa ttggcacttt tcacggcggc
    10201 gatttacgcg gcctgaccaa caaactggat tacctccagc agttgggcgt taatgcttta
    10261 tggataagcg ccccatttga gcaaattcac ggctgggtcg gcggcggtac aaaaggcgat
    10321 ttcccgcatt atgcctacca cggttattac acacaggact ggacgaatct tgatgccaat
    10381 atgggcaacg aagccgatct acggacgctg gttgatagcg cacatcagcg cggtattcgt
    10441 attctctttg atgtcgtgat gaaccacacc ggctatgcca cgctggcgga tatgcaggag
    10501 tatcagtttg gcgcgttata tctttctggt gacgaggtga aaaaaacgct gggtgaacgc
    10561 tggagcgact ggaaacctgc cgccgggcaa acctggcata gctttaacga ttacattaat
    10621 ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa actggatcag aacggatatc
    10681 ggcgattacg acaatcctgg attcgacgat ctcactatgt cgctggcctt tttgccggat
    10741 atcaaaaccg aatcaactac cgcttctggt ctgccggtgt tctataaaaa caaaacggat
    10801 actcacgcta aagtcatcga aggctttaca cctcgcgatt acttaaccca ctggttaagt
    10861 cagtgggtcc gcgactatgg gattgatggt tttcgggtcg ataccgccaa acatgttgag
    10921 ttgcccgcct ggcagcaact gaaaaccgaa gccagcgccg cgcttcgcga atggaaaaaa
    10981 gctaaccccg acaaagcatt agatgacaaa cctttctgga tgaccggtga agcctggggc
    11041 cacggcgtga tgcaaagtga ctactatcgc cacggcttcg atgcgatgat caatttcgat
    11101 tatcaggagc aggcggcgaa agcagtcgat tgtctggcgc agatggatac gacctggcag
    11161 caaatggcgg agaaattgca gggtttcaac gtgttgagct acctctcgtc gcatgatacc
    11221 cgtctgttcc gtgaaggggg cgacaaagca gcagagttat tactattagc gccaggcgcg
    11281 gtacaaatct tttatggcga tgaatcctcg cgtccgttcg gtcctacagg ttctgatccg
    11341 ctgcaaggta cacgttcgga tatgaactgg caggatgtta gcggtaaatc tgccgccaac
    11401 gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc atcccgcaat tggcgcgggc
    11461 aaacaaacga cactttcgct gaagcagggc tacggctttg ttcgtgagca tggcgacgat
    11521 aaagtgctgg tcatctgggc tgggcaacag tgacttttcc ggcttcccgt tcgtcagtac
    11581 ctcgggaagc cgccaaccag gataaaatgt cagccctaat cagcgttgca ggataaagca
    11641 ccgctcactc tccaacagac cgatttgcac cccagcaaat gtagcgttat tgttaccttc
    11701 cttgctacag agttcgacag atatcccgct atgacattct ccctttttgg tgacaaattt
    11761 acccgccact ccggcattac gctgctgatg gaagatctga acgacggttt acgcacgcct
    11821 ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca ggactacttc
    11881 cagacgctac tgaccgacat gctggaaagt ggcaaagcga ctgatgcact gtgcaattac
    11941 gacggtccac aggggaaaac ggagctactc acactgcttg ccggaatgct gcgcgagaag
    12001 ttgggttggg atatcgaagc acagaatatt gcactaacaa acggcagcca gagcgcgttt
    12061 ttctacttat ttaatctgtt tgccggacgc cgtgccgatg gtcgggtcaa aaaagtgctg
    12121 ttcccgctgg caccggaata cattggctac gctgacgccg gactggaaga agatctgttt
    12181 gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc agtttaaata ccacgtcgat
    12241 tttgagcatc tgcatattgg cgaagaaacc gggatgattt gcgtctcccg gccgacgaat
    12301 ccaacaggca atgtgattac tgacgaagag ttgctgaagc ttgacgcgct ggcgaatcaa
    12361 cacggcattc cgctggtgat tgataacgct tatggcgtcc cgttcccggg tatcatcttc
    12421 agtgaagcgc gcccgctatg gaatccgaat atcgtgctgt gcatgagtct ttccaagctg
    12481 gggctgcctg gctcccgctg cggcattatc atcgccaatg aaaaaatcat cactgccatc
    12541 accaatatga acggcattat cagcctggca cctggcggta ttggtccggc gatgatgtgt
    12601 gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa accgttttac
    12661 taccagcgtg ttcaggaaac tatcgccatc attcgccgct atttaccgga agatcgctgc
    12721 ctaattcata aaccagaagg agccattttc ctctggctat ggtttaagga tttgcccatt
    12781 acgaccgagc aactctacca gcgcctgaaa gcacgcggcg tgctgatggt gccggggcac
    12841 aacttcttcc cgggactgga taaaccgtgg ccgcatacgc atcaatgtat gcgcatgaac
    12901 tacgtaccag agccggagaa aattgaggcg ggggtgaaga ttctggcgga agagatagaa
    12961 agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc tacgcttatc aggcctacat
    13021 aatttctgca atatattgaa tttggaagaa tttgtaggcc ggataaggcg tttacgccgc
    13081 atctggcata aacaaagcgc actattccgg cgttgagaaa cgccggaaaa cgtttcaaac
    13141 aggctgctgc cgtagccgat gccgctgcac tttctcgaca tccatgcact gcaacgcctt
    13201 tgtcgggcag gcttcaacac aggccggccc cgtctcccga tgccagcaca gatcgcactt
    13261 aatcgccctc gcctttttgc gcgaagaaac gacctccatc gcaccaaacg ggcaagccag
    13321 catacagctt ttacagccaa tgcaacgtgt ttgttcaacg aaaatatgcc catgttcgcg
    13381 gcttatcgcg ttaacagggc agacattcgc gcacggtgca tcttcacact gatgacaggc
    13441 tactgccgtg gtccagctgt ggtctttaat gacacgaata cgggaaataa actcgtctgg
    13501 tgacaacgca gcgcaatcct gattctcctg atgcgacact gcgcaagcca cttcacaggt
    13561 acggcaaccg atacatttcg tcgcatccgc aataataaac cggttcatga tcactccttt
    13621 gcactaaaac agcagagtgc cagagagcgc gtccctactg ctttgatcca acgaaggaaa
    13681 tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg cgccctggtc gtccctgaca
    13741 gtaaatccca gttcattaga aatagcctgc gcggtttcac gcagtggttt caggagattt
    13801 ttctccccca cctgtttcag acgtgatgtc gaaagcgaaa tcgacacggc gtacggcacc
    13861 cgcccatgaa tattaaacac cggaacagca atacaggaga cgccgagttc gttttcttct
    13921 ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt cgtcgaacat cgcgggcagc
    13981 tcggtaatgg tattgcgggt taacggctgg atctcatgct gatggttttc ccagtatgac
    14041 ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc ccattgcgga acagtagagc
    14101 ggcatatgct ggccaatata ggcacgggtt cgcagcatcc cggttgtggg ttccagctta
    14161 taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt taatggtttc accagtggcg
    14221 atgttcagtg cctcaagatg cggagcggcg atatgaatga tattcagcga agacagcgcc
    14281 ttctgcccga cggcaataaa tttggtagtc aggcggtaac tccctgcggc gggcgcggtg
    14341 gtcacatagc cgcaggactg taatccctgc aataagcgat ggacggtact cttatttaaa
    14401 ccagccagct ccgaaagatg cgccaacgga caaccgtttg gatagttgct caaaatctca
    14461 atcagcatca acccgcgaaa cagactctgg cttccggctg gacgctcttt ttcctgcgcc
    14521 atctcgttct ctttttttcc catcacttct ttccccattt tgtcgcgtcc tgatggtagc
    14581 gcaaagtgtg ccgtagttca cgatctcgac agataattta taaccacttg atttttatgt
    14641 cttttgaaat tcatcaatca gattgctttg ttaaaaagtg atcgatatat ttgaaatcaa
    14701 gtttcgcata ttgaaatttt aagccaaaaa agcgatcaaa aaaacaagga agcctgggat
    14761 gaaagtgaca tttgagcagt taaaagcagc ctttaatcgg gtcttaattt cacgcggcgt
    14821 tgacagcgaa acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg aatccggcgt
    14881 ttattctcac ggcgttaatc gtttccctcg tttcattcaa caactggaaa acggcgatat
    14941 cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac agtgggatgc
    15001 ccagcgttcg atcggcaacc tgacggcgaa aaagatgatg gatcgcgcca ttgaactggc
    15061 tgccgatcac ggtattggtc tggtggcact tcgtaatgcc aaccactgga tgcgcggcgg
    15121 cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga ccaactccat
    15181 cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatt ggcactaacc cgctgatcgt
    15241 cgccattcct tccacgccga tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg
    15301 catgttagaa gttaaccgcc tggcgggccg tcagctcccg gtcgatggtg gctttgatga
    15361 tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat
    15421 gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta ctctcctttc
    15481 cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcatttcaca
    15541 aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg atgccaagct
    15601 gcaacgtatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc aggccatccg
    15661 cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg gtattaccgt
    15721 tgatgacagc gtgtgggcca aaatccaggc gttataagga gataagtcat gatttttgga
    15781 catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc gctcgatttt
    15841 ctgcgcgcta ccgatttcaa cgccctggag ccgggcgttg tcgaaatcga cggcaaaaat
    15901 atttatgcgc aaattatcga tttaaccaca cgcgaagcgg tggaaaaccg tccggaagtg
    15961 catcgtcggt atatcgatat tcagtttctg gcgtggggtg aagagaaaat cggcattgct
    16021 attgatacgg gaaataataa agtcagcgaa tcactattag agcagcgcga tattattttt
    16081 tatcatgaca gtgaacatga atcatttata gaaatgatac cgggcagcta cgcaattttc
    16141 tttccgcagg atgttcaccg accaggttgt attttgcaaa cagcctctga gatacgaaaa
    16201 attgtggtta aagtcgcatt aacggcgctg aattaatcga ataatcgtct acgccagaac
    16261 gactgggtaa tgttattgct ctaactatat taataattta ggttgctgca catgcaactt
    16321 gaattatgac agacatagcc tcaaggaata gctatgaaaa aaatacttga agcaatactg
    16381 gcgattaatc tcgccgtact ttcctgtatt gtgtttataa acattatttt aagatatggt
    16441 tttcagacaa gcattttatc tgtagatgaa ttgtcacgtt atttatttgt ctggttaaca
    16501 tttattggcg cgattgtggc ttttatggat aacgcccacg ttcaggtcac ctttctggtg
    16561 gagaaactct cccccgcatg gcaacgacga gtcgctctgg ttacccattc cttaatctta
    16621 tttatttgtg gcgcactggc ctggggcgca acgctaaaaa caattcagga ctggagcgat
    16681 tattcaccga tcctcggttt acccatcggc ctgatgtatg ccgcttgtct gcccaccagc
    16741 ctcgttatcg cattctttga actgcgtcat ttatatcaac tcatcacgcg tagcaattct
    16801 ctaacgtcgc caccgcaagg agcttaatca tggctgtgct gatttttctg ggctgtctgt
    16861 tgggtggtat tgctatcggt ttgcctattg cctgggcact gttgttgtgc ggggcggcgt
    16921 taatgttctg gctggacatg tttgatgtcc agatcatggc acaaacgctg gtgaacggag
    16981 ccgatagctt ctccctgctg gcgattccgt tctttgtgct ggcgggtgaa atcatgaatg
    17041 cgggcggctt gtcaaaacgc attgttgacc tgccgatgaa actggtgggg cataaaccag
    17101 gcgggctggg ctacgtcggc gtgctggcag ctatgattat ggcgagcctt tctggttctg
    17161 ccgttgcgga taccgccgcc gttgccgctc tactggtgcc gatgatgcgc agcgccaact
    17221 acccggtcaa ccgggcggcg gggctgattg cctctggcgg cattatcgcg ccaattattc
    17281 ccccttccat tccgtttatt atcttcggcg tttccagcgg attatccatc agtaagctgt
    17341 ttatggcggg cattgcccct ggcatgatga tgggcgcaac gctgatgctt acctggtggt
    17401 ggcaggccag tcgccttaat ttaccccgcc agcaaaaagc aacgatgcag gaaatctggc
    17461 actcttttgt ctccggtatc tgggcgctgt ttcttccagt catcattatt ggcgggtttc
    17521 gctccggtct gtttacccct accgaagccg gggccgttgc tgctttctat gcgctgtttg
    17581 tcgccacagt tatttaccgt gaaatgacct tcgccacact ttggcatgta ctcattggcg
    17641 cggcgaaaac cacctcagtg gttatgtttc tggtggcctc ggcacaagtt tccgcctggc
    17701 tgattaccat tgctgaactg ccgatgatgg tttctgattt actgcaaccg ttggtcgatt
    17761 caccgcgtct gctgtttatc gtcattatgg tggctatcct gattgtcggc atggtcatgg
    17821 atttaacgcc gacggtatta attcttaccc cggtattaat gcctttagtt aaagaggcag
    17881 gaatcgatcc gatttatttc ggtgtcatgt ttatcattaa ctgctcaatc ggcttaatta
    17941 caccgcctat cggtaatgta cttaacgtta tttcaggggt ggcaaaactc aaattcgatg
    18001 atgcagtcag aggcgttttc ccttacgtcc tggttttata ttcattatta gtcgtatttg
    18061 tttttattcc cgatctcatc atcctccctt tgaaatggat taattaaaag gaaaatatta
    18121 tgaaattacg ctctgtaacc tacgcattat tcattgctgg cctggctgca ttcagcacat
    18181 cttctctggc ggcgcaatct ttacgtttcg gttatgaaac ctcacaaacc gactcgcaac
    18241 atattgcggc gaaaaaattc aatgatttat tgcaggagag aaccaaaggc gagctgaaat
    18301 taaaattgtt tccggatagc accctcggta acgcgcaggc gatgatcagc ggcgtacgtg
    18361 gcggcaccat cgatatggaa atgtccggct cgaataactt tgccgggtta tcaccagtga
    18421 tgaacttgct tgatgtccct ttcctgttcc gcgataccgc tcacgcgcat aaaacgctcg
    18481 acggcaaagt cggtgatgat ctgaaagcct cacttgaagg taaaggacta aaagtactgg
    18541 cctactggga aaacggctgg cgcgatgtca ccaactcgcg cgcaccggtt aaaacccccg
    18601 ccgacctgaa agggctgaaa attcgcacca acaatagccc gatgaatatc gccgcattca
    18661 aagtctttgg cgctaacccg atcccgatgc cgtttgccga agtctatacc gggctggaaa
    18721 cccgcactat cgacgctcag gaacacccga tcaacgtcgt ctggtcagca aaatttttcg
    18781 aagtgcagaa gtacctttct ctgacgcacc acgcctattc cccgcttctg gtggtgatca
    18841 acaaagcgaa gtttgatggc ttaaccccgg agttccagca agcgctaatt tcatctgcgc
    18901 aggaagcggg taactaccag cgcaaactgg ttgccgaaga tcagcaaaaa atcatcgacg
    18961 gcatgaaaga agcgggcgtg gaagtcatca ccgatctcga ccgcaaagcc tttagcgacg
    19021 cactgggtac tcaggtccgc gacatgtttg taaaagatgt tccgcagggt gctgatctac
    19081 tgaaagccgt ggatgaggtg caataaacca tgacgcaata ctggctgggg ttagattgtg
    19141 gcggtagctg gctgaaagcc gggctgtatg accgcgaagg ccgggaggca ggcgtgcagc
    19201 gcctgccgct gtgcgcatta agcccgcagc caggttgggc agagcgcgat atggcagaac
    19261 tgtggcaatg ctgcatggct gtcattcgcg aactgcttac ccattccggc gttagcgggg
    19321 aacaaattgt cggtatcggt atctccgcac aaggaaaagg cttgtttttg ctggataaaa
    19381 acaacaagcc gctcggtaac gccattttgt cctcggatcg ccgggcgatg gaaatcgttc
    19441 gtcgctggca ggaagatggc atcccggaaa aactctaccc gttgacccga caaaccttgt
    19501 ggaccgggca tccggtgtcg ctgttacgct ggctgaaaga gcacgaaccg gaacgctacg
    19561 cgcaaattgg ctgcgtgatg atgacgcacg actacctgcg ctggtgttta accggcgtca
    19621 aaggctgtga agagagcaat atttccgagt ccaacctcta caacatgagt cttggggaat
    19681 atgacccgtg cctcaccgac tggctgggga ttgctgaaat caatcacgcc ctgccgcctg
    19741 ttgtcggatc tgccgaaatc tgcggggaga tcaccgctca gacagccgta ctgaccggtc
    19801 tgaaagcggg tacgcccgtt gtcggcggcc tgtttgatgt ggtttccacc gcactctgcg
    19861 ccgggatcga agacgaattt acccttaatg cggtgatggg cacctgggcg gtgaccagcg
    19921 gcataacccg cggtttacgt gacggtgaag cgcatccgta tgtctatggt cgctacgtta
    19981 acgatggtga atttatcgtt cacgaagcca gccccacctc ttccggcaac ctcgaatggt
    20041 ttaccgcaca gtggggagaa atctcgtttg atgagatcaa ccaggccgtt gccagcttgc
    20101 cgaaggccgg tggcgatctc tttttcctgc cgttcctgta cggcagcaac gccggactcg
    20161 agatgaccag cggtttctac gggatgcagg ccattcacac ccgcgcacac ctgttgcagg
    20221 ccatttatga aggggtggtg ttcagccata tgacccatct caaccggatg cgcgaacgtt
    20281 ttactgatgt gcacaccctg cgcgtcactg gcggcccggc gcattccgat gtctggatgc
    20341 aaatgctggc ggacgtcagc ggtctgcgta ttgagctgcc gcaggtggaa gaaaccggct
    20401 gctttggtgc agcacttgcc gcccgcgtcg gcaccggggt ttatcgcgat ttcagcgaag
    20461 cccagcgtga tttacagcac ccggtgcgca ccctgctgcc ggatatgact gtccatcagc
    20521 tttaccaaca aaaataccaa cgctatcagc atctcattgc cgcacttcag ggctttcacg
    20581 cccgcatcaa ggagcacata ttatgagccg accacttctg caactggcac tcgaccactc
    20641 atcacttgaa gccgcacagc gcgacgtgac gcagttaaaa gacagcgtcg atattgtcga
    20701 agcgggcacc attctctgtt taaacgaagg gcttggcgcg gtgaaagcct tgcgcgaaca
    20761 gtgcccggac aaaatcatcg ttgctgactg gaaggtcgcc gacgctggtg aaacgctcgc
    20821 gcaacaggcg tttggcgcag gcgctaactg gatgaccatc atctgcgccg cgccgctcgc
    20881 gacggtagaa aaaggccacg caatggcaca acgctgcggg ggtgaaattc agatagagct
    20941 gttcggtaac tggacgctgg acgacgcccg cgactggcat cgtattggcg tgcggcaggc
    21001 catttatcat cgcggtcgtg atgcacaggc cagcgggcaa cagtggggcg aagccgatct
    21061 ggcacgcatg aaggcgcttt cagatatcgg ccttgagctt tccattactg gcgggattac
    21121 ccctgctgac ctgccgctgt ttaaagatat ccgcgtgaaa gcgtttattg ccgggcgggc
    21181 actggcaggc gcggcaaatc cggcgcaagt ggctggcgat ttccatgcgc aaatcgacgc
    21241 tatctgggga ggtgcgcgtg cgtaatcatc cgttagggat ttatgaaaaa gcgctggcga
    21301 aagatctctc ctggccggag cggctggtgc tggcaaaaag ctgcggtttt gattttgtcg
    21361 aaatgtcggt ggatgaaacc gacgaacggc tctcacgtct tgactggagc gccgcacaaa
    21421 ggacttcgct ggttgccgcg atgatcgaaa caggtgttgg gatcccgtcg atgtgcctgt
    21481 ccgcacatcg tcgctttccc tttggtagcc gtgacgaggc agtgcgcgaa cgggcgcggg
    21541 aaatcatgag taaagcgatt cgcctggcgc gcgatctcgg cattcgcacc atccagctgg
    21601 caggttatga cgtctattac gaagaccacg acgaaggcac ccggcaacgt tttgctgaag
    21661 ggctggcgtg ggcagtcgaa caggcggcgg catcacaagt aatgctggcg gtggagatta
    21721 tggataccgc gtttatgaac tccatcagca aatggaaaaa atgggacgag atgctcgcct
    21781 cgccgtggtt caccgtctac ccggacgtcg gcaacctcag cgcctggggc aatgatgttc
    21841 ccgccgaact gaaactgggc attgaccgta tcgccgcgat ccacctgaaa gacacccagc
    21901 cagtcaccgg gcaaagcccc ggacagttcc gtgatgtgcc gtttggcgaa ggctgcgtcg
    21961 atttcgttgg catcttcaaa acgctgcatg aactgaacta tcgcggttct ttcctgattg
    22021 agatgtggac cgaaaaagcc aaagagccgg tgctggagat cattcaggcg cggcgctgga
    22081 ttgaagcgcg tatgcaggag gctggattta tatgttagag caactgaaag ccgacgtgct
    22141 ggcggcgaat ctggcgcttc ccgctcacca tctggtgacg ttcacctggg gcaatgtcag
    22201 cgcggtagac gacacgcggc aatggatggt aatcaaacct tccggcgtcg agtacgacgt
    22261 gatgaccgcc gacgatatgg tggtggttga gatagccagc ggcaaggtgg tggaaggtag
    22321 caaaaagccc tcgtccgata cgccaacgca tctggcactt taccgtcgct atgccgaaat
    22381 tggtggcatt gtgcataccc actcgcgcca cgccaccatc tggtcacagg ccgggctgga
    22441 tctccccgcc tggggcacca cccacgccga ttatttttac ggtgctatcc cctgcacgcg
    22501 acagatgacc gcagaggaga ttaacggcga atatgaatat cagaccggcg aagtgatcat
    22561 tgaaaccttc gaagaacgtg gcaggagtcc ggcacaaatc ccggcggtgc tggtgcattc
    22621 tcacggcccg ttcgcatggg gtaaaaacgc cgccgatgcc gtgcataacg ccgtagtact
    22681 cgaagaatgc gcctatatgg gtctattctc gcgccagctt gcgccgcagc tccctgcgat
    22741 gcaaaacgaa ctgctggata agcactacct gcgtaagcat ggggccaatg cctattacgg
    22801 gcagtaatcc ctcacgccgg ggcttcatcg ccccggcact acgaattgat atgttccttg
    22861 ctgtaacgcc gcttccacgc tgctggcgtt aaaccagtat gtttctgaaa aatctgccgg
    22921 aaatagccaa cgtcattaaa ccgtattgca tggagcaggc cgacaacgtc gagtcactgc
    22981 ataacctgtt gctgcccgcc gatgcgccat ttacgacatt tgaaggcaag ggattgttca
    23041 gccataagat cctgattcag acgccaggtt cccggtatga acagagcaat ccagagcaac
    23101 aacctttgtg gcattacgac ggagcaccag ccgcaccgac atccaccggt gaattaaaaa
    23161 cgccagctca ccccggtgac aaaggtaaaa ctctcattac gatcaatcat cgggctgtcg
    23221 gtaatctctt ccggcagctc gctgtatcct gcgctcgcca tcaatacgac gtgctctcct
    23281 atcgggtatt ttgccgtcag gctgacatag ggcacccagg catcctgcgc ggaataactt
    23341 gccagaccgc tacgacggga ctcactctct gaaatgccat agtaatagtc actgaaattc
    23401 tcgtcataat agagaacgcc cagcgcaggt gtcagcgaca ggcgacctat ctgcattttg
    23461 tggaataccg atagctcccc cacccagccg ttgctgttat ccagaacgtc cgcagccgca
    23521 gaggctttca cggttcccca acgctcgtgg tgataccagg caacccccgc catagccgta
    23581 ctatcacgct tatcaagctg ttgcatggca taatcgtcat tatcggcagg gtcaaattcc
    23641 agcggcatcc aggatgcggt caggctaagt tcattttttt cactttgcga cagaataaaa
    23701 cctaacgtgg tctgacgaac ataaaaagaa tcaccttcat aactaatcag cggaattgcc
    23761 tttgtatttt tattataacc gcgataaggc gattcattat aagccgcacc agcaccaatg
    23821 gataattcag aagcagttgc gcttgccata aaaggcaacg caaataacgc cacaatattg
    23881 cggttaatta acatgtccgg tattccattt taaaataaga taaaaaagag tcggcatagt
    23941 aatgcttaca atttcttcgg tgcaagattc atatttatat agtaatatat aaaattatat
    24001 ataattgggc tgttgcgatg acaaaattac aacttaagta ccgggagtta aaaattatct
    24061 cggtaatcgc tgccagtgaa aatatcagcc atgccgcgac tgtacttggc atcgcacagg
    24121 ccaacgtcag caaatacctt gctgattttg aatcaaaagt gggtttaaaa gtctttgacc
    24181 gaaccacccg gcagctgatg ctcacacctt ttggtaccgc gctgttaccg tacatcaacg
    24241 acatgctgga cagaaacgag cagcttaata attttattgc agattataag catgagaaac
    24301 gtggtcgggt caccatctac gccccaaccg gcataattac ctatttatcc aaacatgtaa
    24361 tcgataaaat taaagatatc ggtgatatca ccttatcgct aaaaacctgc aatcttgagc
    24421 gtaatgcctt ttatgaaggc gtcgaatttc ctgatgattg tgatgtttta atcagctacg
    24481 ctccaccaaa agatgaatcg ctggttgcca gttttattac ccaatatgcc gtaaccgctt
    24541 atgccagcca gcgctatctt gagaaacatc ctattagccg tcctgacgaa ctcgaacatc
    24601 actcctgtat tttgatcgac tcgatgatga ttgatgacgc gaatatctgg cgcttcaacg
    24661 tcgccgggag taaagaggtg cgggattatc gcgtgaaggg aaattacgtt tgcgataaca
    24721 cgcaatcggc gctggagctg gcacgaaatc acctggggat tgtgtttgcg ccagacaaaa
    24781 gtgtgcagag cgatctgcaa gacggcacgc tggtgccctg ctttcagcac ccgtatgaat
    24841 ggtggctgga tctggtggct atcttccgta aacgggaata ccagccctgg cgggtccagt
    24901 atgtgctgga tgaaatgctg cgtgaaattc gccaccagct tgctcagtcg cagcaactgc
    24961 ggcctgaaca agcggcggaa agcgaagatt aatgatcgag ataaaggtaa tgcacccaac
    25021 tggtcatgcg taacagcact ttgcgcatca ccgagacatg gctgaaatga tcagagtaga
    25081 ccgccacctg ggcgtaaata ccgtctggta aagcgctcaa atcagtgtgc tcatccagtt
    25141 caatggtcgc gataacgcca tctgaacccg gcgctgtgtt taacgtctgt aaggtgccgg
    25201 tcgactgata agctccgccg ggaacagctg gactaatggc tgccagctta ccgctgaaca
    25261 ctttgcctgg cagagcgtta aataccactt ccgcatcatc gccgggagcc aggcgcagca
    25321 aggagttctg acggaactgc gccacgattt gtcgtttctg atcgggtata aacaccatca
    25381 ccggacgtag cggcagcgac gcggcatagg tacccggacg aatcagcacc tgggtcacat
    25441 aaccatcgct cggcgcacgc actatcgtct gctcaaggtt atattttgct tccgcgagct
    25501 gcgctttcag gctggcgatt tgagaatgtt cacccaacac caggctatcc agctggctct
    25561 ggatctgttt ttgttccgcc gccgatgact ttacggaggc ttcctgcgcc agataatttt
    25621 gccgcgccac atcaatatcg cgttctgaaa acgggtttac tttcgcctgg ctgccgcgtg
    25681 cgtaacgctg atactcttta gcgaatttat cccgcgtggc ctttgcctgc tgagtattcg
    25741 ccgccatctc atctaactct gcgcccaacg cccgctgttt atgttctgcg gtaacgatat
    25801 ccgccatcag ccgatccacc cgcgcctgat aacgcgtcgg gtccagtcga aatagcacct
    25861 cacctttttt aatcagcgta tttttcttat ccgtcacttc gatcaccacg cctgtcacct
    25921 gtgggacaac aggaatagaa ataaccgctt tttgcgcttt gaaggtatac ggatggttat
    25981 agttcattaa cagaattaga ccgctgacaa taaatattcc acccagggcc gctgtgggaa
    26041 tggtccattt atttacggga attttgaaga tcttaaacat tgcccatgca aaagccacat
    26101 aggtcaaaat aatcagtaaa tccataagta ttactcggca gaagaggatt tgatgtcggc
    26161 gagctgtttc tccagcccgg caatgcgctg ttgcagttgc ataacggatg aatcatggct
    26221 ttgcattccc catccccgct ccgggcggta aagcgtggcc caaatccaca aaaacggcca
    26281 gataacatgt aacgtaaaaa gactcaccca accagcaaca tgaatggcgt cggcatgagg
    26341 atgattacgt tttttggcaa tcaggtaggg aatatcatgt aaaataatga tcccatagaa
    26401 gatcaccaga aatacaaaaa taagcactcc cagtgcaaaa tagtccagga acatatttcc
    26461 ctcaaagaat ataaaaaaga acaattaacg catattatga aatgccatga tgcagtgcaa
    26521 tttccttatt tatataacga catattgttt tttatatact ttcttaaatg tgctgtctgg
    26581 tttttcaaca ggacgaggtt tatcatattg aaatagtgga actattggcc aaactaatga
    26641 ataactccag taaaacatcc acagtacaga ttaagcgtat taaaccttca attatctacc
    26701 gtttattgct gattggcctc ggatcaccaa tggtgattta cggcctggtt cgcccgctca
    26761 ccatcgaaac gcgagattaa actcctgaca aaacacgccc cagaaacgca cttctgttgg
    26821 cgtagtctgg gttattgcgc acgtaggttt aaaacgtaaa ttgtaacaac gtgcgctttg
    26881 tttatgccgg atgcggcgtg tacgccttat ccggcctacg gtcaggttcc cgtaggcatg
    26941 ataagacgcg taagcgtcgc atcaggcaat gaatacccaa tgcgaccagc ttcttatatc
    27001 agaacagccc caacggttta tccgagtagc tcaccagcag gcacttggtt tgctggtaat
    27061 gctccagcat catcttgtgg gtttcgcgac cgatacctga ttgtttgtag ccaccaaacg
    27121 cagcatgtgc cgggtaagcg tgatagcagt tggtccacac gcgcccagcc tgtatgccgc
    27181 gccccatctt ataggccaga ttaccgttgc ggctccagac gcccgcgccc aggccatatt
    27241 gcgtatcgtt cgccagctcc agcgcttctt ccatcgtttt gaaggtggtc accgccagca
    27301 ccgggccaaa aatctcctcc tggaacaccc gcatattgtt ctgaccaaac agaatcgtcg
    27361 gttcgaggta gtagccgtct ttcagttcac cttccagcag cttgcgccgc ccgcctgtga
    27421 gcacgtcagc gccctctttt ttaccgatat caatgtagtt gaggatggtt tccagttgcc
    27481 cgtgagaaac ctgcgcgccc atttgcgtca cgctgtcgag cgggttaccg ctgcgaatgc
    27541 tttcgacacg gcggatggcg cgttccataa agcgttcgta gatagattcc tgcactaaag
    27601 cacgactcgg acaggtgcaa acttcgccct ggttaaaggc aaacagtgca aagccttcca
    27661 gcgctttatc gaaaaaggca tcttcttcat ccatcacatc agcaaagaag atatttggcg
    27721 acttaccgcc caactccagc gtcaccggaa taatgttttg cgttgcgtat tgcataattt
    27781 gttggcccac ttccgttgag ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca
    27841 gatattcgcc aatttcccca cctgcgccat tgaccacgtt caccacaccc ggcggcagta
    27901 aatcaccgac aatttccatt agcagcagta cagaaagcgg ggtaagacgt gcgggtttca
    27961 gcaccacaca gttgcccgcc gccagcgcgg gagccatttt ccagctcgcc atcagcagcg
    28021 ggaagttcca cgggataatc tgccccacca cgcctaacgg ttcatggaaa tgataggcca
    28081 cggtttcgct atcaacttca ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat
    28141 agcggaaatg gtcaatcgcc agcggtacat ccgcagcact ggtttcgcga atgggtttgc
    28201 cgttatccca ggtttcagct gtcgctaaca gctcgaggtt ttgttccatt cgatcggcaa
    28261 tcttaaacag aatcgccgcg cgatcctgca ccgaggtgtg cgcccattta tctttcactt
    28321 tgtgcgcagc atccagcgcc agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca
    28381 gcagctgccc ggtcaccggc gtcagattct ggtaatactc gccgtcggca ggggctaccc
    28441 attcgccgcc aataaagttg tcatagcggg cttttaactt gagggggaaa ccatactcgc
    28501 cgggcttaat ctgtgctgaa gggggattat tggtcatgat cgtctccttg cggtgtgagg
    28561 tataacaagg gtagacgtga ctggcgaaat cttcgccagt cggtaacagc tttacgacag
    28621 ctatcacgaa tttacgggca agggttacag agatagttga aaaagcggct gacaatttgc
    28681 cagccgttgt ggaaatgatg attacatcgc tgcgcgataa atcgccacaa tttcttcgtg
    28741 agttgcctgg atcgggttag taaaaccaca ggcatctttc agggcattag tcgccagaac
    28801 cgcgaaatct tcttctttca cgttcaggtc gcgtaggcct gccgggatat ccactttctt
    28861 cgccagttca cggatggcgt taatgcaggc ttcagcacct tccgcatcgt ttttatctgt
    28921 cacgttcacg cccattgcag cggcacagtc acgcagacgt gcggcggcga ctttgctgtt
    28981 gaatacctga acatgcggca gcaaaacggc gttacataca ccgtgtggca ggttgtagaa
    29041 accgcccagc tggtgcgcca tcgcatgaac ataacccaga gaagcattat tgaacgccat
    29101 accggcgagg aactgggcat aagccattgc ttcacgcgct ttcgcattac tgccatcttc
    29161 aacggctaac ggcaggtttt cggcaatcat ggtcacggct ttcagtgcac aagcgtcagt
    29221 gatcggcgtg gcggcaatag aaacatacgc ttcgatagcg tgcgttaagg cgtccatacc
    29281 agtggcggcg gtcagtgact tcggcatacc gatcatcagc gaggagtcat tgacagaaag
    29341 cagcggagtc acgtgcttat caacaatcgc cattttgata tgacgcgctt cgtcagtgat
    29401 gatgcagaaa cgggtcattt cagaggccgt acccgccgtg gtattgatgg cgatcatcgg
    29461 cagctgcggt tttgcagagc ggtcaacgcc ttcgtaatcg cgaatatcgc cgccattggc
    29521 tgccaccagc gcaatacctt ttgcgcagtc gtgtggagaa ccaccgccta aggagatcac
    29581 gctatcgcaa ttattctctt taagtaattt caaacctgcg gcgacgtttt ccgtggtggg
    29641 gttaggttgg gtgccatcat aaataacgct aaaaatattg cgttcttcca gtgctttttg
    29701 cacatcgccc gccataccta atttcgttaa catactgtca gtgacaatta aggtacgggt
    29761 aaatccataa tctgctatca tattcattgc atcagtcaat gaatcagcgc cgatgacatt
    29821 cacagaagga ataaagaaag ttgaagatgc cataacacac tcctgaaagt gtaaaaaagg
    29881 gtgggcagct tactgaggat tttcattcga aaacatgat
//
LOCUS       DAPHJE010000052        29663 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPHJE010000052 DAPHJE010000000
VERSION     DAPHJE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.052"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(58..1887)
                     /gene="glmS"
                     /locus_tag="RH222_002313"
     CDS             complement(58..1887)
                     /gene="glmS"
                     /locus_tag="RH222_002313"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV3797324.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2049..3419)
                     /gene="glmU"
                     /locus_tag="RH222_002314"
     CDS             complement(2049..3419)
                     /gene="glmU"
                     /locus_tag="RH222_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV3797325.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3770..4189)
                     /gene="atpC"
                     /locus_tag="RH222_002315"
     CDS             complement(3770..4189)
                     /gene="atpC"
                     /locus_tag="RH222_002315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV3797326.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4210..5592)
                     /gene="atpD"
                     /locus_tag="RH222_002316"
     CDS             complement(4210..5592)
                     /gene="atpD"
                     /locus_tag="RH222_002316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV3797327.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5619..6482)
                     /gene="atpG"
                     /locus_tag="RH222_002317"
     CDS             complement(5619..6482)
                     /gene="atpG"
                     /locus_tag="RH222_002317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV3797328.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6533..8074)
                     /gene="atpA"
                     /locus_tag="RH222_002318"
     CDS             complement(6533..8074)
                     /gene="atpA"
                     /locus_tag="RH222_002318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV3797329.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8087..8620)
                     /gene="atpH"
                     /locus_tag="RH222_002319"
     CDS             complement(8087..8620)
                     /gene="atpH"
                     /locus_tag="RH222_002319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV3797330.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8635..9105)
                     /gene="atpF"
                     /locus_tag="RH222_002320"
     CDS             complement(8635..9105)
                     /gene="atpF"
                     /locus_tag="RH222_002320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV3797331.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9167..9406)
                     /gene="atpE"
                     /locus_tag="RH222_002321"
     CDS             complement(9167..9406)
                     /gene="atpE"
                     /locus_tag="RH222_002321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV3797332.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9453..10268)
                     /gene="atpB"
                     /locus_tag="RH222_002322"
     CDS             complement(9453..10268)
                     /gene="atpB"
                     /locus_tag="RH222_002322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV3797333.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10277..10657)
                     /gene="atpI"
                     /locus_tag="RH222_002323"
     CDS             complement(10277..10657)
                     /gene="atpI"
                     /locus_tag="RH222_002323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV3797334.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11274..11897)
                     /gene="rsmG"
                     /locus_tag="RH222_002324"
     CDS             complement(11274..11897)
                     /gene="rsmG"
                     /locus_tag="RH222_002324"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV3797335.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11961..13850)
                     /gene="mnmG"
                     /locus_tag="RH222_002325"
     CDS             complement(11961..13850)
                     /gene="mnmG"
                     /locus_tag="RH222_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV3797336.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14229..14672)
                     /gene="mioC"
                     /locus_tag="RH222_002326"
     CDS             complement(14229..14672)
                     /gene="mioC"
                     /locus_tag="RH222_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV3797337.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14762..15220)
                     /gene="asnC"
                     /locus_tag="RH222_002327"
     CDS             complement(14762..15220)
                     /gene="asnC"
                     /locus_tag="RH222_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV3797338.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15372..16364
                     /gene="asnA"
                     /locus_tag="RH222_002328"
     CDS             15372..16364
                     /gene="asnA"
                     /locus_tag="RH222_002328"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV3797339.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKVLPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16369..17820)
                     /gene="viaA"
                     /locus_tag="RH222_002329"
     CDS             complement(16369..17820)
                     /gene="viaA"
                     /locus_tag="RH222_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV3797340.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17814..19310)
                     /gene="ravA"
                     /locus_tag="RH222_002330"
     CDS             complement(17814..19310)
                     /gene="ravA"
                     /locus_tag="RH222_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV3797341.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19533..21401
                     /gene="kup"
                     /locus_tag="RH222_002331"
     CDS             19533..21401
                     /gene="kup"
                     /locus_tag="RH222_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV3797342.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21568..21987
                     /gene="rbsD"
                     /locus_tag="RH222_002332"
     CDS             21568..21987
                     /gene="rbsD"
                     /locus_tag="RH222_002332"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV3797343.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21995..23500
                     /gene="rbsA"
                     /locus_tag="RH222_002333"
     CDS             21995..23500
                     /gene="rbsA"
                     /locus_tag="RH222_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV3797344.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLGKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23505..24470
                     /gene="rbsC"
                     /locus_tag="RH222_002334"
     CDS             23505..24470
                     /gene="rbsC"
                     /locus_tag="RH222_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV3797345.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24495..25385
                     /gene="rbsB"
                     /locus_tag="RH222_002335"
     CDS             24495..25385
                     /gene="rbsB"
                     /locus_tag="RH222_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV3797346.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25511..26440
                     /gene="rbsK"
                     /locus_tag="RH222_002336"
     CDS             25511..26440
                     /gene="rbsK"
                     /locus_tag="RH222_002336"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV3797347.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26444..27436
                     /gene="rbsR"
                     /locus_tag="RH222_002337"
     CDS             26444..27436
                     /gene="rbsR"
                     /locus_tag="RH222_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV3797348.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27402..28829)
                     /gene="mdtD"
                     /locus_tag="RH222_002338"
     CDS             complement(27402..28829)
                     /gene="mdtD"
                     /locus_tag="RH222_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV3797349.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28852..29544)
                     /locus_tag="RH222_002339"
     CDS             complement(28852..29544)
                     /locus_tag="RH222_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV3797350.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7131 a         7609 c         7584 g         7339 t
ORIGIN      
        1 atgtcagtta taaaaaaggg ccaccggtcg gcagcccttt tttatctatg atagtgatta
       61 ctcaaccgta accgattttg ccaggttacg cggctggtca acgtcggtgc ctttgatcag
      121 cgcgacatgg taagccagca gctgcagcgg aacggtgtag aagatcggtg caatcacctc
      181 ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac ccgcatcctg
      241 atcggcgaag acatacaact gaccgccacg cgcgcgaact tcttcaatgt tggatttcag
      301 tttttccaac agttcgttgt tcggtgcaac gacgataacc ggcatatccg cgtcaatcag
      361 cgccagcggg ccgtgtttca gctcgcctgc agcgtaggct tcagcgtgaa tgtaagagat
      421 ctctttcagc ttcaatgcgc cttccagcgc gattgggtac tgatcgccac ggcccaggaa
      481 cagcgcgtga tgtttgtcag agaaatcttc tgccagcgct tcaatgcgtt tgtcctgaga
      541 cagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacaatgt catgttcaat
      601 ggaggcatcc agacctttca ggcgagacag cttcgccacc agcatcaaca gcacagttaa
      661 ctgagtggtg aatgctttag tggatgccac gccgatttcc gtacccgcgt tggtcatcag
      721 cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg ccagcgaacc
      781 aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg tttcgccaga
      841 ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttgcgat agcggaattc
      901 agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt aacgggaaac
      961 cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa ccttcgacag
     1021 cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc tgatgcgccc
     1081 ggtaagggtg tttttgatcg cgttcggctg ttcgtagatc tctttctgca tgtagtgacg
     1141 gtaaatacct ttatcgcccg cgtcatattg cagattggat tcgatatcct gacgttttac
     1201 ttccgcgcca gttttatcga agatgtttac cgaacggcga gtaatttccg caatatcgcc
     1261 ctcttcaagg aagataaagc gacgggtcac cggcaacagc gccagctggt cagaagcgat
     1321 aaagttttcg cccataccca ggccaatcac cagcggacta ccagaacgtg ccgccagcag
     1381 ggtatccggg tgacgggagt ccatgatcac tgtaccgtac gcaccacgca gttgcgggat
     1441 agcacgcaga acagcctcac gcagagtacc gccctgtttc agctcccagt tcaccagatg
     1501 ggcaattact tcggtgtctg tttcagaaac gaaggtatag ccacgcgctt ttaactcttc
     1561 gcgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt gttcagaaac
     1621 atgcggatgc gcattcactt ctgaaggttc accgtgggtc gcccagcgag tatgagcaat
     1681 acccgtaccg ccatgcagag gatgttcttc cgctgcctga gccagcatct ggactttacc
     1741 gaggcgacgc aggcgggtca tatgaccttc cgcatcaaca acggccagac cggcagagtc
     1801 atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta catcacgttg
     1861 cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc gttgtcggtc
     1921 aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg ttatttttat
     1981 agttactgct tgtgggaggg agattatgtt atctcctcat cccagatgac cggattaccc
     2041 ggccagaatt actttttctt taccggacga cgccagcctt ctttctgagt ctgcggcaca
     2101 cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc cgcagcaatg
     2161 gtcgcgcctt tgcctactgt taccggggcc accagctgag tgtcggaacc gacaaacaca
     2221 tcgtcgccga taatggtctt aaatttattc gcaccatcgt agttgcaggt aatggttccc
     2281 gcgccgatgt taacgttatc gccaatttcc gcatcgccca ggtaagtcag atgaccagct
     2341 ttcgagcctt tacccagacg cgcttttttc atctcaacaa agttaccgac gtgtgcacct
     2401 tccagcaact cagcaccagg acgcagacgg gcaaacgggc caatagtaca ggccgctgcc
     2461 agattcgcgt cttccacaac ggtatacgga ctgatttcgc aatcatcgcc aatcacgctg
     2521 tttttaatca cgcaaccggt gccgattttc acgcgatgac cgagagtcac gttgccctcg
     2581 atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc gcgcagatca
     2641 aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc ctgttcggac
     2701 tggtaaacgc gctccagacg ggagagttgc aggcggttat tcacaccttc tacttcgctt
     2761 aaacgttgcg gatgaacggc gacgatttca cgcccttcct gatacgccag cgcaataatg
     2821 tcggtgatgt agtattcgcc ctgagcatta ttgttggtca gcttcgccag ccagcgtttc
     2881 atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg acgctgctcg
     2941 tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cattttcacg ggtgatacgt
     3001 ccataaccgg tcggatcatc cagtttcacc gtcagcagac caatgccacc ctgcggttta
     3061 gcatcacgca ggcgctggag tgtttcgaca gagatcagcg gcacgtcgcc gtagagcatt
     3121 aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcattgcatg acccgtaccc
     3181 agctgctctg cctgaagcac ccagttaagg ttgtcgtctt tcagcgcctg tttaagcaga
     3241 tcgccgccgt gaccgtacac caggtgaacg tgcgctgcgc ctaattcatt cgcagcatca
     3301 atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg aagatcggaa
     3361 tacatgcgcg tgcctttgcc tgcggcaagg atcactacgc tcatagcatt attcaacata
     3421 cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa aaactacatt
     3481 tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc cccgctgacg
     3541 aaagtgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag gcagtacctt
     3601 catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa acgggaaaac
     3661 aggggtgttt tatatcagca ggatctatgt gaacgctctt caggacggat cacgcgtaaa
     3721 aaaaagccag cctgtttcca gactggcttt tgtgcttttc aagccggtgt tacatcgctt
     3781 ttttggtcaa ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc gcagacgcct
     3841 gagcgtaatc tacgtcgccg tgagagctgc taatgtgctc ttcagcctta cgtttcgctt
     3901 ccatggctcg cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc agaacggtca
     3961 cgttgccagg ctgcacttca agaatgccgc cagacagata gataaactct tcgtgaccgt
     4021 gctgtttcac gatgcgaatc ataccaggct taatggcggt gagcagcggt gcgtggccag
     4081 ggtagatccc cagttcacct tcgctacccg ttacctggat tttctcgacc agaccagaga
     4141 acatttgttg ctctgcgctg acgacgtcca ggtggtaagt cattgccata tcaccctccg
     4201 attaaggcgt taaagttttt tggctttttc cacagcttct tcgatggaac cgaccatgta
     4261 gaacgcctgc tccggcaggt gatcgtattc gccttccatg atgcctttaa agccacggat
     4321 ggtgtctttc agggagacgt atttacccgg agaaccggtg aatacttctg ccacgaagaa
     4381 cggctgggac aggaagcgct ggatcttacg agcacgcgct accaccagtt tgtcttcttc
     4441 agacagttca tccataccca ggatggcgat gatgtctttc agttcctgat aacgttgcag
     4501 gatggactga acgccacgcg cggtgtcgta gtgttcctga ccaaccacca gcgggtccag
     4561 ctgacggctg gtggagtcca gcgggtcaac ggccgggtag atacccagag acgcgatctg
     4621 acggctcagt accacggttg cgtcaaggtg cgcaaaggtg gttgccggag acgggtcagt
     4681 caagtcatcc gcaggtacgt ataccgcctg tacggaggtg atagaaccag ttttggtgga
     4741 ggtgatacgt tcctgcagaa cgcccatctc ttccgccagg gtcggctgat aacctaccgc
     4801 tgaaggcata cggcccagca gtgcggatac ttccgtaccg gccagggtgt aacgatagat
     4861 gttgtcaacg aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag ccatggtcag
     4921 accggtcaga gcaacgcgca gacggtttcc cggcggctcg ttcatctggc catacaccag
     4981 ggatactttg tcgataacgt tggagtcggt catttcgtgg tagaagtcgt taccctcacg
     5041 agtacgttca cctacgcccg caaacacaga gtaaccggag tgctcgatcg cgatgttacg
     5101 aatgagctcc atcatgttta cggttttacc tacacccgca ccaccgaaca gaccaacttt
     5161 accgccctta gcgaacggac acatcaggtc gataactttg ataccggttt ccagcagttc
     5221 ctgagagttt gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc aacgctcttc
     5281 ttcaccgatc tcgcctttca tgtcgaccgg ttcacccagt acgttcatga tacggcccag
     5341 agtcgcttta cctaccggga cttcgatcgg gtgttcgagg tcttttacat ccagaccgcg
     5401 acgcagaccg tcggaggaac ccattgcgat ggtacgcacg ataccgccgc cgagctgctg
     5461 ctgaacttcc agcaccagac gctcattacc attttgcacc tcaagagcat cgtacacgcg
     5521 cggtacggca tcctgaggga attcgacgtc aactacggcg ccgattacct ggacaatctt
     5581 tccagtagcc atcttaaatc ctctacgaaa taacctgttt aaaccgcggc ggcccccgag
     5641 acgatctcgg tgagttcctg agtaatgctg gcctgacgag ctttgttgta taccaactgc
     5701 agctctttaa tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac catacgggcg
     5761 gcctgctcgc tggccaggtt ttcaaccacg ccctgataaa cctgagattc gacataacga
     5821 cgcagcaggg tatccagcaa cgccttcgga tcgggttcgt acaggtaatc ccaggattta
     5881 tgtttcagat catcatcatc tgatgccggt aacggcagca gctggctgat ggtcggaacc
     5941 tgagacatgg tgttaataaa tttgttgctg acaatgtaaa gcttgtccag acggccttcg
     6001 tcgtaggcct gcaacatcac ttttaccgga ccgatcagtt cggacaggga agggttatcc
     6061 cccatgccgg tgacctgggc aacaacattg ccgcccacgg agttgaagaa cgacacgcct
     6121 ttcgagccga tcattgcgag gtcgcattga acgcctttgt cggtccaggt cttcatttcc
     6181 gccagcagtt ttttgaacag gttaatgttc aaaccaccgc acaaaccacg gtcggtcgac
     6241 accaccaggt agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg cttatattcc
     6301 agattaccgt gtgcaaggtg accaatcact ttgcgcatgg tttctgcata aggacggctg
     6361 gccgccatgc gatcctgcga tttacgcatt ttggaagcgg cgaccatctc catcgcttta
     6421 gtgatctttt gcgtgttctg gacgcttgcg atcttactac gtatctcttt tgcgccggcc
     6481 atgagcttct cctcaatgcc ttgcggcctg ccctaaggca agccgccaga cgttaccagg
     6541 attgggttgc tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt tcgtcgttgt
     6601 agccaccggt ctggttgatc tcttgcatca acggagcgtg atcacggtcg acgtaagcca
     6661 gcagagcggc ttcgaagctg ccaattttcg acagttcaac atccgccagg taaccacgtt
     6721 ctgctgcgaa cagaaccaga gactgctgcg caacggacat cggcgcatac tgtttctgtt
     6781 tcagcagttc ggtcactttc tgaccgtggt caagctgctt acgtgttgca tcgtcaaggt
     6841 cggatgcaaa ctgagagaac gctgccagtt cacgatactg tgccagagcg gtacggatac
     6901 caccggacag ttttttcatg atcttggtct gtgctgcacc accaacacgg gatacggaaa
     6961 tacccgggtt aaccgcagga cgaataccgg cgttgaacag gttggtttcc aggaagatct
     7021 gaccatcggt aatggagatt acgttggtcg gaacgaacgc agaaacgtca cccgcctgag
     7081 tttcgataat cggcagtgcg gtcagagaac cggttttccc tttcacttca cctttggtga
     7141 aggcttcaac gtattcggcg ttaacacgcg cagcacgctc cagcagacga gagtggaggt
     7201 agaatacgtc gcccgggaat gcttcacgtc ctggcggacg acggagcagc agggagatct
     7261 gacggtaagc aacagcctgt ttagacaggt catcgtaaat gatcagcgca tcttcaccgc
     7321 ggtcacggaa gtattcgccc attgcgcaac cggcatacgg agccagatat tgcagtgcag
     7381 cggattcaga cgcggttgct accacaacga tggtgttagc cagtgcgccg tgctcttcca
     7441 gtttacgtac cacgttagaa atggtggacg ctttctggcc gatagcgaca tagatacatt
     7501 tgataccgga atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt ttaccggtct
     7561 gacggtcacc gatgatcaat tcacgctgac cacgaccaat tgggatcatg gagtcaacgg
     7621 ctttataacc ggtctgtacc ggctgatcta cggactgacg ttcgataacg cccggagcga
     7681 ttgcttctac agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg attggtgcac
     7741 ccagagtgtt aaccacacgg cccagcaggc cacggccaac cggaacttcc aggatacggc
     7801 cagtacactt aactttcatg ccttcggcaa ggtcagcgta cggacccata acaaccgcac
     7861 ctacagagtc gcgctcgagg ttcagtgcga tagcgtaacg gttacccggc agggagatca
     7921 tttcaccctg catacaatcg gccaggccgt gaatgcggat aacaccgtca cttacagaaa
     7981 caatagtacc ttcgttgtga gcttcactca caacattgaa ctgagcaatg cgctgcttga
     8041 tcagttcgct gatttcggtg gaattcagtt gcatgctcca gtccccttaa gactgcaaga
     8101 cgtctgcaag gcgctcaaga cgaccgcgta cgctgccatc aatgaccata tcacccgctc
     8161 ggatgataac gcctgccatt acagacttat cgattttgca attcagctta actttgcgtg
     8221 acagacgttt ttccatcgca gcagaaattt tcgcgagctg ttgttcactc agtgcggcag
     8281 cggaaatgac gtctacctca gcggtagcct cactcacggc acgaaggtga ataaactgct
     8341 ccagaacatc cgggagcgcg ttaagacgac cattttcagc cattacccga atcaggttct
     8401 gaccgttttc gtccagttgc tcaccacaaa ctgcgataaa cgactcggcg agcgtttctg
     8461 gcgcaagcgc gccagagaga agctctgcca tttgttcgtt tttggttacc tcggcggcaa
     8521 acgccagcat gtcctgccag cgttctacac tttggtgttc gacggcaaag tcaaaagctg
     8581 ctttggcgta ggggcgagct accgtaataa attcagacat cagcccctcc ctccttacag
     8641 ttcagcgaca agtttatcca cgatgtcgct gttagcagct tcatccacgg aacgttcgat
     8701 gatcttctcg gcgccagcaa cagccaggat agcaacttgc ttacgcagct cttcacgggc
     8761 acgtttacgc tcggcttcaa tttctgcctg cgcctgggcc acgattttag tacgttcctg
     8821 ttctgcctca gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg cctgctcgat
     8881 gattacctgg gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg cctttgcaag
     8941 gtcaaggtcc ttatgtgctc gttctgcgga agcaaggccg tcagcaattt ctttttgacg
     9001 tttttcgatg gctgccatta atggcggcca tacgtacttc atgcagaaca gaacgaacag
     9061 gacaaacgcg atggcctggc cgaggattgt tgcgttaaga ttcacagcac aatgcctcta
     9121 tttagttaac gttctgatat tgctctttaa ataaaagcaa cgcttactac gcgacagcga
     9181 acatcacgta cagacccaga cctacagcga tcatcgggat agcatccacc agacccataa
     9241 cgataaagaa ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct gcgccttcca
     9301 ggaatttacc cccgaggatg ccgataccga tcgcagcacc gattgccgcc agacccatca
     9361 tcacagcggc agccatgtac agcagatcca tattcaggtt ttccatgaca gtctccagtt
     9421 tgtttcagtt aaaacgtagt agtgttggta aattaatgtt cttcagacgc catcgacaga
     9481 tagacgatcg tcagaaccat gaagatgaag gcttgcagcg taatgatcag gatgtggaaa
     9541 atggcccacg gcacattcag gatccactgt gaccaccacg gcaacagacc agcaatcaga
     9601 atgaaaatca gctcaccggc atacatgtta ccgaacagtc gcaaaccaag tgaaactggt
     9661 ttggacagca ggcttacccc ttcaaggatt aagttgacag gaatgaacgc ccagtgattg
     9721 aacggctgca gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt gatgctgtag
     9781 aacagaatca ggataaatac gcccaatgcc atagacagcg ttacgttcac gtccgcagac
     9841 ggaaccacac gcagtgcagg cagacccagt acatgttcag caatgtacgg cagcaggtcg
     9901 ataggcagta aatccatcag gttcatcagg aatacccaga cgaagatcgt cagggccagc
     9961 ggagcaatca gcttgctttt gccatggtac atgtctttca cgctaccatt aacaaagccg
    10021 atcaccagct caatcgcggt ctgaaactta cctggcacac cgctggtcgc ctttttggct
    10081 acgctacgga ataaaaccag gaacaacaga cccagcacca ccgagaagaa catggagtca
    10141 atattgattg tccagaaggt ggctgggggg ttttgtggat ccaccagcga gaatgtacgc
    10201 aggtccagct gaaggttatt caggtggtgt cctatgtaat cctgcggcgt catattttct
    10261 gaagccatga tgccttttac cctttgttgt taattacagc cggtgccagt atctgaacca
    10321 ccagcaccaa aacccacgta acgatcagcg gcaagaatac cgcctttaaa accgccaacg
    10381 ccaccaccag taacaccaac atcgccagaa ctttgaaagc ttcgccaaat gcgaatgtcc
    10441 aggccacccg gcctttcgct ggtgtatgcg cctggtgacg ccaggcaaat atcataaaca
    10501 aaacgttagg cagaaagact gccaggcccc cgcttattgc agagacgccc cagaaggggt
    10561 ctttgaggct gaacagcaat ccacttgcta tcaccaccag taactgaacg agcagaagct
    10621 tccgagcaac gtttcgactc acgagcgaca cagacatcac gtttttcact cctgctccct
    10681 tcgaggtatg ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa gcatcaaaaa
    10741 gcggtcaaat tatacggtgc gcccccgtga tttcaaacaa taagtagcca aaaggtgaat
    10801 aaatgtttaa atatttttcc agtgcataca attgcgactt ttctgctaac cctgttcgat
    10861 catgaaaaac tgtaaataac gcgtaaacac tggtgataaa gcgtgcttca gatcacatat
    10921 tgcgcatgtt cgcgcacagc atatttattt acttggcaaa tgatgccttt gcaagtttat
    10981 gatatttcag tctaaaaaca gatactgttt taataaatga catttacaca acaaaaacca
    11041 cccattgaca tttttaataa tgttttaaca gccaatgatg gttcttagcg ccgattttta
    11101 gcagactgat attttcacta atgacttatt ttctgcttac caaaaaaagc cacgttatct
    11161 tgttgatgca aaagagtgaa cgtggcgtta aatgtaacca gttatatcag tagaaaacct
    11221 ggttgttgtt aacagtctaa ccggtcaatt ttttatgatt tttttgataa aaattaaatt
    11281 ttatttgctt taatcaccac cagatgacgt tcgccatcca gggctggaac ctgaagttta
    11341 accactgatt cgacctgata ttcttcgggc aacaaagcga tttcatcttc cggcatttgc
    11401 cctttcagcg cgtagaaacg gccttgctca ccaggaagat ggtggcacca gctcaccata
    11461 tcgttcagag aggcaaaagc gcggctaatt acgccatcaa atggcggctc tgaaggaaac
    11521 tcttctaccc tgctctgtac tggttcaata ttctccagtt taagctcatg ttgcacctga
    11581 cgaaggaaac gcacgcgttt accaaggcta tccaacagag tgaaatgggc ttcaggacgc
    11641 acgatagaga gtggaatgcc tggcagtcct ggtccggtgc cgacatcgat aaaccgttca
    11701 ccttgcagat acggtgccac cacaatgcta tcgagaatat ggcgtaccag catctcatta
    11761 ggatcgcgga ccgaagtcag gttgtacgct ttgttccatt tatgcagcat attcacgtag
    11821 gcaataagct ggtttttctg gtgatcggta agcgaaatac ctgcgtcttt cagcagtaag
    11881 gagagtttgt tgagcacggt gattacctgt tcttgatgcg ttgcctggta agcgggtgct
    11941 taccaggcat ttttaatgcg ttatgcgcta cgacgcagca taccctgttt tttcagccac
    12001 accagcagaa tggagatggc cgcaggcgtg acgccagaaa tacgcgaagc ctggccgata
    12061 gaggctggtt tgtgatcgtt aagtttggcg atcacttcgt tagaaagacc ggatacctgg
    12121 cggtaatcca gtgtcgcggg tagcagggtg ttctcgttac gcagctgctt ttcgatctca
    12181 tcttgctggc gcgcgatata accttcgtat ttaacctgaa tctcaacctg ttccgccgcc
    12241 tgttcgtctg tcaacgcagg ggcaaacggc gtcagcgtgg ttaatttttc ataagtcatt
    12301 tccggacgac gcagcagatc ttcaccactg gcttcacggg aaagcggcgc agtcaggtga
    12361 gcattcactt cggctgcagc ttccgccgac ggggttaccc aggtcgattt cagacgctga
    12421 cgctcacgct cgatattctc aagtttctcg ttaaagcgcg cccaacgttc gtcatccacc
    12481 aggcccagtt cacgaccgat ttcagtcaaa cgcagatccg cattatcttc gcgcagcatc
    12541 agacgatatt ctgcgcgcga ggtaaacata cgatacggtt ctttggttcc taaagtgcac
    12601 aggtcatcaa ccagtacgcc gagatacgcc tgagaacgcg ccggagccca accttctttg
    12661 tcagcagaca gacgggcagc gttaagaccg gccagtaaac cttgcgcagc ggcttcttcg
    12721 taaccggtag tgccgttaat ctgaccagca aagaacagcc cctggataaa cttgctctcc
    12781 agcgtcggtt tcaggtcgcg aggatcgaag aagtcatact caatggcata acccggacgc
    12841 acgatcttcg cgttttccat cccctgcata gagcggacga tttgcatctg cacatcgaac
    12901 ggcaggctgg tggagatacc gttcggataa atttcgttag aggtcagtcc ttccggttca
    12961 aggaagatct gatgctgatt tctgtcggcg aagcgcatga ctttgtcttc gatcgacggg
    13021 cagtagcgtg ggccgacacc ttcgatcacc cctgcataca ttgggctacg atcgaggtta
    13081 ctgcggatca catcatgggt tttctcgttg gtatgagtga tataacacgg cacctgctgg
    13141 ggatgctggg acgcattgcc cataaacgag aataccggca ttgggttatc gccatgctgt
    13201 tgcgccagca cactaaagtc gatagttcga gcatcaatac gcggtggtgt cccggttttc
    13261 agacgaccaa cgcgcagcgg cagttcacgc aaacggcgag aaagcggaat ggacggcgga
    13321 tcaccagcac ggccaccgct gtaattatcc agaccgatat gaattttacc gtcgaggaac
    13381 gtcccaacgg tgagcacgac ggctttggca cggaacttca gtcccatttg ggtaacagca
    13441 ccgaccacgc gatcgttttc gacaataaga tcttcaaccg cctgctggaa gatcatcagg
    13501 ttcggttggt tctccagcgc cgtacgtacc gcctgacggt agagcacacg atccgcctga
    13561 gctcgggtag cgcgaaccgc cggtcctttg cttgcgttta gtatcctaaa ctggataccc
    13621 gcttgatcga tcgctttcgc catcagaccg ccgagtgcat ccacttcttt taccagatgt
    13681 cccttcccaa taccgccgat cgccgggttg cagctcatct gccccagagt gtcgatattg
    13741 tgtgtcaaaa gcagagtctg ttgacccata cgcgccgcgg ccatcgcggc ctcggtgcct
    13801 gcatgacccc cgccaatgat gatgacgtca aaaggatccg gataaaacat ggtgattgcc
    13861 tcgcataacg cggtatgaaa atggattgaa gcccgggccg tggattctac tcaactttgt
    13921 cggcttgaga aagacctggg atcttgggta ttaaaaagaa gatctattta tttagagatc
    13981 tgttctattg tgatctctta ttaggatcgc actgccctgt ggataacaag gatccggctt
    14041 ttaagatcaa caacctggaa aggatcatta actgtgaatg atcggtgatc ctggaccgta
    14101 taagctggga tcagaatgag gggttataca caactcaaaa actgaacaac cgttgttcct
    14161 tggataacta ccggttgatc caagcttcct gacagagtta tccacagtag atcgcacgat
    14221 ctgtatactt atttgagtaa attaatccac gatcccagcc attcttctgc cggatcttcc
    14281 ggaatgtcgt gatcaagaat gttgatcttc agtgtttcgc ctgtctgttt tgcaccggaa
    14341 tctttgagtt ctgcctcgag tttatcgata gccccacaaa aggtgtcata ttcacgacta
    14401 ccaataccga ttgcgccaaa gcggaccgca gaaagatcgg gcttctgttc ctgcaatgct
    14461 tcatagaaag gagaaaggtt gtccggaata tctccggcac cgtgggtgga gctgataatc
    14521 agccagatcc ccgaggcaga taaatcttcc aacagcggac cgtgcagcgt ttcggtggta
    14581 aaacccgcct cttccagctt ttcagccagg tgttctgcta catattcggc accgccgagg
    14641 gtgctgccgc tgataagagt gatatctgcc ataaaccgcc acctttatta agagtggcgt
    14701 attgtacgct gtgaacgcgt tgggatctac ctgtggaaaa gtatgggatt aaaaaagccg
    14761 atcagggctt gatagtacgc atgatcgggt tttgcaggac gatcaatgtc tcggtggact
    14821 gaatttcatc aattgtttgg atcttgttga taagtacatg ctggagagcg tcgatcgaac
    14881 ggcacattac ttttataaag atgctgtagt ggcctgttgt gtaataggct tcagtgactt
    14941 catcaaggct ttccagcttt gccagcgcgg aagggtagtc tttggcgctc tttaatataa
    15001 tgccgataaa gcagcctacg tcataaccga gctgcttcgg gctgacatca atacgcgccc
    15061 cggtaatgat ccccgcctgc ttcattttct ctacccgaac gtgaatcgtc cccggactga
    15121 cgccaaattg tttcgccagt tcggcgtaag cggtgcgcgc attgcccatt aatgcttcca
    15181 ggatgccacg gtccagattg tcgatcagat aattttccat aggattttct tatgcagatt
    15241 gatgattcat tgtattttag ccttcttttt taatgaatca aaagtgtgtt aggcttttta
    15301 ttgaatgatt attgcatgtg tgtcggtttt tgttgcttaa tcataagcaa caggacgcag
    15361 gagtataaaa aatgaaaacc gcttacattg ccaaacaacg ccaaattagc ttcgtgaaat
    15421 ctcacttttc tcgccaactg gaagaacgtc tggggctgat cgaagtccag gcgccgattc
    15481 ttagccgtgt gggggatggc acgcaggata acttgtcggg ctgtgaaaaa gcggtgcagg
    15541 taaaagtgaa agttctgcct gatgcccagt tcgaagtggt tcattcactg gcgaagtgga
    15601 aacgtcagac cttagggcaa cacgacttca gcgcgggcga agggctgtac acgcacatga
    15661 aagcccttcg ccccgatgaa gaccgtcttt ctccgttgca ctcggtctat gttgaccagt
    15721 gggactggga acgcgtaatg ggcgacggtg agcgtcaatt ctcgactctg aaaagcacgg
    15781 tagaggcgat ctgggcggga attaaagcaa ccgaagctgc ggttagcgaa gagtttggcc
    15841 tggcaccgtt cctgccggat cagatccact tcgtacacag ccaggagtta ctgtctcgtt
    15901 atccggatct tgatgccaaa gggcgtgagc gggcgatagc gaaagatctt ggcgcggtat
    15961 tccttgtcgg gattggcggc aagctgagcg atggtcatcg ccacgacgtg cgcgcaccgg
    16021 attatgatga ctggagcacc ccgtcagagc tgggtcatgc gggtctgaac ggcgatattc
    16081 tggtgtggaa ccctgtactg gaagatgcgt ttgagctttc ttccatgggg atccgcgtgg
    16141 atgccgacac gttgaagcat cagttggcgc tgaccggtga tgaagatcgt ctgcagctgg
    16201 aatggcatca ggcgctgctg cgcggtgaaa tgccgcagac catcggcggc ggtatcggcc
    16261 agtctcgttt gactatgctg ctgctgcaac tgccgcatat cggccaggtt cagtgtggag
    16321 tatggccagc tgctgttcgc gagagcgtcc cttctctgct gtaataattt atcgccgcca
    16381 gcgtctgagc aggcggcttc gcatcccggt atcaaagcgc cagatatgat cgaaaatgcg
    16441 catgatgccg ggtttgccgt gtgccgacat cgccacggca tgaaagcgat gctgatgtac
    16501 ccgctgcagc tctttcactt tactcgtcac gtcgtcaggc aaccgctgag cgataaaatc
    16561 agaaatcacc accgcatcgg catcaaacca ttccctgctt tgcaagcgtt ccataatggc
    16621 gcgaaaacaa ctggcaagat cggtgccgcc acgaaactgc tggcttaaaa aacggattgc
    16681 ttgttcgatg ccttgtgggc ctgaaagctc ataacggacg atctcggtgg aaaatagcat
    16741 aatatagcag cgccggtttt ctgcgagagc aatgcgcatc aaggccaggc agaacgcttt
    16801 cgcacactgt tcattaaagc cgcccattga gccggaagta tccacacaga caataaacgg
    16861 cccgcgcggc tgttcatcgt aatctttatg taccaccgga cgttcgatca ctttttcacg
    16921 ccacgactca ccgtgcaggc gataggtgag caactgtttt tccaccagcc gacggtaaaa
    16981 ctcatactcc agttccgtta tccctagtgt cgccagttct ggcggcagga gacgtaaaat
    17041 atcatcgctt tgttgcagac catcaacctg ctcaggaacc gtcgccggtt cgcgcaccat
    17101 ggtgcggaag gtttccatct gcgcatcgtt gcgcggtatt gatttggctt cccgagaacg
    17161 ccccagctgc tctgccaggc gtttcagttc cggctgttcg ttaagaaatt caccgtattt
    17221 cacaatcaac tgatagtcgc cacgtttaag ctgaccggcg ctcatatccc acagacgacc
    17281 agctgcagta ttgttatctg cgagaatcgg ttcaagttgt ccgctcagcg tcatgcgttc
    17341 ctgaacttca ctcaacagtt gttcgcgttc ttcttctaat agctgttgat ttaacgtcgt
    17401 tgcttgcacg atcagactta aacgccaacg ctggagaaaa agcgtgtgta acgctgaagt
    17461 gatcgtgctg ttagcatcaa ccaactgtcg ggcttgttct gcccatggag aattcagacg
    17521 atgcagtaag tccaggatct gtggtagctg cacaataaac tgtggcgtgg agaggagctg
    17581 gctttgctga tagcacatca cctcttcggt gagttccggc gggactcggg catctttcag
    17641 ccgactgcgc agcgcttcac gccagcgggg aacatcatca gtgattgccg ccttcaggcg
    17701 tgggaatttt tcaaagaaga ccgccagctg cggtgaggcc agcagtgcga tgatcatctc
    17761 ttcgatcaat ccctcttcgc tgacggccag catcacatta agcgtatcca gcgttagcat
    17821 tgttgtgcct ggcgaatctg tgcaccgaca tcctgtaggc tggcttcaat gcgacctaac
    17881 cagtcgccag ggataaacag acatttttgc tgttcgctga ataaagcgtg ttgcttgcgc
    17941 cagtcgcttt caagctcttc cagttgttgt tttatttcac caggcagacc ttcagccgat
    18001 gaaccgggaa gtgccagcgt actgccttgt aaacttacat cgcgaacgac aagatgttgg
    18061 gcgctatcaa cttccagatt cagtttctgg gcaaagccga taccgttcag tttaccgcga
    18121 atttcaccac ctttgctcag ccactgttcc agcgcgctac gctcaaagga gatatgaacc
    18181 acttccatat catgcagttt taacggtttt tgcagcagta gagtcagagt ggaagcagta
    18241 acgttaacag gaagttgata ctgctgacga cggctgaaaa tgccgcccag acgaattacc
    18301 gttaaggctg ttttatcgct ttgttgctgc tggagttgca ggtgacgttg cacaatcgcg
    18361 ccaaggcgag tcaacatccc ttgctgttgc caggcgtgac cggtcatcaa tacatcaatt
    18421 tgttgttgta tcaaattcag gctttgcgcg tcataccaca ggcaatcttt cagcaaaatg
    18481 agatctaccg gggcaacagc actgcgaccg ctaaaaaagg cgctggcctg caataatcgg
    18541 atcgcttttt tccagcgacg atccgagaca taaggcgcat ccggtaattt atccagttgc
    18601 tggcgcagca taaaaatcag ctcaaataca tgatcgggca gcgtaatttc accaatctct
    18661 ttctgccagc gttcatattc ttcatctgtg acctgcaagg catcaggaac cggattgtcg
    18721 ttttcatcct gttgactggt cagcatggag cggaaattcg ctttatcctg cactttatct
    18781 aaccacagac gaatcagcat gcggtcatat aacgcttcca gactgctgtc tgcttccggc
    18841 agctcgttgg aggccgccac cagcagacgc atcgggattt tttctacgtg tgcaccgttg
    18901 cggaactggc gctcgttaat ggcggtgagc aaggtattaa gaattgccgg gcccgctttc
    18961 cagatctcat ccagaaagac aatttctgct tccggcaggt aaccgctggt taaacgttca
    19021 tagcgccctt catcttttag cgcctgaata gaaaggggac caaaaacttc ttccggcgtg
    19081 gagaagcggg tcatcagata ttcaaacgcg cgggcattct gaaaggcgaa ttttaagcgc
    19141 cgggcgatca aacttttggc aatacctggc gggccaagga ggaacacact ttcaccactt
    19201 aatgccgcta ataaacacaa gcggatggcg tggctacgtt cataaagccc cttttccaac
    19261 gaactgctca ggcgggaaat tctttccgct aataaatgag ggtgagccat aatgaagtgg
    19321 cgtcctttcg tcaaaagttc tgcgtaaatt gcgagtatag acgtttcttg ctggtggcta
    19381 aaatagtctc aaaggggggg tatttttctt tgagccaggt taatgtggcc gcatttagga
    19441 gtacgatttt gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc
    19501 acacatttca tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc
    19561 ccgcgattac cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat
    19621 atacacttcg tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt
    19681 ttggcttttt atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca
    19741 ccttcgtgat gcgggcagat aacgccggtg agggggggat cctgacgttg atgtcgcttg
    19801 ccgggcgtaa tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg
    19861 gcagcttttt ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg
    19921 aaggtctgga aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg
    19981 ttctcacttt attatttatg attcaaaaac atggcacggc tatggtcggt aagctgtttg
    20041 cgccgatcat gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg
    20101 ctaacccgga agtgctgcat gcgctgaatc caatgtgggc ggtgcatttc ttccttgaat
    20161 acaaaacggt ttcttttatt gcattagggg cagtggtgtt gtcgattacg ggggtcgagg
    20221 cgctgtatgc tgatatgggg cactttggta agttccctat tcgcctggcg tggttcaccg
    20281 tcgtattacc ttccttgacc cttaattact tcggtcaggg agcgctgttg ttaaagaacc
    20341 cggaagcgat taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccgctgc
    20401 tgatcatcgc cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct
    20461 cattgacgcg tcaggcggta cgtctgggat atttatcgcc gatgcgcatt attcacacct
    20521 ccgaaatgga gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg
    20581 tcgtgattgt gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg
    20641 cggtgaccgg aaccatggtg ctgacgtcta ttctctcgac taccgtggca cgtcagaact
    20701 ggcactggaa taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc
    20761 cattgttcac cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg
    20821 gtacggtgat gtttatcgtg atgaccacct ggaagagcga gcgtttccgc ttgctgcggc
    20881 ggatgcatga acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc
    20941 ctgttcgcgt gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg
    21001 cgctgatgca taaccttaaa cataacaagg tattgcatga gcgggtgatt ctgttaactc
    21061 tgcgtaccga agacgcgcca tatgtccata atgtccgtcg ggtacagatt gaacaactgt
    21121 cgcccacttt ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag
    21181 aagttttcca ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct
    21241 ttatgtcgca tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca
    21301 agctgtactt gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc
    21361 caaacagggt tatcgaactg ggtactcagg tcgaaatcta acgccagacg cctcctttct
    21421 ccataagggg gcgtttttgt tttcatggtt aatcaccatg taaaacgttt cgaggttgat
    21481 cacatttccg taacgtcacg atggttttcc caactcagtc aggattaaac tgtgggtcag
    21541 cgaaacgttt cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt
    21601 tcatcggtga tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta
    21661 cccatctcca aaagtacaac gcgtatcgat atggcattaa cccagggtgt accttctttt
    21721 atgcaggtgc tgggcgtcgt tacaaatgaa atgcaggtcg aggctgccat tatcgcggaa
    21781 gagatcaaac aacataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg
    21841 caaaaacacc agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa
    21901 caaaccgcag aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc
    21961 attctctgtg ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg
    22021 catcgataaa gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa atgtctatcc
    22081 gggccgcgtg atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt
    22141 gcttactggt atctatgctc gcgatgccgg cacgctttta tggctgggga aagaaacgac
    22201 atttaccggg ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa
    22261 cctgatcccg cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg
    22321 ctttggcaaa attgactgga aaaccatgta tgccgaagcg gataaattgc tggctaaact
    22381 taacctgcgc tttaaaagcg acaagctggt gggcgatctt tccatcggtg accagcaaat
    22441 ggttgaaatc gccaaagtgc tgagctttga gtcgaaagtc atcattatgg atgaaccgac
    22501 cgatgcgctg accgataccg aaaccgaatc cctgttccgc gtcatccgcg aactaaaatc
    22561 gcaaggccgc ggtattgtct atatctccca ccgcatgaaa gaaatcttcg agatttgcga
    22621 tgacgttacc gtttttcgtg acgggcaatt tattgctgag cgcgaagtgg catcactgac
    22681 cgaagattcg ctgattgaga tgatggtggg tcgcaagctg gaagatcaat atccgcacct
    22741 gggcaaagcg ccgggagata tccgcctgaa agtcgataat ctctgcggac ctggcgttaa
    22801 cgatgtctct tttactttac gcaaaggcga aattcttggc gtctctggtt tgatgggcgc
    22861 aggtcgtacc gaactgatga aagtgctcta cggcgcacta ccgcgcacca gcggttacgt
    22921 caccctggat gggcatgaag tcgttacccg ttcaccgcag gatggcctgg caaacggcat
    22981 tgtgtatatc tccgaagacc gtaaacgtga cggtttagtg ttgggcatgt cagtaaaaga
    23041 gaacatgtcg ctgacagcgc tgcgctactt cagccgcgct ggcggcagtt tgaagcatgc
    23101 cgatgaacag caggctgtga gtgatttcat tcgtctgttt aatgtgaaaa caccgtcgat
    23161 ggaacaggca attggtctgc tttccggtgg caatcagcaa aaagtggcga ttgcccgcgg
    23221 tctgatgaca cgccccaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg
    23281 cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat
    23341 tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca
    23401 tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc
    23461 tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg
    23521 tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc
    23581 tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact
    23641 tattcaatat tcttcagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg
    23701 tgatcctgac gtcgggtatc gacttgtcgg taggttctct gttggcgctg accggcgcag
    23761 ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gccgctctcg
    23821 cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg
    23881 cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca
    23941 acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta
    24001 ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg
    24061 cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg
    24121 aagcagcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc
    24181 tttgtggtct gctggcatcg ctggccggga tcattgaagt ggcgcgtctc tcctccgcac
    24241 aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta
    24301 cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg
    24361 gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca
    24421 aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac
    24481 aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa
    24541 gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcactggtg gtctccacgc
    24601 ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg
    24661 gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc
    24721 aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag
    24781 taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc
    24841 aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa
    24901 tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag
    24961 gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg
    25021 ctcacaagtt taatgttctt gccagccagc cagcagattt tgatcgcact aaaggtttga
    25081 acgtaatgca gaacctgttg accgctcatc cggatgttca ggctgtattc gcgcagaatg
    25141 atgaaatggc gctgggcgcg ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg
    25201 tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt gaatgatggc aaactagcag
    25261 cgactatcgc tcagctaccc gatcagattg gcgcgaaagg cgtcgaaacc gcagataaag
    25321 tgctgaaagg cgagaaagtt caggctaagt atccggttga tctgaaactg gttgttaagc
    25381 agtagtttta atcaggttgt atgacctgat ggtgacataa atacgtcatc gacagatgaa
    25441 cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac acggtggcgc attttatgga
    25501 catcccgaat atgcaaaacg caggcagcct cgttgttctt ggcagcatta atgctgacca
    25561 cattcttaat cttcaatctt ttcctactcc aggcgaaacc gtaaccggta accactatca
    25621 ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa
    25681 tatcgcgttt attgcctgta cgggtgatga cagcattggt gagagcgttc gccagcagct
    25741 cgccactgat aacattgata ttactccggt cagcgtgatc aaaggcgaat caacaggtgt
    25801 ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa
    25861 tgctgccctt tccccggcgc tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc
    25921 attattaatg cagctggaat caccactcga aagtgtgatg gcagcggcga aaatcgccca
    25981 tcaaaataag actatcgttg cgcttaaccc ggctccggct cgcgaacttc ctgacgaact
    26041 gctggcgctg gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat
    26101 tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg aaaaaggtat
    26161 ccgtactgta ctgattactt taggaagtcg tggtgtatgg gctagcgtga atggtgaagg
    26221 tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc attgctgccg gagatacctt
    26281 taacggcgcg ttaatcacgg cgttgctgga agaaaaacca ttgccagagg cgattcggtt
    26341 tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg
    26401 gcgtgaagag atcgacgcat ttttagacag gcagaggtga cgcttggcta caatgaaaga
    26461 tgttgcccgc ctggcgggcg tttctacctc aacagtttct cacgttatca ataaagatcg
    26521 cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg attaaagaac tcaattacgc
    26581 gccatcagcc ctggcgcgta gcctcaaact caatcaaaca cataccattg gcatgttgat
    26641 cactgccagt accaatcctt tctattcaga actggtgcgt ggcgttgaac gcagctgctt
    26701 cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg
    26761 caatctggaa acgctgatgc aaaaacgcgt tgatggcttg ctgttactgt gcaccgaaac
    26821 gcatcaacct tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg tgatgatgga
    26881 ctgggctccg ttcgatggcg acagcgatct tattcaggat aactcgttgc tgggcggaga
    26941 cttagcaacg caatatctga tcgataaagg tcatacccgt atcgcctgta ttaccggtcc
    27001 gctggataaa actccggcgc gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc
    27061 gggtctcagc attcctgatg gctatgaagt cactggtgat tttgaattta acggcgggtt
    27121 tgacgccatg cgccaactgt tatcacatcc gctgcgtcct caggccgtct ttaccggaaa
    27181 tgacgctatg gctgttggcg tttaccaggc gttatatcag gcagagttac aggttccgca
    27241 ggatatcgcg gtgattggct atgacgatat cgaactggca agctttatga cgccaccatt
    27301 aaccactatc caccaaccga aagatgaact gggggagctg gcgattgatg tactcatcca
    27361 tcggataacc cagccgaccc ttcagcaaca acgattacaa cttactccga ttctgatgga
    27421 acgcggttcg gcttagattt acgctgtctt ttgatcaaat tattaccatc ggttgttttc
    27481 agaagcatga acattgctgc tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag
    27541 tggaattgtt cgacagtcgt tgtgccttcc atcccttcat aaacgcgaag gacggccgca
    27601 cttacagcaa cgcctaaact aatcgacagt tgctgcgtga ccgccagaac actgttaccg
    27661 ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg tattcatcgc ggtaaattgc
    27721 gtcgacatag ccatccctaa tataaacaac ggcaagatca gcatccatat cgccattgcc
    27781 ggtgattgca aagagaactg agcgatcatt agcccaataa tcaccgtgat ccccactaac
    27841 gtatggcgat agcccagacg acgtaagact tgggtaacca tcgattttgc aataatggaa
    27901 cctaacgctg tcggtgccat catacagccg gcaataaacg cctgataacc aaatcctacc
    27961 tgtaacatca atggcataag gaacggtaca caaccggtcc ccagacgggt tgcaatattg
    28021 cctacgatac cgatcgagaa agtgcgggtt ttaaataaat ctaatgaaat taatgggttt
    28081 ggcgtgtgtc gcgcatggag aatatagaga agcagtaacc cgatgctggt gacaattacc
    28141 gtcaaggcaa tccagctggc gacaatcttt tccccgaata gctctattcc gcttgagaag
    28201 agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg
    28261 gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg caatacctat ggggatattg
    28321 attaaaaata tccagtgcca ggttgcccag gtgaccagca cgccgccaag aacggggcct
    28381 aaaattggcc ccaccagacc cggcatggcg acaaaattca atactggaag aagttcatta
    28441 cgaggataag cgcgcagtaa ggccagccga gcaacaggca tcatcattgc gccgcctatc
    28501 ccctgaataa cccggaagac aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga
    28561 gaacccaatg tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg
    28621 gctagccatc cgcttaccgg aataagcatc gccaccgtca gcgtataact gatgatggct
    28681 gattgcatcg cgagaggaga acgattaagg ctatgagcga ttgcgggtaa ggcggtattc
    28741 agaatagtgg catcaagtgc ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc
    28801 gccatactgc gattcttttt atcgctcatt caatgtcctg ttatcgggtt gtcacttatc
    28861 aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc tttggacaat
    28921 cgcatcgaca atcgcctggt gcagatccag ctttatcact gtgtcgctgg taattgacgt
    28981 gaagtaagtg tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa acggattggc
    29041 gctcatttca tagatatgct catgccaggc catatcgact tcgatccagc gttcacggcg
    29101 aaagttctct tttaatgccg ccatttcggc cattaacgta ttgagatgcg ccttctgttc
    29161 cgcggtgcca accgttgcag ccagtaggca agcttgcggc tccagacaga tacgcataac
    29221 cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc atccaccagg taagcaattc
    29281 ctgatcaaga aaattccagt ttgattgtgg catgacccgg gtaccaattc gcggtcgcgg
    29341 taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac
    29401 tccaaattgc tcgcccagct caatctcacc aggcaaaatg gtgccgggtt catattcacc
    29461 ttttaagatg cgttgcgcca gcttctcagc cagaacatac gaaaggtttt tctgtgcagc
    29521 taactgttgt gcgcttaaag gcattactaa tcttcctttt tctttttatt cctccttagt
    29581 atgccaccag gaagtgtgat tacggttgca aaaacggcaa attgcttgtt ttatggcaca
    29641 ttaacggggc ttttgctgaa aaa
//
LOCUS       DAPHJE010000053        29369 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPHJE010000053 DAPHJE010000000
VERSION     DAPHJE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.053"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            73..1026
                     /locus_tag="RH222_002340"
     CDS             73..1026
                     /locus_tag="RH222_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3797351.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1067..2062)
                     /gene="pgl"
                     /locus_tag="RH222_002341"
     CDS             complement(1067..2062)
                     /gene="pgl"
                     /locus_tag="RH222_002341"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV3797352.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2217..3035
                     /gene="ybhA"
                     /locus_tag="RH222_002342"
     CDS             2217..3035
                     /gene="ybhA"
                     /locus_tag="RH222_002342"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV3797353.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3036..4094)
                     /gene="modC"
                     /locus_tag="RH222_002343"
     CDS             complement(3036..4094)
                     /gene="modC"
                     /locus_tag="RH222_002343"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV3797354.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4097..4786)
                     /gene="modB"
                     /locus_tag="RH222_002344"
     CDS             complement(4097..4786)
                     /gene="modB"
                     /locus_tag="RH222_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV3797355.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4786..5559)
                     /gene="modA"
                     /locus_tag="RH222_002345"
     CDS             complement(4786..5559)
                     /gene="modA"
                     /locus_tag="RH222_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3797356.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQNI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVAIFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5726..5875)
                     /gene="acrZ"
                     /locus_tag="RH222_002346"
     CDS             complement(5726..5875)
                     /gene="acrZ"
                     /locus_tag="RH222_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV3797357.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6004..6792
                     /gene="modE"
                     /locus_tag="RH222_002347"
     CDS             6004..6792
                     /gene="modE"
                     /locus_tag="RH222_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV3797358.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6860..8332
                     /gene="modF"
                     /locus_tag="RH222_002348"
     CDS             6860..8332
                     /gene="modF"
                     /locus_tag="RH222_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV3797359.1"
                     /translation="MSSLQILQGAFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8593..9609
                     /gene="galE"
                     /locus_tag="RH222_002349"
     CDS             8593..9609
                     /gene="galE"
                     /locus_tag="RH222_002349"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV3797360.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9619..10665
                     /gene="galT"
                     /locus_tag="RH222_002350"
     CDS             9619..10665
                     /gene="galT"
                     /locus_tag="RH222_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV3797361.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10669..11817
                     /gene="galK"
                     /locus_tag="RH222_002351"
     CDS             10669..11817
                     /gene="galK"
                     /locus_tag="RH222_002351"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV3797362.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11811..12851
                     /gene="galM"
                     /locus_tag="RH222_002352"
     CDS             11811..12851
                     /gene="galM"
                     /locus_tag="RH222_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV3797363.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13056..13808
                     /gene="gpmA"
                     /locus_tag="RH222_002353"
     CDS             13056..13808
                     /gene="gpmA"
                     /locus_tag="RH222_002353"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV3797364.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13966..15018)
                     /gene="aroG"
                     /locus_tag="RH222_002354"
     CDS             complement(13966..15018)
                     /gene="aroG"
                     /locus_tag="RH222_002354"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV3797365.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15334..15714
                     /locus_tag="RH222_002355"
     CDS             15334..15714
                     /locus_tag="RH222_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV3797366.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15827..16768
                     /gene="zitB"
                     /locus_tag="RH222_002356"
     CDS             15827..16768
                     /gene="zitB"
                     /locus_tag="RH222_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV3797367.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16765..17484)
                     /gene="pnuC"
                     /locus_tag="RH222_002357"
     CDS             complement(16765..17484)
                     /gene="pnuC"
                     /locus_tag="RH222_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV3797368.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17512..18555)
                     /gene="nadA"
                     /locus_tag="RH222_002358"
     CDS             complement(17512..18555)
                     /gene="nadA"
                     /locus_tag="RH222_002358"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV3797369.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18804..18929)
                     /locus_tag="RH222_002359"
     ncRNA           complement(18804..18929)
                     /ncRNA_class="other"
                     /locus_tag="RH222_002359"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18960..19035)
                     /locus_tag="RH222_002360"
     tRNA            complement(18960..19035)
                     /locus_tag="RH222_002360"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19000..19002),aa:Lys,seq:ttt)
     gene            complement(19069..19144)
                     /locus_tag="RH222_002361"
     tRNA            complement(19069..19144)
                     /locus_tag="RH222_002361"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19109..19111),aa:Lys,seq:ttt)
     gene            complement(19291..19366)
                     /locus_tag="RH222_002362"
     tRNA            complement(19291..19366)
                     /locus_tag="RH222_002362"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19331..19333),aa:Lys,seq:ttt)
     gene            complement(19370..19445)
                     /locus_tag="RH222_002363"
     tRNA            complement(19370..19445)
                     /locus_tag="RH222_002363"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19410..19412),aa:Val,seq:tac)
     gene            complement(19595..19670)
                     /locus_tag="RH222_002364"
     tRNA            complement(19595..19670)
                     /locus_tag="RH222_002364"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19635..19637),aa:Lys,seq:ttt)
     gene            complement(19673..19748)
                     /locus_tag="RH222_002365"
     tRNA            complement(19673..19748)
                     /locus_tag="RH222_002365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19713..19715),aa:Val,seq:tac)
     gene            complement(19884..19959)
                     /locus_tag="RH222_002366"
     tRNA            complement(19884..19959)
                     /locus_tag="RH222_002366"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19924..19926),aa:Lys,seq:ttt)
     gene            complement(20124..20915)
                     /gene="cpoB"
                     /locus_tag="RH222_002367"
     CDS             complement(20124..20915)
                     /gene="cpoB"
                     /locus_tag="RH222_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV3797370.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20925..21446)
                     /gene="pal"
                     /locus_tag="RH222_002368"
     CDS             complement(20925..21446)
                     /gene="pal"
                     /locus_tag="RH222_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV3797371.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21481..22773)
                     /gene="tolB"
                     /locus_tag="RH222_002369"
     CDS             complement(21481..22773)
                     /gene="tolB"
                     /locus_tag="RH222_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV3797372.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22906..24171)
                     /gene="tolA"
                     /locus_tag="RH222_002370"
     CDS             complement(22906..24171)
                     /gene="tolA"
                     /locus_tag="RH222_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV3797373.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24236..24664)
                     /gene="tolR"
                     /locus_tag="RH222_002371"
     CDS             complement(24236..24664)
                     /gene="tolR"
                     /locus_tag="RH222_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV3797374.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24668..25360)
                     /gene="tolQ"
                     /locus_tag="RH222_002372"
     CDS             complement(24668..25360)
                     /gene="tolQ"
                     /locus_tag="RH222_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV3797375.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25357..25761)
                     /gene="ybgC"
                     /locus_tag="RH222_002373"
     CDS             complement(25357..25761)
                     /gene="ybgC"
                     /locus_tag="RH222_002373"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV3797376.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25911..26204)
                     /gene="ybgE"
                     /locus_tag="RH222_002374"
     CDS             complement(25911..26204)
                     /gene="ybgE"
                     /locus_tag="RH222_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV3797377.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26204..26317)
                     /gene="cydX"
                     /locus_tag="RH222_002375"
     CDS             complement(26204..26317)
                     /gene="cydX"
                     /locus_tag="RH222_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV3797378.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26332..27471)
                     /gene="cydB"
                     /locus_tag="RH222_002376"
     CDS             complement(26332..27471)
                     /gene="cydB"
                     /locus_tag="RH222_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV3797379.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27487..29055)
                     /gene="cydA"
                     /locus_tag="RH222_002377"
     CDS             complement(27487..29055)
                     /gene="cydA"
                     /locus_tag="RH222_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV3797380.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGVSAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7314 a         7774 c         7226 g         7055 t
ORIGIN      
        1 gttggctaaa tgcagttaaa atttttaacg accagccacc caaaatcgtc gcttccattt
       61 tccggagtaa taatgaagca tgaattatca agtatgaagg catttgtcat actggcagag
      121 tccagttcat ttaacaatgc tgctaaatta ctcaatatta cgcaacctgc attaacgcga
      181 agaataaaaa agatggagga agatttacat attcaactat ttgagcgtac aactcgcaag
      241 gttacgttaa caaaagcagg aaaaaggttg ctcccggagg cgcgggaatt aataaagaaa
      301 ttcgatgaaa cgctttttaa tattcgtgat atgaatgctt atcatcgtgg tatggtgacg
      361 ttagcgtgta ttccgaccgc agtattttat tttttaccgc tggcaatcgg taaatttaat
      421 gagctgtatc ccaatattaa agtgcggatt ctggaacaag gcacgaataa ttgcatggaa
      481 tcggtgctgt gtaacgaatc agactttggc ataaacatga acaacgtcac aaattcatcc
      541 attgatttta ccccactggt caacgagcca ttcgtgctgg cctgtcggcg tgaccatcca
      601 ttggccaaaa agcagcttgt agaatggcag gagttagtgg gttacaaaat gattggcgta
      661 cgttcctcca gcggcaaccg attgttgata gaacaacagc tagccgacaa gccctggaag
      721 ctggactggt tttacgaagt gcgtcatctt tcgacgtcat taggactggt agaggcggga
      781 ctggggattt cggcgcttcc tggtctggca atgccccatg cgccctattc ttcgattatt
      841 ggtattccac tggtggaacc cgttatacgt cggacattgg ggattattcg ccgcaaagat
      901 gccgtgcttt ctccggcagc agaacgcttt tttgccttgc tgattaattt gtggactgac
      961 gataaagaca atttgtggac caatatcgtc gaacgccaga gacatgcgtt acaggagata
     1021 ggttaataca ccggcgcagg agagactgcg ccggttaaat cagcggttag tgtgcgttaa
     1081 ccaccaccca cattggtccc tgcccgaccg cataacggcc tttttcatgc agtagtccct
     1141 gctcgccaac aatttcgtat accgagatgt ggtgagattt ttgcccggcg gcaatcagat
     1201 acttgccgct gtgatcaaca ttgaagccgc gcggctgggt ttccgttggc tggaagcctt
     1261 ctttactcaa cacgctgcca tcttccgaaa cgctgaaaac ggtaatcagg ctggcggtac
     1321 ggtcgcaggc gtataaatgg cgaccatccg gggtgatatg aatatcagcc gcccaacggg
     1381 tgtcggagaa gttttccggc atcatatcca gcgtctggac acattcgata ttaccgtgcg
     1441 gatctttcag ttcccagaca tccactgagc tgtttaactc attgacgcaa tacgcatatt
     1501 gttcgtttgg atggaatacc atatgacgcg ggccggcccc ttcaacggtg gtcacttccg
     1561 cagggtcctg cgccacgaga tgaccatcat cgctgaccgt aaacaggcaa atgcgatcct
     1621 gctttaatgc cggaacccac agcgtacggt tgtccggtga gatattggcg gaatggcaac
     1681 cgtccagccc ctcgaccaca tcgacgacgc ccactggcag gccatcttcc agacgcgtta
     1741 cgctcacgtt acccgcattg taagaaccta caaagacaaa ctgcccctgg tgatcggtgg
     1801 aaatatgcgt cggactaccc ggcagcgcag actctgcggc aaaggtcagt gcgccatcgt
     1861 ccggggcgat actatacgcc aggacgcgaa actcagggcg aacaccaaca tagagataac
     1921 gtttgtccgg gctgaccacc atcggctgca cctgccccgg cacatcgaca acctgtgtca
     1981 gcgtcagtgc gccttcatga ttcaggttcc agacgtgaat ttgctggctc tcagggctgg
     2041 cgatataaac tgtttgcttc atgaatgctc ctttgcattt agcaactgac tgtaacagct
     2101 aaaattagtc gcttttggcg gtgaatgctg aattaaaacg cagaattttg tccgatagtg
     2161 gtatcggtgt accattcgcg cagacgaaaa ttcttaacct taatctggaa tacgccatga
     2221 ccacacgcgt gattgctctc gacttagacg gcaccttatt gaccccgaaa aagaccctgc
     2281 ttccttcatc gatagaagcc ctggcccgcg ctcgcgaagc aggctatcaa ttaatcatcg
     2341 tcacaggtcg ccatcacgtc gctattcatc ctttttatca ggcgctggcg ctggatacac
     2401 ctgctatttg ctgtaatggc acctatttgt atgattatca tgcaaaaacc gtgctggaag
     2461 cggacccaat gcccgttaat aaagccctgc aactcattga gatgctgaat gaacaccaca
     2521 ttcacggtct gatgtatgtg gatgatgcaa tggtctatga gcacccgacc gggcatgtca
     2581 ttcgcacgtc taactgggcg caaaccctgc cgccggaaca gcgtccgact ttcacacaag
     2641 tcgcttctct ggctgaaacg gcgcaacaag ttaacgccgt atggaagttc gccctgacgc
     2701 acgatgacct gccgcaattg cagcattttg gtaagcatgt cgaacatgaa ctgggactgg
     2761 agtgtgaatg gtcctggcac gatcaggttg atattgcacg cggcggcaac agcaaaggta
     2821 aacgtttgac gaaatgggtt gaggcgcaag gttggtcgat ggaaaacgtc gtggcattcg
     2881 gcgataactt taatgatatc agtatgctgg aagccgctgg tacaggcgtg gcgatgggca
     2941 acgccgatga cgcggtaaaa gcgcgcgcca acattgtgat tggtgataac accaccgaca
     3001 gcattgccca gttcatttat agccacctga tttaatcagg cggttatcga cacactttta
     3061 atttgcgcgt acagccacag gccaggtttg atcgccagtt catccctggc ccacgggctg
     3121 atacgcgccc acagcgtttt accgccgact tccagttcca cttccacctg gccgttgtcg
     3181 tcataactat taacaacttt tgcccgcaat acgttacgaa tgctggtttg ctgcggcggt
     3241 tgtaaaacca gagaaacatc ggaagcctga atgcggatac gtagcgcagc ttgcagcggt
     3301 tcgtccagct tattgaccca caaatgctga tcgcccagcg ccagcgcggt catcgcgtaa
     3361 tgcggatgat gctcaagcac cgtcactttc agaatgctac tttgttgctc tttcggcagc
     3421 cacggattca tcacgctact gccccacact tcctccagcg cgccaaaggc tttcacctga
     3481 ccgttttcca gtaccatcac tctgtctgcc agatggagga tctcatccag cgaatggctg
     3541 acatacaaca tcggaatgtt gatttcccgt gtcagccgtt gcagataagg caacagttcg
     3601 cgtttacgcg gaatatccag tgacgccagc ggttcatcca gcaacagcaa ttccggtgct
     3661 gtcagcaaag cccgaccaat cgccacgcgc tgtttttcgc ctccggacag gctgcctggt
     3721 aaacggtcaa gcaacggttc aatgcctaaa agcgccacca gcttatcgaa ctgatcgacc
     3781 atacttttcg acatgccgta gcgcagattg ccacgcactt tgtaatgcgg gaacagccgc
     3841 gcatcctgaa aaacatagcc aacgcgacgc ttttccggcg tcaggcagat acctttttcg
     3901 gcatcattta gtacccgccc attgaggaca atccgccctt tttgcgggcg cgtcagtcca
     3961 ctgatggcgt taatcagcga agtttttccg gcaccggaga cgccaaagat agcagtgatg
     4021 ccattggcgg gcagcgtttc attaatagtc aggcaatggt tgcccaacgt ctgggaaaaa
     4081 ttcagttcca gcatgattag cgccccgccc gttcacggct gattctggcc agccattctg
     4141 aaatcaacag ggagatcatc gccagcgcaa tagaaataat gcacagtctc gccgctccac
     4201 tttccccgcc gggggtctgg atcagggtat acatggcaga aggaatggtt cgcgtttcac
     4261 caggaatgtt cgacacaaag gtgatggttg caccaaactc accgagagaa cgagcaaaag
     4321 ccagtaccgt accaacaata attcccggta aggtcagcgg taacgtgata gtaaagaaaa
     4381 cgcgccagcg cccggccccc agtgttcttg cggcctgttc cagtttgaca tcaacccctt
     4441 ccagcgccag acgaattgcc cgcaccatca gcggaaacga catgacggcg gcagcgagaa
     4501 ccgcgccgcg ccagctaaag gcgaaggtaa taccaaacca gtcatacaga cgttcaccga
     4561 taaatccgcg ccgtcccatc gaaactaata ataagtaacc gacgaccacg ggcggtaaca
     4621 ccagcggtag atgcagtacg ctgtcgagca gagctttgcc cggaaacgtg caacgcacca
     4681 gtaaccaggc aaaaaagatc ccaaacggca ggctaaacag cacagccagg gaagaaactt
     4741 tcaggcttaa taaaactgcc tgccattctg gatcggtcag tatcattact tggttgtaaa
     4801 tccgtaacgt ttaaagattt ccgcagcctg cggtcccttc agataatcat aaaatgcttt
     4861 cactgtcgca ttgttatgcc cttccacaac agcaaccgga tattccactt ttttatgtga
     4921 atcttccggg aagatggcaa ccacttttac ccctttgctg gcaactgcgt cagaaccgta
     4981 aacaatgccc aacggcgctt cattgcgttc gaccaacgcc agcgcgccac gaacatcttc
     5041 cgccggagcc agtttcgggg agagcgtatc ccatgcgccc agtttttgca gtgcttcttt
     5101 tgcataaatg ccagcaggga catgttccgg atcgccaacc gcgaggcgac cgccattcag
     5161 cagcgaagtc cagttagttt tgctgtcgat ggtgaaatct ttctgctcgc tggctttcgg
     5221 tgctacaacg accaggctat tgccgagcag tgtctgacgc gtagcagtat cgatcgcttt
     5281 tttatcaacc gcataatcca tccatttctg atcggcagaa ataaacagat ccgcaggggc
     5341 acccgcttca atctgacggg cgagagtaga tgacgaagcg aaagaagaaa ccacatccac
     5401 gcctttctct tttttatact gcgtagcaat gttctgcatt gcgttagtca gtgatgcggc
     5461 ggcgaacacc gtgattttcc cttcatctgc cagtgcattg ccagcaacag cgaaagagag
     5521 tgccgccccg gcaaacaagt tcaaccattt acgagccatt ggtaacccct taatgtaacg
     5581 ttatgtaggc gacaatataa cgactaactc ggaattttcc cagcagttat tgctaacctt
     5641 tgtgaggtag ataagaaaaa atatcggcag gaaaagcagg aagttgagaa aaagaaaatg
     5701 cccgactaag cgggcattca gggaatcaat gattttgtcc gggctggtct tttttaccaa
     5761 caccagaaaa gatgttgaat acttcaccaa gaccgtaaat cagacccagg atgatggcca
     5821 tcacgacagg taccataatt acggcgaata ccagactttt taataactct aacatggtca
     5881 actccagata tagtcatgag actattctaa ccgctaagca cagaaaagca ctcccctttt
     5941 gtgcggtcag ctttgcgtgg cgttcgtttt ccgtcacaat aagacttttg ccaggacatt
     6001 gttatgcagg ccgaaatcct tctcaccctt aagctccaac aaaaattatt cgccgacccg
     6061 cgccgcattt cgctactaaa acacattgcg ctttccggtt ccattagcca gggagcgaaa
     6121 gatgccggta tcagctataa aagcgcctgg gatgccatta acgagatgaa tcagttaagt
     6181 gagcatattc tggtcgagcg cgcaacaggc ggtaaaggtg gcggcggcgc agtactgacc
     6241 cgctatggtc agcgactgat tcagctctat gacttactgg cgcaaatcca gcaaaaagcc
     6301 tttgatgtgt taagtgacga tgacgccctg ccgctgaaca gcctgttggc cgcgatctca
     6361 cgtttttcac tgcaaaccag cgcccgtaac cagtggttcg gtaccatcac cgcccgcgat
     6421 catgatgacg ttcaacagca tgttgatgtc ttactggctg acggaaaaac acgcctgaaa
     6481 gtcgcaatta ccgcacaaag cggcgcgcgt ctggggctgg atgaaggcaa agaagtgttg
     6541 atattgctaa aagcgccgtg ggtaggcatt actcaggacg aagcggtcgc gcaaaacgct
     6601 gacaaccaat taccgggtat tattagccat attgagcgcg gcgcagagca gtgcgaagta
     6661 ataatgtcgc taccggacgg gcaaacactg tgcgccacag tgccggtaaa tgaagcgact
     6721 tctcttcagc aaggacagaa tgtcacggcc tactttaatg ccgacagcgt gattatcgcc
     6781 acgctgtgct aagcgtgttg acaatttgtt atgaaacacg tatccctgtc agtaatcgct
     6841 gcacaaagtg ggatataaaa tgtcatcgtt gcaaattttg caaggcgcgt ttcgtcttag
     6901 cgacacaaaa acgctgcaat tgcctcagct aacgttaaac gcgggtgata gttgggcgtt
     6961 tgtcggttcg aatggaagcg ggaaatcggc gctggcccgc gcgctggcgg gggaacttcc
     7021 gcttttgaaa ggtgaacggc aaagccagtt ttcccacatc actcgtctct ccttcgagca
     7081 attgcaaaaa ctcgtcagcg acgaatggca gcggaataac accgatatgc tcggccctgg
     7141 cgaagatgac accggacgca ctacggctga gatcattcag gatgaagtaa aggatgcacc
     7201 gcgttgcatg caactggcgc agcagttcgg tattaccgcc ctcctcgacc gacgctttaa
     7261 atacctttcc actggcgaga cgcgaaaaac cctgctgtgt caggcgctga tgtcggagcc
     7321 tgacttgttg attcttgatg agccgttcga tggcctggat gttgcctcac gtcagcagct
     7381 ggctgagcga ctcgcctcgt tacatcagtc cggtattact ctggtactgg tgctcaatcg
     7441 cttcgatgag atcccggagt ttgtccagtt tgctggcgtg ctggcggatt gcacgttagc
     7501 ggaaactggc gctaaagagg aactgctcca acaagcactc gtcgcgcaac tggcgcatag
     7561 tgaacagctt gaaggtgtgc aactgccgga gccggatgaa ccttcagcac gtcacgcctt
     7621 acccgccaac gaaccgcgca ttgtgctgaa caatggcgtg gtttcttata acgatcgccc
     7681 cattcttaat aaccttagct ggcaggtgaa tccaggcgaa cactggcaaa ttgtcgggcc
     7741 aaatggtgca ggaaaatcga cgttattaag cctggttact ggcgatcatc cgcaaggtta
     7801 cagcaacgat ttgacgcttt tcggacgacg tcgcggcagc ggcgaaacca tctgggatat
     7861 caaaaagcat atcggttacg tcagcagtag tttgcatctg gattaccggg tcagtactac
     7921 tgtgcgtaat gtgattcttt ctggctattt tgattcgatt ggcatttatc aggccgtttc
     7981 ggatcgccag caaaaactgg tgcagcagtg gctggatatt ctcggcattg ataaacgtac
     8041 ggctgacgct ccgttccata gtctttcctg gggacagcag cgtctggcgc tgatcgtccg
     8101 cgcactggtg aaacatccga cgttgcttat tctcgatgaa ccactacagg ggcttgatcc
     8161 gctcaatcgc cagcttatcc gccgttttgt tgatgtgctg attagcgaag gtgaaacgca
     8221 attgttgttt gtttcgcacc acgctgaaga tgcgcctgcc tgtattaccc atcgccttga
     8281 gttcgtgccg gacggtggac tctatcgcta tgtgctgaca aaaatatatt gagtcggtag
     8341 tgctgacctt gccggaggcg gcctaagcgc cctctccggc caacggttcg acgcatgcag
     8401 gcatgaaacc gcttcttttt tcagataaaa agcgcaatca ttcataaacc ctctgtttta
     8461 taatcactta atcgcgcata aaaaacggct aaattcttgt gtaaacgatt ccactaattt
     8521 attccatgtc acacttttcg catctttgtt atgctatggt tatttcatac cataagccta
     8581 atggagcgaa ttatgagagt tctggttacc ggtggtagcg gttacattgg aagtcatacc
     8641 tgtgtgcaat tactgcaaaa cggtcatgat gtcatcattc ttgataacct ctgtaacagt
     8701 aagcgcagcg tactgcctgt tatcgagcgt ttaggcggca aacatccaac gtttgttgaa
     8761 ggcgatattc gtaacgaagc gttgatgacc gagatactgc acgatcacgc tatcgacacc
     8821 gtgatccact tcgccgggct gaaagccgtt ggcgaatcgg tacaaaaacc gctggaatat
     8881 tacgacaaca atgtcaacgg cactctgcgc ctgattagcg ccatgcgcgc cgctaacgtc
     8941 aaaaacttta tttttagctc ctccgccacc gtttatggcg atcagcccaa aattccatac
     9001 gttgaaagct tcccgaccgg cacaccacaa agcccttacg gcaaaagcaa gctgatggtg
     9061 gaacagatcc tcaccgatct gcaaaaagcc cagccggact ggagcattgc cctgctgcgc
     9121 tacttcaacc cggttggcgc gcatccgtcg ggcgatatgg gcgaagatcc gcaaggcatt
     9181 ccgaataacc tgatgccata catcgcccag gttgctgtag gccgtcgcga ctcgctggcg
     9241 atttttggta acgattatcc gaccgaagat ggtactggcg tacgcgatta catccacgta
     9301 atggatctgg cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa gccaggcgta
     9361 cacatctaca acctcggcgc tggcgtaggc aacagcgtgc tggacgtggt taatgccttc
     9421 agcaaagcct gcggcaaacc ggttaattat cattttgcac cgcgtcgcga gggcgacctt
     9481 ccggcctact gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg cgtaacgcgc
     9541 acactcgatg aaatggcgca ggacacctgg cactggcagt cacgccatcc acagggatat
     9601 cccgattaag gaacgaccat gacgcaattt aatcccgttg atcatccaca tcgccgctac
     9661 aacccgctca ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg cccctggcag
     9721 ggggcgcagg aaacgccagc caaacaggtg ttacctgcgc acgatccaga ttgcttcctc
     9781 tgcgcaggta atgtgcgggt gacaggcgat aaaaaccccg attacaccgg gacttacgtt
     9841 ttcactaatg actttgcggc cttgatgtct gacacgccag atgcgccaga aagcaacgat
     9901 cctttgatgc gttgccagag cgcgcgcggt accagccggg tgatctgctt ttcaccggat
     9961 cacagtaaaa cgttgccaga actgagcgtt gcggcattga cggaaatcgt caaaacctgg
    10021 caggagcaaa ccgcagagct gggaaaaacg tacccgtggg tgcaggtctt tgaaaacaaa
    10081 ggtgcggcga tgggctgctc taacccgcat ccgcacggac agatttgggc aaatagcttc
    10141 ctgcctaacg aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc cgaacagaaa
    10201 tcaccaatgc tggtggatta tgttcagcgc gagctggcag acggtagccg taccgttgtc
    10261 gaaaccgaac actggttagc cgttgtgcct tactgggctg cctggccgtt cgaaacgcta
    10321 ctgctgccca aagcccacgt tttgcggatc accgatttga ccgacgccca gcgcagcgat
    10381 ttggcgctgg cgttgaaaaa gctgaccagt cgttatgaca acctcttcca gtgctccttc
    10441 ccctactcta tgggctggca cggcgcacca tttaatggcg aagagaatca acactggcag
    10501 ctgcacgcgc acttttatcc gcctctgttg cgctccgcca ccgtacgtaa atttatggtt
    10561 ggttatgaaa tgctggcaga gacccagcga gacctgaccg cagaacaggc agcagagcgt
    10621 ttgcgcgcag tcagcgatat ccattttcgc gaatccggag tgtaagaaat gagtctgaaa
    10681 gaaaaaacac aatctctgtt tgccaacgca tttggctacc ctgccactca caccattcag
    10741 gcgcctggcc gcgtgaattt gattggtgaa cacaccgact acaacgacgg tttcgttctg
    10801 ccctgcgcga ttgattatca aaccgtgatc agttgtgcac cacgcgatga ccgtaaagtt
    10861 cgcgtgatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcga tgcgcccatt
    10921 gtcgcacatg aaaactatca atgggctaac tacgttcgtg gcgtggtgaa acatctgcaa
    10981 ctgcgtaaca acagcttcgg cggcgtggac atggtgatca gcggcaatgt gccgcagggt
    11041 gccgggttaa gttcttccgc ctcactcgaa gtggcggtcg gaaccgtatt gcagcagctt
    11101 tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc agaaaaccag
    11161 tttgttggct gtaactgcgg gatcatggat cagctaattt ccgcgctcgg caagaaagat
    11221 catgccttgc tgattgactg ccgttcactg gggaccaaag cagtttccat gccgaaaggt
    11281 gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc tggttggcag cgaatacaac
    11341 acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc tctgcgcgat
    11401 gtcaccattg aagagttcaa cgctgttgca catgagctgg acccaatcgt ggcgaaacgc
    11461 gtgcgtcata tcctgactga aaacgcccgc accgttgaag ctgccagcgc gctggagcaa
    11521 ggcgacctga aacgtatggg cgagttgatg gcggaatctc atgcctctat gcgcgatgat
    11581 ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc tgtgattggc
    11641 gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt cgcgctgatc
    11701 ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca
    11761 ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg
    11821 aaactcccgc actggcaccc gatggtcagc cgtaccgact gttaactttg cgtaacaacg
    11881 cagggatggt agtcacgctg atggactggg gtgcgacttt actttcagct cgtattccgc
    11941 tttccgatgg cagcgtccgc gaggcgctgc tcggctgtgc cagcccggaa tgctatcagg
    12001 atcaggctgc gtttctgggg gcctctattg gtcgttatgc caaccgcatc gccaatagcc
    12061 gttatacctt tgacggtgaa accgtgacgc tttcgccaag tcagggcgtt aaccagctgc
    12121 acggcggccc ggaagggttc gacaaacgtc gctggcagat tgtgaaccag aacgatcgtc
    12181 aggtgctgtt tgccctgagt tcagatgatg gcgatcaggg cttcccgggt aatctcggcg
    12241 cgacggtgca atatcgtctg accgacgata accgtatctc tattacttat cgcgccacag
    12301 ttgataaacc ttgcccggtg aatatgacta atcacgtcta tttcaatctt gacggcgagc
    12361 agtctgacgt gcgcaatcac aagttgcaga ttctggcgga cgaatatctg ccggttgatg
    12421 aaggcggcat tccgcacgac ggcctgaaat ctgtcgccgg aacgtctttt gatttccgta
    12481 gcgccaaaat catcgccagt gagtttcttg ccgacgacga tcagcgcaaa gtgaaaggtt
    12541 acgatcacgc attcttgtta caggccaaag gcgatggcaa gaaagtggcg gcgcatgtct
    12601 ggtcagcaga tgaaaaattg cagctgaagg tctacaccac cgctccggct ctgcaattct
    12661 actccggcaa cttcctcggc ggcacgccat caagaggaac tgaaccttac gccgactggc
    12721 aaggcctggc actggaaagc gagtttctgc cggacagccc aaaccaccct gaatggccgc
    12781 aaccggactg cttcctgcgt cctggcgaag agtattccag cctgacggaa tatcagttta
    12841 ttgctgagta gcacctgaag ccctcgtaat gagggcttta tccttatatt gctcaaatca
    12901 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgacacta
    12961 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    13021 tattatcatt aaaaaatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    13081 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    13141 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    13201 gttacagctt tgactttgct tacacctctg tgctgaaacg cgctatccat accctgtgga
    13261 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    13321 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    13381 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    13441 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgaa aaagaactgc
    13501 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    13561 ttctgccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    13621 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag cttaatatcc
    13681 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    13741 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag
    13801 cgaagtaaac gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg
    13861 ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc gacttatcag gcctgtggtg
    13921 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    13981 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc caatgcaggc
    14041 atcggtgatg ctcttaccgt aggccagcgg ctccccgcta tcgaggctct gattgccttc
    14101 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    14161 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    14221 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    14281 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcacc
    14341 gttaccgctg gtattcacaa tcgccgaatg cccccattta gttacggaca ggaagcagtg
    14401 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt ctgtgccatt
    14461 tttaaaacct accggacaag aaagaccaga cgccagttcg cggtgcacct gcgattcggt
    14521 ggtacgtgca ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat
    14581 atcgagaaac tcacctgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    14641 ggctatacgc agaccgtcgt tgatctggaa gctattatcc atatgcggat cgttaatcag
    14701 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    14761 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt ttgctgcgac
    14821 aggatcatga attgagcatg ggccaatcac caccaacagg cgatcatcat tacctttcag
    14881 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg
    14941 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tacgtaaatc
    15001 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    15061 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacactat gaacggatta
    15121 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    15181 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataac
    15241 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    15301 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    15361 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    15421 caaactaata acggccaggc aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc
    15481 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggtggc
    15541 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    15601 cacaagaaca ccatgtgcaa agatggtcgc tgtccggata tcaataaaaa ggtacaaacc
    15661 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    15721 aaaatagcca ataatggaga gcggagtctc tcccttttaa tttttcactt ctaaaagcag
    15781 tatgatgtca taaagttaag tgagaataat taagggatga ctcgtcatgg cgcactcaca
    15841 ctcacacacg tcctcacacc tgccagaaga taataatgct cgtcgcttgt tgtatgcttt
    15901 cggcgtgact gccgggttta tgctggtaga agtcgttggt ggttttcttt ctggttctct
    15961 ggcattgctg gccgatgcgg gtcatatgtt gaccgatact gccgccctgc ttttcgccct
    16021 actcgccgtg caattttcac gtcgtcctcc caccattcgc cacactttcg gttggctaag
    16081 actgaccacg ctcgcggcgt ttgtgaatgc catagccctc gtggtaatta ccattttgat
    16141 tgtctgggag gcgatagaac gcttccgcac gccgcgtccg gtcgagggcg gcatgatgat
    16201 ggcaattgcc gtggctgggt tgctggcaaa tatactttct ttctggttac ttcatcacgg
    16261 cagcgaagag aaaaacctca acgtacgagc agcggcactg catgtgctcg gggacctgct
    16321 gggttcggtt ggggctatta ttgccgcgtt gattattatc tggactggct ggacacctgc
    16381 tgaccccatt ctctcgatac tggtgtcgct tctggttctg cgtagcgcgt ggcgactgtt
    16441 gaaagatagc gtgaatgaat tacttgaagg tgcaccggta tcgctggata tcgctgaact
    16501 gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat gtgcaccatg tacatgtatg
    16561 gatggtaggc gagaagccgg tgatgacgtt gcatgtgcag gtgatcccgc cacacgatca
    16621 cgatgccttg ttggatcaga tccaacacta cctgatggat cactatcaga ttgagcacgc
    16681 caccattcag atggaatatc aaccttgtca tgggccagac tgccacctga atgaaggggt
    16741 gtctggccat tcacatcatc accattaatg ggacagcgcg cgtgagcctc tttcacgtgc
    16801 gctgttgatc cacatccggc tgccgttgag cgcaataaag gtcaggatga tgtactccag
    16861 agacatggcg taaacgccct gaagtgcaaa aataacgacg ctaatcacgt taataatcac
    16921 ccacaacagc cagttttcca catacttacg cgtcatcaga atcattgcca cgatagataa
    16981 caccatcatg catgaatccc agaacgggaa agcgtccggt tgcagttcag gcatcacaac
    17041 ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg gtcaaaaatg caaacaccgg
    17101 attgataaat accgtcatca gaccaatcga aacaacgcaa accgccaacc agctgagtgc
    17161 cttcggcaat ggcaaccagc gaattttcaa ctccgcctcg ttctgactgg tttgtcgcga
    17221 ccacgcatac caaccgtaaa tattcgcggc aaagaaaaac acctgtaata gcaggctggc
    17281 atacagctga atctgaaaga aaataatgcc aaacaaggtg acgttgatca ggccaaagaa
    17341 gtagttgctg atcttctcca gactggcaag gccaatacac agcaacccgg cgatcgtgcc
    17401 taccgcttcg atccatgaga gatcataacc gcctgcccct attggtatat gtaccaggat
    17461 attctgcaca ctaaaaaaat ccatctttcc cccaaagcgc cttattattc gttatccacg
    17521 tagtgtagcc gcaaaatcca gcatacgatt gagcggcacc agcgccctct ctcgcagcct
    17581 ttcatcaaca tgaacctcgt gattgcttcc ttcctgttct aatgcctctg cgatggactg
    17641 aaggccattc atggccatcc acggacaatg cgcgcagctg cggcaggttg caccctcacc
    17701 tgcggttggt gcttccagta actctttatc tggcactgcc tgctgcattt tgtagaaaat
    17761 gccccgatcg gttgccacga taagccgctg atgtggcaat gctttcgcag cagcgatcag
    17821 ttgactggtg gaaccgaccg catccgccat atcgacaata gcttgtggtg attctggatg
    17881 caccagtatg gcagcatccg ggtattcttc ttgcaagcgg gttaacgcct gagtcttaaa
    17941 ttcatcatgc acaatacagg caccctgcca gcatagaatg tctccacccg tctgtttttg
    18001 cacgtaacgc cccagatgtt tgtcgggtgc ccagatgatt ttttcaccca aactatcaag
    18061 atgatcaata agttcgacgg aaatgcttga agttaccacc caatctgcgc gcgcttttac
    18121 cgcagcagaa gtgttggcgt agacgacgac agtacgatcg ggatgggcat cgcaaaatgc
    18181 gttaaattct tcaacagggc agccgagatc cagtgaacat tcagcctgaa gtgtcggcat
    18241 cagaattgtt ttttccggac tgagaatttt ggcggtttct cccataaatc tcaccccagc
    18301 gactaacaaa gtagaagcgg gatgctttgc accgaagcgc gccatttcca gagaatcaga
    18361 aatacagcca ccggtttctt ctgccagttg ttgaatttcg ggatcggtat agtagtgggc
    18421 aaccatcacc gcattacgtt cttttagcag acgttttatc ttctcgcggt aatacgcttt
    18481 ttcatcaatg cttaacggcg tcggcttcgg ggggaaagga taaatcgccg tgtctggatc
    18541 aaacattacg ctcatcttac catctcgttt tacaggctta acgttaaaac cgacattagc
    18601 gtaatattcg ctgttatcgg tgtaatttgt tttatatgct aaacaagata gtcgaatggt
    18661 ggggaaaagt cactcgaatt acgcctaatt ccaccgacgt agagttgctt gcacagtaag
    18721 ctctgacgat gtcatcaggt ttacaaagtg aaattgagga tgcctgaata gcaaaaaagc
    18781 gcctttaggg cgctttttta cattggtggg tcgtgcagga tgactcgctt cgctcgccct
    18841 tcgggccgtc gccgcgagcg gctccgttgt ttcgctgcgc tcaactcaaa cctgctgcag
    18901 gttcgaatct ttgagtatcg cagatagcaa aaaagcgcct ttagggcgct tttttacatt
    18961 ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact gctctaccaa
    19021 ctgagctaac gaccccttgc gggattatgc tgctttaatc attcaggatg gtgggtcgtg
    19081 caggattcga acctgcgacc aattgattaa aagtcaactg ctctaccaac tgagctaacg
    19141 acccatacgg gtgttacctg aaagatttct actcggggcc aatctttaag attggtgtgt
    19201 cgtgcaggat gactcgcttc gcttgcccta cgggccgttg ctggcgcaac gttatccttc
    19261 acgcttaaca tctgagtttg atgttaaaat tggtgggtcg tgcaggattc gaacctgcga
    19321 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccgagt ggtgggtgat
    19381 gacgggatcg aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat
    19441 cacccgatac tgcgctggat actacaaact acttccaccg aacccaccgt aattagtggt
    19501 gggtcgtgca ggatgactcg cttcgctcgc cctacgggcc gttgctgacg caacgttatc
    19561 ctacacgctt aacatctgtc ttcgatgcta aaactggtgg gtcgtgcagg attcgaacct
    19621 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc ggtggtgggt
    19681 gatgacggga tcgaaccgcc gaccccctcc ttgtaaggga ggtgctctcc cagctgagct
    19741 aatcacccaa tactacgctg gatactacaa ctaactatgt tagtggtggg tcgtgcagga
    19801 tgactcgact tcgtctcgcc cttcgggccg ttgctgatgc aacgttatcc ttcacgtttt
    19861 actaccgctt ccaccttagc gattggtggg tcgtgcagga ttcgaacctg cgaccaattg
    19921 attaaaagtc aactgctcta ccaactgagc taacgaccca ctttttcgtt gctttcggtt
    19981 tgtttgatat cccgtggcaa cggcggcata tattactgat ttcagacttg agcgcaacaa
    20041 aaatttcgat gcagatcact caactgctta tgattcgcac gacacgacca gaaataatgc
    20101 gacttctggt cgtgtgttat gcattacatc gcgttcagac gtttttgtgc ctgtttagcg
    20161 ccatcagtac cagggtattt actgataacc tgctggtata cggctttcgc ttttgcggtg
    20221 tcacctttgt cctgcatgat gacgccgact ttaaacatcg catcagcagc ctttggtgac
    20281 ttcggatagt ttttcactac cgaagcaaaa tagtacgccg catcatcttt tttacccttg
    20341 ttgtagttta actgacccag ccaataattg gcgtttggca ggtaagttga atcagggtaa
    20401 tttttgatga aattctgaaa tgccaccatt gcgtcatcct ggcgggattt atcctgcacc
    20461 agcgcaatag ctgcattgta atccgtgttt gcatcgccgc ttttcaccgg cgcgccagca
    20521 ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg ccgcaccgct ttgatcgctg
    20581 ctggttgatt gcgccgctgc accaccgctg ctgaggctgt cgatctgcaa caggatctgc
    20641 ttttgccgct ccacgacctg attcagttga tactgatttt cctgaatctg accacgcagg
    20701 gaatcaatat cggattgatt atcagaaagt tgttgctgga gttgggttaa aagctggctg
    20761 tgagcattag aaatacgctc aagttgagtg acgcggtctt cgaccgagcc tgagccgaca
    20821 ctactgattg gtgcctgagc aaaagcggcc cagggggccg ctataccaac cagtaacgac
    20881 agactcaata gttgatgtct gaagttactg ctcatgcaat tctcttagta aaccagtacc
    20941 gcacgacggt ttttggagta tgccgcttcg tcatgaccca gtactgcagg tttttcttta
    21001 ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt taccctgcag gtacatctta
    21061 acggcgttcg cacgacgttc acccagggag atgttgtatt ccggagtacc acgttcgtcc
    21121 gcgtgacctt ctacggtgac tttgtaagac gggttgctac gcaggaagtt tgcatgtgca
    21181 tccagcattt gagcgaagtc agaacggata tcgtacttgt ccagatcgaa gtaaacgatg
    21241 ttgttctgct gcagctgttg catttgcaga cgagcctgct cttcggaaga catgttgccg
    21301 ttgccgccgt tcgcatccat accagtgccg gcacccagca tgccttcgct gccgtcattg
    21361 ctggcgttct tgttggaaga acatgccgca attgccataa caggcagagc aatcatcagc
    21421 cctttcagca ctttgttcag ttgcatttca atgattcctt tactattcaa ttaattatta
    21481 tcacagatac ggcgaccagg cagggaattt gacctgtcca tcagttgccg gaagacgcgc
    21541 tttgaaacgc ccatctgtag aaaccaaatt cagcacggat cccatcccct gagaagagct
    21601 gtagattacc atagtgccgt taggtgccag acttggcgtt tcatccagga acgtggacga
    21661 cagaacttgt acgcctcccg ttgccagatc ttgtttggca atgtgctgct gcccaccatt
    21721 ggagctgacc attaccataa atttaccgtc gctgctgaca tccgcatcct ggttctgcga
    21781 accttcccag gtaatacgtt gtggcgcacc gccgttgata ttcactttat aaacctgcgg
    21841 acgaccggcc tggtcagaag taaatgccag gttctggcta tccgggaacc aggtcggttc
    21901 ggtattgtta ctgcgaccat cagtcacctg gcggatctga ccagaagcca aatccattac
    21961 gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc agtttgctgc cgtctggcga
    22021 gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc tgacgtacag cgccatttgc
    22081 cagcgtctga ataaccagcg cggaacgacc gctttcgaag gtcacataag ccagtttaga
    22141 accgtctggt gaccacgccg gagacatcag cggctgcggt gaacggtgaa cgacaaactg
    22201 gttgtaaccg tcatagtcag atacgcgcag ttcatacggg aactgaccgc cgttggtctg
    22261 aacaacgtag gcaatacgag tacggaacgc acctttaatg ccggtcagtt tttcaaacac
    22321 ttcatcactg gcggtatgac cagcataacg cagccactgc ttgttcactt tgtacgagtt
    22381 ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca agttgataag caacattgta
    22441 agaaccatcc ggattcggag tgacctgacc gacaactaca gcgtcaatgc ccagtgcgga
    22501 ccatgcagct ggttgtactt cctgcgcact acccggctgc tgtggcagac gagcgcgatc
    22561 taacggatta aatttaccgc tgttacgcaa gtcagcagca acgatgccgc caatatcttc
    22621 aggtgccgca ccaggccccg cccactggaa aggaacaaca ccaataggac gaccggaatc
    22681 tacaccgctg tcgatcacaa tgcggacttc agcatgcaga actgatgccc acagtatgag
    22741 aaaaccaaat gctactcgta atgcctgctt catcatatct cccttatctg gaccgatggc
    22801 ccacgataat ttagcagaat gttaacaaac taaaatacac aaaactacca gaaccccgtg
    22861 gcaaccggtg cctgatgttg accgtccgaa cagtcaacat cgcgattacg gtttgaagtc
    22921 caatggcgcg tttttgaaca cttcatatac tgcctggctt ggtggtttcg ggatcttcgc
    22981 aagtttagct gctgccaacg cagcctgaca aagtgcggga tcgccacctt caggtttgat
    23041 atccagtaac ataccatcgg gtgccagttt tatgcgcagc gtacaggttt tgcctgcata
    23101 ggacgatgcg tcatagaact tactttcgat agcagattta atctgcccgg catagttatt
    23161 gatatcggcc cctgatgcgc cattgttttt agtattacca ctcccggcag gcgaagcatt
    23221 gttccctttc gcccctcccc ccgttttcgg tgcattctta ccagagctta gctcaccgaa
    23281 aatatcatct gcctctgcgg ccgcttttgc tgcagcggct ttttcagctg cagcttttgc
    23341 cgctgccgct tttttgtctg cggcggcttt ttctgccgct gctttcttat cagctgctgc
    23401 cttttcagca gccgcttttt ctgctgcttt tttgtcggct gcagctttct ctgctgccgc
    23461 tttcttatca gctgcagcct tttcagcagc cgctttcttc tcagcttctg ctttggcttt
    23521 ttcagcagct tcagttgccg ctttctttct tgcttcagct gcagctgctt ctgccgcttc
    23581 cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctt cggctgcggc
    23641 tttggcggct tctgcttctg cttttttctt tgcgtctgca gccgctttct tcgctgcttc
    23701 ttccgcagct ttagcatctg cttcggcctt cgctttagca tctgccgccg ctttcgctgc
    23761 cgcctcttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
    23821 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
    23881 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
    23941 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
    24001 tgaatcaacc atgacagcgt cgatagacga accaccgccg cctccggctg aagcttctat
    24061 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
    24121 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
    24181 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
    24241 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
    24301 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
    24361 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
    24421 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
    24481 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
    24541 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
    24601 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
    24661 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
    24721 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgaggc
    24781 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgcagtc gcaatcaacg
    24841 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
    24901 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
    24961 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
    25021 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
    25081 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
    25141 cccgtttccc ctggctctct tgatagaggc gagagagttc gattccagac cagaatttat
    25201 cttcaaacgc ttcggcttcg cgcgccgctg cgttaagaat acgggtccgc tggataataa
    25261 tggcccaaga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
    25321 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
    25381 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
    25441 tgcttcattc agcaaagtat tctcggcgtt gacaatacgt tgcgtgaaaa ccaaagaggt
    25501 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
    25561 gtaatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
    25621 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
    25681 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
    25741 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
    25801 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
    25861 aaaatttatg ggcctggagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
    25921 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
    25981 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
    26041 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
    26101 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
    26161 cgcttgtcca ttaccgcata caaagtcgcg ataatcttac tcatcagatg tcttcttgac
    26221 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
    26281 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
    26341 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
    26401 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
    26461 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
    26521 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
    26581 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
    26641 cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
    26701 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
    26761 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
    26821 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
    26881 gtgcagttcg cccacggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
    26941 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
    27001 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
    27061 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
    27121 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
    27181 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
    27241 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
    27301 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
    27361 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
    27421 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
    27481 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
    27541 tttcaggctg cttgggccga ggcgtgcaaa tttgaacatt aagaacaatt ctgccaccag
    27601 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
    27661 tgacgagttc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
    27721 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
    27781 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
    27841 cgcgatgatt gccagaagca ggaagccaca cgccaccatg atacggaacg caaagtacag
    27901 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
    27961 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    28021 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    28081 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    28141 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    28201 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacagagtaa aggcagcagg
    28261 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    28321 catttcgtag ccggattcat cacccaaaac aataacagac agaacagcag ccataccgaa
    28381 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    28441 ccatgcgctg acaccgagga tgaacatcgc gccagtaaca taaccagacg ctacagtgtg
    28501 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    28561 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaac
    28621 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    28681 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    28741 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    28801 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacccc
    28861 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    28921 tttgccggag aggacgtaga ccgtttccat aatggccagc aggaacgcca taccgagcgt
    28981 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    29041 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    29101 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    29161 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    29221 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    29281 aatttgcatt tttcgacggt gatcaattta tagctaaatt accgcctttc agccaatttg
    29341 atcgagaaca atttatctct ttttgatgc
//
LOCUS       DAPHJE010000054        28697 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPHJE010000054 DAPHJE010000000
VERSION     DAPHJE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.054"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..767
                     /locus_tag="RH222_002378"
     CDS             <1..767
                     /locus_tag="RH222_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV3797381.1"
                     /translation="TPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNH
                     ETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYII
                     GPITVSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYR
                     PKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQI
                     NPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1133..2200
                     /locus_tag="RH222_002379"
     CDS             1133..2200
                     /locus_tag="RH222_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3797382.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2197..4932
                     /gene="rbbA"
                     /locus_tag="RH222_002380"
     CDS             2197..4932
                     /gene="rbbA"
                     /locus_tag="RH222_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV3797383.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4932..6056
                     /locus_tag="RH222_002381"
     CDS             4932..6056
                     /locus_tag="RH222_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3797384.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6115..6390
                     /locus_tag="RH222_002382"
                     /pseudo
     CDS             6115..6390
                     /locus_tag="RH222_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6387..6746
                     /locus_tag="RH222_002383"
     CDS             6387..6746
                     /locus_tag="RH222_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV3797385.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6866..7267)
                     /gene="nikR"
                     /locus_tag="RH222_002384"
     CDS             complement(6866..7267)
                     /gene="nikR"
                     /locus_tag="RH222_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV3797386.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7273..8079)
                     /gene="nikE"
                     /locus_tag="RH222_002385"
     CDS             complement(7273..8079)
                     /gene="nikE"
                     /locus_tag="RH222_002385"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV3797387.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8076..8840)
                     /gene="nikD"
                     /locus_tag="RH222_002386"
     CDS             complement(8076..8840)
                     /gene="nikD"
                     /locus_tag="RH222_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV3797388.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8840..9673)
                     /gene="nikC"
                     /locus_tag="RH222_002387"
     CDS             complement(8840..9673)
                     /gene="nikC"
                     /locus_tag="RH222_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV3797389.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9670..10614)
                     /gene="nikB"
                     /locus_tag="RH222_002388"
     CDS             complement(9670..10614)
                     /gene="nikB"
                     /locus_tag="RH222_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV3797390.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10614..12188)
                     /gene="nikA"
                     /locus_tag="RH222_002389"
     CDS             complement(10614..12188)
                     /gene="nikA"
                     /locus_tag="RH222_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV3797391.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12299..12886)
                     /gene="acpT"
                     /locus_tag="RH222_002390"
     CDS             complement(12299..12886)
                     /gene="acpT"
                     /locus_tag="RH222_002390"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV3797392.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12941..13990)
                     /locus_tag="RH222_002391"
     CDS             complement(12941..13990)
                     /locus_tag="RH222_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV3797393.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14122..15339
                     /locus_tag="RH222_002392"
     CDS             14122..15339
                     /locus_tag="RH222_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3797394.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15343..15900)
                     /gene="dcrB"
                     /locus_tag="RH222_002393"
     CDS             complement(15343..15900)
                     /gene="dcrB"
                     /locus_tag="RH222_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV3797395.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15973..16638)
                     /locus_tag="RH222_002394"
     CDS             complement(15973..16638)
                     /locus_tag="RH222_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV3797396.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16859..17104
                     /gene="tusA"
                     /locus_tag="RH222_002395"
     CDS             16859..17104
                     /gene="tusA"
                     /locus_tag="RH222_002395"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV3797397.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17206..19404)
                     /gene="zntA"
                     /locus_tag="RH222_002396"
     CDS             complement(17206..19404)
                     /gene="zntA"
                     /locus_tag="RH222_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV3797398.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGNVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19478..20104)
                     /locus_tag="RH222_002397"
     CDS             complement(19478..20104)
                     /locus_tag="RH222_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV3797399.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20245..20604
                     /locus_tag="RH222_002398"
     CDS             20245..20604
                     /locus_tag="RH222_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV3797400.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20607..20876)
                     /locus_tag="RH222_002399"
     CDS             complement(20607..20876)
                     /locus_tag="RH222_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV3797401.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20866..21462)
                     /gene="rsmD"
                     /locus_tag="RH222_002400"
     CDS             complement(20866..21462)
                     /gene="rsmD"
                     /locus_tag="RH222_002400"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV3797402.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21612..23105
                     /gene="ftsY"
                     /locus_tag="RH222_002401"
     CDS             21612..23105
                     /gene="ftsY"
                     /locus_tag="RH222_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV3797403.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23108..23776
                     /gene="ftsE"
                     /locus_tag="RH222_002402"
     CDS             23108..23776
                     /gene="ftsE"
                     /locus_tag="RH222_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV3797404.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23769..24827
                     /gene="ftsX"
                     /locus_tag="RH222_002403"
     CDS             23769..24827
                     /gene="ftsX"
                     /locus_tag="RH222_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV3797405.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25072..25926
                     /gene="rpoH"
                     /locus_tag="RH222_002404"
     CDS             25072..25926
                     /gene="rpoH"
                     /locus_tag="RH222_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV3797406.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26198..27301
                     /gene="livJ"
                     /locus_tag="RH222_002405"
     CDS             26198..27301
                     /gene="livJ"
                     /locus_tag="RH222_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV3797407.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            27451..27717
                     /locus_tag="RH222_002406"
     CDS             27451..27717
                     /locus_tag="RH222_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV3797408.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            27724..28251
                     /locus_tag="RH222_002407"
     CDS             27724..28251
                     /locus_tag="RH222_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3797409.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(28248..28631)
                     /gene="panM"
                     /locus_tag="RH222_002408"
     CDS             complement(28248..28631)
                     /gene="panM"
                     /locus_tag="RH222_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV3797410.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLCVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6818 a         7426 c         7965 g         6488 t
ORIGIN      
        1 tgacgccatg tacgtttctt ggcgaccaaa ccggcgatcg atttagtaat atctgtggcg
       61 atcaatttat catcgatttg ttaaaacgca taatgagtat caatgaaaat gttcatgtat
      121 tagctggaaa tcatgaaacg aattgcaatg gtaactatat gcagaacttc acgcgtatga
      181 aaccacttga tgaggatacc tacgctggga taaaagatta tcctgtatgt ttctatgacc
      241 ccaaatacaa aataatggca aatcatcatg ggataacttt tgatgagcag cgaaaacgtt
      301 acattatagg accgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      361 aattagcggc aattatcaat aaaaagcatc atgctataat taatggcaaa aagtttaaaa
      421 ctagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      481 gacctaaact tgaagcacta ttagcatgtt cacaaatgtt aggtataaat caagttgttg
      541 cacataacgg aaatggtgga agagaacgca taggcgaaac ggggacagtt ttaggtctta
      601 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      661 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      721 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      781 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      841 ttcctgcaac tgaacaggcg tgaaacgcta tccaacagga tggataccgt tttgtcagcc
      901 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      961 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
     1021 ctggtcgtat ttgccgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
     1081 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggtcga ttatggataa
     1141 gagtaagcgc catctggcgt ggtgggttgt cgggttactg gcggtggcgg ctatcgtggc
     1201 gtggtggctg ttgcgcccgg caggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1261 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtattgaca ccattctggt
     1321 gaaagaaggc cagtttgttc gagaaggtga agtgctggcg aagatggata ctcgcgtgtt
     1381 gcaggaacag cgactggaag ccatcgcgca aatcaaagaa gcacaaagcg ccgttgctgc
     1441 cgcgcaggct ttgctggagc aacgccaaag tgaaactcgc gccgctcagt cgctggttaa
     1501 tcaacggcaa gctgaactgg actccgtagc caaacgtcat acccgttccc gctcgctcgc
     1561 ccatcgtggg gctatttctg cgcaacagtt ggatgacgac cgcgccgccg ctgagagcgc
     1621 ccgagctgcg ctggaatcgg caaaagctca ggtttcggct tctaaagccg ctatagaagc
     1681 ggcacgcacc aatatcattc aggcgcaaac tcgcgtcgaa gccgcacaag ccactgaacg
     1741 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1801 gtatcgggtt gccgagccag gcgaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1861 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctgaa
     1921 actgggcggt gaagcccggc tgatcctcga tgccgcgcca gatctgcgta ttcctgcaac
     1981 catcagtttt gtcgccagtg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     2041 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     2101 tctggaatat gtcaaaaccg gtttaccggg cgtagcgtgg gtgcgggtga atgaagaact
     2161 tccgtggcct gacgacctcg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     2221 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2281 aacaatatca cactcgatat tccggcccgt tgcatggtcg ggctgattgg cccggacggc
     2341 gtcgggaagt cgagcttgtt gtcgttgatt tccggtgccc gcgtcattga acagggcaat
     2401 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2461 atcgcctgga tgccgcaggg gctgggcaaa aatctctacc acaccttatc ggtgtatgaa
     2521 aatgtcgatt ttttcgctcg cttgttcggt cacgataaag cggagcggga agtccgaatt
     2581 aatgagctgc tgaccagcac cgggttagca ccgtttcgcg atcgtccggc aggtaaactc
     2641 tccggcggga tgaagcaaaa actggggctg tgctgcgcgt taatccacga cccggaactg
     2701 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2761 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2821 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2881 actggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaggcgttt
     2941 ataaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     3001 caacctgaaa atgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     3061 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggttttctt
     3121 ggatcgaacg gctgcggtaa atccaccacc atgaaaatgc tcaccggact gctgcccgcc
     3181 agcgaaggtg aggcgtggct gtttgggcaa ccggttgatc caaaagatat cgatacccgc
     3241 cgtcgggtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3301 aaccttgagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3361 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3421 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3481 ttaatcctcg atgaacctac ttctggcgtc gatccggtgg cgagggatat gttctggcag
     3541 ttgatggtcg atctctcgcg ccaggacaaa gtaaccattt ttatctccac ccactttatg
     3601 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gctcgccagc
     3661 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3721 gcctatttgc aggaagcggc aggacagagc aacgaagccg aagcgccgcc cgtgatacac
     3781 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3841 cgcgaagcac tggaactgcg acgcgatccg gtacgttcga cgctggcgct gatgggaaca
     3901 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3961 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacgctcaa tctctccggt
     4021 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     4081 cgtgcgggcg atataacggt ggcaattgag atccctccca atttcgggcg cgatatcgcg
     4141 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgccgag ccgtgccgaa
     4201 acggtaaaag gttacgtgca ggcaatgcac cagagctggc tacaggatgt ggcgagccga
     4261 caatccacac ccgccagcca aagcgggctg atgaatattg agacgcgcta tcgctataac
     4321 ccggacgtaa aaagcctgcc agcgattgtt ccggcggtga tcccgcttct gctgatgatg
     4381 atcccgtcaa tgctaagcgc ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4441 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4501 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4561 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4621 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4681 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4741 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4801 actatcgccc gcggaacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt
     4861 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4921 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4981 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     5041 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     5101 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgcccg tggtttttgc
     5161 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccggg cgttatacct
     5221 ttgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5281 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5341 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5401 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5461 gcggggtgat ggcaatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5521 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt gttggtgatg ccgataacgc
     5581 cgtttgagat catgatggca aaggtctggt cgatggggct ggtggtgctg gtggtgtcgg
     5641 gattatcact ggtgctgatg gtgaaaggcg tgctgggtgt accgattgaa ggctcgatcc
     5701 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5761 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5821 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5881 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5941 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     6001 cgtttttcac catcgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     6061 gggaaacttg acttcatact gtgatgtcat aaagtgaagt cataagggaa tgtcatgtaa
     6121 acacaggtac aggctttacg aaagaaacaa aaaaacacac tggatcagct atttaaatca
     6181 cctttacctc aaggaatcaa atggactgat atagaatctc tggttaaagc gttaggcgga
     6241 gaaattaagg aaggtagggg gtcgcgctgt aagttcatac taaatatgag cgttgcgtgt
     6301 ttccatcggc ctcatccgtc gccagatacc gataaaggcg ctgtagaaag cgtgcgtgac
     6361 tggttactaa gtataggggt aaaaccatga tcaaactgaa gacgccaaac tctatggaga
     6421 ttgccgggca acctgctgtt atcacttacg tgccagaact gaatgcattt cgtggcaaat
     6481 tcctgggctt gtcaggttat tgtgattttg tctctgacag cattcagggg ctgcaaaaag
     6541 aaggggaact ttccctgcga gagtatcttg aggattgtaa agctgcgggt attgagccgt
     6601 atgcgcgtac agaaaagatt aagaccttta cgttacgcta tccagaatcg ctgagtgaac
     6661 gccttaataa tgcggctgcg cagcaacaag tctctgttaa tacctatatt attgaaacac
     6721 tgaatgagcg tttgaatcat ttataaattt tagcggcgta cattacgcag aatatctgcc
     6781 tttacaggat ctatctatgc aactgaactt aattttataa cttaagttca gttaatatct
     6841 tttctttcct cgtttcagaa ataactcaat cttccttcgg caagcactgc aaatgcccgt
     6901 gccgcacgcc gcgctgggcg ataacgtcat cggcaaaatg ctgcacgtcg cccatatcac
     6961 ctttcagcac ggcgatttcc agacagtcgt cgtgattgat atgcacatgc agcgtggcga
     7021 cggagagatc gtggtgatgg tgctgggtgg agacaatgcg gctggctaag tcgcgttttt
     7081 cgtgttcata cacataggag agcaccgcga aaccttgcgt gccgtgctgc tgagtggcct
     7141 cctgggccag ggcgctacgc agaatatcgc ggatagcttc ggaacggttg ttataaccac
     7201 gacgctggct caggctgtcc agcgtctcca gtaaatcgtc atcaagcgtg atggtgactc
     7261 gttgcatttg cgttaaacct tttctgtggt gcgacggcgc acggggaatg cgggtaatac
     7321 cgcgttttgt agcacacgtc cggcgtcaga ggaaaaggtt aatttatctc ccaccacctg
     7381 ggtttcgacg atttgtccgt tgtccatcac catcacccgc tgacagaagc gttccaccag
     7441 gcgtaagtcg tgggtgatga acaggcaggc ggtgccaaac tgttgttgta gctttttcag
     7501 caggcgaatg acgcccgcct gtaacacgag atcaaggtta gaaacggctt catccagaat
     7561 cagtagtttc ggttcgaccg ccagcgcgcg agccaggcag acgcgctgga gctggccgcc
     7621 gcttaactgc ggtgggcgtt tgtcgagaac gctgtcatcg agatcgaccg ccttcagcat
     7681 ttcgctggcg cgcgccagtt gttcggattt tttcagtgat agcaggtggc gcatcggttc
     7741 acgcaggatc tcgcgcacgg ttttgcgcgg attcacggcg ctgatggagt cctgaaatac
     7801 catctgaata tcgcggcgga acgctttacg ctgggcgcga ttgagtttcg ccagcggttc
     7861 gccgcgccag ctaatattcc cttgtgaggg agactctaag cctaccagca gccgcgcgag
     7921 ggtgcttttt ccgcagccgc tgcgtcccag cagggcgacg gtttcgccgc ttttcagggt
     7981 cagggaaacg ttattcagca ccgcctgatg ttgatgtttt ccgctaaatc caccgtgggc
     8041 atagtgatgg gaaaggccgc agacgttaag taaggtcatg atgccagctc cataccgtag
     8101 agggcgagat gagcggaaac caggctgcgc gtcaccgtat gtttgggggc gttaaacagc
     8161 gtttctacat cgccctgttc gacaatttta ccttgtgaca tcaccgccac gtcatccgcc
     8221 agacgcgcca caacgcccat atcatgggtg accagcagca ttcccggcgc ttgtttttgc
     8281 ataatgcttt ccagcagatc gaggatgcgc gcctgtgcta ccacgtcgag gtcggtggtc
     8341 ggttcatcgg cgatgataaa cggtgattca cacagcaccg ccatcgcaat catcatgcgc
     8401 tgcaacatgc cgccgctcat ctcgaacggg tacagcttca gcacgcgcgc ggcgttttcc
     8461 agccccaccg cttctatggc agcggtaagc gtagcgtcat cggcgggttt ccctaacgcc
     8521 aggcaggttt cccgcgcgtg ggtgtgcatg gtgtgcagtg gattaaaggc gctgcgcggg
     8581 ttctgcatga tggtggcaat tttgatgccg cgcagggcgc aaggcgaaac cggtttgcca
     8641 tcggctaaaa tttcccccgc cgtctggcga acgccagcgg gcaaaatgcc cagcgtcgcg
     8701 gcgcaggtta atgatttccc gctgccgcta ccgccgacta acgccagcac gcgcccgcgt
     8761 tgcagggtta acgatacacc gtgtaccagc ggctgcgcgg cctgtagcgc gatattacgt
     8821 agttcaatct gttgcggcat tagtgtgcgt gctccgtcac cagatgagga tccagatgat
     8881 cgcgcagtgc gtcacccacc aggttaaagg ccatcacgct gataaacagc gccagccccg
     8941 gccagaacat ttgcagcggc tgggtccaga tatactggcg cgcgtcgtta atcatcacgc
     9001 cccattcggc ggtcggcgcg gtcacaccga ggccaaggaa agacatcccc gcgacgtgca
     9061 gcatcatatg gccgatatcc agcgttgcca gcaccagcag cgaagggatc accgcgcctg
     9121 ccagatgatc gacaaacacc cgcacatggc ccgcaccgga aagccgtgac gccagcacaa
     9181 actcgcgttg gcgtagtgaa atcaccaggc tgcgcaccat gcgtgcatac cacgcccagt
     9241 gcgacagggc gatggcgata attacgttgg tcagcccggt gccgagcacg ccaaccataa
     9301 agaacgacag aatcgaggtc gggaaggtca taaacatatc ggcgacgcgc atggtggcct
     9361 gatcaacgcg tccgccaatc agtccggcgc tgccgccaat caccagccct aatgtcagca
     9421 ccagcagcag gcaggccatt accgaaccga gcgacacgcg ggtcgccgcc atcagccgcg
     9481 agaaaatatc acgccctaag tgatcggtgc ccagccagtg ctgcgcatcc ggcgaaagca
     9541 ggcgcgacgg cagatcaata gcctgagggt catacggcag ccaccactgg ctggtaagcg
     9601 caatcagcgc cagcagggcg ataatgatca gcgccaggcg taccgaccag cgggaagaga
     9661 ggaaaaagtt cacgcgtgcg ctccttcatg acgacgaatg cgcgggtcca gcgcggcgtt
     9721 gagcaaatcg acaatcaaat tacagaccac aaaaaccacc accatcatca gcgtaaagca
     9781 ctggatcacc ggatagtcac ggttaaaaat cgccgacacc gcatagcgcc cgacgcccgg
     9841 ccaggcaaag atgttttcga taatcatcgt cccgccgatc agttcgccga tgtgcatccc
     9901 cacggcggtg atcatcggca gcgaggcatt gcgcaggatg tggcgacgtt cggtctgttt
     9961 gtcgttcagg ccgcgcagac gcgcccaggt gacgtgacgc tgaccggcga cgtccagcat
    10021 actggcgcgc agtaaacgcg cgttaatcgc cagcgacata aaggcaatgg aaaccgcagg
    10081 caaaatgatg tgctgccagc cgccgtaacc catcgcgggt agccattgca gatacaccga
    10141 aaacgccatc accagtaaaa acgccagcca gaagttaggc atcgacacgc caagaaacgc
    10201 gatgaaacgt acggcgaaat ccggcagacg gtcgcggtgg cgcgccgccc agataccgag
    10261 cggtacggaa gtgagcagaa ttaataccag cgccgcacct gcaagttcca gcgtggcggg
    10321 caggaagttc agcatatcgt ccagtaccgg gcgttggctg gcgaatgaga taccaaagtc
    10381 aagatgcagc gccttccaca accaggtgcc gtactggacg tacagcggct gatccagtcc
    10441 cagcatggtg cgggtagagg ccagcatctc cggcgtcggc ggcaggttag acagacgcaa
    10501 ataatcgagc gccgggtcgc cggtaccgag gcgcagcatc agaaaaatga tcaccgaggc
    10561 ggcgagcacc atcgggatca gcagcagaaa gcggcgtaat acgtaacgca acattaaggt
    10621 ttcaccggtt taatctgttc gaacggaatt tcggtggcga tcggcgcgta ggggatgtta
    10681 cccagctccg gttttgatac caccatcatc gagatgtagc tgataggcag ataaaccgcc
    10741 tcgtcatgca gacgggtcag aatgtcgcga tacagcgcct gacgttgcgt ttcgtcatgg
    10801 gtcgccagca cttcgccgat ctctttatca atcagcggct tgtcggctaa tccttgctgt
    10861 gcctggaagt cagcgtgcga cggtacgcgc attgaactca ggaaggcgtg tggatcgtat
    10921 ggcgcgcccc aggtgcggtg gaaaatcatg ccaaaacgac cgtcgcgctg acgagcatag
    10981 atactgctct cttcttcgcc aatcagcgag acatctgcgc caatctggcg catatcagcc
    11041 tgaatgattt ccgccatcga tttgcttaac gcatcggtgc cgatgaacga cagttcaatg
    11101 cgcagcggct gaccattttt ctcgcggatg tctttgcccg caggcagcgt ccaaccggct
    11161 ttttccagca actctttcgc tttctgcgga tcgtactggc gcggtttcag gccgagatta
    11221 gcgtaaggca cagaaggggc aaacagggtg tcggcgacct gctgggtgcc atataacgcg
    11281 ttatcaatca gcgatttttt gtttaccgcg taattaagag cttcacgtac tgccagctcg
    11341 ttggtggggg ctttggcggt attgagcgcc agcatcacgg tttcgatcgg ctgtgacagt
    11401 tgggtgtggt aagccggatt ctggctaaag cgggcgaagg tatcgagcgg taataaccct
    11461 tcgtttccgt acagcaggtc gatatcgcca gtttcaaacg ccaccgcgcg ggtagtcggg
    11521 tccgggatga cgttaaaggt gatcttttta atcgctggct tttcgcccca gtagttttcg
    11581 ttacggacga agacatcgta ctgattcagt ttcgattcct gcaaaatcca cggtccggtg
    11641 ccaatcggcg ctttaattcc gttcatggtt tcatggtttt taaactgcga gggagcgata
    11701 aaacggaaag gacggggcag ggccagttct tgcaggaaag gatagtaggc gcttttcagg
    11761 gtaatttgca gctctgtttt actgagtgct ttaacatcaa caatctggtt tgccagctcc
    11821 agccaggcgt gacgttgacg gttatcgagc actgcgcgga agttttctgc cgccgcctcg
    11881 gcatcgaacg gttcaccgtt ggagaatttc acgtcatcac gcagggtgaa ggtccaggtt
    11941 ttaccatctt ctgaatgagt ccagcttttt gccagccacg ggatcaccga accgtctgcc
    12001 tgatatttca ccaatggttc ataaaccatg ctctgggcga acatctggtt aggcgtgtaa
    12061 aggtgcgggt ttagtggccc gacattcacc ggccaggcgg tggtgatttc atctggtgca
    12121 gcggcatgga cgataaaaga cgcacaagcc agcagcgcaa atagagtgcg gcggagtgtg
    12181 gagagcatgg taaccccaat ggattaaaat agatggcgga aataagtatg acgattttaa
    12241 gtattcgtca tactgattac ctgttctgga tcaatagatg ggcagtcggg tgggcggatc
    12301 agttaactga atcgatccat tgcacactgt cggcggtgag cgtaaagggg gtgggggtgc
    12361 agatcgccag gcttaaattt tcaagctgac aatggctgac tgacagcgag gagtgatagg
    12421 tgctgtctac gctgacgatt tgccaggcgc tgccgccacg ctgtttaacg atggcttctt
    12481 tgcgcgtcca gatgcgccag aacatttcca gttgctgatc aggatgcacg gcgtccatct
    12541 cagcgtgttc cccgaggctg aatacggcgt tcgccagcca gcgccagttg gcgcgcgggc
    12601 gaatcacttc gatatcgcag ccgacttcgc cttcatcact caacagcaga gcgatatcgt
    12661 caccgctatg gcttaagttg aaccatagcg gcgtttccgg cgcgaatgca ggtttgcctt
    12721 gttcgccata gatgatctcc ggtagcgggg aaagcgtgtg cgaaagcaat gcacgccccg
    12781 ccagccagcg ttcgcgtcgt ggaccttgcg gtgcttgctc gcgtaaaccc ggtggcagtg
    12841 gagctgcgct taaggtcgaa actttcccca gaactatccg atacatatca gggccaacgt
    12901 ttaatggaaa atgaaagtgc gtatcgtatc acttgtcgcc tcatcccggt aaccgacttt
    12961 tcggtctgcc cggccccagt aaaatcgcca gtttgctgcc acctttcgtc gtttccatcc
    13021 agattttaca cacgctggtt aatggcaccg aaagtagcat ccctaccggg ccgagcagcc
    13081 atccccagat taacaatgaa agaaatacca ccatggtgga catccccagg cgatggccca
    13141 tcatccgtgg ctctaaaata ttgccgatga ccatatggac aactaaaaac aatgcgccga
    13201 ccagaataca ttcgtaaata ccattaaaca gcagcacctg aatcattggc ggtacggcgg
    13261 aaattaccgc gccgatattg ggcacgtagt taagcaaaaa cgccagtact gcccacatca
    13321 gcgcaaactg tacccccatc agctccagcc ccagccagac gatgacacct gtccataaac
    13381 tgagtagcgt cttcaatgca agatagtgcg aaacgccttt aagtgcgcgg tgtaatcccg
    13441 cgatgtgaat ctgtgggtta ttcagcgcaa aacgcatttt gtaagggacg tggcgcactt
    13501 caaacagcat aaaaactacg gtcatcacca gcaaaagcac gctcgccatt gccccggaaa
    13561 gcccggtcat tagcgctgtg gtgaaggtaa ccactttttc cgagtccatc cgctgcagca
    13621 ttcgctccgg cgacatatgc aaattaagaa aaggcaacat ctcctgcaat ttaaaaagtt
    13681 tgcgcgtcaa ctccttatta aacttcggca gcatagagat aaattcgtta aacgatgccg
    13741 ccagtacgcc gaccagcgca gttagtgcga tcagcatcac caccaccaca atcgtaatgg
    13801 caatggggcg ttgtactccc cgacgaataa accaggtgac gagcgggttg aggacgatgg
    13861 caaaaaacag cgccagtaac agctgcacaa tgatatctgc cgctgcgtga atgcccgcga
    13921 ggatcactac cagcgaggcc agcttgagca gaatgtgcat gcccgtttta tcgggttgag
    13981 gggtttccat tggggcttcc ttgtgacttt ttgtattaag tgtagcggga gtcacgccag
    14041 caatattctg attcttactg ctgattttca cgtcagcacg tggtaaaaat gaaacactgt
    14101 tgtaaaaatg tggtgatcct catgcccgaa cccgtagccg aacccgcgct aaacggattg
    14161 cgcctgaatt tgcgcattgt ctctatagtc atgtttaact tcgccagcta cctcaccatc
    14221 gggttgccgc tcgctgtatt accgggctat gtccatgatg tgatgggctt tagcgctttc
    14281 tgggcaggat tggttatcag cctgcaatat ttcgccacct tgctgagccg tcctcatgcc
    14341 ggacgttacg ccgatttgct gggccccaaa aagattgtcg tcttcggttt atgcggctgc
    14401 tttttgagcg gtctgggata tctgacggca ggattaaccg ccagtctgcc cgtcatcagc
    14461 ctgttattac tttgcctggg acgcgtgatc cttgggattg ggcaaagttt tgccggaacg
    14521 ggatcgaccc tgtggggcgt tggcgtggtt ggctcgctgc atatcgggcg ggtgatttcg
    14581 tggaacggca ttgtcactta cggggcgatg gcgatgggtg cgccgttagg cgtcgtgttt
    14641 tatcactggg gcggcttgca ggcgttagcg ttaatcatta tgggcgtggc gctggtggcc
    14701 attttgttgg cgatcccgcg tccgacggta aaagccagta aaggcaaacc gctgccgttt
    14761 cgcgcggtgc ttgggagcgt ctggctgtac ggtatggcgc tggcactggc ttccgccgga
    14821 tttggcgtca tcgccacctt tatcacgctg ttttatgacg ctaaaggttg ggacggtgcg
    14881 gctttcgcgc tgacgctgtt tagctgtgcg tttgtcggta cgcgtttgtt attccctaac
    14941 ggcattaacc gtatcggtgg cttaaacgta gcgatgattt gctttagcgt tgagataatc
    15001 ggcctgctac tggttggcgt ggcgactatg ccgtggatgg cgaaaatcgg cgtcttactg
    15061 gcgggggccg ggttttcgct ggtgttcccg gcattgggtg tagtggcggt aaaagcggtt
    15121 ccgcagcaaa atcagggggc ggcgctggca acttacaccg tatttatgga tttatcgctt
    15181 ggcgtgactg gaccactggc tgggctggtg atgagctggg cgggcgtacc ggtgatttat
    15241 ctggcggcgg cgggactggt cgcaatcgcg ttattactga cgtggcgatt aaaaaaacgg
    15301 cctccggaac acgtccctga ggccgcctca tcatcttaaa acttactgaa taaccagcgt
    15361 attaatgatg ttttctgcgg tggtctgcgc tttttgctga tcgtcagcgg gcagcgtaat
    15421 ttgcatggtc agcagttgat tacccacgtt acccagaata acggaagagt acgccgtctg
    15481 gcctttcgcg gagataatac tgtctaactg ctgcattttg tgacctttca gctcaatggc
    15541 tttattggtt accacttgca gctgcggatc gcggctacgt tgctgatctt ccagacgctt
    15601 cgccagcacc gccagatctt ctttcggatc atcgcccatg atgacgatga ctgctttctg
    15661 cccggtggcg tcggaccaga catgcatgtt attggcctgc gttcccagct taccgctctg
    15721 gtcggtcata tccgctggca gcgagaaact taacttgcca tcaagcaggt tgacgggatt
    15781 cccggtagcg ttactttccg cgaccgaacc ctgcgccgta gcgttagtgt ctttatcatc
    15841 acaggccgca agccccataa ccagcaggcc aattccgaca tatttaacca gattgcgcat
    15901 tgacttcttc ctttcgataa acggccataa cggctcattc atccatctta tcacaactct
    15961 gataacgaac ctttaactcg cctgcaaagc gttgatttcg gatttatctg ccagtctttt
    16021 caacagcata ttgagtaata cgccatacat tggcaggaag aaaacgatac tgattaacac
    16081 tttgaaacag taatcgacca gcgcgatttc catccagtgt tcagccataa aggcatccgg
    16141 gctacgccag aaggcaatga agaaaaaggc cagcgtgtca ctgacgttac cgaacagtgt
    16201 ggacgctgtc ggtgccagcc accagcggcg actttgacgc aggcggttaa aaacgtgcac
    16261 gtcgaggatt tgccccagcg cgtaggccat gaaactggcg gtggcgatac gggcgacaaa
    16321 caggttgaag tgggcgagtg cgccgaatcc ctgccatgaa cccatataga acagcgacga
    16381 gatgacatag gagattaata acgcagggat cattaccgcg aagataatgc gtcgggccag
    16441 cggtgcgcca aaaatacgca cggtcaggtc ggtcgcgaga aagataaacg gaaaactaaa
    16501 cgcgccccag gtggtatgaa aaccaaaaat ggagaccgga agctggacca gatagttact
    16561 ggaggtgatc accagcagat gaaataacga taaccagaac aacgccttat agcgttgagt
    16621 ttgcgagaaa acgttcatat tgtacctttt tgattaacca ttggggtgag ggaacccaat
    16681 acgtacgaca cgtctgttat cttaacataa taacgtgctt accctttttt cgagccgccg
    16741 catgatactg ctttacgttg acaatgcaat ggttaatttt cacgcaatcg ttaacctggt
    16801 ttgcttacgg actcacaggg cgtaaactag cgccgttttt ttaagtgatg agaagaaaat
    16861 gaccgatctc ttttccagcc ctgaccacac actcgacgcg cttggcctgc gctgcccgga
    16921 accggtgatg atggtgcgca aaaccgtgcg caatatgcag cctggcgaaa ctttactgat
    16981 tatcgccgac gatccggcga cgacccgcga tattcctggg ttttgtacct ttatggaaca
    17041 cgaactggtt gcgaaagaga cggatggact gccttatcgt tatttgattc gtaaaggcgg
    17101 ttgatgggga aagattggct tcgatgccgc cttttcccct caccctaacc ctctccccag
    17161 aggagcgagg ggaccgatcg cgctcaatgt tgcgatcggt ttgccttatc tcctgcgcaa
    17221 caatcttaac gcattcgctg ttaccagcac ggtcgcaccg gtatctgcca gcaccgccag
    17281 ccatagtccg gtcatcccta gcagcgtggt gacgaggaag atccctttca gccccagcgc
    17341 aatagtgatg ttctggcgga tattggcgtg agtggcgcgg gccagttcaa tcatttgcac
    17401 caggccgcgc aggtggttat gggttaatgc tgcgtcggcg gtttccagcg ccacgtctgt
    17461 gccactgccc attgcaatcc caatggcggc agctttcatc gctggcgcgt cgttaatacc
    17521 atcaccgacc atcgccaggg gcgcatgttg attcagcttg gtcaccgctt tgactttatc
    17581 ttccggcaat aggcccgctt taaactccag ccccagctcc ccggcaattg ccgccgctgc
    17641 gcgtggatta tcgccggtga ggatcactcc tttaaccccc agcgcgttca gttcactgat
    17701 ggcagttgct gcgtcggcgc gcagggtatc ctgcaatgca atgataccca gcacgtcatc
    17761 attacgtact accagcacaa ccgtttgccc ggcgctttcc agttcattaa tcaaaccagc
    17821 aaatgcatca gcgggatgtt tcccggcagc gcaaatcaat acgcgctcac cgttaacctg
    17881 cgcttcaatg ccagacccga ccagcgcccg ctgtgattcg gcggtgggaa tggcgagttc
    17941 agcaacctgt gcttcgcgta cgatggcctg cgccagtgga tgcgtcgcgc cttgctcgac
    18001 cgccgccgcc agtgtcagca gttcagattc actaataccc gttgccggat gaatcgcggt
    18061 aacgcgcggt ttaccgacgg tcagcgtacc ggttttatca aacgccacct gagtaacacg
    18121 acccagctgt tccagcgccg ctccgccttt aatcaacgcc ccacgacgcg ctgccgccgc
    18181 cagcccggag gtaatcgccg caggcgtgga gataactaac gcacacgggc agccaatcag
    18241 cagcagcgtc agccctttat aaatccactc ctgccagctg gcggcaaaca gcagcggtgg
    18301 caccagcgtc accagcaggg cgacggccat aatcgctggc gtataaatac ggctgaaacg
    18361 gtcgataaac cgctcaatgg gggcgcggcg ctcttcggct tcttcgatca gtttcagaat
    18421 ccggtcaatg gcgctggcac ccggttctga cagcacttcc agcgttacca gacggtctac
    18481 gctggtggct cctgcaggaa ctttatcgcc cgtcgcgcgc tccaccggaa tggattctcc
    18541 ggtcagggcg ctttcatcaa aactggcaaa cggtgagagc agtttaccgt cggcaggcaa
    18601 acgcccacct gcggcgactt caatcacatt gccaggccgc aggctgttaa tcgccacctc
    18661 ttcccgctca ccgttacgca ggcgcgtggc ggtttctggt ttcagcgcca ttaacgcgct
    18721 aacgccctga cgcgcgcggc tggcggccca gccttccagt cgttcaccaa tcaaaaacag
    18781 cagcaacacc atcgcagctt cagccgttgc gccaataaac agtgcaccaa tagcggctac
    18841 gctcattaag gtttcaatgg cgaagtagct gccggatttg atcagccgta atgcctgacg
    18901 agcaatcggg tacagcccaa ccagcgtggt cgcgataaac gccagttgcc caaacggatg
    18961 attaaattgc tccagacccc agctgattgc catcatcacg attagcgtaa tcagcggcag
    19021 attctctttc aggcgcgatg cttgtggctc gtcggcggcc tgctcatcgc gcagggaata
    19081 gcccgctttt tgcaccgcag attcaacctg ggcacggatg tcattgtcgg catcgaccac
    19141 cagtttttcg gtggcgaaca gtacctgcac ctgattcaca cctgcaagct ggcgcacggc
    19201 attttctacc ttgcgcgcac aggcggcgca gtccatgccg ctgactttcc agctatagcg
    19261 ggtgccggag acgttttcag agagcgttgg cgagctggaa catgcgccgt cgcagcaaca
    19321 gtcgttggcg ttctgtaccg tggttagcgg tttgaacgca gcaaattgag gggctttctt
    19381 gccgtgattg tcaggagtcg acatggcatc ctccggttaa gttttttctc attaaccgaa
    19441 ggatacactc tggagtcgac tccagagtca agttttatca gagatacagc gagcggacga
    19501 tgaggaaatg accggcaaag tagcaggccg cagcaatcgc gttatctgcc cggaagcggc
    19561 ggcgatagtg gctacccagc cagacaaagt tactgataaa cagcaatgaa gcgccaacaa
    19621 acgcagagag tgccggagcg gtcggacgga agaaccacag ttcacctgcc agccacacca
    19681 tcaccagcgt catgccgata aacgtgcaga taggccaacg atactcttcc agccgcgtcc
    19741 agataatcgc cagtaacagc gcgcccagaa ccagcaacac cagtggcaga ggccagaaga
    19801 aagagagcgt catctgactg gcgaaataga tggtgtacag caggtgcgag aggaaaaacg
    19861 cgccgatggc gtacatcaga cgttgacgtg gcaacagggt tagcgcatcg cccagcagtg
    19921 aggcgcacag ccctgccagc accagatagc taatggcgtc gaacatcggc gcttgccagg
    19981 ccagtaacag caggagaaga agggttaacg gtttaaacac ccagcgttgc caggttggcc
    20041 cacgatacga tgcatccaca gatagccatg cggaaagaca gacagcgata aacgaccaaa
    20101 gcatcttagt tccttgaatt cgttattttt tcgtaagcgg aatgcctacg gtcttgcttc
    20161 cagtttagtg gcgagggtgg acggatgaca acaccataac tggcagggta tgcttatttc
    20221 cgcattttcc gatgagggat aaagatgagc aaaccacctc ttttctttat tgttatcatt
    20281 ggcttaattg tcgtcgccgc atcgtttcgt tttatgcaac agcgacggga aaaagctgat
    20341 aatgatatgg ctccgctcca gcaaaagctg gtggtggtga gcaacaagcg ggaaaaaccg
    20401 attaacgatc gccgttcgcg ccagcaggaa gtgactccgg caggcaccag tatgcgctat
    20461 gaggcaagct tcaaaccgca aagcggagga atggagcaga cgtttcgcct cgacgcccag
    20521 cagtaccacg ccctgacagt gggcgataaa ggtacgctga gctataaagg aacgcgcttt
    20581 gtcagctttg taggcgaaca ataaggttat tttttaactt taaatttctt ctgccagacc
    20641 agcagttcaa acacgccgaa aaggaaaatc cgcacctttt cggcggtggt catctgcggg
    20701 ccatctttcg gtaaagtgga tttcaatagc gccagttgca taccatgcat cagcacggta
    20761 aaaatcagcg ccacgttaac gaaaatattc agcgggcgcg ggaagggatg caccagattg
    20821 aggattaaaa atccccaaac gcagagcatt aacaaacgac caatattaat cagcatcact
    20881 ttctccttgt gcttcgcgtt gatacagccg ataagccacc tgacccgcca ctttttcccg
    20941 atgtaatgac cagtttgccg gaacagtggg cagaccgttt tcgacttcgc tttcgacata
    21001 aatcagggct tcgtcagcca gccagccgtt atcttccagt aaatttatcg tctcttctaa
    21061 caagccacgg cggaacggtg gatcgacaaa cacgatatta tgcggtgtgc ctttttgcgc
    21121 caggaatgac attgcgttgc tgttcaccac gcgtgcattg cctgctttta gtgtcgccag
    21181 attcttaatt aactgctgag aaactgcgcg atccatctca atcaacgtgg cccccgcagc
    21241 gtagcgcgat aacgcttcca gccccagcgc gccgctcccg gcgaagcaat ccagacattg
    21301 ggcgtcaaca atgaccggag ccagccagtt aaacaacgtt tcgcgtacgc ggtcggtggt
    21361 ggggcgcaga cctgggctat caggaaccgg gagtttacgg cctcgccact gcccgccaat
    21421 aatgcggatt tggccgctgc cggaatgatt cggttttttc atgataaatt gctcaatccg
    21481 ccagataaca catacttgca ggcggtgatg tgaattaagt taagtgatag actatttcat
    21541 cattttttta gctgctatgt acatagcgtt aacgctgtgc catgaagcaa cagcgaggag
    21601 tgtagtcgca aatggcgaaa gaaaaaaaac gtggcttttt ttcctggctg ggctttggtc
    21661 aaaaagagca gaccccggaa aaagagacag aagttcagaa tgaacaaccg gttgtagaag
    21721 aaatcgttca ggcgcaagag cctgtgaagg cttctgaaca agccgtcgaa gagcagccgc
    21781 aggcgcatac tgaagccgag gcggaaactt ttgctgccga cgttgtggaa gtcactgaac
    21841 aggttgctga aagtgaaaaa gcgcagcctg aagcggaagt cgttgcacag ccggaaccgg
    21901 tcgtagaaga aacgccggag ccagtggcta tcgagcgtga agagctgccg ttgccggaag
    21961 acgtcaacgc cgaagaggtt tcgccagaag agtggcaggc tgaagcggaa accgtagaga
    22021 ttgtcgaagc ggcggaagaa gaagcggcta aagaagaaat caccgacgaa gagctggaag
    22081 cacaggcgct ggctgccgaa gcggcagaag aggcggtgat ggtggttcct ccggtagaag
    22141 agcagccggt ggaagaaatc gctcaggagc aggaaaaacc gaccaaagaa ggtttcttcg
    22201 cgcgcctgaa acgcagcctg ttaaaaacca aagaaaatct cggttccgga tttatcagcc
    22261 tgttccgcgg taaaaaaatc gacgatgatc tgtttgagga gctggaagaa cagctgttga
    22321 tcgccgatgt gggcgtggaa accacacgta aaattatcac caatctgacg gaaggcgcat
    22381 cccgcaagca gcttcgtgat gccgaggcgc tctatggcct gctgaaagaa gagatgggcg
    22441 agattctggc gaaagtcgat gagccgctga atgttgaagg taaaacgccg ttcgtgatcc
    22501 tgatggtggg cgtcaacggt gtgggtaaaa ccacgacgat tggtaagctg gcgcgtcagt
    22561 ttgagcagca gggtaaatcg gtgatgctgg cggcgggcga tactttccgt gcagcagcgg
    22621 ttgaacagct tcaggtctgg ggtcagcgca acaatattcc ggtgattgcc cagcatactg
    22681 gtgcggattc cgcctctgtt atcttcgacg ccattcaggc agctaaagcg cgtaatatcg
    22741 acgtcctgat tgccgatacc gccggacgtc tgcagaacaa atcgcatctg atggaagagt
    22801 tgaagaaaat cgtccgcgtg atgaagaaac tcgacgttga agcgccgcat gaagttatgc
    22861 tgactattga tgccagcacc gggcagaacg cggtaagcca ggccaaactg ttccacgaag
    22921 ctgttggctt aaccggcatc acgctaacga aactggacgg cacggcgaaa ggcggggtaa
    22981 ttttctcggt ggctgaccag tttggtatcc ctatccgcta cattggtgtc ggcgaacgta
    23041 ttgaggattt gcgtccgttt aaggcggacg actttataga ggcacttttt gcccgagagg
    23101 attaacaatg attcgctttg aacatgtcag caaggcttat ctcggtggga gacaggcgct
    23161 gcagggcgtt acgttccata tgcagccggg tgagatggcg tttctgaccg gtcattccgg
    23221 cgcagggaaa agtaccctcc tgaagctgat ctgtgggatt gagcggccca gcgccgggaa
    23281 aatctggttt agcggccatg acatcacgcg tctgaaaaac cgtgaagttc cgtttctgcg
    23341 ccgccagatt ggcatgattt tccaggatca ccatctactg atggaccgta ctgtctacga
    23401 taacgtggcg atcccgctga ttatcgctgg tgccagcggt gacgatattc gtcgccgggt
    23461 gtcggcggca ctggataaag tcgggctact ggacaaagcg aagaacttcc ctattcagct
    23521 ttccggcggt gaacaacagc gtgttggcat tgcccgcgcg gtggtgaata agcccgcggt
    23581 actgctggcg gacgaaccga ccggtaacct ggacgacgcg ttgtcggaag ggattctacg
    23641 tctgtttgaa gagtttaacc gcgttggggt aaccgtattg atggcaacgc acgacatcaa
    23701 cctgatttca cggcgttcct atcgcatgct caccctgagc gatggtcact tgcatggagg
    23761 cgtgggccat gaataagcgc gatgcaatca atcatattcg gcagtttggt gggcgtctgg
    23821 atcgcttccg taaatcggtc ggcggctcag gcgacggcgg tcgtaacgca ccgaaacgcg
    23881 cgaaatcctc gccaaaaccg gtaaatcgca aaaccaacgt tttcaacgaa caggtgcgct
    23941 atgccttcca cggcgcattg caggatctga aaagcaaacc gttcgccacg tttttaacgg
    24001 tgatggttat cgccatctcc ctgacgctgc ccagcgtctg ttatatggtg tacaaaaacg
    24061 ttaatcaggc ggcgacgcag tattatccgt caccgcaaat cactgtttac ttgcaaaaaa
    24121 cgctggacga tgacgccgct gcgggcgtgg tggcacagtt gcaggccgag caaggcgtgg
    24181 agaaagtgaa ctatctttct cgtgaagacg cactgggcga gttccgtaac tggtctggtt
    24241 ttggtggtgc gctggatatg ctggaagaaa acccgcttcc ggcagtggcg gtggtgatcc
    24301 cgaaactcga ttttcagggg acggaatcac tcaatacgct gcgtgatcgt atcacgcaga
    24361 ttaacggcat tgacgaagtg cggatggatg acagctggtt tgcccgtctg gcggcgttga
    24421 ccgggctggt cgggcgcgtt tcggcgatga tcggcgtgtt gatggtggcg gcagtgttcc
    24481 tcgtcatcgg caacagcgta cgtctgagca tctttgctcg ccgtgactcc atcaacgtgc
    24541 agaaactgat tggtgcgacg gatggtttca tccttcgccc gttcctgtat ggtggcgcac
    24601 tgctgggatt ttctggcgca ttgttgtcat taattttgtc agaaattctg gtgctgcgat
    24661 tgtcatcggc ggttgcggaa gtggcacagg ttttcggaac gaagtttgat atcaatggtt
    24721 tatcgttcga tgaatgcctg ctattgctgc tggtatgctc gatgattggc tgggtggcag
    24781 cgtggcttgc cacggtacaa catttacgcc actttacgcc tgaataataa aagcgtgtta
    24841 tactctttcc ctgcaatggg ttccgtagca gggaaagaga tcccgttgtc tcttcccggt
    24901 atttcatctc tatgtcacat tttgtgcgta atttattcac aagcttgcat tgaacttgtg
    24961 gataaaatca cggtctgata aaacagtgaa tgataacctc gttgctctta agctctggca
    25021 cagttgttgc taccactgaa gcgccagaag atatcgattg agaggatttg aatgactgac
    25081 aaaatgcaaa gtttagcttt agccccagtt ggcaacctgg attcctacat ccgggcagct
    25141 aacgcgtggc cgatgttgtc ggctgacgag gagcgggcgc tggctgaaaa gctgcattac
    25201 catggcgatc tggaagcagc taaaacgctg atcctgtctc acctgcggtt tgttgttcat
    25261 attgctcgta attatgcggg ctatggcctg ccacaggcgg atttgattca ggaaggtaac
    25321 atcggcctga tgaaagcagt gcgccgtttc aacccggaag tgggtgtgcg cctggtctcc
    25381 ttcgccgttc actggatcaa agcagagatc cacgaatacg ttctgcgtaa ctggcgtatc
    25441 gtcaaagttg cgaccaccaa agcgcagcgc aaactgttct tcaacctgcg taaaaccaag
    25501 cagcgtctgg gctggtttaa ccaggatgaa gtcgaaatgg tggcccgtga actgggcgta
    25561 accagcaaag acgttcgtga gatggaatca cgtatggcgg cacaggacat gacctttgac
    25621 ctgtcttccg acgacgattc cgacagccag ccgatggcac cggtgctcta tctgcaggat
    25681 aaatcatcta actttgccga cggcatcgaa gatgataact gggaagagca ggcggcaaac
    25741 cgtctgaccg acgcgatgca gggtctggac gaacgcagcc aggacatcat ccgtgcgcgc
    25801 tggctggacg aagacaacaa gtccacgttg caggaactgg ctgaccgtta cggtgtttcc
    25861 gccgagcgtg tgcgccagct ggaaaagaac gcgatgaaaa aacttcgcgc tgccattgaa
    25921 gcgtaatttc tactactaag cagagaaccc tggatgagaa tccggggttt ttgttttttg
    25981 ggcctctgca ataatcagtt tccccctccg gcaaaacgcc aatccccacg cagattgtta
    26041 ataaactgtc aaaatagcta ttccaatatc ataaaaatcg ggtatgtttt agcagagtat
    26101 gctgctaaag cacgggtagt catgcataaa acgaaataaa gtgctgaaca acaacatcac
    26161 aacacacgta ataaccagaa gaatggggaa cctcaggatg aacatgaagg gtaaagcgtt
    26221 actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt
    26281 cgcggtcgtg ggcgcaatgt ccggtccggt ggcccagtac ggtgaccagg agtttactgg
    26341 cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca
    26401 aatcgtgaaa tatgacgatg cctgtgaccc aaaacaggcg gttgcggtgg cgaacaaagt
    26461 cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagccagc
    26521 gtcagacatc tatgaagatg aaggcatttt aatgatcacc ccagcggcaa ccgcaccaga
    26581 gctgaccgct cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg
    26641 gccgacggcg gcgaaatata ttcttgagaa ggtgaaaccg cagcgtattg ctatcgtcca
    26701 cgacaaacag cagtacggcg aaggtctggc gcgtgcggtc caggacggcc tgaagaaagg
    26761 caatgctaac gtggtgttct ttgatggcat caccgccggg gaaaaagatt tctctacgct
    26821 ggtggcgcgt ctgaaaaaag agaatatcga cttcgtttac tacggcggtt atcacccgga
    26881 aatggggcag atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc
    26941 ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct
    27001 ggtgaccaag ccgaagaact acgatcaggt tccggcgaac aaacccattg ttgacgcgat
    27061 caaagcgaaa aaacaggacc caagcggcgc gttcgtctgg accacctacg ccgcgctgca
    27121 atctttgcag gcgggcctga atcagtctga cgatccggct gaaatcgcca aatacctgaa
    27181 agcgaactcg gttgataccg tgatggggcc gctgacgtgg gatgagaaag gcgatctgaa
    27241 aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcaaccg atgcgaagta
    27301 acagatagat aaatgaaaat gcccgatgcg ctgaactgcc atcgggtttt tttgtgtttg
    27361 tcgaaatggt tgtagaaaga ttaaacaatc caggatatgc ttaatgtacg gtgacatacc
    27421 gtacatatta tacggagcag gagagttcac atgtctgctg ttaaaaagca gcgtatcgat
    27481 ctgcgtttaa ctgacgatga caaaagtatg attgaggaag cggcagcgat atctaatcag
    27541 tccgtcagcc aatttatgtt gaacagcgct tcgcagcggg ctgcggaagt gattgaacag
    27601 catcggcggg tgatcctcaa tgaggaatcc tggacgcggg tgatggatgc gctgagtaat
    27661 ccaccgtcac ctggtgaaaa gctaaaacgt gcggcaaaac gtcttcaggg aatgtaataa
    27721 tttatggatg atctgacgat agagattctg accgatgatg cagattatga tctacagcga
    27781 ttcgactgcg gcgaggaagc gttaaatctc tttctgacga cacatctcgt tcgtcaacat
    27841 cgcaacaaaa ttctgcgagc gtatatcctt tgtcgcaaca ctccagaacg tcaggtgctg
    27901 ggatattaca cattatgcgg cagttgtttt gaacgagccg cattgccctc gaaatcgaaa
    27961 cagaaaaaaa ttccctacaa aaatattccc agcgttactc ttgggcgtct ggcaattgat
    28021 cgttcattac aggggcaggg atggggagca acactggttg ctcatgccat gaaagtcgtc
    28081 tggtcagcct ctttagcggt aggtattcac ggtctttttg tcgaggcgct gaataaaaaa
    28141 gcccatacgt tttatcaatc gctgggcttt atcccgttgg tcggagaaaa cgaaaatgcg
    28201 ttatttttcc caaccaaatc catcgaactg cttttcacac agagcgatta acgcttctcc
    28261 cagccgccct gttgcgccgt aaatcccagt gcctgcataa acgccgtcat cacaccgcga
    28321 tcttccacgc ctgcatccgc catccaccag catgaaacgc caggattgtt acgcaaaacc
    28381 tcttccagca gatattgccc cacaccgcga cggcgggtga cttcccgcac gcacagggaa
    28441 tccagtgctc cctcggtgcc gcttaaggtc acccgcacgg ccgctagcag gcgctcgtta
    28501 aaacgcgcgg cgtagatacg gtggttatcg tcaacctgta aagaggaagg ggaatactcc
    28561 ggccagatct tttgcaggtc aatccggtcc tggtcgctaa agttttctaa tcgaatgatg
    28621 gtcagcttca tggggaaccc gtgtaaatca caaaagtgga accagtgtag cgaaataatt
    28681 taatcggagg ctttctc
//
LOCUS       DAPHJE010000055        28505 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPHJE010000055 DAPHJE010000000
VERSION     DAPHJE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.055"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..849
                     /locus_tag="RH222_002409"
     CDS             <1..849
                     /locus_tag="RH222_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV3797411.1"
                     /translation="NMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNA
                     NSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEH
                     SYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISL
                     QNCVRVTNIETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKP
                     NDSNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATST
                     FQVTYP"
     gene            962..1756
                     /gene="panB"
                     /locus_tag="RH222_002410"
     CDS             962..1756
                     /gene="panB"
                     /locus_tag="RH222_002410"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV3797412.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1768..2619
                     /gene="panC"
                     /locus_tag="RH222_002411"
     CDS             1768..2619
                     /gene="panC"
                     /locus_tag="RH222_002411"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV3797413.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2693..3595)
                     /gene="rpnC"
                     /locus_tag="RH222_002412"
     CDS             complement(2693..3595)
                     /gene="rpnC"
                     /locus_tag="RH222_002412"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV3797414.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            3869..4249
                     /gene="panD"
                     /locus_tag="RH222_002413"
     CDS             3869..4249
                     /gene="panD"
                     /locus_tag="RH222_002413"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV3797415.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4253..5482)
                     /locus_tag="RH222_002414"
     CDS             complement(4253..5482)
                     /locus_tag="RH222_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV3797416.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLMSNQPQG"
     gene            complement(5546..5986)
                     /locus_tag="RH222_002415"
     CDS             complement(5546..5986)
                     /locus_tag="RH222_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3797417.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(6091..6861)
                     /locus_tag="RH222_002416"
     CDS             complement(6091..6861)
                     /locus_tag="RH222_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3797418.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6858..7784)
                     /locus_tag="RH222_002417"
     CDS             complement(6858..7784)
                     /locus_tag="RH222_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3797419.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7893..8555
                     /gene="can"
                     /locus_tag="RH222_002418"
     CDS             7893..8555
                     /gene="can"
                     /locus_tag="RH222_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV3797420.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8596..9132)
                     /gene="hpt"
                     /locus_tag="RH222_002419"
     CDS             complement(8596..9132)
                     /gene="hpt"
                     /locus_tag="RH222_002419"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV3797421.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            9338..11728
                     /gene="gcd"
                     /locus_tag="RH222_002420"
     CDS             9338..11728
                     /gene="gcd"
                     /locus_tag="RH222_002420"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV3797422.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWLSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKNPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11930..13480)
                     /gene="cueO"
                     /locus_tag="RH222_002421"
     CDS             complement(11930..13480)
                     /gene="cueO"
                     /locus_tag="RH222_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV3797423.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13646..13993
                     /locus_tag="RH222_002422"
     CDS             13646..13993
                     /locus_tag="RH222_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV3797424.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            14099..14965
                     /gene="speE"
                     /locus_tag="RH222_002423"
     CDS             14099..14965
                     /gene="speE"
                     /locus_tag="RH222_002423"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV3797425.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14981..15775
                     /gene="speD"
                     /locus_tag="RH222_002424"
     CDS             14981..15775
                     /gene="speD"
                     /locus_tag="RH222_002424"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV3797426.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15813..16175)
                     /locus_tag="RH222_002425"
     CDS             complement(15813..16175)
                     /locus_tag="RH222_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV3797427.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16350..18947)
                     /gene="acnB"
                     /locus_tag="RH222_002426"
     CDS             complement(16350..18947)
                     /gene="acnB"
                     /locus_tag="RH222_002426"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV3797428.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            19302..21116
                     /locus_tag="RH222_002427"
     CDS             19302..21116
                     /locus_tag="RH222_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV3797429.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHRDGNGWQHNGDNINIDVNNFNR
                     ITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQ
                     AAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFSRRQPLTQQQKDAA
                     RQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQAVREKMQTNPQIQ
                     QRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQ
                     ESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(21187..22611)
                     /gene="lpdA"
                     /locus_tag="RH222_002428"
     CDS             complement(21187..22611)
                     /gene="lpdA"
                     /locus_tag="RH222_002428"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV3797430.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22819..24711)
                     /gene="aceF"
                     /locus_tag="RH222_002429"
     CDS             complement(22819..24711)
                     /gene="aceF"
                     /locus_tag="RH222_002429"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV3797431.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24726..27389)
                     /gene="aceE"
                     /locus_tag="RH222_002430"
     CDS             complement(24726..27389)
                     /gene="aceE"
                     /locus_tag="RH222_002430"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV3797432.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27550..28314)
                     /gene="pdhR"
                     /locus_tag="RH222_002431"
     CDS             complement(27550..28314)
                     /gene="pdhR"
                     /locus_tag="RH222_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV3797433.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6880 a         7728 c         7000 g         6897 t
ORIGIN      
        1 aacatgtggt cagcttacgg taccgtaact aacgttagtt cacctgggat atacattggt
       61 gactctgcag aacaatattt ttcgtggtat aatccaagcg aagacgtgtt atattggagt
      121 tgcaataatg cgaatagcac ccgtaaatac tgggctgtag gtggtattta tcagaccctt
      181 acaattgaat tctatacaga tacaaacttt gatccaacag taactcaaca gatcaaatta
      241 tccagctcat caaattatct gtattcattt aaagcctatg gcgcaggcca aggtataaat
      301 gagcatagtt attttatcaa aatcgatttc gatctgctca atgtcaagtt gactaatccc
      361 acttgtttta ccgctatgct tagtggaact tctgtgactg gttctacggt aaaaatgggt
      421 gaatatagtg cagaacaaat cagaaacggt gccacaccgg ttccttttga tatttcactt
      481 caaaactgcg ttcgtgtgac taatattgag acaaaattag tttcaacaaa ggttggtact
      541 gaaaacgggc aactccttgg taatactctc acaggtaatg acgcagcaaa aggagtcggt
      601 gtactcatag aaggtttagc aactagtaaa aatcctctaa tgacattgaa acctaatgat
      661 tcaaattctg tttataaaga ttacgacccg agaggcaaag acgatacaac aggaggagtt
      721 tacccggatc aagatacagg tataacatac cctctccatt tccaggccac gctacaacag
      781 gatggaacta taccaataga agctggtgag tttaaagcca ccagtacttt ccaggtaacc
      841 tacccttaat aagtccaccg caccgccatc atctggcggt gcagtaattg ataaatcatt
      901 gcccccacca atgtgactga taaaatgcca ggcaacacga catcatttat caggatacgt
      961 tatgaaacca accaccattg cttcacttca gaaatgtaag caggataaaa agcgtttcgc
     1021 gaccatcacc gcttacgact acagcttcgc gaaactattt gctgatgaag gacttaacgt
     1081 catgctggta ggcgactcgc tgggcatgac ggttcagggt cacgactcca ccctgccggt
     1141 gaccgttgct gatatcgcct accacactgc cgccgtacgt cgcggcgcgc caaactgcct
     1201 gctgctggct gatctgccgt ttatggcgta tgccacgccg gaacaagcct ttgaaaacgc
     1261 cgcaacggtt atgcgtgccg gtgccaatat ggtcaaaatt gagggcggtg agtggctggt
     1321 cgaaaccgta aaaatgctga ccgaacgtgc cgttcctgta tgtggtcact taggtttaac
     1381 cccacagtca gtgaatattt tcggtggcta caaagttcag gggcgcggcg atgaagcggg
     1441 cgatcaactg ctcagcgatg cattagcctt agaagccgct ggggcacagc tgctggtgct
     1501 ggaatgcgtg ccggttgaac tggcaaaacg tattaccgat gcactggcga tcccggttat
     1561 tggcattggc gcaggcaacg tcactgacgg gcagatcctc gtgatgcacg acgccttcgg
     1621 cattaccggc ggtcacattc ctaaattcgc taaaaatttc ctcgccgaaa cgggcgacat
     1681 ccgcgcggct gtgcggcagt atatggctga agtggagtcc ggcgtttatc cgggcgaaga
     1741 acacagtttc cattaaggag tcacgttgtg ttaattatcg aaaccctgcc gctgctgcgt
     1801 cagcaaattc gccgcctgcg tatggaaggc aagcgcgtgg cgctggtgcc tactatgggt
     1861 aatctgcacg atggccatat gaagctggtc gacgaagcca aagcccgcgc cgatgtggtc
     1921 gtcgtcagta ttttcgttaa cccaatgcag tttgaccgcc cggaagatct ggctcgttat
     1981 ccacggacct tgcaagagga ctgcgaaaag ctaaacaaac gtaaagtgga tttagttttc
     2041 gccccttcgg taaaagagat ctacccgaac ggtactgaaa cccacactta cgttgacgtt
     2101 cctggccttt cgaccatgct ggaaggtgcc agccgtccgg gacattttcg cggcgtttcg
     2161 actattgtca gcaagctgtt caacctggtc cagccggaca tcgcctgctt cggtgaaaag
     2221 gactttcagc aactggcgct aatccgcaaa atggttgccg atatgggctt cgatattgaa
     2281 attgtcggcg tcccgattat gcgcgccaaa gatggccttg cgctcagttc ccgtaatggt
     2341 tatctgacgg cggaacaacg caaaattgcg cccggtctgt acaaagtttt aagttcaatt
     2401 gccgacaaat tgcaggctgg ggaacgggat ctcgatgaaa ttattaccat tgcggggcaa
     2461 gaactgaatg aaaaaggctt ccgcgccgat gatattcaga ttcgcgatgc cgacacattg
     2521 ctggaagttt ctgaaaccag caaacgggca gtaattctgg tagccgcctg gcttggcgat
     2581 gctcgcctga tcgacaacaa aatggtcgag ctggcgtaat acttaactgg cgctacggct
     2641 gatggcgcca gttattaatt taccccacgt catctgtcat aacacaggta acttaaataa
     2701 ggttaattac cttatcaatc tcagcaagag ataaatttgt catctctgca acgtcttccc
     2761 gagacattcc tttacttaga agacgcaggg cgaattcttg cctttcctcc tgtcttccct
     2821 gctgaatccc cttctcaatc cctttctctt caaaccactg cgccagcgtc atcatagact
     2881 cccctcccgt ttccctgtct ctcagcacac cataaaacaa atccgcttgt tcagtatgac
     2941 cgcgttgcag catatagttt tgcatggcaa ctaactgact tccgctagtg tacccttcgt
     3001 caatcagcgt gaccagttgc tccagcaata acattaagtc gcgctggcga atatgttttt
     3061 gcagtagttc gagaatcgca atccgccgat gttgcatgat ttcgtcatcc ggtgtgatgg
     3121 tgatatccac cagcgggaaa ggactgttat agacgcgtcg tgccagctcc ggcgagtaaa
     3181 atatatcaaa ccagcacatt gagagcggat aaggtgtggc ctcgccctga taaaacagta
     3241 tcggcaccac cagcggcagc ttatcgtgat ccgcctccag atgccggtgc atggcagcaa
     3301 tagaataacg catcatgcga aaggccattt tcttatccgg cttgctttgg tgttcaatca
     3361 caacatgcag ataaccggga ttgccctgca tttgcacgga atagagcacg tccgtgctgt
     3421 gtcctttcag gctctcttca atgaaactcc ccgactctaa atgaagcgtg ttgaggtcac
     3481 aaagttcgcg taattccact ggcaaatgta tctccagaaa gtcgcgagcc gtctccgcat
     3541 gcattaaaaa ttgtttaaat accgcgtcat gcggtgtggt actcggtgca tccatcttcc
     3601 ctgtcttccg tgttgtcttg cgtgaaggca gtatccagtc aaggcgcgcc gtcgtcagcc
     3661 gcctctttag tgtttaccga gcagcgttca gagaaatttc aattcgttac aggcgataca
     3721 tggcacgctt cggcgcggta cgcaaactgc tgacgcaaat gccatcagat gagtagacac
     3781 tcatcaaaaa tcgggcaata ctgcgtgaga atttcctcaa agcaggcacc gcctgcttcg
     3841 ttaacgacag ggtagaaagg tagaagttat gattcgcacg atgctgcagg gcaaactcca
     3901 ccgcgtgaaa gtgactcatg cggacctgca ctatgaaggt tcttgcgcca ttgaccagga
     3961 ttttcttgac gcagccggta ttctcgaaaa cgaagccatc gatatctgga atgtcaccaa
     4021 cggcaagcgt ttctccactt atgccatcgc ggcagaacgc ggttcgagaa ttatttctgt
     4081 taacggtgcg gcggcccact gcgccagcgt cggcgatatt gtcatcatcg ccagcttcgt
     4141 taccatgcca gatgaagaag ctcgcacctg gcgacccaat gtcgcctatt ttgaaggcga
     4201 caatgaaatg aaacgtaccg cgaaagcgat tccggtacag gttgcttgat tgttatccct
     4261 gcggctggtt actcatcagc cgcgacatcg tctccagcga atccgttctt aagatataaa
     4321 gtcgttttag taacaacgga ttatcccccg gttttacttt gcctttcatg gttgtcaccg
     4381 ccaggtgaaa tccggcatcg tttgctgcct tcacggcttt gtcattaaat ccgccaaacg
     4441 gatacgaaag ataccagaca tgcggattaa attgcgccag agcgcggcgt gaacgtgcaa
     4501 aatcaaacag aatattgtgc tcactacggc tcagtaatat gggtcggcga tagccatcca
     4561 cccgatgcaa aaaatgggta tgtgactgaa aatcaaaaac atcgcgaatt tcgttaagct
     4621 cagaaacgct cataaattgc agcgattttg ggttccactt ctgcgggtga cgtttgatgc
     4681 gcgaggtaac aacaaacgcc gtcgccttca tgccatattg tttcaataca ggatacgcat
     4741 agcggctcac cgacttgagg ccatcatcaa aggtaatcac caccgctcgc gcagggagat
     4801 tgatcttatt cttcacgtaa ccttccagct gcgccatgct cagtgtcgcg tatcccctgt
     4861 cgcgcagcca ggccatctgg ttattgaaag cgcgtactga ggtggtcgtc gaagtatggc
     4921 gaaaacgggt gttttcttca tcgcgcagaa tatggtgata ggttagcacc ggcaggccat
     4981 tatcgggttg ggcatccagc gcgctgatat aggcgagtcg atcgccaata cggatctgat
     5041 accaggtttg atttaacctg tctttcagtt tatgcaaaat cgggtagcgc aaattgtccg
     5101 ccagtacccc aaatggcgca ctgcccgcac tcggcgcgtt atagaccggc gtatctttcc
     5161 aggtaattaa gttctgatta ctcagcggct tgttgaggtc acccaaaccg tcttcaactt
     5221 tttgtcgccc ctgaactggc tcgagatgcc ctttatcgat aaacccttta ccaaagccaa
     5281 aattaaatgc gtaataactt gcggcagtgg gctccacggc aatgatttgc ccggcccgaa
     5341 tattccccac ggtcaccatc ttgtcaccaa tttgcgccca gaccgcagca ttttcgatgg
     5401 tttgcatata acgggcaggt aacgcggcac taacgcttgc agtaaacagc atcagcagga
     5461 gaataacagc ttgtttgtac atgaaaaaat aaccgagggt gaaacaacgc ttttattgta
     5521 gcaaaagcgc catcagaatc gcgggttaat actcatacaa attatgtttg aggacgaaag
     5581 gcggattttt ttgttgttgg tgccaaagac tactcacctc cggcgcaccc agttcgacaa
     5641 tacgctcgcg aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta
     5701 acggtaacgt gacaccagaa atcacttcgc aacgggagtg tttgtggctt aataatgaag
     5761 ccacgcggta cggtggcgct ccggctatat cggttaagaa gatgacaccc tcaccagagt
     5821 ccgcttcatg cagagcatcg cacatcatgc ggctgagcat attagagctt aacccacgcc
     5881 agaaattcac ggcccggcac tgaagaagcg ccccatgttt tttctccagc gcatccagta
     5941 tctcttgcgc ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta
     6001 gccgaaaatc agtctaccct gaccattgtt gataaagggt gatagccgtc aaaactcctc
     6061 tcccccaaat ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat
     6121 cagcgaccaa cagatcaaat aaaacgccac aataaagacc accagtacgc caaaggtagt
     6181 gaccagcgga acatcattga taccgaggaa gccgtagcgg aaaccactga tcatataaac
     6241 gattgggttc aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gtgagtaaaa
     6301 gaccccaccc aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc
     6361 gaacgttttg gcaaacacac cgttcagcaa acccgcaagg gagaacaaca ccgccgtgag
     6421 caccagggtt aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga
     6481 aattgccgtc accagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc
     6541 ggcaataatg acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa
     6601 cttggcacca aaaaatgatg acgcaacgtt ggcgtaggca ttggtgatca ccgacatcat
     6661 gatcagcccc ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga
     6721 accaatcagg ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag
     6781 cgtctgcacc cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc
     6841 cacccagtaa agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac
     6901 agctcttcca gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt
     6961 aactgcgtaa atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg
     7021 gtatcgacca gtcgatactg atagccatcg agcttcggta acggactttt cggtgcgaga
     7081 tcgagaataa aggtttccga tttcagcttc gccagcagcg ccttcatcga ggtattttcc
     7141 accagctcac cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg
     7201 tagtgtgtgg tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac
     7261 attgagcggc gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta
     7321 ggttcatgca ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata
     7381 cgcgcacgtt cgttgcgttt tccccataga tcgagttgtt taagatattt ttcgctgcgg
     7441 atgtacgcct ctttgcgctc cacaccgtag taccctgcct gattcaccac aatttgctgc
     7501 acggtttcaa acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg
     7561 ttcacgacat ctttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt
     7621 accagagagc tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc
     7681 gcataaaaat cacccgcttc gacctgcaaa tctattccac gaagcgcctg aacgccgcct
     7741 ggataggttt ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct
     7801 tacgttctta cactttatct gtggtttaaa tcgtcccgga gttgccctat attagccaaa
     7861 cgtaattatt tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca
     7921 acaatgcact atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg
     7981 cacaagcgca aaaaccgcgc tttctatgga ttggatgttc cgacagtcgc gttcctgcag
     8041 aacgtttaac cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg
     8101 tcattcacac cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactagaag
     8161 ttgaacacat tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa
     8221 acccggaact ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac
     8281 atagctcatt gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga
     8341 acgtcatgga acaggtgtat aacctgggcc actccaccat tatgcaatca gcgtggaaac
     8401 gcgggcagaa agttaccatt cacggctggg cctatggcat tcacgacggc ttgctgcgtg
     8461 atctggatgt cacagcgact aaccgcgaaa cccttgagca acgttaccgt cacgggattt
     8521 ccaacctcaa gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat
     8581 ccggcaactt cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg
     8641 gtaacgctgt gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc
     8701 gataaattct accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat
     8761 cgccagcgac ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc
     8821 cgagtcgatg atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc
     8881 tttgaggatt ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat
     8941 aaagtcgact tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga
     9001 gccacgcagc agaccaacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat
     9061 ctgacgaccc agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac
     9121 agtatgtttc atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt
     9181 ttggcaccgc aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa
     9241 tctgttaata aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg
     9301 ctactaaaat attaatgaat tgaaatggtg tctctttatg gcaattaaca atacaggctc
     9361 gcgacgatta ctcgtcacgc taacagccct ttttgcagcg ctttgcgggc tgtatctact
     9421 cattggcgga ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggtct
     9481 tgtgatgctc ggcgtcgcct ggatgctgtg gctcagtaaa cgtgccgcgc tttggctgta
     9541 cgcagccctg ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg
     9601 ggcgctgact ccgcgcagcg acattctggt cttcttcggc atctggctga tcctgccgtt
     9661 tgtctggcgt cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtggcact
     9721 gctgattagc ggtggtatcc tgacctgggc cggatttaac gatccgcagg aaatcaacgg
     9781 caccttaagc gccgatgcca cacctgccga agcaatctcc cccgttgccg atcaggactg
     9841 gcctgcctat ggtcgtaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc
     9901 cgataacgtt cacaagctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc
     9961 gaacgatccg ggtgaaatca ccaatgaagt gacgccgatt aaagtgggcg acacccttta
    10021 cctgtgtacc gctcaccagc gactgtttgc gctcgatgcc gccagcggca aagagaaatg
    10081 gcattacgat cctgagctga aaaccaacga gtctttccag cacgtaacct gccgtggcgt
    10141 ctcttatcat gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg
    10201 tcgtatcatt cttccagtga atgatggtcg cctgattgcg attaacgctg aaaacggcaa
    10261 actgtgcgaa accttcgcca ataaaggcgt gcttaatctg caaagcaata tgccagacac
    10321 caaaccgggt ctgtatgaac cgacttcgcc accgattatc accgataaaa ccatcgtgat
    10381 ggcaggttca gttaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt
    10441 tgatgtcaac accggggagc tgctgtgggc ttttgatccg ggcgcgaaaa atccgaacgc
    10501 aatcccgtct gacgaacaca cctttacctt taactcgcca aactcgtggg caccagcggc
    10561 ctatgacgcg aagctggatc tggtgtatct gccgatgggc gtgaccacgc cggatatctg
    10621 gggcggtaac cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc
    10681 cactaccggg aaactggcgt ggagctacca gaccgttcac cacgacctgt gggatatgga
    10741 tcttccggca cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccagttat
    10801 ttacgctccg gcgaaaaccg gcaacatttt tgtgctcgat cgtcgtaatg gcgaactggt
    10861 ggttccggca ccggaaaaac cggttcccca aggtgctgcc aaaggcgatt acgttacccc
    10921 tactcaacca ttctcggaac tgagcttccg tccgacgaaa gatttgagcg gtgcggatat
    10981 gtggggagcc accatgtttg accaactggt gtgccgcgtg atgttccacc agatgcgcta
    11041 tgaaggcatt ttcaccccgc catctgaaca gggtacgctg gtcttcccgg gtaacctggg
    11101 gatgttcgaa tggggcggga tttccgttga tccaaatcgt gaagtggcga ttgccaaccc
    11161 aatggcactg ccgtttgttt cgaaactgat cccgcgtggt cctggtaacc cgatggagca
    11221 gccgaaagat gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc
    11281 gtatggtgtc acgctcaacc cgttcctctc accgtttggt ctgccatgta aacagccagc
    11341 atggggttat atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat
    11401 tggtacgccg caggacagta tgccgttccc gatgcctgta ccggtgccgt tcaatatggg
    11461 tatgccgatg ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgccgctac
    11521 ggcagataac tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcaaggtcg
    11581 tttaccagcg ggtggtcagg cgacgccaat gacctatgaa gtgaatggta agcagtatgt
    11641 ggtgatctcc gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc
    11701 ttatgccctg ccggatgatg tgaagtaaga cttgctcaga ttgctgacaa cgtgcgcgtt
    11761 gttcatgccg gatgcggcgt taacgcctta tccagcctac aaaattgtgc aaaatcaatg
    11821 gattgcacag gtagcgtagg cctgataagc gtagcgcatc aggcagattt gcgtttgtca
    11881 tcagtttaat gcccggagag atccgggcat atttccgaat acggtctttt tataccgtaa
    11941 accctaacat catccccgta tcttcatgct ccagcagatg gcagtgcgcc atataagcat
    12001 gttctttcgg tgcatcgtga ttaaacttca ccagcacttc gctgacatta ccttctacct
    12061 taacggtatc tttccagccc gcgcgatgag ccgctggcgg tttgccattt tctgacaaga
    12121 tacggaactg cgtgccgtga atatggaacg gatgcagcat catgtcaccc acgccagaga
    12181 taacccaacg ttcgtactgc cctttcgccg ccgcaaacat cggcttattc atatcaaacg
    12241 cctgaccgtt gattttattg gcatggtgga aatcgaactt cccgccgtgg ttcatatgat
    12301 tcatattgcc gtgccccata tggcccatca tctggctgtg atccatcccg gccatcgcct
    12361 gatcgccata tttctccatc agcatctgca tacccatcat atcgagcatc gggtccatag
    12421 aaagttgcag cttgcgtacc gtcagccctt ccagcgaagg taacgcaggc aggctactta
    12481 atgtgtctgg caaagcaccg gaggcactaa tagcaatcgg ctgaatccgc attaccggat
    12541 gcggcttgtc aaacggcgca atcgccatcc ccatctggct gaccggcagc gtcaccaggt
    12601 caaagggttt gttgtcgtta acctccacca gcacttcaaa acgctcgccc atcagcaccg
    12661 gcagctcgtt caccttcacc ggttcaggta gcagaccacc gtcgctggca atcacataaa
    12721 gcgggcgatt gtcgctggtg gcgaaattga gcgagcgggc attacagcca ttgagcaaac
    12781 gcaggcgcag ccaaccacgc ggggcagcgt gttgcgggta gattgcaccg ttggtcagca
    12841 acgtatcgcc aaaccagccc acggcggcgg tcatcacatc cagttgataa tcaatctgcc
    12901 cgtcggcgct aaatttctta tcctgaacga tcaccggaac atcatcgata ccccactgtt
    12961 ttggcagcat taatttcagg atctcgtcat cttcaattac caccaaccca gccaacccca
    13021 tcgccacctg tcgcccggtc ttgccatgtt gatgtggatg gaaccagcag gtagcggcag
    13081 gttgatcaac gttcaacgtc accgagcgct tgccacctgg cggaataatt ccctgcgggc
    13141 cgccgtccac ttcacccggt acttccagcc cgtgccagtg caacgtcgtc tcttccgtca
    13201 gttggttata gatatcaacc gttaccgctt tgccgtgctg taatttcacc gccggcccca
    13261 gcagattgcc gttatagccc caggtagttg cagttttccc gccaaaggtg gactggcctg
    13321 cgccaatagt taactgaatg cgattacggg catcggtcgt gagcaaatca gggatcggta
    13381 acgttgggcg ttccgccgca aagactgcgc ggctccacag cggcaaggct gaagccacac
    13441 ccagcgcgac ggaatatttt aagaaatcac ggcgttgcat agttatttcc ttattcttaa
    13501 gcaggcgaaa caaaatcaaa cgttgagcat agtccttccc cttacgggaa ggtcaagccg
    13561 cagacacaat ttaatcgaca ataatggtcg tcgcctcgct cgcagtgtgc taacgtttat
    13621 cttctttaag cccctggtag aagcaatgaa gacgtttttc agaacagtgt tattcggcag
    13681 cctgatggcc gtctgcgcaa acagttacgc gctcagcgag tctgaagccg aagatatggc
    13741 cgatttaacg gcagtttttg tctttctgaa gaacgattgt ggttaccaga acttacctaa
    13801 cgggcaaatt cgtcgcgcac tggtcttttt cgcccagcaa aaccagtggg atctcagtaa
    13861 ttacgacacc ttcgacatga aagccctcgg tgaagacagc taccgcgatc tcagcggcat
    13921 tggcattccc gtcgctaaaa agtgcaaagc cctggctcgc gattccttaa gcctgcttgc
    13981 ctacgtcaaa taatccctga tacctttttg tagaaatatt gaccgtgcat ccgcggtcaa
    14041 tgttagctat tatgttgcgc ccttttttta cgggtgttaa caaaggaggt atcaacccat
    14101 ggccgaaaaa aaacagtggc atgaaacgct acacgaccag tttgggcagt actttgcggt
    14161 agataacgtt ctgtatcatg aaaagaccga tcaccaggat ctgatcattt ttgagaacgc
    14221 tgcatttggt cgcgtaatgg cgctggatgg cgtagtacaa accaccgagc gcgacgagtt
    14281 tatctatcat gagatgatga ctcatgttcc gctactgacc cacggtcacg cgaaacatgt
    14341 gctgattatc ggcggcggcg acggtgccat gctgcgtgaa gtaacccgac ataaaaacgt
    14401 tgagtcaatc acgatggtgg aaatcgatgc gggtgtcgta tcgttctgcc gtcagtatct
    14461 acccaaccat aacgccggta gctacgacga tccgcgcttt aagctggtga tcgatgatgg
    14521 cgtcaatttc gttaatcaaa ccagccagac ctttgatgtc attatctccg actgcaccga
    14581 tcctatcggt cccggcgaaa gtctgttcac ctcagcattt tatgaaggct gcaaacgttg
    14641 cctgaatcct ggcggtatct tcgtcgcaca aaacggcgtc tgctttttac agcaggaaga
    14701 agccatcgac agccatcgca aactcagcca ttacttcagc gacgttggct tttatcaggc
    14761 ggcgatcccg acctattacg gcggtatcat gacttttgca tgggcgacag ataacgacgc
    14821 cttacgccat ctctcaaccg aaattattca ggcgcgtttt ctcgcttctg gcctgaaatg
    14881 ccgttattac aatccggcaa tccatacggc agcttttgcc ttacctcagt atctgcaaga
    14941 cgcactggct tcacagccgt cctaaggaga agataagaaa ttgaaaaaac tgaaactgca
    15001 tggctttaat aatctgacca aaagtctgag tttttgtatt tacgatatct gctacgccaa
    15061 aactgccgaa gagcgcgacg gttatattgc ttatatcgat gaactctata atgccaaccg
    15121 tctgaccgaa atcctgtcag aaacctgttc cattatcggg gccaatattc ttaacatcgc
    15181 ccgtcaggat tacgaaccac agggtgccag cgtcactatt ctggtgagtg aagaaccggt
    15241 cgacccgaaa cttatcgaca aaacagaaca ccccggccca ctgccagaaa cggtggttgc
    15301 ccatctcgat aaaagtcata tttgcgtaca tacctacccg gaaagtcatc ctgaaggcgg
    15361 tttatgtacc ttccgcgccg atattgaagt ctctacctgc ggcgtgattt ctccgctgaa
    15421 ggcgctgaat tacctgatcc accagcttga gtccgatatc gtaaccattg attatcgcgt
    15481 gcgcggtttt acccgcgaca ttaacggtat gaagcacttt atcgaccatg agattaattc
    15541 gattcagaac tttatgtctg acgatatgaa ggcgctgtat gacatggtgg atgtgaacgt
    15601 ctatcaggaa aatatcttcc ataccaagat gttgcttaaa gagttcgacc ttaagcacta
    15661 catgttccac accaaaccgg aagatttaac cgacagcgag cgccaggaaa ttaccgctgc
    15721 gctgtggaaa gaaatgcgcg agatttatta cgggcgcaat atgccagctg tttaacggct
    15781 ctggcggagc tcccaggctc cgccagattt atttacttct gctgcacgaa attgcggtaa
    15841 gccgccacga cctgcagaaa atcctcaacg ccgcatagcg acaggctttc ttcgtcgtag
    15901 tagttcatcc cttcttccat ttcatcgcca gcgaattcca gttgattggc gcgaaccatc
    15961 acctcttcac cgtccatcca cagggtgtat tcatgccctg cccgttgcca ggaccgttcg
    16021 ctacctttca gtgcgtgcgc tgcttgttcc acttcatcaa gcaaagccag gttttctttc
    16081 acctcttcat taaaccagtg cccgaccact tcatgcccca tggacatacg cacctttacc
    16141 actccggtaa tatcgcgcag aaattcgtaa tccatagtgt gttcctctgc tcccggcaat
    16201 gcgccgaaat cgcattgcgc cattgatgta attatcgcaa ccttaccgga gaaaaacagc
    16261 ggaacggcga tggtttagaa ataaaaaatg cccgggaatc tcattccccg agcattgtgt
    16321 cgtttatgcg cagcgcgtgc gctgactttt taaaccgcag tctggaaaat caccccatcg
    16381 gctttttcgg tatactgaga aagctggttg aagttcagat aacgataagt atcaacggca
    16441 gttttatcca cttgcgccac gtaggtctgg tactcttccg gcgtcggcag tttgccaatc
    16501 agcgccgcaa cagccgccag ttccgcagaa gccaggaaga cattcgcgcc agtacccaga
    16561 cggttcggga agttacgggt agaggtggaa accaccgtcg caccgtctgc tacacgcgcc
    16621 tggttaccca tacacaggga acagccaggg atctcgatac gcgcaccact cttaccgaag
    16681 acgctgtagt agccttcttc agtcaactgc gcggcgtcca tacgggttgg cggtgccacc
    16741 cacaggcggg tcggcagttg gcctttgtgc gcatccagca gtttacccgc agcacggaag
    16801 tgaccgatgt tggtcatgca ggaaccgata aatacttcgt cgatcttctc accctgtacc
    16861 gcagacagcg gacgcgcgtc atccgggtcg ttcggtgcac agaggattgg ctctttaata
    16921 tccgccagat cgatgtcgat cactgccgcg tattcagcat ctgcatcggc ttccagcagc
    16981 tcaggattcg ccagccattt ttccatgccc tgaatacgac gttccagggt gcgacgatcg
    17041 ccgtaacctt ccgcgatcat ccacttcagc aggacgatgt tagagttcag gtattcgatg
    17101 atcggttctt tgttcagctt gatggtacaa ccagcggcag aacgctcagc ggacgcatcg
    17161 gtaagctcaa aggcctgctc aactttcaga tccggcagac cttcaatttc cagaatgcgg
    17221 ccagagaaga tgtttttctt gcctttcttc tcaacggtca gcagaccttg tttgatggca
    17281 tacagcggaa tggcgtgtac cagatcgcgc agggtgatgc ccggctgcat tttgcctttg
    17341 aagcgcacca gaacggattc cggcatatcc agcggcatta cgcctgttgc ggctgcaaat
    17401 gccaccagac cagaacccgc cgggaaagag ataccgatcg ggaaacgggt atgggagtca
    17461 cctccggtac cgacggtatc cggcagcagc atacggttca gccaggagtg gattacgccg
    17521 tcacccggac gcagcgacac accgccacgg ttcataatga agtccggcag cgtgtggtgc
    17581 gtgttcacgt caactggctt cggatacgcc gcggtgtggc agaaagactg catcaccagg
    17641 tcagccgaga agcccaggca cgccaggtct ttcagttcat cacgggtcat cgggccggtg
    17701 gtgtcctgag agcctacaga agtcattttc ggttcgcagt acgcgcccgg acgaatgcct
    17761 ttcacgccac aggcacggcc taccattttc tgcgccagcg agaagccgcg atcgctctca
    17821 gcgacatctt tcgcctgacg gaacacatca ctgtgcggca gaccaagtgc ttcacgcgct
    17881 ttggtggtca ggccacgccc gataatcagc ggaatacggc caccagcacg cacttcatca
    17941 atcagcacgt cggttttcag ttcgaaggtc gccagcagtt cgccggtttc gtggttacgc
    18001 acttcacctt tgtacgggta aacgtcaatc acgtcgccca tgttcaggtt agagacgtcg
    18061 acttcgattg gcagtgcacc cgcgtcttcc atcgtgttaa agaagatggg tgcaatttta
    18121 ccgccgaggc acaaaccacc gccgcgtttg ttcggcacat gtggaatatc atcgcccata
    18181 aaccacagaa cggagttagt ggcggattta cgcgaagaac ccgtacccac aacgtcaccg
    18241 acgtacgcca gcgggaaacc tttctgttgc agagcttcga tttgcttgat cggaccaaca
    18301 acaccaggct ggtctggctc aataccttca cgggcgtttt tcagcatcgc cagcgcgtgc
    18361 agtgggatat ccgggcgtga ccacgcatcc ggtgccggag aaaggtcatc ggtgttagtt
    18421 tcgccagtga ctttgaagac agtaacggtc agtttttcag ccagcgccgg gcgattcagg
    18481 aaccattcgg catccgccca ggactgcata acctgcttcg catattcgtt gcctgctttc
    18541 gctttctctt ctacgtcata gaagttatcg aacatcagca gcgtgtgaga aagtgctttg
    18601 gcagcaatag gtgccagttt ggcatcatcc agcgcgtcga tcagcggatg aatgttgtaa
    18661 ccaccctgca tggtgcccag cagttcgatg gctttttccg gagtcagcag aggggatttg
    18721 gcttcgcctt tcgcgatagc agccaggaag cctgctttga cataggcggc ttcatcgacg
    18781 cctgggggaa cacggttggt taacagatct aacaggaatt cttcttcgcc cgcgggcggg
    18841 tttttcagca gctctacaag tgcggccatt tggtttgcat ccaggggttt gggcgcaatc
    18901 ccctcagcgg cacgctcagc tacgtgctta cggtattctt ctagcacgac ggttctcctc
    18961 gctctcattg tcatagtgcg gcaggcgatt ctcttcacgc tcctgtgaga cagcagtttg
    19021 tagggtaaat gcccagtacc gcaacgggca gaatagcagg attttgacga tgtgttaatc
    19081 tgtttacaaa aaagcaacat aaaaaagtgg ctgaatcgtt aaggatggtc tagagattgt
    19141 ttccctccgt aaaaacttcg ttaattcgca tggtgataat cacctttctc aacgaatccc
    19201 aagattagtc aaaatttaaa ctaccgcctc tttatactcg gattcacagc acctgcgggt
    19261 ggcagttcgc cgaccattgc gatttccttg agatccgaat tatgaaaatg actttgccgt
    19321 ttaaacccca tgtgctggcg ctaatttgca gtgccgggct ttgtgccgcc tctaccgggc
    19381 tatatataaa aagccgcaca gtggaagcgc ctgtggaacc gcaatcgaca caacagactg
    19441 cgcctgacat caccgcagtt acgcttcctg caacggtttc cgcaccgccc gtaacgcccg
    19501 ccgtcgtcaa atccgcattc agcactgcac aaatcgatca atgggttgcg cctgtcgcgc
    19561 tgtaccccga ttctctgctt tcacaagtgt taatggcatc aacctatccg gcaaacgttg
    19621 ctcaagcagt gcaatggtcg cacgataatc cacttaaaca aggcgatgct gctattcagg
    19681 cggtatctga ccagccgtgg gacgccagcg ttaaatcact ggtggccttt ccacaattga
    19741 tggcattgat gggcgaaaac ccgcaatggg tgcaaaacct gggcgatgct tttctggcgc
    19801 agccgcagga cgtgatggac tcggtacagc gattgcggca actggcgcaa caaaccggtt
    19861 cgctgaagtc atcaaccgaa caaaaagtta ttaccacaac gaagaaagct gtaccggtaa
    19921 aacagacagt cacggcaccc gtcataccat ccaataccgt tttaactgcc agccccgtca
    19981 ttacagagcc tgcaacaacc gtcatttcca ttgagcccgc caatcctgat gtggtctata
    20041 ttcccaacta caacccaacc gtggtttacg ggaactgggc caatactgcg tatccgccgg
    20101 tttatctgcc accaccagcc ggagaaccgt ttattgacag ctttgtgcgc ggattcggct
    20161 atagcatggg tgttgctacc acgtacgcac tattcagcag catcgactgg gatgacgacg
    20221 atcatgacca tcatcatcgt gacggtaatg gctggcaaca caacggcgac aacatcaata
    20281 tcgacgtcaa caatttcaac cgtatcaccg gtgagcatct tactgataag aatatggcat
    20341 ggcggcacaa tccaaactac cgtaatggtg tgccctatca tgatcaggat atggcaaagc
    20401 ggtttcatca aaccgatgtc aacggcggaa tgagcgccac gcagctacct gctccaacac
    20461 gcgacagcca gcgtcaggcg gcagcaagtc agtttcagca acgaacacac gccgcccccg
    20521 tcattacacg agatacccaa cgtcaggcag cggcacagcg gtttaatgaa gctgaaaact
    20581 atgggagcta tgacgacttc cgcgacttca gccgtcgcca accactgacc cagcaacaaa
    20641 aggacgccgc tcgtcagcgt tatcagtcgg cctcgcctga gcagcgccag gcagtccgcg
    20701 agaaaatgca gactaaccca cagatccagc agcgaagaga cgcagcgcgt gagcgtattc
    20761 agtccgcctc gcctgagcag cgccaggcag tccgcgagaa aatgcagact aacccacaga
    20821 tccagcagcg aagagacgca gcgcgtgagc gtattcagtc agcctcgcct gagcagcgcc
    20881 aggtgtttaa ggaaaaagta cagcagcgcc cactgaacca acagcaacgt gataacgccc
    20941 gccagcgtgt tcaatcagca tcacctgaac aacgtcaggt ttttcgggaa aaagttcagg
    21001 agagccgccc acaacgtcta aacgacagta accatactgt caggctgaat aacgagcaac
    21061 ggtcagcagt acgcgaacgt ctctctgagc gcggagcaag gcgacaggaa aggtaaattg
    21121 cagacgtaaa aaaagcggcg tggttagccg cttttttaat tgccggatgt tccggcaaac
    21181 gaacaattac ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac
    21241 ttctgccgcc aggcccacag actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat
    21301 gtcttcagca tcacaaccca tttcgattgc caggccgatt tcacccagca gctcgccgcc
    21361 gttggtaccg acaatcgcac caccgatcac acggtgagat tctttgtcga aaatcagctt
    21421 ggtcataccg tctgcgcagt cggaagcgat agcacgacca gaagcagccc acgggaaggt
    21481 ggcggtttca tagctgatgc ctttctcttt cgcttctttc tcagtcagac ctacccatgc
    21541 aacttctggt tcggtatagg cgatggacgg gataactttc ggatcgaagt agtgtttctt
    21601 accggcgata acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg
    21661 ctgaccgacg atatcgccga tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc
    21721 aacgcggatg aaaccacggt cgtccacttc cacgccagct ttgcctgcgt cgaggttttt
    21781 accgttcggc acacgaccaa tcgctaccag cacggcgtcg taacgctgcg gttcagcggg
    21841 tgcttttttg ccttccatcg tcacataaat accgtcttct ttcgcttcaa cggcggtaac
    21901 tttggtttcc agcatcaggt tgaatttctt gctgatacgc ttggtgaaga ctttaacgat
    21961 gtctttgtca gctgccggga taacctggtc gaacatttca accacgtcaa tctgtgaacc
    22021 cagcgcgtgg tatacggtgc ccatttccag accgatgata ccgccaccca ttaccagcag
    22081 gcgttctggt acttctttca gttccagcgc gtcagtggag tcccagatac gcggatcttc
    22141 atgcggaata aacggcagtt ggatcgggcg agaacccgct gcaatgatcg cgttgtcgaa
    22201 gttgatcacg gttttaccgt tctcaccttc aacttccagg gtgttagccc cggtaaattt
    22261 acccagaccg ttgaccactt tgactttgcg gcctttcgcc ataccagcca gaccaccggt
    22321 cagctgattg atcactttct ctttccaggt acgaatcttg tcaatatcgg ttttcggttc
    22381 gccgaagacg ataccgtgtt cagccagcgc tttggcttct tcgataactt ttgctacgtg
    22441 cagcagtgct ttagaaggga tacagccgac gttcaggcaa acaccgccaa gggtgttgta
    22501 acgttctacg attacggttt ccagacctaa atcagcgcaa cggaaggcag cggagtaacc
    22561 tgcggggcct gccccaagta ccacgacctg agttttgatt tcagtactca tcatgacctc
    22621 tatatattta tctccggcgg gtctgacgtt ttttttatga cgcccattat ccaccgggtc
    22681 gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa acggcaaccg
    22741 atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa aagccggccg
    22801 ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg tgttgttaat
    22861 gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga aggagagaga
    22921 aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct ccatcgcgga
    22981 cttggaaacg ccgaggatag ccacttccgg cgcgttcaca atcggcgcga agtgggtagt
    23041 acccaggccg ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc ccgcagtcag
    23101 cttaccgtca cgcgctttct tagaaatagt catcagctcg cgagacagct cgatgatgcc
    23161 tttcttgttg acgtctttga ataccggaac aaccagaccg ttcggggtat ccaccgccac
    23221 accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca gcgaactatt
    23281 gaagcgaggc atctgctcaa gagctgcagc aacggctttc atgatgaaga caaccggggt
    23341 gatcttcaca tccagcttac gtttcgccgc ttcttcgttc tgctgtttac ggaacgcttc
    23401 cagctcggtg atatcggttt tgtcgaagtg agtaacatgc gggatcatca cccagttacg
    23461 gctcaggttc gcaccagaaa ttttctggat acggcccagt tccacttctt cgatttcacc
    23521 aaacttgctg aagtccacct tcggccacgg cagcatgcct gggataccgc cgccagtcgc
    23581 cgccggagct gcttctgcac gtttgatagc ttctttcacg taagcctgaa cgtcttcgcg
    23641 caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc caaactcgcg
    23701 tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg tcgttttcag caaattcaga
    23761 tttgccttcg gctttcgcag ccggagctgc tgccggagct tcagcttttg ctgccggagc
    23821 cggcgctgcc gcttcctgtt tcgccggagc cgccgcaggc gctgcgcctt caacttcgaa
    23881 gatcataatc agcgagccag ttttcacttt atcgccaacg ttgactttca gttccttcac
    23941 gacgcctgca aacggagccg gaacttccat agaagctttg tcgccttcta cggtaatcag
    24001 tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca cttcaacttc
    24061 gtcaccgccg atatccggaa cgttaacttc tttcacgcca gccgctggtg caggggccgc
    24121 tgccggagcc gcttcctgtt tagccgccgg agctgccgcg cctgcttcac ccgcgacttc
    24181 gaagaccata atcagcgagc cggtagacac tttgtcaccc acgttcactt tgatctcttt
    24241 cacggtgcca gcaaacggag ccggaacttc catagaagct ttatcgcctt ctacggtgat
    24301 cagcgactgt tcagcttcaa ctttatcgcc aactttcacc aggatttcgg tcacttcaac
    24361 ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gccgcagccg cgggtgctgc
    24421 tgccggagct gcttctttct tctcttctgc ctgagcaggt gcagcgtctg ctgcaccgtc
    24481 ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa cagagacttt
    24541 gatctcttta acgatacccg cctgcggaga cggaacttcc atagaggctt tgtcgccttc
    24601 tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca ggatctcggt
    24661 gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca ttattctttt
    24721 acctcttacg ccagacgcgg gttaacttta tctgcatcga tgttgaattt ggcgattgcg
    24781 tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag cgccgcaacc
    24841 acgacataag aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct gtcggaacga
    24901 ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt acggacctgc
    24961 tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcgtcgtt catcacctga
    25021 gcgatatacg gaacgcgcgg agtttccagc ggatgaagca tgttccagcg ttcacaatcc
    25081 tgaccatcac gcgccagctc ggtgaaggag gtcacgctat aaacgtcaga acctacgccg
    25141 taatctttcg ccaggatctc tgccgcttca cggacgtgac gcaggataga accggagccc
    25201 agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat acctttacgg
    25261 ataccttcct cagcaccttc cggcattgcc ggcatgtggt agttttcgtt cagcgtagtg
    25321 atgtagtagt aaacgttctc ttgtttttca ccgtacatac gctccagacc gtcatgcatg
    25381 atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg gatagtcagc
    25441 gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt caccgttcag ggtggtacga
    25501 ccggaagtac cgccgatcag gaagccacgc gcttgctggt cgccagccgc ccagcacaga
    25561 tcgccgatac gctggaagcc gaacatcgag taatagatgt agaacgggat catcggcaga
    25621 ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc cagctcgttg
    25681 atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac ctgctcgcgg
    25741 tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg gaacagacct
    25801 tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc tttgatcgac
    25861 ttgttcttca gcattacgtt cagggcacga acgaaagcga tagtggtaga gatctctttg
    25921 ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag cttctcggtg
    25981 aagttcggct gacggcttgg cagataaccg tgcagtttct gacgctgtgc gtgcagatag
    26041 gtatgctctt cagaaccttc cgggaaggtg atgtacggca gtttttcgat atctgcatca
    26101 gacaccggca cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt cattttctta
    26161 acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc tttaatggta
    26221 tgagcaagga ttactgtcgc tttgcctttg gtttcctgcg ctttcttgaa tgcagcgtag
    26281 attttcttcg gatcgtgacc accacggttc agtgcccaga tctgctcgtc agtccagtct
    26341 gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcacgaac gtacgcacca
    26401 tctttcgatt tgaaggtttg gtagtcgccg tcaacggttt cgttcatcag ctggatcagt
    26461 ttaccgctgg tatctttacg cagcagttca tcccaacggc taccccacat cactttgatc
    26521 acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt gccgttaccg
    26581 gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaagaccag gttatccagt
    26641 ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat ttcaccgtcg
    26701 ccgaggaacg cgtaaacggt ctgtttagag gtatctttca ggccacggtg ttccagatat
    26761 ttcaggaatt tagcctggta aatagcacca atcggcccca gacccataga tacggtcggg
    26821 aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc attgccgtga
    26881 acttcctgac ggaagttatc cagctgctcc tgagtcagac gaccttccag gaaagcacga
    26941 gcgtacacgc ccggggagat gtggccctgg aagtaaacca ggtcgccgcc atcctgctcg
    27001 ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga agactggaag
    27061 gacgccatat ggccgcccag ttcgaggtct tttttcgacg cacgcagcac cgtcatgatg
    27121 gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc cggatactcc
    27181 ggttgttctt caacggggat ggtgttgatg tagttgctga tacctgtgcc tgcggctacg
    27241 tttacaccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg agcacgctca
    27301 acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt ttcgatcgga
    27361 tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta ataacgttga
    27421 gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac aggttctacg
    27481 tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata atttttacca
    27541 gaaaaatcac taattctttc gttgctccag acgacgcaga gaacgctcac ggcggctctc
    27601 ttcacgactt ctgtcgagca aaatttcttc gataaaggcc agatggcgat gcgatgcttc
    27661 gcgcgcttct tccggcttac cggccataat cgcttcaaat atgcgggtgc ggtgactact
    27721 caccagcggc agcatctcgc gacgcgaata gagcaattcg aagttctggc ggacattctg
    27781 ggccaacatc ggctccatac accttagcag atgaagcaga accacattgt gggccgcttc
    27841 ggtgacggca atctgatact ggagtacggc gtttgattcc gcgtccagat cgccagactg
    27901 ctgcgccagc tctatggcgt ggtggagttc acggatgcgt tccttgtctt catcggtact
    27961 acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt cgagcaagtc
    28021 atactgtgac tcaggatggt cggagagcag ctccaccagc ggatcgctga agctttgcca
    28081 taggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc ccttcgcttc
    28141 gagacgttga atcgcctcac gcaaggaggg acgggagacg tcaaactgtt ttgccagttc
    28201 gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca aaaactccag
    28261 ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg ccatgagttc
    28321 ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct gtgacgctaa
    28381 agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg aaactgtgca
    28441 cattttaaca acttgacata tataacgttt caaagttgta actatgcaca aatgtagact
    28501 ttacg
//
LOCUS       DAPHJE010000056        28217 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPHJE010000056 DAPHJE010000000
VERSION     DAPHJE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.056"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="RH222_002432"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="RH222_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3797434.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="RH222_002433"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="RH222_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV3797435.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="RH222_002434"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="RH222_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV3797436.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="RH222_002435"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="RH222_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV3797437.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="RH222_002436"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="RH222_002436"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV3797438.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="RH222_002437"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="RH222_002437"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV3797439.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="RH222_002438"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="RH222_002438"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV3797440.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="RH222_002439"
     CDS             complement(4523..5245)
                     /locus_tag="RH222_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3797441.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="RH222_002440"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="RH222_002440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3797442.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="RH222_002441"
     CDS             6445..6663
                     /locus_tag="RH222_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV3797443.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="RH222_002442"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="RH222_002442"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV3797444.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="RH222_002443"
     CDS             complement(7397..7597)
                     /locus_tag="RH222_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV3797445.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="RH222_002444"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="RH222_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV3797446.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="RH222_002445"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="RH222_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV3797447.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10094..12007
                     /locus_tag="RH222_002446"
     CDS             10094..12007
                     /locus_tag="RH222_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3797448.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12007..13029
                     /locus_tag="RH222_002447"
     CDS             12007..13029
                     /locus_tag="RH222_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3797449.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13023..13241
                     /locus_tag="RH222_002448"
     CDS             13023..13241
                     /locus_tag="RH222_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV3797450.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13295..14164
                     /locus_tag="RH222_002449"
     CDS             13295..14164
                     /locus_tag="RH222_002449"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV3797451.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14219..14623)
                     /locus_tag="RH222_002450"
     CDS             complement(14219..14623)
                     /locus_tag="RH222_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV3797452.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14925..15557
                     /gene="crp"
                     /locus_tag="RH222_002451"
     CDS             14925..15557
                     /gene="crp"
                     /locus_tag="RH222_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV3797453.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15608..17698
                     /locus_tag="RH222_002452"
     CDS             15608..17698
                     /locus_tag="RH222_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3797454.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFAACSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="RH222_002453"
     CDS             complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="RH222_002453"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV3797455.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="RH222_002454"
     CDS             complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="RH222_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV3797456.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19666..20268)
                     /locus_tag="RH222_002455"
     CDS             complement(19666..20268)
                     /locus_tag="RH222_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV3797457.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20258..20425)
                     /locus_tag="RH222_002456"
     CDS             complement(20258..20425)
                     /locus_tag="RH222_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV3797458.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="RH222_002457"
                     /gene_synonym="rotA"
     CDS             complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="RH222_002457"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV3797459.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21373..22554
                     /gene="tsgA"
                     /locus_tag="RH222_002458"
     CDS             21373..22554
                     /gene="tsgA"
                     /locus_tag="RH222_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV3797460.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22816..25359
                     /gene="nirB"
                     /locus_tag="RH222_002459"
     CDS             22816..25359
                     /gene="nirB"
                     /locus_tag="RH222_002459"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV3797461.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25356..25682
                     /gene="nirD"
                     /locus_tag="RH222_002460"
     CDS             25356..25682
                     /gene="nirD"
                     /locus_tag="RH222_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV3797462.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25810..26616
                     /gene="nirC"
                     /locus_tag="RH222_002461"
     CDS             25810..26616
                     /gene="nirC"
                     /locus_tag="RH222_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV3797463.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26635..28008
                     /gene="cobA"
                     /locus_tag="RH222_002462"
     CDS             26635..28008
                     /gene="cobA"
                     /locus_tag="RH222_002462"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV3797464.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6825 a         7566 c         7129 g         6697 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcac ttgccggtga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaactcatg gccttctact
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg cttttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcgtcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat ttacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacgtccaac agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag agagcggttc accacgagtg taagagttgt cgaactcttt accgtcgatc
     5701 agagtacctt tgtagttcac gacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc
     6061 gaggcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaaacacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ctttttcggt attagtggca
     6721 accgcaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtggcca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtggt gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca cgacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgctcgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccgtat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acaacgccat ctggtcgccc tggaataagc gttgtgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc accaccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacagca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gaggttgagt gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaatgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaagcggaa taccggacgc agtaaatagc
     8821 gcccaccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aatgttcgtc tgccgacccc gccaacaacg gcactaacgc cagtgctggg attactgcca
     8941 gatcctgaaa cagcagaacc gaaaacccga gctggccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaat gccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagtttgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaatac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatac ccagccgcga tgccagcggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag cacatcgctc atcgggtagc gattcagcgc
     9601 gtcataacgg taagcacttt ctggctcgcc ggtggtaatc acgctacgcc agtactttcc
     9661 cgccagttgg tttcctcccg gcccgctggc aaaaccacga cttaataccc ggtccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgctgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc ggtttaagca gtacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tatcataatg ataagttaac atagtctgaa
    10081 catacggcac cttatgattg ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct
    10141 gctggataat gccaccgcca ccatcaaccc agggcagaaa gtcggcctgg tgggtaaaaa
    10201 cggctgtggt aaatctaccc tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg
    10261 cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt
    10321 accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc aactagaagc
    10381 gcagctacac gacgccaacg aacgtaacga cgggcacgcc attgcgacca ttcatggcaa
    10441 gctggatgct attgacgcat ggagtattcg ctcccgtgct gccagcctgc tgcacggcct
    10501 cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttttccgggg gctggcgtat
    10561 gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc
    10621 gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggttga agagctatca
    10681 gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg ttgataaaat
    10741 tattcatatc gaacaacaaa gcatgttcga gtacaccggt aactacagtt cgtttgaagt
    10801 acagcgcgcc acccgtctgg cgcagcaaca agcgatgtat gaaagccagc aggaacgcgt
    10861 agcgcatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc
    10921 ccagagccgc attaagatgc tcgagcgtat ggagctgatt gcccccgcgc acgtcgacaa
    10981 cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac tgaagatgga
    11041 aaaagtcagc gcgggctatg gcgatcgcat tattctcgac tcgattaaac tgaacctggt
    11101 gcccggctcg cgcattggtc tgttaggccg caacggcgcg ggtaaatcga cattaatcaa
    11161 actgttagcc ggtgaacttg cgccagtcag cggtgaaatt ggtctggcga aagggatcaa
    11221 gctcggctac ttcgcccagc atcaacttga atacctgcgc gccgacgaat cacctattca
    11281 acatctggca cgtttagcgc cgcaggagct ggaacaaaaa ctgcgtgact acctcggcgg
    11341 ctttggtttc cagggcgata aagtaaccga agaaacgcgc cgcttctcag gtggggaaaa
    11401 agcccgcctg gtgctggcat taattgtctg gcagcgtccg aatctgctgc tgctcgacga
    11461 accgactaac caccttgacc tcgacatgcg tcaggcactc accgaagcat taatcgagtt
    11521 cgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg ctgcgttcca ccactgacga
    11581 tctctacctg gttcacgatc gtaaagtcga accgttcgac ggcgatctgg aagattatca
    11641 acagtggttg agcgacgtac aaaagcagga aaaccaggcc gacgaagcgc caaaagagaa
    11701 cgcgaacagc gcccaggcac gtaaagatca gaagcgtcgg gaagcggagt tgcgtgcgca
    11761 aacccagcca ctgcgtaaag agattgcccg tctggaaaaa gagatggaga agctgaacgc
    11821 gcaactggcg caggcagaag agaaactcgg cgacagcgaa ctgtatgacc agagccgtaa
    11881 agcggagttg accgcctgcc tgcaacagca agccagcgcc aaatccggcc tggaagagtg
    11941 cgaaatggca tggctggaag cccaggagca gcttgagcag atgttgctgg aaggccaaag
    12001 caactgatgg cgcagataac gacgaccgat gccaatgaat tcagcagcag tgctgaattc
    12061 actcctatgc gcggctttag caattgtcat ctgcaaacca tgctgccgcg tctgtttcgt
    12121 cgcaaggtga aattcacccc gtactggcag cggctggagt tgcccgacgg cgattttgtc
    12181 gatctcgcgt ggagtgaagc ccctgcacag gctcggcata aaccgcgttt agtggtattt
    12241 cacgggctgg aaggcagtct caatagccct tacgcccacg gtctggttga ggcggcgcaa
    12301 aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat gcagcggtga accaaaccgt
    12361 atgcaccgca tttaccattc gggcgaaacc gaagacgcca gttggttttt acgctggctg
    12421 caacgcgaat ttggtcatgc gccaacggct gccgtcggct attcgctcgg cggtaatatg
    12481 ctggcctgtt tgctggcaaa ggaaggcaat gatctcccgg ttgatgcggc ggtgattgtc
    12541 tctgcgccgt ttatgctgga agcctgtagc tatcatatgg aaaagggctt ttcccgcgtt
    12601 tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg cgcgcaagct ggcagcctac
    12661 cccggaacgc tgccgattaa tctcgcgcag ttaaaatcgg tacgtcgcat ccgtgaattt
    12721 gacgatctaa tcaccgccag aattcacggc tacgccgacg ctatcgacta ttatcgtcag
    12781 tgtagcgcca tgccgatgct gaaccggatc gccaaaccga cgctgattat tcacgccaaa
    12841 gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg aaagtctccc ctcgcaggtg
    12901 gagtatcaac tgactgaaca tggcggtcat gttggcttta ttggcggtac attacttcat
    12961 ccgcaaatgt ggctggagtc acgcattcct gactggttaa caacgtatct ggaggcgaaa
    13021 tcatgttgat tccgtggcaa gacctctccc ccgaaacgct ggaaaattta attgaaagct
    13081 ttgtgttacg tgaaggcacc gattatggtg aacatgagcg tacacttgaa cagaaagtcg
    13141 ccgacgtcaa acgccagcta cagtgcggag aagcggtgct ggtatggtcg gagctgcacg
    13201 aaacggtcaa tattatgccg cgcagtcagt ttcgcgaata acctgcaacc gtcggcggac
    13261 gagataataa tgatagctaa gtcatggagt tactatgtct gccaaacatc cggtcattgc
    13321 ggtaacagga tccagcggcg cggggaccac caccaccagc ctcgcgtttc gtaaaatatt
    13381 cgcgcagtta aatctgcatg cagctgaggt ggaaggcgac agttttcacc gttacacccg
    13441 cccggaaatg gacatggcga tccgcaaagc gcgcgacgcc gggcggcata tcagctactt
    13501 cggccccgaa gcgaacgact tcggcctgct ggaacaaacc ttcattgaat acggtcagag
    13561 cggcaaaggg aaatctcgca aatatctgca tacctacgac gaagccgtac cctggaatca
    13621 ggtaccggga acattcaccc cctggcaacc tttaccagaa cccactgatg tactgtttta
    13681 tgaaggttta cacggcggcg tagtcacgcc acagcataac gttgcgcagc atgtggactt
    13741 actggtcggc gtagtgccta tcgttaacct tgagtggatt caaaaactga tccgcgacac
    13801 cagcgagcgc gggcactcac gagaagcagt gatggactcg gtagtgcgtt caatggaaga
    13861 ctatatcaac tacatcacac cgcagttttc ccgcacccat cttaacttcc agcgcgttcc
    13921 caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc ccgtcgctgg atgaaagctt
    13981 tgtggtgatc cattttcgta atctggaagg gatcgatttc ccctggctgc tggcgatgtt
    14041 gcaaggctca ttcatttccc acatcaatac gttagtggtg cccggcggca aaatgggtct
    14101 ggcaatggaa ttaattatgc tgccgctggt gcaacgattg atggaaggaa agaaaatcga
    14161 gtaactctgc tattacgccg gataaaatac tatccggctt cacaacggga tagttaaatc
    14221 acgcggcaac cacttcatac gagtgagtaa tattcaccgc tttttccagc atcaacgcca
    14281 ctgaacaata tttctcagca gagagatcaa ccgcacgcgc aaccgctgcg tctttcaggt
    14341 cgcgaccggt gacgataaaa tgcagattaa tgtgcgtaaa caggcgtggt gcctcttcgc
    14401 ggcgttcaga ggtcaatttt acttcacaat cgaccacatc ctgacgccct ttttgcagga
    14461 tcgaaaccac atcgatggca ctgcaaccac ccgccgccat caacaccatt tccatcggac
    14521 ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg atgaccagag gcggattcgc
    14581 ccagaaaagt taacccttcg acccacttca ctcgcgcttg catttttgct actccactgc
    14641 gtcaattttc ctgacagagt acgcgtacta accaaatcgc gcaacggaag gcgacctggg
    14701 tcatgctgaa gcgagacacc aggagacaca aagcgaaagc tatgctaaaa cagtcaggat
    14761 gctacagtaa tacattgatg tactgcatgt atgcaaagga cgtcacatta ccgtgcagta
    14821 cagttgatag ccccttccca ggtagcggga agcatatttc ggcaatccag agacagcggc
    14881 gttatctggc tctggagaaa gcttataaca gaggataacc gcgcatggtg cttggcaaac
    14941 cgcaaacaga cccgactctc gaatggttct tgtctcattg ccacattcat aagtacccat
    15001 ccaagagcac gcttattcac cagggtgaaa aagcggaaac gctgtactac atcgttaaag
    15061 gctctgtggc agtgctgatc aaagacgaag agggtaaaga aatgatcctc tcctatctga
    15121 atcagggtga ttttattggc gaactgggcc tgtttgaaga gggccaggaa cgtagcgcat
    15181 gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc gtacaaaaaa tttcgccaat
    15241 tgattcaggt aaacccggac attctgatgc gtctgtctgc acagatggcg cgtcgtctgc
    15301 aagtcacttc agagaaagtg ggcaacctgg cgttcctcga cgtgacgggc cgcattgcac
    15361 agactctgct gaacctggca aaacaaccag atgctatgac tcacccggac ggtatgcaaa
    15421 tcaaaattac ccgtcaggaa atcggtcaga ttgtcggctg ttctcgtgaa accgtgggac
    15481 gcattctgaa gatgctggaa gatcagaacc tgatctccgc acacggtaaa accatcgtcg
    15541 tttacggcac tcgttaatcc cgtcggagtg gcgcgttact gagtagcgcg ccattttgtt
    15601 tcccccgatg tggcgcagac tgatttatca ccccgatatt aactatgcac tgcggcaaac
    15661 gctggtgctc tgtttgcccg tggccgttgg gttaatgctt ggcgaattac gattcggtct
    15721 gctcttctcc ctcgttcctg cctgttgcaa tattgcgggc cttgatacgc ctcataaacg
    15781 ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgca gcctgtagct tgctgacaca
    15841 gctgctactg gcaaaagatg ttcccctgcc ctttttgctg accggattaa cgctggtact
    15901 tggcgtcact gctgagttgg ggccattgca cgcaaaattg cttcccgcat cgctgctggc
    15961 agccattttt accctcagtc tggcgggata catgccggtc tgggaaccgt tgctcatcta
    16021 tgcgttgggc actctctggt acggattgtt taactggttt tggttctgga tctggcgcga
    16081 acaaccgctg cgcgagtcac taagtctgct gtaccgtgaa ctagcagatt attgtgaagc
    16141 caaatacagc ctgcttaccc agcacaccga ccctgaaaaa gcgctgccgc cgctgctggt
    16201 gcgccagcaa aaagcggtcg atctaattac ccagtgctat cagcaaatgc atatgctttc
    16261 cgcgcaaaat aatactgact acaagcggat gctgcgtatt ttccaggagg cgctggattt
    16321 acaggaacat atttcggtca gtttgcatca gccggaagag gtgcaaaagc tggtcgagcg
    16381 tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc gtcgccgctc gcctgcgcgt
    16441 gctggctgat gacattcttt accatcgcct gccaacgcgt tttacgatgg aaaagcaaat
    16501 tggcgcactg gaaaaaatcg cccgccagca tccggataat ccggttgggc aattctgcta
    16561 ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa aaaccgctct atgcccgtga
    16621 cttactggcc gataaacagc ggcgaatgcc gttacttccg gcgctgaaaa gttatctgtc
    16681 actaaagtct ccggcgctac gcaatgccgg acgactcagt gtgatgttaa gcgttgccag
    16741 cctgatgggc accgcgctgc atctgccgaa gtcgtactgg atcctgatga cggtattgct
    16801 ggtgacacaa aatggctatg gcgcaacccg tctgaggatt gtgaatcgct ccgtgggaac
    16861 cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt aaaattcccg aaggttacac
    16921 cctgacgttg atgctgatta ccaccctcgc cagctacctg atattgcgca aaaactacgg
    16981 ctgggcgacg gtcggtttta ctattaccgc agtgtatacc ctgcaactat tgtggttgaa
    17041 cggcgagcaa tacatccttc cgcgtcttat cgataccatt attggttgtt taattgcttt
    17101 cggcggtact gtctggctgt ggccgcagtg gcagagcggg ttattgcgta aaaacgccca
    17161 tgacgcttta gaagcctatc aggaagcgat tcgcttgatt cttagcgagg atccgcaacc
    17221 tacgccactg gcctggcagc gaatgcgggt aaatcaggca cataacactc tgtataactc
    17281 attgaatcag gcgatgcagg aaccggcgtt taacagccat tatctggcag atatgaaact
    17341 gtgggtaacg cacagccagt ttattgttga gcatattaat gccatgacca cgctggcgcg
    17401 ggaacaccgg gcattgccac ctgaactggc acaagagtat ttacagtctt gtgaaatcgc
    17461 cattcagcgt tgtcagcagc gactggagta tgacgaaccg ggtagttctg gcgatgccaa
    17521 tatcatggat gcgccggaga tgcagccgca cgaaggcgcg gcaggtacgc tggagcagca
    17581 tttacagcgg gttattggtc atctgaacac catgcacacc atttcgtcga tggcatggcg
    17641 tcagcgaccg catcacggga tttggctgag tcgcaagttg cgggattcga aggcgtaatg
    17701 caggctaaac catcggataa ggcattcacg ccgcatccga catcttttgc ctgatgcttc
    17761 gtatttacgc cccaaccacc ttcgccaccg cgtgggcgaa acgttgcatc ccttcatcga
    17821 tatccgcatc ttccaccacc agcgacggtg caaaacgcat cacatccggt ccggcattca
    17881 gcaccattac gccagcctct gcgcccgcat acaggaaatc acgcgcccga cctttgtact
    17941 gtggtttcag ctctgcgcca atcaacagcc ccataccgcg aatatcgcta aacacatcgt
    18001 actgctgatc gatcttctgc agatggtcaa caaaacgctg gcgtttcgcc tgaatgcctt
    18061 ccagcacttc aggggtattg atgatatcaa acgccgcccc cgctactgca caggccagag
    18121 gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcagaagca atttccgccg
    18181 tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag gtcagaatat
    18241 ccggcgtaac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc atcccgcact
    18301 gcacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc aagccctgca
    18361 aaaactctgg cgtcgctgcc gtcacaccgc cctcgccctg gatcggctca accaccaccg
    18421 cacaggtgtg atcatccatc accgctttca ctgcgtggag atcgttaaaa ggaacgtgga
    18481 tgatgtctgc cggttttggc ccaaagccgt cggaatattt tggctgcccg cccaccgaga
    18541 cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg gttttgaacg
    18601 gactatgacg cacacaggcg taatggcgtg ccagtttaaa ggcggtttcg ttagcttccg
    18661 tgccggagtt cataaacacc acgcgttcgg caaacgtcgc ctcaatcagt ttgcgcccaa
    18721 gacgcagcgc cggttcattg gtgaaaacgt tactgatatg ccacagagtt tcgccctggg
    18781 tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact gcaatgccac
    18841 ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc tgacctttta
    18901 ccggaatgaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg
    18961 taattgctgt ttgttcaatt gccatctcat gatcaccctg ttacgcataa ataaatgtga
    19021 aattataatc acaaaatatg cataaaaaat cattgaatgg caatcagaaa tcagcgatgc
    19081 aggaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg atggaactgc
    19141 acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt ttcgctccag
    19201 gccgtcacgt caaagcatgc tggtaatgag ccaggttcca ccaccagcga atggtagcgt
    19261 gtcacggtaa gtggatttgc cagcccccga aatacgccct caccgttatg tgtaatcggc
    19321 gaggttttgc cgtgcatgac ctttgcggcg cgcacaactt tgccgccaaa tgcctgcgcc
    19381 attgcctgat gaccgaggca gacgccaaga atcggcaagc gcccggcata gtggcgaata
    19441 acgtcaaggg agatcccggc ttcatctggc gtacaggggc cgggtgatat gacaattttt
    19501 tgtggtttaa gggcgtcgat atccgccagc gtcaacgcat cgttgcgctt aaccagcaca
    19561 tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc gtagttatct
    19621 ataagcagga tcatggcggc tccggtacga aagaaccgcg ctattttact cagattcccc
    19681 ggcttcgctt accactttgc taaatatcat ctgcagcgcg gtgagatccc ccattgcccc
    19741 actctgattt gcctgattcc aggcctcttt ttcgataccc tgccagctca gttgataccc
    19801 ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc agaccacttc ccacccggaa
    19861 gggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga acttcgcttt
    19921 ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat tgccctcttt
    19981 ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcaa cttcacgcag
    20041 ttgccctgcc cagtcgaaaa tatcctgata cagctggcga tggatagtgc gcaaatgcgg
    20101 taaaccgcgc accagcggac caagctcaat ggtcgcagca cgcagcgccg tcatttcgta
    20161 agcggcctgt tccagccgtt gttgctggcg gatgttcagc cggttacgca tgatatcaag
    20221 gccaggataa agatacggat cgcgcccttc gccgaattta tcgctcatag tgactcctca
    20281 gctcttcaag gcgcgctaaa gcctctgcgg cagtaagagt gactaaaggc atctcgacgc
    20341 cttcaagacg gcgactagcc tgaaaattac gattacgctg aagctcccag agacgggact
    20401 tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat ttgccacaag
    20461 tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc
    20521 gagaaatcat tacggcagga ctttagcgga aaggataact accggttttg acggcacatt
    20581 ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc ttatcggcca cgtccatgcc
    20641 tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccatggt caaggaaggc
    20701 gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag cggtacgcgc
    20761 catcgcgatg gtaccacgcg tgttgcgcag gccgttatcg gcttcatttt tgattggcgg
    20821 gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca taaagccagg
    20881 aatgacgcgg tgaaaggtag tgttgttata aaaaccgctg ttcacataat cgacaaagtt
    20941 ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac cagctgaggt
    21001 tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa gagcagaaag
    21061 agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc cttaacaaac
    21121 agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa ggccatccct
    21181 ttacctaatt ttacgtatct gaaatatatg taacctgacg atcacttttt attccgtgat
    21241 caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt taaacagatc
    21301 acaaaattac ctaaaatcgc ccgctcgcgg catatcccgc tatgccattt tatttttgaa
    21361 cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag ctttctctcc
    21421 tacgcactga ctggtgcgtt ggttattgtc accgggatgg tgatgggaaa tatcgccgat
    21481 tatttcaatc tgcctgtttc cagtatgagt aacaccttca ccttcctcaa cgccggcatt
    21541 ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa aacgcagtta
    21601 cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag ccacagcctg
    21661 gcgctgttct cggcggcgat gttcattctc ggggtggtca gcggcatcac catgtcgatt
    21721 ggtacattcc tggtaacaca aatgtatgaa gggcgtcagc gcggttcccg cctgttattt
    21781 accgactcct tcttcagtat ggctgggatg attttcccaa tgatcgccgc gtttctactg
    21841 gcgcgcagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta tgtcgctatt
    21901 tttattctga ccttcggctg tgagttcccg gcgctgggca aacatgcgcc aaaaacggat
    21961 gctccggtag agaaagaaaa gtgggggatc ggcgtactgt ttctctctgt tgcggcgctg
    22021 tgctacatcc tcggtcagtt aggttttatc tcctgggtgc ctgagtatgc caaaggcctg
    22081 ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc atacatggtc
    22141 ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat tctgaccgta
    22201 ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc agcacatatg
    22261 gcgtggtcaa ttctcgctct gggcttcttc tccagcgcga tctataccac catcatcacc
    22321 ctgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc
    22381 ggaaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga acatagcggt
    22441 ccgcaggcgg cactgcttac agcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc
    22501 ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca ttaattgcta
    22561 atgccggacg gcactatcgt cgtccggcct tttcctctct tcccccgcta cgtgcatcta
    22621 tttctataaa cccgttcatt ttgtctattt tttgcacaaa catgaaatat cagacaattc
    22681 cgtgacttaa gaaaatttat acaaatcagc aatatacccc ttaaggagta tataaaggtg
    22741 aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa
    22801 gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg tatggtcggc
    22861 catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga tattaccgtt
    22921 ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta cttctctcac
    22981 cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa
    23041 gttctggtcg gcgaacgcgc tatcaccatc aaccgtcagg agaaggtgat tcactccagc
    23101 gccggacgta ccgtttttta tgacaagctg atcatggcaa ccggttccta cccgtggatc
    23161 ccaccaatca aaggttctga tactcaggac tgctttgtct atcgcaccat tgaagacctc
    23221 aacgccattg aatcctgcgc ccgtcgcagc aaacgcggtg ccgttgttgg tggcggcctg
    23281 ttaggtctgg aagccgctgg cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa
    23341 tttgccccta tgctgatggc ggaacagctt gatcagatgg gtggcgagca gctgcgtcgc
    23401 aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acacccttga gattgtgcag
    23461 gaaggtgtcg aagcgcgtaa aaccatgcgt tttgccgacg gcagcgaact ggaagtcgac
    23521 tttatcgtct tctctaccgg tatccgtccg cgcgataagc tggcaaccca gtgtggtctg
    23581 gacgttgctc cgcgtggggg tattgtcatt aatgattcct gccagacttc cgatccggat
    23641 atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct ggtagcacca
    23701 ggctacaaaa tggcgcaggt cgccgttgac catattctcg gtagcgaaaa cgcctttgaa
    23761 ggtgctgacc ttagcgccaa gctgaaactg ctgggcgtag acgtaggcgg tattggtgat
    23821 gcgcacggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag caaagagatc
    23881 tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt actggtgggc
    23941 gataccagcg actacggcaa cctgctgcaa ctggtgctga atgctatcga gctgccggaa
    24001 aacccggatt ctctgattct gccggcgcac tcgggtagcg gtaagccgtc tatcggtgtt
    24061 gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcaccaa aggtgatctg
    24121 attgctgcca tcaacaaagg ctgccacaca gttgcggcgc tgaaagctga aaccaaagcg
    24181 ggtactggct gcggtggctg tatcccgctg gtcactcagg tactgaacgc ggaactggcg
    24241 aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc gcgtcaggaa
    24301 ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac
    24361 ggcaaaggct acggttgtga agtttgtaaa ccaaccgtcg gttcgctgct ggcctcctgc
    24421 tggaacgaat acattctgaa gccggaacat actccgctgc aggattctaa cgacaacttc
    24481 ctcgctaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc gggcggtgaa
    24541 atcaccccgg aagggctgat ggcagtgggt cgtatcgcgc gtgaatttaa tctctacacc
    24601 aaaatcaccg gctcccagcg cctggcgatg tttggcgcac agaaagacga tctgccggaa
    24661 atctggcgtc agctgattga agctggcttc gaaaccggtc atgcctatgc gaaagcactg
    24721 cgtatggcga aaacctgcgt gggtagcacc tggtgccgct acggtgttgg cgatagcgtc
    24781 ggcctcggcg tggaactgga aaaccgctac aaaggcatcc gtacgccgca caaaatgaag
    24841 ttcggtgtct ccggctgtac ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatt
    24901 atcgccactg aaaaaggctg gaacctgtat gtttgcggta acggcggcat gaaaccgcgt
    24961 catgcggatc tgctggcagc ggatatcgat cgcgaaacgc tgatcaaata tctcgaccgc
    25021 ttcatgatgt tctacatccg taccgccgac aaactgacgc gtaccgcgcc gtggttagaa
    25081 aacctcgaag gcggcatcga ttacctgaaa gcggtgatca ttgacgacaa actggggctg
    25141 aacgctcatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg tgagtggact
    25201 gaaacggtca atacaccgtc cgcgcagact cgcttcaaac acttcatcaa cagcgacaag
    25261 cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc aacgccgtat
    25321 gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa gacatctgca
    25381 aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggcgac gagcaggtcg
    25441 cgattttccg cccgtatcac agcgaccagg tgtttgcgat cagcaacatc gatccgttct
    25501 tcgagtccag cgtgctgtca cgcgggctga ttgcggaaca ccagggcgag ctgtgggtcg
    25561 ccagcccgct gaaaaaacag cgttttcgct taagtgatgg cttgtgcatg gaagacgaac
    25621 agttttccgt caaacattac gaagcacgag tgaaagacgg cgtggtgcag ctgcgcggtt
    25681 aatgttttaa cgggaggcgc aatgcctccc ctttttgcat ggtcctgtaa tgaccttcgg
    25741 tatattgcag gacatttttt aaactttttg ttttattttt tttgttttta ttttttaaag
    25801 gataatcaaa tgtttacaga cactattaat aagtgtgcgg ctaacgctgc gcgcattgca
    25861 cgcctgtcgg caaataaccc gctcggcttt tgggtcagct ccgccatggc gggcgcgtat
    25921 gtgggtcttg ggatcatctt gattttcacg ctcggtaatt tgctcgaccc gtccgtacgt
    25981 ccgctggtga tgggcgcgac ctttggtatc gccttaacgc tggtgattat cgccggttct
    26041 gaactgttca ccggacacac catgttcctc acctttgggg taaaagcggg cagcatcagc
    26101 cacgggcaaa tgtgggcaat cctgccgcaa acctggctgg gtaacctggt cggttccgtc
    26161 ttcgttgcca tgctctatag ctggggcggc ggtagcctgc tgccggtaga taccagcatc
    26221 gttcactccg tcgcgctggc taaaaccact gcaccggcaa tggtactgtt cttcaaaggt
    26281 gcattgtgta actggctggt ttgcctggca atctggatgg cgctgcgcac tgaaggggcg
    26341 gcgaaattta tcgctatctg gtggtgtctg ctggcattta tcgcgtccgg ctacgagcac
    26401 tctatcgcta acatgacgct gttcgcgctc tcctggttcg gcaaccacag cgaagcctac
    26461 acgctggcgg gtattggtca taacctgctg tgggtgacgc tgggtaatac tttatcaggt
    26521 gccgtattca tgggattggg ttattggtat gctacgccga aagcgaatcg tccggttgcg
    26581 gacaaattta atcaaactga aacggctgcc ggttaattac taaggggttt ttacgtggat
    26641 catttgccta tattttgcca attacgcgat cgcgactgtc tgattgtcgg cggtggtgat
    26701 gtcgcggaac gcaaagcaag gttgctgtta gacgcaggcg ctcgcttaac ggtgaatgca
    26761 ttagcgttta ttccacagtt caccgcatgg gcagatgcag gcatgttaac cctcgtcgaa
    26821 gggccatttg atgaaagcct tctcgacacc tgctggctgg ctattgcagc gacggatgat
    26881 gacgcgctta accagcgcgt cagcgaagcc gctgaagctc gtcgcatctt ctgtaacgtg
    26941 gtcgatgcgc cgaaagccgc cagctttatt atgccgtcga ttattgaccg ctcaccgctc
    27001 atggtagcgg tctcctctgg cggcacctct ccggttctgg cacgcctgtt gcgcgaaaaa
    27061 cttgaatcac tgctgccgtt acatctgggc caggtagcga aatacgccgg gcaattacgc
    27121 gggcgagtga aacaacagtt cgccacgatg ggtgagcgtc gccgtttctg ggagaaattg
    27181 ttcgttaacg accgcctggc gcagtcgctg gcaaacaacg atcagaaagc cattactgaa
    27241 acgaccgaac agttaatcaa cgaaccgctc gaccatcgcg gtgaagtggt gctggttggt
    27301 gcaggtccgg gcgatgccgg gctgctgaca ctgaaaggac tgcaacaaat tcagcaggca
    27361 gatgtggtgg tctacgaccg tctggtttct gacgatatta tgaatctggt acgccgcgat
    27421 gcggaccgtg ttttcgtcgg caaacgcgcg ggataccact gcgtacccca ggaagagatt
    27481 aaccagatcc tgctgcggga agcgcaaaaa ggcaaacgcg tggtgcggct gaaaggtggc
    27541 gatccgttta tttttggccg tggtggcgaa gagctggaaa cactgtgcaa cgcgggtatt
    27601 ccgttctcgg tggttccggg tattaccgca gcttctggtt gctctgccta ttcgggtatt
    27661 ccactcacgc atcgcgatta tgcccagagc gtacgcttaa ttaccggaca cttaaaaacc
    27721 ggtggcgagc tggactggga aaacctggcg gcagaaaaac agacgctggt gttctatatg
    27781 gggttgaatc aggccgcgac tattcagcaa aagctgattg aacacggtat gcctggcgaa
    27841 atgccggtgg caattgtcga aaacggaacg gcagtcacgc agcgcgtgat tgacggtacg
    27901 ctcacgcaac tcggtgaact tgctcagcaa atgaacagtc catcgctaat tattattggt
    27961 cgggttgttg gcctgcgcga taaactgaac tggttctcca accattaatt taacccgggc
    28021 cagagaattc tggccttctt aatgactctt tttatatatt cagcaaataa aacatagccc
    28081 cttaataaat attatctgct aatgggttat attcaccgtt acagcgactt acaaaattaa
    28141 gtcattcgct cactgacgcc aattaaatat tcctgtatcg ttcttaacga gcagtctgct
    28201 tatattcata taatcaa
//
LOCUS       DAPHJE010000057        28160 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPHJE010000057 DAPHJE010000000
VERSION     DAPHJE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.057"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..634
                     /locus_tag="RH222_002463"
     CDS             <1..634
                     /locus_tag="RH222_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3797465.1"
                     /translation="ALGLVTTAGLAWVAKYLEITATKYDSPQMYVAIGLLLLCMYGLS
                     KDINKINAVIAGVMYLFLLSLVAIVVASLAPVPAIIVVFSTAGAMFLISMLAGLLFNV
                     DPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISCLMIVLWSGIISHGRNKLLELA
                     GKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(670..1626)
                     /locus_tag="RH222_002464"
     CDS             complement(670..1626)
                     /locus_tag="RH222_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV3797466.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1626..2867)
                     /gene="clsB"
                     /locus_tag="RH222_002465"
     CDS             complement(1626..2867)
                     /gene="clsB"
                     /locus_tag="RH222_002465"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV3797467.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2864..3625)
                     /locus_tag="RH222_002466"
     CDS             complement(2864..3625)
                     /locus_tag="RH222_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV3797468.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3758..4168
                     /locus_tag="RH222_002467"
     CDS             3758..4168
                     /locus_tag="RH222_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV3797469.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4130..5236)
                     /locus_tag="RH222_002468"
     CDS             complement(4130..5236)
                     /locus_tag="RH222_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3797470.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5247..6380)
                     /locus_tag="RH222_002469"
     CDS             complement(5247..6380)
                     /locus_tag="RH222_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3797471.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6373..8109)
                     /locus_tag="RH222_002470"
     CDS             complement(6373..8109)
                     /locus_tag="RH222_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3797472.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVDGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8102..9097)
                     /gene="hlyD"
                     /locus_tag="RH222_002471"
     CDS             complement(8102..9097)
                     /gene="hlyD"
                     /locus_tag="RH222_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV3797473.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLILA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9100..9771)
                     /gene="cecR"
                     /locus_tag="RH222_002472"
     CDS             complement(9100..9771)
                     /gene="cecR"
                     /locus_tag="RH222_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV3797474.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10000..11364
                     /gene="rhlE"
                     /locus_tag="RH222_002473"
     CDS             10000..11364
                     /gene="rhlE"
                     /locus_tag="RH222_002473"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV3797475.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11596..12078)
                     /gene="ybiA"
                     /locus_tag="RH222_002474"
     CDS             complement(11596..12078)
                     /gene="ybiA"
                     /locus_tag="RH222_002474"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDV3797476.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12198..14348
                     /gene="dinG"
                     /locus_tag="RH222_002475"
     CDS             12198..14348
                     /gene="dinG"
                     /locus_tag="RH222_002475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV3797477.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVTKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14376..15338
                     /gene="ybiB"
                     /locus_tag="RH222_002476"
     CDS             14376..15338
                     /gene="ybiB"
                     /locus_tag="RH222_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV3797478.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15479..16564
                     /gene="hcxB"
                     /locus_tag="RH222_002477"
     CDS             15479..16564
                     /gene="hcxB"
                     /locus_tag="RH222_002477"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV3797479.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16793..17053)
                     /locus_tag="RH222_002478"
     CDS             complement(16793..17053)
                     /locus_tag="RH222_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3797480.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17318..17584)
                     /gene="ybiI"
                     /locus_tag="RH222_002479"
     CDS             complement(17318..17584)
                     /gene="ybiI"
                     /locus_tag="RH222_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV3797481.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17658..18335)
                     /gene="ybiX"
                     /locus_tag="RH222_002480"
     CDS             complement(17658..18335)
                     /gene="ybiX"
                     /locus_tag="RH222_002480"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV3797482.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18377..20659)
                     /locus_tag="RH222_002481"
     CDS             complement(18377..20659)
                     /locus_tag="RH222_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDV3797483.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20924..21184)
                     /locus_tag="RH222_002482"
     CDS             complement(20924..21184)
                     /locus_tag="RH222_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3797484.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21460..22386
                     /gene="rlmF"
                     /locus_tag="RH222_002483"
     CDS             21460..22386
                     /gene="rlmF"
                     /locus_tag="RH222_002483"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV3797485.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22383..24608)
                     /gene="ybiO"
                     /locus_tag="RH222_002484"
     CDS             complement(22383..24608)
                     /gene="ybiO"
                     /locus_tag="RH222_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV3797486.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(24869..25591)
                     /gene="glnQ"
                     /locus_tag="RH222_002485"
     CDS             complement(24869..25591)
                     /gene="glnQ"
                     /locus_tag="RH222_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV3797487.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25588..26247)
                     /gene="glnP"
                     /locus_tag="RH222_002486"
     CDS             complement(25588..26247)
                     /gene="glnP"
                     /locus_tag="RH222_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV3797488.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26386..27132)
                     /gene="glnH"
                     /locus_tag="RH222_002487"
     CDS             complement(26386..27132)
                     /gene="glnH"
                     /locus_tag="RH222_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV3797489.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27536..28039)
                     /gene="dps"
                     /locus_tag="RH222_002488"
                     /gene_synonym="pexB"
     CDS             complement(27536..28039)
                     /gene="dps"
                     /locus_tag="RH222_002488"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV3797490.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         6659 a         7326 c         7501 g         6674 t
ORIGIN      
        1 cgctttaggt ttggtgacaa ccgcaggact ggcctgggta gcgaagtatc tcgaaattac
       61 ggcaacaaaa tatgattcac cgcaaatgta tgtcgccata ggattattat tactttgtat
      121 gtatggctta agtaaggata tcaacaagat aaatgccgtc atcgcaggcg taatgtatct
      181 atttttactc tctttggtgg cgattgtcgt tgctagttta gctcctgtac ctgccattat
      241 cgtcgtgttc agcactgcgg gcgcgatgtt tttaatcagt atgctggccg gtttattatt
      301 taatgttgat cctggttctc accgttttat cattatgatg acgttgacag ggttggccct
      361 ggtgatcatc atgaatgccg cattaatgag tgaacgaccc gtttgggtaa taagttgctt
      421 aatgattgtg ttatggtcag gcattatctc gcatggacga aataagctcc ttgaattggc
      481 ggggaaatgc catagtgaag agttgtggag tccggttcgt tgcgctgtta caggtgcatt
      541 aacactctat tactatttta tcggcttctt tgggatactt gccgcgatag ctataacgct
      601 tgtctggcaa aggcatacgc gtttttttca ttagtcgtaa atgcagatac taaagaactt
      661 aactaaactt cacatcgccg cttcattttt cgcccgtagc ttcttcgcct ggctttccag
      721 tagcaggtaa cagatcagcg ccagcagcag cgggataaag taatacagga cgcgataagc
      781 gagtagggcg gcaataattg tgcctttgga tgtatgctcc ccagccagta gcgcgataaa
      841 caccgcttcc agcacaccaa ttcccgccgg aatatgcacg atgacaccag caatactact
      901 aaccagcaac acgcccagta caaagaaata gttcacgctt tgaccaagta acagccagat
      961 aatcgccccc attaccatcc agttaacgct ggaaatcagc atctgagcga gggcgaattt
     1021 ccatgaaggc agcaccagct tttgcccttt gatggtcata tggcggtgct tcgcgaaagc
     1081 gcaaaaccac aaataaaccg cgataatcat cagtaagcca atgccgagaa tacgcagcgt
     1141 agtttgatcg acataccagt gatccggcaa ctccaccacg cctgcggtaa agataatccc
     1201 tgccagtaaa atatagccca gccagttggt ggtaatactg agcgagaaaa tccgcgtaat
     1261 agtgctgccc ggtaacccta gtcgggagta caaacgatag cgcataccaa tgccgccgac
     1321 ccaggtactg agcgtcaggt tgaaggcgta gcagataaac gacaccagca tcacctggcg
     1381 cttcgccagt ttgtgaccgc agtaaaagcg ggcgagcagg tcatagcagc cgtaaatcag
     1441 atagctgacg accaccagcc cgaccgcact aagcagcgca acgcgattgt agtcgcggat
     1501 gaccttccag acctcttccc agtccacttt tttggcgtag accaccagca acacaatcac
     1561 cgcgataaaa aacagccagg tgaggatctt ctttgctaag cgccaacgcg ggtgtgattt
     1621 actcatcagg gttttacccc cgtgttttca gtttctaccc gatcctgcgt ttccattgtc
     1681 ggttgtgcgg gcggatcaac ctgcgccaga cgtggtgtgt gtgccggaag ccagccaacc
     1741 agcgccggga agtggcgtaa aaagtggaac gccagcacgc ttttggtcag gttccaccag
     1801 gtgcgtttcg gcagcatggt ctcatcgacc tgctgacaat ctgcggcaat aatgccgttc
     1861 agattatcgc gcagcgtctg gttaaaatga cgatcgtgga tgatgacatt tgcttcgaga
     1921 ttcagtgaca aactgagcgg atcgagatta ctggacccta ctgtcgccca gtgatcgtcc
     1981 atcaatgcca ctttgccgtg gagcgggcgg cggcggtact caaatacctg aacgccgcct
     2041 ttaaccagat agttatacag caagcgcgca ccgactctga caatcggcat atccggttcg
     2101 ccctgaatga tcagtttgat ccgcaccccg cgccgtgccg ctttacgcaa ggcgtgtaaa
     2161 aatcgatagc cggggaagaa gtaggcgttg gcgataatga cttctcgccg cgcctgagtg
     2221 agcattttca gatagtgacg ttcaatatca tcgcgatgtt cttcgttatc gcgccagacc
     2281 agcaatacct gcgcttctcc cggctggcgg ttctcttccg ctttgtgatg acgtcgccac
     2341 cagcgtcgtg ccgcgctctg tccaggcagg ttttccagct caaactggag aatatcttcg
     2401 acaatcggcc cttcaaggcg taccgcgtaa tcctgtttag cctctggacc gtagctggac
     2461 atatgctcgg cggagtaatt cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca
     2521 attttgcgat gcatccggcg aaacacattg gtgcgcatac caaaaaggcg agggcgggga
     2581 tcgtagtagc gaaataccac gccagctgcc gtcagttcat tgacaaactc atcgctgaga
     2641 tccggcgaac cgtagccatc cagcaagact tccgctttaa ccccgcgttg cgctgctgcc
     2701 agtagtgccg catgcagctg tttgccgacg tcatcctcaa accagataaa cgtttcaaga
     2761 atgatgcgtt cttgtgcctc gccaatcgcc ttaaacaccg cgggataata ttgctcgccg
     2821 ttttccagca actggatctt attgccttcg cgccagctac atttcataaa tgaatctccg
     2881 cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg
     2941 cgctggcgct ggcatttttg acgtagatcc tgtccagtcg tagtagagga aattgcaccg
     3001 gaaacgtgcg cgccgggcgt ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca
     3061 cttttaacgg atgattagct ttttgccgcc agtcattgaa atcacccgcc accaataccg
     3121 gttcgccgtc cggtagctca ttcacccatt cggcgagcat cgcaagctgc gcctgacggt
     3181 gcgcctcacg caggcccaga tgtacgcaca tcacatgaat cgcttttccg gtcatcggtg
     3241 gcacgatgcg gcagtagagc acgccgcgct tttccgcacc atcgaccgaa acatcgcgat
     3301 tctcataatg ttcaatggga taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt
     3361 atacggcatt gcgaccgtag gcaaaatcgc tccacatcgt gtcggcgaga aactcgtagt
     3421 gcgaggtatc gggccagttt tccacatgca gcggatgaac ttcgtgcgcg cccatcactt
     3481 cctgcaggca aacaatatcg gcgctgacgg tacgcaccgc gtcgcgaagt tccggcaaaa
     3541 tgaagcgtcg gttaaacgcg gtaaaacctt tgtgaatgtt gatggtgagc accttgaacg
     3601 aaaattgttg tgtttgatcg ggcataattt tcctgtcttt gcctcttatc tcattgaaat
     3661 agtgtagtcg gcgtcacaaa aaggtgcggt cttacggaat tttccgtaaa gttcggtact
     3721 ctgagtaagt agagataaat tcttcaggag agaagccatg aagtggcaac aacgtgttcg
     3781 tgtcgcaact ggtctaagtt gctggcagat tatgttgcat ttactggtag tggcgctgct
     3841 ggtggtgggc tggatgagta agactctggt tcacgtcggc gtgggattat gcgcactgta
     3901 ttgtgtcacg gtagtgatga tgttggtgtt tcagcgccac cccgagcaac gctggcgtga
     3961 ggtggcagac gtgctggaag agctgaccac gacctggtat tttggcgcag cgctgattgt
     4021 gctgtggctg ttgtcccgtg tactggaaaa caactttttg ctggcaattg cagggctggc
     4081 aatccttgcc ggcccggcgg tagtgtcttt gctggcgaaa gataagaagt tacatcacct
     4141 tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc
     4201 acaaactatt ccacacaata tccagactcg catccttcaa ataaatctgc ttggtaatgt
     4261 ccgtaaagtg gcgaataggg ttaatccacg tcaggttttg cagccatact ggcatgtttt
     4321 ccaccggaga aacgtaaccg gaaaggagaa tggcgggcat cataaaaaca aacacgccga
     4381 taaacgcctg ctgttgtgtc gagcagagtg atgaaatcaa cagaccgaat cccaccagcg
     4441 ataaaccgta aatcaccatc gtaaagtaga acagcgccag cgatccggcg aaggggattt
     4501 gatacgccca gataccaatc gccagcacaa tggtggcctg gaaggtggcg acaattaacg
     4561 ccggtacggc tttgccgatg aagatctgcc aggtggtgag cggcgaaacc agtagctgat
     4621 cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga agtgacgatc attacgccga
     4681 tagtggtgat catggcgatc agcgacggca ccacaaacca tttgtagtca agattcgggt
     4741 tataccagtt gcgtaccacc agctcgctgt tgttaggttt cggttttcct tccagcagct
     4801 cctgctgata atttttgacg atctgttgca ggtagttggc ggcaatttgc gcactgttgg
     4861 agttacgccc gtcgaggatc aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg
     4921 agaagtcagc cgggaaacgc accagtagta acgccttttg tgtgtcgatg gttgggcgga
     4981 tctcctgtgg gcttttcagc agcagcacat gagtaaaggc gctggcgcgg gcaaaacgtt
     5041 gggtcagctc caccgaatgc tcgccgttat cttcatcgta gatggcgatg gtggcgttag
     5101 tcacttccag cgtggcggcg aacgggaaca gaatcacctg aataagcaca ggtaaaatca
     5161 gaatcgcgcg ggtttgcggt tcgcgcagca gcgactgcaa ctctttgcgg attaacgtcc
     5221 ataagcgatg aaacatgctc ttctccctaa tccagccgac gtttggtttt cagccacgtc
     5281 aggccgataa acatcaccgc cgaagcgatc aaaaacagca cgtttaccac cagcaccact
     5341 ggaatattcc cggcgaggaa caggctttgc agggtgctga cgaaataacg agcgggaata
     5401 atgtacgtca ccgcgcggat caccgcgggc atactgtcga tctgaaaaat aaagccggaa
     5461 agcataatcg acggcagaaa agcggcgttc agggcgacct gagcggcatt gaactggttg
     5521 cgggtaatcg tggaaatcag cagccccatc cccagggtac tgagtaaaaa caggctggag
     5581 ataaaaaaca gaatcagcag cgacccgcga tacggcacgc cgagaataaa cactgacacc
     5641 agcatacaca gcaacatcgc cagcatcccg agaaagtaat aagggatcag cttacacagc
     5701 agcagttccg tgcgggtaat ctccgtagag agcagagcct ccatggtgcc gcgttcccat
     5761 tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga ccgtcatgat aatggtcacc
     5821 gcaccgggga taatgaagtg ctggctaatc gccgccgggt taaaccagta gcgggtttgt
     5881 acatcaataa gcggttcaaa agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc
     5941 tgccagatcc cttcgacata cccctgtaca aagttagcgg tattcggctc actgccgtcg
     6001 gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc
     6061 ggaataacca ccagaccgcg aattttcccc gcctgcattt tggcgatcag ttcctgacgg
     6121 ttatcgctga tggtggcgtc gatgtagggc gaaccggtca tggtgtgggt gaaatccagc
     6181 gccgcttcgc tacgctgttc cagtaaaatc ccgacccgca gcttgctgga gtcgaggtta
     6241 atgccgtaac caaaaataaa cagcagtagc agcgggatca ctaccgcaat cagccagcta
     6301 ctcggatcgc gaacgatctg ccgcgtctct ttaacgcaca gcgcccgtac gcgacgccag
     6361 gacaggatcg ggttactcat tgctatgctc cttatcccag tcgtggatca actgaataaa
     6421 ggcttgctcc atggtgggat cgggttgctc gtcgttggcc gactgcgctt tcaaatcatc
     6481 cggcgtgccg ctggcaatta atttcccacg gtacaccagg ccgatgcggt cgcaatattc
     6541 cgcttcatcc ataaagtggg tggtgaccat caccgtgacg cctttctcta ccatgctgtt
     6601 gatgtgcagc caaaattcac ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag
     6661 aaacagaatg tccggttcat gcatcagcga acaggccagc gccagccgct gtttaaaacc
     6721 taatggcagt tcatcggtgg cgtgggaggc gatacttttc aggccgaatg cctcgctcat
     6781 gcgggagatt ttttcgttct gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg
     6841 taaattctgt tcgaccgtca ggttgccgta gagcgaaaat ttttgcgcca tatagccgag
     6901 atgctggcgt gctttaccgg aactctcttt cagatccatc cccagcacca gcgcctgacc
     6961 agaggtcggc accagcaaac cgcacatcat cttaaaggtg gtcgatttac ccgcgccgtt
     7021 tggccccagc aaaccaaaaa tctccccacg tttaacggca aagttgacgt gatcggtggc
     7081 ggcaaaatca ccaaatttct tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt
     7141 gccgtctacc gtatgcaata ttgcacccag cggcgattct gaggttccgg caccgcccag
     7201 caaatcaata aacgcatctt caaagcgcgg tgtagtttcg ttgatattga tttccggcat
     7261 cccgtcggca tggcgaatat cgtctggtgt ggcctctttt ttgaggatca gacgtaccga
     7321 tttcccctga atcatgccgt cgctgacctg tggcagtttc aaggcgcgtt gcaatagttt
     7381 gcggttgccc tcgtgtggac tggtcatcag aaagctgcgt ccggccatgg tttgtgtcag
     7441 ggcttttggt tccccctgat acagcaactc gccttcgttc atcagcagca cgtcacggca
     7501 ctgctcggct tcgtcgagat acgaggtact ccagaggatt aacatccctt cgcctgccag
     7561 ctcatgcacc atctgccaca gttcgcgccg tgagataggg tcaacgccga cgccgggttc
     7621 atcgagcagc aacactttcg gttcgcccac cagggtacag gccagaccga gtttttgttt
     7681 catcccaccg gagagcttgc ccgccaggcg tccggtaaac ggcccaagag acgtaaactc
     7741 cagcaggcga gcaaaagttt gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta
     7801 cagattgagg ttctccatca ccgtgagatc ttcatacaga ccaaatttct gcggcatata
     7861 gccgagcacg gcgtgcagcg cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc
     7921 attgccgctg tcgggtttca gtaatcccgc cagcatccgc attagcgtgg ttttacctgc
     7981 accatccggc cccaccagcc ccgtcacata accggcgtga atggtacaat cgagcggcgc
     8041 gacggcgggc ttgtccatgc ccggaaagcg tttttccagg ccgttcagcg tgataacggc
     8101 atcattcatg tcctgcctcg ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg
     8161 catcatcggc gtcggtcacc acaatacgca ggcgatagac gaggtcggta cgcagatccg
     8221 gtgtttcgac ggttttcggg gtaaattcag cagtcggcga aacgaaacca atctgcccgt
     8281 gatacggctt gtccgggcga ccatcggtat aaagcagcac tttgcgcccc ggctgggcct
     8341 ggtcaagatt ccgttcatca acataagcgc gcacccacac cggacgcgtt agtgaaacgg
     8401 taaacaccgt gccaccttca ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg
     8461 tgccatcaga cggggctatc aacgttgagt cctgcaaatt caactccgcc tgcgccagtt
     8521 gcgcctgcgc ctgttcgagg ctggctttcg cctgggcgat gtcctgttca cggttaccgg
     8581 aacggtactg acgcaattta tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg
     8641 aggagcgggc attttccagg tcatttgccg aaatggtgcg gcttttccac aacccttgct
     8701 ggcggttgta gaagttctgc gcatagtcat aggcggcctg cgcctgtttt acggctgcgg
     8761 cggcctgagc gatttcttca tcgcgatacc cggcaagtat caggtcatac tgcgcctgcg
     8821 ccaccgaaac acccgctttc gcctgcatca gggcaatctc atacggcttg tgatccagtt
     8881 cgcccagcac ctggcccgct ttgatagcat caccttcgtc caccgccagc gattcaacgc
     8941 gccccccaac acggaaacta agattcaccg tacgaatatc cacgttgcca tacagcgtca
     9001 ggccgttatc ctggcggctt tgataccacc agtagcctcc ggcaaccacg gcggcaagta
     9061 ccactaccgc cagtccgatc acgacaggtt ttttcatcac tacaaactcc tttgcgataa
     9121 tccttgcaaa atcaggtcga tatgacaggt caccgtctgg ttgatcagtt cggtcttttc
     9181 ttcatcgaac gcggtccagc cggtacgtaa cagaattgtt tctttgccaa gacggaacgc
     9241 cagaatctca ccaatcaacg catgggtatg aaggatcatg cgggtgtcat tggcgtcgct
     9301 gccggtccag gcggcaatca ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc
     9361 gtgcaccagg tggtaggctg ccgtgggaga gagctgctca cgggagataa acttgctgag
     9421 gttgacggtg tcatcctggg tgagcagctt aatcatgttc ctgcaggcgc gaaggatcag
     9481 ttcacggatg gcagcccgat caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc
     9541 atgcggacgg aactgctcgc caataaaatc ggcaatccac tgggcgcagg cgaggtacaa
     9601 atcttctttc gaaccgaagt agtaggtgat ggcagcgata ttctgcccgg cctgggcggc
     9661 tatctcgcgg gtggtggcgt tcattccata ttcaccaaac tgcgccagtg cggcagcaat
     9721 cagctgtttt ttcgcctgtt cacccttgat tgtcatggca ggattattca tcgcacagtc
     9781 cattcttaat caaatgattg attaagatta tgactccata tgggagttgt ccagtatggc
     9841 taagaatttt agctacgcca gtcacaggga taatttatgc gctgcgtcac aaaaactgct
     9901 acactccgct ccctcatgac attgtggttt ttgtcatttt ccttttcagt atctccctga
     9961 aaactacacc ggtaacggtc ggggcggttc ggagtagtta tgtctttcga ttctttgggt
    10021 ttaagccctg atatcctgcg cgccgttgcc gagcagggtt accgtgaacc cacccctatt
    10081 cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc tgatggctag cgcccagacc
    10141 ggtaccggta aaacagcggg ctttacgctg ccgctgttgc aacacctgat cactcgccag
    10201 ccgcacgcca aagggcgtcg tccggtacgt gcgctcattc ttaccccgac ccgtgaactg
    10261 gctgcgcaga ttggcgaaaa cgtccgtgat tacagcaaat atttgaacat tcgttcgctg
    10321 gtggtgtttg gcggtgtcag cattaacccg caaatgatga agctgcgcgg cggcgttgat
    10381 gttctggtgg caacaccggg acgtttgctg gacctggaac atcaaaatgc agtgaagctg
    10441 gatcaggttg aaatcctcgt cctcgatgaa gctgaccgca tgctcgacat gggctttatc
    10501 cacgatatcc gtcgcgtgtt aacaaaactg ccagcgaaac gccagaacct gctgttctct
    10561 gcgaccttct ctgacgatat taaagccctg gcggaaaaac tgttgcacaa cccgctggaa
    10621 atcgaagtgg cacgccgcaa taccgcgtct gatcaggtga cccagcacgt tcactttgtc
    10681 gataagaaac gcaaacgcga attgctgtcg cacatgattg gtaaagggaa ctggcagcag
    10741 gtgctggtgt ttacccgtac caaacacggc gctaaccatc tggctgagca gctcaataaa
    10801 gatggcatcc gtagtgcggc gatccacggg aataaatcgc aaggtgcgcg tacccgtgcg
    10861 ctggctgatt ttaaatcggg tgacattcgc gtactggtgg cgaccgatat cgctgcgcgc
    10921 ggtctggata tcgaagagtt gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa
    10981 gattatgtcc accgtatcgg gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg
    11041 ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg aaaaactgct gaaaaaagag
    11101 atcccgcgca ttgcgattcc gggctatgag ccggacccgt caatcaaagc cgaaccgatc
    11161 cagaacggtc gccagcaacg tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa
    11221 cagcaaccac gccgtgggga aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa
    11281 ccgtctcgcc gcctcggcga tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg
    11341 cgtaaacctg ccgctgcgca gtaatctttt atgccgggct atgcccggca tcaggctgat
    11401 gaacaaacgc aaaactgtct gatgcgctac gcttatcagg cctacgtgaa ctctgcaata
    11461 tattgaattt gcatgctttt gtaggccgga taaggcgttt acgccgcatc cggcatttca
    11521 caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca
    11581 taagccaccg agacgttact tctctatagc caattgttcg cgcaactcca ttaaaaggta
    11641 gcccagtcga ttcttgccct taccattacc accgtctccc cagtaagcat cgttttccgt
    11701 atgctcaacc agttttgcgg gcgcggttgc cagcaagagc gcacgcagtt ctgcatgctg
    11761 ttcgaattta gcgcgaagtg cttttcgcat cacctgttct ttgaccgact cccagttttt
    11821 acgcagaggc ttagaacgat cgcgccccat gcgtgcggcg accatagggg aagaaacccg
    11881 acggatctct tcgcggtatt tttcatcaag gaatttttgt gcctgaaaat agtgttctga
    11941 ggtgggccag gttttcccgt caaccttgat gggccaggcg gcaaagttag aaaaatcacc
    12001 gtagtcatcg ctggtgctgt agaaatttat gatggtgtct tgcatgacgt gttggatcct
    12061 ttgtgctcga acgggcatta aaccgcatta tgttggtggt tattgcgagc cgctttccag
    12121 aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc tgtttataca
    12181 gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt
    12241 ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag cggcagatga
    12301 ttgcggacgt caccaaaacg ctggccggag aagaagggcg gcatctggcg attgaagccc
    12361 ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt gcccgcgaag
    12421 agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt gcaggatcag atttacagca
    12481 aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgcc gcttttggtc
    12541 gtgggcgcta cgtttgcccg cgtaatctga cggcgcttgc cagcactgag cccacgcaac
    12601 aggatctgct ggcgtttctt gacgacgaac tgacgccgaa caatcaggaa gagcaaaaac
    12661 gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg cgcgatcata
    12721 ctgatatcgc tatagatgac gatctctggc gtcgtttaag taccgacaaa gccagctgcc
    12781 tcaaccgcaa ttgttactac tatcgtgaat gcccgttttt tgtcgctcgt cgggagattc
    12841 aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag
    12901 cggtattgcc tgacccgaaa aatttactgc tggtgctgga cgaaggtcat cacctgccag
    12961 atgtggcccg ggatgcgctg gagatgagcg ctgaaatcac cgcgccctgg tatcgactac
    13021 agctggactt gttcacgaaa ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca
    13081 tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagttg tatgagctta
    13141 tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag gcagagcacc
    13201 gttttgcgat gggcgaactg ccagatgaag tgctggagat ctgccagcgg ctggcaaaac
    13261 tcaccgagat gctgcgtggc ctggcggagt tatttcttaa cgatttaagt gagaaaaccg
    13321 gcagccatga cattgtacgt ctgcatcggt tgattttgca gatgaaccgc gcgttgggga
    13381 tgttcgaggc gcaaagcaaa ctctggcggc tggcttcgct ggcgcaatct tccggtgcac
    13441 cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctacacctc tggtttcact
    13501 gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt attccgcaca
    13561 ttattgtcac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg caggagatga
    13621 gtggtctgaa agagaaagcg ggcgaccgtt ttgtggcgct ggattccccc tttaaccact
    13681 gcgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc gacaacgaag
    13741 agcagcatat tgccgaaatg gcggcctttt tccgtaagca ggtggagagc aaaaaacatc
    13801 tcggtatgtt ggtactgttt gccagcggac gggcgatgca gcgctttctc gactatgtga
    13861 cggatttacg tctgatgttg ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac
    13921 tgcaccgcaa acgcgtcgcc aacggtgagc gcagcgtgct ggtgggctta cagtcatttg
    13981 ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt gcatatccac aaaatcgctt
    14041 ttccgcccat cgacagcccg gtggtgatca ccgaagggga atggctgaaa agcctcaacc
    14101 gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt taacctgatt cagcaggttg
    14161 ggcgactgat tcgaagccac ggttgctggg gcgaagtggt tatctacgac aaacgcttgc
    14221 tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg atagagcaac
    14281 cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc
    14341 ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag
    14401 agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt
    14461 atgctcatat gctcaatggt gaagtccctg accttgagtt gggcggcgta ttgattgcgc
    14521 tgcgtatcaa aggagaaggg gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc
    14581 acaccatcaa gctgacgccg ccagcaggca agccgatgcc gattgtcatc cccagttaca
    14641 acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg cacaaactcg
    14701 gttttcctgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg actgaaacca
    14761 tttttgaatt gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg
    14821 acgaacatca accggtgttt atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac
    14881 tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg aaactggcga
    14941 cgccatttgc cgaaggtgag gcgctgcgtc tttccagcgt ttcgcatccg gaatacattg
    15001 gacgcgtcgc gaagttcttt agcgatatcg gcgggcgggc gctactaatg catggtactg
    15061 aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt gaccgtgaag
    15121 ggatgcgggt gctgtatgaa aaacaggaca ctgctggtag cgagttactg ccacaagcaa
    15181 aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc gaaccgattc
    15241 ccgaatcgct gaaaatccag atggcttgct gcctggtggc tacgggtgaa gcggcaacta
    15301 tcagcgacgg cctggcgcgc gttaatcagg cattttaatt cttttctcag ccggatgagc
    15361 catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc
    15421 cacgcccggc gtgcagcata aaaatacaac aaacacataa cataaacagg agttaaccat
    15481 ggaaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg ctgtatttcg
    15541 tcagatgggt agcgaggaac aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa
    15601 cctggcaggg catgattcac atggtattgg catgatccca agctatgtac gctcctggag
    15661 tcaggggcac ctgcaaatta accatcatgc caaaaccgtt aaagaggcgg gggcggcggt
    15721 cacgctcgat ggcgatcgcg catttggtca ggtcgcggca catgaagcga tggcgctggg
    15781 gattgagaaa gcgcatcagc acggtattgc cgccgtggcg ctacataact cgcatcatat
    15841 cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct ctatccactt
    15901 tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca gccgctttgg
    15961 caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt tgcttgatta
    16021 cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt
    16081 gccgccaggt tgcctgattg acgttaacgg cgtgccgacg accaatccgg cggtaatgca
    16141 ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg cccttgcagc
    16201 gatgtgtgaa attcttggcg gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt
    16261 acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca acccggaact
    16321 cttcggcgcg ccggattgta acgcgcagac cgaagccttt gccgagtggg tgaaagcctc
    16381 gccgcatgat gatgataagc cgattttgct accgggcgag tgggaagtga acacgcgtcg
    16441 cgaacggcag aagcagggga ttccactgga tgcgggaagc tggcaggcca tttgtgatgc
    16501 agcgcggcag attggtatgc cggaagagac gttgcaggct ttctgtcagc agttagccag
    16561 ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg atcaatgagg
    16621 gttagagcat atgcgtctgt cggcaaacag acagggaaat acttgtgctg gacgtagcgt
    16681 aaacgcctga tccgtccaac ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg
    16741 catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg aattacttat
    16801 aaattaccgc agtaccgctt aatttattgt tgttggtggc ggaagtgatg ctgtatccac
    16861 tggcaccggc tgccgcggct ttctcagcca gtttcgcttc cagggcatcg agggtggatg
    16921 cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat gccgttaccg
    16981 gttccgccgc gaacacgcca aatgacagag ttgaaagagc catagcagca acaacagtat
    17041 tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc
    17101 gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt gaccgccttg
    17161 ttcaaataaa ttgattgata tctaacctac tgataaacca tttatttgtt gtacttatga
    17221 acttgtttta taccgcgtgg cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa
    17281 tacaaattat actcgctatg catctcgcca gttgtagtta acgtaactgg ctgtctttcg
    17341 aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc tgctgacaat
    17401 gaatacataa gcgcacgcca ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc
    17461 actcttcaca ttcatccagg ctttcgccgc gcggaatttc accccgagcg cgggcaatcg
    17521 catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcgttagcc caaccggatg
    17581 ccatatcgac ctccccatat caatacttgt acagttaagt gtagctaatc cagggacgaa
    17641 ctcgggcagt tcaagcatca gatctccgac cattcccgca gcagattatg ataaagatta
    17701 agcagcgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg aatattgttg
    17761 tccagttcaa acagcatggc gcgcttttta tcatcgcgga tcatcgactg gatccacata
    17821 aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc aatgcaggct gctggaggga
    17881 tacaacacga gatcgcctgc cgggagtttt acccgatgtt gtccgaaggt gtcattaacg
    17941 accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca
    18001 gtacgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg gaaaccatag
    18061 gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg
    18121 gcaaagaata aagcatgttg gttaaccgcg ttcagcacct cattttgcag ggcggcgtat
    18181 aacgtgctgc gggtgtcgac ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg
    18241 gtgacgcgtc catccaccca ttcggcttgt tccagttgtt cgcgaaaacg agcgacgtcc
    18301 tgtggcgata acacgccggg aatgtggtac atcatacaat ttctccaaaa agtggggcct
    18361 gcgccccaca tctgaatcag aaatgcatat tggctgtgag caagaaggtt cttggctcgc
    18421 ccgggtgata acggtagccg ctcttgttga ttgaggcgac gtaatcggta tcaaacaggt
    18481 tgtaaacgtt tagctggaag tcgagattgc gattaactcg ataccccagt ttggcatcgg
    18541 cgacccagta accttcggta aacgctggcg ttcccaccgc gccgtctgaa cctttatgca
    18601 tactgccgat atagcgtgcg cccgcgccaa cagagatatc gtcggttgcc tgatattggc
    18661 tccataaggt gaaggcgtgc tccggggtat acggcagcga tgaggaacca tcctgggcaa
    18721 catctttgcc gtttttgatg gttgcttttt gctgggtata gccgccaatc acctgccacg
    18781 cgggagtgat attcccggcc acggatatct catagccttc gacgcgtttc ttaccgtatt
    18841 gcgagtaagt tccgtcatca ttttgctcaa cttcattttc gatatcagta cggaacagcg
    18901 cggcggtgag caacagacgt ttatccagaa cctgccattt ggtgccaatc tcgctggtgt
    18961 tggctttttg cggtttaaaa tcggtgcggt tggcactgtt accgctgcca gactgcgcaa
    19021 gggcgaagtt gttgccgccc ggaggctgct gggaaacggc atagttaata tagacattgc
    19081 cgttttccgt caggtgatac agcgccccgg ctttccagtt catcagattg cccgacttgg
    19141 cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc agttgggcag gtgatggcac
    19201 cgcgtccgct gccgccgcag gcggtggcac tgtcatattc agtatgataa ttatccagac
    19261 ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg
    19321 cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa
    19381 tgctgctgtc aggatgataa atatttaccg cgggtaacgt caccggatta acgccgtagt
    19441 tagtctgcgt ttcacgggta aattccacgc cggtactgac atcatgaccg atagaaccgg
    19501 tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttattactc acatctttgg
    19561 tattcgccgt gcgtgaccag gtccagctat tcacatcgct ggtgggctga gtaatattcg
    19621 acgccccgcc cataatcgcc gtcatcaggt aatcctgctt tacgcgcgac caacgggtag
    19681 tattgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg
    19741 tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca acttttccgg
    19801 aatgattcag agccgctgtt cccgcagatg gggcagaata acccggcaaa ccgatggtcg
    19861 gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag ataattaaga tacaaacgat
    19921 tcgctgtacc aaggccaaaa gcgatagaag gggcgacgcc gtaacgctca tttttgactt
    19981 tgtcgcgtcc ggcatcgtgc gttttttcgc ccatcacatt caggcgcacc gcagtggtat
    20041 caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg
    20101 cggaggcgtc aatgccggaa tcattgcgcg gctgcttgct gatcatattg atcgagcccg
    20161 tcggcgcgct gcgcccgtag tcggtgccgg aaggcccttt aatcacttcg acctgctcag
    20221 tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcaata taaatactgt
    20281 tagaggtatc agcaccgcgc atataaatgg cgtcgccagt ggtggagtta ccgttctcac
    20341 ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc gtcggtaagg ttggttgcgc
    20401 cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt gcgggtagta tccgctaccg
    20461 gacgcgagaa tttcggatcg gcagattgtt gtggcgcata aagcgaaggc gttgatgctt
    20521 cgacaaccag cgtgtcatcc gcgttagttt gcccttcggc ggcgagtgcc tgagccacag
    20581 gcgtgatgcc aatacaaaga ccggcaaaga acgtgagcga atgaaattgt ctggcaggga
    20641 aattgcgatt gttttccatt ttgcaggtga ctttttttta tatgttgaaa ttttcactgc
    20701 cacgagaaag cctctctgcg gatgcgatgc cactgttaat ctcgtgatag tgttcgtgca
    20761 agtgataata attttgataa gcattatcac ttggcgcgat tttctatcac ccattgcgcg
    20821 aaaaagaaat acatttattt acattgcgtt catatttgat gttttttgat tacaaaacag
    20881 gcgaaaactg catccagagg cgaatgcagt tacgttggtg gggttatttg tagatggttg
    20941 cagtaccgaa catctggtca tttccgcctg cggaattaat gacataacct ttcgcgcctt
    21001 gctcgcgcgc tttttctgcc agtttatcct ccagatcgct taggtttgat gcgccagttg
    21061 ccgaaacggt accggcgggc cgtagctgac cgctgtccag gttactcatc ggttgcgcgg
    21121 cataagccgt gagagagatt ccgctcagga cggtggcaat cagtagtgtg aggcactttt
    21181 tcataatgac atcctcataa atacaacggg tagtgcctga taagtgtagg tcgcctgggc
    21241 gatgaaaatg acgcaaaaaa ttgacagaga tgtgcttatt ttttgaacga tatattttta
    21301 caaaataaga caaatcaatg acatgcataa tgctgatttt tatgatgaat tgccataaac
    21361 catagccatg ttaaggtttt ggggaatgat aaacgcagcc agatatttac cctgtcgcgt
    21421 tatgcgggta tcttacgccg cagcttaaag gagaatgcta tgtccgccca gaaaccgggg
    21481 ttgcatccgc gcaaccgtca tcacagccgc tacgatctcg ccacgctttg tcaggtcaat
    21541 cctgaactca ggcaattcct cacgcttaca cccgccgggg agcaaagcgt agactttgcc
    21601 aatccgctgg cggtgaaggc gctcaataag gcgttgctgg cccattttta cgccgtagcg
    21661 aactgggata tccccgacgg ttttctctgc ccaccagtac cgggccgggc ggattatatt
    21721 catcaccttg ccgatttact ggcagaagca agcggaacaa ttccagcgaa tgccagcatt
    21781 ctggatatcg gcgttggtgc gaactgtatt tatccgctga ttggcgtaca tgaatatggc
    21841 tggcgtttta ccggtagtga aaccagcagc caggcgttaa gcagtgcgca ggcgattatc
    21901 agttctaatc cggggcttaa ccgcgccatt cgtctgcgtc ggcaaaaaga gagtggggcg
    21961 atttttaacg gcatcatcca taaaaacgag caatacgacg cgaccttgtg taacccgcca
    22021 ttccacgatt ccgctgctgc ggcacgggca ggtagtgagc gtaaacgccg taacctgggg
    22081 ctgaacaaag acgatgcact gaactttggc ggccagcaac aggagttgtg gtgtgaaggc
    22141 ggtgaagtca cctttatcaa aaagatgatt gaagagagca aaggcttcgc gaagcaggtg
    22201 atgtggttta catcactggt atctcgtggt gaaaacttac cgccgttgta tcgtgccctg
    22261 acggacgtgg gcgcggtgaa ggtggttaaa aaagagatgg cccaggggca aaagcagagt
    22321 cgctttattg cctggacctt tatgaacgac gagcagcgcc gccgttttgt caatcgccag
    22381 cgttaaagcg ttggttcccc cggcggtaac ggttcagccg gggtcgcgcc cggagcaggc
    22441 agcacctgat aagtctgcac tggcgcgcga acgcccgcca ggtcgaaatg ttttttcacc
    22501 tggctgtcga gggcaaagcg taccgtccac tgtttgagtg gcagcgtggt gaacgaaaca
    22561 cgcagtgtaa acgcggtatt gcttaagccg acaatcccgg caaaattcgg ttcaccaata
    22621 atcagcccgc gaatttcttc gttttccatt aattccgcta ccgcatcttt cagtgcctga
    22681 ttggctttat cagcatcttc atggcgatca acatcataat ttgccaccac cgaaccaatg
    22741 ccgcggacaa agttagcaaa ggtggttatc gaagaccacg gaatgatgtg atacgccccg
    22801 gtatcctggc gtacgcccac ggagcgaatc gacatccgtt ccactgtgcc ggtcaacggc
    22861 ccgatagtca ccaaatctcc agtgttcatg ccgttttcaa actgaataaa taccccggtg
    22921 ataatatctt tcaccagcgt ttgcgaacca aacgagatag ccagacctaa tgccccggca
    22981 cctgccagca atggcgcgat attgacgccg atttccgaca acacaatcat gatggtgatg
    23041 gtactgataa tcaccgccag cgcgttacga aacagcgtaa gcagggtacg cgtgcgggcg
    23101 ctgggtagcg ggcggccatg aatatccgaa gccagccggt tttcgatcaa actggcgagc
    23161 accgtccagc caaccgccga gaagaaaaga atgagtgcga tacggatcag gatatctacg
    23221 gttttctgcc ccgcgccgtt ttgcagccag ttccagaaat cgaacaatcc ccatgcgctc
    23281 aacagcaaca ttaccgccac gcagactgtc agaatacgcg ccgttttcag cgccgccgac
    23341 agccagccat tcaaccgttt ttgcagctcc ggatagttac gctgagtatg tggcgagaga
    23401 gtgatggttt tcgccagcca gcgggaaaac ataccggaaa caaacgctgc gataccaata
    23461 atcgccaggc tgcgcaccgt tgcacccatc ataaatttca ggctgttgcc cggatcgaac
    23521 aacgaaaaga aaaacagcac gataaaatag gcgcttgcca gccagtgcca caccagcgca
    23581 aaggcgcgga taaacaggct gaaaaaggcc agcgaatgct ccgcgaagtt gagcaaatgc
    23641 tgggtaatct cttttttatt acgaaagatc aggtacaacg cccagacggt catgcacagc
    23701 ataatgatga cgttcgccag cgcacctatc tgtacattca cctgattaga gataatcggc
    23761 acggccacaa tcaggccata acctatcaaa ctgcttaacc agctcaggcg acgactccag
    23821 taacgggcgc tctcgtcctg aatcgtgaac gggcgcagct ccgccacgtt tgggcaaaaa
    23881 atcaggcgta gtacggcttt gaaaaattca atgagggcaa aggcgttgag aaacaaactt
    23941 tgttggaaag cgatggtgcg actgcctgca ttcaggttgt cgcttaatac ctggccgaca
    24001 aataatgtca gggccagtaa cagcaggtcg ataataaacg ccccgataat catcgccgga
    24061 agctgcaacc agttgctgcg ctcacgattt ttttgccgcg cccactgacc cattttgcga
    24121 tacagcggca gtgcgcacag gcgaatcagc cagtaaaaac caaacactaa taccgctaac
    24181 attgaaaaat gggtcagcgc attgctgaag gtttgtggat taaacggctt atgcggggag
    24241 ccggtgatat tgcgataaag ttgcccgaag cgggcggaaa gggcttcacc ataatggcgg
    24301 ctgacttcgg tgaccttttg cagcacggtt tgctcttcga ccagcgtcgg cggcactatt
    24361 tttggtaccg gttcagcagg gggcgtagcg gcaacggtgc gcaactggtc gatcaactct
    24421 ttacgcgagg tgtcattatc cagcacatcc gccagtgcgc cataggccgc ttttttttgt
    24481 tcgatatccg gttccggggc cggttcagtc gttgagtccg ttgtcgttgt ggttgtaacg
    24541 ccgggtatgg ataccgcgtg ggcaggtgcg cccagcaggc agaagaggat gaacaggatc
    24601 caccgcatga ctcctccagt gagaaaatag agcaaaaaga taagtataga tgctggagga
    24661 ggggcgattt taaatgagag gaatctggtg tgcctccctt tcgggtgaaa gggaggaagg
    24721 gatattaagc aggttgctga caacgtgcgg gcttttttgc cggatgcggc gtgaacgcct
    24781 tatccgtcct acaagttcat gcaattttaa taagttgcca gatatcctgt aggcctgata
    24841 agcgtagcgc atcaggcaat gtgtcttatt aagagacgtg ctgcaaaaat tcctgcaagc
    24901 gctggctcgg cgggttcttg atcaacacct gcggattgcc atcttccgca atccggcctt
    24961 tgtcgataaa gatcagccgc gaagctactt tctcggcaaa accgatttcg tgggtcacga
    25021 tcaccatcgt catcccttct tcagccagat cctgcataac cttcagcact tcatggcgca
    25081 gttccgggtc aagagcggaa gtcggttcat caaacagcat cattttcggc ttcaccgcca
    25141 gcgcgcgggc aatcgccaca cgctgctgtt gaccaccaga aagttcggaa gggtagtgat
    25201 gtgcacgttc tgccagaccg actttcgcca gcagctcacg tgccagtttt tccgcctctt
    25261 ctttgttcgc gccacgcacg cgtagcggac caaacatgac gttttccagc gccgtcagat
    25321 gcgggaagag gtaaaactgc tggaacacca tacccgcttc ctggcgaatc aggcgctcgt
    25381 caactttcgg atcgttaacc ttcaggccat cgacaatcag atcgccggag gtgatttctt
    25441 ccagtttgtt gatgcagcgc agcagggtcg atttaccgga accggacggc ccgataatca
    25501 ccacgacttc gccctgagca atgttcaaat cgatattgtg cagcacctgg gttggaccaa
    25561 aatgcttgga gacgttttta aattcaatca caggattttc atccttcttt ccagacgacg
    25621 cagaataaag ctcagcacca gggtaataat cagatagaac accgccacgg cgctccagat
    25681 ctcaagggcg cggaagttac cggcaataat ttcttgcccc tgacgggtca gttccgccac
    25741 gccgataaca ataaacagcg aggtgtcttt aatgctgatg atccactggt tacccagcgg
    25801 cggcagcata cgacgcagtg ccagcggtaa aatgacgtag cgaatggttt cccaacgtga
    25861 aagaccgagc gccagtcctg cttcacgaaa acctttgtga atagacagca ccgcaccacg
    25921 cgtgatttcc gcaatgtacg cgccggagtt gatcatgatg gtgaccaccg ccgcagtaaa
    25981 tgggtcgatg cgtaagtcgt taaacgccat cggcagggcg aaatagataa acatcacctg
    26041 gacgacgata ggtgtgccgc ggatcacttc aataaagacc agcgcgacgt ggttggctat
    26101 ccaaccaccg aaggtgcgtg caaaacctgc cagcaatccg attaccagac cgcctgccag
    26161 accgaggacc gaaatccaca gggtcatttt ggcaccttca atcagaagcg gaatggcagg
    26221 ccagatggca ctccagtcaa actgcatatg ttgttcctgt taccgtggtg taaataatca
    26281 aattcagggt aattaatggc cccggagcgg gtttgcgccc ctcaccctaa tcctctcccc
    26341 ataggggaga gggaactgcc agtgcgtttt acaggtgtag cgttattatt tcggttcagt
    26401 accgaaccat tttttgtaga tttcgttgta agttccgttc tcgcgcaggg ttttcaacgc
    26461 gccgttgact ttgtcacgca gctcgtcgct acctttcggg aacgcaatac cgtattgctg
    26521 cgcttccaga gagtcaccta ccgctttgaa ctgaccgtta ccggcggttt tgatgaagta
    26581 cagaatgttt ggcgtatcgt gcagaacggc gtctgcgcgg ttggtgccca gttccatata
    26641 ggcgttatcg atgttcggga actgacgcag atctttagtt ttgatgtttg ctttcgcgta
    26701 atcaacggag ccagtaccgc tcttcacagc aaccactttc ccgtcgagat ctttcacgct
    26761 tttcacatcg ttattgttag ctttcaccat cactaacagg ccgcttttgt agtagccgtc
    26821 agagaaatcg atcgcttttt tacgctcgtc ggtgatggta atgcccgcca gcgccagatc
    26881 gacgtttttg gtttgcagtg ccggaatgat cccactgaaa tccatcggct tcagttcgta
    26941 atccagcttc agctctttag cgatggcagc ccacagatca acgtcaaagc ccacatattt
    27001 atcgccctgt ttaaattcaa acggaacgaa ggcggtatcc gtcgcgacaa ctaatttttt
    27061 atccgcggca tgagaagaaa ccgcaaaagc cagggtcagt gcagccagtg aaacttttaa
    27121 tacagacttc atagcatttc cttttttcta tccacgggac gatcccctgc gtgacattca
    27181 catatatgaa aaaatcgtgc cagttttgca actcattgat ttttcgagat gcggcaaaaa
    27241 cgtgatgcac gatttatagg gcaataccct gaagatgcac cattctgggg caccaatctg
    27301 gtgcgctaaa attgtgcact caacacagtg attatttagc gtaaaacctg atgaaaaaac
    27361 aatctttatg taacgattgt gtgatgatgt ggatacaaaa aatttactta atcagctgga
    27421 gatagcagat ggatgcacta aataagtgcg ttgaggtggg ctgcaaaaca aaacggcctc
    27481 ctgtcaggaa gccgctttta tcgggtacta aagttctgca ccatcagcga tggatttatt
    27541 cgatgttaga ctcgataaac cacaggaatt tatccaggtc gcgagacgcg gcggtcagga
    27601 tatctgcggt gtcgtcatct ttcgcttcgc caatcgcttt gcgtacgtca ttagcgacga
    27661 ttgcgtaacg gtcagccagt tctttcaggt gatcctgaac gttgtggatg tccagcgggt
    27721 aacttttcag cggggttttg ctgttgataa cttgagtggt ccccagagct acaccgccca
    27781 gctgcactgc acgttctgcc atggtatcca gatgatcgat cagtgcggtg cggaagccat
    27841 ccagcatttc atgtacggca atgaagttag cgccgcgcat gttccagtgc gcttgtttgg
    27901 taatcaaaga aagatcaata aactggataa cctggcgatt cagcaactct actgttgctt
    27961 ttttctcgct gtcggagaca tcgttgcggg tataaagcag attggtcgct tttgatttaa
    28021 ctaatttagc ggtactcata atttcatatc ctcttgatgt tatgtcccag taattaacga
    28081 gactaagtat agcaccggct atgtgttccg ctattctggc tgttcctatc acactaatag
    28141 tggtaacaag cgtgaaaaac
//
LOCUS       DAPHJE010000058        27411 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPHJE010000058 DAPHJE010000000
VERSION     DAPHJE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.058"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..1295)
                     /gene="icd"
                     /locus_tag="RH222_002489"
     CDS             complement(45..1295)
                     /gene="icd"
                     /locus_tag="RH222_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV3797491.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1467..2120
                     /gene="rluE"
                     /locus_tag="RH222_002490"
     CDS             1467..2120
                     /gene="rluE"
                     /locus_tag="RH222_002490"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV3797492.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2130..2591
                     /gene="nudJ"
                     /locus_tag="RH222_002491"
     CDS             2130..2591
                     /gene="nudJ"
                     /locus_tag="RH222_002491"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV3797493.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2645..3751
                     /gene="mnmA"
                     /locus_tag="RH222_002492"
     CDS             2645..3751
                     /gene="mnmA"
                     /locus_tag="RH222_002492"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV3797494.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3787..4428
                     /gene="hflD"
                     /locus_tag="RH222_002493"
     CDS             3787..4428
                     /gene="hflD"
                     /locus_tag="RH222_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV3797495.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4432..5802
                     /gene="purB"
                     /locus_tag="RH222_002494"
     CDS             4432..5802
                     /gene="purB"
                     /locus_tag="RH222_002494"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV3797496.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5971..6642
                     /gene="phoP"
                     /locus_tag="RH222_002495"
     CDS             5971..6642
                     /gene="phoP"
                     /locus_tag="RH222_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV3797497.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6642..8102
                     /gene="phoQ"
                     /locus_tag="RH222_002496"
     CDS             6642..8102
                     /gene="phoQ"
                     /locus_tag="RH222_002496"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV3797498.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8178..9299
                     /gene="roxA"
                     /locus_tag="RH222_002497"
                     /gene_synonym="ycfD"
     CDS             8178..9299
                     /gene="roxA"
                     /locus_tag="RH222_002497"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV3797499.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9348..10574)
                     /gene="pepT"
                     /locus_tag="RH222_002498"
     CDS             complement(9348..10574)
                     /gene="pepT"
                     /locus_tag="RH222_002498"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV3797500.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10824..11960
                     /gene="potA"
                     /locus_tag="RH222_002499"
     CDS             10824..11960
                     /gene="potA"
                     /locus_tag="RH222_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV3797501.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11944..12801
                     /gene="potB"
                     /locus_tag="RH222_002500"
     CDS             11944..12801
                     /gene="potB"
                     /locus_tag="RH222_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV3797502.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12798..13592
                     /gene="potC"
                     /locus_tag="RH222_002501"
     CDS             12798..13592
                     /gene="potC"
                     /locus_tag="RH222_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV3797503.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13589..14635
                     /gene="potD"
                     /locus_tag="RH222_002502"
     CDS             13589..14635
                     /gene="potD"
                     /locus_tag="RH222_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV3797504.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14693..15154
                     /locus_tag="RH222_002503"
     CDS             14693..15154
                     /locus_tag="RH222_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDV3797505.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15151..15939
                     /locus_tag="RH222_002504"
     CDS             15151..15939
                     /locus_tag="RH222_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDV3797506.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16078..16899)
                     /gene="cobB"
                     /locus_tag="RH222_002505"
     CDS             complement(16078..16899)
                     /gene="cobB"
                     /locus_tag="RH222_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV3797507.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16915..17826)
                     /gene="nagK"
                     /locus_tag="RH222_002506"
     CDS             complement(16915..17826)
                     /gene="nagK"
                     /locus_tag="RH222_002506"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV3797508.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17855..19099)
                     /gene="lolE"
                     /locus_tag="RH222_002507"
     CDS             complement(17855..19099)
                     /gene="lolE"
                     /locus_tag="RH222_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV3797509.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19099..19800)
                     /gene="lolD"
                     /locus_tag="RH222_002508"
     CDS             complement(19099..19800)
                     /gene="lolD"
                     /locus_tag="RH222_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV3797510.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19793..20992)
                     /gene="lolC"
                     /locus_tag="RH222_002509"
     CDS             complement(19793..20992)
                     /gene="lolC"
                     /locus_tag="RH222_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV3797511.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21254..22327
                     /locus_tag="RH222_002510"
     CDS             21254..22327
                     /locus_tag="RH222_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3797512.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22455..25901
                     /gene="mfd"
                     /locus_tag="RH222_002511"
     CDS             22455..25901
                     /gene="mfd"
                     /locus_tag="RH222_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV3797513.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26045..27007
                     /gene="ldtC"
                     /locus_tag="RH222_002512"
     CDS             26045..27007
                     /gene="ldtC"
                     /locus_tag="RH222_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV3797514.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27090..27347)
                     /gene="bhsA"
                     /locus_tag="RH222_002513"
     CDS             complement(27090..27347)
                     /gene="bhsA"
                     /locus_tag="RH222_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV3797515.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6682 a         7110 c         6934 g         6685 t
ORIGIN      
        1 cgggcgtgtt atacgcccgt ttcaatattt aacacatgga gagattacat attcttgatg
       61 atcgcgtcac caaactctga acatttcagc aatttagcgc cttccatcag acgttcgaag
      121 tcataggtta cggtcttggc attgattgcg ccttccatac ctttaacaat caggtcagcc
      181 gcttcagtcc aacccatatg gcgtaacatc atctcagcag agagaataat agagcctggg
      241 tttactttgt cctgaccggc atatttcggc gcagtaccgt gggtggcttc aaacagggcg
      301 cattcgtcac cgatgtttgc acctggggcg ataccgatac cgccaacctg cgctgccagg
      361 gcgtcagaaa tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga
      421 cgcagcagga tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg
      481 ccggtgttcg ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac
      541 tcttcacgcg ccagctggta gccccagtct ttaaacgctc cttcggtgaa cttcatgatg
      601 ttgcctttgt gcaccagagt cacagagtca cgatcgttag caattgcgta ttcgatcgct
      661 gcacgaacca gacgtttggt gccttcttcc gaacacggct taataccgat accacaatgt
      721 tccgggaagc gaattttctt cacccccatc tcttcacgca ggaatttaat cactttctcg
      781 gcgtcggcag agtctgcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg
      841 aagataacca tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ctgatagtaa
      901 cgtaccggac gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag
      961 cgaataccgc caccaacagg agtggtcagc ggacctttaa tggcaacgcg atattcacga
     1021 atcagatcaa gggtttcagc aggcagccag acatcctgac cataaacctg tgtggatttt
     1081 tcaccggtgt aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact
     1141 gcagcgtcga ccactttcag catggctggg gttacatcta caccgattcc atcaccttca
     1201 atgtaaggga taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc
     1261 ttgccttgtg ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg
     1321 tttgctcgtc tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga
     1381 gcaatttttt gttaatgatt tgtaattggc ttgtcaatac taccctattg tttgccgtca
     1441 tgaaagatgc gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc
     1501 aaaaaacttc ttttagaaat caccaggtta agcgattcag ctcgcaacgt tctaccaggc
     1561 gtaaacctga aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc
     1621 cgcagttcac cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg
     1681 tttatgcagc aggtcgcctt gaccgcgata gcgaagggtt gctggtgctg accaataacg
     1741 gcgcgttgca ggcgcgttta acccagccgg gtaaacgcac cggaaaaatc tattatgtgc
     1801 aggtggaagg tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa
     1861 atgatggccc taccctgccc gcaggcgcgg agctggttga cgaacccgcg tggttatggc
     1921 cgcggaatcc accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct
     1981 tatatgaagg acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc
     2041 tgcgactgat tcgctatgcg atgggagatt actctttgga taatcttgcc aatggcgaat
     2101 ggcgagaagt gacagattaa ggatgcaaaa tgtttaaacc gcacgttacc gttgcttgcg
     2161 tggtgcacgc agaaggcaaa tttttagtcg ttgaagagac gattaatggt aaagcgttat
     2221 ggaaccaacc tgccgggcat ctggaagccg atgaaacctt agtggaagcc gccgcccgtg
     2281 aactgtggga agaaaccggc atcagcgcgc agccgcaaca ctttattcgt atgcatcagt
     2341 ggattgcgcc agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa
     2401 tatgcccgac ccagcctcat gacagcgata tcgactgctg ccgttgggtc agcgccgaag
     2461 aaattttaca ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatt cgttgttatc
     2521 aaagcgggca acgttatccg ctggagatga ttggcgattt taactggcct tttacaaagg
     2581 gtgtcatcta aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga
     2641 tccaatgtct gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc
     2701 cgtttctgcc tggctgttgc aacaacaggg atatcaggtc gaaggcctgt ttatgaagaa
     2761 ctgggaagaa gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca
     2821 ggctgtctgc gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg
     2881 ggacaacgtc ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga
     2941 tattctgtgc aacaaagaga tcaaatttaa agccttcctc gaatttgccg ccgaagattt
     3001 aggtgccgat tatatcgcta ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag
     3061 ccgcctgctg cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag
     3121 ccatgagcag attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg
     3181 taagattgct gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg
     3241 cttcattggc gaacgtaaat tccgcgagtt cctgggccgt tatctcccgg cgcaaccggg
     3301 caaaatcatt accgtcgatg gcgatgaaat tggtgagcac caggggctga tgtatcacac
     3361 tctcggtcag cgtaaaggtc tgggtatcgg tggcaccaaa gaaggtaccg aagaaccgtg
     3421 gtatgtggtg gacaaagacg tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca
     3481 cccgcggctg atgtctgtcg ggttgattgc ccagcagttg cactgggtcg atcgcgaacc
     3541 attcaccggc actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccttg
     3601 caccgtcaag gcgctggacg atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc
     3661 cgtgacgccg ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat
     3721 tattgagcag cgtctgccgc tgccggtctg attattatct ttacttaaaa caaggaagca
     3781 gtgaacgtgg caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg
     3841 gcacgcctgg tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc
     3901 tcactcaaca gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc
     3961 gaagccaacc tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc
     4021 cagggcttaa acgccgaatt aacccgctac acactcagct tgatggtgct tgagcgcaaa
     4081 ctctcctcag cgaaaggcgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc
     4141 cagctcgaac acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat
     4201 gttgatgtga ttagcccgct tggcccgcgc attcaggtca ccggttcccc tgctgtactg
     4261 caaagcccac aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc
     4321 gtgctctggc accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg
     4381 accactcagg caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta
     4441 tcctcactga ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg
     4501 cgcgggattt tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg
     4561 ctgcaaaaac tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca
     4621 atcggttacc ttgatgcaat cgtcgccagt ttcagcgaag aagatgcggc gcgcatcaaa
     4681 actatcgagc gtaccactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa
     4741 gtggcggaga tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg
     4801 gaagatatca ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc
     4861 ctgccatact ggcgtcaact gattgatggc attaaagatc tcgccgttca gtatcgcgat
     4921 atcccgctgc tgtctcgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag
     4981 atggcaaacg tcgcctaccg tatggagcgc cagtaccgcc agcttaacca ggtggagatc
     5041 ctcggcaaaa tcaacggcgc ggtcggtaac tataacgccc acatcgccgc ttacccggaa
     5101 gttgactggc atcagttcag cgaagagttc gtcacctcgc tgggtattca atggaacccg
     5161 tacactaccc agattgaacc gcacgactac attgccgaac tgtttgattg cgttgcgcgc
     5221 ttcaacacca ttctgatcga ctttgaccgt gacgtctggg gttatatcgc ccttaaccac
     5281 ttcaaacaga aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac
     5341 ccgatcgact tcgaaaactc cgaagggaat ctgggccttt ccaacgcggt attgcagcat
     5401 ctggcaagca aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg
     5461 cgtaacctcg gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc
     5521 gtgagcaaac tggaagtgaa ccgtgaccat ctgctggatg aactggatca caactgggaa
     5581 gtgctggctg agccaatcca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag
     5641 aagctgaaag agctgactcg cggtaagcgc gttgacgccg aaggcatgaa gcagtttatc
     5701 gacggtctgg cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggctaac
     5761 tatattggtc gagctatcac gatggttgat gagctgaaat aaacctcgta tcagtgccgg
     5821 atggcgatgc tgtccggcct gcttattaag actatccgct ttttattttt tcactttacc
     5881 tcccctcccc gctggtttat ttaatgttta cccccataac cacataatcg cgttacacta
     5941 ttttaataat taagacaggg agaaataaaa atgcgcgtac tggttgttga agacaatgcg
     6001 ttgttacgtc accaccttaa agttcagatt caggatgctg gtcatcaggt cgatgacgca
     6061 gaagatgcca aagaagccga ttattatctc aatgaacata taccggatat tgcgattgtc
     6121 gatctcggat tgccagacga ggacggtctg tcactgattc gccgctggcg tagcaacgat
     6181 gtttcactgc cgattctggt attaaccgcc cgtgaaagct ggcaggacaa agtcgaagta
     6241 ttaagtgccg gtgctgatga ttatgtgact aaaccgtttc atattgaaga ggtgatggcg
     6301 cgaatgcagg cattaatgcg gcgtaatagc ggtctggctt cacaggtcat ttcgctcccc
     6361 ccgtttcagg ttgatctctc tcgccgtgaa ttatctatta atgacgaagt gatcaaactg
     6421 accgcgttcg aatacaccat tatggaaacg ttgatacgca ataatggcaa agtggtgagc
     6481 aaagattcgt taatgctaca actctatccg gatgcggagc tgcgggaaag ccataccatt
     6541 gatgtactga tgggacgtct gcgcaaaaaa attcaggcac aatatccgca agaagtgatt
     6601 accaccgttc gcggccaggg ctatctgttc gaattgcgct gatgaaaaaa ttactgcgtc
     6661 tttttttccc gctctcgctg cgggtacgtt ttctgttggc aacggcagcg gtagtactgg
     6721 tgctttcgct tgcctacgga atggtcgcgc tgatcggtta tagcgtcagt ttcgataaaa
     6781 ctacgtttcg gctgttacgt ggcgaaagca atctgttcta tacccttgcg aagtgggaaa
     6841 acaataagtt gcatgtcgag ttaccagaaa atatcgacaa gcaaagcccc accatgacgc
     6901 taatttatga tgagaacggg cagcttttat gggcgcaacg tgacgtgccc tggctgatga
     6961 agatgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
     7021 acgataccag cctcttgctg agtggagatc attcgataca gcaacagttg caggaagtgc
     7081 gggaagatga cgacgacgcg gagatgaccc actcggtggc ggtaaacgtc tacccggcaa
     7141 catcgcgaat gccaaagtta accattgtgg tggtggatac cattccggtg gagctaaaaa
     7201 gttcctatat ggtctggagc tggtttatct atgtgctctc agccaatctg ctgttagtga
     7261 tcccgctgct gtgggtcgcc gcctggtgga gtttacgccc catcgaagcc ctggcaaaag
     7321 aagtccgcga actggaagaa cataaccgcg aattgctcaa tccagccaca acgcgagaac
     7381 tgaccagtct ggtacgaaac ctgaaccgat tgttaaaaag tgaacgcgaa cgttacgaca
     7441 aataccgtac gacgctcacc gacctgaccc atagtctgaa aacgccactg gcggtgctgc
     7501 aaagtacgct gcgttctctg cgtagtgaaa agatgagcgt cagtgatgct gaaccggtaa
     7561 tgctggagca aatcagccgc atttcacagc aaattggcta ctacctgcat cgtgccagta
     7621 tgcgcggcgg gacattgctt agccgcgagc tgcatccggt cgccccactg ctggacaatc
     7681 tcacctcggc gctgaacaaa gtgtatcaac gcaaaggggt caatatctct ctcgatattt
     7741 cgccagagat cagctttgtc ggtgagcaga acgattttgt cgaggtgatg ggcaatgtgc
     7801 tggataatgc ctgtaaatat tgcctcgagt ttgtcgaaat ttctgcaagg caaaccgacg
     7861 agcatctcta tattgtggtc gaggatgatg gacccggtat tccattaagc aagcgagagg
     7921 tcattttcga ccgtggtcaa cgggttgata ctttacgccc tgggcaaggt gtagggctgg
     7981 cggtagcccg cgaaatcacc gagcaatatg agggtaaaat cgtcgccgga gagagcatgc
     8041 tgggcggtgc gcggatggag gtgatttttg gtcgccagca ttctgcgccg aaagatgaat
     8101 aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt gcagatacca
     8161 gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat tttcttgaac
     8221 gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt attgacccga
     8281 tctctccaga cgagttggcg ggtctggcga tggaaagcga agtcgacagt cgactggtca
     8341 gtcaccagga tggcaaatgg caggtcagcc acggtccgtt cgaaagctac gatcatctcg
     8401 gtgaaactaa ctggtcattg ttagtgcaag cagtaaatca ctggcatgag ccgaccgccg
     8461 cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg atgatctctt
     8521 tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg tttatcattc
     8581 agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa cagcactgcc
     8641 cacacccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa gagctggagc
     8701 ctggcgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg ctggaaaatg
     8761 cgatgaacta ttccgtgggt tttcgcgcgc caaatacgcg ggaattaatt agcggatttg
     8821 ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatccg gatgttccac
     8881 ctcgcgctca tcctgcggac gttctgccgc aagaaatgga taaactgcgt gagatgatgc
     8941 tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt atatcccagt
     9001 cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat gaaatctacg
     9061 atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta ttgcgcattg
     9121 gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg gcactggatg
     9181 cactcgccag caacattgcg ctgaccgcgg agaattttgg cgatgcgctg gaagatccgt
     9241 cattcctcgc gatgctcgcg gcgctggtca atagcggata ctggttcttc gaagggtaag
     9301 tttggattta agccggatgc ggcatcacat gccgcatccc ttttcgctta cttccgttgc
     9361 gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc ttccagagtc
     9421 acaaactcat gcttaccatg atagttgtaa ccgccagtga acaggttcgg gcacggtaat
     9481 cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag ttccggttca
     9541 atatcgcagt cacgcatcgc ctgctgggcg atatcgagaa tatgcggatg ctcaaccact
     9601 ttctcgcgca tattgtagta actgtcttca atcaccagtt caatgtagca atcaggatgt
     9661 aaccctttgc ccactttttt ggcgatctcc atcattttac gtttacgcgc ttcaaactgt
     9721 ttacggtcga aatcacggat gatgtagtgc atatcggctc ggtcaacggt gcctttcatg
     9781 ctcgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc atccgccgga
     9841 acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc tttcgccgtg
     9901 cccggatgaa cattgttacc gacaattttg atattgaccg atgcggcgtt gaagttttca
     9961 aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc atcgaaggca
    10021 tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa ggcgacgcga
    10081 atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat gatttctgca
    10141 ataccggctt tgtcatcggc acctaacaag gttttaccat cggtggtaat caacgtctga
    10201 cccagtagct gatgcagcac cgggaacata accggtgata aaacttcatc gccgataccc
    10261 agcgcaatat cgccaccgcg atagctttca acaatttgcg gattaacatt tttgccgctg
    10321 caatccggtg aggtatccac atgagaaata aagccaatcg ccgggatatc gccagggacg
    10381 ttagccggta acgtcgccat caaagtgccc ttctcactta aggtcacatt gataagcccc
    10441 atttcttcga gctgctcttt cagcagatgc aataacttcc attggccttc cgtgctggga
    10501 acctgtctca cccctgcttt tgattgggta tccagagaca cgtagttcaa aaatcgctca
    10561 agtagtttat ccatgtagtc accctcactt tttgtgacaa cattattaag aagcaagaaa
    10621 agacaaatat tgcgtcaggt cacttttacc cctgcaagcg ggaatattta tcagcattac
    10681 cttaatgaat ataaagctaa gccagtaatt cacaacaaag attggcgatt caagcggttt
    10741 gccgtagaat taccgccctc attaagagtc accaaggtgg ttaaccacaa accccgcatc
    10801 ggtaagccat ccgttgcgtt tacatgggac agagtaaaaa attgaataaa caaccgagtt
    10861 cgctttcacc gctggtgcaa ttggcgggaa ttcgcaaatg ctttgatggt aaagaggtca
    10921 ttccccagct ggatctgact atcaacaatg gcgagttcct cacgctgctt ggcccttctg
    10981 gctgcggtaa aacaaccgtt cttcgcctga ttgcaggtct ggaaactgtt gattccggac
    11041 gcatcatgct ggataacgag gacatcaccc acgttccggc ggaaaaccgc tatgtgaaca
    11101 ctgttttcca aagctacgca cttttccccc acatgaccgt gttcgaaaat gtggcctttg
    11161 ggttgcgcat gcaaaaaacc cccgctgctg aaattacgcc ccgcgtgatg gaagccctgc
    11221 ggatggtgca actggaaacc ttcgctcaac gtaaaccgca tcagctctct ggtggtcaac
    11281 agcaacgcgt cgccattgct cgcgcggtgg ttaacaagcc tcgtctgttg ttgctggatg
    11341 agtcgctctc agcgctggat tacaaactgc gtaagcaaat gcagaacgag ctgaaagcgt
    11401 tacagcgtaa gcttggtatt accttcgtct ttgtcactca cgatcaggaa gaagcgctca
    11461 ccatgtcaga caggattgtg gtgatgcgcg agggtcgtat tgagcaagat ggtacaccgc
    11521 gcgaaattta cgaagagccg aaaaacctgt ttgttgccgg cttcattggc gaaatcaata
    11581 tgtttaacgc cactgtcatc gaacgtctag acgagcagcg cgtacgcgcc aacgttgaag
    11641 gccgcgaatg taatatctac gttaacttcg ccgttgaacc ggggcaaaaa ctgcatgttc
    11701 tgctgcgccc ggaagactta cgtgttgaag agattaacga cgacaaccac gctgaagggc
    11761 tgattggtta cgttcgtgag cgtaactaca aaggcatgac gctggagtcg gttgttgaac
    11821 tggaaaatgg caagatggtg atggtcagcg aattcttcaa tgaagacgac cctgactttg
    11881 accactctct cgaccaaaaa atggccatta attgggtaga aagctgggag gtcgtactgg
    11941 ctgatgaaga acacaagtaa gttccagaat gtagtgattg tcactattgt cggttggctt
    12001 gtgttgtttg tctttctgcc caacctgatg atcattggca ccagcttttt gacccgcgac
    12061 gacgccagtt tcgtcaaaat ggtctttacg ctggataact acacgcgtct gctcgatccg
    12121 ctctattttg aagtgctatt gcactcgctg aatatggcgc tgatcgccac cctcgcctgc
    12181 ctggtgctgg gctacccgtt tgcctggttt ctggcgaagc taccacacaa ggtgcgtccg
    12241 ctgctgctgt ttctgctgat tgttcccttc tggaccaact cactgattcg catctacggg
    12301 ctgaaaattt tcctcagcac caaaggctat ctcaacgagt ttttgctctg gctgggcgtt
    12361 atcgacacac caattcgtat catgttcacg cccagtgcgg tgattatcgg tctggtttac
    12421 attctgctac cgtttatggt gatgccgctg tactccagta tcgaaaaact ggataagccg
    12481 ttgcttgagg cggcgcgcga tctcggtgcc agcaagttac agacttttat ccgtatcatt
    12541 attccactga cgatgccggg aattattgcc ggatgtctgc tggtgatgct gccagcgatg
    12601 ggcctgttct atgtatccga cctgatgggc ggtgcgaaaa acctgctgat cggtaacgtc
    12661 atcaaggtcc agttcctcaa tattcgtgac tggccgtttg gtgcagccac cagcattacg
    12721 ctgactatcg taatgggcct gatgttgctg gtttactggc gcgcttctcg tctgctgaat
    12781 aaaaaggtgg aactcgaatg atcggtcgac tgcttcgcgg cggttttatg accgctatct
    12841 acgcgtacct gtatatccca atcattattt tgattgtgaa ctcctttaac agttcgcgct
    12901 ttggcatcaa ctggcagggt tttaccacca aatggtatag cctgctgatg aacaacgaca
    12961 gcctgttaca ggcagcgcag cattcactaa caatggcggt gttttcggcg acatttgcta
    13021 cgcttatcgg ttcactgacg gcagttgcgc tgtaccgtta tcgctttcgt ggtaagccgt
    13081 tcgttagcgg aatgctgttt gtggtgatga tgtcaccaga tatcgtgatg gcgatttctc
    13141 tgctggtgct gtttatgctg ctgggtattc agcttggctt ctggtcgctg ctgttctcgc
    13201 atatcacctt ctgcctgccg tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg
    13261 acgtgcggat gctggaagcg gcgaaagatc tcggtgccag tgaatttacc attctgcgaa
    13321 aaatcattct gccactggca atgcctgcgg tggcggcggg ctgggtgtta agctttaccc
    13381 tgtcgatgga cgatgtggta gtttcttcgt tcgtcaccgg accaagttat gaaattctgc
    13441 cgttaaaaat ttattcgatg gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa
    13501 ccatattact ggtgctgtcg ctggtgatgg ttattgccag ccagcttatt gctcgtgata
    13561 aaacgaaagg taacacaggg gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc
    13621 gggtgctctg gcactgggca tgagcgccgc tcacgccgat gacaacaaca cgctgtattt
    13681 ctacaactgg accgagtacg tgccgccagg actgcttgaa cagttcacca aagaaaccgg
    13741 tattaaggtt atctattcga cttacgagtc gaacgaaacc atgtatgcca agctgaaaac
    13801 gtacaaagac ggtgcctatg acctggtggt tccttcaacc tattacgtcg ataaaatgcg
    13861 taaagaaggg atgatccaga agatcgacaa gtcgaagtta acgaacttca gcaatctcga
    13921 tccagacatg ctcaataagc cgtttgaccc gaataacgat tactccattc cgtatatctg
    13981 gggtgcgacg gcgattggtg ttaacggtga tgcggtggat ccgaaatctg tcaccagctg
    14041 ggccgatctg tggaagcctg agtacaaagg cagcctgctg ttgaccgacg atgcccgtga
    14101 agtgttccag atggcgctgc gtaagctggg ctactccggt aacaccaccg atccgaaaga
    14161 gattgaagct gcatataacg agctgaaaaa actgatgcca aacgtggcag cctttaactc
    14221 cgataacccg gctaacccgt acatggaagg cgaagttaac ctcggcatga tctggaacgg
    14281 ttctgctttc gttgcacgcc aggcgggtac gccaattgac gtggtatggc cgaaagaagg
    14341 cggcattttc tggatggaca gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc
    14401 gctgaaattg atcaacttcc tgctgcgccc ggatgtggca aaacaggttg ccgaaactat
    14461 cggttatcca acgccaaacc ttgcggcgcg taagctgtta agcccggaag tggcgaacga
    14521 taaaacgctc tacccggatg ctgaaaccat taaaaatggt gaatggcaga atgacgttgg
    14581 cgcagccagc agcatttatg aagagtatta tcagaagctg aaagcaggac gttaattcag
    14641 caagcgggcg gtggcattac cgcccgtttt ttatttagca aggatgtggc taatgagtca
    14701 ggacagtaaa gtgttatccc gcgcttttct cggtatcggt ctgatattgc tcgttatttc
    14761 tgcaatcatc ttttattacc attttacttt taccaaaagc gcggttcata cagaaggaat
    14821 cattgttgat gccgtttggt acaacaatca ctcaaacgac gtcgatgata atggttcctg
    14881 gtatccagtg gttgcctttc gccctacgcc agattacacg ctaattttca attcgaatat
    14941 cggtagtgat ttttatgaag atagcgaagg tgacagagtc aatgtctatt atccgccagg
    15001 gcatccagaa caagccgaaa taaacaatcc ctgggttaat ttttttaaat ggggctttgt
    15061 tggcatagca ggcattattt tcggtagtgt cggtctgata atcacgctcc cctccaccaa
    15121 aaaatctcgc aggaaacgga agtcccgccc atgaaaaaat tcattatact gctcagttta
    15181 ctgatactcc tgcctctggt agcaaccagt aaaccattga ttccaataat gaagacactt
    15241 tttacggatg ttaccggtac tgtaccggat gctgaagaga tcgcacgtaa agccgagctt
    15301 tttcgccaac aaacgggaat tgcaccgttt attgtgattt tacccgacat taataatgaa
    15361 gccagtctca gacaaaatgg taaagcgatg ctggcgcatg cgtcatcttc attgagtaat
    15421 gtaaaaggaa gtgttctgtt actatttact acccgcgaac cacggttaat tatgatcacc
    15481 aacggccagg ttgaaagtgg tctggacgat aaacatctcg gccttctgat agaaaatcac
    15541 acgctggctt atttaaatgc agatctctgg tatcagggaa tcaataatgc attggctgtt
    15601 ctacaagcac agatattaaa acaaccgacg ccgccactga cgtattatcc gcatccagga
    15661 caacagcatg aaaatgctcc ccccggcagc accaacacat taggttttat cgcctgggct
    15721 gcgacgttta tcttattttc ctggatcttt tgctacacga ctcgttttat ttatgcgtta
    15781 aaatttgcag tggcgatgac tattgccaat atgggttatc aggctttatg tttgtatatc
    15841 gataatagct ttgcgattac cagaatatcg ccactctggg cagggctaat tggtgtctgt
    15901 acgtttattg ccgcattgtt attgacgagt aaacgataaa agaggccgga aaagtgcgcg
    15961 tgtattatgc cggatggggc gcgaacgcct tatccggccc acaaaacccg caaattcaat
    16021 taattgcgtc cccttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta
    16081 taatcccttc agcaactttt caacaaactc aggcaccacc tggcttgccg ggccgtaata
    16141 tttctcggca aattcattac cgacctgact tggttcgaga ttcagttcca cggtgtgcgc
    16201 gccatgcagt ttcgcttcgt gaacaaaccc agccgccgga taaacatgcc cggaagtacc
    16261 aatggcaatg aaaatatcag ccatcgacaa cgccatataa atttcatcca tgccgagtgg
    16321 catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca
    16381 tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg
    16441 aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag
    16501 gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agggcgtctt gcagtttagc
    16561 cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc
    16621 gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc
    16681 ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc
    16741 cgcagaaatc cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac
    16801 cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt
    16861 acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc
    16921 ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc
    16981 aatgcgcgga acacgagcta caggtaagag atgacgaggc agcctgtccg ccagttgcgt
    17041 tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat
    17101 ggtcaggata tttcccagac aaaccgctaa taaatccaga taacgctcaa cgtgcgccct
    17161 tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg
    17221 ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc
    17281 atgctgacca cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc
    17341 aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttaccgg taatcggttt
    17401 gccgttgaaa atcagcccgc cgccaacgcc ggtgccgaga atcaacccca tcaccagtgg
    17461 atattgagta aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc
    17521 aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt taccgctggc
    17581 agcggggaca ttggcggcat acagcgtacc atcttctgtt tccggcattc ccggaatacc
    17641 gatgccgaca gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac
    17701 tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa
    17761 ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta
    17821 atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag
    17881 ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg
    17941 ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg aaatcaataa
    18001 aatagatatc gctggagagg aactgatgac cgatcaactt ttcaatccac tcaataatcg
    18061 gggtaagttg cagtgaaacc accacaccga taatcacccc acacaggctg ccgaacagcc
    18121 ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg
    18181 ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga
    18241 tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca
    18301 tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc
    18361 tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt
    18421 taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg
    18481 ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg gcaatgtgca
    18541 aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg atcatgatcg
    18601 acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg atgataattt
    18661 gctgttcgcc cgctttaaaa ttgcgccagg catccccctg aacaaacgag ggtaatgcgc
    18721 tcagacgctg ttcctgttgc gggttaacgc ccttcacctg aattgcgcgc agattggcac
    18781 cactttctac cagtccggtg aaattgatat acggcgcagc agcggcaata cccggcactt
    18841 tttgcacgtt atccagtgct tcctgccagt tagtccatgg ctgatttacc gcctcaattt
    18901 caccatgcgg caccaccgcc agaatacggt tattcagttc gcgttcaaag ccgttcatcg
    18961 cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta gaaataacgg
    19021 agatcagcga caccatgccg ccgcgccgcc gtccgcgact aaaacgcagg ccaatcaata
    19081 acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg tcagacgtcc
    19141 atcacgcatt tccaattggc ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac
    19201 caggaaggcg gtgccctgca agcgattcaa ttccccaagc aactggaaga tgctgtcggc
    19261 gttacgcgca tcgaggttac cggtaggctc atccgcaagt accagacgcg gattattgac
    19321 cagcgcacgg gcaatcgcca cacgctggcg ttcgccgcca gaaagttcag atgggcggtg
    19381 attcgcacga tgctccagcc ccaccgcttt taacatctcc agcgcacggc tgttgatttc
    19441 agcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg cagtaaaatc
    19501 cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca gttctgcttt
    19561 cgccgccgaa gacagtttgc tcatcggttg accgttaaag atcacatcgc cggaggttgg
    19621 tgtatccagc ccgcccagca ggtgcagcaa ggtactttta ccggaaccag agctaccgac
    19681 gatcgccatc atttcacctt cgcccacgct gaaactgaca ttgtgcagta catcggtttg
    19741 cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat
    19801 aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca
    19861 gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcca
    19921 ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc
    19981 tggcaagcag tgcgccaagc gccgcaccga ggatcgcacc gataatcccg gcgctggccc
    20041 cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg
    20101 cgacttcgcc ctgcttctcc attaccatca gccctagtga ggtaataatg ttaaacgccg
    20161 caacggcgac aatcaggctc agcagcaagc ccatcatatt tttttccatg cgtacggcct
    20221 ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca ggcagttttt
    20281 gctgacttaa agagtcaact ttcagcggct catccagcca caaacgccag ccggtaatat
    20341 tgcctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc atttcatagc
    20401 catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tggcttggaa
    20461 tacgccccat cggcgtgaac tggctggcag atggtaccat cacgcggatt tgatcaccgc
    20521 gattaacgcc tagctgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct
    20581 cgaggtcagt ttgtttcaca ttgaccagat acggtgtaag tggatctttt tgcgccgggt
    20641 cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg
    20701 tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct
    20761 gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt
    20821 tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca
    20881 ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg
    20941 cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg
    21001 tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga
    21061 actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc
    21121 catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca
    21181 gatacccatt acacctcggg tcataacccg caggatcaac cgcatgtaaa ttaaaaaaga
    21241 gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat
    21301 ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatctga ccacttttca
    21361 gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc
    21421 ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct atatcgacag
    21481 cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct
    21541 ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg
    21601 cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg
    21661 gatgatcacc gccagcacca gcttgtggta tctgtatgcg ttaattgtct atttcgtggt
    21721 atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt
    21781 ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatcc gcaatttgct
    21841 ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc
    21901 gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt
    21961 gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta
    22021 tcaatacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa
    22081 caccattgct atctacacca cccatcgcat tctggttgaa atattcagct taactctgct
    22141 tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta
    22201 tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca
    22261 gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc
    22321 tcgttaaacc atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat
    22381 aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga
    22441 atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc
    22501 agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg
    22561 aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc
    22621 atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc
    22681 ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt
    22741 accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc
    22801 gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc
    22861 tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg
    22921 tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga
    22981 gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg
    23041 acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcgcacg
    23101 aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg
    23161 aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg
    23221 ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt
    23281 tccctgccaa taccttgctg gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc
    23341 aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc
    23401 caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc
    23461 gggtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc
    23521 agaaactgcc agacctggcc gttcaggcac aacaaaaagc gccgctggat gcgctgcgta
    23581 agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg
    23641 aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg
    23701 atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg
    23761 ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc
    23821 gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg
    23881 agctgcatat tggtcagccg gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa
    23941 tgaccacgct cgaagcgggc ggcattactg gcgagtattt gatgctcacc tatgccaacg
    24001 acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg
    24061 cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga
    24121 aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg
    24181 ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca
    24241 gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca
    24301 tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag
    24361 aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggtg gcggtgttgg
    24421 tgcctaccac ccttctcgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact
    24481 ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc
    24541 ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa
    24601 gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg
    24661 tgcgtcataa agagcgtatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg
    24721 caacgccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta
    24781 tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc
    24841 tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct
    24901 acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg gtgccagaag
    24961 cgcggatcgc catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg
    25021 atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg
    25081 acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc
    25141 tgcaccagtt acgcggtcgc gtcggacgtt cgcatcatca ggcatatgca tggttgctga
    25201 cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc
    25261 tggaagatct cggtgcaggc tttgcgctgg caacgcacga tctggagatt cgcggcgcgg
    25321 gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata
    25381 tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag
    25441 atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt
    25501 tcattcctga cgtgaatacg cgtttgtcgt tctataaacg tattgccagc gccaaaacgg
    25561 aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc
    25621 cggcgcgtat cctgctggat gttgcccgtc tgcgccagca agcgcagaaa ctggggatca
    25681 ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta
    25741 atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc
    25801 cgacgcgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac
    25861 gccagtttat gcgtgaactg gaagagaacg cgatcgctta atggtaaatc gggggcgttt
    25921 ctgcgccccc atctgcaaca tttacaaatt ctttgcactt ccctgcacta tccgacactg
    25981 tcaccatcca taattcaggc ttatctgttt attacaataa ccttatattt attatggatt
    26041 tttggtgatg atcaaaacgc gtttttctcg ctggctaacg ttttttacgt tcgccgctgc
    26101 cgtggcgctg gcgctaccgg caaaagccaa cacctggccg ctgccgccag cgggcagtcg
    26161 tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac ggtggttctc tggaagccat
    26221 cgccaaaaaa tataacgtcg gctttctcgc tctgttacag gctaaccccg gcgttgatcc
    26281 ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg caaaccctac ttccagacgc
    26341 gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt ctctattact acccgccggg
    26401 taaaaattcg gtaaccgtgt atccaatagg tattggtcag ttaggtggtg acacgctgac
    26461 accgacaatg gtgaccaccg tttcagacaa acgtgcaaac ccaacctgga cgccaacggc
    26521 aaacatccgc gcccgttata aagcacaggg aattgagttg cctgcggtag tgccggctgg
    26581 accggataac ccaatgggcc atcatgcgat tcgtctggcg gcctatggcg gcgtttattt
    26641 gcttcacggt acgaacgccg atttcggcat tggcatgcgg gtaagttctg gctgtattcg
    26701 tctgcgggat gacgatatca aaacactctt tagccaggtc accccaggca ccaaagtgaa
    26761 tatcatcaac actccgataa aagtctctgc cgaaccaaac ggtgcgcgtc tggttgaagt
    26821 acatcagccg ctgtcagaga agattgatga cgatccgcag ctgctgccaa ttacgctgaa
    26881 tagcgcaatg caatcattta aagatgcagc acaaactgac gcagaagtga tgcaacatgt
    26941 gatggatgtc cgttccggga tgccggtgga tgtccgccgt catcaagtga gcccacaaac
    27001 tctgtaaatc ctggataaaa aaaatccccg cgacacggca tgtcgcgggg aaaaaatcag
    27061 cagtagcagg cattaatgag ggttaatgct tatttataaa ttactgctgt tccatggagg
    27121 gtattcggac cggttacaga agtaatacgg aaagattttg cgcccatctc atccgctttt
    27181 tgcgccagct gctcttccag cgatcccaga tttgtccccg cgttagcact gattgtaccg
    27241 actttttgtt ggccttctgg cgttgactga acttcgacag ccgcaaagct ggcaaatgac
    27301 atggagctta aaatcgccgc agcgatgagg gtttttacgt ttttcataat agtggcctta
    27361 tgcagatgaa tgacgtcgca agcaattact taacgatcga taactaaatg a
//
LOCUS       DAPHJE010000059        27320 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPHJE010000059 DAPHJE010000000
VERSION     DAPHJE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.059"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            227..2650
                     /gene="pgaA"
                     /locus_tag="RH222_002514"
     CDS             227..2650
                     /gene="pgaA"
                     /locus_tag="RH222_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV3797516.1"
                     /translation="MYSSSRKRCPKTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSID
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2659..4677
                     /gene="pgaB"
                     /locus_tag="RH222_002515"
     CDS             2659..4677
                     /gene="pgaB"
                     /locus_tag="RH222_002515"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDV3797517.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANSSQLDSIPRVLIANNPSLKEFAQQIITVQEKSSQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDKVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHDQPEIDVIRPEFSTAWYPKND"
     gene            4670..5995
                     /gene="pgaC"
                     /locus_tag="RH222_002516"
     CDS             4670..5995
                     /gene="pgaC"
                     /locus_tag="RH222_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV3797518.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDRTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5997..6410
                     /gene="pgaD"
                     /locus_tag="RH222_002517"
     CDS             5997..6410
                     /gene="pgaD"
                     /locus_tag="RH222_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDV3797519.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6460..7248)
                     /gene="phoH"
                     /locus_tag="RH222_002518"
     CDS             complement(6460..7248)
                     /gene="phoH"
                     /locus_tag="RH222_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV3797520.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7867..9138)
                     /gene="efeB"
                     /locus_tag="RH222_002519"
     CDS             complement(7867..9138)
                     /gene="efeB"
                     /locus_tag="RH222_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV3797521.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9144..10271)
                     /gene="efeO"
                     /locus_tag="RH222_002520"
     CDS             complement(9144..10271)
                     /gene="efeO"
                     /locus_tag="RH222_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV3797522.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10329..11159)
                     /locus_tag="RH222_002521"
     CDS             complement(10329..11159)
                     /locus_tag="RH222_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV3797523.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(11702..13210)
                     /gene="putP"
                     /locus_tag="RH222_002522"
     CDS             complement(11702..13210)
                     /gene="putP"
                     /locus_tag="RH222_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV3797524.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            13632..17594
                     /gene="putA"
                     /locus_tag="RH222_002523"
     CDS             13632..17594
                     /gene="putA"
                     /locus_tag="RH222_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3797525.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARSESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17634..18272)
                     /gene="rutR"
                     /locus_tag="RH222_002524"
     CDS             complement(17634..18272)
                     /gene="rutR"
                     /locus_tag="RH222_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV3797526.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            18560..19651
                     /gene="rutA"
                     /locus_tag="RH222_002525"
     CDS             18560..19651
                     /gene="rutA"
                     /locus_tag="RH222_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV3797527.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19651..20343
                     /gene="rutB"
                     /locus_tag="RH222_002526"
     CDS             19651..20343
                     /gene="rutB"
                     /locus_tag="RH222_002526"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV3797528.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20355..20741
                     /gene="rutC"
                     /locus_tag="RH222_002527"
     CDS             20355..20741
                     /gene="rutC"
                     /locus_tag="RH222_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV3797529.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            20749..21549
                     /gene="rutD"
                     /locus_tag="RH222_002528"
     CDS             20749..21549
                     /gene="rutD"
                     /locus_tag="RH222_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV3797530.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARVPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21559..22149
                     /locus_tag="RH222_002529"
     CDS             21559..22149
                     /locus_tag="RH222_002529"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV3797531.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22160..22654
                     /gene="rutF"
                     /locus_tag="RH222_002530"
     CDS             22160..22654
                     /gene="rutF"
                     /locus_tag="RH222_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV3797532.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            22675..24003
                     /gene="rutG"
                     /locus_tag="RH222_002531"
     CDS             22675..24003
                     /gene="rutG"
                     /locus_tag="RH222_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV3797533.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24086..24259)
                     /locus_tag="RH222_002532"
     CDS             complement(24086..24259)
                     /locus_tag="RH222_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV3797534.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24632..25228
                     /gene="wrbA"
                     /locus_tag="RH222_002533"
     CDS             24632..25228
                     /gene="wrbA"
                     /locus_tag="RH222_002533"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV3797535.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25249..25476
                     /locus_tag="RH222_002534"
     CDS             25249..25476
                     /locus_tag="RH222_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV3797536.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25514..26755)
                     /gene="agp"
                     /locus_tag="RH222_002535"
     CDS             complement(25514..26755)
                     /gene="agp"
                     /locus_tag="RH222_002535"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV3797537.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27047..>27320)
                     /locus_tag="RH222_002536"
     CDS             complement(27047..>27320)
                     /locus_tag="RH222_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797538.1"
                     /translation="PEQKVNEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDDD
                     LEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAIY"
BASE COUNT         6683 a         7051 c         6932 g         6654 t
ORIGIN      
        1 ggatttatgc cgtattcctg aagatcctca tcattggaat ggattttcgg gcgagaaaag
       61 gattttatat ggacactctg ctcatcattt cttcttctca tcatcaacaa ttcacgtctc
      121 tcttccgcgt ttaataacgg attatgaggt gcaaaaatat ctttcttttc agttacctgt
      181 aattagatac agagagagat tttggcgata catggagtaa tacaggatgt attcaagtag
      241 cagaaaaagg tgcccgaaaa ccaaatgggc tttgaaaatt cttactgccg catttttagc
      301 agcgagtccc gcggcgaaga gtgccgttaa taacgcctat gatgcattga ttattgaagc
      361 tcgcaaggga aatactcagc cagctttgtt atggtttgca caaaaatcag cactcagtaa
      421 taaccaaatt gctgactggt tacagattgc attatgggcc gggcaagata aacaggttat
      481 taccgtttac aaccgctacc gttatcagca attaccagcg cgtggttatg cagctgtcgc
      541 cgtcgcttat cgtaacctgc aacaatggca aaactcgctt acactgtggc aaaaggcgct
      601 ctctctggag ccgcaaaata aggattatca acggggacaa attttaaccc tggcagatgc
      661 gggtcactat gatactgcgc tggttaaact taagcagctt aactctggag caccggacaa
      721 agccaattta ctcgcagaag cctatatcta taaactggcg gggcgtcatc aggatgaatt
      781 acgggcgatg acagagtcat tacctgaaaa tgcatctacg caacaatatc ccacagaata
      841 cgtgcaggca ttacgtaata atcaacttgc tgccgcgatt gacgatgcca atttaacgcc
      901 agatattcgc gctgatattc atgccgaact ggtcagactg tcgtttatgc ctacgcgcag
      961 tgaaagtgaa cgttatgcca ttgccgatcg cgccctcgcc caatacgctg cattagaaat
     1021 tctgtggcac gataacccag accgcactgc ccagtaccag cgtattcagg ttgatcatct
     1081 tggcgcgtta ttaactcgcg atcgttataa agacgttatt tctcactatc agcgattaaa
     1141 aaagacgggg caaattattc cgccctgggg gcaatattgg gttgcatcgg cttatctcaa
     1201 agatcatcag ccgaaaaaag cacagtcaat aatgaccgag ctcttttatc acaaggagac
     1261 cattgccccg gatttatccg atgaagaact tgcggatctc ttttacagcc acctggagag
     1321 tgaaaattat ccgggcgcgc taactgtcac ccaacatacc attaatactt cgccgccttt
     1381 ccttcggtta atgggcacgc ctacgagcat cccgaatgat acctggttac aggggcattc
     1441 gtttctctca accgtagcaa aatatagtaa tgatcttcct caggctgaaa tgacagccag
     1501 agagcttgct tataacgcac caggaaatca gggactgcgc attgattacg cgagtgtgtt
     1561 acaagcccgc ggttggcctc gtgcagcaga aaatgaatta aaaaaagcag aagtgatcga
     1621 gccacgtaat attaatctgg aggttgaaca agcctggaca gcattaacgt tacaagaatg
     1681 gcagcaggca gctgtcttaa cgcacgatgt tgtcgaacgt gaaccgcaag atcccggcgt
     1741 tgtacgatta aaacgtgcgg ttgatgtaca taatcttgca gagcttcgta tcgctggctc
     1801 aacaggaatt gatgccgaag gcccggatag tggtaaacat gatgtcgact taaccaccat
     1861 cgtttattca ccaccgctga aggataactg gcgcggtttt gctggattcg gttatgccga
     1921 tggacaattt agcgaaggaa aagggattgt tcgcgactgg ctggcgggtg ttgagtggcg
     1981 gtcacgtaat atctggctcg aggcggagta cgctgaacgc tttttcaatc atgagcataa
     2041 acccggcgcg cgcctgtctg gctggtatga ttttaatgat aactggcgta ttggttcgca
     2101 actggaacgc ctctctcacc gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa
     2161 cagtgctcag gcttatgttc gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg
     2221 ggctttcact gatttttccg acagtaacca gcgtcatgaa gtctcaattg atggtcagga
     2281 acgcatctgg tcttcaccat atttgattgt cgatttccta cccagtctgt attacgaaca
     2341 aaatacagaa cacgataccc catactacaa ccctataaaa acgttcgata ttgttccggc
     2401 atttgaggca agtcatttgt tatggcgaag ctatgaaaat agctgggagc aaatcttcag
     2461 cgcaggtgtt ggtgcctcct ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg
     2521 ctacgggcaa cgcattagct ggaatgacgt gattgatgct ggcgcaacgc tacgctggga
     2581 aaaacgacct tatgacggtg acagagaaca caacttatac gttgaattcg atatgacatt
     2641 cagattttaa ggataaatat gttacgtaat ggaaataaat atctcctgat gctggtgagt
     2701 ataattatgc tcactgcgtg cattagccag tcaaaaacat catttatacc gccacaggat
     2761 cgcgagtctt tactcgccga gcaaccgtgg ccgcataatg gttttgtggc gatttcatgg
     2821 cataacgttg aagacgaagc tgccgaccag cgttttatgt cagtgcggac atcagcactg
     2881 cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac ctgtcagtat tgctcaaatt
     2941 cgtgaagcac atcgaggagg aaaaccgcta ccggaaaaag cagtagtgct gacttttgat
     3001 gacggctacc agagttttta tacccgcgtc ttcccaattc ttcaggcctt ccaatggcct
     3061 gctgtatggg cccccgtcgg cagttgggtc gatacgccag cggatgaaca agtaaaattt
     3121 ggcgatgaga tggtcgatcg agaatatttt gccacgtggc aacaagtgcg agaagttgcg
     3181 cgttccccgc tcgttgaggt cgcttctcat acatggaatt ctcactacgg tattcaggct
     3241 aatgccaccg gcagcttatt gcctgtatat gtaaatcgtg catattttac tgaccacgca
     3301 cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc tggatgctgt aaaaatgaca
     3361 gaatacctgc gtacaaaggc taaggtaaat ccacacgttt ttgtttggcc ttatggcgaa
     3421 gcgaatggca tagcgataga ggaattaaaa aaactcggtt atgacatgtt cttcaccctt
     3481 gaatcaggtt tggcaaattc gtcacaattg gattccattc cgcgagtatt aatcgccaat
     3541 aatccctcat taaaagagtt tgcccagcaa attattaccg tacaggaaaa atcatcacaa
     3601 cggataatgc atatcgatct tgattacgtt tatgacgaaa atcgccagca aatggatcgc
     3661 aatattgatg tgctaattca gcgggtgaaa gatatgcaaa tatcaaccgt gtatttgcag
     3721 gcatttgctg atcccgatgg tgatgggctg gtcaaagagg tctggtttcc aaatcgtttg
     3781 ctaccaatga aagcagatat ttttagtcgg gttgcctggc aattacgtac ccgctcaggt
     3841 gtaaacatct atgcgtggat gccggtatta agctgggatt tagatcccac attaacgcga
     3901 gtaaaatatt taccaacagg ggagaaaaaa gcacaaattc atccagaaca atatcgccgt
     3961 ctctctcctt ttgatgacaa ggtcagagca caagttggca tgttatatga agatcttgcc
     4021 ggacatgctg cttttgatgg cctattgttc cacgatgatg ctttgctttc agattatgaa
     4081 gatgccagtg caccggctat cacggcctat cagcaagcag gctttagcgg gagtctgagc
     4141 gaaattcgac aaaacccgga gcaatttaaa cagtggaccc gctttaaaag tcgtgcgtta
     4201 actgacttca ctttagaact tagtgcgcgc gtaaaagcca ttcgcggtcc acatgttaaa
     4261 actgcacgaa atatttttgc acttccggta atacaacctg aaagtgaagc ctggtttgca
     4321 cagaattatg ctgatttcct aaaaagctat gactggaccg ctattatggc tatgccttat
     4381 atggaaggtg tcgcagaaaa atcggctgac caatggttaa tacaattgac caatcaaatt
     4441 aaaaacatcc ctcaggctaa agacaaatct attttagaat tacaggcaca aaactggcag
     4501 aaaaatggtc agcatcaggc tatttcttcg caacaactcg ctcactggat gagcctatta
     4561 caactgaatg gagtgaaaaa ctatggttat tatcccgaca attttctgca tgaccaacct
     4621 gaaatagacg ttattcgtcc tgagttttca acagcctggt atccgaaaaa tgattaatcg
     4681 catcgtatcg ttctttatat tatgtctggt gctatgcatc cccctatgcg tagcgtactt
     4741 tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta tgtccattat
     4801 gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc cgtggggaga
     4861 aaacgcacca gctccccagt tgaaagataa tccgtctatc tccattatca ttccctgttt
     4921 taatgaggag aaaaacgtag aggaaaccat acacgccgct ttagcacagc gttatgagaa
     4981 cattgaagtt attgccgtaa atgacggttc aacagataga acccgtgcca tcctggatcg
     5041 catggctgca caaattcccc atttgcgggt cattcatctg gcgcaaaacc aggggaaagc
     5101 cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt gcattgatgg
     5161 cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt tgtacaaccc
     5221 gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc tggtgggtaa
     5281 aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc agcgtatcta
     5341 tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg ccctggcaga
     5401 agtgggttac tggagtgacg atatgatcac cgaagatatt gatattagct ggaagctgca
     5461 gttgaatcag tggacgattt tttacgagcc acgggcgctg tgctggatat taatgcctga
     5521 aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg cagaagtatt
     5581 cctcaaaaat atgacaaggc tatggcgcaa agaaaacttt cgaatgtggc cactgttttt
     5641 tgaatactgt ctgacgacaa tatgggcctt tacctgcctg gtcggtttca ttatttacgc
     5701 agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg cgacacatac
     5761 tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc tgatgatcga
     5821 gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt tcccggttat
     5881 tttctggatg ctgagcctgg caacgacatt ggtatcattt acacgagtca tgttgatgcc
     5941 taaaaagcaa cgcgcccgtt gggtaagtcc cgatcgcggg attctgagag gttaatatga
     6001 acaatttaat tattacgacc cgacaatcgc cagtacgttt actggttgat tatgttgcca
     6061 caaccatctt gtggacatta tttgctttgt tcatattctt attcgccatg gatctgctga
     6121 cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt ttgctggcag
     6181 tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctgcgt tttcaaaaac
     6241 agcagcatca tgcagcctac caatatacgc cacaagaata tgcagagagc ttagcaatac
     6301 ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac ttcaccagcc
     6361 aggggcaaat aaaaatggtt gtttcagaaa aagcgctagt ccgggcataa acacccaaaa
     6421 caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg catgcagcgt
     6481 acgttggcaa agtgccgaac gtacgcaatc ctctttaccg aatcggatga tcccgaccat
     6541 ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcgcacac cgcgaggtaa
     6601 atcgcattgt gtgatatcac cgttgacgat aaccgtcacg ttctccccga ggcgggttaa
     6661 aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga cgactgcatt
     6721 ttcaaaggta cgtccacgca tataggcgaa cggcgcgatt tccaccttcc ctatttccgg
     6781 tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcacgt cgtagaccgg
     6841 gcgaaaatag ggagcaaact tttctgcgat atctccaggt aagaagccaa gatcttcatc
     6901 ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta tcagggcctc
     6961 tgccgctttt gctgcactga tccaggtttt tccgcacccg gcttcgcccg tggcgaatat
     7021 cagctgctta ctctcaatag ccttcagata gtgcaattgc gcttcatttc gcgcgaggat
     7081 gggcgaggta tcgcgactgt cgcgagccat accaatggct tctacgccgc tcatctgcac
     7141 aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc gtctcagcac
     7201 acgttttgcc tcacgacgag ctttgatcac tgctttttgt cttcccatgg agagcacctt
     7261 gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac gaacatcaga
     7321 gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa aacggctacg
     7381 cacaccgttt gcggcgtcgg taacacgagt aaagagagga agtgagaaaa atacagagtg
     7441 acgaagttgc taccggagga gaaatctccg cgagtggtgg agcgttgctt aacaaaacat
     7501 tgtccagtac aggacgttac catccgcgat ctccatagtg actgactatc actgccggga
     7561 acttcctctg ctacttaata agtacaacag atctcgcatt cattgcaaca atatattcac
     7621 ttatatttaa ctataaaaca ccattttagt gacattagtt tctactggaa agatgacaga
     7681 gtgatgacag agatgaaaaa agctgtgtgc tttcagcagg atttgcatca ggcatctgtg
     7741 cacgatcgcc gtatgcggcg tgaacgcctt atccgcctac cgaacccgca ccgattgtag
     7801 gcataagaag acgcgcaagc atcgcatcag gcatctgcgc aagaacgcct tatccgtcta
     7861 aaaacattaa acccgcaata acgcgcttcc gagataatcg ttcgcgtcct tcactcccgg
     7921 cagcgcaaaa aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt
     7981 gagccttttt tgtactgtca ggaagccttt ttccagatcg tgttggtaac agacaaacag
     8041 tagccccata tccagttgcc cggagttggt gacgcccagt gaataactgt agccacgacg
     8101 cagcatcagg ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc
     8161 cagcgcaatc accttccctt ccggatcgct ggcgtaatcg ggaacatcat gctcatgctg
     8221 cattcccagc ggcgcaccgg tttgcttatc acgaccaaaa atcgtctgct gttctttcag
     8281 cggagttctg tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc
     8341 accgattgtc cacgctggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa
     8401 cttatcattc tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg
     8461 cgtctctttg cctttactac gcgccgcgtg atcggaaata aacccttccc gcttccagcg
     8521 cacgctgagc aaatctggcg tatgtttgat gatatcgcgc agcgcatgga tcaccgtgtc
     8581 ctgggtgttg gcgcaaattt gtagcaacac atcaccatga cataacgccg catccagcga
     8641 gtcgttggga aaacgcgtca tcttctgcaa ctttttcggc atctgtggcg caaggccaaa
     8701 gcgctcatca aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat
     8761 gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc ggatttggcg tttctggtgc
     8821 cgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac aagcgaaaca accgttcaag
     8881 atcgggttta tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg
     8941 cgtcaggatc cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg
     9001 tgaaagcgta cccggcgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc
     9061 cagcgccagc gcaccaatgc ctttcagtaa acgtcggcgt gacggttcat tcacgccgtt
     9121 tttatcttca tactgcataa tgcttaatcc aatcccagca caccgcgaag ttgcgccaga
     9181 tcttccgcca gcgcagtaat cggtcctttc agtgcattcc ggtcggcatc ggtcaatttg
     9241 tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca gaatggtatc gaccttttta
     9301 aagttggcat cgacttttgc caacagttcc gggttggctt tttgcagttg tggacgcagc
     9361 agatcgacaa ttttctgcga gccttcaacg ttagcctgga aatcccacag atcggtgtgg
     9421 ctgtagcgat cttcttcacc gctgattttg ctggctgcca cttcctcaat cagtccggct
     9481 gcaccgccga ccacttttga aggtgggaaa gccagttcac tgatgcgttt ttgcaaatcg
     9541 accacatcgg tataaagctg gtcagcgtac tgatccatgc ctttggtggt gttgtcgcca
     9601 aacaatgctt tttccagacg gtggaaaccg gtgaatttcg gatcggcggc tttttgctcg
     9661 taatcatctt cacgggcgtc aatgctgcca tccagatcgg agaacagttc agcaatcggt
     9721 tcgatgcgct catagtgctg gcgcgtcggc gcatacagtg ctttcgcttt ttcgatatcg
     9781 cctgctttaa tagcgtcagt aaaggctttc gtgtcggtca ccagctgcgt ggtttccgcc
     9841 atgacatacg ctttatatgc agtaattgca ccaccaagac ttaacagcgc atcactttgc
     9901 gccgcatccg ccgttgcctc acctttgacg atcaacttcc ctttcgggtt agtcagcaga
     9961 ccgcaggtca tatcgtattc gccaggctgt aaattcgccg tcattttctg gctaaagcca
    10021 ggggcgatat tttcccgctc ttccaccacc atcacgcctt tgaggatctc ccactccagc
    10081 gccttctggc tgtggttctg aataatgaac tgtgttttcc cggcgttaac cgtaatggtc
    10141 atcggttcgc actgcttatc ggtcacggtc accttgacct gcggcacatt agcggcgtta
    10201 gccataaaag cagaagagaa cagcgcagcc acgctcaact gcaatgcgtt acggcggaag
    10261 ttaatggtca tgacgagact atccctttaa agtatgttgt aactaagcaa gagtttgcgt
    10321 cgtatttgct acgcggagcg agacgctgtc gcccctgcgc gtggtggcag ggcaaatgcc
    10381 accagcgccg ggatgagata aataaaccag acggcgactt cgctgacgct cggtgcttcc
    10441 tgatagccaa aaatcccttc catcagcgtg ccaaacagcg agtgagttga gaggaccgcg
    10501 ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc cggcttcatg aaatgcgcga
    10561 atggcaccag ccgccagccc tgcggcgacg aagagaataa acaggctggt ccatttaaaa
    10621 aatgcaccaa gattgaggcg aataccgccc cagtagagca ggaagccgag caccacggca
    10681 gtagcaagac cgagcattgc acccagcggc ggccagatcc cgacatcttg ttgaaatgcc
    10741 gccagcagga aaaagaccga ctccagccct tcccttgcaa cggcaaaaaa gaccatcatc
    10801 accagcgccc agccatgatg atttccacgc tgcaatgcgc tatcgactgc ctgttccagt
    10861 tgcactttga cgttgcgcga cactttgcgc atccagaaaa ccatccaggt aaggatcacc
    10921 acggcgatca ccgccacgat accttcaaac agttcctgtt ctttttgcgg aaattcgccg
    10981 gtggtttcgt taatgaagat acccaggccc aggcacaacg cagcggcaag caacacgcca
    11041 atccacatca caccaatcca tcggcctcgc tgggtacgct taagatagct ggcaatcaaa
    11101 ctgacaatca gcgcggcttc aagtccttcg cgcaacataa tgagaaacgg aacaaacatg
    11161 ccacccaccc ttaaacgttt ttctgagtca atctatgcaa agaaaagtaa atcgcagtga
    11221 tagcgattat cattacggca aagaaaaata caagcggaat gtttataaaa tgatgttgga
    11281 atgtaaacaa agagaggtaa tgccaaggga tataaacgat tattccaggg tagaaagatg
    11341 ggattgtttg caaagaacca tgtgttcttg ccggatgccg cgtaaacgcc gtatccagcc
    11401 gacaaaactg tccagactca atgcatcaga gcttccatgt aaaactgata agcgggtgta
    11461 tcaagcaatt tcgtccatcc gcgattatca aatctaactt tttgcccgga acataataac
    11521 agctcgggtg ttggtggcgt caaaatcgct gaagcgttcc ccgaaggccg ggacagcagg
    11581 gggcggcggc gattggccgc cccctgcgcg ctccctgcac cattcaattt atatggcgag
    11641 gcacattaag ggagcgcaac ccgctgctga agccaaacaa aaccgccgca ggctaagtcc
    11701 cttagctttc ctgcaaccgt gacggcggag ccgaatgata gtgcgcatcg gcctcggcaa
    11761 agcgtttttg catcgccgct gacggcgctt tacccagcaa actaaacact acaatcccaa
    11821 tactgccgaa gataaagccc ggaataattt cgtagagtcc cagccagccg aactgcttcc
    11881 agacgataac cgtcagcgca ccgatgatca tccccgccag cgcaccgttg cgcgtcatgc
    11941 gtgaccacat caccgagaac agcaccaccg gaccaaacgc cgcgccaaag cctgcccacg
    12001 cgtagctcac taagcccagc acgcggtttt ccgggtttgc cgccagcgca atcgccacca
    12061 gcgccaccac cagcaccatc acacgcccta cccacaccag ctctttctgg ctggcatgtt
    12121 tacgcagaaa cgctttgtat aaatcttcgg taatcgcact ggagcacacc agcagctggc
    12181 agcttaacgt tgacattacc gccgccagaa ttgccgacag cagaatcccg gcaatccacg
    12241 ggttaaacag aatttgcgcc agttcgataa acacacgttc agcgttctgg tttaccgcac
    12301 ctgctaccgc cgggtgctca ttaaagtaag caatcccaaa gaagccgaca gccaccgccc
    12361 ctgccaggca gaggatcatc caggtcatac tgatacgacg cgcatggaca atgctgtggt
    12421 gagaatccgc cgccataaaa cgcgccagaa tatgaggctg accgaagtaa cccagccccc
    12481 aacccatcaa tgagataatg gcaacaaagt tcagcccttt gagcatatcc acgttttcga
    12541 tgctcttttg tttgatcact tccagcgagt caccaaagcc accgacactg ataatgacga
    12601 taaccggcgt caggatcagg gcaaaaatca tcaggctggc ctgtacagtg tcagtccagc
    12661 tcaccgcgag gaaaccgcca ataaaggtgt aaaggatcgt cgccgcagcg cccgcccaca
    12721 gagccgtttc gtagctcatg ccaaaggtac tttcaaacag acgcgcgcct gccacaatgc
    12781 ccgaagcgca ataaatggtg aagaacagca aaataaccag cgcagagata atgcgcaaaa
    12841 tgcggctttt atcttcaaag cgcccggtga aataatccgg caatgttaag gcgttattgt
    12901 tgtattcggt atgcacacgc aaccgcccgg ccaccagctt ccagttgatc cacgcgccta
    12961 atgtcaggcc aatggcgatc cagctttcgg aaatcccgga aagaaaaacc gcgcccggca
    13021 accccattaa cagccagccg ctcatatccg acgcacccgc cgataatgcc gtcacgaatg
    13081 gcccaagact acgaccgccc agaatatagt cgtcaaagtt tttcgttgat cgccaagcga
    13141 taaacccaat caatatcatg ccaaagatat agacacaaaa tgtcaccaac atcggtgtgc
    13201 taatagccat ctaaagtctc caaaaaatta ttatcggcaa tgtcgaaact tgccgttata
    13261 tctgccaccg gaacggggta acagagttta tgttttacca gggcgaccgt atcctgccgg
    13321 aagcgctggt tattcacaat cgatttaaca caccatttac attaaatttt agtgctcagc
    13381 gacactattt ttcatcaggt tgcactctct cacatttttt gcggttgcac ctttcaaaaa
    13441 tgttaactgc cgcagagaaa aagtctgagt tattttttaa tccctgtcat atcgatttct
    13501 tttattaaca tttcattcat ttttaagctt gctacgcagg tcacatttaa catggttgca
    13561 caaagttgca acatcatgga tatttcacga taacgttaag ttgcaccttt cagaacaaca
    13621 ggagtaatgg catgggaacc accaccatgg gggttaagct ggacgacgcg acgcgtgagc
    13681 gtattaagtc tgccgcgaca cgtatcgatc gcacaccaca ctggttaatt aagcaggcga
    13741 ttttttctta tcttgaacaa ctggaaaaca gcgatactct gccggagcta cctgcgctgc
    13801 tttctggcgc ggccaatgag agcgatgaag caccgactcc ggcagaggaa ccacaccagc
    13861 cattcctcga ctttgccgag caaatattgc cccagtcggt ttcccgcgcc gcgatcacag
    13921 cggcctatcg ccgcccggaa accgaagcgg tttctatgct gctggaacaa gcccgcctgc
    13981 cgcagccagt tgctgaacag gcgcacaaac tggcgtatca actggccgat aaactgcgta
    14041 atcaaaaaaa tgccagtggt cgcgcaggca tggttcaggg gttattgcag gaattttcgc
    14101 tgtcatcgca ggaaggtgtg gcgctgatgt gtctggcaga agcgttgttg cgtattcccg
    14161 acaaagccac ccgggatgca ttaattcgcg acaaaatcag caacggtaac tggcagtcac
    14221 acattggtcg tagcccgtcc ctgtttgtta atgccgctac ctgggggctg ctgtttaccg
    14281 gcaaactggt ttccacccat aacgaagcca gcctctcccg ctcgctgaac cgcattatcg
    14341 gtaagagcgg tgaaccgctg atccgcaaag gtgtggatat ggcgatgcgc ctgatgggcg
    14401 agcagttcgt cacgggtgaa accattgcag aagcgttagc caatgcccgc aagctggaag
    14461 agaaaggttt ccgttactct tacgatatgc ttggcgaagc tgcactgact gccgcagatg
    14521 cacaggcgta tatggtttcc tatcagcagg cgattcacgc cattggtaaa gcgtccaatg
    14581 gtcgtggcat ctatgaaggt ccgggcattt caatcaagct gtcggcgctg catccgcgtt
    14641 acagccgcgc ccagtatgac cgggtaatgg aagagcttta cccacgtctg aaatcactca
    14701 ccctgctggc gcgtcagtac gatattggta tcaacattga cgccgaagag gccgatcgtc
    14761 tggagatctc cctcgatctg ctggaaaaac tctgtttcga gcctgagctg gcaggctgga
    14821 acggtatcgg ttttgttatt caggcttatc aaaaacgctg cccactggtg attgattacc
    14881 tgattgatct cgccacccgc agccgtcgcc gtctgatgat tcgcctggtg aaaggcgcgt
    14941 actgggatag tgaaatcaag cgcgcgcaga tggacggcct tgaaggctat ccggtttata
    15001 cccgcaaggt gtataccgac gtttcatatc tcgcctgtgc gaaaaagctg ctggcggtgc
    15061 cgaatctaat ctacccgcag ttcgcgacgc acaacgccca tacgctggca gcgatttatc
    15121 aactggcagg tcagaactac tatccgggtc agtacgagtt ccagtgcctg catggtatgg
    15181 gcgagccact gtatgagcag gtcaccggga aagttgccga cggcaaactt aaccgtccgt
    15241 gtcgtattta tgctccggtc ggtacccatg aaacgctgtt ggcgtatctg gtgcgtcgcc
    15301 tgctggaaaa cggtgctaac acctcgtttg ttaaccgtat tgccgacacc tctttgccac
    15361 tggatgaact ggtcgccgat ccggtcactg ctgtagaaaa actggcgcaa caggaagggc
    15421 aaactggatt accgcatccg aaaattcccc tgccgcgcga tctttacggt cacgggcgcg
    15481 acaactcggc agggctggat ctcgctaacg aacaccggct ggcctcgctc tcttctgccc
    15541 tgctcaatag tgcactgcaa aaatggcagg ccttgccaat gctggaacaa ccggtagcgg
    15601 caggtgagat gtcgcccgtt attaaccctg cggaaccgaa agatattgtg ggctttgtgc
    15661 gtgaagccac gccgcgtgag gtagaacagg cgctggaaag tgcggttaat aacgcgccaa
    15721 tctggtttgc cactcctccg gttgaacgcg cggcgatttt gcatcgcgct gccgtgctaa
    15781 tggaaagcca gatgcagcaa ctgattggta ttctggtgcg tgaagccgga aaaaccttca
    15841 gtaacgccat tgccgaagtg cgcgaagcgg tcgattttct ccactactat gccggacagg
    15901 tgcgggatga tttcgctaac gaaacccacc gtccattagg gcctgtggtg tgtatcagtc
    15961 cgtggaactt cccgctggct attttcaccg ggcagattgc tgccgcactg gcggcaggta
    16021 acagcgtgct ggcaaaaccg gcagaacaaa cgccgctgat tgccgcgcaa gggatcgcca
    16081 ttttgctgga agcgggtgta ccgccaggcg tggtgcagtt gctgccaggt cagggggaaa
    16141 ccgtgggcgc gcaactgacg ggtgatgatc gcgtacgcgg ggtgatgttt accggttcaa
    16201 ccgaagtcgc tacgttactg cagcgcaata tcgccagccg cctggacgct cagggtcgcc
    16261 ctattccgct catcgctgaa accggcggca tgaacgcgat gattgtcgat tcttcagcac
    16321 tgaccgaaca ggtcgtcgtg gatgtactgg cctcggcgtt cgacagtgcg ggtcagcgtt
    16381 gttcggcgct gcgcgtgctg tgcctgcaag atgagattgc cgaccacacg ttgaaaatgc
    16441 tgcgcggcgc aatggccgaa tgccggatgg gtaatccggg tcgcctgacc accgatatcg
    16501 gtccggtgat tgatagcgaa gcgaaagcca atattgagcg ccatattcag accatgcgta
    16561 gcaaaggccg tccggtgttc caggcggtgc gggaaaacag cgaagatgcc cgtgaatggc
    16621 aaagcggcac ctttgtcgcc ccgacgctga tcgaactgga tgactttgcc gaattgcaaa
    16681 aagaggtctt tggtccggtg ctgcatgtgg tgcgttacaa ccgtaaccag ctaccagagc
    16741 tgatcgagca gattaacgct tccggctatg gtctgacgct tggtgtccat acgcgtattg
    16801 atgaaaccat cgcccaggtc actggctcgg cccatgttgg taacctgtat gttaaccgta
    16861 atatggtggg cgcagtggtg ggtgtgcagc cattcggcgg cgaagggttg tccggtaccg
    16921 ggccgaaagc aggcggtccg ctctatctct accgtctgct ggcgaatcgc ccggaaagtg
    16981 cgctggcagt gacgctcgcg cgtcaggatg cagagtatcc ggtcgatgca cagttgaaag
    17041 ccgcattgac tcagccgcta aatgcactgc gggaatgggc agcaaatcgt ccagaattgc
    17101 aggcgttatg tacgcaatat ggcgagctgg cgcaggcagg aacacaacga ttgctgccgg
    17161 ggccgacggg tgaacgcaac acctggacgc tgctgccgcg tgagcgcgtg ttgtgtattg
    17221 ccgatgatga gcaggatgcg ctgactcagc tcgccgccgt gctggcggtg ggcagccagg
    17281 tactgtggcc ggatgacgcg ctgcatcgtc agctagtaaa ggcattgcca tcggcagtca
    17341 gcgaacgtat tcaactggcg aaagcggaaa atataaccgc tcaacccttt gatgcggtga
    17401 tcttccacgg tgattcggat cagcttcgcg cattgtgtga agccgttgct gcgcgggatg
    17461 gcgcaattgt ttcggtgcag ggttttgccc gtagcgaaag caatatcctt ctggaacggc
    17521 tgtatatcga acgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagtttaa
    17581 tgacgatcgg ttaaacaggt agccggagga tgttacaacc tcctccggca tctttaacgt
    17641 ggtcgaatcc cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatacc
    17701 tcatcgcgca acgtcgcgcc tgtcaccgcc tccacctgag gggcaaaatc ggcgtaatgt
    17761 tgagtggaag cccaaatcat aaaaatcaaa tgctgcggat caatcggcgc gagtttgccg
    17821 cttttgaccc aaccggcaat cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc
    17881 gtcagttcat ccattaacag cggcgcgcct gccagcatct ccatacagaa caggcgcgaa
    17941 gcctgcggat aatcgcggga gacttccagc ttcagacgga tgtactcttt gatcgccgcc
    18001 agcggggcga aatcttctcg aaacgctttt aacggtgcca gccagatatc gagaatctgc
    18061 cgcagcacgg caatatacag cgcctctttt gacggaaagt aatacagcag attggttttt
    18121 gaaacacccg ccaactctgc gatctgctcc agccttgtgc cgtgaaaacc gaattgtgaa
    18181 aaagtgtcca gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt
    18241 ttacccgttg ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggcaac
    18301 aggatagcaa aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acagatgcat
    18361 gttttatgtg caactgtttt gaccgtttag tccacttttt accagatatt ttagataacg
    18421 cgttgttaac atattaacaa cattaacttt ttaaaaactg gcatccgctt tgcaaacaag
    18481 ccaatacgcg gctgaaaagg atatgcagga tgcagcgccc cgtctgactt tcactttacg
    18541 agacgaagag aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg
    18601 gctcatttcg acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccattgt
    18661 gcaaaaagcg gagcactacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt
    18721 tggcggcaaa actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcggggct
    18781 ggcggccgtg acctcgcgca ttcagattta cgccactgct gccaccttaa cgttacctcc
    18841 ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt
    18901 caacctcgtg actggctggc aaaagcccga gtatgagcag atgggtatct ggcctggcga
    18961 tgactatttc tcccgtcgtt acgactatct caccgaatat gttcaggtgc tgcgcgacct
    19021 gtggggcacg gggaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt
    19081 cagtccgcaa ccgagtgtcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat
    19141 ggcgttctcc gctcagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacacc
    19201 cacggctttc gccccgaccg ctgtgcggat gaaacaggcc gcagagcaaa ccgggcgcga
    19261 cgttggctct tatgtgttgt ttatggtgat tgccgatgaa accgacgatg ccgctcgcgc
    19321 caaatgggaa cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca
    19381 aagtcagaaa gatacccgct ccggtactga caccaacgtc cgtcagatgg ccgatcccac
    19441 ttcggcggta aacatcaata tggggacatt agtcggttct tacgccagtg tcgcgcgcat
    19501 gttagatgaa gtcgcaagcg tgcctggtgc cgaaggcgtg ctgttgacct tcgatgattt
    19561 tctgtcggga atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca
    19621 tctccctgcg ctgactcagg aggtggcatg atgacaacct taaccgctcg accggaagcc
    19681 attaccttcg atccgcagca aagtgcgctg atcgtggtgg atatgcaaaa cgcttatgcc
    19741 acgccaggcg gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt
    19801 gccaacattc aaaccgccgt gaccgcagcg cgagcggcag ggatgctgat catctggttt
    19861 caaaatggct gggatgaaca gtatgtcgaa gctggcggac ccggctcacc gaattttcat
    19921 aaatcgaacg ccctgaaaac catgcgtaag cagccgcagc tgcaggggaa attgctggcg
    19981 aaaggctcct gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg
    20041 ctgccgaagc cgcgctacag cggtttcttc aatacaccgc tggacagcat tttgcgcagc
    20101 cgtggaatac gccatctggt tttcaccggt atcgctacca acgtctgcgt cgaatcgacg
    20161 ctacgcgatg gcttttttct ggagtatttc ggcgtggtgc tggaagacgc aacacaccag
    20221 gcggggccgg aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg
    20281 gtcagcgacg tcgaaacgtt ctgcgacgcg ctttctccca catcctttgc tcgtatcgct
    20341 taaggagttt aacgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg
    20401 cccccttcgt tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt
    20461 ttgatcaaca taacaacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc
    20521 tggaaactat ccgcaaggtg atcgagacgg cgggtggcac gatggcggat gtgaccttca
    20581 acagcatctt tattaccgac tggaaaaatt acgccgcgat taacgaaatc tacgccgagt
    20641 tttttccggg tgataaaccg gcgcgattct gcattcagtg cggactggta aaacctgacg
    20701 cgctggtgga aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca
    20761 ctctcacctc ccccttatgc tgatgcgccc gtagtggtgt tgatttcggg tcttggcggt
    20821 agcggcagtt actggttacc gcaactggcg gtgctggagc aggagtatca ggtagtctgt
    20881 tacgaccagc gcggcaccgg caataatccc gacacactgg cagaagatta cagtatcgcc
    20941 cagatggcag cggaactgca tcaggcgctg gtagccgcag ggattgagca ttacgcggtg
    21001 gtcggccatg cgctcggtgc gctggtggga atgcagctgg cgctggatta tcccgcgtcg
    21061 gtaactgtgc tgatcagcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt
    21121 tttcaggttc gcgaacgatt actgtatagc ggcggcgcgc aggcatgggt ggaagcgcag
    21181 ccgttgttcc tctatcccgc cgactggatg gcggcccgcg tacctcgcct ggaggcagaa
    21241 gacgcgctgg cactggcgca ttttcagggc aaaaataatt tactgcgtcg acttaacgcc
    21301 ctcaaacgcg ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc
    21361 tgcgccagtg atgatctgct ggtgccaaca gcatgttcca gtgaacttca tgccgccctg
    21421 cccgatagcc agaaaatggt gatgccctat ggcggacacg cctgcaacgt gaccgatccc
    21481 gaaacgttta atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc
    21541 gccctgtaag gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccttgttc
    21601 accgatgccc gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacgg
    21661 gagatttatg ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacggatc
    21721 gtgtttatcc gcacggcaga aggaaaagaa cgtctgcgcc cggcactttc cagcggcaat
    21781 ctgcaaaaaa ccctgaccgc gcccgtcacc gctatcgtcg cctgggacag tgaattttat
    21841 gaacggttac cgcaactgtt tcctcacggc gatgcccgca gttggtttac ctccagccca
    21901 caacttgccg aagaaacagc gtttcgcaac agttccatgc aggcggccta tctgatcgtc
    21961 gcttgccgtg cgctgggact ggataccggc ccgatgtcgg gttttgaccg tcaacacgtt
    22021 gacgacgcct tttttgcggg cagcacgctg aagagcaatc tgctgattaa tatcggctat
    22081 ggcgatagca gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg
    22141 ctgttgtaag gagcaaacta tgaacatagt tgatcaacaa acctttcgcg atgcgatgtc
    22201 ctgtatgggc gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt
    22261 caccgccagc gccgtctgca gcgtgaccga cacgccgccc acattactgg tgtgcctgaa
    22321 tcgtggggcg tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact
    22381 gagcgccggg caggagccgc tttcaaacct ttttggcggc aaaacgccca tggaacaccg
    22441 ctttgccgcc gcccgctggc agacgggggt gaccggatgc ccgcaactgg aagaggctct
    22501 ggtttcgttt gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattctgtt
    22561 ttgcgccatc gaagcgattc atcgtcacac cacaccctac gggctggtgt ggttcgatcg
    22621 cagttatcac gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca
    22681 atgttcggtt ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg
    22741 ccggatgaac gattgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg
    22801 atgtttggcg cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt
    22861 ttaatgtcgg ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtccccagt
    22921 tatctcggtt ccagcgcggc ttttgtcggc gtggtgattg ccgcgacggg ctttaacggt
    22981 cagggcatta acccgaatat cagcattgcg ctgggcggga ttatcgcctg tgggctggtt
    23041 tataccgtca ttggcctggt ggtgatgaaa attggtacac gctggattga acgactaatg
    23101 ccgccggtgg tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgca
    23161 gtgaaaagtg tatcggcttc ggcctttgat agctggatgg cggtaatgac ggtgctgtgt
    23221 attgggctgg tggcggtgtt tacccgtggc atgatccagc ggttattgat tctcgtaggt
    23281 ttaattgtcg cctgtctgct gtatggcgtg atgaccaacc ttttagggct gggaaaagcc
    23341 gtcgatttta ctctcgtcag ccatgctgcc tggttcggtt tgccacattt ttccactcct
    23401 gcttttaata gtcaggcaat gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa
    23461 aacttaggtc atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg
    23521 gggcgggcat tcgtcggtga tgggctggcg acgatgcttt ctggttctgt cggtggcagc
    23581 ggcgtaacca cctacgccga gaatatcggt gtaatggccg tgacaaaagt ctactcgacg
    23641 ctggtgtttg tcgctgctgc cgtcatcgcc atgctgttgg gcttttcacc gaaatttggc
    23701 gcgctgattc ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg
    23761 ctgattgccg tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac
    23821 ggtaatttga ttatggtcgc agtaacctta gtgctgggcg cgggtgattt tgctctcacg
    23881 ctgggcggtt ttacgttggg agggattggt acagcaacct ttggcgcgat tttactcaat
    23941 gcgttgttaa gcagaaagtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca
    24001 taaacagaaa accgccggga ttaccggcgg tgatgacttt ttgacgagcc taagaaaaaa
    24061 atgcattcgt cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac
    24121 cttttttacc tgcttcagat gcgcgctgcg ggtcattctt gaaattaccc ccgctgtgct
    24181 gtccaccttt tttacctgct tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac
    24241 cgcctcgatg gtttgccatt actgacctcc gtctgggtat agacatcata ttgcatagaa
    24301 ttaacgcggc gtatggcggc tgccatattt tcgctacgtg ggactaagct tagtgaactg
    24361 acgcgggcag acccgcgttt ggtaatatcc tgcaacagct tcttagcgag aatagatatc
    24421 atctcaaata attatccata agccgctctt atgattaaaa aaacgcgatg agataaaaat
    24481 gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt
    24541 gtgaaatttg cactctgaaa aggacgtctt atctttaaat aagtggtagc gaatcgctac
    24601 ggaatagaga taacacgagg agtggttaga aatggctaaa gttctggtgc tttattattc
    24661 catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa gcaaagtcga
    24721 tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa
    24781 agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg ccgattacga
    24841 cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt
    24901 cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt
    24961 ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa cctggacgac
    25021 ccttgcgcat cacggcatgg taattgtccc cattggctac gcagcgcagg aattatttga
    25081 cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg
    25141 ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc
    25201 aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa
    25261 gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata
    25321 gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcggaaaa gatttgggaa
    25381 gaaggtagcg atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt
    25441 tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc acttctcgcg
    25501 ggggagtttt ctgttatttc accgcctcat tcaacacgct atcaaactta tccattgggc
    25561 agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacacgc tgagcaggtg
    25621 cctgcagggt taacgcatcg gcattacgta actgttccgc actctgatac acatattcaa
    25681 ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc
    25741 caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta
    25801 acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt gcgctggtgc
    25861 gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca
    25921 cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac aacaccttcc
    25981 actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtaat
    26041 attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacacctg
    26101 gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa cactgttgtt
    26161 tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagt tggtagctgt
    26221 cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt tcactgaatg
    26281 cggcggaatc atcggtgatc accgggttaa aggttgggtc catagtgccc attttttcct
    26341 ggtgatgcac aggaatatca caccccggga atgcgccggt aataaagaac tgtgcggtcg
    26401 caacggtacg ttgcagacta ttggcatagg cataaacggt gtccggcggc gggcattccc
    26461 ccgatttcac catcccctgc tgtgccagcc attcacgcat gtaatggccc atatacactt
    26521 cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattcg ggccatttat
    26581 tcggcgtaga ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc
    26641 ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcat
    26701 ttgaagcgag taaaactatc cctgccacag ttgcggcgat tagcgttttg ttcattcctg
    26761 gcacctcttt tgttatcaat cactaaagag tgtgacagaa atatgacatt tttccgggaa
    26821 caggttcgca aaatggcttg ttgaatgcct gtttttctat atacatcttt aaaaacaggc
    26881 aaggtttgac gcaaactatg cctgattagc tccctgcctc gcgtacgccg ctgatgatta
    26941 cacctgtggc taaacagaat atcagcggcg ttactaatac agaggcaata gtaccaataa
    27001 taagagaagt gaggctaata acaatggttt ttagcacatg cctttgtcag taaattgcat
    27061 aacgagttaa aaaatcaatt tgcgcattcc tttccttatt gtttaactcg taagcctgag
    27121 caaaaagttc aggagggagg taaacgtcat aatgattgcc agtgtttatt aaatacccat
    27181 actcaaggtc gtcatctgtt gaaaaggatt cattccctaa ggagattgat gccacattac
    27241 tattaacaat aaataatgag caaatgctat ctgaaagagc agtgaggaaa tttttcacct
    27301 cattaacctt ctgctctggg
//
LOCUS       DAPHJE010000060        26560 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPHJE010000060 DAPHJE010000000
VERSION     DAPHJE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.060"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..1567
                     /gene="gspA"
                     /locus_tag="RH222_002537"
     CDS             98..1567
                     /gene="gspA"
                     /locus_tag="RH222_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HDV3797539.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1569..1988
                     /gene="gspB"
                     /locus_tag="RH222_002538"
     CDS             1569..1988
                     /gene="gspB"
                     /locus_tag="RH222_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HDV3797540.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2226..2537
                     /gene="rpsJ"
                     /locus_tag="RH222_002539"
     CDS             2226..2537
                     /gene="rpsJ"
                     /locus_tag="RH222_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV3797541.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2570..3199
                     /gene="rplC"
                     /locus_tag="RH222_002540"
     CDS             2570..3199
                     /gene="rplC"
                     /locus_tag="RH222_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV3797542.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3210..3815
                     /gene="rplD"
                     /locus_tag="RH222_002541"
     CDS             3210..3815
                     /gene="rplD"
                     /locus_tag="RH222_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV3797543.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3812..4114
                     /gene="rplW"
                     /locus_tag="RH222_002542"
     CDS             3812..4114
                     /gene="rplW"
                     /locus_tag="RH222_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV3797544.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4132..4953
                     /gene="rplB"
                     /locus_tag="RH222_002543"
     CDS             4132..4953
                     /gene="rplB"
                     /locus_tag="RH222_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV3797545.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4970..5248
                     /gene="rpsS"
                     /locus_tag="RH222_002544"
     CDS             4970..5248
                     /gene="rpsS"
                     /locus_tag="RH222_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV3797546.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5263..5595
                     /gene="rplV"
                     /locus_tag="RH222_002545"
     CDS             5263..5595
                     /gene="rplV"
                     /locus_tag="RH222_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV3797547.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5613..6314
                     /gene="rpsC"
                     /locus_tag="RH222_002546"
     CDS             5613..6314
                     /gene="rpsC"
                     /locus_tag="RH222_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV3797548.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6327..6737
                     /gene="rplP"
                     /locus_tag="RH222_002547"
     CDS             6327..6737
                     /gene="rplP"
                     /locus_tag="RH222_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV3797549.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6737..6928
                     /gene="rpmC"
                     /locus_tag="RH222_002548"
     CDS             6737..6928
                     /gene="rpmC"
                     /locus_tag="RH222_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV3797550.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6928..7182
                     /gene="rpsQ"
                     /locus_tag="RH222_002549"
     CDS             6928..7182
                     /gene="rpsQ"
                     /locus_tag="RH222_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV3797551.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7347..7718
                     /gene="rplN"
                     /locus_tag="RH222_002550"
     CDS             7347..7718
                     /gene="rplN"
                     /locus_tag="RH222_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV3797552.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7729..8043
                     /gene="rplX"
                     /locus_tag="RH222_002551"
     CDS             7729..8043
                     /gene="rplX"
                     /locus_tag="RH222_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV3797553.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8058..8597
                     /gene="rplE"
                     /locus_tag="RH222_002552"
     CDS             8058..8597
                     /gene="rplE"
                     /locus_tag="RH222_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV3797554.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8612..8917
                     /gene="rpsN"
                     /locus_tag="RH222_002553"
     CDS             8612..8917
                     /gene="rpsN"
                     /locus_tag="RH222_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV3797555.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8951..9343
                     /gene="rpsH"
                     /locus_tag="RH222_002554"
     CDS             8951..9343
                     /gene="rpsH"
                     /locus_tag="RH222_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV3797556.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9356..9889
                     /gene="rplF"
                     /locus_tag="RH222_002555"
     CDS             9356..9889
                     /gene="rplF"
                     /locus_tag="RH222_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV3797557.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9899..10252
                     /gene="rplR"
                     /locus_tag="RH222_002556"
     CDS             9899..10252
                     /gene="rplR"
                     /locus_tag="RH222_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV3797558.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10267..10770
                     /gene="rpsE"
                     /locus_tag="RH222_002557"
     CDS             10267..10770
                     /gene="rpsE"
                     /locus_tag="RH222_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV3797559.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10774..10953
                     /gene="rpmD"
                     /locus_tag="RH222_002558"
     CDS             10774..10953
                     /gene="rpmD"
                     /locus_tag="RH222_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV3797560.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10957..11391
                     /gene="rplO"
                     /locus_tag="RH222_002559"
     CDS             10957..11391
                     /gene="rplO"
                     /locus_tag="RH222_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV3797561.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11399..12730
                     /gene="secY"
                     /locus_tag="RH222_002560"
     CDS             11399..12730
                     /gene="secY"
                     /locus_tag="RH222_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV3797562.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12762..12878
                     /gene="rpmJ"
                     /locus_tag="RH222_002561"
     CDS             12762..12878
                     /gene="rpmJ"
                     /locus_tag="RH222_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV3797563.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13025..13381
                     /gene="rpsM"
                     /locus_tag="RH222_002562"
     CDS             13025..13381
                     /gene="rpsM"
                     /locus_tag="RH222_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV3797564.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13398..13787
                     /gene="rpsK"
                     /locus_tag="RH222_002563"
     CDS             13398..13787
                     /gene="rpsK"
                     /locus_tag="RH222_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV3797565.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13821..14441
                     /gene="rpsD"
                     /locus_tag="RH222_002564"
     CDS             13821..14441
                     /gene="rpsD"
                     /locus_tag="RH222_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV3797566.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14467..15456
                     /gene="rpoA"
                     /locus_tag="RH222_002565"
     CDS             14467..15456
                     /gene="rpoA"
                     /locus_tag="RH222_002565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV3797567.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15497..15880
                     /gene="rplQ"
                     /locus_tag="RH222_002566"
     CDS             15497..15880
                     /gene="rplQ"
                     /locus_tag="RH222_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV3797568.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15987..16355
                     /locus_tag="RH222_002567"
     CDS             15987..16355
                     /locus_tag="RH222_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV3797569.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16366..16791
                     /gene="zntR"
                     /locus_tag="RH222_002568"
     CDS             16366..16791
                     /gene="zntR"
                     /locus_tag="RH222_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV3797570.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16847..17065
                     /gene="arfA"
                     /locus_tag="RH222_002569"
     CDS             16847..17065
                     /gene="arfA"
                     /locus_tag="RH222_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV3797571.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(17062..17472)
                     /gene="mscL"
                     /locus_tag="RH222_002570"
     CDS             complement(17062..17472)
                     /gene="mscL"
                     /locus_tag="RH222_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV3797572.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17602..18978)
                     /gene="trkA"
                     /locus_tag="RH222_002571"
     CDS             complement(17602..18978)
                     /gene="trkA"
                     /locus_tag="RH222_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV3797573.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19000..20289)
                     /gene="rsmB"
                     /locus_tag="RH222_002572"
     CDS             complement(19000..20289)
                     /gene="rsmB"
                     /locus_tag="RH222_002572"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV3797574.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20335..21282)
                     /gene="fmt"
                     /locus_tag="RH222_002573"
     CDS             complement(20335..21282)
                     /gene="fmt"
                     /locus_tag="RH222_002573"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV3797575.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21297..21806)
                     /gene="def"
                     /locus_tag="RH222_002574"
     CDS             complement(21297..21806)
                     /gene="def"
                     /locus_tag="RH222_002574"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV3797576.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21936..23060
                     /gene="dprA"
                     /locus_tag="RH222_002575"
     CDS             21936..23060
                     /gene="dprA"
                     /locus_tag="RH222_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV3797577.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23032..23505
                     /locus_tag="RH222_002576"
     CDS             23032..23505
                     /locus_tag="RH222_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV3797578.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23534..24076
                     /locus_tag="RH222_002577"
     CDS             23534..24076
                     /locus_tag="RH222_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV3797579.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24081..24653
                     /gene="tsaC"
                     /locus_tag="RH222_002578"
     CDS             24081..24653
                     /gene="tsaC"
                     /locus_tag="RH222_002578"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV3797580.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24658..25476
                     /gene="aroE"
                     /locus_tag="RH222_002579"
     CDS             24658..25476
                     /gene="aroE"
                     /locus_tag="RH222_002579"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV3797581.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKCNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25473..25730
                     /locus_tag="RH222_002580"
     CDS             25473..25730
                     /locus_tag="RH222_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV3797582.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25706..26260)
                     /locus_tag="RH222_002581"
     CDS             complement(25706..26260)
                     /locus_tag="RH222_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV3797583.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6786 a         6265 c         6939 g         6570 t
ORIGIN      
        1 agcaaaattt atttatatta gtaatatgta tttaattaat aaatacatac attattttta
       61 tgattaagga taatcaataa ttaaaacagg aagttctatg tctacgagaa gagaagttat
      121 tctttcctgg ttgtgtgaga aacgtcaaac ctggcgtcta tgctatttgt tgggtgaggc
      181 tggaagtgga aaaacctggc tggcgcagca actgcaaaaa gataaacatc gccgtgtgat
      241 tactttaagc ctcgttgttt cctggcaagg taaggccgca tggatcgtta ccgacgataa
      301 cgcggctgaa cagggctgcc gtgacagcgc ctggacgcga gatgagatgg cggggcaatt
      361 actgcatgcg cttcatcgaa ctgatagtcg ctgccctctt attattattg aaaatgctca
      421 cctgaatcac cgccgcatac ttgatgatct ccaacgcgca atatctctta ttcctgacgg
      481 tcaatttttg ctgatcggca gacccgatcg aaaagtcgaa cgtgatttta aaaaacaggg
      541 cattgaactt gtctcgatag gacgtctgac ggagcacgaa cttaaggcaa gcatccttga
      601 aggtcagaat attgaccaac ccgatctcct tttaaccgcc agagttctga aacggatagc
      661 tttattatgt cggggcgatc gcagaaagct ggcgcttgcc ggcgaaacaa ttcgcttatt
      721 acagcaagcc gagcagacca gcgttttcac agcaaaacaa tggcgaatga tttaccgcat
      781 attgggtgat aaccgccccc ggaaaatgca gcttgccgtg gtgatgagtg gaaccattat
      841 cgctcttacc tgtggttggc tactgctgtc ctcttttaca gctacgttac ccgttcctgc
      901 gtggctgata cccgtaacgc cagtagttaa acaagatatg actaaggata ttgctcatgt
      961 ggtgatgcgc gatagtgagg cgctaagcgt gttatacggc gtatgggggt acgaggtccc
     1021 agctgattcg gcctggtgcg atcaggcagt aagagcgggg ctggcatgta aaagtgggaa
     1081 tgcttcttta caaacattgg tagatcagaa tttgccctgg atcgcatcgc ttaaagtcgg
     1141 ggacaaaaaa ttgcctgttg tggtcgttcg cgtcggtgag gctagcgttg atgtcctcgt
     1201 aggtcagcaa acgtggacgc tgacacataa atggtttgag tcggtatgga cgggtgatta
     1261 tcttctgttg tggaaaatgt cgccggaagg ggagagtacc attacgcgtg atagcagcga
     1321 ggaagagatt ctctggctgg aaacgatgtt aaaccgcgca ttgcatattt cgacagaacc
     1381 ttcggcggag tggcgtccct tgttggtaga aaaaataaag caatttcaaa aaagccacca
     1441 cttgaaaact gacggtgttg ttggcttttc aacgttagta catctctggc aggtggcagg
     1501 ggagagtgcc tacttatatc gggatgaagc aaatatttcc ccggaaacaa cagtgaaggg
     1561 gaaatgacat gtttgaattc tatatcgccg cgcgtgagca gaaagaaaca ggtcaccctg
     1621 gtatcttctc ccgacaaaaa cacagcacca taatatatgt aatttgttta ttactgattt
     1681 gcctgtggtt tgcaggaatg gtgctggttg gtgggtatgc caggcagcta tgggtgcttt
     1741 ggatagtaaa agccgaagtc actgtagagg ctgaaacgcc cgcttttaaa caatcaacac
     1801 aacactattt cttcaaaaaa cagccactcc ccgtcgtcga atccgtcgag gaggaagatg
     1861 acccaggcgt cgcggttgag aatgccccct cttcttctga agacgaagaa aatactgtgg
     1921 aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa aaatgcactg aacgaacttg
     1981 agagataacc cgaaggctgt ttactttact agcaatacgc ttgcgttcgg tggttaagta
     2041 tgtataatgc gcgggcttgt cgtagttgac agcaggttca atctgaacct cagttagccg
     2101 aatttggcta cctaacaatg ctcccaatcg gggagctacg taagaacggt tacactctcc
     2161 catcaatcgt aatgggtctg aggagtaatc attttcgttt ataaaataat tggagctctg
     2221 gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg
     2281 atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac tggtgcgcag gtccgtggtc
     2341 cgatcccgct gccgacacgc aaagagcgct tcactgttct gatctccccg cacgtcaaca
     2401 aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg tctggttgac atcgttgagc
     2461 caaccgagaa aaccgttgat gctctgatgc gtctggatct ggctgccggt gtagacgtgc
     2521 agatcagcct gggttaatca ggtcattgag cgattgagag gttgaaacaa tgattggttt
     2581 agtcggtaaa aaagtgggta tgacccgtat cttcacagaa gacggcgttt ctatcccagt
     2641 aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg
     2701 ctaccgtgct attcaggtga ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga
     2761 agctggccac ttcgctaaag ctggcgtaga agctggccgt ggtctgtggg aattccgcct
     2821 ggctgaaggc gaagagttca ctgtaggtca gagcattagc gttgaactgt ttgctgacgt
     2881 taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg
     2941 ctggaacttc cgtacccagg acgctactca cggtaactcc ttgtctcacc gcgttccggg
     3001 ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca
     3061 gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta gtacgcgttg acgctgagcg
     3121 caacctgctg ctggttaaag gtgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa
     3181 accagctgtg aaggcgtaag gagatagcaa tggaattagt attgaaagac gcgcagagcg
     3241 cgctgactgt ttccgaaact accttcggtc gtgatttcaa cgaagcgctg gttcaccagg
     3301 ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg tgctcagaag actcgtgctg
     3361 aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg caccggccgt gcgcgttctg
     3421 gttctatcaa gagcccgatc tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg
     3481 accacagtca aaaagttaac aagaagatgt accgcggcgc gctgaaaagc atcctgtccg
     3541 aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa
     3601 ctaagctgct ggcacagaaa ctgaaagaca tggctctgga agatgtgctg atcatcaccg
     3661 gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct gcacaaggtt gacgtacgcg
     3721 atgcaactgg tatcgacccg gttagcctga tcgccttcga caaagtcgta atgactgctg
     3781 atgctgttaa gcaagttgag gagatgctgg catgattcgt gaagaacgtc tgctgaaggt
     3841 gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg atggaaaaat ccaacaccat
     3901 cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc aaagctgctg tgcagaaact
     3961 gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt aaagggaaag ttaaacgtca
     4021 cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg
     4081 ccagaatctg gacttcgttg gcggcgctga gtaagtcgga ggagtaatac aatggcagtt
     4141 gttaaatgta aaccgacatc tccgggtcgt cgccacgtag ttaaagtggt taaccctgag
     4201 ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt
     4261 aacaacaatg gccgtatcac cactcgtcat atcggtggtg gccacaagca ggcttaccgt
     4321 attgttgact tcaaacgcaa caaagacggt atcccggcag ttgttgaacg tcttgagtac
     4381 gatccgaacc gttccgcgaa catcgcgctg gttctgtaca aagacggtga acgccgttac
     4441 atcctggccc ctaaaggcct gaaagctggc gaccagattc agtctggcgt tgatgctgca
     4501 atcaaaccag gtaacaccct gccgatgcgc aacatcccgg ttggttctac tgttcataac
     4561 gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt ccgctggtac ttacgttcag
     4621 atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa
     4681 gtagaagcag actgccgtgc aactctgggc gaagttggca atgctgagca tatgctgcgc
     4741 gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc
     4801 gcgatgaacc cggtagacca cccacatggt ggtggtgaag gtcgtaactt tggtaagcac
     4861 ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact
     4921 gataaattca tcgtacgtcg ccgtagcaaa taattttaga ggataagcca tgccacgttc
     4981 tctcaagaaa ggtcctttta ttgacctgca cttgctgaag aaggtagaga aagcggtgga
     5041 aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat
     5101 gatcggtttg accatcgctg tccataatgg tcgtcagcac gttccggtat ttgtaaccga
     5161 cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact cgtacttatc gcggccacgc
     5221 tgctgataaa aaagcgaaga agaaataagg taggaggaag agatggaaac tatcgctaaa
     5281 catcgccatg ctcgttcttc tgctcagaag gttcgccttg ttgctgacct gattcgcggt
     5341 aagaaagtgt cgcaggctct ggatattttg acctacacca acaagaaagc ggctgtactg
     5401 gtcaagaaag ttctggaatc tgccattgct aacgctgaac acaacgatgg cgctgacatt
     5461 gacgatctga aagttacgaa aattttcgta gacgaaggcc cgagcatgaa gcgcattatg
     5521 ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca ccagccacat cactgtggtt
     5581 gtgtccgatc gctgagactc tggagactag caatgggtca gaaagtacat cctaatggta
     5641 ttcgcctggg tattgtaaaa ccatggaact ctacctggtt tgcgaacacc aaagaattcg
     5701 ctgacaacct ggacagcgat tttaaagtac gtcagtacct gactaaggaa ctggctaaag
     5761 cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag catccgtgta accattcaca
     5821 ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg
     5881 tcgtagcgga catcgctggc gttcctgcac agatcaacat cgccgaagtt cgtaagcctg
     5941 aactggacgc aaaactggtt gctgacagca tcacttctca gctggaacgt cgcgttatgt
     6001 tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg tctgggcgct aaaggtatta
     6061 aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc acgtaccgaa tggtaccgcg
     6121 aaggtcgcgt accgctgcac actctgcgtg ctgacatcga ctacaacacc tctgaagcgc
     6181 acaccactta cggtgtaatc ggcgttaaag tgtggatctt caaaggcgag atcctgggtg
     6241 gtatggctgc tgttgaacaa ccggaaaaac cggctgctca gcctaaaaag cagcagcgta
     6301 aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa
     6361 tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga tgttagcttc ggcagcttcg
     6421 gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca gatcgaagca gcacgtcgtg
     6481 ctatgacccg tgcagttaag cgtcaaggta agatctggat ccgtgtgttc ccggacaaac
     6541 cgatcactga aaagccgctg gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt
     6601 gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc
     6661 tggcccgtga agcattcaag ctggcagcag cgaaactgcc gattaaaacc acctttgtaa
     6721 ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag aagagcgttg aagagctgaa
     6781 caccgagctg ctgaacctgc tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg
     6841 ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac
     6901 tttactgaac gagaaggcgg gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc
     6961 gttgttagcg acaaaatgga gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac
     7021 ccgatctacg gtaaattcat caagcgtacg accaaactgc acgtacatga cgagaacaac
     7081 gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa
     7141 tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt aatacagtac actctctcga
     7201 tacgaataaa cggctcagaa atgagccgtt tattttttct acccatatcc ttgaagcggt
     7261 gttataatgc cgcgccctcg atatggggat ttttaacgac ctgattttcg ggtctcagta
     7321 gtagttgaca ttagcggagc actaaaatga tccaagaaca gactatgctg aacgtcgccg
     7381 acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct gggtggctcg caccgtcgct
     7441 acgcaggcgt aggcgacatc atcaagatca ccatcaaaga agcaattccg cgtggtaagg
     7501 tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc
     7561 cggacggttc tgtcattcgc ttcgatggta atgcttgtgt tcttctgaac aacaacagcg
     7621 agcagcctat cggtacgcgt atttttgggc cggtaactcg tgagcttcgt agtgagaagt
     7681 tcatgaaaat tatctctctg gcaccagaag tactctaagg agcgaatcat ggcagcgaaa
     7741 atccgtcgtg atgacgaagt tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa
     7801 gttaagaatg tcctgtcttc cggcaaggtc attgttgaag gtatcaacct ggttaagaaa
     7861 catcagaagc cggttccggc cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct
     7921 attcaggttt ccaacgtagc aatcttcaat gcggcaaccg gcaaggctga ccgtgtaggc
     7981 tttagattcg aagacggcaa aaaagtccgt ttcttcaagt ctaacagcga aactatcaag
     8041 taatttggag tagtacgatg gcgaaactgc atgattacta caaagacgaa gtagttaaaa
     8101 aactcatgac tgagtttaac tacaattctg tcatgcaagt ccctcgggtc gagaagatca
     8161 ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa actgctggat aacgcagcag
     8221 cagacctggc agcaatctcc ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg
     8281 caggcttcaa aatccgtcag ggctatccga tcggctgtaa agtaactctg cgtggcgaac
     8341 gcatgtggga gttctttgag cgcctgatca ctattgctgt acctcgtatc cgtgacttcc
     8401 gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc
     8461 agatcatctt cccagaaatc gactacgata aagtcgaccg cgttcgtggt ttggatatta
     8521 ccattaccac tactgcgaaa tctgacgaag aaggccgcgc tctgctggct gcctttgact
     8581 tcccgttccg caagtaaggt agggttacta aatggctaag caatcaatga aagcacgcga
     8641 agtaaaacgc gtagctttag ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat
     8701 catctctgat gtgaacgctt ccgacgaaga tcgttggaac gctgttctca agctgcagac
     8761 tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgtcc
     8821 gcatggtttc ctgcggaagt tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg
     8881 cggtgaaatc ccgggtctga aaaaggctag ctggtaattg tcaccaattg aatcacggga
     8941 gtaaagacag atgagcatgc aagatccgat cgcggatatg ctgacccgta tccgtaacgg
     9001 tcaggccgcg aacaaagctg cggtcaccat gccttcctcc aagctgaaag tggcaatcgc
     9061 caacgtgctg aaggaagaag gttttattga agattttaaa gttgaaggcg acaccaagcc
     9121 tgaactggaa cttactctga agtatttcca gggcaaagct gttgtagaaa gcattcagcg
     9181 tgtcagccgc ccaggtctgc gcatctataa acgtaaagat gagctgccga aagttatggc
     9241 gggtctgggt atcgcagttg tttctacctc taaaggtgtt atgactgatc gtgcagcgcg
     9301 ccaggctggt cttggtggcg aaattatctg ctacgtagcc taatcggagg aaaaaatgtc
     9361 tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca
     9421 ggttattacg atcaaaggta aaaacggcga gctgactcgt actctcaacg atgctgttga
     9481 agttaaacat gcagataata ccctgacctt cggtccgcgt gatggttacg cagacggttg
     9541 ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg gttatcggtg ttaccgaagg
     9601 cttcactaag aagctgcagc tggttggtgt aggttaccgt gcagcggtta aaggcaatgt
     9661 gattaacctg tctctgggtt tctctcatcc tgttgaccat cagctgcctg cgggtatcac
     9721 tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc gctgataagc aggtgatcgg
     9781 ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag ccttataaag gcaagggtgt
     9841 tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag aagaagtaag gtaacactat
     9901 ggataagaaa tctgctcgta tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct
     9961 gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac atttacgcac aggtaattgc
    10021 accgaacggt tctgaagttc tggtagctgc ttctactgta gaaaaagcta tcgctgaaca
    10081 actgaagtac accggtaaca aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg
    10141 cgctctggaa aaaggcatca aagatgtatc ctttgaccgt tccgggttcc aatatcatgg
    10201 tcgtgtccag gcactggcag atgctgcccg tgaagctggc cttcagttct aaggtagagg
    10261 tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc aggaaaagct gatcgcggta
    10321 aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct ccttcacagc tctgactgta
    10381 gttggcgatg gtaacggtcg cgttggtttt ggttacggta aagcgcgtga agttccagca
    10441 gcgatccaga aagcgatgga aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac
    10501 ggcactctgc aacaccctgt taaaggtgtt cacacgggtt ctcgcgtatt catgcagccg
    10561 gcttccgaag gtaccggtat catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct
    10621 ggggttcata acgttctggc taaagcatat ggttccacca acccgatcaa cgtggttcgt
    10681 gcaactattg atggcctgga aaatatgaat tctccagaaa tggtcgctgc caagcgtggt
    10741 aaatccgttg aagaaattct ggggaaataa accatggcaa agactattaa aattactcaa
    10801 acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa cgctgcttgg cctgggtctg
    10861 cgtcgtattg gtcacaccgt agagcgcgag gatactcctg ctattcgcgg tatgatcaac
    10921 gcggtttcct tcatggttaa agttgaggag taagagatgc gtttaaatac tctgtctccg
    10981 gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg gtatcggttc tggcctcggt
    11041 aaaaccggtg gtcgtggtca caaaggtcag aaatctcgtt ctggcggtgg cgtacgtcgc
    11101 ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct
    11161 cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc tggctaaagt agaaggcggt
    11221 gttgtagacc tgaacacgct gaaagcggct aacattatcg gtatccagat cgagttcgcg
    11281 aaagtgatcc tggctggcga agtaacgact ccggtaactg ttcgtggcct gcgtgttact
    11341 aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa tcgaggaata agtagcagat
    11401 ggctaaacaa ccgggattag attttcaaag tgccaaaggt ggcttaggcg agctgaaacg
    11461 cagactgctg tttgttatcg gtgcgctgat tgtgttccgt attggctctt ttattccgat
    11521 ccctggtatt gatgccgctg tacttgccaa actgcttgag caacagcgag gcaccatcat
    11581 tgagatgttt aacatgttct ctggtggtgc tctcagccgt gcttctatct ttgctctggg
    11641 gatcatgccg tatatttcgg cgtcgatcat tatccagctg ctgacggtgg ttcacccaac
    11701 gttggcagaa attaagaaag aaggggagtc tggtcgtcgt aagatcagcc agtacacccg
    11761 ttacggtact ctggtgctgg caatattcca gtcgatcggt attgctaccg gtctgccgaa
    11821 tatgcctggt atgcaaggcc tggtgattaa cccaggcttt gcattctact tcaccgctgt
    11881 tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg ggcgaacaga ttactgaacg
    11941 aggtatcggc aacggtattt caatcattat cttcgccggt attgtcgcgg gactcccgcc
    12001 agccattgcc catactatcg agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct
    12061 gttggttgca gtattagtat ttgcagtgac gttctttgtt gtatttgttg agcgtggtca
    12121 acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt cgtcgtgtct atgctgcaca
    12181 gagcacacat ttaccgctga aagtgaatat ggcgggggta atcccggcaa tcttcgcttc
    12241 cagtattatt ctgttcccgg cgaccatcgc gtcatggttc gggggcggta ctggttggaa
    12301 ctggctgaca acaatttcgc tgtatttgca gcctgggcaa ccgctttatg tgttactcta
    12361 tgcgtctgca atcatcttct tctgtttctt ctacacggcg ttggttttca acccgcgtga
    12421 aacagcagat aacctgaaga agtccggtgc atttgtacca ggaattcgtc cgggagagca
    12481 aacggcgaag tatatcgata aagtaatgac ccgcctgacc ctggttggtg cgctgtatat
    12541 tacctttatc tgcctgatcc cggagttcat gcgtgatgca atgaaagtac cgttctactt
    12601 cggtgggacc tcactgctta tcgttgttgt cgtgattatg gactttatgg ctcaagtgca
    12661 aactctgatg atgtccagtc agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta
    12721 cggccgataa ttggtcgccc gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg
    12781 tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt
    12841 gcagtgccga gccgaagcat aaacagcgcc aaggctgatt ttttcgcata tttttcttgc
    12901 aaagttgggt tgagctggct agattagcca gccaatcttt tgtatgtctg tgcgtttcca
    12961 tttgagtatc ctgaaaacgg gcttttcagc atggaacgta catattaaat agtaggagtg
    13021 catagtggcc cgtatagcag gcattaacat tcctgatcat aagcatgccg taatcgcatt
    13081 aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc atcctggctg cagcgggtat
    13141 cgctgaagat gttaagatca gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga
    13201 agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg
    13261 cctgatggat cttggttgct atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg
    13321 tcagcgtacc aagaccaacg cacgtacccg taagggtccg cgcaaaccga tcaagaaata
    13381 atcggggtga ttgaataatg gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac
    13441 aagtctctga cggcgtggct catatccatg cttctttcaa caacaccatc gtgactatca
    13501 ctgatcgtca gggtaacgcg ttgggttggg caacagccgg tggttccggt ttccgtggtt
    13561 ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga gcgttgcgct gacgccgtga
    13621 aagaatacgg catcaagaat ctggaagtta tggttaaagg tccgggtcca ggccgcgaat
    13681 ctactattcg tgctctgaac gccgcaggtt tccgcatcac taacattact gatgtgactc
    13741 cgatccctca taacggttgt cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc
    13801 aggtttgttg gagaaagaaa atggcaagat atttgggtcc taagctcaag ctgagccgtc
    13861 gtgagggcac cgacttattc cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa
    13921 ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg tctgtctgac tatggtgtgc
    13981 agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt cagttccgta
    14041 actactacaa agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc
    14101 tggaaggtcg tctggacaac gttgtatacc gtatgggctt cggtgccact cgtgcagaag
    14161 cacgtcagct ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt
    14221 cttatcaggt tagtccgaat gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc
    14281 gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa gccaacctgg ctggaagttg
    14341 atgctggcaa gatggaaggt acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg
    14401 acattaacga acacctgatc gtcgagcttt actccaagta aagcttagta ccaaagagag
    14461 gacacaatgc agggttctgt gacagagttt ctaaaaccgc gcctggttga tatcgagcaa
    14521 gtgagttcga cgcacgccaa ggtgaccctt gagcctttag agcgtggctt tggccatact
    14581 ctgggtaacg cactgcgccg tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag
    14641 gttgagattg atggtgtact acatgagtac agcaccaaag aaggcgttca ggaagatatc
    14701 ctggaaatcc tgctcaacct gaaagggctg gcggtgagag ttcagggcaa agatgaagtt
    14761 attcttacct tgaataaatc tggcattggc cctgtgactg cagccgatat cacccacgac
    14821 ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc acctgaccga tgagaacgcg
    14881 tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt atgtgccggc ttctacccga
    14941 attcattcgg aagaagatga gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc
    15001 cctgtggagc gtattgccta caatgttgaa gcagcgcgtg tagaacagcg taccgacctg
    15061 gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt
    15121 cgtgcggcaa ccattctggc tgaacaactg gaagctttcg ttgacttacg tgatgtacgt
    15181 cagcctgaag tgaaagaaga gaaaccagag ttcgatccga tcctgctgcg ccctgttgac
    15241 gatctggaat tgactgtccg ctctgctaac tgccttaaag cagaagctat ccactatatc
    15301 ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa
    15361 tctcttactg agattaaaga cgtgctggct tcccgtggac tgtctctggg catgcgcctg
    15421 gaaaactggc caccggcaag catcgctgac gagtaaccgg atcacaggtt aaggttttac
    15481 tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt caactgaacc gcaacagcag
    15541 ccatcgccag gctatgttcc gcaatatggc aggttcactg gttcgtcatg aaatcatcaa
    15601 gacgactctg cctaaagcga aagagctgcg ccgcgtagtt gagccgctga ttactcttgc
    15661 caagactgat agcgttgcta atcgtcgtct ggcattcgcc cgtactcgtg ataacgagat
    15721 cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg agccgtgccg gtggttacac
    15781 tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg ccgatggctt acatcgagct
    15841 ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa tctgaagcaa cgtaaaaaaa
    15901 cccgccccgg cgggtttttt tatacccgta gcatccccac ttatctacaa tagctgtact
    15961 ctttttgttc atcccctgga gtatttatgt ggttacttga ccagtgggca gagcgtcata
    16021 tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc aggtagcggc gaaccattga
    16081 tactggatga tgattctcac gtgccaccgg aattacgtgc ggggtatcgc ttgctgaaga
    16141 atgccggttg cttaccgcca gaacttgagc aacggagaga agcaattcag cttctggata
    16201 tcctgagagg tatccgtcac gatgatccgc aatatcaaga ggttagccgt cgattgtcat
    16261 tactggaatt gaagctgcga caagctggat tgagtaccga ttttttacgc ggcgattatg
    16321 ctgacaagtt gttggacaaa atcaacgata actagtggag tatgtatgta tcgcattggt
    16381 gagctggcaa aaatggcgga agtaacacct gacacgattc gttattacga aaaacagcag
    16441 atgatggagc atgaagtgcg tactgaaggc gggtttcgcc tgtataccga aagcgatctc
    16501 cagcgattga aatttatccg ccatgccaga caactaggtt tcagtctgga gtcgatccgc
    16561 gagttgctgt cgatccgcat cgatcctgaa caccatacct gtcaggagtc aaaaggcatt
    16621 gtgcaggaaa gattgcagga agtcgaagca cggatagccg agttgcagag tatgcagcgt
    16681 tccttgcaac gccttaacga tgcctgttgt gggaccgccc atagcagtgt ttattgttcg
    16741 attcttgaag ctcttgaaca aggggcgagt ggcgttaaga gtggctgttg attttttgca
    16801 ctggcaggat tacactcgcg ccgtaaaata accaattgga gtttttatga gccgatatca
    16861 gcatactaaa gggcagataa aggataatgc gatagaagca ttactacatg atcccttatt
    16921 ccgacagcgc gtagagaaaa ataagaaggg gaaaggcagt tacatgcgaa aaggaaaaca
    16981 tggcaatcgg gggaactggg aggccagtgg caagaaagta aatcactttt ttaccactgg
    17041 ccttctgctt tcaggcgttt gttaagagcg gttattctgc tctttcagca aatcacgaat
    17101 ttctgtcagt aatacttctt ctttagttgg tgctggtgcg gctgctggtt cttctttttt
    17161 ccgattcagt ttgttgatta gcttaatcgc cataaagatg gcaaaggcca caatcagaaa
    17221 atcaaagacg ttttgaatga agacaccgta atgcatcaca acagcaggga tatccccctg
    17281 cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc ccgccaatta ataagcccag
    17341 aggtggcatg atgatatcgg caaccagtga agatacaatc ttcccgaatg ccgcaccgat
    17401 aatgacaccc accgccaaat ccaccacgtt cccgcgcatc gcaaattcgc gaaattcttt
    17461 aataatgctc atgttattct ccctatgaag ccgacaacca taagtctaac aaatgttaag
    17521 ccattttcct gccaggaatg aatttaatta atccttgata atcaaaggga aataataagg
    17581 cgtcattaga cgccttatta attacaagaa gaaagggctt ggctggaaga gtctttcgac
    17641 gtcggtaata aactttttat ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg
    17701 cagattgtca ttggcaatca tcacgtcgtt tccacgtacc accgctccaa taatcgttcc
    17761 tggcggtagc ttgatttcgt caatgactct gccgacaacg cgtgaggtgc tttcatcacc
    17821 gtgagcaacg gcttcaatag cttctgctac gccgcggcgc aatgaggaaa caccaacaat
    17881 atctgctttt cgcacatggc taagcaacgc agaaatagtt gcttgttgtg gtgaaatcgc
    17941 aatatcgatc acgctcccct gaaccagatc cacataagcg cgacgctgga tcaataccat
    18001 cacctttttc gcacccatac gtttggcaag catggcggac atgatattgg cctcgtcatc
    18061 gttggtgacg gcaataaaca gatcaacttg atcgatatgt tcttcggcca gcagttcttg
    18121 atccgacgca tcaccaaaaa agacgatcgt attctgcaac ttttccgcca gttcggcagc
    18181 gcgctgctga ttacgttcga tgagtttaac gctgtaatct ttttccagac gacgcgccag
    18241 ccctgcaccg atattaccgc caccaaccag catgatccgc ttatacggtt tttccagtcg
    18301 ctgtaattca ctcatcaccg cacggatatg ctgtgaagcg gcaataaaga acacttcatc
    18361 accagcttcc acaatggtcg aaccttgtgg acgaatgggg cgatcgtggc ggaaaattgc
    18421 tgccacacga gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg cgttaccaat
    18481 cagcgggccg ccataatagg ctttaaccac agcgaggctg actttaccct cagcgaagtt
    18541 caccacctgc aatgcgccgg gatactcaat cagtcggtaa atattatcga taaccaactg
    18601 ctctggtgcg atcagatgat caatcggtac agcatctgaa tgaaatagct tatcggcatc
    18661 gcgcacgtag tctggtgagc ggatgcgagc gatgcgatta ggggtgttga aaagtgagta
    18721 ggctacctgg caggcaacca tattggtttc atctgaactg gttacagcaa ccagcatatc
    18781 ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga gagccatgcc cctgcacgac
    18841 tcgcaggtca aatttatcct gcaaggtccg cagacgctca ccgttggtat cgacaacagt
    18901 gatatcgttg ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct ggccggcacc
    18961 cagaatgata attttcatca gtcgcgaccc gttatctcat cactttttga ttagcttagc
    19021 gtaaaagaag ccgtcgccct cttcggtacc aggcagattt tgtttacccg gttgctctgg
    19081 tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc aaaaaggctt taatctgcag
    19141 gctattctct tccggtaaca ccgaacaggt ggcatagacc agggttccac cggattttaa
    19201 atgcggccaa atggcgtcga gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg
    19261 atcgcgacgt aaccatttaa tatccggatg gcgacgaatc acaccggttg ctgaacaagg
    19321 cgcatctaat aaaatgcgat caaactgttg ctcgccacac cattgggaag ggtagcggcc
    19381 atcaccttgt ttcacggtcg ctttcatacc aaggcgtttt aaattgtcgt aaacgcgaga
    19441 gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct tctggtgcca cctcaaggat
    19501 atgcgttgtt ttaccgccgg gggcggcaca aagatccaga atgtgttcac cgttttgtgg
    19561 cgcaagccag gtcatgcaac cttgtgctga tgcatcctga acggtaaccc atccgtcttc
    19621 aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc cgtacagcat cagggtaatc
    19681 cgcatgcggg aaacctttca ttcctgcttc atccaacaat gcaagccagc tgtcgcggga
    19741 atgatgcgta cgattaacgc gcagccacat tggcggacgc tggttattgg cttcgacgat
    19801 ggattgccac tgctctggat acgctttttg cagacgcttc agcaaccagg aaggatgcag
    19861 ataacgtgca tcactggcat taaactcggc taataactct tcttgctgac gctggaactg
    19921 gcgtaataca ccgtttatca accctttaag ttgcggacgc ttaattgcga tagcgccttc
    19981 aaccgtttca gccagcgcag catgaggtgg aatgcgggta taaagcagtt gatacaaacc
    20041 aaccataatc aggtaatgca cggtccgctg tttgccggtc atcggacggg ccattaactt
    20101 attaatcagc cagtctaact gcgaaagcgt acgcagtacg ccaaagcaca actcttgaag
    20161 aagtgctttg tctttatcgg aaactttttg ctggagcggt ggcagaatgt tgcttaatga
    20221 ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc atgctacgta aattacgttg
    20281 ttttttcata agtataaaaa tgcccggcaa gaccgggctt agaagagtgg actatcagac
    20341 cagacggttg cccggaacaa accattcccg acgagagttc aggaggtctt gcgcgctcat
    20401 cgctttctta cccgcaggtt gtaacgagag caggttcagg atgccatcac cagtcgcaac
    20461 ctgaatgcct tgtttattgg cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc
    20521 aatgaccgac gctttccaga ctttaaccgg ttgtccttca atttccagcc agctcattgg
    20581 ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct gccgaaagcg accagtcaat
    20641 acgcgcttct tctttactca acttctcggc gtaagtgaca agagtttcgt cctgaacttc
    20701 tggtttcgcc gtgccgtctg ccagttgttt caacgtggtg ataagccctt gtgggccaag
    20761 ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg ggcaggagag
    20821 cttatagagc atatcaccgg tgtctaaacc gacatccatt tgcataatgg tcacaccagt
    20881 ttctgcatca cccgcccata gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag
    20941 cagtgaacca tgaacgttga tacagccaag acgcggcatc tccagcactg ctttaggcag
    21001 aattaagcca taggcgacga cgaccataac atcagcctgc agatcggcga ccagttgctg
    21061 gttttcttgt ggacgcaggg aaacaggttg aaaaacgggc agaccttttt cctcagccag
    21121 aactttaacc gggctgggca tcagtttttt accgcgtcct gccggtcggt ctggctgggt
    21181 gaacacgcca acgacgttat gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa
    21241 gtcaggtgta cccgcaaaaa taatacgtag tgattctgac acgttagttc ttatccttaa
    21301 gcccgggctt tcagacgatc cagtttttca actttctgac gaatacgttg ttgtttcaac
    21361 ggtgacagat aatccataaa cagtttgccg accaggtgat ccatctcatg ctgaatacag
    21421 atggctaaca gaccgtctgc ttccagttca aatggtttac cgtcgcggtc cagggcgcga
    21481 attttaactt tctctgcgcg tggcactaaa gcacgttgtt cagggatcga caggcaacct
    21541 tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg gattgattaa caccagccgt
    21601 tcgtcacggt tttccgaaac atcaataaca atgatacgtt gatggatatc aacctgggtt
    21661 gccgccaggc caataccttc ttctgcgtac atcgtctcga acatatcatc gacgatacgc
    21721 tgaatttctg cattcacttc ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga
    21781 atatgtaaca cttgcaaaac tgacataaat ctccagagat gtgttcagga gttagaaaga
    21841 ttatttcttc tattctagac aaatccccct ctgattgaca gcatcactga ccaatcgcaa
    21901 agattgctaa ggctgcttat ggcagggaga taaggatggt cgatacagat atttggctgc
    21961 gtttaatgag catcagcagc ttgtacggcg atgatatggt ccgtatagct cactggctgg
    22021 caaaacagtc gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg
    22081 cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag ctcactttgt tggttggagc
    22141 aacccaacca tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttctggtga
    22201 cgacagatta ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc
    22261 agcttgccgt agtggggagt cgggcgcatt catggtatgg cgagcgatgg ggacggttat
    22321 tttgcgaaac tctggcgacg cgtggagtga caattacgag tggactggcg cgtggaatcg
    22381 atggtgtggc gcataaagct gccttacagg taaatggcgt cagcattgct gtattgggga
    22441 atggacttaa taccattcat ccccgccgcc atgcccgact ggctgccagt ctgcttgaac
    22501 atggcggagc tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc
    22561 cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt
    22621 tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg
    22681 ccttgccagg tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag
    22741 gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact
    22801 ggttgccaga cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc
    22861 catttcctga gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg
    22921 aacgtgccgg ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag
    22981 gatggatcgc agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac
    23041 gtactaatgt atttgtttga aacctatatt cacacagaag ctgagttgcg tgtggatcaa
    23101 gacaaacttg aacaggatct taccgacgca ggatttgatc gagaagatat ctacaatgcc
    23161 ctgctttggc tggaaaagct tgcggattat caggaaggac tggcagaacc gatgcaactg
    23221 gcctctgatc cactctccat gcgtatttat acccctgagg agtgtgaaag actggatgcc
    23281 agctgtcgtg gttttctgct tttccttgag caaattcagg tgcttaacct tgaaacccgt
    23341 gaaatggtga tagagcgagt gctcgcgctg gataccgcag agttcgacct ggaagacctg
    23401 aaatgggtaa tcctgatggt gttgttcaat attccgggct gcgaaaatgc gtaccagcaa
    23461 atggaagaat tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc
    23521 agcataagtt gttatggcga aatcagcact gttcacggtg cgtaataatg agtcctgccc
    23581 aaagtgcggg gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc
    23641 acagtatccg gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt
    23701 caaagttctg gaggggcagg tttgccttgc atgtggcgca aatctggtat tacgccaggg
    23761 acgctttggt atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat
    23821 cgataaaccg gacgaaacag caattacatg cccccaatgc cggacgggcc atctggtcca
    23881 gcgccgctcc cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt
    23941 tgccattaac ttcaaaccca tagctggaga atgccctgag tgtcattatc cactactcat
    24001 cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc
    24061 ggtttcggcg gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga
    24121 tagatgtcct caatgaagaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgttg
    24181 ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg
    24241 ttgataaggg gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg
    24301 acaccatgct gactgatgcg cagcgtgaaa ccattttttc ccgctggcca ggtcctgtca
    24361 cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc
    24421 ttgctgtacg agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac
    24481 cgctggtttc taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag
    24541 ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa
    24601 atccttcaga aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg
    24661 gaaacctatg ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag
    24721 caatttgctc aacaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat
    24781 gatttcatca acacactgaa cgctttcttt agtgctggtg gtaaaggtgc gaatgtgacg
    24841 gtacctttta aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg
    24901 gctggtgctg ttaataccct caagcggtta gaagatggac gcttgcaggg tgacaatacc
    24961 gatggtatag gtttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt
    25021 attctgctta tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg
    25081 gactgtgcgg tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg
    25141 tttgcgcaca ctggtagtat tcaggcgttg agtatggacg aactggaagg acatgagttt
    25201 gatctcatta ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca
    25261 tcgctcattc atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct
    25321 tttctggcat ggtgtgagca gcgaggctca aagtgtaatg ctgatggttt aggaatgctg
    25381 gtggcacagg cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca
    25441 gttataaagc aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca
    25501 gagaagagtg ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggcatgc
    25561 aactgacatg tgcgatctct ggcgagagtc tggcgtatcg ttttactgga gatacgccag
    25621 aacagtggtt agcgagtttt cgtcagcatc gctgggatct ggaagaagaa gcggaaaact
    25681 taattcagga acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt
    25741 cgtccttcca tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac
    25801 ttaacgggcg gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt
    25861 tattttgtgg gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat
    25921 caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca
    25981 tcaccttgtg accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt
    26041 acgaggattt atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga
    26101 tctgtacata atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag
    26161 ccagacgaac gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt
    26221 gtggaaaaag atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg
    26281 atttactaat gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa
    26341 gcaccgtttt gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaaaaaga
    26401 tcaaaaaaat acttgtgcaa aaaattggga tccctataat gcgcctccgt tgagacgaca
    26461 acgtgaaaca cttcacaaga tggtcgtaac aacgaagaga aaaaatcctg aaattcaggg
    26521 ttgactctga aagaggaaag cgtaatatac gccacctcgc
//
LOCUS       DAPHJE010000061        26493 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPHJE010000061 DAPHJE010000000
VERSION     DAPHJE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.061"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            207..1151
                     /locus_tag="RH222_002582"
     CDS             207..1151
                     /locus_tag="RH222_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3797584.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            1337..2719
                     /locus_tag="RH222_002583"
     CDS             1337..2719
                     /locus_tag="RH222_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3797585.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATPPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            2756..3478
                     /gene="folM"
                     /locus_tag="RH222_002584"
     CDS             2756..3478
                     /gene="folM"
                     /locus_tag="RH222_002584"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV3797586.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(3475..3810)
                     /locus_tag="RH222_002585"
     CDS             complement(3475..3810)
                     /locus_tag="RH222_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV3797587.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            3939..4658
                     /gene="rstA"
                     /locus_tag="RH222_002586"
     CDS             3939..4658
                     /gene="rstA"
                     /locus_tag="RH222_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV3797588.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            4662..5963
                     /gene="rstB"
                     /locus_tag="RH222_002587"
     CDS             4662..5963
                     /gene="rstB"
                     /locus_tag="RH222_002587"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV3797589.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRSQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            6039..6968
                     /gene="tus"
                     /locus_tag="RH222_002588"
     CDS             6039..6968
                     /gene="tus"
                     /locus_tag="RH222_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV3797590.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHNLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(6965..8368)
                     /gene="fumC"
                     /locus_tag="RH222_002589"
     CDS             complement(6965..8368)
                     /gene="fumC"
                     /locus_tag="RH222_002589"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV3797591.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(8511..10157)
                     /gene="fumB"
                     /locus_tag="RH222_002590"
     CDS             complement(8511..10157)
                     /gene="fumB"
                     /locus_tag="RH222_002590"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV3797592.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10356..11531
                     /gene="manA"
                     /locus_tag="RH222_002591"
     CDS             10356..11531
                     /gene="manA"
                     /locus_tag="RH222_002591"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV3797593.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFSIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCQGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            11632..13140
                     /locus_tag="RH222_002592"
     CDS             11632..13140
                     /locus_tag="RH222_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV3797594.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(13185..14450)
                     /gene="uidC"
                     /locus_tag="RH222_002593"
     CDS             complement(13185..14450)
                     /gene="uidC"
                     /locus_tag="RH222_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV3797595.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
     gene            complement(14489..15862)
                     /gene="uidB"
                     /locus_tag="RH222_002594"
     CDS             complement(14489..15862)
                     /gene="uidB"
                     /locus_tag="RH222_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV3797596.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDITN"
     gene            complement(15859..17670)
                     /gene="uidA"
                     /locus_tag="RH222_002595"
     CDS             complement(15859..17670)
                     /gene="uidA"
                     /locus_tag="RH222_002595"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV3797597.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAANKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(18059..18649)
                     /gene="uidR"
                     /locus_tag="RH222_002596"
     CDS             complement(18059..18649)
                     /gene="uidR"
                     /locus_tag="RH222_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV3797598.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(18878..19645)
                     /gene="hdhA"
                     /locus_tag="RH222_002597"
     CDS             complement(18878..19645)
                     /gene="hdhA"
                     /locus_tag="RH222_002597"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV3797599.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(19757..20785)
                     /gene="malI"
                     /locus_tag="RH222_002598"
     CDS             complement(19757..20785)
                     /gene="malI"
                     /locus_tag="RH222_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV3797600.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20960..22552
                     /gene="malX"
                     /locus_tag="RH222_002599"
     CDS             20960..22552
                     /gene="malX"
                     /locus_tag="RH222_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV3797601.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLNC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            22562..23734
                     /gene="malY"
                     /locus_tag="RH222_002600"
     CDS             22562..23734
                     /gene="malY"
                     /locus_tag="RH222_002600"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV3797602.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            23838..24839
                     /gene="add"
                     /locus_tag="RH222_002601"
     CDS             23838..24839
                     /gene="add"
                     /locus_tag="RH222_002601"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV3797603.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLAKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(24874..25914)
                     /locus_tag="RH222_002602"
     CDS             complement(24874..25914)
                     /locus_tag="RH222_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3797604.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            26157..26282
                     /gene="blr"
                     /locus_tag="RH222_002603"
     CDS             26157..26282
                     /gene="blr"
                     /locus_tag="RH222_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV3797605.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
BASE COUNT         6472 a         6840 c         6461 g         6720 t
ORIGIN      
        1 tacaattcag ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg
       61 tcatttgagc aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt
      121 tacaccgttt aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacttgg
      181 tttgcgcaag gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac
      241 tgctttctgc tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg
      301 cagcggttaa accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag
      361 cggtgaaagc cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg
      421 acacttctga ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg
      481 atgctgaaaa agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga
      541 aagatcaggc tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta
      601 gccagccaga agtcaatgat gccatcacca aagcagcaaa agcgaaaggt gcctactctt
      661 tctacatcgt tcgtcaaatc gatgccaacc agggcggcaa ccagcgtatt actgcattca
      721 tctataaaaa agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt
      781 ccgaagcagg acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc
      841 cgggtgttgc gactaccgca tcaccaagtt ctgaagtcgg tcgcttcttt gaaacccagt
      901 catcaaaagg cgggcgttac accgtcacgc tcccggatgg cactaaagtc gaagaactga
      961 acaaagcgac cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg
     1021 gcaacatgac cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt
     1081 attaccacat cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc
     1141 tgtataaata actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt
     1201 agtcacaaat ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct
     1261 tcagtggcaa actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt
     1321 taatcactgg atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt
     1381 aagctcaatg ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag
     1441 cccggcagca ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt
     1501 tgccatgctg atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc
     1561 ccgcgaaggg tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc
     1621 agtcatcgcc aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga
     1681 cacgccggaa ttgcgcctgt tcggcgatgg taatacctgg caatcgatag tcggtgcatc
     1741 ggctttattg tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat
     1801 taacctggtg gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat
     1861 gatgatgttc aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc
     1921 cgtgtgggaa caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga
     1981 aggtgccgtg gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact
     2041 gctggcggtt ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggcgt
     2101 ggtggcacgt cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga
     2161 aatgatggga ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg
     2221 cgcgtactta agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa
     2281 agcattccca cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcgctgtg
     2341 gttgaccaac atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta
     2401 caacacgttg ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc
     2461 gttcttgctg aaaatcgcaa cccctccact gcataaagcg gtaggtgtcg gtgcctgcat
     2521 ttatggctta tggttattgt atgcctctgg tccaatgcac ttgttgttat ccgttgttct
     2581 gtatgcgccg gggctgctgg ttttcctgta tgcgcgtaaa acgcatacac atgataacgt
     2641 actgaaccgc caggaaatgg tgctaattgg catgctgtta attgcctcag ttccggcgac
     2701 ctggatgctg gtgggataac gttcatccca tcgttggtta gcaaaggaga taacgatggg
     2761 taaagcccag cccttgccaa tattaattac tggcggtggt cgtcgcatcg gcctcgccct
     2821 cgcatggcat ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cacactatcc
     2881 agccattgat ggactgatta atgcaggtgc gcagtgtatt caggctgatt tttcgaccaa
     2941 cgacggtgtg atggcgtttg ccgatgaagt actaaaaagc acccatggtc tgcgtgctat
     3001 tttgcataac gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact
     3061 ggcttgcatg atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag
     3121 attactgcgt gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt
     3181 ggagcgcggt agcgacaaac atgttgcgta tgctgcaagc aaagcggcac tggataatat
     3241 gacccgctcg tttgcccgca agctggcacc ggaagtgaaa gtgaattcta ttgcgccatc
     3301 gctgatcctg tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc
     3361 actgatgaaa accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac
     3421 cagttgcttt gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg
     3481 cagctttatc cagcatataa tcaacaccca tgcgcttatc ccccaacacg caacagaacc
     3541 aacaaaagcg gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac
     3601 aaaataggga ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc
     3661 gcgttcgctg gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag
     3721 cccggcgata taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc
     3781 accgagcgcg gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc
     3841 atcagtagaa tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg
     3901 cggtgtattg tgacgttttt atatctaccg tgaataatat gaacactatc gtatttgtgg
     3961 aagatgatgc ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg
     4021 ttaccgtaga gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt
     4081 tggtgttact cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac
     4141 gcgcaaagtg gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca
     4201 tcctggcact ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt
     4261 tgctagcgcg tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc
     4321 ttcaggaaac gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc
     4381 ccatcaaccg cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg
     4441 aattattgtg ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga
     4501 aaaatttacg cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc
     4561 ggttaagaaa aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta
     4621 acaaaggcta tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc
     4681 agttttacct gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt
     4741 acaaatttac cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcgc
     4801 tgtatctgat gcgtagcgaa ttgcgtgaga tccccccaca cgattggggt aagacgctga
     4861 aggagatgga tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc
     4921 ttgatgatat ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt
     4981 acacgttttt gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt
     5041 atctttatta tctccatcag atgcgattgc tggatatcgc cctgatcgct tttattgcta
     5101 tttccctcgc ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac
     5161 tggaagcagc ggcgcaacga tttggcgatg ggcatctcaa tgagcgtatc cactttgatg
     5221 agggttcgag ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg
     5281 ccttgattgc cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt
     5341 tagtgcgcct gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg
     5401 cgttgaatcg tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc
     5461 gactcgatcg ctcacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt
     5521 caacgcatct ggcagatatt caggcagtaa cgcccgataa aacggtacgg ataaaaacgc
     5581 tcgtgcaagg ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt
     5641 tgctcaataa cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg
     5701 ggaatagagc gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg
     5761 aacatatctt tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct
     5821 gcgggctggg gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt
     5881 gtgacaccag cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca
     5941 tcccgcaatt tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accattgtgc
     6001 tataaattaa ggatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag
     6061 accgactcaa cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc
     6121 ttgagcaaca caatctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg
     6181 atgagcataa tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt
     6241 cgctggcgtt gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca
     6301 gcaaggccgc tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag
     6361 cagcgttggt cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg
     6421 tcacggttga atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc
     6481 cggggctgat cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt
     6541 tacgctttgg ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg
     6601 cacagctgga aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt
     6661 ggcaaagaaa actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt
     6721 taaaaatcaa acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc
     6781 aaaaacaagt gcaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg
     6841 gtgcgggcgt gccagacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc
     6901 gttataaacc acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg
     6961 cagattaacg cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa
     7021 actcggcttc gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt
     7081 tatgcgcttt tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag
     7141 tcaccagcat cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac
     7201 ccactgcgca gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca
     7261 ggaaattatg gatcaccatc ggacggaaga cgttcagttc aaagttaccg gaagcgccac
     7321 ccatgttgat cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac
     7381 actgggttgg gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg
     7441 agatttcacc aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt
     7501 tcatcagtga cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg
     7561 tcgcaagcgc ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca
     7621 gttcatctgc tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg
     7681 taccgccaag agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat
     7741 gctcgagcat cgctacccag ccggaaatct cctgccctag tgttagcggc gtggcgtcct
     7801 gcaagtgggt tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg
     7861 tctgtgtcag ggttttaagc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa
     7921 cgtgcatcgc cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag
     7981 ggtgaacttt acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca
     8041 gcacttcgtt catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg
     8101 ggaattcgtc gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg
     8161 ctttctcttc agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta
     8221 gcgccagcgc atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca
     8281 gcgagcgttg agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg
     8341 aatctttttc gctgcgtact gtattcatgg cctgctcctc acctgtttaa ttttttcttt
     8401 ctgttttgct ttcgttaagc aacttttagc tcactcatta tttactattt gataacaaat
     8461 gtttggtctt tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca
     8521 cagcgggtgc attgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca
     8581 aggataaacg ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc
     8641 ggatattcaa cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca
     8701 ccgatactgc cgaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg
     8761 cggttgcctt tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa
     8821 gaatccatcc gtccggcggt cgttgggcca agagaaccgg aagcataacc ttccggcgtt
     8881 ttggccggac ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcacca
     8941 ttatccatcc gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg
     9001 ttaagcgata agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc
     9061 ggacggttaa ggtcaacgcg caccgcttcg ccttctcccg ctttgcgcag ctcttccggg
     9121 atatatttgc ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc
     9181 gctttgatat tacggtcagc agagcaggag acgcccatac cgaccgggca ggatgcgccg
     9241 tgacgtggca ggcgaatcac gcggatgtcg tgagcgaagt atttaccacc aaactgcgca
     9301 cccagaccga gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat
     9361 gcctgaccgt gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt
     9421 ttcactgttt taaggttcgt ttctgcagaa gttccaccaa taacgaacgc aatatgatac
     9481 ggaggacagg ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc
     9541 cccggcgtta gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccgcca
     9601 cctttggcga tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc
     9661 gctggtagat tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag
     9721 tagcgcagat tatcttcgat ataagtgtta tagacaccgc gtgccagcgc cgcttcatca
     9781 ccgccaccgg tccatacacg ctgccctttt ttaccaacaa taatcgcggt gccggtatcc
     9841 tgacaggttg gcagaacgcc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc
     9901 acatatttat cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg
     9961 tgagccggac gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac
    10021 gcttcgggtg cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg
    10081 ctggttagca ggtaatactc agtatcatct tttttgagtg ggaaaggagc ctgataatga
    10141 aagggtttgt ttgacattat tctctcactt actgcctggt ttggttatgc tctgggcggg
    10201 tgttccgttg ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact
    10261 gagactagta cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag
    10321 cgggattaat ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc
    10381 aaaactatgc ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt
    10441 ccagccagcc gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc
    10501 agaatgccgc cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc
    10561 tgctcggaga ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat
    10621 gcgcagcaca gccgctctcc attcaggttc atccaaacaa acacaattct gaaatcggtt
    10681 ttgccaaaga aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc
    10741 ctaaccacaa gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc
    10801 gtgaattttc cgagattgtc tccctactcc agccggtcgc aggtgcacat ccggcgattg
    10861 ctcacttttt acaacagcca aatgctgaac gtttaagcga actgttcgcc agcctgttga
    10921 atatgcaggg tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcc ctcgatagcc
    10981 agcagggtga accgtggcaa acgattcgtt taatttctga attttacccg gaagacagcg
    11041 gtctgttctc cccgctattg ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc
    11101 tgttcgctga aacaccgcac gcttacctgc aaggcgtggc gcttgaagtg atggcgaact
    11161 ccgataacgt gctgcgtgcg ggtctgacgc ctaaatacat tgatattccg gaactggttg
    11221 ccaatgtgaa gttcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag
    11281 gtgcagaact ggacttctcg attcctgtgg atgattttgc cttctcgctg catgatctta
    11341 gtgataaaga aaccaccatt agccagcaaa gtgccgccat tttgttctgc gtcgaaggcg
    11401 atgcaacgct gtgtcaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta
    11461 ttgccgccaa cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca
    11521 acaagctgta agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta
    11581 taacaccttc aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa
    11641 tcgctggtag cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg
    11701 tatacaggca agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa
    11761 ctcaaactga cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc
    11821 gtattcagca gccagttgca actgttggtg aaacccattg ctgggaaaga aaatccgtgg
    11881 attaaaagcg gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttccccctt
    11941 gcccagctta aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtc
    12001 aataacgaag taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt
    12061 aactcccgca ttggttacag cggtgattcc agttccgata tttcgctcaa tccactgaat
    12121 tacgagcaaa aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac
    12181 agagacggca aagctatctc cctttccggg gaagcgcaaa gtggtcggat agacgcagtt
    12241 aacgaataca atcagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc
    12301 acgctggcaa gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc
    12361 atttcagtgg aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg
    12421 gatctggtca atgatggtaa aacgatcaat agccaactgg attactcgct aaacagcctg
    12481 aaggtacaga atcaggatct gggcagcggc aagctgactt taaaagtcgg ccaaattgat
    12541 ggcgaagcct ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca
    12601 cagccagaaa ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt
    12661 agcgccctgc cgctgatgct gaaaggcgat ccggtgatta ctattgcgcc gctaagctgg
    12721 aaaaacagtc agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg
    12781 actaaagaag cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat
    12841 gcgaaactga ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg
    12901 gaaggttatc aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca
    12961 gcaatggggc agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg
    13021 cagtatgcta acggtcagat cacgttaaac gggcagaaaa tgccactgga agattttgtt
    13081 gggatgtttg cgatgccggc attaaacgtt ccggctgtac ccgctattcc gcagcagtaa
    13141 ttcacagcca aaacgccgga ggtttttctc tccggcgtta tttcttactt cagcataaag
    13201 tcatactgaa tactcatttt cacatcccag taattcaggc gatcgcccag cgcactgcca
    13261 tcgcgctttt cccgcccttt atcagcagca agaaagaaat tcatcccttg taacggccct
    13321 tcgctgaccc gatagttcat accacctaag atacccgtgc cttcaatccg cacatctcgt
    13381 ttactggatt catagccgcg agtcacaatc ggtgcaaatg tcagtgtaaa ttgcggcgtt
    13441 aaacgatagt tgacgcccaa ttgccaggac tgcattcctt cacggttacg atcaagactt
    13501 aatgaaaagg gatagccaat gtcggtatcc accacggcac cattcattgc atggcgcaaa
    13561 taaccaccac tgccaaatac gcttccccac ggcgcattcc aggtcaattg tccactaacc
    13621 aacgccgttt cattgggctg gctggtatta cgacttaaac cttcgagctg cgcgtaaaag
    13681 ccgagcaact cccctttcaa cgtataattc gggtcgagcg gtatgctttt ctcgagaatg
    13741 ctttgtatct gattggaaac atctttctgt accgatgccc ccagagaata gcgactggta
    13801 tcatcatgcc aactggcggc taaaaaagaa cgtgcgcgat gtttatcgta ttttcccgtc
    13861 tggctgttat acaccccttc atcacgaata ccggttgttg aatcggtcca taaaaatact
    13921 cgtgaacgcc acattcccca cagatcaata tttggcgtta acgcccaata acctaatgct
    13981 ccttcagaaa ctgtcggtaa tgtacgttgc gaactgccag ccattaacgg aatacgataa
    14041 ggtaagctac cgtaactata atccgtcccg aagcgaccta atttaagcag gctattttca
    14101 ctgggtttta tttttactgc ccccagggca aagccaaaac ttttatcacc atcaagatac
    14161 caccgggtac tcatatcagc acgagcacct aatttataaa cataatacgc tccgccttca
    14221 acgccaataa tattggaata ataaccacta ttgaaatcga gcaatcctcc ctgtacccat
    14281 gcgtaaatat ctcttcccgc gccagcactg ggcttatccg ccctgcgcaa ttcgtttttc
    14341 agtctgatgc gtagtcccgc ttcatcgtca gccagttgcg ccgcgtaagc agaggtaagg
    14401 ccagaggcag ccgtcaggca aataacggcc atggccacta tttttctcat agttgcacct
    14461 ttgatgttct gattattttt attgaatatt aattagtgat atcgctgatt aattgctgct
    14521 gcattttttt acgattatca atttcaacca cgatttcttt gaatttttta tccgtgagcg
    14581 gataaaacca gataataacg aatgccagta gcataaatcc gcaaggtact aaggcaattg
    14641 atgtgcggat gcccataata acttccggcg tttgcacctg attggcgata tatccgctta
    14701 atcccaaaat aaaggcagga attgaacctc cgattgcctg accgcattta cgggtaaatg
    14761 agaatagtga ataggtgagc ccttcaattc gcacgccggt caggtattca ccgtattcta
    14821 cggtatcagc ttccagtgcc cacatcacag tcatagtaac gccctgacca attgaagcga
    14881 tggccaacgc aaccaacgcc accggcagtg accagacgga aacccagaag aatagcagat
    14941 aaccgcaggt tcccagcaaa gcgccaatca ggaaggtatt ctttttaccg atcctcgcga
    15001 ccatccccgg caccagcggt gccgatgcca cagtaccgac caggttttgc accagtacca
    15061 gcacagtaaa cagcccggta tcatttaaca catagcgcac gtagaacaac gacgaggcgc
    15121 taaccgcgaa ggtcgaaatc agcacacaca gcgcaccgat gcacaacata aacagcgggc
    15181 gattccgttt cagggtttgc agactgatct tcaatgacgg ctgcgccacg atacgtacca
    15241 cattctcacg cgtcgatttg aagcagatga agtaaagcac cattccggca atcgccagca
    15301 caatcgtcca gaaatggtat accgacacca tctcttccgg gctggagttc ttaatgctcg
    15361 gtcctatcag aaatgccagg cagacaaagg ttaatgaagc ggcaatcccg cgagccgcgc
    15421 ccagacgggc gcgggattgt ggttgttggg tcatcgcggt agcaagtgaa ccataaggaa
    15481 tattcaccag gctgtagcaa agcccgaggc ccatgtaggt caaatatgca tacaccactt
    15541 tgctaccatg gctccagtcg gtcggcaccc agaataccag cacgctgaag atcattaacg
    15601 gcgcagtacc gaagagtaaa aacgggcgga attttcccca gcgggtattc acactgtcca
    15661 ccactcgtcc ggcaaagacg tcggcgaagg catcgaatac ccgcaccagt aacagcatgg
    15721 tgcccgccgc agcggcaccg acgccagcga cgtcggtgta gtaactcaac aggaagagcg
    15781 cccccattgc gaaggcgaag ttattggcga cgtcaccgag gctgtagccg acgatggtgc
    15841 gccaggagag ttgttgattc attgtttgcc tccctgctgc ggtttttcac cgaagttcat
    15901 gccagtccag cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt cgcgagtgaa
    15961 gatccctttc ttgttacctc caacgcgcaa tatgccttgc gaggtcgcaa aatcggcgaa
    16021 attccatacc tgttcaccga cgacggcgct gacgcgatca aagacgcggt gatacatatc
    16081 cagccatgca cactgatact cttcactcca catgtcggtg tacattgagt gcagcccggc
    16141 taacgtatcc acgccgtatt cggtgatgat aatcggctga tgcagtttct cctgccaggc
    16201 cagaagttct ttttccagta ccttctctgc cgtttccaaa tcgccgcttt ggacatacca
    16261 accgtaataa cggttaaggc acagcacatc aaagagatcg ctgatggtat cggtgtgagc
    16321 gtcgcagaac attacattga cgcaggtgat cggacgcgtc gggtcgagtt tacgcgttgc
    16381 ttccgccagt ggcgcgaaat attctcgtgc accttgcgga cgggtatccg gttcgttggc
    16441 aatactccac atcaccacgc ttgggtggtt tttgtcacgc gctatcagct ctttaatcgc
    16501 ctgtaagtgc gcctgctgag tttccccgtt gactgcctct tcgctgtaca gttctttcgg
    16561 cttgttggcc gcttcgaaac caatgcctaa agagaggtta aagccgacag cagcagtttc
    16621 atcaatcacc acgatgccat gttcatctgc ccagtcgagc atctcttcag cgtaagggta
    16681 atgcgaggta cggtaggagt tggccccaat ccagtccatt aatgcgtggt cgtgcaccat
    16741 cagcacgtta tcgaatcctt tgccacgcaa gtcagcatct tcatgacgac caaagccagt
    16801 aaagtagaac ggtttgtggt taatcaggaa ctgttcgccc ttcactgcca ctgaccggat
    16861 gccgacgcga agcgggtaga tatcacactc tgtctggctt ttggctgtga cgcacagttc
    16921 atagagataa ccttcacccg gttgccagag gtgtggattc accacttgca aagtcccgct
    16981 agtgccttgt ccagttgcaa ccacctgttg atccgcatca cgcagttcaa cgctgacatc
    17041 accattggcc accacctgcc agtcaacaga cgcgtggtta cagtcttgcg cgacatgcgt
    17101 caccacggtg atatcgtcca cccaggtgtt cggcgtggtg tagagcatta cgctgcgatg
    17161 gatcccggca taattaaaga aatcatggaa gtaagactgc tttttcttgc cgttttcgtc
    17221 ggtaatcacc attcccggcg ggatagtctg ccagttcagt tcgttgttca cacaaacggt
    17281 gatacgcaca cttttcccgg caataacata cggcgtgaca tcggcttcaa acggtgtata
    17341 gccgccctga tgctccatca cttcctgatt atttacccac actttgccgt aatgagtgac
    17401 cgcatcgaaa cgcagcacga tacgctggcc tgcccaacct ttcggtataa agacttcgcg
    17461 ctgataccag acgttgccca cataattacg aatatctgca tcggcgaact gatcgttaaa
    17521 actgcctggc acagcaattg cccggctttc ttgtaacgcg ctttcccacc aacgctgatc
    17581 aattccgcag ttttcgcgat ccagactgaa tgcccacagg ccgtcgagtt ttttgatttc
    17641 acgggttggg gtttctacag gacgtaacat aagggactcc tcattaagat aataatactg
    17701 gtcaaccttt aatctgatta gttaaccaat tctgtgttgc acacagttat ctgtgagaaa
    17761 cccatggaag agaaatggaa ataaaaagaa ctgacaccgc gatcaaaaaa ccaaaaactg
    17821 caatacccac agcgtgatat ggatcgcatt aatctttaaa acagcgttga cagaagccat
    17881 aaaataagca atcgaacgtt cgttcgagta tcttgagaac gataaactct catgacatat
    17941 ggctacggtg aatattttgg ggggggaatt ctccgatccc ggggagattg tattttgtac
    18001 agggaaatta aagtatgaaa cgcgggcaca gttatcacca gcccgcgaaa tgagaggatc
    18061 aggatgcggt taagataccg ccaatcatcg cgcgtaatcc ctgagcaaac gacgcttcac
    18121 gcgccagatc ttccgcttcg atatcggcca gaacaccgta ggtcagatcc agcagtaaac
    18181 gtgaagtcat tgccgtgttg atgtctgggc ttatctcgcc tttttgctgg gcatcacgca
    18241 tccgctgagc aacaaattca gtgatcgtca tatgcttatt tttaagcatg gcggcgacct
    18301 gtgggttacg catcccttcc gccataattt cgaccaccag cgcccgttgc ccaaaggctt
    18361 catgggtgag agagacaata gactcgacca tatagtcaac aaaatgaatc ccttcaatcg
    18421 gttcccggaa acgggccagc gccctctcct ggtcctgtaa gataatcgcc tgaatcaagg
    18481 cttctttgga gatgaaatgg tgatagagcg tcccgggact aatggcgcaa gatttacaga
    18541 tggctttcat cgaggcactg tgaaatccat tttctgaaaa aatctctctg gcagcattga
    18601 ggatccgggt ccgtgtcggt tgtgcttcag tctgcatgtt atccatcatt aaaccattat
    18661 agttagcgtg aataactagt ttaccgcgtt gtcaggtgag cgcctatcat tgctgttgaa
    18721 tagtgcgaag gcacactcta ttcgtatgct tttcatccag ctattggtta accaatagcc
    18781 gtgatttggc tgccagttgt ttagcagaga cgttgatgaa ttgcacacgc tatcccggta
    18841 aacaacacgt acggctgttt accggtccgt tagtgtatta attgagctcc tgtaccccac
    18901 caccggagac tgtgagaatt tgtccgctta cccagctggc tgcaggcgag caaaggaaca
    18961 gagctgcgtt agcaatatct tgcggttggc ccagacgtct gatcggcgtg tgctgtaaca
    19021 ttttttgttc aatttctggt gtaataacgg atttcagggc atcggttaat attgcccccg
    19081 gcgcaatgcc atttacccga atattttttt cacccaggtc aaacgccata tttctgacca
    19141 gatgactggc cgcagcttta gatgatgcat aggatgtcat gtttatattt ttattttctg
    19201 ccgccataga agtgatggtc agaataacgc caccgccatt tttttccatt tctggcgcaa
    19261 caagttgtga cagatggaaa aaagaaaaca cattcagttc ataagcacgg cgaaaatctg
    19321 ccattggcat atcaaacggt ttaggaccac cgccaccggc gttgttaacc agaatatcaa
    19381 ctttacccag cttactgata gcaaagtctg ccagtgcaga gagttcctgt tcggaagtaa
    19441 tatcacaacg gcaggcaaat gcctgaccac ccagttgttg aatttcgtct acaacatggt
    19501 tagctgcgtc ggcgttaata tcactgacca ccacagatgc gccagctgtc gcgaatgtaa
    19561 tggcgatttc tttaccaata cctgcacccg cacctgtgat gatggcgcat tttccgtcga
    19621 gtctcaggtt gtcagaatta aacacataaa cctcctgtga acttcagtaa aaataaaagg
    19681 caccgcctgt gtgccacatt ttttatcatt aaccttgagg aaaggacact accaggtata
    19741 gcagataaag agaggattat ttcgccgcaa tgagccgggc gggaataata agattgcgtg
    19801 aatgcgtctc ttcatgggtg attttttgca tcatgcgatc cgcaagtgtg gtaccaagtt
    19861 cccgcgctgg cgtgctggcc caggtaacag gtatatcatc aagtgtggtt ggtgtcgcat
    19921 cggtaaatgc cgccagcgaa acctgttgct caaagtaacg atcgactccg ctttccccgc
    19981 tttgccgccc tgctttcagc aaaccaaacc atgcccccat cgcaatagtt tcgttatagc
    20041 aaaccacggc actgatggtc gggttatgac gtaaaagcgc cgtgatagct tccgctgctt
    20101 gcttctggct ggaagtgcac tccaacaccc aatcgctgtg aaacggcagg ccaaatttta
    20161 gtagagttgc acaatagccc cccacccgtt ctgcacgggt taatgaggaa ctttgccctc
    20221 ccagccaggc gatccgctga tgcccattgc gaatgagatg ctccgtcaac aactgtgcag
    20281 cctgcatgtt gtccgggcga accgtatcaa catcatcaag ataactggca cgggaagcga
    20341 aaatcaccgg gatagctttt tcttctgcca tccgtcgcag gtcatcgctg cttcctgcag
    20401 ccccggctat taccacacca tcgacaccct gattcagtaa cagtgaaaac cgctgtgcca
    20461 gctgctcgcc gtctttaccg ccgtgaagca aaaaaaccat ccgtccctgc gcttccagag
    20521 cttccgtcaa tccggccgtc aattcggcgt aaaacggcgc agataaatca cggacgatca
    20581 aaccaatgac gccgctttgt ccgccgcgca gcgccgacgc ctggcgattg cgcacaaatc
    20641 ccagctcttc aatggcggcg ttaacgcgtt ctcctgtggc ggtagagatt cgccctttgc
    20701 cactaagcac cagcgaaacg gtacttaccg acacgcccgc agccagcgca acatcatgaa
    20761 tggttatttt tttggcggta gccatgcaga tgacctactc cctgattatg tgacagataa
    20821 aacgttttac cttttattta tcttataccc gctattatcg ttgcgtaatg tgatttatgc
    20881 ctcactaaaa tttgataaaa cgttttatct tctcgcgcaa tttactgaat ccagattgtt
    20941 ctctacgagg agtcgtttta tgacggcgaa aacagcaccg aaagtcacgc tgtgggagtt
    21001 cttccagcag ttaggcaaaa ccttcatgtt acccgtggca ttattgtcgt tctgcggcat
    21061 tatgctcggc attggcagtt cccttagtag ccacgatgtc atcaccctaa tcccggtcct
    21121 gggcaacccc gtgttgcagg ctatctttac ctggatgagt aagattggct cgtttgcttt
    21181 tagtttcctg cctgtcatgt tctgtatcgc catcccgctg ggcctggcac gcgaaaacaa
    21241 aggcgtagcg gcattcgctg gcttcgtcgg ttatgcggta atgaacctcg cggtaaactt
    21301 ctggttgacc aataaaggca ttctgccaac cacggatgcc gcggttctga aagccaataa
    21361 catccagagc attcttggga tccagtcgat cgataccggg atcctcggtg cggtgatcgc
    21421 cggtattatc gtctggatgc tgcatgagcg tttccataat atccgcctgc cggatgcgct
    21481 ggcattcttc ggcggtacgc gcttcgtacc aattatctcc tcgctggtga tgggccttgt
    21541 cggcctggtg attccattag tctggccgat tttcgccatg ggtattagcg gcttgggcca
    21601 tatgataaac agcgcgggtg atttcggacc gatgctgttt ggtaccggtg aacgtctgct
    21661 gttgccgttt ggtctgcatc acattctggt ggcattaatt cgctttaccg acgcaggcgg
    21721 cacgcaggaa gtctgcggtc aaaccgtcag cggcgcactg accatcttcc aggcgcaatt
    21781 gaattgcccg accactcacg gtttttctga aagcgccacg cgtttccttt cgcaaggtaa
    21841 aatgcctgcg tttctcggcg gtctgccagg tgcagcgtta gctatgtatc actgcgcgcg
    21901 cccggaaaat cgccataaaa ttaaaggtct gctgatttct ggcctgatcg cctgcgtcgt
    21961 tggcggcact accgaaccgc tggaattcct gttcctgttc gtagcgccag ttctgtatgt
    22021 catccacgcg ctgttaaccg gcctcggctt caccgtcatg tctgtgctcg gcgtcaccat
    22081 cggtaatacc gacggcaata tcatcgactt cgtggtgttc ggtattttgc atggtctgtc
    22141 aaccaagtgg tacatggtgc cagtggtggc ggcaatctgg tttgtcgttt actacgtcat
    22201 cttccgtttc gctatcaccc gcttcaatct gaaaaccccg gggcgcgata gcgaagttgc
    22261 cagctcaatc gaaaaagccg ttgccggtgc gccgggtaaa tcaggttaca acgttcctgc
    22321 aatcctcgaa gcattaggcg gtgccgacaa tattgtcagc ctcgataact gcattacccg
    22381 tctgcgtttg tctgtgaaag atatgtcgct tgttaatgtg caggcactga aggacaatcg
    22441 ggcaattggc gtagtacaac ttaatcaaca taacctgcag gttgttatcg ggccacaagt
    22501 tcagtcagta aaagatgaaa tggccggtct gatgcatact gtccaggcat aaggatacga
    22561 tatgttcgat ttttcaaagg tcgtggatcg tcatggcaca tggtgcacgc agtgggatta
    22621 tgtcgctgac cgtttcggca ctgctgacct gttaccgttc acgatttcag acatggattt
    22681 tgccactgcc ccctgcatta tcgaggcgct gaatcagcgc ctgatgcacg gcgtatttgg
    22741 ctacagccgc tggaaaaacg atgagtttct cgcggctatt gcccactggt tttccaccca
    22801 gcattacacc gcaattgata cgcagacggt ggtgtatggc ccttctgtca tctatatggt
    22861 ttcagaactg attcgtcagt ggtctgaaac aggtgaaggc gtggtgatcc acacgcccgc
    22921 ctatgacgca ttttacaagg ccattgaagg taaccagcgc acagtaatgc ccgttgcttt
    22981 agagaagcag gctgacggct ggttttgcga tatgggcaag ctggaagccg tgttggcgaa
    23041 accagaatgt aaaattatgc tcctgtgtag cccacagaat cctaccggta aagtgtggac
    23101 gtgcgatgag ctggagatca tggctgacct atgcgagcgt catggtgtgc gggttatttc
    23161 cgatgaaatc catatggata tggtctgggg cgagcagccg catattccct ggagtaatgt
    23221 ggcgcgggga gactgggcgt tgctgacgtc gggatcgaaa agtttcaata ttcccgcgct
    23281 gaccggtgct tatgggatta tagaaaatag cagcagccgt gatgcctatt tatcggcact
    23341 gaaaggccgt gatgggcttt cttccccttc ggtactggcg ttaactgccc atatcgccgc
    23401 ctatcagcaa ggcgcgccgt ggctggatgc cttacgcatc tatctgaaag ataacctgac
    23461 gtatatcgca gataaaatga acgccgcatt tcctgaactc aactggcaga tcccgcaatc
    23521 cacttatctg gcatggcttg atttacgtcc gttgaatatt gacgacaacg cgttgcaaaa
    23581 agcacttatc gaacaagaaa aagtcgcgat catgccgggg tatacctacg gtgaagaagg
    23641 tcgtggtttt gtccgtctca atgccggctg cccacgttcg aaactggaaa aaggtgtggc
    23701 tggattaatt aacgccatcc gcgctgttcg ttaaccccaa ttgcgcaacg taaaaaatcg
    23761 ttgcgcaatc gtggattttt accctgcttt gtttttataa tggtgcgcac ttttatatcc
    23821 agaaaaagag tgcgaccatg attgatacca ccctgccatt aactgatatc catcgccacc
    23881 ttgatggcaa cattcgtccc cagaccattc ttgaacttgg ccgccagtat aatatctcgc
    23941 ttcctgcaca atccctggaa acactgattc cccacgttca ggtcattgcc aacgaacccg
    24001 atctggtgag ctttctggcc aaacttgact ggggcgttaa agttctcgcc tctcttgatg
    24061 cctgtcgccg cgtggcattt gaaaacattg aagatgcagc ccgtcacggc ctgcactatg
    24121 tcgagctgcg tttttcacca ggctacatgg caatggcaca tcagctgcct gtagcgggtg
    24181 ttgtcgaagc ggtgatcgat ggcgtacgtg aaggttgccg cacctttggt gtgcaggcga
    24241 agcttatcgg cattatgagc cggaccttcg gcgaagccgc ctgtcagcaa gagctggagg
    24301 cctttttagc ccaccgtgac cagattaccg cacttgattt agccggtgat gaacttggtt
    24361 tcccgggaag tctgttcctt tctcacttca accgcgcgcg tgatgcgggc tggcatatta
    24421 ccgtccatgc aggcgaagct gccgggccgg aaagcatctg gcaggcgatt cgtgaactgg
    24481 gtgcggagcg tattggacat ggcgtaaaag ccattgaaga tcgggcgctg atggattttc
    24541 tcgccgagca acaaattggt attgaatcct gtctgacctc caatattcag accagcaccg
    24601 tagcagagct ggctgcacat ccgctgaaaa cgttccttga gcatggcatt cgtgccagca
    24661 ttaacactga cgatcccggc gtacagggag tggatatcat tcacgaatat accgttgccg
    24721 cgccagctgc tgggttatcc cgcgagcaaa tccgccaggc acagattaat ggtctggaaa
    24781 tggctttcct cagcgctgag gaaaaacgcg cactgcgaga aaaagtcgcc gcgaagtaac
    24841 aaaatggatg gtgcaaattg caccatccat ttttcatgca aggcacaaag tcgcgcgatg
    24901 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
    24961 aaccggattt tcaccatcgc catttaacgc atcacgaata gccgcatagt aagccggata
    25021 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
    25081 tacgccatca cgcatatcgt agccccagtc ttcctgcggt agacgctcgc catttttcag
    25141 acgttcttcc tgtggatcga ggccatattt cacataactg cctcgggatc catgcacgat
    25201 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
    25261 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
    25321 atctaccgtc atgctgaccg gtaaaccaaa aagcgtaatg gcctgatcaa gaagatgtgg
    25381 tgctaagtcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
    25441 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
    25501 cgcgagtaaa ccttttagcg tcaagaaatc gctatcccag cgacggttat ggaatacaga
    25561 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
    25621 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
    25681 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
    25741 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
    25801 gctgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
    25861 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
    25921 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
    25981 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaatag cgaattttcc
    26041 aacgacaaaa gctaaatatc gcaaaaacct cagtaaaaat cttgctggag ctattattgc
    26101 taagtaacat ttaccccctg aagttaatgg atcaatcaag agagatgtgg gctgtaatga
    26161 atcgtcttat tgaattaaca ggttggatcg ttcttgtcgt ttcagtcatt cttcttggcg
    26221 tggcgagtca cattgacaac tatcagccac ctgaacagag tgcttcggta caacacaagt
    26281 aagctctgca cttgtggagc gacatgctgc ccgtccgggt gcatgttttc acttgtcgga
    26341 tattaaacca ggaatttatt atcttgttcg atgttgttgg tgattgtcag ggatagtaaa
    26401 gcgcgttact cttttcagag cagtttttca tccctgaata tttgccatca gcgcattctt
    26461 attattgtaa taacacaagc gttcactttt ggt
//
LOCUS       DAPHJE010000062        25459 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPHJE010000062 DAPHJE010000000
VERSION     DAPHJE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.062"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            180..599
                     /locus_tag="RH222_002604"
     CDS             180..599
                     /locus_tag="RH222_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV3797606.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            599..1867
                     /gene="umuC"
                     /locus_tag="RH222_002605"
     CDS             599..1867
                     /gene="umuC"
                     /locus_tag="RH222_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV3797607.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALSVDEVWGIGRRNSKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1913..2443)
                     /gene="dsbB"
                     /locus_tag="RH222_002606"
     CDS             complement(1913..2443)
                     /gene="dsbB"
                     /locus_tag="RH222_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV3797608.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2589..4130)
                     /gene="nhaB"
                     /locus_tag="RH222_002607"
     CDS             complement(2589..4130)
                     /gene="nhaB"
                     /locus_tag="RH222_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV3797609.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4352..5071
                     /gene="fadR"
                     /locus_tag="RH222_002608"
     CDS             4352..5071
                     /gene="fadR"
                     /locus_tag="RH222_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV3797610.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5123..6655)
                     /locus_tag="RH222_002609"
     CDS             complement(5123..6655)
                     /locus_tag="RH222_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV3797611.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            6985..8283
                     /gene="dadA"
                     /locus_tag="RH222_002610"
     CDS             6985..8283
                     /gene="dadA"
                     /locus_tag="RH222_002610"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV3797612.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GLPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8293..9363
                     /gene="dadX"
                     /locus_tag="RH222_002611"
     CDS             8293..9363
                     /gene="dadX"
                     /locus_tag="RH222_002611"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV3797613.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9749..11485)
                     /locus_tag="RH222_002612"
     CDS             complement(9749..11485)
                     /locus_tag="RH222_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV3797614.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11580..12494)
                     /gene="ldcA"
                     /locus_tag="RH222_002613"
     CDS             complement(11580..12494)
                     /gene="ldcA"
                     /locus_tag="RH222_002613"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV3797615.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12594..13205
                     /gene="emtA"
                     /locus_tag="RH222_002614"
     CDS             12594..13205
                     /gene="emtA"
                     /locus_tag="RH222_002614"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV3797616.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13207..13941)
                     /locus_tag="RH222_002615"
     CDS             complement(13207..13941)
                     /locus_tag="RH222_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV3797617.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14142..14396
                     /locus_tag="RH222_002616"
     CDS             14142..14396
                     /locus_tag="RH222_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV3797618.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14574..15014
                     /locus_tag="RH222_002617"
     CDS             14574..15014
                     /locus_tag="RH222_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797619.1"
                     /translation="MIQKINILDISQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15093..16790)
                     /gene="treA"
                     /locus_tag="RH222_002618"
     CDS             complement(15093..16790)
                     /gene="treA"
                     /locus_tag="RH222_002618"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV3797620.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSYKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17110..18528)
                     /gene="dhaM"
                     /locus_tag="RH222_002619"
     CDS             complement(17110..18528)
                     /gene="dhaM"
                     /locus_tag="RH222_002619"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV3797621.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLLSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELEIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            complement(18539..19171)
                     /gene="dhaL"
                     /locus_tag="RH222_002620"
     CDS             complement(18539..19171)
                     /gene="dhaL"
                     /locus_tag="RH222_002620"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV3797622.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESGYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19182..20252)
                     /gene="dhaK"
                     /locus_tag="RH222_002621"
     CDS             complement(19182..20252)
                     /gene="dhaK"
                     /locus_tag="RH222_002621"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV3797623.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQEPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQQLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20480..22399
                     /gene="dhaR"
                     /locus_tag="RH222_002622"
     CDS             20480..22399
                     /gene="dhaR"
                     /locus_tag="RH222_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV3797624.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22499..25384)
                     /locus_tag="RH222_002623"
     CDS             complement(22499..25384)
                     /locus_tag="RH222_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HDV3797625.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHSGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTNNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVR
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
BASE COUNT         6274 a         6338 c         6573 g         6274 t
ORIGIN      
        1 gtgatatttt tcatttctgc tggatgcgtc gtcgccagaa ggccacgtga gcacaggata
       61 agagaacgaa aaatcagcag tctatgcagc gacaaatatt gatagcctga atcagtattg
      121 atctgctggc aagaacagac tactgtatat aaaaacagta taacttcagg cagattatta
      181 tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc
      241 ttgttcagtg tggctttcct tcaccggccg cagattacgt tgaacagcgc atcgatctga
      301 atcaactgtt gatccagcat cccagcgcga cttatttcgt caaagcaagt ggtgattcta
      361 tgattgatgg tgggattagt gacggtgatt tactgattgt cgatagcgct attaccgcca
      421 gccatggtga tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac
      481 tacgcccgac ggtacagctt attcccatga acagcgcgta ctcgcccatt accatcagta
      541 gtgaagatac gctggatgtc tttggtgtgg tgatccacgt cgtaaaggcg atgcgctgat
      601 gtttgccctc tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga
      661 tttatggggt aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa
      721 cgctgaggca aaggcgcttg gcgttaaaat gggcgatccc tggttcaaac aaaaagatct
      781 gtttcgtcgc tgtggcgtgg tttgctttag cagcaattat gagctttacg cagacatgag
      841 caatcgggtg atgtcgacgc tggaagaatt atcgccccgc gtcgagattt acagtattga
      901 tgaggcattc tgcgatctga caggtgtgcg taattgtcgc gatctgactg attttggcag
      961 agaaattcgc gcaacggtgc tacaacgtac ccatcttact gttggtgtgg ggatcgccca
     1021 gaccaaaacg ctggctaagc ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg
     1081 tggggtggtg gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctctctccgt
     1141 ggatgaagtc tgggggattg gacggcggaa cagcaaaaaa ctggacgcga tggggatcaa
     1201 aaccgttctc gatttggcgg atacagatat ccggtttatc cgtaaacatt ttaatgtcgt
     1261 gctcgaaaga acggtgcgtg aactgcgcgg cgaaccctgt ttgcaactgg aagagtttgc
     1321 accgacgaag caggaaatta tctgttcccg ctcgtttggt gaacgcatca cggattatac
     1381 gtcgatgcgg caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagcga
     1441 gcatcaatat tgtcggttta tctccacatt tattaagacg tcaccatttg cgctcaatga
     1501 accttattac ggcaatagcg cgtcggtaaa actgctgacg cccactcagg acagcaggga
     1561 tatcattaac gccgctacgc gatctctgga tgccatctgg caagcaggcc atcgctacca
     1621 aaaagcgggc gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt
     1681 cgatgacaac gcaccgcgcc ccgggagtga gcaattgatg gcggtaatgg atacactgaa
     1741 tgctaaagag ggcagaggaa cactctattt tgccgggcag gggatccagc aacaatggca
     1801 gatgaagcga gccatgcttt caccacgtta tacaacgcga agttctgatt tactgcgggt
     1861 caaataaaca tagcggcagg aaaaaagcgc tcccgcagga gcgccgaatg gattagcgac
     1921 cgaacaggtc acgttttttc gctttaaacg gctgggaaat caccaccagc accgcgacaa
     1981 tcaggtaagc gataaaaata ccgagcagcc actgcggcat ttccagacct aaaaaatccc
     2041 actgacgctc ggcacaatcg ccagaggcga caaacacttg cggcacccat ttatccagcg
     2101 gcagccattc cgggaaacga accataaaat cacaggtggc aaacggcgaa ggatagagct
     2161 gaagcatggt gtgctcgtat gttaactgca caccgcggaa cgcactatac aaccagataa
     2221 ccatcgctac ataacgcagt ggcgttttcg gggcgatcgc gccaatcagc gcagcaccca
     2281 gaacgccgaa taatgcgcag cgttcataaa tacagagcac gcaaggtttc agtaacatca
     2341 catgctggaa ccacagcgcc gtcagttcca gtgccagagc agtaaacgcc atcaacagcc
     2401 acgcgccccg gccttgtgaa cattggttca aaaatcgcaa cataatcatt tccctgcaat
     2461 atgcatagag tgcgcagttt aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa
     2521 taggacgcaa ttggacaatt atcgggcgaa aaggcaaacc gggcatttat atgcccggta
     2581 agttgttatc aaagcgttgc tatccagccc atttgcataa accattcggt tacaggggca
     2641 agcgtaaact cgacgcagag cagtccgacg agtgtcagta cgagggtgta aggcagggcc
     2701 atccacacca tgcggccata agagaggcga atcaatggcg cgagtgcaga ggtcagcagg
     2761 aacaggaacg cagcctgacc gttcggcgta gcgacggagg gcagattggt accggtatta
     2821 atggcgaccg ccagcagctc gtattgcttc aacgtgatag cgccactttc cattgccgct
     2881 ttcgcttcgt tgatataaat cgtccccacg aagacgttat ccgaaatgga tgacagcaga
     2941 ccgttgaaaa tatagaacag cgacagctga gcatgttccg atgcctgcaa cacaaactga
     3001 ataattggcg aaaacagttg ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc
     3061 aacagtgcgg tgaatggcag agattcggtg aaggctttac cgatagcatg ctcatcggtg
     3121 acaccggtca atgatgttgc cagaataatg actgacaaac caatcaagcc aacttccgcc
     3181 agatgcaacg ccagcgcagt caccagccag acgccaataa tcgcctggac aatcagacgg
     3241 attttatcct gacgggtacg ctggtggcgg ctttgatcgt caaactgttg caacacttcg
     3301 cggactttct ccggcagcgt ttcaccgtag ccaaaccaac gcagcttctc taccagcagg
     3361 caggttaaca ggccacaaat cagaaccgga acggtcaccg gcgacatgcg caggaagaaa
     3421 tcgccaaaat gccagccagc cgctttagcg atgatcaggt tctgtggttc gcccaccatg
     3481 gtcattacgc cgcctaatgc ggtgccgaca ccggcatgca tcatcaggct acgcagaaag
     3541 ccacggaact gttccagaac cactttgtaa tgcttgtcga tatgactatc gtcttgcagg
     3601 tcggtgtctt cggtacggga agaggctacg cgatgataaa taccataaaa accgactgca
     3661 acgctgatca ccaccgccac gacggttaag gcatcgagga acgcggagag gaacgcagcc
     3721 gccacgcaaa aagagagcga cagcagcatt ttggagcgaa tgcttaacag caaacgggta
     3781 aatatgaaca gcaacagctg tttcataaaa tagatacccg ccaccataaa catcagtaac
     3841 agcaagactt caagatttgc cgccacctct tcacggacgt gttccgcgct ggtcatgccg
     3901 atgaataccg cttcgatagc caacagacca ccggggagca gcgggtagca tttcagggcc
     3961 atcgccagag tgaaaataaa ttccgctacc agcaaccagc ccgcgacgaa agggctgatg
     4021 aggaaaatta acggatttac gattaagaaa atgatgaggg cgagtttgta ccagtcgggg
     4081 gactggccca aaaaattgcg ccatagcgcg cggccccagg agatctccat gatggtttcc
     4141 cttaccttac aaataatcaa tgatgttttt atgtttaaac gcaaagctta acggtcaggc
     4201 aggagtgagg caagtcttga tggtcaaggg gaaagagatg cggaaaatga agccttgatc
     4261 ccttttttct tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa
     4321 ctttgttttg ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg
     4381 tttcgcggaa gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat
     4441 tttgcccgca gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga
     4501 agtgttacag cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa
     4561 ggtgaataat ttctgggaaa cttccggttt aaatatcctt gaaacactgg cgcgactgga
     4621 tcacgaaagt gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac
     4681 tatttttatt cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac
     4741 cgctaatgaa gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg
     4801 cggcctggcg tttgcttccg gcaacccgat ttacggtctg attcttaacg ggatgaaagg
     4861 gctgtatacg cgtattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct
     4921 gggcttctac cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga
     4981 aacagtgcgt cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct
     5041 gccgggtgat ttagccattc aggggcgata atcccttccg tttaaagagc aaacccctca
     5101 aacgaggggt tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca
     5161 actcgatgct gccgtcttcg ttttgctgtt cgagcatcac atcaaatccc cacaggcgat
     5221 gcacatgctt cagcacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatgt
     5281 aacgcagcgt cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct
     5341 ccagattact taagttatat tgcgacgata accggttacg gatctcccga taaccttctt
     5401 cattatgaat agcggaaatc tccagataat tatgccgatc atcatccagc acggtgaaga
     5461 agcggaaatc acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga
     5521 aatcacgcat cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc
     5581 agtatttgtc ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga
     5641 acccgagggc atatgggttg atgccgctgt accacgggct gttatagggg ggctggaaga
     5701 ccacattggt gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat
     5761 catacagatg gttaaggatg gtatagtgcc agaaggtcgc ccagccttcg ttcatcacct
     5821 gagtctgttt ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac
     5881 gctgccatga ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg
     5941 gttcggacgg atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg
     6001 tacgccagag catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt
     6061 tctcttcttg cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca
     6121 gcgcgtggca cgagtccaga agccgttcta cttcatcaac gccataacgt tcttcgcact
     6181 cggtaatata tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac
     6241 tgcggaataa gtaattgttt ttaaagaaag agttatgccc atagcaggcg tgtgccatca
     6301 ccagcgcctg catggtaatg gtgttctctt ccatcaggta agcgatacac gggttagagt
     6361 taatgacgat ttcataggcc agtccttgct gaccgtgctt atacagccgt tcagtctcga
     6421 taaacttttt accgaatgac cagtgcggat agttaattgg catgccgacg ctggagtagg
     6481 catccatcat ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt
     6541 agagtttcgc cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt
     6601 cgggtccatc gctcaaacgt gtggtgtcct tattcataga atcgatcgtc gccatacgcg
     6661 cacctcattg ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg
     6721 ttaaaaaccg cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac
     6781 taatccgctt ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta
     6841 aagacgccat attgccgcag agtcagggag atgtgagcca gctcaccata aaaaagccgt
     6901 atgttgaata atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact
     6961 gtatctaccg ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc
     7021 gttgccagcg cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag
     7081 ccgggggccg ccctggaaac cagcgccgcg aatgccgggc aaatctcccc cggatatgct
     7141 gcaccgtggg cggcaccagg tgtgccttta aaagcgatta aatggatgtt ccagcgccat
     7201 gcgccgctgg cggttcgtct cgacggtacg cagttccagt tgaaatggat gtggcaaatg
     7261 ttacgtaact gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg
     7321 gaatacagcc gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg
     7381 cgtcagggtg ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc
     7441 gatatcgccg tgctggaaga tgccggctta ccgtatcagc tgctggaatc cagccgcctg
     7501 gcggaagtgg agcccgcgct ggcagaagta gcgcacaaac tgacgggtgg tctgcaactg
     7561 cccaatgatg aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag
     7621 caggcggggg ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag
     7681 caaatctacg gcgtgaagtg tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg
     7741 tttggttctt actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg
     7801 aaaggctact cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt
     7861 cttgatgaaa cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga
     7921 atggcggaaa ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa
     7981 atggtggttc gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg
     8041 ggtttgcgcc cgatgacgcc agacggtacg ccggttgtcg ggcgtacacg ctttaaaaat
     8101 ctgtggctga ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag
     8161 ttgttaagcg atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta
     8221 gcgcgctaca gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc
     8281 taaggaaacg agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag
     8341 aatctgtcca ttgtccgcca ggccgcgccg cacgcgcgcg tctggtcggt ggtaaaagcg
     8401 aacgcttacg ggcatggtat tgagcgtatc tggagcgcgc tcggggccac cgatggcttt
     8461 gcattactta acctggaaga ggcaataacg ttacgtgagc gcggctggaa ggggccgatc
     8521 ctgatgctgg aaggattttt ccatgctcag gatctggaga tttatgacca gcaccgcctg
     8581 accacctgcg tacacagcaa ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca
     8641 ccgttggata tttatcttaa agtgaacagt gggatgaatc ggttgggctt ccagcccgat
     8701 cgcgtgctta ccgtctggca gcagttgcgg gcaatggcga atgttggcga gatgaccctg
     8761 atgtcgcatt ttgccgaagc ggaacatcct gatggaattt ccagcgcgat ggcgcgtatt
     8821 gagcaggcgg cggaagggct ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg
     8881 tggcacccgg aagcgcattt tgactgggtt cggcctggca ttattttgta tggcgcttcg
     8941 ccgtccggtc agtggcgtga tatcgccaat accggattac gtccggtgat gacgctaagc
     9001 agtgagatta ttggtgtcca gacgctaaaa gcgggtgagc gtgtgggcta cggcggtcgc
     9061 tatactgcgc gcgatgaaca gcgaatcggc attgtcgccg cagggtacgc cgacggttat
     9121 ccgcgccacg cgcctaccgg tacccctgtt ttagtggacg gcgtgcgcac catgacggtg
     9181 gggaccgtct cgatggatat gctagcggtc gatttaacgc cttgcccgca ggcggggatt
     9241 ggtacgccgg ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc
     9301 ggaacggtgg gctatgagtt gatgtgcgcg ctggcgttac gcgtcccggt tgtgacagtg
     9361 taacttgttg taagccggat cggagacagc gtcttctggt tgcgaaaaaa tcatccatcc
     9421 ggctggtcag caactgtagt tgttaatgtg acagagccat tgcccatgat agtgtccatt
     9481 aaaagaatgg acactatttc cccggaacct gaactcaccg cacaggcgtt ctacataaaa
     9541 cgcttacgct tcattgttga ctcgaactcg acttcagata aatcacgctt tacccttgat
     9601 ggagcctgta catagatttg tgtaattgcc tgattttgat atgttcaatt caacatcaaa
     9661 tgaaggttaa attatggacg acaaacaatt gcaggctcag gctgcgttca gcaaagcatc
     9721 gcaaccggcg atagatgctt cattaaattt aagattcagc ttcctcttca gccacccgta
     9781 cgccaatctt caacacttca ttatcttctt tctcggccac cgtccagatc atcccggcaa
     9841 actctacctg gtcaccgaca accggtgccg cgcctaacaa ctgctggaca atttcaccca
     9901 gcgtttgctg cttatcacga tattctcgcc cgtcttccag gccatatatc agcgcaacat
     9961 cagcatattt ggcgctggct tcgagaatga agtcaccaaa gaagcgttga tccagcgcga
    10021 ccggcggcga ctggctgaac agtttaccga gcgccggaag atcgcgttcc cgaccaatta
    10081 cacacaacac atcgccttcg cgcagtcggg tgctgccggt aggatgaagc aactggttat
    10141 cacgaaacag tgccgcaata cgcgtctctt ttggcatatg caaatcgcgc agtgccgcgc
    10201 ccacgcacca tttatcggca ctcaattgat aaacaaactg ctcccacgga ttttccggat
    10261 gaatatccag gccaacgcgt gacaccggac gtcccactgg cggaaccacc actttggctt
    10321 ttttagccgc ccacgagagt gatgttccct gcaagagcag tgaaaccaga accacaaaga
    10381 aggcgacatt aaagaacaga cgtgcattct ccagccccgc catcatcggg aacacggcca
    10441 ggatgattgg caccgcgccg cgtaatccta cccagctgat aaacacgcgc tcacgcagat
    10501 tgaaaccgcg gaaggggagc aatccagcaa ataccgaaag gggacgggcg aagaatatca
    10561 tccatgccga caaaatgagc gccggaatgg caataggcag cagatcgctt gggttaacca
    10621 atagccccag caccaggaac atggcgattt gcgccagcca ggcgaggccg tcgaaatttt
    10681 gcaggatgcc gtagcggttg cgaatcggcc gattacccag cagaaaaccg cacagataaa
    10741 cagccagaat accgctgcct tccagcgccg tggttaaagc aaaaattaaa ataccgccgc
    10801 ttaatgccag caatggatat aatccggcag gcagggcgat gcgattaatc atttgcagca
    10861 gtaaataacc accgccaagt ccgatgacaa tcccgaggcc aaattgctgc agaatatcga
    10921 ccacgaacat ccaactgaca ctgctttcat gttgctggat catcgcaatc agggtaatcg
    10981 tcagaaagac cgccattgga tcattactgc cggattcaat ttccagcgtc gagccaacac
    11041 gttcgttaag ccccttacca cccagcagag aaaagaccgc tgcagcatcg gtagagccga
    11101 cgatagcgcc gattaataag ccttcaatca aatcaagatt aaacagccac gccgccatca
    11161 tgccggttaa accagaggtg ataagcacgc ccagcgtcgc cagcgacagt gccggtccta
    11221 acgccacacg aaaggaactg gcctgagtgc gcatcccgcc gtcgagcaaa ataatcgcca
    11281 gtgccaggtt acttaccatg taggcgaagg ggtaattatc aaacgggata ccgccgacgc
    11341 catcgactcc tgccagcatg ccgatcgcca gaaaaataac cagaatagga atgccaagac
    11401 gggaagaaaa tgaactaagt aatatactgc tggtgacaag gatggatcct aagatgaaaa
    11461 ggctaattat tgttgtggca tccaacggtc ggttactcct gattacgctg tctcttatat
    11521 aaaccctacc atattagcgg cagagacagc gttttactta gccctgaagc gtgatttttt
    11581 tacattttaa gaacaggatg accgctaatc gtcaactgag tgccttcccg ggtgttattc
    11641 agaatggcat gtgcgcccaa cggcagcgta accgtgcgtt gttcatgacc aaaatcgagg
    11701 ccggtaatga gtggaatcga caggcgggaa cgcaaaaacg cgtacactga ctccaggttg
    11761 taacctgcgt cataatcatt gggcgtgctg ccgctaaagc taccgagaat aatcgccttc
    11821 tgacgtggca aaattcccgc atgatagagc tgcaacagca tacgttcgac gcggaaaggg
    11881 tgctcgttaa tatcttccag caccagaata ccgttctcaa tttttggcat ccacggtgta
    11941 ccaatcagtg aaatcagcat cgcaagattg cctccccaca acgtgccttc ggtctgacat
    12001 gtcgggcctt ccccttgcca ttcaatggtg aaggttttat tgcgtaacgc cagccagaaa
    12061 tggtgctcgg taaaggcgtt cagttcatcc gcaccaaagt ttgctaccag catggggccg
    12121 ctaaaggtga tgacattgcc ttgcgccaga agaccgcact gaatggcggt aaaatcgcta
    12181 tgtccgcaaa tgagtaacgg gtcatgttgt tggcgagcca ccagtgcctg ccagtcaata
    12241 tctgccagta aacgactggc accgtaaccg ccgcgtacag ccagcacgat ggtgttgggg
    12301 gtagtcagtc tggcaaggga attgagatct tccaggcgct ccgtttccgt acccgcaaaa
    12361 cgctcacaac gacgggcaat aacctcgacg ttattgacct gatgcccctc gtcggtaagg
    12421 cgatggatac cacgcagcgc ggcgtgctgt ttaatgcagt aacccgatgg ggcaattaag
    12481 tgaaacagag acatggcaat tccttgctga caacagaaac gaaatgtata tcatgccgct
    12541 taggtgtgcc gttgtcacct caacggcgat tccaggctat aaggatagaa gaagtgaaat
    12601 tgagatggtt tgcctttttg attgtgttat tagcgggttg ttcatcaaag catgactata
    12661 cgaacccgcc gtggaacgcg aaagttccgg tgcaacgtgc gatgcagtgg atgccaataa
    12721 gccagaaagc cggtgcagcc tggggcgtcg atccacaatt gatcacggcg attatcgcta
    12781 tcgaatcggg tggtaatccg aacgcggtga gtaaatcgaa tgccattggt ttgatgcagt
    12841 taaaagcttc aacctccgga cgtgatgttt atcgccgtat gggctggagt ggtgagccga
    12901 cgaccagcga gctgaaaaat ccagagcgta atatttcaat gggggcggct tacctgaata
    12961 ttctggaaac cggcccgctg gcaggcattg aagatccgaa ggtactgcaa tatgcgctgg
    13021 tggtgtcata cgctaacggg gcaggtgcgc tgctacggac tttctcgtca gatcggaaaa
    13081 aggcgatcag caaaatcaac gatttggatg ctgacgagtt cctcgatcac gtagcgcgaa
    13141 atcatcctgc gccgcaggct ccgcgctata tctacaaact tgagcaggca ctggacgcga
    13201 tgtaaatcag tcgcgcactt tgtccgcttt ttctcgggct tctcgctcga gagagaaaat
    13261 aatccgctgt aattgccgct ccaccgtcgg gctgacgtta agaaaacgga agctcagacg
    13321 gggagtggtg atggtttcat tcttgccatc aatcactttg cgctcgctga tggagattaa
    13381 ctgggcgtca aagtgaaaaa caccccattg ccccatgttg acttcaatct gagcgaagcg
    13441 catgccttct tgtaattcgg caggctttgc tgtttccagt aatgcgccca tgccgcctaa
    13501 cgacaaatca tacaggcgga aacgtaacgt actgttatcc gccagtttgg tctggcaaaa
    13561 ataaggcgga tggagtgggg cggagatgcg gaaatatcgg cgtcgttgta caaaccataa
    13621 ggtgggaggc ggtacggtga taaatgccgg aagctgcaag tattcactct gctgtagttg
    13681 ttcaacagta aactcgactt tcgcaccctg agtttcggcg gtaatggtaa tgtgctgtgc
    13741 ctttagcacg gcgatgttgt cttcggcttg actgccgaaa tccagcacca gtttatccgg
    13801 ggttattgcc agtattttgc tgatcagctg cccgccattc caactgagac gcaaaggaat
    13861 tgcggctttg tgcaaatcgc gtaacacgcc caggacggct aacggatttt gtttcaggaa
    13921 ctgctcatgg taatgactca cgcggaaaaa ctcctgactg acaaactgtc tttggtttat
    13981 cggtcacagt taacaaaact taatacaaat gtgtgaatat tttttacatg ctgttcttaa
    14041 atcagccgac atacgcccag catcgatccc gcccttgcct atacttagag cgttgatgta
    14101 agcatttctt gcgtccgatt catcgaaacg agggcctgaa catgggaatt attgcctgga
    14161 ttatttttgg cctgatagcc ggcattatcg ccaagctaat catgccgggg cgtgatggtg
    14221 gtggattttt cctgacctgt attctcggga tagtcggtgc ggtggtcggc ggctggctgg
    14281 cgaccatgtt tggcattggc ggctccatca gtggttttaa tctgcacagc ttcctggtgg
    14341 cggtggtggg agctattctc gttctgggca tattccgcct cctgcgaaga gaataagatt
    14401 ttcataaggc ggatagcgat acagatgccg ctatccgctt tcacatcaga acgtatactc
    14461 gacacctgct ttacgggtgg aacaaatcaa tatagcactt agatgcccga taaagtcggt
    14521 tcccggtaac aacaacagaa tcctttttta attattgttt cgttgttttt ggtgtgattc
    14581 agaaaataaa tattttagac attagccagt caggcgttaa aaatggggcg gatagtgagg
    14641 cggttttggc tgcgcttatt gaacatttag aattgatcaa tccttctggc agattaagcc
    14701 aaaatacccg ttcggcaatg cttcagttac gcgaagagga atggtctgaa ttcttttttt
    14761 ggttgctaaa ttctctggag tgtctggatt atgtgattat taatttaact ccagaaagca
    14821 aaaaaacgtt gatgagtgaa caccgtaaca atattcaggt agcaatcgat gctttatata
    14881 ggcaacgtcg gtgtaaatca ccgggtgatg aaagtgaaac acttacccgt agaaatgacg
    14941 ctatcttcgg caaccacgta tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa
    15001 aaccgtctgt gtagaaaaca ccatagctat ggtgttatct gccgacaacg ccatccggag
    15061 aagccgaatg acgttgtcgg agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt
    15121 gagggttgcg tcgttgctga cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt
    15181 tgctctttcg ggcagatcaa atccagcatt ttcagcgtca cgccattggt ccagccaaag
    15241 ccatcctgta atggatattc gccaccgccg ccccccgttc cggtggtgct gacatcatat
    15301 ttttccacca gctttttctc ccggtcatag gtgtgctgaa cattggtcag gaagtgccag
    15361 ctaatgtcca tcgccacctc tttttgcccg tagttttgta atccttctgt cgcgacccac
    15421 tgtaacggtg cccagccatt tggcgcatcc cattgttgcc cacttttcac cgacgtggtg
    15481 ttcaggccgc cgggttgcag cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga
    15541 tctttcgctg ccgcattgac gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc
    15601 actttataac ttttcaggtc gtaatcggca taccagcctt gttgatcgtt ccacatgtat
    15661 ttttcgatcc ctttttgacg ggcatttgcc agcgtttcgt actggtttgc catcgcgtta
    15721 tctccggcag ctttgctggc gcgggcgagg attttttcca ttttaaacat caggctgttc
    15781 agatcgaccg gtacgatgct ggtggtgcgt aaggtattta gttgatgcgg gttgtccatc
    15841 cagcgcgagc tgaaatccca gccagacgca gcggcagagc gcaggtcgcg gtaaatttca
    15901 gtggcaggtc gattcggatt gcttttggcg gtggcaatat cttccaccca tgactctggt
    15961 cgtggcgtat cgcgatcgtc ccagtagcgg ttgagaaggg taccatcctg aagttttaca
    16021 acgcgttttt cctgttgtcc ggcttgcagg ttttcaacac cgtccatcca gtaagcatat
    16081 tctttttgca tttgcggcag gtattgcttc aacgcggcat cgccttcatg ctgcgccagt
    16141 aactctacca tcagggcaaa gaagggcggt tgcgagcggc ttaaatagta actgcggttg
    16201 ccgttgggaa tatgaccgta agtgtctatt tcatgagcaa aattggccac catatccgcg
    16261 actttatccc agtgaccgct ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa
    16321 tatacctcgc gaaaacgtcc gcccggcacg acataaggtt ccggcagcgg taacagagaa
    16381 tcccattttt cggtgttttc ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt
    16441 tcgcgcagtg actgcccctc tggcggaaca tatttctcgc cttctttcgg cagggtgaaa
    16501 ttgacgttaa cgaaatggcg cagatcaaat ccgctctggt tttgctgcat ccgataatca
    16561 gcaaggatca tcagcggatc gctgttcggc acggcatcgg caaaggtttt ttggtccgga
    16621 aaaagtttgg cgttttgcac atcattaaac agcggcccta ataaaatatc aggcggctgt
    16681 ggtgttaccg gtgtttcttc tgcctgcacc gatagcgcag cgaaacacaa aaagatacag
    16741 gctggaatta acgccatttt ttgcgggcga gaaggtgcgg gggatttcat caatcattct
    16801 ccttaggcga aaccgaataa aacggtattc aagtatcaga aaaccttagt tcaggatcgc
    16861 actagctgca tgatcgaaat ctcattctgc gaactgtcag gcattttaga aaagggaatt
    16921 aaagggataa ataacggcga aatagagtga tcaaataacg tctgtttatt agccagccag
    16981 cgacatttgg ttcgcgatta gcgaagaaaa atcatcataa attggtgaaa aatataacag
    17041 gtagttaaaa aacattagtg ctgagtaaat tgccggatga catcagaacg atgccatccg
    17101 aacggtggct taaccctgac ggttgaaacg ttgcgtttta acgtccagcg ttagcttttc
    17161 ttctggttgt atcgcataca gtttctcacc ctgctggcaa atccagccaa tctccagttc
    17221 acgggcgatt agggcgctgt gggataccgg acttccggcg ctaaggcaga taccttttac
    17281 aaccgccgga tccagttgca gtactgtgga aggataaatg ttctccgcca gtagaatagt
    17341 tggcgagtta aactgcggga gttcttcttt cgtttgggtc aggtggacca gggtgcgatg
    17401 cagaagatcg tccacatcaa tatagcgagc ttgtagatat tcatcatcca gttgctggta
    17461 ttgctggcta agttctttaa gaacttgctg ccaggcatat tctgccgtgc aatgttcgtg
    17521 ctgaaggagt tcgcttgccg ccgccagcag ttccggatca tctaacagtg tatggtgacc
    17581 agaaaagatt gcggcaatat cgtcaagccc gctggcttct gctttcgctg ttaacgtcat
    17641 cagatctaat aacgtgaagt caatggcctg gcgtaatcga tcttgttcct cttccacggt
    17701 cagggttgat ttggcctgta ccgtacataa aaccggttga taataaaatg ctttacccga
    17761 aacaggcgga acgggacgca gagtaggtgg agcaacttcc tccgtttcac caaagttatc
    17821 ttcagccagc tgacggaaag cgatcagtgc ctcttcagct tctggccctt tcgcaatcag
    17881 gcgcagcgta tcgttatagc gaacttgtag taacgcaatc tggttaatac tctccggtgt
    17941 gacgcatttg ccgtttttct ccagcaacat atcggcatta aatgtcgata aggtataaac
    18001 cagccgggag gctggacgta catgcaggcc gttacggttc tttatgacca ccgccagaga
    18061 acgggcttct tcatcgtacg caggacatgt gtcagagatt tcagtatcgg aggacagtaa
    18121 acccagttgt tcacgtttgg cttccagcgc atgcatggcg tcaaagataa ctttgtcgat
    18181 atccgccccc gaggccgcgc tgaccgttgc tgccagtgta ccttcgacca acggcgcagc
    18241 acacaaacgt acttttgcgg cgatctcggg agccagcaat tccagcgcag tttcagcact
    18301 caataatgcg ctacccatat ccatcatgac cagcacatgg tcggcatcgg caacagattc
    18361 gatggcctcc atcactttga cggcatcggt accaatggga ttttgtggat cgtcaattcc
    18421 cgcggcaatg gcgattttac aactatcact cattaacatc tgacgggcta attcaccgac
    18481 accttctccc agtcggctgc tatgtgaaac tatgaccagg tttaccatca ccaattcctt
    18541 actcttttgc ggctaacgcc aacatttgca tcataaacat caccgaggtt gcgccgggat
    18601 cctggtgacc aatactgcgt tcaccgagat aactggcgcg gcctttgcgg gcttgcattg
    18661 taatcgtact ttgtgcagcg gattcggcga tgctactggc tgcttcgagc gccgccggaa
    18721 cagagagatt ttgctcgctg gactgacgta acgattccac caccggcacc cacacatcac
    18781 acatggtttt atcgccaggt tcggctttcc cgcgactgat tacgccgtcc gcgccatcgc
    18841 ggaacatctg ataaagctct tccagcgtca ggctttgccg tgcctgggtc gcctgtgctg
    18901 cgcgtataaa gaaagtaccg aacagcggac cactggcacc accgacgctg gaaagcagcg
    18961 tcataccggt attcttgaga atgaaaccga tatctttatc tgcgatagca gggagttttt
    19021 ccaccacttt gctaaagcct cggttcatat ttagcccgtg gtcagcgtcg ccaatttcgc
    19081 gatccagtcc ggtaagatac ccgctctcgg tgttgaaaat atcgccacaa cgagtgagcc
    19141 agttaacaat ttgagttctg ctcagtgaca ttgctttctc cttatttacc ccagttaagg
    19201 gccggggtgt ggaccggggc gtcccagaga gccagcgttt cgtcatcaac tttcagtaag
    19261 gtgattgaga aaccggtcat atccagtgag gtgcaatacg cgccaattaa attacgctcg
    19321 atagtcaatc ccgcttgctg gcaacgtgtg gtcaggcggt tatagacgcc gtacagctca
    19381 gaaagcggag ttgcgccaag attgttaacc agcgcaatca cccgatcgcc agactggagc
    19441 tgttgtttgg tttgtggttc ttcctgccaa ctgccttgtt gataatccca gaaacgcaaa
    19501 gtgcgatggt atgagccatt ttccagcagg gtgtcgaaca tttcatcgac ggtttgatca
    19561 agggaagaga aggggcggcg gtcaataccc ggctcaccat gaatgccgac gccaaactcc
    19621 atctcattgt ccgccagggt aaaagaaggt ttgcccgcgg caggaacggt acaggcaccg
    19681 agagcgatac ctattgagtg gccttgatta ttcaacttac gccccagttc cgcacaggcg
    19741 tccagtgagt cgccacgctc cgccgctgcg cctacgagtt tttcaattaa tacggtgttg
    19801 gcaacgccgc gtcgcccggc ggtataaaga ctgtctttta ctgcaacgtc gtcatcaatg
    19861 accacagtgg tcacttttac gccgctatcg tgcagtaact cggttgctgt ttcgaagtta
    19921 agaatatcgc cggtgtaatt tttgataatc aacagtacac cttcgccgcc atcaacttgc
    19981 atggcgcatt caaagatttt atcgggcgtc ggtgaggtga aaatttcgcc cggacaggcc
    20041 cccgaaagca tcccctgacc gatataacca cagtgcatcg gctcgtgtcc gctgccgcca
    20101 cccgacagca gggcgacttt tcctgcaacg ggggcatcag ctcgggtgac atacaccggt
    20161 tcctgatgca gtgtcagcga tggatgcgct ttcgccagtc ctgccagttg ttcgtccagt
    20221 acgtcttgca catcattgat caattttttc attattttgc tccagcaatt acggtagggc
    20281 atggatgatg ttcaacgaca cggcgaccac tgactgccga tgaatccatt gtgcatcagg
    20341 caagggaaaa gaaaattagc ccaaattatg tttcatagtg aaacatatgc tttaacgaat
    20401 gttccatatt gaaactttta cgtgtattaa tacttaaaat tgcgagccgg aacacctttt
    20461 gtcataaggg atgcgggata tgagtggcgc ttttaacaac gatggtcggg gcatatctcc
    20521 cttaattgca acctcctggg agcgatgcaa taagctgatg aaacgggaga catggaacgt
    20581 accacatcag gcccagggcg tgacatttgc ttctatttat cggcgtaaga aagcgatgct
    20641 gacgctcggg caggctgcgc tggaagatgc ctgggaatat atggcaccgc gagagtgtgc
    20701 gctgtttatc ctcgatgaaa ccgcctgcat tctcagccgt aatggcgatc cgcaaacctt
    20761 gcagcagcta agtgcactgg gattcaatga cggcacgtat tgcgccgagg gaattattgg
    20821 tacttgtgcg ctatcgttag cggctatctc tggtcaggcc gtgaaaacga tggccgatca
    20881 acatttcaaa caggcactct ggaactgggc cttttgtgca acgccgttgt ttgacagcaa
    20941 gggccgattg acgggaacaa tagcgctggc gtgtccggtt gagcaaacta ccgcagctga
    21001 tttgccgttg acgttggcaa tcgcccgcga ggtcggtaat ttactgctga cggacagttt
    21061 gctcgctgaa actaaccgtc atttaaatca acttaatgcc ctgttagaaa gtatggatga
    21121 tggcgtgatt agctgggacg agcagggtaa tttgcaattt attaatgccc aggcggcgcg
    21181 ggtcttgcgc cttgacgcga cggcaagtca gggacgggca atcactgaac tcttaacgtt
    21241 acccgccgta ttgcaacaag caataaaaca ggcacatccg ctcaaacacg tagaagcaac
    21301 ctttgaaagt cagcaccagt ttattgatgc ggtgataacc cttaaaccga taatagaaac
    21361 gcagggaacc agttttattt tgttgctcca tcctgtggaa cagatgcggc agttgatgac
    21421 cagtcaatta ggaaaagtca gccatacctt cgctcatatg ccacaggacg atccgcaaac
    21481 ccgccgcttg attcattttg gtcgccaggc ggcgcgcagt agctttcctg tcctgctttg
    21541 tggagaagag ggcgtgggca aggcactgct aagtcaggca attcataatg aaagtgagcg
    21601 tgctgcaggt ccttatatcg ccgtcaattg tgagttatat ggtgatgcgg cactggcgga
    21661 agaatttatt ggtggcgatc gcacggacaa tgaaaatggc cgtctgagtc ggctggaact
    21721 ggcgcacggc ggcacgctgt ttcttgaaaa gattgaatat ctggcggtgg agttacagtc
    21781 tgctttgctt caggttatca agcagggtgt tatcacgcga ctggatgcgc ggcgtttaat
    21841 accaattgat gtcaaagtga ttgccacaac gaccgcggac ctcgcaatgc tggtggaaca
    21901 aaatcgtttt agtcgccagc tgtattacgc gctgcatgca tttgaaatta ccatcccgcc
    21961 tctgcgtatg cggcgtggca gcattccggc tctggtgaat aacaaattac gcagtcttga
    22021 aaaacgcttc tctacgcggc tgaaaattga tgacgatgcc ctcgctcgcc tggtttcctg
    22081 tgcatggcct ggcaacgatt ttgaacttta cagcgtcatc gagaatcttg ctctgagtag
    22141 tgataatggg cgtattcgcg tcagtgattt gccggaacat ctgtttaccg agcaggcaac
    22201 agatgatgtc agcgccacac gcctttccac cagtctgtca tttgcggaag ttgaaaaaga
    22261 ggcaattatt aacgcagccc aggtcacagg cggtcgcatt caggaaatgt cggctttact
    22321 tggaatcggc cgcactacgc tgtggcggaa aatgaagcaa catggcattg atgcagggca
    22381 gtttaagcgc cgggtatgaa agacagagac gatttctgat acatcagagt gatctatatt
    22441 tcattccggc gcacgctaac atttttcagc atcgtttaag ggcttgtcta ccccgtactt
    22501 aaaagctgaa gtgatatcct ccgttgactt gcttctgatc aaagaggtta ccctgcgtgt
    22561 aatccgcttc gaggtagaat gtgtgctgtt tgttatactg tgcactgact cccacgccgt
    22621 tattccagcc attacctttg aaactgtact tctcccggga gttgttcaac agatattctg
    22681 tatcccctga aaactcgcgg atagcgccag tcttcacata catattcagc tggctgttgc
    22741 ctgcggtgat gtcatatccc agtatcatgc tggcacgccc cagcagcgat tcgtagtgat
    22801 tcagatgtat attgagacca ttactcgcct tcatagccat ctcattctgg tggctgtatg
    22861 tgagctgggt ttgcggctct atatagaacc cataaccagt aggggtcagg ttgaacctct
    22921 gcccggcttc cagggagatg ctcaatccat tcgcagtgcc gttggcgtta acgccgttgt
    22981 tctgactgtc ccgtacgtgg aaactatttt tctggcgcga tgcttttaca acgagatcgc
    23041 tgtaaaaacc gttgtgtgcc atgtaactgg cgtacattcc catgtagtct gaacgtgcgg
    23101 taccgtcgcc accgctatag tccggcgatg catgtgttga gccaatatac agaccgacat
    23161 acaacggcat tacatcagag agacgtttat ccccaccaaa ctggataccg ctgtaaccca
    23221 tgtcaaagcc gctcagtttg ccactggcga aggagtccag gcttccccca taactgcgca
    23281 gccagatatt accgtcttta ctctgatttc gcaggtcacc cattcgttgc atcagcgtac
    23341 ggttttcaac atagtttaat aagtaaccga cattgagata attgccgcca gcatctgcgg
    23401 tcgtggtggg tttaggtgtg ggagtggggg taggcgtagg atcgggttca ggcgtaggat
    23461 cggggttgac tatgggaggc tgagcgggag ccggtgttgg ttcaggatta ggagtcggtt
    23521 ccggaactgt cccggaagcg taaagctccc agttagtgcc atttttacgc acatcataca
    23581 gatatccccc caactcaacc tgagaagacg cgctgaacga ggccgcgccg tcagtggttt
    23641 ttaccactgt cagaacttca tttcccgttg tggcttcgct gccctggtta cggatagcca
    23701 atacatgatt accagcacta ctcccgctga tattcaataa atccccttta ttattaacgc
    23761 cattaccctc accaacaaca tcagcacgca taataaaggt actgttaccg ctcaggttct
    23821 ctacgtttaa tgtggtaaat gtgccggcag ttgatgcgtg gctggcaaaa tcgacagttg
    23881 aatggctcag cgccagcgtg tcgagattag aattatttgt tacgttccag acgctgttat
    23941 tcattgcaac gtccagtttc ccgccattga cattatcgct gagggaggaa cctgtccaaa
    24001 ccgacccaga gtgcatatcc agattgatta gcccaccttt ggaaagaacg ctaccgttga
    24061 taagcatacg acccgaggcg ttgatgcggc tggcggcata accatcgtca tgttgcgttg
    24121 cgatggccat ctggtcgggt gtactcatat caatgaccag atcgctcgtg aggtcgatct
    24181 ggctgttggt catggcataa atccccctgg ctcctgccgc gccggtgata gccaaactgt
    24241 ctccggttat tctaccgccg ctgagcgccc acaaacccag cgccagactg ccattacgat
    24301 caaccgtaat atcggtattt tgcatgttga taacagccgt tgccgtctgg gccgaggcac
    24361 cataagaacc gccggaaaag atgctgtttc gttgtgctgc cgtgccagaa aaattgattg
    24421 tcgcgtctga accactggct acgaggccac cgccctgcgc ggaagaaata tggctatctg
    24481 caccgattgt ggttgtacca ccacgaactt cgacgccatt ggccgcggct ccagttacat
    24541 caacagtgag tgcattcgcg ctaacctggc caaagctcca gaggccgtgt gcattatcgc
    24601 cactggtttt aatggaactg tttgttccga gatcgacaac agagtttttc tgtacgttta
    24661 tccccatggc gctgtagccc tgaacatcga ttgtcaggtc tgtcgccgta aaacgcgcag
    24721 caccattggc gttattgccg ttgagaccac cgatataaac acctgtactt ccatcggtct
    24781 tgattttact tccgcttcca agatcgacac tggtgccata gtcattgatg gttaggccta
    24841 taccgttcga gttttcaatg gtgaattgag tggctgttag tgttgagcta tgcccaataa
    24901 tgatgccgtc atcgttactc ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt
    24961 caccaattaa attaatgccg atggcagagg tttgaccaac aacatctact gtgagtcggt
    25021 tagctgtcag ggagctgttt ttgccctgaa taatgattcc ttttgcatat ccagaggcgt
    25081 cgttgacatt gacggtgaca tcattaccca ggttgatatt gccaggcgtg tttcctgctg
    25141 gggtcattac accatagagg tttcctgatg gatcggcggt atctcctgta attctgtcac
    25201 catcattaat agtaatattg gtgccatatt gacttcctgt atcagcagct atcagcaggg
    25261 gggaaaagat aattgcattc aaaccaatag cgccaaattt tatcagttga attgagggcg
    25321 aacggatttt taattcagcg agacgatcgg ctttggtatt cccattgtgt tgtttgatgc
    25381 ccataccact cctatatagt acccaggcta tgggtctatg gattttgtca ttttgtttaa
    25441 gtcaatgaat aatatcctc
//
LOCUS       DAPHJE010000063        25390 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPHJE010000063 DAPHJE010000000
VERSION     DAPHJE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.063"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(101..2062)
                     /gene="topB"
                     /locus_tag="RH222_002624"
     CDS             complement(101..2062)
                     /gene="topB"
                     /locus_tag="RH222_002624"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV3797626.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNVISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2067..3110)
                     /gene="selD"
                     /locus_tag="RH222_002625"
     CDS             complement(2067..3110)
                     /gene="selD"
                     /locus_tag="RH222_002625"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV3797627.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3227..3778)
                     /locus_tag="RH222_002626"
     CDS             complement(3227..3778)
                     /locus_tag="RH222_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV3797628.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            3939..5795
                     /gene="sppA"
                     /locus_tag="RH222_002627"
     CDS             3939..5795
                     /gene="sppA"
                     /locus_tag="RH222_002627"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV3797629.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSSSDTKETAGRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKDFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDTFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5962..6978
                     /gene="ansA"
                     /locus_tag="RH222_002628"
     CDS             5962..6978
                     /gene="ansA"
                     /locus_tag="RH222_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV3797630.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDEYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            6989..7630
                     /gene="pncA"
                     /locus_tag="RH222_002629"
     CDS             6989..7630
                     /gene="pncA"
                     /locus_tag="RH222_002629"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV3797631.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7723..9081)
                     /locus_tag="RH222_002630"
     CDS             complement(7723..9081)
                     /locus_tag="RH222_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3797632.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9198..9956)
                     /locus_tag="RH222_002631"
     CDS             complement(9198..9956)
                     /locus_tag="RH222_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3797633.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10093..11073)
                     /gene="ydjG"
                     /locus_tag="RH222_002632"
     CDS             complement(10093..11073)
                     /gene="ydjG"
                     /locus_tag="RH222_002632"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDV3797634.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGNDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            complement(11083..12030)
                     /locus_tag="RH222_002633"
     CDS             complement(11083..12030)
                     /locus_tag="RH222_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV3797635.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFSRFSQAKLLSLPSIFNSPLLDGKALTEIFTQA
                     KTRQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12035..12871)
                     /locus_tag="RH222_002634"
     CDS             complement(12035..12871)
                     /locus_tag="RH222_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDV3797636.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(12892..13935)
                     /locus_tag="RH222_002635"
     CDS             complement(12892..13935)
                     /locus_tag="RH222_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDV3797637.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTIKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(13952..15331)
                     /locus_tag="RH222_002636"
     CDS             complement(13952..15331)
                     /locus_tag="RH222_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3797638.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(15358..16434)
                     /locus_tag="RH222_002637"
     CDS             complement(15358..16434)
                     /locus_tag="RH222_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV3797639.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(16804..17076)
                     /locus_tag="RH222_002638"
     CDS             complement(16804..17076)
                     /locus_tag="RH222_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV3797640.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(17118..17531)
                     /gene="msrB"
                     /locus_tag="RH222_002639"
     CDS             complement(17118..17531)
                     /gene="msrB"
                     /locus_tag="RH222_002639"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV3797641.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            17873..18868
                     /gene="gapA"
                     /locus_tag="RH222_002640"
     CDS             17873..18868
                     /gene="gapA"
                     /locus_tag="RH222_002640"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV3797642.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            18952..19836
                     /locus_tag="RH222_002641"
     CDS             18952..19836
                     /locus_tag="RH222_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV3797643.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(19887..20741)
                     /gene="yeaE"
                     /locus_tag="RH222_002642"
     CDS             complement(19887..20741)
                     /gene="yeaE"
                     /locus_tag="RH222_002642"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDV3797644.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYIGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(20831..21577)
                     /gene="mipA"
                     /locus_tag="RH222_002643"
     CDS             complement(20831..21577)
                     /gene="mipA"
                     /locus_tag="RH222_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV3797645.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22013..23947
                     /gene="yeaG"
                     /locus_tag="RH222_002644"
     CDS             22013..23947
                     /gene="yeaG"
                     /locus_tag="RH222_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV3797646.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24060..25343
                     /locus_tag="RH222_002645"
     CDS             24060..25343
                     /locus_tag="RH222_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV3797647.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNSTAKD"
BASE COUNT         6526 a         6553 c         5939 g         6372 t
ORIGIN      
        1 aggcgataat tttatccatg caaaaaaaat atccaacgaa ttttcttgat ccgctggata
       61 tttcagaata tgactcgaat agcacgaaag attcgctcgc ttacgctatc gccccgcttc
      121 cggcttcatc tgctggcgga ctttttttcg cactacgttt acgcggtgca gcctttttct
      181 tatcagcact gccaccactg cccggagcca caatgccgcg aaactgccgc accggagtac
      241 gtttggcttg atcaataagc tgatatagcg tccccaccag cggctgcata aagtcctgat
      301 agcgacactg cttttcgctg atttgcgtca gcaccgattc ccagtgcgcg gtcatgtccg
      361 gtcgcgtagc catttctggc agcgaatgga ataacgcttt tccggcgtcg gtggagtgga
      421 tatagcgccc ttttttggtg aggaaaccac gcttgaacaa cagttcaata atcccggcac
      481 gcgttgcttc tgtccccaga ccatcggtcg cacgaaggat ctttttcaga tctttgtcct
      541 gcacaaagcg cgcaatcccg gtcatcgccg aaagcagtgt tgcatcggta aaatggcgcg
      601 gcggctgggt ttgccgctct accacttcac ctttttcaca cagtaactca tcgcctttcg
      661 ccaccacagg caatggcgtg ccgtcgtttt cttcatcgcg ctctttgctg cctaacagcg
      721 cgcgccaacc tgcttcagca agaaaacgcg ctttagcgac aaatttgcct ttggcaatgt
      781 ccagttcgat aacacacttg cggaacaccg catccgggca gaactgcatc agatactgac
      841 gggcaatcag gttatagacc ttcgcttcgt tctccgtcag gttgatcgca gaactccgtg
      901 cggtcggaat gatggcgtgg tgcgcatcga cctttttgtc atcccaacag cggttgcgta
      961 tatctggatc taccactggc tgcggcaaca gatctggtgc atgaacgctg atgacattca
     1021 tcaccgcgtg gcgtccggca aaatgttctt ctggcaaata gcgacaatcg gaacgcggat
     1081 aagtgattaa cttgtgtgtt tcgtacagct tctggcagat atcaagcacg ttctgcgcgc
     1141 tcagaccaaa gcgttttgcc gcttcaatct gcaacgccga aagcgaaaac ggcagcggcg
     1201 cggattctga ttcccgttta tcgttatagc tggtgacaat agccggttga ccactaatgc
     1261 ggttaaccac atgctccgcc agtggacgat gtaacaagcg cccttcttca tcctggtacg
     1321 gttcacacgc ttcgctcggt tgccagatag cggtaaaccg ctcatcggca ggtgtcacga
     1381 tatgcgcttt gacttcaaag aagtctttcg ccacgaagtt ttcaatctct tcatcgcggc
     1441 gcaccaccag cccgagcacg ggtgtctgca cgcgtcccac ggaaagtacg ccctgataac
     1501 cggcattgcg accgagaatg gtatacgcac gggtcatatt gatgccgtac agccagtcgg
     1561 cacgcgctcg cgccagcgca gaaacgcaca atggcacaaa ctcactgttg gaacgaagac
     1621 ggtcgatcgc ccgctcaacc gcctgcgggt tcaggtcgtt tatcaagcaa cgctgcacct
     1681 gctggcgctt ttccggtgcc agttgcagat agtccagcac ttcatccacc agcaattgcc
     1741 cttcacgatc cgggtccccg gcgtgaacga tttcgctggc ttcatgcagg aagcgtttga
     1801 tgacgttaag ttgtttggtc acggagggtc ggggctgtaa ttgccacttt tccgggacaa
     1861 tcggcaaatc cgcaagattc cagcgcgcat agcggctgtc gtaggcgtct ggctgcgcct
     1921 gctcaagcag gtgaccgata caccaggtca ccacctgacc attaccgcac tcgataaagc
     1981 catcgccttt acggtgcggt ttgggcagga catcagcaat ggcgcgcgcc agactcggtt
     2041 tttcggcaat aaacaaccgc attgaattaa cggatctcaa ccatggcacg accgccgcgc
     2101 gctggcacca gttcgccaat cgccgtcagt tcaatgccaa actcggcggc tgtagtttta
     2161 acctcatttt ctgcttccgg catgaccgcc agcagcaaac cgccagaagt ttgcggatcg
     2221 cacagcagat cgcgcacttc acgcggcatt tcacccatca gatgaccgta gctggcaaag
     2281 ttacgttcgg tgccgccagg cactgcgccc aacttaatgt actcttcaac ccccggcaat
     2341 tttgggatcg cgtcatagtc aacgcgcgcc tgcacacctg cgccctgaca catttcgctc
     2401 aaatggccca gcagaccaaa accagtaacg tcggtcatcg cttttacacc ttcgatgttg
     2461 gcaaaggacg cacctgcgat gttcatccgg cacatcactt ccgtcgccag tccctgatgt
     2521 tctggtttca acagtgattt tttctcagcc gtggtaagaa cgccgatccc cagcggtttc
     2581 gtcaggaaca gtttgcatcc ggcttgtgcg gtactgtttt tcttcacccg ctcggtcggt
     2641 acgatccccg ttaccgccag accaaaaatc ggctccggcg catcgatgga gtgaccgcca
     2701 gccagcgcaa tacccgcctg acggcatgca tagcgtccac cttcggttac ttcgcgggca
     2761 atttctgggg aaagtttgtt aatcggccag ccgaggatcg caatcgccat aatcggtttg
     2821 ccccccatcg cgaagatatc gctgatggcg ttagtcgccg caatgcggcc aaaatcgaaa
     2881 ggattatcaa cgatcggcat aaagaagtcg gtggtactga taacgctggt gccattgccc
     2941 agatcgtaca ccgccgcatc gtcgcgggtc tcattaccca caagcaaatt cggatcaaca
     3001 aacttcgcct gctcactgtg caggatggtt tccaacactt ttggggaaat tttacagccg
     3061 caaccagctc cgtggctgta ttgggtcaaa cgaatcgagt tctcgctcat ggacatctcc
     3121 tgtcaatgca atccgggtat ggtaaccctc attccgtgaa gtgataagtg agagtgtctg
     3181 aattcctgcg cctttgctca caatccagac agtttcgcga caattatcag aaataagtta
     3241 caaacggcgt cgggtccggg acgttaatcg acgtagatgc tttcaactgc ggcgtgccga
     3301 ggtagagaaa accgacaatt ttatcctgct cacggcaacc gaatgcttca cgcactaccg
     3361 gactttcagt taatgcgcca ctgcgccaga tgccgccaaa tccttgggca acggctgcca
     3421 tttgcatcgc catgaccgcg catccggcag acatttcctg ttcccatcgc gggactttat
     3481 gattttcttc gcatttcgcc accaccgtga tgatgagcgg tgcgcggaac ggcgcattac
     3541 gggctttgtc gatagctttg tcatcactac cggcagcaat cgccccctgt tccagtacgg
     3601 cgctgaaacg ctcgcgccct tccccttcaa tcacaaaaaa atgccacggt tgcatggact
     3661 tatggtccgg cgcacgcata cccgcacgca ggatgttttg cagttgttca cccgttggcg
     3721 cgggttcagc caggcgggag gcgctacggc gattgatcaa tagttcgagt gcatccattt
     3781 gattaactcc tgtcgtgata tttattcaca aaattaacac gagagtggat tttgttacag
     3841 cacagtccgc aattcctgct gacaagtgcc ggttgggtca ttacgataac cacatctatt
     3901 gcgcctgtga caggtgtgac cttaagttgg gagaatacat gcgaaccctt tggcgattta
     3961 ttgccggatt ttttaaatgg acgtggcgtc tgctgaattt cgtccgtgaa atggtactta
     4021 acctgttctt tatttttctc gtactggttg gtgtggggat ctggatgcag gtcagtagta
     4081 gcgataccaa agaaaccgcc ggtcgtggcg cactactgct cgatatttcc ggcgtgatcg
     4141 tcgataagcc cgacagttcg cagcggttta gtaagttaag ccgccaactg cttggcgcca
     4201 gttccgatcg tctgcaggaa aactcgctgt ttgatatcgt gaacacgatt cgtcaggcta
     4261 aagatgaccg gaacatcacc ggtatcgtga tggatttgaa agactttgcg ggaggcgacc
     4321 aaccgtctat gcagtacatc ggcaaagccc tgaaagagtt tcgtgacagc gggaaaccgg
     4381 tttatgccgt cggcgagaac tacagccagg gtcaatatta tctcgccagt ttcgccaata
     4441 aaatttggct gtcaccgcaa ggcgtggtgg atttgcacgg ttttgctacc aacggtctgt
     4501 actacaaatc gttgctggat aagctgaaag tttccaccca tgtgttccgc gtgggtacgt
     4561 ataaatcagc cgttgaaccg tttattcgtg atgatatgtc accggcagcc cgcgaagctg
     4621 acagccgctg gattggtgag ctgtggcaaa actatctgaa tactgttgcc gctaaccggc
     4681 agatccctgc tcagcaggta ttccctggcg cgcaagggtt gcttgagggt ttaaccaaaa
     4741 ccggtggcga taccgcgaaa tatgcgctgg aaaacaagct ggtcgatgca ctggcatcga
     4801 gtgccgaaat cgaaaaagct ctgaccaagg agttcggctg gagtaagact gataaaaatt
     4861 atcgcgccat cagttattac gattacgcat tgaaaacgcc ggcagatacc ggtgacagca
     4921 tcggtgtcgt cttcgctaat ggcgcaatta tggatggcga ggaaactcag gggaatgttg
     4981 gcggtgatac cactgcggca caaatccgcg acgctcgcct tgacccgaaa gtgaaagcga
     5041 ttgtcctgcg tgttaatagc ccaggcggca gcgttaccgc gtctgaagtg attcgcgctg
     5101 aactggcagc agcccgggca gcgggtaagc ctgtggttgt atcgatgggc ggcatggcgg
     5161 catctggtgg ttactggatt tccacaccag ctaattacat tgtggctaac cccagcaccc
     5221 tgaccggttc tatcggtatc ttcggcgtga tcaccaccgt agaaaatagt ctggattcga
     5281 ttggtgttca tactgatggt gtctcaactt caccgctggc ggatgtttct atcacaaagg
     5341 cactgccgcc ggaagcgcag cagatgatgc agttaagcat tgagaatggc tataaacgct
     5401 ttatcacgct ggttgctgat gcgcgtcatt cgacgccgga gcagattgat aaaatcgccc
     5461 agggccacgt ctggaccggt caggatgcaa aagctaacgg gctggtcgat agtctcgggg
     5521 atttcgatga tgcggtcgcc aaagcagcag agctggcaaa agtgaaacag tggcatctgg
     5581 aatactacgt tgatgaaccg accttcttcg acaaagtgat ggacaatatg tcaggttctg
     5641 tccgggcaat gttgccagat acgttccagg ccatgttacc tgcaccgctg gcctcggtag
     5701 cctctaccgt taaaagtgaa agtgacaagc tggccgcgtt taacgaccca caaaaccgtt
     5761 atgcgttttg cctgacctgc gccaacgtgc gttaagtctt gtactgagtg gccgacagat
     5821 cgtcggccac attatttttt acgtcgacga atcctcttcc cgctgtctcg ccccatattt
     5881 ccttatccgc atagtatcag gtgcgctccc cctgcctcac gtatatactt ttgctctttc
     5941 gatatcattc atatcaatat catgcaaaag aaatcaattt acgttgccta cacgggcggg
     6001 accatcggga tgcagcgttc cgagcagggt tatataccgg tgtcaggtca tctacaacgc
     6061 caactggcgc tgatgccgga attccatcgc ccggagatgc cggatttcac cattcatgaa
     6121 tatacgccgc tgatggattc ttctgatatg acgccggaag actggcagca tattgctgaa
     6181 gatattaaag cgcactatga cgagtacgat ggttttgtca ttctgcacgg caccgacacg
     6241 atggcgtata ccgcctctgc gctgtcgttc atgctggaga acctcggaaa accggttatt
     6301 gtgacagggt cacaaatccc gctggctgag ttacgctctg acggacaaat taatctgctg
     6361 aatgcgttgt acgttgcggc gaattatccg atcaacgaag taacgctctt tttcaataac
     6421 cgattgtatc gcggcaaccg cactaccaaa gcccatgccg atggttttga tgcgtttgcc
     6481 tctcctaacc ttcctccgtt actggaagca ggtatccata ttcgtcgttt gaatacgcca
     6541 cccgccccgc acggtgaagg ggaattaatc gttcatccaa tcaccccaca accaattggc
     6601 gtagtgacga tttatccggg gatttctgct gacgtcgtgc gcaattttct gcgccaaccg
     6661 gtgaaagcat tgattctgcg ctcctatggc gtgggtaatg cgccacaaaa caaagccttc
     6721 ctgcaggaat tacaagaagc cagcgatcgc ggtattgtgg tggtcaacct gacacaatgt
     6781 atgtccggta aagtgaatat gggtggttat gccaccggta acgccctcgc ccatgccggc
     6841 gttattggcg gtgcagatat gactgtagaa gccacgctaa ccaaactgca ttacctgctg
     6901 agccaggaac tggatactga aaccattcgc aaggccatga gccaaaacct gcgcggcgaa
     6961 ctgacgccgg atgattaagg agactgcaat gccccatcgc gccctgttac tggtcgattt
     7021 acaaaatgat ttctgtgctg gtggcgcgct cgccgtaccg gaaggtgaca gtacggtgga
     7081 ggttgctaac cgccttattg actggtgcca gtcgcgcgga gaagcggtta tcgccagtca
     7141 ggactggcac ccggcgaatc acggcagttt tgccagtcag cacggtgtag agccttatac
     7201 gccaggccaa ctcgacggtt tgccacaaac cttctggcca gatcactgtg tgcagaacag
     7261 tgaaggcgca caattacatc cgttactgaa ccaaaaagcg atcgcagcgg tgttccataa
     7321 aggcgaaaat cctttagttg acagttacag tgcctttttt gataacggcc gtcggcagaa
     7381 aacctctctc gatgactggt tacgcgatca tgaaatcgat gaattgatcg ttatgggcct
     7441 ggctactgac tattgcgtga agtttaccgt gctggacgcg ttacagttag gttataaggt
     7501 gaacgtgatt accgatggtt gtcgtggcgt gaatatccag ccccaggaca gtgcacacgc
     7561 gtttatggag atgtcagcag ctggggcaac gctatatacg ctggcagact gggaagagac
     7621 acaggggtaa ttttacgctg gcctacaatt ctgtactggc attgtaggcc aaataaaaca
     7681 cgtcagtggt acatctggca attgatgcca tcaacgaaag atttaattca cctcagaaat
     7741 ctcttccagc gacaccttcc gcgtttcgat gccaaaaatg gagagaacca gcgcacagag
     7801 cactaacatg acaccaagca ccataaacac cgtgatcgac ccataatgtg ttaataatgc
     7861 cgcaacgcca tagggcgtga aaactgcgac gatccgtccg acggcattaa cgaaacccga
     7921 accgcgcagg cgtaaatgcg ttggccaaag ctccgggata taaaccgctg acgcgaagca
     7981 aacatacatg tataaaaaga agatcatcac cagaccatag attaaaatcg cccactctgt
     8041 agtctgaatt gaatagatat agcctaacac ggcaataata ataagtaagg cggagccaaa
     8101 taatcgacga ggaaaatgat caataattaa tgccgcaata aatattccta ccggagcacc
     8161 aatcataata acagcggtca ttaatattga tttatcgaca tcaatcccgg agttaacaaa
     8221 tatggtcggt atccatacgg taatggtata aagcgaaatg ttcattgcaa ttaaaacagt
     8281 tatcgcaact aatgtacgtc gtaacatttc ccctttaaac aggagccaga aggtaccctt
     8341 gattacctta accttgctgt tgctctggtt caaagtaagc tggggtaaac gaatactctt
     8401 ctccctttca atttgctgct ctacttcacg aagttggctt tctgcaccgg cgatttgccc
     8461 tttccctgcc agccatcgtg gcgactcgat aaagtattta cctgagagaa accaggctaa
     8521 cagtatgcca ataccaccca gaagaaacat tattcgccaa ctaaaaaaag cgataaccac
     8581 cacgcctatc gccgcagaca gcatgggcga ccagttacca acaaatgaga gccgcgcgga
     8641 ccattttcca cgcaccgtcg cggggataaa ctccgtaaat gaggcatagc caaccataat
     8701 cagcgccccc attcctgttc ccatcaggaa gcgaaagaaa atgagccagt acatatcagg
     8761 gacaaaagcg gcccctgttg cagcaatacc gacgatgaga agatttatgc gaaacgccct
     8821 gcgccgccca aagtagtcac caataaaccc accagtaagt gagccgatga aataaccaaa
     8881 cattaatgcc gaggtaaagg cggcattgag gaaattattt gaccatccat tgcttaccag
     8941 ctttgctaag acgacattac ctgagtaact caaaaacccc gttaataaca gactaaagct
     9001 tattatacca aatatacgat aatgaaaccg ggccaaaggc aagcggtcca gtcttgcgcc
     9061 aatttgatca tattgttcca ttaggtgcct cggatttgct aaaaaggtag caattgcggc
     9121 aaattcactt gccgcatctt tatcatcatt ttatttcaag gcgcaggaac aaataatatt
     9181 gacaacgtgt gcttaaatta ccacaccagc tggatattat tgtctttgca aaatgctatc
     9241 cactctgcac ccggtgattt atcagttatt atgtaattaa tatgactaaa atcagctaac
     9301 tggacaaaag ctttgcgatc aaacttagag tgatcaacca ataacgcaac ttctgtcgcc
     9361 tgacggatca ttgtcttttt gatttcagct tccgcttcat tagagtccag cgcgccgctg
     9421 ttaatatcaa gacctttaca actcattacc atgatatcga catgatagcg gctgatgatc
     9481 tctttagtaa ttcttccttg cagtgaaagt gtatttttgt ttagttcccc acccgttgaa
     9541 acgactttaa tttctgactg agccagaaca tgaatcgctt ccgccgagtt ggttagcaac
     9601 gtcaggtcac tacggtcctg taacaatttg agcaattcca ttacggtact actggaatcg
     9661 gctgccatgg tggttttatt gtcgataaag ggtagtgcct tgcgtgcaat aagctgcttc
     9721 tcttcataaa acgatgaagc gcgcttataa aaatggatat tctccgtcaa catcgctgta
     9781 tttaaaacag caccaccata ggttctggtc aaaaagcctt catcttccag cttctcaaga
     9841 tcgcggcgaa tggtttcttc ggttacctga aaaatcccac tcaaatttga gactgtcact
     9901 tttttatcgt tggcaaccat ttgcttaatt gcctgaatcc tgtcttttgc cgccacgatt
     9961 acacccctgt atctttttac atcacattag cgcgattatc gcataaccga tgtttacttt
    10021 caaaacaacc tgtttgaatc acagattttc atcacagttt tcacagaaac agaggtgaat
    10081 cgtgttgagt atttaacgct ccagggcctc tgccatttcc ctcatcaatg ttgcgtctgc
    10141 atccgataag ttgataatca gtgccgcgac attttcgcgt acctgttccg gtgcagtagc
    10201 cccactaaga atggagatta aatcactctg ttttaatatc cacgccagtg ccagagtggg
    10261 aattgtgcac tgataacgag cacaaagtgg ctgccactgt tcaagcatat caatcacttt
    10321 cagcatgttt tcacgctgga accagacttt atttgcccga gcgccgcccg gaacgtaatc
    10381 acgagtgatg gtgccggtca acaatccctg ctctagcggg gaataaacct gaaccacaat
    10441 gccattatca cgacatagcg gcagcagttc gttttccatt gcccggtcga ggatactgta
    10501 tttcgcctga ataatatcca gttcaccata ttgcagatac tcgcggatat ggtcagcatc
    10561 gacgttagca gcgcctatag cgcgaatttt cccttcagcc ttcaactcat taagcactgc
    10621 gacagtttca gcgatcggcg taaaaaatgg cggcaccgac tgccagtgcg tcatgtagat
    10681 atcgatgtaa tcattaccca gacgttgcaa gctggcttct acctcttcgc ggatagattc
    10741 cggggaaagg tttttataca actgccgatc gccaactttg ttgaataaac ttccttttcg
    10801 ttcccagaca atgccgcatt tggtttctac tacaacctgt tcacggggca gtttttttaa
    10861 cgcctgaccg acgataactt cactattgcc aaagttatat cctggcgcag tatcaatcag
    10921 attaatgccg caacgatggg cttcaagaat cgtatcaata catatttgcc gatcgagatc
    10981 gccattccat gcaggaccgc cgccaatggc ccatgtcccc aaccccattc gcgaaagcgt
    11041 aatatccgtt gtgcctaaag gtatcttttt catttgcctt cattatcctt cgtattcttc
    11101 cagcaattgt tcgaccagct ttctgttttt tacgccggtg gtggcaccga cgcttagaac
    11161 cgagatagcc gccgttgcat tggcaaagcg tgcgcattca cgcagatttt tgccttctaa
    11221 cagtgccgca ataaaacctg aagcaaagtt atcgcccgcg ccaatggtgt cgatggcggt
    11281 tattcctgcg accgccggca ccttcatcgt catgtcacca cgcttgataa agcagccgtc
    11341 tttacccgtt ttaatcacca ccgtttttac gccgcacgca agaaagcagt cagcaatttc
    11401 atccaatgtc tctttcccgg tgagtaattt tgcctcggca taattaggaa acagataatc
    11461 gacataactc aatgcttcgc aaatatcatc cagcgtttca ttcaaccgcg gtttgatcat
    11521 atcggcacag ataatcatct gccgggtttt agcttgcgta aaaatttctg ttagcgcttt
    11581 accatccaat agaggactgt tgaaaatact gggcagagat aataatttcg cctgagaaaa
    11641 ccgcgaaaaa tcaacatcgt caatattcaa tttccacaga ctgccgttac ggtttgtgac
    11701 aaacgtccgc tcgccatcct ccgtcaccag tccaacgtta atagaggtat ctatgctgac
    11761 gtcctgcttc aggctttgaa tatcaatatt ctctttgcgg caatggtcga gaataaactg
    11821 tcctgcggca tctttaccaa tacggctcat taatgctgta cgatggccca gacgagaaat
    11881 aattgttgct tcatttattg catcaccccc ggtggtcatt gcgattcttt caagaggata
    11941 agaatccaca tcaaagatat ttttactgac cggttgcaat ggaatatcaa caatagcggc
    12001 acctatacaa ataacgtcga gattatccat atcattattc cgctttgcca tcagaaccga
    12061 acagtttaat tttttccaat gcccgttctt ttacagcttt acgtacttca cgttctaaat
    12121 gcaggaaagg ctgatcctgg ttttctttca ctgcgaccat tgcggcctga cagagctccg
    12181 tatgaatgtt gattttcgcg atacccaacg aaattgcagt tttaatgtcg gcatcactga
    12241 tccccgatgc accgtgcaaa accagcggaa cagaaacggc atcgcgtacg cgtttgacga
    12301 cctcaaagtt caattgtggc tctgacgtat aaaccccatg ctggttgccg atggcgacag
    12361 ccaatgagtc acagcccgta cgttcaacaa attcagccgc ctggtcagga tcggtatagt
    12421 gatagcccgc cagcgcttcc tcataaacgg tttcattacc gacatgccct aattccgcct
    12481 ctaccggaat acccagcgga tggaagaaat cgacagcctc tttggttaaa cgaatatttt
    12541 cttcgaaatc aaatgcagaa gcatcacgca ttaatgaatt cataccatga gtccaggcgt
    12601 tatgaataat ctccatactc cgaccatgat cccaatgagt tattaccggc acagttgctt
    12661 ttcgtgccat tgataccatc atgtgagaga aatcttcaaa tgaggtgttg ccgacaaaac
    12721 ctgtaccaaa agaaataata accggtgatt tcgcttcttc ggctgcgtcg ataacgccca
    12781 tcaacatttc tgcattccat acgttaaaat gggcaattgc ataatgttta tttgtggcat
    12841 cgttttccca atatctgata tctgcgagca tattctgttc tcccgtatta attaatcgct
    12901 aattttaata acgcctttaa taatgtcgcg tttgttgtta actgactctt caaatgcctg
    12961 ttgtacatcc cgataatcgt aaatatgcgt caccatcgat ttcacatcga atcgccctga
    13021 agaaatagct tcaatcgtga ccggataacg attggcatag cggaataccg tctggatagt
    13081 gacttcgcga ttgattttga ggaaattgat tgccgaatcg cccggtacag taccaacaat
    13141 cataatttta ccgccgcgca ttaccagata aggtgcctgt ttaatggtga ccgcagaacc
    13201 cgctgtttcg aaaacaatat ctgcgcccat gtcttcggta aattgctgac agcgtgcaat
    13261 agtgtcttct tttgtgccgt taataaccac tgtcgcacca agttgttccg ccattatcag
    13321 acgtttttcc agcacatcaa cgacggcaat ttccgttgcg cccaggcatt tgcacgcttg
    13381 caacgtcatc aaaccaatac aacctgctcc cagaataatt atcttcttac ccggtttaac
    13441 atctgccagc atcgcggcat gcatcccgac tgcggcaggc tccaccagcg ccccttccat
    13501 cgtgtccata ttgtctggca gtttgtaagt aaagctctcc ggatgacaca gatagtgcgt
    13561 taatgcgccg cggtagttgg gttgtgtcgc cataaaatca acgtccgggc agatgttata
    13621 tttgccttcc agacagtaac gacagtgacc gcaaggaacg ccaggttcga tatttacccg
    13681 atcccccggt ttaaatttgc gcacgcggct acccacagcc acaaccgtcc cggcgcattc
    13741 atgacccagg ccaatttctt gatttgggtc tttaggcgga ataaacgggc ctgattcaaa
    13801 accatgtaca tctgaaccac aaataccgac atattctact ttaatcaaaa cttcatcttc
    13861 tttaggcact ggtatttctg ctgaaataat tttcattgtg cccggcacct gcaatattgc
    13921 ttttgaattt ttcattttga atccacccgt attatttatt ggctactgca tcaatactta
    13981 aactttcaac ggagacacct ttggtttcaa ttccaatggt ggcgatagca attgcgacaa
    14041 caattgaaac agcccccaga agaataaaga ctcccgttac gccataacta ctgagcagca
    14101 ctgcaacggc ataaggtgcg gcaataccac tgattcgccc taccgcattc gccagaccgg
    14161 agccacggag tttggcctct gtcggccaga tttcagggac atacactgcc gaggcatagc
    14221 aaacgtacat atagacgaaa gtaatcagga agaaaccaat caacgttatg agcaacatac
    14281 tggtttgcag tgaatagata tatccgagca ccgcaatcag gattaatagc cccacaccca
    14341 ttgttttacg cggaatttta tccatcacca gcatggcaat aaaaatacca aatggcgcgc
    14401 caaacatact catggtattt aaaacaatcg agtcttttaa attaatcccc tgggtcatga
    14461 atattgttgg caaccagtta attagtgtgt actgcacaac gttcatggca atcagcacac
    14521 aagagcctaa tatcacgcgt ttcagtaata ctcctgtcag taaggctgaa tacggcaccg
    14581 cttgtggcgc tttaccgtca tcagcaataa ccacaggcgg caaaggttta cccgtctggc
    14641 gtatgacgcc ttcttctata ctccgcatga ctttctcggc ttcctgatac cgtccgcgcg
    14701 attccagcca gcgcggggat tcaggaaagt agcgccaggc cagcgccgta gcgataagcg
    14761 acaatattgc agggataagc agttgtaccc gccagttcca ctctgcacta atcagcggcg
    14821 tgagtcccat cgctatcaat gaacagagcg gatatgacca gttgccaata aaggaaaccc
    14881 gacttgacca cgttccacga tttctaccgg gcatatattc ggtgaaacca gcaaacagcg
    14941 taaccagtaa agctcccagc ccaactccca tcacaaaacg gcaggcaata aggaaatcca
    15001 tattcggtga gaaagcaccg acaaccatcg aggcaatatg aatggcctca tagaggatga
    15061 acgcatttct gcgaccggtt ttgtcgccaa tgatgccgcc caccagcgca ccgagaaaca
    15121 ttccggcggt cgtgattgct gagaatgtgg ctgtggtgga attatctgtc cagcccaacg
    15181 ctttcagctg cgcgaggatc aagccaccaa cggcattact ccagcagaca agcaagccaa
    15241 acgcgacgat ggcaaacatt gacgaatgcc agcggcaatc cggtaagcga tccagccgtg
    15301 caccacaatg cggttttgtt atctgttcca tgcgataacg ttccttcagg agagtggtta
    15361 ttcatcaaag tcgtaagtca tgatcacttt gattgcggtt ttatcgacca tcgcatcaaa
    15421 cccttcgcgc cattgcgaca ggccgatacg atgcgtgatc atcggtttga ctttgatagc
    15481 gccgctggct aatagccgga tagcgttacg ccatgaggtg gagtcatagg ccatatgccc
    15541 aatgatgctt ttattccagg cggtaatgtc attaatcgag aaatcaagag gtttgaagcc
    15601 cattccaacg cgtaccactt ccccattcgg gcggagcatt tcgatggctt gtttcagtgc
    15661 gatattggca ccggagcatt caatcaccag tcccagatta tctttgccac aaatttgctg
    15721 gcagcgcgcc accacatctt cggtagaacc atttactact gccgtcgccc ccagttcttt
    15781 tgcaaccggg aagcggaccg ccacatcttc ttgcagacca acgacgacga tatttaccgc
    15841 ccccataatc cgcgccattt gtacggaaaa cagcccgagt gggccagtgc cgatgacgac
    15901 aacatcctga ccaggaagga atttcgattg ctgcgcgatg gatttatagg cattacagat
    15961 agggtcaagt acggctgcgt cctcataatc aacaccatca gggatttccc acaacgcatg
    16021 acgatgaatt ttgagaattt caccaggaac cagacaatat ttggaaaaac caccgcccca
    16081 ggtattgtta tccagaccaa ggtttacttt ttctgtacaa cacagaaaat caccctgttc
    16141 acaggccgga caaacgccgc aaacgtgacc gctgttatcc gacacgacgc gttgccccac
    16201 tttccagtct ttgacttttt caccaacctg cgcaatacaa cctgcgaact catggccgcg
    16261 gatagagtta aactcatcag aaccgctatc gacattgtag tgcttcatgt ctgcgccgca
    16321 gattgccgcg gctttaattt caatcactac gtcttccggg ccacaaatgg gttgtgggac
    16381 atcaatcatc ttgtagccgc caaaggcctt gccaaaccga gccagtgctt tcattgcgtc
    16441 atcctctgtg ttttgttttg cttagatggc catcatactt acccacatga tttttgaggt
    16501 caacattaaa tctgtaaaac acagataatg atctgcgttt tacaacttag ttcacaatta
    16561 cgcagaaaat ggctgggtct gttacaggtt ggtggaaggc gggacgcaaa aagagcaaat
    16621 tcgagaggga ttaccggcca aataggtctg agacttaagt tcaggagaag tcattcaggc
    16681 gtgaacgcga ctgcaagaga tcggactggg acttagccag gtcgggtcga gataattgag
    16741 acgagtcgtg gggtcattgt tggctatccc cttcatagcc gcggcaaaaa ggggatgaac
    16801 cacttactta aacatagcaa taggctttgc tgagatacca aaatcttctt ttaactgctg
    16861 tttgctcttc atgaccatct gaccatttgt gtcaattgtc atatgctgtg cttcggtatt
    16921 atggcgagct tgccacagca tcaccagttg caggcagttc gctttttgtt cctcggtcaa
    16981 cgcaacgcca tcgggccatt tccccagttc aacggcggtc gacaaacgct ggtatacctc
    17041 aggcgtcatg ctgttgataa tgtcatcaag attcataatt tttccgctcc tgtggaataa
    17101 gttgctgaat cgttttttca accgttgatt tcttcgccgt tttcgccatc ggtaaagcgt
    17161 aaagaggcag agttaacaca ataacgttcg cccgttggct gcggcccgtc agggaagaca
    17221 tgccccagat gggcatcaca gttaccgcaa cgaatttcta tgcgctgcat tccatgtgac
    17281 aagtctttga tataacgaat ggattcttca ctcaccggtt cgtagaaact gggccaacca
    17341 cagccggaat catacttggt ttgggaatga aacagcgggg catcgcagat caaacagtga
    17401 tatacgccgt cacgcttgtt atgcagtaaa cgacccgtaa atggcggctc tgtcccatga
    17461 ttctgcgtca cgtaaaactg catctcggac aaattttttt tcagttcttc tgccgaaggt
    17521 ttattagcca tttgctcaca tctcacttta atcgtgctca cattacgtga ctgattctaa
    17581 caaaacatta acaccaactg gcaaaatttt gtcctaaact tgatctcgac gaaatggctg
    17641 cacctaaatc gtgatgaaga tcacattttt atcgtaattg ccctttaaaa ttcggggcgc
    17701 cgaccccatg tggtctcaag cccaaaggaa gagtgaggcg agtcagtcgc gtaatgctta
    17761 ggcacaggat tgatttgtcg caatgattga cacgattccg cttgacgctg cgtaaggttt
    17821 ttgtaatttt acaggcaacc ttttattcac taacaaatag ctggtggaat atatgactat
    17881 caaagtaggt atcaacggtt ttggccgtat cggtcgcatt gttttccgtg ctgctcagaa
    17941 acgttctgac atcgagatcg ttgcaatcaa cgacctgtta gacgctgatt acatggcata
    18001 catgctgaaa tatgactcca ctcacggccg tttcgacggt accgttgaag tgaaagacgg
    18061 tcatctgatc gttaacggta aaaaaatccg tgttaccgct gaacgtgatc cggctaacct
    18121 gaaatgggac gaagttggtg ttgacgttgt cgctgaagca actggtctgt tcctgactga
    18181 cgaaactgct cgtaaacaca tcaccgctgg tgcgaagaaa gtggttatga ctggtccgtc
    18241 taaagacaac actccgatgt tcgttaaagg cgctaacttc gacaaatatg ctggccagga
    18301 catcgtttcc aacgcttcct gcaccaccaa ctgcctggct ccgctggcta aagttatcaa
    18361 cgataacttc ggcatcatcg aaggtctgat gaccaccgtt cacgctacta ccgctactca
    18421 gaaaaccgtt gatggcccgt ctcacaaaga ctggcgcggc ggccgcggcg cttcccagaa
    18481 catcatcccg tcctctaccg gtgctgctaa agctgtaggt aaagtactgc cagaactgaa
    18541 tggcaaactg actggtatgg cgttccgcgt tccgaccccg aacgtatccg tagttgacct
    18601 gaccgttcgt ctggaaaaag ctgcaactta cgagcagatc aaagctgccg ttaaagctgc
    18661 tgctgaaggc gaaatgaaag gcgttctggg ctacaccgaa gatgacgtag tatctaccga
    18721 tttcaacggc gaagtttgca cttccgtgtt cgatgctaaa gctggtatcg ctctgaacga
    18781 caacttcgtg aaactggtat cctggtacga caacgaaacc ggttactcca acaaagttct
    18841 ggacctgatc gctcacatct ccaaataagt tgagatgaca ctgtgatcta aaaagagcga
    18901 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
    18961 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
    19021 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
    19081 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
    19141 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
    19201 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
    19261 acgctgaaat cacatcgtga agatgctgat ggcgtagcgc tgacttttga attgacacaa
    19321 agcgaagaga cgaaaaaatt ctggccgcac gactttacgc tgttagcgca tttccgcgtg
    19381 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
    19441 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
    19501 ttcattgata aagtaaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
    19561 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
    19621 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt cggctggaac
    19681 ccgggaccgg cgctttcagt tagcatgggc gacatgccgg atgatggcta caaaacattt
    19741 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tgaccaaaga gaaacctgca
    19801 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
    19861 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
    19921 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
    19981 gccgcattct gttggacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
    20041 tggctgatta cccacgccaa caatacttgt gccgcgctga tattgtgagc atgtgcaatt
    20101 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
    20161 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
    20221 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
    20281 tgccagagtt cctgcatatc agcatgatca aggttagaaa cgccccagcg gcggattttt
    20341 ccctgggcga tcaatttttc catcgctgcc acagtctctt caaaagcgaa actgccagac
    20401 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
    20461 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
    20521 ttatcgcgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
    20581 atttcggcgg tatcaatgag ggttaaaccg agttcaatgc ccgcgcgtag tgcagcaact
    20641 tctgttttgc gctgactggc atcttcgcca atataccatg ttccctgccc tatggctggc
    20701 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
    20761 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
    20821 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
    20881 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
    20941 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
    21001 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
    21061 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
    21121 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
    21181 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
    21241 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
    21301 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
    21361 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
    21421 tgattaccgg tactgggtaa acatcagtat cgtaatcttt atatgggtgc tcaacgacac
    21481 ctacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
    21541 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
    21601 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
    21661 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
    21721 gaatttagcg ttactggcta ccctggtctt tacattaatt atgcaaaatt tatggatgag
    21781 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
    21841 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
    21901 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
    21961 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
    22021 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
    22081 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
    22141 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
    22201 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
    22261 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
    22321 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
    22381 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
    22441 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgctcaga ttctggaaaa
    22501 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccatggg cggcaaaacg
    22561 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
    22621 tctgcaacaa attgctatcg ccaaaacgga acccggtgat gagaacaacc aggacatctc
    22681 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
    22741 cgcctacggc tattccggtg cgctgtgccg cgccaaccaa gggatcatgg aatttgtcga
    22801 gatgtttaaa gcaccgataa aagtgctgca tccgctgtta accgccactc aggaaggtaa
    22861 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
    22921 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
    22981 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
    23041 gaaattgctt aatcacagtg aattgactca cgccccctgc gcccctggca cgctggaaac
    23101 gctgtcacgt ttttccattc tttcgcgcct taaagagcca gaaaactcca gcatttattc
    23161 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
    23221 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
    23281 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
    23341 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cacaagagca
    23401 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
    23461 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
    23521 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
    23581 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
    23641 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
    23701 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
    23761 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
    23821 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
    23881 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
    23941 gtcttaacaa ccctggcccg gtacacatgt taccgggcct acaacgacag cgaaccgtgg
    24001 gcctgagaag cggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
    24061 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
    24121 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
    24181 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
    24241 aaccgatgtt tcatcagggg cgtggcggtc tgcgccaccg cgtgcatccg ggcaatgacc
    24301 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggtagtg
    24361 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
    24421 aagatgagta tcttgatttg ctctttgaag atttggcctt accgaatctg aaacaaaacc
    24481 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
    24541 cggcaaatat cagcgttgtg cgctccttgc agaactcact ggcgcgacgc acagccatga
    24601 cggcaggcaa gcggcgggaa cttcatgcac tggaagagaa tttggccatc atcagcaaca
    24661 gtgaacctgc gcagctgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
    24721 ccaaaattga acgcgtccct tttattgata ccttcgattt acgttacaag aactacgaga
    24781 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
    24841 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
    24901 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
    24961 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
    25021 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
    25081 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
    25141 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
    25201 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
    25261 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
    25321 aaaattcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
    25381 agcttactgt
//
LOCUS       DAPHJE010000064        25118 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPHJE010000064 DAPHJE010000000
VERSION     DAPHJE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.064"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(2..77)
                     /locus_tag="RH222_002646"
     tRNA            complement(2..77)
                     /locus_tag="RH222_002646"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Val,seq:tac)
     gene            complement(122..197)
                     /locus_tag="RH222_002647"
     tRNA            complement(122..197)
                     /locus_tag="RH222_002647"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(162..164),aa:Val,seq:tac)
     gene            456..1871
                     /gene="gltX"
                     /locus_tag="RH222_002648"
     CDS             456..1871
                     /gene="gltX"
                     /locus_tag="RH222_002648"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV3797648.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1923..2315)
                     /locus_tag="RH222_002649"
     CDS             complement(1923..2315)
                     /locus_tag="RH222_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV3797649.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2317..2676)
                     /locus_tag="RH222_002650"
     CDS             complement(2317..2676)
                     /locus_tag="RH222_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV3797650.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            2897..2972
                     /locus_tag="RH222_002651"
     tRNA            2897..2972
                     /locus_tag="RH222_002651"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2930..2932,aa:Ala,seq:ggc)
     gene            3012..3087
                     /locus_tag="RH222_002652"
     tRNA            3012..3087
                     /locus_tag="RH222_002652"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3045..3047,aa:Ala,seq:ggc)
     gene            3295..5484
                     /locus_tag="RH222_002653"
     CDS             3295..5484
                     /locus_tag="RH222_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV3797651.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGGETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5534..6736)
                     /gene="nupC"
                     /locus_tag="RH222_002654"
     CDS             complement(5534..6736)
                     /gene="nupC"
                     /locus_tag="RH222_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV3797652.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            7072..8310
                     /locus_tag="RH222_002655"
     CDS             7072..8310
                     /locus_tag="RH222_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV3797653.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8451..8777)
                     /locus_tag="RH222_002656"
     CDS             complement(8451..8777)
                     /locus_tag="RH222_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV3797654.1"
                     /translation="MFRSLFLAAALMAFTPLVANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8892..10148)
                     /locus_tag="RH222_002657"
     CDS             complement(8892..10148)
                     /locus_tag="RH222_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV3797655.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHSMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10352..11317
                     /gene="glk"
                     /locus_tag="RH222_002658"
     CDS             10352..11317
                     /gene="glk"
                     /locus_tag="RH222_002658"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV3797656.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            11536..11862
                     /locus_tag="RH222_002659"
     CDS             11536..11862
                     /locus_tag="RH222_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDV3797657.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            11884..13131
                     /locus_tag="RH222_002660"
     CDS             11884..13131
                     /locus_tag="RH222_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV3797658.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            13146..14231
                     /gene="ypdF"
                     /locus_tag="RH222_002661"
     CDS             13146..14231
                     /gene="ypdF"
                     /locus_tag="RH222_002661"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3797659.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            14231..15268
                     /gene="ypdE"
                     /locus_tag="RH222_002662"
     CDS             14231..15268
                     /gene="ypdE"
                     /locus_tag="RH222_002662"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3797660.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15293..17788
                     /gene="ptsP"
                     /locus_tag="RH222_002663"
     CDS             15293..17788
                     /gene="ptsP"
                     /locus_tag="RH222_002663"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3797661.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(17791..18647)
                     /locus_tag="RH222_002664"
                     /pseudo
     CDS             complement(17791..18647)
                     /locus_tag="RH222_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(18660..19394)
                     /gene="ypdB"
                     /locus_tag="RH222_002665"
     CDS             complement(18660..19394)
                     /gene="ypdB"
                     /locus_tag="RH222_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV3797662.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19409..21106)
                     /gene="ypdA"
                     /locus_tag="RH222_002666"
     CDS             complement(19409..21106)
                     /gene="ypdA"
                     /locus_tag="RH222_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV3797663.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21483..22721
                     /gene="alaC"
                     /locus_tag="RH222_002667"
     CDS             21483..22721
                     /gene="alaC"
                     /locus_tag="RH222_002667"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV3797664.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(22786..22857)
                     /gene="ypdK"
                     /locus_tag="RH222_002668"
     CDS             complement(22786..22857)
                     /gene="ypdK"
                     /locus_tag="RH222_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV3797665.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(23213..24133)
                     /gene="lpxP"
                     /locus_tag="RH222_002669"
     CDS             complement(23213..24133)
                     /gene="lpxP"
                     /locus_tag="RH222_002669"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV3797666.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            24486..24728
                     /locus_tag="RH222_002670"
     CDS             24486..24728
                     /locus_tag="RH222_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV3797667.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(24805..25080)
                     /gene="ypdI"
                     /locus_tag="RH222_002671"
     CDS             complement(24805..25080)
                     /gene="ypdI"
                     /locus_tag="RH222_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HDV3797668.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
BASE COUNT         6031 a         6178 c         6648 g         6261 t
ORIGIN      
        1 gtggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
       61 agctgagcta atcacccact tctcaatttc ttgctacacg gcggagacta cataaagtag
      121 ttggtgggtg atgacgggat cgaaccgccg accccctcct tgtaagggag gtgctctccc
      181 agctgagcta atcacccccg ctgtgtggag tcgcattata gggagagttc aaaatgagtc
      241 aacgcatttt ctaaagaaat tgttcgttcg tcgtaaattt aagcaagatg atcgcaaaac
      301 agaccgtgtt gcgcaatttg tcaacgaaaa caataatgcg taaggttgca acccgaacta
      361 cattgaggaa tcaggcggga gtgatagaat atcgcccact caatttttcc aggatttgcc
      421 ggttgtcggc atctttctaa acgtaaggcc atttcatgaa aatcaaaact cgcttcgcgc
      481 caagcccaac aggctatctg cacgttggcg gcgcgcgtac tgctctttac tcctggcttt
      541 ttgcacgtaa ccacggcggt gagttcgtgc tgcgtattga agacaccgat cttgagcgtt
      601 ccacgccgga agctatcgaa gccattatgg atggcatgaa ctggctgagc ctggagtggg
      661 atgaaggtcc gtactaccag accaaacgtt ttgatcgcta caatgcggtg atcgatcaga
      721 tgctggaaga gggcactgct tataaatgct attgctctaa agagcgcctg gaagcgctgc
      781 gcgaagagca aatggcgaaa ggtgagaagc cgcgttatga cggtcgctgc cgccacagcc
      841 atgagcatca tgctgatgat gaaccgtgtg tcgtgcgttt tgctaatccg caggaaggtt
      901 ctgttgtttt tgacgatcag atccgtggtc cgatcgagtt cagcaaccag gagctggatg
      961 atctgattat ccgccgtacc gatggttccc caacctataa cttctgtgtg gttgtcgatg
     1021 actgggatat ggaaatcacc cacgttatcc gtggcgaaga ccatatcaac aacacgccgc
     1081 gccagatcaa cattcttaag gccctgaaag cgccggtgcc ggtttacgcg cacgtttcta
     1141 tgatcaatgg cgatgacggt aaaaaactgt ccaaacgtca cggggcagtc agcgtaatgc
     1201 agtatcgtga tgacggttat ttgccagaag cactgctgaa ctatctggtg cgtctgggct
     1261 ggtcccacgg cgatcaggaa atcttcactc gtgaagagat gatcaaatac ttcactttga
     1321 atgccgtcag caaatctgcc agtgcgttca acaccgacaa gctgctgtgg ctgaaccatc
     1381 actacattaa cgcgctgccg ccggagtatg ttgctactca cttacagtgg cacattgagc
     1441 aggaaaatat cgatacccgt aacggcccgc agctggctga tctggtgaaa ctgctgggcg
     1501 aacgctgcaa gacgctgaaa gagatggcac agagctgccg ttatttctac gaagattttg
     1561 ctgagttcga tgccgatgcg gcgaaaaaac atctgcgtcc ggtagcgcgt cagccgctgg
     1621 aagtggttcg tgacaaactg gccgcgatta ctgactggac cgctgaaaac gttcatcacg
     1681 ctattcaggc gacggcggat gagctggaag tgggtatggg taaagttggt atgccgctgc
     1741 gtgtcgccgt aaccggtgcg gggcagtctc cagcactgga tgttaccgtt cacgcaattg
     1801 gtaagacccg cagtatcgag cgtatcaaca aagcgctgga ttttattgct gaacgcgaaa
     1861 atcagcagta attagcgtgt aaaaagataa acggcaggag ataatatcct gccgtttttt
     1921 atttatgctg attcgtcaat atcgagacgg gcgaggaaat tgcgaattcc ttcacgtgac
     1981 aaaaattgcg cgactttttc ctgctcaatg tctgtcatat tatctatcgc gttgatgatt
     2041 tgtcgatacg ccggggcgcg tgctccagga gcgtattcag caattctctc ttcagcttcc
     2101 atcaacattg gtgtgttatg gaaggctggc gtgttctgaa taaactcgca aacttgcgta
     2161 tcgaccagaa taagtctggc acgaccgcct ttcacgccgg gaaatttttc cgttttccag
     2221 cctttttctc tgatccaacg gttaacagtt tgtttagcca caccgaggca ttcagccagt
     2281 tcttcggtgg tcattttgtt gcgtaatctt ttcatattat ttgctatcgc gaatccctaa
     2341 gcgttgtaac aatccgataa tcccttcccg cagaagcaag gatgtaaact gtttttgttc
     2401 aactggcgtc atctctttcg ccagtgtcac cagcaatact tcaagcggcg catcaccaga
     2461 aagggcaggc gcttctccct gaccttctgg acgttcagca ttgcgaatat attcacgaac
     2521 ctgttcattg acgtgaacca gtcgggcttt gccaccctgg acgccaggtt ttggtgacgt
     2581 tgtccagcct tccttgcgta cccatttatt aatggtctgg cggctatagc cggtcagtct
     2641 ggcaagttca tctggtgtca tcctctcctt gaacataaca attccctgaa tgtatgtggg
     2701 tctattagta gttcatttta taacaccact ttacaggaaa ccgtgacatg aataactact
     2761 gaatgcgagc ggttttccaa aattgttcat ttgcctgatt tttcagcgat tgaaattaca
     2821 cgttgcaatt tgccgttgac acattcgggc ggaattcata tgatgccgcc cgtcaactcg
     2881 acaagcttta cgtgacgggg ctatagctca gctgggagag cgcttgcatg gcatgcaaga
     2941 ggtcagcggt tcgatcccgc ttagctccac caaattttgc acccagcaaa tttggtacgt
     3001 aaacgcatcg tggggctata gctcagctgg gagagcgctt gcatggcatg caagaggtca
     3061 gcggttcgat cccgcttagc tccaccaaat ttccaaccct cgctgcaaag cgggggtttt
     3121 ttgtctctgc tttttgccgc ttttgtaata cagtctacgt ccgggttagc gccgcctggt
     3181 gaaagcatca ttggatgaaa aatcggcaac aggctggccc ctgtttgctt cgcgatgcga
     3241 ataaacttat tatttgtgtg cctgaaaacc ccgatcagtg agagtagtgt actcatgttt
     3301 gtggagcata acctgataaa aaatatcaag atattcacac tagcgtttac gctcaccgtg
     3361 gtacttattc agctatcccg ttttatttcg ccacttgcca ttatccattc cagttatatc
     3421 tttctggcgt ggatgccact gtgcgtaatg ctgtcaatct tgtttatctt tggctggcgc
     3481 ggtgtcgttc ccgttttatg cgggatgttt tgcaccaatc tgtggaactt tcatctctct
     3541 tttttacaga ctgcggtcat gctcggtagc cagacgtttg tcgtgttgtg tgcctgcgca
     3601 atattacgct ggcagctggg gacgcgttgg cgttatggat tgaccagccg atatgtctgg
     3661 caacgtctgt tctggcttgg tttggtgacg ccgatcggca tcaaatgcag catgtatctt
     3721 gtggggagct tctttgattt cccgctaaag atatccacct ttttcggcga tgcggatgcc
     3781 attttcacgg tcgttgattt gctaagcctt ttcaccgctg tgctgattta caacatgctt
     3841 ttctactatc tcactcgcat gattgtaagt ccccactttg cgcagatatt gtggcgcagg
     3901 gatatcgcgc cgtcgttggg caaagagaaa cgcgcattta ccttaagctg gctggcagcg
     3961 ttaagcgtgc ttctacttct gttgtgcaca ccttatgaaa acgactttat tgccggttac
     4021 ctggtacccg ttttcttcat catctttacc ctcggggtcg gtaagcttcg ctatccgttt
     4081 ttaaatctca cctgggctgt ttcaacgctt tgccttctga attacaacca gaactttttg
     4141 caaggggggg aaaccgaata ttcgctggca tttattcttg cggtgctgat ttcctttagc
     4201 gtttgcctgc tctatatggt gcgcatttat catcgcagtg aatggcttaa tcgccgctgg
     4261 catttgcagg cgctgacaga tccgttaacg ctcctaccca actttcgtgc gttggaacaa
     4321 gcgccggaac aagaggcggg caagagtttt tgctgcctgc gcattgataa tcttgagttt
     4381 atgagtcgtc attacggctt aatgatgcgc gttcactgta tccgctcaat ttgccgtacg
     4441 ctgctgccgt tgatgcagga aaacgaaaag ttgtatcaat tgccgggtag tgaactgctg
     4501 ttagtgctga gcgggccgga aacggaaggg cgactccagc atatgattaa catcctgaat
     4561 agtcggcaaa ttcactggaa caataccggg ctggatatgg gctatggtgc tgcctggggg
     4621 cgttttgatg gaaatcagga aaccctgcaa cccttgttgg ggcagttaag ctggctggcg
     4681 gagcaatcct gcgcacatca tcatgtgctg gcgctggata gcagagagga gatggtttcc
     4741 gggcagacca ctaaacaggt gctattgctg aataccattc gcacggcgtt agatcagggt
     4801 gatttgctgc tctacgccca gccaattcgc aacaaagagg gtgaaggtta tgatgagatc
     4861 ctcgcgcgac tgaaatatga cggcggcatt atgaccccgg ataagtttct gccccttatt
     4921 gctcagttta accttagcgc gcgttttgat ttgcaagtgc tggaatcctt gttgaagtgg
     4981 ctggcaacac acccttgcga caaaaaagga ccgcgctttt cagtcaattt aatgccgctc
     5041 acgctgctgc aaaagaatat tgccgggcgg attattcgtc tgtttaagcg ttatcacatc
     5101 tccccgcagg cggtcattct tgagatcacc gaggagcagg cgttttctaa cgcagaaagc
     5161 agcatgtaca acatcgagca gctgcataag tttggtttcc ggattgcgat tgatgacttt
     5221 ggcaccggat atgccaacta cgaacggtta aagcgtttgc aggctgatat catcaaaatt
     5281 gatggcgtct ttgtgaaaga tattgtcacg aacacgctgg atgcgatgat tgtgagatca
     5341 attaccgatc tggcgaaagc gaagtcattg agtgtggtcg cggagtttgt cgagacgcaa
     5401 cagcagcagg cgctattgca taagctcggg gtgcaatatc tgcaagggta tttgattggt
     5461 cgcccgcagc cattagctga ttaacaggcg taaaaaaacc ggggaattat ccccggcttt
     5521 tttatgtatg gtcttacagc accagtgctg cgattgacgc agacagcaca ctcaccaggg
     5581 tagagccgta aaccagcttc agaccgaagc gagaaaccac gttaccttgc tcttcattca
     5641 ggcctttaac tgcacctgcg ataatcccga ttgaagagaa gttagcgaag gaaaccagga
     5701 acacagagat gatgccttca gcacgcggag agagcgtgga agcaattttc tgcagatcca
     5761 tcatcgcaac gaactcgttg gaaaccagtt tggtcgccat gatactgccc acttgcagtg
     5821 cttcactgga aggaacaccc atcacccatg caatcggata gaagatgtag cccaggatgc
     5881 cctggaagga gatgctgtag ccaaaccagc cagtaacggt ggcaaacagt gcgttcagcg
     5941 cggcgatcag ggcgataaag ccgatcagca tcgcggcaac gataatggca actttgaaac
     6001 ctgccagaat gtattcaccc agcatttcga agaagctctg accttcgtgc aggttggaca
     6061 tctggatgtt ttcttcactg gcatcaacac ggtaaggatt gatcagcgac agcacgataa
     6121 aggtgctgaa catgttcagt accagcgcag caacgacgta tttcggttcc agcatggtca
     6181 tgtatgcacc aacgatggac atcgacacgg tggacattgc cgtggcagcc atggtgtaca
     6241 tacgattacg ggagattttg ccgaggatat ctttataggc aataaagttt tcagactgac
     6301 ccagaatcag ggagctgacg gcgttaaagg attccagttt gcccatgccg ttgactttgg
     6361 agagcaggaa accaattgcg cggatgatca ccggcaacac gcgaatgtgc tggagaatac
     6421 cgatcagtgc agagataaag acgattgggc acagcacttt caggaagaag aatgccaggc
     6481 cttgatcatt catgctacca aagacgaagt tagtcccttc gttggcaaat ccgagcagtt
     6541 tttcgaacat ttcggagaag cctttcacga agcctaaacc aacgtcggag ttcaggaaga
     6601 accacgccag taacacttcg ataacaagca gttgaataac ataacggata cgaatttttt
     6661 tgcggtcgct gcttaccagc agtgcgagaa tcgcaacaac ggcaagtgcc agtacaaaat
     6721 gaaggacgcg gtccatattt gctccaaata tgaggcaggt taaatttccg tgcacattct
     6781 atgtaacatg gttaaggaaa acgagatctg acacacacta taatgacgtc ctgtgacaag
     6841 cttcaaaaat agtgatctgt catacatttc tgctatgttg tgtatggaag ctgaaagtta
     6901 tgtaaatgtg ctaacattaa acataaccat aacattcaag tcatcaatct tttcgtagtc
     6961 ataatcctgg tctatcaaag aaatcaccac aacccattca aatgaatttg ataatcattc
     7021 tcgtttggca tagcatgaaa catagcaaag gctatgtttt agaggcacaa gatgacgaac
     7081 tatcgcgttg agagtagcag cggacgggcg gcgcgcaaga tgaggctcgc attaatggga
     7141 cctgcgttca ttgcggcgat tggttatatc gatcccggta actttgcgac caatattcag
     7201 gcgggtgcca gcttcggcta tcagctactg tgggttgtcg tttgggccaa cctgatggcg
     7261 atgctgattc agatcctctc tgccaaacta gggattgcca ccggtaaaaa tctggcggag
     7321 cagattcgtg atcactatcc gcgtcccgta gtgtggttct attgggttca ggcagaaatt
     7381 attgcgatgg caaccgacct ggcggaattt attggtgcgg cgatcggttt taaactcatt
     7441 cttggtgttt cgttgttgca gggcgcggtg ctgacgggga tcgcgacttt cctgatttta
     7501 atgctgcaac gtcgcgggca aaaaccgctg gagaaagtga ttggcgggtt actgttgttt
     7561 gttgccgcgg cttacattgt cgagttgatt ttctcccagc ctaacctggc gcagctgggt
     7621 aaaggaatgg tgatcccgag tttacctact tcggaggcgg tcttcctggc agcaggcgtg
     7681 ttaggggcga cgattatgcc gcatgtgatt tatttgcact cttcgctcac tcagcattta
     7741 catggcggtt cgcgtcaaca acgttattcc gccaccaaat gggatgtggc tatcgccatg
     7801 acgattgccg gttttgtcaa tctggcgatg atggctacag ctgcggcggc gttccacttt
     7861 tccggtcata ctggtgttgc cgatcttgat gaggcatatc tgacgctgca accgttgtta
     7921 agccatgctg cggcaacggt ctttggatta agcctggttg ctgccggact gtcctcaacg
     7981 gtggtgggga cactggcggg gcaggtggtg atgcaggggt tcattcgctt ccatatcccg
     8041 ctgtgggtgc gtcgtacagt caccatgttg ccgtcattta ttgtcattct gatgggatta
     8101 gatccgacac ggattctggt tatgagtcag gtgctgttaa gttttggtat cgccctggcg
     8161 ctggttccac tgctgatttt caccagtgac agcaagttga tgggcgatct ggtgaacagc
     8221 aaacgcgtaa aacagacagg ctgggtgatt gtagtgctgg tagtggcgct gaatatctgg
     8281 ttgctggtag gaacggcgct gggattgtag ttgaatgagc gtcgcatctg gcactattgg
     8341 cggatgcggc gtaaacgcct tatccgccct acgcgttaaa gagccggttt gtaggcctga
     8401 taagacgcgc tagcgtcgca tcaggcattg tgctccattg gcgaatgtgt ttaatggcgg
     8461 tgtccatgtc cgcggccttt tccgcgatga tcgtcacgat cgcgccagcc ttcacgatag
     8521 ccacgctcat acgctttacg cttatcccag ccacgatgat agccattatc atgacgccac
     8581 cagcggtttt tacgccattc ataattgcga tgccagtagt cacggtcgcg ccagtgacca
     8641 ccgtcccagt aattaccgta atgatcgcga tcgccaattt gtaatttgat tgatggcagt
     8701 agggtgattt cacctgcgtt tgctacaagc ggggtaaatg ccatcagggc ggccgccaga
     8761 aacagtgacc tgaacattgt tattctcctt cacgatcgaa gccgtcagcg gcctgttaac
     8821 gcaatattac ggggaggtaa agccccgtat catcgccata actcttaaat cataaaggag
     8881 agcatttttt gctaaacgtt atcgtggggt ggctgttgtt tcggacgatt gaccatcatc
     8941 atcggcttac ctgctaataa cagccatgcc ggaagcatga cgatacagag cagcggtagc
     9001 aatgtggtat tgggtacaac gactgccgcc ataaaaagac ttaaccagcc atcgcgtgtt
     9061 accaccagca cgatgccgag aatagcgcaa gaaacggtta ttgccgctgg tacggcggga
     9121 acgtgctcat gcagcatcaa ccctaatgcc acgccgacaa acactgccgg gaagattcgc
     9181 ccaccgcgaa agccactggc ggcagcaacg accagggcgg caagtttaat taccgccagc
     9241 aaaaagtaat cgctggtgct gaaagcctga tttgccacca tctgctgcat ctcatccagc
     9301 cctttaaaca gcgaaactgg tccaccaata acccccagaa taccgagaat aaatccgcca
     9361 atacccagca cgagcaccgg atttttcatt tgatgcatca ttgagtgcaa ccgtggtaag
     9421 caccatacgg cgaccatccc tgctgcgatg gcaatcgccg cgacaattgc accgctgaga
     9481 atatcggtca tttccatctg tccgtaatgc gcaatgggca gtgaaaaatg aggatggaaa
     9541 aataatccgg tagtaagtgc accagctgct gccgccatta acggcgcaaa gagacgatcc
     9601 catagcggaa cttcactact gccatttaag gtttgcgaaa atatcaacgc cgccgcaaca
     9661 ggtgtgccaa acagcgcgcc gatggttccg gcagaggcta aaatagtcca ctccattcgg
     9721 ttgacgcgcg gtaacagacg agcgccaatg gcaaccgcaa gggcgatatt gacggtcatg
     9781 atcggatgtt ctggccccag gctgacgcca ccagcaagac caagaattaa tgctacgata
     9841 agtcctggta gcgcagaggg cggaaccggt gcgccgatca gcggttcaca ggcggggtct
     9901 ggtccggcat gaccctggct gaaacggata accaacccca ccgcaatacc cgttagcgtt
     9961 aatacaccga tgatccagag gggtgaatcc tgggctatcc ccagagttcc cggcagtcgt
    10021 tgccagagca aattctgtaa tgccgaggcg attttcatca ccacaatcag aataagactg
    10081 gacgcaatcc caattgccac ggcggggagc gataataaca acatggttct ggctcgcgga
    10141 tggagcatgt tctattcctt atgcggggtc agatacttag tttgcccagc ttgcaaaaag
    10201 gcatcgctgc aattggtgct gaaacgataa agtaaatgtg tgacccagat cgatatttac
    10261 agggagcctg cctttccggc gttgttgtta tgcccccagg tatttacagt gtgagaaaga
    10321 attattttga ctttagcgga gcagttgaag aatgacaaag tatgcattag tcggtgatgt
    10381 gggcggcacc aacgcacgtc ttgctctgtg tgatattgcc agtggtgaaa tctcgcaggc
    10441 taagacctat tcagggcttg attaccccag cctcgaagcg gtcattcgcg tttatcttga
    10501 agaacataag gtcgaggtga aagacggctg tattgccatc gcttgcccaa ttaccggtga
    10561 ctgggtggca atgaccaacc atacctgggc gttctcaatt gccgaaatga aaaagaatct
    10621 cggttttagc catctggaaa ttattaacga ttttaccgct gtatcgatgg cgatcccgat
    10681 gctgaaaaaa gagcatctga ttcagtttgg tggcgcagaa ccggtcgaag gtaaacctat
    10741 tgcggtttac ggtgccggaa cagggcttgg ggttgcgcat ctggtccatg tcgataagcg
    10801 ttgggtaagc ttgccgggcg aaggcggtca cgttgatttt gcgccgaata gtgaagaaga
    10861 ggccattatc ctcgaaatat tgcgtgcgga aattggtcat gtttcggcgg aacgcgtgct
    10921 ttctggtcct gggctggtga atttgtatcg cgcaattgtt aaagccgaca accgcctgcc
    10981 agaaaatctc aagccaaaag atattaccga acgcgcgctg gctgacagct gcaccgattg
    11041 ccgccgtgcg ttgtcgctgt tttgcgtcat tatgggccgt tttggcggca atctggcatt
    11101 aacgctggga acctttggtg gggtttatat tgctggcggt atcgtgccgc gcttccttga
    11161 gttcttcaaa gcctctggtt tccgtgccgc atttgaagat aaaggacgct ttaaagaata
    11221 tgtccatgat attccggtgt atctcatcgt ccataacaat ccgggtcttc tcggttccgg
    11281 tgcacattta cgccagacct taggtcacat tctgtaaatc cttcctgtta tatcgggagg
    11341 taactctccc gataatcttt taaatcatac agttaattca atttttcttt gtgtcccctc
    11401 acaaggccga cctgcgtcac acttccgtac agcgggatta attctccagt aaatgcatta
    11461 tttgtctggt aacggcgatt tgttttgcac gttcataatt tcactcgtca actatcactg
    11521 aatgaggaat taacgatgag taagaaactg attgccttat gtgcctgccc aatgggcctg
    11581 gctcacacct ttatggccgc tcaggcgttg gaagaagcgg cggtagaagc cggttatgaa
    11641 gtgaaaattg aaacccaggg cgcggacggt atccagaatc gcctgacggc gcaggatatc
    11701 gccgaagcaa ccatcatcat ccactccgtg gcagttaccc cggaagataa tgagcgtttc
    11761 gaatcacgcg acgtttatga aatcactttg caggacgcaa ttaaaaacgc tgcgggcatc
    11821 atcaaagaaa tcgaagagat gattgcctct gaacaacagt aatattcgac agggataatt
    11881 gttatggcca ttaaaaaacg cagtgcaacc gttgtgcctg gcgcatccgg tgcggcagcg
    11941 gcagttaaga atccgcaggc ctctaaaagc agcttctggg gtgaactccc gcagcatgtg
    12001 atgtcaggga tttcacgcat ggtgccaacc ttaattatgg gcggtgtgat cctcgctttc
    12061 agccagctga ttgcttatag ctggcttaaa attcctgctg agatcggcat catggatgct
    12121 cttaatagcg ggaaattctc cggtttcgac ctctccttac ttaaatttgc ctggctgtcg
    12181 cagtccttcg gcggcgtgtt gtttggtttt gccattccga tgtttgctgc ttttgtggcg
    12241 aactctatcg gtggcaaact ggcgttcccg gctggtttta tcggcgggtt gatgtctacc
    12301 cagccgacgc aactgctgaa cttcgatccc agcactatgc aatgggcgac ctcttcgccg
    12361 gtgccgtcaa ccttcattgg tgcgctgatt atttctatcg tcgcgggtta ccttgtgaag
    12421 tggatgaacc agaaaatcca gttaccggat ttcctgttag cgttcaaaac cacatttttg
    12481 ctaccgattc tttccgccat ttttgtcatg ctggcgatgt actacgtcat cacccccttt
    12541 ggtggctgga tcaacggcgg tatccgtact gtgctgactg ccgcaggtga gaagggcgcg
    12601 ctgatgtacg caatggggat cgccgcggcg acagcaatcg accttggtgg cccgatcaac
    12661 aaagccgcag gtttcgttgc cttcagcttt accactgacc acgtattacc ggtcaccgct
    12721 cgttctatcg ctatcgttat tcctccgatt ggtctgggtc tggcgaccat tattgaccgt
    12781 cgtttaaccg gcaaacgcct gttcaacgct caactttatc cgcagggtaa gaccgcgatg
    12841 ttccttgcct ttatggggat tagtgagggt gcgattccgt ttgcgctgga aagccccatc
    12901 accgccattc cgtcgtatat ggtcggcgcg attgtcggct caaccgccgc tgtctggctg
    12961 ggcgcagtgc aatggttccc ggaatccgct atctgggcat ggccgctggt cactaacctg
    13021 ggcgtctata tggcggggat cgcgctgggg gcagtcataa ctgcgctgat ggtggtgttc
    13081 ctgcgtctga tgatgttccg taaaggcaaa ttgttaatcg atagcctgta agaaggaaaa
    13141 tccagatgac attactcgct tcgctgcgcg actggcttaa ggcgcaacaa ctggatgcag
    13201 tgcttctctc ctcacggcag aacaaacagc cgcatctggg gatctccacc ggatcaggct
    13261 atgtggttat tagccgtgaa agtgcgcaca ttctggtaga ttcgcgctat ttcgttgagg
    13321 tggaagcccg tgcgcaaggc taccagctgc atttgcttga cgcgacgaac acgcttacca
    13381 ctatcgtcaa tcaaatcatt gccgatgagc agttgcaaac gctcggtttt gaaggccagc
    13441 aggtgagttg ggaaaccgcg caccgctgga agtctgaact caatgcgaaa ctggttagcg
    13501 ccacgccgga tgtgctgcgg caaatcaaaa cgccagagga ggtggagata atccgcctcg
    13561 cctgtgggat tgccgatcgc ggagcggagc atattcgccg ctttattcag gcggggatga
    13621 gcgagcgcga gatagccgct gaactggagt ggtttatgcg ccagcagggc gcagaaaaag
    13681 cctcttttga caccattgtc gccagcggct ggcgcggggc gctgccgcac ggcaaagcca
    13741 gcgacaagat tgttgcagcg ggcgagtttg tcactctcga ttttggtgcg ctgtatcagg
    13801 gctactgctc tgatatgacg cgcaccttgc tggtgaatgg cgaaggggtg agcgccgaat
    13861 ctcacccgct gtttaacgtc taccagattg tcctgcaggc acagctcgca gcaatctctg
    13921 caattcgccc cggcgtgcgc tgccagcagg ttgacgatgc cgcgcgccaa gtgattaccg
    13981 aggcaggttt tagccactat ttcggtcata acaccggtca tgctatcggc attgaagttc
    14041 atgaagatcc gcgtttttca ccgcgggaca ccacgacgct acagccaggc atgttactga
    14101 ccgtggagcc ggggatttat ttgccagggc aagggggcgt gcgcatcgaa gatgttgtgc
    14161 tggttacccc gcaaggcgca gaagtgctct acgccatgcc gaaaacagtg ttgctcacgg
    14221 gagaggcata atggatttat cgctattaaa agcgttgagc gaggcagatg cgatcgcctc
    14281 ctcggaacag gaagtgcggc agatcctgct ggaagaagcg gatcgcctgc aaaaagaagt
    14341 gcgatttgat ggtctgggat cggtgctgat ccgcctcaat gaatcgacag gtccgaaggt
    14401 gatgatctgc gcccatatgg acgaagtggg atttatggtg cgtagcatct cccgcgaagg
    14461 ggcaattgat gtgctgccgg ttggcaacgt gcgcatggct gcccgccagc tgcagccggt
    14521 gcgcatcacc acccgtgaag agtgcaaaat tccaggcctg cttgacggcg accggcaggg
    14581 gaatgacgtc agcgccatgc gcgtggacat tggtgcgcgc tcgtatgacg aagtgatgca
    14641 ggcgggaatt cgtccaggcg atcgcgtcac ctttgataca acttttcagg ttctccctca
    14701 ccagcgggtg atggggaaag cctttgatga tcgcctcggt tgctatctgc tggtgacgtt
    14761 actacgtgag ctgcacgatg ccgaactgcc tgcggaggtg tggctggtgg caagttccag
    14821 cgaagaggtg ggattacgcg gtgggcaaac cgctacccgc gcggtgtcgc cggacgtcgc
    14881 cattgtgctg gataccgcct gctgggcgaa aaactttgat tatggcgcgg ctaaccatcg
    14941 ccagattggt aacggcccga tgctggtgtt aagcgacaag tcactgattg cgccgccaaa
    15001 acttaccgcc tggatcgaaa ccgtggcggc tgaaattggc gtaccgttgc aggcagatat
    15061 gttcagcaac ggcggcacgg acggcggagc ggtgcactta accggtactg gcgtacccac
    15121 agtggtaatg gggcctgcca cccgccacgg acattgcgcg gcatcgattg ccgattgccg
    15181 cgacattttg cagatggagc aacttttatc tgcccttatt caacgtctta cgcgtgagac
    15241 ggttgttcaa ctgacggatt tcagatgatc tcctgattaa cccggagcgg ttatgttaac
    15301 gattcaattt ctctgtcctt tgcctaacgg tctacatgct cgtccggcgt gggaacttaa
    15361 agaacagtgc agccagtggc aaagcgaaat cacctttttc aaccatcgcc agaacgcaaa
    15421 ggccgatgct aaaagctcgc tggcgttgat tggaactggc accttattta atgacagttg
    15481 cagcctgaac attagcggca gcgatgaaga gcaggcgcgg cgcgtgctgg aagagtacat
    15541 cctggtgcgc tttatcgaca gcgacagcgt tcagccgacg caggcagaac tgacggcgca
    15601 tccgctgccg cgttcattaa gccgccttaa cccggattta ctgtacggca atgtgctggc
    15661 aagcggcgtc ggcgtgggta cgctgaccct gttacaaagc gacagcctcg acagttatcg
    15721 ggcaatcccc gccagtgcgc aagattccac ccggctggag cacagcctgg caacgcttgc
    15781 cgagcaactg aaccagcaat tgcgtgagcg tgacggcgaa agcaaaacta tcctcagcgc
    15841 ccatttgtcg ctgattcagg atgatgaatt cgcgggcaat atccgtcgtc tgatgacaga
    15901 acagcatcaa gggctggggg cggcgatcat cagcaatatg gagcaggttt gcgccaaact
    15961 ttctgcttct gccagtgatt atctgcgcga acgtgttagc gacattcgcg atattagcga
    16021 acagttgctg catatcacct ggccggaact gaagccgcgc aacaatctgg tgctggaaaa
    16081 accgactatt ctggtagccg aggatttaac cccaagccag tttttgagcc tcgatttaaa
    16141 aaatcttgca ggcatgattc tggagaaaac tgggcgcacc tcgcataccc tgattctggc
    16201 ccgcgcctcg gcgatcccgg tactgagcgg cctgccgctg gatgcgattg cccgttatgc
    16261 cgggcaaccg gcggtgcttg acgcccagtg cggcgtgctg gcgattaacc cgaatgacgc
    16321 ggtgagcggt tattatcagg tcgcacagac gctggcggat aaacgccaaa aacaacaggc
    16381 gcaggccgcc gcgcagctgg cctattcccg tgataacaag cgtattgata ttgcggcgaa
    16441 tatcggcacc gctctggaag cgccaggcgc gtttgccaac ggcgcggaag gcgttggact
    16501 gttccgtact gaaatgctgt atatggatcg tgacagcgcg ccggacgagc aggagcaatt
    16561 tgaagcctac cagcaggtac tactggcagc gggcgacaag ccgattatct tccgcacaat
    16621 ggacatcggt ggcgataaaa gcattcctta tctgaatatt ccccaggaag agaacccgtt
    16681 cctcggctat cgcgcggtac gtatttaccc ggaatttgct ggcctgttcc gcactcaact
    16741 acgggcgatt ttgcgcgccg ccagttttgg caacgcccag ttgatgatcc cgatggtcca
    16801 cagtctcgat cagatcttat gggtgaaggg cgagatccaa aaagcgatcg ttgagcttaa
    16861 gcgcgatggc ctgcgtcatg cagagacgat tacgctgggg atcatggtgg aagtgccgtc
    16921 ggtgtgctac atcatcgatc acttctgcga tgaggtcgat ttcttcagta tcggctccaa
    16981 cgatatgacc cagtatctgt atgcggtcga tcgtaataac ccgcgcgtat cgccgctgta
    17041 taacccgatt acgccatcgt tcctgcgcat gttgcagcag attgttacca ctgcgcatca
    17101 gcggggcaaa tgggtaggca tttgcggtga actgggcggt gaaagccgtt atctgccgct
    17161 actgcttggg ctgggcctgg atgagctgag tatgagtagc ccgcgtattc cggcggtgaa
    17221 aagccagctt cgtcaactgg atagcgaggc gtgtcgggaa ctggcgcgtc aggcatgtga
    17281 atgccgcagt gcgcaggaaa ttgaagcgtt actcaccgcc tttacgccgg aagaagacgt
    17341 tcgcccactg ctggcgctgg agaatatctt tgttgatcag gattttagca ataaagagca
    17401 ggcgatccag ttcctgtgcg gcaacctcgg cgttaacggg cgcactgaac atccgtttga
    17461 gctggaagaa gatgtctggc agcgggaaga gattgtcact accggcgttg gttttggcgt
    17521 ggcgatcccg cacaccaaat ctcagtggat ccgtcattcc agtatcagca ttgcccggct
    17581 ggtgaaaccg gttgactggc agtcagagat gggcgaagtc gaactggtga tcatgctgac
    17641 gttgggtgct aacgaaggga tgaaccatgt gaaagtcttc tcgcagctgg cgcgtaaact
    17701 ggtgaataaa aacttccgcc agtcattgtt tgccgcgcaa gatgcgcaaa gcatcctgac
    17761 gctgctggaa acggaattaa ccttctgacg ttagccctga aaacgggcgc tgtactctcc
    17821 cggcgtcaga ccaaactgac gccggaaaac gcgacaaaaa tagtcgctgt ccggaaaacc
    17881 gcaacgctgt gccacttcat gaattgacag atgatatttc tgcaaaatca tcctcgcctt
    17941 cgccattcgc acccaacgca catactcgat aaaacgcatc gttccatgct gagcaaacag
    18001 ttttgacaga tgattgggcg tgatattaaa aaactgcgcc acgctctcgc gggtgagcgg
    18061 ctgggcataa ttatcctgaa cccagttaca aatgctgtga tagagaaaat cgccgcgcgg
    18121 ttgcgaatta cccggcggca tattcaccac gttgcggcaa agatgcagca aacttaacac
    18181 cagtggctga atgatattct gctccagcgg cgaacgctaa gatgggtgag ggcggtaagc
    18241 atcgcttcac cttcaccgcg ttgcaggctg ggaagctgga tctggcgagc aggatgcaac
    18301 aacgacgtgg tgcggctatc gtaaaacgat aacccaagcc aggtcggtgc gaacaccagg
    18361 cttaacagca tcaccggttt gttgttgatc ggtaaattag ccgcgcgtgc cggaataaac
    18421 agcatttctc cttccaccat tcgctgctgc tttgcttcca gcaggttgcc gtattcgccg
    18481 cgcagcacga tatccagacg gggaaaatca atacataaac tgcccaccgg taacgaggca
    18541 ggctggctgg caaaccagac gcgccccagt agttgcgggt taagcaccag gccgctgaac
    18601 agatcggcga aaaattgttg atcggcgggc aagcctggtg ctttcatgaa agttcccgat
    18661 taaagatgca ttaactggcg aaattctttc actttgctgc ggctgaccgg cacttcaaaa
    18721 tccagatctt tcagtcgcag aatgtaggtg ttattaaacc acggttcgat ttcgcgtatt
    18781 ttgttcagat tgacacaaaa tgagcgatgg cagcggaaaa aatgcgacgg cggcagtttg
    18841 ctgcaaaatt cggtaatgtt catcggcatt acgtaggatt cacgccgcgt atagacaaag
    18901 gtcattttct cgtgcgcttc ggcgtaatag atatcgttaa ttggcgtgac gattattcgc
    18961 tcatctttca ccagattaat ggtgtcattc tcacgcgtta ccgtcgcggc aggcgtactg
    19021 ctggtctgct gttgttgcca ggccgcttcc agtttttgca gcatcccggt aatacgtgac
    19081 tcctgatacg gtttgagaat gtagtcaaac gcctccagtt caaacgcttc taccgcatgt
    19141 tctttccacg cggtgatgaa cacaataaac ggtttatggg cgaactggct gatgttttgc
    19201 gccagcaaca cgccatccag cgacggaata ttgatatcca gaaaaatggc gtcgacgcgg
    19261 ttatgctgca aaaacttcaa cacgtccaga ccgtcgtcaa aggtgccgac aatctccatc
    19321 tggctgtgct ctttaattag ccagctcagt tcctgttgtg ccaggaattc gtcttcaaca
    19381 atgatgactt tcacaatatc actccggctc aaagcaataa cgtagcctgt gaggcgactg
    19441 gggtgcgttg gttaggaatg taaaacgcaa tttccgtccc cggctccagg cggcggatat
    19501 gcagcccctc gccatacaat aacttcacgc gatgatggac attcagcagg ccaattttat
    19561 tgcccggcat ttcattcgct tcgacccgct caatcacctt tggatcgatg ccgtgcccgg
    19621 tatctcgcac cgcaatgcgt acccgatttc cgcactctgc aacgctgatg gtgacaacgc
    19681 ctttaccttt gcaaggctga ataccgtgga caatggcgtt ctccaccaac ggctggatca
    19741 gcaggctggg aatgcagcaa ttcacctctt catcaatatc atagataacc gtcagcttgt
    19801 caccaaagcg ggcctgctca atggcaatat agtctttaat ttgatacagc tcttttttga
    19861 tatcgatttg ctcatcgtct tttaattcaa tgttatagcg cagataacgc gacagattaa
    19921 agatcaactg gcgagcggta tccggattca gacggattga cgatgaaata gcgttcagag
    19981 cgttaaacag aaaatgggga ttaattttgc tttgcagggc gcgaagctct gccttatttg
    20041 ccatttcacg tagctgctcg gcgcgtgaaa cctccagttg cgtggagatg atttgcgaca
    20101 gaccgaccgc catttcctgt aatgacgagg tgatctgatg cgcgtggcag tagtaaattt
    20161 tcagcgttcc ggtcacgacc cctttctccc acaatgggat caccagcatg gaatgaatct
    20221 ctggtgtgcg gtgggcttca tcattgtttt taatgatgat ttttccgtaa ttcatcgcct
    20281 gacgggtagt cgggctaatg aagtcatcgc cattctgata gttatgttca cccacgccaa
    20341 cataggccag cacatgatcg gtattggtaa ttgccaccgc atcggcgtga atgtcatcgc
    20401 ggataatttc gcagaccttg cgtaatgact cgctattgac atggcgaaac agcggtagcg
    20461 ttttgttggc gatatccagc gccagcttgg cctgccgcgc ggcgctggcc tctttttcgc
    20521 cctcaacgct ttgcaccaga agcacaataa agccgataca gacgctaccg aggatcattg
    20581 gaatgccgat tttagagacg atatcgatcc ccaacgcggt agttggtgcc cagacaatta
    20641 ccagaatcat ggtcaacgtc tcacacaaca tgccgccgag aataccgacg cgccagcgct
    20701 gtgctttggg gattttcagg ttgatccagc ccgatataca acccgccaga atgctggtga
    20761 taaagcaggg gatcgccgtc acgccgccga tatcaattaa ataccggtga atacccgcaa
    20821 tcacgccggt aatgatgcct acccacgggc caaacagaat cccgccggac atcaccgcga
    20881 taatacgtac gttcaccagc gagccttcta cgggaacgcc ggaccaggtg ctgaacaggg
    20941 cgaacagcga gaaaatggcg gtaacggcga gcaattcttt tggggagtgc gccgacttgt
    21001 gcaacagttc gcgaaacagg cggatacgga tgagaaagaa caggcagata agcattaacg
    21061 ctgcccgatc gaagaccgcc agcagcatgt tgaatatttc gtgcacgggt gactctgaat
    21121 aaggattaaa cctctatgat aggtaacctg aaggctgatg accagcaggc cgtttttgag
    21181 gaaaaaccat aaatttaaaa ggggattgaa agataaatgg tgtattagca atgtatctct
    21241 tgataatgca cttctggctg gtaaatttat tgaaattata atgttaataa aattggtggt
    21301 aattttcttg ttagcgtaga aaaatttcct gtttttgaag tggagttcct catttaaagc
    21361 cattgataaa aatgttctaa aacgctggac agacagaaat taatcaggct attttcaaaa
    21421 cagccaccat tgtggcagcg tcgctcggac ggtccgggcg ctaacgttaa tctgaggaaa
    21481 ttatggctga cactcgccct gaacgtcgct ttacgcgcat tgatcgtctc ccgccctacg
    21541 tttttaacat caccgctgaa ctgaaaatgg ctgcgcgtcg gcgcggcgaa gatattatcg
    21601 atttcagcat gggtaacccg gacggtgcga ctccgccgca tatcgtcgaa aaattatgta
    21661 ctgtggccca gcgcccggac acgcatggtt actccacttc acgcggcatt ccgcggttac
    21721 gtcgcgccat ttcccgctgg tatcaggatc gctacgacgt tgaaatcgac ccggaatcag
    21781 aagccatcgt cactattggt tcgaaagagg gcctggcgca tctgatgctg gcgacgctgg
    21841 atcatggtga cacggtgctg gtgccgaatc caagttaccc gatccatatt tatggcgcgg
    21901 tgattgccgg ggcgcaggta cgctcagtgc cgctggtgga aggtgtcgat ttcttcaacg
    21961 aactggagcg cgccattcgt gaaagttatc cgaaaccgaa gatgatgatc ctcggcttcc
    22021 cgtcgaatcc aaccgcgcaa tgcgtggagc tggagttctt cgaaaaggtt gtggcgctgg
    22081 cgaaacgcta cgatgtgctg gtggtccacg acctggccta tgccgatatc gtctacgatg
    22141 gctggaaagc gccgtcaatc atgcaggtac cgggtgcacg cgatgtggca gtcgagttct
    22201 ttacgctgtc gaaaagctac aacatggcgg gctggcgtat cggctttatg gttggcaaca
    22261 aaacgctggt cagcgccctg gctcgtatta aaagctatca cgattacggc acctttacgc
    22321 cgttgcaggt ggcggcgatt gcggcgctgg agggcgatca acagtgcgtg cgcgacattg
    22381 ccgaacagta caaacgccgc cgtgatgtat tggttaaagg gctgcatgaa gcgggctgga
    22441 tggttgaaat gccgaaggct tcgatgtatg tctgggcgaa aatcccggaa ccatatgcgg
    22501 ccatgggatc gctggaattt gccaagaagc tccttaacga agcgaaggtt tgtgtctcgc
    22561 cggggattgg ctttggcgac tatggtgata cgcatgttcg ctttgcactg attgaaaacc
    22621 gcgaccgtat tcgtcaggcg attcgtggga ttaaagcgat gttccgtgcc gacggtttac
    22681 tacccgccag cagcaaacat attcacgaaa acgcggaata aacaccgtcg tcagaaatgc
    22741 aaacaggagc ctgatggctc ctgtttttca ttgcacagtt tgcttttaga tcatcagggc
    22801 aaaagtaccg gcccaaacga tgaccataaa tgaaatgccc ataaagaaat atttcacttt
    22861 acatctctcc gcgtaattac tgttacgcct gaatgacttc attacacctg atacagtgag
    22921 ttgacaccgg gcaaagcgac cttaaaagcc ctgtatttta ccgtgggcat caccccagaa
    22981 ttctgagcta aactgctcga gacggcgcta atgtacgcct aaaactgaaa cgagacaaga
    23041 gacattttct tatttacttt tagtaactta caagtgtctc atatcggcga cagtttaatt
    23101 tctgacggta ataattagat attcagcgaa ggaaaaaacc ttaagaaaaa agccataaaa
    23161 accatgaggt tattatggcc gatttgagga gggaaagagt aagagcagtt tgttaaatgt
    23221 acaacgacga ttctcccacc gggcgcgttt taaagcgacg gtggatccag aggtactgct
    23281 ccggtgcgcg catgatctct ttctcgataa tcttgttcat ataggcagcg gcttgatttt
    23341 catctgtcgg gtagccttcc atctctgggg tgatgaacaa acgatatccg ctgtaatccg
    23401 cttttcttac catcgttacg gtcaacatgg ctgcgccaga gagacgggag agaacatagg
    23461 tgccattggt tgtggcgaca ttttccaccg caaagaacgg cgcgaaggag ctgcctttac
    23521 gaccataatc ctgatcggga gcaaaccata ccgcttcacc tttcttcagt gcaccgacaa
    23581 tgccgcgcag attatttctg ccgatcatcg ctttgttaga gcgcatgcgc ccacgggtct
    23641 gcacccattc catcagctga ttattatgtg gacgataggt agccatcatt ggttggcaca
    23701 gtcccatcac gcggccgccc agttccagcg acataaaatg gacgccgaca accattacgc
    23761 cgcgattttg catttgtgcg cgtttaaggt tatccaaccc ttcaacatca aaccatttac
    23821 gtacgcgact gtcgggccag aaccatgcca tgccggtttc taccagcgcc atgccgagtg
    23881 aacgaaagtt ttcggcaatc atcttctcgc gttcttccgc agaatgctgc gggaaacaaa
    23941 gttccaggtt tttacgggcg atagattcac gacgtttcag gaatggtcgc gccattgcgc
    24001 caatacgcgt gccgagaaag cagagaacag gataaggaag ctgtacccaa agccagagta
    24061 caccaagccc aaaccatgtg agccagtagc gaggatgtag aaactcgcgg gaaaatttgc
    24121 attgtggaaa catagttacc ttctgtagat acgaattgtt gtatcgcaga agattccatg
    24181 tcgacagcgg tacactcaat tggttgggcc atcatgtacg acaatggttc ctgtaaagag
    24241 tgtaaagact agtcaggaca taaccacgag gagcatgaat gcggtatctg tactttatca
    24301 ctcgctgact gctttcagct actgattatt aatcgtgcaa agtaaatatt tttccgtaca
    24361 caacagtctt gcatttaaca tgctgttttc attgatgctt agcaaaaatt gccagaggtt
    24421 aaggattgtc ttactattaa agtgactgaa gttacaggtt ttttagcgcg gcaggaggtt
    24481 gagctatgat ttatttatgg atgtttctcg ccctgtgtat tgtctgcgta agcggctaca
    24541 tcggtcaggt actgaacgtg gtatcggcgg tctcttcttt cttcggcatg gtgatcctcg
    24601 ctgcactcat ttattacttc accatgtggt taactggcgg taatgaacta gtgacgggga
    24661 tatttatgtt tcttgccccg gcttgtggct tgatgattcg ctttatggtg gggtatggca
    24721 ggcgatagtg ggtcaggatg gctcatttgg gcaggtgttc acatcagcat cagacatgat
    24781 tgttcacctg ccatgtgaca gacgctatcg cacaggatga ataagataat tcgcctttgt
    24841 cggataagta ggcgtgattt cttctgtgag gtttaatggt ggcgcatctt ctctcatctt
    24901 aaacaaaatc gcctgtgatg gctgaagttt tgtagagtgt gtggtcgtca tcgctgatgt
    24961 tttttcagtc tctttatagc tcctctcact tacaccacca gaaatggaaa atgcaaaaac
    25021 attgcatgcc ataatagaga ccaataaaaa taagctgaaa agtattaagt ttactttcat
    25081 agaaagttat cctgaaaatg gttcactgag aacctacg
//
LOCUS       DAPHJE010000065        24619 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPHJE010000065 DAPHJE010000000
VERSION     DAPHJE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.065"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            173..889
                     /gene="nanC"
                     /locus_tag="RH222_002672"
     CDS             173..889
                     /gene="nanC"
                     /locus_tag="RH222_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV3797669.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            909..2015
                     /gene="nanM"
                     /locus_tag="RH222_002673"
     CDS             909..2015
                     /gene="nanM"
                     /locus_tag="RH222_002673"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV3797670.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLITVKDNKVTVQN"
     gene            2080..3060
                     /locus_tag="RH222_002674"
     CDS             2080..3060
                     /locus_tag="RH222_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HDV3797671.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGACWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            complement(3643..4883)
                     /locus_tag="RH222_002675"
                     /pseudo
     CDS             complement(3643..4883)
                     /locus_tag="RH222_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            4999..5244
                     /locus_tag="RH222_002676"
                     /pseudo
     CDS             4999..5244
                     /locus_tag="RH222_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            5621..5878
                     /locus_tag="RH222_002677"
     CDS             5621..5878
                     /locus_tag="RH222_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797672.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            5890..6435
                     /locus_tag="RH222_002678"
     CDS             5890..6435
                     /locus_tag="RH222_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3797673.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCADPDETGALT"
     gene            6491..7237
                     /locus_tag="RH222_002679"
     CDS             6491..7237
                     /locus_tag="RH222_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV3797674.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQLPATEEIAQACGVSS
                     TSDFLTLPGLVGAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNIAPKRYVTYARECFGWGVFALIAR"
     gene            7406..7624
                     /locus_tag="RH222_002680"
                     /pseudo
     CDS             7406..7624
                     /locus_tag="RH222_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7662..7778
                     /locus_tag="RH222_002681"
                     /pseudo
     CDS             7662..7778
                     /locus_tag="RH222_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            8023..9144
                     /locus_tag="RH222_002682"
     CDS             8023..9144
                     /locus_tag="RH222_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDV3797675.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTMELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            9141..9419
                     /locus_tag="RH222_002683"
     CDS             9141..9419
                     /locus_tag="RH222_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3797676.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTRINDDALKQQIKALLTQ"
     gene            9431..10744
                     /locus_tag="RH222_002684"
     CDS             9431..10744
                     /locus_tag="RH222_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV3797677.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLLPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            10757..11563
                     /gene="sgcQ"
                     /locus_tag="RH222_002685"
     CDS             10757..11563
                     /gene="sgcQ"
                     /locus_tag="RH222_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HDV3797678.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            11694..12125
                     /locus_tag="RH222_002686"
     CDS             11694..12125
                     /locus_tag="RH222_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3797679.1"
                     /translation="MINDIKWVQAQRKATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLTAVGNASTDDALFALVSG"
     gene            12137..12769
                     /locus_tag="RH222_002687"
     CDS             12137..12769
                     /locus_tag="RH222_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HDV3797680.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            12786..13568
                     /locus_tag="RH222_002688"
     CDS             12786..13568
                     /locus_tag="RH222_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3797681.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IVNQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            13871..14659
                     /locus_tag="RH222_002689"
     CDS             13871..14659
                     /locus_tag="RH222_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDV3797682.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVDCGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            14664..15569
                     /locus_tag="RH222_002690"
     CDS             14664..15569
                     /locus_tag="RH222_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDV3797683.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMTLAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSITLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            15580..17547
                     /gene="yjhG"
                     /locus_tag="RH222_002691"
     CDS             15580..17547
                     /gene="yjhG"
                     /locus_tag="RH222_002691"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HDV3797684.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDL
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTRLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            17654..19003
                     /locus_tag="RH222_002692"
     CDS             17654..19003
                     /locus_tag="RH222_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDV3797685.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            19350..20336
                     /locus_tag="RH222_002693"
     CDS             19350..20336
                     /locus_tag="RH222_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDV3797686.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(20377..21243)
                     /locus_tag="RH222_002694"
     CDS             complement(20377..21243)
                     /locus_tag="RH222_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HDV3797687.1"
                     /translation="MGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGI
                     IFNISNILLVASINLAGMAVAFPVGVGLALALGVITTYIGNPQGDLLILFLGVVCVVI
                     AIIFTAIAYGRVTQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLM
                     TPYSALVLFAVGLFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWC
                     VGLAFSLIASGQAGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISY
                     IVGIVLIIAANQ"
     gene            complement(21484..22125)
                     /locus_tag="RH222_002695"
     CDS             complement(21484..22125)
                     /locus_tag="RH222_002695"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV3797688.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            complement(22156..23271)
                     /gene="mtnA"
                     /locus_tag="RH222_002696"
     CDS             complement(22156..23271)
                     /gene="mtnA"
                     /locus_tag="RH222_002696"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HDV3797689.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMAA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(23281..24540)
                     /locus_tag="RH222_002697"
     CDS             complement(23281..24540)
                     /locus_tag="RH222_002697"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HDV3797690.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
BASE COUNT         6322 a         6049 c         6025 g         6223 t
ORIGIN      
        1 aataatgtga aaagagattt ttcacaggag accttataca aaaaaatata aaatacagct
       61 accggttgcc aaagacacta taagcctggc aaaaaaatat tacacaacat aaatgctaat
      121 tgtttatgcg ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa
      181 ggctaaaata ctttctggcg tattattact gtgcttttcg tccccattaa tttctcaggc
      241 tgcgacactg gacgtacgtg gtggatatcg tagtggaagc cacgcctatg agactcgact
      301 caaagtcagt gagggatggc aaaatggatg gtgggcaagc atggaaagta atacctggaa
      361 taccattcat gataataaaa aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa
      421 ttacgcgatt aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa cgcattttag
      481 cagcaacggc acacgctacg gaccctacgt aaaactgtcc tgggacgcga caaaagatct
      541 taattttggc attcgctatc gttacgactg gaaagcttac cgacaacaag acttatccgg
      601 tgatatgtct cgtgataacg ttcatcgttg ggatggatat gtcacttacc atattaatag
      661 tgatttcacc ttcgcatggc aaacgacgct atacagcaaa cagaacgatt atcgctatgc
      721 aaaccataag aaatgggcga cggaaaatgc atttgttcta caataccata tgacgcccga
      781 tattacgcca tacatagaat atgactacct tgaccgtcag ggtgtttaca acggcagaga
      841 taatttatcg gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt agacaggaga
      901 cagtcacaat gaataaaaca ataacggcgc ttgctatcat gatggcttca tttgccgcaa
      961 acgcgtctgt attaccggaa actcctgtgc catttaaaag tggtaccgga gcaattgata
     1021 acgacactgt ctacattggt ttaggtagcg caggtacggc atggtacaag ctggatacac
     1081 aggccaaaga taaaaaatgg acagcgttag ctgcattccc tggcggacca agagatcaag
     1141 caacctctgc atttattgat ggcaatctgt atgtgtttgg cggcattggc aaaaacagcg
     1201 agggcttgac tcaggtattt aatgacgtac acaaatacaa ccccaaaacc aatagttggg
     1261 ttaaattgat gtcgcacgcg ccgatgggca tggcgggcca tgtgactttt gtacacaacg
     1321 gcaaggctta tgttactggt ggtgttaacc agaatatctt caatggctat tttgaagatc
     1381 tcaacgaggc tggaaaagat tcaaccgcta tagataaaat caatgctcac tattttgaca
     1441 aaaaagcaga agattatttc ttcaataagt ttctgttgtc ttttgatccc tcaacacagc
     1501 aatggagtta cgctggcgaa tcgccctggt acggaacggc tggtgcggcg gttgtgaata
     1561 aaggtgataa aacctggctt attaatggcg aagccaaacc aggattgcga acggatgccg
     1621 tatttgaact tgatttcacc ggtaataatt taaaatggaa taagcttgct cccgtctcat
     1681 caccagatgg cgtagctggc ggttttgcag ggataagcaa tgattctctt atatttgccg
     1741 gaggggccgg attcaaaggt tcacgagaaa attaccagaa cggtaagaac tatgcgcatg
     1801 aaggcctgaa aaaatcatat agcactgata ttcatctttg gcataacggg aaatgggata
     1861 aatcgggtga attatcgcaa ggtcgggcct acggagtatc attgccctgg aataatagtc
     1921 tattgattat tggcggtgaa actgcaggcg gcaaagcggt gacggattca gttttgatca
     1981 ctgtgaagga taataaagtc acagtacaaa actaacgctt cagggccccg gtaaaggggc
     2041 cctgctatca atttgtcatt tcaattacgc gaaatttata tgaacgcaat aatatcgccc
     2101 gattattact atgttcttac cgttgctggt cagtctaatg ccatggcgta tggcgaagga
     2161 ctgccattac cggacaggga agatgcgcct catcccagaa ttaaacaatt agcgagattt
     2221 gcgcatacgc atcccggagg cccgccatgt cactttaacg acattattcc actgactcac
     2281 tgcccacacg atgttcagga tatgcagggt tatcaccatc ctctggcaac gaatcatcaa
     2341 acacagtacg gcaccgttgg ccaggcactg catattgcac ggaaattact gcccttcatt
     2401 cctgataatg cagggattct tatcgttccg tgttgccgtg gcggatcggc ttttaccgcg
     2461 ggcagcgaag ggacatattc agaacggcac ggagccagcc atgatgcttg tcgttgggga
     2521 acggatactc cgctgtacca ggatttagtc agcagaacgc gagccgcact ggcaaaaaat
     2581 ccgcagaaca aattcctcgg cgcatgctgg atgcaaggcg aatttgactt aatgaccagt
     2641 gactacgcgt cacaccctca acactttaat catatggttg aagcctttcg tagggatcta
     2701 aaacaatacc attctcagct taataatatt actgacgcgc cgtggttttg cggcgatacc
     2761 acctggtact ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg caattatcaa
     2821 aataatgttt tagccaatat tattttcgtc gacttccagc aacaaggtga aagaggactg
     2881 acgaacgcgc ctgatgaaga cccggacgat ttaagcacgg gatattacgg ttcagcgtac
     2941 cggtcaccgg agaactggac gacggcactg cgaagcagtc atttcagcac tgcagcccgt
     3001 cgggggatta tttctgacag atttgtagaa gcaattttgc agttttggcg cgaaaggtga
     3061 gcggggagtt tattcatggg ggatgtcctt gttcgttctc agagtagaca acaaaaaaca
     3121 aatgagatca aggtaatatc tcttcacttg cgggctcgaa cagcaggcaa ttgccaatat
     3181 aataatctgg cacaagggtg aaaaacgcgc accgcataaa ccgttgttat tgcttacaga
     3241 ttagccggat gccttaatag acatccgcgc cttttcgatt atggctcgga aatctacgaa
     3301 catctacaca gtttactgga acgttttggg ccacagcgtt cgcagcaccg tcctgatatg
     3361 ccgttctggc gattataagt cgacggattc tggcaaatag acgatgatga tatgcttaat
     3421 gatgttcaaa gaaattaggc cattattata ttattgcttg cattttcttt cattcctaaa
     3481 agcacccgcc attaatccca ggcgcattat gcaaaggaaa atgattaggt aaacaataag
     3541 atttataatc aggaaaggcg actcgcaata aaacggaaag tcgccttaac attggtaaaa
     3601 tatgcccttt actactcctg tatcatgaac ggctcatcac atttaaagct gacgttgatc
     3661 cagactacca tatattgcct ttatttcatt ttttacgcct tcaccacggt acaccaaaga
     3721 cgtatcgtat cgttgatact tatcttcccg tgtagcactg aaccagttaa aagatccgac
     3781 acacagcagt tcttcatcct caatcacaat tttgctgtga acacgattaa ccaatttcgt
     3841 agcgataccc atcttattga gcttttcaac agcatcattc agcagatgtt gtttctcctg
     3901 acgcttatca tcatcgacat gcgctatatt gcagttttta tccgtcacca cggtaatatc
     3961 aatgccccgg gaacgggcca aagccattga tgccagaaat cccgtttgct ccactttttg
     4021 ccaggataac caaggggaaa taatcgttat tttcttttga gctccggcaa gagtcttgtt
     4081 caaaaattca tcatgctgct ccacaccatg cagcgttgaa atttgtgtat gtgcggaaat
     4141 taaatcctgt cgtttctgaa actcaaattg caacgcatta ttatctgaag aaaagagata
     4201 ttttgccaat aaccctcgcg gcgaaaatgc gggctgcatc tcgataaggt ccatatcgcc
     4261 aaataccaga aaactatctt tagcgcgtga gacagcaaca ttgaggatag tgctgttgct
     4321 atcaagaaac ctgccatctt catgttttga gtagacagga gaaaagagaa caatcgccct
     4381 ttccgccccc tgcaaggagt gcacagtccc cactgtcagt aaaccctgct cgtctttgcc
     4441 attaatttcc agcttacgca atgacatttt gatagcatta acctgcgccg aaaatggcgt
     4501 cacaacacca acaactttgt acagtggttc cccgtaatgg cgctctatat catccttatg
     4561 tgccaccagc catgctgcta ttgtttcagc ttcaagaggg ttatgacggc tccctccatt
     4621 tggcttcatg cctctaccat caatatgtaa atatcccatt gcggggaaaa tcgtcctttt
     4681 tccatccctc ttttaggctg caacttaccg tgatagcaaa gtgagttaca gtagtcgatg
     4741 atgttattga aacagcgacg atgctcatac agatacattc cgcgctcgaa cttgggatca
     4801 tactggtagt gagagttgaa ctgggctacc tgcattacgc tgccagatgc tgcacttttt
     4861 ccagacccat ttattatcga atacggtgac cgcctgagcg gccaccttta atcgtgggca
     4921 gttacacaga attatttaca gggtcttgct gggagttctt acacttgctc gcgatattca
     4981 attggccatt gtcaacaaat gacgcaggtt ttgattggtg atatctattt acgtcagcaa
     5041 tgcgatgtgt tttggctcgg ctataccatc agtcccgctt atgctcgcca gggatatgca
     5101 attgaagcaa ttaccgccac gattgactga ataaaagaga acggtttttc actcatcaaa
     5161 gccggtgtga atccggaaaa taccctttca aaaaaattac ttatcaggat tggcttcaat
     5221 ttttcaagca tcgaagatgg gtgagcatat ttatgttctg gatttaacaa aaggagaata
     5281 atctcccgaa gggaaaagcc gatgaggcca tttcaggcgt acttcaacat gcattcgtca
     5341 gtgtcatctg ccttttccag taaatgggtt ggcattcaat ttccaaagta catcacactt
     5401 tttctactga aacatatccc tcctataact acgtgttaat attcgctccg taaatacctt
     5461 tcaactcaac ccgaggcttt gatgctggag aatgtaatca tccgataatc agttccccga
     5521 ccttttcagg ccggactgat tatcaatgcg ccgaaatcga atgcggacac cgcggtgtgt
     5581 ttgcacgttt tgcacatgat gattaaatag gttcttcagt atgaatttat cccgtcagga
     5641 acaacatacc ttacacgttc tcgctaaagg tagacgtatt gcgcacgtcc gcgattcttc
     5701 aggccgcgtc acttccgttg aatgctacag ccgcgaaggg ctgttgctga ccgactgcac
     5761 gctcgccgtc ttcaaaaaac tcaaaaccaa aaaacttatc aagtccgtca atggccagcc
     5821 ctatcgaatc aacaccaccg agctgaataa agttcgcgct cagctcgata atcgctaagg
     5881 agagtttcta tgacagtaca ccacttcact tttcatatca cagataaaag tgatgccagt
     5941 gatatccggg aagtcgaaac ccgcgcattt ggcttcagca aagaagccga tttagtggcc
     6001 tctttacttg aggatgaaag cgcgcgcccc gcgttatcgc tgctagcccg gtatgaaggc
     6061 aaagccgtcg ggcatatttt atttacccgg gcaaccttca aaggagaaat ggattcaccg
     6121 ttgatgcaca tcctcgctcc cctggcggtc ataccggaat accaagggat gggcgtggga
     6181 ggacggttga tacgcactgg catcgagcac ctgaggttga tgggctgcca gacggttttt
     6241 gttctcggcc acgccaccta ttacccccga catgggttcg aaccctgtgc cggagacaaa
     6301 ggctatccgg caccttatcc catcccggag gagcacaaag cctgctggat gatgcaatca
     6361 ctcaccgctc agcccatgac cctgaccggc cacatccggt gcgcagaccc tgatgaaacc
     6421 ggagcactga cgtgaataac aatcttgttg tgtccttttt aaggcttaac ccaataacag
     6481 gaattaaata atggatatcc cacgtatttt taccatcagc gaaagtgaac accgcatcca
     6541 taacccgttc accgaagaga agtacgccac gctgggtcgc gtgctgcgca tgaagccggg
     6601 cacccgcatt cttgacctcg gcagcggctc gggcgagatg ctctgcacct gggccaggga
     6661 tcatggcatt acggggactg gcatcgacat gagttcgctc ttcaccgcgc aggcaaagcg
     6721 tcgcgcggaa gaactcggcg tcagcgaacg cgtacatttt attcataacg atgcggccgg
     6781 ttacgtcgca aatgaaaaat gtgacgtggc ggcctgcgtc ggcgcgacat ggattgccgg
     6841 tgggtttgcc ggggcggagg agctgctagc gcaaagcctg aaaccgggcg ggatcatgct
     6901 tatcggcgaa ccctactggc gtcagctacc agcaacagaa gaaatagccc aggcctgcgg
     6961 cgtcagctcg acaagcgatt tcctgacgct gccaggactt gtcggagcgt tcgacgatct
     7021 cggctatgac gtggtggaaa tggtactggc agaccaggaa ggctgggacc ggtatgaagc
     7081 cgcaaaatgg ctgaccatgc gccgctggct ggaagcgaat cctgacgatg acttcgccgc
     7141 cgaagtcagg gccgaactaa acatcgcgcc gaaacgctac gtgacctacg cgcgggaatg
     7201 ctttggctgg ggcgtgttcg cactcatcgc ccggtaacaa caggttcccg gtatttctgg
     7261 ctggaaatat cgaggcttta cctttgcttt gatctgatgt atcacctgca caatacatat
     7321 tcagtatgtt tattaatcaa gatgtcacca taaggagcat catatgtcac aacatgaaca
     7381 gttctgtcag gcttgcggaa tgccgatgtc ggcaccagat gcacacagtg ccagtgatca
     7441 atattgtgcg taatgcagcg acagcaacgg caatctcaaa ccctgggaag aagcggtatc
     7501 aggtttagcc agcttccttg attcctggca aaaagtcggc ccggaggaag cacataagcg
     7561 tgcaaaacgt tatctgacgg caatgccagc ctgggctcat aaagcggaga gtgatttgag
     7621 ctgattatct atactccctc tgaatctatt caggagatcc gatgttttct tacattatgc
     7681 tgggaaccaa cgacctgcca cgcgccatta cattctaaga tccgctgatg gagatgctgg
     7741 ggtatccacg tgcaggacgt aatgaagagg gagcgtcctg caacaaagtg gtgctataac
     7801 agttaccgcg ttttattcat cctcattatt tcaaccataa tcactggtct ctccagcccg
     7861 taactttcac aaacgttgag cgcttttatt ccactcggct cacataattt tttatttgtt
     7921 cagctttgcg cgttttcacg tgaacgttct cacaattatt cgaccagtct gcgcctataa
     7981 aacgctcaac gagtgattgt tttacggtct acggagatag ctatgtcatt ttctgtgcag
     8041 gaaaccctgt tctcgctttt gcagcacaat gcgatttcag gacacgaaaa cgctgtcgct
     8101 gacgtcatgc tgtgcgaatt caggcgtcag gcaaaagagg tctggcgaga caggcttggg
     8161 aatgtcgtcg cgcgctacgg tagtgataaa cccgatgcgc tgcgactgat gatttttgcg
     8221 catatggatg aagtcggttt tatggtgcgc aaaatagagc cgtctggatt tttacgcttt
     8281 gaacgcgtag gcggtcctgc gcaggtcact atggctggtt ccatcgtcac cctcaccggg
     8341 gacaaagggc cagtcatggg gtgtatcggc attaagtcct accactttgc caaaggcgac
     8401 gagcgcacgc agtcaccttc tgtcgacaaa ctgtggattg atattggtgc caaagacaaa
     8461 gacgacgcta tacggatggg cattcaggtc ggtacgcctg tcactctgta taacccgccg
     8521 caactcctgg caaacgatct ggtgtgcagt aaagcactag acgatcgtct aggctgtact
     8581 gccctgctcg gtgtagcgga tgctatcagt actatggagc ttgatatcgc cgtttatctg
     8641 gtggcttcgg tacaggaaga atttaatatc cgcggcattg ttcccgtatt acgccgtgta
     8701 aaacctgacc tggcgattgg tattgatatc actccatcgt gtgacacccc tgatttacac
     8761 gattattccg aggtcaggat taatcagggc gttgggatca cctgcctgaa ctaccatggt
     8821 cggggaacgc tggccggatt aatcacgcct cctcgcctga tacggatgtt ggaacagacg
     8881 gctcttgaac acaacattcc ggtgcagcga gaagtggctc ccggcgtgat aacagaaacc
     8941 ggttatatcc aggttgagca ggatggtatt ccctgcgcca gtctctctat tccttgtcgc
     9001 tatacccatt ctccggcgga ggttgccagc ctgcgtgatt tgactgattg catccgccta
     9061 ttgaccgctc tggcaggtat gtcagcagca catttccccg ttgagcctga ttcaggcact
     9121 acacaagagg cacatccatt atgaaaaaga tccttgtggc atgcggtacc ggcatgtcga
     9181 cttcaaccat gattgcacac aagctgcaag agttcctgac tgaacagggc atttcagcca
     9241 ctaccgccca atgctgtctg aatgagatcc ctcttaactg taacggtatg gaccttattg
     9301 taacctccat gcgaaccaac agtgattacg gcatccctac gcttaatggc gccgccctgc
     9361 tcacaaggat caacgacgac gcgttaaaac aacaaatcaa ggctctgtta actcaataaa
     9421 aggaggcatt atgtttgatt acatcctgtc tctcggcggt accgttttcg tccctatcat
     9481 catgatcgtg attggcctta tcttccgcat cccctggctg caggcgatta aggcaggggt
     9541 aacggttggg attggttttg tcggtatggg actggtgatc gtcatggcga tcgacagcct
     9601 cagtccccct atcaaagtga tgattgagcg tttcggccta gccctgcatg tctttgacgt
     9661 tggcgcaggc cctgcctccg gcgttggtta tgcgacagcc attggcgcca tgattatccc
     9721 agttattttt cttctcaatg tcgccatgct ggtcacccgt ctgaccaaaa cgatgaacgt
     9781 cgatatttac aattactggc actacgccat tacgggtacg gtggtccagc ttatgaccgg
     9841 tagcctgatt tatggtgtgc tgggggcaat ctgccatgcg gcgctatcgt taaaaatggc
     9901 cgactggacg gcaaaacggg tacagaacat cgtcgggctg gaaggtattt cgataccgca
     9961 aggctacggt tccagttcag taccgctttt cgttctactt gatgctatct atgaaaaaat
    10021 ccctttcatg aagggacgca atatcgatgc tcaggagatc cagaaacgct acggcatggt
    10081 cggcgatccg gtgattatcg gcgtagtgct cggtctgatt tttggcctgg cggctggtga
    10141 aggctttaaa ggttgcgcca gtctgatgat taccgttgcg gcaatcatgg tgttgttccc
    10201 gcgcatgatc cgcctgattg ttgagggcct gctgcccatc tccgacggtg caagaaagtt
    10261 cttccagaaa tatttcaaag gacgtgaagt gtatatcggt ctggatactg ctgtgacgct
    10321 ggggcatcca accaccattg ctgtcggtct gctactgata cccattatgt taattctggc
    10381 cagcatttta ccgggcaaca aagtgttacc cctcgcagac ctgccggtag caccgttctt
    10441 tatctgtatg gctacggtta tccaccgcgg agacctggtc aggacgttga taagcggggt
    10501 catcgtgatg attaccgtcc tgctgatcgc cacccagttc gcgccttact tcactgaaat
    10561 ggcgctcaaa ggcggtttca gttttgccgg tgaaagtgcg caaatctcag cgttgtccgt
    10621 gggcaatatg ttcggttggt cgatttctga attgatgtcg cttgggatta ttggcgttgt
    10681 cgtggcggtc ggtattgtcg ccagcgtcgt gctgttctta cgtaaacgtg aattatcgga
    10741 ataaggagta ctttcaatga gttggctgaa agaggttatt gggacggaaa aagcggtcat
    10801 cgccatgtgc catctacgtg cattgcccgg cgacccgagt tttgatgcgc agttggggat
    10861 gaactgggtt atcgacaaag cctgggacga tctgatggcg ctccaaaatg gtggggtgga
    10921 tgcagtcatg ttctccaacg agtttagtct tccctatctc acgaaggtgc gtccggaaac
    10981 cactgcggca atggcacgaa tcatcgggca attaatgagc gatatccgca tcccgtttgg
    11041 cgtgaatgtt ctgtgggatc cggtcgcctc attcgacctg gcgatggcta ccggcgctaa
    11101 gtttatccgc gagatattta ccggtgccta tgccagcgac tttggcgtct gggacactaa
    11161 cgtcggtgag accatccgtc atcaacaccg gattggtgca ggcgaagtga aaaccttgtt
    11221 caatatcgtc ccagaggccg ccgtgtatct gggcaatcgc gatatctgtt ctattgctaa
    11281 atcgacagta tttaacaacc atcctgatgc gctctgtgtt tctggcctga ccgccgggac
    11341 gcgtaccgac agcgcgctgt taaaacgggt caaagaaacg gtgcctgaca ccgtcgtgtt
    11401 ggccaacacg ggcgtctgcc tggaaaacgt ggaggaacaa ctcagtatcg ccgatggctg
    11461 tgtgaccgca accaccttta aaaaggacgg cgtttttgcc aatttcgttg accaggcgcg
    11521 agtcagtcag tttatggaga aagtgcatca tatacgccga taagatgggg ataagcagag
    11581 cgcttatccc cggcaaagag caataaccct tctgcgatcc ctgccgcctt cgtcaaaagt
    11641 atcgacgggg atcgctacca gattaacccg aatcggcatg gagtcaacga cggatgatta
    11701 acgatattaa atgggtacag gcacaacgta aagccaccga ctggcgtcag gccgtggaaa
    11761 tcgccacccg cccgctggta gcctatggtg ccgcgcagcc atgctacgtt aatgggatca
    11821 tcgaaaatac ccttaactgg gggccttatt atctgatagc cccaggaatc gccttgcctc
    11881 acgcccgacc agagcaaggc gcaaactata atcaggtcag tatcaccacg ctacgcaccc
    11941 cggtcgcctt tggtaacgag gagtgcgacc ccgtctggct gcttctctgc gtcagcgcca
    12001 cggatgccaa cgcacatatt ttgaccattc agcgcatcag ccagtttatt gattcgccac
    12061 aacgcttaac ggcagtggga aatgccagca cagacgatgc actgtttgcg ctggtatcag
    12121 gttaacggag gcgcttatga tcctgcaccc ttctctcgcc agcgcaaatc ctctgcacta
    12181 tggtcgtgag ctgacggcat tggacaatct ggattttggg agcctgcatc tggatattga
    12241 ggacagcagc ttcattaaca acattacgtt tgggatgaaa acggtgcagg ccgtcgccag
    12301 acagacgcct catcccttgt cctttcattt catgcttgcc cggccacagc gctggtttaa
    12361 cgcgctggct gaaatcagac ccgcgtggat cttcgttcat gcagaaacgc tggactatcc
    12421 ttcggaaacg ttaactgaga tccgccatac cggcgcacga gccggactgg tatttaatcc
    12481 ggcaaccccg atcgacgcct ggcgatatct ggcgtctgag ctggacggag tgatggtgat
    12541 gaccagtgag cccgatgggc agggacagcg gtttattccc tccatgtgcg agaaaatcca
    12601 aaaagtacgc acggcttttc ctcagaccga gtgctgggcc gacggtggaa ttacgctggc
    12661 agctgcacag caactggcag cagctggggc gcaacatatg gtgattgggc gcgccctgtt
    12721 ctcttcatct gactatcgcg cgaccttagc tcaattcgcc accctgtaag aggagattgt
    12781 ttctgatgag ccaacagaga cctgaccgta tcaaacagat gctgcactac ctgtggcaac
    12841 atcgtcacct ctccacacag caggccatgg agctttttgg ctatgcggaa gcgactgtcc
    12901 gtcgtgattt ccagtatatt gtcaaccagt atccaggcat gatccgcggt cacggttgcc
    12961 tggacttcga cgacagtaca gacgacaaag agtacgtctt cgacgtgaaa cgtacattgc
    13021 aatctgtggc gaaaagagaa atagctgccc ttgcccgaac aatgatcaaa gatggcgact
    13081 gcttttttct cgactccggc tcaacctgcc tcgaactggc gaaatgcctg gccgatgcgc
    13141 gggtaaaggt catctgcaac gacattaaaa tcgccaatga actgggctgt tttccccatg
    13201 tggaaagtta cattatcgga ggactgattc gcccaggtta tttttcggta ggagagagtc
    13261 tggcgctgga gatgatcaat gccttttccg tagaacgtgc gtttatctcc tgcgatgcgc
    13321 tgtcgctgga gacggggatc accaatgcca ccatgtttga ggtcggggtg aaaacacgga
    13381 ttattcaacg ctcgcgtgaa gttattctca tggccgatca ctcaaaattt gatgccgttg
    13441 agccgcatgc tgtagcaaca ctatcatgta ttaaaacgat catcagtgac agcgggttac
    13501 ctgagactat tgcacagcgt tatcagcgag ccggatgtca gttgttttta cctcattcaa
    13561 taaagtgata agtaagttca ttcgagaggg atttcaagca aaaataatca atggcaccca
    13621 atagaaaata ttggcgatgc gctcgaacga ataaagaagc tctaggcgca atccacacac
    13681 tacgatgttg caacaacacg ccatctactt tttattctca ttcactaaat gtggctgttc
    13741 tggattcatt attcaaagtg tgtacaagat cacatttaat cacatcatta cggttcagca
    13801 tgctgaacaa agcatatttt ccactatgta atgccgatac catttattcc atgagcaagg
    13861 aggagccatt atggttcgca aagggtgtaa ttcattagtc cgtgctgaaa aaatattaac
    13921 ccacattgca tgggttggta tggcaagtta tatggagtta ttaaataaat tccagtaccc
    13981 caaaagcagc ctgcttaatt tattgaatgt aatggtggat tgtggtttct taattaaaaa
    14041 caaaaacggc tactattcac tgggaataaa aaactacgaa ttgggttgtc aggcgttaca
    14101 ccgtcaaaat atatttgagg tgactaagcg gccaatgcag gagttgtcgc tgaaaagtgg
    14161 actggtttgt catcttgggg cgatggagag catttcggct atctatctcg acaagatcga
    14221 aagtccggac tctgtaccga cgagcaaaag ctggattggc aaaaaacttg agttgcatat
    14281 taccgcgttg ggtaaggcat tattagcctg gaagacacga gaagaactgg attatttttt
    14341 agaagcactc acgttaactc cacatacgcg caatacattt accgataaaa aattgtttct
    14401 ggaagaatta caaaaaacac gccttcgggg atgggccata gacaacgaag aatcaaccta
    14461 tggggcggta tgtttaagta tgccggtatt caatatgtat aacagagtta actatgcaat
    14521 ctcactttct ggcgatccgg tagtttattc aggaaataag atagacagct atctggaatt
    14581 gctccggaaa tgcgctgagc aaatatcata tgggctggga tacagaaacg aaaatgagca
    14641 cttacgaaaa ggaaactgag gtaatgaaaa aattcagcgg cattattcca ccggtatcca
    14701 gcacgtttca tcgtgacgga acccttgata aaaaggcaat gcgcgaagtt gccgacttcc
    14761 tgattaataa aggggtcgac gggctgtttt atctgggtac cggtggtgaa tttagccaaa
    14821 tgaatacagc ccagcgcatg acactcgccg aagaagctgt aaccattgtc gacgggcgag
    14881 tgccggtatt gattggcgtc ggttcccctt ccactgacga agcggtcaaa ctggcgcagc
    14941 atgcgcaagc ctacggcgct gatggtatcg tcgccatcaa cccctactac tggaaagtcg
    15001 caccacgaaa tcttgacgac tattaccagc agatcgcccg tagcatcacc ctaccggtga
    15061 tcctgtacaa ctttccggat ctgacgggtc aggacttaac cccggaaacc gtgacgcgtc
    15121 tggctctgca aaacgagaat atcgttggca tcaaagacac catcgacagc gttggtcact
    15181 tgcgtacgat gatcaacaca gttaagtcgg tacgcccgtc gttttcggta ttctgcggtt
    15241 acgatgatca tttgctgaat acgatgctgc tgggcggcga cggtgcgata accgccagcg
    15301 ctaactttgc tccggaactc tccgtcggca tctaccgcgc ctggcgtgaa ggcgatctgg
    15361 cgaccgctgc gacgctgaat aaaaaactac tacaactgcc cgctatttac gccctcgaaa
    15421 caccgtttgt ctcactgatc aaatacagca tgcagtgtgt cgggctgcct gtagagacat
    15481 attgcttacc accgattctt gaagcatctg aagaagcaaa agataaagtc cacgtgctgc
    15541 ttaccgcgca gggcatttta ccagtctgag gagaaactca tgtctgttcg caatattttt
    15601 gctgacgaga gccacgatat ttacaccgtc agaacgcacg ccgatggccc ggacggcgaa
    15661 ctcccattaa ccgcagagat gcttatcaac cgcccgagcg gggatctgtt cggtatgacc
    15721 atgaatgccg gaatgggttg gtctccggac gagctggatc gggacggtat tttactgctc
    15781 agtacactcg gtggcttacg cggcgcagac ggtaaacccg tggcgctggc gttgcaccag
    15841 gggcattacg aactggacat ccagatgaaa gcggcggccg aggttattaa agccaaccat
    15901 gccctgccct atgccgtgta cgtctccgat ccttgtgacg ggcgtactca gggtacaacg
    15961 gggatgtttg attcgctacc ataccgaaat gacgcatcga tggtaatgcg ccgccttatt
    16021 cgctctctgc ccgacgcgaa agcagttatt ggtgtggcga gttgcgataa ggggcttccg
    16081 gccaccatga tggcactcgc cgcgcagcac aacatcgcaa ccgtgctggt ccccggcggc
    16141 gcgacgctgc ccgcaaagga tggagaagac aacggcaagg tgcaaaccat tggcgcacgc
    16201 ttcgccaatg gcgaattatc tctacaggac gcacgccgtg cgggctgtaa agcctgtgcc
    16261 tcttccggcg gcggctgtca atttttgggc actgccggga catctcaggt ggtggccgaa
    16321 ggattgggac tggcaatccc acattcagcc ctggcccctt ccggtgagcc tgtgtggcgg
    16381 gagatcgcca gagcttccgc gcgagctgcg ctgaacctga gtcaaaaagg catcaccacc
    16441 cgggaaattc tcaccgataa agcgatagag aatgcgatga cggtccatgc cgcgttcggt
    16501 ggttcaacaa acctgctgtt acacatcccg gcaattgctc accaggcagg ttgccatatc
    16561 ccgaccgttg atgactggat ccgcatcaac aagcgcgtgc cccgactggt gagcgtactg
    16621 cctaatggcc cggtttatca tccaacggtc aatgccttta tggcaggtgg tgtgccggaa
    16681 gtcatgttgc atctgcgcag cctcggattg ttgcatgaag acgttatgac ggttaccggc
    16741 agcacgctga aagaaaacct cgactggtgg gagcactccg aacggcgtca gcggttcaag
    16801 caactcctgc tcgatcagga acaaatcaac gctgacgaag tgatcatgtc tccgcagcaa
    16861 gcaaaagcgc gcggattaac ctcaactatc accttcccgg tgggcaatat tgcgccagaa
    16921 ggttcggtga tcaaatccac cgccattgac ccctcgatga ttgatgagca aggtatctat
    16981 taccataaag gtgtggcgaa ggtttatctg tccgagaaaa gtgcgattta cgatatcaaa
    17041 catgacaaga tcaaggcggg cgatattctg gtcattattg gcgttggacc ttcaggtaca
    17101 gggatggaag aaacctacca ggttaccagt gccctgaagc atctgtcata cggtaagcat
    17161 gtttcgttaa tcaccgatgc acgtttctcg ggcgtttcta ctggcgcgtg catcggccat
    17221 gtggggccag aagcgctggc cggaggcccc atcggtaaat tacgcaccgg ggatttaatt
    17281 gaaattaaaa ttgattgtcg cgagcttcac ggcgaagtca atttcctcgg aacccgtagc
    17341 gatgaacaat taccttcaca ggaggaggca actgcaatat taaatgccag acccagccat
    17401 caggatttac ttcccgatcc tgaattgcca gatgataccc ggctatgggc aatgcttcag
    17461 gccgtgagtg gtgggacatg gaccggttgt atttatgatg taaacaaaat tggcgcggct
    17521 ttgcgcgatt ttatgaataa aaactgaaag cataaataaa aacatccaga cacctgaatt
    17581 atccgactct gcatcattcg ttatttgctc agtcggataa tgatgggtcg ctaccctgtt
    17641 atgagaggac gttatgccac taattatcgt tgtggcaggg attgctttac tcctgctttt
    17701 aaccataaaa attaagctta atacgtttgt ttcgttaatt attgtctcga ttgctgtcgc
    17761 catcgccagt ggtatggatt tgagtaaagt cgtcacctca gtcgaatccg gacttggcgg
    17821 tacgctgggc catattggtt tgatatttgg cttcggtgtt atgctcggtc gcttgctggc
    17881 cgatgcgggt ggtgcgcagc gaatagcgct gaccatgctg aattatttcg ggaaaaataa
    17941 acttgactgg gcagtggtct gttcggcgtt tattgtcggt atcgcacttt ttttcgaagt
    18001 cggtttaatt cttctggtac ctattttatt cgctattgcc cgtgaagcaa aaatatcacc
    18061 gatgtttatg tgtgtgccga tgctttctgg tttgctggtc gcacacggtt ttttgccgcc
    18121 gcaccccggc ccgactgtta tcgccaggga atatggcgct gatgtaggat tggtactgat
    18181 atacggtatc attgtcggca ttccaacctt tattctttgc ggcccggtat tgaataaatt
    18241 ctgccaacgc attattccgg acgcctttaa aaaagagggg aatatagcct ctctcggcgc
    18301 gacccgccga ttcagcgaaa gcgaaatgcc aggctttggg atcagttttc tcacggcaat
    18361 gctgccggtg atcctgatgg cggttgtcac catcatccag atgactcatg ccaaaagtgc
    18421 cgcggattca ggcttatttt acaacgtgat actgttctta ggtaattcaa cgatcgccat
    18481 gctgatttca ctgctattcg cgatctatac catgggcctg gggcgcggga aaacaatccc
    18541 tgacctgatg gattcttgcg gaaaagctat cgcgggtatt gccggtctgc tgctgattat
    18601 cggtggtggt ggtgcattca agcaagttct catcgactct ggcgttggtc agtatatttc
    18661 caccctggtt tcgggtatgg atattaaccc gatcctgatg gcctggggcg tggccgcatt
    18721 cctgcgtatt tgtctgggtt cagccaccgt cgcggcgatc tctaccgccg gtctggtcat
    18781 cccattgttg gcagtacatc ccaataccaa cctggccttg attacgctgg ctaccggtgc
    18841 cggatcctgt atttgttctc acgttaacga tgccagtttc tggatgatta aagacttttt
    18901 tggcctgacc accaaagaaa cgctgttatc ctggacgctg atgtccacac tcttatccat
    18961 cagtgggctg atttttattc tgctcgccag cttagtttta taatcgggtt cttgccagtg
    19021 agccaacctg ttgttcactg tcccccataa tttagtgagt gacggcaacc gtctgcacgg
    19081 tgacgaatcg ctggcgatca aaaaggggcg atattgaacc ttcgattatg cttgatgggt
    19141 gtattctcag taatactgta aagccggagc atgtctccgg ctttacagta tgggggaaca
    19201 gcgggcgatc gctcaaatgg tatgctggtt tatttcatgt aaaagaataa aatatctcta
    19261 taaaatcatg ccactggcaa tctgcatcaa ctctccgggt aaacagggtt aagatgagca
    19321 tttccccggt ttaatacagg tatacaaaag tggatagaga tcaaacaaac agcagcctgt
    19381 tcaatgatga tcccgtactt catgcaacat ggctgtacta tcaggaaggt aaaagccaga
    19441 ctgaagttgc agctatcatg ggggtgtccc gcgtcacggt tgtcaaatac ctgcaaacag
    19501 cccgggaaaa tggactggtt catatcaacc tggatgtgaa tgtctttggc tctatcgacg
    19561 ctgctttgca aattcgcgat aaattcaatc tccagagagt gatcatcgtg ccggatggcg
    19621 aacatgccgg aaaacgtgat gatacaaagc tgatgcggac tcgcctttca cgtgccggtg
    19681 gcatgtatct gaatcaggtg attgagaatg gtgatgtgct gggggttgcc tggggaagaa
    19741 caatccatca gatgagcaaa accatgacgc cgaagtcatg taaaaatgtc accgttatac
    19801 agatgctcgg ttccatgcca tctcagccag acctgacgat cattgaatcc tcatcgcaaa
    19861 tcgcctacaa gctttcaggc cgtgtcgcct cgctgcacgt gcccgccgtg gtatcaagcg
    19921 ccagactggc catggagtta caggcagagc ctattattcg atcgaatttt gacgttctga
    19981 ctcgctgtac gaaagccttt tttgtcgtgg gtaacgcgct tgatgaaaat cccctgattc
    20041 gggtgggcgt gctgaataag aaggaaatgc agacgtatcg ggatttaggc gcagtaggtg
    20101 tcatttgtgg tcgcttctat gacaaggaag ggatgcccgt cgtggctgat gttgaccagc
    20161 gtattctggg aatcagtctg gctcagctaa ggcagattga acggaagatc ttccttgctg
    20221 gtggggaaag aggctatgac gcaacgttgg gtgccctgct gggaggatat gtcacggacc
    20281 tgattgttga tgaaggaacg gcagagtttt tgctggcgtg tgaattacca cactaaaaga
    20341 atgaacaact gccccatgta gatatggggc aggattttat tgattagcgg cgataataag
    20401 aacaataccc acaatgtatg aaataaacat ggtcagcagc agtttattgg ttccggctgg
    20461 cgcactggca aattctctcc agatgaagac tccccagatc acggcaatca ttgtggcccc
    20521 ctgtcccaga ccgtaggaaa tggcataccc cgcctgacca gacgcaatca gactgaatgc
    20581 cagaccgaca caccaaatca taccgcccag ccagccgcaa acatgatccc gtaaactccc
    20641 ggaaaaatac attttcccgt tgaccggttc tcctgaaata ggttttttca tgaccagcct
    20701 gttaaggaca aagttcgaca aaaacagccc cacggcaaac agaacaagtg ctgagtaagg
    20761 cgtcatcaga ccgctggcag gctggctgaa attgtcagac atggagtccg ccaggaagcg
    20821 gaaaaaccat cccatgatga tacccgccag aatggcagta atgagacctt tattacggcg
    20881 agatttatcc gcctcctgcg tgacacgacc ataggctatc gcggtaaaaa taatggcgat
    20941 cacaacgcag acgacaccga ggaacagaat gagcagatcg ccctgcggat tgccgatata
    21001 ggtggtgatc acacccagag ccagtgccag ccccacaccc acaggaaaag ctaccgccat
    21061 accggcaaga ttaatagaag cgaccagaag aatattggaa atattgaaga taatccctgc
    21121 cagtattgcc gacatcagac tactgctgct cgcctgctga atatcaggta taaaactgcg
    21181 accggcttcg cccatagaac ccagcgtgaa ggcaaataac aaagaacaca gcaataatcc
    21241 aattgaataa tcccaataga atagagggaa ctcccatttt tattgctgac aagtttagtt
    21301 gtattcgccc atgaacccca gcaaatcatg gtaataaaac acattattat ggcgacagca
    21361 tacgattcca cgataaacat aaataactcc ttgtggtagt tataatcaat cgttgtggac
    21421 agagggaata gcagccattc tgcgaatggc tgtcatatgt tattaattaa aataaagctg
    21481 taattacgct ttcaaaccat acgttttgaa tttttcaagg acgatggtca tttcggcttc
    21541 agacagcacg ggaacatcgt gatgtaatcc gccacatttg atgtataaat cagccagcgt
    21601 ctcggtttca cccgccagcc acaacgtctt ttccagcgtg acgtccatgg ccagcatacc
    21661 gtggtgctgc atcagcaagg acttactttc tctgattcct ttagatacac cgtcggccag
    21721 ttcaggactg ccaaaggtat aatagggaat acagggaata tgatccgtcc ccgataccgc
    21781 caccatataa tgaatggccg gaattgaatg attatgtatt gccaccgccg tggaattcac
    21841 cgcatgcgta tgtatgacag cattcaattc tggtctggct ttataacacg ccagatgaat
    21901 taaccattca cttgatggta ttttacccgc ttcaggtttt cccttatcgt ctacaaagac
    21961 aatcatgtcc ggcgtcatct ttgaataagc gatcccactc ggcgtaatca acatgccacc
    22021 ggtatacctg gcactcacat tacctgatgt tccttgatta agacctgacg cattcatttc
    22081 ccgacaagtg gaaatgattt tttctgctaa cttaatccgt tccattcctg atccttaata
    22141 gcaatggccg ctgttttaat gctgttcccc ctggagtgct ttactcttca ggtcagcaaa
    22201 catatccgcc agcgctgact tactggcagc acagactccc cgctcagtaa tgaagccggt
    22261 aatgtatcgg gcaggcgtga cgtcgaaagc gtagttccca caacgggttc cttcaggggc
    22321 ggtattgacc cagctcaatt ctccctgagg gtttatccca tagacatggg actgctcttt
    22381 tccgtcacgt tgctcaatcg gaatgctttt tccgtcctcg atagtccagt caatagtggg
    22441 agaaggtaac gcgacgtaga agggaacatg gttatcatgg gccgccagcg ccttaagata
    22501 ggtaccaatt ttattacaga catcccctct ggccgtggtt cgatcggtcc caacaatgca
    22561 gagatcaacg tcaccgtgtt gcatcaggtg gccacccgcg ttgtcggcga tcagggtgtg
    22621 aggaataccg tgtgagccca gttcaaatgc cgtgagtccg ccctgattac gtggccgcgt
    22681 ttcatccacc cagacgtgaa cagggattcc gttttcatgc gctttgtaga tgggagaaag
    22741 cgcggttccc cagtctacag tcgccagcca gcctgcgtta caatgtgtca ggatattgac
    22801 cacactcccg gccggttttt tctgggctat ttcgcggatg acttctgcac catgtaatcc
    22861 aatttgttca cacaacgcga catcttcctc ggcaatttct gccgccagcg cccaggccac
    22921 atccttgcgc tctgatacac agagatcctt cagtgtgtct atcatcctgt cgagggccca
    22981 tttgagatta attgccgttg gtcgggtttt gataagaagg tcatagtaac gctgcagacc
    23041 catgtcactg gcatcgtgat tcattcccag cgcgatgcca taggctgcaa cgacaccaat
    23101 aagcggagcg ccacgaaccc acatatcctg aatagccgta gcggccattt ccgcagaggt
    23161 aagcgccacc acctcgaact taaaaggcaa cttagtctga tcgatgattt ctaccgcttt
    23221 tccatccccg gaaacccaga ccgtacgata gtgtttacct ttaatattca taatgacctc
    23281 ttacttaacc ttcgcagact ggatgacgga atccatattt ttaaactggc gggcattgac
    23341 gataagatcg cgcgccatgg tcaatgcacg tcgttcacat cctgcgcgaa gctctgtatt
    23401 ttcaatctgt ttcagctcgg caacgccagc aaaaccaatg atccggcgat tcatttccat
    23461 gccggcattc accagggaat cttcaagcag cgtggcaaaa aactgatcct gtgcgtcctc
    23521 aagagcggcc ctgttctgtt gatacatttc tgtcgaccac gcgtcgccct gtgttttgtt
    23581 gtcccagagc tggcggaact cccgggtgaa aacggaccag gtttggacaa tctgctgaag
    23641 cagccattcc tgatagtcag cgcgacgttg ctcattcgca tcccacccag ggcgtgagaa
    23701 gtacgccagc aggaggttgc caatatagtt gccgatatca aacgccattg gccccatgaa
    23761 accgaactcc ggatcaatca ctttggtatc cgtgtcggtc accatgattg agcctgaatg
    23821 aaggtcgcca tgtaacaatg cctgcgcttc tgtcataaat ttgtacttat aacgcatggc
    23881 aacctggatc atctctacat cagcccaggc cttatggacg gcatcgtcca gctcaggaga
    23941 agtccagtta ttccgttcag cgttgtaata gggctctgtg aagatcagat cttccgtaat
    24001 tttgcacagc tcatgattca acgcgaactc ggcagtaagc gcttttttct gttctgctga
    24061 catgccaatg tcagacgtat tgaagagagt ctgtgctaaa aatctgccga tatcttcagc
    24121 tagtttaggg aattttttac cgttaatcag ttccttacgc agaatcacgt gaggagtcag
    24181 atactccatg gcaaacagcg ccatttccgg gtcgtaaaaa tagacctgcg gaatcagtgt
    24241 gtgacccgcg tacttggctt cctgacataa gacgttgtac tcaaagcccg cacggctcag
    24301 agataaaggc catccttcgc ccccggcacg aagccagggc agagcttgtt ttacaatgat
    24361 ggttttatgt gtcccctcaa cgataaacac catgttcagg tttccgtccc ccacttctga
    24421 aactttccac tcttcaggta accctcccgg ttcgcatgaa ggggtcagtc tggacgagag
    24481 ataacccggc agcgtgtcac atgtcagcgg cttgtaacct gatggaattg aatccgtcat
    24541 aacagttctc cttgtctaca cagaatgata atcaacttgt catgtgacaa ctatacatta
    24601 tcaaatgtaa aattatttg
//
LOCUS       DAPHJE010000066        24473 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPHJE010000066 DAPHJE010000000
VERSION     DAPHJE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.066"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            105..866
                     /gene="adiY"
                     /locus_tag="RH222_002698"
     CDS             105..866
                     /gene="adiY"
                     /locus_tag="RH222_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV3797691.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1003..2340
                     /gene="adiC"
                     /locus_tag="RH222_002699"
     CDS             1003..2340
                     /gene="adiC"
                     /locus_tag="RH222_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV3797692.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2444..4087
                     /gene="eptA"
                     /locus_tag="RH222_002700"
     CDS             2444..4087
                     /gene="eptA"
                     /locus_tag="RH222_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV3797693.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4084..4752
                     /gene="basR"
                     /locus_tag="RH222_002701"
     CDS             4084..4752
                     /gene="basR"
                     /locus_tag="RH222_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV3797694.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4753..5853
                     /gene="pmrB"
                     /locus_tag="RH222_002702"
     CDS             4753..5853
                     /gene="pmrB"
                     /locus_tag="RH222_002702"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV3797695.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5829..5918)
                     /gene="pmrR"
                     /locus_tag="RH222_002703"
     CDS             complement(5829..5918)
                     /gene="pmrR"
                     /locus_tag="RH222_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV3797696.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6030..7532)
                     /gene="proP"
                     /locus_tag="RH222_002704"
     CDS             complement(6030..7532)
                     /gene="proP"
                     /locus_tag="RH222_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV3797697.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7796..8674)
                     /locus_tag="RH222_002705"
     CDS             complement(7796..8674)
                     /locus_tag="RH222_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV3797698.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8671..10899)
                     /gene="crfC"
                     /locus_tag="RH222_002706"
     CDS             complement(8671..10899)
                     /gene="crfC"
                     /locus_tag="RH222_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV3797699.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11302..11637
                     /locus_tag="RH222_002707"
     CDS             11302..11637
                     /locus_tag="RH222_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV3797700.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11797..12240
                     /gene="yjdN"
                     /locus_tag="RH222_002708"
     CDS             11797..12240
                     /gene="yjdN"
                     /locus_tag="RH222_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV3797701.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12373..13161
                     /gene="phnC"
                     /locus_tag="RH222_002709"
     CDS             12373..13161
                     /gene="phnC"
                     /locus_tag="RH222_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV3797702.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            13186..14202
                     /gene="phnD"
                     /locus_tag="RH222_002710"
     CDS             13186..14202
                     /gene="phnD"
                     /locus_tag="RH222_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3797703.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14308..15087
                     /gene="phnE"
                     /locus_tag="RH222_002711"
     CDS             14308..15087
                     /gene="phnE"
                     /locus_tag="RH222_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV3797704.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15108..15833
                     /gene="phnF"
                     /locus_tag="RH222_002712"
     CDS             15108..15833
                     /gene="phnF"
                     /locus_tag="RH222_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV3797705.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15834..16286
                     /gene="phnG"
                     /locus_tag="RH222_002713"
     CDS             15834..16286
                     /gene="phnG"
                     /locus_tag="RH222_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV3797706.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16283..16867
                     /gene="phnH"
                     /locus_tag="RH222_002714"
     CDS             16283..16867
                     /gene="phnH"
                     /locus_tag="RH222_002714"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV3797707.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            16867..17931
                     /gene="phnI"
                     /locus_tag="RH222_002715"
     CDS             16867..17931
                     /gene="phnI"
                     /locus_tag="RH222_002715"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV3797708.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSIAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            17924..18769
                     /gene="phnJ"
                     /locus_tag="RH222_002716"
     CDS             17924..18769
                     /gene="phnJ"
                     /locus_tag="RH222_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV3797709.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            18766..19524
                     /gene="phnK"
                     /locus_tag="RH222_002717"
     CDS             18766..19524
                     /gene="phnK"
                     /locus_tag="RH222_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV3797710.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19701..20381
                     /gene="phnL"
                     /locus_tag="RH222_002718"
     CDS             19701..20381
                     /gene="phnL"
                     /locus_tag="RH222_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV3797711.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            20378..21514
                     /gene="phnM"
                     /locus_tag="RH222_002719"
     CDS             20378..21514
                     /gene="phnM"
                     /locus_tag="RH222_002719"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV3797712.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            21514..22071
                     /gene="phnN"
                     /locus_tag="RH222_002720"
     CDS             21514..22071
                     /gene="phnN"
                     /locus_tag="RH222_002720"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV3797713.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            22058..22492
                     /gene="phnO"
                     /locus_tag="RH222_002721"
     CDS             22058..22492
                     /gene="phnO"
                     /locus_tag="RH222_002721"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV3797714.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            22494..23252
                     /gene="phnP"
                     /locus_tag="RH222_002722"
     CDS             22494..23252
                     /gene="phnP"
                     /locus_tag="RH222_002722"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV3797715.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(23399..23728)
                     /gene="yjdP"
                     /locus_tag="RH222_002723"
     CDS             complement(23399..23728)
                     /gene="yjdP"
                     /locus_tag="RH222_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDV3797716.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23797..24246)
                     /gene="rpiB"
                     /locus_tag="RH222_002724"
     CDS             complement(23797..24246)
                     /gene="rpiB"
                     /locus_tag="RH222_002724"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDV3797717.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
BASE COUNT         5786 a         6897 c         6416 g         5374 t
ORIGIN      
        1 atttttatgc atgtttatat tcatcataca attatataac catttcccgg tatcgctttg
       61 ctttagcgag aactggtctt ttatttgcac tcaggagtac atgtatgagg atttgcagcg
      121 accaaccttg tattgtttta ttgactgaaa aagatgtctg gataagggtg aatgggaaag
      181 aacctattag ccttaaagct aaccatatgg cgttattaaa ttgtgaaaat aatattatcg
      241 acgtctcctc tcttaacaac actttggttg ctcatattag tcacgacatc atcaaagatt
      301 acctccggtt tctgaataaa gatctctcgc aaataccagt atggcaacgt agcgctacgc
      361 ccatactcac cctgccatgc ctgacgccag acgtctttcg cgttgccgcg caacacagca
      421 tgatgcccgc agaaactgag tcagaaaagg aacgaacacg cgcattatta ttcacggtgc
      481 tatcccgttt tctcgacagt aaaaaattcc tttcattaat gatgtatatg ttacgtaatt
      541 gtgtaagtga cagcgtttat caaattattg aaagcgatat tcacaaagac tggaatctta
      601 gtatggtagc cagttgttta tgtcttagcc caagtctgtt aaagaaaaag ctgaaaagcg
      661 aaaacaccag ttatagccaa ataatcacca cctgccgcat gcgttatgcc gtaaatgaat
      721 taatgatgga cggtaaaaat atctctcagg tatcacagtc ctgcggctac aacagtacgt
      781 cgtactttat ttctgtcttt aaagacttct acggtatgac gccgctgcat tatgttagtc
      841 agcacagaga acgcactgtc gcctgatttt taaccttaac gaagagctat attaataacg
      901 gcatcagcga taacccggtc gataataatt caactatcga atgcaggcgt atgatatgac
      961 gtaatttatt gtcacgaagc tcgccttcgc aggagtttaa ttatgtcttc ggatgctgat
     1021 gctcacaaag tgggcttaat ccccgtcacc ctgatggtgt cggggaatat tatggggtca
     1081 ggtgtttttc tgttacctgc aaacctggcc tcgactggcg ggattgccat ttatggatgg
     1141 ttggtgacga ttatcggtgc gctggggctc tcgatggtat acgccaaaat gtcgttcctc
     1201 gatccaagtc ctggtggttc ttacgcttac gcccgccgct gctttggccc gtttctcggt
     1261 tatcaaacca acgtcctcta ctggctggcc tgctggatcg gcaatatcgc catggtggtc
     1321 attggcgtag gatatttaag ttacttcttc ccgattctga aagatccatt ggtattaacc
     1381 atcacctgcg tcgtggtgct gtggatcttc gtcctgctga acattgtcgg tccgaaaatg
     1441 atcacccgtg tgcaggcagt tgccaccgtg ctggcgctga tccccatcgt cgggattgcc
     1501 gtatttggct ggttctggtt ccgtggtgaa acctatatgg cggcatggaa cgtcagcggc
     1561 ctgggcacct tcggtgcaat tcaaagtacc cttaacgtta cgctgtggtc gttcatcggt
     1621 gtggaaagtg cctccgttgc cgcaggtgtg gtgaaaaacc cgaaacgcaa tgtccctatc
     1681 gccaccattg gtggggtatt gattgccgcc gtttgctatg tactttctac caccgcgatt
     1741 atggggatga tccctaatgc cgcactgcgc gtttctgctt cgccattcgg tgatgccgca
     1801 cggatggcgt tgggtgacac cgccggggcc attgtttcct tctgcgcagc tgcgggttgc
     1861 ttaggttcac tgggcggctg gacgttgctg gcgggtcaaa cggcgaaagc cgctgccgat
     1921 gacggactgt tcccaccaat ttttgcccgt gtaaataaag cgggtacgcc agtggcgggg
     1981 ctgattatcg tcggtatttt gatgaccatc ttccagctca gcagcatttc accaaacgcg
     2041 accaaagagt tcggtctggt ttcttccgtc tcggtcatct ttacactggt gccatatctt
     2101 tacacctgtg cggcgttact gctgctcgga cacggtcact ttggtaaagc acgcccggca
     2161 tatctggcag ttactaccat tgccttcctc tactgcatct gggccgtggt ggggtccgga
     2221 gcgaaagagg ttatgtggtc atttgtcacc ctgatggtca tcaccgccat gtatgcgctg
     2281 aattacaacc ggctacataa aaacccgtat cccttagatg caccaataag caaagattaa
     2341 ttccccttaa tccagcaaac ataaaagcca accttaagaa cttaaggttg gcttaatttt
     2401 gctttgcgag catatgcgca ctttgttcga tggaaacacc gtgatgttga agcgcctact
     2461 aaaaagaccc tctttgaatt tactcgcctg gctattgttg gccgcttttt atatctctat
     2521 ctgcctgaat attgcctttt ttaaacaggt gttgcaggcg ctgccgctgg attcgctgca
     2581 taacgtactg gttttcttgt cgatgccggt cgtcgctttc agcgtgatta atattgtcct
     2641 gacactaagc tctttcttat ggcttaatcg accactggcc tgcctgttta ttctggttgg
     2701 cgcggctgca caatatttca taatgactta cggcatcgtc atcgaccgct cgatgattgc
     2761 caatattatt gataccactc cggcagaaag ttatgcgctg atgacaccgc aaatgttatt
     2821 aacgctggga ttcagcggcg tgcttgctgc gctgattgcc tgctggataa aaatcaaacc
     2881 tgccacctcg cgtctgcgca gtgttctttt ccgtggagcc aatattctgg tttctgtact
     2941 actgattttg ctggtcgccg cactgtttta taaagactac gcctcgttgt tccgcaataa
     3001 caaagagctg gtgaaatcct taagcccctc taacagcatt gttgccagct ggtcatggta
     3061 ctcccatcag cgactggcaa atctaccgct ggtgcgaatt ggtgaagacg cccaccgcaa
     3121 cccgttaatg cagaacgaaa aacgtaaaaa tttgaccatc ctgattgtcg gcgaaacctc
     3181 gcgggcggag aacttctccc tcaacggcta cccgcgtgaa actaacccgc ggctggcgaa
     3241 agataacgtg gtctatttcc ctaataccgc atcttgcggc acggcaacgg cagtttcagt
     3301 accgtgcatg ttctcggata tgccgcgtga gcattacaaa gaagagctgg cacagcacca
     3361 ggaaggcgtg ctggatatca ttcagcgagc gggcatcaac gtgctgtgga atgacaacga
     3421 tggcggctgt aaaggtgcct gcgaccgcgt gcctcaccag aacgtcaccg cgctgaatct
     3481 gcctggtcag tgcatcaacg gcgaatgcta tgacgaagtg ctgttccacg ggctggaaga
     3541 gtacatcaat aacctgcagg gtgatggcgt gattgtctta cacaccatcg gcagccacgg
     3601 tccgacctat tacaaccgct atccgccgca gttcaggaaa tttaccccaa cctgcgacac
     3661 caatgagatc cagacctgta gcaaagagca actggtgaac acttacgaca acacgctggt
     3721 ttacgtcgac tatattgttg ataaagcgat taatctgctg aaagaacatc aggataaatt
     3781 taccaccagc ctggtttatc tttctgacca cggtgaatcg ttaggtgaaa atggcatcta
     3841 tctgcacggt ctgccttatg ccatcgcccc ggatagccaa aaacaggtgc caatgctgct
     3901 gtggctgtcg gaggattatc aaaaacggta tcaggttgac cagaactgcc tgcaaaaaca
     3961 ggcgcaaacg caacactatt cacaagacaa tttattctcc acgctattgg gattaactgg
     4021 cgttgagacg aagtattacc aggctgcgga tgatattctg caaacttgca ggagagtgag
     4081 tgaatgaaaa ttctgattgt tgaagacgat acgctgttat tgcagggatt gattctggcg
     4141 gcgcaaaccg aaggctacgc gtgcgatggc gtgacaaccg cgcggatggc ggaacaaagc
     4201 cttgaggccg gtcattacag cctggtggta ctggatttag ggttacccga cgaagatgga
     4261 ctgcattttc tcgcccgtat ccggcagaaa aaatacaccc tgccagtact gatcctcacc
     4321 gctcgcgata cgctgaccga caaaatcgcc gggctggatg tcggtgccga tgactatctg
     4381 gtgaagcctt ttgcgctgga agagttacac gcccgtatcc gcgccctgct acgacgccat
     4441 aataatcagg gcgaaagtga gctgattgtt ggcaatctga cgctgaatat gggtcgccgt
     4501 caggtatgga tgggcggtga agagttgatt ctgacgccca aagaatatgc tctgctgtca
     4561 cggttaatgc tcaaagcagg cagtccggta catcgggaaa ttctctacaa cgacatctat
     4621 aactgggaca atgaaccctc gaccaacacc ctggaagtgc atatccacaa tctgcgcgac
     4681 aaagtgggca aagcccgtat ccgcaccgtg cgcggctttg gctatatgct ggtcgcgaat
     4741 gaggaaaact aattgaatct gatgcatttt ctgcgccgac caatatcgct gcgccaacgg
     4801 ctgatattga ccatcggggc cattttgttg gtgtttgagc tgatcagcgt cttctggcta
     4861 tggcatgaaa gtaccgagca gattcagctg tttgagcagg cgctacgcga caatcgcaac
     4921 aacgatcgcc acatcatgcg tgagatccgc gaggcggtcg ccagcctgat tgtccccggc
     4981 gtctttatgg tcagcctgac gctatttatc tgctatcagg cggtgcgccg catcacccgc
     5041 ccgctggcgg agctgcaaaa agagctggaa gcgcgcactg ccgacaactt aacgcccatt
     5101 gccattcaca gcgccaccct cgaaatcgaa gcggtggttt cggcgttaaa cgatctggtc
     5161 agtcgcctga ccagcacgct ggataacgaa aggttgttta ccgctgacgt cgcgcacgaa
     5221 ctgcgaacgc cactggcggg ggtgcgtttg catctggaac tgctggcgaa aacgcatcac
     5281 attgatgtag caccgttagt ggcacgactt gatcagatga tggagagcgt ctcccagctg
     5341 ctgcaactgg cgcgtgccgg acagtcattt tcttccggta attatcaaca tgtaaaactg
     5401 ctggaagatg tcattctccc ctcgtatgac gaactcagta ccatgctcga ccagcgacag
     5461 caaaccctgc tattgccaga gagcgccgcc gacatcaccg ttcagggtga tgcaaccctg
     5521 ctgcggatgt tattgcgaaa cctggtagaa aacgcccatc gttacagccc gcaaggcagc
     5581 aacattatga ttaagctgca agaagatggc ggagcggtca tggcagttga agatgaagga
     5641 ccaggtattg atgagagtaa atgcggggag ttgagtaaag cgtttgtacg tatggacagc
     5701 cgttatggcg ggattggtct ggggttaagt attgtcagcc gcattacaca gttgcatcac
     5761 gggcagtttt tcctgcaaaa ccggcaagag acttccggca cgcgggcctg ggtacggctg
     5821 aagaaagatc agaacgtggc aaaccagata taaagaaagc tgctgaccac cagcacgctg
     5881 aacacggtag ttaaactttc ataaacacgg tttttcattg cactctcctc cgttaacctg
     5941 gaggagagta tgcgcgtcag agattaagtg aaccttaaga gttcaattgg cctgataaga
     6001 cagcgtcaca tcaggccatc cgtttcagct tattcatcaa ttcgcggatg ttgctgcacc
     6061 aggcgggtac gtttcgcctg caaatcggca atctcgtggt caatattatc gattttctgc
     6121 tcgatattat cgtaatgctc gacgagaatt tccttcgctt cctgtatatc tgacgccgcc
     6181 ggtgtcgcac ctttcaacgg acgatttgcc gtctctttca tggttacgcc ggtgattaaa
     6241 ccaatcaccg ccactaccat caggtaatag gcaggcatca tcagattctg cgagctttcg
     6301 accagccagg ccgccagtgt tggcgtcaga ccggcaacca gcaccgaaat attaaatgcc
     6361 gccgccagcg cgctgtaacg gatatgcgtc gggaacatcg ctggcaaggt agaagccata
     6421 acgcccgtaa agcagttaag gatcaccgcc agcatcagta acccggcaaa aatcaggccg
     6481 atgacgttac tgttaatcag aataaacgcc gggatcgcca acacaaacag ggcaacacta
     6541 ccaagtagca caaacggacg acggccaaaa cggtcactca gcaagcccat caccggctgg
     6601 acaaacagca taccgatcat aatggcgata ataatcagca ccccgtggtc ttcggagtaa
     6661 tgcaggttat gcgacaaata actcggcata taggtcagca acatgtagta agtcacgttg
     6721 gtggcaatta ccagaccaat acatgtcaac aggctgcgcc agtgtttggt ggcaatctct
     6781 ttaaacgaga ctttcgggcc atcctgcaaa ccttcgcggt cgccctgttc cagtttatcg
     6841 acatgctgct ggaacgccgg agtttcttcc agcgcatggc gcaggtaaag cccgataatc
     6901 cctaacggca gagcaataaa gaacggaata cgccagcccc agtcgaggaa gttcgcttcg
     6961 ccgacaatgg tcgaaattaa caccactacg ccagcaccca gcacaaaccc ggcaatagaa
     7021 ccaaagtcca gccagctgcc cataaagcca cgtttacggt caggggagta ttccgcaaca
     7081 aatatcgacg ccccggtata ttcaccgccg accgagaaac cttgtgccat cttacagatc
     7141 aacagcagaa tcggtgccca aataccaatc gtgtcgtagg acggtattaa gccaatacag
     7201 aacgtactga tcgacataat cacaatagtg atagcgagga tcttctggcg accatattta
     7261 tcgcccaaca taccaaagaa gagtccgcca agcggtcgaa tcagaaaggg aacggagaac
     7321 gtggcaagtg cagcaaccat ctgcacgctg gggtcagccc ccgggaaaaa aactttacct
     7381 aatgcgtaag caacaaaacc ataaacacca aaatcaaacc attccattgc attacccagt
     7441 gatgctgcgg taatggcttt acgcagttta ccgtcatcaa taatggtgac atcacgaagg
     7501 gtaatcggtt ttactttttt ccttttcagc atagctttcc tcgcagagtt ggcccatttc
     7561 cgcaaacgcc agaaaaatca gcgcgacggc cgcaaaaggg gagcgggaat tgcaggatgc
     7621 aatctctgta acaagcgtag cgggtttgct tacaccctcc ggttaaggaa tgtacaatca
     7681 aaccagttga cgcctgaatt gggcagttaa tgaccttttt accctaccag tgtttaaatt
     7741 tgtgatcaac ttcacacatg gatttcgttt aggactcatt gatgtaactg attatttacg
     7801 ccaaatttac agagaagatt tcctcgacca ttgcccccgc cagacgctgc gctgaacaga
     7861 gtcgtggcat ccccagcgcg acagtttgcc agcgttgggt cactgaccag caaacgggtt
     7921 gtttatctgc cgtggtccac tttcccagcc aacttaaaaa atcagcatga tccccgcccg
     7981 tcggaacggt cgaagaggca aaccactgat ggtaatagtg acgcgtggtc ggggccgcat
     8041 tgatactctc tgccagatgt tgtgtcgctt tcacgcgcaa atcacccagc aacaacgcca
     8101 ccgcttgcgg gcttcccaga cgacaggcat cgccaaatgc cccccaacgc aactcttcga
     8161 gaacgataaa acagcgccca ggcaacgacc aggcgttata cgccccagca cgccagcggg
     8221 taaaaatttg ctcgctctgt tcacccgcct gcaccagtag gcctcgttgc gttaatgcct
     8281 tttccttcca gcgacaacgg gctgaaaacc actcatacaa ggcggcaatt tgctggggga
     8341 gctccgggaa agcagagtgc tggcggtgat ccattttttg cagcagcgcc agatgctgcg
     8401 cgatctgcgt cagtgccaga tgaccgggat ccagcatcga cgccagtttt tccaccaatg
     8461 tcagacgcag gctaagcggc gcatttaaca tgccgccgtt cgtccatgtc tgaagctgca
     8521 cgcgcgcaag aagttcctgc atcagtcgtt cacgaaactg ctgctgatgg gcttgtggaa
     8581 ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg gggataaacc gactccagca
     8641 cgcgaccggg gccgtcaagt aacgtcttgg tcaatatcgt tctgccgtga aaagtgtttg
     8701 aatatcatcg cgtaacagct gggcgtcggt gtaaatccag ccgtgagtca tcacagtctg
     8761 ctgcaattgc tgctgcatca gcctgaccac cgattcattt tgttgacgca gagccagact
     8821 ttcgcgtaaa cgcgtctgta attccgtcaa acatgaagcg aactcagcga aaaaagtatt
     8881 catgcctgcc gtaacagatt catcgacctg ctctgccaga actttagcca tttgttggca
     8941 ataaagatcg acttctgcgc ttaaagctcg ttgcaacaca ctgtaatcaa ccgtttctgt
     9001 cggggatttc tcatttcccc gtccccagtc gggctgattc aaccagcgcg aaaaagtctc
     9061 acgcacaacg cctaaacgcg tgctctgctc gtccgttgct tcctggcgcg aaatgacttc
     9121 actgaacagc tggcgagtgt tgaagtgggg aactacgccg tgaaaaacag gaaaatgaaa
     9181 cccaggacga aaccctgact cgctcaattc cattttgact tgttgctcaa tgggccgaat
     9241 aacatcggtt aacacccggc aaagggtgga ttcaagctcg gcaaaacgca gcgtaaagtc
     9301 gcgactgatg gtgttctgcg ccgtctgtaa cagtgtctca cagcaggtac gaatctcgct
     9361 taacggctcc gaatcatcct gaaacaaggc ggctaactgc gcattcagcg catcttgctg
     9421 ttgacgcaga aagtggttgg cggaggtcag ggccagctcg atttcatgtt taacctcgcc
     9481 gctcacctgc gcctgattga gttgcaatag ctgcaaactt tcttcgacct gatggatatt
     9541 ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca cgaaaatcca ggtatccccg
     9601 cgcctgctgc gcgtaattca acagtttatg cgcagcagat cgcagagcat acaacgaggc
     9661 gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg gcgaacagcg aatcttccca
     9721 caactgatcg gcagcatgac gaatatgttc gaggtccgcc agatcgtcat gacgccagcg
     9781 cctgccgagc gcagcatggg caaaatcttc cacccagcgt tgttgctctg gcgctggtaa
     9841 cttaccgttg ttggctaact catggcgcgc tcgattcgcc aggtagcccc acatcgacga
     9901 caccggaaat atctgctgtg gcgtaataca gcctttcatc agcgtcccgg aaatcagtgc
     9961 ccgcacctgg tcggcgtcgt cactgttacg atcctgttga tcgaacttat tgaccagcac
    10021 atacagcggc accgattgcc ccaccgccaa aatcgcctca cggacctctt catcggagat
    10081 cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc gaggcgcgtg ccagctgctg
    10141 gttaagcatt ttttgcagat gcggttgccc ggcttcattt ggcccggggg tatccagtaa
    10201 cgtcaactga ccgggataac tctccagccc cgccagatgg acaaactcca cttcaatcac
    10261 gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa tcgacgtcca gcgccttcgc
    10321 cagtcgcact aaatcattca aacttttcag acaatgaaaa ataggctggg cacccagata
    10381 atatttttcg aaagcgacgc cattttcgat ccgctgcata agcgcacgca tatctttatc
    10441 tatttccagc acatcggtca gatgcttaat atcgcaatca cgcaggcgct gttgtaattt
    10501 ttgaattaaa cggtcgattg gcgcgacatg tgaaaaatgc agtaccggtt ctttttgccc
    10561 gggcgtatgg cgaataagcg tcggcagcgc agtcattggg cgattacgat taggcaaaac
    10621 ctccgtacca acaatggcat taatggtggt tgatttccct gctttcatgg taccgacaat
    10681 cgccagcacc atttccagtc gggaaatttt acgcaactca ttattcagca tcgcgtgacg
    10741 ttcggcgata ttaggctgac tccagggtaa agccagttgt ggcgcatcgt ctccgggtac
    10801 agagagaggc attttttcca gtaactgcaa ctgttggcga gaaagctgta acaggcgttc
    10861 agcctcctga cttaactcat acagggtctg tgtgtacata gaaaattctt ccttaaagca
    10921 aattttgtta cttttattta gccagattgt ttttgagttc tgttttcggc ttttataatt
    10981 actgcaagaa ataattttat atttagcgtg ttgtttttta ttagaataaa taacgtcttc
    11041 cgatacgttt aaaacgtcag aaacataaaa atatcatgtg aattaaaaaa aagaacaagt
    11101 agagcattaa cattatctta aataataaat ggaggcaaaa agattatttt ctgtttgcgt
    11161 ttcctttcaa atgaaaacga tcgtcgtcta aaatcagcag tacccccgac aaactcaggg
    11221 attttgtgta taattgcggc ctttttcggc aatctgccgt tttttggcgc ttttgccctg
    11281 ctgacttttg aggaaatcca catgtcatta ccacactgcc caaaatgcaa ctccgaatac
    11341 acttacgaag ataacggcat gtacatctgc ccggaatgtg cctacgaatg gaacgacgca
    11401 gaacctgcac aggaaagcga cgagctgatc gttaaagatg ctaacggcaa tctgctggct
    11461 gacggcgaca gcgttaccat cattaaagat ctgaaggtga aaggtagctc ttcgatgctg
    11521 aaaattggca ccaaagtgaa aaacatccgc ctggttgaag gcgaccataa catcgattgc
    11581 aaaatcgacg gttttggtcc gatgaaactg aaatctgagt ttgtgaaaaa gaactgattg
    11641 tattgtgatc ggtaagccgg ataaggcgct cgcgccgcat ccggcaacga tgccgactgc
    11701 ctgatgcgac gctgaccgcg tcttatcagg cttctctctc atctgtataa atttcgaact
    11761 acacttaact ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    11821 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    11881 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    11941 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaacgtgcg cattgccgga
    12001 agcgacatca tgatgagcga tgctataccg tcaggaaaag ccagctactc cggctttacg
    12061 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    12121 aacggaaaaa tcgaaatggc ctggcaggaa actttctggg cgcatggctt tggcaaagtc
    12181 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    12241 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    12301 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    12361 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    12421 catcaggcgc tgcatgcggt tgatctgaac attcatcacg gtgaaatggt ggctctgctt
    12481 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    12541 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    12601 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    12661 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    12721 ttctggcgca cctgttttag ctggttcacc ggcgagcaga aacagcgcgc gttacaggcg
    12781 ctgacccgcg ttggcatggt gcattttgcc catcagcgcg tttccaccct ctccggcgga
    12841 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    12901 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    12961 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    13021 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    13081 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    13141 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    13201 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    13261 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    13321 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    13381 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    13441 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    13501 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    13561 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    13621 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    13681 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgctgg gcatgaaacc
    13741 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    13801 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    13861 ccgctgatcc caggcgatcc gatcgtctgg cgtaaaaacc tttccgaaac caccaaagac
    13921 aagatctacg acttctttat gaactacggc aaaacgccgg aagaaaaagc ggtgctggaa
    13981 cgcctgggct gggcgccgtt ccgcgcttcc agcgacctgc aactggtgcc gattcgccag
    14041 ctcgcgctgt ttaaagagat gcagggcgtg aaaagcaata aaggactgaa tgagcaggac
    14101 aagctggcaa aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    14161 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aaatcgtagg tcggataaga
    14221 cgccccggcg tcgcatccga caatgtgcag gcgttgatgc cggatgcggt gcaagcacct
    14281 tatccggcct acagaccgga gccaaacatg caaaccatca ccatcgcccc acccaagcgc
    14341 agctggttct cgcttctgag ctgggccgtt gttctcgccg tgttggtcgt ctcgtggcag
    14401 ggcgcggaaa tggccccgct cacgctgatt aaagacggcg gcaacatggc gacgttcgcc
    14461 gccgacttct tcccgcccga tttcagccag tggcaggatt acctcaccga aatggccgtc
    14521 acgctgcaaa tcgccgtctg gggcacggcg ctggcggtgg ttctctccat ccccttcggc
    14581 ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc agcccgttcg ccgcctgatg
    14641 gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc tgttcgtggt cgccgtcggc
    14701 ctcggcccgt tcgctggcgt gctggcgctg tttatccaca ccaccggcgt gctctccaaa
    14761 ctgctttccg aagcggtgga agcgattgaa cccggcccgg tggaaggcat tcgcgccacc
    14821 ggcgccaaca agctcgaaga gatcctctac ggcgtgctgc cgcaggtgat gccgctgctg
    14881 atctcctact ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt cgtcggcatg
    14941 gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt ccagttccaa
    15001 caaacctgcg ccctgatggt gcttatcatc gtcacggtca gcctgctgga tttcctctct
    15061 caacggttgc gtaagcactt tatctgataa gcgaggcatt gatatctatg cacttgtcta
    15121 cacatccgac cagctaccca acacgctatc aggagatagc cgcaaaactt gagcaggagc
    15181 ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga gcagcaactg gcggcgcgct
    15241 ttgaggtgaa ccgccacacc ctgcgccgcg ccatcgacca actggtggaa aaaggctggg
    15301 tacagcgccg tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat tacccgctca
    15361 acgcccaggc gcgttttagc cagaacctgc tggaccaggg tagccatccc accagcgaaa
    15421 aactgctgtc ggtattgcgc cccgcgtccg gtcacgtcgc tgacgcgctg gggattaccg
    15481 agggcgagaa cgttatccat ttgcgcaccc tgcgtcgcgt caacggcgtc gcgctctgtt
    15541 taatcgacca ctactttgcg gacctcaccc tttggccgac gctgcaacgc ttcgacagcg
    15601 gttcgctgca cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc agccagacgc
    15661 ggatcagcgc ccgtcgcgcc caggccaaag agtgccagcg tcttgaaatt ccgaatatgt
    15721 cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc ccggcggagt
    15781 actccgtcag cctgacgcgc gccgacatga ttgaattcac tatggagcac tgaatgcacg
    15841 cagataccgc gacccgccag cactggatgt ccgtactggc gcacagccaa ccggctgaac
    15901 tggcagcccg cctgaaagca ctgaacatca ccgccgacta tgaggttatc cgcgccgccg
    15961 aaaccggcct ggtgcagatt caggcgcgga tgggcggcac cggcgaacgc ttttttgccg
    16021 gcgacgccac gctcacccgc gccgccgtac gcctgactga cggcacgctc ggctacagct
    16081 gggtgctggg gcgcgacaaa cagcacgccg aacgctgcgc gctgattgac gcgctgatgc
    16141 agcaatctcg ctattttcaa aacttatcag aaacccttat tgccccgctg gacgctgacc
    16201 gtatggcacg cattgccgca cgccaggccg aagtgaacgc cagccgggtc gacttcttta
    16261 cgatggttcg cggagacaac gcatgaccct ggaaaccgct tttatgcttc ccgtgcagga
    16321 tgcccagcac agttttcgtc gcctgttaaa ggcgatgagc gagccgggcg tgattgtcgc
    16381 cctgcatcag ctcaaacgcg gctggcagcc gctgaatatc gccaccacca gcgtgctgct
    16441 gacgctggcc gataacgaca cgccggtgtg gcttgccacg ccattaagta acgatatcgt
    16501 caatcagagc ctgcgttttc ataccaacgc gccgctggtc agccagccgg aacaggcgac
    16561 cttcgcggtg acggatgagg cgatttccag cgaacagctc aacgcccttt ccaccggcac
    16621 cgccgttgcg ccggaagcgg gcgcgacgct gattttacag gtcgccagcc tgagcggcgg
    16681 acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa cgaatgatcg ctccgcagct
    16741 gccagagtgc attctgcacg aactcaccga gcgcccgcac ccgttcccgc tcggcatcga
    16801 cctaatcctg acctgcggcg agcgcctgct ggctatcccg cgaaccactc atgtggaggt
    16861 gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga tcgacgccgc ccacgccctg
    16921 caagagagcc gacgccgggg cgataccgat ttgcccgaac tgagcatcgc ccagattgaa
    16981 cagcagctta acctcgcggt ggatcgcgtg atgacagaag gcggcattgc cgaccgcgaa
    17041 ctggcggcgc tggcgctgaa acaggccagc ggcgataacg ttgaagcaat tttcctgctg
    17101 cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg agccgctcga caccacggag
    17161 atgcgtctcg aacggcgtat ttcagcggtt tataaagata ttcccggcgg ccagctgctt
    17221 ggcccaacct acgactacac ccatcgcctg ctcgatttca ctctgctggc aaacggcgaa
    17281 gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt tttcagcctg
    17341 ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg gagcacagcc ggatgacatc
    17401 acccgcacgc cgccggttta cccctgttca cgttcttccc gcctgcaaca attaatgcgc
    17461 ggggacgaag gctatttgct ggcgctggcc tactccaccc agcgtggtta cggacgcaat
    17521 cacccgttcg cgggcgagat ccgcagtggt tacatcgatg tgtcgattgt gccggaagag
    17581 ctgggatttg cggtaaacgt cggcgaacta ctgatgaccg agtgtgaaat ggtcaacggt
    17641 tttatcgacc cgccggatga gccgccgcac ttcacgcgcg gctacgggct ggtattcggc
    17701 atgagcgagc gcaaagcgat ggcaatggcg ctggtcgatc gtgcgttgca ggctccggaa
    17761 tacggcgagc acgcgacagg cccggcgcag gatgaagagt ttgtgctggc acatgccgac
    17821 aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccag
    17881 gccgaactgg agctactcaa acgtctgcaa caggagaaga accatggcta atctgagcgg
    17941 ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg ccatcttaaa
    18001 agcggtggcg atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgccata
    18061 cggctgggga accggcggca tacagctcac cgccagcgtg attggcgaaa gcgacgtgct
    18121 gaaggtgatt gaccagggtg cggatgacac caccaacgcc gtgtcgattc gcaacttctt
    18181 taagcgcgtg accggggtaa acaccactga acgtacggac gatgcgacgc ttatccagac
    18241 gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag atcattatct tccaggtgcc
    18301 aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg gagacccgca ctatgcacgc
    18361 gctggaggag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca
    18421 tatcgccacc acctatgctt atccggtgaa ggtgaacggg cgctacgtga tggacccgtc
    18481 gccgatcccg aaattcgata acccgaaaat ggacatgatg cccgccctgc agctgttcgg
    18541 cgcagggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga
    18601 tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc
    18661 gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg
    18721 ctccgatacc gattattgcc gccaacagag cgaggcgaaa agccaatgaa tcaaccgtta
    18781 ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct
    18841 tttgatttat ggccggggga agtgctgggc attgtcgggg agtccggctc cgggaagacc
    18901 acgctgctga aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcactatgag
    18961 agccgttcgc tgtacgcaat gagcgaggcc gaccgccgtc gcctgctgcg caccgaatgg
    19021 ggcgtagtgc atcagcatcc gctcgacggc ctgcgccgcc aggtgtcggc aggcggcaat
    19081 atcggcgagc ggctgatggc gaccggggca cgtcattacg gcgatattcg tgccaccgcg
    19141 cagaagtggc tggaagaggt ggaaattccc gccaaccgga tcgacgacct gccgaccacc
    19201 ttttccggcg gcatgcagca acgtttgcag atcgcccgca acctggtgac gcatccgaag
    19261 ctggtgttta tggacgagcc gaccggcgga ctggatgtgt cggtacaggc acgcctgctc
    19321 gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tagtgattgt cacccacgat
    19381 ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg gcaagtggtg
    19441 gagagtgggt taaccgaccg cgtgctcgac gacccgcacc atccgtatac acaactgctg
    19501 gtgtcatcgg ttttgcagaa ttgaatgcaa caccgtgcgc gctgatcccc tcaccccggc
    19561 cctctcccgg agggagaggg agaaaaccgg cgcaatattc aacaccgcgc acaatcggtc
    19621 ccctcgcccc ttcggggaga gggttagggt gaggggcaat gcgcgcaccg ctccccaata
    19681 tgcgaaccac gaggcgaaaa atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc
    19741 accagcaaaa cggcgtgcgc ctgcccgtct tacaacgcgc ctcgctcacc gtcaacgcag
    19801 gcgaatgcgt ggtgctccac ggtcactccg gcagcggcaa atcaaccctg ctgcgctcgc
    19861 tgtacgccaa ctatctaccc gacgaaggtc aaatccagat caaacacggt gacgagtggg
    19921 tagacctggt caccgcgcca gcgcgcaaag tggtggaaat ccgcaaaacc accgtcggct
    19981 gggtgagcca gtttctgcgc gtcatcccgc gtatctcagc actggaagtg gtgatgcagc
    20041 cgctgctcga tacaggcgtt ccgcgtgaag cctgcgccgc taaagccgcg cgtcttctca
    20101 cccgcctgaa cgtgccggaa cgcctgtggc acctggcacc atcgacattt tccggtggcg
    20161 aacagcagcg cgtcaacatc gcccgcggct ttatcgtcga ctaccccatt ctgctgctcg
    20221 acgaacctac cgcctcgctg gacgccaaaa acagcgccgc ggtggtggaa ctgattcacg
    20281 aagccaaagc ccgtggcgca gccatcgtcg gcatcttcca tgacgaagct gtacgtaatg
    20341 acgtcgccga ccgcctgcac ccaatgggag cctcttcatg attatcaata acgttaagct
    20401 ggtgctggaa aacgaggtgg tgcacggttc gctggaggtg caggatggcg aaatccgcgc
    20461 ctttgccgaa agccagagcc gcctgccaga agcgatggac ggcgaaggtg gctggctact
    20521 acccggcctg attgagctgc ataccgacaa cctcgataaa ttcttcaccc cgcgcccgaa
    20581 agtcgactgg cccgcccatt cggcgatgag cagccacgac gcgctgatgg tggcaagcgg
    20641 catcaccacc gtactggacg ccgtggcgat tggtgatgtg cgcgacggcg gcgatcggct
    20701 ggagaatctg gagaagatga tcaacgccat cgaagagacg caaaaacgcg gcgtcaaccg
    20761 cgccgagcac cgcctgcacc tgcgctgcga actgccgcat cacaccacac tgccgctgtt
    20821 tgaaaaactg gttcagcgcg aaccggtgac gctggtgtcg ctgatggacc actcaccggg
    20881 ccagcgccag ttcgctaacc gcgagaagta tcgcgaatat tatcagggca aatactccct
    20941 caccgatgcg cagatgcagc agtacgaaga agagcaattg gcgctcgccg cacgctggtc
    21001 gcagccgaat cgcgaatcca tcgccgccct gtgccgcgcg cgacaaattg cgctcgccag
    21061 ccacgatgac gccacgcacg cccacgttgc cgaatcccac caacttggca gcgtgatcgc
    21121 cgaatttccc accacgttcg aagcggcgga agcctcgcgt aagcatggca tgaacgtgct
    21181 gatgggtgcg ccaaatatcg tgcgtggcgg ctcgcactcc ggcaatgtgg cggccagtga
    21241 actggcgcag cttggtctgc tggatatcct ctcttccgac tactaccccg ccagcctgct
    21301 ggatgcggcg ttccgcgtcg ccgatgacga gagcaaccgc tttacgctac cgcaggcggt
    21361 gaggctggtg accaaaaatc cggcgcaggc gctgaatctt caggatcgcg gggtgattgg
    21421 cgagggtaaa cgcgctgacc tggtgctggc gcatcgccag ggcaatcaca ttcatatcga
    21481 ccacgtctgg cgtcagggta aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg
    21541 gggccgtccg gctccgggaa agacagcctg ctggcggaac tccgcctgcg ggaacaaacg
    21601 cagttactgg tggcgcatcg ctacatcacg cgcgacgcca gcgccggaag tgaaaaccat
    21661 atcgccctga gtgagcggga gttttttacc cgcgcggggc aaaacctgct ggccttaagc
    21721 tggcacgcta acggtctgta ttatggcgtc ggcatcgaga ttgacctctg gctgcacgct
    21781 ggattcgacg tagtcgtcaa cggttcacgc gcgcatttgc cgcaggcacg ggcgcgctat
    21841 caatcggcgc tgctgcccgt ctgtttacag gtttcgccgg agatcctgcg ccagcgcctg
    21901 gaaaaccgtg gccgtgaaaa cgccagtgaa attaacgccc gcctggcgcg cgccgcccgc
    21961 tatactccac aggattgcca tacgctcaac aatgacggca gcctgcgcca gtcggtcgac
    22021 acgctgctga cgctgatcca tcagaaggag aaacaccatg cctgcttgtg agcttcgccc
    22081 ggccacgcag tacgataccg acgcggttta cgcgctgatt tgtgagctaa aacaggcgga
    22141 gtttgaccac catgcgtttc gcgtgggctt taacgccaac ctgcgcgacc caaacatgcg
    22201 ctaccatctg gcgctgctgg atggcgaagt tgtcggcatg atcggcctgc atttgcagtt
    22261 tcatctgcat catgtcaact ggatcggcga aattcaagaa ctggtggtga tgccgcaggc
    22321 gcgcggtctg aacgtcggca gtaaattact ggcatgggca gaagaagaag cccgccaggc
    22381 cggggccgaa atgaccgagc tttccaccaa cgtgaagcgc cacaacgcac accgttttta
    22441 cctgcgtgaa ggctacgagc agagccactt ccgcttcacc aaggcgctgt aacatgagcc
    22501 tgacccttac gctcaccggc actggcggcg cacagggcgt tccggcatgg ggctgcgagt
    22561 gcgcggcctg cgccagagcg cggcgctcgc cgcagtatcg ccgccagcca tgcagcggcg
    22621 tagtgaagtt taacgacgcg atcaccctga tcgacgccgg gcggcacgat ctcaccgatc
    22681 gctggtcgcc tggatcgttc cagcagtttt tgctgacgca ctaccatatg gatcacgtcc
    22741 aggggctatt tccgctacgc tggggcgtgg gcgatgtgat cccggtctac ggcccgccgg
    22801 atgaacaggg ctgcgacgat ctgtttaaac atccgggcct gcttgatttc agccacacag
    22861 tggaaccgtt tgtggtgttt gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc
    22921 actcaaaact gaccttcggt tatctgctgg aaacggcaca tagccgggtg gcgtggctgt
    22981 ctgacaccgc tggcctgccg gaaaaaacgc tgaaattttt actcaataat catccgcagg
    23041 tgatggtgat tgattgcagt cacccgccgc gcgcggatgc accgcgtaat cactgtgatt
    23101 taaataccgt gcttgcgctg aatcaggtta tccgctcgcc acaggtgatt ctgacccata
    23161 tcagccacca gtttgatgcg tggctgatgg aaaacgcact accgtcaggg tttgaggtgg
    23221 ggtttgatgg gatggagatt ggggtggcgt gatgagaggg aatgtgcgcg ctggccccct
    23281 caccctaacc ctctccccag aggggcgagg ggaccgattg tgctcaatat tgaatattgc
    23341 gctcgttttc tccctctccc cattggggtg aggggcgatg cctgctccat acccaacctc
    23401 atcgcccata ctcatcttcc attctccgct cttcatcctc cagttgccga cgctcctgat
    23461 caagctggcg ctggcgatcg tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt
    23521 cgtcatattg tctgcgccga tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat
    23581 cttcatagtc tcgcccacgg tcagggttat aagcgtcatt aatcgcctgc tgaatattac
    23641 caatagtatc gtcgataata tcagcctggg ccggaacggt ggacagcgtg agcagggtga
    23701 ataaaagaaa tagcggaaag cgtttcatta gccaacctca aaaagaaact ctatccacat
    23761 tagtcattac tcatccatgc aagtagtgga tgaatctcaa tttctccgct gctctattgc
    23821 cgtaatcgcc tccacgcgtt gttgatgacg accgccttcg tactgtgcgc ccagccacgc
    23881 atccacaatc atttttgcca gttcgaggcc aaccactcgt gaaccaaaag ccagcacgtt
    23941 ggtgtcgtta tgctgccgcg aaagttgcgc ggaataaggt tcgctacaga cgaccgcgcg
    24001 aattccggca aacttgttcg ccgctatcga aataccgacg ccagtaccac aaatcaaaat
    24061 cccgccatca acctctccgc cagcaacagc cagtgcgact tgactggcgt aatgtggata
    24121 atcagtacgc tctgacgacc aggttccttt atcaatcact tcaacgccac gctcaactaa
    24181 atgtgccact atttcatgtt ttaaaatgaa accgacatga tcacagccaa atgcaatctt
    24241 tttcattcat cttccccgtc cgtgcaaaac ttcacaatca aaaaacattt gtgatgttaa
    24301 tgaattaaaa acacccaaaa tcgatgaatt acgccttcct gatctgtttt acagagttac
    24361 atctgacagc gcaatagcgt taaagacact caccatccag caacatctat catctaaaaa
    24421 accagaaaaa caaataacat catgttttta aactaattaa atgaaataaa att
//
LOCUS       DAPHJE010000067        23579 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPHJE010000067 DAPHJE010000000
VERSION     DAPHJE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.067"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1470
                     /gene="tnaA"
                     /locus_tag="RH222_002725"
     CDS             55..1470
                     /gene="tnaA"
                     /locus_tag="RH222_002725"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV3797718.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1561..2808
                     /gene="tnaB"
                     /locus_tag="RH222_002726"
     CDS             1561..2808
                     /gene="tnaB"
                     /locus_tag="RH222_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV3797719.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2940..4115
                     /gene="mdtL"
                     /locus_tag="RH222_002727"
     CDS             2940..4115
                     /gene="mdtL"
                     /locus_tag="RH222_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV3797720.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4090..5049
                     /gene="yidZ"
                     /locus_tag="RH222_002728"
     CDS             4090..5049
                     /gene="yidZ"
                     /locus_tag="RH222_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV3797721.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5206..5955
                     /locus_tag="RH222_002729"
     CDS             5206..5955
                     /locus_tag="RH222_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV3797722.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5977..6543
                     /gene="yieF"
                     /locus_tag="RH222_002730"
                     /gene_synonym="chrR"
     CDS             5977..6543
                     /gene="yieF"
                     /locus_tag="RH222_002730"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV3797723.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6597..7934)
                     /gene="adeP"
                     /locus_tag="RH222_002731"
     CDS             complement(6597..7934)
                     /gene="adeP"
                     /locus_tag="RH222_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV3797724.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8101..8766
                     /gene="yieH"
                     /locus_tag="RH222_002732"
     CDS             8101..8766
                     /gene="yieH"
                     /locus_tag="RH222_002732"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV3797725.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8833..9306
                     /locus_tag="RH222_002733"
     CDS             8833..9306
                     /locus_tag="RH222_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797726.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9355..9942
                     /locus_tag="RH222_002734"
     CDS             9355..9942
                     /locus_tag="RH222_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDV3797727.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10004..10726)
                     /locus_tag="RH222_002735"
     CDS             complement(10004..10726)
                     /locus_tag="RH222_002735"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV3797728.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10741..11910)
                     /locus_tag="RH222_002736"
     CDS             complement(10741..11910)
                     /locus_tag="RH222_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV3797729.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11937..13553)
                     /gene="bglH"
                     /locus_tag="RH222_002737"
     CDS             complement(11937..13553)
                     /gene="bglH"
                     /locus_tag="RH222_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDV3797730.1"
                     /translation="MFRRNLITSAVLLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13639..15033)
                     /gene="bglB"
                     /locus_tag="RH222_002738"
     CDS             complement(13639..15033)
                     /gene="bglB"
                     /locus_tag="RH222_002738"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDV3797731.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15052..16929)
                     /gene="bglF"
                     /locus_tag="RH222_002739"
     CDS             complement(15052..16929)
                     /gene="bglF"
                     /locus_tag="RH222_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDV3797732.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17062..17898)
                     /gene="bglG"
                     /locus_tag="RH222_002740"
     CDS             complement(17062..17898)
                     /gene="bglG"
                     /locus_tag="RH222_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDV3797733.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVHK
                     EH"
     gene            complement(18184..18909)
                     /gene="phoU"
                     /locus_tag="RH222_002741"
     CDS             complement(18184..18909)
                     /gene="phoU"
                     /locus_tag="RH222_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV3797734.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(18924..19697)
                     /gene="pstB"
                     /locus_tag="RH222_002742"
     CDS             complement(18924..19697)
                     /gene="pstB"
                     /locus_tag="RH222_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV3797735.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(19788..20678)
                     /gene="pstA"
                     /locus_tag="RH222_002743"
     CDS             complement(19788..20678)
                     /gene="pstA"
                     /locus_tag="RH222_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV3797736.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20678..21637)
                     /gene="pstC"
                     /locus_tag="RH222_002744"
     CDS             complement(20678..21637)
                     /gene="pstC"
                     /locus_tag="RH222_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV3797737.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21724..22764)
                     /gene="pstS"
                     /locus_tag="RH222_002745"
     CDS             complement(21724..22764)
                     /gene="pstS"
                     /locus_tag="RH222_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV3797738.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23012..>23579)
                     /locus_tag="RH222_002746"
     CDS             complement(23012..>23579)
                     /locus_tag="RH222_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3797739.1"
                     /translation="FAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQ
                     SCEINNLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSF
                     YATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFS
                     AAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         5919 a         5969 c         5628 g         6063 t
ORIGIN      
        1 cagatgtaat attcacaggg atcactgtaa ttaaaataaa tgaaggatta tgtaatggaa
       61 aactttaaac atctccctga gccgttccgc attcgtgtta ttgagccagt aaaacgtacc
      121 actcgcgctt atcgtgaaga ggcaattatt aaatccggta tgaacccatt cctgctggat
      181 agcgaagatg tgtttatcga tttactgacc gacagcggca ccggggcggt gacgcagagt
      241 atgcaggccg cgatgatgcg cggcgacgaa gcctacagcg gcagccgcag ctactatgcg
      301 ttagccgagt cagtgaaaaa tatctttggt tatcaataca ccattccgac tcaccagggc
      361 cgtggcgcag agcaaattta tattccggta ctgattaaaa aacgcgagca ggaaaaaggc
      421 ctggatcgca gcaaaatggt ggcattctct aactatttct ttgataccac gcagggccat
      481 agccagatta acggctgtac cgtgcgtaac gtctatatca aagaagcctt cgatactggc
      541 gtgcgttacg actttaaagg taactttgac cttgagggat tagaacgcgg tattgaagaa
      601 gttggcccga ataacgtgcc gtatatcgtt gcaaccatca ccagtaactc tgcaggtggt
      661 cagccggttt cactggcaaa cttaaaagcg atgtacagca tcgcgaagaa atacgatatt
      721 ccggtggtaa tggactccgc acgctttgct gaaaacgcct atttcatcaa gcagcgtgaa
      781 gcagaataca aagactggac catcgagcag atcacccgcg aaacctacaa atatgccgat
      841 atgctggcga tgtccgccaa gaaagatgcg atggtaccga tgggcggctt gctgtgcatg
      901 aaagacgaca gcttctttga tgtgtacacc gagtgcagaa ccctttgcgt ggtgcaggaa
      961 gggttcccga catatggcgg cctggaaggc ggcgcgatgg agcgtctggc ggtaggtctg
     1021 tatgacggca tgaatctcga ctggctggct tatcgtatcg cgcaggtaca gtatctggtc
     1081 gatggtctgg aagagattgg cgttgtctgc cagcaggcgg gcggtcacgc ggcattcgtt
     1141 gatgccggta aactgctgcc gcatatcccg gcagaccagt tcccggcaca ggcgctggcg
     1201 tgcgagctgt ataaagtcgc cggtatccgt gcggtagaaa ttggctcttt cctgttaggc
     1261 cgcgatccga aaaccggtaa acaactgcca tgcccggctg aactgctgcg tttaaccatt
     1321 ccgcgcgcaa catatactca aacacatatg gacttcatta ttgaagcctt taaacatgtg
     1381 aaagagaacg cggcgaatat taaaggatta acctttacct acgaaccaaa agtattgcgt
     1441 cacttcaccg caaaactgaa agaagtttaa ttaatactac agagtggcta taaggatgtt
     1501 agccactctc ttaccctaca tcctcaataa caaaaatagc cttcctctaa aggtggcatc
     1561 atgactgatc aagctgaaaa aaagcactct gcattttggg gtgttatggt tatagcaggt
     1621 acagtaattg gtggaggtat gtttgcttta cctgttgatc ttgccggtgc ctggtttttc
     1681 tggggtgcct ttatccttat cattgcctgg ttttcaatgc ttcattccgg gttattgtta
     1741 ttagaagcaa atttaaatta tcctgtcggc tccagtttta acaccatcac caaagattta
     1801 atcggtaaca cctggaacat tatcagcggt attaccgttg ccttcgttct ctatatcctc
     1861 acttatgcct atatctctgc taatggtgcg atcattagtg aaacgatatc aatgaatttg
     1921 ggctatcacg ctaatccacg tattgtcggg atctgcacag ccattttcgt tgccagcgta
     1981 ttgtggataa gttcgttagc cgccagtcgc attacctcat tgttcctcgg gctgaagatt
     2041 atctcctttg tgatcgtgtt tggttctttc ttcttccagg tcgattactc catcctgcgc
     2101 gatgccacca gcaccactgc gggaacgtct tacttcccgt atatctttat ggctttgccg
     2161 gtgtgtctgg cgtcatttgg tttccacggc aatattccca gcctgattat ttgctatgga
     2221 aaacgcaaag ataagttaat caaaagcgtg gtatttggtt cgctgctggc gctggtgatt
     2281 tatctcttct ggctctattg cacgatgggg aatattccgc gcgaaagctt taaggcgata
     2341 atctcctcag gcggcaacgt tgattcgctg gtgaaatcgt tcctcggcac caaacagcac
     2401 ggcattatcg agttttgcct gctggtgttc tctaacttag ctgttgccag ttcgttcttt
     2461 ggtgtcacgc tggggttgtt cgattatctg gcggacctgt ttaagattga taactcccac
     2521 ggcgggcgtt tcaaaaccgt gctgttaacc ttcctgccac ctgcgttgtt gtatctgatc
     2581 ttcccgaacg gctttattta cgggatcggc ggtgccgggc tgtgcgccac catctgggcg
     2641 gtcattattc ccgcagtgct tgcaatcaaa gctcgcaaga agtttcccaa tcagatgttc
     2701 acggtctggg gcggcaatct tattccggcg attgtcattc tctttggtat aaccgtgatt
     2761 ttgtgctggt tcggcaacgt ctttaacgtg ttacctaaat ttggctaaat ccttcaagaa
     2821 gccagccatt cgctggcttc ttgcctctca ggaaatcact tatgtccaaa tggcaactcg
     2881 cctgattctc cttcaccacg tatgctttgc gtcaccttac tatcaggacg ctttagccca
     2941 tgtcccgctt tttaatttgt agttttgccc tggttttact ttatcccgcc gggattgata
     3001 tgtacctcgt tggtttaccg cgcatcgccg ccgatctcaa tgccagcgaa gcgcagttgc
     3061 atattgcgtt ctccgtttat ctggcgggca tggcagctgc gatgttattt gccggtaaag
     3121 tggccgatcg ttcagggaga aagcctgtcg ccatccccgg cgcggcgcta tttattatcg
     3181 cttcggtgtt ctgttcactg gctgaaacca gcacgttatt tcttgcaggg cgctttctac
     3241 aggggttggg cgcaggctgt tgttacgtgg tggcgttcgc cattttgcgc gacacgctgg
     3301 atgatcgacg tcgggctaaa gtgctgtcgt tactcaacgg tattacctgc atcattccgg
     3361 tgttagcgcc ggtgctcgga catctgatta tgcttaaatt cccgtggcag agtctgttct
     3421 gggcgatggc aatgatgggc atcgcggtac tgatgttgtc tttgtttatt ttaaaagaaa
     3481 cgcgccctgc ggcccccgca gcttcggaca aaccacgaga aaatagcgag tcgctgctta
     3541 accgtttttt cctcagccgt gttgttatca ccaccctcag cgtttcggtg atcctcactt
     3601 tcgtcaacac gtcaccggta ttactgatgg aaatcatggg gtttgagcgc ggtgaatacg
     3661 ccaccattat ggcgctgacc gctggcgtca gcatgaccgt ttcattctcc acgccatttg
     3721 cgctgggaat ttttaagcca cgtacgttga tgatcacctc gcaggtgtta ttcctggcgg
     3781 cggggatcac ccttgccgtt tcaccttccc atgcggtttc tctgtttggt atcacactga
     3841 tttgcgccgg tttctcggtc ggttttggcg tagcgatgag tcaggcgtta gggccgtttt
     3901 cattacgcgc gggcgtagcc agctcgacct taggtattgc gcaggtttgc ggttcgtcac
     3961 tgtggatttg gctggcggcg gtggttggta tcggcgcatg gaatatgctg atcgggattc
     4021 tgattgcctg tagcatagtg agcctgttgc tgattatgtt cgtcgcgcct ggacgccccg
     4081 ttgccgctca tgaagaaatc catcaccacg cttgatctca atttgctgct ctgtctgcaa
     4141 ctgctgatgc aggagcgcag cgtgaccaaa gcggcgaagc ggatgaacgt aaccccttcg
     4201 gcggtgagta agtcgctggc aaagttaaga gcatggtttg acgacccgct ctttgtgaac
     4261 tcaccgctgg ggctgtcgcc cacaccgcta atggtcagca tggagcaaaa tctggcggag
     4321 tggatgcaaa tgagcaacct gctgctggat aaaccgcacc accaaacgcc gcgcggcctg
     4381 aagtttgagc tggcggcgga atcaccgctg atgatgatca tgcttaatgc gctgtcgaaa
     4441 cagatctacc aacgttaccc gcaggcgacc atcaaattac gtaactggga ttacgattcc
     4501 ttagatgcca ttactcgtgg tgaagtggat attggttttt ccggtcgcga aagccatcct
     4561 cgctcgcggg agctgttaag ctcgctaccg ttagccattg attatgaagt gctgtttagt
     4621 gatgtgccct gcgtctggtt acgcaaagat catccggcac tgcatgaaac gtggagtctg
     4681 gacacttttt tgcgttatcc gcatatcagc atttgctggg aacagagcga tacctgggcg
     4741 ctggacaatg tgttacagga gctggggcgc gaacgcacga ttgctatgag cctgccggaa
     4801 ttcgagcagt cactgtttat ggcagcgcag cccgacaatc tgctactggc gaccgcgccg
     4861 cgctactgtc agtactacaa tcaactccac caactgccgt tggttgcgct tcctctcccg
     4921 tttgacgaaa gccagcaaaa aaagctggaa gtccctttta ccctgctttg gcataaacgg
     4981 aacagccata atccgaagat cgtctggtta cgggaaacca ttaaaaatct ttacgcgtcg
     5041 atggcataac cgaatcgtat gaaacgcgac cctatttcac aataaaatgt aaaaattttg
     5101 taataaactt gtctgaatcg aacttttagc cgctttagtc tgtccatcat tccagtaaat
     5161 gattactctt gtattcataa tggaccatta agcatggagc gaaaaatggc gactcacttt
     5221 gcccgaggga ttttaacgga aggacatctg atttctgttc gtctcccctc ccagtgtcat
     5281 caagaagccc gaaacattcc ccttcatcgt caaagccgtt ttctggcgtc cagaggttta
     5341 ctcgcagaac tgatgttcat gctgtatggc attggcgaat tgccggaaat tgtcaccctg
     5401 ccgaaaggta aaccggtttt cagcgataaa aatttgcctt cgttttctat ttcctatgcc
     5461 gggaatatgg ttggcgtggc gttaacaacc gaaggtgaat gtggcctcga tatggaacta
     5521 cagcgtgcga cgcgcgggtt tcatagccca cacgcgcccg ataaccacac cttttcaagc
     5581 aatgaatcgc tatggatcag taaacaaaac gatcctaacg aagcgcgggc gcagctcatc
     5641 acgctgcgcc gaagcgtgct aaaactaacc ggtgatgttt tgaatgacga tccgcgcgat
     5701 ctgcagctgc tgcccattgc cggacgcctg aaatgtgctc atgtaaatca tgtagaagcg
     5761 ttatgcgacg cggaagacgt gctggtgtgg tccgtggcgg tcacgcccac gattgaaaag
     5821 ctcagtgtct gggagttaga tggcaaacac ggctggaaaa gcctgccgga tattcacagc
     5881 cgcgccaaca atcctaccag ccggatgatg cgttttgccc agctctctac cgtgaaggct
     5941 ttttcgccaa attgatagac aaccacagga gtcatcatgt ctgaaaaatt gaaggtggtt
     6001 acgttactgg ggagcctgcg caaaggctca tttaatggca tggttgcacg taccctgccg
     6061 aaaattgctc cggcgagcat ggaagtcaat gcgttacctt ccattgccga cattcccttg
     6121 tatgacgctg acgtacagca ggaagaaggt tttccagcaa cggtagaagc tctggcggaa
     6181 cagatccgtc aggctgacgg tgtggtgatt gtcacgccgg aatataacta ctcggttccg
     6241 ggtgggctga aaaatgccat cgactggctt tcccgcctgc cggatcaacc gctggccggt
     6301 aaaccggtgt tgattcagac cagctcaatg ggcgtgattg gcggcgcgcg ctgtcagtat
     6361 cacctgcgcc agattctggt tttcctcgat gcaatggtga tgaacaagcc ggaatttatg
     6421 ggcggcgtga ttcagaacaa agttgatccg caaaccggag aagtgattga tcagggtacg
     6481 ctggaccacc tgaccgggca attgaccgca tttggtgagt ttattcagcg agttaagatc
     6541 taaataaaaa acccgccagt aatcatgcat ggcgggtttt taatgcgcta tcgattttaa
     6601 tgagcgtcga taaatacaat cttcaggata aacatcagcg caacgatgat tacgcacggg
     6661 ctaaggtcac gcagacgccc ggtaccaatc ttcatcacgc agtaggagat aaagcccagc
     6721 gcaatacctt cggtaatcga aaagctgaac ggcatcatca cggcggtaat aaacgccgga
     6781 acagattcag taagatcctg ccagttcacg cgagcaagac ttgaggtcat caacacgcca
     6841 acgtagatca acgcgccagc tgcagcgtag cctggcacca ttcccgccag cggcgacaga
     6901 aagataacca gcaggaacag cagaccaaca accactgccg tcagaccggt acgaccgcca
     6961 accgatacgc cggaagagga ctcaatataa gccgtaacgg aagaagtacc gataaacgaa
     7021 ccggtcacgg aagagatact gtcgacatac agcgcctgct tcatgcgcgg gaatttaccc
     7081 ttctcatccg ccaggcctgc tttatcggtc acgccaatca gcgtaccgga ggagtcaaac
     7141 aggttgacca gcatgaaaga gaaaatcacc cctgccagcc cgaggttaaa cgacccggct
     7201 aaatctacat ggcccacgac agtcattacg ctcggcggcg cagaaacgat gccattgtag
     7261 tggacatcac ccagcatcca gcccagcagc gttgtcacca cgatggaaac cagcaccgcc
     7321 gcgtgaatgt tgcgcgaggc cagaatagcg atgatgaaga agccgaggat ccccagcagc
     7381 acgctgtgag aagtcaggtt accgatgctg accagcgttt ccgggttagc gacaatcaca
     7441 cctgcattct tcagtcccat cataccaatg aacagaccga taccgctggt gatgcccaca
     7501 cgcagactta ccggaatgtt agcaatcatc cagtaacgaa cgcggaaaat cgtcagtaac
     7561 agcagaccaa tcgcgcccca gaagattgcg cccatcccga cttgccacgg caagcccata
     7621 gcctgtacaa cgacaaaagc gaagaatgca ttcaggccca tagcgggtgc cagtgcaact
     7681 ggcaggttag caaacagtcc catcataata ctgccgaatg cagcaatcag acaggtagta
     7741 acgaagacgg cgctggtatc catgccagca acgccaagaa tttgcgggtt aacaaaaacg
     7801 atgtaaacca tcgtcaggaa ggtggtaaaa ccggcgatca cttcggtccg tgccgtcgtg
     7861 ccatgttcgc gcagtttaaa cacgcgttcc agcatcccct ggccagaagc ctgggttgtg
     7921 tgttgttgac tcattatcta tttccgaaca aggagggaaa atccgtcgct atcgtatacc
     7981 aaaatgagac aatatggacg gctgaagata actttttttc attgtatttg cttatacggc
     8041 aacgattgcg tcatgcgaat tggcatgacg aaaacgttaa actaacgata aaggaaagac
     8101 atgtcccaga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     8161 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     8221 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     8281 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     8341 cggctgttcg attcagaact ggaagccatc gaaggggctg gagcgctcct gtcagcgatc
     8401 actgcgccaa tgtgtgtggt atctaacggc ccaaataaca aaatgcagca ttctatgggc
     8461 aagctgaata tgttgcacta cttcccggat aaactgttca gcggctacga tattcagcgc
     8521 tggaagccag acccggcgtt aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa
     8581 aactgcattc tggttgatga ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg
     8641 gaagtgttct acttctgcgc cgacccgcac aataagccga tcgttcaccc gaaagtcacc
     8701 acctttaccc atctttcgca gttaccggaa ctgtggaaag cgcgtggttg ggatattacg
     8761 gcatagttct tcacactccc ttcacttacc ccgcttaaat tggcgctcaa aggtaagtaa
     8821 agggagtttg atatgtctgt ttcacgtcgg gtaatacatc acggacttta ttttgcggtt
     8881 ttaggaccgt taattggtgt tctgtttctt gtcctctaca tattcttcgc aaaagaaccg
     8941 ctggttcttt tgcgaagaat acaggtactt ccattgtttc tcttattgtc gattactacg
     9001 ggagttattc ctgcattgtt aaccggcgtg atggtcgcct gtctgccgga aaagatcggg
     9061 tcacagaaaa attatcgttg tctggctggt ggcataggtg gcgtcgttat caccgagatc
     9121 tattgtgcag ttattgtaca tattaagggc atggcttcct cggagttgta tgaaaacatt
     9181 ctttctggtg acagtctcgt tgtccgcatc attcctgcat tgctggcagg tgtggtgatg
     9241 agcagaatca ttacccgtct gcccggattg gatatttcat gtcctgaaac agactcttta
     9301 agttaagcgg gatactttat ctttgggcta ctcaaaagca gacaggatgt tactatgact
     9361 caaaatatca ggccgttacc ccaattcaaa tatcatccca agccactgga aacaggcgca
     9421 tttgaacagg ataaaaccgt agagtgcgat tgctgtgaac aacagacgtc agtttattac
     9481 tcgggtccct tttattgcgt tgatgaagtt gaacatctct gtccgtggtg tattgcggac
     9541 ggttctgctg ctgaaaagtt tgcaggtagt tttcaggatg atgccagcat agaaggtgtt
     9601 gaatttgagt atgatgaaga ggacgaattt gccggtatta agaacacata tcctgatgaa
     9661 atgctgaaag agttggttga acgcacgcca ggttatcatg gatggcaaca ggaattctgg
     9721 cttgcgcatt gtggcgattt ctgtgctttt atcggctatg tgggctggaa tgatataaaa
     9781 gatcgcctcg atgaatttgc caaccttgaa gaagattgtg agaatttcgg tattagaaat
     9841 tctgatctag ctaaatgcct gcaaaagcgt ggtgattgtc agggttatct cttccgctgt
     9901 ctccactgcg gcaagctgag actgtggggt gatttttcgt agttatttaa ataatgagaa
     9961 caggccggag cgtaattcat acatccggcc ttatttcttc agcttaaccg agcgctaatt
    10021 ctgcggcagc ggctttatca gcgattacca tcagcgaagg atgcagctgc aacacggaag
    10081 ctggaacatc ttcggtaacc ggaccttgca gcacattttt cagtgcctgc gctttacctg
    10141 cgccactgac aattatcagc aagtttttcg ccgccatgat gcttttcggc cccatcgtca
    10201 cgtagctgtc aggcactaac gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa
    10261 ccatctctcc ctggatcggg aactccacgg tttgctcatg gaaatgggtc gtattcggca
    10321 aattgccaca aaaatgacca tctgcaccta atcccagcac caccaaatcc aggccgcctt
    10381 cacgcgccag tttctgatca tgctcgcggt agttatcaat agtgagcttc tggatattct
    10441 cttctttgat ccccgcaggg gtgaaaaaca gattacgcag attggtaatc gttacacctt
    10501 ctccctcttt gccgcgaaag ggaatttcat cgaaattata gaaatagcag ttatcatacc
    10561 agggcttacc tttaaccagg gtggtgaggt attcatacat gcctttgggc gtgctaccgg
    10621 cggtaattgc caggttaaca cgacgcgtct tcgacatata accaagcaga tggtgtgccg
    10681 cgacacggct catttcctgg taatcttcgg taatgattaa tttcatctta gtgccttatt
    10741 ttatttaaat aatttctgta cgaaggctgc ataggcaggc cgccagacat ccatttcatg
    10801 gttcaggccg ggatattcct ggtaatcaaa cttaattttt ttctgctcaa gctcagtttt
    10861 cagcccggcg atatccttgc cggttacgac atctttatca ccaacgacca cagtaaaatt
    10921 acgtagttgc tggttaatag ctgccggatc gttcagccgg gccgcgacac cttcatccgg
    10981 tacggttgtc gtggtaacac cactgaatgt ggccagccag ccaaagcttt ccagatgatt
    11041 cattccggaa accagcgcct ggtacccgcc ttgtgaaagc cctgccagcg cgcgaccatc
    11101 ggcatcttta cggacattaa aacgcttgct aatcagcggg ataatatcgt tcatcagttc
    11161 gcgatctgcc gctttagcat tcagcggata aaagacttta cgtctttcct gaggcacgaa
    11221 atcttcggga ataatgccct tcgcatcggt ttcggtatcc gggatcacca ccagcatcgg
    11281 tttaattttc ccttcagcca gcaggttatc catgatttgc gggatacgcc cctgatcgat
    11341 agcggaacgt ccggtatcac caaaaccgtg atagaaatag agcactggca aaggctcgcc
    11401 catgccggtg tatcctggcg gggtccagac atacatctga cgttcagatt gcaatgcgtt
    11461 ggagtggtaa gttatggcga tcaaatcacc atgcgcaaca gaacgcgtat ccagataact
    11521 gcctggcacc agaatcatac tggagttaac ctggcgctga ggcttagtca ttgcggtgcc
    11581 tgtatcaatg ctgcgtacac catcaacatt gaaaaaatac tcgtacaggt tgcctttcag
    11641 gataggtgtg cgccacgacc agactcctgc ttcgtctttg gtcatcgggt gaatattgtc
    11701 cggaaccgga acacccacca ctacagagac attttttgcc ccaggggcaa agtagcgaaa
    11761 ggtcacgctg ttatcggcat tgacctgagt cacatactgt ttcaccggga tagtgggtgc
    11821 cgggctggct ggcatatccg ctgctatggc ccactgagca gaaataccgc tggcaatagc
    11881 cagcgttaaa gcagcaattt ttatattcat cgtaatattc ctttcattaa gttccattac
    11941 caccagattt cagcctgggc accgacagcg aactggtcgt ctttattgtc ttcgaaggtg
    12001 aagccatcca gttcgttttc cagggctttg atatacgtgg cgtagaaacg aatttccgga
    12061 cgcgaggtca acatactggt attgacttta aaggtatgga agagcgtggt tttataacca
    12121 gactcattaa atttattact attcgcatct ttgttttgct gggtgaaata gcccagttca
    12181 acacctgtct ggttatattg gtcccaaata taggccgggc gaacaaccgc acgaatagat
    12241 tcgaaatcag agtgtgcgcc tgtttcgtag ctataaatat cgttaccgaa ggagtaaaca
    12301 atcgcgttag ccacaataaa atgatcgccg atataggctt cgccctgtga agtcagacga
    12361 actgctgttc cgccggtgtg atcaccataa taacgaccat taaaggtggt aaatggactt
    12421 gcgccagcat aacggccaaa gttactggcg atagagttat tcgcaaccag gaaggagaat
    12481 tcgttgaagc cacctttatc aaatttctgc gttaaagatg tgccgaacat ccaggtatct
    12541 ttccagtcat aatacccgtt attatcctga ttatcttttt ccgatgcgct ttcgtttgcc
    12601 gtgacataac gaccactcac cattaaggtc gctttatccc ataacgggat atctttatag
    12661 cgtaaatcaa tagtattggt attaatctgc tgtttgtttt gcaggctgcg atcgtaatca
    12721 tcgacatctt cgcgaaccag cgcgatatca attttacccg gaccgacttt ccagttttcc
    12781 agacctacac ccgctgcggc atcagtacgc tgcgttttcc agtccagcat ctggatttca
    12841 attttcggcg caccgtgttt acccacccag aaatcagcct ctggcgcaaa gggcaggaaa
    12901 cctttggtgg taacgtacat atcggagaac tgcataaagt tctcgccacc ggcgttatcg
    12961 ccaaaccagc cggtagagta ctgctgacca acgttaccat ccatcatcac aacggcatca
    13021 acccgtttgc cgttttcgtt gtagacacgt tgttttaact gcaaatcaaa ccagccggag
    13081 tattcgttac caaagcggcc cagagaacca atcgcccatg atttaggtga accatgagag
    13141 gcggtccccc aaccagaacg gtaatagccg ttgtaactaa agccaatttc atctttcaca
    13201 aacttactga aatctttcag agtcatggaa gaataaatcg agcctgtctc actggcattt
    13261 ttctcttcat ttttgaccag tacagcatca ggttttgcgg cactactggt cgggaacgga
    13321 ttggcggcat acccttgatc attcgtactt acgctacgat tcaccgtcgc tggcgtatat
    13381 tttttcttct cttcatcttt atactttttg agttcgcttt gcgtttctct taacgctttt
    13441 tctaataact caaggcgttg ttccaccgtt aatgattcag ccaatgattg tgcagaaaag
    13501 gctaacggtg ccattaataa gacggcagag gtaataagat ttcgtctaaa cataatatcc
    13561 ctttatggtg caaagaaaga attaacgtat cgcatccaga ctattctgaa tgcgacgata
    13621 attaaggtgc tttattggtt atttttttaa tgacagcccc cgcgttttga tcacttctgc
    13681 ataccatccg aagcttttct tacgcgttct tgtgaggctt ccttcgccat tatcatcacg
    13741 atccacataa ataaagccgt agcgcttaga catttgtgaa tgagatgcac tgactaaatc
    13801 aattggcccc caactggtgt accccataat atccacacca tcggcaatcg cttcatttac
    13861 ctgtaccagg tgatcgttta aataggcaat tcgataatcg tcctgtatcg aaccatccgc
    13921 ttcaacgctg tcttttgcgc ctaatccgtt ctcgacaata aataacggtt tttgataacg
    13981 atcccaaagc gtatttaaca gaacccgtaa tccaaccgga tcaatttgcc acccccactc
    14041 tgaacttttc agatgcggat tggggatcat attcagtatg ttgccctgcg catttttatt
    14101 aatgctttcg tcgtgggaaa cacaaccagt catgtaataa ctaaaagaga tgaaatcgac
    14161 ggtatgtttt aaatcttctg cgtcactttc agtcatctca atggtgatat tgtggtcgcg
    14221 gaagaaacgc tgcatatagc cgggatactg gccacgcgcc tgaacatcac caaagaacat
    14281 ccagcgccgg ttctcttcca tggcctgcaa catatcctgt ggctggcagg tgagggggta
    14341 aaccagccca ccgagaagca tattgccgat tttcgcttcg gggagcaggc tatgacaggc
    14401 tttaactgcc cgcgcactgg caaccagttg atggtggata gcctgataaa cttccgcctc
    14461 gccactctct tctgccagcc ccacgcccgt gaatggcgcg tgtaacgaca tgttgatttc
    14521 attaaacgtc agccataacg ccactttatg ttggtagcga gtaaagaccg tgcgggcgta
    14581 atgctcgaag tgatcgatga ccgctcgatt agcccaaccg ccgtagtttt tcaccagccc
    14641 atatggcatt tcgtaatggg ataacgttac cagcggcttg atccccgcct gcgccatttc
    14701 atcaaacagc cgatcgtaaa acgctaaccc cgcttcattc ggttcgactt cgtcgccctg
    14761 agggaaaatt cgcgcccagg caatggaaat acgcagacag gtgaagccca tctcggcaaa
    14821 taacgcgata tcttccgggt aacggtgata aaaatcgatg gcgacatctt tgatattctc
    14881 tttccccagg atgcgcggtt ccatttttcc cattacgcca tgaggctgta aatctgaggt
    14941 cgagatccct ttgccatctt cctgccaggc accttccacc tgattggcag ctgttgcgcc
    15001 accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc gttagcgaat
    15061 gatggataac agcggttcac ctgcgcttat ctgcgccgtg ccgtggggta atacgtccgt
    15121 aaaatcatcg ctattactga ttaataccgg cgtcgtcaga tcaaatccgg cctcgcgaat
    15181 agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac ccacgttgac
    15241 gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg ataccgacat gaatcaggat
    15301 ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca acgaagcaat
    15361 tcgacccgca accggagaac gcacttcacc aaccgagggc agaatggcga tacctttacc
    15421 caacaggcca ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa tctctcccgt
    15481 catcggtgaa cagataccgc cctgctcagg tggtgtaata acctctggtg ttttctcttg
    15541 cggggcaccc tgcgctggct gacgtttagc ggtgatgaaa tgaagcatca ccgtaccgac
    15601 aaatgcgcaa ccgatggcaa tgacaccgcc aataacgctg gcccagacgg tgaaatcaat
    15661 tcccgttgac gggatggttt gcatgaaggt gaaaatactt ggcaaaccaa aggagtagac
    15721 tttcgtttgc gcgtagccaa tgatggtggc ccccaaagcc ccactgatac aggcgataac
    15781 aaaggggtac ttacgcggca ggttgacgcc atataccgct ggttcggtga taccaaacag
    15841 actcgtcaac gccgctgatc ccgccaccac ttttttctgc gcatcgcgtt cgcagaggaa
    15901 gacgccgagc gccgccccga cctgcgccat aatggcgggc attaacagcg ggatcatggt
    15961 gtcgtagccc agcacggtga agttattgat acacagcggc accaggcccc agtgcagtcc
    16021 gaacatgacg aagatttgcc agaagccgcc cattaccgcg cccgcaaatg caggaaccgc
    16081 ctgataaagc cagagataac cggcggcaat cagttcgctt atccaggttg atagcggccc
    16141 caccagcaga aaggtgacgg gtgtgataac catcagacat agcaatggtg tgaagaaatt
    16201 tttgattgcc gacggtaacc acgcattaag tcggcgttcc agaatgctgc acaaccaggc
    16261 agaaaaaata atgggaataa ccgatgacga gtaattcaac aatgtgaccg gaatacccag
    16321 gaaatccagc cccagcgcat ccgctttttg cccgttctcg aaagcagtca gaattaatgg
    16381 atgcactaac gctccaccaa tcaccatggc agtaaatgga ttaccgccga agcgtttccc
    16441 cgcggtgtat cccaggatta tcgggaagaa ccaaaacaag gcatcactgg cgctgaataa
    16501 aattaaataa gtaccacttt gttcggtcgt ccactgaaaa gtgagcgcca gagccagcat
    16561 acctttcagg atcccggttg ccgccatcaa accgatcaga ggcgtaaaaa tacctgaaat
    16621 aacataaaca aagcggttta gcagattacc tttatcatca ttttccggtg cctgttgcgc
    16681 tttttcgtca aggcctgcca cactgttaac cgccaggaag acatcggcca catggttacc
    16741 tatgaccacc tgaaactggc caccgctttc caccaccata ataataccgg gggtcttttt
    16801 cagtacctct gcttgcgctt tgctttcatc ctttaactta aaacgtaatc gcgttgcgca
    16861 atgcatcaga ctcacaatgt tatctgcgcc cccgactcct gcgactattt ttctggctaa
    16921 ctccgtcata acttgccctc taccgctttg cggcaaaact caaaaaaaaa acctgaaaaa
    16981 aacggcctga cgtgaatcaa gcaatttttt caggttttgc ccgcttagtg cggtaacaat
    17041 cctttactca gtaataatat ttcagtgttc tttgtgcacg cgctctatat ttatggctaa
    17101 aaacataatc tctgcgggtg agattttacg ttgatactgc aaaccaataa aaatggcgat
    17161 ccgctccgca cattgccatg cttgcgggta attttgtttt actgcttgtt gtaatgactc
    17221 atcactatcg ttaattgaag catgtccaag aatacgccag gataaaaact tcagatgcgt
    17281 aaccagtcgc tgataactca agctttcttc ctggtaatta aggctgaact gaaattttat
    17341 taattgcagc atttcgcgca ttaattgcgt gacacctgca acatcctcca tatttccgct
    17401 catttgggca ctgaccagat gcatggcaat aaagcccact tcatctttcg gtaactgcac
    17461 gcccaaccgt ttatcaataa tggttaacgc ttcttcccct agctggaact ctttcgggta
    17521 aagccgctgg atatcccaca gcagcgggtt gggcagtaac acgttttgct gaaagcgttt
    17581 aatcgcaaac tggcaatggt cagttagcga gatataaata ctgtcctgta attttcccaa
    17641 gcgctcctgc gctaaagaga taatacgatc acaggttgcc atcacctcaa gaggaatatg
    17701 acttaagagt tcgcttaatc gcccgttcag ttcatgactg ctcaaggcat actctttttc
    17761 tattccactt gagttaattc tttcgccagc gcgtttttga aagccaattc ctcgccccat
    17821 gacgactttt tcccgctgtt gatcatcaat aaccaccaca acattattgt tgagaatttt
    17881 ggtgatttgc atgttcatag caaggacctt ttttataaac aaaaaaaccc gacttcacca
    17941 gtattctctg gttatgtcag gttttgcctg cgaatgcagt aacaatccag tcatttatta
    18001 atggttttta taacgaacat ccaggttcgg aaattaattt aattgcgtgc agagaaaata
    18061 gcaatgcgct attgataaaa atatgaccat gctcgcagtt attaactttg tgtaatttta
    18121 ggaatttata aagttatata taacaaatcc caataattaa gttattggga tttgtctggt
    18181 gaattatttg tcgctatctt ttcccgccag cagtttatcc agctcatcac cgcctacatg
    18241 acggaaatcc tgccccttca cgtagtagaa gataaactca caaatattct ggcagcggtc
    18301 gccaatacgt tcgatagaac gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg
    18361 cgaatcttcc atcatgtagg tcatcagttg acgaacaata ccttcgtatt cctgatcgac
    18421 ttttttatct tcacgataaa tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc
    18481 cagcacgtcg tgcagcatct ggatggtatg acggcccagc gactccagac ttaccagcaa
    18541 cggctgatgc tgctgggaga atttctccag cgcagtacgg cagattttgt ccgccacgtc
    18601 gccaatacgc tccagctcgg caatggtttt actgatcacc ataaccagtc gcaggtcgct
    18661 cgccgtcggc tgacgtttgg cgataatgcg cacgcaggct tcatcgatcg ccacttccat
    18721 catgttgacg ttcttgtcgc cttcgatcac gcgcttcgcc agatcgctgt cctggttatg
    18781 catcgcggtg attgcatcag aaagctgctg ctccaccatg ccgcccatgg tcatcacctg
    18841 cgtgcggata ctttccagtt cggcgttgaa ctggccggaa atatgtttat taagattgag
    18901 actgtccata acgcactcct gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt
    18961 ttcttcgctg gcttggtgaa cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg
    19021 tacataaacg ccgtgtggtc ggaacaacgc gcagcctgct gcatgttgtg ggtgacgatc
    19081 accacggtgt aatcctgctt cagttcggtg atcagctctt caatacgccc ggtagagata
    19141 gggtcgagcg ccgaacacgg ttcgtcgagt agcagcactt ccgggcgaat ggcgatacca
    19201 cgcgcaatac acagacgctg ttgctgacca ccagagagag agtaaccgct ctggtgcaat
    19261 ttatctttgg tttcgttcca caatgcggct ttggtcaatg cccactgcac gcgctcgtcc
    19321 atgtcggcac gggagagctt ctcaaacaga cgaacgccaa aagcgatgtt gtcgtagatg
    19381 gacatcggaa acggcgtcgg tttctggaac accatgccca ctttcgcacg cagcagggcg
    19441 atatcctgag agttggtcag gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc
    19501 tgctccggat acagttcaaa cattttgttg aaggtacgca gcagcgtcga tttaccgcag
    19561 ccggacggcc cgataaacgc cgttacctgg tttttagcga tatccaggtt gatgtttttc
    19621 agggcatgga atttgccgta gtagaagttc aaattacgaa cctgaatttt actcggggca
    19681 gtttcaacca tactcattgc aatcttttcc tcatcgtacc tgatgctttt cgctaatcag
    19741 gcttaccgta ggccgggtaa ggcgttcacg ccgcatccgg caaagtgtta accgtgttta
    19801 ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca ggacgcacag ggtaatgatc
    19861 aataccccgg cccaggccag ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag
    19921 atcgtcaccg gcaggttggc gatcggctgc atcatgtccg tgctccagaa ctggttggag
    19981 agcgcggtaa acagcagcgg cgcggtttca ccggcaatac gggcaatcgc cagcaggata
    20041 ccggtcataa tcccggacac cgacgctttc agcgtaatcg cggagatcat cttccacttc
    20101 ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg gcaccagttt cagcatgttc
    20161 tcggtggtgc ggataacgat cggcacctgc aacagcgcca gcgcaattac gcccgcccag
    20221 ccggagaagt gctccatctg tgccaccacg atggtgtaga caaacagacc aaccacaatc
    20281 gacggcgcgg agagcagaat gtcgttaatg aagcgaatca cttctgccag ccaggattta
    20341 cgaccatatt ccgccagata aatccccgcc ataatgccca gcggcgtacc gaataccgtg
    20401 gcccacaaaa ttaacagccc gctacccgcc agggcgttcg ccagaccacc accttccgta
    20461 ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca taccgtcgat accgcgagtg
    20521 atggtggaca ttaaaatcca gatcagccag aacaggccga aggccatcgt cgccatcgag
    20581 agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct gcattttgcg gcgagattca
    20641 gccagcgccg cagtggtttg catttcaacc atagccatta gcgtgccccc tcattcttag
    20701 ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga
    20761 tcaggcccag ttccatcagt gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt
    20821 tcgccagcgc agaggtgata ctgttgcccg gcatatacag cgaggcgctg tcgagctggt
    20881 aggtgttacc gatgataaag gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca
    20941 gcatgatgcc gccgataaca ccatttttgg tgaacggaag aacgatacgc cagataactt
    21001 cccaggtggt gcagccaata ccgtaggccg actctttcat catcaccggg gtttgttcga
    21061 acacatcacg cattaccgcc gcaatgtacg gaataatcat gatggcgagg atcacgcctg
    21121 ccgcgaggat accgatacca aatgcggggc cagagaacag cgcgccaaca atcgggatat
    21181 tcgacatgat attgccgacc ggctcctgaa agtaaacggc gaacagcggc gcaaagataa
    21241 acaggcccca catgccgtaa acgatacttg gaatggctgc cagcagctca atggcgatac
    21301 ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac
    21361 tcaccgggac ggcgatcagc agcgcgataa acgaagtcac caacgtaccg tagatcggca
    21421 ccagcgcccc gtagatatcg ttcggtgcat cccactcttt ggtccatagg aaagccagac
    21481 caaatttctg aatgctcggc caggaggaga tgatcagaga gacaataatg ccacccaaca
    21541 tcaatagcac aatcagcgcc gccagtttta ccagcacgct gaaaattatg tcgccctttt
    21601 tacccggtgg gttaaaagca ggcttggttg cagccataag ttactcttca gttaaacgcg
    21661 tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac accgtacccg gcctggagtt
    21721 ttattagtac agcggcttac cgctactgtc tttaatattg gtcttccacg cagcgcgaac
    21781 ctgttcaact acactatccg gcaggctggc gtaatccagg tcgttcgcct gtttagcccc
    21841 ggttttgtac gcccagtcga agaatttcag cacttctgtg ccttgttctg gtttcttctg
    21901 atctttgtgg atcagaatga acgtggtaga ggtaataggc catgcatctt cgcctttctg
    21961 gttggtcaga tcctgagcga aggttttgct ccagtctgca ccttttgctg cattagcgaa
    22021 gttttcttcg gtcggactaa ccggtttacc atcagcggag atcagtttgg tgtacgccag
    22081 gttgttctgc ttcgcgtaag catattcaac ataaccaatt gcacccggca gacgctgaac
    22141 gaacgcggcg ataccgtcgt tacctttacc gcccagaccg atcggccatt ttacggtaga
    22201 gccagtacca acgttgtttt tccactcttc gttcactttc gccaggtagc tggtgaagac
    22261 gaaggaagtc ccggagccat ctgcgcggcg tactacagca atgttttgtg aaggcagttt
    22321 cagacccgga ttcagtttgg cgatggcttc atcatcccac ttcttgattt tgcccaggta
    22381 gatgtcgccg agggttttac catccagcac cagttcgcca gacttcagcc ctggaatgtt
    22441 aaccgccagc accacgccgc caatcacggt cgggaactgg aacagacctt cctgagccag
    22501 tttttcgtca gacagcggcg cgtcagaggc accaaaatca acggtattag cgataatctg
    22561 ttttacgcca ccggaagaac cgataccctg gtagttaact ttattaccgg tttctttctg
    22621 gtaagtgtca gcccatttgg catacaccgg cgcagggaag gttgcacctg cacctgtcag
    22681 gcttgcttct gcaaacacag agaaagcgct catcgataag gtcgcggcga caacagttgc
    22741 gacggtggta cgcataactt tcataatgtc tcctgggagg attcataaag cattgtttgt
    22801 tggctacgag aagcaaaata ggacaaacag gtgacagtta tatgtaagga atatgacatt
    22861 tttatgacag aaaggaatta ccaaataact tactgatttt aaatgtgaag atgaaaatcc
    22921 cacatgtcat ctttttgtca tgatgcattt aaaagtgata aagaaatggg tgagatgaca
    22981 acgatgtgat gaacaatcgt tgtcagacct attagcgcac aatctcaaca gttatcgtcg
    23041 ctgttgcagt aaattgcccc ggggctgggc gagctccagt aaggctcgca ggagcagctg
    23101 aaaaagttac actacctgat ttttcaccat acactgttga tttacccatg tctgttggca
    23161 actcaccacc gttcactttg atttccttat tatttttgtc gtaaattttt atccccacgt
    23221 tagagtttga agttacgacc attgaattgt ccgtattggg ttcggtagca taaaaagacg
    23281 tactgatatc atcggtattc agtatcccta ggttttcaca ctcaatagtg accgttttag
    23341 taattttatg tgttgttacc gcacttccaa caacagatga aaaatctgca gctggtattt
    23401 ctttaaaatc aaaataaact ggctgtaaat tatttatttc gcaactttgc ggagctgtaa
    23461 tatcgccccg aatataaaca tcagccatcg gctgtgagta atccaccggg gtgtcacggc
    23521 taattgttcc atacaatgtc actatctttg tttcaggaat aatcaatttt cctgcaaat
//
LOCUS       DAPHJE010000068        23559 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPHJE010000068 DAPHJE010000000
VERSION     DAPHJE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.068"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..1643
                     /locus_tag="RH222_002747"
     CDS             15..1643
                     /locus_tag="RH222_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV3797740.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1882..1941
                     /locus_tag="RH222_002748"
     CDS             1882..1941
                     /locus_tag="RH222_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3797741.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            2258..2317
                     /gene="ibsE"
                     /locus_tag="RH222_002749"
     CDS             2258..2317
                     /gene="ibsE"
                     /locus_tag="RH222_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV3797742.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2437..3870)
                     /gene="hldE"
                     /locus_tag="RH222_002750"
     CDS             complement(2437..3870)
                     /gene="hldE"
                     /locus_tag="RH222_002750"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV3797743.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWVNG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3918..6758)
                     /gene="glnE"
                     /locus_tag="RH222_002751"
     CDS             complement(3918..6758)
                     /gene="glnE"
                     /locus_tag="RH222_002751"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV3797744.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(6781..8082)
                     /gene="ygiF"
                     /locus_tag="RH222_002752"
     CDS             complement(6781..8082)
                     /gene="ygiF"
                     /locus_tag="RH222_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV3797745.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8324..8944
                     /locus_tag="RH222_002753"
     CDS             8324..8944
                     /locus_tag="RH222_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV3797746.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9008..10246
                     /gene="cca"
                     /locus_tag="RH222_002754"
     CDS             9008..10246
                     /gene="cca"
                     /locus_tag="RH222_002754"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV3797747.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(10336..11157)
                     /gene="bacA"
                     /locus_tag="RH222_002755"
     CDS             complement(10336..11157)
                     /gene="bacA"
                     /locus_tag="RH222_002755"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV3797748.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11247..11615)
                     /gene="folB"
                     /locus_tag="RH222_002756"
     CDS             complement(11247..11615)
                     /gene="folB"
                     /locus_tag="RH222_002756"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV3797749.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11720..12337
                     /gene="plsY"
                     /locus_tag="RH222_002757"
     CDS             11720..12337
                     /gene="plsY"
                     /locus_tag="RH222_002757"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV3797750.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12350..13282)
                     /gene="ttdR"
                     /locus_tag="RH222_002758"
                     /gene_synonym="dan"
     CDS             complement(12350..13282)
                     /gene="ttdR"
                     /locus_tag="RH222_002758"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV3797751.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13489..14400
                     /gene="ttdA"
                     /locus_tag="RH222_002759"
     CDS             13489..14400
                     /gene="ttdA"
                     /locus_tag="RH222_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV3797752.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            14397..15002
                     /gene="ttdB"
                     /locus_tag="RH222_002760"
     CDS             14397..15002
                     /gene="ttdB"
                     /locus_tag="RH222_002760"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV3797753.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15050..16513
                     /gene="ttdT"
                     /locus_tag="RH222_002761"
     CDS             15050..16513
                     /gene="ttdT"
                     /locus_tag="RH222_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV3797754.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(16556..17569)
                     /gene="tsaD"
                     /locus_tag="RH222_002762"
     CDS             complement(16556..17569)
                     /gene="tsaD"
                     /locus_tag="RH222_002762"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV3797755.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17807..18022
                     /gene="rpsU"
                     /locus_tag="RH222_002763"
     CDS             17807..18022
                     /gene="rpsU"
                     /locus_tag="RH222_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV3797756.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18133..19878
                     /gene="dnaG"
                     /locus_tag="RH222_002764"
     CDS             18133..19878
                     /gene="dnaG"
                     /locus_tag="RH222_002764"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV3797757.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20073..21914
                     /gene="rpoD"
                     /locus_tag="RH222_002765"
     CDS             20073..21914
                     /gene="rpoD"
                     /locus_tag="RH222_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV3797758.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(21993..22499)
                     /gene="mug"
                     /locus_tag="RH222_002766"
     CDS             complement(21993..22499)
                     /gene="mug"
                     /locus_tag="RH222_002766"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV3797759.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            22624..22699
                     /locus_tag="RH222_002767"
     tRNA            22624..22699
                     /locus_tag="RH222_002767"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22657..22659,aa:Ile,seq:cat)
     gene            complement(22753..23517)
                     /gene="nfeF"
                     /locus_tag="RH222_002768"
     CDS             complement(22753..23517)
                     /gene="nfeF"
                     /locus_tag="RH222_002768"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV3797760.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELVID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLEM
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         5672 a         6502 c         6051 g         5334 t
ORIGIN      
        1 ctgtgccctc ctggatgttt accgcgatta ttgccgtatg cattctgttt attattggaa
       61 ttattttcgc caggctctat cgtcgcgctt cggcagagca agcttttgtt cgtactggtt
      121 taggtgggca aaaagtggtg atgagcggtg gcgcaatcgt gatgccgatc tttcatgaaa
      181 taatccccat caatatgaat actctgaagc tggaagtcag ccgctcaacc attgatagcc
      241 taattacgaa agatcgtatg cgtgtcgatg tagtagtcgc tttctttgta cgggtaaaac
      301 cgtctgtaga aggcatcgcc actgcggcgc agacgttggg gcaacgcacg ctatcaccag
      361 aagacttacg tatgttggtt gaagataaat ttgtcgatgc cctccgtgca acagctgcac
      421 aaatgaccat gcatgagtta caggataccc gcgagaactt cgtgcaggga gtacaaaata
      481 ctgtcgcaga agacctgtcg aaaaacggtc ttgagctgga aagcgtttca cttaccaact
      541 ttaaccagac ctcgaaagaa catttcaatc cgaacaatgc ctttgacgcc gaaggtttaa
      601 ccaaactgac tcaggagacg gagcgccgtc gtcgcgaacg taacgaagtt gaacaggatg
      661 tagaagttgc ggtgcgtgag aaaaaccgtg atgcactttc gcgcaagttg gagattgaac
      721 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
      781 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      841 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      901 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacaacag caggttaccg
      961 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
     1021 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
     1081 ccactcgcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
     1141 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
     1201 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg
     1261 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
     1321 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
     1381 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatcgtg
     1441 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaacctg gcggaacaag
     1501 cattatcagc cgctctctct taccgcacac aggcaccgct gattgactcc ttgctcaatg
     1561 aaattggcgt ttcaggcggc tcactggcgg cattgacttc acccttaacc tcaacaactc
     1621 ccgtcgccga aaacgtagaa taaattttca gctatgaacg tccctggcac aaaaagccgg
     1681 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     1741 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     1801 tcatcaacaa tcgggaagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     1861 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttaatc
     1921 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     1981 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     2041 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     2101 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     2161 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     2221 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     2281 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     2341 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     2401 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     2461 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     2521 gccgttgacc cagacttctt tactcccggc gatctcttct ggtttatagt cgccgccctt
     2581 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     2641 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     2701 aacggggcgg gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     2761 cagacggtca cccagtttgc gagcatttgc cagataggag acgtgcccgg cgtgcaggat
     2821 gtcaaagaca ccgttggtca ttaccacctt ttcgccgcgt ttacgcgctg ccgctacagc
     2881 aagcttcagt tcctcttcgg tcatcacacc aaagccggta tctgcgcgcc cacgcactgc
     2941 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     3001 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     3061 caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt
     3121 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     3181 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     3241 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gcgttaacag
     3301 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacacc
     3361 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     3421 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     3481 cggatcaaca ccttcgaaac cttcttcaaa atccagacgg atcagctgtt ggttgcggga
     3541 aagtacccgt aatttggtaa tggtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     3601 gttgacgtcg gccagagatt tactcagcgc gcgcgctgca tcgtcaatgc ccgtcaaccc
     3661 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     3721 cggacgttct tcgatggtat tcactttaac cacgggcacc ggcgcttccg gcgagatacg
     3781 actggtgggg ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     3841 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
     3901 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccagt
     3961 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
     4021 tgatgaagtt catcgcgaag cgtagtgtaa gcacgggtca gcgccatcgc ttcctgctct
     4081 tccataatgt cgttttgcgc cagtagttcc agaatacgca cgttgtctga ccagcgcgtt
     4141 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
     4201 gtaattcccc cttcatcagc tttgatatca aagcgatcgc gatgtttatt gccgagatga
     4261 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttcacgcggc
     4321 agcgtcataa tctcgcgacg cactgcgtca aagtgcgcgg tgagctgcgg atcgccgtac
     4381 actacacgcg cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa
     4441 tcggcaaatg cttctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcga
     4501 gcatccactt catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt
     4561 tgcgccagac gcagataaaa ctgccgcccg tcgatttccc gctcaccgtc agtcatcgca
     4621 tccattgggc aatcatggag gaagataagg tcaagatcgg aactgtagcc taactcccag
     4681 ccgcccagct tgccgtagcc gaccaccgca aaaccacgcc cttcgcgttc gttcaggtga
     4741 ttcggcttac cgtagcgggc aaccatttga acccacgcct gctgaacgac ggcatctatc
     4801 atggcttccg ccagccaggt taagtgatcg ctcactttca tcaccggtag cgtaccggcg
     4861 atatccgctg cggcgatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
     4921 tgttgctctt cgtcatcttc cggcacgcgc agcaaatact ggcgcaactc atcgcggtag
     4981 gcatcggtcg ccgtcggctg gtaaagggtg tttggatcga gcaattcatc cagcaataat
     5041 ggataacgcg ccagctggct ggcaatcatc ggcgacgcgg cacacagaga aatcaaatgt
     5101 ttaagcgccg cggggaattc actgagcaat tctaaatagg tggtgcgggt aacaatcccc
     5161 accagcaagg cggtaatgcg cgacagcgta acggcagcgt cttcacgcgc acagacatca
     5221 cttagcagat gcggcatcag atggtcgagc acctgacgtc ctcgcggccc gatggtgcgc
     5281 ttatccagct ctttgcggaa atcggcaatc agcgttagca cctgtttgcg atcatcctcg
     5341 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
     5401 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
     5461 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcaa ttgcggccag
     5521 tcagcaaagt ccatcgccca cgccagccgc gcacgattaa gctcatcaga aggaagcgtc
     5581 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatac
     5641 gccactcgca attgttcagc atcgttttca gaaagcagat gcagctcggc aatggcgctg
     5701 agcgttggca gtaaagagcg cgattgcagc gacggttcgc gtccgccgcg aatgagctgg
     5761 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
     5821 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
     5881 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
     5941 ttagcataga cgccttcgct atcgcccata atccgcgcct tgaccatcgc gtaacgctcc
     6001 cagtcgcgcc cctgctcctg gtaataatct tccaacgcgg caaagctcag caccagcggg
     6061 ccactttcgc caaacggacg cagccgcata tccacgcgat agacgaagcc atcctgcgtt
     6121 ggttgatcca gcactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
     6181 agttcccggc gtccaccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
     6241 tcagaggaga aattcagttc cccgccgccc agcttaccca tgcctaaaat cagcagcggt
     6301 tgcgcttcgc cctgcgcatt gcacggcgtt ccccactcgc ggcagcaggc gtcatatagc
     6361 cagtcacgcg ccgcaacaat cagcgtctcc gccagatagc tgagctgctg caatatgctc
     6421 tcttcagtaa ccagtgccag cgtttgcgcc caggcgatac gtaccatgat gcgccgacgg
     6481 aacagccgca gctcgcgcat taacccggct tcgtcactca cattactgag cgcctcctgc
     6541 aaccatgcaa cgtaatgctg ccattcgtcg gcctgcggcg gttggctttc cagttccgtc
     6601 agccactctg gatgcgcact tatgctgtcc tgcacaaaat cactgaaagt aagtactgac
     6661 ttcgcctgtg cactaagtga ttcctcggct aaaggctctg gcagccgctc aacaacggtc
     6721 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
     6781 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
     6841 ttcaatttcg atgcgttgcc cggtcgcaat agcgtgatgc agcccctgcc agttctccag
     6901 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
     6961 acgcgtcagg cgtggcaact ggtcacggta gcgatcgcct aacggctggc agaaaacgct
     7021 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggagtcgct
     7081 gattttgccc tgcgctttgg catctaaaaa tggctgccat gctttgctta ccaaccattc
     7141 ggtcaacgcc agcttcgcca ttgccgtttc ggtagagtag accgccgtca cggcagacac
     7201 cgcagaagca atggtcgcct cgcattgagt cagcagatca cgtaagtgag tgctcgcttt
     7261 acgcggcaca ataccaccga acagcatcag cgtatgacgg accaggctaa tggctgccag
     7321 cacctgttct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat actgccattg
     7381 cgctaacgcc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
     7441 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
     7501 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
     7561 cagtttcagc accgcgcgcg tgtcgccgct aagcagttcc agttccagct cacagatagg
     7621 ttcagcaaat tcacccgctt tcacttcccc ctggtcgagg gcgatttcaa tttggctacc
     7681 atcgaccgcc accagccatt tttcgcgata aaaatcggtg ctgaacagcg gctgcacgcg
     7741 ggaggcgaga tcggcgggca attcgccgtt cggccagact tccgtcggta actgcgccag
     7801 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctgatgta agccgcctgt
     7861 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
     7921 gcccatatcg tgcccacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg
     7981 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
     8041 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
     8101 atgtcacata agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt
     8161 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
     8221 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
     8281 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
     8341 gatcggatta actttactcg cacttagcgc gactgccgtc tcacacgctg aagaaacgcg
     8401 ctatgtttcc gacgaactga atacctgggt ccgcagcggt ccgggagatc attatcgcct
     8461 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
     8521 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
     8581 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
     8641 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
     8701 agtggcgcag agcgacagcg tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa
     8761 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca
     8821 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
     8881 gctcggtctg gtactgccgc acctgatccc aagccgcaaa cgcaaagatc gctggatgaa
     8941 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
     9001 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt tagggctacc
     9061 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
     9121 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
     9181 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatgc
     9241 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
     9301 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
     9361 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg tattgcgcgt
     9421 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctggc
     9481 gttgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
     9541 gaaagagacg gaaagcgccc tcaccacccg caatccacag gtgttcttcc aggtactgcg
     9601 cgattgcggc gcactgcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
     9661 ccctgccaag tggcatccgg aaatcgatac gggtattcat accttaatga cgctctcaat
     9721 ggcggcgatg ctgagtccgc aggtcgatgt ccgtttcgca actttatgcc acgatctcgg
     9781 taaagggctg acgccgccag aactctggcc gcgtcatcat ggtcatggcc cggcgggtgt
     9841 taagttagtg gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
     9901 cagactggtg gctgagtttc acgatctcat ccacaccttc ccaatgctga acccaaaaac
     9961 catcgtcaaa ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact
    10021 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
    10081 gcaaggccgc tggttgcgcg aagcctggga agtggcgcag tcagtgccga caaaagccgt
    10141 cgttgaagcg ggatttaaag gtgtggagat tcgcgaggag ctgacccgac ggcggattgc
    10201 ggcggtagcc agctggaagg aacaacgttg cccaaagcct gaatgagtat tgggcatttt
    10261 gccggatgcg gcgtgaacgc cttattcggc ctacgcaatc gttgacaacg ccaagcatcc
    10321 gacactattc ctcaattaaa agaacacgac atacaccgca gccgccacaa taaagcgata
    10381 aatggcgaac gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat
    10441 cagcgccacc acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccgcttgt
    10501 caggaagccc cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggcaccgc
    10561 cagcaggaac gaaaactcgg aagcagcgta acggctcacc cccatcagca tcccacctga
    10621 aatggtcgcc ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat
    10681 cataaatgcc tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt
    10741 caggcattcg gcggcaatca gcaacaaacc gccaacgacc agcgcataca tcacatttat
    10801 cgggttaaac aatgacttaa tcgtgtcgtg gaacaacagc cccaatacca ccgccggaat
    10861 catccccagc aaaatgtgga tcagcgttaa acgacctttg ctttcacctt cgtgctgcaa
    10921 cgggcggcca aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac
    10981 cgccagaatt gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt cgccctcaaa
    11041 ccccaacaag tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt
    11101 caatccttcg accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa
    11161 taaaccccta aattattaaa atgtaccgct tgtccgaact actgcgtatg accaggttat
    11221 aaccgtttgg tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca
    11281 atgattacgc caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatacgc
    11341 acccacggcg agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc
    11401 accagcgcaa aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa
    11461 ctgaggcaat ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg
    11521 atatcgaaca ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg
    11581 attaccgaaa gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa
    11641 ccggatacca cttccggcga aatgtgcgta ttatccacag attaatcgtt gaacacgaat
    11701 tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct
    11761 ctgcggctcc atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg
    11821 aaccagcggc tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc
    11881 agccgtagca gtactgattt tcgacgttct gaaaggaatg ttgcccgtct ggggcgcgta
    11941 tgaattaggt gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca
    12001 catctggccc gttttcttcg gatttaaagg aggaaaaggc gttgctaccg cttttggtgc
    12061 catcgcaccc attggctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt
    12121 gctattgagc ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta
    12181 tgtctggtgg tttaagccac aattcacctt cccggtttcg atgctctctt gcctgatcct
    12241 gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac
    12301 gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct
    12361 gatagccttc atcgggcttg cccagccgtt gctggcacca tgccgccaga aattccacgc
    12421 agacacgtaa tttcatgctg cgatagagcg gctcccggta aacagcccag atattggcgc
    12481 tttgtgcata ctccggcaat acttgcacca gtttgccact ctccagaaac ggcaacacat
    12541 cccactcgga acgcagcata atccctttgc cctccagcgc ccattgcaac actatctcgc
    12601 cgctattgga ggaaagatgc ccgctaacct tcaccgattt tttctcctga ccgttcccca
    12661 actcccatat tccgtgggtc atatcgcgct ctttggtcac cagacagtca tgacgactta
    12721 attcctgtaa tgattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata
    12781 ttcttttatt tttcgttaac agatgcgcaa tataataatc aggaatttcg tcattaatac
    12841 gaatatccag atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat
    12901 gcacctgtaa ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc
    12961 gcccaaaacc aaaactacag ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg
    13021 tgacgtcatc gaccagtcgc tgatattggg tgaggatttc cagcgcatgt tcgtaacaac
    13081 gttggccact ttcagtcagc gccacgccgc gagcggagcg gttcagcagc gttgttgcca
    13141 gcgtattttc aaggatttgg atccgcttgg tgacaaatgc aggcgtctgc ccaagcgtcg
    13201 ccgctgcggc gctaaaactc ccgctgtgga cgatttctac cagtacctgc aagtctttgg
    13261 ctaaaggcca gctattcagc atcgttaatt atccgcagtt gtgataagcg cagtgtattt
    13321 cgcaaaacat tgccattccc tgctctggcg caatattcat tcttattttc gcgttgattc
    13381 acgaactgtt aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtggta
    13441 gtttgtggcg gtgaattttt ccagacaaat acaaaaactg gagttgccat gatgagcgaa
    13501 agtaataagc aacaggcagt gaataagttg acagagattg tcgctaactt taccgccatg
    13561 atttctaccc gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa
    13621 acgtcgtcga tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt
    13681 gacctgaatc gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt
    13741 tcccgcttcc cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggca
    13801 accgtcaaag cgccactacg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc
    13861 aaaaataccg gtagcggcgt accgtgggtc acctgggaca tcatccccga caatgacgat
    13921 gcggaaatcg aagtttacat ggcaggcggc ggctgcacgc tacctggccg ctcgaaagtg
    13981 ttaatgccgt cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa tatctccacc
    14041 ctcgccgtaa acgcctgtcc accggtactg gtgggcgtgg gcatcgccac ctcggtggaa
    14101 accgccgccg tactctcgcg taaagccatt ttgcgcccga ttggcagccg ccatcccaat
    14161 ccaaaagcgg cagaactgga gctacgcctg gaagaaggac tcaaccgtct ggggattggt
    14221 ccacaagggc tgaccggcaa cagttcagtg atgggcgtac atatcgaatc tgccgcccgc
    14281 catccgtcaa ccatcggcgt tgctgtctct accggctgct gggcgcatcg tcgcggcacg
    14341 ctgctggttc atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga
    14401 aaaagatcct gacaaccccg atcaaagctg aagatctgca agatattcgc gtcggcgatg
    14461 tgatctacct gaccggtacg ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg
    14521 aactgaaacg tccgatccct tacgatctca acggcaaagc gattttccac gctggcccca
    14581 tcgtgcgcaa aaacggcgac aaatgggaga tggtctccgt cggcccgaca accagtatgc
    14641 gtatggaaag ttttgaacgt gaatttattg agcagaccgg cgtgaaactg gtggttggca
    14701 aaggtggtat ggggccgctg accgaagaag gctgccagaa attcaaggcg ctacatgtga
    14761 ttttcccggc aggctgcgcg gtgctggcgg caacccaggt ggaagagatt gaagaagtgc
    14821 actggacaga gctcggaatg ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc
    14881 cgctgattgt ctctattgat acccacggca acaacctgat agccgaaaac aaaaagctgt
    14941 tcgccgaacg ccgcgatccc atcgtggaag agatctgcga gcacgtccat tacatcaaat
    15001 aaccctcccg gagaggctca cccctctcct ttttcgcagg cataacacga tgaaaccttc
    15061 cactgaatgg tggcgatatc ttgcgccgct ggcggtcatc gccattattg ctctacttcc
    15121 ggttcccgca gggctggaga accatacctg gctctacttt gccgttttta ctggcgtgat
    15181 cgttggactg atcctcgaac ccgtgccggg tgccgtggtg gcgatggtgg gtatatccat
    15241 catcgccata ctctctccct ggctgctgtt cagcccggag cagctcgctc agccaggctt
    15301 taaattcact gcaaaatccc tctcgtgggc cgtttccggt ttttctaatt cggttatctg
    15361 gctgattttc gccgccttta tgtttggcac aggctatgaa aaaaccgggc ttggacgccg
    15421 cattgcgctg attctggtga aaaagatggg acatcgcacg ctgtttctcg gctatgcggt
    15481 gatgttctcc gagctgatcc tcgcacctgt aacaccgtcc aactcggcgc gtggtgcggg
    15541 gattatctat cccatcatcc gtaacctgcc accgctctat caatcacaac caaacgacag
    15601 cagttcgcgc agcattggct cgtacatcat gtggatgggg attgttgccg actgcgtgac
    15661 cagcgccatt ttcttgacgg cgatggcacc aaacttgctg ttaattggac tgatgaaaag
    15721 cgcatctcac gccacgctga gttggggcga ctggttcctc gggatgttgc cgctcagcat
    15781 tttactggtt ctgctggttc cctggctggc ttacgtgctg tacccgccgg tactgaagtc
    15841 tggcgatcag gtgccgcgct gggcagagac ggaactgcag gcaatgggcc cgctctgttc
    15901 gcgtgaaaaa cggatgctgg ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg
    15961 tgattatatc gatgccgcga tggtcggtta cagcgtggtg gcactgatgc tgcttctgcg
    16021 cattatcagt tgggacgaca ttgtcagtaa taaagcggcg tggaacgttt tcttctggct
    16081 ggcctcgctt atcaccctcg ccaccggact caacaacacc ggttttatta gctggtttgg
    16141 caaactgtta gcaggcagct taagcggtta ttcgccaacg atggtgatgg tggcgttgat
    16201 tgtggtgttt tatctactgc gctacttttt cgccagcgcc acggcgtata cctccgctct
    16261 cgcaccgatg atgattgccg ctgcgctggc gatgccggaa atcccgctgc cggtgttctg
    16321 cctgatggtt ggtgcggcaa ttggtctggg gagcattctt acaccatacg ccaccggccc
    16381 cagtccgatt tactacggca gtggttatct gccaacggcg gattactggc gactgggggc
    16441 gatttttggg ctgatattcc tcgtattgct ggtgattacc ggcttactgt ggatgcccgt
    16501 ggtgttgctt taagtgagga cggtgccgga aattgctctc cggcacccaa tagttttacg
    16561 cagccggtaa ctccgccagc ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg
    16621 cgcctgcttt aaaccgcacc attccggcat aggcgatcat cgcgccgtta tcagtacaaa
    16681 attccggacg cgcgtagaac acttcgccgc ggcgtttttt catcatttca gccagcttcg
    16741 cccgtaacgt acggttagca ctcacgccgc ccgccatgac cagtcgctta aagcccgtct
    16801 gatccagcgc ccgcttgcac ttaatcatca gcgtatcgac caccgcatct tcaaaggcgc
    16861 gggcgatatc agcacgcgtc tggtcgtcgg tgccgttgtc acgaatggta tttgccgcga
    16921 aggttttcag gccggagaag ctgaaatcca gccccggacg gtcggtcatc ggacgcggga
    16981 agacaaagcg cccggcagta ccctgagccg ccattttcga cagtaacggc ccgccaggat
    17041 aatccagccc cagcagcttc gcggttttat caaacgcttc cccggcggca tcatcgatag
    17101 actcgccgag cagctcgtac tgaccaatgc cagtcacgct gattaactgc gtatgaccgc
    17161 cggaaacaag cagcgcaaca aacggaaatt ccggcgggtt atcttccagc atcggcgcta
    17221 acagatgccc ttccatatgg tgtacaggga tcgccggaac gtcccaggca aacgccagag
    17281 aacgccccac ggtcgcgcca accagtagcg cgccgactaa tccagggcct gcggtatagg
    17341 ccacagcatc aatgtctttt gccgttaaac cagactcctt tagcgccgcc tggatcaacg
    17401 gtacggtttt acgcacatga tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag
    17461 cgtgcaattt cacctgacta tacaattggt tggctaacaa acctttttca tcgtcgtaaa
    17521 tggcgatgcc ggtttcatcg caggaagttt caatacccag tacacgcatg acttgtttta
    17581 cctcgcttta ttaccgcgca gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc
    17641 cctggagaaa gcctcgtgta tactcctcac ccttataaaa gtccctttca aaaaaggccg
    17701 cggtgcttta caaagcagca gcaattgcag taaaattccg caccattttg aaataagctg
    17761 gcgttgatgc cagcggcaaa ccgaattaat caaaggtgag aggcacatgc cggtaattaa
    17821 agtacgtgaa aacgagccgt tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa
    17881 agcaggtgtt ctggcggaag ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg
    17941 taagcgcgct aaagcttctg cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc
    18001 acgccgcact cgtctgtact aattccccga gagcgttgct ctccgatcag accgagttgt
    18061 agttgtaagg ccgtgcttcc gaaaggaatg cgcggcttat tttcgtttat gaattgctaa
    18121 aaatcggggc ctatggctgg acgaatccca cgcgtattca ttaatgatct gctggcacgc
    18181 actgacatcg tcgatctgat cgatgcccgt gtgaagctga aaaagcaggg caagaatttc
    18241 cacgcgtgtt gtccattcca caacgagaaa accccgtcct tcaccgttaa cggtgagaaa
    18301 cagttttacc actgctttgg atgtggcgcg cacggcaacg cgatcgactt cctgatgaac
    18361 tacgacaagc tcgagttcgt cgaaacggtc gaagagctgg cagcaatgca caatcttgaa
    18421 gtgccatttg aagcaggcag cggccccagc cagatcgagc gccatcagag gcaaacgctt
    18481 tatcagttga tggacggtct gaatacgttt taccaacaat ctttacaaca acctgttgcc
    18541 acgtctgcgc gccagtatct ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt
    18601 gcgattggtt ttgcgccccc cggctgggac aacgtcctga agcggtttgg cggcaatcca
    18661 gaaaatcgcc agtcattgat tgatgcgggg atgttggtca ctaacgatca gggacgcagt
    18721 tacgatcgtt tccgcgagcg ggtgatgttc cccattcgcg ataaacgcgg tcgggtgatt
    18781 ggttttggcg ggcgcgtgct gggcaacgat acccccaaat acctgaactc gccggaaaca
    18841 gacattttcc ataaaggccg ccagctttac ggtctttatg aagcgcagca ggataacgct
    18901 gaacccaatc gtctgcttgt ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac
    18961 ggcattaatt acgccgttgc gtcgttaggt acgtcaacca ccgccgatca catacaactg
    19021 ttgttccgcg cgaccaacaa tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat
    19081 gccgcctggc gagcgctgga aacggcgctg ccttacatga cagacggccg tcagctacgc
    19141 tttatgtttt tgcctgatgg cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa
    19201 gcgtttgaag cgcggatgga gcaggcgatg ccactctccg catttctgtt taacagtctg
    19261 atgccgcaag ttgatctgag tacccctgac gggcgcgcac gtttgagtac gctggcacta
    19321 ccattgatat cgcaagtgcc gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc
    19381 aacaaattag gcatacttga tgacagccag cttgaacgat taatgccaaa agcggcagag
    19441 agcggcgttt ctcgccctgt tccgcagcta aaacgcacga ccatgcgtat acttataggg
    19501 ttgctggtgc aaaatccaga attagcgacg ttggtcccgc cgcttgagaa tctggatgaa
    19561 aataagctcc ctggacttgg cttattcaga gaactggtca acacttgtct ctcccagcca
    19621 ggtctgacca ccgggcaact tttagagcac tatcgtggta caaataatgc tgccaccctt
    19681 gaaaaactgt cgatgtggga cgatatagca gataagaata ttgctgagca aaccttcacc
    19741 gactcactca accatatgtt tgattcgctg cttgaactgc gccaggaaga gttaatcgct
    19801 cgtgagcgca cgcatggttt aagcaacgaa gaacgcctgg agctctggac attaaaccag
    19861 gagctggcga aaaagtgatt taacggctta agtgccgaag agcgatcggg aagcccccga
    19921 cagccgcact gagaggcagc ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg
    19981 taaacaaccg ttggatttca gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac
    20041 caacctcatg aaataagtgt ggataccgtc ttatggagca aaacccgcag tcacagctga
    20101 aacttcttgt cacccgtggt aaggagcaag gctatctgac ctatgccgag gtcaatgacc
    20161 atctgccgga agatatcgtc gattcagatc agatcgaaga catcatccaa atgatcaacg
    20221 acatgggcat tcaggtgatg gaagaagcac cggatgccga tgatctgatg ctggctgaaa
    20281 acaccgcgga cgaagatgct gccgaagccg ccgcgcaggt gctttccagc gtggaatctg
    20341 aaatcgggcg cacgactgac ccggtacgca tgtacatgcg tgaaatgggc accgttgaac
    20401 tgttgacccg cgaaggcgaa attgacatcg ctaagcgtat tgaagacggg atcaaccagg
    20461 ttcaatgctc cgttgctgaa tatccggaag cgatcaccta tctgctggaa cagtacgatc
    20521 gtgttgaagc agaagaagcg cgtctgtccg atctgatcac cggctttgtt gacccgaacg
    20581 cagaagaaga tctggcacct accgccactc acgtcggttc tgagctttcc caggaagatc
    20641 tggacgatga cgaagatgaa gacgaagaag atggcgatga cgacagcgcc gatgatgaca
    20701 acagcatcga cccggaactg gctcgcgaaa aatttgcgga actacgcgct cagtacgttg
    20761 taacgcgtga caccatcaaa gcgaaaggtc gcagtcacgc taccgctcag gaagagatcc
    20821 tgaaactgtc tgaagtattc aaacagttcc gcctggtgcc gaagcagttt gactacctgg
    20881 tcaacagcat gcgcgtcatg atggaccgcg ttcgtacgca agaacgtctg atcatgaagc
    20941 tctgcgttga gcagtgcaaa atgccgaaga aaaacttcat taccctgttt accggcaacg
    21001 aaaccagcga tacctggttc aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac
    21061 tgcacgatgt ctctgaagaa gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag
    21121 aaaccggcct gaccatcgag caggttaaag atatcaaccg tcgtatgtcc atcggtgaag
    21181 cgaaagcccg ccgtgcgaag aaagagatgg ttgaagcgaa cttacgtctg gttatttcta
    21241 tcgctaagaa atacaccaac cgtggcttgc agttccttga cctgattcag gaaggcaaca
    21301 tcggtctgat gaaagcggtt gataaattcg aataccgccg tggttacaag ttctccacct
    21361 acgcaacctg gtggatccgt caggcgatca cccgctctat cgcggatcag gcgcgcacca
    21421 tccgtattcc ggtgcatatg attgagacca tcaacaagct caaccgtatt tctcgccaga
    21481 tgctgcaaga gatgggccgt gaaccgacgc cggaagaact ggctgaacgt atgctgatgc
    21541 cggaagacaa gatccgcaaa gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc
    21601 cgatcggtga tgatgaagat tcgcatctgg gggatttcat cgaggatacc accctcgagc
    21661 tgccgctgga ttctgcgacc accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg
    21721 gcctgaccgc gcgtgaagca aaagttctgc gtatgcgttt cggtatcgat atgaacaccg
    21781 accacacgct ggaagaagtg ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga
    21841 tcgaagcgaa ggcgctgcgc aaactgcgtc acccgagccg ttctgaagtg ctgcgtagct
    21901 tcctggacga ttaatcggta ggccggatca ggcgttacgc cgcacccggc actaggccct
    21961 ctgcacaaac gccacctttt cggtggcgtt ttttatcgcc cacgcactac cagcgcctgg
    22021 tccagctcgc gatacgcttc aaccagtttc tccagtgaaa cgcgacttaa accgctggga
    22081 tttggcagca cccaaatctg cgtcgaacca atggtgagcg tttgtttccc ccactgtgca
    22141 ccgcgctggc tgaatccctg ttcatatgct tgtttgccca gaatcgccaa cgcctgcggc
    22201 tgataatctt caattttttc aatcagctta cgcccgcctg cgtgtagctc ctgctttgaa
    22261 acttcattgg cttgcaccgt tggacggtct accagtttgg tgacgccaca acgataatcc
    22321 agcagatgct gtgcctcctg cggcttcaac tgacggtcgg taaacccggc ctgatatatc
    22381 accttccaga agcgatttgc cggatgagca aagggaaaac cagtcccggc ggatgaaagc
    22441 ccagggttga taccgcaaaa cacgacccgt aaccctggag ccaaaatatc ctcaaccata
    22501 tttactcctt tctaacatca ccaggaaaag tataacggat tgaaaataca ttgtttataa
    22561 aaacagcagg cgcgcggtaa tggctggatt gcgacacgga gttactttat aatccgctac
    22621 catggcccct tagctcagtg gttagagcag gcgactcata atcgcttggt cgctggttca
    22681 agtccagcag gggccaccag atatagcaaa ggctgacgag aaatcgtcag cctttttctt
    22741 tttatatatc agttactttg cgtgccagta agccgctgca cgtacccgct gtgggtcata
    22801 ctgttccgct tcaaagcggc ggcttaaatt cttaacgact ttaccttcgc cggttatcca
    22861 gatgaagtaa tcatcggcag ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc
    22921 atcatgtgcc agccattcga tattaaaacc atcaaggtgc gcgagataat cctgacaggc
    22981 gttatcccgc acgctaacca gcgcactaac ttgcggttta acggcaagtt tgctcaacat
    23041 ttccaggcgg cggcgcaatg cgggcattcc ggattcatcg cagacataca gctgatacgc
    23101 gtaatcttcc ggcaccacca gcgaaccgcg cggacctgcc accgtaagtt tatcgcccgg
    23161 ttgcgcctgc atcgcccagc cgctggcgac cccaccgtcg tgaataaaga aatcaatcac
    23221 cagttcatgg cgtagttcgt catacagcgg cgtatagtca cgcgacggtg ggcgtggtcc
    23281 ttccggccag acgatgccct cttccgttac cgttggcggc acaaagtgag cgtcaggttg
    23341 aggaaagaag agtttgctgt gatcgtcaaa gccacgcgat gtaaaaccgt ccagcgcctc
    23401 gccgccgagg acaatgcgct gaaaaccggc gctgatgcgc tcaacgcgta acacagtcag
    23461 ttcacggaag cgcagatcat tgcgaacgcg ctgcgggtag cggggggagt tattcattgt
    23521 tatcgccttc gtgatggtaa ttagatatat ctaaataaa
//
LOCUS       DAPHJE010000069        23539 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPHJE010000069 DAPHJE010000000
VERSION     DAPHJE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.069"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            35..751
                     /locus_tag="RH222_002769"
     CDS             35..751
                     /locus_tag="RH222_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3797761.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            781..1281
                     /locus_tag="RH222_002770"
     CDS             781..1281
                     /locus_tag="RH222_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3797762.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1373..4057
                     /locus_tag="RH222_002771"
     CDS             1373..4057
                     /locus_tag="RH222_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDV3797763.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4054..5142
                     /locus_tag="RH222_002772"
     CDS             4054..5142
                     /locus_tag="RH222_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDV3797764.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5230..6528)
                     /gene="exuT"
                     /locus_tag="RH222_002773"
     CDS             complement(5230..6528)
                     /gene="exuT"
                     /locus_tag="RH222_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV3797765.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7011..8423
                     /gene="uxaC"
                     /locus_tag="RH222_002774"
     CDS             7011..8423
                     /gene="uxaC"
                     /locus_tag="RH222_002774"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV3797766.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8438..9925
                     /gene="uxaA"
                     /locus_tag="RH222_002775"
     CDS             8438..9925
                     /gene="uxaA"
                     /locus_tag="RH222_002775"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV3797767.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10008..10559
                     /locus_tag="RH222_002776"
     CDS             10008..10559
                     /locus_tag="RH222_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV3797768.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEVSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10564..11808)
                     /gene="sstT"
                     /locus_tag="RH222_002777"
     CDS             complement(10564..11808)
                     /gene="sstT"
                     /locus_tag="RH222_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV3797769.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12207..13172)
                     /gene="alx"
                     /locus_tag="RH222_002778"
     CDS             complement(12207..13172)
                     /gene="alx"
                     /locus_tag="RH222_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV3797770.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13455..14441)
                     /locus_tag="RH222_002779"
     CDS             complement(13455..14441)
                     /locus_tag="RH222_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3797771.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14520..15203)
                     /locus_tag="RH222_002780"
     CDS             complement(14520..15203)
                     /locus_tag="RH222_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV3797772.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15280..15783)
                     /locus_tag="RH222_002781"
     CDS             complement(15280..15783)
                     /locus_tag="RH222_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV3797773.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15868..17004
                     /gene="rlmG"
                     /locus_tag="RH222_002782"
     CDS             15868..17004
                     /gene="rlmG"
                     /locus_tag="RH222_002782"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV3797774.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17195..17278
                     /locus_tag="RH222_002783"
                     /pseudo
     CDS             17195..17278
                     /locus_tag="RH222_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17275..17691
                     /gene="higA"
                     /locus_tag="RH222_002784"
     CDS             17275..17691
                     /gene="higA"
                     /locus_tag="RH222_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HDV3797775.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17736..19754)
                     /gene="fadH"
                     /locus_tag="RH222_002785"
     CDS             complement(17736..19754)
                     /gene="fadH"
                     /locus_tag="RH222_002785"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV3797776.1"
                     /translation="MSYPSLFASLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSAFQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20080..22431)
                     /gene="ygjK"
                     /locus_tag="RH222_002786"
     CDS             complement(20080..22431)
                     /gene="ygjK"
                     /locus_tag="RH222_002786"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV3797777.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDHLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22448..23518)
                     /gene="ygjJ"
                     /locus_tag="RH222_002787"
     CDS             complement(22448..23518)
                     /gene="ygjJ"
                     /locus_tag="RH222_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV3797778.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMIVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5922 a         5997 c         6070 g         5550 t
ORIGIN      
        1 ctttttggaa acaatattgt aagatcaaat ttatatggtg gcgaaaatta atctcttatc
       61 acggcttacg ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa atatgactgt
      121 ttatcctatg gctgtttcca ttaatagtca gggtgaaggc aacgttcgcg taatatctaa
      181 aagtaatgaa gttcagtaca tcaaggcgac agtattccgt atcgataatc catcgacgcc
      241 tcaggaaaat gaagttgaga ttaaatccgg tgatgcaaat catctggttg ttatgccacc
      301 taaattcgct ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga tggaaccaga
      361 gcaaaaagag aaaaattatc gcgttaaatt tgaagcggtt cccagtattg atgacgttgc
      421 cacagataaa aaagatctct ctatgcagtt aacagttaac ttaatttggg ggattgttgt
      481 tagtgttcca cctcagcaac ctattgctaa gttagaagta aatgctgccc aaaaattagt
      541 taatgcaggg aatcaacgct taaagatttt aacgatcgct tattgtaaaa ataatagcaa
      601 agaaaattgt aagatacaga ccgtaaataa aaacatcttc cctggtcagg aaagaaatct
      661 tgaaagcata tctggctacg acaaaatcgt tgtcaaatat aataactgga tcaccaaaga
      721 taatggcgag tttgaactgg cggtccatta attatcattg taataaacga aggaatagta
      781 atgaaaaagg tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc
      841 gcattggcag tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg
      901 acacagaccg ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag
      961 ggtctgcaat cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta
     1021 aaaatgcagt tagtgtcccc agcgcaactg gttcaaagcc tggatgctag caagattgtt
     1081 ccgctgacag taacctgggg cggtgaagaa attaaagctg atgcggcaac cacttttacc
     1141 gcaaccaaaa tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg
     1201 tttgctcaaa ccaccaaagg tgttctggag acaggcatct accgtggtgt agtaagcatt
     1261 tatctgtctc aggatatcta atccagataa cagaattaac acgtaacaaa gggaggggta
     1321 gccctccctt attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa
     1381 aaaattactg gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa
     1441 aattcccgca gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt
     1501 ttcagggaaa agtctcggta aatattatgc aatggttaat cttgataccg taacatttct
     1561 tgatccagca agtctatata acaagctgga gctggatgta gacgatcaga aaatcgcgca
     1621 tatagtgaaa gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta
     1681 tgtacgtact gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa
     1741 tgatgaagaa agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc
     1801 gaagtcatta tatttaaatc cagacaaaaa tactgttaat gcttttatac atcagcaaga
     1861 catcaatgtt ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct
     1921 gggaataaca gaaaatagct atattggtgc acactggaat ttcaacggtt atgatgcaga
     1981 tgatgtcagt gacagtaatg ctgacgtcag cgatctctat tatcgttatg attttttacg
     2041 tcgttattat gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg
     2101 gaactttacc tttaactttc tgccactcgg tgcaattgat gggatgcgta tcgggtcgac
     2161 actgagctat ttaaaccagg cgcaaagcca gcaaggaacc ccagtaatgg ttctgctttc
     2221 gcgcaattct cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa
     2281 tagtggttcg caatttattg ataccagttc ctttccgcct ggcagctaca gcgtggcatt
     2341 aaaagtctac gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg
     2401 cggtctgact gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt
     2461 ttctgatgat gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata
     2521 tgagctctac gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa
     2581 ctggacggca gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa
     2641 cgatgacggc ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac
     2701 gttgggcttt tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag
     2761 ctataacgcc ttaagctgtt atgaaagtat ttccgcgacg gtttcacaga attttgtcgg
     2821 ctggaatatg atgctgggtt atacccgcac acaaaataac actgatgata gtttgcgttg
     2881 ggataaacag cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc
     2941 tgaaactgta caacttagcg cttcccgcgc ttttgtgatg cgtgactgga ttttgagtac
     3001 gtcagtcggt gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta
     3061 cttatcgttt tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac
     3121 aaatgtgtct acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt
     3181 atcgcatacc ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg
     3241 cctgaacacc gataccataa acagcgcggt taacgggcgt tgggatgggc aatacggaaa
     3301 tgtctacgct accgtatctg atagttatga ccgtaagaat catgatcatc tttcggcctt
     3361 tacgggcact tatagctcta cgctggctgt cagtcgctat ggcgttaatt tgggagccag
     3421 tggtacagac gatttgctgg gtgcggtact ggtggatgtg aaaggcttct ctgaacagga
     3481 tgaagagagt caggatctgc aactcgaagc gcgggtggca ggcagccgaa ctttgcagct
     3541 tggtcaaagt gacagtgtgt tgttccctta tcctggattt cagtctggtt ttgttgaggt
     3601 taacgacagt agccagggca atcagcaggg gacaacaaac atcattaacg gtgcagggaa
     3661 tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg ccagctttaa
     3721 ctacaactat atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc cgattgtggg
     3781 gctgaacagc gccatgttac tggtagctga agatggcgga tttacacttg aaattaacgg
     3841 cagcgaaaaa gagctctatc tgctttccgg gcagcaattc cttaagtgtc cactaagtgt
     3901 tgtaaagaaa cgcgccagca ttcgttacag cggagatgtt acttgtagtg tggtgactta
     3961 ttcacaatta ccggagtcga ttcaggttca ggcacagttg aaacagccta aattacgtgg
     4021 aaacgttcag acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcggcgata
     4081 ttgggcttgt ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt
     4141 acgtatgatt tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca
     4201 ggaaataatg gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca
     4261 gctaatggaa atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt
     4321 tggccggtaa aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcca
     4381 ggatgcaagg ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt
     4441 acagaagatg gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag
     4501 cagacagaga tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat
     4561 tcgaacagcc cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac
     4621 catattgacg tgttcttccc ggagttcgcc cacgccacgc caagggtgca gttagacttg
     4681 catccaacag gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg
     4741 tgcctgtacg atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa
     4801 gggcgaccag ctgcagggcg cagagacggt tacttctcta tctatcgtca gggagggacc
     4861 accaccgacg agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt
     4921 gggcaaattg atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaacgt
     4981 gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg
     5041 actctggcag tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa
     5101 ttgtcagtaa tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa
     5161 cgcttttttc tatataaacg acgaagccgt ctgataaagc ggcttcgtca ttacaactta
     5221 agttaaacgt taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc
     5281 cggtttgttc tgcaacacgg tccagataac cagcgcacct aacaggtcaa atactgccag
     5341 aactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa
     5401 cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt
     5461 acgaccgaac acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa
     5521 accaccgata cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc
     5581 cgggccaatc atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt
     5641 cacaccaaac caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc
     5701 agcaaacagc atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac
     5761 tttaaacatg aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa
     5821 acgcggcagc gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt
     5881 cttcgccgtg ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc
     5941 atcggtcaga tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca
     6001 gataaagctc aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc
     6061 ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc
     6121 taccgcgatg gaacgctctt tcgccggaaa ccattcggag ctggctttca gacccgccgg
     6181 gatcatcgct gcttccgcgg caccgactgc accacgagca atcgccaggc caccccagct
     6241 accagccagc gcggttgcac cacagaatac ggcccacagc acagcaaaca ttgcataacc
     6301 gattttcgta cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc
     6361 agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat
     6421 cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag
     6481 cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat
     6541 cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt
     6601 tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg
     6661 ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt
     6721 tgcattccgt cgacttatga tttgcgacgg cagaaagata acttgtcata caactttaaa
     6781 aggtgagagc catcacaaat gtgcgaatat ttgtagggac attacctgac gacagcgagg
     6841 ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg
     6901 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     6961 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     7021 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     7081 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     7141 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     7201 ctatgcgtac caacggtgtg gctgagcgtc tgtgtactgg tgatgcgtct gaccgtgaaa
     7261 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     7321 cgcacctcga actgcgtcgt ccgttcggta tcactggcaa attgctttct ccgtcaactg
     7381 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     7441 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     7501 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     7561 ggcgtccgga caaagccttt aacattgaac aggcgacctt taacgactac atggcgaagc
     7621 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     7681 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     7741 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     7801 gcgaacccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     7861 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     7921 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     7981 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     8041 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     8101 gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     8161 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga
     8221 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     8281 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     8341 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     8401 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     8461 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     8521 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     8581 tatcgcaaaa ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga
     8641 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     8701 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     8761 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     8821 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     8881 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     8941 actgggcgac gatcacatca acacccgcac catgctgcaa aacatggtgc gccacccgaa
     9001 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     9061 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     9121 tgatgagatc gaagccggaa tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     9181 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     9241 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     9301 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     9361 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     9421 caaacagtac tttattgccc atgaccagcc gatctatgaa aacccatcgc cggggaacaa
     9481 agcgggcggt atcaccacgc tggaagataa atcccttggc tgtacccaga aagcgggttc
     9541 cagcgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccgg ggctgaactt
     9601 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     9661 tatggtgctg ttcagtactg gtcgtggcac gccatacggc ggctttgtac cgacggtgaa
     9721 aatcgctacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     9781 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     9841 tgagtttgcc aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat
     9901 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     9961 ataatgtgaa gcgccgtttg cttttccata cctgctaagg atttatcatg accgcgtatt
    10021 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
    10081 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
    10141 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
    10201 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
    10261 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
    10321 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgta atgtggtttt
    10381 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
    10441 aggttagttt tctgcttatg aatggcctga atatcattcg tttctggcgg atgcagaaaa
    10501 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
    10561 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
    10621 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
    10681 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
    10741 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
    10801 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
    10861 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
    10921 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
    10981 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
    11041 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    11101 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    11161 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagcccaaa
    11221 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtaacgg cattcgacat
    11281 atcgttaacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaagc cgaggccgat
    11341 cgcccacacc agaatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    11401 catgctcatt accagcccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc
    11461 cgcgctactg gataaatgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag
    11521 agcagcagag aaggtgccca gtagatagag gaacaaaata gggcggatat tggttttctg
    11581 cccgtgctgg tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac
    11641 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    11701 tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    11761 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    11821 tcattgtgtt gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    11881 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    11941 tttaaagaga ggtcatattt tgcaacttaa tggttacatt tatttgacat aaaaataaag
    12001 ccaacgtgcg gcttccagac tgatgacaac atcaaaaaag gactcggtca gtcccctcgc
    12061 cccgtttggg gagagggtta gggtgagggg aacaggccag catcggtgcg aaaaattacc
    12121 ctcaccccga ccctctccct ggaagggaga gggagaaaac ggtgctcatg ttaatgacag
    12181 ggtacacggc ttaggcagat taaaaattat ccaccccgct gcttatcatg ccgataattc
    12241 acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc cagcgagacg
    12301 gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa caccaggatc
    12361 accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag caggaaatac
    12421 atcgcacgca ggccgaggat cgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg
    12481 gtcacggcga agatagccgg aatgctatcc acggcgaaaa tcacgtcgct caattccacc
    12541 agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg cacaaagaaa
    12601 tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc
    12661 ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc
    12721 aggaaggcac cgaagatata caggatccag tcgaactgcg aaattagcca gctgccagtg
    12781 aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag cacgcggcgt
    12841 tgtaatgccg ccggaacaga gaaatagctg aacaacatca gccagacaaa gacgttatcg
    12901 accgccagcg atttctcaat cagataacct gtgagaaagg ccagtgcctg tggatcggca
    12961 acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa cagtaacgac
    13021 agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc atgtgcccca
    13081 cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac agcgaatccg
    13141 ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata aaaaaacggc
    13201 caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc cggcaaggtc
    13261 tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc ggtgaccgga
    13321 tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc ccctttgcag
    13381 gtaacaaagt atgacagacc attcagccag tcaatggtct gttacatacc ttttacactg
    13441 ttttactttg gcaattatag ttttacgcta tctgccggaa aaatcacccc agtctggcgg
    13501 cggatctcgg tcagcagttt ggcggtgatg cgactgaccg ccagccccgg atgatccacc
    13561 agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt attaatatgc
    13621 tgcggctggg tgagatcctg catttggctg ccacgcggca cgaagcacac tttctggcat
    13681 tcagacagtt tttcaatcac cagcgatcct gcttcgccct gaatctcgct cgccaggaca
    13741 gaatcactga cttttgagtg ctgcaaggtg acgctgaaat caccgtaatc catcaccacc
    13801 acgccgtggg catctacacc gcttgccagc aaactggcgg ttgcctgcac gcttttcggc
    13861 tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat aattgaaccg
    13921 ttagagaatg acggattaaa ggtgttggga ttttcaccgt ccaggtaacg ttgatagcgc
    13981 gaggaatact ggcaatagtt gaaaaagact ttacgcaatt tgccgacttt cggcagcgcc
    14041 tggcgcagca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa cagcaccacc
    14101 tgattttccc gcgcacaggc aatggcggca tccacttccg ccagattcga cgccagtggt
    14161 ttctcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga aaagtgcagg
    14221 gcattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat cgcttccagc
    14281 gaggtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg
    14341 cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc gacgaactgg
    14401 cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg atactccata
    14461 aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc agatacgcat
    14521 tattcttttg ccggacagcc atgctcacta aacggcccaa tcatgaccga tggcccgaga
    14581 tatttcggct ggcgatggag gattttggcg tcaatcacgg cgctgacacg agcgagtttc
    14641 tcccgcagtt gagtataaaa accgtgcttc atattgaggt caggcgcgtt aaaaccgata
    14701 gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt gattatgaaa ttcttgcgag
    14761 ataatggtga tatggttttc accaaaaacc tttttggcac gcactaccga atccagcgtt
    14821 gagaatccgg catagtcgca gaagatcact ttcgccggta cgcctttagc gatcaaagcc
    14881 tgctgcatac ctgaggcttc atcgtaattt ttacgtccgt tatcgccact taccagtagc
    14941 catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg ggtaaagtag
    15001 cgattacccg gcctggcccc cagcaataag cccacgttac gcgccggaac tgcgttgaca
    15061 tcgctccagg tcagctgctt gctggcgttg accataacgc gatcggcaat aaaaatggtc
    15121 gctcctgcta taagcaacaa caaacagcca atcttaagag tccggcgaga aaaacaaata
    15181 cggagaagaa ggcggggctg catggtcgtt atccttaacg aaagggggga ggtcgatttt
    15241 aggcgctaaa gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag
    15301 cgacaactgc gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat
    15361 atggcagcac aactggtaaa acgctttgtt atgctctttc tccttcaggt gcgccagttc
    15421 gtgcaccacg atcatacgta aaaacggttc cggcgcgttg cgaaacacag tagcgacgcg
    15481 gatctctgct tttgctttca gcttgccgcc ctgcacacga gaaacagcgg tatgtaaccc
    15541 gagcgcgttt tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc
    15601 attgcgcaga aactgatttt ttagatcctg agtatattgc cagagggctt tatcggtggc
    15661 gtagtcgtgt gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat
    15721 cagcgttcgc acctgggaaa gtagctgctc gggataaccc tgaagataag taagattgct
    15781 cataaccgcc cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg
    15841 tcgaaatttt atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg
    15901 acactacaac gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggcg
    15961 gatgaatatt tgctgcaaca gttggacgac acagaaatcc gcggcccggt gttgatcctg
    16021 aatgatgcct ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc
    16081 gactcataca tcagtgaact ggcgacgcgc gagaatttac gcctcaacgg gattgatgag
    16141 tcgagcgtga agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg
    16201 atcaaagtgc cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg
    16261 gtcacgtcgg atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg
    16321 ctggagctgt tcgagaaagt gctcggcccg accaccacta cgctggcatg gaaaaaagcg
    16381 cgcctgatta attgcacttt caatgaaccg ccgctggccg atgcgccgca gaccgttagc
    16441 tggaagctgg aaggtactga ctggactatc cacaaccatg cgaatgtctt ctcccgcacc
    16501 gggctggata ttggcgcacg cttctttatg caacatctgc cagagaatct cgaaggtgag
    16561 attgtcgatc tcggctgtgg taacggcgtt attggcctga cgctgcttga taaaaacccg
    16621 caggcgaaag tggtgtttgt cgatgaatcg ccgatggcgg tcgcttccag ccgaatgaat
    16681 gttgaaacca acatgccaga ggcgttggat cgctgcgagt ttatgatcaa caacgcgctc
    16741 tccggagtgg agcctttccg ctttaatgct gtgctctgca acccgccgtt tcatcagcaa
    16801 catgcgctga ccgataacgt cgcctgggag atgttccacc acgcccgccg ctgcctgaaa
    16861 atcaacggcg aactgtatat cgttgccaac cgtcacctgg attacttcca taaactgaag
    16921 aagattttcg gcaactgcac caccatcgcc acgaataata aatttgtggt gctgaaaaca
    16981 gtgaagctgg ggcgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgttaacgcc
    17041 ttatccggcc tacgttcagc acgttactcc caggccggat aagacgcggc aagcgtcgca
    17101 tccggcaatg tctgcccagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac
    17161 catcctctac attctcttgt ttagcgttgt tcaacgttta tgctacatac gtgaagttat
    17221 gacgcataaa gaatacgatt tctttaccgc tgttcatcgt actaagggga aaaaatgatt
    17281 gcgattgccg acatcttgca agcaggagaa aagctaactg cggtggcacc ttttctggcg
    17341 ggtattcaga acgaggaaca atacacccag gcgctggaac tggtagatca tctgctgctc
    17401 aacgatcctg aaaacccctt gctggatctg gtgtgtgcca aaataaccgc gtgggaagaa
    17461 tcagcgcccg aatttgcgga atttaatgcc atggctcaag ccatgcctgg cggtatagcc
    17521 gtgattcgta cccttatgga tcaatatggt ttaacccttt ccgatctgcc ggaaattggc
    17581 agtaaatcta tggtgtcacg cgttttgagc gggaagagga aattaacgct ggaacacgct
    17641 aaaaaattgg caacgcgatt cggcatttct cccgccttgt ttattgatta acgcgacgtg
    17701 ccggatgcgt tgcatccggc gcttcaaata acgatttaaa tctccagcgc cagccgtgtt
    17761 ccctgggcaa ttgcccgtcg tgcgtccagc tccatagcca catcgcagcc gccaattaaa
    17821 tgcacagttt tcccgctatc aatcagcggt tgcgccagcg cgcggtttgg ctcttgccct
    17881 gcgcagatca ccacattgtc cactgctaat acctgcgttt cgccgttgat caccacatgc
    17941 agcccgtcat cgtcaatctt ctgataactt acgcttggga tcattttcac accccgcgag
    18001 agcagggtgg tgcgatggat ccagccggtg gttttgccta acccctgtcc cggtttgctg
    18061 gctttgcgct ggagcatcac aatttgccgt gggctacgcg ggatctgcat tccctgcggg
    18121 cttaagccac cagcctgttg taggctgctg tcgatccccc actcattaca gaacctggcg
    18181 atattctggc tggtggattc gcccggctga cttaaataca tcgccgtatc aaaaccaatc
    18241 ccgccacaac cgatgatggc aaccttgttg cctaccggcg ctttgtcgcg cagtacatcg
    18301 agataactca ataccttcgg atgatcgatc ccgtcgatag gcggaacgcg cggcacgatc
    18361 ccactggcga ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg
    18421 agtttcagcg tcacgcccgt cacttcgatc atccggtggt aatagcgcag cgtttcgtaa
    18481 aactcctctt tgccggggat ctgtttggcg atattaaact gcccgccgat ctcgctgtgg
    18541 gcatcaaaca atgttacctg atgcccacgc gccgccgcgt taatggcaaa cgccagccca
    18601 gcaggtcccg caccgaccac cgccagattt tttttctgca cggcaggaag gattggcatt
    18661 ttggtttcgt ggcaggcgcg aggattcacc aggcacgagg tgactttgcc aacgaagatc
    18721 tgatcgaggc aggcctgatt gcagccaatg caggtgttga tctcatcggc tcgccccgat
    18781 tgcgcttttg acagcagttc cgcatcagca agaaacggtc gtgccatcga caccatatcg
    18841 gcatcgccgc gcgagagaat gtcgtcggca acctgcggat cgttaatccg gttggtggtc
    18901 accagcggca gcgagacgtg gcctttcagt ttgcgcgtga cccaagtaaa tgcgccgcgc
    18961 ggcacgggcg tggcaatggt cggaatacgt gcttcatgcc agccaatgcc ggtgttgata
    19021 atggtcgcgc ccgccgcttc aatggcctgc gccagttcta ccgtttcggc aaaagtgccg
    19081 ccgccttcga ccaggtcgag catcgacaac cggtagataa taatgaagtc gttgccgacg
    19141 cgttcgcgca ccgcacgcac tacttctacg gcaaatcgca tccggttgcg gtaatcgccg
    19201 ccccactggt cactacgctg attggtgcgc agcgtcagaa attcgttgat caaataccct
    19261 tcggaaccca tcacctctac accgtcgtat cctgcctccc gcgccagttg cgcgcagcgg
    19321 gcgaaatcgt cgatcagttg caggatctct tcatgggtga gttcgtgagg aacgaaacgg
    19381 ttgatggggg cctgaaatgc ggagggagcg accagatgcg gttggtagct gtagcgcccg
    19441 gtatgcaaaa tttgtaaggc aattttgccg ccttcctgat gtacggcttc ggtaatggtg
    19501 cgatggtgtg ggatctggct ggcgtcattg agcattgcgc caccttccat gccgacgcct
    19561 gttaaatctg gtgcaatgcc gccgctaaca atcagcgcca cgccgtgacg ggcgcgttcg
    19621 gcataaaacg cagccagccg ttcagcaccg tccgggtatt cctccagccc ggtgtgcatt
    19681 gaacccatca acacgcggtt ttttaacgtg gtaaaaccta aatccagcga ggcgaacagc
    19741 gacgggtagc tcataaaatt gtccagtatg taaaataatt gttatgtggt cggatgagtt
    19801 ctaatttagc cgtcgccgga gaaaagggaa aaggggagtg cggggtttgt gatgggattc
    19861 aaaaagaata cgcccggtgg taacgccgga tgcgacgctt gccgtgtttt atccggcctg
    19921 ggggattgca catgtagggc ggataaggcg ttcacgccgc atccgccagt ggcgcggtgc
    19981 agttgccgga tgcaacgctt gccgcgtttt atccggccag gaggattgca catgtagggc
    20041 ggataaggcg tttacgccgc atccgccagc agttgcaaat tattgcttac ggaaaaaatc
    20101 gttatacaac atatacaaat gcgcggcact ccaggagaaa tttggtgcgc cttgctgtgc
    20161 gcctgtcagc gggttgtaat tttcctgaat tggtccatcg gcggttaacc ctttggcgtg
    20221 ccggaagaac gtatccgcca gcttcagggc atcatcacga taaccgtaac gctccatccc
    20281 tttcagacca aaccagaact gatccaccca tacgcgccca cgccagtaga tatcagcgcc
    20341 aaaagccgga ttggttaacg ccgccgttcc cagcgggaca aaggtgttga actctttagg
    20401 atcgagcatc accttcacca ccgcgtcggc gttggcctgc gttgccgcac cgttaaacag
    20461 cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc ggtttgcccg cgcagccgtt
    20521 cgccagcggt ttatcttcaa tacgcacgtc atagtagaac tgggtagtcg ggtcgaacat
    20581 acaggtgttg atgtagtccg cgagctgctg tgccaactgg cgatagcgtt tggcctcttc
    20641 tggcttaccg aggatggttg ccatctccgc cagataatgg ttatcgctgt acatatagct
    20701 ggcctgatcc accgactcct gcaatagcga gtagcccagc aacgttccgt cctgactgcg
    20761 gttttcggcg aatttcaccg tccagtcgct acgtttgccg ccattagcga catatttatc
    20821 caactgttct ttgtcgataa acccaaagac ggcggcatca tcacgccccg attcccacga
    20881 tgcagcaacc tgtgccggaa tttccagact gtcgtactgg cctttctcca ccacgcgggc
    20941 gtagttgttc agcccagact gcgtctcttc tttatcgcct tttttcaccg taaacagcat
    21001 ctcgccgctc tcagtgttgt gggctttgtc gcgggtcgcg ccatattccg gcacgccgtt
    21061 gccgttgtga tcgcggttac gtaaccacca gtcgtgatag gccaccagtt tcgggtacat
    21121 ctctgccacc caggttttat cttgggtgac gttatacact tccatcaccg accaggcggc
    21181 aaggctgggt ttggtattac gttcgttcca gttgccgcca tcgccaccac gctcggggct
    21241 aaggttccac gctatcaggt cggggacaaa gcccacatcc tgcggacgca cgcggtcgcc
    21301 tggctggatc tgccaggaga agaccgcgcg gatattctct ttggcgatgt ccggattgaa
    21361 atgcgccatc gcaaacgcct gcttccaggt atcccacggc caggtctgat tgccggagaa
    21421 ccagcgcccg gtcaccgacg gtgtgacggt gttaaatttc accgccccgc caggcgaacg
    21481 ccagttaccg ttgagcgttt cgatcgcttt caccgcgacg cgcgtctgtt ccggcgtcgc
    21541 atccggattg gttaaccctt tcttcagata ctcttcccag cgttgctgcg aggcggtgag
    21601 ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt tgctctttgc taacttcctg
    21661 agaggtcagc agatgggaat aggtggtata gagcgtcgtc gaaccgttga tatgtgcctt
    21721 actggtaaag cgattgccgt tgatttcagt ctgcaccggc agggatttat gcacctgata
    21781 ttctgattca ccggaggtca gcagatccca ggtggcgcgc actttgccaa aggtgacttt
    21841 caggccatca cgggtggcgc tgattttgcg ctggtagtca gggtattcgc cagcaatggt
    21901 tttatcggaa agcggtttcc cttctttcgc ttccagtttt tccagcagtt cgccatccca
    21961 caccagatcc agcggtttat tgctgatgat tttagtttcc agtagtgacg tgcgcggcgt
    22021 ggcgaagcgc agagtcattt cgacctgcac atcttttgct gtcagttttt gcaccagcgc
    22081 gccgggaata ctgtatgcct ccagcgtgaa gtcgactttc ttgccgtcct gccagacggt
    22141 caggtggtcg aaattgctgg ccataaagtt gatgtactct tccgtcagca gcgcaacgcc
    22201 tggaaaaccg cccatggtgt tagggccgtc tggcaacaga tgaccatgcc aggctccgag
    22261 atcgaaaaac ggattaaaac gctgatgatc gtcgtaatcg taatccttca tgtactgcgg
    22321 cgcaccagta cggttaatca cgtttttata attgtcggcg ttagcggcat gggcggaaaa
    22381 acttatcagc agagcgcagg ttactggcgt taaaatagtt ttgattttca tgaaattaca
    22441 gtccttatta ccagtagaaa tattgcatca ggaagatttg gtcgctggga tcgtgagagg
    22501 catttttcat gtagaagcgg ctatcaaaag ccgttgcgaa acgtccgccg ccgtattcgt
    22561 accagatacc aaactgcatg aaagaggtgg tttcatcgtc agcgtggtca gggcgatgtt
    22621 tggcgtaggt gtaagcggtg gagagataaa cgccctttaa tgcttcatac atggcgctca
    22681 ctatcatccc actttcactt cccggtacgt ccagcccgtc ggcacgtccg gtatggtgcc
    22741 aggcgcgagc ggcaaagtta ggggtaagca ggccggtgag atagagttgt cctgtgccgt
    22801 cggtaatttc cagaccattc atccaggtga tgccttcggc aaggtcatac tggatgtaac
    22861 cgttcatcat tgccggatag gtgtatttgt cgtcgtagcg atcgtacttg ccaaaatgta
    22921 gccaggcttt gctttcgtca tgatgtccgg caggtgtggc ggtgacgcta taacgaaagt
    22981 tgccgcttaa gttttgcacc ttcagcgcag tcataatgtc gcgggtattg ggaatgacgt
    23041 agcccagatc gggcgtgaaa tcgccccacc attgcaggtc atccagcgag gaatcctgac
    23101 gcagactgag catgatttcg gtgccatcat tggtacgata accgccatag aaacggttaa
    23161 ttccgccctc gaaaccaccc cagctatggt caggcaccca ggcattgccg tcgtggtcgg
    23221 cctgcacggt ccagccttcg ccgtaaatca ggccgaacca ggagccgtac ttcatctcca
    23281 ggccaccttc aatataggtg ccgtcactgt atttatgttt attctcaccc tctaaatcca
    23341 tatatccacc aacccctaat tcgccgtaaa aacgaaatga aggagaagat gtcgaggtaa
    23401 tggcgggtgt ttttgtgtcg tcatgttctg ccggacgtgc ttcttctcct gccgcagaaa
    23461 aggcgtagca aaacggcaga gcaagtaatg ctctgcatgg tgcagtgatt aatttcattg
    23521 tgttaatcca tgagctaaa
//
LOCUS       DAPHJE010000070        23405 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPHJE010000070 DAPHJE010000000
VERSION     DAPHJE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.070"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(18..3899)
                     /gene="entF"
                     /locus_tag="RH222_002788"
     CDS             complement(18..3899)
                     /gene="entF"
                     /locus_tag="RH222_002788"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV3797779.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(3896..4114)
                     /gene="ybdZ"
                     /locus_tag="RH222_002789"
     CDS             complement(3896..4114)
                     /gene="ybdZ"
                     /locus_tag="RH222_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV3797780.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(4117..5319)
                     /gene="fes"
                     /locus_tag="RH222_002790"
     CDS             complement(4117..5319)
                     /gene="fes"
                     /locus_tag="RH222_002790"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV3797781.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRGDRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5562..7802
                     /gene="fepA"
                     /locus_tag="RH222_002791"
     CDS             5562..7802
                     /gene="fepA"
                     /locus_tag="RH222_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV3797782.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPLDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7977..8597
                     /gene="entD"
                     /locus_tag="RH222_002792"
     CDS             7977..8597
                     /gene="entD"
                     /locus_tag="RH222_002792"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV3797783.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8719..8871)
                     /gene="hokE"
                     /locus_tag="RH222_002793"
     CDS             complement(8719..8871)
                     /gene="hokE"
                     /locus_tag="RH222_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV3797784.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9324..10442
                     /locus_tag="RH222_002794"
     CDS             9324..10442
                     /locus_tag="RH222_002794"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV3797785.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10508..10756
                     /locus_tag="RH222_002795"
     CDS             10508..10756
                     /locus_tag="RH222_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV3797786.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10821..11189
                     /locus_tag="RH222_002796"
     CDS             10821..11189
                     /locus_tag="RH222_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV3797787.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11283..11936
                     /gene="nfsB"
                     /locus_tag="RH222_002797"
     CDS             11283..11936
                     /gene="nfsB"
                     /locus_tag="RH222_002797"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV3797788.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12044..13291
                     /gene="ybdG"
                     /locus_tag="RH222_002798"
     CDS             12044..13291
                     /gene="ybdG"
                     /locus_tag="RH222_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV3797789.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13359..14735)
                     /gene="pheP"
                     /locus_tag="RH222_002799"
     CDS             complement(13359..14735)
                     /gene="pheP"
                     /locus_tag="RH222_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV3797790.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14837..17980)
                     /gene="cusA"
                     /locus_tag="RH222_002800"
     CDS             complement(14837..17980)
                     /gene="cusA"
                     /locus_tag="RH222_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV3797791.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(17992..19215)
                     /gene="cusB"
                     /locus_tag="RH222_002801"
     CDS             complement(17992..19215)
                     /gene="cusB"
                     /locus_tag="RH222_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV3797792.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19231..19563)
                     /gene="cusF"
                     /locus_tag="RH222_002802"
     CDS             complement(19231..19563)
                     /gene="cusF"
                     /locus_tag="RH222_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV3797793.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19587..20969)
                     /gene="cusC"
                     /locus_tag="RH222_002803"
     CDS             complement(19587..20969)
                     /gene="cusC"
                     /locus_tag="RH222_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV3797794.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21126..21809
                     /gene="cusR"
                     /locus_tag="RH222_002804"
     CDS             21126..21809
                     /gene="cusR"
                     /locus_tag="RH222_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV3797795.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            21799..23247
                     /gene="cusS"
                     /locus_tag="RH222_002805"
     CDS             21799..23247
                     /gene="cusS"
                     /locus_tag="RH222_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV3797796.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5613 a         6374 c         5964 g         5454 t
ORIGIN      
        1 ttatagatat tattaattta cctgtttagc gttgcgcgaa taatcggccc aatttttaca
       61 aacgcccctg gagagataat atccacatgc gcacaatcct gacgatagat atccagctcc
      121 gcaatccacg gcgaccaggc acgttcggga ctcatacctt cctgaagtgt gcgctcagca
      181 acaaataacg ttgcttttcc atcaaacgga acgctatggg cggtggttaa caagcgcaca
      241 gcatcagcgt agttgccttc aatggtggta aacaactccg ttgaagtact tccctgctgt
      301 gccgccagga aggcctcgcg ttcgcggtta atctccgcca gcacttccgg gtccagacca
      361 ttagcctctt tttcctgcca gttttgcgtc tctggcggcc aggtatccag caaaccaaga
      421 aatgccacct gttcgccacg ggcacgcagt cgcgccgcaa taccctgcgc cagcgtaccg
      481 ccaagggaat accccagcag gtaataaggg ccgtgcggtt gttgctcaag taacgttgcc
      541 agatgcgctt cgcagacttc atccaggttt gtcgctgtct gcatgggtcc atgagggcgc
      601 ggcgactgaa tgccaataat cgaccattgt ggatcgagat aacgcgagag cacgctaaac
      661 tgccaggcaa aaccggacgc cggatggaaa caaaacagcg tcgggccatt accttcacgc
      721 aacggcagaa tggtttcgaa tcccatgcgc cgggagctgt cctcttcacc atcaataatc
      781 gttgccagtt tggcgacggt tgacgcgacc atcacctgcc ccggcgtcac ctgacgagca
      841 aactgtcgac ttaactgcgc tgctagtttc attgccagta gcgaatgacc gcccagcgca
      901 aagaaatcag catcggcatc ctgcacgtca caacccagca acgacgcgaa tgccgcggcg
      961 ataatcgttt cactgcccgc tttcggtgca cgtcccgacg cttgtgtctt cagttcaggc
     1021 aacggtaagg ctttgcgatc cagcttgccg ttggcgctaa gcggcaactg cggaagttgc
     1081 agcagaacga ccggcaccat atgcggcggc aatgtttcgc gaagctgtgc ctgtaatgcg
     1141 ctggtatcca acggcagtcc cgattgcgac accagatagc ccaccaactg acgcgcatca
     1201 ccaccggtgg ctgccgcctg gttaatcaca caggcgtggg taactgcctg ttcgacatcc
     1261 ggcagcgcct gcatcacgcg atcgatttcg cccagttcga tacgctgccc gcgaattttt
     1321 agctgatcgt cactgcgccc gaggtactcc accgcgccgt tatccagcca gcgggcgacg
     1381 tctccggtac gatacatccg ttcacctggg gcaaaaggat cggcaataaa acggctggcg
     1441 gtcagatcgg gtcgtccaag atacccctgc gccagttgga tgccggtgag atagagatca
     1501 cccgccacac ccggcggcac cggatgcatc atcgcatcaa gaatacgcag acccgtattc
     1561 cataccggat aaccaatcgg cacactgctg ccgcgcacct gtgccagttc ctcgccaaaa
     1621 gccggatacc agctgacatc taccgccgct tccgtcgggc catacaggtt atgcaacggc
     1681 gcgccggtta actgttgcca ttcgcggcat aaatcagccg gtaaggcctc accactacag
     1741 aaaacctgtt tcaacgtcgc gcaattctgg cgagcggttt gcggcgtcag cgatgcaaca
     1801 aatgccgcca gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc ggcaaagaat
     1861 tgctgcatag cgagcgggtc acggtgcgct tccggttcag ccatcaccag ttttgcccct
     1921 gcgataaacg gccagaaaaa ctcccacacc gagacatcaa aactgcacgg cgttttttgg
     1981 gcaacgacat cttcgcctgt aagcgggtag tgattttgca tccataacag gcggttaacg
     2041 atagccgtct gcccgaccat tacccctttc ggcctgccgg tggagccaga ggtaaagatg
     2101 atataagccg tgtggtgcgg ttgtgaaagt tgcagcggcg cactgccctg gggggtaagc
     2161 ggggcgttat agcaaaggct tgttaaattg ggaacatcgc taaagcgcgg cagttgatcg
     2221 tcggtggtaa ttaacagcga cggacgcgca tcttccagca tcattttcag gcgatcgtcc
     2281 ggatagccgg tatccagcgg tagccaggcc gcgccagctt caactattgc atggagtgcc
     2341 agggtcaaaa agaccgagcg cggtagtgcc accgccacgc tgtcccctgg tttaacgccg
     2401 cgctcacgca gcagattcgc cagcgccacc acctgctcgc gcatttcccg atagctgaac
     2461 aggtaacgcg catctgccag cgccggagca tccggtgttt ttgccgcttg ttctgccacc
     2521 agcgcgctca gcgtggtttc tggaatctca acctgagtgg cgttgatctg cgccagctgc
     2581 gcatactcac ctggcagcat aatatcgaca tcgccgcaca acagcgccgg atccgcggcg
     2641 aactgggcaa tcagcatttt caggcgttca gcatgctgga ttaacgttgg ctcatcgtaa
     2701 cgctgtttat tggcgaggat ctcaatactc aaatcaccgt gtacatccgg gaacagggcc
     2761 agttcaaggt cattaaccgg accggttgcc agggtatggg tttgcgcctg aacatcagga
     2821 atatccagtt ggtaatcaaa taccttgata ttgagtaccg gaccaaacag cggttcatca
     2881 cctgccgctc gcccgctgtc acggacaatt tgttcggcat cgtaacgttg atgacgacgc
     2941 atttttttca gttgtgctgc cagtcgggtt gccagttccg gcagcgtttc ttgcgccgca
     3001 atgtgaatac ccaacggcaa aacgttgagc acgggtccgg tagccgtcag cgccgccgag
     3061 cccagtcgac gcataaagat aaatccggcg gcgtagtcca tacgattgca caatcgcccc
     3121 agccacaagg ctgccagcgc aagggctaaa tcggtacgct gcacacctga aagttgcgta
     3181 gccagctggc ggaattcccc gtcggtaaat tccagtttca ggcgcagaat atctgccgag
     3241 gcgctgcgcc ccggtaaagg tgccggagaa agtgacgcgg gcggcggcag ttgacgacgc
     3301 tgttctgccc agaatgccgc atcacgctgc caggcttcgc tttcgcggta ttgctggtac
     3361 tcttccacta catcagcgaa aggcgtaaat ggcgaagcag gcgttggttc gccacgcagc
     3421 catgtgcagt aaatattggc gatctggcgg gtaatggccg ggaaactgaa gccatcgacc
     3481 agcaaatggt gataacgctg ataccagtac cagcggttat ccgccacctg tatcagctga
     3541 tgaaagacca gtggtttacc gctatcgacg cgcagatctt gttgcaaatc cgcctgcatt
     3601 aatgcctgcg cagtaccgtg cggatcaatg ttggttcgta ggtcgataat ttctggcagt
     3661 tcgaacgtca gcgcatcatc gacccactgc cagacttcgc cgttatcttc cgtaaaacgc
     3721 atccgcagcg tatctgcttg cgctagtccg gcaaccaccg cgcgggccag taatggcgaa
     3781 tcaacctctc cggttaactc aacgtaatgc gccacgctcc aggcggaggg taattctgac
     3841 agtttttctg ccatccagat gccgggctgt gcggcgacca aaggtaaatg ctggctcatt
     3901 gtgcctcctg caactgggta aaattcgtcg gtgtcagagt acgccagtgg gcttccagcc
     3961 actgctggca ggacgcctgt gactgcggct gacacacaat gtcccagcct gccggtaagg
     4021 cgcattgttg cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg taaaacgctc
     4081 cctgcggatc atcgaaggga ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg
     4141 ttgccagagg tcgattagcc cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc
     4201 gccgtcaacc tgacgccaga aaatggattc ttttatgggg tgtaattgcg catacagcgc
     4261 ctgattggcc cgcataatca tcggctcgcg aatacccgct tccagcacaa tgcgcagacc
     4321 ttcggcgcta acttcaccag ctttaagctt ttcaagtaac acgccctctt gctgcccgcc
     4381 ccgatgcggc caccagtacg atcctgactg gcttaataca cagccaaagc gttcaggcca
     4441 gtgcagtccg gcatacagcg cggacagccc accaaaactc tgcccggcaa ccacggtgcg
     4501 atcgccgcga tcgctaaaag gggcaatagc tttcaccagg ggtaataact cttgctgtac
     4561 tgccagccag aaatccgcat tacacggcag ttcgtgggcg cggtgcgtgg tgtcgatagc
     4621 gtcgatcaac acatacacgg cgggaggaag ttgctgacga tgggtcagcg aagtcagcac
     4681 aggccagacg ggcatacttt gcgcccaaaa ttcgccatcg agcaaaactg ccagcggacg
     4741 ttcttctgct gttacatcgc cggtggtaaa aatccataca cgacgtgaat ttttcaaccg
     4801 ttcacttttc cagataattt ctttggctgc tatttctggc gcttgcggac aatcccatcc
     4861 cggttgcaga ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt
     4921 ccagctttgt ggattcagcg gatcggctat tgcctggggt aatagttttc gccagccttc
     4981 acgtaattcg aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat
     5041 aaagcagtag ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc agacatcagt
     5101 acctgcaatt cgctgcatcg actggggctg gctgttctga tgatgatcgg tcacaccagt
     5161 gatgtagatc catacgcgct ttatcggcga gtattcttca gaaccttggg gatcacgcca
     5221 ccagaaagtg acctcaaaca tttcgtcatt cagacgctgc cattccgggc catgtttcga
     5281 ctgccaccag ctctcacttc ctacttttaa cgccgtcaca ccataacccc atgtttactg
     5341 tgcaattttt cattgattgc agaaatatat tgataatatt attgataact atttgcattt
     5401 gcaatagcgt aatggcgcgc cgtgggaagc gcggacattt attaaccaac tgcactgcgt
     5461 gtctttcagg atcaaaggtt ttcgcggtag cgggatgcgt cgtgttgagg acgaccatgc
     5521 ccgacagttg caattcgtgg caaaaatgca ggaataaaac aatgaacaag aagattcact
     5581 ccctggcctt gttggtcaat ctggggattt atggggtagc gcaggcacaa gagccgaccg
     5641 atactcctgt ttcacatgac gataccattg tcgttaccgc cgccgagcag aacttgcagg
     5701 cacctggcgt ttcgaccatc accgcagatg aaatccgcaa aaaccccgtt gcccgcgatg
     5761 tgtcggagat catccgtacc atgccaggcg ttaacctgac cggtaactcc accagtggtc
     5821 agcgtgggaa taaccgacag attgatattc gcggtatggg tccggaaaac acgctgattt
     5881 tgattgacgg caagccggta agcagccgta actcggtgcg tcagggctgg cgtggcgagc
     5941 gcgatacccg tggtgatacc tcctgggtgc cacctgaaat gattgaacgt attgaagttc
     6001 tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc gggcggcgtg gttaacatca
     6061 ttaccaaaaa aggcagcggt gagtggcacg gctcctggga cgcttatttc aatgcgccag
     6121 aacataaaga ggaaggtgcc actaaacgca ccaacttcag cctgaccggt ccgctgggcg
     6181 acgaattcag cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata
     6241 tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc
     6301 gcgaaggggt gatcaacaaa gatattaatg gcgtggtgcg ctgggacttc gcgcctctgc
     6361 aatcactcga actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc
     6421 agaacaccaa ctctgacgct tatactcgct cgaaatatgg cgatgaaacc aaccgcctgt
     6481 atcgccagaa ctactcgctg acctggaacg gtggctggga taacggcgtg accaccagca
     6541 actgggtgca gtacgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta
     6601 ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgatatcgat cttgatgacg
     6661 tgatgctgca cagtgaagtt aacctgccgc ttgatttcct cgttaaccag acgctgacgc
     6721 tgggtacgga gtggaatcag caacggatga aagacttgag ttccaatacc caggcgctga
     6781 ccgggacgaa caccggtggc gctattgatg gcgtgagtgc caccgaccgt agcccgtatt
     6841 caaaagcaga aattttctcg ctgtttgccg aaaacaacat ggagctgact gacagcacca
     6901 tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg
     6961 cgctgaacat atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc atcgcccgtg
     7021 cttataaagc gccgagcctg taccagacta acccgaacta cattctctac agtaaaggtc
     7081 agggttgcta tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagcag
     7141 aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg
     7201 tcacctggtt ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtagggc
     7261 aaaacgcagt cggcaccgat ctctatcagt gggataacgt gccgaaagcg gtggttgaag
     7321 gtctggaagg atcgttaaac gtaccggtta gcgaaacggt gatgtggacc aataacatca
     7381 cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt
     7441 atacgttgaa ctcaacgctg agctggcagg cacgggaaga tttgtcgatg caaacgacct
     7501 tcacctggta cggcaagcag cagccgaaga agtacaacta taaaggtcag ccagcggttg
     7561 gaccggaaac caaagaaatt agtccttaca gcattgttgg cctgagcgcg acctgggatg
     7621 tgacgaagaa tgtcagtctg accggcggcg tggacaatct gttcgacaaa cgtttgtggc
     7681 gtgcgggtaa tgcccagacc acgggcgatt tggcaggggc caactatatc gccggtgccg
     7741 gggcgtatac ctataacgag ccgggacgta cgtggtatat gagcgtaaac acccacttct
     7801 gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca tgccggatgc ggcgtgaacg
     7861 ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gttggcctga
     7921 taagcgtagc gcatcaggca gttttgcgtt tgtcatcagt ctcgaatatg gtcgatatga
     7981 aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc
     8041 cggcgaattt ttgtgagcag gatttactct ggctgccgca ctacgcacaa ctgcaacacg
     8101 ctggacgtaa acgtaaaaca gagcatttag ccggacggat cgctgctgtt tatgctttgc
     8161 gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaacct gtctggcctg
     8221 cggaggtata cggcagtatt agccactgtg ggactacggc attagccgtg gtatctcgtc
     8281 aaccgattgg cattgatata gaagaaattt tttctgtaca aaccgcaaga gaattgacag
     8341 acaacattat tacaccagcg gaacacgagc gactcgcaga ctgcggttta gccttttctc
     8401 tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa
     8461 ctgatgcagg ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc
     8521 atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaagata gtcataacgc
     8581 tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgacca
     8641 cgggcgaaag tgtaaagcag gtgccttacc atcctgacct gacaaccgga tatgcgggga
     8701 ttgctccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat
     8761 attacgttcc tttacggtga actcacacag cgaatctccg accagaagcg taaatcccag
     8821 caccgttaaa cacagcacta tgactgccgc aagggcatat ttcgtcagca tgtttatatt
     8881 gcctccatgc gaagaaggga ctaccatcca aattgttcgg gtttggagtg gcagccccag
     8941 ggttgataga aatatctcct ggggcttcat ctttccagac ctcagagtaa acctgaaacc
     9001 ggaaagcttc aggcacccgc cgttatctta cccttcctca acaaaatagc tgtttattta
     9061 tacagtgagt cctgtttcgg acttctcact cctcatttaa tcaactgaag ctgaacccgt
     9121 ttttttctgc atgactcaat cgtcaaaatt cttcgccacc agtcagcact gccataactt
     9181 gtcacattgc aaaagtagcc aaattgcttc aggcaccatc agttcaccat caccaaaaaa
     9241 aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat actgaataga
     9301 aaacgccaac ataagagaaa cctatgccat tacccgattt tcatgtttct gaacctttta
     9361 ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac ttaagccagg
     9421 actcttcaat gctgattgac gcggttaaaa ataagatcac ggccggagag gtaaagcacg
     9481 atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc aaccaggctg
     9541 ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc agccacagac catcatctgg
     9601 aaatttgcgg cggtggcacg cacccgtttc agaaatggca gcgtcaggag gtatgcgata
     9661 acgaacgcta tcaacgcacg ctggaaaact ttggttatct cattcagcag gcgaccgttt
     9721 ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga cgccatttat ttgctgcacg
     9781 gcttgtcacg atttgtgccg cactttatcg ccctttccgc cgcgtcgcca tatatgcagg
     9841 gaacggatac gcgttttgcc tcctcacgac cgaatatttt ttccgccttt cctgataatg
     9901 gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctatttcgc tgtttgagtt
     9961 acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc agtcctcatt
    10021 ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac gcggtaaata
    10081 tggcgggatt aattcaggcc accgcccact ggttactgac ggaacgcccg ttcaaacatc
    10141 aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgtcgc tatgggctgg
    10201 aaggcgtcat taccgatccg cacactggtg atcgtcgacc gctaacggaa gataccttgc
    10261 gattgctgga aaaaatcgcc ccttccgcac ataaaattgg cgcatcgagc gcaattgaag
    10321 cactgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc gatttcgtcg
    10381 ccgatggcgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg gccggtgact
    10441 aagtcgggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac ttacatttaa
    10501 caatgttatg aaacaccctt tagaaacctt gaccaccgca gcaggcattt tgctgatggc
    10561 tttcctctct tgcctgctgc ttcccgcccc cgcattgggg ctgacgctgg cacaaaaact
    10621 ggtgagcacg ttccatctga tggatcttag tcagctttac actttattgt tttgtctgtg
    10681 gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc gacgctggtt
    10741 ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt gcttattctc
    10801 cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg aaacgcctgg
    10861 cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc gatgttttta
    10921 aaattggcgg caagattttt atgctgtcgt cggagctacg cggcgtcccc tttatcaatc
    10981 tgaagtccga tccacaaaaa tccctgttaa atcagcaaat atacccaagc attaagccag
    11041 ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa atctccgaag
    11101 cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt ctggctaaac
    11161 gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt tttctcgctt
    11221 accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca catggagtct
    11281 ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt gatgccagta
    11341 aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac agcccatcca
    11401 gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt aaagcgcgtg
    11461 ttgccaaatc cgctgccgat aattatgtgt tcaacgaacg taaaatactt gatgcctcgc
    11521 acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag ctggttgttg
    11581 accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg aacgataaag
    11641 gtcgcaagtt cttcgctgat atgcaccgta aagatctgca tgatgatgca gagtggatgg
    11701 caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg
    11761 acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt ggtctgaaag
    11821 agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt gaagatttta
    11881 acgctacgct gccgaaatca cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct
    11941 ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg cataaccctg
    12001 tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg atttaatatc
    12061 ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg tgatgatttt
    12121 cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg tggtactgcg
    12181 gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca ttacccagaa
    12241 taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata ttcaggcggt
    12301 attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg cgcagttgtg
    12361 gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt tgaatctggc
    12421 gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg ggattaaact
    12481 gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc agtcgccagc
    12541 gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggttt ttaaagatcc
    12601 gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga aactgggcga
    12661 ctggctggag atgccgaaat acggcgcgga tggcgcggtg atcgatattg ggttaaccac
    12721 cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt ctctggtttc
    12781 tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta ttaagcgcag
    12841 tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa
    12901 taaagcgcac ttgttaaaac cttatttaac cagccgccat caggaaatta atgagtggaa
    12961 tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc cgaatgacca atattggaac
    13021 ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta aagatatgac
    13081 cttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga tctacgcgtt
    13141 tactaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat tcgatcacat
    13201 atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg gcaatgatat
    13261 tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat cgaatggatg
    13321 agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg cagcgtttta
    13381 aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa gcgcatatca
    13441 tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcaaag atagttgccg
    13501 aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat cgctgcacga
    13561 aaacgcagat gcgccagaca gatcataatc cagttcaaca gcagcgttgc taccaccagc
    13621 gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaatac caccagcgaa
    13681 gtgatcgctc cggaaagcat cagcgagtta atcggcacac cgcgacggct gacgcgagtc
    13741 aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct gttagagtaa
    13801 accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag cgcagaagct
    13861 accacgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt ggatttcact
    13921 tccacccacg gatagagcgc cagtaaaacc accagtgaac cgatgtaaaa cagcaggatg
    13981 cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg cgcttcagcg
    14041 gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc cagcgacaaa
    14101 atcagcccat tccagccggt ggcaaagaaa ccaccgtagc gccagaggtt gtcgatactg
    14161 gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat cataccgatg
    14221 atcgccagca ctttaatcag cgcaaaccag aactcggttt caccatataa gcgcacgttc
    14281 accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca cgttggaaca
    14341 tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat tcccaccagc
    14401 acgaacatta cccagtagtt ccagccagag aggaagcccg caaacggtcc ccagtattta
    14461 taggcaaagt gggcaaatga accggatacc ggctcctcaa ccaccatttc gccaagctgg
    14521 cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac agccggaccc
    14581 gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc gccacccagc
    14641 gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac
    14701 gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg
    14761 ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc acacggttaa
    14821 acggggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc agcttatacg
    14881 ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca atcatcggcg
    14941 cggcaatccg gctcatcacc tctgaaccag cccccgttcc ccacagaatc ggcagcagac
    15001 cggcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcaggacc gcgccgtgat
    15061 ataacgcctc atccagcttc tgctcgctga atgtttgtgg attattcaac gacggcacgg
    15121 cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg gcgaccccgg
    15181 cgagggcgat aaagccagtg cccgtcgcca cggaaagatg aaagcccatc caccacagca
    15241 accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac gcttcgccca
    15301 cccgacggaa cgccagatac aacagcacga agataatcat caacgtcatc ggcaccatga
    15361 gtttaagctt atggttggcg cgctccagta gctcgaactg cccggagaat gccacgctgg
    15421 tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga accaccgaca
    15481 ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca ttctcggttt
    15541 tcagcatcga cggtccggta gagactttaa tgtcggccac gtctgccagg gtgatttgct
    15601 gcttcatcgg cgtcaggatc ggcagctggc gcagtgcctg cggactatcg cgccagcttt
    15661 gcggataacg cagattaatt ggataacggg caatcccttc caccgtttcg ccaaccatcg
    15721 ccccgcccac cgcagaagtc acaaacaact gcacatccgc caccgtcata ccgtaacgtg
    15781 cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc cgctcggcaa
    15841 gcgcagaagc tacgcctggc accgttcgcg ctacttcttc aatttgctca gccatcgcgt
    15901 cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta atgccggttg
    15961 agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc cccggcagcc
    16021 gcacggtgtt atccagttcc tcaatgattt tgtccatcgt catgcctggc cgccactgct
    16081 cctgcggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa tcggtggcgg
    16141 tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc ataattagct
    16201 tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg
    16261 gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca actttattga
    16321 gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt ttcggccagt
    16381 gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg agcgggttac
    16441 tgctttccgg cggaattttg ccacggatcc agtagcccat caggatcggg atcactacga
    16501 tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac gggccaaaca
    16561 gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg ataatcagca
    16621 gactgataaa tagcgccggc cccacttcaa cagacgcatc ggtgatcacc tgccagcgcg
    16681 ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc cgtttatgcg
    16741 cattctcgat catgacgata gcagcatcga ccatcgcccc gacggcaatc gcaatgccac
    16801 ccaacgacat aatattggca ttcagtccct ggaagtgcat gacaataaaa gcaatacaca
    16861 accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag agaaacagcg
    16921 cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg ttgtcgatgg
    16981 cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct tccggcagac
    17041 tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg gcgtttttgc
    17101 cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttagt tcggcaatgc
    17161 cccggcgcat ctccgggcca atctggacct tcgcaacatc gcgcagataa acgggcacgc
    17221 cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc agatagccgc
    17281 tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc gcttcctggt
    17341 ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct
    17401 ggggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc acttccgcaa
    17461 cgtcagggat ggttttcagc tcatatttga gaaaccagtc ctgtaatgag cgtaaatcgg
    17521 ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg
    17581 tggcatctgg ccccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga
    17641 gatactccag cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat
    17701 acacataaga gtcgccaaac tgtgagaaac cgcgcacagt cttcgcgcca ggcaccgaca
    17761 acatggtggt ggttagcgga taagtcacct gattttcaac gatttgtggt gcctgaccgg
    17821 gatagctggt tttaataata acctgcacat cggagagatc cggcagcgca tccactggcg
    17881 tattaatgat ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa
    17941 aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc
    18001 atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc
    18061 aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc
    18121 ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc
    18181 atcaacggta atcacccgct gttcgctgcc ggtatcaatc agcgcttgtg acggaatgag
    18241 cagcatcggt tcgctggcgg tgttgagttg caaccaggcg ttcatgcccg gttttagcgc
    18301 ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc
    18361 aggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgcgccg gaacggtgag
    18421 ggtaaactgc gaggcatctt tcaccagcca ggcgatggac tccgggatcg cagcagtgac
    18481 ccacaccggg tccataccct gaattttcgc gaccacgtta tctttggcga tattcattcc
    18541 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
    18601 ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc ccgccagtcg
    18661 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa
    18721 atactcactc tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt
    18781 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
    18841 ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac tctgggcaaa
    18901 agtcagcggt ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
    18961 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
    19021 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca ttgggtcgta
    19081 ccagaataag attttacgtt ctgcggtcga cgttttttct gcaggcggtt ccgcctttgc
    19141 aacccaggta aagcctgccg cagaaataat accgcccgcg atcatgctgc cgataataag
    19201 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
    19261 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact
    19321 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tcacagcagc
    19381 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaataccct ttacctcgcc
    19441 agtggcacta ataacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
    19501 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
    19561 catttattct ctcctggagt taaaagttat tgctgccaac cgccaccaag tgcggtatat
    19621 aaagaaattt cgttaacctg acgggcataa ttcagatcaa gtaaagtttg tcgggttgca
    19681 aataaagaac gctcggcatc cagcacttcc agataactta ctgcgccgtg ctgatataat
    19741 gcccgcgccc gttgcaaagt aatttgcagc gatgccagat aacgctgctg ggcgctaatt
    19801 tgatcgttca ggctttgacg taatgccagc gcatctgcca cttctttaaa ggcgttctgg
    19861 attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg
    19921 gcctggttgc gtccggcatt aaaaatgggg atctcaattt tgggtataaa attccacatc
    19981 ccgctgctgg cgttaaataa tgacgataga tcgctactgg cggtcgatat tccgctggtc
    20041 aggcttatag acggaaaaaa tgccgcgcgt gcagcgccaa tattggcatt agccgccatt
    20101 aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaatccc
    20161 gccggtaatt taacgctttg caggctgtcg ctgtttactg tctgcgcttg cggcagcttg
    20221 ccgtagcttc ccagcaacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
    20281 ttagcgatgt cgctgcgggt actttctatc accccgcgag cctgttccag cgccagaaca
    20341 ttactgctac ctgtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
    20401 agcgtttctt cggctatttg caattgcgca tacgccagtt gctgattgaa atagctttgc
    20461 gcgacattag aaaccagcag aatatgcacc gcgcgctgag cttcctcagt ggctaaataa
    20521 ttttgtcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag gtcaaagctg
    20581 gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat tccctttaag attaccgctc
    20641 cagctgccgc tgccctcgcc attgagctgt gggtagcggt cggcatcggt cagacgatat
    20701 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccgatt attcaccagc
    20761 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccgcgttc
    20821 tgatagttat ctgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggcacg
    20881 ggcattgccg gacgctgata atccggtgcc agtgaacaac cggttagcgc aagggccaca
    20941 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttatgccgc
    21001 caactttact cgccaggctc tgattttccg gtgacagaaa aatgacaaaa ttgtcatttt
    21061 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
    21121 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
    21181 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggttgaat ggctaccatc
    21241 tggcgatgac cggtgattat gatctgataa tcctcgatat tatgctgccg gacgtgaacg
    21301 gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg gatgccgatt ctgttgctta
    21361 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggcg gatgactatc
    21421 tggtgaagcc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
    21481 gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca
    21541 gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagag tttactctgc
    21601 tggagttttt cctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
    21661 tgtgggacat gaatttcgac agcgacacta acgccattga tgtagcggtg aagcggctgc
    21721 gcggcaaaat cgacaacgac tttgagccga agctaattca gaccgtgcgc ggcgtgggct
    21781 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
    21841 cgcctgacct tttttatcag cctggccacc atcgcggcgt ttttcgcctt tgcatggatc
    21901 atgatccact cagtaaaagt gcattttgcc gagcaggata ttaatgattt aaaagagatt
    21961 agcgccacgc ttgaacgggt acttaatcac cctgacgaaa cgcaagcccg acgcttaatg
    22021 acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc agatagccac
    22081 ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc acgtgatgcc
    22141 atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc gacgattatg
    22201 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat taacttgtcg
    22261 gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct ttcgatcgat
    22321 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtaatc
    22381 agcatcctga tcgtctttat cgtactgttg gcggtacata aaggtcacgc gccgatccgc
    22441 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg
    22501 cagaccgtgc ccattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
    22561 attgaggatg tctttacccg ccagtccaat ttctcagcgg atatcgctca cgaaattcgc
    22621 acgccgatta ccaatctcat cacgcaaacg gaaatcgccc tcagccagtc tcgcagccag
    22681 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
    22741 gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc cgaaaagaaa
    22801 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc gttagcggaa
    22861 gatcgcggcg tggagttgcg atttgttggc gacgaatgtc aggttgcggg cgatccgctg
    22921 atgctgcgtc gggcgttaag caacctgctt tctaatgccc tgcgttatac gccgcccgga
    22981 gaggcaattg tagtgcgctg ccagacggtc gatcaccagg tgcaagttac cgtcgaaaac
    23041 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctaccgcgtt
    23101 gacccttccc gccagcgaaa aggcgaaggt agcggcattg ggctggcgat agtgaaatcg
    23161 attgttgtcg cgcataaagg cacggttgca gtaacgtcag atgcgcgggg gacaaggttt
    23221 gtgattatgc tgccggagag agagtgatta ttaagttcaa attcagggcg tagtgcatca
    23281 cctcctggta ttgttgatca acaaattaga gaaaaactta ttaaacatat gaaatatcga
    23341 aggcttttat ttttcaaagt ttcacgagat gtttgtatat gaaacttagt taaatctgtg
    23401 tttct
//
LOCUS       DAPHJE010000071        22359 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPHJE010000071 DAPHJE010000000
VERSION     DAPHJE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.071"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..519
                     /locus_tag="RH222_002806"
     CDS             <1..519
                     /locus_tag="RH222_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3797797.1"
                     /translation="ILLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNV
                     VDRVIEGAYEVVGVFDRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTAD
                     ILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRAL
                     WVMAETLLESLR"
     gene            574..1059
                     /locus_tag="RH222_002807"
     CDS             574..1059
                     /locus_tag="RH222_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV3797798.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1075..1551
                     /locus_tag="RH222_002808"
     CDS             1075..1551
                     /locus_tag="RH222_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV3797799.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKAGRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1614..1835
                     /locus_tag="RH222_002809"
     CDS             1614..1835
                     /locus_tag="RH222_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV3797800.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1998..2372
                     /locus_tag="RH222_002810"
     CDS             1998..2372
                     /locus_tag="RH222_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV3797801.1"
                     /translation="MSDALSGTMLPDDNHDRPWWGLPCTVTPCFGVRLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLNQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPATATTS"
     gene            2419..2796
                     /locus_tag="RH222_002811"
     CDS             2419..2796
                     /locus_tag="RH222_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV3797802.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGTSSQLINSIDILRARRATG
                     LMTRDNYRTVNNIALGKHPGAKQ"
     gene            2793..3281
                     /locus_tag="RH222_002812"
     CDS             2793..3281
                     /locus_tag="RH222_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797803.1"
                     /translation="MKLALKLEADSINVQALNMGRIVVDIDGVNLAELINVACDNGYS
                     LRVVDESDRTSADCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLFVTLIRRYGVSLIHLDAGAECLPGLPTF
                     NW"
     gene            3293..3490
                     /locus_tag="RH222_002813"
     CDS             3293..3490
                     /locus_tag="RH222_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797804.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            3587..3874
                     /locus_tag="RH222_002814"
                     /pseudo
     CDS             3587..3874
                     /locus_tag="RH222_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3946..4950
                     /locus_tag="RH222_002815"
     CDS             3946..4950
                     /locus_tag="RH222_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HDV3797805.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            5232..5513
                     /locus_tag="RH222_002816"
                     /pseudo
     CDS             5232..5513
                     /locus_tag="RH222_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(5794..6330)
                     /gene="yghD"
                     /locus_tag="RH222_002817"
     CDS             complement(5794..6330)
                     /gene="yghD"
                     /locus_tag="RH222_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV3797806.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(6332..7510)
                     /gene="gspL"
                     /locus_tag="RH222_002818"
     CDS             complement(6332..7510)
                     /gene="gspL"
                     /locus_tag="RH222_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV3797807.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADFVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(7507..8484)
                     /gene="gspK"
                     /locus_tag="RH222_002819"
     CDS             complement(7507..8484)
                     /gene="gspK"
                     /locus_tag="RH222_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV3797808.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPFAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(8487..9086)
                     /gene="gspJ"
                     /locus_tag="RH222_002820"
     CDS             complement(8487..9086)
                     /gene="gspJ"
                     /locus_tag="RH222_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV3797809.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(9083..9454)
                     /gene="gspI"
                     /locus_tag="RH222_002821"
     CDS             complement(9083..9454)
                     /gene="gspI"
                     /locus_tag="RH222_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV3797810.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(9451..10014)
                     /gene="gspH"
                     /locus_tag="RH222_002822"
     CDS             complement(9451..10014)
                     /gene="gspH"
                     /locus_tag="RH222_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV3797811.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(10018..10473)
                     /gene="gspG"
                     /locus_tag="RH222_002823"
     CDS             complement(10018..10473)
                     /gene="gspG"
                     /locus_tag="RH222_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV3797812.1"
                     /translation="MYSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(10490..11713)
                     /gene="gspF"
                     /locus_tag="RH222_002824"
     CDS             complement(10490..11713)
                     /gene="gspF"
                     /locus_tag="RH222_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV3797813.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11713..13206)
                     /gene="gspE"
                     /locus_tag="RH222_002825"
     CDS             complement(11713..13206)
                     /gene="gspE"
                     /locus_tag="RH222_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV3797814.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(13206..15266)
                     /gene="gspD"
                     /locus_tag="RH222_002826"
     CDS             complement(13206..15266)
                     /gene="gspD"
                     /locus_tag="RH222_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV3797815.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(15296..16255)
                     /gene="gspC"
                     /locus_tag="RH222_002827"
     CDS             complement(15296..16255)
                     /gene="gspC"
                     /locus_tag="RH222_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV3797816.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(16273..16683)
                     /gene="gspS2"
                     /locus_tag="RH222_002828"
     CDS             complement(16273..16683)
                     /gene="gspS2"
                     /locus_tag="RH222_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDV3797817.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16749..17558)
                     /gene="pppA"
                     /locus_tag="RH222_002829"
     CDS             complement(16749..17558)
                     /gene="pppA"
                     /locus_tag="RH222_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDV3797818.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGVVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(17706..22271)
                     /gene="sslE"
                     /locus_tag="RH222_002830"
     CDS             complement(17706..22271)
                     /gene="sslE"
                     /locus_tag="RH222_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDV3797819.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRV
                     TGDITCNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDA
                     QELAGSDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFK
                     KLVNEEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIIL
                     SEGRLVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKS
                     TIALTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLS
                     NGATLDEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQ
                     IQGVINKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARND
                     KNYWLAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVI
                     GNPHYNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKA
                     SMTVGTNLDTVYFKRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNG
                     FEYVTQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESA
                     SGFVRLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTID
                     SKTGEVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKE
                     KIFAAFPGLKECTNPAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQ
                     AADLGTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKND
                     ELGFKTFTEFLNCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNY
                     MEKPLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQST
                     GLWAPAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTV
                     KFKVPYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAF
                     VYTTPKKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKNLTGHKH
                     RFTNDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPG
                     ATEVANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQL
                     KEWAEKNFDIKKWYPEGELPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYC
                     AESNGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNT
                     LASLKLPKPEQGPETINKVTEHKMSVE"
BASE COUNT         5100 a         6273 c         5626 g         5360 t
ORIGIN      
        1 attctgctca actctcatga cggtacctcc agctaccaga tgctgccggg gtacttcagg
       61 ttcgtctgcc agaacggatg cgtctgtggt cagtctctgg gggaagtgcg tgttccgcac
      121 cggggaaatg tggtggacag agtcattgaa ggggcttacg aggtggtggg ggtttttgac
      181 cggattgagg aaaagcgtga tgccatgcag tcgctgattc tgccgccacc ggcacgccag
      241 gcgctggcac aggcggcact gacttaccgt tatggtgacg aacatcagcc cgtcaccacc
      301 gccgacattc tgacgccacg acgccgggag gattacggta aggacctgtg gagtgcttat
      361 cagaccattc aggagaatat gctgaaaggc ggaatttcag gtcgcagcgc taaaggaaaa
      421 cgtatccata cccgtgccat tcacagcatt gacactgaca ttaagctcaa ccgcgcattg
      481 tgggtgatgg cggaaaccct gctggagagc ctgcgctgat tcagccatta ccctgaaaac
      541 cacgcattta tttgtccttt aaggagtcat ttcatgaaaa ccatttcaca taattccacc
      601 acatcttctg tttccgtaac cgctgcatca gggaatgacc agcctcagct ggttgccacc
      661 atagtccctg atgaacagcg catcagcttc tggccgcagc attttggcct cattccacag
      721 tgggtgaccc tggagccccg tgttttcggc tggatggacc gtctgtgcga agactactgc
      781 ggtggtatct ggaatctgta cactctgaac aacggcgggg catttatggc ccccgaaccg
      841 gatgacgatg atgacgaaac atgggtactg ttcaatgcca tgaacggcaa ccgcgctgaa
      901 atgagcccgg aagccgccgg cattgctgcc tgtctgatga cgtacagtca tcacgcctgt
      961 cgtacggagt gttatgccat gacggtccat tattaccggc tgcgggatta cgccctgcag
     1021 catccggaat gcagcgccat tatgcgcatc atcgactgaa tggaggcagg gacaatgcag
     1081 cagctttcct ttctgcccgg agaaatgacg cccggcgagc gcagtctcat tctgcgggct
     1141 ctgaaaaccc tggaccgcca tcttcatgaa cccggtgtgg ccttcacctc cacccgtgcg
     1201 gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagagtt ccgggtgctg
     1261 tatctgaata accagaatca gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac
     1321 cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgtaccacaa tgccgctgcc
     1381 gtggtgctgg cgcacaatca cccgtccggt gaagtcacac ccagtaaggc aggccggctt
     1441 atcaccgaac gtctggtaca ggcactgggc ctggtggata tccgggtgcc ggaccatctg
     1501 atagtcggtg gcaaccaggt tttctccttt gccgaacatg gtctgcttta acccgtcaca
     1561 accacatcac acctgttttc acttttatct tctgtcttca gaggtatccc attatgaaaa
     1621 ttatcacccg tggtgaagcc atgcgtattc accaacaaca tccgacatcc cgtcttttcc
     1681 cgttctgtac cggcaaatac cgctggcacg gcagcactga agcgtatacc ggtcgtgaag
     1741 tgcaggatat tcccggtgtg ctggccgtgt ttgctgaacg ccgtaaggac agttttggcc
     1801 cgtatgtccg gctgatgagc gtcaccctga actgatgttc cgtcacccgg aaaacgagta
     1861 cgactggctg agctcagacg ccgctgtgga tgaggccctg ctcataaacg aatacacctt
     1921 taccgaaggc ggtctgcgcg ccggctgacc tgtcatcgtt aaaccattct taaccgattt
     1981 tctgtgagga ttttatcgtg tcagacgcac tctccgggac aatgcttccc gacgacaatc
     2041 acgaccgccc ctggtgggga ctgccctgca ccgtgacgcc ctgtttcggg gtacgtctgg
     2101 tgcaggaggg taaccgcctg cattaccttg ccgaccgcgc cggtatcaga ggcctgttca
     2161 gcgatgcaga tgcgtaccac ctgaaccagg cctttccgct gctgatgaaa caactggaac
     2221 tcatgctcac cagcggtgaa ctgaatcccc gacatcagca taccgtcacg ctgtatgcaa
     2281 aagggctgac ctgcgaagcc gacaccctcg gctcctgtgg ttacgtttat ctgactgttt
     2341 atccgacacc ggcaaccgca accacctcat aaccccgaag ctctcacctt tagcactacc
     2401 agttacagag aacttaagat gaaaacatta tccgacacac atgtacggga ggtatctcgc
     2461 tgcccgtctc ccgtcaccat ctggcagaca ctgctcatcc gactgctgga ccagcattat
     2521 ggcctcacac tgaatgacac accgttttcc gatgaacgtg tgattgagca gcatattgag
     2581 gcaggtattt cactgtgtga tgcggtgaac tttctcgttg aaaaatacgc cctggtacgt
     2641 accgaccagc cgggattcag cgcaggaact tcgtcgcagt taatcaacag cattgatatt
     2701 ctccgggcac gccgggcaac cggcctgatg acccgcgata actacagaac ggtaaataac
     2761 attgccctgg gtaaacatcc gggggcgaaa caatgaaact ggccctgaaa ctggaagccg
     2821 acagcattaa cgtgcaggca ctgaacatgg ggcgcattgt cgttgacatt gatggtgtta
     2881 atctcgctga actgattaac gtggcctgcg acaatggcta ctcccttcgt gttgttgatg
     2941 aatctgaccg gacctcagca gactgcacgc caccatttgc tgccctgacc ggcatacgct
     3001 gcagtaccgc acatatcacg gcaaaggaca acgcctggct gtactcgctg tcgcatcaga
     3061 cgaatgacac cggcgaatca gaatggattc attttacagg cagcggatat ctgttacgta
     3121 ccgatgcgtg gtcatacccg gttctgcggc ttaagcgcct gggcctgtca aaaacgttcc
     3181 gccgtctgtt tgtcacactc atccgacgtt atggcgtcag tctcattcat ctggatgccg
     3241 gcgctgaatg cctgccgggt ttacccactt tcaactggta accaggagca acatgaaatc
     3301 attaaccacg gaaacagcgc tggatattct gattgcgtgg ctgcaggaca atatcgactg
     3361 cgaatcagga attatctttg acaacaatga ggataaaacg gattcagcag cactgttgcc
     3421 ctgtatcgaa caggccagag aggatatccg taccctgcgc caactgcagc ttcagcacca
     3481 gaaccggtga gtctcactca tcatctcact caccagactt cattccactg acgccagcct
     3541 gaacacggct ggcgttttca tttatctgca aaaaggaata tcgattatgt ctgaaatcac
     3601 agtctcccgt ccagaagtgg tcaacgagaa tacggacgtt atctgctcca cctcagtcag
     3661 gtccaggtca ctggaatatg ataattttcc ggaaatcagc gaagcgaaca ttctgagcac
     3721 atttgaacaa ctgcaccaga acaaagatga agtgtttgaa cggggagtga tcaacgtctt
     3781 caaagggctg agctgggatt acaaaaccaa ctcaccctgt aaatttggca gtaaaattat
     3841 cgtcaacaat ctggtgagat gggaccagtg gggataatga tatggtcccc cccaccttaa
     3901 cagcagtacg ctgttaaggt aaatcccttt aacggagaga ccatcatgca gaacatcaca
     3961 cttattggta tcgatctcgg aaagcactcc ttccacattc attgtcaaga caagtctggt
     4021 aaggctcttc tgcgtaaaaa atttacccgt actaaactga tgcagtttct ggcgacatgt
     4081 ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac actttatggc tcgctgtatc
     4141 tctgattttg ggcatgaaac taagctgatt tctccacagt tcgttcgccc gtttgtcaaa
     4201 agtaataaga atgattttgt tgatgcagaa gccatatgcg aagcggcttc acgaccatca
     4261 atgcgttttg tccagccaag aactgagacc cagcaggcca tgagggcgtt acaccgggtc
     4321 agagaatcgc tcatcagaga taaggtgaaa acaaccaacc agatccacgg tttcttgctg
     4381 gaatttggaa tcagcctgcc gataggtgat gctgtaataa aacggttgtc tctcgtgttg
     4441 gctgaacacg aaatccccga atatctgagc cgccttttgg tgaggttgca tacccactac
     4501 ttttaccttg ttgagcaaat agcagagctg gaatcagaat taagtcagtc aatcaacgct
     4561 gatgataccg ctcagcgcat tatgacaatt cctggtgttg gtccgattac tgccagctta
     4621 ctatcgtcgc aacttggtga cgggaaacag ttctcatgca gcagggattt tgcagcttcc
     4681 acaggacttg ttccccggca gtatagcact ggaggaaaat cgactctgtt gggtatcagt
     4741 aagcgaggtg ataaaaattt acgccggtta ctggttcagt gtgcccgctc attcataatg
     4801 cgacttgaac gcaagcatgg aaaacttgct gagtgggttc gggagcaact caacaagaag
     4861 cattcgaatg tggttacctg cgctctggca aataaactgg ctcgaattgc ctgggcaatc
     4921 actactcaga aaaaagaata tcaggcctga tatagatgct gatataactc aattagttta
     4981 ttttaaacaa taagatacaa gttacctttc tggttttgcg aagactgatt attgatgaca
     5041 tgaacggcaa atcgacctga ttaaaagcct aacagatgaa atggctcact gaagccgagg
     5101 ggatttttag gattatcagg tgcggaactc atcggggcgc gggcatcctt gcccacagag
     5161 acgccggata gatttaagca agccaaccat taatcaaaaa tcagtgttgc aaaaacgggg
     5221 gtgaccatag atttcatctt atcagtggaa tgcaggcaga tcgcctggct gacctggaaa
     5281 gaatgttgca tctgctcagc ggtaaaccga tccccgacaa ccgagggaat atcaccatta
     5341 atctggatga ccacatacag tccgttcagg gtaaaggacg ctatgaagat gagatgttca
     5401 tcattaaata ctttaagaag ggatctgcac acatcacttt caaaaggctg gagctgattg
     5461 acagaattaa cgatataata gccaggcact ttccttctgt gctctcagcc tgaccccgag
     5521 tttgattccc tttcgatatc aaaagggact gcgggtacaa aagagggtac atatttcacc
     5581 aaaccaaaca aaataaacta atatcaacat gatagaagca ttcttcgatt ccgagtccgg
     5641 caccactaat tcttaagaac ccgcccacaa ggcgggtttt tgcttttgga tctgacaata
     5701 accttcacga aaaaaattag cttataaagt ctgggggaat tactctcgcc acgttaacga
     5761 gagtaatttt attgatatta atctcctgat actttacccc cgtccaaact ccagccgctg
     5821 cacattcacc atcccaggct tctcagcagc actgacatca atttgtgtca cccgcagcgc
     5881 atatttttca tccagtgcgt ttaaccattt cagcaggtca ttaaacacca caggttctat
     5941 ccagacctga atattctccc cacgctcggc tatccgcctg atgactaccg agtgcgcgga
     6001 agcactgtca ctgataaccc gcgatacctg cgcaggcgtt gttgtggcgg attttcgcgc
     6061 cgcaataata tccggcgcgg cgctcttcag tcgggtgttc atcgccacca gctgctgcaa
     6121 tattgtctcc tgttgctcaa tccgttcgct caaaggctgc cagatgagga cgtaatatcc
     6181 gacgctaaac aggaacacca ccgctgccag taacatgccc ttttcacgcg gagaacgccc
     6241 cgccaggtgt tgtgccagcc agtgttcgcc acggcttaac tggcgttcac gccattgctg
     6301 aaaatagtga ataaatttat cgcgtaacat gttatttcct ccgcaacgtt acgccgccgg
     6361 aaaccgcatc accctctttc tgtaacgcgt cctgctgcac aacaaaatct gccgccagtg
     6421 cgctacgcag tttgtcgaag ctggcaaagt tcgcggcccg caactggagg tgaagcgtct
     6481 ggcgtttttg atcaaaggtg aaaccacgca tttcgatgtc ggaaagtgac gctgatttca
     6541 gggtgctggc tatcgctgac aactcggcga gcagccgggt atcgtcggcc tgtgggcgat
     6601 attttttcag cgccatcgtc acctgagagc gtaaattcac aatccgcttc tgctccggga
     6661 acaacgttaa gaactgttcc tccgcctggg tgcggctttg cgccacctgt tcgctgacgc
     6721 tccataacgt cacgccccgc tccactgcca gcgcaaccag aatcagcaat atcggcagaa
     6781 tcatcacccg ccagcgcgcc cactgttttc ggtagctgac gcgaggctgc cacggccctg
     6841 tcagcaggtt cccttccggt tcgccataag tggtaacggc gggcagagct gtaacggtca
     6901 ggcgttcagc gtctgcgcca gcccatgctg atagcttttc cggtgcaatg ccgactacgg
     6961 ttagcgaaag cggtaaatcc tgctcattga gctgggcgcg gaacatgacc ggagccagcg
     7021 ccagcccggc gctccatccc cggcattcat cgatgcggca gataacccgt tgcgcatcgc
     7081 cagccataaa cccacaagga atggacatcc agtccggcgc gacggtagcg cgggtgatgc
     7141 cgttttcctg caaccactgc gcaatgttgc gcatatgctg ttggtgaatc actgctacgg
     7201 ttgccagttg ctggtcgatt ttcaacgggg cgaaatgcag ttcatcgata tcctggttca
     7261 gctcttcttc cagtaaggcg ggcagaatag tcggtatctg cttgcgggcg acaacaggca
     7321 gttcaacctg ccagacgctg atccattcgc cgggaatgta gagtcgaatc gcatcagttt
     7381 gcagccattg ctggagctgt tcgttagcaa cgtcagacca gataccgtgc tccacatcgg
     7441 gtgtacggcg ctgccaatgg atgggatcag cggcgcaaag cgggaaaaaa atctcaagga
     7501 tggaactcac tcactttctc ctgtctgatg ccagagaacc gaaaagtgtt gtgggcccat
     7561 gcggacaatt aacgagttca tcgtcagttc aatctcattc acggtgatat ctgaacgcag
     7621 ccagaagtaa ttgctgtcca cgctcaggac ggtttttagc tgttttttag tacgctcatc
     7681 gacgtcagca agtagcggct gtgcaagaaa ctgatcgaca tcttcccagc ccttcgccgg
     7741 acgttgttgt aataacgccc gcgcctgaac agggcttaac cacgggtcaa acagcgcctc
     7801 aagaatcaca ctttgcgtga cgtctaaagt attgatgttg atttgctggc gggtcatcgg
     7861 cagcgcacag accagcggtt tcagtttttg ataaagcccg gcgtccattc cctgcaccac
     7921 gcgcatctcg ctgatatcgg ccagcggttg attggcggcg tagaaaggca ccgagcgggc
     7981 gagatattcg ctgtcttcac ggcccagacg cgtctgcacg ctgcggtctt catcaataaa
     8041 ctcccacagg ctttcggcta tcagttcggc ccgataagca ggcacatcca ggcgcgtgat
     8101 cagggcaatc agttgttgta ccgcgaacgg acgcgacgcc gttgtgggct gagcgagggc
     8161 attcaggtta aagcaggcct gtgcgtcacg cagagtgacg gcgatttgcc cctgcggcag
     8221 tgggaaaaaa cgagggccgg aagcccacgg ttgtgccaga tgcacgcgct tttcattttt
     8281 caggctcaga ctgagcgcgc tcaacgccag gctttctgca ctggcgctgt accacagcgc
     8341 ctgctggtac tcctgctggc tgcgcgttct tcccagttgc tgctgcatcc gcccggaaag
     8401 cgtgatggtt accagcatca taactgccag caataccagc accacaacca gtgccattcc
     8461 gcgttttggt ggtgaggtga tcatgattat tgcggcccgc gtaacaacca gatgcgttca
     8521 atctcgcccc attgcggcga atgcagggtc atgcgtaccg ccacagggat cgcctgtact
     8581 gacgaccagc tctcctgcca gcgcgtgccg tcgtagaact gcaaacggag cgaatcggcc
     8641 ggaatcaact tttgcattgt cggcttcacg ctgcctgcgg catcggtcag cggccaggct
     8701 aaccgttcga gataaccgtc atgaatgcgg taaccgacgg tgagcagatt actgcgcggc
     8761 agacgcatca acgggttaac cacaccgccg cgcacaaaac gcattccttc actctcagac
     8821 gccagcacgc cagcgccagc cagtaacgct ggttcacgct ggccctgatc gcctcttacc
     8881 ggacgcggca tcatttgggt cagatcgtgg gtcagaaaac tcatcgtttg ctgcatgagg
     8941 tttagttttt gatcgtgtcc ggcgacggcg ctattcacgc gcgtgacgcc gttagtcacc
     9001 tgctgcgcca tcagtgccag cgaggcaaaa atggcaattg ccaccaacat ttccagtaac
     9061 gtgaaacctg cgcgagtcct tctcactgtt ggcctcccac ggcgctaaac caggcgcgtc
     9121 gtgactgaat caccgacgaa aagtcttcgt gaaggctgac ttcaatatcc accgcctgga
     9181 gcagcgcgtt gcctgtactc agcggtgtgg ttcgccagta ccagcggctt cctgccatat
     9241 cgctctcgcc ctgctgcctg accgcccgct gctcgcgggt catcaggtag agcagcgcgg
     9301 tctggttttc agccacccag cccgctaccg ttttttcctc aagaatttgc tgattactca
     9361 gcgcaccgct ggcaatctgt aacaccgccg tggcagccag cgcaaaaatc gccagcgcga
     9421 gcatcacttc cagcaaggta aatccgcgct tcatggcatc ctctcatcac attgattgag
     9481 ggataacgcg ccatcgtgcg ccagttttac cgcccaacat gcgttttgcg ccgccgagta
     9541 gaagcgcagc gtaaacggcg tggcgggttc aaaaggcgaa aaacggatct gcggcgtctt
     9601 ctttttcgcc tctttttgca gctccagttc aatatcgtgc agcgtcaggc gacgacgttg
     9661 cagctccagc gcatactcct tctgccagat atcacttccg ggttgtaaca gcatctgcac
     9721 ctgttttggc accgtaacct gtgccgataa ccgggtcgcg gaaacgggca accactgccc
     9781 ctgacgacgc tgcataaact gatagccagg cgcgtcgatt agcacaccga gtgtctgccc
     9841 ttcaatcact gccctgtcct taaactgcgc aaagcgagtc agaaaatcct gtgccgcttt
     9901 tttcgcgggc ggttcagaat cggtcgcaaa cgtctgtacc acgcccgaac tggcaaggcc
     9961 gataaggaaa atcaccaaca tgatttccag aagtgtgaat ccgcgttcag gcactgctta
    10021 ctgaaactcc tgcaaattcc agttaccgat atctgcgcca gcgccttccc cgttttcctg
    10081 accatccgcc cccagggtat aaacatcaaa cagtcctttt tcacccgggc tgagatactg
    10141 ataatcattg ccccacggat cctttggcag tcgtttgatg tatccaccag tacggtagtt
    10201 gcgggagtcc gccatattgg ctggctgctg gatcagcgcc tcaagcccct gttctgtggt
    10261 cggataacgc ccgttatcca gtcggtacat atccagcgca ttctccagcg ccacgatatc
    10321 gctgatggct ttttgccgat ccgctttctc tttattgccc aacaggttag gcaccaccag
    10381 acttgccagg acgccaagaa taacaatcac caccatcact tccagcaggg taaaacctgc
    10441 ccgtggtttt tgtgtgcggg ataacgaata catgtgataa ctccgtaaat tacattccaa
    10501 ccatattgtt cagttgcagc atcggctcaa ggatggcgat gacgataaac agcaccacgc
    10561 ccgccatcat caccaccagc gccggttcaa acagccctaa cgccagcccc acctgggtat
    10621 caaattcccg ttcctggttg actgcggcct gctcaagcat ggtttccagt tcgccgctct
    10681 gttcgccgga ggcgatcatg tacagcatca tcggcgggaa caggcgcaac tccgccagcg
    10741 cggcgcgcag actgcttcct tcgcggacgc gatctgccgc cagcagtagt tgttgttcga
    10801 catagcgatt tgccgacacg gcggcagcgg tctgaatacc ttccagtagc ggaacactgc
    10861 tggcggtgag gatgcttaag gtgcgggaaa aacgcgccgt attcagcccg cgcgcaacac
    10921 gccccgtcac aggcaggcgc agcacggttt gatcccagcg caggcgcata gcaggatttt
    10981 taagtagccg ttgccccagc accagaagcc ccagcaaacc cgccagccag taaacgccgc
    11041 tggcctgtaa cgcgtcgctc atggcaataa gcgtgcgggt ggaggcgggc agcgcgtgtc
    11101 cgagatgatc aaactgttcg atgattttcg gcaccactgc cgtcagcaaa atagtgacta
    11161 cgcccgttgc caccaccagc agaaccagcg gatagagcat ggcctgcaac agacgtgatt
    11221 tcaggcgctg tcgctgttcg gtgtaatctg ccaggcgatt gagcaccacg tcgagatgcc
    11281 cggatttttc tccggcagca accatcgaac aaaacaggga atcaaagacg cggggatgtt
    11341 cgcgcaggct gtccgacagg gtgtaacctt cctgaatccg gctgcgcagc gccattccga
    11401 ggctttttac atgcagtttt tcactttgct cacttaccgc ctgtaagcag gtttccagcg
    11461 gcattgctgc ctgtactaac gttgccagtt ggcgcgtgaa cagcgccaga tctgccgccg
    11521 ccacgcgacg atgtgcgtgc cgccgacgct gcaacatccc ccctgacgaa gtattcatcc
    11581 gggcttcaat atgcacgggg ataagctctt taccgcgcaa caactggcgg gcatgacgcg
    11641 cggaatccgc ctcaatcata cctttggttt tgcgaccatt acgctccagc gcctgatagt
    11701 aaaacagtgc cattacgcct ccatggttac ccgcagaact tcatcgagag aggtttctcc
    11761 ggcgagcact ttctcaatgc cgttgctgcg gatacccgca gagtgttgtc ggacataacg
    11821 ttccagctcc agctccccgg cctgacggtg gatcaaatca cgcaatgtgg catccaccac
    11881 gatcagctca tggatggcag tccgtccgcg aaaacctttg tgattacagg cgggacagcc
    11941 ctgtggatgg tacagagtga cggtacgggc gtcggtaatt cccagcaggc gtttttcttc
    12001 gtcagtggct ggtgcgggct ggcggcagtc gggacacagc gtgcgaacca gtcgctgcgc
    12061 catcacgccc gtcagactgg aagagagcag gaaaggctcc acgcccatat cctgcaaacg
    12121 tgtgatcgcc cccaccgctg tgttggtatg cagcgtggaa aggaccaggt gtccggtaag
    12181 tgaagcctgg acggcgattt ctgcggtttc ggtatcgcgg atttcaccga ccatcaccac
    12241 atccgggtcc tgacgcaaaa tcgcgcgcag gccacgggcg aaggtcatgc cgacgcgggt
    12301 gttaacctgc gtctgaccga tcccttcaat catgtattcg atgggatctt caaccgtgag
    12361 aatgttgcgc gaatggttgt tcagctcctg caatccggcg tacagcgtgg tgcttttgcc
    12421 ggaacccgtc ggccccgtca ccagaaagat gccgtgcggt ttgtgtaaca gctggcgcaa
    12481 ctgcgcggtc agttgctggc taagccccag acgctccagc gtcaggcggg cctggttttt
    12541 gtccagcagt cgcagcacca cgcgctcgcc ccaggcagaa ggcatggtgg agacacgcac
    12601 gtcaatcgcc cggccgccca gcaacagcgc aatacgtcca tcctgcggca cgcgcttttc
    12661 ggcaatgtcc agccgcgcca tcaccttgat acgcgacacc agcagcgagg ccagtttgcg
    12721 ccccggacgc agcatttcat gtaatgtgcc gtcaacacga aaacggatca ccagactctt
    12781 ttcaaacgtc tcaatgtgga tatccgaagc gccttctttg attgcctctg ccagcatggc
    12841 gttgatcagt ttgatgatcg gcgcatcgtc gtcgctttcc agcagatctt ccgtttctgg
    12901 cagttcttcc gcgagagtaa aaaagtcctc agcagaaccg agatcttcca tcagctggcg
    12961 agcctcggaa gagtcccgct gccagaccgc attcagccgc tgttcaaatt cggcctcgtc
    13021 gatttgccgc agcgtaaagg gcgcgttcag cccccgctgc agctcctgca aaacagagag
    13081 cgatagcgga tgaacgtgga ggatctccag cgacgcttcg caccatgcca ccaggctaaa
    13141 ccgacggctg aaactgtagg gcagacgcac ggtgttggcg gtggtttcct gtgctacagg
    13201 caccattaac gcgttctccc ggcattgagg aacgcgcgaa cttccggcgg taaggcctgg
    13261 ttttgcgctg gcagtaccgg ctgtgcggtg tgcggcatca ggcttaaacc ttgctcatca
    13321 cgatagatct gttcggcgcg catatagtta tatttgcgct gcgacacgcc gtctgccgcc
    13381 ataccgtcac gcagaatggt cgggcggata aacaccatca ggttacgttt ttctttttta
    13441 tccgccgtcg atttaaacag gttaccaatc aatgggatat cgcccagcag cggcactttc
    13501 gccacgcttt ctcccgcctg atcgtccatc agaccaccaa gcacaatcag ctcaccatcg
    13561 ttagccagca cggtggtttt cagtttgcgc tcaccaaaca ccacgtcgag gctggtctgt
    13621 ccttccacct tcgagacttc ctgctcaatc accatctgta ccgcgttacc ttcgttaatc
    13681 tgcggcgtga ctttcagcat gatgccgact tttttccttt ctactgtatt gaaaggattg
    13741 ctgttattgg agccaacggt agagccggtt aataccggaa cgtcctggcc caccatgaag
    13801 aaggcttcct ggttgtccag cgtggtgatg ctcggcgtgg agagcacgtt cgagctggag
    13861 tcgtttttaa ccgcctgcac cagcgccatc cagtcgcctt tcaccacacc aaccgccgta
    13921 ccgctaaagc cggaaagaag ctgggcgagc gtggagagat cgccgttggt atccggatta
    13981 atggtggtag cgccgttttc actgatcacc gtcgagcctt tctgcggttt cgcctgagaa
    14041 atggctgccc ccagcgtacc gatagggatc tgcgtaccat tagcaaactg cattaatccg
    14101 gcatctttcg acgcccactg cacgccgaag ttgatattgc taccttcggc aacttccacg
    14161 atcaacgcct cgacgtgcac ctgagcacgg cgaatatcca gttgttcaat cacgctttgc
    14221 agcgactgca tgatgtcctg cggtgcggtt acaatcaggg cattactgtg tttgctggcg
    14281 gcgatggata caatctcacg cccgctaccg accgtgcctt ccgcctcttc tttagccgcc
    14341 gtgagcgtgc cgctgacctg cttcagcaca tcgaccagat cttcggcttt gctgtatttg
    14401 agatagaaaa cctggctgtt gccgctgcgt tccatttctg agtccagccg acggatcagg
    14461 cggcgcattt tgtcccgcgt ggccgggtca ccactgacaa tcacactgtt ggtgcgttcg
    14521 tcggcgacaa tttgagattt cagcgtcgct ggctggttct cgccgctgtt tttcgtcagg
    14581 ctttccagca cgcgggcgat ttccgaagcc gaggcgttat ccagcgggat cacctcttcg
    14641 gtgcgattac ccgcgtgatc cacgcgctgg atcacttctg tcaggcgctc cacaacggag
    14701 gcgcgtccgg tgagcataat cacgttggag ggatcgtaat taacaacgtt gcctgagcct
    14761 gcgctgtcga tcatctggcg cagaatcggt gccaactcgc gtaccgacac attacgtacc
    14821 ggcactactt tggtgaccat ttcatcgccc gcgtagttgt cgctgccttc accgaccagc
    14881 ggcagcggct cgactttcgc agcgctggat ttcaccacct tcagcacgtc gttttccatc
    14941 ggtacgacgg catacccctg tgcttccagc aggttaagga atagctggta atactggcgt
    15001 tcattgagcg gggtcatggt gcgaatactc actttcccct gtacgcccgg ccccataatg
    15061 atggttttat taaggttagc gccgacggtt tcgatgaacg atttcaggtc ggtatcttta
    15121 aaattagcgg tgaaagtggc ttcttccgcc cagaccggtg aactgcataa tgccgctgcc
    15181 agcaccagcg gcagtaaacg cttttttgtt ttgaggccag ttgtcttctt acgccagacc
    15241 gacaacgtaa tatcacgcca aaacacgatg aatgattctc ctggattaaa tgcggttagc
    15301 gcagcgcgat ggaaatgtcg tggcgcgcac ccttgcgtaa aaccgtaagt tgaatggaat
    15361 ccattgaagg taactgccgc atcagagcaa tcattgctcg tggatcagtg aaatcctgct
    15421 gatttagcgc aatggcgata tcgccttcct tgaaaccgct ggcatcgaac agagaacgat
    15481 ctgcccccgg cttcactgca taaccgacaa ttccctcctt acgcacaggc gtaagctgga
    15541 tatagttaaa aattttctgc ggatctttcg ccagtgcctg acgcacggca gtcgggatct
    15601 ccactggcgc actgacagca ggttcagcaa cagcttgctt tgcttcgtca ctgacagctt
    15661 ttttgttggt cacggctacg gtggaatgcc cctcttctgc caggctcagg cgctctattt
    15721 ttccctgata gcgcagcatc acatggtcgc gattgatttc ctcaatcacc gcattgtgcg
    15781 agccaagcgt ttcaccctgc aaatagacct gctgtttacc gccttcttca ataaccgcgc
    15841 cgggtctggc accaaaggca atcccgcgca gcaccacatt aagacgcgtt tctgccacag
    15901 gcacaggttc gggttgtttt accggcgtgg cgacgggctg atatttgcca aaccagtttt
    15961 gctggctgat taattgcacg tcatttttat cgaacgtttt tgcatccgca cggggcggtt
    16021 tattcgccga tggggaaaca gccgtatatt ccgcagaaaa ggagaaatag cgccagagtg
    16081 aatgcgccac ttttgcagaa ataataagca gcatcagcca gaacatcccg cgtacaattt
    16141 ttcgcagatg ctctttgtct gtattaaaag attgtctctg gttaagagtg tgacgaattt
    16201 ttgtcagcca ttgaatgaga taaattcgtg cgtcacgaaa aacaacccgc gccaatttat
    16261 atctacccga cgttatgctt tgactattcc acaggtggta cgatccagtt tccgctgata
    16321 ctggttacct gtacgtgtat cattaatcgt tatgctgtaa ttaattccct cggtaatcat
    16381 taatttcacc gttgggtcag cgcacatttg tcgttgatag ctggttaaaa aggcgtcagg
    16441 cgtctgcgtg gtctgagtcc ccgcttcgct gataatggtc atttttaccg tcgtgccgct
    16501 actttgtgcc agcactaagg tatatcccgc agatttaatc ggcaggtttt ggctgatatt
    16561 ttgcgcctgt tttttcgcca gtaaactggc attttcatta tggctggcac agccacttaa
    16621 taatcctgta acgctcaaca atagcgatat taataccctc cctggcattt gttttatcga
    16681 catggtaaat aatctctaag gttattaata agagttaaaa tgtcactttg ataatgacgt
    16741 tgttatcatt aaaacaatgc ctgtagataa attgttgcta taccgcccag acttaaacac
    16801 ggtccaaaag gcagtgtggt tgatcctctt tttgtaataa cggcatatat caggccgcag
    16861 catgatgcga ttaaagcaac attgggtagc gacaacggcc ccacccaact acctaacgca
    16921 gcgaacaata atacatcgcc catgcctaat gcttctttac gcagaactac tccggctatc
    16981 cagcgcaggg agtaaaaagc gataaacccc accaggacgc cggtaactgc atcttgtagc
    17041 gtgagcgggc tctgctgcgc ccatgccgca atcagtcccg tccacaatac gccctgagta
    17101 aaaacatcgg gcagccattg gtgatcgagg tcaatgacgc tcgcggcaat cagccaggcg
    17161 gataatatca tcaccgccag cgcccatcca ctttccggcc agaccagact cgccagcaaa
    17221 aaagcgagtg ccgtcaataa ctccaccagc ggataacgct tgctgatttt cgcctgacaa
    17281 tcacggcagc gccctttgag catcagccag gagagcagcg gaatattgtc acgtatccgg
    17341 atggtctgct gacaatgtgg gcagtgggaa cgcggtagcg caaggcttat ttttgactgc
    17401 gcactcggca tttcaccatg aaactccgcc atttgttggc gcagcatgat ggggtaacgc
    17461 caaatcacca cattcaaaaa actgccgatg atcaatcctc cgacggttgc cagtatgggc
    17521 atcgccgcgg ggtattgctg aaaaacatca aaaagcatgg ttaaaggttg tttgttgtaa
    17581 cttgccggat gcgggcctgc aagtgtatgc catacggctt tcctccaggc ccgataagct
    17641 ggcgcaaccc ggtgcgcctt atttcatgcc ggatgcggcg tgaacgcctt atccggcata
    17701 caggattact cgacagacat cttatgctcg gtaaccttgt taatggtttc cggtccctgt
    17761 tccggtttcg gcagcttgag tgacgccagc gtgttgtacg ccgactggct cacaccgccc
    17821 tcgaagctca tctcgctcgc ccccggcagc tggtaagcat tcgcacccgg attccatttc
    17881 ttaaagaact ccgaaagatc cgtctgggcg acccaggagg cacacagcat cagcgtgtcc
    17941 gcagcgttac cgttggattc agcacagtaa ttcttgccac caaaggtctt gtcaccaacg
    18001 tcatcgccgc gcgctttacg gtgcatcaac tggaacaggt tccagccttt catcccttca
    18061 cgatcgctga agaacttcgg caattcacct tctggatacc atttcttgat atcaaagttt
    18121 ttctctgccc attccttcag ctgcgcgtac atcagcagac ggtcacccgc accgccgcgc
    18181 gcccatgcct gaccgttgct ctcctccaga tattccggtg cgacggtaat atcgtcagcg
    18241 acacggttca tcttgccgag ataacgatcc tgcatgtaca gcgccagcac gttgttcgcc
    18301 acttcggtcg cgcctggaac agtcagcggc gtttctgcgg cgttgtgccc tacttcgtgc
    18361 cagatcagcc agtcgttcag cggcgtcgtc ggcagcgtgg tgctgttcgt cgagaagctg
    18421 ctgttcatta ccggataacc cgagtgcgca tcaccgatgg agatctgcac atcgtttgtg
    18481 aaacgatgtt tatgtcccgt caagttttta taggtaaaca tccggtgctt accgctcgtt
    18541 tcatcacgac cgtagaagtc attcatcgag ctggcaaagg tgtccagatc gttagcgaat
    18601 tgctccagtc cgccagtgta attgctggca ttcaggttct tcttcggtgt ggtatagacg
    18661 aaagcgtctg attccagctc acccagcgga gccggtgagt tcagatcgtt tttccatgcg
    18721 ccgtctttat agaacggtgc ttttaccacg ccagtaaagg tgaagctggc agattcattg
    18781 gtagagctat tgcccttgat ataaatcagg ccaccgtaag gcaccttgaa cttcaccgta
    18841 ccgctagcgt ccagagagta cgttttagtc actcttggcg gacggttcag cgcaacttca
    18901 tgcttctcac gtccggtcag gtcgtcagcc agcgccacgg tgacggtcac aggaacgttc
    18961 gcattggact taatggtgac ctctttctga gccggtgccc acaggccagt tgactgcatg
    19021 ttacctgcaa accatttggt cggattcgag tacaggctga tggtttcagt aacgttctgt
    19081 ccctcttccg atactgctcc cggatacttc tcgacatcaa ctttgatgtt cagatcccac
    19141 caggaacggc ccagcatcag gcgtgtcagc ggtttttcca tatagttgag cgggtagctc
    19201 gggttcatca tgcccgcttt gctgctaccg tcaccgtaga tcatgttgtt atcgaccagc
    19261 gattttttca gatctgcaga acacttggtg ccgcctgcat aggcatcatt ggcgtagcag
    19321 ttcaggaact cggtgaacgt tttaaagccc agctcgtcat ttttgccttc ttcataacga
    19381 tagctcgtat cgttccacag ccagaccgac atgttctggt acagacgttc cagatcgacg
    19441 ctgctcagac gctcaccttt gcgaccattg gtccggaagt agagctcatg ctgatacaga
    19501 cgctgaatgt tggtgcctaa atccgcagcc tgcaccatcg cttttgcagt gtcggcgtta
    19561 aggcttagtt gcgtatactg tggaacatac atgccaccag taaccggaac ccccgtgcca
    19621 ggacgatatt ccaggcagtt gacctcatag tgatatgccg gattagtaca ctctttcagc
    19681 cccgggaacg cggcgaaaat tttctccttc gcagccttca gagaatcctc tgttttatga
    19741 tcggcctcat caataaaggc ataacgcgtt tcctgtttgc catctacatc ttccagccag
    19801 ctggcaactt ccagcttcgg tttgtcatca ggtttgtttt ctacctgata tttccactta
    19861 acttcccctg tcttactatc gatggtgtac ggcagcgcac catctacggc aggataacgt
    19921 tcatagaccc aaatgcccgt tgcgcgctgc tgacgaacgc ggttcggata cccttgcgga
    19981 tcgttattta ctaccgactt gttcagtgcc atcgacagac ctgcggcatc caacagacgc
    20041 acaaaaccag acgcgctctc ttccttaaga ttgctcatca cgttttccat gatcagcacc
    20101 gatccacctt tgttcagata ggcgatcaga tcggtcacat cctgctgagt cagcttcggt
    20161 ttgctggtat ctgcacgcag cgggattgca taaggatcgt tacccacctg agtcacatat
    20221 tcaaagccgt taaggatcag cagcggcatt tcctgcggat cgagatcgcc atagctactt
    20281 aaatgctcaa cagagatgcc cgcaaaatcc ggatggaagt cgaacgcagc gctgtttcct
    20341 gtaacctgac catgacgttt aaaatagaca gtatccaggt tggtgcctac ggtcatgctg
    20401 gctttcgcgt ccggcgtcca tttatcgtcg gacagatagc gcagcacgtt ctccatgaag
    20461 ttcttcatgt catccgggtc gctgttgagc gtacactgcc catctttatt aacgcccccg
    20521 ttccagctgt aaccgttcgg gcaacgcaaa atgctgttgt agtgtgggtt accgataacc
    20581 atcagtttgc cctcaccgac ttgccccagc gaaataaacg gcaggttgaa agtggcggta
    20641 tcgcgcgtaa cgttttccgg ctcaacaaga gaaggcgctt ccgtaatgta cgccagctca
    20701 tttttatccc aggcgcgttt ttcgccaaac gccagccagt agtttttatc attacgcgcc
    20761 atcagaatcg ggaaggccgc gttggagata ttcaccaccg cctgaccgcg cgcattaccc
    20821 gtgctgccat agaagttggt agagtcatgg aatacatgga acttactgac agatttgtag
    20881 ttcgtatcca cgccccacag cttgttgata acgccctgaa tctggccgtc attaacattg
    20941 cgcgttgtca gcgagaacca gcgagcctcg ttacaaccgt cggttttcgc acaaatcgcg
    21001 gtatcgatct ctttggcctg acccgtctta aactgctcga taaattcgtt tggcagatta
    21061 acaacttgct caccttcatc cagcgtcgca ccgttggata acgagagatt gataatctcg
    21121 ttgatcacgt tgggatattc ggcaaagacc ttgcgtacat catccggaac aacacgggta
    21181 ttattttgcc cggtcgtcga atagcgatga ataagctgat cgatattcgc cccgcgaact
    21241 tcatcaccca gttcagtcag cgcaatggtc gacttattgc cgcgcactga acccagttca
    21301 aaggtatcga taccaaagga gatggtttcg ccccagctaa aagaaaattc accattttcc
    21361 cctgtcacgc cacggcctga attggtgtag tagttgacgc cagcaacacc atatccctgg
    21421 ctatccacca ggcgaccttc tgagagaatg atttccgaag gttgatactg ataaaactgt
    21481 tccgcgttag ccgacacgaa ggaggcgttc agatccggtt ttgttcccgg agtcgtgacg
    21541 gggactaccg gtgaagtatg agtggatggc gctttatcag tcgcggcatt gttttccacc
    21601 tcttcattga ccagcttttt gaactcttcc ggtgccagat cgatttgctt atacagcgag
    21661 tcgaaacgtt tactctcgat caccgaggag aactccaggc acacctgttc tgtattcgcc
    21721 gggcaactgt tcatggaggt gaccagcgag agcgcattgc ttttcttgtt gtcggaaccc
    21781 gccagttctt gcgcgtcctc aagactaaac gacacttttt caaccgcacg caggctacgc
    21841 gcagcttctg actgggtgtc gaaggtagca attgtcgtgt tgttccctgc cacacaggtg
    21901 actttgtcgc ctggtgtaaa ggtaaagcca tcgctggatt catcattaca ggtgatatca
    21961 ccagttaccc gcaggcttcc gcccagggtc agataacccg ttttcgtagg aacaggttct
    22021 ggctccggtt ctggtgttgg ctcaggatca ggtgtcggtt ccggcgtagg ttctgggtcc
    22081 ggcgttggct caggcgtcgg ctccgggttt ggtgttggat caggtttcac ttccggcaaa
    22141 gaccctgttc cagaatctac aggcggcgta tcggaggaag atccggagcc accgccatca
    22201 caaccggcta acagggttgc actcaaaata gccgctaaaa gcgatttctt atatttaaat
    22261 ttcttattca ttaataacgc aagtgacaaa acaatgttaa aaaattcgta actgggagaa
    22321 atagttttat gctttatctt ctttaataat ttcctccat
//
LOCUS       DAPHJE010000072        22128 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPHJE010000072 DAPHJE010000000
VERSION     DAPHJE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.072"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..848
                     /locus_tag="RH222_002831"
     CDS             <1..848
                     /locus_tag="RH222_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV3797820.1"
                     /translation="FVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIFN
                     NTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIKVGY
                     GLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQYPTNPSESES
                     ASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDKIFS
                     IAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSGGGGSSGGGG
                     ASGRW"
     gene            complement(908..2206)
                     /gene="eno"
                     /locus_tag="RH222_002832"
     CDS             complement(908..2206)
                     /gene="eno"
                     /locus_tag="RH222_002832"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV3797821.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2294..3931)
                     /gene="pyrG"
                     /locus_tag="RH222_002833"
     CDS             complement(2294..3931)
                     /gene="pyrG"
                     /locus_tag="RH222_002833"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV3797822.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4159..4950)
                     /gene="mazG"
                     /locus_tag="RH222_002834"
     CDS             complement(4159..4950)
                     /gene="mazG"
                     /locus_tag="RH222_002834"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV3797823.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5021..5356)
                     /gene="mazF"
                     /locus_tag="RH222_002835"
     CDS             complement(5021..5356)
                     /gene="mazF"
                     /locus_tag="RH222_002835"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDV3797824.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5356..5604)
                     /gene="mazE"
                     /locus_tag="RH222_002836"
     CDS             complement(5356..5604)
                     /gene="mazE"
                     /locus_tag="RH222_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV3797825.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5682..7916)
                     /locus_tag="RH222_002837"
     CDS             complement(5682..7916)
                     /locus_tag="RH222_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV3797826.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7964..9265)
                     /gene="rlmD"
                     /locus_tag="RH222_002838"
     CDS             complement(7964..9265)
                     /gene="rlmD"
                     /locus_tag="RH222_002838"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV3797827.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9322..12078
                     /gene="barA"
                     /locus_tag="RH222_002839"
     CDS             9322..12078
                     /gene="barA"
                     /locus_tag="RH222_002839"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV3797828.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12310..13650)
                     /gene="gudD"
                     /locus_tag="RH222_002840"
     CDS             complement(12310..13650)
                     /gene="gudD"
                     /locus_tag="RH222_002840"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV3797829.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13671..15011)
                     /locus_tag="RH222_002841"
     CDS             complement(13671..15011)
                     /locus_tag="RH222_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV3797830.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15013..16365)
                     /gene="gudP"
                     /locus_tag="RH222_002842"
     CDS             complement(15013..16365)
                     /gene="gudP"
                     /locus_tag="RH222_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV3797831.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16800..17249)
                     /locus_tag="RH222_002843"
     CDS             complement(16800..17249)
                     /locus_tag="RH222_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV3797832.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17267..18049)
                     /gene="truC"
                     /locus_tag="RH222_002844"
     CDS             complement(17267..18049)
                     /gene="truC"
                     /locus_tag="RH222_002844"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV3797833.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18049..18378)
                     /locus_tag="RH222_002845"
     CDS             complement(18049..18378)
                     /locus_tag="RH222_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV3797834.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19000..19545)
                     /gene="syd"
                     /locus_tag="RH222_002846"
     CDS             complement(19000..19545)
                     /gene="syd"
                     /locus_tag="RH222_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV3797835.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19613..20461
                     /gene="queF"
                     /locus_tag="RH222_002847"
     CDS             19613..20461
                     /gene="queF"
                     /locus_tag="RH222_002847"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV3797836.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20573..21937
                     /gene="ppnN"
                     /locus_tag="RH222_002848"
                     /gene_synonym="ygdH"
     CDS             20573..21937
                     /gene="ppnN"
                     /locus_tag="RH222_002848"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV3797837.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5434 a         5893 c         5240 g         5561 t
ORIGIN      
        1 ctttcgtgtg ttcctttgtt gcagcccaac caactattgt cccacaatta cagcaacaag
       61 ttacggatct tacgagtagc ttaaactcac aagaaaagaa agaactgacg cacaagttag
      121 aatctatttt caataatacc caagtgcaaa ttgctgtatt aattgttccc acaaccaaag
      181 acgagacaat tgaacaatat gctacaagag tttttgacaa ttggcgttta ggagatgcca
      241 aacgtaatga tgggatactg atcattgttg cctggtcaga tcgtactgtc cgcatcaagg
      301 taggttatgg gctggaagaa aaggtaaccg atgctctggc tggagatatc atccgtagca
      361 acatgatacc cgcatttaaa caacaaaaat tagctcaggg attagagcta gctataaacg
      421 ctttgaataa ccaactcact tcacaacacc aatatccgac taatccttca gaaagtgaat
      481 cagcgtcttc cagtgatcat tattactttg ctattttttg ggtatttgca gtgatgttct
      541 tccctttctg gttttttcat caaggcagta atttttgtcg cgcatgtaaa agtggagtct
      601 gtatttcagc aatctatctt ttagatttat tcctgttctc cgataaaatt ttttccattg
      661 ctgtattttc cttctttttt actttcacca tatttatggt ctttacctgt ttatgtgtac
      721 ttcagaaaag agcatctggt agaagttatc attcagacaa cagcggttcc gctggaggct
      781 cagatagcgg cggatttagc ggcgggggcg gttcttctgg aggtggcggc gcatccggcc
      841 gctggtaact caaccataaa aaatgccagc ccggaggctg gcatttttaa atcagataaa
      901 gtcagtctta tgcctggcct ttgatctctt tacgaccgtt gtacggtgct ttttcgccca
      961 gagcttcttc gatacgaatc agctggttgt atttagcaac acggtcagaa cggctcatag
     1021 aaccagtttt gatctggcct gcagcagtac caacagccag gtcagcgatg gtagcgtctt
     1081 cagtttcgcc agaacggtga gagataactg cagtgtagcc agcatctttc gccatcttga
     1141 ttgcagccag agtttcggtc agagaaccga tctggttgaa tttgatcagg atggagttag
     1201 cgataccttt ttcgatacct tctttcagga tcttggtgtt ggttacgaac aggtcgtcac
     1261 caaccagctg gattttgtcg cccagaactt tggtctggta tgcgaaaccg tcccagtcag
     1321 attcgtccag accgtcttcg atagaaacga tcgggtactg tttggtcagt tcttccagga
     1381 agtgagtgaa ttcttcagag gtgaacgctt tgttgccttc gccagccaga acgtatttac
     1441 catctttgta gaattcagaa gctgcgcagt ccatcgccag agtgatgtct ttgcccagtt
     1501 cataaccagc agctttaaca gcttcagcga taacagccag agcttcagcg ttggaaccca
     1561 ggttcggcgc atagccacct tcgtcaccaa cagcagtgtt catgcctttc gctttcagaa
     1621 cttttgccag gtgatggaaa acttcagaac ccatgcggat ggcttctttc acagttttcg
     1681 cgccaaccgg ctgaatcatg aattcctgga tatcaacgtt gttgtcagcg tgctcaccac
     1741 cgttgatgat gttcatcatc ggaaccggca tagagtattt gcccggagta ccgttcagtt
     1801 cagcgatgtg ctcgtacagc ggcatacctt tagcagctgc agcagctttg gcgttagcca
     1861 gagatacagc caggattgcg ttcgcgccga atttggattt gttttcggtg ccgtccaggt
     1921 cgatcatgat cttgtcaatg ccagcctgat ctttagcatc tttgccaatc agcgcctgag
     1981 cgatcgggcc gtttaccgca gcaacagctt tggttacgcc tttacccagg aaacgggatt
     2041 tgtcgccatc gcgcagttcc agagcttcac gggaaccagt agaagcacct gacggagcag
     2101 ctgccatacc gacgaaacca ccctccagat gtacttcggc ttcaacagtc gggttaccac
     2161 gggagtcgat gatttcacga ccgatgattt ttacgatttt ggacattagg ttttcctcaa
     2221 gtcactagtt aaactgaaac tccagacaaa caacgcgtac ccagggtacg cgttgccgct
     2281 ctaacttttt tacttacttc gcctgacgtt tctggaactc gctggcggct ttcacaaagc
     2341 ctgcaaacag cgggtgacca tcacgtggag tagaagtaaa ctccggatgg aactggcaag
     2401 ccacgaacca cgggtgattc ggaacttcga tgatctcgac caactgatca tccccggaac
     2461 ggcccgcaac gcgcagacct gcatcttcaa tctgtttcaa cagcatgttg ttgacttcgt
     2521 aacggtgacg atgacgctca acaattgtcg gcgcattgta cagctggcga accaggctat
     2581 cgtcaaccaa ctggcactgc tgtgcgccga gacgcatggt accgccgaga tcgctcttct
     2641 cgctacgaac ttcaacgttg ccgttttcat cgcgccactc ggtaatcagc gccacaaccg
     2701 ggtacttaca gtctggcaca aattccgtag agttggcgtt ctccatgttg gcaacatggc
     2761 gagcgtaatc aattaacgcc acctgcatac ccaggcaaat gcccagataa ggaatattgt
     2821 tctcacgcgc aaaacgcgcg gtcgtaatca tgccttctac gccacgatag ccgaaaccgc
     2881 caggtacgag gattgcgtcc agacctttaa ggatttcaac gccgcgcgtt tcaacatctt
     2941 gtgaatcgat cagtttgatg ttgacgctga cacgattctt cagcccaccg tgtttcagtg
     3001 cttcgatcac tgatttataa gcatccggca gttcaatgta cttgccgacc ataccgatgg
     3061 tgacttcact taccgggttc gcttcttcga agataacctg ttcccattcg gacagattcg
     3121 cttccgggca gtttaagctg aatcgtttac aaatataatc gtccagcccc tgagatttca
     3181 acaggcccgg aattttatag atggaatcga cgtctttcag agaaataacc gctttttccg
     3241 gaacattaca gaacaatgca atcttcgcac gttcgttcgc cggaacagcg cgatctgaac
     3301 gacaaatcag gatgtcaggc tggataccga tggagagcag ctcttttaca gagtgctgag
     3361 tcggtttggt tttgacttca ccagacgctg ccatgtacgg caccagcgtc aggtgcataa
     3421 acagagtgtg ctcacggcca atttcaacag ccatctggcg aatcgcttcg aggaacggca
     3481 aggattcgat atcacctact gtaccgccga tttctaccag tactacgtca tgaccttcgc
     3541 caccttccag cacgcgctct ttgattgcgt tagtgatgtg cggaataacc tgcacggttg
     3601 cgccgaggta gtcaccgcgg cgttctttac gcagaacgtc agagtagata cgacccgtgg
     3661 tgaagttgtt gcggcggctc attttggtac gaatgaaacg ctcgtagtgc cccaggtcca
     3721 ggtcggtttc agcgccgtct tcagtaacga acacttcccc gtgttggatt gggctcatag
     3781 tacctggatc gacgttgatg taaggatcca gtttcatgat ggtcacattg aggccacggg
     3841 cttcaagaat ggctgcgagg gaggctgcgg caatgccttt acccagagag gatacgaccc
     3901 cgccggtcac aaaaatatag ttcgttgtca tgctgaacct gagaagttag gttgaaaaga
     3961 cgatggaata accaggacgg gaaagcagta tacccgaaca tgacctgtgc cacaaacttt
     4021 cattatccct cctcttcgcc agcgcactat tgaaatcagg agtgagaaaa tagccccttt
     4081 ggggtaaatg tttttgacgc aaatcaagcg cttgtcattt aaaaaatgac acaaatggcg
     4141 cttgaccgcg taattccctt agagatcaat ttcctgccgt tttacctgtt gccagacttc
     4201 ttccattgtt tcgaggtcaa cacctgtcat ttccagtcca cgcgcggcaa caatacgctc
     4261 cacttcgcga aaacgacgct caaatttttc gttcgctttt tgcaatgcga tttccgcttt
     4321 tgtccctaaa tgacgagcca gattgaccgt ggcaaacagc aggtccccca tttcctcctc
     4381 cagtttagcc tggtcgacaa cagcctgccg cgcttcgtac atcacctcgt cgatctcttc
     4441 gtagacttta tcgactaccg gaccaagcgt cgtccaatcg aagccaacgt tggcgcaacg
     4501 tttctggatt ttttgcgcac gcattaaagc cggtaaacta cgaggaatat cgtccagcgc
     4561 cgaatgctgc gctttctgcg cgcgctcttc ggttttgatt tgctcccaac gggcaagcac
     4621 ttcactactg ttttcggcag aactatcagc aaaaacatgc ggatggcgac gctctaattt
     4681 atcgctaata gcagcgcaaa tatcattaaa gtcaaagcgc ccttcttcct gagccatttg
     4741 cgcgtaaaac accacctgga atagcagatc gcccagttca ccgcgaagat cgtcaaaatc
     4801 ttcacgggcg atggcgtcca gcacttcgta ggtttcttca agggtgtaag gcgcaatggt
     4861 ggcaaatgtc tgctctttat cccacgggca gccgttttcc ggatcgcgca ggcgctgcat
     4921 aatagtgagc aaacggtcga tttgattcat tgaattgtcc tgaaaattgc gggtctgtca
     4981 ggtggaaacc tgtgaccaga atagaagtga gttagtaaca ctacccaatc agtacgttaa
     5041 ttttggcttt aatgagttgt aattcctctg gggcaactgt tcctttcttc gttgctcctc
     5101 ttgcccgcca ggcgatactt tttacctgat cagctaacgc tacgccatca cgttcctgac
     5161 cggataaaac aacttcgaac ggatatcctt ttgattgcgt tgtacaagga acacacagac
     5221 acatacctgt tttgttgttg tacatgaaag gactcaggac aacagctgga cgatgtccag
     5281 cttgctcgct accttttgtc gggtcaaaat caacccaaat cagatcgccc atatcgggta
     5341 cgtatcggct taccattacc agacttcctt atctttcggc tctccccagt cgatattctc
     5401 gtggaggttt tccggcgtga tgtcgttgac cagttcagca agcgtaaata cgggctcttt
     5461 acgcactggc tcaataatta atttgccatc caccaggtca atcttcactt catcatcaat
     5521 attcagattg agcgcctgca ttaacgtagc cgggatccgc accgccggtg aatttcccca
     5581 acgctttacg ctactgtgga tcataaccct ttcctcaaac cgctatcata tgtagataca
     5641 gtatatatca atctacattg tagatacgag caaatttctg cctaactccc gtgcaaccga
     5701 cgcgcgtcga taacatccgg cacctggttg agtttaccca gcacgcggcc cagcacttgc
     5761 aggttgtaaa tctcaatggt catgtcgatg gtcgccagtt gctgtttggt gtcgctacgg
     5821 ctggcaacgc caagcacgtt caccttctcg ttggcgagaa tggtcgtgat atcacgtaac
     5881 aacccactac gatcattagc caccacgcgg accaccagcg aatatccggc ggagtagctc
     5941 tcaccccata ccgcgtcaac aatgcgttct ggcgcatggg agcgcagttc cgccagttgt
     6001 tcgcaatcgg cgcggtgtac tgaaataccg cgtccctggg taatgaagcc gacaatctca
     6061 tctccaggaa tcggctggca gcagcgcgcg atgtggtgca tcaggttacc aacaccttca
     6121 actaccacac gaccgttatc tttactgcgg ttttgcggtg tgtagctttt ttgctgaagc
     6181 tgtttcagcg cggcggcgtc ctgctcttcg gcactcggct tattaaattg cgattgcagg
     6241 aagttcacca tctgattgag acggatatcc ccgccaccaa tcgccgccag caactcgtcg
     6301 acatcattga agttgtaacg cggcaacaga tgtttttctg cttctttcag gctgatcccc
     6361 agatgttcca gctcgtcgtc aaggatttgc cgcccagcca gaatgttttt gtcacggtcc
     6421 tgtttacgga accaggcgtg aattttcgaa cgcccacggc tggttgtgac gtaaccgagg
     6481 tttgggttta accagtcacg gctggggttc ggctgtttct gggtgataat ttcaatctgg
     6541 tcgcccatct gcagctggta ggtgaacggc acaatgcgcc cgccaatttt tgccccgatg
     6601 cagcggtgtc cgacatcact gtggatgtgg taagcgaagt ccagcggcgt tgatcccgca
     6661 ggcaaatcaa cgacatcacc tttcggcgta aagacgtaca cccggtcgtc aaagacctga
     6721 ctacgtactt cgtcgagcat ttcgccggaa tcagccatct cttcctgcca cgcaatcagt
     6781 ttacgcagcc aggcaatccg gtcttcatgt cccgaacgtg cgccgccagc agccgcgccc
     6841 tctttatatt tccagtgcgc agcaacaccc aactctgcat cttcatgcat ctgtttggtg
     6901 cggatttgga tctcaacggt ttttccaccc ggccccagaa ccacggtatg aatagactga
     6961 taaccgtttg gtttcgggtt agcgacgtaa tcgtcaaact catccggcag gtggcgatag
     7021 tgagtgtgca ctatccccag tgcggcataa caatcctgta aacgctcggc gacaatacgt
     7081 accgcacgca catcaaacag ctcatcgaag gcgaggttct ttttctgcat tttacgccag
     7141 atgctgtaga tgtgtttcgg acgaccatac acttcggctt taacgccttc agctttcatc
     7201 tcagcgcgca gatgaccgac aaactcttcg atatagtgtt cgcggtcgag acggcgttca
     7261 tgcaacagtt ttgcgatgcg tttgtattcg gtcgggtgca gataacggaa gcagtaatct
     7321 tccagctccc atttcagttg cccaatccca agacggtttg ccaacggcgc gtagatattg
     7381 gtgcactctt ttgccgccag tacgcgttca tcttccggcg catcttttac ttcccgcaga
     7441 tgagcaatac gctccgccag tttgatgacc acgcagcgaa aatcatcgac catcgccaat
     7501 aacatccggc gaacgttatc gacctgttcg gaggaaacag aatcagtgtg cgtcgctttc
     7561 agctggcgga tcgccgccat atcacgcacg ccgtgaataa ggttaacgac cgacttaccg
     7621 acgctctcac gcagcacatc ttcgctgact acgttggcat ccgccagagg gaaaagcagc
     7681 gccgcccgca gcgtgtcaat gtccatactt aatgtcgaga ggatctccac catctcaaca
     7741 ccacgccaca ataacagact ggcatccgga tgcccctgcg tctgttgcag acaatacgcc
     7801 caggtttcgg ctaagcactc acacgacttc tggctggtaa tacccagact tgcgatccat
     7861 ttttccggat caaattcacc agccttattg atatgtgcac ttcttaccgc aaccatcgtc
     7921 ctctccttta gggaccagac ctgccgaaat cggcaaatcg caactattta acgcgcgaga
     7981 aaagtaccat cgattccaga tgtcccgtgt gtgggaacat atccagcatc gccagtcgcg
     8041 caatggtata tcctgctttt aataacgctt cgctatcccg agccagcgtt gcagggttac
     8101 aggatacata aactatacga ataggttcca gttttataat ttgctgcata acacctgcgg
     8161 cacctgctcg cgccgggtcc agcaacactt tatcgaagcc gtttttcgcc cacggctgct
     8221 ttgtgacatc ttcttcaaga ttttcgtgat aaaacgtcac attctgtaag ccattaagac
     8281 gcgcattctg ctggcctttt tccaccagcg ccggaacacc ttctacaccg accacactgg
     8341 cagcttgtgt cgccaatggc agtgtaaagt tgcccatacc gcagaacaga tccagtacgc
     8401 gatcttcagg ttgcacatcc agccattcca acgcacgcgc taccattttt tggttcacac
     8461 ccgcattgac ctgaataaaa tcgcgcgggc taaaagttaa gcgcaacccg tttgagtcat
     8521 accagggcat ctcaccagag acggtttcga gtatctcact atcgggggcg agatacagat
     8581 ccaggccttc agaatgcgaa aagcgttcca gtttttcgcg atctgccgaa cttagcggtg
     8641 cggtatggcg caaaatcatc agcgtgccgc tggttgcctg taccagttca acatgaccaa
     8701 gatggcgcat agcttgtaag ctgcccagac atgccctgac tttgggcagc aatgcttcaa
     8761 gttggggcgc taaaatgggg cattgtttga cgtcgacaat gtcactggag cccgctttgc
     8821 gaaaccccat ctgaagttgc tgtgttttcg gtaagtagtt cagacttaaa cgcgcgcgac
     8881 ggcgatagcc ccagggaaca tcggcgatca cttcagagac atcgtgtttc attaatcggg
     8941 cgagtgccgc acttttgctt cgctgctgta aatccacgct ggcgtgttgt tgctgacagc
     9001 caccgcatac gccaaaatga ggacagcgtg gcgtttcgcg ttccgggcta tcgcttaacc
     9061 ggcgtacgac tttagcgcgg gcatactgtt ttttatcttc agtaacagta acttccgcgt
     9121 tttcctgcgg caataatccg gggataaata gcgttttgcc gttatgtcgc gccacgccct
     9181 gaccaaaaga gtcgaggtcg ttgactgaaa cggttatgat ctgacgcgtc gtcgtgcgtc
     9241 gttttgcaga gtagaattgc gccattggcg agactttctc aatttaacag tgtgacctta
     9301 attgtcccat aacggaactc catgaccaac tacagcctgc gcgcacgcat gatgattctg
     9361 atcctggcac cgaccgtcct tattggttta ttgctgagta tctttttcgt cgtgcatcgc
     9421 tataacgact tgcagcgtca actggaagat gccggtgcca gcattattga gccgcttgca
     9481 gtttctactg aatatggcat gagcctgcaa aatcgcgaat ctatcggtca gttaataagc
     9541 gtactgcatc gtcgccattc cgatattgtt cgcgcgattt cggtttatga tgaaaataac
     9601 cgactctttg tcacctccaa ttttcatctt gatccctcat caatgcagct cggcagcaac
     9661 gtgccgtttc ctcgccagct cactgtcact cgtgacggcg atattatgat cctccgcacg
     9721 ccgattattt ctgagagtta ctcccccgac gaatcgccca gtagcgatgc caaaaatagt
     9781 caaaatatgt tgggatatat tgcgctggag ctggatctta aatcggttcg cttgcagcaa
     9841 tataaagaga tctttatttc cagcgtgatg atgctgtttt gtatcggtat tgcgcttatt
     9901 tttggctggc gcttaatgcg cgatgtaacc ggtccgattc gcaacatggt gaataccgtc
     9961 gaccgcatcc gtcgcgggca actcgacagc cgggtggaag gatttatgct cggcgagctg
    10021 gatatgctga aaaacggtat caactcgatg gcaatgtcgc tggctgctta tcacgaagag
    10081 atgcagcaca atatcgacca ggcgacgtcc gatctgcgtg aaacgctgga gcagatggaa
    10141 attcagaacg ttgagttgga tctggcgaaa aagcgcgccc aggaagcggc gcgtattaaa
    10201 tccgagtttc tggcaaatat gtcacacgag ctgcgtacac cactgaatgg tgttattggc
    10261 tttacccgcc tgacgctgaa aacagaatta acaccaacgc agcgcgatca cctgaatacg
    10321 attgaacgtt cggcaaataa tttgctggca attattaatg atgttctcga cttctcgaaa
    10381 ctggaagcag gtaagctgat tctggaaagt attccattcc cactacgcag cacgctggat
    10441 gaagtcgtta ctctgctggc acattcttct cacgataaag ggttggaact gacgctcaat
    10501 attaaaagcg acgtgcctga taacgtgatc ggcgacccgc tgcgattaca gcaaatcatc
    10561 actaacctgg tggggaatgc aattaaattc accgagaatg gcaacattga tattctggta
    10621 gaaaaacgtg cgctgagtaa taccaaagtg cagattgaag tgcagattcg ggataccggc
    10681 attggtattc ctgaacgcga tcaatcgcgc ttattccagg ccttccgaca ggctgatgcc
    10741 agtatttccc gccgtcatgg tggcaccggg ctggggctgg tgattacaca aaaactggtt
    10801 aatgaaatgg gcggcgatat ttcgttccat agccagccga atcgcggttc aactttctgg
    10861 ttccacatta atctcgatct gaacccgaac attattatcg aagggccatc cacccagtgc
    10921 ctcgcaggta agcgcctggc ctatgtcgaa ccaaactccg cagcagcgca atgcacgctg
    10981 gatattttaa gtgaaacgcc gctggaagtg gtttatagcc caacgttctc cgcgctgcct
    11041 cccgcgcatt acgacatgat gttgttaggc atcgcggtga ccttccgcga gccgctaaca
    11101 atgcaacatg agcgattagc gaaagcggta tcgatgaccg atttcctgat gctggcactt
    11161 ccttgccatg cacaagtcaa tgctgaaaaa ctcaagcaag atggtatcgg cgcgtgtctg
    11221 ctgaaaccat taacacctac acgcctgttg cctgccctga cggaattttg tcatcacaaa
    11281 caaaacacgc ttttgcctgt aaccgatgaa agtaagctgg caatgacagt catggcggtt
    11341 gatgacaacc ccgctaacct gaaacttatc ggcgcattgc tggaagatat ggtgcaacat
    11401 gtggaacttt gcgatagcgg gcatcaggcg gttgaacggg cgaaacagat gccgttcgat
    11461 ttgatcttaa tggatattca aatgccggac atggatggca ttcgggcctg cgaactcatc
    11521 caccaactcc cgcatcagca acaaacgccg gttatcgcgg taacggcgca tgcaatggcc
    11581 gggcaaaaag agaagctgct tggcgcaggg atgagcgatt atctggcgaa accgattgaa
    11641 gaagagcgat tgcataattt gttgttgcgc tacaagcctg gcagcggtat ttcctctcgc
    11701 gtcgtgacgc ccgaagtcaa cgaaattgtg gtgaacccga atgcgaccct cgactggcaa
    11761 ctggcactac gccaggcagc aggaaaaacc gatttagcgc gcgatatgct gcaaatgtta
    11821 ctcgatttcc tgcctgaagt tcgcaacaaa gttgaggaac agctggttgg agaaaacccg
    11881 gaaggcctgg ttgatttgat tcataaactg catggcagtt gcggctatag cggtgtgcca
    11941 cgtatgaaga atctctgcca actgatcgaa caacagctac gtagcggtac taaagaagaa
    12001 gatttggaac cggagctgct ggaactgttg gacgagatgg ataatgtcgc gcgcgaagcc
    12061 agcaaaattc tcgggtaatg gatgtcttgt cgagtagcaa tcggcttttt atgacgctgg
    12121 tttcagattg gtgacaaagt gcctgtgttt atgccggatg cggcgttaac gccttatccg
    12181 gcctacaaaa ccaattaaat tcaatgaatt aaaaaatgat gtagacctga tacatagcac
    12241 aacaggctat tttgcgttta gcatcagtct caaaccggct ccagatagag ccggttttgg
    12301 ttttctgtct taacgcacca tgcacgggcg cttgttatcg aacgtccagc caggaatcag
    12361 atactgcatt cccatcgcat cgtcacgcgc gccaagcccg tgtttctgat acagctcatg
    12421 ggctttcatc acttgatcca tatcgatttc tacacccagc cccggttttt ctggcacctg
    12481 taccagcccg cctttgatct caaacggttc tttggtcagg cgctgattgc cttcctgcca
    12541 aatccagtgc gtatcaatag cagtaatttt acccggtgca gcggcggcaa catgggtaaa
    12601 catcgccagg gaaatatcga agtggttgtt agagtgtgaa ccccaggtca ggccaaattc
    12661 atggcacatt tgcgccacac gtaccgaacc ttgcattgtc cagaaatgcg gatccgccag
    12721 cgggatatca acggattgta acgagagcgt atggcccatc tgccgccagt cggtggcgat
    12781 catattggtt gcggttggca ggccagttgc gcgacggaac tctgccatca cttcacgccc
    12841 ggagaaacct tgctccgcac cacacggatc ttctgcataa gccagtgacc ctttcaggta
    12901 tttaccaatt ttaatcgctt cgttcagcga ccaggcaccg ttaggatcga gcgtaatacg
    12961 cgcctgcggg aaacgtttcg ccagtgccac aatagactcg gcttcttctt ccccggccag
    13021 tacaccgccc ttcagtttga aatcgttgaa gccatatttt tcatatgccg cttccgccag
    13081 gcgcaccacc gcatccggcg tcatcgcttc ttcatgacgc aggcgatacc agtcgcatga
    13141 gtcatccggc tggctttgat acggcagcgg tgtggctttg cgattaccga cgaagaacag
    13201 ataaccgagc atttcgactt cgctacgctg ttgaccatcg cccagcagcg atgccacgtt
    13261 tacccccaga tgctgcccca gcagatccag cattgccgct tctatcccgg taactacatg
    13321 aatagtggta cgcaaatcaa atgtctgcaa accgcgccca ccagcatcac gatcggcaaa
    13381 agtattacgc accagcgtca gaacgttttt gtattcaccc agcgttttac ctaccaccag
    13441 cggaatcgca tcttccagcg ttttacggat tttctcgccg ccgggaattt cccctacgcc
    13501 agtgtgacca gaattatctt tgataatcac aatattacgc gtaaagaacg gtgcgtgtgc
    13561 accactcaga ttcatcagca tactgtcatg acccgccacc gggatgacct gcatttcagt
    13621 aacaacaggc gtcgtaaatt gagaactcat atttatgtcc tttttcagaa tcaatgacgg
    13681 ccgaaaacgg gacgtttacg gtcaaaggtc cagccgggga tcaggtactg catcggacct
    13741 gcgtcgttac gcgcaccgcc aggcagacgt ttataggcct catgtgcctt ttgtacctgt
    13801 tcccagtcca gttccacgcc cagaccgggc gcatcaggaa cggcaatttt tccgttttta
    13861 atctccagcg gatttttggt caggcgacaa tcgccctcct gccaaatcca gtgggtatcg
    13921 atagcggtag gattacccgg tgccgccgcg cccacatggg taaacatcgc cagagagata
    13981 tcgaaatggt tattagaatg gcagccccag gtcagccccc agtcgtcgca aagctgcgcc
    14041 acacggactg caccggaaag cgtccagaag tgcggatcgg caagtggaat atctaccgca
    14101 ttgagcatca ccgcatgacc catttcgcgc cagttggtgg cgatcatgtt agtcgcgacg
    14161 ggcaagccgg tcgcccgccg aaactccgcc atcacttcac gtccggagaa gccctgttct
    14221 gcgccgcatg gatcttcggc ataggtaaga acatcattca gccctttgca caaagaaatg
    14281 gcttcatcaa gcagccaggc accgttggga tcaacggtaa tccgcgcatc tgggaagctt
    14341 ttcttcaatg cacgaacagt gtcgatttct tgctcgccag gtaacacccc gcccttaagt
    14401 ttgaaatctt taaagccgta gcgatcctgt gaggcttccg ccagacgcac aacggcttcg
    14461 ctgttcatcg ctttctgatg gcgcaactga taccactcat ggttgcccgg cgtattttcc
    14521 agataaggaa gatcggtttt ggtccgatca ccgatataaa acagataacc gaggacggta
    14581 atagtctcgc gttgcttgcc tggccccaac agttcgcaga ccggaacatt cagcgcctta
    14641 cctagcaggt caagcaaggc ggcttccagc gccgccacgg cattaacgcg caattcaaaa
    14701 gtccaggcac ctttgccgaa ggtatcaaaa tcggctgcct gattaccttt atgcacctgc
    14761 tgaaccactt tattcaggcg cgcaacttcc tggcccagaa ccatcggaat agcatcgacc
    14821 agtgtttgat aaatcacctc tccgcccggc gcttcaccaa tgccggtatg cccggcgtta
    14881 tcggtgagta ccacaatatt gcgggtgaaa tatgcgttat gtgccccacc aatattaagc
    14941 aacatgctat cgtgcccggc gaccggaatg actttcatat cagtaataac aggactggat
    15001 tgtgtcgtca tcttattgcc ccgcgacagg tttcagctcg atacgcttga tatcgcccac
    15061 cagcaccagg tagctcagta ccgcgattaa ggcatgaaca ccaacataaa tcagcgcccc
    15121 attaaaggag ccagtcgtgc caacgatata accaattgcg attggcgtga cgatgccaga
    15181 aatgttaccg aacatgttga acaggccacc ggaaagaccg ctgatctctt ttggcgcggt
    15241 atctgccatt actgcccaac ccagcgcccc gatgccctta ccgaagaagg ccagcgccat
    15301 aaagccgatg atcatccact caacgttgac gtagttgcag aacaccatca ccatcgacag
    15361 caacatgccc attacgatcg gtgttttacg cgcaatgttc agcgatcccg tgcggcgcat
    15421 cagccagtcg gaaataatcc cacccagcac accgccgata aaaccgcaaa ccgccggaac
    15481 ggaagccaca aagcccgctt tcagaatcga catcccgcgt gcctgcacca gataaaccgg
    15541 gaaccaggta ataaagaagt aagtcagggc gttgatacag tactgaccga tataaacgcc
    15601 gatcatcatc cgtgacccga gcagctgttt gatctgcccc cacttcacgc tgaacggcac
    15661 tttaacttta gtgttttgct gatccatatt gatcagcgcg ccacccgcgg cgatatactc
    15721 cagctctttc tgatttacgc ccggatgttg atttggctcg tggatgactt tcaaccagat
    15781 aaagctgatg acaatgccca gaccgcccat aaagaagaag acgtgtgacc agcccacttc
    15841 atgcgtcagc cagcccataa tcggcgcgaa gataaccgtt gcgaagtatt gggcggagtt
    15901 aaaaatcgac accgccgttc ccctttcctg cgccggaaac caggccgcaa caatgcgact
    15961 gttgccgggg aaagatggcg cttcagcaag cccgaccagg aagcgcagcg taaacagggc
    16021 aacgataatg ccgaatccac taaagatatc gacgaagcct tgcagcaagg taaacatcga
    16081 ccagataaag atcgaccaga agtagacgcg ttttgaacca aaacggtcca gcaaccagcc
    16141 accagggatc tgcccgataa cataagccca tgagaaggca gagaacacat agcccattcc
    16201 cacgggatca aggccgatat ctttggccat ttccgaaccg gcaatagaaa gcgtagcgcg
    16261 gtcgccgtag ttgaaggatg tgacgataaa caacatcacc actatccagt aacgagcatt
    16321 cgtgcgtttt tccacactgc tcgcagcctg acttaaagaa ctcattgttg cactcctgaa
    16381 aattcgcgtt agccacgctc actctggact gcgacatagc caggaaatca gaggtgacgt
    16441 agggtgtttt ttgccgtttt tataggtcgt tcgccgaata cggcgcgtgt ttatatctgg
    16501 caatagcagt ataaaaagcg cgccatagcg gctcaccgtg caacaacaca acattaatgc
    16561 gttcaatgag gcccgatttt ggcattagcc ctggacggtg gaatccactt cacggaaatg
    16621 aaaacaagaa caagaaagga agggttaaaa cgaagaaata aagaagagta tgaaatggat
    16681 cgcttgactc caggcaaacg ccagtaaaaa tccgcgctat gaagcagttt ttactggcat
    16741 ttgcctgaaa agattcgatt cagcaccgct aaaacgacat ttaccgctcg ctgaacatat
    16801 caggacaaca gcgtgcccca ctgttcgacc cacggatttg attccgtttc cggttccggg
    16861 ttctcactgg cgtcaatcaa cagtatttcg ccaacccgct gagcgctctg ttcctgcaac
    16921 aaggcatcga actgtttgcc gccattgcag aaatttacat aactgctgtc gccgagcgca
    16981 atcacgccat aacgcagatt cggctggaag cccagactat ctttgattcc ctgaaagagc
    17041 ggcacaatgc tatcaggaag gtcgccctgc ccggtcgtgg acgtaaccac cagaacatac
    17101 ttatcctgat aaggcagcca gtcgcttaat tcaggatctt caaataccgt tgctttgtgg
    17161 ccctgtgcgg tcagaattgc ttctgcttct tccgccacta acagcgaatt tccgtacatg
    17221 gtgccgacaa aaataccaat ttccgccata ccgttttctc cctggattag gaacttatct
    17281 ctccatcctg acccgatggc gcactgaact caaccctttc attttcagga agcagaccgc
    17341 gccagccaaa ttgtgataac gcctgcatcc aggtgtcgtc caggcccgcg tgaatagtca
    17401 gcggctcgcc agtaaaagga tgcgtcaatg acaactgact ggcatgtagc attaaccgct
    17461 ggaggccaaa atgctcagca ccgctgcgat tctggcgtaa atcgccatgt ttgctatcgc
    17521 caataatcgg atgacgcaaa tgggcaagat gtcggcgaag ctgatgtttg cgtccggttt
    17581 tcggctccag ttccaccagg ccgtagcgcg tggtcgggta acgtccggtc gctaccggca
    17641 tttctacggt cgccagaccg cgataatgcg tcactgctgg ctgcgagcct ttatcttcgc
    17701 gggcaaattt atcagcgatt ttgtccagtt cttccaccag tggataatcc agcaccgctt
    17761 cttccatcaa ccagccgcgc acaatcgcat ggtaacgttt ctggatttgg tgctgttcaa
    17821 actgttgtgc cagcagccgt ccggcctcgc tggataatcc catcaacaac acaccagaag
    17881 tgggtcggtc cagacgatga gcagtaaaaa catgctgtcc tatctggtca cgcacggttt
    17941 gcatgaccac tactttctcg tcgcgatcca gccagctacg gtgaaccagc cagccggagg
    18001 gtttatttac cgcaaccagc cattcatcct gatagagtat ttccagcatt agctcgcatc
    18061 atccgcaaaa agagcatcca gtttttccaa ctcagccaga ataagcgcgc gttgcggatg
    18121 gtccgtcgcc agcgccattt cataataggg tgcaacagca aaagcgcccg gtaacggctg
    18181 gttgttattt aacaaatcgt gcattcgcgg gatcagcacc cactgcaacc actccagtgg
    18241 ttccatggtg tccataaaga acggttgggt actattaaat tgatgcggct ggggttcatc
    18301 gtttcgccag tgctgatgtt cacgcagtaa cgcttcaagc gcctgcaact ggagacgaac
    18361 gcggtcatga gtggtcataa agcaacctca ataagaaaaa ctgccgcgaa gaatagcagg
    18421 aataaaaaaa gggagcactg tattcacagc gctcccggtt cgtttcgcag cattccagct
    18481 acttttgttg ctccctgctc atccttgaca acttttcctc tggccttgcg gccaatcgtt
    18541 catcctgaac tattgcttcc tgctcacacc accccgatgt gatacttcat cctgaagtgt
    18601 ccctggcctt cctgacccac cgaatcatcc tgaccggttc tcattctcca tcctggaggt
    18661 gtcctttaac gcgtcctgcg tcatcctctt cgcttcatcc agaaaccttt ccctgaaaca
    18721 ccatcctggt gtgtcctgca gaagtgtcat catcctgatg ttcacttcgt tgtctgactc
    18781 cctgtcgacg aagatagaat cgtctttttc gcgaagtctt acaaggcgct tacagacaat
    18841 gccttaagga aaatttcata tgaaagttaa gaattaacaa tcaacatttt gattttaaat
    18901 gaaaataacg attcttatct ttttaagtat tcgcttattt cctggcgatc tcctacagag
    18961 tctgtaagag atctctcaca accagatggg gtatttggat tacagaagag gcttaagttg
    19021 attaaggaat tccgcaagat ttgaggctaa atgcgtccgt ttacgtgttc ccagcgtctc
    19081 tttgcacact tcaccggaga gattacacac cgagatgacc tctaactctt cttccagcgt
    19141 cgcgataaat agcgtgggcg gcagttttag ccttttctga gtcaccagat gaccaatcag
    19201 attttcctgc acccgacgga agtcgtcttc actccaggtt tgcagcaatg tcagtttgat
    19261 atcgccaaac tgcgcgtgca tatccccggc aaactgagtc gtataaaagg tatgaattgt
    19321 gggttgtatc acaatatcaa aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa
    19381 cggctggggt tgccagtata cggcatcttc ggtagttgaa ataatgcacg gtgaaggaac
    19441 accgtacagt tcctcactta acggccagct tttatgctct tcatgccacg catcgcagta
    19501 gcgtgcagta aaatctttca gggcttgtgc ggtcaaatcg tccactaatt tctctcttca
    19561 cttaaaccag atacacttgt cgtttagttt atctggttta tgacggtgaa acatgtcttc
    19621 ttatgcaaac catcaggcac ttgcgggcct gactcttgga aaatcaaccg attaccggga
    19681 tacctatgac gccagcctac tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg
    19741 tctgaaagcg gataacctgc cttttcacgg tacggatatc tggacgctgt atgaactttc
    19801 ctggctgaat gcgaaaggtt tgccgcaggt tgccgtcggt cacgttgaac ttgattacac
    19861 cagcgcaaat ctgattgagt cgaagagttt taagctctat ctcaacagtt ttaaccagac
    19921 gcgttttaat aactgggatg aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc
    19981 ccagggtaag gttagcgtgg cgttatatcg tcttgatgaa ctggaaggcc agccgatagg
    20041 tcattttaat ggcacttgca ttgatgacca ggatatcact atcgataact atgaattcac
    20101 tactgactat ctggagaatg ccacctgtgg tgaaaaagta gtggaagaga cgcttgtcag
    20161 ccacctgctg aaatcaaact gcctgatcac ccatcaacca gattggggtt cgatccaaat
    20221 tcagtatcgt ggacgccaaa ttgacagaga aaaactgctg cgttacctgg tctcattccg
    20281 tcatcacaac gagttccacg aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt
    20341 ctgccagcca gaaaaattga gcgtttacgc acgttatacc cgtcgtggcg gtctggacat
    20401 taacccgtgg cgcagtaata gcgattttgt cccatcgacc acaagactgg ttcggcaata
    20461 aattttttct caattttgcg tgctggattc acgcagaagg ttgtgaaagg tcatcaggca
    20521 gggctattgt aatcaaaggg aatgacgata ttcgtcccat aaggagtttt tcttgattac
    20581 acatattagc ccgcttggct ccatggatat gttgtcgcag ctggaagtgg atatgcttaa
    20641 acgcaccgcc agcagcgacc tctatcaact gtttcgcaac tgttcacttg ccgtactgaa
    20701 ctccggtagt ttgaccgata acagcaaaga attgctgtct cgttttgaaa atttcgatat
    20761 taacgtcctg cgccgtgaac gcggcgtaaa gctggaactg attaatcccc cggaagaggc
    20821 ttttgtcgat gggcgaatta ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgaga
    20881 cattctcttc gtttacgggc aaatccataa caccgttcgt tttcccaacc tgaatctcga
    20941 caactccgtc cacatcacta acctggtctt ttccatcttg cgtaacgctc gcgcgctgca
    21001 tgtgggtgaa gcgccaaata tggtggtctg ctggggcggt cactcaatta acgaaaatga
    21061 gtatttgtat gcccgtcgcg tcggaaacca gctgggcctg cgtgagctga atatctgcac
    21121 cggctgtggt ccgggagcga tggaagcgcc gatgaaaggt gctgcggtcg gacacgcgca
    21181 gcagcgttac aaagacagtc gttttattgg tatgacagag ccgtcgatta tcgccgctga
    21241 accgcctaac ccgctggtca acgaattgat catcatgccg gatatcgaaa aacgtctgga
    21301 agcgtttgtc cgtatcgctc acggcatcat tatcttccct ggcggtgtgg gtacggcaga
    21361 agagttgctg tatttgctgg gaattttaat gaacccggcc aacaaagatc aggttttacc
    21421 attgatcctc accggcccga aagagagcgc cgactacttc cgcgtactgg acgagtttgt
    21481 cgtacatacg ctgggcgaaa acgcgcgccg ccattaccgc atcatcattg atgacgccgc
    21541 tgaagtcgct cgtcagatga aaaaatcgat gccgctggtg aaagaaaatc gccgcgacac
    21601 tggtgacgct tacagcttta actggtcaat gcgcattgca ccagatttgc aaatgccgtt
    21661 tgagccgtct cacgagaata tggctaatct gaagctttac ccggatcaac ctgttgaagt
    21721 gctggctgcc gacctgcgcc gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt
    21781 cggtattcgc gccattgaag agtttggtcc ttacaaaatc aacggcgata aagagattat
    21841 gcgtcgtatg gacgacctgc tacagggttt tgttgcccag catcgtatga agttgccagg
    21901 ctcagcctac atcccttgct acgaaatctg cacgtaatct ccgctccccc ggtgacgttt
    21961 gttgccgggt tttctcgttt ttggtcactt actcatcaac tcatttcatt tgttatatga
    22021 atgtttctta ccaccctcat gcgacaaata tcatcacagt taatatgtca tacaatttat
    22081 attgcaacgc aaacgtttcc ctattttcat aaaccgtatt ttatcgct
//
LOCUS       DAPHJE010000073        21977 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPHJE010000073 DAPHJE010000000
VERSION     DAPHJE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.073"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            383..598
                     /gene="essD"
                     /locus_tag="RH222_002849"
     CDS             383..598
                     /gene="essD"
                     /locus_tag="RH222_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV3797838.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            598..1095
                     /gene="rrrD"
                     /locus_tag="RH222_002850"
     CDS             598..1095
                     /gene="rrrD"
                     /locus_tag="RH222_002850"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HDV3797839.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1312..1494
                     /gene="rzoD"
                     /locus_tag="RH222_002851"
     CDS             1312..1494
                     /gene="rzoD"
                     /locus_tag="RH222_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDV3797840.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1585..1878)
                     /gene="iss"
                     /locus_tag="RH222_002852"
     CDS             complement(1585..1878)
                     /gene="iss"
                     /locus_tag="RH222_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDV3797841.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(2241..2435)
                     /locus_tag="RH222_002853"
     CDS             complement(2241..2435)
                     /locus_tag="RH222_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV3797842.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2824..3369
                     /locus_tag="RH222_002854"
     CDS             2824..3369
                     /locus_tag="RH222_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV3797843.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3344..5269
                     /locus_tag="RH222_002855"
     CDS             3344..5269
                     /locus_tag="RH222_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV3797844.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKFARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTEIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5266..5472
                     /locus_tag="RH222_002856"
     CDS             5266..5472
                     /locus_tag="RH222_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HDV3797845.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTVTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5469..7070
                     /locus_tag="RH222_002857"
     CDS             5469..7070
                     /locus_tag="RH222_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV3797846.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            7051..8370
                     /locus_tag="RH222_002858"
     CDS             7051..8370
                     /locus_tag="RH222_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HDV3797847.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8380..8712
                     /locus_tag="RH222_002859"
     CDS             8380..8712
                     /locus_tag="RH222_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HDV3797848.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8768..9793
                     /locus_tag="RH222_002860"
     CDS             8768..9793
                     /locus_tag="RH222_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDV3797849.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9835..10233
                     /locus_tag="RH222_002861"
     CDS             9835..10233
                     /locus_tag="RH222_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HDV3797850.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            10245..10598
                     /locus_tag="RH222_002862"
     CDS             10245..10598
                     /locus_tag="RH222_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HDV3797851.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10610..11188
                     /locus_tag="RH222_002863"
     CDS             10610..11188
                     /locus_tag="RH222_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3797852.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVSRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11185..11580
                     /locus_tag="RH222_002864"
     CDS             11185..11580
                     /locus_tag="RH222_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3797853.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVSLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11588..12328
                     /locus_tag="RH222_002865"
     CDS             11588..12328
                     /locus_tag="RH222_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3797854.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEINVTAS"
     gene            12344..12766
                     /locus_tag="RH222_002866"
     CDS             12344..12766
                     /locus_tag="RH222_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV3797855.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQTEDAGPAEPVSAGKCSMVS"
     gene            12748..13182
                     /locus_tag="RH222_002867"
     CDS             12748..13182
                     /locus_tag="RH222_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV3797856.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDVNEVIPVSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13175..15736
                     /locus_tag="RH222_002868"
     CDS             13175..15736
                     /locus_tag="RH222_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV3797857.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGALTLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFDQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLMLQTELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIEAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GKFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            15733..16062
                     /locus_tag="RH222_002869"
     CDS             15733..16062
                     /locus_tag="RH222_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3797858.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            16062..16760
                     /locus_tag="RH222_002870"
     CDS             16062..16760
                     /locus_tag="RH222_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV3797859.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16766..17509
                     /locus_tag="RH222_002871"
     CDS             16766..17509
                     /locus_tag="RH222_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3797860.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            17407..18054
                     /locus_tag="RH222_002872"
     CDS             17407..18054
                     /locus_tag="RH222_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV3797861.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            18115..21612
                     /locus_tag="RH222_002873"
     CDS             18115..21612
                     /locus_tag="RH222_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV3797862.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDSQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVLFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKQEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPDGTVTVTISDDHNFDRQIIIPPIIFNGIAYSDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGRGSVTLEFKVFHKGNQWAGN
                     ITDCTVIVTKKAASGISIR"
     gene            21682..>21977
                     /locus_tag="RH222_002874"
     CDS             21682..>21977
                     /locus_tag="RH222_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV3797863.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSV"
BASE COUNT         5115 a         5450 c         6717 g         4695 t
ORIGIN      
        1 ttaacggctg ttatttataa gtgctctata atttgaaggt tcaatttaaa tcggctaaaa
       61 ataacactgg aaattatttg ttggttattt gttgaggttt tcttatgtat ttgtggtggt
      121 gttttgaaca ctcggtagca ttctcataaa tatcattcag tggtttacgt acgtaaaaaa
      181 ttggttatgc tgttaagagt ggttacttcg tcacacagct taaacccgcc gtcgagctgg
      241 tttttccatt ttttgagtct cgatattagc tgataactca atacctgagt tattcactga
      301 ctccgagtct gttacgtttc tgcttttttg cgatacgttg tattccctca atttacaccc
      361 gctttgtctg cgaggtgggg ttatgaaatc catggataag ttaacaacgg gtgtcgccta
      421 tggcacctca gcaggtagtg ccggttactg gtttttacag ctgctcgata aagtcacgcc
      481 ctcacagtgg gcagcaatag gtgtgctggg tagcctggta tttggcctgc tgacgtacct
      541 gacaaacctt tatttcaaga ttaaagaaga taagcgcaag gctgcgagag gtgaataatg
      601 cctccatcat tacgaaaagc cgttgctgct gctattggtg gcggagcaat tgctatagca
      661 tcagtgttaa ttactggccc aagtggtaac gatggtctgg aaggtgtcag ctacatacca
      721 tacaaagata ttgttggtgt atggactgta tgtcacgggc atacaggaaa agacatcatg
      781 ctcggtaaaa cgtataccaa agcagaatgc aaagccctcc tgaataaaga ccttgccacg
      841 gtcgccagac aaattaaccc gtacatcaaa gtcgatatac cggaaacaat gcgcggcgct
      901 ctttactcat tcgtttacaa cgtgggtgct ggcaatttca gaacatcgac gcttcttcgc
      961 aaaataaacc agggcgatat caaaggcgca tgtgatcagc tacgtcgctg gacatatgct
     1021 ggcggtaagc aatggaaagg tctcatgact cgtcgtgaga ttgagcgtga aatctgtttg
     1081 tggggtcagc aatgaacaga gtaaccgcga ttatctccgc tctggttatc tgcatcatcg
     1141 tcggcctgtc atgggctgtt aatcattacc gtgataacgc cattacctac aaagcccagc
     1201 gcgacaaaaa tgccagagaa ctgaagctgg cgaacgcggc aattactgac atgcagatgc
     1261 gtcagcgtga tgttgctgcg ctcgatgcaa aatacacgaa ggagttagct gatgcgaaag
     1321 ctgaaaatga tgctctgcgt gatgatgttg ccgctggtcg tcgtcggttg cacatcaaag
     1381 cagtctgtca gtcagtgcgt gaagccacca ccgcctccgg cgtggataat gcagcctccc
     1441 cccgactggc agacaccgct gaacgggatt atttcaccct cagagagagg ctgatcacta
     1501 tgcaaaaaca actggaagga acccagaagt atattaatga gcagtgcaga tagagctgcc
     1561 catatcgatg ggcaactcat gcaattattg tgagcaatac acacgcgctt ccagcggagt
     1621 ataaatgcct aaagtaataa aaccgagcaa tccatttacg aatgtttgct gggtttctgt
     1681 tttaacaaca ttttctgcgc cgccacaaat tttggctgca tcaacagttt tctcctgtcc
     1741 aattcccgaa acgaagaagt gatgggtgat ggtttccttt ggtgttactg ctgtcggttt
     1801 gtttccaaca gtaaacgtct gttgagcaca tcctgtaata agcattgcca gagcggcaga
     1861 aaacaacatt tttttcatct tattatcctg cattgttaaa aacggcagaa tcctatgtga
     1921 caacaattaa acgatagtta aatggattga tgaaaattaa aactatatag gtgtacgctc
     1981 agactattgg aggaagttgg ggacactcag aatcctgtgg aatgaaataa accggtctat
     2041 ccgtctatta cccttttagc tgcgctgtat cgtcgccgta ttcccgcatt aaccatgacc
     2101 gtagcccgac ggggaattcc ttctgcgtga gtgtgcggga ataatcaaaa acgatgcaca
     2161 ccgggtttta ctgtgctgac agacgcaggg ttaccctcat agtcgctttt ccggtgcgat
     2221 ggtggaagaa accgggatgt tcatccatca tcactctgga ttgatgtata tgctctcttt
     2281 tctgacgtta gtctccgacg gcaggcttca atgacccagg ctgagaaatt cccagaccct
     2341 ttttgctcaa gagcgatgtt aatttgttca atcatttggt taggaaagcg gatgttgcgg
     2401 gttgttgttc tgcgggtttt gttcttcgtt gacatgaggt tgccccgtat tcagtgtcgc
     2461 tgatttgtat tgtctgaagt tgtttttacg ttaagttgat gcagatcaat taatacgata
     2521 tctgcgtcat aattgattat ttgacgtggt ttgattgcgt agatgcacgt tgtgacatgc
     2581 agatgataat tattatcatt ttgcgggtcc tttccggcga tccgacaggt tacggggcgg
     2641 cgacctcgcg ggttttcgct atttatgaaa attttccggt ttaaggcgtt tccgttcttc
     2701 ttcgtcgtaa cttaatgttt ttatttaaaa taccccctga aaagaaagga aacgacaggt
     2761 gctgaaaacg aacttttggg cctttgtcgt ttcctttctc tgtttttggc cgtggaatga
     2821 acaatggaag tcaacaaaaa gcagctggct gacattttcg gtgcgagtat ccgtaccatt
     2881 cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg gtggcaaggg taatgaggtg
     2941 ctttatgact ctgccgccgt tataaaatgg tatgccgaaa gggatgctga aattgagaac
     3001 gaaaagctgc gccgggaggt tgaagaactg cgccaggcca gcgaggcaga tctccagcca
     3061 gggactattg agtacgaacg ccatcgactt acgcgtgcgc aggccgacgc acaggaactg
     3121 aagaatgcca gagactcagc tgaagtggtg gaaaccgcat tctgtacttt cgtgctgtcg
     3181 cggatcgcag gtgaaattgc cagtattctc gacgggatcc ccctgtcggt gcagcggcgt
     3241 tttccggaac tggaaaaccg acatgttgat ttcctgaaac gggatatcat caaagccatg
     3301 aacaaagcag ccgcgctgga tgaactgata ccggggttgc tgagtgaata tatcgaacag
     3361 tcaggttaac aggctgcggc attttgtccg cgccgggctt cgctcactgt tcaggccgga
     3421 gccacagacc gccgttgaat gggcggatgc caattactat ctcccgaaag aatccgcata
     3481 ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc atcatgaatg cgatgggcag
     3541 cgactacatc cgtgaggtga atgtggtgaa gtttgcccgt gtcggttatt ccaaaatgct
     3601 gttgggtgtt tatgcctact tcatagagca taagcagcgc aacaccctta tctggttgcc
     3661 gacggatggt gatgccgaga actttatgaa aacccacgtt gagccgacta ttcgtgatat
     3721 tccgtcgctg ctggcgctgg ccccgtggta tggcaaaaag caccgggata acacgctcac
     3781 catgaagcgt ttcactaatg ggcgtggctt ctggtgcctg ggcggtaaag cggcaaaaaa
     3841 ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa cttgctgctt ttgatgatga
     3901 tattgaacag gaaggctctc cgacgttcct gggtgacaag cgtattgaag gctcggtctg
     3961 gccaaagtcc atccgtggct ccacgcccaa agtgagaggc acctgtcaga ttgagcgtgc
     4021 agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc tgcccgcact gcggggagga
     4081 gcagtacctt aaatttggcg ataaagagac gccgtttggc ctcaaatgga cgccggatga
     4141 tccctccagc gtgttttatc tctgcgaaca taatgcctgc gtcatccgcc agcaggagct
     4201 ggacttcact gatgcccgtt atatctgcga aaagaccgag atctggaccc gtgatggcat
     4261 tctctggttt tcgtcatccg gtgaagagat tgagccgccg gacagcgtga cctttcacat
     4321 ctggacggcg tacagcccgt tcaccacctg ggtgcagatt gtcaaagact ggatgaaaac
     4381 gaaaggggat acgggaaaac gtaaaacctt cgtgaacacc acgctcggtg agacatggga
     4441 agcgaaaatc ggtgaacgtc cggatgctga agtgatggca gagcggaaag agcattattc
     4501 agcgcccgtt cctgaccgtg tggcttacct gactgccggt atcgactccc agctggaccg
     4561 ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa agctggctga ttgaccggca
     4621 gattattatg ggtcgccacg acgatgaaca gacgctgctg cgtgtggatg aggccatcaa
     4681 taaaacctat acccgccgga atggtgcaga aatgtcggta tcccgtatct gctgggatac
     4741 tggcgggatt gacccgacca ttgtgtatga acgctcgaaa aaacatgggc tgttccgggt
     4801 gatccccatt aaaggggcat ccgtctacgg aaagccggtg gccagcatgc cacgtaagcg
     4861 aaacaaaaac ggggtttacc ttaccgaaat cggtacggat accgcgaaag agcagattta
     4921 taaccgcttc acactgacgc cggaagggga tgaaccgctt cccggtgccg ttcacttccc
     4981 gaataacccg gatatttttg atctgaccga agcgcagcag ctgactgctg aagagcaggt
     5041 cgaaaaatgg gtggatggca ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga
     5101 ggcgctcgac tgcttcgttt atgcgctggc ggcgctgcgc atcagtattt cccgctggca
     5161 gctggatctc agtgcactgc tggcgagcct gcaggaagag gatggtgcag caaccaacaa
     5221 gaaaacactg gcagattacg cccgtgcctt atccggagag gatgaatgac gcgacaggaa
     5281 gaacttgccg ctgcccgtgc ggcactgcat gacctgatga caggtaaacg ggtggcaacg
     5341 gtacagaaag acggacggcg agtggagttt acggtcactt ccgtgtctga cctgaaaaaa
     5401 tacattgcgg agctggaggt gcagaccggc atgacacagc gacgcagggg acctgcagga
     5461 ttttatgtat gaaaacgccc accattccca cccttctggg accggacggc atgacatcgc
     5521 tgcgtgaata tgccggttat cacggcggtg gcagcggatt tggtgggcag ttgcgggcgt
     5581 ggaacccacc gggtgaaagt gtggatgcag ccctgctgcc caactttacc cgtggcaatg
     5641 cccgcgcgga cgatctggta cgcaataacg gctatgctgc caacgccatc cagctgcatc
     5701 aggatcatat cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg
     5761 gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt
     5821 ttgccgagga cgactgttgc tgcattgacg ttgagcgaaa acgcacgttc accatgatga
     5881 ttcgggaagg tgtggccatg catgccttta acggtgaact gttcgttcag gccacctggg
     5941 ataccagccc gtcgcgactg ttccggacac agttccggat ggtcagtccg aagcgtatca
     6001 gcaacccgaa caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca
     6061 gcggcgcggc gctgggatat tacgtcagcg aggacggcta tcctggctgg atgccgcaga
     6121 aatggacatg gataccccgt gagttacccg gcgggcgcgc ctcgttcatt cacgtttttg
     6181 aacccgtgga ggacggacag acccgcggtg caaatgtgtt ttacagcgtg atggagcaga
     6241 tgaagatgct cgacacgctg cagaacacgc agctgcagag cgccattgtg aaggcgatgt
     6301 atgccgccac cattgagagt gagctggata cgcagtcagc gatggatttt attctgggcg
     6361 cgaacagtca ggagcagcgg gaaaggctga ccggctggat tggtgaaatt gccgcgtatt
     6421 acgctgcagc gccggtccgg ctgggaggcg caaaagtacc gcacctgatg ccgggggact
     6481 cactgaacct gcagacggct caggacacgg ataacggcta ctccgtgttt gagcagtcac
     6541 tgttgcggta tatcgctgcc gggctgggtg tctcgtatga gcaactttcc cggaattacg
     6601 cccagatgag ctactccacg gcacgggcca gtgcgaacga gtcgtgggcg tactttatgg
     6661 ggcggcgaaa attcgtcgca tcccgtcagg cgagccagat gtttctgtgc tggctggaag
     6721 aggccatcgt tcgccgcgtg gtgacgttac cttcaaaagc gcgcttcagc tttcaggaag
     6781 cccgcagtgc ctgggggaac tgcgactgga taggctccgg tcgtatggcc atcgatggtc
     6841 tgaaagaagt tcaggaagcg gtgatgctga tagaagccgg actgagcacc tacgagaaag
     6901 agtgcgcgaa acgcggtgac gactatcagg aaatttttgc ccagcaggtc cgtgaaacga
     6961 tggagcgccg cgcagctggt cttaaaccgc ccgcctgggc ggctgcggca tttgaatccg
     7021 ggctgcgaca atcaacagag gaggagaaga gtgacagcag agctgcgtaa tctcccgcat
     7081 attgccagca tggcttttaa tgagccgctg atgcttgaac ccgcctatgc gcgggttttc
     7141 ttttgtgcgc ttgcaggcca gcttgggatc agccgcctga cggatgcggt gtccggcgac
     7201 agcctgactg cccaggaggc actcgcgacg ctggcattat ccggtgatga tgacggacca
     7261 cgacaggccc gcagttatca ggtcatgaac ggcatcgccg tgctgccggt gtccggcacg
     7321 ctggtcagcc ggacgcgggc gctgcagccg tactcgggga tgaccggtta caacggcatt
     7381 atcgcccgtc tgcaacaggc tgccagcgat ccgatggtgg acggcattct gctcgatatg
     7441 gacacgcccg gcgggatggt ggcgggggca tttgactgcg ctgacatcat cgcccgtgtg
     7501 cgtgacataa agccggtatg ggcgctggcc aatgacatga actgcagcgc aggtcagctg
     7561 cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg cccggacagg ctccatcggc
     7621 gtcatgatgg ctcacagtaa ttacggcgct gcactggaga aacagggcgt ggaaatcacg
     7681 ctgatttaca gcggcagcca taaggtggat ggcaacccct acagccatct accggatgat
     7741 gtccgggaaa cattgcagtc ccggatggat gcaacccgcc ggatgtttgc acagaaggtg
     7801 tcggcatata ccggcctgtc cgtgcaggct gtgctggata ccgaggctgc agtgtacagc
     7861 ggtcaggagg ccattgatgc cggactggct gatgaacttg tcaacagcac cgatgcgatc
     7921 accgttatgc gtgatgcact ggatgcacgt aaatcccgtc tctcaggagg gcgaatgacc
     7981 aaagagactc aatcaacaac tgtttcagcc actgcttcgc aggctgacgt tactgacgtg
     8041 gtgccagcga cggagggcga aaacgccagc gcggcgcagc cggacgtgaa cgcgcagatc
     8101 accgctgcgg ttgcggcaga aaacagccgc attatgggga tcctcaactg tgaggaggct
     8161 cacggacgcg aagaacaggc ccgcgtgctg gcagaaaccc ccggtatgac cgtggaaacg
     8221 gcccgccgca ttctggcagc tgcaccacag agtgcacagg cgcgcagtga cactgcgctg
     8281 gatcgtctga tgcagggggc accggcaccg ctggctgcag gtaacccggc atctgatgcc
     8341 gttaacgatt tgctgaacac accagtgtaa gggatgttta tgacgagcaa agaaaccttt
     8401 acccattacc agccgctggg caacagtgac ccggctcata ccgcaaccgc gcccggcgga
     8461 ttgagtgcga aagcgcctgc aatgaccccg ctgatgctgg acacatccac ccgtaagctg
     8521 gttgcgtggg atggcaccac cgacggtgct gccgttggca ttcttgcggt tgctgctgac
     8581 cagaccagca ccacactgac gttctacaag tccggcacgt tccgttatga ggatgtgctc
     8641 tggccggagg ctgccagcga cgagacgaaa aaacggaccg cgtttgccgg aacggcaatc
     8701 agcatcgttt aactttaccc ttcatcacta aaggccgcct gtgcggcttt ttttacggga
     8761 tttttttatg tcgatgtaca caaccgccca gctgctggcg gcaaatgagc agaaatttaa
     8821 gtttgatccg ctgtttctgc gtctcttttt ccgtgagagc tatcccttca ccacggagaa
     8881 agtctatctc tcacaaattc cgggactggt aaacatggcg ctgtacgttt cgccgattgt
     8941 ttccggtgag gttatccgct cccgtggcgg ctccacctct gaatttacac cgggatatgt
     9001 caagccgaag catgaggtga atccgcagat gaccctgcgt cgcctgccgg atgaagatcc
     9061 acagaatctg gcggacccgg cttaccgccg ccgtcgcatc atcatgcaga acatgcgaga
     9121 cgaagagctg gccattgctc aggtcgaaga gatgcaggcc gtttctgccg tgctcaaggg
     9181 caaatacacc atgaccggtg aagccttcga tccggttgag gtggatatgg gccgcagtgc
     9241 ggcgaacaac atcacgcagt ccggcggcac ggagtggagc aagcgtgaca agtccacgta
     9301 tgacccgacc gacgatatcg aagcctacgc gctgaacgcc agcggcgtgg tgaatatcat
     9361 cgtgttcgat ccgaaaggct gggcgctgtt ccgttccttc aaagccgtca aggagaagct
     9421 ggatacccgt cgcggctctc attccgagct ggagacagcg gtaaaagacc tgggcaaagc
     9481 ggtgtcctat aagggaatgt atggcgatgt ggccatcgtc gtgtattccg gacagtacgt
     9541 ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg atggtgctgg ggaacactca
     9601 ggctcgcggt ctgcgcacct atggctgcat tcaggatgcg gacgcacagc gcgaaggcat
     9661 taacgcctct gcccgttacc cgaaaaactg ggtgaccacc ggcgatccgg cgcgtgagtt
     9721 caccatgatt cagtcagcac cgctgatgct gctggctgac cctgatgagt tcgtttccgt
     9781 acaactggcg taatcgtggc ccttcggggc cattttctct ctgtggagga gttcatgacg
     9841 aaagatgaac tgattgcccg tctccggtcg ctgggtgagc aactgaaccg tgatgtcagc
     9901 ctgacgggga cgaaagaaga actggcgctc cgtgtggcag agctggaaga ggagcttgat
     9961 gacacggatg acgcagccgg tcaggacacg tctgtcagcc cggaaaatgc actgaccgga
    10021 catgaaaatg aggtggtatc agcacagacg gataccgtga ttcaggatac ggctgaactg
    10081 gtcacggtcg tggcactggt gacgctgcat actgatgcac ttcacgccac gcgggataaa
    10141 cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct ctgccggtgt ggcagccgaa
    10201 atgacagagc gcggcctggc cagaatgcaa taacgggagg cgctgtggct gatttcgata
    10261 acctgttcga tgctgccatt gcccgcgccg atgaaacgat acgcgggtac atgggaacgt
    10321 cagccaccat gacatccggt gagcagtcag gtgctgtgat acgtggtgtt tttgatgacc
    10381 ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga aggctccagc ccgtccctgt
    10441 ttgtccggac tgatgatgtg cggcagctgc ggcgcggcga cacgctgacc atcggtgagg
    10501 aaaacttctg gatagaccgg atttcgccgg atgatggcgg aagctgtcat ctctggcttg
    10561 ggcggggcgt accgcctgcc gttaaccgtc gccgctgaaa gggggatgta tggccataaa
    10621 aggtcttgag caggccgttg aaaacctcag ccgtatcagc agaacggcgg tgcccggtgc
    10681 cgccgcaatg gccattaacc gcgttgcttc atccgcgata tcgcagtcgg tggcacaggt
    10741 tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa agggccaggc tgaaaagggc
    10801 cacggtcaaa aatccgcagg ccagaatcag ggttagccgg ggggatttgc ccgtaataaa
    10861 gctgggtaac gcgcgggttg tcctgtcccg acgcaggcgt cgtaaaaagg ggcagcgttc
    10921 agccctgaaa ggtggcggca gtgtgcttgt ggtgggaaac cgtcgtattc ccggcgcgtt
    10981 tattcagcaa ctgaaaaatg gccggtggca tgtcatgcag cgtgtggccg ggaaaaaccg
    11041 ttaccccatt gatgtggtga aaatcccgat ggcggtgccg ctgaccacgg cgtttaaaca
    11101 gaatattgaa cggatacggc gtgaacgtct cccgaaagag ctgggctatg cgctgcagca
    11161 tcaactgaga atggtaataa agcgatgaaa catactgaac tccgtgcagc cgtactggat
    11221 gcactggaga agcatgacac cggggcgacg ctttttgatg gtcgccccgc tgtttttgat
    11281 gaggaagatt ttccggcaat tgccgtttat ctcaccggcg ctgaatacac gggcgaagag
    11341 ctggacagcg atacctggca ggcggagctg catattgaag tttccctgcc tgctcaggtg
    11401 ccggattcag agctggattc gtggatggag tcccggattt atccggtgat gagcgatatc
    11461 ccggcactgt cagatttgat caccagtatg gtggccagtg gctatgacta ccggcgcgac
    11521 gatgatgcgg gcctgtggag ttcagccgat ctgacttatg tcattaccta tgaaatgtga
    11581 ggacgctatg cctgtaccaa atcctgtaat gccggtgaaa ggtgccggga ccaccctgtg
    11641 ggtttataag gggagcggtg acccttatgc gaacccgctt tcagacgttg actggtcgcg
    11701 tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
    11761 ctatctcgat gatgaagatg cagactggac tgcgaccggg caggggcaga aatctgccgg
    11821 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
    11881 gtggtttaat gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
    11941 tgtgttccgc ggctgggtca gcagtatcgg taaggcggtg acggcgaagg aagtgattac
    12001 ccgcacggtg aaagtcacca acgtaggacg tccgtcgatg gcagaagatc gcagcacggt
    12061 aacagcggca accggcatga ccgtgacgcc tgccagcacc tcggtggtga aagggcagag
    12121 cacgacgctg accgtggcat tccagccgga aggcgcaacc gacaagagct tccgtgcggt
    12181 gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg
    12241 tgttgctgca ggcaaggtca acattccggt cgtatccggt aatggtgaac ttgctgcggt
    12301 tgcagaaatc aacgtcaccg ccagttaatc cggagagtca gcgatgttcc tgaaaaccga
    12361 atcatttgaa cataacggtg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat
    12421 tgagcatctc gccctgatga aacgacaggc agaacaggcg gagtcagaca gcaaccggaa
    12481 gtttactgtg gaagacgcca tcagaaccgg cgcttttgtg gtggcgatgt ccctgtggca
    12541 taaccatccg cagaagacaa agctgccttc tatgaatgaa gccgttaaac agattgagca
    12601 ggaagtgctt accacctggc ccacggaggc aatttctcat gctgaaaacg tggtgtaccg
    12661 gctgtccggt atgtatgagt ttgtggtgaa tgatgctcct gaacagacag aggacgccgg
    12721 gcctgcagag cctgtttctg cgggaaagtg ttcgatggtg agctgagttt tgccctgaaa
    12781 ctggcgcgag agatggggcg acccgactgg cgtgccatgc ttgccgggat gtcatccacg
    12841 gagtatgccg actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat
    12901 atgcactttt ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggatatg
    12961 catccgctgg atttcagtct gctgaaccgg cgtgaggctg acgaagagcc tgaagatgat
    13021 gtgctgatgc agaaagcggc agggcttgcc ggaggcgtcc gctttggccc ggatgtgaat
    13081 gaagttatcc ccgtttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
    13141 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
    13201 cgttgatttg agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
    13261 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag
    13321 ccgacaggca ctggctgcac agaaagcggg gatttccgtc gggcagtata aagctgccat
    13381 gcgtatgctg cctgcgcagt tcactgacgt ggccacgcag cttgcaggcg ggcaaagtcc
    13441 gtggctgatc ctgctgcaac agggtggtca ggtgaaggac tccttcggcg ggatgatccc
    13501 catgttcagg gggcttgccg gtgcgctcac cctgccgatg gtcggggcca cctcgctggc
    13561 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    13621 caacaaaacg ctggtccttt ccggcaatca gtcggggctg acggcagatc gcatgctggt
    13681 cctgtccaga gccgggcagg cggcagggct gacgtttgac cagaccagcg agtcactcag
    13741 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    13801 ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    13861 gctgaccaca gacccgacgt cagggctgac agcgatggca cgccagttcc ataacgtgac
    13921 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgagg ccggggcatt
    13981 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    14041 gaacatgggc acgctggaga cctgggcaga caggacagcg cgggcattca aatccatgtg
    14101 ggatgcggtg ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    14161 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt attttgttaa
    14221 cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc
    14281 agcgagaaag aaggctgagc agcagactca acaggacaaa aatgcgcagc agcagagcga
    14341 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    14401 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    14461 gaaaatcctg caggcggatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    14521 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga
    14581 cagtgctcat gctgccctgc tgatgcttca gacagaactc cggacgctgg agaagcatgc
    14641 cggagcgaat gagaaaatca gccagcagcg ccgggatttg tggaaggcag aaagtcagtt
    14701 cgcggtactg gaggaggcgg cacaacgtcg ccagctgtcc gcacaggaga aatccctgct
    14761 ggcgcataaa gatgagacgc tggagtacaa acgccagctg gctgcacttg gcgacaaggt
    14821 tacgtatcag gagcacctga atgcgctggc gcagcaggcg gataagttcg cacagcaaca
    14881 acgggcaaaa cgggcagcca ttgaggcgaa aaaccggggg cttactgacc ggcaggcagc
    14941 gcgggaagcc acagaacagc gcctgaagga acagtatggc gataatccgc tggcgctgaa
    15001 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggagctg
    15061 gatggcaggc ctcaggtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    15121 gcaggttaaa agtgcagcca cgcagacctt tgatggtatt gcgcagaata tggcggcgat
    15181 gctgaccggc agtgaacaga actggcgcag cttcacccgc tccgtgctgt ccatgatgac
    15241 agaaattctg cttaagcagg caatggtggg gattgtcggg agtatcggca gcgccattgg
    15301 cggggctgtt ggtggcggcg catcagcgtc aggcggtaca gccattcagg cagctgcggc
    15361 gaaattccat tttgcgaccg gaggatttac gggaaccggc ggcaaatatg agccagcggg
    15421 gattgttcac cgtggtgaat ttgtcttcac taaggaggca accagccgga ttggcgtggg
    15481 gaatctctac cggctgatgc gcggctatgc caccggcggt tatgtcggta caccgggcag
    15541 catggcggac agccgctcgc aggcgtccgg gaagtttgag cagaataacc atgtggtgat
    15601 taacaacgac ggcacgaacg gtcagatagg gccacaggcg ctgaaggcgg tttatgacgt
    15661 agcccgtaag gcggcaatgg atgttgtgac cgggcagatg cgcgatggtg gtctgttctc
    15721 cggaggtgga cgatgaaaac cttccgctgg aaagtgaaac ccggtatgga tgtggcttcg
    15781 gccccttccg taagaaaggt gcgctttggt gatggctatt ctcagcgagc gcctgccggg
    15841 ctgaatgccg acctgaaaac gtacagcgtg acgctttctg ttccccgttg ggaggccacg
    15901 gcgcttgagt cgtttctggc tgagcacggg ggctggaaag cctttctgtg gacgccgcct
    15961 tatgagtggc ggcagataaa ggtgacctgc gcaaaatggt cgtcgcgggt cagtatgctg
    16021 cgtgttgagt tcagcgcaga gtttgaacag gtggtgaact gatgcaggat atccggcagg
    16081 aaacactgaa tgaatgcacc cgtgcggagc agtcggccag cgtggtgctc tgggaaatcg
    16141 acctgacaga ggtcggtgga gaacgttatt ttttctgtaa tgagcagaac gaaaaaggtg
    16201 agccggtcac ctggcagggg cgacagtatc agccgtatcc cattcagggg agtggttttg
    16261 aactgaatgg caaaggcacc agtacgcgcc ccacgctgac ggtttctaac ctgtacggta
    16321 tggtcaccgg gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta
    16381 aggtttacgc ccgttttctg gatgcggtga acttcgtcaa cggaaacagt gacgccgatc
    16441 cggagcagga ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtga
    16501 gtgcctcctt tgtactgtcc acgccgacgg aaacagatgg cgctgttttt ccgggacgta
    16561 tcatgctggc caacacctgc acctggacct atcgcggcga tgagtgcggt tatcacggtc
    16621 cggcggtcgc ggatgaatat gaccagccaa cgtccgatat cacgaaggat aaatgcagca
    16681 aatgcctgag tggctgtaag tttcgcaata acgtcggcaa ctttggcggc ttcctttcca
    16741 ttaacaaact ttcgcagtaa atcccatgac acagacagaa tcagcgattc tggcgcacgc
    16801 ccggcgatgt gcgccagcgg agtcgtgcgg cttcgtggta agcacgccgg agggggaaag
    16861 atatttcccc tgcgtgaata tctccggtga gccggaggcg tatttccgta tgtcgccgga
    16921 ggactggctg caggcagaaa tgcagggtga gattgtggca ctggtccaca gccaccccgg
    16981 tggtctgccc tggctgagtg aggccgaccg gcggctgcag gtgcagagtg atttgccgtg
    17041 gtggctggtc tgccggggga cgattcataa gttccgctgt gtgccgcatc tcaccgggcg
    17101 gcgttttgag cacggggtga cggactgtta cacgctgttc cgggatgctt atcatctggc
    17161 ggggattgag atgccggatt ttcatcgcgg ggatgactgg tggcgtcacg gtcagaatct
    17221 ctatcttgac aatatggagg cgactggttt ttaccgtgtc gcactgacag aggcgcagcc
    17281 tggcgacgtg ctgctgtgct gctttggttc atcggtgccg aatcatgccg ccatttactg
    17341 tggtgacggc gagctgctgc accatattcc tgaacaactg agcaaacgag agaggtatac
    17401 cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggcatggc acgcatctgc
    17461 ctttacgggg atttacaacg atttggccgc cgcatcgacc ttcgtgtgaa aacgggggct
    17521 gaagccatcc gggcgctggc cacacagctt ccgtcgtttc gccagaaact gaatgagggc
    17581 tggtatcagg tgcgcatttc cgggcgtgat gcaggcgaaa atgaattatc tgcccgtctt
    17641 aatgaaccgc tggcaaatgg tgccgtgatc cacgtcgttc cgcgtctggc gggtgccaaa
    17701 agtggcggtg tgtttcaggc ggtgctgggt gcggcgctga ttgcggtggc atggtggaac
    17761 cctgttggct ggctgggggc cgcggctgta tcgggtctgt atgcagcagg ggccagtatg
    17821 atcctgggtg gtgtggcgca gatgctggca ccgaaagcca ggacgcccac ggcagccagt
    17881 acagataacg gcaaacagaa cacgtatttc tcgtcactgg ataacatggt tgcccagggc
    17941 aatgttctgc ctgttctgta cggggaaatg cgcgtgggat cacgtgtggt ttctcaggag
    18001 atcagcacgg cagacgaagg ggatggtggt caggttgtgg tgattggtcg ctgatgcaaa
    18061 acattttatg tgaaaccgcc tgcgggcggt tttgtcgttt atggagcgtg acgaatgggt
    18121 aaaggcagca gtaaggggca taccccgcgc gaagcgaagg acaacctgaa gtccacgcag
    18181 ctgctgagtg tgatcgatgc catcagcgaa gggccggttg aaggtccggt ggatggatta
    18241 aaaagcgtgc tgctgaacag tacgccggtg ctggacagtg aggggaatac caatatatcc
    18301 ggcgtcacgg tggtgttccg ggccggtgag caggagcaga caccgccgga gggatttgaa
    18361 tcctccggct ccgagacggt gctcggtaca gaagtgaaat atgacacgcc gatcacccgg
    18421 accatcacgt cggcaaacat tgaccgtctg cgttttactt tcggcgtgca ggcactggtg
    18481 gaaaccacct caaaggggga caggaatcca tcggaagtcc gcctgctggt tcagatacaa
    18541 cgtaacggtg gctgggtgac ggaaaaagac atcaccatta agggcaaaac cacctcgcag
    18601 tatctggcct cggtggtggt ggataacctg ccgccgcgcc cgtttaatat ccggatgcgc
    18661 agaatgacgc cggacagcac cacagaccag ctgcagaaca aaacgctctg gtcgtcatac
    18721 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cactggtcgg cgtgcaggtg
    18781 gattcggagc agttcggcag ccagcaggtg agccgtaatt atcatctgcg cgggcgtatt
    18841 ctgcaggtgc cgtcgaatta taacccgcag acgcggcaat acagcggtat ctgggacgga
    18901 acgtttaaac cggcatacag caacaacatg gcctggtgtc tgtgggatat gctgacccac
    18961 ccgcgctacg gcatggggaa acgtcttggt gcggcagatg tggataaatg ggcgctgtat
    19021 gtcatcggcc agaattgcga ccagtcggtg ccggacggct ttggcggcac ggagccgcgc
    19081 atcacctgta atgcgtacct gaccacacag cgcaaggcgt gggatgtgct cagtgatttc
    19141 tgctcggcga tgcgctgtat gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac
    19201 cgaccgtcgg ataaggtgtg gacctataac cgcagtaatg tggtgatgcc ggatgatggc
    19261 gcaccattcc gctacagctt cagcgccctg aaagaccgcc ataatgccgt tgaagtgaac
    19321 tggattgacc cggataacgg ctgggagacg gcaacagagc ttgtggagga ctcgcaggcc
    19381 attgcccgtt acggtcgtaa cgtcacgaag atggatgcgt ttggctgtac cagtcggggg
    19441 caggcgcacc gcgccgggct gtggctgatt aaaacagaac tgctggaaac gcagaccgtg
    19501 gacttcagcg tgggtgccga agggcttcgc catgtaccag gtgatgtcat tgaaatctgt
    19561 gatgatgact atgcggggat cagcaccggc gggcgcgtgc tggcggtgaa cagccagacg
    19621 cggacactga cgctcgaccg tgaaatcacg ctgccatcct ccggcaccac gctgataagc
    19681 ctggttgacg ggcagggtaa tccggtgagc gtggaggtcc agtccgtcac cgacggcgtg
    19741 aaggtgaaag tgagccgggt tcctgacggc gttgccgagt acagcgtgtg ggggctgaag
    19801 ctgccgacgc tgcgtcagcg cctgttccgc tgtgtgagta tccgtgagaa cgatgacggt
    19861 acgtatgcca tcactgccgt gcagcatgta ccggagaaag aagccatcgt ggataacggg
    19921 gcgcactttg acggtgacca gagcggcacg gtgaatggtg tcacgccgcc agcagttcag
    19981 cacctgaccg cagaagtcac cgcagacagc ggggaatacc aggtgctggc gcgctgggac
    20041 acgccgaagg tggtgaaggg ggtgagcttt atgcttcgcc tgaccgtggc agcggacgac
    20101 ggcagtgagc ggctggtcag cacggcccgg acgacggaaa ccacatatcg cttcacgcag
    20161 ctggcgctgg gaaattacag gctgacagtc cgggcggtaa atgcatgggg acagcagggc
    20221 gatccggcat cggtattgtt ccggatcgcc gcaccggcag cgccgtcgcg gattgagctg
    20281 acgccgggct attttcagat aaccgccacg ccgcatcttg cggtttatga tccgacggta
    20341 cagtttgagt tctggttctc ggaaaagcgg attgcggata tcaggcaggt tgaaaccaca
    20401 gcccgctatc ttggtacggc gctgtactgg atagccgcca gtatcaatat caaaccgggc
    20461 catgattatt acttttatat ccgcagtgtg aacaccgtag gcaaatcggc atttgtggag
    20521 gccgtcggtc gggcgagcga tgatgcatcc ggctatctgg attttttcaa aggcgagata
    20581 gggaaaaccc atctggctca ggagctgtgg acgcagattg ataacggtca gcttgcgcct
    20641 gacctggctg aaatcaggac gtccattacg gatgtcagca atgaaatcac gcagaccgtc
    20701 aataagaaac aggaagacca gagtgcggca attcagcaga tacagaaggt tcaggttgat
    20761 acaaataata acctgaacag catgtgggct gtgaagctgc agcagatgca ggacggacgc
    20821 ctttatatcg cgggtattgg tgctggtatt gagaacaccc ctgacggcat gcagagtcag
    20881 gtgctgctgg cggcggacag gattgcgatg gttaatcctg cgaatggcaa cacaaaaccg
    20941 atgtttgttg gtcagggcga tcagatattc atgaacgacg tgttcctgaa acgcctgacg
    21001 gcccccacca ttaccagcgg tggaaatcca ccggcatttt ccctgacacc ggacggaaag
    21061 ctgaccgcta aaaatgcgga tatcagtggc agtgtgaatg cgaacgccgg gacgctcaac
    21121 aatgtcacgg taaatgaaaa ctgtacgatt aagggcatgc tggaggcgac tcaggtcaga
    21181 ggtgacttcg ttaaagctgt atccaaatca tttccgaaac aggctggtac gtggggtaac
    21241 acggaaacac cagacgggac ggttacagtc accatcagcg atgatcataa ctttgaccgt
    21301 caaatcatta ttccgcccat tatctttaac ggaatagcgt atagcgatcc gggaagtggt
    21361 aataacccgg gaggtacaag atacacgggt tatggttttg aagttcgcaa aaacggtgta
    21421 ttaatcgcat ccagagaaac taaaggggcc attcccggta gctacagtgc ggttattgat
    21481 atgccgagtg gcaggggaag cgtcactctg gagtttaagg ttttccataa aggcaatcag
    21541 tgggcaggta atatcaccga ctgtacggtg attgtgacca aaaaagctgc ttccggcatc
    21601 agtattcgtt gaagttgtta taacccatat aagggcacca gaaatggtgc cttttttatt
    21661 gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc cgcagccatt
    21721 tgtctggccg tatccggtgc gcctgcatgg gcatctgaac atcagtccac gctgagcgcg
    21781 gggtatcttc atgcccggac caacgttccc ggcagcgatg atctgaacgg gattaacgtg
    21841 aaataccgtt atgagtttac ggacacactg gggctggtga cgtcattcag ctatgcagga
    21901 gacaagaatc gccagcttac ccgttacagc gatacccgct ggcatgaaga ttccgttcgt
    21961 aaccgctggt tcagcgt
//
LOCUS       DAPHJE010000074        21626 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPHJE010000074 DAPHJE010000000
VERSION     DAPHJE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.074"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            216..1730
                     /gene="caiT"
                     /locus_tag="RH222_002875"
     CDS             216..1730
                     /gene="caiT"
                     /locus_tag="RH222_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV3797864.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1761..2903
                     /gene="caiA"
                     /locus_tag="RH222_002876"
     CDS             1761..2903
                     /gene="caiA"
                     /locus_tag="RH222_002876"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV3797865.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3032..4249
                     /gene="caiB"
                     /locus_tag="RH222_002877"
     CDS             3032..4249
                     /gene="caiB"
                     /locus_tag="RH222_002877"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV3797866.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4323..5876
                     /gene="caiC"
                     /locus_tag="RH222_002878"
     CDS             4323..5876
                     /gene="caiC"
                     /locus_tag="RH222_002878"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV3797867.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5985..6770
                     /gene="caiD"
                     /locus_tag="RH222_002879"
     CDS             5985..6770
                     /gene="caiD"
                     /locus_tag="RH222_002879"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV3797868.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            6776..7366
                     /gene="caiE"
                     /locus_tag="RH222_002880"
     CDS             6776..7366
                     /gene="caiE"
                     /locus_tag="RH222_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV3797869.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7452..7847)
                     /gene="caiF"
                     /locus_tag="RH222_002881"
     CDS             complement(7452..7847)
                     /gene="caiF"
                     /locus_tag="RH222_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV3797870.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8108..11329)
                     /gene="carB"
                     /locus_tag="RH222_002882"
     CDS             complement(8108..11329)
                     /gene="carB"
                     /locus_tag="RH222_002882"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV3797871.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11347..12495)
                     /gene="carA"
                     /locus_tag="RH222_002883"
     CDS             complement(11347..12495)
                     /gene="carA"
                     /locus_tag="RH222_002883"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV3797872.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPDKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12951..13772)
                     /gene="dapB"
                     /locus_tag="RH222_002884"
     CDS             complement(12951..13772)
                     /gene="dapB"
                     /locus_tag="RH222_002884"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV3797873.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(13939..14853)
                     /gene="rihC"
                     /locus_tag="RH222_002885"
     CDS             complement(13939..14853)
                     /gene="rihC"
                     /locus_tag="RH222_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV3797874.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14919..15869)
                     /gene="ispH"
                     /locus_tag="RH222_002886"
                     /gene_synonym="lytB"
     CDS             complement(14919..15869)
                     /gene="ispH"
                     /locus_tag="RH222_002886"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV3797875.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCIGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15871..16320)
                     /gene="fkpB"
                     /locus_tag="RH222_002887"
     CDS             complement(15871..16320)
                     /gene="fkpB"
                     /locus_tag="RH222_002887"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3797876.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16445..16939)
                     /gene="lspA"
                     /locus_tag="RH222_002888"
     CDS             complement(16445..16939)
                     /gene="lspA"
                     /locus_tag="RH222_002888"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV3797877.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16939..19755)
                     /gene="ileS"
                     /locus_tag="RH222_002889"
     CDS             complement(16939..19755)
                     /gene="ileS"
                     /locus_tag="RH222_002889"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV3797878.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19798..20739)
                     /gene="ribF"
                     /locus_tag="RH222_002890"
     CDS             complement(19798..20739)
                     /gene="ribF"
                     /locus_tag="RH222_002890"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV3797879.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20747..20965)
                     /locus_tag="RH222_002891"
     CDS             complement(20747..20965)
                     /locus_tag="RH222_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV3797880.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21068..21331
                     /gene="rpsT"
                     /locus_tag="RH222_002892"
     CDS             21068..21331
                     /gene="rpsT"
                     /locus_tag="RH222_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV3797881.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5157 a         5817 c         5553 g         5099 t
ORIGIN      
        1 caaactgatt aataatcaaa attaacgaga aaagcgccct ctattcaccc gaaacaaaaa
       61 tgtgataaca atcacagaat gcagcttatt gaatacccat tatgagttag ccattaacgc
      121 gtccacgagg ttaataataa ttatattaaa tgttaacaaa aataaaacaa acgggaacgc
      181 aaaataaata ttcgttttca cagtggaatt aactcatgaa gaatgaaaag agaaaaacgg
      241 gaatagaacc gaaggttttc tttccgccgt taataatcgt cggcatactt tgttggctta
      301 cagtcagaga tctggatgca gcgaatgtcg ttattaatgc tgtattcagt tacgtcacca
      361 atgtatgggg atgggcattt gaatggtata tggtggtgat gcttttcggt tggttctggc
      421 tggtgtttgg cccgtatgcc aaaaagcgtt taggtaacga accacctgaa tttagtaccg
      481 ccagttggat ctttatgatg ttcgcctcct gtacgtctgc tgccgtactg ttctggggat
      541 cgattgagat ctactactac atctccaccc cgccgtttgg cttagaaccg aactcgacag
      601 gagcgaaaga gttggggctg gcttacagct tgttccactg gggacctctg ccgtgggcca
      661 cttacagctt cctttcagtc gccttcgctt acttcttctt tgtccgcaaa atggaagtga
      721 ttcgccccag ctcgacactg gtgccgctgg taggtgaaaa acacgccaaa gggttgttcg
      781 gcactatcgt cgacaacttc tatctcgtcg ccttgatctt cgcgatgggt accagtctgg
      841 gccttgccac gccgctggtg accgagtgta tgcaatggtt gtttggcatt ccgcataccc
      901 tgcaactgga cgctatcatc attacctgct ggattatcct caacgccatt tgcgtcgctt
      961 gcggtctgca aaaaggggta cgtatcgcca gtgacgtgcg tagttacctg agcttcctga
     1021 tgctgggttg ggtgttcatt gtcagcggtg ccagcttcat catgaactac ttcaccgatt
     1081 cggtggggat gttgctgatg tatctgccgc gcatgttgtt ctataccgat cccatcgcta
     1141 aaggcggctt cccgcagggc tggaccgtgt tctactgggc atggtgggtg atttatgcta
     1201 tccagatgag tatcttcctc gcccgcatct cccgtggtcg tactgtgcgt gaactgtgct
     1261 tcggcatggt gctggggctg acagcgtcaa cctggatcct gtggactgta ctcggtagta
     1321 acactctgct gttgatagat aaaaacatca tcaacattcc aaatctgatc gaacagtacg
     1381 gtgtggcgcg cgccatcatt gaaacctggg ccgctctgcc actcagcacc gccaccatgt
     1441 ggggcttctt catcctctgc tttattgcca ccgttacgct ggttaacgcc tgctcttata
     1501 ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaaccacct ctgctggtgc
     1561 gtatcggttg gtcaattctg gttggcatta tcggtattgt tctgctggcg ctcggcggcc
     1621 tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc gctgttcttc gtcaacatta
     1681 tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa ttaaccccca
     1741 aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa ctgtttgtcg
     1801 ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc gagtgcgacc
     1861 gtgacagcgt ctacccggaa cgttttgtca aagcactggc ggatatgggt atcgacagtc
     1921 tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc gccgccgtgt
     1981 ggatggagct gggacgtctg ggggcaccaa cctatgtgct gtaccagttg ccgggcgggt
     2041 tcaacacctt cctgcgcgaa ggcacacaag agcagatcga caagattatg gctttccgcg
     2101 gcaccggtaa gcagatgtgg aactcagcga ttaccgaacc gggcgcgggc tccgacgtgg
     2161 gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat ggtagtaagt
     2221 gttttattac cagcagcgcc tacaccccgt acatcgtggt gatggcgcgc gacggggctt
     2281 ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg ggcatcaaag
     2341 tgaccaaact tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc acctttgacg
     2401 acgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt aaccgcgtca
     2461 aagaagagtt cgatcatgaa cgtttcctgg tagctctcac caactacggt acggcgatgt
     2521 gcgcctttga agatgcggcg cgctacgcca accagcgcgt gcagtttggc gaggctattg
     2581 gccgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta aactccatga
     2641 aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc tctggcgatg
     2701 cagcgatgtg caaatacttc tgcgccaatg cggcatttga agtggtggat agcgcaatgc
     2761 aggtgctggg cggtgtcggg attgcgggca accaccgcat cagccgcttc tggcgtgacc
     2821 tgcgtgtaga ccgcgtctcc gggggatctg acgaaatgca gatcctgacg ctgggtcgcg
     2881 cggtgctgaa gcaataccgc taagtccgtg cgctgcctga tgcgacgcta atgcgtctta
     2941 tcaggcctac gggagcacga atgtaggccg gataaagcgt ttacgccgca tccggcagtc
     3001 atgcgctaca taaatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg
     3061 gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccgggc cgttcgccgg
     3121 gcaaatgttc gcagaatggg gtgcggaagt tatctggatc gagaacgtcg cctgggccga
     3181 caccattcgc gttcaaccga actacccgca gctctcccgc cgcaatttgc acgcgctgtc
     3241 gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga
     3301 tatcttcatc gaagccagca aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt
     3361 actgtggcag cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg
     3421 caccgaggag tacaccaatc ttccggccta taacaccatc gcccaggcct tcagtggtta
     3481 cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta
     3541 cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag cgcgtgaaac
     3601 cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta
     3661 cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga
     3721 tccctactac gccggttgcg gtctgtataa atgtgccgac ggctacatcg tgatggaact
     3781 ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcag
     3841 tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc
     3901 actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga
     3961 acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag
     4021 caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac
     4081 ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg
     4141 aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa
     4201 cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt
     4261 cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg
     4321 caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg gcggacgttt
     4381 acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt
     4441 atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc
     4501 gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt
     4561 tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg
     4621 aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaattct
     4681 atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga
     4741 cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg aaaaatcaac
     4801 aacctgccac cttgtgctat gcaccgccgc tattgactga cgatacggcg gaaattctct
     4861 tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct
     4921 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg
     4981 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
     5041 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtgcaga
     5101 agtaccgcgc caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtacagc
     5161 cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt
     5221 cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg
     5281 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cgacgctggc
     5341 cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc
     5401 cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
     5461 tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc
     5521 tacataccgg cgataccgga tactgcgacg aagagggctt tttttatttc gtcgatcgcc
     5581 gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca
     5641 tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg
     5701 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
     5761 ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca
     5821 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
     5881 aacagcaggg aactaccctg ccagctaata atcacgctat aaaacaatat gcattttttg
     5941 cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
     6001 atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa gcgaatgcta
     6061 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
     6121 aattacgtgt cgccattatt accggtgccg gagagaagtt cttttccgcg ggctgggatt
     6181 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggatttgcgg
     6241 gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac ggctatgcct
     6301 ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca
     6361 gcttcgccct gccggaagcc aagctgggca tcgttcctga cagcggcggc gtgctgcgtc
     6421 taccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg
     6481 gcacagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga
     6541 tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgccccgctg gcgattgcgg
     6601 cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaagcg tatcgctata
     6661 ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca ttcggaagat gccgttgaag
     6721 ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag
     6781 ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag
     6841 tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
     6901 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
     6961 cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca
     7021 cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt
     7081 gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa
     7141 agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg
     7201 cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
     7261 tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa
     7321 tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg
     7381 acctggttgc tggataagat gctacaacat cgcatctggc aagcaggacg cccggtatca
     7441 acgagataaa attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
     7501 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
     7561 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
     7621 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aacttcgcag
     7681 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
     7741 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
     7801 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
     7861 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
     7921 attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct
     7981 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc
     8041 tcatcagaga accgtattac ttattcgatc aaattagcgg atgaaaaata tccgccatga
     8101 cacgctatta tttgatctgc gcgtgcattt cctgcaccga aattactttt tcggtcgcgt
     8161 cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt
     8221 tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcgcga cggcctgaag
     8281 tggtgttgat gatgtaggta tattcgccat tcttgatgcg gtcctgaatg tgcggacggc
     8341 cttcatgcac cttgttcacc agacgcggat taatgcccgc ttcgcccagc acaatcgccg
     8401 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
     8461 cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg accgtgtttc ttcatggtgg
     8521 agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatca
     8581 cttccccggt ggagcgcatt tctggcccta acagcgggtc aacacccggg aatttattga
     8641 acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct
     8701 gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg
     8761 tggctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga
     8821 cttcgttgtt tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg
     8881 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg
     8941 gcagagaaca tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccatgatgc
     9001 cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct accgcgtcat
     9061 caaggaagtg atccagcaac actggcgcat cgttagacac gctgaccgcc gtctggaagt
     9121 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
     9181 acggacgtac caccagcggg tagccaatct ctttcgcctt ctctaccgcc atttcaatag
     9241 cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc
     9301 gttcacggtc ttctgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag
     9361 cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc
     9421 ctttcggctt ctcaatacgc acgatttcta gcacatcttc cagagttacc ggctcgaagt
     9481 agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc cgggttacag ttaaccataa
     9541 tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg tacacagcag tagtcgaatt
     9601 cgataccctg accgatacgg ttcgggccgc cgccgagaac catgattttt tcacggtcgg
     9661 tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg
     9721 cgaactctgc cgcacaggta tccacgcgct tataaaccgg gtgcaggtca tactggtcgc
     9781 gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt tgccagacgc gcatcggcaa
     9841 agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg
     9901 ctactttctc ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatat
     9961 tggtcaggtt gaacacgccg tccacagaca ggcccgcacg gaaggcatcg gcgatgtacc
    10021 agatacgctc tgcgccagca tctttcagtt cgcgacggat tttggttaac gcttccgggt
    10081 catcgaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt
    10141 tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct
    10201 gagtggtcag acggtcgtta gcaccagcga atttttcgaa gttgaagcga ggaattttag
    10261 taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt
    10321 tcatcagttc gtcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga
    10381 aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa
    10441 caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa
    10501 cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg
    10561 tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt atggatgccc atcgcatcaa
    10621 agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct
    10681 cgtactcttt ccagccgatc agcgactcat caatcagcag ctctttggtt ggggagagat
    10741 ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata ccgccgccgc
    10801 taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg
    10861 ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct
    10921 tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcggtcgcac
    10981 caatcatagt cacgccgaac tcttccaaca cgccctgacg ctccagctcc agcgcgcagt
    11041 tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct ttttcaataa
    11101 tcttgcgtac tacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt
    11161 cggtcatgat ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca
    11221 gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg
    11281 ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac
    11341 tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa
    11401 cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga ccctggaagc tgaacgccgg
    11461 tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg
    11521 caggtttgca ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat
    11581 caccacgttt ttctccacat ctttatccgg atggttgccg ccgtggtgac caaatttcat
    11641 tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc agatgccgaa
    11701 taccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc
    11761 cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc
    11821 cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag
    11881 gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt
    11941 tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc
    12001 tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt
    12061 ttctaacgcc aacgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc
    12121 tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt
    12181 atggcgcttc aggtaagaag aaaggtcttc ggtattacgg aagttgctgg caatcagcgg
    12241 cagatcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt
    12301 agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg
    12361 atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc
    12421 cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag
    12481 cgctgactta atcaaaacac cctccagaga atattcactc actttatttg catattaatt
    12541 cacaatgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg
    12601 cattctggag atatgggcgc taaaagtcaa cttaatggcg ggatttttta tattattttg
    12661 tggctcatgc atgttttaca ggcattaagc gacaaaaaga tcatataacc tgttctggaa
    12721 aacgcttgcg cagcaagaag attgcatgaa ataaaagcga gcagggaaaa gtggaccaaa
    12781 tggtcaaaat ttacattaaa acaacaaaaa ccgaagacaa aacatgtaat ttttggcact
    12841 tatagacaat ttaaaaacat aacactaaaa ataaaagggc caaaaattaa agccctttat
    12901 aatcaataaa ttaaatgtgt tattcttgca ccataacaaa tattttgtgg ttacaaacta
    12961 ttgagatcaa gcacatctcg catatcaaaa agaccacttt ccttaccact caaccacaaa
    13021 gccgatctta ccgcgccgtt agcaaatgtc atgcggctgg acgccttatg ggtgatctcc
    13081 agacgctcgc caatatcggc aaacatcgcg gtatgttcgc caacgatgtc acctgcacgc
    13141 acggtggcaa aaccaatggt gccaggcaca cgttcaccgg tgtggccttc acgactgtag
    13201 accgcgcaat ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt
    13261 gcggtgcctg acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcgatatcg
    13321 gtgtagtcac ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca
    13381 acgctaaaat tggcggcaaa gacaatcgca atatcggcgg cggcgtcacg aatcgcctgt
    13441 ttaccggctt cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctggcgacaa
    13501 aaagcgagat ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca
    13561 tcttttaccg catcgaggct gctttgcacg gtaacgcctg ttttccctgc accagccagc
    13621 tcaccggcgt cgctgcccag taaagaagat ccttcgcgct ccagcgccgc gcccagctgc
    13681 acgccttcta atgccagcgc cgcctgaatc aactggcggc ccatacgccc cccggctccc
    13741 gcgatggcaa cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag
    13801 accgttttca gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga
    13861 aattaatgat aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg
    13921 ccgataacat gtgacaggtt acgacgccag agccagcacc tcagccaccc actgctggaa
    13981 gcctttcaca tccagatcca atgccacctt tacattggct ggtttgccca ggcaaccgtc
    14041 gatatcaacc accgtcgtgc ctgaggtaaa ttcgccttga gtttctaccg cgacaaaaca
    14101 cggcttgagg gtgaataagt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat
    14161 tcgcagaccg ctttgcatac tgccgctacg gtagtggcta aacagtgcat gaagcatttt
    14221 tccggtgcga ttcaactccg gcaaagtagc gagatagtca ggggttaata ttgcctgatt
    14281 ggtgacatcc agaccgcaca tgacgatttc gattccgcta cggaagacac aggcagccgc
    14341 ttctggatcg gcagcgatat taaactcggc gtttggcgta cagttgccgc gtccggcaga
    14401 accacccatg atcaccagac ggcgaatata cggcttgcat tccgggcatt gtgaaagtaa
    14461 cagcgcaata ttggttaacg ggccaatggc caccagggta acaggctctg gtgcacgcat
    14521 cagggcatcc cgaatcgcca gaaacgccgg tatcccgagc ggctttcggt tgtgctcaac
    14581 aaagtcgtag ccagccattc ccgattcgcc gtgcacagat gccgcatcac gcggtgcgcg
    14641 taccagcggc acagcggccc cttgggcgag cggaatctcc gcattccaga aatgcagcag
    14701 ttgcagggca ttgcgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag
    14761 ttgcaggtcg agttcgggtg caaaaatcgc ggcggcaatg gcgacggcat cgtcaatgcc
    14821 ggggtcagta tcgaggaaga taggtaaacg catgttttct ccataaaaaa tgccggtaac
    14881 aagaccggca ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg
    14941 acacgcagct ctttcggcac ttcgaaaaca atgttttctt cacgtccttc cagcggaatg
    15001 gcttcaccac cgcccagctg ttgcaaacgt gccaccacat tctgcaccag aatatccgga
    15061 gccgatgcgc ccgcagtcac gccgatgcat ttaacctctt tcacccactc ttcctggata
    15121 tctttcgcat catcaatcaa aaacgcgctt ttgcccatac gctgggccag ctccgccaga
    15181 cggttggagt tagaggagtt tttcgaaccg accaccagca caacttccgc ctgttcggcc
    15241 agggcacgta ccgcttcctg tcggttagtc gtggcgtagc agatgtcatc tttgcgcgga
    15301 ccgacaattt tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatccacc
    15361 gacagcgtgg tctgggtcat aaaggagagt ttctcttcgt ttttgaccgt cagtttccac
    15421 acatcgtctg gtgattcaac cagatacatt cccccttccg ggttgctgta ctgacccatc
    15481 gtgccttcca cttccgggtg cccggcgtga ccgatgagaa tagattcttc gccacggcga
    15541 ctagcgcggg cgacttccat atgcactttg gtcaccagcg gacaggtggc atcaaacacc
    15601 gtcaaatcgc ggctttttgc ttcgttacgt accgcctgag aaacaccgtg tgcggagaaa
    15661 atcaggatcg cgccgtccgg tacttcgcta atctgctcaa taaagatagc cccacgctca
    15721 cgcaagctat cgaccacata gcggttatgt accacttcgt gacggacata tatcggtgcg
    15781 ccgtaaatgg ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa
    15841 ccacgcgggt tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag
    15901 cacttcaata tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat caacggtaat
    15961 ggagtcgccg ttaatttcgc ggatcacgcc aggcatctca ctgccatcca ttgcggtaaa
    16021 aagcatgatt gcgccaattt ctggctcgcc tgcatccata aattcacggc gggagaagta
    16081 ctgaatcagg tccggtgacg gcacgccaaa cgccgcatct ggctccaacg agaaggtggt
    16141 tttatcgccc actttcagcc ccaacaggtg ttgctccagc ccttcagaaa gagaagcatc
    16201 acccaggcgg aacagcgccg gtttaccgtt gttgcgggta gactcggcgg tggtgccatc
    16261 gtcgagtttt agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat
    16321 gcaggtttgc tcttatattt aaggattttt gccggatgcg gcgtaaacgc cttatccggc
    16381 ctacgatttc gcagcaatct gtaggccgga taagatgcgt cagcatcgca tccggcaggg
    16441 tttattattg ttttttcgct ttagaaggta aaaaaccttc cagcacaatc agtgccgcac
    16501 cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa gtgccagtcg ccgacgtaga
    16561 agtcgatcat atcgacaacg aagccgtgcc acaggcggtc gaacaggttg cccagcgcgc
    16621 cgccaataat cagcgcgtaa gcgatattgt ttagcttctg cgtggccttc gagcgataca
    16681 tcatcactgc caggatcacg ctaataccaa tcgcaatacc ggcaaagaac caacgctgcc
    16741 aaccgccgct atcggcaagg aaactaaacg ccgcgccata gttacgcgca taatgcagat
    16801 taagcgacgg gaacagcggg accgtatccc ccagagcaaa gttctggagg atcaggtatt
    16861 tgctgcccag atcgataatc agcacgacta ccaccagcca cagccagcgt agccctgttg
    16921 aacagatcga ttgactcatc aggcaaactt acgtttttca ccgtcaccgg cgacgttgct
    16981 gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg ccgacatcct gggtgtagtg
    17041 ccagcagcgt gggcacttct caccttcggc tttactcaac gcgactttca gccctttgag
    17101 tacttcgctc tgctgagcat cagcaggtgc gtcgttatag tctgcaacgg tagcgccgga
    17161 ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc agtttcgccg acagttccgg
    17221 ttctgcatac aaggttactg ccgcttccag cgagccaccc actttcttgt cggcacgcgc
    17281 ttgctcaatg actttgttca cttcgccacg cactttcaac agctcgtccc agaacgcatc
    17341 gttcatcgct tcactgtctg ccaggccaaa caggccttcg taccactcac cggtgaagac
    17401 gtatttttca cgttcgcccg gcaggtagcc ccacacttca tcagcggtga aggagaggat
    17461 tggtgccatc cagcgcacca gcgcttctgc gatgtgatac agcgcagtct ggcagctacg
    17521 acgcgccaca ctgtccgctt tggcggtgta ctgacggtct ttgatgatgt cgaggtagaa
    17581 ggaacccatc tcaacggagc agaagcgcat cagacgctgt accacttcgt ggaaatcgta
    17641 tgcttcgtac gccttgagga tgtcttcctg tgccgctttc gcacaaccta cggcccagcg
    17701 atccagtacc accatctctt ccggtttcac catatctttt gctggatcaa aaccgttcag
    17761 gtttgccagc aggaagcgcg cggtgttacg gatacgacga tagctatcgg cagcacgttt
    17821 caggatctcg tcagaaacgg ccatttcacc ggtgtagtcg gttgatgcca cccacagacg
    17881 cagaatatcc gcgcccagtt tgttcatcac atcctgcggc gaaacggtat tgccgatgga
    17941 tttagacatc ttgcggccct gaccatccac ggtaaagccg tgggtcagta cctgacgata
    18001 cggcgctttg cctttcatcg cggtggagat cattagggaa gacatgaacc aaccacggtg
    18061 ctggtcagaa ccttccagat acatgtccgc tgcgtgaccg gcaaattccg gacgcacgtc
    18121 aacaacagaa gagtgggtag atccggagtc aaaccataca tccaatgtgt ccggcacttt
    18181 cacgtactga tcagcttcat cgccgaggat ctctttcgca tcaagatccc accacgcctg
    18241 gatgccgtca acttcaacgc gttttgccac ttcttccatt agttcgagag tacgcggatg
    18301 cagctcttcc gtgtctttgt gcacgaacag tgacatcggt acgccccagg tgcgctgacg
    18361 ggagatacac cagtcaggac ggttagcaac catcgactcg atacgcgcct ggccccagtc
    18421 cgggatccac tgcacgcctt tgatctcttt cagtgactgc gcacgcagac ctttctgatc
    18481 catgctgacg aaccactgcg gcgtcgcgcg gaagatgatc ggcgttttgt gacgccagca
    18541 gcacggatag ctgtgctgca ttttctcaac gtgcagcagc gcgccttttt cctgcagcag
    18601 cgcaacgacg atgtcgttcg ctttgaagac gttcacgcca tccagcgtcg gataagtgcc
    18661 cggcagataa gtgccgtccg ggccaaccgg gttagcggtt tccaggccgt atttctgacc
    18721 gatcacatag tcgtccgggc cgtggccagg cgcggtgtga acggcaccgg taccggcatc
    18781 cagggtaacg tgatcgccga ggattgccgg aacgtcgaag cccataaacg gatgggtaaa
    18841 gcgcagcaac tcaagctccg caccttttac cgtgccgaga atggtgtaat cggtcacgcc
    18901 gatacgctgc attacgcttt caaccagatc tttcgccaga atcacggcct gaccgtcgat
    18961 ctgcaccagc gcatagtcga aatcaggtgc aatagagatt gcgcggttcg caggcagagt
    19021 ccacggcgta gtggtccaga tcaccagcga gattgggccg ttaacgttgc ttacggcaaa
    19081 ttttgctttc agtgcatcct gatcgaccgc ctgaaaagca acgtcgatgg acggagaagt
    19141 tttgtcgtaa tactcaactt ccgcttccgc cagcgcagaa cggcagtcaa cgcaccagtg
    19201 taccggcttc gcgcctttgt gcaggtgacc gttgccgatg attttgccca gcgcgcggat
    19261 gatgttggct tcagttttga agtccatggt caggtacggg tgcgaccagt cgcccagcac
    19321 gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg gtcgccgcgt attcgcggca
    19381 cttggcgcgg aactcggcgg cggtgaattt ctcacccggc ttaccgtatt cttgctctac
    19441 tttcagctcg atcggcagac cgtggcagtc ccagccaggc acatacggcg agtcataacc
    19501 ggaaagccct ttggacttca cgataatgtc tttcagaatc ttgttaaccg agtgaccaat
    19561 atgaatgctg ccattcgcat aaggagggcc atcatgcaga atgaaggttt ttttgccttt
    19621 tttagccgca cggatgatgc cgtacagatc atcatcagtc caacgcgcca gcattcccgg
    19681 ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt tccggcaaat tcagggttga
    19741 tttatagtca ctcatcagat tctcggttcc gtgtttcggt ttgattacat aacaggctta
    19801 agccggtttt gttagcccaa aaaattcgcg ggcggttaat tcatcacgcg caatctgcgc
    19861 tttcagttcg tccagcgacg caaatcgctg ctcattgcgt atttttttac gcagcactac
    19921 ttgtatatgg cgaccgtaaa ggtccattgc aacatctaac aaatgcactt ccagctgctg
    19981 gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgcc acgccgggta acggcttttc
    20041 accgaggccc agcacttcta ccgcataaac ccctttcacc ggggaaacct gacggcgtag
    20101 cggtacattc gccgtcggga aacctatagt gcgccctaat tcatcaccgt ggactacacg
    20161 cccggagatg gcaaacgggt gccccagtaa actttctgcc agagccagat tgtcatccgc
    20221 cagcgcctga cgcacagcgg tgctgctaat acgcacgcca ccttcgcaaa aagtttgcgt
    20281 actagtgata tcgaagccgt attccatgcc agctttctgt aataacaaga aatcgccttc
    20341 acgaccagcg ccaaagcgga aatcatcccc tacggcaaga aattttacgc gcaaatgctt
    20401 caccagaaga tcgctgatga aattttgcgc ggttaacgcc gcgaaacgcc tgtcgaaacg
    20461 cacgcacagc acgtaatcaa cgccacactc tgcaaggtaa cgcagttttt cccgcagccg
    20521 ggtcagtctt gccggggctt tatcggtagc aaacagttcc agtggttgag gttcaaaaag
    20581 catcaccatc accggtaagt tgcgcttgcg cccttcttcc tgcaagccct gtaacagcgc
    20641 gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc agcacacacc cttcttgcgg
    20701 ggcctggctg agattatgta tgccgcgtat cagcttcatg tctggctcaa aacagtgaaa
    20761 atcgtccgag tataccttgt acagcggtca aggttaaccg gcgattgagt accgaaatca
    20821 gaaagaagtg gcttttcatt gccatggcgc aaatcacggg aagaaagtga ccgcctgctg
    20881 caatttttat cgcggaaaag ctgtattcac accccgcaag ctggtagaat cctgcgccat
    20941 cactacgtaa cgagtgccgg cacattaacg gcgcttattt gcacaaatcc attgacaaaa
    21001 gaaggctaaa agggcatatt cctcggcctt tgaattgtcc atatagaaca catttgggag
    21061 ttggaccttg gctaatatca aatcagctaa gaagcgcgcc attcagtctg aaaaggctcg
    21121 taagcacaac gcaagccgtc gctctatgat gcgtactttc atcaagaaag tatacgcagc
    21181 tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt aacgaaatgc aaccgatcgt
    21241 ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa gctgcacgtc ataaggctaa
    21301 cctgactgca cagatcaaca aactggctta atcgccaatt tgctgaagct ttgtgaaaaa
    21361 gcccgcgcaa gcgggttttt ttatgcccgc tgcttttatg atgcgtgaga gaacacacta
    21421 ccggttgcca gatagtcaaa agatgctggc ttttgcagac atgccgttac actttctgaa
    21481 ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca agaatatcta
    21541 ctcaatctcc agactcccct acaaagaatg tggatattgt gatacacatt gaggtattaa
    21601 acttaagacc actctatttt tgaatt
//
LOCUS       DAPHJE010000075        21616 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPHJE010000075 DAPHJE010000000
VERSION     DAPHJE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.075"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2922
                     /gene="ehaG"
                     /locus_tag="RH222_002893"
     CDS             <1..2922
                     /gene="ehaG"
                     /locus_tag="RH222_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HDV3797882.1"
                     /translation="FTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATN
                     TTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDGTVTAT
                     SSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSS
                     FSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSY
                     VVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGA
                     FSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTI
                     TAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGS
                     ISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLA
                     FNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRD
                     TSITENGVVIGYNTTDGELLGALSIGDDGKYRQIINVADDSEAHDAVTVRQLQNAIGA
                     VATTPSKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAI
                     GSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVA
                     AGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGST
                     KYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRR
                     ITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVS
                     AGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQ
                     AYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQ
                     W"
     gene            3290..4945
                     /gene="lldP"
                     /locus_tag="RH222_002894"
     CDS             3290..4945
                     /gene="lldP"
                     /locus_tag="RH222_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV3797883.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4945..5721
                     /gene="lldR"
                     /locus_tag="RH222_002895"
     CDS             4945..5721
                     /gene="lldR"
                     /locus_tag="RH222_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV3797884.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPSDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            5718..6908
                     /gene="lldD"
                     /locus_tag="RH222_002896"
     CDS             5718..6908
                     /gene="lldD"
                     /locus_tag="RH222_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV3797885.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            7094..7567
                     /gene="trmL"
                     /locus_tag="RH222_002897"
     CDS             7094..7567
                     /gene="trmL"
                     /locus_tag="RH222_002897"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV3797886.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7620..8441)
                     /gene="cysE"
                     /locus_tag="RH222_002898"
     CDS             complement(7620..8441)
                     /gene="cysE"
                     /locus_tag="RH222_002898"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV3797887.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8521..9540)
                     /gene="gpsA"
                     /locus_tag="RH222_002899"
     CDS             complement(8521..9540)
                     /gene="gpsA"
                     /locus_tag="RH222_002899"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV3797888.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9540..10007)
                     /gene="secB"
                     /locus_tag="RH222_002900"
     CDS             complement(9540..10007)
                     /gene="secB"
                     /locus_tag="RH222_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV3797889.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10070..10321)
                     /gene="grxC"
                     /locus_tag="RH222_002901"
     CDS             complement(10070..10321)
                     /gene="grxC"
                     /locus_tag="RH222_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV3797890.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10463..10894)
                     /locus_tag="RH222_002902"
     CDS             complement(10463..10894)
                     /locus_tag="RH222_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV3797891.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11139..12683
                     /gene="gpmM"
                     /locus_tag="RH222_002903"
                     /gene_synonym="pgmI"
     CDS             11139..12683
                     /gene="gpmM"
                     /locus_tag="RH222_002903"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV3797892.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12693..13976
                     /gene="envC"
                     /locus_tag="RH222_002904"
     CDS             12693..13976
                     /gene="envC"
                     /locus_tag="RH222_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV3797893.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13980..14939
                     /locus_tag="RH222_002905"
     CDS             13980..14939
                     /locus_tag="RH222_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV3797894.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(14926..15960)
                     /gene="waaH"
                     /locus_tag="RH222_002906"
     CDS             complement(14926..15960)
                     /gene="waaH"
                     /locus_tag="RH222_002906"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV3797895.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16199..17224)
                     /gene="tdh"
                     /locus_tag="RH222_002907"
     CDS             complement(16199..17224)
                     /gene="tdh"
                     /locus_tag="RH222_002907"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV3797896.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17234..18430)
                     /gene="kbl"
                     /locus_tag="RH222_002908"
     CDS             complement(17234..18430)
                     /gene="kbl"
                     /locus_tag="RH222_002908"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV3797897.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18644..19576
                     /gene="rfaD"
                     /locus_tag="RH222_002909"
     CDS             18644..19576
                     /gene="rfaD"
                     /locus_tag="RH222_002909"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV3797898.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            19586..20632
                     /gene="rfaF"
                     /locus_tag="RH222_002910"
     CDS             19586..20632
                     /gene="rfaF"
                     /locus_tag="RH222_002910"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV3797899.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALFGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20636..21607
                     /gene="rfaC"
                     /locus_tag="RH222_002911"
     CDS             20636..21607
                     /gene="rfaC"
                     /locus_tag="RH222_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV3797900.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
BASE COUNT         5267 a         5715 c         5661 g         4973 t
ORIGIN      
        1 ttcacggcag ctcatggcac cgagaccacc agcaaaatca ccaacgttaa agatggcgac
       61 ctgacgactg gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc
      121 gtggcgacga ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag
      181 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact
      241 gcagctcatg gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact
      301 gcaaccagtt ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc
      361 acctacttcg gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac
      421 aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca
      481 tttagcacgt ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc
      541 aaacacggta ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca
      601 gactccagtt ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt
      661 gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac
      721 accattgcta atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact
      781 cttgacgacg ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct
      841 cacggaaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct
      901 gccagcagcg acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat
      961 gcgctgggtg gtgacgcaga agtcaacgcc gacggcacca tcaccgcacc gacttacacc
     1021 attgcgaacg ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc
     1081 ctgctgtggg atgagactgc caatggcggt gctggagcct acaatgccag ccatgacggt
     1141 aaagccagca tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca
     1201 gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc
     1261 aatcagctcg ctggtaacac cgacgcaacc tatatccaag aaaacggtgc gggtattaac
     1321 tatgtgcgta ctaacgacga cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc
     1381 gctacagcta taggttataa ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag
     1441 ggcagctaca gcgacgttga tacgggtatc gccctgggta gcagctctgt ttccagccga
     1501 gtgattgcca aaggctcccg tgacaccagc ataacggaaa atggcgttgt tattggttac
     1561 aacaccacgg atggcgaact gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt
     1621 caaatcatca acgtagccga tgattccgaa gcccatgacg ccgttacggt tcgtcaattg
     1681 cagaatgcga ttggtgctgt agcaacgaca ccgtccaaat acttccatgc taattcaacg
     1741 gaagaagatt cactggcagt gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg
     1801 aatggcgata aaggtattgg tatcggttat ggtgcctacg tggacgcgaa tgcacttaac
     1861 ggcattgcca ttggtagcaa tgcgcaagtc attcatgtca acagtattgc gataggtaat
     1921 ggttctacga ccactcgtgg cgctcaaacc aattataccg cctacaacat ggacgcaccg
     1981 cagaactctg tcggtgaatt ctcagttggt agtgcggatg gtcaacgtca gatcactaac
     2041 gtcgcagcag gttcggctga taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat
     2101 gcgcaggttt cccagaatac ccagagcatt actaacctgg ataatcgggt aacgaatctt
     2161 gattcacgcg tcaccaatat cgaaaacggt attggcgata tcgtcaccac cggtagcacc
     2221 aagtacttca agaccaatac cgatggtgta gatgccagcg cgcagggtaa agatagcgtt
     2281 gctattggtt ctggttccat tgctgccgct gacaacagcg tcgcattggg taccggttcc
     2341 gttgcagagg aagaaaatac aatctctgta ggttcttcta ctaaccaacg ccgtatcacc
     2401 aacgtagctg ccggtgttaa tgccaccgat gcggttaacg tgtcgcagct gaaatcttct
     2461 gaagcaggcg gcgtgcgcta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc
     2521 accctcggtg gcggcaacgg cggtacgact cgtatcagca acgtctccgc tggcgtcaac
     2581 aacaacgacg cggtgaatta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac
     2641 accgatcagc gaatggttga gatggataac aaactgtcta aaactgaaag caagttgagc
     2701 ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttacac tccaggtgcc
     2761 agcatggcct ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta
     2821 tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag
     2881 ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg
     2941 tgactaaggg caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc
     3001 tccgggtcaa caatctaagg gcaattctct gatgaggatt gcccttttct ttaccagaca
     3061 tctcccccca caagaattgg ccctaccaat tcttcgctta tctgacctct ggttcacaat
     3121 tccccaatta aaactcacat caatattgcc aatacataac atttagttaa ccattcattg
     3181 tcattatccc tacacaacac aattggcagt gccactttta cacaacgtgt gacaaggaga
     3241 tgagcaacag actcattaca cgatgtgcgt ggactccagg agacctgcaa tgaatctctg
     3301 gcaacaaaac tacgatcccg ccgggaatat ctggctttcc agtctgatag catcgcttcc
     3361 catcctgttt ttcttctttg cgctgattaa gctcaaactg aaaggatacg tcgccgcctc
     3421 atggacggta gcaatcgccc ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa
     3481 cgcgctggcc tcggtggttt atggcttctt ctatggtttg tggcccatcg cgtggatcat
     3541 tattgcagca gtgttcgtct ataagatctc ggtgaaaacc gggcagtttg acatcattcg
     3601 ctcgtctatt ctttcgataa cccctgacca gcgtctgcaa atgctgatcg tcggtttctg
     3661 tttcggcgct ttccttgaag gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc
     3721 attgctggtc ggcctgggtt ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa
     3781 caccgcgcca gtggcatttg gcgcgatggg cattccaatc ctggttgccg gacaagtaac
     3841 aggcatcgac agctttgaga ttggccagat ggtggggcgg cagctaccgt ttatgaccat
     3901 tatcgtgctg ttctggatca tggcgattat ggacggctgg cgcggtatca aggagacgtg
     3961 gcctgcggtc gtggttgcgg gcggctcgtt tgccatcgct cagtacctta gctctaactt
     4021 cattgggccg gagctgccgg acattatctc ttcgctggta tcactgctct gcctgacgct
     4081 gttcctcaaa cgctggcagc cagtgcgcgt attccgtttt ggtgatttgg gggcgtcaca
     4141 ggttgatatg acgctggccc acaccggtta cactgcgggt caggtgttac gtgcctggac
     4201 accgttcctg ttcctgacag ctaccgtaac actgtggagt atcccgccgt ttaaagccct
     4261 gttcgcatcg ggtggtgcgc tgtatgagtg ggtgatcaac attccggtgc cgtacctcga
     4321 taaactggtt gcccgtatgc caccagtggt cagcgaggct acagcctatg ccgccgtgtt
     4381 taagtttgac tggttctctg ccaccggcac cgccattctg tttgctgcac tgctctcgat
     4441 tgtctggctg aagatgaaac cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga
     4501 actggctcta cccatctact ccatcggcat ggtgctggcg ttcgccttta tctcgaacta
     4561 ttccggactg tcatcaacac tggcactggc actggcgcac accggtcatg cattcacctt
     4621 cttctcgccg ttcctcggct ggctgggggt attcctgacc gggtcggata cctcatctaa
     4681 cgccctgttc gccgcgctgc aagccaccgc agcacaacaa attggcgtct ctgatctgtt
     4741 gctggttgcc gccaatacca ccggtggcgt caccggtaag atgatctccc cgcaatctat
     4801 cgctatcgcc tgtgcggcgg taggcctggt gggcaaagag tctgatttgt tccgctttac
     4861 tgtcaaacac agcctgatct tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta
     4921 tgtcttaacg tggatgattc cttaatgatt gttttaccca gacgcctgtc agacgaggtt
     4981 gccgatcgtg tgcgggcgct gattgatgaa aaaaacctgg aagcgggcat gaagttgccc
     5041 gctgagcgcc aactggcgat gcaactcggc gtatcacgta attcactgcg cgaggcgctg
     5101 gcaaaactgg tgagcgaagg cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc
     5161 tggcgtcatg acacatggtc ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc
     5221 gatgatccgg attacagttt cgatattctg gaagcccgct acgccattga agccagcacc
     5281 gcatggcatg cggcaatgcg cgccacacct agcgacaaag aaaagattca gctttgcttt
     5341 gaagcaacgc taagtgaaga cccggatatc gcctcacaag cggacgttcg ttttcatctg
     5401 gcgattgccg aagcctcaca taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat
     5461 gtcctgcaat cctcagtgaa gcatagccgt cagcggatgt atctggtgcc accggttttt
     5521 tcacaactga ccgaacaaca tcaggctgtc attgacgcca tttttgctgg tgatgccgac
     5581 ggggcgcgta aagcaatgat ggcgcacctt agttttgttc acaccaccat gaaacgattc
     5641 gatgaagatc aggctcgcca cgcacggatt acccgcctgc ccggtgagca taatgagcat
     5701 tcgagggaga aaaacgcatg attatttccg cagccagcga ttatcgcgcc gcagcgcaac
     5761 gcattctgcc gccgttcctg ttccactata tggatggggg tgcatattct gaatacacgc
     5821 tgcgccgcaa cgtggaagat ttgtcagaag tggcgctgcg ccagcgtatt ctgaaaaaca
     5881 tgtccgactt aagcctggaa acgacgctgt ttaatgagaa attgtcgatg ccggtggcgc
     5941 taggtccggt aggtttgtgt ggcatgtatg cgcgacgcgg cgaagttcag gcagccaaag
     6001 cggcggatgc gcatggcatt ccgtttactc tctcgacggt ttccgtttgc ccgattgaag
     6061 aagtcgcgcc agccatcaag cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg
     6121 gctttatgcg taacgcgctg gagcgagcaa aagcagcggg ttgttcgacg ctggttttca
     6181 ccgtggatat gccgacaccg ggagcgcgtt atcgtgatgc gcattctgga atgagcggcc
     6241 cgaacgcggc aatgcgccgc tatttgcagg cggtgacgca tccgcaatgg gcgtgggatg
     6301 tgggcctgaa cggtcgtccg catgatttag gaaatatttc ggcttacctc ggcaaaccga
     6361 ccggactgga agattacatc ggctggctgg ggaataactt cgatccgtcc atctcatgga
     6421 aagaccttga gtggatccgc gatttctggg atggcccgat ggtgatcaaa gggatcctcg
     6481 atccggaaga tgcgcgcgat gcagtacgtt ttggtgctga tgggattgtg gtttctaacc
     6541 acggtggccg ccagctggac ggtgtgctct cttctgcccg tgcactgcct gctattgccg
     6601 atgcggtgaa aggtgatatc gccattctgg cggatagcgg aattcgtaac gggcttgatg
     6661 tcgtgcgtat gattgcgctc ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg
     6721 cgctggcaac agcgggccag gcgggtgtag ctaacctgct aaatctgatc gagaaagaga
     6781 tgaaagtggc aatgacgctg actggcgcga aatcgatcag tgaaattacg caagattcgc
     6841 tggtgcaggg gctgggtaaa gagttgccta cggcactggc tccgatggcg aaagggaatg
     6901 cggcatagtc gtttgagccc cctcacccta accctctccc tcagggagtg gggaccgttc
     6961 ggcgctgtat gtactccctc actctgaaac gacaccgcac tctttttttc tccctcgccc
     7021 ctccggggtg aggggaaaag gccgcactgc tcaccaattt tctgctatcc tgcccccgca
     7081 ctaagggggc agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg
     7141 caacatcatc cgtctttgcg ctaataccgg ctttcgtctg catatcatcg aaccgatggg
     7201 atttgcctgg gacgataagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc
     7261 cgttacgcgt catcatgact atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt
     7321 tgccctcacc acgaaaggta cgcctgctca cagcgccgta agctatcagg atggcgatta
     7381 tctgatgttc ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgc
     7441 tgaacaaaaa attcgcattc cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc
     7501 ggtgtcggta gtggtgtatg aagcctggcg gcagctggga tatccgggag cgttgttgag
     7561 agattagtta ctgtatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat
     7621 tagatcccat ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc
     7681 attgatggct tatcgctgtc tggtttaccg acaatacgag ccggaacgcc agcggcggtt
     7741 gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcgacca
     7801 acttcaatat tgccgaggat tttcgcgccc gcgccaatca tcacaccttc acgaattttc
     7861 gggtgacggt caccaccaga tttacccgta ccgccaagcg tcacagattg cagaatcgat
     7921 acgtcgtttt caattaccgc cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata
     7981 atgccacggc caatttttgc tgccgggtga atatcgacct ggaacgtcac agaaacctgg
     8041 ttttgcagaa agattgccaa tgcgcgacgc ccctgattcc acaaccagtg accgatgcga
     8101 taggcctgca aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact
     8161 gccgggtcgc gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg
     8221 tcagcggcgt aggcttcttc caccacttca cggatagcaa tagcaggcat aattggcgat
     8281 gacagcttgt tcgccagcat gtagctcagt gcactaccaa ggttttcgtg cttgagtagc
     8341 gtcgcgtggt aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct
     8401 ttaatattgt tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga
     8461 gataatgacc ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg
     8521 ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat aacgtcaatg ctgcctcgcg
     8581 cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc
     8641 gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc
     8701 aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatgc caaaacggcg
     8761 gttacgcgac tggttgtcgg tacaggtaag caccagatcg ccaagccccg ccatgcccat
     8821 aaaggtggca ggatcggcac ccagcgccgc accaagacgc gacatttcag ccagcccacg
     8881 ggtgatcagc gccgtacgcg cattcgcacc aaaaccgata ccgtcggaca tccccgcgcc
     8941 aatggcaatg acgtttttca ccgcaccgcc aagctgcaca ccgatgaaat ccgggttgct
     9001 gtaaacgcgg aaacttttgc cacagtgcag caattgttgg agatcatcgg caaaggtctg
     9061 gtcggtagag gccaacgaaa tagccgtcgg taaacctgcc gccagttctt tcgcaaacgt
     9121 tgggccagag ataaccgcca gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg
     9181 taacagacgt ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg
     9241 acgcatcagc ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac
     9301 gagaatatta cggctggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc
     9361 gggaaaaggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat
     9421 atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtaatggc
     9481 aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat
     9541 caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac
     9601 agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg
     9661 ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa
     9721 tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca
     9781 cacaggaacg cggtttcttc gcccagggag gccgttacgg taacacgcag taccacttcg
     9841 taaacgtcat ctgccagttg ggaagatgcc gtatccagat caagtttaac ttctggctgc
     9901 caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagaaatatc cttggtataa
     9961 atacgttgga tctggaaagt catttcggtg ttgttttgtt ctgacatgtg tagaaaaccc
    10021 ttaagtgttg tccttaaata ctgcgcaatg taatgccaac gttcacacgt tatttcagca
    10081 ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct
    10141 gtgcgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac
    10201 gcttggcggc attgccatcg atcggcaact cctgaaaact cacgcccttg ctgctcagca
    10261 gtgcttttgc acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca
    10321 taatttagct cctgtttttt taccctgaaa tttcatgttg cagggcggct gcaaaatgga
    10381 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    10441 atcatgaaag acgacaggta aattatttgc cgcgcaccaa aggcaagttt tcgccagccc
    10501 agccagcgac gccttctttc agcacgaata cttgcgcaaa accagctttc gtcagtgcgt
    10561 ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccggt ttgtctttgt
    10621 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac
    10681 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    10741 ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc
    10801 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    10861 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    10921 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    10981 actggaatgc aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc
    11041 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    11101 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    11161 tggtactggt gattctggat ggctatggct atcgtgaaga acagcaggat aacgccattt
    11221 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    11281 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    11341 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    11401 tcaaagatcg cgctttcttt gctaatccag tgctgactgg cgcggtagat aaagcgaaaa
    11461 acgcaggtaa agcagtacac attatgggtc tgctctccgc aggcggcgta cacagccacg
    11521 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    11581 tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa
    11641 aatttgaaga gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    11701 actacgcgat ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga
    11761 ctctggcgca gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg
    11821 ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    11881 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    11941 gcgaaatcac tcgtgcattc gtaaacgcag atttcgacgg cttcgcgcgt aagaaagtgg
    12001 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    12061 acccacccgc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    12121 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    12181 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    12241 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggccatca
    12301 agagcggcaa atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca
    12361 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    12421 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    12481 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    12541 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg
    12601 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    12661 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    12721 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    12781 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    12841 ctattcaggc cgatatcgcc gcgaaagagc gcgcggtacg ccaaaagcaa caacaacgcg
    12901 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    12961 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    13021 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg
    13081 atgccgcatt ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa
    13141 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    13201 ccattgccca gttgaagcaa acgcgtgaag aagtcgccat gcagcgtgct gaactggaag
    13261 agaaacagag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga
    13321 ctcaggctct gaacgagcgt aaaaagacgc tggcggggct ggaatcttcc atccagcaag
    13381 gtcagcagca gctaagtgaa ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc
    13441 gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc
    13501 gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta taaaccgacc gaaagcgaaa
    13561 aatcgctgat gtcccgaact ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg
    13621 ttcgcgggcc gacgctgcat cgctatggtg aacagctaca gggcgaacta cgctggaaag
    13681 gaatggttat cggtgcctct gaaggtactg aagttaaagc gattgccgac ggtcgggtga
    13741 ttctggctga ctggctgcaa ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg
    13801 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    13861 cgggccagcc aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    13921 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    13981 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    14041 tacttgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    14101 accaggtgct ggcgatgccc tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    14161 ccagagagat ggcgaccaaa gcgcataaca gcgggcacga agtgttgatt catctcccga
    14221 tggcaccact gagtaaacag ccgctggaga aaaatacgct gcgcccggag atgagcagcg
    14281 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    14341 acaaccacat gggtagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    14401 cactggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaggcga
    14461 tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt
    14521 cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca
    14581 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    14641 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    14701 cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt
    14761 tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    14821 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    14881 agtggcaagg ctggggcaaa cagcccgaag cggcgaagtt taacgctagc gcaaattaaa
    14941 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    15001 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    15061 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    15121 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    15181 ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    15241 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    15301 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcgtt
    15361 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    15421 gttattttta acaataacat cacggcgata aacccccatc cagacaacgt gagtccaacg
    15481 gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacgccgg ttgagcgaag
    15541 gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt
    15601 gcactgcgcc acgtcgaggt catcctctaa cgccatggtc atcagcgttt cgtacatggt
    15661 gggatagacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg ccacctcgat
    15721 cccacgatta cgcgctaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    15781 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    15841 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    15901 atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc tgttcatcat
    15961 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    16021 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    16081 gagagcagct atataagaga tcagcgcaat tacgatatct ttattacact gacaggattt
    16141 tacatattta acggattatc ctcaggcaaa agggctggaa taccagccct tgttcgtgtt
    16201 aatcccaact cagaattact ttcccggact ggcccgaacg catagcgtca aagcccttct
    16261 ggaaatcatc gatagagaaa cgatgggtaa tgatcggcga aagatcgagg ccagactgaa
    16321 tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaata cctttaatga
    16381 acaagccttt aaagatcact ttggtccagt cgatagacat atcagacggc ggaataccca
    16441 gcatcgcaat acggccgccg tggttcatgg tatcaagcat ggtacgaaac gctggcggcg
    16501 caccggacat ttccagaccg acatcaaaac cttcggtcat gcctaactcc gccatcacgt
    16561 cattgagatt ttctttggcg acgttaaccg cacgggtgat acccatttta cgcgccagct
    16621 caaggcggta ttcgttaaca tcagtgatca ccacattgcg tgcaccaacg tgtttcgcca
    16681 ccgccgctgc cataatacca atcgggcctg caccagaaac cagcacatcc tcgccaacca
    16741 gatcgaacga cagcgcggta tgcacggcat taccgaaggg atcaaaaatg gaagccaggt
    16801 cgtcggagat attgtcggga attttgaagg cgttgaacgc cggaatcacc agatattcgg
    16861 cgaagcagcc cgggcggtta acgccgacgc cgatcgtgtt gcggcacaga tgggtacgac
    16921 cgccacgaca gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc
    16981 caatcttgaa gcccttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    17041 ccacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    17101 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    17161 ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt
    17221 tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    17281 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    17341 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatgccctct ttttgcagct
    17401 cacgggcaaa tttctgcgcc actaccgcat caccaagcat gaccggaata atggcgtgat
    17461 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga cgcgcgttcg
    17521 cccacagacg gtcacgcaac tcgctacccg cttcaaccat ctccagcact ttgatggaag
    17581 cggcgacaat cgctggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca
    17641 gccactcaac cacttctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt
    17701 taccgagcgt gccggtgatg atgtcgacac gccccatcac atcgcagtat tcatgggaac
    17761 cacgaccatt ttcaccgaca aaaccgaccg cgtgggagtc gtctaccatc accagggcat
    17821 cgtatttatc tgccagatcg caaacgccct tcagattggc aatcacgccg tccatcgaga
    17881 acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    17941 gagcttcaag ctcctgcata tcgttgttgg catagcgata gcgtttagct ttacacagac
    18001 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    18061 ccagcagcgt ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    18121 cttccatccc caggaatgcc gccagttttt gttcaagctc tttatggctg tcctgagtgc
    18181 cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgctg
    18241 ccgcaatcag atccggatga ttcgccagcc cgagatagtt gttggcacaa aagttaatga
    18301 cgtggcttcc atcagccacg gtgatatctg cttgctgcgc agacgtaata atgcgctctt
    18361 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    18421 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    18481 ggtcgcgacg agataacgcg ttaacacttc tgcaaaattc aggataaata acgtccatca
    18541 atctcacttt tggcgaatgg ctgaagccaa cgccgtgtag tgatatgata gaagtattcg
    18601 tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg
    18661 cggcgcgggc tttatcggca gcaacatcgt taaggccctg aatgataaag gcatcaccga
    18721 tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgga
    18781 tatcgcggac tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt
    18841 cggcgatgtc gaagcgattt tccacgaagg cgcatgctct tccaccaccg agtgggacgg
    18901 caagtatatg atggataaca actatcaata ctccaaggag ctgctgcact actgcctgga
    18961 gcgcgaaatc ccgttcctgt acgcttcttc cgcagccacc tacggcggac gcacctccga
    19021 ctttattgaa tcccgcgagt acgaaaaacc gttgaacgtc tacggttact caaaattcct
    19081 gtttgatgaa tatgttcgcc aaatcctgcc tgaagcgaac tcgcagattg ttggcttccg
    19141 ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt
    19201 ccatctcaac actcagctta acaaaggtga atcaccgaag ctgtttgaag gtagcgagaa
    19261 cttcaaacgt gacttcgtct atgtgggcga cgtggcagat gtaaacctgt ggttcctgga
    19321 aaatggcgtt tccggtatct tcaacctcgg tactggtcgt gcggaatcct tccaggccgt
    19381 agcagatgct actctggctt atcacaagaa aggccagatc gaatacattc cgttcccgga
    19441 taagctgaaa ggccgctacc aggcgttcac tcaggcagat ctgacaaatc tgcgcgcggc
    19501 gggttacgac aaaccgttca aaaccgttgc tgaaggtgta atggaataca tggcctggct
    19561 gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg
    19621 cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat
    19681 aatcgatgtg atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa
    19741 cgaagctatc cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact
    19801 gggtcatagc ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccaa actccttcaa
    19861 atctgcatta gtgcctttct tcgcgggtat tcctcatcgc actggctggc gcggcgagat
    19921 gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt
    19981 ggaacgctat gtcgcgctgg cctatgacaa aggcattatg cgtaccgcac aagatctgcc
    20041 gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa
    20101 tcaattttcg ctttcatcag aacgtccgat gattggcttt tgcccgggtg cggagtttgg
    20161 tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga
    20221 aggttatcag gtggttctgt ttggctctgc gaaagatcat gaagcgggca atgagattct
    20281 tgccgctttg aataccgagc agcaggcatg gtgtcggaac ctggcggggg aaacacagct
    20341 tgatcaagcg gttatcctga ttgcagcctg taaagccatt gtcactaacg attctggcct
    20401 aatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtttggtc cgagtagccc
    20461 ggacttcaca ccgccgctat cccataaagc gcgcgtgatc cgtctgatta ccggctatca
    20521 caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg acattactcc
    20581 ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg
    20641 ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgttgc ccgcactcac
    20701 tgatgcccag caggcaatcc cagggattaa gtttgactgg gtggtggaag aagggttcgc
    20761 acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg
    20821 gcgtaaagcc tggttctcgg cccccataaa agctgaacgc aaagcgtttc gtgaagcgct
    20881 acaagcagag aactatgacg cagttatcga tgctcagggg ctggtaaaaa gcgcggcgct
    20941 ggtgacgcgt ctggcgcatg gcgtaaagca tggattggac tggcaaaccg ctcgcgaacc
    21001 gttagccagc ctgttttaca atcgtaagca tcatattgca aaacagcagc acgccgtaga
    21061 acgcacccgc gaactgtttg ccaaaagtct gggctatagc aaaccgcaaa cccagggcga
    21121 ttatgctatt gcacagcatt tcctgacgaa cctgcctaca gatgctggcg aatatgccgt
    21181 atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt
    21241 gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg cgccgcatga
    21301 ggaagaacgg gcgaaacgac tggcagaagg atttgcttat gttgaagtat tgccgaagat
    21361 gagtctggaa ggcgttgccc gcgtgctggc cggggctaaa tttgtagtgt cagtggatac
    21421 ggggttaagc catttaacag cggcactgga tagacccaat atcacggttt atggacctac
    21481 cgatccggga ttaattggtg ggtatgggaa gaatcagatg gtttgtaggg ctccggggaa
    21541 tgagttgtct caattgacag caaatgctgt taagcggttc attgaagaaa acgctgccat
    21601 gatttaaatt acaact
//
LOCUS       DAPHJE010000076        21363 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPHJE010000076 DAPHJE010000000
VERSION     DAPHJE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.076"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..480
                     /gene="nudI"
                     /locus_tag="RH222_002912"
     CDS             55..480
                     /gene="nudI"
                     /locus_tag="RH222_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV3797901.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(519..1121)
                     /locus_tag="RH222_002913"
     CDS             complement(519..1121)
                     /locus_tag="RH222_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV3797902.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            1429..2568
                     /gene="arnB"
                     /locus_tag="RH222_002914"
     CDS             1429..2568
                     /gene="arnB"
                     /locus_tag="RH222_002914"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV3797903.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPDWPHVHAWHLFIIRVDEQCCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            2572..3540
                     /gene="arnC"
                     /locus_tag="RH222_002915"
     CDS             2572..3540
                     /gene="arnC"
                     /locus_tag="RH222_002915"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV3797904.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            3540..5522
                     /gene="arnA"
                     /locus_tag="RH222_002916"
     CDS             3540..5522
                     /gene="arnA"
                     /locus_tag="RH222_002916"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV3797905.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFTYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            5519..6409
                     /locus_tag="RH222_002917"
     CDS             5519..6409
                     /locus_tag="RH222_002917"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV3797906.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            6409..8061
                     /gene="arnT"
                     /locus_tag="RH222_002918"
     CDS             6409..8061
                     /gene="arnT"
                     /locus_tag="RH222_002918"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV3797907.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFEIYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            8058..8393
                     /gene="arnE"
                     /locus_tag="RH222_002919"
     CDS             8058..8393
                     /gene="arnE"
                     /locus_tag="RH222_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV3797908.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            8393..8779
                     /gene="arnF"
                     /locus_tag="RH222_002920"
     CDS             8393..8779
                     /gene="arnF"
                     /locus_tag="RH222_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV3797909.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(8773..9039)
                     /gene="pmrD"
                     /locus_tag="RH222_002921"
     CDS             complement(8773..9039)
                     /gene="pmrD"
                     /locus_tag="RH222_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV3797910.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(9149..10504)
                     /gene="menE"
                     /locus_tag="RH222_002922"
     CDS             complement(9149..10504)
                     /gene="menE"
                     /locus_tag="RH222_002922"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV3797911.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPIELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(10501..11463)
                     /gene="menC"
                     /locus_tag="RH222_002923"
     CDS             complement(10501..11463)
                     /gene="menC"
                     /locus_tag="RH222_002923"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV3797912.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(11463..12320)
                     /gene="menB"
                     /locus_tag="RH222_002924"
     CDS             complement(11463..12320)
                     /gene="menB"
                     /locus_tag="RH222_002924"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV3797913.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(12335..13093)
                     /gene="menH"
                     /locus_tag="RH222_002925"
     CDS             complement(12335..13093)
                     /gene="menH"
                     /locus_tag="RH222_002925"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV3797914.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(13090..14760)
                     /gene="menD"
                     /locus_tag="RH222_002926"
     CDS             complement(13090..14760)
                     /gene="menD"
                     /locus_tag="RH222_002926"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV3797915.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(14849..16144)
                     /gene="menF"
                     /locus_tag="RH222_002927"
     CDS             complement(14849..16144)
                     /gene="menF"
                     /locus_tag="RH222_002927"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV3797916.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNRRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVHGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(16223..16528)
                     /gene="elaB"
                     /locus_tag="RH222_002928"
     CDS             complement(16223..16528)
                     /gene="elaB"
                     /locus_tag="RH222_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV3797917.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDTADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(16583..17044)
                     /locus_tag="RH222_002929"
     CDS             complement(16583..17044)
                     /locus_tag="RH222_002929"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3797918.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            17109..18026
                     /gene="rbn"
                     /locus_tag="RH222_002930"
     CDS             17109..18026
                     /gene="rbn"
                     /locus_tag="RH222_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV3797919.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            18214..19434
                     /gene="elaD"
                     /locus_tag="RH222_002931"
     CDS             18214..19434
                     /gene="elaD"
                     /locus_tag="RH222_002931"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDV3797920.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            complement(19505..21259)
                     /locus_tag="RH222_002932"
     CDS             complement(19505..21259)
                     /locus_tag="RH222_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV3797921.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESQQQQLSTPTDQQVLAA
                     QQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKA
                     ASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAV
                     RVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKS
                     EELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALP
                     SISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNV
                     GIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQ
                     KVLNSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIG
                     AGKHITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLV
                     EFPLGPTINAPSEDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYR
                     AEFIRLIELADGVTDISQ"
BASE COUNT         5220 a         5567 c         5205 g         5371 t
ORIGIN      
        1 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctatgcga
       61 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgtaaaatg
      121 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
      181 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
      241 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacgtatgca
      301 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
      361 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
      421 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
      481 caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac gaattcccca
      541 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
      601 aatgtcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
      661 acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
      721 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
      781 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
      841 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
      901 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
      961 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
     1021 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
     1081 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctatagac
     1141 aagcgcaacc agacaattac cgtaaaattg agcgacattt ctggcgataa ttagcagttg
     1201 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaaattttgc caaatgcaaa
     1261 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
     1321 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
     1381 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
     1441 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
     1501 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
     1561 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
     1621 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
     1681 tcaaccctca atatgatttc cttgttgggt gcaacgccgg taatggtgga tgtcgaccgc
     1741 gatacgctga tggtcacgcc tgaagcgatc gagtcagcca ttacgccacg cactaaagcc
     1801 atcattccgg tgcattatgc cggtgcgcca gcagatattg acgccattcg cgccattggc
     1861 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
     1921 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
     1981 acctgtgcgg aaggtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
     2041 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
     2101 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
     2161 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
     2221 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgactgg
     2281 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaatg ttgtggtatc
     2341 agtcgaaatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
     2401 cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
     2461 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
     2521 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
     2581 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
     2641 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
     2701 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
     2761 gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca acattcagcg
     2821 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
     2881 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
     2941 gttgggactg tacgccagaa ccgccaggac agctggtttc gtaaaaccgc ttcgaagatg
     3001 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
     3061 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
     3121 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
     3181 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
     3241 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg tagcattatt
     3301 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
     3361 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
     3421 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
     3481 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
     3541 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
     3601 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
     3661 tttatggttc ggtggctcat ctggcggcgg aaagaggcat tccggtttat gcgccggata
     3721 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
     3781 ctttttatta tcgccatctt atttgcgacg agattttgca gctcgctccc gcaggtgcat
     3841 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
     3901 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
     3961 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
     4021 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
     4081 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
     4141 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
     4201 tacgtgccgt tgccgatccg tggccgggtg ccttcaccta tgttggcaat cagaaattta
     4261 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
     4321 ctattgcacc gctgttgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
     4381 cgggcgacgg cattactatg cagggctcgc aattagcgca gacgctgggc ctggtgcaag
     4441 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
     4501 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
     4561 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccgc
     4621 attttcactt tgttgaaggc gatatcagta ttcattccga atggattgag tatcatgtca
     4681 aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
     4741 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
     4801 tgaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
     4861 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
     4921 ggatttattc ggtgtcaaaa caattacttg atcgggttat ctgggcctat ggcgaaaaag
     4981 agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga ctggataacc
     5041 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
     5101 aaggttcacc gattaagctg attgatggcg gaaaacaaaa acgctgcttt actgatattc
     5161 gcgatggtat cgaggcgtta taccgcatta tcgaaaatgc gggaaatcgc tgcgacggtg
     5221 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
     5281 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
     5341 gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag catcgtaaac
     5401 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
     5461 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
     5521 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
     5581 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
     5641 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
     5701 gctgcgctca aatgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
     5761 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
     5821 cgaagtcggc ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
     5881 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
     5941 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
     6001 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc cgttccgtcc
     6061 attgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
     6121 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
     6181 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
     6241 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
     6301 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
     6361 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
     6421 cgttacctta tcggcctctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
     6481 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
     6541 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
     6601 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
     6661 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
     6721 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
     6781 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
     6841 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
     6901 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
     6961 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
     7021 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ttggggactg
     7081 gcgatagcgc agcgagagcc taacttctgg cactattttt tctgggttga gcatattcaa
     7141 cgctttgcac tggatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
     7201 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
     7261 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
     7321 ttctccgtcg ctaaaggtaa attgcccacc tatattcttt cctgctttgc atctctggca
     7381 atgctgatgg cgcattacgc tttgctggca gcaaaaaata atcctctggc gctgcggatt
     7441 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtggtctcc
     7501 ccctggggac caatgaacac gccggtgtgg caaaccttcg agatctataa agtcttttgt
     7561 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
     7621 aagacctggt cttttgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
     7681 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
     7741 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggcg
     7801 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
     7861 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
     7921 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
     7981 atcaacagtc tcgccattcc gcccgcagat gccatcgatc gtcaggagcg tctggtgctg
     8041 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
     8101 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
     8161 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc
     8221 tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
     8281 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcaccg cgtcactggt
     8341 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
     8401 gatgtgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
     8461 ggcgagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
     8521 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
     8581 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
     8641 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
     8701 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccat
     8761 gacaaaacaa cgttactgag ttttccctgc cactttacac tgccgttccc actcatcggg
     8821 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
     8881 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
     8941 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
     9001 ttgtttattg cagcacgatt ttttgaccag ccattccatt gcattatcct gtttgctaag
     9061 agttttcaca tcaatttaca gattaacaga taaactcttt atcaacggca atggaatggc
     9121 agaaatgaga agtgaaacgg agggttaatt attgctgacg ttgcacccac tcttttagcg
     9181 cctgacgtga aattttaata ccgccgtttt tcagttcagg cggcaacgtt aaccaacgca
     9241 ccggttgctg aaaactggcc agcttatctt tcacccattc actaagatca acgctctcgt
     9301 ggtcatactc catcaccgcc accggtcgat gaccaaactc cttgtcggca acggggacga
     9361 taaacacctg caaaaccgca ggatgtgcag caataacacg ctcgacttct tccggctgaa
     9421 taccctctcc gccactgaag aatagattgt ctaaacgtcc gacaatggtc agcttgccat
     9481 tatgcatctc accgcgatcg cgcgtagcgt accagccttc gtcattaacc agtgaaacca
     9541 gttgcccgtt acgccagtaa ccttctgcca tactggcagc ccgcagccac acttcattat
     9601 taacgatttt cacttcccga cccggcagcg gcgaaccaac gtctgccagg ccgtcggctt
     9661 ctttcgcaca caccgtggag gcaaactcgg tcagaccata gccgcaaaag caacgaatcc
     9721 cctgctcgcg cgcctgttcc gtcaactcga tcgggatagc cgcgccgcca agtaacaccg
     9781 ctttcaggga aacggaactg cggttaacca gcaaacgcca gagttgtgtt ggcaccagtg
     9841 aagcatgagt acagcctgcc agcatttgct ccaatggctg tttatcacgc accgtcatcc
     9901 gcgcaccagc gtataaccag cgccacataa ttccctgacc ggagacgtga aacagcggta
     9961 aagagagcaa ccagtcatcg tgatcgccaa acggaatcaa cgataacacc ccttcggcac
    10021 tggcaagatg ggcctgataa gtatggacag cggctttcgg caacccggta gaacccgagg
    10081 tcaacgtcat tgagcacaga cgcgtcggct gccacgtagc ggcatgtgcg ccttcaaccc
    10141 gctgaatgtg cagcgacgtt aacgccggaa acgtgttttc cccatccggc accagcgcaa
    10201 attgcagcgt cagattgggc agcaattctt caagcaacgg ttgcggcagc tgagggttca
    10261 cgggcaacac ccgcgccccg cattgcagta acgccagcca ggcgagcagc gtttgcggcg
    10321 tattccacgc ccgcaacatc acgccgctgc cctcaacaac gccctgcgcc gcaaagcccg
    10381 tagctaactc atcgacgcga gcacaaagct cgcgccagtt aagttgctcg tcattaagac
    10441 gtaaggcgat ggcttctccc cgcacttgcc gccagtgacg ccacggccag tcagagaaga
    10501 tcataacaac cgctccagtg catcaacttc cacgacaggc agcgtgctac ccggccagcg
    10561 acgtacctgc tgcgcctgca tcagatccag cgtgtccagc cctggaatgg tgtccggcgt
    10621 taaccaggcg gcaatccgcg ccagttgcgt taagcctaag ctcgattcaa tggaagaact
    10681 gatcaccgcc gtcagcccca gcgcgtgcgc cgcctgtacc tgctcgcgta ctttctccag
    10741 actgcccgtg agcgtgggtt tgataactac cgcgcgcacg ccctcttcag ccacaaaggc
    10801 aaaatccggc tcgcgcaagc tttcatccca ggcaatggca atgccggttt cacgggcaaa
    10861 cgctcgcgaa tcatcgcggg ttttgcacgg ctcttcgaga aacgcgatgc ggtggcgata
    10921 atccgggtta acatatttgg caaactgctg gcctttcagc ggtgtccagg cgcgatttgc
    10981 gtcaagacgc aaatgcagat ccggaattgc ctccagcaac agattaacca ccatgccgtc
    11041 gcgcacggct tcgtacaatc ccactttgac cttcgccact ttctcgcctg gcatatcagc
    11101 aagtttgagg atcagatcgt caggatcgcc attacacagt ggtgccgcac ggtaattggc
    11161 cgcctgcggg agcgtatctg ccagctccgc caatgcacag cttacgccaa aggcaacgga
    11221 agtcatctgc ggtagctcgc aatcgcctgc cagccagtta tttacccagg caagcagcac
    11281 gctttgcgcc tcttcccagg tttcctggct gaagcccggc agtggggaga tctcccccca
    11341 cccttcgcgc tcgccctcac gcaggcaaac gtacagcccg tcgcgggttt ttaaccgcct
    11401 gtcgcgcaga accacccccg cgtccatggg gatctgccag cggtatacct gcgcgctacg
    11461 cattacggat tccgtttaaa tttgctgaaa tcaggctgac gtttctggtt gaaagcgttg
    11521 cgaccttcct gaccttcttc cgtcatgtag aacagcatgg tggcgttgcc cgccagctcc
    11581 tgcagccccg cctgcccgtc acagtcggcg ttcagcgcag ctttcaggca gcgcagcgcc
    11641 atcgggctgt tttgcagcat ttcgcggcac caacggacgg tttctttttc cagatccgcc
    11701 agcggtacca cggtgttgac aaggcccata tccagcgcct gttttgcgtc gtactgacgg
    11761 cacaggaacc agatttctcg cgcttttttc tgcccgacga tacgagccat gtaggaagcg
    11821 ccccagccgc cgtcgaagga accgactttc gggccagtct gaccgaagat ggcattatct
    11881 gctgcgatag tcaggtcgca catcatgtgc agaacgtgac caccgccgat ggagtagcca
    11941 gccaccatcg cgacaaccgg tttcggacag gtacggatct gacgctggaa gtccagcacg
    12001 ttcaagtgat gtacgccgga atcatcttta tagccgccgt aatcaccacg cactttctgg
    12061 tcaccaccgg agcagaacgc tttatcgcct gcgccagtca gaatgatcac gccgatattg
    12121 tcgtcataac gcgcatctgc cagtgcctgg atcatctctt ttaccgtcag aggacggaag
    12181 gcattgcgca cctgcggacg attaatggtg atttttgcga taccgtcggt ggatttttca
    12241 taacgaatgt cctcgaaacc ttcggagcag tcgtgccatt caaccggtgc gtaaagcatt
    12301 gcttcatcag gataaatcat attgtgtcct ttagtcagaa acgcaagatc tgcgccagac
    12361 ttgcgattac gccagcggga ttttcccgat gcgcgttatg tccggcgcga ggaatgacat
    12421 ggcagtcggc agccagttcc gccgccaggg cgcggaattt gctgtcacgt tcaccacata
    12481 aataataaaa cgcaaatgtg cgggcgctaa ggttagcacg taaatcaggc tggacggcga
    12541 gagaagtcgc ctccagcatg gcggcaaggg ttgcgccatt attgttgctg cgcagcacca
    12601 ccagctcccg gcgttgatcg tcattgagcg aggcaaaaac aggctgttga taccagtcgg
    12661 caaataccgc cgttaacggt tctgtgcaaa aacgctgcgc ccattggcga tcggaacgct
    12721 gacgttccgc acgttgttca gcattttgca gccccggatg cccgccttcg acaataaccc
    12781 cacaaagccc cgtcagcccc tggcaagccg ccatcatcgc cacccgtccg ccaagcgagt
    12841 accccaccag ccagaagtca aggatgttgt aactaaccaa ggttttacgc agtaagtcgg
    12901 tgacatcatc aaatccatcg acgctaatcg ccgccgaacc accatgacct gggagatcaa
    12961 catacaaccg tgagtagtcg gcaaacgcct cgcccacttc ttgccattcg tggcaatcgc
    13021 cggaaaaacc gtgcaaaaac accagccagg gtaaacctgg ttttccgtgt tttgcctgcg
    13081 cgtgcaggat cataaatggc ttacctgcgc cagaagttgc tggagcgttt gcgcaccatc
    13141 ggtgtcgtta accaccattt caatcaccgt ggtggttggc gtgcgccagg cgtcggcaaa
    13201 tgccgtttca agttcctgcc agttttgcgg acgatgatat ttcagctcga acatcgcggc
    13261 ggcgtgctca aaatggacgt tttgcggcat cagatagaaa cgctcacgct cgctttgcgg
    13321 cgttggcaac agcgagaaaa tttgcccgcc gttgttgttc accacaatta ataccagcgg
    13381 cgcagaaacc tgacgcaata acgccagcgc gttgagatcg taaagtgcgg agagatcgcc
    13441 cacaatcgcc agcgtcggtt tgccgcttgc ccgctgaacg cctgcggcgg tcgaaagcag
    13501 tccgtcgata ccgctggccc ctcggttgct gtacaccggg taacctgccg gaagttgcga
    13561 aagcgcatca atcagacgta ccaccaggct gttaccaaca aacaattgcc cctgttcagg
    13621 cagatagtcg cagatgcgat gcgccagttg cgcttcgcca aacgcatcac ggcgggcaat
    13681 aaccgcctgc attgcctgtt ccgccaggcg cgggatttca acgcaccagg gctggcgttt
    13741 ttctgccgga tgcagctcca gccagtcggc aatattggca attaagcgac gtccgcgatg
    13801 gtgtgccgga tcaagtcgcc cttcaatgtc atcaacaatc cagtactctt ctggttcaca
    13861 gcttgcctgc cattgcagga gccgtttccc cgtcaggctg cttcccagtt gcaccacaat
    13921 ttgcgcctgc tgcagctcgc tggtcgcttt ggcattgcct aaccaaagat cggcacacgg
    13981 cagcggctgc ccggtttgtg acagcacatc gccaatcagc ggccagccaa gagtttgcgc
    14041 ccacagggca acttttttgc cctcttccgc actcatgcgc ccggcaacca ccacgccgcg
    14101 cttttgtcgc cagaagaacc agtcgcgctg tttttcactt tccagacgag gcgcttcacg
    14161 cagccacggt ttgtcgtcct gccaccagtc acccagacgc tgttgccagc taagcccggt
    14221 atcgtccatt tcgccataca gcggttcagc aaacgggcag ttgatatgga ctccccccgc
    14281 atgaagcgta ccgagagcgt ggtcgatggt agaaaccagc caacgtgcgg ggatatcctg
    14341 ggtcgggcgc ggcaatgaaa tactgtgcgt ggggtgagag gcgaacattc ccggctggcg
    14401 aattgcctga ttcgcgccgc agtcaattag ctccggcggg cgatcggcgg ttaagagaat
    14461 cagtttttct ccggttaacc cggcttcaat cagtgccgga tagagatttg ccaccgccgt
    14521 gccggaggtc acaatcaccg ccaccggctg cttgctgact ttcgccagcc ccagcgccag
    14581 atgccccaac ccacgctcat cgaaatgggt gtggtgaatg aatgcggaat tctccgccgc
    14641 cgctaacgtt aacggtgtag aacgcgagcc tggggcgata cagatgtgtc tgacgccgtg
    14701 acgcgttaat gcttccagaa tgaccgccgc ccagcgtcgg ttaaatgcgc ttactgacat
    14761 gagtttgtcc ggtatcaata ttgcggctaa gtataaggag ctacaaaaat agattattga
    14821 tatgaatcgg taatgatgcg actcattact attccatttg taataaagta cgcagccctg
    14881 ccgctttgtt gtcgatttcc tgccactctt gctcggggtc ggaaccatgg acaatgcccg
    14941 cgccagcata taatcgcacg acattgccgc taatttttgc tgagcgcagg gaaacgcaga
    15001 attcgctttg ttgtaatgag agatagcccg cagaaccggc gtaccattct cgggtgaacg
    15061 gttcgtgacg ggcgataaac tgtcgcgcca gatcgcgcgg taagccagca actgctgccg
    15121 ttggctgcaa ctgatgtaaa cagatcacat catccgcttt gttgagtgaa gtccagatac
    15181 agcggcgaag atgctgcact ttacgcagac gcagtacctg cggcggtaaa acatccagcg
    15241 tctgggtatc ggcctgtaat cgttggcaga tatcttccac caccagcatg ttctcgcgcc
    15301 ggtttttatc atccgccatc agccactctc ctaactgctg cgcctgctta tcatcaggat
    15361 tatttgctac tgttcccgcc agcgcttcag tacgcagcgc tttgtcacgc cgccgccata
    15421 accgttccgg tgaagagcca agaaaagcat tttcgccatc aaaggccatg taaaaatggt
    15481 agcaattcag attcagtcga cgactggcag ccatcatcgc cgccgcgttg accggacttg
    15541 cgaaatgcag gtcagttgcc cgagcgagca ccactttgtc gagctcacct tcggcgatgg
    15601 ttttcgttgc cagttcgatt aattgcgtcc agcccgtttt gtccggccag tgttgttctc
    15661 gcgtggtggt taaatgtaac ccaggcaagg gcttgatact caccagtgtg gcgataaatt
    15721 cttttgcctg aatcgcatcg tgctgaaggg agctttcgct gaatagcgtc agccgcagcg
    15781 tggctttacc gccacagcgt cgccattcca ggcggggtaa aagtaaattg ccctgcgacg
    15841 ggtcaaaagc attcagcccc caaatgcgta agtcggcgtg ttccgggtgc tggcgaagaa
    15901 aacgttgtgc ctggtccaac gacgtaaaac gggtaatcgc gcccaggacg gcagcttctt
    15961 catcaccatt acgttgttgc cagtagaatt gcgggtacgt ttgctgactg gccagccagc
    16021 tcaaggcatc aaaagcgtct ttgagaggga aaggaatatc gataacccga atgccgggtg
    16081 tcgccggaat ctcttgcgac aaatggcgca gtagattttc cagcgccgta gtaagtgatt
    16141 gcacgcggac ctctccctgt taaaagcctc acattatacg gggtactaca aaaaaaatgc
    16201 agtaccccgg tgtagggagg ttttaacggc gtgccagcaa cagtcctagt accagcccaa
    16261 cggccgcgcc cacaccaatt ccttgccagg gtttttcgtg gacgtagtca tcagcacgat
    16321 aaactgcctg cttcgcccga tagtaataac tgtctgaagc ctggctaacc cgttttttca
    16381 catcatccag cgctttttct gcacgcgctt tcagctcaac atatttctga tcggcggtat
    16441 cgccagagga gcggagcacc tcttccagtg tttcactaag cagcgtcagg tcgtcatcga
    16501 tacgtgtatc accaaactga ttagacattc tcgttctcca tttgcgtaaa acctgtcact
    16561 aactatagac aagggttgtt gattacgcct gaattacctc gcgtgccatc ccaatgtgtg
    16621 ggataccatc ttcttcatag acctccgtca cagggataaa accgaaactc tggtagaagt
    16681 tttgcagatg cgcctgcgcc ccgaggtaaa caggcttatc aggccagtga tgcgtacatg
    16741 tttcgagtgt tttactcatc agctgctgcc ccactttttc gccacgcaac gcttcactga
    16801 caatcacccg acctataacg accggctcaa gatcatcatc acttttcaga atcctcgcat
    16861 atgccaccag ttcatcattt ttccaaccga gaatatggcg attatccccc gtcaaatcat
    16921 caccatcgat atcctgataa gggcaattct gctcgacaac aaacaccgcg caacgcagtt
    16981 gtaataaggc ataaagctgg gaaaccgata attcagagtg atgcagatct tgccattcaa
    17041 tcataatgct ctccttttag atgtatgcct tatactagcc ggaatatttt ttgaaacgtg
    17101 atgaactcat ggaattaatt tttttaggta cttcggccgg tgtgccaacc cgcacgcgca
    17161 atgtcacggc aatattgctt aacctgcaac atccgaccca gagcggtctt tggttgtttg
    17221 actgcggtga aggcacccag catcagctac tgcataccgc ctttaaccct ggaaaactgg
    17281 acaagatttt tatcagtcac cttcatggcg atcatctttt tggtttaccc ggcttgctgt
    17341 gcagtcgttc tatgtcaggc attatccaac ccttaacgat ttatggtccg caaggtatcc
    17401 gtgaatttgt ggaaaccgcg ctgcggatta gcggctcatg gaccgattat ccgctggaaa
    17461 ttgtcgaaat tggcgctggc gaaattctcg atgatggcct gcgcaaagta accgcttatc
    17521 cgctggaaca cccactggaa tgttatggct atcgtattga agaacatgat aaaccgggtg
    17581 cattaaatgc ccaggcatta aaagctgctg gcgtgccgcc tggcccactg tttcaggaat
    17641 taaaagcggg caaaacaatc acgctggaag atggaaggca gattaacggc gcagattact
    17701 tagctgctcc agtgccaggt aaagcgctcg ctattttcgg cgataccggc ccctgcgatg
    17761 ccgcacttga cctggctaaa ggtgtcgatg tcatggtgca cgaagcgacg ctggatataa
    17821 ccatggaagc caaagccaat agtcgcggcc atagctctac acgccaggct gcgacactag
    17881 cccgtgaggc tggagtcggt aagctaatca ttacccacgt cagctcgcgc tatgatgaca
    17941 aaggttgtca gcacctgtta cgtgaatgca ggtcaatttt cccggcgact gaactagcga
    18001 atgatttcac cgtgtttaac gtttaactct gcttattatt aaaaacaggg cgaaacttgc
    18061 cctgttatcg caacccgcgc tacatatatc cacccacacg ggcatattga gcgatttttg
    18121 atatttattc atctccttac gcactatatt cagagcaata agttgcctct ttttatcttc
    18181 attcagcaca acctttgcaa ctaaaaggag cccatgatgg ttacagttgt cagcaattat
    18241 tgtcaattat ctcaaaccca actcagtcag acatttgcag aaaaatttac tgtgaccgag
    18301 gaattactgc agtctttaaa aaaaacagcg ttatccggag atgaagaaag tattgagtta
    18361 ctgcataata ttgcgttagg ttatgatgaa tttggaaaaa aagctgaaga tattctttac
    18421 catattgtta gaaacccaac aaatgagacc ctatcgatta tccgacttat taaaaatgcc
    18481 tgtttaaaat tatataattt ggcacatacc gcaaccaact cccaccttaa accaactggt
    18541 ccggataatt cagatgttct cctgttcaaa aagctattct ccccttcgaa attaatgaca
    18601 attatcggtg atgaaattcc tcttatatct gaaaaacagt cgctttcaaa ggtgctttta
    18661 aatgatgaga ataatgaact gagtgatgat acaaacttct gggataaaaa tcgtcaatta
    18721 accacagatg aaatagcttg ctatcttcag aagatcgccg ctaatgcaaa aaatactcaa
    18781 gtcaattatc ctactggtct ctacgtcccc tactccacca gaactcacct ggaagacgct
    18841 ctcaatgaaa atattaagag cgatccatca tggccgaatg aagtccagtt attccacata
    18901 aatactggcg gacactggat attagtttcg ctacagaaaa tagtaaataa aaaaaataat
    18961 aaactacaaa taaaatgcgt catattcaac tcattgcgtg cactaggcca tgataaagaa
    19021 aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggaga aatgtcgaat
    19081 aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc cgatcttcag
    19141 caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga agtgattgaa
    19201 caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta tgctgacaga
    19261 tttaaaaaat attcagcaga agagcagtac gaaattgatt ttcaacatcg actggtaaac
    19321 agaaattgtt atttagataa atatggcgat gcacgaatca atgcttctta tacacaatta
    19381 gaaataaaac actcacaacc caaaaataga gcatccggca aaagagtgag ttaacatcat
    19441 tgttaatcaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt ttgctaaaca
    19501 gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcaggcga ataaattccg
    19561 ccctgtaacc ctgtggatct tccccttttg cctgctgagc ccactgtttg atctgctgcc
    19621 aggaggtatt gttcaggtat tcagaaccgc gtaacttttg cccatatgca gctactgctg
    19681 cgcaaaaacg catatcttca gagggggcgt ttattgttgg ccccagcggg aattcaacta
    19741 actgactttc ttttccctgc gggtatttcc agcgaatctt taaccaggcc agttctttcg
    19801 ttttgtccga tttcactgat ttgttatccg gggcatagcg taacttatca attgatgctt
    19861 tttgcccgtt cagcgttaat tcgaataaca acgttatatg tttgcctgcg ccaatatccc
    19921 ccgcatcaac gttgtcgtta ttaaaatctt ccgcccgaag ttggcgcttt tcataaccaa
    19981 tctgacggta ttccgttacc cacgcggggt taaactcaat ttgcgctttg acatcttttg
    20041 ctacggtaat caacgtctgc cgcatttcac tattcaatac tttctgcgct tcagagaggg
    20101 tatcaatgta gctgtagttg ccgttaccaa catcggcaat tcgcaccatc attgcctcgt
    20161 tgtaattgct attccccacg ccaaacgtcg acagagtaac accagactcc cgctgttttt
    20221 tgaccattga ttcaatcgat tttggatcgt caatgccaac gttaaagtca ccgtcagtgg
    20281 ctaataaaat acgattgatg ccgcccttaa taaacccctt cgccgcctgc tgataagcca
    20341 gttccagccc ggcaccgcca ttggtactgc cttcggcatc cagcgaatca attgcggcat
    20401 taatttccgc cttgtgacta ccggagatag aaggcaacgc aatacgggag tcgccagcgt
    20461 aggtcacgat ggcaatgtta tcctgctcac gaagttcttt gaccaataat ttcaacgaag
    20521 actggataag tggcaaacgt tcatcagaaa tcattgaacc agaagtgtcg ataagaaaga
    20581 ccagattaga agctggtaac tcttcacttt tatgatcttt cgccaggata tcaattttca
    20641 gcaatgttag ctgttcattc cacggcgcag gtgccagttc gtagcgcata gcgaaaggta
    20701 ttggcttagt ggccggaata gattgtttgt ctttgatatc ccagtcatac gggaaataat
    20761 tgactatctc ctccacccgc acagcgtctg gcggaggtaa cagcccatga ttgagaaaac
    20821 ggcgtacatt cgcataactg ccggtgtcga cgtcaaggct aaacgtcgcc aacgggtttt
    20881 gcgctacctg cttaaccgga ttatcatcga actgcttgta gcgagcggtt cctggatttg
    20941 cgatttggga cgctgctttt tctctggctg catgttgata ttttggagcc gcctgtaagc
    21001 gcccctgcaa agcctgtttg tctgaatatt gttgcgcttc ttgctgggct aaggcttttg
    21061 ctttcgcgtc tgcagtggct ttatcggcga cggatctttg ctcggcctct tttattgcag
    21121 cttgttgcgc ggcaagcact tgctggtctg tgggggtact gagctgttgt tgctgacttt
    21181 ccttattctc agattccggc ccgcatcctg acaaaatcaa actactcata agcaacatga
    21241 ttatattttt atttcgcata taaaatcctt ttaaataaat acaggcacca gacataaaat
    21301 atactctttg tataatcctg ctaatcttaa taagatgcaa tatcgaaacc cataacaaat
    21361 aat
//
LOCUS       DAPHJE010000077        21347 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPHJE010000077 DAPHJE010000000
VERSION     DAPHJE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.077"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            86..1375
                     /locus_tag="RH222_002933"
     CDS             86..1375
                     /locus_tag="RH222_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDV3797922.1"
                     /translation="MKGTIFAVALNHRSQLDAWQDAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFAMLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIAYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1372..2838
                     /gene="hpaE"
                     /locus_tag="RH222_002934"
     CDS             1372..2838
                     /gene="hpaE"
                     /locus_tag="RH222_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3797923.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPTDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLVGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2840..3691
                     /gene="hpaD"
                     /locus_tag="RH222_002935"
     CDS             2840..3691
                     /gene="hpaD"
                     /locus_tag="RH222_002935"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDV3797924.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3701..4081
                     /locus_tag="RH222_002936"
     CDS             3701..4081
                     /locus_tag="RH222_002936"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDV3797925.1"
                     /translation="MPHFIVECTENIRQEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4149..4952
                     /gene="hpaH"
                     /locus_tag="RH222_002937"
     CDS             4149..4952
                     /gene="hpaH"
                     /locus_tag="RH222_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDV3797926.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4963..5751
                     /gene="hpaI"
                     /locus_tag="RH222_002938"
     CDS             4963..5751
                     /gene="hpaI"
                     /locus_tag="RH222_002938"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDV3797927.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANELL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPGVY"
     gene            5875..7251
                     /gene="hpaX"
                     /locus_tag="RH222_002939"
     CDS             5875..7251
                     /gene="hpaX"
                     /locus_tag="RH222_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDV3797928.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7261..8151
                     /gene="hpaA"
                     /locus_tag="RH222_002940"
     CDS             7261..8151
                     /gene="hpaA"
                     /locus_tag="RH222_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDV3797929.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8402..9964
                     /gene="hpaB"
                     /locus_tag="RH222_002941"
     CDS             8402..9964
                     /gene="hpaB"
                     /locus_tag="RH222_002941"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDV3797930.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQNSGNMDKMMAMVDRCLSEYDQDGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9982..10494
                     /locus_tag="RH222_002942"
     CDS             9982..10494
                     /locus_tag="RH222_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDV3797931.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10887..13037
                     /gene="btsT"
                     /locus_tag="RH222_002943"
     CDS             10887..13037
                     /gene="btsT"
                     /locus_tag="RH222_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV3797932.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13087..13290
                     /locus_tag="RH222_002944"
     CDS             13087..13290
                     /locus_tag="RH222_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV3797933.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13301..14257
                     /gene="yjiA"
                     /locus_tag="RH222_002945"
     CDS             13301..14257
                     /gene="yjiA"
                     /locus_tag="RH222_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV3797934.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14303..15217)
                     /gene="mrr"
                     /locus_tag="RH222_002946"
     CDS             complement(14303..15217)
                     /gene="mrr"
                     /locus_tag="RH222_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine/cytosine-specific restriction
                     endonuclease Mrr"
                     /protein_id="HDV3797935.1"
                     /translation="MTVPTYDKFIEPVLRYLATKPEGAAARDVHEAAADALGLDDSQR
                     AKVITSGQLVYKNRAGWAHDRLKRAGLSQSLSRGKWCLTPAGFDWVASHPQPMTEQET
                     NHLAFDFVNVKLKSRPDAVDLDPKADSPDHEELAKSSPDDRLDQALKELRDAVADEVL
                     ENLLQVSPSRFEVIVLDVLHRLGYGGHRDDLQRVGGTGDGGIDGVISLDKLGLEKVYV
                     QAKRWQNTVGRPELQAFYGALAGQKAKRGVFITTSGFTSQARDFAQSVEGMVLVDGER
                     LVHLMIENEVGVSSRLLKVPKLDMDYFE"
     gene            15405..18917
                     /gene="hsdR"
                     /locus_tag="RH222_002947"
     CDS             15405..18917
                     /gene="hsdR"
                     /locus_tag="RH222_002947"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     endonuclease"
                     /protein_id="HDV3797936.1"
                     /translation="MMNKSNFEFLKGVNDFTYAIACAAENNYPDDPNTTLIKMRMFGE
                     ATAKHLGLLLNIPPCENQHDLLRELGKIAFVDDNILSVFHKLRRIGNQAVHEYHNDLD
                     DAQMCLRLGFRLAVWYYRLVTKDYDFPVPVFVLPERGENLYHQEVLTLKQQLEQQVRE
                     KAQSQAEVEAQQQKLVALNGYIAILEGKQQETEAQTQARLAALEAQLAEKNAELAKQT
                     EQERKAYHKEITDQAIKRTLNLSEEESRFLIDAQLRKAGWQADSKTLRFSKGARPEPG
                     VNKAIAEWPTGKDETGNQGFADYVLFVGLKPIAVVEAKRKNIDVPGKLNESYRYSKCF
                     DNGFLRETLLEHYSPDEVHEAVPEYETSWQDTSGQQRFKIPFCYSTNGREYRAAMKIK
                     SGIWYRDVRDTRNMSKALPEWHRPEELLEMLGSEPQKQNQWFADNPGMSELGLRYYQE
                     DAVRAVEKAIVKGQQEILLAMATGTGKTRTAIAMMFRLIQSQRFKRILFLVDRRSLGE
                     QALGAFEDTRINGDTFNSIFDIKGLTDKFPEDSTKIHVATVQSLVKRTLQSDEPMPVA
                     RYDCIVVDEAHRGYILDKEQTEGELQFRSQLDYVSAYRRILDHFDAVKIALTATPALH
                     TVQIFGEPVYRYTYRTAVIDGFLIDQDPPIQITTRNAQEGVYLSKGEQVERISPQGEV
                     INDTLEDDQDFEVADFNRGLVIPAFNRAVCNELTNYLDPTGSQKTLVFCVTNAHADMV
                     VEELRTAFKKKYPQLEHDAIIKITGDADKDARKVQTMITRFNKERLPNIVVTVDLLTT
                     GVDIPSICNIVFLRKVRSRILYEQMKGRATRLCPEVNKTSFKIFDCVDIYSTLESVDT
                     MRPVVVRPKVELQTLVNEITDSETYKITEADGRSFAEHSHEQLVAKLQRIIGLATFNR
                     DRSETIDKQVRRLDELCQDAAGVNFNGFASRLREKGPHWSAEVFNKLPGFIARLEKLK
                     TDINNLNDAPIFLDIDDEVVSVKSLYGDYDTPQDFLEAFDSLVQRSPNAQPALQAVIN
                     RPRDLTRKGLVELQEWFDRQHFEESSLRKAWQETRNEDIAARLIGHIRRAAVGDALKP
                     FEERVDHALTRIKGENDWSSEQLSWLDRLAQALKEKVVLDDDVFKTGNFHRRGGKAML
                     QRTFDDNLDNLLDKFSDYIWDELA"
     gene            19118..20707
                     /gene="hsdM"
                     /locus_tag="RH222_002948"
     CDS             19118..20707
                     /gene="hsdM"
                     /locus_tag="RH222_002948"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system
                     methyltransferase"
                     /protein_id="HDV3797937.1"
                     /translation="MNNNDLVAKLWKLCDNLRDGGVSYQNYVNELASLLFLKMCKETG
                     QEAEYLPEGYRWDDLKSRIGQEQLQFYRNLLVHLGADNQKLVQAVFQNVNTTITQPKQ
                     LTELVSNMDSLDWYNGAHGKSRDDFGDMYEGLLQKNANETKSGAGQYFTPRPLIKTII
                     HLLKPQPREVVQDPAAGTAGFLIEADRYVKSQTNDLDDLDGDTQDFQIHRAFIGLELV
                     PGTRRLALMNCLLHDIEGNLDHGGAIRLGNTLGSDGENLPKAHIVATNPPFGSAAGTN
                     ITRTFVHPTSNKQLCFMQHIIETLHPGGRAAVVVPDNVLFEGGKGTDIRRDLMDKCHL
                     HTILRLPTGIFYAQGVKTNVLFFTKGTVANPHQDKNCTDDVWVYDLRTNMPSFGKRTP
                     FTDEHLQPFERVYGEDPHGLSPRSEGEWSFNAEETEVADSEENKNTDQHLATSRWRKF
                     TREWIRTTKSDSLDISWLKDKDSIDADNLPEPDVLAAEAMGELVQALGELDALIRELG
                     ASDEADAQRQLLEEAFGGVKE"
     gene            20704..>21347
                     /locus_tag="RH222_002949"
     CDS             20704..>21347
                     /locus_tag="RH222_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV3797938.1"
                     /translation="MSFNSTSKELIEQNINGLLSIHDSWLRISMDSVANITNGFAFKS
                     SEFNNRKDGVPLIRIRDVLKGNTSTYYSGQIPEGYWVYPEDLIVGMDGDFNATIWCSE
                     PALLNQRVCKIEVQEDKYNKRFFYHALPGYLSAINANTSSVTVKHLSSRTLQDTLLPL
                     PPLAEQKIIAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLAAAVTGRLTKED"
BASE COUNT         5134 a         5805 c         5753 g         4655 t
ORIGIN      
        1 cttacttttg ccaaactacg caatcgggaa taaaagtaat cattaacata ttaatgatta
       61 agatgatcga atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca
      121 tcgcagccag cttgatgcat ggcaggacgc gttccagcaa tccccctaca aagccccgcc
      181 taaaactgcg gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat
      241 tccctttcca cagggtgaaa aggtactgag cggtgcgaca gttgcgttga ttgtgggaaa
      301 aacggcgacg aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc
      361 taacgacgtc agcctgccag aagagagctt ttaccgcccg gcaatcaaag caaaatgccg
      421 tgatggattc tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgactat
      481 ctataccgag atcaacgggc gtcctgccga tcactggaac accgccgatt tacaacgtaa
      541 cgccgcacag ttgctgagtg ccctgagcga atttgccatg ctgaatccag gcgatgccat
      601 tctgctcggc acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg tgcgtgttct
      661 cgcagaaggt ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgcg
      721 caagagcttc ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta
      781 cgccgaccac gccagcgaac tggaatttaa gccaccggaa gagccgctgg tgttcctgaa
      841 agcgccgaat accctcactg gcgataacca gacctccgtt cgcccaaaca atattgcata
      901 catgcactac gaagcggagc tggtggtggt gattggcaag caggcgcgta acgtcagcga
      961 agccgatgcc atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgcga
     1021 ctatctggaa aactactacc gccctaacct gcgggtaaaa agccgcgacg gactgacgcc
     1081 gatgctttca accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg
     1141 caccttcgtc aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt
     1201 gcccttcctg atcgcctact taagcgaatt tatgaccctg aatccgggcg acatgatcgc
     1261 caccggcaca ccaaaaggct tatctgacgt agtgcctggc gatgaagtag tggtggaagt
     1321 agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag gaaacagcga aatgaaaaaa
     1381 gtaaatcatt ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gaccaccaat
     1441 ccggcaacgg gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag
     1501 gcggtagcgg cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgt
     1561 gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga acgtgccaga gatcgccgcg
     1621 atggaaaccg cggacaccgg cctgccgatc catcagacca aaaatgtgtt gatcccacgc
     1681 gcttctcaca actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat
     1741 ccggttgacg acaagatgct caactacacg ctggtgcaac cggtaggcgt ttgtgcgctg
     1801 gtatcgccgt ggaacgtgcc gtttatgacc gccacatgga aggtcgcgcc gtgtcttgcg
     1861 ctgggcaata ccgcggtgct gaagatgtcg gaactctccc cgctgaccgc tgaccgcctg
     1921 ggtgagctgg cgctggaagc cggtattccg gcgggcgtgc tgaacgtggt acagggctac
     1981 ggcgcaaccg caggggatgc gctggttcgt catcatgacg tacgtgccgt gtcgttcacc
     2041 ggcggtacgg ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg
     2101 gaactgggcg gtaaatcgcc ggtgctgatt tttgaagatg ccgatattga acgtgcgctg
     2161 gacgctgccc tgttcaccat cttctcaatc aacggcgaac gctgcaccgc cggttcgcgc
     2221 atttttattc aacagagcat ctatccagaa ttcgttaaac gctttgccga acgcgccaac
     2281 cgtctgcgcg tgggcgatcc gaccgatccg aatacccagg ttggggcgct tatcagccag
     2341 cagcactggg aaaaagtctc cggctatatc cgtctaggca ttgaagaagg cgcaaccctg
     2401 ctggtgggcg gtccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc
     2461 ctgcgcccaa cggtgctggc ggacgtagat aaccgtatgc gcgttgccca ggaagagatt
     2521 ttcgggccgg tcgcctgcct gctgccgttt aaagatgaag ccgaaggctt acgcctggca
     2581 aacgacgtgg agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta
     2641 cgcctggcgc gtggcattga agcaggcatg gtgttcgtca acacccagaa cgtgcgcgac
     2701 ctgcgccagc cgtttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac
     2761 agcttcgaag tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt
     2821 ccaaaatggg gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc
     2881 gtcgatgtat ctctctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga
     2941 cgggcataaa gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt
     3001 cgacacccac tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg
     3061 cgtctacacc agcaacgagt tgccgcattt tattcgcgac atgacctaca actatgaggg
     3121 caacccggag ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa
     3181 agcgcacaac attcccagcc tgaaactgga atacggcacg ctggtgccaa tgcgctacat
     3241 gaatgaagac aagcacttca aagtggtctc catctccgca ttctgtaccg ttcacgattt
     3301 cgccgacagc cgcaagctgg gcgaagcgat tctgaaagcg atcgaacagt acgacggcac
     3361 cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgtgcgga
     3421 agaagggatg aacagctaca cccgcgagtt cgaccgccag atggacgagc gcgtggtgaa
     3481 gttgtggcgc gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta
     3541 ctgctacggc gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcgggtggga
     3601 taaatatgac ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca
     3661 ggttaacgct gttttcccgc tgcccgcgta aggaaccacc atgccgcact ttatcgtgga
     3721 atgtactgaa aatattcgtc aagaagcccg cttacctgag ctgtttgcca gtgttaacac
     3781 tgcactggcg gcgacgggga tttttccgct tgggggaata cgtagccggg cgcactggat
     3841 cgatacctgg caaatggctg atggcaagca cgattacgcc tttgtgcata tgacgctaaa
     3901 aatcggcagc ggacgcagtc tggaaagccg tcaggaagtg ggcgaaatgc tgtttgatct
     3961 gattaaaacg cacttcgcct cactgatgga gagccgttac ctggcgcttt catttgagat
     4021 tgcagagctg cacccgacgt tgaatttcaa acagaacaac gtacacgcgt tgtttaaata
     4081 aatttgtacc agcttccagg ccggatgata aggcgcagcc gcatccggca atgccacagg
     4141 atatcgctat gttcgacaaa cacacccaca ccctgatcgc ccagcgtctg gatcaggcag
     4201 aaaaacagcg cgaacagatc cgcgcgatct cgctggatta cccggagatc accatcgaag
     4261 acgcttacgc ggtgcagcgt gaatgggttc gactgaaaat cgccgaaggt cgcacgctga
     4321 aaggccacaa aatcggcctg acctcgaaag cgatgcaggc cagctcgcag atcagcgaac
     4381 cggattacgg tgcactgctg gacgacatgt tcttccacga tggcagcgat atcccgaccg
     4441 atcgctttat cgtgccgcgc attgaagtgg agctggcttt tgtgctggca aaaccgctgc
     4501 gtggaccaaa ctgcacgctg ttcgacgttt acaacgccac ggactatgtg atcccggcgc
     4561 tggagctgat cgacgctcgc tgccacaaca tcgatccgga aacccagcgc ccgcgtaaag
     4621 tgttcgacac catttctgat aacgccgcca atgccggggt gatcctcggt ggtcgtccca
     4681 ttaagcccga tgagttggat ctacgttgga tctccgccct gatgtatcgc aatggcgtga
     4741 ttgaagaaac cggcgtcgcc gctggcgtgc tgaatcatcc ggcaaacggc gtggcctggc
     4801 tggcgaacaa actcgccccc tatgacgtac aactggaagc cgggcaaatc attctcggcg
     4861 gttcgttcac ccgcccggtt ccggcgcgta agggcgacac cttccacgtc gattacggca
     4921 acatgggctc cattagctgc cgctttgttt aaggagagaa cgatggaaaa cagttttaaa
     4981 gcggcgctga aagcaggccg tccgcagatt ggattatggc tggggctgag cagcagctac
     5041 agcgcggagt tactggccgg agcaggattc gactggttgt tgatcgacgg tgagcacgca
     5101 ccgaacaacg tacaaaccgt gctcacccag ctacaggcga ttgcgcccta tcccagccag
     5161 ccggtagtac gtccgtcgtg gaacgatccg gtgcaaatca aacaactgct ggacgtcggc
     5221 acacaaacct tactggtgcc gatggtacaa aacgccgacg aagcccgtga agcggtacgc
     5281 gccacccgtt atccccccgc cggtattcgc ggtgtgggca gtgcgctggc tcgcgcctcg
     5341 cgctggaatc gcattcctga ttacctgcaa aaagccaacg atcaaatgtg cgtgctggtg
     5401 cagatcgaaa cgcgtgaggc aatgaagaac ttaccgcaga ttctggacgt ggaaggcgtc
     5461 gacggcgtgt ttatcggccc ggcggatctg agcgccgata tgggttatgc cggtaatccg
     5521 cagcacccgg aagtacaggc cgccattgag caggcgatcg tgcagatccg cgaagcgggc
     5581 aaagcgccgg ggatcctgat cgccaatgag ctactggcaa aacgctatct ggaactgggc
     5641 gcgctgtttg tcgccgtcgg cgttgacacc accctgctcg cccgcgccgc cgaagcgctg
     5701 gcagcacggt ttggcgcgca ggctacagcg attaagcccg gcgtgtatta atgccggatg
     5761 cgctgcactt atccggccta cactcgcacc aaaccgtagg ccggataagg cgtttacgcc
     5821 gcatccgcca taaaccttgt cgtaccctac aaaaatccca ttagaggaag aaaaatgagc
     5881 gacacctcac ctgccatacc ggagagtatc gatccggcga atcagcataa agcgctgact
     5941 gccggacaac aggcggttat taagaagcta tttcgccgcc tgatcgtctt tctgttcgtg
     6001 ctgtttatct tctcgttcct tgatcgcatc aacatcggct ttgccggact cacgatggga
     6061 cgcgacctcg gtctgagcgc caccatgttt ggcctcgcta ccaccctgtt ctacgccgct
     6121 tatgtcatct tcggcattcc cagcaacatt atgctgagta ttgtcggtgc acggcgctgg
     6181 atcgccacca tcatggtgct ctggggcatc gcctctactg ctaccatgtt tgccactggc
     6241 cccaccagct tatacgtact gcgtatactg gttggcatta ccgaagccgg ctttctgcct
     6301 ggcattctgc tctatttaac cttctggttt ccggcctact tccgcgcccg tgccaacgcc
     6361 ttgtttatgg tggcaatgcc ggtaacgaca gcgttgggat cgatcgtttc cggctacatt
     6421 ttgtcgctgg atggcgtaat ggcattaaaa ggctggcagt ggctgttttt gctggaaggc
     6481 ttcccgtcgg tattactcgg cgtcatggtg tggttctggc ttgatgactc accggacaaa
     6541 gctaagtggc tgacgaaaga agacaaaaaa tgcctgcaag agatgatgga taacgatcgt
     6601 ctgacgctgg ttcagccaga gggagccatc agccaccatg ccatgcaaca acgcagcatg
     6661 tggcgggaga tcttcactcc ggtggtgatg atgtataccc tggcgtattt ctgcctgacc
     6721 aacacactta gtgcgatcag catctggaca ccgcagatcc tgcaaagctt taatcagggc
     6781 agcagtaata tcaccatcgg cctgctggcc gccgtaccgc agatttgtac cattctcggg
     6841 atgatctact ggagccgtca ctcagatcgc cgccaggaac gaaggcatca caccgccctt
     6901 ccttatttgt tcgctgccgc tggttggtta ctggcttcgg caactgatca caacatgatc
     6961 cagatgctgg ggatcattat ggcttcgacc ggatcattca gcgcaatggc gattttctgg
     7021 acaacaccgg atcagtccat cagcctgcgg gcacgagcga tcggtattgc ggtgatcaac
     7081 gccactggca acattggttc agcattaagt ccgtttatga tcggctggtt gaaagatctg
     7141 accggcagct ttaacagtgg attgtggttt gttgccgcgc tgctggtgat tggtgcaggg
     7201 attatctggg caattccaat gcagtcctcc cgtccgcgag cgaccccgta aggaacgacg
     7261 atgtgtgacc gtcagattgc caatattgat atcagcaaag agtacgatga aagcctgggc
     7321 acggacgatg tgcattatca gtcgttcgcc cgtatggcgg ccttttttgg ccgccatatg
     7381 ctgccacatc gccacgaaca gtactttcag atgcatttcc tcaatagcgg acagattgag
     7441 ctacagcttg acgatcatcg ctactcggtg gaagcgcccc tgtttgtcct gacgccgccg
     7501 tcagtacctc atgcgtttat tacggagtct gacgccgacg gtcatgtgtt gacggtacgg
     7561 gaagatctga tctggcccct gctggaagtt ctttatccag gcactcggga aaccttcggc
     7621 ctgccgggga tttgcctgtc actggcagat aaacccgacg aactggcggc gctggaacac
     7681 tattggcaac tgatagagcg ggaatcggta gaacaactgc ctggtcggga acacaccctg
     7741 acgctcctgg cacaggcagt gttcacccta ctgctgcgta acgcaaaact tgacgatcat
     7801 gccgccagcg gaatgcgcgg agaattaaaa ctgttccagc gttttaatat gctgattgaa
     7861 agccattttc atcagcactg gacagtaccg gattacgcta acgaactgca tatcaccgaa
     7921 tcccgcctca cggacatctg ccgccgcttt gccaaccgtc cgccaaaacg gttgattttc
     7981 gacaggcagc tgcgagaagc caagcggctg ctgctgtttt ctgataacgc cgtgaacaat
     8041 attgcctggc aactcggttt taaggatccg gcttattttg cgcgcttttt taatcgttta
     8101 gtcggttgct cgcccagtgc ttatcgtgcc aaaaaagtac ctgtgacgtg aaaatccctt
     8161 agccttaacg ggaaaccagg caccacctgc tattcccctt tctccgcctg gagcaagggg
     8221 atttagtaac gttaaaaatc gctaaaagcg acctcgatca caaaaccgta cccgacctga
     8281 aaagtaccag ccattgtccc aaaagtgtct tcccgtaatc gcccctgtaa gcttcaattg
     8341 aataacaaaa acaacacaca aaattaacaa tcgtattccg attaatactg tagaggtcga
     8401 catgaaacca gaagatttcc gcgccagtac ccaacgtcct ttcaccgggg aagagtatct
     8461 gaaaagcctg caggatggtc gcgagatcta tatctatggc gagcgagtga aagacgtcac
     8521 cactcatccg gcatttcgta atgcggcagc gtctgttgcc cagctgtacg acgcactgca
     8581 caaaccggag atgcaggact ctctgtgttg gaacaccgac accggcagcg gcggctatac
     8641 ccataaattc ttccgcgtgg cgaaaagtgc cgacgacctg cgccagcaac gcgacgccat
     8701 cgctgagtgg tcacgcctga gctatggctg gatgggccgt accccagact acaaagccgc
     8761 tttcggttgc gcactgggcg cgaatccggg cttttacggt cagttcgagc agaacgcccg
     8821 taactggtac acccgtattc aggaaactgg cctctacttt aaccacgcga ttgttaaccc
     8881 accgatcgat cgtcatttgc cgaccgataa agtgaaagac gtttacatca agctggaaaa
     8941 agagactgac gccgggatta tcgtcagcgg tgcgaaagtg gttgccacca actcggcgct
     9001 gactcactac aacatgattg gcttcggctc ggcacaagtg atgggcgaaa acccggactt
     9061 cgcactgatg ttcgttgcgc caatggatgc cgatggcgtg aaattaatct cccgcgcctc
     9121 ttatgagatg gtcgcgggtg ctaccggctc gccatacgac tacccgctct ccagccgctt
     9181 cgatgagaac gatgcgattc tggtgatgga taacgtgctg attccatggg aaaacgtgct
     9241 gatctaccgc gattttgatc gctgccgtcg ctggacgatg gaaggcggtt ttgcccgtat
     9301 gtatccgctg caagcctgtg tgcgcctggc agtgaaatta gacttcatta cggcactgct
     9361 gaaaaaatca ctcgaatgta ccggcaccct ggagttccgt ggtgtgcagg ccgatctcgg
     9421 tgaagtggta gcgtggcgca acaccttctg ggcattgagt gactcgatgt gttcagaagc
     9481 aacgccgtgg gtcaacgggg cttatttacc ggatcatgcc gcactgcaaa cctatcgcgt
     9541 actggcacca atggcctacg cgaagatcaa aaacattatc gaacgcaacg ttaccagtgg
     9601 cctgatctat ctcccttcca gtgcccgtga cctgaataat ccgcagatcg accagtatct
     9661 ggcgaagtat gtgcgcggtt cgaacggtat ggatcacgtc cagcgcatca agatcctcaa
     9721 actgatgtgg gatgctattg gcagcgaatt tggtggtcgt cacgaactgt atgaaatcaa
     9781 ctactccggt agccaggatg agattcgcct gcagtgtctg cgccaggcac aaaactccgg
     9841 caatatggac aagatgatgg cgatggttga tcgctgcctg tcggaatacg accaggacgg
     9901 ctggactgtg ccgcacctgc acaacaacga cgatatcaac atgctggata agctgctgaa
     9961 ataacgcagc aggaggttaa gatgcaatta gatgaacaac gcctgcgctt tcgtgacgcg
    10021 atggccagcc tgtcggcagc ggtaaatatt atcaccaccg agggcgacgc cggacaatgc
    10081 gggattacgg caacggccgt ctgctcggtc acggatacac caccgtcgct gatggtgtgc
    10141 attaacgcca acagtgcgat gaacccggtt tttcagggca acggcaagtt gtgcgtcaac
    10201 gtcctcaacc atgagcagga actgatggca cgccacttcg cgggcatgac aggcatggcg
    10261 atggaagagc gttttagcct ctcatgctgg caaaaaggtc cgctggcgca gccggtgcta
    10321 aaaggttcgc tggccagtct tgaaggtgag atccgcgatg tgcaggcaat tggcacacat
    10381 ctggtgtatc tggtggagat taaaaacatc atcctcagtg cagaaggtca tggacttatc
    10441 tactttaaac gccgtttcca tccggtgatg ctggaaatgg aagctgcgat ttaagctcca
    10501 gccccttcac gcattcgtcg aaggggcatt tttattgtga tctcaatcac atatccaccc
    10561 gatgaataat tcttgcccaa ccgctcaccg ccattcctga ccgcttacct ccctaaaata
    10621 accactcagt tatttacctt actttacgcg cgcgtatctc tggcaacatc actgcaggat
    10681 agcggtcaat ttacctcctc aaacgcaacg caaacctaga acggcttcgg cctatgatta
    10741 acaaattact ctatggattt tggtttgcag gaaggcagca agtgagtgaa tccccgggag
    10801 cttacaacag taagtgacag gggtgaacga acgcaactgc cgcacctgta agccaaaaga
    10861 cgacgagtaa aatgccaggt tttactatgg atacgaaaaa actattcaag cacataccct
    10921 gggtgattct cggaatcatc ggtgcattct gcctcgcggt agttgcatta cgtcgggggg
    10981 agcacgtcag cgccctgtgg atcgtggtcg cctctgtgtc ggtgtatctg gtggcgtatc
    11041 gctactacag tttgtatatc gcccagaagg tgatgaaact cgaccccacg cgcgcgacgc
    11101 ctgcggttat taacaacgac ggtctgaact acgttccgac caaccgttac gtgttgtttg
    11161 gtcaccactt cgccgctatc gccggtgccg gtccgctggt tggtccggtt ctcgccgcgc
    11221 agatgggcta cctgcccggc acgctgtggc tgctggcggg ggtagtgctg gccggtgcgg
    11281 tacaggactt tatggtgctg tttatctcct ctcgccgtaa cggcgcatct ctcggtgaga
    11341 tgatcaaaga agagatgggg ccagtaccgg gaactatcgc gctgtttggc tgtttcttaa
    11401 tcatgatcat catcctcgcc gtcctggcgc tgattgtggt taaagccctg gccgaaagtc
    11461 cgtggggtgt cttcaccgtt tgctcaaccg taccgattgc gctgtttatg ggtatctaca
    11521 tgcgctttat ccgtccgggg cgtgtgggtg aagtctctgt cattggtatc gtgctgctgg
    11581 ttgcctctat ctacttcggt ggcgtgattg ctcacgatcc gtactggggt ccggcactga
    11641 cctttaaaga caccaccatt accttcgcgc tgattggcta tgcgtttgtt tccgcgctgc
    11701 tgccggtgtg gctgatcctc gcaccgcgcg actatctggc aaccttcctg aaaatcggcg
    11761 ttatcgtcgg cctggcgctg ggtatcgtgg tgctgaaccc ggaactgaaa atgcctgcga
    11821 tgacccagta cattgacggt actggcccgc tgtggaaagg cgctctgttc ccattcctgt
    11881 tcatcaccat cgcctgtggt gcggtatctg gcttccacgc gctgatctct tccggtacga
    11941 cgccgaaact gctggctaac gaaaccgacg cgcgtttcat tggctacggc gcaatgctga
    12001 tggagtcctt cgtggcgatt atggcgctgg ttgctgcgtc catcatcgaa ccgggtcttt
    12061 acttcgcgat gaacaccccg ccagcgggtc ttggcatcac catgcctaac ctgcatgaaa
    12121 tgggtggcga gaacgcgccg atcatcatgg cgcagctgaa agacgttacc gcacacgcgg
    12181 cggcgaccgt cagctcctgg ggcttcgtga tttcgccaga gcagatcctg caaaccgcga
    12241 aagacattgg tgagccttct gtcctgaacc gtgcaggtgg tgcgccaacg ctggcggtag
    12301 gtatcgctca cgtgttccac aaagtgctgc cgatggctga catgggcttc tggtatcact
    12361 tcggtattct gttcgaagcc ctgttcatcc tgaccgcgct ggatgcgggt acccgttctg
    12421 gccgctttat gctgcaagac ctgctgggta acttcatccc gttcctgaaa aaaaccgact
    12481 ctctggttgc cggtatcatc ggtactgcgg gctgtgtggg tctgtggggc tacctgctgt
    12541 atcagggcgt ggtcgatccg ctgggcggcg ttaagagcct gtggccgctg ttcggtattt
    12601 ccaaccagat gctggcagcc gtagcgctgg tactgggcac cgttgtgctg attaagatga
    12661 agcgcaccca atacatctgg gtaactgttg ttccggctgt atggctgctt atctgcacca
    12721 cctgggcgct gggtctgaaa ctgttcagca ccaacccgca gatggaaggc ttcttctaca
    12781 tggcaagcca gtacaaagag aagattgcta acggtactga cctgacggcg cagcagattg
    12841 ccaacatgaa ccacatcgtt gtgaacaact acaccaacgc aggcctgagt attctgttcc
    12901 tgattgtggt gtacagcatc atcttctacg gtttcaaaac ctggcttgcg gtgcgtaaca
    12961 gcgacaaacg tactgacaaa gaaacaccgt acgttccaat cccggaaggc ggcgtgaaga
    13021 tctcttcgca ccactaaccg tgtttagccc cgcttcggcg gggctttgtt cttatcagag
    13081 tgaactatgt ttggtaactt aggacaggca aaaaaatatc tcggccaggc ggcgaagatg
    13141 ttgattggca ttccagacta cgacaactac gtcgagcata tgaagaccaa ccatcccgac
    13201 aagccgtaca tgagctatga agaattcttc cgcgaacgcc agaacgcgcg ctacggcggc
    13261 gatggtaaag gcggtatgcg ctgttgttaa tggagagagc atgaacccga ttgcagttac
    13321 cctactcacc ggttttttag gcgcgggcaa aaccaccctg ctgcgccata ttcttaatga
    13381 acaacacggc tacaagattg ccgtgattga aaacgaattc ggcgaagtct ccgttgatga
    13441 tcaactgatt ggcgaccgcg ccacgcagat caaaacgctg accaacggct gcatctgctg
    13501 ttcgcgctcc aacgagctgg aagacgcact actcgacctg ctggataatc tcgacaaggg
    13561 caatattcag ttcgatcgtc tggtcattga atgcaccggc atggccgatc ctggcccgat
    13621 tattcagacc tttttctccc atgagatttt atgccagcgt tacctgctgg acggcgtgat
    13681 tgcgctggtg gatgcggtac atgccgatga gcagatgaac cagttcacca tcgcccagtc
    13741 ccaggttggc tacgccgacc gtattctgct gactaaaacc gacgtcgcag gcgaagcaga
    13801 gaaactgcgt gaacgtctgg cacgcatcaa tgcccgcgca ccggtctaca ccgtcaccca
    13861 cggcgacatc gacctgggtc tgctgttcaa caccaacggt tttatgctgg aagagaatgt
    13921 cgtcagcacc aaaccgcgtt tccactttat cgcggacaaa caaaacgata tttcgtcgat
    13981 tgtggtggaa ctggattacc cggtagatat cagcgaagtt tcccgggtga tggaaaacct
    14041 gctgctggaa tcggcagata aactgctgcg ttacaaaggg atgctgtgga ttgacggcga
    14101 acctaaccgc ctgctgttcc agggcgtcca gcgcctctac agcgccgact gggaccgccc
    14161 gtggggcgat gaaaaaccgc atagcacaat ggtgtttatc gggattcagt tgccggagga
    14221 agagattcgg gcggcgtttg cggggttgag gaagtaaggg gttatgggcc ggataaggcg
    14281 cagccgcatc cggcctgata tttcactcaa aatagtccat atccagtttc ggcaccttca
    14341 acaaacgtga agaaacccct acttcgtttt cgatcattaa gtgcaccagg cgttccccat
    14401 caaccaacac cataccctcg acggattgag caaagtcacg cgcctgagac gtaaatccag
    14461 aagtggtaat aaacacccca cgtttcgcct tttgcccagc cagtgcgccg taaaatgcct
    14521 gtaattctgg cctgcctaca gtgttctgcc aacgttttgc ctgaacataa actttctcca
    14581 ggccaagttt atcaagcgat atcacaccat cgatgccacc atctccagta ccgccaacac
    14641 gctgcaaatc atcacggtgg ccgccatacc ccaggcgatg caaaacatcc agaacaatga
    14701 cttcaaagcg cgaaggagaa acctgcaata agttttccag aacctcatca gccaccgcat
    14761 cacgaagctc ttttagcgcc tgatctaacc gatcgtccgg gctgctcttt gcaagttctt
    14821 catgatcggg agagtcggct ttcggatcta aatcgacggc atccggccgt gacttaagtt
    14881 tgacattcac aaaatcgaag gccagatggt tcgtctcctg ctccgtcatt ggctggggat
    14941 gagacgcaac ccagtcaaaa cccgcaggag tcaggcacca tttgccacgc gacaaacttt
    15001 gcgacaaccc ggcacgtttt aaacggtcat gcgcccagcc tgcacgattt ttataaacaa
    15061 gttgtccgct ggtaatgact ttcgctcgct ggctgtcatc cagtcctaat gcatccgcgg
    15121 cagcctcatg aacatcacgc gcggctgcac cttccggttt tgtcgccaga taacgcagaa
    15181 caggttcaat aaatttgtca taggtaggaa ccgtcatagt acatccttgc agaatcaggt
    15241 agatgttttt cggctactat aacactacca aaatagacga acacgttaga aatgagtcag
    15301 ttgctgtgac cgtggtcatt gcccggaaag gtacagaaag ctaagataag gcgttatggg
    15361 ccttaaatat ttggacaggc ccacacagca atggattaat aacaatgatg aataaatcca
    15421 attttgaatt cctgaagggc gtcaacgact ttacctatgc catcgcctgt gcggcggaaa
    15481 ataactaccc ggatgatccc aacacgacgc tgattaaaat gcgtatgttt ggcgaagcca
    15541 cagcgaaaca tcttggtctg ttactcaaca tccccccttg tgagaatcaa cacgatctcc
    15601 tgcgcgaact cggcaaaatc gccttcgttg atgacaacat tctctctgta ttccacaaat
    15661 tacgccgcat tggtaaccag gcggtgcacg aatatcataa cgatctcgac gatgcccaga
    15721 tgtgcctgcg actcgggttt cgcctggctg tctggtacta ccgtctggtc actaaagatt
    15781 atgacttccc ggtgccggtg tttgtgttgc cggaacgtgg tgaaaacctc tatcaccagg
    15841 aagtgctgac gctaaaacaa cagcttgaac agcaggtgcg agaaaaagcg cagagtcagg
    15901 cagaagtcga agcacaacag cagaagctgg ttgccctgaa cggctatatc gccattctgg
    15961 aaggcaagca gcaggaaacc gaagcgcaaa cccaggctcg ccttgctgca ctggaagcac
    16021 agctcgccga gaagaacgcg gaactggcca agcagaccga acaggaacgt aaggcttacc
    16081 acaaagaaat taccgatcag gccatcaagc gtacactcaa tcttagcgaa gaagagagcc
    16141 gcttcctgat tgacgcgcaa ctgcgtaaag caggctggca ggccgatagc aaaaccctgc
    16201 gcttctccaa aggcgcgcgc ccggaacccg gcgtcaataa agccattgcc gaatggccga
    16261 ccgggaaaga tgaaacgggt aatcagggct ttgcggatta tgtgctgttt gtcggtctca
    16321 aacccatcgc ggtggtagaa gcgaaacgta aaaatatcga cgttcccggc aagctcaatg
    16381 agtcgtatcg ctacagtaaa tgtttcgata atggcttcct gcgggaaacc ttgcttgagc
    16441 actattcacc agatgaagtg catgaagcgg tgccagagta tgaaaccagc tggcaggaca
    16501 ccagcggcca acaacggttt aaaatcccct tctgctactc aaccaacggg cgcgaatacc
    16561 gcgcagcgat gaagattaaa agcggcatct ggtatcgcga cgtgcgtgat acccgcaata
    16621 tgtcgaaagc cttacccgag tggcaccgcc cggaagagct gctggaaatg ctcggcagcg
    16681 aaccgcaaaa acagaatcag tggtttgccg ataaccctgg catgagtgag ctgggcctgc
    16741 gttattatca ggaagatgcc gtccgcgcgg ttgaaaaggc aatcgtcaag gggcaacaag
    16801 agatcctgct ggcgatggca accggtaccg gtaaaacccg tacggcaatt gccatgatgt
    16861 tccgcctgat ccagtcccag cgttttaaac gcattctctt ccttgtcgac cgccgttctc
    16921 ttggcgaaca ggcgctgggc gcgtttgaag atacgcgtat taacggcgac acctttaaca
    16981 gcattttcga cattaaaggg ctgacggata aattccctga agacagcacc aaaattcacg
    17041 ttgccaccgt acagtcgctg gtgaaacgca ccctgcaatc ggatgaaccg atgccggtag
    17101 cccgttacga ctgtatcgtc gtcgatgaag cgcatcgcgg ctacattctc gataaagagc
    17161 agaccgaagg cgaactacag ttccgcagtc agctggatta cgtctctgcc taccgtcgta
    17221 ttctcgatca cttcgatgcg gtaaaaatcg ctctcaccgc caccccggct ctgcatacgg
    17281 tgcagatttt cggcgagccg gtttaccgtt acacctaccg taccgcggtt atcgacggtt
    17341 ttctgatcga ccaggatccg ccgattcaga tcaccacccg caacgcccag gaaggggttt
    17401 atctctccaa aggcgagcag gttgagcgca tcagcccgca gggggaagtg atcaacgaca
    17461 ccctggaaga cgatcaggat tttgaggtcg ccgactttaa ccgtggcctg gtgatcccgg
    17521 cgtttaaccg cgccgtctgt aacgaactca ccaactacct tgacccgacc gggtcacaaa
    17581 aaacgctggt cttctgcgtc accaatgccc atgccgatat ggtggtggaa gagctgcgta
    17641 ccgcgttcaa gaaaaagtat ccacaactgg agcacgacgc gatcatcaag atcaccggtg
    17701 atgccgataa agacgcgcga aaagtgcaga ccatgatcac ccgcttcaat aaagagcggc
    17761 tgcccaatat cgtggtcacc gtcgacctgc tgacgaccgg cgtcgatatt ccgtcgatct
    17821 gtaatatcgt gttcctgcgc aaggtacgca gccgtattct gtacgagcag atgaaagggc
    17881 gcgccacgcg cttatgcccg gaggtgaata aaaccagctt taagattttc gactgtgtcg
    17941 atatctacag cacgctggag agcgtcgaca ccatgcgtcc ggtggtggtg cgtccgaagg
    18001 tggaactgca aacgctggtc aacgaaatta ccgattcaga aacctataaa atcaccgaag
    18061 cggatggccg cagttttgcc gagcacagcc atgaacaact ggtggcgaag ctccagcgca
    18121 tcatcggcct ggccacgttt aaccgtgacc gcagcgaaac gatagacaaa caggtgcgtc
    18181 gtctggatga gctatgccag gacgcggcgg gggttaactt taacggcttc gcctcgcgcc
    18241 tgcgggaaaa agggccgcac tggagcgccg aagtctttaa caaactacct ggctttatcg
    18301 cccgtctgga aaagctgaaa accgacatca acaacctgaa tgatgcgccg atcttcctcg
    18361 atatcgacga tgaagtggtg agcgtaaaat cgctgtacgg tgattacgac acgccgcagg
    18421 atttcctcga agcctttgac tcgctggtgc aacgttcccc gaatgcacag ccggcattgc
    18481 aggcggtgat taatcgcccg cgcgatctca cccgtaaagg gctggtcgag ctacaggagt
    18541 ggtttgaccg ccagcacttt gaggaatctt ccctgcgcaa agcatggcaa gagacgcgca
    18601 atgaagatat cgccgcccgg ctgattggac atattcgccg cgctgcggtg ggcgatgcgc
    18661 tgaaaccgtt tgaggaacgt gtcgatcacg cgctgacgcg cattaagggc gaaaatgact
    18721 ggagcagcga gcaattaagc tggctcgatc gtttagcgca ggcgctgaaa gagaaagtgg
    18781 tgctcgacga cgatgtcttc aaaaccggca acttccaccg tcgcggcggg aaggcgatgc
    18841 tgcaaagaac ctttgacgat aatctcgaca acctgctcga taaattcagc gattacattt
    18901 gggacgagct ggcctgacac gtatacactt catccttcag gctgcctctg cgttggctgc
    18961 gctcgttcac cccggtcaag tacttctgta cgctcccggg gattcgctca cttgccgcct
    19021 tgatgcaatc tgaatgattt tgtgtatatt accctcggca atttcttctt ctgcggcccc
    19081 gaacatgcgg gccgctgctt aatttacgga acttacaatg aacaataacg atctggtcgc
    19141 gaagctgtgg aaactgtgcg acaacctgcg cgatggcggc gtttcctatc aaaactacgt
    19201 caatgaactc gcctcgctgc tgtttttgaa aatgtgtaaa gaaaccggac aggaagcgga
    19261 atacctgccg gaaggctacc gctgggatga cctgaaatcc cgcatcggcc aggagcagtt
    19321 gcagttctac cgtaacctgc tggtgcatct gggcgccgac aatcaaaagc tggtgcaggc
    19381 ggtgttccag aacgtcaaca ccaccattac ccagccgaaa cagttgaccg aactggtcag
    19441 caatatggat tcactggact ggtacaacgg cgcgcacggt aagtcacgcg atgacttcgg
    19501 cgatatgtac gaagggctgt tgcagaaaaa cgccaacgaa accaagtctg gcgcgggcca
    19561 gtacttcacc ccacgtccgc tgattaaaac cattattcat ctgctgaaac cgcagccgcg
    19621 tgaagtggtg caggacccgg cagcaggtac agcgggcttt ttgattgaag ctgaccgcta
    19681 cgttaagtcg cagactaacg atctggacga ccttgatggc gacacgcagg atttccagat
    19741 ccaccgcgcg tttatcggcc tcgaactggt acccggcacc cgtcgtctgg cgctaatgaa
    19801 ctgcctgctg cacgatatcg aaggcaacct cgaccacggt ggcgcaatcc gtctgggcaa
    19861 caccctgggt agcgacggtg aaaacctgcc gaaggcgcat attgtcgcca ctaacccgcc
    19921 gtttggtagc gccgcaggca ccaacattac ccgtaccttt gttcacccga ccagcaacaa
    19981 acaattgtgc tttatgcagc atattatcga aacactgcac cccggcggtc gtgcggcggt
    20041 ggtggtgccg gataacgtgc tgtttgaagg cggcaaaggc acggacatcc gtcgtgacct
    20101 gatggataag tgtcatctgc acactattct gcgtctgccg accggtattt tttacgcgca
    20161 gggcgtgaag acgaacgtgc tgttctttac caaagggacg gtggcgaacc cgcatcagga
    20221 taagaactgt accgatgatg tgtgggtgta cgacctgcgt accaatatgc cgagcttcgg
    20281 caaacgcacg ccgtttaccg acgagcatct gcagccgttt gagcgcgtgt atggcgaaga
    20341 tccgcacggt ttaagcccgc gtagcgaagg ggaatggagt tttaacgccg aagagacgga
    20401 agttgccgac agcgaagaga acaaaaacac cgaccagcac ctggctacca gccgctggcg
    20461 taagttcacc cgcgagtgga tccgcaccac gaaatccgat tcgctggata tctcctggct
    20521 gaaagataaa gatagcattg atgccgacaa cctgccggag ccggatgtat tagcggcaga
    20581 agcgatgggc gagctggtac aggcgctggg cgaactggat gcgctgatac gtgaactggg
    20641 agcgagcgat gaggcggatg cacagcgtca gttgctggaa gaagcgtttg gtggggtgaa
    20701 ggaatgagtt tcaactcaac atcaaaagaa ttaattgagc aaaatatcaa tgggttactt
    20761 tcaatccatg atagctggtt acgtatttca atggattccg tagcaaacat cacaaacggt
    20821 tttgctttta agtcgtcaga atttaataat cgtaaagatg gtgtaccatt aattagaatt
    20881 cgtgatgttc taaaaggtaa tacatcgact tattactcgg gtcaaattcc tgagggatat
    20941 tgggtatatc ccgaagactt aatcgtggga atggatggtg attttaatgc aactatatgg
    21001 tgctcagaac cagctctgtt aaatcaacga gtatgtaaaa ttgaagttca ggaagacaaa
    21061 tacaataaaa ggtttttcta tcacgcctta ccaggatatt tatccgcaat caatgcaaat
    21121 acgtcctcgg ttactgtaaa gcatctttct tccaggacac ttcaagatac cctacttccg
    21181 ttgccaccac ttgccgagca aaaaatcatc gctgaaaaac tcgatacgct gctggcgcag
    21241 gtagacagca ccaaagcacg ccttgagcaa atcccacaaa tcctgaaacg ttttcgtcag
    21301 gcggtgttgg ctgcggcagt aactggtagg ttgaccaaag aagataa
//
LOCUS       DAPHJE010000078        21337 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPHJE010000078 DAPHJE010000000
VERSION     DAPHJE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.078"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(57..965)
                     /gene="melR"
                     /locus_tag="RH222_002950"
     CDS             complement(57..965)
                     /gene="melR"
                     /locus_tag="RH222_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV3797939.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSSFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1248..2603
                     /gene="melA"
                     /locus_tag="RH222_002951"
     CDS             1248..2603
                     /gene="melA"
                     /locus_tag="RH222_002951"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV3797940.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2706..4127
                     /gene="melB"
                     /locus_tag="RH222_002952"
     CDS             2706..4127
                     /gene="melB"
                     /locus_tag="RH222_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV3797941.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4266..4895)
                     /locus_tag="RH222_002953"
     CDS             complement(4266..4895)
                     /locus_tag="RH222_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV3797942.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5018..6664)
                     /gene="fumB"
                     /locus_tag="RH222_002954"
     CDS             complement(5018..6664)
                     /gene="fumB"
                     /locus_tag="RH222_002954"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV3797943.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6742..8082)
                     /gene="dcuB"
                     /locus_tag="RH222_002955"
     CDS             complement(6742..8082)
                     /gene="dcuB"
                     /locus_tag="RH222_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV3797944.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8653..9372)
                     /gene="dcuR"
                     /locus_tag="RH222_002956"
     CDS             complement(8653..9372)
                     /gene="dcuR"
                     /locus_tag="RH222_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV3797945.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9369..11000)
                     /gene="dcuS"
                     /locus_tag="RH222_002957"
     CDS             complement(9369..11000)
                     /gene="dcuS"
                     /locus_tag="RH222_002957"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV3797946.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11181..11411
                     /locus_tag="RH222_002958"
     CDS             11181..11411
                     /locus_tag="RH222_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV3797947.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            11423..11695
                     /locus_tag="RH222_002959"
     CDS             11423..11695
                     /locus_tag="RH222_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3797948.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11922..12218
                     /gene="ghoS"
                     /locus_tag="RH222_002960"
     CDS             11922..12218
                     /gene="ghoS"
                     /locus_tag="RH222_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV3797949.1"
                     /translation="MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12246..12419
                     /gene="ghoT"
                     /locus_tag="RH222_002961"
     CDS             12246..12419
                     /gene="ghoT"
                     /locus_tag="RH222_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV3797950.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12538..14055)
                     /gene="lysS"
                     /locus_tag="RH222_002962"
     CDS             complement(12538..14055)
                     /gene="lysS"
                     /locus_tag="RH222_002962"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV3797951.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QAQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14292..15749)
                     /gene="dtpC"
                     /locus_tag="RH222_002963"
     CDS             complement(14292..15749)
                     /gene="dtpC"
                     /locus_tag="RH222_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV3797952.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15808..17955)
                     /gene="cadA"
                     /locus_tag="RH222_002964"
     CDS             complement(15808..17955)
                     /gene="cadA"
                     /locus_tag="RH222_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV3797953.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18035..19369)
                     /gene="cadB"
                     /locus_tag="RH222_002965"
     CDS             complement(18035..19369)
                     /gene="cadB"
                     /locus_tag="RH222_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV3797954.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19735..21273)
                     /gene="cadC"
                     /locus_tag="RH222_002966"
     CDS             complement(19735..21273)
                     /gene="cadC"
                     /locus_tag="RH222_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV3797955.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
BASE COUNT         5542 a         5040 c         5219 g         5536 t
ORIGIN      
        1 ttcactgctt attatcgacg gctaaactat ttttttggct gatactgata tcgtctttag
       61 ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg cgacatgccg
      121 acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc cgtcagggca
      181 atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt gatgcgcatc
      241 gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc cattgcatag
      301 ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg atcatagttt
      361 tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga gacgctgttt
      421 ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agccagagag gctaaatcgc
      481 ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcgtt cggactgttt
      541 aattcctgct gccagcggcg cacttcaaac gagctaagtt gctgtgtggc cagtgatttg
      601 atcaccatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca ggagagaaac
      661 agatgcatcg gcagattaaa aatcgccatg ctctgacagg ttccggtatc tgttagttgg
      721 tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac tttttcattg
      781 ttgatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg accatgccag
      841 tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg gtattccgaa
      901 tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa cgtatctgta
      961 ttcatggatg gctctctttc ctggaatatc agaattatgg caggagtgag ggaggatgac
     1021 tgcgagtggg agcacggttt tcaccctctt cccagagagg cgaggggagc gtccgagcat
     1081 catgaggccg aaaactctgc ttttcaggta acttattacc ataaactcag atttactgct
     1141 gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag gttttctgca
     1201 gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg atgactgcac
     1261 ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt cttggtgatg
     1321 tgttccatcg cgaggcgctg aaaacggcgc atattgccct gatggacatt gatcccaccc
     1381 gcctggaaga gtcgcatatt gtggtgcgta agcttatgga ttcagcaggg gccagcggca
     1441 aaatcacctg ccacacccaa cagaaagaag ccttacagga tgccgatttt gtcgtggtgg
     1501 catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc tgtaagcggc
     1561 atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg cgcgcgctac
     1621 gtaccattcc gcatctgtgg caaatttgcg aggacatgac ggaagtctgc cccgatgcca
     1681 ccatgctcaa ctatgttaac ccaatggcga tgaatacctg ggcgatgtat gcccgctatc
     1741 cgcatatcaa acaggtcggg ctgtgccatt cggtgcaggg aacggcggaa gagctggcgc
     1801 gtgacctcaa tatcgaccca gctacgctgc gttaccgttg cgcaggtatc aaccatatgg
     1861 cgttttacct ggagctggag cgcaaaaccg ccgacggcag ttatgtgaat ctctacccgg
     1921 aactgctggc ggcttatgaa gcagggcagg caccgaagcc gaatattcat ggcaatactc
     1981 gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc gtcacggaat
     2041 cgtcagaaca ttttgctgag tacacaccgt ggtttattaa gccaggtcgt gaggatttga
     2101 ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag cagctggcga
     2161 actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt aaaccgtcac
     2221 gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagccgagt gtgatttacg
     2281 gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc gtggaagtag
     2341 cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta ccttcgcatc
     2401 tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa gctattctta
     2461 cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact gctgccgtgc
     2521 tgggcattga tgaaatatat gctcttgttg acgacctgat tgccgcccac ggcgactggc
     2581 tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg ggatttattc
     2641 cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca gcgacccgat
     2701 accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg ttcgggaagg
     2761 attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat gtcgttgggc
     2821 tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat gctattaacg
     2881 atccgattat gggatggatt gtaaatgcta cgcgatcgcg atggggtaag ttcaaaccct
     2941 ggatcctgat cggtacgttg gcaaactctg taatcttatt tctcctcttt agtgcgcatc
     3001 tgtttgaagg tactactcag attgtctttg tttgcgtgac ctacatcctc tggggcatga
     3061 cttacaccat tatggatatt cccttctggt cgctggttcc aaccatcacg ctcgataaac
     3121 gtgagcgcga acaactggtt ccttatccgc gtttttttgc cagtctggca ggctttgtta
     3181 cggcaggtgt gacgctacca tttgttaatt atgtcggcgg tggcgatcgg ggatttggct
     3241 ttcagatgtt cactctggta ctaatcgcct tttttattgt ttcaaccatc atcactctgc
     3301 gcaatgtgca tgaagtcttt tcgtcagaca atcaaccgtc tgctgaagga agccatctga
     3361 cacttaaagc catcgttgcg ctaatttata aaaacgatca gctttcatgc ctcttgggta
     3421 tggctcttgc ttataatgta gccagcaaca ttattaccgg atttgctatc tattatttct
     3481 catatgttat cggtgatgcg gatttgttcc cctattatct gtcgtatgcg ggagctgcta
     3541 acctggtgac gttagtattc ttcccacgct tagttaaatc attatcccga cgcattttat
     3601 gggccggagc atctattctt ccggtgttaa gctgtggtgt tctcctgtta atggcattaa
     3661 tgagctatca caacgtcgtc ctcattgtga ttgcgggtat tttgctgaat gtgggaacgg
     3721 cgcttttctg ggtattacag gtcatcatgg tggcagatac cgttgattac ggtgaatata
     3781 aactgcacgt acgctgtgaa agtatcgctt actccgtgca gactatggtg gtgaagggcg
     3841 gttcagcctt tgcggctttt ttcattgcgg ttgtgttagg gatgattggc tatgtaccga
     3901 atgttgaaca gtctacgcaa gccctattag gtatgcagtt tattatgatt gctctaccaa
     3961 ctctgttttt catggtaacg ctgattctct acttccgttt ctatcgcctc aatggcgaca
     4021 cgctgcgcag gatccagatt catctgctgg ataaatatcg caaagtaccg cccgagcctg
     4081 ttcatgctga tattccggtc ggtgcagtga gtgatgtgaa agcctgacgt ggacaatagc
     4141 taacgaaaat ggcctgatgc gatatgttta tcaggccacg tcttaatgta atgctttgga
     4201 tttgcaaatt ttgtagaccg gataaggaat tcacgccgca tccggcatca acaaagcgca
     4261 cgttgttatc cggttatcaa gccaaagcgc cgtagctggc ggcaatgaaa gcgagcgagg
     4321 aatatcaccg tcgttaatgc gccaagcagc gcgcagaaca tatcggattg cgtgtcccac
     4381 tggtcgccct gggtgccgag aaaatcatcc gctccctggc ccattgccag cgccgcccac
     4441 cactcgatta attcatacat cgcgcttatc gccagcgcca cgcagcagac caggaacgcc
     4501 accattttac gtccgcgcac gtacatcccg cgcacgagaa tttctcgtgc caccagtgca
     4561 ggcaccagcc cctggaaaaa atgccccagc ttgtcatacg gattacggct caaccccagc
     4621 cattcctgca cctcaaaacc gatggggact ttcgcgtagg tgtattgccc gccgaccatc
     4681 aggatgatgg cgtgaaagaa aatgagcgta tagagcagcg gcgttaacgg ataacgttta
     4741 gcggtggcaa gcagtagcgg cacgacaata atcaccggtg tcacttccat cagccaggtg
     4801 agtttgtcat gggccgaaat gccggtataa atcaggatta acgttagcgc cagtgcgccg
     4861 gtgttaagaa ttaacggctt gagtgtgcgg gtcatggtga gttcgcaagt cagggaaaat
     4921 actgactatt caccagcaag cgtgaaatta caatcgcaaa agaggtggcc cagggggatc
     4981 acctggcagc atgctgccag gcgctgggcc gaagaggtta cttagtgcag ttcgcgcact
     5041 gtttgttgac gatctgctgg aagaagtcgt tacctttgtc atcgaccagg ataaacgccg
     5101 ggaaatcttc tacttcgatt ttccagatag cttccatacc cagctccgga taagcgacgc
     5161 attccagatg cttgatgctc tgctgcgcca gtaccgccgc cggaccgccg atgctaccga
     5221 ggtagaagcc gccgtgttta tgacacgcgt cggtaacctg ctgactgcgg ttacctttcg
     5281 ccagcatgat catgctgccg ccgtgggatt gcagcagatc cacgtaggag tccatacggc
     5341 ctgcggtggt tgggccaagt gaacctgatg gataaccggc aggggttttc gccggacccg
     5401 cgtagtagat cgggtgatct ttgatgtact gcggaagttc tttaccggcg tcaatcagct
     5461 ctttcagctt ggcgtgtgca atatctcggc ccacgataat ggtgccggtg agcgacaaac
     5521 gagtggatac cgggtattgc gaaagctggg cgaggatctc tttcatcggg cggttaaggt
     5581 caactttcac cgcttcgcct tcaccggcct ggcgcagttc ttgtggaatg tactggcctg
     5641 ggttgtgttc cagtttttcg atccagatac cttcgcggtt gattttcgct ttaatgttac
     5701 ggtcagcgga gcaggagacg cccatgccga ccgggcagga tgcgccgtga cgtggcagac
     5761 ggataacgcg aatgtcgtgc gcgaagtatt taccgccaaa ctgcgcgcca agaccgagtt
     5821 tctgggcctc ttccagcagt tcctgttcca gctggacatc gcggaacgcc tgaccatgtt
     5881 cgttcccttc cgtcggcagt tcatcgtaat agtgagcgct tgctaacttg acggttttca
     5941 ggttggtttc cgcagacgta ccgccaatca caaacgcgat atggtacggc gggcaggctg
     6001 cagtaccgag ggtacgcatt ttctcgacga ggaagttttt cagtttgccg ggagtcagca
     6061 gggctttggt ttcctggtag agatacgttt tgttggcaga gccgccgcct ttcgcaacgc
     6121 aaaggaattt gtactcatcg ccatctaccg cgtacaggtc gatttgcgca ggcaggttag
     6181 tgccggtgtt gacctctttg tacatgtcca gcgccgcatt ctgtgaatag cgcaggttat
     6241 cttcgatata ggtgttatag acgcctttcg acagcgcttc ttcatcaccg ccgccggtcc
     6301 acacgcgctg gcctttttta ccgacgatga tcgcggtgcc ggtatcctgg caggtcggca
     6361 gcacgccttt ggcggcgatt tcggagtttc ttaagaattg cagcgccacg tacttgtcgt
     6421 tttcgctggc ttctggatcg tgaagaatag ccgcaacctg tttctggtgt gccgggcgga
     6481 gcataaaaga agcgtcgtga aaggcttgct gcgccagcag ggtcagggct tctggttcca
     6541 ctttcaggat ggtttcgccg tcgaagtcgg caacgctaac gtaatcggaa gtgagtagat
     6601 agtattcggt attgtctttc cccatcggga aaggtgcctg gtagataaag ggtttgtttg
     6661 acatagcttc cagcctgtaa ctctgtattt gttattcgaa aatggcgtgc cgcgtagtca
     6721 cgggcggcac gcaaagtgca tttataagaa cccgtacatc gcggcgaaga tccagccgaa
     6781 gacgcacgat acgctcacac caatcaaccc cggcagaata aagctgtggt tgatgacgaa
     6841 gcgaccgatg tgggtggtgc cggaacggtc aaactgaatc gctgccagat cgctcggata
     6901 agtcggcagg atgtaataac cgtagcaagc cggtgctgaa gccacgatgt atgccggatc
     6961 aacgccgatc gccagcgcga ccggaacaat cgccgccagc gccgcagcct gagagtttac
     7021 aaacttggaa accagcagca gaacaatggc ataggcccac ggatactctt tcaccatttc
     7081 acccagtacg ccctgaattt cagacatatg cgcaccgaac atggtttctg ccatccatgc
     7141 gataccgtac accgccacga tggcgatcat accggaacgg aagacttcgt tttttgagat
     7201 agacgcggga ttggttttgg tcaggataat aatcagcgcc ccggtcagca gcataaacat
     7261 ctgaataacc agtaccatcg acagcggttt gccgccgaag gatggacgca ggtccgaatc
     7321 agcaccaaga agggcgacta cagcgattgc accgaggaaa atccacattg ccagccagtt
     7381 gcttttcggc agttttttat cgagcagcgt cgcggtatca ccgtaaacat actcacggtt
     7441 ttccggtacg gagatgaatt tctggaactc ttcgtcttta tccagatctt taccgcggaa
     7501 ccagctgaag ataccgatcg ccaggatacc gattaacgtc gatggaatgg tgattgccag
     7561 caggtcgagg aactcaagat ggcgaccatc aaaggtgaca ttacccagca ttgcaaccag
     7621 agacacgacc gcaaccgaca ccggactggc gataatcccc atctgtgcac cgatagaact
     7681 tgccgccatc ggacgttccg gacggatgtt gttcttaatg gcgacgtcgt agatgatcgg
     7741 cagaatggtg taaaccacat gacccgtacc gcaaagaatg gtcagtgtac aggtcacaaa
     7801 cggcgcgaca attgagacat atttcgggtt gcggcgcagc aacttctcgg caatttgcag
     7861 catgacatca agaccgcccg aagcttgcaa ggtcgccgat gccgccacca ccgcaatgat
     7921 aaccagcatg acatcaactg gtggtttacc tggctgaagg tggaagacga agaccagaat
     7981 gaccagaccg ataccgccta ataaacccag cgcgatacca ccctttctgg caccataaaa
     8041 cagacatatc agtattatga taagttggat agtaaataac atgtgtgaac cctcgcgata
     8101 atcctattta aatttttgct gaatagatca cagtcacgtt ctgttttgta tgaactgttt
     8161 tcagagcctg actaaatatc cgtttggtcg ttaccggctt tagcaaatac ctcacagtaa
     8221 atattggctg attgaagcag cggcaaattt atgcactgtg ataagcggct ttttcagaga
     8281 gaaaaactct ctggcgatgg gtttatagtt tgtactgcgc agatgttaat gttttgtttg
     8341 ctgttatctt attgatattt atcggtctat tttttgttct cagggcagat atcattaggt
     8401 ttaaagatta atctgatcta cccatttgtg ggtaaaaata cacataatgc gggtgacata
     8461 atagttaatt aacttttgtt agcgttttga aattaaaaac accgttcacc tgaagagata
     8521 ttaattttta gcgatgatgg agggataatt atatttgatc tggcacaagt tttactgatg
     8581 aaggatgtaa cttgtgccag gggtattttg cattacggta ataattatta cttacagata
     8641 accagcgatc cgttattggc aatattgttt cagtagtgag tagtgttctg cctgaatacg
     8701 gtaacggtaa accggacgcc ccgtgacgcc ataatggata ctggtgaaca agatgtggca
     8761 gttgaccagc cagatgaggt atttacggca ggaaacacgc gagatgttaa cctcgttagc
     8821 cagctcgtcg gttgaaaact catagtcctg atgcgcgtca atccactggc acagcgtgcg
     8881 taacgtctgc ggcgttaagc cttttggcaa gcgacgagga tcctgttcgt tggagctgct
     8941 gccgtggatt agctgatcaa gctcggcctg gtcataatac tgatgttttt ccagcgccat
     9001 tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg gaagcctgga agggtttgat
     9061 caggtaatcc acgacaccgt aatgcagcga atctttaatg gttgccgcat cggctgcgga
     9121 ggagatgaca atcacatcac ttttgcaacg cgcgttatgc aggacaggca gtaaatcgag
     9181 cccgttctct ttttgcatat agatatcgag caatatcagg tcgataggcg tatcgctatt
     9241 gaagataatc tctttggctt tctccagcgt cgaggctgtt ccacagcatt gaaagcctgg
     9301 gatttgtgct acgtatcggc gattcagctc cgcgaccatt gcgtcgtcat cgataattaa
     9361 tacattgatc atctgttcga cctctccccg tcccagggta tctggacaaa aaattgtgtg
     9421 aaaatcccgg gttccgattc cacggcgatg ctgccgccga gattttctac ctgttgtttg
     9481 acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt gtcaaaaatg
     9541 tgatcgattt tgtcgggtgc gatccccggt ccatcatcgt taacttcgca gtgcagccag
     9601 ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg gttccggccc taatgcttcc
     9661 agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg tcgcgacctg gtcctcactg
     9721 ccgctgtctg gcagctggct ttcactgttt aaaatcagcg tatggcctaa atcggtcgcg
     9781 cgattaatct tgctgattaa aaaaccagcg ataaccggag atttgatctt acccagcaga
     9841 gagccaatct cttcctgata gttattggct gttttgagaa tgtaatcttc caactgctta
     9901 taactcttca gatgcaataa tccgagaatc acatgcaatt tattcataaa ttcgtgggat
     9961 cgttcacgaa gtgcatcagc atagttgacc agaccgtcga gtcgctgcat cagtttacga
    10021 acttcagttt tgtccctgaa ggttgaaatg gcaccgatga taacgccatt actgcgcacc
    10081 ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa tctcttcgtc gcggcgcggc
    10141 gtaccgtcgc gtaacacttc cgagacatct accacctgtg accatgagtg gctgagcgtc
    10201 gacagtttct catcgtcctg cgacttacgg taattcagca attcttgtgc ggcatcgttg
    10261 atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc cttctttgat agactgcaac
    10321 atggcctggc gctgctcaaa cagcgtggag atttcgtagg gttccaggcc gaaaaggatt
    10381 tttttcagta ccttaaccag aatgcaggtg ccaatcagtc cgaccagcat gccaaataat
    10441 accgaccaga taatgctcca gcgactgtca ttgatttgtt gggtcacgcg gcttaactca
    10501 aggccgatcg ccaccacgcc aatttgttta tgattttcat cgtagatggg ggtaaatacg
    10561 cgtaaagcct gcgccagaaa accgcgattg atagcgacat tttctttgcc attcagcgct
    10621 ttaaggatgt catcaccttt aaatggctga ccaatacgct gggcttcagg atgcgagtag
    10681 cgaagacttt gcatatcggt aacgacaata aacagcagat cgttgcgttt gcgcacggct
    10741 tccgcgatgg cctggattcc gctctcctgc ggttttttct gcaagccctg acggatttcc
    10801 ggcgagtcgg cgagggtacg cgccactgcc agtgccttgt tggccagccc gtctcgcgtc
    10861 atatcactga tttgcgagaa gtaaatcaga tgcaccacca atagcaccga gaacagtacc
    10921 gcgctgacca ttaagatcac tgtggtactc aatttcatcg gacgtttgcg taacatgcgg
    10981 tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa tttcttaagc attatctctg
    11041 atgaggcggg taattcaaag ggagtaagaa tgattggcta tataggggaa gagactctgg
    11101 caacggaaac tgccagtgct gtatgaagat tccagggcta tgcttatagc gataatcata
    11161 ctgatgagag agggaaagtc atggatcagg cgctactgga cgagggttat cgctgttata
    11221 ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg tcagcattct ggcaattgcg
    11281 tacgtggcaa cggcaagtta tttaatctca aacgaaagcc gtggatcatg ccggatgaag
    11341 tcgacgtcgt cactgtggtt aaagtgattg atacgtgccc gagcggcgcg ctgaaatacc
    11401 gtcataaata agcgagggta aaatggaaat acgcgaaggc cacaataaat tttacattaa
    11461 tgatgaacag ggcaagcaaa tcgctgaaat tgtctttgtg ccgaccggag agaatttagc
    11521 gattatcgaa cataccgatg tcgatgaaag cctgaaaggg caagggattg gtaaacagct
    11581 ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc cattatgtcc
    11641 atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat gatattcgca gttgatggga
    11701 gagtacagat tcacgatatt tttcatgctc tctgcggtgt gatgcaggag agcatctgaa
    11761 gggtaggggg atgcacaaag aatgggcaga gagcgcgttt ttttgtccca agtcatcccc
    11821 tttactgagc aaaaaaaaga atatctccta tatgagaatc atcaatcggg gttaataagt
    11881 tttgcgtccc cagagtgttc aatattgata ggagtcatat tatggaaggt aaaaacaagt
    11941 tcaatactta tgttgtttct tttaattatc catcatctta ttcctcagtg ttcttaaggt
    12001 taagatcatt gatgtatgat atgaatttct cctctatcgt ggctgatgaa tatgggatac
    12061 cacgacaatt gaatgaaaac tccttcgcaa taacgacatc gttagccgca agtgaaatcg
    12121 aagatttaat caggcttaaa tgcttagact taccggatat tgactttgac ctcaacatta
    12181 tgacagttga tgactatttc cgtcagtttt acaagtagcc aggaaggata agaagaacat
    12241 aaaaaatggc actattctct aaaatattaa ttttttatgt gattggtgtg aacatatcct
    12301 ttgtcattat ctggtttatc tcacatgaga aaacacatat tcgtttactt agtgcattcc
    12361 tggtcggaat aacctggcca atgagtctgc ctgtggcatt acttttttct ctcttttagg
    12421 agctgtagtt gagtggaccc gctggttatc gtgaaacgaa gcgtctctcc tcaaaactgg
    12481 atttgcggaa cgatgaacga agcagtcgcc tgactgcttc gttcattaaa gtgaaattta
    12541 tttctgtggg cgcatcgccg ggaagagaat aacgtcacga atagtatgac tgtttgtaaa
    12601 cagcataatc attcggtcga tgccaatacc cagaccagcg gttggcggca gaccatattc
    12661 cagcgcagtc acgtaatctt cgtcatagaa catggcttcg tcgtcacctg cagctttagc
    12721 gttaacctgt tcctggaaac gttcagcctg atcttctgcg tcgtttaatt cgctaaaacc
    12781 attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt ccgggttaac
    12841 atcattacgg cgcgccagcg gggacacttc tgccggatat tccgtaataa aggttggctg
    12901 aatcagatgt gcttctgcca cttcatcaaa gatctccgtg acaatacgtc ccagccccca
    12961 gcttttctct accgtaattc cgatagattc agctaatgct ttagcagcat caaaattatc
    13021 caggtcggcc atatcggttt ctggacgata ttttttgatt gcttcgcgca tggtgagttt
    13081 ttcaaacggt ttgccgaaat caaacacatg ctcgccataa gtgactttag tggtacccag
    13141 aacctcttgt gccagggtgc ggaacagtga ctctgtcagt tcaatcaaat cgtggtaatc
    13201 cgcatacgcc atgtagagtt ccatcattgt gaactcagga ttatggcgaa cagaaatacc
    13261 ttcattacgg aagttacggt tgatttcgaa tacccgttca aaaccgccta caaccagacg
    13321 tttcagatac agctccggcg cgatacgcag atacatatct aaatccagag cattatgatg
    13381 ggtaataaac gggcgagcag atgccccacc tggaattacc tgcatcatcg gggtttctac
    13441 ttccataaag ccgcgcgcga ccatgaactg gcggatagcg gccagaattt ttgaacggac
    13501 aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc agataacgtt gacgataacg
    13561 gacttcctga gcctgcagac catggaattt atctggtaaa ggacgtagtg cttttgtcag
    13621 caggcgcaac tcagtacagt gaatggaaag ctcaccggtt tgcgtcttaa acagcgtacc
    13681 gcgggcaccg ataatgtcac ccagatccca ttttttaaac tgatcgttat aaacaccttc
    13741 tggcaggcta tctcttgcaa cgtacagttg aatacggcca ccgacatcct gcaacgttac
    13801 aaaggaggct ttccccatga tacgacgggt catcattcgg ccagcaaccg agacttcaat
    13861 gtttaaggat tccagttcct ggttatcctt cgcatcaaac tcttcgtgca gctggtcaga
    13921 ggtatggtcg cggcgaaaat cattgggaaa cgccacacct tgctgacgta gtgccgccag
    13981 tttttcgcgg cgatttctca gttcatcgtt aaaatcaata gcctcattgg ctccccgtgt
    14041 ttcttgttca gacattttgg ttcctctaaa tccagctttc aatttagcgt agataaagag
    14101 acagatcggt acactgtatt atctgcctcg actattaata actcagttta tcttatcgaa
    14161 actaacgctt acctcaagtt gatatcacta ataaataacc atcaaaatgg tttaaatgac
    14221 ttattaatct agatttgcta gtgttataaa ctgaaagtta atttattcat aaccaattgt
    14281 ttttacgaca gttaatcgtt gctctcctgt atcatattcg tcggcgtgct aaacagaaaa
    14341 cgggtagcaa aggccaatac gacgatgatc gcgacacaag ccaacgtcca ctctcccatc
    14401 tgagaaaaga atcgctggta agctgcaatt gccataccgc taatttgcga ctctgtcgtc
    14461 tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag catataaata
    14521 ccggttaata cgccagacat tttcagacgc gtgatttgcg caatcgccac cgggtcaata
    14581 aagagttcgg caaagcccat tagcgccaac ccggatatca tcacgcccat tgacgcttga
    14641 ccgtcagccg ctgcatgtcg ggcatcaaat gccaacaaca taaagccaca agccatcagc
    14701 agtaagccaa aggcaaactt cagccagacg cgcaatgttg agttgccgcg gctttctggc
    14761 gacgccagcc aggccagtac aaccccagcg agcatcaccg caattgcatt caccgactgg
    14821 aatagtgctg taggtacttc aatattgaat gcctgacgat tcacaaagcg atcgataaac
    14881 aagctgatgg tactgccgcc ctgttgtgcc agtacccaga acaatgtccc gacaaacatc
    14941 aacaatacaa tttgccaaag agcacggcgg tgttcgggga atttgaccat catgcgggca
    15001 atgatttgtg cggcaatgag gcaaacgatc gccagcaaat atccggacca gtcgttctcc
    15061 agcagcaggg taaaaaatac tggggctaaa cagagcatca ccactaacca gctccatacc
    15121 ggtaaggcaa atttgacgct agtgagcgct tttttatcca tactacgcgt ggactggaaa
    15181 tgacgatgcc cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc cgcaagggca
    15241 aagccaacat gccatccata ccactgagca gccaggccgc aggcgattgg ggcggcgata
    15301 gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg atgatcgttc
    15361 tcgtcgtaaa gctcgccaag caaacagctg atgtttgatt tgaataaacc gtagccacaa
    15421 ataatgattg ccagcgccag atacaggcta aaggttgaat ttgtatcaat gcccagcacc
    15481 acatggccaa gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt gccgagcagg
    15541 cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa ccagagaagc atatgcgctg
    15601 aacaggctga tggcatggtt atcatcaaaa ccaagctgat gggtgagata gagaatgagt
    15661 aaggcacgca tgccgtaaaa actgaagtac tcccagattt ggatcgccac gatatagtat
    15721 atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa aagggaagtg
    15781 gcaagccact tcccttgtac gagctaatta ttttttgctt tcttctttca ataccttaac
    15841 ggtatagcgg ccatcagcct gacggtatgc accgtgaata tcggtttcaa agcccggata
    15901 gtgagcgccg atttcacaca gcatctgcag gaactccaga accggacggc tttcttcggt
    15961 gatcatttca cccggcatta ccagaggaac tcccggcgga tatggaagga tcatattggc
    16021 gttaatacga cctaccattt cgtcgaggta aacttcttcg gtcataccgt gcagctcttt
    16081 ctggaacgca gcatacggag tcattaccat cgtcggcagc acttcaaatg cgcgatacat
    16141 cagatccggc agattgtggt gaacaatcag tttgtggata ttctgagcca gttcctgaat
    16201 acgcatgttt tcatagaatt caggatcttc acgatacaga gacggcagca tgtttttcac
    16261 acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga gcacgcagca ggctcagtgc
    16321 tttggtctta tcgataccga tgctgaacag gaacagcagg ttatacggac cggttttctc
    16381 aacaacgatg ccatgttcgt cgaggtattt cgccacgatg ctggccggaa taccaaagtc
    16441 gctcatggtg ccgtcttttt ccatccccgg agtcagcagg gtgactttga tcgggtcaag
    16501 atacatgtgc tcgttatcga tgtttttgaa gccgtgccag gtgctgtcag aacgcagcgg
    16561 ccagcattca gtggtatcga tatgatccgg ctgccataca tcaaagaacc agccatcaga
    16621 ttccgttctc agacgtttga tctctttacg gaatttgatc gcacgttcaa tggaaccgtt
    16681 gatcagacgc ttaccagcat tgcctttcat catcgccgct gcggtttcag tggacgccac
    16741 gataccgtag tgcggagaag tggtggtgtg catcatgtag gcttcgttaa aggtttcttc
    16801 gtttacgtca cctttaacgt ggatcatgga agcctgagag aacgccgcca gcagtttgtg
    16861 agtggactgg gtttcgtaaa tcactttccc ttctacacgg ccaccgctca taccgcattt
    16921 accttcgtaa atcggtgaga agttggtgta aggcacccac gcggagtcaa agtggatgga
    16981 tttcacatcc agtgttttct tgataaagtc ggtgttgtac agcagaccat cataggtaga
    17041 gttggtaatt acagcatgta ccggccaggt tgcgtttggt gtttctttca cgcgcttagc
    17101 aatggtagcg tgctggaatt cactctgtgg gataccacca agaataccgt aagcgttacg
    17161 ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc atcaggtggg tcagcgattt
    17221 gtggcagtta cggtcaatca gaatggtgct gcctgctgga gcagagtaca taccaacaat
    17281 tttgttcgca gtggaagtac cgttggtcac catgtagctg cggtctgcgt taaagacgcg
    17341 agcgatatac tgttctgctt ctttgtgtgg accactgtga tccagcagag aacccagttc
    17401 agatactgaa atggaaatat cagatttcat ggtgttcgga ccaaagaaat catagaacag
    17461 gctacctacc gggcttttct ggaatgcagt accgcccatg tgaccaggag tacagaaagt
    17521 atatttacct tcacgaacat atttaaacag tgctttagtc agcggaggca gaatagtgtt
    17581 gatatattcg tcagtggtct gcttgatctt attagcaata tcttcagcag cacccagcgc
    17641 atattcaaag aagctaatct gtaaacgcag gtcattcagg cttacatcga gagtggaata
    17701 cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt cttcgcacag
    17761 ctcgagatta tatttatccc agtcaaaaat aacgccgcac agacgcgcat tgttttcgat
    17821 cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca gacgttcaag
    17881 cgcgcgatga agttcacgga tgggttcttc tttaaaataa acccccatgt gattcaatat
    17941 tgcaataacg ttcatagtca tatctccagg taaaaaaggc ccctcccaac acatgggaca
    18001 aaatgaaagg aggagcctcg gaaaatactt ttaattaatg tgcgttagac gcggtgtggt
    18061 tatccattga gtggctctgg cgctcgtgca ttttgcgagc gtagaacatc aggataatca
    18121 ggctgacgat gaaggtacct gccagctcga aggagcttgc gcccatcagc gcgatgaagc
    18181 agaacacgca acccagtaca gagcagatca ggctgacaaa gttgcggatg ttaacgcctt
    18241 caaaacgaat caggtcaacg caagagtaga agtacggcag catagtcagc agtactgcga
    18301 taccggtcag ttcaccgaac aggtcagatg ctttaccacc ggcagagttc atcagagtga
    18361 tgaggatcat cagggcagtc attttcactg cagccagcag cagacctttt ttcggaatac
    18421 cgttgctgtc gacttcacca taaactttcg ggaagttacc gtcgttagcg gcacgtacac
    18481 ctgcctggcc taccaacatc atccaggagc ccagagaagt caggcacgca aaggcggtga
    18541 atgcagaaac cagcggcgca gcccagttac cgaggatagt tgaagcactg attgcaaacg
    18601 gagcaccgga agccgccatt acagaagacg gatacatacc ggaaagcacc tgagtcgcag
    18661 cgatataaac aatacctgct aaaccagtac ccagcatggt tgccagcgga acggtacgtt
    18721 tcgggttttt aaccatacca gtacttactg ctgcggattc aacacccacg aaggcccaca
    18781 ggcagagcag aatactttta atgatcgcat gaccatcagt ggtatccgca gtattccagt
    18841 tagctgcata agttgccgca tcaaaccaat gccagccaac aatagcagtc atcaccacag
    18901 gaataagaac cagcaccaga ccaatagtgg ttaaacggct tacccaggta ccgccgagca
    18961 tatttacaaa ggtaaatacc cagacgatag caatacaggc gatacccgcc ggaacaggat
    19021 catttaatac tgggaagaag gtggaaagat aagatacagc ggtaatacca atcgccaggt
    19081 taccaatcca gttagcatgg taataaagaa cacctgtctg aaaaccaaat gcaggggaaa
    19141 tttctccggc ataagcaatt gggccacctt gttgcgggtt ttttgttgcc agtcgggcat
    19201 atacatacgc cagcgacatt gcaccaataa tagagataat ccaaccccag atagcaatac
    19261 caccgatact tgctaggttc gcaggtaata atgcaatacc gctccccatc atattaccgg
    19321 caacaacacc ggtacaggca aatagcccga tcttcttggc agaactcatg ctcttctcct
    19381 aatttcattt ttgaatttgg agtccgggtc atgatgtata actatttcct gaccagacca
    19441 aactggcgct aagaatactc acgaaaaaag gattaatcct aaagattagg tgaaataaca
    19501 caaaagtttc tgtaagtgag aacttgaggt tttttattaa cacatcagga tcgcaagttg
    19561 atatcatgaa aagataaaca ttcaatgttt acaatggatt gcgggatatt ctctggttaa
    19621 atttatgtaa taaaaattat gcggcaaata aattgccgca acatattata ccaacagaaa
    19681 catacaaaaa ctcaacaaca aatattcccg agcataaatc aacccgggag ttacttattc
    19741 tgaagcgaga aatttgtcga gataaggtac aacataagga acagaagtct ggaatatacc
    19801 attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaggcgg tgagatatgc
    19861 atcagccgct tcccggttca tccccttcat ttcataaacc ttgccaagca acacataatt
    19921 tagccaggac atttcaagat caatgccagt atttatcgcc tggtaagact catctgtttt
    19981 accttttacc agagcactga ccgcttttat ttgatatata atggataggt tattcaattc
    20041 cggtagtgta acaatgttgt ctatttctgt gttcagtgct gctaattgtt tttcatctaa
    20101 agggtgttga gaatggcgca cgatatcaac taatgctttt tctgctctcg cgtaggtaaa
    20161 ttctggggat gattgaacaa tctcacctaa taattcactg gcacggttca atgatttatc
    20221 atcgccatgc agtaaataat catgtgcctg ataaaaatta gttaataacg caccacgatg
    20281 aggcaaaatt tgctggagcg tctcctgcat tcgttgtggc cacggttggt ttaatgcttt
    20341 tgataaactc tccagtaaat cattttgaat cgccagctga ttaccgttag tgatgacata
    20401 acgtttatcc agcatggttg aaccatctgc attgtctacc aattttatcg acataaagca
    20461 ttgttgagca cggtattggc gctgattaac aaaggcaata gataatgttt taccggaact
    20521 gctcggttca tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg agaaggtgtt
    20581 aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa gagagctgat acggggaact
    20641 ccagctgtta caacttttat ttaccatatt aatgtcaata tcgcgtggat tgagcaaaat
    20701 acgcgatttg ctcataggaa gacgtgtatc aagagttgaa aacgctacca gtgctacaca
    20761 gatacctaac gacaacagga aaaaaaacca tacccaaaag gtagtgaatc gtttgctttt
    20821 aactggggat tgttcaggtg gcgttgcggt gttttgaatg ttaagactgt gggagggaga
    20881 atctgtggca ggaaccgcct ctggtatagg gggaggcgaa gatagcatta tttcctctcc
    20941 ctcttcttcg ctgtaccaga taaccggcac cattaattta tagccgcgct ttggtacagt
    21001 agcgatatag acaggactat cttcatcatt atcttttaat gacttacgta gttctgagat
    21061 actctgcgtc acaacatgat tggtgacaat acttctcttc cagacattat cgataagttc
    21121 atccctgcta agtacttcgc cactgtgttg agcaaagaaa accagaagat cgattaatct
    21181 cggctcaagg gtaagttgac gcccattgcg gctaatttgg tttatggacg gagtaacaag
    21241 ccattcgcca acgcgaacta caggttgttg cataatagaa actcattcga aaagggaatg
    21301 atgcaatgat aattgccaca acctattttt accatct
//
LOCUS       DAPHJE010000079        21253 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPHJE010000079 DAPHJE010000000
VERSION     DAPHJE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.079"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..455
                     /locus_tag="RH222_002967"
     CDS             93..455
                     /locus_tag="RH222_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV3797956.1"
                     /translation="MAEYRFTLPYPPSLNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            452..640
                     /locus_tag="RH222_002968"
     CDS             452..640
                     /locus_tag="RH222_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HDV3797957.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRP"
     gene            637..1260
                     /locus_tag="RH222_002969"
     CDS             637..1260
                     /locus_tag="RH222_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV3797958.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            1694..2017
                     /locus_tag="RH222_002970"
     CDS             1694..2017
                     /locus_tag="RH222_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDV3797959.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2001..2477
                     /locus_tag="RH222_002971"
     CDS             2001..2477
                     /locus_tag="RH222_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDV3797960.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDSYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            2474..2941
                     /locus_tag="RH222_002972"
     CDS             2474..2941
                     /locus_tag="RH222_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV3797961.1"
                     /translation="MSRVAAIISALVICIIVCLSWAVNHYRDNAIAYKAQRDKNVREL
                     KLANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            2929..3081
                     /locus_tag="RH222_002973"
     CDS             2929..3081
                     /locus_tag="RH222_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797962.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLEVEHMA
                     RGKGLR"
     gene            3284..3802
                     /locus_tag="RH222_002974"
     CDS             3284..3802
                     /locus_tag="RH222_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDV3797963.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIERLE
                     QQAQISIPGLPK"
     gene            4155..4397
                     /locus_tag="RH222_002975"
     CDS             4155..4397
                     /locus_tag="RH222_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HDV3797964.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            4433..4921
                     /locus_tag="RH222_002976"
     CDS             4433..4921
                     /locus_tag="RH222_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV3797965.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            4899..6398
                     /locus_tag="RH222_002977"
     CDS             4899..6398
                     /locus_tag="RH222_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDV3797966.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKPWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPSPIRPIARRT"
     gene            6399..8564
                     /locus_tag="RH222_002978"
     CDS             6399..8564
                     /locus_tag="RH222_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDV3797967.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPVVRKLVAEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEKKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVGRSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNSVKEVASIGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDIPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYIGVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYLTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            8578..9489
                     /locus_tag="RH222_002979"
     CDS             8578..9489
                     /locus_tag="RH222_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDV3797968.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     VLKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVSPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYDAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            9489..10784
                     /locus_tag="RH222_002980"
     CDS             9489..10784
                     /locus_tag="RH222_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDV3797969.1"
                     /translation="MALNEGQLVTYALDEIIETVKNLTPMASKVTKYTPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGKATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLSGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     TGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            10829..11071
                     /locus_tag="RH222_002981"
     CDS             10829..11071
                     /locus_tag="RH222_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797970.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEPEQEEKQRKKPGRKPKAAADEPDNEG"
     gene            11049..11549
                     /locus_tag="RH222_002982"
     CDS             11049..11549
                     /locus_tag="RH222_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HDV3797971.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     TKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFQRKEP
                     CDNGSE"
     gene            11549..12967
                     /locus_tag="RH222_002983"
     CDS             11549..12967
                     /locus_tag="RH222_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDV3797972.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSLAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            12967..13815
                     /locus_tag="RH222_002984"
     CDS             12967..13815
                     /locus_tag="RH222_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797973.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGASFTATTI
                     KIIAEQ"
     gene            13815..14282
                     /locus_tag="RH222_002985"
     CDS             13815..14282
                     /locus_tag="RH222_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDV3797974.1"
                     /translation="MEIKLIDNPVKLAEFLNNPENTGNIVDSGDKYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHRVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYMSNGKPVGITLYQLPRSKYEELLNVST"
     gene            14269..14928
                     /locus_tag="RH222_002986"
     CDS             14269..14928
                     /locus_tag="RH222_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDV3797975.1"
                     /translation="MLVLSESFKNKLLPMNGYMKGGSDSGSKAQARATEKGIELQREM
                     WQTNMQNLAPFTPLAQQYVSELQNLSSLQGQGQALNQYYNSQQYKDLAGQARYQSLAA
                     AEATGGLGSTATGNQLAAIAPTLGQNWLSGQMNNYNNLANIGLGALTGQANAGQNYAN
                     NVSQLYQQQAAASAANANKPSGFQSALGGAAAGAAAGTAIMPGWGTAIGAGVGLLGSL
                     F"
     gene            14941..16335
                     /locus_tag="RH222_002987"
     CDS             14941..16335
                     /locus_tag="RH222_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HDV3797976.1"
                     /translation="MATWQQAGNSGALLAGLGGMNSNAPRASDADATLAYIRQNNEME
                     RSGRNNVGLQALQGISSVMDMYKQMDQQKRQQEFQQAYADAYTSGDRDAMRKLASQYP
                     EQFDAVRNGMKFVDEDQRSTVGTLAASARLAASSPEAMMSWLQNNSSELTRAGVDPLD
                     VAKMYQQNPQGFTEFVDHLGMAALGPEQYFQAQDRIVGREIDRGKLAETIRSNQAGEA
                     LTARGQNITMRGQDLSASTARRGQDLAMQRASTRGTAGNDERTVQLSDGRTVTVGGKL
                     HGAGANAFYEGIDNEGNMVRVPASSIAAPATSSASAQNYAMKKDIDAIANADASALDF
                     MTGMTGGAGNPAIGADVRSRLTGKEQRQLYNSAQRIQGRMQNQGVAAARDMGASGINT
                     VAEAKMYFQGMPQVDYSSPEAMQQSIREIQEYTNNYNQQYNVNVGKSQRQQSQPAQVS
                     QPAASSNFSSLWGD"
     gene            16335..18332
                     /locus_tag="RH222_002988"
     CDS             16335..18332
                     /locus_tag="RH222_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV3797977.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPEAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTQGLEQDFGLQKGALTPQTTEGRVF
                     AEALPYLTPAGVERAAVQAPTLAGRIAQGATRLLAENAVGSLAANSMKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPDGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRAQMRNGIIGKAMEKSGGSPDQFLRQLNILQDQTGITFKGQEAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINAMTQGAKSDALSE"
     gene            complement(18423..18908)
                     /locus_tag="RH222_002989"
     CDS             complement(18423..18908)
                     /locus_tag="RH222_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797978.1"
                     /translation="MKKSLLIIPLLLAGCAKISDYQASCEQRYPKLSDMANCLDASVK
                     SDSRMASAPTPKLYVLAAKMLGQGVDDGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            19043..19462
                     /locus_tag="RH222_002990"
     CDS             19043..19462
                     /locus_tag="RH222_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797979.1"
                     /translation="MKRIIGVVAGVIMLSGCATIVGDETQLVQVNSNPSGASFKVKDE
                     SGVIVAQGKTPQGVTLAKSDGSYFGKKSYQITMEKDGYEPVTLPIKANANGWYIGGNL
                     VFGGLIGWLAVDPFNGGMYTLKPKEANASLIPSTKQD"
     gene            complement(19479..19730)
                     /locus_tag="RH222_002991"
     CDS             complement(19479..19730)
                     /locus_tag="RH222_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDV3797980.1"
                     /translation="MGRDDPQFNLRLPYELKEKLKQRAKSNGRSLNSELVQIVTDAVS
                     KPSKISGYRDDAERIADEQSELVKKMVFDTLKDLYKKPT"
     gene            19866..20222
                     /locus_tag="RH222_002992"
                     /pseudo
     CDS             19866..20222
                     /locus_tag="RH222_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129900.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT         5904 a         4999 c         5414 g         4936 t
ORIGIN      
        1 ttggattata cgatgacata tctctagccg ttaatgccag aaaattggcg gaatcacgat
       61 tgggctatca ccataatcat gggagaggat aaatggcaga atacagattc acacttccgt
      121 acccaccgtc gctgaacacc tactggcgaa gacggggaag ccaatattac ataagcgata
      181 aaggccagaa ataccgaaaa gacgttcagc aaatcatcca ccaactcaag ttagacattt
      241 tcaccaaatc acgactccgc atcaaagtaa tcgcagacgt tccagactcc cgccgccgcg
      301 acctcgataa catcctgaaa ggtttactcg actcacttat ccacgccgga tttgcggaag
      361 acgacgagca attcgatgac attcgcgtaa ttcgtggcgt gaaagtacca ggcggaaggc
      421 ttggaataaa aatcaccgaa ctggagaacg catgaacgcc acaattcaaa ctataccaga
      481 gcttcttatc cagacacgag gcaatcagac cgaagtggcg aggatgcttt cctgcgcaag
      541 aggaacagtg ctcaagtaca accgagacag caaaggtgag cgtcacgtaa tagttaacgg
      601 cgtcctgatg gtcaaacagg gcaagagggg aagaccatga gactcgaaag cgtagctaaa
      661 tttcactcgc caaaaagccc gatgatgagc gactcaccac gggccacggc ttctgactct
      721 ctttccggta ctgatgtgat ggctgctatg gggatggcgc aatcacaagc cggattcgga
      781 atggctgcat tctgtggtaa gcacgaactc agccagaacg acaaacaaaa ggctatcaac
      841 tatctgatgc aatttgcaca caaggtatcg gggaaatacc gtggtgtggc aaagcttgaa
      901 ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat tcgcttatgc ggattattgc
      961 cgtagtgccg cgacgccggg ggcaagatgc agagattgcc acgggacagg tcgtgcggtt
     1021 gatatagcca aaacagagca gtgggggaga gttgtcgaga aagagtgcgg aagatgcaaa
     1081 ggcgtcggct attcaaggat gccagcaagc gcagcatatc gcgctgtgac gatgctaatc
     1141 ccaaacctta cccaacccac ctggtcacgc actgttaagc cgctgtatga cgctctggtg
     1201 gtgcaatgcc acaaagaaga gtcaatcgca gacaacattt tgaatgcggt cacacgttag
     1261 cagcatgatt gccacggatg gcaacatatt aacggcatga tattgacttt ttgaataaag
     1321 ttgggtaaat ttgactcaac gatggataaa tgcactcgtt aaataaagcc ctgagttaat
     1381 agctctgggc tttttgcgtt ttaatcacga cctttctgaa agcacatcaa accaaatacc
     1441 agacagacaa aaataatcac cttatccgct gtggctacgg tgtggtgtgc tttgcataaa
     1501 agaaaaccag cgcaatggct ggcttcgtga aagcgggtgg caagaggttg cgctaacaac
     1561 ctcctgccgt tttgcccgtg catatcggtc acgaacaaat ctgattacta aacacagtag
     1621 cctggatttg ttctatcagt aaccgaccat attcctaatt aaatagagca aatcccctta
     1681 ttgggggtaa gacatgaaga tgccagaaaa acatgacctg ttagccgcca ttctcgcggc
     1741 aaaggaacaa ggcatcgggg caatccttgc gtttgcaatg gcgtaccttc gcggcagata
     1801 taatggcggt gcgtttacaa aaacagtaat cgacgcaacg atgtgcgcca ttattgcctg
     1861 gttcattcgt gaccttctcg acttcgccgg actaagtagc aatctcgctt atataacgag
     1921 cgtgttcatc ggctacatcg gcactgactc gattggttcg cttatcaaac gcttcgctgc
     1981 taaaaaagcc ggagtagaag atggtggaaa tcaataatca acgtaaggcg ttcctcgata
     2041 tgctggcgtg gtcagaggga actgataacg gacgtcagaa aaccagaaat catggttatg
     2101 acgtcattgt tggcggagag ctattcactg attactcaga tcaccctcgc aaacttgtca
     2161 cgctaaaccc aaaactcaaa tcaacagcag ccgggcgcta ccagcttctt tcccgttggt
     2221 gggattccta tcgtaagcag cttggcctga aagatttctc tccgaaaagt caggacgctg
     2281 ttgcgctgca gcagattaag gaacgtggcg ctttgccgat gattgatcgc ggtgatattc
     2341 gtcaggctat cgaccgttgc agcaatatct gggcttcact gccgggggct ggttatggtc
     2401 agttcgagca taaggctgac agcctgattg caaaattcaa agaagcaggc ggaacggtca
     2461 gagagattga ggtatgagca gagtagccgc gattatctcc gctctggtta tttgcatcat
     2521 cgtctgtctg tcgtgggctg ttaatcatta ccgtgataac gccatcgcct acaaagccca
     2581 gcgcgacaaa aatgtcagag aactgaagct ggcgaacgcg gcaattactg acatgcagat
     2641 gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag ctgatgcgaa
     2701 agctgaaaat gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg
     2761 aaaatgccct gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc
     2821 caccgtcgaa ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaattat
     2881 ccgcgaccaa acagcactga gaacgcttca ggaatacatt aggacgcaat gccttcgatg
     2941 atagcgataa ttttactcat catccttcac atctggctct gtagacaggg tggtgatcac
     3001 ttctggagta aatccagatt aaacatctca ttgctgatgc ttgaagttga gcatatggcg
     3061 cgcggtaagg ggctgcgttg agataagagc cagttcatta caaataccag gatttagcct
     3121 cgcattcgcg gggcttttta tatctgaatt tcacagcgca tctcacgcgc atattaacga
     3181 gagcctttca gtaagcgagc ctgagaaatg ccgttatagg tggcgacctc tctcgggcgg
     3241 cttttctgtg agacaggctc actttctaaa aggtaaagac gctatgaatc atcaattggc
     3301 taatctcgat ttccgggaca tggtggttgt ttctggtgat cgcgtgatca caacctcccg
     3361 caaggtagca gcttacttcg acaagcagca tcaccacatc attcagaaaa tcgaaaagct
     3421 agactgttcg gatgaatttc taaccagcaa cttttcgcgg gttacctatg aacacaaggg
     3481 taatcagtat gttgaatatg aaatttccaa agacggtgcg atgtacatca tcatgtcgtt
     3541 taccggcaaa aaagctgccg ccatcaaaga ggcgtttatc aaagcattta attggatgcg
     3601 tgacaggctg atggagatgg ctcactcata ccaaagagag cacaacgagt taatgctgga
     3661 gttcatgaag gaaaaggatg ttgccagtat gtcaggacgc ttgctgaacc gctggggcag
     3721 gatcaaaaaa ccgcaactca tagcaagaat cgaaaggctt gagcagcagg cgcaaatatc
     3781 gatccccgga ctgccaaagt gaccattcca aagcccatct acgggtgggc ttgataatga
     3841 aaccgtgatt tacatcccca caatccgggt atgtaaaaga tagttcaggc gagaacagat
     3901 ttaactaaat ctgtgcacca ccagttacgg cagtaccacg aagcaaccca agccagtaag
     3961 tggggaaata acactggcag ccactgaaag atgaacctcc tgccttatgg caaaaaagat
     4021 tctttgtggt ggcggactga tggaaagaca tcggttattg cagagatcat tcaatgagtg
     4081 gtctcgacaa tggcttatac cctacacggg atgacttaac tgatatccct tttaacggat
     4141 aaacggagcc aacaatggca gagattattc ccatgactga agaacagaaa ttccagttag
     4201 agatttacaa actggtcatg aaccagaacg cagccgcaga agaagcattt cagttcattg
     4261 gcactgacga gctgaagctt gagctattca aaattcactt ccagtcaggt ggcgctaatt
     4321 cagatatcac gaccagaact atcgaagcgg tgcgtaaatc gaaggaagcg ttagacctgt
     4381 tcactaccgg agcatgacgc tcaacctgaa ataacaacta agtgagatga atatggcagc
     4441 accaaagggc aaccgatttt gggaggcccg cagtagtcat gggcgaaatc ctaaattcga
     4501 atcgcccgag gcgctgtggg ctgcttgttg tgaatacttc gagtgggctg atgataaccc
     4561 gctatgggag ggtaaggtat tttcatatca gggagaaata attaaggcta atgtccctaa
     4621 gatgcgagcc atgactattt caggattgtg taccttcctt gatatcacca ggcaaacatg
     4681 gggaaccttc cggtcaatgg aaggtttttc tgacgtcaca tcacgagcgg aagacatcat
     4741 ctacgaccag aaattctctg gagcagccgc tgaccttctc aacgctaaca tcatcgcccg
     4801 tgatttgggc ctcaaagagc agtcgcaagt tgaagacgtg acacctgata agggagatcg
     4861 cgataagcgg cgctctcgta tcaaggagct attcaaccgt ggaactggac gcgattcttg
     4921 ataacctgag cgacgaagag caaatcgaat tgctcgagct actcgaagaa gaagagaact
     4981 acaggaacac acacctgcta tatgaattta cgccatacag caaacagcgt gagttcatcg
     5041 acgccgggca tgactatcca gagcgctgtt ttatggctgg taaccagctt ggtaagtcat
     5101 ttactggtgc tgctgaagtc gcgtttcacc ttacagggcg ttatccggga acaaaaggct
     5161 atccggctga tggtaaatat ggcggggagt ggaaaggtaa gcgtttctat gagcctgttg
     5221 tcttctggat tggcggcgag acaaacgaga ctgtaaccaa aacgactcaa cgcatcctgt
     5281 gcggtcgtat cgaagagaat gacgagccag gctacggttc cataccgaaa gaggacatca
     5341 ttagctggaa gaagtctcct ttctttccga accttgttga tcatcttctg gttaagcatc
     5401 acacggctga tggcgttgaa gatggcattt caatctgcta cttcaaacca tactcgcaag
     5461 gacgtgctcg ctggcagggt gacacaatcc acggcgtgtg gtttgacgaa gagccaccat
     5521 acagcattta tggcgaaggg cttacccgta ccaacaaata cgggcaattc tcaattctga
     5581 cgtttacccc gctgatgggg atgtctgacg ttgttaccaa gttcctgaag aatcccagca
     5641 agtcgcagaa agtggtcaac atgaccatct atgacgctga gcactacacc gacgagcaga
     5701 aagagcaaat catcgcatcc tatcctgagc atgagagaga ggcgcgtgct cgcggtattc
     5761 ctacgatggg tagtggtcga atcttccaga taccggaaga gacgattaag tgtcagccgt
     5821 tcgagtgtcc tgatcacttc tacgtaattg gcgggatgga tttcggatgg gatcaccctc
     5881 aggcgcaggt tcagctttgg tgggataagg acgcagacac aatctacgtt tcgcgcgtgt
     5941 ggaaggcgaa agaaaagaca gctgttcagg cgtggggagc cgttaaacca tgggcgcata
     6001 aagtgccaac cgcatggccc catgacggaa accagcacga gaagggcggc ggtgagcagc
     6061 ttaaagggca gtacgcggac gctggtttta tgatgttgca ggagcatgcg acatggcctg
     6121 atggcggtaa cgctgtggag cctggaatca ctgaattgcg cgacatgatg cttgatggtc
     6181 gcttcaaagt attcaacacc tgtgagccat tctttgagga gttccgcctc tatcaccgtg
     6241 atgaaaacgg gaagatcgtc aagcttaacg acgacgtgct atccgccgtt cgctatgcat
     6301 acatgatgcg ccgcttcgca aaaatgatgc gcgacatcaa aaaaccaaaa gagaaaaaga
     6361 taccatcccc aatcaggccc atcgcacgga gaacttaaat ggccgacgaa aacagactca
     6421 attccattct gtgtaagttt gacgcagact ggatggcgag cgatgaagcc agaaccgagg
     6481 cgacaaatga cctgtatttt agccgagtgt cgcaatggga tgactggcta tcaaactaca
     6541 ccaccctgca atatcgcgga caattcgatg ttgtccgccc ggttgtcagg aagttggtcg
     6601 cagagatgcg ccggaaccct atcgacgttc tcttccgacc aaaagacggt gctaatcctg
     6661 atgctgccga tgtgctgatg gggatgtatc gtactgatat gcgccataac acggcaaaaa
     6721 ttgccgttaa tgttggcgtt cgtgagcaga tagagtcagg cgttggtgca tggcgtctgg
     6781 tcacgcagta cgaagacaac gacccaacaa gcaacaatca ggtaatcaga cgcctgccaa
     6841 tccatgaagc ctgctcacac gtcatatggg acgccaacag caagcagatg gataagagcg
     6901 acgctaagca ctgcacggtg attaacgcct tgtcacgcaa tggctggaaa gagttcgcag
     6961 aggattacgg tattgatcct gacaccttgc catctttcca gaatccgaac gatacatggc
     7021 tgtttccgtg ggtatcgaat gatgtcgtct acgtcgctga gtattacgag gtcgaagaga
     7081 agaaggagaa ggtcttcatc taccgcgacc cgctgacagg tgagccggtc agctattacc
     7141 agcaggatat caaagacgtc atcgacgacc tggctaatcg tggattcatt aaggtagcag
     7201 agcgtaaggt caagcgtcgg cgtgtgtata agtcgatcat cacctgcacg cagatactga
     7261 aagaccgcga gaagatagcc ggagagcata tcccaatcgt tcctgtgtac ggcgaatggt
     7321 cattcgctgg tgacaaggag tgctacgaag gagttgtaag gctgacgaaa gacggtcaac
     7381 gccttcgtaa catgatcatg tcattcaacg ccgatattgt tggtcgttca ccgaagaaga
     7441 aaccgacatt cttccctgag caaatcgaag gctacgaata catgtacggt ggaaatgatg
     7501 actatccgta ctatctccag aacaggactg atgaaaacgg taacgacctg ccgattggtc
     7561 caatctccta catggaaaac cctgaagtgc cgcaagccaa cgcttacatg cttgaggctg
     7621 ccaccaactc agtgaaagag gtggctagta ttggcgtgga tgcgcaggcg gcaaacggtc
     7681 aggtagcttt cgataccgtc aatcaactga acatgcgggc agaccttgag acatacgtgt
     7741 ttcaggataa cctggctacc gcaatgcggc gtgatggcga gatttatgcc tcaatggtca
     7801 atgatattta tgacatccct cgtcatgtaa cgctgacact tgaagatgga agcgagaaag
     7861 acgttcaact ctacgcgcaa gttgtcgatt accagtccgg taatgtggtc acactcaacg
     7921 acattcgcgg tcgctacgag tgctatatag gagttggacc atccttccag agcatgaagg
     7981 aacagaaccg cgcagagatt caggagttac tcaccaaggt tccgcaaggt actccagagt
     8041 tccagatgct gatgctgcaa tacctcacgc tgcttgacgg taaaggcgtc gagatgatgc
     8101 gagagtacgc taacaagcaa ctggtgatga tggggctgaa gaaaccagaa acacctgaag
     8161 agatggagat ggtacagcag gctcaacagc agccgcagca gccatcagca gagcaaattc
     8221 aggcgcaggg tatccttctg caaggtcagg ctgaattgct caaggcagag aaccaacagg
     8281 cgcagattca ggttgaagcc gccaaggttg aagcccaaaa ccaactcaac gccgcgaaga
     8341 ttgcagaaat cttcaacaat atggacctcg acaagcaggc agaactgcgt gagtacctca
     8401 agctcgtagg tcaattccag caacagcgca gcaaagatgc tcgtgctaac gctgagctgc
     8461 ttcttaaaga tgcagaccag actcattcac aacgcatgga tttcgcgaat cttatgcgtc
     8521 aagttcaaat cccctccggc ggagtagccg agacacctca ataagagaga gttaatcatg
     8581 gaccaaacca ccgacattca ggcttctgaa gaattaaccc tgcccggcaa tcatgcagcg
     8641 gcatctgctg atggcttagt tgtcgataat gccaacgaca acgcaggtca ggaagaaggc
     8701 ttcgagattg tcctgaaaga cgatgagaaa ccaaaacaag acccggcaac taatgctgaa
     8761 tttgcccgtc gccgcatcga acgcaaacgc cagcgtgagc ttgagcagca gatggaagcg
     8821 gttaagcgtg gagagttgcc ggagcacctg cgggtgagtc ctgagttacc aaaacaacca
     8881 gaccctaacg attatctttc cgaagacgca ctggctaagt acgactatga ccagagccgc
     8941 gcactggctg ccttccagca ggcaaacagt gaatggcaga tcaaggctat ggacgcacga
     9001 agccaggctg tcgccgagca ggggcgaaaa actcaggagt tcacccagca atcagcgcaa
     9061 tacgtcgagg cagcccgtaa gcactacgac gcagcggaaa agctcaacat ccctgactat
     9121 caggagaaag aggatgcatt catgcaactg gtgccgccag cagtcggtgc cgatatcatg
     9181 cgtctcttcc cggagaaatc cgctgctctc atgtatcacc ttggtgctaa tcctgagaaa
     9241 acacgccagt tgctggcgat ggacgggcaa tccgcgctga ttgaactcac tcgactgtca
     9301 gaacgtttaa ctctcaagcc tcgagccaag cctgtttcag aagccccgtt acctgatgaa
     9361 cccattcagg gacacgctgt tgctgcaaat atctctgcga ttgaaaagca gatggaagcg
     9421 gcagcaaaca aaggggatgt agagacatac cgcaagctca aggcgcaact gaataaagga
     9481 attcgataat ggcattaaat gaaggtcaac tggtcacgta tgctctggat gaaatcatcg
     9541 aaaccgtcaa gaacctgacg ccaatggcgt ccaaagtgac aaaatacacc cctccggcag
     9601 aatccatgca gcgttcaagc aacaccgtgt ggatgcctgt tgagcaggaa gcgccaaccc
     9661 agactggctg ggatttaact ggcaaagcaa ccgggattct ggaactctcc gtgaaatgca
     9721 acatgggcga tccggataac gatttcttcg agcttcgtgc agatgacctg cgtgatgagc
     9781 gttcttaccg tcgccgcatc caggcatccg ccaaaaaatt ggcgaataac attgagtcag
     9841 caattgccaa acaggcaacc gaaatgggct cacttgttgt tcacgatacc cgcgcaattg
     9901 gtccatctac tggcctgtct ggctgggatt ttgtgtctga tgcagagcga ctgatgttct
     9961 cccgcgaact caaccgcgac atgggaatca gttacttcct gaaccctgac gattaccgca
    10021 aagcaggccg caacctggta gatggtgaca tctttgggcg cgttcctgaa gaagcgtatc
    10081 gtaacggtac tattcagcgt cagattgctg gctttgatga aattcttcgc tcaccgaaac
    10141 ttccggcagt taccaagtca accgctactg gtgtaactgt ttctggtgcg cagaagttta
    10201 agccgcaggc atacaccctt gataccgatg gtaacaaaga gaacgtcgac aaccgtgttg
    10261 caacggtgac cgtatcctca accaccggat ttaagcgcgg cgacaaaatc agcttcactg
    10321 gtgtgaaatt cctgtctcag atggcgaaga acgtgctgac tgatgatgct actttctcaa
    10381 tcacccgtgt gatcgatggt actcacatag aaatcacgcc gaagccgatt gcgcttgatg
    10441 acgcttcact gacaaaagaa gagaaggctt acgctaacgt aaacacctct cttgctgata
    10501 caactccggt aaacgttctg aacgtggcaa caaccaccgc taacgtgttc tgggctgatg
    10561 actcaatccg tctgctgtct cagccgatcc cggtaaccca tgaactgttt gctggcatga
    10621 aaacgtcttc cttcagcatt cctggtattg gtgttaacgg catcttcgca acgcagggag
    10681 atatcaacac cctgtctggt aagtgccgta ttgctgtgtg gtattcagca tgtgctgtac
    10741 gaccagaggc aattggtgtt ggtctgccta accagactgc gtgataacca gagggagctt
    10801 cggctccctt ttttatctgg agacaagcat gacacacatg atctttcgtc atggcgacat
    10861 gaagaagtgg aaaggcgttg gctacgactt tgaaatcgtg aaagccgaag agcttcagga
    10921 atatctggat gctggctggt tttcacatcc tgatgacctt ttgaaggatg ttgcagagcc
    10981 agagccagag ccagagcaag aagaaaaaca gcgtaaaaag cctggtcgaa aacctaaggc
    11041 ggcagcagat gaacctgaca acgaagggtg atttagttct tgcggcatta cgtaagctcg
    11101 gtgtggcatc aaatgccacg ttaaccgatg tcgaaccgca gtctatggaa gacggcgtca
    11161 acgaccttga aatgatgatg gctgaatggc ttggcggtga tgcgtcacca ggtatcaacg
    11221 ttggctacat tttcgctgat gcagatgtcg ctccggatcc tggcgatgag cacggtttgt
    11281 caaataacgc tatcaatgcc gtcattttca accttgcctg ccgcattgct ccagattatg
    11341 cgctggaagc ctctacaaaa cttataacca ctgccagata cgggaaagag cgactagtca
    11401 aactgtctgc aatggacaga gcaaaagccg ctaaatgtaa gtccggttat ccaaaccgta
    11461 tgcctgttgg tagcggtaat cagttggcga agtggaacgg ttggaattac ttccagcgaa
    11521 aggaaccttg cgataacggg agcgaataat gccgattcag caacttccgc tcatgaaagg
    11581 tgtcggcaaa gacttccgaa atgccgacta tatcgactat ctgccagtga atatgctggc
    11641 aacccccaaa gaaatcctga atagcagcgg atatcttcgc tcattcccgg gcattgccaa
    11701 acgttctgat gtgaacggcg tatcgcgagg cgtcgagtac aacatggcac agaatgctgt
    11761 ttatcgcgtg tgtggtggca aactgtataa gggcgaaagt gaggtcggtg atgttgccgg
    11821 aagtggtcgt gtatcattgg ctcatggtcg gacatcacag gcggtaggcg ttaatggtca
    11881 actggtcgag tatcgctatg atggcacggt taaaaccgtc tcaaactggc ctacagacag
    11941 cggattcacg cagtatgagt taggctcagt ccgcgatatt acgcgcttgc gtgggcgtta
    12001 tgcgtggtca aaagacggaa ctgattcatg gtttatcact gaccttgaag acgaatcgca
    12061 tcctgaccga tacagcgcac aatatcgtgc cgagtctcag cctgacggca tcatcggaat
    12121 cggaacatgg cgagacttca tcgtctgctt tggttcatcg acgattgaat atttttccct
    12181 gacaggtgca accaccgttg gtgccgcttt gtatgtcgca cagccatcac tgatggtgca
    12241 gaaaggcatt gccggaactt actgcaaaac gccgtttgct gattcgtatg cgtttatcag
    12301 caatccggca acgggtgcgc catctgtata catcatcggc tccggtcagg tgtcaccaat
    12361 cgccagcgcg agcattgaga aaatactacg ctcctacact gctgatgaac tggctgatgg
    12421 cgtgatggaa tcgttgcggt ttgatgctca tgagttgctg attatccacc ttcctcgcca
    12481 tgttctcgtg tacgacgcat cttcaagcgc caatggtccg caatggtgtg tgttgaaaac
    12541 tggcttgtat gacgatgtgt accgcgctat tgacttcatt tacgaaggaa atcagataac
    12601 gtgcggcgat aagctggagt ccgtgaccgg gaaattacaa ttcgacatca gcagccagta
    12661 cgacaagcaa caggaacatc ttctgtttac tccgctgttc aaagcggata acgccagagt
    12721 gttcgacctt gaggttgaat cgtcaactgg cgttgcgcag tacgccgacc gcctgttcct
    12781 ctctgcaacc actgacggca tcaattacgg tcgtgagcag atgattgagc agaatgaacc
    12841 gttcgtttac gacaaacgtg ttttgtggaa gcgagtcggg cgaatcagga aaaatgtcgg
    12901 cttcaaattg cgcgttatca cgaagtcacc tgtcactctt tctggctgcc agataaggat
    12961 tgagtaatgg ctgattcgaa tctcaatgtg ccggtaatca ttcaggctac acggctcgac
    13021 acatcagtcc ttccacgcaa tatcttctcg cagtcgtatc tgctttacgt tatcgcacag
    13081 ggcactgatg ttggtaacgt ggctaacaag gccaacgagg ccggacaggg cgcttatgat
    13141 gcacaggtca ggaacgatga gcaggatgtg attctcgctg accatgagca gcgaatttct
    13201 gctgcggaag caacgcttgt taatcatgag gagcgaatca gccaggcaga atcaactctt
    13261 caggaacatg aaacacgaat agctcagaat gaaagcgata ttgcgtcgct tgataccaga
    13321 gttcagtcgc tggaatcgca ggtttcagat catgaaacgc gcatcgatgc tctggagtat
    13381 gccactactc gcaagaagtc agaggttgtt tactctggcg tatctgtaac catcccgaca
    13441 gcgccgacca accttgttag cctgctgaaa acgctcacgc cgtcatccgg cacgttggca
    13501 ccattcttcg acaccgttaa caacaagatg gttgtgttca acgagaacaa aaccttgttc
    13561 ttcaagctgt ctattgtcgg gacgtggccc agcggaaccg ccaacaggtc aatgcagcta
    13621 acattttccg gttctgttcc tgacacgttg gttagcagtc gtaatgcggc gacaacaacc
    13681 gacaacatcc tgttagctac gttcttcagc gtggataaag acggctttct tgccacaaat
    13741 ggcagtacgt taaccattca gtcgaatggt gcgtcgttta ctgccacaac catcaaaatc
    13801 attgcggagc agtgatggaa ataaagctca tcgataatcc ggtgaagctt gcagaattcc
    13861 tcaacaaccc ggaaaacacg ggaaatatcg tagacagtgg agacaaatac tacatcaagc
    13921 ctgatgcggt atatctcggc atctacgaag gattagtgct ggctggcgtt catgaagttc
    13981 gtaacttctg gcatagagtt gtggaatgcc atgcggtgta cgaccccgga ttccgtggtg
    14041 aatatgcact gcaagggcat cgattattct gcaaatggct tctcgaaaac tcaccattcc
    14101 ttaacagcat taccatggtt cctgatacca cgaaatacgg acgggcaatt atccgtttgc
    14161 ttggcgctac ccgtgttggt caccttgatg atgcgtacat gagtaacgga aaaccggttg
    14221 gaatcaccct ctatcaatta cctcgttcga aatatgagga gctattaaat gttagtactt
    14281 agcgaaagct tcaagaataa attgcttccc atgaatgggt atatgaaagg cggcagtgac
    14341 tccggctcta aagcccaggc acgcgcaact gaaaagggta tcgaattgca gcgtgaaatg
    14401 tggcaaacga acatgcaaaa ccttgcaccg ttcacgccac tcgctcagca gtatgtatca
    14461 gagttgcaga atctttcctc tcttcagggg caaggtcagg cacttaatca gtattacaat
    14521 tctcagcagt ataaagacct tgcaggtcag gctcgttacc agagccttgc tgctgcggag
    14581 gcaacgggtg gattaggctc tacagcaaca ggaaaccagt tagcagcaat cgcacctacg
    14641 ctcggtcaaa actggctgtc aggtcagatg aacaactaca acaatctggc aaatatcggc
    14701 cttggtgctc ttacaggtca ggcaaatgcc ggacagaact acgctaacaa cgtcagccaa
    14761 ttgtatcaac agcaggcggc agcatctgcg gctaatgcga ataaaccatc aggatttcag
    14821 agcgccttgg gtggagcggc agcaggtgca gctgcaggta ctgcaatcat gcctggttgg
    14881 ggtacagcaa ttggtgctgg cgtcggtctt cttggttcac ttttttaatg gaggtgtctc
    14941 ttggctacat ggcaacaggc tggtaattca ggcgcgcttc ttgccgggtt aggcggcatg
    15001 aactccaacg ctccaagagc aagtgatgca gacgccacgc ttgcatacat tcgacagaat
    15061 aacgagatgg agcgttcagg acgtaataac gttggcttgc aggctttgca aggcatttca
    15121 tctgtcatgg atatgtataa gcagatggat cagcagaagc gacagcaaga gtttcagcag
    15181 gcttatgctg atgcatatac atctggtgac cgcgatgcaa tgcgaaaact ggcatcacag
    15241 tatcctgagc agtttgacgc tgtaagaaac ggcatgaaat ttgtcgatga agaccagcgt
    15301 tccactgtcg gtacactggc agcaagtgcc agactcgcag cttcatctcc agaagccatg
    15361 atgtcatggt tgcagaacaa ctcatctgag cttactcgtg ccggagtaga ccctctggat
    15421 gtggcgaaaa tgtatcagca aaatccacaa ggtttcacag agtttgttga tcaccttgga
    15481 atggctgcac ttggtccaga acaatatttt caggcacaag acagaatcgt tgggcgtgag
    15541 attgatcgcg gaaaacttgc agagacaatc cgcagcaatc aggctggcga ggcgctaaca
    15601 gcacgaggtc agaacatcac gatgcgcggt caggatttat ctgcttctac tgcgcgacgc
    15661 gggcaggatt tagcaatgca gcgagcgtca acaagaggaa ccgctgggaa tgatgagcgt
    15721 acagttcagt tatcagatgg cagaactgta acggttggcg ggaaacttca cggcgctggg
    15781 gcaaatgcgt tctacgaagg catcgacaac gaggggaata tggttcgcgt tcctgccagt
    15841 tcaatcgcag cgcctgcgac atcgtctgca tcagcacaaa actatgccat gaagaaggat
    15901 atcgacgcga tcgcaaatgc agacgcttct gctctcgatt tcatgacagg aatgaccggc
    15961 ggcgcaggta acccggcaat tggtgctgat gttcgcagcc gattaacagg aaaagagcag
    16021 cgccagttat ataactcagc acaacgtatt cagggcagaa tgcagaatca gggtgtggcg
    16081 gcagcaaggg acatgggggc cagtggtatt aacaccgttg cagaagcgaa gatgtatttt
    16141 caggggatgc cgcaggttga ctattcaagc ccggaggcta tgcagcagtc gattcgtgag
    16201 attcaggaat acaccaacaa ttacaaccaa caatataacg ttaatgttgg taaatctcag
    16261 cggcagcaat ctcaacctgc acaggtatca cagccagcag ccagcagtaa cttttcttca
    16321 ctatggggtg attaatggct aaagcatgga aagatgttat cgcctctcca cagtatcagg
    16381 cgttagcacc agaacaaaaa gcgcaggctc aggagcaata cttcaatgaa gtcgtggccc
    16441 cgcaagccgg agaaaatgca gagcaggcta agcaagcttt ctatgctgcc tatccattgc
    16501 catctgtgca gccagtggag acacaacaac cagaagcaca gcaacaacca cagcaaagtg
    16561 gatttatgtc tgatcttggt gaagcagtaa aagagactgg tcgcggactg gtgcaggctg
    16621 gcgtgaacgt ggcaaacata cctgcatcag ttgcagatgc tgtaacaagc gcggcggctt
    16681 gggctggcgg taaactcggt attggcgatg gtacatatca accagcacca cgagtaacaa
    16741 cgcagggatt agagcaggac tttggccttc agaaaggtgc gctgactcca caaacgacag
    16801 agggaagggt atttgctgaa gcattgcctt acctcactcc tgctggcgtt gagagagcgg
    16861 cggtacaagc accaacactt gctggtcgaa ttgctcaggg ggcaactcgc cttctcgcag
    16921 aaaacgcagt tggatcactt gctgcaaaca gtatgaaaga tgatgcggaa gcactcgcta
    16981 ccgatttagg tgttggcgtt ctggcaggcg gcgctattaa cgctgctgga cgtggattag
    17041 gtgctgctta tcgtggcgtt cgcggtgcta ttgcgccaga agcgcagcaa gctatcagat
    17101 ttgcagagcg tgaaggagtt cctctgcaca ccacagacct gttacaaccc acttcccgag
    17161 tcggaaaaat ggcgcagact acagcagaaa atatccctct ggctggcaca agcggaatga
    17221 gagcaacgca acaggaagcg agaagccagt tggtgcagag atttgctgat aaattcggtg
    17281 agtatgatcc agcggttgtt attgacagcc ttaaagcgaa aacatcagga attcgtcgtg
    17341 ccgccggtaa tcgactggag caggttcaga atgctatggc tggcgtaaac attcagcctg
    17401 cgcgagcaat tcagcagatt gatactgaga tatctaatct gcagaagctt ggtaaggtag
    17461 ctgataacga gacaatttca aaacttcagt catatcgtga tgagcttgtt cgcaatgctg
    17521 gccctgatgg tccggtaaat ctggatttga agcaattaag cgacctgcgc agtcagttca
    17581 gaatggacgt gaagggtgag cgaccagtgt taccaaaccg ttccgatgct gccattcagc
    17641 gcgtttacaa ggcgatgacc gacgatatca atggtgccat tggtcagaat cttggcaacg
    17701 atactctccg taaatatcag caggccaatg ccgtctacgc tgacgaagcg gcgaaactaa
    17761 agaataccag gctgaagaat gttctcatga aaggcgacct gacgccggaa gttgtcaaca
    17821 acatgctatt cagcaagaac aaatcggaaa ttaagacgct gtataactca gttggtcgtg
    17881 ttggcagggc gcaaatgcgc aatggcatca ttggaaaggc gatggagaaa tcaggtggtt
    17941 cccctgatca gttccttcga cagcttaaca tcctgcaaga ccagactggc atcacattta
    18001 aggggcagga agccgcttat ctgaaaggat tgaaaaacta cctgcaatcc acgcagcagg
    18061 ctgcaaaagc ggcagtaaca acaccaacag ggcagcaaac catcccgttc attatcggat
    18121 atgggacagc aatgaaccct gcgacaactg gcgcagcggt aagctacgga cttcttactc
    18181 gcgcctatga gagcgagcca ttcagaaatg caatgctacg aatggcaaac acaccacgcg
    18241 gatcaacagc gtttgagaaa gccatgcagc aggcacaaaa ggcaattaac gctatgacgc
    18301 agggggctaa gtctgatgca ttgtcagaat agcttttcaa acaccaggaa cgtgcaaaaa
    18361 ccaaatatgt agaacgcgag gtttatcgta tccctctgca taggcgatac ctttgctgat
    18421 tgttatctga tgttactgct actgttgcat gtgactgtat ttccaaaccc tgaattgcag
    18481 tttgtgtaag tgtcaactcg tgttggatag ggttgagtta taacaggctg tctcgctttt
    18541 tgctcgatcg cttgcattgt gtttacagcc tgataattca ataaagcctg ctggaatgct
    18601 tggctttgtg ctatttgttg ggcttgttct tggctttgta attgaacata aagattctga
    18661 agctcaagtc ttgcctgcgc gtcacttatc ttgccatcat cgacaccttg cccgagcatc
    18721 ttcgcagcaa ggacatataa cttaggtgtt ggtgctgatg ccatgcgtga gtcgctcttc
    18781 acgctagcat caaggcaatt agccatatcg ctaagctttg gatagcgttg ttcgcaactt
    18841 gcctgatagt cgctaatctt tgcacaccct gccagcagaa gcgggataat taacagtgat
    18901 tttttcatat gattaactct ccttatcttt gccatcatgg cactgttggg tgtaaatgag
    18961 ttattaactc aatcgacaat atcttcacga aaatactttt tattattaag atctttctga
    19021 ttctaacaaa acggaaagta atatgaagag gattatcggc gtcgttgctg gcgttatcat
    19081 gttatctggg tgcgcaacta ttgttggtga cgaaacacag cttgtgcaag tgaacagcaa
    19141 tccttccggt gcgagcttta aggtaaaaga cgaatcaggc gtgattgttg cgcaaggtaa
    19201 gaccccgcaa ggagtaactc ttgccaagtc agatggtagt tattttggca aaaagagcta
    19261 ccagatcact atggagaagg atgggtacga accagttacc ctgccaataa aagccaatgc
    19321 taatggttgg tatattggtg gaaaccttgt gtttggtggg ttaattggtt ggcttgctgt
    19381 agatccattt aatggtggga tgtatacctt gaagcctaaa gaggcaaatg catcccttat
    19441 accgtccaca aagcaagact aataaatgga acccaccatc aggtgggttt tttgtacaaa
    19501 tccttcagcg tatcaaacac catcttctta acaagttcag actgctcatc agcgatgcgt
    19561 tccgcatcgt ctcgatagcc tgaaattttg gatggctttg atacagcatc agtcactatc
    19621 tgaactaatt ctgaattaag agagcggcca ttggatttgg ctcgctgttt tagtttttcc
    19681 tttaattcgt aaggtagccg cagattaaat tgcgggtcat ctcttcccat tcttgatgcc
    19741 tcacttttgt aagtggatcg gcatcatatg atctactggt tatatccaca ataagaccac
    19801 tgtggtctca atgacgcatt gccgtagcca cgctgcggcg tttccttcta tctggagcaa
    19861 attaaatgac agacattaca gccaatgttg tagtaagcat gccttcgcaa ctcttcacta
    19921 tggcgcgttc ttttaaagcg gttgccaatg gtaaaattta tatcggtaaa attgacactg
    19981 acccggtaaa tcctgaaaac cagattcagg tttatgtgga gaatgaggat ggttctcacg
    20041 ttcctgtttc gcagccaatc atcattaact ccgctggtta tcctgtatat aacggacaga
    20101 ttgccaaatt cgtgacagtt caaagccata gcatggctgt gtatgatgca tatggagcac
    20161 agcaatttta cttctccaat gtcttaagat attcaccaga tcagctaaga tatgatcttt
    20221 cacagccaga tggcgcgaaa ttagtaggat ttaaagacac caatgtatac gatgcaataa
    20281 gctcaataag cgtagatttc tcagcgcttg gagagtcaaa tggagccacc ttggttggct
    20341 atagggacag caccgtttat acagctcttg atagattata cagatcgtta ggaggcgtga
    20401 ttatatctcc tcaatggaaa ccagttgcag gatcatcaac tataagcttt tctggcggtc
    20461 tttattcttt taatggcgtt agtgttattg gtcaaaatca gaatgtcaca acaacatcaa
    20521 gcacaatatg tattgttgtt aaatcagacg gaacatatga agcgcgtgac agatatccat
    20581 ttgacggctc gctacttatt gctcatatta aatcagggac tcttatagaa ttaattggca
    20641 atgcaaataa aagtgctttt ggaattgccg gatgcccatc atcaatacca tggtttccag
    20701 gtgagctgag aagaatagaa gagcagattc caatttactg cgcacttcgt tctgatggca
    20761 cgcttagata ttggactgat tatgatgtgg ctgtgaagaa ggcatccagc gggacaacat
    20821 attatgtgga ctgcactaac ggtagtgata cgactggaga cggcacgcag gataaaccat
    20881 tccagcgtat aaaatatgcc attgagaaaa ccccggcagc aaggacaatc atgataaaag
    20941 gcgggatgga atatacccgt gattttacat ggaatgttag cgtagttgac cgcgagctcg
    21001 atttcatcgg atacgatgga acaccaattc tgagcactgt tgaaccggct gcaacgtggg
    21061 cggcacaggg aagcgctggc gtataccagt acacaggtaa tcttgttcta aacgttgttg
    21121 actatgccaa tcttgatagt tatggacatg ctcaggttct aactcccgta gccagccttg
    21181 ccgactgcgt ttcaacacct ggctcatcct ataaaacagg gaatacgctg tatattcatc
    21241 tttttgacgg gcg
//
LOCUS       DAPHJE010000080        21138 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPHJE010000080 DAPHJE010000000
VERSION     DAPHJE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.080"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            222..722
                     /gene="tssB"
                     /locus_tag="RH222_002993"
     CDS             222..722
                     /gene="tssB"
                     /locus_tag="RH222_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV3797981.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            757..981
                     /locus_tag="RH222_002994"
     CDS             757..981
                     /locus_tag="RH222_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797982.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1032..2507
                     /gene="tssC"
                     /locus_tag="RH222_002995"
     CDS             1032..2507
                     /gene="tssC"
                     /locus_tag="RH222_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV3797983.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2514..2927
                     /gene="tssE"
                     /locus_tag="RH222_002996"
     CDS             2514..2927
                     /gene="tssE"
                     /locus_tag="RH222_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV3797984.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGNQHYRVD"
     gene            2931..4781
                     /gene="tssF"
                     /locus_tag="RH222_002997"
     CDS             2931..4781
                     /gene="tssF"
                     /locus_tag="RH222_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDV3797985.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4745..5827
                     /gene="tssG"
                     /locus_tag="RH222_002998"
     CDS             4745..5827
                     /gene="tssG"
                     /locus_tag="RH222_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDV3797986.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5852..7132
                     /gene="tagH"
                     /locus_tag="RH222_002999"
     CDS             5852..7132
                     /gene="tagH"
                     /locus_tag="RH222_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDV3797987.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7129..7653
                     /gene="tssJ"
                     /locus_tag="RH222_003000"
     CDS             7129..7653
                     /gene="tssJ"
                     /locus_tag="RH222_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDV3797988.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7656..8987
                     /gene="tssK"
                     /locus_tag="RH222_003001"
     CDS             7656..8987
                     /gene="tssK"
                     /locus_tag="RH222_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDV3797989.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRTGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8992..9753
                     /gene="icmH"
                     /locus_tag="RH222_003002"
     CDS             8992..9753
                     /gene="icmH"
                     /locus_tag="RH222_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDV3797990.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9762..12527
                     /gene="tssH"
                     /locus_tag="RH222_003003"
     CDS             9762..12527
                     /gene="tssH"
                     /locus_tag="RH222_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDV3797991.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12524..13267
                     /gene="tagO"
                     /locus_tag="RH222_003004"
     CDS             12524..13267
                     /gene="tagO"
                     /locus_tag="RH222_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDV3797992.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13272..14684
                     /gene="tssA"
                     /locus_tag="RH222_003005"
     CDS             13272..14684
                     /gene="tssA"
                     /locus_tag="RH222_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDV3797993.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14793..18227
                     /gene="tssM"
                     /locus_tag="RH222_003006"
     CDS             14793..18227
                     /gene="tssM"
                     /locus_tag="RH222_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDV3797994.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVQPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18238..19590
                     /locus_tag="RH222_003007"
     CDS             18238..19590
                     /locus_tag="RH222_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDV3797995.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDTLEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            19614..20091
                     /locus_tag="RH222_003008"
                     /pseudo
     CDS             19614..20091
                     /locus_tag="RH222_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
     gene            20135..21049
                     /locus_tag="RH222_003009"
     CDS             20135..21049
                     /locus_tag="RH222_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3797996.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT         5058 a         5694 c         5769 g         4617 t
ORIGIN      
        1 gaattgcaca ggttcataca atctatttaa cgcttgcaga atacccctct ttacgatcga
       61 taaaaagtcc ttttcttatt tattttgaag cgtaaaatgc ggggtgatga aggagtggtg
      121 attttatttt caccgccatc aaggcggcat ttccgatatt ttttgcggta cgaatatgcc
      181 gtgttcatgg cctgtacgag ataacatgac ggatcacttt tatgagcaaa aaatttgaag
      241 gcagcgtcgc gcctcgcgag cgtattaata tatcgtatgt cccaaaaaca gatggccaga
      301 cggctgaagt ggaacttccg cttaacatgc tcgttgtcgg cgataccgga aatacgcagg
      361 aaacatcctc actggatgaa cgtcaggcag tttccgttaa caagcacaat ttcggtgcag
      421 tgatggctga agcagccatt ggcctcaatt ttactgtccc cgccaccctg aagggaagca
      481 caacagacga tgaactgaat gtggcgctga atatcaaatc actggatgat ttttcaccag
      541 atagcgtggc ccggcaggtt ccggaagtga ataaattgct ggaactgcgt gaagcactga
      601 ctgcactgaa ggggccgatg ggtaaccttc cggcattccg tacccagctc caggcattgc
      661 tggaaaatga agagtcccgc gagcagttgc tcaaagaaat cggtctggtg agcaataaat
      721 aaccaccact atttcattaa acagggttat ccaggcatga cggcacacaa aaatatctcc
      781 gggacatacc acctctctgt tgcagacatt ctgcaggtgg tttaccaggt atgtttcagc
      841 ccgtccgttg aaatcaacca ggatggtgtg gctgcgctga ttacaacact ggatcgccgt
      901 atcagtgatc tattggatga gattatccat ttttgtgagt ttcagcagag cgcgtcgcac
      961 tggcagcgcg tacttcactg acggcgaaaa gccggtttca gaaacacagc taaaacagaa
     1021 ggattttcag tatgtcttta caggaagagg aactcgtatc cagtcatgcc gggcagcctg
     1081 agcaggcatc gtcgctgctt gatcaaatca tggcacagac ccgtattcag cccggttcgg
     1141 aaggttatga cgttgcccgc cagggtgtga cagcctttat tgccagcatc ctgcaatcca
     1201 ctgcatctgc cgaaccggtc aataaactgg ccgtggacag catgattgca gatattgatg
     1261 aacgcatcag ccgccagatg gatgtcatca tccatgcgcc tgcctttcag caggtcgagt
     1321 cgttctggcg ctcactgaaa accatggtgg atcgcgttga tttccgtgaa aacatcaagg
     1381 tcaacgtgct tcatgtcacc aagcaggaac ttctggaaga ctttgagttt gcgccagaaa
     1441 ttattcagtc cggattttat aagcacgttt attcgtccgg tttcggacag tttggcggtg
     1501 aacccatcgc tgccgtgctg ggcgcttatg agttcaaaaa taccgcgcct gacatgaaac
     1561 tgctgcaata cgtcagtgcc gtgggcgcaa tggcgcacgc accgttcctg tcctccgttt
     1621 ccccggagtt tatggggctg aactcgtgga ccgaactgcc taatatcaaa gatctttatg
     1681 ccatctttga aggtccggcc tacaccaaat ggcgcgccct gcgtgactcg gaagattcgc
     1741 gctatttagg gcttacggca ccccgcttcc tgcttcgcca gccctattca ccaacagata
     1801 accctgttaa gaactttaac tattacgaag atgtcagcca gaaccacgaa gattatctgt
     1861 ggggtaatac ggcctggatg ctggcatgca atatcgcaga cagttttgcc aaataccgct
     1921 ggtgtccaaa cattatcggt ccgcaaagcg gcggcgcagt gaaagatctg ccggtgcatc
     1981 tgttcgaaac gatggggcag attcaggcca aaatcccaac cgaggtgctg gtcaccgatc
     2041 gccgcgaatt tgaactggct gaagagggtt ttatcaccct taccatgcgt aaagactctg
     2101 ataatgcagc ctttttctct gcaaactcgg tacaaaaacc gaagcacttt ccgggaaaag
     2161 atgcagaaac caactataag ctgggtacgc agcttcctta tctcttcatc atcaatcggt
     2221 tagcgcacta catcaaggtg ttgcagcgtg aacaactggg gtcatggaaa gaaaggagcg
     2281 acttagaaag agagcttaat acctggatcc gtcagtatgt tgccgaccag gaaaacccgc
     2341 ctgcagacgt gcgcagccgc aaacctctgc gcgctgccaa agttgaggtt atggatgtag
     2401 aaggcgaacc cggctggtac caggttgcgc taagcgtgag acctcatttt aaatttatgg
     2461 gggcaaattt tgagctttcc ctggttggcc ggttagacag ggagtaacac accatgatac
     2521 cggatcggga aagaaccaca ggttctcttt ttgagcgtat ggaagcctct tcggccagaa
     2581 accggcaggg aggcagtatt cattcgctca ggcaatccat tcgccagaac ctacgaaaca
     2641 ttcttaatac ccggtccggt agttgccggg gtgcgcctga gctgggcatt gatgaaccgg
     2701 aaggggctga aaacttccgc gaatccatga gtcgggccat cgagcaatgt atagaacgct
     2761 atgaaccccg gatctcacac gctgaagtgc aggcagtggt ttcatccgcc tccagcccgc
     2821 tggatatgac gtttcatatt acagcctggg tgacatttaa cgaaacgcat gaggtgcttg
     2881 aatttgatat ggcaccgaat ggcaaccagc attaccgggt ggactgacaa atggaatttg
     2941 aagaacgcta tttccgggaa gaactcgatt acctgcgcca gcttagcaag ctgctggcaa
     3001 cggaaaaacc ccatctggcc cgcttcctgg ccgaaaaaga tgcggatccg gatattgaac
     3061 gcctgctgga aggggtggct tttcttaccg gcaatctccg ccagaaaatt gaggatgaat
     3121 tcccagaact gacgcacggg cttattaaga tgctatggcc taattacctg cgtccggttc
     3181 cggcaatgac ccttattgaa tatacgccgg atatggataa gtcttctgta ccggtgttaa
     3241 tcccccgtaa tgagcagttt acaaccaacg ccggggaaat cagagttgat gaagtgctgc
     3301 cctctgatgc taaaaaggag gagccgcctc cctgtacctt caccctctgc cgggatatct
     3361 ggctgctgcc cgttcgcctg gagcagattg aaaaccgcag tacgacccgt aatggtgtta
     3421 tcaacatcac cttttcggtc gcaccgggaa cggacttccg cacgctggat ctgaacaaac
     3481 ttcgcttctg gctcggcaat gacgacaact atacccgtga ccagctttat ttatggttct
     3541 gcgaatactt gcagggtgcc gacctgactg tgggtgaaca gcatattcgc ctgcctgagt
     3601 ttatgctaaa agctgtcggt tttgagccgc aggatgccat gctgccctgg ccgaaaaacg
     3661 tccacagcgg ctaccggatc cttcaggagt atttctgtta ccccgatgcg tttctctttt
     3721 ttgatctttg tggttgtccg gctttgcctg acggattgca ggcggaattc tttaccctgc
     3781 aactgcgttt ttcgcgccct ttgcccgtgg acatccggct gcgccgcgat tccctgcgcc
     3841 tgtattgcgc acctgccatt aatttattta tccaccatgc agaagccatc acgctggaca
     3901 accggcgggc agactatccg ctggttccca gccgccatta cccacaacat tacgatgtat
     3961 tttccgttaa cagtgtggtg agccaggtcc aggatatgtt caggaaaaaa gatctggggc
     4021 gtcctgtttc gacgcaggcc gcgcgccagt ggccagcctt tgaaagtttc agccatcaga
     4081 tggaatacag ccggaagcgg gaagtggtgt actggcatca ccggaccaaa acatccctgt
     4141 tccatcgcgg ctttgatcat acccttgcct ttatacatgc tgatggcagt tatccgtcag
     4201 acgaatctct gctcagtaat gaagtggttt cggtatcgct gacctgtacc aaccgtgagc
     4261 ttccgtcaca aattcgttcc ggcgatatca ccggcacaac cggtaaaaat gcggctgttg
     4321 cttcatttcg caacattacc cgcccgacgc aaccactctg gccggtcatt gatggcagcc
     4381 tgcactggtc cctactctcc gccatgaacc tgaattatct gtcattactg gatacggacg
     4441 cgctgaagca ggtcatcgcc aactttgatc gccacgcaat ccatcatccg cagacggcac
     4501 ggctgtcaca acaaaagctg gatgccattg agcgtctgga gacccgcccc gttgatcgcc
     4561 tgtttacggg tattcccgtc cggggactgg cctccacgct gtatctgcac ccggagccgt
     4621 ttgtctgtga aggggaaatg tatctgctcg gtacggtgct ttcgcatttt ctgtcgctgt
     4681 acgccagcgt taactcattc cacatgctga ccgttgtgaa cacagaaagc caggagacat
     4741 ggaaatggac ggaaagaatc gggcagcatc ctcttatctg agtccgggaa atcccccggc
     4801 agataaagag cagaatgatc cgctggcaca ggttttccat aatgcctgct cttacaattt
     4861 ttttgcgatg gcggagctgt tgcaccgcct ggcaaagggt gaaaagggga cgccagaatt
     4921 atccctgcgt gacgatccgg cacaggaaac cctgcgtttc agcgccgatg ccagccttgc
     4981 cttcccgtgc agtgatatca gtgcgctgaa acgggatacg tcaggggcat tcaggatgac
     5041 caccaccttt atggggttac aggggagcca gtcgcctctc cccggttatt acctcgatca
     5101 cctggcctgg aaagcggtac atgaacaaag tccggtaggt gattttctgg atatgttcag
     5161 ccaccgcctg acccagtttg tctggcatat ctggcgtaag taccgctatc acatcagttt
     5221 tcgtaacggc ggtgtggatg cgttctcgca gcgcatgtac tcccttgtcg ggctgggcca
     5281 ccgccagctt cgcgataagc tggcgattaa tcacagcaaa atgctggcgt attccgggat
     5341 cctggcaaat ccgggacgat ccccggaaat aatctgtggc ctggtgtccc actgctttga
     5401 tttgtcagaa gtgacgctgc aaaactggca acgtcgcaag gtggatattg aaccggatca
     5461 gcaaaatagt ctggggagct attctcttaa aaatggcgaa aaactggcgg gaagatcggt
     5521 actgggcaac tttgttctgg gtacacgagt gcccgatctc agcggaaaat tccagctcag
     5581 tattaccagc ctgacccgaa aacagttcct ctcttttctg ccgtccggcg aaaacttcct
     5641 gccactgacg atgttcgtgt ccttcatcct gcgcgatcag cttgcctggg acttacatct
     5701 ggggctggcg ccggagcagg tgggcgcaat gcgcctgggc gataacaaaa gtgcgctgct
     5761 gggctggacc agcttcctcg gcacaccgga agaacgaccc tcagtcacca tcagggtacg
     5821 gtcgtaaatt atgataatca cggagcagaa tatgccggaa gagaaactac agacgctttc
     5881 attgcaggtc attaacggca gtgagctgga aagcggacgg gcggcgcgct gtctgttcac
     5941 acagcaggga aatgtcggcc atggccccga atgccactgg tcggtacagg atcgtcagca
     6001 gagcattccg gcccaggctt ttaccgttat cctgcacgat ggcacatttt gtctgcgccc
     6061 gcagacggca caactgtggc tgaatcaggc aaaagtcaca gcaacatcag acctaataca
     6121 gttgcgccag ggcgatgaga tccagatcgg acggctgatg gtgagggttc atctgaaccg
     6181 gggagatatt ccccattacg atgaggaaat ggccactccc gaaaccatcg ttaccaatcg
     6241 cgatatgctc acggataccc tgctatcaac ggagggtgcg ccacactatc cgggaatgac
     6301 tcaccggcac cagcttgcag acaccgtggt aaacggtttt tctgccgatc cactccaggc
     6361 acttcagtcc gaaagcctga ttaccacggg cgatccgctt tcaggcattg cggctgtccg
     6421 gccatcggca ccgctgtccg atccggcaag taatgggggg atcaatactc cgtttatgga
     6481 tctgccgccc atttatgcca gccccggcga tcgtaatgac gacgtctctg cggcagaaat
     6541 ggcgcaacgc caccttgcgg tcaccccctt acttcgcggt cttggcggct cgcttaccat
     6601 gagcaattcc gacgatgcgg atgattttct ggaggaggcc ggacgaacgt tacaggccgc
     6661 aataaaaggt ctgctcgatt tgcagcagca gcgtaacagc ctctcagaca aacatttgcg
     6721 cccgctggaa gataacccgc tgcgcctgaa catggattac gccaccgcgc tcgacgtaat
     6781 gtttgccgaa ggtaaaagcc cggtacatct ggcggctccc gccgccgtca gtgaaagcct
     6841 gcgcaatgtc cgccaccacg aagaagctaa ccgggcagca attgtggagt cgcttcgtgt
     6901 cctgctggat gctttctcac cacaaaatct gctgcgccgc tttgtgcagt accgccgcag
     6961 ccatgaactg cgccagccgc tggatgatgc cggagcatgg caaatgtaca gccattatta
     7021 cgaagaactg gcctccgatc gccagcaggg gtttgagatg ctgtttaacg aggtctacgc
     7081 ccaggtctat gaccgggtgc ttcgtgaaaa acagcgggag ccggaagcat gaacaggaag
     7141 gcgtttctgg cctgcgtgct gatgtgcgtc atattaaccg gctgcgaaac ggcgaaaaaa
     7201 atcagcgagg tgatcaaaaa cccggatatc caagtcggga gcctgaagga gcaaccttcg
     7261 gaaataaccg tcaccctgct gaccgaaccg gacaccaata ccaatgcgga gggtgaatca
     7321 gccgccgtgg atgtgcaact ggtgtacctg actgacgact caaaattgct ggcagccgac
     7381 tatgaccaga tagccagtac gccgctaccg gatgtgttgg gtaaaaacta tatcgaccac
     7441 caggatttca acctgttgcc ggacaccatc aaaaccctgc cgccggtaaa gctggatgag
     7501 aaaacgcagt ttattggtgt cgtggcctat ttctccgatg accaggccac tgaatggaaa
     7561 caaatcgaga cggtggaagg aaccggccac cattaccgcc tgctggtgca tgtacgtcaa
     7621 agcagcatcg aaatgaaaaa agaggatgaa taattatggc aaccacccgc aacaaggtga
     7681 tgtggcagga agggatgctg atgcgcccac accatttcca gcaacagcag cgttacaacg
     7741 actacctgga taaccagcgt ttccgggcca tgaatgattt atcctgggga tttaccgaac
     7801 tcaccctcaa caatgaactg ctggcgcagg gtaagatcat gattgacagc gcgtcaggca
     7861 cactgcccga cggcaccgtc ttttctatcc ccgaccagga cgcactgccc gatccgctgc
     7921 acccgcaaaa ttttccggac gaaagaagcc gcaatatcta cctcgctctg ccggtcgcca
     7981 gtgatgtgag aaatgaaatc agcgacggac ggcgaaccgg gcgttaccgg ctgaattatg
     8041 ccgatgtccg ggatttgcat tcagaagaag gtgacacgcg aacattgacg ctgggtcaac
     8101 tgacgccgcg cattatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt
     8161 gccgtatcag cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct
     8221 cctgccagaa tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg
     8281 ccattggcgg acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca
     8341 tcgcggatgt ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta
     8401 cccatcgcgc ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgctgggt
     8461 tgctgggtga actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt
     8521 atgatcatca tgatctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc
     8581 tgcataccgt actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggtatct
     8641 ggcaggccga tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg
     8701 cggcaaacgt gccggtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct
     8761 cgccggaaaa aatccgcaat atggtcagtg tgcagatccc cggtattcca ttgcgtgccc
     8821 ttatggtggc tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca
     8881 agagtggcca ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtttccg
     8941 gcagtttccc ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag
     9001 gggtcactgt ctctgccacc atttaccgga cacgacgaaa aaagccagcg caactaccat
     9061 ctcgcgcttc gtggcaacag cctgaatccg atgatcgacg cggccacgcc gctactcggt
     9121 atggtaatgc gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag
     9181 gtggtgacag atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc
     9241 gtgattatct cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac
     9301 ggctggtcaa acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa
     9361 gcctggggtg gcgagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt
     9421 taccaggatc tgctggaatt tctctggatc tgtttttctc tgggatttcg cgggcgctac
     9481 aaagtggcag cacaggatca gggggaattt gagcagatct accgccgtct gtaccacgtt
     9541 ctgcataaac tgcgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag
     9601 ggcgggcgtt atcagctcat cagtcgcctg actgttaaac atattttttg tggtggcgtt
     9661 gtcgtgctgg cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac
     9721 atcctgcacc agttaaacaa attacttcga taaggacgtt tatgatccag attgatcttc
     9781 ccacgctggt aaaacggctg aacctgttct cccgccaggc gctggagatg gccgcctctg
     9841 aatgtatgag tcagcaggca gcggaaatta ccgtcagcca tgtgctcatt cagatgctcg
     9901 ccatgccacg cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt
     9961 tgcgccaggc gctgacggta gagaactaca caaccgcccg ttctgcggac agctacccgg
    10021 cgttttcccc gatgctggtt gagtggctta aagagggctg gctgctggcg tcggctgaga
    10081 tgcagcacag cgaactgcgc ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc
    10141 gttatatacc gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac
    10201 aggactttgt gcagtggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta
    10261 aaggcgcagg cacaatgacg gacgcctctg acaccctgct tgcccgctat gccaaaaaca
    10321 tgaccgcaga cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg
    10381 acttgatgat cgacattctc tgccgccgcc gtaaaaacaa cccggtggtg gtgggcgaag
    10441 cgggcgtggg caaaagcgca ctgattgaag ggctggcgct gcgcatcgtg gcaggccagg
    10501 tgccggacaa gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg
    10561 gggcgtcggt gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggcg gaggtcattt
    10621 cctccccggt gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca
    10681 accagcaggg cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc
    10741 tgaaaaccat cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg
    10801 ccctgtcgcg ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca
    10861 ttattctgcg cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg
    10921 acgccttgca ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg
    10981 acaaagcgat tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc
    11041 cgccgaagca aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc
    11101 agcttgagcg cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc
    11161 tggtgcagta tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc
    11221 agcagacgct ggtccgggag attattgcgc tgcgccagca gttactgggc gtggcagagg
    11281 acgatgcggc gccgttgccg gacgcagata ccgtggagga tacgcagcca gagtcagagt
    11341 cagaacagga taataccggt gccgtaccgg ctgatgaggc cgacagagaa caaccggaag
    11401 agaccgctga aacagtttcc ccggtacagc ggctggccca gctcactgcc gaactggacg
    11461 ccctgcataa cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg
    11521 tgattgccga atggaccggc gtgccgctca accgcctgtc gcagaatgaa atgtcggtca
    11581 tcaccgacct gacgaaatgg ctgggtgaca ccatcaaagg ccaggacctg gcgattgcca
    11641 gcctgcataa gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg
    11701 gtgcgttcct gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg
    11761 cagagctgct ctacggcggt cgccagtacc tgaccaccat caatatgtcc gaattccagg
    11821 agaaacacac cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc tacggtgaag
    11881 gcggcgtact gaccgaagcg attcgccaga aaccctactc ggtagtactg ctcgatgaag
    11941 tggaaaaagc gcacccggat gtgctcaacc tgttctacca ggcgttcgat aagggcgaaa
    12001 tggcagacgg tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca
    12061 acctcggcta ccaggtaata gtcgagcatg ccgatgaccc ggaaaccatg caggaagtac
    12121 tgtatccggt gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc
    12181 cgtatctgcc gctgtcgaaa gagacgctcg ccaccattat cgccggaaaa ctggcccgtc
    12241 tggataacgt gctgcgcagt cgctttggtg cagaagtggt cattgaaccg gaagtgacgg
    12301 acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca
    12361 tcgatggcga catgctaccg ccgctctccc tgctgctgtt gcagaaaatg gcggctaaca
    12421 cggcgattgc ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag
    12481 atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt
    12541 tttccggcag acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc
    12601 gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc
    12661 ggatgcgaca gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga
    12721 gcggcttgac tgctatgacc gcctgctggc accgctgtcc ccgtcagggt ttgacggggc
    12781 gctggtcaaa gccggttttg tgggcgaagc ctggacccgc gccacggaac aggaaaaaca
    12841 ccgccagggc aacaccacag agctgctggt gacgcaggtg ccgggggaac gcccgacggt
    12901 ggtgataacc accccggcta tcggtcatgt tccgccacgc ccggtgctga tgttcagttg
    12961 cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat
    13021 cgccgtcact ctgaatgccg acaaccgggc attgcgcagc cactggtttg tgcgcgagaa
    13081 cggcaccctg ctggagtcca gccgggggtt atccgggatt gatgaaatta agcaactgtt
    13141 cggcgcaaaa acgctgacgg tggataccgg cgcagacaat gccgccggaa agctgacctt
    13201 taacattgac gggctggcaa gggcgattgc tcccctgcgc gatgcctgcc actgggcggg
    13261 agaataaaaa tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg
    13321 aaaacctgcc ggaagagaaa ctggcatcgg cgctgaaaga tgacaacccg aactgggagt
    13381 atatcgacgg cgaaatcgtc aagctgggat cgctggccca cagtcaactg gatatcccgg
    13441 aactccagcg ccgggggctg gtcattctgg cttcggagag taaggatttt cgtctgctgg
    13501 cgcatctgct gagaaccctg caacacgccg gtgatcctca tctcgcattg cgcctgctgg
    13561 cgctgtatgt ggagcattac tgggcggtgg ctgcgccgca gaatatggcg cataaaaaac
    13621 gctttgccag ccaggtcatt aaacgctttg aaacaggtat cgaaggtttt tcacagaacg
    13681 ccgccacagc gcagcgcgat gcgctgtcag gggaactggc aaaactggcg caatgctggc
    13741 agtcacataa cgccccggaa ctggcacagg ctactgatga tctttttgcc ctgtaccagc
    13801 gtgcgtttaa tcgtgcggca ccggctcctg tccccactcc ggcggcctcc ggtagttcac
    13861 cacagaccac cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa
    13921 tcgtcatcga cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa gtggcggcca
    13981 ttttatgtga acgccagccg gattcaccgc agggctaccg tctgcgccgc catgcgcttt
    14041 ggcagaacat caccagtacg ccccaggcgg aaagcgatgg ccgtactcca ctggctgcgg
    14101 tctccgccga tatggtggca gattaccatg ctcagctcgg tagtgccgat atggcgctgt
    14161 ggcagcaggt ggagaaaagc gtactgctgg ctccttactg gctggatggt cactgccttt
    14221 ctgcacagac ggcgctgcgt ctgggttaca aacaggtggc agacgccatc cgcgatgagg
    14281 tcatccgctt ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccacacac
    14341 cgtttatcag tgagcagacg aaacaatggc tggcagcttc gcccgacgcc aaagttgcac
    14401 cggttgcgca aatcggtgag gaatcgaagg cagccagagc ctgttttgct gagcagggtc
    14461 tggaggcggc gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc
    14521 accgccagta ccttgccgca cagttgacgg aagaggcggg gctggtacag cttgcgcagc
    14581 agcagtaccg gatgttgttc aggatgggat tgcagatgat ggtggcagac tgggagccgt
    14641 ccctgcttga gcagctagaa caaaaattca cggcagaaca ataaaagaga acaggagttt
    14701 ttgtgttcag attaccaaca ccccgactac tcagcggact caaatcagcc ctgcgaccgg
    14761 cgatgccccg gtttaaagtc tctgctttct ggctgctgat actggcgtgg atttttctgc
    14821 tggtgtggat ctggtggaaa ggcccgacgt ggacgctgta tgaagaacag tggctcaaac
    14881 cactggcgaa ccgctggctg gcaacggcgg cgtgggggat tattgccctg atgtggctta
    14941 ccgtccgggt gatgaagcgc ctgcaacagc ttgaaaaaat gcaaaagcaa cagcgcgagg
    15001 aagccgttga tccgctcagc gtggaactga acgcccagca gcgttatctt gaccgctggc
    15061 tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagttg ccgtggtata
    15121 tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcgagggg tttccgtccg
    15181 acattattta tgccccggag ggcgcacgcg gcgcagaaca acgcctgtac ctcacgcccc
    15241 atgtcggtaa acaggcggtg atctttgata tcgacggcac actctgcgct cccgctgatg
    15301 cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg
    15361 cgcgccagcc gctgaatggg attattctga cactcgattt acccgatctg cttaccgcag
    15421 acaaacgccg ccgcgagcat ctgttacaga cgctgcgcag ccgcttgcag gatatacgcc
    15481 agcatcttca ctgccagtta ccggtttacg tggtacttac ccggcttgat ttattgcagg
    15541 gttttgccgc cctgtttcag tccctgaaca gacaggatcg cgatgcgatt ctgggcgtca
    15601 cgttcacccg ccgtgcccat gaaaatgatg actggcgaac agagttgaat gctttctggc
    15661 agacatgggt ggatcgaatg aatctggcgt tgccggatct gatggtcgct cagactcaca
    15721 cccgcgcgtc tttattcagt ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt
    15781 cactgcttga gggtctgctt gatggcgaaa atatgaacgt gatgctgcgt ggtgtctatc
    15841 tcacctcttc gcttcagcgt ggacagatgg atgatatatt cacccagtct gccgcccgcc
    15901 agtaccggct gggcaataac ccactggcgt cctggcccct ggtggacacc gcgccttatt
    15961 tcacccgcag cctgttcccg caggcattac tcgcagagcc taatctggca acagagagcc
    16021 gcgcctggct gatacgttcc cgtcgccgcc tgacggtttt ctctgccaca ggcggcgtgg
    16081 cagcactgct gctcatcacc ggctggcatc actattacaa cggtaactat cagtccggca
    16141 tcaccgtgct taagcaggcc aaagccttta tggacgtgca gcctccgcag ggggaagatg
    16201 actttggcaa cctgcaactg ccgctcctga acccggtacg cgatgccaca ctggcctatg
    16261 gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga cgacgtatcg
    16321 ggccttatgt ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt
    16381 ttaacgggct ggtcaaagcg atgaacgccg cgccgccgga gagtgaagaa aaactcgcgg
    16441 tgctgcgcgt gatgcgaatg ctggaggaca aaagcggacg taacaatcag gtggtgaagc
    16501 agtatatggc aaaacgctgg agcgaaaagt tccacggtca gcgcgatatc caggcacaac
    16561 tgatgtccca tcttgactac gcgctggctc atactgactg gcacgcagag cgtcaggcgg
    16621 gcgacggtga cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga
    16681 aagaactgag caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc
    16741 tgggcgttct tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg
    16801 tgtttacatc tgccgatgac aacaaactgg ttgttccaca gtttcttacc cgttacggcc
    16861 tgcaaagcta ttttgtaaaa cagcgcgatg aactggttga actgacggcg atggattcct
    16921 gggtacttaa ccttacccgc agcgtgaaat acagtgacgc cgaccgcgcg gaaatccagc
    16981 gccagttgac cgagcagtat atcagcgact acaccgccac ctggcgggcc gggatggaca
    17041 atctgaatat ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta
    17101 tcagcggcga ccagcctttg cagcgggcgc tgaccgtgct gcgtgacaac acacagccag
    17161 gcgtcttttc tgaaaaactc tctgccaaag aacgggagga agccctggca gagccggatt
    17221 accagttact cacccgcctc gggcatgaat tcgccccgga aaacagtacc ctggcagtac
    17281 agaaagacaa agaaagcacg atgcaggccg tgtatcagca actcaccgag ttgcaccgct
    17341 acctgctggc aatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt
    17401 tacggcttga tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc
    17461 tgcctgctcc gctcaaccgc tgggttggca gactgactga ccaggcctgg catgtggtga
    17521 tggtggaggc tgttcattat atggaagtgg actggcgcga cagcgtggtg aaaccgttta
    17581 acgagcaact ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg
    17641 atgccttcga acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc
    17701 tgaagctgtt tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc
    17761 gcgaagatat tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca
    17821 aacagaacgg tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac
    17881 gccgcagtgt actgaacctt gacggtcagt tagtcgatta cagccagggc cgtaactata
    17941 ccgcccatct ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca
    18001 tcggcaccag cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc
    18061 gcctgttcgg ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta
    18121 gcgtggacgg tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca
    18181 gcggtgggtt gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg
    18241 aatagtaacg tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa
    18301 ttcagcgcca tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac
    18361 tggaaactgg tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac
    18421 accgccacct actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa
    18481 ggtgcggaac tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc
    18541 ggcccggcgc gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt
    18601 cagcaaatct cctttgcgga atccgaccta ccactgatct accgcacaga gcgggcattg
    18661 cagcttatct gcgataagct ccagcaggtg gaactgaagc gcgttccgcg cgtggagaat
    18721 ctgctctatt ttatgcagaa cacgcgtaaa cggcttgaac cccagctgaa gagtaacact
    18781 gagaacgccg cacagaccac ggtcagaacg ctgatttatg ccccggaaac acaggcatct
    18841 tccacaccag aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtg
    18901 cgcagtctga cagagaatcc cccacaggcc agtgtgataa agcaaggcag tacggtaaga
    18961 gggtttatcg cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag
    19021 gtctatccgg tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg
    19081 gtgtggatgg cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat
    19141 acctccccgg tacagccgct ggaaaccggg atgcagatga tgcgtgttgc cgacagtcgc
    19201 tggccggaaa gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc
    19261 cgcgcacaga gtagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct
    19321 tttgcagatc tagtgatgca gcgcgagaaa gagggactaa ccctttccta tatcaaaaat
    19381 gtcatctggc aggcggagcg gggactgggg caggaaacac ccgttgagtc tctgttgacg
    19441 cagtaccatg atgcccgtgc gcagaagcag aatacagata cgctggaaaa acaaattaat
    19501 gagcgactcg aaggcgtgtt aagccgctgg ctgctgctga agaataacgt catgccagag
    19561 gcggcaaccg gtaccacggc cgaaaaataa ttatttaatt aaaggagatt atcatggcaa
    19621 atataagtta tttatctttg agtggagaga cacaagggct aatttcagca ggttgctcaa
    19681 cgcttgattc tgtcggaaac aaagcgcagc cagaacataa agatcatggt ttatgcgtta
    19741 atgcattcca tctcaagaag tcaaaatgtc aatcatcatg aattaattat caccaaaccg
    19801 gttgataaat cctcaccatt gctggcaaaa gcacttagtg acaatgaaaa aatggcaata
    19861 tgtgaattta tactctaccg aaccagtaaa gcgggtattt accagcctta ttataaaata
    19921 aatctgtcaa aagcccgaat atcaagtatt gattttgtca caccgcatgc tgtcctggaa
    19981 aaagaactag aaccacagga gagaattgcc tttatttatg aagatatttc ctgggagcat
    20041 acgttagccg gtacgaatgc aatgagtaaa tggcaggatc gtgtacagta acttactgta
    20101 ataacataat aaattactat tgcggagaga taaaatggct ggtttaagga ataacaacaa
    20161 cacacagaat gcacagtggg ctgattatgt gggtgacatt cttcgggggg ctcagccgat
    20221 aaaccagcta gtacctcagc acccttatct taacgacgtc ccactcattg atgagttaag
    20281 gcatcagaac actcatcatg ttattctctt gacacttgat gttgcaaaaa aaatactcag
    20341 cccaataaca tcatttgatt acattcattt tatcactaca catccttcag gtataaaaga
    20401 tacactcgcc tggttagtta acgcgagtaa attaatgact gagtttgatg acaatgggaa
    20461 aattatattt aaccttaatg ccttaaagta tacaaaagca agttactttg aaatacttgg
    20521 agagaaatat ataaaaatca caacatccag cccctggctt ttagaaaagc tcggtaaata
    20581 tattttctct tcacgagcgc ctcaagttct ggaactcgct attggctggc gaggagcttt
    20641 atctgaatca ataaaaggag ttaagttctg tatctggttt tcagtagcct ggagaaccat
    20701 agaatttatt atgagttcag agcgagatct ggttaatttt cttggcgatt tttcaatgga
    20761 tgtggccaaa gcagttatag ccggtggtgt tgcaaccgct attggaagcc tggcttcttt
    20821 tgcctgtgtt agctttggct ttccagtaat tcttgtcgga ggagcaattt tactgacagg
    20881 gatagtgtgt acagttgttt taaatgaaat cgatgctcaa tgccatttat cagaaaaatt
    20941 aaaatatgca attagagatg gactaaaacg gcaacaggaa cttgataaat ggaaaaggga
    21001 aaacatgact ccatttatgt atgttcttaa cactccaccc gtgatatgag gattaaccat
    21061 gcgtttacat gttaaattaa aagagtttct ttcgatgttt tttatggcca tattattttt
    21121 ccctgcattc aatgcttc
//
LOCUS       DAPHJE010000081        20741 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPHJE010000081 DAPHJE010000000
VERSION     DAPHJE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.081"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..1695)
                     /gene="actP"
                     /locus_tag="RH222_003010"
     CDS             complement(46..1695)
                     /gene="actP"
                     /locus_tag="RH222_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV3797997.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1692..2006)
                     /locus_tag="RH222_003011"
     CDS             complement(1692..2006)
                     /locus_tag="RH222_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV3797998.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2207..4165)
                     /gene="acs"
                     /locus_tag="RH222_003012"
     CDS             complement(2207..4165)
                     /gene="acs"
                     /locus_tag="RH222_003012"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV3797999.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4557..5993
                     /gene="nrfA"
                     /locus_tag="RH222_003013"
     CDS             4557..5993
                     /gene="nrfA"
                     /locus_tag="RH222_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV3798000.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPPKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6038..6604
                     /gene="nrfB"
                     /locus_tag="RH222_003014"
     CDS             6038..6604
                     /gene="nrfB"
                     /locus_tag="RH222_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV3798001.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6601..7272
                     /gene="nrfC"
                     /locus_tag="RH222_003015"
     CDS             6601..7272
                     /gene="nrfC"
                     /locus_tag="RH222_003015"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV3798002.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7269..8225
                     /gene="nrfD"
                     /locus_tag="RH222_003016"
     CDS             7269..8225
                     /gene="nrfD"
                     /locus_tag="RH222_003016"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV3798003.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8245..9963
                     /locus_tag="RH222_003017"
     CDS             8245..9963
                     /locus_tag="RH222_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV3798004.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9956..10339
                     /gene="nrfF"
                     /locus_tag="RH222_003018"
     CDS             9956..10339
                     /gene="nrfF"
                     /locus_tag="RH222_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV3798005.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10336..10932
                     /gene="nrfG"
                     /locus_tag="RH222_003019"
     CDS             10336..10932
                     /gene="nrfG"
                     /locus_tag="RH222_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV3798006.1"
                     /translation="MKQPKVPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11274..12587
                     /gene="gltP"
                     /locus_tag="RH222_003020"
     CDS             11274..12587
                     /gene="gltP"
                     /locus_tag="RH222_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV3798007.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12665..13354)
                     /locus_tag="RH222_003021"
     CDS             complement(12665..13354)
                     /locus_tag="RH222_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3798008.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13448..15595)
                     /gene="fdhF"
                     /locus_tag="RH222_003022"
     CDS             complement(13448..15595)
                     /gene="fdhF"
                     /locus_tag="RH222_003022"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15176..15178),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV3798009.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15793..17259)
                     /gene="mdtP"
                     /locus_tag="RH222_003023"
     CDS             complement(15793..17259)
                     /gene="mdtP"
                     /locus_tag="RH222_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV3798010.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDKVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17256..19307)
                     /gene="mdtO"
                     /locus_tag="RH222_003024"
     CDS             complement(17256..19307)
                     /gene="mdtO"
                     /locus_tag="RH222_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV3798011.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19307..20338)
                     /gene="mdtN"
                     /locus_tag="RH222_003025"
     CDS             complement(19307..20338)
                     /gene="mdtN"
                     /locus_tag="RH222_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV3798012.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20357..20632)
                     /locus_tag="RH222_003026"
     CDS             complement(20357..20632)
                     /locus_tag="RH222_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798013.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4673 a         5505 c         5685 g         4878 t
ORIGIN      
        1 agaggaagag tattccccgg tcaggcgacc ggggaagggg agagattaat gtgcgcggcc
       61 ttgctcaacg ccaaagccgg tctgggagcg gataaactgt gcacggaaca gttcacgctc
      121 gcgcgcacct tccgctgagt tatcggttgc cgagaagaac cagatgccga ggaatgccac
      181 gctgatagag aacagcgccg ggtattcata cgggaagatg gctttttcgt gaccaaggat
      241 ctgtacccaa atcgtcgggc cgaggatcat cagtaccact gcggtaatca gccccagcca
      301 gccacccatc atcgcgccac gagtggtcag tttcgaccag tacatagaaa gcagaatgat
      361 cgggaagtta cagctcgctg cgatggcaaa cgccaggcct accatgaagg cgatgttctg
      421 attctcaaac agcacgccga ggataatggc aatcacacct aaaatcagca cggtgatttt
      481 cgatacccgc agttcttcac gttcggtcgc gccttttttg aagacgttag cgtacaggtc
      541 atgcgaaacc gctgatgcgc ccgccagcgt cagaccagca accaccgcaa gaatggtggc
      601 aaaagcaacc gctgaaataa aaccgaggaa caggttgccg cccactgcat tcgccaggtg
      661 aacggccgcc atgttgttac caccaatcag atggcccgcc gcgtctttat attccggatt
      721 cgcaccaacc agcatgatcg cgccgaagcc gataataaag gtcagaatat agaagtagcc
      781 cataaatccg gtagcgtaga acacgctctt acgcgcttcg cgggcatcgc tgaccgtaaa
      841 gaagcgcatc agaatgtgcg gcaagccagc cgtaccaaac atcagtccca gacccagaga
      901 gagcgcggag atcggatctt tcaccagtcc gcccggtttc atgatgtcga cacctttcgg
      961 gtgtaccgcc atcgcttcgc tgaacagatt gttgaagcta aagccgacgt gtttcatcac
     1021 cataaaggcc ataaagctgg caccgaacag caacagcacg gctttgataa tttgtaccca
     1081 ggtggtcgcc agcatgccgc cgaacaggac gtacatcatc atcagcacgc cgaccagcac
     1141 caccgcaatg tgatagttaa ggccaaacag cagctcgatc agtttacctg cacccaccat
     1201 ctgggcgata aggtaaagcg ccaccaccac cagagaacca caggccgaaa gaatacgaat
     1261 cggcccttgt ttcagacggt aagaggccac atcggcaaag gtgtagcgcc ccaggttacg
     1321 cagacgttcg gcaatcagga acagaatgat cggccagccc accaggaagc ccagcgagta
     1381 aattaagccg tcatagccgg aggtaaacac cagcgcggag atccccaaaa atgaggcggc
     1441 ggacatatag tcaccggcaa tcgccagccc gttctggaag ccagtgatat tgccgcctgc
     1501 ggtgtagtag tcgctacgag aacgtacgcg ttttgatgcc cagtaggtaa tgccgagcgt
     1561 aaacacgacg aaaatcagga acataataat cgcctgccag ttcgttggct ggcgctctac
     1621 ggccccgcta atagcatccg cggcgttagc tgcgaaaggg agtgtggcgg caagcgccgt
     1681 cagaactctt ttcatgatgc ttgtacctca tgcaggactt cgttattaag acggtcgaat
     1741 tcgccgttcg cccgccagat gtagataccg gtgagaacaa aggagatcac aatcactcca
     1801 acaccaatcg gaataccgcg tgtgacgctg gtgttcggat tcagcggcgt gcccagccag
     1861 ccgggcgcga aggcgatcag taaaataaag ccgatataaa ctgccagcat aataatcgac
     1921 aggatggtgg caaaccgttg ccgtttttcg actaactccc tgaaatgcgc attgtcttct
     1981 atccgctgat aaatagtgcc attcatcaca gattctccag aggtaatgta gggattgttt
     2041 taattcccgc tcccttatgg gagaaggtta acgctcgggt aacccttgcc gaatgtaggc
     2101 cggataaggc gttatatgcc gcatccggca atcaatgcct gatgcgacgc tgtcgcgtct
     2161 tatcaggcct acaaaccgtt accgattcgc atccggcaat tgtgggttac gatggcatcg
     2221 cgatagcctg cttctcttca agcagcttct cgacaacgcc aggatcggca agcgtcgagg
     2281 tatcgcccag gttgctggta tcgcccgccg caattttgcg cagaatacgg cgcataattt
     2341 tgccggaacg ggttttaggc agggagtcgg tccagtgcag cacgtctggc gtcgccagcg
     2401 ggccaatctc tttacgcacc cagttgcgga cttctgcgta cagttctggt gacggttcct
     2461 ccccgtgatt aagcgtgacg taggcgtaga tcgcctgacc tttaatattg tgcggaatac
     2521 ctactacggc ggcttcggca atcttcggat gcgccaccag cgccgactca atctctgccg
     2581 tccccagacg gtgaccggag acgttcagca cgtcgtccac acgcccggtg atccagtaat
     2641 agccatcttc atcgcgacgc gcgccgtcgc cgctgaaata catatttttg aaggtggaga
     2701 agtaggtctg ttcaaaacgt tcgtgatcgc caaacagcgt acgcgcctga cccggccagg
     2761 agtcggtgat caccaggctg ccttcggtag cgccttccag cgggttacct tcgttatcga
     2821 ccagcgccgg ttgcacgccg aagaacggac gtgttgccga accggctttc agctcggtag
     2881 cgccaggcag cggcgtgatc atgaaaccgc cagtttcggt ctgccaccag gtatcgacca
     2941 ccggacattt ctcgttgccg atttttttcc agtaccactc ccacgcttct gggttaattg
     3001 gctcgcccac ggaaccgaga atgcgcagcg acgaacggtc ggtaccctcg atcgctttat
     3061 cgccttccgc catcagcgcg cggatcgccg tgggtgcggt atagagaata ttgacctgat
     3121 gcttgtccac cacctgcgcc atacgggcag gcgtcggcca gttgggtacg ccttcaaaca
     3181 tcagcgtggt cgcaccgcag gccagcgggc cgtacagcaa gtaactgtgt ccggtcaccc
     3241 agcccacatc ggcggtgcac cagtagatat cacccggatg ataatcaaag acatatttaa
     3301 aggtcagcgc cgcgtacacc agataaccgc cggtagtatg cagcacacct tttggcttac
     3361 cggtagaacc ggaggtgtag agaataaaca gcggatcttc ggcgttcatc tcttccgcct
     3421 ggtgctgatc gctcgcttgc tcaaccaggt cgtgccacca caggtcgcgc ccttcctgcc
     3481 agtcaatttt cccgccagta cgcttcagta ccaccacatg ctctacgctg gtgacgttcg
     3541 ggtttttcag cgcgtcatca acgtttttct tcagcggaat actgcgcccg gcacgcacac
     3601 cttcgtcgga agtgatcacc agtcgtgagt tggaatcaat aatgcgcccg gcaacggctt
     3661 ccggcgagaa gccgccgaaa atcaccgaat gcaccgcgcc aatgcgggcg caggccagca
     3721 tcgcaaccgc ggcttccggc accatcggca tataaatcgc caccacatca ccttttttaa
     3781 tgcccagctc gagcagggta ttggcgaagc ggcagacgtc gcggtgcagc tctttatagc
     3841 tgatatgttt gctctggctg gcgtcgtcgc cttcccagat gatggcggtg cgatcgccgt
     3901 tttcttgcag atggcggtca aggcagtttg ccgccagatt cagcgtgccg tcctcgtacc
     3961 atttaatgga cacattaccg ggggcaaagg aggtattttt caccttctgg tacggtttga
     4021 tccagtcgag aatttttccc tgttcgcccc agaaggtatc aggtacgtta atagattgtt
     4081 gatacatcgc ctcgtactgc tgagggttta tcaggcaacg gtctgcgatg ttggcaggaa
     4141 tggtgtgttt gtgaatttgg ctcatgcttt tgttctcctt gtaggatgtt aataatatgt
     4201 ggcataagcg ttaaatgtag gggtattggc agttttgttt agtatttggg cgacagatca
     4261 cgcaaaagta gaattgtgca aataaacggc agggtaattt ttgaaggtca ccaacaaaag
     4321 ttgattaatt ctttgaggaa catgcagtta tgcatgctgt tgaaaaagag gaagatactg
     4381 actaactcta aagtggtatt ttacatgcac ttacaattga ttaaagacaa cattcacagt
     4441 gtggttattt gttacacata ggggcgagca atgtcatgac agtgtaggtg cggttactgt
     4501 cgtgaaaaca ataaaaaccg ccattgcaac aatggcgcaa ttcggatgaa gcccctatga
     4561 caaggataaa aataaacgca cgccgtatct tcagcttatt gattcctttt ttctttttca
     4621 cttctgttca cgctgaacaa acggcagctc ccccaaaacc tgtaactgtg gaagcgaaga
     4681 atgaaacctt tgccccgcag catcccgatc aatatctctc ctggaaagcc acctcggaac
     4741 agtcagagcg tgttgacgcc ctggcggaag atccacggct ggtgatcctg tgggcggggt
     4801 atcccttctc gcgcgattac aacaagccgc gtggacatgc ctttgctgtg accgatgtgc
     4861 gtgaaaccct gcgtaccggt gcgccgaaaa acgctgaaga tggtccgcta ccgatggcgt
     4921 gctggagttg taaaagcccg gatgtggcgc gtctgatcca gaaagacggc gaagatggct
     4981 acttccacgg taagtgggcg cgcggcggcc cggaaatcgt caacaactta ggttgtgccg
     5041 actgccataa caccgcctca ccagagttcg ccaaaggcaa accggagtta accctttccc
     5101 gtccgtatgc ggctcgcgcg atggaagcca ttggtaaacc ttttgagaaa gccggacgtt
     5161 tcgaccagca atcgatggtt tgcggtcagt gccatgtgga gtattacttc gacggcaaaa
     5221 acaaagcggt taaattcccg tgggatgacg gcatgaaagt cgaaaatatg gagcagtatt
     5281 acgacaaaat tgccttctct gactggacta actccctgtc gaaaacgcca atgctgaaag
     5341 cgcagcaccc ggaatatgaa acctggacag cgggcattca cggtaaaaac aacgtgacct
     5401 gtatcgactg ccatatgcca aaagtgcaga acgccgaagg caaactctac accgaccata
     5461 aaattggtaa tccgtttgat aacttcgccc agacttgtgc gaactgccat acccaggaca
     5521 aagctgcctt gcaaaaagtg gtcgcggaac gtaagcagtc gattaacgac ctgaaaatca
     5581 aggttgaaga tcaactggtt cacgctcact tcgaagcgaa agcagcgctg gatgcaggcg
     5641 cgacggaagc cgaaatgaag ccaattcagg acgatatccg tcatgcccag tggcgctggg
     5701 atctggcgat cgcttcccac ggcattcata tgcacgcacc ggaagaaggt ttacggatgc
     5761 tcggtacggc gatggataaa gcggcggatg cacgcaccaa actggcgcgc ctgctggcga
     5821 ccaaaggcat cacccatgaa atccagatcc cggatatctc aaccaaagag aaagcccagc
     5881 aggccattgg cctgaacatg gaacaaatca aggccgagaa gcaggacttc atcaaaacgg
     5941 tgatcccgca gtgggaagaa caggcacgta aaaacggtct gttaagccaa taaccccgtt
     6001 ccgcctcgca aggggcggaa aacacaatgg agtgaatatg agcgtattac gttcgttatt
     6061 aactgccggg gtgctggcgt cgggcctgtt gtggagcctg aacggaatta ccgctacccc
     6121 tgcggcgcag gcatctgatg atcgctacga agttacccag cagcgtaacc cggatgccgc
     6181 ctgtctggac tgtcataagc cagataccga aggtatgcat ggaaaacatg cttccgttat
     6241 caacccgaat aacaaactgc cggtcacctg caccaactgt cacggccagc catcaccgca
     6301 acaccgcgaa ggggtgaaag atgtgatgcg ctttaacgag ccgatgtaca aggttggaga
     6361 gcagaacagc gtctgtatgt cctgtcatct gccggaacag ttgcaaaaag cgttctggcc
     6421 gcacgatgtc cacgtaacca aagtggcgtg cgccagctgc cattccctgc atccacagca
     6481 agatacgatg cagacgttaa gtgacaaagg acggattaag atttgcgtcg attgccacag
     6541 cgatcagcgc accaatccga actttaaccc ggcgtctgtt ccgttgctta aggagcagcc
     6601 atgacctggt ctcgtcgcca gtttctcacc ggcgtcggcg tgctggcagc cgtcagcggc
     6661 accgcagggc gcgtggtggc gaaaacgttg aatatcaatg gggtgcgtta cggcatggtg
     6721 catgacgagt cgttatgcat cggctgtacc gcctgtatgg atgcttgtcg ggaagtgaac
     6781 aaggtgccgg aaggcgtctc gcgcctgacg attattcgta gcgagccgca gggcgaattt
     6841 cctgatgtga aatatcgctt cttccgtaag tcttgccagc actgcgatca tgcgccgtgc
     6901 gttgacgtct gcccgaccgg tgcgtctttt cgtgatgccg ctagcgggat tgtcgatgtt
     6961 aatcctgatc tctgcgtcgg ttgtcagtac tgcatcgccg cctgtccgta ccgcgtgcgc
     7021 tttatccatc cggtcacgaa gacggcggac aaatgcgatt tctgccgtaa aaccaatttg
     7081 caggccggta agctgcccgc gtgcgttgag gcttgcccga cgaaggcgct gacgtttggc
     7141 aatctggacg atcctaacag tgagatttcg caactgctgc gccagaagcc tacttaccgc
     7201 tacaagctgg cgctgggaac caaaccgaag ctgtaccgcg taccgtttaa atacggggag
     7261 gtgagccaat gacgcagact tccgcatttc attttgaatc gctggtgtgg gactggccga
     7321 ttgccatcta cctgtttttg attggtattt ctgccggtct ggtgacgctg gccgtgctgt
     7381 tacgtcgctt ctacccgcag gcgggcggtg cagacagtac gttgctgcgc accacgctga
     7441 ttgtcgggcc gggcgcggtg atcctcggtc tgttgatcct cgtcttccac ctgacaagac
     7501 cgtggacctt ctggaagctg atgttccact acagttttac ctcggtgatg tcgatggggg
     7561 tgatgctgtt tcagctctac atggtggtgc tggtgctgtg gctggcgaaa atctttgaac
     7621 atgatttgct tgccctgcaa caacgctggt tgccgaagct ggggatcgtg caaaaggttc
     7681 tgagcctgct gacgcccgtt catcgcggac tggaaacatt gatgctggtg ttggcggtgt
     7741 tgttgggggc ttataccggc tttctgctgt cggcgctgaa atcgtatccg ttcctcaata
     7801 acccgatcct gccggtgctg ttcctcttct ccggcatctc gtccggtgcg gcggtggcgc
     7861 tgatcgccat ggcgatacgc caacgcagta acccgcattc cacggaagcg cagtttgtac
     7921 accgtatgga aatccccgtg gtatggggtg aaatcttcct gctggtggcg ttttttgtcg
     7981 gtctggcgct gggcgatgac ggtaaagtgc gtgcgctggt ggcggcatta ggtggcggtt
     8041 tctggacgtg gtggttctgg cttggtgtcg ccgggctggg gctgattgtg ccaatgttgc
     8101 tcaaaccgtg ggtcaatcgc agttccggca ttcctaccgt gttggcggcg tgtggggcca
     8161 gtctggtcgg cgtgttgatg ctgcgctttt tcattctcta cgccgggcag ttaacggtgg
     8221 cgtaagccag aaaagaggtg gtttctggac gtattccttc ctgaagtcgg ttttctggcg
     8281 ttgttgttaa gtctcggggt caacgtgttg accccgttga cggcctttgc gggagtgcgg
     8341 ttgcgctggc ctgccatgat gcgactcact tgcatcggca ttctggcgca gttcgcgctc
     8401 ctgctgctcg cctttggcgt actgacgtat tgttttctca tcagcgattt ctcggtcatt
     8461 tatgtcgccc aacatagcta cagcctgctg tcgtgggaac tcaaactggc ggcggtgtgg
     8521 ggcggccatg aaggttcgct gctgctttgg gtgctgctgc tttccgcctg gagcgcgctg
     8581 tttgcctggc attatcggca gcaaaccgat ccgctatttc cgctgacgct agccgtttta
     8641 tctctcatgc tcgccgcact gctactgttt gtagtgctgt ggtccgatcc cttcgtgcgg
     8701 atatttccac cagcaatcga aggccgcgat ctcaatccga tgctgcaaca tcccggtctt
     8761 atctttcatc caccgctgct ttaccttggc tatggcggtt tgatggtggc ggcgagcgtg
     8821 gcgctggcga gtttactgcg cggcgagttt gatggtgcct gcgccagaat ttgttggcgc
     8881 tgggcactac ctggctggag cgcattaacg gcggggatca tcctcggttc ctggtgggcc
     8941 tattgcgaac tgggctgggg cggctggtgg ttctgggatc cggtggaaaa cgcctcttta
     9001 ttaccctggc tttctgccac tgcgctgctg cacagtttgt ccctgacacg ccagcgggag
     9061 attttccgcc actggtcgct gttgctggcg atagtaactc tgatgctgtc gctactgggt
     9121 accttaattg tccgttctgg cattctggtt tcggttcatg cgttcgcact ggataacgtc
     9181 cgcgccgtgc cgttgttcag cctgtttgca ctgattagcc ttgcgtctct ggctctgtat
     9241 ggctggcgag cgcgggacgg tggtgcggtg gtgcgttttt cggggttatc gcgggaaatg
     9301 ttaatcctcg ctacgctgtt gctgttttgc gcagtgctac tgatcgtgct ggtgggaacg
     9361 ctttatccga tgatttacgg tctgctgggc tggggacgcc tctccgttgg cgcgccgtat
     9421 tttaaccgcg cgacgttacc gtttggcctg ttgatgctgg tggtgattgt gctggcgacg
     9481 tttgtctctg gcaaacgcgt gcagcttccg gcgctggtag ctcatgcggg cgtgctgtta
     9541 tttgccgcgg ggatcgtggt ttccagcgtc agccgtcagg agatcagcct gaatttacag
     9601 ccgggtcagc aggtgacgct ggcaggatac accttccgtt ttgagcgcct cgatctgcaa
     9661 gccaaaggca attacaccag cgaaaaagcg atagtggcac tgtttgacca tcagcaacgc
     9721 attggtgaac tgatgccgga gcggcgtttt tacgaagcac gtcgtcagca aatgatggaa
     9781 ccgtcaattc gctggaacgg catccatgac tggtatgcgg tcatgggtga aaaaaccgga
     9841 gcggatcgtt acgcttttcg tttgtatgta caaagcggtg tgcgctggat ctggggggga
     9901 ggattgttga tgattgcggg cgcattgtta agcggatggc gggggaagaa gcgcgatgaa
     9961 taaagggctt ctcacgctac tgctgttatt tacctgtttt gcgcgcgctc aggtcgtaga
    10021 cacctggcaa ttcgccaatc cacaacaaca gcaacaggcg ttaaatattg ccagccagtt
    10081 acgttgtccg cagtgccaga atcaaaactt actggaatcc aacgcgccgg tggcagtcag
    10141 tatgcgccat caggtttaca gcatggtggc ggaggggaag aacgaagtcg aaatcattgg
    10201 ctggatgacc gaacgctacg gagattttgt tcgctataac ccaccgttaa cgggtcagac
    10261 gctggtgtta tgggcgctgc cagtggtgtt gttactgctg atggcactga tcctctggcg
    10321 agtgagggcg aagcgatgaa acagcccaaa gtaccggtga aaatgctgac aacactcacg
    10381 atattgatgg tatttctctg tgtcggcagt tatctgttaa gcccaaaatg gcaggcggta
    10441 cgtgcggaat atcagcgtca gcgcgatccg ctgcatcagt ttgccagcca gcaaaccccg
    10501 gaagcgcagc ttcaggcatt gcaggataaa atccgcgcta atccacaaaa cagcgaacag
    10561 tgggcgttac tgggcgagta ctatctgtgg caaaacgatt acagcaattc gctgctggcg
    10621 taccgtcagg cgttgcaact acgtggtgag aacgctgaac tgtatgcggc gctggcgacg
    10681 gtgctttatt accaggccag ccagcatatg accgcccaga ctcgcgcaat gatcgataaa
    10741 gccctcgcgc tggactctaa cgaaatcacc gctctgatgc tgctggcttc cgatgcgttt
    10801 atgcaggcga attacgcgca agccatcgaa ctatggcaaa aagtgatgga tctcaactca
    10861 ccccggatca accggacaca gctggtcgag tcgattaata tggcgaaatt gttgcagcgg
    10921 agatcagatt aatcatctgg tgatgaagat tatgttattg agttgtataa aaaataatcg
    10981 aatgaacata tgccaaaaat aatcaccaat cagtattatt gcagattaaa caaataaaaa
    11041 tctttccata acaaatggtt attcattaat cctgccataa actcgcgtta ttatgcatta
    11101 atgcagcgaa aagctctgtt gttaaagggt tgcgcaacat accgcgcaaa tgatactgat
    11161 cataagcgtt aaaaaaatct acaaaccaac gcaacacaat tcatgccctg gcagtatgtc
    11221 acgttctcgc gtttctgaac ggggaacggc gctccattga ggaagttatt ctgatgaaaa
    11281 gtataaaatt cagcctggcc tggcagattc tgtttgctat ggtgctgggc attctcctgg
    11341 gaagctacct gcactaccat agcgacagcc gcgactggct ggtcgtcaat ttgctctctc
    11401 cggcgggtga tatcttcatc catctgatca aaatgattgt tgtgccgatt gtgatctcca
    11461 cgctggtggt gggtatcgcg ggtgttggtg atgccaaaca gctcgggcgt attggcgcga
    11521 aaaccattat ttacttcgag gtgatcacca ccgtcgccat cattttgggg atcactctgg
    11581 cgaacgtctt ccagcccggt gccggggtgg atatgtcgca gctggcgacc gtcgatatct
    11641 cgaaatatca gagcactacg gaagcggtac aaagcagttc ccacggcatt atgggcacga
    11701 ttttgtcgct ggtgccgacg aacattgtgg cgtcgatggc gaaaggcgaa atgctgccga
    11761 tcatcttctt ctcggtgctg tttggtctgg ggctttcctc cctgcccgcg acgcatcgtg
    11821 aaccgctggt gaccgtgttc cgctccatct ctgaaaccat gtttaaagtg actcacatgg
    11881 tgatgcgtta tgcaccggtg ggtgtgtttg cgctgattgc ggtgacggtg gctaactttg
    11941 gtttctcttc tctgtggcca ctggcgaaac tggtgctgct ggtgcatttc gccattctgt
    12001 tcttcgcgct ggtagtgctg ggaattgtgg cgcgcctgtg cgggttaagc gtctggatcc
    12061 tgattcgtat tctgaaagat gagctgattc tggcgtactc cactgccagc tctgaaagcg
    12121 tactgccgcg aattattgag aagatggaag cctacggagc accggcgtcg atcaccagct
    12181 tcgtggtgcc gaccggttac tcttttaacc ttgatggttc gacgctgtat caaagtattg
    12241 ccgctatttt catcgctcag ctgtatggca ttgacctgtc catctggcag gaaatcattc
    12301 tggtgctgac gctgatggtg acctcgaaag ggattgctgg cgtgccgggc gtgtcgtttg
    12361 tggtgctgct ggcaacgctg ggtagcgtag gtattccgct ggaaggcctg gcgtttattg
    12421 ctggtgttga ccgtatcctc gacatggcgc gtactgcgct gaacgtggtg ggtaatgcgc
    12481 tggcggtgct ggtgattgcc aagtgggaac acaaatttga ccgcaaaaag gcgctggctt
    12541 atgagcgtga agtgctgggc aaatttgata aaactgcgga tcaataattg aagattgccg
    12601 gggttatcca cccggcaatg tgtggatgcc tgatgcgacg ctggtgcgtc ttatcaggcc
    12661 taccttaacc gttggttaat ttttcaaact cttcacaccc ggtatcaaac ccttccatac
    12721 agctcaggtt caaccagtgc agcgcctttt gcttgttctt ctcgataaaa ccttcttcac
    12781 cgtttaagaa catcattccc gcccagtact cggaataacc ggttcgggaa attgcagagc
    12841 tgcgtttgaa ataccaggtt gccttgtcat cgtcggcctt aatgcccacg ccgttggcgt
    12901 aaatcaaacc aagcagcatt tgggcatcga cggcagagtc gttctccaga tcttccgagg
    12961 cgttttctaa cagcgaaatc gcctttggat aatccggttt acccgcttga gtatttacca
    13021 gaatatgcgc cagcgttact tcaccttctt tactgcccgc ttgcgccgct ttctctgcaa
    13081 gaacttttgc ttgtggatag tccagactga ccggattggt gattttgatc tgcgccagca
    13141 gcgcgcaggc atcagcatca ccgctgtcgg cggctttctg tgcccaatat tcggctttgc
    13201 tcaaatcgcc ggagctaaac cagctgtcgg caagaaaata ttgtgcgcgt cggtccccgg
    13261 cctctgctgc ctttaaatac tggctgccag gctcggagtc gttcgcgtgg gcaaagaatg
    13321 tcaaaaacaa catcaatgcg ataatttttt tcattttgat aatacgattg ggtagggagt
    13381 aaccagtata aagagttcga atggataaaa aaacagcctc cgaaaggagg ctgtagaaag
    13441 gactgtatta cgccagtgcc gcttcgcgca ggcgagtttt caacttgttg tactcgtcaa
    13501 tcacgtactg ctcggcggcg cgctgatcgg cgatcggctc gacgcgcacg gcgcagtatt
    13561 tgtattccgg cgttttcgta atcgggctta agttttcggt aaccagctcg ttacaggcac
    13621 caatccacca ctggtaggtc atgtaaatcg cccctttgtt cggacgatcg ctgacctgcg
    13681 cacgggtgat aattttgcct ttacgcgagt gcacccaaac caatgcctca tcttcaatac
    13741 ccagacgttt ggcgtcttcg gtattgattt gtgcgtagcc aggttcatca gccagcgccg
    13801 ccagtgccgc acagttgccg gtcatcgaac ggcaagagta gtgaccaact tcacgcaccg
    13861 ttgacagtac catcgggtac tcgtcggtga gtttgtcgat tggcgctacc cagtcgcagg
    13921 tgaagaactg tgccagaccg ttcggggtat caaacttctc tttaaacaga taagaagtcc
    13981 cctgatcggc atctgaagta tcgcggcaag gccactgaat gaagcccagt tcgcccattt
    14041 tctcgtaagt cgcaccgtag aaatccgggc acagatgacg caactcatcc cagatctcct
    14101 gggtgttgtt gtagtgcatc ggataaccca tacgggtggc gatttcactg atgatttgcc
    14161 agtccgtttt cagatcccat ttcggttcaa ctgctttgaa gaaacgctgg aagccacggt
    14221 cagccgcagt aaacacgcct tcatgctcgc cccacgacgt tgacggcaaa atgacatccg
    14281 ccgccgacgc ggttttggtc ataaagatgt cctgaacgat aaccagttcc agatcttcaa
    14341 aggctttacg tactgccgac agctccgcgt cagtttgcag cggatcttcg cccataatgt
    14401 acgcggcacg cacttcgcca tgcgctgcgc ggtgcggcag ctcgctgatg cgataaccgg
    14461 tatgagcagg caggctttcc acgccccagg ctttggcgaa tttctcgcgg ttagccggat
    14521 ctttcacgta ctggtatccc ggatacgtat ccggcagcgc gcccatatcg caggcaccct
    14581 gcacgttgtt ctgaccacga accgggttta cacccgcatg cggcttaccg aggttaccgg
    14641 tcagcatcgc gaggctggtc agagaacgca cggtttccac gccctggtag aactgggtta
    14701 cacccatgcc ccacaggatg gcggcgcttt tcgcctgggc atacatccgt gccgcctgac
    14761 gaatctcact ggcgctgacg ccggtgatat cttcaaccga ctccggcgtg tagccttcaa
    14821 cgattttacg atactcttca aagccttctg tacgggaagc gacgaacgct ttgtcgtaca
    14881 gattttcttc aataatgaca tggcccatcg cattcaacag cgcgatgttc gagccgtttt
    14941 tcagtgcaat gtgcatgtca gcaatgcgcg cggtttcaat tttgcgcgga tcgcagacga
    15001 taattttcgc cccgttacgt ttagcgttaa ttacgtgatt cgccacgatt gggtgggaat
    15061 ccgccgggtt gtacccgaaa acgaacacta aatcggtatt atcaatttcg ttaatagcat
    15121 tgctcattgc gccattaccg accgattggt gcagacctgc aaccgatggg ccgtgtcaga
    15181 cacgagcgca gcagtcaacg ttattggtac caataacggc gcgcgcaaat ttttgcatta
    15241 catagttggt ttcgttaccc gtaccacgcg aggagccggt cgtctggatg gcatccggac
    15301 cgtacttctc tttgatggcg ctcaggcgct cggcaacgta attcagtgcc tcatcccagg
    15361 aaacaggttc gagtttgccg ccacgctggc gacggatcat gggggttttc aggcgcgggg
    15421 tcaggatctg ggtatcgtta atgaaatccc agccataata acccttcaga cacagggtac
    15481 cctggttggt tttcccctgc gctgcctccg cccggacgat tttgccgtta tcgacgacca
    15541 ggttgatttt gcaacctgat gcgcaatagg ggcaaaccgt gacgactttt ttcatcggtc
    15601 tcgctccagt taatcaaatc acgcatacgc gctctcgact acagtatgca tcttttatgc
    15661 cacattttat ttggggtcat tccctgatat tacgggcact atttattcaa aactctgacg
    15721 aaaaacaggc tgtcgtcagt tttgacgtga cgaaacgaaa taccgcgtga cagccatcac
    15781 gcggcagaca ttttattttt tctcgacgac gggagccgcc tgatacccgc cgcccagcga
    15841 tttcatcaac tgaatgcttt ggatcacccg gcggctgtcc agcatcagta atgacatctc
    15901 ttcggcaagt actggcaacc gggcttcagt ggcctgtaag cggctggtta agccgcgctg
    15961 ataggcggcc tcggcagcgc gctgggtgaa gcgcgttgct tccacgcgtt cagcctgcat
    16021 ttctcgctcg tcgttgagcg tttgcagacg cgtgccgttg acggcaacgt cacgcaccgc
    16081 gttcagtact gactggttgt aacgttcaat catcatgttg ctggcggcgc gcgtgccttc
    16141 gagattggca ttcaaccgtc caccgtcaaa cagcggcaat ttcagacccg ggatgaagtt
    16201 gaactggcga ctggtttttt tgaataaggt atccaggtgg atggcatcaa ggccgaaaaa
    16261 cgctttgata tcaaagctcg ggtagaacag cgcccgcgcg gaatccacct tatctaatga
    16321 cgcctgaaca taccagcgca tggcttgcag atccgggcgt ctggcgagca actcataaga
    16381 gagtgtcgcc ggaatgccgg tctggactcg tggtaatgcc accggtttga tctccggcat
    16441 atcgctggcc cccgcgccaa tcaatgcacg cagagattct cgcgtttcgg tgatttgccc
    16501 tttgacggca gcaatttgtt tatcgaccgc cagaatctgt gcccgcgcgc cgtggaaagg
    16561 cacttgtgct tccagaccgt gcgccacttt actctggtgc gctttcaccg cgtaatcaat
    16621 cacatcgcga gtttgttcta acagatcgag catctgatag ctggcctgca tactgtaata
    16681 aagctgcgct acgcccgtgg tcagcgatag ctctactgct gcggtttctg ccagcgcggc
    16741 attatgcgcg ccaatggcgg cggcaaccgc tgagcgatgc acaccccaca aatcaagatc
    16801 cagtccggca aacaaaccta ctgtggcttc cgtatagtac ggcccgtcca tacccagtgc
    16861 gggcgcatcc atcgcataag ggcttaaaaa gccgttcgcc gagacgcgtt ggcggttgag
    16921 catccctaac gctgcgacct gtaattgtga accggcatct aacaaatcgg cctgggactg
    16981 cgctttttct tcccgcagtt tcgcttcggc gagggtgtgt gaaccactta gcgtccgttg
    17041 gatcagcgca tccagctgcg ggtcattgag ttgtttccac cactgcgcct gcggccagcc
    17101 ggagctggca agatgaatat cgtcggccag tttgatttgt tccggtttga gctgttgatg
    17161 aggtgcagaa tccttacgta ccagggcaca gccggaaatc agcgtcgtgc tgccgagaat
    17221 gctgcacaac agcagacgtg aaagttgacg attgatcatt gcgtggctcc ttgtgcctgt
    17281 tccgtggcgg gcgttaatgc cggggctgtc cagtctggca agcgggcgaa aagctggcag
    17341 acgtgttgct cctgttttaa taaggtgggc aggatggcct gcgaggcggg tgtctcttca
    17401 agcgttattt gaggagaacg gctgagtgcg gttgccagac cggcagcgta tttctccagg
    17461 gcgtcggcaa actgagctgc tctgtccggc tgtagtgcgt tatccagcgc ctgcgccgag
    17521 ttccaggtgg catcccaggc gtgaagaata tcgcggccct gacgaataac cgtttgcgaa
    17581 cgttcaatca gtaaggcgcg ttcttcgctg tccagttgac gctccagcgc cacgcgttgg
    17641 cacatctctt cacaggcatt aaacgccgca tgcagaccga tacgaatttg cagataagtg
    17701 cgcagagccg tgacttcctg ctggcgtggg atccgcatta ctttacttaa catacccagc
    17761 gtgccagcca gtttttgcgg cagtgtgcgc gcttcacttt caggccagac aaaggtgtaa
    17821 atcaccgcgg acaccacggt accaatgatg atacccatgg cgcgatcgcg aatttccacc
    17881 aggtcgtaca ccgggccaaa aacgttttcg agcgtggcga gcgcgaaggt gaccaccatc
    17941 tgtgtgccga tataagaaga gcgttcagag ctggtggcaa tccatgcgcc caacaggaaa
    18001 atcggtgcca gcacaaacag caattcgaca atattgtcca gccagggcat gaccagtagc
    18061 gtgaataaca gcgccagaat cgcgccgcaa aaggccccgc caaaacgcag caccatcttc
    18121 tggtacgacg aaccgacatt tgggttagcg acgatcacgc atgtcagcat acaggtgtga
    18181 atgccttccc agtccacgcc gctgtagaag gtgtaacaga tcaaacaggc gagcagtgtt
    18241 tttaccgcgt agcgcatata gtctggattg gtaaaagcat cggcgaccat tgatggcggt
    18301 ttggcggcgg gcgttggcgg cgtattcggg tccatctgac ccagttgtaa caacgtctgg
    18361 cagatattct ccaggttaca ttcccgtgcc gccatcgctt cactttcagt gatccgccag
    18421 tcgctttgcc agcactgacc ttcagcaacg gcatgctgca gcttattgat ttctgaggct
    18481 aatttttgcc gaaattcaat aattgcctga gaatcagcaa aagaggtggg atcgtagcga
    18541 ttcagcgtcg agtaaatgta ggttaccgtt gccacgcagc tttgccacca tgcgctttga
    18601 gttcgccagt tggcatcgtc cgcgagacaa aagacattca gtttttgcag cgccagcgcc
    18661 tctctttcaa tccgcgtttc gggtagcggt gcgaggctgt ccgtcaggtg actaatggca
    18721 tcatcaagcc gatcattaag cgcttgatgc atttgcgtaa tggcacgact gggaaaccac
    18781 agcacgccga ttaacgtcat cagcaaggtt ggatagaggc caacaacgat acaccacagc
    18841 gttaagcgca cgaccacttc cggatagtcg agcatggcgg ggaaggtttg cccgtaaatg
    18901 gcgacaatgg cgacagcgaa aaagaccagt cccagccgat gggtgcgcat caaaaacata
    18961 cagcccatca ggatcggccc ggcgatgatc aatcggatca acggttcgcc gtatgaccat
    19021 ttgtagatca aaaacaggct gccgatctcc agcaccgtgg caaccacaaa caagatcgcg
    19081 acaaatttgg tgtaaaacgc gttcgactga ataccgtaaa acagcactgc cagcgataac
    19141 gccacaaaag ggatctcaaa agtcatcgag atcagaatca ccagcaggca gcctacccag
    19201 agttgcacga tctgcggcac gcggccgggt cgccgctcgc ttaactcttc atgaaagaac
    19261 gccagcagcc tgaccaccgg taatggcagg gagttgagcg cgctcattat tgcggctcaa
    19321 gattagcgac tgccgaagcg ccgatgcgga acatttccgg gtcaggttta tcgaccatga
    19381 ttttgaccgg aaaacgctgg gcaacgcgga cccagttaat agagcgagaa actttcggca
    19441 ggccgcccag caccaggccg ccgtcatccg gtagcacgcc gtagccaatc gaatccactt
    19501 taccctcgaa ggttttgccg ctgtcactca tcaggcgaat cgttgcgggt gtacctgagc
    19561 gaatattttt cagatcggtt tcgcggaagt ttgcgatcac gtaccagtga cgagtgtcga
    19621 ttagggtaaa aatagggcgc atggcagaag caaattgccc gacggaggtt ttgagggaaa
    19681 tgacccggcc atcaaacggc gcgcgaacgg tcgccatttc cagatgaagt ttggtcaggg
    19741 caatatccgc ttcgaccgcc gcacgttgcg ccaccagcgc atcgacgccg ctgacggcgc
    19801 tggcggctga ctgcgcctgt aacaatacgg cattaagatc ggcttctgcg gcgcgctgcg
    19861 cagttcttgc acggtcaacg tcttccgctg agacaaaacc ctctttcagt aatggctcgg
    19921 tgcggcgtaa tgtatctgtg gcctgtttcg cggcggcacg ggctttttct accgtggcat
    19981 taatcgagtc ggcaccaaac tgttgcgcat caacgctacg ctgggtgagc ataatttgct
    20041 tatccagcgc cgcgagggag gcttcagctt tcgccagatt ggcttcgtac gggcgcgggt
    20101 caatgcggaa cagcaaatcg ccctgtttga ctgcctggtt gtcggtgacc gccagttcta
    20161 caatgcggcc gctgacttcc ggcaccacat caatggtatc tgctgacgcg taagcgtcat
    20221 tagttgatgg cgcactgtct acgcgccaga taacgaaaac aagggcaacc agcgccaatg
    20281 caaccactaa cagagcaggg aatttactgc gaggagcttt tttcggcgta ctttccatga
    20341 gcgcgtctca aatagattag aaaaatgcca gccagaacag catggcgtag agagcaaaaa
    20401 gggcggtata aataattccg acaaatgcca gattgatatt tgcccgctga ataactcgcc
    20461 tcgtgatgag catcaaaata aggctggcaa tggcgcagaa aaaccagctg ggataataag
    20521 caccgatcac cgggattgcc ggagataatg agcatcccga aaccatgaag acgggaatta
    20581 tccaggctgt ttttttgagt tgcatcgcca tgcgagagag aactgttggc atattaaata
    20641 catcctgtct gctgcgaaca atatctgtag tgaatatcaa tatccattga aggcgtcagt
    20701 atggctattt tctcgatgaa taaaaatagc tttattcagc c
//
LOCUS       DAPHJE010000082        20076 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPHJE010000082 DAPHJE010000000
VERSION     DAPHJE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.082"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(147..893)
                     /gene="cutC"
                     /locus_tag="RH222_003027"
     CDS             complement(147..893)
                     /gene="cutC"
                     /locus_tag="RH222_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV3798014.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(907..1473)
                     /locus_tag="RH222_003028"
     CDS             complement(907..1473)
                     /locus_tag="RH222_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3798015.1"
                     /translation="MANWQSIDELQDIASDLPRFIHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1689..3422
                     /gene="argS"
                     /locus_tag="RH222_003029"
     CDS             1689..3422
                     /gene="argS"
                     /locus_tag="RH222_003029"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV3798016.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3599..4087
                     /locus_tag="RH222_003030"
     CDS             3599..4087
                     /locus_tag="RH222_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV3798017.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DVNVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4207..4599)
                     /gene="flhe"
                     /locus_tag="RH222_003031"
     CDS             complement(4207..4599)
                     /gene="flhe"
                     /locus_tag="RH222_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV3798018.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4599..6677)
                     /gene="flhA"
                     /locus_tag="RH222_003032"
     CDS             complement(4599..6677)
                     /gene="flhA"
                     /locus_tag="RH222_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV3798019.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NIVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6670..7818)
                     /gene="flhB"
                     /locus_tag="RH222_003033"
     CDS             complement(6670..7818)
                     /gene="flhB"
                     /locus_tag="RH222_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV3798020.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFCGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8020..8664)
                     /gene="cheZ"
                     /locus_tag="RH222_003034"
     CDS             complement(8020..8664)
                     /gene="cheZ"
                     /locus_tag="RH222_003034"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV3798021.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8675..9064)
                     /gene="cheY"
                     /locus_tag="RH222_003035"
     CDS             complement(8675..9064)
                     /gene="cheY"
                     /locus_tag="RH222_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV3798022.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9079..10128)
                     /gene="cheB"
                     /locus_tag="RH222_003036"
     CDS             complement(9079..10128)
                     /gene="cheB"
                     /locus_tag="RH222_003036"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV3798023.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYNEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10131..10991)
                     /gene="cheR"
                     /locus_tag="RH222_003037"
     CDS             complement(10131..10991)
                     /gene="cheR"
                     /locus_tag="RH222_003037"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV3798024.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11010..12611)
                     /gene="tap"
                     /locus_tag="RH222_003038"
     CDS             complement(11010..12611)
                     /gene="tap"
                     /locus_tag="RH222_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV3798025.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAL
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESVQLQIAPVVS"
     gene            complement(12657..14318)
                     /gene="tar"
                     /locus_tag="RH222_003039"
     CDS             complement(12657..14318)
                     /gene="tar"
                     /locus_tag="RH222_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDV3798026.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14463..14966)
                     /gene="cheW"
                     /locus_tag="RH222_003040"
     CDS             complement(14463..14966)
                     /gene="cheW"
                     /locus_tag="RH222_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV3798027.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14987..16951)
                     /gene="cheA"
                     /locus_tag="RH222_003041"
     CDS             complement(14987..16951)
                     /gene="cheA"
                     /locus_tag="RH222_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV3798028.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16956..17882)
                     /gene="motB"
                     /locus_tag="RH222_003042"
     CDS             complement(16956..17882)
                     /gene="motB"
                     /locus_tag="RH222_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV3798029.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17879..18766)
                     /gene="motA"
                     /locus_tag="RH222_003043"
     CDS             complement(17879..18766)
                     /gene="motA"
                     /locus_tag="RH222_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3798030.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18893..19471)
                     /gene="flhC"
                     /locus_tag="RH222_003044"
     CDS             complement(18893..19471)
                     /gene="flhC"
                     /locus_tag="RH222_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV3798031.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETHLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWLFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19474..19824)
                     /gene="flhD"
                     /locus_tag="RH222_003045"
     CDS             complement(19474..19824)
                     /gene="flhD"
                     /locus_tag="RH222_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV3798032.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4773 a         5548 c         4924 g         4831 t
ORIGIN      
        1 cgctatgcgc actgtcaccc gctcaacaga tcacggttat cgttcgtttt ttatactgtt
       61 cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca agcatcatat
      121 tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat gattcctttc
      181 atttcagcaa ccgccgcccc gtctacgata taacgcgaat actcgtccgc gtgttcatct
      241 gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca cgctcccgcg
      301 gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc tgcacggact
      361 cctgctccgg ccataatgat tggagcatca cgatgggcaa taagttccat aatttttgat
      421 aaaccttgca gtgcgtctga tttttgccct gatgtcagta cccgggcaat gcccaattcc
      481 gcaagattat tgagtgtata taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc
      541 actgccagcg gaccggcagc agccattatt ttttccattc gtggcatatc gacattcccg
      601 tcaacatcga gaacgcccgt caccagtcca ggaaaaccta attcgcggac cgtgcgcaca
      661 tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga
      721 attatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac acccaacgac
      781 ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc gccgttttgc
      841 tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc catttttact
      901 ccttaattac gccgactgct cgctggcaac gatctcttca atggaccacg gatgaaactt
      961 aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct catgctcacc
     1021 ttttggcgaa ctggttttca ctgagatccc cggtaggctg agtccttcat cagaaagcaa
     1081 tgccagcgca cgggcattca gcgtttctgg atcacccggc agaacgattt caatatgttc
     1141 ccaaccttcg tgtgggtaac gtttttcccc gggccacggt agctccacaa tagaaaactg
     1201 ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc tgccattgat
     1261 catattttct gacaaaagct cgccacactg ttcaaacccg cgacgccagc gttcagcagt
     1321 ggcgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga tattcaaccc
     1381 cagacggcgg gaaagttcat ctaatgcgtg gataaatcgc ggtaaatccg atgcaatatc
     1441 ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt cagtaaaagc
     1501 gttaatttac cctgttgccc tgtgccaacc aaccgctgat ttcacgccgc ttctgatgca
     1561 atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca tttgcagtat
     1621 actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca tcaatgtaag
     1681 gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg
     1741 caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg
     1801 actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca ccgcgacaat
     1861 tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa gttgagatcg
     1921 ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa catgttcagc
     1981 aggcgctggc gtccgatcgt ctcggtgttg ctacgccaga aaaacagacc attgtggttg
     2041 actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg tcacctgcgc tctaccatta
     2101 ttggtgacgc agcagtgcgt actctggagt tcctcggtca caaagtgatt cgcgcaaacc
     2161 acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa aagcagcagc
     2221 aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc gatgcgaaaa
     2281 agcattacga tgaagatgaa gagttcgccg agcgcgcacg taactacgtg gtaaaactgc
     2341 aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc
     2401 agaaccagat cacctacgat cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg
     2461 aaagcctcta caacccgatg ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg
     2521 cagtagaaag cgaaggggcg accgtcgtat tccttgatga gtttaaaaac aaggaaggcg
     2581 aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc accactgata
     2641 tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg tattacatcg
     2701 actcccgtca gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg
     2761 taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt aaagacggca
     2821 aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc cgatctgctg gatgaagccc
     2881 tggaacgtgc acgccgtctg gtggcagaaa agaacccgga tatgccagcc gacgagctgg
     2941 aaaaactggc taacgcggtt ggtattggtg cggtgaaata tgcggatctc tccaaaaacc
     3001 gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt aataccgcgc
     3061 catacatgca gtatgcatac acgcgtgtat tgtccgtgtt ccgtaaagca gaaattgacg
     3121 aagagcaact ggctgcagct ccggttatca tccgtgaaga tcgtgaagcg caactggcag
     3181 ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg
     3241 taatgtgtgc ttacctgtac gatctggccg gtctgttctc tggcttctac gagcactgcc
     3301 cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctaaaactg gcacaactga
     3361 cggcgaagac gctgaagctg ggtctggata cgctgggtat tgagactgta gagcgtatgt
     3421 aatcgatttt tcgtgagagt gaagcctgat cagggttagc cgatcaggct tttttattgc
     3481 catctaaatg tattctgaaa atggacatgc cattgttttc tcactgttgg ataagaggcc
     3541 agaagcgtaa tatccggccc cagggaaacg ataacggttg aatttaagga ataccgcagt
     3601 gtttaaattt cttgtattaa cattaggcat tatctcttgc caggcttacg cagaagatac
     3661 ggttatagta aacgaccatg acatttcagc catcaaagat tgttggcaaa aaaattcaga
     3721 tgatgatact gacgttaacg tgatcaaatc atgcctgcga caagaataca atctcgtcga
     3781 tgcgcagtta aataaagcct atggtgaagc ttatcgttat atagaacaag tgccacgcac
     3841 aggtgtaaaa aaacctgata ccgaacaact taacttgctt aaaaaatcac agcgagcctg
     3901 gctggatttt agggacaaag aatgtgaatt aatcctttca aatgaggacg ttcaggattt
     3961 aagtgaccct tattctgaat cagaatggct ctcatgtatg atcatacaga ccaatacgcg
     4021 aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc ctttgacaag
     4081 aggataattc acatcttttt ggcatgtttt gttgcaagct attcctgata aataattgca
     4141 acaagacatc gagccttttt cactgagtta ttaaacatac tcgcgagcgc gtaatttttt
     4201 tgtccttcag cgataattca caatcacttc attacgttgt acctttagcg gtggaattaa
     4261 ccgcccaccg cctggcactt cccagataaa tcgcaacggt tctgctgccg ctataccgga
     4321 aaaggccacg gtggttccgc tctgcccctc taattcgaca caacgagatt gcgaacacaa
     4381 gcgaacccgc agtcctgaag gtgtcgggcc gataagctga taacgccacg ctaccagcgt
     4441 catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca gacgacatcg actcaccgcg
     4501 atgatttagc gtaattccta cactacttgc ctgccacatc ccctccccgg cggcttgcac
     4561 cagcagcgga aacaataata ttgctaataa ggttctcatt atttgccgcc aattgtcgcc
     4621 gtcatgcgga tatgtcggtt atcagacagt tccagattcg acaggaccac taactgcggc
     4681 aagctgcggc gcaggaagcg agacaataat ggtcgcagcg cgtggttcac caacaatact
     4741 ggcggcgcac ccagcatctc ctgacgggat agcgcttcct gagtttgcgc cagtaaacga
     4801 tccgccagcc ctggctccag tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc
     4861 agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca ctgctgggta
     4921 atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt catgtggatc gctttggatg
     4981 ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca
     5041 tcgaggagat tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga
     5101 tcttccgtca gctttggcat ctcctgggcg acgcgatcca acagctgttg cgcctcctga
     5161 cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt tgctaccacc
     5221 gtgctggcct caaccactgt gtacccctga atctgcgcct gttcttttag cgcactttca
     5281 atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg taacgtcccg
     5341 gcagcggttc cagggttaat cgccagccag cgccccggat aagcatcacc actgccaatc
     5401 tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg
     5461 tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt tcttgcggat actgcgtata
     5521 cggcccaaca actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg ataacccact
     5581 tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac aacggtatta
     5641 ttctctgcca tttttaccgg tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc
     5701 caccaggcca gcccgagcaa tccggcagtg aacagcaaaa ataccaggtt cggcattcca
     5761 ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca acataacgct tgggttactg
     5821 aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg
     5881 acccccgcgg cggtagaaat caccagcgcc ggaatttgtg ccaccagacc gtcgccaatg
     5941 gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg cagcacgccg
     6001 accagcaacc cgccgacaat gttaatgacc atgatgagga tcccggcgat ggcatcgccg
     6061 cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg agtcacttcg
     6121 gagcggcgtt ttttcgcctc atcttcacca atcaatccgg cgttaaggtc ggcgtcaatc
     6181 gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc tgcgatacgc
     6241 ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacaaa caccacgata
     6301 ccgatagcga aattgccacc aacgaggaag tgaccgaacg cttcgaccac cttccctgcc
     6361 gccgccgcgc cggtatgccc ttccattaaa atgatacggg ttgaagccac gttaagtgcc
     6421 agacgcaaca gcgtggtaaa caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc
     6481 tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa ggtaaacaac
     6541 aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa caggatcaaa
     6601 atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc
     6661 gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt ccggcaccgg
     6721 aagatgagta ggttgtacag ggcgctgtcc accagccaga cgccagcgtt tcagttgcca
     6781 gacccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt gttgaccaat
     6841 ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt
     6901 ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct gcacctttag cgaccacttt
     6961 cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg tcgggttatt
     7021 gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcag cagctcgctg
     7081 catctgacgg atccgccctt taacatgagg gtcaccttcg ctttgtttga actcatcacg
     7141 aatatcctgc cgtgacatac gcagcttttt caggtggctg aatatttgga aaaagacgtc
     7201 aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc ctaccaaatc
     7261 catcgcatta cccatggcgg taatcggaga ctcggccatc aagcgcatca tctgcggcca
     7321 gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg atggttttca aaattgcttt
     7381 aagcaactcc gcgccagtct gagccgagaa catccgttta atgcccggta gcgggttgag
     7441 tttggaaaac ttcggctgca aggatttgcc gcaaaatacc agccctccca gcatgaccgg
     7501 agaaatgagc gccaccagca ccacgccgct aatcagcggc agcagcgcca gcatggcttc
     7561 tctgatcagc agaataatct gcccgaggat cagattcggg tcattgataa tactgtgatc
     7621 aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca caccgccaaa
     7681 ccagataaca ctaacgccca ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg
     7741 gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggctt ctgttttgtc
     7801 gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcgtga atgatgccag
     7861 agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag gcgggattta gggcttttgc
     7921 tgccacacat caagcatagt gtgcgtttgt cggatgcggc gtcatcgcct tatcagaccg
     7981 cctgatatga cgtggtcacg ccacatcagg caatacaaat caaaatccaa gactatccaa
     8041 caaatcgtcc acctgatcct gactggctac cacaccggct ttgctggtat cgacctgagg
     8101 tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg ggatgttttc
     8161 caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca tccgcttaat
     8221 gacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca gttgcgcgtt
     8281 agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt cgtgtatctg ttaccagttc
     8341 acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt gggttaacgc
     8401 ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac tgttcagcgc
     8461 ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc gcgcatcggg
     8521 gatggcttcc gccgcttcgg caatggcctg atccagcccc agttcccgca aactgtcgcg
     8581 cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc
     8641 aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc tcaaagattt
     8701 tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag ccactggccc
     8761 ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc attaacactg
     8821 gcaatgccga catcgcgcca tccgcacgaa ttgttttcag caattccagg ccatccatat
     8881 tgggcatgtt ccagtcggag ataacaaatc cataaccgcc tgcctgcaac ttattgagag
     8941 cgtcgacgcc atcttccgct tcctcaacat tattgaatcc cagctctttc agcaggttac
     9001 gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt tctttatccg
     9061 ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattt ttgccaacat
     9121 ttgctggctt acctggctaa gatcgaccac ttcgcagaca ccacccatat tgatggcctc
     9181 gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc
     9241 ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc
     9301 aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta ccgaaggccg
     9361 atgacggtta accgccgggc catcgtgaat tttgatttgg taatttgcgc cactacgcgc
     9421 cagctccata tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga cacgttctcc
     9481 gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg caaaagagcg
     9541 ggtgaaaccg ggcggcatat gctgggtaat taacagtgcc gggctggaaa gcggcaacgg
     9601 ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg caatcagttt
     9661 ttcagaactc aacaacggcc ccgccttcag cgttgtcggt gccgacaatg gcttatgtgc
     9721 tgcaaggctc gcctttgctg ccgtacgcac cttttcagca atcatttcgt tatacgccag
     9781 cataccttcg cgaataccca gttgcggttt ggtgacaaaa tctatcgccc ccagctccag
     9841 cgcgcgcagc gtgacttctg accctttgcc ggtcagggaa gaaaccatca caacgggcat
     9901 tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg gcatttcaac
     9961 atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcga ccagcggatc
    10021 aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg tcatgatctg
    10081 gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg ttaatcctta
    10141 cttagcgcat acaccgtctg accacgcagc gtgaagcggc gctcaaggtg gctaaagttt
    10201 tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa
    10261 atctcctgct gggtagtttg atcgaagtag atcatgacgt tacgacagaa gatcgcatca
    10321 aacggccccg gcacggtgta ctgtttcgcc agtagattca gcggggcaaa atcaacatag
    10381 ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gccccgtccc tcgcatgaaa
    10441 taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata gataccgctt
    10501 ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca gcgtccgggc
    10561 gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc gccggtcgaa
    10621 gccgccgcgc tccatacgcg atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga
    10681 gggaaatgat gtgcctcacg gaaaaatgcc gtcagattcg tggtcagcga attgataaac
    10741 gcctgccact caccgctgtg ctgattagat tccagcaagt tcagataatg accgaaatcc
    10801 gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg
    10861 tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg aaaatgcgcg
    10921 tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc acagggcaga
    10981 gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat ttgtaactgc
    11041 accgactcat gtcgtgctac ttcatgttct tcaagggtaa ataccgccac acgcgacgaa
    11101 agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc taccagcgag
    11161 gcgttctgct gagtcacctg atccatctgg ctgacagcct gtgcaacctg ctcaatcccc
    11221 cgctgttgtt cttccgacgc agaggcaatt tctcccataa tgtcgttcac gcgggtcacc
    11281 gaactgacaa tatcgatcat ggtcgcggcg gcgttattca ccagtttcga accctgctgg
    11341 acacgattga ctgactcttc gatcagccct ttgatctctt ttgctgcctg cgcgctacgg
    11401 ctggcaagat tgcgcacttc acctgccact accgcaaaac cacgcccctg ctctccggcg
    11461 cgagccgctt ccactgccgc attcagggcc agaatattgg tctggaaagc aattccgtcg
    11521 ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg catggtgtga
    11581 gtcatggtac tgacctgaac accgcccgcc agcgccgttg tcgcggcatt ttttgccagt
    11641 tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa ttgctccata
    11701 ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga gagatcgtta
    11761 ttgcctgcga caatctccgc gataccaatg tgcatctcct ggcttccctt acgcacatca
    11821 cttaccgtcc cacgcaaagc ctgctgcatg gtcttcagac tggcaaaaat ggcggtgatc
    11881 tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat gctgtcaaaa
    11941 tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca cagcgcactg
    12001 ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagtgccga aatctgatag
    12061 ttacgctggc tttgcgcact ggcggcttcc agcacatggt tgatctccag ctgccaggcc
    12121 tcaaagttaa cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa
    12181 agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa
    12241 cttttctccg tctctttttg caatcccctg acgtgctcgt tgcccgcagt catcgccata
    12301 aaacttttaa acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt
    12361 ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc ccgctttgtt cagcgcggta
    12421 ctggcctgta acattaccgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc
    12481 tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg
    12541 ccaatctgca agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata
    12601 cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa
    12661 aatgtttccc agtttggatc ttgttcagca attcgcagtc gtggctgtgc cggtggttgc
    12721 tcactggcag gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacgg
    12781 aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt ccagcgcagc ggcggcggca
    12841 gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc
    12901 aatgcgactt gatcgatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata
    12961 atgtcagtca cgcgagtgac agcattgacg atattgttca ttgtttcccc ggcgctttcg
    13021 accagcaccg aaccggtatc aacgcgtgag acggagtctt caatgagggc tttgatctct
    13081 tttgccgcct gggcgctgcg actggcaaga ttacgcactt cacccgccac cacggcaaaa
    13141 ccacggccct gttcacccgc acgcgcggct tcaaccgcgg cattcagcgc gaggatatta
    13201 gtctggaagg caataccgtc gataacgctg ataatgtcgg caattttctt cgaactatcg
    13261 gcgatctcat gcatcgtttt cactacgcca tccaccactt tgccgccgtg ctgggcggtg
    13321 tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact
    13381 gtcgcggtga gctgctccat gctggcggca gtttcttcca gcgcggatgc ctgctgttca
    13441 gtacgggagg aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca
    13501 tctgaacctt cgcggacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg
    13561 ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta
    13621 ccaccggcga tttcgcgaat gtgagcaata atttttgcca gcggagtaag caacatacgg
    13681 cgaatgccgt accacgccac cagcagaatc aataccacca ccagcgcgat aaccgccagt
    13741 tgccactggg caaatcggta atcatctgcg ttgtcagtga cgatatcgcg atacagtttt
    13801 tcactgctga gggcgtactg agcaaacgct tcgcccattg cattttgcat tccctgggtt
    13861 ggctgagcga aataagctcc agtattgcca taatctagat aatcaatcag ttcagttaac
    13921 gctgtgtaat agtttttata tttttcatca atattacgac tggtagcgac catttcaggt
    13981 aacggtgcca tgcttttgaa ttttttataa tgcgtcgctg cctgcgccaa tgttttcctg
    14041 gcgctatcga gcaattcaac tttggcgtta ctttgttgat tggaggaatc catcatcatc
    14101 cgtaccgctg aacgactcag gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc
    14161 agctcgccct gctgttcccg taattgattg gaaaccacaa agctcttctg gctatggtga
    14221 agggaagaaa aaaacagact gccggaaata agctgtaaca gtgcgaatac ccccagcacc
    14281 attaccaaca gcgtgactac gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag
    14341 ttgatctcgt tatcggcaag gaggggggaa actttattgc tgatgccacc cgccgcgaaa
    14401 ttgaaataaa aaacccgatg cgcagatcat cgggttcatt tcaattgagg aaatcgggag
    14461 aattacgcca cttctgacgc cgcgctatct aacagcgcca tctcttcgct gttcagcagt
    14521 ttttcgatgt tcaccagaat caacatccgg tcgcccagtg cgcccagtcc agtgagatat
    14581 tctgttgaaa gcgtcacggc aaattccggt gccggacgaa tttgctccgc cgtcaatgaa
    14641 agcacgtctg agacgccgtc aaccacgatg ccgaccaccc gctgtccgag attcaggacg
    14701 ataactaccg tgttgtcgtt atagtccaca tccacctggc tgaacttaat tcgtaagtca
    14761 acaatcggca caataacgcc gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc
    14821 gcaatccgtg ttacctgatc gtagccacgg atctcctgca ctttcaggat atcaataccg
    14881 tactcttcat caccaagggt aaataccaga aattcctggc ctgacggctc gctggccagc
    14941 tttgttacat tcgtcatacc ggtcatattg ttaccttttt actcattcag gcggcggtgt
    15001 tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga gacatcaaca atcagtgcca
    15061 cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg gactttgcga tagttacttt
    15121 caaggttttt aaccacaacc tggtgttgac caattaattg atccaccagc aaggcgtagc
    15181 ggcgaccgcc actttgtaag atcaccacaa ttccctgggt ggcttcggtt ttcgcgcccg
    15241 cgacgttgaa cactttccac agttcgacga tgggcagata ttcaccccgc acttccagca
    15301 cccgctcgcc gccggccagt ggatggagat cggcttcacg gggttgcagt gattccataa
    15361 cagcattcag cggcagaatg aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga
    15421 tggccagcgt cagcggcagt aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga
    15481 tttcgacatg accgcccatc tcctggatat tacgtttaac gacgtccatg ccgacgccgc
    15541 gcccggagac gtcggtgacc tgctctgccg tggagaagcc aggtgcaaat atcagcatcg
    15601 cgacttcgtc gtcgctcatg ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca
    15661 gaattcgctc acggtttagc cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc
    15721 cctgatgttc ggcagacaga attaaatttc cgacgctgtt tttacctgcg gcgagccgtt
    15781 tttctggcag ttcaataccg tgatcgaggc tattgcgtac caggtgggtc agcgggtcga
    15841 taatgcgttc tatcaggctt ttgtcgagtt cagtagaact gcccaccagc gtcagttcta
    15901 cctgcttgcc gagttttccc gccagatcac gcaccagccg gggatagcga ctaaaaacat
    15961 attccatcgg catcatgcga atcgacatca ctgattcctg caaatcacgg gcgttacgtt
    16021 gtaactgccc catgctggtt atcaaatcac catgattaac cgggtccagt tcgctggaac
    16081 gctgggcaag catggactgg gtgataacca gctcgccgac gaggttaatt aattgatcaa
    16141 ccttttctac cgctacacgg atgctggtgg attcattgct gcgcgtcgtt ttttcccgct
    16201 ccacgcggcc ggttggcgct tgttcggctg ccagtttaag cactggtggg gtggatattt
    16261 ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta
    16321 ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat tgccgagagc gaatccgccc
    16381 ctttcaccac gtcagttaac gttgtcagat gtcccagttc ttcttccagc aggtcgactt
    16441 ccccggcctt caggcgcgaa aggataattc gtcgcggcga ctgactgcga ctctgctcat
    16501 cttgcggttc acttttggca accacactta atcgggtcac tgcggatggc gtttcgcctt
    16561 tcgcttctaa tgccagttga cgcaaggcct ggcagatata atcgaagctg gcggcatccg
    16621 gctcttgcga ctgtttataa gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa
    16681 acagattgat aatgtcggtg ttgagttgca tctcacctcg tctggcttca tcgagcaggt
    16741 tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt
    16801 tgatcgagtg ggcagcccga aagatggcat tcaattgttc ggcatctggc gcttccggct
    16861 gcaaaaccag caaatgctgc tccatgtcag ccaacagttc gtccgcttca tcaaaaaatg
    16921 tctgataaaa atcgcttata tccatgctca cgctgtcacc tcggttcggc tgatggcatt
    16981 gtgggaacac tgacctgtgg tgcaacctca ggtttttcca gggcgcttac tggctcattc
    17041 tggctttcgg cgttttcatg caaaatggcc tgttcggctt gtttgttcag taccagcagg
    17101 ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc
    17161 atgccgacga cacgtaacac tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg
    17221 gatgcattgg cccgatcggc agaaagctcc cagttgctat atcctttctc accgctggcg
    17281 taggggaaat catcggtatg acctgaaagg ctaatacggt tgggaatacc gttcagtaca
    17341 ggcgcaatgg cgcgcagaat gtcgcgcata tagggttcga catcggcact gccggttcta
    17401 aacatcgggc gattctggct atcgatgatc tgaatacgta gaccttcctg gaccagatcg
    17461 attttgagat ggggacgtaa cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga
    17521 tcaccccgca atttccgcaa tcgactttgc tccatgcgtt ttttcagctc ttcgatgttc
    17581 ggctgcttat tcacttcccc ctggctttgg gtgtaatcat caccaccgcc gggaattggg
    17641 ctttcactat tagaaatgcg atcgccgccc gtaaccgcag tcgccagtgg agtccggaag
    17701 tactccgcaa tctgaatcag ctcttttggg ctggagatgg agatcagcca catcaccaga
    17761 aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa tcttccacga tccatgtgct
    17821 gccccgtggc ttttggcttt gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc
    17881 atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac cgcacgcaca tgctcttcca
    17941 gttcaataaa cgacggacgt tcgctggaat agagcgtttt gcgaccaaac tcaacggcga
    18001 taggcggtgc gtaaccgttc agattagaaa gcagagtgac tttgacgcac tgcatcattt
    18061 tgctggtttc ggcgcttttc tgacgtaaaa cagtcgctaa tggggaaata aatccgtaag
    18121 ccaataaaat gccgaggaaa gtccccacca tcgcatgtgc gataagcgca cccagctcgg
    18181 cggcaggacg atcggctgaa cctaacgcgt gaacgacccc cattacagcc gcaacaatac
    18241 caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt cgccgggact tctgcctcgc
    18301 tttcgtgcgt ctcaatctct tcatccatca gggcttcgat ttcgaaggtg ttcatgtgac
    18361 cgctgataat caggcgcaga taatcgacga taaaatcaag catgacgcta tccgcgagga
    18421 tgcgtgggta gctggcgaag atctcgctct cacggggatt ttcaatatca cgttccagcg
    18481 aaaacatccc catctgccgc gatttcgcca tcaaccgata aagcagagcc agcagatcca
    18541 tatacattgc tttggtgtat ttggagcgac gaaacagcaa cggcagcgcc ttcagcgtgc
    18601 ctttaatcgc tttgccatta ttgccgacga taaacgaccc aatccctgca ccggcaataa
    18661 tcaccagttc agcgggttga tagagtgctc caaggcttcc accggtcatc aaataaccgc
    18721 cgaaaactgt accgagaaca accaggtaac ctaataagat aagcacgaca tcatccttcc
    18781 actgttgacc atgacaggat gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa
    18841 aagcagcggt acgtcgttac cgctgctgga atgttgcgcc tcaccgtatc agttaaacag
    18901 cctgtactct ctgttcatcc agcagttgtg ggataatatc ggcaggattc tgggaaagtt
    18961 tacgtctttt tactgcccgg gatggcggtt gacataagct gcaggcaaag ctgccaacag
    19021 gctggtgagc gtgggtaata aaattgccgc cgcagcagtt gcagctggaa agttgcagta
    19081 atccactttc aacaaaccgc accaatgtcc aggcacgggt taatgccagc agtggtcctt
    19141 cttctgcttg tgggcactgt tcaaggtata aacggtaggc tttgatcacc gcatcgacgc
    19201 cattacacaa accggttttc agtaaaaaca gccatgcatt acagaacatc gaagcatgaa
    19261 cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg
    19321 ggcttccgcg cagttcttta taaagtttta tcaggcgtcc gcgacttaaa tgtgtttcgc
    19381 tttccagcat ctgcaaacga gcgcccaggg tgatcaattc cattgccagc tgaatatccc
    19441 gcgcttcctg aacaatgctt ttttcactca tgatcaggcc cttttcttgc gcagcgcttc
    19501 ttcaggctga ttaacatcat tcagcaagcg tgttgagagc atgatgccgg tatgaatttg
    19561 ctggagatcg tcaacgcggg aatcttgcgt caactgagta atcgtctggt ggctgtcaaa
    19621 acggaagtga caaaccagtt gattggtttc tgccagctta accatttgcg gaagagtcag
    19681 tgccgctaac gttgtcgcca tttcttcatt tatgccgaga cgaaacatag cggacgcttt
    19741 gtcctgaaca atcaaacgct gtgcaagtag taaatatgac aagttgatgt cataaatgtg
    19801 tttcagcaac tcggaggtat gcattattcc cacccagaat aaccaacttt atttttatgc
    19861 ggtttcaccg caccccgtga tgtcgccggg aagccccggt aaaaaataat tagcattaga
    19921 atagttgcga taagctgcaa taagcagaac cacctttttg gtttaatatg tccttacaaa
    19981 tagaaatggg tctttacact tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc
    20041 actacacgca catacaacgg aggggggctg cgattt
//
LOCUS       DAPHJE010000083        19899 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPHJE010000083 DAPHJE010000000
VERSION     DAPHJE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.083"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            383..589
                     /locus_tag="RH222_003046"
     CDS             383..589
                     /locus_tag="RH222_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798033.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1150..1689
                     /locus_tag="RH222_003047"
     CDS             1150..1689
                     /locus_tag="RH222_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDV3798034.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATIRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            1698..3797
                     /locus_tag="RH222_003048"
     CDS             1698..3797
                     /locus_tag="RH222_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV3798035.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELMYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            3794..4006
                     /locus_tag="RH222_003049"
     CDS             3794..4006
                     /locus_tag="RH222_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798036.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4006..5514
                     /locus_tag="RH222_003050"
     CDS             4006..5514
                     /locus_tag="RH222_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV3798037.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWNEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRNLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5459..7486
                     /locus_tag="RH222_003051"
     CDS             5459..7486
                     /locus_tag="RH222_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDV3798038.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDRVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFIHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7573..7896
                     /locus_tag="RH222_003052"
     CDS             7573..7896
                     /locus_tag="RH222_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDV3798039.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7889..8164
                     /locus_tag="RH222_003053"
     CDS             7889..8164
                     /locus_tag="RH222_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HDV3798040.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8176..8754
                     /locus_tag="RH222_003054"
     CDS             8176..8754
                     /locus_tag="RH222_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3798041.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8751..9152
                     /locus_tag="RH222_003055"
     CDS             8751..9152
                     /locus_tag="RH222_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3798042.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9163..9906
                     /locus_tag="RH222_003056"
     CDS             9163..9906
                     /locus_tag="RH222_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3798043.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFVNPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            9967..10353
                     /locus_tag="RH222_003057"
     CDS             9967..10353
                     /locus_tag="RH222_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HDV3798044.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGTGAEM
                     VLSLSGMGAIENAGDLEHEVLTPEKS"
     gene            10362..10691
                     /locus_tag="RH222_003058"
     CDS             10362..10691
                     /locus_tag="RH222_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HDV3798045.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10663..13728
                     /locus_tag="RH222_003059"
     CDS             10663..13728
                     /locus_tag="RH222_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV3798046.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATEKMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNDLKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13728..14057
                     /locus_tag="RH222_003060"
     CDS             13728..14057
                     /locus_tag="RH222_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3798047.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            14067..14765
                     /locus_tag="RH222_003061"
     CDS             14067..14765
                     /locus_tag="RH222_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDV3798048.1"
                     /translation="MQDIPQETHHETTCLTQSAQVVLWEIDLTVVGGERYFFCNEQND
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14771..15514
                     /locus_tag="RH222_003062"
     CDS             14771..15514
                     /locus_tag="RH222_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3798049.1"
                     /translation="MTETESAILAHARRCASAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            15451..16059
                     /locus_tag="RH222_003063"
     CDS             15451..16059
                     /locus_tag="RH222_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDV3798050.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGSEAIRALAMQIPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQVVLGAALIAVS
                     WWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16120..19533
                     /locus_tag="RH222_003064"
     CDS             16120..19533
                     /locus_tag="RH222_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDV3798051.1"
                     /translation="MGKGSSKGHTPRETKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVQEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGNYRLTVRAVNARGQQGDPASVSFRIAAPAVPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVDLTEDNASRLDEFSKEWQDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPAITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            19604..>19899
                     /locus_tag="RH222_003065"
     CDS             19604..>19899
                     /locus_tag="RH222_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV3798052.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSV"
BASE COUNT         4789 a         4667 c         6007 g         4436 t
ORIGIN      
        1 ttagctcaga ataaaataat tcacagttta aatctttacg cacttgatcg aatatttctt
       61 taaaaatggc agcctgagcc attggtaaac cttccatgtg atacgatggc gcgtagttag
      121 cattatcgtg tttattgttt caatctggtc tgacctcttt gtgttttgtt gatgatttat
      181 gtcaaatatt aagcctgttt ttgatgaata gtgttgattg cgtaacaaaa ttgagccttg
      241 ctggcatcca ggagggatat gcaaccgaca gatgtatgta aggtcgatgt actcaaactt
      301 tcatactttt cctcttttat gcagtaagat ttgaagtaat attttaaccg ctagatgacg
      361 agcaaacgca tggagcgaca aaatgaataa agaacaatct gctgatgaac tctcgttgga
      421 tctgattcgt gtaaaaaata tgcttaatag caccatttct atgagttacc cggatgttgt
      481 aattgcatgt atagaacatc aggtgtctct ggaagcattc agggcaattg aggcagcgtt
      541 ggtgaagcac gataagaatt cgaaggatta ttccctggtg gttgactgag caccataact
      601 gctaatcatt caaactattt cacctgtgac agagtcaata tcgcattctg tcactgtcag
      661 gctaatacag agctgcaatt caactactgc aatgtcctcg taattaggtg aatttacaat
      721 atcgtcctgt tcggatgccg gttgcattgc tgaagatgag gcatttatgg ttcgcatatt
      781 ttcccctcat gctcgtcagt cctgtgcgta ggaagaaaca ggacactcac actaatttgt
      841 gtgggcatgc tgtgatgtcc ttctgaatta ttcctatgcc attatgtaaa gcgctgtatc
      901 agatgctcat cacggctgtc aggctgtcag gctgtcgggt cctcccggtg ggggcccctg
      961 ccacggggcg ggagcgtcgc ggaaaaaggc tagtttttgc atttccatgg cggcggcagc
     1021 atgtttggta atttattgat aattaaaagt tatttctctt ttcacctgta caatattttt
     1081 ttctccctgt cattagacca gtttgcaatt aattgaaata tataaataaa catgattttc
     1141 acctgccaga tggagttgct tatgtcaaat gtgagcggga tcggtgatgc ttattactgg
     1201 agtgttttta aaatcgctga ggcctttggg cttcaccggg acacagtaaa aaaacggctc
     1261 ctcgcggcca acactcctgt ggctgcgact atcaggggga accccgttta cgccctgcag
     1321 catgtcgggc cagccctgtt tagtgtgaag catgaggcag cagactctgt tcatgatcca
     1381 tcccgtatgg agccgaaaga gagaaaggac tggtaccaat ctgaaaatga aaggatcaag
     1441 ctggaaaagg agcagcgaaa actcatcccc gttgatgaag tagtcatcgt ctattcgtcc
     1501 atgagaaagg ctgtcgtcca ggttctggag acaattccgg atgttcttga acgcgattgc
     1561 gccctgactc ctcaggccgt cggcgttgta cagcaggcca ttgatgacct gcgatacact
     1621 cttcaggaaa aatcctacga ggcttgtgct gctgaattaa ttcctgatga ggaaggagag
     1681 agtctctagg aggaataatg ggtttttcat cagcccgaaa tttgggaagg gacatatcgg
     1741 caggattttc cccaccacgt cgcatgccga tttcggaggc tgttaaaaaa ttcatgcgtg
     1801 ttcccaaggg ggctggtaac tcggtgccat gggatcctga actgacaccc tacatcattg
     1861 agcccatgaa ctgcctggca tcgcgtgaat acgatgcggt gatttttgtt ggtcctgcgc
     1921 gaacagggaa gaccattggt ctgatcgatg gatggattgt ctataccatc gtttgcgatc
     1981 cttcggacat gctcgttgtg cagatgaccg aagataaggc ccgcgagcat tctaaaaagc
     2041 gcctcgacag aacgttcaga agcagtgcgg cggtaaagaa aagaatgagt ccacgtcgta
     2101 acgacaataa tgtccatgat aagacgttca gggatggctc gttccttaaa attggttggc
     2161 cctcggtcaa cattatgtcg tcgtcggatt accggtttgt cgccttaacc gattacgacc
     2221 gttttccgga gaatatcgac agcgagggtg atggtttctc cctggcctca aaacgtacca
     2281 ccacatttat gtccgccggg atgactctgg tggagagctc gccgggacgt gacatctgcg
     2341 acagcaaatg gcgacgtaag tcgcctcatg aagcgccacc gacgactggt attctttccc
     2401 tttacaatcg tggtgaccgc cgccgctggt actggtcatg tccgcactgc ggtgaatatt
     2461 ttcagccagc catggatgcc atgaccggct accgtaatga accggatccc tttaaagcca
     2521 gtgaggcggc gtatctactt tgcccgcact gcagcggcat tatcactgcg gagaaaaagc
     2581 gtgagctcaa tagtgcagga gtctggttgc gtgaaggtca ggtcattgat cgtaacggca
     2641 acgtttccgg tgaaccgcgc cgctcccgta tcgccagttt ctggatggaa gggccagctg
     2701 ctgcgtatca gacctgggcg caactggttt acaaattact gactgcagaa caggagtatg
     2761 aagcgacagg aagcgaagaa acactcaggg cggttatcaa caccgactgg ggattgcctt
     2821 atcttcctcg tgccagcatg gagcaacgaa aaagtgaact gcttgagcag cgggcagagc
     2881 cagttccttc ccgcagtgtg ccggatggcg ttaatttcct tgtggcgaca gtggatgtgc
     2941 aggcgggacg tcatcgccgt tttgtggttc aggtaacggg ctatggcagt cgtggcgaac
     3001 gctggattat tgatcgttac aacatcacgc agtcattgcg cagtgactgc gacggggaga
     3061 gccagcgaat tgatccggcc agctatccgg aagactggga tgtcctgctg acggatgttt
     3121 ttcataaaag ctggccgctg gcctccgatc cttctcaaca aatgcgactg atggcaatgg
     3181 cggtggactc cggcggtgaa gacggggtca ctgataatgc ctataaattc tggcgtcgtt
     3241 gccgtcgtga tggccttggt aaacgtattt acctgtttaa gggtgacagc atccggcgcg
     3301 caaaactgat cagccgtaca ttccctgata acaccggacg aacgggccgc cgggcgcagg
     3361 ccgcaggtga tgtgccgctc tggcttcttc agacggatgc cctgaaagac cgggtgaata
     3421 acgcgttatg gcgtgactcg ccaggtccag gctatgtgca tttccctgac tggctgggga
     3481 gctggtttta cgacgaactg atgtatgaag agcggagcag tgacgggaaa tggagtaagc
     3541 cgggtcgcgg tgccaacgaa gcttttgacc tgatggtgta tgccgaggct ctggtcattc
     3601 tgcatggata cgaaaagatc cgctggccgg atgcaccgga gtgggcgagc cgggagacct
     3661 ggctggagtg tgtccaggac agtaccgaac cgtcatcctc accggaaccg gtatccacgc
     3721 ctgttaaaaa acaaaaacgg aagaaaacag taactgacga tgttaacccc tggctgactt
     3781 ccggaggatg gttatgaacc agaatgatat cgaagccatg attcagcgtt atacggaagc
     3841 tgaaatggcg gtgctggacg gaaaatccgt cacctttaat ggtcagcaga tgaccatgga
     3901 aaacttatct gagatccggc agggacggca ggagtgggag cgccgccttg cggctctgat
     3961 tacacgacga cgggggcatc ccgggtaccg gctggcgagg ttctgatggc aattcttgat
     4021 gatgtgattg gcgttttttc accaggatgg aaagcggcaa ggctgcgttc ccgtgcggtg
     4081 atccaggctt atgaggccgt aaaaacgacg cggacacaca aagcccgacg ggagaaccga
     4141 actgccgacc agttaagcca gtacggggcc gtgtcgttac gtgagcaggc ccgttacctt
     4201 gataacaacc acgatctggt tattggtgta tttgacaagc tggaagaacg ggtggtgggg
     4261 aaaaacggga ttattgtcga gccacatccg gtattacgca atggggccat tgcccgtgac
     4321 ctggctgcgg agattcgcac ccgatggaat gaatggtctg tcagcccgga agtcaccggg
     4381 cagtttaccc gtccgatgct ggaacgtctg atgcttcgta cctggctgcg cgatggtgag
     4441 gtgtttgccc agatggtttc cgggcgcata aacagcctgg cgccttctgc cggtgttcat
     4501 ttctggctgg aggcgctcga gccggacttt attcccatga ccagtgatga gagcaacagg
     4561 ctgaatcagg gcgtgtttgt tgatgactgg gggcgtcccg aaaaatatct ggtgtataaa
     4621 agccgtcccg tatccggacg gcagatggaa accaaagaag tggatgcaga gcgaatgctg
     4681 catcttaaat ttgttcgccg tctgcaccag atgcgcggga cgtctttgtt gtccggtgtg
     4741 ctgatccgcc tcagtgccct gaaagaatat gaagattctg agctgactgc agcaaggatc
     4801 gccgctgctc tggggatgta catccggaaa ggcgacgggc agagctatga accggatggt
     4861 aatggcagaa aggagaatga acgtgagctt accattcagc cgggcattat ttacgacgat
     4921 ctgaaacccg gcgaagaaat cggaatggtg aagtcggatc gccccaatcc taaccttgaa
     4981 acttttcgta atggtcagtt gcgtgccgtg gcggcgggca gtcgtctgag tttttccagt
     5041 acagcgcgca actataacgg cacttacagc gcccagcgtc aggagctggt tgaatccact
     5101 gatggctacc tgatcctgca ggactggttt attggtgccg tcacccgccc gatgtatcgt
     5161 gcatggctga aacaggctgt ggcatccggt gttatcaggc taccccgcga tcttgaccgt
     5221 tcttcactgt ataccgcggt gtattccgga ccagtgatgc cgtggattga ccctgttaag
     5281 gaggctgagg cctggaaaat ccagattcgt ggtggagcgg cgacagaatc agactgggta
     5341 cgtgctggtg gtcgtaatcc ggatgatgtc aaacgtcggc gcaaggccga aattgatgaa
     5401 aaccgcaatc tggatctggt atttgatacc gatccggcca gtgataaagg aggcagtagt
     5461 gccgcaacga aacgacagga gccgcagcac accgacgacc agtccgaaga ataattcctg
     5521 gttcaggatg caggctggtc accagagtga cgcggatatt tatatttatg acgagattgg
     5581 tttctggggt gttacagcga agcagtttat cagtgatctg aatgcactgg gcgatatcac
     5641 ccacattaat ctccatatta attcaccggg tggcgatgtc tttgaaggca tcgccatttt
     5701 taatgcgctg aaaacacatg gtgcgtccat taccgtttat gtcgacggtg tggcggcgtc
     5761 aatggcgtcg gtcattgcga tggtaggaaa cccggtcatt atgccggaaa acaccttcat
     5821 gatgattcat aaaccatttg gctttacggg cggtgatgcg gaggacatgc gcacctatgc
     5881 cgacctgctc gatagagttg aggcggttct gttacccgct tatgcacaga aaaccgggaa
     5941 aaccaccgat gaaattgctg ccatgctggc ggatgagacc tggatgtccg gtgccgaatg
     6001 tctggcacag ggatttgctg atcaggtgac accagccgtt aaggcaatgg catgtattca
     6061 gtcaaaacgt acagaggaat ttaaaaagat gccggaatcc attcgaaata tgattactcc
     6121 gccacgcaac agtgctccac gcgtacagga tgatggacct gcagcctccc ggacgccagt
     6181 gcaggcagca gcacccgtgg tggatgaaaa cagcatccgt gcgcaggtcc tggcagagca
     6241 aaaagcgcgt gtaaacggta ttaatgatct gtttgccatg tttggcgggc gttatcagtc
     6301 gttgcaggct cagtgtcttg ccgatcctga atgttcgctg gagcaggccc gcgaaaagct
     6361 gttgaacgag atggggcgcg agtccacgcc atccaataaa aataccccgg ctcatattta
     6421 tgccggtaac ggtaattttg tgggggacgg gatccgccag gcgctgatgg cgcgcgccgg
     6481 gtttgaaaaa actgaacgtg ataatgtcta caacgggatg accctgcgtg aatatgcccg
     6541 tatgtcactg actgaacgtg gtattggtgt ttccagttat aacccgatgc agatggtcgg
     6601 tgcggcgttc atacacagta cgtctgactt cggtaatatt ctgctggatg ttgcgaacaa
     6661 agccattctg cagggctggg aagatgcccc tgaaacctat gaacagtgga cgcggaaagg
     6721 tcagttgtct gattttaaaa ttgcccatcg tgtgggtatg gggggcttca gtgctctgcg
     6781 tcaggtgcgt gaaggggcag aatataaata cgtcaccacc ggagataaac aggccactat
     6841 tgcactggcg acctatggcg agctgttcag tatcacccgt caggccatta tcaatgatga
     6901 tctgaatatg ctgaccgatg tcccgatgaa gctgggccgt gcggcgaaat ccactattgc
     6961 cgatctggtt tatgccattc tgacgtctaa cccgaaaatc tccacagata atgtaagtct
     7021 gttcgataaa gcgaaacatg caaacgtact ggagagcgcg gcaatggacg tggcatcgct
     7081 ggataaagcc cgccagttga tgcgcgttca gaaagagggt gagcgtcatc tgaatattcg
     7141 tcctgcgttc gtactggtac cgacggcgat ggagtctgtt gctaaccagg tcattcgctc
     7201 ctcaagtgtc aagggggctg acattaacgc cggtattatt aacccggtga aagattttgc
     7261 gaccgttatt gcagagcctc gtcttgatga taacagccag accaccttct acctggctgc
     7321 gtccaaaggc tccgatacga ttgaagtggc ttatctcaac ggtgtggata cgccatatat
     7381 tgatcagatg gagggcttca gtgtggatgg cgtgacaacg aaagtgcgta ttgacgctgg
     7441 tgttgcgcca gttgatcacc gcggcctggt gaaatgtacg gcgtaaacgt cgcagacaac
     7501 aactctgatg gcccgtaagg gctttttttg tacctgaaat cagcccctga acggggctgt
     7561 gcggagacag ttatggcaaa gaattttgta gaagaaggaa aaacggtggc gattgttgca
     7621 agtgcagcca tcagcagcgg agatctggtg caggtgggtg atgtttttgc ggtggcgctg
     7681 accgatattc cacagggaga aacaggcgac ggcataaccg aaggtgtgtt tatgctgcct
     7741 aagctgaaaa cggatgacat gaaaacgggt aagaaggttt atctgaagtc aggaaaagtt
     7801 cagctgacta acagcggctc tgatccgctg gtcggggttg tctgggcaga tgccggaacc
     7861 agtgcagaag aagtgccggt aaaactcaat gtctgatccc ttttcccggc tggcagcgcg
     7921 tatggatgcg atcacggtca gaaagatggg aaagacagcc tcgattaatg atgtcgatat
     7981 gactgtgatc ccgggagaaa cactggcaga gctgaatgct ctgtccggac ctgcggtctc
     8041 tctggtggtg ttttcttcgg gataccgccc acggcgcggg gatcgcgttg tttatgacgg
     8101 acaacaatgg acggtcacac ggcatgaacg ttttaacggt aagccaatga tctttattga
     8161 gtaatgaggt gtgggatgaa ggggcttgag aatgccatcc gcaatctgaa cagccttgat
     8221 acccgtatgg tgccacaggc cagcgcatgg gcgataaacc gtgtggcaca gaaagcggtc
     8281 tcggttgcca cccggcaggt tgccgggaat accgttgcgg gagataacca ggtgaaaggg
     8341 atccccctga aactggtacg tcagcgtgtc cgggtgttta aagccagtcc gtcaggaaaa
     8401 atgacggcca ggatccgcgt taaccggggc aatctgcccg ccattaagct ggggacagcc
     8461 cgggtcagac tggcccggcg tggtggaaaa ctgcagtacc gtggcagtgt gctgaaggtg
     8521 ggtaaatatc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg ctggcatgtg
     8581 atgcgacgta ttgatggcaa aaatcgttac cccattgatg tggtgaaaat cccgctgtcc
     8641 ggaccgctga cacaggcatt tgaagatgcc cgcgaccaca tcattgctgc ggaaatgccg
     8701 aaacagctgg ggtatgcact gaaacaacaa ctgaggttat ggctgacccg atgaaccgac
     8761 atacacaaat ccgccaggtc gtactggcac gccttcggga acagtgtgga gacagcgcca
     8821 cgttttttga cgggcttccg gcatttgttg atgcgcagga actgcctgcc gtggcggtgt
     8881 ggctgagtga tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc
     8941 tgcatattgc tgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg
     9001 agagcaccat tttcccggcc ctgaatgatg taccagcact ttccggactc atcgacaccc
     9061 tgatcccact cggttttaac tatcaacgtg ataatgagat ggccacctgg gcgatggcgg
     9121 aaatcacgta ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca
     9181 ctggcaaaaa cgaaaggtgc gggaacgacg ttctggatgt ataccggcaa gggcgatgcg
     9241 tttgtgaacc ctttatcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa
     9301 cctggcgaaa tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac
     9361 tggaaaacga caacccaggg gcagaaatca gtcggtgata cttcggcgac gctggcctgg
     9421 cgtccggggg acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc
     9481 gcgtttcgta tcaaatatcc caacggtact gttgatgttt tccgtggctg gctgagttca
     9541 ctgggtaaaa ccattgcttc aaaagacgtg atgacccgca cagtgaaaat cagcggtgtg
     9601 gggcgtccgt atctggcaga ggaaggcact gaaaccgtgg gcgttaccgg gctgacggtg
     9661 gcaccggcat ctgccagtgt aaaagtggga gcaaccacca cgctgacctt tacagtaaaa
     9721 cctgacggag ccagtgacaa agcgatcagt gtgcattcga cagatccaca gactgccacg
     9781 gtgaccctga acgggcttgt ggccacggtg aaaggcgtga agcagggcag tgtcagcatt
     9841 gtgggcatga cttctgacgg cgattttgtg gcagtggcta cggtggctgt cagcgccgca
     9901 ggttaacagg acgatactca tcatttgccc cggttatccg gggctttttt gcaggtggag
     9961 aacatgatgt ttctgaaaca gggcacgttt aattatgaaa agcagtccgt ggtgctcagt
    10021 gagctgtccg ggctgcagag aattgaatat ctggcgtttg ttcagcagcg aacggcaaag
    10081 tttgatgccg aagagggaga actgccggag gctgaacgac agattgcttt tctgcggatg
    10141 gggatggata tcaatgcctg gctggtttcc cgctcactgt ggaatgcgga acagtctcag
    10201 gatgttgaga cgctttgcgc atccgttatt acaacatggt cgtatgatgc cctgggaacg
    10261 ggggcggaga tggttctgtc gctgagcggt atgggagcca ttgagaatgc cggggatttg
    10321 gagcatgagg tgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc
    10381 ttgcccggga gttcaggcgg gcagactggc ggcggatgct gtcggaaatg tcggccactg
    10441 agcttggtga gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg
    10501 cgcagtttgc ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agtgatgctg
    10561 cggtggctga tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag
    10621 aactgatgca tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac
    10681 ctggtcattg atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa
    10741 aaccttctga atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt
    10801 atggagcgtc agactcaggc cgcccggcag acaacgcagg cggcttcttc ggctgcaaca
    10861 gccgcatccg tccatgcgca gacggtggag aagaacgcac aggctcatga acgcatggcc
    10921 cgcgaggtgg agaaaacccg ccagcgcatg gaggcgctga gccagaaaat gcgcgaggaa
    10981 caggcgcagg ccatggctct ggcggaggct caggataaag cggctgctgc gttttatcgt
    11041 cagattgaca gtgtgaaaca ggccagtgcg gggctgcagg aattacagcg tattcagcag
    11101 cagatccgac aggccagaaa cagtggcggg attggtcagc aggattatct ggcgctgatt
    11161 tctgaggtta ctgcgaaaac ccgtgttctt acacaggctg aggaagaggc tacccgacag
    11221 aaagtggcgt ttatccgtca gcttaaagag caggcaaccc gccagaatct ttcatcttct
    11281 gagttgcttc gtgccaaggc agcccagctg ggggtaagca gtgctgcaga agtgtatatc
    11341 cgcaaaatgg agcaggcagg aaaagccacg cattcgctgg gtctgaaaag tgcagcagcc
    11401 cgccaggaga taggcgttct gataggtgaa ctggcccgcg gcaatttagg ggcgctgagg
    11461 ggatccggga taacgctggc taaccgtgcc ggatggatag acacactgat gtcaccgaaa
    11521 ggcatgatgc ttggcggggt tattggcggt attgccgcgg ccgtctatgg tctgggtaaa
    11581 gcctggtatg acggtcagaa ggagggggaa gaatttaacc gccagttgtc gctgacgggg
    11641 cattatgccg gagtcactgc cgggcagctg tggacgctca gtcgtgctat ttccgggaat
    11701 ggtatcacgc aacatgctgc agccggtgcg ctggctcagg tggtggggag tggtgcattt
    11761 cgtggaaacg atatcggtat ggtggcgaga gctgccgcac agatggagcg atcggttggc
    11821 cagtcggtca gcgataccat aaatcagttt aagcggctga aggatgatcc tgtaaatgcc
    11881 gcgaaggctc tggacaatga gctgcatttt cttactgcca ctcagcttga gcagatacgc
    11941 gtccttgggg agcaggggcg gtccagtgat gctgcacgga tagccatgtc tgcactggca
    12001 gaggaaaccg gtcggcgtac tgcggatatt gataataacc tcaatgcgct tggcagtacg
    12061 ctgaagtatc tgtctgattt gtggagtcgt ttctgggatg cggccatgaa tattggtcgt
    12121 gaagactcgc tggatgaaca gattgccgct ttacaggaga aagtgtcgcg ggcgaaaaga
    12181 ctcccctgga cggcatcatc ttctcaggtt gaatacgatc agcagcgtct taacgatctt
    12241 caggagaaaa aacgccagaa ggatttgcag gatgcaaaag agcaggcaga gcggaattat
    12301 caggagcaac agaaacgacg taatgctgaa aatgctgctc tgaaccggat gaatgaaacg
    12361 gaagcagcac gacatcagcg tgaaattgcg cgtattaatt ccatgcagta cgccgatcag
    12421 gctgtcaggg atgcagcgat acaacgtgaa aatgaacgtt acgagaaagc cctggcatcc
    12481 ggtaagaaaa aaacacgcga aacccgtaat gatgaggcca cccggttatt gctgcagtac
    12541 agtcagcaac aggcacaggt ggaaggacag attgctgctg caagacagtc agcaggcatt
    12601 gccactgaaa agatgacaga agcgcataaa cagcttctgg ctctgcagca gcgcatcagc
    12661 gatctggacg ggaaaaaact gacggcagat gaaaagagtg tgctggcccg taaagatgaa
    12721 ctgattcagg cactgacgct gctggatgta aaacagcagg agcttcagaa acagacggca
    12781 ctcaacgatc tgaagaaaaa aacaattcag ctgaccagtc aactggctga agaagagcgc
    12841 gctcagcgcc agcaacatga cctggatatc gccacggtgg gtatgggtga tcagcagcgg
    12901 cagcgatatc aggtacaact gagtcttcgc cagaaatacc agcaacagct ggagcagttg
    12961 aggcgggata gtgagcagaa agggacatat aacacggatg actacagaaa ggccgagcag
    13021 gcgctgacgg agagcctgaa ccgacaactg aatgagaatc gccgttactg gcaacagctt
    13081 gaagttgtgc agggtaactg gaaaaacgga gtcctgcgtg catttcagga ttttaccgtg
    13141 gatgcagata atacggcagg aacagcagaa caggtgttct cgtcagcctt cagcaacatg
    13201 ggaaatggcc tggcaacttt tgtcactacc ggcaaactca acttcaaatc cttcacctct
    13261 tcggtgctgt cagatatggc gaaaatcctg gcgcaggcaa ccatgatgaa atcgataaaa
    13321 gggattggca gtgtactggg atttgatctc agcagccttt ccctgaatgc caatgggggg
    13381 atttatcagt ctgctgattt gagtcgttac agtggcacgg tggttaaccg tccgacgttt
    13441 tttgcttttg caaaaggcgc gggtgtgatg ggggaagctg gacctgaagc cattctgcca
    13501 ctgcgtcgtg gtgctgacgg taagctgggg gttgtggcgg atattggtgg ttcaggtatg
    13561 gcgatgtttg ccccgcagta caacatcgag atcaataacg atggcacgaa cgggcagata
    13621 ggtccggctg ccctgaaggt ggtttatgac cttgggaaaa aagcggcagc ggactttatg
    13681 caacagcagg cccgtgatgg tggtcggtta agtggagcat atcggtaatg gagacgtttc
    13741 actggaaagt gcgcccggat atgaatgtgg tatcagagcc gaaagtggtg acagtgaagc
    13801 tgggcgatgg ttatgaacag cgtcgtgcgg cgggactgaa taaccagttg tcgatttaca
    13861 gcgtgacgat acgtgttcgt aaatgtgaac acccatcttt aaaagccttt ctggaacggc
    13921 acggtggcgt ccgcgcattt cagtggacgc caccttatga ctggaagccg atcagggtgg
    13981 tttgtcgtaa atggtcggca agcgtggggg cgttgtgggt aaccataacg gcagattttg
    14041 aacaggtcgt ggcataggag tctctgatgc aggatattcc acaggaaaca catcatgaga
    14101 cgacatgcct cactcagtca gcccaggtgg tgctctggga aatcgatctg acagtggtcg
    14161 gtggagaacg ttattttttc tgtaatgagc agaacgataa aggtgagccg gtcacctggc
    14221 aggggcgaca gtatcaggcg tatcccattc aggggacggg atttgaactg aacggcaaag
    14281 gcagtgctgc ccgtccgaca ctgacggttt ctaacctgca cggcatggtc accgggatgg
    14341 cggaagacct gcagagtctg gtcggaggaa cggtggtccg gcgtaaggtt tacgcccgtt
    14401 ttctggatgc ggtgaacttc gtcaacggaa acagtgacgc cgatccggag caggaggtga
    14461 tcagccgctg gcgcattgag cagtgcagcg aactgagcgc ggtgagtgcc tcttttgtac
    14521 tgtccacgcc gacggaaacg gacggcgctg tttttccggg acgtatcatg ctggccaaca
    14581 cctgcacctg gacctatcgc ggcgatgagt gcggttatca cggtccggcg gtcgcggatg
    14641 aatatgacca gccaacgtcc gatatcacga aggataaatg cagcaaatgc ctgagcggtt
    14701 gtaagttccg caataacgtc ggcaactttg gcggcttcct ttccattaac aaactttcgc
    14761 agtgaatccc atgacagaga cagaatcagc gattctggcg cacgcccggc gatgtgcgtc
    14821 agcagagtcg tgcggcttcg tggtgagaac accggagggg gaaagatatt ttccctgcgt
    14881 gaatatctcc ggtgagccgg aggcgtattt ccggatgtcg ccggaggact ggctgcgggc
    14941 agagatgcag ggtgagattg tggcgctggt ccacagccac cccggtggtc tgccctggct
    15001 gagtgagacc gaccggcggc tgcaggtgca gagtgatttg ccgtggtggc tggtttgccg
    15061 gggggcgatt cacaagttcc gctgtgtgcc acatctttcc gggcggcgct ttgagcacgg
    15121 ggtgacggac tgttacacgc tgttccggga tgcttaccat ctggcgggga ttgagatgcc
    15181 ggattttcat cgcgaggatg actggtggcg tcacggtcag aatctctatc tggataatct
    15241 ggaggccaca gggttgtatc aggtgccgtt gtcagcggcg cagccgggcg atgtgctgct
    15301 gtgctgtttt ggttcatcgg tgccgaatca tgccgccatt tactgtggtg acagcgagct
    15361 gctgcaccat attcctgaac aactgagcaa acgagagagg tacaccgaca aatggcagcg
    15421 acgcacacac tccctctggc gtcaccgggc atggcacgca tctgccttta cggggattta
    15481 caacgatttg gccgccgcat cgaccttcga gtgaaaacgg ggtccgaagc catccgggcg
    15541 ctggccatgc agatcccggc gtttcgtcag aaactgagcg acggctggta ccaggtacgc
    15601 attgccgggc gtgatgcagg tgaaaccgaa ttgtctgccc gtctgaatga gccgctggca
    15661 aatggtgccg tgatccacat cgtgccgcgt ctggcgggag ctaaaagtgg cggtgtgttt
    15721 caggtggtgc tgggggcggc gctgattgcg gtgtcatggt ggaaccctgt gggctggctg
    15781 ggtgccgcgg ctgtatcggg catgtatgcg gcaggggcca gtatgatcct gggcggagtg
    15841 gcgcagatgc tggcaccgaa agccaggacg cccacggcag caagtacaga taacggcaaa
    15901 cagaacacct atttctcctc actggataac atggttgccc agggcaatgt tctgcccgtt
    15961 ctgtacggtg aaatgcgcgt ggggtcgcgg gtggtctctc aggagatcag cacggcagac
    16021 gaaggggacg gtggtcaggt tgtggtgatt ggtcgttgat gaaaaacgtt ttatgtgaaa
    16081 ccgcttccgg gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag
    16141 gggcatactc cgcgcgaaac gaaggacaac ctgaagtcca cgcagttgct gagtgtgatc
    16201 gatgccatca gcgaagggcc gattgaaggt ccggtggatg gattaaaaag cgtgctgctg
    16261 aacagtacgc cggtgctgga cagtgagggg aataccaaca tctccggcgt cacggtggtg
    16321 ttccgggccg gtgagcagga gcagacaccg ccggagggat ttgaatcctc cggctccgag
    16381 acggtgctgg gtacggaagt gaaatacgac acgccgatca cccggaccat cacgtcggca
    16441 aacattgacc gactgcgctt taccttcggt gtgcaggcac tggtggaaac cacctcaaag
    16501 ggggaccgga atccgtcgga agttcgcctg ctggttcaga tacagcgtaa tggtggctgg
    16561 gtgacggaaa aagacatcac cattaagggc aaaaccacct cgcagtatct ggcctcggtg
    16621 gtggtggata acctgccgcc gcgcccgttc agtatccgga tgcgcaggat gacgccggac
    16681 agcaccacag accagctgca gaacaaaacg ctctggtcgt catacaccga aatcatcgat
    16741 gtgaaacagt gctacccgaa tacggcactg gtcggcgtac aggtggattc ggagcagttc
    16801 ggcagccagc aggtgagccg taattatcat cttcgcgggc gcattctgca ggtgccgtcg
    16861 aactataacc cgcagacgcg gcaatacagc ggtatctggg acggaacgtt aaaaccggca
    16921 tacagcaata acatggcctg gtgtctgtgg gatatgctga cccatccacg ctacggcatg
    16981 gggaaacgtc ttggtgcggc ggatgtggac aaatgggcgc tgtatgtcat cggccagtac
    17041 tgcgaccagt cagtgccgga cggctttggc ggcacggagc cgcgcatcac ctgtaatgcg
    17101 tacctgacca cacagcgcaa ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc
    17161 tgtatgccgg tatggaacgg gcagacgctg acgttcgtgc aggaccgacc gtcggataag
    17221 gtgtggacct ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac
    17281 agcttcagcg ccctgaagga ccgccataat gccgttgagg tgaactggat tgacccggac
    17341 aacggctggg agacggcgac agagcttgtt gaagatacgc aggccattgc ccgttacggt
    17401 cgtaatgtca cgaagatgga tgcctttggc tgtaccagcc gggggcaggc acaccgcgcc
    17461 gggctgtggc tgattaaaac ggaactgctg gagacgcaga ccgtggattt cagcgtgggt
    17521 gctgaagggc ttcgccatgt accgggcgat gtcattgaaa tctgcgatga tgactatgcc
    17581 ggtatcagca ccggtggtcg cgtgctggcg gtgaacagcc agacccggac gctgacgctc
    17641 gaccgtgaaa tcacgctgcc atcctccggt accacgctga taagcctggt tgacggaagt
    17701 ggtaatccgg tcagcgtgga ggtccagtcc gtcaccgacg gacttaaggt gaaagtgaac
    17761 cgggttcctg acggcgttgc agaatacagt gtgtgggggc tgaagttgcc gacgctgcgc
    17821 cagcgcctgt tccgctgcgt gagtatccgt gagaacgacg acggcacgta tgccatcacc
    17881 gccgtgcagc atgtacagga aaaagaagcc atcgtggata acggggcgca ctttgacggc
    17941 gaccagagcg gcacggtgaa tggtgtcacg ccgccagcag tgcagcacct gactgccgaa
    18001 gttaccgcag acagcgggga gtatcaggta ctggcccgct gggatacgcc gaaggtggtg
    18061 aagggcgtga gtttcatgct tcgcctgacc gtggcagcgg atgacggcag tgagcggctg
    18121 gtcagcacag cccggacggc ggaaaccaca taccgcttca cacaactggc gctggggaac
    18181 tacaggctga cagtccgggc ggtaaatgcc cgggggcagc agggcgatcc ggcatcggta
    18241 tcgttccgga ttgccgcacc tgcagtgccg tcgcggattg agctgacgcc gggctatttt
    18301 cagataaccg ccacgccaca tcttgccgtt tatgacccga cggtacagtt tgagttctgg
    18361 ttctcggaaa cgcggattac cgatatcagg caggtagaaa ccacagcccg ctatcttggt
    18421 acggcgctgt actggatagc cgccagtatc aatatcaaac cgggccatga ttattacttt
    18481 tatatccgca gtgtgaacac cgttggcaaa tcggcattcg tggaggccgt cggtcgggcg
    18541 agcgatgatg cggaaggtta cctggatttt ttcaaaggcc agataaccga atcccatctc
    18601 ggcaaggagc tgctggaaaa agttgatctg acggaggata acgccagcag actggatgag
    18661 ttttcgaaag agtggcagga cgctaacgat aaatggaatg ccatgtgggg cgtcaaaatt
    18721 gagcagacca aagacggcaa acattatgtc gcgggtattg gcctcagcat ggaggacacg
    18781 gaggaaggca aactgagcca gtttctggtt gccgctaacc gtatcgcgtt tattgacccg
    18841 gcaaacggga atgaaacgcc gatgtttgtg gcgcagggca accagatatt catgaacgac
    18901 gtgttcctga agcgcctgac agcccccgcc attaccagcg gtggcagtcc tccggtattt
    18961 tccctgacat cagacggaaa gctgaccgct aaaaatgcgg atatcagtgg tagtgtgaat
    19021 gcgaactcag gaacgctcaa caatgtcacg attaaccaga actgtacgat taagggcatg
    19081 ctggaggcga cccaggtcag aggggatttc gttaaagctg tatcaaaagc cttcccgaaa
    19141 aaagtcggta cgtggggtaa cacggaaaca ccaaacggta cggttacagt caccatcagc
    19201 gatgatcata actttgaccg ccagattatt attccgccca ttatttttaa cggtatagcg
    19261 tatgacgatc cggggagcgg aaataacccg ggaggcacgc gatacacggg ttatggtttt
    19321 gaagttcgca aaaacggcgt attaatcgca tccagagaaa ctaaaggggc cattcccggt
    19381 agttacagtg cagttattga tatgcctagt ggtggtggta gcgtcactct ggagtttaag
    19441 attttccaga aaggcaatca gggggcaggc aatatcaccg actgtacggt gattgtgacc
    19501 aaaaaagcgg cttccggcat cagtattcgt tgaaatattt ataaccccaa taaatggcgt
    19561 caggaatgac gcctttttta ttgcagaaaa gcgagaggta attatgcgta aagtttgtgc
    19621 cgccattttg tccgcagcca tttgtctggc cgtatccggt gcgcctgcat gggcgtctga
    19681 acatcagtcc acgctgagcg cggggtatct tcatgcccgg accaacgttc ccggcagtga
    19741 tgatctgaac gggattaacg tgaaataccg ttatgagttt acggatacgc tggggctggt
    19801 gacgtcattc agctatgcag gagacaagaa tcgccagctg acccattaca gcgatacccg
    19861 ctggcatgaa gattccgttc gtaaccgctg gttcagcgt
//
LOCUS       DAPHJE010000084        19730 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPHJE010000084 DAPHJE010000000
VERSION     DAPHJE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.084"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(537..913)
                     /gene="rnpB"
                     /locus_tag="RH222_003066"
     ncRNA           complement(537..913)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RH222_003066"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(946..2091)
                     /gene="garK"
                     /locus_tag="RH222_003067"
     CDS             complement(946..2091)
                     /gene="garK"
                     /locus_tag="RH222_003067"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV3798053.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2188..3078)
                     /gene="garR"
                     /locus_tag="RH222_003068"
     CDS             complement(2188..3078)
                     /gene="garR"
                     /locus_tag="RH222_003068"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV3798054.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3108..3878)
                     /gene="garL"
                     /locus_tag="RH222_003069"
     CDS             complement(3108..3878)
                     /gene="garL"
                     /locus_tag="RH222_003069"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV3798055.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     IDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3894..5228)
                     /gene="garP"
                     /locus_tag="RH222_003070"
     CDS             complement(3894..5228)
                     /gene="garP"
                     /locus_tag="RH222_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV3798056.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWSVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5603..7174
                     /gene="garD"
                     /locus_tag="RH222_003071"
     CDS             5603..7174
                     /gene="garD"
                     /locus_tag="RH222_003071"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV3798057.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNIGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7323..7658
                     /gene="prlF"
                     /locus_tag="RH222_003072"
     CDS             7323..7658
                     /gene="prlF"
                     /locus_tag="RH222_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV3798058.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7658..8122
                     /gene="yhaV"
                     /locus_tag="RH222_003073"
     CDS             7658..8122
                     /gene="yhaV"
                     /locus_tag="RH222_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV3798059.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8177..8986)
                     /gene="agaR"
                     /locus_tag="RH222_003074"
     CDS             complement(8177..8986)
                     /gene="agaR"
                     /locus_tag="RH222_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV3798060.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPTDSLEGLRKAGVEVILVGE"
     gene            9235..10515
                     /gene="kbaZ"
                     /locus_tag="RH222_003075"
     CDS             9235..10515
                     /gene="kbaZ"
                     /locus_tag="RH222_003075"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV3798061.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10538..11011
                     /gene="agaV"
                     /locus_tag="RH222_003076"
     CDS             10538..11011
                     /gene="agaV"
                     /locus_tag="RH222_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV3798062.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11022..11801
                     /locus_tag="RH222_003077"
     CDS             11022..11801
                     /locus_tag="RH222_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV3798063.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11791..12669
                     /locus_tag="RH222_003078"
     CDS             11791..12669
                     /locus_tag="RH222_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV3798064.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12687..13166
                     /locus_tag="RH222_003079"
     CDS             12687..13166
                     /locus_tag="RH222_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3798065.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQVLEREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13163..14296
                     /gene="nagA"
                     /locus_tag="RH222_003080"
     CDS             13163..14296
                     /gene="nagA"
                     /locus_tag="RH222_003080"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV3798066.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEKVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14647..15801
                     /locus_tag="RH222_003081"
     CDS             14647..15801
                     /locus_tag="RH222_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV3798067.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15814..16674
                     /gene="kbaY"
                     /locus_tag="RH222_003082"
     CDS             15814..16674
                     /gene="kbaY"
                     /locus_tag="RH222_003082"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV3798068.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVIDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16841..17317
                     /gene="agaB"
                     /locus_tag="RH222_003083"
     CDS             16841..17317
                     /gene="agaB"
                     /locus_tag="RH222_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV3798069.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17356..18159
                     /gene="agaC"
                     /locus_tag="RH222_003084"
     CDS             17356..18159
                     /gene="agaC"
                     /locus_tag="RH222_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV3798070.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18149..18940
                     /gene="agaD"
                     /locus_tag="RH222_003085"
     CDS             18149..18940
                     /gene="agaD"
                     /locus_tag="RH222_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV3798071.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18941..19696
                     /locus_tag="RH222_003086"
     CDS             18941..19696
                     /locus_tag="RH222_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV3798072.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4777 a         5134 c         5031 g         4788 t
ORIGIN      
        1 atgattacga ccttttaaat gcaatataga cttaataact tcttacaaag tctattatta
       61 ataccgtaaa tattcatgct tcttaccctg tgcaccaaaa gcaaaaactt atcgatgcgg
      121 gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct acgcttatca ggcctacgcc
      181 atctctgcaa tatattgaat ttgcgtgctt ttgtaggcag gataaggcgt tcacgccgca
      241 tccggcatga ataaagcgca ctcaacaatc tgaaacccgc cggaacggtt tattacgtac
      301 atcaggtgaa actgaccgat aagccgcttt cttttgggta tagcgtcgtg gacagtcatt
      361 catctttctg cccctccaaa agcaaaaacc cgccggttta cgctggcggg ttttcgttaa
      421 atcaggtgaa actgaccgat aagccgcttt cttttgggta tagtgtcgtg gacagtcatt
      481 catctttctg cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg taaatcaggt
      541 gaaactgacc gataagccgg gttctgtcgt ggacagtcat tcatctaggc cagcaatcgc
      601 tcactggctc aagcagccta cccgggttca gtacgggccg taccttatga acccctattt
      661 ggccttgctc cgggtggagt ttaccgtgcc acggactgtt accagccgcg cggtgcgctc
      721 ttaccgcacc ctttcaccct tacctgatcc cgcttgcgcg ggccatcggc ggtttgctct
      781 ctgttgcact ggtcgtgggt ttccccccca ggcgttacct ggcaccctgc cctatggagc
      841 ccggactttc ctcccctccg cccgtctccc ccgaagagga cgacgacgaa gcggcgactg
      901 tctggtcagc ttcggcgcgc agtatagagg gtttgcgcgc ccttgtcacc ccgcgttgcg
      961 cattccaatc gccagtgtcg cggcgatatt acgtgaagca cggcagatat tgtcataagc
     1021 cccgcggaat gcttcgtcca acgtacctat gctggtcaat acgctgaaga ccgcatcaat
     1081 gccatgctga tgtacaacgc caacatcatc ggtcaggcta cccgcaatgc caatcaccgg
     1141 tttatggtac ttcttcgcca cgtttgcgac accaatcggt accttcccgt gaatactctg
     1201 gctgtcaata cgcccttcac cggtgatcac cagcgtacaa tcgtgaatat gttcctccag
     1261 attcagcgcc gtagtgacga tttcaatacc acttttcagt tccgcaccaa gaaacgccat
     1321 tagcgccgcg cccataccac ccgcagctcc tgcaccgggg acatctttca catcaacatg
     1381 cagcgctttt ttaatgacct cggcatagtg agagaggtta ttgtccagct caacaatcat
     1441 cgcttcactg gctccctttt gtgggccaaa gatgcgcgat gcgccgttat cgcccaccag
     1501 cggattggtg acatcacaag cgacgcgaat gacgcaatct tttaagcgcg gatcgaggcc
     1561 ggaaatatca atatcattca gagtattaag actaccgccg ccaaaaccaa tttcattgcc
     1621 gttggcgtcg cataatttcg cccccagcgc ctgtaccatg cctgcgccgc catcatttgt
     1681 agcgctaccg ccaatgccga taataatgtt tgtcgcgccg ctctccagcg cctgcaggat
     1741 taactcgcct gtgccgcgtg aagtggtcac gagtggatcg cgtttttccg caggtaccag
     1801 ctccagccca ctggccgccg ccatttcaat aaacgcggtt ttgccatcgc cggagatccc
     1861 ccaactggca ttcactttct cgcccagcgg ccctgtaacc caagcgtgac gttcagaacc
     1921 ctgggtggct gcaatcatcg cttccaccgt tccttcgcca ccgtcggcaa ccggaataga
     1981 aacgtactgt gcatcaggaa aaatttcccg aaatcctttt tctatcgcct gcgcaacctc
     2041 gctggcagat aaactttctt tataagagtc tggggcgatt acgattttca tacctatgcc
     2101 tgttaccaca tgacgccgga gggcgtttct cttattcggc ctggattcca ggcccggatt
     2161 gcaatacgcc atccgggcac gacgtcatta acgagtaact tcgactttcg ccagtttttc
     2221 gtagtagcac gccagggcgc tatgatccgc cgttcctaaa ccatctgctc gcagtgcctg
     2281 catcatctcc ataaccgcag ctgtgagcgg cagttgcgcg ccgacgccgt gggaagtatc
     2341 cagcgcattt gccagatcct taatatgcag atcaatacgg aagcccggct tgaagttgcg
     2401 gtccatcacc atcggcgctt tggcatccag cacggtactg cccgccagtc caccgcgaat
     2461 tgcctgataa accaggtccg ggttaacgcc cgctttggtt gccagcgtta gcgcttctga
     2521 catcgcggca atattcagcg ccacaatgac ctgatttgcc agtttggtga cgttacctgc
     2581 accgatttcc ccggtatgca ccacggaacc cgccatcgct ttcatcaaat catagtattt
     2641 gtcgaaaata gccttgtcgc cgcccaccat cactgacagc gtaccgtcga tggctttcgg
     2701 ttcaccgccg ctcaccggag catccagcat atcaatgcct ttcgctttca gcgcttcgct
     2761 gatttcacgg cttgccagcg gtgcgataga actcatatcg atcaataccg tacctggctt
     2821 cgcgccttca atgatgccat tctcacccag cgccacctct ttcacatgag gggagtttgg
     2881 cagcatggtt atgatgacgt cgcactgttc agcgatcgct ttagccgtag acgctgtttc
     2941 tgcacctgca gcaatcacgt cagcaatagc ttctgggtta cggtcagcaa ccaccagcga
     3001 gtaacctgct ttcagaaggt ttttactcat tggtttaccc ataatcccca ggccaataaa
     3061 accaactttc atagtcatat caatcatctc tcttgttgcg gtggtggtta ttttttaaag
     3121 gtatcagcca gtttctgagt ggcagagcgg aagacgccga gatcgctgcc gacagccaca
     3181 aacgtcgcgc cccattccag ataacgacgc gcatcggctt cgatcggtgc gaggataccg
     3241 ctgggtttgc cgtgcgcact ggcacggtta aaaatgtgct gaattgcttt ttgtacatcc
     3301 gggtgcgagg cattgccgag atggcctaat gccgcagcca gatcgctggg gccgacgaag
     3361 atgccgtcta cgccttcggt agcggcaatg gcatcgatgt tatctacgcc ctgctgactt
     3421 tctatctgga ccagaatagt gatgttcttg ttcgactgag cgaaataatc cgccacggtg
     3481 ccaaacatat tggcgcggtg agaaacggag acgccgcgaa tgccttccgg tgggtaacgg
     3541 gttgatgcca ccgcctgctc tgcttcctct tttgtttcta caaaaggaat caggaagtta
     3601 tagaaaccga tatccagaag acgcttaata attaccggct cgttggtcgg cactcgcact
     3661 actggcgcgc tggcgctgcc tttcagggcc attaactgcg gaataaacgt ggaaatatcg
     3721 tttggcgcat gttcgccatc cagcaccagc cagtcaaacc cagccaaacc aagaacttca
     3781 gtgctaatcg ggttagagag tgctgaccag caaccaattt gtacctgttt cgcagccagt
     3841 gcggctttga atttattcgg gaaaacatcg ttattcatcg cttatacctt tgcttatttc
     3901 tgcaattcca tacgtttaat gtcgccaact acgaagaggt agcagaccat cgccatcagc
     3961 gctgaacatc ccacgaaaac cagtgctgca ttgaaggagt gcagttcact taccaggtag
     4021 ccaatcacca gtggagtgac aatggaggca acattgccaa agacgttaaa gacgccgccg
     4081 cagaggccaa caatctcttt cggcgcggtg tcagaaatca ccgaccagcc cagagcacca
     4141 aatcctttgc caaaaaaagc cagcgccatc agcatgacca ccagcgtggt gttgttggtg
     4201 tagttacata agatgatggt ggaagccagc aacattccca gcacaatcgg tagcttacgt
     4261 gccagggtca gggataaacc gcgtttgatc agataatccg agaagacacc tcccagcacg
     4321 ccgcccgcaa aaccacacag tgccggaatc gaggcgacca gacccacttt cagaatcgac
     4381 atgccttttt cctgcaccag ataaatcggg aaccaggtga ggaagaacca ggtgatggtg
     4441 ttgataaaat attgtccgaa aaatacgccc aacatcatgc ggttagagag caattgcttg
     4501 atgtaatgca gtttgggacc gcttgctgcc gcgctgcccg gctttttgtg gtccatatcg
     4561 accaccgcgc cattttcaga gataaacttc agctcttccg cagacatacg tgggtgatct
     4621 gtcgggttat gaatcaactt gatccacagc gccgtcagca caaaaccaat cacccccata
     4681 acggtgaaga cgtgctccca gccccaggcg aaagtcagcc agccaagcag cggcgaaaag
     4741 agcgccagcg agaaatattg cgccgagtta aagatggcgg aggcagtacc acgttctttc
     4801 gtcgggaacc aggcggcgac aatacgggcg ttcgccggga atgatggcgc ttccgagaac
     4861 cccagcataa agcgcataaa gaacatggag atccctgccc aggccagcgg gaacatatca
     4921 acaaagcctt gcaggaaggt gaacagcgac cagaaaaaga ggctgtaggt gtaaactttt
     4981 ttcgagccaa acttatcaag cagccagccg ccgggaattt gcatcagcag gtaggcccag
     5041 ccaaaagcgg agaagatgta acccatcgaa accgcactta actgcaactc ttttgccact
     5101 tcggtaccag caatagacag cgttgcacga tcggcgtagt taacggcggt aacaataaaa
     5161 ataatcagta atattaaata gcgggtatgc acgcctttct tttttacgtc aacggtgtcc
     5221 agaatcattt tatttacctc gggtacttat gctgattttt attattatgg tgaaggtgtt
     5281 atttatgagt tttatttatg ccgttacgac aatgaactcg ggaattagta taagcagcgc
     5341 gagaataata atcattgtgc aaatgctaat ttaattaata ccatttaaat attattttga
     5401 gcatatgcac ataaggttgc gcgctaaagc acagatttgc gctttacctt gccgggcggc
     5461 actgcaatcc ctgaaatgat tgacattgat cacatttctg cgtttaaact cctgacattc
     5521 ttatttcacc caatgaagtc atttattttt aaatgagacc aggtcctcat tttaataacc
     5581 cctggctgga gaatattgca caatggccaa catcgaaatc agacaagaaa cgccaactgc
     5641 gttttatata aaagttcacg acacagataa tgtggcaatt attgttaatg ataatggcct
     5701 gaaagcagga acgcgttttc cggatgggct ggaattaatt gaacatattc cccaggggca
     5761 taaagtcgca ttgctggaca ttccggctaa tggtgaaatt attcgttatg gcgaagtgat
     5821 tggttacgcc gtgcgcgcaa tcccacgcgg aagctggatc gacgaatcaa tggttgtact
     5881 accggaagcg ccgccgttac acacgctgcc actggcaacc aaagtcccgg aacccttacc
     5941 gccgctggaa ggatacacct ttgagggcta tcgcaatgcc gatggcagcg tgggcaccaa
     6001 aaacctgctc ggcatcacca ccagcgtcca ctgtgtggca ggcgtggtgg actacgtagt
     6061 aaaaatcatt gaacgcgatc tgctaccgaa atacccgaac gtcgatggcg tggtggggct
     6121 gaatcatttg tacggttgtg gcgtggcgat taacgcaccg gcggcagttg tgcctattcg
     6181 taccattcac aatatttcgc tgaaccctaa ctttggcggc gaagtaatgg tgattggcct
     6241 gggttgtgaa aagttgcagc ctgagcgcct gctgactgga acggatgatg tgcaagctat
     6301 tccagtagaa agcgccagca ttgtcagttt gcaggatgaa aagcatgtcg gttttcagtc
     6361 catggtcgag gatattttgc aggtcgccga acgccatcta caaaaactga atcaacgtca
     6421 gcgagaaacc tgtccggctt cagaactggt tgtcggcatg cagtgcggtg gcagcgatgc
     6481 cttttctggc gtaacggcaa acccggcggt tgggtatgcg tctgatctac tggtgcgctg
     6541 cggcgcaacg gtgatgttct cagaagtcac ggaagtgcgt gacgcgatcc atctgctgac
     6601 accacgcgca gtgaacgaag aggtcggcaa acggctgctg gaggagatgg agtggtacga
     6661 taactatctc aatataggaa aaaccgaccg cagcgccaac ccttcgccgg gcaacaagaa
     6721 aggcggtctg gcaaacgtgg tagagaaggc actcggctcc attgctaaat cgggtaaaag
     6781 cgcaattgtt gaagtgctgt cgcccggtca acgcccgact aaacgcggat taatttacgc
     6841 cgcgacgcca gccagcgatt ttgtctgtgg cacgcaacag gtggcttcgg gtatcaccgt
     6901 gcaagtgttt acgaccggtc gtggtacgcc gtacggcctg atggcggtac ccgtcattaa
     6961 aatggcgacc cgcaccgagc tggcgaaccg ctggtttgat ttaatggata ttaacgcggg
     7021 cactatcgcc accggcgaag aaaccattga agaggtgggc tggaagttgt tccactttat
     7081 tctcgacgtc gccagcggga agaagaaaac cttctcggat caatgggggc tgcataacca
     7141 gctggcagtg tttaacccgg caccggtgac ctgatttctt ttggaacgtc cctcgcaaaa
     7201 catggcctta gtgccatgtt tttattgttt aaagccccca cgtccattaa taatgcattt
     7261 gcattacctt agttcaagct tataatttga gcagaaaaca ggctgtaaaa ggacagtgaa
     7321 tcatgcccgc taatgctcgc tctcacgctg tactgaccac tgaatcaaag gtcacgatac
     7381 gcggacaaac aactatcccc gcgccagtgc gtgaggcctt aaaactgaag ccaggccagg
     7441 acagcattca ttacgaaatt ctgcctggtg ggcaagtctt tatgtgccga ctgggagatg
     7501 aacaggagga tcatactatg aatgcatttt tgcgttttct ggatgcagat atccagaaca
     7561 acccgcaaaa aactcgtcca ttcaacattc aacaaggaaa gaaacttgtc gctggcatgg
     7621 acgtcaacat tgatgatgag attggcgacg acgaataatg gattttccac aaagggttaa
     7681 tggttgggcg ctatatgctc atccctgttt tcaggaaacc tacgatgctt tagttgccga
     7741 agtcgaggca ttaaagggaa aagatcctga aaattatcag agaaaagctg ccacaaagtt
     7801 attggcggtg gtccataaag tgattgagga gcatatcacg gtcaatccat catcaccggc
     7861 attccgtcat ggcaagtcgt taggctctgg gaaaaataaa gactggtcac gggtaaaatt
     7921 tggtgctggt cgctatcgtc tcttctttcg ttacagtgaa aaagagaaag tcatcattct
     7981 gggatggatg aacgatgaaa acacgctgcg cacctacggt aaaaaaacag atgcctatac
     8041 cgtattcagt aaaatgttaa aaagaggaca tcctcctgcc gactgggaaa gcctcaccca
     8101 agagacagaa gaaaaccatt gatggtgttc acaatgcccg ccgaacgtat tcgtgttgac
     8161 cagcgggcac cctttctcac tccccgacca gaatcacttc aaccccagcc tttcgcagtc
     8221 cttccagact atccgtagga atgccttcat caacaatgat catgtcgata cgttgagtat
     8281 caatgatctt atgtaaactg gaacgattga acttactgga atcggtgact acgatgatcc
     8341 gttccgcaac ttcgcacatc cggcggttta aacgggcttc atcttcatta tgcgtgctga
     8401 cgccgcgctc cagatcgatc gcatctacac caagaaacag catatcgaag tggtaatttt
     8461 gcagcgattg ttcagcctga tcgccgtaaa aagattgcga ctgacggcgc aaatgcccgc
     8521 cggtcatcag cagctcaacg ccttccgctt ccagcaaagc attagccacg ttcataccgt
     8581 tggtcatcgc aattacgtca gtgtgcttgc gcatcagacg agcaatctca aaagtggtag
     8641 tcccggaatc gaggatcacc cgatgacctg gctgaatcaa ctcaacggca gctttcgcaa
     8701 cgctgcgttt catcgcggtg ttcagtgcgc ttttatcttc cactgatggc tcgactgacg
     8761 gcgtcgtgct atcgcagatc aacgcgccac cataggcacg cacagcgatc ccctgctttt
     8821 ccagaaacgc cagatcgttg cggatcgtca cggtagatac gccatacaat gccgacagat
     8881 cgttaacctg cacactccct tgctgtcgca gacgctgaat gatctgttct cgtcgctcgc
     8941 tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt actcatagta agtcctttcg
     9001 taaaactttc gtttcatttc gttttgccta ttaacgcctt tctattaagc aaatgcaagc
     9061 ccaccttgcc cattgacgca agctactctc gtttcagtga ctttcattat gtttcttttg
     9121 tgaatcagat cagaaaacta ttatctttcg ttttattttt atctcaccat gacgcagtat
     9181 caactgaaac aaaacgaaag attaatatcg cagtaatctg aactggagag gaaagtgaaa
     9241 catctaacag aaatggtgag acagcacaaa gcgggcaaaa caaatgcaat ttatgccgtt
     9301 tgttccgcac atccgctggt gctggaagct gcaatccgct acgccagtgc aaaccaaacg
     9361 ccgttactga ttgaagcaac ctccaatcag gtagaccagt tcggcggtta taccggaatg
     9421 acgcccgccg attttcgcgg ctttgtttgt cagctcgccg actcgttgaa tttcccgcag
     9481 gatgcgttga ttctgggtgg tgaccatctg gggccaaacc gctggcaaaa cctgccagcc
     9541 gctcaggcaa tggccaatgc cgatgatttg attaaaagct acgttgcggc aggattcaaa
     9601 aaaatccacc ttgattgcag catgtcctgt caggacgatc caattccctt aactgatgac
     9661 atcgtggctg aacgcgccgc ccgtctggcg aaagtggcgg aagaaacctg tcttgaacac
     9721 tttggcgaag ccgatctgga gtatgtcatt ggtaccgaag tgccggtacc tggcggcgcg
     9781 catgaaacct taagcgagct ggcggtcacc acgccggatg ccgcccgcgc cacgctggaa
     9841 gcccatcgtc acgcctttga aaagcaaggt ttgaatgcca tctggccacg catcattgcc
     9901 ctggtggttc aacccggcgt cgaattcgat cacaccaacg ttattgatta tcagcccgcc
     9961 aaagcgagcg ccttaagcca gatggtcgaa aactacgaaa cgctgatttt cgaagcgcac
    10021 tctaccgatt atcaaacgcc gcaatcgctg cgccagctgg tgattgacca ctttgccatt
    10081 ctgaaagttg gcccagcgct gaccttcgcc ctgcgtgaag ctctgttctc tctggcggcg
    10141 attgaagaag aactggtgcc agcgaaagcc tgttctggtc tgcgtcaggt gctggaagac
    10201 gtgatgctcg accgcccgga atactggcaa agccactacc acggtgacgg caacgcgcgt
    10261 cgtctggcgc gtggttatag ctactcggat cgcgtgcgct attactggcc ggacagccag
    10321 attgatgacg ctttcgctca tctggtacgt aatctggcgg attcaccaat tccgctgccg
    10381 ctgatcagcc agtatctgcc gctgcagtac gtgaaagttc gctccggcga gctgcagcca
    10441 acgccacggg aactcattat caaccatatt caggacatcc tggcgcagta ccacacagcc
    10501 tgtgaaggcc aataagcaaa acaaagagga acacgctatg ccaaatattg ttttaagccg
    10561 gattgatgaa cgcttgattc acggtcaggt cggcgttcaa tgggtcggat ttgcgggggc
    10621 aaatctggtg ctggtagcca acgatgaggt tgccgaagat ccggtacaac aaaacctgat
    10681 ggaaatggta ctggcagaag ggatcgccgt acgtttctgg acgctgcaaa aagttatcga
    10741 caacattcat cgcgccgccg atcgacagaa aatcctgctg gtttgtaaaa cacccgccga
    10801 tttcctgacg ctggtgaaag gtggcgttcc ggtgaatcgc attaacgttg gcaatatgca
    10861 ctacgccaat ggcaaacaac aaatcgccaa aacggtttct gtggatgcgg gcgatatcgc
    10921 agcatttaac gacctgaaag ccgctggggt ggaatgcttc gttcagggcg tcccgacaga
    10981 gcctgctgtg gacctcttta aattactttg agggattcat catggaaatc agcctgttgc
    11041 aggcatttgc gttgggcatt atcgccttta tcgctggcct ggatatgttt aacggcctaa
    11101 cacatatgca ccgcccggtg gtcctcggcc cgttggtcgg gctggtactt ggcgatctgc
    11161 ataccggaat tttaaccggc ggtacgctgg aactggtgtg gatggggctg gccccgctgg
    11221 cgggcgcaca gccgcctaac gtgattatcg gtactatcgt cggcacggcg tttgccatta
    11281 ctactggcgt gaaacccgat gtcgcagtcg gtgtcgccgt acctttcgct gtcgcagtac
    11341 agatggggat taccttcctg ttctcggtga tgtccggcgt gatgtctcgc tgcgaccgga
    11401 tggcggagaa cgccgatact cgcggcattg aacgcgtgaa ctatctggca ctgctggcac
    11461 tcggcacttt ctattttctc tgcgctttcc tgcctatcta cttcggcgcg gaacatgcaa
    11521 aaaccatcat tgatgtcctg ccgcaacgat taatcgacgg cctcggcgtc gcgggcggca
    11581 tcatgccagc aatcggtttt gccgtgctgc tgaaaatcat gatgaaaaac gtctacatcc
    11641 cctacttcat cctgggcttt gttgccgcag cctggctcaa gttaccggtg ctggctattg
    11701 ctgccgctgc gctggcgatg gcgctgatcg acctgctgcg taaatctcct gaaccgactc
    11761 aacctgcggc acagaaagag gaatttgaag atggcatcta atcaaaccac cctgccgaac
    11821 gtcagtgaaa acgaagaaac attgctgact ggcgtcaatg aaaacgtgta tgaagatcag
    11881 agcattggcg cggagctgac gaaaaaagat atcaaccgcg tcgcctggcg ttccatgctg
    11941 ttacaagcgt cttttaacta cgaacgtatg caggcttctg gctggttgta cggtctgctg
    12001 cctgctctga aaaagatcca cactaataaa cgcgacctgg cgcgcgccat gaaggggcat
    12061 atgggtttct tcaataccca tccgtttctg gtgacatttg ttatcggcat tatccttgcg
    12121 atggagcgtt ctaagcagga cgttaacagt attcagagca ccaaaattgc cgtcggtgcg
    12181 ccgctcggcg ggattggcga tgcaatgttc tggctaacgc tactgccgat ttgtggcggg
    12241 attggagcca gtctggcatt gcaaggctcc attcttggcg cagtcgtctt tattgtgctg
    12301 ttcaacgtgg tacacctggg gctgcgtttt ggtctggcgc attatgctta ccgcatgggc
    12361 gtggcggcga ttccactcat taaagcgaat accaaaaaag tcggccatgc tgcgtcaatc
    12421 gttgggatga cggtaatcgg cgcgctggtg gcaacctatg ttcgtttgag caccacgttg
    12481 gaaatcacag cgggcgacgc agtggttaag ttacaggctg atgttatcga caaactgatg
    12541 cctgccttct taccgttggt ctacaccctg accatgtttt ggttggtacg ccgcggctgg
    12601 agtccgctgc gtctgattgc agttaccgtg gttctcggca tcgtcggtaa attctgccat
    12661 ttcctttaaa tacaatgagg tattagatgt taagtattat tttgacaggg catggcggat
    12721 ttgccagcgg catggaaaaa gcaatgaagc aaatcctcgg cgagcaatcg cagtttatcg
    12781 ccatcgattt tccggaaacc tcaagcaccg cgctgcttac atcgcagctt gaagaggcca
    12841 tcgcccaact ggattgtgaa gacggcattg tttttctgac ggatttgctg ggcggcacgc
    12901 cgtttcgcgt agcttcaacg ctggcgatgc aaaaaccggg ctgtgaagtg atcaccggca
    12961 ccaatttgca actgctactg gagatggtgc tggagcgcga agggttaagc ggtgaagagt
    13021 tccgtgtgca ggtgctggag cgcgaagggt taagcggtga agagttccgt gtgcaggcgc
    13081 tggagtgtgg acaccgtggg ctgaccagtc tggtagacga gttaggccgc tgccatgaag
    13141 aatgtccggt cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga
    13201 agagggatgg ctcgatgacc atcagttgtg tattgctgac ggtgtaatcg cagcaatcga
    13261 accgattcca gtgggcgtga ctgaacgcga tgcggaactg ctctgccccg cttacatcga
    13321 cacccatgta cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga
    13381 caagctggca atgcacaagg cacgcgaagg tgtcggcagt tggttgccga ctaccgtaac
    13441 cgcgccgctt aacaccattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg
    13501 cggacctggt gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa
    13561 taaaggcgcg catccgccgg agctgttccg cgagctggaa attgccgagc tggatcagtt
    13621 gattgccgtt tctcagcaca ccttacgtgt ggtagcgctg gcaccggaaa aagaaggggc
    13681 attgcaggcc atccgccatc ttaaacagca aaacgtaagg gtgatgctgg ggcatagcgc
    13741 ggcgacctgg caacaaaccc gcgccgcatt tgatgctggt gccgacggcc tggtgcattg
    13801 ctataacggg atgacaggtt tacatcaccg cgaaccggga atggttggcg cgggattaac
    13861 ggacaagcgc gcctggctgg aactgatagc cgatggtcat catgtgcatc cggcggcgat
    13921 gtcgctgtgt tgttgctgtg cgaaagagag aatcgtgctg atcaccgacg cgatgcaggc
    13981 agccgggatg ccggatggtc gctatacgtt atgtggtgaa aaagtgcaga tgcacggtgg
    14041 cgttgtccgt accgcgtctg gtgggctggc gggcagtacg ctgtctgttg atgcggcagt
    14101 gcgcaacatg gtcgagttga cgggcgtaac gcctgcggaa gccattcata tggcatcgct
    14161 gcatccggcg cgaatgctgg gtgttgatgg tgttctggga tcgcttaaac cgggcaaacg
    14221 cgccagcatc gttgcgctgg atagcgggct gcatgtgcaa caaatctgga ttcagagtca
    14281 attagcttcg ttttgatagt ttgctccttt attgggcctt cacttccccc gtaaggcctt
    14341 tctttttctt tcgttttgat ctgtgcagcg gtgtcggatg cgacgctaac gcgtcttatg
    14401 cgacctacgg tacgccgcat ccagcaatcg gtgaacatag cgacgtcatt acgcaattct
    14461 ttaatcttcc cttgtctttt ctttcaatga tcacagattt cgttttattt cctttttctc
    14521 cattgaactt tcggtttctt ttctatagat tttaatcaac gaaagacatc accaagtgaa
    14581 atgaaacgaa aggcaagtga aagcgacaac gcccgacgtc aagttcatca gactaaggat
    14641 tgagttatgc caaaaaatta cacccctgct gccgccgcaa ccggtacatg gactgaagaa
    14701 gagatccgcc atcagcctcg cgcatggatc cgttcactca ccaacatcga cgcgctacgt
    14761 tccgcgctca ataacttcct tgaaccgtta ctgcgcaaag agaatctgcg gatcatcctg
    14821 accggagccg gaacctcggc atttatcggt gacatcatcg cgccgtggct cgccagccat
    14881 accggtaaaa acttcagcgc cgtaccgacc accgatctgg tcactaatcc gatggactac
    14941 ctgaacccag cccatccgct gctgttgatc tccttcggtc gatccggcaa cagcccggaa
    15001 agcgtcgcag ccgtggaact ggcaaatcaa tttgtaccgg aatgctatca cctgccgatc
    15061 acctgcaacg aagcgggcgc tctttaccaa aacgcgatca acagcgataa cgcgtttgcc
    15121 ctgctgatgc ccgcagaaac gcacgatcgc ggctttgcga tgaccagcag cattaccacc
    15181 atgatggcca gctgcctcgc ggttttcgca cctgagacga tcaacagcca aaccttccgc
    15241 gacgtggcgg atcgttgcca ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg
    15301 tttggttacg caccgtggaa acggatcgtt tatctcggta gcggtggctt acagggcgca
    15361 gcacgcgagt cggcgctgaa agtgctggaa ctgacggcgg gtaaactggc ggccttttat
    15421 gattctccaa ccggattccg tcatggacca aaatcgctgg tcgatgacga aacgctggtg
    15481 gtggtatttg tctccagcca cccttacacc cgtcagtatg atcttgatct gctggctgaa
    15541 cttcgccgtg acaaccaggc aatgcgtgta atcgccatcg ccgcggaaag cagcgacatc
    15601 gtcgctgccg gtccacatat catcctgcca ccgtcacgtc actttatcga cgttgagcag
    15661 gcattttgct tcctgatgta cgcccagacg tttgcactga tgcagtcgct gcacatgggc
    15721 aatacgccgg ataccccatc agccagtggc accgttaacc gcgtggtgca aggcgtaatc
    15781 attcatccgt ggcaggcata agaggatcgc attatgagca ttatctccac taaatatctg
    15841 ttacaggacg cccaggccaa tggctacgcg gtgcctgctt ttaacattca taacgccgag
    15901 acgatccaag cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat cctcgccgga
    15961 acgccgggga cctttaaaca catcgcgctg gaagagatct acgccctgtg tagcgcctat
    16021 tccacaacct acaacatgcc actggcgctg catctcgacc accacgaatc gctggatgat
    16081 attcgccgta aagtccacgc aggcgtgcgc agtgcgatga tcgacggcag ccacttcccg
    16141 tttgccgaga acgtaaagct ggtgaaatcg gttatcgact tctgccactc ccaggattgc
    16201 agcgtggaag cagaactggg ccgcctgggc ggtgttgaag atgacatgag cgttgacgcc
    16261 gaaagtgcat tcctgaccga tccacaagaa gctaaacgct ttgtcgaact gactggcgtc
    16321 gacagcctgg cggtagcgat tggtacggcg cacggcttat acagcaaaac gccgaagatt
    16381 gatttccagc ggctggcgga aattcgtgaa gtggtggatg ttcctctggt gctgcatggt
    16441 gccagcgatg ttccggatga atttgtccgt cgcactattg aacttggcgt cacaaaagtg
    16501 aacgttgcca cagaattaaa aatagccttc gctggcgcgg ttaaagcctg gtttgcggaa
    16561 aatccgcagg gtaatgatcc tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa
    16621 gttgtcagaa ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc ataatcatta
    16681 gttttttatg atttatccca atgtacttcc cgattaatat cggggagtgc cttaatggaa
    16741 aaggagataa ctaaacctta ataaatacat cagtacaata tcgcaacaat aatatattta
    16801 aaaaaattat attattcaac tttatggtga ggattacaca atgagcagtc caaatattct
    16861 cttaacccgt attgataacc gtctggttca tggccaggtt ggcgtgacct ggacatccac
    16921 catcggtgca aatctgctgg tagtcgtgga tgatgttgtc gctaacgatg atattcaaca
    16981 gaaattaatg ggtattaccg cggaaaccta cggctttggc attcgtttct ttactatcga
    17041 aaaaaccatt aacgtcatcg gcaaagctgc accacatcag aagatcttcc tgatttgccg
    17101 tacgccacaa acggtacgta aattggtaga aggtggcatt gacctgaaag atgtcaacgt
    17161 cggcaatatg catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga
    17221 ccaggatctc acggacttac gttttattaa acaacgtggc gtgaatgttt tcattcagga
    17281 tgtccctggc gatcaaaaag aacaaatccc tgactaaatc taaaatcgcc ttaatattgg
    17341 tttgaggtaa taaaaatgca tgaaataacc ctacttcagg gattatccct ggcggcgtta
    17401 gttttcgttc tggggattga tttttggctg gaagccttat ttttattccg cccgataatc
    17461 gtttgtaccc ttacaggtgc tattctcggt gatattcaga ctggcttaat taccggtggt
    17521 ctgacagagt tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaacccg
    17581 attatggcgg gtctgatgac caccgtcatt gcatggtcta cgggcgttga tgccaaaaca
    17641 gcaattggtc ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat
    17701 tccgctttct cattatttat gaccaaagcc gataaatgcg cgaaagaggc ggatacggca
    17761 gcgttttccc ggcttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt
    17821 gctttcctct gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc
    17881 gcctggctga cccacggctt tgaagtggct ggcggtattc tgcctgccgt tggttttggc
    17941 ttgctgctgc gcgtgatgtt caaagcacaa tatatccctt acctgatcgc cggcttcctg
    18001 tttgtttgct acatccaggt cagtaacctg ttgccggttg ccgtactggg cgcaggcttt
    18061 gcggtgtatg agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc
    18121 agtaaaaatg aagaagagga ctacagcaat gggatctgaa atcagtaaaa aagatatcac
    18181 ccgtctgggc tttcgttcgt cactgctgca agcgagcttt aactacgaaa ggatgcaggc
    18241 gggcggtttt acctgggcga tgttgccgat cctgaaaaag atttataagg atgacaaacc
    18301 gggcctaagc gcagcgatga aagataacct cgaatttatt aatacccacc cgaatctggt
    18361 cggattcctg atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat
    18421 taaaggcctc aaagtggcac tgtttggccc aatcgccggg attggcgatg cgattttctg
    18481 gtttactttg ttgccgatta tggcgggaat ttgctcatca tttgccagcc agggaaacct
    18541 gctggggccg attctatttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg
    18601 gacccacgtc ggttattcag tcggcgtgaa ggcgatcgat aaagtacgag agaactcgca
    18661 gatgattgcc cgttcggcaa ccatcctcgg gatcacggta atcggcgggc tgatcgcttc
    18721 gtatgtgcat attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct
    18781 gcagcaggat ttcttcgata aagtcttccc gaacatttta ccgatggcct acaccctgct
    18841 gatgtattac ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt
    18901 tgtgctctct attgtttgtt ccgcattcgg cattttgtaa atggaacgag gcactgcgtc
    18961 tggtggtgcc tctttactta aggaatttca tcctgtgcaa acccttcagc aagttgaaaa
    19021 ctatacggcg ttaagtgaac gtgccagcga atatttattg gctgtgatcc gtagcaaacc
    19081 ggatgccgtg atttgcctgg cgactggagc cacgccatta ctgacgtatc attatctggt
    19141 agaaaaaatc catcagcagc aggttgatgt cagccaactc accttcgtga agctcgacga
    19201 atgggtggat ctgccattaa cgatgcctgg cacctgcgaa actttcctgc aacagcatat
    19261 cgtgcagccg ctggggctac gtgaagacca gctcatcagc tttcgctccg aagagataaa
    19321 tgagacagag tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg
    19381 cgttctcgga ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaagcctgca
    19441 accggcctgc catatcagtc aacttgatgc cagaacacag caacatgaga tgttaaaaac
    19501 cgcgggtcgc cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatgcccg
    19561 cgaagttttg ttactggtga ctggcgaagg aaagcaggat gcgacagaac gttttctcac
    19621 ggctaaagtc tccaccgcta tcccggcttc gtttttatgg ctgcacagta actttatttg
    19681 tttaattaac acctaaccag atgaaaaatt tgctcaaaat gtttaaatta
//
LOCUS       DAPHJE010000085        19640 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPHJE010000085 DAPHJE010000000
VERSION     DAPHJE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.085"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            149..1282
                     /gene="ompN"
                     /locus_tag="RH222_003087"
     CDS             149..1282
                     /gene="ompN"
                     /locus_tag="RH222_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV3798073.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1423..1857
                     /gene="uspF"
                     /locus_tag="RH222_003088"
     CDS             1423..1857
                     /gene="uspF"
                     /locus_tag="RH222_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV3798074.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2033..2968
                     /gene="ttcA"
                     /locus_tag="RH222_003089"
     CDS             2033..2968
                     /gene="ttcA"
                     /locus_tag="RH222_003089"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV3798075.1"
                     /translation="MSQNQEISKKGQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGINHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3097..4470)
                     /gene="dbpA"
                     /locus_tag="RH222_003090"
     CDS             complement(3097..4470)
                     /gene="dbpA"
                     /locus_tag="RH222_003090"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV3798076.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4948..5931)
                     /gene="zntB"
                     /locus_tag="RH222_003091"
     CDS             complement(4948..5931)
                     /gene="zntB"
                     /locus_tag="RH222_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV3798077.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6186..7418
                     /gene="dgcM"
                     /locus_tag="RH222_003092"
     CDS             6186..7418
                     /gene="dgcM"
                     /locus_tag="RH222_003092"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV3798078.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITERKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7439..8002)
                     /gene="smrA"
                     /locus_tag="RH222_003093"
     CDS             complement(7439..8002)
                     /gene="smrA"
                     /locus_tag="RH222_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV3798079.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8332..9240)
                     /locus_tag="RH222_003094"
     CDS             complement(8332..9240)
                     /locus_tag="RH222_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3798080.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9416..10726
                     /gene="abgA"
                     /locus_tag="RH222_003095"
     CDS             9416..10726
                     /gene="abgA"
                     /locus_tag="RH222_003095"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV3798081.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10726..12171
                     /gene="abgB"
                     /locus_tag="RH222_003096"
     CDS             10726..12171
                     /gene="abgB"
                     /locus_tag="RH222_003096"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV3798082.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12208..13734
                     /gene="abgT"
                     /locus_tag="RH222_003097"
     CDS             12208..13734
                     /gene="abgT"
                     /locus_tag="RH222_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDV3798083.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13745..14260
                     /gene="ogt"
                     /locus_tag="RH222_003098"
     CDS             13745..14260
                     /gene="ogt"
                     /locus_tag="RH222_003098"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV3798084.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14455..15207
                     /gene="fnr"
                     /locus_tag="RH222_003099"
     CDS             14455..15207
                     /gene="fnr"
                     /locus_tag="RH222_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV3798085.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15359..16309
                     /gene="uspE"
                     /locus_tag="RH222_003100"
     CDS             15359..16309
                     /gene="uspE"
                     /locus_tag="RH222_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV3798086.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16359..16616)
                     /locus_tag="RH222_003101"
     CDS             complement(16359..16616)
                     /locus_tag="RH222_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV3798087.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16860..17891
                     /gene="ynaI"
                     /locus_tag="RH222_003102"
     CDS             16860..17891
                     /gene="ynaI"
                     /locus_tag="RH222_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV3798088.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(17942..19555)
                     /gene="mppA"
                     /locus_tag="RH222_003103"
     CDS             complement(17942..19555)
                     /gene="mppA"
                     /locus_tag="RH222_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV3798089.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAVEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT         5002 a         4852 c         4721 g         5065 t
ORIGIN      
        1 attatcattt cagaggaatt atttgattaa ggttttactt aaggcgtaac aaatgatatt
       61 tatcggctaa ctgaacttct cctttaggat gttttcactc cccgaaatgg ggataaaagg
      121 caaataaaat aactaaagga tttattcaat gaaaagcaaa gtactggcac ttttaattcc
      181 tgccctgctc gccgcaggtg ctgcacatgc agccgaagtt tataataaag acggcaacaa
      241 attagatctg tatggcaaag ttgatggcct gcattatttt tctgataatt cggcgaaaga
      301 tggcgaccag agctatgcgc gtctgggttt taaaggcgaa acccaaatta acgatcaact
      361 cactggctac ggtcaatggg aatacaatat tcaggcaaac aacactgaat cttcaaaaaa
      421 ccagtcatgg acccgtctgg catttgccgg gctgaaattt gcagattacg gttctttcga
      481 ttacggacgt aattatggcg taatgtacga catcgaaggc tggaccgata tgctgcctga
      541 atttggcggt gactcttata ccaatgcaga caactttatg actggtcgag ccaatggcgt
      601 cgcgacttat cgtaatactg atttcttcgg tctggtaaat ggtctgaact tcgcggtgca
      661 gtatcaaggt aacaacgaag gtgccagtaa tggtcaggaa ggcaccaaca acggacgtga
      721 tgttcgccat gaaaacggtg acggctgggg tctttccaca acatatgatt taggcatggg
      781 ctttagcgct ggtgcggcat acacctcttc tgaccgcacc aatgatcagg ttaaccatac
      841 tgcggcgggt ggtgataaag cagacgcgtg gactgctggg ctaaaatacg atgctaacaa
      901 tatttacctg gcaaccatgt attcagaaac gcgtaatatg accccgtttg gcgacagcga
      961 ttatgctgtt gcaaacaaaa cccagaactt tgaagtcact gcacagtacc agtttgattt
     1021 tggtctgcgt ccggcagtct ctttcctgat gtctaaaggc cgtgacctgc acgctgccgg
     1081 tggtgcagac aacccggcag gtgttgatga taaagatctg gttaaatacg ccgatgttgg
     1141 cgcgacttac tatttcaata aaaacatgtc cacttatgtt gactataaaa tcaacctgtt
     1201 ggatgaagat gacagtttct acgctgccaa tggcatctct accgatgata ttgtcgcttt
     1261 aggtctggtt tatcagttct aaatcctcct gcccgctgtt atggcgggct ttttctgctt
     1321 attcttcctc ctttgatcta aattaaaaat gtgaactccg tcattacaca aaaagtgtca
     1381 tctggcgtta cactttatgc ggatactaaa acaggaggtt ttatgaacag aacgattctt
     1441 gtccctatcg atatttccga ttcagaatta actcaacgcg tgattagcca cgttgaggca
     1501 gaggcaaaga ttgatgatgc agaggttcat ttcctgacgg taataccttc actgccctac
     1561 tatgcctctc tgggtttagc gtattccgca gaattaccgg caatggatga cctgaaagcg
     1621 gaagccaaat cgcaactgga agagatcatt aaaaaattta aactgccaac cgacagagtg
     1681 catgtccatg ttgaggaagg ctcgcccaaa gaccgcattc tggaattggc gaagaagatc
     1741 cccgctcata tgatcatcat tgcttcccat cgaccggata tcaccactta tctgctcggt
     1801 tccaacgccg cagctgtagt gcgtcacgca gagtgctccg tgctggttgt gcgctgacac
     1861 taacgcccgc acattgctgc gggctttttg attcatttcg caaatgtgct gacattttcc
     1921 cctctaatcc gtaccataca cgccacagtt tttatatcag atttcttatg ctgggcgttc
     1981 cggcatgacg catactcttc tgatgccata taacgaattg agtcgctttt aaatgtcgca
     2041 aaatcaagaa attagtaaga aaggacaata caatctgaac aaattacaaa aacgtctgcg
     2101 tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt gaagaaggcg atcgcatcat
     2161 ggtttgcctc tcagggggta aagacagcta taccatgctg gagattctgc gcaatttgca
     2221 gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg
     2281 cttcccagaa cacgttctgc ccgagtatct tgaaaagctg ggcgttgagt acaagattgt
     2341 cgaagagaac acttacggta tcgtgaaaga gaagattcca gagggcaaaa ccacttgctc
     2401 actgtgttct cgccttcgtc gcggtatcct ttatcgtacc gcaacggaac tgggggcgac
     2461 gaagatcgcg ttgggtcacc atcgtgacga tatcctgcaa acgttgttct taaatatgtt
     2521 ctacggcggc aagatgaaag gtatgcctcc gaaactgatg agcgatgatg gcaaacatat
     2581 cgttattcgt ccgctggcct actgccgcga gaaagatatt cagcgatttg ccgatgcaaa
     2641 agcgttcccg attattccgt gcaacctgtg cggttcacag cctaacctgc aacgtcaggt
     2701 gattgctgac atgttgcgtg actgggataa acgttatcca gggcgtatcg agacgatgtt
     2761 cagcgcgatg cagaatgtag tgccgtcgca tctgtgcgat accaacctgt tcgattttaa
     2821 aggcattaac cacggttctg aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga
     2881 gatcccacta caaccggcgg gctggcagcc agaagaagat gaaaatcagt tggatgagtt
     2941 acgactgaat gtggttgaag tgaaataacc aggatagcgc ccgatgcgca agcgtattgg
     3001 gctactctta tggaggccgg ataagacgcg gccagcgtcg catccggcaa tcccgaataa
     3061 gatgtttact cttgcacccg gcaattcaac atttcattat tttaataacc gcacccggca
     3121 cgtttttcct ttaatcttcc cgccctgtaa ctgtttccat gctttatgag caacagcctg
     3181 acggaccgcg acatagacat gcgccggatg cacggcgatt ttgccaatat ctgcgccatc
     3241 aagcccgata tctcctgtca gtgcacctaa tacatcaccc gggcgcattt tggctttttt
     3301 cccgccatcg atacacaacg ttgccatttc tgcttccagc ggcacaatgg aactattagc
     3361 tggcggcgtt tgccagttaa gttttatctg caacatgtca gaaatgatat tggcccgctg
     3421 tgcttcttcc ggagcacaga aactgatcgc cagaccgcta tttcctgcac gagctgtacg
     3481 accgatgcga tgtacatgaa cttcagggtc ccacgccagc tcaaagttca ccaccagctc
     3541 aagcgattta atatccagac cacgcgcagc aacatcagta gcaaccagta cacgggcgct
     3601 accgttagca aaacgtacca gagtctgatc gcgatcgcgt tgctccagat cgccgtgtaa
     3661 cgacaatgca ctttgcccta cttcattcag cgcgtcgcag acagcctggc aatctttttt
     3721 ggtattgcaa aacaccacgc aagaggatgg ctgatgcaag cttaataacc gttgcaacag
     3781 aggaattttg cctttgctgg atgtctcata aaattgttgt tcaatgggtg gcaaagcatc
     3841 tgttgagtca atttcaatcg ccaaaggatc gcgttgcact cgtccgctga ttgcggcgat
     3901 ggcttccggc caggttgccg aaaacagaag cgtctgtcga gatgcaggcg caaaacggat
     3961 gacatcatca atggcatcgc taaatcccat atccagcatg cggtcggcct catccatcac
     4021 caaagtattc agcgcatcca gtgataccgt gcctttttgc aagtgatcca gcaaacgacc
     4081 cggcgttgcc acgataatat gcggcgcatg ttgcaacgaa tcacgctgca taccgaacgg
     4141 ttgaccaccg cacaacgtca aaattttggt atttggcaga aaacgcgcca gccgacgcaa
     4201 ttcacctgcc acctgatccg ccagttcacg cgtaggacac agcactaaag cctgggtttg
     4261 aaatagcgac gcatcaattt gctgtaacaa gccgaggcca aaagccgccg ttttgccgct
     4321 gccggttttc gcctgcacgc gaacatcttt tccggcaagg atcgccggaa gcgcggcggc
     4381 ctgcaccggc gtcatggtta aataacccaa ctcattaagg ttcgtgagtt gggcgggagg
     4441 caaaacattc agggtagaaa aagcggtcac aatctattct cgtggtcatc gacgcaaagt
     4501 tagcaggcgc gtatcctcgc agatctacgc tcacgatgcg acaatttaat cggttcttca
     4561 tcaggtggtg ggtcaggcat gggttgcggg cgagggatcg gatcgggcac tggaacagga
     4621 tcgccaggaa tcggttcagg gacaggaatt tgcaaataaa taagtgtcgt catatttccc
     4681 tccggtcatt gggtggactc ttaaagggta gacgctgata aataacaggc aaaaaaaagc
     4741 cgactcatca aagtcggcgt cgtacgaatc aattgtgcta tgcagtaatt caaaaaagga
     4801 agtaagacaa tatggagcgc aacgcccatc gcttgacgtt gcattcacct gcaagggaga
     4861 tattgccctg aatgggtaga gagtttattg acttcgctca aactttgcgg cgtttttgta
     4921 tacagacagc cggaaaaatt gcttttgtta caaccattta ctacgatgca accataaagc
     4981 aacaccacca ataagaacaa ctaacagaat acaaaaaatt gaaaatccga attgccaccc
     5041 gccgccaggg atcccaccaa ggttgacgcc aaataaccct gtcagaaagg tactgggtaa
     5101 aaagaccatt gccatcaatg acattgtata ggtacgacga gctaaatttt cctgcatcac
     5161 ctgagcgatt tcatccgcca tcacgccagt ccgtgctata caggcgtcga tttcgtcaag
     5221 accgcgccca aggcgatcgg caatatcctg catccgacgg cgttggtcat cgctcatcca
     5281 cggcaaacgt tcactggcaa gacgagcata aacatcacgt tgcggtgcca tatagcgacg
     5341 catcacgatt aattgtttgc gcagcagagc caggaatcca cgcggtggaa tttgctgatc
     5401 aaggagatta tcttcaaggt cgataatttt atcgtgcagc tgctcgataa attcgctgga
     5461 atgatcggtc aacgcatcgc acacatccac cagccaccct ccgcaatcgg tcggacccgt
     5521 gccctcttcc agatcgctca ccacatcgtc cagcgccagc actttgcgtt gtcgggtcga
     5581 aacaattaac cgcccgtcca tatatacacg catggcgacc agttgatcgg ggcgttcatc
     5641 ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct tcaccgagac ggctgactcg
     5701 gggccgagtg ctctcgcctg ccagcgcatc acgtacgtta ttgggaagca gcggtgttgt
     5761 cgccagccat tgggcgcttt catggtgtac ataattaagg tggagccagc agggatgcgc
     5821 ttcatcaatc acatctgtat tttccagcgg tttaacgccg cctctaccat ccagcatcca
     5881 ggcaaatact gcatccggga cattaacgtc cgatccctta atcgcttcca cagtgcctcc
     5941 atcatcaacg cattattttg tagtctagcc ttctggccct gttacgcaac atctcatcac
     6001 cccattaccc tgaaatgatt aataaaattc tgtctaaatt gaatacaaaa agcaaaatgc
     6061 ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct atcattatca gaattatctg
     6121 atcatatgac gtggcttttt tgcgatcgga tagcaacaaa aattgataaa aataacggga
     6181 tctcaatgat tacgcacaac ttcaataccc tggacttact caccagtcct gtctggattg
     6241 tttcgccctt tgaggaacag ttaatttatg ccaatagcgc ggcgcgactg ttgatgcaag
     6301 acctcacgtt tagtcagcta cgaaccggac cctattccgt ctcctcacaa aaagaactgc
     6361 cgaaatacct ctccgatctg caaaaccaac acgatattat cgaaatcctc actgttcagc
     6421 gtaaagaaga ggaaacagca ttaagctgtc ggcttgtttt gcgagagctg acagaaacag
     6481 aaccggtgat tattttcgaa ggtatcgaag cgccggcaac gctgggttta aaagccagtc
     6541 gctcggcaaa ttatcagcgc aaaaaacaag gtttttatgc gcgctttttt ctgactaact
     6601 ctgcaccaat gttgttgatt gacccctcac gagatggcca aatcgtcgat gctaacctcg
     6661 ccgcgctcaa tttctatggt tataaccatg aaacgatgtg ccagaaacat acctgggaaa
     6721 ttaatatgct cgggcgtcgc gtcatgccta tcatgcatga aatttcgcat ttacccggcg
     6781 gtcataaacc tttgaatttt gttcataaac tggcggatgg atctactcgt catgtgcaga
     6841 cctatgccgg accgattgaa atttatggcg acaagctcat gttatgtatt gtgcatgata
     6901 ttactgagcg aaaacggctg gaggagcagc tggaacatgc tgctcaccat gacgcgatga
     6961 ccggattact gaatcggcga cagttttatc acattaccga gccaggccaa atgcagcatc
     7021 tcgccatcgc tcaggattac agcttgttgc tcatcgacac cgatcgtttt aaacacatta
     7081 acgatctcta tgggcattct aaaggtgatg aggtgttatg cgccctcgcc cgcacccttg
     7141 aaagttgcgc tcgcaaaggc gatttggtgt ttcgttgggg aggcgaagag tttgtcttat
     7201 tgctaccaag aaccccactg gataccgcgc tttcgctggc tgaaactatc cgtgtaagcg
     7261 tggcaaaagt gagtatttcg ggcttaccac gctttaccgt cagcattggt gtggcgcatc
     7321 acgaaggaaa tgaaagcatc gatgaactgt ttaaacgcgt tgatgatgct ttgtatcggg
     7381 cgaaaaatga tggacgcaac cgcgtgctgg cggcataagc cgcggatgcg tctcgagatc
     7441 aacgactgcg cttagcgtgg cgctcccagt tttcttgctt cgcctgcgcc gttttacgta
     7501 gtgcgacgta acacgccccg ctgccgccat gatgcggtag cgcagtgcaa tatgcctgaa
     7561 catcatcaaa ttcggtcagc cagcgcgcca catagctgcg gacaatattg gcatgcgatt
     7621 tatcatcccg tcctttgcca tgaataatca gcacgttacg caaaccatca gccatggctt
     7681 gttgaatgaa actgaacatc attttgcggc actcttccac cggctggcgc aaaagattca
     7741 ggctcgcctg ttgcggatat ttaccactgc gcagcttatc cagcacccca tgttgcaacc
     7801 cttcccgccg aaactccagc ggctgactta gtgggatgat gtcgagaaat ccggtggtga
     7861 ggaaattatc aagctgcagc gtgtcgatac gctgcggcgc acgttggtta cgcgttggat
     7921 gccagtggac atcggtagca cgtttcagcg gctggacatc ttccatggcg tcaagaaaca
     7981 gcgatttgtc gtcaaggttc atgttacatc ctcccgcaat taagagcgcg atatgataac
     8041 cagaccgggt cggtccaaca acgtattacc caaaattcca gtaataagtt ccaaatattg
     8101 ccgatatttt aagcaaaatg cttatgcatg attattcatt cacgatatta ataatgtaac
     8161 ttatattttc gtgaaatctg tcactgaaga aaattggcaa ctaaaggtta aaaccgttat
     8221 aacacagtca ccggcgcaga ggagacaatg ccggacttaa gacgcggatg cactgctgtg
     8281 tgtactgtag agtctggcgg atgtcgacag actctatttt tttatgcagt tttaactttg
     8341 cagatagccg cattctcgcc ggaattgcgt gattaatgac gcggtcagtg gtgtctggcg
     8401 actatcacgc cgctgaatca aataataggc cgctttcggt aaaatttcgc taaccggcaa
     8461 catcaccagc ccctgtccgt gcaagggatc gcagcccatt tcttcaggca gtatgctgag
     8521 aaaatcgctt tttgccacca gactgataca ggctgagaac gtctcgcaga ctacaccgac
     8581 ctgtggcgtt tgcgcctgat cgtcaagcaa ttcactcaac tgtttgtagt aactgccgtg
     8641 tggcgtcggc attgtccagc tgtaatccag taactgtttg atcgaacggg caccaatggc
     8701 ggggtgtccc gggcggcaaa agatcgcgaa ttgcttttcc agtaatttct caaaagtaaa
     8761 ttcgtggtcg tacggtccct gataataggt attgatggtg aaatccaatt ctccctgacg
     8821 caattcatta atcatcgaca ccagttgccc ttccataatg cgtactttta cctgcggatg
     8881 ctgctgatga aaacgagata tgacagctgg catcagactg cgggaaatac tggcccccat
     8941 gccgatatta atctgccctg ccagttgccc ttgtcgttgg cgaatttcct cttgcgctgc
     9001 gcgcagctct tcaagaataa gactggcgtg ctgataaaaa ctttcaccgg catcagttaa
     9061 cgtcacgcct ttactacggc gaaaaaagag ttgcgccgct aatccttctt ccagctcctg
     9121 aatagattta ctcagtgccg gttgcgacat attcaacatt cggctcgctc cgcgaatgct
     9181 gccctgacga gccacttcaa caaaagcacg aatttgatga atttttacct gaaaagccat
     9241 gacgccaccg ataaccgtta tttatcagac caaagaaact ggcatctact ttaatgcaga
     9301 tgattgtgtc agggtaattt atgaacggtt aaaactgtga aaaatcagtt agtgataagt
     9361 aaaaactatc gctacgtgaa ccgggtcaca ctttttactg atgacgggaa aggttatgga
     9421 gtctttgaat caatttgtta attcgcttgc cccaaaatta tcgcactggc gacgtgattt
     9481 tcatcactat gcagagtctg gctgggtgga attccgcact gccacccttg ttgcggaaga
     9541 attgcagcag ctcggctatt cactggcgct gggccgcgaa gtagttaatg aaagtagccg
     9601 gatgggacta cctgatgaac tcactctaca acgcgaattc gagcgcgctc gtcaacaggg
     9661 tgcgctagca caatggattg cggtttttga aggtggtttc actggtatcg tcgccaccct
     9721 ggataccggt cgccccggtc cggtgatggc tttccgtgtc gatatggacg cgctggatct
     9781 cagtgaagag caggatgtca gccatcgccc ctaccgcgac ggttttgcgt catgtaacgc
     9841 cggaatgatg catgcctgtg gtcatgatgg acataccgcc attgggcttg ggctggcgca
     9901 tacccttaaa caattcgagt ccggactaca tggcgtcatc aaactgattt ttcagcctgc
     9961 agaggaaggt acgcgtggcg cgcgggcgat ggtcgatgca ggtgtcgtag atgatgttga
    10021 ttattttact gccgtgcaca ttggcactgg cgtacctgcg ggcaccgtgg tgtgcggcag
    10081 tgataatttt atggcaacca ccaaatttga cgcgcacttc accgggaccg ccgctcacgc
    10141 aggcgcaaaa ccagaagacg gtcacaatgc cttgttggcg gcagcacaag caactcttgc
    10201 actgcatgca atcgccccgc acagcgaagg agcttccaga gtaaacgtgg gcgttatgca
    10261 ggcaggaagc ggccgtaacg ttgttcctgc ctcggcgttg ctgaaagtgg aaacacgcgg
    10321 ggccagcgac gtcattaatc aatatgtttt tgaccgtgca caacaagcga ttcagggcgc
    10381 agcaaccatg tatggtgtcg gcgttgaaac tcgtctgatg ggtgcagcta ccgccagttc
    10441 tccttcgccg caatgggtcg catggttgca aagtcaggcg gctcaggtcg cgggggtcaa
    10501 tcaggccatt gaacgtgttg aagcgcctgc gggttccgaa gatgccacat taatgatggc
    10561 ccgcgtgcag caacatcaag ggcaagcctc ctacgtggtg tttggcacac agctggcggc
    10621 aggtcatcac aacgaaaaat tcgattttga cgagcaggtt ctcgctattg ccgtcgaaac
    10681 gctggcgcgc accgcgctca attttccctg gacgcgaggt atctgatgca ggaaatctat
    10741 cgttttatcg acgatgcgat tgaagccgat cgccaacgtt ataccgatat tgccgatcaa
    10801 atctgggatc atccagaaac acgttttgaa gagttctggt cagcggagca tctggcttcg
    10861 gcgctggaat ctgcaagctt caccgttacc cgcaacgtag gcaatatccc aaatgcattt
    10921 attgcttcgt ttggtcaagg caaaccggtt atcgccctgc tgggagaata tgacgccctg
    10981 gcaggtttaa gtcagcaagc tggttgcgcg caacctacat ccgcgacgcc cggtgaaaat
    11041 ggtcacggtt gcggacacaa tttgctggga accgccgcct ttgccgctgc aatagccgtc
    11101 aagaaatggc tggaacaata tgggcaaggc ggcacagtgc gcttttatgg ttgtcctggc
    11161 gaagaaggcg gctcgggtaa aacgttcatg gtccgcgagg gggtatttga tgatgtggat
    11221 gcggcactca cctggcaccc ggaagccttt gccggcatgt tcaatacccg cacgctggca
    11281 aacattcagg catcatggtg ctttaaaggg atcgcagcac atgccgcgaa ttcccctcat
    11341 ttgggacgca gcgcccttga tgccgtaacg ttgatgacca ctggcaccaa cttcctcaac
    11401 gaacatatta ttgaaaaagc gcgcgtacac tatgccatca caaatagcgg cgggatctcg
    11461 cccaacgtgg tccaggcgca ggcagaagtg ctttatctta tccgcgcccc cgaaatgacc
    11521 gacgtgcagc atatttatga tcgggtcgcc aaaatcgccg aaggtgcggc attgatgacc
    11581 gaaaccacgg ttgaatgccg cttcgacaaa gcctgttcca gttatctccc gaatcgcacc
    11641 ttagaaaatg ccatgtacca tgccctatcc cattttggta ccccggaatg gaactccgaa
    11701 gaactggctt ttgcgaaaca aattcaggct acgctcaccc ccaacgatcg gcaaaacagt
    11761 ctgaataata tcgccgcaac cggtggcgaa aacggcaagg cttttgcact acgtcatcgt
    11821 gaaacggtac tggcgaatga ggtcgctcca tatgccgcca ccgataacgt gcttgcggca
    11881 tcgactgatg tcggcgacgt cagttggaaa ctgcctgttg cccagtgttt cagcccctgt
    11941 tttgccgtcg gtacaccgct acatacgtgg caactggtta gccaggggcg aacatctatt
    12001 gctcataaag gaatgctgct ggcggcgaaa actatggcag caaccacagt caatctcttc
    12061 attgattcag ggctattgca agaatgccaa caagagcatc agcaagttac ggacacgcaa
    12121 ccgtatcact gccctatccc gaaaaacgtg acaccgtcac ctttaaaata acaacaacaa
    12181 cgcaaacaca acaaccgagg aatgcccatg agtatgtcat ccataccgtc gtcctcccaa
    12241 tccgggaagc tctatggctg ggtcgaaaga attggtaaca aggttcccca tccttttttg
    12301 ctctttatct atttgataat cgtactcatg gtgacgacgg caattttgtc agcctttggc
    12361 gtcagtgcga aaaacccgac cgatggtacg ccggtggtgg tgaaaaacct gctcagtgta
    12421 gaaggattac actggttttt acccaatgtg attaaaaact ttagcggttt tgctccactt
    12481 ggagcgatcc tggcgctggt tttaggtgcc ggcctagcgg agcgcgtcgg cttactgcca
    12541 gcgctaatgg ttaaaatggc atcgcatgtt aatgcccgct acgccagtta tatggtgctg
    12601 tttattgctt ttttcagcca catttcttcc gatgcggcgt tagtgatcat gccaccgatg
    12661 ggtgcgctga tttttctggc ggtgggcagg catccagttg caggtttact ggccgccatt
    12721 gcaggcgtag gttgcggctt cacggctaat ttactgattg tcacaaccga cgtgttgctg
    12781 tcggggatca gcacggaagc ggcggctgcg ttcaatccgc aaatgcacgt cagtgtaatt
    12841 gataactggt attttatggc cagctccgta gtcgtactga cgattgttgg cggcctgata
    12901 accgacaaaa tcatcgagcc acggttaggt caatggcagg gaaacagcga tgagaaactg
    12961 cagacattga ccgaaagtca gcgttttggt ttacgcatag caggtgtcgt atcgctactt
    13021 tttattgctg cgatcgcgct gatggtgatc ccggaaaacg ggatattgcg cgatccgatt
    13081 aatcacaccg tgatgccatc accctttatt aaaggtatcg tgccactgat cattcttttt
    13141 ttctttgttg tctcgctggc ttatggcatc gctacccgca caattcgacg tcaggcggat
    13201 ttaccgcatt taatgattga accgatgaaa gagatggcgg gatttatcgt gatggttttt
    13261 cccctagccc agtttgtcgc catgtttaac tggagcaaca tggggaaatt catcgccgtg
    13321 gggctgaccg atatacttga aagttcaggg cttagcggca tcccggcgtt tgtcggtctg
    13381 gcgttgcttt cctctttctt atgcatgttt attgccagcg gttccgcaat ctggtcgatt
    13441 ctggccccca ttttcgtacc aatgtttatg ctacttggct ttcacccggc atttgcgcaa
    13501 atcctctttc gtattgccga ctcatccgta ttgcctttag cgccggtatc tccttttgtt
    13561 ccactatttc ttggattcct gcaacgctac aaaccagacg cgaaactggg tacttactat
    13621 tcgttagtct tgccctatcc gcttatcttt ttggtggtat ggctgttgat gttgctggcg
    13681 tggtatcttg ttggtctgcc gataggtccg gggatttacc cacgtttgtc ttaagagaga
    13741 acggatgctg agattacttg aagaaaaaat tgccacgcca ctgggtccac tgtgggtgat
    13801 ttgcgatgag caatttcgcc tgcgggcggt tgaatgggaa gagtacagcg aacgcatggt
    13861 gcagctgctg gacatccatt atcgcaaaga aggctatgag cgcatttctg ccaccaaccc
    13921 aggcggttta agcgacaagc ttcgtgatta ttttgccggt aatcttagca ttattgatac
    13981 gcttcccact gccacagggg ggacgccatt tcagcgcgaa gtctggaaaa ctctacgcac
    14041 tatcccctgc gggcaggtaa tgcattacgg ccaactggct gaacaattgg gccgccctgg
    14101 cgcggcgcgt gccgttggtg cggcaaacgg atcgaatccc atcagcatcg tcgtaccttg
    14161 ccatcgggtt attggccgaa acggcaccat gaccggatat gcaggcggag ttcagcgaaa
    14221 agagtggtta ttgcgccacg aaggttatct tttgctgtaa acattaaaca atttgtgcca
    14281 gcttgttcac acttttatgt aaagataccc ttaacaactt aagggttttc aaatagatag
    14341 acatatattt acatctaata tcggaattct ctgctgttaa ggtttgctta gacttacttg
    14401 ctccctaaaa agatgttaaa attgacaaat atcaattacg gcttgagcag acctatgatc
    14461 ccggaaaagc gaattatacg gcgcattcag tctggcggtt gtgctatcca ttgccaggat
    14521 tgcagcatca gccagctttg catcccgttc acactcaacg aacatgagct tgatcagctt
    14581 gataatatca ttgagcggaa gaagcctatt cagaaaggcc agacgctgtt taaggctggt
    14641 gatgaactta aatcgcttta tgccatccgc tccggtacga ttaaaagtta taccatcact
    14701 gagcaaggcg acgagcaaat cactggtttc catttagcag gcgacctggt gggatttgac
    14761 gccatcggca gcggccatca cccgagcttc gcgcaggcgc tggaaacctc gatggtatgt
    14821 gaaatcccgt tcgaaacgct ggacgatttg tctggtaaaa tgccgaatct gcgtcagcag
    14881 atgatgcgtc tgatgagcgg tgaaatcaaa ggcgatcagg acatgatcct gctgttgtcg
    14941 aagaaaaatg ccgaggaacg tctggctgca ttcatctaca acctgtcccg tcgttttgcc
    15001 caacgcggct tctcccctcg tgaattccgc ctgacgatga ctcgtggcga tatcggtaac
    15061 tatctgggcc tgacggtaga aaccatcagc cgtctgctgg gtcgcttcca gaaaagcggc
    15121 atgctggcag tcaaaggtaa atacatcacc atcgaaaata acgatgcgct ggcccagctt
    15181 gctggtcata cgcgtaacgt tgcctgattt ttccgcataa ctcactatcc ttctgtcata
    15241 tcattaaatt tttctgattt attgatctgg cagaaggttc atcactgttt cattcaccag
    15301 atatgggtta atcttttaat tacaaactgc gttgacagtt gttgtaagga gaccctgtat
    15361 ggctatgtat cagaacatgc tcgttgttat cgatcctaac caggacgacc aaccagcatt
    15421 gcggcgagct gtttatttac atcaacggat tggtggcaaa attaaagcct ttttgccgat
    15481 ctatgacttc tcatacgaaa tgaccaccct gctctccccg gacgaacgta ccgctatgcg
    15541 tcagggcgtc atcagccagc gtacagcctg gatccacgag caggcaaaat attatctcaa
    15601 tgctggcgtt cccattgaaa ttaaagtggt ctggcataac cgtcccttcg aagccatcat
    15661 tcaggaagtg atcagcggcg ggcacgattt ggtgctaaaa atggcgcacc aacatgaccg
    15721 tctggaagcg gtgattttta cgccaacgga ctggcatctg ttacgcaaat gcccaagccc
    15781 ggtgtggatg gtgaaagacc agccgtggcc ggaaggaggc aaggcgctgg tggcggtgaa
    15841 tctcgccagt gaagagccgt atcataatgc gctcaatgaa aaactggtca aagagacgat
    15901 cgaactggca gaacaagtca accataccga agttcatctg gttggcgctt atccggtaac
    15961 gccaattaat atcgcgattg aactgccgga atttgatccg agcgtttata acgatgccat
    16021 tcgtgggcaa catttgctgg caatgaaagc tctgcggcag aaattcggca ttaatgaaaa
    16081 catgacgcac gtagaaaaag gtctgccaga agaggtgatt cctgatttgg cggagcactt
    16141 acaggcgggg attgtggttc tgggcacggt aggacgcacc ggtatttcag cagcattcct
    16201 cggcaacacg gcggaacagg tgattgatca tcttcgctgc gatctgttgg tcattaaacc
    16261 tgaccagtat cagacacccg ttgaactgga tgacgaagaa gacgattaac gactcttcaa
    16321 taactgacaa ggcccgagtt atcgggccta ttttttactt actgctcttc gccgccaagg
    16381 aaataaattc ccaacgggat tgccagcaac acagtaaaga ccaggctata aacaaaaatc
    16441 atcgatgact gaatcacata cattgatgtc gtccactcag acagcggaat attgtactgt
    16501 tctataacgc cgccgatagt cgcgcgagtc acaaccgacg ccgcaataat gaaaaccgcc
    16561 aacaaaccaa agagaaattt cttccctttc gctgacttca gtttcgccat aatcatgatg
    16621 taatccttgt gcagacaaat ttcttgttac ctaaacgtaa caataccatg acatttcacc
    16681 gttgtctgct ctgaaaacct cacctcttag ctggatttac aatcaacgca ttgattagtt
    16741 agctaatgtt gctacttcct tccctgacta ttgatgatct gaatgaattg tcgcggtaaa
    16801 atagccccgc caggggtttc cggcatgacg acaattatct ggaggagcct gataaaacaa
    16861 tgatcgctga actgtttaca aataatgcgc ttaatctggt cattattttc ggtagctgcg
    16921 cagcattgat tctgatgagc ttttggtttc gccgcggaaa tcgtaaaaga aaaggttttt
    16981 tattccatgc ggtgcaattt ttaatctaca ccataattat cagtgctgtt ggtagcatta
    17041 ttaattatgt tatagaaaac tataaactca aatttatcac cccaggcgtt atcgatttta
    17101 tctgtacgtc cctgattgcg gttattctga cgattaagct atttttgctg attaatcagt
    17161 ttgaaaaaca gcagattaaa aaaggtcgcg atatcaccag tgcgcggatt atgtcgcgca
    17221 ttatcaaaat caccattatt gtggtgcttg ttctgcttta tggcgaacat ttcggcatga
    17281 gcctttctgg cttgctgacc tttggtggta ttggtggtct ggctgtcggt atggccggta
    17341 aagatattct gagtaatttc ttttccggga tcatgctcta tttcgaccgt cctttcagta
    17401 ttggcgactg gatccgttca ccggacagaa atatcgaagg tacagtagcg gaaattggct
    17461 ggcgaattac caaaattacg acctttgata atcgtccatt gtacgtaccg aactcgctgt
    17521 tttcgtcgat cagcgtagaa aacccaggac gaatgaccaa ccgccgcatt accacgacca
    17581 ttggtttacg ttatgaggat gcggcaaaag tgggcgttat tgtcgaagcc gtacgtgaga
    17641 tgctgaaaaa tcacccggcc atcgaccagc gacaaacctt actggtttat ttcaaccagt
    17701 ttgccgactc gtcattgaat attatggttt attgctttac caaaaccacg gtatgggctg
    17761 aatggcttgc tgcacagcaa gacgtttatt tgaagattat cgatattgta cagtcacacg
    17821 gcgcggactt tgccttcccc agccagacgc tgtatatgga taacattaca ccgccggaac
    17881 agggtcgcta aatcagacat cactggcgtt atccacaagg gtaacgccag ttcccatcac
    17941 atcaatgctt cacaatatac atagtccgac tgtacgccac atcttcaggg ttattaatgg
    18001 gataaccttt cagccacggt ttgattaatc gtccattggt atattgataa attggtgcaa
    18061 tcggtgcttg ctccatgagg attttttcta ccgcgttgta atcggcatta cgcgctttaa
    18121 cggtattttc cgtcgatgcc tgggccagaa ctttgtcata tgccggattg ttaaagcgtg
    18181 aaatatttcc tgaatgcgtt gacgttaata atgtcaggaa agtggagggt tcattataat
    18241 cccccaccca tgaggcgcgg ataacatcaa aattgccggt gttacggcta tcgatatagg
    18301 ttttccattc ctgattttgc aatttaacat ctacgccaag gttctttttc cacatcgatg
    18361 ctacagcaat cgcaattttt tgatggtttt ctgaagtgtt atacaaaagc gtcagcttca
    18421 gcggtttttg cggaccataa ccagctgcgc tcaacaaagt ttttgcctgc gcattcagtt
    18481 cttcctgact catttgttca aacggcgaag gttccggcgt aaatccggcg gtaacatctg
    18541 gtgtaaaatg ccacgctggc ttttcgcccg tccctaatac tttttcggtc atcaggcggc
    18601 gatctatcgt catacttaat gccagacgaa cgcgctgatc tgccgtcggc cctttttgcg
    18661 tgttaaacgc ataataatag gtcccgagct gcggcggcgt ataaacctgc cccggaatat
    18721 ccttcaacag cttctgatac atatttttcg ggaaggattc ggtgatatca atatcccccg
    18781 cgaggtaacg cttagtggct gcggattcct gattaattgg caggaaggtc actttttgca
    18841 gtaccgtttt ggcgttatcc caataatggg tattcggtac cacgaccagt ttttcattga
    18901 ctacgcgctc tttaagaaca taagcgccat tgccaatcag atttccgggt ttcgtccact
    18961 ctttaccgct ttctacgttg gctttttgca ccgggaagaa ggcaaagtta gcggttaaat
    19021 tcacaaacca cggcaacggt ttatcaagct gaattttcaa agtatgggca tcaactgcgg
    19081 tgacgccaag ctggtcaggc gtagctttac catcaataat cgcctgtgcg ttgttgattc
    19141 ccgccagcgc ggcaaaccat gcaaatggcg atagagtttt cgggtccacc agacgttgcc
    19201 agctgtagac aaaatcttgc gccgttaccg gtgtgccatc cgcccatttt gcgttatcgc
    19261 gcagggtaaa agtccagata cggttgtcat tacttttcca ctgagtcgca acgccgggga
    19321 caatctcccc tttctcgttc tgattcacca gtccttcaaa cagatcgcga atgacctgaa
    19381 tctctggcag gcccacggct ttagcgggat ccagcgacgc aggctcatct ttaatgtggc
    19441 gcaccagctc ctgcttctct gccagtactg tgccgctcgg aacttctgca gcatacgaaa
    19501 gagaaatgct gctgaccaac agcgcacaac acgtgactga aacagagtgc ttcataagtt
    19561 tccctctaac cgacattaaa attgatgcgt aattatttgt ttcgccttaa agaaatgcaa
    19621 acaacttcca tcaaaaaagt
//
LOCUS       DAPHJE010000086        18822 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPHJE010000086 DAPHJE010000000
VERSION     DAPHJE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.086"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..907
                     /locus_tag="RH222_003104"
     CDS             <1..907
                     /locus_tag="RH222_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3798090.1"
                     /translation="LTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRA
                     GFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDT
                     FQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAP
                     EQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEE
                     LAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQF
                     WEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(1024..2367)
                     /gene="gdhA"
                     /locus_tag="RH222_003105"
     CDS             complement(1024..2367)
                     /gene="gdhA"
                     /locus_tag="RH222_003105"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV3798091.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2603..2875
                     /locus_tag="RH222_003106"
     CDS             2603..2875
                     /locus_tag="RH222_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDV3798092.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(2841..3248)
                     /gene="nudG"
                     /locus_tag="RH222_003107"
     CDS             complement(2841..3248)
                     /gene="nudG"
                     /locus_tag="RH222_003107"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV3798093.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLESFMALRAARPAD"
     gene            3335..3955
                     /locus_tag="RH222_003108"
     CDS             3335..3955
                     /locus_tag="RH222_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV3798094.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            complement(3964..5271)
                     /gene="ynjE"
                     /locus_tag="RH222_003109"
     CDS             complement(3964..5271)
                     /gene="ynjE"
                     /locus_tag="RH222_003109"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV3798095.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(5338..5991)
                     /locus_tag="RH222_003110"
     CDS             complement(5338..5991)
                     /locus_tag="RH222_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV3798096.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5991..7526)
                     /locus_tag="RH222_003111"
     CDS             complement(5991..7526)
                     /locus_tag="RH222_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDV3798097.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(7499..8665)
                     /locus_tag="RH222_003112"
     CDS             complement(7499..8665)
                     /locus_tag="RH222_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3798098.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEAKLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(8675..9223)
                     /locus_tag="RH222_003113"
     CDS             complement(8675..9223)
                     /locus_tag="RH222_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV3798099.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(9223..9930)
                     /locus_tag="RH222_003114"
     CDS             complement(9223..9930)
                     /locus_tag="RH222_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV3798100.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(9945..10622)
                     /gene="ydjY"
                     /locus_tag="RH222_003115"
     CDS             complement(9945..10622)
                     /gene="ydjY"
                     /locus_tag="RH222_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDV3798101.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(10627..11337)
                     /locus_tag="RH222_003116"
     CDS             complement(10627..11337)
                     /locus_tag="RH222_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV3798102.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(11504..12310)
                     /gene="xthA"
                     /locus_tag="RH222_003117"
     CDS             complement(11504..12310)
                     /gene="xthA"
                     /locus_tag="RH222_003117"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV3798103.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12756..13976
                     /gene="astC"
                     /locus_tag="RH222_003118"
     CDS             12756..13976
                     /gene="astC"
                     /locus_tag="RH222_003118"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV3798104.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTSNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            13973..15007
                     /gene="astA"
                     /locus_tag="RH222_003119"
     CDS             13973..15007
                     /gene="astA"
                     /locus_tag="RH222_003119"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV3798105.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            15004..16482
                     /gene="astD"
                     /locus_tag="RH222_003120"
     CDS             15004..16482
                     /gene="astD"
                     /locus_tag="RH222_003120"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV3798106.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            16479..17822
                     /gene="astB"
                     /locus_tag="RH222_003121"
     CDS             16479..17822
                     /gene="astB"
                     /locus_tag="RH222_003121"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV3798107.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            17815..18783
                     /gene="astE"
                     /locus_tag="RH222_003122"
     CDS             17815..18783
                     /gene="astE"
                     /locus_tag="RH222_003122"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV3798108.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         4436 a         4864 c         5094 g         4428 t
ORIGIN      
        1 tttaacatgt gttgtcgctg taatgtttgt attcgcagcg ataacatcca acggtttgaa
       61 tgaaagaggc ctgattactg cgggatgctc ttttctttat ctattaatta tgatggggct
      121 tattgttcgg gccggtttta aagcaaaaaa agaacaactg cattattatc aggctaaagg
      181 tattgagccg ctcagtatcg agaagttaca ggcgctacaa ttgatcgcac cttatcggtt
      241 ctatcataag caatggtctg aaacgctgga gttctggccg cgaaagcctg aacctggcaa
      301 agataccttc caatatcatg tgcttccctt tgactcgatc gatatcataa gtaaaagacg
      361 cgagtcttta gaggatcaat ggggtatcga agatagcgaa agttattgtg ccttaatgga
      421 gcattttctt tctggcgacc atggagccaa tacctttaaa gcaaacatgg aggaagcccc
      481 agagcaggtt atcgccttgt tgaataaatt tgctgttttt ccctcagact atatctctga
      541 ttgcgctaat aatcgctccg gtaaatcctc ggcgaagcta atatgggcgg cggaattatc
      601 atggatgacc tcgatatcaa gcacagcttt tcaaaacggg acaattgaag aagaactggc
      661 ctggcattat ataatgcttg cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga
      721 ttatcaaaaa aatagtctaa tgggatttct ttactggcat atctgctgct atcgcagaaa
      781 gttaacggat gctgaacttg aagcgtgtta tcgttacgac aagcagtttt gggagcacta
      841 cagtaagaaa tgccgttggc ccataagaaa tgttccctgg ggagcatcat ccgttaaata
      901 ctcataaacg cctgaaattt tgccgggggc gctgcgcttg cccggcctac attagagcgt
      961 aactgcaatg aattgtgccc atttgtaggc ctgataagcg tagcgccatc aggcatttac
     1021 aacttaaatc acaccctgcg ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt
     1081 cgcgccctgc acgtagttgg tttgctcacc ttcaccacca tgctcaacac aggcatggtg
     1141 gatatccagc atgatgtgat gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg
     1201 cgcagcgttt tgcgccattt caaggcctga cgttgctaca ccgccagcat tagccgcttt
     1261 acccggtgca aacagtacgc ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg
     1321 catatttgcc ccttcggcga cggctttaac gccattggcg ataagctgat gcgcggcgtc
     1381 aacatccagt tcgttctggg tggcgcaagg cagggcgata tcaaccggta cagaccacgg
     1441 ctgttggcgt tcgagataga ccagaccaaa ttctttggca taatctgcca ctcgaccatc
     1501 gcggctggct ttgatttcga taagacgtgc cagtttctct ttcgtgaatc cgctttcatc
     1561 aactacagtg ccgctggagt ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt
     1621 ttcgatagcg tactgggcga cgttgccgga gccagaaacg gaaacgcgca tcccttcaaa
     1681 acccataccg tggcgtttta gcattgcttc tgtgaaataa accagaccgt agccggtagc
     1741 ttccgggcga ataagactgc cgccaaatga aaggccctta ccggtgaaga cgcaggcggt
     1801 attgttggag agctttttca tcatccccgc cataaagccg acttcacgac caccaacccc
     1861 gatatcacct gccggaacgt cggtatccgc gcccaggtgg cgatacagtt cagtcatcag
     1921 cgcctggcaa aaacgcatca cttcaccttc gctttttcct ttcggatcga aatcgctgcc
     1981 gcctttacca ccgcccatcg gcagggtagt cagggcattt ttgaaggttt gttcgaagcc
     2041 gaggaattta agaatggaga ggttaactga cggatggaag cgcataccgc ctttgtacgg
     2101 gccgatggca gagctgaact gcacacgcca tgcacggttg acctgtacct gattgcgatc
     2161 atcaacccat accacgcgaa actggatcac gcgctccggt tcaaccagac gctccagtaa
     2221 tgacatctgg cgatattttg gattttgttc aagaaaaggc cagagtgtgg tcattacttc
     2281 acgaacggct tgcgcgaact cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa
     2341 tgactccaga gaatatgtct gatccataga tataaaaccc ttatatatta atacgattgt
     2401 gcttatgttg tttatgtgat gtgcttttgc agttttcgac tataccatca agaatgtgac
     2461 ggtaagcaag aaaaaaatga cccaggaaag caaattaata acgagagtaa tctcataacc
     2521 aaatgcgata actcagacgc taaagttttg ccacccggct accgttatca gtagcagaga
     2581 caataacgtt aaggagttcc ctgtgagtcg agcattgttc gccgtagtat tagcatttcc
     2641 attaattgca ctggctaacc cgcattatcg tccggacgtg gaggtaaacg taccgccaga
     2701 agtgttcagt tcaggcgggc agtcagcgca gccctgtact cagtgctgcg tttatcagga
     2761 taaaaattat tctgaaggcg cggtaattaa agccgaggga atactgctgc aatgccagcg
     2821 ggatgacaaa acccttagca ctaatccgct ggtctggcgg cgcgtaaagc cataaacgac
     2881 tctaataatg gaatgtcagc aggggccagc ggatattgca gcgcctcttc aggtgagcac
     2941 cagaccagcg cctgatgttc atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag
     3001 gcatgaagat ggataatccg gcccgaaact tctcgctgat ggctggtaac atactcaccc
     3061 acagttgctt cgatgcccag ttcttcgcgt aactcacgca ccagcgcctg ccgctggctt
     3121 tcatccggct cgactttacc accggcaaac tcccataatc ccgcctgatc gctctgggcg
     3181 gggcgttgcg cgagtaaaat tttgccatca cgttcaatga tggcggcaac aacttcaatc
     3241 attttcataa attacacacg ggcagataag cgaaaaatgc cataatatcc gcactgttgc
     3301 tcaatcagaa aggctttata tttaaggagt gcctgtgcta gaccgccatc ttcatccccg
     3361 gattaaaccg ttgttgcatc agtgcgtgcg ggttcttgat aaaccgggca ttacgccaga
     3421 tggtttaaca ttagtgggat ttgccatcgg cgtgctggca ctgccttttc tggcgctggg
     3481 ctggtatctg gcggcgcttg tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg
     3541 tgcgctggcg cggcgcagag ggcttaccga cgcgggcggc tttcttgata tttctctcga
     3601 ttttcttttt tacgcgctgg taccttttgg gtttatcctc gccgcaccgc agcaaaacgc
     3661 cctggcaggc ggctggctgc tgtttgcgtt tatcggcaca ggcagcagtt ttctcgcttt
     3721 tgctgcgctg gcggcgaaac atcagattga taatcccggt tatgcgcata aatcgtttta
     3781 ttatctgggc gggttaaccg aaggcacaga aacgatctta ctgtttgtgc tgggatgttt
     3841 attccctgca tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac
     3901 ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg caccagcgtc agtaattgca
     3961 atgttatttg ctactgtccg ggccgcgttc accggttgct accggatttt ttggatcgct
     4021 gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc
     4081 gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgctc
     4141 tggtttgata ttccacgctt tccacatagc ggtaatatca tcggcgctgc gcatggtgcc
     4201 atccgggtta tgaaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc
     4261 tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc
     4321 tggccacgaa cgaatgctta ccagcgatgc atcctggcga tgcagcagtc cacgggcctg
     4381 ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat ccggttccgc
     4441 tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca
     4501 gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg
     4561 cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg
     4621 ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc
     4681 cacttcgttg gtatcgatgt agtcagcacc gggaatatgg ctgataaggt aaagcttagg
     4741 agcgccccag gccgcttcaa tgactttcca gtcaccggca ggtttcgccg taacctcttt
     4801 accttgttgc aggtcgtgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt
     4861 ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc
     4921 tttttgcagt cgcgttttga cggcgtcgac atctttgtca ttaccgtaca gcgccaccgg
     4981 ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt
     5041 gtcgagccag ctggcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa
     5101 ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg
     5161 ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca
     5221 agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc
     5281 cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta
     5341 tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg
     5401 aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagttcgc gaacttcgct
     5461 gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc
     5521 caggagtaac gcttttggct gagcgagaag ggcgcgcagc agggccaccc gcgctcgctg
     5581 accgcctgat aatgtcgcag ggtcctggtg ataagtttca gccagtcccg ctcgatcaag
     5641 cgcgtctttc acggcattgc gcctggcagt ccccttgagc gtcgacggta gcgccagcag
     5701 taaattttgc ccgacgctga actggtcaaa taacagtgca tcctggaaaa gaatgccaat
     5761 ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt
     5821 acaagaaaac tgtccagcca gtgcaccaat catccatgaa aacagagtgg attttccaca
     5881 gccagacggc cccattaacg tgacaatgtc acctttatcc accgtaaagt taacgtttgt
     5941 cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggagt
     6001 ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc
     6061 ggtaatagca gttgccataa agcctgggcg gcgaaaatac cattgctgcc gccgctgctt
     6121 aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc
     6181 atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcgt agggcgtagc
     6241 ataagcggac atttcacgta aaagaagatt ttggcccgcg accagctcag tgtttgcgca
     6301 atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac
     6361 ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc
     6421 catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag aatgggcaac
     6481 cagagccaca actggcgacg ctgtggcccc cattccagcc acagcagtaa caggagcaaa
     6541 gcgatgaatg tcgccaccag ccccattgtc aggctgttga tgagtgcttc actattgatc
     6601 gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa
     6661 aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg
     6721 cggggaatag tgcgccgcca gctgcgccat agcagatagc tcagcaaaac gtaggcggcg
     6781 agtaacagca tcagcagcag actggcgagt gcgcctttcg tttgttgatc cgcgtcgccc
     6841 tgagttaacc actgccagct aattactgcc agcgtcggtg gattacctgg cccaagaata
     6901 atcgccacat ccacgaccga aagcgaccag gcaacaatcg ccaacatcgc cattgccagt
     6961 gcaggtgcga cggagggcaa gagcagccag ttcaggcatt gccagcggct gtagcccagc
     7021 gaatccagca caatgacctg ctgcaacagc cgtttttcgc tcaatactgc cgctaagatc
     7081 cacagcagaa atgcgctttc tttcactgca agggtgaggc ccagcccaat acccaatttg
     7141 tccattagcg gagtgaaata cgggaaatag tcataaagca gccctccgtc agcaaagagc
     7201 agaagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg cagacgggca
     7261 cacatacgct gccatttcgg ccaaggccac agcgccacaa tcaccagcag ggcaatcaac
     7321 aacgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc ctgcggtaac
     7381 tgtggatcgg caaataacgc ctgccagtgt gtcaacgaca aagcaggcga tatcagcgtg
     7441 agagctgccg ggatcagtgg tgcataaatc accgccacca tcgcccacag caaaaagatt
     7501 aatgcgtacc gtaacggtgt agccattctt gttccagcgc atttacccaa cctgcgtgcg
     7561 gttcagccag taccggcggc agatcttgcg gcattcttga ttgcaatgtt tcgcgctgcc
     7621 catcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacagcg ggatccgctt
     7681 tacgcagttg cgcattgggt gagagcagga agttggcaac caccttcgcc gcagcactgg
     7741 catttgcgtt agcaggaatg gtgacgaaat gcacgttgcc aatcatcccc tggttaaaac
     7801 caaaactgta actgcttgca ggcaaatcgc cgctggcgat tttttgctgg gcatgagcag
     7861 ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc atccgcgcgg
     7921 gcgaaggcgg gaaatccttt cctttgcgcc ataaataggg atgtagcgcg tcaagatatt
     7981 gccacaaggg cgcagtgacg cgggcgaaag tggctttatc cggggcaact tttaacgcgg
     8041 cgggatcggg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg gtaaagtccg
     8101 gtgggcgcgg ataggtaacc gtgccgggat tggctttagc aaactccagt aaggcttgcg
     8161 gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt tgtgcgccgc
     8221 cccagggcga ttcagcccct tctgtaggca ctgaaaaatc ttcccgcacc ggcagctgtg
     8281 tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat aacttggcct
     8341 cttttaaggt gcggaagttt tcgccgttca cccagagcag atccaccgag ccgcccgttt
     8401 tacgaccggc agcagcttcg gtctgaatgc gcttcaccgc gtctgcggca tccgcgagac
     8461 gaacaatctt caggtttata gcgtaatggg ttttcatctc gccgctaacc cagtcgagat
     8521 agcggttaat tgcggtatcg ccgccccagg cgttaaacca gacggtttgc cctttggcct
     8581 catttttaat ttcctgccag tcactggcat gtgttgccaa agaaaacagc gataacaatc
     8641 ccagcaacca cccacaatgg cgcatcatct ctccttaaga acgtttttca ggaatacggc
     8701 acagcccctg agcgggaatg tccatggcag aattaaaacg agccgacagg ttttgcaggc
     8761 cgatcaatgc cgtcagttcg gtgagcgcct gagcgtcaaa atgcgcagcc agtcgggtac
     8821 gcagggcatc atcgactgtt ggcggtgtga cgcttgcggc ttcggcgtac tccagcgcca
     8881 gccgttcttc atcgctaaag agcgggcttt gccgccagtc agccactgcc agcagtttat
     8941 cggtgctgcc ggtacgctcg gcgactttca tactggtgat atccacgcaa aactcacaca
     9001 ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgcac taccggatcg agcggtgagc
     9061 gtttgcgctc cagccaccca acaaacattg acaccaaata aaagataaac gggatccgcc
     9121 cccaccagcg aatcgggctt agcacctcgc cgtaatggcg acgatgcagc cacgcctgcg
     9181 gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcaggcat tcctcttctg
     9241 gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa acagaataaa
     9301 cagtccggtg acaaaccaga aagtaccgcc tgttaacatg ctgcccgccc aggaataaac
     9361 aatagtcgcc ggtaattgac caagcccggt ggcgataaaa aacgagcgaa aacgtattga
     9421 agtcagaccg gcagcatagc tgattggatc gaaagggaca aaaggcaata accgacagac
     9481 caggatggtg tgtttgccgt agcgagtgaa aaagccgtcc atactgtcaa gcacggtttt
     9541 gccggttaat ttttccacca cttcgcggcc catcactctg gcgataaaaa agcacagcgc
     9601 cgcgccggcc atcgaactgg tccacgacag caagccgccc cagaacgcgc caaacagcga
     9661 cgcattggca aaggtgatca aaaacgcagg tagcggtgca gcaatggcct gtaaaatcat
     9721 caacaagaac gagacaactg ccgccagtgc gccgtaagac tgaataaagc gttctatacc
     9781 ctgttggtcc acggcggcaa acgccgcaac gctgcgattg ataaactcat ggacgccggg
     9841 aagcagcgcc catgcgagca gcatagcgaa caacaggatg atgagggtta tacggtagta
     9901 ccagatttta cgcgattgta tcttcatcat cctttatcct ggctttattc ggtgatttta
     9961 aaggtaaccg ttgccagcgt gttatccgcc gggagaactg aggcattgcc tttgaatttc
    10021 acttcgccgc gtttttccac cgcaccataa gtgtatgttg cattgctgac gatgccgacc
    10081 gggcagctat ccagacacac caggcaaccc gttttcttct cttctgctgc cgttaaatta
    10141 ccgccaaagc gcatgtccag tttcttgcca ttactgtcaa caatcacttc atcgaaggaa
    10201 tacgcttttg ccgccccttg ccagttgacc gaaatatcca gtttgctgcc tgtgacatga
    10261 gtcgtttctt tattatccat cgtcatgttt tcgcccggcg taccacctgc ctctttcagg
    10321 gcttcataaa atgctttcgg ggtcgcataa gccataaaca gcgatttatg tccgttggag
    10381 ccatctttaa acacaatgcc gtgacgagtg tcgtcggtga ggaaacgacc attaatttgc
    10441 accagcatgg ttaccgtatg gttattggca tcgacgcgca gcggttggct gttcgaaagt
    10501 ccgtcgtatt ccacttttgc cgccgcattc tctttttgat cgcagccgct aagcccggca
    10561 acagccagta agcagagtgc agccagtcct tttttccatg acactgaata atgttgcgac
    10621 atcgttctat ccttcatttt ttgggtgagt aagtgggctg cggcgcgaag cagacagatc
    10681 cacatgtttg tgtcgggcgt agagttttgc gagctggacg agaataaaca gcgccaggcc
    10741 cgccagacag agttgtaaaa taaagcgcag cgtaatgcct tcattggcga gatcgcttgc
    10801 catcacggta taaataacaa taccgggcag ggtcgtgagt gccgaaataa gggtataagg
    10861 ccagaaggcg attgtggtta atccgtaagc gtaattttga atattgtaag gaaacaacgg
    10921 gattaagcgg gtcagaataa gaaaatcaat accgttacgc gcaatgcctt tttcaatggc
    10981 ctgaaaggta tggctatgac caacgtattt cagcagtaaa tcacgtccca gccagcgcgc
    11041 cagcaggaat gagcacgagg aggcgagcgt ggcggcaatt aatgagagta gtgtccctaa
    11101 gagcgggcca aaaactattc ctccagcgat caccagtatg cttcctggta acagaaagag
    11161 agtggcaata atgaataaca gaatatagag gctataaccg aacaatccgc tctggcggat
    11221 gagtgtctgt aagtggggta aatcggtgag cagatcgaat aaaccgaaag tgtggatagc
    11281 gtaaatgacc agcgcaaaaa taagacaggc caaaagaaat ttacgctcag cgttcatcgt
    11341 ggagtttcct atatcaaact tgataataga gaagtggtca gacaactaaa aacgcagtga
    11401 cggtataaca aaggacggca ggcaacaaat cattactgac agtttgttga tttttctcaa
    11461 ggttaattct cctgacccag tttgagccag gagagctgct aaattagcgg cggaaggtcg
    11521 cccagacggg ggcgtgatcg gacggttttt ccatgctgcg aatttcatag tcgatgccgg
    11581 tttctacgca acattctgcc agcggttggc tggcgagcag caggtcgatg cgcagaccac
    11641 ggttatcgtc aaaacctttt gagcggtaat caaaccatga gaaacgatct gctgtttgcg
    11701 gattagcatg gcggaaagta tcgaccaacc cccagctcat cagcctgtcc atccattcgc
    11761 gctcttccgg caggaaagag catttaccgg tacgcagcca gcgcttacgg ttttcttcgc
    11821 caatgccgat atccagatct gtagggctga tattcatatc gcccataatc agtaccggat
    11881 tatcacgttt gagttcggtt tccaggtagt tttgcagatt ctgataaaac tgcgcttttg
    11941 ccgggaattt tatcggatgg tcgcggcttt caccctgcgg gaagtaaccg ttgatcacgg
    12001 tgacattacc cagcagtgag gggatttccg ccataataat ccgccgctgc gcctcttcgt
    12061 cgtcaccggg aaagccgcga cgcacggcaa tcggcgtctc tttggtcagc agcgccacgc
    12121 cataatggcc tttctgcccg tgataaaaca cgttgtagcc gagcttcgcc acctcttcga
    12181 gcggaaacat atcgtcatga acttttgtct cctgcaggcc aatcacatcc ggttggtgct
    12241 tttcgacgat ggcttcaagc tggtgaggtc tggcgcgcag gccgttgata ttaaaagaga
    12301 caaatttcat agtcgctgcc atttattcag ataaagagtg cgtggatggt agcagaattt
    12361 cgcgttgctt accgcctgtt gttaagtgat ttgatcgatg gttgttaatg atgtcaacga
    12421 tggcgcaaaa aatgcccgct tttggtgcgc gctgcgtcag aatggcgcag taatttccag
    12481 taaatttcgt caatgatcac aattccagaa ttatatttta cttctgcata acattgcgtt
    12541 ttttattctt tttattcacc cacatcgcaa acgtattcac tttatatgca ctttaaatgc
    12601 atatggtttg gttataactc cttgatttcc agttagcctc cgccgtttat gcacttttat
    12661 cactggctgg cacgaaccct gcaatctaca tttacagcgc aaacattact tattgttaac
    12721 atataaataa cgaattattt actgtagagg tcgctatgtc tcagccaatt acgcgtgaaa
    12781 actttgatga atggatgata cctgtttacg ctccggcacc ctttataccg gtacgtggcg
    12841 aaggttcgcg cttgtgggat cagcagggga aagagtatat cgacttcgcg ggtggcattg
    12901 cggtgaacgc gctgggccat gcgcatccgg aactgcgtga agcgctgaac gaacaggcga
    12961 gtaagttctg gcataccagc aacggttaca ccaacgagcc ggtactgcga ctggcgaaaa
    13021 aattgatcga cgccacgttt gccgatcgcg tcttcttttg taactccggt gcggaagcca
    13081 acgaagcggc gctaaaactg gcgcgtaaat tcgctcacga ccgctacggc agccataaga
    13141 gcggcatcgt ggcgttcaaa aatgcgtttc atggtcgcac gctgtttact gtcagtgcgg
    13201 gtgggcagcc agcctattca caggattttg cgccactgcc ggcggatatt cgtcatgctg
    13261 catataacga tattaactct gccagcgcgc tgattgacga ctctacctgt gcggtgattg
    13321 tcgaacccat ccagggggaa ggcggtgtgg tgccagccag caacgcgttt ttacaaggtc
    13381 tgcgtgaatt gtgtaaccgc cacaatgcgc tgttgatttt tgatgaagta caaaccggcg
    13441 tcgggcgcac cggggaactg tatgcctata tgcactacgg cgtgacgcct gatctgttaa
    13501 ctaccgccaa agcgctgggc ggcggtttcc cggtcggtgc gttgttggca accgaagagt
    13561 gcgcccgcgt gatgaccgtt ggcactcatg gcaccaccta tggcggtaat ccgctggcct
    13621 cggcggtggc aggcaaagtg ctggagctca tcaacacacc agagatgctt aatggcgtta
    13681 aacagcgtca cgactggttt gttgagcgtc ttaatactat taatcaccgc tatggtttgt
    13741 tcagtgaagt tcgcggctta ggtttgctga ttggctgtgt actgaatgcc gattacgccg
    13801 ggcaagcgaa acagatctct caggaagcgg cgaaagcagg cgtgatggta ctgattgcgg
    13861 gtggcaacgt ggtgcgtttt gcgcctgcgc tcaatgtcag cgaagaagag gtgacgaccg
    13921 gactggatcg ctttgcagct gcttgcgaac actttgttag ccgaggttca tcatgatggt
    13981 catccgtccc gttgagcgat cagatgtctc ggcgctgatg cagcttgcca gcaaaacggg
    14041 cggcggcctg acgtcgcttc ccgccaatga agccacgctt tcggcgcgta tcgaaagggc
    14101 aatcaaaacc tggcaaggcg aactgcccaa aagtgagcag ggctatgtgt tcgtgctgga
    14161 agatagcgag acaggcaccg tggcggggat ttgtgccatt gaggtggcgg ttgggctgaa
    14221 cgatccctgg tacaactatc gcgtcggcac gttggttcac gcctcaaaag agctgaatgt
    14281 ctataacgca ttgccgacgc tgtttctcag taacgatcac accggcagca gcgagctgtg
    14341 cacgctgttt ctcgacccgg actggcgcaa agagggcaac ggctatttgc tgtcgaaatc
    14401 gcgctttatg tttatggcgg cttttcgcga caagtttaat gacaaagtgg ttgctgaaat
    14461 gcgcggggtg attgacgaac acggctattc accgttctgg caaagcctcg gtaaacgctt
    14521 cttttcgatg gattttagcc gcgccgattt tctctgcggc accgggcaaa aggcatttat
    14581 tgcagaactg atgccgaaac atccgatcta tacccacttt ttatcccagg aagcccagga
    14641 cgtcatcggt caggtacatc cgcaaaccgc gcctgcccgc gcggtgctgg agaaagaagg
    14701 ttttcgttac cgcaactata tcgacatctt cgacggcggg ccaacgcttg agtgtgacat
    14761 cgaccgcgtg cgcgccattc gtaaaagtcg gctggtggaa gtggcagaag ggcagcctgc
    14821 gcagggcgat tttccggcct gcctggtcgc caatgaaaat tatcaccatt tccgcgtggt
    14881 gctggcgcgt accgatccgg caaccgagcg tttgatttta accgccgcac aactggatgc
    14941 cctcaaatgc cacgcagggg atcgcgttcg tctggtgcgc ctgtgcgcag aggagaaaac
    15001 agcatgactt tatggattaa cggtgactgg ataacgggcc agggcgcatc gcgtgtgaag
    15061 cgtaatccgg tatcgggcga ggtgttatgg caaggcaatg atgccgatgc cgctcaggtc
    15121 gagcaggctt gtcgggcagc ccgtgcggcg tttccgcgct gggcgcggct ctcattggct
    15181 gaacgtcagg tcgttgtcga acgctttgcc ggattgctgg aaaggaataa aggcgaatta
    15241 accgcaatca ttgcccgaga aaccggcaag ccgcgctggg aagcggcaac cgaagtgacg
    15301 gcgatgatca ataaaatcgc gatatcaatt aaggcgtatc acgctcgtac cggcgagcag
    15361 cgtagcgaaa tgccagacgg cgcggcgagc ctgcgacatc gcccgcacgg cgtgctggcg
    15421 gtgtttgggc cgtataattt ccctggtcat ttgccgaacg gacatatcgt tccggcattg
    15481 ctggcaggta acaccattat ctttaaaccc agcgaactga caccgtggag tggcgaagcg
    15541 gtaatgcgtt tatggcagca ggctggcttg ccgccgggcg tgctgaacct ggtgcagggc
    15601 gggcgtgaaa cgggtcaggc gctgagtgcg ctggaggatc tcgacggttt gctgtttacc
    15661 ggtagcgcca ataccggcta ccagctgcat cgccagctct ccggtcagcc ggagaaaatt
    15721 ctcgcccttg agatgggcgg taataatccg ctaattatcg atgaggtggc ggatatcgac
    15781 gcggctgtcc atctgaccat tcagtcggcg tttgtcacag ccgggcaacg ctgcacctgc
    15841 gcccgccgtt tattgctcaa aagcggagcg cagggcgatg cgtttcttgc tcgtctggtt
    15901 gccgtcagcc agcgattaac gccgggcaac tgggatgacg aaccgcagcc gtttattggc
    15961 gggctgattt ctgaacaggc cgcacagcag gtggttactg cctggcagca actggaagcg
    16021 atgggcggac gaaccctgct tgcgccgcgc ttattacaat cagagacatc gttgctgacg
    16081 ccggggatca ttgaaatgac aggcgttgct ggcgtaccag atgaagaggt gttcggaccg
    16141 ttattgcgcg tctggcgtta tgattctttc gaggaagcga ttctaatggc gaataacact
    16201 cgcttcggac tctcttgcgg tctggtttcc cccgagcggg aaaaattcga tcaactgttg
    16261 ctggaggcgc gggcggggat tgttaactgg aacaaaccgc ttactggtgc tgccagtacc
    16321 gcgccattcg gcggcattgg tgcatccggt aaccatcgcc ccagcgcctg gtatgccgca
    16381 gattactgcg catggcctat ggcgagcctg gagtcggact cgttaacttt gcccgcaacg
    16441 cttaaccccg ggctggattt ttccgatgag gtggtgcgat gaacgcctgg gaagttaatt
    16501 tcgacgggct ggtagggctg acgcatcatt acgctggcct gtcgtttggt aatgaagcct
    16561 ctacccgtca ccgttttcag gtgtctaacc cgcgacaggc ggcaaaacag ggcttactga
    16621 aaatgaaagc ccttgccgat gcgggattcc cccaggccgt gatcccgccg cacgagcgtc
    16681 cgtttattcc ggtgctgcgt cagttgggat tcagtggtag cgatgagcag gtactggaaa
    16741 aagttgcacg ccaggcaccg cactggcttt ccagcgtcag ttccgcttcg ccaatgtggg
    16801 tagccaatgc ggcaacgatc gcgccatctg ccgatacgct ggatggcaaa gtgcatctca
    16861 ccgttgccaa cctgaacaat aaatttcacc gctcgctgga agcgcacgtc accgaatcac
    16921 ttttaaaagc gatttttaac gacgaagaga aatttagcgt tcattcggcg ttgccgcagg
    16981 tagcgttgct cggtgatgag ggggcggcaa accacaatcg tctcggcggt cattacggtg
    17041 agccgggtat gcaacttttt gtctacgggc gagaagaagg caatgatacc cggccttccc
    17101 gttatccggc gcgacagact cgcgaagcca gcgaggcggt ggcaaggctg aatcaggtga
    17161 atccccaaca ggtgattttc gcccagcaaa acccggacgt tatcgaccag ggcgtttttc
    17221 ataatgacgt gattgccgtg agtaaccgcc aggtgctgtt ttgccaccaa caggcgttcg
    17281 ctcgccagtc acagttactg gcaaacctgc gtgcgcgggt gaacggtttt atggcgatag
    17341 aagttccggc aactcaggtt tccgtgtcgg atgcggtgtc tacctatctg tttaacagcc
    17401 aactgttgag ccgcgatgat ggttccatga tgttggtgct gcctcaggag tgtcgggaac
    17461 acgccggagt atggggttat ctcaatgaac tccttgccgc tgacaacccg attagcgaac
    17521 taaaagtctt tgatttacgt gaaagcatgg cgaatggcgg cggcccggcg tgcctgcggt
    17581 tgcgggtggt attgacagaa gaagaacgcc gggcggtgaa tccggcggtg atgatgaacg
    17641 atacgctgtt taatgcgctc aatgactggg tggatcgtta ctaccgcgat cgccttactg
    17701 ctgccgatct ggccgacccg caattgctgc gcgaagggcg ggaagcactg gatgtattga
    17761 gccaattact gaatctcggt tcggtttatc cgttccagcg cgagggaggg ggcaatggat
    17821 aattttcttg ctctgacctt aacgggtaaa aaaccggtta tcaccgagcg agaaatcaac
    17881 ggcgttcgct ggcgctggct gggcgatggt gtgctggaac tgacgccatt aacgccaccg
    17941 caaggcgcac tggtgatttc agcgggaata cacggtaatg agacggcacc tgtggagatg
    18001 ctggacgcgt tgcttggcgc gatatctcac ggcgagatcc cgttacgttg gcggttgctg
    18061 gtgatcctcg ggaatcctcc tgcgctgaag caagggaaac gttattgcca tagcgatatg
    18121 aatcgaatgt ttggcggtcg ttggcagcta tttgctgaaa gcggagaaac ctgtcgggcg
    18181 cgcgaactgg aacagtgcct ggaagatttt tatgacctgg gcaaagaatc tgtgcgctgg
    18241 caccttgatc tacataccgc aattcgtggc tccttgcatc cgcagttcgg tgtattaccg
    18301 caacgcgaca ttccctggga cgagaaattt ctgacgtggc tgggtgcggc ggggctggag
    18361 gcgctggtgt tccatcagga acctggtggt acgtttaccc atttcagcgc cagacatttt
    18421 ggcgcgctgg cctgtacgct ggaacttggc aaagcgttgc cctttgggca aaacgatctt
    18481 cgccagtttg cagtaactgc cagcgcaatt gctgcgctgc tatctggtga gagtgtcggt
    18541 atcgtgagaa caccgccgct ccgttatcgg gtggtttcgc aaattactcg ccactcgccg
    18601 tccttcgaaa tgcatatggc aagtgacacg ctgaatttta tgccgtttga gaaaggaaca
    18661 ttgctggcgc aggacggaga ggaacgtttt accgtaaccc atgatgtaga gtatgtgtta
    18721 ttccctaatc cgttggtagc gttgggatta cgcgcgggat taatgctcga aaaaataagc
    18781 taatacttac ccgcagaaat cattctgcgg gtattaagtt gc
//
LOCUS       DAPHJE010000087        18746 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPHJE010000087 DAPHJE010000000
VERSION     DAPHJE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.087"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..1125
                     /gene="phoE"
                     /locus_tag="RH222_003123"
     CDS             70..1125
                     /gene="phoE"
                     /locus_tag="RH222_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV3798109.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1164..1565)
                     /gene="crl"
                     /locus_tag="RH222_003124"
     CDS             complement(1164..1565)
                     /gene="crl"
                     /locus_tag="RH222_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV3798110.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="RH222_003125"
     CDS             complement(1623..2867)
                     /gene="frsA"
                     /locus_tag="RH222_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV3798111.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="RH222_003126"
     CDS             complement(2959..3417)
                     /gene="gpt"
                     /locus_tag="RH222_003126"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV3798112.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3678..5135
                     /gene="pepD"
                     /locus_tag="RH222_003127"
     CDS             3678..5135
                     /gene="pepD"
                     /locus_tag="RH222_003127"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV3798113.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(5192..5544)
                     /gene="prfH"
                     /locus_tag="RH222_003128"
                     /pseudo
     CDS             complement(5192..5544)
                     /gene="prfH"
                     /locus_tag="RH222_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5540..5746)
                     /locus_tag="RH222_003129"
                     /pseudo
     CDS             complement(5540..5746)
                     /locus_tag="RH222_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6065..6517)
                     /locus_tag="RH222_003130"
     CDS             complement(6065..6517)
                     /locus_tag="RH222_003130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3798114.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
     gene            complement(6514..7569)
                     /gene="dinB"
                     /locus_tag="RH222_003131"
     CDS             complement(6514..7569)
                     /gene="dinB"
                     /locus_tag="RH222_003131"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV3798115.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7640..8425)
                     /gene="lafU"
                     /locus_tag="RH222_003132"
     CDS             complement(7640..8425)
                     /gene="lafU"
                     /locus_tag="RH222_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV3798116.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            8397..10109
                     /locus_tag="RH222_003133"
                     /pseudo
     CDS             8397..10109
                     /locus_tag="RH222_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(10427..10924)
                     /gene="rayT"
                     /locus_tag="RH222_003134"
     CDS             complement(10427..10924)
                     /gene="rayT"
                     /locus_tag="RH222_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDV3798117.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(11100..11849)
                     /locus_tag="RH222_003135"
     CDS             complement(11100..11849)
                     /locus_tag="RH222_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV3798118.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGGDLGDGQFIESPRT
                     GENIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            12150..12890
                     /locus_tag="RH222_003136"
     CDS             12150..12890
                     /locus_tag="RH222_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV3798119.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(12861..13628)
                     /locus_tag="RH222_003137"
     CDS             complement(12861..13628)
                     /locus_tag="RH222_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV3798120.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13834..14412)
                     /gene="lpcA"
                     /locus_tag="RH222_003138"
     CDS             complement(13834..14412)
                     /gene="lpcA"
                     /locus_tag="RH222_003138"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV3798121.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            14652..17096
                     /gene="fadE"
                     /locus_tag="RH222_003139"
     CDS             14652..17096
                     /gene="fadE"
                     /locus_tag="RH222_003139"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV3798122.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLCSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(17139..17585)
                     /gene="ivy"
                     /locus_tag="RH222_003140"
     CDS             complement(17139..17585)
                     /gene="ivy"
                     /locus_tag="RH222_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV3798123.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            17766..18536
                     /gene="yafV"
                     /locus_tag="RH222_003141"
     CDS             17766..18536
                     /gene="yafV"
                     /locus_tag="RH222_003141"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV3798124.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQNDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(18578..>18746)
                     /locus_tag="RH222_003142"
     CDS             complement(18578..>18746)
                     /locus_tag="RH222_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3798125.1"
                     /translation="IRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNY
                     LASVLAESGLS"
BASE COUNT         4504 a         4820 c         4907 g         4515 t
ORIGIN      
        1 aaaactttcc tgttttcaac ggggctctcc cgctgaatat tcgcgcgtta attaaaatca
       61 ggaatgaaaa tgaaaaagag cactctggca ttagtggtga tgggcattgt ggcatctgca
      121 tccgtacagg ccgcagaaat atataacaaa gacggtaata aactggatgt ctatggcaaa
      181 gttaaagcca tgcattatat gagtgataac gacagtaaag atggcgacca gagttatatc
      241 cgttttggtt ttaaaggcga aacacaaatt aacgatcaac tgactggcta tggccgttgg
      301 gaagcggagt ttgccggaaa taaagcggag agtgatactg cacagcaaaa aacgcgtctc
      361 gcttttgccg gattgaagta taaagatttg ggttctttcg actatggccg taacctgggc
      421 gcgttgtatg acgtggaagc ctggaccgat atgttcccgg aatttggtgg cgactcctcg
      481 gcgcagaccg acaactttat gaccaaacgc gccagcggtc tggcgacgta tcggaacacc
      541 gacttcttcg gcgttatcga tggcctgaac ttaaccctgc aatatcaagg gaaaaacgaa
      601 aaccgcgacg ttaaaaagca aaacggcgat ggcttcggca cgtcattgac atatgacttt
      661 ggcggcagcg atttcgccat tagtggtgcc tataccaact cagatcgcac caacgagcag
      721 aacctgcaaa gccgtggcac tggcaagcgt gcagaagcat gggcaacagg tctgaaatac
      781 gatgccaata atatttatct ggcaactttt tattctgaaa cacgcaaaat gacgccaata
      841 actggcggct ttgccaataa gacacagaac tttgaagcgg tcgctcaata ccagtttgac
      901 tttggtctgc gtccatcgct gggttatgtc ttatcgaaag ggaaagatat tgaaggtatc
      961 ggtgatgaag atctggtcaa ttatatcgat gtcggggcta catattattt caacaaaaat
     1021 atgtcagcgt ttgttgatta taaaatcaac caactggata gcgataacaa attgaatatt
     1081 aataatgatg atattgtcgc ggttggcatg acctatcagt tttaatgatt attgccggat
     1141 gtgatgcatc cggcacattt cactcacgcc gttaacttca ccggctcgtc acgaaaatca
     1201 tccgccggtt ccagcttcag attcagcgtc gtcagcagtt cacgcagctt ctcgtgaaac
     1261 tcacgcaggg tgtgctccag tcgttcaacc acttcagtgt cttttaccgg aacgctcttc
     1321 cagtcgcctg ctttatcgaa cagaccaaac tggtagctgt aggtaaaacg ggattcctgc
     1381 gcttccagct ccatccacca gccccagaat tcacgcactt ccggtgccgg tttcacgttg
     1441 acgcatacag ccagacaatc gaaaaagaat cgattatctt cgcacttacc ttcacgaata
     1501 tacgggccta gtgcggtaaa ttttttgatc aatctgctct tcgggtgtcc actcggtaac
     1561 gtcattgcta tctcctgttg tgatgcaact gttttaccaa attggcaaaa tttagcaaat
     1621 ttttaacaca agcgtttttc gatccagccg gtgatttcct gaagaccttt gtcaaaattc
     1681 cgatacaccg ggttaaatgg gatctctaat aatttaccgt cagcagatga tgaggtgatt
     1741 aagcgtgagt cctcttccgg gctgaacgga tcgttcttcc agtagcctga taacattggc
     1801 gttgggcagc gacgtcccag caatccttgc acttttaatg aatagcgatt cagctccacg
     1861 cgcaacgcgt catcggaagc atcatgcatc cccaaacgac tcgccagaac gtcaagatac
     1921 atttccggca cctgttgctg gcacttaaaa tcactcaaca gggtatgaac taccggacca
     1981 agacaggcaa ccgctttcag acgcggcgat tcaaggtatg ccagacgcac ggcaacgtta
     2041 gcgccgaaac ggaaaccaaa ggccgcgacg cgagtgtgat ccacccacgg tacgttaggc
     2101 agcgccttta agacgtgctg atgcaacagg ctggagtcct gggtgagctt ccattttgaa
     2161 gaaaagccca ccgacggcat atcaatagtc agcatcgcaa tgccgcgcgg cgcaaaataa
     2221 cgttcataca ggctgtaata gtccgtctgc atcgcatcca gaccaccaca cattaatact
     2281 gtcgggaacg ggccatcgcc tttcggcata tgcaaaaagc cggtgatggg cgcaccgccg
     2341 ggtacggtaa actccatctg ccgcatcgtg cccggcagac gctgggcggc ctcttcatag
     2401 gcgcggtttg ataaagcctg cgcttgctcg gccaggtcat ctcctttcag atgaggatag
     2461 gcggcaatgt tgtacaacgt agccgcatgc agccagtgtc gaccactgag ttgcggatct
     2521 tcttccgcac aggctttttg ttgccacacc atcgcctggg tggcccactc ataaatccag
     2581 ttgccaccac ggtagccaat cacggtatcg tataaatcat catcggtacg ttcagcatcg
     2641 ctcatcacaa tacgtgcctg gacgtcgagg atttcacgtg ggtcaatgcc gcgccagatc
     2701 cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc aagggccgac
     2761 tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt ttcaggatgt
     2821 ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga tgcagcctcc
     2881 agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa cgcccggcag
     2941 tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga cgcccatatc
     3001 ccacggctgt tcgatccagg tatcttgcgg gatatcaaca acataattat caaccagcgg
     3061 acgaccagcc ggttttgcaa agatggtgac aaagtgcgct tttggataca tttcacgaat
     3121 cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc cttcgccatc
     3181 gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt agctggaaat
     3241 acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac ccggtaccag
     3301 accgccacgg cttacggcaa taatgccttt ccattgttca gaaggcatca gtcggcttgc
     3361 gagtttacgt gcatggatct gcaacatgtc ccaggtgacg atgtattttt cgctcatgtg
     3421 aagtgtccca gcctgtttat ctacggctta aaaagtgttc gaggggaaaa taggttgcgc
     3481 gagattatag agatctggcg cactaaaaac cagtatttca catgagtccg cgtcttttta
     3541 cgcactgcct ctccctgacg cgggataaag tggtattctc aaacatatct cgcaagcctg
     3601 tcttgtgttg acaacatttt ctgctaaccc tgtgacctgc aatactgttt tgcgggtgat
     3661 cgacaaggag acttaacgtg tctgaactgt ctcaattatc tccacagccg ctgtgggata
     3721 tttttgccaa aatctgttct attcctcacc cgtcctatca tgaagagcaa ctcgctgaat
     3781 acattgttgg ttgggcaaaa gagaaaggtt tccatgtcga acgcgatcag gtaggtaata
     3841 tcctgattcg taaacctgct accgcaggta tggaaaatcg taaaccggtc gtcttgcagg
     3901 cccacctcga tatggtgccg cagaaaaata acgacaccgt gcatgacttc acgaaagatc
     3961 ctatccagcc ttatattgat ggcgaatggg ttaaagcgcg cggcaccacg ctgggtgcag
     4021 ataacggcat tggtatggcg tctgcgctgg cggttctggc tgacgaaaac gtggttcacg
     4081 gcccgctgga agtgctgctg accatgaccg aagaagccgg tatggacggt gcgttcggct
     4141 tacagagcaa ctggttgcag gctgatattc tgattaacac cgactccgaa gaagaaggtg
     4201 aaatctacat gggttgcgcg gggggtatcg acttcacctc caacctgcat ttggatcgtg
     4261 aagcggttcc agctggcttt gaaaccttca agttaacctt aaaaggtcta aaaggcggtc
     4321 actccggcgg tgaaatccac gttggcctgg gtaatgccaa caaactgctg gtgcgcttcc
     4381 tggcgggtca tgcggaagag ctggacctgc gccttatcga tttcaacggt ggcacactgc
     4441 gtaacgccat cccgcgtgaa gcctttgcga ccattgctgt cgcagctgat aaagtcgacg
     4501 ccctgaaatc tctggtgaat acctatcagg acatcctgaa aaacgagctg gcagagaaag
     4561 agaagaatct ggccttgttg ctggactctg tagcgaacga taaagctgct ctgattgcga
     4621 aatctcgcga tacctttatt cgtctgctga acgccacccc gaacggtgtg atccgcaatt
     4681 ccgacgtggc aaaaggtgtg gtcgaaacct ccctgaacgt cggtgtggtg accatgactg
     4741 acaataacgt agaaattcac tgcctgatcc gttcactgat cgacagcggt aaagactacg
     4801 tggtgagcat gctggattcg ctgggtaaac tggctggcgc gaaaaccgaa gcgaaaggcg
     4861 catatcctgg ctggcagccg gacgctaatt ctccggtgat gcatctggta cgtgaaacct
     4921 atcagcgcct gttcaacaag acgccgaaca tccagattat ccacgcgggc ctggaatgtg
     4981 gtctgtttaa aaaaccgtat ccggaaatgg acatggtttc tatcgggcca actatcaccg
     5041 gtccacactc tccggatgag caagttcaca tcaaaagcgt aggtcattac tggacactgc
     5101 tgactgaact gctgaaagaa attccggcga agtaattatt tgatttgctg ccggatggcg
     5161 tttaatcgcc ttccggcagt ttcatccttc attatccttc gataaaagcc atccctgtaa
     5221 atgtccgtcg cgggttgcca cgttcaatct ggtgatggaa cattcgccgc tgcgatttca
     5281 gcgccgcgct attttcctgt tgctgctgct ccagcttcca ggcaatcagc aatcgcgcca
     5341 gccgtttgtt agcatgccca gaaaccagtt tttgcgccca tgatgagacc gatacggact
     5401 cggacaaatc cactgaatag tgccgcacca gctttcgctt aatgcccatg cgttatcgcc
     5461 atccagagaa accagcgccg aacgcagcgt gtcagagtag cggcccgttt ctgtttccag
     5521 caccgttacc gcgacgtctt gccggataaa aacgaccaac agagaagaac gctgcgccaa
     5581 tcggatagcc gcgtccggga tgtaaatctg gcatccccac tacgcgctgc atgttgggta
     5641 aatttgccgt ggtgtgtaat tgttggatcg ctaaactctc gatccacagg tcatcagatg
     5701 cgatggtaaa taccgcatcg gataagggac gaatatattt gcccatgtac cattccattt
     5761 atactcgtca tacttcaagt tgcatgtact gcgactgcgt tcgttcaccc cagtcactta
     5821 ctttagtaag ctcctggcga ttcactcgct tgtcaccttc ctgcaactcg aattatttag
     5881 agtatataaa tattaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat
     5941 taatcctgga aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt
     6001 gcagtttgtg gaataagaaa acgcgcacat accaggcggg cgttattttc attgcaagct
     6061 ggatttaatg ttgcggttta tatcgcatat aaaaattagt aaaccacgct ccccggcatt
     6121 caacgtgctg ctgcttaact atctgaaaac cataacgttc aaaaaagggt tttgcggtta
     6181 tgctggcgtc caccgtaagt tcggattcag acttaatcaa aggttttaac aaggcgctgg
     6241 caaccccgcg gcgggtgtat tcagggtcaa caaataacat atcgatatga cgttcaatgc
     6301 gggaaataaa accaaccggt tgtgcattaa tgactgcaac ccgcacttgt gatttcgcga
     6361 gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc ggcaatttgt tgtggtgaat
     6421 aatgctgact ggcggtcatc ataaccgctc taatgaaaat agcgcatagt tgctgaaaat
     6481 cgccaggctg atagtttctt atttgtatgt tattcataat cccagcacca gttgtctttc
     6541 catttgaggg tcaagcaacg tcacatgcag ccccaccagt cgcacaccgc gcccgccgcg
     6601 gcgttcatcc caggttttac gcgcggtggc gattagatca gctttattca gccgcggcca
     6661 gacgtgctcc tgggtggttt gctgaaaatc gtcgaacttt aatttcaccc cctggcgagc
     6721 aatcagtaaa tcaggtttta cctttgccag acggcgttca agttccggat acagccgctc
     6781 gataatcgct tcacattcag accagtgatg aatatcttcc gccatcgtgc gttccacacc
     6841 gacggatttt cgcaaccgtt cgctgttaac gtcgcgttcg tcaatcccct gactacgctc
     6901 ccacaaaatg cggccaaatt tgccaaagcg tttaagcaga atcaccagat cacacttttg
     6961 cacatcaccg caggtccgca gccccatcgc ttccagtttt gccgccgaga ctttgccgac
     7021 gccggggatt tttgccaatg gtaaggtttg cagaaatgcc ggaacttctg ccggagtaat
     7081 cacaaactgg ccgttgggtt tattcatgtc ggaggcgatt ttggcgagaa acttgaccgg
     7141 cgcaacgccc gcagacgccg tcaggtgcag ctcgttgaaa atcgtctggc ggatttcctg
     7201 ggcgatgagg gtcgcagaac cgtggcaatg gacgctatcg gtgacatcga gataagcctc
     7261 atccagtgac aacggttcaa tgcgcgaggt gtagcgcgag aagatttcac ggatatgatt
     7321 tgaggcttct ttgtaggcgt caaagcgccc cggaagcaag gtgagatgtg ggcataattt
     7381 gagcgccatc ccagtaggca tagcgctacg tacgccaaat ttacgcgcgg gataattggc
     7441 ggtgctgatc accccccgac gttcgcggct gccgccaata gcaataggga tatcgcgcag
     7501 ggcgggattg tcgcgcatct ccactgcggc gaaaaagcag tccatatcca catgaatgat
     7561 tttacgcatt gctcacctct caacactggt aaagtataca gtgatttcag ggtttgagaa
     7621 atgcgtaaag attcagcatt tagcgcattc gctgcgaaag cacctgctgc ttatccagct
     7681 tttgcaccag cggctgcacc actttatccc catgctgacc aaagtattga tacagcgtat
     7741 cggacgcact tttggtcagc accataatct caatacgccg attgcccgcg ctttgcggat
     7801 ttttggcatc cagcagcatc tggtccgcca ttgcgcttac ctgcatcact ttatcttccg
     7861 gcattccggc ctcttccagc acccgacgag ctgaaagcgc gcgatcgccc gaaaggttcc
     7921 agttgttgta gatattgttt ttgtaggcca tcgcatcggt atgcccggta ataataattt
     7981 tgttatcgag cgagtcgaac actggcgcga gctccaccag caaagtttta aagaacggca
     8041 taatctgcgc gctgccgcgt tcgaacatat tgcggttctg gtcatcttta atcagcacgc
     8101 gcaatccctg gggaacaatc tccatctcca gattcgcttc catatgcgca tcacgggcaa
     8161 tggtgttgat actggttgcc agctcgccaa gctcggtggc ggatttcttc ttcagcagcg
     8221 ctgttttctc gttgacgtct cgcgcttttt tctccgtctc ttccggcacc gccacggtgg
     8281 caggttttgg cggatgagac gggctgattt tatttaacgg cgacaatccg ccgccactga
     8341 aaatcgactg cccgtgcagc gcggcgataa tgctttcacg ctcggattta ctgacgctgt
     8401 tgacaatcaa ctttatcgtc gtcaccaaag gggccgagcg tatttccgag gtttctgccc
     8461 gctttaccct ggacgcgatg cccggcaaac agatggcgat tgacgccgac cttaacgccg
     8521 ggttgatcaa ccaggcgcag gcgcaaaccc ggcgtaaaga tgtcgccagc gaggccgatt
     8581 tctacggcgc gatggacggg gcgtcaaagt ttgtgcgcgg ggatgccatc gccgggatga
     8641 tgattctggc aatcaacctg atcggcggcg tctgtatcgg gatcttcaaa tacaacctga
     8701 gcgccgatgc tgccttccag cagtatgtgc tgatgaccat cggcgacggg ctggtggcgc
     8761 agatcccttc cctgctgctc tccaccgcgg cggcgattat tgtcacccgc atcagcgaca
     8821 acggcgatat cacccatgac gtgcgccacc agctgctggc aagcccgtcg gtgctctaca
     8881 ccgctaccgg gatcatgttc gtgctggcag tggtgccggg gatgccgcac ctgccgtttt
     8941 tgctgttcag cgccctgctt ggctttaccg gctggcggat gagcaaacgc ccgcaggcgg
     9001 cggaggcgga agaaaaaagc ctcgaaacgc tgacccgcac catcaccgaa accagcgagc
     9061 aacaggtcag ttgggagacg ataccgctga tcgagcctat cagcttaagc ctcggctaca
     9121 agctggtggc gctggtggat aaagcgcagg gcaacccgct cacccagcgg attcgcggcg
     9181 tgcggcaggt gatatccgac ggtaacggcg tgctgctgcc ggagatccgt attcgggaaa
     9241 acttccgcct caagcccagc cagtacgcta ttttcatcaa cggcattaag gctgatgaag
     9301 cggatattcc ggcggataaa ctgatggccc taccctccag cgaaacctac ggcgaaattg
     9361 acggcgtgct ggggaacgac ccggcgtacg ggatgccggt gacctggatc cagcccgcgc
     9421 agaaagcgaa ggcgctgaat atggggtatc aggtgatcga cagcgccagc gtgattgcca
     9481 cgcatgtgaa taagattgtg cgtagctata ttcctgattt atttaactat gacgatatta
     9541 cgcagttgca taaccgtttg tcgtcgatgg caccgcgcct ggcggaagat ttaagcgcgg
     9601 cgctgaatta cagccagttg ctgaaagtgt accgcgcgct gctgaccgaa ggcgtttccc
     9661 tgcgcgatat cgtcaccatt gccaccgtgc tggtcgccag tagcgcggtg accaaagatc
     9721 atattctgct ggcagctgat gtgcgcctgg cgctgcggcg aagcattacc catccgttcg
     9781 ttcgcaagca ggagctgacg gtgtatacgc tgaataatga gctggaaaat ctgctgacca
     9841 acgtggtgaa tcaggcgcaa cagggcggga aagtgatgct cgacagcgtg ccggtcgatc
     9901 cgaatatgct caaccagttc cagagtacga tgccgcaggt aaaagagcag atgaaagcgg
     9961 cggggaaaga tccggtgctg ctggtgccgc ctcagctgcg ccctttgctg gcgcgttatg
    10021 caaggttgtt tgcgccgggg ctgcatgtgc tgtcgtataa cgaagtgccg gacgagctgg
    10081 agttgaagat aatgggggcg ttgatgtaag tatccggcat tggtgcacaa ctgccggatg
    10141 cggcgtgaac gccttatccg tcctactgtt caggcacgat tcgtaggccg gataagcgca
    10201 agcgcatccg gcaccagcgc acaaccgccg gatgcggcgt aagcgcctta tccggcctac
    10261 cgatccagca caggtttgta ggcatgataa gacgcgtcag cgtcgcatca ggcatctgcg
    10321 cataaccgcc ggatgcggcg taaacgcctt atccggccta ccgatccggc acaggtttgt
    10381 aggcatgata agacgcgcca gcgtcgcatc aggcatctgc gcataattat aaaatgatac
    10441 gctcgccagc atttatatcc gttacgtccc ccgcccaatc gatgggatat aaccctcgcg
    10501 cgacatcgcg atggaacgtt gagaatggcc aatcactcac ttgctttacc caaccatgct
    10561 ttactggatt tatataaata taatcaacat gatgcctata atctttggta ttgcggatgg
    10621 cgtgctccca aaaacgtggt tgccagatat ttttcaatcc acaagcatgg gtaaattgct
    10681 ttttaatttc ccgccagcgc gaggaaaaat catcatcgcc ttcaggtaat gtccagatac
    10741 agtgcatatg ctctggcaaa acgacccagg cgttgatttc aaaaggcctg tctcgcttaa
    10801 ctttaataat ggcgtgacgg agcatctggt actgggtggt caagagttgg cttcgacgat
    10861 tgcgtaaatt caccgtgaaa aaccaggttc cccccttgat gtaataacga cgatattcag
    10921 acatggcgac tcctttttct caacggtaag aagaaaagga aaggtcggta atgaggtgat
    10981 ggaataacag gaaagcggct cgcaaaaacg cacagatttg taggcatgat aagacgcgtc
    11041 agcgtcgcat caggcatccg tgcacaaccg ccggatgcgg cgtaaacgcc ttatccggcc
    11101 tacaaaatcg tctcttccgt caagatccgc cgcgcgccca aaaaatgatc ctgccagaaa
    11161 ggttcggcta accggcttac ccgaatgttt tccccggtgc gcggcgactc gataaactgc
    11221 ccatcgccca gatccccccc catatgatcg gcaatttcac ggctgtggat atggaaaaac
    11281 agcaaatctc cccggcgcag gtcgttattc gccacaatcg ttgcccgatg atagtgatac
    11341 atctcattgg ccgtgcgcgg gagcttcgcc tcaaggatct tgttgtaggc ataaaaaacc
    11401 aacccgctac agtcgaagcc ttgatcgggc cgcgtgccgc cccagacgta aggcttgcca
    11461 agctgttgct ccagacggtg aatcgccact tcggtgatgt tatgcagatg gtcgctgctt
    11521 aaaaagtgat tgttctccgc cagcgcctgc cagcgtcggt cgctcgcctt gagcggcgca
    11581 ggaaaccatt ctggatgctg ttttatctgc tcccggttgt gctggcgtag cgcccttctg
    11641 ctttcggcat tgccttccac aatatagctg gcctgttttt tcagatgggt ttggtactgt
    11701 tttaataaac gcgcacggtt ttgcatccgc tggcgggcgg cgtagctgaa agagatatgt
    11761 gatgtggtgg cgctggcgaa taaagggagc aaaacgcata acgaaaacac gcctggatgc
    11821 agaaatctgc cgagcaagaa cgaagacatg aaagacataa accggctgaa tgaaggtgag
    11881 aattttaaaa gaccggagga gtaaacggga ttattaattg cttatttgca tattactatt
    11941 gaggtttcat aatcttagga aataaatgat acatgcgctt ttcggactat tgaaaactgc
    12001 ccctgtgcta aatttgtaaa ccctgcgccg acaggcttgc gtaaaactct tttggtgata
    12061 atgtgagcct tccaaatcca gactattccg atttgggatc cattttgccg tcctggcgtg
    12121 tgtaaccgtt ttatcaagga ataagcagta tgcgtaaaat cgcattaatt cttgcgatgt
    12181 tgttgatccc gtgcgtttcg tttgccggtt tgctgggcag cagtagctcg accacgccag
    12241 tgagcaaaga gtataagcag cagttgatgg gatcccctgt ctacatccag atcttcaagg
    12301 aagaacgtac gctcgatctc tacgtcaaaa tgggcgagca atatcagctg ctcgacagtt
    12361 ataaaatctg taaatattcc ggcggcttag ggccaaaaca gcgtcagggc gatttcaaaa
    12421 gcccggaagg gttttatagc gtccagcgta atcagttaaa accagacagc cgttactaca
    12481 aagcgattaa tattggtttc cccaatgcct atgaccgtgc gcatggttat gaagggaaat
    12541 acctgatgat ccacggcgat tgtgtttcca tcggctgcta cgcgatgacc aatcagggca
    12601 ttgacgagat tttccagttc gttactggcg cactggtatt tggtcagccg agcgtgcaag
    12661 tgagcattta cccgttccgc atgaccgacg ctaatatgaa gcgccacaaa tattccaact
    12721 ttaaggactt ctgggagcaa ctgaagccgg gttacgacta ctttgagcaa acccgtaagc
    12781 caccgaccgt ttctgtggtc aatggtcgtt acgtggtcag caagccgtta agccacgaag
    12841 ttgtgcaacc acagctggca tcaaactaca cgctccccga ggcaaaataa gcgccattcg
    12901 cctggcataa ttttttgcca ggtttcattg cccgtcagcg gctgtgtcgc aatcaccgtg
    12961 accacatcat tcggtgtggt ctgcgagctg aagtcgattt ccacatcctg atccagcaac
    13021 gttgccacgc caaacggcgc gcggcgggtg atccagtgta aattagtcga gcaatacgcc
    13081 attacatagc gcccgtcaga aagcagcatg ttgaaaacgc ccttctgccg cagttcatcc
    13141 gccagtgagg cgatatattt aaataccgcc gccatgttgc ccggtgtgcg cgggtaacgc
    13201 tgcgttaatt tatgcaggag ccagcaaaag gctttttcgc tgtcggtttc gcctaccggg
    13261 cggaagttgc cggtttccag tgatttgtag cccgtcagtt gtccgttatg ggcataagtc
    13321 cagttacgcc cccataactc gcgggtaaat gggtgagtat tttccagcgc cacctcgccc
    13381 cgattagcct ggcgaatatg agccaccacc gaacaggatt ttatcgggta gtcctggaca
    13441 agtttggcga tgggggaatt aaagctgggt tgtggatctt taaatgtgcg acagccttta
    13501 ccttcgtaaa aggtaatgcc ccagccatct ttatgtggcc cggttccacc accacgctgt
    13561 acaagcccgg tgaaactaaa gcagatatcg gtagggacgt tggcgctcat cccgagcaat
    13621 tcgcacatgg gtaacctcca caacagcggg agcgcatggc ccccggcgaa gattgattta
    13681 agaattttca ggtcggatgc ggcgtgaacg cattattcgg cctacggttc gcgtacagat
    13741 ttgtaggcat gataagacgc gtcagcgtcg catcaggcat cggtgcacaa ttgccggatg
    13801 cggcgtaaac gtcttatccg gcctacgcca gacttactta accatctctt tttcaatcaa
    13861 ctggatcagg atatggatca ctttaatgtg aatctcctga atgcggtcgg cataaccaaa
    13921 gtgcggtacg cgaatttcga tatccgccgt gccagccatt ttgccgccgt ctttaccggt
    13981 cagggtgatc actttcattc ccttctcacg cgccgctgcg atcgctttga tcacgtttgc
    14041 agagttaccg gaggtggaga tccccagcag tacatcgcct tcgcgaccta ccgcttcaac
    14101 gtaacgggag aaaatatcat tgaaaccgaa atcgttaccg acgcatgaaa tatgactaac
    14161 gtcagaaata gcaatcgccg ggtagcccgg acggttttca cggtagcgac cggtcaactc
    14221 ttcggcaaag tgcatagcgt cgcaatggga accgccgttg ccgcaggaaa gcactttgcc
    14281 accggcttta aagctgtctg ctaacaggac cgccgcgcgc tgaatggcgt gaatattggc
    14341 gtcatctttt aaaaagttag ccagcgtttc cgccgcttcg ttcagttcgt tacgaataag
    14401 atcctggtac atgaggatat ccttcagcat aaatgtaata gacaaaatgc agtgtaccgg
    14461 ataccgccaa aagcgagaag tacgggcagg tgctatgacc aggacttttt gacctgaagt
    14521 gcggataaaa acagcaacaa tgtgagcttt gttgtaatta tattgtaaac acattgctaa
    14581 atgtttttac atccactaca accatatcat cacaagtggt cagacctcct acaagtaagg
    14641 ggcttttcgt tatgatgatt ttgagtattc tcgctacggt tgtcctgctc ggcgcgttgt
    14701 tctatcaccg cgtgagctta tttatcagca gtctgatttt gctcgcctgg acagccgccc
    14761 tcggcgttgc tggtctgtgg tcggcgtggg tactggtgcc tctggccatt atcctcgtgc
    14821 catttaactt tgcgcctatg cgtaagtcga tgatttccgc gccggtattt cgcggtttcc
    14881 gtaaggtgat gccgccgatg tcgcgcactg agaaagaagc gattgatgcg ggcaccacct
    14941 ggtgggaggg cgacttgttc cagggcaagc cggactggaa aaagctgcat aactatccgc
    15001 agccgcgcct gaccgcagaa gagcaagcgt ttctcgacgg tccggtagaa gaagcctgcc
    15061 ggatggcgaa tgatttccag atcacccatg agctggctga tttgccgccg gagttgtggg
    15121 cgtaccttaa agagcatcgt ttcttcgcga tgatcatcaa aaaagagtac ggcgggctgg
    15181 agttctcggc ttatgcccag tctcgcgtgc tgcaaaaact ctccggcgtg agcgggatcc
    15241 tggcgattac cgtcggcgtg ccgaactcat taggcccggg cgagctgctg caacattacg
    15301 gcactgacga gcagaaaaat cactatctgc cgcgtctggc gcgtggtcag gagatccctt
    15361 gctttgcact gaccagcccg gaagcgggtt ccgatgcagg cgctatcccg gataccggga
    15421 ttgtctgcat gggcgaatgg cagggccagc aggtgctggg gatgcgtctg acctggaaca
    15481 aacgctacat tacgctggca ccgattgcga ccgtgcttgg gctggcgttt aaactctccg
    15541 acccggagaa attgctgggt ggcgcagaag atttaggtat tacctgtgcg ctgatcccaa
    15601 ccaccacgcc gggcgtggaa attggtcgtc gccacttccc gctgaacgtg ccgttccaga
    15661 atggaccgac gcgcggtaaa gatgtcttcg taccgatcga ttacattatc ggcgggccga
    15721 aaatggccgg gcaaggctgg cggatgctgg tggagtgcct ctcggtaggc cgcggcatca
    15781 ccctgccttc caactcaacc ggcggcgtga aatcagtagc gctggcaacg ggtgcgtatg
    15841 cgcacattcg tcgtcagttc aaaatctcta ttggtaagat ggaagggatt gaagagccgc
    15901 tggcgcgtat tgccgggaac gcttatgtaa tggatgctgc ggcatcgctg attacctacg
    15961 gcattatgct cggcgaaaaa cctgccgtgc tgtcggctat cgttaagtat cactgtactc
    16021 accgcgggca gcagtcgatt attgatgcga tggatatcac tggtggcaaa ggcattatgc
    16081 tcgggcaaag caacttcctg gcgcgtgctt accagggcgc accgattgcc atcaccgttg
    16141 aaggggctaa cattctgacc cgcagcatga tgatcttcgg acaaggagcg attcgttgcc
    16201 atccgtacgt gctggaagag atggaagcgg cgaagaacaa tgacgtcaac gcgttcgata
    16261 aactgttgtt caaacatatc ggtcacgtcg gtagcaacaa agttcgcagc ttctggctgg
    16321 gcctgacgcg cggtttaacc agcagcacgc caaccggcga tgccactaaa cgctactatc
    16381 agcacctgaa ccgcctgagc gccaacctcg ccctgctttc tgatgtctcg atggcagtgc
    16441 tgggcggcag cctgaaacgt cgtgagcgta tctcggcacg tctgggtgat attttaagcc
    16501 agctttacct cgcctctgcc gttctgaagc gttatgacga cgaaggccgt aatgaagccg
    16561 acctgccgct ggtgcactgg ggcgtacagg atgcgctgta tcaggcagaa caggcgatgg
    16621 acgatttact gcaaaacttc ccgaaccgcg tggttgccgg gctgctgaat gtggtgatct
    16681 tcccgaccgg acgtcattat ctggcaccgt ctgacaagct ggatcataag gtggcgaaga
    16741 ttttacaagt gccgaacgcc actcgctcac gtattggtcg gggtcagtac ctgacgccga
    16801 gcgagcataa tccggttggt ttactggaag aggcgttggt ggatgtgatt gccgccgacc
    16861 cgattcatca gcggatctgt aaagagctgg gtaaaaatct gccatttacc cgtctggatg
    16921 aactggcgca caacgcgctg gcgaaggggc tgattgataa agatgaagcc gctattctgg
    16981 tgaaagcgga agaaagccgt ctgtgcagta ttaacgttga tgactttgat ccggaagagc
    17041 tggcgacgaa gccggtaaag ttgccggaga aagtgcggaa agttgaagcc gcgtaattga
    17101 ataacggagc cgaaaggctc cgtttcttta tccgctaatt atttaaaatt aaagccatcc
    17161 ggatggtttt ccaggctgcc ggtcaacgcc gcgaacagca ccgttttacc atcaatcgaa
    17221 agcgcatcgt tcacattcag ccaggtgagt ttctcttgcg acgttttctc atcaatagtc
    17281 gagaacagcc ccgtcatctg attagatttc tcggaccaca tcacagcgat acgttgcgag
    17341 ccacagtcat gcggtttgca cgcgctcatc acctgatacg tctcatctcc caacgttacg
    17401 gtttgtgcgg gagtataagt accgcctttc atcacccatg caggtagctt atgcccttgc
    17461 accatctgat taaacgcagc tttggtggtt tcgccctttg ccaggctgct aatggttaaa
    17521 tcatcctgcg ccattgcact ggtggcgatg accagagcgg cgactgtcgt tattgcctta
    17581 aacatcattc ctcccgagct tatcctgccc atgttattaa cctcctaaag cctggcgata
    17641 acacgataat ttgcaaatca gatatttcca atatcagcat ttgttatcaa tttattctgc
    17701 gctaaggcaa ctgcctgata aggtgttaaa gttataaaac ccttcgcata aaggagttgt
    17761 acaccgtgcc tggtttgaag attacgcttt tgcagcaacc actggtgtgg atggatggcc
    17821 ctgccaacct gcgtcatttt gatcgtcaac tggaaggtat taccgggcgc gatgtgatcg
    17881 ttctaccgga gatgtttacc agcggctttg ccatggaagc ggcagcttcg tcgctagcac
    17941 aaaatgacgt agtgaactgg atgacagcta aagcgcagca gtgcaatgcg ctgattgcag
    18001 gcagtgttgc attacaaacg gagtctggtt cggttaaccg ctttttgctg gttgagccgg
    18061 gcggcacggt acatttttat gataagcgtc atctgttccg catggcagat gagcatctgc
    18121 attataaagc gggcaatgcg cgagtgattg tggaatggcg cggctggcgt attttgccgc
    18181 tggtgtgcta cgacttacgt tttcctgtgt ggtcgcgcaa tctcaacgat tatgacctcg
    18241 ccatctacgt tgccaactgg cctgctccgc gctctctgca ctggcaggca ttgctgacgg
    18301 cccgcgcgat tgagaatcag gcgtatgtgg cgggatgcaa tcgcgtcggc agcgatggca
    18361 acggctgcca ttatcgcggt gacagccggg tgattaaccc gcagggcgag attatcgcta
    18421 ctgccgacgc gcatcaggca acgcgcattg atgcggagct gtcgatggtg gcattgcggg
    18481 aatatagaga aaagtttccg gcatggcagg atgcggatga gtttaggttg cggtgagtgg
    18541 gggttgtgtt taaggctggg ggagagtcgg ggtaagacta cgaaagcccg ctctccgcaa
    18601 ggactgacgc cagatagttt ctgtccatag ctgcttttcg catcttacgt cttaaccctg
    18661 ccttgaatac cttatcattc gtcaaaatat taatagcgat gtgccgtatc cctgaaaata
    18721 attctgcggc gtttcctctt cttatt
//
LOCUS       DAPHJE010000088        18584 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPHJE010000088 DAPHJE010000000
VERSION     DAPHJE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.088"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            147..1367
                     /locus_tag="RH222_003143"
     CDS             147..1367
                     /locus_tag="RH222_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3798126.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1340..1969
                     /locus_tag="RH222_003144"
     CDS             1340..1969
                     /locus_tag="RH222_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV3798127.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1970..3130)
                     /gene="ybdL"
                     /locus_tag="RH222_003145"
     CDS             complement(1970..3130)
                     /gene="ybdL"
                     /locus_tag="RH222_003145"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV3798128.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3239..4327
                     /gene="hcxA"
                     /locus_tag="RH222_003146"
     CDS             3239..4327
                     /gene="hcxA"
                     /locus_tag="RH222_003146"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV3798129.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4337..4534)
                     /locus_tag="RH222_003147"
     CDS             complement(4337..4534)
                     /locus_tag="RH222_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV3798130.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4716..6821)
                     /gene="cstA"
                     /locus_tag="RH222_003148"
     CDS             complement(4716..6821)
                     /gene="cstA"
                     /locus_tag="RH222_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV3798131.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7002..7415)
                     /gene="entH"
                     /locus_tag="RH222_003149"
     CDS             complement(7002..7415)
                     /gene="entH"
                     /locus_tag="RH222_003149"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV3798132.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7418..8164)
                     /gene="entA"
                     /locus_tag="RH222_003150"
     CDS             complement(7418..8164)
                     /gene="entA"
                     /locus_tag="RH222_003150"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV3798133.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8164..9021)
                     /gene="entB"
                     /locus_tag="RH222_003151"
     CDS             complement(8164..9021)
                     /gene="entB"
                     /locus_tag="RH222_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV3798134.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9035..10645)
                     /gene="entE"
                     /locus_tag="RH222_003152"
     CDS             complement(9035..10645)
                     /gene="entE"
                     /locus_tag="RH222_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV3798135.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10655..11830)
                     /gene="entC"
                     /locus_tag="RH222_003153"
     CDS             complement(10655..11830)
                     /gene="entC"
                     /locus_tag="RH222_003153"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV3798136.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12205..13161
                     /gene="fepB"
                     /locus_tag="RH222_003154"
     CDS             12205..13161
                     /gene="fepB"
                     /locus_tag="RH222_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV3798137.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13165..14415)
                     /gene="entS"
                     /locus_tag="RH222_003155"
     CDS             complement(13165..14415)
                     /gene="entS"
                     /locus_tag="RH222_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV3798138.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14526..15530
                     /gene="fepD"
                     /locus_tag="RH222_003156"
     CDS             14526..15530
                     /gene="fepD"
                     /locus_tag="RH222_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV3798139.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLIAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15527..16519
                     /gene="fepG"
                     /locus_tag="RH222_003157"
     CDS             15527..16519
                     /gene="fepG"
                     /locus_tag="RH222_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV3798140.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16516..17331
                     /gene="fepC"
                     /locus_tag="RH222_003158"
     CDS             16516..17331
                     /gene="fepC"
                     /locus_tag="RH222_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3798141.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17328..18461)
                     /gene="fepE"
                     /locus_tag="RH222_003159"
     CDS             complement(17328..18461)
                     /gene="fepE"
                     /locus_tag="RH222_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV3798142.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4367 a         5263 c         4795 g         4159 t
ORIGIN      
        1 tatataacta cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa
       61 taaaacactc tgctttacag tgtttacttt ataattaaca gtcttatctc actgttcacc
      121 ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata
      181 ttctggaagc tgcacgagag cgtattacct ggacactcaa caccctaccc cgcgtttgtg
      241 tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc gaacttgccc
      301 gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca
      361 ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact
      421 gggttgcgct cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt
      481 gctgggaacc tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg
      541 actttttctg cttttaccag ccaggcatga ccttcgaaca atttgtacgt gagtttgccg
      601 aatggttttc acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg gatgagtcct
      661 acaaccgttt tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccct
      721 ggactacggc cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag
      781 tggctgatat ctggacctgg tatgcaaatc atcaaagtct ctgcaaccca ctgtataacc
      841 taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg
      901 agcaacgaca aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt
      961 gcgcacgagt cagcggggta aaaagtggcg gcatttacgc cggacatgac aatcatttct
     1021 atgggcatcg gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc
     1081 tgctcaatag catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt
     1141 tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg
     1201 cgaaagatat cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt
     1261 gtcgggcatt atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga
     1321 taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac
     1381 gcaatttctc gccagcttgc cggaagatga tcgcatcaac gcgattaatg aaatccgcat
     1441 ggcgatccat caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa
     1501 aaacagccaa cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa
     1561 actgctgaaa aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacgcatac
     1621 ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc
     1681 atcatcatta aaattacgct taaaaggtta tttgccggtg acctgtctgg aaggtacgcg
     1741 taatcaacgc atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac
     1801 cgccatgtcg gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg
     1861 caaagagctg ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggtctgca
     1921 agagctgttt gccgaccgtc aatattcccg cgcctggact gtcaaataac tacagctggc
     1981 gcagtcgttc agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca
     2041 gtttatgagg gaagggatcg gcgcaaaaca ccgatagcgg aatcgccgct acgccgtgct
     2101 cctgcgtcag ccactggcaa aactcaacat catccagggt agatacggcg ctgtaatcca
     2161 ccagcaaaaa gtatgtacct tcacacggta aaatctccag ccggctttca tttaaagcat
     2221 tcaccagaat atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt
     2281 ctgcacgtag catatcagca agcgccagct gtgccggggt attcaccgaa aaggtcagat
     2341 actgatgtac cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa taacccactt
     2401 tccagccggt catatgatag gtcttgccaa atgaagaaac cgccactgcc cgctcacgca
     2461 gctgcggatg cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga
     2521 cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat
     2581 cagcctgctg ccagacagtt gcactggggt tatgcggagt gttgaggatc accagtctgg
     2641 tgcgctcgct taacaatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca
     2701 gtgccatacg cttcactatt cccccagaaa gcgcgatggc gggggagtaa ctgtcatagc
     2761 tgggatcaaa acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata
     2821 acgcttccgt cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat
     2881 acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg
     2941 cgtattggtt tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac
     3001 catcaaaatc aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc
     3061 tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt
     3121 tatttgtcat ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc
     3181 tagtatttgg caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat
     3241 gcctcacaat cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag
     3301 tttcaatcac ctgcacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta
     3361 cggcaaacgc gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg
     3421 ggcaaagcat attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc
     3481 tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac
     3541 cgcgaaagcc ctcgcccgcc gtctcggtct gccatttgtt gccgctccga cgatcgccgc
     3601 cacctgcgca gcctggacac cgctctccgt ctggtataac gatgccggac aggcgctgca
     3661 ttatgagatt ttcgacgacg ccaattttat ggtgctggtg gaaccggaga ttatcctcaa
     3721 tgcaccgcaa caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc
     3781 ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc gtgcggctgg ggatcaataa
     3841 tgcgcaggcc attcgcgacg tcttgttaaa cagtagcgaa caggcacttg ccgatcagca
     3901 aaatcagcag ttaacgcaat cattttgcga tgtggtggat gctattattg ctggtggtgg
     3961 gatggtcggt ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa
     4021 cggtctgact gtcctgccgc aaaccgagaa gtttcttcac ggcaccaaag tcgcttacgg
     4081 aattctggtg caaagcgcct tgctgggtca ggatgatgtg ctggcgcaat taaccggggc
     4141 gtatcagcgt tttcatctgc cgactacgct ggcggagctg gaagtggata tcaataatca
     4201 ggcggagatc gacaaagtga ttgcccacac cttgcgcccg gtggagtcca ttcattacct
     4261 gccagtcacg ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa
     4321 agcctgacgt gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct
     4381 gccgctcacg gaaaaactcc tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca
     4441 tatgttcgac atagttgtcg taatcaggca taccaatcat cagcttcgcc gcctgaccta
     4501 aatattttcc ggcttttgcc agtgaatcaa acatcttggc ctccgctaac catgttgctg
     4561 aaagagtgcc ggatgcggcg tgaacgcctt atccggccta caaatgctat caacgcgctc
     4621 ggtcagtccc ctcgccccat cggggagagg gttagggtga ggaggaaccc tcggcactat
     4681 gccaacattc gccaacatcc cccctcactc tgactttagt gtgcgccttt tgcctgcgcc
     4741 acgatctcct cgacattttc cggcattggc tcatacggcg tttctttcgc cgttggcttc
     4801 ggatctttca atgccgccag tgccgtctta atcgagaaca gcgccagaac caccacgacc
     4861 accataaaga agatggttaa cccggcatcc agacggttgt tgaacaccag ttgcgccagc
     4921 tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttatta
     4981 gcaatggcca ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gcctgcggtc
     5041 agggtacaaa tcagcagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt
     5101 ttcatcttga acaacaccac ggcacagagc atcagcgcca tccctgccag catctggttg
     5161 gcaataccaa acagcggcca cagagtgtta atgccgccca gcggatcgac cacgccctga
     5221 tggaggaagt agccccacgc cagtacgcac agcgctgttg ccagcaggtt agcaggcagt
     5281 gaatcggtcc gcttcaggcc cggagaaacc acgcccagca gatcctgcaa cataaagcgc
     5341 gcagcacgcg tacccgcatc taccgccgtc agaataaaca gagcttcaaa cagaatggcg
     5401 aagtgatacc agaacgccac atccatcata ccacccagcg caccgtgcag gatgtaagcc
     5461 atccccaccg ccagcgtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact
     5521 tcgctggcaa tctggttgag cgtatctggt gtaatgctaa agccccagct actcaccacc
     5581 tgcgcggcag aagcgactac atccgccgtc cctgccggag ccagcaccgc cattgggctg
     5641 ttcatggcaa aatacacgcc cggatcgatg atacaggcag aaaccagcgc cataatcgcc
     5701 acgaaggatt ccatcaacat cccaccgtag ccgataaagc acgcctgccc ttcgttcgcc
     5761 agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga agccagacac cgcaccacag
     5821 gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc agtgccatcg
     5881 acaaatttgg tcagcgcagg catggtcagc gtcgggcgca taatcaaaat gcctaccgcc
     5941 agaccgacga tagtcccgat tttcaggaag gtcgagagat agtcacgcgg ggccagcagc
     6001 aaccacaccg gcagcaccgc cgccacaaaa ccgtagccca ccagcatcca ggtcagctgc
     6061 acgccggtaa agtcaaagta cggtgcccag gtcggacttt ctgccaccca gccgccagag
     6121 ataatggcga aaatcaggaa caccaggcca atcactgaca cttcgccaat gcggcccgga
     6181 cgcagatagc gcaggtagat tcccataaaa atcgccagcg gaatggtgaa cgcaacggta
     6241 tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg
     6301 ataatgacca tgatcataaa gcaggctacc agcgcaatca ccccggcagt tggccccatc
     6361 tcttctttga ccagctcacc cagcgatcga ccgtcacggc gcgtagaaac aaacagcacc
     6421 atgaaatcct gcaccgcacc agcgagaacc accccggcga gcagccagat catccctggc
     6481 aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc ggcaatggcc
     6541 gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta
     6601 tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac
     6661 agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc
     6721 gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca
     6781 gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt
     6841 gtccatagag atgttacatt ttgtttctat aacatctttg tacgatttaa caacatggta
     6901 accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg
     6961 ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta
     7021 cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat ttcccagctc
     7081 tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc
     7141 gggcgatgat gggtggcgtt aagttccgta cccaccacac actgcccgtc acgggtcatc
     7201 ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc
     7261 aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag cacatcatct
     7321 cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc
     7381 agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc
     7441 catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa
     7501 tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact
     7561 gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt
     7621 gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg ctacccgcca
     7681 gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac
     7741 tcataccaat acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgcccc
     7801 gctgacggcg aaactggttc atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa
     7861 ccgcaaaagt ctgctgccag tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa
     7921 ttcccgccgc attgatcagc acgtccagtc gctccgtttc agctaacagt cgctgacata
     7981 cctgcgccac ctgcccggcg tcggcaacat ccattacttc ggtcgcaaag ggatattgct
     8041 cctgagtgaa cgcttgatca aaacctgtaa ctttcgctcc cgcctcaaca aacgccagcg
     8101 ccgtggcgta gccgatacct ttacctgcgc cggttaccca gacattttta ccgctgaaat
     8161 ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc
     8221 cagcatgaca aagtcgatat caccatgcac tttgcgccag cgcgccgcca gcgccatcat
     8281 gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc
     8341 gtccagcaac ggcaggataa cctcacgcag cgccgctttg ctggcgggga caggtgctgg
     8401 cagtaattct tcagtcatca ccacccggcc agaccgcccg gccacatatt tcagcgacat
     8461 caaatgctcg tcacggctga aatcggccag cgcatccgcc accataaacg gtttaatatc
     8521 gcgcataaat gcgtcggttg cggtggtcat acagccaatg tgggcatata ccccggtaat
     8581 aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac gatgaaacgc
     8641 gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac
     8701 cttttgctgt tccggcgaac gggtcaggcc cggcccccac atatcattca acagcgcccg
     8761 atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accgggatat tgtgctgttt
     8821 gcagtagtcg cgcagcgcag caatattcgc gatcacctgt tccatcatcg ggcagttctc
     8881 gccccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg
     8941 ttcaaaggcc cagtcaactt tattctgcgg aatatcgtga gactccggca gtgcgtaagc
     9001 ctgtaatttt ggaatagcca tagtgttctc tccttcaggc tgatgcgcgt gacgccagcc
     9061 actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatccacac
     9121 actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca
     9181 cctgcaccgc gcgcagcggc tcttttacca ccagataagc gcagcttttt tcgcccatca
     9241 gctcatcttc catgctcacc agtgcggcat agatcactgc cggatggcgc agcagcaggt
     9301 tttcgatctc ttcggcagcg atcttctcgc cgccacggtt gatctgatct ttctcgcgcc
     9361 cctgcacggt gatgtaaccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac
     9421 cgttggcatc aaaggcgctg gcattgtgct gcggactttt gtaataaccg cggaaggtgt
     9481 acggcccgcg cgtcatcagg cgtccgactt ccccttgcgg cagtggattt ccttcggcat
     9541 cggcaaccca cacttcatcg tccggacaca ttgggtaacc ctgggtatgg ataattttct
     9601 ccgcgctatc atcaagacgg gtgtagttca ccagcccttc cgccatgcca aacacctgct
     9661 gcaactgaca gccaatctca gcgggaatac gcgccgcaag ggtggcagaa agacgtgcgc
     9721 cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcgcct tcggtcagcg
     9781 cctgcaacca caggctgact gcgggtggca ccagcgcggt aacgttaacc tgatgttttt
     9841 caatcaatgg gaaacagagc gtggcgctgg gatcggccgc cagaacaacc gttcctccgg
     9901 caagaaagac gcccagcgat cccggcgaac tcatggcgta gttatgagcc gccgggatcg
     9961 cgcacaggta gcgtgtctgt tgtgtgaact gacaaatctc gacgctacga cgcacgctgt
    10021 agtagtagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa
    10081 gctggaaata ggccacttca tcagcaggtg atggcgtggc agtaaaatcc tcagccggat
    10141 ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa
    10201 tggaggaatg ttctgtgacg aaagtattga ggaaatcatc cccgctaaac agcgcatgtt
    10261 ggcgatcggc aatcagcaat gcgggttcaa tctggctggc ataggcgttc agttcactac
    10321 gctgatggct gaacagcgcc agcaccggcg caacgcccag tttcagcagc gcgaaaaagg
    10381 taatatacaa ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca ggtttaatgc
    10441 cctgacggcg taaactacac gcgaggttat ccgccgcctg attcagctcc cggtaactca
    10501 actgtcgctc gccgtcgata accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt
    10561 cggtcagcgg caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg
    10621 gccagcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa acgttcaaca
    10681 tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa
    10741 tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca
    10801 ccacccattc gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc
    10861 ggtcgaatgg ctccagttca gcaataacct gggtcgcggc ctgatgcggg aaaccgctca
    10921 gcgcgggggt tggatgcagc agacaggcca gagtcagtgc gttttcttgc gaattcgctt
    10981 taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg
    11041 agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca
    11101 gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga
    11161 gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc
    11221 cgtctttacg tagcagcagt tccgggctgg cccccagcag gacgccacca tcagccagcg
    11281 ggacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac
    11341 tatcaatggc ggcgtcagtg gtgatatcaa tcaatcgtga caacaccact ttgtcgacct
    11401 gcggcgtggc ggtaagtgcg gcggcgcgag caaccatctg ttcaaacgtg gtttgttcag
    11461 gaattgcctg gcgttccacc acgttcagcg actggctgcg ggtaaaacgg cgggctgagg
    11521 tctgtttttc ctggcgggag aacgactgcc aggattcggg aatatacagc gacgaaggct
    11581 gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc tgcgctttgg
    11641 catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa tccccgttca
    11701 cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca
    11761 taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg
    11821 acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat
    11881 tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct
    11941 gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt attgatggat
    12001 tcgcataagc gcaatgtgat gtcctgcgcc gttctgcccc ctctcccttc cagggtgagg
    12061 gctggggtga gggttaatgt tcgcaccagt gctggctgtt cccctcaccc taaccctctc
    12121 cccaaagggg cgaggggacg gattgtgcgc tttgtcgaat ttgtcattac gcccttaacc
    12181 ttattaataa caggaagctg atttgtgaga ctcgccccgc tctaccgcaa cgcccttcta
    12241 ttaacaggac ttttgctttc aggaatagcc gcagttcagg ccgctgactg gccgcgtcag
    12301 attactgaca gccgtggcac acatacactg gaaagccagc cgcagcgtat tgtttccacc
    12361 agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc cagcggcgcg
    12421 accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg gagcaaggtg
    12481 gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcgccga agccgttgcc
    12541 gcgcaaatgc cggatctgat tttaattagc gcaaccggcg gggattcggc gctggcactg
    12601 tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga caaaagctgg
    12661 cagtcgctgt taacgcagct tggcgaaatt accgggcatg agaaacaagc ggcagagcgg
    12721 attgcacagt ttgataagca actggcagcg gcgaaagagc aaatcaaatt accgccgcag
    12781 ccggtcactg ccattgtcta taccgccgct gcacacagtg ccaatctctg gacgccggaa
    12841 tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt gcccgcaggg
    12901 ttaaacgcca gccaaagcca gggcaaacgc catgacatca ttcagcttgg tggggaaaat
    12961 ctggctgctg ggttaaatgg cgagtcacta ttcctgttcg ccggtgatca gaaagatgcc
    13021 gatgcgattt atgccaatcc actgctcgcg caccttcccg cagtacaaaa caagcaggtt
    13081 tatgcgctgg gaaccgagac gttccgtctg gattactaca gcgccacgca agtgctggaa
    13141 cgacttaagg cgctgtttta agcattaact gtcagacgct gtcacctgcg gcggcgtctg
    13201 gcgaaaacgt cgcaactcca ccagcaccag caataacaac acgccgataa tcaacaaacc
    13261 aaaaccgctt gcgcttgcgg aagcaaccgg tgtcatcatc gcgcccaaac cacccagcag
    13321 cgccgcgcct atggcatcgc ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc
    13381 taacatcgct tccggggttt gcgtttgcag cattgtatat tgcagcaacg aactaaccgc
    13441 gctcagccag ccgaacagcg ccagacaaac cacgcctaaa atccacatcg gcatcaggcc
    13501 aaacagacca atggcgagga acgatcccag cgtggagagc agcatcaata accctggtcg
    13561 cgcactatgt gccagcttcc cgctggttaa cgcaccaata gccgcgccga gcgggatcgc
    13621 cgcgtagaga aaaccaatct gtgccgctga catctgccag ttgtcagcca gcgccggata
    13681 cagtacccgc accgcgctcg ccatcgtcaa taaaccaccc agcagcgcaa tcccgcccac
    13741 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcacg
    13801 cggctgcggt ggcggtggca acgccggaag gcttaacaac ggtagcaagg taataaacgt
    13861 gcccgccgcc gccagcccgt agttccaggc tacgccaccg gtcgccagca ataaaccgcc
    13921 aatcatgggc gaaatcaccg accccagacg cacggtcaac atggtgatcg ccccggcctg
    13981 cattaagttt tcacgcccta ccagtgctgg tgtcgccgcc aatagcgccg taacgccaag
    14041 cgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    14101 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    14161 aatcactttt ttgcgctcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    14221 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gcgtcatcat
    14281 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    14341 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    14401 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    14461 gcacatgcta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    14521 ttcgtatgtc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    14581 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    14641 tagtgctgga ggccctctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    14701 cgcgactgcc gcgcaccctt gccggtttaa ttgcaggcgg cgcgcttggc cttgccgggg
    14761 cgttgatgca aaccctcacc cgaaatccac ttgccgaccc cggcttgctt ggcgtgaatg
    14821 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcgcagg
    14881 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    14941 gcagtcaggg cggcgggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    15001 cggcggtgct ggaagggctg accagcggca tcgccctgct taatcctgat gtctacgatc
    15061 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    15121 tgctgatccc ggtgctgatc gccggagcaa ccgcgctatt actgagccgc gcgctgaaca
    15181 gtttaagcct cggcagcgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    15241 taattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    15301 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    15361 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    15421 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    15481 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggcgcatga tttacgtctc
    15541 tcgccgatta atcatcacct gtttgctgtt ggtttccgcc tgtgtggttg caggtatctg
    15601 gggattacgc agcggtgccg tcacgctgga aacctcgcag gtattcgccg cgctgatggg
    15661 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    15721 ggcgctgttg attggcgcgg cactgggcgt cagcggcgcg atttttcagt cgctgatgcg
    15781 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    15841 ggtagcgatg gtgctgtttg gtcaggacct gacggctatc gcgctggcag caatggtggg
    15901 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggca tcgacacctt
    15961 tcggttgatt attatcggta tcggcgttcg tgccatgctg gtggccttta atacctggct
    16021 gttgctgaaa gcgtctttag aaacggcgct aacagcgggt ttgtggaatg caggatcgct
    16081 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    16141 tgccgccgcc ttactggtac gccggatgcg cttgctggaa atgggcgatg ataccgcgtg
    16201 tgcgctgggc gtcagcgtcg aacgttcgcg tctgttaatg atgctggttg cagtggtgct
    16261 taccgctgcg gcaacagcgc ttgccgggcc gatttccttt attgctttag tcgcaccgca
    16321 cattgcccga cgcattagcg gcaccgctcg ctgggggcta acccaggcgg cgctatgcgg
    16381 ggcgctgtta ctgctggcgg ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact
    16441 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    16501 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    16561 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaatacctga tggtcacttc
    16621 acggcaatta tcgggccaaa tggctgcggt aaatccacgt tactgcgtac cttaagccgc
    16681 ctgatgacgc ctgctcatgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    16741 agtaaagagg ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    16801 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    16861 tggcgcaaag aggatgaaga agcggtaacg aaagcaatgc aggccacggg aataactcat
    16921 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    16981 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    17041 atcagtcatc agattgattt gctggagttg ttaagcgaac tgaaccgcga aaaaggctat
    17101 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    17161 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    17221 ctgattgagc gcatttatgg tctgcgctgc atgatcattg acgatccagt tgccggaacg
    17281 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    17341 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaacag cacgccacca
    17401 caagccacca tcccgccgat caacgcggaa aggatcacaa taatcgcctt acccggaccg
    17461 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    17521 ttgacatgtg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    17581 agttccgcaa cgtcagtgac cgctttttct atttccagtt tgcgttcaat accgtctgca
    17641 ccgagagaaa tagaaaaatc ggggtcatct ttaacggcct gaccattact gtaaacgggc
    17701 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    17761 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    17821 ttgatctcca gtttattacg gacgttttct agcgactctt tcaccaccaa cgtagagata
    17881 taatcgatat acccgctcaa aacggtctgc gcctcttcac tggttggcgc ggtaaaactt
    17941 agcgtccagg aggtatacag tgacggttca tcttttttct tactggcatt gtcatcaacc
    18001 gctttcattt tttcgctcaa tgcgacaatt gcgcgatgca aatccagttc gtcgattttc
    18061 gcctctttta attggtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    18121 accgactgaa acttcttgat aaacaggtta aatgcttctg tacgatcaat tttgatatcc
    18181 agatccagca ctcgaagctt agtgaatgtt ttctccagct cttgccactg aacaggttct
    18241 ggaggcgtga caaccgccgc gctggtccat ttttgcggca ggatgaaaga gatcagcaag
    18301 cctgcgcagg caaacgcaaa aatgaccgcc atgaccgttt ttttggcccg ccataaaact
    18361 gcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagga
    18421 aaatgagcgt cacttccctg tttaatattc agtgatgaca tggggtataa cctgaaaatg
    18481 agtccaatag gggaaatctc cacggcgctc actttaccag ttggtgataa ttatccgaag
    18541 ctgaagtttg taaattcctt ccactgaatg caacattcac tcta
//
LOCUS       DAPHJE010000089        18571 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPHJE010000089 DAPHJE010000000
VERSION     DAPHJE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.089"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..884
                     /locus_tag="RH222_003160"
     CDS             <1..884
                     /locus_tag="RH222_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDV3798143.1"
                     /translation="PMPRHANPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYIKEVF
                     VSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSYENDELTYTPGAD
                     DNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERPVKL
                     MIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVKATLLSSR
                     YLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDTADRLNYQ
                     KMAQVVDGVINLLYNSK"
     gene            987..1490
                     /locus_tag="RH222_003161"
     CDS             987..1490
                     /locus_tag="RH222_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV3798144.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1557..3014)
                     /gene="nuoN"
                     /locus_tag="RH222_003162"
     CDS             complement(1557..3014)
                     /gene="nuoN"
                     /locus_tag="RH222_003162"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV3798145.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYVPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3021..4550)
                     /gene="nuoM"
                     /locus_tag="RH222_003163"
     CDS             complement(3021..4550)
                     /gene="nuoM"
                     /locus_tag="RH222_003163"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV3798146.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4714..6555)
                     /gene="nuoL"
                     /locus_tag="RH222_003164"
     CDS             complement(4714..6555)
                     /gene="nuoL"
                     /locus_tag="RH222_003164"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV3798147.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6552..6854)
                     /gene="nuoK"
                     /locus_tag="RH222_003165"
     CDS             complement(6552..6854)
                     /gene="nuoK"
                     /locus_tag="RH222_003165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV3798148.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6851..7405)
                     /gene="nuoJ"
                     /locus_tag="RH222_003166"
     CDS             complement(6851..7405)
                     /gene="nuoJ"
                     /locus_tag="RH222_003166"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV3798149.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERHWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNPKDDSAKRKTEEHA"
     gene            complement(7417..7959)
                     /gene="nuoI"
                     /locus_tag="RH222_003167"
     CDS             complement(7417..7959)
                     /gene="nuoI"
                     /locus_tag="RH222_003167"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV3798150.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7974..8951)
                     /gene="nuoH"
                     /locus_tag="RH222_003168"
     CDS             complement(7974..8951)
                     /gene="nuoH"
                     /locus_tag="RH222_003168"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV3798151.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8948..11674)
                     /gene="nuoG"
                     /locus_tag="RH222_003169"
     CDS             complement(8948..11674)
                     /gene="nuoG"
                     /locus_tag="RH222_003169"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV3798152.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQEHLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11727..13064)
                     /gene="nuoF"
                     /locus_tag="RH222_003170"
     CDS             complement(11727..13064)
                     /gene="nuoF"
                     /locus_tag="RH222_003170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV3798153.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13061..13561)
                     /gene="nuoE"
                     /locus_tag="RH222_003171"
     CDS             complement(13061..13561)
                     /gene="nuoE"
                     /locus_tag="RH222_003171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV3798154.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13564..15366)
                     /gene="nuoC"
                     /locus_tag="RH222_003172"
     CDS             complement(13564..15366)
                     /gene="nuoC"
                     /locus_tag="RH222_003172"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV3798155.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15460..16122)
                     /gene="nuoB"
                     /locus_tag="RH222_003173"
     CDS             complement(15460..16122)
                     /gene="nuoB"
                     /locus_tag="RH222_003173"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV3798156.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16138..16581)
                     /gene="nuoA"
                     /locus_tag="RH222_003174"
     CDS             complement(16138..16581)
                     /gene="nuoA"
                     /locus_tag="RH222_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV3798157.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17212..18150)
                     /gene="lrhA"
                     /locus_tag="RH222_003175"
     CDS             complement(17212..18150)
                     /gene="lrhA"
                     /locus_tag="RH222_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV3798158.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4639 a         5143 c         4803 g         3986 t
ORIGIN      
        1 cgccaatgcc ccgacacgca aatcctgagc aattagaaaa aacagttcgt tatcttacac
       61 aaactgtgca tccacgtagt gccgacaata ttgataatct gaataggtcc gcagagtata
      121 taaaagaggt cttcgtcagt agcggtgcca gaattacctc gcaggacgtc cccattacgg
      181 gtggccccta caaaaacatt gttgctgatt atggtcctgc cgatggaccg ctgattatta
      241 ttggtgcgca ctatgactct gccagcagtt atgaaaacga tgaattgacc tatacacctg
      301 gcgcggatga taacgccagc ggcgtggcag gattactcga actggcacgt ttgttacatc
      361 agcaagtacc gaaaacaggc gtgcagctgg tcgcctatgc gtcggaagaa ccgcccttct
      421 ttcgtagcga tgaaatgggg agcgcggtac atgcagcttc gcttgagcgt ccagtaaaat
      481 taatgatagc actggagatg attggctatt acgactctgc gcctggtagc caggattacc
      541 cttacccggc aatgtcctgg ctttatcccg atcggggaga ctttattgcc gtggtcggca
      601 gaatgcagga tatcaacgcc gttcgtcagg taaaagcgac attgttatca tcccggtatt
      661 tatctgttta ttctatgaat gccccagggt ttattcccgg tattgatttc tctgatcacc
      721 ttaattattg gcaacacgat attcccgccg taatgattac tgacaccgct ttttatcgta
      781 ataaacaata ccacttgccc ggtgataccg cagacagatt gaattatcag aaaatggctc
      841 aggtggtgga tggtgttata aatttgttat acaacagtaa ataataaaaa tttctcgagg
      901 cgggtccaga aaaagcgaca tgcacatttt attttccctc gtctgtttta agatgcgaaa
      961 catgaaattc aatgaaggaa aaataaatgg gaatgattgg ttattttgca gaaattgact
     1021 cagaaaagat taaccaactg cttgagtcta ctgaaaaacc tttgatggat aatatacatg
     1081 acacgctttc aggattacgc cgactggata ttgataaacg ctgggatttt ttacactttg
     1141 ggctgacagg cacctctgct tttgatcccg ccaagaatga tccgctgagt cgtgccgtgc
     1201 ttggtgaaca cagcctcgaa gatggcatag atggcttcct gggattgacc tggaatcagg
     1261 agttagccgc gacaatcgac cgactggaat cactggatcg aagtgagctg cgtaaacagt
     1321 tttccattaa gcgactcaat gaaatggaga tttatccggg agtgaccttt tcagaagagt
     1381 tagaggggca actctttgcc tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc
     1441 gacgtatgct gcgacaaggg aatcatgcgc tgacggttat tgttgggtga tggtgtcaga
     1501 gcgccaaaaa aaaccgccga gcatgtcggc ggctttctga cttacaaact aacagattac
     1561 atcagcggca ttgctaaccg cacaatgcta atcagcggtt gtggccatac acccagcacc
     1621 agtaccaaca gtgcagaaat cagcaccact ataccgcccg cgctgtactg ccagtttgat
     1681 ggtgcatcgc gacccggttg ttccggggcg tgaagataca ggctcaccgc cacgcgcagg
     1741 tagtagtaga ggccgattgc cgaaccgaca accacggcac ccaccagcca ccacaagtgt
     1801 gcctggacac cgaccgccag cacgtagaac ttaccgataa agcccagcgt catcgggata
     1861 ccggccagag acagcatcat caccgtcatc actgccgcga ggatcggacg atgccagaac
     1921 agaccgcggt aagagaacag ggaatcagca tccgggccac gatacgggct ggacatcagg
     1981 ctgaccacgc cgaacgcgcc gaggctgctg aacagataac cggccaggta aacccctacc
     2041 gcttccatcg acatctcacc agtttgcagc gcaatcagcg ccaccagcag atagccgagg
     2101 tgagagatag atgagtaacc gagcagacgt ttgatgttgg tctggctcag cgccatcagg
     2161 ttaccgaaga tgatggaggc aaaggcgata atcgccagta ctacgcgaat cgcttcgctg
     2221 tcacccaccg gtacgtagag gaacagacgc atcaccacac cgaagatagc gattttgctc
     2281 gccgtcgcca ggaaagtgga aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac
     2341 aggtggaacg gcaccagcga gagtttgaag ccgaggccaa caatcatcag tccgaaacct
     2401 gccagcaaca gcggctcgtt gagcataccg tcgccgaggt ttttacccag cgcgacaaac
     2461 gacaggtcgc cagactgtgc atacaccagc gccataccaa acagcaggaa agaagacgct
     2521 gcggcagaaa ggatggtgta tttgatactg gcttccagcg aacgtttctg gcggaaagcg
     2581 taaccgacca ggccaaacag cggcaaagag atcagttcaa taccgaggaa caaagacgcc
     2641 agatggttgg cattcgccag caggatcccg cccagcgcgg caattaacac caacaggtag
     2701 aactcatcct tgttgtcgtt atagccttca agccacgggt aggcgaaagt acaggtggcg
     2761 aggctcgcca acaataccag cccggtgtaa agcatggcga aaccatcaac gcgcatcagc
     2821 ggcgtaacgt ccatagcgcc cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta
     2881 agcccaataa ccgagagcgt agcgttgagg aaatgattgc gtcgccacgc aatggagagc
     2941 atcacaacca ccaccgtcaa gccgacgatc agcaacggta gcagtgcgat caggttttgt
     3001 ggagttattg tcatggcgat ttacggcctt gtagtagtaa cggaattaac aaaccactgc
     3061 tggatattgc caatcgcgga gtgcgaggta tccagaatcg gctgcggata gaagcccagc
     3121 agtaccagca gcaccaccag caacaggatc ataaacagct cacgcagcga catccctggc
     3181 agttcctggc tggcaatctg gcttttcgct ttaccgaagt aagcgcgatg caacatcgcc
     3241 agcgaataaa cagatgcaaa gaccagccca aaggtagaga taacggtaat caccgggaca
     3301 acctggaagc tgccgaacag aatcataaat tcgccgacga agttaccggt gccaggcatc
     3361 ccaagcgttg ccaccgcaaa gaacagcgac agtgctggca gccatttcat cttgctccac
     3421 agaccgccca tcatgcgcat gtcgcgggta tggatacgtt cataaagctg accacaaaga
     3481 ataaacagac ccgccgccga caagccgtgc gcaatcatct ggattaccgc gccctggtag
     3541 gccaactggc tgccggtgta gatagcaatc agcacgaagc ccatgtggga aaccgaggtg
     3601 taggcgatca gacgtttgat atcggtctgg gcgaaagcca tccacgcacc gtagaagatg
     3661 ccgataaccc ccagccacat agcgattggc gcaaactctg ccgatgcgtt cgggaacagc
     3721 ggcagggaga aacgcagcaa accgtaagcg gcagttttca gcaagatccc cgcgaggtca
     3781 acggaaccgg cggtcggagc ctgagagtgc gcatccggta gccagccatg cagcggaacc
     3841 accggcattt tgactgcgaa ggcgatgaag aagcccagca ttaacaggta ttccacacca
     3901 ctggacattg gcgtattcag cagctcttca tagttgaagg tccagacgcc ggtcgcattg
     3961 tagtgaacaa aaaccagcgc caggatggca atcaacatca ccaggccact cgcctgggtg
     4021 taaatgaaga acttggttgc cgccgtgata cgcgttttac cgtcagaggc tttatgcccc
     4081 cacagtgcga tcaggaagta catcggcacc agcatcattt cccagaagaa gaagaacagg
     4141 aacatgtcga tggcaaggaa cacgccgata acgccgccca ggatccacat caggttgagg
     4201 tggaagaagc cctgatattt ttcgatctct ttccacgaac atagtaccgc cagcacaccg
     4261 agcagaccgg tcagcacgac catcagcagc gacagcccgt caatggcgag atgaatagag
     4321 ataccaaaac gcgggatcca cggcatgtcg aattcagact gccactgcgg aattccggcg
     4381 gattgcgtca gtgaataacc gccctgcaac cacagttgca gcgacagcgc cagcgtcaat
     4441 cccatggtga tcagcgcgat ccagcgtggc accttgacgc caaagcgttc ggtctgccag
     4501 cacaggaagc cgccaataaa gggaattaat attagccagg gtagtaacat ggcgatcttt
     4561 attccttgta aaagtcccgt caggaccgga ttttcaacga attctcacga cagaaaattc
     4621 tcttcggact gggggtagtg tcggatgcac cgcgaaggcg gcttatccga cctacgttct
     4681 tatatgccca ggggcaatcc cacaatcctt aactcaacgc agtaccatca acagtgccag
     4741 caccacgacc gcaccgatgc tcatggatgc cacataccag cgcagataac cgttctcgct
     4801 taacagcaga cctttacctg caaagcggga aagaaccgcc gggatgttca tcattgagtt
     4861 cagcggatcg cgtttcagca accaggcaat acccaggaac ggtttgacga acactttgtc
     4921 gtacagccag tcaaagcccc aggcgttgta ccaccaggtg cccagcagac ggcccggcgc
     4981 actgttggca atggaggtca ccagagtacg tttacccagc cacagccagg ctgccagcag
     5041 aatgccgacc accgcgacca cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc
     5101 cagctccgtc gtttgcggaa gcacgccctg cagcggcggt acaatcagtg cgccaacgaa
     5161 ggtggaaagg atcagcagca caatcagcgg caggctgtga gttacccctt tcacggcgtg
     5221 agcgtgaatt tgttcttttc cgtggaagac gatgaaaatc atacggaagg tgtagagcga
     5281 ggtcataaac gcaccgacca gacctgccac catcagattg atatgaccat tcgccatcgc
     5341 acccgcgagg atctcatcct tactgaagaa gccagcagtg accagcggca gcgccgacag
     5401 tgctgcgccg cctaccagga agcagagata aaccagcgga atagatttac gcagaccgcc
     5461 catcttgaag atgttctgtt cgtgatggca ggccagaatg acggaaccgg atgccaggaa
     5521 cagcagcgct ttaaagaacg cgtgagtcat caagtggaaa atcgccgcat cccatgcctg
     5581 cacgccaagc gcgaggaaca tgtagccaat ctggctcatg gtagagtaag cgagaacacg
     5641 tttgatgtct gtctgtacca gcgcggcaaa accggccagc agcagcgtaa ccgccccgac
     5701 aatacccacc agatgcagaa cttccggggt catcaggaac aggccgtggg tacgggcgat
     5761 caggtagaca cccgcggtta ccatcgttgc agcgtggatc agcgcggaga caggcgtcgg
     5821 gccagccatc gcatcggcaa gccatgtctg caacggcaac tgcgcagatt taccgaccgc
     5881 accgcccagc agcatcagcg tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc
     5941 tggtgccagt tccaccattt cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa
     6001 cagtgcgaaa gcgaggaaca cgtcacccac acgggtcacg acgaacgctt tcattgccgc
     6061 tgcgccattc ttcggatcgg tgtaatagaa accgatcagc agataggagc acaggcccac
     6121 gccttcccag ccgaggtaca tcagcagcag gttgtcggca agcaccagaa ctaccatgct
     6181 ggcgatgaac aggttggtgt aggcgaagaa gcgagagtag ccctcttcac cgcgcatgta
     6241 ccaggaggcg tacatgtgaa taaggaaacc cacaccagtg accaccgaga gcatggtcag
     6301 cgacaggccg tccagcacca gattgaaacc gatgttaaag tcgcctaccg acatccacgt
     6361 ccacagcggc tggctgtatg actgctcgcc gttagcgaag aaatcaacgc cgataaaggc
     6421 ggttaccagc gccgccaggc ccacagagcc aacgccgacg atcgccgaaa cgttttcaga
     6481 ccagcgccca cgggagaacg ccagcaggac gaagccaatc aatggcaaaa taatggttaa
     6541 ggcaagcatg ttcatccgcg catctcactt actgaatcga tgttcaggtt ctggcgacga
     6601 cggtgaagtt gcagcagcag cgcaaggccg atactcgctt ctgccgccgc gaggctgatg
     6661 gcgagaatgt acatcacctg accgtcggtc tgaccccagt agcttccggc gaccacaaag
     6721 gccagcgcgg aggcgttaat catgatttcc agaccaatca gcataaacag cagattgcga
     6781 cggataacca gaccggttaa gccaagaacg aataagattg ccgcgaggat cagtccatgt
     6841 tgtaagggga tcatgcgtgc tcctccgttt ttcttttcgc gctgtcgtct ttaggattgc
     6901 tcagcacttc acccgcacgc tcttcacgac cgacgtggaa ggccacaacc agacccgcga
     6961 gcagcagcat agaagccagt tccaccgcca gtacgtaagg cccgaacagc gtaataccca
     7021 ctgctttagc actgattggc gtaccgtcga taccctgatc attaacacca aggatggcgt
     7081 aaacaatcac caccagcatg atggccgaca aaattgccgg accaatccac acctgcggtt
     7141 tcagccaatg gcgttcctgt tcgatttctg aaccgcccag gttgagcatc atcaccacga
     7201 acacgaacag caccataatg gcacccgcgt agacgataat ttccagcgca ccggcgaagt
     7261 aagcgcccag tgagaagaac accccggaaa tcgccagcag cgaaataatc aggtacagca
     7321 gtgcgtgtac cggattggta tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc
     7381 cacagatata aaaagcgaac tccattgccc ctctccttac ggtaacaggc tcttgacgtc
     7441 gataggcttg gcttcgttct ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc
     7501 catccggtag aagttatatt ccgggtattt gcccggaccg gagatcagca gatcctcttt
     7561 ttcgtaaacc agatcctggc gcttgtattc ccccatttcg aaatccgggg ttaactgaat
     7621 cgccgtggtc ggacaggctt cttcgcacag accacagaaa atgcagcgtg agaagttgat
     7681 gcggaaaaat tccgggtacc agcgaccgtc tttggtttct gctttttgca gcgagataca
     7741 gccgaccggg caggctaccg cgcagaggtt acaggctacg caacgctctt cgccgtccgg
     7801 gtcgcgggtc agaacgatac gaccacgata acggggcggc agatagaccg gctcttccgg
     7861 gtacattcgc gtttcgcgtt tggcgaacgc gtgcaggccg atcatccaga tactacgaac
     7921 ctgggtgccg aaacctacta acaattcttt taaggtcatg gtcttattgc cccttattgc
     7981 gcctgccaga gaatgacagc cgccgttacc agcaagttga tcagcgtcag cggcaggcag
     8041 attttccagc cgaaggacat tacctggtca taacgcggac gcggtaacga cgcacgaatc
     8101 aaaatgaaca tcatcataaa gaacgcggtt ttcagcgcga accagatgaa tggcggtaac
     8161 aacgggcctt gccagccacc gaagaacagc gtcaccatca atgcagagat ggtcacaatc
     8221 ccgatgtact cacccacgaa gaacagaccg aacttcatac cggaatattc aatgtggtaa
     8281 ccatccgcca gttcctgctc ggcttccggc tggtcaaacg ggtgacggtg acataccgcc
     8341 acgcccgcga tggcaaaggt aataaaacca aagaattgcg ggataacgtt ccacacatgc
     8401 gcctggctgt tgacgatgtc ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc
     8461 agggaaagcc cgaggaacac ttcgtagctc agggtctgcg cagaagcacg catcgcaccc
     8521 agcaacgagt atttgttgtt acttgaccag cccgcaaaca gcaccgcgta aaccgccaga
     8581 cctgccatca tcaggaagaa caaaatcccg atgttcaggt cggcaaccac ccaacccgga
     8641 ctgactggca caatcgcaaa ggccagcagc agcgaggtaa aggcaatcat cggtgccagg
     8701 gtaaagatga cgcgatccga gaatttcggg atccagtctt ctttaaagaa cattttgatc
     8761 atgtccgcaa ccagctggag cgaaccgccc cagccaacac ggttaggtcc gtaacggttc
     8821 tggaacagac ccagcaggcg acgttcgcca aagctcatga atgccccgca ggtgacaacc
     8881 accagcagga tcaccaccgc tttgaggatg gtcagcagga tctcaatcag ttccggtgat
     8941 atccaactca ttgttgtgcc tccttgagat cctcaagatg cgcgccagcc agcaccggag
     9001 caataccgga catacccatc ggcaagccca cctgccctgc cgtcagccct tcagcgattt
     9061 caaccggcag cgtgaccgtg ttgccatcgt aactaaagga gacgtgtgta cctgcgttca
     9121 cacctaactt cgcggcatcc gctgggttga gtttgatgta cggctgcggc atacggctct
     9181 ggaagaccgg agcacgctgt gacaattcat cgctgccaaa caggtggtaa tacggcgcga
     9241 tacgccattt cccgtcctgc ggctggaagc gtgccggtac gctggtgaag taatccagac
     9301 cattttcgct ggtttcaaac agacgcacgc ccggatcgcc aaagcgcagt ttgccgccca
     9361 cttcgtcctg gaatttgttc cacgcctgcg gggagttcca gcccggcgcc caggcaaacg
     9421 gcacttgcga acggtgcgca gtcggctggt tgttaccttc catcgagaag gtgaacatgg
     9481 tgtcaatatc ctgcggctga cgcggctcat gaacgctgat attggcgcgc atggcggtac
     9541 gaccgctgta acggtgcggt tcacgggcca gtttctgccc acgaatacgg aatgtcgcat
     9601 ccggcgcagc atctttgata cctgccagtt ccgggatttt cgccacaaca gcgtcaatca
     9661 catggtcgag ctgcgtccag tccacttcac ggctcagcag ggtgctgtgc agcgagtgta
     9721 accagcgcca gctttccagc atgacagttt tgctgtcgta ataagcagga tcgtaaacct
     9781 ggaagaaacg ttgggcgcgg ccttcgttgt tgatcaccgt accgtcgctt tcagcaaagc
     9841 tggcagcaga aagtaccaga tgggcgtttt ccataatcgc tgtgcgttga tgatcaacca
     9901 ccatcaccag cggcgcttta gccagcgcag cattcacgcg ggtagcggaa gcgtgacgat
     9961 gcagatcgtt ttccagcacc accaccgcgt cggcgcgtcc ggtttccagt tcggtcaacg
    10021 cttcttcaag cgaaccgcca cccataatgc ccagccccat gctgttgacg gaacgggcaa
    10081 tcatggtgat accaacgtca gcgccgcgac ctttcagggc tttcgcgacg ttagccgcag
    10141 cctgaatcac ctctatgcta ccggcgttcg tcccggagat aatcaacggt ttcttcgcac
    10201 ctgccagtgc ctgcacgatg acgtcgattt tgctttgcag ctcaggttcg ataccgtcaa
    10261 ccgctggcgc agagttatcc agcgcatggg cgatggcaaa acctaaacgc gcctgatctt
    10321 caaccggtgc gcggtaagtc cacgccgcga tatcatccag acgggtgtca tcaacgttgg
    10381 taacaaacag cggatgcttc gcacgttgac cgatgttgag gattgccgca atctgccagt
    10441 cagccacttt ctgtgctgcc gccatttcgc gcgctttacc tttcacagcc tgacgcactg
    10501 ccagcgcgac gcgcgcgccg gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt
    10561 aagattcgat ttcgcgcaga gccggagtat aaatgccgcc ttcacgcagc actttcagcg
    10621 ccagttgcag atgttcctgc tcaccgtgag cgataccggt gtagaagttt tcttcgccca
    10681 ccagttcacg cagcgcaaag ttgctttcca cgctggcacg cggagaacca ataccgatca
    10741 ctttcttcga ctgacgcaga atatctgccg cgccctgcat tgcctgttcg gcgttgaggg
    10801 taatgaaatc atcgccacga cgctgtactg gttgacgcgg acgatctttc aggttgacgt
    10861 aaccgtaacc gaaacgacca cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac
    10921 ggttttcgat acgacgcagt tcgccgtaac gttcaccggg gctgatgtta cagccgatgg
    10981 aacattgctg gcagatgctc ggcgcaaact gcatatccca tttacggttg taacgctcgg
    11041 agtgcgtttt gtcggtaaat acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt
    11101 cgctttccag cgtgccgtct tccgggcgac cgaagtagac gttgtcgtgc gcaccgtaaa
    11161 cgcccagatc cgtaccgtca gcgtaatctt tgtagtaacg cacgcagcgg tagcaggcga
    11221 tgcagcggtt catttcgtga gagatgaatg gccccaaatc ctgattacgg tgggtacgtt
    11281 tggtgaaacg gtagcgacgg aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc
    11341 agttaccgcc ttcttcacat accggacagt cgtgcgggtg gttggtcatt aaccactcga
    11401 ccacactttc acggaactgt ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg
    11461 aagccggtgt catacaggac atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt
    11521 gcttcaccgc acactggcgg caagcaccga cgcttcccag cgccggatgc cagcaaaagt
    11581 aaggaatatc aaggcccaga gacagacaag cttccagcag gttgtccgct ccgttgacct
    11641 cgtattcttt gccgtctaca tgaattgtag ccattagcat gcttccagtt ttctcggtca
    11701 gggaccgagc gttaatcgaa attcggttac cagcgctctt tcagcaggtt cggctgaatc
    11761 ccattaatca aatgggtatt gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg
    11821 aaatatttga tggcgctctg taacggctcc actgcaccag gtgcgtgggc acagaaagtt
    11881 ttacccgggc ctaagaatcg acacagttgc tcaagtgttt cgatatcgcc cggctgacct
    11941 tcaccacgct ccagcgcacg cagaattttc acgctccacg gcagaccgtc gcggcacggc
    12001 gtacaccagc cgcaggactc acgggcgaaa aactcttcca ggttacgcac cagcgacacc
    12061 atgttgatct catggtcaac cgccatcgcc agcgccgtac ccagacggct tcccgcttta
    12121 ccgatacttt cgaattccat cggcaggtca aggtgcgctt cggtcaggaa gtcggtgccc
    12181 gcgccgcctg gctgccaggc tttgaatttc agaccgtcac gcataccacc ggcgtaatct
    12241 tcgaggatct cgcgcgcggt agtaccaaac ggcagttccc acagtcccgg atttttcacc
    12301 cgaccggaga agcccatcag cttggtgcca gcatctttac ttttcgagat gttctgatac
    12361 cactccacgc cgttagcgag gatcgccgga acgttacaca gggtttcgac gttgttgaca
    12421 caggtcggtt tgccccatac gccggaggtt gccgggaagg ggggtttcga gcgtgggtta
    12481 gcacgacgcc cttccaggga gttgattaac gctgtttctt ccccgcagat gtagcgccct
    12541 gccccggtat ggacgaacag ttcgaagtcg aaacctgttc ccataatgtt tttgccaagc
    12601 aagcccgctt cagtggcttc ggcaatggcg cggcgcagat ttactgccgc ttcgatatat
    12661 tcgccacgca ggaagatgta gccacggtaa gctttcagcg caaacgcgga gatgagcata
    12721 ccttccacca gcaggtgcgg cagttgctcc atcagcaggc ggtctttata ggtgcctggc
    12781 tccatttcat cggcgttaca cagcaggtaa cggatgttca tggattcgtc tttcggcatc
    12841 aggctccatt tcaggccggt agaaaagccc gcgccaccgc gccctttcag accagcgtct
    12901 tttacctgat taacgatttc gtccggagac agcccggtca gcgccttacg cgcgccttcg
    12961 taaccgtttt tgctgcgata ttcgtccagc cacactggct gtttgtcatc gcgcagacgc
    13021 caggtcagcg gatgcgtttc gggagtacgg ataatgtttt tcatttatac cgctccagca
    13081 gttcagggat cgcttccggg gtcagatgcg cgtgagtatc ctcatcgatc atcatgtttg
    13141 gccctttatc acagttcccc aggcagcaag ttggaagcag cgtaaagcga ccgtcaaatg
    13201 tcgtttgccc tggtttgatg tttagctttt tctcgagcgc cgcctgaata ccctggtaac
    13261 cgttgatatg acagaccacg ctgtcgcaat aacggatcac atggcggcca accggctggc
    13321 ggaagatctg gctgtagaac gttgccacac cttcgacgtc gcttgccgga atacccagca
    13381 catcggcgat cgcgtggatc gcaccatccg gcacccagcc acgctgcttc tgaacgattt
    13441 tcagcgcttc aatggacgcc gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg
    13501 cttcacgctc tgccgcactc agctcaaaag cctcggtttg tggttgttga ttctcgtgca
    13561 taattagcgg tccacatctg acataacaaa atcgatactg cccagataaa caatcaggtc
    13621 agacaccagg ctgccgcgga tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt
    13681 gcggatacga gtgcgataac tcatggtgct accgtcgctg gtcaggtagt aactgttgat
    13741 ccccttggtc gcctcaatca tctggaaaga ttcattggca ggcatcaccg gcccccacga
    13801 cacttgcagg aagtgggtga tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg
    13861 cggcgtggtc agcgggtgat ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg
    13921 ctcaagaatg cgcagactct ggcgcagctc ttccactttc agcattacgc gggtgtagca
    13981 gtcagaaaca ccaccaccaa ccgggatttc aaagtcgaag ttttcatagc cagaataagg
    14041 acgcgcctta cgcacgtcga agtcgatccc ggtagcacgc aggcccgcgc cagtggtgcc
    14101 ccactccagc gcctctttcg cgccataggc ggcaacgccc tgggaacgac ctttcagaat
    14161 ggtattttgc agcgccgctt tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa
    14221 ctcacgcagc aggcgatccc agccgcgcgg caggtcgtgc gctacgccgc caatacggaa
    14281 ccacgccggg tgcatacgga aacccgtgat cgcttccacc agatcgtaaa ttttctgacg
    14341 atcggtaaag gcgaagaaca cgggcgtcat tgcgccgacg tcctgaataa aggtcgagat
    14401 gtacagcagg tggctgttga tacggaacag ttcggagagc ataacgcgaa tgacgttaac
    14461 gcgatccggc acggtgatcc cggccagttt ctctaccgcc agcacgtaag gcatttcgtt
    14521 aacgcagccg ccgaggtatt caatacggtc ggtatacgga atgtagctgt gccaggactg
    14581 gcgctcgccc attttctccg caccacggtg gtggtagccg atgtctggta cgcagtcgac
    14641 aatctcttcg ccgtcgagtt gtaaaacgat acggaaagcc ccgtgcgccg acgggtggtt
    14701 cggaccgagg ttgaggaaca tgaagtcctc gttttcggtg ccgcgcttca tcccccactc
    14761 ttccggtttg aaggtcaggg cttccatctc cagatcctgt ttggctttgg tcagctcaaa
    14821 cggcgagaat tcggtagcgc gcgccgggta atctttacgc agcgggtgac ctttccaggt
    14881 ttgcggcatc atgatgcggc gcaggttcgg gtgaccgtcg aaagtaatgc caaacagatc
    14941 ccaggtttca cgctcatacc agttagcgtt cgggaacagt ttggtgaagg tcggtacgtg
    15001 caggtcgttt tctgccagcg ccaccttcag catgatgtcg cggttacgat cgatagaaat
    15061 cagatggtag aaaacggaaa aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg
    15121 ttcgtccatg ccgtgtaagt caaacagcat gacgtaaggt ttcggcagtt tctttaagaa
    15181 atcgccaact tccagtaatt gttcacgctt gatccacaca acgggaaccc cggtgcgagt
    15241 cgcctgaaca gtaaaggcat ccggcccaaa acggttgcgc agttcgccaa tcaccggatc
    15301 atcaagatga tcgcgggtct gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt
    15361 caccattgca aatggtccgt ggtgactgtc ggcggagtaa gcttcgcgaa atagatttag
    15421 gaataagcga agaaaaatcc ctttgccgac aggcgcaaat taaatctcgt caggtgtacg
    15481 caggttggtt acggcaatgc gttcaccgcg cttgcgttcg cgctctgatt gcatattggc
    15541 gcgataaacg ccctgatcgc caaccaccca ggagagcgga cgacgttctt tgccgataga
    15601 ttcctgcaac agcatcagtg cctgcatgta cgcttcagga cgcggcgggc agcccgggat
    15661 atacacatca accgggatga atttatcgac gccctgcaca acggaataaa tatcgtacat
    15721 accgccggag ttggcacagg cacccattga gataacccat tttggttcca gcatctggtc
    15781 atacagacgc tgaataaccg gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag
    15841 gtcagcctga cgcggcgaag cacgcaatac ttctgcgcca aaacgcgcca cgtcatgcac
    15901 cgcggtaaac gaagtcacca tctcaacgta acagcaggaa agaccgaagt tatacggcca
    15961 aattgagttt ttacgacccc agttaaccat gtcattgagc ttgcccataa acacgttttt
    16021 gttaacttct tgctccagag ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc
    16081 gttctcaccg ttgggatcta tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta
    16141 gcgttgacga ttagcgatac tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc
    16201 gggcgtccag tccagcgcgc caatacgcac cagataaacc agacctgcca gtaacacaaa
    16261 aataaaaatt gcagcttcca caaagcctac ccagccgctt tcgcggatag aggttgacca
    16321 tgcgaacaga tacagcgctt caacgtcgaa gataacgaag aacatggcca ccagataaaa
    16381 cttggcggac aggcgtaagc gggcggagcc gaccgagtcg ataccggatt caaacggcac
    16441 gtttttcgac ctcgcgcgtg cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca
    16501 acacaggcca atggcaacga taagaaagat agcgaatgcc cagtgatgag cgatgacttc
    16561 agtggatgtt gacatactca ttgcttactc atcaaaagta gcgccagatt cactgctctt
    16621 cacggcagat ggacgccaca tcgattcatg gggaggaata aaaaaaacct tacaatcact
    16681 gtagaaattc ttttatacag ctaattgatg tggtctttta ctcctttcta taaccttttg
    16741 tcaactttaa caaaagtttc ttcacattag tttacataat atcaacacca ttagcattta
    16801 atgccctttc accccagatc cttgacgact ccaggataat tagatgttgt tgaatcgtgt
    16861 ccgttgtgaa gcaatggaaa aaatacgggt ctattttgac aggaatttgt gtcgattcct
    16921 ccccccaaaa gagagtattt tcttgatctg tgacacgctt ttgtcattcc ataacaaaaa
    16981 cgcagcaaca aatttacgta ttttttaaca tcattgtagc aggtgatttt tttcaggcga
    17041 ttatttgtgc gttcgggacg tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt
    17101 ctgcactatg cgaggaagga tttttactaa aaaaaagccg ctggggttta aaacaccccc
    17161 agcggctcgt tttttacact attgtctcag gaattatcta tcgtccgtcg attactcgat
    17221 atccctttca atcaacaatg aatcatcccc ttccggagca gacattgggc tgtattgcca
    17281 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    17341 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
    17401 cagcacacgc aggtccgggc tcatcatctc aaccggcctt gccgtcacgc caagaccggc
    17461 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccatggaat
    17521 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
    17581 tacaagaggg atcggctcgc ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    17641 tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
    17701 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    17761 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    17821 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    17881 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
    17941 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    18001 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    18061 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    18121 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    18181 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    18241 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    18301 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
    18361 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
    18421 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    18481 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    18541 aaccttttat ttataagggt cattgcgaat t
//
LOCUS       DAPHJE010000090        18486 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPHJE010000090 DAPHJE010000000
VERSION     DAPHJE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.090"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(56..1321)
                     /locus_tag="RH222_003176"
                     /pseudo
     CDS             complement(56..1321)
                     /locus_tag="RH222_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1507..1591)
                     /locus_tag="RH222_003177"
     tRNA            complement(1507..1591)
                     /locus_tag="RH222_003177"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1555..1557),aa:Leu,seq:caa)
     gene            1787..2806
                     /gene="ahr"
                     /locus_tag="RH222_003178"
     CDS             1787..2806
                     /gene="ahr"
                     /locus_tag="RH222_003178"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV3798159.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADY"
     gene            complement(2810..3373)
                     /gene="idnK"
                     /locus_tag="RH222_003179"
     CDS             complement(2810..3373)
                     /gene="idnK"
                     /locus_tag="RH222_003179"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV3798160.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            3590..4621
                     /gene="idnD"
                     /locus_tag="RH222_003180"
     CDS             3590..4621
                     /gene="idnD"
                     /locus_tag="RH222_003180"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HDV3798161.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            4645..5409
                     /gene="idnO"
                     /locus_tag="RH222_003181"
     CDS             4645..5409
                     /gene="idnO"
                     /locus_tag="RH222_003181"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDV3798162.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            5472..6791
                     /gene="idnT"
                     /locus_tag="RH222_003182"
     CDS             5472..6791
                     /gene="idnT"
                     /locus_tag="RH222_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HDV3798163.1"
                     /translation="MPLIIIAAGVALLLILMIVFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6858..7856
                     /gene="idnR"
                     /locus_tag="RH222_003183"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6858..7856
                     /gene="idnR"
                     /locus_tag="RH222_003183"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HDV3798164.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7934..9436
                     /locus_tag="RH222_003184"
     CDS             7934..9436
                     /locus_tag="RH222_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV3798165.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTVSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9597..10679)
                     /gene="lptG"
                     /locus_tag="RH222_003185"
     CDS             complement(9597..10679)
                     /gene="lptG"
                     /locus_tag="RH222_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV3798166.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(10679..11779)
                     /gene="lptF"
                     /locus_tag="RH222_003186"
     CDS             complement(10679..11779)
                     /gene="lptF"
                     /locus_tag="RH222_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV3798167.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            12046..13557
                     /gene="pepA"
                     /locus_tag="RH222_003187"
     CDS             12046..13557
                     /gene="pepA"
                     /locus_tag="RH222_003187"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV3798168.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13911..14354
                     /gene="holC"
                     /locus_tag="RH222_003188"
     CDS             13911..14354
                     /gene="holC"
                     /locus_tag="RH222_003188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV3798169.1"
                     /translation="MKNATFYLLDNDTTIDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            14354..17209
                     /gene="valS"
                     /locus_tag="RH222_003189"
     CDS             14354..17209
                     /gene="valS"
                     /locus_tag="RH222_003189"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV3798170.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELERLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(17263..18459)
                     /locus_tag="RH222_003190"
     CDS             complement(17263..18459)
                     /locus_tag="RH222_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDV3798171.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNVYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4725 a         4599 c         4701 g         4461 t
ORIGIN      
        1 ttttttaaca aagaaaataa tcctagaata attcccgggc atttgcccgg gatgattact
       61 gttttaatgg attattaatc tttgcatatt caaatggact gataaacctt tctctgttta
      121 catccagaaa atcggcccac cactgtagca tcaatcgccg ttcttccaga tgctctgctt
      181 tatggatata cgcggccctc actgaatttc gcgccatgtg gctcatctga cgttcaacag
      241 catcacgaga ccacagacct gattcgacca atgaactaca ggccattgtt cgaaagccat
      301 gaccacaaac ctctactttt gtatcatacc ccatgacccg taacgcacta tttaccgtat
      361 tctcactcat gggtttgtgc gaatcgtgat caccaataaa tatcaagtca tgggccccat
      421 aaaactgttt tatctgcttt aaaattgcaa gagcttgcgt tgaaagaggc actagatgcg
      481 ttgtacgcat ttttgagcct ctatgggaat gtttcactcc aggaataggc tcccgctccg
      541 gtgggatagt ccatatagac gcttcgaaat cgatctctga ccaacgagca aaacgcagct
      601 cactggaccg aataaagatc agcaaagtga gttctatcgc ccatcgggtt agcggcctac
      661 cagtatagct atctattttt gtaagcaact cagggatgcg ctttaattca agcgcgggac
      721 gatgttgtcg attacaggaa gcaaccgccc cagccatctc ttgtgccggg ttataatcaa
      781 ttaacccact ttgcactgca tagcgcatga tggctgtagt gcgctgctga agacgagcgg
      841 ccacttcaag acgtccagac atttctacgg ccttaatagg tgctaataaa tctcgagttt
      901 ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag attatcttca agacttttta
      961 gcacacgatg ggcgtgatct tcagaccact ttttattggt gccatgccac tcaatcgcga
     1021 cttctttaaa ggttcgtgct ttactctgtt caaccttatc atttttcttt ttgtctcccg
     1081 gatcgacgcc attcgcaagc agcttacgcg tctcgtcacg acgtactctg gcatccgcta
     1141 gtgtgatttc aggataaacc ccaagtgcca gcattttttg ctttccctca taacggtact
     1201 gcaaacgcca gtacttagaa ccatttggat ggacaagcag atgcataccg ccaccatcag
     1261 tcagctaata ttgcttatcc gttggcttgg ctgctctgac tttgatatct gttaatgcca
     1321 tcgtattccc tccctgttgg tacaagcatt atcgaaccgg aaataccatc atctgtacca
     1381 acatatgttg gtagatgtac gttgatatcg gttgaccttg agagagttaa tatagcggga
     1441 aagatagata aacactggat ttcaggcata aaaaaagacc tcagttgagg tctatttaca
     1501 tacttttggt gccgaaggcc ggactcgaac cggcacgtat ttctacggtt gattttgaat
     1561 caactgcgtc taccgatttc gccacttcgg cactgaagag gtatgcggaa aacgttgtgg
     1621 attatacctg tcgcacgcca ccatgcaagc gccagcttgc tcgcacctcg ctaagtgctg
     1681 aaattttcag cattgcatac agcgatgtgt aacctttgtc acactccagg caccccgccc
     1741 tgccatgctc tacacttccc aaacaacacc agagaaggac caaaaaatgt cgatgataaa
     1801 aagctatgcc gcaaaagaag cgggcggaga actggaagtt tatgagtacg atcccggtga
     1861 gctgaggcca caagatgttg aagtgcaggt ggattactgc gggatctgcc attccgatct
     1921 gtcgatgatc gataacgaat ggggattttc acaatatccg ctggttgccg ggcatgaggt
     1981 gattgggcgc gtggtggcac tcgggagcgc cgcgcaggat aaaggtttgc aggtcggtca
     2041 gcgtgtcggg attggctgga cggcgcgtag ctgtggtcac tgcgacgcct gtattagcgg
     2101 taatcagatc aactgcgagc aaggtgcggt gccgacgatt atgaatcgcg gtggctttgc
     2161 cgagaagttg cgtgcggact ggcaatgggt gattccactg ccagaaaata ttgatatcga
     2221 gtccgccggg ccgctgttgt gcggcggtat cacggtcttt aaaccactgt tgatgcacca
     2281 tatcactgct accagccgcg ttggggtaat tggtattggc gggctggggc atatcgctat
     2341 aaaacttctg cacgcaatgg gatgcgaggt gacagccttt agttctaatc cggcgaaaga
     2401 gcaggaagtg ctggcgatgg gtgccgataa agtggtgaat agccgcgatc cgcaggcact
     2461 gaaagcactg gcggggcagt ttgatctcat tatcaacacc gtcaacgtca gcctcgactg
     2521 gcagccctat tttgaggcgc tgacctatgg cggtaatttc catacggtcg gtgcggttct
     2581 cacgccgctg tctgttccgg cctttacgtt aattgcgggc gatcgcagcg tctctggttc
     2641 tgctaccggc acgccttatg agctgcgtaa gctgatgcgt tttgccgccc gcagcaaggt
     2701 tgcgccgacc accgaactgt tcccgatgtc gaaaattaac gacgccatcc agcatgtgcg
     2761 cgacggtaag gcgcgttacc gcgtggtgtt gaaagccgat tattgaaaat cattcgcagc
     2821 gctgatctga ggcgctgccc tctttcgcac atattctgtt ttgtcgtatc gccagcacag
     2881 cctgccgaca ttgttcggtg acattggcaa tatcatggtt gatatcaatg cgcacaatat
     2941 cctgttcatc tgcttgtgga cgctccagtg cctcaaactg actttttagt aacgctaccg
     3001 gcataaaatg cccagcccga cgctgcattc gcgcgagaat agtttcatag tcgccatcta
     3061 accagaggaa atgaacatgg gggctaccct tgcgtaaaat atcacgatac tgttttttta
     3121 atgatgaaca gacaataaat cctgtttcat tctttttata aagactgtat gaagcatcat
     3181 ttaagcgttc cagccaggga agtcgatctt catcagataa tggaataccc tgcgacattt
     3241 tatctatatt tttggctgga tgaagatcgt caccatcaat aaatttagca gataataacg
     3301 cggcaacctt gctaccaatt aatgttttac cactccctga aacgcccatc aaaataaagc
     3361 tttcacccgc catttaaaat ctcctgaatc atcttacctg ctgcgatact acgcctggcg
     3421 gcataataat ttcacgtttt tttatttcta ctgataagaa ttacaaggca catcacgtta
     3481 tgcgtaacat agtaatgtaa caattttctg acgtgatctt catcacaaat aatgacagtt
     3541 aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt gaggaattta tgcaagtgaa
     3601 aacacagtcc tgcgttgttg cgggcaagaa aactgttgcc gttaccgagc agacgataga
     3661 ttggaataat aatggaacat tagtacaaat aacccgaggt ggaatttgcg gttccgattt
     3721 acattattat caggaaggaa aagtaggtaa tttcatgata aaggcaccga tggtgttagg
     3781 tcatgaagtt atcggtaaag ttattcatag cgactcatca gaattacatg aagggcaaac
     3841 ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa tactgcattg aacataacga
     3901 gaatcagtgt acagatatgc gtttttttgg cagtgccatg tatttccctc atgttgatgg
     3961 tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt gtcccttatc cggccaaagc
     4021 tgacgaaaag gttatggctt ttgccgaacc tttagccgtc gcgattcatg ccgcacatca
     4081 ggccggcgag ttacagggca agcgagtatt tatttccggt gttggaccca ttggctgcct
     4141 gattgtcagt gcagtgaaaa cactgggggc cgcggaaatt gtctgtgctg atgtgagtcc
     4201 ccgttccctt tcgctgggca aagagatggg ggcggatgtg ctcgtaaacc cacaaaacga
     4261 cgacatggat cactggaaag cggaaaaagg ctatttcgat gtcagctttg aagtgtccgg
     4321 tcatccttca tcagtgaata cctgtctgga ggtcactcgt gcacgcggcg taatggtgca
     4381 ggtaggtatg ggaggcgcga tggcagaatt cccaatgatg acgttgattg gtaaggagat
     4441 ttcactcaga ggctctttcc gttttaccag cgaatttaat accgcagtgt catggctggc
     4501 gaatggcgtt atcaatccac tgcctttact gagtgctgaa tatcccttca ctgacctgga
     4561 agaggcgcta cgtttcgccg gtgataaaac ccaggcagca aaagtccagc ttgttttcta
     4621 agagataaat aaaggaataa tacaatgaac gatctatttt cactggcagg aaaaaatatc
     4681 ttgattaccg gttcagcaca gggcattggc tttttactgg caaccggcct gggtaaatat
     4741 ggcgcacaaa taattattaa tgatattact gccgaacgcg cagaacttgc tgtagaaaaa
     4801 ctccaccagg agggtattca ggccgttgcc gcacctttta atgttactca taaacatgaa
     4861 attgatgccg ccgttgaaca tatcgaaaag gacatcggcc ccattgatgt gctggtgaat
     4921 aacgccggta tccagcgccg tcatcctttt actgagttcc ctgaacaaga gtggaatgat
     4981 gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc aagcggtaac tcgtcacatg
     5041 gttgaacgca aggcaggtaa agttattaat atttgctcga tgcaaagcga actgggacgt
     5101 gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa aaatgctcac ccgcggcatg
     5161 tgcgtcgagc tggcgcgcca caatattcag gtcaacggta ttgcgccggg ctatttcaaa
     5221 acagaaatga ctaaagcact ggttgaggac gaagccttca ccgcctggtt gtgcaaacgg
     5281 acccccgccg cacgctgggg agatccgcag gaactgattg gtgctgcggt gttcctttct
     5341 tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg ttgatggcgg catgttagtg
     5401 gctgtttaac agccccccct acgtgcagcg cgaaagcgct gcttttctgg cactaataag
     5461 agataaagac tatgccatta atcattattg cggcaggcgt cgcgctgctt cttatcctga
     5521 tgatcgtctt taaagttaac ggctttattg ccctcgttct ggtagctgcc gtcgtcggat
     5581 ttgccgaagg gatggatgca caggccgtcc tgcactctat acaaaatggt atcggcagca
     5641 cgctcggcgg gctggcaatg atcctcggtt tcggggccat gttaggcaag ctgatttctg
     5701 atacgggtgc ggcacaacgt atcgccacta cgctgattgc tacttttggt aaaaaacgcg
     5761 tgcaatgggc gctagtgatc accggtctgg ttgtgggcct cgccatgttt tttgaagtgg
     5821 gttttgtcct gctgttgccg ttggtattta ccatcgtagc atcatcagga ttacccctgt
     5881 tgtatgttgg cgtaccaatg gtagcagcgc tctctgtaac ccactgtttt ctgccgccac
     5941 atccagggcc tactgccatc gcgactatct ttgaggctaa tctcggaacg actttactgt
     6001 atggatttat cattaccatt ccgacagtta ttgtcgcagg accgctgttt tctaaactgc
     6061 taactcgctt tgagaaagca ccaccggaag gcttatttaa tcctcatctg tttagcgaag
     6121 aggagatgcc ctccttctgg aacagtattt tcgctgccgt gatcccggtc atcctgatgg
     6181 ctatcgccgc cgtttgtgaa attacgttac cgaaaactaa caccgtgcgc ctcttctttg
     6241 aatttgtcgg taaccctgcc gttgcgctgt ttattgccat tgttattgcg attttcacac
     6301 tgggccgacg taatggacgc accatcgagc aaatcatgga tatcattggg gattctatag
     6361 gcgctatcgc gatgattgtg tttattatcg ctggcggcgg cgcgtttaag caggtattag
     6421 tagatagcgg tgtcgggcac tatatttcac acttaatgac cggaactaca ctttcgccgt
     6481 tattgatgtg ctggactgtt gcggcgctgt tgcgtatcgc tctgggctct gccaccgtcg
     6541 cggccattac caccgcgggt gtggtgttgc cgattatcaa cgttacccat gccgatcccg
     6601 ctttaatggt actggcaacc ggtgcgggca gcgtgatcgc gtcacacgta aacgaccctg
     6661 gcttctggct atttaaaggg tattttaatc tgacggttgg tgaaacgttg cgtacctgga
     6721 cggtgatgga aacccttatt tctattatgg gtttgctggg cgtgttagcc attaacgccg
     6781 tattgcactg aaagaataaa atattgtgat gtagtttcgg gggaagattg aagattcccc
     6841 cgacgttggg agaagttatg cgcaatcaca gaatttcttt acaggatatc gctacgctgg
     6901 ctggcgtaac aaaaatgacc gtgagtcgtt atatccgctc gccgaaaaag gtggcaaagg
     6961 aaacaggcga gcgcatcgcc aaaattatgg aagagatcaa ttatatccct aatcgcgctc
     7021 ccggcatgtt gttgaatgca caaagttata ccctcggtat cttgattccg tcatttcaaa
     7081 atcaactctt cgctgacatt ctcgcgggaa tagaatccgt cacctctgag cataattatc
     7141 agacccttat tgctaattac aattacgatc gcgattccga agaagagtcg gtgattaacc
     7201 tgctctctta taacattgac gggattatac tttcggaaaa atatcacacc atcaggacgg
     7261 tgaaatttct gcgttcagcc accattccgg tcgtggaatt gatggatgta cagggagaac
     7321 ggctggatat ggaggtcggt tttgataatc ggcaggccgc ttttgacatg gtgtgtacca
     7381 tgctggagaa gcgagtcagg cacaaaattc tgtatctcgg ttccaaagat gacacccgcg
     7441 atgaacagcg ttatcagggg tattgcgatg cgatgatgct gcataatctt tccccattac
     7501 gcatgaatcc acgcgccatc tcatccattc atttaggaat gcagttaatg cgtgatgccc
     7561 ttagcgcaaa cccggattta gacggtgtat tttgcaccaa tgacgatatt gcgatgggtg
     7621 cgcttctgct gtgccgcgag cgaaacctcg ccgtgccaga acaaatttcc attgcgggtt
     7681 ttcatgggct ggagattggc aggcaaatga tcccaagcct cgccagcgta attactcccc
     7741 gttttgatat tggtcggatg gccgcccaaa tgttgctgag caaaattaaa aataacgatc
     7801 acaaccacaa cactgttgac ctcgggtatc agatttatca cggcaacacg ctttaggtta
     7861 ttttctcttc attacacgta ttctctttgc cggacgttgc ctatactcca ggcaggaaac
     7921 tggaggatat ctcatgagtg aacccctgtt aattgcccgc acgccggaca cagaactgtt
     7981 tttactgccg ggaatggcta accgtcatgg gctgattacc ggcgcaacgg ggacgggtaa
     8041 aaccgttacg ctgcaaaaac tggcggagtc attgtcggaa atcggcgtgc cggtgtttat
     8101 ggctgatgtg aaaggcgatc tgaccggcgt cgcgcaggca ggaacggtgt cggaaaaact
     8161 gctcgcaagg cttaaaaata tcggcgtcaa tgactggcaa ccgcatgcca atccggtggt
     8221 ggtgtgggat atctttggcg agaaaggcca tccggtgcgg gcgacggttt cggatctggg
     8281 gccgctgttg ctggcacgac tgttgaatct caacgatgtg caatctggcg tgctgaatat
     8341 catcttccgc attgctgacg atcaggggct gttactgctc gactttaaag atctgcgggc
     8401 aattacccag tacatcggcg ataacgccaa atccttccag aatcagtacg gtaatatcag
     8461 tagcgcatcg gttggtgcca tccagcgcgg gctgttgtcg ctggaacagc aaggcgcagc
     8521 acacttcttt ggtgagccga tgctggatat caaagactgg atgcgcaccg atgccaacgg
     8581 taaaggcgtt atcaatatcc tcagcgccga gaaactttat cagatgccga aactgtacgc
     8641 cgccagcctg ctgtggatgc tttcagagtt gtatgaacaa ttgccggaag cgggcgatct
     8701 ggagaaacca aaactggtgt ttttcttcga cgaggcacat ctgctgttta acgatgcccc
     8761 gcaggtactg ctggataaga ttgagcaggt gataaggctt attcgctcaa aaggcgtggg
     8821 cgtctggttc gtttcgcaaa acccgtctga tattccggat aatgtgctcg ggcagctcgg
     8881 taatcgcgtt caacatgctt tgcgggcttt tacgccaaaa gatcagaaag cagtgaaggc
     8941 agcggcgcaa accatgcggg ccaatccggc gtttgatacc gaaaaggcga ttcaggaact
     9001 gggcaccggc gaggcgttga tctcttttct ggatgcgaaa ggaagccctt ctgtggtgga
     9061 gcgtgcgatg gtgatcgcgc cttgttcgcg gatggggccg gtgacggaag atgagcgtaa
     9121 tggcttgatt aatcactctc cggtgtatgg caaatatgag gatgaggtgg accgggaatc
     9181 cgcctatgag atgttgcaaa aaggctttca ggccagtacc gagcagcaaa ataatcctcc
     9241 cgcgaaaggg aaagaggtag cggtggatga cggcattctt ggtggattga aggatatttt
     9301 gttcggcact accggaccac gcggcgggaa gaaagatggt gtggtgcaaa caatggccaa
     9361 aagcgccgct cgccaagtga cgaatcagat tgtacgcggg atgttgggga gtttgctggg
     9421 ggggagaaga aggtaagtca ggcgagattt gtctttgcct gatgcgacgc tggcgcgtct
     9481 tatcatgccc accccactgc aatatattga attttaatta tttttcagga atgaacgaag
     9541 cacactttgc taacaataat aaagggagct ttcgctccct ttattcgttc attcggttac
     9601 gattttctca ttaacagcca caggctgatt aagaagaagc tggcgcttgg caacagtgcg
     9661 ccgatgatcg gcgggatgcc ataaaccaac gtcagcgggc cgaagatctg gtccagtacg
     9721 tagaagacaa aaccgaaact gataccggtg accacacgca cgcccatcgg tacgctacgc
     9781 agtgggccaa agatgaacga cagcgccatc agcatcatca ccgccacaga tagcggctgg
     9841 aagattttgc tccacatgtt gagctgataa cgtccggcat cctgaccgct cgacttcaga
     9901 tacttcacat agttgtgcaa accgctgata gagagtgcat ccgggtccag cgccaccacg
     9961 cccagtttgt ccggcgtgag gttggttttc caggtgccgc tcaccgtctg cgaaccggta
    10021 atctgtttcg gattggtcag atcagattca tcaacctgcg acagacgcca gactttatgt
    10081 tccgggtcaa acttcgcagt agcggcatag cgtacggatt gcagacgacg attctcgtta
    10141 aaggcataaa tgctgatgcc acctaactct tcgtcacctt taacccgctc aatgtagacg
    10201 aagttgttgc catctttcgc ccataagcct tgctgggtag agagcaacga gccgccgtac
    10261 atcgcctgcg cacggtagtt acgcgccatc tgctcgccct gcggcgcgac ccattcgcca
    10321 atcgccatcg tcagcaagac cagcggaatg gcggttttca tcaccgacag cgccacctgc
    10381 atacgggtaa aaccagaagc ctgcatcacc accagttcgc tgcgctgcgc cagcatccca
    10441 agaccaagca acgccccaag cagagccgcc atcgggaaga agatctgcac atctttcggc
    10501 acgctcagca aggtatacat tcctgcgcct aacgcgtcgt aactcccctg cccggctttt
    10561 ttcagctgat cgacaaactt gataatgccc gacagcgaca ccagcatgaa cagtgtcatc
    10621 atgatggtgg tgaaaatagt tttaccgata tagcggtcaa gtacgccaaa aggttgcatc
    10681 acaccgctcc tttacgcgaa aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa
    10741 ggttgagaac aatcgctaaa gccagataaa tcaggttaac ggtccacatc cacagcgtcg
    10801 ggtccagctt acctttaccg ccgttcgatt tcagggaggt ctggatcagg aagaaaagta
    10861 gatacagcag catggctggc agcatcgaca gtacgcgtcc ctgacgtggg ttaaccacgc
    10921 tcagcggtac gaccataagt gccatcataa acacggtgaa taccaacgtg atacgccagt
    10981 tcagttctgc gcgagcacga tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt
    11041 cggtatcgtt cgggtcgagc gccaccgcct ggtgaccaat gatcgcctga taatcctgga
    11101 aatccgtaat gcggaaatca cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga
    11161 gagtgacgac ctgggagccg tcgcgcagct gggttaaatg tccggaatcg gccaccacca
    11221 cagaaggacg tgcattacct tttggtcgaa tttgcgcgag gaacacatct ttgaaatcgc
    11281 tgccgtcaac gctttcgatg aacagcaccg aactgccatt agtcgcttgc tggaattgcc
    11341 cttgcgccag cgccgccatg ccagggttcg ctttcgcttc tgctaacact tcatcctgat
    11401 gacgcgatga ccacggtccc gcccacatca cgttaaccgc cgcgacgatt gccgtgaata
    11461 ccgcaaggat cattgccgct ttcaccagaa ccgctttgct caggccgcag gcatgcatta
    11521 ccgtaatttc actttcggta tacagtttgc ccagcgtcat cagcagcccg aggaacaggc
    11581 ttaatggcag gataagctgc gccatttccg gcacgcccaa cccgagaagg gagagcacca
    11641 gattcgccgg aatatcgccg tcaaccgctg cgccgaggat cctcactaac ttttgacaga
    11701 agaagatcaa aagcaagatg aagagtatcg ccagctggct tttgagcgtc tcccgcacca
    11761 gatatcttat gattatcact ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta
    11821 gcttgtttca tggcttaaac gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat
    11881 aattatactc aacggattca cctctcagat tttgttctga cgtgccaatg ccgtaataac
    11941 gttaagatta acacgaagtc atcgcaacag cggacatgag ttacgaaagc ttgcaattct
    12001 atctgtagcc accgccgttg tctttaagat tcaggagcgt agtgcatgga gtttagtgta
    12061 aaaagcggta gcccggagaa acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca
    12121 cgtcgccttt ctccgattgc agaacagctc gataaaatca gcgatgggta catcagcgcc
    12181 ctgctacgtc ggggcgaact ggaaggaaaa ccggggcaga cattgttgct gcaccatgtt
    12241 ccgaatgtac tttccgagcg aattctcctt attggttgcg gcaaagaacg tgagctggat
    12301 gagcgtcagt acaagcaggt tattcagaaa accattaata cgctgaatga tactggctca
    12361 atggaagcgg tctgctttct gactgaactg cacgttaaag gccgtaataa ctactggaaa
    12421 gtgcgtcagg ctgtcgagac ggcaaaagag acgctctaca gtttcgatca gctgaaaacg
    12481 aacaagagcg aaccgcgtcg cccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt
    12541 gaactgacca gcggtgagcg cgcgatccag cacggtctgg cgattgccgc cgggattaaa
    12601 gcagcgaaag atctcggcaa tatgccgccg aatatctgta acgccgctta cctcgcttca
    12661 caagcgcgcc agctggctga cagctacagc aagaatgtta tcacccgcgt tatcggcgaa
    12721 cagcagatga aagagctggg gatgcattcc tatctggcgg tcggtcaggg ttcgcaaaac
    12781 gaatcgctga tgtcggtgat tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc
    12841 gtgctggtgg gtaaaggttt aaccttcgac tccggcggta tctcgatcaa gccttcagaa
    12901 ggcatggatg agatgaagta cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg
    12961 atggtcgcgg agctacaact gccgattaac gttatcggcg tgttggcagg ctgcgaaaac
    13021 atgcctggcg ggcgtgccta tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc
    13081 gttgaagtgc tgaataccga cgctgaaggc cgcctggtac tgtgcgacgt gttaacttac
    13141 gttgagcgtt ttgagccgga agcggtgatt gacgtggcga cgctgaccgg tgcctgcgtg
    13201 atcgcgctgg gtcatcacat taccggtctg atggcgaacc ataatccgct ggcccatgaa
    13261 ctgattgccg cgtctgaaca atccggtgac cgcgcatggc gcttaccgct gggtgacgag
    13321 tatcaggaac aactggagtc caattttgcc gatatggcga acattggcgg tcgtcctggt
    13381 ggggcgatta ccgcaggttg cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac
    13441 ctggatatcg ccggtaccgc ctggcgttct ggtaaagcaa aaggcgccac cggtcgtccg
    13501 gtagcgttgc tggcacagtt cctgttaaac cgcgctgggt ttaacggcga agagtaattg
    13561 cgtcaggcaa ggctgttatt gccggatgcg gcgtgaacgc cttatccgac ctacacagca
    13621 ctgaactcgt aggcctgata agacacaaca gcgtcgcatc aggcaatgct gttattgtcg
    13681 gatgcggcat gaacgcctta tccgacctac acagcaccga actcgtaggc ctgataagac
    13741 gcggcagcgt cgcatcaggc aatgctgtta ttgtcggatg cggcgtgagc gccttatccg
    13801 acctacacac agcactgaac tcgtaggcct gataagacac aacagcgtcg catcaggcgc
    13861 tgcggtgtat acctgatgcg tatttaaatc caccacaaga agccccatat atgaaaaacg
    13921 caacgttcta ccttctggac aatgacacca ccatcgatgg cttaagcgcc gttgagcagc
    13981 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg
    14041 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
    14101 ttgttccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
    14161 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
    14221 cagattttgc caccgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
    14281 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
    14341 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
    14401 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
    14461 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
    14521 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctgtggca
    14581 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
    14641 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
    14701 atggaaagcg gaatccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactccgt
    14761 ggactgggag cgtgaacgct tcaccatgga cgaaggcctg tccaatgcgg tgaaagaagt
    14821 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aagcgcctgg taaactggga
    14881 cccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
    14941 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
    15001 tctggtggtt gcgactaccc gtccggaaac cctgctgggc gatactggcg tagccgttaa
    15061 cccggaagat ccgcgttaca aagatctgat tggcaaatat gtcattctgc cgctggttaa
    15121 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
    15181 gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc
    15241 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agtgcccagg tgttcgatac
    15301 caaaggtaac gaatctgacg tttattccag cgagatcccc gcagaattcc agaaactgga
    15361 gcgttttgct gcacgtaaag cggtcgttgc tgcggttgac gcactcggcc tgctggaaga
    15421 aattaaacca catgacctga ccgttcctta tggcgaccgt ggtggcgtgg tcatcgaacc
    15481 gatgctgacc gaccagtggt acgtgcgtgc cgatgtcctg gcgaaaccgg cagttgaagc
    15541 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    15601 gatgcgcgat attcaggact ggtgtatctc tcgtcagttg tggtggggtc accgtatccc
    15661 ggcatggtat gacgaagcgg gtaacgttta tgttggccgc aacgaagacg aagtgcgtaa
    15721 agaaaataac ctcggtgctg atgttgtcct gcgtcaggac gaagacgttc tcgacacctg
    15781 gttctcttct gcgctgtgga ccttctctac ccttggctgg ccggaaaaca ccgacgccct
    15841 gcgtcagttc cacccaacca gcgtgatggt atctggtttc gacatcatct tcttctggat
    15901 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    15961 gccgttccac actgtttaca tgaccggtct gattcgtgac gacgaaggcc agaagatgtc
    16021 caaatccaag ggtaacgtta tcgacccact ggatatggtt gacggtattt cgctgccaga
    16081 actgctggaa aaacgcaccg gcaatatgat gcagccacag ctggcggaca aaatccgtaa
    16141 gcgcaccgag aagcagttcc cgaacggtat tgaaccgcac ggtactgacg cgctgcgctt
    16201 caccctggcg gcactggcgt ctaccggtcg tgacatcaac tgggatatga agcgcctgga
    16261 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    16321 agaaggtcag gattgcggct tcaacggcgg cgaaatgacg ctgtcgctgg cggaccgctg
    16381 gattctggcg gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    16441 cttcgatatc gccgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    16501 tctcgagctg accaagccgg taatgaacgg tggcaccgaa gcagaactgc gcggtactcg
    16561 ccatacgctg gtgactgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    16621 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    16681 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    16741 caccgagtgg ctgaagcagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaacat
    16801 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    16861 cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct
    16921 gcctgccgat gacaaaggtc cggtttccgt tacgaagatt atcgacggcg cagagctgct
    16981 gatcccgatg gctggcctca tcaacaaaga agatgagctg gagcgtctgg cgaaagaagt
    17041 ggcgaagatt gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    17101 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga
    17161 agcgaaagcg aagctgattg aacagcaggc tgttatcgcc gcgctgtaat caacaccgtg
    17221 atgaaaacga aggccggagc atgctccggc cttttttatc tcttacagaa atggtaaaga
    17281 aggcataatt ccccgtgaaa tactggctaa gaagccttta tctggttgtt cttctttatt
    17341 gattaaagga aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat
    17401 catccatatt ttcagcagtg gataagccag accgcccgta atcccggata tcaccaccaa
    17461 cgcacacatg cgataaagca taccgtggta tgttaaagtt gagcgaaaac gaatacgccc
    17521 atcattcagt gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt
    17581 gctaacagca atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc
    17641 cataaattgc tgtaaattct caatatcatc atttgcatac acagaactat catacacatt
    17701 taatatttga tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa
    17761 tgcgagaatg gcatatttta tacaatacgt agtatctaat ttcaccttga aacgatgtat
    17821 accgaaactg gtattgctcc agagaaaact cattaccaga ctatataaag taccattaaa
    17881 aataccaatg gaaacaatac caactattgc catcagaacc acagatataa taacactact
    17941 tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc
    18001 cattaaaatc ggcaagaaaa aggttaccca ccagaaccct ttcaaagagc aattaaaact
    18061 aaatcttacg ccgttgagac tagtcatcaa ggcctgataa cgcagtcctt ttgctgccat
    18121 aaatataagc aaaaccaaca gtgacaaagt caaaacacag ccaacaagcg gcatatctgc
    18181 tgacactgtc attaagattg cgaaatagaa aaaaacaaaa acaagacaac taacaaaaac
    18241 attcccaccg gtaatcccat aagaaaatcg ttgtccgtta acttccatat tagcatagag
    18301 ataacgctta catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa
    18361 caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaacga
    18421 atgggacgga acatccattt cattaataac ttgagccata aaaattccct gaattaaaaa
    18481 atccat
//
LOCUS       DAPHJE010000091        18104 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPHJE010000091 DAPHJE010000000
VERSION     DAPHJE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.091"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(244..1836)
                     /gene="lsrK"
                     /locus_tag="RH222_003191"
     CDS             complement(244..1836)
                     /gene="lsrK"
                     /locus_tag="RH222_003191"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV3798172.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1915..2868)
                     /gene="lsrR"
                     /locus_tag="RH222_003192"
     CDS             complement(1915..2868)
                     /gene="lsrR"
                     /locus_tag="RH222_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV3798173.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3117..4652
                     /gene="lsrA"
                     /locus_tag="RH222_003193"
     CDS             3117..4652
                     /gene="lsrA"
                     /locus_tag="RH222_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV3798174.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4646..5674
                     /gene="lsrC"
                     /locus_tag="RH222_003194"
     CDS             4646..5674
                     /gene="lsrC"
                     /locus_tag="RH222_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV3798175.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5674..6666
                     /gene="lsrD"
                     /locus_tag="RH222_003195"
     CDS             5674..6666
                     /gene="lsrD"
                     /locus_tag="RH222_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV3798176.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYIKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6678..7700
                     /gene="lsrB"
                     /locus_tag="RH222_003196"
     CDS             6678..7700
                     /gene="lsrB"
                     /locus_tag="RH222_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV3798177.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7727..8602
                     /gene="lsrF"
                     /locus_tag="RH222_003197"
     CDS             7727..8602
                     /gene="lsrF"
                     /locus_tag="RH222_003197"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV3798178.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8626..8916
                     /gene="lsrG"
                     /locus_tag="RH222_003198"
     CDS             8626..8916
                     /gene="lsrG"
                     /locus_tag="RH222_003198"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV3798179.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8973..9731
                     /gene="tam"
                     /locus_tag="RH222_003199"
     CDS             8973..9731
                     /gene="tam"
                     /locus_tag="RH222_003199"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV3798180.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9735..10649)
                     /locus_tag="RH222_003200"
     CDS             complement(9735..10649)
                     /locus_tag="RH222_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HDV3798181.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10856..12307)
                     /gene="uxaB"
                     /locus_tag="RH222_003201"
     CDS             complement(10856..12307)
                     /gene="uxaB"
                     /locus_tag="RH222_003201"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV3798182.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12534..13952)
                     /locus_tag="RH222_003202"
     CDS             complement(12534..13952)
                     /locus_tag="RH222_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3798183.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRMLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14091..14450)
                     /locus_tag="RH222_003203"
     CDS             complement(14091..14450)
                     /locus_tag="RH222_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV3798184.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14450..15376)
                     /gene="glsB"
                     /locus_tag="RH222_003204"
     CDS             complement(14450..15376)
                     /gene="glsB"
                     /locus_tag="RH222_003204"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV3798185.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKT
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15440..16828)
                     /gene="sad"
                     /locus_tag="RH222_003205"
     CDS             complement(15440..16828)
                     /gene="sad"
                     /locus_tag="RH222_003205"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV3798186.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16929..17810
                     /locus_tag="RH222_003206"
     CDS             16929..17810
                     /locus_tag="RH222_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3798187.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17888..>18104
                     /gene="yneK"
                     /locus_tag="RH222_003207"
     CDS             17888..>18104
                     /gene="yneK"
                     /locus_tag="RH222_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV3798188.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRT"
BASE COUNT         4558 a         4625 c         4501 g         4420 t
ORIGIN      
        1 aaaatcgaag tttattaaac cccttttgca tccattagtg tctttaggaa tattcgctat
       61 aaaataaggg ttgtgcttaa tgctttaaga aaaatagcaa ttttcccttg aatatagtac
      121 tggtgatgga acgatgaatc tgcaagctgg cttttaacaa gccagctcta aaagaaggga
      181 aataagaata actatactca aaaactaaca gccacggtca tcatgatgtg gctgtcaatg
      241 aaactataac ccaggcgctt tccataacga cgtcgtcagt ccatgatcaa ccagccccag
      301 ctgatcctga taaactgcct gccacttatc gcgtgaatcc tgataaagtt catgcttttc
      361 cgggtctggt gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt ctgccattga
      421 tgaaaaaatt ccggcaccga cgccagctgc aatggcacat cctaatgcag tggcttcttt
      481 gaccaccgga atattgacgg gtaatcccga gacatcagcg agaatttgac tccataattt
      541 cccttttgaa cctccgcctg caaagactaa cgatgaagga tgaatattcg agaaatcagc
      601 aatttgctgc aagttacacg ctgatacaat cgccgcattt tcttccagcg cacggaacaa
      661 tgtcgctttg ttacatttat ccgggtcaat ggacaagtta ataaaggaag gcgcagcgtg
      721 ataccaggtt ttaaagcgca ttctgtcgga gaagatcggc attacgcccc acgacccagg
      781 cggcacccga ctggccatct cttccagcag cgtataggtg tcgatgccta aacgttcggc
      841 aatcagtttt tcttcggcac agaaagcatc gcggaaccag cgcatggtga gtccggtaaa
      901 aaagcttata gattcagctt gtaccatgcc aggaataaca tgaggattaa cgcgcacgtt
      961 catttctggg tctgtcaccg gcgcggctaa atttacaact tgctgccaga atgtgccgcc
     1021 aagaaccgcg gtttgtgccg gacgcacaac gcctaaccca aggcaaccaa gctgcacgtc
     1081 gcctcctcca acgaccaccg gagtgcccgc cttcagaccg cagagttccg ccgcttgtga
     1141 acttaccacg cccagcaatg tgccggtttc tttgacagga gaaagaatat cggcacgtag
     1201 gccagccata tccagcaatg caggtttcca gtcacgggtg gttagatcaa gaagtcccgt
     1261 ggtgccagcg ttagagggat ccaccgccag ttcgccgctg agcatatagg ccagccagtc
     1321 gctgatcatg gtgatggttg atgcctgacg gtaaatatcg gaacgatggt gcgccagcca
     1381 aagtaatctg gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat aaacttcgtt
     1441 ttcaaaggta ttgttgtgca gttctttaag ttcgctaact tcgcgtgccg ctctggcatc
     1501 cacattggcg caggcccaga tcggggctcc ttcattatta tataaaacaa tgccttcacg
     1561 catcgaacat gccgaaacgg cagcgatata ctccggggct atgccggcgt tgtgcagcgc
     1621 ctggcgcata cactcacacg ccagttgcca gtttttgttg agatcaaatt ccatagaacc
     1681 aggaacgtcc ggtactgcca ggtgccgcca ctccgcctgt cccactgcta tttgattgcc
     1741 ttccaggtcg aatatcacag cccgaatact tccggtacct gcatccagcg ccatcaggta
     1801 atactttgat tctgaagggg taaagagtcg agccattaga ttatcctcgg cttatgcgtt
     1861 ctgtctgttc ctctatacgt tctccatcat tcccggtaat aaggtcatgc aaatttaact
     1921 acgtaaaatc gccgctgctg tgtcctgatc ggtaaccagt gcgttgatat aaccgccttt
     1981 cattgcagcg gcaattgctt cggctttatt ttctccccct gccacgccaa cccggacggg
     2041 tatggtcttc agcgagctta aaggtaagcc aatcagttcg ttatgtattt taatatccgt
     2101 gacaacgtca ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg ccccttttcg
     2161 accaatcatt aactgttcgc cctggctgat ataaccggag cgaatgatcg tcgcgtcgtc
     2221 ctgttgactg acggccccaa tgccgacaat cgccacatcg gctgcttgcg cggctaacag
     2281 aacatctttg acgcaatttt cattttttag cgtacgggca atgtcagcgg aggatgcccg
     2341 caacggagcc ggaataatat tcacactgca cgccgcgtta agctgcccga tacccgtcat
     2401 ataagaaccg acgccaccgg agagcgtgac caggcgaatt tgctgtgacg aaataaaacc
     2461 acttaagcgt tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca gcatctgttg
     2521 tggttgaagt aaactcatca acatatgcgc cgcgcctatc cccagtcgcc cgccgacatc
     2581 agcatccgca agcccaggga tcacccggac atgttgcagc gaaaactgac gacgtaattg
     2641 agtttcatat tccagacagc cttcaaagcg agaattaatc tgtacgcgaa taatgccgga
     2701 ctgatgccct ttctccagca agcgcgacac tttcaaacgg gtcaggccga gacgatcgct
     2761 gatctcgctc tgggtcagcc cgtcgtgata gtaaaaccac gcgatccgcg cgacctgttc
     2821 ttcttcacac attccctgtt ctgaaattgc cgaatcgttg attgtcataa ttcattcttc
     2881 actttgaaca tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt tatatctgtg
     2941 atggcagcca cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa atcaaaatag
     3001 cataaattgt gatctattcg tcggaaatat gtgcaatgtc cacctaaggt tatgaacaaa
     3061 ttaaaagcag aaatacattt gttcaaaact cacctgcaaa actgaacggg ggaaatatgc
     3121 aaacgagtga tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat aaacagtatt
     3181 caggggtcaa tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag gtccacgccc
     3241 tgctcggcgg caatggtgcc ggtaaatcga cgttaatgaa gattattgcc ggtattaccc
     3301 ctgctgatag cggtacgctg gagattgggg gcaacaacta cgccagatta acgccagttc
     3361 atgctcatca gctgggcatt tatctcgttc cccaggaacc gctgcttttc ccaagcctgt
     3421 cgataaaaga aaacatcctg tttgggatgg caaaaaaaca gctctccatg caaaaaatga
     3481 agaacttgct ggcggcgctg ggctgccagt ttgatctgca tagtctggca ggatcgctgg
     3541 atgtcgccga tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac tcgcggattc
     3601 tgatcctcga tgaacctacc gcctcgctta cccctgcgga aaccgaacgc ttgtttagtc
     3661 gcttgcaaga gctgcttgct actggcgtgg gtattgtttt tatctcgcat aagctgccgg
     3721 aaattcgcca gattgccgat cgaattagcg tgatgcgcga cggaaccatc gccttaagcg
     3781 gcaaaaccag cgaactgtct accgacgaca ttattcaggc catcacccca gcggtacggg
     3841 aaaaatcgct ctctgccagc caaaaattat ggctggagtt acctggtaac cgcccacaac
     3901 atgctgccgg aacgccggtg ctgacactgg aaaatctgac cggcgaaggt ttcaggaatg
     3961 tcagcctgac gctcaatgcc ggagaaattc tgggcctggc tgggctggtg ggagccggac
     4021 gcacagaact ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga cgcattatgc
     4081 tgaatggtaa agagatcaat aaattatcca ctggagaacg tttactgcgc ggtctggttt
     4141 atctgccgga agatcgccag tcatccggac tgaatctcga tgcttcgctg gcctggaacg
     4201 tctgcgccct tactcataac cttcgtggat tctgggcgaa aaccgcgaaa gataatgcca
     4261 ccctggaacg ttatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa caagctgcac
     4321 ggacattatc cggtggcaac cagcaaaaaa tcctcattgc caaatgcctg gaagcttcgc
     4381 cgcaagtatt gattgtcgat gagccgacgc gcggcgtgga tgtctcggca cgtaatgata
     4441 tctaccagct gttgcgcagc atcgccgcac agaatgtggc tgtgctgctt atctcctctg
     4501 atctggaaga gatcgaactg atgacagatc gtgtgtatgt gatgcatcag ggcgaaatta
     4561 cccactctgc actgaccggg cgcgatatta atgtcgagac tattatgcgc gttgccttcg
     4621 gcgatagtca gcgtcaggag gcatcatgct gaagtttatt cagaacaacc gtgaaatcac
     4681 ggcactgctg gcggtggtgc tgctgtttgc attacccggt tttctcgacc gccagtattt
     4741 aagtgtgcaa acgctgacca tggtttatag cagcgcgcaa atcctgatcc tgctggcaat
     4801 gggcgcgacg ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt cgattaccgg
     4861 aatgtgcgca gtgctgttgg ggatgttact gaacgcgggg tattcactac ctgttgcttg
     4921 tgtcgcgact ttactgcttg gtttgctcgc gggatttttc aacggtgtcc tggtcgcgtg
     4981 gctaaaaatc cctgccattg ttgccaccct tggcacgtta gggttgtaca gaggcatcat
     5041 gttgctgtgg actggcggta aatggattga agggttaccc gccgaactga aacagctctc
     5101 cgcaccgctg ctgtttggcg tttccgcgat tggttggttg acgataattc tggtggcatt
     5161 tatggcctgg ctgctggcaa agacggcgtt tggacgcagt ttttatgcca cgggcgataa
     5221 tttacagggc gctcgtcaac tgggtgttcg tacggaagcc attcgcattg tggcattttc
     5281 gttgaacggc tgcatggcgg cactggcggg aattgtgttt gcttcgcaga ttggttttat
     5341 ccccaaccag accggtaccg ggctggagat gaaagcaatt gcagcctgcg tgctgggcgg
     5401 cattagtttg ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg catggttcct
     5461 gacgcagatc gatagcgtac tggtgctgtt gcgcattccg gcatggtgga acgattttat
     5521 cgcgggcctc gtgctgctgg cggtgctggt gtttgatgga cgcctgcgtt gtgcgctgga
     5581 acgtaatcta cggcggcaaa aatatgcccg ctttatgacg ccaccgccat ccgttaaacc
     5641 cgcttcgtca ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg ttgggaactg
     5701 gctcttgccg cactgctcgt tattgagatt gtcgcatttg gtgcaattaa cccgcgaatg
     5761 ttagatctca atatgttgct gttcagcacc agtgacttta tctgcattgg cattgtcgcc
     5821 ctaccgctga cgatggtgat tgtcagtggc gggatcgata tttcgtttgg ttcgaccatc
     5881 ggcctctgcg ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat gccgctggcg
     5941 attctcctga ccttactgct cggcgcattg tgcgggctga tcaacgccgg attaattatc
     6001 tatatcaaag ttaacccgct ggtgattacg cttggcacgc tgtatctgtt tgccggaagc
     6061 gccctactgc tttccgggat ggccggagcg acggggtacg aaggtattgg tggattcccg
     6121 atggcgttta cagatttcgc taacctggat gtgctgggac tccccgttcc gctgattatc
     6181 ttcctgatat gtctcctcgt tttctggctc tggctgcata aaacccatgc cggacgtaat
     6241 gtgtttttga ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc agttaaccgc
     6301 accttatgtg cgctctatgc catgacgggg ctggcgtctg cggtcgccgc tgtgctgctg
     6361 gtgtcgtatt ttggttcagc acgttccgat ctcggtgcgt cgtttctgat gcccgccatc
     6421 accgccgtgg tgcttggcgg tgccaatatt tatggtggtt ccggttccat tatcggcacc
     6481 gccattgcgg ttttattagt gggatatttg caacaaggtt tgcaaatggc aggagtgcca
     6541 aatcaggtgt ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt aggtcgttcc
     6601 gttagcctgc atcgccagca aattaaagag tggctggcgc gtcgggccaa taacccattg
     6661 ccataaagga tatcttcatg acacttcatc gctttaagaa aatcgcctta cttagcgctc
     6721 ttggcattgc cgcaatctct atgaatgtgc aggccgcaga gcgtattgca tttattccca
     6781 aactggttgg cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa gcgggtaaag
     6841 agctgggcgt tgatgtgacc tacgacgggc cgacagaacc cagtgtttct ggtcaggtac
     6901 agttgattaa taacttcgtc aatcaaggtt ataacgccat tatcgtttct gcggtttcgc
     6961 ctgatggctt gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga gtgctgacct
     7021 gggactctga tactaaaccg gagtgccgct cttactacat taatcaggga acgcccgccc
     7081 agttgggagg tatgttggtg gatatggcgg cgcgtcaggt gaataaagac aaagccaaag
     7141 tcgcgttttt ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg aaagaagcga
     7201 aagcgaaaat cgccaaagag catccaggct gggaaattgt cactacgcag tttggctata
     7261 acgatgccac taaatcgtta caaacagcag aaggaatatt aaaagcgtat agcgatctcg
     7321 acgccattat cgcccccgat gccaacgccc tgcccgctgc cgcacaagcc gcagaaaacc
     7381 tgaaaaatga caaagtagcg attgtcggat tcagtacgcc aaatgtgatg cgcccgtatg
     7441 tagagcgcgg cacggtgaaa gaatttggcc tgtgggatgt ggttcagcaa ggcaaaattt
     7501 cagtgtatgt cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc gacaaactgg
     7561 atatccaggg agtaggtcag gtggaagtct cgccaaacag cgttcagggc tatgactacg
     7621 aagcggatgg taatggcatc gtactgttac cggagcgcgt gatattcaac aaagagaata
     7681 tcggcaaata cgatttctga tgtgcattac ttaaccggag taagttatgg cagatttaga
     7741 cgatattaaa gatggtaaag attttcgtac cgatcaaccg caacaaaata tcccttttac
     7801 cctgaaaggt tgcggtgcgc tggattgggg aatgcagtca cgcttatcgc ggatatttaa
     7861 tccgaaaacg ggtaaaaccg tgatgctggc ttttgaccat ggttattttc agggaccgac
     7921 taccggactt gaacgcattg atataaatat cgccccgctg tttgaacatg ccgatgtatt
     7981 aatgtgtacg cgcggcattt tgcgcagcgt agttcccccg gcgaccaata agccggtggt
     8041 actgcgggcg tcaggtgcga actctattct ggcggaatta agtaatgaag ccgtggcgtt
     8101 atcgatggat gatgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg
     8161 cagcgaatat gaacatcagt cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa
     8221 agtgggaatg ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg
     8281 ttatttctcg ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta
     8341 ttatgtcgaa aaaggttttg aacggattgt tgccggatgt ccggtaccca ttgttattgc
     8401 tggcggtaaa aaattaccgg agcgcgaggc gctggaaatg tgctggcagg ctatcgatca
     8461 gggcgcttct ggtgtggata tggggcgtaa tattttccag tctgaccatc cggtggcgat
     8521 gatgaaagcc gtacaggcgg tggttcacca taacgaaacg gctgatcggg catatgaact
     8581 ctatctgagt gaaaaacagt aactgcggat ttaaggagaa gaattatgca cgtcacactg
     8641 gttgaaatta acgttcatga agacaaggtt gacgagttta tcgaagtttt tcgccagaac
     8701 cacctgggct cggtacagga agaaggcaat ttgcgcttcg atgtcttaca ggacccggaa
     8761 gtgaattcgc gcttttatat ctacgaagcc tataaagatg aagacgcagt ggcattccat
     8821 aaaaccacgc cccactacaa aacctgtgtc gcgaaactgg aatctttaat gaccgggccg
     8881 cgtaaaaaac gtctgttcaa tgggttgatg ccgtgaggcg aatttatcaa ttttatctac
     8941 aattggggta acgcgctgac gggagtaaaa aaatgtctga ctggaacccc tctttatatc
     9001 tgcactttgc cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtgccgctgg
     9061 agaatgtcga atatgttgcc gatcttggct gtggcccagg taacagcacc gcccttctac
     9121 aacaacgttg gcctgctgcc aggataacgg gcatcgactc gtctccggcg atgattgctg
     9181 aagcgcgcag tgctttgcca gactgccagt tcgtggaagc agacatccgc aactggcaac
     9241 cggtacaggc actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact
     9301 acgaattgtt tcctcatctg gtttctttac ttaatccgca gggcgtgctg gcagtacaga
     9361 tgccagataa ctggctggag ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa
     9421 actacccgga tcgtgggcgt gagccgttgg ctggcgttca tgcttactac gatattttga
     9481 gcgaagccgg atgtgaggtc gatatctggc gaaccaccta ctatcaccag atgccgtcac
     9541 accaggcgat tatcgattgg gtgaccgcca ctggattacg tccgtggtta caggatctga
     9601 ccgagagcga acagcagctt tttcttaagc gctaccatca gatgctggaa gagcagtatc
     9661 cactgcaaga gaacggacag atactgctgg catttccgcg tctttttatt gttgcccgcc
     9721 gtacggagta aatatcacgt cagctggtaa tgacgatcgg gaagaatttt tgctggaatt
     9781 tcggcttcat cgttcatctg taacaggtca atttcaatag cgttgcagat ggcatccagc
     9841 ggtaaatcat tgttttcagt accgaacgga tcttccagtt cttccgccag acaatccagc
     9901 gaaataaaag tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg
     9961 accagcgcga acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg
    10021 taggcaaagg gaattggcgt ataggcaatg cgctcacagc ctgccagaac cgctgaaata
    10081 tcattaagcc gatcgttgag gctaataaac aggatatctg aaagctgtcc attgcggcgc
    10141 tgaaccgcca accattctcc cattattaac aagatacggt tagctggaga gttcgaagcc
    10201 agtacacgct gaagatcttc agtcttgaga taatgagcca gcacttccgc ctgtggctgt
    10261 ttgcgtaatg tcatgcgtaa acagtgggcg aaggcgattt gcagccgggc aaactccctt
    10321 acacttgccg aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg tgaggcaatc
    10381 atcaactgcc cccaaagttt tcgcgcttca acgtaacggg cgtacccggc attattacga
    10441 aaaccaagaa aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaatttg
    10501 atgcccagat gcgtgtacca gggcagcatg aatataacag cgatagaaaa gagaaaattg
    10561 agtagtaagc gcgaggatat cttggataat actgagccgt gccagacaaa aatacggcgc
    10621 agccagtgtt gttgtggacg aacaatcatg gttatcttca ggcgtggaaa agtcgcccta
    10681 ttaaacgtga ttacgatcac attctcaaga cgctacttac aaattaccta caagcttaca
    10741 acattaacag gcgctgcatg tggcacctgt taatgataaa ggcacatagg atgttgtaac
    10801 taatatagtg aaataagaaa cccggtcgaa accgggttca gaagtaacgg tgttattagc
    10861 acaacggacg tacagcttcg cgcatccctt tttcaagaat cgcatccagg tcattagcaa
    10921 cctgctcgac cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca
    10981 aaacaatcgc taccagttcc tgggtgccga taacacggtc acgatgctgg ctccagagtt
    11041 gctgataacg ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccgttacgct
    11101 caccgcgata gaaggcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc
    11161 cttttgcctt ctgccctgcc agcagctgcg gcaggatgcg ggtgcggaac ttggtcatac
    11221 cgttgagcgc gatagacagc agctgatgct taatgtacgg gttacggaaa cgcccggtga
    11281 ctgcactggc gaaagattcc aattcatcac gaggcaaatc cagtaccgga ataatttctt
    11341 cgtagatagc tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg
    11401 tatccagccc tgcctggaac gccaccggca ccagcgcggt gtgagcaccg ttgaggattg
    11461 ccactttgcg ctctttatac ggtttaatat cgtcaacaat cagcacgttg agtggatatt
    11521 tgtccaggcg cagctcagtc gctaaggatt tcggcccctg aatcacaaac aggtaaaagt
    11581 gttcagcggt gtcgaggaaa ccatcgtgat aacccagctc ttcttccagt ttagccactt
    11641 catcgcgcgg ataaccggta acgatacggt ccaccagcgt agaacagaag ctgttagcct
    11701 gatccagcca ttgaataaat gcttctggca gtgcccactc ttgcgcatag cgcagcacca
    11761 gttcacgcaa cgcgtcgcca ttgtagtcga tcaactcaca cgggatgatg atccaacctt
    11821 tatccagcgc accgttgaaa tggctaaaac gttcgaacag cagacgggtc agttttgccg
    11881 gataacttac cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg atacccgctt
    11941 cggtggtatt agagaaaaca aagcgcattt ccgggttgtg agccagtttc aggaattcat
    12001 cgtattcact gtagacgctg atttcacgat taaccgagcg aatcagacgc gcgtcgctga
    12061 ccgcttcccc cttctcattc aggccacgga taatggtggt atacagacca tcctgcgtgc
    12121 tcagtgacgg tgggaatgaa gtttcaatcg gacgaacaac gaccacacca gaattcagat
    12181 cggtgtgctc attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg aagttacctt
    12241 caccaaactg aatgatacgt tctggatact gtgcaccggg aaaatcgcga cgatttagtg
    12301 ttttcacaat gggttccctt ctgattagtc atacaacctg tttgaattgg tacgacaagt
    12361 tagcaaactt taatacgccg aacccctgtt ttgatcaact cctgatgatt aatgagcagt
    12421 tttatgagaa aagtgtggcg cggatcatgg tttaatcgag gaaatacgcc ttttcctgga
    12481 tcataaagtg gtagaacaca ttgcattcaa atcgcgcgga atgaataaag atgtcagaca
    12541 acttcctcac cgtaacgcat agtgctggta cggttgcgcc catctttctt cgagcgatac
    12601 agacacgtat cggcttcgac catcaattta ttaaaatcat cggtcagcat tcggtacgat
    12661 gcgcgaccac taccgacgcc aatacttacc gtgagataaa gcgttttttg ttgccaggtg
    12721 aatggttgca gttcaacgcc tttacggatt ttttccgcca ttagcagacc atcgacagga
    12781 ttcaccgacg gcactgcaac agcaaattct tcgccgccca tgcgcgccac cagcccctta
    12841 tcaccgacaa tcttctgaat atgccgggca aacacgctta acactttatc gccacattca
    12901 tgtccatagt tatcgttaat gcttttgaaa tagtcgatat caagcagcat gaccgtcaga
    12961 tgttgtgtct gtttcagcga tggacttttc agcgcctcat aaagaccgga ccgtgagtaa
    13021 acctgagtca gaaaatcaaa gtcggctcgc agcgcaactt gcttcattag cgaattgatc
    13081 gctgccacgc taaaagaaac cataattggg catatcgcca tcgtggcaat accgagacgt
    13141 gcggagaaca tttccggaat ggagaacggc gaaccgaccg agatatcaat caccgaattt
    13201 gccaccagta cgatttccac cgcaccggtg acaaaggtca acagacatgt tacctgcggc
    13261 gtatagcgca ctgcacacca gattaatgca ggcagcggga acgccagact ccccgctccg
    13321 ccaatgacta ccgaggcaat aaccgacaca ataagcgcga tggcgggcat catctgttct
    13381 gctttaaagc gcggcagcac tccaggaata gccaacgtca gcatacaagg cacgatcaac
    13441 acgcccgttg agaattgctc actgaaccag tccgcaagca aaggccagaa atccagacta
    13501 tcaatactga ccgaaccaat cgccccgaca atagcgcata ataatgcggc aatcagacag
    13561 taattaaata gccgtaaggc acttactggc tcatacttat ttttccccag acgcttatcc
    13621 cgcacgacca gtaaggcgac ggtaacaata aacatcatat tggagaaatt gataaccagt
    13681 gaaaccagtc cccattcggt ggttatggca tcataaacca gcatcgccac ataactgatc
    13741 gcatagtaat gcagtcgatt aagccagaca tagcgggcaa acacccccgc cattacgcca
    13801 ttcagcggcc agaaaagaga aagttcgtga accagccgta actctgcacc aataaaatag
    13861 aacagcgtgg ttagcgcaaa aatagcgatg ctattacgta gcagatgacc ttcctggaac
    13921 aaacggaaag ttgaaatggg gtgtacatgc attaattatc tgtccataca attgcgggtg
    13981 gcaattatct caacgtaata agtaaattct cataaaggat agcatgttcg ctaacagcgg
    14041 cggcaaataa aggtaccctg ggaatgctat tttatcgttt tccgcaattc tcatggctga
    14101 ttcatctgta caaccagcca gtgatagatt accgccacga tataacgttg ttgttcttcg
    14161 cttaacgaaa caccctgcgg aatgttatgc cacttagcca gacaaccgcg acagcaggtc
    14221 gcggtggcgt gctgagcgat aaacaccgga tgcccgcgca tgggggtttg cttaccgtca
    14281 ttagccggta atgccggagc aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg
    14341 actggcgcgc ctttctccag gcaatactga cgctctttca tgcccagacg aaagcgagag
    14401 cgaaattttg aacgggataa acgcgcgaag agtggatcga gagactgcat taataaaccg
    14461 aacgccctaa ctgtttcgtc aattgttcaa ggacggcaat acccgcaagc gagttacctg
    14521 catcatccag ttccggactc cagacagcga tggccatttc atgcggaaca atcgccacaa
    14581 taccgccacc aacgccagat ttcgccggta gccccacccg ccaggcaaac tcccccgcgt
    14641 tctggtacat accactggtc gccatcagcg cgttaatttg ccgcgcctgc attggcgtca
    14701 ccacaggttc atcaatatga atagttttcc cctgattagc cagaaagaca aacgtcctgg
    14761 ccagctctac acagctcatt ttcagagcgc agtaatgaaa gtagttttgc agaacggttg
    14821 tcacatcatg atggaaattg ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg
    14881 cggaatgttc aaattcggaa cgcgctacca ccgtatcgta tgaaatatca gacacaccgc
    14941 ttaagccgcg cacgacttcc agcatacgtt gccgtggtgc gcttaatcgc ccttgcaaca
    15001 tatcgcaaac caccagcgca ccagcattaa taaacggatt acgcggaata ccctgctcca
    15061 tttccagttg cactaaagaa ttgaacggtg atccagacgg atctttgccg acgcgttgcc
    15121 agatttcctc ttcggagtaa tgacgcatgg cgacaacgag actcagcact ttggaaatag
    15181 actgaatgga aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac
    15241 agatagcaat gcccaatcgg gaaccgtcta ctgtagccag cgccggaata taatccgcga
    15301 ctttaccctg accaatgagc ggccgcactt gccgcaagat gttttctaaa attgcattat
    15361 ccatggcgac tgccactttc tactcctgga ccgcaggtct gaaaagacct gcgagtatat
    15421 cagagctgaa tatgtcgcgt cagatccggt ctttccacac cgtctggata ttacagaatt
    15481 cgtgtaagcc gaaatgggaa agctcacgac caaagccact ctttttcacg ccaccaaagg
    15541 ccactcgcgc gtcgctggca caataaccat tgataaacac cccaccgcat tccagacgtg
    15601 ccgccatctg tctggcctgt gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact
    15661 cactatcatt agccagttcc agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa
    15721 cagggccaaa catttcttcc cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg
    15781 ttggcggata gtagttacct gccccagcca tcttttcccc gcccagtaac aaacgcgcac
    15841 cctgcgccag ggttttctcc acctgatgat gcagctcatc acgtaaatca aaacgagcca
    15901 ttggtccgag agcgttctct tcatcacggg gatcgcccat tttcaaggct gccgcagctg
    15961 ccacaaaacg ttcggtaaat gcagaagcaa ttccctcttc gataataaag cgttttgccg
    16021 ctgcacaaac ctgtcctgta ttctgataac gtccggctac cgccgcttta accgccagtt
    16081 ccagatcggc atcgttaagc acaataaatg gatccgaacc gcccagttcc agtacgcatt
    16141 ttttcagtgc cgctccagcc tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca
    16201 ccgtgacagc agcaatgcgc gaatctttaa tcatttgact gacaccgtcg ttgtcggcat
    16261 tcagccagcc atatacgcct tgcgggattc ccgcatcttt aaacacctgg gcaatgagct
    16321 gagcacagcc catcacattc ggcgcatgtt taagtaagta gccgttacct gcaagaatga
    16381 tgggaaccgc gccacgcatc acctgccata acggaaagtt ccacggcata atcgccagaa
    16441 tcgtccccaa cggtcgatac tcaataactg cctgctgatt ttccaccagc gtaggttccg
    16501 ccttcagcat tgccggacca tgttctgcat accagtcaca caaattcgcc gatttcgcca
    16561 cttcagcgcg cgcctgattg attggtttgc ccatttcacg ggtgatcatt tgcgccattt
    16621 cttcgctacg ggcgcgcaga gccttaccga taccacgcag tttttcagca cgataatcta
    16681 tatttgtctc gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat
    16741 cgttagcgcc agcccacggc agcacagaaa gttgttcacc cgtggcagga tttatcgaaa
    16801 ttgcatgagt tgccggagta atggtcatcg gggtatctcc tttatgagtc atggtatgaa
    16861 gatacgcaga tttactcttg ctttaaaatg aataatatta agccacttat tcacgaatcg
    16921 agaatattat ggatctgacc caactggaga tgttcaacgc ggttgccgaa gctggcagca
    16981 taacccaggc agcagcaata gtgcatcgcg tgccgtcgaa tctcactacc cgtctacgcc
    17041 agctggaaac agaactgggg gtagatctgt ttattcgcga gaatcagcgt ttacgtctct
    17101 ctcctgccgg gcataacttt ttacgctata gccaacaaat tctcgcgtta gtggatgaag
    17161 cgcggagcgt tgtcgctggc gatgagccgc aaggtttatt ttctcttggt tcgctggaaa
    17221 gcaccgcagc agtgcgcatt ccggccacgc tggcggaatt caaccatcgt tatcccaaaa
    17281 ttcagttttc actttccacc ggcccttccg gcactatgct ggaaggagtg ctggagggaa
    17341 aactgaatgc ggcatttatt gatgggccca ttaaccatac tgccattgac gggataccgg
    17401 tataccgcga ggagctgatg atcgtcacgc cacaaggtca tgcgccagta atccgcgcca
    17461 gtcaggttaa cggcagcaac atttatgcct tccgcgccaa ttgttcgtat cgtcgccact
    17521 tcgagagctg gtttcatgct gacggtgccg ctccgggaac tattcacgag atggagtcgt
    17581 atcacgggat gttggcctgt gtggtcgcag gagcaggcat tgcgcttatt ccacgctcta
    17641 tgctggaaag tatgccgggg catcaccagg ttgaagcgtg gccgttagct gagcaatggc
    17701 gttggttaac aacctggctg gtctggcgtc gtggtgcgaa aacgcgtccg cttgaggcat
    17761 ttattcaact actggatgtg cctgactcgg caaaacaggg ttaccaatga gctatttttg
    17821 atagttcttg cagctcaaat gccctatata gtgatgttcc gatgacttat gactatatgg
    17881 ggcaaatatg gttacgccag taagcatcag taattacata tctcttcctg atgattttcc
    17941 cgtacgtaat attgcacctc aggtaaaaga agttttaaag gattttattg atgcactcag
    18001 taccataata tgtgatgaag agtggcgcac gtctttaaac accaactcag ccacaaaaaa
    18061 gatatttaac aaccttgata acttatcata tattcagagg actt
//
LOCUS       DAPHJE010000092        17966 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPHJE010000092 DAPHJE010000000
VERSION     DAPHJE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.092"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..573
                     /locus_tag="RH222_003208"
     CDS             19..573
                     /locus_tag="RH222_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3798189.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /locus_tag="RH222_003209"
     CDS             complement(583..2010)
                     /locus_tag="RH222_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDV3798190.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYAQSWREAEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /locus_tag="RH222_003210"
     CDS             complement(2007..2714)
                     /locus_tag="RH222_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3798191.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2878..3855
                     /locus_tag="RH222_003211"
     CDS             2878..3855
                     /locus_tag="RH222_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDV3798192.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3925..4407)
                     /locus_tag="RH222_003212"
     CDS             complement(3925..4407)
                     /locus_tag="RH222_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV3798193.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4642..7224
                     /gene="leuS"
                     /locus_tag="RH222_003213"
     CDS             4642..7224
                     /gene="leuS"
                     /locus_tag="RH222_003213"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV3798194.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7239..7820
                     /gene="lptE"
                     /locus_tag="RH222_003214"
     CDS             7239..7820
                     /gene="lptE"
                     /locus_tag="RH222_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV3798195.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7820..8851
                     /gene="holA"
                     /locus_tag="RH222_003215"
     CDS             7820..8851
                     /gene="holA"
                     /locus_tag="RH222_003215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV3798196.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8853..9494
                     /gene="nadD"
                     /locus_tag="RH222_003216"
     CDS             8853..9494
                     /gene="nadD"
                     /locus_tag="RH222_003216"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV3798197.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9518..10129
                     /locus_tag="RH222_003217"
     CDS             9518..10129
                     /locus_tag="RH222_003217"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV3798198.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10389..10706
                     /gene="rsfS"
                     /locus_tag="RH222_003218"
                     /gene_synonym="rsfA"
     CDS             10389..10706
                     /gene="rsfS"
                     /locus_tag="RH222_003218"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV3798199.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10710..11177
                     /gene="rlmH"
                     /locus_tag="RH222_003219"
     CDS             10710..11177
                     /gene="rlmH"
                     /locus_tag="RH222_003219"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV3798200.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11208..13109
                     /gene="mrdA"
                     /locus_tag="RH222_003220"
     CDS             11208..13109
                     /gene="mrdA"
                     /locus_tag="RH222_003220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV3798201.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13112..14224
                     /gene="mrdB"
                     /locus_tag="RH222_003221"
                     /gene_synonym="rodA"
     CDS             13112..14224
                     /gene="mrdB"
                     /locus_tag="RH222_003221"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV3798202.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14235..15323
                     /gene="rlpA"
                     /locus_tag="RH222_003222"
     CDS             14235..15323
                     /gene="rlpA"
                     /locus_tag="RH222_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV3798203.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15463..16674
                     /gene="dacA"
                     /locus_tag="RH222_003223"
     CDS             15463..16674
                     /gene="dacA"
                     /locus_tag="RH222_003223"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV3798204.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16785..17048
                     /locus_tag="RH222_003224"
     CDS             16785..17048
                     /locus_tag="RH222_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV3798205.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17149..17790
                     /gene="lipB"
                     /locus_tag="RH222_003225"
     CDS             17149..17790
                     /gene="lipB"
                     /locus_tag="RH222_003225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV3798206.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4551 a         4502 c         4583 g         4330 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagatcaccg ggcagtcttc cattcaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaacg tcgcaatatc gccttggaga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcggaacagg aaaatccacg cgcgcaaagt aaactggggt ggatctacct caaaggtctg
      361 ggggtcaaac ccgacacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttaggtttg atctacagaa atggctcagg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccgggct tcccgcacat taacgtcgac tgtcacccga acagatggag
      601 tattttccct atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag
      661 acagcctggc ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt
      721 ttgcttctcg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga ttttctgcat caaggcggct cacaatcggc attaatgatt cttctgtagg
      841 ttggcaggcc tgcactaatt gtttggcaac tagatataat ggccataagg gatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc
     1081 ttcaatcatc atcaccagta atgggtaata atcgacccga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa gttctgccaa
     1201 caggctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataag gcgcatcttc
     1261 attatcaggt tcagcttccc gccatgactg ggcgtaatcc agaagttcgg ggattcccaa
     1321 gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggatgat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 gagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatatcgagg atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg
     2041 catgcagaat agtaagccaa tggttgccag gacaaggtag ggtaatacgc atcatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagctcgtag atgaatgcgc
     2281 gactggatcc atagactttc ttcttcgctc aaccagttat taagtacgcc cattcggctt
     2341 aaaaaaccca tccggacata atcccaggct ttgattcccc cttcgccaca gcattcggcg
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc aagatagtca
     2461 cgccattcac atggcgaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttat cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttgggtga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gactccatgt ccatataatt tatccttaaa tattcattaa atgaatatta gccgcattgc
     2761 gaggctcgtt tatttatact tttctgacgc gacataatac gcataaaata aggcttacgc
     2821 aaaaatggaa cacataaaaa tataatatgt tttatctgca accataagga aatgataatg
     2881 attttcacgt caacttgctg cgataattta tcaatagatg aaattatcga acgtgctgaa
     2941 aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt
     3001 gattctccgg acgacgaaaa agccttttac tggctgaagc tggccgccga gcaaggtcat
     3061 tgtgaagcac aatattcctt aggacaaaag tatactgagg ataaaagccg tcataaagat
     3121 aatgagcatg caatcttttg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc
     3181 aacgcccttg gctggatact ggatcgtgga gaagacccca attataaaga agcggttgtc
     3241 tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct tgggtggatg
     3301 tacagaaatg gcaacggagt cgcaaaagac tatgcgctgg catttttctg gtacaaacaa
     3361 gctgcattac aaggccatag tgacgcgcaa aacaatctgg ccgatcttta tgaagacgga
     3421 aaaggcgttg ctcaaaacga gacactcgcc gcattctggt atttgaaaag cgcacagcag
     3481 ggtaatcggc atgctcagtt tcaaattgca tgggattata acgctggcga aggggtggac
     3541 caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcaggg gagcgtcggc
     3601 gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag
     3661 gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac
     3721 ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac
     3781 ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact
     3841 gaagatttac tgtagagata aaaacaaggg ccggggaatt tcattcggcc catattctct
     3901 ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcatg
     3961 accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca
     4021 tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg
     4081 attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga tatctgccag
     4141 ctcctgccat aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag
     4201 gctctcttca tagctcatgg cgaactcttc caggtcacgt cttacagctc gcgtcagctc
     4261 atcgacctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac
     4321 cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ctcagtgacg cgaccagttc
     4381 acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa
     4441 taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt
     4501 atgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg
     4561 tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg
     4621 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     4681 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     4741 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     4801 cgtaactaca ccatcggtga cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc
     4861 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     4921 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     4981 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     5041 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taagaagact
     5101 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     5161 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     5221 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
     5281 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
     5341 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
     5401 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     5461 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
     5521 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta
     5581 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     5641 gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc
     5701 ctgaacatca aaccagttat cctggctgct gacggctctg agccagatct ttctcagcaa
     5761 gccctgactg aaaaaggcgt gctgttcaac tctggcgaat tcaacggtct tgaccatgaa
     5821 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     5881 aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc gccgattccg
     5941 atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct gccggtgatc
     6001 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg
     6061 gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
     6121 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
     6181 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     6241 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     6301 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     6361 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     6421 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     6481 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     6541 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     6601 atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc
     6661 tggaaactgg tttacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg
     6721 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc
     6781 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     6841 aacaaactgg cgaaagcacc aaccgatggc gagcaggatc gcgctctgat gcaggaagcg
     6901 ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt cacgctgtgg
     6961 caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     7021 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
     7081 atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     7141 ctggtagcaa aatatcttga tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa
     7201 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt
     7261 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gataccacgc
     7321 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
     7381 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     7441 cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat
     7501 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
     7561 tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt tcgttctttg
     7621 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
     7681 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     7741 gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct
     7801 ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     7861 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     7921 ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc acactttttc
     7981 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt
     8041 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa
     8101 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga tcgtccgcgg
     8161 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgcgcttg cgaatcgcag
     8221 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgcgcg
     8281 cgcaaaacag ctcaacttag aactggatga cgctgcaaat caggtgctct gctactgtta
     8341 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
     8401 cggcaaattg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
     8461 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca
     8521 gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac aacgtgaact
     8581 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     8641 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     8701 tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     8761 agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa
     8821 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     8881 gcacctttga tccggtgcac tatggtcatc ttaaacccgt ggaaacgctg gcgaatttga
     8941 ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     9001 aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     9061 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     9121 aagagtggcg gcaggaacaa gggccggacg tgccgctggc gtttattatt ggtcaggatt
     9181 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     9241 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     9301 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     9361 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     9421 acggtgaatc gtgtgaggat ttattgccgg aaccggtact gacttacatt aaccaacaag
     9481 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     9541 tggcgaaacg caagcgaatg tcgatggtct gtacagcggt catgcgccca cacccctgac
     9601 cgcacgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg tttcctttga
     9661 tctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
     9721 ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg gcgactggga
     9781 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     9841 tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     9901 ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt tagtcgtcag
     9961 ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg ccgaggctat
    10021 gtggcatttt cgcgttgacc aggggtgttg gagcaccatt gatatcaacc aaaaattcgc
    10081 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac ttttctgttt
    10141 ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat tctccgcagc
    10201 cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    10261 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    10321 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    10381 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    10441 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    10501 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    10561 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    10621 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    10681 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    10741 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    10801 tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg gacatcaagc
    10861 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    10921 ccctcgatat tccaggcaag ccctgggata caccgcagtt agccgctgag ctggaacgct
    10981 ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    11041 cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc ccccatccgc
    11101 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    11161 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    11221 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcat
    11281 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    11341 ttcgctttac cgactaccag actcgctcta atgaaaaccg cattaagctg gtgcctatcg
    11401 cgcccagccg cggcattatc tacgaccgta atggtatccc tctggccctc aaccgcacta
    11461 tctaccagat agaaatgatg ccagagaaag tcgataacgt gcagcaaacg ctggacgctt
    11521 tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    11581 gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta caagtagctc
    11641 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    11701 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    11761 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    11821 tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    11881 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    11941 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    12001 tgctggcggg tagccgcgca gctgtggttg tcaccgatcc gcgtacaggt ggggtgctgg
    12061 cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    12121 attattccgc cttgttgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
    12181 tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcattg agcgccgggg
    12241 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactgcca ggttcggaaa
    12301 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    12361 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    12421 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    12481 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    12541 agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc ccaatccaga
    12601 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    12661 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    12721 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    12781 gccctaacgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    12841 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    12901 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    12961 tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    13021 gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct gcggaaaatc
    13081 cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    13141 ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat tgctggttta
    13201 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgcaaaat
    13261 cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    13321 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    13381 tgctttcggt gccatctcta aaggtgctca acgctggctg gacctcggta ttgttcgttt
    13441 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    13501 cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga tatttatgcc
    13561 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    13621 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    13681 ggtagcggcg ttcattccga ttctgtggtt cttcctgatg catgattacc agcgccagcg
    13741 cgtaatgatg ctcctggacc cggaatcaga cccactaggc gcgggctatc acattattca
    13801 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc acggcactca
    13861 gtcacagctt gaatttctcc ccgaacgcca taccgacttt atcttcgcgg tactggcgga
    13921 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    13981 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    14041 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    14101 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    14161 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    14221 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    14281 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    14341 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    14401 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    14461 gatttagcca ggcgggactg gcggcaatct atgatgccga acctggcagt aacctgacgg
    14521 cctctggcga agctttcgat ccgacacagt tgacggcggc ccatccaacg cttccgatcc
    14581 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    14641 atcgcggccc ctacggcaac gaccgcgtaa tttcgctttc tcgcgcagca gctgaccgtc
    14701 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    14761 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    14821 cacctcccga tttaagcggt ggcgcggggg caagttcagt gtctggcccg cagggtgaca
    14881 ttcttccggt cagtaactcg acgctaaaaa gcgaagatcc gaccggcgcg ccggtaacca
    14941 gcagcggttt cctcggcgca ccaacgacct tagcgcctgg tgtactggaa agcagcgaac
    15001 cgacacctgc accacagccc gttgttacag ctccgtcgac aacgcctgca acctcgcctg
    15061 caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg gtgcaagtcg
    15121 gggccgttag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    15181 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg tatccagctt ggcccattcg
    15241 ccagtaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    15301 catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    15361 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    15421 ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc
    15481 gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct
    15541 gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg
    15601 gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat
    15661 gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca
    15721 atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc
    15781 accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt
    15841 ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc
    15901 atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa tagctatgtt
    15961 aacgccctgg gcctgaaaaa cacccacttc cagacggtac atggtctgga tgctgatggt
    16021 cagtacagct ccgcgcgcga tatggcgctg atcggccagg cgttgatccg tgacgtaccg
    16081 aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac
    16141 cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact
    16201 gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg cttgatctct
    16261 gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgttaacc
    16321 tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga gttcgcctct
    16381 gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac
    16441 ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct gaacagcagt
    16501 gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat
    16561 ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc
    16621 ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg
    16681 aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa aactcccgcc
    16741 ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa accaaactta
    16801 acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag gcgttacctg
    16861 agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac tacaccccaa
    16921 cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc aacgccactc
    16981 atatcgagca ggttgaaaca ctgtatgaag aactgggcaa aatcgatatt gtccgcatgg
    17041 ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct ctcctcgctg
    17101 tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt gtatcaggat
    17161 aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca ggctatgcat
    17221 gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt cgagcactat
    17281 ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc gggtgatatt
    17341 ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg acaacaggtg
    17401 atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact ggtgaccttg
    17461 cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca tcctcgggct
    17521 gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt acgaattcga
    17581 cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc accattttta
    17641 cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca atggaaaccc
    17701 gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc gctactaaac
    17761 aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca atttacactg
    17821 ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt atattttctt
    17881 tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat tgattaacct
    17941 attgaaatag ttgattatgg tgagca
//
LOCUS       DAPHJE010000093        17345 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPHJE010000093 DAPHJE010000000
VERSION     DAPHJE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.094"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            140..601
                     /locus_tag="RH222_003226"
     CDS             140..601
                     /locus_tag="RH222_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HDV3798207.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(666..1427)
                     /gene="pphC"
                     /locus_tag="RH222_003227"
                     /pseudo
     CDS             complement(666..1427)
                     /gene="pphC"
                     /locus_tag="RH222_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
     gene            complement(1424..2083)
                     /locus_tag="RH222_003228"
     CDS             complement(1424..2083)
                     /locus_tag="RH222_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV3798208.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(2304..2363)
                     /gene="ibsA"
                     /locus_tag="RH222_003229"
     CDS             complement(2304..2363)
                     /gene="ibsA"
                     /locus_tag="RH222_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV3798209.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(2636..2692)
                     /locus_tag="RH222_003230"
     CDS             complement(2636..2692)
                     /locus_tag="RH222_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3798210.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            2971..4218
                     /gene="mdtA"
                     /locus_tag="RH222_003231"
     CDS             2971..4218
                     /gene="mdtA"
                     /locus_tag="RH222_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV3798211.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            4218..7340
                     /gene="mdtB"
                     /locus_tag="RH222_003232"
     CDS             4218..7340
                     /gene="mdtB"
                     /locus_tag="RH222_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV3798212.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            7341..10418
                     /gene="mdtC"
                     /locus_tag="RH222_003233"
     CDS             7341..10418
                     /gene="mdtC"
                     /locus_tag="RH222_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV3798213.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            10419..11834
                     /locus_tag="RH222_003234"
     CDS             10419..11834
                     /locus_tag="RH222_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3798214.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            11831..13234
                     /gene="baeS"
                     /locus_tag="RH222_003235"
     CDS             11831..13234
                     /gene="baeS"
                     /locus_tag="RH222_003235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV3798215.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSELGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            13231..13953
                     /gene="baeR"
                     /locus_tag="RH222_003236"
     CDS             13231..13953
                     /gene="baeR"
                     /locus_tag="RH222_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV3798216.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCQEIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            14144..14476
                     /locus_tag="RH222_003237"
     CDS             14144..14476
                     /locus_tag="RH222_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV3798217.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            14685..14981
                     /locus_tag="RH222_003238"
     CDS             14685..14981
                     /locus_tag="RH222_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV3798218.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            14983..15279
                     /locus_tag="RH222_003239"
     CDS             14983..15279
                     /locus_tag="RH222_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3798219.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            15382..16743
                     /gene="yegQ"
                     /locus_tag="RH222_003240"
     CDS             15382..16743
                     /gene="yegQ"
                     /locus_tag="RH222_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV3798220.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(17074..>17345)
                     /locus_tag="RH222_003241"
     CDS             complement(17074..>17345)
                     /locus_tag="RH222_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798221.1"
                     /translation="GCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRIS
                     GAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFKCGE"
BASE COUNT         4041 a         4592 c         4594 g         4118 t
ORIGIN      
        1 atttctatat ggggaaaacc cactcaagaa taaaaatgat aatgcaattt tatcaggata
       61 caattcaatt tcggcctgat aatgattaac ttccacaggt ttttgctaac tttgactaca
      121 aagggattct ggagaaaaaa tgaaaaagat attacttatg ttgagtctgc tattttttac
      181 taccgcgggc tttagtgaag tttcggatac gttagtgacg ggaggttatg ataaacaagc
      241 gatgtctgac gcgattaaac atgcgcggaa agaaaccgat aaattcatcg aggttatgaa
      301 taaaaaagat gccgatactt tcgcagtcaa agccccgata accgatcacg gaagaaccga
      361 gcatttctgg cttacggatg tcacttattc taacggcatg tttattggcg taatcagcaa
      421 tgaccctggt attgttacaa acgtagaata tggccaggag tggaaaatca agaaagaaga
      481 tatttcagac tggatgtata cgcgcgggga taaaatttac ggcggttata ccattgatcc
      541 tttattggtc acctatccaa aagaagaagc cgacgagcta agagcaaaat tagttcgcta
      601 atcagccgtt atatttattg tttaaactgg cggttgaatt gttgtcttca accgccatga
      661 gaaactcact ctgcccacag cgccagtgcc aacgttttat catcatccgt acgctcgttt
      721 accgccgggc tggacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc
      781 gttgctgccg ccagtccatt gaaaaacggg gtaaagaaag gcacatgagg agaattatcc
      841 agcatattta acgccagtcg ctggatacca tccgtaaatg ctgcaacttt gtgcgcacgc
      901 ccagtgctgg tgaaagtttc cagtctggaa acggcatctt cgtcggtaat gaagtgcgtc
      961 atgttggcat attcacccgc catcggcgtg agaggcaatt gcagaccgtg acccaaatct
     1021 accaccacgc cgccatcgcc aatctgcata atcaaggtgc cgtcaggcga tgaaatcagt
     1081 cccagaaatg tgcaggcaaa atcgcgaacc gctaattctt tggcttccgc ctcggcaaaa
     1141 agtcgttgac gaatggtcag taccatattt gtggcgagga catcattaag ccccaattcc
     1201 ccgccctgca ctttttgcta catataagcc atcgcttcat tgacggcgag catcgcgcct
     1261 tcgccaccct gcgagacact gcctgcgcca tcagcgacaa ataccgacaa taatggctgc
     1321 tggtcattta accaggcaat ctgcatctgg caggcatcct gacaaggcaa gtccgcgctg
     1381 atgtgcgacg ttccaacggt tgatgcatag accagacgcc agctcacact gacgtccagc
     1441 cttttggcgc ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg
     1501 aactggataa ccagctaaac agttcacgga attgtaatcc ttgcagaggc aaaggctgac
     1561 gaacgctaat ttgtgccagc gtcttcatat ccgccccctg aacaccgatg gaaaagaaag
     1621 caaacctttt atcttcttcc ccccgaaaca ctttgttggc tgcagcctgc cactcatcgg
     1681 ttggtgcacc atcggtaatc aggaaaatcc acgggcgata atacgaaata ccatttgcac
     1741 gatactcacg tttccgctcc tctaccatat ccagggcttt ggtaatggcc gcacccattg
     1801 gtgtatcgcc ctgggcaaac aggatgggcg gaaaaaaatt agcggcgctg gtaaagggtt
     1861 gttccacatg caccgggccg aacgtcacaa tgccgagttc cactcttttt aaagccagcg
     1921 gatcagcaag cagttcatca cgaaaggtaa ccaatccggc gttaagttca ttaataggtc
     1981 taccgttcat agaaccagaa acatccagca gcaaaatgca ggggcaacgt ggttccgggt
     2041 tactggcaaa atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag
     2101 agaattatcg aggtccggta tcataccgac tgcaataatg aaataagagc aaacgaagaa
     2161 actttcagaa ttaggcccgg cggcgcagaa aattttgcta ttcgcctcgc aagaacggag
     2221 agccgtcgat tgacactaag ggcggagtga cataatttca ggagtgaggg ttagggagag
     2281 gtttccccct ccccctggtg ttcttagtaa gcctggaagc taatcactaa gagtatcacc
     2341 agtatgatga cgtgcttcat cataaccctt tccttattaa aagccctctt ctccgggaga
     2401 ggctttcccg tttcagcgtc ccgctgaaat catcggctta cctcctttcg ccctgaatgc
     2461 agtctatcgc taacgcgtga ataaaatagt ttcctctgtg attattgcgt aacgactcgc
     2521 aaagaatatt tgctggcagg atcgcagact acaaagcctg cggattgaca atcttatcgt
     2581 gaaggcatac tttcaggagt gagggtagag cggggtttcc cccgccctgg tagtcttagt
     2641 aagcggggaa gcttatgact aagagcacca cgatgatgag tagcttcatc atgacccttt
     2701 ccttatttat ggccccttcc tcgggagggg ctttcccgtt tcagcgtccc gctgaaatcg
     2761 tcggcttacc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat aaaatagttt
     2821 cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg attccagaca
     2881 gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt atcattccgc
     2941 gaaacgtttc aggaagagaa actcttaacg atgaaaggta gttataaatc ccgttgggta
     3001 atcgtaatcg tggtggttat cgccgccatc gccgcattct ggttctggca aggccgcaat
     3061 gactcccgga gtgcagcccc aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt
     3121 cgacgtggta tgcgttccgg cccattagcc ccggttcagg cggcgaccgc cgtagaacag
     3181 gcagttccgc gttacctcac cgggcttggc accattaccg ccgctaatac cgttacggtg
     3241 cgcagccgcg tggacggcca actgatagcg ttacatttcc aggaaggcca gcaggtcaaa
     3301 gcaggcgatt tactggcaga aattgacccc agccagttca aagttgcatt agcacaagcc
     3361 cagggccaac tggcaaaaga taaagccacg cttgccaacg cccgccgtga cctggcgcgt
     3421 tatcaacaac tggcaaaaac caatctcgtt tcccgccagg agctggatgc ccaacaggcg
     3481 ctggtcagtg aaaccgaagg caccattaag gctgatgaag caagcgttgc cagcgcgcag
     3541 ctgcaactcg actggagccg gattaccgca ccagtcgatg gtcgcgttgg tctcaagcag
     3601 gttgatgttg gtaaccaaat ctccagtggt gataccaccg ggatcgtggt gatcacccag
     3661 acgcatccta tcgatttagt ctttaccctg ccggaaagcg atatcgctac cgtagtgcag
     3721 gcgcagaaag ccggaaaacc gctggtggta gaagcctggg atcgcaccaa ctcgaagaaa
     3781 ttaagtgaag gcacgctgtt aagtctagat aaccaaatcg atgccactac cggtacgatt
     3841 aaagtgaaag cacgctttaa taatcaggat gatgcgctgt ttcccaatca gtttgttaac
     3901 gcgcgcatgt tagtcgacac cgaacaaaac gccgtagtga tcccaacagc cgccctgcaa
     3961 atgggcaatg aaggccattt tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat
     4021 ctggtgacgc cgggcattca ggacagtcag aaagtggtga tccgtgcagg tatttctgcg
     4081 ggcgatcgcg tggtgacaga cggcattgat cgcctgaccg aaggggcgaa agtggaagtg
     4141 gtggaagccc agagcgccac tactccggaa gagaaagcca ccagccgcga atacgcgaaa
     4201 aaaggagcac gctcctgatg caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc
     4261 tgtttattat gcgtcctgtg gccaccacgc tgctgatggt ggcgatctta ctcgccggga
     4321 ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga agtggactat ccgaccattc
     4381 aggtggtcac gctctaccca ggtgccagcc cggatgtcat gacctctgcc gttaccgcgc
     4441 cgctagaacg ccagttcggg cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg
     4501 gcggtgcgtc agttatcact ttgcagttcc agctaacatt accgctcgat gtcgccgagc
     4561 aggaagtgca ggccgcgatt aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc
     4621 cgccggttta cagcaaagtg aacccggcag atccgccgat catgacgctc gccgtcacct
     4681 caaccgccat gccgatgacg caagtggaag atatggtgga aacccgcgtc gcgcagaaaa
     4741 tctcgcagat ttccggcgtc ggcctggtga cgctttccgg cggtcagcgt ccggctgttc
     4801 gcgtcaaact taacgctcag gcgattgccg ccctcggcct gaccagcgaa accgtgcgca
     4861 ccgccattac cggcgctaac gttaactcgg caaaaggtag cctcgacggc ccttcccgtg
     4921 cggtcacgct ttccgcgaac gaccagatgc aatccgccga agagtatcgc cagctaatca
     4981 tcgcctacca gaacggcgcg ccaattcgtc tgggcgatgt cgcaactgta gagcaaggtg
     5041 cagaaaacag ctggctcggc gcgtgggcga acaaagaaca ggccattgtg atgaatgttc
     5101 agcgccagcc cggtgctaac attatctcca ccgccgacag cattcggcag atgctgccac
     5161 agctcactga gagtctgccg aaatcggtga aggtgacagt gctttccgat cgcaccacca
     5221 atatccgcgc atccgtcgat gatactcagt ttgaattgat gatggctatc gcgctggtag
     5281 tcatgattat ctacctgttt ttgcgcaata ttccggcgac catcattccc ggtgttgctg
     5341 taccgctgtc gttaatcggc actttcgcgg ttatggtgtt tctcgatttt tcaatcaata
     5401 acctgacact gatggcgtta actatcgcca ccggattcgt ggtcgatgac gccatcgtgg
     5461 tgatcgaaaa catttcccgc tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca
     5521 agggcgcagg tgaaatcggc tttaccatta tctcgctgac cttctcactg attgcggtgt
     5581 tgatcccact gctgtttatg ggcgatatcg tcgggcgact gttccgcgaa tttgctatta
     5641 ccctggcggt agcgattttg atctcagcgg tggtgtcgct gaccctgaca ccgatgatgt
     5701 gcgcgcggat gctcagccag gagtcgttgc gtaaacagaa ccgcttctcc cgtgcctcgg
     5761 aaaaaatgtt cgacaggata atcgccgcct atggtcgtgg actggcgaaa gtgctgaatc
     5821 atccgtggct gaccttaagc gtggcactca gcacgctgct gcttagcgtg ctgctgtggg
     5881 tgttcattcc gaaaggtttc ttcccggtac aggacaatgg cattattcag ggcactttgc
     5941 aggcaccgca atccagctcc tttgccaata tggcccagcg acaacgccag gtcgcggacg
     6001 tgattttgca ggatccggca gtgcaaagcc tgacctcatt tgttggcgtt gatggcacta
     6061 acccgtcgct gaacagtgca cgtttacaaa tcaacctcaa accgttggat gaacgtgatg
     6121 atcgggtgca aaaagtcatc gcccgtctgc aaacggcggt agataaagtg ccgggcgtcg
     6181 atctcttcct gcaaccaacg caggatctga ctattgatac tcaggtcagc cgcacccagt
     6241 accagtttac cttgcaggcc acgtcactgg atgcgctcag tacctgggtg ccacagttga
     6301 tggaaaaact ccagcaactg ccacagcttt ctgatgtctc cagcgactgg caggacaaag
     6361 ggctggtggc gtatgtcaat gttgatcgcg acagcgccag ccgtctgggg atcagcatgg
     6421 cggatgtcga taacgccctg tacaacgcgt ttggtcagcg gctgatttcc actatttata
     6481 ctcaggccaa ccagtatcgc gtggtgctgg agcacaacac cgaaaatacc ccaggcctcg
     6541 cggcgctgga taccattcgc ctgaccagca gcgacggcgg cgtggtgccg ctaagctcaa
     6601 ttgccaaaat tgagcagcgt tttgcgccgc tctccatcaa ccatctggat cagttcccgg
     6661 taacgaccat ctcctttaac gtgccggata actattcgct gggcgatgcg gtgcaggcga
     6721 ttatggacac cgaaaagacg ctgaatctgc cggtggatat caccacgcag ttccagggca
     6781 gcaccctcgc cttccagtcg gcgctgggca gcactgtctg gctgattgtc gcggcggtgg
     6841 tggcgatgta tatcgtgctc ggcattctgt acgagagctt tattcacccg atcaccattc
     6901 tctcgacgct acccaccgca ggggttggcg cactgctggc gttgctgatt gctggtagcg
     6961 aactggatgt gattgcgatt atcggcatta ttttgctgat cggtatcgtg aagaagaacg
     7021 ccatcatgat gatcgacttc gcgctggctg ccgagcgcga gcaaggcatg tcgccgcgcg
     7081 aggcaatcta ccaggcttgt ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg
     7141 ctctgcttgg cgcgctgccg ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc
     7201 cgttaggtat cggcatggtc ggcggcctga ttgtcagcca ggtgctgacg ctgttcacca
     7261 cgccggtgat ttatctgctg ttcgaccgcc tggcattgtg gaccaaaagc cgctttgccc
     7321 gtcatgaaga ggaggcgtaa gtgaagtttt ttgccctctt catttaccgt ccggtggcga
     7381 cgattttact gtcggttgcc attaccctgt gcggcatact gggcttccgt atgctgccgg
     7441 tcgccccgct gccgcaggtc gattttccgg tgattatggt cagcgcctcg ctgcccggtg
     7501 cgtcaccaga aacaatggcg tcttccgttg ccacgccgct ggagcgctca cttgggcgca
     7561 ttgccggagt cagtgaaatg acctccagca gttcgctcgg cagcacgcgt attattttgc
     7621 agtttgattt tgaccgggat atcaacggcg cagcgcgtga tgtgcaggcg gcgatcaacg
     7681 ctgcacaaag tttgctgccc agtgggatgc ccagccgccc gacctatcgc aaagcgaacc
     7741 cgtcggatgc gccaattatg atcctcacgc tgacgtccga tacttattcg cagggtgaac
     7801 tgtacgattt cgcctcgacg cagctggctc cgacgatttc gcaaatcgac ggtgttggtg
     7861 atgtcgatgt cggaggcagc tcactgcccg ccgtacgcgt cgggctgaat ccgcaggcgc
     7921 tgtttaatca gggcgtgtcg ctggacgacg tacgcaccgc cgtcagcaat gccaacgtgc
     7981 gtaaaccgca gggcgcgctg gaagatggca ctcaccgctg gcagatccag accaatgatg
     8041 agctaaaaac cgccgctgaa tatcagccgt tgattattca ctacaacaac ggcggcgcgg
     8101 ttcgtctggg cgatgtggcg acggtgaccg actcagtgca ggatgtgcgc aacgccggga
     8161 tgaccaacgc caaaccggct attttactga tgatccgcaa actgccggaa gccaatatta
     8221 tccagacggt tgacagcatc cgggcaaaat taccggagtt gcaggaaacc attccggcgg
     8281 cgattgatct gcaaattgcc caggatcgct cccccaccat tcgcgcctcg ctggaagaag
     8341 tcgagcaaac gctgattatc tcggtggcgc tggtgattct ggtggtgttt ttattcctgc
     8401 gctcgggtcg cgccactatt attcccgccg tttcggtgcc ggtttcgctg attggtacgt
     8461 ttgcggcgat gtacctgtgc ggattcagtc tcaataacct ttcgttaatg gcgctcacca
     8521 tcgctactgg tttcgtggtg gatgacgcca tcgtggtgct ggaaaacatt gcacgtcatc
     8581 tggaagcggg aatgaaaccg ttgcaagccg cactgcaagg tactcgcgaa gtcggtttta
     8641 cggtgctgtc gatgagtctg tcactggtgg cggtgttcct gccgctgctg ttgatgggcg
     8701 gattgccggg ccgactgtta cgcgaatttg ccgtgacgct ttctgtcgcc attggtatat
     8761 cgttgctggt ttctctgaca ttaacgccaa tgatgtgtgg ctggatgctg aaagccagca
     8821 agccgcgcga gcaaaagcga ctgcgtggtt ttggtcgcat gttggtagcc ctgcaacaag
     8881 gctacggcaa gtcactgaaa tgggtgctca atcatacccg tctggtgggc gtggtgctgc
     8941 ttggcaccat tgcgctgaat atctggctgt atatctcgat cccgaaaacc ttcttcccgg
     9001 agcaggacac tggcgtgttg atgggcggga ttcaggcgga tcagagtatt tcgtttcagg
     9061 cgatgcgcgg taagttgcag gatttcatga aaattatccg tgacgatccg gcagtggata
     9121 atgtcaccgg ctttacaggc ggttcgcgag tgaacagcgg gatgatgttt atcaccctca
     9181 agccacgcga cgaacgcagc gaaacggcgc agcaaattat cgaccgtctg cgcgtaaaac
     9241 tggcgaaaga accgggggcg aatctgttcc tgatggcggt acaggatatt cgcgttggtg
     9301 ggcgtcagtc gaacgccagc taccagtaca cgttgttatc cgacgacctg gcggcactgc
     9361 gcgaatggga gccgaaaatc cgcaaaaaac tggcgacgtt gccggaactg gcggacgtga
     9421 actccgatca gcaggataac ggcgcggaga tgaatctggt ttacgaccgc gacaccatgg
     9481 cacggctggg aatcgacgta caagccgcca acagtctgtt aaataacgcc ttcggtcagc
     9541 ggcaaatctc gaccatttac cagccgatga accagtataa agtggtgatg gaagtggatc
     9601 cgcgctatac ccaggacatc agtgcgctgg aaaaaatgtt cgttatcaat aacgaaggca
     9661 aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc gaatgcccca ctatcggtga
     9721 atcatcaggg attatcggcg gcctcgacca tttcgtttaa cctgccgacc ggaaaatcgc
     9781 tctcggacgc cagtgcggcg atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg
     9841 tgcgcggcag ttttgccggc acggcgcagg tgttccagga gacgatgaac tcgcaggtga
     9901 tcctgattat cgccgccatc gccacggtgt atatcgtgct gggtatcctt tacgagagtt
     9961 acgtacatcc gctgacgatt ctctccaccc tgccctcggc gggcgttgga gcgctgttgg
    10021 cgctggagct gttcaatgcc ccgttcagcc taatcgccct gatagggatc atgctattaa
    10081 tcggcatcgt gaagaaaaac gccattatga tggtcgattt tgcgcttgaa gcccaacggc
    10141 acggtaacct gacgccgcag gaagctattt tccaggcctg tctgctgcgt tttcgcccga
    10201 ttatgatgac caccctggcg gcgctgtttg gtgcgctgcc gctggtattg tcgggcggcg
    10261 acggctcgga gctgcggcaa cccctgggga tcaccattgt cggcggactg gtaatgagcc
    10321 agctccttac gctgtatacc acgccggtgg tgtatctctt tttcgaccgt ctgcggctgc
    10381 gtttttcgcg taaacctaaa caaacggtaa ccgagtaaat gacagatctt cccgacagca
    10441 cccgttggca attgtggatt gtggctttcg gcttctttat gcagtcgctg gacaccacca
    10501 tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc
    10561 acatggtcat tgtctcttat gtgctgaccg tggcggtgat gctgcccgcc agcggctggc
    10621 tggcggacaa agtcggcgtg cgcaatattt tctttaccgc catcgtgctg tttactctcg
    10681 gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact gttgctggca cgcgcgttac
    10741 agggcgttgg cggcgcgatg atggtgccgg tcggcagatt gacggtgatg aaaatcgtac
    10801 cgcgcgagca atatatggcg gcgatgacct ttgtcacatt acccggtcag gtcggtccgc
    10861 tgctcggtcc ggcgctcggc ggtctgctgg tggagtacgc gtcgtggcac tggatctttt
    10921 tgatcaacat tccggtgggg attatcggtg cgatcgccac attgctgtta atgccgaact
    10981 acaccatgca gacgcggcgc tttgatctct ccggattttt attgctggcg gttggcatgg
    11041 cggtattaac cctggcgctg gacggcagta aaggtacagg tttatcgccg ctgacgattg
    11101 caggcctggt cgcagttggc gtggtggcac tggtgcttta tctgctgcac gccagaaata
    11161 accaccgcgc tcttttcagt ctgaaactgt tccgcactcg taccttttcg ctgggcctgg
    11221 cggggagctt tgccggacgt attggcagtg gcatgttgcc ctttatgaca ccggttttcc
    11281 tgcaaattgg cctcggtttc tcgccttttc atgccggact gatgatgatc ccgatggtgc
    11341 tcggcagcat ggggatgaag cgaattgtgg tacaggtggt gaatcgcttt ggctatcgtc
    11401 gggtactggt ggcaaccacg cttggcctgt cgctggtcac cctgttgttt atgactaccg
    11461 ccctgctggg ctggtactac gttttgccgt tcgtcctgtt tttacaaggg atggtcaact
    11521 cgacacgttt ctcctccatg aacaccctga cgctgaaaga tctcccggac aatctggcga
    11581 gcagcggcaa cagcctgctg tcgatgatta tgcaattgtc gatgagtatc ggcgtcacta
    11641 tcgccgggct gttgctggga ctttttggtt cacagcatgt cagcgtcgac agcggcacca
    11701 cacaaaccgt ctttatgtac acctggctta gcatggcgtt gatcatcgcc cttccggcgt
    11761 tcatctttgc cagagtgccg aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa
    11821 ggagcgcgca atgaagttct ggcgacccgg tattaccggc aaactgtttc tggcgatttt
    11881 cgccacctgc attgtcttgc tgatcagtat gcactgggcg gtgcgtatca gttttgagcg
    11941 tggctttatt gattacatca agcatggtaa tgaacagcga ttacaactgt taagtgatgc
    12001 gcttggcgag cagtatgcgc agcatggcaa ctggcgcttc ctgcgcaaca atgatcgctt
    12061 tgtctttcag atcctgcgtt catttgaaca cgataattcg gaagataaac ccggcccggg
    12121 tatgccaccg cacggctggc gtacccagtt ctgggtggtt gatcaaaaca acaaagtgct
    12181 ggttggtccg cgagcgccga ttccacctga cggtacacgg cgacccattc tggtcaacgg
    12241 tgcggaagtt ggcgcggtga tcgcctcccc cgttgagcgg ttaacgcgca atactgatat
    12301 caatttcgat aaacaacagc ggcaaaccag ctggttgatt gtcgccctgg caacgttact
    12361 cgcggcactt gccacttttc tgctggcgcg cggtttactg gcaccggtaa aacgacttgt
    12421 cgatggcacg cacaaactgg cggcgggcga tttcactacc cgcgtaacgc ccaccagtga
    12481 agatgaactg ggcaaactgg cgcaagactt caaccagctt gccagcacac tggagaaaaa
    12541 ccagcaaatg cggcgcgatt ttatggccga tatttctcac gaactgcgta cgccattagc
    12601 ggtgctgcgc ggtgaactgg aagccattca ggatggcgtg cgtaaattca cgccggagac
    12661 ggtggcgtct ttacaggcgg aggtcggtac actgaccaaa ctggttgacg atctccatca
    12721 gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc
    12781 actgctggaa gtggcgggcg gcgcatttcg cgaacgattc gccagtcgtg gcctgaaact
    12841 gcaattttcc ctgccagaca gtattaccgt atttggcgat cgcgaccgtt taatgcagtt
    12901 attcaataac ttactggaaa acagcctgcg ctacactgac agcggcggca gcctgcaaat
    12961 ctctgccggg cagcgcgaca aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt
    13021 cagtgacgat cagctacaaa aattgtttga acgtttttat cgcaccgaag gttcccgcaa
    13081 ccgtgccagc ggcggttccg agctggggct ggcgatttgc ctgaacattg ttgaagcaca
    13141 taatggtcgc attattgctg cccattcgcc ttttggcggg gtaagcatta cagtagagtt
    13201 accgctggaa cgggatttac agagagaagt atgaccgagt taccaatcga cgaaaacaca
    13261 ccgcgtattt tgatcgtgga agatgaaccg aagctggggc agttgctcat tgattatctg
    13321 cgtgctgcga gctatgcgcc gacgcttatc agccacggcg atcaggtact gccgtatgtg
    13381 cgccagacac caccggatct gatcctgtta gatctgatgc tccctggcac cgatggcctg
    13441 acgctgtgcc aggaaattcg tcgtttttct gacattccga tcgtgatggt gacggcaaaa
    13501 atcgaagaga tcgatcgcct gctggggctg gagattggcg cagatgatta tatctgtaag
    13561 ccgtacagcc cacgggaagt ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg
    13621 cagcgcgagt tgcagcaaca ggatgctgaa agcccgttga ttatcgacga aggtcgtttt
    13681 caggcttcat ggcgcggtaa aatgcttgac ctgacgcctg cggaatttcg tctgctgaaa
    13741 acgctctctc atgaaccagg aaaagtgttc tcccgcgagc atttgctcaa tcatctttat
    13801 gacgactacc gcgtagtaac cgaccgcacc atcgacagcc acattaaaaa cctgcgccgc
    13861 aagctggaat ctctcgacgc cgaacagtca tttatccgcg ccgtttacgg cgtcggttac
    13921 cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc gacattattt tgttagccgg
    13981 agatggcgtt cagacccgat ccggcaacat tatcccacgc atggtcagca aactgacatt
    14041 atctctccgc cctggcaggc atcagaaaat cgctcatact ttaatcggta aacagcacct
    14101 ttagatgctg ttttcgatac acaatttcaa tcaaggagtc attatggctg gttggtttga
    14161 actcagtaag agcagtgata atcagttccg gtttgtgcta aaagcgggca atggtgagac
    14221 tatcctcacc agcgagctgt atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt
    14281 gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa acggcaagta acggcaaatt
    14341 ctatttcaat ctgaaagccg ctaatcatca aattatcggc tccagccaga tgtacgccac
    14401 cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc aatggcacaa gccagacggt
    14461 gaaagacaat acgtaattac gatgccgggc gtgggttgtt ggcgtccggc tttatatcca
    14521 aataacgctt agttattaat aattaagagt aaccgccgcc attaaaatag tgggcactga
    14581 cgagcactcg ttgtgctttc tttgtttacc atcccatact tatcctcctg aacatgttca
    14641 tgtgccaaaa tatcttctaa tactttcgtg acgctatccg gtttatgaca tacactgatg
    14701 taagattttt acttacaggc gggcgtaaaa tggccagaac gatgacggtt gatctcggcg
    14761 atgagttgcg tgaatttatc gagtccttaa ttgaatccgg cgactatcgc acccaaagcg
    14821 aagtgatccg tgagtcactg cgattacttc gagaaaagca agcagagtct cgcctgcaag
    14881 cgttacgtga catgctcgca gaagggttga gtagtggtga agctcagccg tgggaaaagg
    14941 acgcgttctt acggaaggtg aaagcaggga taaggaaata atgtgcgtat tattaaactc
    15001 atgccaaaag ccaatgagga tttagaaggc atctggtatt acagctatca ccatttcggc
    15061 gaaccacaag ccgacagata tgtggaacat ctttcagacg ttctccagat tctgagtaac
    15121 aataatatag gtacgcccag acctgaatta ggcgaaggta tttttgttct tccttttgaa
    15181 cgccacgtca tttatttcct gcaatcacca ggggaaatta tcgtcattcg tatccttaac
    15241 caaaaccagg acgccaccag acatttacac tggagctaaa cccgccgatt gcgctacaat
    15301 gcccgccctt aaagtggggg cactccccta accgcttcat caggtgaagc ggatctgacc
    15361 tgtcatcaga acgagagaat tatgtttaaa ccggaactcc tttccccggc gggaacgctg
    15421 aaaaatatgc gttacgcttt cgcttatggc gcagatgctg tttatgcggg ccagccgcgt
    15481 tattccctgc gtgtgcgcaa caacgaattc aaccacgaaa atcttcagct cggcatcaat
    15541 gaagcccacg cgctggggaa aaagttttat gtcgtggtca acattgcacc gcacaacgcc
    15601 aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg
    15661 attatgtccg atccagggct gattatgctg gtgcgtgagc acttccctga aatgccgatc
    15721 cacctttcgg tgcaggctaa cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg
    15781 ggcctgaccc gcgtgatcct ctctcgcgag ctgtcgctgg aagagattga agagatccgc
    15841 aatcaggtgc cggatatgga gatcgagatc ttcgttcacg gcgcgctgtg catggcctac
    15901 tccggtcgct gcctgctctc tggctatatc aacaagcgcg acccgaacca gggcacctgc
    15961 accaacgcct gccgctggga gtacaacgtc caggaaggga aagaagatga tgttggcaac
    16021 atcgtacaca agtacgagcc gattccggtg caaaatgttg agccgacgct gggtatcggc
    16081 gcaccaaccg acaaagtgtt tatgatcgaa gaggcccagc gtccgggcga gtatatgacc
    16141 gcgtttgaag atgagcacgg cacttacatc atgaactcga aagatctgcg cgccatcgcc
    16201 catgtagaac gcctgaccaa aatgggcgtg cattcgctga aaatcgaagg tcgtaccaaa
    16261 tctttctact attgtgcacg caccgcacag gtttaccgca aagctatcga tgacgccgct
    16321 gcgggaaaac cgttcgatac cagcctgctg gaaactctgg aaggtctggc gcatcgtggc
    16381 tataccgaag gtttcctgcg tcgtcatact cacgacgatt atcagaacta cgaatacggt
    16441 tattcagttt ctgaccgcca gcagtttgtt ggtgagttta ccggtgagcg caagggggac
    16501 ctcgcggcgg tagcggtgaa aaataaattc tccgttggcg acagccttga gctgatgacg
    16561 ccgcaaggca acattaattt tacccttgag cacatggaaa acgccaaagg cgaagctatg
    16621 ccgatagcac caggcgatgg ttatactgtg tggctcccgg tcccgcagga tcttgagctc
    16681 aattacgcgc tgctgatgcg taatttctcc ggggaaacca cgcgtaatcc ccacggtaag
    16741 tgattaattt cgattatttt tcccggatgg aaaattctta gaaaccgatc acatacagct
    16801 gcatttatta aggttatcat ccgtttcgct gaaaaacata acccataaaa tgctagctgt
    16861 accaggaacc acctccttag cctgtgtaat ctcccttaca cgggcttatt tttttatgca
    16921 taagccctat ctctggtaac cgtcttccat tgaccacatc gatagaatcc tccttcatag
    16981 cacgatgcct ttcacttatc ggcatcgtgc tcccacaggt tccggctacg cacagccaga
    17041 acgcgcatat ttgacgctta ccaaaaaata ttctcactct ccacatttga atgtcagacg
    17101 agcgacacca tgtaatccta caccttctgt cttcagctca actatttgca tttttttgcc
    17161 ctgagtaaca cagaaatgag ttgcatcatt ttttactata ttttctgcac cagatattct
    17221 acccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt tacgctgaat
    17281 attactttta tatgcaagac caaatttacc gatacttgtt tcatcattat gcacagcaca
    17341 accag
//
LOCUS       DAPHJE010000094        17148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPHJE010000094 DAPHJE010000000
VERSION     DAPHJE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.095"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..1940
                     /gene="yegI"
                     /locus_tag="RH222_003242"
     CDS             6..1940
                     /gene="yegI"
                     /locus_tag="RH222_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV3798222.1"
                     /translation="MKVFTSTGELTTLGRELGKGGEGAVYDIEEFVDSVAKIYHTPPP
                     ALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYSPA
                     HRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDSDS
                     FQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTENHDNFGLALLIFHVLFGGRHP
                     YSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQAF
                     TESGVATGRPTAKAWVAALDSLRQQLKKCIVSAMHVYPAHLTDCPWCALDNQGVIYFI
                     DLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYIIL
                     LEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRAKM
                     DYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQKF
                     LEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKAS
                     CERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMPLM
                     EPLRQAAEKLAQAQADLSRC"
     gene            complement(1952..3304)
                     /gene="yegD"
                     /locus_tag="RH222_003243"
     CDS             complement(1952..3304)
                     /gene="yegD"
                     /locus_tag="RH222_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3798223.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSTAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3438..4286
                     /gene="alkA"
                     /locus_tag="RH222_003244"
     CDS             3438..4286
                     /gene="alkA"
                     /locus_tag="RH222_003244"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV3798224.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(4395..7712)
                     /locus_tag="RH222_003245"
                     /pseudo
     CDS             complement(4395..7712)
                     /locus_tag="RH222_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            8030..8671
                     /gene="udk"
                     /locus_tag="RH222_003246"
     CDS             8030..8671
                     /gene="udk"
                     /locus_tag="RH222_003246"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV3798225.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8763..9344
                     /gene="dcd"
                     /locus_tag="RH222_003247"
     CDS             8763..9344
                     /gene="dcd"
                     /locus_tag="RH222_003247"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV3798226.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9366..11219
                     /gene="asmA"
                     /locus_tag="RH222_003248"
     CDS             9366..11219
                     /gene="asmA"
                     /locus_tag="RH222_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV3798227.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGLSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPMLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11671..13254)
                     /locus_tag="RH222_003249"
     CDS             complement(11671..13254)
                     /locus_tag="RH222_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV3798228.1"
                     /translation="MEWIADPSIWAGLVTLVVIELVLGIDNLVFIAILAEKLPPAQRD
                     RARITGLMLAMVMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAIGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEAFNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(13327..14361)
                     /locus_tag="RH222_003250"
                     /pseudo
     CDS             complement(13327..14361)
                     /locus_tag="RH222_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            15114..16004
                     /gene="galF"
                     /locus_tag="RH222_003251"
     CDS             15114..16004
                     /gene="galF"
                     /locus_tag="RH222_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GalU regulator GalF"
                     /protein_id="HDV3798229.1"
                     /translation="MANLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEIVLVTHSSKNAVENHFDTSYELEALLEQRVKRQLLAEVQAICPPGVTIMNV
                     RQAQPLGLGHSILCARPVVGDNPFVVVLPDIILDGGTADPLRYNLAAMVARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPMVAEGQVARIVEFIEKPDEPQTLDSDLMAVGRYVLS
                     ADIWAELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGESYDCGKKMGYMQAFVTY
                     GMRNLKEGTKFRESIKKLLA"
     gene            complement(16281..>17148)
                     /locus_tag="RH222_003252"
     CDS             complement(16281..>17148)
                     /locus_tag="RH222_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HDV3798230.1"
                     /translation="KYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDE
                     RLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRAT
                     TNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYA
                     MGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRK
                     GNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISP
                     AAFREKYHQMTA"
BASE COUNT         4107 a         4421 c         4548 g         4072 t
ORIGIN      
        1 caaatataaa agtatttaca tcgacaggtg aattgaccac tcttggccgt gaactgggca
       61 aaggtggtga aggcgcggtt tatgatatcg aggagtttgt cgatagcgtc gccaagattt
      121 atcacacgcc gccacccgcc ttaaaacagg acaaacttgc ctttatggct gcgacagctg
      181 acgcgcagtt gttgaattat gtcgcctggc cgcaggcaac gcttcacggt ggacgaggcg
      241 gaaaagttat cggttttatg atgccaaaag tttctggtaa agaaccgatt catatgatct
      301 atagcccggc acatcgtcgc cagagttacc ctcattgtgc gtgggatttt ctactctatg
      361 ttgcgcgcaa tattgcttca tcttttgcta cggttcacga gcacgggcac gtcgtgggtg
      421 acgtaaacca gaacagtttt atggtaggtc gcgacagcaa agtggtactg attgatagcg
      481 actcctttca aattaacgcc aatggcacac tgcatttatg cgaagtcggc gtgtcgcatt
      541 ttacgccgcc agagctacaa accttgccat catttgtcgg ttttgaacgc acagaaaatc
      601 acgataattt tggccttgcg ttgctaattt ttcacgtctt gtttggtggg cggcatcctt
      661 attctggtgt gccgcttatc tctgatgcgg gtaatgcgct ggagacggat attacccatt
      721 tccgttatgc ctacgcgtca gataatcagc gacgtggttt aaaaccgccg ccacgatcta
      781 ttccgctatc gatgttaccg agtgatgttg aagccatgtt tcagcaggca ttcacggaaa
      841 gtggcgtggc aaccgggcgt ccgacggcta aagcgtgggt agcggcactg gactctctac
      901 gccaacagtt aaagaaatgt atcgtttcag caatgcatgt ttaccccgct catttgactg
      961 actgcccgtg gtgtgcgctg gataatcaag gtgttatcta ttttattgat ctcggcgaag
     1021 aggtcattac caccggcggg aattttgtgc tggcgaaagt ctgggcgatg gtgatggcgt
     1081 cagtagcacc gccagcattg caactgccat tacccgatca tttccaaccg actggcaggc
     1141 cgcttccttt aggcctgtta cggcgcgaat acatcattct gcttgagatc gcactgtcag
     1201 cattatcgct gctgctttgc ggccttcagg cagaaccgcg ttatattatt ttggttcctg
     1261 tgctggcggc tatctggatt attggcagtc tgacaagcaa agcgtacaaa gcagaagttc
     1321 agcaacgccg tgaggcattt aatcgtgcga aaatggacta tgaccattta gtccgccaga
     1381 tccagcaggt aggcggcctg gaaggtttta tcgccaaacg gacgatgctc gaaaaaatga
     1441 aggacgaaat tctcgggtta ccggaagaag agaaacgcgc tctggcagca cttcatgaca
     1501 ctgcaaggga acggcagaag cagaagtttc tggagggatt ttttattgat gttgcctcta
     1561 ttcccggcgt tggccctgcg cgtaaagcgg cgttacggtc ttttggtatt gaaacagccg
     1621 cagatgttac ccgtcgtggc gttaagcaag tgaaagggtt tggcgaccat ctgacccagg
     1681 cggttatcga ctggaaagcg agctgtgaac gccgttttgt gttcaggccg aatgaagcga
     1741 taacgccagc agacagacaa gcggtaatgg cgaaaatgac cgccaaacga catcggctgg
     1801 aatcgacgtt gactgtcggc gcgacagagt tgcagcgatt ccgccttcat gctccagcac
     1861 ggaccatgcc gttgatggaa ccgttacgtc aggcggcaga aaaactggct caggcgcagg
     1921 ctgatttaag ccgctgctga gccattttct tttaacgaaa taccacttct gcccagcgtg
     1981 ccagcccggc ggtgacggag ccaaagtcat cgccgcctgc aatcggaatg cccggcaact
     2041 gttctgccag cgctttttta atcagcggcg aacgggcgct accgccggtc aggtagataa
     2101 cgtccggttt ttcctgggcg ttatccagcg ccagttgcac ctgttccaga atccgcgcca
     2161 gtggctggct gagggcgctt tccagccctt gctggctaat cagcgtagcc agttcatcgc
     2221 tgataaacgg cagtgaggcg cgggtttccg cagtgcttga aagggcgatt ttgctctctt
     2281 ctgcgctacg caccaggcga tagcttaaac gctgacgcca gactttctgt aacagggcca
     2341 ctttttccgg ttcgcgggca tcgcgtacca gatcgttaag cagacgaccg ttggcactac
     2401 tgtagaaatc actctgtgca ggaacgtcgt tgatggcaac cgcattccac cacggcagaa
     2461 tcggcagggc gatgcctttt tcggtttcgc cacccatgcc cagtaacggc atcaggtttt
     2521 taaacgccag cgcgatatcc agatcgttac cgccaatacg gcaaccactg tgacccagca
     2581 ggctggcttc acgatcgaga cgcgaacgcc actgcggccc catcagcagc aatgaacagt
     2641 cagtcgtacc accgccgata tccaccacca gcacccgttt ttcttcctgc aaggtggctt
     2701 cgtaatccag cccagccgcg accggctcgt actggaatac cacgtccctg aatccggcac
     2761 gcttcgccgc gcgttccaga atcccttgcg cctgggtgtt tgcttcatca ccgcccagcc
     2821 cctggaagtt gatcggacga ccaatcaccg cctgagtaat cgcttctggc agctgtgcct
     2881 gcgcctgctg gcgaatgtgc aacatcattg cgcagaccag atcctcaaac agcgctacct
     2941 gctgcggttt taagccgctg gcaccgagga acgattttgg tgatttcaca aaccacactt
     3001 cttctggatc atcaatgtac tgtgccagtg aggaaagacc gaactgcacg cttttcgccg
     3061 taacatcgat atcttcttcg cggttataac gaatcgcccg acgtaacagc gcctgcgttt
     3121 catcgtcgtc tgccggaaca tcatgatggc ggtacagcca ttcgcttacc gcttcacgcg
     3181 ttggcgcgca aagcattgaa ggcagcagcg tgctgtcgtt ttccattttt agcaaatgcg
     3241 gtttaccgtc acgcatgacc gccactgaac agtttgctgt accgtaatca aaaccaataa
     3301 acacgaaata atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa tgttgccgtc
     3361 gcgacaaccg gaatatgaaa gcaaagcgca gcgtctgaat aacgtttatg ctgaaagcgg
     3421 attaacaagg agatgcgatg tataccctga actggcagcc gccgtatgac tggtcgtgga
     3481 tgttgggatt tctcgccgcc cgtgcggtga gcggtgtgga aacggtcgcg gacagttatt
     3541 atgcccgtag tctggcggtg ggcgaatatc gcggcgtggt gactgctatt ccggatatag
     3601 cccgccatac tctgcacata aatttaagtg caggtttaga acctgttgcc gcagagtgtc
     3661 tggcgaaaat gagccgcctg tttgatctgc aatgtaaccc acaaattgtt aacggtgcgt
     3721 tgggcaggtt aggcgcggcg cggcccggat tgcgtttacc cggctgtgtt gatgcttttg
     3781 agcagggcgt gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg gcaaaattga
     3841 ccgccagagt ggcacagctt tatggcgaac ggctggatga ttttccggag tatatctgct
     3901 tcccgacgcc tcagcggctg gcagcagcag acccgcaggc attaaaagcg ttaggtatgc
     3961 cgttgaaacg ggcagaggcg ctgattcatc tggcaaatgc ggcgctggag ggcaccttac
     4021 caatgacaat accgggcgat gtggagcagg cgatgaaaac gctgcaaact tttccgggta
     4081 tcgggcgctg gacggcgaat tattttgctt tgcgtggctg gcaggcgaaa gatgttttcc
     4141 tgccggatga ttatctgatt aaacagcgat ttccgggaat gacaccggcg caaatccgcc
     4201 gttatgccga gcgctggaag ccctggcgtt cttatgcgct gttgcatatc tggtatacgg
     4261 aaggctggca accagacgaa gcatgacgtt ggtcgccgga tgcggtgcga gcgccttatc
     4321 cggcctgggg aatgagcgat ttgtaggcat gataagacgc gttagcgtcg catcaggcga
     4381 tggggaagca cgcctcagtt aatcgcgaaa taactactat tcaccagcaa atccagcggt
     4441 tgggcattgg caatcacatc accataaatc agatcgacgc cgatgcccga aagcgtatcc
     4501 atcactaagg gtaaaacgac tggcccggcg atggttttca tcccgagtcg ctgagcgtgc
     4561 ccctgaataa tcgtaatcag catctcatcc atcaaattac cctgcacgtt ggcgcataac
     4621 tcaccatcaa gtagcaggta atctgccata tttgctttca gcgagttgaa gatttgcaga
     4681 tcgcggccca tctgactgag cactatccga catcccgcca gccgcagttt ttgcacactt
     4741 tctgcgtgat cgaaaatcgc ttctgccgga ataatcagat gtaataaccg tgctggtaga
     4801 gggctatttt cccgctgctc aagcagttca ttcaccagcg tggcgctact caaaccagca
     4861 acggaaaggg ggagggcgat gcttaagcct ttactggcaa ccgcttttgc ggcctgctgg
     4921 aaaaaatcgt ggaatacccg tcggtcaaga gcatggctaa gtgccggatc gctgaagcta
     4981 cgacgaaatg tttgttcatc aataatctcg ctttcgcaac tccagagctt aagtgaaatc
     5041 agccacaaat tacgcgcttc cgggatccgt ggcgaagcga caccgtgggc gagcatcatc
     5101 aactgattct ctttaatcat ccgccactgt tcatcaagcg acatcgccgc ccgttcgcta
     5161 tgtgcggcag cttgctgcgg ttcgtaaacc gtcacccggc cccggccacc atttttggag
     5221 gcataacagg cgatatcagc ctgcgacatc acttctgccg cctgatgatt gttgtcatca
     5281 atcaaggtaa tcccggcact ggcacctacc cgatgcacac ggccttccca tataaagtga
     5341 tagtcattca cggcactgat aatgcgtgta gcgataaaac gcgcgctttc aacattacag
     5401 tctggtagca gcagaccaaa ttcatcccca ccgagtcgcg ccagcacgtc gctggaacgc
     5461 agcatactca gcattaacga cgccagttcg cgcagcaaag cgtcacccgc cgcatgcccg
     5521 gcgctgtcat tcaccgcttt aaagcgatca agatcgataa acaccagtgc atgtcgctga
     5581 tgtgtactgt ttaccgtttg cagcaggatg cgtagttgct tctcaaaact ggcgcgattg
     5641 gcgagatgcg tcagtgcatc atgggaggcg ctgtagctca gctggcgcag cattttgcgc
     5701 gattcggtga cgtcctgaat caccagaacc gaaccaatat tgctgccgtc cagagtactt
     5761 aacggcgtaa tactgtaatg cacgtcgtag ctgccgccgc ttcggcagtg caacaccaca
     5821 tcttgttcaa tcgcggaacg tgaggtgtcg gcactgtaaa tgttctccat taatggtccg
     5881 ttgtcgccaa aagtaatatg caacaccgtc aggagtggaa cacctaacgc ttcttcctgc
     5941 gtccagccgc tcatcttctc cgccactgga ttcataaagg taattttcat cgccatatca
     6001 atacagacca cggcttcacc gatggaatca agcgtaatgt gcaggcgctc tttttcctga
     6061 aacaatgcct cgttaagctg tttaacctcg gtcatatcca tattaatgcc gagcagacgt
     6121 tcgacttcgc cttctttatt cagttcccgg ttggcgaggg cgcggatatg gcggatgcca
     6181 tctttcacgg caatgcgaaa ttccagttta aagggagagc gcgattgcaa tgaatcgcga
     6241 atcacttttt cggcgtgctg gcgatcttcc ggcagcacgc actcgtacca cacctgccag
     6301 ttcggtttga tatgcggagg aatttcatac agctcgaaca tccgcttatc ccagctataa
     6361 atattcggct taagctccca ctcccagatg ccaatcccgc ccgcttcgtt agccagcgtg
     6421 atgcgctcca tcagttgctg attcacctgt tcggtgcgtt ttagctcgtt aatgtcttca
     6481 atctgagcga taaaatagag cggcgtacca tcggtgtggc gcaccagtga gacggcaagc
     6541 aacgcccaga caacatcgcc attgcggttg tagtagcgtt tttccattga ataggtgttt
     6601 atttcaccgc tgatcagctt ttcaacctgt tgcagatctt tattgagatc ctccggccag
     6661 gtcagttgct gaaaggtgag tccgcgcagt tcttcctgac tgtaaccgag aaactggcag
     6721 agcgctttgt tggattgcag ccattgcccc tcggtgccca ctaatgccat gccgatggcg
     6781 gaatattcca tcgcgttccg aaagcgggtt tcgctttcgg aaatgtgttt gcgttccgca
     6841 cgaaaggcat acatcaccat ggtcatgatg ttggcgggca gcaggatcag caaaaacggt
     6901 agccacggca tatggctcat caggtatgta cgcggcgtag caagcaggga gggatccgcg
     6961 gccatcatca gtgacaccat catcaccgtg gtaaggaaga tcaaaaaggc ttccattcgt
     7021 ggcaggcgca cggcgctcca catcaacagc acaataatga aagtaaaagg ccacggcaga
     7081 tacagcatcg aaagccagct taacgtcagt gtgatggcta acgtgagcag cgactcaaaa
     7141 agcaaccgtg ggttgcgatg gcgcagcaga tagtgtggtt taaataacaa tcccagcggc
     7201 accagtgcca gtgcgccgat ggattctgac agtacccata tcaaaaatgc cctgagagga
     7261 tcgtctccgg gcgtcagcag gataaccaga acacccccta acagaggtgg aacaatggcg
     7321 ctgccgagtg ccagacgcag tcagtcagcc agattttgca atgggttata ccacggcagc
     7381 aatttacgca gtagcactgc cccgaccacg gcttcaacaa tattgatggt cgtccaggtc
     7441 atgttcagcg agctggtgga aaaaagcagg atggatgcgg cgatatttcc cagcgagcag
     7501 ctcagcgcaa tccccggcca catgcgcccg gcatggcgat aaaacgccac catcatgatg
     7561 gacgtcggga accacagtgg ggcgagttgg gtgccaaact gcgaaagctc aagcgagaaa
     7621 agggtaaaga taaacgagac taaacctaaa ctgaccaagt gaagcagcgg gtggggcagg
     7681 gcaattaata catgctgtga ttgtttgctc attaccgctt tatccgcaag accaggtcgc
     7741 cagtattcgt ttctgtgttt atgctagtac aaaagtttta cattttatat gcgaattgct
     7801 cataaagtga cgtaaaggcg gataatttgc gcaacggcgt ataacatttt ttaccttgta
     7861 taaaactgat caacgtaatt tgccaccggg aattcttcgt caacaattaa ctctgagcgc
     7921 gcggcaacgc tattcgactg gtatcaggcg gatgaaatcc ctataattgc cgcgtttggc
     7981 gcttcgtcgc ccccttccta acatccaggt taatcaggtc gctaaatata tgactgatca
     8041 gtctcatcag tgcgtcatta tcggtatcgc tggcgcatcg gcttccggca agagtcttat
     8101 tgccagtacc ctttatcgtg aattacgtga gcaagtcggt gatgaacaca tcggcgtaat
     8161 tcccgaagac tgctattaca aagatcaaag ccatctgtcg atggaagaac gcgttaagac
     8221 caactacgac catcccagcg cgatggatca cagcctgctg cttgagcatt tacaagcatt
     8281 gaaacgcggc tcggccattg acctgccggt ttacagctat gttgaacata cgcgtatgaa
     8341 agaaacggtg acagttgagc cgaagaaagt catcattctc gaaggcattt tgctgctgac
     8401 ggatgcgcgt ttgcgtgacg aacttaactt ctcaattttc gttgataccc cgctggatat
     8461 ctgcctgatg cgccgcatca agcgtgacgt taacgagcgt gggcgttcaa tggattcagt
     8521 gatggcgcaa tatcaaaaaa ccgtgcgccc gatgttcctg caattcattg agccttctaa
     8581 acaatatgcg gacattatcg tgccgcgcgg cgggaaaaac cgcatcgcga tcgatatatt
     8641 gaaagcgaaa ataagtcagt tctttgaata agcttgataa attgtgtacc gttcagtgat
     8701 aacctggtat gcccttgacg taattggcgt taagggagtg atgcgcgaaa ggagaaaatg
     8761 ccatgcgtct gtgtgaccga gatattgaag cctggcttga tgaaggccgt ttgtcgatca
     8821 acccacgtcc gccagtggag cgtattaacg gcgcgacggt ggatgtgcgc ctgggcaata
     8881 aatttcgtac cttccgtggt cacacggcag cgtttatcga tctgagcggt cccaaagatg
     8941 aagtgagcgc cgcgcttgac cgcgtgatga gcgatgaaat cgtgctcgac gagggcgagg
     9001 cgttttatct tcacccagga gagctggcgc tggcggtgac gctggagtcg gtgacgctgc
     9061 cagccgatct ggtgggctgg ctggacgggc gttcttcact ggcgcgtctg gggctgatgg
     9121 tgcacgtcac cgcgcaccgt atcgatccgg gctggtctgg ctgcattgtg ctggagtttt
     9181 acaactccgg taagctgccg ctggcgctgc gtccgggcat gttaattggt gcgctgagct
     9241 ttgagccgct ctccggcccg gcggcgcgac cttacaaccg ccgtgaagat gcgaaatatc
     9301 gcaaccagca gggcgcggta gccagccgaa tcgataaaga ctaattcagg cccattgagg
     9361 ataccatgag acgatttctg acgacgctga tgatactcct ggtcgtgctg gtggccgggt
     9421 tatctgcgtt agtgttgctg gtgaatccga atgatttccg cgactatatg gtcaagcaag
     9481 ttgctgcacg tagcggttat caattgcagc tcgacgggcc actgcgttgg cacgtctggc
     9541 cgcagcttag tatcctctcc gggcgaatgt ctctcaccgc ccagggcgca agccagccac
     9601 tggttcgcgc cgacaacatg cgtctggacg tggcgctttt accactactg agtcatcaac
     9661 tgagcgttaa gcaggtgatg ctaaaggggg cagtgatcca actgacgccg cagacggaag
     9721 ctgtgcgcag tgaagacgct ccggttgcac cgcgcgacaa taccttgccg gatctgtcag
     9781 acgatcgcgg atggtcgttt gatatatcca gtcttaaggt ggcggacagc gttctggtgt
     9841 tccagcatga agatgacgag caggtgacaa tccgcaatat ccgcctgcaa atggaacaag
     9901 atccccaaca tcgtggttca tttgagttct ccgggcgggt taatcgcgat cagcgcgatc
     9961 tcacgatatc ccttaacggt acggtggatg cttctgatta tccgcatgat ttaatggcgg
    10021 ctattgaaca aattaactgg cagttgcagg gtgccgattt accaaaacaa ggtattcagg
    10081 ggcaggggag tttccaggtc cagtggcagg agtcacataa acgcctttca tttaaccaaa
    10141 ttagtttgac cgccaatgac agtacgctga gcgggcaagc acaggtcacg ttgacagaga
    10201 aaccggaatg gcagctgaga ctgcaattcc cgcaactgaa tcttgacaac ctcatccccc
    10261 ttaatgaaac agcgaatggt gagaacggtg ccgcgcagca agggctgagc caatcaacgt
    10321 tgccgcgccc ggtcatttct tcgcgtattg atgaaccggc ctatcaggga ctgcaaggct
    10381 ttacggctga tattttgttg caggccagta acgtgcgctg gcgcggaatg aattttacag
    10441 atgttgccac gcaaatgacc aacaagtcgg gtttgctgga aattactcaa ctgcagggca
    10501 aacttaacgg tggacaggtt tcactgccgg gcacgttgga cgcgacatca ataaatccgc
    10561 ggataaactt ccagccacgg ctggaaaacg ttgagattgg caccattctg aaggcgttta
    10621 actatccgat ttcgttgacc ggaaagatgt cactggctgg tgatttctcc ggtgctgaca
    10681 tagatgccga cgcattccgc cacaactggc aaggacaggc acatgtcgaa atgaccgaca
    10741 cacgcatgga agggatgaac ttccagcaga tgattcagca agcggtagaa cgtaatggtg
    10801 gtgatgtgaa ggccgctgaa aacttcgata acgtgacgcg tcttgaccgc tttaccaccg
    10861 atttgacgtt gaaggatggc gtcgtgacgt taaacgacat gcaaggtcaa tcgcccatgc
    10921 tggcgctttc cggggctggt acgttgaatc tggcggagca aacctgcgac acccagtttg
    10981 atattcgggt cgtgggtggc tggaacggag aaagcaaact gattgatttc ctcaaagaaa
    11041 cgccagtacc gctgcgggtt tatggcaact ggcagcaact taattacagc ctgcaagtgg
    11101 atcagttact gcgtaaacat ctacaggatg aagcaaaacg ccgtcttaac gactgggccg
    11161 agcggaataa agattcccgc aatggcaaag atgtgaagaa gttgctggag aagatgtagg
    11221 ttaccaggtg acctgtcctg taggcctgat aagacgcgta aagcgtcgca tcaggcgttc
    11281 gtggcggcac cattatattc tgaatatgta agatcgaagg ctgttccgtt cacttgatgt
    11341 tcctcgctac tatggaacgc tcaagccggt gaacgtgcca gggtggctca atcgccacca
    11401 ccctggcaac ccaggctccc ggcggtaaat cgccgctggc gtggttccct catcttattc
    11461 ctttcggcta tcgggtacgg gcgcgaggta acatcactgt aaaacgcgcc ctcagcccac
    11521 atccatgtgg gctgccccgg cctacaggaa acgattcggc gatttacagc cggacccaca
    11581 cccccgctgt gatgtccatg taccacagac aacgcttcat tgctgaagtt ataaggcgtt
    11641 acgcagcatt gggtaaaatg gccgatacca tcacacctcg tactccatct cgccatcctt
    11701 acgcagcgga ataatctgca ccttctgcac gcgatggctt tctacctgca acgttttaag
    11761 caaataatcc cccacctgaa cttcttcgcc aggttttgga atacgctgca aatattccat
    11821 caatagcccg gcaatggtgt gatattcacg tttttcatcc agcggcagcg gcacatattg
    11881 caccagatct tccagcggca tatgaccatt cgccgtccag gaaccgtccg cattcttctg
    11941 aatatcatgg cgggcgtcga tctcttccac ttcgttcggt aagttaccgg caatggtttc
    12001 agtgacgtca cttaatgtca caatcccttc caccgagcca aactcatcca ccacaaaagc
    12061 aaagtgtgtg cgggcattac ggaactgctc cagggcaggt aacaacggca aggtttccgg
    12121 gaacaccagc ggctgacgaa tcaacacccg caggttgagc ggttcgccgc gcagtgactg
    12181 ttgtaacagg tcgataacgt gaacaacacc gagcaaatct tctgcgtcat cgccatcagt
    12241 aacgacaagc cgcgtatgct gatttcgctc cagcaactgg cggatctcgt cttccggcgc
    12301 gttgagatcg atatgctcaa tatcgtggcg cgacgtcata atgctgctga cggtacgctg
    12361 gttaagatta agtacccgct caatcatccg ccgttcctgc ggattaaaga tctgctggtt
    12421 accatgatcc accagcatgg acgcggtttc ggcgtccagt tccgcatctt ctttttgccc
    12481 gctaagcagg cgcattaccg cttcggtagt ccgctggcgc agtgtctggt tagcggaaag
    12541 aaaacgtcgc cggttaaaga tagccaactg attgaacgcc tcgatcatca ccgagaaacc
    12601 aatggcagcg tacaggtagc ctttcggaat gacgaagccg aaaccttccg ccaccaggct
    12661 aaagccaatc atcaacagga agcttaagca gagaataacg atcgtcggat gactgttaac
    12721 gaactgggtt aacggcttgc tggccatcaa catcaggctg atagcgataa ccacggcggc
    12781 catcatgacc agtaaatggt cgaccatccc gatggcggta atcaccgagt cgagtgagaa
    12841 gatagcgtcc agcaccacta tttgtgtcac cacgccccag aacttcgcac ctttgcgttg
    12901 cgtggggtta ttgctatctt tcccttccag ccgttcgttc agctccatcg tggctttgaa
    12961 caacaggaag aaaccaccaa acagcatgat taagtcgcgg gcgctaaagg taaacgagcg
    13021 gaagctgaac agcggttgag taagggtgac cagccaggag atagaggcca gcagcaggag
    13081 acgcatcacc atcgccagca tcaggccagt gatccgcgct cggtcgcgct gggcgggagg
    13141 gagcttctcg gcgaggatag cgataaagac taggttatcg atacccaaca ccagttcgat
    13201 aaccaccagc gtgacaagac ctgcccagat tgacggatcg gcaatccatt ccatatgtat
    13261 taaaacctgt acgactgcag tatgtgttat cacgatcatg gataagatta gtctaaaatg
    13321 caatgtatgg gtccaccaca tattgcaatt tttgttctct tttaacatat attccacggg
    13381 cgtcattagt gcgagcgcct tgtggggtct tttgctgtta tacagcacca gatattccgc
    13441 cagcttctgg ttaaacaacg ccagatcctc aaagagtaaa atttcattaa actcaataaa
    13501 ttgctctctt aatgtccggt taaaacgatc acagatagcg ttcatttttg gcgtgtaggg
    13561 ataggtccag atgtgtttga tggcggcttc ctgcagcgtt ttgtcaaagc ttcccaggaa
    13621 ctctttcccg ttatctgaga tgatctggct gataccgata ggaaacagcc gggctgcacg
    13681 gctgaaaaag tgattgacga tatcactgtt gagcgacggc acagccagcg ccagtgcgta
    13741 atcgctgtgt tcgtcgatca tggtaatgat atagcgacgc atttcaccca ttctgagctc
    13801 aatggcgtcc atcccaatga gctctccggt ctttaccgga cgatagtgct ttggcctgcg
    13861 gggtttggtg gttcgctttt tgacaaacag agccttaccc cgcgagctca gacgtactgg
    13921 tatcatccgc attttatcgt gtgcggtggc gatcattctg ccgatggtgg aaacactcgg
    13981 gcaggtcagg tggcgttgta cgcaccaggg cttcaggcga acaaaaatct gctctttacc
    14041 gaggttcggc agctctgtcc tgaggcgtcg aatctctttc agcacatcgg gatgccagtg
    14101 cttttttcgt cgcaccagag gtgccttact gtgcgggatt ggcccctcag gtcctccctt
    14161 gttcagtaac tgacgccacc agtaaggcgt gcggcaggat acgccaaagg cttcagacgc
    14221 tgcagaaata tcgtgtttat cccagaagtg cagagccttc attctcattg ttgctatatc
    14281 agccattact gaaagcctca acgcataatc ggctgtttgg tagtacttag gatagccaac
    14341 catcaggtgc tgcatttgca tagtgcaatc tccccttact tatccagagg caattgcaat
    14401 atctctgtga acttatacag gcaataacga cgcggacatc gctctgccgt gggtggatat
    14461 atttccagcg gattaagcga accgataaga attatgttcc ggaaaagaac agtgaaaaaa
    14521 taacaccatg gcggcgacca tggaataagc tttccgatat cagaacttta tccgctgcgt
    14581 ttcagcgagg gtggcaaaag ggcttactcg cgcggttcgt cacggtgagc gctgatgcgc
    14641 agagcagaag cgaagcgcga agcccgctta caaaaccggc ataattcata ttatgaagtg
    14701 agtgcagaat agggtggatc gcagttcaca gccgcggatt caggctaata tgtcgtcata
    14761 tccggctgtt tatactgaag tatattgcct ggcgaaattt attatctata tattcttttc
    14821 gggcatacgc gcggcgggtc cggcgtccaa caggtcccga tagacgggca tctcagccac
    14881 atcgtgcgga gcaaaggaac gagccgctga ataacctgaa cgagaataag attattctca
    14941 gttttaactg ttggtaaaag ggagttgtgt gtattctttt acgtgacagt gaatttaaga
    15001 atttggcggg catatgctcg ggcagtatcc gtgtattatc agtttcaatg aatttgtgac
    15061 atgtacaatt ttgcaaaacc gcgtgttttg caaccaaaca ggtgaagatg aatatggcga
    15121 atttgaaagc ggttattccg gtcgcaggac taggcatgca tatgctgccg gccacaaagg
    15181 caattccaaa ggagatgctg ccgatcgttg ataagccaat gattcagtac atcgttgacg
    15241 aaatcgtcgc cgccgggatc aaagagattg ttctggtgac gcactcctcg aagaatgcgg
    15301 tggaaaacca ctttgatacc tcctacgagc tggaagccct gcttgagcag cgcgtgaagc
    15361 gccagctgct ggcggaagtc caggccattt gcccgccggg tgtaaccatc atgaacgtgc
    15421 gccaggcaca gccgctgggt ctgggccact ccatcctatg cgcccgcccg gtagtcgggg
    15481 acaacccgtt tgtggtggtg ctgccggata tcattctcga tggcggcact gcggacccgc
    15541 tgcgctataa cctcgctgcc atggtcgccc gttttaacga gaccggccgc agtcaggtgc
    15601 tggcgaaacg catgccgggc gatctctccg aatactccgt tatccagacc aaagagccca
    15661 tggtggcgga agggcaggtg gcgcgtatcg ttgagttcat tgaaaaaccg gacgaaccac
    15721 agaccctgga ctccgacctg atggcggtgg gccgctacgt gttgtcggca gatatctggg
    15781 ctgaacttga acgaacccag ccgggcgcct ggggccgtat ccagctaacc gacgccattg
    15841 ccgaactggc gaagaagcag tccgttgacg cgatgctgat gaccggcgaa agctatgact
    15901 gcggtaaaaa aatgggctac atgcaggcct tcgtcaccta cgggatgcgt aacctgaaag
    15961 aaggtaccaa attccgcgaa agcattaaaa aattgctggc ctgagcgccg gcgtaagagt
    16021 gagcggcagg gcaggggtat tttgccacgc cgctcataac gttatgcaga aattattcat
    16081 aacgtgaatg ttataacgat tgtaaagaaa cgtatggctt cggagacttc tggtagtttg
    16141 cctctggcct ctgtggtgca atggtcagag tagcggcaat aatgctttat gaatagtcac
    16201 ttagtgcgac aattcctaaa gtgcagggaa agcggtctga ggtgtactgg caatagcgga
    16261 cactaccatt tgttcttttt ttaagcagtc atctgatgat atttttccct gaaggctgcc
    16321 ggggagatat tccccagacg agagtgacga cgctgacgat tgtagaaaat ctcaatgtat
    16381 tcccgtatta ctgagatggc ttcatcccgg ttattaaaac gatagtggct caggctctca
    16441 tttttcagcg ttccccagaa gctttccatc ggagcgttgt cgtaacagtt acctttacgc
    16501 gacattgatg ttttcagacc aaactgctcc tgtatgaccc ggtaatcgta tgcgcagtac
    16561 tgtgaacctc gatcagagtg gtggattagc ccggcaggtg ggcgctggct cctgagcgcc
    16621 ataaacaggg ctttacctgt cagctctttt gtcatgcgct ctcccatggc gtagccgaca
    16681 atttcgcacg tataaacatc tttgatgcca gcgaggtaca accatccctc ctgtgtggca
    16741 acatacgtca ggtccgccac ccagacctga tttggtgctg taggagcgaa cgtctggttc
    16801 agcagatttg gcgcaactgg cagattgtgg ttcgagttcg tagtcgctct gaacttgcgt
    16861 ttctgcttac agcgtagcct tagctcctta cgaagacgtg ccagtcggtc acgaccaacg
    16921 atgatgccat tctctgccag ctccgtctgg agccgccggg ttccatatgt ttcgcgagtg
    16981 cggatatgtg ccaccttaat ctccagtttt agccgctcat cactttgttt tctgtctgag
    17041 ggttcatgct gtacccagtt gtaataaccg ctcctggata caccaaatac ctgacacatc
    17101 gcttcaatgg gaaattgttg tcgccattgt tcgattaacg cgtatttt
//
LOCUS       DAPHJE010000095        16577 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPHJE010000095 DAPHJE010000000
VERSION     DAPHJE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.096"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(85..237)
                     /locus_tag="RH222_003253"
     CDS             complement(85..237)
                     /locus_tag="RH222_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV3798231.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(502..1236)
                     /gene="cysH"
                     /locus_tag="RH222_003254"
     CDS             complement(502..1236)
                     /gene="cysH"
                     /locus_tag="RH222_003254"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3798232.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMLEEETRFFGLKRECGLHEG"
     gene            complement(1310..3022)
                     /gene="cysI"
                     /locus_tag="RH222_003255"
     CDS             complement(1310..3022)
                     /gene="cysI"
                     /locus_tag="RH222_003255"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV3798233.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3022..4821)
                     /gene="cysJ"
                     /locus_tag="RH222_003256"
     CDS             complement(3022..4821)
                     /gene="cysJ"
                     /locus_tag="RH222_003256"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV3798234.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5140..5502
                     /gene="queD"
                     /locus_tag="RH222_003257"
     CDS             5140..5502
                     /gene="queD"
                     /locus_tag="RH222_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV3798235.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5580..6851
                     /locus_tag="RH222_003258"
     CDS             5580..6851
                     /locus_tag="RH222_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3798236.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6842..7102
                     /locus_tag="RH222_003259"
     CDS             6842..7102
                     /locus_tag="RH222_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV3798237.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7119..7694
                     /locus_tag="RH222_003260"
     CDS             7119..7694
                     /locus_tag="RH222_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV3798238.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7842..8702)
                     /locus_tag="RH222_003261"
     CDS             complement(7842..8702)
                     /locus_tag="RH222_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV3798239.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLIIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPKVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8699..9478)
                     /locus_tag="RH222_003262"
     CDS             complement(8699..9478)
                     /locus_tag="RH222_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV3798240.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9456..10865)
                     /locus_tag="RH222_003263"
     CDS             complement(9456..10865)
                     /locus_tag="RH222_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3798241.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10887..12341)
                     /locus_tag="RH222_003264"
     CDS             complement(10887..12341)
                     /locus_tag="RH222_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV3798242.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12411..13196)
                     /locus_tag="RH222_003265"
     CDS             complement(12411..13196)
                     /locus_tag="RH222_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3798243.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAASCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13515..14792
                     /locus_tag="RH222_003266"
     CDS             13515..14792
                     /locus_tag="RH222_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3798244.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14819..16297
                     /locus_tag="RH222_003267"
     CDS             14819..16297
                     /locus_tag="RH222_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3798245.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNYFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3952 a         4354 c         4175 g         4096 t
ORIGIN      
        1 cataaaaaat ataaatttta aaaaagggtg caggagacca aaccatcaca attcagtacg
       61 cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa ctgccttaaa
      121 ctccatacca cgttctctga tactcaactc acacaacgag tcacctacca tcagcgtgaa
      181 tcccagtact gtacaacaca gtacgatgat tgccacaagg gcatattttg tcagcatgtc
      241 ttgcctccct gcaaagaaga gactaacatt cgaattgttt gggttcgaac gtaagcctca
      301 ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa tgcttaacgc
      361 cagacagcct caagcacccg acgccattct atacctgata attcttccag cggcgttcac
      421 gtttaaaatc aggaatactg ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc
      481 gtttaattta tccggcaata tttacccttc gtgcaatcca cattcccttt ttaagccaaa
      541 gaaacgtgtt tcttcttcca acatgccagg ttcccattta cgggttgtat gggtatcacc
      601 gaccgataaa tatccttcat cccataatgg gtgatatttc aggccatgtt tttgcaggta
      661 ctgataaata gttcggttat cccagtcgat aatcggcagc actttaaata cgccacgctg
      721 aattgccagc accggtaaat tggcgcggct gccggattgt tcgcggcgca ggccagcaaa
      781 ccacgtttgc gcattcagtt ctttcagcgc ccggttcatc ggttcgactt tgttgatgtc
      841 attgtacttt tcaatgcctt caacgccctg ttcccacagt ttgccgtagc gtgcttcctg
      901 ccaggcggcg ctttcggtag cgcggtacac tttcaggttg agcttgagtt tgtccgttaa
      961 ctcgtcaata aagcggtagg tttccgggaa cagatagccg gtgtcggtga ggatcaccgg
     1021 aatatccggg tgaatctgat tcaccagatg caggctcacc gccgcctgaa tgccaaagct
     1081 ggaagaaagt acatattcac cgggcagatt atccagcgcc caggcaacgc ggccttcagc
     1141 gtccagtttt tccagttcgg cgttagtttc tgccagcgcc agaatgcgat ctaccttcgg
     1201 cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt ttgccttgcc
     1261 tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt taatcccaca
     1321 aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga
     1381 agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca
     1441 ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt
     1501 tgccgccaag atgcagattg tagcgacccg gtgctttacc caccaggccc acttccgcca
     1561 gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc
     1621 tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct
     1681 ccgcttccgc catcgccagc gggcaagtcg ggaatgacac gcaggccatc gagttttcac
     1741 gctgcggcgt gacggcattc attaaaccgc tctctttggc gatcttctcg atcttcgctt
     1801 tctcgctttc cggtacaccg gcgatgatca gattctggtt cgccgtaatg cggaaatcgc
     1861 ctttgtggat cttcgcgatc tccagcaggc cggttttcag cggacgcccc ggataatcaa
     1921 ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc
     1981 agccaatacg atcgcctcgt ccggtgaact catatggacg gatcggttca aatttgatcc
     2041 ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc agcgtgtatt
     2101 tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg
     2161 cttcggccac cgccagcgta tgctccagcg gcagatagcc aaactcgctc gccgtgcggg
     2221 cgtaggtttt cttgttgccg tgttcgatag aaagcccacc gcccaccagc aggttgaagc
     2281 ctaccagctt gccgttttcg gcgattgcca cgaagttcat gtcgttggca tgcagatcga
     2341 tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg taggtctgac
     2401 caaggatcgg ttcttcatca gtagtggcga ctttttcctg gtcgagccag atctccgcat
     2461 acgcgcgggt acgaggcaac agatgctcag aaatcttctt cgcccactcg tacgcttccg
     2521 cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttag
     2581 ctgtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct
     2641 ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg ctgccataga
     2701 tggtgttttc accggcaaat ttgtcgatcg cctgccactg tttagtggta atcaccccac
     2761 ccggcagacg acagcgaagc agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt
     2821 cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt
     2881 tatcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca
     2941 ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag
     3001 gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag
     3061 ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc
     3121 cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg
     3181 ggcaccatca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt tgtcttgtac
     3241 gtaaactttt tctttttggt cgcgcgacca ggcaagatcg atacgtgtca gcacgccatc
     3301 tttgacgtag cgctgccact ccacctggta caggaagtct tccgtaaagt gcggattacc
     3361 aaagaacagc cagtttttac ctggcgcttc gtcggcggcg cgttgctgca taaaggcgcg
     3421 gaacggcgca ataccggtgc ctgggccaat cataatcacc ggggtttctg gattggctgg
     3481 taggcggaag ttatcgttat gttcgataaa tacgcggact tcgccctctt cttccacgcg
     3541 gtcagcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt cgtaacgcac
     3601 cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg caatggaata
     3661 gagacgcggc gtcagcgggc gcagcagatt aattagcgct tcggcatcaa gctgtgccgg
     3721 ggagaaacgc accatgtcga caatcggcgt cgtcgcggcg taatgctgta acttcgcttt
     3781 atcgcccacc agcggcagca gtgtttcgct gcgggtaagc gtggcgtaat tctcaacaat
     3841 gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt
     3901 tttgccctcg acggtgacag gttcatcgcc tttcagccac agcagttcga caagttcttt
     3961 caccagtgcc ggatcgttct gataccagac gcccagcgcg tcacccggct ggtaacgcag
     4021 gcccgagtca cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt tacgcccggt
     4081 aattttctgg ttaacagaga ggctagccac cagcggcgcg tctttgctgt acgggctggt
     4141 gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg
     4201 cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac
     4261 atcggcatcg acacggtcga gcaggcgttc accacccagt tccgccagct tgctgtcaaa
     4321 atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa acacggcaaa
     4381 tgcggtgttt tccagcttcg gcgctttttt ggagaacagg aacttatgca gcgcgacggc
     4441 ttcttccggc ggttcccctt ctccttgcgt tgacgtcacc acgataagca gtttttcgct
     4501 ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgccgc
     4561 taataaatca tcacgtaatg cttcagcaac acggcgcgca ttgccggttt gcgaggcgga
     4621 gataatagtt atacccggca tttctgcggc gggagctggc gtcgctgcaa gcgcagcagg
     4681 ctgctgattg agcacgcccc agaaatagcc agaaacccag gcaagctggg tgggagttaa
     4741 atcggtcgtg gccgcctgaa ggcgtaccag ttgctccggg ttcaacggaa gcaacgcgga
     4801 aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc
     4861 ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg gtgtggatta
     4921 aagacgggat agcgataatt aataaccaaa tcgactaacc tgttttagca atagtcttta
     4981 acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa tcattaagat
     5041 aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg
     5101 tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac gttatttaaa
     5161 gatttcacct tcgaagccgc tcaccgctta ccacacgtcc cggaagggca taaatgtggt
     5221 cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat
     5281 acgggctgga ttatcgattt cgctgaactg aaagcagcgt ttaagccaac ttatgagcgc
     5341 ctcgatcacc attatctcaa tgatattcca ggcctggaaa acccgaccag cgaagtttta
     5401 gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta
     5461 aaagaaacct gcaccgctgg ttgtatctat cgcggcgaat gataagagtg tgtcggcggt
     5521 caatttccct taagtaacgc tatgttaggg tgttgtgttc tggatatctg gggcatgaca
     5581 tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc gcttgcgcgt
     5641 tacgctgcgc gcgagcgggt ttatccgttt tgttactgga acgcgctgaa atccccggca
     5701 gcaaaaatct ttccggcggg cggttatata cccatgcact cgcggaactc ctccctcagt
     5761 ttcatctgac cgcgcctctt gaacgatgca tcactcacga aagcctttcc ctgttaacgc
     5821 cggatggcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac
     5881 gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc
     5941 ctggtgcgac ggtggatgca ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg
     6001 gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg
     6061 ctgagcgtca cgggttagtg actcgtcctg ctggcgaagc gatggcgttg gggatcaaag
     6121 aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag aataacgaag
     6181 gcgcagcgtt gctgttcagc ggcgggatct gtgatgactt acccggcggc gcatttcttt
     6241 atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa
     6301 gccgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca gtgcgcccgc
     6361 ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc
     6421 acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca
     6481 gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact ggcgcgcagg
     6541 cggcggcaca aacgctgata agcgcctgcc agcaccgcga gccgcaaaat ctgtttccgc
     6601 tttatcatca caacgtagag cgcagcctgc tgtgggatgt tctacagcgt tatcagcatg
     6661 ttccggcgct tttgcaacgc ccgggatggt accgtacgtg gcctgcgtta atgcaggata
     6721 tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc cagttattct
     6781 ggcatcattt acgtcgtcac ggcctgtggc atctggcggg cgatgttatc aggagtctgc
     6841 gatgtctgta gcccgtaatc tctggcgcgt tgctgatgcg ccgcacattg ttccggctga
     6901 ctccgttgag cgccagacgg cagaacggtt gattagcgcc tgtccggcag gtcttttttc
     6961 gctcacaccg gaaggtgact tacgtattga ctatcgcagt tgcctggagt gtggcacctg
     7021 ccgtttgctg tgcgacgaat caacactaca acagtggcgc tatccgcctt ccggattcgg
     7081 catcacctac cgctttggat aaataataag gataatttat gcccctctta cacctgctcc
     7141 gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg gcaatcgatt
     7201 ctgaatgcca atttatttcc gtgttgtacg gcaatatctg caccatcagt aatatcgtta
     7261 aaaagattaa gaacgccggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt
     7321 caaataaaga agtcgttatt cagtttttga aactggtgac cgaggcggac ggcattatca
     7381 gtaccaaagc ctcaatgttg aaagcggcaa gagcggaagg ttttttctgt attcatcgcc
     7441 tgtttattgt tgattcaatt tcgtttcaca acattgataa gcaagttgcg caatcgaatc
     7501 cggattgtat tgagatcctg ccaggctgta tgcccaaagt gctgggctgg gtgacagaga
     7561 aaatccgcca accgctgatt gccggtgggc tggtgtgcga tgaagaagat gcgcgtaatg
     7621 cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggggtctgg acgttagcga
     7681 aaaaattact ttgacgggat aatcgtaacc aattgaattt ggtttgattt gtaggccgca
     7741 cgccacatcc gacattcagc gcctgatgcg acgcttgacg cgtcttatca ggcctacaag
     7801 tccccaggcc agaaccgtag gcaggatcat ccggcaacac gttattggca atctgcgtga
     7861 atgtttgtca ccagtgcctc cagcaccact ttccagtcat caaccacgcc aacatctgcc
     7921 tgtgaaaata ccgcagcgct ggcgtcatga ttaatcgcca ccacaaactt gctattgcgt
     7981 acgcccgcca ttaacgctgc tgcgccagaa gcgccaacaa caatacacac cttaggtgcc
     8041 agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt catcactcgc
     8101 gcccgactgt agcccacttc ggctcccagc ttttctgcca gcatagcgat ttcctggtta
     8161 tctgcctctc cgccctgccc caccaccagc acccgtctgg cctcagctaa tgggtcgcga
     8221 gtaacatttt tcagatcctc tgtgctaacc agccagtccg gaagcgcacc cgggacaatg
     8281 atcagttgtt gcattccaga gggcaacgtg gcgtttttcg ctgctcccgc ctgacgcgcc
     8341 agggaaaggc atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc atttccccag
     8401 tgggatttgc gcacgcttac ggttggaata tctaacgaag tcacctggca gatgcttgca
     8461 ccatgtaaac gccaggccag ccgggtcgac agttcatcgc caaacgttcc cggcggaaag
     8521 agaacaacgt ccgcaggcgt tcgctgccac tgttccacca acgcatccag cacctgttcc
     8581 gccactacag gctgaggttc aatctgccag tgcgccaggg tacaaccgct gaaatcctgc
     8641 gccgccagcc agcttgctat tgcggcattc tcctgattta ttgtcacaat tgcgatattc
     8701 atggctgcat cctttggcgt aaatagtccc gccatagctt ttgagctttt tctgctaccg
     8761 tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt tgcagacact
     8821 gcatcgctgg catttcggca gcaacagttt tacggataat ttctgctttc cccgcagcca
     8881 ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc acggcaatca
     8941 ccgcaggcaa gcgaacccgg caacaacgca acccatgttc agtacgctgt tcgagggtga
     9001 taaacagcgc gtcgagagtg aaacgctcca cctgggtgaa gcagggccag cccagcattt
     9061 ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag ccggtaatta
     9121 tcagatccag cggattctga tgctgccatt cggcaatatg gcgagcgaca aattccggcg
     9181 caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca agagccatga
     9241 gatagcgcag ccagtgcaat gcccgttcat cccccatgct caacgcggtt aaagacatcg
     9301 gcgtaccgtt ttttctctgc gccaacagca gcgcggcagc ggcctgttca tcagcaccga
     9361 gtaaacttcg cagtagcgaa atatccggtc cgcttttacc ctgagccgcc gcctgccact
     9421 ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga atgttcattc
     9481 gcacctccta catttcccgc cgccaccagc gggagggctt tagtttctgg tgcccatagc
     9541 caggtcacaa caaaaccaac caacaacact gtcgccagga gcaataaggt gacttgcatt
     9601 ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcgcc aagtcgactc
     9661 atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact ttccgcaggc
     9721 aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac aaaaagcagc
     9781 agcgttaatg aactgccgga aggcaaacag gccatgacta ccagcgttgc cgccagcagc
     9841 aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac taatcccagc
     9901 agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc atcttccaga
     9961 ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg gattacgagg
    10021 cagacaaaga agacgctgtt aaacgccgtg cgccgccagt aacgcgaaga gaacaaggtt
    10081 ttgatgtgtt tatgggtcgc cgttaccact tcatcgccca gtaaaacatg gggaccaaaa
    10141 tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcgcagtag ccagcgtggt
    10201 gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc tgccgatgcc
    10261 agtagccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc aatacttgcc
    10321 agcacatagc ctacggtcca caccacgctg aatgcgccca gcaaaatacc gcgatggcgg
    10381 cgcggggaaa attcagccag caaggtgtga cctactgaat aatcgcctcc cagaccaatg
    10441 ccaatcaaaa tgcgcagtcc aataagatgc tctggcgtgg tggcaaaaaa ttgtaaaaac
    10501 gaagcaagcg taatcagcaa aaagctgaag gtgaagattt tttgccgacc aatatggtcg
    10561 gagatccacc caagaaccag gctgccaagg aacaaaccaa ggagcgccga gccgccgatc
    10621 attcccgcca taaacggcgt cagttgcatg gcgggcgtaa gctgaataat ggcgtaacca
    10681 atgacgccga gaacataacc gtcggtcagg tgtgcgccga aagtgagcgc agcaatgcgg
    10741 cagtgaaaac ggttaagcgg taaatcatcc attcgcaccg gtgaagtgtt catctttacc
    10801 ccttcattgt ccggtaataa gaacggcttc tcgcctgaga agccgaaaag gcaacgcccc
    10861 gtcatcgcgg aacgctgcct gacacattat ttttcaatcg gatagatagt acccgtattc
    10921 ataatgccat taggatcgaa ctgctttttc agcccttcca gcaacgccca cgcgctgccg
    10981 tgttccagtt tgctccagtg aacgcgatgt ttaccgatac cgtggtggtg caccatcgaa
    11041 ccgccgaggc gaatggtttc ttcacagatg atcttgttga gcggattgtg gtacttgtcg
    11101 atttcctctt ccggcttaca gtcaacgacg ttgtaatcgt agacgaagta catgttggtg
    11161 ccgttctgat agctatgaga ggaatgaccg cccagcatgg tgatgtcgtc ggcgtgcggg
    11221 aactcagtac gaatacggtt aataacgctt tcgtagattt cgtggatgca gctccagcag
    11281 ccggacactt cagtggtaaa gcccatgttg ccggttttga ggatctgcac acgttcggca
    11341 gccactttat ccggtcccca gttcaggttg ttaaaccagg tttcgatcag tttgctgtcc
    11401 acgcgctggc actgcgggta gcgggcaacg atttccgcaa tcccttcgcc cgtcgccttc
    11461 gcaatgcgag gattgccttc agccataaag atcagcacac atttaccgtc ggcaaaatgg
    11521 gtgaagtgtt gggtgccatc ttcagcgtca tacaaacgag cgatcgacgg acgataccct
    11581 tccaccatga cttcacgcag gatgttgaag ccggttttca tgtcttccag gatatagccg
    11641 tagaagaggt tgttttccgg ggtgaattta aagattttca ctgttacttc agtgatatag
    11701 caaagtgcac cttcgttacc gatgatgatg tggcgaatgt ccgggccagc cgcgcggcgt
    11761 ggcacgtttt taatgcgtgt gacggtgcca tcagccaata ctgcttccag accaacgacc
    11821 atatcttcga ttgcgccgta gagtgtggag aactgcccga tactgcgggt tgctaccagg
    11881 ccgcccatct gcgccagcgg ctttgactgc ggagaatgcc ccgtggtgta acctttttca
    11941 cgcaacgcat tttccagcac ctccagcgga acgccacatt gcgccgtcgc ctgcatattc
    12001 tcaatatcaa tattaatgat ttgattcatg gcggagccgt cgagcaccac cgagttttcc
    12061 acaacggttt ccagcccacc ttcagtggcg gaagcgccgg tacgcggcac accgttaatt
    12121 ttgtgcgcat tcataaaatt cagcacacgg gagacttgtt ctgtggaacc gagttttacg
    12181 accgctgccg gaatcggcaa agtataaata ccatgaatat ccggaaattt acgaaaacgg
    12241 tcaatactgt tcttctttaa tactgtttca tcggtaatta cgcgatctgc accaacaatt
    12301 tcctttagtt ggtcgacaat cgctgcgcga gataaagaca tagtaattcc ttcctgataa
    12361 taaaaatgga ataatcatta ataagagaaa acagaaacca ccgttatggg ttagcgcact
    12421 aaataaccgc catcaaccac taataaatgc ccgttgacat aattcgatgc cggacttgcg
    12481 aggaatacgg ctgcgcccat taaatcctga gtatcgcccc aacggtttgc cggaatatga
    12541 tcaagaacgc gctgattggt ttctggatta ctgcgtgtcg ccagcgtaat atctgttgca
    12601 taatagccag gggcgatacc atttacctga atattatatt gacctagttc atcacaataa
    12661 gctttggtga acccggcaag agcatgttta gtggcagaat atgcaggtga ccattgtcca
    12721 cctaagtaag agaacaatga acagatatta ataattttgc cgcttttctg cgggatcata
    12781 atttttgcag cttcatagct taactcgaat gcggcggtca ggttcacatc aatcatcgga
    12841 tcccagtcgg cacgaccgaa gtccagcacc ttattcagct tacaaatacc ggcattgtta
    12901 accagaatat caactgtacc gaaacgctca cagctagcag cgataatctt ctgcggcgcg
    12961 ccttctgcgg tgatatccac ctgcatgaag tccacctcaa caccctgttt ttcaatcatt
    13021 tcctttgttt cgccgttatc tttgacgaaa ctaggaataa agatatttgc gccagctttg
    13081 gccaacgcca tggcaaatgc ctggcctaaa ccgctattcc caccggtaac aattgcggtt
    13141 ttacctttca gggagaaaaa atccattgag aacgcattga gagattcgat tgacatagtt
    13201 agctccaatt tccgtaaggc aaaaaaaaag aaaggtaact tcccccgcga gagggaagtt
    13261 acctttctca ttatctctgg taactgtaga taatttttag cataacggca taactgctaa
    13321 tgtgatcgta atcacagtgt gatattcgta ttgtcaaata gttttaaaaa gcagaagcgt
    13381 gattactctc acacaacacc tctgctttcc atgttatttc ttattgcaat tactagagac
    13441 ctgagaaaag taatccacct gaaagggcgg cttacttttc tctttttttt gtctattaca
    13501 aatgggaata cgatatgcaa cacaactcat atcgccgttg gataaccctc gcgataatta
    13561 gttttagcgg cggcgttagt ttcgacctgg cttatttacg ttatatttat caaattccca
    13621 tggcgaaatt tatgggattc agcaataccg agataggttt aataatgagt acctttggta
    13681 ttgcggctat tattctttat gcccccagcg gcgttattgc cgataaattt tcacaccgca
    13741 aaatgattac ttccgcgatg atcattaccg gattactggg tctgttaatg gcaacgtatc
    13801 caccgctctg ggtaatgctc tgtattcagg tcgcctttgc gataacaacg attttaatgc
    13861 tgtggtcggt gtcgattaaa gccgcctcgt tgctgggcga tcatagcgag caagggaaaa
    13921 ttatgggctg gatggaaggg ctgcgcggcg tcggtgtaat gtcgctggcg gtgtttacca
    13981 tgtgggtctt ttctcgcttt gcaccggatg acagcaccag cctgaaaacg gtcattatca
    14041 tctatagtgt ggtttacatc ttgttgggga ttctgtgctg gttttttgtt agcgataaca
    14101 acaacctgcg cagtgccaat aacgaagaaa aacagtcatt ccagcttagc gacatcctgg
    14161 ccgttttgcg catcagcacc acctggtatt gcagcatggt gatttttggt gtcttcacca
    14221 tctacgccat tctgagttac tccaccaact atctgaccga aatgtacggc atgtcgctgg
    14281 tggcggcgag ctacatgggg attgtgatca acaaaatctt ccgcgcgctg tgcggcccac
    14341 ttggcggcat tatcaccacc tacagtaaag tgaaatcccc tacccgcgtg attcaaatcc
    14401 tttccatcat cggtttgctg gcgttgacag ccctgctcgt cacgaactct aacccgcaat
    14461 cggtcgcgat ggggattggc ctgattttac tgctgggatt cacctgttac gcctcacgcg
    14521 ggctgtactg ggcctgcccc ggcgaagcga gaacaccgtc ttacattatg ggcaccacgg
    14581 taggtatttg ttcggtgatt ggattcctgc cggatgtctt cgtttacccg attatcggcc
    14641 actggcaaga caccctgccc gctgcagaag cctaccgcaa tatgtggctg atgggcatgg
    14701 cggcgctcgg catggtgatt gtctttacct ttttgctgtt ccaaaaaatt cgtactgctg
    14761 atagcgcccc cgcaatggct agcagcaagt aagccaggaa aattacagga gaaaaatcat
    14821 gtcgaaaaaa tacatcatag ggattgatgg cggaagtcag agcacaaaag tggtgatgta
    14881 cgatctggaa ggtaatgtgg tttgcgaagg taaaggctta ttacagccga tgcacacgcc
    14941 agatgccgat actgcagaac atcctgacga cgatttatgg gcatcattat gttttgccgg
    15001 tcacgatttg atgagtcagt ttgccgggaa taaagaagat attgtcggta ttggtctggg
    15061 atccatccgt tgctgccgtg cgttattgaa agccgatggc acgccagctg cgccgttgat
    15121 tagctggcag gatgcacgcg ttacacgccc ttacgaacat acgaatcctg acgtggcgta
    15181 tgtcacctct ttttcgggtt atctgacgca tcgcttaacc ggcgagttta aagacaatat
    15241 cgccaactat tttggtcagt ggccggtgga ttataagagc tgggcatgga gcgaagatgc
    15301 tgcggtaatg gataagttta atatcccccg tcatatgctg tttgatgtgc aaatgcctgg
    15361 caccgtcctc ggacatatca caccacaagc cgcactggcg acacatttcc cggcaggact
    15421 gccggttgtt tgtaccacca gtgataaacc ggtagaagct ctgggggccg gattactgga
    15481 tgatgaaact gcggtaattt ctcttggcac ttacatcgca ttgatgatga acggcaaagc
    15541 actgccgaaa gatccggtgg cgtactggcc gattatgtct tctattccgc aaacattgct
    15601 gtatgaaggt tacggtattc gcaaaggtat gtggacggtg agctggctac gcgacatgtt
    15661 aggcgagtcg ttaattcagg atgccaaagc acaggatctt tcaccggaag atttgctcaa
    15721 caaaaaagct tcttgtgtgc cgcctggctg taatggtctg atgacggtgc tggactggct
    15781 gaccaacccg tgggaaccct acaaacgtgg gattatgatc ggctttgatt ccagcatgga
    15841 ttacgcatgg atatatcgtt cgatactgga aagcgtggcg ctgacgctga agaacaatta
    15901 cgacaatatg tgtaatgaaa tgaattactt tgcaaagcat gtgatcatta ctggcggcgg
    15961 ttcgaacagc gatctgttta tgcagatttt tgccgacgtg ttcaaccttc cggcacgacg
    16021 taacgccatt aacggttgtg caagcctggg ggcagcgatt aatacagcgg taggtctggg
    16081 gctatacccg gattacgcaa cggctgtcga taaaatggtt cgcgtgaaag atatctttat
    16141 gcctgttgag agcaatgcca aacgctacga cgcgatgaat aaaggcattt tcaaagattt
    16201 aaccaaacac actgatgtga ttctgaaaaa atcgtatgaa gtgatgcatg gggaattggg
    16261 gaatgcggat tcgatccaga gctggtcgaa tgcgtgaatg aggtaggcca ggcggaaaac
    16321 tgcccggcct gaacatacct aaatagttat ccccgctgac gcggggaaca taagttcgac
    16381 aacttaaaaa atgataacaa gtccggttta tccccgctga tgctgggaac ataataaaaa
    16441 ctttaactag ttcggtttat ccctgttttt acaaggaata ttaactacac tcgatgtctt
    16501 tagaataaaa gacaatacga atcgcactct atcaattttc acttaaagta tgaactggat
    16561 atctttattc ataagaa
//
LOCUS       DAPHJE010000096        16484 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPHJE010000096 DAPHJE010000000
VERSION     DAPHJE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.097"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="RH222_003268"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="RH222_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV3798246.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2791)
                     /gene="hemX"
                     /locus_tag="RH222_003269"
     CDS             complement(1574..2791)
                     /gene="hemX"
                     /locus_tag="RH222_003269"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV3798247.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQADTSAAAPQGE"
     gene            complement(2813..3553)
                     /gene="hemD"
                     /locus_tag="RH222_003270"
     CDS             complement(2813..3553)
                     /gene="hemD"
                     /locus_tag="RH222_003270"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV3798248.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3550..4491)
                     /gene="hemC"
                     /locus_tag="RH222_003271"
     CDS             complement(3550..4491)
                     /gene="hemC"
                     /locus_tag="RH222_003271"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV3798249.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4878..7424
                     /gene="cyaA"
                     /locus_tag="RH222_003272"
     CDS             4878..7424
                     /gene="cyaA"
                     /locus_tag="RH222_003272"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV3798250.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7464..7784)
                     /gene="cyaY"
                     /locus_tag="RH222_003273"
     CDS             complement(7464..7784)
                     /gene="cyaY"
                     /locus_tag="RH222_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV3798251.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7946..8088)
                     /locus_tag="RH222_003274"
                     /pseudo
     CDS             complement(7946..8088)
                     /locus_tag="RH222_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8247..8450
                     /locus_tag="RH222_003275"
     CDS             8247..8450
                     /locus_tag="RH222_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3798252.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8487..9311
                     /gene="dapF"
                     /locus_tag="RH222_003276"
     CDS             8487..9311
                     /gene="dapF"
                     /locus_tag="RH222_003276"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV3798253.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9308..10015
                     /locus_tag="RH222_003277"
     CDS             9308..10015
                     /locus_tag="RH222_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV3798254.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMVLLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10012..10908
                     /gene="xerC"
                     /locus_tag="RH222_003278"
     CDS             10012..10908
                     /gene="xerC"
                     /locus_tag="RH222_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV3798255.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10908..11624
                     /gene="yigB"
                     /locus_tag="RH222_003279"
     CDS             10908..11624
                     /gene="yigB"
                     /locus_tag="RH222_003279"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV3798256.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11708..13870
                     /gene="uvrD"
                     /locus_tag="RH222_003280"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11708..13870
                     /gene="uvrD"
                     /locus_tag="RH222_003280"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV3798257.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(14017..14781)
                     /locus_tag="RH222_003281"
     CDS             complement(14017..14781)
                     /locus_tag="RH222_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV3798258.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLI
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFSCYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15151..16101
                     /gene="corA"
                     /locus_tag="RH222_003282"
     CDS             15151..16101
                     /gene="corA"
                     /locus_tag="RH222_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV3798259.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16144..>16484)
                     /locus_tag="RH222_003283"
     CDS             complement(16144..>16484)
                     /locus_tag="RH222_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDV3798260.1"
                     /translation="WKYRFNFYDQHGFPGFWGATPEYKAAFKALKVRQRLTIQMNFIA
                     FFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVAAYVAVNTNKWFYEK
                     EVKGLNTWSL"
BASE COUNT         3778 a         4283 c         4433 g         3990 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacgg
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg catcagaaag gtcccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc
      541 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tctgctggcg tagcactttt tccagctgtt ccgggttatt ggttttcaga
      661 cgcgggatcg gcagcagtag gcgatcgtca tactggcgtt tcaggccgtc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcgcta
      841 ccgttatcgg cacgagcctg atccatcagg ccaatccatg cctgttgttc cagcattgcg
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggataatatc cagcagcgaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctccgct
     1141 gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttcc gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgg
     1321 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gcgcgccagt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcggtct ggatcagcac ataaccctga
     1501 tgaccggcaa tcatcgggcc aaccacgatc cccgcaatca gcaacacaaa gagcaataac
     1561 acttttagca tggttattct ccttgcggcg cagctgccga agtatcagct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc
     1681 ccgctgccgg ttgtgccagc aggttacgca cgcgagtctg catcagtttt tccagcatcg
     1741 cctggctttg cagggtttcc ggaagatcca tcgagatatt ttgctggctt aactggtcca
     1801 cctcgtcgag gaacgctttg gtggtggcat catcagtatc gtagtaagca cgtacccagg
     1861 tggagacgtt ctccagcgcc tggcgataag tctcttgctg gtgacgcggt acagcttgtg
     1921 ctgcgaccag caggcgagag cgaatatttt cgcgcagata gatatcctga tttggcgcta
     1981 acagcggtac ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc ataaaattct
     2041 gccagctttt ttgcagattg atacgccatt cgctgatgga actggaaagc tcttcaccgt
     2101 cggaatccat cggcgaacca tcgctgtcat tatcggccag acgcaggtta tctacctgat
     2161 ttgaaagctg attgagctta aggatgatgc cgtcataatc cacctgcgat accgcagaaa
     2221 ggctggcgat atcatcggta attgcccggc gaacggtaat cagactcggg tcattcatat
     2281 ccgccaggct ggcatctgca cttttcagca acgctgcagc ggtcgtgacg tcttgatcgc
     2341 tccacagctt ccgtccggcg agtttcacca gaaaatcggc ctgagccagc agccaggttt
     2401 tagcatcgct gccggaaatg gtggcgacct tttgttggac ttcatccaac tgttttgcca
     2461 gcgtttcttg ctgacgattc gcctgcttaa gctgtgcagc ttgttgctta ataatgcctt
     2521 ccagctcggc tttttggctc tcctgggctt tttgcaatgc cgtcagttgg ttagccaggg
     2581 catcgctggt ggcggtctga ttgacggcct gttgtttacc ccagccatac aaaccgacgc
     2641 ccgccgccag agcaatagcg atagccaccg cgctgagaat caatgcggta ttgttcttac
     2701 tctttttttc tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg gtctcttcaa
     2761 ccacggcgga ggttttttct tgttccgtca ttatggcttc ctgttatgag agttattgta
     2821 atgcccgtaa aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct tgccagccca
     2881 gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag tgtagtaacc
     2941 agtgctcacg ataccattgt gggatcagcg accagagttg ctgcaacatt tcaccgctgg
     3001 taacaacgac cgtcgtcacc tcgcgggatt gccagcgcat cgcttcttct gcaccatcgt
     3061 aatggattgc gcatcgttga taacattcac aaaaagtgac ctcagcaccg cgcgccgtca
     3121 gggtatcccc aattagctca cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg
     3181 caatattttg taattcaggt aattgtagca agacttcgct gatttcccga tcctgcgggt
     3241 agagaatctt ctgtccgctt acggtatgta gtgccagtgc ggtggtgcgt ccaatggcga
     3301 aataattagg tagtcggggc catttacgat cttgctgatg tagctgtgat tgggcaaaag
     3361 caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc ccccagcgct gccagttgat
     3421 cagcaagttg cggtaattgt cgacccggag aaaactcaat cagcggaaaa tgccaggcca
     3481 cctgtcccag tgtgcgcaga cggctcacta actcttctcc agcgggagac gggcgggtga
     3541 ccaggatact catgccgggg cgtctccgtt atagacttca gcgaggatct cgcgcgcgcc
     3601 gttattcagt agctcttctg ccagcgaaat ccccatttgt tcggcatctt gcggcgcacc
     3661 gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg cacgtagcca
     3721 gatttcgcca tcaataagct cggcgtagct accaattggc acctgacatc cgccttcgag
     3781 acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt gattcagcgc
     3841 ggcaagcagc tcgcgagtgc gtgcatcatc aaggcggcat tcaataccca ccgcaccttg
     3901 ccctaccgcc ggaagagaaa tctcgggcgg taacgcggcg cgaatgcggg actccagacc
     3961 taaacgtttt agtccggcta cggcaagaat gatggcatcg tattcgccgt tatccagttt
     4021 actcaggcga gtgccgacgt tgccgcgcag ggagcggata atcagatccg ggcggcgttc
     4081 agccagttgg cactggcgac gtaaactgga cgtcccgacg atactgcctg ccggtaacgc
     4141 atccagactg tcatagttat tggacacaaa ggcatcgcga ggatcttcac gctcacaaat
     4201 agtgaccagt cccagacctt gcggaaattc aaccggcaca tctttcattg agtgtacggc
     4261 gatatcggcg cgattttcga ggagcgcgac ttccagctct tttacaaata agccttttcc
     4321 acctactttc gccagcggcg tatcaagaat cacatcgccg cgcgtcacca tcggtaccag
     4381 ttcaacgacc aggcccggat ggctcgccat caacttgtct ttgacatagt gtgcctgcca
     4441 gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac
     4501 cgtcattatc atccgtggtt catcctaaca tccttgccag agtgatgtca gtgttgtggt
     4561 gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc tgacaggcgt
     4621 ttcacgccgt cgtaataagg aatttacaga gaataaacgg tgctacactt gtatgtagcg
     4681 catctttctt tacggtcaat cagcaaggtg ttaaattgat cacgttttag accatttttt
     4741 cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt gacatacgaa
     4801 atatcccgaa tgccgcgtgt taccgttgat gttggcggaa tcacagtcat gacgggtagc
     4861 aaatcaggcg atacgtcttg tacctctata ttgagactct gaaacagaga ctggatgcca
     4921 taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc caacaggtct
     4981 acagtctact gccgacattg ttgcactatc accatccgct aatgccgggt taccttgatg
     5041 gtaacgttcc caaaggcatt tgcctttaca cgcctgatga aactcaacgc cactacctga
     5101 acgagcttga actgtatcgt ggaatgtcag tacaggaccc gccgaaaggt gagcttccaa
     5161 ttactggtgt atacaccatg ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc
     5221 tggatatctg ggtctgtcat caatcctggc tcgatagcga agagcgccag ttgctacaac
     5281 gtaaatgtag tctgctggaa agctgggccg cctcgctggg tgtggaagtc agcttcttcc
     5341 tgattgatga aaaccgcttc cgtcataatg aaagcggcag cctggggggc gaagattgtg
     5401 gctccaccca gcatatactg ctgcttgacg aattttatcg taccgccgtg cgtctcgccg
     5461 gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga agaacattac gacgactatg
     5521 tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga atggctggat ctcggtggct
     5581 taagctcgct ttctgctgaa gagtactttg gtgccagcct ttggcagctc tacaagagta
     5641 tcgattcccc atacaaagcg gtgctgaaaa cactgctgct ggaagcctat tcctgggaat
     5701 acccgaaccc acgtctgctg gcgaaagata tcaaacagcg tttgcacgac ggcgagattg
     5761 tatcgtttgg tctcgatcca tactgcatga tgctggagcg tgttactgaa tacctgacgg
     5821 cgattgaaga ttttacccgt ctggatttag tacgtcgctg cttctattta aaagtgtgcg
     5881 aaaagctcag ccgtgaacgc gcctgcgtag gctggcgtcg cgcagtgttg agccagttag
     5941 tgagcgagtg gggttgggac gaagctcgtc tggcaatgct cgataaccgc gctaactgga
     6001 agattgatca ggtgcgtgag gcgcacaacg agttgctcga cgcgatgatg cagagctacc
     6061 gtaatctgat ccgctttgcg cgtcgcaata accttagcgt ctccgccagt ccgcaggata
     6121 tcggcgtgct gacgcgtaag ctgtatgccg cgtttgaagc attaccaggt aaagtgacgc
     6181 tggtaaaccc gcagatttca cccgatctct cggaaccgaa tctgaccttt atttatgtgc
     6241 cgccgggccg tgctaaccgt tcaggttggt atctgtataa ccgcgcgcca aatattgagt
     6301 cgatcatcag ccatcagccg ctggaatata accgttacct gaataaactg gtggcgtggg
     6361 catggtttaa cggcctgctg acctcgcgca cccgtttgta tattaaaggt aacggcattg
     6421 tcgatttgcc taagttgcag gagatggtcg ccgacgtgtc gcaccatttc ccgctgcgct
     6481 tacctgcacc gacaccgaag gcgctctaca gcccgtgtga gatccgccat ctggcgatta
     6541 tcgttaacct ggaatatgac ccgacagcgg cgttccgcaa tcaggtggtg catttcgatt
     6601 tccgtaagct ggatgtcttc agctttggcg agaatcaaaa ttgcctggta ggtagcgttg
     6661 acctgctgta ccgcaactcg tggaacgaag tgcgtacgct gcatttcaac ggcgagcaat
     6721 cgatgatcga agccctgaaa actattctcg gcaaaatgca tcaggacgcc gcaccgccag
     6781 atagcgtgga agtcttctgt tatagccagc atctgcgcgg cttaattcgt actcgcgtgc
     6841 agcaactggt ttctgagtgt attgaattgc gtctttccag cacccgccag gaaaccgggc
     6901 gtttcaaggc gctgcgcgtt tctggtcaaa cctgggggtt gttcttcgaa cgcctgaatg
     6961 tatcggtaca gaaactggaa aacgccatcg agttttatgg cgcgatttcg cataacaaac
     7021 tgcacggcct gtcagtgcag gttgaaacca atcacgtcaa attaccggcg gtggtggacg
     7081 gctttgccag cgaagggatc atccagttct ttttcgaaga aacgcaagac gagaatggct
     7141 ttaatatcta cattctcgac gaaagcaacc gggttgaggt gtatcaccac tgcgaaggca
     7201 gcaaagagga gctggtacgt gacgtcagtc gcttctactc gtcatcgcat gaccgtttta
     7261 cctacggctc aagcttcatc aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg
     7321 atggtcgtga acaggtgatt ccgttccgca caaaatctat cggtaacatg ccgcctgcca
     7381 atcaggatca cgatacgccg ctattacagc agtatttttc gtgatgaacg tgccggaaag
     7441 cgaggcttat ccggcatgca atcttagcgg aaactgactg tttcacccgc ctgctgcgtc
     7501 gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat ccactcatcg
     7561 cctttcagat caaaatggta gccgccttgt ttggttgcca gccatacctg gtgcagcggt
     7621 tcctgtcggt tgataatgat tttgctgccg ttttcaaagg taatggtcag tacgccgccg
     7681 ttgatttcgc agtcgatatc gctgtcgcca tcccagtcgt ccaggcgttc ttcaatggtc
     7741 agccagagtt gatcagccag gcgatgaaat tcactgtcgt tcattgttgt atcctgtttt
     7801 taagtgatgg cggcagtata gcggcatggg gtcagggctt caaagtttgc acctctgcgg
     7861 ctgcgttccg gcacgattca tccgtcaccg gaataatgat gtctcagtgt agcgaaagat
     7921 ttgtctcttc attagggcgc agttacacca cgcctttccc tgtttctggt aaacattatg
     7981 atcaggttta ccgagcgagc atcctcacgc tgacggaact aaaaaagcca acaaacaaac
     8041 cacattgcga tagtgcataa agccatcctg gcgcgaggtg ttgatcacga aactgccagc
     8101 agaacataaa tccccacgag taagcgttat actcgcagca tttcctcact tttcagactt
     8161 cataaagagt cgctaaatgc ttgcttttac gccttctcct gcgatgatag aaagcagaaa
     8221 gcgataaact ttacaggcaa tccataatga aaaacgtgtt taaggcactc actgtattac
     8281 ttactctctt cagcctgacg ggctgcggtc tgaaaggtcc gctctatttc ccgcctgcag
     8341 ataaaaatgc accgccgccg accaaaccgg tagagacgca aacgcaatcc acggtgccgg
     8401 ataaaaacga tcgcgccact ggcgatggtc catcccaggt gaattactaa aagtcagttt
     8461 ctgtacccgc gtgattggag taaatgatgc agttctcgaa aatgcatggc cttggcaacg
     8521 attttatggt cgtcgacgcg gtaacgcaga atgtcttttt ttcaccggag ctgattcgtc
     8581 gcctggcgga tcggcacctg ggggtagggt ttgaccagct gctggtggtt gagccgccgt
     8641 atgatcctga actggatttt cactatcgca ttttcaatgc tgatggcagt gaagtggcgc
     8701 agtgcggcaa cggtgcgcgc tgctttgccc gttttgtgcg cctgaaaggg cttaccaata
     8761 agcgcgatat ccgcgtcagc accgccaacg ggcggatggt tctgaccgtc accgatgatg
     8821 atttggtccg cgtaaatatg ggcgaaccca acttcgaacc ttccgctgtg ccgtttcgcg
     8881 ctaacaaagc ggaaaagacc tatattatgc gcgccgccga gcagacaatc ttatgcggcg
     8941 tggtgtcgat gggaaatccg cattgcgtga ttcaggtcga tgatgtcgat accgcggcgg
     9001 tagaaacgct tggtcctgtt ctggaaagcc acgagcgttt tccggagcga gccaatatcg
     9061 gttttatgca agtggttaag cgcgagcata ttcgtttacg cgtttatgag cgtggggcag
     9121 gagaaaccca ggcctgcggc agcggcgcgt gtgcggcggt tgcagtaggg attcagcaag
     9181 gtttgctggc cgaagaagta cgcgtggaac tccccggcgg tcgtcttgat atcgcctgga
     9241 aaggtccggg tcacccgtta tatatgactg gcccggcggt acatgtctac gacggattta
     9301 ttcatctatg aagcaaccag gggaagaact gcaggaaaca ctcacggagc ttgatgaccg
     9361 ggcggttgtc gattatctga ttaaaaatcc tgagtttttt atccgcaatg cgcgcgcagt
     9421 agaagcgata cgtgtgccgc atccggtacg cggcaccgtt tcgttggtcg agtggcacat
     9481 ggcccgcgca cgtaatcata ttcatgttct ggaagagaac atggtgctgt tgatggaaca
     9541 ggctatcgcc aacgaaggcc tgttttatcg cctactctac ctgcagcgca gtctcaccgc
     9601 cgccagcagt ctcgacgata tgctgatgcg ctttcaccgc tgggcgcgcg atctcggcct
     9661 ggcaggtgcg agtctgcgcc tgtttccgga tcgctggcgc ttaggtgcgc cgtcgaacca
     9721 cactcatctg gcattaagcc gtcagtcttt cgaaccgctg cgtattcagc gtttggggca
     9781 ggaacagcac tatcttgggc cgcttaacgg accagagctg ctggtggtgc taccggaagc
     9841 gaaagcggtg ggatcggtgg cgatgtcgat gctgggaagc gatgctgatt tgggtgtcgt
     9901 gctgtttacc agtcgtgatg ccagtcacta tcaacaaggg cagggaacgc agttacttca
     9961 tgaaattgcg ctgatgttgc cggagcttct ggagcgttgg attgaacgcg tatgaccgat
    10021 ttacacaccg atgtagaacg ctacctacgt tatctgagcg tggagcgtca gcttagcccg
    10081 ataaccctac ttaactacca gcgtcagctt gaggcgatca tcaattttgc cagcgaaaac
    10141 ggcctgcaaa gctggcaaca atgcgatgca gcgatggtac gcaattttgc tgtacgcagt
    10201 cgccgtaaag ggctgggagc agcaagtctg gcgttacggc tttctgcgct acgtagcttt
    10261 tttgactggc tggtcagcca gaacgaactc aaagctaacc cggcgaaagg agtttcggca
    10321 ccgaaagcgc cgcgtcatct gccgaaaaac atcgacgtcg acgatatgaa tcggctgctg
    10381 gatattgata tcaacgatcc cctcgctgta cgcgaccgtg caatgctgga agtgatgtac
    10441 ggcgcgggtc tgcgtctttc cgagctggtg gggctggata tcaaacacct cgacctggag
    10501 tctggtgaag tgtgggtaat ggggaaaggc agcaaagagc gccgcctgcc gattggtcgc
    10561 aacgctgtgg cgtggattga gcactggctt gatttgcgcg acctgtttgg tagcgaagac
    10621 gacgcgcttt ttctgtcgaa actgggcaag cgtatctccg cgcgtaatgt gcagaaacgc
    10681 tttgccgaat ggggcataaa acaagggctg aataatcacg ttcacccgca taaattacgt
    10741 cactcgttcg ctacgcatat gctggagtcg agcggcgatc ttcgtggtgt gcaggagctg
    10801 ctgggtcatg ccaacctctc caccacgcaa atctatactc atcttgattt tcaacacctt
    10861 gcctcggtgt acgatgcggc gcatccacgc gccaaacggg ggaaataatg cgtttttacc
    10921 ggcctttggg gcgcatctcg gcgctcacct ttgacctgga tgataccctt tacgataacc
    10981 gtccggtgat tttgcgcacc gagcgagagg cgcttacctt tgtgcaaaat tatcatccgg
    11041 cgctgcgcag cttccagaat gaagatctgc aacgcctgcg ccaggcggta cgggaagcgg
    11101 aacccgagat ttatcacgac gtgacgcgct ggcgttttcg ttcgattgaa caagcgatgc
    11161 tcgacgccgg gctgagtgcc gaagaagcca gtgcaggcgc acacgcagca atgatcaact
    11221 ttgccaaatg gcgcagccga atcgacgtcc cgcagcaaac tcacgacacc ttaaaacagc
    11281 tggcgaagaa atggccgctg gtggcgatca ccaacggtaa cgcccagccg gagctgtttg
    11341 gtttggggga ttattttgag tttgtgctgc gcgctggccc gcacgggcgc tcaaaaccgt
    11401 tcagcgatat gtactttttg gctgcggaaa aactcaacgt gccgatcggc gagatcttac
    11461 atgttgggga cgatctcacc actgacgtgg gtggggcaat tcgcagcgga atgcaggctt
    11521 gttggatcag accggaaaat ggcgatctga tgcaaacctg ggacagccgt ttactgccgc
    11581 atctggaaat ttcccggttg gcatctctga cctcgctgat ataatcagca aatctgtata
    11641 tatacccagc tttttggcgg agggcgttgc gcttctccgc ccaacctatt tttacgcggc
    11701 ggtgccaatg gacgtttctt acctgctcga cagccttaat gacaaacagc gcgaagcggt
    11761 ggccgcgcca cgcagcaacc ttctggtgct ggcgggcgcg ggcagtggta agacgcgcgt
    11821 actggtgcat cgtatcgcct ggttgatgag cgtggaaaac tgctcgccat actcgattat
    11881 ggcggtgacg tttaccaaca aagcggcggc ggaaatgcgt catcgtatcg ggcaactgat
    11941 gggcaccagc cagggcggca tgtgggtcgg caccttccac gggctggcgc accgtttgct
    12001 gcgtgcgcac catatggacg ccaatctacc gcaggatttc cagatcctcg acagtgaaga
    12061 ccagctacgc ctgcttaagc gtctgatcaa agccatgaac ctcgacgaga agcagtggcc
    12121 gccgcgccag gcaatgtggt acatcaacag ccagaaagat gaaggcctgc gtccgcatca
    12181 tattcaaagc tacggtaatc cggtggagca gacctggcag aaggtgtatc aggcgtatca
    12241 ggaagcgtgc gatcgtgcgg gcctggtgga cttcgccgag ctgctgctgc gcgctcacga
    12301 gttgtggctt aacaagccgc atatcctgca acactaccgc gaacgtttta ccaacatcct
    12361 ggtggacgaa ttccaggata ccaacaacat tcagtacgcg tggatccgcc tgctggcggg
    12421 cgataccggc aaagtgatga tcgttggcga tgacgaccag tcaatctacg gctggcgcgg
    12481 ggcgcaggta gagaatattc agcgtttcct taatgatttc cccggtgccg aaactattcg
    12541 tctggagcaa aactaccgct ctaccagcaa tattctgagc gcggctaacg ccctgattga
    12601 aaataacaat gggcgcttag gtaaaaaatt gtggaccgat ggcgcggacg gtgagccgat
    12661 ttctctttat tgcgccttta acgaactcga cgaagcgcgt tttgtggtta accgcatcaa
    12721 aacctggcag gataacggcg gagcgcttgc cgagtgcgcc attctctacc gcagtaacgc
    12781 ccagtcgcgc gtgctggaag aagcactgtt gcaggcgagt atgccgtacc gtatttacgg
    12841 cgggatgcgc ttcttcgaac gccaggaaat caaagatgcg ctctcgtatc tgcgcctgat
    12901 tgccaaccgc aacgacgacg cggcctttga gcgcgtagtg aatacaccaa cgcggggtat
    12961 tggtgaccgg acgctggacg tggtacgtca gacatcgcgc gatcgccagt taacgctgtg
    13021 gcaggcgtgc cgtgaactgt tgcaggaaaa agcgctcgcc ggacgtgcag ccagcgcctt
    13081 gcagcggttt atggaactga tcgacgcctt agcgcaagaa actgccgata tgccgctaca
    13141 tgtacagact gaccgggtca ttaaagactc cggcctgcgc accatgtacg agcaggagaa
    13201 gggcgaaaaa ggtcagacgc gtatcgaaaa cttggaggaa ctggtgacgg caacgcgcca
    13261 gttcagctac aacgaagaag atgaagattt aatgccgttg caggcgtttc tctcccatgc
    13321 ggcgctggaa gcgggcgaag ggcaggcgga tacctggcag gacgcggtgc agttgatgac
    13381 gctacactcg gcgaaaggtc tggagtttcc gcaggtgttt attgtcggta tggaagaggg
    13441 catgttccca agccagatgt cgctggatga aggcgggcgt ctggaagaag aacgccgtct
    13501 ggcctacgtt ggcgtaaccc gcgcgatgca gaaactgacg ttgacctacg cggaaactcg
    13561 ccgtctgtat ggcaaagagg tttaccatcg cccgtcgcgc tttatcggcg agctgccgga
    13621 agagtgtgtg gaagaggtgc gcctgcgcgc tacggtaagt cgtccggtca gccatcaacg
    13681 gatggggacg ccgatggtcg agaacgacag cggctacaag ctcggtcagc gtgtacgcca
    13741 cgctaagttt ggtgaaggca ccatcgtcaa tatggaaggc agcggtgaac atagccgttt
    13801 gcaggtggcg ttccagggcc agggaatcaa atggctggtg gcggcttacg cccggctgga
    13861 gacggtgtaa cgttgccgga tgcggtgctg cgcaccttat ttggcctaaa aaatcattca
    13921 gattcaataa attgcaacgt catgtaggcc ggataaggcg tttacgccgc atccggcatc
    13981 agcagcatat ttagtatctg acacaaaact atcattctaa cctttccgct cgacggaaat
    14041 catggtgaca aaaggatagc gttgccacgg gattgccccg cctttcatat acatatgtga
    14101 aatcgtgcca tcaagataaa gcagctgctc aacgttcagt ttcgctttgg cataacagga
    14161 aaaatcataa aaatttgttg cctgctggct caacaaaaac acggcattcc catgtttatt
    14221 aatcccaaca ccgttacgaa ttttacgtga ggcgacgttg ggatgaatac gcggattaat
    14281 tacaccgttt tccatcaaca ttggccctga ctgcaccgca aactgaatct ctttactggc
    14341 tttaaaggca tccagacgaa cgatgccgac tttatctccc gcgacataaa acacgccgcc
    14401 aggacggata aagaaattcc cttcacctga agcgagattt aacgccacct tctgctgacc
    14461 gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc cgttcatcgc
    14521 catctgcacc tgaccctgac tattcatatc caccagcaga gcatgtaaag ttccccacgc
    14581 ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat taacggtata
    14641 cgcctgtacg atcagcatcg gatctgagag tgcgcaatca tcagcggcaa cagcaaacaa
    14701 gggaagtaag gtgagggcga gaaaaatccg tttgagattc aaggtgatca ttcctttacc
    14761 aatgagtagc cgatgcgcca ttataggtcc tggatgtggg atttttttat cctgttagcg
    14821 accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt tctgttgcgt
    14881 gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag aggacattcg
    14941 ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc aactcctaag
    15001 gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt tcccgtcgcg
    15061 ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc accgaactgt
    15121 ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact ggaaaataac
    15181 cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt atggattgat
    15241 cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg ccagagcctg
    15301 gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga agacgacgac
    15361 ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc cggtaactcc
    15421 actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg tgaactgccc
    15481 gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg taacgcctac
    15541 gagctgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga aattgaaaat
    15601 atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca gggcgatgag
    15661 tacgacgagg cgctctccac tctggcggaa ctggaagata tcggctggaa agtgcgcctg
    15721 tgtctgatgg atacccagcg tgcgctcaac ttcctggtgc gtaaagcgcg tttaccgggt
    15781 gggcaactgg agcaggcgcg tgaaatcctg cgagatatcg aatccctgct gccgcataac
    15841 gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt tatcaacatc
    15901 gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct gccgccaacg
    15961 ctggttgctt ccagctatgg gatgaacttt gagtttatgc cagaactgaa gtggagtttc
    16021 ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta tctgtacttt
    16081 aagcggaaga actggttgta aaaaacgaga gcggtggctt agtctggcta agccacctgt
    16141 tattcaaagg ctccaggtat ttaacccttt tacctctttc tcatagaacc atttgttcgt
    16201 gttaacagca acataggctg ctacggcaat tcccagaatg ttaacgccaa ttaatgcacc
    16261 aacaaataga gatagaatgc caagtaatag aactataatt gcttttttcc ataagcccag
    16321 aacaaataaa tagatccagg aacagaagaa agcaatgaaa ttcatttgaa tagttaatcg
    16381 ttgtcttact tttaatgctt taaatgctgc tttatattct ggtgttgctc cccagaatcc
    16441 aggaaagcca tgttgatcat aaaaattaaa tcggtatttc catt
//
LOCUS       DAPHJE010000097        16404 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPHJE010000097 DAPHJE010000000
VERSION     DAPHJE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.098"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            398..1087
                     /gene="mutH"
                     /locus_tag="RH222_003284"
     CDS             398..1087
                     /gene="mutH"
                     /locus_tag="RH222_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV3798261.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            1156..1869
                     /locus_tag="RH222_003285"
     CDS             1156..1869
                     /locus_tag="RH222_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV3798262.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            2007..2225
                     /gene="ygdR"
                     /locus_tag="RH222_003286"
     CDS             2007..2225
                     /gene="ygdR"
                     /locus_tag="RH222_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV3798263.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            2333..3373
                     /locus_tag="RH222_003287"
     CDS             2333..3373
                     /locus_tag="RH222_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV3798264.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="RH222_003288"
     CDS             complement(3405..4598)
                     /gene="lplT"
                     /locus_tag="RH222_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV3798265.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(4591..6750)
                     /gene="aas"
                     /locus_tag="RH222_003289"
     CDS             complement(4591..6750)
                     /gene="aas"
                     /locus_tag="RH222_003289"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV3798266.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            7336..8367
                     /gene="galR"
                     /locus_tag="RH222_003290"
     CDS             7336..8367
                     /gene="galR"
                     /locus_tag="RH222_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV3798267.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTSNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(8374..9636)
                     /gene="lysA"
                     /locus_tag="RH222_003291"
     CDS             complement(8374..9636)
                     /gene="lysA"
                     /locus_tag="RH222_003291"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV3798268.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            9758..10693
                     /gene="lysR"
                     /locus_tag="RH222_003292"
     CDS             9758..10693
                     /gene="lysR"
                     /locus_tag="RH222_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV3798269.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(10680..11372)
                     /gene="ygeA"
                     /locus_tag="RH222_003293"
     CDS             complement(10680..11372)
                     /gene="ygeA"
                     /locus_tag="RH222_003293"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV3798270.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(11501..12919)
                     /gene="araE"
                     /locus_tag="RH222_003294"
     CDS             complement(11501..12919)
                     /gene="araE"
                     /locus_tag="RH222_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV3798271.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(13234..13995)
                     /gene="kduD"
                     /locus_tag="RH222_003295"
     CDS             complement(13234..13995)
                     /gene="kduD"
                     /locus_tag="RH222_003295"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV3798272.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(14025..14861)
                     /gene="kduI"
                     /locus_tag="RH222_003296"
     CDS             complement(14025..14861)
                     /gene="kduI"
                     /locus_tag="RH222_003296"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV3798273.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(15148..16329)
                     /locus_tag="RH222_003297"
     CDS             complement(15148..16329)
                     /locus_tag="RH222_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV3798274.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
BASE COUNT         4223 a         4393 c         3944 g         3844 t
ORIGIN      
        1 ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
       61 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
      121 ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
      181 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
      241 ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc ctaaaaagca
      301 atataaatca gagaattgaa caacacatgt ggaaaaagtt acactgcgaa tattcggcac
      361 ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct
      421 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
      481 attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa aacgcgataa
      541 gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga gtaaacctga
      601 gcaagatttt gctgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg
      661 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg
      721 ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga
      781 gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga
      841 agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
      901 tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc
      961 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
     1021 acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat
     1081 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
     1141 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
     1201 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
     1261 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
     1321 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
     1381 ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt
     1441 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
     1501 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
     1561 gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt
     1621 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
     1681 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg
     1741 gtcggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
     1801 ttcgcgatgt tcttctctat tgcggttgaa agcctcaacc tgattcgcaa caaaaagaac
     1861 ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctg ttaccctcac
     1921 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
     1981 taggtgggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
     2041 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
     2101 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
     2161 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
     2221 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
     2281 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
     2341 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
     2401 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
     2461 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
     2521 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
     2581 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
     2641 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
     2701 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa
     2761 actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc
     2821 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
     2881 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac
     2941 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
     3001 ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg
     3061 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
     3121 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
     3181 tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc
     3241 cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg aaagtttgca
     3301 tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg tttatactta
     3361 tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
     3421 cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat gccaatgggc
     3481 acgaccggga tgcctatcat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
     3541 ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
     3601 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac gccaatcagc
     3661 atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag taccaccacg
     3721 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac taacttcgct
     3781 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtgggcgta
     3841 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
     3901 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcgc
     3961 catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc
     4021 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
     4081 gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac accaccggct
     4141 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agctttcact
     4201 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
     4261 gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
     4321 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
     4381 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
     4441 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
     4501 agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
     4561 gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
     4621 ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag
     4681 atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg
     4741 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct
     4801 ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
     4861 ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc
     4921 ctgaatctgc acaaagccca gctcgtcaaa acgcacaata tcgccagtgt cataccagcc
     4981 gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc
     5041 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
     5101 gataccaggg accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
     5161 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg tcacaccgta
     5221 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtag
     5281 tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt tggcgaaacg
     5341 cgcgtagtga ccgaggaaag tcgaagtgcc gaacaacacg gtgcaactgc ggtcatacac
     5401 caactccggc acaatacggt aatgcagcgg gcttggataa agaaacactt ctgcacctgt
     5461 gagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga
     5521 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cattcgccag
     5581 aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg aggtaaaaag
     5641 gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc agacgcggca tcagcaaatg
     5701 agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
     5761 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
     5821 gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca gcccttttac
     5881 cccggcagtg tagttcatca ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac
     5941 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
     6001 tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat aggagtctgg
     6061 ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcactcag
     6121 taaagattcg tacagcgttt cacgcgggcg caccgccatt cgcgcttcca tcattatttg
     6181 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc
     6241 cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
     6301 acctttcagg cggctgaagt gcgtgagttc cgccccttca atacgcacag gaataaccgt
     6361 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagcctgt
     6421 agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac
     6481 cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
     6541 cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca ctggacgcac
     6601 aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag gcgtaattag
     6661 aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg
     6721 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
     6781 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
     6841 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
     6901 aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca
     6961 gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg
     7021 ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat
     7081 gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac tttagcaacc
     7141 tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt
     7201 ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac
     7261 acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta agcgtttacc
     7321 cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc
     7381 gccaccgttt cccgcgtcat taataattca cctaaagcca gtgaagcttc ccggctggct
     7441 gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggcg
     7501 cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc gtttttcggt
     7561 gcaatggtga aagcggtcga acaggtggct tatcacacca gtaatttctt attgattggc
     7621 aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc
     7681 tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc ctcattaatg
     7741 aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt
     7801 attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt
     7861 catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga agatcgtctg
     7921 caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca
     7981 tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt gggacgagga
     8041 agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc gatgggcgtt
     8101 ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt tgatgatgtg
     8161 ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat cgtgacgatg
     8221 gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct cccggaaatc
     8281 actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc gtcgctggag
     8341 gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg ccagtaattc
     8401 ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca gaacctctgg
     8461 taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc ctgtatcatg
     8521 cagtaccaga taatcacctg ccttcacttc cggcaaggcg cgggtttcaa catttccccc
     8581 ttcctgctgg gtaaagacat cgcccgattc acataacggt ccggcgacga cggtttccac
     8641 cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat ggtggtaact
     8701 accgtacatt gccgggcgca tcagatcgtt gaacccagca tcaaccagca caaagtggcg
     8761 gctacccatt tgtttgacgc tccgcacctg agtaattaat acgccagact gcgctaccag
     8821 gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcggg cgatttgctc
     8881 acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg
     8941 ataaggaaca gaaagcccac cgcccgcaga aatagcctgt aaatcctgac cgaattcgat
     9001 gacctgacgc accatagcac cacacacctg ttccagatgg gcataatcaa cgccagaacc
     9061 aatgtgcatg tgaatgccga ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc
     9121 gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat tggttttttg
     9181 gctatgtccg tgaccaaacc ccggattaac gcgcagccat acccgatgcc ctggcgaaac
     9241 ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact
     9301 gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat catcggggtg
     9361 cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc
     9421 cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg
     9481 tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc
     9541 atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat tttcggcggt
     9601 gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat aagtgctttt
     9661 ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg agtttatgca
     9721 aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca
     9781 tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcgg cacacctgct
     9841 acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg tgatcgggct
     9901 gaaattgttt gagcgcgtac gtgggcgatt acatcctacc gtgcaaggac tgcgtctgtt
     9961 tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct
    10021 gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt cgcaatcttt
    10081 tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt
    10141 gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg atttaggact
    10201 cactgaaacg ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga
    10261 agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa cgccggatga
    10321 ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc agttgctgga
    10381 tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc
    10441 gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc
    10501 actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac
    10561 cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga
    10621 gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt tgtcgtcagc
    10681 tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcggtatc aaacacaggc
    10741 aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa aatgacgccc
    10801 tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
    10861 gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc
    10921 gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
    10981 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt
    11041 gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca
    11101 tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc cgcccgctgt
    11161 aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc gcgacgctgg
    11221 cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca
    11281 ccaagccgct gtttaatgcc ttcatttatc aaacgatagt aaggaatggt ggattcccag
    11341 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa
    11401 caaggctgaa taaggaacga gacaaacgcc tcaacggcca agtgcccaat ctctattaac
    11461 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg atatttctca
    11521 acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt ttggtttccg
    11581 gaatgagcca gaaagtaatg cccacaaacg caatgttcag cgcagtgtag agccagaacg
    11641 taccggcagc gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata atcatattcg
    11701 acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa
    11761 tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
    11821 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
    11881 gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
    11941 gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc gcgaacataa
    12001 aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
    12061 ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac tgctgcatcg
    12121 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
    12181 cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
    12241 tttccgacgt atcgcgcagc atacgcaata cttcttccgc ctcaatatga cgcccctttt
    12301 ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc agaactgctg
    12361 gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg
    12421 ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg atcatcttac
    12481 cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc
    12541 cgacagcaat gcccagcacc acccgagcgg cgattaacat ctctacgctg gtcgcaaaag
    12601 cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgcc
    12661 ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc
    12721 tactaaccac ccattcctgc aaacgactgg tcagcacaaa gtgatcggta atgaacggca
    12781 acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta
    12841 ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt
    12901 ccgtattgat agtaaccatt ttttcctgcc agcagagaat aagccatagt gaaaaaatac
    12961 gtgaacaatt cacacaggtg tcaggtcgga aatagcataa atatggatta aattgctacg
    13021 acatgccgtt atgtgatgga tattccaatt ttaaaattaa gttgaattat tgagattatt
    13081 attaaccacc taattttaaa gcagataaaa ttcataaagt tcattcattg ataattgata
    13141 tggattattt cataaccatg atatggatta tgatgatcta cagatataaa aaaccctgcc
    13201 atgcggcagg gtcataaaag taagaagaat gaattaacgc gccagccaac cgccatccac
    13261 ggcaatggta taaccattca cataatctga agcgctggag gcaaggaaca ctaccggccc
    13321 catcaggtca ctcggcagtc cccaacggcc agctggaatg cggtcgagga tttccgcgct
    13381 acgttgttca tctgcccgta gttgttgagt attgttggtc gccatgtaac ccggggctat
    13441 cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg tcacacccat
    13501 cacgccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga aggagagcat
    13561 tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc
    13621 ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc agtctttttc
    13681 gctgaactcg agagcgtctt cgcggcgaat caatccggcg ttattcacca ggatatcaat
    13741 atgaccaaat tcagctaccg cgcgatccag cagtccagga atgccatcaa tctttcgcag
    13801 atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg acctgctcga tggtttcagt
    13861 cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc ccaacgccat
    13921 cccctggccc agcccagtat cacaaccagt gacgaccgca actttacctt cgagagaaaa
    13981 tgcacttaaa atcataacaa taccttattc gtttatgccc acaactagcg caaatcttta
    14041 acggccacat ggtccatatc atcaaagacc tggttttcac caaccatgcc ccagataaag
    14101 gtataagctt tggttccgac gccggaatgg atcgaccagc tcggggagat caccgcctgc
    14161 tcgttatgca tcacaatgtg acgcgtttct tgcggttgcc ccatcatgtg gaaaacgcag
    14221 gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg ggtgtgacac
    14281 ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact caattggcag
    14341 gtttccagca catccgggac aaaatatttg ttaatagtgc gacggttact ggtgaggtta
    14401 tcgcctaacg tgactggaga tacttcgtcc ggggtgactt ttttggtggg atacgtcgta
    14461 tgcgcgggtg cgcaattgta ataaaacttc gccggagtgc cggtatcaat actggcaaag
    14521 acaacttctt ttgcaccttt accaacatac agggcgtcgc ggtgaccgat ttcatagcat
    14581 tggccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa ctcgcgacgt
    14641 tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga aaccgtttta
    14701 gttatcggca taatgccgcc aacaataatt cggtcaatgt ggctgtaaac catggtgtac
    14761 tcatcggcga caaatacctt ttcaaccaaa aattcattgc gcagcccttg ggtatccagc
    14821 gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca catcaacctc cgataatcag
    14881 tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta taaagggatg
    14941 atatgcccat gggctagggt tttcaattaa aaataaaaca atgtttcatt tttaagttaa
    15001 ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg agaatggaaa aatgagggag
    15061 cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat gacggcagat
    15121 atttatcggt aaggaggaaa tgcagagcta ttcgtcacgt tcaatggtca atgccacacc
    15181 ctggcccccg ccgatacaaa gcgttgccag tcctttgcgg gcattacgtt tcaccatttc
    15241 atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac caagtgcgat
    15301 cgcaccacca ttgacattga cccgacgctc atcccactca agcatcttgc caaccgaaag
    15361 cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca actgccagcc
    15421 tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca atgccggatc
    15481 tacaccaacg ctggcaaatg cgcgaatgcg ggccagcacg ggtaaattca acgctcgtgc
    15541 tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg atgcattacc
    15601 cgctgtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac cttctgcact
    15661 ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt tactttgggt
    15721 cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc gcgctttttg
    15781 ttgcgagcta agtgcgtaag catcctgcaa ttgacggctg atgccatatt cgcgagccag
    15841 attttcggcg gtgacaccaa tatgataatc attgaaggca tcccataacc catcatgcac
    15901 aagactgtca accagctggc tattgccaag ctgtgcaccg gtgcggctat cagtcagaac
    15961 atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag cctcgccaca
    16021 ctgtatcgcc tgagtagcca gatgcagtgc tttaagtccg gaaccgcaga cgtcattaat
    16081 cgtgattgca gaaacgctat taggcagacc acctttaata gccgattgcc ttgccggatt
    16141 ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg catatgcagg
    16201 aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca cggcggaatg
    16261 acccgctaac gcaccgcgaa agcagccgat aggtgtccgt aacgccccga caatcacaac
    16321 gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg ttaattacag
    16381 ttatcttaat tgtttaaaaa aagt
//
LOCUS       DAPHJE010000098        16140 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPHJE010000098 DAPHJE010000000
VERSION     DAPHJE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.099"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            337..1956
                     /locus_tag="RH222_003298"
                     /pseudo
     CDS             337..1956
                     /locus_tag="RH222_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            2036..2328
                     /locus_tag="RH222_003299"
                     /pseudo
     CDS             2036..2328
                     /locus_tag="RH222_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2368..3402)
                     /locus_tag="RH222_003300"
     CDS             complement(2368..3402)
                     /locus_tag="RH222_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDV3798275.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIIDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(3405..4370)
                     /locus_tag="RH222_003301"
                     /pseudo
     CDS             complement(3405..4370)
                     /locus_tag="RH222_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(4427..5185)
                     /locus_tag="RH222_003302"
     CDS             complement(4427..5185)
                     /locus_tag="RH222_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDV3798276.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPEHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNAPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(5199..6413)
                     /locus_tag="RH222_003303"
     CDS             complement(5199..6413)
                     /locus_tag="RH222_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV3798277.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGSLASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(6609..6947)
                     /locus_tag="RH222_003304"
                     /pseudo
     CDS             complement(6609..6947)
                     /locus_tag="RH222_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8464..9009
                     /gene="cobU"
                     /locus_tag="RH222_003305"
     CDS             8464..9009
                     /gene="cobU"
                     /locus_tag="RH222_003305"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV3798278.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            9006..9749
                     /gene="cobS"
                     /locus_tag="RH222_003306"
     CDS             9006..9749
                     /gene="cobS"
                     /locus_tag="RH222_003306"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV3798279.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYVREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            9761..10840
                     /gene="cobT"
                     /locus_tag="RH222_003307"
     CDS             9761..10840
                     /gene="cobT"
                     /locus_tag="RH222_003307"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV3798280.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            10902..11837
                     /gene="ldtA"
                     /locus_tag="RH222_003308"
     CDS             10902..11837
                     /gene="ldtA"
                     /locus_tag="RH222_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3798281.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(11893..11968)
                     /locus_tag="RH222_003309"
     tRNA            complement(11893..11968)
                     /locus_tag="RH222_003309"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11933..11935),aa:Asn,seq:gtt)
     gene            12295..13212
                     /gene="nac"
                     /locus_tag="RH222_003310"
     CDS             12295..13212
                     /gene="nac"
                     /locus_tag="RH222_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV3798282.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEFN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            13314..14264
                     /gene="cbl"
                     /locus_tag="RH222_003311"
     CDS             13314..14264
                     /gene="cbl"
                     /locus_tag="RH222_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV3798283.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(14302..14377)
                     /locus_tag="RH222_003312"
     tRNA            complement(14302..14377)
                     /locus_tag="RH222_003312"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14342..14344),aa:Asn,seq:gtt)
     gene            14382..16025
                     /gene="yeeO"
                     /locus_tag="RH222_003313"
     CDS             14382..16025
                     /gene="yeeO"
                     /locus_tag="RH222_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV3798284.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
BASE COUNT         4105 a         3539 c         4096 g         4400 t
ORIGIN      
        1 tatacagcag ggaggggggg ctgcaggaag gcaactggaa ggataggaac tgagcggttc
       61 agtcgcggaa atcagttgca gagctgtaat gatatctccg ggagtgctgc gttggtaggg
      121 gcatcccggg atagagaaat tttagcactt tgccctgaat gattgtagac gttccgagta
      181 ctcgtaccgg tctgagttat tacagttaga tattcacttt ttctcccgca tacttcacat
      241 ctgtcaacga cggatgaaaa gtggttcttc cgcaacctgg gtggaagaaa atggatgaaa
      301 ctttctggcg aacaaggatc taagctgttt taagttatgg gcaacgcaat gcactctctt
      361 aagacacttc tacagttacc ttgcggatgg cgatgcagtc gacaaattat tagctctgac
      421 ggtataaccc tccatctcca cggaaaacgc aaaacagcac aatgtcctga atgctttaag
      481 cgtagcgact ctgttcatag ttgtcgtcgg cgccggatac agcatctacc ctgctcaggg
      541 cagacgctat ggcttgtatt ttccgtccgc cactggtact gccgtaaccc ggcttgttca
      601 cgtaaaattt ttgccgagtc gcttgctccc ttcgccggtt cacaccagca gtcttcacag
      661 gcgttacaaa atttacaacg tcaactggga ttaatagctg gaggtgaggc tggaaaacgg
      721 gctgcaacgg cagcgggtct ccgttgcagt gctgatactc ttcttcgcag ggttatcaat
      781 accccggaga cgaaacagtc aggcgcgcct catgtcggta ttgatgagtg ggcgtggcat
      841 cggggccact gctgcggtat gttaatagtc aatcttgata ctcaccgtcc cctcgtcctg
      901 cttcccggcc gtgatcagcg tacgctggcg acctggttca gaaaatatcc ggaaatacag
      961 gttgtctcgc gtgatcgcag tggagtctat gcgacagcag cacgtgaagg tgcacctcag
     1021 gccagacagg tggccgatcg atggcacctg ctaaaaaata ttggtgatga gcctgaacga
     1081 atgatgtaca gacatatgcc tctgatacgt cttgttgtca gagagttatc actgaagaaa
     1141 tcacctgagc cagaaatatc tgtgcctgta gcatcgctcc gtcgtctgga acgccttaaa
     1201 cagcacatcc gcaaaaaacg gcatcagcgt tggacagagg ttatggccct gcataacaag
     1261 ggatgtagtt tcagggaaat atcccgtatt acaggcctgt cgcgagtgac agtcagtcgc
     1321 tgggtgggtt caggaacatt ccctgaaatg tcaactaggc ctccaaagcg agggcttctg
     1381 gacccatgga gggagtggtt aaaagagcaa cgagaatgtg gtaattataa ctccggccgg
     1441 atatggcggg aaatggtggc caggggggtt acaggcagtg aaaccatcgt cagggatgct
     1501 gttgccaaat ggcgtaaagg ctggatccca ccggttacta ctgccgcaag acttccttca
     1561 gtgtcccggg taagccgctg gttgatgccc tggagaataa tcaggggtga agaaaattat
     1621 gctttccgat ttattagtct gatgtgtgaa aaagaaccgg agttgaaaat agcgcagcaa
     1681 ctggtactcg agttctaccg tattctgaaa acctaaaata aatcacagct aagcagctgg
     1741 ttcacccggg tccacgaaag cggctcagca gaacttcggt gcgtagctgc ggggatggaa
     1801 gctgatgctg cggctatatg tgaggcaatc agcagtcgct ggagtactgg tgttgtcgaa
     1861 ggtcatgtaa atcgcctgaa ggtgctgata cgccagatgt atggtcgagc cggacttgaa
     1921 ctacttaggc ggagggtaat gagtccactg gcatgaaaat cttcacccag gttgcggaag
     1981 agccactttt gcactgttcg tgacagtatg gcctttatgt actcactttg ttaccggtag
     2041 ctggatcatc actaccgtgc tgcaaatggt ggtgaacagc atcaattgtg gaatgaatgt
     2101 tgccgaagcg accaatgcgc ctcgtttcca ctatcagtgg ttgccggacg agctgcgtgt
     2161 tgaaaagggt ttagtccgga tacgctcaag ctgctggatg caaaaggtca gaaagtggcg
     2221 ctgaatgagg cgatgggcag cacgcaaagc attatggttg ggtcggatgg tgagttgtac
     2281 ggcgcatccg actcgcgctt ggtggatgat ttaacggcgg ggtattaatt gacatccccc
     2341 ggcgcaatgt gccgggggat aatggtttta tgcggtcaat aatcgtccgg gattatcgat
     2401 acagagtttt ttgaggatcg ttttatcgac gccgcgctcc gccagatagg ccagaaaaac
     2461 atctggaaca aatccccagc cattaccgcc atttttagcc cacatttgtt taaggaagac
     2521 atcgtggctg agaacaagct gatcggcgta tcccaactca atgagatgag cgacggcatc
     2581 ggcagtctcc tgcacccctg gcgctattcc ctctttcggg aaggtaatgt ctaggccaat
     2641 catgtcgaat tccagccaga cacctttatc aagcattttc cgctgatacg ccacgtcttt
     2701 tcctgacgga tccgagtgtg cgagagagac tttatttggc gagacgccca tttcacctaa
     2761 cacaatgtcg agtacttcat caccgcgacg aagccagccc ggcatgtgaa tattcatcgc
     2821 cacatgagga ttgttaatct gtgccagcga ggcagcccgc aagctgttat gctcggcttc
     2881 ggtaaatgtc ggtgaaacac ctatttcacc gatcattccg gcacgaatat ccgtatcgcc
     2941 aatcccttga ttcaattctt tatcgatcgt tgtcgccagt tcatcaaccg ttttatgaat
     3001 tctctggctt tcaaattttt ccaggtaggg gccggaggaa gcaacaatat tcagacctgt
     3061 ttttaatgcg acttctcgta atgcctgcgc atctcgtccg atagactcag accctgtggc
     3121 atcaataatg gtgcgaccac cgagcgagat aaagttatta atttcgaaga tcacatcttc
     3181 aataggtttg cggtccatat tatcagcgca gcaataagga tcgtgtttta atgcccactg
     3241 gatttctgcc gtgacttttt tatcaacaag ccgttgtgaa aaaggatagc aaggagcatc
     3301 cacaacagag gaaagatcgt tgaacagatg ttcatgagga agtgttagcc ccatatcttc
     3361 acgggcgacg gggcccgtca cagtttgtag ataatctttc atgattatac ctccattttc
     3421 ttcattgttt tcgaggttct gtattccgga atagtggtaa caattgcacc gaccagtgca
     3481 aacagtgttc cgatgatcgt gaccagataa acgctattgc ccagcgatgg aattaatacg
     3541 tcaatcaata ctgatcccag taactggccc gccgttgaag caacccccag catcagtaac
     3601 cccagacctc tgaccaaaat ggccattaaa ccgatagaca ttagtccaag cgggccgcca
     3661 agatacatcc accagacatc tggtaattga acagtgacgt gccctaatgc gacacgaata
     3721 gctagcgctg tgcctaaaac acaaaatcca acgatgaaat tctaggtgat tgaaaccagc
     3781 atggatcccg tagcttcagc tacttttgca tttccagcgg gctgccatcc tgcaagcaaa
     3841 ccagctaaga acggaagaat ggcaagtaag atgaaagagg tagaatgcca ctgaggtgac
     3901 acaacaaaaa tcgtagcaat aaccgcaaat aatgcaccga tgatgcgata aggggtaaag
     3961 tattttttct cgtcgacacc gataccaaag cgatcgcaaa gtaaaccaga cagcaatagc
     4021 gcggagatca gtgcggtttg aaacgttgcg accccgagtg cgctggctga tgcgccttca
     4081 gaaaatacca ccatcgctcc acagagaccg gcaaaccagt tccagagagg aacttttcgg
     4141 tttttaatca gagaaggaat tgccgcaaat tgttgccggg tttcttttcg cgccataata
     4201 ataaaaaaca tcacgactaa accactggca aaggagataa ctgcgcatgc attaccatcc
     4261 tgtaaccagt gacctaattg accattcacc gctgattgca taggggagag catacccgca
     4321 agaatcgtgg caagcatcca taagggagtt gagtacttat tctttttcat acatcaatcc
     4381 ttctattata gtaaatggag taactggagt taccccatta ttattattat cttgcaatat
     4441 gagtcagtat atttacccac aacgcggtat agaacggcca ggacataaac tgctgtgtac
     4501 cccagtgcgg tgaacagtca ctcataaagc aggctatttt cccgttgtga tagttaccaa
     4561 agaccagtaa tggagcgttg tttattgtta ataccacctc agcattttct ttagcgatcg
     4621 cccggttata acctaggaaa aaaggatatt cagagaaccc tttaataact ggatgttcag
     4681 gttgtgatgg ctgtgcaata acaccctctg gtttttcgac acgatcgtca ccatctaaca
     4741 tcgtaaccgg gagcacatca gccagaactg tatttttata attggctttt gcttcaattc
     4801 ccatgaatga gagatagccg ccaatcatca ataacccacc accgttattc acatattctt
     4861 taatcagttc gagagcgttt ggttttatgc gaagctgata aaaagtatca ttttgtagca
     4921 ggaaagtatt actaccaata tcactaataa cgatggcatc atattgttct aactgtgcca
     4981 catcttcagg gaaggcaatt tgtaccgtat gtgctggcat ataggtgaca tctacctgac
     5041 tatttttcag acactggagt aaccaggtcg caccttcttc atatttactt gaggtaaaac
     5101 tgtcatatcc ttttgaatga atcatatgaa tatgccagga ttccccaata aagagcactt
     5161 ttagtgattt tttggtgtta ttcattctct gtcccttctt aatgcgtaaa aatagtttgc
     5221 tgataatgta tactctgaat tcgatgtatc acagattcat gttcaggcat gtcggaagca
     5281 ttgcttgtct ccaccgctag ggatgcaaag gcgctggcat aacacagtgc tgattccagt
     5341 gattttcctt tcgcaagtcc tgatgccaga gagccgttaa acgcatcccc ggctcctgcc
     5401 gtatttttaa cgaccgctgg aaacgctggc gaatgaatga attttttgcc atcaaatgcg
     5461 agcgagccct tgctaccgag cgtgataacc gtgtttttta cccctttatt gtggatagct
     5521 tctgcagctc gtttagctga ttcaagatcg tgtacgtcta tgccggacag taaaccagct
     5581 tctgtttcat tgggggtgat ataatcaaca tcctgaataa gttcgttgac gatatcatta
     5641 tagggtgccg ggttaataat gaccgggata ctgttttttt gcgccaatgt gatcgcttgt
     5701 tgcagagcgg tataatttgt ttcgagctgc agaagtatga catcagaatt aataatcgct
     5761 tctttctgaa tagttatttc atcggacgac attgtcatgt tggcaccggg atagatcgca
     5821 ataacattat cgccagtacc ttcattaaca aggatcgtgg cagttccggt ttgcgtttct
     5881 ttcgtttgat aaattatact tttataaatc ttagacgaat tcataaagtt gatcgcataa
     5941 tcactaaagt gatctgtacc tacttttgtt atgaaataaa catcagtatc ggcaaagctt
     6001 gctgccagcg cctggttaca gccttttccc cccggtgaaa atataaaatt acttgccaga
     6061 agcgattctc cgatagtggg aagcctcggc aaataactga tgatatcaac attaaacgaa
     6121 ccaataacgc atacttgact tttcatagtt gagtggcctt tttttattcc ctgggctgta
     6181 tcataatttt caagcgcata agcgatttca tttttcgcaa tagtatcaag cgagtctagg
     6241 acgataaaag caccgccatg acatcgagtc agatagccaa gcctggcaag ttcattaaga
     6301 tctgaacgaa tagtttcttt cgttacattg aggctattgg acagatcgga tacttttaca
     6361 gaaccgaatt tactgagtcg tttcaatatt tcatgatggc gttcaaattt catgcttcac
     6421 ctcacttcag ttacgttaat atctatcgat gtatattgat gtgataatca ccataaagtg
     6481 gcggggaaaa gcaaaaaaaa gcaaaaatag cctgagaaaa ggtaagcgtt aatggagaat
     6541 catattgatg cttatttatt ggtgagaact acgttccatg gcaggagttc atcaatacgg
     6601 ttggaaggtt attccggcag tacgctcagg atatggcgca gatacgcttc cggatcaatg
     6661 tcgttcagaa ggcaagtgcc gataagccca tacgacagtg cactgcgctc gccgccgaag
     6721 aacataaagt ttttatttcc gagacagatg gcacaaaggg ctttttccgc ggcattatgt
     6781 ctgcctccgc ctgaccgtca acactgtaat aacagagtgc gccccactga ttcagtacac
     6841 agctgaacca ttctcccagt ctggattttt ggatagcgtg gcatttttct ccaccatgca
     6901 tgcagtgagt ccagtaacag tttgctcttc ttctgcctga ccacaagatg tgctggactg
     6961 gtaaacgatg gggatacctg cttacgcgag agcaataagt ggctggccac aattctcggt
     7021 acaaaactct gctgaatagc agcgattttc ccagcgtggt gcgtcgtttg cattttactg
     7081 gttctaagtt aacaattatc tgttcactaa tgactactct aaaaacttgt atcaggacta
     7141 tcacttattt aagtgatatt ggttgtctgg agattcaggg ggccagtcta taaacttttt
     7201 acaaaaggat gaatgcagaa ggttactcta atggccagag tgatgacaat gatggtcaga
     7261 cgttgacctg cagaaactgc attttaattg gtctataaag tctgcaagag gaatcagggt
     7321 cagatcagtg agtcttatcg cggcccgaag ctatgactga aagaaggctg gatacagaat
     7381 tgcaacaatt tggcaatgtc agaacacaga agctttgcaa gcaggtgaaa ggttatgtct
     7441 agcaaaagga gtatatcaga atacatctac tgatgtacaa aacacaacaa tagcttcatg
     7501 atatataaat acgtgatgac gagtggtact ccgcatggtt atttaacttc tcagcaccac
     7561 taatgatcct gagcaacttc gtatttgggc cattgccatg gagtagaaag ctgtgtctga
     7621 gaatgcagaa tttgagaaaa gaattcgctt tcttggagag cttctgaaaa tggcacgcta
     7681 acaacgtttt gttaaaaaat gtgaatcact tgccggggct gcattttcta ttcgaggaga
     7741 atgttgacgt agatattaca gaaatctcag cgcatcagga taaactgcta ccttaggtca
     7801 accaccaaaa gaaaatacaa tacgtccagt ttgtatgcct ttaataacac atcttctaaa
     7861 tgttgaagaa ttatcccgcc tgctgaagaa cgttgctctg agtgtgataa acctttttat
     7921 tcttgatgat gtcagcgaga agatggaata tatccccgac tgggtcgtgg ttacccggtt
     7981 aaacatctgg tggcgctaat aaatctggca agtcacattc tgttgaatgc tgtgacagtg
     8041 ccttaccgag agagcgaaat tgtggtgttc tagtgcatag tcgccaccat tccggataat
     8101 tctattgcgt tgttttacaa gttcttttac agcgcagatc tgtcgctgac gttgaatcaa
     8161 tagcctggat ttcagaacac aatgcccgtt tgctggttgg tccggcagta gcattaattc
     8221 attatctgaa tgaaatgtgt caatccacag aaaccgctgt caatgacaca ttttgtcgga
     8281 cgttaacacg agaaagtctg gctattccgt aatgccatct tatttcgtaa tggattgaga
     8341 atgctggatt tttaattata ttacgcgcag tattaatcgc gtaaatgttt tgcggtcagc
     8401 aacggtgggg gatccattgt tctgttcaat gaataccatc atacatttga atcaggagtc
     8461 ggtatgatga ttttggtgac ggggggcgca cggagcggga agagtcgcca cgcagaggcg
     8521 cttattgggg actcttcaca ggttctgtat atcgctacct cgcaaatcct tgatgatgag
     8581 atggctgcac ggatagaaca tcatcggcaa ggccgcccgg agcactggcg cacagtggag
     8641 cgctggcaac atcttgatga attaattcat gcagacatta acccgaatga ggttgtgttg
     8701 ctggaatgcg ttaccacaat ggtgactaat ctgttgtttg attatggcgg cgataaagac
     8761 cctgatgaat gggattatca ggcgatggaa caggcgatta atgctgagat tcagtcgttg
     8821 attgctgcct gccaacgttg ccccgcaaag gttgtattag tgactaacga agtgggaatg
     8881 gggattgtgc cggagagtcg tctggcacga cattttcgtg atattgccgg gcgggtaaat
     8941 cagcagttgg ccgctgcggc aaatgaagta tggctggtgg tttcgggtat tggagtaaaa
     9001 atcaaatgag taaattattt tgggcgatgc tctcatttat tacgcgtctg cctgtaccgc
     9061 gtcgctggtc ccagggactg gatttcgagc attattctcg cggtattatt acttttcctt
     9121 taattggatt attgcttggc gcgattagcg ggctggtctt catggtgctg caggcatggt
     9181 gtggcgtacc actggcggca ctgtttagcg tacttgtgct ggcgctgatg accgggggat
     9241 tccacctgga cggcctcgcc gatacctgtg atggcgtatt ttctgcacgt agccgcgatc
     9301 gcatgttgga aatcatgcgt gatagccgtt taggcaccca tggcggtctg gcattgattt
     9361 ttgttgtact ggcaaagatt ctggtattaa gcgagttggc tctgcgtggc gaaccgatcc
     9421 ttgcatcgct ggcggcagca tgtgcggtca gccgcgggac tgccgcatta ttgatgtatc
     9481 gtcatcgtta tgtgcgggaa gaagggcttg gcaatgtgtt cattggcaaa attgatgggc
     9541 gacaaacctg cgtcacactc ggcttagccg ctatttttgc tgccgtatta ttgccgggta
     9601 tgcatggtgt ggctgctatg gtggtgacga tggtggcgat tttcatcctc ggtcagcttc
     9661 tcaaacgtac gctgggcggg caaaccggcg atacgctggg agctgctata gagcttggtg
     9721 aactggtctt cctgctggca ctgctataaa gagacgtgac atgcaaacac ttgccgattt
     9781 actgaatacg atccctgcta tcgatcctgc cgctatgtcg cgtgcacaac ggcatattga
     9841 cgggttactc aaacctgttg gtagcctggg aaggctggag gcgcttgcca tacaactggc
     9901 gggaatgccg gggttgaatg gcataccgca tgtgggcaaa aaagcggtac tggttatgtg
     9961 tgccgatcac ggcgtctggg aggaaggggt cgctatttcc ccaaaagaag tgacagccat
    10021 tcaggctgaa aatatgaccc gcggaacaac cggcgtgtgt gtgctggcag cacaagcggg
    10081 cgctaacgtc cacgtagttg atgttggtat tgatagtgct gagcctatcc ccgggcttat
    10141 caacatgcgt gtcgcacgag gtagcggcaa tattgcttca gctccggcaa tgagtcgccg
    10201 tcaggctgaa aagttgcttt tggacgtcat atgttatacg cgggagctgg caaaaaacgg
    10261 tgtcacgctg tttggtgtag gtgaactggg gatggcaaac acgacaccgg cagcggcaat
    10321 agtcagcaca atcactggcc gggatcctga agaagtggtt gggattggcg caaacctgcc
    10381 gacagataaa ctggctaata aaattgatgt tgtgcgccgg gcgattacgt tgaatcaacc
    10441 aaatccgcag gatggcgttg atgtcctggc aaaagtgggt ggatttgatt tggtcggaat
    10501 ggctggcgtg atgttaggcg ctgcttcctg cggtttaccc gtgttgctgg atggatttct
    10561 ttcttatgct gccgcgctcg cagcctgcca gatgtctcct gcaatcaaac cgtatctcat
    10621 tccttctcac ctgtcggcag aaaaaggggc gcgtattgcg ctgtcgcatt tggggctgga
    10681 gccttatctc aatatggaga tgcgtttagg tgaggggagt ggcgcagctc tggcgatgcc
    10741 catcatcgaa gctgcctgtg cgatatataa caacatgggc gaacttgctg ccagtaatat
    10801 tgttctaccg gggaatacga cttctgattt gaacagttaa aaacgaaaag tctgataaaa
    10861 gctatactta acggatagct ttcgcgactt aggaaaggga catgatgcgt cgtgtaaata
    10921 ttttttgctc atttgctctg ctttttgcca gccaaaatag cctggcggta acttatccat
    10981 tacctccaga gggtagccgt ttagtggggc agtcgcttac tgtaactgtt cctgatcaca
    11041 atactcagcc gctggagact tttgccgcac aatacgggca agggttaagt aatatgctgg
    11101 aagcgaatcc gggcgctgat gtttttttgc cgaagcctgg ctcacaactc accattccgc
    11161 agcaactgat tttgcccgcc actgttcgta aagggattgt ggttaacgtc gctgaaatgc
    11221 gtctttatta ctatccgcca gacagtaata cagtggaagt ctttcctatt ggtatcggcc
    11281 aggctgggcg agaaaccccg cgtaactggg tgactaccgt tgaacgtaaa caagaagcac
    11341 caacctggac gccaacgccg aacacccggc gcgaatatgc gaaacgaggg gagagtttgc
    11401 ccgcatttgt tcctgcgggg cccgataacc ccatggggct gtacgcgatt tatattggca
    11461 ggttgtatgc catccacggt accaatgcca attttggtat tgggctccgt gtaagtcagg
    11521 gctgtattcg tctgcgtaat gacgatatca aatatctgtt tgataatgtt cctgtaggca
    11581 ctcgtgtaca aattattgat cagccagtga aatacacaac ggaaccggat ggttcaaagt
    11641 ggctggaagt tcatgagccg ctgtcgcgca atcgtgcaga atatgagtct gaccgaaaag
    11701 tgccattgcc ggtaacccca tctttgcggg cgtttatcaa cgggcaagaa gttgatgtaa
    11761 atcgcgcaaa tgctgcgttg caacgtcgat cgggaatgcc tgtgcaaatt agttctggtt
    11821 caagacagat gttttaagag cgttgataat tagaaagtaa aaagcctgct cgaaagcagg
    11881 cttttttgaa tttggctcct ctgactggac tcgaaccagt gacatacgga ttaacagtcc
    11941 gccgttctac cgactgaact acagaggaat cgtgtgaacg gggcgcatat taatggcagt
    12001 gcatggtgat gtcaaagggt gaaatgcatt tttttgtttg tttgctgcca atttcaacaa
    12061 agtggtgagc tttcaatctt attgggtaat gaaccatcgt ggtgcatacc ctccttttat
    12121 agggcaggtg aatgcgactg cagataaaaa gagccaatga cgaatctatc ataatctctt
    12181 atttgacagt tggttagctt gcgcatcaac gccaaaatag aactggcaag catcttgcat
    12241 tctggttgta agtaatggcg gcacttgggc cgactcttaa aaaccggagg caacatgaac
    12301 ttcagacgcc tgaaatactt cgtaaaaatt gtagatattg gtagcctgac ccaggctgct
    12361 gaagtattgc atatcgcaca accagcgctc agccagcagg ttgccacact ggaaggtgag
    12421 tttaatcaac aacttttgat tcgcacaaaa cggggcgtta cgccaactga cgccggaaaa
    12481 attctctata cccatgcgcg ggccatttta cgtcagtgtg agcaggccca actggcggtg
    12541 cataacgttg gtcaggcatt atcggggcaa gtctcgattg gctttgcgcc aggaaccgct
    12601 gcgtcatcca tcaccatgcc cttattacag gcggttcgcg ctgaattccc ggaggtcgtt
    12661 atctatcttc atgaaaatag cggtgcagtg cttaacgaaa aactgataaa tcaccaactc
    12721 gatatggcgg tgatttatga gcattcccct gtggctggtg tatccagtca ggctttactg
    12781 aaagaagatc ttttcctggt agggactcaa gattgtccgg ggcaaagcgt tgatgttaat
    12841 gctattgcgc aaatgaacct ctttcttccc agtgattaca gtgctgttag acttcgtgtt
    12901 gatgaggctt tttccctacg gcgactcacg gcaaaagtca ttggtgaaat tgagtctatt
    12961 gccacgctta ccgcagcgat tgccagcggc atgggcgttg cagtattacc cgaatcggcc
    13021 gcgcgttcgt tatgtggcgc agtaaacggg tggatgtcac gcattaccac accttccatg
    13081 agtctctctt tgtcattaaa tttacccgcc agagcgaact tatcaccaca ggcacaggca
    13141 gtgaaagagt tgttaatgtc agtgatgagt tctccagtga tggaaaaaag gcagtggcaa
    13201 ttggtgagct aagcgttata tcgggatgga ataagatgcg ggtttttatt atttgttatg
    13261 ccgggcatta gactttaaca ataacggaaa atctgaactg cccggagttt accgtgaatt
    13321 tccagcaact aaagataatc cgcgaggctg cacgtcagga ttacaacctg acagaggttg
    13381 cgaatatgct ttttacctca cagtccggcg tcagccgtca tattcgggaa ctggaggatg
    13441 aacttggcat cgaaatattt gttcgacgag gtaagcgact gctgggcatg actgaaccgg
    13501 gcaaagcatt actggtcatt gcagaacgta ttctgaatga agccagtaat gttcgtcggc
    13561 ttgcagacct gtttaccaac gatacgtctg gcgttctcac tattgcaacg acgcatactc
    13621 aggcacgtta tagtttgcca gaggtcatta aatcttttcg cgaacttttc ccggaggttc
    13681 ggctcgagct aatccagggg acgccacagg aaattgcgac gttgttgcat aatggtgaag
    13741 ctgatattgg tatcgccagc gagcgtttga gtaatgaccc gcagctcgtc gccttcccgt
    13801 ggtttcgttg gcaccatagt ttgcttgttc cactcgatca tcccttgacg caaattacac
    13861 cattgacgct ggaatcaata gcgaagtggc cgttaatcac ttaccgacag gggattacgg
    13921 ggcgctcacg tattgatgac gcatttgccc gcaaaggttt gctggcagat attgtattaa
    13981 gtgcgcagga ttctgatgtc attaaaacct atgttgctct tgggcttggg atcggaatag
    14041 ttgccgaaca atccagcggt gaacaagagg aaaagaattt aatccgcctg gatacgcggc
    14101 atctttttga tgctaatact gtctggttgg gactgaagcg aggacaactt cagcgtaact
    14161 atgtctggcg ctttctggaa ctttgtaatg cagggctttc agttgaggat atcaagcggc
    14221 aggtgatgga aaacagtgaa gaggaaattg attatcagat atagcaaaaa gcccgctata
    14281 aaagcgggct tttcagaaat ttggctcctc tgactggact cgaaccagtg acatacggat
    14341 taacagtccg ccgttctacc gactgaacta cagaggaatc gctgttgagg cacatcttaa
    14401 cggcgaaaaa tcttttgtca aacccgattt ttaattttcc caactgtttg ccgtttctat
    14461 caacagtttg ttgcatttgc agacaatttg ttggcgaaaa tctttgcagc tttgctgatt
    14521 ctccctcatt atttgaaatg tggtttcact ttctgcaatt aaggtcggct ttgaatatct
    14581 cctctgcttt acgccaggtt gttcacggca ctcgctggca cgctaaacgc aagagctaca
    14641 aagtgttgtt ctggcgcgag ataaccccgc ttgctgttcc tatcttcatg gagaatgcct
    14701 gtgtcctgtt gatgggggtt ctgagcactt ttctggtcag ctggctggga aaagatgcga
    14761 tggccggcgt gggattggcg gacagcttca atatggtcat tatggctttt tttgctgcta
    14821 tcgatcttgg tactactgtc gttgtggcat ttagtctcgg taagcgggat cgacgacgag
    14881 cgagggtggc gacgcggcag tcattggtga tcatgacgtt gtttgccgta ctgttggcaa
    14941 cgcttattca tcattttggc gagcaaatta ttgatttcgt cgcgggtgat gccacgacag
    15001 aagttaaagc actggcgttg acttatctgg agctgacggt actcagttat ccagcagctg
    15061 ccatcacttt gattggtagc ggggcacttc gtggtgcagg gaatacgaaa ataccgctac
    15121 tgattaacgg tagcctgaat attcttaata ttattattag cggcatattg atttacggcc
    15181 ttttctcctg gccgggactg ggatttgtcg gggctgggtt ggggttaacc atttctcgtt
    15241 atattggcgc agtggcaatt ttgtgggtgc tggcgattgg ttttaatcct gcgctaagga
    15301 tttcgttaaa gagctatttt aaaccgctga attttagcat tatctgggaa gtcatgggga
    15361 ttggtattcc cgcgagtgtc gaatcagtgt tatttaccag tggtcggtta ttaacccaaa
    15421 tgttcgttgc cgggatgggg accagtgtta ttgccggaaa ttttatcgcg ttttcaattg
    15481 cggctcttat caacttaccc ggaagtgcgc tcggctctgc ttctacgatc attacaggcc
    15541 gaaggttggg tgtaggacag atagcgcaag cagagattca gttgcggcat gtgttctggc
    15601 tttccactct tggattaacg gccatcgcct ggctaacggc tccctttgcc ggggttatgg
    15661 catcgtttta cacccaggat ccacaggtta aacatgtcgt tgtgattctg atttggctaa
    15721 atgctttatt tatgcctatt tggtctgcct catgggtgct acccgctgga tttaagggtg
    15781 ctcgcgatgc acgttacgcc atgtgggttt ctatgttgag catgtggggt tgccgggttg
    15841 tagtcggtta tgtgctggga atcatgcttg gctggggtgt ggttggtgtc tggatgggaa
    15901 tgtttgccga ctgggctgtg cgggccgtgc tgttttactg gcgaatggtt actggacgtt
    15961 ggctgtggaa atatcctcga cctgagccgc aaaagtgtga aaaaaagcca gttgtgtcgg
    16021 aataaacgac aaagtgcaga ttatttcagc aaacgatttc aaatttaaaa aacaggcttt
    16081 gacattgtgg atgagcgtcg ctaatattcg cctcgttctc acgattcctc tgtagttcag
//
LOCUS       DAPHJE010000099        16057 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPHJE010000099 DAPHJE010000000
VERSION     DAPHJE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.100"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1107
                     /locus_tag="RH222_003314"
     CDS             55..1107
                     /locus_tag="RH222_003314"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV3798285.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1183..2616
                     /locus_tag="RH222_003315"
     CDS             1183..2616
                     /locus_tag="RH222_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV3798286.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2799..5060
                     /locus_tag="RH222_003316"
     CDS             2799..5060
                     /locus_tag="RH222_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV3798287.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5294..6577)
                     /locus_tag="RH222_003317"
     CDS             complement(5294..6577)
                     /locus_tag="RH222_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV3798288.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6712..7782)
                     /locus_tag="RH222_003318"
     CDS             complement(6712..7782)
                     /locus_tag="RH222_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3798289.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGPKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASSKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(7760..7978)
                     /locus_tag="RH222_003319"
     CDS             complement(7760..7978)
                     /locus_tag="RH222_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDV3798290.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(8018..8185)
                     /locus_tag="RH222_003320"
     CDS             complement(8018..8185)
                     /locus_tag="RH222_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798291.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(8280..8561)
                     /locus_tag="RH222_003321"
                     /pseudo
     CDS             complement(8280..8561)
                     /locus_tag="RH222_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231890.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            complement(8561..8788)
                     /locus_tag="RH222_003322"
     CDS             complement(8561..8788)
                     /locus_tag="RH222_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798292.1"
                     /translation="MSKQEFLQKLASLASECHALACQLDIGDERTEMFEIYNVLRSLG
                     RSGYASQISKGTNPLLWAVDDNDDDWDEDDE"
     gene            complement(8785..9531)
                     /locus_tag="RH222_003323"
     CDS             complement(8785..9531)
                     /locus_tag="RH222_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV3798293.1"
                     /translation="MSKIDYQALREKAEKATCGVWSLEYGEEIFDAGDALIHREVVGY
                     LPICRIEGAHPEGGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEEIALMVGKLRVDLEAAENNLIDSECHVDELEEALRDKQALLEASEKRIAELEAEPV
                     SQTYKLNKLAGNSPVTPDGWISCSERMPEDTKMLLAFSQGEIVAAYWNWVVNPVDYKK
                     YRAFTYLSGNILDDVTHWMPLPEPPQEVNP"
     gene            complement(9528..10010)
                     /locus_tag="RH222_003324"
     CDS             complement(9528..10010)
                     /locus_tag="RH222_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798294.1"
                     /translation="MKQMSLIEMDGFLKGKCIPRDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAAHPQPFGPEMMKALDAYEKHQDEVPETGMLDAFFILRDSIRVETPAT
                     DAFLAEVRAQGVEMYADNLDNGADDAERGGFDYAVKFLRSEASSVRLFANQLRKGGSQ
                     "
     gene            complement(10007..10171)
                     /locus_tag="RH222_003325"
     CDS             complement(10007..10171)
                     /locus_tag="RH222_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDV3798295.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRSGKK"
     gene            complement(10182..10478)
                     /locus_tag="RH222_003326"
     CDS             complement(10182..10478)
                     /locus_tag="RH222_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDV3798296.1"
                     /translation="MPAPLYGADDPRRCSGNSISEVLDKFRKNYDLIMSLPQETEEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEYIDPRLRNYRSRYGAISND"
     gene            complement(10502..10885)
                     /locus_tag="RH222_003327"
     CDS             complement(10502..10885)
                     /locus_tag="RH222_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798297.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(10885..11490)
                     /locus_tag="RH222_003328"
     CDS             complement(10885..11490)
                     /locus_tag="RH222_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDV3798298.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAVAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(11747..11917)
                     /locus_tag="RH222_003329"
     CDS             complement(11747..11917)
                     /locus_tag="RH222_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798299.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRMKSLLE
                     KLWDILKQQGRP"
     gene            complement(11993..12304)
                     /locus_tag="RH222_003330"
     CDS             complement(11993..12304)
                     /locus_tag="RH222_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HDV3798300.1"
                     /translation="MKLRVWHIPQVPMKPFIVEVASVEEGVRLMDALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEMGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
     gene            complement(12449..12775)
                     /locus_tag="RH222_003331"
     CDS             complement(12449..12775)
                     /locus_tag="RH222_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798301.1"
                     /translation="MKVLMVYENVPESTEIYIFDANEDEVNDLKLSHGNYTNANCDES
                     IEKALSRVLVRISDPEHCDDDWLSYCGAVKTDAGKWSKSKVDNSTPIIMKDSDIEMVI
                     ITGMIM"
     gene            complement(12816..13016)
                     /locus_tag="RH222_003332"
     CDS             complement(12816..13016)
                     /locus_tag="RH222_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HDV3798302.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIAREIGKQNNKAA"
     gene            complement(13095..13421)
                     /locus_tag="RH222_003333"
     CDS             complement(13095..13421)
                     /locus_tag="RH222_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HDV3798303.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(13725..14129)
                     /locus_tag="RH222_003334"
     CDS             complement(13725..14129)
                     /locus_tag="RH222_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798304.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGAMNGDRYDFTKDST
                     EDSVFHITINGDKSSVYESITGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNH"
     gene            complement(14126..14758)
                     /locus_tag="RH222_003335"
     CDS             complement(14126..14758)
                     /locus_tag="RH222_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDV3798305.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            14862..15077
                     /locus_tag="RH222_003336"
     CDS             14862..15077
                     /locus_tag="RH222_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3798306.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            15197..15475
                     /locus_tag="RH222_003337"
     CDS             15197..15475
                     /locus_tag="RH222_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDV3798307.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMGMLLAVLEWGVEDEELAELAKKVARMLTKEKAPKNGEFFEA"
     gene            15510..15656
                     /locus_tag="RH222_003338"
     CDS             15510..15656
                     /locus_tag="RH222_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HDV3798308.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            15649..>16057
                     /locus_tag="RH222_003339"
     CDS             15649..>16057
                     /locus_tag="RH222_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV3798309.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTK"
BASE COUNT         3911 a         3864 c         3721 g         4561 t
ORIGIN      
        1 tccttaatgt aaccatatcg caataagtta tgtttttaaa ttgagggcat tattatgaaa
       61 aaaataccct gcgtgatgat gcgaggtgga acctcgaggg gcgcgttcct gttagcggaa
      121 catttacccg aagatcaaac gcagcgcgat aaaatattga tggcaattat gggttccggt
      181 aacgatctgg aaattgacgg tattggcggc ggtaatccac tgaccagtaa agtcgccatt
      241 attagccgtt ccagcgatcc gcgtgctgat gttgattatc tgtttgctca agttatcgtc
      301 catgagcaac gtgtcgatac cacgcctaac tgcggcaata tgctttctgg cgttggggca
      361 ttcgccattg aaaatggttt gattgcagcg acatcaccgg tcacccgtgt gcgtatccgc
      421 aacgtcaata cgggtacgtt catcgaagct gatgtgcaaa cgccaaatgg tgttgtcgag
      481 tacgagggta gtgccagaat tgacggcgta ccgggtactg ccgcaccggt tgcgctcact
      541 ttcctgaatg ccgctggaac caaaaccgga aaagttttcc cgactgataa tcagattgat
      601 tattttgacg atgtcccggt gacctgtatc gatatggcga tgccagtcgt cattattccg
      661 gctgaatatc tgggaaaaac aggttatgaa ttaccggcgg aactggatgc cgacaaagca
      721 ttattagccc gcattgaatc tatccgtcta caagcgggta aagcaatggg cttaggtgat
      781 gtcagtaata tggttatccc taaacctgtg cttatttctc cagcgcagaa aggcggggca
      841 attaatgtgc gttattttat gccgcattct tgccatcgcg cgctggcgat aaccggtgct
      901 attgctattt ccagtagttg tgcattggaa ggcaccgtca cccgacaaat cgtcccttct
      961 gtaggatacg gcaatatcaa tattgaacac cccagtggtg cgctcgacgt tcatttaagt
     1021 aatgaaggtc aggatgccac gacgttacgc gcatctgtta ttcggacgac cagaaaaata
     1081 ttttccggtg aagtttatct tccctgaaaa aattcgttgt caagataagg ataatcaata
     1141 aaggacttct gtatgagtca tacagaaaga acaggatttt aaatgaataa gaaatcgtta
     1201 tggaagctaa ttctgatatt agcgatccca tgtattattg gttttatgcc agctccggca
     1261 ggattaagcg aactggcgtg ggtgcttttt ggtatttacc tggcggccat tgtggggctg
     1321 gttatcaagc ctttcccgga acctgtcgta ctgttaattg ccgttgctgc ctccatggtg
     1381 gtggtcggta acttatccga cggtgcgttt aagaccaccg ccgtattaag cggttactct
     1441 tcaggtacta cctggctggt gttctcggcg tttaccttaa gcgccgcatt tgtgaccacc
     1501 ggtttaggta aacgtattgc ctatctgctg attggtaaaa tcggtaacac cacgctgggt
     1561 ctgggttacg ttacggtatt cctcgatctg gtactggctc cggcaacacc gtctaacacc
     1621 gcgcgtgcgg gcggtattgt gttaccgatc atcaacagcg tggcggtggc tttggggtcc
     1681 gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga tgatgtccat ttacatggtc
     1741 accaaaacca ccagctatat gttctttacc gcaatggcgg ggaacattct ggcgctgaaa
     1801 atgatcaacg acattctgca cctgcaaatt agctggggtg gatgggcact ggccgccgga
     1861 ttgccgggca tcattatgct gctggtcacc ccactggtga tttataccat gtatccgcca
     1921 gaaattaaga aggtggataa taaaaccatc gctaaagcgg gccttgccga actgggaccg
     1981 atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc tggcgctact gggctggatt
     2041 ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa tcgttgttat ggcgaccatg
     2101 ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa ataaaggcgg ctggaatacc
     2161 ttaatctggt acggcggtat tatcggatta agctccttat tatcgaaagt taaattcttc
     2221 gaatggttag ctgaagtctt taaaaataac ctggcatttg atggtcacgg taacgttgct
     2281 ttcttcgtta ttattttcct cagcattatc gtgcgttatt tcttcgcttc cggtagtgcc
     2341 tatatcgttg ctatgttacc ggtatttgcc atgctggcga acgtctccgg cgcaccgtta
     2401 atgttaaccg cgctggcact gttgttctcc aactcctatg gcggcatggt tactcactat
     2461 ggcggcgcgg caggtccggt catctttggc gtgggttata acgatattaa atcctggtgg
     2521 ttggtcggtg cggtactgac gatattaacc ttcctggtgc atatcaccct cggcgtgtgg
     2581 tggtggaata tgctgatcgg ctggaacatg ctgtaaatat tctcgtcata cttcaagttg
     2641 catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct cctggggatt
     2701 cactcgcttg tcgccttcct gcaactcgaa ttatttagag tatatccatt tattatcttt
     2761 ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta tctgaaaaag
     2821 gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc ggcgaaatta
     2881 aaaaagaaga agccaaaaaa ggcactattg cctggtctat tctctcttcg cataatacgt
     2941 ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac gatattacct
     3001 ttgttggtat tgtacagacc gctaaagcgt ccggtatgga acgtttcccg ctgccgtatg
     3061 tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg cactattaac ggtgatgacc
     3121 atgtttttgg tttatcggcg gcccagcgtt atggcggtat ttttgtgcct ccgcatattg
     3181 cggtcatcca tcaatatatg cgtgagatga tggcaggcgg cggcaaaatg atcctcgggt
     3241 cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag ggcggcggtg
     3301 agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc gtggttgcgg
     3361 tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg ctggctatca
     3421 ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc gttggaccgg
     3481 gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc acggaaacca
     3541 cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg gcgctgcacg
     3601 gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac gatggctgca
     3661 tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac ccgagcaacg
     3721 tgtataaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag attgaaattg
     3781 agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa gtggaaaatg
     3841 gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac tacgaaaacg
     3901 tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc ttctcgctgg
     3961 cagtttaccc gtcatcacag ccggtgttta tggatctcgc caaaaaaggt gtggtagcag
     4021 atttgattgg cgcaggcgca atcatcagaa ccgcgttctg cggcccatgc tttggcgcgg
     4081 gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac ttcccgaacc
     4141 gcgaaggctc taagccagct aatgggcaga tgtcagcggt ggcgttgatg gacgctcgtt
     4201 ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa cttgattgct
     4261 gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt gtttatcagg
     4321 gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt aaagactggc
     4381 cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag atcctcgacg
     4441 aagtgaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat cgttcaaatc
     4501 cgattggtct ggcggagttt accctgtcac gccgcgatcc cggttatgtt ggcagaagta
     4561 aagcgactgc tgagctggaa aatcagcgtc tggcggggaa tgtcagcgag ctgacagagg
     4621 tgtttgcgcg cattaagcag attgctggtc aggagcatat tgatccgctg caaactgaaa
     4681 ttggcagcat ggtatatgcg gtgaaaccag gcgatggttc tgcgcgtgaa caggcggcga
     4741 gctgccagcg tgtgattggc ggtctggcga atattgccga agagtacgcg actaaacgct
     4801 accgttctaa cgtcatcaac tgggggatgt taccgctgca gatggcggaa gtgccaacct
     4861 ttgaagtggg ggattacatt tacatccctg gcattaaagc ggcgctggat aatccgggta
     4921 cgacgtttaa aggttatgtg atccatgaag atgcgccggt aacggaaatt acgctctata
     4981 tggaaagtct cactgctgaa gagcgcgaga ttatcaaggc gggtagtttg attaacttca
     5041 ataaaaaccg tcagatgtaa aaagcgccat gtgaatgtag gtcgcattcg gcacttattg
     5101 tcggatgcga tgcttgcgca tcttatccga cctacgaatc gcatcgaatc tgtaggccag
     5161 ataaggcatt ttcgcagcat ccggcactta ttgtcggatg cgatgcttgc gcatcttatc
     5221 cgacctacaa atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa
     5281 tagttaattg ctcttacttc ttcgcctctg caaccacttt actacccacg ccgcggttat
     5341 tgtattccca catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat
     5401 catctacgct gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg
     5461 gtttagccgt gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg
     5521 tattggcatc aacatccagc gagcggccca gttgcgccac accatcaccg aaagcattga
     5581 aacggctgtt tacggcgagg aaaccgtagt aaatgttgga cagcgtagcc ggtgcaaaca
     5641 catacgcttc ttgctgagta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca
     5701 ctgcgccgcg accagaaacg atatccacat ccccttcaat gtagctgttg gtcaccagcg
     5761 tacgcggctg acgattcgtt tccagacggt tctgcacacc gctgttggtg acaaagaagg
     5821 tgttctgacg accgagaatg ttaacgttgt taatctgtac ctggtcacca tcagtacgca
     5881 gtgccaccgc cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca
     5941 gattttgcag ttgcaggcca ttgttttgtg accagaagac cgcagagcag agaacaccga
     6001 tactgtcgct gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca
     6061 tatatttgcc gcgcgggttg acgtcgtgac gccagtcggc agggctcatg ccaccatcaa
     6121 gggaaagccc aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtaattccac
     6181 ccggagcggc agggacatat accgttccct gatactcacc aggcatcacg gcaatatact
     6241 ggcgcttgtt ggtacgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg
     6301 ttacaccttg agtgcccgcc gggccgacaa caaagtcagg ttgcgcaggc agggtaatcg
     6361 gggaaggatt ccacgctgct gcacctggtg tcagggatgc aaaatagtgt tgagcatcga
     6421 aattctgcgc ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcggtttgat
     6481 cagaaggacg ttgatcgggc ggggttgagc tacaggcggt cagcgtcacg ccaaaagcca
     6541 atgccagcgc cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata
     6601 gaaaaatgaa tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt
     6661 gcttatcaat ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat
     6721 ttcaattttg tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga
     6781 cttatgcccg agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc
     6841 agacaaactg cgcaactcgt gaaaggtagg cggatcccct tcgaaggaaa gacctgatgc
     6901 ttttcgtgcg cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt
     6961 agatgcaatt atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat
     7021 tgatatcccg agagcatcaa catgcaatgt tgttgggatg gcaattttta cgcctgtttt
     7081 gctttgctcg acataaagat atccatctac gatatcagac cacttcattt cgcataaatc
     7141 accaactcgc tgcccggtaa caacagccag ttccattgca agtcttagcc aacatggtga
     7201 tgattctgct gcttgataaa tttttaggta ttcgtcagct gtaagtcttg atctccttac
     7261 ctctgatttt gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat
     7321 tgcctctcgg aatgcatcgc tcagtgttga tctgattaac ttggatgacg ccgccttgcc
     7381 ctcgtctatg tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg
     7441 agcatcaggc agacccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt
     7501 ctgcttgatt cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt
     7561 aacagaatta tcactgttga ttcttgctgt cagaggcttg tgtttgggtc ctgagaataa
     7621 ctcaatgttg gcctgtatag cttcagtgat tgctatcctt ctgtctctgc ctaatccgaa
     7681 ctctttaccc gtccttgggt ccctgtagca gtaatatcca ttgtttctta tataaagatt
     7741 agggggtaaa tcccggcgct catgacttcg ccttcttccc atttctgatc ctcttcaaaa
     7801 ggctacctgt tactggtcga tttaagtcaa cctttaccgc tgattcgtgg aacagatact
     7861 ctcttccgtc cttaaccgga ggagggaata tcctgcattc gcgtacccat cgacgaactg
     7921 tttcaaggct tcttgggcgt cgctggcgag cgttccactc ctgaagtgtc aagtacatcg
     7981 caaagtctcc gcaattacac gcaagaaaaa accgccatca ggcggcttgg tgttctttca
     8041 gttcttcaat tcgaatattg gttacgtctg catgtgctat ctgcgcccat atcatccagt
     8101 ggttatagca gtcgttgatg tcctctgctt cgataaccct gttgaatggt tctccattcc
     8161 attcacctgt gactcggaag tgcatttatc atctccataa aacaaaactc gccgtagcga
     8221 gttcagataa aagaaatccc cgcgagtgcg aggattgtta gttgcgctct gctgctgatt
     8281 tagccatcaa tattcacctt tatcgcgtac acctttaccg gtttatcgcc gaagtgggga
     8341 tgtgtgattg tctttatttc atatccgtca tacggaacat caattctgcg gctggaatcg
     8401 tcgcgcttcg gatatccctt tgtgataatc aggcggtcat actcccggaa cataattcac
     8461 ttattccagt agtcattaca caggcgatac tcttccgttt tctctccgcg aatcatggca
     8521 tcgaagtatt cacctttaac ggcaagttgc aggttagcca tcactcatca tcctcatccc
     8581 aatcatcatc gttgtcgtca accgcccata acagtgggtt ggtacctttg cttatctggc
     8641 ttgcatatcc gctacgtcca aggcttctca acacgttgta tatttcaaac atctcagtgc
     8701 gttcatcgcc aatgtctagc tggcaggcca gtgcgtgaca ctctgaagcg agcgaggcta
     8761 acttttggag gaattcttgt ttactcacgg attcacctcc tgcggtggtt ctggtagcgg
     8821 catccagtga gttacgtcat ccaagatatt tcctgataaa tacgtgaaag ctctatattt
     8881 tttgtaatca actggattta caacccagtt ccaatatgcg gccacgattt caccttgact
     8941 aaatgccagt aacattttgg tgtcttccgg cattcgctca ctacagctta tccaaccatc
     9001 aggagttacc ggagagttgc cagccagctt gttcaacttg taagtctggc ttacaggttc
     9061 ggcttccagt tctgctatgc gcttctctga tgcttcaagt aacgcctgct tatcgcgtag
     9121 cgcttcttcc agttcatcaa catggcattc actatcaata aggttgttct ctgcggcttc
     9181 aagatcaacg cgcagctttc ctaccattag cgcaatttcc tcgttctcct ggtcgcggcg
     9241 tttgatgtat tgctggtttc tttcccgttc atccagcagc gccaagacgg tagccggatt
     9301 ggctgcggcg atgaattcag cattggcctg ctgttccatt tggaaatctt catcgaaacc
     9361 gccttcagga tgcgctcctt caattctgca aatgggaaga tatccaacaa cttcacgatg
     9421 aattagtgca tcaccagcat caaatatctc ctctccatat tcgagcgacc acaccccaca
     9481 cgttgctttc tctgcctttt cacgcagtgc ctgatagtca atcttgctca ctggctgcct
     9541 cctttgcgaa gctggttggc gaacaaacgt acactagacg cttcactgcg tagaaactta
     9601 acggcataat caaaaccacc tcgttctgcg tcgtctgctc cgttgtcgag gttatctgcg
     9661 tacatctcta ccccctgcgc ccgtacttca gccaggaaag catcggtggc tggtgtttca
     9721 acacggatgc tgtcgcgcaa gatgaaaaat gcatcaagca ttccagtctc cggcacttca
     9781 tcctgatgct tctcatacgc atcaagagcc ttcatcatct caggtccgaa tggttgaggg
     9841 tgcgcagcct tcagccccgc attctccgcc gccagcgccg cgcacttggc ctccgcttca
     9901 gcaaatttac gcaccagata ttcagcgttt gtttcgttaa cctttaaatc tcgggggatg
     9961 catttacctt tcagaaaacc atccatctca attagtgaca tttgtttcat tttttcccac
    10021 tccgccacat cgcattcaga tatttgtttt gattcactga tggaaaagaa tttctcttaa
    10081 gcaattcctc tctcgatggc attggcttta cgcgttggcg aataatcatt tctgccggaa
    10141 gaatgccggg attgtatgca agtcctctca tggtaaattc ctcagtcatt actgatagcg
    10201 ccatagcgtg agcggtaatt acgcaggcgc gggtcgatat attcagggaa gtgggtatat
    10261 gtggctttgc ggaatggtcg gattgatgtc tggtaaattc gctcgcgctc ttctttctct
    10321 gcaagccata tacaatgacg aaattccttt tcctcttccg tttcctgcgg tagcgacatt
    10381 atcaggtcgt agttttttct gaatttatcc agcacctccg atatggaatt gccggaacaa
    10441 cggcgcgggt catccgcacc atacagaggc gctggcataa tggaatcctt atgttgctac
    10501 tttagaaggg aattgaatcg tcgtattcag gatgattttg atgattgcta ctttgctgct
    10561 gttggctgtt tcctgaagtt gcaaatccaa tctttgcatt cagtaattca agagtgattg
    10621 attgaccatt ttgcccctga taaacatcaa ccctgatgtt ttctccggta atttccacaa
    10681 tgccaccttc aacaagaaca ctacggtagt aatccgcttg cgctcccggc ttggcaaata
    10741 caacggcgct gtagtttgtc cattctttct tttttgtctg gcgatcgtaa tactgaacgc
    10801 cagcacggat gttgaatccg atattttccc cggcctgaaa ctctcttgcg ggcttgttta
    10861 gtcttacagt aatcgaatgt gccattaagc agccgctcct tctaattcgt ctcgtctgat
    10921 gttgtaaacg tcctgcgctt tgtgctgctc cggtgtgcct tcgagcatct tccacgcttt
    10981 ggcgaacgcc tgtttaagct cttccacggt gtttttctgc attgctgcgt cagtgaatgc
    11041 ttttagaacc tgttcaggtg taggtgatgg ttttgattgc tttgctactg cgttctgctg
    11101 atgtttatgc tcgtctgtat ctgcatcttt cgcatcatca atgccgaaca aaccattgag
    11161 gcaatacttg cgtgcataag agcttgtagc gcccgtaact tgtgcagaat ccattccttt
    11221 cttgctttct tcctctcgtg caagagcggt tgccgtatga ctgttttcgc catcggtaat
    11281 agttgccgtg gctttcacgt aataccgatc accaatcaac acaacttcat cgctgattga
    11341 taaaaacaga ccattcagta acggcttaac gccttcaaga atatcttcgc agcttctgta
    11401 tttatatttg ccgaatgagt tgtactgatt ctttggcgcg ttcagattct cctgaatagc
    11461 tgccagtctt gcgtaaaatt ctttgctcat atgattgttc tcagaatgga cacggcccaa
    11521 ggaaataacg ctgatttaat acttcgactc gggacaagtt aaggcatacc cgcattcctt
    11581 cgcggtcgcc attatggcga taccagagag ctttctgcgt gtacatgcgt ctctgtaact
    11641 tgctctcctt cactgtggtt gcaagtgaca tgaatatctc cttcgttacc gattaattct
    11701 ttcatctgaa gaatgaattc ttcgtctgac cagttatctg taaaactcat gggcggcctt
    11761 gttgtttcaa aatatcccaa agcttttcga gcaagctttt cattcttggt tgtttaaagt
    11821 ctgctccggt taaaatattt tttcgtgaat gctgtaccga taaaatcggg ttgaaagggc
    11881 gaaccgatgc cgcccctgca atagcgaact gttgcatagg atgctccttc tgtttgattg
    11941 cataacgaaa acgcctcgag tgaagcgtta ttggtatgcg gtaaagccgc gcttaggcgg
    12001 ctgatgtttc ttctttcagg ctttcgagat atttacgtgg gtcgtcgtaa cattggcatt
    12061 cgctgtacca atccacccag cgatcagtaa gccccatctc tgataaatct tcatcggtaa
    12121 ggctctcatc ccacatctca aggccgttag cattgcagta atcaggcttg atgttgttgt
    12181 catactgaaa ggcgtcataa tcagccagtg cgtccatcag gcgaacaccc tcttcaacac
    12241 ttgccacttc tacaatgaac ggcttcatag gtacttgcgg gatatgccag acacgtaatt
    12301 tcatatttcc tccgtcaaaa aaatgccctc acactggagg gcaaagaaga tttccaataa
    12361 tcagaacaag tcggctcctg tttagttacg agcgacattg ctccgtgtat tcactcgttg
    12421 gaatgaatac acagtgctta ttcgcagcct acataatcat tccggttatt attaccattt
    12481 caatatcgct atctttcata atgattggag ttgagttatc aactttactt ttactccatt
    12541 ttcctgcatc agtttttacc gctccacaat aagaaagcca atcatcatca caatgttctg
    12601 gatcactaat tctgacaaga acacgtgata gtgctttttc gatactttca tcacaattag
    12661 catttgtgta attgccgtga gacaatttca aatcgttaac ttcatcttca ttggcatcaa
    12721 aaatatagat ttcagtcgac tctggaacat tttcataaac cattaaaact ttcattttta
    12781 ccccatattg tttatgccaa aaataaaggc caccattagg cagccttgtt gttctgttta
    12841 ccaatttctc tggcaatcat tgccgtcgtt cgtattgccc atttatcgac atatttccca
    12901 tcttccatca caggaaacat ttcttcaggc ttaaccatgc attccgattg cagcttgcat
    12961 ccattgcatc gcttgaattg tccacaccat tgatttttat caatagtcgt agtcataagg
    13021 atagtcctgg tattgctcca tcacatcctg cggatgctct tcgaactctt caaattcttc
    13081 ttccatatct cacctcaaat aagtggtttg ctgcctaatt tcattttctg gcgaccaaca
    13141 caagtcacac ccatttcact gcgtggcttg ctgtaccatg tgcgctgatt cttgcgctca
    13201 atacgttgca ggttgctttc aatctgttcg tggtattcag ccagcactgt aaggtctatt
    13261 ggattcagtg cgctttctac tcgtgatttc ggtttgcgat tcagcgagag aatagggcgg
    13321 ttaactggtt ttgcgcttac accaaccaac aggggatttg ctgctttcca ttgagcctgt
    13381 ttctctgcgc gacgttcgcg gcggcgtgtt tgtgcatcca tctggattct cctgtcagtt
    13441 agctttgagt aacgcgccgt gatgcttatc tccacggttg ctgtcttgca gctgcatttc
    13501 gcgctactca aagccttctg ctttgaatgc tgcccttctt cagggctaaa tttttaagag
    13561 catcaccttc atggtggtta gtgcgtcctg ctgatggctt aaaattacaa gaaagattgt
    13621 atgttgtaaa caagaaatat tgtaaatgga ggtgcaaaaa acaaactcca ttgtttttaa
    13681 acggaaaata gtttgttttt gggttactga gattgaggtg gggattagtg gttgcaggtt
    13741 ccgactacat caccaacaaa ggatttggtt gatgtaagtt gctgcatgcc cgggatattc
    13801 attactttgg agtaaagagc ttttttgtct gtagtgattg accaagtttc aacggttatt
    13861 ccgccaccag actggtattc tcctaccata gtgttcgatg acaaagccgt gtatttcatc
    13921 tctggataga cgccagtgat tgattcataa accgatgatt tatcaccatt aattgttatg
    13981 tggaaaacgg aatcttccgt gctgtctttt gtaaagtcgt aacgatcgcc attcattgcc
    14041 ccgtacccgt gcaggtttgt gacaatccag cattcagaat tggcgctggt agttaatagt
    14101 attgagagta gcgccgcaat cctgatcata cgaattttac cctcgcttcc acgacaacac
    14161 cgataatctt gcagttcccg ttgatgggag tcataggcca tgaaggattc aggcctttca
    14221 ggtacttctg accgccatct ataaccagtt tcttgaatgt tgcttcgttc gcgtcagtca
    14281 gttttgctac aacaaggctt ccattcactg gctcgcgtcc agtatctact aacaccatat
    14341 gaccttcagg gatgctttga cctacaggtg aggtcatgga atcaccttca accttcagcc
    14401 agaaaccatc gcctaataag ttaacgtcac tgtcatacca ttcatcaatg tccttgatat
    14461 cgtagggttc acaagcttca caccacgaac cagctctaac catgctaatc aatggatatt
    14521 tccctttggg ctcaacgtgc ccaacaaatc taacattcga atcagaggtg ccattgagca
    14581 gccagtcaac acttacgcca agagctgacg caagttctgg taaaaagcgt ggtcgcttag
    14641 ttttaccgtt ttcgagctgc tctatagact gctgggtagt ccccaccttt tgagcaagtt
    14701 cagcctggtt aagtccaagc tgaattcttt tgctttttac cctggaagaa atactcataa
    14761 gctacctctg ttatttacct ccaatcttca caagaaaaac tgtatttgac aaacaatata
    14821 cattgtatga aaatacaaga aagtttgttg atggaggcga tatgcaaact ctttctgaac
    14881 gcctcaagaa gaggcgaatt gcgttaaaaa tgacgcaaac cgaactggca accaaagccg
    14941 gtgttaaaca gcaatcaatt caactgattg aagctggagt aaccaagcga ccgcgcttct
    15001 tgtttgagat tgctatggcg cttaactgtg atccggtttg gttacagtac ggaactaaac
    15061 gcggtaaagc cgcttaagac attcccgctc ttacacatcc cagccctgaa aaagggcatc
    15121 aaattaaacc acacctatgg tgtatgcatt tatttgcata cattcaatca attgttatct
    15181 aaggaaatac ttacatatgc aacttacaag tactcgcaag aaagcgaatg caattacaag
    15241 caacatcctg aatcgaattg ctgtacgtgg ccagcgaaag gttgccgacg cgttagggat
    15301 taatgaatcg caaatttcgc gatggaaaga cagcttcatc ccaaaaatgg gaatgcttct
    15361 ggctgttctt gaatggggtg ttgaagacga ggagttggcg gaactggcta agaaagtagc
    15421 cagaatgctg acaaaagaaa aagccccgaa gaacggcgaa ttcttcgagg cctgatgtag
    15481 aaagactgga tcaatccaca ggagtcatta tgacaaaaca actcagtcct taccaggaca
    15541 aaattcacaa acacatacta cgtgatcgct tcctgtccag cttcaagcag cctggtcgat
    15601 tccgggctga gttggaaaaa gtgaagctga tgcagaagga gaaaggtcat gagtaatctt
    15661 gcaaccgtaa cacatttaag gccttcacaa cggcctgtgg agcgtcgtgt ggcagaagtt
    15721 gaagatggtt atacccgtct tgcaaatgcc ctgtatgaag agcttatcgg cgcagattta
    15781 acgaaaaatc agagcaaggt tgcccacgcc atatgccgta aaacatacgg ctacggtaaa
    15841 aagatggatc gcatctctga tagtcagtta gctcaaatta ccaggctgcc aagacagaag
    15901 gtaaacaagg ccaagaatga gcttatcgcg atgaaggtta tccttcgcga aggccagcaa
    15961 atcgggccta acaagaacat cgaggaatgg caaatcgaag ggtgtcacta ctctggtgat
    16021 aatgtcactg cattggtgac aaaaagtgtc accaaaa
//
LOCUS       DAPHJE010000100        16017 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPHJE010000100 DAPHJE010000000
VERSION     DAPHJE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.101"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(270..695)
                     /gene="arsC"
                     /locus_tag="RH222_003340"
     CDS             complement(270..695)
                     /gene="arsC"
                     /locus_tag="RH222_003340"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV3798310.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTDERLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(708..1997)
                     /gene="arsB"
                     /locus_tag="RH222_003341"
     CDS             complement(708..1997)
                     /gene="arsB"
                     /locus_tag="RH222_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV3798311.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLVALALRLSFTL"
     gene            complement(2051..2404)
                     /gene="arsR"
                     /locus_tag="RH222_003342"
     CDS             complement(2051..2404)
                     /gene="arsR"
                     /locus_tag="RH222_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV3798312.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(2943..3116)
                     /locus_tag="RH222_003343"
     CDS             complement(2943..3116)
                     /locus_tag="RH222_003343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798313.1"
                     /translation="MHPLTHPLPVTAHALLLDNGILTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3144..3227
                     /locus_tag="RH222_003344"
     CDS             3144..3227
                     /locus_tag="RH222_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV3798314.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3281..4633)
                     /gene="gorA"
                     /locus_tag="RH222_003345"
     CDS             complement(3281..4633)
                     /gene="gorA"
                     /locus_tag="RH222_003345"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV3798315.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4705..5547)
                     /gene="rlmJ"
                     /locus_tag="RH222_003346"
     CDS             complement(4705..5547)
                     /gene="rlmJ"
                     /locus_tag="RH222_003346"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV3798316.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5750..7792
                     /gene="prlC"
                     /locus_tag="RH222_003347"
     CDS             5750..7792
                     /gene="prlC"
                     /locus_tag="RH222_003347"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV3798317.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGEKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7800..8552
                     /gene="rsmJ"
                     /locus_tag="RH222_003348"
     CDS             7800..8552
                     /gene="rsmJ"
                     /locus_tag="RH222_003348"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV3798318.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8601..10070)
                     /gene="dtpB"
                     /locus_tag="RH222_003349"
     CDS             complement(8601..10070)
                     /gene="dtpB"
                     /locus_tag="RH222_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV3798319.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10388..10822)
                     /gene="uspA"
                     /locus_tag="RH222_003350"
     CDS             complement(10388..10822)
                     /gene="uspA"
                     /locus_tag="RH222_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV3798320.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11213..11548
                     /gene="uspB"
                     /locus_tag="RH222_003351"
     CDS             11213..11548
                     /gene="uspB"
                     /locus_tag="RH222_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV3798321.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11619..13118)
                     /gene="pitA"
                     /locus_tag="RH222_003352"
     CDS             complement(11619..13118)
                     /gene="pitA"
                     /locus_tag="RH222_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV3798322.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13350..14552
                     /locus_tag="RH222_003353"
     CDS             13350..14552
                     /locus_tag="RH222_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV3798323.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(14867..15919)
                     /locus_tag="RH222_003354"
     CDS             complement(14867..15919)
                     /locus_tag="RH222_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV3798324.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4108 a         4083 c         4165 g         3661 t
ORIGIN      
        1 taatccagta cgtctttaaa ctgctgcact gtatcgctga tcactaagca gatcactgat
       61 acaccatgct ggacgcgata attggaatct cacgcaggga gtaatgtgta aatagcaaaa
      121 tcgcctgatg cgctacgcct gtcaggtcta caaataacat acaatttatt gaattttcat
      181 gactttgtag atcggataag acgcgttagc gtcgcatccg acataaacaa agcgcacttt
      241 accaactatc ggcagtggat atcgccgctt tatttcaacc gcttacccgc ttcatcaacc
      301 actttctcgc catcttcctt ggagaacgcg cctttttgcg catctggcag aatttccagc
      361 accacttccg aggggcggca taggcgagtt cccagcggcg tcaccacaat cggacggtta
      421 atcagaatcg ggtgctgaag cataaagtcg attaaccgct cgtcagtaaa tttatcttcc
      481 gcaaggccca gttcctcata cggctcgacg tttttacgca gcagcgcgcg tacggtaatc
      541 cccatatcgg caatgagttt taccagttca tcgcgcgttg gtggtgtttc cagataatgg
      601 ataatggtcg gttctgtacc gctgttgcgg atcatctcca gcgtattacg cgatgtgccg
      661 caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa
      721 gagagacgaa gcgccagcgc gacgagtgtc acaaacagca caggcagggt catgataatc
      781 cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag tacgtgcagc
      841 cagagcagcg ttgccaggct accaatcggg gtaattttcg gtcccaaatc gcagccaatc
      901 acattggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc atcaatggac
      961 aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa ggcggtgagg
     1021 aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt gagtacgcca
     1081 gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata catgccgagc
     1141 gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt aatcgcatga
     1201 ccgcgtttag cgacgacaaa taatatcagc gcgcccacag ctgcaatggc gctcaccgga
     1261 atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac aacccagcca
     1321 gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag cgccatatcg
     1381 tagttctgcg gaatatcttt gcgaaaatag agatgtaaca tcaccagcgt ggcaacaatc
     1441 gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag gccaaagaaa
     1501 tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc ggtatcggca
     1561 atgaatccgg ccgccatcac gaacgccagc gtagtgcctt tactgaaccc taaagccagc
     1621 agcatggcga tgacaatcgg tgtcaaaata agcgccgcgc catcattggc aaacagggcg
     1681 gcaacggcag caccgagcag gacaatccag gtaaacagca agcgaccacg accattaccc
     1741 cagcgtgaga cgtgcagcgc cgcccattca aaaaagccgg actcatccag cagcaggctg
     1801 atgataatga cggcgataaa cgcagccgtc gcgttccaga cgatattcca caccaccgga
     1861 atatcacccg gatggaccac gcccgtaact aacgccagta ctgcgccgag cgttgcactc
     1921 cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag gacaaagata
     1981 gcgcctgcca gtaacataat gcctcccgga taaaacacat ctgaaaattc atatgtgttt
     2041 agctaaattt ttaactgcaa atgttcttac tgtccccgga acagttttgt cgagccaggt
     2101 tgcggacaat cgcctgaacc ttttcctgtt cacatcgcca ggcctcatca ataattttcg
     2161 ccgcccatgc tggaatatgc ggtgataagc ggtaatgaac ccacttacct tgcttgcggt
     2221 ccagcaatag cccgctttca cgcagcaatg ccaggtggcg ggagatcttg ggctgcgact
     2281 ggtcgagagc agtgcagaga tcgcagacgc ataactctcc cagttcgctg agcagtaaaa
     2341 cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgggt aacagaaatg
     2401 acatattgcg ctcctgattg ttgcaggtag tgtctctctt cgaagcggat aagtcaaaaa
     2461 catatatgac ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga attttgagtt
     2521 ggctattcat ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt tgcgtcggtt
     2581 ttttactttc cagccctgag ttggtggctc tggctggagt gagagagttc ttatctaaca
     2641 gctcaatact attagccagt ggctaacatt gcaagaatta gcgatttctt cagctggcgg
     2701 agggggcact tcggtatata tcgcaatgac gatttatata ctttcactgg gtcatcgtca
     2761 tattaagcct tgtccgaatc cggatgtaaa aaaaccgact ttgcgtcggt tttttacttt
     2821 ccagccctga gttggtggca ctggctggag tgagaatgcc attatctaac gcatcaatgc
     2881 taataccata ctgaaactat tgcaaggaca tgctggtttt ataacctgaa tgtactgtat
     2941 gattatccag ttagctctga ggcattttca ctctggtaat gcgcataaac gctttcaaag
     3001 tcctggtcag aagtacgggt ggtgccgtta actgatgctc tggccggagt gagaatgcca
     3061 ttatctaaca atagggcatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc
     3121 actccatcgc atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa
     3181 ttgcgctgct ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta
     3241 attaagggct aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact
     3301 cttctgccgc cgttgggtga atggcgacgg tattgtcgaa gtcttttttg gttgcaccca
     3361 tcttcagcgc caccgcgaag ccctgcaaca tttcgtccat accaaagcca atgccgtgaa
     3421 taccgacaat cttctcttcc gatccaacgc acaccagctt catgcggcac ggctggcggt
     3481 gagtggtgac ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt
     3541 cgccatactg ctcgcgcgcc tgcggttccg ttaaaccaac agtaccaatc ggcggatggc
     3601 tgaagaccac ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca
     3661 ggcgttcaga gagacggcga cccgctgcaa ctgccaccgg tgtcagctcc actgcacccg
     3721 tgttatcgcc caccgcgtaa ataccttcaa tattggtgtt ttgatattta tcgacgacga
     3781 tatagccttt ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag
     3841 gctcgcgacc aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct
     3901 ccagcgtcag gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca
     3961 gctgcgggcc ttcggcgttc atcacttcga ccagcgtttc ggaaatcatc gggtcgaagc
     4021 tgcgcagcgg cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca
     4081 cgcccgccag ctcaacggcg atgtaacccg cgccaacaac cgccacgcgc tctggcaaag
     4141 caggaagggc gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt
     4201 ggctcggacg accgcctgtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta
     4261 cctccagcgt tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt
     4321 taccgagcac gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga
     4381 tcaacgtttc ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca
     4441 tatggatcgc ttcacggatt tgcgccgcgt gccacatcac ttttttcggc acacagccaa
     4501 catttacgca ggtgccgccc agctctttgg cttcaatcag cgcacatttc tggccgtaca
     4561 tagccgcgcg gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat
     4621 agtgtttagt catgacaaag tgtccttatc gttgattacc gcgattgtag cgcgagacgt
     4681 aataggtgcc agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc
     4741 ggtgcctgcc ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgctgttc
     4801 cagtttccac ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct
     4861 gtctggcagt accgccagtt caatttgcag aattttgcga ataccggtcg cttccagatc
     4921 gtggatcatg cgcttaattt gctgacgcag caccaccgga taccacagtg cgtaagtacc
     4981 agtggcgaaa cgtttgtaac cttctgctat cccgctgacc accgcctgat agtcggtttt
     5041 catttcatac ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc
     5101 cttaagctgc tggaaaccat cggctttttc gacgcgcgca cggctgtctt tctgaaattc
     5161 agaacgcagc aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcttgttc
     5221 gcgcagtagc tggcgagcaa tcaatggcga accggggtag taacgcaact gaccgctacg
     5281 gttgaagtgt tttaccacat tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg
     5341 ccagatacgg gcgatgcctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa
     5401 ctgataacgc ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt
     5461 cagcgactcg atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc
     5521 gtgaaagctg tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc
     5581 cgtattcggg gcgcacaaaa gcttatcagg acagtatacc gaaaagaggc cgccgccgcg
     5641 aaagcgtaac gtttctcatt gaaattcact acacttaacc ccatgctaca cacattatgt
     5701 aaagcgcctg ttgagcgctt ccttaacctc tttaaccagg actgcgcgaa tgacgaatcc
     5761 gttactgact ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc
     5821 agccgtgact aaggcgctga acgactgccg cgaaaatgtg gagcgcgtag tagcgcaagg
     5881 agcaccgtac acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgctggg
     5941 gcgtatcttc tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga
     6001 agcgtacgaa caaaccctgc cgctgctgtc ggaatacagc acctgggtag ggcaacatga
     6061 agggctgtat aaagcgtatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac
     6121 ggctcagaaa aaagcggttg ataacgcact gcgcgacttc gaactctctg gcatcggtct
     6181 gccaatagag aaacaacaac gctacggtga gattgccact cgtctttccg agctgggcaa
     6241 ccagtacagc aacaacgtcc tcgatgcgac gatgggctgg accaaactcg ttaccgacga
     6301 agcggagctg gcggggatgc ctgaaagcgc gctggctgcg gcaaaagccc aggccgaagc
     6361 gaaagagctg gaaggctacc tgctgacgct ggatatccca agctacctgc cggtaatgac
     6421 ctactgcgac aaccaggcct tgcgtgaaga gatgtatcgc gcttacagca cccgcgcttc
     6481 cgatcaaggc ccgaacgccg gtaagtggga taacagcaag gtgatggaag agatcctcgc
     6541 tctgcgtcac gaactggcgc aactgctggg ctttgaaaac tacgccttta aatcccttgc
     6601 tactaaaatg gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg
     6661 cgcgcgtccg caaggcgaaa aagagctggc acaactgcgc gccttcgcca aagcggaatt
     6721 tggcgtcgat gagttgcagc cgtgggatat cgcgtactac agcgaaaaac aaaaacagca
     6781 cctctacagc atcagtgacg aacagctgcg tccgtacttc ccggaaaaca aagcggttaa
     6841 cggcctgttt gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt
     6901 tgatgtctgg catccggatg tacgtttctt cgaactgtat gacgaaaata acgaactgcg
     6961 cggcagcttc tacctcgatc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga
     7021 tgactgcgta ggccagatgc gtaaagctga tggttcgctg caaaaaccgg tcgcgtattt
     7081 gacttgtaac ttcaaccgcc cggtaaatgg taaaccggcg ctgttcactc acgacgaagt
     7141 gatcaccctg ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcatcgaaac
     7201 cgctggtgtt tccggtatca gcggtgtgcc gtgggatgcg gtcgaactgc cgagtcagtt
     7261 tatggaaaac tggtgctggg agccggaggc gctggcgttt atctccggtc actatgaaac
     7321 cggcgaaccg ctgccgaaag agttgctgga taaaatgctg gcggcgaaga actaccaggc
     7381 ggcgctgttt attctgcgtc agctggagtt cggcctgttt gatttccgcc ttcatgctga
     7441 gttccgcccg gatcaggggg aaaaaatcct cgaaactctg gcagaaatca agaaactggt
     7501 tgccgtggtg ccatctccgt cctggggccg tttcccgcac gctttcagcc atattttcgc
     7561 cggtggttat gcggcaggtt actacagcta cctgtgggct gacgtactgg cggcagatgc
     7621 tttctcgcgc tttgaggaag agggcatttt caaccgtgaa accgggcagt cgttcctcga
     7681 caacattctg agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg
     7741 tcgtgaaccg cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag
     7801 tgaaaatctg cttaattgat gaaacaggcg ccggagacgg cgccttatct gttctggcgg
     7861 cccgctgggg gctggagcac gatgaagaca acctgatggc gctggtgtta acgccggaac
     7921 atctggaatt gcgcaagcgt gatgagccaa aacttggcgg catatttgtt gattttgttg
     7981 gcggagcgat ggcgcaccga cgcaaattcg gcggtggtcg cggtgaggcg gtggcgaaag
     8041 cggtgggcat taaaggcgat tatttgccgg atgtggtgga cgccaccgca gggctggggc
     8101 gcgatgcctt tgtgctggct tcagtcggtt gccgcgtgcg gatgctggag cgtaatccag
     8161 tcgttgccgc attgctcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg
     8221 gctggttgca ggagcgtttg cagttaattc acgcctccag cctgacggcg ctaaccgata
     8281 ttaccccacg cccgcaggtg gtctatctcg acccgatgtt cccgcataag cagaaaagcg
     8341 cgctggtgaa aaaagagatg cgtgtctttc agtcgctggt ggggccggat cttgatgccg
     8401 atggattgct ggagcctgca cgcttgctgg cgaccaaacg cgtggtagta aagcgcccgg
     8461 actacgcgcc accgctagcg aatgtcgcca cgccaaacgc ggttgtgact aaagggcatc
     8521 gctttgatat ctatgcaggt acgcccgtgt aaaagtaaag cagggtagcg ataacgctac
     8581 cctgctttct gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac
     8641 ggcaccatca acagcatcac tactgcaacg cccagtgtga ccagaccaat cttaccaaac
     8701 acgttggtat agacgggcaa cgtctcaagc ggatcggtaa tgttgtccgg caccgcggta
     8761 aatgttgcca catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg
     8821 agaataaagc ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag gccaagggcg
     8881 ctaataaaca gttcacctaa gctctggaat aagtacacca gcacgataaa ccatggcgat
     8941 gtcagcccct gggcatccgc aaaccacatt cccgcagctg cagccgtcaa aaagcccaga
     9001 gagcacataa acatgccgag agtaaatttc atcggcatcg agaggtcttt gcctttgtta
     9061 cccagatgcg tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta
     9121 agcgcctgga agctgaccgg gttgatggaa aaaccgagaa tttcatgatg cacgttgttg
     9181 atggcaaaga agttcagcga tgttggcatc tgggcgtaga gaatgtaaaa caccaccgct
     9241 tcgagcatca ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat
     9301 gcctgacgaa agaagatgat ggtgacgacg atggagagaa caatcagcac cagattggcg
     9361 acttctacgt tgtgcatcag ccatgcacat acgaagatca tcaccacgct gccaagtaat
     9421 acgtacaaca gtttgctgaa gctcatcggc ttgaagtcgg gttcagaacc aatgtctttc
     9481 accattccac gacaggcgat gtaaaccagt aatgcgataa ttaaccccgc cccgcacagg
     9541 ttgtaggtga ctgaataacc gaatctatca gcgatcacag gggccagcga taacgctatc
     9601 aacgagccga tattgatcga catatagaac agggtgaatg cgccatcaag ccgcggatct
     9661 ttcggcggat agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg
     9721 acagcgatag tccccagggc gatgaaaatc aggtcaggct taagtagcga catgccggtc
     9781 atgaagtagc caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggtccccagc
     9841 aggtggtcgc cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca
     9901 ccaaaagtga caaaagcctg ctcttgcgag aatccaagct gtttaacgaa gaaaaccgcc
     9961 agtacgccct gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg
    10021 aaaaatgggc gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac
    10081 cttccaatac catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagtttaca
    10141 tctgtcagag attcttaata ttgatgcggt gaatagatta atacactatt acaacagaaa
    10201 ataaccagct gatcacacta atgatggaaa atatgatggt gttaaatgtt gtattggcag
    10261 gagattttga ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta
    10321 acaaggcgcg ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtggg
    10381 gggatgatta ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt
    10441 tgatcagctg acgtgcggaa gacattagtt tgctccagaa gtcctggtgg tgaccacaaa
    10501 ccaccagatc catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc
    10561 tgccgctcag ggtttcagtg attgggtagc ctgcattagt ggaaagctcg gtcagcgcat
    10621 gatgtgtctc ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg
    10681 tgtataggtc agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc
    10741 gagccataga gactgctttc tctaccagaa ctttgctttc cggggagagg tcgaccgcga
    10801 tgagaatgtg tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca
    10861 gctagcgttt cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa
    10921 cccagccgta aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg
    10981 tcaatccgcc ttgcttactc gttagataaa caaattttcg acatgaatat attgatagtg
    11041 gttaaccttc tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat
    11101 gttggtcacc acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg
    11161 ggagcggtag ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag
    11221 caccgtcgca ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt
    11281 ctcatcacta cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata
    11341 tgttgatgga gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt
    11401 ttggtatatc tatgcccaac gttatcgcga tcatcacgat gatgagttta ttcgccgctg
    11461 tgagcgggtg cgtcggcagt ttattctgac tagcgcattg tgtggtctgg tggtggtcag
    11521 cctgattgca ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc
    11581 gggccagtaa actgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagagc
    11641 cagtacagcc cgccggaaag cagtaccgca gccggaaggg taaacaccca ggccatcaga
    11701 atactggtta cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca
    11761 gaagaggaga gtacgtgagt agtggaaacc ggcatcccgg tataactcgc caggccgata
    11821 gacactgccg ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg
    11881 attttctcac cgatagtcgt tgccacacgg cgccagccaa tcatcgtacc gatacctaac
    11941 gccagcgcga ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg
    12001 gacttcagtt tcttcaacag gcgctgatcg tcagcactca cgccaggcat cttcaccact
    12061 ttgtcgatag tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc
    12121 gacagcttgt cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg
    12181 ttaatggtat tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga
    12241 gccggtacta actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag
    12301 taagcttcga cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc
    12361 atgttcacca cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg
    12421 cctttctgac catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc
    12481 gcgatacgcg tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg
    12541 gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc
    12601 agaccgccag caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt
    12661 aatactttcg ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt
    12721 aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag
    12781 taccaggtac ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg
    12841 ccatgagacg atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag
    12901 gcaacactca gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg
    12961 gcgagctgag aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca
    13021 tggaaaccat tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc
    13081 ccggtatgca aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg
    13141 acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt
    13201 tttttgattt gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg
    13261 caaatacttc cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc
    13321 acgcccgttt tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg
    13381 ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc
    13441 tgatcgataa tggtaaaaaa ccagggcgca aaatccttat gtctggcggt gggcgctgca
    13501 actttaccaa cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta
    13561 agtctgcact cgcgcgtttt acccagtggg atttcattga tctggtcaat aaacacggca
    13621 tcgcctggca cgagaaaacg ttaggacaac tcttctgcga tgactccgcg cagcagattg
    13681 tcgacatgct ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag
    13741 tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga actgaacggc atgactgtcg
    13801 gttgcgaaaa gctggtcatc gcgaccggtg ggctgtcaat gccggggctg ggcgcgtcgc
    13861 cgtttggtta taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc
    13921 tggtgccatt cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg
    13981 cggtgccttc cgtgattacc gctgaaaacg gcatcgtttt ccgtgagaac ttactcttca
    14041 cccatcgcgg cttgtctgga ccggcggtgt tgcagatttc cagctactgg caaccgggtg
    14101 aatttgtcag tatcaatctg ctaccggatg tggatctcga aaccttcctg aatgagcagc
    14161 gtaacgcaca tccgaatcag agcctgaaaa acacactggc ggttcatcta ccgaagcggt
    14221 tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg
    14281 tgcgtgacca acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca
    14341 ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactctctt
    14401 cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg
    14461 ttaccggctg gctggggggc tataacttcc agtgggcgtg gtcgagtgcg tgggcttgtg
    14521 cgcaggattt gattgcagca aagtcgtcct gacctatttc ctaaagatga aaattattag
    14581 agtcgcatta aaaatgagct aattttgata gtggttatct tgtgattatt ttctaatgag
    14641 cccgtgaact gaaaccctcc atgcttaata taaggtggat ggaaaggtga ttgaaaactc
    14701 acccagtggc cagatcttta tgaaatatga acaggttgaa aaaaacagta actttgctgt
    14761 tttttatact taaccactat gcattaatgc tgcgttattt catgatgcct aagaaaaacc
    14821 agcgttacgc aaatggtcaa cgctggtttt atccggtacg ttgcaattat tttttagcag
    14881 aacccgcttc taatattgaa acgattgaga acaacgtaaa gcataccgct cccaaaccga
    14941 ccagaactcg cgaaggaata aaatatccca tgtcggtctg cgccatttcc gcaagaaacg
    15001 atgccaggaa aaggcaaaac aggcaggtga agacgggaat catcggtatt ctgttggcta
    15061 acgaacaggt acgtctccat accagtgcca gtagccagac ttttgagaat atgctgtaac
    15121 agatcattcc gaggcaaata agaataatgc cgggagccag tcgggcgctt gcatcggaac
    15181 tgactaagac gtatatcccc tgcagtaccg tgattgagcc gagaaaaata acccaataac
    15241 accacagcca gtgttcttta gttgaaaacg tattacgtgt ttgatggaca attgtggcaa
    15301 caaggccaat tagacaggca cagattgcgg ttaacccaag caatacgtga cccgcgacat
    15361 aatgcggagt tatgtcagca ctacgtaaca gcgtaataga ccaaatgaaa ccgagcaggg
    15421 tgagtaaaac gggaacggca atgaggcaat taccgattaa tgaagaataa gcctgtaccg
    15481 gcgttccatc gctcttgctc cccgctgcat ttttgggaat gagcagaaag tgaccggatg
    15541 acgctgccac cgtcgataca caggcggcaa tcataccaac gccgaaaata acatggccgg
    15601 cgacaaactc gtctgccatc acatcattgc ctgctaacag tgcccaaccc catatcatgg
    15661 tgataattga ccccgcatag ccaataatgg ggaacaatgt attgtaaaac gtattaacgc
    15721 cgcgcgtgag ctgcgaaatg ataataaatg cggtagtgaa taatgccagg catatggccg
    15781 ccagagaaat cagcacatga cccgcgacga aatactcgct ttgccctgaa ctttccagca
    15841 caaagccacc taacgcgatg caaattaatc ccataatcaa agggattaat ttgaaaagcc
    15901 acccgatata tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca
    15961 ttaatgataa tttcagcctg ttaatataac gacgagatat cattaaagac aaatgca
//
LOCUS       DAPHJE010000101        15718 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPHJE010000101 DAPHJE010000000
VERSION     DAPHJE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.102"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            272..676
                     /gene="sdhC"
                     /locus_tag="RH222_003355"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             272..676
                     /gene="sdhC"
                     /locus_tag="RH222_003355"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV3798325.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            670..1017
                     /gene="sdhD"
                     /locus_tag="RH222_003356"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             670..1017
                     /gene="sdhD"
                     /locus_tag="RH222_003356"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV3798326.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFIVVWGV"
     gene            1017..2783
                     /gene="sdhA"
                     /locus_tag="RH222_003357"
     CDS             1017..2783
                     /gene="sdhA"
                     /locus_tag="RH222_003357"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV3798327.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2799..3515
                     /gene="sdhB"
                     /locus_tag="RH222_003358"
     CDS             2799..3515
                     /gene="sdhB"
                     /locus_tag="RH222_003358"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV3798328.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3816..6617
                     /gene="sucA"
                     /locus_tag="RH222_003359"
     CDS             3816..6617
                     /gene="sucA"
                     /locus_tag="RH222_003359"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV3798329.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6632..7849
                     /gene="odhB"
                     /locus_tag="RH222_003360"
     CDS             6632..7849
                     /gene="odhB"
                     /locus_tag="RH222_003360"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV3798330.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            7943..9109
                     /gene="sucC"
                     /locus_tag="RH222_003361"
     CDS             7943..9109
                     /gene="sucC"
                     /locus_tag="RH222_003361"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV3798331.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9109..9978
                     /gene="sucD"
                     /locus_tag="RH222_003362"
     CDS             9109..9978
                     /gene="sucD"
                     /locus_tag="RH222_003362"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV3798332.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10082..10804)
                     /gene="mngR"
                     /locus_tag="RH222_003363"
     CDS             complement(10082..10804)
                     /gene="mngR"
                     /locus_tag="RH222_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDV3798333.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            10973..12889
                     /gene="mngA"
                     /locus_tag="RH222_003364"
     CDS             10973..12889
                     /gene="mngA"
                     /locus_tag="RH222_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDV3798334.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLISKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            12907..15540
                     /gene="mngB"
                     /locus_tag="RH222_003365"
     CDS             12907..15540
                     /gene="mngB"
                     /locus_tag="RH222_003365"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDV3798335.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLSERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         3761 a         3952 c         4338 g         3667 t
ORIGIN      
        1 taaaacccga caaactatat gtaggttaat tgtaatgatt ttgtgaacag cctatactgc
       61 cgccaggtct ccggaacacc ctgcaatccc gagccaccca gcgttgtaac gtgtcgtttt
      121 cgcatctgga agcagtgttt tgcatgacgc gcagttatag aaaggacgct gtctgacccg
      181 caagcagacc ggaggaagga aatcccgacg tctccaggta acagaaagtt aacctctgtg
      241 cccgtagtcc ccagggaata ataagaacag catgtgggcg ttattcatga taagaaatgt
      301 gaaaaaacaa agacctgtta atctggacct acagaccatc cggttcccca tcacggcgat
      361 agcgtccatt ctccatcgcg tttccggtgt gatcaccttt gttgcagtgg gcatcctgct
      421 gtggcttctg ggtaccagcc tctcttcccc tgaaggtttc gagcaagctt ccgcgattat
      481 gggcagcttc ttcgtcaaat ttatcatgtg gggcatcctt accgctctgg cgtatcacgt
      541 cgtcgtaggt attcgccaca tgatgatgga ttttggctat ctggaagaaa cattcgaagc
      601 gggtaaacgc tccgccaaaa tctcctttgt tattactgtc gtgctttcac ttctcgcagg
      661 agtcctcgta tggtaagcaa cgcctccgca ttaggacgca atggcgtaca tgatttcatc
      721 ctcgttcgcg ctaccgctat cgtcctgacg ctctacatca tttatatggt cggttttttc
      781 gctaccagtg gcgagctgac atatgaagtc tggatcggtt tcttcgcctc tgcgttcacc
      841 aaagtgttca ccctgctggc gctgttttct atcttgatcc atgcctggat cggcatgtgg
      901 caggtgttga ccgactacgt taaaccgctg gccttgcgcc tgatgctgca actggtgatt
      961 gtcgttgctc tggtggttta cgtgatttat ggattcattg tggtgtgggg tgtgtgatga
     1021 aattgccagt cagagaattt gatgcagttg tgattggtgc cggtggcgca ggtatgcgcg
     1081 cggcgctgca aatttcccag agcggtcaaa cctgtgcgct gctctctaaa gtcttcccga
     1141 cccgttccca taccgtttct gcgcaaggcg gcattaccgt tgcgctgggt aatacccatg
     1201 aagataactg ggaatggcat atgtacgaca ccgtaaaagg ttcggactat atcggtgacc
     1261 aggacgcgat tgaatatatg tgtaaaaccg ggccggaagc gattctggaa ctcgaacaca
     1321 tgggcctgcc gttctcgcgt cttgatgatg gtcgtatcta tcaacgtccg tttggcggtc
     1381 agtcgaaaaa cttcggcggc gagcaggcgg cacgcactgc ggcagcagct gaccgtaccg
     1441 gtcacgcact gttgcatacc ctttatcagc agaacctgaa aaaccacacc actattttct
     1501 ccgagtggta tgcgctggat ctggtgaaaa accaggatgg cgcggtggtg ggttgtactg
     1561 cactgtgcat cgaaactggt gaagtggttt atttcaaagc ccgcgctacc gtgctggcga
     1621 ctggcggggc agggcgtatt tatcagtcca ccactaacgc ccacattaac actggcgacg
     1681 gcgtcggcat ggctatccgc gcaggcgtac cggtgcagga tatggaaatg tggcagttcc
     1741 acccgaccgg tattgccggt gcgggcgtac tggtcaccga aggttgccgt ggtgaaggcg
     1801 gttatctgct gaacaaacat ggcgaacgct ttatggagcg ttatgcgccg aacgccaaag
     1861 acctggcggg ccgtgatgtg gtggcgcgtt ccatcatgat cgaaatccgt gaaggtcgcg
     1921 gctgtgatgg tccgtggggg ccacacgcga aactgaaact cgaccacctg ggtaaagaag
     1981 ttctcgaatc ccgcctgccg ggtatcctgg aactctcccg taccttcgct cacgttgatc
     2041 cggtgaaaga gccgattccg gttatcccaa cctgtcacta catgatgggc ggtattccga
     2101 ccaaagtgac cggtcaggcg ctgacggtga acgagaaagg cgaagatgtg gttgttccgg
     2161 gactgtttgc cgttggtgaa atcgcttgtg tatcggtaca cggcgctaac cgtctgggcg
     2221 gcaactcgct gctggacctg gtggtctttg gtcgtgcagc gggtctgcat ctgcaagagt
     2281 ctatcgccga acagggcgca ttgcgcgatg ccagcgagtc tgatgttgaa gcatctctgg
     2341 atcgtctgaa ccgctggaac aataatcgta acggtgaaga tccggtggcg attcgtaaag
     2401 cgctgcaaga atgtatgcag cataacttct cggtcttccg tgaaggtgat gcgatggcga
     2461 aagggcttga gcagttgaaa gtgatccgcg agcgtctgaa aaatgcccgt ctggatgaca
     2521 cttccagcga gttcaacacc cagcgcgttg agtgcctgga actggataac ctgatggaaa
     2581 cggcgtatgc aacggctgtt tctgccaact tccgtaccga aagccgtggc gcacatagcc
     2641 gcttcgactt cccggatcgt gatgatgaaa actggctgtg ccactccctg tatctgccag
     2701 agtcggaatc catgacgcgc cgaagcgtca acatggaacc gaaactgcgc ccggcattcc
     2761 cgccgaagat tcgtacttac taatgcggag acaggaaaat gagactcgag ttttcaattt
     2821 atcgctataa cccggatgtt gatgatgctc cgcgtatgca ggattacacc ctggaagcgg
     2881 aagaaggtcg cgacatgatg ctgctggatg cgcttatcca gctgaaagag aaagatccca
     2941 gcctgtcgtt ccgccgctcc tgccgtgaag gtgtgtgcgg ttccgacggt ctgaacatga
     3001 acggcaagaa tggtctggcc tgtattaccc cgatttcggc actcaaccag ccgggcaaga
     3061 agattgtgat tcgcccgctg ccaggtttac cggtgatccg cgatttggtg gtagacatgg
     3121 gacaattcta tgcgcaatat gagaaaatta agccttacct gttgaataat ggacaaaatc
     3181 cgccagctcg cgagcattta cagatgccag agcagcgcga aaaactcgac gggctgtatg
     3241 aatgtattct ctgcgcatgt tgttcaacct cttgtccgtc tttctggtgg aatcccgata
     3301 agtttatcgg cccggcaggc ttgttagcgg catatcgttt cctgatcgat agccgtgata
     3361 ccgagactga cagccgcctc gacggtttga gcgatgcatt cagcgtattc cgctgtcaca
     3421 gcatcatgaa ctgcgtcagt gtatgtccga aggggctgaa cccgacgcgc gccatcggcc
     3481 atattaagtc gatgttgttg caacgtaatg cgtaaaccgt aggcctgata agacgcgcaa
     3541 gcgtcgcatc aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaagtc
     3601 attacccgta ggcctgataa gcgcagcgca tcaggcgtaa caaagaaatg caggaaatct
     3661 ttaaaaactg cccctgacac taagacagtt tttaaaggtt ccttcgcgag ccactacata
     3721 gacaagagct cgcaagtgaa ccccggcacg cacatcactg tgcgtggtag tatccacggc
     3781 gaagtaagca taaaaaagat gcttaaggga tcacgatgca gaacagcgct ttgaaagcct
     3841 ggttggactc ttcttacctc tctggcgcaa accagagctg gatagaacag ctctatgaag
     3901 acttcttaac cgatcctgac tcggttgacg ctaactggcg ttcgacgttc cagcagttac
     3961 ctggtacggg agtcaaaccg gatcaattcc actctcaaac gcgtgaatat ttccgccgcc
     4021 tggcgaaaga cgcttcacgt tactcttcaa cgatctccga ccctgacacc aatgtgaagc
     4081 aggttaaagt cctgcagctc attaacgcat accgcttccg tggtcaccag catgcgaatc
     4141 tcgatccgct gggactgtgg cagcaagata aagtggccga tctggatccg tctttccacg
     4201 atctgaccga agcagacttc caggagacct tcaacgtcgg ttcatttgcc agcggcaaag
     4261 aaaccatgaa actcggcgag ctgctggaag ccctcaagca aacctactgt ggcccgattg
     4321 gtgccgagta tatgcacatt accagcaccg aagaaaaacg ctggatccaa cagcgtatcg
     4381 agtctggtcg cgcgactttc aatagcgaag agaaaaaacg cttcttaagc gaactgaccg
     4441 ccgctgaagg ccttgaacgt tacctcggcg caaaattccc tggcgcaaaa cgcttctcgc
     4501 tggaaggcgg tgacgcgtta atcccgatgc ttaaagagat gatccgacac gctggcaaca
     4561 gcggcacccg cgaagtggtt ctcgggatgg cgcaccgtgg tcgtctgaac gtgctggtga
     4621 acgtgctggg taaaaaaccg caagacttgt tcgacgagtt cgccggtaaa cataaagaac
     4681 acctcggcac gggtgacgtg aaataccaca tgggcttctc gtctgacttc cagaccgatg
     4741 gcggcctggt gcacctggcg ctggcgttta acccgtctca ccttgagatt gtaagcccgg
     4801 tagttatcgg ttctgttcgt gcccgtctgg acagacttga tgagccgagc agcaacaaag
     4861 tgctgccaat caccatccac ggtgacgccg cagtgaccgg gcagggtgtg gttcaggaaa
     4921 ctctgaacat gtcgaaagcg cgtggttatg aagttggcgg tacggtacgt atcgttatca
     4981 acaaccaggt tggtttcacc acctctaatc cgctggatgc tcgttctaca ccgtactgta
     5041 ctgatatcgg taagatggtt caggcaccga ttttccacgt taacgcggat gatccggaag
     5101 ccgttgcctt tgtgacccgt ctggcgctcg atttccgtaa cacctttaaa cgtgatgtct
     5161 tcatcgacct ggtgtgctac cgccgtcacg gccacaacga agccgacgag ccgagcgcaa
     5221 cccagccgct gatgtatcag aaaatcaaaa aacatccgac gccgcgcaaa atctatgctg
     5281 acaagctgga gcaggaaaaa gtggcgacgc tggaagatgc caccgagatg gttaacctgt
     5341 accgcgatgc gctggatgct ggcgattgcg tagtggcaga gtggcgtccg atgaacatgc
     5401 actctttcac ctggtcgccg tatctcaacc acgaatggga cgaagagtac ccgaacaaag
     5461 ttgagatgaa gcgcctgcag gagctggcga aacgcatcag cacggtgccg gaagcagttg
     5521 aaatgcagtc tcgcgttgcc aagatttatg gcgatcgcca ggcgatggct gccggtgaga
     5581 aactgttcga ctggggcggt gcggaaaacc tcgcttacgc cacgctggtt gacgaaggca
     5641 ttccggttcg cctgtcgggt gaagactccg gtcgcggtac cttcttccac cgccacgcgg
     5701 tgatccacaa ccagtctaac ggttccactt acacgccgtt gcaacacatc cataacgggc
     5761 agggcgcgtt ccgcgtctgg gactccgtac tgtctgaaga agcagtgctg gcgtttgaat
     5821 acggttatgc caccgcagaa ccacgcactc tgaccatctg ggaagcgcag ttcggtgact
     5881 tcgccaacgg tgcgcaggtg gttatcgacc agttcatctc ctctggcgag cagaaatggg
     5941 gccggatgtg tggtctggtg atgttgctgc cgcacggtta cgaagggcag gggccggagc
     6001 actcctccgc gcgtctggaa cgttatctgc aactttgtgc tgagcaaaac atgcaggtgt
     6061 gcgtaccgtc taccccggca caggtttacc acatgctgcg tcgtcaggcg ctgcgcggga
     6121 tgcgtcgtcc gctggtcgtg atgtcgccga aatccctgct gcgtcatccg ctggcggttt
     6181 ccagcctcga agaactggcg aacggcacct tcctgccagc catcggtgaa atcgacgagc
     6241 ttgatccgaa gggcgtgaag cgcgtagtga tgtgttctgg taaggtttat tacgacctgc
     6301 tggaacagcg tcgtaagaac aatcaacacg atgtcgccat tgtgcgtatc gagcaactct
     6361 acccgttccc gcataaagcg atgcaggaag tgttgcaaca gtttgctcac gtcaaggatt
     6421 ttgtctggtg ccaggaagag ccgctcaacc agggcgcatg gtactgcagc cagcatcatt
     6481 tccgtgaagt gattccgttt ggggcttctc tgcgttatgc aggccgcccg gcctccgcct
     6541 ctccggcggt agggtatatg tccgttcacc agaaacagca acaagatctg gttaatgacg
     6601 cgctgaacgt cgaataaata aaggatacac aatgagtagc gtagatattc tggtccctga
     6661 cctgcctgaa tccgtagccg atgccaccgt cgcaacctgg cataaaaaac ccggcgacgc
     6721 agtcgtacgt gatgaagtgc tggtagaaat cgaaactgac aaagtggtac tggaagtacc
     6781 ggcatcagca gacggcattc tggatgcggt tctggaagat gaaggtacaa cggtaacgtc
     6841 tcgtcagatc cttggtcgcc tgcgtgaagg caacagcgcc ggtaaagaaa ccagcgccaa
     6901 atctgaagag aaagcgtcca ctccggcgca acgccagcag gcatctctgg aagagcaaaa
     6961 caacgatgcg ttaagcccgg cgatccgtcg cctgctggct gaacacaatc tcgacgccag
     7021 cgccattaaa ggcaccggtg tgggtggtcg tctgactcgt gaagatgtgg aaaaacatct
     7081 ggcgaaagcc ccggcgaaag agtctgctcc ggcagcggct gctccggcgg cgcaaccggc
     7141 tctggctgca cgtagtgaaa aacgtgtccc gatgactcgc ctgcgtaagc gtgtggcaga
     7201 gcgtctgctg gaagcgaaaa actccaccgc catgctgacc acgttcaacg aagtcaacat
     7261 gaagccgatt atggatctgc gtaagcagta cggtgaagcg tttgaaaaac gccacggcat
     7321 ccgtctgggc tttatgtcct tctacgtgaa agcggtggtt gaagccctga aacgttaccc
     7381 ggaagtgaac gcgtctatcg acggcgatga cgtggtttac cacaactatt tcgacgtcag
     7441 catggcggtt tctacgccgc gcggcctggt gacaccggta ctgcgtgatg tcgataccct
     7501 cggcatggca gacatcgaga agaaaatcaa agagctggca gtcaaaggcc gtgacggcaa
     7561 gctgaccgtt gaagatctga ccggtggtaa cttcaccatc accaacggtg gtgtgttcgg
     7621 ttccctgatg tctacgccga tcatcaaccc gccgcagagc gcaattctgg gtatgcacgc
     7681 tatcaaagat cgtccgatgg cggtgaatgg tcaggttgag atcctgccga tgatgtacct
     7741 ggcgctgtcc tacgatcacc gtctgatcga tggtcgcgaa tccgtgggct tcctggtaac
     7801 gatcaaagag ttgctggaag atccgacgcg tctgctgctg gacgtgtagt agtttaagct
     7861 tcacctgcac tgtagaccga cgtggatagg gtgtcggtct acggtttaaa agataacgat
     7921 tactgaagga tggacagaac acatgaactt acatgaatat caggcaaaac aactttttgc
     7981 ccgctatggc ttaccagcac cggtgggtta tgcctgtact actccgcgcg aagcagaaga
     8041 agccgcttca aaaatcggtg ccggcccgtg ggtagtgaaa tgtcaggttc acgctggtgg
     8101 ccgcggtaaa gcgggcggtg tgaaagttgt aaacagcaaa gaagacatcc gtgcttttgc
     8161 agaaaactgg ctcggcaagc gtctggtaac gtatcaaaca gatgccaatg gccaaccggt
     8221 taaccagatt ctggttgaag cagcgaccga tatcgctaaa gagctgtatc tcggtgccgt
     8281 tgtagaccgt agttcccgtc gtgtggtctt tatggcctcc accgaaggcg gcgtggaaat
     8341 cgaaaaagtg gcggaagaaa ctccgcacct gatccacaaa gttgcgcttg atccgctgac
     8401 tggcccgatg ccgtatcagg gacgcgagct ggcgttcaaa ctgggtctgg aaggtaaact
     8461 ggttcagcag ttcaccaaaa tcttcatggg cctggcgacc attttcctgg agcgcgacct
     8521 ggcgttgatc gaaatcaacc cgctggtcat caccaaacag ggcgatctga tttgcctcga
     8581 cggcaaactg ggcgctgacg gcaacgcact gttccgccag cctgatctgc gcgaaatgcg
     8641 tgaccagtcg caggaagatc cgcgtgaagc acaggctgca cagtgggaac tgaactacgt
     8701 tgcgctggac ggtaacatcg gttgtatggt taacggcgca ggtctggcga tgggtacgat
     8761 ggacatcgtt aaactgcacg gcggcgaacc ggctaacttc cttgacgttg gcggcggcgc
     8821 aaccaaagaa cgtgtaaccg aagcgttcaa aatcatcctc tctgacgaca aagtgaaagc
     8881 cgttctggtt aacatcttcg gcggtatcgt tcgttgcgac ctgatcgctg acggtatcat
     8941 cggcgcggta gcagaagtgg gtgttaacgt accggtcgtg gtacgtctgg aaggtaacaa
     9001 cgccgaactc ggcgcgaaga aactggctga cagcggcctg aatattattg cagcaaaagg
     9061 tctgacggat gcagctcagc aggttgttgc cgcagtggag gggaaataat gtccatttta
     9121 attgataaaa acaccaaggt tatctgccag ggctttactg gtagccaggg gactttccac
     9181 tcagaacagg ccattgcata cggcactaaa atggttggcg gcgtaacccc aggtaaaggc
     9241 ggtactaccc acctcggcct gccggtgttc aacaccgtgc gtgaagctgt tgctgctact
     9301 ggcgctaccg cttctgttat ctacgtacca gcaccgttct gcaaagactc cattctggaa
     9361 gctattgatg caggcatcaa actgattatc accatcactg aaggcatccc gacgctggat
     9421 atgctgactg tgaaagtgaa actggacgaa gccggcgttc gtatgatcgg cccgaactgc
     9481 ccgggcgtta tcactccggg tgaatgcaaa atcggtatcc agccgggtca cattcacaaa
     9541 ccgggtaaag tgggtatcgt ttcccgttcc ggtacactga cctatgaagc ggttaaacag
     9601 accacggatt acggtttcgg tcagtcgacc tgtgttggta tcggcggtga cccgatccct
     9661 ggctctaact ttatcgacat tctcgaaatg ttcgaaaaag atccgcagac cgaagcgatc
     9721 gtaatgatcg gtgagatcgg cggtagcgct gaagaagaag cggcagcgta catcaaagag
     9781 catgttacca agccagttgt gggttacatc gctggtgtga ctgcgccgaa aggcaaacgt
     9841 atgggccacg cgggtgccat cattgccggt gggaaaggga ctgcggatga gaaattcgct
     9901 gctctggaag ccgcaggcgt gaaaaccgtt cgcagcctgg cggatatcgg tgaagcactg
     9961 aaaactgttc tgaaataaat atctgtaata agaaatagcc ctcgccgctt ccctctacag
    10021 gaatggcgaa gggctgtcgg tttcgacatg gttggccatc gtatgatggc cttttttgtg
    10081 cttatcgcga tgattttcgt tgcgctatca gggtaaattt atagtcatcg gtattaaaag
    10141 cgttgcggct atattcaaac acccgaccat caactaaata tccacgcgat actttttcaa
    10201 gaatcggctt tgtctggctg atattaagca gacggctcat ctcttcggtt ggcatcagag
    10261 gaatgatttc ctgttcgcta cgatcgataa ccattttctt cacttcttca ataaagtgat
    10321 attttgaatt ttccatgacc tgccaggtga gatccgggaa caacgcaagc ggcatccagg
    10381 tttcttccag cgccattggc ttttgcttgc gatagcgcac gcgcttcaca tgccacacac
    10441 gatcctgcgc ggtgatttgt agctgttgct gaagaaaatc gtcagccgga atcacttcaa
    10501 atatcaggac ttcactgtgt gtatcgacgt gacggtccga cagtttttca tcaaaactgg
    10561 ttaactgaaa aatatcgtaa ttgacccgct cttctttgac gtaagtcccg ctgccctgaa
    10621 tgctttcgag gatctgttgc tcgactaact ggcgcaaagc ctgacgcacc gtaacccggc
    10681 tgacgccaaa ctctgtttgt aatgccgatt cggtaggtaa cgcatcgcca ggtttaagct
    10741 cgccacgcgc aatttgttca cgaatgcgat cggcaatctg ccggtataag ggcttgtgtc
    10801 ccatttttag tatctcatta atacgaattt agccattatg cccgataaat tcatcctgta
    10861 aataatacaa atacaataca aataatttca atcaagtgaa attgatcaca taatggtatt
    10921 gttttatcgg gcacactggc gcgactataa aaacgatcaa gtgaggatca tgatgaatct
    10981 gacgactctg acccaccgcg atgcgttgtg tctgaatgcg cgctttacca gccgtgaaga
    11041 ggccatccac gcgttgactc aacgtcttgc tgctctgggg aaaatttcca gtactgagca
    11101 atttctggaa gaagtgtatc gccgtgaaag ccttggcccg accgccttag gtgaagggtt
    11161 ggctgtgccg catggcaaaa ctgctgcggt aaaagaagcg gcgtttgcgg tcgccacact
    11221 cagcgagccg cttcagtggg aaggcgttga tggcccggaa gcggttgatt tagtggtgct
    11281 gctggcgatt ccccccaatg aagcgggtac tacgcatatg caactgctga cagcgctgac
    11341 cacgcgcctg gcggatgatg agattcgggc gcgtatacag tcggcgacga cgcctgatga
    11401 gttgctctcg gcgctggatg acaagggagg cacgcaacct tctgcctctt tttccaacgc
    11461 gccaactatc gtctgcgtaa cggcctgtcc ggcgggtatt gctcacacct atatggctgc
    11521 ggaatatctg gaaaaagccg gacgcaaact cggcgtaaat gtttacgttg aaaaacaagg
    11581 cgctaacggc attgaagggc gtttaacggc ggatcaactc aatagtgcaa ccgcctgtat
    11641 ttttgcggct gaagtcgcca tcaaggagag tgagcgtttt aatggcattc ccgcgctttc
    11701 agtgcctgtt gccgagccga ttcgccatgc agaagcgttg atccaacaag cgcttaccct
    11761 caagcgtagc gatgagacgc gtaccgtaca gcaagatacg caaccggtga aaagtgtcaa
    11821 aacggagctg aaacaggcac tgttgagcgg aatctctttt gccgtaccgt tgattgtcgc
    11881 ggggggcacg gtgctggcgg tcgcggtatt actgtcgcaa atcttcgggc tacaagatct
    11941 gtttaatgaa gaaaactcct ggctgtggat gtaccgcaag ctgggcggcg ggctgctcgg
    12001 aattttgatg gtaccggtgc tcgcggccta taccgcctat tctctggcag ataaaccggc
    12061 gttagcgcca ggctttgcgg ctggacttgc cgccaacatg atcggctccg ggtttctcgg
    12121 cgcggtcgtt ggcggattga tagccggtta cttgatgcgc tgggtgaaaa atcacttgcg
    12181 tcttatcagt aaattcaatg gattcctgac tttttatctc tacccggtgc tcggtacgtt
    12241 gggagcgggc agtctgatgc tgtttgtggt gggagaacct gtcgcctgga tcaataactc
    12301 gcttaccgcc tggctgaacg gtctgtcagg aagtaacgcg ctgttgctgg gtgccattct
    12361 cggttttatg tgttcctttg accttggagg gccagtgaac aaagccgctt atgcattctg
    12421 cctgggcgca atggcgaacg gcgtttacgg cccgtatgcc attttcgcct ccgtcaaaat
    12481 ggtttcggca tttaccgtaa ccgcttccac gatgctcgca ccgcgcctgt ttaaagagtt
    12541 tgaaattgag accgggaaat ccacctggct gttagggctg gcaggtatta ccgaaggggc
    12601 gatcccgatg gcgattgaag atccgctgcg ggttattggt tcgtttgtgc tgggctctat
    12661 ggtaacgggc gctattgtcg gtgcgatgaa tatcggcctt tcgacacccg gtgccggcat
    12721 tttctcgctc tttttacttc atgataatgg cgcgggcggt gttatggcgg caattggctg
    12781 gtttggtgcg gcattggtgg gggctgcaat ctcgactgca attctcctga tctggcggcg
    12841 tcacgcggtt aagcatggca actatctgac tgatggcgta atgccataaa caaaaaggaa
    12901 acgacgatga aagcggtatc tcgcgttcac atcaccccgc atatgcattg ggatcgggag
    12961 tggtatttca ccaccgaaga gtcacgcatt ctgctggtca ataatatgga agagatcctg
    13021 tgccgactgg aacaggacaa cgaatacaaa tattacgtac tcgacgggca aacggcgatc
    13081 ctcgaagatt atttcgcggt gaaaccggaa aacaaagacc gcgtgaagaa acaggtagaa
    13141 gccggtaagt tgattatcgg cccctggtat acccagaccg ataccacgat tgtttctgcg
    13201 gaatccatcg tccgtaatct gatgtacgga atgcgtgact gcctcgcgtt tggcgagccg
    13261 atgaaaatag gttatttacc ggattccttt ggcatgtccg ggcaactgcc gcatatctac
    13321 aatggatttg gcattacccg cactatgttc tggcgcggat gttcggagcg ccacggtact
    13381 gataaaaccg agtttttatg gcaaagcagt gacggtagcg aagtgacggc gcaggtgctg
    13441 ccgttgggtt atgccatcgg taagtactta cctgccgacg aaaacggatt acgcaaacgc
    13501 ctcgacagtt attttgacgt gctggaaaaa gcgtctgtaa ccaaagagat tttattgccg
    13561 aatggtcatg accagatgcc attgcagcaa aatatcttcg aagtgatgga gaaacttcgc
    13621 gaaatttatc ctcaacgaaa gtttgtgatg agccgctttg aagaggtatt cgagcagatt
    13681 gaagcgcagc gtgaaagtct ggcgaccctg aaaggtgaat ttattgatgg caaatatatg
    13741 cgcgtgcatc gcaccatcgg ttccacgcgc atggatatca aagttgccca cgcacgtatt
    13801 gaaaataaga ttgttaatct gctggaaccg ctggcaacgc tggcctggac gttgggtttt
    13861 gaataccacc acggcttgct ggagaaaatg tggaaagaga tcttaaaaaa tcatgcccac
    13921 gacagtatcg gttgttgttg cagtgacaaa gttcatcgcg aaattgtcgc ccgcttcgaa
    13981 ctggctgaag acatggcgga taatctgctg cgtttctaca tgcgcaaaat tgccgataac
    14041 atgccacaga gtgatgccga caaactcgtg ctgtttaacc tgatgccctg gccgcgggaa
    14101 gaagttatta acaccactgt gcggctgcgc gccagccagt ttaatctacg agacgatcgc
    14161 ggtcagcctg taccgtattt tattcgccat gcccgtgaga tcgatccagg cctaatcgat
    14221 cggcaaatag ttcattacgg taattacgat ccctttatgg agtttgatat acagatcaac
    14281 cagattgtcc cttctatggg ctatcgcacg ctttatatcg aagcgaatca gcctggcaac
    14341 gtgattgcgg caaaaagtga cgctgaaggg atactggaaa atgctttctg gcaaattgcg
    14401 ctcaatgagg atggaacgct acaactggta gataaagata gcggtgtgcg ctatgaccgg
    14461 gtgttgcaaa ttgaagaaag ctctgatgat ggtgatgaat atgactattc acccgcaaaa
    14521 gaagagtggg taatgacttc tgcaacagcg aaaccgcaat gcgagattac tcatgaggcc
    14581 tggcagagca gggctgttat ccgctatgac atggcggtgc cgctcaattt gtcagaacgc
    14641 agcgtccggc aatccacggg cagagtaggg gtggagatgg ttgtcactct gagtcataac
    14701 agcagacgta ttgatgtgga tatcaacctt gataaccagg ctgacgatca tcgccttcgc
    14761 gtcctgatcc ctacaccttt taacaccgac agtgttctgg cagatacgca gtttggttcg
    14821 ctaacgcgcc ccgtgaacga cagtgcaatg aacaactggc agcaagaagg ctggaaagaa
    14881 gcgccggttc cggtatggaa tatgctcaac tatgttgcct tacaggaagg gcgtaacggc
    14941 atggctgtct ttagcgaagg gctacgtgaa tttgaagtca tcggtgaaga gaagaaaacc
    15001 tttgccatta cgttgctgcg tggcgtgggc ttactgggca aagaagatct gcttttaagg
    15061 cctgggcggc cttcgggaat taaaatgcca gtcccggact cacaactacg tggtctgctt
    15121 tcttgtcgcc taagtttatt gagttatacc ggtacgccaa ccgccgctgg tgtagctcag
    15181 caggcgcgag catggctgac tccagtacag tgttacaaca aaatcccatg ggatgcgatg
    15241 aagctcaaca aagccggatt caacgtgccg gaaagttata gtttgttgaa aatgccccca
    15301 gtgggatgcc tgataagcgc acttaagaaa gctgaagacc gacaagaagt gattttacgg
    15361 ctgtttaatc cggctgaatc agcaacctgt gatgcgactg ttgctttcag tcgcgaggtg
    15421 atttcttgct cagaaacgat gatggatgaa cacattacca ccgaggaaaa tcaaggttca
    15481 aatctatcgg ggcctttttt acccggccag tcacggacgt tcagttaccg gcttgcctga
    15541 atagcaatca aaccgaagcc acatatgcgc ggccagattg ttgacaaagg gcgctttgtt
    15601 catgccggat acggcatgaa cgctttattc ggtctacaaa agcaggcaaa ttcaatatat
    15661 tgcagagatg atgtagacac tggcaagcgt agcgcatcag gtaattttgc gtttatct
//
LOCUS       DAPHJE010000102        15572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPHJE010000102 DAPHJE010000000
VERSION     DAPHJE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.103"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..462)
                     /locus_tag="RH222_003366"
     CDS             complement(<1..462)
                     /locus_tag="RH222_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV3798336.1"
                     /translation="MARCALILKISLAMLAFAANSILCRLALQGGHIDPLSFSSLRLA
                     SGAFVLSPFLFYHARTTLSLWRPLSGFYLMAYAVLFSVAYIHLDTGAGALLLFGAVQF
                     AMVIHGLFKGESLSAMRACGLVLALTGIAALLLPGTKAPPLYSALMMIVSGL"
     gene            704..1456
                     /locus_tag="RH222_003367"
     CDS             704..1456
                     /locus_tag="RH222_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3798337.1"
                     /translation="MKKLKFTFKNAAGEELAGLLELPENPKAFALLAHCFTCGKDLKG
                     AARIARKLTEKAIAVLRFDFTGLGNSEGDFSNTNFSSNISDLLCAVDYLRRQYEAPSL
                     LIGHSLGGSAILSIAGEVPEAKAIVTIGSPGELTHVKRLFKDDVENINQHGAYPVELA
                     GRVFTLKKQMLDNIQEHKIAHKVFAMNKPLLIFHATEDDTVLIEQAEKIFKAAKHPKS
                     FISLGKADHLLTHAQDAEYVADIIISWSECYL"
     gene            1483..1796
                     /locus_tag="RH222_003368"
                     /pseudo
     CDS             1483..1796
                     /locus_tag="RH222_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            1850..2062
                     /locus_tag="RH222_003369"
                     /pseudo
     CDS             1850..2062
                     /locus_tag="RH222_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2261..2659
                     /locus_tag="RH222_003370"
     CDS             2261..2659
                     /locus_tag="RH222_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDV3798338.1"
                     /translation="MKNSNEKLVHDFYQALEGYDFLTLKSLCSSDFVFYSQIDNPIHG
                     VDGFIASETATLDGFRGFSMKPEVLISEADKVVAYVVFEGIHNNEMHGYKPTGKKVRI
                     SLMMLLTIEGGKIKEKRAHFDYNDIVRQIS"
     gene            2679..3632
                     /locus_tag="RH222_003371"
     CDS             2679..3632
                     /locus_tag="RH222_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3798339.1"
                     /translation="MKKIAILITDDISAFHLSVPLAIFADIIPDHSLFNVVLCSVQGK
                     TIKTSHGMHITPEYDLSVLRTADMVIVPFWPSPDIVPEPQLTEALREAAQHNATIVGL
                     CLGAYALAYSGLLDARNASTHWLYESDFQSRFPNTHLDMNALYVADDNIITSAGTGAG
                     MDCCLYLVRELYGSNIANQIARRMVIPPHRDGGQAQYIQMPFPSATKDRQIILLVETL
                     RQDLARRWTLDELCEVVSMSRRTFTRRFRQSTGMTLVDWLTTERLREAQVLLESTAHS
                     VQKVSELVGFSSATSLRKKFIAKFGVSPSVWRKTFRGSEAI"
     gene            3703..4479
                     /locus_tag="RH222_003372"
     CDS             3703..4479
                     /locus_tag="RH222_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV3798340.1"
                     /translation="MKFHQIRNATAIIHYAGKKILIDPMLSAKGSFPGFPLTMNRHLR
                     NPLVDLPLPVSEVIDVDAIIVTHTHDDHWDAAARALIPRDMLIFVQNTEDKNELARDG
                     FINLQVLDNEIEYAGIRLIKTHCQHGSDEAFRNPAAAELLGEAMGVILQSDGEKTIYI
                     VGDTIWINDVEQVMHKYQPDIVILNTGYAQVENLGPIIMGKEDVLRTHNIFPKAHIIS
                     THMESVNHFGQTRKEIREYVDLNKITDQVSIPEDGEVVNL"
     gene            4677..5000
                     /locus_tag="RH222_003373"
                     /pseudo
     CDS             4677..5000
                     /locus_tag="RH222_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            5157..6179
                     /locus_tag="RH222_003374"
     CDS             5157..6179
                     /locus_tag="RH222_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDV3798341.1"
                     /translation="MTITTVGIDLAKNVFAVHCVDQNGKTVLVKPKVSRAALPELIAS
                     LPPCVIGMEACSGAHYWARVFRQYGHEPRLMAAKFVSPYRMAGKSGKNDAADAQAICE
                     AVRRPHMRFVPVKDESQQAMQCLHRTRQGFIEEKTATYNRLRGLISEFGVIAPQSTDA
                     LRHIVSEQKNSLPLQVQQCVDDLLEHINRIEDNIAEYDRILSRMAKTDHRSQRLMELK
                     GIGPTTACALVASIGNAHDFKNGRQLAAWLGLTPSQYSSGGKSKLGRITKAGDSYLRT
                     LLVQGARSVLIGAEKRTDSFSRWVCKLVERRGYWRAVVAIAAKNARLCWASLHYGDDF
                     RLYSAS"
     gene            complement(6517..7625)
                     /locus_tag="RH222_003375"
                     /pseudo
     CDS             complement(6517..7625)
                     /locus_tag="RH222_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611760.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7800..7955
                     /locus_tag="RH222_003376"
                     /pseudo
     CDS             7800..7955
                     /locus_tag="RH222_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611384.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8046..12173
                     /locus_tag="RH222_003377"
     CDS             8046..12173
                     /locus_tag="RH222_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3798342.1"
                     /translation="MNIDFSLIRSAPKSRNDSFEALAVQLFRKTCRVPTKSTFISLRG
                     DGGDGGVEAYFRSPDGAVFGVQAKYFFQLASAELTQIDSSLKAALSNHPTLTEYWIYI
                     PFDLTGRVAAGKRGKSQAERFEEWKSKVESEASAKGKSLSIVLCTAAVICNQLLEIDP
                     YGGMRRYWFDDTLLTAAQIQQCLDDAIAFAGPRYTSMLDVVTNAHVGLDFFGGTGDFR
                     EWYETSLTPIVREFHSLNGYGRKSLDILGETCATSATALIEEIIAYCESMRDNDVTAT
                     SVTDLCVALSSLLTLIADARHAQEDKFYKKHGKHSDTESFRQFHAEYMCAFPAGDMDA
                     ARKWEEQAQQLQNLLTSQVIGAATAHSLLLVGPAGIGKTHAIVSAALRRLEHGGFSLV
                     VFGDDFGKAEPWEVLRSKIGLGAAIDRSTLFECIQACAEHTSLPFVIYIDALNESPRE
                     VRWKDKLPELLAQCKSYPDIKICVSTRDTYRNLVVDSRFPGFAFEHIGFSGHQFEAVQ
                     AFAAYYGLDAEITPLFSPELSNPLFLHLACKTLKGEGRDNLDISLPGFTSLFQGHLKH
                     CDVLIREHLHYANPRNLVRAAMMALANTLTHELPQNRTWETCCEALSKIVGTETTPES
                     FLNALAHEGLIILSVVDEDTFLIRLGYQRYGDILRAISLVEALDSDTAKLAEKIAALT
                     EEDAGLLEALAAVLPEKTALEITAEEVGLPSEKAHKLFIQSLVWRSRQSVVEEIDEHI
                     HAALHTPGLWESVYEALFSLSLVPDHRLNATNWLGPFLRQSSLAERDTYLSLAALGSF
                     DNKTAVYSLIHAALFADITHWPAESRRLASLTLAWLTSCADRRIRDLSSKGLSRILAN
                     YPENCQTVISEFAYCDDDYVLERISLAIYSACLLSHQRRNAFMPALPGLLSIASDSKN
                     ILLRDTVQLLVNLLKTGEFPTAVTSQLQHYQTNVSLPSRWPVMADVKPLLDLEHLPSN
                     MVLWGESMAPDFWRYQVESKISGFDLESANISHENIACWLMREALNLGYPGYNHCALN
                     YDRHIGSQYGSGRGRKGYADRLGKKYYWIALHRLLGILASNVPALEDPYSDYEPTSDH
                     LWSVDVRKVDLTDVRDITAESVYPVLMEETNYAFPDRNSDIKGWVRTDDLSPYEACLI
                     RTDKEGEQWVALSHSYWDEDKAPNENSWNSPYLAVRAYYSSALINESIQNFKQKSARD
                     IFQYNQGNSCYRGYLAEYPDSPVYKQLLNNEEDSEAFNFTEVSLLRGSEWEYDYSYTT
                     PERQDSLIAPCQGIIQKLELLWDCQSGWVDRSGKLIAFHHKGVRQSGLFIHRSALNAY
                     LAITGEELIYRRFANRGYFDLAGRNGSQIDLKTWIQYRADKAPVVLREEELPFNC"
     gene            12309..13451
                     /locus_tag="RH222_003378"
     CDS             12309..13451
                     /locus_tag="RH222_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3798343.1"
                     /translation="MNETIEKLIASGKEGPWWDFKQTYHQNNAALVHDILCMANVLHD
                     GDRYLIFGVNDEGVITGVPEDGKQLNQANLIDLLRKVSFAEHHCPDIQLHHITLQHKV
                     LAILQIRNVRMKPYYLTQDYIKEGKTVRAGVVYTRQQDANTPVTSCASPGDVMAMWRE
                     RFNLDLAPADRIVRLLLDYDNWEYDGISEAYYRLDPDYAIHIGYTEKDIGGQHWWSTI
                     SIEQPCHYEYILKYRGLVLERVPIVHYRNEGLQFPFPEMDTLAYPGKRDGFVTDYYAD
                     VFYFVKNTLPYNLLSHIRELHSLPGRNSGITMPLTTQTKPPIIELPFMVFENAGEKEE
                     KLKFIQSQLADFCPDGMPDTASMKTDPELRMEVERQFSWWVFNHMR"
     gene            complement(13735..13926)
                     /locus_tag="RH222_003379"
                     /pseudo
     CDS             complement(13735..13926)
                     /locus_tag="RH222_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006810654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
     gene            13968..14903
                     /locus_tag="RH222_003380"
     CDS             13968..14903
                     /locus_tag="RH222_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798344.1"
                     /translation="MQAIFGFRGNQLVDWDAHVLSHFPLIGELNHPWRWANAGAPELG
                     RWLLDCRRMLLNGNAIDLRQVPAEVTWVPLRAETVVQQRLTAALTRAVTANGQVLVIG
                     NSYDTRGRQQMASQTPGATTVEAVDLVDLTDFGAAFNLDAADALTRLVQFAAGVMTQV
                     GPAALLPRLETLRRGRARNPPTVAEAALVAFAERPGFTLAAEALRLLTRQDNARVYRP
                     EVLRCCLRALQSAAAGGCTFADATVNERERNRHESRPLARRVIGSTLLLKGLEGEVAV
                     ITSPEEMDARHLYVAMTRGSSRLVVCSLTPILTAR"
     gene            complement(14992..15288)
                     /locus_tag="RH222_003381"
     CDS             complement(14992..15288)
                     /locus_tag="RH222_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3798345.1"
                     /translation="MDTIFPVRLLSQLRPLLIGFRKSKGLTQRELSAMLGVTQQTYAR
                     LESNPSKASFERLYKVLNILGVEISLSSAPLSTYTNPANFVEKEFDSPARREEW"
BASE COUNT         4100 a         3681 c         3843 g         3948 t
ORIGIN      
        1 taatccggaa acgatcatca taagtgcact gtaaagcggc ggtgccttcg tcccgggtaa
       61 cagcagggca gcaattcccg tcagagccag aactaaaccg caagcccgca tagcggagag
      121 tgattctcct ttaaataatc cgtggatcac catcgcaaac tgtacggcac caaaaagtaa
      181 cagcgcacca gccccggtat cgagatggat ataggcgaca gaaaacagca ccgcatacgc
      241 catgaggtaa aacccgctca gcggacgcca cagcgataat gtggtcctgg cgtgataaaa
      301 gaggaagggg gacaggacga atgcaccact ggccagcctg aggctgctga atgaaagggg
      361 atcaatgtgg cctccctgca gggccagacg acacagaatg gagtttgccg caaatgccag
      421 cattgccagt gatattttaa gaatgagcgc gcacctggcc attcgagaag tcctcacaaa
      481 agccttgtaa actaccatct agttggtagc atagcgtaag tgatcgccga acaggaagca
      541 ataaccagct tccaaactgg aaggtgagga caggcattcg gaatgaattc tgtttcacat
      601 ccccagtgga ggacggaaaa cgcgttatgc cagatgcaga gcagaagatt atttttctcc
      661 tgcgatcaaa catatgacat cagtttaagg cccggaatat cctatgaaaa aactaaaatt
      721 tacgttcaaa aatgcggcgg gtgaagaact ggccggattg cttgaacttc ctgaaaatcc
      781 taaagcgttt gcgctgttag ctcattgttt tacgtgtggc aaagacctca aaggcgcggc
      841 gcgtattgcg cggaaactga cagagaaagc tatcgccgtt ctccggtttg attttactgg
      901 tctgggaaac agcgaagggg atttttccaa caccaacttc tcatccaata tttcggacct
      961 gctttgcgcg gtagattatc tccgccgaca gtacgaagcg ccttctttgc tcatcggtca
     1021 tagcctgggg ggatcggcca tcctgtctat cgccggcgaa gttcctgagg caaaggccat
     1081 tgtcaccata ggatcacccg gagaactgac acacgtaaaa cgtcttttta aggatgatgt
     1141 tgaaaacatt aaccagcatg gagcctatcc cgttgagctg gccgggcggg tatttacgtt
     1201 aaagaaacag atgctggaca atattcagga acacaaaatc gctcataagg tgttcgccat
     1261 gaacaaacct ctgctgattt ttcatgcgac tgaagatgat accgtgttaa ttgagcaggc
     1321 cgagaaaata ttcaaagcag ctaagcatcc caagagtttc atttccctgg gtaaggctga
     1381 tcaccttctg acacatgctc aggatgctga atacgttgcc gatatcatta tatcgtggag
     1441 cgaatgttac ctataaacag agtaaatgcg ttcaaactcg tagcgctgag ggcgattttc
     1501 tgcagagatc ggacgctgtt tatgctggac tgattttgcg ataaaggctt aggccgatgc
     1561 atacaggcgg ggctgaataa gggtgaggcc cttgggcgag catccttcat gcaccggctg
     1621 aatgaaatca gggatctggg ggggggagga tcagcgttac cacgccaccg gcgctgacac
     1681 tacatacggc ggcgataaca ctctggaata cggtttatat agaaagaacc attgagccgc
     1741 ttaagcaaaa aggagtccct gttaatgaac tgctggtctc tcatccttct ccactgctgt
     1801 acaaaatata gtcttagcgt gggataattt ccgttttcca agcggacccc aatttgaaag
     1861 ggatttgctt cttgagcgta cgcactctgg tattgcgaga gcgaaagctg ccggaaagcg
     1921 gtttggccgt ccttcaacac tgaatgaaga acagaaactt tctgtgattg cacgtattaa
     1981 ggccggggcc agtatcagcg ccattgctcg ggagtttaat acaacccggc aaaccatact
     2041 cagggtaaag gcaggacagt aatgaccgtg agttctcagc tggacgttat gctatgtgct
     2101 atgagcgggc ttaacacagg cagatgttga aacccagcgg ggggagcctt tttctcataa
     2161 caatggccca atcatgttga ttaatgtctt tttggtcact cacactcact tgtgtacacc
     2221 acgtagaatg tccttactcc agacaaaaag gtgaatgacc atgaaaaact caaatgaaaa
     2281 acttgtacat gatttttatc aggctcttga aggttatgat tttttaaccc ttaaatcact
     2341 ttgctccagt gactttgttt tctattcaca gatagataac cccattcatg gagttgacgg
     2401 ttttatcgca tctgaaactg ccacacttga tggattccgg ggcttcagca tgaagcctga
     2461 agttcttatc agtgaagcgg ataaagtcgt agcttatgtt gtttttgaag gtatccacaa
     2521 taatgaaatg cacggatata aaccaaccgg gaaaaaagta cgtatttctc tgatgatgct
     2581 gctgactatt gaaggcggta aaattaagga aaaacgtgcg cactttgatt ataacgatat
     2641 cgtacgtcag attagttaat cttacaggag agtatgaaat gaaaaagata gccattttga
     2701 taaccgatga tatcagtgca tttcatctct ccgtaccact ggcaattttt gcagatatta
     2761 tcccggacca ttcattattt aacgttgttt tatgctccgt gcagggaaag actataaaaa
     2821 cctcacatgg tatgcatatt acaccggaat atgacttatc ggtgttacgt actgctgaca
     2881 tggttatagt tcctttctgg ccatcgcctg acattgtacc ggagccacag ttaacggaag
     2941 cactccggga agctgcacag cataatgcca ccattgtggg tttgtgcctt ggtgcctatg
     3001 ctctggccta ttcaggacta ctggatgcaa gaaacgcgtc gacacactgg ttgtatgaga
     3061 gtgattttca gtcacggttt ccgaataccc acctggatat gaatgcgctc tatgtagctg
     3121 acgataatat tattacttct gccggtacag gtgcgggtat ggactgctgt ctgtatctgg
     3181 tcagggagtt gtacgggagc aatattgcca atcagatagc aaggcgaatg gtgatcccgc
     3241 ctcatcggga cgggggacag gctcagtata ttcagatgcc ttttccatct gcaacaaagg
     3301 atcgccagat aattctgctg gttgaaacgc tgcgccagga cctggccaga cgctggacgc
     3361 tggatgaact ttgtgaagtc gttagcatga gccgacgaac ctttacacgc cgcttccgac
     3421 agtcgacggg catgacactg gttgactggt taacaacgga gcgtctgaga gaagcgcagg
     3481 tattactgga atctacagcc cactctgtac agaaggtatc tgagctggtt ggttttagtt
     3541 ccgcaacatc tttacggaaa aagtttatcg caaaatttgg cgtatcgccg tccgtatggc
     3601 gaaaaacatt caggggttca gaagcgatat aaaacttctg ccagctggaa agtaaattaa
     3661 tcctcttact gtgttctgtt aatacagaac tggagttcta ttatgaaatt tcatcagatc
     3721 cgtaacgcca ctgcaattat tcattatgca ggaaaaaaaa tcctgattga cccgatgtta
     3781 tcagcaaaag gttcattccc tggttttcca ttgacgatga accgccattt acggaaccct
     3841 cttgttgact tacctcttcc ggtatcagag gttattgatg tagatgctat tattgttact
     3901 catacgcatg atgaccactg ggatgctgct gcccgggcgc tgatccctcg cgatatgctt
     3961 atttttgtac agaatactga agacaaaaac gaactggccc gcgatggatt tattaaccta
     4021 caagttcttg ataatgaaat tgaatacgcc ggaattcgct taattaaaac tcactgtcaa
     4081 catggtagtg atgaggcatt ccgcaacccc gctgcggcag agctattagg tgaagctatg
     4141 ggggttattc tccagtctga tggcgaaaag actatctata tagttggtga tactatctgg
     4201 attaatgatg tagagcaggt tatgcataaa tatcagcctg atatcgttat cctgaatacg
     4261 ggatatgcgc aggtggaaaa tctggggcct attatcatgg gtaaagaaga tgtgctaagg
     4321 acacataaca tatttccaaa agcacatatt atttccacac atatggaatc tgtaaatcat
     4381 tttggacaaa cccgaaaaga aatccgtgag tatgtagatc ttaataagat tactgaccag
     4441 gttagtatcc ctgaagatgg tgaagtcgtt aatctttaat tatacatccc cacaacttta
     4501 atcaggcagt tactcattat ggttttactg gtgactgccg gccctcttag cattcgaaat
     4561 tttttatgtc acccgattta gttccgggca ctttttgaga tttccggggt ttcagccatc
     4621 agccggtact cttccggtgt caggttattc agggattcgt gggggggggc gctgttatag
     4681 cgcgagcagt ctttatgcac cggctggggg aaatcagaga ccggggactg gaaaaccaga
     4741 gctaccgggc cagcgggctg acattgctga ctgcggcgat ctcactgtgg aatacggtat
     4801 atatagaaag agccatagat tctctgaagc gaaaagggat cccactaaat gaacagctga
     4861 tttctcatct gtccccgctg ggatgggaac acatcaactt gagtggagat tacgtctggc
     4921 gaacaaacct caagctggga cagggaaaat accgttcgtt acgctcagta gatagcagtc
     4981 tgtataaaaa acaggcttaa cgtaggatat tttccgtttt ccaagcgggc ccctaattta
     5041 tcagtgtatt acaggttaaa atttgatctt aacgaatgga agggacttcc cctggatatg
     5101 tgcctgatta gctcttgtgc aatcatgggg taaacatagc cctggaggga ttcgttatga
     5161 ctatcactac tgtcggtatc gatcttgcta aaaatgtgtt cgctgttcac tgcgttgatc
     5221 aaaatggaaa aactgttctg gttaaaccta aagtatcgcg tgctgcactt cctgagttaa
     5281 ttgcaagcct gcctccctgt gttatcggga tggaggcgtg ctcaggcgcc cattactggg
     5341 caagagtgtt tcggcagtat ggccatgagc cccgtttgat ggctgcaaag ttcgtatcgc
     5401 catatcgcat ggccggtaaa tcaggaaaaa atgatgcagc tgatgcccag gctatttgtg
     5461 aggctgtccg tcgcccgcat atgcggttcg tgccagtgaa ggatgagagc cagcaggcga
     5521 tgcaatgtct acaccgtacc agacaaggtt ttatcgaaga gaaaacagca acatataacc
     5581 gattaagagg tttgatatct gaattcggcg tcatcgctcc acaaagtaca gatgccctgc
     5641 gccacatcgt ttctgaacag aagaattctt tgccgcttca ggttcagcaa tgcgttgatg
     5701 atctgctgga acacattaat cgcattgaag ataacatcgc tgaatatgac cggatcctgt
     5761 cccgcatggc aaaaacggat caccgcagcc aacgactgat ggaactgaaa gggattggtc
     5821 ccacaacggc ctgtgcgctg gtcgccagta tcggtaatgc ccatgatttt aagaatggac
     5881 gccaactggc agcctggctg ggacttacac catcacagta cagcagtggc ggaaaatcaa
     5941 agcttggcag aataacgaaa gcgggcgatt catatctgcg aacgcttctg gttcaggggg
     6001 cccgttcggt tttgattggt gcagagaaaa ggactgactc attcagccgt tgggtttgta
     6061 agttggttga gcgcaggggg tactggcggg ctgttgtagc catcgccgcc aaaaacgcga
     6121 ggctgtgctg ggcatcgttg cattacggtg atgatttcag gctgtactca gccagctaaa
     6181 gcacaaagca gtataaccat ctgacctgca actcgttgat gaaaaagggt tagaccccgt
     6241 gaggcctatc tggctattgt acaggatata cgtatccgct taacgaacaa gaacctcacg
     6301 cgcgtctttc atcagggccc gaatcgataa cgatttatca tggccgttta tagtaccgca
     6361 gtctctcccc tttattttac tgacgagcag acagaaacta taaaaccggc gttgacatgc
     6421 cggggaagcc cttatagcgt tgacatgccg gggaagccct tatagtacaa tattttccgt
     6481 ttcccaaacg gagcctataa tcaggaataa ccggcttcag gttggttgct tattctggca
     6541 accggcagat ttctcggcga attcgcacgg agtcatccag cccagtgcag aatggggacg
     6601 actctggtta tagtgtatgc gccagacctc gattttgcac cgggcatcct ccttggacat
     6661 aaaccagttc tcgttcaggc actcctgccg cagcctgccg ttgaatgact ccaccgtagc
     6721 attatccgtg ggcgtgcctg gccgtgagaa atcgatccgg atatcacgtt cgtacaccca
     6781 tttatccagc attttccctg caaattcaga gccattatct gtcttcagta gctgcggtaa
     6841 tgggcgtctc aacgcgatag tgtccagcat ctccgccact tctgaagagc gcaggttctg
     6901 gcccacgcag atccccagac attcgcgcgt gtagagatcg attaccgtca gcaggcgaag
     6961 ccggcgaccg tcaaacagcg catcggagac aaaatccatc ccccatacat gattgggata
     7021 cagcccctgc ggctgtggct gacgtttcag cgcagattta ttgcggcggg ggcgtttcag
     7081 ccgcagcgat aatccctgct cgcggtaaag ccggtaaaca cgcttgtgat tatcgcgcca
     7141 gccttcccgt ttcagcatga cgtggactct gcggtagcca tagtggacac gggtttcggt
     7201 tatctccctg atgcgcagtc gcagtgcgct gtcgtctgcc gcaacggagc ggtaccggaa
     7261 cgaactgcgg ctgatcttca gcgcaaagca gacctgtctt tcactggcac cgtagcgggt
     7321 ctgtaagtcc ctgacccact cgcgcaggcg tgccagcgtc agctcttttt tgccagcacg
     7381 tcctgcagca tggccttgtc gagactcagg tcggcaacca gcttcttcag gcgaaggttt
     7441 tcctcttcga gctgccgcat atgtttcagc tctgaaggag aaatcccgcc gtactttttg
     7501 cgccaggtgt agaacgtggc atcggagatg cccagcttgc ggcagacttc cggtacgggc
     7561 gtacccagct cagcctgctt cagggcaaag acaatctgct cttcggtaaa tcgtgacttt
     7621 ttcatcggca caacctccgc tcaggggagt gaatatcatg ccggaattct gtttctgaat
     7681 ggagcaggat ttcgggtcag ggtcagaccc caccccggta gacgatcctg ccctatagtt
     7741 tggagtgacc ccgagcccgt agacaaatct gccctatagt ttgagcatag gacgagtcta
     7801 tgggcacacc acgttttacc cctgaattta aggaagaggc tgtccgccag ataaccgagc
     7861 gcggttattc tgttgcctaa gtatcagacc gtctgggcgt ttctgcgcac agcctctaca
     7921 agtggctacg ggctatcaag cctgataaca gcgaacatct tacctcgcct caataccaat
     7981 acatttttaa tgggtataat atgtgactgc ttttgccgca ttattgacag gaacaaggac
     8041 aggtgatgaa tattgatttc agtttaattc gcagcgcccc caaaagccgt aacgatagct
     8101 ttgaagcact cgccgtacag ttatttagga aaacctgtcg agtaccgaca aaatcaacat
     8161 ttattagtct gcgtggagat ggtggagacg gtggcgttga ggcatatttc cgctcaccgg
     8221 acggtgccgt attcggtgtt caggcaaaat actttttcca gcttgcttcc gcagagctta
     8281 cacagattga tagttccctt aaagccgcgc taagcaacca tcccacacta accgaatact
     8341 ggatttatat accgtttgac ctgaccgggc gtgttgctgc gggaaagcga ggaaaaagcc
     8401 aggcggaacg ctttgaagaa tggaaaagta aagtcgaatc ggaagcgtca gcgaaaggga
     8461 agtcactttc tattgtcctt tgtaccgcgg ctgttatctg caatcaatta cttgagatag
     8521 acccttacgg aggaatgcgc aggtattggt ttgatgacac gttgctgaca gcagctcaaa
     8581 ttcaacaatg tctggatgac gccattgctt ttgcagggcc aagatatact tcaatgctgg
     8641 atgttgtgac gaatgctcat gttggtctgg atttttttgg tgggaccggt gactttcgcg
     8701 agtggtatga aacatcatta acaccaatcg ttcgagagtt ccattcactg aatggatacg
     8761 gacgcaaatc gctggatata ctcggcgaaa cctgtgctac atctgcgacg gcattgattg
     8821 aagaaataat cgcctactgt gagagcatga gagataacga tgtcacggcc acatcggtta
     8881 cagatctttg cgttgctctg tcatccctat tgacacttat cgctgatgcc cgccatgctc
     8941 aagaagataa attttataaa aagcatggca agcatagtga tacagaatcg ttccgacagt
     9001 tccacgcaga gtatatgtgt gcatttcctg ccggagatat ggatgcggcg agaaagtggg
     9061 aagagcaggc gcagcaactg caaaatttgt tgacttctca ggtcattggt gctgcaacag
     9121 cacattcctt actgctggtt ggcccagcgg gtatcggcaa aacccacgca attgtcagcg
     9181 ctgcattgcg tcgactggaa catggtggtt tttccttggt cgtctttgga gacgactttg
     9241 gcaaagcaga gccttgggaa gtgctgcgca gtaaaatagg gctgggtgcc gccatcgatc
     9301 gttcgacatt atttgaatgc atacaggcct gcgccgaaca tactagctta ccttttgtca
     9361 tttatatcga tgccttgaac gaaagcccgc gagaagtgcg ctggaaggat aagcttcccg
     9421 aattactcgc tcaatgcaag tcttatccag acatcaaaat ctgcgtttca acccgagata
     9481 cctatcgcaa tcttgtggtc gattcacgct ttccagggtt tgctttcgaa catatcggtt
     9541 tttcaggaca tcaattcgaa gcggtacaag ctttcgcagc ctactatggg ctggatgcag
     9601 agattacacc acttttttca cccgaactca gtaatccttt atttttacac ttggcctgta
     9661 aaacgctaaa gggcgaaggc cgtgacaatc tggatatttc tttgccgggt tttacctctc
     9721 tgtttcaagg acatctcaaa cattgcgatg ttttaattcg agaacacctc cactacgcaa
     9781 accctcgcaa tctggtaagg gctgcaatga tggcactcgc gaataccctg acacatgagt
     9841 tgccgcagaa ccgaacctgg gaaacctgtt gcgaagcact gagcaaaata gtgggaactg
     9901 agaccacacc tgaatccttt ttaaatgcat tggcacatga aggcctcatt attctttctg
     9961 ttgtagatga ggataccttc ctgatccgtc tgggttatca acgctacggt gacatactcc
    10021 gtgctatcag ccttgtggaa gctcttgatt cggatacagc aaaactcgcg gagaaaattg
    10081 cagcgttaac agaagaagat gctggattgc tggaagctct tgccgccgtg ctgccagaga
    10141 aaactgctct tgaaattact gctgaagaag taggattacc atccgaaaaa gcccataagc
    10201 tgttcatcca gtcattggtt tggcgctccc gacaaagtgt agtggaagaa attgatgaac
    10261 acatccatgc agcactgcat acacctggat tatgggagtc ggtttatgaa gcgctgtttt
    10321 cacttagtct ggttcctgac catcgtctaa acgcaactaa ctggctgggg ccatttttac
    10381 ggcagtcatc cttagctgaa cgtgacacct acttgtcatt agctgcgctg ggatcatttg
    10441 ataacaagac tgctgtctat tcactcatcc atgcagcact atttgctgac ataacccatt
    10501 ggcctgctga aagccggagg ctggccagtc taacacttgc ctggctcact tcgtgtgctg
    10561 accgccgaat cagggattta tcctcaaaag ggctaagcag aatcctggca aactacccgg
    10621 agaactgcca aacagtaatc agtgaatttg catattgtga tgatgattac gtattagagc
    10681 gtattagcct tgctatctac agtgcatgct tattgtcaca ccaacgcaga aatgcgttta
    10741 tgccagcgct ccctggtcta ttaagcattg cgtcagatag caagaatatt ctgctccggg
    10801 atacggttca gctattagta aacttgttga aaacaggaga atttcccaca gccgtaacaa
    10861 gccaattaca gcattaccag acaaacgtat cattaccatc acgatggcct gtaatggcgg
    10921 atgtcaaacc cctcctagat ctggaacatt taccatcaaa catggtgctc tggggagagt
    10981 ccatggcccc ggatttctgg cgttatcagg tggaatcgaa gatttccggc tttgacttgg
    11041 agagcgccaa tatcagccat gaaaacattg cctgttggtt aatgcgagaa gcacttaatt
    11101 taggatatcc cggttataac cactgcgcgc tcaattatga tcgccatatc gggagtcagt
    11161 atggctcggg acggggtaga aaagggtatg ctgaccgact cggtaaaaaa tattactgga
    11221 tcgccttaca tcgactactg ggcattctgg ccagtaatgt tcccgcactg gaagacccat
    11281 attccgacta cgaacctaca agtgatcatc tatggtcagt cgacgtccgt aaagtcgacc
    11341 tgaccgatgt acgcgacatc accgcagaaa gtgtctatcc agtactgatg gaagaaacaa
    11401 attatgcatt ccctgaccgc aattcagata tcaaaggttg ggttcggacc gatgatttgt
    11461 caccttatga agcttgtctg attcgaactg acaaggaagg ggaacagtgg gtagcgcttt
    11521 cacacagcta ttgggacgag gataaagcgc cgaatgaaaa tagctggaat tcaccttacc
    11581 tggcagttcg tgcttattat tcaagcgcac tcataaatga aagcatccag aactttaaac
    11641 agaaaagtgc gcgcgatatt ttccaatata atcagggaaa tagctgttat cgcggttatc
    11701 ttgctgaata tcctgacagc ccagtgtaca aacaacttct taataatgag gaagatagtg
    11761 aagcatttaa ttttacagaa gtcagtttac tacgcggaag cgaatgggaa tatgactact
    11821 catataccac gcccgagcgc caggatagcc tcattgcgcc atgccaggga atcattcaaa
    11881 aactcgaact tttatgggat tgtcaaagcg gttgggttga tcgttctggc aaacttatcg
    11941 ccttccatca taaaggtgta agacaaagcg gacttttcat tcatcgttcg gcgttgaacg
    12001 cctacctggc cataacaggt gaagaactta tatatcgccg ttttgctaac aggggatatt
    12061 ttgatttagc tggccgtaac ggctcgcaaa tagacctgaa aacttggatc cagtaccggg
    12121 cagacaaggc gccagtagtt ttacgagaag aggaactgcc gtttaactgc tgacaacgat
    12181 acttattaag taatcaactg gctcttctgg catcgaatgc cagaagagcc atttcacact
    12241 accaatttaa gtagactgaa ggaatacttg gtacaagcaa acgcacgcca tatcggatag
    12301 aggggactat gaacgaaacc atcgaaaaac tgattgccag tggaaaggaa gggccatggt
    12361 gggactttaa acaaacctac caccaaaaca atgcagccct tgttcacgat atcctctgca
    12421 tggcaaacgt cctgcatgac ggtgaccggt atctgatttt tggcgtgaat gatgaagggg
    12481 tgattaccgg cgtgccggaa gatggtaagc aacttaacca ggcaaattta atcgatctgt
    12541 taagaaaagt cagttttgca gagcaccatt gtccggatat tcagctgcac cacatcacac
    12601 tccagcataa ggtgctggct attctacaaa ttcgtaacgt cagaatgaaa ccctactatc
    12661 tgacccagga ctatataaaa gagggaaaaa ctgtcagagc cggtgtcgtt tacacccggc
    12721 aacaagatgc caatacgccc gtaactagct gtgccagccc tggagatgtc atggcaatgt
    12781 ggagagagcg ctttaacctg gacctggcac ccgctgaccg catagtcaga ctgcttctgg
    12841 attacgacaa ttgggaatat gacggtatca gcgaggccta ttatcggttg gacccggatt
    12901 atgccattca tattggctac acagaaaaag atataggagg acaacactgg tggtcaacga
    12961 ttagcattga gcaaccttgt cactatgaat acatcctcaa ataccgtggg cttgtacttg
    13021 aacgagtgcc aatcgtacat tacagaaatg agggtttaca gtttcccttt ccagagatgg
    13081 atacgctggc ttatccagga aaaagggatg gattcgtaac tgattattat gcagacgttt
    13141 tttacttcgt aaaaaacacc ttaccctaca atctgttgag tcatatcaga gaattacact
    13201 cactgcctgg ccgtaattca ggcataacca tgccgttaac cacccagacc aaaccgccga
    13261 ttatcgagct gccattcatg gtgtttgaga acgcagggga aaaagaagaa aagctaaagt
    13321 ttattcaaag tcagcttgct gatttttgtc cggatggtat gcctgataca gcatcgatga
    13381 aaactgaccc agaactaaga atggaagtag agaggcaatt ttcctggtgg gtttttaacc
    13441 atatgcgttg agccgaagcc agtaaccctg cctgggagtt gttcattttt gacgttgcaa
    13501 ctaaaggtca gcgctacgat agcgtgtttt agtgcgcact cgcgtatttt ctccctcttg
    13561 ggtgaatttg gcctactttc gcgggccata tactgcccca cgcacgacga tgtgctttaa
    13621 gagagtcaac ggcaatacca ataaaaagcg ctcggttgca cgatgtggac agcatcctga
    13681 tatggctccc gtggacttag cccccggccg cgtccaacat agcgaagcca gcactcaggc
    13741 tccatgacat tttttatact ttactcccga cccgcaggga caaggttcat ttctgcctac
    13801 cgccccgaac aatcgcgtta cttcggattt agccgccttg taagcaagct gcgcgatgta
    13861 gttcgactga ttttcaacct ctagtcgctg ctcacctgtt aacccttcaa aaaagggggc
    13921 taaccgcgct atcaacggtg ctgcctgcct gtgtactggg tgaccccatg caggccattt
    13981 ttggctttcg ggggaaccag ctcgtggact gggatgcaca tgtactctct cactttccac
    14041 tgattggtga gctcaatcat ccatggcgct gggctaacgc aggggcgccg gagctcggtc
    14101 gctggcttct ggactgccga cggatgttgc tgaatggtaa cgccattgac cttcgccagg
    14161 tgcctgctga ggtgacctgg gtgccgttac gggcagaaac agttgttcag cagcgcctta
    14221 ctgcagccct gacacgcgct gtcaccgcaa acgggcaggt actggtgatt ggaaacagct
    14281 atgacacccg cgggcgtcag cagatggcca gccagacccc gggagcaacg accgtcgaag
    14341 ccgtcgattt agtcgacctt accgactttg gtgcagcgtt taacctagac gctgcggatg
    14401 cgttgacgcg cctggtgcag tttgccgcag gcgtcatgac ccaagttggc cccgcagcat
    14461 tgctgccccg tctggaaacg ctacgaagag ggcgagcccg caacccgccg acggttgccg
    14521 aagcagcgtt agtggcattt gcagagagac ccggttttac gctggccgct gaggcactgc
    14581 gccttctgac ccgccaggat aatgcgcggg tctatcggcc agaggttttg cgctgttgtt
    14641 tgcgcgcctt gcagtcggct gctgccggag gctgcacatt tgcagacgcc acggtaaacg
    14701 agcgcgaacg caatcgccac gagagtcgac cattggcccg gcgtgtaatc ggcagcacat
    14761 tgttgctaaa gggactggaa ggggaggtgg cagtcatcac atccccggaa gagatggatg
    14821 cacgacacct ctatgtcgct atgacgcggg gatcttcgcg gctggttgtg tgctcgctaa
    14881 ctcccatatt gacggcccgc taactccgat gcaaagcgcc cttgctcgca aatcgcacct
    14941 tccttaaggg cagtgcctga cgtacagccc tatcaaatag cctggcttct atcaccattc
    15001 ctcacgcctt gccggtgaat caaactcctt ttctacaaaa tttgcagggt ttgtataagt
    15061 tgaaagcggt gcagaactga gcgaaatctc cacccctaaa atatttaaca ctttgtatag
    15121 acgctcaaaa cttgcttttg aaggatttga ctcaaggcga gcataagtct gctgggttac
    15181 acccaacatg gcagataatt ctctttgagt taatcctttt gatttacgaa aaccaattag
    15241 aagtggacgt aactggctaa gaagtctaac gggaaaaata gtatccataa gtaggtggcc
    15301 atgtcgatta cttcataata aattcactcc tcgtaagaaa cagcctgtaa ggtgtcttat
    15361 tactttacag cttacaaggt gtaagggtga actcgttgta ttaactaata ctgctcaaat
    15421 tttaaaggtt gcatctcgcg ctggctggga ttatatttat taaggactag catatagtgg
    15481 tgtacgctgt tatcctcttt gtaatgaggc ttatcagtgt gtgaagggag agggcggact
    15541 ctgctttgcc ctctctcttt catggactta tt
//
LOCUS       DAPHJE010000103        15568 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPHJE010000103 DAPHJE010000000
VERSION     DAPHJE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.104"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..663
                     /locus_tag="RH222_003382"
     CDS             97..663
                     /locus_tag="RH222_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV3798346.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            667..1242
                     /locus_tag="RH222_003383"
     CDS             667..1242
                     /locus_tag="RH222_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV3798347.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1284..2336)
                     /locus_tag="RH222_003384"
     CDS             complement(1284..2336)
                     /locus_tag="RH222_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV3798348.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2561..3481
                     /gene="lpxL"
                     /locus_tag="RH222_003385"
     CDS             2561..3481
                     /gene="lpxL"
                     /locus_tag="RH222_003385"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV3798349.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3653..4879
                     /gene="mdtG"
                     /locus_tag="RH222_003386"
     CDS             3653..4879
                     /gene="mdtG"
                     /locus_tag="RH222_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV3798350.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4962..5336
                     /locus_tag="RH222_003387"
     CDS             4962..5336
                     /locus_tag="RH222_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV3798351.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5337..5564)
                     /locus_tag="RH222_003388"
     CDS             complement(5337..5564)
                     /locus_tag="RH222_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV3798352.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5737..8280)
                     /gene="mdoH"
                     /locus_tag="RH222_003389"
     CDS             complement(5737..8280)
                     /gene="mdoH"
                     /locus_tag="RH222_003389"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV3798353.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8273..9808)
                     /gene="mdoG"
                     /locus_tag="RH222_003390"
     CDS             complement(8273..9808)
                     /gene="mdoG"
                     /locus_tag="RH222_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV3798354.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10202..11359
                     /gene="mdoC"
                     /locus_tag="RH222_003391"
     CDS             10202..11359
                     /gene="mdoC"
                     /locus_tag="RH222_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDV3798355.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11367..12848)
                     /gene="clsC"
                     /locus_tag="RH222_003392"
     CDS             complement(11367..12848)
                     /gene="clsC"
                     /locus_tag="RH222_003392"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV3798356.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12790..13323)
                     /gene="ymdB"
                     /locus_tag="RH222_003393"
     CDS             complement(12790..13323)
                     /gene="ymdB"
                     /locus_tag="RH222_003393"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV3798357.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13418..13729)
                     /locus_tag="RH222_003394"
     CDS             complement(13418..13729)
                     /locus_tag="RH222_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3798358.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13850..14182)
                     /gene="csgC"
                     /locus_tag="RH222_003395"
     CDS             complement(13850..14182)
                     /gene="csgC"
                     /locus_tag="RH222_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV3798359.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14241..14696)
                     /gene="csgA"
                     /locus_tag="RH222_003396"
     CDS             complement(14241..14696)
                     /gene="csgA"
                     /locus_tag="RH222_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV3798360.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14737..15192)
                     /gene="csgB"
                     /locus_tag="RH222_003397"
     CDS             complement(14737..15192)
                     /gene="csgB"
                     /locus_tag="RH222_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV3798361.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3878 a         3991 c         3695 g         4004 t
ORIGIN      
        1 taaaaataaa tatcattcaa tttgttattt ttgtgttgca attcattctc cgacacgcca
       61 ttttctttct ctcgcaaatt cgtattggat gcacttatgt cattcacaaa tacccctgaa
      121 cgctatggtg ttatatctgc ggccttccat tggctaagcg caattattgt ctacggcatg
      181 tttgctttag gtctgtggat ggtcacgctc agttattacg atggctggta tcacaaagca
      241 cccgaactgc ataaaagcat cggtattttg ttaatgatgg ggctggttat tcgcgttctg
      301 tggcgtgtca tatctccacc gccgggtccg ctgccgagtt attcgcccat gactcgtctt
      361 gccgcaaagg ctggacatct cgcattgtac ctattgctgt tcgccatcgg catcagcggc
      421 tatctgatct caactgccga tggtaaaccg atcagcgttt ttggctggtt tgacgtcccc
      481 gcgacccttg ccgacgctgg cgcacaggca gactttgccg gtgccctgca tttttggctc
      541 gcgtggagcg tcgtagtact gtccgttatg cacggattta tggccctgaa gcatcatttc
      601 atcgataaag acgacactct gaagcgcatg ctgggaaagt cgtcatctga ctatggagta
      661 taaaaaatga aaaaaagcct gcttggttta accttcgcgt ccctgatgtt ctctgccggt
      721 tcagcggttg ccgccgatta caaaattgac aaagaaggtc agcacgcctt tgttaatttc
      781 cgcatccagc accttggcta tagctggtta tacggcacct ttaaagattt cgacggtact
      841 tttacctttg acgaaaaaaa tccggctgcc gataaagtga atgtgacaat taacaccacc
      901 agcgtcgata ctaatcacgc cgaacgcgat aaacatcttc gcagtgcaga tttcctcaat
      961 accgcaaaat atccacaggc aacattcacc tccaccagcg tgaagaaaga cggtgacgaa
     1021 ctggatatta ccggcgatct gacgctgaat ggcgtaacca aacccgtcac gctggaagcg
     1081 aaattaattg gtcagggcga cgacccatgg ggtggtaaac gtgcaggctt cgaggccgaa
     1141 ggcaaaatta agctcaagga cttcaatatc aagacagatt taggtccagc ttctcaggaa
     1201 gtggatctga ttatttcagt ggaaggcgta cagcagaagt aagagtgaaa tgttgccgga
     1261 tggcacgcat ccggcaatga tatttattcc gttggatcag gaatgcccag tgttgtattc
     1321 agacgtccac gagacttatt aaagatctta ttgccatttt cacgtcctgc ccgacggcgt
     1381 cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa tctcactaca acaacctttg
     1441 tatttttccg cacatactgg acactgaata aacagcaggt ggcagccatc atttttacag
     1501 ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat gcgcgataat ctcatccgat
     1561 atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc caataaaacg caccggcaag
     1621 ccctgctcgc gcgccttacg ggcgtattca ataattccgc cctcgatatg ccagactttg
     1681 ttgaatccgt tatgtttcat ccaggcactg gccttttcac aacgaatgcc gccggtgcag
     1741 tacatgacga tttttttatc tttatgtgcc tgcatcatct cgactgcttt tggcagctgc
     1801 tcacggaagg tatctgccgg aatttcgagc gcgttttcaa agtgccccac ttcatactca
     1861 tagtggttac gcatgtcgat aaatagtgca tcgggatcgt caagcatggc gttcacttcc
     1921 gccgcttgca gatactcacc aacattgctg gcatcaaagt gaggatcgtc aataccgtcg
     1981 gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga aagatttccc gtcatcatcc
     2041 aacgcgatat tcaggcgtaa accctctaaa gccgggtcga aggcatagag ctgcgcgcga
     2101 aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt taatgccctc atgcgccaga
     2161 tacactcgcc caaaaacatt cagcgcggta aacagctgat ataaagcgtc acgggtcgcc
     2221 ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg tggttcgcgg ttcgctctca
     2281 gccaacattt tggcttttag cgcgtcgttg gaaatgcggt tgtgtaacac tggcatggtg
     2341 tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa ttatcgataa
     2401 ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt atttacaagc
     2461 gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa aatgcgacaa
     2521 tacatacaat tgcccgaata ggttgaaaaa caggattgat atgacgaatc tacccaagtt
     2581 ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg gcgtactttg
     2641 gttagtcgtg caattgccct acccggttat ctaccgcctc ggttgtggat taggaaaact
     2701 ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg aactgtgctt
     2761 cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg aatccgttgg
     2821 catgggcctg atggaaaccg gcatggcgtg gttctggccg gaccgccgaa tcgcccgctg
     2881 gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac gcggcatcct
     2941 gttagttggc atccattttc tgacactgga gctgggtgcg cggcagtttg gtatgcagga
     3001 accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc tacaaacctg
     3061 gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag gcatgattaa
     3121 agccctgaaa aaaggcgaag tggtctggta cgcaccggat catgattacg gcccgcgctc
     3181 aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg gaacctggat
     3241 gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta agccagatgg
     3301 caaagggtat caattgatta tgctgccgcc agagtgttct ccgccactgg atgatgccga
     3361 aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg caccagagca
     3421 gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt cacgctatta
     3481 aatctcccct gccggatgct tcagaatggc atccggcatt accacagcaa atccccctga
     3541 tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata attagtgtgc
     3601 ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg ctatgtcacc
     3661 ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct ggctaggctg
     3721 ttttcttacc ggtgccgcct tcagtctggt aatgcccttc ttacccctct acgttgagca
     3781 gcttggcgtt accggtcact ccgccctgaa tatgtggtcc ggtattgtct tcagcattac
     3841 atttttattt tcggccatcg cctcaccgtt ttggggtgga ctcgccgacc gtaaaggccg
     3901 aaaactcatg ctattacgct ctgccctcgg catgggcatc gtgatggtgt tgatgggact
     3961 ggcacaaaat atctggcagt ttttgatcct acgggcgctt cttgggttac ttggcggatt
     4021 tgtccccaac gctaatgctc ttatcgccac acaagtaccg cgtaataaaa gcggctgggc
     4081 gctgggtacg ctctccacag gcggcgttag tggtgcgttg ctcggcccaa tggctggcgg
     4141 cctgctcgcc gatagctacg gcttacgtcc ggtattcttt attaccgcca gtgtgctcat
     4201 actctgcttt ttcgtcaccc tgttctgcat cagagaaaaa ttccagccgg tcagcaaaaa
     4261 agagatgctg cacatgcggg aagtggtgac atcacttaaa aacccgaaac tggtactcag
     4321 cctgtttgtc accacgttaa tcatccaggt ggcgacaggc tcaattgccc ccattctgac
     4381 gctgtatgtc cgcgaactgg cgggtaacgt cagtaacgtc gcctttatca gtggcatgat
     4441 cgcctcggtg ccaggcgtgg cggctctgct aagcgcacca cgactcggca aacttggcga
     4501 tcgaatcgga cccgaaaaga tcctgattac agcgctgatc ttttctgtac tgctgttgat
     4561 cccaatgtct tacgttcaga cgccattgca acttgggatt ttacgttttt tgctcggtgc
     4621 cgccgatggt gcactactcc ccgccgtaca gacactgttg gtttacaact cgagcaacca
     4681 gatcgccggg cgtatcttca gctataacca atcgtttcgt gatattggca acgttaccgg
     4741 accattgatg ggagcagcga tttcagcgaa ctacggtttc agagcggtat ttctcgtcac
     4801 cgctggcgta gtgttattca acgcagtcta ttcatggaac agtctacgtc gtcgtcgaat
     4861 accccaggta tcgaactgat ttttcgcctt tcatacttgc aaaagcggag aatcagctat
     4921 ccttttccct gaaacctcat caactcaaag ggagaatcgt gatgaccatg tacgcaacgc
     4981 ttgaagaagc cattgacgct gcacgcgaag aatttcttgc agacaacccc ggcatcgacg
     5041 ctgaagatgc gaatgtgcaa cagttcaatg cccaaaaata cgttttgcag gacggcgaca
     5101 tcatgtggca agttgagttt tttgccgacg aaggggaaga aggtgaatgt ttacctatgc
     5161 ttagcggtga agccgcgcaa agtgtttttg atggcgacta tgatgagata gagatacgcc
     5221 aggagtggca ggaagagaat acattacatg aatgggacga gggggaattt cagcttgagc
     5281 caccgctgga taccgaggaa ggacgcgcag cagctgatga gtgggatgaa cgttaatcac
     5341 tcatacgggc catgatgaat gtcgatcggc agcagtaaag tatcaaaaac cagagagaat
     5401 ggcagatcaa ggatcgtgac ataacgccag gcggagtcac gcacatccca ttgcacaccg
     5461 ggataatatt ggttgccatg cccctgcccc ggtatagtgc gactaataat gctgccacag
     5521 ccactcagca aggtcaccat gatgctcacc acaattaaac gcatttgata cctctttcgt
     5581 aaaaaaatac cggcataacg ccggtatttt tattggctcg ttgtttttgc cggatgcggc
     5641 gtgaacgcct tatccgaccc acaaaatctt gcaaaatcaa taaattgcag gaacgatgta
     5701 ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg catccggttt
     5761 acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg aagaatatct
     5821 ctccggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat cgcttagcag
     5881 caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttcagcgcct gttcaacgtg
     5941 gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgcggtcg ccattgcggt
     6001 tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcat caagggagcg
     6061 ttgacgattc atctcaagga accgatcggt atcaaccagc acctgcggcg gcgaatactc
     6121 ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg cacggctgga
     6181 aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca gccagaacag
     6241 gaaacgcaga tccaaccacg ccataccaac agcccacact aaccctaaca gcagctgtga
     6301 gccgtggcgt ttgaacgctt caccccagga agtggagtca tcatcacgct gcggtgaatt
     6361 ccacaccact tcccagccaa ggaacgcgct gacgacgaag accgtatgga acagcatgcg
     6421 taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg taacgcgcca
     6481 gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca ataacttcgg
     6541 caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag gacgccactg
     6601 cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg tcaacgcatg
     6661 tactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag cggagagata
     6721 agacatcacg cccgtcagga ataccgcacg gtgaaccggg tgcataccct tcaccaggaa
     6781 cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta gctcatcaag
     6841 caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc agacccccca
     6901 acctgcacgg cgcatcaacg ccgcttccac gaagtcatgt gacaggattg aaccggcaaa
     6961 ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaacggtt tcacgcggat
     7021 aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca aaccggctgt
     7081 aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca gcgtatccat
     7141 accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt ccatcaggcg
     7201 caccagcccg cacaaacaat caccggtcat taccgagtca gcatccagca ccaccatgta
     7261 gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt tacgcttcac
     7321 gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag cgataagctc
     7381 catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac taagaatgta
     7441 gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg cacgcaggcc
     7501 agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg tgcgatgctc
     7561 cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat cgcgaccaat
     7621 aagcagttgc aggaagccca ttaacgccgt ccagaatccg gcggacaccc aacagaacag
     7681 taccgcaaag aggatcagga taccggtttg cagcatataa ggcagaagct gcataaagga
     7741 aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac cctgataagg
     7801 aagaatggtc ttcatatacc aggtcgcgac gacagtttgc gcgagcgtca ggatcaacag
     7861 aatgtaacgg cggatggtac cgacggtacg ccacttttgc tcgctctcct gctcttcttt
     7921 ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat cccagaaacg
     7981 gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg cttctggcat
     8041 cgccttcagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag caagtgaatc
     8101 tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat cctcccgcgc
     8161 ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag tcttcggcaa
     8221 tgccgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg tcttattcat
     8281 tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc accagcgcag
     8341 cacgcatttc agtggttttc ttggcatctt tcactttcac acgcatcacc agacgccagc
     8401 ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta tcaccaatgc
     8461 tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg ccggtaaaat
     8521 cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc ttcacatccc
     8581 ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt ttgtcttcat
     8641 cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc ggcagctgat
     8701 ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga atttccacca
     8761 gctcaacgct gcctttaccc cactcccctt tcggagtcac ccatgcgctt ggacgaagat
     8821 cgtaacgatc atcgagatct tcaaagcggg agaaatcacg accgcgctgc aacagaccga
     8881 agccttgcgg gttctccatc gagaagctgc tgaccgctaa atgtttcggg ttattcaacg
     8941 gacgccagat ccattcgcca ttaccggcat ggatagacag accgttagag tcgtgcaact
     9001 ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac atactggtta
     9061 acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac tgcacatcca
     9121 caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgcg cgcggcgagt
     9181 caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg atccagaact
     9241 ctttgaagcg tggaaattct tcacccgatg gcaaggcggt atcaattgcc aggccgcggg
     9301 cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc ccgagcatgc
     9361 tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta aaaccggcaa
     9421 aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg aaataatccg
     9481 ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg accggggtat
     9541 cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga ttgttccagt
     9601 acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcata tcgcggaaaa
     9661 cggagggcaa gttgcttttg ggcgcctcat agcctttccc ggctaaggat tgagcttgct
     9721 ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt aacattactg
     9781 cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt agtaagcact
     9841 tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag ccattccgac
     9901 aacagaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg attgcaaccg
     9961 ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag ggtttgccag
    10021 caattttatt tatcaaccgt cttacagacg cttccattct ggttagtggt agcgatgatg
    10081 ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc gctatggcaa
    10141 caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta gttaaccata
    10201 tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg cctggctgat
    10261 gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat ggcatgtgaa
    10321 tagcgccgaa ccatcattgt ggctgaccct ttttaatgac ttcatccact cgttccgcat
    10381 gcaggtattt ttcgttatat ccggctactt ttcctacatg ctttttttac gctatccctt
    10441 gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa cagccatccc
    10501 cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaaggaaaag cggaaagttg
    10561 gcctgggctg tcattgtatg acaaatataa tacgttggcc tgggaattaa tatcacacct
    10621 gtggttttta ctggtgttag tagtcatgac gacgctgtgc gtatggatat ttaagcgcat
    10681 cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg taaaactatc
    10741 ggtgattttt ttatgcctcg gcatcggtta tgcggtaata agaagaacga tttttattgt
    10801 gtatccgccc attctgagta atggcatgtt caattttatt gtcatgcaaa cgctgtttta
    10861 tttgccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta aagccttgtt
    10921 taccacgccg tctcgtggct gtacccttgc agcagcattg gcgtttgtcg cttatttact
    10981 caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg tgatcaccat
    11041 ggtcctcggt ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt tgcttaactt
    11101 ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc tggttcacca
    11161 cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca actggcttgg
    11221 ttttctctgt ggcctgatat ttgtagtagg gattgcgata attctgtatg aaattcattt
    11281 gcgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc gtgagaacga
    11341 taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa tatcgacgcc
    11401 agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag aataatctct
    11461 ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg gagctgccag
    11521 gccgcatcat actggctctg aataaagcgt ttatcaatta actgtgccag cgtttcgctc
    11581 tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa attgaaagag
    11641 ccgataaaca ccgttttacc atcgatgcta aaggttttag catgcaggct ggctccggaa
    11701 ttaccggtta tgccgcgatc gtgtaacgta ctactttgtt cacgcgtcgg cttgagttca
    11761 tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc ggcatggacg
    11821 acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac cccttttctc
    11881 accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga agagataata
    11941 tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa cagtgaatgc
    12001 cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt cttcgcccag
    12061 ataagcggca atgttccatc aaccagatga tttataaatg gactggattc cattttgcgt
    12121 aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat gcgatccgcc
    12181 atttcaccct ccgggacatc cagcacctgc tgtaagggtg aaaccgattt gcaataccag
    12241 tagcgggcga aatcatcggc aacgtcctct accacgggtc ctattgccat gacatctaaa
    12301 tccgaaaaaa gtggctcctc ccctgctcca aaataggcat caccaatatt tcgtcctccc
    12361 accagggtca ccacgccatc gacagtgaaa cttttattgt gcatacggcg attaagacgg
    12421 gaaaagtcgg tgatataacc aagcggacgc agcaagcgaa acgagaaagg attaaaaagc
    12481 cggacttcaa tgcgtggatg actgtcaagc aagcgtaaaa tgtcgtcaag tccgggcgta
    12541 ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc taacagggcg
    12601 gaaaacagta accgacccga catatcgtcc tgccagatgt aatactgaac atcgagcgta
    12661 tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact tttctccaga
    12721 ggaaaaaggc cacactgacc gggatgttgc gaacacagtg gcagcaccgc gctcgccagc
    12781 cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt gggcgttttc
    12841 ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa taaattctga
    12901 aacggttttt actgcgattt cagccgctgc cgcacgaggg taaccataaa ccccagtact
    12961 gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta ggctattgag
    13021 ataggcatcc tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga ctggcccgac
    13081 ggtgtgcact acggctttag cgggaagagc gcctgcaagc gtaataacgg catgccccgt
    13141 agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagtaggg ccggacccgc
    13201 tgcgcgatga atggccccat cgacgccgcc acctcccatt aatgaaggat tagccgcatt
    13261 cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa tacgcgtttt
    13321 cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt tggttaaatc
    13381 gcatttttct actcagcaac gtgagttgtt cagaaactta gttatattcg atgttcatta
    13441 cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt tgcgcgtttt
    13501 tgacattgcc gctcattaac gctttgctgg aataggtttt gcccggaaca gaaccattct
    13561 gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc ggttccacaa
    13621 tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcgatg gcaataaaag
    13681 gaaaaaacaa actgccgagc ataagagagt ttaaaaaagg tctgaacatt tattctattt
    13741 cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt ggctgattcg
    13801 cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat taagactttt
    13861 ctgaagaggg cggccattgt tgtgataaat gaagtgactg tccatcagaa acagtaacaa
    13921 ctattttcac ccgatcgtcc ggggaaatat ttaaactcaa cttcgtcaaa gcaatgggtt
    13981 gattagcagg caatgaaagg gtcttttctt gcttcgtctg actttgccct gaactgcctt
    14041 cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagtg acttcaggaa
    14101 taatggtata cacatcccct tgctgggtcg tattaaaggt tatctgactg gaaagtgccg
    14161 caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt gcgccctgtt
    14221 tctgtaatac aaatgatgta ttagtactga tgagcggtcg cgttgttacc aaagccaacc
    14281 tgagtcacgt tgacggagga gttagatgca gtctggtcaa ctgcagcacc gttgccacca
    14341 ccgaactgtt taaccgtcat ttcagaattt ttgccgttcc actgatcaag agtagcgctg
    14401 ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ccgagccctg accaacatct
    14461 gcaccattac cgccgccatg ctgggtaata gtcaagtcag agttacgggc atcagtttgc
    14521 agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctcaga atttgggccg
    14581 ctattattac cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc
    14641 agagcgctac cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa
    14701 aacccccatc ggattgattt aaaagtcgaa tggaaattaa cgttgtgtca cgcgaatagc
    14761 catttgcgac tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt
    14821 tgctttatta ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga
    14881 aatactggca tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt
    14941 ctggtcaatc tttgcccggt tgctactacc ttcttgcgca acaaccgcca aaagttttga
    15001 gcctccctgc cgtaactgag cactattatt cgtcccagct tgaccaatta tggctgcctg
    15061 attaaatgaa gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc
    15121 ataacctgct gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa
    15181 tttgtttttc atgttgtcac cctggacctg gtcttacatt taagaaatta aatcatttca
    15241 acttggttgt taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct
    15301 gcggaaatat ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa
    15361 agtattaaaa cccacgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctttta
    15421 aatctccgtt ttccgctatc aaaaatcacc agacagtcat ttttcttgcc cgtcgctgat
    15481 tgctgcgata tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaatt
    15541 gtccacggag gtgcggagaa aaaacaag
//
LOCUS       DAPHJE010000104        15427 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPHJE010000104 DAPHJE010000000
VERSION     DAPHJE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.105"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            101..619
                     /locus_tag="RH222_003398"
     CDS             101..619
                     /locus_tag="RH222_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV3798362.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            609..1835
                     /gene="dgcN"
                     /locus_tag="RH222_003399"
     CDS             609..1835
                     /gene="dgcN"
                     /locus_tag="RH222_003399"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV3798363.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1851..2333
                     /locus_tag="RH222_003400"
     CDS             1851..2333
                     /locus_tag="RH222_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3798364.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2409..2756)
                     /gene="rplS"
                     /locus_tag="RH222_003401"
     CDS             complement(2409..2756)
                     /gene="rplS"
                     /locus_tag="RH222_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV3798365.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2798..3565)
                     /gene="trmD"
                     /locus_tag="RH222_003402"
     CDS             complement(2798..3565)
                     /gene="trmD"
                     /locus_tag="RH222_003402"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV3798366.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3596..4144)
                     /gene="rimM"
                     /locus_tag="RH222_003403"
     CDS             complement(3596..4144)
                     /gene="rimM"
                     /locus_tag="RH222_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV3798367.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4163..4411)
                     /gene="rpsP"
                     /locus_tag="RH222_003404"
     CDS             complement(4163..4411)
                     /gene="rpsP"
                     /locus_tag="RH222_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV3798368.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4548..5909)
                     /gene="ffh"
                     /locus_tag="RH222_003405"
     CDS             complement(4548..5909)
                     /gene="ffh"
                     /locus_tag="RH222_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV3798369.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6076..6867
                     /locus_tag="RH222_003406"
     CDS             6076..6867
                     /locus_tag="RH222_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV3798370.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6888..8174
                     /locus_tag="RH222_003407"
     CDS             6888..8174
                     /locus_tag="RH222_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV3798371.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8229..8822)
                     /gene="grpE"
                     /locus_tag="RH222_003408"
     CDS             complement(8229..8822)
                     /gene="grpE"
                     /locus_tag="RH222_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV3798372.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            8945..9823
                     /gene="nadK"
                     /locus_tag="RH222_003409"
     CDS             8945..9823
                     /gene="nadK"
                     /locus_tag="RH222_003409"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV3798373.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9909..11570
                     /gene="recN"
                     /locus_tag="RH222_003410"
     CDS             9909..11570
                     /gene="recN"
                     /locus_tag="RH222_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV3798374.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11719..12060
                     /gene="bamE"
                     /locus_tag="RH222_003411"
     CDS             11719..12060
                     /gene="bamE"
                     /locus_tag="RH222_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV3798375.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12122..12412)
                     /locus_tag="RH222_003412"
     CDS             complement(12122..12412)
                     /locus_tag="RH222_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV3798376.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12402..12839)
                     /gene="ratA"
                     /locus_tag="RH222_003413"
     CDS             complement(12402..12839)
                     /gene="ratA"
                     /locus_tag="RH222_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV3798377.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13010..13492
                     /gene="smpB"
                     /locus_tag="RH222_003414"
     CDS             13010..13492
                     /gene="smpB"
                     /locus_tag="RH222_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV3798378.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13707..14069
                     /gene="ssrA"
                     /locus_tag="RH222_003415"
     tmRNA           13707..14069
                     /gene="ssrA"
                     /locus_tag="RH222_003415"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14254..>15427
                     /locus_tag="RH222_003416"
     CDS             14254..>15427
                     /locus_tag="RH222_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3798379.1"
                     /translation="MARTTRPLTNTEVLRAKALEKDLTLHDGDGLFLIVKTSGKKLWR
                     FRYQRPATKQRTMMGLGAFPALSLADARGLRADYLALLANGIDPQIQAEVAEEQQQIA
                     LDSIFSMVAANWFQLKSKSVTPDYAKDIWRSLEKDVFPAIGEIPVQQIKARILVEALE
                     PIKARGALETVRRLVQRINEIMIYAVNTGLIDANPASGIGMAFEKPKKQNMPTLRPEE
                     LPKLMRSLVMSNLSVPTHCLIEWQLLTLVRPSEASGARWAEIDLDTKLWTIPAERMKA
                     KREHIVPLSPQALEILDVMKPISAHREHVFPSRNDPKQPMNSQTANAALKRIGYGGKL
                     VAHGLRSIASTALNESGFNSDIIESALAHIDKNEVRRAYNRSIYLEQRRELMSWWGI"
BASE COUNT         3902 a         4052 c         3658 g         3815 t
ORIGIN      
        1 atttctcaca ttgatgacga tcgcatgaaa aaatgatatt ttaatgagca ttctgaaaaa
       61 atatcgatgc atttcgagcg aagatggtgg ggatccctga atgcgttttt ctcaccgact
      121 gttcttactt ctgattctcc tgctgacggg agcaccaata ctcgctcagg agccttctga
      181 cgttgcgaaa aacgtgcgta tgatggtttc cggtattgtc agttacaccc gctggcctgc
      241 gctatcaggg ccaccaaagt tatgtatctt ttcatcttca cgttttagta ccgccctaca
      301 agagaatgcc gcgacatctt tgccttatct gcctgtcatc attcacactc aacaagaggc
      361 gatgatttca ggctgcaatg gtttttattt tggcaacgag tcaccgacat tccagatgga
      421 attaaccgaa caatatccgt caaaggcgtt attattaatt gccgaacaaa ataccgagtg
      481 cattattggt agcgcctttt gtctgatcat ccacaataat gacgtcagat ttgccgtaaa
      541 cctggatgcc ttatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa tgctcgcacg
      601 gaagaaaaat gatggataac gataattctc ttaataagcg ccccacgttt aaaagagcat
      661 tacgcaacat cagtatcacc agcatattta tcactatgat gctgatctgg ttgctgcttt
      721 ccgtgacctc ggtgctgacc ctgaaacagt acgcgcaaaa aaacctggca ctgacagcag
      781 caacaatgac ttacagtctg gaagcagctg tcgtttttgc cgatggccct gcagcaactg
      841 aaacactggc agcgctgggc cagcaagggc aattttcaac tgcagaagta cgtgataagc
      901 agcaaaatat tctggcgtcc tggcattaca cccgtaagga tccaggcgat actttcagca
      961 atttcataag ccactggctc ttccccgccc ccatcattca gccgattcgt cacaatggtg
     1021 aaaccattgg cgaagtacgc ttaaccgctc gcgacagttc aatcagccat tttatctggt
     1081 tttcgctcgc cgtactgacc ggttgtattc tgctggcatc aggcatcgca attaccctca
     1141 cccgccattt gcacaatggc ctggtggaag cactgaaaaa tatcaccgat gtcgtacatg
     1201 atgtgcgttc caaccgcaat ttttcccgac gagtttcgga agaacgtatc gctgagtttc
     1261 accgcttcgc tctcgacttc aacagtctgc tggatgaaat ggaagagtgg cagcttcgtt
     1321 tacaggctaa aaatgcgcag cttctacgta ccgcgctaca tgacccatta accgggctgg
     1381 ctaaccgcgc agcgtttcgt agcggcatca acacgttgat gaacaattcc gatgcccgaa
     1441 aaacgtcggc gttactattt cttgatggcg ataatttcaa atacatcaat gatacctggg
     1501 gtcatgcgac gggcgataga gtcttgattg aaatcgcaaa acggttagct gaagttggcg
     1561 ggctgcgaca taaagcatac cgcctgggcg gcgatgaatt cgctatggtg ctctatgatg
     1621 tacagtcaga atctgaagtg cagcagatat gctcagcact gacacaaatc tttaatctcc
     1681 cgtttgatct tcataatggt catcagacca ccatgacatt aagcattggt tacgcgatga
     1741 ccattgagca cgcctctgcg gaaaaattac aagagcttgc cgatcacaat atgtatcagg
     1801 ccaaacacca gcgtgccgaa aagctggtga gataacaagg atatatcgat atgataaagc
     1861 acctggtagc acccctgatt ttcacctcac taatactgac tggctgccag tcccctcagg
     1921 gaaagtttac tcctgagcaa gtcgccgcta tgcaatctta tggatttact gaatccgccg
     1981 gcgactggtc gctgggctta tcagatgcca ttctgttcgc aaaaaatgac tacaaattgc
     2041 tcccggaaag ccagcaacag atccaaacca tggcagccaa attggcctcg acagggctaa
     2101 cacatgcccg tatggatgga cacaccgata actatggtga agacagttac aacgaaggct
     2161 tatcattgaa acgggcgaat gtcgtggccg atgcatgggc tatgggtgga caaattccac
     2221 gcagcaatct caccacacag ggtttaggaa aaaaatatcc catagccagt aacaagaccg
     2281 cccagggccg cgccgagaac cgccgcgtcg cagtggtgat tactacccct taaaacacca
     2341 gcaaacagat aaaaaagggc cagccaattg gccagccctt cttaacagga tgtcgcttaa
     2401 gcgaaatctt agttaagacg ctctttgata cgagcagcct taccagtacg ctcacgcagg
     2461 tagtacagtt tagctttacg aacagcacca cgacgtttga cagaaatgct gtcaactacc
     2521 ggagagtgag tctggaagac acgctcaacg ccttcgccgt tggaaatttt acgaacagtg
     2581 aatgcagagt gcagaccgcg gttacgaata gcgataacca cgccctcgaa tgcctgcaga
     2641 cgttttttgg aaccttcaac aacccatact ttcacttcca cggtatcacc cggacggaag
     2701 gaaggtacgt cctgcttcat ctgctcttgt tcaagttgct taataatgtt gctcataatt
     2761 taatctctta tcctgggtaa actgatatct cgggggctta cgccatccca tcatgtttat
     2821 gttgctgttg tgcgtgttcc gttttgaact ccgccagcaa ccttgcttgc tcttcagtca
     2881 gagccaggtt ttccagaagt tcaggtcttc taagccaggt acggcccagc gactgtttca
     2941 aacgccagcg acgtatctcg gcatggttgc ccgacagtaa cactggcgga acttccatcc
     3001 cttctaacac ctcaggccgc gtatagtgcg ggcaatccag caatccttca gcaaaggaat
     3061 cttccgttgc cgaggcttca tgtcccagta ctcccggaat aaaccgggaa acggagtcaa
     3121 tcagcgtcat tgctggtaac tcaccaccac tgagaacgta atcgccgatt gaccattctt
     3181 cgtcaatttc ggtttggatc acgcgctcat ctataccttc gtagcgaccg cacaccagaa
     3241 tcaatttttg attcgttgcc agttcgctga cgcccgcttg atcaagcttg cgtccctgtg
     3301 gtgacagata aatcaccttt gcgccttcac ccgccgcggc ttttgctgca tgaatggcgt
     3361 cccgcaaggg ttgcaccatc attaacatcc ccggtccgcc gccgtaagga cgatcgtcca
     3421 cggtacggtg ccggtcatgc gtgaagtcgc gaggactcca gctctggatg ctcagcaggc
     3481 catttttaac tgcccggcca gttaccccgt aatcggtaat tgcgcggaac atttcaggaa
     3541 acaggctaat tatgccaatc cacatagcgc cgtcttttac cgtttatccg gtggtttaaa
     3601 aaccaggatc ccaatctact tcgattgaac gagtagtgag atcgactttc ttgataacct
     3661 gcccatcgag gaacggtacg agacgttcct tgataccaaa cgcatctttc aggtttgcct
     3721 taatgacgag aacgtcatta gatccggttt ccatcatatc gacgacttta ccgagatcgt
     3781 agccttcagt ggttactacc tggcagccca tcaggtcttt ccagtagtag tccccctctt
     3841 caagctgagg cagctgcgat gaatccacga caatttcaca attcgtcagc aggttcgccg
     3901 catcacgatc gtcaacgcct ttcagcttga tgatcatgtc ctgattgtgg tgcttccagc
     3961 tttccagctg gacttgctgc cactgacccg ccttctggat aaaccagggc tgatagtcaa
     4021 aaatgctttc ggcgtcttcg gtggaagaaa acactctgag ccacccacga ataccgtaag
     4081 acgaacccat ttttcccaaa acgatgggat caacaggtgc ttgcgcggtg agttgtttgc
     4141 tcatcatgac caccgtgaca gattaagctg ctttgtttac ttctttgatc agcgcagcaa
     4201 cgcgatcaga aatagttgcg ccctggccaa cccagtgagc gatgcgatcc agatccaggc
     4261 gagtgccttc ttctttttcg ctagcgattg ggttgaagaa accaacgcgc tcgatgaagc
     4321 gaccgttgcg tgcattacgg ctgtcagcga caacaacctg gtagaacgga cgctttttag
     4381 cgccgtgacg tgctaaacga atagttacca taacatcctc ttgtgtgaat aaaacaaccg
     4441 gaccccatcg aggaacggag tccggtgtca tattaaaagc ccgaaaattt tactgatttc
     4501 tggagaaaat gcaatcagca gttaataact ctgtcgcata aggtcattta gcgaccagga
     4561 aatcccggcg gcatcatccc cttcatgctt ctcatcatct tcgccattcc gcccttcttc
     4621 attttcttca tcatgcgctg catgtcgtcg aactgcttca gaaggcggtt aacgtcctgc
     4681 acctgcatac cgcaaccggc agcgatacga cgcttacgcg aacctttgat gatttctggc
     4741 ttagcgcgct ctttcatcgt catcgagttg atgatggctt ccatacgcac cagcacttta
     4801 tcgtccatct gcgacttaac attatccggg atctgcccca tgcccggcag cttgcccatc
     4861 aggctagcca tgccgcccat atttttcatc tggcgcagtt gctcaagaaa atcgttgaga
     4921 tcgaagccgt cgcctttttt cagcttgctg gctaatttct ctgcctgcgc gcggtcaact
     4981 ttgctttcga tatcttcgat cagcgacagt acgtcgccca tgccgagaat acgcgacgcg
     5041 atgcggtccg gatggaacgg ctccagcgcc tcagttttct cgccaacacc gagaaacttg
     5101 atcggtttgc cagtgatgtg acgaatagag agcgccgcac cgccgcgggc atcgccgtcc
     5161 actttggtca acactacgcc ggtaagcggt aacgcttcat tgaatgcttt tgccgtattg
     5221 gccgcatcct gaccggtcat ggcgtcaacc acaaacaggg tttcaaccgg gttaatcgac
     5281 gcatggactt gtttgatctc gtccatcatc gcttcgtcaa cgtgcagacg accagcggta
     5341 tccaccagca gcacgtcgta gaatttcagt ttggcttctt tcagcgccgc gttaacgata
     5401 tctaccggct tctgaccaac atcagaaggg aagaaatcaa cgcccacctg ctctgccagc
     5461 gtctcaagct gtttgattgc cgccgggcga taaacgtcgg cagaaaccac cagcactttc
     5521 ttcttgtgct tctcgcgcag gaacttaccg agtttaccaa cgctggttgt tttaccggca
     5581 ccttgcaggc ccgccatcag cacgaccgca ggcggttgcg ccgccaggtt cagggtctgg
     5641 ttctcttcgc ccatcgccgc aaccagttcg ttacggacga ttttgacgaa ctcctgcccc
     5701 ggcgtcaggc tcttattaac ttcatgacca accgctttct cttttacgcg attgataaac
     5761 tcacgcacta ccggcagagc gacgtccgcc tccagcagcg ccatgcgcac ttcgcgcagc
     5821 gtatctttta cgttgtcttc agtgaggcgt ccacggccac tgatattgcg cagcgtgcgc
     5881 gacaaacgat cggttaaatt atcaaacatt gtctctcgcc tggggtggaa acggttggcc
     5941 gcaatcgcga cacatcatca gtattttgcc gcagtataac atgaaggcgt ctttgttgtt
     6001 atgcaacggt tggagcagcg ttcacctgac gctatactgc ttctctttct tattgctcaa
     6061 actgtcgaca ttactatgcc cgtttttgct ctgctcgcgc ttgtcgccta ctccgtcagt
     6121 cttgcgctga ttgttcccgg tctgctgcaa aaaaacggcg gctggcggcg catggctatt
     6181 atttctgcgg tcattgcgct ggtctgccac gcaatcgctc tggaagcccg catcctgccc
     6241 gacggtgata gcggacaaaa cctcagcctg ctgaacgttg gttcattggt cagtttgatg
     6301 atctgtacgg taatgaccat tgtggcttct cgcaatcgtg gctggttgct gctacccatt
     6361 gtctatgcct ttgcgcttat caacctggcg ctggcgacct tcatgcccaa tgaatacatc
     6421 acccatctgg aagcaacgcc tgggatgctg gtgcacattg gcttatcgct cttttcctat
     6481 gccacgctaa ttatcgccgc cctgtacgcg ctgcaactgg cgtggattga ttaccaactg
     6541 aagaacaaga agctggcatt taaccaggaa atgccgccat tgatgagtat cgagcgtaaa
     6601 atgttccaca tcacgcagat tggcgtggtg ctgctaacgc tcacgctttg cactggcctg
     6661 ttctacatgc acaacctatt tagcatggaa aatatcgaca aggctgtgct ctctatcgtg
     6721 gcgtggtttg tctatattgt gctgctgtgg ggacattacc atgaaggatg gcgtggacgc
     6781 cgcgtcgtct ggtttaacgt tgcgggcgcg gtaattctga cactggccta cttcggcagc
     6841 cgaattgtcc agcagttaat cagctaaacc cagaaaagga gtttcccctg gaacacattt
     6901 ctactactac gttgatcatt attctgatca tcatggtggt catttcagcc tatttttccg
     6961 ggtccgaaac cggaatgatg accctcaacc gctatcgtct gcgacatatg gcgaaacagg
     7021 gtaatcgctc ggccaaacgc gtcgaaaaat tgctgcgtaa gccagaccgc ctgataagcc
     7081 tggtgttaat cggcaataac ctggtcaata ttcttgcctc cgcgctcggc actattgttg
     7141 ggatgcgttt gtacggcgat gcgggcgtgg caattgcgac tggtgtgctg acttttgtcg
     7201 tactggtatt tgctgaggta ttgccgaaaa ccattgccgc gctgtacccg gaaaaagtcg
     7261 cttatccgag tagttttctg ctggctccgc tgcaaatttt gatgatgccg ctggtctggt
     7321 tgctgaatgc tatcacccgt atgctgatgc gcatgatggg tatcaaaacc gatatcgtgg
     7381 ttagcggctc tttgagcaaa gaagagttgc gcactatcgt gcacgaatca cgctcacaaa
     7441 tttcccgtcg caatcaggat atgctgctgt cggtgctcga tctggaaaaa atgaccgttg
     7501 atgacatcat ggtgccgcgc agtgaaatta tcggtattga tatcaacgat gactggaaat
     7561 cgattctgcg ccaactctcc cactcacctc acgggcgcat cgtgctctac cgtgattcgc
     7621 tggacgacgc catcagtatg ctgcgagtac gtgaagcctg gcggttgatg tcggagaaaa
     7681 aagagttcac caaagaaacc atgctgcgcg ccgcggacga gatctatttt gtgccggaag
     7741 gtacgccgct cagcacgcag ttggtaaagt ttcagcgcaa caaaaagaaa gtcggcctgg
     7801 tcgtcaacga gtatggagac attcaggggc tggtgacggt tgaagatatt ctggaagaga
     7861 ttgtcggcga tttcactacg tcgatgtcgc caacacttgc cgaagaggtc acgccgcaaa
     7921 acgacggttc ggtgattatc gatggcaccg ccaacgtgcg ggaaatcaac aaagccttta
     7981 actggcatct accggaagat gatgcccgca cggttaatgg cgtcattctt gaggcactgg
     8041 aagagatccc tgtcgcaggc acccgcgtgc gtattggcga gtacgatata gatattctcg
     8101 acgtacagga caatatgatt aagcaggtaa aagtttttcc tgtgaaaccg ctgcgcgaga
     8161 gtgtggcgga gtaacgaaaa cggccctgca ttcgcatgca gggccgtgaa ttattacgaa
     8221 aacagaaatt acgcttttgc tttcgctaca gttaccatcg ccgcacgaat cgtacgacca
     8281 ttcagcgtat aacccttctg cataatgccc agtacgttac ctggcgcaac gtcatcagat
     8341 tccaccattg cgatagcctg atgcacattc gggtccagcg gcacgttagt ttcggcgatc
     8401 acttcaacac caaacttacg cacaacatcc agcatcgact tcagcgtcag ctcaatgcct
     8461 tcaaccatcg cggacatatc cgggttagct ttatcagcca cttccagcgc acgatccagg
     8521 ctatcaatca ccggcagcaa ttcgttgatg aatttctcca gcgcgaattt gtgggctttt
     8581 tcaatatcca gttcagtacg tcgacgcagg ttttccattt cggcttttac acgcaaaatg
     8641 ccgtcacgtt cacgggtctg ggcttcagcc agctgagctt cgagattcgc aattttttca
     8701 tcgcgcgggt ccacctgctc agcagaagct tctggctcaa ctgcctcaat ctcttcgtgc
     8761 tgatccatga taatttcttc cggggcttgc ccctcaggcg ttttctgttc tttactactc
     8821 atgaatttct ccgcgttttt ttcgcattca tctcgctaac ttcgcttatt atggggatca
     8881 gtttcagggt ttcaagggaa gcactcacat tgtcatcaat cttcgcaaca aggacctcgg
     8941 aaaaatgaat aatcatttca agtgtattgg cattgtggga cacccacggc accccactgc
     9001 actgacaaca catgaaatgc tctaccgctg gctgtgcaca aaaggttacg aggtcatcgt
     9061 tgagcaacaa atcgctcacg aactgcaact gaagaatgtg aaaactggca cgctcgcgga
     9121 gattgggcaa ctagctgatc tcgcggtagt cgttggtggc gacggtaata tgctgggcgc
     9181 ggcacgcaca ctcgcccgtt acgatattaa agttattgga atcaaccgtg gcaacctggg
     9241 tttcctgact gaccttgacc ccgataacgc ccagcaacag ttagccgatg tgctggaagg
     9301 ccactacatc agcgagaaac gttttttgct ggaagcgcaa gtctgtcagc aagattgcca
     9361 gaaacgcatc agcaccgcga taaatgaagt ggtgcttcat ccaggcaaag tggcgcatat
     9421 gattgagttc gaagtgtata tcgacgagat ctttgcgttt tctcagcgat ctgatggact
     9481 aattatttcg acgccaacag gctccaccgc ctattccctc tctgcaggcg gtcctattct
     9541 gaccccctct ctggatgcga ttaccctggt gcccatgttc ccgcatacgt tgtcagcacg
     9601 accactggtc ataaacagca gcagcacgat ccgtctgcgt ttttcgcatc gccgtaacga
     9661 cctggaaatc agttgcgaca gccagatagc actgccgatt caggaaggtg aagatgtcct
     9721 gattcgtcgc tgtgattacc atctgaatct gattcatccg aaagattaca gttatttcaa
     9781 cacattaagc accaagctcg gctggtcaaa aaaattattc taattttacg ccagcctctt
     9841 tactgtatat aaaaccagtt tatactgtac acaataacag taatggtttt tcatacagga
     9901 aaacgactat gttggcacaa ctgaccatca gcaactttgc tatcgttcgt gagcttgaga
     9961 ttgattttca tagcggcatg accgtaataa ctggcgagac cggcgcgggt aaatctattg
    10021 caatagatgc cctcggtctt tgtctcggtg gtcgcgctga agccgacatg gtgcgtaccg
    10081 gcgctgctcg cgctgacctg tgcgcccgtt tttctctgaa agatacgcca gcggctctgc
    10141 gctggctgga agaaaaccag cttgaagacg ggcatgaatg tttgcttcgt cgcgtgatca
    10201 gcagcgatgg tcgctcccgt ggtttcatca acggtacagc tgttcctctg tcacaactgc
    10261 gcgaactggg tcagttgctg attcagatcc atggtcagca cgctcatcag ttactcacca
    10321 aacccgagca ccaaaagttc ctgcttgatg gctatgccaa tgaaacctct ctactccagg
    10381 aaatgacagc acgttatcag ttgtggcatc aaagctgccg tgacctcgcg catcatcaac
    10441 agttaagtca ggaacgcgct gcccgtgcgg aactgctgca ataccaatta aaagaactta
    10501 acgaatttaa tccgcagccc ggagagtttg aacaaatcga cgaagagtac aaacgtctgg
    10561 cgaacagcgg tcaattgctg accaccagcc agaatgcatt ggcattaatg gccgacggtg
    10621 aagacgcaaa cctgcaaagt cagctttaca cggctaaaca actggtgagc gaattgattg
    10681 gcatggacag caaactgtcc ggcgtacttg atatgctgga agaagctacc atccagattg
    10741 ctgaagccag cgatgaactg cgccactact gcgatcgtct ggatctcgat cccaaccgac
    10801 tatttgaact tgaacagcgc atctcaaaac agatttcgct ggcacgtaaa catcacgtca
    10861 gccctgaggc attgccacag tattaccagt cgctactgga agaacagcag caactggacg
    10921 atcaggccga ctcacaagaa acgcttgcgc tggcggtaac gaaacatcat cagcaggcac
    10981 tggaaatcgc gcgcgcatta caccaacaac gccagcaata tgcagaagaa cttgcacagc
    11041 tgatcaccga cagtatgcat gcgctctcaa tgccgcatgg gcagtttacg atcgatgtta
    11101 aatttgacga gcatcacctg ggcgctgacg gtgccgatcg tattgagttt cgggtaacca
    11161 ccaacccagg tcagccaatg cagcctattg ccaaagtcgc atccggtggt gaattgtccc
    11221 gcatcgcact ggcaatccag gtcatcacgg cgcgtaaaat ggaaaccccg gcactgattt
    11281 ttgatgaagt ggatgtaggg attagcggtc caacagcggc agttgtcggc aaactgctgc
    11341 gtcaactcgg cgaatcaact caggtgatgt gtgttaccca cctgccacaa gtcgcgggat
    11401 gtggtcatca acactatttt gtcagcaaag aaaccgatgg tgcgatgaca gaaacgcata
    11461 tgcaatccct gaataaaaaa gcgcggttac aagagctggc gcgcctgctt ggtggcagtg
    11521 aagtcacacg taatacactg gcgaatgcga aagaactgct tgcagcgtaa acttttttcc
    11581 tgcttcacgg tcagagtaaa cagcaaaacg ccgtaagacc ggaaagcaaa aggttttaaa
    11641 gtgatgaaag gtctattatc atcggcatat tacagatgag ccacgtactg ctcgggcccg
    11701 aaaaggaatc aaatcactat gcgctgtaaa acgctgactg ctgcagcagc agtactattg
    11761 atgttgaccg caggctgttc cactctggag cgagtggttt accgtcctga catcaaccag
    11821 gggaactatc tgaccgctaa cgacgtatcc aaaatacgtg ttggcatgac gcaacaacaa
    11881 gttgcgtacg cattgggtac accgctgatg tccgatccat ttggtacgaa tacctggttc
    11941 tatgtcttcc gccagcaacc aggtcatgaa ggtgtaacgc agcaaacgct gacgctgacc
    12001 tttaacagta gcggtgtgtt gaccaatatt gataacaaac ctgcgctgag tggtaactaa
    12061 taataaagtt gctctcaaag acgttaaaaa aggtgctcaa tgagcacctt ttttctgtct
    12121 gttatttatt cgctgatttt tctgctcgtt gcctgcgaag ctctttcgga tcggcaatga
    12181 gagggcgata aatctccacc cgatcgccat catgcacact atcgcttagt tttgccggac
    12241 ggctgtaaat gccgactttg tttttagtta aatcgatatc ggtacgcaat tccagcaagc
    12301 cactggcgcg aatagcttct tcaaccgtcg cgccctcctg cagcgtcact cgctgcaggt
    12361 actgcttctc aggtagcgca taagccacct caacggcaat tttacctggc actgtaaacc
    12421 tctttcgcac gaaccgtaaa agcctggacc atattagccg ccagctcttt aaacacgcga
    12481 ccaaaggcga gttcaatcaa cttattggta aactcaaagt cgagatgaaa ctcgatacga
    12541 cacgcctcct ggctcagcgg cgtaaacttc catccaccaa tcaatttctt gaacggccca
    12601 tccaccagat tcataagaat actttggtta ctggtcaact ggttgcgggt agtaaacgtt
    12661 ttgctgatcc cagccttaga gacatctacc gccgcagtca tctgcccagg agtggactcc
    12721 agaatccgac ttccggtaca acccggcaaa aactgaggat aagactgaac gtcattcact
    12781 aactgataca tttgctccgc gctgtagggt accagtgcgg tccggctaat ctgaggcata
    12841 acaatttcca tcaacaaaaa tccaacaaat aatatcattt atcctgctgt aaaaaaaacg
    12901 ctatcccggc gctgggtaac atcgggttca tgctaagata gagccttgtc ccccgcagga
    12961 ttgatatggg gtgttttcga tttcagatta ccgatgattc acgacgctta tgacgaagaa
    13021 aaaagcacat aaacctggtt cagcgaccat cgcgcttaac aagcgcgccc gtcacgaata
    13081 ctttatcgaa gaagagttcg aagcgggact tgccctgcaa ggctgggagg ttaaatccct
    13141 gcgcgcagga aaagccaata tcagcgacag ctacgtcctt ctgcgtgacg gagaggcatt
    13201 tctgtttggc gctaacatca cgccaatggc cgtggcctcc acgcatgtgg tgtgcgatcc
    13261 tacccgtacc cgcaagttac ttctcaacca acgcgaactg gactcattgt acggtcgcgt
    13321 caatcgagaa ggctataccg tagtggcgct ctccctgtac tggaaaaatg cctggtgcaa
    13381 agtgaaaatc ggcgtcgcca aaggtaaaaa acagcacgat aaacgttcag atatcaaaga
    13441 acgcgaatgg caggtggata aagcacgtat catgaaaaac gcccaccgtt aaacctgcac
    13501 tccaattatt gaccagttcc tcaccgcgcc tccctctccg gcggcgcgaa tgaacatctt
    13561 attggctatc acatccgaca caaatgttgc catcccattg cttaatcgaa taaaaatcag
    13621 gctacatggg tgctaaatct ttaacgataa cgccattgag gctggtcatg gcgctcataa
    13681 atctggtata cttaccttta cacattgggg ctgattctgg attcgacggg atttgcgaaa
    13741 cccaaggtgc atgccgaggg gcggttggcc tcgtaaaaag ccgcaaaaaa tagtcgcaaa
    13801 cgacgaaaac tacgctttag cagcttaata acctgcttag agccctctct ccctagcctc
    13861 cgctcttagg acggggatca agagaggtca aacccaaaag agatcgcgtg gaagccctgc
    13921 ctggggttga agcgttaaaa cttaatcagg ctagtttgtt agtggcgtgt ccgtccgcag
    13981 ctggcaagcg aatgtaaaga ctgactaagc atgtagtacc gaggatgtag gaatttcgga
    14041 cgcgggttca actcccgcca gctccaccaa atcatgatcc ggatacgtcc ggtgaagtac
    14101 agaaagcccg catggcacaa gccctgcggg cttttttgtg tctgtcgttg tccgagaaca
    14161 tccggctaaa tccggtgatt attggtatac gtttaggtat acggtaggat gtatacctaa
    14221 aagcgtatac caattcatga aggagcggcc acagtggcac ggacaacacg cccccttacc
    14281 aacaccgaag ttcttcgcgc taaagcgtta gagaaggatc taactctgca tgatggcgat
    14341 ggccttttcc tgatagtgaa aaccagcggg aaaaagctct ggcgtttccg ttatcaacgt
    14401 ccagcgacaa aacagcggac aatgatgggg cttggtgctt tccccgccct ttcacttgct
    14461 gacgcccgag ggttaagagc ggattacctt gccttgttag ccaacggaat tgacccacaa
    14521 attcaggccg aagttgcaga ggaacagcag caaatcgctc tggacagtat tttctcaatg
    14581 gtcgccgcta actggttcca gctcaaaagt aaaagcgtta cccctgatta cgcaaaagac
    14641 atttggcgct cattggagaa agatgtattc cctgccatcg gtgagatccc cgttcagcaa
    14701 attaaagccc gaatattagt tgaagcattg gaaccaatca aagctcgtgg agcgcttgag
    14761 actgtacgtc gactagtgca gcgtattaac gagataatga tttatgccgt aaacactggt
    14821 ctgattgatg ccaatccagc atcaggtatt ggaatggcct ttgagaagcc caaaaaacaa
    14881 aacatgccga cgctgcggcc agaagaattg ccgaagctga tgcgttcttt agtcatgtca
    14941 aatctgtctg ttccgactca ctgtctcatt gaatggcaac tcctgacgct cgtgcgccct
    15001 tctgaggcct ctggtgcccg ttgggcagag atcgatctcg atacgaagct ctggactatt
    15061 ccagccgaac ggatgaaagc taagcgggaa cacattgttc ctttatcacc acaggcatta
    15121 gagattctgg atgtaatgaa gccaattagc gctcaccgag aacatgtttt tcccagcagg
    15181 aatgatccta aacaacctat gaatagccaa actgctaatg ctgctcttaa aaggattggt
    15241 tatggcggta aactcgttgc tcatgggctt cgttctatag ctagcacagc gttgaacgag
    15301 tctggtttta attccgatat tatagaatca gctttagcgc atatagataa aaatgaagta
    15361 cggcgggcgt ataatcgttc tatttattta gaacaacgca gagaattgat gtcatggtgg
    15421 ggtataa
//
LOCUS       DAPHJE010000105        15235 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPHJE010000105 DAPHJE010000000
VERSION     DAPHJE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.106"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            60..332
                     /locus_tag="RH222_003417"
     CDS             60..332
                     /locus_tag="RH222_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV3798380.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKTRQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(333..1205)
                     /gene="rluF"
                     /locus_tag="RH222_003418"
     CDS             complement(333..1205)
                     /gene="rluF"
                     /locus_tag="RH222_003418"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV3798381.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1553..2500
                     /locus_tag="RH222_003419"
     CDS             1553..2500
                     /locus_tag="RH222_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDV3798382.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2570..3376
                     /locus_tag="RH222_003420"
     CDS             2570..3376
                     /locus_tag="RH222_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3798383.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSKRASYITGVTTNIAGGKTRG"
     gene            3386..3793
                     /locus_tag="RH222_003421"
     CDS             3386..3793
                     /locus_tag="RH222_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDV3798384.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            3793..4287
                     /locus_tag="RH222_003422"
     CDS             3793..4287
                     /locus_tag="RH222_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDV3798385.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4353..5150
                     /locus_tag="RH222_003423"
     CDS             4353..5150
                     /locus_tag="RH222_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDV3798386.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5161..5985
                     /locus_tag="RH222_003424"
     CDS             5161..5985
                     /locus_tag="RH222_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDV3798387.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6037..7266
                     /gene="sorE"
                     /locus_tag="RH222_003425"
     CDS             6037..7266
                     /gene="sorE"
                     /locus_tag="RH222_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDV3798388.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADVAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7649..8338
                     /gene="pepE"
                     /locus_tag="RH222_003426"
     CDS             7649..8338
                     /gene="pepE"
                     /locus_tag="RH222_003426"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV3798389.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8429..10060)
                     /locus_tag="RH222_003427"
     CDS             complement(8429..10060)
                     /locus_tag="RH222_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV3798390.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10280..13963)
                     /gene="metH"
                     /locus_tag="RH222_003428"
     CDS             complement(10280..13963)
                     /gene="metH"
                     /locus_tag="RH222_003428"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV3798391.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14163..14987
                     /gene="iclR"
                     /locus_tag="RH222_003429"
     CDS             14163..14987
                     /gene="iclR"
                     /locus_tag="RH222_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV3798392.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3621 a         4013 c         3984 g         3617 t
ORIGIN      
        1 gttcagctcg caaacgaatt cccgtacact gaactctctg gctcactgag ggcaccatca
       61 tggcactccc ccgtattacc caaaaagaga tgactgaacg cgagcagcgt gaacttaaaa
      121 cactgctgga tcgggcgcgg attgcgcatg gtcgcgtact gaccaactcc gaaaccaaca
      181 gcattaagaa agagtacatc gataaattaa tggtcgagcg tgaggctgaa gcgaaaaaaa
      241 cccgccagtt gaagaaaaag caggcttata aaccggatcc tgaagcgtca tttagctggt
      301 cagcaaatac gtcgacgcgc ggcaggcgct gattagcgtc ccttcttttt acgccccggc
      361 gaggtaaaac gcttaccgtt ggacgccggg cgaccgcctt tttccgccgt tttttccatc
      421 ttaacgactg gacgtttgat gcccgctgtt ttcggtttag ccttcgcttt aggttttacc
      481 tcggaagagg aattttcaat gagtttaaac aggtcgatta actcatcgtc agttaaatcg
      541 cgccactcac ccagcggaat gccgcttaaa ctgacgttca taatgcgcgt acgttccagc
      601 tttttcactt catagccgaa atgctcgcac atgcgccgga tctgacggtt cagcccctgc
      661 accagggtaa tgcggaagac aaacggcgct tcttttttga ctttgcactt tttcgtcacc
      721 gtcccgagga tcggcacccc cgcccccata ccacgaataa attcatcggt aatcggttta
      781 tcgaccgtca ccagatactc tttctcatga tcgttacccg cacggaggat cttattcacc
      841 agatcgccgt gattggtgag gaaaatcagc ccctgggagt ctttatccag gcggccaatc
      901 gggaacacgc gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg
      961 gtggtgctga caataccgac gggcttgttc agggcgataa gtaccaaatc ttcggcttcc
     1021 cgaggctcaa tcaactgacc gtttactttc acaatgtcgc cgggtttcac ctgatcgcca
     1081 atggtggctc gcttgccatt aaggaacaca ttgccttgct cgatatagcg atccgcttcg
     1141 cggcgtgagc aaattccgct ttcgctgatg tatttattta aacggactga tgagtcgggc
     1201 agcataaatt ctcctgtaaa acgcggaata taccgtacct cttagcgggg aaaaacaggg
     1261 ttatgcggca acgtcgaaat taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc
     1321 cggaagtacg atgcaaattt gcctggcaaa ataaggataa tcaacatatt acgaatcgct
     1381 gcacacctgt gctgatgact actacgcaga gaaacaaatg tgcaagagaa agcgctattt
     1441 ctccccatgc aaagagtgaa aaatcgcttt gaaaggccct catgatgggg ttatatcaga
     1501 tcaagcggtg agaaagcgta tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa
     1561 cagtgacgat atccgtttga ttgtgaagat tgcccaactc tattacgaac aggatatgac
     1621 gcaggcgcaa atcgcgcgcg aactgcgtat ttaccgcacc accatcagcc gcttgcttaa
     1681 acgaggccgc gatcagggaa ttgtcaccat cgccatcaac tatgactaca acgaaaatct
     1741 ctggctggag cagcaactga agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg
     1801 aaatgatgag gatgaagaga ctcaactggc gatgatgggg ttacacggcg cgcaactgct
     1861 ggatcgcttg ctggagcctg gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc
     1921 actggttgaa aacttgccgc aggcggggca atcgcggcag ttaatctgcg taccaattat
     1981 tggcggaccg tccggtaaac tcgaaagccg ctatcatgta aacacattaa cctacagcgc
     2041 ggcagcgaag ctgaaagggg aatcgcatct cgcggatttt ccggctctgc tggataaccc
     2101 attaattcgt aatgggatca tgcagtctca gcactttaaa accatctctg cctactggga
     2161 taatctggat gtcgccctgg tgggaattgg ctcaccggcc attcgcgacg gcgctaactg
     2221 gcatgcgttt tatggtggtg aagagagtga cgacctgaat gcccgccagg ttgctggcga
     2281 tatttgctcg cgcttttttg atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa
     2341 aacgctctct atcgaaatga ataaattaaa gcaggcgcga tattccattg gcattgccat
     2401 gagtgaagaa aaatacagcg gaattattgg tgcactgcgt ggaaaatata ttaattgtct
     2461 ggtaacgaat agcagcacag ctgaactatt actgaaataa aaatcttccc gatgtttacc
     2521 gtaataaaaa ttacggcggg tccctattat ctaaaatcag gagtctgtta tgcaaacgtg
     2581 gttaaatttg caggataaaa tcattatcgt caccggcggc gcatccggta ttggtctggc
     2641 gattgtagag gaattattag cacaaggcgc aaacgtacag atggtagata ttcacggtgg
     2701 cgatggtcaa tatgaaggtc ataaaggcta tcagttttgg ccgaccgata tttccagcgc
     2761 caaagaggta aatcatacgg tagcggaaat tatccagcgt tttggtcgca tcgacggtct
     2821 ggtcaataac gccggggtca atttcccgcg tctgctggtc gatgagaaag cgcctgccgg
     2881 gcagtatgaa ctcaacgaag ctgcattcga aaaaatggtc aatatcaacc agaaaggcgt
     2941 ttttctgatg tcgcaggcgg tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt
     3001 gaatgtttcc tcagaaagtg gactggaagg ctcagaaggc caaagctgtt acgccgctac
     3061 caaagccgcg cttaatagct tcacgcgctc ctggtcgaaa gagctgggta aacacggtat
     3121 ccgtgtggtc ggtatcgcgc ccgggattct ggaaaaaacc gggctacgca cgccggaata
     3181 tgaagaggcg ctggcgtgga cgcgcaatat caccgtcgag cagctgcgtg aaggctatac
     3241 caaaaacgcc attcctattg ggcgcgccgg aagattagca gaagtcgctg attttgtttg
     3301 ttatctgttg tctaaacgcg ccagctacat caccggagta accactaaca ttgcgggcgg
     3361 caaaacgcgc ggctaaggag gctttatggt caacgccatt ttttgtgccc acggcaaatt
     3421 agcttgcgcc atgctggaat cggttcagat ggtttacggc aacgccaatg ttgaagccgt
     3481 tgaatttgtg cccggcgaga acgccggaga catcgtagca aagctggaaa agttagtaag
     3541 cattcacaat caagatgagt ggctgattgc cgtggattta cagtgcggca gcccgtggaa
     3601 tgcggcagcg atgcttgcga tgcgcaatcc tcgtctgcgg gtaatcagcg gcctttctct
     3661 gccgctggcg ctcgaactgg ttgataacca ggacagcatg aatgttgatg aactgtgtga
     3721 acacctgacg caaatcgcaa agcaaacctg cgttgtctgg aaacaactgg caacggcaga
     3781 ggaggatttc tgatgaacat tacgctcgcc cgcattgatg accgcctgat ccacggtcag
     3841 gtcactaccg tctggtcgaa agtggctaac gcccagcgca ttattatctg caatgacgaa
     3901 gtttataacg atgaagttcg ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag
     3961 gttaacgtgg tcaatattga aaaagccgtc gctgtttatc acaacccgca atatcaggac
     4021 gaaaccgttt tttatctttt cactcgccca caggatgcct tagcgatggt tcgccagggg
     4081 gtaaaaatag acaccctgaa tattggcggc atggcctggc ggcccggtaa aaaacagtta
     4141 accaaagcgg tttcattaga tgatgatgat attaacgcat ttcatgagtt aaataatctt
     4201 ggcgtcatac tggatttacg cgtcgtagcg tcagatccct caatcaatat tatcgacaaa
     4261 ataaacgaac agttaatcgc aaattaaata atagcgcctg tgtacatatg ctttaacagg
     4321 cagggatgac gacacttaaa aggtgaaaaa tcatggaaat aagtacccta caaatcattg
     4381 ctatatttct tttttcctgt attgccggaa tgggcagcgt gctggatgaa tttcagactc
     4441 atcgcccgtt aattgcctgt acggtgattg gtttaattct cggtgattta aaaaccggaa
     4501 taatactcgg tggtacgctg gaattgatag ctctcggctg gatgaacgtc ggcgcggcgc
     4561 aatctccgga ttctgcactc gccagcataa tctccgccat tctggttatc gttggtcagc
     4621 aaagcatcgc caccggaatc gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga
     4681 ctgtgtttgc ccgtaccatc accgtagtgt tccagcacgc ggcggataaa gcagcggaag
     4741 aagcacggtt tcgcactctc gatattctgc atgtctccgc gcttggcgtg caggcgctgc
     4801 gcgttgctat tccggcactg attgtctcac tgttcgtcag cgccgatatg gtgagcaata
     4861 tgctgagcgc gataccggaa ttcgtgacgc gcggtctgca aattgctggc ggttttatcg
     4921 tggtggtcgg ttacgccatg gtgctacgta tgatgggcgt gaaatatttg atgcctttct
     4981 ttttcctcgg tttcctcgca ggtggctacc tcgatctcag tctgctggcg ttcggtggcg
     5041 tcggcgtgat catggccctg gtctacatcc agctaaatcc acagtggcgt aaagctgaac
     5101 ctcaacccca ggccaccacc tccaccgccc ttgaccaact tgacgattaa cggagccatc
     5161 atggaacaga gaaaaattac acgcagcgat ctggtgagca tgtttctgcg ctccaacctg
     5221 caacaggcgt cctttaactt tgaacgtatt cacgggctgg gcttttgcta cgacatgatc
     5281 cccgccatca agcgacttta cccattaaaa gaggatcagg ttgcggcgct caggcgacac
     5341 ctggtgttct tcaataccac gccagccgta tgtggcccgg tcatcggcgt cactgccgcc
     5401 atggaagagg cgcgggccaa cggcgcggaa attgatgacg gtaccattaa cggcatcaaa
     5461 gtcggtctga tgggaccatt ggcaggggtt ggcgatccac tggtttgggg aacgctgcgc
     5521 ccgattaccg ctgcgctcgg cgcatctctg gcactttctg gcaacatcct cggcccgctt
     5581 ttgtttttct ttattttcaa tgcggtgcgt ctggcgatga agtggtatgg cctacagctc
     5641 ggctttcgca aaggggtgaa tatcgtcagc gatatgggcg ggaatgtgct gcaaaaactc
     5701 accgaaggcg cgtcgattct cggactgttt gtgatgggcg tgctggtcac caaatggacg
     5761 tcaatcaacg taccgttggt ggtttcacaa acgcatgccg ccgatggctc caccgtcacc
     5821 atgaccgtgc agaacattct cgaccaactt tgccctggtt tgctggcgct cggtctgacg
     5881 ctgcttatgg ttcgtctgct caacaaaaaa attaacccgg tatggctgat tttcgccctg
     5941 tttggcttag ggattatcgg taatgcgctg ggcttcctgt cctgattctt cgccccggca
     6001 cgactgccgg ggccatcgct caacatgagg tggtttatga aaacaacagc tctgcgtctt
     6061 tatggtaaac gtgatttacg cctggaaacc tttgaccttc ctgaaatgca ggaggatgaa
     6121 atcctcgcga cggtggtcac tgacagcctg tgcctctctt cctggaaaga ggccaatctg
     6181 ggtgaaaacc ataaaaaagt acccgacgat gtggcgacca accccatcat catcggccac
     6241 gagttttgcg gcgatattct ggccgtgggt aaaaagtggc agcacaaatt ccagccgggt
     6301 cagcgttatg tgattcaggc caacctgcaa ctccccgacc gcccggactg ccccggctac
     6361 tccttcccgt gggtgggcgg cgaagccacg catgtggtta ttcccaacga ggtcatggaa
     6421 caagattgcc tgctggcata cgacggcgaa acctattttg aaggctcact ggttgaaccg
     6481 ctttcctgcg tgattggcgc gttcaacgcc aactatcatc ttcaggaagg tagttataac
     6541 cacacgatgg ggattcgccc gcaagggcgc atgctgatcc tcggcggcac cggaccaatg
     6601 ggactgttgg cgattgatta tgcgctacat ggacccgtta acccgtcgct gctcgtcatt
     6661 accgataccg acaacgataa attgagttat gcgcgcaagc actatccatc agaaccgcaa
     6721 acactgattc attatctcaa tgccgccgat gtagcatttg atacgctaat ggcgctgagt
     6781 ggcggtcacg gcttcgatga tattttcgtc tttgtgccta atgaaggact ggtgactctc
     6841 gcctcttcct tgctggcgac agatggttgc ctgaatttct tcgccggacc gcaggataaa
     6901 catttcagcg cgccaattaa tttctacgat gtgcattatg catttaccca ctacgtgggc
     6961 acgtcaggcg gcaataccga cgacatgcgc gcggcggtca aactgattga agagaaaaaa
     7021 gtgcaggccg caaaagtggt aacacatatt cttgggctga atgccgcggg cgaaaccacg
     7081 cttgaattgc ctgccgtcgg cggcggcaaa aagctggtgt ataccgggaa atacctgccg
     7141 ctgacgtcac tcacgcagat tcaggatcaa gcactggcgg cgattctggc gcgtcatcag
     7201 gggatctggt ccggtgaggc ggagcaatat ctgctcactc atgcagaggc aatttcccat
     7261 gattaatcgc gctacttaac tctgcatgtc gctggctggt cgccgcggca acttcgcgac
     7321 ctgatgtgcg gctgattgct gaagcggccc aatgtgcacg agaaggataa aaaaacatca
     7381 gcgccttaag gggcgctcaa attggtggca aagtacacat tgtttatgcc ggatgcggaa
     7441 taaagcctta ttcggcctac ataatctcga aaattcaata tattgcagtt atctggtagg
     7501 cctggtaagc gtagcgcatc aggcaatgct gtgtttgtca tcagtctcag cgcccggtgc
     7561 aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcatgcctc
     7621 ccgtgatttc ttactaactg gagaagtaat ggaactgctt ttattgagta actcgacgct
     7681 gccgggtaaa gcctggctgg aacatgcgct gccgctgatt gctgaacagt tgcagggtcg
     7741 ccgttcagcg gtatttattc ctttcgctgg cgtaacgcag acctgggatg attacacagc
     7801 gaaaacggct gcggttctcg ctccgctggg tgtatctgta accggtattc atagcgttgt
     7861 cgatcccgtt gccgcgattg aaaatgctga gatcgtgatt gtcgggggcg ggaatacttt
     7921 ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca ccaattactg acgtggttaa
     7981 acgtggtgcg ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caaccattcg
     8041 taccaccaac gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc
     8101 gctgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaacccg
     8161 tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctacc
     8221 ggaaggtaac tggatcacag tgagtaaagg tcacgctacg ctgggtggcc cgaacaccac
     8281 ttatgtgttt aaggctggtg aagaagcggt gccgctagaa gctggtcacc gtttttaagt
     8341 aaattgccgg atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gcttgacgcg
     8401 tcttatcagg cctacaggat tcacaatatt aatactcgtc ccgtccttca tcttcatccg
     8461 gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcaggcgc tgcatatctc
     8521 ccagtaagcc taaatgtagc gaactggttt caatgctctg cacgttttgc tgatgcaggc
     8581 gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg aaaacgatgt ttgctgcgac
     8641 gcaaacgacg agcgctggtg acatcgccag agaagaacac cgacattgcc agctttaaat
     8701 tactgagcaa ttgctcatag agcgcatcca gttccttcaa cccatcaagc gaaaatgccc
     8761 gccgtgctgc cagcgattta tcggcaattt cgctgcccat gcgctcgacg atatcggaag
     8821 cctgttcaag gttgagcgac atttcaataa tctccgccca gcgacgcgac tcttcttctg
     8881 ccagttcctc ttttggcatc cgcgccagat acagcttaat ggcggtatag agaacgttga
     8941 tatcatctgc cagcttacgc agctctttct cctgccgtgg ctcgccgtgc atcactttat
     9001 tcagcccttc catcatctgt tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt
     9061 tcgccagagc aagcgtgggc gtatccagcg cgctgacatc cagatgttta ggccgtagct
     9121 gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa
     9181 caaatggcag catgaccagg caacgtacaa ggttgtagaa gacgtggaaa tagatcacca
     9241 gttccgcttt tggcaatgac agcttcccca ttgtctctgc cagcaaatgg acaaacggca
     9301 ggataatcag gctacccacc agcttaaaca gcagactacc cagcgcgaca cggcgggctg
     9361 cggcattggc ggcactgttg ttgagcatcg ccagcaggcc ggaaccgagg ttagcaccaa
     9421 tcaccagaca gagcgccacg gggaaggaga taatgcctgc ggcggtcaga gtcgcagtca
     9481 gcagtacagc agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat
     9541 ccagcagaat atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga
     9601 tcggcgttac ggcctgcaca atcaactcca gcgccagtaa aatcagccca agaccaatac
     9661 cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga
     9721 taaaaataag taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg
     9781 ccgtcccgac atctgccccc agcacaatga ccagagccgg tgcgagggct accagatcct
     9841 gagcgacaaa cgaggtcacc agcatggtgg tggcattact gctctgcacc agtgcggtaa
     9901 cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac gctccggcta aggacagtac
     9961 gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac aatatgagtc ccccagacca
    10021 gcagggcgac ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc
    10081 gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct
    10141 ttactgtgcc ttttgacatt cgtttttccg gctaagatta ttccagcctg tttccagtat
    10201 aagtgtttca tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga
    10261 aaagtgacag atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct
    10321 caacatcgga gacgctcata cctttacggc gggcatagtc ttcaacctga tcgcgctgaa
    10381 tttgcgccac cgcgtagtac ttgctgtccg ggtggctgaa ataccaaccc gaaaccgatg
    10441 caccgggcca catggcgaaa gattctgtga gtttcatgcc ggtatgtttt tccacttcca
    10501 gcagctccca gatggtggct ttttccgtat gttccgggca ggccgggtag cccggtgccg
    10561 gacggatgcc ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg
    10621 catagcccca gtagacttta cgcacacgct catggagata ctccgcaaag gcttcggcta
    10681 aacggtcggc aagcgctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg
    10741 catcagccag tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg
    10801 ctttaccaga aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aatcccgttt
    10861 tttcggtctg ttgacgcaga tggtggctga cgttgatcac atgggtgcgc gtttcgtcac
    10921 ggtagatttc aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg
    10981 gattcagcgt tttctcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc
    11041 gctgcgcctc aacgcccacc acttcatctt ccagaatgcg cggatacttc ccggccagcg
    11101 accaggtcat aaagaacggt gtccagtcga tgtaattacg cagagtttcg atgctggctt
    11161 caacttcctg cacgccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag
    11221 cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg tgtgcgcggt ttcttacgcc
    11281 cgtgctgaat acgtacggtt tcgtactcct tgcgggtacg agcgacaaaa tcatcacgct
    11341 gagtatcgga aagcagcgcc gccaccacac caacggtgcg cgatgcgttc tgtacataca
    11401 ccgtcgggcc gctgtagttc tgctcaattt tcaccgccgt gtgcgctttt gaggtcgtcg
    11461 cgccgccaat caatagcggg atggtgaagc cctgacgctc catctctttc gccacgttaa
    11521 ccatctcgtc cagcgacggc gtgataagcc ccgaaagtcc aatcagatca gcattcactt
    11581 ctttagcggt acggagaatt ttttccgcag gcaccataac gccgagatcg acaatttcgt
    11641 agttgttaca ttgcagcacc acaccaacga tatttttacc gatgtcgtgg acgtcgccct
    11701 tcacggtggc gatcaccatc ttgccgttgg ttttgccctg ctctttgctg gcttcaataa
    11761 acggttcgag atacgccacc gcctgtttca tgacgcgcgc cgatttgacc acctgtggca
    11821 ggaacatttt cccttcgcca aacagatcgc cgaccacgtt catgccgtcc atcaacgggc
    11881 cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc ttcttcggta tcctgctcga
    11941 taaactcggt aatgcctttg accagcgagt attccagacg tttattcact tcccacgagc
    12001 gccactccgc ctgctgggcg ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg
    12061 caagctccag taaacgctca gtgccatcat cgcgacgatt aagaatcaca tcttccaccg
    12121 cgtcgcgcag ttcagcgggc aggtcgtcgt aaatcgccag ctgcccggca ttgacgatcc
    12181 ccatatccat gccattgcga atagcgtagt agaggaacac tgcgtgaatg gcttcgcgca
    12241 ccggatcgtt gccacggaac gagaaagaaa cgttagatac gccgccggaa attagcgcgt
    12301 gcggcagttc gcgtttgatg tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt
    12361 gctcttcaat gccggtggca accgcgaaga tgttcgggtc gaagatgata tcttctggcg
    12421 ggaagccaac ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg
    12481 cgcgggtatc ggcctgtccc tgttcgtcaa aggccattac caccactgcc gcaccgtaac
    12541 gacgcaagag cttcgcgtga tggataaagg cgtcgacgcc ctctttcatc gagatagagt
    12601 taacaatgcc tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg
    12661 agtcgatcat aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac
    12721 gcaccatcgc cgcttcggca tcgagcatcc cttcatccat gttgatatcg ataatctgcg
    12781 caccgttttc cacctgctgg cgggccacat ccagcgcctc gctgtatttc tcttctttga
    12841 tcaggcgctt gaacttagcg gaaccggtga cgttagtccg ctcgccgacg ttcacaaaca
    12901 ggctgtcgtc gccaatgttc agcggctcca ggccggacaa acggcaggct acgggaattt
    12961 caggcagttt gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgtg
    13021 gcgtggtgcc acagcagccg ccgacgatat tgagaaaacc cgcttgcgcc cattcacgta
    13081 tctgttttgc catcgtgtcg gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg
    13141 cgttcgggtg cgcggtgacg tagcattccg caatccgtga cagctcctgc acgtactggc
    13201 gcagttcatc tggccccagc gcacagttca ggccaaaggt cagagcttcg gcgtggcgca
    13261 atgagttgta aaatgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga
    13321 tggtgccgga gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg
    13381 caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg
    13441 caccaccttc caccagcgct ttggtggact ctcgataagc cgccaccagc ccgtcaaaag
    13501 tgatattacg aaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggttg
    13561 ggccgagaac accggcaacg tagcgcggtt tctccggggt gcgcgcggtc cactcatcag
    13621 cacaggcgcg cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct
    13681 ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc
    13741 ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc cggtttactg agtaccagca
    13801 ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg
    13861 cttcgttcag tcgatagctc tggatcatgg tgcccatacc gccgtccagc accagaatac
    13921 gttcatttaa ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc
    13981 tcaacccaga tttaataaaa attcaacaaa ccatactggc ataaacgcat ctgtggtaaa
    14041 agcgaccacc acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac
    14101 tattgcatta gctaacaata aaaatgaaaa tgatttccac gatacagaaa aaggagactg
    14161 tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag
    14221 cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat
    14281 ccaatggcag tgtggcactc acggaactgg cgcaacaagc cgggttaccc aattccacga
    14341 cccaccgcct gctaaccacg atgcaacagc agggtttcgt gcgtcaggtt ggcgaactgg
    14401 gacactgggc aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta
    14461 atttgttagc aattgttcac cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg
    14521 tcaatatggc ggtgcttgat caaagcgatc acgaagcgat tattatcgac caggtacagt
    14581 gtacccacct gatgcgaatg tccgcgccta tcggcggtaa attgccgatg cacgcttccg
    14641 gtgcgggtaa agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc
    14701 gcaaagggtt acatgcctat acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag
    14761 atctcgccca aacgcgcaaa cggggttatt catttgacga tgaggaacat gcgctggggc
    14821 tacgttgcct tgcagcgtgt attttcgatg agcaccgcga accgtttgcc gcaatttcaa
    14881 tttccggacc gatttcacgt attaccgatg accgcgtgac cgagtttggc gcaatggtga
    14941 ttaaagcggc gaaggaagtg acgctggcgt acggtggaat gcgctgactt ttttctggcg
    15001 ggcagaggca atattctgcc cgtcatatct gagtggcaat agaataaggg tgtctgttaa
    15061 tcgcattgac gccaaaataa cttattgtca tacacttcgc acacttttat taactcagca
    15121 ttatttttaa acatcaaacc acttaattat taacaaaatc gcaagatctc attacgccga
    15181 aacttttacg gtttcattat gagattgagc gatttatgat atttctttac ttgta
//
LOCUS       DAPHJE010000106        15171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPHJE010000106 DAPHJE010000000
VERSION     DAPHJE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.107"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..141)
                     /locus_tag="RH222_003430"
     CDS             complement(<1..141)
                     /locus_tag="RH222_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV3798393.1"
                     /translation="MEFYENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIG"
     gene            318..1730
                     /locus_tag="RH222_003431"
     CDS             318..1730
                     /locus_tag="RH222_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV3798394.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVEAPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(1974..2894)
                     /locus_tag="RH222_003432"
     CDS             complement(1974..2894)
                     /locus_tag="RH222_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV3798395.1"
                     /translation="MTNFTTSTPHDALFKTFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRQPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQHRERIMTIAERIHNDGYIKGEQRILRLLLQNGADPEW
                     IQKITGLSAEQMQALRQPLPERERYSWLKS"
     gene            3373..4605
                     /gene="mdtM"
                     /locus_tag="RH222_003433"
     CDS             3373..4605
                     /gene="mdtM"
                     /locus_tag="RH222_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV3798396.1"
                     /translation="MPRFFTRHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLSLLIVGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNKLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELVEEQ"
     gene            4646..5926
                     /locus_tag="RH222_003434"
     CDS             4646..5926
                     /locus_tag="RH222_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV3798397.1"
                     /translation="MNKLIELRRAKRLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            6042..7193
                     /locus_tag="RH222_003435"
     CDS             6042..7193
                     /locus_tag="RH222_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV3798398.1"
                     /translation="MSLVTDLPAIFDQFSEARQTGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            7203..7970
                     /locus_tag="RH222_003436"
     CDS             7203..7970
                     /locus_tag="RH222_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798399.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQKF
                     ARMLESHLRMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            7967..8224
                     /locus_tag="RH222_003437"
     CDS             7967..8224
                     /locus_tag="RH222_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV3798400.1"
                     /translation="MKEFLFLFHSTVGFIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYGVSPR"
     gene            8289..9149
                     /locus_tag="RH222_003438"
     CDS             8289..9149
                     /locus_tag="RH222_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV3798401.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            9217..10395
                     /locus_tag="RH222_003439"
     CDS             9217..10395
                     /locus_tag="RH222_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3798402.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     PPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLSNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(10408..10962)
                     /gene="kptA"
                     /locus_tag="RH222_003440"
     CDS             complement(10408..10962)
                     /gene="kptA"
                     /locus_tag="RH222_003440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV3798403.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            11212..11895
                     /locus_tag="RH222_003441"
     CDS             11212..11895
                     /locus_tag="RH222_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798404.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTNSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11892..12353
                     /locus_tag="RH222_003442"
     CDS             11892..12353
                     /locus_tag="RH222_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV3798405.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            12366..13538
                     /gene="iadA"
                     /locus_tag="RH222_003443"
     CDS             12366..13538
                     /gene="iadA"
                     /locus_tag="RH222_003443"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV3798406.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYRLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13603..14514
                     /gene="hypT"
                     /locus_tag="RH222_003444"
                     /gene_synonym="qseD"
     CDS             13603..14514
                     /gene="hypT"
                     /locus_tag="RH222_003444"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV3798407.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(14507..14899)
                     /gene="iraD"
                     /locus_tag="RH222_003445"
     CDS             complement(14507..14899)
                     /gene="iraD"
                     /locus_tag="RH222_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV3798408.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14896..14979)
                     /gene="idlP"
                     /locus_tag="RH222_003446"
     CDS             complement(14896..14979)
                     /gene="idlP"
                     /locus_tag="RH222_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV3798409.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         3421 a         3880 c         3916 g         3954 t
ORIGIN      
        1 cccgatatcc ctcaaccgct catcactcat ctgctgtaac actcgtcggg tctgcatttg
       61 ccgccgccaa cgcctcaccg cctgccagag ttgcacaagt ctgataaacg gcgttttggc
      121 tttgttttcg taaaattcca tctgctgcct cctcatcttt cagaggcttc atcatccacc
      181 ttcaggcagt tcacaataca gatgcaaata ttagttttat ttaacataca gacaggttaa
      241 aacagcatct gcatccggtt ttttagcctc atctgtaccg gtttttctct ctgtatggtc
      301 caaataagga atacagcatg acgcgttatc aacatctggc aactctgctt gccgagcgga
      361 ttgagcaagg gctgtatcgt cacggggaga aattgccgtc ggtgcgcagc ttaagtcagg
      421 agcacggcgt cagtatcagc accgtgcagc aggcgtatca gacgctggag acgatgaagc
      481 tcatcactcc gcagccgcgt tcgggttatt ttgtcgcaca acgtaaagcc cagccgccag
      541 taccgccgat gacgcgtccg gtgcagcgcc cggtggaaat tacccagtgg gatcaggtgc
      601 tggatatgct ggaggcgcat agcgacagtt ccattgttcc gttaagcaaa agcacaccgg
      661 atgtcgaagc gcccagcctg aaaccgcttt ggcgggagct aagccgggtg gtgcagcata
      721 atctgcaaac cgttctcggt tatgacttgt tagccggtca gcgagtattg cgagagcaga
      781 ttgcccgcct gatgctcgac agcggctcgg tggtcaccgc cgatgacatc atcatcacca
      841 gcggctgcca taactcgatg tcgctggcgt taatggcagt gtgtaaaccg ggcgatattg
      901 tcgcggtcga atccccctgt tattacggtt cgatgcagat gctgcgcggc atgggcgtga
      961 aagtgattga aatcccaacc gatccagaaa ctggcatcag cgttgaagcg ctggaactgg
     1021 cgctggaaca gtggccgatt aaaggcatca ttctggtgcc aaactgtaat aatccgctgg
     1081 gatttattat gccggacgcg cgcaaacggg ccgttctctc tctcgctcag cgtcatgata
     1141 ttgtgatttt tgaagatgat gtctatggcg aactggcgac ggagtatccg cgcccgcgga
     1201 ccattcattc ctgggatatc gacgggcgag tgctgttgtg cagctcgttc agtaaaagta
     1261 ttgcaccagg cctgcgcgtg ggttgggtcg caccggggcg ttatcacgat aaactgatgc
     1321 atatgaaata cgccatcagc agctttaatg tgccgtccac gcaaatggcg gcggcaacgt
     1381 ttgtgctgga aggtcactat catcgccata tccggcggat gcggcagatc tatcagcgca
     1441 atttggcgct ttatacctgc tggatacggg aatattttcc ctgcgaaatc tgtattacgc
     1501 gcccgaaagg cggattttta ctgtggatag aattgcctga acaggtcgat atggtctgcg
     1561 tcgcgcggca gctgtgccgc atgaaaatcc aggtggcggc aggctcgatt ttctcagctt
     1621 ccggcaaata ccgtaattgt ctgcgcatca actgcgcttt gccgctcagc gaaacctatc
     1681 gcgaagcact gaagcaaatt ggcgaggccg tgtatcgggc aatggaataa attcgaaata
     1741 gcggcgaagc gcctgatttc ctctcatgca taacctgtga ctcgttttat ctttgctggg
     1801 tcatcgccac gttccagcct gaattaagca aagtacgctt tgttcatgcc ggatgccatg
     1861 tgaacgcctg accaggtcta caaaaatctt taaagttcaa tatatttaat gggttatgta
     1921 gacctgataa gcatagcgca tcaggctgct ttgcgtttgt catccgtctc tgattagctc
     1981 ttgagccatg aatagcgctc acgctcaggc aagggctgcc ttaatgcctg catttgctct
     2041 gccgaaagtc cggtaatctt ttgtatccat tcaggatccg ccccattctg caacaacaat
     2101 cgaagaatgc gctgctcccc ttttatatat ccatcattat gaattcgctc tgcaatcgtc
     2161 attattcgct ccctgtgttg tggcattcga cgggtaagtt cactgataaa ctcattaaaa
     2221 cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta
     2281 gcacactctg taaccagtaa tactaccagt tgatcgataa gccccatcag atcgcgctgg
     2341 cgaatatgct tttggatcaa ctccaacagg gcgactctgc gatgctgcac aatctcgtcg
     2401 tctggcacga cagtaacatc caccagcggg aacgcggcgt tataaagctt ccgtgcggta
     2461 gtcgggtcgg caaattcgtc cagccagcac agggaccagg ggtaaggact acggctaccg
     2521 tgataaaata gcatcgggat gaccaacggt agcggctggc gtttatcatg ctctatatgg
     2581 cgctgcatca ccgccatgga atagcgcatc aggcgaaagg ccatatgaat gtcctcgcgg
     2641 ctctgatgtt caatcaccac atagatatag ccatcgcctt cgcgggtctt taccgaccat
     2701 aaaatatcgg agtgtagcgc ccgcaatttt tcatcgacaa agctggcgga ttccagtttt
     2761 aagctatcga gatcgcacag ttcacgtaaa tctttaggta agtgaatctc cataaaatcc
     2821 cgcgcagtgt cagggtgcgt gagaaaggtt ttaaataatg catcatgcgg cgtgctggtc
     2881 gtgaagtttg tcatggcgat ccatcacctg ttgaaaaaga tggcctgact ttagcgacta
     2941 acaaaaccat tctcacccgg caatttccca ttccccgagg cgctttccag gctattttta
     3001 ttcttctgca aacgatacat ttttccggaa acaagctcgc gttttacatg tcctgtaccc
     3061 ggcagatatt cccctttcca ccgcgctctt ttgattaacc gcaaatttta attacactta
     3121 aggtgtatat tttctatgca acccatcaat tcaagaggtg taatgtgctg atgactattt
     3181 gtaatcgtta tacattctga cccgaagtca gaaagtattt ctctgtctgt gtgttcacag
     3241 gcagtgtggt tgattacatg aaattcagta catttgcagt ctcgttgccc ttctcacctg
     3301 cctttcgtca ttaccgacgg tattgaattt cgttttcccc gttggggttc tccggacaag
     3361 gagttgtttg ttatgccacg tttttttacc cgccatgccg ccacgctgtt tttcccgatg
     3421 gcgttgattt tgtatgactt tgctgcgtat ctgtcgacgg atctgatcca gcctgggatc
     3481 attaatgtgg tacgtgattt taatgccgat gtcagtctgg cccctgctgc cgtcagtctc
     3541 tatcttgctg gcggtatggc gttacagtgg ctgctggggc cgctttccga cagaattggc
     3601 cgcaggccgg tgctgattac cggggcgcta atttttaccc ttgcctgcgc cgcgacaatg
     3661 ttcacaacgt ctatgacaca gtttcttatc gcgcgtgcaa ttcagggcac cagtatctgt
     3721 tttattgcca ccgttggtta tgtcacggtg caggaggcgt tcggacagac aaaagggatc
     3781 aagttgatgg cgattatcac ctccatcgta ctgattgcgc cgattatcgg cccgctttcc
     3841 ggcgcagctc tgatgcactt tatgcactgg aaagtccttt ttgccatcat tgcggttatg
     3901 ggttttatct catttgttgg cttactgttg gcgatgccag agacggtgaa gcgcggcgcg
     3961 gttccgttta gcgccaaaag cgtcttgcgc gattttcgta atgtcttttg caatcggctg
     4021 ttcctctttg gcgcagcaac catctcttta agctatatcc cgatgatgag ctgggtggct
     4081 gtctcgccgg tgatccttat cgatgcaggc agcttaacaa cttcgcagtt cgcctggaca
     4141 caggttccgg tgttcggcgc ggtgattgtt gcgaatgcca tcgtggcgcg ttttgttaaa
     4201 gatccgaccg aaccgcggtt tatctggcgt gccgtaccca ttcaactggt cggcctctcg
     4261 ctgttgattg tcggcaatct gctgtcgccg cacgtctggc tgtggtcggt gctgggcacc
     4321 agtctgtatg ctttcgggat tggtttgatt ttcccgacct tattccgctt tacgctgttt
     4381 tccaataagt taccgaaagg gaccgtctcc gcatcgctaa atatggtgat cctgatggtg
     4441 atgtcggtct cggtcgaaat cggccgctgg ctatggttta acggcggtcg cttgccgttt
     4501 catctgttag ccgttgtggc gggcgttatc gtcgttttca ccctggcggg attgctcaat
     4561 cgcgtgcgcc agcatcaggc agccgagcta gtggaggagc agtgattttt gcgcgatcct
     4621 gccgtcaggc tctattctta acgttatgaa taaactcatc gaactcagac gcgccaaaag
     4681 gttggcgctc tctttactgc ttatcgccgc tgctaccttt gtcgttacgc tgtttttgcc
     4741 gcccaatttt tgggtgagcg gcgtgaaggc gattgctgaa gcggcgatgg tcggcgcgct
     4801 ggcggactgg tttgcggtgg tggcgctgtt tcgccgcgtg ccgattccga tcatttctcg
     4861 ccatacggcg attatcccgc gtaataaaga ccggattggc gaaaatctcg gccagttcgt
     4921 gcaggaaaaa tttcttgata ctcaatccct ggtggcattg attcgacgcc acgaaccggc
     4981 gttgctgatt ggcaactggt ttagccagcc agaaaacgcc cgccgcgttg gtcagcatct
     5041 gttgcagatc atgagcggtt ttctcgaact taccgatgat gcgcgtattc agcgcctgct
     5101 taagcgcgcg gtccatcggg cgattgataa ggtcgatctt tccggcacca gtgcgttgat
     5161 gctggagagt atgaccaaaa acgatcgtca tcaggtgctg ctggatacgc tgatcgcaca
     5221 gttgatcgcc cttctccagc gcgataaatc gcgcaagttt atcgcccagc aaattgttcg
     5281 ctggctggag agcgagcatc cactgaaagc caaaattctc cccaccgaat ggttgggcga
     5341 acatagcgcg gagttggttt ctgacgcggt gaattctttg cttgatgata ttagtcgcga
     5401 tcgtgcgcat cagatccgcc atgcgtttga tcgcgccacc ttcgccctga tcgacaagct
     5461 gaaaaacgat ccggaaatgg cagcgcgagc cgatgccgta aaaagctatc tgaaagaaga
     5521 tgaagctttt aatcgctatc tcagtgaatt gtggggggat ttacgggaat ggctgaaagt
     5581 ggatatcaac agtgaagatt ctcgtgtgaa agaacgcatc gcacgagcgg gtcaatggtt
     5641 tggcgaaacg ttaattgccg atgatgcctt gcgggcgtcg ttaaatggtc atcttgaaca
     5701 agccgcgcac cgcgtcgcgc ctgagttttc cgcattcctg acgcgccaca tcagcgatac
     5761 ggtaaaaagc tgggatgcgc gggatatgtc gcggcaaata gagttaaata tcggcaaaga
     5821 tctgcagttt attcgtgtca acggtacgct ggttggcggt tgtattgggc taattttata
     5881 tttgctgtcg cagctcccgg ccttgttccc cctcggcaat ttttagaaat ccatgaaaaa
     5941 ctaagtggct aaccgcgaga gagatcaaat aaaccatact aaaagaatgg taataatgtg
     6001 gcctcttttc attatctccc gtggtacggg gaaggaaaat catgtcactt gtcaccgatc
     6061 tacccgccat tttcgatcag ttctctgaag ctcgccagac aggctttctc accgtcatgg
     6121 atctcaagga gcgcggcatt ccgctggttg gcacttactg cacctttatg ccgcaagaga
     6181 tcccgatggc agccggtgcg gttgtggttt cgctctgttc cacctctgat gaaaccattg
     6241 aagaagcgga gaaagatctg ccgcgcaacc tctgcccgct gattaaaagc agctacggct
     6301 tcggcaaaac cgataaatgc ccctacttct acttttcgga tctggtggtc ggtgaaacca
     6361 cctgcgacgg caaaaagaaa atgtatgaat acatggcgga gtttaagcct gttcatgtga
     6421 tgcaattgcc caacagcgtt aaggacgatg cctcgcgtgc gttatggaaa gccgagatgc
     6481 tgcgcttgca aaaaacggta gaagaacgtt ttgggcacga gattagcgaa gatgctctgc
     6541 gcgatgccat tgcgctgaaa aaccgcgaac gtcgcgcact ggctaatttt tatcatcttg
     6601 ggcagttaaa tcctccggcg cttagcggca gcgacattct gaaagtggtt tacggcgcaa
     6661 ccttccggtt cgataaagag gcgttgatca atgaactgga tgcaatgacc gcccgcgttc
     6721 gtcagcagtg ggaagaaggc cagcgactgg acccgcgtcc gcgcatttta atcaccggct
     6781 gcccgattgg cggcgcagca gaaaaagtgg tgcgcgcgat tgaagagaat ggcggctggg
     6841 ttgtcggtta tgaaaactgc accggggcga aagcgaccga gcaatgcgtg gcagaaacgg
     6901 gcgatgtcta cgacgcgctg gcggataaat atctggcgat tggctgctcc tgtgtttcgc
     6961 cgaacgatca gcgcctgaaa atgctcagcc agatggtgga ggaatatcag gtcgatggcg
     7021 tagttgatgt gattttgcag gcgtgccata cctacgcggt ggaatcgctg gcgattaaac
     7081 gtcatgtgcg ccagcagcac aacattcctt atatcgctat tgaaacagac tactccacct
     7141 cggatgtcgg gcagctcagt acccgtgtcg cggcctttat tgagatgctg taaggagtgg
     7201 cagtggcata ttcgattggc attgattccg gctcaaccgc caccaaaggg atcttactgg
     7261 cagacggcgt gattacgcgc cgtttcctcg ttccaacccc ctttcgcccg gcaacagcaa
     7321 ttactgaagc ctgggaaact ctgcgcgaag ggttagagac aacgccgttt ctgacgctca
     7381 ccggctacgg gcggcaactg gtggattttg ccgataaaca ggtaacggaa atctcctgtc
     7441 acgggctggg cgcacggttt cttgcgccag caacgcgcgc ggtaatcgac atcggtggtc
     7501 aggacagcaa agtgattcag cttgatgatg acggtaacct gtgcgatttc ctgatgaatg
     7561 acaaatgcgc ggcgggcacc gggcgtttcc tggaggtgat ctcgcgcacg cttggcacca
     7621 gcgtcgagca actcgacagc attaccgaaa atgtcacgcc gcacgccatc acgagtatgt
     7681 gcacagtgtt tgctgaatca gaagcgatca gcctgcgctc agcgggcgtc gcgccagaag
     7741 cgattctcgc cggagtgatt aacgcgatgg cgcggaggag tgccaatttc attgctcgtc
     7801 tctcctgtga agcgccgatt ctgtttactg gtggcgttag tcattgccag aagtttgccc
     7861 ggatgctgga atctcacctg cgaatgccgg taaataccca tcctgatgcg caatttgctg
     7921 gcgcaattgg cgcggcggta attggtcaac gagtgaggac acgccgatga aagagttttt
     7981 atttttattt cactccacgg tcggcttcat acaaacccgc aaagcgttgc aggcagcggg
     8041 catgactttt cgcgtcagcg atattccccg tgatttacgc ggcggctgcg ggttatgcat
     8101 ttggctgacc tgtccccccg gcgaggaaat acaatgggtg atccctgggc tgactgagtc
     8161 aatttattgc cagcaggatg gtgtctggcg ctgcattgca cattacgggg tttctccgcg
     8221 ttaaggattc attaaggtcg ctacgtatac ttctcgatat cttattgatt tgccttaaag
     8281 ggattaccgt gacaaaaagt atttcgttaa gtaaacgcat ctccgttatt gtgattcttt
     8341 ttgccattgt ggcagtgtgt actttcttcg tgcaatcttg cgcccgtaaa agcaatcatg
     8401 cagcatcatt tcagaattac cacgccacca ttgacggcaa agaaattgca ggcataacga
     8461 ataatatctc ttcgctgacc tggtcggcac aaagcaatac tctttttagc actatcaaca
     8521 aacctgccgc cattgttgaa atgaccacca atggtgattt tatccgcaca attcctctgg
     8581 attttgttaa ggatctcgag acgatcgaat acattggcga taatcagttt gtcattagcg
     8641 atgagcgcga ttacgccatt tatgtgattt cactgacccc aaactcggaa gtaaaaatcc
     8701 tcaaaaaaat aaaaatcccg ctgcaagact ccccaaccaa ttgcggtttt gaagggttgg
     8761 cttattctcg ccaggatcac actttctggt tttttaaaga gaaaaatccg attgaggtct
     8821 acaaagttaa cgggctgtta agtagcaatg aattacacat cagcaaagac gaggcactac
     8881 aacggcaatt tactttagat gatgtgtccg gcgcagaatt taaccagcaa aaaaatacgc
     8941 ttctggtgct gtctcatgaa tctcgcgcac tccaggaagt aaccctggtt ggcgaggtta
     9001 tcggtgaaat gtcactaacc aaaggcagtc gaggtttgtc gcataacatc aaacaggccg
     9061 aaggggttgc gatggatgcc tctggtaata tttatatcgt tagcgaacca aatcgttttt
     9121 atcgctttac ccctcaatca tcgcattaac aggctggcat gcttcacctt tttgttatta
     9181 acttgttaac aacaaaaatg acaaacaagg ttaaacatgc cttcgtccac gcatcccgta
     9241 gaacgctttt ctttcagcac cgcgcttttc gggatgctgg ttctgacctt aggtatgggt
     9301 ttaggccgct tcctttatac gcctatgttg cccgtcatga tggcggaagg atcgttttca
     9361 tttagccagc tctcgtggat tgccagcggc aactatgcgg ggtatctggc tggcagtctg
     9421 ctattttctt ttggcgcatt tcaccagcca tcgcgcctgc gcccatttct gttggcttct
     9481 gccctggcga gcgggttgtt gatcctcgca atggcatggt tgccgccatt tattctggtg
     9541 ttattgattc gcgtcctggc gggtgtcgcc agcgccggta tgctgatttt tggttcgacg
     9601 ctgattatgc agcacacgcg ccatcctttt gtgctggcag ctttgttttc tggcgttggc
     9661 attggcatcg cactgggcaa tgaatatgtt ctggcaggcc tgcattttga cctctcctcg
     9721 caaacgttat ggcaaggcgc cggggcgctt tctggcatga tgctgattgc acttacgctt
     9781 ttaatgccct cgaaaaaaca cgccatcaca ccaatgccat tggcaaaaac ggagcaacag
     9841 ataatgagct ggtggttact ggctattctg tacggcctgg cgggatttgg ttatatcatc
     9901 gtcgcgactt acctgccgct catggcgaaa gatgcaggct caccattgtt aaccgcccat
     9961 ctctggacgt tagtcggctt atcaattgtg cctggttgct ttggctggct atgggcagca
    10021 aaacgttggg gggctttgcc ctgcctgacg gcgaatttgc tggtgcaggc tatctgtgtg
    10081 ctgcttactc tcgccagcga ctcgcctctc ttgcttatca tcagcagtct tggttttggc
    10141 ggtaccttta tgggcacgac atctctggtg atgactatcg cccgccaact gagcgtaccg
    10201 ggaaatctca atcttttagg ctttgtgacg ctcatttacg gcattgggca aatcctcggc
    10261 ccggcgctga ccagtatgct cagcaatgga acatcggcgc ttgccagcgc cactctctgc
    10321 ggcgcggcgg cgttgtttat tgctgcgctt atcagcacag tgcaattatt taagctacaa
    10381 gtggtcactt catgagggaa gtttccctta ccactcaaga aactcaaccg caaccgttga
    10441 tgtcagccag accccgtttt ccgcttgcca gaaagggagg cctcgtttcg ccatttcctg
    10501 tgctttgacg gttaaaataa ccggcgatcc gtgtcttgct cccactttgc gtgccgttgc
    10561 ttcatcagca gagagatgta cgtagtgacg ctcacctgca atcagcccct gtttttttat
    10621 ctcgtcaagg aaacggcttg ccgtaccgtg gtagagaaac tgtggcggtg ttttttcggc
    10681 aaaggaaata gcgacctgcg aagtcgaatg cccctgaacc gcgcggatgc acctaccatc
    10741 actggaataa ctaaaacgct ttttatcgct ggtggcaacc acagtatcca gcaatgcacg
    10801 ggttaaccgc ttgcccgctt tttgggcaca aagtatgagc ttatcgatat ccgcccatcc
    10861 ttcacggtcc agtacgatac caatcgcctc cggtttgtgt cggaggacaa aacttaaaaa
    10921 cttgctcgta tccgccagct ctttttcgtt gtactttgcc atctcgtttt cgtccctgga
    10981 ttttctgttt ttatttactg atgtcattgt cacttggttt atgaattttc tgcaactcaa
    11041 tcctgcgcag agccttttcc acacagcgta atttttccgc tatgcaaaaa acgcatagcc
    11101 agaaacatac ttgcattttc cccataccca catggctgat aaggtttttc agttccagga
    11161 attttcctgg ctcaagcaac acacaaacac aaaaaaacaa tggggattgt tatgacacaa
    11221 caaggggatg ctgtagccgg ggagctggcg acagaaaaag tgggcattaa agggtatctg
    11281 gccttttttc tcaccataat ctttttttcc ggtgtttttt ccggaacaaa cagctggtgg
    11341 cgcgtttttg atttttccgt tctgaacgga tcgtttgggc aactgcctgg tgcgaacgga
    11401 gccaccacct catttcgcgg cgcgggtggc gcgggggcta aagatggctt tctctttgcg
    11461 ctggagctgg caccgtcagt cattctctct ctggggatta tttcaattac cgatggtctt
    11521 ggtggattac gcgccgcgca gcagttaatg acacctgtgc taaaaccgtt gttgggcatt
    11581 cctgggatct gctcgctggc gctgatcgct aacctgcaaa acaccgatgc cgccgccggt
    11641 atgaccaaag agctggcgca ggaaggtgaa attaccgaac gcgataaagt gattttcgcc
    11701 gcatatcaaa ccagcggtag cgccatcatc accaactact tctcttccgg cgtcgcggtg
    11761 tttgcctttc tgggcacgtc cgtaatcgta cctttagccg tcatcctggt gtttaaattt
    11821 gtcggagcca acattttgcg cgtctggctt aacttcgaag aacgtcgcaa cccaacgcaa
    11881 ggagcgcaag catgacaact caggtccgca aaaacgtcat ggatatgttt atcgatggcg
    11941 cgcgccgtgg ctttactatc gccacgacca acctgctgcc aaacgtggtg atggcgtttg
    12001 tcatcattca ggcgctgaaa attaccggcc tgctcgactg ggtggggcat atctgtgaac
    12061 ccgtaatggc gctgtgggga ttacccggtg aagcggcaac cgtcctttta gccgcattaa
    12121 tgagtatggg tggcgcagtc ggtgttgccg ccagtctggc aaccgctggc gcattaaccg
    12181 gtcacgatgt caccgtttta ctgcccgcga tgtatttaat gggcaacccg gtgcaaaatg
    12241 tcggtcgctg cctggggaca gccgaagtga atgccaaata ttacccgcat atcattacgg
    12301 tctgcgtgat taacgcattg ctgtcgatct gggttatgca gcttattgtt taacaaggag
    12361 ttaccatgat tgattatacc gcagccggtt ttaccctgct gcagggagcg catttgtatg
    12421 cgccggaaga tcggggaatt tgcgatgtcc tcgtcgctaa cggcaaaatt atcgccgttg
    12481 ccagcaatat cccttctgac attgtaccga actgcacggt tgtcgatctc agtgggcaga
    12541 tcctctgccc aggttttatt gatcaacacg tccatttgat tggcggtggc ggcgaagcag
    12601 gtcccacgac gcgcacgccg gaagtggcgc taagtcgcct gacggaagcg ggcgtcacgt
    12661 cagtggttgg tctgctgggc accgactcta tctctcgcca cccggaatcc ctgctcgcca
    12721 agacccgtgc gctcaatgaa gaaggcatca gcgcctggat gctgaccggc gcttatcatg
    12781 tcccttcccg caccattacg ggttccgtgg aaaaagacgt ggcgattatc gatcgtgtga
    12841 ttggcgtgaa atgcgccatc tctgatcacc gttctgccgc accggacgtt tatcgcctgg
    12901 ccaatatggc ggcagaatcc cgcgttggcg gtttgctcgg cggtaaacct ggcgtcaccg
    12961 tgttccacat gggcgacagt aaaaaggcgt tacagcctat ttatgacctg ctggaaaact
    13021 gcgatgtgcc gatcagcaag ctgctgccga cccacgttaa ccgcaacgta ccgttgtttg
    13081 agcaggcgct ggagttcgcg cgcaaaggcg gcaccatcga tatcaccagc agcattgacg
    13141 aaccggtcgc ccctgccgaa ggtattgccc gcgccgttca ggcgggtatt ccgctggcac
    13201 gcgtcaccct cagctccgac ggcaacggta gccagccgtt cttcgatgac gaagggaatt
    13261 taacccatat cggtgttgcc ggttttgaaa cgttgctgga aaccgtgcag gtgctggtca
    13321 aagactatga tttcagtatc agcgatgccc tgcgcccgct caccagtagc gtagccggtt
    13381 tccttaacct gaccgggaaa ggcgaaattc tgccaggcaa tgatgctgac ttgctggtca
    13441 tgacgccaga actgcgcatt gagcaggtat acgctcgcgg caaactgatg gtcaaagacg
    13501 gcaaagcctg cgtgaaagga acgtttgaaa cggcttaaac ggttgtcagc cgcccgaatg
    13561 gacgtagaat gcccgcaagg gcggctgaca gagtaaaacg taatggatga ctgtggtgcg
    13621 attttgcata atattgaaac caaatggctt tatgattttc tgaccctgga aaaatgccgc
    13681 aatttttccc aggcggcagt cagtcgcaac gtctcgcaac cggcattcag ccgccgcatc
    13741 cgtgcgctgg aacaggcgat tggtgttgaa ttgtttaacc gccaggtaac gccgctgcaa
    13801 ctctcagaac aagggaaaat cttccattcg cagatccgcc atctgttgca acagttagag
    13861 agcaatctgg cagagctgcg tggcggcagc gattacgcgc aacgaaaaat caagatagcc
    13921 gctgcacact ctctttccct cgggctgtta ccgtccatta tcagccagat gccgccgctc
    13981 tttacctggg caattgaagc tattgatgtc gatgaagcgg tcgataaact gcgtgaaggg
    14041 caaagtgact gtattttttc ctttcacgac gaagatttgc tggaagcgcc gtttgaccac
    14101 attcgcttat ttgaatctca attgttccct gtctgcgcca gtgacgaaca cggagaagca
    14161 ctttttaacc tcgcgcagcc acactttccg ttactgaatt acagccgcaa ctcctacatg
    14221 gggcgattga ttaatcgcac cctgacgcgc cacagtgagt taagtttcag cacctttttt
    14281 gtctcttcga tgagcgagct tttaaagcag gttgccctcg acggctgtgg gattgcctgg
    14341 ctgccggagt acgccatcca acaagaaatt cgcagcggga agctcgttgt actgaatcgg
    14401 gacgaactgg tgatcccgat tcaggcctat gcataccgga tgaatacccg catgaatccc
    14461 gttgccgaac gcttctggcg tgaactgcgc gagctggaga ttgtgcttag ctgacattct
    14521 ccagcgtcgc actgcgccaa ccgccattat cccagaccag atcaaacgtt agcggttcat
    14581 cgtcgtggta cagcattatc tcaaggctga tcgccagagt gtggcagtac gcttctttca
    14641 aattaacctg caacgccgca atccgtggct catgaaataa cagtgcagaa cgcagctgac
    14701 ggcaatacca gctctggaga tgtttgtcgc tcgggttatg cacttgccac tcgggcatgc
    14761 ctgaaacaag taatgacggt aatgccgaat gaggagaatt aaaaagagtg aggagatcgc
    14821 tgcataccga tttacgcagc gatgacgttt tattgcctga aatttcaggt aaaacagcct
    14881 gaagtgattg tcgcatcatt ttgcgcactc ctgacgttta gcaacatatg cggcttgccc
    14941 tgaacaccgg gcaccactgt attgatgctc attttccata gccattccct tttgttcatt
    15001 acatccatgt cagtctgaga caggcctgaa tcctgtcagc aaagtataaa accttacttg
    15061 cctatgaata tctactgact tttcaggaaa aaagtcatga tttgataaaa atcatcaaac
    15121 tcaaaaaaaa accgctagta tattctgaat attccgaata gttacgtaga t
//
LOCUS       DAPHJE010000107        15014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPHJE010000107 DAPHJE010000000
VERSION     DAPHJE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.108"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(240..944)
                     /locus_tag="RH222_003447"
     CDS             complement(240..944)
                     /locus_tag="RH222_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3798410.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWTVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1081..1533)
                     /gene="moaE"
                     /locus_tag="RH222_003448"
     CDS             complement(1081..1533)
                     /gene="moaE"
                     /locus_tag="RH222_003448"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV3798411.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1535..1780)
                     /gene="moaD"
                     /locus_tag="RH222_003449"
     CDS             complement(1535..1780)
                     /gene="moaD"
                     /locus_tag="RH222_003449"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV3798412.1"
                     /translation="MIKVLFFAQVHELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1773..2258)
                     /gene="moaC"
                     /locus_tag="RH222_003450"
     CDS             complement(1773..2258)
                     /gene="moaC"
                     /locus_tag="RH222_003450"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV3798413.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2261..2773)
                     /gene="moaB"
                     /locus_tag="RH222_003451"
     CDS             complement(2261..2773)
                     /gene="moaB"
                     /locus_tag="RH222_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV3798414.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2795..3784)
                     /gene="moaA"
                     /locus_tag="RH222_003452"
     CDS             complement(2795..3784)
                     /gene="moaA"
                     /locus_tag="RH222_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV3798415.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3772..3916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4181..5089
                     /gene="yvcK"
                     /locus_tag="RH222_003453"
     CDS             4181..5089
                     /gene="yvcK"
                     /locus_tag="RH222_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV3798416.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5281..7302)
                     /gene="uvrB"
                     /locus_tag="RH222_003454"
     CDS             complement(5281..7302)
                     /gene="uvrB"
                     /locus_tag="RH222_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV3798417.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7881..8558)
                     /gene="bioD"
                     /locus_tag="RH222_003455"
     CDS             complement(7881..8558)
                     /gene="bioD"
                     /locus_tag="RH222_003455"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV3798418.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8551..9306)
                     /gene="bioC"
                     /locus_tag="RH222_003456"
     CDS             complement(8551..9306)
                     /gene="bioC"
                     /locus_tag="RH222_003456"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV3798419.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9293..10447)
                     /gene="bioF"
                     /locus_tag="RH222_003457"
     CDS             complement(9293..10447)
                     /gene="bioF"
                     /locus_tag="RH222_003457"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV3798420.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10444..11484)
                     /gene="bioB"
                     /locus_tag="RH222_003458"
     CDS             complement(10444..11484)
                     /gene="bioB"
                     /locus_tag="RH222_003458"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV3798421.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11571..12860
                     /gene="bioA"
                     /locus_tag="RH222_003459"
     CDS             11571..12860
                     /gene="bioA"
                     /locus_tag="RH222_003459"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV3798422.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGKR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12919..13395
                     /locus_tag="RH222_003460"
     CDS             12919..13395
                     /locus_tag="RH222_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV3798423.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(13825..14142)
                     /locus_tag="RH222_003461"
     CDS             complement(13825..14142)
                     /locus_tag="RH222_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798424.1"
                     /translation="MAFPSPASDYVEQRLSVNSICNVGPNTLVFERSGGYVVLDISLK
                     PRQGSQVLIQHGGGTELATLRGRSLITEDGEAIEGEALDDITVAGVVTHTIIDVRSDS
                     VAV"
     gene            complement(14447..>15014)
                     /locus_tag="RH222_003462"
     CDS             complement(14447..>15014)
                     /locus_tag="RH222_003462"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798425.1"
                     /translation="YLYAKNCIFDLSVTRNAFNSLGVNCIMQNCTAQYGMQDAFNYHA
                     DQNGLGIKPWFIEIGCTGRWSGFDNLPNNNGSTAHDGTVGIRLNGEYYGTFGRVVHDV
                     HDGTVTANFGCYSHDSSRADFIGGACFTAGQGSDLTGKAKLYLYGCSHGGPNASITKD
                     GLSEVWIFDTPIGANAQYTIASPYTFIQ"
BASE COUNT         3558 a         4030 c         3768 g         3658 t
ORIGIN      
        1 tctaatttat tgctgttttg tgagtatgat tccattcttt tagttgtcct tagaaaaaat
       61 attaatattc accatcaata aatttaaaac cacgcataca aaatactttt gtatttatgg
      121 atacggaaaa cgcttccgct cgccataagg cgtattttat acctggtact tgttaagtgt
      181 cgtttttagt aagaaaaaaa atcccgacac atggccggga tattgatggt gaaaaagaat
      241 taacggcggt tgccaaaaat tcgcagcaac atcaggaaca ggttgatgaa gtccagatac
      301 aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg ggtatcaatc
      361 tgctcaccca tatttttcaa tttctgagtg tcatacgccg tcaatccgac aaagacaatc
      421 acgccgatgt aggtaactgt ccacatcaat gcttcgcttt tcagccagaa gttgaccagc
      481 gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc acttaaatcg
      541 cgcttcgtgg tgtaaccgta caggctcatt gcgccgaaca tcccggcagt aacgacgaaa
      601 gtactggcga tagaagcagc ggtatagaca atgaatatac tggaaagcgt aagacccgtc
      661 agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt ttgaatcatc
      721 gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa aaagacacgg
      781 ttagtaaaca acagctccat cacggccgcg gaattagccg cataccaggc aacaaatgcg
      841 gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt ttgcaagcca
      901 gcccggggtt gtacgattga atcagaacgt gggaatctgt ccatgacgat ctcctgaaga
      961 tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac gcagcgtaaa
     1021 cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc taacaaaaaa
     1081 ctaccagcgt tttgccgcct gctgatcgct ctcccgagct tcaacccagc ggtcgccttc
     1141 cggcgtggct tcgcgcttcc agaacggtgc gcgggttttg agataatcca taataaactg
     1201 cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa cgatttcatc
     1261 gcccggccat aattccccga tgcggtgaat cacagtgacg cgccccagcg gccagcggtt
     1321 acgcgcttca tcaacaattt ctgccagtgc tttttcagtc atccccggat agtgttcgag
     1381 ggttaatgct ttgacgctgt cgcccaggtt atggttgcgc accttaccag taaaggtgac
     1441 taccgcaccg tcttcgtcac gctccgccag ccacgggtac tcttctccta cgctgaacgg
     1501 ctgcggacca acaacaattt tggtttctgc catcttaacc tccggttacc ggcgggaaga
     1561 aagctacttc gtcgccgtca gtcagcggat ggtcaaaact caccagcgtc tggttgacgg
     1621 cagccagtaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc gcagccatgt
     1681 gctggcgtaa cgcttcaaca gttgggaaat ccgcagccac ttcggttgca tctgttccca
     1741 ccaactcgtg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac cttaaagtca
     1801 cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caatcaccat atctttttgc
     1861 accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt taatgcttcc
     1921 atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgcac ccgattgtgc
     1981 tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcggatg acagagcggg
     2041 atcagatccc aggtgcgttt tgccgcctga ataccggcaa tacgcgcagt ggcaaatacg
     2101 tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg catggtgaca
     2161 aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc caccatgtgc
     2221 gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc aaatgtggat
     2281 ggaaattaca cggacgcgta cgggcatcca gctgcggcgc gatgatattt tcccatgcgg
     2341 tacggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgttg gcgacgcccg
     2401 ctaccgcacg agattgcaac gtggaagtgc caatctcttc aaacgacaac atacggaaca
     2461 cttcaccaaa accttcaatt tcacggtcga acaacggcag caatgcttcg ggagcctgat
     2521 caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg tcgctggcga
     2581 tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg gctttatcga
     2641 caacgtgatg gcccgcttct tgcgccgaat cgcgcagata gtgaccggag gtatcgtctt
     2701 cttcaccgcg acgattagaa accgtaagaa tagcaatacg ggtcgggata aattcagtgc
     2761 ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac gataagtttt
     2821 gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc cgcagcgccg
     2881 ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga tcgcgcaggt
     2941 taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa acgcgcaggc
     3001 ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc tctccggcgt
     3061 aatctggatg gcaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat tggtggatcc
     3121 agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc ttacggaaga
     3181 gctcgctgcc ctcgcccgtt tccatcagtt cgatgaaacg cagctggata gggcgatgct
     3241 ggatccagtt cagaaaggtg tcgagctggt gatgattaac atcacgcatc agcacggtat
     3301 tgactttgac cttctcaaaa ccggcctcaa atgcagcatc aatccccgcc atgacctggt
     3361 tgaatttatc ctgcccggta atagcgtgaa actggcgggc gtccagactg tcgacactga
     3421 cgttaatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc agacggtaac
     3481 cattggtggt gaccgcaatc tggcggatag cgtcgttttc ccgcacagcg gcgatgatat
     3541 cggtaaagtc gcggcgtaaa gacggctcac cgcctgtcag gcgcactttt tcggtgccca
     3601 gactggcgaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag cctttattgg
     3661 tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag ttacacacat
     3721 cggtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc agttgtgaag
     3781 ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc ctggtggcaa
     3841 tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg cagaggctag
     3901 agtgtttagt ggttatgccg atactagcgt gtaaatgaga tttttaccat ccacactttc
     3961 gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa taagggaaaa
     4021 tggcggcttt actttgatat acatcatcag ggaggcatag cgtgcatcgt cctttagagg
     4081 tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc cccgcaattt
     4141 gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc gtacgctggc
     4201 tgatcttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg ttctctcatc
     4261 actttcgtct ttgggttctc gtttaacggg tatcgtcacc accaccgata atggtggctc
     4321 gacggggcgt attcgccgtt cagaaggcgg cattgcctgg ggcgatatgc gcaactgcct
     4381 caaccagctg ataacggaac cgagcgtcgc ctccgcgatg tttgaatacc gttttggtgg
     4441 caatggcgaa ctttccggtc ataatctcgg aaacttgatg ttaaaggcgc tggatcacct
     4501 tagcgtgcgg cctctggaag ccatcaattt aattcgtaat ctgctgaaag tggatacgca
     4561 tttgattcca atgtcagagc atcctgttga tctgatggcg attgacgatc aggggcatga
     4621 agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaagagt tattgttaac
     4681 gcctaatgta cccgcaacgc gtgaggcggt tcacgctatc aatgaagcgg atctcatcat
     4741 tattgggcct ggcagttttt ataccagcct gatgccaatt ctgctgctga aggaaatcgc
     4801 ccaggcatta cgccgcacgc cagcgccgat ggtttatatc ggcaatctgg ggcgtgagtt
     4861 gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg agcagtatgt
     4921 tggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct cggcggtgaa
     4981 agagcggatt gtgatccagg aggtactgga ggccagcgat attccgtatc gtcatgaccg
     5041 ccagttgtta cataacgcgc tggaaaaggc gttacaggct ttaggttaac ggaaatccga
     5101 aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg gatgcggcgt
     5161 gaacgcctta tccggcctac aaaccgcgca aattcaatat attgcggaga aaatgtaggc
     5221 ctgataagcg tagcgcatca ggctgttttc cgtttgtcat cagtcttctt cgctatcctg
     5281 ttacgatgcc gcgataaaca gctcacgcag ctgatgcaac tggtcacgaa tttgcgccgc
     5341 ttcttcgaac tccagattct gcgcgtgttg catcatcaac ccttccagct catggatttt
     5401 ctgctgcaac gctttaggcg acatatccat cggcacatta tccggctcaa caatcggtcg
     5461 cgattttcct ctgcccttcg ctttggtttt agcaatgttc tgccccaacg ccaggatatc
     5521 gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt acttctgctg
     5581 tttctcgcgg cgacgttcgg tttcgccaat cgctttcgcc attgatgggg tgatcttatc
     5641 gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg tctggatcaa
     5701 cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca ccagcgaaac
     5761 ttccggcata tccagacctt cacgcagtaa gttgatccct accaatacgt cgaactcacc
     5821 cagacgcaag tcgcggataa tctccatacg ctcgacggtg tcgatatctg agtgaagata
     5881 acgcacgcgc tcaccgtgtt cttcgagata ttcggtaaga tcttccgcca ttcgcttggt
     5941 cagtgtggtg accagtacgc gttcgttaat tgccgctcgc tgacgaatct ccgaaagaag
     6001 atcatcaacc tgtgtcgcca ccggccgcac ttcgataatc gggtcaagca atccggttgg
     6061 acgcaccacc tgatccacca catcgccgcc ggatttttcc agctcgtaat tacccggcgt
     6121 cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaact taagcggacg
     6181 gttatccagc gctgatggca ggcggaagcc gtactccacc agtgtctctt tacgcgcccg
     6241 gtcaccgcga tacatgccgc caatttgtgg aatggtgacg tgagattcat cgaccaccag
     6301 cagcccatca gcagggaggt aatcaaacag cgtcggcggt ggctcacccg gtccacgacc
     6361 ggagaggaag cgcgagtagt tttcaatccc cgaacagtag cccagctcgt tcatcatctc
     6421 cagatcaaac tgggtacgct gggtcagccg ctgctcttcc agcagtttgt tgttttccaa
     6481 cagcactttg cgtctggcgg ccagctcttc tttgatctcc tccatcgcct gtacgatgcg
     6541 ctcgcgcggt gtgacgtagt gcgttttcgg gtagatggta aaacgtggaa tagtggaaac
     6601 aatctgcccg gtcagcgggt caaataacga caatcgttcc acttcctcgt caaacagttc
     6661 cacgcgaagt gcaatgtcat ccgattctgc cgggaagata tctatcacct cgccacgaac
     6721 gcggaaagta ccacgctgga atgcttgatc attacgagcg tattgcagct ccgccagtcg
     6781 gcgcagaatc gcgcgctgat cgataatcat accgaccgtg agatggagca tcatcttgag
     6841 atataaatca ggatcgccca gaccataaat cgcggaaaca gacgccacca caaccacatc
     6901 acgccgctcc agcatcgctt tggtggcgga caaacgcatc tgctcaatat gttcgttaac
     6961 cgaggcatct ttctcaatga aagtgtcgga actcggtaca taggcttccg gctgatagta
     7021 gtcgtagtag gaaacgaaat attccaccgc gttttccggg aagaactctt tcatttcgcc
     7081 atacagctgg gccgccagcg ttttgttggg cgcaagtacc atggttgggc gctgaaggtc
     7141 agcaatgaca ttggcaatgg tgaaggtttt cccggagcca gtcacgccaa gtaacgtctg
     7201 gtgcgccagg ccatcttcca gcccctcttc gagacgtcga atcgcctctg gctgatcgcc
     7261 agaaggttta aaagcggaat tcagtttgaa cggtttactc atgagtcgct acctgaagga
     7321 gttgggcggg caggtatgta attttactcg tcgtacttaa ttatgccaac aaattatact
     7381 ggataaaaaa acagttcatc accataatat ttctgttaca gcgtaaactc cgctgtcaat
     7441 catgagcaaa atttactctg tggcgagata aaactccggc cttaccgggt tatccccaaa
     7501 gcaacggctt ttttaacatt tgtcaagatg agtgatgaca gttttatggc aagagatgcc
     7561 tgttcagtga ctgccattgc atttatctaa ccagttaaaa aataaaagat atttctttga
     7621 gccgtcttta acgccaattc gcgtgcaagc cgcgtattct ctcgcttgcc tcgtgttttc
     7681 taactctaat acacatggtt atccacagga atagtggata actgtctcca gcccctatcc
     7741 cccgccgcct gggcaattcc cacattcgcc gcatgaggcg gaagtaaaat ttttttcgtg
     7801 attattttag aatttaattg gttaaattgc agtcaatcga agacgcgatc tcgctcgcaa
     7861 tttaaccaaa tacaggatag ctacaacaag gcaaggttta tgtactttcc ggttgccgca
     7921 ttttctggat tttctgcaag ccaggggatc tctcccagca gcggcgcggg aatcatgcgg
     7981 gtgagcgtgg tcatatattc agcgtgacgt tttcccggag gcgtaacatc gttcgccacc
     8041 caacccgcca gagtcagtcc ggcgtgttgt attgcctgcg cagtcaacat cgcgtgatta
     8101 atacagccga gtttcacacc aactaccagt atcaccggca gttgttcctg tgttacccaa
     8161 tctgcaaaag tgaaagtgtc agaaagcggc gtaaaccagc cgccagcacc ttccactaac
     8221 acccagtcag cctgttgttc aagcgcgcgt aatccggcgc tcattaccaa tgattctatc
     8281 ggcctgccct cttgcgcgct gatgatgtgc ggcgaggtgg gttctgcgaa ggtgtaagga
     8341 tttaccgttg cgtaatccag ctgcaggctg ctgttgtgct gtaacgccag cgcgtcacta
     8401 ttacgtaagc cttccggggt cttttcgctg ccagaggcga ccggtttata acctgccgtc
     8461 cgataaccta ctgccttcgc ggcttgtaaa agtgcacaac tggcgacggt tttccccact
     8521 tcggtatccg ttccggtgac aaaataacgt ttactcacga gcaatcactc ccaaaaaaag
     8581 atgatacgtc agaggatatc gcccctgttg ttgcggccag gccagttgca atcgctgcaa
     8641 ttgcgaacgc gttaatattc gcgggtcgcg cccttcatga agatgcgtag caccgatgcc
     8701 tttcagcgaa cgcatggcac tgagcgcatc atcaaaccac agcgtgatgg gctgaatatg
     8761 atgttgataa tgcacgccgt tcagcgactg ttcgatttca tctggcggta aaaagcgatt
     8821 agcatgcgga cgctcgtcca ccgcctgcca cgcctgatgc agttcgggta acgatccctg
     8881 caccagcgtg gtaaacgcga ccacgccttt ggggcgcacc acccgataca gctcgcggag
     8941 tgccgtggat aaattaccgc accactgcac tgcgagattg ctccatgcaa gatcgaacgt
     9001 cgcagtcgct aacggcaggg attcgatatc tcccgccaga taatggtctg cggcatcctt
     9061 ctggcgtgcc tgaacaagca ttggcggcga gagatctaag gccgtcacct gcgcgtgacg
     9121 ttcccgccag tggcggctca tccagccagg tccacaaccc gcgtccagta cgtgggtgta
     9181 tttacgctgt ggaagcattg ccagtaaggc gtcagcactc tggcgctgta gatctgcatg
     9241 ttgctcatag tgtgcggctg cccgaccaaa tgccgctgca atggcttgtt tattaaccgt
     9301 tgccatgcag cacctccagc agacggtcga tatcctgcat ttcatgcgca gcggttagcg
     9361 ttaagcgcag tcgcgcagta ccagcgggta cggttggcgg gcgaatcgcc gtgacccagc
     9421 agccttgctg gcgcagtttt tctgccagtt gtaacgcacg gctgttatca ccgacaatca
     9481 atggctggat ggcgctgcat gaatcagcaa gcgtaaacgg caaatcctgt actccggcac
     9541 gaaaacgcgt aatgagtgcc gccagttttt cgcgccgtgc atcaccctca tcactgcgaa
     9601 tgaccgccag cgacgcacgt aatgcctgcg cctgagcggg cggcatactg gtgctgtaga
     9661 taaggtggcg ggcgaattgc agcagataat ccgccaccgt actggagcaa agcaccgctg
     9721 ccccgctgac gccaaatcct ttgccaaaag tcactaccag caattctggt tttacctttt
     9781 gcagccagca gctgccgcgc ccctgctccc cgataacgcc cgtgccgtgg gcatcatcga
     9841 ccatcaacca gccattgtgc tgttgcgtta cctgctggat ttccgccagt ggcgcactat
     9901 cgccgtccat gctgaacacg ccttctgtca ccaccatttg ctgccccgga cagggggaag
     9961 caagcaatcg cgccaaatga gtgacatcgt tatgagcaaa acggcgaagc tgcgacgggc
    10021 ttaaactggc agcttccagc aatgaggcat ggctaagccg gtcggcagca atacggtcct
    10081 ctttcgccat catcgcggca ataactgcct gattagcggc gaaaccagag ataaacagca
    10141 gtgcccgcga atagccaagc cactcggcca gctcttcttc cagtgcctga tgcaccacgc
    10201 tataaccgct gacgtgaccg gagccgccgc taccgatgcc aaattgctcc gccccctgct
    10261 gccaggcacg gataatttgc ggatgatggc ttaaacctaa ataatcgtta ctggaaaagt
    10321 tcagatactg gcgatcatcc gccaccagcc agcgtccggc tccttgcgcc accggataac
    10381 ggcgacgcag ggcatcggca gcacgccgcg catcgagcgc cgcgttgatt ttctcctgcc
    10441 agctcataat gctgccgcgt tgtaatattc gtcggtgtcc ggggtcatca gcgcctgttc
    10501 aagacgttgc tgttgttcgt tatcccctgc cagcacggca gtttgctgcg gatttagccc
    10561 cagtttgcgg aacagttgca ggtctttatc ttcttccgga ttcggcgtgg tcagcagttt
    10621 gcaaccgtag aaaatcgagt ttgcgcctgc cataaagcac atcgcctgag tctgttcgtt
    10681 catctgctcg cgtccggcag aaaggcgcac gtaagaggtt ggcatcatga tccgcgcgac
    10741 cgcaatggtg cgaataaaat caaaggcatc gacatcatcg ttatcggcaa gcggcgtgcc
    10801 tttcaccttc accagcatgt tgattggcac gctttccggc ggcgtcggca ggtttgccag
    10861 ttgcagcaat aatccggcgc gatcttttac cgtttcgcct aagcccacaa tgccgccaga
    10921 acagaccttg atcccggcat cgcgcacttt ttccagcgta tcgaggcgtt cctgataagt
    10981 gcgtgtggtg atgatattgc cgtaaaactc cggcgaggtg tccaggttgt ggttgtagta
    11041 atccagcccg gcgttcgcga ggcgctgcgc ctgagattca ctcaacgtgc ccagcgtcat
    11101 acacgcctcc agccccatcg cttttacccc ctgcaccatt tgttccaggt acggcatatc
    11161 gcgttcgtgg ggattcttcc acgccgcgcc catacagaag cgcgtcgatc ctgccgcttt
    11221 cgctttgcgc gccgactcca gcacctgttc aacttccatc aaccgctcgg cttccagccc
    11281 ggttttgtag cgcgagcttt gcgggcagta tttgcaatct tccggacaag ctccggtctt
    11341 aatcgacagc aacgtgctga cctgcacctg acgaggatcg aaatgctggc gatgcacctg
    11401 ctgcgcttca aacagcagat ccagcaacgg tttttcaaat aattctgtga cttgcgacaa
    11461 tgtccagcgt gggcggtgag ccatggggct tctccaaaac gtgttttttg ttgttaattc
    11521 ggtgtagact tgtaaaccta aatcttttca atttggttta caagtcgatt atgacaacgg
    11581 acgatcttgc ctttgaccaa cgccatatct ggcacccata cacatccatg acctcccctc
    11641 tgccggttta tccggtggtg agcgccgaag gttgcgagct gattttgtct gacggcaaac
    11701 gcctggttga cggtatgtcg tcctggtggg cggcgatcca cggctacaat cacccgcagc
    11761 ttaatgcggc gatgaagtcg caaattgatg ccatgtcgca tgtgatgttt ggcggtatca
    11821 cccatgcgcc agccattgag ctgtgccgca aactggtggc gatgacgccg caaccgctgg
    11881 agtgcgtttt tctcgcggac tccggttccg tagcggtgga agtggcgatg aaaatggcgt
    11941 tgcagtactg gcaagccaaa ggcgaagcgc gccagcgttt tctgaccttc cgcaatggtt
    12001 atcatggcga tacctttggc gcgatgtcgg tgtgcgatcc ggataactca atgcacagtc
    12061 tgtggaaagg ctacctgcca gaaaacctgt ttgctcccgc cccgcaaagc cgcatggatg
    12121 gcgaatggga tgagcgcgat atggtgggct ttgcccgcct gatggcggcg catcgtcatg
    12181 aaatcgcggc ggtgatcatt gagccgattg tccagggcgc aggcgggatg cgcatgtacc
    12241 atccggaatg gttaaaacga atccgcaaaa tgtgcgatcg cgaaggtatc ttgctgattg
    12301 ccgacgagat cgccaccgga tttggtcgta ccggcaaact gtttgcctgt gaatatgcag
    12361 aaatcgcgcc ggacattttg tgcctcggta aagccttaac cggcggcaca atgacccttt
    12421 ccgccacact taccacgcgc gaggttgcag aaaccatcag taacggcgaa gccggctgct
    12481 ttatgcatgg gccaactttt atgggcaatc cgctggcctg cgcggcagca aacgccagcc
    12541 tggcgattat cgaatccggc gaatggcagc agcaggtggc ggctattgaa gtgcagctgc
    12601 gcgagcaact ggcaccagcc cgtgatgccg aaatggttgc cgatgtgcgc gtactggggg
    12661 caatcggtgt ggtcgaaacc actcgtccgg tgaatatggc ggcgctgcaa aaattctttg
    12721 tcgaacaggg tgtctggatc cggccttttg gcaaactgat ttacctgatg ccgccctata
    12781 ttattctccc gcaacagttg cagcgtctga ccgcagcggt taaccgcgcg gtacaggatg
    12841 aaacattttt ttgccaataa cgggaagtcc gcgtgagggt ttctggctac actttctgca
    12901 aacaagaaag gagggttcat gaaactcatc agtaacgatc tgcgcgatgg cgataaattg
    12961 ccgcatcgtc atgtctttaa cggcatgggt tacgatggcg ataatatttc accgcatctg
    13021 gcgtgggatg atgttcctgc gggaacgaaa agttttgttg tcacctgcta cgacccggat
    13081 gcgccaaccg gctccggctg gtggcactgg gtagttgtta acttacccgc tgatacccgc
    13141 gtattaccgc aagggtttgg ctctggtctg gtagcaatgc cagacggcgt tttgcagacg
    13201 cgtaccgact ttggtaaaac cgggtacgat ggcgcagcac cgccgaaagg cgaaactcat
    13261 cgctacattt ttaccgttca cgcgctggat atagaacgta ttgatgtcga tgaaggtgcc
    13321 agcggcgcga tggtcgggtt taacgttcat ttccactctc tggcaagcgc ctcgattact
    13381 gcgatgttta gttaatcact ctgccagatg gcgcaatgcc atctggtatc acttaaaggt
    13441 attaaaaaca actttttgtc tttttacctt cccgtttcgc tcaagttagt ataaaaaagc
    13501 tgaacgataa acagaaaaca ctgtaaatat caatatgtta tgaaagattt agtctaaata
    13561 atagactgca taatactaca aaacacaaca tatccagtca ctatgaatca accacttaga
    13621 tggtattagt gacctgtaac agagcattag cgcaaggtga tttttgtcct cttgcgctaa
    13681 ttttttgtca acgcactggg ttagttgaat ttacaaccat gctcagtatc tcgataaacg
    13741 cagagaaatg atgcagtggt gggcggactg gctggacgaa aagacacagt aatcaaaagc
    13801 ggcaggccac ataaaaagcc tgccttatac cgctacgctg tcacttctca catcgatgat
    13861 ggtgtgtgtc accacgccgg cgacagtgat atcatccagg gcctcgcctt caatcgcttc
    13921 gccgtcttcg gttatcagcg accgccctct caacgtggca agttccgtcc cgccgccgtg
    13981 ctggatcaga acctgactac cctgccttgg tttcagggag atatccaaca caacgtaacc
    14041 gccagaccgc tcgaaaacta gcgtgtttgg gcctacattg cagatcgagt taacagacag
    14101 acgctgctca acgtagtcag acgccggaga cgggaacgcc atcagatgac cctgcccatg
    14161 ttggccatca tccacagcct gttttcgcta tgctctgccg ttttgtcgac aaagtacgtc
    14221 tgctctcttg atatccagtt gttagcctcc tcttctgaaa aatggatacc cctccgctgt
    14281 agttcggaga tgaaagaatc agtgtaaagg tattgataac ctttaggatt tatagaaatg
    14341 gaggctctga aagcctccgc gatggcgtta cgtctgatca tgtctcttac caaacaaaac
    14401 tgtatttata tacagtattg tttgcgttaa tcaacagtca atcattttat tgaataaaag
    14461 tgtaaggaga agcgatggta tattgtgcat tagcccctat gggagtatcg aaaatccaca
    14521 cctcactcaa accatctttt gtaattgacg cgttaggtcc cccatggctg catccataaa
    14581 gatataactt tgctttacca gttaagtcag atccttgccc agcagtaaag caggcgccac
    14641 cgataaagtc tgcccttgaa ctatcatgcg agtaacaccc aaagttagct gtaaccgtgc
    14701 catcatgaac atcgtgaact acacggccaa acgttccgta gtattcaccg ttcaaacgta
    14761 tccctacagt tccgtcatga gcggtagagc cgttattatt tggtaaatta tcaaaaccac
    14821 tccaacggcc agtacaacca atttcgataa accacggctt tatcccaaga ccattctgat
    14881 cggcatgata gttaaatgcg tcctgcatcc cgtattgagc agtgcaattc tgcataatac
    14941 agtttacacc taaagagtta aaggcattcc ttgtcacaga taaatcaaat atacaatttt
    15001 ttgcatataa gtat
//
LOCUS       DAPHJE010000108        14298 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPHJE010000108 DAPHJE010000000
VERSION     DAPHJE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.109"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            264..1322
                     /gene="citC"
                     /locus_tag="RH222_003463"
     CDS             264..1322
                     /gene="citC"
                     /locus_tag="RH222_003463"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV3798426.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1337..1633
                     /gene="citD"
                     /locus_tag="RH222_003464"
     CDS             1337..1633
                     /gene="citD"
                     /locus_tag="RH222_003464"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV3798427.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1630..2538
                     /gene="citE"
                     /locus_tag="RH222_003465"
     CDS             1630..2538
                     /gene="citE"
                     /locus_tag="RH222_003465"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV3798428.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2549..4081
                     /gene="citF"
                     /locus_tag="RH222_003466"
     CDS             2549..4081
                     /gene="citF"
                     /locus_tag="RH222_003466"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV3798429.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4085..4636
                     /gene="citX"
                     /locus_tag="RH222_003467"
     CDS             4085..4636
                     /gene="citX"
                     /locus_tag="RH222_003467"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV3798430.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4611..5489
                     /gene="citG"
                     /locus_tag="RH222_003468"
     CDS             4611..5489
                     /gene="citG"
                     /locus_tag="RH222_003468"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV3798431.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5540..7003
                     /gene="citT"
                     /locus_tag="RH222_003469"
     CDS             5540..7003
                     /gene="citT"
                     /locus_tag="RH222_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV3798432.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7117..7923
                     /gene="rna"
                     /locus_tag="RH222_003470"
     CDS             7117..7923
                     /gene="rna"
                     /locus_tag="RH222_003470"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV3798433.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8153..8563
                     /gene="rnk"
                     /locus_tag="RH222_003471"
     CDS             8153..8563
                     /gene="rnk"
                     /locus_tag="RH222_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV3798434.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8794..10032)
                     /locus_tag="RH222_003472"
     CDS             complement(8794..10032)
                     /locus_tag="RH222_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV3798435.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10253..10681
                     /gene="uspG"
                     /locus_tag="RH222_003473"
     CDS             10253..10681
                     /gene="uspG"
                     /locus_tag="RH222_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV3798436.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10802..12367)
                     /gene="ahpF"
                     /locus_tag="RH222_003474"
     CDS             complement(10802..12367)
                     /gene="ahpF"
                     /locus_tag="RH222_003474"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV3798437.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12538..13101)
                     /gene="ahpC"
                     /locus_tag="RH222_003475"
     CDS             complement(12538..13101)
                     /gene="ahpC"
                     /locus_tag="RH222_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV3798438.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13472..14218
                     /gene="dsbG"
                     /locus_tag="RH222_003476"
     CDS             13472..14218
                     /gene="dsbG"
                     /locus_tag="RH222_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV3798439.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3417 a         3773 c         3586 g         3522 t
ORIGIN      
        1 cacttaaacc tgcaggtcat ttcgtaatta cattaattaa aaaacttaaa accattaaat
       61 aaataaaaaa ccactaactc tatgtgaaat aaatcaaaat ttcacgcaga aatactcctt
      121 aggatgtata gcgaaaggag aaaaagatat acctcgatca cccccctttc tcccaagtga
      181 aaataaaagg ttatcagttt gcaacattga acaacattcg ttgcaaatcg ataacaacat
      241 gcaccttcag gatactattt attatgttcg gcaatgatat tttcacccgc gtaaaacgtt
      301 cagaaaataa aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg
      361 acaccacagt cgaagtattt attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg
      421 gaattgccgg aaatattatt aaatgcgttg cgatcagtga atccgttcgc ggtgaaggac
      481 tggcgctgac attagccact gaactgataa acctcgccta tgagcggcac agcacgcatc
      541 tgtttattta taccaaaacc gaatacgagg cgctgttccg ccagtgcggt ttttccacgc
      601 tgaccagcgt acccggcgtg atggtgctga tggaaaacag cgccacgcga ctgaaacgct
      661 atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc attgtgatga
      721 acgccaatcc cttcacgaat ggtcaccgtt atctgattca acaagctgcg gcacagtgcg
      781 actggttgca tctgttttta gtcaaagaag attcttcacg cttcccctat gaagaccggt
      841 tggatctggt gttaaaaggc accgccgata ttccacgcct gactgtgcat cgcggctccg
      901 aatacatcat ctcccgcgct acgttccctt gctacttcat taaagaacag agcgtcatta
      961 accattgtta caccgaaatt gatctgaaga ttttccgtca gtacctcgct cccgcgctgg
     1021 gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc
     1081 aggatatgcg ctactggctg gaaacgccga ctatctccgc accgcccatc gaactggttg
     1141 aaattgagcg gctgcgttac caggagatgc cgatatccgc ttcccgggta cgtcaactgc
     1201 tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt
     1261 tgcagaacct gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag acccccgcat
     1321 gagaaacagg tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc ttgagtctgg
     1381 ggatgtgatg atacgcatcg ccccactcga tacgcaggat atcgacctgc aaatcaatag
     1441 cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc attctggacg ttctcgcccg
     1501 ctacaacgtg cgcggcgtac agctgaatgt cgatgataaa ggcgcactgg actgcatttt
     1561 acgtgcacga ctggaagccc tgctggcacg cgccagcggt atcccggctc tgccatggga
     1621 ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg ccgcagcatg
     1681 ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta cccggctgat
     1741 gccctgatgt ttgacctcga agactccgta gcattgcgtg aaaaagacac cgcccgccgc
     1801 atggtttacc acgcgctgca acatccgctg tatcgcgata ttgaaaccat tgtgcgtgtc
     1861 aacgcgctgg attccgaatg gggtgtcaac gacctggaag ccgtcgttcg cggtggtgcg
     1921 gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg ttctggatat cgaaaaagag
     1981 atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca gcaccggcct gctggcggcg
     2041 attgaatctc cgctgggcat tacccgcgca gtggaaatcg ctcacgcttc cgagcgtttg
     2101 atcggtatcg ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga acgctccccg
     2161 gaaggaactg aactgctgtt cgcacgctgt tccattttgc aggccgcgcg ctctgcgggt
     2221 attcaggcgt tcgataccgt ctattccgac gctaacaacg aagccggatt cctgcaagaa
     2281 gccgcccaca tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc gcgtcagatt
     2341 gatctgctgc acaacctcta cgcaccgacc cagaaagaag tggatcacgc ccgccgcgtc
     2401 gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct gaacggcaag
     2461 atggtggacg gtccggttat cgatcgcgcc cgtctggtgc tctcccgtgc agaactttcc
     2521 ggcatccgcg aagaataagg caatcaaaat gacgcagaaa attgaacaat ctcaacgaca
     2581 agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat cttgccgctt tccagaactc
     2641 gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc aaactgtgcg ccaacctgga
     2701 agaggcgatt cgtcgctctg gtttacagga cggcatgacg gtttccttcc atcacgcttt
     2761 ccgtggcggt gacctgaccg tcaatatggt gatggacgtc atcgcgaaga tgggctttaa
     2821 aaacctgacc ctggcgtcca gctccctgag tgattgccat gcgccgctgg tagaacacat
     2881 tcgccagggc gtggttaccc gcatttatac ctctggccta cgcggtccac tggcggaaga
     2941 aatctcccgc ggtctgctgg cggaaccggt gcagatccac tctcacggcg gtcgtgtgca
     3001 tctggtacag agcggcgaac tgaatatcga cgtggctttc ctcggcgtcc cgtcctgtga
     3061 tgaattcggt aatgccaacg gctacaccgg taaagcctgc tgcggctccc tcggctatgc
     3121 aatggttgat gccgacaacg caaaacaggt tgtgatgctt accgaagaac tgctgcctta
     3181 tccgcataat ccggcaagca ttgagcaaga tcaggttgat ttaatcgtca aagttgatcg
     3241 cgttggcgat gctgcaaaaa tcggcgctgg tgctacccgt atgaccacta acccgcgcga
     3301 actgcttatt gcccgtagcg ctgcggatgt gattgtcaac tcaggctact tcaaagaagg
     3361 tttctccatg caaaccggca ccggcggcgc atcgctggcg gtaacccgtt tcctggaaga
     3421 caaaatgcgt agccgcgata ttcgcgccga cttcgccctc ggtggtatta ccgcgacgat
     3481 ggttgatctg cacgaaaaag gtctgatccg caaactgctg gatgtgcaga gctttgacag
     3541 ccatgctgcg caatcgctgg cccgtaaccc caatcacatc gaaatcagcg ccaaccagta
     3601 cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc gacgtggtgg tactgagcgc
     3661 gctggaaatt gacacccagt tcaacgttaa cgtgctgacc ggctctgacg gcgtactgcg
     3721 tggtgcttcc ggtggtcact gcgataccgc gattgcctct gcgctttcca tcatcgtcgc
     3781 gccgctggta cgcggtcgta ttccgactct ggtggataac gtgctgacct gcatcacccc
     3841 gggctccagt gtcgatattc tggtcacaga ccacggtatc gcagttaacc cggcacgtcc
     3901 ggaactggca gaacgtctgc aggaagcggg cattaaagtg gtttccattg agtggctgcg
     3961 cgaacgtgcg cgtctgctga ccggtgaacc acagccgatt gaattcaccg accgcgtcgt
     4021 tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt gtgcatcagg tgaaggaata
     4081 agccatgcac ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc ccgagctgct
     4141 cgtcagccgg gatgaaaggc aagcacggca acacgtctgg ctcaagcgcc atcctgttcc
     4201 actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa gacagcgagg tcacacgccg
     4261 aatttttaat catggcgtga cagccttgcg tgccttagcc gcaaaacagg gctggcaaat
     4321 tcaggagcag gctgcactgg tttccgccag cgggccggag ggcatgttga gcattgccgc
     4381 cccggctcgc gacctcaagc tcgccaccat tgagcttgaa catagtcatc ctctcgggcg
     4441 gttatgggat atcgatgtcc tgacgcccga aggcgaaatt ctctcccgcc gcgactattc
     4501 actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca gccgtctgcg cgcgtggaaa
     4561 aacccatcaa ctgaccgatt tactcaaccg catggaggca ctgctgaacg atgtcgatgc
     4621 ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt aatcgatgag tacgccctgc
     4681 tgggctggcg cgccatgctg actgaagtca atctgtcacc gaaaccaggc ctcgtggatc
     4741 gcattaactg cggtgcgcac aaagatatgg cgctggaaga tttccaccgc agcgcgctgg
     4801 cgattcaggg ctggttaccc cgtttcattg aatttggtgc ctgtagtgca gaaatggcac
     4861 cggaagcggt actccacgga ttacgcccaa ttggtatggc ttgcgaaggt gatatgttcc
     4921 gcgccactgc gggcgtaaac acgcataaag gcagcatttt ttctttaggg ctgctatgtg
     4981 cggcaattgg ccgtttgctt cagctcaacc agccggtaac gccaacaacc gtttgttcta
     5041 cggcggcaag tttctgccgt ggcctgaccg atcgcgaact gcgtaccaat aattcacaac
     5101 tgacggcagg tcaacggttg taccagcagc ttggccttac cggcgcacgc ggtgaagccg
     5161 aagcgggtta tccactggtg atcaatcacg ccttgccgca ttacctcact ctgctggatc
     5221 aggggttaga tcctgaactg gcattgctcg ataccttgct cctactgatg gcgatcaacg
     5281 gcgataccaa cgttgcatcg cgcggtggcg aggggggcct gcgctggcta cagcgcgagg
     5341 cgcaaacatt attgcaaaaa gggggcattc gaacccccgc cgatctcgat tatctccggc
     5401 agttcgacag ggagtgtatc gaacgaaatc tcagtccagg cggcagtgcc gacctgctga
     5461 tccttacctg gtttttagca cagatttaat tatttaagca cttgataaat ttggaaatat
     5521 taattttcgg agaacccgta tgtctttagc aaaagataat atatggaaac tattggcccc
     5581 actggtggtg atgggtgtca tgtttcttat ccctgtcccc gacggtatgc caccacaggc
     5641 atggcattac ttcgctgtgt ttgtggcaat gattgtcggc atgatcctcg agccaattcc
     5701 ggcaacagcg atcagtttta ttgcggttac tatttgcgtt attggcagta attacctgct
     5761 ctttgatgcc aaagaattag ctgacccagc gtttaatgcg caaaaacagg cgctgaaatg
     5821 gggtctggct ggtttttcca gcaccacggt atggctggta tttggcgcat ttatttttgc
     5881 attagggtat gaagtttccg ggttaggtcg tcgcattgcc cttttcctgg tgaaattcat
     5941 gggcaaacgc acgctgacgt tgggttatgc gattgtcatt atcgacattc tgctggcacc
     6001 gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt tttccggtca ttaaaaacct
     6061 gccgccgctg tttaaatcat tccccaacga tccgtccgcg cgtcgtattg gcggctattt
     6121 gatgtggatg atggtcatta gtaccagtct gagttcgtcc atgtttgtca ccggtgcggc
     6181 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6241 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6301 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6361 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6421 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6481 tctgctggca gtttcgctaa tgctggccct gcacgttgtg ccttggaaag atattacccg
     6541 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6601 cctgactcgt tctggcttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6661 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6721 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6781 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     6841 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     6901 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     6961 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7021 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7081 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg gcgtaacgcc
     7141 gcgttgctcg cggtttctct gcttcccttc tcttctgcca acgccttagc gttgcaggca
     7201 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7261 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7321 accaacaaag ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7381 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7441 ccgaatctac cagaagcgca cgccagccgg atgtgttcat cgccggaaac cggattatca
     7501 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7561 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7621 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7681 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7741 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7801 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     7861 ttgccacaac cacacccagg caactgtggc aaaacctttg tgattgataa agtgggttat
     7921 taatcatctg atttacaaaa cacgctaatt cgtcttccat aaaccgtgac gattaagaat
     7981 tatacataat gcgctgcatt gaggaattcc ctgaatgtga cgcaaatcac ttcaaagagt
     8041 gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt gagggttttc
     8101 ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa aaatgtccag
     8161 accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc tggagcaacc
     8221 cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc gcgcccaaat
     8281 gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg ttaaattccg
     8341 caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa aaatgaccga
     8401 tagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg ggctgcgcgt
     8461 tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg aagtgctgga
     8521 actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc tgcacaagcg
     8581 ataactcgct ttgcccagag gatgcactcg catcctcttt acgcaaaatc agcaattttt
     8641 cctggttctc actgttcact tttgctgcaa aattattccg tttcgtttgt gggaataacc
     8701 atggcagaag cattttacat cctgatagga tttctgatta tggcggcgat tatcgtcatg
     8761 gctgtccttt acctcgaaaa ccactcctga cgatcatatt gttccccccg gcatcgcatt
     8821 caccagacct gaaaccgcct tctgcgccgc ctctgcgctt tgtgcaccgg gtaccagaat
     8881 caccttacgg cactcctctt cacgtttttc gaaaatctca tatccccggg cggcctcttc
     8941 aaacggcata tagtgggtaa caatttcttc tggtttcagt aatcctttct caattaacgg
     9001 taataattct cccagccatg cgtgaacgtg ggtctgtccc attttaaacg tcaacccttt
     9061 atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca gcgtagacgc ccggtacgct
     9121 aacaatgccg ccacgcctga ccgccgcaat acactggcgc aacgctttac cgctgctgcc
     9181 ctccagtttc aggttagtca gcaccgtttc cgtggtgctg cctttcgctt caaaaccgac
     9241 ggcgtctatt actgcatcca cgccccggtg acctgccgtt tgttcaataa ttgactgtgc
     9301 cggatcgcta tcttcatcga aattaatcgg gatcgcgccg tagcggtcag cggcgaaacg
     9361 caagcggtag ggatgatgat caacaacaaa aatctgttcc gcaccgagca accgtgcaca
     9421 ggcgattgtc aacaatccca ctggaccggc accatagact gcaacgcttg aaccttgttg
     9481 gatctgcgca ttttttgctg cctgccatgc cgttggcaga atatcagaaa ggaaaagggc
     9541 tttatcatct gaaagcaaag gcggtacttt aaacggcccc acattccctt tagggacgcg
     9601 gacatattcc gcctgtccac caggaacgcc accatacagg tgactataac caaacaatgc
     9661 cgcgggagct ggtatctgtt ttttattgag cgcagcgcct ttacccgcat tggtattttc
     9721 gcaggcggca tattgctgca ttcgacagaa aaaacagtcg ccacaagcaa tgacaaacgg
     9781 aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc tttccggttt caacgacttc
     9841 ccccataaat tcatgaccaa aaatatcgcc atgtttaacc tggggtattt tgcctcgata
     9901 aagatggagg tcagagccac agatcgccgt tgccgtaata cgcagaataa tatcatctgc
     9961 ctgttcaatg cccggatcgg gaacattttc tacctgaacg tgatgtgggc cgtgataagt
    10021 caatgctttc atgcaacctc cagagacagt tcaaaaaaga gtcagtcttt aagggtagct
    10081 atcacagcgg gaaaacgggc ggtaccgcag gttttgggag aattcctggt gttcagtgag
    10141 aatatattaa tcacagaaac cgttcgtaag gaaaaagaac cagacaaata tcagggataa
    10201 atattataat catggcgaat gatttgttca tgattaacag ggagaaaggg ttatgtataa
    10261 gacaatcatt atgccagttg atgtatttga aatggaattg agcgacaaag ctgttcgcca
    10321 cgctgaattc ctcgcccagg atgacggagt tattcatcta cttcacgtac tacccgggtc
    10381 agccagcctg agcctgcacc gttttgccgc tgatgtgcgt cgttttgaag agcatctgca
    10441 acatgaagca gaagaacgtc tgcaaacgat ggtcagccac ttcaccatcg atccttcccg
    10501 cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa gtcaatgagt tggcagaaga
    10561 actgggggct gatgttgtag ttattggttc tcgcaaccca tcgatttcga cccatctgtt
    10621 aggttctaac gcctcgagcg tgatccgcca tgccaatctg ccggtgctgg ttgtgcgtta
    10681 attgccagaa taaatatccc gccctgcccg gagccatccc ggcagggctt tttaatgcca
    10741 taaaaaagcc gccaggtttg accccggcgg ctaagcaatt gcaggtgaat cttacttctt
    10801 cttatgcagt tttggtgcga atcaggtagt caaaagcact cagagaggct ttggcacctt
    10861 cgccagtggc gatgataatc tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca
    10921 cgcctttgac gttggtttcg catttcgcat caatgataat ctcacccatg cggttacgtt
    10981 cgactgcgcc ttccagccag ttggtgttcg gcagcagacc aatctggacg aaaataccgg
    11041 ccagttcgat gttgtgaata tcgccgctga cacgatctcg atattccaga ccaacgactt
    11101 tgctgccgtc gcctttcact tccgtggttt gcgcattcag aataatgtcg acgtttttca
    11161 ggctgcgcag tttgtcctgc agaacctggt cggctttcat ttctggcgca aattcgagca
    11221 gcgttacgtg ctcaacgata cccgccaggt cgattgccgc ttccacgccg gagttaccgc
    11281 cgccgataac cgctacgcgt ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg
    11341 tcacgccttt ggtgcgatac tgatcttcgc ccggaacgtt catgttgcgc cattttgcac
    11401 cggtcgccac gataatgctg cgtgctttca gtaccgcgcc agaagctgtt tcaatctggt
    11461 gtagaccacc ttcaaccgct gccgggatca gtttgctggc gctctggctg tcgatcacat
    11521 caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc cagtttctgc ccttcagtct
    11581 tcggtacaga aatgtagttt tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt
    11641 cgcccatcag accggtacgg atgcctttac gtgcggagta aattgctgcc gctgcacccg
    11701 ccgggccgga accgacgatt aatacgtcat aagcatcacg cttgttcagc tcttctgccg
    11761 cacgtttttc cgcgccagta tcaattttgg caacgatttc agtcaacgtc atgcggccct
    11821 gaccaaactc tttcccgttt acgaacactg ccggaacgcc catcacgttg cgatcggtga
    11881 tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt gatgcgcggg ttcaatacgc
    11941 tcatcaggtt cagcgcctgc accacgtccg ggcagttatg gcaagagagc gagtaatagg
    12001 tttcgaattc aaaatcaccg tcaatatggc gaatctgctc cagcagagac tgcgcttctt
    12061 tcgacggatg accaccggtc cacagtaacg ccagtaccag cgaggtgaac tcgtggccca
    12121 gcggggagcc tgcaaaacgt ggcccctggt tggaacctgg gttggtgatc aggaaagacg
    12181 gcttacgcac cggcaagctg ttatcttctt taaaggtgac tttgtctgac agttctgcga
    12241 tttcagccaa cagttccttg atttctgccg atttagcgct gtcatccaac gtggcaatta
    12301 actcaacagg cttggtcaat ttctcaaggt aagccttgag ttgagttttc atatttgtgt
    12361 cgagcatgtt tatctcctgg gctttaaaca tcatcatgca agcggcctct gcggctacct
    12421 ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt
    12481 aggtgaattt ccgaagtatt tcgcgccgga gttggtagcg cgaaagacga aggaaattta
    12541 gattttacca accaggtcca gagacggagc cagagttgct tcaccttctt tccatttagc
    12601 cgggcaaact tcacctgggt gagaagctac gtactgtgct gctttgattt tacgcagcag
    12661 gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg attgcctgga tgataccctg
    12721 cgggtcaaca acgaaggtcg cacgatcagc cagaccttca tcttcacgca tgttgtcgaa
    12781 gttacgggtc agggcgccag tcgggtcgcc gatcatcgca tatttgattt tagcgatggt
    12841 ttcagagctg ctgtgccatg ctttgtgggt gaagtgagta tcggtagata ctgcgtatac
    12901 gtctacgccc agtttctgca gttcttcgta gtggtcagca acgtcaccca gttcggtcgg
    12961 gcatacgaaa gtaaagtcag ccgggtagaa gaagaagacg ctccagcggc cttctgtatc
    13021 tttttcggtg atttcgatga attcgccgtt tttgaatgcc tggtttttaa aaggtttaat
    13081 tttggtgtta atcaaggaca tctatacttc ctccgtgttt tcgatgagat gtaaggtaac
    13141 caatttttgc cgattcggct aatgcgtttc cattatcaaa tcgataagtt ttaccttaca
    13201 accttcgtaa gacaactttg tgaactttgc accgccagcg gcgataaaaa gtaaaatggc
    13261 cccctttcca ggtgaccgtg taatctgaac taccctttgg tatcttcagc gactgcctgc
    13321 aggctgagtg ttgcccttac aaatgcaaca acgacatgga ttacaacacc ctcataaaca
    13381 aagggcaatc acctgatcta agctcttacc tatgacagtg ataggttatg ccttttactc
    13441 gacttttgca ctgactgaaa aggacaaatt aatgttaaaa aagatacttt tactggctct
    13501 gcttcctgca atcgccttcg cagaggaact tcctgctcca gtaaaagcga ttgaaaaaca
    13561 gggcattaca atcatcaaaa cgttcgatgc ccccggagga atgaaaggtt atctcggaaa
    13621 gtatcaggat atgggcgtca ccatctacct gactccagat ggtaagcacg ctatctctgg
    13681 ttacatgtac aacgagaaag gtgaaaacct gagtaacaca cttatcgaaa aagaaattta
    13741 cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa tcccactggc tcctcgacgg
    13801 taaaaaagat gcgccggtca ttgtctacgt cttcgccgat ccgttctgcc catattgtaa
    13861 acagttctgg cagcaggcgc gcccgtgggt agattctggc aaagtgcaat taagaacatt
    13921 gttggttggg gttatcaagc cagaaagccc ggcgacagca gcggcaattc ttgcctccaa
    13981 agatcccgca aaaacctggc aacaatatga agcctctggt ggcaagctta agctaaacgt
    14041 gcctgcaaac gtaagtacag agcaaatgaa agtgttaagt gacaatgaga aactgatgga
    14101 cgatctgggg gcaaatgtca cgccggctat ctattacatg agtaaggaaa atacgctaca
    14161 acaggccgtg gggttgcccg atcagaaaac gcttaatatc attatgggga ataaataaaa
    14221 gataattcat ggatggttga agtacatctc tgcttttcaa ccatcctaaa aaagcatgaa
    14281 ttgctcgctc tgccctaa
//
LOCUS       DAPHJE010000109        14020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPHJE010000109 DAPHJE010000000
VERSION     DAPHJE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.110"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..371
                     /locus_tag="RH222_003477"
     CDS             <1..371
                     /locus_tag="RH222_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3798440.1"
                     /translation="IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNK
                     ATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNN
                     ANNWYLTIIDDHGNYISDKI"
     gene            387..2867
                     /locus_tag="RH222_003478"
     CDS             387..2867
                     /locus_tag="RH222_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3798441.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSNYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGSVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYQENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            2883..3917
                     /locus_tag="RH222_003479"
     CDS             2883..3917
                     /locus_tag="RH222_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV3798442.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNIILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(3999..4337)
                     /gene="rcnB"
                     /locus_tag="RH222_003480"
     CDS             complement(3999..4337)
                     /gene="rcnB"
                     /locus_tag="RH222_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV3798443.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(4556..5380)
                     /gene="rcnA"
                     /locus_tag="RH222_003481"
     CDS             complement(4556..5380)
                     /gene="rcnA"
                     /locus_tag="RH222_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV3798444.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            5501..5773
                     /gene="rcnR"
                     /locus_tag="RH222_003482"
     CDS             5501..5773
                     /gene="rcnR"
                     /locus_tag="RH222_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV3798445.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5865..5961
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5996..6784
                     /gene="thiM"
                     /locus_tag="RH222_003483"
     CDS             5996..6784
                     /gene="thiM"
                     /locus_tag="RH222_003483"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV3798446.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            6781..7581
                     /gene="thiD"
                     /locus_tag="RH222_003484"
     CDS             6781..7581
                     /gene="thiD"
                     /locus_tag="RH222_003484"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV3798447.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            7646..8464
                     /locus_tag="RH222_003485"
     CDS             7646..8464
                     /locus_tag="RH222_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV3798448.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            8516..9262
                     /locus_tag="RH222_003486"
     CDS             8516..9262
                     /locus_tag="RH222_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3798449.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAEQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9236..10201)
                     /locus_tag="RH222_003487"
     CDS             complement(9236..10201)
                     /locus_tag="RH222_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3798450.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10198..11202)
                     /locus_tag="RH222_003488"
     CDS             complement(10198..11202)
                     /locus_tag="RH222_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV3798451.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(11199..12476)
                     /locus_tag="RH222_003489"
     CDS             complement(11199..12476)
                     /locus_tag="RH222_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HDV3798452.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            12733..13785
                     /gene="fbaB"
                     /locus_tag="RH222_003490"
     CDS             12733..13785
                     /gene="fbaB"
                     /locus_tag="RH222_003490"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV3798453.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAVTIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         3726 a         3319 c         3552 g         3423 t
ORIGIN      
        1 gcatttttta tctgaatgtt ctggatattc caccaaatag tccagagcaa gaaggtaaga
       61 atgcactgaa gtttgcgatg caaaacagaa ttaagttgtt ttaccggcca gcgggtattg
      121 ctccggtaaa taaagcgacc tttaaaaagt tactggtaaa tcgcagtggc aatggattgg
      181 tgataaaaaa tgactcagct aattgggtga cgatttcgga tgtcaaagct aataatgtta
      241 aagtcaatta tgaaactatt atgattgccc ccttagaaag tcagagtgtt aatatcaaaa
      301 gtaataatgc aaataactgg tatctgacca ttatcgatga ccacggcaac tatattagtg
      361 acaaaattta attgcaggag ctgcctatgt tgagaatgac cccacttgca tcagcaatcg
      421 tagcgttatt gctcggcatt gaagcttatg cagctgaaga aacctttgat acccatttta
      481 tgataggtgg aatgaaagac cagcaggttg caaatattcg tcttgatgat aatcaaccct
      541 taccggggca gtatgacatc gatatttatg tcaataagca atggcgcggg aaatatgaga
      601 ttattgttaa agacaacccg caagaaacat gtttatcaag agaagttatc aagcggttag
      661 gcattaatag cgataacttc gccagcggta agcaatgttt aacatttgag caacttgttc
      721 agggtgggag ctatagctgg gatatcgggg tttttcgtct cgatttcagt gtcccgcagg
      781 cttgggtgga agaactggaa agtggctatg ttccaccgga aaactgggag cggggtatta
      841 atgcgtttta tacctcttat tatgtgagtc agtattacag cgactataaa gcgtcgggta
      901 acaacaagag tacatatgta cgttttaaca gcgggttaaa tttactggag tggcaactgc
      961 attctgatgc cagtttcagt aaaacaaata acaatccagg ggtgtggaaa agcaataccc
     1021 tgtatctgga acgtggattt gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca
     1081 catcaagcga tatttttgat tctgttcgct tcagcggtgt gcggttgttt cgtgatatgc
     1141 agatgttgcc taactcgaaa caaaatttta cgccacgggt gcagggaatt gctcagagta
     1201 acgcgctggt aactattgaa cagaatggtt ttgtggttta tcagaaagag gttcctcctg
     1261 gcccgtttgc gattacagat ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg
     1321 tgaaagaggc ggacggctcg gtaaccacct atctggtgcc ttatgcagcg gtgccaaata
     1381 tgctgcaacc cggcgtgtcg aattatgatt ttgcggcggg tcgtagccat attgaagggg
     1441 cgagcaaaca aagtgatttt gtccaggcgg gttatcagta tggttttaat aatttattga
     1501 cgctgtatgg tggctcgatg gtcgcgaata attattacgc gtttactttg gggactggct
     1561 ggaatacacg cattggtgcc atttccgtcg atgccacgaa gtcgcatagt aaacaagaca
     1621 acggcgatgt gtttgacggg caaagttatc aaattgccta caacaaattt gtgagccaaa
     1681 cgtcgacgcg ttttggtctt gcggcctggc gttattcatc gcgtgattac cggacattta
     1741 atgatcacgt ttgggcaaac aataaagata attatcgccg tgatgaaaac gatatctatg
     1801 acattgccga ttattaccag aacgattttg gccgtaaaaa tagcttttct gccaatatga
     1861 gccagtcatt gccagaaggc tgggggtctg tgtccttaag tacgttatgg cgagattact
     1921 gggggcgtag cggcagcagt aaggattatc agttgagtta ttccaataac tggcggcgga
     1981 taagctatac cctcgcggca agccaggcgt atgacgagaa tcatcatgaa gagaaacgtt
     2041 ttaatatttt tatatcgatt ccctttgatt ggggtgatga cgttacgacg cctcgtcggc
     2101 aaatatatat gtctaactca acgacgtttg atgatcaggg gtttgcctca aataatacgg
     2161 gattatcagg aaccgttgga agccgggatc agtttaacta tggggtcaac ctgagttatc
     2221 agcatcaggg aaatgaaacg acagctgggg cgaatttgac ctggaacgcg ccggttgcga
     2281 cagtgaatgg cagttatagt cagtcgagta cttatcgaca ggctggagcc agtgtttcag
     2341 ggggcattgt cgcctggtcg ggtagcgtta atctggcgaa ccgtctttcc gaaacgtttg
     2401 ctgtgatgaa tgcgccagga attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa
     2461 caaaccgtaa tggagtggtg atatacgacg gaatgacacc ttatcaggaa aatcacctga
     2521 tgctggatgt gtcgcaaagc gatagcgaag cagaattacg tggcaaccgg aaaattgccg
     2581 ccccttatcg cggcgcggtt gtactggtta attttgatac cgatcagcgc aagccctggt
     2641 ttattaaagc gttaagagcg gatggtcaac cattaatgtt tggttatgaa gtcaatgata
     2701 tccatggtca taatattggc gttgtcggcc agggaagcca gttatttatt cgcaccaatg
     2761 aagtaccgcc atcggttaat gtggcaattg ataagcaaca aggactttca tgcacaatca
     2821 ccttcggtaa agagattgat gaaagtagaa attatatttg ccagtaatag tcaggtggtt
     2881 ctatggaaat tcgcataatg ctatttatat taatgatgat ggttatgcct gtgagctatg
     2941 cggcatgtta tagtgagtta tctgttcagc acaatttggt tgttcagggg gattttgcac
     3001 ttactcaaac acaaatggcg acatatgagc ataattttaa tgattcgtca tgcgtgagta
     3061 caaatactat cacccctatg agcccgtcgg atattattgt tggactttat aacgatacca
     3121 ttaaattaaa tttacatttt gaatggacca ataaaaacaa catcacgttg tcaaataatc
     3181 agaccagttt caccagtggt tattcggtaa cggtgacacc tgcggccagt aatgcaaaag
     3241 tgaatgtttc tgcggggggc ggcggttcag tgatgattaa tggagttgcg acattatcca
     3301 gtgcttcatc atcgacacgc gggagtgccg cagtacaatt tctactgtgt ttattaggtg
     3361 gcaagtcatg ggatgcatgt gtaaatagct acagaaatgc attggcacaa aatgcaggtg
     3421 tctattcctt taatctgaca ttgtcataca acccgataac cacaacctgc aaaccggacg
     3481 atttattaat tactttagac agtattcccg tttcacaatt accagcgaca ggtaacaaag
     3541 caacaataaa tagtaaaaaa gaggatatta ttctgcgttg taaaaattta ttaggtcaac
     3601 aaaatcaaac atcacggaaa atgcaggtgt atttatcaag ttctgacttg ttaaccaaca
     3661 gcaacataat actgaaaggt gcggaagata atggcgtagg atttattctt gaaagtaatg
     3721 gttcgccagt cacactttta aatatcacta acagcagtaa aggatacaca aatttaaaag
     3781 aaattgcggc aaagtcaaaa cttacagata caacggtttc aattccgata acagccagtt
     3841 actacgtcta cgatacaaat aaaattaaat ctggcgcact ggaggcaacc gcattaatca
     3901 acgtgaaata cgactaataa caagcaagac gagcaagtgg ctaataataa aaaataacaa
     3961 ggtgtctgca tttccccctc atgatgaggg ggcttttttt agcgatgata aaaaatctca
     4021 ccgtcgtagg ctttaatgat tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc
     4081 atataggtcc agtgcgtccc ggcatcaggc gcgggcaggt tacgtaactg ccactgctta
     4141 atgttgtact catcggtgcg gtacatctct gggacggtat cgcctggttt gaaatgctta
     4201 aagtcagcaa tgaaactgct taactcatac ttagaaattc ccgaggtatt ggtcgaaccc
     4261 gcggtggctg gtgcggccca ggcggcactg gtaaccagca aaagcgcacc cagcaacatc
     4321 ttatttttaa tggtcataaa ctctccactt tggggatctg taatatcagt cgcatatctt
     4381 tcctgccata caatataact gacaagcgat attttgttag tgatcccatg atttcgtaaa
     4441 aagtgtaaat gaaaggatgc cctaaaagca tccttagcga gcagagagtc agcggaaata
     4501 gctgatttaa gaatcatcct gggctgaaga taacatgcaa gcctaaagga ttgagttatc
     4561 gcattatgcc catgaagcca tgtacgccca tatacacacc gactaagcca atcaacagac
     4621 tggaaaaata gggggcgcgt ttagcgagag tgttaaatcc gctccagcgt tttgcgacct
     4681 gctgaacgct gattgctgcg ccaacgccta cggtgacaag cgttaacgcc aggccaatgc
     4741 tgaaactgac gaccagtgtt gcgcccagtg tcagggcttt caactgaatg catatcaaca
     4801 gcacggtaat tgctgccggg caggggataa ggccaccggt taagccaaat aacaaaattt
     4861 gccagttggt gacctctcta ccatcaaagc gtcgtttaat gtcattggca tgggctcgtg
     4921 catgggcatc ctgatactcg ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat
     4981 gttcgtgatc gtgatgatga tgctcataat catgcccgtg catattctcc agccagttgc
     5041 gttcgccgcg ccaggtacgc cagaacatcc agaacgcggt gctaataatg atcactgcgg
     5101 aaatcagctg gagccacggt tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc
     5161 cgccaaaggc aattaaccag accactgcgg tatgcgaaat agttgctgcc agtccgagca
     5221 tcaccgcttg tttaatggtg cctttgatgg cgatgataaa cgccgccatc atcgtttttg
     5281 agtgccctgg ttccaggcca tgaagcgcac caagtaagat ggcgctgggg atgaagaacc
     5341 aggcgtttcc ttgctgaaga agagttgtaa attcggtcat gataataatt cttagtatta
     5401 attcggcaat ctgattctac tcccccccag tacctgatac taccccccag tagattaata
     5461 gtgctatgat ttttcatatt cttgttaacc aggtgttgcc atgtctcata caatccgtga
     5521 taaacagaaa ctgaaagcgc gtgccagtaa gattcagggc caggtcgtgg cgctcaagaa
     5581 aatgctcgac gagccgcacg aatgcgctgc agttttacaa cagattgctg ctatccgtgg
     5641 cgcggtaaac ggtctgatgc gggaagtgat taaaggtcat ctgacggaac acatcgttca
     5701 ccagggggat gagctaaaac gtgaagaaga tctggatgtc gttctgaagg tgctggattc
     5761 atatatcaaa taatttatta acgcgattgt aaaactgccg tttttcctcg tttacaacgc
     5821 gtgcgctgga cattaccatc ctcctctgcg atttatcatc gcaaccaaac gactcggggt
     5881 gcccttctgc gtgaaggctg agaaataccc gtatcacctg atctggataa tgccagcgta
     5941 gggaagtcac ggaccaccag gtcattgctt cttcacgtta tggcaggagc aaactatgca
     6001 agtcgacctg ctgggttcag cgcaatctgc gcacgcgtta cacctttttc accaacattc
     6061 ccctcttgtg cactgcatga ccaatgatgt ggtgcaaacc tttaccgcca ataccttgct
     6121 ggcgctcggt gcatcgccag cgatggttat cgaaaccgaa gaggccagtc agtttgcggc
     6181 tattgctagt gcgttgctga ttaacgttgg cacgctgacg cagctacgcg cacagtcgat
     6241 gtgtgccgcc gttgagcaag caaaaagctc tcaaacaccc tggacgcttg atccagtagc
     6301 ggttggtgca ctcgattatc gccgccgttt ttgtctggaa cttctgtctc ataagccaac
     6361 cgccatacgt ggtaatgctt cggaaatcat ggcattagct ggcattgcta atggtggacg
     6421 gggagtggat accactgacg ccgcagctaa cgcgataccc gctgcacaaa cactggcacg
     6481 ggaaactggc gcaatcgtcg tggtcactgg cgagatggat tatgttaccg atggacatcg
     6541 tatcattggt attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc
     6601 attatcggcg gttgtcgctg cctgctgtgc attaccaggc gatatgctgg aaaatgtcgc
     6661 atctgcctgt cactggatga aacaagccgg agaacgcgca gtcgccagaa gcgaggggcc
     6721 aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc
     6781 atgaaacgaa ttaacgctct gacgattgcc ggtactgatc cgagtggtgg tgcggggatt
     6841 caggccgatc ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca
     6901 ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc gcattgagcc tgattttgtc
     6961 gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg acaccactaa aatcggtatg
     7021 ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa
     7081 aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct ttcaccttcg
     7141 gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg
     7201 cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa
     7261 caagggcgat cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg
     7321 gatgatgagc aaagcccgga ctggctgttt acccgcgagg gtgaacaacg gtttaccgca
     7381 ccgcgcatta tgaccaaaaa cacccacggc actggttgta cgctctctgc ggcgttggct
     7441 gcactacgcc cgcgccatac aaactgggct gacaccgtac aggaggcaaa aagctggctt
     7501 tcatcagcat tagcccaggc cgacacgctg gaagttggtc acggtattgg tccggttcac
     7561 cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg
     7621 gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac
     7681 cgctctatta tgcgctcttg ggctaatttc catcgttgca atttatccgc gtcagacggt
     7741 gaattttttc tattcgacag cagttcagat tacagactac atccatttct acggttatcg
     7801 cccggttaaa tctttcgcta ttcgcattcc tgccagttac accattcacg gaatagatgt
     7861 ttctcgctgg caggagcgga tcgactggca gcgggtggca aaaatgcgcg acaacggtat
     7921 ccgcttacag tttgctttta ttaaggcgac ggaaggcgaa aagctggtgg acccgtattt
     7981 ttcgcgtaac tggcaactaa gccgcgaaaa tggcctgctg cgcggggcgt atcattattt
     8041 ctccccgtcg gtagcagcgc cggttcaggc acgattattt ctgcaaacgg tggatttctc
     8101 acaaggcgat ttccctgcgg tactggacgt agaagaacgg ggaaaattat cggcaaaaga
     8161 attacgcaaa cgagtaagtc agtggctaaa aatggtcgaa aaaagtacgg gaaaaaagcc
     8221 gattatttac tcaggagccg ttttttatca caccaatctg gcgggctatt tcaatgaata
     8281 tccgtggtgg gtggctcact attatcaacg tcgtccggac aatgacggca tggcctggcg
     8341 cttctggcag cattccgacc gtggacaggt agatggcatc aatggtccgg tggattttaa
     8401 tgtatttaat ggcacggtgg aggagttgca ggcattcgtt gatgggatta aagaaacgcc
     8461 ttaaataaga agcagtgata tactcccacc agactccccg gcaggaaaaa gacaaatgga
     8521 acaagcgcat acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac
     8581 gccgctttat attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga
     8641 gcatggcaat attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat
     8701 tacggtgcgc aaggcgatgc aggcgctgga agaagagggt gtggtgacgc gttcgcgcgg
     8761 ttacggcacg cagatcaaca acatcttcga atactcgttg aaagaagcgc gaggtttttc
     8821 tcagcaggtg gtattgcgcg ggaaaaagcc cgatacgcta tgggttaaca agcgtgtcgt
     8881 gaaatgtccc gaagaagtcg cggagcagct ggcggtcgaa gcaggaagtg atgtcttttt
     8941 gcttaagcgt attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc
     9001 ggcgcattta attcatgatg ttgatgccat cgggatttca ctttatgact atttccgcag
     9061 ccagcacatt tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccga
     9121 gtttcagtca catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc
     9181 gcttgaccaa cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata
     9241 cgtttttgtg tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc
     9301 cgacaaccca cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag
     9361 aggccagccc ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa
     9421 cttgcgttgg aaatgccgga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt
     9481 ctttatcgag acgaacgatc aacggcgcgg caaatttctc ctgccattgc tcgccaagtg
     9541 ttgttatctc tgcggataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga
     9601 gcgacactaa aggtcgacag gccatgatcc gcgccagtaa tgcatccggg atatcgccaa
     9661 tacgtgggcc aaaatcgata aacggcgtca cgtcttgcag ctcttccagc cattccacta
     9721 acaattcgcc gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg
     9781 caacggttaa tcgcgccagc cactggcgat tccactgatt ttccacaccg ctgaatgaca
     9841 taaaagtacg ttcgccatcc ggctcaacca gcgccagaca ccagccgtta tcgccttcgg
     9901 cgttatcgat caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc
     9961 ataccccctg accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca
    10021 cggcaatatt cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc
    10081 acccacgcca ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga
    10141 ccatcaccga ctgacgcgtg gttaattcag gcagcagcgt gtgtaatcga gcgccgctca
    10201 taccgcctcc cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag
    10261 ctgatttacc gcatccagct ccgcctttaa cgcaggatcg atagcgttaa tgccatgcaa
    10321 cgcgccgcag attgccgtcg ccatagcacc gatggtgtct gtatcgccgc caaggttagc
    10381 gcagaggacg gcgcaacggt tcgggtcggt ttgtgccagt tcaaccagcg caatggcgca
    10441 cggaacggac tcaatagtgc tggtacctgc gccaacgacc tggtaaagct gttcgctggc
    10501 ggattcggtg ccgtcggcat tgcgcacaat tttcagcgca atctccagcc gcgccgccag
    10561 tgaggcgctg aacgtggtgg tgcgtttttg ctgggcatgt cgcgcaattg aaggcagtga
    10621 atcaacaatc gctgaccagc tttctccgtc aatagcacga gaaatcgccc atgcgatgac
    10681 taccgcacct gcaactgcca gatcggattt atgggtcggg ctcgacgcca gcgccacatc
    10741 atcaataaag gaatcaacat cgcgcgccgg aagcaaacaa cctaatggcg agacgcgcat
    10801 cgctgcgccg ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat
    10861 ggcgttaagc gcaatcttcg aggtcggacc tagcacgttt ttgttgaagg cgtcgaaacg
    10921 cagcgcccag tcgaggatat tacgcccaat cagatccgga tcgatcttgc ctttacgttc
    10981 cagcaacgca tccgccagac acagcgccat cgaggtatcg tcggtgaatt cggcgcggtt
    11041 aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa tccagccaaa
    11101 gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg catcccctaa
    11161 cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc cccttgtgtc
    11221 aacgcaatat cgcgatcatc gaccttgata gccgtaattt cgttgtcgga ttcgcggaaa
    11281 aaaatcatga acagcacggc gataatggca atcatcaccg cgccaaaagt ccacatcccg
    11341 gaccagttga aagtcagtcc gtttaccggt tcctgataag cgaacatctt ttccatcatc
    11401 acaccgccaa gacgatagcc gagcaaactg ccgaagccct ggcagcagag cgtgatcagc
    11461 ccctgcgcag cggtacgcat atgcacgggg gcttttttat cgacatagat gtaagcggta
    11521 acgtagtaaa aatcgtaact tacgccgtga agcaaaatac cgaggaacag taacgcgtag
    11581 gtgaaatatt catccgcact accgtaaata aagaagccat agcggatcgc agcggtgacc
    11641 agaccaagca ataatacctt tttgatacca aagcgtttag tgaaaaacgg caatgccagc
    11701 ataaagaaga tttcagagaa ctggccgagc gtcatccagc cggtggcgtt tttcatgcca
    11761 acttcggtca gataaccgtt ggcaaagatg taatagaacg ccaatggcat cgcaaacagg
    11821 aatgaacaga agaaaaagac gaggaagttt ttatcgcgca gcaggatcag cgcatccagg
    11881 ccgagcatga ctttaatatc cattttgccg gtgctttttg gtggcgtgtc gggcaggaaa
    11941 aacgcaaaca caccgagcag agcagaactt ccggcggtaa tcagcagcgg gatgttagtc
    12001 ggtgagatat cggcatatcc cagtatttgc ggcaagaaac cacatgccag accggaggca
    12061 atccagccga tagtgcccat cacacgaatg cgcgggaaat cacgctccac atccggcacg
    12121 ttggcaaaag cgatgctgtt agtcagcgca atggtcggca tataggttag agagtaagcc
    12181 agcagtaacg gaaagaaccc ggcaaaagtg gtctgttgcg cagcgaaata catcagcagc
    12241 gcgcctgcga acatcaatac cgccagcact ttttgcgccg agaaaaagcg gtcagtgatg
    12301 gagccaacca gaattggcga caggatcgcc gcaatggcgg tacaggcata cgaccagcca
    12361 atttctccgg cactaaaacc gcttttactt aaccacaacc acaatggcac aaaccacgcg
    12421 ccccagataa accattcaac aaacatcatg aacgacagct ttgctgttgt tttcatcttt
    12481 taattccttc atgacaggta atgtaaggta cgcgatgaca ataccactta ataatacctt
    12541 ttaaatacct ttgcggtgaa aacttgaccg cgctaacatt ttttctgatg aatcgagcca
    12601 aaagaaaacg ctgaaaaaac atccaaaaga tggaaaaact cgcccgtaac gaccaggatt
    12661 atgattgccc gcaaaaggcg ggccaggaca attcatagcc gatatccaat cggaatttac
    12721 gggagcatag taatgacaga tattgcgcag ttgcttggca aagacgccga caacctttta
    12781 cagcaccgtt gtatgacaat tccttctgac cagctttatc tccccggaca tgactacgta
    12841 gaccgcgtaa tgattgacaa taatcgcccg ccagcggtgt tacgtaatat gcagacgttg
    12901 tacaacaccg ggcgtctggc tggcacagga tatctttcta ttctgccggt tgaccagggc
    12961 gttgagcact ctgccggagc ttcatttgct gctaacccgc tctactttga cccgaaaaac
    13021 attgttgaac tggcgatcga agcgggctgt aactgtgtgg catcaactta cggcgtgttg
    13081 gcgtcggtat cgcggcgcta tgcgcatcgc attccatttc tcgtcaaact taatcacaac
    13141 gagacgctaa gttacccgaa tacctacgat caaacgctgt atgccagcgt ggagcaggct
    13201 ttcaacatgg gcgcggtggc ggttggtgcg actatctatt ttggctcgga agagtcacgt
    13261 cgccagattg aagaaatttc tgcggctttt gaacgtgcgc atgagctggg catggtgaca
    13321 gtgctgtggg cctatttgcg taactccgcc tttaagaaag atggcgttga ttaccatgtt
    13381 tccgccgacc tgaccggtca ggcaaaccat ctggcggtaa ccatcggcgc agatatcgtc
    13441 aaacaaaaaa tggcggaaaa taacggcggc tataaagcga ttaattacgg ttataccgac
    13501 gatcgcgtgt acagcaagtt aaccagcgaa aacccgattg atctggtgcg ttatcagtta
    13561 gctaactgct atatgggccg ggctgggttg ataaactccg gcggtgctgc aggcggtgaa
    13621 actgacctca gtgatgcagt gcgtactgcg gttatcaaca aacgcgctgg cggaatgggg
    13681 ctgattcttg gacgtaaggc gttcaagaaa tcgatggctg acggcgtgaa actgattaac
    13741 gccgtgcagg acgtttatct cgatagcaaa attactattg cctgatacgc ctcatacgaa
    13801 cagcaccacc ccgtaggacg gataaggcgt ttacactgca tccggcaacc tatgcctgat
    13861 gcgacgcgga agcgtcttat catgcctaca tagcactgcc acgtatgttt acaccgcatc
    13921 cggcataaaa acacgcgcac tttgctatgg cttccctatc gggaggccgt ttttttgcct
    13981 ttcactcctc gaacaatttt catattgtcg ttttgtgatc
//
LOCUS       DAPHJE010000110        13853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPHJE010000110 DAPHJE010000000
VERSION     DAPHJE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.111"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..605
                     /locus_tag="RH222_003491"
     CDS             39..605
                     /locus_tag="RH222_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3798454.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            656..3115
                     /locus_tag="RH222_003492"
     CDS             656..3115
                     /locus_tag="RH222_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3798455.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSEQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3130..3861
                     /locus_tag="RH222_003493"
     CDS             3130..3861
                     /locus_tag="RH222_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3798456.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3858..4919
                     /locus_tag="RH222_003494"
     CDS             3858..4919
                     /locus_tag="RH222_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3798457.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5071..6117
                     /locus_tag="RH222_003495"
     CDS             5071..6117
                     /locus_tag="RH222_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV3798458.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6114..6905)
                     /gene="nei"
                     /locus_tag="RH222_003496"
     CDS             complement(6114..6905)
                     /gene="nei"
                     /locus_tag="RH222_003496"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV3798459.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="RH222_003497"
     CDS             complement(6941..7675)
                     /gene="pxpA"
                     /locus_tag="RH222_003497"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV3798460.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="RH222_003498"
     CDS             complement(7665..8597)
                     /gene="pxpC"
                     /locus_tag="RH222_003498"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV3798461.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="RH222_003499"
     CDS             complement(8591..9247)
                     /gene="pxpB"
                     /locus_tag="RH222_003499"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV3798462.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="RH222_003500"
     CDS             complement(9270..10013)
                     /gene="ybgI"
                     /locus_tag="RH222_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV3798463.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10284..11765
                     /gene="dtpD"
                     /locus_tag="RH222_003501"
     CDS             10284..11765
                     /gene="dtpD"
                     /locus_tag="RH222_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV3798464.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVQMIWLYQALKFRNRALALES"
     gene            complement(11807..13225)
                     /gene="phrB"
                     /locus_tag="RH222_003502"
     CDS             complement(11807..13225)
                     /gene="phrB"
                     /locus_tag="RH222_003502"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV3798465.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13222..13731)
                     /locus_tag="RH222_003503"
     CDS             complement(13222..13731)
                     /locus_tag="RH222_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV3798466.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         3327 a         3723 c         3411 g         3392 t
ORIGIN      
        1 ccgatatact tttttctttt ctgttaaagg actccgttat gtttaaagga caaaaaacat
       61 tggccgcact ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat
      121 ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg
      181 atattgataa agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc
      241 acagcgatgc cgttgctgtc gacctgcgct tagtcaactg tgatctggaa aactccagca
      301 acggttccgg tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca
      361 ccggcgcaga tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac
      421 gtttaatgaa taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc
      481 tggctccgac ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc
      541 aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata
      601 aataagtatt actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga
      661 caccgtgaat atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc
      721 attggcggtc gaattcaacc ttaatgttct cgataaatca atgcgcgacc gcattgatat
      781 ttcattatta aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt
      841 aaataataac caaatcagta acgggcaaaa gattaactgg cacaaaaatg acgataaaac
      901 cattccgtgc atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca
      961 gtcgttacca ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa
     1021 tttcgatcaa gccaatcagc aactaaatat caccattccg caagcctggc tggcgtggca
     1081 ctcagaaaac tggaccccac cctccacatg gaaagaaggt gtcgccggtg tcctgatgga
     1141 ttacaacttg tttgccagca gctaccgccc acaggatggc agcagcagca ctaacctgaa
     1201 cgcctacggt accaccggaa ttaacgccgg ggcatggcgc ttacgtagtg attatcagtt
     1261 gaatcagact gatagcgatg ataaccatga acagtcaggc ggaatatcgc gcacctatct
     1321 ttttcgtcca ttaccgcaat taggctctaa attaaccctc ggcgaaacgg attttagttc
     1381 caatattttc gacggttttt cttataccgg cgcggcactg gcaagtgacg agcgaatgtt
     1441 gccatgggaa ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac
     1501 ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt
     1561 tatcattgac gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga
     1621 agatggtcgg gtgaacaatt tccaggtttc ggcagcatcg acgcccttcc tgactcgtca
     1681 gggacaggtt cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac
     1741 tgaaaatgaa acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct
     1801 gtacggcggc ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtattgggca
     1861 aaatatgctg tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga
     1921 tactcagcag gacgagcggg gcttaagcta ccgttttaat tacagcgaac aagtggatgc
     1981 caccaacagc acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta
     2041 cgccaactat cttgatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat
     2101 cagcttatct gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct
     2161 acatcaaacc tggtggaatg cagacgcctc cacgaccgcc aacatcacgg caggttttaa
     2221 tgttgatatt ggtgactgga gagatatctc tatttcgacg tcattcaata caacccatta
     2281 cgaagataaa gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg
     2341 tggtcgggtt ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg
     2401 gaacgatacg ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg
     2461 tccggacaat ggagcccagg tgagcggtaa ctatcagcat ctgagttcag cgggtgagtg
     2521 ggatatttct ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg
     2581 ttctttcacc gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc
     2641 acgcctgatg gtcagcaccg atggcgtggc agatattccg gtccagggca atctcgacta
     2701 caccaaccat tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt
     2761 ggcggtgaac atgaatgact tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga
     2821 aacgtggatt gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt
     2881 taacgtcatc attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca
     2941 ggatgacagc ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg
     3001 agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat agccagcatt gctcgctcca
     3061 tcttcctgaa catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag
     3121 gaaatgaaaa tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc
     3181 agccacgctg cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct
     3241 accagtctgc gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc
     3301 gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt
     3361 ctggaaccga aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta
     3421 ccgggcgatc gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa
     3481 aaaaacagcg accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg
     3541 cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc
     3601 cagcagggca accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac
     3661 ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt
     3721 agtacggtca acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg
     3781 gatgattacg gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa
     3841 gccgtggagg cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat
     3901 ttctgccgtt gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg
     3961 tagaaaaatc agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata
     4021 agatttggga atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca
     4081 attttgaaag tgagcatgtt tatgcctggg taaaccccta cccgggggtg caggatcgct
     4141 attatcaact cggcgtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc
     4201 gcatcgacac taaccagtgt attgacagta agaacattga catttatacc cctgagcaga
     4261 ttatcgcgat gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt
     4321 cgcgtacctt tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg
     4381 attatcaaag tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta
     4441 acgaagatcc tactgcccaa aacctgaaat atcatattac cggtctggaa aacattcgcg
     4501 tgctggattg cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta
     4561 aatttaattt gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga
     4621 caaaatcaca aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg
     4681 aagaaacgtt ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt
     4741 tgctggatga aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca
     4801 ccagcgacat gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta
     4861 cccccatcga ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat
     4921 ttgccatgat gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc
     4981 ccttttcctt gattgctgtt attttttcct ggcccggctt tctttatgct gtatcgatac
     5041 accaaagaga aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt
     5101 gtgctatcac ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta
     5161 cttgggccga tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc
     5221 cgctccgctt ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact
     5281 ggttcgattc tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg
     5341 acgctgcttt ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga
     5401 aataccggtg cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa
     5461 gattacggcg cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc
     5521 gcgggggccg ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac
     5581 cagcaaatcg tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca
     5641 gtcgttgccg gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg
     5701 atgctggcgg gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg
     5761 ctgctggcga tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa
     5821 atcttactgc gggcactacg cccactaccg caaatcctgc tgtcgatttt tgctctgctg
     5881 gctatttgtg caggtatggc gtggggactg acccggttta tgcatattga ttttatgact
     5941 gcctacctcg ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc
     6001 aatgccgata tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg
     6061 acggggccag ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc
     6121 tggcagcctg gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg
     6181 ccacagcgtt cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca
     6241 tgatgcttat tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt
     6301 aacgcgtgtg cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca
     6361 gtcaacccaa cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac
     6421 gcctgatcga gcagtaatcc tgcaaactga cggttacgaa agcgcggtga caataaccgt
     6481 tcctttacca cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa
     6541 aacggatgcg tggtcagttg ttccggggtc aacatctcaa tatccgaggc gctataaagc
     6601 agaatggttt tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct
     6661 tcgccggtat caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaag
     6721 tcgttggaaa aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga
     6781 ccgataagtt gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt
     6841 ggtttgcctt tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca
     6901 ggcatggtga tatccttttt tcattcttta ttaagcgggg ttatgctgcg acaacaatcc
     6961 cctttttggc aaatgtagct cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc
     7021 catgcaggca gaccgtttgc gccgcgaccg ttgcccattc gccggtgata cttttgactc
     7081 tgccgtgttg caccatctcc agcgtttgcg ccagcgcctg ttgttcgttt tcaatcaacg
     7141 cgcctcgctg acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca
     7201 cttcctcgcg cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca
     7261 gcccaacgag aatcaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca
     7321 gttgtgcttc tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta
     7381 cgccgccttg cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt
     7441 aaacggtttc tggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac
     7501 tcgggtgagc gccaattgca acaccatttt ttattgcttc acgcacgcaa gcatgcatgg
     7561 tttgcgcatc gcctgcatga aatccacagg caatattggc agaggaaacc agcgttaata
     7621 gctctgcgtc gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc
     7681 agccgccacg ctaattgttc aaaataacgt tgctgatctt gccgcgcttt cagtgcctct
     7741 tccagtgaac actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac
     7801 atatcagcct caatgatgca ggcaatacgc gggtaaccac cggtggtctg tgcgtcgttc
     7861 atcaacacaa tcggctgccc gttatgtggc acctgcacca cgcccggtaa caaaccgtga
     7921 gataacagtt cgcgatcggt ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatg
     7981 cggttacttt gcgggctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga
     8041 tcgaactcat gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt
     8101 ttaacgccct gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca
     8161 tccttcagta aacggccttc cagcccgcca atccccacgt tgagatcggt gctgcatgac
     8221 cccattaccg gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc
     8281 tgcgggcgtt taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag
     8341 acggcattat catccagccg cgcttcgcaa ccggcacccg tcagagcaaa ccacccatca
     8401 gtttcgaact caacagttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca
     8461 ttacccacca gtaggttagc aatgcgtaac gcgggcatat ccagtgcgcc gcagtggctg
     8521 atacccgact ggcgaaaacc gtgacgaccg ccatcctgca cagtggtata catgcccgca
     8581 cgaataatct tcagcatact ccctccttct gcggtacaaa gcgcacgctg tctcccggac
     8641 gtaataagat gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca
     8701 actgccagcc acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga
     8761 cagaacctgc cggaacgagt aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg
     8821 gcaaactccc gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca
     8881 cggaggagtg caattcaaca acctgttttt cgctcaaccc gcaatgcgcc gcgaccaccg
     8941 ccaaatccgg tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag
     9001 gttccagggc ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca
     9061 gcgactcagg attacgcaaa atcaccgtaa tattgttcat gccgggaatg gcttcaacca
     9121 cattcggcat atccaccaga cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg
     9181 tcaccggcgg ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc
     9241 gttgcacttt cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc
     9301 acatcaagat cggtattttc attcagccac tcgctcaatg cgcgaatacc accacgttca
     9361 gtggcatggt gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc
     9421 tgttcagaaa cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata
     9481 aaactttgcc cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg
     9541 ccacaccata acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccagtccc
     9601 ggcacaggca tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg
     9661 atccccagta acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc
     9721 aaatgccagc catacaggtt gatatcattc gccagcaacg tttttaaacg gttacgcttc
     9781 attccgcgaa tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca
     9841 tccgcgccca gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca
     9901 attttttgca ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc
     9961 gccgcgctgt tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg
    10021 ccttccgtat tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag
    10081 ccaatctttt gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc
    10141 atcacgcttc agctaatgta tatttatgca atcagacagc atattgcata aataatcaga
    10201 tctgtatcaa agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg
    10261 ttgccgtcaa ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact
    10321 atgtcgttgc gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga
    10381 ttctctatct caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg
    10441 cctactgttc gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc
    10501 tcggcaatcg catggcggtg atgctggggg cgttgttgat ggcgattggt catgtggtgc
    10561 tgggtgccag tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg
    10621 gctatggcct gtttaaatct aacgtaagct gtctgctcgg cgagctgtat gagccaaccg
    10681 atccgcgtcg tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta
    10741 tcgcacctat cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc
    10801 tggcggcggt tggcatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca
    10861 ctcatacccg cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact
    10921 ggggatggct gctggttctg ctggtggcaa cgcctgcact gattaccgta ctgttctgga
    10981 aagagtggtc ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa
    11041 aaatttatcg caaagcagaa aaccagaaac agcggaagga gctggggctg attgtgacgc
    11101 tcaccttctt cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc
    11161 tttatatcga ccgcttcgtt aaccgcgata tgtttggtta taccgttccg accgcgatgt
    11221 tccagtcgat taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtaa
    11281 aagagagtgt cgcgggtaat cgtaccgtgc gcatctgggg gaaatttgct cttggccttg
    11341 gcctgatgag cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg
    11401 gtcactcttc tctgccactg atggtattag gcctggcggt gatgggcttt gcggaactgt
    11461 ttatcgaccc ggttgccatg tcgcaaatta cgcgcattga aatccccggt gtgaccggcg
    11521 tattaaccgg catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga
    11581 ttgccgatca gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca
    11641 atgcatatat tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg
    11701 tacagatgat ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggaat
    11761 cttaataggc cagcaccata cctgataaaa cgctcgctgc gttttatcag gctcctttcc
    11821 tcgcttcttc gtacgctgcc agcgttcgca gccttgcctc tttgtgatca actatcggtt
    11881 gcggataatc cagcatcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt
    11941 tccctggtac atcgcgcagc tccggtaacc atcggcggat aaactcacct tcgcgatcaa
    12001 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcagtacccg
    12061 tagacgccgc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
    12121 acatgaaata tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg
    12181 ctgaaatcat ccgtagccgg ttatgcatcc agccagtgct gttaagctga cgcatggcgg
    12241 catcgacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
    12301 tctgccactg cacacgatcc gtccaggcaa taaacggaca atgtttacac aacgaggggt
    12361 aatacgtcat cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac
    12421 cggccccacc gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag
    12481 gcgataaccc gcccgttgcc aggctggcgg acaaacgact ggtgccttcc actgccggaa
    12541 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
    12601 tcgccgcttt ttcttccacc gggaaatgcg cagtatcgaa agactgacga ggataattca
    12661 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
    12721 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
    12781 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
    12841 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
    12901 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
    12961 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
    13021 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
    13081 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
    13141 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcctggc
    13201 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
    13261 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca ggataaagcg ccatatagtg
    13321 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
    13381 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
    13441 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
    13501 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
    13561 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
    13621 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
    13681 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
    13741 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
    13801 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagcga gaa
//
LOCUS       DAPHJE010000111        13691 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPHJE010000111 DAPHJE010000000
VERSION     DAPHJE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.112"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..546
                     /locus_tag="RH222_003504"
     CDS             <1..546
                     /locus_tag="RH222_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798467.1"
                     /translation="INKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQR
                     ITTFFHTSPDVKNREIRLEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDE
                     IQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIV
                     ISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(789..1484)
                     /locus_tag="RH222_003505"
     CDS             complement(789..1484)
                     /locus_tag="RH222_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV3798468.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVILFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="RH222_003506"
     CDS             complement(1477..2904)
                     /locus_tag="RH222_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV3798469.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="RH222_003507"
     CDS             complement(2915..3634)
                     /locus_tag="RH222_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV3798470.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELFTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4162..5016)
                     /gene="rclR"
                     /locus_tag="RH222_003508"
     CDS             complement(4162..5016)
                     /gene="rclR"
                     /locus_tag="RH222_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV3798471.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5242..6567
                     /locus_tag="RH222_003509"
     CDS             5242..6567
                     /locus_tag="RH222_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV3798472.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6676..6912
                     /locus_tag="RH222_003510"
     CDS             6676..6912
                     /locus_tag="RH222_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3798473.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKTILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6924..7517
                     /gene="rclC"
                     /locus_tag="RH222_003511"
     CDS             6924..7517
                     /gene="rclC"
                     /locus_tag="RH222_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV3798474.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7677..7805)
                     /locus_tag="RH222_003512"
                     /pseudo
     CDS             complement(7677..7805)
                     /locus_tag="RH222_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            8108..8959
                     /locus_tag="RH222_003513"
     CDS             8108..8959
                     /locus_tag="RH222_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3798475.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(9099..13355)
                     /gene="fdeC"
                     /locus_tag="RH222_003514"
     CDS             complement(9099..13355)
                     /gene="fdeC"
                     /locus_tag="RH222_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDV3798476.1"
                     /translation="MSHYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLAKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVIIGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     AFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNDDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNTISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKMTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQATLEVDGSTPKVANDNDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSDVTLTASSENLVLDPKGTAKTNEQGQA
                     VFTGSTTIAATYTLTAKVEQANGQVSTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATMTVTLMAGVNPVGGSMWVDIEAPEGVTEKDYQFLPSKADHFSGGKITRTFSTSK
                     PGVYTFTFNALTYGGYEMTPVKVTINAVAAETENGEEEMP"
BASE COUNT         3568 a         3424 c         3015 g         3684 t
ORIGIN      
        1 atcaataaag acaatataaa agtgatttac agcctaaaaa taaaccagac aacgccaggt
       61 atacttgtta gctgtgatag caataataat tttgcctgtc agactacaat gactgaagat
      121 gttattcagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      181 aggctggaat ggtcaggaga taagagagct ttaccaactg ctgaagagga aatatctcgc
      241 gtgcaggcct ctattatcaa atggtatgcg tcagaatatc ataatggcag gcaagttctc
      301 gatgagatac aaacgccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 cagtaataaa caacattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaacc cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt
      661 ggtaatacca ggatatttac agaagagcag cataaccatg acggaacggg gttaagtcag
      721 gttgtcctaa acgctatttt caacctcgta tgccttcttc aggtttatgt ccagacttca
      781 tatctctctc aacaatcctc aataatcaga tacaccgctt tcaccgggcc atgaactccg
      841 acgactttga taagctcaat atccgccgtt gaactggggc cgctaatgat gttaatgcag
      901 gaaggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcagga tagtgctttt acgcaggata aaaagagaat attccgggag caggctcaat
     1021 gaacgcccgc gctcggcggc ggaaaaaaga atcacgcctc ccgattcggt taaaccatat
     1081 tcagcataca caacacccac tttagcctgc tctgcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcgctaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcaccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgttgtc
     1801 acaagctgtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gcccagcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac caggcatacc gaaccggtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tatattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt
     2641 tcttgcaaaa tagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg tttagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc gcaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtaaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacaccg
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttg cggcacgtga tgcccattca ggttcatccg
     3361 ccagatacat cgggtaactt tttacggcat aggtgcagga gccagccggt gaaataatgg
     3421 gatcatcgtt atcctccagc gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcaaaactaa tactccatta cttcatgccc
     3661 atttatgccc gatgtttccc ccccaccggg ggatcaatca tcaataaata tggcgttctt
     3721 ttttaattgg ctagcaacat accacccctc atttgtatga caaatgataa tctttcatcc
     3781 aaacgtcaaa tgcaataact aattttattt ctttcggcat cgctcacatt ttattgggaa
     3841 atcgacgatc atttttggca taagaaatcc attttaaaat ggctacatcc cttataccta
     3901 ttcagagcta cttattcagg ggttattgag atatacactg atggtgtaca aaaaaatcac
     3961 cgacaataat cacgtatcaa gtaatagtcg ggattttatt taattgaagc aatttagttt
     4021 gcatttttat ttaaaaactg agctagagtc catcagaaaa aaatatataa agcccaaacc
     4081 accgttggaa atagaaatag ttgaaaggct cacaactccc ggaatgcgga ttatctttgc
     4141 cctgatttct ggcggtttta ttcagggtgc aagctgtctg accctttccc gatattctcc
     4201 cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc
     4261 tactgactca gcgatcacca caacagggag catttcccgg gaaaacatct gggccgctat
     4321 ttgtagacgc aactttgtta atacagccag cggcgtggtt ccggaaacat cacggaaaag
     4381 ctgggcaaaa cttgcccggg acatgtgggc gatgctggcc agcgattcga cggtccaggc
     4441 gtgtcctggc atttccaaca tttgctgtat taccgcacca agacgtggat gcagaagcaa
     4501 actgagaatg tttttctcag tattaacctg tgcaatccac tcacgcaccg cgagggtaaa
     4561 gaatgtagcg cagatctggc tacacagtgc atccactccc ggcattgcca atctggattc
     4621 ctgttgtaaa aacgggatcg cctccctcag ccagttatat tctacgctgt ggttaaccgg
     4681 tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa cgcgccgaat gttgcagccg
     4741 aagagtgccg cagacaatac aggttgattc attatcgaca tgactcaggc gatgggctga
     4801 attttgtggt agcaggacca catttcccgg gcgtaatgta aaaatctccc ccgtcggcat
     4861 ttccagcttc gccgctcctt gcgttaacgc atgccaacga ataaccgata attccccggc
     4921 accatgtgga agctgccagt cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg
     4981 agcgttaagc atcaacaaac ggctaagggc atccatatca gactcctgag tggtttttga
     5041 ttacaaacac caacaatctt agtaaataac taatctaata ccatcaaata gttaatcatg
     5101 atttttgttt tgtgattctg attaagaaaa agaaaatacc agacgataag acaaaatatc
     5161 aagcgttgtg aagaaatgtt atttgctctt ttgcgtctat agtcatgatg tcaaatgaac
     5221 gcgtttcgac aggaaatcat catgaataaa tatcaggcag tgattattgg ttttggcaag
     5281 gctggaaaaa cattagccgt cacgctggca aaagcaggtt ggcgagtggc tctcatcgaa
     5341 caatcaaatg caatgtatgg cgggacctgt attaatatcg gctgcatccc aaccaaaaca
     5401 ttggttcatg acgcacagca gcacacagat tttgtccgtg ccatacagcg taaaaatgaa
     5461 gtggttaatt ttttacgtaa taagaatttt cataatcttg cggatatgcc caatatcgac
     5521 gtgatcgacg gccaggcgga gtttatcaat aatcatagcc tgcgtgttca tcggcctgag
     5581 ggaaatctgg aaattcatgg cgagaaaatt tttattaata ccggtgcaca aaccgtggtt
     5641 ccgccaattc ctggaattac caccacgcca ggagtatatg acagcaccgg attacttaat
     5701 ctaaaagaat tgcctgggca tttaggtatt ttgggcggcg gatatattgg cgttgagttc
     5761 gcctctatgt tcgctaattt tggcagcaag gtaaccattt tagaagcagc ttcactgttt
     5821 ttgcctcggg aagaacggga tattgctgat aatatcgcga cgattttacg cgatcagggc
     5881 gtcgatatta tcctcaatgc ccatgtggag cgaatcagtc accatgaaaa tcaagtgcaa
     5941 gtgcatagcg agcacgccca actggcggtg gatgcactgt taatagcttc cggtcgtcaa
     6001 ccggctaccg cttcgttaca tccagaaaat gccggtatcg cagtaaacga gcgcggggca
     6061 attgtcgttg acaagcgatt acataccacc gcagacaata tttgggcgat gggagatgtt
     6121 accggcgggc tgcaatttac ttacatatca ctggatgatt accgcattgt acgtgatgag
     6181 ttactgggtg aaggcaaacg tagtactgat gatcggaaaa atgtgcctta ttccgtattt
     6241 atgacaccgc ccctgtccag ggttggtatg acagaagaac aagccagaga gagtggtgct
     6301 gatattcagg tggtgacatt gcctgtagct gcaattccgc gcgccagagt gatgaacgat
     6361 acccgtgggg tattaaaagc gattgttgat aataaaaccc aacgtatatt aggggcatca
     6421 ctgctgtgtg ttgactccca cgagatgatt aatatagtga aaatggtgat ggatgccggg
     6481 ctgccttata gcatattacg cgatcagata tttactcatc cgtcgatgag cgaatcactc
     6541 aatgatctat tttcattagt caaataaact caaaatcaga cgccagaaca aatattctgg
     6601 cgtctcagag aaaagaatct tattaattcc ctgttactct ataacttctt aatcacttca
     6661 ttgatggtat tttatatgtt taaaaaatcc gttttatttg caacactatt atctggcgtt
     6721 atggcatttt ccaccaatgc agatgataaa acaattctga aacatatcag cgtctcgtca
     6781 gtatcagcat caccgacagt tctggaggat gccattgctg atatagccag aaaatataat
     6841 gcttcatcct ggaaagtcac atcgatgcga attgataata attcaaccgc aacagcagta
     6901 ttgtataaat aaggatgttc acaatggaaa aatacctgca cctgttaagt cggggagata
     6961 aaattggcct gacattgatt cgtctgagta ttgcaattgt ttttatgtgg attgggttat
     7021 taaagtttgt cccttacgag gcagacagca ttacaccatt cgtcgcaaac agtccactaa
     7081 tgtcgttctt ttatgaacac ccggaagact ataaacagta tctgactcac gaaggcgaat
     7141 acaaaccaga agcaagggca tggcaatcgg ccaataatac ctatggtttt tccaacggtc
     7201 ttggcgtcgt ggaggtgatt attgctctgc tggttttggc taatcctgtc aatcgctggt
     7261 taggtttatt gggagggctg atggcattta ccacaccgtt ggtaacactc tcatttttaa
     7321 tcaccacccc ggaggcatgg gtacccgcat tgggtgacgc tcatcatggt ttcccttatt
     7381 tatccggtgc tggtcgcctg gtattgaaag atactctgat gctggcaggt gcagtcatga
     7441 taatggcaga ttcggcgcgg gaaattctta aacaacgcag taatgaatcc agttcaacgt
     7501 taaaaactga atattgataa tccgaacact gtctgttgtt cactttttca ggcgtcaggc
     7561 cccctttctg atgatgaagc ctgacgccat attaccggag atatgacaga gtgaagatca
     7621 atatcactcc cctgttaaaa ggtttttgtc gtcttaggta aaatacatga ttaatatcaa
     7681 tcatgtattt ttacagcaag acaaccacga acaaattcag gattaaaatg ttttaccgac
     7741 tccccaacat aacccaaatc aagcgaacta atttgtgcca tttctttatc tgtcagtgag
     7801 aaatcgattt ttgatgggca tctgcaatac tctgaagcag actatacgga aaattccact
     7861 gtcagtccct ccattaggca tgaacaatga gtctacgtta aaacgtaacc tcaaagtagt
     7921 atgtggattt tgatatcact tatgcaaaaa attcattaat aatgtaggac tgaaacctct
     7981 ctattttcgg ggacaacgaa gcagacgcta ccagtgcttt tgccttcgcc cttgctattt
     8041 ttgatacact tagggcccag ggtataacga aaatgtgcga tatgacaaag aattaacgga
     8101 gaatgagatg atcaggcaga agattctaca gcagctcctg gagtggattg agtgcaatct
     8161 tgagcaccct atttcaatcg aagatatcgc acagaaatct ggctacagca gacgcaacat
     8221 ccagcttctg ttccgaaatt tcatgcatgt gcctttggga gaatacattc gcaaacgaag
     8281 gctttgtcgt gccgccattc ttgtccggct caccgcgaaa tctatgcttg atattgcact
     8341 ctctttgcat tttgattcac agcagtcatt cagccgcgaa tttaaaaagt tattcggctg
     8401 ctctccccgt gaataccgcc accgtgatta ttgggatctc gcaaatatct tcccttcttt
     8461 tttaatacgt caacagcaaa agacggagtg tagattaatc aactttcctg agacacctat
     8521 ttttggcaac tcatttaaat atgacattga agtgtcgaat aaatcaccgg atgaagaagt
     8581 caaactacga cgtcatcatt tagccagatg tatgaagaat tttaagacgg atatctattt
     8641 cgtttccacg tttgaaccgt caacaaaatc ggtcgatttg ctcacggttg aaacttttgc
     8701 tggtacggta tgtgaatatg ctgacatgcc aaaagagtgg acaacgaccc gaggacttta
     8761 tgcctctttc cgttatgaag gaaactggga aaattatcct gactgggtgc gtaacatcta
     8821 tctgatagag ttacctgcca gggggttagc cagagtgaac ggcagcgata ttgagcgctt
     8881 ttattacaat gaagatttcg tagaaaagga tggcaatgat gttgtttgcg aaatttttat
     8941 tcccgttcgt ccggtttagt tggtcactat ctcttattga gttttatcct tgcctgatac
     9001 tatttaatca gtatcaggca aggtattcag tgaataatgg cgttgaatat ttcaacacca
     9061 ttattcttta ttagatcgta actttcatac tattcaaatt atggcatctc ctcctcgcca
     9121 ttttcagtct ctgcagcaac ggcgttaatt gtcaccttca caggcgtcat ttcgtacccg
     9181 ccatacgtca gtgcgttgaa tgtgaacgta tagacacctg gcttgctggt actaaatgta
     9241 cgcgtgattt tcccacctga gaaatggtca gccttcgacg gcaggaattg ataatccttc
     9301 tccgtcactc cttccggagc ctcaatgtcg acccacatac ttcctcctac gggattgact
     9361 cccgccatca gggtaaccgt cattgttgca gtagtcttcc cgtccgccac cagagaatct
     9421 acacgttctg gagatgcggc gagcaccgcg tttttatcat ccgcgacgaa tttagattca
     9481 gcagttttcg tcgatacctg accgttggcc tgttccactt tcgccgtgag tgtatatgtc
     9541 gctgcgatag tggtagagcc ggtgaaaaca gcctgtcctt gctcattagt tttcgccgtc
     9601 cctttaggat ccagaactaa attttccgag ctggcagtca gcgtcacgtc gctatccttc
     9661 aacaggttgt tattggcatc agtcaccgta actttatacg tctgtttggt tttgccatcc
     9721 gccactgcac ggttgccaat cacctcaata ctggagatgg tcgccgtagt cttatcggcc
     9781 acaaacgtta cagactgcgc attcgaaggc tggtcatttc ctgccgacgc cgtgatctca
     9841 tacgttccgg cagtcacgga gaccactttc agttccgctt taccctcctt gtcggcgttc
     9901 accatgatat taccgtctgc aagcggtttg acgccccgag gcagattaaa gacgaccaca
     9961 gcgccaggca gaaggttgcc gtattgatcc ttaaccgttg ccgtcagcgt aaaggcatca
    10021 ttgtcgtttg ccacttttgg cgtgctgcca tcaacctcca gggtcgcctg accggtactg
    10081 aaatccgcct tgaatttcac cgtaaccgtc ttctgagcat tattcactga ggccgtgatg
    10141 ctgtgttccc ccgcaaccgt tgacataagc tcaatgtcca ctttccctgc cgcattggtt
    10201 gttactgtat tccccgtctt gctggtcaca ccctgcggta aagtcagcgt aacttcttgc
    10261 ccctgcaacg ggttaccata gctgtccacg acggtcaggg ttatctggtt agcagactgt
    10321 ccattagcca gttggttatt aaccttcact gtcatatcac gaatctcagc cttagatgcg
    10381 tcacctgcaa cgttcagcac cagtggctga gcaacggcat tttggccgtt cactcgcggc
    10441 atcacagaca actgacccgc ggcagatccc agcgttaagg tcgccacgta gaccccatta
    10501 cctttctctg tccagtttcc tgctgaagga cgctcactcc ccgtgctggc ggcaccgcta
    10561 aacactggtg catctggctt cagcccggtg accgggttcc cgtacgcatc cttcacggtg
    10621 aaggtgagtt ccgtcgtcat tttgacggtc ggagccttat tgtccgcaac aagcgtagag
    10681 ttcgctgatg acatctctcc ggcgatgacc gtcaactgcg cggcttcggt ggctgctggc
    10741 tggccgttga agagcggcat gacacgaagc tcgcccgtct ttccgcctgt agttaacgta
    10801 gcaacatagg aaccgtcacc tttttcggtc caactggaaa cggtcgcccc ttcagaggcg
    10861 gtccccgtca aacttgccga caacgaaaga ccactgatag tgttgccatg tgcatctttt
    10921 gcaataagcg tcacggttgt gctttcgccg gcttttacgt gatcttctgc gacagagact
    10981 ttcgactggt ttgaagataa cgcatcagcc accacggtta cttttgccgc atttgctgcc
    11041 gcatcctgtc catttagctt cggcataact tctaattcac ccgccgtaga gccgagagta
    11101 atctgcgcag tccacgtccc atcatcatta tttgtccagc cagatgccgt agaacctaca
    11161 gcagcggcac ccgctaatga cggcgcttcc ggcgtgaggc tggtcacagg gttgtcatag
    11221 gcatcttttg ccgtccagat tgccgtaact gtaccgccaa ccaccggttt atcaggattc
    11281 agggtgatgg acgaatgtgc tgcatcaagc ggaccggcaa caaacttcag cgtttgttgt
    11341 aatgtgccca gctgatactg ctcgctgaaa gcacgcacaa caacgtttcc ggcacgggtc
    11401 gaagaaaccg tggcgctgta aacgcctggt tttatttccg aaatggcacc aacggttaca
    11461 ccattagtgt cttgcggaac aaatcgcaag cggctggctt ctcccgtcac cggattaccc
    11521 tcggagtcca ccgcagtcaa cgtcaacgta taggcttgct gaccatcagc aaccacatca
    11581 cctgacggct cgttagcgct cagggtggag tttgccacat ccatcatcgt ggcccgcagt
    11641 tctgcagtga cggttttgct catgccatca acgctaacag taatcgttgc ctcacctgac
    11701 tgcgttccgg tagtaaagac agactgatac acccctgctt cagtttcggt gaactcaccc
    11761 agtgttggct ttgcctgtga tttagtggcc ttcagggaac gagtcacaat atttccagcc
    11821 ggtttgaagg ctagttcagt cttgatctga tctttcatgc ccgtgactgg ctgcccctcg
    11881 gcgtcgcgca gagacagcac cagcggtctt tgctcattac cgttagcaag catttgaata
    11941 cggctctgac cgtcaagcgt taacgccgtg cgatcggcgc tcatacctgc tccgataatg
    12001 accacctctg tctgcacgcg ttttgaggca ttgcctttgt tatcgtaggc aaccgcagaa
    12061 atcgcataat aattgtcttt gcctggacga taagccggga gcgttacttg ccactgacta
    12121 ccctgaccgg taattttgcc accctcagcc agtaatgacg gcgcttccca ctgcacattt
    12181 ttcagtccgt gagttgcttt gctgaccaca agccccaggg aaagtgtctg accaccctta
    12241 ccttcaatac gttcaggcag agcaatacgg atcacttcag atttgcggta ctcaagaacg
    12301 atgttgttat tacgctcaac caggtcatag cggctgcctg ccagtacccg acgctcgcga
    12361 atgctgtctg tatcgagttg tttcgccaga ggttcgccaa tccgataatt aacttccagg
    12421 ccaaagcgag tgtcattctc accactcttg ccctgcttat gcccggcgct cagtgtcaga
    12481 agaggcactg gcgtataggt cacctcggca gaaatagcat gcgggtcttt ctggcgctta
    12541 tctttaccaa acagcccgac ttcatcgcca taatactgtt catacatcag gcttgcgcca
    12601 agctgcggcc aggcgggtaa atagccctca gcacgaatat cccagccatt cgccgggcgt
    12661 tcctgataat cctcaacatc cggcgatttt ttccagccag aagcccggat ataaccattg
    12721 gcgctcagtt tcagataatc gcgccagtat tccgcaccaa caccaatgcg ggtatgacta
    12781 cgggataaat catggtcgat aaaggtgttc acccccgcca tccagtcatt tcctgaaaaa
    12841 tgacgccagc caaaaccaat atttgactga gtacgatcgt cggtacgatg tattgctccc
    12901 tgagtgaaca acatatttgt tggcgtatca taaatcggat aaagcatttc cagcgaagaa
    12961 tccttcagcg agaaatcttt atcgacattc agtttgacgc gcgcagtacc atatttcccg
    13021 agccactcct gtatttcctg gttagcttta gctgtggcca ttccggtaat aaagttacgt
    13081 gtcgcatcgc tatctggctg actgcttaaa aatgtcccgg catttgcggc aaacgacgcg
    13141 acatttttct ccacgttatt atcagcagtt accgtagtat ttcccatgct caaccgtggc
    13201 tgaaccgcat gctgcgcacg tgccgccatt actggggtaa aggtgacagc gagtggaaaa
    13261 agaacctgaa cagagatatt tgcccacgcc acgcagcggg ccagaactga ataacgaaat
    13321 cgtggttgtt tatgacctgt tttataatgt gacattgaga taatttcctg atgaacgaat
    13381 taacgaacat aggcagacac aaataatggc gtctgcttgt cagaaaaacc ggggatttcc
    13441 cgatttcctt ttccgcagaa aaggaaatac ctttatgtaa gaaagtgcac cgccacctaa
    13501 tatatccagt gcctgtagcc actcatcagg tggttgggcg cgggattata tttttattta
    13561 tgggggaaaa tttagctttt tgtgcaagtc attaaataga tacctaagtt atatttctta
    13621 ctctgcggac agtgaataat tcactattca gagtgaataa caatgacctg cctttaatta
    13681 aaactattgg a
//
LOCUS       DAPHJE010000112        13651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPHJE010000112 DAPHJE010000000
VERSION     DAPHJE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.113"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..918
                     /locus_tag="RH222_003515"
     CDS             <1..918
                     /locus_tag="RH222_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV3798477.1"
                     /translation="IEAYRLLHPRRQAWALRDYKDYIYHGPNFYLPHKLERAVTTFHD
                     ISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSRSEIIRLFNYPEERIVTTK
                     LACSSDYIPRSPAECLPVLQKYQLAWQAYALYIGTMEPRKNIRGLLHAYQLLPMEIRM
                     RYPLILSGYRGWEDDVLWQLVERGTREGWIRYLGYVPDEDLPYLYAAARVFVYPSFYE
                     GFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDIDAISAQILQSLQDDSWRD
                     IATARGLAQAKQFSWENCATQTINAYKLL"
     gene            928..2043
                     /locus_tag="RH222_003516"
     CDS             928..2043
                     /locus_tag="RH222_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV3798478.1"
                     /translation="MRVLHIYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KDTGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKLIKDDYDVINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLRGVDCIVASSPNYVASSQTLKKY
                     LDKTVVIPFGLEQQDVQHDPQRVAYWRETVGDKFFLFVGTFRYYKGLHILMDAAERSR
                     LPVVVVGGGPLESEVRREAQQRGLSNVMFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(2082..2690)
                     /locus_tag="RH222_003517"
     CDS             complement(2082..2690)
                     /locus_tag="RH222_003517"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV3798479.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNIVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(2684..3460)
                     /gene="hisF"
                     /locus_tag="RH222_003518"
     CDS             complement(2684..3460)
                     /gene="hisF"
                     /locus_tag="RH222_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV3798480.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDTDVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(3442..4179)
                     /gene="hisA"
                     /locus_tag="RH222_003519"
     CDS             complement(3442..4179)
                     /gene="hisA"
                     /locus_tag="RH222_003519"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV3798481.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(4179..4769)
                     /gene="hisH"
                     /locus_tag="RH222_003520"
     CDS             complement(4179..4769)
                     /gene="hisH"
                     /locus_tag="RH222_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV3798482.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(4769..5836)
                     /gene="hisB"
                     /locus_tag="RH222_003521"
     CDS             complement(4769..5836)
                     /gene="hisB"
                     /locus_tag="RH222_003521"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV3798483.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTALALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(5836..6906)
                     /gene="hisC"
                     /locus_tag="RH222_003522"
     CDS             complement(5836..6906)
                     /gene="hisC"
                     /locus_tag="RH222_003522"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV3798484.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(6903..8207)
                     /gene="hisD"
                     /locus_tag="RH222_003523"
     CDS             complement(6903..8207)
                     /gene="hisD"
                     /locus_tag="RH222_003523"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV3798485.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTSPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            complement(8213..9112)
                     /gene="hisG"
                     /locus_tag="RH222_003524"
     CDS             complement(8213..9112)
                     /gene="hisG"
                     /locus_tag="RH222_003524"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV3798486.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGKMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     misc_feature    complement(9159..9282)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(9258..9308)
                     /gene="hisL"
                     /locus_tag="RH222_003525"
     CDS             complement(9258..9308)
                     /gene="hisL"
                     /locus_tag="RH222_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV3798487.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            9591..9842
                     /gene="yefM"
                     /locus_tag="RH222_003526"
     CDS             9591..9842
                     /gene="yefM"
                     /locus_tag="RH222_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV3798488.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            9839..10093
                     /gene="yoeB"
                     /locus_tag="RH222_003527"
     CDS             9839..10093
                     /gene="yoeB"
                     /locus_tag="RH222_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV3798489.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            10176..11000
                     /locus_tag="RH222_003528"
     CDS             10176..11000
                     /locus_tag="RH222_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3798490.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            11046..11975
                     /locus_tag="RH222_003529"
     CDS             11046..11975
                     /locus_tag="RH222_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3798491.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            12190..12252
                     /gene="yoeI"
                     /locus_tag="RH222_003530"
     CDS             12190..12252
                     /gene="yoeI"
                     /locus_tag="RH222_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV3798492.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            12242..13600
                     /gene="plaP"
                     /locus_tag="RH222_003531"
     CDS             12242..13600
                     /gene="plaP"
                     /locus_tag="RH222_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV3798493.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVIYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT         3071 a         3569 c         3620 g         3391 t
ORIGIN      
        1 attgaggctt atcgcttgct gcatccgcgg cgccaggcgt gggcattgcg cgactataag
       61 gattatatct accatggccc caatttttat ctgccgcata aactggaacg cgccgtgacc
      121 acgtttcatg acatatccat ttttacctgc ccggaatatc atccaaaaga tcgggttcgc
      181 tatatggaga agtccctgca tgagagtctg gattcggcaa agctgatcct gaccgtttct
      241 gatttctcgc gcagtgaaat tatccgcttg ttcaactatc cggaggagcg gatcgtaacc
      301 accaagctag cctgcagcag tgactatatc ccacgcagcc cggcagagtg tctgccggta
      361 ctgcagaaat atcagctggc gtggcaggcc tacgcgctat atatcggcac tatggagcca
      421 cgtaaaaata tccgaggcct gctgcatgcc tatcagctgc taccgatgga gatccgcatg
      481 cgctatccgc taatccttag cggctatcgc ggctgggaag acgatgtgct gtggcagtta
      541 gtcgagcgcg gtactcggga aggctggatc cgttacctcg gatatgttcc ggatgaagac
      601 ctgccgtatc tgtacgcagc ggccagagtc tttgtttatc cctccttcta cgagggattc
      661 ggtttaccta ttcttgaagc gatgtcttgc ggtgtgccgg tagtatgctc caatgtcacc
      721 tctttgcctg aggttgttgg cgatgccggc ctcgttgccg atcctaatga tatagacgcg
      781 attagcgcgc aaattttgca gagcctgcag gatgatagct ggcgggacat cgccaccgcg
      841 cgcggtcttg ctcaggcgaa acagttttcg tgggagaact gtgcgaccca gaccattaac
      901 gcctataaat tactctaagg gtgtcagttg agagttctac acatctataa gacttactat
      961 cccgatacct acggcggtat tgagcaggtc atttatcagc taagtcaggg ctgcgcccgc
     1021 cggggaatcg cagccgatgt tttcactttt agcccggaca aagatacagg tcctgtcgct
     1081 tacgaagatc atcgggtcat ttataataaa cagctttttg aaattgcctc cacgccgttt
     1141 tcgctgaaag cgttaaagcg ttttaagctg attaaagatg actacgatgt catcaactac
     1201 cattttccgt ttccctttat ggatatgctg catctttcgg cgcggcctga cgccaggact
     1261 gtggtgacct atcactctga tatagtgaaa caaaaacggt taatgaagct gtaccagccg
     1321 ctgcaggagc gatttctcag gggcgtagat tgcatcgttg cctcgtcgcc caattacgtg
     1381 gcttccagcc agaccctgaa aaaatatctg gataaaacgg tggtgatccc gtttggtctg
     1441 gagcagcagg acgtacagca cgatccgcag agggtcgcgt actggcggga aactgtcggc
     1501 gataagttct ttctcttcgt cggcactttc cgctactaca aagggctgca tattctgatg
     1561 gatgccgctg agcgtagccg actgccagtg gtggttgtag ggggcgggcc gctggaatcg
     1621 gaagtgcggc gtgaagcgca gcagcgcggg ctgagcaatg tgatgtttac cggcatgctc
     1681 aacgacgaag ataagtacat tctcttccag ctctgccggg gcgtggtatt cccctcgcat
     1741 ctgcgctctg aggcgtttgg cattacgtta ttggaaggcg cacgctttgc aaggccgctg
     1801 atctcttgcg agatcggtac aggtacctct ttcattaacc aggacaaagt gagtggttgc
     1861 gtgattccgc cgaatgatag ccaggcgctg gtggaggcga tgaatgagct ctggaataac
     1921 gaggaaacct ccaaccgcta tggcgaaaac tcgcgtcgtc gttttgaaga gatgtttact
     1981 gccgaccata tgattgacgc ctatgtcaat ctctacacta cattgctgga aagcaaatcc
     2041 tgagagttgc tacccccgcc gttgctgcgg gggccaacgc gttaatgccg attcttcaga
     2101 ttatcaatca cttctccgag atccagcccc tgatcctgca acaacaccag caggtgatac
     2161 atcaaatcag acgcctcgtt ggtcagctca aagcgatcat gtaccgttgc tgccagtgcg
     2221 gtttccacgc cttcttcgcc cactttctgc gcaatgcgtt tggtgccgct ggcatacagt
     2281 ttggcggtgt aggaggtttc cgggtcggca gatttacgtt cggcgagcag ttgttccagt
     2341 tgatacagga acagccactg gtgagcagtg tcgccgaagc agctgctggt gcctttgtgg
     2401 caggttggcc cgatgggatt cgccagcacc agtaaagtgt cgttgtcgca gtccggcgca
     2461 atatttacta tgttgaggaa attacccgac gtttcgcctt tggtccacag tcgctgttta
     2521 gtgcgcgaga agaaggtgac tttgccgctt tcgatggttt tgtccagagc ttccgggttc
     2581 atatagccca gcattaacac ttcgccagat accgcgtgtt gtacaatcac cggcatcagt
     2641 ccgtcggttt tttcccagtc cagttcgcga cgttgttgtt ctgttaacat atcctgatct
     2701 ccacgccctg tgttgccagg tacgctttta attcaccaat attgattatt tgtttgtgga
     2761 atacggaagc tgccagcgca ccgtcaacgt cggtatcgcg gaaggcttcg agaaagtgtt
     2821 ccatggtgcc tgcgccaccg gaggcaatca gcgggacgtg gcaaacttca cgcacttttt
     2881 tcagctgttc gaggtcgtaa ccgttacgca cgccgtcctg attcatcatg ttgaggacga
     2941 tttctccggc accgcgtttt tgcacttcct gcacccaatc gagtgtttcc cactgagtga
     3001 cgcgggtgcg gctttcatcg ccggtatatt gattcacatg atatttaccg gtttcggcgt
     3061 cgtaccaggt atcaatgccg accacaatac actgcacgcc aaagcgatcc gccaggcgag
     3121 taattaacgt cgggtccgcc agcgcagggg agttaatgga gattttatcc gcgccaaagg
     3181 aaagaatttt cgcggcatct tccagagact taatcccacc cgccacacaa aacggaatgt
     3241 cgatcacttc cgccacgcga gatacccagc ttttatctac cacacggcca tcgctggaag
     3301 cggtgatatc gtagaacacc agttcatctg caccttcttc ggcgtagcgt tttgccagtg
     3361 gcacgatatc gccaatgatt tcatggttgc gaaactgtac gcctttcacc acctgaccat
     3421 cacgaacgtc gagacaaggg attatgcgtt ttgccagcat gcgatggcct ccttcacggt
     3481 gaatttacct tccagtaatg cacgaccaac tattacgccg cgtacaccag taccacgcag
     3541 ggccgccaca tcgttaatgt cgccaatacc gccggaggac tgaaatgcca cctgcggata
     3601 tctggcgcac acttcttcat ataaagagac gttagatccc gccaacgtgc cgtcgcgcga
     3661 gatatcggta catagcacat gtttcaggcc gacgggaaga taggtttcca ccagttgttc
     3721 cagtgaaacg cccgagttct cttgccagcc gctcaccgcc acctgcttgt taccttgctc
     3781 gtcaatacgg acatccagcg ccagcactaa ggcatcggca ccgaagcgtt caaaccagcc
     3841 tttcaccatt tctggtgatt tcaccgcggt ggagccgacc actacgcgcg caacgcccgc
     3901 ttccagtaac gccgccacgt cttcttcgct acgcacgccg ccgccaacct gcaccgggac
     3961 gttaacgccc gcgaccaggg ttttaatcag cgggatttga cgtttagccg gatcttttgc
     4021 cccggtcaga tccaccagat gcaacacttc ggcaccttgc gcagcgtaat cctgtaagcg
     4081 tggcagcggg tcgttaccgt aatcgcgttg tttgccgtaa tcgccctgat ggagacgcac
     4141 cacggtgccg tcgattaaat ctaatgccgg aataatcatc acatctccag gaagtttttc
     4201 agcaacttag cgccagcggc accagaacgc tccgggtgga actgcacgcc gtagaagtta
     4261 tctttttgta ccgccgcggt gaacggttcg ccgtaattac actgggcgat ggtccacgga
     4321 ttgaccggca ttgcgtagct gtgaacaaag taaaagtacg cgccgtcttc aatcccctga
     4381 aacaggcggt tgcctgcctg cggataaacg cggttccagc ccatatgtgg cagtggcaga
     4441 ccaaagtcgg tcattttcgg cacgtcttcg tcgatgatgc ccagcaagtc gacgccgttg
     4501 ctctcttcgc tgcgccgccc cagaagttgc atccctaagc agatgcccag caccggttgg
     4561 gtacaggctt tgatgagatc aaacagctcg cgctcacgca cctggtccat cgccgcttgc
     4621 gcggtgccaa cgccgggtaa aaacagttta tcggccagca acacgacgtc cgggtcacgg
     4681 ctgactttgg gttcataacc gtggcgcgca atggcagact tcaccgagtt caggttggcg
     4741 cagccagtat caaggatcac cacgttcatt acagcactcc tttcgacgag ggcagggtgt
     4801 cgccttccac gcggatggcc tggcgcaggg tgcggccaaa cgctttgaac aggctctcta
     4861 cacggtggtg atcgttttta cctttggttt tcaggtgtag cgtcacgccc atggtgtatg
     4921 aaagcgaacg gaagaagtgc tcgatcattt cagtgctgag atcgcccaca cgctggtagg
     4981 taaactcggc tttatattcc aggtgcgggc gaccagagat atccagcgcg cagcgggcaa
     5041 ggcattcgtc catcggcagc acaaaaccaa agcggcaaat accgcgtttg tctccgaggg
     5101 caatttttag cgcttcgccc agcgccagag cggtatcttc gacggtgtgg tgatcgtcga
     5161 tatagaggtc gcctttgacg ttgatttcca tgcggaaacc gccgtgagtg gcgatctgat
     5221 ccagcatatg atcaaagaag ccaacgccgg tgttaatctt gctgccacct tcgcgatcca
     5281 gccatacctg aacgtcaatc tgcgtctctt tggtgttgcg cactacatgg gcgtaacggt
     5341 ctcgtttagt gagttgctcg ccaatcatcg gccagttcag ggtttcgcgg tcgtagcgta
     5401 aaccattaat acccatgttt tccgccagtt gaatgtcggt cgcgcgatcg ccaatcacat
     5461 aactgttagc gcgatccatc gcttgctcag ccagataacg ctccaccagt tttactttcg
     5521 gtttacggca gtcgcactca tctgcgggca ggtgcgggca aatcagcact tcatcaaact
     5581 gtacgccttg cgaggtgaag atctgcatca tcaggttgtg cgggccatcg aaatccgcct
     5641 gcgggaaact ttgtgttccc agaccatcct gattagtgat catcaccagc ttgtagcccg
     5701 ctttttgcag cttcagcagc tctgggatca cgcccggttc aaaggcgagt ttgtcaaaac
     5761 ggtccacctg aaaatcactc ggcggttcgc taatcagggt tccatcgcga tcgataaaaa
     5821 gatacttctg actcattaaa cttgctccgc acgtaaggcg tcaatgacgc gctggctttc
     5881 ttcacgggtt ccgacggtaa ttcgcaggca gccgcttaaa gagggttgtt tattctgatc
     5941 acgtaagata atgccctgat cccacaaaga tttaaacact gcgctggagg ctttaaagcg
     6001 cgccagaatg tagttggttt cagagtcgaa aacctgctcc acgcagggga tctctttcag
     6061 tgcggcaatc agatattcgc gttctgcaat aatttgcgcc acccgctcac gcatggcgac
     6121 gatcccctgc gggcttaacg cctgggccgc aatgtcggca accggcgtcg agagcgggta
     6181 gggggcgatc actttcatta acaggttgat gacctcttcg tttgccagcg taaatccgca
     6241 acgaagcccc gccagagcaa aagctttcga cagtgtgcgc aaaatagcca ggtgcggata
     6301 ttccgccagc cagccagcca gcgatgcctg tggacaaaac tctatatagg cttcatcggc
     6361 aaccacaatc gccttaccgc gcgttaactc cagcagggtg cgaaaatcct gcgggttgat
     6421 cagttgcccg gtcgggttgt tggggctgca aacatagacc acttttacgc cgtccagctt
     6481 gtcggaaatg ccctgtaagt ccagttgcca gttgtccagc gtcggcactg tgcggcactc
     6541 gacgccaatc gtttcggcgc tgacgctgta catgccgtac gttggcgggc agtagaggat
     6601 ggcgtcttta cccggttcgc aaaaagcgcg aatcagcagt tcaatacctt cgtccgcgcc
     6661 acggctgacc agcacctgct ccggttttac gcctgcatat tgcgcgtaat tttcaatcac
     6721 cgctttcggc tggcattccg ggtagcggtt gagcgtttgc tgagtaagct gaaactccac
     6781 ggcagtgggg tattcgttgg cgttcagcca gacatcgccg ttaccgccca gacgacgcgc
     6841 tgactgatac ggcgtcaggt tgcggacgtt ttcacgcgct aaatcggtaa tagtcacggt
     6901 gctcatgctt gctccttaag ggcgttaaca cgcaaagtaa cggcattttt atgggcggtc
     6961 aggcgctcgg cggaggccag tgtttcaatg gtcgaagcca gcgcggaaaa gccttctttc
     7021 gagagttcct gcacggtcat gcgcttctgg aaatctgcca gcccgaggct ggaacaggtg
     7081 gcggtgtaac cgtaagtcgg tagaacatgg ttagtgccgg aggcatagtc gcctgccgat
     7141 tccggtgacc agtcaccaag aaataccgaa ccggcgctgg tgatgccatc gaccagttcg
     7201 cgggcgttgc gggtctgaat gatcaggtgc tccgggccgt actggttgga aatctctacg
     7261 cactgcgcta aatctttagt cacgatcagg cggctggcgc tcagtgcctg acgtgcggtt
     7321 tcggcacgcg gcagttctgc cagctggcgt tcgacagctt cggcaacgcg acgcgccata
     7381 tcagcatcgg gcgtcagtaa aatcacctga gagtccgggc cgtgttcagc ctgagaaagc
     7441 aaatcagaag ccacgaaatc tggcgtagcg ccgctgtcgg caatcaccag cacttccgac
     7501 gggcctgcgg gcatatcgat cgccgcaccg tccagacgct ggcttacctg gcgttttgcc
     7561 tcggtgacaa aggcgttgcc tggcccgaag attttgtcca cttttggcac agattcagta
     7621 ccaaacgcca gtgcggcaat ggcctgtgcg ccgccgacgt taaacacgtc ctgtacaccg
     7681 cacagctgcg ccgcataaag gatctcatcg gcaatcggcg gcggtgagca cagcaccact
     7741 tttttacagc ccgcaatacg cgccggagtt gccagcatta ataccgttga gaagagcggg
     7801 gcggagccgc caggaatata caaacctacc gaagccaccg ggctcgtgac ttgctgacaa
     7861 cgtacgcctg gctgagtttc tacatctacc ggcggcagtt tttgcgcagt gtggaaggtt
     7921 tcaatattct ttactgccac cgccatcgcc tgttttagct cgtcgctcag gcgttcgctg
     7981 gcggcggcaa tttcctctgc agacaccttc agcgcggtaa ccgtggtttt atcaaacttc
     8041 gcgctgtatt cccgcagggc ctcatcgccg cgtgctttca cgttatcgag aatatcgtta
     8101 acagtgcggg taatgctttc agaagcggag atcgccgggc gcattaatag ctggcgttgt
     8161 tgctccgcag tacagctatt ccagtcaatg attgtgttaa agctcatggc gatcactcca
     8221 tcatcttctc aatcggcagg accaggattg aactggcacc cagcgctttc agtttttcca
     8281 tcgtttccca gaacagggtt tcgctactga ccatgtgcat cgctacgcgt tgttggtcac
     8341 ccgccagcgg cagaatggtt gggcgttcgg cacctggcag cagggcgatg acttcatcca
     8401 gacgttcggt cggtgcgtgc atcatgatgt attttgattc gcgcgcctgg atcacaccct
     8461 gaatacgggt cagcagtttg tcgatcagtt gctgtttgga ttcttccatt ttgccatcgc
     8521 gctggatcag gcaggctttc gagcggtaga taacttcgac ttcgcgcagg ccgttagctt
     8581 ccagcgtggc accggtggaa accagatcgc aaatcgcatc cgccagtccg gcacgcgggg
     8641 cgacttcaac agaaccgttc agtaagcagg atttaaaaga gatgcctttc tggtcgagat
     8701 aacgcttgag caggtgagga taagaggtgg cgatacgttt accgtttaag gagagcgggc
     8761 cgtcccaggc ttcatcaact ggcgttgcca gcgaaagacg gcagccgccg aaatccagac
     8821 gacgcagggt aaagtagcgt ggatcttcac cctgggcgcg gcggttaagc agctcttctt
     8881 ccagcacgtt ttcgccgata atcccaaggt caaccacgcc atccattacc agaccgggaa
     8941 tgtcgtcgtc acgcacgcgc agaatatcaa tcggcatgtt ttctgccatc gcgatcaggc
     9001 gctgggtgtg aagattgatt ttaatgccac agcgcgccag caattcgcgt gagtcatcac
     9061 ttaaacggcc ggatttctgc atagctatgc gtaaacgagt gttgtctgtc attttcttat
     9121 tcctctttaa acctgtctga accgttatca accgcgcaat aaaaaagccc ccggaaggtg
     9181 atcttccggg ggctttctca tgcgttcatg caccactgga agatctgaat gtcttccagc
     9241 acacatcgcc tgaaagacta gtcaggatga tggtgatgat ggtggtgttt aaattgaacg
     9301 cgtgtcataa aagtctctgt gaatgtttat tcaactgatg tcttttaacc taaaccactt
     9361 tcacgttaga aagcaagggc ttttttatat attttatggc gatggatttg tttaatgaat
     9421 gagcggggaa aggagggggc taagaggact gtcctctaac ttgacagcac caaaaagagc
     9481 aaagtctgat ttttcgtctc gctggcaatg tcttgatcta caaactaatt aataaatagt
     9541 gaattaacac ccatcattgt acaatgaact gtacaaaaga ggagatttac atgcgtacaa
     9601 ttagctacag cgaagcgcgt cagaatttgt cggcaacaat gatgaaagcc gttgaagatc
     9661 atgccccgat cctcattact cgtcagaatg gagaggcttg tgttctgatg tcactcgaag
     9721 aatacaactc gctggaagag acggcttatc tactgcgttc ccccgctaac gcccggagat
     9781 tgatggactc aatcgatagc ctgaaatcag gcaaaggaac ggaaaaggac attattgagt
     9841 gaaactaatc tggtctgagg aatcatggga tgattatctg tactggcagg aaacagataa
     9901 gcgaattgtt aaaaagatca atgaacttat caaagatacc cgcagaacac catttgaagg
     9961 taaggggaaa ccagaacccc tgaaacataa tttgtcaggt ttctggtccc gacgcattac
    10021 agaggagcac cgtctggtat acgcggttac tgatgattca ctgctcattg cagcatgtcg
    10081 ttatcattat tgaacagatt cattcataga atgaattgtc agttttgata cgctagcgta
    10141 gccttataga tctaaggtgt atcaggagat aacggatgaa aaaggtcgca attgtcgggt
    10201 tagggtggtt aggcatgccg ctggcgatgt cactttcagc gcgaggctgg caagtcaccg
    10261 ggagtaaaac cacacaagat ggtgtcgaag cggcccgaat gagtggcatt gatagctatc
    10321 tgcttcgcat ggagcctgag ttagtttgcg attctgacga tctggatgcc ctgatggatg
    10381 ccgatgcgct ggtcattacg cttccggcac gtcgtagcgg ccccggcgat gagttctatt
    10441 tacaagcggt acaagagtta gtggatagcg cgctggccca tcgtattccc cgcattattt
    10501 ttaccagctc gacatctgtc tatggcgacg cgcaaggcac ggtgaaagaa accaccccgc
    10561 gtaatccggt gaccaacagt ggacgagtat tagaagaact cgaagactgg ctgcacaatt
    10621 tacccggtac ttcggtcgat attctgcgtc ttgcgggcct ggtcggaccg ggacgtcatc
    10681 ccggacgctt ctttgccgga aaaaccgccc ctgatggtga acatggtgtt aatttagtcc
    10741 atttagaaga tgttattggc gctatcactc tgttgttaca ggcacctaaa ggcggacaca
    10801 tctataatat atgtgcgcca gctcaccctg cgcgtaatgt tttctatccg cagatggccc
    10861 gtttattggg gctggaacct ccgcagttca gaaatagtct ggacagcggc aaaggtaaga
    10921 ttattgatgg cagtcggatt tgtaatgaac tgggatttga ataccagtat cccgatccgc
    10981 tggtaatgcc gctggagtaa acgatcacgc cagcaggacg ccgcatatac tgcaaggggg
    11041 cgtgtatgaa accactgctg gatgtgttga tgatcctcga tgccttagaa aaagaaggca
    11101 gttttgcggc ggcgtcggcc aaactctaca agacgccgtc cgctctgagt tacaccgttc
    11161 acaagctgga aagcgacctt aatattcaat tgcttgatcg cagcggacac cgcgctaaat
    11221 tcacccgcac cggaaaaatg ttattagaga aagggcggga agttctgcat accgtgcgag
    11281 aactggaaaa gcaggcgatc aaactgcatg aaggctggga aaacgagctg gtgattggcg
    11341 tggacgacac cttccctttt tctctgcttg cgccactcat tgaggctttt tatcaacatc
    11401 atagcgtgac gcgcctgaag tttatcaatg gtgtactcgg tggttcatgg gatgccctga
    11461 ctcaggggcg agcggatatt attgttggcg cgatgcatga gccaccatct tccagtgagt
    11521 ttggtttctc tcggttgggc gatctggaac aagtctttgc ggtcgcgcct catcatccgt
    11581 tagccctgga agaagaaccg ttaaaccgtc gtatcattaa acgatatcgg gcgattgtgg
    11641 tgggggacac tgcacaggcc ggcgcttcta cggcctcaca acttctcgac gaacaagaag
    11701 ctattaccgt tttcgatttt aaaaccaagc tggagctgca aattagcggc ctcggctgcg
    11761 gctatttacc ccgttatctg gcgcaacgtt ttctcgatag tggtgcgtta atcgagaaga
    11821 aagtggtcgc ccaaaccctc tttgaacccg tctggattgg ctggaatgaa cagaccgcgg
    11881 ggcttgccag tggttggtgg cgggacgaaa ttttagcaaa tagtgcgatc gccggtgttt
    11941 atgcaaaatc tgatgacgga aaatcagcca tttaaagaaa aattattatg acaagcctct
    12001 cattctcttg tcatttcccc cccatttagg cacaatgcgc cgctgtcaaa aaatgactaa
    12061 aaaccgacgt ttcattagcg tcggttattt tttgcttcaa accaatcatt cataccaaga
    12121 ggccgggctt cgtaccggat agatatttac taaaaatcga cagttgttgt cgctgaggaa
    12181 tccaaaaaaa tggggcaatt ttttgcttac gcgacggtta tcaccgtaaa ggagaatgac
    12241 catgtcgcat aacgttactc caaacacctc tcgcgtggaa ttgcgtaaaa cgcttacgtt
    12301 agttccggtt gtaatgatgg gtcttgccta tatgcagccg atgacgctgt ttgatacatt
    12361 tggtatcgtt tcaggcctca cggatggtca tgtgccgaca gcctatgcgt tcgcattgat
    12421 tgcgatcctg tttacggctc tgagctacgg gaagctggtt cgccgctatc cttctgctgg
    12481 ctctgcatac acttacgccc agaaatccat tagcccgact gtcggcttta tggtgggttg
    12541 gtcttctctg ctcgactatc tgttcgcgcc gatgatcaac attctgctgg cgaaaatcta
    12601 ttttgaagct ctggtgcctt ccatcccatc gtggatgttt gtggtggcgc tggtggcctt
    12661 tatgaccgcc tttaacctgc gtagtctgaa atccgtagcg aacttcaaca ctgtaatcgt
    12721 cgtgttgcag gtagtgctga tcgcggtgat tctgggcatg gttatttatg gcgtatttga
    12781 aggtgaaggc gcgggtacgc tggcgagcac tcgtccgttc tggtctggcg atgcgcatgt
    12841 tatcccgatg attaccgggg cgacaatcct gtgcttctcc tttaccggct ttgacggcat
    12901 cagcaacctg tcggaagaaa ccaaagatgc agagcgcgtg atcccgcgtg cgattttcct
    12961 gaccgcgctg attggcggca tgatcttcat ctttgcaact tacttcctgc agctgtactt
    13021 cccggatatc tctcgcttta aagatccgga tgcgtcacag cctgaaatca tgctgtacgt
    13081 tgcgggtaaa gcgttccagg ttggcgcgct gatcttctcc accattaccg tactggcgtc
    13141 cggtatggcg gcgcatgcag gcgtagcgcg tctgatgtac gtaatggggc gtgacggtgt
    13201 gttcccgaaa agcttcttcg gttatgtaca cccgaaatgg cgtactccgg caatgaacat
    13261 catcctggtt ggcgcgattg ccttgctggc aatcaacttc gacctggtaa tggctacggc
    13321 gctgattaac tttggtgcgc tggtggcgtt caccttcgtt aacctgtcgg ttatttcgca
    13381 gttctggatc cgtgagaagc gtaacaagac gctgaaagat cacttccagt atctgttcct
    13441 gccgatgtgt ggtgcgctga ccgttggtgc gctgtgggtt aacctggaag aaagctcaat
    13501 ggttctgggt ctgatctggg ctgctatcgg tctgatttac ctggcttgcg taaccaagag
    13561 tttccgtaat ccggttccgc agtacgaaga cgtagcataa tcatcttttc taataataaa
    13621 ccggagtcga atgaacattt ggctccggtt t
//
LOCUS       DAPHJE010000113        13436 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPHJE010000113 DAPHJE010000000
VERSION     DAPHJE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.114"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            124..954
                     /locus_tag="RH222_003532"
     CDS             124..954
                     /locus_tag="RH222_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HDV3798494.1"
                     /translation="MSMPLSNALQSQIITDAHFLHHPIVDSEFTRKLKYARMDSENIY
                     LPPLTRGNNHNYDGKSVVEIRKLDISKEPWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHATTELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1095..1868)
                     /gene="uxuR"
                     /locus_tag="RH222_003533"
     CDS             complement(1095..1868)
                     /gene="uxuR"
                     /locus_tag="RH222_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV3798495.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2083..3543)
                     /locus_tag="RH222_003534"
     CDS             complement(2083..3543)
                     /locus_tag="RH222_003534"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV3798496.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETIQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTVTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3624..4808)
                     /gene="uxuA"
                     /locus_tag="RH222_003535"
     CDS             complement(3624..4808)
                     /gene="uxuA"
                     /locus_tag="RH222_003535"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV3798497.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIIEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQATE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5148..6491
                     /gene="gntP"
                     /locus_tag="RH222_003536"
     CDS             5148..6491
                     /gene="gntP"
                     /locus_tag="RH222_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV3798498.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6734..7636)
                     /gene="fimH"
                     /locus_tag="RH222_003537"
     CDS             complement(6734..7636)
                     /gene="fimH"
                     /locus_tag="RH222_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV3798499.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7656..8159)
                     /gene="fimG"
                     /locus_tag="RH222_003538"
     CDS             complement(7656..8159)
                     /gene="fimG"
                     /locus_tag="RH222_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV3798500.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8172..8702)
                     /gene="fimF"
                     /locus_tag="RH222_003539"
     CDS             complement(8172..8702)
                     /gene="fimF"
                     /locus_tag="RH222_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV3798501.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8712..11348)
                     /gene="fimD"
                     /locus_tag="RH222_003540"
     CDS             complement(8712..11348)
                     /gene="fimD"
                     /locus_tag="RH222_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV3798502.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSFNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LITLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11415..12140)
                     /gene="fimC"
                     /locus_tag="RH222_003541"
     CDS             complement(11415..12140)
                     /gene="fimC"
                     /locus_tag="RH222_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV3798503.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12177..12716)
                     /locus_tag="RH222_003542"
     CDS             complement(12177..12716)
                     /locus_tag="RH222_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3798504.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHVVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12781..13329)
                     /gene="fimA"
                     /locus_tag="RH222_003543"
     CDS             complement(12781..13329)
                     /gene="fimA"
                     /locus_tag="RH222_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3798505.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3270 a         3490 c         3239 g         3437 t
ORIGIN      
        1 ccgtgtagag ttgatatcac tacaacagaa acatgcatgt catttgttta cttctctttt
       61 tactacagaa aaatttatct atttttctaa acatcagtga aatactcata aaaggtaact
      121 attatgtcta tgccattaag caatgccctt cagagtcaaa ttattacaga tgctcacttc
      181 cttcatcacc caatagtcga cagtgagttt acacgtaaat taaaatatgc acgcatggat
      241 tcggaaaaca tctatcttcc gcctcttact cgtggcaata accataatta cgatggaaaa
      301 agcgttgtcg aaattagaaa acttgatatt agtaaagagc cttggccatt taattatgtg
      361 acaggagcat gcagagaatc cgatggtatt acaacaacag gacgtatgct ctatcggaac
      421 ttgaaaatca cctcagcttt agacgaaata tatggtggca tatgtaaaaa ggcgcatgct
      481 accacagagt tagcagaagg tttacggcta aatttattta tgaagagccc tttcgatcct
      541 gttgaggact atacagttca tgaaataaca ttaggccctg gttgcaatgt tcctggttat
      601 gccggaacca cgataggata tatttcaacc cttccagctt ctcaggcaaa aagatggacc
      661 aatgagcagc caaggattga tatctatatt gatcagataa tgacggtaac aggggtagca
      721 aattcttccg gattcgccct tgccgcatta cttaatgcta atattgagtt gggtaatgac
      781 ccaataattg gtatagaagc ctatcccggt accgcggaga tccatgcaaa aatgggatat
      841 aaagttatcc cgggtgatga aaatgcacca ctcaaacgta tgactctgca accgtcatct
      901 cttccagaac tgtttgaatt aaaaaatggt gaatggaatt atattggtaa ataactacca
      961 ggaagaatga gtactaacat tcaggttata cagatagcta aacaataccc tgcaatttac
     1021 aaagtaatac tcattgcgac ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg
     1081 cttattataa taagtcaggc gtcgacttta tccagcggcc atgaatcaaa cagatagcca
     1141 tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgcttaac gttttccaga
     1201 tgctgccaca ttgccagctt cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc
     1261 tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catccagatg gctgtgcaac
     1321 tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaacag ctccaccagc
     1381 atgctgttat gcgttgcttc ggcaatagcg agatggaact gcatgtcacc gctttcgctg
     1441 ctgcccggcg cactggaagc cagttcacgc tcttccagtt gcaatgcctg acgcattttg
     1501 acgatatctt cgcgggtagc ctgcaaagcg gcaaactcgg cgatgttgct ctccaataac
     1561 tgccgcgcct gtaacagctc aaaaggaccg gcatcgttgc agacgttggc atccggactg
     1621 tctgtgttct ggctgcctga gttatcaaga acatagatac cggcaccccg gcgtacttcc
     1681 accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg cgtgacatca
     1741 agcatttctg caatttcacg ctccggcggc agccgttcgc caggattgta cggcgtcttt
     1801 atgatcagat cgcggatcat cgccccgact tcctggtaag gtctttgcgc agaggtggca
     1861 gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg
     1921 aacgccttat ccggcctaca acgggcaatt ttacggcccc cgttaatctc aaactatacc
     1981 attagcactg gaagttggca cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat
     2041 atcagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg cagccacaca
     2101 ctcgcgcgca ccgcgttcgc acagctgctg atatgccgcc gtcactgcgc caacaaagtc
     2161 ggcattctgc ggcagatcat cggcaaaaat accgctcagg cccagcagcg ctttcacgcg
     2221 gtctgcgccc tgatactgcg cgttgatctt ctggaactcc gccagcatcg ggtcgaccac
     2281 gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cgcatccagc cagccacgcc
     2341 cagcgccagg tgacgccagc tgccgccgtt ttgcaggtgc agacgcaccg ggtccagcag
     2401 acgctgcggt aacttctggc tgccgtccat cgcaatctgc caggtacggt gacgcagaga
     2461 cgggttgctg aaacgctcga tcagcagcgt cgcataggcg ttcaggtctg taccttccgg
     2521 catcgacagc gttggcgctt gttcctgcat catcagggca aaggccgctt tgcgataagc
     2581 cgggttagtc acggtgtcgg caatggtttc atagccgccg aggtaaccga ggtacgccag
     2641 aaaagagtgg ctgccgttca gcatacgcag cttcatcatt tcgaacggca caacgtctgc
     2701 aacgaactgt gcgcccactt tatcccaatc cgggcgacca ttaacgaagt tatcttcaat
     2761 cacccactga cggaacggtt cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc
     2821 agcaatttcc tgtatggttt ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca
     2881 cgggaaggtg acattttctt caatccatgc cgccagctgc gggtcacgcg cctgagccag
     2941 ccccagtacc gcgaccttcg ccacatgacc gttttcacgc acgttatcgc aggacatcac
     3001 cgtaaacgct ttcaaccctt tttcacgacg cagacgcaac gcttcgacga tgtaaccaat
     3061 cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg atcagcgggt tattgagatc
     3121 cagttgaccg cttgccgcat cagcgcagta gcctttttcc gtgaccgtta gagagacaat
     3181 cgccgtttgc ggacgcgcca tcgcgttgag aataccttcg cagccatcga tttccggatg
     3241 cagcgcttct ttcatcgaac cgataatttt cagctcggtg ctctctgcgc ctttttccgc
     3301 tacggtgtac agcagttgct gttttttcag gttttcgatc agcacgcggt cgttgcctgg
     3361 catcaggtta acttcgcaga tgccccagtc gctgtcggtg ctttccagca gatgatgggt
     3421 atacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc
     3481 cagacgagaa tgatcccatg acgggcgggc aaccggcaga ttgctgtcaa caatagtagt
     3541 catcgtaaac tccagaatcc gtcttcaaag agtgacggca aggaagaagg aaccggcacg
     3601 gcgcgcagcc atgccggtgg atattaacgg ctaaagaaag cgcgctggat cgccagttcg
     3661 acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc tgggttggtt
     3721 ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg gatcaggtct
     3781 tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac cacttcgtac
     3841 atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc
     3901 atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat catatcaacc
     3961 agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtaaaacc gtttgccatg
     4021 ctgtttacgg tatcaaccat ccactgcata tcttcaatgg tggaaacaat gcgcggcagg
     4081 ccgaggatcg ggcgcggcgg atcgtccggg tgaacagcca tacgcacgcc gacttcttca
     4141 gcaactggaa taatcgcttt caggaagacg gcaaagtttt cgcgcagctt cgctttgtcg
     4201 atatctttgt acagctccag gtgtttacgg aactggtcga gggtgtagcc ttcttccgcg
     4261 cccggaagac cagcaatgat gttacgggtc agacgcgctt tgtcttcatc gctcatagtg
     4321 gcgaagcgtt cagttgcctg agcaatttct tcttcggtgt aatccgcttc cgcgcctggg
     4381 cgtttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg cagagctttg
     4441 gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag
     4501 ttgtagcaca cggtgcgaat gccacactgc gccaggttgc gcagagtctg ctgatagtta
     4561 gcgatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat tggcacgctt
     4621 tctacgacag accacaccag gcctgcgtct tcaatgatcg ccttgcgttt gaggatctct
     4681 tcaacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac gccagttgcg
     4741 cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc
     4801 tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac gcggaaccct
     4861 ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg catcggatga
     4921 gatggcgtat aagttctacc ttttggtaag acatgtcaac ccaaattctg aaattggtta
     4981 accacatcac aagaatttca ctctcatggt tgttgctgca aattgcagca aagggtaatc
     5041 gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca atttacataa
     5101 catatcgccc aaaataacaa cggttcaacc aggtaaggta aaacgttatg catgtgctta
     5161 acattctctg ggtggtattc ggcattggtc tgatgctggt actgaatttg aagttcaaaa
     5221 tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt cgggatgctg gcgggcatgg
     5281 atttgatgtc gctgctgcac actatgaaag cgggcttcgg caacacgctg ggggaactgg
     5341 ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat ggtcgactcc ggcgcggctc
     5401 accagatagc gcatacgctg ctggcgcgtc tcggtctgcg ctatgtacag ctgtcggtga
     5461 ttatcatcgg cctgattttt ggtctggcga tgttctatga agtggccttt atcatgttag
     5521 cgccgctggt tattgttatt gccgccgaag ctaaaattcc gttcctgaaa ctggcgatcc
     5581 cggcagtagc agctgccact accgcacatt cactgttccc accgcagccg ggtccggtgg
     5641 cgctggtgaa tgcttatggc gcggatatgg ggatggttta tatctatggc gtactggtga
     5701 cgatcccaag tgtaatctgc gcaggtctga tcctgccgaa gttcctcggc aatcttgagc
     5761 gcccaacgcc atcattcctg aaagcagatc aaccggtaga tatgaataat ctgccctctt
     5821 tcggcgtttc gattctggtg ccgctgatcc cggcgatcat tatgatctct accaccatcg
     5881 ccaatatctg gctggtaaaa gatacccctg cctgggaagt ggttaacttt atcggttcct
     5941 cgccgattgc aatgtttatt gcgatggtgg ttgcattcgt actctttggc accgcacgtg
     6001 gtcatgacat gcagtgggtg atgaacgctt ttgaaagcgc ggtgaagagt attgcaatgg
     6061 tgattctgat catcggtgcg ggtggcgtgc tgaagcagac catcatcgac accggcattg
     6121 gcgacaccat cggcatgttg atgtcccacg gcaatatctc gccctacatc atggcatggc
     6181 tgatcactgt gctaattcgt ctggcgacgg gtcagggtgt cgtttcggcg atgaccgccg
     6241 ccgggattat cagtgctgca atcctagatc cagcaactgg tcagctggtt ggcgtgaatc
     6301 cggcgctgct ggtactggcg acggctgcgg gttccaacac cctcacccac attaacgatg
     6361 catctttctg gttgttcaaa ggttactttg acctgtcagt aaaagacacg ttgaaaacct
     6421 ggggtctgct ggagctggtc aactccgtgg ttgggctgat tattgtgctg attattagca
     6481 tggtagcgta aaaggacacc agagcctgcc aatggcaggc tcagactgat gacaaagtct
     6541 aaaaatgtgc ccggacagtc ccctcgcccc tccggggaga gggttagggt gaggggaaca
     6601 gaccagcaaa gtgcgctttg tttatgccag atgcgacgct gacgcgtctt atctggccta
     6661 caaagggcta acgtgcaggt ttttagcttc aggtaatatt gcgtaccagc attagcaatg
     6721 tcctgtgatt tctttattga taaacaaaag tcacgccaat aatcgattgc acattccctg
     6781 cagtcacctg ccctccggta cgtgcataat ttgccgttaa tcccagactc accgccgaag
     6841 tccctactgc tcctaacgat accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca
     6901 actgtacgcc gacgccctgt gcaggtgaaa acgacgcggt attggtgaaa atcgagttgc
     6961 ccgcatctgc ggttgtgccg gagaggtaat accccaggtt ttggcttttc gcacaataaa
     7021 cggtaagagg aattggcact gaaccagggt agtccggcag agtaacggtg acatcacgag
     7081 cagaaacatc gcagccgcca gtaggcacca ccacatcatt attggcgtaa atattccaca
     7141 caaactggaa atcatcgctg ttatagttgt tggtctgtcg caaaataagc acggcaatta
     7201 atgagccagc tttaatcgcc accccgcccg cactgctcac aggcgtcaaa taaagcgcca
     7261 ccggccacgg cttatccgtt ctcgaattat aaacaacgcg cggcgtttcg ctggtggtag
     7321 gaaatggata gctactgcca ctatatttta cggtcccgga aaaattagat aacacgccgc
     7381 cataagccga gcctcgttgc agtgtgacat agtctgtaat ggtttccgga taatcgttat
     7441 ggcaaaagat ttgcgtcgaa agatccacga ccaggttttg ccccacattc acgacgggcg
     7501 caaggtttac ataaacattg gcgctgccac cgccaatagg gatagcggta ccattggcgg
     7561 ttttacaggc gaatgaccag gcatttaccg accagcccat cagcagtaca gcaaacaggg
     7621 taataactcg tttcattaca atcatctctt cgggttcagc tgtaggtata ggtgatgcta
     7681 atcactgcct gaatggttcc ctgagtggct ccgccattta ctgtcaatgc tctgacctgt
     7741 aacgggaagt gcgctgattg tgaggaatca tccacctgaa ctgttttggt tgcgccagta
     7801 ttcaatgtgt tgccactgtc atcctgtagc tctaactgga tgttttgcgc ggtcccctgg
     7861 tttttataat atccggtact gtcggctgcc ccgctgaagc tggcagtgac cctcgacgtt
     7921 cccaccggac aattagtcaa ctcaagcgca acatcatgcc aggccgatgc cgccccggca
     7981 gacataagac tgaaagaata aagatcgccg agatcaaccg tggcattggt ggtggaaacc
     8041 gtacacggtt tggcgacgac cttaccgttc accgtgatgg tgacatcggc tgcctgtatc
     8101 gtcgcacttg cgagcgccaa tattgccgcc aatacatacc cacgtttgca ccatttcatg
     8161 agcatctcca gttactgata ttcaagagtg aaggtagccg tggcattgat atgccccgca
     8221 gtgacaggca cctgtgtcgc cattagccgg gcgtaaaaat tcagcgtatt tggtttaccc
     8281 ggcgtcaggg tcgtccacga aagcgcggac gatggagcat taaggggtat ttgattttgc
     8341 tgctcattca gaagctgtat tcccagtccc gaagccgctg acaccgtatt ttcaagtgca
     8401 agcaggttgg cattgtggct atctgcaacg ccagtaaacc caacctttac ggcagaaacg
     8461 gcattaccac agggtgacag caaaatacga aatggaacaa caggagtcgt cgcgccaatg
     8521 ttgttaaatt gcttcgccgc gttttccatc agatcaacag taaaattggt tgattcagcg
     8581 gccacactac agccgttatc cctgacatag ccgcggatag taatcgtgct atccgcagcc
     8641 aaagcgtgac tcaccgccag ccacaaaaaa gcgcacagaa gataaaaagg tttgtttctc
     8701 atcacgcccc cttaacgaca ttcagctgat agctgggtta ataactgctg ctgactctct
     8761 ggtggcagtt gataattggc gacacagtga gcattttcct cttctcccca tttcacctga
     8821 acttttcccg ctaaaggcat tccgctgagg taaacctgac cattatccgc aacaatgccg
     8881 ctactctggc tactctctga tgtcaccatc gccccaaacg gcagcggctt attattgtgg
     8941 gtcagcgtta tgagcagttt tatcccaacg cgcgctttaa actctgctcg cacgatcgcc
     9001 ccacgagtgg gaacaacgtt agcaaccgcg ttatctaaat cgacgttatc agccagggta
     9061 ttggtatcca gcgccactct attttcccga tattcagtgg cataaggcag cacggcataa
     9121 ccacgccagt cggtacgcac ccccgtctgg ttttcgactt ttgcatcttt tgcgccaggc
     9181 gctttaacaa gcaccaccgt atcgtttaac ggctgcccca gcgttacgcc attggcatga
     9241 gccagtaccc caccgctgac tccgtaatag agctgcttaa tatcatcgct atggctgtaa
     9301 ccgatattgg cattgccgta accaccgcga taattcagcg tggcgtagcc tgtacttccg
     9361 ctatttccat cgcctccccc ggcatagccg gtttgcacgc tatagctgag gttgttgtct
     9421 tccagcaacg taccgtatac accagccaga ttggtcatcc gaccgttgag atcgtgtgac
     9481 atgctgtagc tggcactggc atgtcgccac tgagatttac tgtcagaacg cagccagtgg
     9541 ctgaaaggaa tattgacgtt aagcgctaac atctgatccc gtcctttttg ccaggcgttt
     9601 ttcgtcaggc tatagctgag cgtccagttg atatcttcga acgcagtatt taatccagcc
     9661 tggaattgct catcgacatt actcgttccc caataagttt gatggctacc actcaaatac
     9721 agtgttgatg tgcgcccgag ttgctgagta acggtgagtt gtaatttccc gcgtttgtta
     9781 taagcgaggt tgtaatagtc ggtgaatttc ggcttaacct gaataactcc gtcctgtgtt
     9841 tcgatgttgt agccattcat tcgactgtat gttgtatcag cgaaattaaa atatccgctg
     9901 gtcgaataac ggtaacccac taactgaata ttcgtgcctg attcattgaa cgatttgtta
     9961 tagagaaaac gcaccgattg tccgtcatgc tgactgtcat cgggaagtgt ggaattagcc
    10021 tgcgtcatat ccacagacag agcgcccagt gcccccatgt ttttcccgat accgaaatta
    10081 aaagcacgat aacgatccgc cagttgcgtt ccaccatata ttgtccagcc agccggaagg
    10141 ccgtggagta atgtactctg gaaaaagcgg ggtttttcct gctgcgcatt tccactacgg
    10201 tattctcctg ccgtaatgga ataacgagta tgcccttcac gttgcaaaag cgggactgac
    10261 gaatagggta cggtaaaaat ctgcgtgctg ccgtcagcct ctttgatcgt tacctgcaag
    10321 tcaccactat tacctgcggc atagatatcg ttgatggtaa aaggccccgg tggcaccgta
    10381 ctattataaa tgtcataccc attttgttta atagtgacct gtgcagtacc acgagcaata
    10441 ccgtggatca ccggggcaaa tcctctttga ctatcgggta acatattgtc atctgaggcc
    10501 aattgtgcgc cgcgaaagtt aataccatcg aaaatatcgc cctgagtata accatcaccc
    10561 agcgtcagcc gggaacgtaa cggtattatg tctcgctcaa gccaggtatt gatatgctgc
    10621 catttatttt tgctacctga tgatctgtcg ctactgttat aactccaggt ggtattgtcg
    10681 cgtaaacgcc acgcaccaat atttaaccca ctctgtaggt ttaaatatgc ataatggctg
    10741 ttacccccaa tccgattctg tacactattt ccgctgaaat tataattgag caatccggca
    10801 ttaataccgg gatcccataa ctcaggagga atataaccac gcgcgcgatt actcataaat
    10861 gcctgaggga tcgtcaggtt cagtcgctgc tgaccaacat ccagatgcgc agtagcgtcc
    10921 tggaccattg tggttaatgg cacacaggca tcatccgcca gcagattcat accggcgaca
    10981 gaagccgtat tcagccccat actggcgagt tgcgcgcgtg tcaggcaggg aacaatccct
    11041 tgttcactgt cgcccgtatt aaatgtgaca tcacgcgttg ccatataacc attattcaaa
    11101 tagatatcga cgcgatacgt ccctggcggt aattcttgcc cattttcaaa acgcgataaa
    11161 tcggccacag cctggggatc atccgctaaa aagcgcggat taaaatagag gtcggcagat
    11221 gacaaaggtg cctgtgcggc aaaagcacag gcgacaacga gtcggacaaa aaaaccagcc
    11281 aaacgatgct tacgaatatg caagcattgt gtgtttcgct ggtaaagtct taaattcaga
    11341 tatgacatta ttcctccggc ttaaaatcac ccggcagtca attcttttta ttcaggcgaa
    11401 aaattccccc tgcgttattc cattacgccc gtcattttgg gggtaagtgc gccataatca
    11461 tttattgttc ggtaagtaat attgcttcct gcatcagaag gcaatttaac cgtgctttcg
    11521 cccattggag gcaccaatgc attttcaaga acccgggttc cggcattcaa ctctgttacc
    11581 gtcaggtaat agggtgtcgg gttaatcagc gtcagagaat tcgcgctacg acgaaatctt
    11641 aatttttctg cggcctgatc gggtggcaac gctaatttag ccgggcgata gtacagttta
    11701 atgcggctga taattgcgag ctgtagcgta ttctcagtca attttgattt atccattgac
    11761 ggaatcgctt taacgttcat ccagaataaa ctttcccggt cctgtggcaa ttggttattt
    11821 gttgcatcaa gaatacgtaa ggtattctct ttttttccct tcatcgcaaa cagaggaggc
    11881 gtcacgataa aacgaccatc ctttacacca tcggcatttt ccacccatga ttgaattaaa
    11941 taggtactat tttcatcatt atttgtcacg gcaagttgca cttgtttttg ccctgccgga
    12001 taaattacgc gagtcgcacc taaggccact cccgcttcag cgcgtccggc aaccatcatt
    12061 gccatgaaca tcaggatacc tgccagcaag cagaatgtta tttcctgcga tttccttaca
    12121 ttgacgtttt tattactcac tgtcctgttc ctgttatcac attatctgct gaacaattac
    12181 tgataggtta aagagaacca ggcctgggca ttggcgatgc cgccagtaac ccgacgcccg
    12241 gtagcacgat atttggcgat gaaatgtagc gaagtagagc ctgaataaag ccgtttccag
    12301 tttgctggag gacgattaat cggtacgagg tttccttcat catcaaacaa cgctacgcca
    12361 atattggtgg ctatccctgg cccctctccc acggaaagca catccggatt tttaccatcc
    12421 gcgacaccgt gaaacgccac acctacacgt tcactcacca ccgtgctaca ttcccgtaaa
    12481 tgaataacaa aaggcaccgg tgcgctatct tccccaacca catgaaaccg gttactgctg
    12541 atttgcccca tattgaccgt catttgttta tcaccggctt caatccggca agtttccgca
    12601 ataatgacgc cctgaaattg catatttccg ccgggcaacg tggtattcca tttatttccg
    12661 gccagagcaa acattggcag caacgacgct aatagaaata atctcttcct tttcatcgtt
    12721 atttttatcg cacaagggtg ggcatccctg cccgtaatga cgtccctgaa cctgggtagg
    12781 ttattgatac tgaaccttga aggtcgcatc cgcattagca gcacccgggg ttgcggcccc
    12841 ggttgcaaaa taacgcgcct ggaacggaat ggtattggtt ccgttattca gggttgtttc
    12901 tgaactaaat gtcgcaccat ccagcgtcag cgcagcaccc gttctgtcca ggatctgcac
    12961 accaacgttt gttgcgctac ccgcagctga actctgcaga gccagaacgt tggtatgacc
    13021 cgcatcaatc gccgtaccta aaaaggcaac agcggcttta gatgcaacat tggtatcgca
    13081 atcattcagc tgaatgttaa aaccgacagc agaactggtt gctccttcct gtgccagcga
    13141 tgcggtacga acctgtccta actgaacggt ttgatcaaca gagcctgcat caactgcgca
    13201 agcggcgtta acaacttccc ctttaaagtg aacggtccca ccattaaccg tcgtggcagc
    13261 ggccagagcc gctgtagaac tgagggacag agccgacaga acaacgattg ccagagtttt
    13321 aattttcatg ctgctttcct ttcaaaaaac tatttctaaa tcgacatggg cagtcgttct
    13381 gtacactttg ttttgtcaac gagtttgact gccaacactg cacagttttc ccccaa
//
LOCUS       DAPHJE010000114        13333 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPHJE010000114 DAPHJE010000000
VERSION     DAPHJE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.115"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..438
                     /locus_tag="RH222_003544"
     CDS             82..438
                     /locus_tag="RH222_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3798506.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(489..1385)
                     /gene="yhaJ"
                     /locus_tag="RH222_003545"
     CDS             complement(489..1385)
                     /gene="yhaJ"
                     /locus_tag="RH222_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV3798507.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1490..2191
                     /locus_tag="RH222_003546"
     CDS             1490..2191
                     /locus_tag="RH222_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV3798508.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2214..2378
                     /locus_tag="RH222_003547"
     CDS             2214..2378
                     /locus_tag="RH222_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798509.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2590..3900)
                     /locus_tag="RH222_003548"
     CDS             complement(2590..3900)
                     /locus_tag="RH222_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV3798510.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3928..5259)
                     /locus_tag="RH222_003549"
     CDS             complement(3928..5259)
                     /locus_tag="RH222_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3798511.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5534..6898)
                     /gene="tdcG"
                     /locus_tag="RH222_003550"
     CDS             complement(5534..6898)
                     /gene="tdcG"
                     /locus_tag="RH222_003550"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV3798512.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6970..7359)
                     /locus_tag="RH222_003551"
     CDS             complement(6970..7359)
                     /locus_tag="RH222_003551"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV3798513.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7373..9667)
                     /gene="tdcE"
                     /locus_tag="RH222_003552"
     CDS             complement(7373..9667)
                     /gene="tdcE"
                     /locus_tag="RH222_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV3798514.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGDDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9701..10909)
                     /gene="tdcD"
                     /locus_tag="RH222_003553"
     CDS             complement(9701..10909)
                     /gene="tdcD"
                     /locus_tag="RH222_003553"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV3798515.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10935..12266)
                     /gene="tdcC"
                     /locus_tag="RH222_003554"
     CDS             complement(10935..12266)
                     /gene="tdcC"
                     /locus_tag="RH222_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV3798516.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12288..13277)
                     /gene="tdcB"
                     /locus_tag="RH222_003555"
     CDS             complement(12288..13277)
                     /gene="tdcB"
                     /locus_tag="RH222_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV3798517.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3402 a         3626 c         3171 g         3134 t
ORIGIN      
        1 atccgaaaaa gaaagatacg aatagcattt acacaatacc cggaatagac tttcgtcact
       61 tttaattaaa gggatgtttt tatgcagtgg tatttagccg tattaaaaaa ttatgttggt
      121 ttctctggtc gcgcacgtcg taaagagtac tggatgttta ctctgattaa cgccatcgtc
      181 ggcgctatta tcaatgtcat tcaattgatt ttaggtttag agtttccatt cctttctctc
      241 atttacctgg cagcaacaat cattccggtc attgctctgt gtgttcgtcg tctgcacgat
      301 actgaccgtt caggtgcgtg ggcgcttctc tatctggtac cgatcattgg ctggttagtt
      361 ctgtttgtat ttgcctgttt agaaggcaat tctggcagca atcgttacgg aaacgatcct
      421 aagtttggtt caaattaatc ttagaattgg ggcgacattt cgcccctttt tattaataat
      481 aacaaatatt attttccgct aaaaagtttg ggaatttccc gcagacacca ggatttcgct
      541 tcccccatac tgtcacgacg ccaggccata ataatatcga tctcgctggt cgattccggg
      601 ctgacgacac gcaaccgccc ttccgcaata tctttttcga ccatcggata cggcatcgtc
      661 gccacgccaa gccccgccag taatgcctga cgtttatctt caatcgtgct caccgttaag
      721 cgcggctgtt tgtccagcag ctgtacggtc aacaccgggc gctcacgagc ggtatccgct
      781 accgcaattc cacgatattt cacgcgcgtc acttcagata acggttccgg ctcctgatga
      841 atcgggtgat caggcgcggc aacgtagacg ttcattaagg tatagagctt gcgcgagttg
      901 atctccgacg aggaacgaaa atgcatatcc ggcgcgataa cgatatccgc ccgcccctgc
      961 tccagtcgtt cccacgcccc cgccagcact tcggtgatga ttgccagttg ggtattggct
     1021 tttgccgcca gtttgtcgat taacgggaaa aaggcaggtg tcggtaccag cgcctcggtc
     1081 acaatggtga gatgcgtttc ccaaccgcgc gcgagggctt ccgcatcggt agtcagttta
     1141 tctgcggctt ccagcaaaac gcgccctcgc tccagcaaca tcctcccgac attggtgaat
     1201 ttggtgcgat ggcccgagcg gtcaaacagc accacatcca gctcttcttc cagtttttgc
     1261 atggtgtagc taagtgcgga aggcacgcgt cccagctcat ccgccgccgc cgcaaaactg
     1321 ccccggcgat cgatcgcatc cataacccgt agtgcttcca gcgttaatgc cctttctttg
     1381 gccatttcgt tctcattcag gaaatttgaa cataccgggc agaatatctg gctaacaatg
     1441 tagcgtccag ccccttacca taaaaggaag taaagagagg tcaagaatta tgattactac
     1501 ccgaactgcc agacagtgtg gacaagcaga ctacggatgg ttgcaggccc ggtatacttt
     1561 ttcctttgga cactacttcg acccgaaatt gttaggctat gcctccctgc gtgtgcttaa
     1621 ccaggaagtg ctggccccag gtgccgcctt tcagccgcga acctatccca aagtcgatat
     1681 tttaaatgtg attctggatg gagaagcaga gtatcgcgac agcgaaggca atcatgttca
     1741 ggccagcgcc ggtgaggcgt tgctgctctc tacccagccg ggtgtcagct acagcgaaca
     1801 caatctcagc aaagacaaac cgttaacacg aatgcagctt tggctggacg cctgcccgca
     1861 gcgagagaat ccgctgattc aaaaactggc gcttaatatg ggcaagcagc aattaatcgc
     1921 ctcgccagag ggtacgatgg gaagcctgca attacgccag caagtgtggc tgcaccatat
     1981 cgtgctcgac aaaggcgaaa gtgcgaattt ccagttgcat gggccacgcg cgtatttgca
     2041 atcgattcac gggaaatttc atgcgcttac gcatcatgaa gagaaagcgg cgctgacctg
     2101 cggtgatggg gcgtttattc gtgacgaggc taacattacg ctggttgccg attccccact
     2161 gcgcgctttg ctgatagatt tgcctgtcta gttgttttta cagggagatg atgatgagta
     2221 aaaaatcggc caaaaagcgc cagccagtga agcccgtggt ggcgaaagaa cctgctcgca
     2281 ccgccaaaaa ttttggctat gaagagatgt tgagcgagct ggaagccatc gtcgcggatg
     2341 ctgaaacgcg tttagccgag gatgaagcta ccgcgtaaag acaggttgcc ggatgcggcg
     2401 aaaacgcctt atccggccta caaaatccaa caacattaac gaattaaaca acccgtaggc
     2461 cggataagat gcgccagcat cgcatccggc atcaggtaca aaacacaaca acatcaacaa
     2521 gttgagcacc ccgtaggccg gataaggtgc gccagcatcg catccggcac aatactcaaa
     2581 acctggcgtt tatctggcct tgctcgccat aatctcgata atctgccgat ccgtttgctg
     2641 catcgaatgg cttgctaacg cacacaggtt ggcaatcgac tgctcaacat catgcgccac
     2701 gatcccttca ttgccggtca cggcggtatc atccagcgcc attaacaccg ctttccacgc
     2761 agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat cgcaaatcat
     2821 gccgctgaca tcgccgatca tactgctgat cgccatcgag atggtttcat aacgcccatc
     2881 caccagccat gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg cacacagcgc
     2941 agacaaacgc ggtaactggt tatggatgta aattgcgctc aaatgagaaa gcatcagcgc
     3001 acgcgccagc cgttcatcat ccgctccgaa gtgttctgct accaccacca caggcattgt
     3061 tgcggtgatc ccctggttac ccgagccgga gttactcata gccggaagcg tagcgccgcc
     3121 catacgcgca tcggatgccg cgctggtacg aatcacaatg gatgaagaga gatctttcgc
     3181 cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca atatgcagcc cccacttacc
     3241 gctcaaacct tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa taaagcggat
     3301 cgccgcaaac gggacttcat tgacgaactt caggatctca gccagcgtcg ttctggaaag
     3361 caccgtcagc ggagactctt gctcgccctc tgccacacac gcctgctggg taaacaccac
     3421 accattgtgc gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg tgacacacgc
     3481 ccacttctca ccgttccaga ctttggcgcg tgagaagagg atttcattgc aaggttcctg
     3541 gatcttaacg gagactttcc ccgccgccag cagtgctttg gcatcggcaa ttgcctgcgc
     3601 agttgcgtct ttcagcactt ccagcccggc gttggcattt ccacctaacg cccccagcgc
     3661 cgccgcaatc ggcagcccca ccattcccgt gccgggaacg gtgacgccca gaccgttctt
     3721 catcagattt ggcgaaaccc aggcttctac acgttcaacc ggaccttcca gttctgccgc
     3781 agcaaccgcc gccgccagcg ccagtgaaat cggttcagta catcccagcg ccggttttac
     3841 ttcctcctga acggcgagga tgtaacgctg ccataacgga tttaaagtcg aatcaaacat
     3901 attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc aggaacggag aaacacaaag
     3961 caacaaacca gtgacgataa tcaggtacag agacatccct ttgtatttgt gcaatgccgg
     4021 tactttgtaa accagccacg ccgggatcag gcaccctacc atgccgaaaa tcgggctaca
     4081 gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg ccagcaaaat
     4141 ggcgaaaatc atgatgccgc gctgaacaag attttcgtta atcttctcgg caggcatctt
     4201 gcgacgcagg atgttcatta cgatcccttg cgttgcttcg cgaaagccta aatagacgcc
     4261 aaagaatgca gtcatgacag caaagatatt gagaatgacg ctgaccactt tcacccacgc
     4321 tgcgccgtca ccgctaataa actgtgcggc aatcgccagc gcggaaatat tctgctcata
     4381 ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac gacacggcgt agaaaaagac
     4441 ggtgacaaac aaaatgccaa acgcgatatt cattgcccgc aatgctttat gccgcgccac
     4501 ttcaattgat ttttcccgcg agcgataaga gatcaccatc gggcttaacg tctggataaa
     4561 cagaatcgac gtcagggtaa acggcagcgt aataatggcg tttttcacca gcagccccag
     4621 cggcggtagc gaaccgacgt tatacagatg ccacattccc accatcgaca cgcccagcgc
     4681 cgcgaccacc agcagcttgg tcagcaccat gccggtcgaa attttgaata acaatttctc
     4741 gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc agaccataaa agggactgtc
     4801 tgacagcaac ccttccgtca cgccgaaggt atgcagatag gaagcactat cgttggtgat
     4861 ggcggtggaa tagacgaaca tccaaatcac cagcattacg aaatagagcg cacctaacag
     4921 gatgccccag tttttaccta aataaccgct aatgacactc gggtaatctt tacattctgg
     4981 tgattctgcc agcgtattaa taaacaaccg ctgaaacaga tacattgccg ggtaaccaat
     5041 caccgatgag agcaaaaata cccacaatcc catcaaaccg acctgcaccg ggagaaaaac
     5101 aatccccgcg ccaatcgcca tcccgatact cataatcacc cagccggtgt cagtactgtc
     5161 gaatttgatc gcttctcgcc actcgctctc actcattcca gcacgacccg ccggggtcga
     5221 agcgtctgca atgacgcctt tattcgatgc aatttccata atttctcgct caatattttg
     5281 tagggcttat tatttttttc cgagccgcat caaggcgata tgcggtctac gtttttgcag
     5341 gcgagttatt agaataaaga aatgatacgc gcgaggtcag agaaaatctt cacaatcaag
     5401 actttgaaat aacggagatg gataagaatt ttctactaaa ttaatcgcaa gagaatattc
     5461 attctctatt tgcggcagat cacaaaaaaa aggtgcacat ttgtgcaccc aaggatgaaa
     5521 gctgacagca atgtcagccg cagaccactt taatggccag tcctccgcgt gatgtttcgc
     5581 ggtatttatc gttcatatct ttgccggttt catacatcgt ctcgatcact ttatcgagtg
     5641 aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc ggcgtttact gctttcacgg
     5701 cattaatggc attacgttca atgcacggga tttgtacctg tccggcaacc ggatcgcagg
     5761 tcagcccaag gttatgctcc atcgcgattt ccgccgcatt gcatacctgc gccggactgc
     5821 cgcccagtag ttcagttaac cctgccgccg ccattgaaca ggccacgcca atctccccct
     5881 gacagccgac ctccgcgcca gagatggagg cgttcatttt atacagcgcg ccaatagccc
     5941 ccgctgccag aaaatagcgg gcaattgacc gctcgtttac cggacgacgg aacttatcgt
     6001 aataagccag cactgccgga ataatgccgc acgcaccgtt agtcggtgcc gttaccacgc
     6061 gcccgccagc tgcgttttct tcactaaccg ccagcgcgta catgttgatc cagtcgatga
     6121 cattcatcgg atcgttagag atgttatcgc tagaaaccag ctgacgacgc agcgctacgg
     6181 cacgacgcgg cacattgagc ggaccaggca gcacgccttc agtgttcatc ccgcgttcaa
     6241 taccgtcatg catcacttgc cagatacggg caaaccctgc gtcaatttcc gctttactgc
     6301 gcagcgctag ctcgttgtgc atcatcaggc cagatataga caggccgttg taatcacaca
     6361 ttttcagcag ttcacctgct gagtggaaat cgtaaggtac ggacgtttcg acatcgtgcg
     6421 acaggccgaa gtgttcttct tcgacaataa acccgccgcc gacagagtaa taggttttac
     6481 ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat tccgttctca tggcgaggca
     6541 acatttcggg atggaagata atgttctttg ctacaggaaa atcaacaata tgcgcaccag
     6601 atgccactgg cagccgcccg ctgcgcgtta ctaactctat aaatgcaggg atctcatcaa
     6661 tgacaacatc ctgcggactg tttcctgcca atcccatgat aatggcgaca tccgtggcat
     6721 ggcctttgcc cgtcagtgac aacgacccat acagatcgac cacaatatgg ctcgtcgcgg
     6781 ttaataagcc gctactttcc agccgatcaa taaaactttt tccggcattc attggcccca
     6841 cggtatgcga actggaggga ccaatcccaa ttttgaaaat atcgaatgca ctaatcatat
     6901 ccacaccctc ggattgccgt tcagtgaagt ggagcggaac gaccttgcga ccgtcccgct
     6961 cacgaggctt tacgcactac gtactgcgat agcttcaatt tccagcttca catctttcgg
     7021 caaacgcgca acctgcacac agctccgggt cgggtaggtc gcctgatgct catcgaagaa
     7081 ctgcttatag acttcattga tggtggcaaa atcattcaga tcggtgatga acacggtcat
     7141 cttgatgata tcgcccacgc tcagcccggc agcaaccacg atcgctttga cgttttcgag
     7201 gcttaaacgc gcctgatctt gcacatcagc cgggatctca ccggtctgcg ggcaaaccgg
     7261 aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg ccctgaacat aagggccgat
     7321 tgcgcctggg gcacgttgcg tttcgataat ctttttcata catcctccgg cgtcagagcg
     7381 cctgggtaaa agtacgtgaa ataacatcct gttgctgttc acgggtcagt gcgttgaagc
     7441 gcacggcgta gccagagaca cggattgtca ggttaggata tttttccggg tgctcgatgg
     7501 catccagcag catttcccga ttcattacgt tgacgttgag gtgttgaccg ccttcgacat
     7561 ccgcttcgtg gtggaaatac ccatccagca ggccgacaag gttggtttta cgtactggat
     7621 cttctttgcc cagcgccgca ggaacgattg agaaggtgta cgagatccca tctttggcgt
     7681 aggtgaacgg cagtttcgcc accgacgtca atgaggccac ggcacctttg cggtcacgac
     7741 catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc gcgacgaccg tccggcgtat
     7801 taccggtttt ctggccgtac accacgttag aagtgatagt cagaatcgac tgggtaggga
     7861 cggcgttgcg ataggttggc agcgctttaa ttttcttcat aaagcgttca accaggtcgc
     7921 aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg cggatattca ccgtcgattt
     7981 caaagtccac cgccaggccg ttttcgtcac ggattggttt cacgcgggca tatttgatgg
     8041 cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc gcatgccata gtgcgataga
     8101 catcacgatc gtgcagcgcc atcagcgaag cttcgtagct gtacttgtcg tgcatgtagt
     8161 gaatgatatt cagcgcgctg atgtactgca ccgccagcca gtccatgaag tgatcgaggc
     8221 tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag cggtgctgtt ttcggcccga
     8281 cctgaatctt cagcttctcg tccaccccgc cgttaattgc gtagagcagc gttttcgcca
     8341 ggttagcgcg tgcaccaaag aactgcattt gcttaccaat caccattggg ctgacgcagc
     8401 aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat cagatcgtca ttttcatact
     8461 gcaaggaaga ggtgacgatc gacacctgcg cggcatattt tttgaaggca atcggtaatt
     8521 cttccgacca aagaatggtc aggttaggtt ccggtgccgg ccccatagtg tgcagggtgt
     8581 gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc gtccagcccc atcccgccga
     8641 tcacttccgt cgcccagatt gggtcgccgg agaacagcga atcaaattcc ggtgtacgca
     8701 ggaagcgtac catacggatc ttcatgatga agtgatcgat cagttcctgt gcctgctgct
     8761 cattgagtac gccagcttta aagtcgcgct caatgtagat atcgaggaac gatgccgtgc
     8821 ggcccagcga catcgcgccg ccattttgcg atttcactgc cgccagataa gcgaagtaga
     8881 gccactgcac cgcttcctgc gcattctgcg ccgggcgaga gatatcaaag ccatatttcg
     8941 ccgccatttc ctgaatctgc aacagcgcat gacgatgctc tgccagctcc tcacgcagac
     9001 ggatggtggc ttccagatca tcgccttttt ccagacgaga ctggagatcg gcaaattgca
     9061 gttcgcgttc acgtaccaga taactgatgc catacagcgc tacgcggcga tagtcaccga
     9121 taatgcgccc acggccatag ccatctggta aaccggtcag cacgccagat ttacggcagc
     9181 gcagcatatc cggtgagtaa acatcaaata cgccctggtt atgggtttta cgcagatcgg
     9241 taaacagata ttcaaattca ctgtccattt ctcggccata ggcgtggaat gaacttttaa
     9301 tcatattaat gccaccgaac gggtgtagcg cacgtttcaa cggcgcatcc gtttgcaggc
     9361 caacaatttt ttccagcggc tggttaatat atcccgcatc atgagcggta attgtggtgg
     9421 caatattggt atcgaaatca accggcgcgt gggttgcatt ttcgatacgg atgccttcca
     9481 ttactttttc ccacaattcc gtggtggcag gcgtcgcttc ggcgaggaaa gattcatcgc
     9541 cttcatacgg tgtatagtta tgttgaataa aatcgcggac attaatttcg tttttccagt
     9601 ccgtaccttt aaagccaagc catgcgtcgg cgtacagctt atcgctggta tcaatatcta
     9661 ccttcatgaa aaataatctc tctacaatac ttcaactaaa ttatgcaaat tctgcgggcg
     9721 cgttaacttt gcctaaatga atggcatcca aagcaatcat tttttcttcg ttagtcggaa
     9781 taacggcaca aatgacacgc gcattttcac tggaaacaat tcgctctcca aaagagttag
     9841 atcgattatt catttctgta tcaatcacta cgcctaatac agccaaatgt tccatgacca
     9901 gacgacgaat taaacttgaa ttctctccta ttccgccggt gaatataatt ccatccaggc
     9961 gacgtaatga agctgcgtgt ccggcaatat gacgggcaat tcggtgaaca aaggttttaa
    10021 ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt ttccagaaca cgtaaatccg
    10081 aagaaagacc ggaaatacct aataatcccg actctttatt cactacgcgt tccaggtcac
    10141 ccagactctg gttggtttgg ctggcgaccc aggacatcgc accaaagtcg acatcgccac
    10201 tgcgggtacc catcatcaag ccttccagcg gcgtcattcc cattgaagta tcaacactct
    10261 gaccgttgcg aaccgcgcag attgacgcgc cattgccaag atgcgccaca accaggccgg
    10321 agtcatcttc cgccagattc agcagcgaat gtgcgcgctg ggaaacatag cggtgcgacg
    10381 tgccgtggaa accataacgg cgtacaccta actcttcata atatttccac ggcaggccgt
    10441 ataaataagc ttccggagcc atcgtctggt ggaaactggt atcaaatacc gccacctgag
    10501 ttacgcccgg aaataattgc tgcgccgatt caataccact taaattggcg taattatgca
    10561 ggggtgccag tggagaaacg cgacggatat tatcaatgac ttcatcggta ataatggcgg
    10621 actcggtaaa aatactgccg ccgtgagcga tgcggtggcc aattaaggcc acactgtcat
    10681 ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa tgcaccttcg tagctgtggt
    10741 gagccagcgg tgctggctct cccccattta cggataagaa tgcattttcc gagttaatac
    10801 cgtcggcaat acctgacatt aatacttcac agtcgctggc atcgagcacg gaaaacttaa
    10861 tcgaagacga accacagtta ataaccaaaa caaccggaaa ttcattcatc tcttttctca
    10921 tcctgagtta cggattaaaa cagtttgtat acgatgttca ggatggtcag cagaccaatc
    10981 acggtaacaa acacgttatc cagacgacca cggtatttcg ccagagacgg cgctttacgg
    11041 atggcataca tcggcaacag gcacagcagg gatgcgataa tcggtgcgcc catggcttca
    11101 atcaggtcaa ggatgttcgg gttggcgtag gcaacaaccc aggtggagcc catgatgaag
    11161 atcatgctga tagtgttcag tttacccagc gacactttag ttttgtcgcc tttataacca
    11221 aacttcagga ccaggccatt cagaccttcc agcgttccca gatagtgacc gaagaaagat
    11281 ttgaagatag ccacgagtgc gatgatggaa gccgcatatt ccagtgtaat cgcgaacgtt
    11341 gttttggtac cggtcatgga cgcaaagtgg ttagccagat aagaaagcac tggaatattc
    11401 tgcgctttgg cttccgccat gttggccgga gacagagtaa acaggcagct aaaggcaaag
    11461 aacatcacca ctgcaaccat cagcatgctg gcacgagaaa tgatttggga acatttacgt
    11521 tcggtgaagt cgcgaccgaa gtctttctca tactcttcac gcttagaaac cacgaaggaa
    11581 gagacgattg gcgagaagtt aaaggagaaa accatgatgg aaatccccag ccagacagtg
    11641 atcaggatac cgtcatgacc ggttaacgac agcgaaccga ggtcaacctg gtcgataact
    11701 gcagagttcc agtaagggat cagcgacaaa gaaatcagca ccaggctggc gataaacggc
    11761 cataccaggt agctcatcac tttaaccatc agatccttac caaaccagat gacgaaagcc
    11821 atcagcagca acaggaacag cgccacaaag ccgcgattca gcggtgcaaa gccgagctgg
    11881 ttttcccaga acgtcataaa ggtattggta atagtaacgc cataaatcca cagcagtggg
    11941 caaatcgcga agaagtacag gaacgtgata accacgccgc cagttttacc aaaatgctct
    12001 tccaccgttt ccgtaatgtt gccggaaggg ttagagccag aaagacacag acgcgccagc
    12061 gcccggtggc aataaaacgc gatggggtat gccaatacca acatcagaag aatcgggatc
    12121 agtccgccaa aacctgcgcg gatagggaag aacagcaccc cggcgccgat tgccgtacca
    12181 aacaagccta acgtccatgt ggtatctgat ttacgccagg acgattgttt tgtctggctg
    12241 gatacaatgc tatctgaagt actcatatcc tatcctcaac gaattaatta agcgtcaacg
    12301 aaaccggtga tttgagagac gcgagaaaga tcgatattgc cgccggaaat aatactgacg
    12361 gtttttctgt tttgaatata ttggtctaat ttaccgctta ataatgcagc acatgccaga
    12421 gcgcctgcgc cttcggtgac gactttattg cgctgaatta aggcaatcat actgtttctg
    12481 atttcgtctt cgctgaccag cacgatgtca tcgactaatt cacgaacgat ttcgtaagtt
    12541 aaattacccg ggcgggagac atcacaacca tccgccaggg tgccggtagt tcggtgcgtg
    12601 gttatttctc cggagtggaa agaagccgcc atgccgtgaa cgttttcaga ctgtacgcca
    12661 ataacacgaa tggtcgggtt aatagattta attgccaccg caataccagc aattaaaccg
    12721 ccaccaccaa ttggcacaat cacgttatcg acatcataga gatcttccat aatttccaga
    12781 ccaatcgttc cctggccagc aatcactttc ggatcatcgt aaggtgggat aaaaatacgg
    12841 ccttccattt cgacaatttc gctcacttta gcgatagtgt cgttgaagtt atcaccatgc
    12901 agaacgactt ctgcggagta gtcgcacgtt gccgctactt tggattttgg cgcacctttt
    12961 ggcatcacca ctttaccgtc gatacccagc atcgcgcagg acagggaaac cccttgcgca
    13021 tggttgcccg cagaacaggc caccacgcct ttgcgttttt ccgcatcggt cagtgaactt
    13081 aatttattaa atgcgccacg aattttaaat gaacccgtac gctgcatatt ttcaaacttc
    13141 aggaatattt cacctttgca acgttcacta aaatagttgg agcgaggcat gcctgtttta
    13201 taaattcgcc cagccagtcg ttgtttcgct tcaataatgt catcaatagc aaccggcaga
    13261 tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt aaatatgtag gtaaccgtaa
    13321 ccgacacctg caa
//
LOCUS       DAPHJE010000115        13316 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPHJE010000115 DAPHJE010000000
VERSION     DAPHJE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.116"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..433
                     /locus_tag="RH222_003556"
     CDS             <1..433
                     /locus_tag="RH222_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV3798518.1"
                     /translation="RISPEYKIIVPMFLHIWLSSPVMQTWLVQSSKEVARKTLNLKDL
                     KNAFVPLPSIEEQHEIVRRVEQLFAYADSIEKQVNNALARVNNLTQSILAKAFRGELT
                     AQWRAENPDLISGENSAAALLEKIKAERAASGGKKASRKKF"
     gene            661..1002
                     /gene="symE"
                     /locus_tag="RH222_003557"
     CDS             661..1002
                     /gene="symE"
                     /locus_tag="RH222_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDV3798519.1"
                     /translation="MTDMHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFTTGTAVDVKVMEGCIVLTAQPLAVEESELMQSLRQVCKLSARKQKQVQAF
                     IGVIAGKQKVA"
     gene            1164..2543
                     /gene="mcrB"
                     /locus_tag="RH222_003558"
     CDS             1164..2543
                     /gene="mcrB"
                     /locus_tag="RH222_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease subunit McrB"
                     /protein_id="HDV3798520.1"
                     /translation="MESIQAWIEKFIEQAQQKSSQSTKDYPTSYRNLRVKVSFGYGNF
                     TSIPWFAFLGEGQEVSNGIYPVILYYKDFDELVLAYGISDTNKPHAQWQFSSDIPKTI
                     AEYFQTTSGVYPKKYGQSYYACSQKVSQGLDYTRFASMLDNIINDYKLIFNSGKSVIP
                     PMSKTESYCLEDALNDLFIPETTIETILKRLTIKKNIILQGPPGVGKTFVARRLAYLL
                     TGEKAPQRVNMVQFHQSYSYEDFIQGYRPNGVGFRRKDGIFYNFCQQAKEQPEKKYVF
                     IIDEINRANLSKVFGEVMMLMEHDKRGENWSVPLTYSENDEERFYVPENVYIIGLMNT
                     ADRSLAVVDYALRRRFSFIDIEPGFDTPQFRNFLLNKKAEPSFVESLCQKMNELNQEI
                     SKEATILGKGFRIGHSYFCSGLEDGTSPDTQWLKEIVMTDIAPLLEEYFFDDPYKQQI
                     WADKLLGDS"
     gene            2543..3589
                     /gene="mcrC"
                     /locus_tag="RH222_003559"
     CDS             2543..3589
                     /gene="mcrC"
                     /locus_tag="RH222_003559"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methylcytosine-specific restriction
                     endonuclease system specificity protein McrC"
                     /protein_id="HDV3798521.1"
                     /translation="MEQPVIPVRNIYYMLTYAWGYLQEIKQADLEAIPGNNLLDILGY
                     VLNKGVLQLSRRGLELDYNPNTEIIPGIKGRIEFAKTIRGFHLNHGKTVSTFDLLNED
                     TLANRIIKSTLAMLIKHEKLNSTIRDEARSLYRKLPGISTLHLTPQHFSYLNGGKNTR
                     YYKFVISVCKFIVNNSIPGQNKGHYRFYDFERNEKEMSLLYQKFLFEFCRRELTSANT
                     TRSYLKWDASSISDQSLNLLPRMETDITIRSSEKILIVDAKYYKSIFSRRMGSEKFHS
                     QNLYQLMNYLWSLKPENGENIGGLLIYPHVDTAVKHRYKINGFDIGLCTVNLGQEWPC
                     IHQELLAIFDEYLK"
     gene            complement(3646..9990)
                     /locus_tag="RH222_003560"
     CDS             complement(3646..9990)
                     /locus_tag="RH222_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3798522.1"
                     /translation="MTTRYFSSLIEQSLSRSTEATLSIMGVTNPQLREHLAQQMGADC
                     GKPGSFLASPVFQQMFGWKESNHTMRSLTEGNALLSKAVVDSLDDQSNGRYRFGADWK
                     PFTHQLASWKALLEKKHSVVVTSGTGSGKTECFMVPVLEDLYRELHENGNNPLVGVRA
                     LFLYPLNALINSQRERLDAWTRGFGTGIRYCLYNGNTEELHAKMKTVQDNRPNEVLSR
                     EKMRLEPAPILVTNGTMLEYMMVRQVDAPIIQQSKAQKSLRWIVLDEAHTYVGSQAAE
                     LALQLRRVMTAFGVTPDDVRFVATSATIACSDAEKQLKKFLSELSGIPQERIDVLDGS
                     RVIPELEPSKNVSVPLDEIEQIPDLDLKDKNDNKIKGISPERFYALTHSPEARYIREM
                     LVSQPNPMKLDEMTQRLNALTKQHYSQQEVLRWIDVCSGTQPNAKDPAFLKVRAHIFQ
                     RNTQGIWACVDKDCRLKHGTPLEKGWPFGYVYVNQRQNCDCGSPVYEVAFCNDCNEPH
                     LLAQDKKGKLVQWENKGGDEFSLQDEVPVEHDTTEEKVEKENGFLPPLIIAAGETSET
                     GYTLQRLDHQTRRIGVINNDSIPLIINDVEQVCSASGCGYRGMSGKQPFRRALLGGPF
                     YVTNIVPTVLEYCQDFTSDEGKEGVGPDSLPGRGRRLITFTDSRQGTARMAVRMQQEA
                     ERSLLRGSVVEILSWHQRTQTSTAPNANADLEKLAARAKQAREQAEEYRSWGMPDQAK
                     LSQAQAEQLEQAYQAATGGKAATTLVSRTWTEMVNELKDKADIRGPVLKYNHYLQPEV
                     FNKNGGPLKLSEMLLFREFMRRPKRTNSLETQGLVQVGYQGLEKIHKSPLHWQEKGLT
                     LDDWRDFLKVTLDLYVRESNFTQLDDELKNWIGSRFSSKFVRNPESKDPEDNQNRRWP
                     QIRNGNVSHRLAKLLMLGAGFKTVNTATIDIINTWLKEAWAQLTGPLAVLKPDGNRFY
                     LPKEHMTFSLITDAWICPVTNKILDTAFKGLTPYLPTHISFEHLTQAQYDTFVAQKVT
                     MPEIWKLDRSQEDYAEGLAKARDWVNNDPLIAQLRSENVWTDINDRVVEGGFYYRTAE
                     HSAQQSSERLQSYEKMFKNGQLNVLNCSTTMEMGVDIGGITAVVMNNVPPHPANYLQR
                     AGRAGRSKESRAISYTLCKGNPHDQQVFANPLWPFETMIPAPMVAMNSARLVQRHVNA
                     LLLSDFLCNVIGETDKEKTSLDSLWFFGEDDGQSKCERFKVWLERPVLDIDTALERLV
                     KGTALHGARAEHLRDKTINAITFLQQRWLSVYRDLVTQERESQPQTPYRKRIELEKKR
                     HCGEYLLRDLAARTFLPGYGFPTDVVTFDNFTMEDYIREKSQKSRDKKDREDNVSRYK
                     GLPSRNLGVAIREYAPGAEIILDGRVFRSAGVSLHWHNINADTNEAQRLDCAWRCHKC
                     GTIGYEEGMSSSGMLFCSNSACGEKITMDNRRQVLQPAGFVTDAHAPVTNNIETMKFV
                     PVVPAWVFVKAEPVPLPNPLMGYMASGADGHVFQQSLGEGGHGYALCLSCGRAESMLN
                     ENDAPKSMEAHYPPRPGKADRDSQNHRLICPGSTALMKNVTLGALARTDVFEMVLRKP
                     QNGEYLPDNTEEGRIVAMTLAVALRQALAGVLGISAAELGYSVRPVRLEDGQSVLAVQ
                     LYDVISGGAGFASSAPVHIEAILQGMVKQLGCRHCDTACSECLLDSQTRHDHDLLDRK
                     VALAWLGDDFTYYIGLPDEETFSLPDARYCPGAIGDTIRRAINEGAEKLTLWMTGAPN
                     EWDLYARQFRAAIQSYRLKDNVEVDLVIPAGVDDPDLLHELSQFTALGVRLCHVEQEL
                     QLPIVAQVTFADRVMTLASRSQQATIPGPEWHLNDELVVRSLGYQTVELNEFILPAKA
                     ANAVERVKDIQIHKQLNGPLSQFGQRFWDVLFNDHEEAQSLMKNTRITGVHYTDRYLQ
                     NPVALALLGSILKPLKTKLTDGAEVALDTLFKDKDRPGNRPFHDWMSIADFQDFADQW
                     FAAALGRPIELTVFDSPRDIPHHRKLTVTFEDGQVLKIRFDQGMGYWRINFASQWHYF
                     DFRDDVSFQLVKMAQACKEGNVANSEESWATDVLVEVIAS"
     gene            complement(10003..13293)
                     /locus_tag="RH222_003561"
     CDS             complement(10003..13293)
                     /locus_tag="RH222_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798523.1"
                     /translation="MGQSEYISWVKCTSWLSNFVNLRGLRQPDGRPLYEYHATNAEYN
                     QLTQLLCAVGQSQSNICNRDFAACFVLFCSEWYRRDYERQCGWTWDPIYKKIGISFTA
                     TELGTIVPKGMEDYWLRPIRFYESERRNFLGTLFSEGGLPFRLLKESDSRFLAVFSRI
                     LGQYEQAKQSGFSALSLARAVIEKSALPTVFSEDTSVELISHMADNLNSLVLTHNLTN
                     HKEPVQQLDKVHPTWRSEFPIPLDDETGTHFLNGLLCAASVEAKPRLQKNKSTRCQFY
                     WSEKHPDELRVIVSLPDEVSFPVTSEPSTTRFELAIYEDGEEVSGLGPAYASLENRQA
                     TVRLRKSEVRFVRQNPSAGLSLVARAGGMIVGSIKLDDSEIAIGEVPLTFIVDADQWL
                     LQGQASCSVRSSDVLIVLPRDNSTVAGFDGQSSAVNVLGLKALPVKGCQDVTVTANET
                     YRIRTGREQISIGRFALNGKRASWVCHPDETFIGVPKVISTLPDIQSIDVTRYLSGIS
                     IEQCHIQEMLGAQYLSIRNSNNETLLRRKIGILPADFSIEIKGGMHANEGTIVITTQH
                     PCVATLKDKTLEATRKRTEGRTEIQLKAEGVPPAFITLQVLPNLAADTIDIELPFPAK
                     GCLALDVNGRPLDKNITLHDLLGSRAFLFSRNGEPTKYTLQLHLRSISGLQAWHEWSY
                     TALSERPVELNLYSLREHIENLISLEAGIDQVVEMRITGAGVVMAWQIRRYKYSLRYD
                     YEKELLLSQSVNHRAGQIPSPVIMLLSEPERKSIPLASRMSEGVPVGEYELSSIVNKN
                     GPWLVVPKPGEEMAFRPCFIRGESSLPVEESNIRSLQKATQLFNPQAEVNTITRVLGQ
                     MANDPAHSGWQFMRSLYDQFGYLPLATFEVWRALVQHPQALAMSLFKFEMSAEYLSRI
                     ENEFPILWEFFPIFEIKAASERFKLFLSQKGAPEETQKLLVTNMFQRLGLVFPTYADE
                     IEKWLSNGYLPPSIPESCVHGWYQELLREHSEARWPEYGCKRLYKWMMSQKNPVIGIN
                     PDANHRYSVAWLPVFAAAVASGNASFESVFDRKPGAVFFLRQVRDFDSRWFKAIFQFS
                     LLRYVAKK"
BASE COUNT         3498 a         3305 c         2962 g         3551 t
ORIGIN      
        1 cagaatatct cctgaatata agattattgt tcctatgttt ctccatatat ggttaagttc
       61 accagttatg caaacatggc tagttcagtc atctaaggag gtcgccagaa agacattaaa
      121 tttaaaagat cttaaaaatg catttgtccc tttaccgtct atagaagaac aacacgaaat
      181 cgttcgccgc gtcgagcaac tcttcgccta cgccgactcc atagaaaaac aggtcaacaa
      241 cgccttagcc cgcgtcaaca acctgacgca atccatcctg gcaaaagcgt tccgtggtga
      301 actcaccgcc cagtggcggg ccgaaaaccc ggatttaatc agcggagaaa acagcgccgc
      361 tgcgttgctg gagaaaatca aagctgagcg cgcagccagc gggggtaaaa aagcctcgcg
      421 taaaaagttc tgatcattat tttctggcgc acctttccgg tgcgcttttt attatttctc
      481 gccaatcata actcacataa atatatttaa atcattccgg aaattgccca ttttattcta
      541 tttttagctg gactttccct gtatttactg atgatatatc caggtattta gcgcggtgcg
      601 gatgtgcgcc aacacacccg caccgctaat cacaatccct atttctaagg aatagtaatc
      661 atgactgaca tgcattctat tgcacaaccg ttcgaagcag aagtctcccc ggcaaataac
      721 cgtcaattaa ccgtgagtta tgcgagtcgc tacccggatt acagccgtat tcccgccatc
      781 accctgaaag gtcagtggct ggaagccgcc ggttttacca ccggcacggc ggtagatgtc
      841 aaagtgatgg aaggctgtat tgtcctcacc gcccaaccac tcgccgtcga agagagcgaa
      901 ctgatgcagt cgctgcgcca ggtgtgcaag ctgtcggcac gtaaacaaaa gcaggtgcag
      961 gcgtttattg gagtgattgc cggtaaacag aaagtcgcgt aacagcgttt actcccggtt
     1021 aaccaccggg agccttccac tgactcaata gaaactttcc ccctcagtaa atatttaccc
     1081 accacctttt gcagtaaaaa tctattgttt cagtacgttg cgaaagcgat aatagaggct
     1141 tagcaatgag gaaggcacat cttatggaat caattcaagc ctggattgaa aaatttattg
     1201 agcaagcaca gcaaaaaagt tcacaatcca ccaaagatta tccaacgtct taccgtaacc
     1261 tgcgagtaaa agtgagtttc ggttatggca attttacatc tattccctgg tttgcatttc
     1321 tgggagaagg tcaggaagtt tctaacggta tatatcccgt tattctctat tataaagatt
     1381 ttgatgagtt ggttttggct tatggtataa gcgacacgaa taaaccacat gcccaatggc
     1441 agttctcttc agacatacct aaaacaatcg cagagtattt ccagacaact tcaggtgttt
     1501 atcctaaaaa atacggacag tcctattacg cctgttccca aaaagtctca cagggtcttg
     1561 attatacccg gtttgcctcc atgctggaca acataatcaa cgactataaa ttaatattta
     1621 attctggcaa gagtgttatt ccacctatgt caaaaactga atcatactgt ctggaagatg
     1681 cgttaaatga tttgtttatc cctgaaacca cgatagagac gatactcaaa cgattaacca
     1741 tcaaaaaaaa tattatcctc caggggccgc ccggcgttgg aaaaaccttt gttgcacgcc
     1801 gtctggctta cctgctgaca ggagaaaagg ctccgcaacg cgtcaatatg gttcagttcc
     1861 atcaatctta tagctatgag gattttatac agggctatcg tccgaatggc gtcggcttcc
     1921 gacgtaaaga cggcatattt tacaattttt gtcagcaagc taaagagcag ccagagaaaa
     1981 agtatgtttt tattatagat gaaatcaatc gtgccaatct cagtaaagta tttggcgaag
     2041 tgatgatgtt aatggaacat gataaacgag gtgaaaactg gtctgttccc ttaacctatt
     2101 ccgaaaacga tgaagaacga ttctatgtcc cggagaatgt ttatattatc ggtttaatga
     2161 atactgccga tcgctctctg gccgttgttg attatgccct gcgcagacga ttttctttca
     2221 tagatattga gcctggtttt gatacaccac agttccgtaa ttttttactg aataaaaaag
     2281 cagaaccttc atttgttgag tctttatgcc aaaaaatgaa tgagttaaac caggaaatca
     2341 gcaaagaggc cactatcctt gggaaaggat tccgcattgg gcatagttac ttctgctccg
     2401 ggttggaaga tggcacctct cctgatacgc aatggcttaa ggaaattgtg atgacggata
     2461 tcgccccttt actcgaagaa tatttctttg atgaccccta taaacaacag atatgggccg
     2521 acaaattatt aggtgactca tagtggaaca gcccgtgata cctgtccgta atatctatta
     2581 catgcttacc tatgcatggg gttatttaca ggaaattaag caggccgacc ttgaagccat
     2641 tcccggtaac aatcttcttg atatcctggg gtatgtatta aataaagggg ttttacagct
     2701 ttcacgccga gggcttgagc ttgattacaa tcctaatacc gagatcattc ctggcatcaa
     2761 agggcgaata gagtttgcta aaacaatacg cggcttccat cttaatcatg ggaaaaccgt
     2821 cagtactttt gatctgctta atgaagatac gctggctaac cgaattataa aaagcacatt
     2881 agccatgtta attaagcatg aaaagttaaa ctcaaccatc agagatgaag ctcgttcact
     2941 ttatagaaaa ttaccgggca ttagcactct tcatttaact ccgcagcatt tcagctatct
     3001 gaatggcgga aagaacacgc gttattataa atttgttatc agcgtctgta agttcatcgt
     3061 caataattct atcccaggtc aaaacaaagg acactaccgt ttctatgatt ttgaaagaaa
     3121 cgaaaaagag atgtcattac tttatcaaaa gtttcttttt gaattttgcc gccgtgaatt
     3181 aacgtctgca aatacaaccc gctcttattt aaaatgggat gcatcgagca tatcggatca
     3241 gtcacttaat ttgttacctc gaatggaaac tgacatcacc attcgctcat cagaaaaaat
     3301 acttatcgtt gacgccaaat actataagag cattttttca cgacgaatgg gctcagaaaa
     3361 atttcactct caaaatcttt atcaactgat gaattactta tggtcgttaa aacctgaaaa
     3421 tggcgaaaac atagggggtt tattaatata cccccacgta gacaccgcag tgaaacatcg
     3481 ttataaaatt aatggcttcg atattggcct gtgtaccgtc aatttaggtc aggaatggcc
     3541 gtgtatacat caagaattac tcgccatttt cgatgaatat ctcaaataag cggtaagttt
     3601 tatagacaat accgctcccg acacgacgtc gggagcggca catcatcagg aggcgatcac
     3661 ctccaccagc acatccgttg cccagctctc ttcgctgttg gccacattcc cttccttgca
     3721 ggcctgagcc attttgacta actggaaaga aacgtcatcg cggaaatcaa agtaatgcca
     3781 ttgcgatgca aagttgatgc gccagtagcc catcccctga tcgaagcgga tcttcaatac
     3841 ctgaccatcc tcaaaagtca ccgtcagttt acgatggtga gggatatcgc gcggcgaatc
     3901 gaacaccgtc agttctatcg gcctgcccag cgcagcagca aaccactgat cagcgaaatc
     3961 ctgaaaatcc gctatgctca tccagtcatg gaaaggccgg ttaccggggc gatctttatc
     4021 tttaaacagc gtatccagcg ccacttcagc gccatcggtc agctttgttt ttagcggttt
     4081 cagaattgat cccaacaacg ccagcgcaac cgggttttgc agatagcgat cggtatagtg
     4141 aacgccggta attcgcgtgt ttttcatcag tgactgcgct tcttcatggt cgttaaacag
     4201 cacgtcccag aagcgttgcc caaactgcga aagtggtcca ttcaactgtt tgtggatctg
     4261 aatgtctttg acccgctcga cagcgttggc ggctttagcg ggcagaataa actcgttcag
     4321 ttctaccgtt tgataaccga gactacgcac caccagttcg tcattcaggt gccactccgg
     4381 ccccggaatc gttgcctgct gactgcgaga ggccagcgtc atcacgcgat cggcaaaggt
     4441 gacctgggca acaatcggta attgcagctc ctgctcaaca tggcacaacc gcacacccag
     4501 cgcagtaaat tgcgatagtt catgcaacag gtctgggtca tccactcccg caggaataac
     4561 aagatcgacc tcgacgttgt cttttagccg atagctctga atagccgcgc ggaactgacg
     4621 ggcgtataaa tcccactcat tgggcgcgcc agtcatccac agggtgagct tttcagcgcc
     4681 ttcgttaatc gcccggcgaa tcgtatcgcc aatagcgcca gggcagtagc gggcatccgg
     4741 cagcgagaat gtctcttcat ccggtaaacc gatgtaatac gtgaagtcat cgccaagcca
     4801 agccagcgcc actttgcgat ccagcaaatc gtgatcgtgg cgagtctggg aatcaagcag
     4861 acattcactg catgcagtat cacaatggcg gcagcctaac tgtttcacca ttccctgcaa
     4921 tatagcttca atatgtaccg gtgcgctgga ggcaaaacca gcaccaccgc tgatcacatc
     4981 ataaagctga acggctaata ccgattgccc atcctccagt ctgaccggac gtacagaata
     5041 gccgagttct gcggcggaga tacccagaac gcctgccagc gcctgacgca gagcaaccgc
     5101 cagagtcata gcgacaatcc gcccctcttc tgtgttatct ggcagatact cgccgttttg
     5161 cggtttgcgc agaaccattt caaagacatc ggtgcgcgcc agtgcgccca gggtgacgtt
     5221 cttcatgagt gccgtcgaac cggggcaaat cagacgatga ttctggctgt cgcggtccgc
     5281 tttacccgga cgcggtggat agtgagcttc catacttttc ggtgcatcat tttcattcag
     5341 catcgactcc gcacggccac agctcagaca caatgcataa ccgtgtccac cttcccccaa
     5401 actttgctgg aaaacatgcc catcagcccc agaagccata taacccatta gcgggttagg
     5461 caacggtacg ggctctgctt taacaaagac ccacgcgggg acaaccggga cgaatttcat
     5521 ggtttcaata ttgttcgtta ccggagcgtg tgcatcggtg acaaacccgg caggctgcaa
     5581 cacctgacgg cgattatcca tggttatttt ttcgccgcag gcgctgttgg agcaaaagag
     5641 catcccggaa ctgctcatcc cctcttcata gccaatggtc ccgcatttgt gacagcgcca
     5701 ggcgcaatcc agacgctggg cttcattggt atcagcatta atgttgtgcc agtgcagtga
     5761 gacaccggca gagcggaaga cacgaccatc aagaatgatc tctgctcccg gtgcatattc
     5821 acgaatggca acaccgaggt tacgggaagg taatccttta taacgggaga cgttatcttc
     5881 acggtccttt ttatcacgac ttttctgcga tttttcgcgg atataatctt ccatcgtgaa
     5941 gttatcaaac gtcactacat ctgtcgggaa accgtatccc ggcaggaagg ttctggcagc
     6001 cagatcgcgc agcagatact cgccacagtg ccgtttcttc tccagctcta tgcgcttacg
     6061 ataaggcgtt tgcggctgcg actcacgctc ctgggtcacg agatcgcgat aaacggagag
     6121 ccaccgctgt tgcaggaagg tgatcgcatt gattgtttta tcccgcagat gttcggctct
     6181 tgcgccatgc aacgccgtac ctttcaccag ccgttccagc gccgtgtcga tatcaagaac
     6241 cgggcgttcc agccagactt taaagcgttc gcattttgac tgcccgtcat cttcaccgaa
     6301 aaaccagagg ctatcgaggc tggttttctc tttatcggtt tcgccaataa cgttacataa
     6361 aaagtcagac agtaataatg cattgacgtg acgttgcacc agtcgggcgg agttcatcgc
     6421 gaccatgggt gccgggatca tggtttcaaa tggccagagt gggttggcaa aaacctgctg
     6481 gtcgtgtggg ttacctttgc atagggtata agagatcgcg cgtgattctt tactacgccc
     6541 ggctcgcccg gcgcgctgta aatagttggc cggatgtggc ggaacgttgt tcattaccac
     6601 cgcagtaatg cccccaatat caacccccat ttccatggtg gtggaacagt tgagaacatt
     6661 tagttgcccg ttcttgaaca tcttttcata gctttgcagg cgttctgacg actgctgggc
     6721 agagtgctcg gcagtgcgat agtagaaacc gccttccact acacgatcgt tgatgtctgt
     6781 ccagacgttt tccgaccgca attgtgcgat cagcggatca ttgttgaccc agtctcgtgc
     6841 ttttgccaga ccctctgcgt agtcttcctg tgaacgatcc agcttccaga tttccggcat
     6901 ggtgactttc tgcgcgacaa aagtgtcata ttgcgcctgg gtaagatgct cgaacgaaat
     6961 atgggtgggc agataagggg ttaagccttt aaaagccgta tccaggattt tattggttac
     7021 cgggcaaatc caggcatccg tgattaaaga aaaagtcata tgctctttcg gtaaataaaa
     7081 acggttgcca tcgggtttca gtactgcaag tggtccggta agttgggccc atgcttcttt
     7141 cagccatgta ttgataatat caatagtcgc cgtattgacg gttttgaatc cagcccccag
     7201 catcagtaac ttcgctaaac gatgggatac gttgccatta cgaatttgag gccagcgtct
     7261 gttctgatta tcttcaggat cttttgattc cgggttacgg acgaattttg atgaaaaacg
     7321 gctaccaatc cagtttttca gctcatcatc cagctgtgtg aagttgcttt cgcgaacata
     7381 aagatccaac gtaaccttga gaaaatcgcg ccagtcatcc agcgttaatc ctttttcctg
     7441 ccagtgcaag gggctcttat gtattttctc cagcccctgg taaccaacct gaaccagccc
     7501 ctgtgtttcc aggctgttag tccgtttagg gcgacgcatg aattcccgga acaacaacat
     7561 ttccgaaagc tttagtggcc cgccgttttt attaaacact tcaggctgca aatagtggtt
     7621 atatttcagt accgggccac ggatatcggc tttatcttta agctcgttaa ccatttctgt
     7681 ccaggtgcgg gataccaggg tagttgctgc ttttccaccg gttgcagcct gataagcctg
     7741 ttcaagctgt tcagcctgtg cttgtgacaa tttcgcctgg tctggcattc cccagcttcg
     7801 atattcttct gcctgctcac gggcttgctt tgccctggcc gctaattttt ccagatcggc
     7861 attggcattc ggcgccgtgg acgtttgcgt ccgttgatgc cagctcagaa tttcgactac
     7921 gcttccgcgt aagagactgc gttctgcttc ttgctgcata cgcaccgcca ttcgagctgt
     7981 accttgtcga ctgtccgtaa aggtaatgag tcgacgtcct cgccctggca acgaatctgg
     8041 tccgacgccc tctttgcctt catcactggt aaagtcctga caatactcaa gaacggtggg
     8101 cacgatatta gtaacgtaaa atggcccacc taatagtgca cgccggaagg gctgtttccc
     8161 actcatgcct ctgtagccac agccactggc gctacaaacc tgttcaacat cattaataat
     8221 cagcggaatg ctgtcattgt taataacgcc aatacggcgc gtctggtggt cgaggcgttg
     8281 tagggtatag cctgtctcgc tggtctctcc tgcggcaata atcaacggag gcaggaaacc
     8341 gttctctttt tcgacttttt cctctgtagt gtcatgttca acaggtactt catcctgcaa
     8401 ggagaactca tcgccacctt tattttccca ttggacaagt ttgccctttt tgtcctgcgc
     8461 cagaagatga ggctcattac aatcattaca gaatgcaact tcgtatacag ggcttccaca
     8521 gtcacaattt tgtcgctggt tcacatacac atagccaaag ggccagcctt tttcgagcgg
     8581 tgtaccgtgc tttaatctgc aatctttatc aacacaagcc catattcctt gggtattacg
     8641 ctggaagata tgcgccctga ctttaagaaa tgcgggatcc tttgcattag gttgagtccc
     8701 tgaacagaca tcgatccaac gcagtacttc ctgctggctg taatgctgtt ttgtcagcgc
     8761 atttaaccgc tgcgtcatct catccagttt cattggattg ggttggctaa ccaacatttc
     8821 acgtatgtaa cgcgcttcgg gggagtgcgt aagcgcataa aagcgctcag ggcttatccc
     8881 tttaatttta ttatcatttt tatcttttag atccagatca ggtatttgct ctatttcgtc
     8941 taacggaaca gaaacgtttt tggatggttc cagttcagga atcacccgac ttccgtccag
     9001 aacatcgatg cgctcctgcg ggataccgga taattcagag agaaattttt tcagctgctt
     9061 ttcggcgtcg ctgcaggcaa tggtcgcaga ggtcgctaca aagcgcacat catctggcgt
     9121 cacgccaaac gctgtcatca cacgacgcag ctggagtgcc agttcagcgg cctgcgaccc
     9181 gacataggta tgcgcttcat ccagtacaat ccagcgtagt gatttttgtg ctttggattg
     9241 ctggataatc ggggcgtcga cctggcgaac catcatatat tccagcatcg tgccgttggt
     9301 caccagaatc ggggctggct ccagacgcat cttttcacgg gataagactt catttggtct
     9361 attatcctgc actgttttca tttttgcgtg gagctcttct gtatttccgt tatacaagca
     9421 gtagcggatc ccagtaccaa aaccacgcgt ccaggcatcc agacgctcgc gctgtgaatt
     9481 aatcaaggcg ttcaatggat agagaaaaag ggctcggaca ccgaccaacg gattgttgcc
     9541 attttcatgc agttcccggt aaagatcttc cagcaccggt accataaaac attccgtttt
     9601 acctgaaccc gtaccactgg ttaccaccac tgaatgtttc ttttccagca aagctttcca
     9661 actggctaac tggtgtgtaa aaggcttcca gtcagcacca aatcggtacc ggccattact
     9721 ctgatcatcc agtgaatcaa ccactgcttt gctcaacaat gcgttgcctt ccgtcagact
     9781 gcgcatcgtg tggtttgact ccttccagcc aaacatttgc tggaataccg gagaagcaag
     9841 gaatgagccc ggtttcccac aatcagcccc catttgctgc gcgagatgtt cacgcaactg
     9901 cgggtttgta acgcccatga tactgagcgt tgcttcagtt gaacgagaca acgattgttc
     9961 aataagtgac gaaaaatagc gtgtagtcat ccttattcct ttttattttt tagcaacata
    10021 acgcaacagg ctgaactgga agatggcctt aaaccagcgg gaatcgaaat ccctgacttg
    10081 cctcaggaaa aatacggcac cgggtttacg gtcaaataca gattcaaaag aagcatttcc
    10141 gcttgccacc gctgcggcga agacgggtaa ccaggccacg gaatagcggt gatttgcgtc
    10201 aggattaata ccaatcactg gatttttttg tgacatcatc catttataca gacgcttgca
    10261 gccgtattca ggccagcgag cttcactgtg ctcacgtaag agctcctgat accacccgtg
    10321 gacacaagat tcaggaatag acggggggag atatccatta cttaaccatt tctcaatttc
    10381 atcggcatat gtcgggaaca cgagccccag ccgttgaaac atattggtaa ccagtaattt
    10441 ctgggtttcc tccggtgcac ctttttggct caggaacaat ttgaaacgct cagatgcagc
    10501 tttgatctca aagatgggga aaaactccca taaaataggg aactcattct caatacgact
    10561 cagatactct gctgacattt cgaatttgaa gagcgacatc gccaatgcct gcggatgctg
    10621 cactagtgca cgccatactt caaaggttgc cagcggtaaa taaccaaatt gatcgtacag
    10681 gctacgcata aattgccagc cggaatgggc aggatcgtta gccatctgtc cgagaacccg
    10741 tgtgatggta ttaacttctg cctgtggatt aaaaagctgg gtcgctttct gcaatgagcg
    10801 gatgttgctt tcttctaccg gtaacgaaga ttcgccacga atgaagcagg ggcgaaaagc
    10861 catctcttca cccggtttag gaacaaccaa ccatggtccg tttttgttaa cgattgagct
    10921 caactcatat tcaccgacag gcactccctc gctcattctt gatgctaagg gaatggattt
    10981 cctctcgggt tcacttaaca gcatgataac gggggaagga atctgtccag cacgatggtt
    11041 cactgactgg ctgagtaata gttctttttc ataatcatag cgcaggctgt atttatagcg
    11101 acgaatttgc caggccatga ccacacccgc ccccgtaata cgcatctcaa caacctgatc
    11161 aatacctgcc tcaagtgaaa tcagattttc aatgtgttca cgtagactat agagattcaa
    11221 ttcaacagga cgctcgctca atgccgtata actccattcg tgccatgcct gtaatccact
    11281 aatggagcgc aaatgcaact gcaatgtata ttttgtcggc tcaccatttc tggagaacag
    11341 gaatgctctg gaacccagca gatcatgcag cgtaatgttt ttatccagag gacgtccgtt
    11401 tacatcaaga gcaaggcagc ctttagctgg gaaaggtaac tcaatgtcga tggtatcagc
    11461 cgccaaatta ggcagaactt gtaatgtaat aaatgccgga ggtacccctt ccgctttgag
    11521 ttgtatctct gttcgcccct cagtacgctt acgtgtagcc tccaatgttt tatctttaag
    11581 tgttgctaca caaggatgct gtgtggtgat tacgatagtg ccttcatttg cgtgcattcc
    11641 accttttatc tcaatactga aatcagcagg caagataccg attttgcgac gcaagagcgt
    11701 ttcattatta ctattacgga tagaaagata ttgagcccct agcatctctt gtatatgaca
    11761 ttgctcaatt gaaattccgc tcaggtaacg cgttacatca atgctttgaa tatctggtag
    11821 tgtagaaatg acttttggta caccaataaa tgtctcatct ggatgacaaa cccagcttgc
    11881 acgctttcca tttagagcaa accttccgat gctgatttgt tctcttccgg tgcgaatccg
    11941 ataagtttca tttgctgtaa ctgtaacgtc ctgacatccc ttaacaggta gcgctttaag
    12001 tcctaatacg tttactgcac tcgattggcc atcaaaacca gcaacagtgc tattgtcccg
    12061 agggagcaca atcagaacat cgctgcttcg cacactgcag gaggcctgtc cctgtaacag
    12121 ccattgatct gcatcaacga tgaaggttaa cggcacctca ccaatggcaa tttcactgtc
    12181 atcaagttta atgctcccaa caatcatccc tccagcacga gccactaacg acaaacctgc
    12241 agatggattt tgcctgacaa atctcacttc gcttttacgt aatcgaactg ttgcctgtct
    12301 gttttccagg gaagcatagg ctggcccaag gccagagact tcctcaccat cttcataaat
    12361 ggcaagttca aagcgcgtag ttgacggctc gctagttaca ggaaaggaaa cttcgtcagg
    12421 aagagatact atcaccctaa gctcatctgg atgcttttct gaccaataga attggcaacg
    12481 cgtactctta tttttttgta gtcgaggctt agcctctaca gaggctgcgc atagcagtcc
    12541 attcaaaaaa tgcgttcctg tttcatcatc taacggtatt ggaaactcgc ttcgccaggt
    12601 agggtgaacc ttatcaagct gttgtactgg ttctttgtga tttgttaggt tgtgagtaag
    12661 tactaaggaa ttgaggttat cagccatatg actgataagt tctacagagg tatcttcgct
    12721 aaaaacagtt ggcagcgctg atttttctat gacagctctt gccagcgaaa gggcagaaaa
    12781 acctgactgc tttgcttgct catattgtcc aaggatcctg gagaaaacag ccagaaagcg
    12841 actgtctgat tctttgagta gccggaaagg taaaccacct tcactgaata atgttccgag
    12901 aaaattacgt cgttcagatt cataaaatcg aattgggcgc agccagtaat cttccatccc
    12961 tttcggtacg atagtaccaa gctctgtggc ggtgaatgaa atccctattt ttttatatat
    13021 agggtcccac gtccatccgc attgccgttc ataatccctg cggtaccact cagagcaaaa
    13081 aagaacaaaa caggcagcaa agtctctatt acatatatta gattgtgatt gaccaacagc
    13141 acagagtagc tgcgttaatt ggttatattc agcattggtt gcatgatatt cataaagagg
    13201 acgtccatcc ggttgtctca accctctaag attcacaaag ttacttagcc aggaagtaca
    13261 ttttacccat gaaatgtatt ctgattgacc caaagcaagc tccttagaga taaaag
//
LOCUS       DAPHJE010000116        13242 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPHJE010000116 DAPHJE010000000
VERSION     DAPHJE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.117"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(615..1706)
                     /gene="ychF"
                     /locus_tag="RH222_003562"
     CDS             complement(615..1706)
                     /gene="ychF"
                     /locus_tag="RH222_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV3798524.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVLPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1823..2407)
                     /gene="pth"
                     /locus_tag="RH222_003563"
     CDS             complement(1823..2407)
                     /gene="pth"
                     /locus_tag="RH222_003563"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV3798525.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEGVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2685..2963
                     /gene="ychH"
                     /locus_tag="RH222_003564"
     CDS             2685..2963
                     /gene="ychH"
                     /locus_tag="RH222_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV3798526.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3018..4697)
                     /gene="dauA"
                     /locus_tag="RH222_003565"
     CDS             complement(3018..4697)
                     /gene="dauA"
                     /locus_tag="RH222_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV3798527.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4822..5769)
                     /gene="prs"
                     /locus_tag="RH222_003566"
     CDS             complement(4822..5769)
                     /gene="prs"
                     /locus_tag="RH222_003566"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV3798528.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5920..6771)
                     /gene="ispE"
                     /locus_tag="RH222_003567"
     CDS             complement(5920..6771)
                     /gene="ispE"
                     /locus_tag="RH222_003567"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV3798529.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6771..7394)
                     /gene="lolB"
                     /locus_tag="RH222_003568"
     CDS             complement(6771..7394)
                     /gene="lolB"
                     /locus_tag="RH222_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV3798530.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLNEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7608..8864
                     /gene="hemA"
                     /locus_tag="RH222_003569"
     CDS             7608..8864
                     /gene="hemA"
                     /locus_tag="RH222_003569"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV3798531.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRGQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8906..9988
                     /gene="prfA"
                     /locus_tag="RH222_003570"
     CDS             8906..9988
                     /gene="prfA"
                     /locus_tag="RH222_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV3798532.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9988..10821
                     /gene="prmC"
                     /locus_tag="RH222_003571"
     CDS             9988..10821
                     /gene="prmC"
                     /locus_tag="RH222_003571"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV3798533.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALGLARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10818..11210
                     /locus_tag="RH222_003572"
     CDS             10818..11210
                     /locus_tag="RH222_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV3798534.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTMLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11214..12023
                     /locus_tag="RH222_003573"
     CDS             11214..12023
                     /locus_tag="RH222_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV3798535.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12059..12913
                     /gene="kdsA"
                     /locus_tag="RH222_003574"
     CDS             12059..12913
                     /gene="kdsA"
                     /locus_tag="RH222_003574"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV3798536.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13061..13168)
                     /locus_tag="RH222_003575"
     CDS             complement(13061..13168)
                     /locus_tag="RH222_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3798537.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3320 a         3329 c         3406 g         3187 t
ORIGIN      
        1 tacttactcc aattcgttac tatgaatatg acaaaaacat tacaccaaac agcggttaaa
       61 taatttggtg aatatttttg gtaataatcc taattagtta tgggatttta gcttgatgta
      121 tctgcataac aaaaacaata tgcgccattt ttgcattact aataagaaaa agcaaaaaac
      181 atcgatattc ttttttgaac aatggatttt ctggcatatc gttattatcg tgctattggt
      241 tatcattttc atcttgccag gatcgcacgt aatcacgtta ttcggggact ttatctgcgg
      301 gatattaccc ttcaggtaag tccttttctc gccagcatat tggagcgtga tcaatattga
      361 tcagctgtga agagggctga tgtgtagtgg aacaaaaact gacgttatat cactgctaat
      421 gagatatccg tcatctgaac acttatgtct ttaattattc tggtggttca ctgtaggcaa
      481 taagcacaaa agtgtaggat gttacaagaa tgattaggac tcggtgaaat gaaaaatcca
      541 cgcaattgcg tggattttat atacttttgc gctcttcatg agatttagcg aaacctcatg
      601 agacaacaaa ttaattagac gttgaaaagg aagttcatca catcgccatc tttaacgatg
      661 taatctttac cttctgcacg cattttgcca gcttctttcg cgccttgttc acctttgtaa
      721 gtgatgaaat cttcaaacga gatggtttgt gcacggataa agcctttttc aaaatcagta
      781 tggattttgc ccgctgcctg cggcgcggtt gctccaaccg gaatggtcca tgcacgcact
      841 tctttcaccc cagcggtgaa gtaagtttgc aggttcagca gcttataacc ggcacggatc
      901 acacggttca ggcccggctc ttccagccca agctcctgca taaactcgtc acgttcttcg
      961 tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa ccggaagcac aacagaacct
     1021 tctttcgccg cgatttcacg cacctggtca agatatgggt tgttttcaaa accgtcttcg
     1081 ttgacgttgg cgatgtacat tgttggtttc agcgtcagga agctcaggta acgaatagcc
     1141 gctttctctt cagcgcttaa atccagcgcg cgcagcatac ctgcgttttc cagctggggc
     1201 aggcattttt ccaggactgc cagctcggct ttcgcgtctt tatcgccacc tttggctttc
     1261 ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc cagttcggtg
     1321 ttaataactt caatatcgtc agccgggtta actttgccgg aaacgtgaat gatgttgtca
     1381 ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt cacggatgtt ggtcaggaac
     1441 tggttaccca gaccttcgcc tttcgatgcg ccttttacca gaccggcgat atcgacaaat
     1501 tccatggtcg tgggaagcgt acgttgcggt tttacgattt cagccagttg atccaggcga
     1561 ggatcaggca ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg aaagttggcc
     1621 gcttcaatac cggctttggt cagcgcgttg aacagggtag atttcccgac gttgggcaaa
     1681 ccgacgatac cgcatttgaa tcccatgatt taaatcacct taatatctta ataatcaacc
     1741 tgttattgct aacagattgc agaaatggaa ataactttgc ctattataca cggcactcgg
     1801 caaaaatgcc gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg ttcgttgctt
     1861 tggtcaagcc atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg atggcttcat
     1921 caattaactt ctgttcacta acaggcggtt tgcctaacac aaaaccgaca actttatttt
     1981 tatcgcccgg atgaccgatt ccgatgcgta aacggtgaaa gttagggtta ttacccaatt
     2041 tactgatgat gtctttcagt ccattgtgac caccatggcc accgcccaac ttaaatttgg
     2101 cgacgccagg aggcagatcc agttcgtcgt gggccaccag aatttcgtcc ggattaatgc
     2161 ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag attcataaat gtagtcggga
     2221 ctaacaggcg gacaccttcg cctccaaggg tgactcgcga agtataacca aagaatttag
     2281 cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac caggcaccag
     2341 cattatgtcg cgttgcggcg tattcagcac cggggttcgc caggccgaca atcaatttaa
     2401 tcgtcacgtt tttttgtcct gagtgtgtac ataactggcg cgtagtttac tggttgcggc
     2461 cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgcc tgagtggact
     2521 attagaaagt cagggtgttc aggcgtttat ttgtaaagtt ttgttgaaat aagggttgta
     2581 attgtgatca cgcccgcaca taacccacag ggtgttgtct atactttaca cataaggaag
     2641 aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa cgcaaaaacg
     2701 cttcgttact cggtaacgtg ctcatggggt tgggtctggt ggtaatggtg gtcggtgtgg
     2761 ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc gcacatggtg
     2821 cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt gttggcgggc
     2881 atgaacaggt gtgcgaccgt tactggtggg ttcgccacta tgacaaacgt tgccgccgta
     2941 gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca gtttgcgctg
     3001 actggtcaat ctcgtactta taaatccgcc atcgccgcgc gacgattcgg gaagaacgcc
     3061 aggcgtcccg ggatcggttg aatgccagcg cgagccatag tgcgcagtgg ctggaattcc
     3121 acgttgcaca cgcgcagttc acatccctcg ggcagacgct tcacaaaacg ctggaacgca
     3181 tcaagaccac cagcatcaag taccggaacg gcatcccact tcagaatcac aatccgtttg
     3241 ccttcaagac gtgactccag gtccgtgaac aagccttcag cagcagcaaa aaacagcggg
     3301 ccaataacac gcagaaccag gacatcgtct ggaacatcta cgactaccgg tgccaggcga
     3361 gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc cacgctgatg
     3421 gcaataacca tatcaaacag cacggtcaac gacatgcaca gcagcatgac aatgatgtca
     3481 tctttcggcg catgacgcag caagtcgacc actttgtgcg cttcactcat gttccacgcc
     3541 accatcaaca gcagggctgc catggcggaa agcggcagcc aggagagcag cggtgccagt
     3601 accagcaggg caagaataac cagaatagag tggatcaccg ccgagatagg ggaagttgcc
     3661 ccggcacgga cgttagcggc agaacgcgcg atggcagctg tagcggtaat accaccaaag
     3721 aacggagcga taatattccc cagtccctgt ccaaccagtt cgctgtttgc cttgtgtttt
     3781 gtcccggtca taccatccag caccacggcg cagagcagag attcgattgc gccgagcatt
     3841 gccattgaga atgccgcagg cagcagtgtg cgaatagaat cccaggtaag cgtgaattct
     3901 gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat accgttaccc
     3961 tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac atgtccgccg
     4021 agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag gtgaccgggt
     4081 aaacgaatgc ccagacgcgg ccagaaaacc agaatgccaa gcgtcacaat gccaatggca
     4141 gcatcaccca cattaatggt cggcagcgcc ataaataatg cgccgacttt ttgtagataa
     4201 tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat ggtaccgatg
     4261 gtgatcccga tacccgaggt gaaacctaag gtgacggaaa ccggaatata ctcaatcagg
     4321 cgaccaaagc gtgccagacc catcagaatc aaaaagatcc ccgacagcaa ggtcgcaacc
     4381 agcagtcctg ccagtccaaa ctgttgcgac acgggataga gaattaccac aaatgccgca
     4441 gtcggaccgg aaacgctaaa gcgtgatcca cccgtcagag caatgacaat ccccgcaaca
     4501 gctgcagtat ataaaccgta ctggggtgcc acaccactac caatagccaa cgccatcgcc
     4561 agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt aaaccgtgcg
     4621 gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat cacatgtgag
     4681 gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc aactaaatgg
     4741 caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac ccgccgcagc
     4801 gggtctttga gccgggttcg attagtgttc gaacatggca gagatcgatt cttcgttgct
     4861 gatacgacga atcgcttcgg ccagcatacc tgacagggtc agagtacgca cgttcggcag
     4921 tgatttgatt tcatcgctca gcggaatggt atcgcagaca acgacttcat caattacaga
     4981 gttacgcagg ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt acgcaaatac
     5041 acgtttagca ccacgttctt tcagagcttc agcagcttta cacagcgtac cgccagtgtc
     5101 gatcatatca tcgaccagta cgcagtcacg acctgcaacg tcaccgatga tatgcatcac
     5161 ctgggaaacg ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg tatcgttcag
     5221 cagcttagcg atagcgcggg cacgcacaac gccgccgatg tccggagaaa ccacaattgg
     5281 gttatccaga ttcagctgca gcatgtcttc cagcaggatc gggctaccaa atacgttatc
     5341 aaccggaacg tcgaagaaac cctgaatctg ttcagcgtgc agatccactg tcagcacacg
     5401 gtcaacaccg acgctggaga ggaagtctgc aaccactttc gcagtgattg gtacacgagc
     5461 ggaacggacg cgacggtcct ggcgcgcata gccaaagtag gggataacag cggtgatacg
     5521 acctgcggaa gcacgacgca gggcatcaac cataacgact aattccatca ggttgtcgtt
     5581 agtaggggca caagtggact ggatgatgaa aatatcacca ccgcgtacat tttcattaat
     5641 ttgtacgctg acttcgccgt cgctaaagcg acctacagcg gcgtcgccga gtgaagtgta
     5701 caggcggttg gcaatacgtt gtgctagttc cggggtggcg ttaccagcaa aaagcttcat
     5761 atcaggcacg agaagaacct caggcatgcg tccattggtg gaaagaatct gccgaaaact
     5821 gtgcgggcca ggcatgatcc tttccaggcg gtgtatttaa gagcgcgatg caacgtctgg
     5881 aacaaggtga cgttgtcacc gaaactcagc ttgcccggct taaagcatgg ctctgtgcaa
     5941 tggggaaaga ttaacgcctt tcgccacaaa gccattgagc cattccgggg cttgctctag
     6001 cacctggcgg gcttcagact ctgtatcaaa ttcagcaaag acacaggccc ctgtcccagt
     6061 caggcgcgac ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct cgcgaaaacg
     6121 ttttcttgcg ataacctcgc aatcattgct gaattcacat tttagcaacg tttctattga
     6181 cctttttggc gtattgcgcg ggagttcagg atctttaaaa atcaccggag tcggaatact
     6241 tacaccaggg tgcgccacca gataccactt ctctggcgga tccaccggcg ttagtatttc
     6301 accaacgcct tcggcaaacg cggcatgccc ccgaacaaag acaggaacat ctgcgcccag
     6361 cgtcagcccc atttccgcca gctcatccat gcttagcccg cattgccaga gatgatttaa
     6421 tgccaccagg accgtcgcgg cattggatga accaccgccg agaccgccgc ccattggcaa
     6481 acgcttgtca atgctgatat ccgcaccgct tcccgtcgga agacgcccgc tgtctgccgc
     6541 agttttcatc aacaaccgcg ctgcgcgaac gatcaggtta tcttcatgtt ccacgccttc
     6601 aacgggcgtt aacagacgaa tatccccatc gtcacgaagc tcaatgctaa tggtgtcgcc
     6661 gtaatcaaga aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac gctgaccggt
     6721 aatgtataaa aacagattaa gttttgccgg agagggccac tgtgtccgca ttatttcact
     6781 atccagttat ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc catattggct
     6841 ggcatcgcag gttgcgtttt ggtgtcataa ccaccgtaaa caactttcca gtttttgcca
     6901 ttctggctgt aggtaatttc gttcaggcgg tactggtcgt ccagtttgta gtcggttgca
     6961 tcacccggta aacctaaaat ccactggcgc aagctgttga gcggaatcgg cattccggtc
     7021 aatttgccaa tcatctcttc ggcgtcatcg gcggtataac gctgaccttt attgtcgact
     7081 aactgcacgt tacccggttg agcattcagc tcaagttccg tactgcccaa tgggttagtc
     7141 agcagcagac ggtagcgatc ctgaccagtt tgctgccaga agaaacgggc gtacactttt
     7201 tgttggtcag aaatataagc gaacgcgccg cgagtttgat actgattaag attgcgcacg
     7261 tcttgctgat gctgacgcca ttgtggcgaa tccgggcttt taccaggacc tttgggcgtg
     7321 gtaacggaac aggcagtgag cacaagagca gccagcggta gcaggcggat aagacgaaaa
     7381 tcgggcaggg gcatagtgat gacaagtcct tgagatacgt tgcagttata acccttaatg
     7441 ctagcgttac cgtccgctat cgtctatgtt caagttgtct taattgccag aatctaacgg
     7501 ctttcggcaa ttactccaaa agggggcgct ctcttttatt gatcttacgc atcctgtatg
     7561 atgcaagcag actaacccta tcaacgttgg tattatttcc cgcagacatg acccttttag
     7621 cactcggtat caaccataaa acggcacctg tatcgctgcg agaacgtgta tcgttttcgc
     7681 cggataagct cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg cagggcggcg
     7741 tggtgctgtc gacgtgcaat cgcacggaac tttatcttag cgttgaagag caggataacc
     7801 tgcaagaggc gttaatccgc tggctttgcg attatcacaa tcttaatgaa gaagatctgc
     7861 gtaaaagcct ctactggcat caggataacg acgcggttag ccatttaatg cgtgttgcca
     7921 gcggcctgga ttcactggtt ctgggggagc cgcagatcct tggtcaggtt aaaaaagcgt
     7981 ttgccgattc gcaaaaaggc catatgaagg ccagcgaact ggaacgcatg ttccagaaat
     8041 ctttctctgt agcgaaacgc gttcgcactg aaacagatat cggtgccagc gctgtgtctg
     8101 tcgcttttgc ggcttgtacg ctggcgcggc agatctttga atcgctctct acggtcacag
     8161 tgttgctggt aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg cgcgaacata
     8221 aagtacagaa gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt ctggcagatg
     8281 aagtcggcgc ggaagtgatt gccctgagcg atatcgacga acgtctgcgc gaagccgata
     8341 tcatcatcag ttccaccgcc agcccgttac cgattatcgg gaaaggcatg gtggagcgcg
     8401 cattaaaaag ccgtcgcaac caaccaatgc tgttggtgga tattgccgtt ccgcgcgatg
     8461 ttgagccgga agttggtaaa ctggcgaatg cttatcttta tagcgttgac gatctgcaaa
     8521 gcatcatttc gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg gaaactattg
     8581 tcgctcagga aaccagcgaa tttatggcgt ggctgcgagc acaaagcgcc agcgaaacca
     8641 ttcgcgagta tcgcggccag gcagagcaag ttcgcgatga gttaaccgcc aaagcgttag
     8701 cggcccttga gcagggcggc gacgcgcaag ccattatgca ggatctggca tggaaactga
     8761 ctaaccgctt gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt gacggggata
     8821 acgaacgcct gaatattctg cgcgacagcc tcgggctgga gtagcagtac atcattttct
     8881 ttttttacag ggtgcattta cgcctatgaa gccttctatc gttgccaaac tggaagccct
     8941 gcatgaacgc catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta tcgccgacca
     9001 ggaacgtttt cgcgcattat cacgcgaata tgcgcagtta agtgatgttt cgcgctgttt
     9061 taccgactgg caacaggttc aggaagatat cgaaaccgca cagatgatgc tcgacgatcc
     9121 tgaaatgcgt gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa gcgagcaact
     9181 ggaacagcaa ttacaggttc tgttactacc aaaagatcct gatgacgaac gtaacgcctt
     9241 cctcgaagtc cgtgccggaa ccggtggcga cgaagcggcg ctgttcgcag gcgatctgtt
     9301 ccgtatgtac agccgttatg ccgaagcccg ccgctggcgg gtagaaatca tgagcgccag
     9361 cgagggtgaa catggtggtt ataaagagat catcgccaaa attagcggtg atggtgtgta
     9421 tggtcgtctg aaatttgaat ccggcggtca tcgcgtgcaa cgtgttcctg ctacggaatc
     9481 gcagggtcgt attcatactt ctgcttgtac cgttgcggta atgccagaac tgcctgacgc
     9541 agaactgccg gacatcaacc cagcagattt acgcattgat actttccgct cgtcaggggc
     9601 gggtggtcag cacgttaaca ccaccgattc ggcaattcgt attactcact tgccgaccgg
     9661 gattgttgtt gaatgtcagg acgaacgttc acaacataaa aacaaagcta aagcactttc
     9721 tgttctcggt gctcgcatcc acgctgctga aatggcaaaa cgccaacagg ccgaagcgtc
     9781 tacccgtcgt aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta cttacaactt
     9841 cccgcagggg cgcgttaccg atcaccgcat caacctgacg ctctaccgcc tggatgaagt
     9901 gatggaaggt aagctggata tgctgattga accgattatc caggaacatc aggccgacca
     9961 actggcggcg ttgtccgagc aggaataatg gaatatcaac actggttacg tgaagcaata
    10021 agccaacttc aggcgagcga aagcccgcgg cgtgatgctg aaatcctgct ggaacatgtt
    10081 accggcaaag ggcgtacttt tatcctcgcc tttggtgaaa cgcagctgac tgacgaacaa
    10141 tgtcagcaac ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat tgctcattta
    10201 accggggtgc gagaattctg gtcgttgccg ttatttgttt cgccagcgac cttaattccg
    10261 cgcccggata cggagtgtct ggtggagcag gcactggcgc ggttgcctga acaaccttgc
    10321 cgtattctcg atctcgggac gggtaccggg gcgattgcgc ttgggctggc tcgcgagcgc
    10381 ccggactgcg aaattaccgc tgtagatcgt atgcccgatg ctgtcgccct ggcacaacgt
    10441 aatgcccagc atctggcgat caaaaatatc cacattctgc aaagcgactg gtttagcgcg
    10501 ctagccgggc agcagtttgc gatgattgtc agcaatccgc cgtatattga cgagcaggac
    10561 cctcatcttc aacaaggcga tgtccgcttt gagccgctca ctgcgctggt tgcggcagac
    10621 agtggaatgg cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct ggtatccggc
    10681 ggctttctgc ttctggaaca tggctggcag cagggcgaag cggtgcggca ggcatttatc
    10741 ctcgcggggt atcatgacgt cgaaacctgc cgtgactatg gtgataacga gcgcgtaacg
    10801 ctcggccgct attatcaatg acaagttttt ctacactgct tagtgttcat cttattagta
    10861 tcgcgctttc tgttgggcta ttaaccttac gtttctggct acgttatcag aagcatccac
    10921 aggcatttgc tcgctggacg cgcattgtgc cgccggttgt cgatacgatg ttattgttaa
    10981 gcggcattgc gttgatggct aaagcgcaca tcctgccatt ttctgggcag gcacagtggc
    11041 tgactgaaaa gctgtttgga gttatcattt atatcgtttt gggttttatt gcactcgatt
    11101 atcgtcgtat gcacagtcag caggcgcgca ttattgcctt cccgctggcg ttggtggtgc
    11161 tgtacatcat cattaaactc gccaccacaa aagtaccgtt actggggtaa gtcatgagat
    11221 cgttagctga tttcgaattt aataaagcgc cattgtgcga aggcatgatc ctggcttgcg
    11281 aagcaatccg ccgcgatttt ccctcgcaag atgtttacga cgaactggag cgtctcgtta
    11341 gtctggcgaa ggaagaaatc agccagcttc tgcctttaga agagcagctg gaaaaactga
    11401 tcgcgctgtt ttacggcgac tggggattta aagcctcacg cggcgtttat cgtctttccg
    11461 atgcattatg gctggaccag gtgttaaaga atcgacaggg cagtgcggta tcattaggtg
    11521 cggttttatt atgggtcgcg aatcgtctcg atttgccgct gctgccggtg attttcccta
    11581 cgcagctgat attgcgcatt gaatgtccgg atggcgaaat ttggctgatt aatcctttta
    11641 acggtgaatc gttaagcgaa catatgctgg acgtatggtt aaagggaaat atcagcccgt
    11701 cggcggaact gttttatgaa gaccttgatg aagctgataa cattgaggta atccgcaaat
    11761 tgctggatac actcaaagcc tcgttgatgg aagaaaatca gatggagctg gcgttacgca
    11821 ccagcgaagc tttattacaa ttcaaccctg aagatcccta tgaaattcgc gatcgcgggt
    11881 tgatttatgc gcaactggat tgcgaacacg ttgcgttgaa cgatttaagt tatttcgttg
    11941 aacagtgtcc ggaagacccg atcagcgaaa tgatccgtgc gcaaataaat aacatcgcgc
    12001 ataaacatat tgtgctgcat taattaatcg acattttact caagattaag gcgatcctat
    12061 gaaacaaaaa gtggttagca ttggcgacat caacgtagca aatgacctgc cgttcgtact
    12121 gtttggtggt atgaacgtgc tggaatctcg cgatctggcg atgcgcattt gcgagcacta
    12181 cgtaaccgtg acccagaaac tgggtatccc ttacgtgttc aaagcctctt ttgacaaagc
    12241 caaccgctcc tccatccact cttatcgtgg accgggcctg gaagaaggga tgaaaatctt
    12301 ccaggaattg aagcagactt ttggcgtgaa aattatcacc gacgttcacg aaccaagtca
    12361 ggcacagccc gttgctgatg tcgtggatgt gattcagttg ccggcgtttc ttgctcgcca
    12421 gactgacctg gttgaagcca tggcgaaaac cggtgcggta attaacgtca agaaaccaca
    12481 gtttgtcagc ccgggacaga tgggtaatat cgttgataaa ttcaaagaag gcggcaacga
    12541 aaaagtgatt ctttgcgatc gcggtgctaa cttcggctat gacaacctgg ttgtcgatat
    12601 gctgggcttc agcattatga agaaagtgtc tggtaactcg ccggtgattt tcgacgtgac
    12661 ccacgcactg caatgccgcg atccttttgg cgcagcttcc ggcggtcgtc gtgctcaggt
    12721 ggctgagctg gcacgagccg gtatggcggt aggtctggcg gggctgttta ttgaagcgca
    12781 tccggatccg gaacatgcga aatgtgatgg tccatccgcg ctgccgctgg ctaaactgga
    12841 accgttcctc aagcagatga aagcgattga tgatctggtg aaaggtttcg aagaactgga
    12901 taccagcaag taatcttttt gtttgaaaaa taaagtctta gcgttctgcg ttaagacttt
    12961 ttttatgggt gccggataca aaaaaggccg caggctgtta cccctgcggc cggtttcggg
    13021 cgcatattgc catcacggca gcctgacgcc cgttttcgcc ttacttccgg ttacgccacc
    13081 agccgacaat cgctgcggta ataattcccg ccaggatcgg tgctgccagg tcgtgccaga
    13141 aaatcatggc aaactgcgcg agcgtcatat agccgccttg ttgtaatgac aacattttgc
    13201 ggctattctt gaattgttga tggttcaaga ttagcccccg tt
//
LOCUS       DAPHJE010000117        13148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPHJE010000117 DAPHJE010000000
VERSION     DAPHJE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.118"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..438)
                     /locus_tag="RH222_003576"
     CDS             complement(<1..438)
                     /locus_tag="RH222_003576"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HDV3798538.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLAN"
     gene            complement(460..1509)
                     /gene="allD"
                     /locus_tag="RH222_003577"
     CDS             complement(460..1509)
                     /gene="allD"
                     /locus_tag="RH222_003577"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV3798539.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGNEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            1826..3493
                     /locus_tag="RH222_003578"
     CDS             1826..3493
                     /locus_tag="RH222_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV3798540.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            3503..4762
                     /locus_tag="RH222_003579"
     CDS             3503..4762
                     /locus_tag="RH222_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV3798541.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWVKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            4773..5588
                     /locus_tag="RH222_003580"
     CDS             4773..5588
                     /locus_tag="RH222_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV3798542.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            5585..6478
                     /locus_tag="RH222_003581"
     CDS             5585..6478
                     /locus_tag="RH222_003581"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV3798543.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQKLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(6617..7684)
                     /gene="purK"
                     /locus_tag="RH222_003582"
     CDS             complement(6617..7684)
                     /gene="purK"
                     /locus_tag="RH222_003582"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV3798544.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRIGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(7681..8190)
                     /gene="purE"
                     /locus_tag="RH222_003583"
     CDS             complement(7681..8190)
                     /gene="purE"
                     /locus_tag="RH222_003583"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV3798545.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(8308..9030)
                     /gene="lpxH"
                     /locus_tag="RH222_003584"
     CDS             complement(8308..9030)
                     /gene="lpxH"
                     /locus_tag="RH222_003584"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV3798546.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQGLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(9033..9527)
                     /gene="ppiB"
                     /locus_tag="RH222_003585"
     CDS             complement(9033..9527)
                     /gene="ppiB"
                     /locus_tag="RH222_003585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV3798547.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            9701..11086
                     /gene="cysS"
                     /locus_tag="RH222_003586"
     CDS             9701..11086
                     /gene="cysS"
                     /locus_tag="RH222_003586"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV3798548.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(11122..11643)
                     /locus_tag="RH222_003587"
     CDS             complement(11122..11643)
                     /locus_tag="RH222_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3798549.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(11751..11963)
                     /gene="ybcJ"
                     /locus_tag="RH222_003588"
     CDS             complement(11751..11963)
                     /gene="ybcJ"
                     /locus_tag="RH222_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV3798550.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(11965..12831)
                     /gene="folD"
                     /locus_tag="RH222_003589"
     CDS             complement(11965..12831)
                     /gene="folD"
                     /locus_tag="RH222_003589"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV3798551.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         3029 a         3459 c         3668 g         2992 t
ORIGIN      
        1 attcgccagc ccaaagatat ttttactgcc ccagaagaca tacgggaagc ggctgccttc
       61 ttcttctgcc atcgccacca cttcgaccgt acgtagcggc gcgccgtatt gcgttttcag
      121 ccagtcaatt gccagccacg ccgccagcgc gccgaattgc ccgtcgaggt taccgccgtt
      181 aaccacggta tcgatatgcg aaccgctcag aaccacttct tgtggatatt cggtgccact
      241 caggcgacca tataaattcc ccacttcatc gaaacgtgtt tccagcccgc ttgctgccat
      301 tcttttttta aattgctgct gagtttccag ccattccggc gaataaagta aacgggtcat
      361 accacccgtt gggtcagcgc caaaagagga aagccagggc agcgtttctt ctatggcttg
      421 acgaaaatgt gtaatcataa gaaagtcctg tctcaataat tattgcgcaa agggattttt
      481 cgtttcgtat gacgtgttat aaagcgcgtc ggaaattaaa tactggtaaa tatcatcaac
      541 aatttcgatg ccttcgacgg cggctttgcg ttgtttaata tcctgatcct gtccgggata
      601 ataaacctga ttaaaaccgg gcgcgggggt aatggcattt aattcgcgca tggtctggct
      661 aagatgttga cggaataatt cgctggagga gaaaaagttc gggttaataa ctacatgtaa
      721 ttgccccaaa ttacgcccgg cgtgtaaatc gtcatacatc gaactaacct gtcgcccgaa
      781 cggtaagccg agtaagacgc ctgagaggac gtcaatcatc atcatcaggc catacccttt
      841 cggcccggcg gcggggagca gagcatgtac cgcgaacgga tcggttgttg gtgcaccgtt
      901 tttatcgacc gcccaagtat ccgggataga catattacgc gagcgggcgt cgagcacttt
      961 tccccatgcc tgtacggtag tcgccatatc aaaggtaagg atctcgttgc cttctcccgg
     1021 cgcggcaaag gccagtgggt tagtaccgta gtaaatttcc gcgccgccaa acggcaccac
     1081 cattggatcg gactggcaca tcgaaatgcc aattaatccg gcgcgggctg cctgctgcac
     1141 aaaataagag attgcgccgc tgtgacccat ccggctgata ccgaccaccg caacgccatt
     1201 ttgctgggcg gttttgatgg catgttccat acccattttc gccgcgacct gtccggcggc
     1261 attgtcggca tgtaaaattg ccgagcacgg cccggtttcc tcaagacgaa actccggttc
     1321 gcggttggta ccgccttttg aaatgcgttc cgcgtagtat tccacgcgca ccgcgccatg
     1381 agagtggatc cctctggcat cggcgtaaac caatacttca gccacggttg cagcgtgctc
     1441 acgttttaac ccagcctggc agagtttatt ctcaattagc tggtggagtg tttcccgact
     1501 gattttcatc tgtcttcctt tttaacgacg gtgtgaagca tgactgcaat taacatacag
     1561 ggaaaatatc tggattatgt gatccagaca ggcaaaaaaa tatagttaga ttttatttga
     1621 taatccgctc gcttttaacc tggtttttaa aacaaccacg cttattaaaa aataacacgt
     1681 aatagcatgg cggatatttg aattcttttg ttaataatcc ctgtgtgata ttcatcacct
     1741 tatttacccg ttgtcatcga taccgtaatc gccacattaa cactgctcgt gcaattgcca
     1801 tgggtgcaat ttttaaggag ttgttatgat ccacgccttt attaaaaaag ggtgttttca
     1861 ggattcggtc agtttaatga ttatttcacg aaaactcagc gaatcagaaa atgttgatga
     1921 tgtttccgta atgatgggta cgcccgccaa taaagcgtta ttagatacca caggtttctg
     1981 gcatgacgat tttaataacg ccacgccgaa cgatatttgc gtggcaattc gtagcgaagc
     2041 ggcggatgcg gggatcgcgc aggcgattat gcagcagctt gaagaggcgc taaaacaact
     2101 ggcgcagggg gcaggcagca gccaggcgtt gacgcaggtg cgtcgctggg acagtgcctg
     2161 tcagaaatta cccgacgcca gtctggcgct gatttcagtg gctggtgagt atgcggcgga
     2221 gctggcgaac caggcgctgg atcgcaacct caacgtgatg atgttctccg ataacgtcac
     2281 gctggaagat gaaatccaac ttaaaacccg cgcgcgggaa aaaggcttgc tggtgatggg
     2341 gccggactgc ggtacatcga tgattgccgg cacgccgctg gcttttgcta acgtgatgcc
     2401 ggaaggcaac attggcgtca ttggcgcttc cggtaccggg attcaggagc tgtgctcgca
     2461 gattgcgctg gcaggggagg gaattactca cgcgattggc cttggcgggc gcgacctcag
     2521 ccgtgaagta ggcggcatca gtgcgctgac ggcgctggaa atgctcagtg cagacgagaa
     2581 aagcgaagtg ctggcatttg tttcaaaacc gcctgccgaa gctgtgcgtc tgaaaattgt
     2641 taatgccatg aaagcaaccg gcaaaccgac ggtggcgctg tttttaggtt ataccccggc
     2701 ggtggcccgc gacgagaatg tctggtttgc ctcctcgctg gatgaggccg cacgcctggc
     2761 ttgcctgctt tcacgtgtca cggcgcgacg taacgcaata gcgcctgtca gcagcggatt
     2821 tatttgcggt ttgtataccg gcggtacgct ggctgccgaa gcggcgggat tacttgctgg
     2881 acaccttggc gtggaagccg acgataccca tcatcacggc atgatgctcg acgccgatgg
     2941 tcaccagatt attgacctcg gcgatgattt ctacaccgtc gggcgtcccc atccgatgat
     3001 cgacccagcc ttacgcaacc agttaattgc cgatctcggc gctaaaccgc aagtgcgcgt
     3061 attgctgctt gatgtcgtga ttggcttcgg tgcgaccgcc gatcctgccg cctcgctggt
     3121 aagcgcctgg caaaaagcat gtgccgcgcg ttcagacaat caaccactgt atgccattgc
     3181 cacggtgaca ggcactgaac gtgacccgca atgccgctcg cagcaaatcg ccacgctgga
     3241 agatgcaggg atcgcggtag tgagttcgct gccggaagcc accttgctgg cggcggcact
     3301 gattcgtccg ctttcgcctg ccactcagca acacacaccg tcattactgg aaaacgtcgc
     3361 cgtgattaac atcggattac gcagctttgc gctggagcta caaagcgcca gcaaaccggt
     3421 tgtgcattac caatggtcgc cagtcgccgg cggcaataaa aaactggctc gtttattaga
     3481 acgtttgcaa taaggggttc ccatgtttac atcagtagcg caagccaatg ctgcggttat
     3541 cgaacaaatt cgtcgcgctc gtccacactg gctggatgtg caaccggctt cttcacttat
     3601 cagcgaacta aacgagggca aaacactgct tcacgccggg ccgccaatgc gctggcagga
     3661 gatgaccgga cccatgaaag gggcgtgcgt gggcgcatgt ctgttcgaag gctgggtgaa
     3721 agatgaagcg caggcgctgg caatactgga gcagggggag gtgaacttca ttccttgtca
     3781 ccatgtgaat gccgtcggtc caatgggcgg tattacttct gccagtatgc cgatgctggt
     3841 ggtcgagaac gtgaccgacg gcaaccgggc gtactgcaac ctcaacgaag gtatcggcaa
     3901 agtgatgcgt tttggcgctt acggcgaaga tgtcctgact cgccatcgct ggatgcgcga
     3961 tgtgttaatg ccagtattaa gcgcggcgct ggggcgcatg gagcgcggta tcgatctcac
     4021 ggcgatgatg gcgcagggca ttacgatggg cgatgagttc catcaacgca atattgcttc
     4081 ctctgcactg ttaatgcgtg cgctggcccc acaaattgct cgcctcgatc atgataaaca
     4141 gcacatcgcc gaagtgatgg atttcctcag cgtgaccgat cagttcttcc tcaacctcgc
     4201 gatggcttac tgcaaggcgg cgatggatgc tggcgcgatg atccgcgcag gcagcatcgt
     4261 cacggcaatg acccgcaacg gcaatatgtt cgggattcgg gtaagcgggc tgggcgaacg
     4321 ctggtttact gcgcctgtaa acactccgca aggtctgttt ttcaccggct tctcgcagga
     4381 gcaggcgaac ccggatatgg gcgatagcgc gattaccgaa acctttggta tcggaggtgc
     4441 ggcaatgatc gcagcgcctg gcgtaacgcg ctttgtcggt gcgggtggca tggaagcggc
     4501 cagagcggta tctgaagaga tggcggaaat ttaccttgag cgcaatatgc agttgcagat
     4561 cccaggctgg gattttcagg gcgcgtgcct gggactggac attcgtcgcg tggtagaaac
     4621 cggcattacg ccactcatca ataccggtat cgcccataaa gaggcgggga tcgggcagat
     4681 tggcgcaggc actgtgcggg caccgctggc gtgttttgaa caggcgctgg aagcactggc
     4741 tgaaagcatg gggattggtt gaggaacgcg caatgacgat catccatcct ctgcttgcca
     4801 gtagtagcgc accgaattat cgccagtcct ggcggttagc gggagtgtgg cggcgggcga
     4861 ttaacctgat gacggaaagc ggcgaactgt taacgctgca tcgtcagggt agtggtttcg
     4921 gccccggagg atggatgctt cgccgtgcgc aattcgatgc gttatgcggt ggattatgcg
     4981 gcaatgaacg accacaggtt gtggctcaag ggattcgcct cgggcgtttc acggttaaac
     5041 agccacagcg ttattgtttg ctgcgtatta cgccgcctgc gcatcctcaa ccacttgcag
     5101 ctgcatggat gcaacgcgcg gaggaaaccg ggcttttcgg gccactggcg ttggcggcaa
     5161 gcgatccgct gcctgctgag ttacgccagt ttcgtcactg ttttcaggcc gcgctcaatg
     5221 gcgttaagac cgactggcgg cactggctgg gtaaaggccc cggattaacg ccgagtcatg
     5281 atgacacgct gagcggaatg ctgctggcgg cctggtatta tggcgcttta gatgcgcgct
     5341 ccggtcgcca gttttttgcc tgttccgaca atctgcaact cgttaccaca gcggtgagcg
     5401 tcagttattt acgttatgcc gcgcaaggat atttcgcctc gccactcctg cactttgttc
     5461 atgctctgag ttgcccgaaa cgtaccgctg ttgcgattga ttcgctgctg gcgctggggc
     5521 atacgtcagg ggcagataca ctgctggggt tctggcttgg tcaacaatta ttacaaggaa
     5581 aaccatgaaa acactggttg tagctcttgg gggcaacgcc ttactccagc gcggtgaggc
     5641 gctgacggca gaaaatcaat atcgcaatat cgccagtgct gtacccgcgc tggcgcgcct
     5701 ggcccgttct tatcggctgg cgattgttca cggcaacggg ccgcaggtgg ggctgctggc
     5761 attgcagaag ctggcgtgga aagaggtaga accgtatccg ctggatgtgc tggtggcaga
     5821 aagtcagggg atgattggct atatgctggc gcagagtttg agcgcacagc cgcagatgcc
     5881 gcccgtgacg acggtgctga cgcgcattga ggtttcgcct gatgatccgg cgtttttgca
     5941 gccagagaaa tttattggtc cggtttatca gccagaagaa caagaggcac tggaagcggc
     6001 ttacggctgg cagatgaaac gtgatggtaa atatttgcgc cgggtggtgg cgtctccgca
     6061 accgcgtaaa attctcgaca gcgaagccat cgagttgttg ctcaaagagg ggcatgtggt
     6121 gatttgcagt ggcggcggcg gtgtgcctgt gacggaagat ggggcaggga gtgaagcggt
     6181 gattgataaa gatctcgctg ctgcgttgct cgctgagcag attaacgccg atgggctggt
     6241 gatcctcact gatgctgatg cggtatatga aaactgggga acgccgcagc aacgcgccat
     6301 tcgccatgcc acaccggatg agttagcgcc atttgccaaa gccgatggtt cgatggggcc
     6361 gaaggtaacg gcggtgagtg gttatgtcag aagccgtggt aaacccgcgt ggattggggc
     6421 gttatcgcga attgaagaga cgctggcggg cgaagcgggg acctgtattt cgctgtagtg
     6481 atgagagcat ccagcaatcg gtgcaccatg cctgatgcga cgcttgacgc gtcttatcag
     6541 gcctacaatg ggtaccggat cggtgtaggc cggataaggc gttcacgccg catccggcaa
     6601 tcaaatgagc accaggttaa ctgaacttac tctgtgccca catcacgccg ctggcatatt
     6661 ccggcggcag cagcgggatt aaggcttcca gcgtcgcgga cagacgcgat gtgtcgctgt
     6721 cggtcaaatt cagatgcccc actttacgcc ccggacggac ttctttgtcg taccagtgca
     6781 gatgcaccag cggcaatttc agccagtcat aattcacatc gctaccaatc agattgatca
     6841 tcaccgacgg actattcact actggttgcg gtaacggcag atcggtaatc gcccgcagat
     6901 gcagctcaaa ctggctgatg ctggcaccgt tttgtgtcca gtgaccgctg ttatgcacac
     6961 gcggagccag ttcgttgatc agcagacctt gcggggtgac aaaacactcc atcgccatca
     7021 cgcccacata gcccagctcc tgcataatcg ccgacagcat ctcttcggct tgcgcctgct
     7081 gctgtgcgtt ggcctgcgga aaagcgacgc tggtgcgcaa aataccgtcc tgatgcaggt
     7141 tatgcgtcag cggataaaac acggtgctgc catcaaagcc gcgcgcgcca accagcgaca
     7201 cttcaccaga gaagttaatg ccctgctcga caatacattc gccgtaacac tctgccggta
     7261 actgttcggt ttcatttgcg cgtaaacgcc attgaccgcg accgtcataa ccaccaatgc
     7321 gacgcttaac aatcgccagc tcacctaaac gatcaaacac cgcaggccac tcgctgcgtt
     7381 cggcaagtaa ctgccacggt gcagtcggca ggtggagctt atcgaaaagc tgcttctgag
     7441 tcagacggtc agcaataatc gggaacacat cgcggttcac aaaggccgga tggcgcgcca
     7501 gctcgcgggt taatgcggtt tccggccagc gttctatctc agcggtaatc acgctttgtt
     7561 gaaaaggcac cgccgccggt tcagcgtcca gcccgactgg ccagacagca atgcctaacg
     7621 gttcgcctgc ctgacgcagc atacggccta actgcccgtt accgaggacg caaacctgtt
     7681 tcatgccgca cctcgcgggt ccgggttttc cagcacttcg tcggtctggg ctttgcgcca
     7741 gtcattcaga cgctggtgca gttctttatc atgagtcgca agaatttgtg ctgccagtaa
     7801 cgccgcgttt gccgcgccag ctttaccaat cgccagcgta cccaccggaa tgccgcgcgg
     7861 catttgtacg atggagtaga ggctatcgac accgctcagt gcggcgctct gtactggcac
     7921 gcccagcacc ggcaccagcg ttttggcggc aatcatgcct ggcagatgcg ctgcgccgcc
     7981 tgcgcccgca ataatcacct gataaccgtt ctcttcggcg ctttcggcga agctgaacag
     8041 tttatcgggg gtgcggtgag cagaaaccac ttcaacgtgg tgcgggacat tcaggatttc
     8101 gaagatttcg gcggcgaact gcatggtagc ccagtcgctt ttggacccca tcacgatggc
     8161 gacacgcgcc ggattattgc gggaagacat gcgtcttaaa actcctgtgg tgcacaactc
     8221 tcggctttag agggcacaga gaatagcacg gaaagagagc aaggaaaacg gttgcgtggc
     8281 tgtgaaatca gcaaagttgc gggtttttta aaacggaaaa tgaattagct caacgtcatc
     8341 cgccgtgact ttcaccatcg aaccttccgt atgccaggca cccagtacca cgcgaaaagc
     8401 aggttgctga ttggcgataa gttcatgcac cgccgggcga tgggtatgcc cgtggatcag
     8461 cccttgcacc tgatgttttt ccatcgcact gactaccgcg ttttggttaa cgtccatgat
     8521 cgccagcgat ttactgctat tggcttcttt actgttcgcg cgcattcgcg cggcaatgcg
     8581 tttgcgcaca aacaacggca gagcgaggaa tagcatttgc agccagggtt tgtggacttt
     8641 ggcgcgaaaa gcctgataac ccgcgtcatc ggtgcacagc gtgtcgccat gcataatcaa
     8701 cacccggcga ccataaagtt cgagcacctt ttcttccggc aataatgtca tgccgctttc
     8761 acgggcaaag cgtttgccga gcagaaaatc acggttgcca tgaatgaaat aacagggaac
     8821 gccggaatcg gacaccgctt tgatcgctgc cgccatctgg cgatggagtg ggttgggatc
     8881 gtcgtcgcca atccatgctt caaacagatc gccaagaata tacagcgcgt cggccttgcg
     8941 ggcttccccc gctaaaaaac gcagaaaacc ggcggtgatc gccggttctt ccacgcagag
     9001 atgaagatct gcaataaaga gtgtcgccac gattactcgc taacggtcac gctttcaatg
     9061 ataacgtctt cttttggcac gtcctggtgc ataccgctac gaccggttgc aacacctttg
     9121 attttgtcta ccacgtccat gccgtcaacc acttcagcaa acacgcagta gccccaacct
     9181 tgcaggcttt cgccagagaa gttcaggaag tcgttatcaa ccacgttgat gaagaactgt
     9241 gcagttgcag agtgcggagc ctgagtacgt gccattgcca gcgtaccacg ggtatttttc
     9301 aggccgttgt tggcttcgtt tttgatcggt tctttggtgg ctttttgttt catgcccggt
     9361 tcaaaaccgc cgccctgaat cataaagccg ttgataacac ggtggaaaat ggtgttgttg
     9421 taaaaacctt cgcggcagta gtccaggaag tttttaactg tttcaggtgc tttatcgtca
     9481 aaagttttga tgacaatatc gccgtgattg gtgtggaaag taaccatttt tgcatcctgt
     9541 tccgtttgat tggtgcttca acccagttcg ggtcatatat agggtggtgt tatagcataa
     9601 ccgcacgatc ggatcatcac gcaatgtatg ctgattcgcg cgggaaatat gggtattata
     9661 cgcaactcaa ttacccacac atgtctaaac ggaatcttcg atgctaaaaa tcttcaatac
     9721 tctgacacgc caaaaagagg aatttaagcc tattcacgcc ggggaagtcg gcatgtacgt
     9781 gtgtggaatc accgtttacg atctctgtca tatcggtcac gggcgtacct ttgttgcttt
     9841 tgacgtggtt gcgcgctatc tgcgtttcct cggctataag ctgaagtatg tgcgcaacat
     9901 taccgatatc gacgacaaaa tcatcaaacg cgccaatgaa aatggcgaaa gctttgtggc
     9961 gctggtggat cgcatgatcg ccgaaatgca caaagatttt gatgcgttga acattctgcg
    10021 cccggatatg gagccgcgcg cgacgcacca tatcgcagaa attattgaac tcactgaaca
    10081 actgatcgcc aaaggtcacg cttatgtggc ggacaacggc gacgtgatgt tcgacgtccc
    10141 gaccgatcca acttatggcg tgctgtcgcg tcaggatctc gaccagctgc aggcaggcgc
    10201 gcgcgttgac gtggtcgacg acaaacgcaa cccaatggac ttcgttctgt ggaagatgtc
    10261 gaaagagggc gaaccgagct ggccgtctcc gtggggcgcg ggtcgtcctg gctggcacat
    10321 tgaatgttcg gcaatgaact gcaagcagct gggtaaccac tttgatatcc acggcggcgg
    10381 ttcagacctg atgttcccgc accacgaaaa cgaaatcgcg cagtccacct gtgcccatga
    10441 tggtcagtat gtgaactact ggatgcactc ggggatggtg atggttgacc gcgagaagat
    10501 gtccaaatcg ctgggtaact tctttaccgt gcgcgatgtg ctgaaatact acgacgcgga
    10561 aaccgtgcgt tacttcctga tgtcgggcca ctatcgcagc cagctgaact atagcgaaga
    10621 gaacctgaag caggcgcgtg cggcgctgga gcgtctctac actgcgctgc gcggcacaga
    10681 taaaaccgtt gcgcctgccg gtggcgaagc gtttgaagcg cgctttattg aagcgatgga
    10741 cgacgatttc aacaccccgg aagcctattc cgtgctgttt gatatggcgc gtgaagtaaa
    10801 ccgtctgaaa gcagaagata tggcagcggc gaatgcaatg gcatctcacc tgcgtaaact
    10861 ttccgccgta ttgggcctgc tggagcaaga accggaagcg ttcctgcaaa gcggcgcgca
    10921 ggcagacgac agcgaagtgg ctgagattga agcgttaatt caacagcgtc tggatgcccg
    10981 taaagcgaaa gactgggcgg cggcagatgc ggcgcgtgac cgtcttaatg agatggggat
    11041 cgtgctggaa gatggcccgc aagggaccac ctggcgtcgt aagtaattgc gctattgccg
    11101 gatgcgagtt ttcgcatccg gttatcgtct gcgccaccac aacattccca tcagtagcat
    11161 ccccggcaac cacacccaca tcaattcaga aataatcacc tgatgcccgt acgctgtggt
    11221 gtaacgagac aatgcaaacg gcgcgacttt aatcacctgc cagggagcga aaaagcgttc
    11281 atctgaccac ggccacagcc agccaacccc tttaccgcca gtggttaccg aatccagcaa
    11341 gctgtgcgat agcaacgaga cggttaaaaa cagccagcag cgaatcagcc cagccctgaa
    11401 ccatcgacgt ccaatcagca cacataacag cgggacaaca aacgcaaaca ccagcgaatg
    11461 ggtaaacccg cgatgaccaa aaacattgcc gtaagcaacg ccaaaattaa acgacaagac
    11521 gtcggcatcg ggcagcatcg ccaggatgat tccggcaaat aacaggcgcg gagggatgac
    11581 tttcgaaccc aaccctaaac caatgcatag gggaacggcg gcgtgcgtaa taacggttgg
    11641 catgatggtc gcttcggcaa aatgtcgatg ctatcagcat ggatgaacgg ggcgtagagg
    11701 gcaaaagtct gaaaagagaa ccggcctgtt gatacaggcc gggaaaggga tcaggcaaca
    11761 acctgtacgc tgtgacctgc aaaactcact gtctgaccgg cgacgatttt gcagcgtttg
    11821 cgcgtttcaa ccgcaccgtc gactttcacc tggccttcgg caatcgcgat tttcgcctgc
    11881 gcgccgcttt cgctccagcc ttccagtttc agcaagtcgc acagctcaac gtgcggatgt
    11941 ttacctaaag aaaatgtcgc catgttactc atcctgtgga tcatgatatt caacgcacgc
    12001 ctgtagcgtg ttttcaatca gcgtggcaac cgtcatcggg ccaacgccgc cgggaacagg
    12061 cgtaatgtat gaggcgcgtt tagccgcgtc ttcaaacacg acgtcgccca caactttgcc
    12121 attttccaga cggttgatgc cgacatcaat cacaattgcg ccttctttga tccagtcacc
    12181 gggaataaag cctggcttgc caacggcaac gatcaataga tcggcatttt ctacgtgatg
    12241 acgcagattt ttagtgaagc ggtgagtcac tgtagtggtg caacctgcca gcagcagttc
    12301 catgctcatc gggcggccaa cgatattcga tgcgccaatc accacggcgt tgaggccgaa
    12361 ggtatcaatg ttgtaacgct caagcagcgt gacgataccg cgcggggtgc agggacgcag
    12421 acgcggcgcg cgctggcaca gacgaccgac gttgtaagga tggaaaccgt ccacgtcttt
    12481 gtccggatga atacgttcca gcactttgac gttatcaata cccgccggta acggcagttg
    12541 aaccagaatg ccatcgatgg tgttgtcggc attcagcgta tcgataagct ccagcagctc
    12601 cgcttcgctg gtggtttccg ggaggtcata agagcgggag acgaacccga cttcttcaca
    12661 agccttgcgt ttgcttgcga cataaatttg cgatgcaggg ttactaccca ccagcacaac
    12721 ggccagtcct ggtgcccgca gtccggctgc aatacgcgcc tgaacttttt gagcaacttc
    12781 agagcgcacc tgctgcgcaa tcgttttacc gtcaataatc tttgctgcca tcagagagag
    12841 gattccatct gttacgtagg tcgaagggga tgcgcctatt ttgtcagaag cggggcgcgc
    12901 tgtcaggttt cgtttcagat ttatcgcgtg aagcgacctc ttgcgaaggt gaggcgcacc
    12961 gtcgctgaga ctgaaagctt catttttcgt ccatgatggc gttgtaaatc tggaactgat
    13021 ttatttcctt gtctaaggat taagataatt taagaaatac ctgacaatat aaaaagaatt
    13081 ttcagcctgg taatttaccg cttcaggtct atatttgtgt tgaatatatt atgcgcggaa
    13141 gtattcat
//
LOCUS       DAPHJE010000118        13148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPHJE010000118 DAPHJE010000000
VERSION     DAPHJE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.119"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(152..364)
                     /gene="cspH"
                     /locus_tag="RH222_003590"
     CDS             complement(152..364)
                     /gene="cspH"
                     /locus_tag="RH222_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV3798552.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            650..862
                     /gene="cspG"
                     /locus_tag="RH222_003591"
     CDS             650..862
                     /gene="cspG"
                     /locus_tag="RH222_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV3798553.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            873..1061
                     /locus_tag="RH222_003592"
     CDS             873..1061
                     /locus_tag="RH222_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV3798554.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1036..1266
                     /gene="ymcE"
                     /locus_tag="RH222_003593"
     CDS             1036..1266
                     /gene="ymcE"
                     /locus_tag="RH222_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV3798555.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            1256..1429
                     /locus_tag="RH222_003594"
     CDS             1256..1429
                     /locus_tag="RH222_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV3798556.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1478..2551)
                     /locus_tag="RH222_003595"
     CDS             complement(1478..2551)
                     /locus_tag="RH222_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV3798557.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHVAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2623..5367)
                     /gene="torS"
                     /locus_tag="RH222_003596"
     CDS             complement(2623..5367)
                     /gene="torS"
                     /locus_tag="RH222_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV3798558.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5450..6478
                     /gene="torT"
                     /locus_tag="RH222_003597"
     CDS             5450..6478
                     /gene="torT"
                     /locus_tag="RH222_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV3798559.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6451..7143)
                     /gene="torR"
                     /locus_tag="RH222_003598"
     CDS             complement(6451..7143)
                     /gene="torR"
                     /locus_tag="RH222_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV3798560.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7273..8445
                     /gene="torC"
                     /locus_tag="RH222_003599"
     CDS             7273..8445
                     /gene="torC"
                     /locus_tag="RH222_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV3798561.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDMPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8445..10991
                     /gene="torA"
                     /locus_tag="RH222_003600"
     CDS             8445..10991
                     /gene="torA"
                     /locus_tag="RH222_003600"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV3798562.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10988..11587
                     /gene="torD"
                     /locus_tag="RH222_003601"
     CDS             10988..11587
                     /gene="torD"
                     /locus_tag="RH222_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV3798563.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11680..11985)
                     /gene="cbpM"
                     /locus_tag="RH222_003602"
     CDS             complement(11680..11985)
                     /gene="cbpM"
                     /locus_tag="RH222_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV3798564.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11985..12905)
                     /gene="cbpA"
                     /locus_tag="RH222_003603"
     CDS             complement(11985..12905)
                     /gene="cbpA"
                     /locus_tag="RH222_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV3798565.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3191 a         3327 c         3449 g         3181 t
ORIGIN      
        1 ctgttaaatg gcatttgtaa ctattaggtg cagagatatt cgcttaaatg gattagtttc
       61 tttgtggaaa agtaactgat gttataatgt atgatgttga ctattgcact attccaataa
      121 aaccagtcag ctttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg
      181 gtcctcgcag gccatttacg cggcaaaatt ccacgcgtaa tcctggtata agcacttctg
      241 cgtcgcgggg agtgaatgcg gaaatatgga cctggacttc tttacgaccg tcggagggga
      301 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
      361 acaaacaaat tccttactga aaatactgtg ccgcactata cggggttaat aaaataaagc
      421 cagcgatatt taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg
      481 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
      541 tctcattatc aggcattatc cctttctttt gagtctctct cctgaacact aagtagtttc
      601 tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa
      661 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
      721 tgatggcagc aaagacgttt tcgtccattt caccgccatt cagagcaatg aattccgcac
      781 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      841 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      901 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      961 tgggcgctaa gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc
     1021 aaatacgcac ggaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
     1081 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
     1141 ttgattattg aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg
     1201 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
     1261 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgct gactttatcg cgcaaaaaat
     1321 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga
     1381 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
     1441 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
     1501 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
     1561 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
     1621 acaacgcaaa agctttttta tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg
     1681 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1741 ttaatttatc taccgggatc cgtgatgggc aattttttgc gcatttgcca cagtcgatac
     1801 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1861 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1921 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttta
     1981 cgtccatcac cacgctgtag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     2041 ataacgcgat atagagaaaa aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     2101 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     2161 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     2221 tgatgacgat aaaagcgacc aataacagca tcgcggcgac gtggacggac ggtaactgac
     2281 ctgttgtaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     2341 ctcccggtct ggcgacaaat gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc
     2401 agaacgtgat ggcaatataa atattcattg ccaacagtaa taattgcgtc gctttacgcc
     2461 aggtcgtggc attacgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     2521 gctgccagcg ggttctttta ttctctgcca tgattttgcc agtccgttaa gttgtatacc
     2581 aaatgccact attctagttg ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     2641 tttcttatgc aaccatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2701 gggggcagat aatggctgct gctccagttg tgcgcatagc tggctggcga tacgcattcc
     2761 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctatcctg
     2821 actggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtg taaataatac
     2881 cagccattcg tggatcttct ccgtccccat taactgagca tcttcattga gttgcgatac
     2941 atccagcgat tgatcattat tgacttgcag ttggagatag tgcgccagta actgaccgag
     3001 cacttcacgc ggcaccggtt tagggataat cccgcggaat aatgaactgg tacgctggcg
     3061 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     3121 tgccagttgt cgggcaagcg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     3181 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc
     3241 aacaatctgc gcaccactgg ttttcagcat ctcaatggta attcgctggg ttagcgggtt
     3301 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg
     3361 cacgggtgcc gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     3421 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     3481 tcccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     3541 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     3601 atcagtgcga ctacgcagga taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa
     3661 caggttggtt ataacctgac gaatacgtcg tggatcgccc attaacgcgc acggcatatc
     3721 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3781 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgaccg aaacattctt
     3841 gccacctgct tcgatagcgg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3901 accagagtca gtaattgccc gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3961 cagtagctgt gcggtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     4021 cgccagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     4081 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     4141 gcggtggcga ttcagtgcat gaacattgct gcgaaacgca tccatcagcc gcccgatggt
     4201 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     4261 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg actgagcgat aaaccacgcg
     4321 ccagaggatc agaatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     4381 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgcgca gctcaatggt
     4441 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     4501 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     4561 atattggcta acggtagtta acgttgttgc gacctgcgca cgaacacccg gatcttcaat
     4621 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4681 cgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagggc
     4741 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4801 gagtgcactt tctgcggcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4861 ggtcgctccg gcggaggttg ccgcattatt cgcctgacct tgcgccaggc gtgcgatctc
     4921 atcggcggca gcgactatct gctgactgag ttgctgttgt tgctggcgca gttgcaaacg
     4981 ctgccccacc agttcccctt gctgacgtaa cgaacgggag atctcctgct cctgttgttc
     5041 aatagcggtg gtatcaaaac cttgttcccg taacgcttgc agcaacgcat taatcttcag
     5101 gctttgtgcg gtgagcattc gcccctgcgc ctgccacatc ttttcgttat cggcactggt
     5161 caggttctgc gcggcgaaaa gttcccaggc gctggcttcg ctcaactggc gcgccatatt
     5221 catggtagga atcaatgcct gagtattgtc tttttccacc tggctgataa agcgcaggtt
     5281 gtaccatccc accagggtac tggtcagggt taacagcgcc atcagggcaa agcccatcca
     5341 gagtcttcgg gtcagggtta aattcacggt cggtgcactt taggtgaaaa aggttgagtc
     5401 gcaaagcgga atgcatctag cataaagcct tattattgat gaggctatca tgcgcgtact
     5461 gctattttta cttctttccc ttttcatgtt gccggcattt tcggctgata acctgttgcg
     5521 ctggcatgat gcgcagcatt tcacggtgca agcctctacg ccgcttaaag ccaaacgcgc
     5581 atggaaactg tgcgcgcttt atcccagcct gaaagattca tattggttat cgttgaacta
     5641 tggtatgcag gaggctgctc gccgctacgg tgtggattta aaagtgctgg aggcaggcgg
     5701 ctacagccag ttggctaccc agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga
     5761 ggccattttg ctcggtagta gcacgacctc atttcccgac ctgcaaaagc aggtagcaag
     5821 tctgccggtg atcgaactgg taaatgctat tgatgctccc caggtgaaaa gccgcgttgg
     5881 tgtgccctgg tttcagatgg gctatcaacc ggggcgatat ctggtgcaat gggcgcacgg
     5941 taaaccactg aatgtgctgt tgatgcccgg acccgataac gccgggggca gtaaggagat
     6001 ggtcgagggt tttcgcgcag ccattgccgg aagcccggtg cgtattgttg atattgcgct
     6061 tggtgataac gatattgaaa tccagcgtaa cctgttgcag gagatgctgg aacgccatcc
     6121 agaaatcgac gtcgttgccg gaacggccat tgcggcagag gcggcaatgg gggaagggcg
     6181 taacctgaaa acgccgctta ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg
     6241 gctgaagcgg ggaagagtga ttatggctgc cagcgatcaa atggtctggc agggggaact
     6301 ggcagttgag caggccatca ggcaattaca ggggcaatcg gtgtctgata atgtcagccc
     6361 accgatttta gttctgacgc cgaaaaatgc cgaccgagaa catatccgcc gctcgctgtc
     6421 accgggggga tttcgtccgg tctattttta tcagcacaca tcagcggcta agaaataacc
     6481 ttcaccatgt tgcgtcacca gtaaatccgc gctgagttta tgacgtaaac gacgaattaa
     6541 cacatcgacg gtgcgcaggt cagggttttc cacccgacgc gcagaaagca tacgtagcag
     6601 acgttcacgg ctgagaattt cgcccggatt cgtcacaaat gccaccaaca tttcatactc
     6661 tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc gtatggcgcg acacattcag
     6721 gcaataaccg gcaaagcgat agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag
     6781 gtcgattcgc cagagcagat ttttcacccg tactaccagt tcgcgcagtt ccagcggttt
     6841 ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata cggtcaatcc gatcgctgcg
     6901 tccggtaacc agaataatcc ccaccgttga gcgttctcgc agggcgcggg ttaacatcag
     6961 gccattttca tcgggtaagt tgatatccag cagaattaaa tctaccggct gattctgcat
     7021 aatttcccgc agcccggcac cgctcgctgt aacggaaacg gtatacccct cctgagtgaa
     7081 gtaagattgt aatcgcgcct gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg
     7141 catcagaggg ttttactcat tctgttcata tctgttcata ttctgccgta agccgttcat
     7201 cctgaccagt gccgctgttc atatttgctc attaagatcg cttcactaaa ccataatttt
     7261 acaggggcta ttatgcggaa actctggaac gcgctacgcc gacccagtgc tcgttggtcg
     7321 gtactggcgc tggtcgcaat tgggattgtg attggcattg cgctgattgt attgccacac
     7381 gttgggatca aagtcaccag cacaaccgaa ttttgtgtca gttgccacag tatgcaaccg
     7441 gtgtatgaag aatataaaca gtcggtgcat ttccagaacg cctccggcgt gcgagctgaa
     7501 tgccatgact gtcatatccc gccggatatg ccaggcatgg tgaagcgtaa actggaagcg
     7561 agcaatgata tctaccagac ctttattgct cactccattg atacacctga aaaattcgaa
     7621 gccaaacgcg cggaacttgc cgagcgtgaa tgggcgcgaa tgaaagaaaa caactcggca
     7681 acctgccgct cctgccataa ctacgatgcg atggatcatg cgaagcagca tcctgaagcg
     7741 gcacgtcaga tgaaggtggc agcgaaagat aatcaatcct gcatcgactg tcataaaggt
     7801 attgcccacc agttaccgga tatgagtagc ggcttccgta agcagttcga tgagctgcgc
     7861 gccagtgcta atgacagtgg tgacacgctg tactctattg atattaagcc aatttatgcg
     7921 gcgaaaggcg ataaagaagc ctctggttct ctgctgcctg cttcggaagt gaaagtcctt
     7981 aaacgtgacg gcgactggct gcaaattgaa attaccggct ggacggaaag cgccggacgt
     8041 cagcgtgtac tcacccaatt cccaggtaaa cgcatctttg ttgcctcgat tcgtggtgat
     8101 gtgcagcagc aggtaaaaac gctggagaaa accaccgttg ccgacaccaa taccgagtgg
     8161 agcaagttgc aggccactgc gtggatgaag aaaggcgaca tggtgaacga tatcaaaccg
     8221 atctgggctt atgcggattc gttgtacaac ggcacctgta accagtgcca cggcgcaccg
     8281 gaaatcgccc actttgacgc taacggttgg atcggcacgc tcaacggcat gattggcttt
     8341 accagtctcg ataaacgtga agaacgcacc ttgttgaaat atctgcaaat gaatgcgtct
     8401 gacaccgcag gtaaggctca cggcgataag aaggaagaaa aataatgaac aataacgatc
     8461 tctttcaggc atcacgtcgg cgttttctgg cacaactcgg cggcttaacc gtcgccggga
     8521 tgctggggcc gtcattgtta acgccgcgcc gtgcgactgc ggcgcaagcg gcgactgacg
     8581 ctgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc cgcgcgacgg
     8641 tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat ccgtcgaaaa
     8701 tgattgccgg attgccggat cacgtacaca acgcggcgcg tattcgttat ccgatggtac
     8761 gcgtggactg gctgcgtaag cgccatctca gcgatacctc ccagcgcggt gataaccgtt
     8821 ttgtgcgcgt gagctgggat gaagccctcg acatgttcta tgaagaactg gaacgcgtgc
     8881 agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatcg acggggatgt
     8941 tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat agcgttggta
     9001 cgggcggaga ttactctacc ggtgctgcgc aggtgatcct gccgcgcgtg gttggctcaa
     9061 tggaagtgta tgaacagcaa acctcctggc cgctggtatt gcagaacagc aaaaccattg
     9121 tgctgtgggg ctctgatttg ctgaaaaacc agcaagcgaa ctggtggtgc ccggatcacg
     9181 atgtttatga atattacgcg cagctaaaag cgaaagtcgc cgccggtgaa attgaggtca
     9241 tcagcatcga tccggttgtc acatccaccc atgagtatct ggggcgcgag catgtgaagc
     9301 acattgcggt taacccgcaa actgatgtgc cgctgcaact ggcgctggcg tatacgctgt
     9361 acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtgggt tttgagcagt
     9421 tcctgccgta cctgctgggt gagaaagacg gtcagccgaa agatgccgca tgggctgaaa
     9481 aactgaccgg cattgatgcc gaaaccattc gtgggctggc gcggcagatg gcggcgaaca
     9541 gaacgcaaat tattgctggc tggtgcgtgc agcgtatgca gcacggtgaa cagtgggcgt
     9601 ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg cctgccaggt ggtggctttg
     9661 gttttggctg gcactataac ggcgcaggca cgccggggcg taaaggcgtt attcttagtg
     9721 gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgattac aaaggctaca
     9781 gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg aaagtgatca
     9841 actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt gccggaacta
     9901 acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc aagctggaaa
     9961 cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat atcgtgctgc
    10021 ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac tccaaccgtg
    10081 gcattatcgc catgaaacag gtggtgccgc cgcagttcga ggcgcgcaac gactttgata
    10141 ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc ctttaccgaa gggctggacg
    10201 aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa ggacgcggcg
    10261 ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag tttgaccatc
    10321 cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa ccgctgggca
    10381 cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac tacgacgatt
    10441 gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg cccggctcgc
    10501 agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac tcgcagttat
    10561 gtgagtcgga aactctgcgt cagcaatata cggtagcggg taaagagcca gtgttcatta
    10621 acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc gtctttaacg
    10681 ctcgcggtca ggtgttggca ggggcagtag tttctgaccg ctatgcaccc ggcgtggcac
    10741 gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt gcactgtgca
    10801 aatacggtaa ccccaacgtg ctgaccatcg acatcggtac ttcgcagctg gcgcaggcga
    10861 ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggagcagtg gagcaggtga
    10921 cggcgtttaa cggccctgtg gagatggtgg cgcagtgcga atatgttccc gcgtcgcagg
    10981 tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct ggctggcgca
    11041 gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg cgcagatggc
    11101 tgaatggttt tcgttgctga aaagcgaacc gccgctggct gcggcggtga atgagctgga
    11161 aaactgtatt gccacgctga cagtacgtga cgatgcccgt ctggaactgg ccgctgactt
    11221 ttgcggcctg tttctgatga ccgacaaaca agcggcgctg ccgtatgcat cggcctacaa
    11281 acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaaa ccagcggcaa
    11341 tttcaacgaa ccggcagatc atctggcgat ctatctcgaa ttgctcagcc atctgcattt
    11401 ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac agtttgcggc aaaaaacact
    11461 gacggcgctg tggcaatggt taccagagtt tgttgtgcgt tgtcgtcagt atgacagctt
    11521 tggtttttac gcggcactaa gccagttatt gctggtgtta gtggagagcg accaccaaaa
    11581 cagataacgt cgtttgtgcg cctgaaaaga cgcgtttagc gtcgcatcag gcattatggg
    11641 gcagttgccg gatgcgtcat cacatccggc aaacacacat cacggatgag ctacaaaccg
    11701 ggaaagtcgc tggcgcagca ggcggttttc ctgcttcagg tgcgcaatat catccattaa
    11761 cgtcagcgcc accgcgatcc caggccagtc cagagccagt tcatgacgca ggcgtactgc
    11821 gcgttgcacc acgatggcgg catggtcgtc aaatacccag gttgtttcct ggatctcacg
    11881 cggttcaacc acccccaaac cgacaatttc attcaactcc tcttcagaga tgccggtatg
    11941 caggcaaaat tcggtaatag taaaagtcac cgtaacatta gccattatgc tttcccccaa
    12001 tctttacgtg gatcaaaaga cgactgggcg tctgccagtt gctgccacag cgcggcagtg
    12061 ttttcatccg gtttcggcgg catcacgatt ttcagtaccg catacagatc gccggtctgt
    12121 tttttgctca ccagaccttt gcctttaacg cgcaatcgtt gcccggcctg gctgcctggc
    12181 gggatagtca gcaaaatgct ttctttcagt gttggaacgg tgactttagc acccagcgcc
    12241 gcttcccacg ggctaaccgg cactacaatt tccagatcat ggccgacaat atcgaacagc
    12301 ggatgtggcg caatatgaat caccagccac aaatcgccat ttggaccgcc gttttcgccc
    12361 ggcgtcccct ggcctttcag acggatgcgt tgaccattgc cgacgcccgc cgggatcttg
    12421 acattcagcg ttttcggaat ttcctgttcg atcatgccaa aggcgttata aaccggcagg
    12481 ttatagctga tggtacgctt atgctcagta agcgtttctt cgaggaatac cgccacttcg
    12541 atttcaatat cgtggccgcg tgtggcgggg cgttgacggc tctggcgggc atgctgaccg
    12601 aaaattgacg agaagatatc gtcaaaatct tcggcgttaa aactctgacc gtcgctatga
    12661 tggaactgac ggttaaattg cggatcgttg cgatgttgcc acatctgatc atactcagcg
    12721 cgacgttgtt catcacttaa cacttcccag gcttcagcga cctctttgaa gcgggcttcg
    12781 gcatccggtt ctttgctgac atcaggatgg tatttgcggg ccagtcgacg ataggcggtc
    12841 ttgattgtct tgagatcgtc cgtcggtttc acgcccatga tggcgtaata atcctttaat
    12901 tccatagcgt tatctcgcgt aaatcaacac aaattgaagg aacccctgta aggtaactcc
    12961 tataagtgta gggtaatcct caaaatttca tatgccaaca cagaatatgt tattgaaatc
    13021 atcgcggaga ggaggtcgcc tcaaggtggg gtgctgaaca tattttaaac aggtgaaaaa
    13081 gggtgagcga tttttgatag tggaaccagg cactttaagt ttaactgggg cgtcattatt
    13141 tattaaat
//
LOCUS       DAPHJE010000119        12761 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPHJE010000119 DAPHJE010000000
VERSION     DAPHJE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.120"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..214
                     /locus_tag="RH222_003604"
     CDS             44..214
                     /locus_tag="RH222_003604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798566.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(272..1045)
                     /gene="zupT"
                     /locus_tag="RH222_003605"
     CDS             complement(272..1045)
                     /gene="zupT"
                     /locus_tag="RH222_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV3798567.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            1188..1976
                     /gene="ygiD"
                     /locus_tag="RH222_003606"
     CDS             1188..1976
                     /gene="ygiD"
                     /locus_tag="RH222_003606"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV3798568.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQVG"
     gene            complement(2014..3174)
                     /locus_tag="RH222_003607"
     CDS             complement(2014..3174)
                     /locus_tag="RH222_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV3798569.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(3180..3851)
                     /locus_tag="RH222_003608"
     CDS             complement(3180..3851)
                     /locus_tag="RH222_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV3798570.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(3999..5480)
                     /gene="tolC"
                     /locus_tag="RH222_003609"
     CDS             complement(3999..5480)
                     /gene="tolC"
                     /locus_tag="RH222_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV3798571.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            5685..6314
                     /gene="nudF"
                     /locus_tag="RH222_003610"
     CDS             5685..6314
                     /gene="nudF"
                     /locus_tag="RH222_003610"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV3798572.1"
                     /translation="MLKPDNLPVTLGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6315..6737
                     /locus_tag="RH222_003611"
     CDS             6315..6737
                     /locus_tag="RH222_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV3798573.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            6762..7589
                     /gene="cpdA"
                     /locus_tag="RH222_003612"
     CDS             6762..7589
                     /gene="cpdA"
                     /locus_tag="RH222_003612"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV3798574.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            7589..8170
                     /gene="yqiA"
                     /locus_tag="RH222_003613"
     CDS             7589..8170
                     /gene="yqiA"
                     /locus_tag="RH222_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV3798575.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            8199..10091
                     /gene="parE"
                     /locus_tag="RH222_003614"
     CDS             8199..10091
                     /gene="parE"
                     /locus_tag="RH222_003614"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV3798576.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(10136..11005)
                     /locus_tag="RH222_003615"
     CDS             complement(10136..11005)
                     /locus_tag="RH222_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
                     /protein_id="HDV3798577.1"
                     /translation="MNNKFKIILWMMLSALPALSYAQVKNIVTGEYSAVSKIITEKET
                     LYPSDKILLVFDIDNTLLTSGTRIGGDIWYQWQTDKLPLKPDDSQKVPCLYENTISML
                     YELSPMQLTEPQLPTMLKQWQQKHTAFALTSRAPDTLFPTLRELKRNGIDFSSSALRK
                     KEDTLPPFEKGKLKRSWLYSQGVFFSSGQDKGVILDYMLDKMGNKYDAIVFIDDGQAN
                     IHAMTKMFSKDKWFSTDFTIIHYTRVEDELIKQQGAVITTAQAEKMATDWKTLAGSLA
                     TLFPDRNKLCPIK"
     gene            complement(11019..11714)
                     /locus_tag="RH222_003616"
     CDS             complement(11019..11714)
                     /locus_tag="RH222_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV3798578.1"
                     /translation="MLSCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSN
                     GIGLMGELRYNTDEGDKDKWAPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQ
                     YWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQ
                     KTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKIGSWIPYMSIADYKGVDKTSSN
                     RQIRWRWGLTYTF"
     gene            complement(11849..>12761)
                     /locus_tag="RH222_003617"
     CDS             complement(11849..>12761)
                     /locus_tag="RH222_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV3798579.1"
                     /translation="GDASLYTLKIQVSSRGIEQVNFLNQRPLLDAKQQPFVTNTVDAE
                     GLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNISSDKESSNGIRSGN
                     AWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTARLLQFSIDADGRPSKQLHEYL
                     YITDPVPQVSKFGINDNGVSEVLALNDHQLLVIERSGRNVSAGFNDWDYSVRVYMVDL
                     TAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGVTFGPLIDGHTSLI
                     FVSDNNFQPHQQTKFYLFIDKENKLKI"
BASE COUNT         3117 a         3069 c         3286 g         3289 t
ORIGIN      
        1 atgcgctaaa tgtttagtat tgttaaatat taagggtaaa aggatgttta tcgcctggta
       61 ctggattgta ttgattgctc ttgttgtggt gggttatttc ctgcatttga aacgttattg
      121 tcgggcgttt cgccaggaca gagacgcact gcttgaagcg cggaacaaat acttaaacag
      181 tacgagagaa gagacagccg aaaaggtaga ataaggcttt agtgaagcta gcagaagttg
      241 tccatgcgct ggccaacttc tgatgttgct tttaaccaat tcccgccgtt tgtagcagca
      301 ctaaactgaa tcccatcact gacattccac atagtacgcc atagctgggg ttattattag
      361 ggtcaatctc tttggcgagc ggcattaatt catcaaccga gagcgccacc ataattcctg
      421 caaccgccgc catgattgcc gccatgacca cgggggaaat catgctaccg aggattaacc
      481 acgccagcac accaccaaga atttctgcca atccagaaat ccccgcccac agaattgccg
      541 tgcgtttaga acccgttgct gcataaaccg ggcctgcaac cgccagacct tcaggaatat
      601 tgtgcaacgc gacggccagt gcgatgccaa atcccagctc caggttgctg ctcgccgtga
      661 caaaggtggc aatcccttcc gggaagttat gcagactgat gccgagagtg agcagaatgg
      721 ctgtgcgctt gatcgatttt ggcaacggct gcaccgattt ttgcattaaa tcctgcggat
      781 gcgcatgtgg caacatgcgg tccaggccaa aatagccaag cagaccgaag ataaacattc
      841 cataacccaa cacaggcgac attccttcag ctgctagtgc ggcaggaagc atttccatta
      901 atgagatgag caacatgatc cccgccgcaa atcctagcga aaacgccagt aagcggttcg
      961 agggtttttg cccgagaacg ccgagaaacg cgccaataaa cgtggctgcc cccgccagta
     1021 tggtcagaat gagaggtact gacatcgaca actccttata aatcttcacc ttcaggcaga
     1081 tcgtcaaaat aactgatgat catcatcatt gttatccggg ttcttactcc gccgaacaag
     1141 cgtattgtga ggatatcaaa atgacacctt tagttaagga tatcatcatg tcttcaacac
     1201 gtatgccagc attgttttta ggtcacggta gtccgatgaa cgtgctggaa gataatttgt
     1261 atacccgcag ctggcagaag ttggggatga cattgccacg cccgcaagcg attgtggtgg
     1321 tttcggctca ctggtttacc cgtggaacag gagtgaccgc gatggagacg ccgcccacga
     1381 ttcatgactt tggtggcttc ccgcaggcgc tgtacgatac gcattatcct gctccgggtt
     1441 cgcctgtgct ggcacagcat ctggttgagc tgttagcgcc ggtcccggtg acgctggata
     1501 aagaagcctg gggctttgac cacggttcct ggggcgtgct gattaagatg tatcctgacg
     1561 ccgatatccc gatggtgcag ttgagtatcg acagtagcaa acctgccgcc tggcatttcg
     1621 aaatggggcg caaactggca gcgctgcgag atgaaggaat aatgttggtc gccagtggca
     1681 acgtggtgca taacctgcgc acagtgaagt ggcacggtga tagttcaccg tatccgtggg
     1741 cgacgtcgtt taatgagtat gtgaaagcga atctgacgtg gcaagggcca gtggaacaac
     1801 atcctctggt gaattacctc gaccatgaag gtggcacgtt atcgaaccca acgccagagc
     1861 attatctgcc gttgttgtat gtgttaggtg cgtgggatgg gcaggagcca attaccattc
     1921 cggttgaggg tatagaaatg ggcagcctga gtatgctgtc ggtgcaggta ggttaacaat
     1981 taccggatgg cgaatgccat ccggtatcat tgattattca acaaaaatat gtggataaaa
     2041 ccgagacata tcctgggtga tcaatgcacg gtcttcacga atgccaattc cggcaggttg
     2101 atcgttcacc agccagctac caatcagcat atagctgtcg ccgaatttcg gtaacgggtg
     2161 gaattgctga acaatcattc cttcttcgcc atacggacct tccgctgctt caatggtttt
     2221 gccgttctca atgatcgaca cgtttgcgcc ttcacgggag aagatcggct taaccacata
     2281 tttttccatt tgcggatgat catcttccgc aaaataagcg ggcagcaggt tcgggtgatt
     2341 cgggaacatc tcccacaaca gcggtagaag tgccttgttg gagataatgc tcttccacgc
     2401 cggttccagc cagcgtacgc ctgcatcctc cagcttggtg gagaacatct cacgcagcat
     2461 aaactcccac ggatacagct tgaacaggtt ggaaattacc tgatcctgta aatccgtgaa
     2521 ctgacctttt tcacctaacc cgatatcatc gatgtagagg aactcagtag caatttcagc
     2581 ttccgttgcg cagtcctgca aatactgaat ggttccgcga tcttccaccg tgtcgcgaca
     2641 gcaggtgaga tgcagcaact ggaagccata ctgttcacgc agctcaacga agcgatcgat
     2701 cagtttttct tgcagactgt taaactggtc gctgccctcc ggcaagttac cggcgttaag
     2761 ctgatcttcc agccagatcc actgaaagaa cgccgcctcg tatagtgacg ttggcgtatc
     2821 ggcgttattt tccagaagtt taggttcacc agtgccatcc cacgccagat caagacgcga
     2881 ataaagcgat ggctggtgcg tcagccatga ctggcgcaca aaactccagg tgtgttttgg
     2941 aatgcggaat ttggtcatca gctcatcgct ggcgatcact ttttccacca ctttcaggca
     3001 catctggtgc agttcggcgg tgacttcttc cagcttttca acctgggcga gggtcaactt
     3061 gtagtaagca tcttcacacc agtacggctc gccgtacatg gtgtgaaaat tgaaaccgta
     3121 ttcgtgggct ttctcacgcc agtccgggcg ctcggtaata ctgactcttt ccatcggtat
     3181 cagccaccca ttgaacgaga agaggtaccg gttgcactac gctgcatagt gctttgtttg
     3241 gcaacagatt caccaaaacc gccacgggta acggtagtgg tggtcgccgg ttttggtgcc
     3301 attgccgtct tcggtacggt catggtgcgg cctggctggg ctgcgccata gtttttaccc
     3361 gtcgcgtcgg tatatttacc gtaagccgga ctggctgggt ttttcgagga gaacagcggc
     3421 tgctgtgcaa atcccgcgcc gccgcccatc agacgcccca tcatgtaacc ggccatcagc
     3481 ggcatccaga aactcccgct ggattgctgg gcctgcgcct ggttttctgg tgccatgcca
     3541 gcctgggctg gtgcctgctg gcactgacct tcaccaaatt cagcaacaca gtcttcacgg
     3601 gtggcgtatt tcggcgcagt acgttcggct tctttcagcg cattgttgta cgcggtggta
     3661 cattcggcgc ttttgcctgg gtttgcagct gaacagtcgt cagcattttg atagagagac
     3721 actgtttcat cactcttttc acagccagcc agcataaaaa cagtggcaac cgcgagagcg
     3781 actggtgtca gatggcgtgc gctccagttt ttgcggaacg atgcgtggcg tatggatttt
     3841 gtccgtttca tttttgtctt ccgggaccag tggtaaatac ccatcagaat agaggatggc
     3901 tggtcgaaat tgaagcgaga agggggaaga atgcggcaga taacccgtat ctttacgttg
     3961 ccttacgttc agacggggcc gaagccccgt cgtcgtcatc agttacggaa agggttatga
     4021 ccgttactgg tggtagtgcg tgcggatgtt tgctgaacga cgggggccgg gctatcaggc
     4081 gcataaccat cagcaatagc attctgttcc ggcgtttgcg gggcaacgtt ttccggatta
     4141 gtggaaaccg gtttgctcag cgcattgttc agtgccagca gatcctgctc gttcaacgta
     4201 cccagggctg acttaatatt cagctgatta atcaggtagt tataacgcgc attcgccagc
     4261 tcttgcttgg cgttgtacaa cgtggtggtc gcatccaaca catcaacaat ggtacgcgta
     4321 ccgaccgagt agcccgcttc catcgcgtct aatgagcttt gagcggaaac tacggcttgt
     4381 ttgtaggcgt taatgctact gatagatgca ttaatgttgt tgaaggagga acgcacggtc
     4441 tgcacgacgc tacgatgggc actttccagt tgctcgctgg caccgacaaa gttgtactgt
     4501 gcctgtttca cctgcgagtt aaccattccg ccctgataaa tcggcagcga gaagctcagg
     4561 ccaactttgt tctggcccat attgctatcg tcatactggg taccagcggc accacgggtt
     4621 ttcgaaccgc tataagaggt gtcagaaatc ccggtagaag ccgttaaatc cagagtcggt
     4681 aagtgaccat cctgcgcctg gcgaatttgc tcgcgcgcca ggtcctggct caagcgtgcc
     4741 tgtaacagcg acaggttgcg tttttcggct tctttcagca gcgcgttaac cggctgtggt
     4801 ttgtcggttt taaagttttc gacattcagc gcagccagtt ccggatagta gttaccggtg
     4861 atctggcgca gctgctctac cgcgttatca aggttattac gtgcggtcac ttcgttcgcc
     4921 agcacggtat cgtactgtgc gcgggcgttc tgcacgtcgg tgatcgctac caggcccacg
     4981 ttaaaacgtt gggtggtttg atctaattga cggtagatcg cttctttttg tgcctgtgta
     5041 taggaaagaa cgtcaatagc attcaacacg ttgaaataag cggtcgcggt gttgaggatc
     5101 aaggtttgct gatcggtctg atacgtgacg tcctgaatcc ctgctgcttt ttcctgcagc
     5161 gttaacgcac gccatttcga catatcaaaa atggattgag ttaactgcaa ggacgcactg
     5221 gtcgcgttag agttgatgcc gttcgcgtcg cggtagccgt tgctataggt gtaatctgca
     5281 cctaaaccta gctgtggcag taatggactg cgcgcttcat taattttttc aaaggcagca
     5341 tcacgatcgg cggcagactt acgcaattcc gggttactaa ggcgtgcttg ctgataaact
     5401 tgcatcaggt tctcggcctg gctcaacgaa ctgaacccag aaaggctcag gccgataaga
     5461 atggggagca atttcttcat ttgcattcct tgtggtgaag cagtatttag cgcgatcaaa
     5521 ctgtaaaatt attgcggatt ctagcagaag ccgctaccgc aaaaggttgg cgttacgtgc
     5581 catagggcgt taatgtgcct caatttaaca catgactgct caaacggcag acaaacgtcg
     5641 ctgaaattca catttaattc actattagtg ccaggacatt aacaatgctt aagccagaca
     5701 acctgcccgt tacacttggc aaaaacgatg tagaaattat tgcacgagaa acactttatc
     5761 gcggcttttt ttcattagat ctttatagat ttcgtcatcg tttattcaac gggcaaatga
     5821 gtcatgaggt acggcgggaa atttttgagc gcggtcacgc cgcagtcttg ctaccctttg
     5881 acccagtgcg tgatgaagtt gtgctgattg agcagattcg gattgccgca tacgacacca
     5941 gtgaaacccc ctggctactg gagatggttg ccgggatgat tgaagagggt gaaagtgtgg
     6001 aagatgttgc ccgtcgcgaa gcgattgaag aggcgggact gatagtcaaa cggaccaaac
     6061 cggtgttaag tttcctggca agcccgggag gcaccagtga gcgttcgtca attatggtgg
     6121 gcgaagtgga cgccacgacc gcaagcggta ttcatggtct ggctgatgaa aacgaagata
     6181 ttcgcgttca tgtggtaagc cgggaacagg cataccagtg ggtagaagag gggaaaatcg
     6241 acaacgcagc gtcggtcatc gctttgcaat ggctgcagct gcatcatcaa gcgttaaaaa
     6301 atgagtgggc ataaatgaag cgttacacac ctgactttcc tgaaatgatg cgcctgtgcg
     6361 agatgaactt ttcacaattg cgccgtttgt taccgcgcaa tgacgcaccc ggcgaaactg
     6421 taagctatca ggtggcaaac gcacaatatc ggctgacgat tgtggaatcg acccgataca
     6481 ctaccctggt gacaatagaa cagactgcgc ccgcgatcag ttactggagc cttccgtcaa
     6541 tgacggtgcg tctgtatcat gacgcgatgg tggctgaagt gtgttcaagc cagcagattt
     6601 ttcgcttcaa agcgcggtat gattatccta ataaaaagtt gcatcaacgc gacgaaaagc
     6661 atcaaattaa tcagttttta gcggactggt tgcgatactg tttagcacat ggagcgatgg
     6721 cgattccggt ttattagcgt cgtgaaacct aaggacacca tttggaaagc ctgttaaccc
     6781 ttcctctggc tggtgaggcc agagtcagga ttttacaaat taccgatact cacctgtttg
     6841 cacaaaagca cgaagccctg ttaggggtaa atacctggga aagctaccag gcggtgctgg
     6901 aggcgattcg tccacaccag cacgaattcg acctgattgt cgcgacaggt gatttagcgc
     6961 aggatcaatc ctctgcggcc tatcagcatt tcgctgaagg catcgcaagt tttcgtgcgc
     7021 cctgcgtctg gctgcctggc aaccacgatt tccagcccgc gatgtacagc gcgttacagg
     7081 atgcgggtat ctccccggcg aagcgggtgt ttattggtga gcaatggcaa atcctgttgc
     7141 tggatagcca ggtgtttggc gtgccgcacg gtgagctgag cgagtttcag cttgagtggc
     7201 tggaacgtaa actggccgat gcgccagaac gccatacgtt gctgctgctg catcatcatc
     7261 cgctacctgc gggttgtagt tggctcgatc aacacagtct gcgtaacgcg ggcgaactgg
     7321 ataccgtgct ggcgaagttt ccgcacgtca aatacttgct gtgcggtcat attcatcagg
     7381 agctggatct cgactggaac ggtcgccgcc tgctggcaac gccgtcgacc tgtgtgcagt
     7441 ttaagccgca ctgttccaac tttacgctgg atactatcgc gcccggctgg cgtactctcg
     7501 agttacatgc tgatggcacg ctgaccaccg aggtgcatcg cctggcggac acacgtttcc
     7561 aacctgatac cgcttcagaa ggctactgat gtctacgctt ctttatttac acggtttcaa
     7621 cagctcgccg cgctctgcaa aagcgagctt gttaaaaaac tggctggcgg aacatcaccc
     7681 tgacgttgag atgatcattc cgcagttgcc gccgtatcct tccgacgcgg cagagctgct
     7741 ggaatccatt gtcctggaac atggcggcga ttcgctgggt attgtcggtt cgtcactggg
     7801 gggatattac gccacctggt tgtcacaatg ttttatgctg cccgcagtgg tggtaaaccc
     7861 ggcggtgcgc ccgtttgaac tgctgacgga ctatctcggt cagaacgaga acccctacac
     7921 cgggcagcaa tatgtgctag agtcacgcca tatttacgat ctcaaagtca tgcagattga
     7981 cccgctggaa gcgccggatt tgatctggct gctgcaacag acgggagatg aagtgctgga
     8041 ttaccgccag gcggtggcgt actacgcttc ctgccgccag actgtcatag aaggcggcaa
     8101 ccacgcattc acgggcttcg aagattattt caacccgatc gtcgattttc ttggtctgca
     8161 ccatctctga cgatcaactc gaattactaa cttaaaccat gacgcaaact tataacgctg
     8221 atgccattga ggtactcacc gggcttgagc cggttcgccg ccgtccgggg atgtataccg
     8281 ataccactcg ccctaaccat ttggggcaag aagttattga taacagtgtc gatgaagcac
     8341 tggcgggtca cgcaaaacgc gtggatgtaa tcttacatgc tgaccagtcg ttagaagtta
     8401 ttgacgatgg gcgcgggatg ccggtggata ttcacccgga agagggtgta ccggcggttg
     8461 aattgattct ttgccgtctg catgcaggcg gtaaattctc taacaaaaat taccagttct
     8521 ctggcggcct gcatggcgtg gggatttcgg tggttaacgc cctgtcgaag cgcgttgaag
     8581 ttaacgtgcg ccgcgatggt caggtttata acatcgcctt tgaaaatggc gaaaaggtgc
     8641 aggatttaca ggttgtcggc acttgcggta aacgcaatac cggtaccagc gtgcacttct
     8701 ggccggatga aaccttcttt gacagcccgc gattttctgt ttcacgcctg acgcatgtgc
     8761 tgaaagccaa agcggtactg tgccccggcg ttgagattac ctttaaagat gagatcaaca
     8821 acaccgaaca gcgctggtgc tatcaggacg gtctgaatga ttacctggcg gaagcggtaa
     8881 acggtttacc gacgctgcca gaaaaaccgt ttatcggtaa tttcgctggc gatactgaag
     8941 ctgtggactg ggcgctactg tggctgccgg aaggcggtga actgctgacc gaaagctacg
     9001 tcaacctgat cccaacgatg cagggcggta cccatgttaa tggcctgcgt cagggcctgt
     9061 tggacgcgat gcgtgagttc tgtgaatatc gcaacattct gccgcgcggc gtgaagctgt
     9121 cggcggaaga tatctgggat cgctgcgcct atgttctgtc ggtgaaaatg caggatccgc
     9181 agtttgccgg gcagacgaaa gagcgtctct cttcgcgtca atgcgcggca ttcgtttctg
     9241 gcgtggtgaa agatgccttt atcctgtggc tgaaccagaa cgttcaggcg gcggagttgc
     9301 tggcagagat ggcgatttcc agcgcccagc gtcgtatgcg tgcggctaaa aaagtggtgc
     9361 gtaaaaagct gaccagcggc ccggcgctgc ctggcaaact ggctgattgt accgcgcagg
     9421 accttaaccg taccgaactg ttcctcgtgg aaggtgactc cgcaggcgga tctgccaagc
     9481 aggcgcgcga tcgtgaatat caggcgatca tgccgctgaa aggtaagatc cttaacacct
     9541 gggaagtttc ttccgacgaa gtgctggcct cgcaggaagt gcatgatatt tcggtagcga
     9601 tcggtatcga tcctgacagc gacgatctga gccagcttcg ttatggcaaa atctgtatcc
     9661 tcgcggatgc ggactctgat ggtctgcaca ttgccacgct actctgcgct ttgttcgtaa
     9721 aacacttccg cgcgttggtg aaacacggtc acgtttacgt cgcactgcca ccgctctacc
     9781 gtattgatct cgggaaagag gtttattacg cgctgacgga agaagagaaa gagggcgtac
     9841 ttgagcaatt aaaacgcaag aaaggcaagc cgaacgtcca gcgttttaaa ggtctggggg
     9901 aaatgaaccc gatgcaattg cgtgaaacca cgcttgatcc gaacactcgc cgtctggtgc
     9961 agttgactat cgatgatgaa gacgatcagc gtactgacgc gatgatggac atgctactgg
    10021 cgaagaaacg ctcggaagat cgccgcaact ggttgcaaga gaaaggcgac atggcggaga
    10081 ttgaggtctg ataatcttaa cccggcaaac atgtgttttg ccgggttgtc gttgattatt
    10141 ttattgggca cagtttgtta cgatctggaa atagtgttgc cagcgaacca gccagagttt
    10201 tccagtccgt agccattttt tctgcttgtg cagttgttat tactgcacct tgttgtttaa
    10261 taagctcatc ctccactctg gtatagtgaa taatggtaaa atccgtagag aaccatttat
    10321 ccttagagaa catcttcgtc atcgcatgaa tgtttgcttg cccgtcatcg ataaaaacaa
    10381 tcgcgtcata tttgttcccc attttatcca gcatataatc gagaattacg cctttatctt
    10441 gcccggaaga aaaaaatacc ccctggctgt acagccatga ccgtttgagt ttcccttttt
    10501 caaagggtgg caaagtatcc tctttcttcc tgagtgcgga agacgaaaaa tcaatgccgt
    10561 ttcgctttaa ttcccgcaat gtcggaaaga gagtatccgg tgcacgggat gtcagagcaa
    10621 aagcggtatg cttttgttgc cactgtttta acattgtcgg taactgtggc tcggtaagct
    10681 gcatagggct aagttcgtaa agcatactga ttgtattttc atacaggcaa ggaacttttt
    10741 gcgagtcatc cggctttaaa ggcaatttat cggtttgcca ttgataccag atatcaccgc
    10801 caatgcgtgt gccactcgtt aataacgtat tatcaatgtc aaaaaccaat aatatcttat
    10861 cggatggata gagagtttcc ttttcggtga taattttact gaccgcacta tattcgccag
    10921 taactatatt ttttacctgt gcgtaagata aagcaggcag agcagagagc atcatccaga
    10981 gaatgatttt aaatttatta ttcatttttt attccagatt agaaagtata agtcagaccc
    11041 cagcgccagc ggatttggcg attacttgat gttttatcca cacctttata atccgcaata
    11101 ctcatatagg gtatccacga tccaattttg taccctactt taaatgatgc ggtatagttt
    11161 ttatggccat cattccaggt taaataacca ttattgtaat agaccaggtt taactgatat
    11221 tggaagttgg ttttttgtgg tcgatagtcg atccacacat cgtaacggcg gttattacga
    11281 tcatcgttgc cataaccttt ggatttatcg gtggctttat ccatgtcata acgaaaccga
    11341 cccgaaactt tccattcttt gctgaagtca taacccgcag aaagaccata ttcgtaagtt
    11401 gaccaatatt gcgtggtatc aagccagaac atgggttcta accagaattt tttgtcatcc
    11461 agcggcttaa agcggtaaac aacagataat cctgttccgt tattgccaaa ttgtgagggg
    11521 gcccacttat ctttatcacc ttcatcggtg ttatagcgta attctcccat caaaccgata
    11581 ccattactaa aaaatgtacc aaattcaacg cggtccgcgt gggtgcgagt atcatcaaga
    11641 tactcatgac gataatcaat aaatccatgc ggctgttctg cacagactag tgtaggtgac
    11701 aggcaggata acattgttat tcctgcaagt gatattttaa gataagaatt cataatattc
    11761 cctttcagaa taaaatatat ttatggtggt cgaatacact atctgatgat tccaggtatt
    11821 agaatgtttt tgtatctata gcttgacttt aaattttaag tttgttttcc ttatcaatga
    11881 atagataaaa tttcgtctgt tgatggggtt ggaaattatt atcagataca aatattaacg
    11941 atgtatgccc atcaatcaga ggaccgaagg ttaccccttc aatacaatcc gcagaggaag
    12001 tgtaatcagc aaaatcaatt aataattttt tactgacggg ttgtaacgtg gatttattgg
    12061 accaatcttg cagactgtca atattcttga tatcgctggc agcagttaaa tcaaccatat
    12121 aaactctgac agagtaatcc cagtcattga acccagcact gacgtttctg ccggaacgtt
    12181 cgatgaccaa caattggtga tcattcagcg ccaggacctc ggatacgcca ttatcattaa
    12241 taccgaactt actcacttgc ggaacaggat cagtaatata gagatattca tgcaattgct
    12301 ttgaaggtcg accgtctgca tctatagaaa attgcagtaa gcgtgctgtg cctgaattta
    12361 ttggacttgc aataggccca tcctgtttaa ggctgctttc aacggcgata aaaagtgatt
    12421 ttccatctgg tgtaactgtc aggccctccc aggcgttgcc gctgcgaata ccattgctgc
    12481 tctctttgtc agaactaata ttaaagcgag caggaaataa agacgtaaaa tctttctcca
    12541 ttacgccatc gagagtgctc agacgcagtg gagcacctaa ctccgaactc cagagtaatg
    12601 atttaccatt gtgtgtcagg gccagacctt cggcatccac cgtattggtc acgaaaggct
    12661 gctgttttgc atcaagcagt ggacgctggt tgagaaaatt cacttgctca atgcccctgg
    12721 atgatacctg tatttttagt gtataaagac tggcatctcc t
//
LOCUS       DAPHJE010000120        12558 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPHJE010000120 DAPHJE010000000
VERSION     DAPHJE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.121"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            89..1624
                     /locus_tag="RH222_003618"
                     /pseudo
     CDS             89..1624
                     /locus_tag="RH222_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1670..1894)
                     /locus_tag="RH222_003619"
     CDS             complement(1670..1894)
                     /locus_tag="RH222_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HDV3798580.1"
                     /translation="MFESQNLTDKQIHNYAQQLAGDTPLKEVRPGIYTAKLDNGTSIT
                     LRNLSSSQEQTGARWTIDIKVRITVKEIGE"
     gene            complement(2068..2751)
                     /locus_tag="RH222_003620"
                     /pseudo
     CDS             complement(2068..2751)
                     /locus_tag="RH222_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(3269..4446)
                     /locus_tag="RH222_003621"
     CDS             complement(join(3269..4188,4188..4446))
                     /locus_tag="RH222_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3798581.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYLFYCKTVANIT
                     "
     gene            4551..4694
                     /locus_tag="RH222_003622"
                     /pseudo
     CDS             4551..4694
                     /locus_tag="RH222_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5191..5433)
                     /locus_tag="RH222_003623"
     CDS             complement(5191..5433)
                     /locus_tag="RH222_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798582.1"
                     /translation="MSGNSQHLSELFIIFIIAFLSIVVSFLCIDVFFSLVEWLITGNL
                     NISLKEIRHVLFIGCVIGSFIGIGCALAKYFRLKGF"
     gene            complement(5430..5765)
                     /locus_tag="RH222_003624"
                     /pseudo
     CDS             complement(5430..5765)
                     /locus_tag="RH222_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267355.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5784..6931)
                     /locus_tag="RH222_003625"
     CDS             complement(join(5784..6694,6694..6931))
                     /locus_tag="RH222_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3798583.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            7010..7471
                     /gene="bmaE"
                     /locus_tag="RH222_003626"
                     /gene_synonym="afaE-8"
     CDS             7010..7471
                     /gene="bmaE"
                     /locus_tag="RH222_003626"
                     /gene_synonym="afaE-8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M agglutinin-type afimbrial adhesin BmaE/AfaE-8"
                     /protein_id="HDV3798584.1"
                     /translation="MALTCHAVTVTATHTVESDAEFTIDWVDAGPTTTNAKDGEVWGH
                     LDMTQTRGTPTLGRLSNPQGETSPGPLKAPFRFTGPNGHTARAYLDSYGTAIHNYAGD
                     NLANGLKVGSGTGDDPFVVGTTSRLTAKIFGDQTLVPGVYRTTFELTTWTD"
     gene            complement(7806..8666)
                     /locus_tag="RH222_003627"
     CDS             complement(7806..8666)
                     /locus_tag="RH222_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV3798585.1"
                     /translation="MTTATSLNELQKQIRDRYDSLSKRLQQVSRYVLDNTNSVAFDTV
                     AVIAERADVPPSTLIRFANAFDFTGFNEMKQLFRMHLVEETASYSDRARLFREMESEA
                     VPETPLDILQEFASSNSQALQQLAARAEPEMLEHAVQLLAYADTIYIAGLRRSFSIAA
                     YLTYALSHLECRPILLDGMGGMLREQISRIKACDIVVSISFSPYAEETVMVSKTAASV
                     GARQIVITDSQISPLATFSDLCFVVKEAQVDAFRSQSATLCLVQSLVVALAYRLGDKK
                     HNNTQENSNQ"
     gene            8957..10462
                     /locus_tag="RH222_003628"
     CDS             8957..10462
                     /locus_tag="RH222_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3798586.1"
                     /translation="METVANFIHGECVTGSGQRIQAIFNPATGEQIRQVVMSTAQETE
                     QAIAAAQLAFPAWARFAPLKRARVMFRFKALLEENMERLARIISEEHGKVFSDAVGEL
                     TRGLEVVEFACGIPHLQKGEHSANVGTGVDSHSLMQPLGVCAGITPFNFPAMVPMWMF
                     PIALATGNTFVLKPSEKDPSLALALAKLLKEAGLPDGVFNVVQGDKESVDVLLTDPRV
                     QAVSFVGSTPIAEYMYLTASAHGKRCQALGGAKNHCILMPDADMDMATNAIMGAAYGA
                     AGERCMALSVVVAVGDKTADELCARLEKKIASLRVGPGLNQTPENEMGPLISAAHRSK
                     VLGYIDSGEAQGANLRVDGRGLRVKGHEQGYFVGPTLFDNVTPEMTIYKEEIFGPVLS
                     VVRVADYASAVDLINRHEYGNGSAIFTRDGGCARRFCEEVQAGMVGVNVPIPVPMAFH
                     CFGGWKRSIFGALNVHGNDGVRFYTRMKTITARWPEMQQETAAAFSMPTLG"
     gene            10474..12399
                     /gene="iolD"
                     /locus_tag="RH222_003629"
     CDS             10474..12399
                     /gene="iolD"
                     /locus_tag="RH222_003629"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDV3798587.1"
                     /translation="MQTERMTMAQALVRFLNQQYVSVDGKETPFVEGVATIFGHGNVL
                     GIGQALEQDPGHLQVMQGCNEQGIAHMATGFAKQHRRQRIFAVTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDIYACRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPE
                     QLMSAMLSAMRTLTDPANTGAVTICLPQDVQGEAWDYPLSFFARRVHHIERRPPDPQR
                     LAQAHALISRKRRPLVVCGGGVRYSGAHEALREFVEVLQLPFAETQAGKGALVSDHPL
                     NLGGVGVTGGMAANQLAPQADLVIGIGTRLTDFTTGSKALFSHPDVEFLLLNVAEFDS
                     LKLDATALIADARTGLTALTHALKDYRSGWGEAIAHAKSAWHDECCRLWERQWHPDDV
                     PEVAGHLDAQLMEYSETLNTHLTQTRVLGLINQHIEDNAILVGAAGSLPGDLQRLWQV
                     KTPDSYHLEYGYSCMGYEVAAAIGAKLAKPQQPVYAMVGDGSYMMLHSELQTAVQEGI
                     KVTILLFDNASFGCINNLQMGHGMGSFGTENRYRNPKTGQLDGPLVKVDFAQNAESYG
                     CRAWRVHNEKSLLAALEASRAHPGPTLLDIKVLPKTMTHDYASWWRTGNAQVAESPAV
                     RQAAEQTQAQVKKARQY"
BASE COUNT         2980 a         3263 c         3179 g         3136 t
ORIGIN      
        1 ttaatgcgta ttttcatgaa aggagatcac tcaataaccc cctgtgtcag catagttgtc
       61 gcctagattt ttacacagga gagagactat gcccgcacat gcagtaccaa ttgatataaa
      121 agaacagggc gttacctggt tattaccacc ttatagttgg tccatacggc atattgctcg
      181 taaactggac ctgagccctt cagtaatagc aagatggaga cttgaactgg tgcaacaagg
      241 cctgttacct gaaaatgaaa aaatatcttc tgtcgatgga aatgaatgga caccggagcg
      301 acgattttct gttgtaattg aaactgcaac attatctgaa atggaactgg ctgattattg
      361 tcggagaatg gggttatttg tcgagcaagt taaagaatgg agaactatat ccattcaggc
      421 tcatgaaatg aaaatggcag aaagtcgccg tgctgataaa gaattacgca aagcaaagac
      481 ccggttacgg gaactggaaa aagaacttct ccgtaaagat aaagctctgg cggaagcaac
      541 tgctcttttg atgttaaggg aaaagttcaa tgccctctgg gacaacagcg aggaaggctg
      601 actcctcttc cagagcggca gaagattatt aatctggtca gcgaggctgt cagttcagga
      661 gcgagaaaaa cacaagcctg ccagtgtatt ggtatttcag tcagaacact tccacgttgg
      721 atgcagggtg gtgtggttca tgcagatcag agaccaggtg catcaaggcg taccccgggg
      781 aataaactgt cagaaacaga aagattaaga atacttgatg tatgcaattc tccggagttt
      841 gcatcattgt ctccgaatat tattgttccg actcttgccg acagaggggt ttatatcgct
      901 tcagaatcaa ctttttatcg cgtattaaaa tcagctaacc aactgacaag gagaacccgt
      961 gaacaccaat ataagcggcc tgaagtactc aaagctgtag cccccaacca ggtgtggatc
     1021 tgggatatta gttatatccc atcatgggtt aaggggcagc atttttatct ctatatgatc
     1081 gtcgatattt atagccgaaa aattatagct gccgaagtat ttacgaaaga atctgggcag
     1141 tatgcggcag aactgcttca acgtgctgta tggaatgaaa aatgttcatc ccgcaatctc
     1201 gtgcttcatt ctgataacgg aggaccaatg cgcagttgta cgttactggc aaaaatgtac
     1261 gctcttggcg tattaagttc ttattcccgt cccctggtca gcaatgataa cccttattcc
     1321 gaatcgctct tccggacgat taagtattgt ccatggtggc cagagaatgg ttttcgaacc
     1381 atagatgaag ccagaagttg gttaagcagg ttcacgcgat ggtacaacct ggaacacaag
     1441 ccagcggtat taagtatgtt actccggatg aacgccatcg cggaattgat gcacaaatac
     1501 tggaagccag aaaaaagttt atcgcgaagc tcgcaaacgt catcctgagc gctggagcaa
     1561 acagttgcgt gactgggaac tcattcaggc agtttatctg aatccagaaa aagaagcggc
     1621 ataactgtaa ttttattaca tcaggcgaca actaccttga aaaacgccgt tactcaccga
     1681 tctccttcac ggtaatacgc accttaatat caatagtcca tctggcacct gtttgttctt
     1741 gagacgacga taaatttcgc aacgtgatcg atgttccgtt atccagcttt gcagtataga
     1801 tccccggccg aacctctttc agcggtgtat cgcctgccag ttgctgtgcg taattgtgga
     1861 tctgtttatc cgtcaaattc tggctttcaa acactttcgt cgtaccataa cggttactta
     1921 ccaaactctg cctgatagtc tgcccatcca ccgttaactc tccaaccggg tgacttttat
     1981 catatatttt actcagttca tcaaatcgtt gttgctgaac ctcctgatga cgggcttttt
     2041 cttcattatc tggtccccat cccccggtgt tccagcacct gcactaccaa gctcggcttt
     2101 ctcattgtcc gttgcgccag ccagttcttt accctggtta ggctgtttat cttcgtctgt
     2161 attgtcgcca ttcagcacat gccccggtcc ccgggtgcca tcgcgactcg gatcacgtac
     2221 agcacactgt tcccgtgagc cttcacaacc accaccggta tcccagctat tatcattccc
     2281 ggtatcctta ctggcattac ctgtagcacc agctattgct gctgccgtat tagctgctgt
     2341 ttcagtggct atcacgttca gcatattgtt ttcagccgag ttcctacctg cctgtgcacc
     2401 cgtaccggca gcacttgtac tgttgccggc aagcccgccc gcaatacctg aggcaatggt
     2461 gaccagcatg ctgatttgct gacgttcctg ttcactcagg ttatcggtac aaccaccgta
     2521 gtagttatca atgatatacc gggcagcaag ttcaccactg aatgcccctg cagcaccgga
     2581 agccgcattg tttccgccca gttgtgcagc aagcgctccc cagactgcgt gagccatcag
     2641 gtttgcttcc cggttgtcac cggtagcctg ttttataagc tgtgccactg ctggattcag
     2701 accgccagcc agtacctgcc ccggattccc tgcattcagt ccgcccagca cgcccgtgat
     2761 ggcctgtacc accatctgcg gcgtactgcc cgtaccgtag cctttcatga cctctctgta
     2821 ttccggggtc tcccgtaact gtgcatcact cagccctgga tatttcgccc tggctgcttt
     2881 catcgcttca gtttcgccat acgtcatgac gatgttactc atctgaccgc taatctggct
     2941 gataatctgc gcttcctgca gacgcttctg ctctttctcc ctgtcaaata tcggtgcaat
     3001 gctgccgttg gcgttttccg ggtcccggct gagcccggca atattctgct tctgactctc
     3061 cttatcacgg ataatgagat ttctgccgct gatggcggat gacgtggtac cttcagcatg
     3121 tccactgctg ccggacatac cggtaagtgc gttcgccgca ccggctattg catttgaggc
     3181 caccatctgg ctggctgaca tccctgagcc acccgataaa ctgattctgg tatggctggc
     3241 tttatagtct gcctggttat tgtagtggtc aagtaatatt ggccacggtt ttacagtaaa
     3301 aaaggtatct gttctctgac tcttccggcg tcagcccacc gttataatgg tgaggtctga
     3361 cgctgttgta gtagttcaat atgtaatcat taatttgctg ccgggcctcg tccttgcctg
     3421 cgtaaccatt cgttggcacc cattctgttt tcagactgcg gaagaagcgt tccatggggc
     3481 tgttatccca gcagtttccc cgccgactga cgctttgatt tattctgcaa cgccagagaa
     3541 gttgttgata tttaaggcct gtatactggc ttccctggtc gctatggaac acgacatccc
     3601 ggggttggcc acgcgtctca taggccatcc gcagggcact gcttatcagt gcggtatcgg
     3661 catgcgctga cagactccag ccgataaccc tgcgggcaaa aagatccata acaaccgcca
     3721 gatagcacca gcgatttcct gcccagagat acgtaatatc tccacaccat acccggtctg
     3781 gctccggtac tgcgaactga cgctcaagca gattcggcag gctggtatgc tcctgacggg
     3841 catttttata ctgatgtttt ccgggctgac aactgctcag gttcagatat ttcatcagac
     3901 gtccggcacg gtaacggctc atcgggacgc cgttttgagt cagcatttca gccagagtgc
     3961 gcgcgcccgc agagccccga ctttggttcc acgcccggcg tatttcgctg cacaacctga
     4021 ctcgtgccgg attaaccgta tcgcgtcgtt ttcgccagta ccggtaactg ctgcggtgta
     4081 tttccagagc agaacacagg ctgacaaccg tgtggctgtc acttagtctg gcggctatcg
     4141 tgaaccgttc agcgagtcgg acattaagag cgcggtagcc ttttttaata tcgtattttg
     4201 ttcctccaga cggcgaactt gcttttccag ctcgcggata cgttgctggt ctggagtaat
     4261 gggtgtggca gagggcgtaa taccctggcg ctctcgcctg agctggcgta cccagctctc
     4321 aagcgtggtt gaaccgacat tcatcgcttc actggcctgt cgatatgagt agcccttatc
     4381 aacaatcagc tgtgcacatt ccagcctgaa ctcaggggtg aaggttcgtt tggttttctt
     4441 gttcattaag tcacctgttt tgtgttgtgg tgagaatatc acctttcatc aggtggccaa
     4501 atttagtgtg ccactacata tggatatccg accagcccag cgtgttggta ctaatgagct
     4561 tgctggaaac cgcgaaacgc aacggtctgg agccgcatgc ctggctgaca gacgtcctga
     4621 tgcgtctgcc ggagtggccg gaggagcgac tggcagagtt gctgcctctt gagggattta
     4681 ccttctccgg gtgagtgata cctgccgtca ggtgttcgtg caccgggcca taacctgcag
     4741 tcgggagttg aactcctgac gacaggaaat gagccagacc agcaaccaca cgggctgaca
     4801 gcgaataaga acctgaatca gcccggagtc attcccgggt cagtacatca tcacctgcca
     4861 ttaatccccg agtcagtact tcgcggcctg acgcgatcac cctactcagt acttcagagc
     4921 ccgcctccgg tacctgcctg gcgatcgccc ctgtgcaaca aaataaatca accgttatca
     4981 gaccacgatc gttcgtatgc gccgccgatg acgcgcgcct gaaaaacgcg tcaatatgag
     5041 ttccccggaa gcttacccca ggtcaattgc cgtccatttt gcttctgtct gagccttaca
     5101 ggcagcagtc tgacatttag ccagctcatc ggctttttga gctttctggg ctttactcag
     5161 atagttattc tcaacaacaa ccacagggga ttagaagcct tttagcctga aatattttgc
     5221 gagagcacat ccaataccaa taaatgagcc aatcacacat ccgataaaca aaacatgccg
     5281 aatctctttc aaactaatat ttaaattacc tgttatcaac cactccacca aagaaaaaaa
     5341 cacatcaata cataggaatg acaccactat agaaagaaat gcgattataa aaataataaa
     5401 caattctgat aagtgctgag aattgccgct cattttttca cctccggaat gtaagactca
     5461 atctttttgc cttcatactc agaagcaaaa gaagccgaca catccccagc tataccagga
     5521 atcctactgg gtgtcatttc ttttgatagc cccaattctc ctttaatatc ggtatatttt
     5581 tgaagtgttg gattaaattt cgggtcccag ccgtctttta accagttagc accactatta
     5641 atgccccatg aaaggccttt accaatgcca tatccaatag cagaaccagc accattgatc
     5701 aacgcaccag atgttggggc ttttccttcg agccagtttc ctaatgctcc tccagttgca
     5761 ttccatgaat cgccacggat aatctagaca cttccgagcc gttgataata ctggttttca
     5821 tattctgtcg gtgacatctg ttcgctagaa ccatgccgac gcttactgtt ataaaacatt
     5881 tcgatgtaat caaaaatatc actgcgggct tcttcccgcg ttccgtagat ctttttcttt
     5941 atccgttcac gtttcaacaa ctggaaaaaa ctttctgcaa ccgcattatc atggcagtta
     6001 ccgcgacggc tcatgctacc ctccaggccg tgtgatttca ggaacgactg ccactcatgg
     6061 cttgtgtact gactgccctg atccgaatga accagcacct gtttttcggg attacgccgc
     6121 catacagcca tcagcagtgc gttcaggaca atgtcctttg tcatccggga ttgcatggac
     6181 cagccgataa ttttgcgtga gaacagatca acaacaacgg caagatacag ccagccttcg
     6241 tgggtcctga tgtaggttat gtccgttacc caacgctcat caggagcatc cggattgaac
     6301 tgtcgctgga gcctgttggg tgacacgata ctggcctcgc ctttacgtgc ccgcgggctt
     6361 cggtatccga cctgagcctt tattccgaca cgtttcatca gtctccagac tctgtttact
     6421 ccgcactgtt gcccgctgtc acgcaaatcc agatggattt tgcgataacc atagacgcat
     6481 cccgattcca gccagaactg tttaatctgt cctgtcagtc tcaggtctgc ctgatggcgt
     6541 tgtgaatgcg gctgctgaag ccaggcgtaa aaaccactgg gatgaacatc cagcacccga
     6601 cagagcaggc gaacaggcca gcaacaggag ttgtcacgga taaaggcgta cctcagtcgg
     6661 acagctttgc gaagtacgcc gcggcttttt ttaatatgtc ccgttcgtcg gtaacccgtt
     6721 tcagctcttt ctggagacgg cggatctcgg cctgagcatc tgactgttct ttattagtgg
     6781 aagaatccgg accgtacttc tttatccagg cataaaggct gtgggtggtg atatcgagac
     6841 gtgttgcaac gctggcaaca gaataaccgc gatcaacaac ctgtttgact gcttcagttt
     6901 taaactcttc gggataacgc ttaccgctca tgggcacctc tctttaagcc atcttaaatg
     6961 actctgaggt gtctgttaaa cccgtggcga ttcaattgca ggtataacaa tggcgctaac
     7021 ttgccatgct gtgacagtaa cagccactca tacagttgaa tcagatgctg aattcacaat
     7081 agattgggtc gacgctgggc caacgactac aaatgcaaaa gatggtgagg tttgggggca
     7141 ccttgatatg actcaaacca ggggaacacc aacattagga agactcagca atcctcaagg
     7201 agagacttcg ccaggaccgt tgaaggcgcc attcagattt accgggccaa atggtcatac
     7261 tgcaagagcg taccttgatt catacggcac agcgattcac aactacgcag gggataacct
     7321 tgctaatggg ctgaaggtag gtagtggaac cggagacgat ccatttgttg ttgggacaac
     7381 aagtcggcta actgcaaaaa tcttcggaga tcagacattg gttccaggag tctaccggac
     7441 aacctttgaa ttaactactt ggaccgactg acggaaaatt aacctgatga aagaaggggg
     7501 ctatatgtcc cccttgttga tttaccaaca taaaatggtt gtgtttaaat cagcctggca
     7561 gtgaaaagta gtcatgagat tataaatgtt gctgcccaaa agtttaaaga agccgttatc
     7621 tttgggttac attactacct acaagagaaa tcttcctcgc taataaggcc ctcgctgtac
     7681 gctaacatga ggcatgacct tttagggaga tagcagtaca gttgatattc aagctgaagt
     7741 ctgtatcgct actggcaccg aatcatttac tcttatatgg actcggggga ggtgttcatt
     7801 ggtcattatt ggttactgtt ttcctgggta ttattatgtt ttttatctcc taaacggtaa
     7861 gctagcgcga ccactaacga ctgcaccaga cacaatgttg ccgactgaga ccgaaatgcg
     7921 tccacctgag cctctttcac cacaaagcag agatcactga acgttgccag tgggctgatc
     7981 tggctatccg taatcacaat ctgccgagcg ccgacgctgg cggcggtttt gctgaccatc
     8041 accgtttctt ccgcgtacgg cgagaaactg atcgacacca caatgtcgca cgccttaata
     8101 cggctgatct gttcgcgcag catgccgccc atcccgtcaa gcagaatagg gcggcactcc
     8161 aagtggctta aagcataggt gagataagca gcaatgctaa aggatctgcg taatccggcg
     8221 atgtaaatgg tgtcggcgta ggccagtagt tgtacggcgt gctccagcat ctccggttcg
     8281 gcgcgggcag ccaactgctg cagcgcctgc gagttcgaac tggcgaactc ctgcaaaata
     8341 tcgagcggcg tttcaggtac ggcttcgctc tccatttcgc ggaacagtcg cgcacgatcg
     8401 ctgtagctgg cggtttcctc caccaggtgc atacgaaata actgtttcat ttcgttgaag
     8461 ccggtgaagt caaaggcatt ggcaaaacga atcagtgtag agggtggaac atcggcacgt
     8521 tcggctatga ccgcaaccgt atcaaacgcg acactgttcg tattatccag tacatagcgg
     8581 gagacctgct gcagcctttt gctcaggcta tcgtagcgat cgcggatttg cttttgtagt
     8641 tcgttcaggc tggttgctgt cgtcatgtta tccccctatc cagtctcatg attctaaacg
     8701 caaaaccgcg tcttttaaat gttggccatc cacctggcag caaaaatgaa acagaagctt
     8761 cagtcatttc ctcgcccgaa actcctaata gctcaggctg agtcagtgat gctggctgtt
     8821 cacataaaaa tccatccttg atcacaataa tcattattta ttttatttca acttaaaatg
     8881 gaatatttgt ttcatatata gtgttcagta cataacgttc aggtaacagt cacgtatcaa
     8941 cagcgagagg ttaaggatgg agacggtagc gaattttatt cacggcgaat gtgtcaccgg
     9001 ttcaggccag cgcattcagg cgatcttcaa cccggccact ggcgagcaga ttcgccaggt
     9061 ggtgatgagt acggcacagg aaaccgaaca ggcgattgcc gcagcacagt tggcgtttcc
     9121 cgcgtgggca cgttttgctc cactcaagcg cgcgcgtgtg atgttccgct tcaaagcgtt
     9181 gcttgaagag aatatggagc gtctggcccg tattatcagt gaagaacacg gtaaggtctt
     9241 ctcagatgca gtaggtgaac tgacccgtgg tctggaagtg gtagaattcg cctgcggcat
     9301 cccacatctg caaaaaggcg aacactctgc gaacgttggc acaggtgtcg acagccactc
     9361 actgatgcag ccgctcgggg tctgcgccgg gatcacgccg tttaacttcc cggcgatggt
     9421 gccaatgtgg atgttcccga tagctctggc gacaggcaac accttcgtgc tgaaaccgtc
     9481 ggaaaaagat ccgtctctgg cgctcgcgct ggcgaaactg ctgaaagagg caggcctgcc
     9541 ggacggcgta ttcaacgtgg tgcagggcga taaagaatcc gttgatgtgc tcctgaccga
     9601 cccgcgcgtg caggccgtca gttttgtcgg ttcaacaccg attgccgaat acatgtacct
     9661 gactgcgtcc gcccacggta aacgctgtca ggcgctcggc ggtgcgaaaa accactgcat
     9721 tttaatgccg gatgccgata tggatatggc gacaaacgcc ataatgggcg cagcttatgg
     9781 tgcggcagga gaacgctgca tggcgctttc cgtggtggtg gcggttggcg ataaaacggc
     9841 tgatgaactc tgcgcccggc tggaaaaaaa gatcgcttcg ctgcgcgtcg gacctggcct
     9901 gaaccagacg ccagaaaacg agatggggcc gctgatctcc gccgcacacc gcagcaaagt
     9961 gttgggctat atcgacagtg gcgaagctca gggggctaac ctgcgtgtgg acgggcgtgg
    10021 cctcagagtg aaagggcacg agcagggcta tttcgttggg ccgactctgt tcgataacgt
    10081 caccccggag atgaccatct ataaagaaga aattttcgga ccggtgcttt ctgtggtgcg
    10141 cgtagcagat tacgccagcg cggtggatct gattaaccga cacgaatatg gtaatggctc
    10201 ggccattttc acccgtgacg gtggctgcgc acgccgcttt tgtgaagagg tgcaggcggg
    10261 tatggtgggg gtcaacgtgc cgatcccggt gccaatggca ttccactgtt tcggtggctg
    10321 gaaacgttcc atttttggcg cgctcaatgt tcacgggaac gatggcgtgc gtttctacac
    10381 ccgaatgaaa accatcactg cacgctggcc ggaaatgcag caggagacag ctgcagcgtt
    10441 ctccatgccg acgctgggct gacagggggt tttatgcaaa ccgaacgtat gactatggcc
    10501 caggcgctgg tcagatttct taaccaacaa tatgttagcg ttgatggtaa agaaacgccc
    10561 tttgtggagg gcgtggcaac gatctttggg cacggtaacg tgctcggtat cggccaggcg
    10621 ctggagcagg atcctggtca tctgcaggtg atgcaaggct gtaacgagca ggggattgcc
    10681 catatggcga cgggattcgc caaacagcat cgccgtcagc gtatttttgc tgtcacctca
    10741 tccgttgggc ctggcgctgc caatatggtg acggcggcag cgacggccac ggcgaaccgc
    10801 attccactgc tgctactgcc aggggatatc tacgcctgcc gccagcctga ccccgtgttg
    10861 cagcagatcg agcagtatca cgatctcagc atcagtacca acgattgctt ccgcccggtt
    10921 tcccgctact gggaccgcat caaccgtcct gaacagctta tgagcgcgat gttaagtgcg
    10981 atgcgaacgc ttactgaccc cgcaaataca ggggcggtga ccatttgcct acctcaggat
    11041 gtgcaggggg aggcctggga ttaccccctg agtttctttg cgcgtcgggt gcatcatatt
    11101 gaacgccgtc cgcctgaccc gcagcgactg gcgcaggctc atgcattgat ttcccgcaag
    11161 cgccgtccac tggtggtgtg cggcggtggc gtgcgttact ccggtgcgca tgaggcgtta
    11221 cgggaatttg ttgaagtgct gcaattgccg tttgccgaaa cccaggccgg taagggggcg
    11281 ctggtaagtg accacccgct taatctgggc ggggtgggcg tgacgggcgg aatggctgcc
    11341 aaccagcttg cgccgcaggc tgacttagtg attggcatcg gcacgcgcct gacggatttt
    11401 actaccggct ctaaggcgct cttttctcat cccgacgtcg agttcctgct gctgaacgtg
    11461 gctgagtttg actccctgaa gctggatgcc accgcgctca ttgccgatgc ccgaactggt
    11521 ctgacagcgt tgacccacgc actgaaagat tatcgtagtg gctgggggga ggcgattgcc
    11581 catgcgaaat ccgcctggca cgatgaatgc tgtcgactgt gggaacgcca gtggcatcct
    11641 gacgacgttc ccgaagtggc agggcacctt gatgcacagc taatggaata cagtgaaacg
    11701 ctcaacacac acctgaccca gacgcgggtg ttggggctca ttaaccagca tattgaggat
    11761 aacgccattt tggtgggggc ggccggatcg ctgccagggg atttgcaacg cctctggcag
    11821 gttaaaacgc ctgacagtta ccatctggag tatggttact cctgcatggg gtatgaggtc
    11881 gcagcggcta ttggtgcgaa gctggcaaaa cctcagcagc cggtgtatgc catggtaggc
    11941 gatggttcgt acatgatgct gcactcagag ctgcaaactg ccgtacagga gggaattaag
    12001 gtcaccattc tgctgtttga taacgccagc ttcggctgca ttaataacct gcagatgggg
    12061 cacggaatgg ggagttttgg cacagaaaat cgttaccgta atccgaaaac aggtcagctt
    12121 gacgggccgt tggtgaaggt cgattttgcg caaaacgcgg aaagctacgg ttgccgcgca
    12181 tggcgggtac ataatgagaa aagcctgctg gcggcgctgg aagcctcgcg cgctcacccg
    12241 ggaccgacgc tcctcgacat caaagtgcta ccaaaaacca tgacccatga ttacgcgtca
    12301 tggtggcgaa ctggcaacgc acaggtggca gagtcgccag cagtgcgcca ggccgccgag
    12361 caaacacagg ctcaggtgaa aaaagctcgt cagtattgag ttttcctgtc gtaacgaaat
    12421 tttcatttca tttttctttt ttcctctcac ccatcccctc tcccaacggg agaggggatg
    12481 gtacagtttt tattccctcc cccccttgta atggagggac atatcttcct cggcgttgtg
    12541 agattgataa caaaattt
//
LOCUS       DAPHJE010000121        12471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPHJE010000121 DAPHJE010000000
VERSION     DAPHJE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.122"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(109..1194)
                     /gene="dmlA"
                     /locus_tag="RH222_003630"
     CDS             complement(109..1194)
                     /gene="dmlA"
                     /locus_tag="RH222_003630"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV3798588.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            1297..2220
                     /gene="dmlR"
                     /locus_tag="RH222_003631"
     CDS             1297..2220
                     /gene="dmlR"
                     /locus_tag="RH222_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV3798589.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2347..2985
                     /gene="leuE"
                     /locus_tag="RH222_003632"
     CDS             2347..2985
                     /gene="leuE"
                     /locus_tag="RH222_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV3798590.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            3158..3517
                     /gene="yeaR"
                     /locus_tag="RH222_003633"
     CDS             3158..3517
                     /gene="yeaR"
                     /locus_tag="RH222_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV3798591.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYFGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            3521..3703
                     /locus_tag="RH222_003634"
     CDS             3521..3703
                     /locus_tag="RH222_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV3798592.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            3851..4099
                     /locus_tag="RH222_003635"
     CDS             3851..4099
                     /locus_tag="RH222_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV3798593.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(4158..4232)
                     /gene="yoaJ"
                     /locus_tag="RH222_003636"
     CDS             complement(4158..4232)
                     /gene="yoaJ"
                     /locus_tag="RH222_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV3798594.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4235..4333)
                     /locus_tag="RH222_003637"
     CDS             complement(4235..4333)
                     /locus_tag="RH222_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV3798595.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(4366..5391)
                     /gene="dgcP"
                     /locus_tag="RH222_003638"
     CDS             complement(4366..5391)
                     /gene="dgcP"
                     /locus_tag="RH222_003638"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV3798596.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            5574..5828
                     /gene="yoaF"
                     /locus_tag="RH222_003639"
     CDS             5574..5828
                     /gene="yoaF"
                     /locus_tag="RH222_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV3798597.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(5850..6197)
                     /locus_tag="RH222_003640"
     CDS             complement(5850..6197)
                     /locus_tag="RH222_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV3798598.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6252..7433)
                     /gene="nimT"
                     /locus_tag="RH222_003641"
     CDS             complement(6252..7433)
                     /gene="nimT"
                     /locus_tag="RH222_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV3798599.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     FNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            7530..8351
                     /gene="nimR"
                     /locus_tag="RH222_003642"
     CDS             7530..8351
                     /gene="nimR"
                     /locus_tag="RH222_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV3798600.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTHRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(8308..8754)
                     /locus_tag="RH222_003643"
     CDS             complement(8308..8754)
                     /locus_tag="RH222_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV3798601.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            8924..9028
                     /locus_tag="RH222_003644"
     CDS             8924..9028
                     /locus_tag="RH222_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798602.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(9029..9532)
                     /gene="yeaK"
                     /locus_tag="RH222_003645"
     CDS             complement(9029..9532)
                     /gene="yeaK"
                     /locus_tag="RH222_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV3798603.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(9575..11065)
                     /locus_tag="RH222_003646"
     CDS             complement(9575..11065)
                     /locus_tag="RH222_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3798604.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(11246..>12471)
                     /locus_tag="RH222_003647"
     CDS             complement(11246..>12471)
                     /locus_tag="RH222_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3798605.1"
                     /translation="IPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAF
                     LSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNI
                     LDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKI
                     LVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIE
                     VSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCV
                     MLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNS
                     AQAKIIAERMRKNVELLTGFSNRYDVPEQLTISIGTVFSTGDTRNISLVMTEADKALR
                     EAKSEGGNKVIIHHI"
BASE COUNT         3370 a         2926 c         3032 g         3143 t
ORIGIN      
        1 aaaacacgaa acggaaacaa taaaaggtga aatgacgcag gttatttact gcaaagaacg
       61 aataaaaaaa ccgcgtcagt gacgcggttc aaataaactg gtttgacctt aacgcaaaat
      121 aattttgcaa atcgcgtcgg caacctgtgg cgtggtggca ttgcctttca tatcaggtgt
      181 tttcggcccg tgagcaatca cttcttcaat tgctgccaga ataccgttat gcgcttgctg
      241 gaaacgctca tcgccattgc cgagaaaatc gagcatcatt gcaccggccc aaatcgtggc
      301 gatagggtta gcaatgtttt tcccgtagat atccggcgcg gaaccgtgga ctggctcgaa
      361 gagcgacggg aaagtgcgtt ccggattcag gttggcggat ggggcaatgc caatggtgcc
      421 ggtgcaggcc gggccaagat cggaaaggat atcgccaaat aaattggacg ccaccaccac
      481 atcgaagcgt tccggctgca tcacaaaacg cgcgcagaga atatcaatat gctgcttgtc
      541 ccagcggatc tccgggtaat tctcggccat tgcttccact cgctcatccc agtacggcat
      601 gctgatggct aaaccgtttg atttagtggc agaagttagc gtcttacgtg ggcggctttg
      661 cgcaagttcg aaggcataac gcaaaatgcg atcgacacca cgacgcgtaa ataccgattc
      721 ctgaatgacg acttcatgct ctgtaccttc attcactcta ccgccgagcg aggaatattc
      781 gccttcggtg ttttccctga ccacgtaaaa atcgatgtcg ccaggctgtt ttcccgccag
      841 cgggcaggga acgccaggaa agagacgaac cgggcgcagg ttgacgtact ggtcgaattc
      901 acgacgaaat ttcagcagcg aaccccacaa cgaaatatgg tcaggaacgg tgtccggcca
      961 gccgacggca ccaaaataga tggcgtcgaa acggctaagt tgctcatgcc agtcgtccgg
     1021 catcatttta ccgtgatggc tgtaatactc gcagctcgcc cactccattt gctcaaaact
     1081 caaggcgaag ccccagcgct cagcggcagc ctgtaacacg cgaatccctt caggaaggac
     1141 ttctttgcca atcccgtctc ccgggatcgc agcaatacgc atcgttttca tcattgggtt
     1201 ctcacttcct gaccatcaaa attgacaaca tcaccatcct ataattgact gaaaaggaat
     1261 taatccccgt aactggtgaa acataaaaca cgaatcatga ataatttacc gctgctgaat
     1321 gatttgcgcg tctttatgct ggtggctcgt cgggctggtt ttgccgccgt ggcggaagaa
     1381 ctgggcgttt caccggcgtt cgtcagcaag cgcatcgcct tgctggagca aacgctaaac
     1441 gtggtgttgt tacaccgcac cacccgccgc gtcaccatta ccgaagaagg agagcggatt
     1501 tatgaatggg cgcagcggat tttgcaggat gtcggtcaga tgatggacga actttctgac
     1561 gtgcgtcagg tgccgcaggg gatgctgcgt attatcagca gctttggttt tggacgacag
     1621 gtggtggctc cggcgttatc agcgctggct aaggcgtatc cgcaactgga attacgtttt
     1681 gatgtggaag accggttggt cgatttggtc aatgaaggtg ttgatctcga tattcgcatt
     1741 ggcgatgaca tcgcccctaa tttaatcgcc cgcaaactgg cgactaatta ccgcattctc
     1801 tgcgcgtcgc cggaatttat tgcgcaacat ggcgcgccga aacatttaac cgatctttcc
     1861 gcattgccgt gcctggtcat taaagagcgc gatcatcctt ttggtgtctg gcaattacgc
     1921 aataaagaag gccaccatgc gattaaagtc accggcccac tgtcgtctaa tcatggagaa
     1981 attgtccatc agtggtgtct cgatgggcag gggattgctc tgcgctcctg gtgggatgtt
     2041 agcgaaaaca ttgccagtgg tcatttagtg caagtgctac cggaatatta ccagccagcg
     2101 aacgtctggg ccgtttatgt ttcaaggctg gcgacgtcag cgaaagtgcg gataacggta
     2161 gaatttttac gccagtattt tgccgagcac tacccgaatt tttcactgga gcatgcctga
     2221 ttaatgattc aattatcggg ttgatatcag gttaaaacct gattttctcc tttctaagcc
     2281 gctacagatt ggttagcata ttcaccttta atcgcgcatg atcgaaagat aattaaagag
     2341 gttaatgtgt tcgctgaata cggggttctg aattactgga cctatctggt tggggccatt
     2401 tttattgtgt tggtgccagg gccaaatact ctgtttgtac tcaaaaatag cgtcagtagc
     2461 ggtatgaaag gcggttatct tgcggcctgc ggtgtattta ttggcgatgc ggtattgatg
     2521 tttctggcat gggctggagt ggcgacatta attaagacca ccccgatatt attcaacatt
     2581 gtacgttatc ttggtgcgtt ttatttgctc tatctgggga gtaaaattct ttacgcgacc
     2641 ctgaagggta aaaatagcga ggccaaatcc gatgagcccc aatacggtgc tatttttaaa
     2701 cgcgcgttaa ttttgagcct gactaatccg aaagccattt tgttctatgt gtcgtttttc
     2761 gtacagttta tcgatgttaa tgccccacat acgggaattt cattctttat tctggcgacg
     2821 acgctggaac tggtgagttt ctgctatttg agcttcctga ttatttctgg tgcttttgtc
     2881 acgcagtaca tacgtaccaa aaagaaactg gctaaagtgg gaaactcact gattggtttg
     2941 atgttcgtgg gtttcgctgc ccgactggcg acgctgcaat cctgatacat tcagcccgcg
     3001 ttgtcgcggg cttcccactt gtaatcctcc ctgatttttc tctgatatgg tgctaaaaag
     3061 taaccaataa atggtattta aaatgcaaat tatcaggcgt accctgaaac ggctggaata
     3121 aaccgttttc agcgcattca ccgaaggagg gaaaaggatg ctgcaaatcc cacagaatta
     3181 tattcatacg cgctcaacgc ctttctggaa taaacaaact gcgcctgccg gaatattcga
     3241 acgtcatctt gataaaggaa cgcgcccggg ggtttaccca cgcctttccg ttatgcatgg
     3301 ggcggtcaaa tatttcggct accctgatga acacagtgca gagcctgatc aggtgatcct
     3361 tatcgaagcg gggcagtttg cggtgttccc tccagaaaag tggcacaaca ttgaagccat
     3421 gactgacgat acttatttca acattgactt cttcgtggct cctgaagtcc tgatggaagg
     3481 tgcgcaacaa cggaaagtca ttcataacgg gaaatgaatc atgggcaaag caacgtatac
     3541 cgtgaccgtc accaataaca gcaatggcgt ttctgtcgat tatgaaacag agacgccgat
     3601 gactttgctg gtgccagaag tggcggctga agtgataaaa gatctggtga ataccgtacg
     3661 ttcttatgac acggaaaacg aacatgatgt ttgtggttgg taattatcac tgatcgttga
     3721 tggcgcaatt tctgcgccat cgcaaaaaaa acctccgtga tgctcgtcac aaatcttgcc
     3781 ctcccgatgt tgtgctatgc tttttatcag cgactaaccc gctaatgcaa aacagataag
     3841 tgaggaaaca atgggcattc tgtcatggat tatttttggg cttattgccg gtattctggc
     3901 gaagtggatc atgccaggta aagatggcgg tggattcttt atgactatcc tgctggggat
     3961 agtcggtgcc gtagtcggcg gatggatcag cacgctgttt ggctttggta aagtcgatgg
     4021 cttcaatttt ggcagcttcg tggttgccgt tattggtgcg attgtcgtgc tatttatcta
     4081 caggaagatt aaaagttaac gcttaaattg cacaaaggct gcacacaggc agcctttgct
     4141 attttttaga ggtgacgtta ccaccatccc agctcagtgc cgagtacgac aataatcgcc
     4201 acacccatca taataatcgt tgtttttttc atctttatgc tcccggggca gcatagccgc
     4261 caataaaaaa ccatgctaaa aatacgaccg ctgtaatgaa gattacaacc ggaaaaataa
     4321 taccgattcg catgttatca ccaatcaaaa ggatgtgaat acgcctcagg aatgtagcgc
     4381 tggatgcgcg acaaaaggtg ttttctgttt gtgttttttc tcctgataca tcgcaatatc
     4441 ggcagcatgc agggcactgt ctgcatcggt tgtttcagga tcgacttcta ctatgccaag
     4501 actggcaccg ggataataca aatcaacatc acctaagtga tattctccac gtatttgctg
     4561 ctgaatgcgt tctcgcagcg acgaaatatc cgcattctcg ttgtttagtg aaacaactaa
     4621 aaactcatca ccgccgagac gaccaataac ttcgccattt tgctggagcg tattaaggcg
     4681 ctcgccaacc tgaatgagaa acagatcgcc actattatga ccaaaacgat cattgattaa
     4741 tttgaagtta tccagatcga taaacgcgat cattatctta tggttaagat gccgggcgag
     4801 ggaaaacagt gtcgtcagat tttcaaaaat cgcccgccga ttcggtagcc cggttaacga
     4861 gtcggtatac gattgcgcaa tcagcgcagc attggcttcg cgcagctgtt caaccagtgc
     4921 ctctttttga atatattgtg caatcagtcc ggcgaataac tgtaaaacct gttccgcgcg
     4981 ttcactccac tggcgcttct cactgctggc ggcgcaaagc gtgccataga atgatccatc
     5041 cggtaagtga attggcgtgc tcagaaatgt ggtgatgcca agattgcgtg ccgcaataca
     5101 gtcaccccaa cggtcgggga cttcatcgct gaaaaagcag ttttcatcaa tggcgcgttt
     5161 gcataacgag taatcccacg agacggtaaa attctccggg atgtgcattt tctggctgtt
     5221 acgggcaaac ataatatgct gcaggcgcgc ttcgacatcc actttggtca ggtaggttga
     5281 ttccatatca gtgaccattt ccagcatctc cagaagctgt cggaccagac tttccagtga
     5341 ctgttctttg gcaagggatt gcgagacgcg ggcgataatc tgatctgaca cggaacaact
     5401 ccaggatacg gcgggtaaat gctgattatt taatctttgc aggcatgata aaacatcatc
     5461 aacaaagatg aaacaagacg taatatgttt tgagtgaatg taataaaaat agtgctgcaa
     5521 aacaaaacga taccgcgata ctctaatctt ggataaatat gtgagatcaa taaatgaaaa
     5581 ttatcagttt tgttctgcct tgcttgctgg tcctggctgg ttgttcaacc ccttctcagc
     5641 cagaagcacc taaaccgccg cagattggta tggcaaatcc ggcgtcggtc tattgccagc
     5701 agaagggcgg gacgctcatt ccagtgcaga cagcgcaagg ggtcagcaac aattgcaaat
     5761 taccgggcgg tgaaacgatt gatgaatggg cattgtggcg gcgtgaccat ccggctggtg
     5821 aaaaataacc gccggatgct gtactaaaat cacaagctac gtagccagtc ggccagcacc
     5881 agcgcatggt tctgcgtggt gttttttgct gcgtagagca gggtcagcgg ctgttttttg
     5941 gcgatgtccg ccagccgctt tccttcttgc tcgtgttgtg ccagttctgc aagatattgc
     6001 tcgcgaaagg ttgcaaaatc gacgacttcg ccgtgaaagg ctttgcgcag ttccgttgac
     6061 ggcgtgattt ctttatccca ctcatcaagg gctaaatcgg tttttttgat accgcgcggc
     6121 cagaggcggt cgaccagtat gcgataacca tcgctctgtt ctgccggatc ataaacgcgt
     6181 ttgcactgaa tattcatcgt gtttgttctc cctttcacgc atgatttgtc acgtcactat
     6241 agcaccggat attagcgaat ttctttgtct cttccggcgc aaagtccgaa aatcgccatc
     6301 agtagtgaaa gtattgcaac acccattagt ggtacggacc agtcaccgtt agcatcgtgt
     6361 attttaccca tcagcggcgg cccacaggct gccagcaaat acccgacgga ttgtgccatc
     6421 cccgagagtg ccgccgcctg atgcgcagaa cttgcccgaa gaccaatgaa cgtcaacccc
     6481 agtatcattg ttgcgccgga accaaaaccg aaaagcagag tccaggtgat cgcgtgtgct
     6541 ggcataaagc agagcccaac cgcgcccact gcgcacatca aggcaacgaa cgctgcaata
     6601 ccacgctgat ctttcacatg atgtaagaaa agtgggatca gcaaaccggg agctgctgtg
     6661 gctagttgca gcaaaccatg cagtgaaccc gcctgtgctt cgctatagcc gtgactgatg
     6721 aggatcgccg gaagccagcc aataatcacg taatagacca gtgagttgat cccaagaaac
     6781 aatgtgacct gccaggcaag cggcgaacgc cagatacccc gagtatgtaa ggcgcgcgag
     6841 gtactcaaat ttgcatgttg ttggcttcgc cactgtggca gccataaaaa aagagccagc
     6901 agaggaaaac acatcagcat gagcaacgcg ccttgccagc caaaaccgtt aaaagccagc
     6961 ggcactacca tagccgatcc aagtgccgcc gcagctccca ttgtcaggga atatgcgccg
     7021 gtaagtctgg cgacggaatg agggaaatcg cgtttaatta atcctggcag taagacatta
     7081 cctaatgcaa tcccaccacc aatgaccgct gtaccgccaa ataataagta aggcgaaggg
     7141 agagagcgaa ttgcgatacc agcacagatc aaaagtaacg cggcaaacag gctacgttcc
     7201 ataccaaaac gtcgcgctac cggggcggcc aaaggtgaga ttagcgcaaa ggccaataat
     7261 ggcagggtgg tcaataagcc ggtttgcgct gtcgtcagcg agtaagcgga acgaatcgta
     7321 tccagtaacg gtgctgcgcc ggtaaaggtg acgcgtaatg ttgtggcaat catcagaatg
     7381 ccagcgataa ggacaatcct gtttttgccg cttaatgaag ttgaacaggt catatgcttc
     7441 tcattgttac aaacgaggct gtaccttacc gtttttattg ttatgttgaa tcaagctaaa
     7501 atgacaaatt atcgctaaat tcggacaaaa tgatgcacag gttgaatctc aatggctatg
     7561 aacccgaccg acaccatgaa gccgcggtcg cattttgtat tcacgccgga acggatgagt
     7621 taaccagtcc tgtacatcag caccgcaaag gacagctgat actggcgcta catggtgcaa
     7681 ttacctgtac ggtggaaaat gctttgtgga tggtgccgcc gcaatatgcg gtttggatac
     7741 cgggcggggt ggaacatagc aatcaagtga cagctaacgc tgaactctgt tttctgttta
     7801 tcgaacccag cgcagtgaca atgccgacaa catgttgcac attgaaaata tcgcccttat
     7861 gccgggaatt gattttaacc ctggcaaaca ggacgacgac tcagcgtgct gagccgatga
     7921 cccacagatt aattcaggtc ctgtttgatg aactccctca gcagccacaa caacagctgc
     7981 acctgcccgt ttcttctcat cctaaaatcc gcacgatggt ggagatgatg gcgaaagggc
     8041 ccgtcgagtg ggggtcatta gggcaatggg ctggcttttt tgcgatgagt gaacgcaatc
     8101 ttgcgcgact gatagtaaaa gagacggggt tgagttttcg ccagtggcgt cagcagttac
     8161 agctgattat ggcacttcag ggattagtga aaggcgatac tgtacagaaa gttgctcata
     8221 cgctggggta tgattcaaca acggccttta ttactatgtt taagaaaggc ctggggcaaa
     8281 cgccaggccg atatattgcg ggattaacta ctgtttcccc acaatcagcg aaaccagacc
     8341 cgcggcaata agcgggccga ccggtacgcc gcgaaacagc gccacgccta aaacagtgcc
     8401 taccagcagc ccggcgacca gttgcggctg gctgcccatt aaggtcacgc cgcgcccacc
     8461 cagccaggag acaatcacgc ctactgcaat tgccaccagc gatttccagt tcagaaacga
     8521 gtgaatcaag gttgagggcg gtagcgtccc gctggcgatg ggggccataa cgccaatcgt
     8581 cagaatgatt atcccgatac ttagcccctg tttttcaatc cacgggaaaa aggtgcttaa
     8641 cggtgtgacg cggacaatga tcaacaccag aattgaaacg gcgacagtgg tgttatgact
     8701 gataaagccc agcgcagcta acccgagcag gatcagcaaa gtgacatcaa acatcgtggt
     8761 ttccttgcca aaaaataata agcctgctta ctttacgctt gctgcaggaa ggaagtagaa
     8821 aatctttacg ttttttgcat aaaaaatcag gcgaactgtc atcaaactgc catttgtgca
     8881 cgctaacaat aacgtgaaaa cagctatctc ccgggggcat gctatgaacg atcaaatgtt
     8941 cgtcgagaca ctgattatca cgtcatcgtt ttttgccatt gctgttgtgc tggttttgtc
     9001 cgttctgctt attgaacgga caggctagtt acgcagtgcg gcggaaattg agcagttctg
     9061 gttgcgcaat gcgcaaataa tccgtggttt tcaaaataac ggatttatcc agcatccctg
     9121 cattgaaggc gatttcatca aatcgttcga agagtagagg gtcggcaacc agtttgagtt
     9181 ttggatggaa gctgaaaggg gggatcgcgc cgaagacaca gcctgtgagt tcatcgactt
     9241 ccgccgggct ggccaacgaa gcgcgtaatc cgcctatatg gctggcgagt tggctcagat
     9301 cggcctgttg atcggcagcg aggatcgcca gaacatgttg atttacgcca ttgcctttga
     9361 ctttacatac cagtgcctta gctccctggc ctaaagcggt gccacgaatt tctgacaccg
     9421 cttcacattt acctaccgct tcatgggtaa cgacgcggaa gttagcacct tcttgtgata
     9481 ataaagcgat taaccgctga tgcgtcacgc ttcctttagc catttcagtc atttcacgct
     9541 cctgagatta caagcaaaca accacagaag gttatcatga acggctgttt ttgttctgct
     9601 tattgacata taaacgctca tcggaagctt tatacgcatc atgtaacgta tcgttttctt
     9661 tcatcgcgta aataccggaa gagaagccga tctctttctg cggcgcgata tgttgcagac
     9721 gtttttcgat acgttcaggc agttgtgctg caatttgcgg cgtcgaatcg acaagaatga
     9781 tgcagaattc atcgccaccg agtcggatgg cataatcact tttacgaatc gattgtttaa
     9841 tcgcctgagc taacaacgta atcgctaaat ccccttcctg atgaccgagg gtgtcattta
     9901 ttagctttaa cttgtccatg tcaattgcaa taaacatcac cgaagaaccg gattgcacca
     9961 gtttctgcaa ccgctgctcc agttcagggg ttaaaatttt gcgattatac agtccagtca
    10021 tcgcatcact aatattttct cgcgacacat tttggtataa gcggaagtgc atccgcacca
    10081 tattcagcaa aattaacgcc gtcaaaatcc agaacagaac gcttttccac gaagacgtga
    10141 taaagtaaag aatatcaatg gataacgaga cacgaatgcc gcccggtaag tcatggacgt
    10201 aactgacata ctggaacaga ttatcttcgc tctggttgat gataatgtcg cgccccgaat
    10261 cggtatcggt tagcgtgaca ttgagaaaac gccagaggag agggcggtca tgagtgtaaa
    10321 agatattccg caaattgttt ttgttaatat ccagcagcac aatccctttt agctgccctg
    10381 caacatacac cggcgtcagc aaactcatca cgttctgttt agtacgctga tcctggtaaa
    10441 tgctcgacaa tacggtacgc ccggtaaata ccttttcaat atctttgcga tcgataccaa
    10501 tacttcctga ctgcaggaag gaccagttat tcatcgcaaa gtattcaaca ttaaccggct
    10561 cataaaaata gacatagcgg tcattgagat cgagataata gtgaaatttc tcttcggcca
    10621 tcccaagacc tgaaccgtaa tcatcctggt ggcggttgcc ttccactgcc tgatcaaaag
    10681 cgggtaatag tgctgcagct tccacaattg tgtcgcagtc agtggatgcg gtttgtagtg
    10741 tgccgcgtaa gcccgggtat ttatgggcag tcaggtttaa gccatgggta ttattggcag
    10801 actcaaaagc ctcgcagatg gcgcgccgct gttctggcga cacttccgac tgctcagcag
    10861 cgagagcgcg catcacatgc gcggcaatac tctgattctg atacttatca taaagaaagg
    10921 aggagtctgc tttctgcacg atatagcgca gataggcact cattgctctg tcgctggcga
    10981 caagttcaaa aataaggaag gaagatgtca gcacaatgac acttgcggcg ataaaatgcc
    11041 ggagcattct atggtgcaat ttcatgataa atggcactcc ttcggttagt gaaacgagaa
    11101 aaccagataa agttcgattg cggcctggca caggggcgct aattatactc atctctgcgc
    11161 cgctggggag gggaaattta ggggtaatag taataaattg tatgcaaaag ccacgcaaaa
    11221 agcatggctc tgaatatttt tgcacttaaa tatgatgaat aatcactttg ttgcccccct
    11281 cgcttttcgc ttcgcgtaag gctttatctg cttccgtcat gacaagcgag atattacgcg
    11341 tgtcacccgt tgaaaaaacc gtgccaatac tgatggtcaa ttgttcagga acatcatatc
    11401 tgttactaaa gccggtcagg agttcgacat ttttacgcat acgttccgca atgatttttg
    11461 cctgggcact attgagttcg gtaaatagca agccaaacac ctcaccctcc agtcgcgcta
    11521 aaatatcatc ggggcggatg ctttgctgga taatatcgac aattgaaacc agcactttat
    11581 cacccacacg atgtccccat tgggcattga tgcgtttgaa ctgattaata tcaaccagca
    11641 tgacacagaa gtccttaaca acaggtcgcg acaataatga ctctacgctg ttgaaaaagt
    11701 agcgccgatt ataaatatta gtcagcacat catgaactgc cagtttgctg gagagttgca
    11761 gttcctgaaa aatgttataa atcagaaaag acacaacaaa cagtttactg gaaacttcaa
    11821 tcccgcgact gatataccag atggtgtaat tatactcatc cagagtcagt agaagtaagt
    11881 tgcagcatag cgatgccaga cataataatg ctattaacgg ccatagttcg ctggctaatc
    11941 gtgtacgcat gataataaag aacagtaaaa atgcccacag acaaaccaat atttttgtat
    12001 agttgattcc ccaggtcgca gtatggttgt caggacagta gtcgacgaca tacaaagaat
    12061 agtcagcgtt ataactgctc agattgtgtg ccagaagggg aaaaactaaa aaaggaatca
    12121 gcgccagcaa caggattccc gtttttttct tattgttgtc aaggatgttg tcttttccat
    12181 aacaaaagag tgccagcgag gttaaacaaa taaaactgag ttggcggaaa atatagaaaa
    12241 tagaaacatc attcgacttt gtctggatca gtgtactgcc gttaattggt ttatggataa
    12301 ttacaatggt ttcaactaaa tagataaggc cacttaaaaa tgcaaggctt aaaatgagtg
    12361 ttggtaaaat ttgtttgtta gtgaaatatt tcattaacat gaaaagaaaa atgacggcgt
    12421 taaggaaaaa caaagccaca acaaccatca ggtaattcat ctgtgggatg a
//
LOCUS       DAPHJE010000122        12378 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPHJE010000122 DAPHJE010000000
VERSION     DAPHJE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.123"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            94..1257
                     /gene="emrK"
                     /locus_tag="RH222_003648"
     CDS             94..1257
                     /gene="emrK"
                     /locus_tag="RH222_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV3798606.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF"
     gene            1257..2795
                     /gene="emrY"
                     /locus_tag="RH222_003649"
     CDS             1257..2795
                     /gene="emrY"
                     /locus_tag="RH222_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV3798607.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(2903..4231)
                     /gene="dsdA"
                     /locus_tag="RH222_003650"
     CDS             complement(2903..4231)
                     /gene="dsdA"
                     /locus_tag="RH222_003650"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV3798608.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4249..4467)
                     /locus_tag="RH222_003651"
                     /pseudo
     CDS             complement(4249..4467)
                     /locus_tag="RH222_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            4647..5705
                     /locus_tag="RH222_003652"
     CDS             4647..5705
                     /locus_tag="RH222_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3798609.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            complement(5713..7146)
                     /gene="cscA"
                     /locus_tag="RH222_003653"
     CDS             complement(5713..7146)
                     /gene="cscA"
                     /locus_tag="RH222_003653"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV3798610.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            7362..8276
                     /locus_tag="RH222_003654"
     CDS             7362..8276
                     /locus_tag="RH222_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV3798611.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            8348..9595
                     /locus_tag="RH222_003655"
     CDS             8348..9595
                     /locus_tag="RH222_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDV3798612.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(10037..10111)
                     /locus_tag="RH222_003656"
     tRNA            complement(10037..10111)
                     /locus_tag="RH222_003656"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10076..10078),aa:Arg,seq:cct)
     gene            complement(10187..11119)
                     /locus_tag="RH222_003657"
     CDS             complement(10187..11119)
                     /locus_tag="RH222_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV3798613.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            11413..12168
                     /gene="mlaA"
                     /locus_tag="RH222_003658"
     CDS             11413..12168
                     /gene="mlaA"
                     /locus_tag="RH222_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV3798614.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         3101 a         2803 c         2997 g         3477 t
ORIGIN      
        1 taatgtgcaa cacatgcact gtgtttgata tgaagaatga atgctctttt cattcaattc
       61 ataaatttca tctatgagaa atgagagata atagtggaac agattaattc aaataaaaaa
      121 cattctaaca gaagaaaata cttttcttta ttggcggtag ttttatttat tgcgttttca
      181 ggtgcctatg cctattggtc aatggaatta gaagacatga ttagtacaga tgacgcctat
      241 gtcacgggga atgcagatcc aatttctgca caagtctcag gtagtgtcac tgtcgttaat
      301 cataaagata cgaactacgt tcgacaaggt gacattttag tttcactgga taaaactgat
      361 gccactatcg cactcaataa agctaaaaat aatctggcaa atattgttcg gcaaacgaat
      421 aaactatact tacaggataa acaatacagt gccgaagtcg cttcagcacg tattcagtat
      481 caacaatctt tagaagatta taaccgtcga gtgccgttag cgaagcaggg ggttatttca
      541 aaagaaacgc tggagcatac caaagatacg ttaataagta gcaaagcggc attgaatgcc
      601 gctatccagg cttataaagc gaataaagct ttagtaatga acacaccatt aaaccgtcag
      661 ccacaagtcg ttgaagcggc ggatgcaact aaagaagcct ggttggcgct taaacgtacg
      721 gatattaaga gtccggttac cggctatatt gcccagcgaa gtgttcaggt cggcgaaaca
      781 gtgagccccg gacaatcgtt aatggctgtc gtaccggcac gtcaaatgtg ggttaatgcc
      841 aactttaaag aaacacaact cacggatgta cggattggtc aatcggtcaa tattatcagc
      901 gatctttatg gtgaaaatgt tgtgtttcat ggtcgggtga cagggatcaa tatgggaacc
      961 ggcaatgcgt tctccttatt acctgcacaa aatgcgacag ggaactggat caaaatcgtt
     1021 cagcgtgtac cggttgaagt ttctcttgat ccaaaagaac tcatggaaca tcccttgcgt
     1081 attggtttat cgatgacagc aactattgat acgaagaacg aagacattgc cgagatgcct
     1141 gagctggctt caaccgtgac ctccatgccg gcttatacca gtaaggcttt agttatcgat
     1201 accagtccga tagaaaaaga aattagcaac attatttcgc ataatggaca attttaatgg
     1261 caatcactaa atcaactccg gcaccattaa ccggtgggac gttatggtgc gtcactattg
     1321 cattgtcatt agcgacattt atgcaaatgt tggattccac tatttctaac gtcgcaatac
     1381 cgacaatatc tggctttctg ggagcatcaa cagacgaagg cacctgggtt atcacctcgt
     1441 ttggtgtagc aaatgccatt gcgatccctg ttactggcag gttggcacaa agaataggcg
     1501 aattaagatt atttttactt tcagtaacct ttttttcgct gtcttcatta atgtgtagcc
     1561 tatcgaccaa tcttgatgtg ctgatatttt ttagagtcgt tcaggggtta atggcggggc
     1621 cgttaattcc actgtcacag agtttattat taaggaatta tccgccagaa aaaagaacat
     1681 ttgctctggc attatggtca atgaccgtga ttatcgctcc gatatgtggg ccgatattgg
     1741 gcggttatat ttgtgataac tttagctggg gttggatatt tttaatcaat gtccctatgg
     1801 ggattatcgt cctgacatta tgcttaacct tacttaaagg aagagaaact gagacttcac
     1861 cggtcaaaat gaatctacca ggactgaccc tgttagtgct cggtgttggt ggcttgcaaa
     1921 ttatgcttga taaagggcgc gatctggatt ggttcaactc gagtacaata ataatattaa
     1981 cagtagtatc agttatttct ctgatctctt tagtcatttg ggagtcgacc tcagagaacc
     2041 cgattcttga tctcagtttg tttaagtccc gtaacttcac cattggtatt gtgagtatca
     2101 catgcgcgta tttattttac tctggagcga tcgttcttat gccgcagtta ctccaggaaa
     2161 cgatggggta taatgcgata tgggccggac ttgcttatgc gcccatcggc atcatgccac
     2221 tattaatttc acctttgata ggacgttatg gcaacaaaat agacatgcgg ttgttagtga
     2281 catttagttt tttgatgtat gcggtttgct attactggcg ttctgtgaca tttatgccaa
     2341 cgattgattt tacaggcatc attttgccgc agttttttca gggattcgcc gttgcctgtt
     2401 tctttttacc cttaacaacg atttcgtttt caggcttgcc agataataaa tttgccaatg
     2461 cctcgagtat gagtaatttt tttcgtacct tgtcaggatc agttggtacg tcgttgacaa
     2521 tgacgctgtg gggacgacgc gaatcgttac accatagtca gttgacagca accatcgatc
     2581 aatttaaccc cgtgtttaat tcatcgtcac aaattatgga taaatactat ggttcgcttt
     2641 caggagttct taatgaaatt aataatgaaa taacccagca gtcactttct atttctgcaa
     2701 atgagatttt ccgtatggcg gctattgctt ttatcttact tacggttttg gtttggtttg
     2761 cgaaaccgcc gtttacagcg aaaggcattg ggtgatgata aaaggagggg gttatagcgg
     2821 gtaagaccgg ggatcttaag tctgcgccat cggcagcatc gctcacccag ggaaaggatt
     2881 gcgatgctgc gttgaaacgt tattaacggc cttttgccag atattgattc atctcttctt
     2941 ccggcaccat tccacctccc gtcgcccaca ccagatgagt ggtattacgc agttgttctg
     3001 cgctgaaacc gtgcatctgt tggtaactta ctgatgcaca cacgcgctga ggtccggcca
     3061 tacccgccag tgccgaaggt tcaagacgaa taccttcttc ctgcgccagc cagccaagca
     3121 tgtcatacat ggtttgatcg ctaagggtat agaagccatc cagcagacgc tccattgccc
     3181 gcccgacaaa gcctgatgcg cgaccaactg caaggccatc cgctgcggta aggttgtcga
     3241 taccaatatc ctgaacagaa atctgatcgt gtaatcctgt atggacgcct aacaacatac
     3301 aaggggagtg cgttggttcg gcaaaaaagc agtgaacatg atcgccaaac gccagtttaa
     3361 gcccgaatgc gacgccacca ggaccaccgc caacaccaca cggcagatag acaaacagag
     3421 ggttatcagc atcgacgata cggccttgct gggcaaattg cgctttaaga cgctggccag
     3481 cgacggaata cccaaggaac aacgtgcggg aattttcgtc atcaataaag aaacagttcg
     3541 ggtcagactg cgctgcttta cgtccttcct cgacggcaac accataatct tgctcatatt
     3601 ccacgaccgt aacgccatgg ctgcgcagtt tcgctttttt ccatgcccgg gcatcagcag
     3661 acatatgaac tgtcacctta aagccaatgc gggcgctcat aatgccgatt gataacccca
     3721 gatttccggt tgagcccaca gcaatgctgt attggctaaa gaactgttta aactccggag
     3781 aaagcagttt gctgtagtca tcatcaagcg tcagcaaccc cgcttccaga gccagttttt
     3841 ctgcgtgtgc caggacttca taaatcccgc cgcgtgcttt tatggagccg gaaatgggca
     3901 aatggctatc ttttttcagt aacagttgcc cgctgatcgg ttgctgatat tctttttcca
     3961 gccgtttttg catagctgga atggcaacca gttctgattc aataatcccc ccagtggcag
     4021 cagtttcagg aaatgctttt gccagatagg gtgcaaaacg ggataagcgc gcatgggcgt
     4081 cctgaacatc ctgttcggtc aggccaacat aaggtaaacc ttcagccaat gaggtcgtgc
     4141 caggattaaa ccaggtggtt tctttaagag caaccagatc ctttaccaac ggatactggg
     4201 cgatgagcga gttcatttta gcgttttcca tagtacgtct ctttgcgctt agatgataaa
     4261 tgacagcagg aatgtgcccg ccagagcgac gactgaagcg ataaatgtcg ctgtcgtata
     4321 gtatttaaat gtttcattga gcgtagcgcc gcaatattgc ttcactagcc agaaaagcga
     4381 gtccgtaacg atcgtgcagc caattgcacc tgaaccgata gcaatcgcaa taatttccgg
     4441 gctgatgtcg ggatacagcg gcagcacaat gatccatatt tatattttct tctccaggtt
     4501 ggcgatgcca gttggcatgt tttacttact tgcctatatg gttactcgtt ggcgagaaca
     4561 gaggcggaat tgagtacaat tatccggcaa ttattgttat tttttattgt attgccgtgt
     4621 cgggtgatag ggcgttgaga atatcgttgg atctcgccag tgacgtctgt ttctgctaca
     4681 gtgcccgttt tacggcaaac ggcttgggta tggcttcatt aaaggatgtc gcacgcctgg
     4741 cgggagtgtc gatgatgaca gtctcccggg tgatgcataa tgcagaatct gtgcgtcctg
     4801 caacgcgtga ccgcgtattg caggcaatcc agaccctgaa ttatgttcct gatctttccg
     4861 cccgtaagat gcgcgctcaa ggacgtaagc cgtcgactct cgccgtgctg gcgcaggaca
     4921 cggctaccac tcctttctct gttgatattc tgcttgccat tgagcaaacc gccagcgagt
     4981 tcggctggaa tagtttttta atcaatattt tttctgaaga tgacgctgcc cgcgcggcac
     5041 gtcagctgct tgcccaccgt ccggatggca ttatctatac tacaatgggg ctgcgacata
     5101 tcacgctgcc tgagtctctg tatggtgaaa atattgtatt ggcgaactgt gtggcggatg
     5161 acccagcgtt acccagttat atccctgatg attacactgc acaatatgaa tcaacacagc
     5221 atttgctcgc ggcgggctat cgtcaaccgt tatgcttctg gctaccggaa agtgcgttgg
     5281 caacagggta tcgtcggcag ggatttgagc aggcctggcg tgatgctgga cgagatctgg
     5341 ctgaggtgaa acaatttcac atggcaacag gtgatgatca ctacaccgat ctcgcaagtt
     5401 tactcaatga ccacttcaaa tcgggcaaac cagattttga tgttctgata tgtggtaacg
     5461 atcgcgcagc ctttgtggct tatcaggttc ttctggcgaa gggggtacga atcccgcagg
     5521 atgtcgccgt aatgggcttt gataatctgg ttggcgtcgg gcatctgttt ttaccgccgc
     5581 tgaccacaat tcagcttcca catgacatta tcgggcggga agctgcattg catattattg
     5641 aaggtcgtga agggggaaga gtgacgcgga tcccttgccc gctgttgatc cgttgttcca
     5701 cctgatatta tgttaaccca gtagccagag tgctccatgt tgcagcacag ccactccgtg
     5761 ggaggcataa agcgacagtt cccgttcttc tggctgcgga tagattcgac tactcatcac
     5821 cgcttccccg tcgttaataa atacttccac ggatgatgta tcgataaata tccttagggc
     5881 gagcgtgtca cgctgcggga ggggaatact acggtagccg tctaaattct cgtgtgggta
     5941 ataccgccac aaaacaagtc gctcagattg gttatcaata tacagccgca ttccagtgcc
     6001 gagctgtaat ccgtaatgtt cggcatcact gttcttcagc gcccactgca actgaatctc
     6061 aactgcttgc gcgttttcct gcaaaacata tttattgctg attgtgcggg gagagacaga
     6121 ttgatgctgc tggcgtaacg actcagcttc gtgtaccggg cgttgtagaa gtttgccatt
     6181 gctctctgat agctcgcgcg ccagcgtcat gcagcctgcc catccttcac gttttgaggg
     6241 cattggcgat tcccacatat ccatccagcc gataacaata cgccgaccat ccttcgctaa
     6301 aaagctttgt ggtgcataaa agtcatgccc gttatcaagt tcagtaaaat gcccggattg
     6361 tgcaaaaagt cgtcctggcg accacattcc gggtattacg ccactttgaa agcgatttcg
     6421 gtaactgtat ccctcggcat tcattccctg cggggaaaac atcagataat gctgatcgcc
     6481 aaggctgaaa aagtccggac attcccacat atagctttca cccgcatcag cgtgggccag
     6541 tacgcgatcg aaggtccatt cacgcaacga actgccgcga taaagcagga tctgccccgt
     6601 gttgcctgga tctttcgccc cgactaccat ccaccatgtg tcggcttcac gccacacttt
     6661 aggatcgcgg aagtgcatga ttccttctgg tggagtgagg atcacaccct gtttctcgaa
     6721 atgaatacca tcccgactgg tagccagaca ttgtacttcg cgaattgcat cgtcattacc
     6781 tgcaccatcg agccagacgt gtccggtgta gataagtgag aggacaccat tgtcatcgac
     6841 agcactacct gaaaaacacc cgtctttgtc attatcgtct cctggcgcta gcgcaatagg
     6901 ctcatgctgc cagtggatca tatcgtcgct ggtggcatgt ccccagtgca ttggtcccca
     6961 gtgttcgctc atcgggtgat gttgataaaa cgcgtgataa cgatcgttaa accagatcag
     7021 gccgtttgga tcgttcatcc atccggcagg tggcgcgagg tgaaaatggg gatagaaagt
     7081 gttaccccgg tgctcatgaa gttttgctag tgcgttttgc gccgcatgca atcgagattg
     7141 cgtcatttta atcatcctgg ttaagcaaat ttggtgaatt gttaacgtta acttttataa
     7201 aaatatagtc ccttactttc ataaatgcga tgaatatcac aaatgttaac gttaactatg
     7261 acgttttgtg atcgaatatg catgttttag taaatccatg acgattttgc gagaaagagg
     7321 tttatcacta tgcgtaactc agatgaattt aagggaaaaa aatgtcagcc aaagtatggg
     7381 ttttagggga tgcggtcgta gatctcttgc cagaatcaga cgggcgccta ctgccttgtc
     7441 ctggcggcgc gccagctaac gttgcggtgg gaatcgccag attaggcgga ataagtggat
     7501 ttataggtcg ggtcggtgat gatccttttg gggcgttaat gcaaagaacg ctgctaactg
     7561 agggagtcga tatcacgtat ctgaagcaag atgaatggca ccggacatcc acggtgcttg
     7621 tcgatctgaa cgatcaaggg gaacgttcat ttacgtttat ggtccgcccc agtgccgatc
     7681 tttttttaga gacgacagac ttgccctgct ggcgacatgg cgaatggtta catctctgtt
     7741 caattgcgtt gtctgccgag ccttcgcgta ccagcgcatt tactgcgatg acggcgatcc
     7801 ggcatgccgg aggttttgtc agcttcgatc ctaatattcg tgaagatcta tggcaagacg
     7861 agcatttgct ccgcttgtgt ttgcggcagg cgctacaact ggcggatgtc gtcaagctct
     7921 cggaagaaga atggcgactt atcagtggaa aaacacagaa cgatcaggat atatgcgccc
     7981 tggcaaaaga gtatgagatc gccatgctgt tggtgactaa aggtgcagaa ggggtggtgg
     8041 tctgttatcg aggacaagtt caccattttg ctggaatgtc tgtgaattgt gtcgatagca
     8101 cgggggcggg agatgcgttc gttgccgggt tactcacagg tctgtcctct acgggattat
     8161 ctacagatga gagagaaatg cgacgaatta tcgatctcgc tcaacgttgc ggagcgcttg
     8221 cagtaacggc gaaaggggca atgacagcgc tgccatgtcg acaagaactg gaatagtgag
     8281 aagtaaacgg cgaagtcgct cttatctcta aataggacgt gaatttttta acgacaggta
     8341 ggtaattatg gcactgaata ttccattcag aaatgcgtac tatcgttttg catccagtta
     8401 ctcatttctc ttttttattt cctggtcgct gtggtggtcg ttatacgcta tttggctgaa
     8461 aggacatcta gggttgacag ggacggaatt aggtacactt tattcggtca accagtttac
     8521 cagcattcta tttatgatgt tctacggcat cgttcaggat aaactcggtc tgaagaaacc
     8581 gctcatctgg tgtatgagtt tcatcctggt cttgaccgga ccgtttatga tttacgttta
     8641 tgaaccgtta ctgcaaagca atttttctgt aggtctaatt ctgggggcgc tcttttttgg
     8701 cctggggtat ctggcgggat gcggtttgct tgacagcttc accgaaaaaa tggcgcgaaa
     8761 ttttcatttc gaatatggaa cagcgcgcgc ctggggatct tttggctatg ctattggcgc
     8821 gttctttgcc ggcatatttt ttagtatcag tccccatatc aacttctggt tggtctcgct
     8881 atttggcgct gtatttatga tgatcaacat gtgttttaaa gataaggatc accagtgcgt
     8941 agcggcggat gcgggagggg taaaaaaaga ggattttatc gcagttttca aggatcgaaa
     9001 cttctgggtt ttcgtcatat ttattgtggg gacgtggtct ttctataaca tttttgatca
     9061 acaacttttt cctgtctttt atgcaggttt attcgaatca cacgatgtag gaacgcgcct
     9121 gtatggttat ctcaactcat tccaggtggt actcgaagcg ctatgcatgg cgattattcc
     9181 tttctttgtg aatcgggtag ggccaaaaaa tgcattactt atcggtgttg tgattatggc
     9241 gttgcgtatc ctttcctgcg cgctgttcgt taacccctgg attatttcat tagtgaagct
     9301 gttacatgct attgaggttc cactttgtgt catatccgtc ttcaaataca gcgtggcaaa
     9361 ttttgataag cgcctgtcgt cgacgatctt tctgattggt tttcaaattg ccagttcgct
     9421 tgggattgtg ctgctttcaa cgccgactgg gatactcttt gaccacgcag gctaccagac
     9481 agttttcttc gcaatttcgg gtattgtctg cctgatgttg ctatttggca ttttcttctt
     9541 gagtaaaaaa cgcgagcaaa tagttatgga aacgcctgta ccttcagcaa tatagacgta
     9601 aactttttcc ggttgttgtc gatagctcta tatccctcaa ccggaaaata ataatagtaa
     9661 aatgcttagc cctgctaata atcgcctaat ccaaacgcct cattcatgtt ctggtacagt
     9721 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagt
     9781 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     9841 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     9901 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     9961 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
    10021 tcgtttgaag ggtatctggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
    10081 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
    10141 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gctgggtttt
    10201 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
    10261 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
    10321 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
    10381 gcgtgccgtt aaacaccaga tagaggattt caacagaacc gacaacgatg tgggtggtgt
    10441 cacccagtgc aataagccag gtcatcaata taatcaccac aatctttgcc gcacccgcag
    10501 caggaaacat ccaaaccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
    10561 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
    10621 cttcattgaa gataggcata tattcaaatg cccatgcagc aatacctgtc ccgagaatat
    10681 tacccagcag cacgacgccc cataatcgca taagtaagcc gacgttgctc attgtcggtt
    10741 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
    10801 taatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcctggca
    10861 ctccttccag ttcgacatga aatatccctt ttgccagtag cgaagcgccc atcgacagac
    10921 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
    10981 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
    11041 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
    11101 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
    11161 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
    11221 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
    11281 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
    11341 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
    11401 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
    11461 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
    11521 cgcaccatgt acaacttcaa cttcaatgta ttagacccgt atattgttcg accggtcgct
    11581 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg gtttgagcaa ctttactggc
    11641 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
    11701 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ttttattgat
    11761 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
    11821 cttggtcatt atggcgtggg ttatgggcct tacgtccagt taccgttcta cggtagcttc
    11881 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
    11941 acctggccga tgtctgtggg taaatggacg cttgaaggga tagaaactcg tgcgcagttg
    12001 ttggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
    12061 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
    12121 aacgcgcaag cgattcagga tgatttaaaa gatatcgatt ctgaataaga aacaaataaa
    12181 aaaggtgagt cgcaatactc accttttttg ttgttatccc tcaattacca caagtaaaag
    12241 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
    12301 tatttccgat attcaggagt tgaaaaatca aaaaactgga aaaataattt taaatatcat
    12361 caaaattaac acctggcg
//
LOCUS       DAPHJE010000123        12212 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPHJE010000123 DAPHJE010000000
VERSION     DAPHJE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.124"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..918
                     /locus_tag="RH222_003659"
     CDS             49..918
                     /locus_tag="RH222_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV3798615.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGNKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLVGMPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1111..1464)
                     /locus_tag="RH222_003660"
     CDS             complement(1111..1464)
                     /locus_tag="RH222_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV3798616.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1602..3248)
                     /gene="groL"
                     /locus_tag="RH222_003661"
     CDS             complement(1602..3248)
                     /gene="groL"
                     /locus_tag="RH222_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV3798617.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3292..3585)
                     /locus_tag="RH222_003662"
     CDS             complement(3292..3585)
                     /locus_tag="RH222_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV3798618.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3861..5117
                     /gene="yjeH"
                     /locus_tag="RH222_003663"
     CDS             3861..5117
                     /gene="yjeH"
                     /locus_tag="RH222_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV3798619.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(5133..5609)
                     /gene="fxsA"
                     /locus_tag="RH222_003664"
     CDS             complement(5133..5609)
                     /gene="fxsA"
                     /locus_tag="RH222_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV3798620.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5946..7382
                     /gene="aspA"
                     /locus_tag="RH222_003665"
     CDS             5946..7382
                     /gene="aspA"
                     /locus_tag="RH222_003665"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV3798621.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7500..8801
                     /gene="dcuA"
                     /locus_tag="RH222_003666"
     CDS             7500..8801
                     /gene="dcuA"
                     /locus_tag="RH222_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV3798622.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML"
     gene            8917..9255
                     /gene="cutA"
                     /locus_tag="RH222_003667"
     CDS             8917..9255
                     /gene="cutA"
                     /locus_tag="RH222_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV3798623.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9231..10928
                     /gene="dsbD"
                     /locus_tag="RH222_003668"
     CDS             9231..10928
                     /gene="dsbD"
                     /locus_tag="RH222_003668"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV3798624.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10965..11540
                     /locus_tag="RH222_003669"
     CDS             10965..11540
                     /locus_tag="RH222_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3798625.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11647..11722
                     /locus_tag="RH222_003670"
     tRNA            11647..11722
                     /locus_tag="RH222_003670"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11680..11682,aa:Phe,seq:gaa)
BASE COUNT         2953 a         3138 c         2998 g         3123 t
ORIGIN      
        1 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
       61 atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc attggcgctg
      121 aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat ggaactgcgc
      181 gacctgctga ttgcgctgga aagtcgtctg catcaaaaac ataaactgga tgctgctgca
      241 cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggcaga aaacatccca
      301 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
      361 cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga cggtaacaaa
      421 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
      481 attaataatg cggaaatgcg tgaactggca ttacgtatta cctcactgtt agatttcctg
      541 ccgttatatg acgttgactg ccaggaaaat ggcaatctgg aatatgacac ctattcccaa
      601 ccggaatgga aacataactt gtttgaccat tatctggcgg tgctttaccg ctttaaagat
      661 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
      721 aaagaagtag aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactc
      781 gtgggtatgc catgtcaggt ctatgttcgc accgtagcgg cgaataacgc ccaaccgcta
      841 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
      901 gcaccacagg cgaaataatc tcatctttgc taatgcgagg ctggagccat ccagcctgaa
      961 atgactgata acgtgatcgt tggtcatgcc ggatgcggcg taaacgcctt atccggccta
     1021 caaattctca agcattcaat cagttgcaat gcattatgta ggcctgataa gcgaagcgca
     1081 tctggcaatt atacgtttgt caccatcaca tcagttcggg cacttataaa cctggccgat
     1141 aatctggcta tccgtcggga caaaactgga caacattccc tgcgacgggc tactgatgcc
     1201 ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc agatcgtttg ctgcgccgcg
     1261 catagaaccg ccttcttctc cgtgttgccc ggaaagccag ttgctttgct tacctgtcgc
     1321 agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc acaatgcgta cactctgacc
     1381 ggcagcactc aattcgttgc tggagctaca acccgccatc agtagcgcgg caccgacaat
     1441 ccctgctaag tattttacgt gcatgttatt ccccataatc aatgagctgg acgccactcg
     1501 cgtcgtccgt gtctgaatct tatactagaa agatgaccaa aagaaaaacc cccagacatt
     1561 tctgcccggg ggtttgttta tttctgcgag gtgcagggca attacatcat gccgcccatg
     1621 ccacccatgc cgcccatacc gccagcagcg cctaagtcag ctgcatcatt tttcggcagg
     1681 tcggtaacca tgcactcggt ggtgatcatc aggccagcca cagaagccgc gtactgcaga
     1741 gcagaacggg ttactttggt tgggtccagg atacccatgt cgatcatgtt gccgtattct
     1801 tcggttgctg cgttgtaacc gtagttgccg tcgccgcctt taacggtgtt agcaacaaca
     1861 gacggttctt cgccgcagtt caggacgatc tgacgcagcg gagcttccat tgcacgcagt
     1921 gcaactttga tacccacgtt ctggtcttcg ttctgaccac gcaggtcagc cagtttagac
     1981 gctacgcgga tcagcgcaac accaccacca gcaaccacgc cttcttctac cgcagcacgg
     2041 gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt tcatttcaac ttcggtagca
     2101 gcacctactt tgataactgc aacgccgcct gccagtttcg ctacgcgttc ctgcagtttt
     2161 tcacggtcgt agtcagaagt tgcttcttca atctgctgac ggatctgagc aacacggccc
     2221 tggattgcag cttcttcacc cacgccatcg atgatagtgg tggtgtcttt gttgatcaca
     2281 acacgtttag cctgacccag gtcttccagg gttgcttttt ccagctccat accgatctct
     2341 tcagagatta cggtaccgcc agtcagggtt gcgatatcct gcagcatagc tttacgacga
     2401 tcgccgaagc ccggagcttt aaccgcagca actttcacga tgccacgcat ggtgttaaca
     2461 accagagttg ccagcgcttc gccttcaaca tcttcagcga tgatcagcag cggtttgcct
     2521 gctttggcaa cagcttccag aaccggcagc atttcgcgga tgttagagat tttcttgtca
     2581 gccagcagga tgaacgggct ttccagttct actgcgccag tttccggctt gttgatgaag
     2641 taaggagaca ggtagccacg gtcgaactgc ataccttcaa ccacgtccag ttcgtcctgc
     2701 agaccggtac cgtcttcaac ggtgataacg ccttctttac cgactttgtc catcgcttca
     2761 gcgatcagtt tacctacggt ttcgtcggag ttagcggaga tggtaccaac ctgagcaatc
     2821 gctttagagt cagagcacgg tacggacagc gctttcagtt cttcaactgc agcggtaacg
     2881 gctttgtcga taccacgttt caggtccatc gggttcatgc ccgcagcaac agctttcaga
     2941 ccctcagtga tgatagcctg agccagtaca gttgcagtgg tggtaccgtc gcctgcagcg
     3001 tcgttcgctt tagaggcaac ttctttcacc atctgcgcac ccatattttc gaacttgtct
     3061 tccagttcga tttcacgagc aacggaaaca ccatctttgg tgatggtcgg tgcaccgaaa
     3121 gatttatcca gaactacgtt acggcctttc ggaccgaggg taactttcac tgcatctgcc
     3181 agtacgttta cgccgcgcag cattttcaca cgagcgtcgt taccgaattt tacgtcttta
     3241 gctgccatta tctttattcc ttaaattcgt atgttcagtg tcgtgcgagg attacgcttc
     3301 aacaattgcc agaatgtcgc tttcggacat gatcaacact tcttcattgt cgatcttctc
     3361 agatttcaca ccgtagccat cgttgaaaat aacgatgtcg ccaactttca catccagcgg
     3421 cttcacttcg ccattttcaa ggatacggcc attgccgaca gccagcactt cgccgcgggt
     3481 ggatttagcc gctgcagagc cggtcagaac gatgccgcca gcagatttag tttcaacttc
     3541 tttacgcttg acgatcacgc gatcatgcaa tggacgaata ttcattgata actctccttt
     3601 gagaaagtcc gtatctgtta tgggtgacgc cggagcttac gtggtttccc ggctggtgac
     3661 cagagaaatg gggatgagga ttcgcccctt caagggggaa aaagaaaaaa aattctgatc
     3721 agcacaaaat cgggtgaaaa ccctgattca cctcacattt catcgcaatt tcttcatcgc
     3781 cgtcataagc gaatctgatt gtgctaccat cgaaaatcta cgcagttgcc caaaatttgg
     3841 gcgcaatcgg atatcaaccc atgagtggac tcaaacaaga actggggctg gcccagggca
     3901 ttggactgct atcgacgtca ttattaggca ctggcgtgtt tgccgttcct gcgttagctg
     3961 cgctggtagc gggcaataac agcctgtggg cgtggcccgt tttgattatc ttagtgttcc
     4021 cgattgcgat tgtgtttgcg attctgggtc gccactatcc cagcgcaggc ggtgtcgcgc
     4081 acttcgtcgg tatggcgttt ggttcgcgac ttgagcgagt caccggctgg ttgtttttat
     4141 cggtcattcc cgtgggtttg cctgccgcgc tacaaattgc cgccgggttc ggccaggcaa
     4201 tgtttggctg gcatagctgg caactgttgt tggcagaact cggtacgctg gcgctggtgt
     4261 ggtatatcgg tactcgcggt gccagttcca gtgctaatct acaaaccgtt attgccggac
     4321 ttatcgtcgc gctgattgtc gctatctggt gggcgggcga tatcaaacct gcgaatatcc
     4381 ccttcccggc acctggtaat atcgaactta ccgggttatt tgctgcgtta tcagtgatgt
     4441 tctggtgttt tgtcggtctg gaagcatttg cccatcttgc ctcggaattt aaaaatccag
     4501 agcgtgattt tcctcgcgca ttgatgattg gtctgctgct ggcaggatta gtctactggg
     4561 gctgtacggt agtcgtctta cacttcgacg cctatggtga aaaaatggcg gcggcagcat
     4621 cgcttccaaa aattgtagtg cagttgttcg gtgtaggagc gttatggatt gcctgcgtga
     4681 ttggctatct ggcctgcttt gccagtctca acatttatat acagagcttc gcccgcctgg
     4741 tctggtcgca ggcgcaacat aatcctgacc actacctggc acgcctctct tctcgccata
     4801 tcccgaataa tgccctcaat gcggtgctcg gctgctgtgt ggtgagcacg ttggtgattc
     4861 atgctttaga gatcaatctg gacgctctta ttatttatgc caatggcatc tttattatga
     4921 tttatctgtt atgcatgctg gcaggctgta aattattgca aggacgttat cgactactgg
     4981 cggtggttgg cgggctgtta tgcgttctgt tactggcaat ggtcggctgg aaaagtctct
     5041 atgcgctgat catgctggcg gggttatggc tgttgctgcc aaaacgaaaa acgccggaaa
     5101 atggcataac cacataatcc ggcgtttcga cattaatcct ggcgatcgtc tttatgatca
     5161 aggcggtcgc gctcatcatc ctttcgctgg tactcaccat caaaagtatt accgccaccg
     5221 gtcccggcgc taaaaccgcc gcctggcata cgagaaaagc gcaaatgcgg catcaacttc
     5281 actgtcagat gcttttgcac cggcggtaat aaaagcagaa gaccgaggaa gtcggtgaaa
     5341 aagcccggta ataaaagcag caaaccggca atgatcagcg aaacactttt aatcatctcc
     5401 gccgctgggt tttcacccgc cgccattttt tgctgcatca gcacgaaatt cttaaagccc
     5461 tggttacgta ccagtgacat cccgataact gacgtgaata taaccagcac gagggtcagc
     5521 aataccccca atacatgggc aacctgaata aagattgaaa tctcaatata gacataaagg
     5581 aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct gtaatagcag ccggtaaaac
     5641 ccggctacct gaatgggttg cgaatcgcgt tcagcttata ttgtggtcat tagcaaaatt
     5701 tcaagatgtt tgcgcaacta tttttggtag taatcccaaa gcggtgatct atttcacaaa
     5761 ttaataatta aggggtaaaa accgacactt aaagtgatcc agattacggt agaaatcctc
     5821 aagcagcata tgatctcggg tattcggtcg atgcagggga taatcgtcgg tcgaaaaaca
     5881 ttcgaaacca catatattct gtgtgtttaa agcaaatcat tggcagcttg aaaaagaagg
     5941 ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt gggtaccagg gaagttccag
     6001 ctgatgccta ctatggtgtt cacactctga gagcgattga aaacttctat atcagcaaca
     6061 acaaaatcag tgatattcct gaatttgttc gcggtatggt aatggttaaa aaagccgcag
     6121 ctatggcaaa caaagagctg caaaccattc ctaaaagtgt agcgaatgcc atcattgccg
     6181 catgtgatga agtcctgaac aacggaaaat gcatggatca gttcccggta gacgtctacc
     6241 agggcggcgc aggtacttcc gtaaacatga acaccaacga agtgctggcc aatatcggtc
     6301 tggaactgat gggtcaccag aaaggtgaat atcagtacct gaacccgaac gaccatgtta
     6361 acaaatgtca gtccactaac gacgcctacc cgaccggttt ccgtatcgca gtttactctt
     6421 ccctgattaa gctggtagat gcgattaacc aactgcgtga aggctttgaa cgtaaagctg
     6481 tcgaattcca ggacatcctg aaaatgggtc gtacccagct gcaggacgca gtaccgatga
     6541 ccctcggtca ggaattccgc gctttcagca tcctgctgaa agaagaagtg aaaaatatcc
     6601 aacgtaccgc tgaactgctg ctggaagtta accttggcgc aacagcaatc ggtactggtc
     6661 tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa actggctgaa gtcactggct
     6721 tcccatgcgt accggctgaa gacctgatcg aagcgacctc tgactgcggc gcttatgtta
     6781 tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc caaaatctgt aacgacctgc
     6841 gcttgctctc ttctggtcca cgtgccggcc tgaacgagat caacctgccg gaactgcagg
     6901 cgggctcttc catcatgcca gctaaagtaa acccggttgt tccggaagtg gttaaccagg
     6961 tatgcttcaa agtcatcggt aacgacacca ctgttaccat ggcagcagaa gcaggtcagc
     7021 tacagttgaa cgttatggag ccggtcattg gccaggccat gttcgaatcc gttcacattc
     7081 tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa cggcatcact gctaacaaag
     7141 aagtgtgcga aggttacgtt tacaactcta tcggtatcgt tacttacctg aacccgttca
     7201 tcggtcacca caacggtgac atcgtgggta aaatctgtgc cgaaaccggt aagagtgtac
     7261 gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga acttgacgat attttctccg
     7321 tacagaatct gatgcacccg gcttacaaag caaaacgcta tactgatgaa agcgaacagt
     7381 aatcgtacag ggtagtacaa ataaagaagg cacgtcagat gacgtgcctt ttttcttgtg
     7441 agcagtaact taaaaataac aacctaatat caacttgtta aaaaacaagg aaggctaata
     7501 tgctagttgt agaactcatc atagttttgc tggcgatctt cttgggcgcc agattggggg
     7561 gaataggtat tggttttgca ggcggattgg gggtgctggt tcttgccgct attggcgtta
     7621 aacccggtaa catcccgttc gatgtcattt ccattatcat ggcggttatc gccgctattt
     7681 ctgccatgca ggttgctggc ggtctggact atctggttca tcagacagaa aagctgctgc
     7741 gccgtaaccc gaaatacatc acgatcctcg caccgatcgt gacctatttc ctgactatct
     7801 ttgctggtac tggcaacata tctctggcga cactgccagt tatcgctgaa gttgcgaagg
     7861 aacaaggcgt caaaccttgc cgtccgctgt ctactgcagt ggtatccgcg cagattgcga
     7921 tcaccgcatc gccaatctca gcggcagtgg tttacatgtc ttccgtgatg gaaggtcatg
     7981 gcatcagcta cctccatctg ctctccgtgg tcatcccgtc caccctgctg gcggttctgg
     8041 tgatgtcctt cctggtcact atgctgttca actccaaact ctctgacgat ccgatttatc
     8101 gtaagcgtct ggaagagggc ctggttgaac tgcgcggtga aaagcagatt gaaatcaaat
     8161 ccggtgcaaa aacgtccgtc tggctgttcc tgctgggcgt agttggcgtg gttatctatg
     8221 caatcatcaa cagcccaagc atgggtctgg ttgaaaaacc gctgatgaac accaccaacg
     8281 caatcctgat catcatgctc agcgttgcaa ctctgaccac cgttatctgt aaagtcgata
     8341 ccgacaacat cctcaactcc agcaccttca aagcaggtat gagcgcctgt atttgtatcc
     8401 tgggtgttgc gtggctgggc gatactttcg tttccaacaa catcgactgg atcaaagata
     8461 ccgctggtga agtgattcag ggtcatccgt ggctgctggc cgtcatcttc ttctttgctt
     8521 ctgctctgct gtactctcag gctgcaaccg caaaagcact gatgccgatg gctctggcac
     8581 tgaacgtttc tccgctgacc gctgttgctt ctttcgctgc ggtgtctggt ctgttcattc
     8641 tgccaaccta cccgacgctg gttgctgcgg tacagatgga tgacacgggt actacccgta
     8701 tcggtaaatt cgtcttcaac catccgttct tcatcccggg tactctgggt gttgccctgg
     8761 ccgtttgctt cggcttcttg ctgggtagct tcatgctgta atgaccaatc gcggggcgtt
     8821 cacgccccgc tttctttccc gccgactaac atccttcccc cgtccgttgt atagtgacct
     8881 ctctcttgcg gttccatctg ttcttgcgag gtgtttatgc ttgatgaaaa aagttcgaat
     8941 accgcgtctg tcgtggtgct atgtaccgca ccggatgaag cgacagccca ggatttagcc
     9001 gccaaagtgc tggcggaaaa actggcggcc tgcgcgacct tgatccccgg cgctacctct
     9061 ctctattact gggaaggtaa gctggagcaa gaatacgaag tgcagatgat tttaaaaact
     9121 accgtatctc accagcaggc actgctggaa tgcctgaagt ctcatcatcc atatcaaacc
     9181 ccggaacttc tggttttacc tgttacacac ggagacacag attacctctc atggctcaac
     9241 gcatctttac gctgatcctg ctactttgca gcacttccgt ttttgccgga ttattcgacg
     9301 cgccgggacg ttcacaattt gtccccgcgg atcaagcctt tacttttgat tttcagcaaa
     9361 accaacatga ccttaatctg acctggcaga tcaaagacgg ttactacctc taccgtaaac
     9421 agatccgcat tacgccggaa cacgcgaaaa ttgccgacgt gcagctgccg caaggcgtct
     9481 ggcatgaaga tgagttttac ggcaaaagcg agatttaccg cgatcggctg acgcttcccg
     9541 taaccatcaa ccaggcgagt gcgggagcga cgttaactgt cacctaccag ggctgcgctg
     9601 atgccggttt ctgttatccg ccagaaacca aaaccgttcc gttaagcgaa gtggtcgcca
     9661 acaacgcagc gtcacagcct gtgtctgttt cgcagcaaga gcagcacacc gcgcaattgc
     9721 ccttttccgc gctctgggcg ttgttgatcg gtattggtat cgcctttacg ccatgcgtgc
     9781 tgccaatgta cccactgatt tctggcatcg tgctgggtgg taaacagcgg ctctccactg
     9841 ccagagcatt gttgctgacc tttatttatg tgcaggggat ggcactgact tacacggcgc
     9901 tgggtctggt agttgccgcc gcagggttac agttccaggc ggcgctacag cacccatacg
     9961 tgctcattgg cctcgccatc gtctttacct tgctggcgat gtcaatgttt ggcttgttta
    10021 ctctacaact cccctcttcg ctgcaaacac gtctcacgtt gatgagcaat cgccaacagg
    10081 gcggctcacc tggcggtgtg tttgttatgg gggcgattgc cggactgatc tgttcaccat
    10141 gcaccaccgc accgcttagc gcgattctgc tgtatatcgc ccaaagcggg aacatgtggc
    10201 tgggcggcgg cacgctttat ctctatgcgt tgggcatggg cctgccgctg atgctaatta
    10261 ccgtctttgg taaccgcttg ctgccgaaaa gcggcccgtg gatggaacaa gtcaaaaccg
    10321 cgtttggttt tgtgatcctc gcactgccgg tcttcctgct ggagcgagtg attggtgatg
    10381 tatggggatt acgcttgtgg tcggcgctgg gtgtcgcatt ctttggctgg gcctttatca
    10441 ccagcctaca ggctaaacgc ggctggatgc gcgtggtgca aataatcctg ctggcagcgg
    10501 cattggttag cgtgcgccca cttcaggatt gggcatttgg tgcgacgcat accgcgcaaa
    10561 ctcagacgca tctcaacttt acacaaatca aaacggtaga tgagttaaat caggcgctcg
    10621 ttgaagccaa aggcaaaccg gtgatgttag atctttatgc cgactggtgc gtcgcctgta
    10681 aagagtttga gaaatacacc ttcagcgacc cgcaggtgca aaaagcgtta gcagacacgg
    10741 tcttacttca ggccaacgtc acggccaacg acgcacaaga tgtggcgctg ttaaagcatc
    10801 ttaatgtcct tggcctaccg acaattctct tttttgacgg acaaggccag gagcatccac
    10861 aagcacgcgt cacgggcttt atggatgctg aaaccttcag cgcacatttg cgcgatcgcc
    10921 aaccgtgaac gacacttgca gtgggaaaga cggaggaaaa tagcgtgcaa cgtgaagatg
    10981 tactgggaga agccctgaaa ttattagaat tacaagggat tgccaacacc acgctggaga
    11041 tggttgctga acgtgtggat tatccactgg acgagctacg ccgcttctgg ccagacaaag
    11101 aggcaatcct ctacgatgcg ctgcgttatc ttagccaaca gatagatgtc tggcgtcgtc
    11161 agctgatgct ggacgaaacg caaaccgccg aacaaaagtt gctggcacgt tatcaggcgt
    11221 tatcggagtg cgttaaaaac aaccgctatc cgggctgtct atttatcgct gcctgtacgt
    11281 tttatcccga tcctggccac cctattcatc aactggccga tcagcaaaaa agcgcggcct
    11341 acgatttcac ccacgaactg ttaaccacgc tggaagttga cgatccggcg atggtagcaa
    11401 agcagatgga actggtgctg gaaggctgtt taagccgaat gctggtgaat cgtagccagg
    11461 cggatgtcga cactgcacat cggctggcgg aagatatcct gcgcttcgcc cgctgccgcc
    11521 agggtggtgc attgacctga cagaaacaca gaaaagaagc gatttgccgc aatcttaagc
    11581 agttgaatcg cttttactga aattaggttg acgagatgtg cagattacgg tttaatgcgc
    11641 cccgttgccc ggatagctca gtcggtagag caggggattg aaaatccccg tgtccttggt
    11701 tcgattccga gtccgggcac caaattcata tcaacggacc tccacggagg tccgtttttc
    11761 gtttctggac gccacaattt aagcgttctg cctccaaatc aattctcccg aactcaacca
    11821 gattctcccc acatcaccag caatttgcgg gcatatccca attcgggaaa atttgttcct
    11881 gagcgatagc actaactgac gtgaaatgtc gtgcggcccc gtgatgttgt tgaacgtcaa
    11941 atgacgtcat caggagcgta acgcatccat aaagcacaac accggtctga acgccgactg
    12001 atgagatttt ctgaatgaga acaaagagaa atgtatcagt ccgcttgctc atgcaaagac
    12061 taacaatcca ttaaaatagt aagcgctccg gacaattttc catggattat tttctgaaca
    12121 tttttctttg gcaaagatga tgaattttga tggtaaggaa tattacttct ggttctcagt
    12181 aaaatccttt cgtaatacta tgtaatcaag aa
//
LOCUS       DAPHJE010000124        12179 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPHJE010000124 DAPHJE010000000
VERSION     DAPHJE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.125"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            345..1115
                     /locus_tag="RH222_003671"
     CDS             345..1115
                     /locus_tag="RH222_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV3798626.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1106..1579
                     /locus_tag="RH222_003672"
     CDS             1106..1579
                     /locus_tag="RH222_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV3798627.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1687..2226
                     /gene="dnaT"
                     /locus_tag="RH222_003673"
     CDS             1687..2226
                     /gene="dnaT"
                     /locus_tag="RH222_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV3798628.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2229..2966
                     /gene="dnaC"
                     /locus_tag="RH222_003674"
     CDS             2229..2966
                     /gene="dnaC"
                     /locus_tag="RH222_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV3798629.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3015..3509
                     /locus_tag="RH222_003675"
     CDS             3015..3509
                     /locus_tag="RH222_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV3798630.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3763..6054
                     /gene="opgB"
                     /locus_tag="RH222_003676"
     CDS             3763..6054
                     /gene="opgB"
                     /locus_tag="RH222_003676"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV3798631.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDMLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6192..7214)
                     /gene="lgoD"
                     /locus_tag="RH222_003677"
     CDS             complement(6192..7214)
                     /gene="lgoD"
                     /locus_tag="RH222_003677"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV3798632.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7353..8266
                     /locus_tag="RH222_003678"
                     /pseudo
     CDS             7353..8266
                     /locus_tag="RH222_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            8481..9836
                     /locus_tag="RH222_003679"
     CDS             8481..9836
                     /locus_tag="RH222_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3798633.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNVRRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     A"
     gene            complement(9892..11556)
                     /gene="tsr"
                     /locus_tag="RH222_003680"
     CDS             complement(9892..11556)
                     /gene="tsr"
                     /locus_tag="RH222_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV3798634.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPTTPRKMAVADSGENWETF"
     gene            complement(11675..12121)
                     /gene="hpaR"
                     /locus_tag="RH222_003681"
     CDS             complement(11675..12121)
                     /gene="hpaR"
                     /locus_tag="RH222_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDV3798635.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2969 a         3199 c         2952 g         3059 t
ORIGIN      
        1 ataattaaat gatatccctt cccgcagaat gaaaaatcac acaaccaata tttattcaat
       61 gaaatctgat ggttttaact tgttaacatt aaaaaacgtg cccggttatc tattatcctg
      121 attcgtaaag gctaaacaat aaattcatta tgatgataat attcatgtga tagtttgttt
      181 gcggcgagag ataattcgct ttttatcacc aagcgtctcc cgataaccag gattaatcag
      241 cgcaattttg atcttaacct accgcaaaac aaccgtgctt catgatacaa atggattctc
      301 attatctgaa caatacgcat cacgtctatg acaaagggcg agttatgcaa actgagcaac
      361 agcgagccgt aacgcggctt tgtatccagt gtggattatt tcttttgcaa catggtgcgg
      421 aaagcgcgtt ggttgatgag ctttcctcac gactgggtcg ggcgctggga atggacagcg
      481 tcgaaagttc tatctcttcg aacgccatag tgctgacaac tattaaagat gggcaatgcc
      541 tgacatcgac acgtaaaaat cacgatcgcg gcattaatat gcatgtggtg actgaagtcc
      601 agcacattgt gattcttgcg gagcatcatc tgctggatta caaaggcgta gagaaacgat
      661 ttagccaaat tcagccatta cgttacccaa gatggctggt agccttaatg gttggccttt
      721 cttgcgcctg tttctgtaaa ctcaataacg gtggctggga tggtgccgtc atcaccttct
      781 ttgccagtac gaccgcgatg tatatccgcc agctgctggc acaacgtcat cttcatccac
      841 agatcaactt ttgccttacc gctttcgccg ccaccaccat ttccggattg cttttgcaac
      901 tccccacttt cagcaatacc cccaccattg cgatggccgc cagcgttctg ctgctggtgc
      961 cgggctttcc gttgattaat gccgtcgccg atatgtttaa aggccacatc aataccggac
     1021 tggcacgctg ggcgatcgcc agtctgctga cactggctac ctgcgtcggc gtggtgatgg
     1081 cactgacgat ttgggggcta cgcggatggg tgtgatcgaa tttctgtttg cattggcgca
     1141 ggatatgatc ctcgccgcca ttcctgcggt cggctttgcg atggtgttca acgttcccgt
     1201 gcgggcgtta cgctggtgtg cgctgcttgg ctcgataggt catggttccc gaatgatctt
     1261 gatgaccagc gggttgaata ttgagtggtc aacctttatg gcttctatgc tggtcggcac
     1321 cattggtatt caatggtcgc gctggtatct ggcacatccg aaagtgttta ccgtggcggc
     1381 tgttatccct atgtttccgg gcatatcggc ttataccgca atgatttcgg cggtaaaaat
     1441 cagccagcta ggttacagcg aaccgttgat gattaccctg ttaaccaact ttcttacagc
     1501 ttcatcgatt gttggtgcgt tatctatcgg tctttccatt cctggattat ggttgtaccg
     1561 caagcgccct cgcgtataaa attgcctcgc ttaaagcacg cggatgagag acagcctcct
     1621 ctcctccgtg tgttactata aaagttatct cccttctcgt tcatcgttcc atatttgaga
     1681 aacagtatgt cttccagagt tttgaccccg gacgtcgttg gtattgacgc cctggtacac
     1741 gatcaccaaa ccgttctggc aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat
     1801 gccccggcgt tttatgccgt cacgcctgca cgcctggctg aactgctggc gctggaagaa
     1861 aagctggcgc gtccgggaag cgatgtcgct ctggacgatc aactctatca ggaaccgcaa
     1921 gccgctcccg ttgctgtacc catggggaaa ttcgccatgt atccggactg gcaacccgat
     1981 gccgatttta tccgcctggc ggcgctatgg ggcgtggcgc taagagagcc ggtgaccacc
     2041 gaagaactgg cctcattcat tgcctactgg caggcggaag gtaaagtctt tcaccatgtg
     2101 cagtggcaac aaaaactggc gcgcagcctg caaatcggtc gtgccagcaa cggcggactg
     2161 ccgaaacgag atgtgaatac ggtcagtgaa cctgacagcc aaattccacc aggattcaga
     2221 gggtaacgat gaaaaacgtt ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc
     2281 atatcaaacc tgcattcaaa acgggtgaag agctactggc ctggcagaaa gaacaagggg
     2341 cgatccgctc cgccgctctc gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta
     2401 accgctccgg tattcgtcca ctgcatcaga actgctcctt tgagaactat cgcgttgagt
     2461 gtgaagggca gatgaatgcg ttaagcaaag cgcgccagta tgtcgaagag tttgacggca
     2521 acatcgccag tttcatcttt tctggtaagc caggaaccgg caaaaaccat ctggcggcgg
     2581 caatctgcaa cgagctgctg ctacgcggta aatccgtatt gatcatcacc gtggccgata
     2641 ttatgtcggc gatgaaagat accttcagga atagcggaac cagcgaagaa caactgctta
     2701 acgatctgag caacgttgat ctgctggtga tcgatgagat cggcgtgcag accgaatcga
     2761 aatacgaaaa agtgatcatc aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa
     2821 ccgggatgct gaccaacagt aatatggaag agatgaccaa gttgctgggc gaacgcgtga
     2881 tggaccgtat gcgcctgggt aacagtttgt gggtgatctt caactgggat agctaccgta
     2941 gccgtgtaac aggtaaagag tattaatccc cgtttctggc ggtatactgc gacacaattc
     3001 caccttgagt aatgatgatg aaaactgtta aacatcttct gtgctgtgcc attgccgcca
     3061 gcgcattaat ttccaccggg gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg
     3121 ccagcgaact tggcaaccaa aacagcacga cacaggaagg cggttggtcg ctcgcgtcat
     3181 taactaactt gcttagcagc ggaaaccagg ccttaagcgc agataacatg aacaatgccg
     3241 caggcattct gcaatactgc gcgaagcaaa agctggcgtc ggtaaccgat gccgaaaaca
     3301 tcaagaacca ggtgctggaa aagctgggcc tgaacagtga agagcaaaaa gaagacacca
     3361 actatctgga cggtattcag ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg
     3421 ataacatcgg aacgactccg ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt
     3481 taaagcaggg gctgaacttc atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc
     3541 ggctgacgcg gctctctcct gcccttttcc ctgctcctgg acggttttcc ccctgttaca
     3601 cgggaagcga ccgcgatcaa aaaagtctgt ttataaacta attcctaaca acatcgcatt
     3661 tttttgacac tagaattgca gcaatatggc gacgccatta cattgtgctt ctcaaaaagc
     3721 ttaaacgttg gctaaacggc ccggtttagt gaggatcgcc cgttgtcaga actactctct
     3781 ttcgcccttt ttctcgcctc tgtgctgatt tacgcatgga aagcgggacg taacacctgg
     3841 tggtttgcag ccacgttaac ggtgctgggg ctatttgtcg ttttaaatat caccctgttt
     3901 gccagcgact attttactgg cgatggtatt aacgacgcgg ttctctatac cttaaccaac
     3961 agcctgaccg gtgctggcgt cagcaaatac atcctgccgg gtatcggcat tgtgctgggg
     4021 ctgacggcgg tgttcggtgc gctgggctgg atcctgcgcc gtcgtcgtca tcatccgcac
     4081 cattttggtt atagcctgct ggcgctctta ctggcgctgg gttcggtgga cgccagcccg
     4141 gcatttcgtc agataacgga actggtgaaa tcccagtcac gcgatggcga cccggacttt
     4201 gcggcttatt ataaagagcc gtcgaaaact atcccagacc cgaaactcaa cctggtttat
     4261 atctacggcg aaagtctcga gcggacctat tttgataacg aagctttccc ggatctcacg
     4321 cctgaactgg gcgcgttgaa aaatgaaggc ctggatttca gccacacgca gcagctgcca
     4381 ggaacggatt acacgattgc gggcatggtg gcttctcagt gcggcatacc gctgtttgcc
     4441 ccctttgaag gcaacgcctc cgcttctgtt tccagcttct tcccgcagaa catctgtctg
     4501 ggcgatatcc tgaaaaactc gggttatcag aactatttcg tgcagggcgc aaatctgcgt
     4561 tttgccggta aagatgtgtt cctgaagtcg catggcttcg accacttata cggctcagaa
     4621 gagctgaaaa gcgtggtagc cgacccgcac tatcgcaacg actggggatt ctacgacgat
     4681 accgttctcg atgaagcgtg gaaaaagttt gaagagcttt cccgctcagg tcagcgattc
     4741 tcactgttta ccctgacagt cgatacccat cacccggatg gttttatctc tcgtacctgt
     4801 aaccgcaaaa aatatgattt tgacggtaaa ccgaatcagt cattcagcgc ggtaagttgc
     4861 agccaggaga atatcgcgac gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat
     4921 accgtcatcg tcgtctcttc tgaccattta gcgatgaaca acacggcgtg gaaatacctc
     4981 aataagcagg atcgcaataa cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag
     5041 acgctggcag tgaagcgtaa cacgatggat aacggcgcga cggtgctgga cattctcggt
     5101 ggcgataact atctcggact tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc
     5161 ttcctcaata tcaaagagaa aacattggcg tggaagccag atatcatccg cctgtggaaa
     5221 ttccctaaag agatgaaaga gttcaccatc gaccagcaga aaaacatgat tgccttctcg
     5281 ggtagccatt tccgtttgcc gctgctgttg cgggtttcag acaaacgcgt ggaaccgctg
     5341 ccggaaagcg aatactcagc accgctgcgt ttccagctgg ccgatttcgc tccacgcgac
     5401 aatttcgtct gggttgaccg ttgctacaag atggcacaac tctgggctcc ggaactggca
     5461 ctttccaccg actggtgtgt ctcgcagggg cagcttggcg gtcagcaaat tgttcagcat
     5521 gttgacaaaa caacatggaa gagcaagacg gcatttaaag atacggtcat cgacatggcg
     5581 cgttacaaag gcaatgtcga tatgctgaag attgttgata acgatattcg ctacaaagcc
     5641 gacagtttca tctttaacgt cgccggtgcg ccagaagagg tgaaacagtt tagcgggatt
     5701 tcccgtccgg agtcgtgggg ccgctggtcc aacgcgcagc tgggcgatga agtaaaaatc
     5761 gagtacaagc atccgctgcc aaagaaattt gacctggtga ttaccgccaa agcatacggc
     5821 aataacgcca gccgtcctat tccggtacgc gtaggcaatg aagaacaaac ccttgtgctg
     5881 ggcaatgaag tgaccaccac cacgctgcat ttcgataacc caaccgatgc cgacacactg
     5941 gtaattgtgc cgccggaacc tgtctcaacc aacgaaggga atatcctcgg acactcgccg
     6001 cgtaagctcg ggatcggcat ggtggaaatt aaagtggtag aacgtgaagg gtaattcctg
     6061 actgatgtgt aggtcgcata gggtgcgacc tacacacctt ctccggctta caacacatta
     6121 ttaagaaaca aaaaaaacag cgcccaacac ttccccggac gctgcatggc aactttaata
     6181 atctcgcctt atcagaaagt aattacgcct ttaattaact cacgattgtt aatcacatcg
     6241 cgctcgtagg tttctgccag cgtggcgaac ggatagcgat gggttaacat catgtcagca
     6301 gtgatttttc cttccgccat cagtcgaccg actttagcaa aatcttccgg cgtggcgtta
     6361 cggctaccca tcatcgtcgt ttctttttta tggaattccg gatcggagaa ctgcaactca
     6421 cctttaaaca ggccgacaaa taccaccgtg ccgccgtgac gaatcagatt cacggtgtta
     6481 ttcatcgcat gttgattacc tgtcgcgtcg atcactttct gcgccagcga accaccaaac
     6541 tgcgcccgca gctgcgcgtc aaaatcttcg gctgacgggt ccagcacggg caactccaga
     6601 cgcgttgcca catgttcacg gcgcgccgga ctggtatccg ccaccaccac ctgtgcgcca
     6661 tcggctttag cgattgccgc cgcgcccaga ccgattggcc ccgccccgac caccagcacc
     6721 tgctcgccgg gagcaatggc tgcgcgacgc accgcatgag cgctaatagc gaaaggttca
     6781 atcaatgcgg ccgcctgcgg gtcaataccg tctgcgggca aaatgttcgc caccggcacg
     6841 ctcaaatact cactaaaacc gccatcctga tgcacgccaa tgactgaaat tttttcacag
     6901 caattggtac gcccgctttt acacgccggg cattgctgac aggcaacata agggatcaca
     6961 gcaacttgct gaccattttt aagatcagca atatttttac ccagcccaac aatctcccca
     7021 catatttcat ggcctaaaac ccgtggataa ctaaaaaatg gttgatttcc accccaggca
     7081 tgaatatcgg taccgcaaat cccgacagac ttaattttta ttaatgcttc attgtcaccc
     7141 ggaataggta tctcgcgttg tttccagact aattctttcg gctgctggca aattaaaaca
     7201 ttcatcgtag acatgattcg gctctccatt ttttgttgcc ttagatatca atgaaaatga
     7261 aaaatggcag cggaacggaa aatctgtttt gtgaaaacac acgcataaaa atgttttaaa
     7321 tcgggtttta atggactcac aaggaggaaa atatgagtcg ttcacaaaat ttacgccaca
     7381 atgtgattaa ccaggtcatt gatgatatgg cgcggggtca tatcccttcc ccacttccat
     7441 cgcaaagtgc gctggcggaa atgtacaaca tcagccgcac tacagtgcgt catattctca
     7501 gccacttacg cgaatgcggc gtcttaacgc aggttggaaa cgactatgtt attgcgcgta
     7561 aacctgacca tgatgacggt ttcgcctgac taccgcttca atgagtgaac aaaacaaagt
     7621 gtttgagcag gcttttttca cgatgataaa ccagcgtcag ttacgccctg gggaaacttt
     7681 ttctgaactg caactggctc gggccgcggg tgtcagcccg gtggttgtaa gagaatatct
     7741 tttaaaattc ggccgttata atcttattca aagcgaaaag cgcggccagt ggagcatgaa
     7801 gcaattcgat caatcctacg ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca
     7861 ttcacttcag cactttctca acctgcctga tcatgatccc cgctggctgc aggcgaaaac
     7921 catgctagaa cgtcatcggc tactgcgtga caacatcggc aatagctttc gcatgttctc
     7981 gcaacttgac agagattttc actccttact gctttcagct gccgacaata tcttttttga
     8041 tcaatcgctt gagatcatct cagtcatctt tcattttcac tatcaatggg acgaaagcga
     8101 tcttaaacag cgcaatatca ttgccgtcga tgagcatatg accattctca gcgctctgat
     8161 ttgccgcagt gaccttgatg ccacccttgc gctccgtaac catttgaatt cagctaaaca
     8221 atcaatgatt cgctccatta atgagaacac gcgttatgct cattaagtac ggattaaaaa
     8281 cagcaaacta ttagcgggtg cacggtttta tcaaaagata aataccggga aattcccgct
     8341 tacctatgct cacaatcagt cgactaaaaa tgtttttgtg agcggtagta aagtcctaaa
     8401 actttaacct gccgttaatg tcaaccttgc gtaatgcaca attacgtaaa caattaaaat
     8461 tataaataca ggagtccggc gtggaaaaag aaaatatcac cctcgatccg cgttcttcat
     8521 ttactccatc ttcgtcggca gatattcccg tgccaccaga tggattagtt caacgcagta
     8581 cccgaattaa acgcattcaa accaccgcca tgttgttatt attttttgcg gcggtaatca
     8641 attatctcga ccgcagttcg ctgtcggtag caaatttaac gattcgtgaa gaattgggat
     8701 taagtgccac cgaaatcggc gctttgctct ccgtgttttc actcgcttac ggcattgcgc
     8761 aacttccttg cggcccacta ttggatcgta aaggcccacg cctgatgctg ggactgggga
     8821 tgttcttctg gtcactgttc caggccatgt ctggcatggt gcacagcttt acgcagttcg
     8881 tgttggtgcg tatcggtatg gggattggtg aagcgccgat gaacccatgc ggtgtaaaag
     8941 tcattaacga ctggttcaac atcaaagagc gcggacgccc gatgggcttc ttcaacgcag
     9001 cttctaccat tggcgttgcc gtaagcccac cgattctggc ggcgatgatg ctggtgatgg
     9061 gctggcgcgg gatgtttatt accattggtg tactggggat ttttctcgcc atcggctggt
     9121 atatgctcta tcgcaaccgc gagcacgtag aactgactgc cgttgaacaa gcttatctca
     9181 atgcaggtag cgtcaatgtc cgccgagatc cgctcagttt tgccgaatgg cgcagcctgt
     9241 tccgtaaccg cacaatgtgg ggaatgatgc tcggattcag tggcatcaac tacactgcgt
     9301 ggctgtatct ggcctggctt cctggttacc tgcaaacagc ctataacctg gatttaaaaa
     9361 gcacagggtt gatggcggct atccctttcc tgtttggggc tgccgggatg ctggtcaacg
     9421 gttacgttac tgactggctg gtcaaagggg gaatggctcc gattaaaagc cgtaagatct
     9481 gcattattgc cgggatgttc tgttctgccg cctttacgct gatagtacca caagcgacaa
     9541 catccatgat ggcggttctg ctgattggca tggcactgtt ctgtattcac tttgccggaa
     9601 catcctgctg gggcttgatc cacgtcgcag ttgcttctcg catgactgcg tcggtgggca
     9661 gtatccagaa ctttgccagc ttcatctgcg cctctttcgc gccgatcatt actggtttta
     9721 ttgttgatac aacccactca ttccgtctgg cactaatcat ttgcggttgc gtcaccgcag
     9781 cgggagcact ggcgtacatc ttcctggttc gtcagccgat caacgatccc gcgtaaagat
     9841 taaactctct atgcccgata acattttgct tatcgggcat ttttatggcg attaaaacgt
     9901 ttcccagttc tccccgctat ctgccactgc cattttacgc ggcgtagttg gcgtcacggt
     9961 ttttaccaca gccgatgttt cacgctgctg ttgctgctgt tgctgaatcc ggaacactgc
    10021 cacagcttcg gtcaggcgac tggcctgctc ttccagcgcg gcggcggcag cggcagactc
    10081 ttccaccagc gcggcgttct gttgagttac ccggtccatc tcagcaaccg ctaagccaac
    10141 ctgatcgata ccacggctct gctcatcaga agcagaagct atttcgccca taatgtccgt
    10201 cacgcgggtc acggcactga caatctccgc cattgtttcc ccggcgcttt cgaccagcgt
    10261 agagccaaca tccactttcc ccaccgagtc ttcaatcagg cttttaattt cacgagccgc
    10321 ctgggcgcta cgctgggcca gattacgcac ttctcccgca accaccgcaa aaccgcgtcc
    10381 ttgctcaccc gcacgcgctg cttcaaccgc cgcgttcaaa gccagaatat tggtctggaa
    10441 ggcaatgccg tcaattacac tgataatatc ggcgattttc tgcgaactgg tggagatatc
    10501 gcgcatagtc tgcaccacgt tatctaccac tttgccgccg cgttgcgccg tttcggaagc
    10561 acttaacgcc agatggctgg cctggcgcgc attctcggcg ttctgtttca ccgttgcggt
    10621 cagttgctcc atgctggctg ccgtctcttc cagcgaagcg gcctgttgct cggtgcgcga
    10681 agagagatcg ttattgccgg tggcgatttc gctggcaccg ctatagatgg cattggcccc
    10741 gttgcgcaca tcaccgacgg tacgcatcag ctctccctgc atatggcgca aactctctgc
    10801 cagttgcccc atctcattag agccatccac ctcaatcggt ttcaccagat cgccgcctgc
    10861 aatatgacga atgctgtcaa tcaggcgatt cattggcgct accagcgagg ctttaatacc
    10921 gaaccagacg gcgaagatga ccgccagtac gacgatcatc acgcccacca gaatccacat
    10981 cgcctggctg taggaggcat tgttatcgct gacggcgata tcatagagcc gatcgttttg
    11041 ctccatgtaa gccacatact gcttctcgaa accgtcctga tatccctggg tcggctgatc
    11101 aaagaactcg ttgattttgc ctgcacctaa tagttggatc agctccgcca gcgcattgtg
    11161 ataaatatcg taattacgtt tgatctctgc cgctgcggcg gtgctctgac gcgggtcacg
    11221 cggcaacgct tcgtaatctg cccagttttt ttccgcctgt ttcagcgaaa tactggcact
    11281 ctgcatcagc tcagcaacgg ttgaaccgct accaatatta ttctgatcca tcatgtagcg
    11341 gatacccgcg cggttgaggg tgttacgcgt ctgcaacaac gcgacccagc tgccattcag
    11401 cgtggattgc tgctggcgaa tggtttgtaa aacagtgaaa ttttctttgt cattctttaa
    11461 ggcattaaag aacagaccgc ctgatgtcag ttgtaaaagg ccaaaaacgg ccaaaaccag
    11521 cagtaagctg gtcacgatct tgatacgttt taacatggtt tctctttcct gtggaccgat
    11581 gcaagatttt cggcctggaa agcaaaaact ttatgaaaag gtgtaacccg ccatccatgg
    11641 aaaaaccagg cgctggttat cagatactaa aaagttattc attatcgccc gggatgtctt
    11701 cttgtcggga attgcccaga gcaataaatt cctctaacag atgggttaac tgttgcattt
    11761 tctcggcagt aaactgagct tcaatctgcc gataggcttc ttcaatctgt gtctgagcgc
    11821 ggttatacag cgcctgcccc tctttggtca gcgagatata gagcttccgc tgatcattga
    11881 tcggctttaa tcgcaacact aaaccgtcgc gttccatccg cgtgaggatc ccggtcaggc
    11941 ttgggcgcaa aatgcaggcg cgatacgcca gatcgtgaaa atccattgat gggctttccg
    12001 ccagaatacg cacgatgcgc cactgttgct cagtcaaatt atgccgttta acgattgggc
    12061 gaaaataact catcgccgct tctcgcgcct ggagcaacgc aatggttagt gagtcgtgca
    12121 ttatctttcc cctgaaaaat tcaaaatcat taatatagaa acaattaatc atcacgtta
//
LOCUS       DAPHJE010000125        11649 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPHJE010000125 DAPHJE010000000
VERSION     DAPHJE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.126"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            247..1635
                     /gene="narU"
                     /locus_tag="RH222_003682"
     CDS             247..1635
                     /gene="narU"
                     /locus_tag="RH222_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV3798636.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1717..5457
                     /gene="narZ"
                     /locus_tag="RH222_003683"
     CDS             1717..5457
                     /gene="narZ"
                     /locus_tag="RH222_003683"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV3798637.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5454..6998
                     /gene="narH"
                     /locus_tag="RH222_003684"
     CDS             5454..6998
                     /gene="narH"
                     /locus_tag="RH222_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV3798638.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6998..7693
                     /gene="narW"
                     /locus_tag="RH222_003685"
     CDS             6998..7693
                     /gene="narW"
                     /locus_tag="RH222_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV3798639.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7690..8370
                     /gene="narI"
                     /locus_tag="RH222_003686"
     CDS             7690..8370
                     /gene="narI"
                     /locus_tag="RH222_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV3798640.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8449..9342
                     /locus_tag="RH222_003687"
     CDS             8449..9342
                     /locus_tag="RH222_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV3798641.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9438..10283)
                     /gene="nhoA"
                     /locus_tag="RH222_003688"
     CDS             complement(9438..10283)
                     /gene="nhoA"
                     /locus_tag="RH222_003688"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV3798642.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIAHRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10456..11025
                     /locus_tag="RH222_003689"
     CDS             10456..11025
                     /locus_tag="RH222_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV3798643.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11029..11256)
                     /gene="pptA"
                     /locus_tag="RH222_003690"
     CDS             complement(11029..11256)
                     /gene="pptA"
                     /locus_tag="RH222_003690"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV3798644.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11307..11649)
                     /locus_tag="RH222_003691"
                     /pseudo
     CDS             complement(11307..11649)
                     /locus_tag="RH222_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2733 a         3032 c         3050 g         2834 t
ORIGIN      
        1 atctgaaggg ttgaaacatg acatacgttc aaagattaac ccccgtttga aggttagctt
       61 gttagttaca tttagtaaca catatctggc tacttaatcc ccattctgcc cccgcggtta
      121 cgctttctta tctattcttt caatcagcac ttcacccaat tcatccttaa ttctaataac
      181 tggctattgc gccttacctc ctgttttgtc ttttttcatt accaatgtgt gcatgtgagg
      241 aacaatatgg cactgcaaaa tgagaaaaat agtcgttatc ttttacgcga ctggaaacca
      301 gaaaatcctg ccttctggga aaataaagga aaacatattg ctcgaagaaa cctctggata
      361 tcagtcagtt gtctacttct tgccttctgt gtctggatgc tatttagcgc agttaccgtt
      421 aatctcaata aaatcggttt taattttact accgatcaac tctttttatt aaccgcatta
      481 ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc tatattcggt
      541 ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt ctggctcgga
      601 attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc tttgctatgc
      661 ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt ctttccaaaa
      721 gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg tgtaagtgta
      781 atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct cggcgtcaat
      841 ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg gatttgggta
      901 ccgctactgg cgattgccac gatcgctgcc tggtcaggga tgaatgatat cgccagttca
      961 cgcgcctcaa ttgccgacca gctccctgtc ttacaacgcc tgcatctctg gctgctgagc
     1021 ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt tgccatgctg
     1081 gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg cccatttatc
     1141 ggtgccatcg cgcggtcggt tggtggtgct atttccgata agttcggcgg cgtgcgggtg
     1201 acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct taccttaccg
     1261 ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct gtttctgact
     1321 gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg ccagataacc
     1381 atttatcggg taaagatgaa aggcggtagt gatgagcaag ctcagaaaga agccgtcacc
     1441 gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg cttttttatt
     1501 ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc gatgaaagtg
     1561 tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg tcggcggaag
     1621 ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc gacaaggcat
     1681 cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt ggatcgcttt
     1741 cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt gatgcatagc
     1801 aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat cgtgcgttcc
     1861 acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa aaatggtctg
     1921 gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacct gcccaatcat
     1981 gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctttacag cgctaaccgc
     2041 ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgcgaagc cctcaagcaa
     2101 cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa gtgcctgagc
     2161 tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga actaaaccag
     2221 ctgattgccg ccgctaacgt ctggaccatc aaaacctacg gcccggatcg cgttgccggt
     2281 ttctcgccga tcccggcgat gtcgatggtt tcttacgccg ccggaacgcg ttatctgtcg
     2341 ctgcttggcg gcacctgttt aagtttctac gactggtatt gcgacctgcc gcccgcctcg
     2401 ccgatgacct ggggcgagca aaccgacgta ccggaatctg ccgactggta taactccagc
     2461 tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc ccacttcttt
     2521 accgaagtac gctacaaagg cactaaaacc atcgccatta cccctgacta ctctgaagtg
     2581 gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc cctggcgatg
     2641 gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga ctactttatc
     2701 aactactgcc gccgctacag cgacatgccg atgctggtaa tgctggagcc tcgcgacgat
     2761 ggtagctacg ttcccgggcg catgatccgc gcatctgacc tggtggatgg actgggcgaa
     2821 agcaacaatc cgcagtggaa aaccgtagca gttaataccg caggtgaatt ggtagtgccg
     2881 aacggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga atccattgcc
     2941 gccggtacgg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc tgttgcaggc
     3001 gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt aaaacacaat
     3061 ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg caacacctgt
     3121 ccggtggtca gcgtttatga tttggtactg gcgaattacg gcctcgatcg cgggctggaa
     3181 gatgaaaaca gtgcgaaaga ttacgctgaa atcaaaccgt acaccccagc ctggggtgag
     3241 caaattaccg gcgtgccgcg ccagtatatt gaaaccatcg cccgtgaatt tgccgatacc
     3301 gcccataaaa cgcatgggcg ctcgatgatt atcctcggcg caggtgttaa ccactggtat
     3361 cacatggaca tgaactaccg cgggatgatc aatatgctga tcttctgcgg ttgtgtcggg
     3421 caaagcggtg gcggctgggc acactatgtc ggccaggaaa aactgcgccc acaaaccggc
     3481 tggttgccac tggcctttgc gctcgactgg aaccgaccac cgcgccaaat gaacagcacc
     3541 tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc tcaggagtta
     3601 ctttcaccgc tcgccgatgc cagtaagtac agcggtcatc tgattgattt caacgttcgc
     3661 gccgaacgta tgggctggct accttctgcg ccgcagctgg ggcgtaaccc gctcgggatt
     3721 aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca ggcgctgaaa
     3781 tcgggcgatt tacgtatggc ctgcgaacaa ccagatagca gcagcaatca tccgcgtaat
     3841 ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga gtatatgcag
     3901 aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc cagcgacggg
     3961 atcaaaccgg aagaagtcga gtggcaaact gcagcgattg aaggcaagct cgacctgctg
     4021 gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt tctgcccacc
     4081 gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt tattcatccg
     4141 ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat ctacaaaggt
     4201 atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac cgacgtggta
     4261 ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga agtgctcgac
     4321 tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat tgtggcggtg
     4381 gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt gatggacaaa
     4441 cttggcaatg gcggtaaagg gatttcgtgg aatacgcagg atgaaatcga tttcctcggc
     4501 aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat tgacaccgcc
     4561 attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca tgttgcagtc
     4621 aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct ggcgctgcac
     4681 aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa aattatctcc
     4741 agccccacct ggtccggtct ggaaagcgat cacgtatcct ataacgcggg atacaccaac
     4801 gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta tcaggatcat
     4861 ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat cgacacccgt
     4921 agcgtcagtg agatgcgcca gataccgcca aacggcttcc cggaaaaagc acttaacttc
     4981 ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct gctaatgctg
     5041 acgctctctc gcggtggacc gattgtctgg atcagcgaaa cagatgcccg agaactgacc
     5101 attgtcgata acgactgggt ggaagtattc aacgccaacg gcgcgctgac tgcccgcgcg
     5161 gtggtcagcc aacgtgtacc gccgggcatg accatgatgt atcacgccca ggaacgcatt
     5221 atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa ctcggtcacc
     5281 cgcgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc ctggggcttt
     5341 aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg caaaatgaaa
     5401 aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa aaaatgaaaa
     5461 tacgttcgca agtcggcatg gtgcttaacc tcgataaatg tatcggctgc catacctgtt
     5521 cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca tggtttaaca
     5581 acgtcgaaac caaaccgggc attggttatc ctaaaaactg ggaagatcag gaagagtggc
     5641 aaggcggctg ggtccgcgat gtgaatggca agatccgtcc gcgtctgggc agcaagatgg
     5701 gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat tactacgaac
     5761 ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat atccctactg
     5821 ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg ggcccaaact
     5881 gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac ttcgaggcca
     5941 tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg ccgcgcctgt
     6001 gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct atctacaaac
     6061 gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg cgtttgtgca
     6121 ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag tcagaaaaat
     6181 gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca gaaacctgcg
     6241 tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt gaggaagcgg
     6301 cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc ctcgatccac
     6361 acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac gtgattgaag
     6421 ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg ctaccgctgc
     6481 accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg ccgattcagt
     6541 cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc atcgaaagcc
     6601 tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc ggtccggtac
     6661 tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa accgtggaag
     6721 gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag gtcgaagaaa
     6781 tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg accagccatc
     6841 gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc tttggcgacg
     6901 gttgtcacgg ctcggacagc aaattcaacc tgttcaacag tagccgtatc gatgccatca
     6961 acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca aagtgatcgg
     7021 cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg cgctggcgtt
     7081 gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta acgcgccgct
     7141 gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca cgtcgctgct
     7201 gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg tggacctgct
     7261 ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg attatctacc
     7321 gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag gattgctcaa
     7381 tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg caccgtggta
     7441 cgcgttgttt gatgctctgc tgcaactggc gggaagctcc ctttcaagtg acagtgtcac
     7501 aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg cagtgtggga
     7561 agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct cgccgcttaa
     7621 tcaatatcag cgacgcttta gccaggatgt cgcgccgcaa tatgtcgaca tcagtgcggg
     7681 aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc gtacatttgt
     7741 gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta cacctggcgc
     7801 gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa tctgttccat
     7861 atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc gcactggatg
     7921 tacgcgtggt ttttaccagt ggcagcgaaa caactcatgg cgatggtgct cggcggtatt
     7981 tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcaggct gacaaatcag
     8041 cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt gctgatccag
     8101 tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg tagcgaaatg
     8161 atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc gtcagagatg
     8221 ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac catcttcctg
     8281 ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta ctttactcgt
     8341 cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc ccttcgctct
     8401 ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat gaaaccgcag
     8461 gtgtaccacg tcgatgcttt tacctcacaa ccgtttcgcg gcaattctgc cggtgttgtt
     8521 ttccccgccg ataatcttag cgaagcgcaa atgcagctta tcgcccgcga gttaggccat
     8581 tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg ctactttacg
     8641 ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta tgtacgtgcg
     8701 aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg aaaacatcgc
     8761 gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg tacgccgggc
     8821 tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt gcatcttact
     8881 gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca ttctaaagtg
     8941 atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct taatgcgctg
     9001 accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat ccgcccaggc
     9061 aaaaacgaaa ccgatggtcg catgttctcg cctgcgattg gtattgtgga agatccggtg
     9121 actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt attgccccac
     9181 gatggcaagg tgctgcgtgt taaaggccat caggggcgcg cattggggcg tgacggcgtg
     9241 attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat ttctggcgcg
     9301 gcggtgattt tgtttcatgc cgaatgggca attaaactct gaaaccgttg cctgatgcgc
     9361 ttcgcttatc aggtctacaa ccgagctaat gtatgccgga taaggcgttt acgccgcatc
     9421 cggcaacctg acataaatta ttttcccgcc tccgggtgcg tatcaaaagc cgccatcacc
     9481 agcgctaact catccacggt aaagccatgt ttcgcatcat ccacgcccag accaaactgt
     9541 tcttgcatca cagcatataa tgacgccaca tccggtagat ttcgctgctc caccgcgtgc
     9601 ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc gtccggcaaa
     9661 tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc cgaccagaaa
     9721 ttgcccatca cataatcgct ttgttgctgc tcgcagagat caaaacggta catcgactgc
     9781 cagtgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa caaccgataa
     9841 tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc ggttagcgtc
     9901 tgcccaccga aaccgacatc agcaatccat ttttcctctt ccagttccac caacagcaaa
     9961 cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc taacaagctg
    10021 cgaacgttaa actccagctc gcgtaacacc cgctcaaaca cgccattctg ctcaaaacag
    10081 taaccgccac gatgggctat caccagtttc tcttccagcg attgatcatc aagctgtatt
    10141 tccctcggca gcaaaacgtc gaggttttca aacggaatag tgcaattgtg tttcaggtgc
    10201 aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat acgggcaaaa
    10261 tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca acttataacc
    10321 tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac gtttgcaaat
    10381 cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa aaaaccttct
    10441 tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc cagccgtctg
    10501 ttaaatcatg gcccaactgt catgatcacc agtttcgatg agcaatccca gcggcgtaac
    10561 attatggcag cagcctggtc aatgccggtg gagtttgaac cgccacgcgt ggcgattgtg
    10621 gtagataaat cgacatggac cagggagttg attgaacgta acggtaaatt tggcatcgtt
    10681 atcccgggcg ttgcagcaac taactggacg tgggcagtgg gaagcgtgtc gggtcgtgaa
    10741 gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct tggtttgcca
    10801 cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc gacttctgcg
    10861 caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga cgcacgggta
    10921 tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca tcatttaggt
    10981 gctggcacgt ttgttaccag cggcaagcga gttacggcgg gttaagcatt acatgctgta
    11041 accaggtttc tttatcaaag cctccatttg gggcgcgatt tcggcatccc agatagcttg
    11101 ccaagattct ggttgaatct gctgtagagc aatgcttatc gaactgtctt tactgttcag
    11161 atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt gttcgtccag
    11221 ttcacgcgga aaacatttaa tgtcgatgtg cggcatgatt gtgctccttt tgaaagatgt
    11281 cagtaaaatt aacacacatt gtgaattttc agtcctttcc attcaaacgt gaaatcaata
    11341 agttcatcag ggacatcgca aacaatatga atacggattt cttctctgcc gtgactcttt
    11401 tcacaaattg cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc
    11461 tcaaaggctt tatttagcca cccctggttt cctttcaccg cgaataaata atcacctccc
    11521 tgtttttgta tcttctctgc tatatctttc tggcaaccca tcgcatcagt tgtgatgatt
    11581 tttcgtttaa tatccagcat gttaagaagt tctgggatag ctgtaatctc attagattta
    11641 tcatccgtc
//
LOCUS       DAPHJE010000126        11345 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPHJE010000126 DAPHJE010000000
VERSION     DAPHJE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.127"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..243)
                     /locus_tag="RH222_003692"
     CDS             complement(<1..243)
                     /locus_tag="RH222_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798645.1"
                     /translation="MVKPNWDNFKAKFSENPQGNFEWFCYLLFCQEFKMPAGIFRYKN
                     QSGIETNPITKDNEIIGWQSKFYDTKLSDNKADLIEM"
     gene            complement(319..738)
                     /locus_tag="RH222_003693"
     CDS             complement(319..738)
                     /locus_tag="RH222_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798646.1"
                     /translation="MAKHLDNRDINAIVNLIRGWKEPKLSWSAICEAAEPLVGKLPSR
                     QSLNAHEAIVTAYQTKKEALKGRGPKNPRPASLNIAAARIVNLESEIEELKEENRRYK
                     QQFVIWQYNAYKYGMTEHQLNAQLTKIDRERSDGERR"
     gene            complement(731..2764)
                     /locus_tag="RH222_003694"
     CDS             complement(731..2764)
                     /locus_tag="RH222_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3798647.1"
                     /translation="MAEIFLFKPKATLTAAENLEAFISQCRDQLTVFGSDLTWEDPVW
                     PNITVFAKLGIITRKPILEETQDPAFIDFAKAYFRYQQGHSLSRAKNESKALRAVEAA
                     LLQVNGNANIDGLSISVLDEAAELAKHYYSDGSAYHCGREIERLAKFVAENQLVSSAV
                     QNWVNPIKRAEDKNKTSRKAKKNREDKLPSDIALNALAEIFANDPIHERDIFTTSVFA
                     MLMSAPSRITEVLALPVDCEVFETDREGIERYGWRFFAGKGYEGDIKLIPTVMVGIAK
                     TAVARARVLSENARQLAKWIEKHPNKFYRHANCPSVADDEPLTMKQVCMALGFAHYSK
                     KTCRSCLGNRGLAKEDGVHTLNSLWQHTMARLPDGFPWFDKDKGIRYSNALFVLNVNQ
                     FHGNRGCIPVELHKPTNNFFNSDLTPRLSLKGKHASIFDRHGYHAVNGEPVKLTSHQA
                     RHLLNTIIQRGGLSNLEIAKWSGRADVKQNRTYNHMTEYELVGIAERLDPTTALFGPA
                     GEVAKHLPVTMQEFNTLEHAAVHVTEYGYCVHDYTMSPCEKFRDCVNCTEQVCIKGDD
                     AEKLDRIKIRLEKLERLFFLADAAVESGEIGTDRWYQYHKKTVTRLRELVAILENPDI
                     ENGVQIKLRGNDFSQLRRVVAKTSIVAIEQKGKESEEAVMLDDLTTLLGGGLG"
     gene            complement(2781..4319)
                     /locus_tag="RH222_003695"
     CDS             complement(2781..4319)
                     /locus_tag="RH222_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDV3798648.1"
                     /translation="MTMNNLLLGLVPERIRARANGYSFLLYDTSWTLDRNITINLADI
                     HHYLSDGTLDGCLRTLAFYASNRSSSHTKNILERFQHMLRETDAREITDTVLINYRSM
                     LSPATEWYLATIRGFLRSWHELGYPGISKEVNRLLDGWTLKGNRKGDAVKRMDPQEGP
                     LTDNELTAFNEGAVRAYEKSLITIAELAIALGTSHTGRRPIQISLLRVVDILCGKNGK
                     GEPFYVLNVPRAKQRSSSFRINFKPFAISLELWAILSAQARNAIAQVENRLGFELQED
                     DRQQIPLFPDLDVLDAVQSPYEFRQLHATDKLHIPAAEITDTLQYIVEKSDIRSERTG
                     ELLHINARRFRYTTGTRAAREGFGELVIAELLDHADTQNAGVYIKNIPEHVKKLDEAV
                     GFQLAPYAQAFVGALVDSERDAHRGNDPTSRIRTEMGQGVGTCGEHGFCGANVPIPCY
                     TCMHFQPWLDGPHEDVYQGLLNERERVKEITGDIQIAAVLDRSIIALADVIMRCAKRR
                     EELS"
     gene            complement(4306..5553)
                     /locus_tag="RH222_003696"
     CDS             complement(4306..5553)
                     /locus_tag="RH222_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3798649.1"
                     /translation="MEKNYRVTTFVMDSGERYCMVVDRASGLPMYYPTLFLTTQIRNR
                     EDAFSTMLAAANNLVMLLRFVESRDIDLEQRFLTKDFFKPHELDDLRDFAQCKQRKGP
                     LTVSSTSWLADAMTDIVDNGTQHSRLTTFANYLHWYAMHILKTTELEVVEKINAMAAQ
                     IKTRRPSKKRRNHDLQDRSLSDVQLDALFEVIQPRSELNPFSIDVQRRNRLMILLLFY
                     LGIRGGELLNIRIQDIDFSANRIRIVRRADEQADSRTNEPNAKTRERLLPLAESLAQE
                     LHSYILQDRRKVRNAKKNDYLFVTYKSGPTLGNPISKGGYHKIFSVVRSISPQLYAAT
                     GHSLRHTWNRKFSERMDAMNEQVSEERQEQLRSYLMGWRDGSGTAATYNKRFIRQKGF
                     EAALALQAGNGTSLPEDFKDDHE"
     gene            complement(5712..6248)
                     /gene="ssb1"
                     /locus_tag="RH222_003697"
     CDS             complement(5712..6248)
                     /gene="ssb1"
                     /locus_tag="RH222_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV3798650.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            6502..9324
                     /gene="uvrA"
                     /locus_tag="RH222_003698"
     CDS             6502..9324
                     /gene="uvrA"
                     /locus_tag="RH222_003698"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV3798651.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(9359..9715)
                     /locus_tag="RH222_003699"
     CDS             complement(9359..9715)
                     /locus_tag="RH222_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV3798652.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(9719..10135)
                     /locus_tag="RH222_003700"
     CDS             complement(9719..10135)
                     /locus_tag="RH222_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV3798653.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(10246..10959)
                     /gene="aphA"
                     /locus_tag="RH222_003701"
     CDS             complement(10246..10959)
                     /gene="aphA"
                     /locus_tag="RH222_003701"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV3798654.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVDARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2797 a         2890 c         2668 g         2990 t
ORIGIN      
        1 catttctata agatcagctt tgttatccga caattttgtg tcatagaatt tagattgcca
       61 accgataatt tcattatctt tggttattgg attagtttcg ataccagatt gattcttata
      121 tctaaatata cctgcgggca ttttgaattc ttgacagaac aacaagtagc aaaaccactc
      181 aaaattacct tgaggattct cactaaattt agctttaaag ttatcccagt ttggtttgac
      241 catttaatta ttctcctatt tttttgtaaa taactacccc tacacagaga aatgtaccct
      301 cgatatcgta ctcagcaact atcttctttc tccatcagag cgctcacggt caatctttgt
      361 cagctgcgca ttaagttgat gttctgtcat tccatatttg tatgcattgt attgccagat
      421 cacaaactgt tgtttgtacc tccggttctc ttccttaagc tcttcgattt cgctttcaag
      481 attcacaatc ctcgcggctg caatgttcaa actagctgga cgcggatttt tggggccacg
      541 gcctttaagc gcctctttct ttgtttgata ggcagtcact attgcctcat gtgcattaag
      601 ggactgtctt gacggcaact ttccgaccaa tggttctgcc gcctcacaaa ttgctgacca
      661 ggacagtttt ggttccttcc atcctcgaat tagatttacg atggcgttaa tgtctctatt
      721 gtccagatgc ttagccaagg ccacctccta acaaagttgt caaatcatct aacatcacag
      781 cttcttcact ttctttgcct ttttgctcga tggccacaat agacgtcttt gcgacaacac
      841 ggcgaagctg gctgaaatca ttgccccgga gcttaatctg gacaccattt tcaatgtctg
      901 gattctctaa aatagccact agttcacgaa gccgggtaac agttttcttg tggtactgat
      961 accagcgatc agttcctatc tcaccacttt ccactgctgc gtcagcaaga aaaaacagtc
     1021 gctctaattt ctccagacgt atcttaatgc gatccaactt ttcagcatca tcgcctttga
     1081 tgcaaacctg ttcggtacaa ttgacgcaat ctcggaattt ctcacaagga ctcatggtgt
     1141 aatcatgcac acagtagcca tactccgtca catggactgc ggcatgctct aaggtattga
     1201 actcctgcat agtcacgggc agatgctttg ccacctcgcc agcaggccca aatagcgctg
     1261 ttgtgggatc aaggcgttca gcaatgccaa ccagttcgta ttcagtcatg tggttgtaag
     1321 ttcgattctg tttcacatct gcccgccccg accacttggc aatttccaag tttgacaggc
     1381 caccgcgctg gataatggtg ttgagcaggt gtcgagcctg atgtgaggtt aatttcaccg
     1441 gctctccgtt tacagcatgg taaccatggc gatcaaagat actggcatgt tttcctttca
     1501 aggacagacg aggcgtcaga tcactgttga agaaattatt ggttggctta tgtaactcaa
     1561 ctggtatgca tcctctgttc ccgtggaact gatttacgtt cagtacaaac aaggcattgc
     1621 tgtacctaat tcccttgtct ttatcaaacc acggaaagcc atccggtaaa cgcgccattg
     1681 tgtgctgcca caggctattt agagtatgta caccatcctc tttagctaaa cctcgattac
     1741 caagacacga cctgcacgtc tttttagagt aatgtgcaaa accgagcgcc atacaaacct
     1801 gcttcatcgt caatggctca tcatcggcta cactgggaca gttagcatgg cggtaaaact
     1861 tattcgggtg tttctcgatc cacttggcca gttgtcgtgc attttcagag agcactctag
     1921 ctctagctac ggctgtctta gctataccga ccatcacggt gggtatcaat ttgatatcac
     1981 cctcatagcc tttccccgca aaaaaacgcc aaccatatcg ttctatccct tcacgatccg
     2041 tttcaaaaac ctcgcaatcc acaggcagtg ctaacacctc agtaatacgt gaaggtgccg
     2101 acatcaacat ggcaaaaacc gatgttgtaa atatgtctcg ctcatgaatt gggtcgttgg
     2161 caaaaatttc cgccaacgca ttaagagcaa tatcactcgg aagtttatct tccctatttt
     2221 tcttcgcctt tctgctcgtt ttgttcttat cctctgcacg tttaattgga ttgacccaat
     2281 tttgtacagc acttgataca agctggtttt cagctacaaa cttagccagc cgctcaatct
     2341 cacggccaca atgatatgcg gagccatcgg agtagtaatg ctttgccaat tcagctgctt
     2401 catccagcac agaaatcgac aggccatcaa tatttgcgtt tccatttact tgaagcaaag
     2461 ccgcctcaac cgcacggagg gctttacttt cattctttgc cctagagagg ctatgcccct
     2521 gctggtaacg gaaataggct ttggcaaaat caatgaaagc agggtcttgc gtctcttcca
     2581 gtatgggttt tctcgtaata atgcccaact tggcaaatac agttatgttt ggccaaacag
     2641 gatcttccca agtgagatca gagccgaata cagtcaattg atctcggcac tgggatatga
     2701 atgcttcaag gttttctgct gctgtcagcg tagcctttgg cttaaacagg aaaatctcag
     2761 ccattggtta ttgctccctg ttaactaagc tcttcacgac gttttgcaca acgcatgatc
     2821 acatctgcca gcgcgataat actgcgatca agtacggcag caatctggat gtcgccagtt
     2881 atctctttca cccgttcacg ttcgttgagc agtccttgat agacatcttc atgtgggccg
     2941 tccagccaag gctggaaatg catgcaggtg tagcaaggaa taggaacatt ggcaccacaa
     3001 aagccatgtt ctccacaagt accaacaccc tgacccattt cggtccgaat acgactggta
     3061 gggtcgttac ctcgatgcgc gtctctctct gaatcgacaa gtgccccgac aaaggcctga
     3121 gcgtatggtg ctaattggaa gccaaccgcc tcatcaagct ttttcacatg ctcggggata
     3181 ttcttgatgt aaacgcctgc gttttgggtg tccgcgtggt caagcagttc cgcaatcacc
     3241 agctcgccaa agccttcccg ggcagctcgt gtgcctgtgg tataacgaaa acgacgagca
     3301 ttgatatgta atagctcacc ggtacgttcc gaccggatgt cggacttttc tacgatgtat
     3361 tggagggtgt cggttatctc tgcggcggga atgtggagtt tgtcggtagc atgcagttgg
     3421 cggaattcat aaggagattg gacggcatct aacacatcaa gatcaggaaa aagagggatt
     3481 tgctgccggt catcttcctg caattcaaac cccaaacgat tttcaacctg cgcaatcgca
     3541 tttcttgcct gagcactcag gattgcccat agttccagtg atattgcaaa tggtttgaag
     3601 tttattcgga aagagctgct gcgctgcttg gctcgcggca cattcaacac atagaagggc
     3661 tctccctttc catttttacc acaaaggata tcgaccacac gaagcagcga gatctgaatg
     3721 ggacgacgtc ctgtatgact ggtgccaagt gctatagcca actcagctat agtaattaag
     3781 ctcttttcat aagcccgaac agccccctcg ttaaacgctg tcagctcatt atccgtcagt
     3841 ggcccctctt gaggatccat gcgcttaacc gcatccccct tacgattacc ctttagcgtc
     3901 cagccatcta gcaagcggtt cacctcctta ctgatgccgg gatagcccag ttcgtgccag
     3961 gatcgaagaa agccacggat ggttgctaga taccattctg ttgcaggcga cagcatcgac
     4021 cgataattaa tcaataccgt atcagtaatt tctctcgcgt cagtctcacg cagcatgtgc
     4081 tgaaaccgtt caagaatatt ttttgtatga gaagaagaac gattggatgc ataaaacgcc
     4141 agtgtacgca ggcagccatc tagcgtccca tctgacaagt aatggtgaat atcagcaaga
     4201 ttaatagtga tatttctatc caatgtccac gatgtgtcat acaacagaaa agagtagcca
     4261 ttggccctgg cacggattcg ttctggtact aacccaagta agaggttatt catggtcatc
     4321 cttgaagtcc tctggcaagc tagtgccgtt acctgcttgt aatgccaagg cggcctcaaa
     4381 acctttttgc cgaatgaaac gcttgttgta agtcgctgcg gttcccgagc catctctcca
     4441 gcccattaag tacgaacgaa gttgctcttg gcgttcctcg ctgacttgct cattcatggc
     4501 atccattctt tctgaaaact tgcgattcca agtgtggcgg agcgaatgtc ctgtcgctgc
     4561 atacaattgc ggtgatatag acctgactac ggaaaaaatt ttatgatatc cccctttgga
     4621 gataggattt cccagagttg gtccactctt gtaagttaca aacagatagt catttttctt
     4681 cgcatttctg actttccggc gatcttgaag gatataacta tgcagttctt gagctaagga
     4741 ttcagccaac ggtaagagcc gttctctagt tttagcatta ggctcattgg ttctagagtc
     4801 agcttgttca tctgctctcc taacgatacg gatcctgtta gcgctaaaat ctatgtcctg
     4861 tattcttata tttaataatt caccacctcg gatgccaaga taaaacagaa gtaagatcat
     4921 cagccgattg cggcgctgca catctattga gaaagggttt aactctgatc taggctggat
     4981 gacttcaaat agcgcgtcca attgaacatc actcaatgac ctgtcttgta agtcgtggtt
     5041 tctgcgcttt ttagatggtc ggcgcgtctt tatctgcgcc gccatagcat tgatcttttc
     5101 gacaacttcc agttctgtcg tctttaagat atgcatcgca taccagtgta aatagttggc
     5161 aaacgttgtt agccgggaat gctgtgtccc gttatcaacg atatcggtca tcgcatcggc
     5221 caaccaagag gtagaggaca cagttaatgg cccctttctt tgtttgcact gagcaaaatc
     5281 tcgcagatca tccagctcat gtggcttgaa gaagtctttg gtcaaaaacc gctgctcaag
     5341 gtcaatgtcg cgactctcta caaagcgtaa caacataacc aggttgttag ccgccgccaa
     5401 catcgttgaa aaagcatcct ctctattgcg gatttgagtt gttagaaaca gagtggggta
     5461 atacatggga agcccagaag cacgatccac taccatgcaa tagcgctcgc cactatccat
     5521 gacaaatgtt gtaactctgt agttcttttc catgcactca caccccataa acattacaca
     5581 attacaaggt acactttatg tgaaaaatca ccaatttata caagacactt tttccgacga
     5641 tagaaacaaa aaacccctga attaacaggg gcttgcgtat ggattcttta caaataactc
     5701 acaagttaac tctagaacgg aatgtcatca tcaaagtcca tcggcggctc gttagacggc
     5761 gctgccggag cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccaccc
     5821 tgcggctgct gtggctgacc ccaaccgccc tgcggctgac caccaccgat attgccacct
     5881 gccggagcgc caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc
     5941 acgacttctg tggtgtagcg atcctgaccg gattgatcgg tccatttacg ggtacgcagc
     6001 tgaccttcga tataaacctg agaaccttta cgcagatatt cgctggccac ttctgccagt
     6061 ttgccgaaca gcacaacgcg gtgccattca gtctgttctt tcatctcgcc ggtcgcttta
     6121 tcacgccagg attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg
     6181 tagcgtactt ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg
     6241 ctggccatgt tcgtgtctcc tgaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt
     6301 accattacca atagcgcttt tactatgttg tgacctcggt tccgggaaac aaacctggcc
     6361 agacattgtt acacaacact ccgggtaatg cattccaata ctgtatattc attcaggtca
     6421 atttgtgtca taattaaccg tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag
     6481 cgtttaatcc gggaaaggtg aatggataag atcgaagttc ggggcgcccg cacccataat
     6541 ctcaaaaaca tcaacctcgt tatcccccgc gacaagctca ttgtcgtgac cgggctttcg
     6601 ggttctggca aatcctcgct cgctttcgac accttatatg ccgaagggca gcgccgttac
     6661 gttgaatccc tttccgccta cgcgcggcag tttctgtcac tgatggaaaa gccggacgtc
     6721 gatcatattg aggggctttc tcctgccatc tcaattgagc agaaatcgac gtctcataac
     6781 ccgcgttcta cggtggggac aatcaccgaa atccacgact atttgcgttt gttgttcgcc
     6841 cgcgtcggcg aaccgcgctg tccggaccac gacgtcccgc tggcggcgca aaccgtcagc
     6901 cagatggtgg ataacgtgct gtcgcagccg gaaggcaagc gtctgatgct gctcgcgcca
     6961 atcattaaag agcgcaaagg cgaacacacc aaaacgctgg agaacctggc aagccagggt
     7021 tacatccgtg ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa
     7081 ctgcaaaaga aacataccat tgaagtggtg gttgatcgct tcaaggtgcg tgacgatctt
     7141 acccaacgtc ttgcggagtc gtttgaaacc gcgctggagc tttccggtgg tacagcggta
     7201 gtggcggata tggacgaccc gaaagcggaa gagctgctgt tctccgccaa cttcgcctgc
     7261 ccaatttgcg gctacagtat gcgtgaactg gagccgcgac tgttttcgtt taacaacccg
     7321 gcaggtgcct gcccgacctg tgacggcctt ggcgtacagc aatatttcga tcctgaccgc
     7381 gtgatccaaa accccgagct gtcactggct ggcggtgcga tccgtggctg ggatcgccgc
     7441 aacttctatt acttccagat gctgaaatcg ctggcagatc actataagtt cgacgtcgaa
     7501 gcgccgtggg gcagcctgag cgcgaacgtg cataaagtgg tgttgtacgg ttctggcaaa
     7561 gaaaacattg aattcaaata catgaacgat cgtggcgata cctccatccg tcgtcatccg
     7621 ttcgaaggcg tgctgcacaa tatggagcgc cgttataaag agacagaatc cagtgcggta
     7681 cgtgaagaat tagccaagtt tatcagcaat cgcccatgcg ccagctgcga agggacgcgt
     7741 ctgcgtcggg aagcgcgcca cgtttatgtc gagaatacgc cgctgcctgc tatctccgac
     7801 atgagcatcg gtcatgcgat ggaattcttc aacaatctca aactcgctgg tcaacgggcg
     7861 aagattgcgg aaaaaatcct taaagagatc ggcgatcgcc tgaaattcct cgttaacgtc
     7921 ggtctgaatt acctgacact ttcccgctcg gcagagacac tttccggcgg tgaagcccag
     7981 cgtattcgtc tggcgagcca gattggtgcg ggcctggttg gcgttatgta cgtgctggac
     8041 gagccgtcta tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac gcttatccat
     8101 ctgcgcgatc tcggtaatac cgtgattgtg gtggagcacg acgaagacgc gattcgcgcg
     8161 gctgaccatg tgatcgatat cggtcctggt gcgggtgttc acggcggtga agtggtcgcg
     8221 gaaggtccac tggaagcgat tatggcggtg ccggagtcgt taaccggaca gtacatgagc
     8281 ggtaaacgca agattgaagt gccgaagaaa cgcgttccgg caaatccgga aaaagtgctg
     8341 aagctgacag gcgcacgcgg caacaacctg aaggacgtga cgctcacgct gccagtcggt
     8401 ctgtttacct gcatcacagg ggtttcaggt tccggtaaat cgacgctgat taacgacaca
     8461 ctgttcccga ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat
     8521 cgcgatattc aggggctgga gcatttcgat aaagtgatcg atatcgacca aagcccaatt
     8581 ggtcgtactc cacgttctaa cccggcgacc tataccggcg tgtttacacc tgtgcgcgaa
     8641 ctgtttgcgg gcgtaccgga atcccgtgcg cgtggttata cgccaggacg tttcagcttt
     8701 aacgtccgtg gcgggcgctg cgaagcctgt cagggcgacg gtgtgatcaa agtggagatg
     8761 cacttcctgc cggatatcta cgtgccgtgc gatcagtgca aaggtaaacg ctataaccgt
     8821 gaaacgctgg aaattaagta caaaggcaaa accatccacg aagtgctgga tatgaccatc
     8881 gaagaggcgc gtgagttctt tgatgcggtg ccagctctgg cgcgtaagct gcaaacgttg
     8941 atggacgttg gtctgacgta cattcgcctg gggcagtccg caaccacact ttctggtggt
     9001 gaagcccagc gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg
     9061 tatattctcg acgagccgac caccggtctg cacttcgccg atattcagca actcctcgac
     9121 gtgctgcata aactgcgcga tcagggcaat accattgtgg tgattgagca caatctcgac
     9181 gtgatcaaaa ccgctgactg gattgtcgac ctgggaccag aaggcggcag tggcggcggc
     9241 gaaatcctcg tctccggtac gccagaaacc gtcgcggagt gcgaagcttc gcatacggca
     9301 cgcttcctca agccgatgct gtaatcgtta aggccgcttt ctgagcggcc ttttcctttc
     9361 agagttgtac cagcaattta cgcttttctt ccggtagtaa attcactgcc tgctgataag
     9421 acgcatccac cagataatag atttgcgaat ccggcagcga accgtcgaga tacacggtgc
     9481 tccagtgcgc tttattcaga tggcggctag gacgcacatc gctgtgctgc tgacgtagca
     9541 gctccgccag ctccgggctg gttttcagcg aaacagctgg gcgattttct acttctttca
     9601 ccatcgcaaa cagtacatct tccactttga tctgcgtcgc tttccagtcg ttatgcacgc
     9661 tctgttctgc gcctggtttt gccatgcaat attgtagcaa ctccgaaatg gtcatttttt
     9721 actccccttg tagtgtcgcg atgatgcgac gcgatccgcc gtggatgcga tgttccccca
     9781 gccagatgcc ttgccaggtg ccggtctgaa tacgcccttt atgtaccggc aatacaagcg
     9841 atgttcccag cattgaggat ttgatatgag aaggcatatc gtctgctccc tcatagtcat
     9901 gctcataatt tccgttgtcg ggaacggtgc ggaggaaaaa acgctccatg tcgtggcgta
     9961 cggtgggatc gcagttctca ttaagtgtca gagaggcgga ggtatgttgc agcaacagat
    10021 gcagtaagcc gatgttaacg cgcggcatat cagccagctg attcagaatt tcatccgtta
    10081 ccagatgaaa cccacgagat ttggcgctaa gcgtgagcgt cttttgatac cacatgtgct
    10141 gctccttgat aaaactctct taatcagttt gcagcaagac agcgaaagga taaaggtgtg
    10201 attaaaaaaa cagcattcgg gagagcgtca cgctctcccg ctctgtcagt attctgaatt
    10261 gacgatcacc tcttcaccaa acgcacccgc ttgtggcaag ggtttgtagg tagagttgga
    10321 ggcgcgcaga atgcggatac cacgagcgtc gacatcgcgt gcggcggtaa tatcgttatc
    10381 ggaatcgcca taaaaaattc ggatattttt atcctgcagc cattgcgatt ttgtattttg
    10441 ccctggttta tcgcccgcaa agatcaccgg attcatgttg gtgacaggaa tatgaaaatt
    10501 atccgccagc gtttttgaaa ccgtttctgt tttcgtcggg ctacgaccag tcacaaagaa
    10561 gatcgcgtca ccgcggcgta catgcatatc aatcagctgg cgagcgacct cttttggaat
    10621 gctgaattca tcccagccat tgttcatttt ttcccagaac acaggatttt tcagataatc
    10681 ttcgctttct ggtgagaagg tttttttgcc gcgccagaag cccgggctgg aaaaaagtac
    10741 cgtgtcatcg atatcaaacc ccaccgccat tggcggacgc cctgcgaggc tattttcaat
    10801 ttgtgcgacc gaaacccaat gaattggtgc ctgttcagca agcctggcaa cgttagtccc
    10861 agggttaagc ggtgaaggag atgaggccag ggcaacagcg gaactgttta gagcgaacaa
    10921 taagcaaacg gcactgattg cctgtgtgat cttgcgcata tttttcccta aatagtcagt
    10981 atgttgaaac tttttattgt gagatttgtt gcaaaaacca tctgaccata acgccagctg
    11041 ccggggaaag gaaggattat ctgcggtttt tgtgatgaaa agcaggatga ttatgtttaa
    11101 cccagtaaat acgttcctgt ctttttttgt ataaaaaatg tttattactc gatgtaagga
    11161 tacattaatt ttacgtttat tatgatgaat cctctgcaaa tgagcgaaat ttgatagaac
    11221 atttatctgc tgtacgattt aatgaactta atgatacagc attacaaaaa caatcgaagt
    11281 ttataaagat gattcctgat tgaccagaac cctatcctac aatggtgact aattttatta
    11341 ctccc
//
LOCUS       DAPHJE010000127        11272 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPHJE010000127 DAPHJE010000000
VERSION     DAPHJE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.128"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..677
                     /gene="gspC"
                     /locus_tag="RH222_003702"
     CDS             <1..677
                     /gene="gspC"
                     /locus_tag="RH222_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV3798655.1"
                     /translation="IINQPTNAFQSDFSLAALWRNENHAGVKDANPVAVNQETPKLSI
                     ALNGIVLTSNDETSFVLINEGSEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYE
                     KVTLHPGLPDIIKQPDSESQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQ
                     PGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVGELTGMNGL
                     RHERTQ"
     gene            661..2613
                     /gene="gspD"
                     /locus_tag="RH222_003703"
     CDS             661..2613
                     /gene="gspD"
                     /locus_tag="RH222_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV3798656.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYHSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2623..4104
                     /gene="gspE"
                     /locus_tag="RH222_003704"
     CDS             2623..4104
                     /gene="gspE"
                     /locus_tag="RH222_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV3798657.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4101..5297
                     /gene="gspF"
                     /locus_tag="RH222_003705"
     CDS             4101..5297
                     /gene="gspF"
                     /locus_tag="RH222_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV3798658.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFMHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5307..5744
                     /gene="gspG"
                     /locus_tag="RH222_003706"
     CDS             5307..5744
                     /gene="gspG"
                     /locus_tag="RH222_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV3798659.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     VVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5752..6261
                     /gene="gspH"
                     /locus_tag="RH222_003707"
     CDS             5752..6261
                     /gene="gspH"
                     /locus_tag="RH222_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV3798660.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVTNTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6258..6635
                     /gene="gspI"
                     /locus_tag="RH222_003708"
     CDS             6258..6635
                     /gene="gspI"
                     /locus_tag="RH222_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV3798661.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6628..7215
                     /gene="gspJ"
                     /locus_tag="RH222_003709"
     CDS             6628..7215
                     /gene="gspJ"
                     /locus_tag="RH222_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV3798662.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7208..8191
                     /gene="gspK"
                     /locus_tag="RH222_003710"
     CDS             7208..8191
                     /gene="gspK"
                     /locus_tag="RH222_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV3798663.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8206..9369
                     /gene="gspL"
                     /locus_tag="RH222_003711"
     CDS             8206..9369
                     /gene="gspL"
                     /locus_tag="RH222_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV3798664.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQKSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9366..9827
                     /gene="gspM"
                     /locus_tag="RH222_003712"
     CDS             9366..9827
                     /gene="gspM"
                     /locus_tag="RH222_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HDV3798665.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLGVIKPIDTYIAEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLAITLENGPDNTL
                     TIHPVTAPLENVSRWLTTAQVTYGIVIEDLQFTLAGNEEITLRHLSFREQQ"
     gene            9827..10504
                     /gene="gspO"
                     /locus_tag="RH222_003713"
     CDS             9827..10504
                     /gene="gspO"
                     /locus_tag="RH222_003713"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HDV3798666.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10533..11009)
                     /gene="bfr"
                     /locus_tag="RH222_003714"
     CDS             complement(10533..11009)
                     /gene="bfr"
                     /locus_tag="RH222_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV3798667.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11081..>11272)
                     /gene="bfd"
                     /locus_tag="RH222_003715"
     CDS             complement(11081..>11272)
                     /gene="bfd"
                     /locus_tag="RH222_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV3798668.1"
                     /translation="YVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRA
                     AREVMEDELMQLPEFKESA"
BASE COUNT         2936 a         2904 c         2712 g         2720 t
ORIGIN      
        1 agatcattaa tcaaccaaca aacgcatttc agtctgattt cagccttgcc gctttatggc
       61 gtaacgaaaa tcatgctggg gtaaaagatg caaatcccgt ggcagttaat caggaaacac
      121 ctaagctctc tatagcgctt aatggcattg tactcacatc gaatgatgag acttcttttg
      181 tcttaattaa tgaggggagc gaacaaaagc gatactccct caacgaagcc cttgagtcag
      241 cgccgggaac gtttattaga aaaataaata aaaccagcgt tgtgtttgaa actcacgggc
      301 attacgaaaa agtaactctc catccagggc tacctgacat catcaagcag cctgattcag
      361 aaagtcaaaa tgtactcgct gattatatta tcgccacgcc tatacgtgat ggagaacaga
      421 tatacgggct tcgattgaac cctcgtaagg gcctcaatgc atttaccacc agcttgctac
      481 aaccagggga tatcgccctc aggatcaata atctttcgct gacccaccct gatgaggttt
      541 cacaggcatt aagtttactg ctaactcaac aaagtgctca gtttacaatt cgtcgcaacg
      601 gcgtaccccg cttgataaat gtttccgtcg gggaacttac aggaatgaat ggactgcgtc
      661 atgaaaggac tcaataaaat cacctgctgc ttgctggcag cactactcat gccttgtgca
      721 ggacacgctg agaacgaaca atacggcgcg aacttcaata acgccgatat ccgccagttc
      781 gtggaaatag tgggtcagca tcttggcaaa acgatcctga tcgacccttc ggtacaggga
      841 accatttccg tacgcagtaa tgatacgttt agccaacagg agtactacca gttcttttta
      901 agtattcttg atctttacgg ttattccgtg atcacgctgg acaatggttt tctgaaagtg
      961 gttcgctcag ctaatgtaaa aacatcgcca gggatgattg ctgacagttc tcgtccaggc
     1021 gtaggtgatg agttggtcac ccgaattgta ccgcttgaga acgttcctgc tcgtgacctg
     1081 gcccccctgc tccgccagat gatggatgcg ggtagcgtcg gtaatgttgt gcattatgaa
     1141 ccctccaacg ttcttattct gaccggtcgt gcctccacca ttaataaact gattgaagtc
     1201 ataaagcgcg ttgatgtcat cggcacagag aagcagcaaa ttattcatct ggaatatgcg
     1261 tcagcggaag atctcgccga gattcttaat caattaatca gcgaaagcca cggtaaaagc
     1321 cagatgccag ccctcctctc cgcgaagatt gtggcggata agcgaaccaa ctctcttatc
     1381 atcagtggac cggaaaaagc acgccagcgc atcacttcat tactgaaaag ccttgatgtc
     1441 gaagagagcg aggaaggaaa tacccgggtt tattacctga aatatgctaa agccacgaat
     1501 ctggtggaag tgctaaccgg tgtttccgaa aagctgaaag atgaaaaagg gaatgcgcgt
     1561 aagccctcct cttctggcgc gatggataac gtcgccatta ccgccgatga acagactaac
     1621 tctctggtca ttaccgctga ccagtccgtc caggaaaaac tcgccacggt aattgcgcgt
     1681 ctggacattc gccgtgcaca ggtgctggtt gaggcaatca tcgttgaagt tcaggatgga
     1741 aatggactaa acctcggcgt gcaatgggcg aataaaaacg ttggcgcaca gcaatttacc
     1801 aataccggat taccgatttt taacgctgcg caaggtgtgg ctgattataa aaagaatggt
     1861 gggatcacca gcgcgaatcc tgcctgggat atgtttagcg cctacaatgg catggccgca
     1921 ggcttcttca atggcgactg gggagtactg cttaccgcgc tggccagtaa caataaaaat
     1981 gacatcctcg ccaccccaag catcgtaacg ctggataata aactcgcgtc cttcaacgtg
     2041 gggcaggatg tgccggtgct atccgggtca cagaccactt caggggataa cgtctttaat
     2101 accgtcgaac gcaaaacggt ggggacaaaa ctcaaagtta ctccgcaggt caatgaaggc
     2161 gacgcggtgt tgctcgaaat agagcaggaa gtctccagcg ttgactcttc ctctaactcg
     2221 acgctcggcc cgacgtttaa tacccgtact attcaaaacg ccgtgctggt caaaaccggt
     2281 gaaacggtgg tcctgggcgg attgctggat gatttttcta aagagcaagt gtcaaaggtt
     2341 cctctgcttg gcgatattcc tttagtgggg caactcttcc gctatacctc caccgagcgc
     2401 gctaaacgca acctgatggt atttatccgt ccgacgatta tccgtgacga tgatgtttat
     2461 cactcactgt caaaagagaa atacacccgt taccgtcagg agcaacaaca gcggatcgac
     2521 gggaaatcaa aagcgctggt tggctcggaa gatttgccgg tgctggatga aaacacgttc
     2581 aacagtcacg cccctgcgcc atcgtcacgg tgaggcattc atatgagaat tcactcaccg
     2641 taccccgcca gttgggcgct ggcacaacga attggttatc tctattcaga gggcgagatt
     2701 atttatctcg ccgatacgcc attcgagcgg ttactcgata ttcaacgtca ggttggccag
     2761 tgccagacca tgaccagctt gtcacaggct gattttgaag ctcggctgga agcggtattc
     2821 catcagaata ccggtgagtc gcaacagatt gcgcaggata tcgatcaatc cgtcgatctt
     2881 ctctcgcttt cggaagagat gcccgcaaat gaagatctcc tgaatgaaga ttcagcggca
     2941 ccggttatcc gcttgatcaa tgcgattttg agtgaggcca tcaaagaaac cgcctctgat
     3001 atccacattg aaacctatga aaaaacaatg tcgatccgtt ttcgcatcga cggcgttttg
     3061 cggacaattt tacagccaaa caaaaaactg gcggcactgc ttatctcccg aattaaggtc
     3121 atggctcgtc ttgatatcgc cgaaaaacgt attccacagg atggaagaat tagtttgcgt
     3181 atcgggcgac gtaacataga tgtccgcgta tccacactgc cgtccatcta tggtgaacgc
     3241 gccgtactcc gcctgctgga taaaaacagc ctccagcttt cattgaacaa cctggggatg
     3301 acggcagcgg ataagcagga tttagaaaat ctcattcagc ttccgcacgg tattatcctg
     3361 gtgacagggc cgacaggctc cggtaaaagc accacgctct acgccatcct ttcggcgctg
     3421 aatactcccg gccgcaatat tctgacggta gaagatcccg tggaatatga gctggaaggc
     3481 attgggcaaa cgcaggtgaa tacccgtgtg gatatgtctt tcgctcgcgg cctgcgcgcc
     3541 atacttcgcc aggacccgga tgtcgtcatg gtgggggaaa ttcgtgatac agaaaccgcg
     3601 cagattgcgg ttcaggcctc gctcaccggc catctggtac tctcaacact ccacactaac
     3661 agtgcatcag gcgcagtgac ccggctccgc gacatgggcg tcgaatcatt cctgctttcg
     3721 tcttccctgg cagggattat cgcgcaacgt ctggttcgtc gcctgtgtcc gcaatgccga
     3781 caattcacgc ccgtatcacc ccaacaagcg cagatgttta aatatcatca gctcgcggtg
     3841 acaacaattg gcactcccgt aggctgccct cattgccatc aatccggcta tcaggggcgc
     3901 atggcgatcc acgaaatgat ggtggtgacg ccggaattac gggccgctat tcatgaaaat
     3961 gtggatgaac aagcactgga gcgactagtc cggcaacaac acaatgcctt aatcaaaaat
     4021 ggcctgcaaa aagtgataag cggtgacacc tcctgggatg aggttatgcg cgtcgccagt
     4081 gccacgctgg agagcgaagc atgaattatc gctatcgcgc catgacccag gatggtcaaa
     4141 aattgcaagg gatcattgat gctaacgatg aacgtcaggc acgactgcgg ctgcgtgaag
     4201 aagggctttt cctgctggat attcgccccc aaaaaagttc gggagtaaaa acacgtcgcc
     4261 cgaggatcag ccatagtgaa ctgacgcttt tcacccggca gttggcaacc ttaagcgcag
     4321 cggcattacc cctggaagag agccttgccg taatcggtca acaaagcagt aataaacgac
     4381 tgggtgacgt gttaaatcag gtacgcagcg ccatccttga agggcatccc ctttccgatg
     4441 cattacagca ttttcccacg cttttcgatt cgctctatcg taccctggta aaagcgggcg
     4501 aaaagagcgg gctgctggcc ccggtgttgg aaaagctggc tgattacaat gaaaaccggc
     4561 agaaaatccg cagcaagctc attcagtcac tgatctaccc ctgtatgctc actacggtgg
     4621 cgattggggt cgtgattatt ctcctcactg ctgtcgtgcc caaaattacc gaacagttca
     4681 tgcatatgaa gcagcaactg ccgctgagta cacgcattct tttaggtctg agcgacacgt
     4741 tgcaacgtac cggcccgaca ttattagcga cagtgtttat tgtcgctgta ggtttctggc
     4801 tctggttaaa acgcggcaat aaccgccacc gttttcatgc catgttgctg cgcgttgcgc
     4861 tcatcggccc gctgatttgc gccattaaca gcgcacgcta tctccgcact ttaagtattt
     4921 tgcaatccag cggcgtccct ctgctggatg ggatgaattt gtccaccgaa agcctcaaca
     4981 accttgaaat tcgccagcgt ctggcaaatg cggcagagaa cgttcgccag ggtaacagca
     5041 ttcatctttc gctggaacaa accgcaattt tcccgccgat gatgctctac atggtggcct
     5101 ctggcgaaaa aagcgggcag ctcggcacat taatggtcag agccgcagat aaccaggaga
     5161 cactccaaca aaatcggatc gccttaacgc tctccatctt cgagccagca ctcattatta
     5221 cgatggcact gatcgtcctg tttattgtcg tgtcggtact ccaacctctt cttcaactta
     5281 actcaatgat taattaagga aagcatatgc gcgcaacgga taagcaacgc ggttttacat
     5341 tactggaaat tatggtggtg atcgtcatta tcggcgtact tgccagcctg gtggttccta
     5401 acctaatggg caataaagaa aaggcggata agcaaaaagt cgtcagcgat attgtggcgc
     5461 tggaaaacgc ccttgatatg tacaaactcg acaatcatca ctatccaacc acaaatcagg
     5521 ggcttgaatc cttagtagaa gcgccgacac tgccaccgct ggccgcaaac tataacaagg
     5581 aaggttatat caagcgtctg cctgccgatc cctggggcaa tgattatgtc ctcgttaatc
     5641 ctggtgaaca tggtgcatac gatctgcttt cagcagggcc cgatggtgaa atgggaaccg
     5701 aggacgacat caccaactgg ggtttgagca agaagaaaaa gtaagtcagc gatgaatcag
     5761 caacgcgggt ttaccctgct ggagatgatg ctggtgctgg cgctggtcgc tatcacggca
     5821 agcgtggtgc tcttcacata tggccgggag gatgtcacca acactcgtgc gcgcgaaaca
     5881 gcggcgcggt ttaccgctgc acttgaatta gccatcgacc gcgccacgct cagtggtcag
     5941 cctgtcggta tccatttctc tgattcagca tggcgcatta tggtaccggg caaaacacca
     6001 tcagcctggc gctgggttcc attacaagaa gatgccgcag acgagagcca aaatgactgg
     6061 gatgaagaac tctcaattca tcttcagcca tttaaacctg atgacagcaa ccagccacag
     6121 gttgtgattc tggccgacgg acaaatcacg cccttctctt tgttgatggc taacgccggg
     6181 accggtgagc cgctcctgac gttagtctgt tcaggctcat ggcccctgga tcaaacgctc
     6241 gccagagata cccgcccatg aacaaacaat cagggatgac actgcttgaa gtcttactgg
     6301 cgatgagtat tttcaccgct gttgctctga ctttaatgag cagtatgcaa gggcaacgaa
     6361 atgccatcga gcgaatgcgt aacgagacgc tggcattgtg gatagcggat aaccagcttc
     6421 agtcacaaga ttcattcggc gaggaaaaca cgtccagttc tggcaaagag ttaataaatg
     6481 gcgaagagtg gaactggcgc agcgatatcc actcaagcaa agacggcacg cttctggaac
     6541 gcaccataac ggtgacatta cccagcggtc agacaacctc gcttacgcgt tatcaaagca
     6601 tcgataataa atccgggcag gcacaagatg attaatcgcc agcagggttt cacattgctg
     6661 gaggtcatgg cggcgctggc gattttctca atgcttagcg tgctggcgtt tatgattttt
     6721 tcgcaagctt ccgagttgca tcaacgctca cagaaagaaa tccagcagtt caatcagcta
     6781 cagcgcacca tcaccattct tgataacgat ctcctgcaac tggtcgcccg gaggaatcgg
     6841 agtacggaca aaatcatggt gttgggtgaa gaagctattt ttaccacgca aagtcgcgat
     6901 ccgctggctc ctctcagtga agcacaaacc ttactcactg ttcactggta tctgcgaaat
     6961 catacgctct accgtgctgt tcgtacttct gtggatggca ggaaggatca acccgcccag
     7021 gcgatgctgg aacatgtcga gagctttctt ctggaaagta acagcgggga aagccaggaa
     7081 cttcctctct cagtcacgtt acatctgcag actcagcaat atggggggtt acagcgccgt
     7141 tttgcattac ctgagcagtt agcgagagaa gagtcgccag ctcagacgca ggccgggaat
     7201 aataaccatg aataatgaac aacgcggcgt cgcgctgctc attgtactca tgttactggc
     7261 gctcatggct gctctggcag cagacatgac gctcagtttt cacagccagc ttcagcgaac
     7321 ccgccaggta aaccatcatc tgcaacggca atatgatatt gaactggcgg agaaacttgc
     7381 gcttgccagc ctcacccagg acgttaaaga taacgacaga cagaccacgt tgcagcagta
     7441 ctgggcacag ccacaacagt tgcagctgga agatggcaat acagtgaagt ggcagttacg
     7501 cgatgcccag cactgtttta atctcaatgc gttagcaaaa atctctgacg acccgctggc
     7561 ttcccctgat ttccccgccc aggtattcag tgcgctgctg attaacgccg gtatcgatcg
     7621 cggaaacacc gatgagatcg tgcaatcaat cgctgactat attgatgtcg atgactcacc
     7681 acgctttcac ggcgcagaag atagctttta ccaaagccag acgccgcccc ggcatagtgc
     7741 caatcagatg ctttttctga ctggcgaatt acgtcagata aaaggcataa ccgaaaacat
     7801 ctatcagcgg cttatcccgt atgtctgcgt tctcccgacc acggaactta gcatcaatct
     7861 taatatgcta acggagaacg atattccgct cttcagagcg ttattcctca ataacatcac
     7921 ggatgctgac gctcgggtct tattgcaaaa aaggccgagg gaaggctggc taaccacgga
     7981 tgcatttctt tactgggcgc agcaggattt ctccggtgtg aagccactgg ttgctcaggt
     8041 gaaaaggcat ctctttccct atagccgcta ttttacgctg agtacggaaa gtatcagcga
     8101 cgaacaatcc cagggatggc aaagccatat ttttttcaac cgtaaacagc agagcgccca
     8161 aatctatcgg cgtacgctgc aactctacta atcataagga tcgtgatgcc tgaatcgttg
     8221 atggtcatac gttcttcctc gacgctgcgt aagcattggg aatggatgac gttctccgca
     8281 gatagcgtat cttcggtaca tacgctcacg gacgatcttc cgctggagtc gctggccgat
     8341 cagcccggtg ccggaaatgt tcatcttttg atcccaccag agggcctttt atatcgttcc
     8401 ctcacgctgc ccaacgccaa atacaaactc accgcacaaa cattacaatg gctggcagag
     8461 gagacgttac ccgacaacac ccaggactgg cactggacgg ttgtcgataa acaaaacgaa
     8521 agtgtcgagg tgataggcat tcagtcggag aaactcagtc gctatcttga gcgtttacat
     8581 actgccggac tcaacgtgac gcgggttttg ccagatggct gctatcttcc ctgggaagta
     8641 gatagctgga ctctggttaa ccagcaaaaa agctggctca ttcgcagtgc cgcacatgcc
     8701 ttcaacgaac tggatgaaca ctggttacag catcttgctg cccagtttcc gcctgagaat
     8761 atgctttgtt atggtgtagt acctcacggc gttgcagcgg ccaatccact tatccagcat
     8821 cctgaaatac catcactgag tttgtattct gccgatattg cattccagcg ctatgacatg
     8881 ctgcatggta tttttcgtaa acagaaaact gtaagcaaat cgggaaaatg gctcgcccgc
     8941 ctggcagtaa gctgtctcgt tctcgccatt ctttcttttg ttggcagccg gagcatcgcg
     9001 ctctggcata cgcttaagat tgaagatcaa cttcagcaac aacagcaaga aacctggcaa
     9061 cgttatttcc cgcagatcaa acgcacccac aactttcatt tttactttaa gcagcaactc
     9121 gctcagcaat atcctgaagc tgtaccgttg ctctatcatt tacaaacact tctgctggaa
     9181 caccctgaac tgcagttaat ggaggcgaac tatagccaga agcaaaagtc gctgacctta
     9241 aaaatgagtg ctaagagtga agccaatatc gatcgtttct gtgagttaac acagtcctgg
     9301 cttccgatgg aaaaaacaga aaaagatccg gtcagtggtg tatggacagt aaggaactca
     9361 gggaaatgat taaatcatgg tgggcagaaa aatccacatc ggaaaaacag atcgttgcgg
     9421 cactggcggt tctctcgctc ggcgtttttt gttggcttgg ggtgataaaa ccgatagata
     9481 cctacattgc agagcatcag tcccacgcgc aaaaaattaa gaaggacatc aaatggatgc
     9541 aggatcaagc gagcacccat ggtttactcg gtcatcctgc gcttacgcag cccatcaaaa
     9601 atatccttct cgaggaagca aaacgtgaga atttggctat cacgctggaa aacggcccgg
     9661 acaatactct gacaattcat cctgtcacag ctcccctcga aaacgtctcc cgctggctca
     9721 ctacggcaca ggtaacatac ggtattgtta ttgaagacct tcagtttact cttgccggaa
     9781 acgaagagat caccctcagg catttgtcct tcagggagca acaataatga caatgctact
     9841 gccgctcttc attctggttg gttttattgc aggttatttt gttaatgcca tcgcctatca
     9901 cctctcgccg ttggaggata aaacggcgtt aacgtttcgc caggtattgg ttcatttcag
     9961 gcaaaaaaaa tatgcctggc atgatacagt gcccctgata ctttgtgttg ctgctgctat
    10021 cgcctgcgcc ctggcacctt tcacgcccat cgtgactggc gcactctttc tctatttctg
    10081 tttcgtactc acgctcagtg ttattgattt tcgcactcag ctcctgcccg acaaactcac
    10141 cttaccgctg ctctggcttg gcttggtatt taatgcgcag tatggattaa ttgatttaca
    10201 tgatgcggtt tacggcgcgg tagcgggcta tggggtgctg tggtgtgttt actggggcgt
    10261 ctggttagtt tgtcacaaag agggattggg ctacggtgat ttcaagctac tggccgctgc
    10321 aggcgcatgg tgtggctggc aaacgttgcc aatgatactg ctgattgcct cgctgggtgg
    10381 cattggttac gccatcgttt cacaacttct gcaacgccgg actataacca ctatcgcatt
    10441 cggcccgtgg cttgcgctcg gcagcatgat aaacctgggg tatctggcct ggatctctta
    10501 ttaaccggga gggttctccc tcccgacacg gctcaacctt cttcgcggat ctgtgcttgc
    10561 agataatttt gcaggcccat cttctgaatc agatcaagtt ccgtttccag ccagtcgata
    10621 tggccttctt catcacgcaa aatttctatc atcatatcgc ggctgacgta atcatgaacg
    10681 ctatcggcat aaccaattgc ctcacgcaaa ttcttcgcgc catccagctc aagtgccaga
    10741 tcagaacgca gcatttcctc aacatcttca ccaatgttca gtttgcccag gtcctgtaag
    10801 tttggaagac cttccagaaa aagaatgcgc tcaatataac gatcggcgtg tttcatctca
    10861 tcaatggatt catgatactc cacatcattg agacgtttga gaccccagtt tttaaacatt
    10921 cgggcatgga gaaagtactg attgattgcg acaagctcat ttcccaacag tttgttgaga
    10981 taatttataa ctttagtatc acctttcatt ttttgactcc ttcgcttcca ctctttgaag
    11041 agtagaacgg cttacgagta agtcaaaaaa cacactccgt ttatgcggac tccttaaact
    11101 ccggcagctg cattaattca tcctccatca cttcacgcgc ggcccggaca catttaccgc
    11161 actgattgcc gaccggaata aattttttta attgctggaa cgagtgaggg gaaaactggc
    11221 gcacagcctg acgaattttt ttatcgctga taccattaca aagacaaacg ta
//
LOCUS       DAPHJE010000128        11261 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPHJE010000128 DAPHJE010000000
VERSION     DAPHJE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.129"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..605
                     /locus_tag="RH222_003716"
     CDS             3..605
                     /locus_tag="RH222_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV3798669.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(607..1128)
                     /gene="ruvC"
                     /locus_tag="RH222_003717"
     CDS             complement(607..1128)
                     /gene="ruvC"
                     /locus_tag="RH222_003717"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV3798670.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1163..1903)
                     /locus_tag="RH222_003718"
     CDS             complement(1163..1903)
                     /locus_tag="RH222_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3798671.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1932..2384)
                     /gene="nudB"
                     /locus_tag="RH222_003719"
     CDS             complement(1932..2384)
                     /gene="nudB"
                     /locus_tag="RH222_003719"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV3798672.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTCNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2502..4274)
                     /gene="aspS"
                     /locus_tag="RH222_003720"
     CDS             complement(2502..4274)
                     /gene="aspS"
                     /locus_tag="RH222_003720"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV3798673.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4584..5150
                     /locus_tag="RH222_003721"
     CDS             4584..5150
                     /locus_tag="RH222_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3798674.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            5147..5965
                     /locus_tag="RH222_003722"
     CDS             5147..5965
                     /locus_tag="RH222_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV3798675.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6018..6413
                     /locus_tag="RH222_003723"
     CDS             6018..6413
                     /locus_tag="RH222_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV3798676.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6454..7197
                     /gene="cmoA"
                     /locus_tag="RH222_003724"
     CDS             6454..7197
                     /gene="cmoA"
                     /locus_tag="RH222_003724"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV3798677.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7194..8165
                     /gene="cmoB"
                     /locus_tag="RH222_003725"
     CDS             7194..8165
                     /gene="cmoB"
                     /locus_tag="RH222_003725"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV3798678.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8330..10759)
                     /gene="torZ"
                     /locus_tag="RH222_003726"
     CDS             complement(8330..10759)
                     /gene="torZ"
                     /locus_tag="RH222_003726"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV3798679.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFDDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGHASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAIADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10784..>11261)
                     /locus_tag="RH222_003727"
     CDS             complement(10784..>11261)
                     /locus_tag="RH222_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HDV3798680.1"
                     /translation="ELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQR
                     LALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDD
                     TYCAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2540 a         3022 c         2785 g         2914 t
ORIGIN      
        1 acgtgaatat taattaccct gctgaatatg aaattggaga tatcgccttt acatgtatag
       61 gtgctgcctt atttggtcaa atatcagctg catcaaattg ttggagtaat cacgtcggga
      121 tcattatcgg tcataacggt gaagacttcc tggttgcaga aagccgggtt cccctctcaa
      181 ccatcactac gctatcccgt tttattaaac gctctgctaa tcaacgctat gctataaagc
      241 gattagacgc tgggctgaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc
      301 ggctacgcaa actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa
      361 aatttgtctt tgatatttat aaagaggcgc tatgtattcc ggtgggtgaa atagagacgt
      421 ttggagaatt gttaaatagc aatccgaatg caaaactcac tttctggaaa ttctggttcc
      481 tgggttctat tccgtgggag cgtaaaaccg ttacgccagc cagtttgtgg catcatccgg
      541 gtttggtgtt gattcatgcg gagggagtgg aaacgcctca gccggaactg accgaggcgg
      601 tataacttaa cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcatcgc
      661 attctgacta acgtggcagt gggtgatggc aatcgccaac gcatcagcgg catccgcctg
      721 tggattagcg ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttttcggc
      781 gctaccaata cccaccaccg tttgcttcac ctgacgtgca gcatattcaa ataccggcaa
      841 ctcctgattc accgccgcca caatcgccac gccgcgcgcc tggcccagtt tcagggctga
      901 gtcagcgttc tttgccataa agacttgttc aatggcgaaa taatcaggct ggaactgggt
      961 gatgatttcc gtcacgcccg catagatgag tttcagacga gacggtaaat catccacttt
     1021 ggtgcgtatg catccgctac ccaggtagga cagttgccta cctacctggc ggatgacgcc
     1081 gtagccggtc acgcgcgaac ccggatcaat gccgagaata atagccatca cgcgtctccg
     1141 ttttgctgtt tagcaggcct catcagagag tcgctgcgac ctcatcagag atttcaccgt
     1201 tatggtaaac ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt
     1261 tcggtgcggt ttctgcatcc atatcagctt tggtagacgg gatcatggaa acttccgcgc
     1321 tgtctgcttt cagacctgcc gcttccagag cgtcgcgcac tttacccatt tcttcccatg
     1381 cagtgtagac atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca cctgcttcca
     1441 gtgctgcttc catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc
     1501 tgaacagata agctacggaa ccatcagtac cgaggttacc gccacatttg ctgaatgcat
     1561 gacgcacttc agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg
     1621 tgccgccagg accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc
     1681 ccacaccacg tgcaattgcg cggttcagtg tgtcacgggt catgttgtta gacagtgctt
     1741 tatccaccgc cgcacgcaga cgcgggttag cgtccggatc gccaccgccc agcttagccg
     1801 cggttaccag ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag
     1861 ctttacgatg tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata
     1921 gccctgcctg ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag
     1981 gacttagtga gcgccgccgc agcaggcgca tcaagccact tgtaagccag atgttcagtg
     2041 aaaacgatct gccgctcgtg cggaagcgca agacagaacc atgattccgt attacacgtc
     2101 acgcccggcg catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga
     2161 cagtcaatta aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg
     2221 cgcatggcag cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag
     2281 aaatcgggat cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcgtagatg
     2341 accactaaga tcgaaacggg acgcttatac actttatcct tcacgctgcc tcttcgttga
     2401 ctgccttcgc tcatcccatt cacatagtta tctatgctca tgggagttca ctcagttgcc
     2461 gcctcgatgc aacgcgaatg atttcgtgta tttgagtcat atcagttatt ctcagccttc
     2521 ttcacaacct gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca
     2581 gtcatcagac acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg
     2641 gtgccggtca gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga
     2701 gtaccgtatt tcagagcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg
     2761 atacccagaa taccaaacac cgtctgctgc atatcaccat tatggatacg tactgaacca
     2821 ccgcccactt cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttccggt
     2881 gcagctttca gttctgcagc cgtcatatct ttcggtgagg tgaacggatg gtgcattgcc
     2941 gtcaggccgc cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc
     3001 catttgcttt cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcacccatc
     3061 gcgtcggcaa caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg
     3121 gcagtacgat ccaggatgtc ttcgatgatt tctgcattaa ggaacttcgc taccgggctg
     3181 ttgatacctt ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg
     3241 ccgtagattt taacgaagtt accgtattcg tcgatctgct tacgggtcag cgatgcgccg
     3301 cccggaacgc gcagagccgc tacgcgacct ttcggatcgt tcgccggacc tgcaaataca
     3361 gcaaactcaa cagatttcag cagatcagca acgtcagtca gttccatcgg gttacgcaga
     3421 tccggtttat cagaaccata acggcgttct gcttccgcaa aggtcattac cgggaaatcg
     3481 cccagatcca cacccttcac ttccagccac agatgacgca ccagcgcttc catcacttca
     3541 cgcacttgcg gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga
     3601 cggtcagcac gcaggtcttc gtcacggaag catttaacga tctgatagta acggtcaaaa
     3661 ccggacatca tcagcagctg tttgaacaac tgcggggatt gcggcagtgc gtagaattta
     3721 cctttgtgca cacgagaagg caccaggtag tcacgcgcgc cttccggcgt ggctttggtc
     3781 agcatcggag tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg
     3841 gtgattttag cgcgggtctt caggcgctga gccatttccg gacgacgcag gtcgaggtag
     3901 cggtatttca gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct
     3961 gcgcggttga tgatagtcag cgaggacgcc agcacttcga tttcgccggt cgccatatcg
     4021 cggttaatat ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat gcagaactca
     4081 ttacgcagtt cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc
     4141 acgatacctt cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg
     4201 ttgacccaac cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa
     4261 tattctgtac gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca
     4321 ggtgaccaag tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat
     4381 actggaaatt ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt
     4441 tatatcgggc ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca
     4501 taaaaattaa caaaatttgt cgttccgcca tcggctaatc gcattaaggt gtgaggcacg
     4561 attttgtttt ttcaggagtc attatgcttg aacttaatgc taaaaccact gcgctggtgg
     4621 tgatcgattt acaagaaggc atcttacctt ttgccggtgg tccacatact gccgatgagg
     4681 tagttaatcg cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc
     4741 tggtgcgcgt tggctggtct gccgattacg ctgaagcatt aaaacagccg gtcgatgcgc
     4801 cctcaccggc taaagtgttg cccgaaaatt ggtggcaaca tcctgctgca ttaggtgcaa
     4861 ccgacagcga tatcgaaatc atcaaacgtc aatggggtgc gttttacggt acggatctgg
     4921 agttgcaatt acgccgccga ggtatcgata caatagtgtt atgtgggatc tcgaccaata
     4981 tcggtgttga atccaccgcc cgcaatgcct gggaacttgg ctttaatctg gtgattgccg
     5041 aagacgcctg tagtgccgcc agcgccgagc agcacaataa cagtatcaac catatctacc
     5101 cgcgcatcgc ccgtgtgcgt agcgtggaag agatcctcaa cgcgttatga tttacatcgg
     5161 tctaccgcaa tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc ttgaagagta
     5221 tgcccgccac tttaactgcg tggagggcaa caccacgctt tacgccctgc cgaaacccga
     5281 ggttgtcctg cgctggcgtg agcagaccac agatgacttc cgcttctgtt ttaagtttcc
     5341 ggcgaccatt tcgcatcagg cagcattacg gcattgcgat gatttagtga ctgaattttt
     5401 gacccgcatg tcaccgttgg ctccgcgcat tgggcaatac tggctgcaac tgcctgccac
     5461 attcggccca cgggagctgc ctgcgctttg gcattttctc gattctcttc ctggcgaatt
     5521 taattatggc gtggaagtcc gccatccaca gtttttcgcc aaaggagaag aggaacaaac
     5581 gcttaatcgc ggtttacatc agcgcggcgt taatcgggtg attttagaca gccgcccggt
     5641 tcatgcagca cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt
     5701 tccggtacat gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga
     5761 tatgacgcaa aaccgtgaat tatttcaggt ctggttacaa aaattagcgc agtggcatca
     5821 gaccactacg ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt
     5881 acataccctg tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat
     5941 tccacagcaa tcttctcttt tctgaatttg ccacctatca tagacaagtg caatcggcca
     6001 ttttaaaggg agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa
     6061 tgaagttctc ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg
     6121 gtggttttag cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc
     6181 ccatcgcgat tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc
     6241 atatttgcgg catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc
     6301 gtctgtttcg ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg
     6361 tgctggcgaa tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa
     6421 tacgccactt tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct
     6481 gcccctatcg ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg
     6541 gatatgatcc agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta
     6601 gccgagcgct tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg
     6661 gcgacgctct cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac
     6721 aactccccgg cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg
     6781 ccagtagacg tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg
     6841 gtgctgaatt ttaccctgca attcctggaa ccttccgagc gccaggcgtt actggataaa
     6901 atttatcaag ggctgaaccc cggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa
     6961 gatgccaaag ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt
     7021 tacagcgaac tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat
     7081 tccgtggaaa cccataaagc acgcctgcat aacgccggtt ttgagcatag tgagctgtgg
     7141 ttccagtgct ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg
     7201 actttggtaa cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc
     7261 tgcccgcgca gattgctaac tggcagcgcg agcagcagca cgggttgttt aagcagtggt
     7321 caaatgcggt ggagtttcta cctgaaatta aaccgtatcg tcttgattta ttgcatagcg
     7381 ttacggcaga aagcgaagag ccactgagcg cagggcaaat taaacgcatt gaaacgctga
     7441 tgcgcaacct gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata
     7501 ccgaatggcg ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg
     7561 gacgcaccat tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg
     7621 caggcgcgca cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag
     7681 cggtgcgtaa actactgggt aatgatcagc gcgcgcattt gttaccgtta ggtattgaac
     7741 aacttccggc actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc
     7801 gttcaccgct ggagcatcta tggcagttga aagatcaact ggtcaatgaa ggtgaactgg
     7861 tgctggaaac gctggttatt gatggtgatg agaacacggt gctggtacca ggcgatcgtt
     7921 acgcgcaaat gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga
     7981 agaagtgtgg ttttgtagat atccgcattg cggatgtgag cgttaccacc acagaagagc
     8041 agcgacgcac cgaatggatg gttaccgaat cgctggccga tttcctcgac ccacatgatc
     8101 cgggtaaaac ggtggaaggt tatccggcac ctaaacgcgc ggtgctgatt gcgcgcaagc
     8161 cgtaaaggtc tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc
     8221 ttgctaattc aatatattgc agggactatg taggcctgat aagcatagcg catcaggcag
     8281 ctttacgttt gcataacctc agcgcccgtt tccgggcgct attcacgtct tacgcctgaa
     8341 cagctggctg atcaaacgcc gttaacttcg gtgcattgcc cgtatatttt tcgatataca
     8401 ccaacgctga gttaccggca caggcatttg ccagctgcga gctggggata tccgccgtta
     8461 acacgttcgc actgccgttt ttacacaagc cattttccag atctggccat gcaccttcat
     8521 gcacgcatac cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac
     8581 cacgtttgtt ccagacacgc accagatcgc cattcgcaat accaaagcgt gcagcatctt
     8641 cggtgtgaat agtgattggt tcacgatcgg cgatcgcata ttttttacgc agttccgcat
     8701 agttaagctg actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgt aactgcttct
     8761 cgtcggcggt acctttccat tcatcaggag ccagccaggt tgggtgtgcc gggcaatcct
     8821 tatagccaaa tttttccaac gtttttgaat agatttcaat tttgccgctt ggcgtaccca
     8881 gcgcattttt caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct
     8941 cgctgcggcg catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag
     9001 tgacgcgttg cgcacgggca cctttctgag cggcatcata gaaaaatttc agccacgcca
     9061 tttcatcttt accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat
     9121 cggcaaacac gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg
     9181 gcacaatatg ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg
     9241 tggtgatcgg taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa
     9301 cgacgatcat ctccggtttc tgccatgctt taatcagacg attagtatcc tggtgatggg
     9361 taaagttacc gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac
     9421 cgttatgctg atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa
     9481 tcgccgtcat tccgccatca tcagcggcct cgctggcatg tccggcaatc gccgcagaca
     9541 tttccggcag cacgccgcca acacgcgtcg ggttgccacc gttggagtaa tgataagaga
     9601 aaccaaaacc gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca
     9661 tccagtgttt ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg
     9721 tgcgattcgc cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa
     9781 tttccgccgc ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt
     9841 caaactgcgg atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg
     9901 tcatcagggt atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga
     9961 tccaggtggc attatcgtca aagaattcga tggtttcgga gcggatagga tcaatggcaa
    10021 tcactggttt gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac
    10081 tgctccaggc aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc
    10141 tgttttccag aatcagcggc caggaggtct gctgttcata cacctctaca gaaccgacca
    10201 catgcggcat gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac
    10261 cgcccgccag gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag
    10321 agcgccagcc gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgat
    10381 catgttgctc atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt
    10441 cgccacgacc tttcgccggt tgcagtggat tatcgagata gctttttctc accatcggat
    10501 gctgaatacg cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gaattcggta
    10561 tggttttcgc cagcgcgcct gttgaagaaa caatcttgcc gtccttcact tctacgttca
    10621 tcgctcccca acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga
    10681 gcggtgctgc cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt
    10741 cacgtcttgt taatgtcata acttcctccc tgatcaacga ggatcactgt ttctcggtaa
    10801 tatctttggc gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt tcgctcatgc
    10861 tggttcgtgc ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg
    10921 cggcaatagg ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc
    10981 atagcggttt acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac
    11041 gccattgatt gccgtattcg tcagcctgcc attccccgtt tacagtgagc gccttgatac
    11101 cttcttcact taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt
    11161 cactgccctg catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa
    11221 cgacggtgag atcggttcca ggattcacgg tagccagctc g
//
LOCUS       DAPHJE010000129        11136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPHJE010000129 DAPHJE010000000
VERSION     DAPHJE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.130"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..279
                     /locus_tag="RH222_003728"
     CDS             <1..279
                     /locus_tag="RH222_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV3798681.1"
                     /translation="ALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITA
                     RADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(399..578)
                     /locus_tag="RH222_003729"
     CDS             complement(399..578)
                     /locus_tag="RH222_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV3798682.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            652..2013
                     /gene="pabB"
                     /locus_tag="RH222_003730"
     CDS             652..2013
                     /gene="pabB"
                     /locus_tag="RH222_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV3798683.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLNSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2017..2595
                     /locus_tag="RH222_003731"
     CDS             2017..2595
                     /locus_tag="RH222_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV3798684.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2779..4143
                     /gene="sdaA"
                     /locus_tag="RH222_003732"
     CDS             2779..4143
                     /gene="sdaA"
                     /locus_tag="RH222_003732"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV3798685.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4274..5872
                     /locus_tag="RH222_003733"
     CDS             4274..5872
                     /locus_tag="RH222_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3798686.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5876..7438)
                     /gene="yoaE"
                     /locus_tag="RH222_003734"
     CDS             complement(5876..7438)
                     /gene="yoaE"
                     /locus_tag="RH222_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV3798687.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHS
                     LFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRR
                     ARGSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHA
                     LQQALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVR
                     IVKEQPAHDEDE"
     gene            7901..8872
                     /gene="manX"
                     /locus_tag="RH222_003735"
     CDS             7901..8872
                     /gene="manX"
                     /locus_tag="RH222_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV3798688.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8935..9735
                     /gene="manY"
                     /locus_tag="RH222_003736"
     CDS             8935..9735
                     /gene="manY"
                     /locus_tag="RH222_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV3798689.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9748..10599
                     /gene="manZ"
                     /locus_tag="RH222_003737"
     CDS             9748..10599
                     /gene="manZ"
                     /locus_tag="RH222_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV3798690.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10654..11112
                     /locus_tag="RH222_003738"
     CDS             10654..11112
                     /locus_tag="RH222_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV3798691.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2712 a         2821 c         2828 g         2775 t
ORIGIN      
        1 gccctggatt tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa
       61 agcacggcga agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct
      121 gatattacgg cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt
      181 ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc tgaatacttt
      241 tcaacatata tgcaggaaga actaaaagca ctggtctgag ttaaatttat atcagcataa
      301 atgggtcagg acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaatgat
      361 catcggcttc tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa
      421 cgcgccacga ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt
      481 gcctggccgc tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc
      541 tgctgttcat gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt
      601 ggcgctaatt atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg
      661 ttatctcccg ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc
      721 cgcttaagcc acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat
      781 agccgctttg atattgtggt cgccgatccg atttgcactt taaccacttt cggtaaagaa
      841 accgttgtta gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc
      901 cagcaggtgc tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag
      961 ggcggcgcac tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa
     1021 attgcggaac aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc
     1081 attgtcgacc accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt
     1141 cgggcctggc tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac
     1201 tggcaatcca atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat
     1261 ctgaacagcg gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct
     1321 ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct
     1381 tttttacgtc ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt
     1441 gataatagtg aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct
     1501 caggaagata gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat
     1561 ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta
     1621 aaagtaccag agctgttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact
     1681 ataacggcgc gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct
     1741 ggtggctcaa taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa
     1801 ccgcagcgac gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg
     1861 gataccagca ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg
     1921 ggcggtggaa ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa
     1981 gttaataaga tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt
     2041 gattattttt tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat
     2101 catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg
     2161 actcagcgtt caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca
     2221 gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc
     2281 gctataccgc cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact
     2341 ggctatcagg taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt
     2401 gaagatgaag tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt
     2461 tatcaccctt tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac
     2521 gaacagtatt ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt
     2581 ggtgtgaaac cctgactata cttatcttta catctacaaa acactacttg agacaatcat
     2641 cgcaatatta gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag
     2701 tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac
     2761 cttgtcagga gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc
     2821 tcatcttccc ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc
     2881 gaaaaaggct tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg
     2941 ctgacgggta aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa
     3001 cctgccaccg tggatatcga cagtattccc ggttttattc gcgacgtaga agagcgcgaa
     3061 cgtctgctgc tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgtgt
     3121 tttcataacg gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc
     3181 gatgaagtcg tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa
     3241 gaacactttg gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc
     3301 accgaactgc tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag
     3361 aacgaactgg cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa
     3421 accatgcagg catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg
     3481 cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgctgg tttccagcga taaactgtct
     3541 aacgatccga tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa
     3601 aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg
     3661 gcagtgctgg cttactatga ccactttatt gaatcggtca gcccggacat ctatacccgt
     3721 tactttatgg cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt
     3781 gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt
     3841 gcagaactgc tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa
     3901 cacaaccttg gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag
     3961 cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc
     4021 agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac
     4081 atgaacgcca aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac
     4141 taatacttct tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct
     4201 gctgtaatat tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta
     4261 cagggtggcc cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat
     4321 gattgtttgt acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat
     4381 ttcacagcgt aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga
     4441 attagataaa gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg
     4501 tctgccctgc tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt
     4561 gcgatccatt ggcctggtgc aagacggcac actttattgc tccagcattt ttggttatcg
     4621 caatgtgccc gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct
     4681 gacgatcgac cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc
     4741 gggcagtagc aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct
     4801 ccttgagcca caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt
     4861 gctctatggt aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca
     4921 ggtttcttcg caacgctttc cttttaccat taacgttaat ggtccggggg ctacggcgct
     4981 ggcatggcac tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg
     5041 ctacatcgcc tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa tcaatctggg
     5101 cctggcgcaa catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca
     5161 atgtattggt gtagagattt tgctgcgctg gaacaatccg cgtcagggct ggatttcacc
     5221 ggatgtgttt attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt
     5281 gatggcagaa accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg
     5341 cattaacgtc gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta
     5401 ctggtttagc gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt
     5461 actggatgtt gattatcgga ttgcccgcga gctacatcgt aaaaacgtca aactggcgat
     5521 tgatgacttc ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt
     5581 gctgaaaatt gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt
     5641 gaccgatatc atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt
     5701 ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta
     5761 tttgtacgca cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc
     5821 gccgcccgcc cggcataatg gacatatcac gcccattatg ccgttacgtt aatgcttact
     5881 catcttcatc gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat
     5941 tggcttcaat gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa
     6001 tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa
     6061 ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc
     6121 agccgtcacc atcagtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa
     6181 tggcttccag cacatccagc ggcgtgacca gaccttgtac cacaccaaac tcgttggtca
     6241 cgataacaaa gctcccgcga gcacgacgca gcacgcccaa caggttgatc ggatcgaggg
     6301 tttccgggac gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt
     6361 ccagcgccac cagcagttct ttagcacgta caataccgat gatttcatcc agttcaccgc
     6421 gacataccgg gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc
     6481 cgagattagc gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac
     6541 gcgacgccag cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca aatgcacctt
     6601 ccgggatcgg gatcggcatc ggcatcgggt tatcggcatc gtgctgaaca ttggcctgac
     6661 gtttcccgcc catcaaacgc aggatggcat cggcagtacg cgctcgcagc ggcaaagtcg
     6721 actggtggcg aataaagttg cgacgcgcaa tctggttaaa cacttcgatg atgatcgaga
     6781 agccaatcgc ggcatacagg taacctttcg gaatgtggaa accgaaacct tctgccacca
     6841 gactcagacc aatcattaac aggaagctca gacagagcac caccaccgtg gggtgctggt
     6901 taacgaatcg cgtcagcggt ttggatgcca gcaacataac cgccatcgca atcactaccg
     6961 ccgccatcat caccggcaga tggttaacca tccctactgc agtaattacc gcatccaacg
     7021 agaagacggc gtcaaggatg acaatctgtg tgacgaccac ccagaaactg gcgtagcctt
     7081 taccgtggcc ggaatcatga tcgcggtttt ccagccgttc atgcagttcg gttgttgctt
     7141 tgaacagcaa gaatatcccc ccgaacaaca taatcaggtc gcgtccggag aaggagaaat
     7201 ccatgacggt aaatagcggt ttggtcagcg tgaccatcca tgaaatcagc gacagcagcc
     7261 ccagacgcat aatcagcgcc agtgataacc ccagcaaacg cgctttatcg cgttgttttg
     7321 gcggcagttt gtcagcaaga atggcgatga agaccaggtt atcgataccc agcacaattt
     7381 cgagaacaac aagcgtgagt agccccgccc aaattgaggg gtccattaag aattccatga
     7441 caagctcctg cttaaggaat agctattcga cgccagaaat aatgcaggcg taacgacaaa
     7501 atgcaaacga aaggtgcggc atagagagcc agaaaggtag gcgttaaaag gcctgatgct
     7561 gaaatgacgt cggtgacgat ccatactgcg ggctactgcc ctatactcca tggttgttaa
     7621 acgggagtta aacatatcag agacgcctct gatttggcaa agatttacct tcctttgcaa
     7681 acgaatgtga caaggatatt ttacctttcg aaatttctgc taatcgaaag ttaaattacg
     7741 gatcttcatc acataaaata attttttcga tatctaaaat aaatcgcgaa acgcaggggt
     7801 ttttggttgt agcccttatc tgaatcgatt cgattgtgga cgacgattca aaaatacatc
     7861 tggcacgttg aggtgttaac gataataaag gaggtagcaa gtgaccattg ctattgttat
     7921 aggcacacat ggttgggctg cagagcagtt gcttaaaacg gcagaaatgc tgttaggcga
     7981 gcaggaaaac gtcggctgga tcgatttcgt tccaggtgaa aatgccgaaa cgctgattga
     8041 aaagtacaac gctcagttgg caaaactcga caccactaaa ggcgtgctgt ttctcgttga
     8101 tacatgggga ggcagcccgt tcaatgctgc cagccgcatt gtcgtcgaca aagagcatta
     8161 tgaagtcatt gcaggcgtta acattccaat gctcgtggaa acgttaatgg ctcgtgatga
     8221 tgacccaagc tttgatgaac tggttgcgct ggcagtagaa acaggccgtg aaggcgtgaa
     8281 agcactgaaa gccaaaccgg ttgaaaaagc cgcgccagca cccgctgccg cagcaccaaa
     8341 agcggctcca actccggcaa aaccaatggg gccaaacgac tacatggtta ttggccttgc
     8401 gcgtatcgac gaccgtctga ttcacggtca ggtcgccacc cgctggacca aagaaaccaa
     8461 tgtctcccgt attattgttg ttagtgatga agtggctgcg gataccgttc gtaagacact
     8521 gctcacccag gttgcacctc cgggcgtaac agcacacgta gttgatgttg ccaaaatgat
     8581 tcgcgtctac aacaacccga aatatgctgg cgaacgtgta atgctgttat ttaccaaccc
     8641 aacagatgta gagcgtctcg ttgaaggcgg cgtgaaaatc acctctgtta acgtcggtgg
     8701 tatggcattc cgtcagggta aaacccaggt gaataacgcg gtttcggttg atgaaaaaga
     8761 tatcgaggcg ttcaagaaac tgaatgcgcg cggtattgag ctggaagtcc gtaaggtttc
     8821 caccgatccg aaactgaaaa tgatggatct gatcagcaaa atcgataagt aacgtattgt
     8881 gttgattatc actcagtttt cacacttaag tcttacgtaa acaggagaag tacaatggag
     8941 attaccactc ttcaaattgt gctggtattt atcgtagcct gtatcgcagg tatgggatca
     9001 atcctcgatg aatttcagtt tcaccgtccg ctaatcgcgt gtaccctggt gggtatcgtt
     9061 cttggcgata tgaaaaccgg tattattatc ggtggtacgc tggaaatgat cgcgctgggc
     9121 tggatgaaca tcggtgctgc agttgcgcct gacgccgctc tggcttccat catttctact
     9181 attctggtta tcgcaggtca tcagagcatt ggtgcaggta tcgcactggc aatccctctg
     9241 gccgctgcgg gccaggtact gaccatcatc gttcgtacta ttaccgttgc tttccagcac
     9301 gctgcggata aggctgctga taacggcaac ctgacagcga tttcctggat ccacgtttct
     9361 tctctgttcc tgcaagcaat gcgtgtggct attccggccg tcatcgttgc gctgtctgtt
     9421 ggtaccagcg aagtacagaa catgctgaat gcgattccgg aagtggtgac caatggtctg
     9481 aatatcgccg gtggcatgat cgtggtggtt ggttatgcga tggttatcaa catgatgcgt
     9541 gctggctacc tgatgccgtt cttctacctc ggcttcgtaa ccgcagcatt caccaacttt
     9601 aacctggttg ctctgggtgt gattggtact gttatggcag tgctctacat ccaacttagc
     9661 ccgaaataca accgcgtagc cggtgcgcct gctcaggcag ctggtaacaa cgatctcgat
     9721 aacgaactgg actaacaggt gagcgaaatg gttgatacaa ctcaaactac caccgagaaa
     9781 aaactcactc aaagtgatat tcgtggcgtc ttcctgcgtt ctaacctctt ccagggttca
     9841 tggaacttcg aacgtatgca ggcactgggt ttctgcttct ctatggtacc ggcaattcgt
     9901 cgcctctacc ctgagaacaa cgaagctcgt aaacaagcta ttcgccgtca cctggagttc
     9961 tttaacaccc agccgttcgt ggctgcgccg attctcggcg taaccctggc gctggaagaa
    10021 cagcgtgcta atggcgcaga gatcgacgac ggtgctatca acggtatcaa agtcggtttg
    10081 atggggccgc tggctggtgt aggcgacccg atcttctggg gaaccgtacg tccggtattt
    10141 gcagcactgg gtgccggtat cgcgatgagc ggcagcctgt taggtccgct gctgttcttc
    10201 atcctgttta acctggtgcg tctggcaacc cgttactacg gcgtagcgta tggttactcc
    10261 aaaggtatcg atatcgttaa agatatgggt ggtggcttcc tgcaaaaact gacggaaggg
    10321 gcgtctatcc tcggcctgtt tgtcatgggg gcattggtta acaagtggac acatgtcaac
    10381 atcccgctgg ttgtctctcg cattactgac cagacgggca aagaacacgt tactactgtc
    10441 cagactattc tggaccagtt aatgccaggc ctggtaccac tgctgctgac ctttgcttgt
    10501 atgtggctac tgcgcaaaaa agttaacccg ctgtggatca tcgttggctt cttcgtcatc
    10561 ggtatcgctg gttacgcttg cggcctgctg ggactgtaag actgttgtac actaccgggg
    10621 ccttttggcc ccgttttttt atctggagga ttaatgacaa tcacggacct ggtactgatt
    10681 cttttcatcg ccgcactcct ggccttcgcg atctacgatc agttcatcat gccccgccgt
    10741 aatggcccca ccctgctggc aattcctttg ctccggcgtg gtcgcatcga tagcgttatc
    10801 ttcgtcggat tgattgtcat tcttatctat aacaacgtca cgaatcatgg tgcgttaata
    10861 acgacatggt tattaagcgc actggctctg atgggttttt atatattctg gatccgcgtt
    10921 ccgaagatca tctttaaaca aaaaggtttt ttcttcgcca atgtctggat tgaatatagc
    10981 cgaatcaaag cgatgaactt gtcggaagat ggcgtgttgg tgatgcaatt agaacagcgt
    11041 cggctgttaa tccgcgttcg aaatatcgac gatctggaaa aaatttataa gcttctcgtt
    11101 tcaactcaat aagttatgaa tttagccaaa gctatg
//
LOCUS       DAPHJE010000130        11067 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPHJE010000130 DAPHJE010000000
VERSION     DAPHJE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.131"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            854..1060
                     /locus_tag="RH222_003739"
     CDS             854..1060
                     /locus_tag="RH222_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV3798692.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1154..1756
                     /locus_tag="RH222_003740"
     CDS             1154..1756
                     /locus_tag="RH222_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798693.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(2645..4246)
                     /locus_tag="RH222_003741"
     CDS             complement(2645..4246)
                     /locus_tag="RH222_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV3798694.1"
                     /translation="MNDTSSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNLLQQESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPTEPCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACRRC
                     DRIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHIPLYRQSEIYARQGVVLSRSVLSG
                     WVDACCRLLAPLDEALQHYVLTDGKLHADDMPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGHIKEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIESELRGKRAEERQAVRHQKVLPLLASLEG
                     WLREKQKTLSRHSELAKAFGYALNQWPALTRYAEDGWVEVDNNIAENALRLVSLGRKN
                     WLFFGSDHGGERGASLYSLIGTCKLNGVDPERYLHHVLDVIADWPVNRVGALLPWRVT
                     LPDQLHFVNTVLTARLPFSARHMSM"
     gene            complement(4296..4643)
                     /gene="tnpB"
                     /locus_tag="RH222_003742"
     CDS             complement(4296..4643)
                     /gene="tnpB"
                     /locus_tag="RH222_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3798695.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGIDW
                     RQPKRLLTSLTML"
     gene            complement(4640..5023)
                     /locus_tag="RH222_003743"
     CDS             complement(4640..5023)
                     /locus_tag="RH222_003743"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3798696.1"
                     /translation="MRAKERLPRKHYSPEFKMELVRLALEEEGSIAALARKHDVNDNL
                     LFKWIRLWQREGRVCRPRKNSSSLPALIPVQLQAGSSLPTFELPSCSPPATCHIKCRG
                     GEITLTHPSAELMSTVLRELMRGPV"
     gene            5102..5261
                     /locus_tag="RH222_003744"
                     /pseudo
     CDS             5102..5261
                     /locus_tag="RH222_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            5305..5460
                     /locus_tag="RH222_003745"
                     /pseudo
     CDS             5305..5460
                     /locus_tag="RH222_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            6591..7742
                     /locus_tag="RH222_003746"
     CDS             6591..7742
                     /locus_tag="RH222_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798697.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLCAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDQIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            7826..8491
                     /locus_tag="RH222_003747"
                     /pseudo
     CDS             7826..8491
                     /locus_tag="RH222_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            complement(8573..9319)
                     /gene="istB"
                     /locus_tag="RH222_003748"
     CDS             complement(8573..9319)
                     /gene="istB"
                     /locus_tag="RH222_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDV3798698.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            complement(9334..10875)
                     /gene="istA"
                     /locus_tag="RH222_003749"
     CDS             complement(9334..10875)
                     /gene="istA"
                     /locus_tag="RH222_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HDV3798699.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
BASE COUNT         2711 a         2896 c         2799 g         2661 t
ORIGIN      
        1 gcacgcactc gtaaaatttg cggtcagttc cgtcagtcag tgggcaaccg cagagttacg
       61 ggcttccagg tccacaggag tgagtctata acgcgtgtgg gaaatcattt tttctggcgt
      121 tgacagaacg ccttacgggc tgctacttac ccggtgtccc gcaatacagc cttagtgttc
      181 cgttatcggg gatccggacg caccgcaaca accggggaag tatttctggt tagagctgtg
      241 tcaagaggga tttctcagcc cctcactcac cggctcagtc tcagggcaga agcattaata
      301 cactcagttc atgtcttata caggaatggg cataggggga gctggcgtgt cactgctgat
      361 tttacgcatc agcgttcatg tatctgcact ctgatacaga atgcttcaca gcgctctgtt
      421 accggagaga tttactctct taggttattc tggcaaatcc tttacgttac tctctgatga
      481 cttcttatat ttataatacc aatcagacaa ccttatccca tcggtgatat cagtctcctg
      541 ctcgccagga acgggctgac tcactgtaac cacctccgaa aacaactctg caattatcag
      601 aacgcctgct tctctccctg tcctcacgaa aactatcccc tctttatcgc gcgtgcgtgc
      661 ggaagcatct tttcgcaaca cccccctccg ggattccgct acggctctgc catcgcaatc
      721 cccccgttta tctccggaca gccacattcc cgattatttt ttacgtttct ccccggttgt
      781 tatgccggtg aaggtggcgc gctgttttca tgaccacacc ggctggttta acaacatccg
      841 gaggaacatt ctcatggcta ccccagtttc gctgatggat gaccagatgg tcgacatggc
      901 gtttatcact caactgaccg gcctaaccga taagtggttt gacaaactca tcaaagatgg
      961 gggctttcct gcgcccatca aaatggggcg cagctcccgc tggctgaaaa gtgaagtgga
     1021 agcctggctg caggcgcgta ttgcacagtc ccgtccgtaa tttttaacca ctaacccttc
     1081 gcccacagca gacgcaatct ccggcctgcc ggcggcgctc tgctgcctgt tatcccccgt
     1141 gaggaatatg aaaatgaaac aacagtatca gacccgttac gagctgctcc atgaaaacta
     1201 ccagaaatgg ctgaccggct tcacccggca cgccgtatca tggggcgtgt gtcatccgaa
     1261 tatctactat ttccataatc tgacgcccgg gtgggtgtca ttcaatggcg aaaaaccgga
     1321 gattgccatc gttccccaga gtctgcaccg gctgatttat ggtccggaca aacggagctc
     1381 cccgtccctg gatgatgatc tggtggtgaa tttatgcacc agcgaacacc tgctggttca
     1441 tcatccgatg ctggaaggga tcctgctgtc tgaatgtgag cgcctgaagc aacattctct
     1501 ggcaaacaaa ctgatcagcc tcttccgtca gtttggcggc acggagttac gtctcaaact
     1561 ggtctggctc tgctggcttg acttaatgac cggaaacagc cttgacgact ggacgaaaaa
     1621 cctgaaacac aaatcagaaa aagatctgga gcaatggatc attgcacgtc agggtcagag
     1681 tgaaccactg acaaatctga tggatcagta cgtgcttatg gcataccgta catcggttga
     1741 tgccgctcac agttgatgtc tgagaaccac agggctggca gcagaaaacc agccctgtgg
     1801 taaaatacag taatagcggc gtacatcagg cacgccgctc actcgtccac agatcatata
     1861 cgtcattata ctgcgcgata acggagggct tcagcacccg gtcatttctg agcgggtatg
     1921 aataccattg atgatgcgaa gcagccacgg gcagaaacgt tcttcacaac caggtgcctc
     1981 catcttcact gcagtctgaa tgtgccggct tgttatccac cagaaatggt atgctccggt
     2041 acatgcatac cccggagact ataaacgcac tgaatcatag cctcccacct cagcaggagg
     2101 cagtcagctt tcaggcggaa aaacgttgca gttaaagatc gtgagaatcg ctgtttcgtt
     2161 gcatagcaac ccgaacagtt gtagtgaaac attttacctg catcagcaca tggtcaggcg
     2221 ccgtctgtat ggcgtggaat actgatgttt cggctgatgg caagcctcat tctctgtgga
     2281 aacaggggcg cgcattagct tctcaaccag agagtgtcac aaagacaaat attgctgaaa
     2341 atcgcattca tcgttcaggc gtgaatgccc ggagacaaaa gccgctgtcc acacagtccc
     2401 cctctgacct gaacaaaaag catacacaca acaggctcac ctgaccccga aaaaccataa
     2461 cttcagcaat accagtgaca ccagcacgtt acctcagtgc cacatcttca gaccgcgatc
     2521 aatctgaaat gtgactatcg gggcaatatc cctgctttgt ggtacacgat aaccagaaaa
     2581 catcccctga accgccagcc agaaagactg gccacaaaag gggtaagctg aaatcgttta
     2641 tggattacat cgacatatgc ctggcagaga atggtaagcg tgcagtgaga accgtattga
     2701 cgaagtgaag ctggtcaggc agagtaaccc gccacgggag cagagcaccc acccggttca
     2761 caggccagtc ggcgatgaca tcaagtacat gatgcagata gcgttccgga tccacgccgt
     2821 ttaatttgca cgtcccgata agactgtaca gtgacgcacc gcgctcacca ccatgatccg
     2881 agccgaagaa cagccagttt tttctcccca gactgaccag tcgtagggcg ttttcggcaa
     2941 tgttattatc cacctccacc cagccatctt ctgcatagcg ggtcagcgcc ggccactggt
     3001 tcagcgcata cccgaacgcc ttcgccagtt ctgagtgcct tgagagggtt ttctgtttct
     3061 cccgcagcca cccctccagt gacgccagca gcggcagcac tttttggtgc cggactgcct
     3121 gccgttcctc tgctctttta cctctgagct ccgactcgat ggcatacagc tcgccgatcc
     3181 gtttcagcgc ctcctccgtc agggctgacg gggtacggac gtgaacatcg tggatttttc
     3241 ggcgggcatg ggcccagcac gcggcttcct ttatatggcc gtcgcggtag agctcgttga
     3301 acccggcgta ggcatcagcc tgcagtacgc cactgaagcc tgcaagatgg gtctgagggt
     3361 ggataccttt tctgtccggg ctgtaagcga accacactgc gggggccagc gctgatccgg
     3421 cgttgcggtc gtctcgaacg tacgtccata agcgcccggt cttcgtcttc ttattgcccg
     3481 gcaacagcac cgggacaggc atatcgtcag catggagttt gccgtcggtc aggacatagt
     3541 gctgaagggc ttcatccagc ggtgccagca gacgacaaca cgcatccacc cagcccgaca
     3601 gtacagaacg actcaacact acaccctggc gggcatagat ctccgactgg cgatacagcg
     3661 gtatgtgctc tgcatacttt gaggtcagca ctcgggccag cagccccggt ccggcgatcc
     3721 cccgttcgat ggggcgcgaa ggagccgggg cctgaacgat gcgatcgcac cgacggcagg
     3781 catgcttttc acggactgtc cggataaccc ggaaggcgct gcgcatcagc tccagctgtt
     3841 cggcggcatc ctcacccagg taactcagcg aaccaccgca ctccgggcaa cacggctcag
     3901 tcggcagcag ccgcttctca tcccgtggga gtgactcagg gaacggtttg cgggtgcggg
     3961 tctgacgcag cgggcgctgc actgccgggt catccacccg gccggtcagc gtatcgcttt
     4021 cctgctgcag caggttcagg tcagcttcca tttgcgcgat acggcgggat accttttcgg
     4081 aacgactgcc gaagttcatc cggcgcagct tatccagttg cgcctgcaga tggtctattt
     4141 cgcgttcccg gttgctcagc ttttcctgca gggcgtggat caacgcttcc tgttcggcca
     4201 ggcgctgttt cagcagaagg atgtcgtcag aagaggtgtc gttcataagc ctgtatttta
     4261 ccaggcttat tctgcgacaa ccaggataaa gagcgctaca acatggtcag ggatgtcagt
     4321 aaccgctttg gctgtcgcca gtcgatacct tccatcagca tcgccagttg cgccggcgtc
     4381 aggaacactt tgccatcgcg ggctgagggc caggcgaagc gaccacgttc tagtcgcttt
     4441 gtcagcaggc acagaccatc gccggtggac cagagcagtt ttacctgact gccgctgcgt
     4501 ccccggaaga tgaagacgtg gccggacatc gggtcatctt tcagcgcggt ctgcaccttt
     4561 gcggcgaggc cattgaagcc gttgcgcata tcggtgatac cggcaaccag ccagattttt
     4621 gtgcctgcgg gaagatttat catacaggcc cccgcatcag ttcgcgcagg acagtgctca
     4681 tgagttcagc agagggatga gtcagtgtta tttctcctcc ccgacatttt atgtggcagg
     4741 tagcgggagg agagcaggat ggtagttcga aggttgggag agaagagccc gcctgaagct
     4801 gcacgggtat cagggcagga agcgacgatg agttttttcg gggccgacag acccgccctt
     4861 cacgctgcca gagccttatc catttaaaga gcaggttatc attgacgtca tgtttccggg
     4921 ccagtgcggc aatactgcct tcttcttcaa gagccagcct gaccagttcc attttgaatt
     4981 caggggaata gtgtttccgg ggaagtcttt ctttagcgcg catgaggtgt ccacctgcaa
     5041 aataagtggg tgcttaagtt acaggatcct ggattcggga ccagacggta ctcagacgac
     5101 gcttacagag aatgtccgga atgacgaggt tatctttgcg gagattaagg caatggtata
     5161 cgggccgacg ttccatgccg cgttactaca ttcagcccgg acgtaaaatg cgcccgtcaa
     5221 aaagaacggt tcgcttcgaa actcagccag gataccacct ctccgccaca cccgggaaga
     5281 agttgatatg gtggttgccg gacaacagcg ctcctggatt gactgctgca tcactcaacc
     5341 acgctgaata tcaaaggaga gcgttaccgg ttaaaagaga aacgtaaagc tgtggtgatg
     5401 acccaaaacg caacgcctgt cagtgaggat gaaatggtga aaagcggaca gcatcagtaa
     5461 cggaagtatc ttagcgggta cgaaaatggc aggatcgtga cattgacaga atatctcctg
     5521 atcatcctga atgactgcag ttgctgtaga aaagtaagct gtctccaccc acggcaccac
     5581 ttaacaggca ttccgtaact tcccccagac ttactatgac gcattatcgg tgcactgaaa
     5641 acgggagagt gcgcttaaaa ccgcagaccg catgcgggtg ttactgcatg acagtctggc
     5701 cgtattgtga gatgtcggca ggaaccgatg tccgcgcagg ggggaacttc ctgcagtacc
     5761 tgacgcttca aatggtatta accgtagggt gttgttggtt ccggatcgcg gaaatgggag
     5821 cacaggtctg ctacggggtg tacctctgac agtatatccg gctcgctcat ctgacagcct
     5881 ctctcccgga aaagaccaaa aatgagatcg tctcctcaac agcagagaag aggtcttgac
     5941 agacaggcta tattgcatac gatttatgaa gaaaaagaca gcaaatatgg aggttatagc
     6001 ggagaatacc ctacgaaatt cctatgaaaa acgattgaaa aaaatatcaa attcgattcg
     6061 tttttatatg ctttttggga gcgtggcgat gacgaactgt tgcatgtgca gaggagaatg
     6121 cacccggaag caccagaatc atccagaaag gcagaacgat tacgcatcaa accaggagac
     6181 agcaatatca aggtaatata ctgatttaaa atgaatttac agcaatacgt gaaaacattt
     6241 tttacttaaa ggagacagat gcgctgaaga gagtgcaggt atttttatat aaaaatgaga
     6301 agtttacggg atgtatatcc ggcacactat gaccggagaa tatcagtcaa gcttaaaaag
     6361 acaataaata cagtgagtta aatacacaga gaaaacgtag tatcagaggg tacatcttac
     6421 aacagagact gaatttccgg tttttcataa ccggcgcatt ccggaaagtg caggcggccc
     6481 ggaggttttc tgacgccact acctggtaga ttgttctgta catcatgtac ggaggttttt
     6541 gtttgctttt ttaaaatctg caaatgtgag aaaagcaggt gaggtgtatt gtgggaaagg
     6601 aactggatga tatctgcacc agctgtccgt atatcgatgc tgtaaaacgg cacaaacaac
     6661 agctctgtgc catagaggaa tatacacagt ggttaaaaaa agaaccacgg gcatcttatt
     6721 tttttctgtt ccagctgtat acccgcattc acaatacatt tttctcccaa aagcaacaac
     6781 tcccgttcac tcccggtggc actcactgcc cggaacctga tgtcaccctt cgggacttaa
     6841 cgctttcccc tggctaccac agtgattatg cccctcaacc cattccggag atggacagtt
     6901 ctgctgttgt tcccccaact aatgaaaaca cctcgcctcc ggaagatact cccgacaaca
     6961 ctccggccgg aggcaatacc ggacaagcgg aaaagaccag aaacgcagga ctcacaccca
     7021 ttcctgaaaa acgttcagga atgcccccgg aacatctgcg gtttgccacg gggtttccat
     7081 cgcaaccgaa gattgccggt cccagagaaa agccgatgcg caccgtgcat ccggatcaaa
     7141 tctaccgtga aattatatgg ttctgctcca gttacctgct gaaatccgga ccagaggcta
     7201 cgaggacgat tatcaactca gtattctctg aatgggccag cataaacaat gattacccct
     7261 cccccttttc gtgggtggac agcagggaca gtgaacagtg tgactggtta tggaacgcca
     7321 tgcaggtccg gtgtgtggga accccgctga atccccttac cccggagcag aaatactggt
     7381 ttgcctgcgc cacgtttgat aactgggagg gctggaatga gcaacaggta cagtttttac
     7441 tggaaagtaa tcccagacgg aacagagcga agtttacgca ggtctccttc caggccccca
     7501 ggattcagca taaagcgatt cttcttgatg agctgaaaag tgcccgggag caacaaaaaa
     7561 ggcgtgatga acgtgctgat ggttccgtcc ctctgaaact gtccggaaaa atccacaaac
     7621 agcttgaaag tattgcccga agtcgtggtg tcctcccaaa aaaactgctg aatgaaatga
     7681 ttgagcaggc gtaccatgat ttggtggcaa ccaggcagaa cagccaaatc gattccagat
     7741 aatttcagac agatattatt tccgtgaatc ccccgccacc tttccggtgc gcggggtttt
     7801 gtcttttttc accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa
     7861 accgctctcc tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact
     7921 caccagtcac gaacccgtgc ctggcattat gggtaaaagc ggggccggta aatcctcact
     7981 ctgtaatgcg ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcccccg
     8041 ggaagtgcag cgcttccgtc tgaatggcca tggtcacagc atggttatca ctgacctgcc
     8101 cggggtgggc gagagccggg acagggatgc aaagtatgaa gccctgtacc gtgacattct
     8161 gctcgaactg gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga
     8221 tgagtatttc tggcgacaca tcctgcaccg gggacatcag cgggtactgt ttgtggtgat
     8281 gcaggccgat aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc
     8341 cgcagaggca cagaacattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt
     8401 acatcgggtt gttgccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc
     8461 gctcatgaca gcacttcccg accatgctgc gtattaccgt gaaggagatc ggtgagtaac
     8521 atcgatggag atcggttcgt gtcactttca cagaaccgtt tttaaattac cttcactgat
     8581 ctccttcggt caacggagat tgtattttcc gcattgattc tcctttaagt tcgatcttga
     8641 tactgccatg aaccagtcga tctaggatgg catccgcatg tgtggagtct ccgatcattt
     8701 tgtaccagtt ttccaccggc aactggctca ctacgatgat tgagcctcgt tggtacatca
     8761 gatccactat ttccagcagg tcgctacgct gttctgatga gagaggttcc agcccccagt
     8821 catccagaag cagcagatcg ctattattca gcctggtcaa aagtttgctg tatcttccat
     8881 cagcatgccc ctgatagcac tgttccatca gcgctttaag gcgataatag tagaccttgt
     8941 atccctgtcg gcaggcatta tgaccaagtg cacatgccag gaacgtttta ccgctgccgg
     9001 tggccccggt cagtaaaata ttttgtttca gggttaacca gtttccctga ctgagtgaac
     9061 ggatgagggc cctgtccagc cctctattgt tacgataatc cagctttgat aactcagcat
     9121 taagtctgaa ccgtgcatgt ttgatcagac gctctgcctt cctgttttca cggcaggtta
     9181 gttcttctgc tgtcagtaat gacaggcgtt cttcgaagcc cagctcctgg tatgtgcccg
     9241 gctgagcaag ttgcttttta agcgcatcac ggaagccggt gagtttaagt gcggtcagtt
     9301 gttcgtaaag atgattcatc attggatccc gtatcagtgg taatactcac tgccgcgtac
     9361 gttttcgtgt tccatcgtgg ataacagatc tggttttgga tcctgaacag gttgtttatc
     9421 cagacctttt tccaggatcg atttaatacc tgacagacgc catacctttg ttttcagagc
     9481 tcttgcacat gctgcattaa gtctggcttt actgtatttt ttatgaaggt tcaggagtcc
     9541 aaggcagaag cgatagcttt gttccggatg tggacgagag ttcagtatat gaagcacata
     9601 actatgagtt tcactgccta tgtgccccgc ccattccaga agacgctctg gcgtccaggt
     9661 ggcatgctgt cgatgagctt caggcatgtg ctcgttgcgg gtactgtagc cataagtacg
     9721 cttgcgcggg tgcacagcaa cctcctgccc ctgattgaag agtcttacca gttctccgga
     9781 gatccatgct tccagttgct ggcctaacag cgaacatgga accgagtagt aatgtttgtc
     9841 gatttccacg tggtaatcgg catgaactct gactttcttc accagggtgt aactgtaact
     9901 ggcttcagga agaggcttca gtgcgggttt atcaagctgg atgaagagtt ctgcacgtga
     9961 ataacccaac ttctgcatta ttttgttatt cagtctttcc agcaactccc gaatgcgctg
    10021 attaagcgat gcaaggctgt agaagatctc atgcctgatt cgggccatga tccagcgttc
    10081 aacaacctga acgccaactt cagctttggc tttatctttc ggtttacgtg gccgcgcagg
    10141 caaaactgcg acattataat gctcaagcat ctgctggtag gtagggttaa cgtcaggatc
    10201 atacttacat gccctggatg tggcgctttt cagattgtcc ggaacaacaa gttcaggaac
    10261 gccacccaac cactggaagc agcgaacatg actcatcacc cagtcttcaa gctgctgaga
    10321 ccaggtggcc tctgcccatg tgtaacttga tgccccgaga acagctacga tgacctgagc
    10381 agttcttatt tctccggtct cagggtcggt aacgccaacg gtaggtccac agtaatcaac
    10441 gaaaagtttt tcgccagctt tatgtacctg acgcattgat ggtgaagtgg ttttgagcca
    10501 ttcacggtac atccggcagt aatggttata gctgtaaaaa ccgcctggat tacgctcaca
    10561 gtattcttcc cagagtagct gcagcgtcac gcatttatta cgcagttccc ggtgtactgt
    10621 agcccagtca ggcagagagt gcttcttcat cttaacctgg gtctgaagga acgcatgttt
    10681 tagttttgta tcatcccatc ctgtaggtaa gggccactgc tttatgccaa gttgagccgc
    10741 ccgattagca tatctggata caacggaagg ggagattgca agactacgac caatttgtcg
    10801 atggctgagt ccaacaccgt atttaagcct aagaatttct ttaagttttc tcatagaaat
    10861 tggaactgtt ggcataggta tcctttaccg gaatggcaaa agatacagat caacacacct
    10921 gtgaagttcc aataacattg atggagatca ctgaataaca aaatgagtca aaagtgatct
    10981 ccatcgatgt tattcagcga tctgttcaaa tgttattacc cgatctcgat ggaagttatt
    11041 gagtgatctc ctttcatgaa aatacgc
//
LOCUS       DAPHJE010000131        10993 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPHJE010000131 DAPHJE010000000
VERSION     DAPHJE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.132"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            181..1395
                     /locus_tag="RH222_003750"
     CDS             181..1395
                     /locus_tag="RH222_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDV3798700.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1462..2745
                     /locus_tag="RH222_003751"
     CDS             1462..2745
                     /locus_tag="RH222_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3798701.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2997..3914
                     /gene="oxyR"
                     /locus_tag="RH222_003752"
     CDS             2997..3914
                     /gene="oxyR"
                     /locus_tag="RH222_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV3798702.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3897..5297)
                     /gene="sthA"
                     /locus_tag="RH222_003753"
     CDS             complement(3897..5297)
                     /gene="sthA"
                     /locus_tag="RH222_003753"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV3798703.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5628..6278
                     /gene="fabR"
                     /locus_tag="RH222_003754"
     CDS             5628..6278
                     /gene="fabR"
                     /locus_tag="RH222_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV3798704.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6278..6637
                     /locus_tag="RH222_003755"
     CDS             6278..6637
                     /locus_tag="RH222_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV3798705.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6677..7777)
                     /gene="trmA"
                     /locus_tag="RH222_003756"
     CDS             complement(6677..7777)
                     /gene="trmA"
                     /locus_tag="RH222_003756"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV3798706.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7891..8081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8146..9990
                     /gene="btuB"
                     /locus_tag="RH222_003757"
     CDS             8146..9990
                     /gene="btuB"
                     /locus_tag="RH222_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV3798707.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9935..10792
                     /gene="murI"
                     /locus_tag="RH222_003758"
     CDS             9935..10792
                     /gene="murI"
                     /locus_tag="RH222_003758"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV3798708.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2531 a         2605 c         2940 g         2917 t
ORIGIN      
        1 cgtgttgttt taacgatggt ggttaattat tcaggtggtt tttgagggta ttttttctcc
       61 tcggcggaaa tttttatttt taatgtgatc acggttgaat aatcgctctt tctctcttgt
      121 atggtagtag gcgtaaaatt aagtttgaag cgtctattta caacacagga gattgcaata
      181 atgaaaatta ttgctgcaga tgtttttgtt acttgcccag ggcgtaattt cgttacgctt
      241 aaaataacca ctgaaagcgg actttgtgga ttaggagatg ccacgttaaa tggtcgcgaa
      301 ttgtcggtcg cttcttatct gaaagatcat ctttgtccac aactaattgg ccgcgatgcc
      361 agccgtatcg aagatatctg gcaattcttt tataaaggtg cttactggcg acgtggcccc
      421 gtcacgatgt cagccatttc tgccattgat atggcgttgt gggatatcaa agctaaagcg
      481 gcaaatatgc cattgtatca actgcttggt ggtgcttctc gtgaaggcgt gatggtttat
      541 tgccacacca ccgggcggac gattgatgaa gtccttgaag actatgcaaa gcaccagcag
      601 atgggcttta aggcgattcg tgtgcagtgc ggtgtaccgg gtatgcaaac tacttatggc
      661 ctggcgaagg gcaaaggcct ggcgtatgaa ccggcaacca aaggcctgtg gccagaagag
      721 cagttgtggt cttcagaaaa gtatctcgat tttacgccga agttatttga agcggtgcgt
      781 aataagttcg gttttaacga acatctgttg catgacatgc accatcgcct gacccccatc
      841 gaagcggcgc gctttgggaa gagcattgag gattaccgcc tgttctggat ggaagatccg
      901 acgcctgcag agaaccagga atgttttcgt ctgattcgcc agcataccgt gacgccgatt
      961 gcggtcggtg aagtgtttaa cagtatctgg gactgcaagc agttaatcga agagcagctg
     1021 attgactata ttcgcacgac gatcacccat gcaggcggca ttaccgggat gcggcggatt
     1081 gccgattttg catcgcttta tcaggtacgt accggttcgc atggcccttc agatttatcg
     1141 cctgtttgtc atgcggcggc gctgcatttt gacctatggg taccaaattt tggcgttcag
     1201 gagtacatgg gctattccga acaaatgctt gaagttttcc cgcacagctg gcgcttcgag
     1261 gaaggttata tgcacccagg cgatgaaccg gggctgggta tttctttcga tgagaaactg
     1321 gcggcgaaat atccgtatga cccggcgtat ctccccgtcg cacgactgga agatggcaca
     1381 ttgtggaact ggtaataacg gttcgcttaa ttattttctg aatagtcatc aaatcgatta
     1441 attccgctca aggagctaaa aatggacgtt gatgtgtcaa ccagtgttgc gggtaataaa
     1501 ccgcaacgga ttcgtcgtat tcagacagtg accctggttt tattatttat ggcggggatc
     1561 gttaattttc tcgaccgttc gtcattgagc gtggcaggtg aagcgattcg tggcgagtta
     1621 ggattatcgg cgacagagtt tggtgttttg ctttctgcat tttctctgtc ttatggtttt
     1681 tcacaactac cttccggtat tttgctagac cgctttggcc cacgaattgt tttaggcgca
     1741 ggcttaatat tctggtcatt aatgcaggca ttaacaggaa tggttaattc ttttagccac
     1801 tttattttaa tgcgtatcgg tctgggtatt ggcgaagcgc catttatgcc tgcgggagta
     1861 aagtcgatca ccgactggta tgcacaaaaa gaacgcggca cggcgctggg gatttttaac
     1921 tcatctaccg ttatcggtca ggctatcgcg cctcctgctc ttgtattgat gcagctggca
     1981 tggggctggc ggacgatgtt cgttatcatc ggcgtggcag ggattctggt tgggatctgt
     2041 tggtacgcgt ggtatcgcaa ccgggcgcag tttgtcctga ctgacgaaga acggacgtat
     2101 ctctccgcgc cggttaaacc gcgtccacag ctgcaattta gcgagtggct ggcgctgttt
     2161 aagcatcgga caacctgggg gatgatttta ggtttctctg gcgtcaatta taccggttgg
     2221 ctctacatcg cgtggttacc cggttatttg caagccgagc aaggtttcag tctggcgaaa
     2281 accggttggg tggcggcgat tcctttcctc gcggcggcag tcgggatgtg ggttaacggt
     2341 attgttgtcg atcgactggc gaaaaaaggc tacgacctgg cgaagacgcg taaaacggct
     2401 attgtctgcg gtttgatgat gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg
     2461 ccagcccagg cggtagcgtt tatctcaatg gcgctattct gcgtgcattt cgctggaacg
     2521 tctgcatggg ggctggtgca ggtgatggtg tcagaaacaa aagtggcttc catcgccggg
     2581 attcaaaact ttggcagttt tgtttttgct tcctttgctc cgatcgtaac cggttgggta
     2641 gtggatacca cacactcgtt taatctggcg ttggttatcg cggcctgtgt gacgttcacc
     2701 ggggcgctgt gttacttctt tattgtcaaa gatcgcattg agtaatcgtt actatcaggc
     2761 tctcttgctg tgggcctgta gaataaaaaa aagcggatcc tggagatccg caaaagttca
     2821 cgttggcttt agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta
     2881 aaagaggtgc cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat
     2941 cgttcattgc tattctacct atcgccatga actatcgtgg cgatggagga tggataatga
     3001 atattcgtga tcttgagtac ctggtggcat tggccgagca ccgccatttt cggcgtgcgg
     3061 cagattcctg ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg
     3121 agttgggcgt gatgttgctg gagcggacca gccgtaaagt gttgttcacc caggcgggaa
     3181 tgctgctggt agatcaggcg cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg
     3241 caagccagca gggcgagacg atgtccgggc cgctgcacat tggtttgatt cccacagttg
     3301 gaccgtacct gctaccgcat attatcccta tgctgcacca gacctttcca aagctggaaa
     3361 tgtatctgca tgaagcacag acccaccagt tactggcgca actggacagc ggcaaactcg
     3421 attgcgtgat cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg
     3481 atgagccaat gttgctggct atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac
     3541 cgatggccga tctggcaggg gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg
     3601 atcaggcaat gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga
     3661 ccagcctgga gacgctgcgc aacatggtgg cggcaggtag cgggatcact ttactgccag
     3721 cgctggctgt gccgccggag cgcaaacgcg atggggttgt ttatctgccg tgcattaagc
     3781 cggaaccacg ccgcactatt ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct
     3841 atgagcagct ggcagaggcc atccgagcaa gaatggatgg ccatttcgat aaagtgttaa
     3901 aacaggcggt ttaaaccgtt taacgcagct acccgatagg cttccgccat ggtcgggtag
     3961 ttaaaggtgg tgttgacgaa gtactcaata gtgttgccgc cacctttctg ttccataatc
     4021 gcctgaccga tatgaataat ttcggcagcg cgctcgccaa agcagtgaat acccagaatc
     4081 tcttttgttt cccgatggaa caaaattttc agcgtgccca cgttcatgcc gacgatttgt
     4141 gcgcgtgcca gatgtttaaa ctgggcgcgg cccacttcat atggcacttt catcgcggtc
     4201 agctgctgtt cggttttgcc cacagagctg atttccggga tggtgtaaat gccggtaggg
     4261 atatcttcaa tcagatgcgc ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc
     4321 ccctggtcat aggccgctga tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa
     4381 acgtgcggct gtgcggtctg atacatgctg ttgaccttca gctgtccgcg actgtcagtt
     4441 tccagcccaa tgttctgtaa cgccagcgaa tcggtattac cggtacgacc gttggcatag
     4501 agcaggcagt cagctttcag ttttttaccc gacttcagat gcatgatcac accgtcgtca
     4561 cagccttcga tcttctcgta ctcttcgttg tgacgaatca ctacgccact gttccagaag
     4621 tgataggaga gagaatctga catctcttga tcgagaaatg ccagcaggcg atcgcgggtg
     4681 ttgatcagat ccacttttac atccataccg cggaagatcg acgcatattc acagccgatc
     4741 actccagcac catagataag tacatggcgc ggttcgtggt gcatgctaag aattgagtcg
     4801 ctgtcgtaaa tgcgtggatg ggtgaaatca acatctgttg gatgatatgg acgagagccg
     4861 caggcaataa caaatttttc agcggttagt gtttcaacgc tgccgtccgg gcaatccagc
     4921 gccaacgtat gctcgtcaac aaagcgagcg tttccctgca atatttcaca gtgattacgt
     4981 tcgtaaaatc cctgacgcat gcgcgtttgt tgattaatca cgttatcggc atggttaagg
     5041 atatcggcaa aagaagagcg gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg
     5101 aattctataa tgcggctgac ggcgtgacgg agagctttcg acgggatggt gccccagtgg
     5161 gtgcaaccgc cgccaacatt ttgataacgc tcgataactg cgacgcgcgc accttgctta
     5221 accaggccca ttgcagcgcc ttcgccgccg gggccggaac ctattactat ggcatcgtaa
     5281 tcgtaggaat gtggcatggt agggcttacc tgttcttata cataaaagca acagaatggt
     5341 aacattttat cgcgggtaag ccaattgatc cccgtcattt atctggctat atcctgagcg
     5401 gcctttgctt tgtctgtttc ttacttttgc cctgacgttt tattggattt ttatcgacga
     5461 tactctccgt ttaagcggca ggtttccgct gtacgtaaaa gaaccggcca aagaattgca
     5521 gtaaatatgt tttattgcgt taccgttcat tcacaatact ggagcaatcc agtatgttca
     5581 ttctctggta tagtgccagc agtacttttg gcaaggattc agacatcgtg atgggcgtaa
     5641 gagcgcaaca aaaagaaaaa acccgccgtt cgctggtgga agccgcattt agccaattaa
     5701 gtgctgaacg cagcttcgcc agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg
     5761 ctcccacctc tttttatcgg catttccgcg acgtagacga actgggtctg accatggttg
     5821 atgagagcgg tttaatgcta cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag
     5881 gcgggagtgt gatccgcacc tcggtctcca catttatgga gttcatcggt aataatccta
     5941 acgccttccg gttattattg cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg
     6001 ttgcgcgtga aattcagcac ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc
     6061 atatgccgcg tgcgtttact gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg
     6121 cgggtgccga ggcgttggac gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg
     6181 tactgcaact gcgaatgatt tcgaaagggg cttattactg gtatcgccgt gaacaagaga
     6241 aaaccgcaat tattccggga aatgtgaagg acgagtaatg aaacaagcaa atcaagatag
     6301 aggtacgctg ctgctggcgt tggttgctgg cttatcgatt aatggtactt tcgcagcgct
     6361 gtttagctcc attgtgccat tttctgtatt cccgattatt tccctggtgc tgacggttta
     6421 ctgcctgcat caacgttatc ttaatcgcac catgccggta ggcttgccgg gtctggcagc
     6481 tgcctgtttt attctcggcg tactgctgta cagcacggta gttcgtgcgg aatatccgga
     6541 tatcggctct aacttcttcc cggcagtact ctccgtcatt atggtgttct ggattggcgc
     6601 gaagatgcgt aaccgtaagc aggaagttgc tgagtaatcg gtattatgcc ggatgctggc
     6661 gcatccggca tgggttttac ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt
     6721 aggggaactg atcaaacaga gccagacgtt cgaccttgtg cgtctggctt aatgtttcca
     6781 gattcttgca taacgtttcc ggattgcagg agatatacag aatacgtgga tacgcctgca
     6841 ccattttctc ggtttcactg tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc
     6901 actgataact cttcaagtct atcccttgca ggcgattaaa ctcgcgtaca ccattcatcg
     6961 cctgagtaaa ttcttctgcc gccatacgaa taatttgtac gttatcaata tggttagctg
     7021 cgatgttgta ttgcgcagca gcaaccgacg gcttagcgat ttcggtggct aatacccgat
     7081 caaaattgcg cgccagtgct aatgaaaagt taccgttgcc gcagtacagc tccagtaaat
     7141 cgccttttga gcctttggtt acgtccagcg cccattccag catctgaata ttcatcgccg
     7201 cgttcggctg ggtaaaactg ttttctacct gacggtagat catctctttc cctgcgaccg
     7261 gcagacgttc atcgatgtaa tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa
     7321 tcagatgcac attcagattc tgcgcgcgca gtgcatcgcg cagggcttcc gcttcctgac
     7381 gccactcatc atccagcttt ttatggtaca acagggaaac caccgcctga ttactcagcg
     7441 tggtgaggta atcaatctgg aacaacttgt ggcgcagaac gggattatta cgcacacccg
     7501 caatcatcgc cgtcatcaac tggttgatga gttcactggc agcggggaag ctatccacgc
     7561 ggatgcggct tttggtttgt tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc
     7621 agatgcggaa ctccgcacgc atccggtaat gactgaccgg cgagcgaaac acttccggaa
     7681 ccaggtcaga aaacggtgcc atcatacttt gcaaacgtac cactttttcg gctaactgcg
     7741 cttcatactg ttctgttgga aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt
     7801 acgcggcgat tgtagggatt gctcatcaga tgtccagatc ttgatgaatt cctatttgtg
     7861 agctacgtct ggacagtaac ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt
     7921 taatagggaa tccagtgcga atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga
     7981 ttgcgttatg cggacactgc catccggtgg gaagtcatca tctcttagta tcttagatac
     8041 ccctccaagc ccgaagacct gccggccaac gtcgcatctg gttctcatca tcgcgtaata
     8101 ttgatgaaac ctgcggcatc cttcttctat tgtggatgct ttacaatgat taaaaaagct
     8161 tcgctgctga cggcgtgttc tgtcacagcc ttttccgctt gggcacagga taccagcccg
     8221 gatactctcg tcgttactgc taaccgtttt gaacagccgc gcagcactgt gcttgcacca
     8281 accaccgttg tgacccgtca ggatatcgac cgctggcagt cgacctcggt caatgatgtg
     8341 ctgcgccgtc ttccgggcgt cgatatcacc caaaacggcg gttcaggtca gctctcatct
     8401 atttttattc gcggtacaaa tgccagtcat gtgttggtgt taattgatgg cgtacgcctg
     8461 aatctggcgg gggtgagtgg ttctgccgac cttagccagt tccctattgc gcttgtccag
     8521 cgtgttgaat atatccgtgg gccgcgctcc gctgtttatg gttccgatgc aataggcggg
     8581 gtggtgaata tcatcacgac gcgcgatgaa cccggaacgg aaatttcagc agggtgggga
     8641 agcaatagtt atcagaacta tgatgtctct acgcagcaac aactggggga taagacacgg
     8701 gtaacgctgt tgggcgatta tgcccatact catggttatg atgttgttgc ctatggtaat
     8761 accggaacgc aagcgcagac agataacgat ggttttttaa gtaaaacgct ttatggcgcg
     8821 ctggagcata actttactga tgcctggagc ggctttgtgc gcggctatgg ctatgataac
     8881 cgtaccaatt atgacgcgta ttattctccc ggttcaccgt tgctcgatac ccgtaaactc
     8941 tatagccaaa gttgggacgc cgggctgcgc tataacggcg aactgattaa atcacaactc
     9001 attaccagct atagccatag caaagattac aactacgatc cccattttgg tcgttatgat
     9061 tcgtcggcga cgctcgatga gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc
     9121 gttggtcacg gtagtattgg tgcgggtgtc gactggcaga aacagactac gacgccgggt
     9181 acaggttatg ttgagaatgg atatgatcaa cgtaataccg gcatctatct gaccgggctg
     9241 caacaagtcg gcgattttac ctttgaaggc gcagcacgca gtgacgataa ctcacagttt
     9301 ggtcgtcatg gaacctggca aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc
     9361 attgcttcct acgggacatc ttataaggca ccaaatctgg ggcaactgta tggcttctac
     9421 ggaaatccga atctggaccc ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta
     9481 accgctgggg tgaactggcg tatttccgga tatcgtaacg atgtcagtga cttgatcgat
     9541 tatgatgatc acaccctgaa atattacaac gaagggaaag cgcggattaa gggcgtcgag
     9601 gcgaccgcca attttgatac cggaccactg acgcatactg tgagttatga ttatgtcgat
     9661 gcgcgcaatg cgattaccga cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac
     9721 cagctcgact ggcagttgta tgacttcgac tggggtatta cttatcagta tttaggcact
     9781 cgctatgata aggattactc atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc
     9841 ttgtgggatc ttgcggttgc gtatccggtc acctctcacc tgacagttcg tggtaaaata
     9901 gccaacctgt tcgacaaaga ttatgagaca gtctatggct accaaactgc aggacgggaa
     9961 tacaccttgt ctggcagcta caccttctga accacgtccc accgtgctgg tgtttgactc
    10021 cggcgtcggt gggttgtcgg tctatgacga gatccggcat ctcttaccgg atctccatta
    10081 catttatgct ttcgataacg tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt
    10141 tgagcgagtg gtggcaattg tcaccgcagt gcaagaacgt tatccccttg cgctggctgt
    10201 ggtcgcttgc aacaccgcca gtaccgtttc acttcctgca ttacgcgaaa agttcgactt
    10261 cccggttgtt ggtgtcgtgc cggcgattaa acctgctgca cgtctgacgg caaatggcat
    10321 tgtcggatta ctggcaaccc gcggaactgt taaacgttct tatactcatg agctgatcgc
    10381 gcgtttcgct aatgaatgcc agatagaaat gctgggctcg gcagagatgg ttgagttggc
    10441 tgaagcgaag ctacatggcg aagatgtttc tctggatgca ctaaaacgta tcctacgccc
    10501 gtggttaaga atgaaagagc cgccagatac cgttgtattg ggttgcaccc atttccctct
    10561 actacaagaa gaactcttac aggtgctgcc agagggaacc cggctggtgg attctggcgc
    10621 agcgattgct cgcagaacgg cctggctgtt agaacatgaa gctccggatg caaaatctgc
    10681 cgatgcgaat attgcctttt gtatggcaat gacgccagaa gctgaacaat tattgcccgt
    10741 tttacagcgt tacggcttcg aaacgctcga aaaactggca gttttaggct gatttggttg
    10801 aatgttgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
    10861 ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct
    10921 cctgagaact ccgacagaga aagcaaaaat aaatgcttga ctctgtagcg ggaaagcgta
    10981 ttatgcacac ccc
//
LOCUS       DAPHJE010000132        10973 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPHJE010000132 DAPHJE010000000
VERSION     DAPHJE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.133"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..734
                     /gene="evgA"
                     /locus_tag="RH222_003759"
     CDS             120..734
                     /gene="evgA"
                     /locus_tag="RH222_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV3798709.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            739..4332
                     /gene="evgS"
                     /locus_tag="RH222_003760"
     CDS             739..4332
                     /gene="evgS"
                     /locus_tag="RH222_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV3798710.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4388..5533)
                     /gene="yfdE"
                     /locus_tag="RH222_003761"
     CDS             complement(4388..5533)
                     /gene="yfdE"
                     /locus_tag="RH222_003761"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV3798711.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5607..6551)
                     /gene="yfdV"
                     /locus_tag="RH222_003762"
     CDS             complement(5607..6551)
                     /gene="yfdV"
                     /locus_tag="RH222_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV3798712.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6621..8315)
                     /gene="oxc"
                     /locus_tag="RH222_003763"
     CDS             complement(6621..8315)
                     /gene="oxc"
                     /locus_tag="RH222_003763"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV3798713.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKSYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8369..9619)
                     /gene="frc"
                     /locus_tag="RH222_003764"
     CDS             complement(8369..9619)
                     /gene="frc"
                     /locus_tag="RH222_003764"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV3798714.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10132..10764)
                     /locus_tag="RH222_003765"
     CDS             complement(10132..10764)
                     /locus_tag="RH222_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV3798715.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3215 a         2465 c         2262 g         3031 t
ORIGIN      
        1 gtcgaattat cttaaaggaa gctcagattt tcttattttt attgagaaaa tgagatgacg
       61 ccttatgtct gtattactac agggagaagg gagatgcttc attgcaaagg gaataatcta
      121 tgaacgcaat aattattgat gaccatcctc ttgctatcgc agcaattcgt aatttattga
      181 tcaaaaacga tattgaaatc ttagcggagt tgactgaagg gggaagcgcc gttcagcggg
      241 tggaaacact taagcctgat atcgtcatca ttgatgtcga tatccccgga gttaacggta
      301 tccaggtgtt agaaacgctg aggaagcgcc aatatagcgg aattattatt attgtctccg
      361 ctaaaaatga ccatttttac gggaaacatt gtgctgatgc tggcgctaat ggattcgtga
      421 gtaaaaaaga aggcatgaac aatatcattg cggctattga agctgcaaaa aatggctact
      481 gctatttccc cttctctctc aaccggtttg ttggaagttt aacgtccgac cagcaaaaac
      541 tcgactcctt atcgaaacaa gaaattagtg tcatgcggta tattcttgat ggcaaggata
      601 ataatgacat tgctgaaaaa atgttcatca gcaacaaaac tgtcagcact tataaaagtc
      661 gcctgatgga aaaattagaa tgtaaatcac tgatggatct ttacacattc gcgcaacgta
      721 acaaaatcgg ctaatcacat gaagttttta ccctattttt ttcttctctg ttgtggtctt
      781 tggtcgacca taagtttcgc agacgaagat tacatcgaat atcgtggcat cagtagtaac
      841 aaccgtgtca cacttgatcc agtacgtctg agcaataagg aattacgttg gttagcgagc
      901 aaaaaaaatc ttgtgattgc agtacacaag tcccaaacgg ctaccttgtt gcataccgat
      961 tcgcagcaac aggttcgtgg tattaatgct gattatttaa atcttttaaa aagagcgtta
     1021 aatatcaaat taacactccg ggaatacgca gatcatcaaa aagcaatgga cgcgcttgca
     1081 gaaggtgaag tcgatatagt gttatcacat ttagttactt cgccgcctct taataatgac
     1141 attgctgcaa ccaaaccatt gataattacc tttccggcgc tggtaaccac ccttcacgac
     1201 tcaatgcgac cgcttacctc accaaaacca gtaaatattg ctcgggtagc aaattacccc
     1261 ccagacgagg taattcatca atcatttcca aaagcaacaa ttatctcttt tacaaattta
     1321 tatcaggcat tagcatccgt ctcagctggg cacaatgatt actttattgg tagtaacatc
     1381 attaccagca gtatgatttc ccgctatttc actcactcct taaatgtagt gaaatattat
     1441 aactcgccgc gtcaatataa ttttttcttg accagaaaag aatctgtcat tcttaatgaa
     1501 gtactcaata gatttgttga tgctttaaca aatgaagttc gctatgaagt atcacaaaat
     1561 tggcttgata caggaaacct ggcctttctg aacaaaccat tagaactcac tgaacatgaa
     1621 aaacagtgga ttaagcagca tcccaattta aaggtgctgg aaaatcctta ctcgcccccc
     1681 tattctatga cggatgaaaa tggctcggtt cggggcgtta tgggggacat tcttaatatt
     1741 attaccttgc aaacaggttt aaatttttct ccgatcaccg tttcacacaa tatccatgct
     1801 ggaacacagc ttagccccgg aggatgggat ataatacctg gcgctattta tagtgaagat
     1861 cgagaaaata atgttttatt tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg
     1921 caaaaagcgc ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc cattccatat
     1981 tattatgagc tgcattcgca attaaaagag atgtatccgg aggttgaatg gatacaggtc
     2041 gataatgcca gcgctgcatt tcacaaggtt aaggaaggtg aacttgatgc tctggtcgcg
     2101 acacagttaa attcgcgtta catgatcgat cattactatc ctaatgaact ttatcatttt
     2161 cttattcctg gcgttccgaa tgcatcgctt tcgttcgctt ttcctcgcgg agaaccggaa
     2221 cttaaggata ttattaataa agcactgaat gcaattcccc caagcgaagt tctgcgcctg
     2281 acggaaaaat ggattaaaat gcccaatgtg accattgaca catgggacct atatagcgag
     2341 caattttata ttgttacgac attatccgtt ttattagttg gcagtagcct tttatgggga
     2401 ttctacctgt tacgctcagt tcgtcgtcgt aaagtcattc agggtgattt agaaaaccaa
     2461 atatcattcc gaaaagcact ctcggattcc ttaccgaatc caacttatgt tgtaaactgg
     2521 caaggtaatg tcattagtca taatagtgct tttgaacatt atttcactgc ggattactac
     2581 aaaaatgcaa tgttaccatt agaaaacagt gactcaccct ttaaagatgt tttttctaat
     2641 gcgcatgaag tcacagcaga aacgaaagaa aatcgaacaa tatacacaca ggtatttgaa
     2701 attgataatg gcatcgagaa aagatgcatt aatcactggc atacattatg caatcttcct
     2761 gcaagtgaca atgcagtata tatttgtggt tggcaagata ttactgaaac gcgtgatcta
     2821 attaatgcac tcgaggtaga aaaaaataaa gcgataaagg ctaccgtagc aaaaagtcag
     2881 tttctggcaa cgatgagtca cgaaataaga acaccaataa gctctattat gggcttcctg
     2941 gaacttctgt cgggttctgg tcttagcaag gagcaacggg tggaggcgat ttcacttgcc
     3001 tacgccaccg gacaatcact cctcggctta attggtgaaa tccttgatgt cgacaaaatt
     3061 gaatcgggta actatcaact tcaaccacaa tgggtcgata tccctacttt agtccagaac
     3121 acttgtcact ctttcggtgc gattgctgca agcaaatcca tcgcattaag ttgcagcagt
     3181 acatttcctg atcattacct ggttaagatc gatcctcagg cgtttaagca ggtcttatca
     3241 aatttactga gcaatgctct caaatttacc accgaggggg cagtaaaaat tacgacctcc
     3301 ctggttcaca ttgatgacaa ccacgctgtt atcaaaatga cgattatgga ttctggaagt
     3361 ggattatcgc aggaagaaca acaacaactc tttaaacgct acagccaaac aagtgcaggt
     3421 cgccagcaaa caggatctgg cttaggctta atgatctgca aagaattaat taaaaatatg
     3481 cagggcgatt tgtcattaga aagtcatcca ggcataggaa caacatttac gatcacaatc
     3541 ccggtagaaa ttagccagca agtggcgact gtcgaggcaa aagcagaaca ccccatcaca
     3601 ctacctgaaa agttgagcat attaatcgcg gatgatcatc cgaccaacag gctattactc
     3661 aaacgccagc taaatctatt aggatatgat gttgatgaag ccactgatgg tgtgcaagcg
     3721 ctacacaaag tcagtatgca acattatgat ctgcttatta ctgacgttaa tatgccgaat
     3781 atggatggtt ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt acccatctgg
     3841 gggcttacag ccaacgcaca ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac
     3901 ttatgtttgt tcaaaccgtt gaccctggat gtactgaaaa cacatttaag tcagttacac
     3961 caggttgcgc atattgcacc tcagtatcgc caccttgata tcgaagccct gaaaaataat
     4021 acggcgaacg atctacaact gatgcaggag attctcatga ctttccagca tgaaacgcat
     4081 aaagatctac ccgctgcgtt tcaagcacta gaagctggcg ataacagaac tttccatcag
     4141 tgtattcatc gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt gattaatatt
     4201 agccatcagt tagaaataac acctgtttca gatgacagta agcctgaaat tcttcagttg
     4261 ctgaactctg taaaagaaca cattgcagag ctggaccagg agattgctgt tttctgtcag
     4321 aaaaatgact aaatagcggc tcccacaatg ttcaaatgtg ggagctattt accagcacat
     4381 cttacattta tgatgagaac tcctggcgaa tttgttcccc atgctggtcg agcgttgccg
     4441 ctcccggcat aacatgcggg tccgcgcagc cgctgatttt tatcggatta cccggcatca
     4501 ttattccccc ggcttcaatc aacatatttc ttgcctgagt ttgtggcaat ttaatggcct
     4561 cagccacact taataacggc gcgacgggta caccaacttc atgtattctg gctaaccaaa
     4621 cttcagctgc ctgcgttttt aacgtccgct caatatattg tttaagaata gcctggtttt
     4681 gtacgcgtaa aatattgctg ctaaatcggg gatcattaac cagttccgta agctccagtg
     4741 cctggcataa cgcagaaaaa agcttgtcat taccacaaca aatcgtaatc ggcttatcct
     4801 gagtattgaa aacatcaaaa ggtgccatgt agggatggcg atttcccaga cgttgtggtg
     4861 acttccctgt cgcgatatat gccatcagac catgctccag aaaactcagc gtggcatcaa
     4921 acatcgctat atcgacatgc gcccctctct ggctcttttc gcggccataa agtgcactca
     4981 ctattccgct gaataaatag acaccgccgc atagatccgc aagagatgta ccaacgcgca
     5041 ctggcggagc atcaggatat cctgtttcca tcattatccc gctcattgcc tgaatgatgg
     5101 tatcgtaggc aggagcatct tttagcggac cggtatgtcc gaaacctgac gatgaagcat
     5161 atatgaggcg cgggttgatt tcttgaagcg tttcccatga aaaccccagt ttttccattg
     5221 tacctgggcg aaaattctca gctaatacat cagcttgttt gagcatattt ataaatatac
     5281 ttttatcgtg atcattcttt aaatcaagaa ccacactctc tttgccatga ttaataaaac
     5341 tgtaatagag tgactgtcca tccacatagg gaccaaatgt gcgggtatca tcaccatgac
     5401 ccggcggctc aactttaatt acccttgcgc ccatattaca aagaagttga gttccgaaag
     5461 gtccattaag gacatgtgtc atatcgataa ctaataagcc ttcaaacggc cctttgcttt
     5521 cattatttgt catttgctta tgccttgaaa aaagccgaag gtgaaaagac acttcggcat
     5581 ttatgtaaaa tcaataacat gattaattat gaaaccagtc gactgacata aatccacaag
     5641 ggagccgtga cgacaaagcc cagtacgctc acagccaatg acgctgtacc agtacgggtg
     5701 tagacattaa accggctggc aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc
     5761 agtaccatca tctgcagatg ttcgctgttc aaatgacatg ccataccaac gagaagcagt
     5821 gccagtggca tcagaatcag cttcaggaag gtgttataag caatttcggc actgaactcg
     5881 aatttatgtg cagccagagt caaccccgca gcgaataccg ctacccctga gttagccttc
     5941 gcaatcagat taaaggttgg gtcccatgct gccggaattt ttaccccaac caacaccagg
     6001 atcgttgcca gaacaggtgc ccatactact ggctcctttg ccgcagaaat taatgcgctc
     6061 agattactat tcttcttacc atccgctcct gaggaaggat tcagcaaata cagaccaata
     6121 ggaatagtaa ttgcgttaac aataatagaa ataattgcta ccactaaacc tgttgatacg
     6181 gaatcaccat aaataggatc gagaactgca aaccccagga atccaatggt aggtgaaccc
     6241 gcaattaatg cacatacagc tgcttctgca tgggtacgtt taaaaaattt gtagcaacca
     6301 aaccaggaga aaaagaaaca tccgacaata accacaagtg atacaagggt cagacgagtg
     6361 tccgcaaaaa tcatttccct gtttgcccgg gtaatagaaa caaatagagc cgcaggaaga
     6421 gcgtagttta ataccagttt attaaatgcg cgggcttgat cttctgaaaa agtttctcgt
     6481 ctgccgctaa aataacccaa cagcatgatt acgataatag gcaataaatc gccaataaaa
     6541 aatgttagca taatagtatt tccgataagg gtcccctcac catcgcataa tgagttggca
     6601 agaggagacg atgtcagtat ttaattacca gcgacttgtt ttgggttaag tttggtaata
     6661 tggccacttt cagttcctgc tgcagggtcg atgaccacat taataatggt cggtttgcgc
     6721 gactgaatac cggtggttaa agcatgacga agttcatctg tcgtggtgac gttatagcca
     6781 acgccacgaa acgcatccat taatttgtca taccttgcat ggtgcaacag atccgttggt
     6841 gatggtgcac cagcgccact gagatcaaca ccgtctcctc tgtagatgcc gccattatta
     6901 aaaataacga tcgtcaccgg caggttatat cgacaaatcg tttcaatttc catcccactg
     6961 aaaccaaaag cactatcacc ttcaatggcg acaaccggag aaccagaagt cacgctagca
     7021 ccgatggcat agcccatacc gatgcccatg acaccccagg taccacaatc cagacgacga
     7081 cgtggtttat acatatcaat aatatttcgt gcattatcca gggtatttgc accttcatta
     7141 actaaataaa tatcctggtt ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag
     7201 taatttaatg gttgagtatc tgtacttaat ttttcatgca ttttttgtgc attttgctgc
     7261 ttgtggatat ttaaaatatc gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt
     7321 tctgccagca taccttgcat actggatgca atatcaccaa cgactggcac agcaatgggg
     7381 cggttgctgt caatttcctg cggttcaata tccagttgaa taaactgtgt atctgccgcc
     7441 catccttttt taccgtgtgc caataaccaa ttcagtcgtg caccaacaag catgacaacg
     7501 tcagcatttg ccagggcaaa cgaacgcgca gctgccgcag aaagtggatg cgtatcttca
     7561 aggatccctt tcgccataga cattggcagg aatggaatct gagtactttc aataaattca
     7621 cgaagctgtt catcagcttg tgaatacgcc gcgcctttgc caaggataat taatggccgt
     7681 tcagcttttg ctaaaagcga aattgcgcta gtgactgact tcgggcatgg caataatgct
     7741 ggcgacggat tttcaacttt aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg
     7801 gccaggacat ttgctggcaa atcaagataa actccgccag ggcgacccga tacagagacc
     7861 cggatagcgc gtgccaatgc aatgccaaga tcctgcggct gattaacgcg aaatgctgct
     7921 ttggcatacg attttgccgc attcatttgg tccagctctt cataatcacc ttgctgtagg
     7981 tcgacgatcg cgcggtcgct ggagccgcta atcatgatca tcggaaaacc atttaccgtt
     8041 gcgttggcca atgcggtcaa accattgagg aatcctggcg cagaaactgt caggcagatc
     8101 cccggttttt gggtaagaaa accgcttgcc gcagcggcat agcctgccga ctgctcatga
     8161 cgaaaaccaa tataacgaat gccttccgcc tgggcatggc gtgccatatc cgtcacagga
     8221 atacctacaa caccataaat agtgtcaata ttattctgtt ttaatgcttc aacgatgata
     8281 tgcataccat ctgtcatttg aagttgatct gacatgatta ataacctcga aatttttata
     8341 aattgggagc cagaagcccc cgttaatatc agatggcgtg gttttgcttc attgcagcaa
     8401 tttcatcgtc actataaccc agctcctgca atacagcagc ggtatgttca cctaatagcg
     8461 gcgcagcttt aatatccggc gtaaaggcag agaatttcat tggacaacca acggtcagat
     8521 attttccacg caacggttgt tccacttcaa caacactgcc actttggcgc aaagagggat
     8581 caagtgaaat ttctttcata cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat
     8641 aggccaccgc ttcatgttta tcaatagtga cagtgtattt ttcgatttca gcaaaaatat
     8701 cgaaaatatg tggctgtcgt gcatgggctg tactgtatgc cggatcggta atccattctg
     8761 gtttgccgat ggctttacag gtgttttccc agttttgctc ctgaatagtg aaataaatat
     8821 aggcgttagg atcggtttcc cagcctttac atttcaggat ccagccaggc tgaccgccac
     8881 cacctgcatt accaccgcgg ggaactgcat caccaaatgt accattcgga tactgcgggt
     8941 attcttccag ataacccaat ttatcgagac gctgctggtc acgtaatttc acgcggcaaa
     9001 ggttcaatac ggcatcctgc attgacatgg tgactcgttg cccacgcccc gttttttcgc
     9061 gatgcagcaa agcagcaagt aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca
     9121 acgctgcagc gcttaccagc ggcggaccat cccaaaaacc cgtagtggat gccgcgccac
     9181 ccgctgcctg agcaacgttt tcataggctt ttacattcac ataaggcgaa cactcatcaa
     9241 accctttgat cgaaccaaaa atcagacgtg gattgatttc ttgaatatgc tcccaggtga
     9301 agcccatgtg atcaatggcc cctggatgaa agttctcgac taagatatca gcttcgcgga
     9361 tcagcttttc cattacctct ttgccttccg ctgttttggt atttaactca atagaacgtt
     9421 tgttactgtt aagcatggtg aagtaaagcg catcgatatc aggaatatct cgcagctggt
     9481 gacgcgttac gtcaccaacg ccgggacgtt caattttaat aacgtcagcg ccaaaccagg
     9541 ccagcatttg agtacaagat gggccagatt gcacaccggt gaaatcgaga actttaattc
     9601 cttgaagtgg agttgacata gcaataccta ttagttaatt aaatatgtgt taatttagcg
     9661 tttatttaag gcgtaatatc ttctataaat aacccgcaaa atatcgtcgt agctgcactc
     9721 gatattttta aaatcctgta tcaagtgctg aaaatcattt catcgccact tccaattcct
     9781 ttctgcatgg taagtagtga ggacgaaaag aagaatatga taaatattga ttcgtggtaa
     9841 tcagagagag tcgcgacatt tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt
     9901 tacaagtaag ggtatgatta cgctttttaa agcgtaccat aaaacataac ataataaatg
     9961 atttgtgatg acacgggatt attgggattt actttgagga agataaaatg cgtgtttata
    10021 gaaccgcgag cgccaaataa ttttatgacc acaacctgtc gtattgtggt cataaaaaat
    10081 tgtcggctgg agggagttaa atcctttacc tcaatatact gcctgtcagc attagttcac
    10141 aaacagtgct tcgctgtcga cgatgattcc atcttccgca cctttaagcg ccaggtttgc
    10201 ctcgtagtat tgctttttat caagcagttt ctgcgcatca gcaagtgcat tacgagtctg
    10261 tttaagcggc atcagatatt ggttttccat tacgccaaca ccggccaggc gaagttcttc
    10321 cattgcgccc tttttatccc ctttcgccat tttttcattg gcaattttta tcgcggcttc
    10381 tttttcggga gttgctacat aactctcaga aattccgaca gatgcattaa tgacaatata
    10441 ttggtcatca ttgagattgg tctttttacc tggcttagcg aactttgccc attcggtact
    10501 gtcatctgac agcaaagcgg aagcttcatt ggtcagttct tttgcctttt ctggatcgcc
    10561 atgaaacaga gccaggcgcg ctacctgcac gtcacgcatc gcatataaac cttgttcaga
    10621 gatacgctct gctgctgccg ttttctgggt ttgctgaact tgctgagttt gctgttgtgc
    10681 cgctaccggt gcattatcag cagcccatac agttgaagat gccagaattg ctgtgaccat
    10741 cgtagccata attaaacgtt tcatacttac ctcattcttt aacagtttta gaaaacgccg
    10801 ccatcatatt gggtgagcat gaaatgagaa atcagagagc aacggtcatt ttgaaagggt
    10861 tatcttacag ttgtagttat tatcgcaaga agtattttag tcttacaatt gtaggaatct
    10921 gcttcctgtc gcagagaact attcttatgt accacaaaga gatgtcattt att
//
LOCUS       DAPHJE010000133        10938 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPHJE010000133 DAPHJE010000000
VERSION     DAPHJE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.134"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            27..650
                     /gene="nfeR"
                     /locus_tag="RH222_003766"
                     /gene_synonym="yqjI"
     CDS             27..650
                     /gene="nfeR"
                     /locus_tag="RH222_003766"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV3798716.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(804..2324)
                     /gene="aer"
                     /locus_tag="RH222_003767"
     CDS             complement(804..2324)
                     /gene="aer"
                     /locus_tag="RH222_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV3798717.1"
                     /translation="MSSHPYVTQQNTPLAGDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVMLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2742..4121
                     /gene="ygjG"
                     /locus_tag="RH222_003768"
     CDS             2742..4121
                     /gene="ygjG"
                     /locus_tag="RH222_003768"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV3798718.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4163..4495)
                     /locus_tag="RH222_003769"
     CDS             complement(4163..4495)
                     /locus_tag="RH222_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV3798719.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4714..5697
                     /gene="ebgR"
                     /locus_tag="RH222_003770"
     CDS             4714..5697
                     /gene="ebgR"
                     /locus_tag="RH222_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV3798720.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQLLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            5881..8973
                     /gene="ebgA"
                     /locus_tag="RH222_003771"
     CDS             5881..8973
                     /gene="ebgA"
                     /locus_tag="RH222_003771"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV3798721.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLSASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGSD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            8970..9419
                     /locus_tag="RH222_003772"
     CDS             8970..9419
                     /locus_tag="RH222_003772"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV3798722.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            9482..10915
                     /locus_tag="RH222_003773"
     CDS             9482..10915
                     /locus_tag="RH222_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3798723.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
BASE COUNT         2616 a         2949 c         2770 g         2603 t
ORIGIN      
        1 atctaattaa gcaaaaggag gctgatatga gccatcatca cgaagggtgt tgtaaacatg
       61 aaggccagcc acgccatgag ggctgctgca aaggtgagaa gtcagaacac gagcactgcg
      121 gacacggtca ccagcatgaa cacggtcaat gctgcggtgg tcgccacggt cgcggcggcg
      181 gtcgtcggca acgtttcttt ggtcacggtg aattgcgtct ggtgattctg gatattctct
      241 cgcgcgatga cagccacggt tacgaattga ttaaagcgat tgagaatcta acccagggga
      301 attacacccc aagcccgggc gtcatctacc cgacgctgga ttttctgcag gagcagtcgc
      361 tgattaccat ccgcgaagag gaaggtggta agaagcagat tgcactgacc gaacaaggcg
      421 cgcagtggct ggaagaaaac cgcgaacagg tggagatgat tgaagaacgc atcaaagcgc
      481 gttgcgttgg cgcggcgctg cgccagaacc cgcaaatgaa gcgggcgctg gataatttta
      541 aagcggtgct ggatttacgc gtcaaccaga gcgatatcac tgatgcacaa ataaaaaaga
      601 tcattgcggt gatcgaccgc gccgcttttg atattacgca actggattaa tcgccgcatc
      661 cgccagtggc gcggtgcaat tgccggatgc gacgcttgac gcgccttatc cggcctacac
      721 ccgctacaca ccccgtaggc ctgataagat gcgccagcat cgcatcaggc attgtgctcc
      781 aacagccgga tccggcgtac cgattaatgc agcaccgtca ccgcgtcttc cagtcggctg
      841 gcgcggtgtt tcaccatcgc cgacacctgc gcactctctt ccaccagctc ggcatttttc
      901 tgggtgatca ggttaagctc atccactgca cgggtcaggc tggaaagccc atcggcctgt
      961 tccagcgttg aatggctaat ctgggcgatc aactgggtga cgtttttcac ctgtgccaca
     1021 atatcttcca tcgtccgtcc ggcggcatgt acctgctgcg aaccggattg caccttatca
     1081 gcactggcat caatcagctt gcgaatatcg ttggcagcat tagcgctgcg gctggctaaa
     1141 tgacgcactt cccccgccac cactgcaaaa cctttgccct gttcgccggc acgcgccgct
     1201 tccaccgccg cattcagggc cagaatattg gtctgaaacg caatatcgtt aatcagcgaa
     1261 gtaatggtgc caatgcgctg ggtactgtcg gcaatatcgt ccattgtctt gatcaccgta
     1321 gtcatcgcct ccccgccctg cactgccgca ttactggcgg tgatagaaag tttatcggca
     1381 gccgacgccg tggcagagtt ctgtttcacc gacgccgcca tttggttcat ggtcgccacc
     1441 gtttgctgaa cgttatcaac tgtctgctgg gtatgttcgt tcagttcatc ggtgcctttc
     1501 gccagcgtct cactgccatt tctgacactg gacacctggc ttgagacatc gttaattagc
     1561 caacggcaca tcaggccaag ctgcccaacc gcgcgtaatg tcagccccag ctcatcgctg
     1621 cgattcagat gctcaacact attacgctct ccggtcgcca ccttcagtgc ctgacgggcg
     1681 acattttcta tcgggcggac aatctgccat tcaaaacagg cgcttgccaa caacatcact
     1741 aacgcacaga ggatatacgt caccaccggg gcagcaacaa accaaagcat ggccgccagc
     1801 aagataaaca tcagggtcat cactccacgc gcccgccagc gaagcggtaa tgaaggcagt
     1861 ttacccagcc agcctttacg caccaccagg cctttatgaa tacgcttacc ggtacgtccg
     1921 gcgttcagcg ctttgtacag cggctccacc gccgcgatct cttcatccgt cgcccgggta
     1981 cgaatcgaca tatagccact gatttttccc tcgcgcacca tcggtaccgc attggcccgc
     2041 acccaataat ggtcaccatt tttgcggcga tttttcacga tgccgctcca gggctcccct
     2101 tttttcaggg tgaaccacat atccgcaaac gccgcttttg gcatatccgg gtgacgcacc
     2161 atgttgtgcg gctgcccttg taactcttgc aaggtaaagc cgctcacctg cacaaaagtg
     2221 tcattagcat gagtgatata gctttgcaga tcggtagtgg acatcagagt ggtatcgccc
     2281 gccagcgggg tattttgctg ggtgacatac ggatgagaag acatgttcgc gtcctgtgca
     2341 ggttatatgg ttgttaactt cttgtcagag tttatgtcgg ccccgctgcg gttatcttta
     2401 accgattaat ttgatttaga tcgcaatttg cgattaaagc acaaatctaa ttctttgatt
     2461 taaaatactt tcactctgtt actatacgaa aacgttaatt atcttgccca aaaatcaggc
     2521 aattattgcc ctgaaaacgt gcatttgcgc agcaatcatc aaatccatac ccgacaaaaa
     2581 ccgtgcgaaa taacaacaga tgttaacaaa tagcattaaa tattgcgcaa atgataacca
     2641 catttgtgtt tatcccgatt ttcgcgatcg cagccggagt ggcgcaatcc ctgcaatact
     2701 taaatcggta tcatgtgata cgcgagcctc cggagcatat tttgaacagg ttaccttcga
     2761 gcgcatcggc tttagcgtgc agcgcccacg ccctgaatct cattgagaag cgaacgctgg
     2821 atcatgagga gatgaaagca cttaaccgag aggtgattga atacttcaaa gagcatgtca
     2881 atccggggtt tttagagtat cgcaaatctg ttaccgccgg cggggattac ggagccgtag
     2941 agtggcaagc gggaggttta aatacgcttg tcgacaccca gggacaggag tttatcgact
     3001 gcctgggagg ttttggaatt ttcaacgtgg ggcaccgtaa tccagttgtg gtttccgccg
     3061 tacagaatca acttgcgaaa caaccgctgc acagccagga gctgctcgat ccgttacggg
     3121 cgatgttggc gaaaaccctt gctgcgctaa cgcccggtaa actgaaatac agcttcttct
     3181 gtaatagcgg caccgagtcc gttgaagcag cgctgaagct ggcgaaagct taccagtcac
     3241 cgcgcggcaa gtttactttt attgccacca gcggcgcgtt ccacggtaaa tcacttggcg
     3301 cgctgtcggc cacggcgaaa tcgaccttcc gcaaaccgtt tatgccgtta ctgccgggct
     3361 tccgtcatgt gccgtttggc aatatcgaag ccatgcgcac ggctcttaac gagtgcaaaa
     3421 aaaccggtga tgatgtggct gcggtgatcc tcgaaccgat tcagggtgaa ggtggcgtaa
     3481 ttctgccgcc gccgggctat ctcaccgccg tacgtaagct atgcgatgag ttcggcgcac
     3541 tgatgatcct cgatgaagta caaacgggca tggggcgcac gggcaagatg ttcgcctgcg
     3601 agcatgagaa cgtacagccg gatatcctct gccttgccaa agcgctcggc ggcggcgtga
     3661 tgccgattgg cgcgaccatc gccactgaag aggtgttttc agttctgttc gacaacccat
     3721 tcctgcatac caccaccttt ggcggcaacc cgctggcctg tgcggcggcg ctggcgacca
     3781 tcaatgtgtt gctggagcag aacttaccgg ctcaggctga gcaaaaaggc gatatgttgc
     3841 tggacggttt ccgtcaactg gcgcgggaat atcccgatct ggtacaggaa gcgcgtggta
     3901 aagggatgtt gatggcaatt gagtttgttg ataacgaaat cggctataac tttgccagcg
     3961 agatgttccg ccagcgcgta ctggtggccg gaacgctcaa taacgccaaa acgatccgca
     4021 ttgaaccgcc actgacactg accattgaac agtgtgaact ggtgatcaaa gcggcgcgta
     4081 aggcgctggc ggccatgcga gtaagtgtcg aagaagcgta atatcaaatc ggatggcgat
     4141 acgacgtcgc catccgatct ttttatacga cgcgtacgcc cgcaggcatc atccgctccg
     4201 gcgttaacaa cacgctttca ctgccgtcat cggtttccgc acacaacagc atgcattccg
     4261 acgtttcacc acgcattttc gctttttgca gattgcataa gaccaccacc gttttcccca
     4321 tcagctcttc ttcgctgtag tacggcacca ggctggtcac ggtttgcagt gttttttccc
     4381 ccacatcaac ctgtacgatg tacagcttgt cggcgttttc atggcgtttc acttccacaa
     4441 tctttccgac gcgcatttcc agacgtgcaa aatcagcgta agccacggtt tccattgctt
     4501 cctcccttca agtaaaattt tactaaacta tagcaaaagt ttttctcaat cctgtaggct
     4561 aaaaatggag aatgcaggcg tgatcacatt cgtgagccgc tgtgttaccg ttacagcgtc
     4621 aaagaaacgc gctttattta ctgaaaacag gtgacccgat aagcacttcc tctacaatgg
     4681 gggcgcacat cagggaaagt aaaaaaggta aacatggcaa cactaaaaga catcgcaatc
     4741 gaagctggcg tatccctggc gacagtatcc agggtcttaa atgacgatcc gacattgaat
     4801 gtgaaagaag agacgaaaca tcgcattctc gagatcgccg aaaagctgga gtacaagacc
     4861 agtagtgccc gtaaactcca gacaggcgca gtcaaccaac accatattct ggctatctac
     4921 agctaccaac aggagctgga gatcaacgat ccttactatc tggcgatccg ccacggcatt
     4981 gaaactcagt gcgaaaagct gggcattgag ctcaccaact gttatgaaca cagcggatta
     5041 ccagacatta aaaacgtcac cggtatttta attgtcggca aacccacgcc cgccctgcgc
     5101 gccgccgcct gtgcgttgac cgacaatatc tgttttatcg actttcacga acccggcagc
     5161 ggttacgatg cggtggatat cgatctggca cgcatcagta aagaaatcat cgacttctat
     5221 atcaatcagg gcgttaatcg tattggtttt attggcggtg aagatgagcc aggcaaagcg
     5281 gatattcgtg aggtcgcctt tgcggaatat ggccgactga aacaagtggt acgcgaagag
     5341 gatatctggc gcggaggctt ttccagttcg tcgggctatg aactggcaaa acagctgctg
     5401 gcgcaggaag actatccgaa ggcactgttt gttgcttctg attccattgc tatcggcgta
     5461 ctgcgggcaa ttcatgaacg tggcctgaac atcccacagg atatttcgct tatcagcgtt
     5521 aacgatatcc ccaccgcgcg atttaccttt ccgccgctct ccaccgtgcg catccattcc
     5581 gagatgatgg gaagtcaggg cgttaacctg gtgtatgaaa aagcccgcga tggtcgcgcg
     5641 ctgccgctgt tggtcttcgt tcccagcaaa ttaaaactgc gcggcacgac ccgttaaatc
     5701 cccttgcacg ctgtccggca atcgtttttg ccggacagtg ctgccgttta ttttcgtgat
     5761 ccagttaaag taaatgcatt tacccgctac tttttagtaa aaattttact aaactcccca
     5821 gcaattacac aaactaccat caccatgaat ggttccgatt tctctctacc gggaggccct
     5881 atgaatcgct gggaaaacat tcagctcacc cacgaaaacc gacttgcgcc acgtgcgtac
     5941 tttttttcat atgattctgt tgcgcaagcg cgtacctttg cccgcgaaac cagcagcctg
     6001 tttctgccct taagcggcca gtggaatttc cacttttttg accatccgct gcaagtgcct
     6061 gaagccttca cctctgagtt aatggctgac tgggggcata ttaccgtccc cgccatgtgg
     6121 caaatggaag gtcacggcaa actgcaatat accgacgaag gttttccgtt ccccatcgat
     6181 gtgccgtttg tccccagcga taacccaacc ggtgcctatc aacgtatttt caccctcagc
     6241 gacggctggc agggtaaaca gacgctgatt aaatttgacg gcgtcgaaac ctattttgaa
     6301 gtctacgtta acggtcagta tgtgggtttc agcaagggca gtcgcctgac cgcagagttt
     6361 gacatcagcg cgatggttaa aaccggcgac aacctgttgt gtgtgcgcgt gatgcaatgg
     6421 gcggactcta cctacgtgga agaccaggat atgtggtggt cagcggggat cttccgcgat
     6481 gtttatctga tcggaaaaca actaacacat attaacgatt tcaccgttcg taccgacttt
     6541 gacgaagcct attgcgatgc cacgctttcc tgcgaagtgg tgctggaaaa tctctccgcc
     6601 tcccctgtcg tcacgacgct ggaatatacc ctgtttgatg gcgaacgcgt ggtgcacagc
     6661 agcgccattg atcatttggc aattgaaaaa ctgaccagcg ccagctttgc ttttactgtc
     6721 gaacagccgc agcaatggtc agcagaatcc ccttatcttt accatctggt catgacgctg
     6781 aaagacgcca acggcaacgt tctggaagtg gtaccacaac gcgttggctt ccgtgatatc
     6841 aaagtgcgcg acggtctgtt ctggatcaat aaccgttatg tgatgctgca cggcgtcaac
     6901 cgtcacgaca acgatcatcg caaaggccgc gccgttggaa tggatcgcgt cgagaaagat
     6961 ctccagttga tgaagcagca caacatcaac tccgtgcgta ccgctcacta cccgaacgat
     7021 ccgcgttttt acgaactgtg tgatatctac ggcttgtttg tgatggcgga aaccgacgtc
     7081 gaatcgcacg gctttgctaa tgtcggcgat atcagccgta ttaccgacga tccgcagtgg
     7141 gaaaaagtct acgtcgagcg cattgttcgc catattcacg cgcagaaaaa ccatccgtcg
     7201 atcatcatct ggtcgctggg caatgaatcc ggctatggct gtaacatccg cgcgatgtac
     7261 cacgcagcga aggcgctgga tgacacgcga ctggtgcatt acgaagaaga tcgcgatgct
     7321 gaagtggtcg atattatttc caccatgtac acccgcgtgc cgctgatgaa tgagtttggt
     7381 gaatacccgc atccgaagcc gcgcatcatc tgtgaatatg ctcatgcgat ggggaacgga
     7441 ccgggcgggc tgacggagta ccagaacgtc ttctataagc acgattgtat tcagggacat
     7501 tatgtttggg agtggtgcga ccacgggatc caggcgcagg atgacaacgg caatgtctgg
     7561 tataaattcg gcagcgacta cggcgactat cccaacaact ataacttctg tcttgatggt
     7621 ttgatctatt ccgatcagac gccgggacca ggcctgaaag agtacaaaca ggttatcgcg
     7681 ccggtaaaaa tccacgcgct ggatctgact cgcggcgagc tgaaagtcga aaataaactg
     7741 tggtttacca cgcttgatga ctacaccctg cacgcagagg tgcgcgccga aggtgaaacg
     7801 ctcgcgacgc agcagattaa actgcgcgac gttgcgccga acagcgaagc ccccttgcag
     7861 atcacgctgc cgcagctgga cgcccgcgaa gcgttcctca acattacggt gaccaaagat
     7921 tcccgcaccc gctacagcga agccgggcat tctatcgcca cttatcagtt cccgctgaag
     7981 gaaaacaccg cgcaaccagt gcctttcgca ccaaataatg cgcgtccgct gacgctggaa
     8041 gacgatcgtt tgagctgcac cgttcgcggc tacaacttcg cgatcacctt ctcaaaaatg
     8101 agtggcaaac cgacatcctg gcaggtgaat ggcgagtcgc tgctgacccg cgagccaaag
     8161 atcaacttct tcaagccaat gatcgacaac cacaagcagg agtacgaagg gctgtggcaa
     8221 ccgaatcatt tgcagatcat gcaggaacat ctgcgcgact ttgccgtaga acagagcgat
     8281 ggtgaagtgt tgatcatcag ccgcacagtt attgccccgc cggtgtttga cttcgggatg
     8341 cgctgcacct acatctggcg catcgctgcc gatggccagg ttaacgtggc gctttccggc
     8401 gagcgttacg gcgactatcc gcacatcatt ccgtgcatcg gtttcaccat ggggattaac
     8461 ggcgaatacg atcaggtggc gtattacggt cgtggaccgg gcgaaaacta cgccgacagc
     8521 cagcaggcta acatcatcga tatctggcgc agcaccgtcg atgccatgtt cgagaactat
     8581 cccttcccgc agaacaacgg caaccgtcag catgtccgct ggacggcact gactaaccgc
     8641 cacggcaacg gtttgctggt ggttccgcag cgcccaatta acttcagcgc ctggcactat
     8701 acccaggaaa acatccacgc tgcccagcac tgtaacgagc tgcagcgcag tgatgacatc
     8761 accctgaacc tcgatcacca gctgcttggc ctcggctcca actcctgggg cagcgaggtg
     8821 ctggactcct ggcgcgtctg gttccgtgac ttcagctacg gctttacgtt gctgccggtt
     8881 tctggcggag aagctaccgc gcaaagcctg gcgtcgtatg agttcggcgc agggttcttt
     8941 tccacgaatt tgcacagcga gaataagcaa tgaggatcat cgataactta gaacagttcc
     9001 gccagattta cgcctctggc aagaagtggc aacgctgcgt tgaagcgatt gaaaatatcg
     9061 acaacattca gcctggcgtc gcccactcca tcggtgactc attgacttac cgcgtggaga
     9121 cagactccgc gaccgatgcg ctatttaccg ggcatcgacg ctattttgaa gtgcattact
     9181 acctgcaagg gcagcaaaaa attgaatatg cgccgaaaga gacattacag gtagtggaat
     9241 attatcgtga tgaaactgac cgtgaatatt taaaaggctg cggagaaacc gttgaggtcc
     9301 acgaagggca aatcgttatt tgcgatatcc atgaagcgta tcggtttatc tgcaataacg
     9361 cggtcaaaaa agtggttctc aaagtcacca tcgaagatgg ttatttccat aacaaataac
     9421 aactacggcg gcaaaaggag tttgccgcca ccgctaccct acacattttc ggagatgtgt
     9481 tatgtctgat accaaacgta atacaatcgg caaattcggc ttgctctcgc tgacttttgc
     9541 cgccgtttac agctttaaca acgttatcaa taataatatt gagcttggac tggcctcggc
     9601 accgatgttt ttcctcgcga cgatttttta ttttattccc ttctgtctga tcatcgcaga
     9661 atttgtttcg ttaaataaaa actcagaagc cggtgtctac gcgtgggtaa aaagttcgct
     9721 gggcggacgt tgggcattta ttactgccta tacctactgg ttcgtaaacc tgttcttttt
     9781 cacctcactg ttgccgcgcg ttattgctta tgcttcgtat gccttcctcg gctacgaata
     9841 tattatgacg ccggttgcca ccaccattat cagtatggtg ctgttcgcct tctccacctg
     9901 ggtttccacc aacggggcga aaatgttggg gccaattacc tccgtcactt caacgctgat
     9961 gctgctgtta acgctctcct acattttact ggcaggtacg gcgctggttg gcggcgtaca
    10021 gcctgctgac cccatcaccg ttgacgcgat gatcccgaac ttcaactggg cgttcctcgg
    10081 cgttaccacc tggatcttta tggccgcagg tggcgcggag tccgtcgctg tgtacgttaa
    10141 cgacgtcaaa ggcggttcga aatcgttcgt taaagtgatc atcctcgccg ggatttttat
    10201 cggcgtactg tattccgtct cctcggtgct gattaacgtc ttcgtcagca gcaaagagtt
    10261 gaaatttacc ggcggatcgg tgcaggtatt ccacggcatg gcggcgtatt ttggtctacc
    10321 ggaagcgttg atgaatcgct ttgtcggtct ggtgtccttt accgcgatgt tcggttccct
    10381 gctgatgtgg accgcaacgc cggtgaaaat tttcttctcc gaaatcccgg aaggcatctt
    10441 tggtaagaaa accgtcgaac tgaacgaaaa cggcgttccg gcgcgcgcag cgtggatcca
    10501 gttcctgatc gtcatcccgc tgatgattat cccgatgctc ggttccaata ccgtgcagga
    10561 tctgatgaat actattatta atatgactgc cgcagcgtcc atgcttccgc cgttattcat
    10621 catgctggct tacctgaatt tacgcgccaa attagatcac ctgccacgcg atttccgtat
    10681 gggctcccgc cgcaccggta ttatcgttgt ttcaatgctg attgcgatat ttgccgtagg
    10741 gtttgtcgct tcgacattcc cgactggcgc gaatattctg accatcattt tttataacgt
    10801 cggcggtatt gttatcttcc ttggctttgc gtggtggaaa tacagtaaat atataaaggg
    10861 attaacggct gaagagcgcc atattgaagc gacgccagcc agcaatgttg attaagcaga
    10921 ataataaaaa atgagcag
//
LOCUS       DAPHJE010000134        10907 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPHJE010000134 DAPHJE010000000
VERSION     DAPHJE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.135"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(83..167)
                     /locus_tag="RH222_003774"
     tRNA            complement(83..167)
                     /locus_tag="RH222_003774"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Tyr,seq:gta)
     gene            complement(327..1169)
                     /gene="purU"
                     /locus_tag="RH222_003775"
     CDS             complement(327..1169)
                     /gene="purU"
                     /locus_tag="RH222_003775"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV3798724.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1219..1677)
                     /locus_tag="RH222_003776"
     CDS             complement(1219..1677)
                     /locus_tag="RH222_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV3798725.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1790..2695
                     /gene="rssA"
                     /locus_tag="RH222_003777"
     CDS             1790..2695
                     /gene="rssA"
                     /locus_tag="RH222_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV3798726.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRQAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2787..3800
                     /gene="rssB"
                     /locus_tag="RH222_003778"
     CDS             2787..3800
                     /gene="rssB"
                     /locus_tag="RH222_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV3798727.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            4002..4910
                     /gene="galU"
                     /locus_tag="RH222_003779"
     CDS             4002..4910
                     /gene="galU"
                     /locus_tag="RH222_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV3798728.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(5054..5467)
                     /gene="hns"
                     /locus_tag="RH222_003780"
     CDS             complement(5054..5467)
                     /gene="hns"
                     /locus_tag="RH222_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV3798729.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            6071..6688
                     /gene="tdk"
                     /locus_tag="RH222_003781"
     CDS             6071..6688
                     /gene="tdk"
                     /locus_tag="RH222_003781"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV3798730.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFAEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(6990..9665)
                     /gene="adhE"
                     /locus_tag="RH222_003782"
                     /gene_synonym="adhC"
     CDS             complement(6990..9665)
                     /gene="adhE"
                     /locus_tag="RH222_003782"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV3798731.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            10142..10789
                     /gene="ychE"
                     /locus_tag="RH222_003783"
     CDS             10142..10789
                     /gene="ychE"
                     /locus_tag="RH222_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV3798732.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         2799 a         2447 c         2686 g         2975 t
ORIGIN      
        1 gtcgaagctt ctcatccttc cccgcatggg cagaatattt aattacggat tcgtttgaga
       61 gttcagggac ttttgaaagt gatggtggtg ggggaaggat tcgaaccttc gaagtcgatg
      121 acggcagatt tacagtctgc tccctttggc cgctcgggaa ccccaccacg gggtaatgct
      181 ttttactggc ctgctccctt atcgggaagc ggggcgcatc atatcaaatg acgcgccgct
      241 gtaaagtgtt acgttgagaa aaatgaactg gttgcgtaat tttcatccgt aacggattaa
      301 aggtaaccag ttatttttgc tggcgattaa agaataatcg tccgattacc gtaaacaaag
      361 acgcgctgtg ccagtacttt gtatagcgca cgacttaaga cgtttttctc gacgtcacga
      421 cctgcgcgca tcatatcttc agctgtgtag gtatgatcga catgaataac gtcctgcatg
      481 atgattgggc cttcgtccag attgtcattc acatagtgag cggttgcgcc aataatcttc
      541 acaccacgtt cataggcctg gtgataagga cgtgcgccga taaacgcagg caggaatgaa
      601 tggtgaatat tgatgatttt attcgggaag cgtgccacaa attccggcgt taatacccgc
      661 atatacttcg ccagcaccac atagtcaggt tgataagcat caatggcgtc cgccatcttt
      721 tgatcgtgct cgttgcggct taacccttca tggcttacca gctcaaacgg aatatcaaaa
      781 cgctcaacca gagaacgtaa agtatcgtgg ttaccaataa ctgccgcgat ttcgacatcc
      841 aggccgccgt aattggcttt catcaacaaa tcgccaaggc aatgcgcttc tttagtgacc
      901 agaatcacta tccggcgacg accggcagga ttcagctcac gcacggagcc ttctggcaat
      961 gcgctatcga gatccgccag aagggtggaa tcattaaaaa tcccttccag ttccgtgcgc
     1021 ataaaaaagc gcccggtacg gtgatcaaca aattcattgt tctgtacgat atttaactcg
     1081 tgcttgtagc aaatattggt aatacgtgcg atcagacctt tttggtccgg acaaatagta
     1141 cgcagaactt tacgttggag tgaatgcatt gctggaaaaa ccttgttgag agtgtttgct
     1201 aaaccgtacc gtcaaccatt attggccgca gcacttttta aattttttac ctgaaccaca
     1261 agggcaggga tcgttgcgac caaactgcgg acgtgtaccg tcaatatagt accattgacc
     1321 gttttctttc aaaaatcgcg aacgttcaat tattgcacct gttttgccac cttcggtaaa
     1381 tcgagcaaca aaactgacaa atccgatatt gtcggcgtcc tgccaacaat gttcaaatac
     1441 ggtcaatccc aaccattcag tatgcgcaaa tccggccatc aattcggcac gtaacgccgc
     1501 tgctccacaa gagggatgcc aggtctttat taaataatct gcgtcttgca tcacaaaagc
     1561 gcagtaacgc gaacgcatga gatgttctgg atcaggtgca accttttcac cagacacata
     1621 agggtggcaa cataggctat actcgacagc actaccacag ggacaaagct gagacacaaa
     1681 taatctccct ggaaacaata acggcgtatt aaccgcctga gtagcactat gttaaccgag
     1741 cagtagcgat gtggctacga ttgcattcca ggggaatctt gcgggaataa tgagaaagat
     1801 aaaaataggg ctggcgctgg gatctggcgc ggcgagaggt tggtcgcata ttggcgttat
     1861 taatgcgcta aaaaaagtgg gtattgaaat tgatatcgtt gcaggatgtt caattggttc
     1921 gctggtgggc gcggcctatg catgcgatcg attatctgcg ctggaagatt gggtaacctc
     1981 tttcagttat tgggacgttt tacgcctgat ggatctctcc tggcagcgcg gtgggttact
     2041 gcgcggcgag cgtgtcttca atcaataccg cgaaataatg ccggaaacag agatcgaaaa
     2101 ttgttcccgt cgctttgcgg ctgttgccac caatttaagt acgggacgtg aattatggtt
     2161 tactgaaggc gatctccatc ttgctattcg cgcatcatgc agtattccag gactcatggc
     2221 acctgttgca cataacggct actggctggt tgatggagca gtcgttaacc caattcctat
     2281 ttcactcacg cgtgcattgg gtgctgatat tgtgatagcg gtcgacctgc agcacgatgc
     2341 tcatttgatg caacaagatt tgctctcctt taatgtcagt gaagaaaata gcgagaatgg
     2401 tgattctctg ccgtggcatg cgcgtctgaa agaaagatta ggcagcataa cgacacgtca
     2461 ggcggtgaca gcaccaacgg caacagagat tatgaccact tctatccagg tgctggagaa
     2521 ccgccttaaa aggaaccgca tggcaggtga tccgcccgat attctgattc aacctgtttg
     2581 cccgcaaata tctacgcttg atttccatcg cgcgcacgct gccattgcgg ccggacagct
     2641 ggcagtggaa aagaaaatgg acgaactttt gccgttggta cgcaccaaca tttgaccaga
     2701 atttttatct acacttaagt taattctgac aggcgcaggt ggcaatagca tgccactatt
     2761 gagtaaagcc agtcagggga gagaacatga cgcagccatt ggtcggaaaa cagattctca
     2821 ttgttgaaga tgagcaggta tttcgctcgc ttctggattc gtggttttcc tcattgggag
     2881 cgacaacggt actggcggct gatggggtgg atgcccttga gttgctggga ggtttcactc
     2941 cagacctgat gatatgtgat atcgcgatgc cacgaatgaa cgggcttaaa ctgctggagc
     3001 atatacgtaa cagaggcgac cagaccccag ttctggtgat atctgccact gaaaatatgg
     3061 cagatattgc caaagcgtta cgtctgggcg ttgaagatgt tttgctgaaa ccagttaaag
     3121 atctgaatcg cttgcgcgag atggtttttg cctgtctcta tcccagcatg tttaattcgc
     3181 gcgttgagga agaggaaagg ctttttcgcg actgggatgc aatggttgat aaccctgccg
     3241 cagcggcgaa attattacag gaactacaac cgccggttca gcaggtgatt tcccattgcc
     3301 gggttaatta tcgtcaattg gttgccgcgg acaaacccgg cctggtgctt gatattgccg
     3361 cactttcgga aaacgatctg gcattttatt gccttgatgt aacccgagct ggacataatg
     3421 gcgtacttgc tgccttgtta ttacgcgcat tgtttaacgg attattacag gaacagcttg
     3481 cacaccaaaa tcaacggttg ccagagttgg gcgcgttatt gaagcaggta aaccatttac
     3541 tccgtcaggc caatctgccg gggcagtttc cgctattagt tggctattat catcgcgaac
     3601 tgaaaaatct cattctggtt tctgcgggtc tgaatgcgac gttaaatacc ggcgaacacc
     3661 aggtgcaaat cagtaatggt gttccgttag gcactttagg taacgcttat ttgaatcaat
     3721 tgagccagcg atgcgatgcc tggcaatgcc aaatatgggg aaccggtggt cgactgcgct
     3781 tgatgttgtc tgcagaatga gcaaacgata acgcgggcta aatttgcatt acctgctaat
     3841 gtcggctggt ggtactatcg tcgccattcg tataagtaat tgtcttaatt atgctaactc
     3901 gcctcctttt cagaacttag ccccttcggg gtgctgatat actgggatgc gatacagaaa
     3961 tatgaacacg ttcaaaacac gaacagtcca ggagaattta aatggctgcc attaatacga
     4021 aagtcaaaaa agccgttatc cccgttgcgg gattaggaac caggatgttg ccggcgacga
     4081 aagccatccc gaaagagatg ctgccacttg tcgataagcc attaattcaa tacgtcgtga
     4141 atgaatgtat tgcggctggc attactgaaa ttgtgctggt tacacactca tctaaaaact
     4201 ctattgaaaa ccactttgat accagttttg aactggaagc aatgctggaa aaacgtgtaa
     4261 aacgtcaact gcttgatgaa gtgcagtcta tttgtccgcc gcacgtgact attatgcaag
     4321 ttcgtcaggg gctggcgaaa ggcctgggac acgcggtatt gtgtgctcac ccggtagtgg
     4381 gtgatgaacc tgtggctgtt attttgcctg acgttattct ggatgaatat gaatccgatt
     4441 tgtcacagga taacctggca gagatgatcc gccgctttga tgaaacgggt catagccaga
     4501 tcatggttga accggttgct gatgtgaccg catatggcgt tgtggattgc aaaggcgttg
     4561 aattagcgcc gggtgaaagc gtaccgatgg ttggcgtggt tgaaaaacca aaagcggatg
     4621 ttgcgccgtc taatctcgct attgtgggtc gttacgtact tagcgcggat atttggccgt
     4681 tgctggcaaa aacccctccc ggagctggtg atgaaattca gctcaccgac gcaattgata
     4741 tgctgatcga aaaagaaacg gtggaagcct atcatatgaa agggaagagc catgactgcg
     4801 gtaataaatt aggttacatg caggccttcg ttgaatacgg tattcgtcat aacacccttg
     4861 gcacggaatt taaagcctgg cttgaagaag agatgggcat taagaagtaa catccgtatc
     4921 ggtgttatcc acgaaacggc gttgagcaat cgacgccgtt tttttatagc ttattcttat
     4981 taaattgtct taaatcggac aataaaaaat cccgccgatg gcgggatttt aagcaagtac
     5041 aatctacaaa agattattgc ttgatcagga aatcgtcgag ggatttacct tgctcatcca
     5101 ttgctttttt gattacagct ggagtacggc cctggccagt ccaggtttta gtttcgccgt
     5161 tttcgtcaac gtagctatat tttgccggac gctgagcacg tttagctttg gtgccagatt
     5221 taacggcagc aaggctattc agcagttcgt tcgggtcaat accgtcagcg atcagcattt
     5281 cgcgatattg ctgcagttta cgagtgcgct cttcaacttc agcagcagcc gcgctttctt
     5341 cttcgcgacg ttcgttaaca acaacttcta atttttccag catttcttcc agcgtttcaa
     5401 gtgtacattc tcttgcctgc gcacgaagag tacggatgtt gttcagaatt ttaagtgctt
     5461 cgctcattgt agtaatctca aacttatatt ggggtggttt gttgaggtaa taatagagcc
     5521 ttaaattcag ttgtgcaata gccaggaatg taaggaattc aaaattgttc tttattttgt
     5581 gccgccaata aatatctttt cataaaatta gccagaaaag acgcggcata tagccctatt
     5641 tgcaccgatg atttcgcagc tcgtgaggtt aaaacttcct gattcatgtc acattttatg
     5701 gggagattat cgtaggctga cgacctttca gtcttctgta ttagtcgtgt ttacgagaat
     5761 tccctattaa gcgaatgatg aaaagtagaa cagtcgcaat aagagcaggg acttagtatt
     5821 gcactatctc ctggaggtca acagagggct attacttgcg caacaggtta aagattgtga
     5881 atagttacca gcagtcattt acccgcttat aacaagcgag gcaatagtaa tgatagctca
     5941 gaaggattat gcaaggcttc gtaagggaga acgcatatac cacttctgtg catactgttg
     6001 agctgaaaaa ctgacgaatt atgataaact ccagccaacg ttatttcata tcattgaggg
     6061 cctgtggctg atggcacagc tatatttcta ctattccgca atgaatgcgg gtaagtctac
     6121 agcattgttg caatcttcat acaattacca ggaacgcggc atgcgcactg tcgtatatac
     6181 ggcagaaatt gatgatcgct ttggtgccgg gaaagtcagc tcgcgtatag gtttgtcatc
     6241 gcctgcaaaa ttatttaacc aaaattcatc attatttgct gagattcgtg cggaacatga
     6301 acagcaggca attcattgcg tactggttga tgaatgccag tttttaacca gacaacaagt
     6361 atatgaatta tcggaggttg tagatcaact cgatataccc gtactttgtt atggtttacg
     6421 taccgatttt cgaggtgaat tatttattgg tagccaatac ttactggcat ggtccgacaa
     6481 actggttgaa ttaaaaacca tctgtttttg tggccgtaaa gcaagcatgg tgctgcgtct
     6541 tgatcaagca ggcagacctt ataacgaagg tgagcaggtg gttattggtg gtaatgaacg
     6601 ctacgtttct gtatgccgta aacactataa agaggcgtta caagtcggct cattaacggc
     6661 tattcaggaa aggcatcgcc acgattaata agaatttctt tactgacagg gtgagcaggg
     6721 cacttttatc ctgtcagttc gttttacgca cttcttccgg gctatacacc cttctcggca
     6781 gttttttaac gccgctatac gcctcacagg gctcttaagc accgacgttg acttgtgacc
     6841 tgtaaagtac aatatccctg tgtttacgcg ttatacatcg tcgcaaatat gatgaaggct
     6901 aatgctgtcg gtttatggaa aagttgcttt gggtaaacaa aaaaacggcc ccagaagggg
     6961 ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt tttctcagct
     7021 ttagccggag cggcttcttt tttcgctgca gtttcacctt ctacataatc acgaccgtag
     7081 taggtatcca gcagaatctg tttcagctcg gagatcagcg ggtaacgcgg gttagcgccg
     7141 gtgcactggt catcgaatgc atcttcagac agtttatcca cgttcgccag gaagtctgct
     7201 tcctgaacgc cagcttcacg gatagatttc ggaataccca gttcagcctt cagcgtttcc
     7261 agccatgcca gcagtttctc gatcttagca gcagtacggt cgcccggtgc gctcagaccc
     7321 aggtggtcgg caatttcagc ataacgacgg cgagcctgcg gacggtcata ctggctgaat
     7381 gcagtctgct tggtcgggtt gtcgttcgca ttgtagcgaa taacgttaca aatcagcagg
     7441 gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca gtttgtgcgc cattgagtga
     7501 catacaccca ggaaggcgtt cgcaaacgcg atacccgcga tagtcgctgc actgtgaaca
     7561 cgttcacgcg ctaccggatt tttagaccct tcgtggtagg acgctggcag atattctttc
     7621 agcagtttca gtgcctgcag agcctgacca tcagagaact cagatgccag tacagaaaca
     7681 taagcttcca tggcgtgagt tactgcgtcc agaccaccga aagcacacag ggacttcggc
     7741 atgtccataa ccaggttggc gtcgacaatc gccatatccg gggtcagcgc atagtccgcc
     7801 agcgggtatt tctgaccagt agcgtcgtca gttacaaccg caaacggagt gacttcagaa
     7861 cctgtaccag aagtggtggt gacagcgatc attttcgctt tcacgcccat tttcgggaac
     7921 ttgtagatac gtttacggat atccataaag cgcagcgcca gctcttcgaa gtgagtttcc
     7981 ggatgttcgt acataaccca catgatcttc gcggcgtcca tcggggaacc accacccagc
     8041 gcgataatca cgtctggttt gaaggagttt gccagttctg cacctttacg aacgatgctc
     8101 agggtcgggt ccgcttctac ttcgaagaag acttcagttt caacgcctgc tgctttcagt
     8161 acggaagtga tctgatcagc ataaccattg ttgaacagga agcggtcagt cacgatgagc
     8221 gcacgtttgt ggccatcagt aatcacttca tccagcgcga ttggcaggga gccacggcgg
     8281 aagtagatag atttcggaag tttgtgccac aacatgtttt cagctcgctt agcaacggtt
     8341 ttcttgttga tcaggtgttt cggaccaacg ttttcagaga tggagttacc accccaagaa
     8401 ccacaaccca gagtcaggga aggtgcgagt ttgaagttat acaggtcacc gataccaccc
     8461 tgagacgctg gggtgttaat caggatacga gccgttttca ttttctgacc gaagtaagaa
     8521 acgcgagccg gttggttatc ctggtcagtg tacaggcaag aggtatgacc gataccgccc
     8581 atagcaacca gtttctctgc tttttctacc gcgtcttcga aatctttagc gcggtacatt
     8641 gccagagtcg gggacagttt ttcatgtgcg aacggttcgc tttcatcaac aacggtcact
     8701 tcaccgatca gaatcttggt gttttctggt acagagaagc ctgccagttc agcaatttta
     8761 taggctggct gaccaacgat agccgcgttc agcgcaccgt ttttcaggat aacatcctga
     8821 acagctttca gctctttacc ctgcaacaga tagccgccgt gggttgcaaa acgttcacgt
     8881 acagcgtcat aaacagagtc aacaacaaca acagactgtt cagaagcaca gattacgccg
     8941 ttgtcgaagg ttttggacat cagtacagat gcaactgcac gtttgatatc agcagtttca
     9001 tcgataacaa ctggagtgtt gcccgcgcct acaccgatag ctggtttacc ggagctgtat
     9061 gcggctttaa ccatgcccgg accaccagtc gcgaggatca ggttgatgtc tgggtggtgc
     9121 atcagtgcgt tagacagttc aacagaaggt tgatcgatcc agccgatcag atctttcgga
     9181 gcaccggcag cgatagcagc ctgcagaacg atatcagccg ctttgttggt ggcatctttt
     9241 gcacgcgggt gcggggagaa gataatggcg ttacgggtct tcagactgat cagcgatttg
     9301 aagatagcag ttgaagtcgg gttagtggtc ggaacgatac cgcaaataat accgattggt
     9361 tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca gaacaccaca ggttttttca
     9421 tctttatagg cgttgtagat atattcagaa gcaaagtggt ttttgatcac tttatcttcg
     9481 acgataccca tgccggattc ggcaacggcc attttcgcga gtgggattcg agcatctgca
     9541 gcagccagag cggcggcgcg gaagattttg tctacttgct cttgagtgaa actggcatat
     9601 tcacgctggg ctttttttac acgctctacg agtgcgttaa gttcagcgac attagtaaca
     9661 gccataatgc tctcctgata atgttaaact tttttagtaa atcatctgct cgaatacgag
     9721 agtatagtca gtgcggtgat gatttgctta acctatgaaa atcaaaagct tactcgcgct
     9781 cacactcact gtgatttact aaaagagttt aaacattaga gttattatct ctaatgcgtc
     9841 acttccaggt ggcgtaagca agattactca cttctgggta ctgattacgt gatccaaatc
     9901 aaatttttgc aaagctgaca cctttcagca tcgcttttcg ccattatagc taacagttaa
     9961 taaattgtag tatgatttgg tggctacatt agcatgtttt gcacaactag ataacaataa
    10021 cgaatgatag caattttaag tagttaggag gtgaaaaatg ctgtcaaaag gcgtattgtc
    10081 agcgcgtctt ttcaacctta tttatggcta acattatccg gcttttgctt cggagctaac
    10141 cgtgattcag accttttttg attttcccgt ttacttcaaa tttttcatcg ggttatttgc
    10201 gctggtcaac ccggtaggga ttattcccgt ctttatcagc atgaccagtt atcagacagc
    10261 ggcagcgcga aacaaaacta accttacagc caacctgtct gtggccatta tcttgtggat
    10321 ctcgcttttt ctcggcgaca cgattctgca actttttggt atatcaattg attcgttccg
    10381 tatcgccggg ggcatcctgg tggtgacgat agcgatgtcg atgatcagcg gcaagcttgg
    10441 cgaggataaa cagaacaagc aagaaaaatc agaaaccgcg gtacgtgaaa gcattggtgt
    10501 ggtgccattg gcgttgccgt tgatggcggg gccaggggcg atcagttcta ccatcgtctg
    10561 gggtacgcgt tatcacagca ttagctatct gtttggtttc tttgtggcta ttgcattgtt
    10621 cgctttatgt tgctggggat tgttccgcat ggcaccgtgg ctggtacggg ttttacgcca
    10681 gaccggcatc aacgtgatta cgcgtattat ggggctattg ctgatggcat tggggattga
    10741 atttatcgtt actggtatta aggggatttt ccccggcctg cttaattaat tcctttcaaa
    10801 tgaaacggag ctgccatgct ccgtttactt cgtcattatt tttactttgt tcccgcgcag
    10861 ttatcaaaag caaaaggaat aggtaaaata ttcttctcaa attacag
//
LOCUS       DAPHJE010000135        10872 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPHJE010000135 DAPHJE010000000
VERSION     DAPHJE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.136"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..1708
                     /gene="yeaV"
                     /locus_tag="RH222_003784"
     CDS             98..1708
                     /gene="yeaV"
                     /locus_tag="RH222_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV3798733.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            1740..2864
                     /gene="yeaW"
                     /locus_tag="RH222_003785"
     CDS             1740..2864
                     /gene="yeaW"
                     /locus_tag="RH222_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV3798734.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVQDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            2920..3885
                     /gene="yeaX"
                     /locus_tag="RH222_003786"
     CDS             2920..3885
                     /gene="yeaX"
                     /locus_tag="RH222_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV3798735.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3939..5054)
                     /gene="rnd"
                     /locus_tag="RH222_003787"
     CDS             complement(3939..5054)
                     /gene="rnd"
                     /locus_tag="RH222_003787"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV3798736.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5136..6821)
                     /gene="fadD"
                     /locus_tag="RH222_003788"
     CDS             complement(5136..6821)
                     /gene="fadD"
                     /locus_tag="RH222_003788"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV3798737.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(7026..7607)
                     /gene="yeaY"
                     /locus_tag="RH222_003789"
     CDS             complement(7026..7607)
                     /gene="yeaY"
                     /locus_tag="RH222_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV3798738.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7647..8342)
                     /gene="tsaB"
                     /locus_tag="RH222_003790"
     CDS             complement(7647..8342)
                     /gene="tsaB"
                     /locus_tag="RH222_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV3798739.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8400..10310)
                     /locus_tag="RH222_003791"
     CDS             complement(8400..10310)
                     /locus_tag="RH222_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV3798740.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10442..10786
                     /locus_tag="RH222_003792"
     CDS             10442..10786
                     /locus_tag="RH222_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV3798741.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         2581 a         2926 c         2727 g         2638 t
ORIGIN      
        1 taatcaattt gttaaacaca acatacaact cattaccctg caaataatta caactggcaa
       61 aagccagaga cgggctttta tgcccaggag atgactcatg atgagcaatg ttaagaaaaa
      121 agatgtgccg ctgataagca tcagcctggt ggccattctt ttcatcgcag ctgcattaag
      181 ccttttccca caacaatcgg ccgacgcggc caacgcaata tacacttttg ttacgcgtac
      241 gttaggttcc gccgtacagg tattggtttt gctggcaatg ggactggtga tttatttagc
      301 caccagtaaa tacggcaata ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc
      361 ctggctgttt atgtttattt gtgccggttt aggttcttct acgctttatt ggggggttgc
      421 tgaatgggcc tattattatc aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc
      481 actcgaattt agcgtccctt actccttctt ccactggggc atcagcgcct gggcaactta
      541 tacgctggcc tcattaatca tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag
      601 tctttccggc attattgccg cgattaccgg cgttcgcccg caaggcccat ggggaaaact
      661 ggttgatttg atgttcctga tcgccactgt cggcgcactg actatttccc ttgttgtcac
      721 cgcagcaacc tttactcgtg gactttccgc gctgaccggt ttacccgata acttcaccgt
      781 gcaggcattt gtgatcctgc tttccggcgg cattttttgc ctaagctcgt ggattggtat
      841 caacaacggt ttgcaacgtc tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt
      901 actggtgctg attgtcggcc caaccgaatt tattaccaac agcatcatca atgccatcgg
      961 tctgaccacg caaaacttcc tgcaaatgag cttattcacc gatccgcttg gcgatggttc
     1021 atttacccgc aactggaccg ttttctactg gctgtggtgg atctcataca cccctggcgt
     1081 agcaatgttt gtcacccgcg tttcccgcgg tcgtaagatt aaagaagtta tctggggact
     1141 gatcctcggc agcaccgtcg gttgctggtt cttcttcggc gtaatggaaa gctatgccat
     1201 tcatcagttt atcaatggcg taatcaacgt cccacaggtg ctggaaacac tgggcggcga
     1261 aacagctgta cagcaagttc tgatgtcgtt gccagccggt aaattgttcc tcgccgcata
     1321 cctgggcgtg atgattattt tccttgcctc gcatatggat gcggtggcct acaccatggc
     1381 tgcgaccagt acgcgtaatc tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt
     1441 ctggtgcgtg gtgatcactc tgatcccgct ttccatcttg tttaccggtg cttcgctgga
     1501 aacgatgaaa accaccgtcg tgctcacagc ccttcccttc ctcgtcattt tactggtgaa
     1561 agtcggcgga tttattcgct ggctgaaaca ggattacgcc gacattccgg ctcatcaagt
     1621 tgaacattat ctcccgcaga caccggttga agccctggaa aaaacgccag tgctccctgc
     1681 gggaaccgta ttcaaaggcg acaactgagc gccatcatcc taacgataaa ggtatcccta
     1741 tgagcaatct gagccctgac tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg
     1801 cgtggaccat tcctgcccgt ttttataccg atcagaacgc gtttgaacac gaaaaagaga
     1861 acgtcttcgc caaaagctgg atttgcgtcg ctcacagcag cgaactggcg aatgccaatg
     1921 attatgtgac gcgtgagatc attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg
     1981 ttttgcgcgc gttctataac gtgtgtccgc accgtggtca tcagttgttg agcggtgaag
     2041 gaaaagcaaa aaatgtgatt acctgcccgt atcacgcatg ggcattcaaa ctcgatggca
     2101 atctggccca tgcacgtaac tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac
     2161 tggttccggt gcgtctggaa gaatatgccg gattcgtctt catcaacatg gaccccaacg
     2221 ccaccagcgt agaagatcag ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg
     2281 aagtccaaga tctgaaactg gcggcccgct ttaccacccg cacgcctgcc aactggaaga
     2341 acattgtcga taactatctc gagtgctatc actgtggtcc ggcgcatcca ggtttctccg
     2401 actccgtaca ggttgatcgt tactggcaca ccatgcacgg taactggacg ctgcaatacg
     2461 gtttcgccaa accgtccgaa cagtcgttta aatttgaaga gggtacggat gcggcattcc
     2521 acggtttctg gctgtggccg tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga
     2581 cggtcattta tgaattcccg gtggattctg aaactaccct gcaaaactac gatatttact
     2641 tcaccaatga agagttaacc gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt
     2701 tccgtccgga agatttacgt ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct
     2761 atcgtggtca ggggcgcatc atggccgaca gtagcggtag tggcatttcc gaacatggta
     2821 tcgcccattt ccataatctg ctggcgcagg tgtttaagga ctaatgacat cggcggcggt
     2881 attttccgcc gctgggctga tttttgatgg agtacagcaa tgtcagacta tcaaatgttt
     2941 gaagtacagg tgagtcaggt ggaaccgctt accgaacagg tgaaacgctt cacgctggtg
     3001 gcaaccgatg gcaaaccgct gcctgcgttc accggaggaa gtcacatcat tgtgcagatg
     3061 agcgatggtg ataaccatta cagcaatgcg tattcactac tgagttcgcc gcatgacacc
     3121 tcttgttatc agattgccgt tcggctggag gaaaactcgc gcggcggttc ccgctttttg
     3181 catcagcagg taaaagtggg cgatcggtta acgatttcaa cgcctaataa cctgtttgcg
     3241 ctaattccct cagccagaaa gcatctgttt atcgcgggcg gtattggtat cacccctttc
     3301 ctgtcgcaca tggcagagct gcaacacagc gacgtcgact ggcagctaca ttactgctcg
     3361 cgaaatccag aaagttgcgc atttcgtgat gagctagtcc agcatccgca ggctgagaaa
     3421 gttcacttgc atcactcatc aaccggaaca cgactggaat tagcgcgatt attggcggat
     3481 atcgaacctg gcacacacgt ttatacctgt ggtcccgagg cgctaaatga agcggtaaga
     3541 agtgaagctg cgcgtctgga catcgtcgcc gatacgctgc actttgagca atttgctatc
     3601 gaagacaaaa ccggcgatgc ctttaccctg gtgcttgccc gttccggaaa agagtttgtg
     3661 gtgccggaag agatgactat tttgcaggtt attgaaaata ataaagccgc gaaagtggaa
     3721 tgtttatgtc gtgaaggggt atgcggaacc tgcgaaacag caatactgga aggtgaagct
     3781 gaccatcggg atcagtattt tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc
     3841 tgttgttcgc gtgcgaaggg taaacgcctg gtgttggatt tgtagtttgc caaagccgga
     3901 tgtggcgctg agcgcgccca gtccggcttc ggaagatttt actgcggata ttcctgcaat
     3961 aaattgtgta atgcttccgc catcagctca ccacgccagc cggaaatcag ctccggcaaa
     4021 ttgttctgcg gtttcagttt ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat
     4081 gccagcaatt cggcgctgat cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc
     4141 gctttaaaca ctttacgata accaggcatg tccatcaggt taagcatcgg ctgcggtaag
     4201 gcctcttccg gcaatgtctg cgctttttcc accagcgcca gcagcgtttt accgtgaaag
     4261 cggatttcgc taccggataa acccaggctg tccagttcgc ctaaacttcc cggcatataa
     4321 cgcgcgaccg accacaaatg ctcttcacgc acgacaaagt tcaccgccag atcacgctct
     4381 cgcgccttgc gcagtcgcca gtcggctaac agttgcagac aggccagttg gcgtgtgcgt
     4441 aactgccagg cattggtgat atcacgccag gcatcttccg gcgcaacgac ttcctgacga
     4501 cgcatttgca tcaggcggca ttcatccagc gccgcaggta gccagccgga ggcctccgtt
     4561 tctaccataa gcttggcggt gatcggtaac agataccaga catccgccgc tgcgtattca
     4621 cactgacgtt cggtcagcgg tctggccagc cagtcggtgc gcgattcact cttgtccagc
     4681 gtaacgccgg aatactcttc caccatggaa gcgaaacccc atgacatcgg gcgtccgcag
     4741 aaggcagcaa ggatttgcgt gtcaatcaag ggttgtggta attcgccaaa gacattgagg
     4801 aacacttcca gatcttcact gcctgcatgg agaaattttg tgatggacgg atcgcgcagg
     4861 atcgctttca gcggtgacca gtcggtgatc ccgagaggat caattagcgc cagatgctcg
     4921 ccatcgaaaa gttgaatcaa ccccagctgc gggtaataag tgcgcgtacg aacaaattca
     4981 gtatccaggg ctatcgccgg aaaggcacgg acggcttcac acaaagaagc cagcgcatcg
     5041 tccgtggtaa tcatttggta attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg
     5101 ccggattaac cggcgtctga cgactgactt aacactcagg ctttattgtc cactttgccg
     5161 cgcgcttcgt cacgtaattc tcgtcgcaaa atttttccga cgttagattt cggtaactca
     5221 tcacgaaact ccaccagctt cggtactttg tagcccgtga gctgacggcg gcaaaaggtc
     5281 accagtgact cttcggtaag cgatggatct tttttcacta cgaagatttt caccgcttca
     5341 ccactggagc cggaaggtac gccaacagcc gcgacttcct gtacgccagg atgctgcatg
     5401 acgacatctt caatctcgtt gggatagacg ttaaaaccgg aaaccagaat catgtctttt
     5461 ttacgatcga caatgcgcag gaacccttct tcatccatca ccgcgatgtc gccggtgtgt
     5521 aaccagccat ttttgatgat ctcatctgta gcatccggac gctgccagta acccagcatc
     5581 acctgcggtc ctttgacaca aagctcaccc ggttgacccg gtggtacttc attatcatca
     5641 tcatccacca gtttggcttc cgtcgacggc accggcaaac cgatgctacc actatgataa
     5701 tcaatatcat atgggttaac gctgaccagc ggcgcacact cggtaaggcc atagccttcc
     5761 agcagatact gtcctgtcag tttcacccaa cgctctgcca ccacttgctg cactggcatc
     5821 cctccgcctg cggaaagatg cagactggag aaatccagct gctggaactc tttattgttc
     5881 agcaacgcat tgaacaaggt gttaacgccc gtgatagcgg taaacggata tttcgctaac
     5941 tcttttacca accctggaat atcgcgcggg ttagtgataa gcaggttctg cccacccagt
     6001 tcgataaaca gcaggcagtt aatggtcagg gcaaaaatgt gatacagcgg cagtgccgtt
     6061 actaccagtt ctttacctgg atgcagcaac ggcccatagg tggcgttgac ctgttccagg
     6121 ttcgccagca tattgcggtg agtcagcatc gcgcctttcg ccacacctgt ggtgccgccg
     6181 gtgtattgca ggaaggctaa atcttccggc accagttcgg gtttgacgta ctgcatccgg
     6241 tagccgttat gcagtgcgct acgaaatgaa atggcatctg gcagatggta tttcggcacc
     6301 aaacgcttga tgtatttaac aacgaaattg actaccgtgc cttttgccgt agatagctga
     6361 tcgcccatac gggtcagaat tacgtgctga acggcggttt tatcaaccac tttttccagt
     6421 gtgtgagcaa agttagacac gataacaatc gccgatgcgc cgctatcgtt aagctgatgc
     6481 tcaagctcac gcggggtata caacgggtta acgtttacga cgatcatccc ggcacgcaaa
     6541 atgccaaaca gcgccaccgg atattgcaat aaattaggca tcatcaacgc aacgcgatcg
     6601 cctttcttca gccccaaccc ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc
     6661 agcttgcgga aggtcattac ctcccccata ttcacaaacg caggctgatc ggcgtagcgc
     6721 gcgaccgact gctcaaacat atctaccaga gattgataac ggtcagggtt gatctccgtc
     6781 ggaacgtccg cgggataacg gttaagccaa accttcttca attcttcacc tctaaaatgc
     6841 gtgttcgtcg tcatcgcaac cccaaatgat atacatgccg ttaacataat attaactcat
     6901 cataccagct tgataattac ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta
     6961 tctttaccgt aagaatgcag aaacagcgga ccagccgctg tttctttttc tttaaaaaca
     7021 agcaattatt cagttacaac tgtttgtact ctcgcggggc cgggattata ccagccccat
     7081 ccgccatagc catagggcca gccacgaccg ccataaaacc atggatcaat cggctgaggc
     7141 ggcataatca cctgctgggt taaatgccaa cgtttgtaac ccgttacttg catcaccata
     7201 aatttatagg gcgtgttgcc gatttttccg tcaaccgcac cagtgattgg cccgactacc
     7261 gtaaccagtt gtccacggaa atccaccggg tccagaaaac cgttcatatc ggcataaatg
     7321 cgaccgcgag aaggttctcc cagcgtcggt cttgctccgc tgtccagcgg tacggtagca
     7381 atttccaggc gggttttccc ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt
     7441 gcctcctgac caacgtacag ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc
     7501 gtgggactgc tgcctttaat ggcgtccggc acagtgacac aaccgctcag cattagcgca
     7561 aacgtacctg ccagtatgcc tttgataaca ttcttttgaa ccgccatgat gcgactcctt
     7621 tttttctcag ggcatactct taagattcat tctttgcccg gaagtttctt ccatgcgacg
     7681 ttgttacgta aataaaccgg ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac
     7741 atctgacacg caatcggcag catatcttcc gccgctggca gtaaaacttc tccgtcgcgc
     7801 aaaaccagcc cgctctcttt accgagatcc ggccaggctt gccagcccgt ccctaccgtt
     7861 acccattcgc cggaaagctg ttgcattcgt tcatgaacga gttcgggttt gagtacggct
     7921 tcggtttctt caccgcgcca gataccgttt tcatcacgct gatattcggc ccagtaaact
     7981 tcgcccattc gcgcgtcaat ggctgccagc acgcgggttg cgccgttttt gcgccacgcc
     8041 ccttgcgcca tcgtcattag cgtggagacg ccaatcatcg gtagctctgc gccaagtgcc
     8101 agcccttgcg caatgccaat accaatgcgc acgccggtaa agctaccggg gccgcgcccg
     8161 taagccagag cgttaatatc agtcagggaa gttccgctgg tggtcaggat atcctgcacc
     8221 atcggtaaga ttcgttgagt atgttcacga gggcaaagct caaaatgagc gttgacagta
     8281 ccgtcgttcc acagggcgac agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc
     8341 atgggtcttc gtgcttagat caataaaaag gcgcgcatca taccatactc cgtaacaaat
     8401 tacctggagg atggtatcgc aaggaaacga accgcacggg caatgtcacg agtgcgtggc
     8461 gcgggcggca gactggcgag aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca
     8521 caaatcacca gcacgccacg atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg
     8581 agagtaatga cggcatctgg tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga
     8641 caatcttcca tgcgcgcttt taacagtgga tcatccggcg aggtaaacgg caacttgtcg
     8701 ataatcacca atgacaatgt atcgccacgc acgtccaccc cttcccagaa actactggtt
     8761 gccacaagaa gcgcgttacc ggcgctgaca aattgctgca acagttgccc tttgctggtt
     8821 tccccctgca acaaaacggg aagcgtcatg gtagcgcgga actgctcggc cagatcacgc
     8881 atcatggcgt gcgaggtaca aagcataaaa caacgaccgt tgttagcttc gatgatcggt
     8941 cgcagcattg ccgctaactg gcgagcagaa cctggctggt tggtttgcgg cagattgcgc
     9001 ggcacacaga gtaacgcctg gcggctgtaa tcaaacgggc ttggcaatag caacgactcc
     9061 gcctgttcta tcccaagccg cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt
     9121 gctgaggtga agatccagct accaggcttt tgcgccatta actctttgaa tttatccgcc
     9181 acgctgagcg gcgtgagagc cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg
     9241 tagcccggct gattgatctc ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc
     9301 tcaaatgccg catccagcaa ggcggaacgc cccagtgaca gtttcgccac gtcataacaa
     9361 agttccaggg tgtcatcgag cagtaaaaat gcccgctgaa tttgcggatt agctaacagc
     9421 tcacgcaggt taccgcgata acctggctca ccgagttgca gacgaaaatc ctgcgcactc
     9481 tgggcaagac gatcagcgca cttttgtaac tgctgggtgt cttttaattc ggtgcggtaa
     9541 gcgatggtga tgtcttttgc caggtcgagc agttgtcgac tggagagtga ctgaccaaaa
     9601 tactggctgg caatgtccgg tagctggtgg gcttcgtcga agatcatgac gtccgcttcc
     9661 gggatcagtt cgccaaatcc actctcttta accaccatat ccgccagaaa gagatgatgg
     9721 tttaccacca ccacatcggc gtccatcgct tttttacgtg ctttgaccac aaagcaatct
     9781 ttatacatcg ggcagtcgct gccaagacag ttgtcgttgg tgctggtgac cagcggccac
     9841 gcctgtgaat cttccgccac gctgacgcag gtgctgatat caccatcgac tgtttgatta
     9901 gaccaggagc gcagcaggat cacatcgctt aagatttgta ccggcagatc gccccccgcc
     9961 agcgcctgct gttcgagacg ctcgaggcag aggtagtttg agcgcccttt cagcagcgcc
    10021 acgttgcccg tatatttcaa tgcctttgag actgttggca aatcgcggct gtagagctga
    10081 tcctgcaacg cttttgagcc ggtcgagata atgactttct ttttcgcccg cagcgcagga
    10141 gccaggtaag cgtaggtttt gcccgtaccg gttcctgctt ccaccaccag cggctggcct
    10201 ttttctatcg cctgggtgac ggctaccgcc atctgtcgct gtggttctcg cggcttaaag
    10261 cctggtatcg ctttcgccag ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct
    10321 gttgatttga acagggatta tgtcaggatg agggcgcttt cgccagttga agtggtgacg
    10381 gcgacctcac attgtggcag tctttgcagc acgaaaatgg aaggataatg aggacaaaat
    10441 gatgactatc gttcgtatcg atgctgaagc ccgctggtct gatgtggtaa tccacaacaa
    10501 cacgctctac tacactggtg taccggaaaa cctcgacgcc gatgcctttg agcaaaccgc
    10561 caacacgctg gcgcagattg atgccgtgct ggaaaaacag ggcagcaata aatcgagcat
    10621 tctggatgcc accattttcc tggccgataa aaacgacttc gcggcgatga ataaagcgtg
    10681 ggatgcctgg gttgtcgcgg gtcatgcgcc ggtgcgctgc acggtacaag cgggtttgat
    10741 gaacccgaag tataaagttg aaattaagat tgtggctgcg gtgtaagctt tatcgaagca
    10801 aaataagtca gacgataatt tatcgataat actggtcggt tttacataaa tcgaccagag
    10861 attaagatat ta
//
LOCUS       DAPHJE010000136        10799 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPHJE010000136 DAPHJE010000000
VERSION     DAPHJE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.137"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            71..112
                     /locus_tag="RH222_003793"
     CDS             71..112
                     /locus_tag="RH222_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798742.1"
                     /translation="MVQLVDLARCVSF"
     gene            150..1532
                     /gene="dosC"
                     /locus_tag="RH222_003794"
     CDS             150..1532
                     /gene="dosC"
                     /locus_tag="RH222_003794"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV3798743.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1557..3956
                     /gene="dosP"
                     /locus_tag="RH222_003795"
                     /gene_synonym="pdeO"
     CDS             1557..3956
                     /gene="dosP"
                     /locus_tag="RH222_003795"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV3798744.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKETISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4214..4795
                     /gene="ddpX"
                     /locus_tag="RH222_003796"
     CDS             4214..4795
                     /gene="ddpX"
                     /locus_tag="RH222_003796"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV3798745.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQVCPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHDRSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            4809..6359
                     /locus_tag="RH222_003797"
     CDS             4809..6359
                     /locus_tag="RH222_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3798746.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTSVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6361..7383
                     /locus_tag="RH222_003798"
     CDS             6361..7383
                     /locus_tag="RH222_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3798747.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQHLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFSATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLSGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7380..8276
                     /locus_tag="RH222_003799"
     CDS             7380..8276
                     /locus_tag="RH222_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV3798748.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8273..9259
                     /locus_tag="RH222_003800"
     CDS             8273..9259
                     /locus_tag="RH222_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3798749.1"
                     /translation="MTQPVLDIQQLHLSFPGFHGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9252..10178
                     /locus_tag="RH222_003801"
     CDS             9252..10178
                     /locus_tag="RH222_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3798750.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRV
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCKVRQSLAIREDGRELRCWRAL"
     gene            complement(10234..10665)
                     /gene="osmC"
                     /locus_tag="RH222_003802"
     CDS             complement(10234..10665)
                     /gene="osmC"
                     /locus_tag="RH222_003802"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV3798751.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2621 a         2685 c         2799 g         2694 t
ORIGIN      
        1 cagtagcgta gattaaaact gtcctaagtc gcttatttct tttcagtata tcttcatatt
       61 tcaggagaat atggtgcagt tagtcgatct cgcacgttgc gtttcgtttt gattccgtct
      121 gatttcccag ccttataagg gtgtgaatta tggagatgta ttttaaaaga atgaaagatg
      181 agtggaccgg acttgtcgaa caggcagatc ctctcattcg tgctaaagcc gcagaaattg
      241 ccgttgcgca tgctcattat ctgagtatcg agttttatcg aattgtccgc atcgacccgc
      301 atgccgaaga attcttgagt aatgaacaag ttgagcggca gttgaaaagt gcgatggaac
      361 gctggattat taacgtgctt tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc
      421 agcataccgt cgcggaagtg catgcccgca taggaattcc ggtagaaatt gtcgagatgg
      481 ggtttcgggt gctgaaaaaa atcctctatc cggtaatctt ctcttcggat tattccgccg
      541 cagaaaaact tcaggtctat catttctcga ttaacagtat tgatatcgcg atggaagtga
      601 tgacccgcgc gtttaccttt agtgacagta gtgcctcaaa ggaagatgaa aactatcgta
      661 tcttctcgtt gctggaaaac gccgaagaag aaaaagaacg gcaaatagcc tcaatacttt
      721 catgggaaat agatattatc tataaaatcc tgctggattc tgatttaggc agtagtttgc
      781 ctttaagcca ggctgatttt ggcctgtggt ttaaccataa aggtcgacat tattttagtg
      841 gcattgccga agtaggccat atctcccgtc tgattcagga tttcgacggt atattcaatc
      901 aaaccatgcg taacaccagg aatctgaata acagaagtct gcgggtgaaa tttttattac
      961 agataagaaa tactgtatcg caaattatta ccttgctgcg tgaattgttt gaagaagtat
     1021 cgcgccacga agtcggtatg gatgtactga cgaaattact taaccgccgt ttcctgccga
     1081 ctatcttcaa acgcgaaatt gcccatgcca accggaccgg tacaccgctg tcagtgctga
     1141 ttattgacgt tgataaattc aaagagatca acgatacgtg gggccataac actggcgatg
     1201 aaattctgcg taaagtctct caggcctttt atgacaacgt ccgcagtagt gattatgttt
     1261 tccgctacgg gggcgatgaa tttatcattg ttttgactga agcttcggaa aacgaaacgt
     1321 tacgtaccgc agaacgtatt cgcagtcggg tggagaaaac caaactgaaa gccgcaaacg
     1381 gcgaggatat tgccctctca ctttccatcg gtgccgccat gtttaatggt catcctgact
     1441 atgagcgcct cattcaaata gccgatgaag ctctgtatat cgccaaaaga cgaggtagaa
     1501 accgtgttga actctggaaa gccagtcttt agatgcgcca ggatgcagag gtaatcatga
     1561 agctaaccga tgcggaaaat gccgccgatg gcattttttt ccccgccctt gagcaaaata
     1621 tgatgggtgc ggtgttaatt aacgaaaatg atgaagtgat gtttttcaac cccgccgcag
     1681 agaagctctg ggggtacaaa cgtgaagaag tcattggcaa taacattgat atgctgattc
     1741 cgcgggattt gcgtcctgcg catcctgaat acattcgtca taaccgtgaa ggcggtaaag
     1801 cgcgcgtcga ggggatgagt cgggagctgc agctggagaa aaaagacggc agtaaaatct
     1861 ggacccgttt tgcgctatcg aaagtgagcg ccgaggggaa agtttattac ctggcgctgg
     1921 tacgggatgc cagcgtagaa atggcgcaaa aagaacagac ccgacaattg attattgccg
     1981 ttgaccatct cgaccgaccg gtgattgtcc tcgatccgga acgccatatt gtgcagtgca
     2041 atcgcgcatt taccgaaatg tttggttact gcattagcga agccagcggt atgcagcccg
     2101 atacactcct gaacattcct gaattccctg ccgataaccg cattcgttta caacagttgc
     2161 tatggaaaac cgcccgcgat caggacgaat ttctgctgtt gacgcgcacc ggtgaaaaaa
     2221 tctggattaa agcctctatc agcccggttt atgacgtgct cgcgcatctg cagaacctgg
     2281 taatgacttt ctcggatatc accgaagaac ggcagattcg ccagcttgaa ggcaatattc
     2341 tcgccgccat gtgcagcagc ccgccatttc atgaaatggg ggaaatcatt tgtcgtaaca
     2401 tcgaatctgt actcaacgaa tcgcatgttt cgctgttcgc actgcgcaac gggatgccga
     2461 tacactgggc gtcatcttcg cacggtgcag aagttcaaaa tgcgcaaagc tggtcagcga
     2521 ccattcgtca gcgtgatggc gcgcctgcgg ggatcctgca aattaaaacc tcgtcaggag
     2581 cagaaaccag cgcctttatc gaacgcgtgg cagatatcag ccagcatatg gccgcgctgg
     2641 cgctggaaca ggaaaaaagc cgtcagcata ttgaacaact catccaattt gatccgatga
     2701 ccggtctgcc aaatcgcaat aacctgcaca attacctcga tgacctggtc gacaaagccg
     2761 tctctccggt ggtgtatctc atcggtgttg accatattca ggatgtgatt gatagccttg
     2821 gctatgcgtg ggccgatcag gcattgctgg aagtggtcaa tcgctttcgt gaaaaactca
     2881 aaccggatca gtatctctgt cgtatcgaag gtacgcagtt tgtcctcgtg agcctggaaa
     2941 acgacgtcag taacattacc cagatcgccg atgagctacg gaatgtggtc agcaagccga
     3001 taatgattga cgataaaccc ttcccgctta ccttgagtat tggcatcagc tacgacgtgg
     3061 ggaaaaaccg cgattacctg ctctccactg cccataacgc gatggattat attcgcaaga
     3121 atggcggtaa cggctggcag ttcttcagcc cggcgatgaa cgaaatggta aaagagcgtt
     3181 tggttttagg cgcagcgctg aaagaaacga ttagcaataa ccaactgaaa ctggtttacc
     3241 agccgcaaat cttcgcagaa acgggtgaac tgtacggcat cgaagccctt gctcgctggc
     3301 acgatcccca gcatggtcat gtaccccctt cacggtttat tcctctcgca gaagagattg
     3361 gtgaaatcga aaatattggg cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat
     3421 ggcgtagcca gaatattcat atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc
     3481 gcagtaatca actgcctaat caggtgtctg atgcaatgca agcctggggt attgacggcc
     3541 accagctgac ggtggaaatc acggaaagca tgatgatgga acacgatacc gaaatcttta
     3601 agcgcattca gatcctgcgt gatatgggcg tgggtttatc ggtagatgat tttggcacgg
     3661 gcttttccgg attatcccgc ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa
     3721 gttttgtcga tcgttgtctg acggaaaaac gcatccttgc cttacttgaa gccattacca
     3781 gcattgggca aagcctcaat ttaaccgtcg tggcggaagg cgtcgaaacc aaagagcaat
     3841 ttgagatgct acgcaagatc cactgtcgcg ttattcaggg atatttcttt tcccgccccc
     3901 tgcccgccga agaaattcca ggctggatga gcagcgtgtt accgctgaaa atctgacaaa
     3961 ttcctctcgc ccgcactcgc gggtattcat ttaacgtgac actgtcactt aaatactgtg
     4021 atttcagcta cgattccggg agattccttc cttaacttcc cctcttgcac caaaactgca
     4081 ctacgctgca caaatgttga tcaaaagtta aaaaatataa ctttaagcca gcattctggc
     4141 gcttctcccg gcatggcatg agatctgcat aagcggaaag cgcagcaatt tttgtcttat
     4201 acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtaa tcttccctga
     4261 tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag ctatttatca
     4321 gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa gtatcagtat
     4381 cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac aacaagcaca
     4441 ggcgatgttg tggcaagtct gcccagaccc gcaatatgtt gttgatgtga cggtcggttc
     4501 taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg ggaacatcct
     4561 cgatatgggc gcagggttcg atgaaatgca cgatcgctcc catgcttacc atccttccgt
     4621 cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg attatgactg gtggcggttt
     4681 tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga gttacccact
     4741 gctcgctgat caattcacct gttttatatc ccctggcaca cagcacgtca gttaatcagg
     4801 aaactgtcat gaaaagatcg atatcgtttc gtcctacatt gctcgcgctc gtccttgcca
     4861 caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatc ggtaaggctg
     4921 ccgatccaca aaccctcgac ccggcggtga caattgataa taacgactgg acggtgacct
     4981 acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc tcaaccgacg
     5041 ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag tggacgttca
     5101 ccctgaagga taatgctaaa tttgccgatg gcacatctgt cactgccgaa gcagtaaaac
     5161 tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt cccaaagatt
     5221 taaagattga tgctcccgac gaacatacgg tgaagtttac ccttagccaa ccattcgcac
     5281 cgttcctcta cacgctggcg aatgacggtg catccattat caatccggcg gtgttaaagg
     5341 aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt tccggaccat
     5401 ttatgctgaa aagctggcaa aaaggtcagc aattagttct ggtgccaaat ccgcattact
     5461 ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa agtgcctccc
     5521 gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg gtggatcaac
     5581 tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca ctgcgcgtca
     5641 cctatctgta tctgaataac agcaaagcgc cacttaatca ggcggatctg cgtcgggcca
     5701 tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt aacggaaaac
     5761 aaatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca atgcaataca
     5821 accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa cccaccagcc
     5881 tgacgtttct ctactccgat aacgatccga actgggaacc gatcgctctg gcgacacaat
     5941 ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac gccaccatgc
     6001 gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt ccggattttg
     6061 ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt ctgccgggta
     6121 accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg cttgcgacca
     6181 ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc attgatgacg
     6241 ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa gaggtgaaag
     6301 gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg agtaaataac
     6361 gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt ggtggcgggc
     6421 gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gtgatccggc gcggttactg
     6481 gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcatct gggactggac
     6541 cagccactgt acgtacagtt ttaccgctac gtcagcgacc tgtttcatgg tgacctcgga
     6601 acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt ctcggcgacg
     6661 ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcattcc gctgggcata
     6721 ctctctgccg tctggcgaaa tcgctggctg gatcatctgg tgcgaataat ggccattacc
     6781 ggaatctcca cacctgcgtt ctggcttggg ctgggcgtca ttgtgctgtt ttatggtcat
     6841 ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccgcc aacgcacgtt
     6901 accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt cttcaatgcg
     6961 ttgcaacatc tcatcttacc ggcattaacg ctggcgtttg ttcacctggg gattgtcgcc
     7021 cgccagatcc gttcagcgat gctggaacaa ttgagtgaag actacattcg taccgccagg
     7081 gccagcggct tgtccggctg gtatatcgtt ttatgttatg cgctacccaa tgcgttgatc
     7141 ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc agtgctcacc
     7201 gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag tgacatcaat acaggcgctc
     7261 gacttcccgg ctgtgatggg ctttgccgtc gtggtttcat ttgcttatgt gctggtcaac
     7321 ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg aggtggtgaa
     7381 tgatgctaag cgaggaaacg tccgccgtac gtccacaaaa acaaacgcga tttaatggtg
     7441 caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg
     7501 tattaatgct actgatgatg attttttcac cgtggctggc gacgcatgat cccaacgcca
     7561 ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc accgatgaag
     7621 tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat
     7681 tagtggtggt cgccattgcg ggtatgattg gttcgctact cggatgtcta tccggtgtgc
     7741 ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg tcgattcctt
     7801 cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttgttt aacgccatgc
     7861 tggctattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg caagcattag
     7921 ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggcgcgtct cgttggcatc
     7981 tgatcaactg gcatatttta cgtaactccc taccgccgct gatcgtgcag gcatcgctgg
     8041 atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg ggtgctcaac
     8101 aaccgagtgc tgaatggggc gcgatggtag caaatggtcg caactatgta ctcgatcagt
     8161 ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg tttaatctct
     8221 ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca
     8281 acccgttctg gacattcaac aactgcattt gagtttcccc ggttttcacg gtgatgttca
     8341 cgcgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc tggtgggaga
     8401 atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac cgacgggcag
     8461 ttattgcgta catcgggggc agatttccct gctgggagaa gatgttctta acgcgcggga
     8521 aaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg aaccgatgac
     8581 cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc gccatcatca
     8641 accaataagt cgtcgggaag ccagagctaa agcgattgac ctgctggaag agatgcaaat
     8701 cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca
     8761 gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg ccgacgaacc
     8821 gactacggcg ctggacgtca cggtgcagtt gcaggtactg cgtttgctta aacataaagc
     8881 ccgcgccagt ggaactgcgg ttctgttcat cagccatgat atggccgtgg tgtcgcaact
     8941 gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg tgacggcaga
     9001 cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg caccagaaca
     9061 tggagtacca cgccagctat tacccgccat tccagggacg gtaccaaacc tcacccattt
     9121 gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt gtgaaaacgt
     9181 cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt atccgcagca
     9241 ggaggtcata agtgtctgac acgttattaa cgttacgcga cgtccatatc aatttcccgg
     9301 cccgtaaaaa ctggcttggt aaaactacgg aacatgttca tgccattaat ggtattgatt
     9361 tacagatccg ccgtggtgaa accttaggga tcgtcggcga gtcaggctgc ggcaaaagca
     9421 ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcagtac atccgttctg
     9481 gctcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt aatccgcgct
     9541 taccggtgtg gcgcgtcatc acagaaccgc tctggatagc taagcgtagt agtgaacaac
     9601 agcggcgagc gttggcagag gagttggctg tgcaggtggg tattcgtccg gagtatctcg
     9661 accgcctgcc tcatgcgttc tccggcgggc agcggcaacg tatcgccatt gccagagcac
     9721 tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctctgcgctg gatatctccg
     9781 ttcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg ctgacctatg
     9841 tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg gcggtgatgt
     9901 atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca cctgcacatc
     9961 catacacccg attattgctg gattccctcc ccgccattga taaaccgctg gaggaagaat
    10021 gggcattacg taaaacggat ctgccgggaa accgcacgtt gccgcaaggc tgttttttct
    10081 acgaacgttg cccgctggca acccacggat gtaaagtccg gcaatcatta gcaataaggg
    10141 aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa cgcatcaggc
    10201 attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt aatccagcgt
    10261 aatttccgct ttcagtacct gagagaccgg gcatcctgct tttgctttct ggatgatgcc
    10321 gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg cgattttcgt
    10381 aatcgcaaaa ccggcatcca ctttatccag cgacacatcg gcggtggtat caatcgatgt
    10441 tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac atgcggcatg
    10501 cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa
    10561 tccatacggc tgttggttca gcacgccact ctcggtggat actgttccct tcccgcgttt
    10621 gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg ctctcctgtg
    10681 ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc aggatattcc
    10741 gaataaaatc cggcgaaaat attacttctg cacttaaatt aaattcccac aatagatta
//
LOCUS       DAPHJE010000137        10766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPHJE010000137 DAPHJE010000000
VERSION     DAPHJE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.138"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            147..860
                     /locus_tag="RH222_003803"
     CDS             147..860
                     /locus_tag="RH222_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV3798752.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            892..1650
                     /locus_tag="RH222_003804"
     CDS             892..1650
                     /locus_tag="RH222_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798753.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1696..3045
                     /locus_tag="RH222_003805"
     CDS             1696..3045
                     /locus_tag="RH222_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV3798754.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPHQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3045..3869
                     /locus_tag="RH222_003806"
     CDS             3045..3869
                     /locus_tag="RH222_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798755.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3881..4441
                     /locus_tag="RH222_003807"
     CDS             3881..4441
                     /locus_tag="RH222_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV3798756.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4472..5542
                     /gene="lptF"
                     /locus_tag="RH222_003808"
     CDS             4472..5542
                     /gene="lptF"
                     /locus_tag="RH222_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV3798757.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5539..6618
                     /gene="lptG"
                     /locus_tag="RH222_003809"
     CDS             5539..6618
                     /gene="lptG"
                     /locus_tag="RH222_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV3798758.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6654..7826)
                     /locus_tag="RH222_003810"
     CDS             complement(6654..7826)
                     /locus_tag="RH222_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3798759.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7826..8074)
                     /locus_tag="RH222_003811"
     CDS             complement(7826..8074)
                     /locus_tag="RH222_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3798760.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8106..9020)
                     /locus_tag="RH222_003812"
     CDS             complement(8106..9020)
                     /locus_tag="RH222_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV3798761.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9017..10747)
                     /locus_tag="RH222_003813"
     CDS             complement(9017..10747)
                     /locus_tag="RH222_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV3798762.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIELLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         2585 a         2677 c         2819 g         2685 t
ORIGIN      
        1 ttaaaaatat ttatttggtt aatatgtttt tatgaaagcg taattcaggt caatgtcaca
       61 attaaccatt gtcacaataa ggttgaacgg atattctgag cacggaccac gtaatattca
      121 atacattatt tactgccggg tttttcgtga atcaacgtaa tatgtcaata attaattcca
      181 cgccagtgca tgttattgcc attgtaggat gtgatggttc aggtaaatcg accctcacgg
      241 caagcctggt aaatgaactg gcagcaagaa tgccaacaga acacatttat ctcgggcaat
      301 cgtccgggcg aattggcgaa tggatttcac agctccctgt tattggcgca ccttttgggc
      361 gttatctgcg aagtaaagcg gcacatgtgc acgaaaagcc ctcaacaccg cctggcaata
      421 ttactgcact ggttatctat ctgctttcct gctggcgggc gtacaagttt cgcaaaatgt
      481 tgtgtaaaag ccagcaaggc tttctgctca tcaccgaccg ctacccgcag gttgaagtgc
      541 cggggtttcg ctttgatggc ccgcaattgg caaaaaccac gggcggtaac ggttggataa
      601 aaatgttaag gcagcgcgag ctgaagctgt accaatggat ggcatcttat ttgcccgtat
      661 tgttgattcg tcttggcatt gatgaacaaa cggcgtttgc gcgtaaacct gaccaccaac
      721 tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact gacattcaat ggtgcaaaga
      781 tccttgaact ggatggtcgg caaccagccg atgaaattct gcaagcgtca ctacgcgcaa
      841 ttcacgccgc gctttcctga tcaccaaaat ggatagggga tgatttaacg aatggatgca
      901 ctacaaacac aaactgttat tagtacaacc gcgccgcagc ccagctacat tccggggcta
      961 attgcggtgg tcgggtgtga tggcaccggt aaatccacac tgaccaccga cctggtgaaa
     1021 tcgctgcaac aacactggca aaccgagcgg cgctatctgg ggctgctctc cggcgaagac
     1081 ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg actggcggcc
     1141 aaatcctcga aaacccaaag catgaaaacc aaatctccgg cgctatgggc ggcggtgatt
     1201 atgtactgct tctcgctgcg aagaatggcg aatctacgca aggttcagcg actggcgcaa
     1261 agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg cttttattat
     1321 gacggaccgg ggattggcgt cgaacgtgcg accgggaaaa tcagcatgtt tctggcgcag
     1381 cgcgaacggc gtttatacca acaaatggcg caatatcgcc cggaattaat tattcgcctg
     1441 ggcattgata ttgagactgc catctcccgc aagcctgacc atgactatgc cgagctgcag
     1501 gacaaaatcg gtgtcatgtc gaagattggc tataacggca caaaaattct tgagattgat
     1561 tccagagcgc cctacagcga agtgctggaa caggcacaga aggcggtttc tctggttgcc
     1621 atcgtttctg accgccgaag tttaacttaa ggctggcggg atacttttta ctatcaacga
     1681 taaggtcagg ctgaattggc gggttttaac atcaaacatt ggtttgcaga tggcgcgttt
     1741 cgcaccatta ttcgcaatag cgcctggtta ggctccagta atgtcgtgag cgccttgctg
     1801 gggctgttgg cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt tggcgtactg
     1861 gtgattgtgc aatcgtacgc caagtcgatc agcgatttta ttaagtttca gacatggcag
     1921 ctggtggttc agtacggaac gccagccttg accaacaata atccgcacca atttcgcaat
     1981 gtcgtctcat tttccttctc gctggatatc gtcagcggcg cggtggcgat tgtcggtggc
     2041 attgccttac tgccattcct ttcccattca ttaggtctgg atgaccaaag tttttggctg
     2101 gcagcgctct attgcacgct cattccttca atggcttcct ccacgccgac cggcattctg
     2161 cgtgcggtag atcgcttcga tttaattgct gtacagcagg cgacgaaacc ttttctgcgc
     2221 gcagcgggga gcgtcgtagc ctggtatttt gactttggtt ttgcgggttt tgttattgcc
     2281 tggtacgtgt cgaatctggt tggcggcacc atgtactggt ggtttgccgc gcgcgaatta
     2341 cgccgccgaa atatccataa tgccttcaaa ttgaatctgt ttgagtctgc ccgacacatc
     2401 aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc actccatctg gtcggcgcgt
     2461 aactcgtgca gcacagtgct ggtggggatc gtgttaggac ccgctgccgc cgggttattt
     2521 aaaatcgcca tgacattctt cgacgccgcc ggaacgccag cgggtttgct gggtaaaagt
     2581 ttttacccag aggtgatgcg tttagatccg cgcaccacca gaccgtggtt gttaggtgtg
     2641 aagtccggtt tactggcggg cggaatcggt attctggtgg cgctcgcggt gtttatcgtc
     2701 ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta tgacctgatt
     2761 caggtgatgc tgggcgcaat tgtgatttcg atgctgggct tccctcagga gtcattgcta
     2821 ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc aatcggctac
     2881 atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc tgggagcagc gtttgcttac
     2941 ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc caaccttaaa ggcatttttt
     3001 cagcgccatt ccttgcttta taacgcagcc ggagagaaat cctgatgatg agaaaatatt
     3061 tcccgcttga ggcgagcgag cgtctatttg ttgcgattga agaagatgat gtcgttgatg
     3121 cacaggttag tttgccgccg actattgcac taagttgtac caccgaaatc attcatgata
     3181 attacgcgtt atgcctgcaa ttctggctca atggcgttga tcgtcaggaa ttactgcgtt
     3241 tagttcgcaa acaagccaaa ggcgatgagc tgactgctga tgagcgcaaa caatttaaat
     3301 acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg gctgtattta aagaagcatc
     3361 aggccggatt tttattcggc aaaacgaccg tttttcttgg gcgttttcag gacggctttc
     3421 gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct gttacggatt tatttaagct
     3481 ctccggtctg gtcgttggtg aactattcat tacgccatag ccagttagaa agcgtaagca
     3541 gttttattgc ctatcggcaa aagcaaatgc ataccctgaa agagattatt gccaaaccgc
     3601 gactaaccgg cagggaattt catgatgtgc gaaaaatcat cagccaacag gtttcttatt
     3661 acgacacctt aagatcgctt gatcctgaaa ataaagaggc gttacagatt tcccgtttcc
     3721 tcgccgccat taatggcctg atgggagata aacacgacga catggtggcg gatgatatgg
     3781 aaaaccgcca gtcgtatgat gcaccgttgg cgctggatag cgatattcgt cagcgcttag
     3841 agctgctgat ttcgcgtttt ccgctataac cggagcaatg atgtcatctc gtttgattat
     3901 tgcgctgatt atcatgcttt tagccccggg tgtgcaggca cacaatttcg tcactggcaa
     3961 aacggttacc cctgtttata ttcaggaagg cggcgagctg cttctgaata gcgacgacga
     4021 aattcactac caaaagtgga acagcacaca attggcggga aaagtccgca ttatccagta
     4081 tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg ttaattaaag cggttgaagc
     4141 ggcaaacttt ccgcaggatc gttttcagcc caccaccatt gtgaacaccg atgatgccat
     4201 ctttggtact ggctattttg ttgttggcaa aatcgaaaaa aataaacgcc gctatccgtg
     4261 ggcgcaattc gttattgacg gcaatggaca gggacgcgtt gcctggcgct tgccggagca
     4321 gagttcaacc attctggtgc tgaataaagc cgggcaaatt cagtgggcaa aggacggcag
     4381 cctgacgccg gaagaggtag atcatgtcat tgcgctggcg cagaaactga tcaatgaata
     4441 aaaaattcaa aggattgagg gcgttagtga gatgaagctg gttgaacact acatcatgcg
     4501 tggtacgcgc cgtctggtgc tgattatcgt cggttttctg atctttattt tcgccagcta
     4561 ctccgcacag cgttatctga ccgaagcggc aaatgggaca ttagctcttg atgttgtgct
     4621 ggatatcgtt ttctacaaag tgctgattgc actggagatg ttgttacctg ttggactgta
     4681 tgtgtcagtt ggcgtgacgc tagggcagat gtataccgat tcggaaatta ccgctatctc
     4741 tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt ctttatctgg cgataccgct
     4801 aagtattttt gtcacccttc tgtcgatgta tggtcgtccg tgggcttatg cgcagattta
     4861 tcaactggag cagcagtcgc agtcggagct ggatgttcgc cagttgcggg caaagaaatt
     4921 taacactaac gataacggac gaatgatcct ttcgcagacg gttgatcagg ataataatcg
     4981 cctgactgac gcgctgattt atacttctac tgccaatcga acccgcattt tccgcgcccg
     5041 ttcggttgat gtggttgacc catcacccga gaaaccgacc gttatgttgc acaacggcac
     5101 tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa cagatctacc gtaatctgca
     5161 attacatctg aatccgctgg ttcaaagccc taacgtcaaa cgcaaagcga aatcggtcac
     5221 ggagctggcg cgctccgtct ttcctgccga tcatgccgaa ctgcaatggc gacaaagccg
     5281 tggtctgaca gcattgttga tggcgctgct ggccatttca ttaagtcggg taaaaccgcg
     5341 gcaagggcga ttttcaacgt tattgccact gacgttgctg tttattgcca ttttttatgg
     5401 cggcgacgtc tgccgtacgc tggtggctaa cggcgcgatt cccctcattc ctggtttgtg
     5461 gttagtgccc ggactcatgc taatgggcct gctgatgctg gtcgcacgcg acttctcttt
     5521 gctgcagaaa ttttcccgat gaatgttttc agtcgctatt taatccgtca tctctttctc
     5581 ggttttgccg ccgccgcagg gctattgctg ccgcttttta ccaccttcaa cttgattaac
     5641 gaactggatg atgtcagccc gggcggttat cgctggactc aggcggtgct ggtggtgcta
     5701 atgaccttac cgcgcacact ggtcgaactt tcgccgttta tcgcgttatt gggtggtatt
     5761 gttggactgg ggcagttatc gaaaaacagt gagcttaccg ccattcgcag cacggggttt
     5821 tctatcttcc gtattgcact ggtggcgctg gttgccggga tattgtggac tgtttcgtta
     5881 ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac aggcgttgca aatcaaatcg
     5941 accgccaccg cgttggggga ggacgatgac attaccggca atatgctgtg ggccaggcgt
     6001 ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg gccagccggt gggcgtggag
     6061 atatttcatt atcgcgacga tctctcgctt gaatcctaca tttatgcacg cagtgccacc
     6121 attaaagacg acaaaacgtg ggtcctgcat ggtgtgaatc ataaaaaatg gctgaatggc
     6181 aaagaaacgc tggaaacatt agataatctt gcctggcaat cggccttcac cagtatgaat
     6241 cttgaagagt tatcaatgcc ggggaatact ttttctgtcc gtcagcttaa tcattacatc
     6301 cattatttgc aggaaaccgg gcaacccagc agcgaatacc gccttgcact gtgggaaaaa
     6361 ctggggcaac ccatcctgac cctggcgatg attttgctgg ctgtgccgtt tacctttact
     6421 gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag gcgtcatcgt gggcttactc
     6481 acctggatca gctatcaaat catggttaat ctgggattgc tatttgcgtt aagcgcacct
     6541 gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg cgttgagcct ggtgtattgg
     6601 tatgacagac aacattaaac ccgccgtggc gcaaacacca cggcgggctc acattactgc
     6661 ttcagagtag caaatgcctg aatgatggca tcgatttgct cacgactgtg ggcagcattt
     6721 acgctacagc gcagcagcgt gatgcctgca ggcgcggcag gcggcaggat aagattcaca
     6781 tacacgccca gcgagataag ctcgcgccag atgcgtaaac cttcctcttt cgaaccgatt
     6841 attactggca ctaccgggct aatatgtgaa cttaactcat aacccagttt ttgcagaccg
     6901 tcatacagat ggttggcgtt atccatcagc ttttgccgca gctctggttg tgtggcaata
     6961 gttcttaacg acgagcgcac ggtcgcaatg caggacggag aaggagaggc ggtaaaaata
     7021 tacgggcggc tgccgtagcg cagcacttcc atcgcttccg aaccgacagc aaaaccgcca
     7081 atcgaggcca gacttttact gaaagtacca acgataatat ccacatcatc ttcaacgccg
     7141 acagcttcag ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg cgcttcatcg
     7201 acaatcaaat aaccaccgag acgacgtttg atatcaacaa tttccgccag cggcgcaacg
     7261 tcaccgagca tgctgtagat gccctcaacg atgataatcg cctctttcgc ccgttcaccg
     7321 aggcgaacca tgcgccgttc cagatcttta gcatcgttat ggcggaaacg aataatttcc
     7381 gcgccaccga gagaacaggc gtcataaata ctggcgtggc tgtcggcatc cagtaacacc
     7441 acggcgttat ggtcggcaag tgcgctgatg acgcctaaat tagcggtata accggttgaa
     7501 aaaacgatag cggtagggcg attgaaaaac tcggcaatct ctttctctaa cgccaggtgc
     7561 ggcgcataac tgccgtttgc catacgagaa ccggtggtgc cggtgccttg ggccgctaac
     7621 gctgcctgac cttcgctgat ggcatcatga ttaaaggtta aaccgagata attattggtc
     7681 ccggccagaa tcactttttt attgccgatc cggccttcgg tggcggaata cacttcatca
     7741 atacaggtgc caaacggtgt taacccgttg tcactaaatt gcagtcgctc gccagcaagg
     7801 cgcgcatatt tatcgtatag ccccattatt tattctccag ccatggaagt agagtttcca
     7861 ttaattgcgc aggggttttg acatccagca gaatattaat aggaatggaa atatcgaatc
     7921 tgtcttccag catcattaag agatccatca ctttgatcga ctccaggcca agatcgttga
     7981 cgagatcgct gtcgggctta atttccacac cgttttcgac caaatcctgt aaacaggaaa
     8041 ggatgtaatc cattactatt tcacgattta ccatagaaac gttaacacac ctttaaacaa
     8101 gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc taatcccggg aaaccaatta
     8161 atatcttcag aaatacggtt attacttgcc gaccagtcgg cgtgggttaa ttcacgaatt
     8221 ttcgagcggg tcagcatagg ttctttacct gccaggcgac tcaacgcggt actgatatcc
     8281 gcaaggcagg tgagtaccgg cagaggaatg ccaaccattc gcacggaacc acaacgggcg
     8341 tcggcggcaa gctgtcgtac gtgttgccag tcatagccgc cggggacgcc atcgcataat
     8401 tcataggttt gcgtctgtac agtttcggcg cttaaccact gacccactgc ttgcgcgaaa
     8461 tcggtgacgt gcaggaaaga gagctgtgta tctggcgcac caagtcgtgg cagcaggccg
     8521 cgcagcatcc agtcaaacag cggttttaac tctttatcgc cgggaccata cacggctgtc
     8581 gggcgaaaaa cgcccagcgt aatttcgtca gccattgcag tcagacgttg ttcggcgacg
     8641 tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg ccgccagcga agagataaac
     8701 agaaaacgtt ggcaaaagcc gctctctttt gctgcttgca tcaggcgcag gctgccgtca
     8761 acgttacagt gggtaaaaat ctcttctttg tgcccgcgca cttgcccggc gcaatggacc
     8821 accgcgctgg ctccggcaac cagcttgcta agtgaatgag tatcttccag cgaaccgcgc
     8881 acccaggtga gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc gcgaacgtga
     8941 aagccgcggg cgagcaggtt attgataata tatttaccga taaacccggt agcgcccgtc
     9001 accgcgacag tttgattcat gccagggatt cctgcacatg aagactggca gcataagcct
     9061 tctgataacg ttttttcgct tccgcacggg caggcttgcc ggaggacgtt cggggaatac
     9121 tgtggggcgg caacagctcg atagccgcgg tcacgccaaa ttcgctttgg atccgtgccg
     9181 ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct gatccgacac tggatctgca
     9241 aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc gccagaatga atttccggtt
     9301 cttgttccgc aatatattca atatcctgcg gccagatatt acggccacga ataataatca
     9361 gatctttaat gcgtccggtg acatacagat aaccgtccag cagataaccg aggtcgccgg
     9421 tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaagc ctggtcgcca aagtaaccgc
     9481 tcatcagact gggaccggag atgcaaatat ggcctaccac acgttccgcg accggcatac
     9541 ctgcttcatt gcggatttca ataccatgtt ccggcaacgc tttgccgcag ttgacgaaag
     9601 tcgatacggc gcgtgtctct gcacccggcg cgacggcttt accctgatat tcgaggatgt
     9661 cgcgatccac ttcgttaacc acaaccccgg aggcttcatc agagaagctg acagccagcg
     9721 cattttctgc cagtccgtag cacggcatga aagttttatt gtcaaagtta acctgacgga
     9781 aacattcagc gaattgatgg agttgttctg cggagatggg ttctgcacca ataccagcga
     9841 cgcgccagca ggaaagatcc agttcagcga gatctttttc attcacgcgg cgctggcaca
     9901 attcatagcc aaacggcggc gcaacggaaa cggtgccgcg atttttactg atcaatttaa
     9961 gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa ataatctact gaaagctgcg
    10021 tggcgacggg ggtcaggaga aagccgacca gtcccatatc atggtagaaa ggcagccagg
    10081 agacgcagcg gtcgccaggg cgtaatttaa tgccgtcgtg gcttatagca cgtagattag
    10141 ccatcacttc gcgatgggtg ataatgacgc cacggggaaa acgggtgctg ccggaggtgt
    10201 actggaggta ggcgatatcg ttcggaactg gacgctggag cgcaacatcg gcttccggta
    10261 atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg gttgtcatgc gtcgcggcat
    10321 tgaccagtgg caaccactca tcaccagtga taatggctgc gggctggcaa cttgccagta
    10381 aaccctgcaa tttggcgctc caggaatccc gctgaccaac gcccattgga atcgccaacg
    10441 ggacggcgac taagccggca tactggcagg caaaaaaagc ctctacgaac ccgctacttg
    10501 tttcggcaat cagtgccacg cgatcgcctt ttttcaggtt cagcgataac aaccgcttcg
    10561 caccagcttc ggcacgcgtt tttaatgtct gatattccag ttgatcttca agttggcaac
    10621 gtctgtcata aaaattcatt ccggcgctac tcagagcggc gtagtccaaa gcatcaacca
    10681 gcgttggaaa atcggcatag cgcataggta gggaatgcgt aaagatttta ttagacatat
    10741 acaccacgaa aaaattggat atcaaa
//
LOCUS       DAPHJE010000138        10726 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPHJE010000138 DAPHJE010000000
VERSION     DAPHJE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.139"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(169..1851)
                     /gene="glcA"
                     /locus_tag="RH222_003814"
     CDS             complement(169..1851)
                     /gene="glcA"
                     /locus_tag="RH222_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV3798763.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2206..4377)
                     /gene="glcB"
                     /locus_tag="RH222_003815"
     CDS             complement(2206..4377)
                     /gene="glcB"
                     /locus_tag="RH222_003815"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV3798764.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQLERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGAQVIAWVRRFLDESLPLEKGSYQDVVAFKVVDKQLRIQLKNVKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMFSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSAQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4399..4803)
                     /locus_tag="RH222_003816"
     CDS             complement(4399..4803)
                     /locus_tag="RH222_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV3798765.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4808..6031)
                     /gene="glcF"
                     /locus_tag="RH222_003817"
     CDS             complement(4808..6031)
                     /gene="glcF"
                     /locus_tag="RH222_003817"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV3798766.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQLVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6042..7094)
                     /gene="glcE"
                     /locus_tag="RH222_003818"
     CDS             complement(6042..7094)
                     /gene="glcE"
                     /locus_tag="RH222_003818"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV3798767.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7094..8593)
                     /gene="glcD"
                     /locus_tag="RH222_003819"
     CDS             complement(7094..8593)
                     /gene="glcD"
                     /locus_tag="RH222_003819"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV3798768.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8839..9603
                     /gene="glcC"
                     /locus_tag="RH222_003820"
     CDS             8839..9603
                     /gene="glcC"
                     /locus_tag="RH222_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV3798769.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9610..10725)
                     /locus_tag="RH222_003821"
     CDS             complement(9610..10725)
                     /locus_tag="RH222_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3798770.1"
                     /translation="MEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSAKNP
                     GTDHIIWQAWVAKKAGQIVGRITAQIDTLHCERYGEDTGHFGMIDAIDDPQVFAALFG
                     AAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYH
                     KGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGWQ
                     HNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNGS
                     LFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRKI
                     DALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2506 a         3021 c         2706 g         2493 t
ORIGIN      
        1 cgtgataaaa aaaatagatg ttctcacgct cttaattatt tagcaggtta tctgaacaca
       61 aaacattatt gccgcatgga taataaagcg agaaataatt ttcaattaat aagacagaat
      121 aatgtaaatg aagccggatg atattaacga tcatccggca ttattgattt acgagaccaa
      181 cattccagta aacacatacg cctgcaacaa cgtgataacg ccgataacgc tggcaaaaat
      241 cagactgtgc ttcacggtgt agcggaatag ttcagattct cggcccacca tgcccgtcgc
      301 ggcgcaggcc acggcgatag attgcgggga gatcatcttg ccagttacgc cgccgctggt
      361 atttgctgcc accagcagag tgtcagagac gttgatttgc tgcgccgtgg tcgattgcag
      421 tgaaccaaac agggcgttag aggaggtgtc cgagccggta aggaatacgc ccagccagcc
      481 gagaaacggt gagaagaacg ggaacatcac gcctgtacct gccagtacca gcgccagcgt
      541 ggtggacatg ccagaatagt tggtgacgaa ggcgaacgcc agcaccatgc caatcgacag
      601 tatcggccac ttcaagctaa ttagcgtttc ggcaaagacg ccaatacctt tcttgatccc
      661 aacaccgagg atgaagatag agataatcgc cgcaataaaa atagcggtgc cgccagccga
      721 gagagggtcg aatttaaaca ccgcatccat tggcgttggt tgggcgacaa tgggtgccgc
      781 tttcaacact tgttgatgca aatgagggat ctggaaatta atcaccagtg aataaaacgc
      841 gccgcccgga gcaaataacg ctttaaacgg cttcatggtc cagatggtga ccagcaccgt
      901 taagattaaa aacggtgacc acgcacgaat gatttgcccc agactatatt ctgaaggcac
      961 gggaccgcca gaagatggct tatttaccac catcgcacct gcggattgcc ccatgctgat
     1021 tgccgtttcg gtatttttcg gccgccagac tttaaggaat aaagcgagtg agacgatact
     1081 caccagcgcc gaagtaatat ccggcagttc cggaccaata tagttagagg taaagaactg
     1141 agtgacagca aagcttcccc cagcaaccag cgccgctggc cacgtctctt tcaccccttt
     1201 ccagccgtcc atcattgcta ccagccagaa cggcacaaga accgacagga acggtaactg
     1261 gcgtcccgcc attgcgccaa tgtggaacgg atcgattccc gttacctgac cggcgaccag
     1321 aatcggcacg cccaacgcac caaacgccac tggcgcagtg ttggcaatca gacacaaccc
     1381 cgccgcgtat aacggtttga agcccaggcc caccagcagc gcaccggtaa tcgccaccgg
     1441 agcaccaaag ccagccgctc cttccagcaa cgcaccaaag gagaaaccaa tcagtaacac
     1501 ctgcaaacgc tgatcgtcgg tgatggagat aaccgagctg cggataatat cgaactgccc
     1561 gctggcaacg gttaatttat acaggaacac cgccgcgaca ataatccacg caattggcca
     1621 taatccataa ataaagccat agcccgcagc agcaaatgcc atatcaatcg gcattttaaa
     1681 ggcgaatatt gcaatcagga tagataatat aagggttatt gctccagcga catgtccttt
     1741 cagacgtaat accgcgagtg caacgaagaa gaatattatc gggatcaggg cgaccagagc
     1801 ggatagcccc agtcctccca tcggcatata catttgggtc caggtaacca tattgttcag
     1861 tctcttatta tctttatatg cttgatatac ttaaggttgt aataagcaaa agaggactga
     1921 actgtaaaat ataggcgtta tactttacag caacagtacg ccgctaacgc aattgctacc
     1981 tctggcataa caagtatatc gggtaagggt ttctgttctg cacacgcagg cgcagagtat
     2041 cgttaagatg tccatattgt tgttttaatc ccgctagtga tgcgctacgg gtatttaata
     2101 ttgttaaacc ctgataatcg ctccggttat ttccgggata aatgtactac cgcagttact
     2161 atcatagccc cgacaataaa acttgccggg gcttttttga cgctattaat gactttcttt
     2221 ttcgcgtaaa cgccaggcgt gtaataacgg ttcggtataa ccgtttggct gtttcacgcc
     2281 gaggaagatt aaatcgctgg cagctttaaa agcacacgag ttagcgaaat tccccgccat
     2341 cggacgatac gccggatcgc cagcgttttg ctgatccacc actttcgcca tattctccag
     2401 cgacgcctgc acctgttctt tggtcagaat accgtgacgt aaccagttgg cgatatgctg
     2461 gctggagata cgcagcgttg cgcggtcttc catcagcgcc acgttgtgaa tatccggcac
     2521 ttttgaacaa ccaatcccct gctccaccca gcgcaccaca taccccagaa tcccctgcac
     2581 gttgttatcc agctcttgtt ggatctcttc caccgaccag ttagcgtttt cagcaaccgg
     2641 aatagtcagc agatcgtcca gcagcggttc aaattcagca ttgaactcgg tctgggcaat
     2701 gttagcttgc gcgctctgta cgttggtttg gtggtagtgc agcgcatgga gcgtagcggc
     2761 agttggcgac ggaacccagg ccgtgtttgc cccggcacgc agctggtcgc ctttctggct
     2821 gaacatgtct gccatcaggt ccggcattgc ccacatgcct ttgccgattt gcgctttacc
     2881 gcgcagccca cagaacagac cggaaagcac gttattacgt tcgtaggctt tgatccacgg
     2941 cgtcgatttc atctggtttt tacgcagcat tgggccagct tccatcaccg aatgcatctc
     3001 atcgccggta cggtcgagga aaccggtatt gatgaacgcc acgcggttgc gcgcctgagc
     3061 aatacagcta cgcaagttca gcgaggttcg gcgttcttca tccataatgc ccattttcag
     3121 ggtattcggt gccataccga gcattgtctc aacgcgggta aacagtttgt tggcaaacgc
     3181 cacttcctgc ggaccgtgca ttttcggttt cacaatatag acgctgccag tgcgcgagtt
     3241 tttctgcact tttaaatcat aaagggcaat cgcgcctgtc atgacgccat caagaatgcc
     3301 ttccgggatt tcattgcctt cgctgtccca aatcacagga atggtcatca aatgccccac
     3361 gttgcggata aacagcaacg agcgtccgtg cagagaaatt tcagagccat cggcggcggt
     3421 gtaatgacga tcgtcattca gtttacgcac gatttgccga ccgttttttt ccattttctc
     3481 ttgcagagtc ccctgcatca ggcccagcag attgcggtac agcaggattt tatcttccgc
     3541 atcaaccgcc gcgaccgaat cttcgcagtc gagaatggta ctgatggcag cttcgacgat
     3601 aacatcgttg atgtgcgccg gatcgtcttt gccaatccgc ccgttggcat cgatttgcag
     3661 ctcaatatgc aggccgttat ttttcagcaa aatgcaggtc ggcgcagcgg tatcgccacg
     3721 ataaccgaca aactgtgccg gggtacgtaa cgtggtttct ttaacatttt tcaactggat
     3781 gcgtaattgt ttgtcaacca ccttaaacgc caccacatcc tgatagctgc ctttttccag
     3841 cggtagtgat tcatcgagga aacgccgaac ccaggcgata acctgcgcac cgcgctgcgg
     3901 atcgtagccg ctgaccatcg ccccttcctg cgggatgatg tcgctgccgt ataacgcatc
     3961 gtacagtgag ccccagcgcg cgttcgccgc gttcagcgcg tagcgggcat tcatcgccgg
     4021 aaccaccagt tgtggccccg cctggctggt gatttcgctg tcaatgcccg tggtttccac
     4081 cgtcacgcgc tccagttgcg gcaccaggta gcccagttca cgcaggaaag atttataggc
     4141 cgctttatct tttaccggcc ccggattgct gcgatgccac tcatcaagcg ccgcctgaat
     4201 gcgatcgcgt tctgccagca actgacgatt ttctggtgcc aaatcgtgaa cgatttcatc
     4261 aaaattgcgc cagaacgccg cagcgtccag ccctgttccc ggtaaaactt cttcatccac
     4321 aaaacgttta aaattggcgt caatgcgtaa acggccctgg gttatggttt gactcattgt
     4381 ttatctcctc gttttcgctt atttcgccaa caccgctgct gccgctttcg cgacctgcgc
     4441 atcctgtgct ccggttaaac cagaaacgcc cacggcacca ataatttgcc catccacaac
     4501 aactggtacg ccgccttcca gcgacgtcag taacggcgca gtcacgaacg cggtacgtcc
     4561 gttgttcacc atctcttcat agcccttagt ttcacgacgc cccagcgcgg cggtacgcgc
     4621 tttctcctgg gagatgtaag ccgcaatcgg cgcgcaatcg tccatgcgac ttaacgccag
     4681 cagatgaccg ccgtcatcgg caacagcaat ggaaaccgac cagttatttt tctgcgcctc
     4741 ttcctgacct gcggcaataa ttgcactcgc catttgctgg ctaagaatga ctttagtttt
     4801 cattttgtta ttccttttca agggcttgtt ctacaatttc aatccagtga cgcacagagg
     4861 tacgaccggc gctcgccaga tgcgtctggc aaccaatgtt ggcggtgacg atcatttccg
     4921 gtttgccgct ttccagcgca ttcattttgt tatcccgcag ctggcgtgcc agatcgggat
     4981 gcgttaacgc atatgttccc gctgaaccgc agcacagatg gctgtcggga acgtccgtta
     5041 aggtaaatcc aagacgaagt aacacttttt ccacttcgcc gttcagcttt tgcgcatgtt
     5101 gcagggtaca cgggcagtgg aaggccagtt ttttatcgcc gcgaattgcc agtttttcca
     5161 gcggttcctc gcgcagaagt tcgactaaat cgaccgccag ttcactgact tgacgcgctt
     5221 tatcggcata taacgcatcg tttttcagca tctgcccata ctctttgacg aacgcgccgc
     5281 aaccgctggc ggtttgcaaa attgcctcgg cacctgcttc aatcgcgggc caccaggcat
     5341 caatattatt tcgcgcccgt gccagccctt tctcctgcgc attaagatga tagtccaccg
     5401 cgccacaaca gcctgcttcg ttagctgaca tgacgctgat ccccagacga tccagcactc
     5461 gcgcagttgc cgcgttggtg ttgggcgaaa gcgtaggctg ggcgcagcct tccagcatta
     5521 aaacccgacg cttatggcgc agcggcggac gcggtttagc tttcacagtt tcagcaggca
     5581 gttttgctct gacctgttcc ggtaaaaacg gtcgcagcac cagccctacc tgcgtcagcg
     5641 cacggaagac cgccggacgc ggcactaact ggcgcaatcc ttcgcgcagt attcgctccg
     5701 gcagtgggcg tttcactttc tgctcgacaa tatcacgccc gatatccagc aaattgtgat
     5761 agcgcacacc agaaggacag gtggtttcac aattgcggca agtgaggcag cgatcgagat
     5821 gctcctgtgt tttaagcgtg acttcgttgc cttccagcac ctgtttaatc agatagatgc
     5881 gcccgcgcgg cccgtccagt tcatcgccca gaagctgata ggttgggcag gttgcggtac
     5941 aaaatccgca gtgaacacag gcgcgcagga tgctgtcggc ttccagcgcg cgcgcgttct
     6001 gccgcatctc ttcagttaat tgggtttgca tagcctgctc ctcagagttc cgcgtacatg
     6061 cgaccggggt taaacacgcc gcaagggtcg agctgctgtt taagctgctg gtgatagcgg
     6121 aataaaggag ccgatagcgg ggcaaagcca ccatctccgg cactaaagcg ggtcgcatga
     6181 ccgccagcgt tgcgggcgat gcgatggatt tgattgtcct cggctgtcga tttcagccag
     6241 cgtaacgcac cgccccagtc gatcagttgc tcgccgggta aatccatcat cggcgcatcg
     6301 ctgggtaatg aaatgcgcca taaggtacct ggtaacgaga agaacggcag ttgttgttca
     6361 cgcaattgct gccagaactg acccgcaacc tcttcgcccc ccagcagttc acgcgccgct
     6421 tttaccgatc cttcgccgcc ctcaaggcgg atccacaacg cattgtcgaa gtaacataag
     6481 ccactaatag gtagcggctg gagttgccac tgggcgattt cgttcatggc ttcttgcagg
     6541 ctgatttccc gacgcaggct cagggaggcg cgcggtcgcg gtaacacttt catcgagatt
     6601 tcagtgagca cgccaagaca accgtagctt ccggccatta accgtgagag atcgtatccg
     6661 gcaacgtttt tcatcacttc gccaccaaaa cgcagatgtt ttccagcgcc ggtaatgatg
     6721 cgcgtgccga ggacaaaatc gcggaccgaa ccgctccacg ggcgacgcgg ccccgccagc
     6781 ccgcaggcga ccatcccgcc ccaggtggct tcttcaccat aatgcggcgg ctcgcaggga
     6841 agcatttgcc cggcgctctc cagcgccgct tcaatcgcca ccagcggcgt tccggcacgc
     6901 gcggttatca ccagctcggt cgggtcgtaa ttaacaatgc cgcgatgaca acgcacatcc
     6961 agcgtttgcc cggtgacagg gcgacctaaa aaggctttgc tattgctgcc ctgaatcacc
     7021 agcggcgttt tatcgctaat tgcctgattc acctgctcca gcagcgcctg actgtaatca
     7081 cactcgcgta gcatcagaaa cgctccagtt cagggaaagg taaatgaccg tgatgcacat
     7141 gcatggcacc aaattcagca cagcggtgta gcgtgggaat gtttttccca gggttcagca
     7201 aaccatcggg gtcaaacgcc gccttgaccg catggaaggt cgtgatttca tcgctgttga
     7261 actgggcgca catttgattg attttttctc gcccgatgcc atgttcccca ctgatgctgc
     7321 cgccaacttc aacgcagagt tcgaggatct tcccacccag ctcttccgcg cgggcaaatt
     7381 caccgggttc gttggcatcg aaaaggatta acgggtgcat gttgccatct ccggcatgaa
     7441 agacgttggc aacacgtaaa tcatattgct gcgataaacg ggcaatgcct tccagtacgc
     7501 caggcagggc gcgacgcggg atggtgccat ccatgcagta gtaatccggg gagatacgtc
     7561 ctaccgccgg gaacgcattt ttgcgaccgg cccagaaacg tacgcgctct gcttcgtcct
     7621 gtgccagacg gacgtcagtc gcgcccgctt tcaacaagat gtcgttaacc cgctcgcagt
     7681 cttcctgtac gtcagactcc acgccgtcca gctcgcataa caaaattgct tcggcgtcga
     7741 cgggataacc ggcatgaata aaatcttccg ccgcgcggat cgacaggtta tccatcatct
     7801 ccagcccgcc ggggataatg ccattggcga tgatgtcacc aactgcaagt ccggcttttt
     7861 ctaccgagtc aaagctggct aacagaaccc gcgccacggg cggcttcggc agcagtttta
     7921 ccgtcacttc ggtggtcacg ccgagcatac cttccgatcc ggtgaacagc gccagcaggt
     7981 caaaaccagg tgaatccagc gcgtccgatc ccagcgtcag cgcctcgccg tccagcgttt
     8041 gcacttcaat tttcagcagg ttatgtacgg tcagaccata tttcaggcag tggacgccgc
     8101 cggcattttc tgccacgttg ccgccaatgg aacaggcgat ttgtgaggaa gggtccggtg
     8161 cgtagtagag attatgcggt gcaacggcct gggagatcgc caggttacgc acgcctggct
     8221 gcacgcgcgc gcggcgacca acggggttaa tgtcgaggat ctctttaaag cgcgccatca
     8281 ccaacaacac acctttttcc agcggcagcg cgccaccaga aagcccggtg cctgcaccac
     8341 gggtcaccac cggtacacgc aggcgatggc agacagccag aatcgctgtc acctgttcca
     8401 tttgcttagg cagaacaacc agtaatggac gcgtgcgata cgcgctcaac ccgtcacact
     8461 cgtaaggaat gatctcctca tcggtatgca ggatctcaag tccagggaca tgctcacgca
     8521 gtgccatcag taccgatgtg cggtcgacat cgggtaaagc gccatcaaga cgctcttcgt
     8581 acaagatgct catgagtagg cttcgctttg ttgtgtggca gctgattttt gcgcgctgct
     8641 tctgtgaaca gttattaagc gggcttttcg ttttcgtcta tctctttagc taccggtcag
     8701 accatttttt ttccagctct gtgaccttgc cttggttaac tcaatgttaa attgatgtaa
     8761 cataatcact tacgtgatgt gcgtgttttg cgagttaaga acagaaaaat tggtcctacc
     8821 tgtgcacgag gtccgggaat gaaagatgaa cgtcgcccta tttgcgaagt ggttgcagag
     8881 agtatcgaac ggttaattat cgacggcgta ctgaaggtcg gtcagccact tccctcggaa
     8941 cgtcgactgt gtgaaaagct cggcttctca cgctccgcac tgcgtgaagg gctgaccgtg
     9001 ctgcgcgggc gcgggattat tgaaacggcg cagggtcgcg attctcgtgt cgcacggctt
     9061 aatcgggtgc aggacaccag cccgctgatc catctgttca gtacgcagcc gcgaacgctg
     9121 tacgatctgc tcgacgttcg cgcattactg gagggcgaat cggcaaggct ggcggcaacg
     9181 ctgggaacgc aggctgattt tgttgtgata acccgctgtt atgaaaaaat gctcgccgcc
     9241 agtgagaaca acaaagagat ttcgctgatc gaacatgcgc agttggatca cgctttccat
     9301 ctcgccattt gtcaggcttc tcacaatcag gtgctggtgt ttacgctgca atcattgacc
     9361 gatctgatgt ttaattcagt gtttgccagc gtaaataatc tctaccatcg accacagcaa
     9421 aaaaagcaga tcgatcgcca gcatgcgagg atctacaacg cggtgttgca gcggctgccg
     9481 cacgtcgctc agcgcgcagc acgcgatcat gtgcggaccg tgaaaaagaa tctccacgat
     9541 atcgagctgg aaggccacca tttgattcgc tcggcggtgc cgctggagat gaacctgagt
     9601 tagctggtat taaatctgct tttcatacaa tcggtaacgc ttgtacggct ccgccccaat
     9661 gcgttccagc atgttattca tgcctgtatt ggtttcgagg atccatgaca tctccagcgc
     9721 atcgatcttc cggcgggcaa acggatcgcg taaggcttca atcaataaca gcgcaatcac
     9781 cgggccgatg cggctgaact gatactcgtc gcgcacgccc atcagcggta ctcgcgcagt
     9841 tcgcacaccg ctgactttca aacgccacag caattttgcc cagccgaagg gaaagagcga
     9901 tccgttcaga tcggcaatcg cctcgttgat gttcggcaag ccgacaataa acgcgcaggg
     9961 tgcagaatca atctcagcga tgtagatcat atcgtccggc accagatatt taagttgatc
    10021 gcccatggtc gcgaattcat gttcggtaaa cggcacaaat ccccagttgt gctgccagcc
    10081 agagttgaaa atctcacgca ggatctgcat ctcttcggca aaccgctgac gattgatgca
    10141 gcgaatggtc acctttttgc gcacctgatc catcagtttt tttagcgccg gagagaaagt
    10201 gagatcggtt cgctgcatcc accacgccag taaatcaatg cctttgtgat aacccagttg
    10261 ttcaatatgc gcggcatacc acggtttgcc gtgtggcatc atcgcacagg gtggtgtgtc
    10321 aaaaccttca atcagtaatc cgctttcctg attgatattc aggctgaaag gcccgctgat
    10381 cttacttgca ccttgtgact tcaaccacgc ttccgctgcg ccaaacaacg cggcaaaaac
    10441 ctgcggatca tcaatggcgt caatcatgcc gaaatgaccg gtatcttcac cgtaacgctc
    10501 acagtgcaag gtatctattt gcgcggtaat acgcccaact atctgccccg ctttttttgc
    10561 cacccacgcc tgccagatga tatggtccgt ccccggattt ttcgcagaca aatgctcgtt
    10621 gcgttcaatg aataaagggg gtatccagtt tggatcgtcg ggataaagtg atgacggaaa
    10681 agcgataaat gccttaaggt catttttatt aattactttt tcaatt
//
LOCUS       DAPHJE010000139        10595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPHJE010000139 DAPHJE010000000
VERSION     DAPHJE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.140"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            167..709
                     /locus_tag="RH222_003822"
     CDS             167..709
                     /locus_tag="RH222_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798771.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            809..2011
                     /locus_tag="RH222_003823"
     CDS             809..2011
                     /locus_tag="RH222_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV3798772.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2066..2536
                     /locus_tag="RH222_003824"
     CDS             2066..2536
                     /locus_tag="RH222_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3798773.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2577..3479)
                     /locus_tag="RH222_003825"
     CDS             complement(2577..3479)
                     /locus_tag="RH222_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV3798774.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3673..4863)
                     /gene="glpC"
                     /locus_tag="RH222_003826"
     CDS             complement(3673..4863)
                     /gene="glpC"
                     /locus_tag="RH222_003826"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV3798775.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4860..6119)
                     /gene="glpB"
                     /locus_tag="RH222_003827"
     CDS             complement(4860..6119)
                     /gene="glpB"
                     /locus_tag="RH222_003827"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV3798776.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6109..7737)
                     /gene="glpA"
                     /locus_tag="RH222_003828"
     CDS             complement(6109..7737)
                     /gene="glpA"
                     /locus_tag="RH222_003828"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV3798777.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            8010..9368
                     /gene="glpT"
                     /locus_tag="RH222_003829"
     CDS             8010..9368
                     /gene="glpT"
                     /locus_tag="RH222_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV3798778.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9373..10449
                     /gene="glpQ"
                     /locus_tag="RH222_003830"
     CDS             9373..10449
                     /gene="glpQ"
                     /locus_tag="RH222_003830"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV3798779.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         2429 a         2632 c         2916 g         2618 t
ORIGIN      
        1 gtgtatcggt aaagtgtgtt tattttttcg gcgtttaatt atttaagaag tatttagtta
       61 caataagtga ctagatccct tttttcgctc attgaatcct ctgtgccagg cagtacaatc
      121 ccgcagcttt gggaattttg attaattgct tgtgggaaag gggataatga aaaaaattgc
      181 gctagcaggt ctggccggaa tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag
      241 cggccaggcg ggtaaagaat acaccaatat tggtgtcggt tttgggactg aatcgacggg
      301 cctggcttta agcggtaact ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg
      361 gctggggttg aatctgcctc tcgggccgtt aatggcgacc gttggcggaa aaggcgttta
      421 caccaacccg aattacggcg atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg
      481 gaaaattggc aacagcttcc gtttgtttgg cgagtattac tactctccgg attcgctctc
      541 cagcggtatt caaagttatg aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc
      601 agtcagtatt gaggcgggtt atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga
      661 caacgccgtg gctgacggcc cgtatgttgg ggttaacgcc agtttctgat cccttccggc
      721 gcggcattct gtcgcgccat tcagtttctt actacgcctt atctcccttt gcgttatagt
      781 gattcgataa caaaagcagg agataaccat gttaaaagtg gaaatgttat ccaccgggga
      841 tgaagtgtta cacgggcaaa tcgttgacac taacgctgcc tggctggccg attttttctt
      901 tcatcagggg ctgccattat ctcgccgcaa tacggtgggg gataaccttg atgacttagt
      961 caccattctt cgcgaacgta gtcagcacgc cgatgtgctg atcgttaacg gtgggctggg
     1021 accgaccagc gatgatttaa gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt
     1081 gctgcatgaa gcctggctca aagagatgga acgctatttc cacgaacgtg gacgagtaat
     1141 ggcaccgagc aaccgtaaac aagcggagct gcctgccagt gctgaattta tcaataaccc
     1201 ggtaggcacc gcctgtggtt ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc
     1261 cggcgtaccg tcagaattta aggtgatggt cgagcacgaa atcctgccgc gcctgcgcga
     1321 gcgtttttct ttaccacagc cgccggtttg tctgcgttta actacctttg gtcgttcgga
     1381 aagcgatctg gcacaaagcc tggacactct acaactgccg ccgggcgtaa caatgggcta
     1441 tcgctcctca atgccgatca tcgaactgaa actcaccgga ccggcaagcg agcaacaggc
     1501 gatggaaaaa ctgtggctgg acgttaaacg tgttgccgga cagagcgtga ttttcgaagg
     1561 caccgaagga ctgcccgcgc agatcagtcg cgaattgcaa aaccgccagt tcagcctgac
     1621 gttgagcgag caattcaccg gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc
     1681 attgctggcg tgtgaagtgg ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg
     1741 gattacagaa cggcgggcca accattttgc cgggctggca ctggctgttt cgggtttcga
     1801 gaacgagcat ctcaactttg cgctagccac gccagacggc actttcgctc tgcgtgtgcg
     1861 tttcagcact acgcgctaca gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact
     1921 gaatatgctg cgccgttggt taaacggcca ggatatcgcc agtgagcatg gctggattga
     1981 ggttattgag tccatgacct tatctgtctg attccgccac tctgtgccgg tttactgcac
     2041 cggcacattc cccctccggc aaaacttgtc caaccctccc tacggtgagc gtggtgttgg
     2101 ggccagtgtg gcacgggctg cgcgctgggg acgcattgag aattacatgg cgcaagtcaa
     2161 cgattcgctt tgtctgttag tgcaggtgga aagtaaaacg gcactggata acctggacga
     2221 gatcctcgac gtcgaaggga ttgatggcgt gtttattgga ccagcggatc tttctgcgtc
     2281 gttgggctac ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat
     2341 tcggcggatc cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc
     2401 gcagcaatgc ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta
     2461 cagcgatgcc ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat
     2521 aaaaggtagt tattgatatc aaaggcccat ggggatcggc tgtgggcctg tgttaattag
     2581 tggttattcg ctgccagatc ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga
     2641 tcaatgccct gttccagcat gcgcaaagca attttaatgg cttgttcatg cataccttcc
     2701 tgccagccaa tttgatgccc ttcctgccgt agccgttcag caatagtcat taatctctcc
     2761 ttttgtagtg gtgaacgttc ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg
     2821 ccgcattgca gcagataatt aaacagtgtt tgtagctggc tgtcattagt gaagcctcta
     2881 accaaaagcg tggtgatcct gtcgaccatg ccgattaaat cgcggtcgcg aatatgcttt
     2941 tgaatcagtt ccagcagagc tatacgccga tgttgcatga tctcatcgtc aggtacgatg
     3001 gtgatatcca ccaacggaaa agcttcggtg tacaactgcc gggcaagttg cggatcgtca
     3061 aactcatcca gccagttaag tgagtacggg tagggcgatg tttcgccatg ataaaacagc
     3121 aatggcacca ccagcggaac tctgtcatag cctttatcca ggtgacgctg catggcggca
     3181 gtggcatagc gcattagccg aaaagccata ttcttttctg cagagctttg atgttcaatc
     3241 acgcagtaga tatagccgtc accgtcgctg gtttccacgg accacaaaac atccgagtag
     3301 taagcgcgta aacttttttc aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg
     3361 caaagcttgc gcagtggttc tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt
     3421 gtgaacataa aggttttaaa caccgcatca tgcggggagg atgttgttga ttcggtcatg
     3481 gttgtccgtc acctgatatt tatgatgacg cgacattacg tctatcccgg gcctttgaca
     3541 tctgtcagtt tcagcctttg cgagacattt tcagaggaat tttcaaagcg ttgcaacgca
     3601 tacatggtgc gtggaatcgc gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca
     3661 tcaaaaggtc ggttaagcca gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag
     3721 gctcgtggac atctcaatct gccatttgca ggtttcgcag tcggtgatga ccagatccgc
     3781 gccgctctct tctatctggc ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt
     3841 ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca gcaccgttaa
     3901 ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc ccattttttc
     3961 catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt
     4021 gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat cgcgcaagcc
     4081 tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg tacaggttga
     4141 ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg actctacatt
     4201 cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg gtacgccgca
     4261 gcatttttct ttgctgagca gttgtacacc ggtacccatt gcgttgagca ctttaattaa
     4321 atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg
     4381 gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgaa acgtaccgaa
     4441 ggagtatttc ggtagcgtgc ggcgatgatc gatttttaac gccgcatcaa gcagctgccg
     4501 caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg aaacgctacc
     4561 catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt
     4621 tgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg cgacttcaca
     4681 acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg
     4741 cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat tcacccggct
     4801 caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt
     4861 cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc actgacggca
     4921 caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg
     4981 gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt
     5041 acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc
     5101 gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt acgttccgct
     5161 accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga
     5221 atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca ggtcactttt
     5281 ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg ctgtaactgg
     5341 ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa
     5401 ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc
     5461 atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa cagcggccag
     5521 ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt ggcgttattg
     5581 cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag atccagttcg
     5641 cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat ccccactaca
     5701 catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa ccaggttgca
     5761 cgcaaagtgc cgagcggggt aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc
     5821 gcaccgcttt cagcgatcaa tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggt
     5881 tcgagaagac tgtaaggatg ggctggtgcc tgctgacgca aagattccag tccactgtga
     5941 atgtctgtca ccggttgacc atcaggcaga tggctcagca aatccagcga accggatgag
     6001 aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg tttttgcagt
     6061 tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc
     6121 tttctgctcc ttctccagac cacacaatcc ctgataaacc cagcgggtaa attcgctttc
     6181 gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt taaggaaggt
     6241 ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc
     6301 agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg tacgacggcg
     6361 taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgaactt cacccgcagt
     6421 taccgcttcg cactcacata ccaggctacg atgcagacgg ccttcgctca gccaggcagg
     6481 cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca gggagatgac
     6541 tttacgcaag gtaacttcag cgggttcttg tgaaccaggc agtgccagat cggcagtcgt
     6601 acaggggcgc gtgttgccca gtttgcggca taccgcgtcg gtagcccatt cagccatcag
     6661 ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac catcgcgttc
     6721 agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt cgtcatcgct
     6781 ggcaaccagt gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg
     6841 ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca ctcgattatc
     6901 gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc
     6961 aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat gctggttaat
     7021 gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc gcagatcggc
     7081 atattcggca atgtgttgcc cccagatccc agcggcatta accacgacag gtgcatgaag
     7141 ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgcacaccg tcgcgccttc
     7201 acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt ctttggcatc
     7261 cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc
     7321 gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg ggtctatagc
     7381 ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc
     7441 atcttccggc agggtgataa acaggccgtt ggttggttca acgcagtgac gtgcaatgcg
     7501 tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg ttaccgcata
     7561 gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg
     7621 gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc
     7681 cgttgcgccg ccaccaatga taatcacgtc acttgattgc gagtcgcgag ttttcattgt
     7741 tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa ttgtttgatt
     7801 tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat
     7861 tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttctg aatgttttct
     7921 taacatcgcg gcaactaaag aaacggcagg ttctcccact gaatcagtct gttaatcata
     7981 aataagacca cgggccacgg aggctttcaa tgttgagtat ttttaaacca gcgccacaca
     8041 aagcgcgctt acctgctgcg gagatcgatc cgacttatcg tcgattgcgc tggcaaattt
     8101 tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac tttgcgcttg
     8161 ctatgcctta tctggtcgag cagggattct ctcgcggtga tttaggtttc gcgctttcgg
     8221 ggatctcgat cgcttatgga ttttcgaaat tcataatggg ttcggtatca gatcgctcga
     8281 atccgcgcgt tttcctcccc gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg
     8341 gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtacttttg ttcctctgcg
     8401 gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc
     8461 agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtt ggtggtggta
     8521 ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcac gcggcgctct
     8581 atatgcccgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg atgcgcgata
     8641 ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat ccggacgact
     8701 ataacgaaaa agcggaacag gagctgacgg cgaagcaaat cttcatgcag tacgtactgc
     8761 cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg
     8821 gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg ctggataaat
     8881 cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcactctg ctgtgcggct
     8941 ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc tttatgacac
     9001 tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca accgtcgata
     9061 tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg atcggtctgc
     9121 atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt accgggctgt
     9181 ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc gtggacttct
     9241 tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg gttatcttgt
     9301 tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg
     9361 gaggctaatg gcatgaaatt gacgctgaaa aaccttagca tggcgatcat gatgagcact
     9421 atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt catcgcccat
     9481 cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat ggcgtatgcg
     9541 cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca tctggttgtt
     9601 ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc ggatcgggcg
     9661 cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa
     9721 tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc
     9781 ccaatgggta agtccgactt ccgggtgcac acctttgaag aagagattga atttgttcag
     9841 gggttaaatc actctaccgg gaaaaatatc ggtatttatc cagaaatcaa agcgccgtgg
     9901 ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat
     9961 ggttacaccg gtaaagacga taaagtttat ttgcaatgtt ttgatgctga tgagctgaag
    10021 cgtattaaga atgagctgga acccaaaatg ggcatggagc tcaatctggt acagctgatt
    10081 gcctataccg actggaatga aacgcagcag aaacagccgg atggaagctg ggttaattac
    10141 aactacgact ggatgtttaa gccgggtgcc atgaaacagg tggcggaata tgcagatggt
    10201 attggtccgg attaccatat gttgattgag gaaacatcgc agccgggtaa tatcaaactc
    10261 actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta taccgtgcgg
    10321 tcggataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc tctgtataac
    10381 aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa attccttaat
    10441 aaagagtaat gctattgcag gatggcgttc atgcgccatc ctctgctaaa gcgaaataat
    10501 gctactaccg ttgttctatt cattaaccgg tgcatcctgt taaaaaaatg ccaacatatt
    10561 tgcgtaaaac tcattcgtta gggaagctaa cactg
//
LOCUS       DAPHJE010000140        10509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPHJE010000140 DAPHJE010000000
VERSION     DAPHJE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.141"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            101..676
                     /gene="ssuE"
                     /locus_tag="RH222_003831"
     CDS             101..676
                     /gene="ssuE"
                     /locus_tag="RH222_003831"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV3798780.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            669..1628
                     /gene="ssuA"
                     /locus_tag="RH222_003832"
     CDS             669..1628
                     /gene="ssuA"
                     /locus_tag="RH222_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV3798781.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VTAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1625..2770
                     /gene="ssuD"
                     /locus_tag="RH222_003833"
     CDS             1625..2770
                     /gene="ssuD"
                     /locus_tag="RH222_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV3798782.1"
                     /translation="MSLNMFWFLPPHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2781..3572
                     /gene="ssuC"
                     /locus_tag="RH222_003834"
     CDS             2781..3572
                     /gene="ssuC"
                     /locus_tag="RH222_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV3798783.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3569..4336
                     /gene="ssuB"
                     /locus_tag="RH222_003835"
     CDS             3569..4336
                     /gene="ssuB"
                     /locus_tag="RH222_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3798784.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4543..7155)
                     /gene="pepN"
                     /locus_tag="RH222_003836"
     CDS             complement(4543..7155)
                     /gene="pepN"
                     /locus_tag="RH222_003836"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV3798785.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQCTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7421..8623
                     /gene="pncB"
                     /locus_tag="RH222_003837"
     CDS             7421..8623
                     /gene="pncB"
                     /locus_tag="RH222_003837"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV3798786.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVNPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8792..10192
                     /gene="asnS"
                     /locus_tag="RH222_003838"
     CDS             8792..10192
                     /gene="asnS"
                     /locus_tag="RH222_003838"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV3798787.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2377 a         2711 c         2820 g         2601 t
ORIGIN      
        1 acacaaaatt tatatttggc attttcttgt ctctccgaca gactgaccga attgttatca
       61 atgttaacaa aaaaagaaca attggttata aggagagagt atgcgtgtca tcaccctggc
      121 gggtagtcct cgctttcctt ctcgctccag ctccttgctg gaatatgcgc gggaaaaact
      181 aaattgcctg gatgtagagg tttatcactg gaatctgcaa aacttcgccc cggaagatct
      241 gctttacgct cgtttcgata gtccggcact caagaccttc accgaacagc tgcaacaggc
      301 tgatggactg atagtcgcta cacctgtgta taaagccgcc tattccggcg cgctgaaaac
      361 cctgctcgac ctgctgccag agcgcgcttt gcaaggcaaa gtggtgctac cgctggcgac
      421 gggcggtacg gtggctcatc tgctggcggt cgattatgcc cttaagccag ttttaagtgc
      481 gatgaaagcc caggagatcc tgcacggcgt gtttgccgat gactcacaag ttattgatta
      541 ccatcacaaa ccccagttca cgccaaatct gcaaacccgt cttgataccg cgctagaaac
      601 tttctggcag gcattgcacc gccgcgatgt tcaggttcct gaccttctgt ctctgcgagg
      661 taatgtccat gcgtaacatc attaaactgg cgctggcggg attgcttagc gtctctactt
      721 ttgcggttgc tgcagaatcc tcgcctgaag cgttacgtat aggctatcag aaaggcagta
      781 ttggtatggt attggcaaaa agccaccagt tactggaaaa acgctatccg caatcaaaaa
      841 tctcctgggt ggagttcccc gcgggtccgc aaatgttgga agcgttaaac gttggcagta
      901 ttgatctcgg cagtaccggg gatattccgc caatctttgc ccaggctgcc ggggctgatt
      961 tggtatatgt gggcgtcgag ccaccgaagc ccaaagccga agtgattctg gtggcagaaa
     1021 acagcccgat caaaaccgta gccgatctta aaggtcacaa agttgccttt cagaaaggtt
     1081 ccagttcaca caacctttta ctgcgtgcac tgcgtcaggc cggacttaag tttaccgata
     1141 tccaacccac ttacctgacg cccgctgatg cccgcgccgc gttccagcaa ggtaacgttg
     1201 acgcctgggc tatctgggat ccctactact ctgctgcatt attacagggc ggcgtgcggg
     1261 tgctgaaaga cggcaccgat ctcaatcaaa ctggatcgtt ttatctggca gctcgtccct
     1321 atgcagaaaa aaacggcgct tttattcagg gcgtactcgc aacctttagt gaggccgatg
     1381 cgttaacccg cagccagcgc gagcaaagca tcgctttact ggcaaaaacg atgggcttac
     1441 cggcaccggt gattgcctct tacttagatc atcgccctcc taccaccatc aaaccggtta
     1501 ccgccgaggt tgccgcctta cagcagcaaa cggcagatct gttttatgaa aatcgtctgg
     1561 tgccgaaaaa agtcgatatt cgccagcgca tctggcagcc cactcaactg gaaggaaaac
     1621 aattatgagt ctgaatatgt tctggttttt accgccccat ggtgacgggc attatcttgg
     1681 aacggaagaa ggttcacgcc cggttgatca cggctatctg caacaaattg cgcaagcggc
     1741 ggatcgtctg ggctataccg gtgtgctgat cccaacgggg cgatcgtgtg aagatgcgtg
     1801 gctggtggcg gcgtcgatga tcccggtgac gcagcggctg aagtttcttg tcgccctgcg
     1861 ccccagcgta acctcaccta ccgttgccgc tcgccaggcc gccacgcttg accgtctctc
     1921 aaatggtcgt gcgttgttta atctggtcac aggcagcgat ccacaagagc tggcaggcga
     1981 cggcgtgttc cttgatcata gcgagcgcta cgaagcctcg gcggaattta cccaggtctg
     2041 gcggcgctta ttgctggggg aaaccgtcaa tttcaacggt aaacatattc atgtgcgcgg
     2101 agcaaaactg ctcttcccgc cgattcaaca gccttatcct ccgctttact ttggcggatc
     2161 gtcagacgtc gcccaggagc tggcggcaga acaagttgat ctctacctca cctggggcga
     2221 accgccagaa ctagtaaaag agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg
     2281 acgcaaaatt cgtttcggta ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc
     2341 gtggcaggcc gccgagcggt taatctcgca tcttgatgat gaaactatcg ccaaagcaca
     2401 ggccgcattc gcccggacgg attccgtagg acaacagcga atggcggcgt tacataacgg
     2461 caagcgcgac aatctggaga tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg
     2521 cggtgccgga acggcgctgg tgggcgatgg tcctacggtc gctgcgcgaa tcaacgaata
     2581 cgccgcgctt ggcatcgaca gctttgtgct ttcgggctat ccgcatctgg aagaagcgta
     2641 tcgggtcggc gagttgctgt tcccacatct ggatgtcgcc attccggaaa ttccccagcc
     2701 gcagccgctg aatccgcaag gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc
     2761 gcaaagctaa ggaagaaata atggcaacgc cagtgaagaa gtggttattg cgcgttgccc
     2821 cctggttttt accggtgggc atcgtggcgg tgtggcaact ggcctcctca gttggctggc
     2881 tgtcgacgcg tattttgccc tcaccggaag gggtggtgac ggcgttctgg acgctctccg
     2941 ccagcggcga actgtggcag catctggcga tcagttcctg gcgggcgctg attggctttt
     3001 caattggcgg atcgctgggg ttgattttgg gactgattag cgggctatct cgctggggag
     3061 aacggctgct ggatacctca attcagatgt tgcgcaacgt accgcatctg gcgctgatcc
     3121 cgctggtgat tttgtggttt ggcattgatg aatccgcaaa aatctttctg gtggcgctcg
     3181 gcacgctatt ccccatttat atcaacacct ggcatggcat ccgtaatatc gatcgcggtc
     3241 tggtggaaat ggcgcgtagc tatggattat ccggcatacc gctgtttatc catgtgatcc
     3301 tgcctggtgc cctgccctca attatggtcg gcgtgcgttt tgcgttgggc ctgatgtggc
     3361 tgacgctgat tgttgccgaa accatttctg ccaattcagg cattggttat ctggcgatga
     3421 atgcgcggga gtttttgcaa acggacgtgg tggtagtcgc tattattctt tacgccctgc
     3481 tcggcaaact tgctgacgtc agcgcgcagc tgttagagcg cctctggttg cgctggaacc
     3541 cggcttatca tttgaaggag gccacggtat gaatactgct cgtctgaacc agggcacacc
     3601 attgttgctc aatgcagtaa gcaaacatta cgcggaaaat atcgtcctga accaactgga
     3661 tttacatatt ccggcaggtc agtttgtggc ggtggtgggc cgcagcggtg gtggcaaaag
     3721 taccctgctg cgcctgctgg caggtctgga aacgccaacc gcaggcgatg tgttagcggg
     3781 caccacaccg ctggctgaga ttcaggaaga cacgcgaatg atgtttcagg atgcgcgtct
     3841 gctgccgtgg aaatcggtga ttgataacgt tgggttaggc cttaaaggcc agtggcgcga
     3901 tgccgcgcgt cgggcgttgg ctgcagtagg actggagaat cgcgccgggg aatggcctgc
     3961 cgcactttct ggcgggcaga aacagcgtgt ggcgctggca agagcgttga ttcatcgacc
     4021 gggtttattg ttgcttgatg aaccgctcgg ggcgctggac gccttaacgc gactcgagat
     4081 gcaggatttg attgtgtcac tttggcagca gcacggcttt accgtactgt tggtgacgca
     4141 tgatgtgagc gaagcggtag caatggctga ccgggtgttg ttaattgaag agggaaaaat
     4201 tggtctggat ttgacagtgg atatcccccg tccgcgtcga ttagggtcgg tgcgattagc
     4261 ggagctggaa gcggaagtat tgcaacgggt aatgcggcgt gggcattctg aacaactgat
     4321 tcggcgtcat ggttaatatg ccggatgtgg cgcacaaaac cagcattttc aacggactag
     4381 taggcctgat aagacacgtc aagcatcgca tcaggcagtt gggcacggct gccggatgcg
     4441 gcgtgaacgc cttatccagc ctacaaaacc agcaatttca acggagttgt aggcctgata
     4501 agacgcgtca agcgtcgcat caggcatttt ggctcaaaag tatcaagcca gtgctttggt
     4561 gatcttctcg tacagatcgc cagagagatt ttccagccct ttcaactgtt ccagcgccgc
     4621 gcgcattttc tcctgacgtt tggcatcata acgtttcagg cgaatcagcg gttcaatcag
     4681 acgtgaagct acctgcgggt tacggctgtt gaggtcggta agcatttcca ccaggaactg
     4741 gtaaccgctg ccatcttcgg catggaacgc tgccgggttg ctgcccgcaa acgcaccaat
     4801 caacgaacga atacggttcg ggttgctcat ggtaaatgag cgatgctgca acagaccacg
     4861 caccgtctcc agcacattcg ccgccgggct ggtggcttgc aggataaacc atttatccat
     4921 caccagaccg tcctgatgcc acttatcgtc gtactcctgc atcagcgcgt cacggcaagg
     4981 cagctgtgca gcaaccgccg cagaaagcgc tgccagcgca tcggtcatat tgtttgcttc
     5041 atggtattgc ttgctcacca gcacgtcagc cagatgcgtt tcaccaaaag caaggaagcg
     5101 caggcaggca ttacgcagag tgcgttttgc aatatcttca tgctcaacac ggtactcgct
     5161 ctggtaattc gcgttgtaaa tagccagcag ctcatccgcc agttcagtcg ccagagtacg
     5221 agtgagtgct tcgcgtactt cggcaatagc aatcgggtcg atgatatcga acagttcagc
     5281 catttcattg acagaaggca gcgtcaggat ttctgccgcc agcgccggat caatcttctc
     5341 atcgagcagc accgcgcgga aagcgtcagc cacatgtacc ggcagagaca gcggctgccc
     5401 ttgctgatga cgggcgacgt tcagcttgat gtaggttgcc agcaaacttt gcgccgcatc
     5461 ccaacgggag aaatcattac gcgcatgacg catcaggaag gtcagttgct gatcgctcca
     5521 cttatattcc agtttcactg gcgcagagaa ttcgcacagc agcgcaggca ccggctggaa
     5581 gtagacatta tcaaaaacaa aggtctgttc cgcctgggtg acgttcagca cggaattcac
     5641 cggatgaccg cctttctgca acgggatcac tttgccttcg ttgtcataca attcgatggc
     5701 aaatggaata tgcagcggct gtttttctgc ctgatccggc gtggctggcg tgcgctggct
     5761 gatggtcagg gtgcactgct cggtttccgg attgtagtcg tctttgacgg tcacaatcgg
     5821 cgtgccggac tggctgtacc aacggcggaa atgggagaga tcgacattcg acgcatcttc
     5881 catcgcctgc acaaagtcgt cgcaggttgc cgcgctgcca tcatggcgct cgaaatagag
     5941 ttgcatccct ttctggaagt tttcttcgcc cagcagggtg tggatcatgc gaatcacttc
     6001 cgcgcccttc tcgtaaacgg tcagggtgta gaagttgttc atctcaatga ccatatccgg
     6061 gcggatcggg tgcgccatcg ggctggcgtc ttccgcaaac tgcaatccgc gcatggtgcg
     6121 cacattattg atacggttta ccgcgcggga accaaggtca gagctgaatt cctgatcgcg
     6181 gaagacggtt aaaccttctt tcaggctgag ctggaaccag tcgcggcagg tcactcggtt
     6241 accggtccag ttatggaaat attcatggcc gataacgcgt tcaatatcga ggtaatcttt
     6301 gtcagtggcg gtgtcggtgc gggccagcac atatttggag ttaaagatat tcagcccctt
     6361 attctccatt gcgcccatat tgaagaaatc caccgcgacg atcatataga tgtcgaggtc
     6421 atactccagg ccgaagcgtt cttcatccca tttcattgag tttttcagcg aggtcatcgc
     6481 ccacggcgcg cgatcaaggt tgccgcgatc gacgtacagc tccagcgcca cttcgcgacc
     6541 agaacgcgtg gtaaaggtgt cgcgcagtac atcaaagtcg cctgccacca gcgcaaacag
     6601 gtagcacggt ttcgggaacg ggtcctgcca ctgtacccaa tggcgtccgt tttccagttc
     6661 accttgcgca acgcggttac cgttggaaag caggaaggga tatttggttt tatcggcaat
     6721 aattttggtg gtaaaacgcg ccagcacgtc cgggcggtcg agataatacg taatatggcg
     6781 gaaaccttcg gcttcacact gggtgcaaag cgcatcgcct gactgataaa gcccttccag
     6841 cgccgtattc gccgccgggc ttatttcatt aatgatctta agcgtaaaac gctccggcaa
     6901 attactgatg accagtgcgc cctcttcttc tttccaggcg gtccacggct catcattaat
     6961 atgaacagaa accagtttga ggtcttcgcc gttgagacga agcggagcat ctgatgcacc
     7021 atgacggaca gcctggctga ccgcggtaac gaccgtcttt tgcgcgtcga ggtcaaaggt
     7081 caagtcaata tcagtaatct ggtaatccgg cgcacgataa tcgtgacggt atttggcttg
     7141 tggctgttga gtcataaata acctttagca tcttttatag agtctggtgt tcagtctatt
     7201 cctgttgcgt aaatcgcgct atgcagaatc ttcatctttt caggtacaaa cgcctttatt
     7261 gctacatttt tataacatac accgcgtaat gccatcgacc agaaaggtgg catatggtgt
     7321 gatcggggtt caataaattg cgaaacaagg tatactccag cagttcctga agatgtttat
     7381 tgtactaaac gctcctgtac gaggacgcta ctgcgcacct atgacacaat tcgcttctcc
     7441 tgttctgcac tcgttgctgg atacagatgc ttataagttg catatgcagc aagccgtgtt
     7501 tcatcactat tacgatgtgc atgtcgcggc ggagtttcgt tgccgaggtg acgatctgct
     7561 gggtatttat gccgatgcta ttcgtgaaca gattcaggcg atgcagcacc tgcgcctgca
     7621 ggatgatgaa tatcagtggc tctctgccct gcctttcttt aaggccgact atcttaactg
     7681 gttacgcgag ttccgcttta acccggaaca agtcaccgtg tccaacgata atggcaagct
     7741 ggatattcgc ttaagcggtc cgtggcgtga agtcatcctc tgggaagttc ctttgctggc
     7801 ggttatcagt gaaatggtac atcgctatcg ctcaccgcag gccgacgttg cgcaagccct
     7861 cgacacgctg gaaagcaaat tagtcgactt ctcggcgtta accgccggtc ttgatatgtc
     7921 gcgcttccat ctgatggatt ttggcacccg tcgccgtttt tctcgcgaag tacaagaaac
     7981 catcgttaag cgtctgcaac aggaatcctg gttcgtgggc accagcaact acgatctggc
     8041 gcgtcggctt tccctcacgc cgatgggaac acaggcgcac gaatggttcc aggcacatca
     8101 gcaaatcagc ccggacctag ccaacagcca gcgagctgca cttgctgcct ggctggaaga
     8161 atatcccgac caacttggca ttgcattaac cgactgcatc actatggatg ctttcctgcg
     8221 tgatttcggt gtcgagttcg ccagtcggta tcagggcctg cgtcatgact ctggcgaccc
     8281 ggttgaatgg ggtgaaaaag ccattgcaca ttataaaaag ctgggaattg atccacagag
     8341 taaaacgctg gttttctctg acaatctgga tttacgcaaa gcggttgagc tataccgcca
     8401 cttctcttcc cgcgtgcaat taagttttgg tattgggact cgcctgacct gcgatatccc
     8461 ccaggtaaac cccctgaata ttgtgattaa gttggtagag tgtaacggta aaccggtggc
     8521 gaaactttct gacagccctg gcaaaactat ctgccatgat aaagcgtttg ttcgggcgct
     8581 gcgcaaagcg ttcgaccttc cgcatattaa aaaagccagt taatatcatc agggagctaa
     8641 tcggctccct ttttttacct ttaattccga aatctttcgc tgcatttgcg aattctgctt
     8701 gtctgattgc agatgccagg taacataggt atccccccat ttacgggtgg acaagtgttt
     8761 attttttccg actattaaca gagagaatat tatgagcgtt gtgcctgtag ccgacgtact
     8821 ccagggccgc gtagccgttg acagcgaagt caccgtacgc ggatgggtac gtacccgccg
     8881 agattcaaaa gctggcatct ccttcctcgc cgtttatgac ggttcctgct ttgatcctgt
     8941 acaggctgtc atcaataatt ctctgcccaa ttacaatgaa gacgtcctgc gtctgaccac
     9001 cggctgctcg gtcattgtga cgggtaaagt cgtggcgtcg ccgggccagg ggcaacaatt
     9061 tgaaattcag gccagcaaag ttgaagttgc tggttgggtt gaagatccag acacttaccc
     9121 gatggcggca aaacgccaca gcattgagta tctgcgtgaa gtcgctcacc tgcgtccgcg
     9181 cacaaacctg attggtgccg tcgcgcgcgt tcgccatacg ctggcgcagg cgctgcatcg
     9241 cttctttaac gagcagggat tcttctgggt ttcaacgcca ctgattaccg catctgatac
     9301 cgaaggtgca ggcgaaatgt tccgcgtttc tacgctggat ctggaaaacc tgccgcgtaa
     9361 cgatcagggc aaagtggatt tcgacaaaga cttctttggt aaagagtctt tcctgaccgt
     9421 atctggccag ttgaacggcg aaacctacgc ttgcgcatta tccaaaattt ataccttcgg
     9481 cccgactttc cgtgctgaaa actccaacac cagccgtcac ctggcggaat tctggatgct
     9541 ggagccggaa gtggcgtttg ctaacctgaa cgatatcgcg ggtctggctg aagccatgct
     9601 gaaatatgtc ttcaaagcgg ttctcgaaga acgcgcagac gacatgaaat tcttcgctga
     9661 acgcgtagat aaagatgccg tttcacgtct ggaacgcttc attgaggccg atttcgcgca
     9721 ggtggattac accgacgcag tgaccattct cgaaaactgc ggcaggaagt ttgaaaaccc
     9781 ggtttactgg ggcgtcgatc tctcttctga gcatgagcgt tatctggcgg aagaacactt
     9841 taaagcaccg gtagtggtta aaaactatcc gaaagatatt aaagcgttct atatgcgcct
     9901 caacgaagac ggtaaaaccg ttgcggctat ggacgttctg gctccgggca tcggtgagat
     9961 cattggtggc tcccagcgtg aagaacgtct ggacgttctg gacgagcgta tgctggaaat
    10021 gggcctgaat aaagaagatt actggtggta tcgcgatctg cgtcgctacg gtactgttcc
    10081 gcattcaggt ttcggtcttg gttttgaacg cctgattgct tacgtaactg gcgtgcaaaa
    10141 cgtgcgtgat gtgattccgt tcccacgtac tccgcgcaac gccagcttct gattttcttc
    10201 ctcaaagagc cagcgcctgc tggctctttt ttatcctttc tgtcaagtta tctgtttgtt
    10261 aagtcaagca atctatttgc aaccccgcca taacgatcat cctgttacgg aatattacat
    10321 tgcaacattt acgcacaaaa aataacccgc attcttattg cggattagtt ttttcttagc
    10381 tgatagcaca attttcatac tattttttgg cattctggat gtctgaaaga ggattttgtg
    10441 ccaggtcgat aaagtttcca tcagaaacaa aatttccgtt tagttaattt aaatataagt
    10501 aaatcatat
//
LOCUS       DAPHJE010000141        10407 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPHJE010000141 DAPHJE010000000
VERSION     DAPHJE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.142"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..351)
                     /locus_tag="RH222_003839"
     CDS             complement(<1..351)
                     /locus_tag="RH222_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV3798788.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENAST"
     gene            561..1631
                     /gene="aroF"
                     /locus_tag="RH222_003840"
     CDS             561..1631
                     /gene="aroF"
                     /locus_tag="RH222_003840"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV3798789.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            1642..2763
                     /gene="tyrA"
                     /locus_tag="RH222_003841"
     CDS             1642..2763
                     /gene="tyrA"
                     /locus_tag="RH222_003841"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV3798790.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2806..3966)
                     /gene="pheA"
                     /locus_tag="RH222_003842"
     CDS             complement(2806..3966)
                     /gene="pheA"
                     /locus_tag="RH222_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV3798791.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMPLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(3988..4113)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(4065..4112)
                     /gene="pheL"
                     /locus_tag="RH222_003843"
     CDS             complement(4065..4112)
                     /gene="pheL"
                     /locus_tag="RH222_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV3798792.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            complement(4216..4557)
                     /gene="raiA"
                     /locus_tag="RH222_003844"
     CDS             complement(4216..4557)
                     /gene="raiA"
                     /locus_tag="RH222_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV3798793.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(4828..5565)
                     /gene="bamD"
                     /locus_tag="RH222_003845"
     CDS             complement(4828..5565)
                     /gene="bamD"
                     /locus_tag="RH222_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV3798794.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5700..6680
                     /gene="rluD"
                     /locus_tag="RH222_003846"
     CDS             5700..6680
                     /gene="rluD"
                     /locus_tag="RH222_003846"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV3798795.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            6677..7408
                     /gene="pgeF"
                     /locus_tag="RH222_003847"
     CDS             6677..7408
                     /gene="pgeF"
                     /locus_tag="RH222_003847"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV3798796.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            7538..10111
                     /gene="clpB"
                     /locus_tag="RH222_003848"
     CDS             7538..10111
                     /gene="clpB"
                     /locus_tag="RH222_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV3798797.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         2549 a         2442 c         2775 g         2641 t
ORIGIN      
        1 tgtgctggca ttttcatcag cccctttttg ccatacttca tgcagttggc tggcattttc
       61 aacattgttg cagctggcag gaaagctttt accggaaagt cctcgggcat aagtaaaatc
      121 ggtctggcaa gttttctttt gtccttcggc atatccttta aggtatagac cgcgatccgc
      181 ctgtgaatcg ctaaatgcat cgtcatcttt tatggcgctg cccgatatcg catcttccat
      241 accaacatca taccagtcgg tgctggtcaa ggttggcgcg tgagtataag gatcaatctg
      301 acatccgcta accagtaaag ccaacaaggg ggcgataaac tttttcatca tttctttctc
      361 ctttttcaaa gcatagcgga ttgttttcaa agggagtgta aatttatcta tacagaagta
      421 agggttgaaa tcgcgactaa attgcctgtg taaataaaaa tgtacgaaat atggattgaa
      481 aactttactt tatgtgttat cgttacgtca tcctcgctga ggatcaacta tcgcaaacga
      541 gcataaacag gatcgccatc atgcaaaaag acgcgctgaa taacgtacat attaccgacg
      601 aacaggtttt aatgactccg gaacaactga aggccgcttt tccattgagc ctgcaacaag
      661 aagcccagat tgctgactcg cgtaaaacca tttcagatat tatcgccggg cgcgatcctc
      721 gtctgctggt agtatgtggt ccttgttcca ttcatgatcc ggaaactgct ctggaatatg
      781 ctcgtcgatt taaagccctt gccgcagagg tcagcgatag cctctatctg gtaatgcgcg
      841 tctattttga aaaaccccgt accactgtcg gctggaaagg gttaattaac gatccccata
      901 tggatggctc ttttgatgta gaagccgggc tgcagatcgc gcgtaaattg ctgcttgagc
      961 tggtgaatat gggactgcca ctggcgacgg aagcgttaga tccgaatagc ccgcaatacc
     1021 tgggcgatct gtttagctgg tcagcaattg gtgctcgtac aacggaatcg caaactcacc
     1081 gtgaaatggc ctccgggctt tccatgccgg ttggttttaa aaacggcacc gacggcagtc
     1141 tggcaacagc aattaacgct atgcgcgccg ccgcccagcc gcaccgtttt gttggcatta
     1201 accaggcagg gcaggttgcg ttgctacaaa ctcaggggaa tccggacggc catgtgatcc
     1261 tgcgcggtgg taaagcgccg aactatagcc ctgcggatgt tgcgcaatgt gaaaaagaga
     1321 tggaacaggc gggactgcgc ccgtctctga tggtagattg cagccacggt aattccaata
     1381 aagattatcg ccgtcagcct gcggtggcag aatccgtggt tgctcaaatc aaagatggca
     1441 atcgctcaat tattggtctg atgatcgaaa gtaatatcca cgagggcaat cagtcttccg
     1501 agcaaccgcg cagtgaaatg aaatacggtg tatccgtaac cgatgcctgc attagctggg
     1561 aaatgaccga tgccttgctg cgtgaaattc atcaggatct gaacgggcag ctgacggctc
     1621 gcgtggctta agaggtttat tatggttgct gaattgaccg cattacgcga tcaaattgat
     1681 gaagtcgata aagcgctgct gaatttatta gcgaagcgtc tggaactggt tgctgaagtg
     1741 ggcgaggtga aaagccgctt tggactgcct atttatgttc cggagcgcga ggcatctatg
     1801 ttggcctcgc ggcgcgcaga ggcggaagct ctgggtgtac cgccagatct gattgaggat
     1861 gttttgcgtc gggtgatgcg tgaatcttac tccagtgaaa acgacaaagg atttaaaaca
     1921 ctttgtccgt cactgcgtcc ggtggttatc gtcggcggtg gcggtcagat gggacgcctg
     1981 ttcgagaaga tgctgacact atcgggttat caggtgcgga ttctggagca acatgactgg
     2041 gatcgagcgg ctgatattgt tgccgatgcc ggaatggtga ttgttagtgt gccaatccac
     2101 gttactgagc aagttatcgg caaattaccg cctttaccga aagattgtat tctggttgat
     2161 ctggcatcag tgaaaaatgg accattacag gccatgctgg cggcgcacga tggcccggta
     2221 ctggggttac acccgatgtt cggcccggac agcggtagcc tggcaaagca agttgtggtc
     2281 tggtgtgatg gacgtaagcc ggaagcatac caatggtttc tggagcaaat tcaggtctgg
     2341 ggcgctcggc tgcatcgtat tagcgctgtc gagcacgatc agaatatggc gtttattcag
     2401 gctctgcgcc actttgctac ttttgcttat gggctgcatc tggcagaaga aaatgttcag
     2461 cttgagcaac ttctggcgct ctcttcgccg atttaccgcc ttgagctggc gatggtcggg
     2521 cgactgtttg ctcaggatcc gcagctttat gccgacatta ttatgtcgtc agagcgtaat
     2581 ctggcgttaa tcaaacgtta ctataagcgt ttcggcgagg cgattgagtt gctggagcag
     2641 ggcgataagc aggcgtttat tgacagtttc cgcaaggtgg agcactggtt cggcgattac
     2701 gcacagcgtt ttcagagtga aagccgcgtg ttattgcgtc aggcgaatga caaccgccag
     2761 taataatcca gtgccggatg attcacatca tccggcacct tttcatcagg ttggatcaac
     2821 aggcactacg ttctcacttg ggtaacagcc caataccttc attgaacggg taatttcccc
     2881 taactctttc aatgcttttt gcatttccgc tgattcaaga ttggcctgaa tatccagata
     2941 aaacatctct tcccatggat taccgtgaat cgggcgtgat tccagacggg tcataatcag
     3001 attgtggttg cgcagcacca gcaacgcttc aaccagcgca ccggcttgtt gcccggtcgc
     3061 cattaacaac gtcgttttcg ccggaacctg atcagacacg ttaatggctt tacgcgccaa
     3121 caccacaaat cgggtgaagt tttgtcgctg attcgcttca atacgctcca gtacctgcaa
     3181 accgtacaaa gtgccgccag cttcgcttcc caacgcagca acatgcggtg attttgcctg
     3241 tgcaaccttt tccattgccg cagacgtact ttcggtatat tcaatcttcc agtgcggata
     3301 acgattaagg aatttgctgc attgctggaa cggctgtgga tgactgtaga ccgtgttgat
     3361 ggtagataaa tctgtggtgc cggaaaccag caggcagtgg tcgatggtaa ggggcatttc
     3421 gcccacaatc gacaagcagg tatgttgcag cagatcgtaa acgtcgttta tggcaccgga
     3481 gctggtattt tcaatcggta cgacggcata gtcggcctgg ccggtttcca cctgattaaa
     3541 aatatcggca aatttggcgc agccactttc aatgaattgc tcaaagtgac gggcagcgta
     3601 ctgacgagcg gcaagatgtg aataggagcc tttggggccg agaaaagcga tgcgtgctga
     3661 gtgcggatta attttattga gatgttgttg gagcaaagcc tgctgagtta atacggaatc
     3721 ttcaatgatg agctggaaca ggcgagtaat gtaatgggcg tccagatggt gcgctttacc
     3781 gagcgtaatt aatctttcca gtaaatcgcg ttcacgatca atatcacgta ccgggcgatg
     3841 cgagagcagt ttggcttttc ccacctcgac ggccagttcg cgccgctctg cgagtaatgc
     3901 taataatttt tcatccagcg cgctgatttt ctctcgcagc gccagtaacg ggttttccga
     3961 tgtcatagtg ttgccttttt gttatcaata aaaaaggccc cccgatttgg gaggccttat
     4021 tgttcgtctt cgcattctgt ttcacacgac gaaacgcctc cctttcaggg gaagataaaa
     4081 aagaatgcga agaaaaacgg tgtgtgtttc atgtttgttt ccttaagtga ctttagtaca
     4141 gtacccgtac tgttttcacg ctgtcaacaa aaaacgcgcc cgaaggcgcg ttggcgatac
     4201 actcaatata aaggactact cttcttcaac ttcttcgacg aagttggcgt ctttcaccga
     4261 tgttgcggca cgacgtgctt cgcctttgtg ctgcagttta ttgagctgcc gttccagctt
     4321 gttgatcaat tcgttaattg cggtgtacat atcttcatgt ttaccactgg caaccagaac
     4381 gccgttaggt gtattgattg tggcgtcagc aacaaaccct tgtggctctt tggacagaat
     4441 gatatgtgga ttaatcagat gtgtttgcca tttttccagt ttggcgagac ggtctgcgac
     4501 atgttggcgg atggccggag taatttccat ttgtttgctg gtaatgttca ttgtcataaa
     4561 ttttacctct tgtcttcccg tcttggtgaa ttcagcatac cgttcctgat gtcaaaatgt
     4621 gtgatgaaaa tctcataatt ttgtcacttt ttgtcaacga atcttttttg tgagaaatcg
     4681 caggaagagg tgttttttta cttgaggaat atctcaaagc gagccatatt tgatgagatc
     4741 gatagcgact aaatcggttc agtttcacaa ctgacagaat aaaaaaacgg cagctcaagg
     4801 gctgccgttt tgtgtttcag gtttctgtta tgtattgctg ctgtttgcgg cgatgatttt
     4861 cgctactttt tcagcttgcg cattcatctg catctgacgg tatgcatttt ccatcagcgg
     4921 cagcgcatca cgcgtagcct gggtatccgg gtagtcgcgc aacatgcctt ctacgcggtt
     4981 aacgacggca acccatgcgc cacgttctgt atagtactcg gccacggagt attcatattt
     5041 cgccagacga tctttcagga ataccagacg tttggtggca tcggtggtgt actgactgtt
     5101 cggatagccg cgcaccagtt tggaaaagtc actaaacgca gctcgtgcat gttgaggatc
     5161 gcgatcgcta cgatcgacgc caaagaaccc ttgcagcgca ctgtcatcca gcgccatatt
     5221 ggtcaggcca cgcatgtaca tgacataatc gatattcgga tgggtcgggt taaggcgaat
     5281 aaaacgatcg atggcagcct gtgctaacgg caaatcggcg tttttatagt aggcgtagat
     5341 gagatccagc tgcacctgct gcgaatacgg accaaacgga tagcgattat ctaacgcttc
     5401 cagttgcgtt attgcctgtc tccagttacc gtcctgcagc ttttgttgtg cagtcgcgta
     5461 aatttcattt ggcggattat caggtacttc ttcctttgac cccgagcaac ccgccaaaaa
     5521 caggcttagt gtggcggctg ccaccagata tttcatgcgc gtcatgacgt tttgactttc
     5581 ctcaaaatgt aatacgggag attctctgtt cctgctcccg gttaagacca gctacaatag
     5641 cacactatat taaacggcaa agccgtaaaa ccccaacgat aaacgaagaa gcagtatata
     5701 tggcacaacg agtacagctc actgcaacgg tgtccgaaaa ccaactcggt caacgcttag
     5761 atcaggcttt ggccgaaatg ttcccggatt attcacgttc gcgaataaaa gaatggatcc
     5821 tcgaccagcg agtgctggtt aacggcaaag tttgtgataa gccgaaagaa aaagtattgg
     5881 gtggcgagca ggttgccatc aacgctgaga ttgaagaaga agcgcgtttt gaaccgcagg
     5941 atatcccgct ggatatcgtc tatgaagatg aagacattat tatcattaat aaaccgcgcg
     6001 acctggtggt acatcctggc gcgggtaacc cggatggcac ggtactgaat gcgttgcttc
     6061 attactatcc acccattgcc gatgtaccgc gtgcgggcat cgtccatcgt ctggataaag
     6121 acaccactgg cctgatggtt gtggcaaaaa ccgttccggc tcagacgcgt ttagtcgaat
     6181 ctttgcaacg gcgtgaaatt actcgtgagt atgaagcggt ggcgattggt catatgaccg
     6241 caggtggcac ggtggacgag ccaatcagtc gccacccgac caaacgtacc catatggcgg
     6301 tgcatccgat gggcaaacca gcggtgactc actatcgcat catggaacac ttccgtgtgc
     6361 acacgcgtct gcggttgcgt ctggaaactg gacgtacgca ccagatccgc gtgcatatgg
     6421 cccatatcac tcatccgctg gtgggcgatc cggtttatgg tggccgtccg cgtccgccaa
     6481 aaggtgcttc ggaagcattt atctccacgc tgcgtaagtt tgaccgccag gcgctacatg
     6541 caaccatgct gcgtctttat cacccgatct ccggcatcga aatggaatgg catgcgccta
     6601 ttccacaaga tatggtggag ctgattgagg tgatgcgcgc cgatttcgaa gaacataagg
     6661 atgaagtgga ctggttatga gtaagctgat tgtcccgcag tggccgctgc caaagggtgt
     6721 tgcggcttgt agctctactc gtatcggcgg cgtgagcttg cccccgtatg actcgctcaa
     6781 cctcggtgcc cattgtggcg ataacccgga tcacgttgag gagaatcgca agcgactttt
     6841 tgctgcgggc aatttgcctt ctaaaccggt ctggcttgag caggtacacg gcaaagatgt
     6901 gcttaagctt actggcgaac cttatgcctc aaaacgagca gatgcctctt atagcaatac
     6961 gcctggcacg gtttgcgcag tgatgactgc cgactgcctc cctgtgctgt tttgcaatcg
     7021 agcgggaacg gaagtcgccg ccgctcatgc tggctggcgt ggactgtgcg caggcgtgct
     7081 ggaagagaca gtttcctgtt ttgctgataa tccggaaaat attctcgcct ggttagggcc
     7141 ggcaattggt ccacgcgcgt tcgaagtggg ggcggaggtt cgcgaggcgt ttatggcagc
     7201 agacgccaaa gcgagtacag ctttcattca gcatggtgat aagtatctgg cggatattta
     7261 tctgcttgcc cggcagcgtc tggcgagcgt tggtgttgaa caaattttcg gcggcgaccg
     7321 ttgtacatat acggaaaatg agactttctt ctcttatcgt cgcgacaaga ccaccgggcg
     7381 tatggcaagt ttcatttggc tgatataacc taaagaatca agacgatccg gtacgcgtga
     7441 ttttcttttc acattaatct ggtcaataac cttgaataat tgagggatga cctcatttaa
     7501 tctccagtag caactttgat ccgttatggg aggagttatg cgtctggatc gtcttactaa
     7561 taaattccag cttgctcttg ccgatgccca atcacttgca ctcgggcacg acaaccaatt
     7621 catcgaacca cttcatttaa tgagcgccct gctgaatcag gaagggggtt cggttagtcc
     7681 tttgttaaca tcagctggca taaatgctgg ccagttgcgc acagatatca atcaggcatt
     7741 aaatcgttta ccgcaggttg aaggtactgg tggtgatgtc cagccatcac aggatctggt
     7801 gcgcgttctt aatctttgcg acaagctggc gcaaaaacgt ggtgataact ttatctcgtc
     7861 agaactgttc gttctggcgg cacttgagtc tcgcggcacg ctggccgaca tcctgaaagc
     7921 agcaggggcg accaccgcca acattactca agcgattgaa caaatgcgtg gaggtgaaag
     7981 cgtgaacgat caaggtgctg aagaccaacg tcaggctttg aaaaaatata ccatcgacct
     8041 taccgaacga gccgaacagg gcaaactcga tccggtgatt ggtcgtgatg aagaaattcg
     8101 ccgtaccatt caggtgctgc aacgtcgtac taaaaataac ccggtactga ttggtgaacc
     8161 cggcgtcggt aaaactgcca tcgttgaagg tctggcgcag cgtattatca acggcgaagt
     8221 gccggaagga ttgaaaggcc gccgcgtact ggcgctggat atgggcgcgc tggtggctgg
     8281 ggcgaaatat cgcggtgagt ttgaagaacg tttaaaaggc gtgcttaacg atcttgccaa
     8341 acaggaaggc aacgtcatcc tgtttatcga cgaattacat accatggtcg gcgcgggtaa
     8401 agccgatggc gcaatggacg ccggaaacat gctgaaaccg gcgctggcgc gtggtgaatt
     8461 gcactgcgta ggtgccacga cgcttgacga atatcgccag tacatcgaaa aagatgctgc
     8521 gctggaacgt cgtttccaga aagtgtttgt tgccgagcct tctgttgaag ataccattgc
     8581 gattctgcgt ggcctgaaag aacgttacga attgcaccac catgtgcaaa ttactgaccc
     8641 ggcaattgtt gcagcggcga cgttgtctca tcgctacatt gctgaccgtc agctgccgga
     8701 taaagccatc gacctgatcg atgaagcagc atccagcatt cgtatgcaga ttgactcaaa
     8761 accagaagaa ctcgaccgac tcgatcgtcg tatcatccag ctcaaactgg aacaacaggc
     8821 gttaatgaaa gagtctgatg aagccagtaa aaaacgcctg gatatgctca acgaagaact
     8881 gagcgacaaa gaacgtcagt actccgagtt agaagaagag tggaaagcag agaaggcatc
     8941 gctttctggt acgcagacca ttaaagcgga actggaacag gcgaaaatcg ctattgaaca
     9001 ggctcgccgt gtgggggacc tggcgcggat gtctgaactg caatacggca aaatcccgga
     9061 actggaaaag caactggaag ccgcaacgca gctcgaaggc aaaactatgc gtctgttgcg
     9121 taataaagtg accgacgccg aaattgctga agtgctggcg cgttggacgg ggattccggt
     9181 ttctcgcatg atggaaagcg agcgcgaaaa actgctgcgt atggagcaag aactgcacca
     9241 tcgcgtcatt ggtcagaacg aagcggttga tgcggtatct aacgctattc gccgtagccg
     9301 tgcggggttg gcggatccaa atcgcccgat tggttcattc ctgttcctcg gcccaacggg
     9361 tgtggggaaa acagagcttt gtaaggcgct ggcgaacttt atgtttgata gcgacgaggc
     9421 gatggtccgt atagatatgt ccgagtttat ggagaaacac tcggtgtctc gtttggttgg
     9481 tgcgcctccg ggatatgtcg gttatgaaga aggtggctac ctgaccgaag cggtgcgtcg
     9541 tcgtccgtat tccgtcatcc tgctggatga agtggaaaaa gcgcatccgg atgtcttcaa
     9601 cattctgttg caggtactgg atgatgggcg tctgactgac gggcagggga gaacggtcga
     9661 cttccgtaat acggtcgtca ttatgacctc taacctcggt tccgatctga ttcaggaacg
     9721 cttcggtgaa ctggattatg cgcacatgaa agagctggtg ctcggtgtgg ttagccataa
     9781 cttccgtccg gaattcatta accgtatcga tgaagtggtg gtcttccatc cgctgggtga
     9841 acagcacatt gcgtcgattg cgcagattca gttgaaacgt ctgtataaac gtctggaaga
     9901 acgtggttat gaaatccaca tttctgacga ggcgctgaaa ctgctgagcg agaacggtta
     9961 cgatccggtc tatggtgcac gtcctctgaa acgtgcaatt cagcagcaga tcgaaaaccc
    10021 gctggcacag caaatactgt ctggtgaatt ggttccgggt aaagtgattc gcctggaagt
    10081 taatgaagac cggattgtcg ccgtccagta aatgataaaa cgagcccttc ggggctcgtt
    10141 tttgtctata agttagacgg aaaagactat atttaagatt ttttgcctga aaaatgagcg
    10201 aacgataaag tttttatctt tttcgcttgt caggccggaa taactcccta taatgcgcca
    10261 ccactgacac ggaacaacgg caaacacgcc gccggggcag cggggttctc ctgagaatct
    10321 caacagagaa aagcaaaaaa aatgcttgac tctgtagcgg gaaagcgtat tatgcatacc
    10381 ccgcgccgct gggaaaaagc gaagcgg
//
LOCUS       DAPHJE010000142        10155 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPHJE010000142 DAPHJE010000000
VERSION     DAPHJE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.143"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..350
                     /gene="bdm"
                     /locus_tag="RH222_003849"
     CDS             135..350
                     /gene="bdm"
                     /locus_tag="RH222_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV3798798.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            452..589
                     /gene="sra"
                     /locus_tag="RH222_003850"
     CDS             452..589
                     /gene="sra"
                     /locus_tag="RH222_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV3798799.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            746..2443
                     /gene="maeA"
                     /locus_tag="RH222_003851"
     CDS             746..2443
                     /gene="maeA"
                     /locus_tag="RH222_003851"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV3798800.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVHNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2577..3587
                     /gene="adhP"
                     /locus_tag="RH222_003852"
     CDS             2577..3587
                     /gene="adhP"
                     /locus_tag="RH222_003852"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV3798801.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVCTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3733..4017
                     /locus_tag="RH222_003853"
     CDS             3733..4017
                     /locus_tag="RH222_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV3798802.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4424..5077)
                     /gene="fdnI"
                     /locus_tag="RH222_003854"
     CDS             complement(4424..5077)
                     /gene="fdnI"
                     /locus_tag="RH222_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV3798803.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5070..5954)
                     /gene="fdnH"
                     /locus_tag="RH222_003855"
     CDS             complement(5070..5954)
                     /gene="fdnH"
                     /locus_tag="RH222_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV3798804.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5967..9014)
                     /gene="fdnG"
                     /locus_tag="RH222_003856"
     CDS             complement(5967..9014)
                     /gene="fdnG"
                     /locus_tag="RH222_003856"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8427..8429),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV3798805.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQSEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9246..10127
                     /gene="yddG"
                     /locus_tag="RH222_003857"
     CDS             9246..10127
                     /gene="yddG"
                     /locus_tag="RH222_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV3798806.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2469 a         2649 c         2575 g         2462 t
ORIGIN      
        1 ggtgttaaaa tataaaaatc atcaaccagg actaatctta acaacgaagc ggcaaatatt
       61 tgccatgctg aatgtgcttt ataaatctgc gatccgtagc agacaccata aatacacaga
      121 cacggagaat cactatgttt acttattatc aggcagaaaa ttcaacagca gaacccgctc
      181 tggttaatgc cattgagcaa ggccttcggg cacagcacgg tgttgtcact gaagatgaca
      241 ttctcatgga gctgaccaaa tgggttgaag cttctgataa cgacatcctc agtgatatct
      301 accagcaaac gatcaattat gtggtcagtg gccagcaccc tacgctttaa ggtgctatgc
      361 ttgatcggca acctaattta ggggtttagc acgtgtttct tcgctacggc gatgttgtcc
      421 ttaaaactag caacaggatt gaggagttaa aatgaaatcg aaccgtcagg cacgtcatat
      481 tcttggactg gaccataaaa tttctaacca gcgcaaaata gttaccgaag gtgacaaatc
      541 cagcgtggta aataacccaa ccggcagaaa acgccccgct gaaaagtaat tcataaccat
      601 cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg gtgtttttgt ttttatctgc
      661 tttatacttg gggccgacgc cctggcggta aagcaaagac gataaaagcg tgccagggat
      721 ggatattcaa aaaagagtga gtgacatgga accaaaaaca aaaaaacagc gttcgcttta
      781 tatcccttac gctggccctg tactgctgga atttccgttg ttgaataaag gcagtgcctt
      841 cagcatggaa gaacgccgta acttcaacct gctggggtta ctgccggaag tggtcgaaac
      901 catcgaagaa caagcggaac gagcatggat ccagtatcag ggattcaaaa ccgaaatcga
      961 caaacacatc tacctgcgta acatccagga caccaacgaa accctcttct accgtctggt
     1021 aaacaatcat cttgatgaga tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg
     1081 tgagcgtttt tctgagatct accgccgttc acgcggcgtg tttatctctt accagaaccg
     1141 gcacaatatg gacgatattc tgcaaaacgt gccgaaccat aatattaaag tgattgtggt
     1201 gactgacggt gaacgtattc tggggcttgg tgaccagggc atcggcggga tgggcattcc
     1261 gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc agcccggcgt atacccttcc
     1321 ggtggtgctg gatgtcggaa cgaacaacca acagctgctt aacgatccgc tgtatatggg
     1381 ctggcgtaat ccgcgtatca ctgacgatga atactatgaa ttcgttgatg aatttatcca
     1441 ggctgtgaaa caacgctggc cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa
     1501 tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt tcttttaacg atgacattca
     1561 gggcactgcg gcagtaacag tcggcacact gatcgcagcc agccgcgcgg caggtggtca
     1621 gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg gcattgccga
     1681 aatgatcatc gcccagaccc agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt
     1741 ctttatggtc gatcgctttg gcctgctgac ggacaagatg ccgaacctgc tacctttcca
     1801 gaccaaactg gtgcagaagc gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt
     1861 gtcactgctg gatgtggtgc acaatgtaaa accagatatt ctgattggcg tctcaggaca
     1921 gaccgggctg tttacggaag agatcattcg tgagatgcat aaacactgtc cgcgtccgat
     1981 cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc acaccgcagg acattatcgc
     2041 ctggaccgaa ggtaacgcgc tggtcgccac tggcagcccg tttaatccag tggtatggaa
     2101 agataaaatc taccctatcg cccagtgtaa caacgccttt attttcccgg gcatcggcct
     2161 gggggttatt gcttccggcg cgtcacgtat caccgatgag atgctgatgt cggcaagtga
     2221 aacgcttgct cagtattcgc cgctggtcct gaacggcgaa ggtctggtac taccggaact
     2281 gaaagatatt cagaaagtct cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca
     2341 gcaaggcgtg gcggtgaaaa catctgccga agctctgcaa caggccattg atgataattt
     2401 ctggcaagcc gaataccgcg actaccgccg tacctccatc taagcctgcg cccggtagtg
     2461 aaggctaccg ggctatttct ctctcccttt ttcagatctc atccatactg ggtagtggcg
     2521 aataaatctc atttgcctca cctgctatgc agaacatcat ccgaaaagga ggaactatga
     2581 aggctgcagt tgttacgaag gatcatcacg ttgacgttac ggataaaaca ctgcgctcac
     2641 tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc
     2701 atgttaagaa tggcgatttt ggtgacaaaa ctggcgtaat tctgggccat gaaggcatcg
     2761 gtgtggtggc agaagttggc ccaggtgtca cctcattaaa accaggcgat cgtgccagcg
     2821 tggcgtggtt ctacgaagga tgcggtcatt gtgaatactg taatagtggt aacgaaacgc
     2881 tctgccgttc agttaaaaat gccggataca gcgttgatgg cgggatggcg gaagagtgca
     2941 tcgtggtcgc cgattacgcg gtaaaagtgc cagatggtct ggactcggcg gcggccagca
     3001 gcattacctg tgcgggggtc accacctaca aagccgttaa actgtcaaaa attcgtccag
     3061 ggcagtggat tgctatctac ggtcttggcg gtctgggtaa cctcgctctg caatacgcga
     3121 agaatgtctt taacgcgaaa gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg
     3181 caaccgaaat gggtgcagat ttagcgatta actcacgcac cgaagacgcc gccaaaattg
     3241 tgcaggagaa aaccggtggc gctcacgctg cggtggtaac agcagttgct aaagctgcgt
     3301 ttaactcggc agttgatgct gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc
     3361 cggagtctat gagcctggat atcccacggc tggtgctgga tggcattgaa gtggtcggtt
     3421 cgctggtctg cacgcgccag gatttaactg aagccttcca gtttgccgcc gaaggtaaag
     3481 tggtgccgaa agtcgccctg cgtccgttag cggacatcaa caccatcttt actgagatgg
     3541 aagaaggcaa aatccgtggc cgcatggtga ttgatttccg tcactaagag gcctttgctg
     3601 cgactgccat gttcgggtcg cagcatcgca cactctccaa catgaaatgg ctaaatggat
     3661 tattgttgcg taggaaggaa caataatgat agtgttccca gtgaaggaac gctactgaga
     3721 gtaaagacac taatgaaaat ggccaatcat ccccgcccgg gggacattat tcaggaatca
     3781 ctggacgaac ttaatgtcag cctgcgcgag tttgccagag caatggaaat tgctccctca
     3841 acggcaagcc gattgctgac aggaaaagca gctttgacgc cagaaatggc gattaaactt
     3901 tccgtggtga tcggcagttc gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg
     3961 gcagaggcag aaaaaacagt ggatgtgtcg cgattacgcc gtctggttac gcaataaaaa
     4021 aatggcactt tataaagcgc catttagaac tgatgacaaa cgcaacattg cctgatgcgc
     4081 tacgcttatc aggcctacac gttcactgca atctattgga tttgaagggg ttgtaggccg
     4141 gataaggcgt tcacgccgca tccggcatga acaaagccta cgttgtctac aatatgaaaa
     4201 tgacgccttt tacagcgcca tttgaaactg atgacaaacg caacattgcc tgatgcgcta
     4261 cgcttatcag gcctacacgt tcactgcaat ctattggatt tgaagggttt gtaggccgga
     4321 taaggcgttc acgccgcatc cgggatgaac aaagcctacg ttgtctacaa tatgaaaatg
     4381 gcgcctttta cagcgccatt ttcagcttaa agttcaaaag agattatatc ccttcttcac
     4441 tctctttttt cgcttctgcc ttctcgattt cacgatacca gcgtggatgg tgtttcttcg
     4501 cccagcgacg gctcaccttc ccttcgatca tccctttaat cgaccctttc acccaaaatg
     4561 ccatatacat atggatcagg atggcgtgga tcaggatgat acccgcagcc gcgtggatca
     4621 gcaggctgta gcgaacaacc tgcatcggga agtactgcgc aaagtacgga cgccagataa
     4681 tcaccccggt caccagcagc acgaaaatca tgctcatgat cgaccagaac atcatctttt
     4741 gcccggcgtt gtacttaccg acatccgcca ctttatgctc attgcctttc aatacttcga
     4801 caatgttcaa cagccacgga atatctttct tatccgggat gttgtgatgc acaaaacgca
     4861 caaacataaa catcagtgcg acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc
     4921 ccatctgcgg cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa
     4981 tcccggacag cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa
     5041 taaatttggt gcgcacaatc attttcgact tactcatgat gatcctcctc gtcatcgtcc
     5101 acttccttat tcgggccaat accgatgtag tggaaaatca acccggcaaa agtggcaata
     5161 aagccagccg ctgccagcgg tttcaacgcg cctttccaca ggcttaccga ggtgtcgatc
     5221 ttcggatctt tcggcagacc gtgatacagc tccggctgat cggcgtgatg cagcacgtac
     5281 ataacgtgcg taccaccgac cccttccggg ttgtagacgc cagcatgttc gtaaccacgc
     5341 gctttcagtt tcgccacgcg ctgttccgcc agctccagca tctccttctt ggtgccgaag
     5401 tggatagccc cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga
     5461 tcgacgcaga gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta
     5521 aacggacacc cggcaatgca gtaaccacag ccgatgcagt tttccgactg gaaatcgaca
     5581 atcccgttag cgtactgaat gattgcacca gcagacgggc acgccttcag gcagccggga
     5641 tcttcacagt gcatacagcc gtctttacgg atcagccact ccagcttgcc gttctgttcg
     5701 gtttcgctaa agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa
     5761 accccgacgc agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga
     5821 caggctttac agccgataca ggtggaaacg tcgataagtt ttgcgacttc tgctttgtaa
     5881 tcacgcacct gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatgtcctgc
     5941 gtttccatag ccatctgttc gcccccttac gccttctcga tgttgactaa gaacgcttta
     6001 tattccggcg tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agcgatataa
     6061 cctttacgcg cgacaccctc aaagccccag tggattggaa tacccaccgt ttcaacctgc
     6121 tgaccattta cattcagcgg tttcagacga cgcgttacca cagccaccgc gcggataaag
     6181 ccacgcttgc tggagacagt gacacgatcg ccattattaa tgcctttcgc cgccgccagc
     6241 gtttcgctga tttccacaaa ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg
     6301 gtccaggtgt ggaagtgctc ggtcagacga taggtcgtac ccacatacgg gaactgctct
     6361 tttttaccca tccgcagcgc gtcttgttca tacagacgaa caaccgggtt agacaccacg
     6421 ttcggatgca gcgggttagt gcccagcggc gtttcaatcg gctcgtagtg ttccgggaac
     6481 ggaccttccg ccattttgtt gatggcaaac aggcgtccca tcccttccgg ctgcatgata
     6541 aacggcccgg ttggcgtacc cggtgcggca ttgccgaagt caggaatatc gttacccgtc
     6601 cacttgctgc cgttccactg gatcagcatc cgtttcggat cccacggttt accgttgata
     6661 tccgccgaag cacggttgta cagcacgcga cggttgagcg gccacgccca ggcccatccc
     6721 agcgtattcc ccagaccgga cgggtcggag ttatcgcggt tagccatctg gttgccctgc
     6781 tctgtccagc taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc atcacgcaga
     6841 tgcgcaaagc tactcagcaa ctgacctttc ttcgcaataa gcacgccatt agcgtcatag
     6901 agatcttcca gcgcgtagcc gttgttctct ttagccactt cgtcagattg cggttcgtgc
     6961 ggctgcttgt agttccagct catcttcatc agcggttcta cgcctttacc accttcggac
     7021 tggtacagct cgcgcagatg atggtagata cccgccagaa tttcaccgtc gttacgcgct
     7081 tcgcccggcg cgtcctgacc tttccagtgc cactgcaacc agcgaccgga gttagcgata
     7141 gaaccatctt cttcagcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagac
     7201 gccggatcga catcgttcga ctcaccgtgg ttctgccaga aggtagaggt ttcagtcacc
     7261 agcggatcga taaccaccat gtacttcagc ttgctcaggc agctcaccac tttgtttttg
     7321 tccgggaagg acgcaaccgg gttaaagccc tggcagaaat aaccggtgac tttgccttca
     7381 tccatcatgt tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtca
     7441 tagccccagt tgttctcttt ctgcgcggca tcgccataga aagatttcat caggctaacg
     7501 aagaacttcg gatagttgct ccagtagttc acctgatcag ccagcgtcgc tttcggcgtg
     7561 ttcgcttcca gatacgactg caaatcaacc tgtttttctg acggcagcgt cagataacct
     7621 ggcaggctgg tagagagcag gcctaagtca gtcaagccct gaatgttgga gtgaccacgc
     7681 aatgcgttca cgccgccacc ggccataccc atgttaccga gcagcaactg gatcatcgcc
     7741 atagtacgga tgttctgcgc acccacagtg tgctgcgtcc agcccagcgc gtacaggaag
     7801 gtggttgtgc gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg
     7861 gcttttggcg taccgcagat gttttcaacg acgtccggcg tgtagcggga aacgtgctct
     7921 ttcagcaggt tccacacaca gcgcggatga gtcagtgttt catcgcgttt cgcatagccg
     7981 ttttcatcga actgatagtt ccaggacgat ttatcgtatt gacgtttttc agcgtcgtag
     8041 ccgctgaaca gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg
     8101 taatgcttaa cgtattcggc gttgattttg ttgttttcga tcaggtagcg caaaacgcca
     8161 gacaggaacg taatgtccgt accggaacga ataggcgcgt aaatatccgc cacagaagcg
     8221 gtacgcgtaa aacggggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc
     8281 atcgcccagc ggaaaccgac gggatgcgct tcagcagcgt taccgcccat caccatcacg
     8341 acgttagcgt ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     8401 gcaagacttg ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc
     8461 ccgagggagc gggcaaattt ctgggtcagc atcccggttt cgttgctggc accggaggca
     8521 cacagcatac cggtagaaag ccaacggttt accgttacgc cctgctcgtt cttttcaata
     8581 aagttagcgt cacggtcagc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag
     8641 ctaatgcgct gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt
     8701 tcactgttga cgtaatccag caaaccggcc cctttcgggc acagcgcacc acggcttacc
     8761 ggatgatccg ggtcaccttc aatgtgataa atcgcttctc tggcgttttt tgcgccatca
     8821 cccaggctat acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc
     8881 tctttagcgc gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag
     8941 cccaatgccg ctactgttgt tccagccata ccgcccgcgc agattttaaa aaattgtctg
     9001 cgactgacgt ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca
     9061 gtaaaattct gaatcttttt gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat
     9121 ccgcctgcgc aaattgacct acctcaatag cggtagaaaa acgcaccact gcctgacagg
     9181 ccagttaaaa aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag
     9241 cgagcatgac acgacaaaaa gcaacgctca tagggctaat agcgatcgtc ctgtggagca
     9301 cgatggtagg attgattcgc ggtgtcagtg aggggctcgg cccggtcggc ggcgcagctg
     9361 ctatctattc attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc
     9421 aaatcccgaa aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc
     9481 tggcgctttc cttagggtat gcggcgaccc gtcatcaggc gattgaagtg ggtatggtga
     9541 actatctgtg gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca
     9601 actggttgat tgtacctgga ttattattag ccctcgtcgg cgtctgctgg gtgttaggcg
     9661 gtgacaatgg gttacattat gatgaaatca tcaataatat caccaccagc ccattgagtt
     9721 atttcctggc gttcattggt gcgtttatct gggcagccta ttgcacagta acgaataaat
     9781 acgcacgcgg atttaacgga attaccgttt ttgtcctgct aacgggagca tgtctgtgga
     9841 tttactattt tctcacgcca caaccagaaa tggtatttag cacgcccgtc atgattaaac
     9901 tcatctctgc ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatattgc
     9961 atggcaatgt caccataatg gcggtaggct cgtattttac gcctgtactt tcctcagcgc
    10021 ttgcagccgt gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctaatgg
    10081 tctgcggcgg ttccctgctc tgctggctgg cgacacgtcg tggttaaaat aattgccggg
    10141 tctacggccc ggcag
//
LOCUS       DAPHJE010000143        10014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPHJE010000143 DAPHJE010000000
VERSION     DAPHJE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.144"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..81
                     /locus_tag="RH222_003858"
     tRNA            6..81
                     /locus_tag="RH222_003858"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="RH222_003859"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="RH222_003859"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="RH222_003860"
     CDS             complement(468..1226)
                     /locus_tag="RH222_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV3798807.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /locus_tag="RH222_003861"
     CDS             complement(1234..2337)
                     /locus_tag="RH222_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV3798808.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3528)
                     /locus_tag="RH222_003862"
     CDS             complement(2347..3528)
                     /locus_tag="RH222_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV3798809.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3596..4621)
                     /locus_tag="RH222_003863"
     CDS             complement(3596..4621)
                     /locus_tag="RH222_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3798810.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5052..5273)
                     /locus_tag="RH222_003864"
     CDS             complement(5052..5273)
                     /locus_tag="RH222_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798811.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="RH222_003865"
     CDS             complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="RH222_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV3798812.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKLYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="RH222_003866"
     CDS             complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="RH222_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV3798813.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2665 a         2590 c         2406 g         2353 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatttgat gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggaacggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgctcca ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca
      601 aaccccatct catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca
      781 gaaatttgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact
      961 tcctgccgga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctaccacg
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaacctgaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gtgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaacccaa cgccagcgac tctgccgctt catactgccc
     1561 tttaggcagt gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gattacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta atcccagtga
     2641 ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc
     2701 gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa
     2761 aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc
     2821 gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca
     2881 ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac
     2941 agaaaggact atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg
     3001 cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc
     3061 ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag
     3121 cgggggaata ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt
     3181 atccgaaagt ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa
     3241 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3301 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3361 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc
     3421 aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcac gaaccgtagg
     3481 gttggcaaag gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3541 aagcataacg ctgaagagag cgccgccgtc gcgacgctcg ttacctcaca ccgacttaac
     3601 gcacgggcgg tgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3661 ttttcagcgg gctttctgaa cctacgttac gctcaaaaat ttccgagtag ttacccacct
     3721 gtttgatgat gttataggcc catttattat ccaacttcag atccttgccg taatcgcctt
     3781 ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcaacgt
     3841 tctgggaatt gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa
     3901 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3961 cctccggtaa gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt
     4021 gcgactgatc cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     4081 cagagcgatc gaaagtcact ggtgtgtact tcatattgtt tgccttgaaa tagtcggcaa
     4141 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4201 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4261 cagtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4321 gcaaatccac ttcccctgac tgtaaagccg tgaagcgttc ttttgctgtg agtggggtat
     4381 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4441 caatacctga aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga
     4501 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4561 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4621 taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4681 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcccg atttcgcgtc
     4741 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat
     4801 gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg
     4861 attgttagcg tgagtatatt tctggtggga agtgtgaaag ttaaattgat gcgttatttc
     4921 gaaagcagcg aaataacgcg atataacatg ctaaatcagc agagaattag ccagcagaat
     4981 atagcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc gaaactccgc
     5041 gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc aacagccgct
     5101 gccccgccag caatagcccc agccgtttcc cagttatcct gagtcgtgcc tttcatacag
     5161 gtattggtgt gaaccagacg accctgatcg tcataaaccg gaacgcaggg cgaatcgtga
     5221 gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt catggtgtta
     5281 cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag ctgaatgcca
     5341 ttttatcacc ggaggttata aatcttgcgg aatgaataat gacaaattat gtgaattgta
     5401 aaaatagtga agggagcggg aactatagaa agcttttaaa gagtgagcag aagaatatct
     5461 tattaatttt tatgggtttc actggaaata ataaaggcac ccgaaagcgc ctttatgttt
     5521 ctgatttatc ctttaaagca acggcggatc accacaaaga acaccggtac gaagaagatt
     5581 gccagcaacg ttgcagagac cattcctccc attaccccga tacccacagc gttctgcgcg
     5641 ccactgccgg caccgttact gatagctagc ggtaatacgc cgagaataaa ggcgagagag
     5701 gtcatcagga taggacgcag acgcatacgt actgccatta gtgtcgcttc aacaacacct
     5761 ttaccctctt tctccatgag atctttagcg aactcaacaa tcaaaatagc gtttttggcc
     5821 gacaagccaa ttgtcgttag caagcccacc ataaagtaga cgtcattttt ttgattaaag
     5881 agtgtcgccg ccagcagcac gccgacaatc cctaacggca cgaccaacat aaccgagaca
     5941 ggaattgacc agctttcata gagtgcagca aggcacagga aaacaaccac aaaggaaatt
     6001 gctaccagag cgggagcctg gtttcccgat aagcgttcct gatacgacat acccgtccag
     6061 tcataaccaa tgccagcagg taattttgac gcaaggtttt ccatcaacgc catggcatcg
     6121 ccggaactgg ttcctggcgc ggcttccccc tgaatctcca ttgacggcag accgttgtag
     6181 cgttccagtc gcggagagcc atacacccaa tgtgaagtgg taaaggccga gaatggcacc
     6241 atttcgccgt tggcgctgcg gacataaagt ttatcgacat cttctggcag catacggaat
     6301 ttggcatccg cctgaacata caactttttc acgcgaccac ggtcgatgaa gtcgttaacg
     6361 taagtcccac ccaacgccgt tgaaatggtc tgattgatgt cagaaagtga aacacctaat
     6421 gcctgcgcct tttcctggtc aacttccagt ttaaactgcg cggtgtcttc caggccatta
     6481 ggacgcacgc tgactaagct ggcaggatgt tgcgccgcca taccaagcaa ctggttacgg
     6541 gcctgggtta gggcatcgtg acccagccca gcctgatcaa ttaactcaaa gtcgaaaccc
     6601 gttgccgtgc ccagttcaac aatggctggc atattgaatg gaatgacaaa accgtcgcgg
     6661 atcttgccaa attccatttt ggcacgatgg attaccgctt ccgcactgtt ttcatcacca
     6721 ttacgctctt cccacggttt cagactgacg aaggccatac cggcgttttg tgcctggccg
     6781 ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt tcgctttctc gttcttcaga
     6841 tagtaatccg tcacttgatc caacactttt tgcgtccgct cttgcgtcgc gccagcgggt
     6901 aactgaatca tggtcagaaa gacaccctga tcctcttcag gtaagaagga agacggaaga
     6961 cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat agatcagtaa atatcgtcct
     7021 gtggaaccga ggattttgcc gacgctgttg gtgtagtggt taacgctatg atcgaaggta
     7081 gtattaaacc agccgaagaa accgccctta ttttcgtgat gctcagcaga gacgggttta
     7141 agcagcgttg cacataacgc aggggtaaga atcaatgcca ccagaacaga aagcgccatt
     7201 gccgaaacga tggtgataga gaactggcga taaattgccc cagtagaacc gccgaagaat
     7261 gccatcggaa taaataccgc tgacagcacc atcgcgatac ccaccagtgc gccctggatt
     7321 tgcgacattg atttttccgt cgcttctttt ggcgggagct tatcctccat catcacgcgc
     7381 tcgacgttct ccaccaccac tatcgcatca tcgacgagca gcccgatggc aagcaccatc
     7441 ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcgg cgaggatggc aaacgtccct
     7501 aacaacacca cgggtaccgc aatggtgggg atcagcgttg ctcgcatatt ctgcaagaac
     7561 agatacatca ccaggaacac cagcataatg gcttcgaaca gcgtttttac cacttcgtga
     7621 atagaaagct ggacgaatgg cgtggtgtca taagggtaga gaaccttcat tccctgcggg
     7681 aagaatggct gtaattccgc cagttttgcc ttaatggctt tcgcggtatc gagagcattc
     7741 gcgccggttg ccagcttaat ccccaggccc gccgccggtt ttccgttgat acgagcgata
     7801 acgttatagt tttcaccgcc aagttcaacc cgtgcgacat ctttcaggcg taccaccgag
     7861 ccgtcactgt ttacgcgcag ggtcactttg ccgaattctt ccggattttt taaccgcgtt
     7921 tgagcaataa tcgaggcatt caactgttgc cctggtaacg ctggcgttcc gcccaactgt
     7981 ccggcagcga tctgatcgtt ctgtaccttc aactggttaa tcacatcaac cggtgtcagt
     8041 ttatatttgt ttagcagatc ggcgtccagc cagatacgca tcgcatactg tgcgccgaaa
     8101 agctgtacgt caccgacgcc attcagacgg ctaagcgtat ctttaacgtt agaggccaca
     8161 tagtccgaga tatcgtcctg tgtggtgtct gggttatcag agacaaagcc cgccaccatc
     8221 aaatagctgc tactggactt ttcaacactg atcccctgct gctgaacctc ctgcggcagc
     8281 aacggcgtgg cgagctggag tttgttctgc acctgcactt gcgcgatatc aggatcggtc
     8341 ccggactgga aggtaagggt aattgtcacg ctaccggcgg aatcgctggt ggaggacata
     8401 tacatcaggt tatcgatacc attcatattc tgttcgataa cctgcgtcac cgtatcctgc
     8461 acggtctgcg catcagcacc tggatagttt gccgaaacag aaaccgcagg cggtgcaatg
     8521 gttggatact gagcgacggg caattgtagg atcgccagtg cgcccgccat catcagaata
     8581 atggccagca cccatgcaaa tatcggtcgt cgaataaaaa agtttgccat gtcagattac
     8641 cttacttcga tgcagtatct gcgggggtat cggtagtggc tttaacctgc tctcccggac
     8701 gcgctttttg caggccgctg acaatgactt gatcgccaga tttcagacct tcgctaatca
     8761 accatttatc gccaatcgcc tgactggcaa cgacagggcg cgcttccacc tgacttttat
     8821 cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg gctaacgcct tgttgcggga
     8881 taagaatggc gtcaggttgg acgccttcat caatccgtgc acgcacaaac atacccggca
     8941 aaagcgtatg ttgcgggtta gggaagacag cacgtagggt tatggagccg gtgctttcat
     9001 caacggtcac atcagagaat tgcagcgtac ctttcagggg ataggtttga ccgttttcca
     9061 tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa atttccttgc tctacggatt
     9121 gcttcagcct cataaaatcg ttgctggatt gggtcacatc aacgtagata ggatcgagct
     9181 gctggacagt cgccagttca gtcgtttgcc cattagtgac aagagcgcct tcggtcacgg
     9241 tcgatttgcc gatacgtccg ctaattggcg cagtcacttt ggtataagcg agattgatgc
     9301 gagcgctttc gactgtggct tttgcggcaa tcacggcggc atcggcctgg cgagcatcag
     9361 caatggcctg gtcgtactcc tgctggctga tgtatttcgt acccacgagc ggaacgtaac
     9421 gttttaccgt caaatgcgcg atggcggcgg cggcttcact tttcgccagt tcgcctttcg
     9481 cgctgtcata atttgcctga taggtcgcgg gatcgatctg gtacagggac tggcctgctt
     9541 gtacatcgct gccttcagtg aaattacgat tcagtacgat cccgctaacc tgtgggcgaa
     9601 cttcggctat acggtaagca ttggtgcggc ctggtaattc agtcttaact tctaacgggg
     9661 ccgttttcac aatatgaacg gtaacctgcg gttcaccgac gtgagctttc tcttctccct
     9721 tatcgttaca accggcgatt aaagccgcgg agatcagaat aaaggagggc aggaggaaaa
     9781 acctggcatg tttcgtcatt actattcctc aaaaaaccaa aagcgcgtta tttacccaga
     9841 aaggcaacgc gttaactcgc agaaagaaaa atacagtttg ctatcctaca aattatcatt
     9901 cgtcgatgta aggaatagtt atgaatactg gcgtctcaag gcacataaac acaaaaaaag
     9961 attaatattc tactgtttta ttttgacgcg ggttgaaaga ggcagaatta aaac
//
LOCUS       DAPHJE010000144         9857 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPHJE010000144 DAPHJE010000000
VERSION     DAPHJE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.145"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..754
                     /gene="gadW"
                     /locus_tag="RH222_003867"
     CDS             26..754
                     /gene="gadW"
                     /locus_tag="RH222_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV3798814.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1117..4230)
                     /locus_tag="RH222_003868"
     CDS             complement(1117..4230)
                     /locus_tag="RH222_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV3798815.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLIKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4255..5412)
                     /gene="mdtE"
                     /locus_tag="RH222_003869"
     CDS             complement(4255..5412)
                     /gene="mdtE"
                     /locus_tag="RH222_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV3798816.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5472..5750
                     /locus_tag="RH222_003870"
     CDS             5472..5750
                     /locus_tag="RH222_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798817.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5751..6278)
                     /gene="gadE"
                     /locus_tag="RH222_003871"
     CDS             complement(5751..6278)
                     /gene="gadE"
                     /locus_tag="RH222_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV3798818.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7077..7649)
                     /gene="hdeD"
                     /locus_tag="RH222_003872"
     CDS             complement(7077..7649)
                     /gene="hdeD"
                     /locus_tag="RH222_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV3798819.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            7904..8236
                     /gene="hdeA"
                     /locus_tag="RH222_003873"
     CDS             7904..8236
                     /gene="hdeA"
                     /locus_tag="RH222_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV3798820.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8352..8678
                     /gene="hdeB"
                     /locus_tag="RH222_003874"
     CDS             8352..8678
                     /gene="hdeB"
                     /locus_tag="RH222_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV3798821.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8742..9389
                     /locus_tag="RH222_003875"
     CDS             8742..9389
                     /locus_tag="RH222_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV3798822.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9431..>9857)
                     /locus_tag="RH222_003876"
     CDS             complement(9431..>9857)
                     /locus_tag="RH222_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3798823.1"
                     /translation="MLHQNAFVIIDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKR
                     CLGKVPVTFVPRTITIIDFVALINGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDIL
                     EKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYLV"
BASE COUNT         2682 a         2261 c         2301 g         2613 t
ORIGIN      
        1 gcagtatgaa atataaggga taatcatgac tcatgtctgc tcggtgatcc tcattcgtcg
       61 ttcattcgat atttatcatg aacagcataa aatatcgctg cataacgaga gtatcgtgct
      121 gctggagaaa aatttggcag acgattttgc gttttgttca ccggatacgc gacgactgga
      181 tatcgatgag ctgacagttt gccattactt acaaaatatt cgtcagctac cacgcaattt
      241 agggttacat agcaaagacc gtctattaat taaccagtca ccccccatgc cgctggtgac
      301 ggcgattttt gatagcttca atgaatccgg ggtaaattca ccgatactga gcaatatgct
      361 ctacctttcc tgtttatcga tgttttctca taagaaagaa ctgatcccct tacttttcaa
      421 tagcatcagc actgtttcag gaaaagttga acgccttatt agctttgata tcgccaaacg
      481 ttggtatctg cgcgatatcg cggaaagaat gtataccagc gagagtctca tcaaaaaaaa
      541 gttgcaggat gaaaatacct gtttcagtaa aatattactc gcttccagga tgtcgatggc
      601 cagacgatta ctcgagttac gtcaaattcc tctgcatact attgcagaaa aatgtggcta
      661 tagcagtacg tcgtacttta taaacacatt tcgacaatat tatggtgtaa cgccacatca
      721 gtttgcgcaa cattcgccag gtaccttttc ctgacatatt ttgcatttga atattggtca
      781 ggatctcaca cctgcttcat gtgaaactct tccctgatga tttctgccgg gctaccggct
      841 agttctcttt cgcagtttgc caggcggtat aggtattttc aggaggaaaa gactcggcat
      901 gtttgggatt attaagctga caattcatac gattacggga atgtgggtca gtattgtgct
      961 gttcaaattg atgacgaatg gctggagtgg tttctacttt cagtgctgtg tactaagcct
     1021 ggtattcctg acggttagct ggttgttgag tggtgaatgg ttagcaggaa agagtaaggc
     1081 tgaaccttca cgttcaacct tactctcatt tacacgttac gcttttttaa agcgggcaaa
     1141 gagatgttcc actacaacga aaaagaccgg aacgaagtaa attgccagca ctgttgcggc
     1201 aaacatcccg cccatcacgc cggtacctac cgcgttttgc gcgccagaac cggcaccatg
     1261 actgataacc agcggcagca cgccgagaat aaaggccaga gaggtcatca ggattgggcg
     1321 taaacgcatc cgcgccgctt cgatgattgc ctctatcggc gttttccctt ctttctgcat
     1381 catctcaacg gcaaattcga caatcaggat ggcgttcttg gcggaaagcc cgatggtggt
     1441 cagcaaacca acctggaagt agacgtcatt acttaagccg cgcagatcgg tggccagtaa
     1501 tgcgccaacg acgcctaacg gaacaaccaa catcaccgag aacggaattg accagctctc
     1561 atagagtgcg gcgagggcga ggaacaccac gaccagtgaa atggcataca gtgcaggagc
     1621 ctggtttgag gataacgctt cctgatacga cagtccggtc catgagtagc cgacgcctgc
     1681 cggaagttta gcgaccaggt ctgccataaa tttcatagcg tcaccggtac ttttcccggc
     1741 cgccgcttca cctaaaatct ccattgacgg gatgccgttg tagcgttcca atcgcggtga
     1801 accataggtc cattcggtag acgagtaggc agaaagcggt gccatcgtgc cagaggcgtt
     1861 gcgtacatac cattggttga tgttatccgg caacatacgg aacggcgtgc ctgcctggac
     1921 atacactttt ttcacccgcc cctggttgag gaagtcgttc acgtagctgc tgccgaaggc
     1981 ggtggaaatt gtctggttga tatcagacag cgccacaccc attgcctcag ctttcgcggc
     2041 gttgacgttc actttgaaca tcggcgtatc ttccaggccg ttcgggcgta ccccggtgac
     2101 ctggtcaggc gattgcgctg ccagtgataa cagctcgttt cgtgcctggg ttagtttttc
     2161 gtgccccagg ttaccgttgt ccagcagttc catatcaaaa cctgacgcgg tacccagttc
     2221 agccaccgcg ggtaagttga acgggaagac gacggcttta ttgatactgc ttaacgcaat
     2281 cattgcccgc tgaatgatcg cggtaaccga gttttcctca ccgacacgtt cagaccacgg
     2341 cttgagactg ataaacgcca ggccgttgtt ttgcccctgg ccgctgaagc caaagccgcc
     2401 aacggtaaac accgactgaa cattatcttt ctcttttata agataataat ccgtcacctg
     2461 ttgcagcact ttcgtggtgt taaccatggt ggcaccggaa ggtaactgcg cggtggtcat
     2521 aaataccccc tgatcctctt ctggtaagaa agaggtcggt gtacgcagga acagcaccgc
     2581 catcccggcg caaatcagca ggtagaccac catgtagcga ccggtacaac gcaacagcga
     2641 gcgggtgcta tcggtatagt gctgagttga tttttcaaac agcgtgttga agcgtgcgaa
     2701 cagggcgtta ggtttgtgac cgccttccgg cgcggctttc agaatggtgg cgcacagggc
     2761 aggggtcagg ctcattgcca caaatactga aagcagcatg gaggagatca gcgtgatgga
     2821 gaactggcgg tagatctccc cggttgcgcc gctcataaag gccatcggca taaacactgc
     2881 ggaaagaaca acagcaatac cgaccagcgc acgttggatc tgccccatcg atttatgcgt
     2941 cgcttccttc ggtggtagct tatcttccgc aatgacacgc tcgacgttct ccaccaccac
     3001 gatggcgtca tccaccagta gccctatcgc cagcaccatc ccgaacatag ttaacgtgtt
     3061 gatggtgaaa ccgaccgccg acaagatcgc aaacgtcccg agaataacca ccggtacggc
     3121 aatcgtcggg atgattgtgg cacggaaatt ctgcaaaaac agatacatga ccaggaagac
     3181 gaggatgata gcctcaacca gtgttttaaa aacttcctga atagaaattt cgataaacgg
     3241 cgtggtgtcg taaggataaa ccgtcttcag acttgcgggg aaataggctg ataagcggtt
     3301 cagctcctct ttgactgccc gcgaggtatc cagggcgttt gctccggtag ccagtttgat
     3361 ggcgatcccg gcagcaggtt tgccgttata gcgcgccacg gtggaataat cttccgctcc
     3421 aagttcgacg cgggcgacat cacgcagcag cacttgcgaa ccatcttgct gaactttcaa
     3481 caggattttg ccaaattctt ccggcgtttg cagacgcgtc tgcacaatga tcgaggcgtt
     3541 tagttgctgg tctgccgcct gtggcatgcc acccagttga ccaccggaaa tctggttgtt
     3601 ttgcacctta atctgggaaa taacatcgga aggtaccaga ttgtatttat tgagtttttg
     3661 cgggtccagc cagatacgca tggcgtactc ggaaccaaag agctgtacgc taccaacgcc
     3721 cgcggtacgg cttagcgggt ctttgatatt agacgctaca tagtccgcga tatcgtactg
     3781 gttgaggctg ccgttatcag aaataaacgc cgctaccatc agaatattac tgctcgactt
     3841 atcaacgcta atcccctgct gctgcaccgc ttcaggtaat gaaggcatag cgagttgcag
     3901 tttattttgc acctgaacct gtgcgatatc aggagatgtc ccagtctcga aggtcagagt
     3961 gatagaggca ttacccgccg catcactggt tgaagacatg tacatcaggc catcaagccc
     4021 attcatattt tgctcaatca cctgagtgac cgagtcttct accgtttgcg catcggcacc
     4081 tggataggta gcgctgacgg taatggtcgg tggcgcaatc tgcggatact gcgcaaccgg
     4141 taagttcatg atcgccagac cacctgcaag catcataata atggcaagta cccaggcaaa
     4201 aaccgggcga tcaataaaat agttagccat gaaagtcccc ttaagcctgc aacgttattg
     4261 tttcgattcg gtgctggcgt tttcctggct ggaggaaatt gctcgtgctt tgatacccgg
     4321 acgaatgcgt tgcaaaccgg aaacgatcac ccgatcgcca gcctgcaagc cagaggtgac
     4381 aacccactgg tcgccgatgg ctttgctggc ttcaatttcg cgtagctgca caacatcgtc
     4441 tttatccaga atgagcgccg ttgctttacc ctgggcgttg tgggtgacgc cttcctgcgg
     4501 caccagtaat acattctggc ggctaccttc atccactaat gccgtgacgt acatgccagg
     4561 cagcaagtca ccatttgggt tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc
     4621 atccaccgtc gggtcggaga atttcagcgt gccggtctgg ctgtagcgtt taccattttc
     4681 cagattgagc tgtactggcg tactgccctg aacctgtttg atttgcccac tggcgacctc
     4741 ttctttcatg cgtaagaaat cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag
     4801 acgttgtacg gtaaccagcg aatctgcctg attagcggta acgagtgcgc cgacggtcac
     4861 cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat
     4921 cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg gcttctgctt cattcaactg
     4981 ggtgcgcgcg gtgtcgtaat cctgacggct aacgtagttg gtcttcagca acgatgcctg
     5041 gcggttaaag gtgatgcggg cattgctggc ggtagagagc gctttcgcca gcgagccttt
     5101 ggcggagttt agctcggcct gtaaaggtgc aggatcaatc tgatacagcg aatcgccctg
     5161 gttcacttta tcgccttcga taaagttgcg tttaatgata ataccgccca cctggggacg
     5221 tatctcggca acttcataag gaacggttct accgggcaat tcgctcaaca cattgaccga
     5281 accgggggag agtgtgacga caccgacctc aggcgtcatg gcggcggcgt tttccgccga
     5341 tttgtcatcg caggcggtga gcatcgcgcc gcagaataac aacggtatta acagctttct
     5401 tcttctgttc attttagtcc ctgaaaattc ttgagaaata acggttcttt aaattgcgcg
     5461 cgaggagaga tatgagggat ttacagacca gcggcatagt ggggttgtca gccagtaagg
     5521 tgggatacag gtatagtgat cgacatggtg aggtcaacga caaaaaaaat gttgccgttc
     5581 tgccaacagt tcctgccagc attcgggcaa cgaagggttc tactggtgga tacacatacc
     5641 aggggaataa agtcgtttta gcctctcctt tagtaatcac cgggggtaat gaaatcagca
     5701 tctgcatccc tcgtcatgcc agccatcaat ttcagttgct tatgtcctga ctaaaaataa
     5761 gatgtgatac ccagggtgac gatgtcgctc atacgtctta gtttcagctt gtacataatg
     5821 ttgaatttct gaatctttaa tgtttgttgc gaaatattga gcgtcgacgt gatattgctt
     5881 tcatttttat ttttcagaat cagttgcata attttgaact gattatgtga aaaacataat
     5941 tgtgaattct tatggggcaa gtgtttacca taagtaatct caagaagatt tttgatactt
     6001 tctttgcggc taacaaaata aataggcgct ttagctttta gtttactgat gtgataaggt
     6061 gccaaaacaa taattctctc ggcatctaat ttctccagaa atttaatcgc ttcttcatca
     6121 aggatatgat tgtgataggt atcaatgata accttgaagg gtttattgcc tacttttttg
     6181 atctctgaca gggaattgat ttttatcatt tcgtgattat ctttcaactg ccaaaagccc
     6241 tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa cttgctcctt agccgttatc
     6301 gtttcgaaca tgtcatcctt ttcattattt acatccttgt ccgaatcgtt gttcaatata
     6361 gtaaacgcct ataagaactt tgcccttcgt gaactaatac gtctattaac actaatcaat
     6421 acttcagatt tatccatcac gcactccata tattgatcct aagtaagatt cctggttgtt
     6481 atcagtttgt aaacagtaaa caaaaagtta acgtttgcgg attactgccc aagaataagt
     6541 tatcaataac cagtttgtga tctctgaaga atattactaa agttaaaatg taatccgatt
     6601 taaatatcga gtctccttgt ttcgacttaa gctggcaatt ggattgccag ctttctttta
     6661 acgcattgaa attacctgag taaataacgc attcatgtct tcaggttttt atatagcgtc
     6721 tggagacacg gatatttatg caatgtaatc aatgccagtt gcaaaaaaat gaacccggat
     6781 tacttattta accgcccata gcatcaacgc ttacggaact ctttgttcac tttcccactg
     6841 cacttttaaa acattaaacc tgactacgga aaatatcagc catgataatg tttgaaatgg
     6901 ctaatttgcc atggagtgaa aaaaattaga tgaaattcag taggttgaaa taatcactag
     6961 caggtaatta tttcaatgat agtgcgcaat tgatctacaa cgctgcgtag cggtgagagt
     7021 attaatcgga tcatagtcac atcaagtgac tatgatccgg gtgacaaccg ggataattat
     7081 tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg cggcgctaaa tatcagttca
     7141 attcccacca gtgtggaaac cagcgttaca gacaccatcg gcgttgcacc aaggaatatc
     7201 caggcaatga cgatatccag cacaccaata acgagctgta gccagctgcc tttcattgaa
     7261 cgctgacgat accaactcat caggcgaata acccctgcaa cacagaacaa accggcaata
     7321 aatgccgcaa tggcaaaaat gcccagctcc ggtgcgcgga tgaagaaata gccgatcaat
     7381 aaataggcga ctgcgacgag gaaaccggat aataccggcc agaaattatg actgcggttg
     7441 ctgaataacc cgacaataag cgcaatgccc gagcagatta ataatgcacc cactactgtg
     7501 cttaaaatat cgccagagac gaacgggaaa ctgatacaca gcaacccgac gataaacaac
     7561 agcacggcaa taaactggat tgctctgcga tgttttttaa gcatctccag atcaaacttc
     7621 aaaattgttg ccttatctat atataacata gaaccaccct ataaaattaa gaagaaaatc
     7681 ccctgctatc aatctatgcc aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt
     7741 cctaattaca gtatctgatg catctgtaac tcattgtatt gaaataaaaa tatctgattt
     7801 tgatattttc tatcaacatg acatatacag aaaaccaggt tataacctca gtgtcgaaat
     7861 tgattcgtga cggctctttc actttatagt tgaggatatt acgatgaaaa aagtattagg
     7921 cgttattctt ggtggtctgc ttcttctgcc agttgtaagc aatgcagcgg atgcgcaaaa
     7981 agcagctgat aacaaaaaac cggtcaactc ctggacctgt gaagatttcc tggctgtgga
     8041 cgaatccttc cagccaactg cagttggttt tgctgaagcg ctgaacaaca aagataaacc
     8101 agaagatgcg gttttagatg ttcagggtat tgcaaccgta accccagcta tcgttcaggc
     8161 ttgtactcag gataaacaag ccaactttaa agataaagtt aaaggcgaat gggacaaaat
     8221 taagaaagat atgtaattcc gggaatgcgt tacatcgtac ttccttgcat attgaacagg
     8281 ccggaatatc ttctttaaaa gcagctattc ctcctgttca tatataatct ctatattgaa
     8341 tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag
     8401 cggctttgtc actggtgaac gcacaatctg cgctggcagc caatgaatcc gctaaagata
     8461 tgacctgcca ggaatttatt gatctgaatc caaaagcaat gaccccggtt gcatggtgga
     8521 tgctgcatga agaaacagta tataaaggtg gcgataccgt tactttaaat gaaaccgatc
     8581 tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa cccgcagaaa aatttgtata
     8641 ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg aggtacaagt aaaaaggagt
     8701 agcaagttga gccatcttgc tgctcctttt tgcattttta tatgacagca gaatttatta
     8761 tacgtcttat actcgcggca attgcctgtg gcgctattgg catggaaagg caaatgcgcg
     8821 gcaaaggagc agggttacgc acacatgtat taattggcat gggaagcgcc ctgtttatga
     8881 ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca
     8941 gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat cggtgcaggt aacattctcg
     9001 ttcgtaacca aaatattgta ggtctgacga cggcagcgga tatctgggtg accgccgcca
     9061 taggtatggt tattggcagc ggtatgtacg aactgggtat ttatggttca gtgatgacct
     9121 tgttggtgct ggaagtcttc catcaattaa ccttccgcct gatgaacaaa aattaccatt
     9181 tacagctgac attagtgaac ggtaatacgg tatcaatgct cgactggttt aagcagcaaa
     9241 aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg
     9301 atatccagct tcatgccaca acgtcgatag aagacctgtt gcgattatta aaaggtatgg
     9361 caggggtgaa aggcgtttca attagctaat ctgtgtggca gcgtagccag actcaccgta
     9421 agcctgaaat tcacaccaga taatcaatat gctgatggcg taccagctca ttgatccgct
     9481 taagattgag gatcatcatg atattgtgtt tatggcagta gaacgttttt agcgatattt
     9541 tcagtttctc cagaatatct tccgttgtca tttgattcgc aatgcagctc aaaacctgat
     9601 gttgcttgcg cgaaagtgac acattcgctt caggcacaga gcagtaactg ccattgatga
     9661 gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa ggtcaccggt actttcccca
     9721 gacagcgttt aatattaaag ggggagaaaa taatcacatg gacaggtttt aatcgttcaa
     9781 tttgagtgag aaaattagaa tgaaatacat tattcattaa tgtatcaata atgacgaagg
     9841 cattctgatg caacatt
//
LOCUS       DAPHJE010000145         9792 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPHJE010000145 DAPHJE010000000
VERSION     DAPHJE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.146"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            233..754
                     /gene="fecI"
                     /locus_tag="RH222_003877"
     CDS             233..754
                     /gene="fecI"
                     /locus_tag="RH222_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HDV3798824.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            751..1704
                     /gene="fecR"
                     /locus_tag="RH222_003878"
     CDS             751..1704
                     /gene="fecR"
                     /locus_tag="RH222_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HDV3798825.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1791..4115
                     /gene="fecA"
                     /locus_tag="RH222_003879"
     CDS             1791..4115
                     /gene="fecA"
                     /locus_tag="RH222_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HDV3798826.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4160..5062
                     /gene="fecB"
                     /locus_tag="RH222_003880"
     CDS             4160..5062
                     /gene="fecB"
                     /locus_tag="RH222_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HDV3798827.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5059..6057
                     /gene="fecC"
                     /locus_tag="RH222_003881"
     CDS             5059..6057
                     /gene="fecC"
                     /locus_tag="RH222_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HDV3798828.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6054..7010
                     /gene="fecD"
                     /locus_tag="RH222_003882"
     CDS             6054..7010
                     /gene="fecD"
                     /locus_tag="RH222_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HDV3798829.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7011..7778
                     /gene="fecE"
                     /locus_tag="RH222_003883"
     CDS             7011..7778
                     /gene="fecE"
                     /locus_tag="RH222_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HDV3798830.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8335..8592)
                     /locus_tag="RH222_003884"
     CDS             complement(8335..8592)
                     /locus_tag="RH222_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798831.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8675..9459)
                     /locus_tag="RH222_003885"
                     /pseudo
     CDS             complement(8675..9459)
                     /locus_tag="RH222_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9526..>9792
                     /locus_tag="RH222_003886"
     CDS             9526..>9792
                     /locus_tag="RH222_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3798832.1"
                     /translation="MSRKTQRYSKKFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKKATV"
BASE COUNT         2144 a         2789 c         2686 g         2173 t
ORIGIN      
        1 tccccatcct ttagggcagt gaggatgtca ataatagcag accacatcat gagtcattct
       61 gaccgacaca tgccagaagc agaggctcca tcccatttta tacctacctt ataacactta
      121 gaaaaacaac atgttaaaaa tgtctattgg aaacaatttt atttccaatt gtaatgataa
      181 ccattctcat attaatatga ctacgtgata attaactttt gatgcactcc gcatgtctga
      241 ccgcgccact accacagctt ccttaacgtt cgagtcgctt tatggcacac atcacggctg
      301 gttgaaaagc tggctgacgc gcaaactcca gtctgctttt gatgcagatg acattgccca
      361 ggacactttt ttgcgggtaa tggtcagcga aacgctctcg acgatccgcg atcctcgctc
      421 cttcctctgc actatcgcca aacgcgtgat ggtggacctg tttcgccgaa acgcgctgga
      481 aaaagcgtat ctggagatgc tggcgcttat gccggagggg ggagcgcctt cacctgagga
      541 acgcgaaagc caactcgaga ccctacaact cctcgacagc atgctggacg ggctaaacgg
      601 caaaacacgt gaagcgtttc tgctttcgca actggatggt ctgacataca gcgagattgc
      661 gcacaaactc ggtgtttcca tcagctccgt gaaaaaatac gtggcgaaag ccgtcgagca
      721 ctgcctgctg ttccgtctgg agtatgggtt atgaatcctt tgttaaccga ttcccgccgt
      781 caggcgctgc gttcagcttc ccactggtat gccgtgctaa gcggcgagcg cgtcagccca
      841 caacaggaag cgcgctggca acagtggtat gaacaggatc aggataacca gtgggcctgg
      901 cagcaggttg aaaacctgcg caaccagctt ggcggtgtgc ctggcgacgt tgccagccgg
      961 gcgttgcacg atacccgcct cacccgccgt cacgtgatga aaggattact gctgttgctc
     1021 ggcgctggcg gaggctggca gctctggcag tcggaaaccg gcgaaggtct gcgggcagat
     1081 taccgcaccg ccaaaggcac agtcagccgt cagcaactgg aagatggctc cctgctcacg
     1141 ctgaataccc aaagcgcggc ggatgtgcgt tttgatgcgc atcagcgcac cgtccggctc
     1201 tggtacggtg aaatcgccat tactaccgcg aaagatgccc tgcaacgccc cttccgtgtc
     1261 ctgacccgtc agggccagct cactgcttta gggacagaat ttaccgtccg ccagcaggat
     1321 aatttcacgc agcttgacgt tcagcagcac gctgtggaag tgcttctcgc cagtgccccc
     1381 gcgcaaaaac gcatcgtgaa cgctggtgaa agcctgcagt tcagcgcctc tgagtttggc
     1441 gcagtgaaac cgctggatga cgagagtaca agctggacga aggacatcct gagcttcagc
     1501 gataaaccac tgggtgaggt gatagccacg ctaacccgtt accgcaacgg cgtgctgcgc
     1561 tgcgatcccg ccgttgccgg gctgcgcctg agcgggacgt tcccgctgaa aaataccgat
     1621 gcgatcctga acgttatcgc gcaaacgctt cccgttaaaa ttcagtctat tacgcggtac
     1681 tggataaaca tttcaccact gtaaggaaaa taattcttat ttcgattgtc ctttttaccc
     1741 ttctcgttcg actcatagct gaacacaaca aaaatgatga tggggaaggt atgacgccgt
     1801 tacgcgtttt tcgtaaaaca acacctttgg ttaacgccat tcgcctgagc ctgctgccgc
     1861 tggccggtct ctcgttttcc gcttttgctg cacaggttaa tatcgcaccg ggatcgctcg
     1921 ataaagcgct caatcagtat gccgcacaca gcggatttac cctctcggtt gacgccagcc
     1981 tgacgcgcgg caagcagagc aacggcctgc acggcgatta cgacgtcgag agcggcctgc
     2041 aacaactgct ggacggcagc ggactgcagg taaaaccgct gggaaataat agctggacgc
     2101 tggagcccgc gcccgcacca aaagaagatg ccctgaccgt ggtcggcgac tggctgggtg
     2161 atgcgcgtga aaacgacgta tttgaacatg ctggcgcgcg tgacgtgatc cgccgtgagg
     2221 atttcgccaa aaccggcgca accaccatgc gtgaggtact taaccgcatc cctggcgtca
     2281 gcgcgccgga aaacaacggc accggcagcc acgacctggc gatgaacttt ggcatccggg
     2341 gcctgaaccc gcgcctcacc agccgctcga ccgtcctgat ggacggcatc cccgtcccct
     2401 tcgcccctta cggtcagccg cagctttcac tggctcccgt ttcgctcggc aacatggatg
     2461 ccattgacgt ggtacgcggt ggtggtgcgg tgcgttacgg accgcagagc gtgggcggcg
     2521 tggtgaactt tgttacccgt gccattccgc aggactttgg tatcgaggcg ggcgtggaag
     2581 gtcagctcag cccaacctct tcacaaaaca acccgaaaga gacgcacaac ctgatggtgg
     2641 gcggcacagc ggacaacggt tttggcaccg cgctgctcta ctccggcacg cgcggcagtg
     2701 actggcgcga gcacagcgcc acccgcatcg acgacctgat gctgaaaagc aaatatgcgc
     2761 cggatgaggt gcacaccttc aacagcctgc tgcaatatta cgacggtgaa gccgacatgc
     2821 ccggtggcct gtctcgcgcg gattacgacg ccgatcgctg gcaatccacc cgcccgtatg
     2881 accgcttctg gggtcgtcgc aagctggcga gcctgggcta ccagttccag ccagacagcc
     2941 agcataaatt caacattcag gggttctaca cccaaaccct gcgcagcggc tacctggagc
     3001 aaggcaaacg catcaccctc tcgccgcgta actactgggt gcgcggtatt gagccacgct
     3061 acagccagat ctttatgatc ggcccttccg cgcacgaagt gggcgtgggc tatcgctatg
     3121 tgaatgaatc aacgcatgaa atgcgttact acaccgccac cagcagcggg cagttgccgt
     3181 ccggctcaag cccttacgac cgcgatacgc gttccggcac cgaggcgcac gcctggtatc
     3241 tggatgacaa aatcgacatc ggcaactgga ccatcacgcc gggtatgcgt ttcgaacata
     3301 tcgagtcata ccagaacaac gccatcacag gcacgcacga agaagtgagc tataacgcac
     3361 cgcttccggc gttgaacgtg ctctatcacc tgactgacag ctggaatctt tatgcaaaca
     3421 ctgaaggctc gttcggcacc gtacagtaca gccagattgg caaggctgtg caaagcggca
     3481 atgtggaacc ggaaaaagcg cgaacctggg aactcggtac ccgctacgac gacggcgcgc
     3541 tgacggcgga aatggggctg ttcctgatta actttaacaa tcagtacgac tccaaccaga
     3601 ccaacgacac cgtcactgca cgtggcaaaa cgcgccatac cgggctggaa acgcaggcac
     3661 gttacgatct gggtacgcta acgccaacgc ttgataacgt ttccatctac gccagctatg
     3721 cgtatgtgaa cgcggaaatc cgcgagaaag gcgacaccta cggcaatctg gtaccattct
     3781 ccccgaaaca taaaggcacg ctgggcgtgg actacaagcc aggaaactgg acgttcaatc
     3841 tgaacagcga tttccagtcc agccagtttg cggataacgc caatacggtg aaagagagcg
     3901 ccgacggcag taccggccgc attcccggct tcatgctctg gggcgcacgc gtggcgtatg
     3961 actttggccc gcagatggca gatctgaacc tggcattcgg tgtgaaaaac atcttcgacc
     4021 aggactactt catccgctct tatgacgaca acaacaaagg catctatgca ggccagccgc
     4081 gcacgctgta tatgcagggg tcgttgaagt tctgatgtgt taacgcccgg cttgccgggc
     4141 ttttagctgg aatgtgatta tgttggcatt tatccgtttt gtttttgcag gcctgctgct
     4201 ggtgatcagc cacgcctttg ccgccacggt tcaggacgaa cacggcacgt ttacactcga
     4261 aaaaacgcca caacggattg tggtgctgga actctcgttc gccgatgcgc tggccgccgt
     4321 ggacgtcagc ccgatcggta ttgccgacga taacgatgca aaacgcatcc tgcccgaagt
     4381 gcgtgcgcac ctgaaaccgt ggcagtccgt cggaacgcgc gcgcagccga gcctggaagc
     4441 cattgccgct ctgaaaccag acctgatcat tgccgacagc agtcgccatg cgggggttta
     4501 catcgccttg cagcaaatcg cgccggtact gctgcttaag tcccgcaacg aaacctacgc
     4561 tgaaaatttg caatctgcgg ctatcatcgg cgaaatggtg ggtaaaaagc gagagatgca
     4621 ggcacgcctg gaacaacata aagagaggat ggcgcagtgg gccagccagc ttcccaaagg
     4681 gacacgcgtg gcctttggca catcacgcga acagcaattc aacctgcata ctcaggagac
     4741 ctggaccggc agcgtgctgg cctctctggg gctgaacgtt cccgctgcga tggcgggtgc
     4801 gtccatgccg tccatcggcc tggagcaact gctggcggtc aatcctgcct ggctgctggt
     4861 tgcccactat cgcgaagaga gcattgttaa acgctggcaa caagatccgc tctggcagat
     4921 gttaaccgcc gcgcagaagc agcaggttgc ttcggtcgac agtaacacct gggcgcggat
     4981 gcgcggtatt tttgctgcag agcgtattgc cgctgacacg gtaaaaatct tccaccatca
     5041 gccgcttacc gttgtgaaat gaccgcgata aaacacccgg tgctgctgtg ggggcttccc
     5101 gttgcagcac ttattattat tttctggctg agtctgtttt gctactcggc cattcctgtt
     5161 tccggagcag atgcaacccg cgccctgctg cctggacaca cgccaacgct accagaagcg
     5221 ctggtgcaaa accttcgttt gccacgaagc ctggtcgccg ttctgatcgg cgcaagcctg
     5281 gcgctcgcgg gcacgctgct gcaaaccctg acccacaacc caatggcctc tccttcactg
     5341 ctcggcatta acagcggcgc ggcgctggct atggcgctta ccagcgcgct gagtccgacg
     5401 ccgattgcag gctattctct gtcgttcatc gcggcatgcg ggggcggcgt gagctggctg
     5461 ctggtcatga ccgcaggagg cggatttcgt catacccatg acagaaacaa actgatcctc
     5521 gcgggtatcg cgctgtcggc cttttgtatg ggccttaccc gcatcaccct gctgctggcc
     5581 gaagatcatg cttacggcat cttttactgg ctggcaggcg gagtgtccca cgcccgctgg
     5641 caggatgtct ggcagctctt gccggtggtg gtcactgcag tccctgtcgt gttgctgctg
     5701 gcgaatcaac tgaacctgct caacctcagc gacagcaccg cccatacgct gggagtgaac
     5761 ctgacgaggc tacgtttggt catcaatatg ttagtgctgc ttctggttgg tgcgtgcgtc
     5821 agcgtggcag gtccggtggc gtttatcggt ctgctggtgc cacatctggc gcgcttctgg
     5881 gcaggcttcg atcagcgcaa cgtactgcca gtgagcatgc tgctgggggc cacgctgatg
     5941 ctgctggcag atgtactcgc acgcgcactg gccttccccg gagatctgcc cgcaggcgca
     6001 gtgctggcgc tgattggcag cccttgcttt gtctggcttg tgaggaggcg aggatgaaaa
     6061 ttgcgctggt tattttcatc acccttgccc tggcgggctg tgcgctgtta tcactccata
     6121 tgggagtgat ccccgtgccg tggcgcgcgc tgctgaccga ctggcaggcc ggacgcgagc
     6181 attattatgt attgatggag taccgactgc cgcgcttgct gctggcactg tttgtcggtg
     6241 cagccctcgc cgtggcgggc gtgctgatac aggggattgt gcgcaaccct ctggcatcac
     6301 cggatattct cggtgttaac catgccgcca gcctggcctc tgtgggggct ctacttctta
     6361 tgccgtcact gcccgtgatg gtgctgccgc tgctggcctt tgcgggcggc atggcggggt
     6421 tgatattgct gaagatgctg gcaaagaccc accagccgat gaagctggcg ctcaccggcg
     6481 tggcgctttc tgcatgctgg gccagcctga cggattatct gatgctctcg cgcccgcagg
     6541 atgtgaacaa cgccctgctg tggctgaccg gcagcttatg gggccgtgac tggagctttg
     6601 tgaagattgc catcccgctg atgattttat ttctgccgct gagcctgagt ttttgccgcg
     6661 atctcgacct ccttgcactc ggcgatgcgc gcgccaccac gctcggtgtg tcggtgcccc
     6721 atacccgatt ctgggctttg ttactagctg tcgccatgac atctaccggc gtggccgcct
     6781 gcggcccgat tagctttatt ggtctcgtgg tgccgcatat gatgcgtagc atcaccggtg
     6841 gacgtcaccg cagactgctg cctgtttcgg ccctgacagg tgcgttgctg ttggtggttg
     6901 ccgatctgct ggcgagaatt attcatcccc cactggagct cccggttggc gtgctgaccg
     6961 ccattatcgg tgcgccgtgg tttgtctggt tgcttgtgag aatgcgataa atgactttac
     7021 gaactgaaaa tctgacggtc agttacggga cagacaaggt acttaacgac gtttcactct
     7081 cactgccaac ggggaagatc accgccctga tcggtcctaa cggttgcggg aaatcgacgc
     7141 tgttaaactg tttttcgcgg cttttaatgc cgcagtctgg caccgtattt ctcggcgata
     7201 atcccataaa tatgctctca tcgcgccagt tggcccgcag gctttcgctg ctgcctcagc
     7261 accatttaac gccagagggg atcacagtcc aggagctggt ttcgtatggt cgtaatccct
     7321 ggctgtcact ctgggggcgt ctctccgctg aagacaatgc acgagttaat gtcgccatga
     7381 accagacccg gatcaatcat cttgccgttc gtcggttaac cgagctttcc ggcggtcagc
     7441 gccagcgcgc atttctggca atggtcctgg cccagaatac gcccgttgta ttacttgatg
     7501 agccaaccac ctatcttgat atcaatcacc aggtggacct gatgcggttg atgggcgaac
     7561 tccggactca ggggaaaacg gtggtcgctg tgctgcacga ccttaatcag gctagccggt
     7621 actgcgatca actggtggta atggcaaacg gacatgttat ggcgcaaggc acaccagaag
     7681 aggtgatgac cccaggattg ctgagaacag tattcagcgt ggaagcggaa atacaccccg
     7741 agccggtatc tggcaggccg atgtgcctaa tgaggtagat tgcacaggcc gtaagaacca
     7801 aaccacgact gaatgaaact ggactggcgc cagcaagcct gttcagactg gggctgaact
     7861 tttccggact ctgaaagatt accaatactc atcgtccatc cgcttgcttt aggctgacag
     7921 gttcataatc aacgcaaacc agagctgtac aggcttgggc gcggctttca aaccagtcgt
     7981 gatcacggca atcaattttg aactctgctt aacggacatt tctgtataac ccttacggca
     8041 acgaaaaacg cgaagttaaa attttagaaa cccaaaaacg tgacatgact aagtttagat
     8101 ttcagggggg agatcaaaaa atttcgctct gtgccagagc ggacattcac ggagctggtt
     8161 cattaccaat gaggttgggc ttttgaggat aaatcaatga tcagacgcca acgtaaatca
     8221 aaagcaaccc tggaacggaa ttgctaatcc agtttctgac catcgatttt tctaaaaagt
     8281 gtgcgtttgc tactacttag gtaggtgcag ctttcttaat caccggcagc cacgttatac
     8341 aggccagttg atggatcgat tgttatcaat gatatcttca tgagtcggtg tctcacccag
     8401 cttaccgaag ctggcataaa gtgaaggcag acgggcccgt ccttctccct ttttcgccag
     8461 agggaaagcg cgaagcatgg tggcagcctc ccagtcacaa caataggatg gtgtgcacgg
     8521 ctgctgacgc catgattcag cgatagagcc ggaaaatacg gggtcaaagc cggtatcatt
     8581 aaccagagtc atcgtgacct gtactgcggc tggatgatct cctgctactg caactgccag
     8641 gcgaccgcgg ctcccttcag gtagtctgtt gtggtcaact aaaactggcc tccgcgttag
     8701 agtttttcca gtatcggttt tctgattcgt ttggtggtaa cccaccatta tattcgtgcg
     8761 gtcttagtgc gctgtaatat ccaacgatat agtccgttat tgcgtgagct gcatcgctga
     8821 agcttacata gcccgtcgct ggcacccatt cgttcttcag actcctgaag aagcgctcca
     8881 ttgggctgtt atcccagcag tttccacgcc gactcatact ctgcctgatc cggtatcgcc
     8941 acagtaactg ccggaactgc ctgctcgtat aatgactgcc ttgatcgctt ggaacatcac
     9001 cccgacgggc ttaccacggg tttcccatgc catttccagt gctttcatgg taagcctgct
     9061 gtccggcgag aacgacatgg cccagcccac tggttttctt gcgaacaggt cgagaacaac
     9121 ggcgaggtac gcccagcgct tacccgtcca gatataggtc acatcaccgc accacacctg
     9181 atttggctcg gtcacggcga actgcctttc aaggtagtta gggatagcaa catgttcatg
     9241 accaccacgt ttataccggt gagtcggctg ctgacagctg accagcccca gctctttcat
     9301 gagcctgcca gcaagccagc gtcccatctg gtagcctctc cgggttgcca ttgtggcgat
     9361 gcttcttgct ccggccgaac cgtggctgat gccatgtagc tcaagtacct gactgcgtaa
     9421 tacagcccgt ctgccgtctg gtttttcagg acagtttttc ctgaggtagc ctgagtttaa
     9481 cggacactcc ttcctgaaat agaatggcat cagaaggagc taataatgag cagaaaaacc
     9541 caacgttact ctaaaaagtt caaagccgaa gctgtcagaa cggttcttga aaatcaactt
     9601 tcgatcagtg aaggcgcttc ccgattatct cttcctgaag gcactttagg acaatgggtt
     9661 accgccgcca gaaaagggct cggtactcct ggttcccgca cggtggctga actggaatct
     9721 gaaattctgc aactgcgtaa ggcgttaaat gaagctcgcc ttgagcgaga tatattaaaa
     9781 aaagcaactg ta
//
LOCUS       DAPHJE010000146         9783 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPHJE010000146 DAPHJE010000000
VERSION     DAPHJE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.147"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            198..1175
                     /gene="csiD"
                     /locus_tag="RH222_003887"
     CDS             198..1175
                     /gene="csiD"
                     /locus_tag="RH222_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV3798833.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1195..2463
                     /gene="lhgO"
                     /locus_tag="RH222_003888"
     CDS             1195..2463
                     /gene="lhgO"
                     /locus_tag="RH222_003888"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV3798834.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2486..3934
                     /gene="gabD"
                     /locus_tag="RH222_003889"
     CDS             2486..3934
                     /gene="gabD"
                     /locus_tag="RH222_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3798835.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3948..5228
                     /gene="gabT"
                     /locus_tag="RH222_003890"
     CDS             3948..5228
                     /gene="gabT"
                     /locus_tag="RH222_003890"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV3798836.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5466..6866
                     /gene="gabP"
                     /locus_tag="RH222_003891"
     CDS             5466..6866
                     /gene="gabP"
                     /locus_tag="RH222_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV3798837.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6887..7549
                     /gene="csiR"
                     /locus_tag="RH222_003892"
     CDS             6887..7549
                     /gene="csiR"
                     /locus_tag="RH222_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV3798838.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7550..7999)
                     /gene="kbp"
                     /locus_tag="RH222_003893"
     CDS             complement(7550..7999)
                     /gene="kbp"
                     /locus_tag="RH222_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV3798839.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8083..8241)
                     /locus_tag="RH222_003894"
     CDS             complement(8083..8241)
                     /locus_tag="RH222_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV3798840.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8424..8723
                     /locus_tag="RH222_003895"
     CDS             8424..8723
                     /locus_tag="RH222_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3798841.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8733..9257
                     /gene="ygaP"
                     /locus_tag="RH222_003896"
     CDS             8733..9257
                     /gene="ygaP"
                     /locus_tag="RH222_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV3798842.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9304..9708)
                     /gene="stpA"
                     /locus_tag="RH222_003897"
     CDS             complement(9304..9708)
                     /gene="stpA"
                     /locus_tag="RH222_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV3798843.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2237 a         2520 c         2683 g         2343 t
ORIGIN      
        1 gctttagtta ttcatttcct gtctcacttt gccttaatac cctacgttaa atgttactaa
       61 tttattgctt ttgatcacaa taagaaaaca atatgtcgct tttgtgcgca tttttcagaa
      121 atgtagatat ttttagatta tggctacgaa atgagcatcg ccatgtcacc ctacatctca
      181 taagaggatc gcttctgatg aatgcactga ccgccgtaca aaataacgct gtcgattcag
      241 gccaggacta tagcggattc actctcatcc cgtcggcgca atccccgcgt ctgctggaac
      301 tcaccttcac cgaacagacg accaaacagt ttctcgaaca ggttgccgaa tggcccgtgc
      361 aggcgctgga gtacaaatcg tttctgcgtt ttcgggtagg caaaattctt gacgatctgt
      421 gtgcgaatca gctgcaacca ttgctgttga agaccctgct aaaccgcgct gaaggtgcgc
      481 tgttgatcaa tgcggtgggt atcgatgatg tggcgcaggc agatgagatg gtgaagctgg
      541 caacggcggt agcgcatctg attggtcgct caaatttcga tgcgatgagc ggtcagtatt
      601 acgcgcgttt cgtggtgaaa aatgtcgata actcagacag ctatctgcgt cagccgcacc
      661 gcgtgatgga actgcacaac gacggcacgt atgtcgaaga gatcaccgat tacgtgctga
      721 tgatgaaaat cgatgagcaa aacatgcagg gtggaaattc gttgctgctg catctcgatg
      781 actgggaaca tctggaccac tatttccgcc atccactggc gcgtcgcccg atgcgctttg
      841 ccgcgccgcc gagcaaaaac gtcagcaaag atgttttcca tccagtgttc gacgttgatc
      901 aacagggtcg cccggtgatg cgctatatcg accagttcgt ccagccaaaa gattttgaag
      961 aaggcgtgtg gttgagcgag ctttccgatg ccattgaaac cagcaaaggc attctttctg
     1021 tacccgttcc cgttggcaaa ttcctgttga taaacaacct gttctggctg cacggtcgcg
     1081 accgctttac tccgcacccg gatctgcgcc gtgaactgat gcgtcagcgt ggctatttcg
     1141 cttacgccac tcaccactac cagacgcatc agtaagcgca aaggaattga gcggatgtat
     1201 gattttgtga ttattggcgg cggcatcatc ggcatgtcga ccgccatgca actgattgat
     1261 gtttatccgg atgcccgcat tgcgttgctg gaaaaagagt ccggcccggc ctgtcaccag
     1321 acgggccaca acagcggcgt gatccatgcc ggggtttatt acacgcccgg tagcctgaag
     1381 gcgcagtttt gcctggcggg aaaccgcgcc accaaagcct tttgcgatca aaacggcatt
     1441 cgctatgaca actgcggcaa gatgctggtc gccacctctg aactcgaaat ggaacggatg
     1501 cgcgcgttgt gggaacgcac ggcggcaaac ggtatcgagc gcgagtggtt aaacgccatg
     1561 gaactgcgcg agcgcgaacc gaatatcacc gggcttggcg gcatttttgt gccatccagc
     1621 ggcattgtca gctaccgcaa cgtgacggcg gcaatggcaa aaatcttcca ggccagaggc
     1681 ggtgagatta tctataacgc cgaagtcagc ggcctcagtg agcataaaaa cggcgtggtg
     1741 atacgtaccc gccagggcgg cgaatatgaa gcatcgacgc tgattagctg ttccgggctg
     1801 atggctgacc ggctggtgaa aatgctcggc ctcgaaccgg gctttattat ctgcccgttc
     1861 cgcggcgagt atttccgcct tgcgccggag cataaccaga ttgttaacca tctgatttac
     1921 cctattcccg acccggcaat gccgtttttg ggcgtgcatc tcacccgaat gatcgatggc
     1981 agcgtgaccg tcgggccaaa cgcggtgctg gcttttaaac gcgaaggcta ccgcaagcgc
     2041 gacttctcgt ttagcgacac gctggaaatt ttaggctcgt cggggattcg ccgggtgctg
     2101 caaaaccatc tacgctcagg actgggcgag atgaaaaact cgctgtgcaa aagcggctat
     2161 ctgcggctgg tgcaaaagta ttgtccccgg ctttcgttaa gcgatctcca gccctggccc
     2221 gccggtgtgc gggcgcaggc ggtatcgccg gatggcaagt tgattgacga ttttctgttt
     2281 gtcaccaccc cgcgcacgat ccacacctgc aatgcgccct ccccggcagc gacatcagca
     2341 attcctattg gtgcgcatat tgtcagcaag gtacaaacgc tgttggcaag ccagagtaac
     2401 cccggacgca cgctgcgagc ggcacgtagt gtggatgcct tacacgccgc atttaatcaa
     2461 taacctttga aaacaggacg taacaatgaa acttaacgac agtaacttat tccgccagca
     2521 ggcgttgatt aacggggaat ggctggacgc caacaatggt gaagtcatcg acgtcaccaa
     2581 tccggcgaac ggcgacaagc tgggtagcgt gccgaaaatg ggcgctgatg aaacccgcgc
     2641 cgctatcgac gccgccaacc gcgccctgcc cgcctggcgt gcgctcaccg ccaaagaacg
     2701 cgccaacatt ctgcgcaact ggttcaattt gatgatggag catcaggacg atttagcgcg
     2761 cctgatgacc ctagaacagg gcaaaccgct ggctgaagcg aaaggcgaaa ttagctacgc
     2821 cgcctccttt attgagtggt ttgctgaaga aggcaaacgc atttatggcg acaccattcc
     2881 cggtcatcag gccgataagc gcctgattgt tatcaagcag ccgattggcg ttaccgcagc
     2941 tatcacgccg tggaacttcc cggcggcgat gattacccgc aaagccggtc cggcgctggc
     3001 ggcaggctgc accatggtgc tgaaacccgc cagtcagacg ccgttctctg cgctggcgct
     3061 ggcggagctg gcgattcgcg cgggcattcc ggctggggta tttaacgtgg tcaccggttc
     3121 ggcgggcgca gtcggtaacg aactgaccag caacccgctg gtgcgcaaac tgtcgtttac
     3181 cggttcgacc gaaattggcc gccagttaat ggaacagtgc gcgaaagaca tcaaaaaagt
     3241 gtcgctggag ctgggcggca acgcgccgtt tatcgtcttt gacgatgccg atctcgataa
     3301 agccgtggaa ggtgcgctgt cctcgaaatt ccgcaacgcc gggcaaacct gcgtctgcgc
     3361 caaccgttta tacgtgcagg acggcgtgta tgaccgcttt gccgaaaaat tacagcaggc
     3421 ggtgagcaaa ctgcacatcg gcgacgggct ggagaatggc gtcaccatcg ggccgctgat
     3481 cgatgaaaaa gcggtagcaa aagtggaaga gcatattgcc gatgcgctgg agaaaggcgc
     3541 gcgcgtggtt tgcggaggta aagcgcacga acgcggcggc aacttcttcc agccaaccat
     3601 tctggtggac gttccggcca acgccaaagt gtcgaaagaa gagacgttcg gccccctcgc
     3661 cccgctgttc cgctttaaag atgaagccga tgtgattgcg caagccaatg acaccgagtt
     3721 tggccttgcc gcctatttct acgcccgtga tttaagccgc gtcttccgcg tgggcgaagc
     3781 gctggagtac ggcatcgtcg gcatcaatac cggcattatt tccaatgaag tggccccgtt
     3841 cggcggcatc aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca tcgaagatta
     3901 cttagaaatc aaatatatgt gcatcggtct ttaactggag aacgcgaatg agcagcaata
     3961 aagagttaat gcagcgccgc agtcaggcga ttccccgtgg cgttgggcaa attcacccca
     4021 ttttcgctga ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt gagtatcttg
     4081 atttcgcggg cgggattgcg gtgctcaata ccgggcatct gcatccgaaa gtggtggctg
     4141 cggtggaagc gcagttgaaa aaactgtcgc acacctgctt ccaggtgctg gcctacgagc
     4201 cgtatctgga gctgtgcgag attatgaatc agaaggtgcc aggcaatttt gccaagaaaa
     4261 cgctgctggt caccacaggt tctgaagcgg tggaaaacgc ggtgaaaatc gcccgcgccg
     4321 ccaccaaacg tagcggcacc atcgctttta gcggcgcgta tcacgggcgc acgcattaca
     4381 cgctggcgct gaccggcaag gtgaatccgt actctgcggg catggggctg atgccgggtc
     4441 atgtttatcg cgcgctttat ccttgcccgc tgcacggcat aagcgaggat gacgctatcg
     4501 ccagcatcca ccggatcttc aaaaatgatg ccgcgccgga agatatcgcc gccatcgtga
     4561 ttgagccggt tcagggcgaa ggcggtttct acgccgcgtc gccagccttt atgcagcgtt
     4621 tacgcgctct gtgtgacgag cacgggatca tgctgattgc cgatgaagtg cagagcggcg
     4681 cggggcgtac cggcacgctg tttgcgatgg agcagatggg cgttgcgccg gatcttacca
     4741 cctttgcgaa atcgatcgcg ggcggcttcc cgctggcggg cgtcaccggg cgcgcggaag
     4801 tgatggatgc cgtcgctcca ggcggtctgg gcggcaccta tgcgggtaac ccgattgcct
     4861 gcgtggctgc gctggaagtg ttgaaggtgt ttgagcagga aaatctgctg caaaaagcca
     4921 acgatctggg gcagaagttg aaagacggat tgctggcgat agccgaaaaa cacccggaga
     4981 tcggcgacgt acgcgggctg ggggcgatga tcgccattga gctgtttgaa gacggcgatc
     5041 acaacaagcc ggacgccaaa ctcaccgccg agatcgtggc tcgcgcccgc gataaaggcc
     5101 tgattcttct ctcctgcggc ccgtattaca acgtgctgcg catccttgta ccgctcacca
     5161 ttgaagacgc tcagatccgt cagggtctgg agatcatcag ccagtgtttt gatgaggcga
     5221 agcagtagcg ccgctcctat gccggaggcg acgctgcgcg tcttgtccgg cctacgggga
     5281 tccaggtcgg ataaggcgtt tacgccgcat ccgacaatct gtacgtgaac aggaagaaat
     5341 ctatgttggc cgggtaaggc ggagccgctc tccggcaaaa agaatcaata acaattatac
     5401 gcgtgacccg acgcgggaaa tgtcggggcg ctctcccaag tgacacactt tcgagaggat
     5461 tcaggatggg gcaatcatcg caaccacatg agttaggcgg cgggctgaag tcacgccacg
     5521 tcaccatgtt gtctattgcc ggtgttatcg gcgcaagtct gtttgttggt tccagcgtcg
     5581 ccatcgccga agcgggtccg gcggtattac tggcctatct gttcgccggg ctactggtgg
     5641 ttatgattat gcggatgttg gcggaaatgg cggttgccac gcccgatacc ggttcgtttt
     5701 ccacctatgc cgataaagct attggtcgtt gggcgggtta taccatcggc tggttgtact
     5761 ggtggttttg ggtactggtt atcccgctgg aagccaacat cgccgccatg atcctgcact
     5821 catgggttcc aggcattccc atctggttat tttccctcgt cattaccctc gccttaactg
     5881 gcagtaactt attaagcgtt aaaaactacg gcgaatttga gttctggctg gcgctgtgca
     5941 aagtcatcgc tatcctggcc tttattttcc ttggtgcagt cgcaattagc ggtttttacc
     6001 cttatgccga agtgagcggg atctcaagat tgtgggatag cggcggcttt atgcccaacg
     6061 gtttcggtgc ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg ggcgcagaaa
     6121 ttgtcaccat tgccgccgcg gaatccgaca cgccggaaaa acatattgtc cgcgccacca
     6181 actcggttat ctggcgtatt tctatcttct atttatgttc tatttttgtc gtcgtggcat
     6241 taattccgtg gaatatgccc ggactaaaag ccgtcggttc ttatcgctcg gtactggaat
     6301 tgctcaatat tccccatgcg aaattgatca tggactgcgt gatattactt tccgtaacca
     6361 gttgcctgaa ctcggcgctg tataccgctt ccaggatgct ctactcctta agccgtcgcg
     6421 gagatgcgcc tgcggtaatg ggtaaaatca accgcagtaa aaccccgtac gtggcggtgt
     6481 tactctccac cggcgcggca ttcttaacgg tggtggtgaa ctattacgcg cctgcgaagg
     6541 tatttaaatt tctgatcgac agctccggcg ctatcgccct gctggtttat ttagtcatcg
     6601 ccgtttcaca gttgcggatg cgcaaaattc tgcgagcaga aggaagcgaa attcgcttgc
     6661 gcatgtggct ttatccgtgg ctcacctggc tggtcatcgg ctttattacc tttgtgttgg
     6721 tagtgatgct attccgtccg gcgcaacagt tagaagtgat ctccaccggc ttattagcga
     6781 tagggattat ctgtaccgtg ccaattatgg cgcgctggaa aaagctggta ttgtggcaaa
     6841 aaacacccgt tcataatacg cgctgatcat gatcaggagt cacaccatga ccattacgtc
     6901 tctggatggc tatcgctggc tgaagaacga tattattcgc ggtaattttc aaccggatga
     6961 aaaattacga atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc cgttacggga
     7021 agctctttcg caactggtgg cggaacggct ggtcacggtg gtgaatcaaa aagggtatcg
     7081 ggtggcgtct atgtcagagc aggagctgct cgatattttc gacgcccgcg ccaatatgga
     7141 agcgatgtta gtgagtctgg cgattgcccg cggtggcgat gagtgggagg cagacgttct
     7201 cgcaaaagcg catctgctga gtaagcttga ggcctgtgac gccagcgaga aaatgcttga
     7261 tgagtgggat ctgcgtcatc aggcgtttca tacggcaatt gtggcgggct gtggttctca
     7321 ctatttgctg caaatgcgtg aacggttgtt tgatctggcg gcgcgttatc gatttatctg
     7381 gctgcggcga acggtgcttt cggtggaaat gctggaggat aaacacgatc agcaccagac
     7441 cctgactgcg gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa tgcgccagca
     7501 tttactgacg ccaattccca ttatccagca ggcgatggct ggcaattaac tactcttccg
     7561 gaatacgcaa cacttgcccc ggataaattt tatccgggct ttttagcatc ggtttattcg
     7621 cttcgaagat tttattgtac agattagcgt taccgtagac ctgtttggaa atggcactca
     7681 gagtgtcgcc agacttaacg gtataaaact ggctggcagt ggctggtgtc gccgttttca
     7741 cctgatcatc gacactggca ataccggaaa tattccccac cgcaacaagg attttctcct
     7801 tcgcctcctg actcaggccg tcaccagtga ccgtcgcttt gccgtcggca atttgaatat
     7861 tcactttatc ggcatccggt ataccggttt tgttcagatg ctcctgcacc ttcttcgcct
     7921 gatcgtcttt atcgtgctga cctgtaaccg cgtcccagag tttttctccg gcatctttca
     7981 caaaattgaa cagacccata gctacctcat tcgttaacgg aaacactcag aaagtgtagc
     8041 agaggaacgc ggcgcgttaa cgccgcgggg atgagcacag gtttaatcgc gggtttgcac
     8101 ccagaacgcg tgaatcaaac cggggatata cccaagcagt gtcaacagaa tattaataat
     8161 gaacgcccaa ccgaaccctt taccgagcag cacgccgagc ggcggcagaa tgatggtgat
     8221 gacgattctc cagaaaccca tatgtactcc ctataagaaa attactcatt gtttaaaaag
     8281 agattttatc tcttaagcgt agtaatttta gcggagcctg ccagtttcag cttacgcttt
     8341 tactctcatt tacgctgctg aggctggtat atgttgcatt tttagtttag agtttactta
     8401 atttagaaaa tacttaaata aatatgactg aactcgcgca attacaggcc agtgccgaac
     8461 aggcagcggc cttattgaaa gcaatgagcc accctaaacg attgctgatt ctgtgcatgc
     8521 ttagcggttc ccccggcacc agcgcgggag agctgacacg cattaccgga ctgagtgcct
     8581 ctgcgacatc acagcatctc gctcgtatgc gggacgaagg gcttatcgac agccaacggg
     8641 atgcccaacg cattctatat tccattaaaa atgaggcggt aaatgccatt atcgccaccc
     8701 tgaaaaatgt ctattgtccg taaggagtca ctatggcttt gacaaccatt tcgccgcatg
     8761 atgcacaaga attaatcgca cgcggcgcaa agttaatcga tattcgtgat gctgatgaat
     8821 atcttcgtga acatattcct gaagcagatc tggctccatt atccgtgctg gaacagtcag
     8881 gtcttccggc taaattacgt cacgagcaaa ttatattcca ctgccaggca ggtaaacgca
     8941 ccagtaataa tgccgataaa ttagcagcga ttgccgcccc cgcagaaata tttttactcg
     9001 aagatgggat tgatggctgg aaaagagctg gattgccagt agcggtaaat aaatctcaac
     9061 cctttccgtt aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg
     9121 tactgggtta taccgtaaat agcggtttct tcttattaag tggctttgtt ggtgccgggt
     9181 tactgtttgc aggaatcagc ggtttttgtg gaatggcaag gttgttagat aagatgccgt
     9241 ggaaccaacg agcttgagaa gcgacgccgg acgcgcccta gcagcgacat ccggcctcag
     9301 taattagatc aggaaatcgt cgagagattt accttctgcc agcgcctgag caattggctt
     9361 cggtgtacgg ccctgaccgg tccaggtttt agtttcaccg ttaacatcgg tgaatttata
     9421 tttcgccgga cgcggctggc gttttttacc agcgcgtggt gcagcagcag agctattacc
     9481 taataactct tccgggttaa ttccgtcagc cttcatcagc tccagccagg tgctaatttt
     9541 ttcctgacgc tcagccagtt cacgctgctg ctgttcttct tcttcacgtc tttctttagt
     9601 gacaaccctg aatttttcga gcatttcttc aagaacgtca atggagaatt cgcgagccat
     9661 cgcacggaga gtgcgaatgt tatttaaact ttgtaacatt acggacataa taaagaaaac
     9721 cttttaacgc caaaacaaaa aagtatttcg tcgcgtagtg taagcgatca cttattatat
     9781 ttc
//
LOCUS       DAPHJE010000147         9655 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPHJE010000147 DAPHJE010000000
VERSION     DAPHJE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.148"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..1063
                     /gene="iolG"
                     /locus_tag="RH222_003898"
     CDS             77..1063
                     /gene="iolG"
                     /locus_tag="RH222_003898"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDV3798844.1"
                     /translation="MFNIALLGAGRIGQVHAANIASHSATTLWSVVDPNQEFATRLAT
                     QYQARQQSLDEAMTDPNVHAVLIASATDTHADLIEMAARYGKAIFCEKPVHLDLARVR
                     DCLKVVKEYDVPLFIGFNRRFDPQFRRVKTDTQAGRIGKPESLLIISRDPSPPPAEYV
                     RVSGGMFRDMTIHDFDMARFIMGEEPVSVYAQGSNLVDPAIGGAGDIDTAFIVLKYAS
                     GAMATIVNSRRSSYGYDQRLELHGSEGLLCAGNILENQVQHYGKQGCTSALPEHFFLQ
                     RYKSAYAAEWEHFVAVLRGEAVPDCSGDDGERALYLADKALESLRCQREIVL"
     gene            1095..2024
                     /locus_tag="RH222_003899"
     CDS             1095..2024
                     /locus_tag="RH222_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV3798845.1"
                     /translation="MKKLILAALIAMTSGAAIAENEQIVFSTPNLAMPFEVHMQRTAV
                     KAAKEMGVKLQVLDGQGSSPKQVADLENAITRGAQGFIVSPNDVNAVSSAVDEIQDTK
                     LPVVTLDRSVDSQKKVPHFGANNYKGGQAIGDFVKTKFPNGAEIILLTGQPGSSSNIE
                     RTKGIRDSLKAGGDKYKIVADQTGNWMRSEGMRIVESVLPSLPKRPQVILSANDDMAL
                     GAIEALQSQGVQPGEILVTGFDAVPEALARVRDGWLAVTADQRPGFAVTTAMSQLVAN
                     VREKKAITGADYPPTLITKENLQQAERIAEAGN"
     gene            2076..3623
                     /locus_tag="RH222_003900"
     CDS             2076..3623
                     /locus_tag="RH222_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3798846.1"
                     /translation="MSQPLLKVTDLAKSFSGVWALSSAQLTVGTGEIHALLGENGAGK
                     STLLKALAGAQPQTRGEIWFNGETLPVDDSPVERQNKGIITIYQEFNLLPNMTVAENM
                     FLGREPRKRNLIVDEKAVNQEAQVILDYLQLNVAPTTPVARLSVAQQQMVEIARALTL
                     NARLIIMDEPSAALSDSEVESLHRVVRELKNRGVSIIYVTHRLHEVFQLCDRFTVFQD
                     GRFTGTGAVTETNVEQLIRLMVGRDVAFNRRPASETHHEDKPVRLSVKGLSREKPPLD
                     PHGIALHDISFHVHAGEVLGIAGLVGAGRTEVARCLFGADAFTTGSFELDGVPYKPRD
                     PLYALEQGLALVPEDRKKEGAVLGLSIRDNLSLSCLSSLLQWRWFVNTRKEDDLIESY
                     RQALQIKMVNSTQEVRKLSGGNQQKVILARCMALNPRVLIVDEPTRGIDVGTKSEVHQ
                     VLFDMAKKGVAVIVISSDLPEVMAVSDRIITLSEGRLTGEIHGDDANEERLMTMMAIN
                     HNALNAA"
     gene            3635..4663
                     /locus_tag="RH222_003901"
     CDS             3635..4663
                     /locus_tag="RH222_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3798847.1"
                     /translation="MTQMIPKTQVPVKRAGRIDPIAFFERFGVLIFMILLLIFFQSQN
                     SNFFSERNIFNILTEVSIYGIMAVGMTFVILTAGIDLSVGSILAVCAMTAAYVIKGDN
                     FTTVDPNAWGGMSWLIGLGICLTMGTAIGFLHGLGVTRLRLPPFIVTLGGMTIWRGLT
                     LVINDGAPIAGFDQGYRWWGRGELLGISIPIWIFAIVAIGGYLALHKTRWGRFVYAIG
                     GNPEAARLAGVNVKRVLVSVYVLIGCLAGLAGFILSARLGSAEAVAGISFELRVIASV
                     VIGGTSLMGGYGRIGGTIIGSIIMGILINGLVLMNVSAYYQQIITGLIIVLAVAFDTY
                     AKSRRGAL"
     gene            4672..5805
                     /locus_tag="RH222_003902"
     CDS             4672..5805
                     /locus_tag="RH222_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3798848.1"
                     /translation="MKEVRIGLIGTGYIGKAHAIAYAQAPTVFNLRGKLVREMVAEVN
                     PALAAERAQAFGFNRSTGDWRVLVADPAIDVVDICSPNHLHKEMALEAIRHGKHVYSE
                     KPLALNAHDAREMVDAAKRAGVKTLVGFNYMKNPTAQLAKEIIARGEIGEVIHFYGTH
                     NEDYMADPLSPIHWHCFKETAGLGALGDLAAHIVNMAQYLVGEIEQVCGDLKIVVPAR
                     PAKAGSSEMVTVENEDQAHAMVRFAGGAQGVIETSRVACGRKMGLSYVITGTKGAISF
                     TQERMAEIKLYLHDDPVNRQGFRTLLVGPAHPDYGAFCMGAGHGIGFNDQKTVEVRDL
                     VDGIATDAPMWPDFEEGWKVSRVLDAIALSHQQGLWLNVNDIV"
     gene            5818..7722
                     /gene="iolC"
                     /locus_tag="RH222_003903"
     CDS             5818..7722
                     /gene="iolC"
                     /locus_tag="RH222_003903"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDV3798849.1"
                     /translation="MEKQFDVICMGRVAVDLYSQQIGARLEDVSSFAKYLGGSSGNVA
                     YGTARQGLRSSMLARVGDEHMGRFLREELNQVGCDTSHLITDKERLTALVLLGIKDRD
                     TFPLIFYRDNCADMAITASDVDESYIASARCIAITGTHLSHPQTREAVLTALNYARRH
                     GVRTVLDIDYRPVLWGLTSLGDGETRFIVAEQVTRELQEVLHLFDVVVGTEEEFHIAG
                     GSTDTLQALAQVRHVSKATLVCKRGALGCSVYTDAIPPRLDDGLTVTGVRVEVLNVLG
                     AGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPSKIELDDYLSRAAL
                     VPRPDIDPRLNHLHRVTTRRREWPELCVMAFDHRSQLEDMALQCGASLKRIPALKQLI
                     LQASREAASSAGLEGKAGLLCDGTFGQDALNAITGEGWWIGRPIELPGSRPLEMEHGN
                     IGTQLISWPQEHVVKCLVFFHPEDAHGLRLEQEQKIAEVYHACCQSGHELLLEVILPA
                     TMPRSDELYLRAISRFYNLGIYPDWWKLPPLSAEGWTALSEIIARRDPHCRGVVILGL
                     DAPAEQLRADFKAAAGQALVKGFAVGRTLFGDASRAWLKHDIDDAQLVARIRDNYLQL
                     IAWWRERGHA"
     gene            7734..8624
                     /gene="iolE"
                     /locus_tag="RH222_003904"
     CDS             7734..8624
                     /gene="iolE"
                     /locus_tag="RH222_003904"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010433880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDV3798850.1"
                     /translation="MSVQLGINPLTWTNDDLPSLGAETSLETCLSEGKEAGFAGFELG
                     NKFPREARLLGPILQRHDLQLVSGWYSGRLLERSVEDEIAAVQSHLTLLRELGAKVLV
                     FAEVSGCIHGEQQTPVHLRPRFPQECWKEYGEKLTVFARYTQQQGVQIAYHHHMGTVI
                     ESAQDVDNLMLHTGEEVGLLLDTGHLTFAGADPLAVAQRWASRINHVHCKDVRADVLA
                     DVKNRKTSFLDAVLSGVFTVPGDGCVDYPPIMQLLKTQDYHGWLVVEAEQDPAIAHPL
                     TYARLGYNNLSRLARDAGLI"
     gene            8634..9455
                     /gene="iolB"
                     /locus_tag="RH222_003905"
     CDS             8634..9455
                     /gene="iolB"
                     /locus_tag="RH222_003905"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDV3798851.1"
                     /translation="MSRLLSRWQQPNAEGRTQSVTPESAGWGYVGFEAYELEEGQQLT
                     LPAVSEERCLVLVAGRAFISTPSAQFIDIGERMSPFERIKPWAVYVTPQEAVQVKALT
                     KLELAVCTAPGKGTYPTRLIAPQDIDGEARGNGHNQRYVHNILPEDKPADSLLVVEVW
                     TNEGCTSSYPSHKHDTNNPPQESYLEETYYHRLNPEQGFCMQRVYTDDRTLDECMAVY
                     NRDVVMVPKGYHPVATMAGYDSYYLNVMAGPVRKWIFTWEKEHSWINTEYSAISL"
BASE COUNT         2041 a         2613 c         2960 g         2041 t
ORIGIN      
        1 ctatttataa aaatgaaata aatgttttat ttgtagtgtc gttagttcac cgcattcatc
       61 aacaccggga gccgttatgt ttaacattgc tttactgggc gctggccgaa ttggccaggt
      121 acatgcagct aacatcgcca gccatagtgc gaccacgctc tggtctgtgg tagacccgaa
      181 tcaggagttc gccacgcgtc tcgcgacaca gtatcaggcg cgccagcaga gcctggatga
      241 ggcgatgacc gacccgaacg ttcatgccgt attgatcgcc tccgcgaccg acacccacgc
      301 ggatctgatt gaaatggccg cccgttacgg caaagccatt ttctgtgaaa aaccggtaca
      361 ccttgatctt gcccgcgtac gcgactgcct gaaagtggtc aaagagtatg acgtaccgct
      421 gtttatcggt tttaaccgcc gctttgaccc acagttccgt cgcgtgaaaa ccgacactca
      481 ggccgggcgt atcggcaaac cggaatcact gctgatcatc tcccgcgatc cgtctccacc
      541 gcctgcggag tacgtgcgtg tttccggcgg catgttccgt gatatgacca tccacgactt
      601 tgacatggca cgcttcatca tgggtgaaga gccggtgtcg gtgtatgcac agggcagcaa
      661 tctggtggat ccggcgattg gcggagcggg agatatcgac accgcattta tcgttctgaa
      721 atatgcctcc ggcgcgatgg cgaccatcgt caacagtcgc cgctcttcat acggctacga
      781 ccagcgcctg gagctacacg gttccgaagg gctgctgtgc gcgggcaata tccttgaaaa
      841 ccaggtgcag cactacggca aacagggttg caccagcgcg ctgccggaac acttcttcct
      901 gcaacgctac aaatccgctt acgcagcgga atgggagcac tttgttgcgg ttctgcgcgg
      961 cgaagcggtg cctgattgca gcggcgatga tggtgaacgt gcgctgtacc tagcggataa
     1021 agcgctggaa tcactgcgtt gccagcgtga gatcgtcctc taaacacaac aaccctacac
     1081 agagagacat aataatgaaa aaactgatct tagccgccct aatcgccatg acatccgggg
     1141 ccgccatagc cgaaaacgag caaattgttt tcagtacgcc aaacctggcc atgccgtttg
     1201 aagttcacat gcagcgcacg gcggtgaaag ccgcaaaaga gatgggggtg aaacttcagg
     1261 tactggacgg tcagggcagc tcaccgaagc aggtggctga cctggaaaac gccatcaccc
     1321 gtggtgcgca gggctttatc gtctccccga acgatgtgaa cgcggtctcc agtgcggtag
     1381 atgaaattca ggatacgaag ctgccggtcg ttacgcttga ccgctctgtc gacagccaga
     1441 aaaaagtgcc gcactttggt gccaacaact acaagggcgg ccaggcgatt ggcgacttcg
     1501 tcaaaaccaa attccccaac ggcgcggaga tcattctgtt gaccggtcaa ccgggctcct
     1561 cctccaatat tgaacgcacc aaagggatcc gcgacagcct gaaggcgggc ggggataagt
     1621 acaaaatcgt cgccgatcag accggcaact ggatgcgttc tgaagggatg cgcattgtcg
     1681 aaagcgtttt accgtcgctg ccaaaacgtc cgcaggtgat cctctccgcc aacgacgata
     1741 tggcgctggg ggcgattgaa gcccttcaga gtcagggtgt gcaaccgggt gaaatcctgg
     1801 tcaccggctt tgatgcggta ccggaagcgc tggcccgcgt gcgcgacggc tggctggcgg
     1861 ttacggcgga tcagcgtccc ggctttgcgg tgaccacggc gatgagccaa ctggtggcta
     1921 acgtgcgtga gaaaaaagcc atcaccgggg cggactatcc gccgacgctg atcaccaaag
     1981 agaacctgca acaggctgag cgtattgctg aggccggtaa ctgatttgtc cggagccgcc
     2041 gtacgggcgg ctccccataa ataaggagca ggctgatgtc gcaaccctta ctgaaagtaa
     2101 ccgacctggc aaaaagcttt tccggcgtat gggcgctgag cagcgcccag ctcaccgtcg
     2161 gcacgggtga aattcacgct ttgctgggcg aaaacggcgc tgggaaatcc acgctgttga
     2221 aggcactcgc gggcgcacag ccacaaacgc gcggggagat ctggttcaat ggcgaaacgc
     2281 tgccggtgga cgactccccg gtagaacggc agaacaaggg cattatcacc atctatcagg
     2341 aattcaacct gctgcccaat atgaccgttg cggaaaacat gtttctcgga cgcgaaccgc
     2401 gtaaacgtaa cctgatcgtc gacgaaaaag cggtcaatca ggaagcgcag gtgattctcg
     2461 attatctgca actgaatgtc gcacccacca cgcccgtcgc ccgtctgagc gtggcccagc
     2521 agcagatggt ggagattgcc cgggcgctga ccctcaatgc caggctgatc atcatggatg
     2581 aaccctccgc cgccctgagt gacagcgagg tggaaagcct gcaccgcgtg gtgcgggagc
     2641 ttaaaaaccg cggcgtgagc attatttacg ttactcaccg tctgcatgaa gttttccagc
     2701 tttgcgatcg tttcaccgtt ttccaggatg gccgtttcac cggcaccggc gcagtgacgg
     2761 agacgaacgt agagcagttg atccgcctga tggtagggcg tgatgtggcc tttaaccgcc
     2821 gccctgccag cgaaacccac catgaagaca aacctgtccg tttgtcggtg aaggggttaa
     2881 gccgggaaaa accgccgctg gatccacatg gcatcgccct gcatgacatc agttttcacg
     2941 tccatgccgg ggaagttctg ggtatcgctg ggctggtggg ggctggtcgt actgaggtag
     3001 cgcgctgcct gtttggcgcc gacgccttta ccactggcag ctttgagctg gacggcgtgc
     3061 cgtacaagcc gcgtgaccca ctttatgcgc tggagcaagg gctggcgctg gtgccggaag
     3121 atcgcaaaaa agagggggcg gtgctgggtc tgtcgattcg cgacaacctg tcgctctcct
     3181 gcctctcctc gctgttgcag tggcgctggt ttgtgaatac ccgcaaagag gacgacctga
     3241 ttgagtccta ccgccaggcg ctgcagatca aaatggtcaa cagcacgcag gaggtgcgta
     3301 agctctctgg cggcaaccag cagaaagtta tcctcgcgcg ctgcatggcg ctcaatccac
     3361 gggtgttgat tgtcgatgag ccgacacgtg gcatcgacgt tggaaccaaa tcggaagtgc
     3421 accaggtgct gtttgatatg gcgaaaaagg gcgtggcggt gatcgtgatc tcctccgatc
     3481 tgcctgaagt gatggcggtg tctgaccgga tcatcaccct tagtgaaggc cggttaactg
     3541 gcgagatcca cggtgacgac gctaacgaag aacggctgat gacgatgatg gctatcaacc
     3601 acaacgccct gaacgcggca taacggagtt tctcatgacg cagatgattc ctaaaacgca
     3661 ggtcccggta aaacgcgccg gacgcatcga tcccattgcc ttcttcgagc gcttcggggt
     3721 actgattttc atgatcctgc tgctgatctt ctttcagtcg cagaacagta attttttctc
     3781 tgaacgcaat attttcaaca tcctgaccga agtctccatc tacgggatca tggccgtggg
     3841 gatgaccttc gtcattctga ccgctgggat cgacctctcc gtgggctcta ttctggcagt
     3901 gtgcgccatg acagcggcgt acgtgatcaa aggcgacaat ttcaccacag tcgatccgaa
     3961 cgcttggggc ggtatgagct ggctgattgg gctggggatt tgtctgacca tgggtacagc
     4021 gattggcttc ctgcacggtc tgggcgtcac ccgcctgcgc ctaccaccgt tcatcgtcac
     4081 ccttggcggg atgaccatct ggcgtggtct gacgctcgtg attaacgacg gtgcgccgat
     4141 tgccggcttc gatcagggtt atcgctggtg gggacgaggg gaattgctcg gcatctccat
     4201 tccgatctgg atcttcgcca tcgttgccat tggtggttat ctggcactgc ataaaacccg
     4261 ttgggggcgc ttcgtctatg ccatcggtgg caacccggag gcggcacgtc tggctggggt
     4321 aaacgtgaag cgtgtgctgg ttagcgtcta tgtgctgatt ggctgtctgg cggggctggc
     4381 gggcttcatc ctgagcgctc gtctggggag cgctgaggcg gttgccggga tctcctttga
     4441 gctgcgtgtt attgcctcgg tggttattgg cggcacgtcg ttgatggggg gatatgggcg
     4501 catcggcggc actatcatcg gctccatcat catgggcatt ctgattaatg gcctggtact
     4561 gatgaacgtc tcggcctact accagcaaat tatcaccgga ttaattatcg ttctggcggt
     4621 ggcattcgac acctatgcca agagccgtcg cggcgcactg tgaggatcac gatgaaagag
     4681 gttcgtattg gattgattgg caccgggtat attggcaagg cgcatgccat tgcctatgcc
     4741 caggcaccga cggtgttcaa tctgcgcggc aaactggtgc gcgagatggt ggctgaggtg
     4801 aacccggcgc tggcggcaga acgcgcgcag gcgtttggct tcaatcgctc aaccggagac
     4861 tggcgggtgc tcgtggccga cccggccatt gatgtggtgg atatttgctc ccctaaccat
     4921 cttcacaaag agatggcgct ggaggcgatc cgccacggca agcacgtcta ttcggaaaag
     4981 ccgctggcac tgaatgccca cgacgcgcgt gagatggtcg acgctgcgaa gcgggcaggg
     5041 gtgaaaacgc tggtgggttt caattacatg aaaaacccga cggcacagtt ggcgaaagag
     5101 attattgcgc gcggcgaaat tggtgaggtg atccacttct acggaaccca caacgaagac
     5161 tatatggccg acccgctttc ccctattcac tggcactgtt ttaaagagac cgccggactg
     5221 ggggctctgg gtgacctggc ggcacatatc gtcaatatgg cccagtatct ggtgggagag
     5281 attgagcagg tctgtggcga tctgaaaatt gtggtgccgg cacgtccggc aaaggccgga
     5341 tcgtcggaaa tggtcaccgt ggaaaatgaa gatcaggccc acgcgatggt gcgctttgcg
     5401 ggcggggcgc agggcgtcat tgaaacttcc cgcgtcgcct gcggccgcaa gatggggctg
     5461 tcatacgtca ttactggtac caaaggagcg atcagtttta cccaagagcg gatggcagag
     5521 ataaagctct atctacatga tgatccggtg aaccgtcagg ggttccggac actgttggtc
     5581 ggtccggcac acccggatta cggcgcgttt tgcatgggcg caggtcatgg tatcggcttt
     5641 aacgatcaaa aaacggtgga agtgcgtgat ctggtagacg gcattgccac cgacgcaccg
     5701 atgtggccgg acttcgaaga gggctggaag gtctcgcgcg tgctggatgc catcgcactc
     5761 tcacaccagc agggcctctg gctgaacgtg aatgacattg tctgaagagg gttcaacgtg
     5821 gaaaagcagt ttgatgtgat atgcatgggc cgcgtggcgg tagacctcta cagccagcaa
     5881 attggtgccc gcctggagga tgtgtcgagc ttcgccaaat atctcggcgg ctcgtccggc
     5941 aacgtggcgt acggtaccgc gcgtcagggc ttacgctcgt caatgctggc gcgcgttggc
     6001 gatgaacata tggggcgctt cctgcgggaa gagttaaacc aggtgggctg cgataccagc
     6061 catctgatca ccgataaaga acgccttacg gcgctggtgc tactcggcat taaagaccgg
     6121 gacacctttc ctctgatttt ttatcgcgat aactgcgcgg atatggcgat tactgccagc
     6181 gatgtggacg aaagctacat tgcctccgca cggtgtattg ccatcaccgg gactcacctt
     6241 tctcacccgc agacccgcga ggcggtgctg acggcgctga attacgcccg ccgtcacggc
     6301 gtgcgcacgg tgctggacat cgactaccgt ccggtgctgt ggggactaac ctctctagga
     6361 gacggcgaaa cgcgttttat cgttgccgaa caggttaccc gtgaacttca ggaggtgcta
     6421 caccttttcg acgtcgttgt cggcaccgaa gaggagtttc acattgcggg gggcagcacc
     6481 gatacgttgc aggcgctggc gcaggtgcgc cacgtcagca aggcgacact ggtttgcaag
     6541 cgcggcgcgc tgggctgttc ggtgtatacc gatgccattc cacctcggct ggatgacggc
     6601 ctgacggtga ccggcgtgcg tgtggaggtg cttaacgtcc ttggcgcggg agatgcgttt
     6661 atgtctggcc tgctgcgcgg ttacctgaac gacgagggct gggagcaggc gtgccgctac
     6721 gccaacgcct gtggggcgct ggtggtctcg cgccacggct gtgcaccggc gatgccgagc
     6781 aaaattgagc tggatgatta cctctcccgc gccgcgctcg ttccgcgccc ggacattgac
     6841 ccgcgtctta accatctgca ccgggtgacc acccgccgcc gcgaatggcc ggagctgtgc
     6901 gtgatggcgt tcgatcaccg cagtcaactg gaagatatgg cgctgcagtg cggcgcgtcg
     6961 cttaagcgta ttccggcgct gaaacagctg atcctgcagg ccagccgaga ggcggcaagc
     7021 tctgccgggc tggaggggaa agccggcctg ctgtgcgatg gcacctttgg tcaggatgcg
     7081 ctgaacgcca ttaccggcga agggtggtgg attggtcgtc ccattgaact gccaggctcg
     7141 cgtccgctgg agatggagca cggtaacatt ggtacccagc ttatcagttg gccgcaggag
     7201 catgtggtga agtgcctggt ctttttccat ccggaagatg cccacggctt gcgtctggag
     7261 caggagcaga aaatcgccga ggtgtaccac gcctgctgtc agtccggaca tgaactgctg
     7321 ctggaggtga tattacccgc cactatgccg cgcagtgacg aactttatct acgtgccatt
     7381 tcccgcttct acaaccttgg catttacccc gactggtgga aactgccgcc gctctccgcc
     7441 gaaggctgga cggcactgag cgagattatc gcgcgccggg acccgcactg ccgcggcgtg
     7501 gtgatcctcg gtctggatgc tccggcggaa cagctgcgtg ccgacttcaa agctgccgcg
     7561 gggcaggcgc tggtgaaagg cttcgccgtg gggcgcacgc tgtttggcga tgcctcccgc
     7621 gcatggctga aacacgatat tgacgatgcg cagctggtgg cgcgcatccg ggacaactac
     7681 ctgcagctta tcgcctggtg gcgcgagcgc ggacacgcat aatggagacg acaatgagtg
     7741 tacaactggg catcaacccg ctgacgtgga cgaacgatga tctgccttca ctgggcgcgg
     7801 agacatctct ggagacctgc ctgagtgaag ggaaagaggc cggttttgcc ggcttcgaac
     7861 tgggcaataa attcccgaga gaagcgcgcc tgctcggccc cattttgcag cgccacgacc
     7921 tgcagttggt ctccggctgg tattccggcc gcctgttgga acgcagtgta gaggacgaga
     7981 ttgctgctgt gcagtcgcat ctgacgctgc tgcgagagct gggtgcaaaa gtactggttt
     8041 ttgcggaagt gagtggctgc attcacggtg agcagcagac gccggttcac ctgcgcccac
     8101 gtttcccgca ggaatgctgg aaagagtatg gtgagaagct caccgtgttc gcccgctata
     8161 cgcagcaaca gggggtacag attgcttacc accatcatat gggcacggtg attgagtccg
     8221 ctcaagacgt ggacaacctg atgctccaca cgggtgagga ggttggttta ctgctggata
     8281 ctggccacct gacctttgcc ggggccgatc cgctggcggt ggcacagcga tgggcatcgc
     8341 gcatcaatca tgttcactgc aaagatgtgc gcgccgatgt gctggcggac gtcaaaaacc
     8401 gtaaaaccag cttcctcgac gcggtgctga gcggcgtgtt taccgtgccg ggtgacggct
     8461 gcgtggatta tccgccgatt atgcaactgc tgaaaacgca ggattatcac ggttggctgg
     8521 tggtggaggc tgaacaggac ccggcgatcg cccatccgct gacctacgcc cgtctgggat
     8581 ataacaatct gagccgcctg gcgcgcgacg ccgggcttat ctgaggaggt gatatgtcac
     8641 gtctgttatc acgctggcaa cagccgaacg ctgaggggcg gactcagtct gtcacgccgg
     8701 aaagcgccgg atggggatat gttggctttg aggcctatga acttgaggaa gggcagcaac
     8761 tgacgctgcc cgctgtcagt gaagagcgct gtctggtgct ggtggccggg cgcgctttca
     8821 tcagcactcc ttctgcacag tttattgata tcggggagcg gatgagcccg ttcgagcgca
     8881 tcaaaccttg ggcggtttac gtcaccccgc aggaggcagt gcaggtgaag gcgctgacga
     8941 aactggagct ggcagtctgt accgctccgg gaaaagggac atacccgacg cggttgattg
     9001 cacctcagga tatcgatggc gaagcgcgcg gtaacggcca caaccagcgt tatgtgcata
     9061 atattttgcc ggaagacaag cccgctgaca gcctgctggt cgtcgaggtc tggaccaacg
     9121 aaggctgtac cagttcgtac ccgagccata aacacgatac gaataacccg cctcaggaga
     9181 gctatctgga ggagacctat taccatcgcc tcaacccgga acaggggttc tgcatgcagc
     9241 gggtgtatac cgatgaccgc acacttgatg aatgtatggc ggtctataac cgtgacgtgg
     9301 tcatggtgcc gaaagggtac cacccggtgg cgacgatggc cgggtatgac agttattacc
     9361 ttaacgtgat ggccggacca gtacgtaaat ggatattcac ctgggaaaag gaacactcat
     9421 ggataaacac agagtattct gccatttctt tataaactga gtacctaagc taaaaaaggg
     9481 gaatgactcc ccttttgtat aatatgaact cgacaatgtt atttcaaagt ctgagtgatg
     9541 atttttcgct caggcagtat gttcctcaat aaggatgaga atgtattctg ttcattggca
     9601 tatgcattta tattctaatg agctgaattt tataaaggtt gtggctattc tggag
//
LOCUS       DAPHJE010000148         9578 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPHJE010000148 DAPHJE010000000
VERSION     DAPHJE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.149"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            83..1057
                     /gene="cysB"
                     /locus_tag="RH222_003906"
     CDS             83..1057
                     /gene="cysB"
                     /locus_tag="RH222_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV3798852.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1352..1516
                     /locus_tag="RH222_003907"
     CDS             1352..1516
                     /locus_tag="RH222_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV3798853.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1519..1686
                     /locus_tag="RH222_003908"
     CDS             1519..1686
                     /locus_tag="RH222_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798854.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1800..1895
                     /locus_tag="RH222_003909"
     CDS             1800..1895
                     /locus_tag="RH222_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798855.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2059..4734
                     /gene="acnA"
                     /locus_tag="RH222_003910"
     CDS             2059..4734
                     /gene="acnA"
                     /locus_tag="RH222_003910"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV3798856.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4798..5388)
                     /gene="ribA"
                     /locus_tag="RH222_003911"
     CDS             complement(4798..5388)
                     /gene="ribA"
                     /locus_tag="RH222_003911"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV3798857.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5558..6322
                     /gene="pgpB"
                     /locus_tag="RH222_003912"
     CDS             5558..6322
                     /gene="pgpB"
                     /locus_tag="RH222_003912"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV3798858.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6471..6779
                     /gene="lapA"
                     /locus_tag="RH222_003913"
     CDS             6471..6779
                     /gene="lapA"
                     /locus_tag="RH222_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV3798859.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6786..7955
                     /gene="lapB"
                     /locus_tag="RH222_003914"
     CDS             6786..7955
                     /gene="lapB"
                     /locus_tag="RH222_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV3798860.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8148..8885
                     /gene="pyrF"
                     /locus_tag="RH222_003915"
     CDS             8148..8885
                     /gene="pyrF"
                     /locus_tag="RH222_003915"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV3798861.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8885..9211
                     /gene="yciH"
                     /locus_tag="RH222_003916"
     CDS             8885..9211
                     /gene="yciH"
                     /locus_tag="RH222_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV3798862.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9337..9555)
                     /gene="osmB"
                     /locus_tag="RH222_003917"
     CDS             complement(9337..9555)
                     /gene="osmB"
                     /locus_tag="RH222_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV3798863.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2375 a         2251 c         2492 g         2460 t
ORIGIN      
        1 ttttattatt aaatcgtatt agtcacccgc caggtgtgac gaaaaaacga tgttctgatg
       61 gcgtctaagt ggatggttta acatgaaatt acaacaactt cgctatattg ttgaggtggt
      121 caatcataac ctgaatgtct catcaacagc ggaaggactt tacacatcac aacccgggat
      181 cagtaaacaa gtcagaatgc tggaagacga gctaggcatt caaatttttt cccgaagcgg
      241 caagcacctg acgcaggtaa cgccagcagg acaagaaata attcgtatcg ctcgcgaagt
      301 cctgtcgaaa gtcgatgcca taaaatcggt tgccggtgag cacacctggc cggataaagg
      361 ctcgctgtat atcgccacca cgcataccca ggcacgctac gcgttaccaa acgttatcaa
      421 aggctttatt gagcgttatc cccgcgtttc tttgcatatg caccagggct cgccgacaca
      481 aattgctgat gccgtctcta aaggcaatgc tgatttcgct atcgccacag aagcgctgca
      541 tctgtatgaa gatttagtga tgttaccgtg ctaccactgg aatcgggcta ttgtagtcac
      601 tccggatcac ccgctggcag gcaaaaaagc cattaccatt gaagaactgg cgcaatatcc
      661 gttggtgaca tataccttcg gctttaccgg acgttcagaa ctggatactg cctttaaccg
      721 cgcagggtta acgccgcgta tcgtcttcac ggcaacggat gctgacgtca ttaaaactta
      781 cgtccggtta gggctggggg taggggtcat tgccagcatg gcggtggatc cggtcgccga
      841 tcccgacctt gtgcgtgttg atgctcacga tatcttcagc cacagtacaa ccaaaattgg
      901 ttttcgccgt agtactttct tgcgcagtta tatgtatgat ttcattcagc gttttgcacc
      961 gcatttaacg cgtgatgtcg ttgatgcggc tgtcgcattg cgctctaatg aagaaattga
     1021 ggtcatgttt aaagatataa aactgccgga aaaataattt cttgcgttat tttcggcacc
     1081 ttttatgtag cgaaggtgcc ggaatatatt ctcttttgtt acttatttct ataccgtgca
     1141 caatattttt atccctcata tttatagggt aaattacctg taaagtagtg tctaattacc
     1201 cgcctcgcaa actccagttt tcaaatatct atttcaggtc aaaagattga attattcact
     1261 ggagacgatt cgtaaattcg ctggattttt gactaaagtt tctttaggat ttgtctcatc
     1321 gtatgattaa ttacaccaat cgttcaacgt cttgctgata agtgatggcg attatatgag
     1381 gttagcaatg ccttctggaa atcaggaacc ccgccgcgat cctgaattaa aacgtaaagc
     1441 ctggctggcg gtttttcttg gttctgcact tttctgggtg gttgtcgcac tgctgatttg
     1501 gaaagtgtgg ggataactat ggtcggtcag gagcaactgg agtcgtcacc attatgccag
     1561 catagtgaca atgaaacaga aacgaaacgg gaatgttccg tcgttattcc agacgactgg
     1621 caactaacat cgcagcagca agcctttata gaactgtttg ctgaagatga tcagccgaaa
     1681 caataattat catcattctt attacccatt tttaatgaat taaagggctt ttaatacacc
     1741 gcagcaataa cagcttgagt tatctcaaca caaaataata accgttaagg gtgtagccta
     1801 tgatcaacac aaatatgaaa tattggtcct ggatgggcgc gttttctctg tcgatgctct
     1861 tctgggccga actcctctgg atcattactc actgatcctt gaccccgctg cggcggggtt
     1921 gtcatttgct ttgccacaag gtttctcctc ttttatcaat ttgggttgtt atcaaatcgt
     1981 tacgcgatgt ttgtgttatc tttaatattc accctgaaga gaatcagggc ttcgcaaccc
     2041 tgtcattaag gaggagctat gtcgtcaacc ctacgagaag ccagtaagga cacgttgcag
     2101 gccaaagata aaacttacca ctactacagc ctgccgcttg ctgctaaatc actgggcgat
     2161 atcacccgtc tgcccaagtc actcaaagtt ttgctcgaaa acctgctgcg ctggcaggat
     2221 ggtaactcgg ttaccgaaga ggatatccac gcgctggcag gatggctgaa aaatgcccat
     2281 gctgaccgtg aaattgccta ccgcccggca agggtgctga tgcaggactt taccggcgta
     2341 cctgccgttg ttgatctggc ggcaatgcgc gaagcggtta aacgcctcgg cggcgatact
     2401 gcaaaggtta acccgctctc accggtcgac ctggtcattg accactcggt gaccgtcgat
     2461 cgttttggtg atgatgaggc atttgaagaa aacgtacgcc tggaaatgga gcgcaaccac
     2521 gaacgttatg tgttcctgaa atggggaaag caagcgttca gtcggtttag cgtcgtgccg
     2581 ccaggcacag gcatttgcca tcaggttaac ctcgaatatc tcggcaaagc agtgtggagt
     2641 gaattgcagg acggtgaatg gattgcttat ccggatacac tcgttggtac tgactcgcac
     2701 accactatga tcaacggcct tggcgtgctg gggtggggcg tgggtgggat cgaagcagaa
     2761 gccgcaatgt taggccagcc ggtttccatg cttatcccgg acgtagtggg ctttaaactt
     2821 accggtaaat tacgtgaagg tattaccgcc acagacctgg ttctcactgt tacccaaatg
     2881 ctgcgcaaac atggcgtggt ggggaaattc gtcgaatttt atggtgatgg tctggattca
     2941 ctaccgctgg cggatcgcgc caccattgcc aatatgtcgc cagaatatgg tgccacctgt
     3001 ggcttcttcc caatcgatgc tgtaaccctc gattacatgc gtttaagcgg gcgcagcgaa
     3061 gatcaggttg agttggtcga aaaatatgcc aaagcgcagg gcatgtggcg taacccgggc
     3121 gatgaaccga tttttaccag tacgttagaa ctggatatga atgacgttga agcgagcctg
     3181 gcagggccaa aacgcccaca ggatcgcgtt gcactgcccg atgtaccaaa agcatttgcc
     3241 gccagtaacg aactggaagt gaatgccacg cataaagatc gccagccggt cgattatgtt
     3301 atgaacggac atcagtatca gttacctgat ggcgctgtgg tcattgctgc gataacctcg
     3361 tgcaccaaca cctctaaccc aagtgtgctg atggccgcag gcttgctggc gaaaaaagcc
     3421 gtaactctgg gcctcaagcg gcaaccgtgg gtcaaagcgt cgctggcacc gggttcgaaa
     3481 gtcgtttctg attatctggc aaaagcgaaa ctgacaccgt atctcgacga actggggttt
     3541 aaccttgtgg gatacggttg taccacctgt attggtaact ctgggccgct gcccgatcct
     3601 atcgaaacgg caatcaaaaa aggcgattta accgtcggtg cggtgctgtc cggcaaccgt
     3661 aactttgaag gccgtatcca tccgctggtt aaaactaact ggctggcctc gccgccgctg
     3721 gtggttgcct atgcgctggc gggaaatatg aatatcaacc tggctgctga gcctattggt
     3781 catgatcgta aaggcgaccc ggtttatctg aaagatatct ggccatcggc acaagaaatt
     3841 gcccgtgcgg tagaacaagt ctccacagaa atgttccgca aagagtacgc agaagttttt
     3901 gaaggcacag cagagtggaa ggaaattaac gtcacacgat ccgataccta cggttggcag
     3961 gaggactcaa cctatattcg cttatcgcct ttctttgatg aaatgcaggc aacaccagca
     4021 ccagtggaag atattcacgg tgcgcggatc ctcgcaatgc tgggggattc agtcaccact
     4081 gaccatatct ctccggcggg cagtattaag cccgacagcc cagcgggtcg atatctacaa
     4141 ggtcggggtg ttgagcgaaa agactttaac tcctacggtt cgcggcgtgg taaccatgaa
     4201 gtgatgatgc gcggcacctt cgccaatatt cgcatccgta atgaaatggt gcctggcgtt
     4261 gaagggggga tgacgcggca tttacctgac agcgacgtag tctctattta tgatgctgcg
     4321 atgcgctata agcaggaaca aacgccgctg gcggtgattg ccgggaaaga gtatggatca
     4381 ggctcaagcc gtgactgggc ggcaaaaggt cctcgtctgc ttggaattcg tgtggtgatt
     4441 gccgaatcgt ttgaacgaat tcaccgttcg aatttaattg gcatgggcat cctgccgctg
     4501 gaatttccgc aaggcgtaac gcgtaaaacg ttaggactaa ccggggaaga gaagattgat
     4561 attggcgatc tgcaaaatct acaacccggt gcgacggttc cggtgacgct tacgcgcgcg
     4621 gatggtagcc aggaggtcgt accctgccgt tgtcgtatcg acaccgcgac ggagttgacc
     4681 tactaccaga acgacggcat tttgcattat gtcattcgta atatgttgaa gtaacaagta
     4741 tttgcttgcc ggttattttg cttccggcaa gcaaactaat tacacaatgc aagagggtta
     4801 tttgttcagc aaatggccca ttttctcggc tttggtatcg agataatgtt cgttattggg
     4861 gttacgacct acaatcaatg gtacgcgttc aacaatatta atccctgctt cggtcagaat
     4921 ttcgactttt ttcgggttat tggttaacaa gcggacttca ttgacgccaa ggagtttgaa
     4981 catatcagcg caaagagtga agtcgcgctc atcagcggcg aagcctaact ggtggttagc
     5041 ctctacggta tcgtaacctt gatcctgcag tgcgtaagcg cggattttat tcagcagacc
     5101 aatgttacga ccttcctgac ggtgatacag caaaatacca cggccttcct cggcaatttg
     5161 cgtcaatgcc gcttcgagct ggaagccaca atcgcagcgc aagctgaaca gggcgtcacc
     5221 ggtcagacat tcggaatgga cgcgcgcaag taccggggta tgcccggaaa tatcgccata
     5281 gactagcgcg acatgatcgt gtccggttgc cagttcttca aatcccacca tcaggaaatc
     5341 gccccatggg gttggcagtt tggcttctgc cacacgttta agctgcatga aattctccag
     5401 ataatgctgg ttctgtattg gcttattttg ccataacgag aaggggttca cctaatcacg
     5461 acgcgtcgat cgttcacgga atggcacaaa tctgtcaatt tttcctggaa ctggcgtttt
     5521 cagttatgat tgtgggactt atcaaaaagg agaggccatg cgttcgattg ccagacgtac
     5581 cgcagtggga gctgcactat tgcttgtcat gccagtagcc gtatggattt ctggctggcg
     5641 ttggcaacct ggagaacaaa gttggctact aaaagcggct ttttgggtta ctgaaactgt
     5701 cacccagccc tggggcgtca ttacacattt gattttattc ggctggtttc tctggtgtct
     5761 gcgttttcgc attaaggctg cctttgtatt atttgccatt ctggcggccg caatccttgt
     5821 gggacaaggc gttaaatcct ggatcaaaga caaagtccag gaaccacgac cttttgttat
     5881 ctggctggaa aaaacacatc atattccggt tgatgagttc tacactttaa agcgagcaga
     5941 acgcggaaat ttagtgaaag aacagttggc tgaagagaaa aatatcccac aatatttgcg
     6001 ttcacactgg cagaaagaga cggggtttgc ctttccttcc ggtcacacga tgtttgctgc
     6061 cagttgggca ctgctggccg ttggtttgct gtggccgcgt cggcgaacgt taaccattgc
     6121 tatcttgctg gtctgggcaa cgggagtcat gggaagccgc ctgctgctcg ggatgcattg
     6181 gccacgcgat ctggtagtag ctacgttgat ttcgtgggcg ctggtggcgg tggcaacgtg
     6241 gcttgcgcaa cgaatttgtg ggccattaac accacctgcg gaagaaaatc gcgaaatagc
     6301 gcaacgagaa caagaaagtt aacgctggtt gattttccga atttagccct taaatcatca
     6361 acaatgcgcg tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa agggctattg
     6421 cggtaagttg accataattt attcgctcta accacataac gggaagtaat gtgaaatatt
     6481 tactcatttt cttactggtg ttagcgatct tcgtgatttc ggtcacgttg ggtgcgcaga
     6541 acgatcaaca ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt atttccacat
     6601 tgctggcggt attgtttgct gcagggtttg ctatcggttg gttgatttgt ggcctgttct
     6661 ggctgcgagt tcgtgtttcc ctggcgcgcg ctgaacgtaa aataaagcga ctggaaaacc
     6721 agctttcacc cgcgactgac gtggctgtag tgccgcactc gtcagcggcg aaggaataac
     6781 tttctatgct ggagttgttg tttctgcttt tgcctgtagc cgctgcctat ggctggtata
     6841 tgggccgcag aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg tcgcgtgatt
     6901 acgtagcggg ggttaacttc ctgcttagta atcaacagga taaagcggta gacctgtttc
     6961 tcgatatgct taaagaggat acaggcaccg ttgaagccca ccttacgctc ggaaacctgt
     7021 tccgttcgcg tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta atggaaagcg
     7081 cctcgctgac ctatgaacag cgtctgttgg cgattcaaca actagggcgt gattacatgg
     7141 ccgccgggtt atacgaccgc gcggaagaca tgttcaatca gctgaccgat gaaactgact
     7201 tccgcattgg cgctctgcaa cagttgctac aaatctacca ggctaccagt gagtggcaga
     7261 aagcaattga tgttgccgaa cgcctggtga agctgggtaa agataaacag cgcgtcgaaa
     7321 ttgcccattt ctactgtgag ttagccctgc agcatatggc cagcgacgat ctcgatcgtg
     7381 ccatgacctt gctaaaaaaa ggggcggcgg cagataaaaa cagcgcccgc gtatccatca
     7441 tgatgggacg cgtgtttatg gcgaaaggag aatacgccaa agccgtcgaa agtttgcaac
     7501 gcgtgatatc ccaggacaga gaactggtca gcgaaacgct ggaaatgttg caaacctgct
     7561 atcagcagtt gggtaaaact gccgaatggg cagaattcct gcaacgcgcg gtggaagaga
     7621 acaccggtgc cgatgctgaa ttgatgcttg ctgatatcat cgaagcgcgc gacggtagtg
     7681 aggccgcaca ggtctatatt acgcgccagc ttcagcgtca tccgaccatg cgtgtgttcc
     7741 ataagttaat ggattaccac ttaaatgaag cggaagaagg gcgtgccaaa gagagcctga
     7801 tggtgctgcg tgacatggtt ggcgaaaagg tacgtagtaa gcctcgttat cgctgccaga
     7861 aatgtggttt taccgcatac accctctact ggcattgtcc gtcttgtcgg gcctggtcaa
     7921 ccattaaacc gattcgcggt cttgatggcc tgtaattttt aaaaaaatcc tactttagtt
     7981 acaacatact agttattgat gttccattgt gctccggcaa cgacggcgca gaaaaagcct
     8041 gccaggggaa aaatcgcaac tgttaatttt ttatttccac gggtagaatg ctcgccgttt
     8101 acctgtttcg cgccacttcc ggtgcccatc atcaagaagg tctggtcatg acgttaactg
     8161 cttcatcttc ttcccgcgct gttacgaatt ctcctgtggt tgttgccctt gattatcata
     8221 atcgtgatga cgcgctgtcc tttgtcgaca agatcgaccc acgcgattgt cgtctgaagg
     8281 tcggcaaaga gatgtttaca ttgtttgggc cacagtttgt gcgcgaactt caacagcgtg
     8341 gttttgatat ctttcttgac ctgaaattcc acgatattcc taacactgct gcgcacgctg
     8401 tcgctgctgc agctgactta ggcgtgtgga tggtgaatgt tcatgcctct ggtggggcgc
     8461 gtatgatgac cgcagcgcgt gaggcactgg ttccgtttgg caaagatgca ccgcttttga
     8521 ttgctgtgac agtgttgacc agcatggaag ccagcgacct ggtcgatctt ggcatgacac
     8581 tgtcacctgc agattatgca gaacgtctgg cggcactgac gcaaaaatgt ggccttgatg
     8641 gtgtggtgtg ttctgctcag gaagctgtgc gctttaaaca ggtattcggt caggagttca
     8701 aactggttac gccgggcatt cgtccgcagg ggagtgaagc tggtgaccag cgccgcatta
     8761 tgacgccaga acaggcgttg tcggctggtg ttgattatat ggtgattggt cgcccggtaa
     8821 cgcaatcggt agatccagcg cagacgctga aagcgatcaa cgcctcttta cagcggagtg
     8881 catgatgagt gattccaaca gccgtctggt ctactcaacg gaaaccggac gtattgatga
     8941 gcccaaagcg gcccctgtac gccctaaagg cgacggtgtg gtgcgtattc agcgtcagac
     9001 cagtggacgt aaaggtaagg gcgtttgcct gattaccggt gtcgatctcg atgatgccga
     9061 actgacaaaa cttgcagcgg aactgaagaa aaaatgcggc tgcggcggag cagttaaaga
     9121 tggagttatt gaaatccagg gcgataagcg tgatttatta aaatcactac ttgaagcgaa
     9181 agggatgaag gtaaaactcg caggcggtta acataaaaag ccacggatat atccgtggct
     9241 ttcgaatatt ttactgtgcg tattattcag ttatttcgta ctgaacagaa tcaagcgtat
     9301 tataattgcg agtcgataat cgacatttat ttacgattat ttaccgacct ggtgaccaat
     9361 aacaccaccg acagctgcac cacctaatgt acccaacgta ctgccatcgg tcagtactgc
     9421 accgcctaat gcccctgcac ccgcgccgat tgcggtgttg cggtcccgtt tagaccagtt
     9481 agaacaggca ctcagagaca ttgccaaagt aattgccaga acagccgcgg tcattttttt
     9541 gctcgttaca aacataatac tctctcctga atttatga
//
LOCUS       DAPHJE010000149         9425 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPHJE010000149 DAPHJE010000000
VERSION     DAPHJE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.150"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            280..546
                     /locus_tag="RH222_003918"
     CDS             280..546
                     /locus_tag="RH222_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3798864.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            543..1133
                     /locus_tag="RH222_003919"
     CDS             543..1133
                     /locus_tag="RH222_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDV3798865.1"
                     /translation="MMMPVLQKVPFSGLPFSGICGYSFSAAAKSAAGRENPCNSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GILTPVWAIAIERENSGDSSYLLLEGGCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            1126..1413
                     /locus_tag="RH222_003920"
     CDS             1126..1413
                     /locus_tag="RH222_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HDV3798866.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            1406..1861
                     /locus_tag="RH222_003921"
     CDS             1406..1861
                     /locus_tag="RH222_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798867.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILDDGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPASRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            1997..2317
                     /locus_tag="RH222_003922"
     CDS             1997..2317
                     /locus_tag="RH222_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HDV3798868.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            2332..4665
                     /locus_tag="RH222_003923"
     CDS             2332..4665
                     /locus_tag="RH222_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV3798869.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGCLPPADPAVTAAA
                     GAETEAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKYPACQIVLAADRDLSGDGQTKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVD
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            complement(5339..8594)
                     /locus_tag="RH222_003924"
                     /pseudo
     CDS             complement(5339..8594)
                     /locus_tag="RH222_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(8659..8778)
                     /locus_tag="RH222_003925"
     CDS             complement(8659..8778)
                     /locus_tag="RH222_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV3798870.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(8933..9151)
                     /locus_tag="RH222_003926"
     CDS             complement(8933..9151)
                     /locus_tag="RH222_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3798871.1"
                     /translation="MKKLLRTLSAVDARVVCKPNRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
BASE COUNT         2207 a         2605 c         2284 g         2329 t
ORIGIN      
        1 cgctcatgga ctttttgtgc tgtttactac tgtttgtttt tattaattcg ccgggaatga
       61 ataagaagaa acgatttgta tttcactata aaaattacaa attgctttaa gtggctttaa
      121 atgactttaa gtggaaatga acaaaaaaca cacaaccatt gtaaggctgc cggaacaaat
      181 cccccctgtt gcgtctgctg aaaatattca caaaataaag cgctacccga agccggacgg
      241 acttatccgg tgctgtatgg acattaacga ggtagcccga tgcaagctgt tttttcttcc
      301 ccgtctcccg cccctgtgac gccactgatg ccgctgccgg acatcacaca ggagcgtttt
      361 ttacgtcttc cggaagtgat gcacctgtgc ggcctgtcac gctcgaccat ctatgaactc
      421 atccgtaagg gggaatttcc gccgcaggtg agtcttggcg gtaaaaatgt ggcctggctg
      481 cactctgaag tcaccgcatg gatggccggc cgcattgccg gacgcaaacg ggggtacgac
      541 gcatgatgat gcctgttctg caaaaagtcc ctttttctgg cttgcctttt tccggcattt
      601 gcggatatag tttttccgct gccgcaaaat cggcagccgg gcgtgagaac ccgtgtaact
      661 cgaaggcgac atatgacgcg ccatgcgtct ttttttgtgt cgcaatcaac gccacagagc
      721 gccagattat ggtgtggcgt gtggtttgcc gtgcaggtat gatcctgttc gcaatcgcat
      781 gttatgccac tgagtcaatg gtagctcagg cggggcagcc tccgggctgg ccggtattct
      841 tcgaggccgg tattctcacc cccgtctggg ctatcgccat cgagcgtgag aactccggcg
      901 atagcagtta tttgctactc gaaggaggct gccttatggc tacaaccctt accccctcac
      961 accctgaatt tgtctttgtg tttgcggctg tccgtcgcgc agaccgtcat ccccgtatct
     1021 gcatgcttcg caccgtcgcc ggtgatgaac gcagcgcccg ccgttccctt gtccgtgact
     1081 atgtgctctc ccttgctgcc cgtctgccgg tggtggaggt gtcccgtgcg taataaaaaa
     1141 gcccctcaga ccgtctcagc acgtcatgac gcccgtgaac acctcagcat tgaggcttac
     1201 cataagctca accgcgccag cgccgtatcc cagtttgttg ggggtgattt gattcaccgt
     1261 gaactctccg gcctgcatca gctctacatt ccgcatattt tcagctacct gaatgaagat
     1321 attgattttg tgctgaatga gctgaaagcc aaaggcctgt gccgcgattt tctcgcccag
     1381 cagaaagacc ggggagacag gacgcatgtt tgattttccc cagcccggtg agatttaccg
     1441 ttctgccggt tttcccgatg tggccgtggt cggcattctg gaagacggta ttccgtggga
     1501 aatgccgtac cgctgcccgg acattgtctg gaacccgtac cgccgtaaat tcagtatcct
     1561 tgtgcgtatc ctcgatgacg ggcgcaccac agacatcccg ctggggcgtt ttctgcggga
     1621 atttacctgt gaccgtcctg acctgtttaa acgcagcccc gtaaaccggc atgcggtact
     1681 gaaagaaatg gccggagacc cggaattaca gaaatggcgg gagaaatatc tggatattta
     1741 cccgcaggac actgttccgg ccagccgggc ggcaccggtg gcgcgggaat ggcgggaaat
     1801 tccccgcacg gagcctgacc cggaaatcac cccggataac agttaccgca attatctgta
     1861 attaaaaaac gatacccgaa aaattaaatg tgcgtattcg cgcagggata cgcacgtctt
     1921 caggagacgc agatatgcct tatcagttaa tgcaaccggc acggaatgca gtcatctgtc
     1981 acagggagga aaacaaatga aaacaccctt accgcccgtc ttacgcgctg ccctttaccg
     2041 tcgcgctgtc gcctgtgcct ggctgaccgt gtgcgaacgt cagcaccgct acccgcatct
     2101 cacccttgag tcactggagg cggccatcgc cgctgagctg gaaggctttt atctgcgcca
     2161 gcacggtgag gaaaaagggc gtcagatagc ctgtgccctg ctggaagatt taatggaatc
     2221 cggccccctg aaggccgcgc cgtcgctgtc ctttctcggg ctggttgtga tggatgaact
     2281 ctgtgcccgt cacataaaag cgccggtact gcactgaagg agaacaacac catgaaaatg
     2341 aacgtaaccg ccaccgtcag ccatgcgctc ggccactggc cgcgtattct cccggcgctg
     2401 gggattcagg tgctgaagaa ccgtcatcag ccctgtccgg tctgtggcgg gagtgaccgc
     2461 ttccgttttg atgacaggga ggggcgtggc acctggtact gcaatcagtg tggtgccggt
     2521 gacggcctga aactggttga aaaggtgttt ggtgtttccc cgtccgacgc ggccgcaaag
     2581 gtggctgccg tgaccggctg cctgcccccg gctgacccgg cagtgacggc cgccgccggt
     2641 gctgaaacag aagccgcccg gaagaacgcc gccgcactgg cacaaaccct gatggcaaaa
     2701 acccgtcccg gaaccggtaa cgcctacctg acccgcaagg gctttcccgg ccgggaatgc
     2761 cggatgctga ccggcacaca cagagccggt ggcgtgagct ggcgcgccgg tgaccttgtg
     2821 gtgccactgt atgacgacag cggcgaactg gttaaccttc agttaatcag tgctgacggc
     2881 cgtaagcgca ccctgaaagg cggacaggtc aggggcacct gtcacaccct tgaaggacag
     2941 aatcaggcag gaaaacgtct gtggatagcg gagggatacg cgaccgcact taccgtgcat
     3001 cacctgaccg gtgaaacggt gatggtggcg ctttcttccg tgaacctcct ttctctggcc
     3061 agccttgccc gacagaagta tccggcctgt cagattgtcc ttgccgcaga ccgtgacctc
     3121 agcggtgacg gccagacaaa agccgccgca gccgcagatg cgtgtgaggg cgttgttgcc
     3181 ctgccgccgg tcttcggtga ctggaatgat gccttcacgc agtacggcgg ggaggccacc
     3241 cgtaaggcca tttacgatgc catccggcca ccggctgaaa gcccgttcga caccatgagc
     3301 gaagcggagt tttccgccat gagtaccagc gaaaaggcca tgcgtatcta tgagcattac
     3361 ggcgaggcgc tcgcggttga tgccaacggc cagcttctgt cccgctatga aaatggtgtc
     3421 tggaaggtgc tgccaccaca ggactttgcc cgggatgtgg ccgggctgtt tcagcgtctg
     3481 cgcgcgccgt tctcctccgg gaaggtggcc tccgtggtgg acaccctgaa gctgattatt
     3541 ccgcagcagg aagccccctc ccgccgcctg attggctttc gtaacggcgt gctcgacacg
     3601 cagaacggca cgttccaccc gcacagtccg tcacactgga tgcgcaccct gtgcgatgtg
     3661 gatttcaccc cgccggtgga cggtgaaaca ctggagaccc acgcccccgc gttctggcgc
     3721 tggcttgacc gtgccgccgg tggccgtgcg gaaaaacgcg acgtgattct ggccgcactg
     3781 tttatggtgc tggcaaaccg ctacgactgg cagctctttc tggaggtgac cggtcccggc
     3841 ggcagcggca aaagtatcat ggccgaaata gccaccctgc tggctggtga ggataacgcc
     3901 acgtcggcca ccatcgagac gctggaatcc ccgcgtgaac gtgccgcgtt aactggcttc
     3961 tcactgatac gcctgccgga ccaggaaaaa tggagcggcg acggagccgg actcaaggcc
     4021 atcaccggcg gcgatgcggt gtccgttgac ccgaaatacc gggatgcgta ctccacgcac
     4081 atcccggcgg taattctggc cgtgaacaat aacccgatgc gcttcaccga ccgcagcggc
     4141 ggcgtgtcac gccggcgggt gattattcac ttcccggaac agatagcccc gcaggagcgc
     4201 gacccgcagc ttaaggacaa aatcacccgc gagctggcgg tcatcgtgcg tcacctgatg
     4261 cagaagttca gcgacccgat gctcgcccgg tcactgcttc agtcccagca gaactcagac
     4321 gaggcactga acatcaaacg ggatgccgac ccgacgtttg attttatcgg ctatctggaa
     4381 accctgccgc agaccagcgg catgtatatg gggaacgcca gtatcatccc gcgtaattac
     4441 cgtaaatacc tctatcacgc ctatctggcc tacatggagg caaacggcta ccggaatgta
     4501 ctcagtctga aaatgttcgg gctggggctg ccggtgatgc tgaaggaata cggactgaat
     4561 tacgagaagc gccataccaa acaggggata cagaccaacc tgacgctgaa agaggaaagc
     4621 tacggcgact ggctgccaaa atgtgacgac cctgcaacaa cctgaccatc atgactcatc
     4681 tgaccggcat ctgccggtct tttttcatcc ctgaatcccc cgaaggtgaa caatccagtg
     4741 ttcacccttc accgtatgtt cacccgttat cacactgaaa tcaaaagaga aaaacgaaag
     4801 gtgaacagtg tgaacaatca aatcaaaaaa aacttttttt cttcctgtgt gattttcagt
     4861 gcggaggatt aatcgccggt atgagtcaca ccggcagaat gccggaggtg aagaatcgaa
     4921 tgttcaccct tcacccatta ttcaccacct atccaactga aataaaagga aaaaacagaa
     4981 aggtgaacag tgtgaacagt tcttccgaaa aaaaattttt ttcctgtacg atatagcatc
     5041 aatccaagta aaaatgcgct ttcatttcac attaacttac agccattggt atacgattag
     5101 gtatacgcac aaaaactgaa ttaaacaaaa tcattaaaaa tcaaagcaat acaatattta
     5161 ttcaaactcc gccagccaat catgattgga cggtataagg acaacaccaa taaaaacagg
     5221 aagttagcag tctcagcagg acaccgacca gacggtgaag agacataaaa ggatacgcaa
     5281 aggagccgcg gctcctggtg acatgaaagc ccacagatgt gggctttttc gttgatggtc
     5341 agaacgacca gttcacacca gctaccccgt tccacgggga ttccacaccg gcaccatggc
     5401 tataccccac cccaagatgc ccgcttaacg tactgctgaa tgaggcttta atacctgcct
     5461 ggtatattcc acgtctgccc gacaaatcat tgacgaaatt accgtcacta ttcactttca
     5521 cccggttatc atcgacaaat tctttgcgca cagccgcctt cagccacggc tcaacttcca
     5581 taccgttccc cagacgcatg ttgtaactca gcgttgcacc cagttcacga tataaactgc
     5641 gggtatcgac tgatttcgat tccatgccat tggataaatg atattcaggg ttatcagcgg
     5701 tgaacccccg ttaacgatgc atacggcgtc aggttccagt taccatcggt aaatcgcatc
     5761 ccggtttcaa tgtgaccgcc cagcccgtag ctgcgataac tgccgttggc ggctccaccg
     5821 ctgccaaccg tggagtcgcg agcttcggta cctgctaata ccaataaatt accgccattt
     5881 tccagcaaca tattggtcgc taaattgccg gaaatggaaa aagtgccgta ctggtgagta
     5941 ccgctgattt caataccgtt agccgtgctc gtctggagag cggcaccgct gttctggacg
     6001 atatctgtcg ctttgccatt atcgttaact gtcagcgtac cgccttcatt gatctttgtt
     6061 tttattgcct ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat cgttgtctca
     6121 ttcgccaccc cctcgacgat ttgttcgccg ccggtgagcg tcgtgcctgt cgcagtggct
     6181 tttgttttga cgatctcccg tccgcccgta ttgacctgtg ttttgtcaga agaggtgtct
     6241 gactccacgg ttaacacgcc gccatttgcc agcaggatat tgttcgccgc accctgctcg
     6301 atgctgaacg cgacgccatc cgcgcgtgtt cctgtgaccc gcgtcgccct ggtggttgca
     6361 accaaagcgc cctggctact ctgctgtatc cccgttgcgc tgcctttctc ccgcacatcg
     6421 agtgtgccgc cgtcattaag caccgagttt tcagccagac cgccctcatt aactacctgt
     6481 gaacacccat taataatgga accttccgct atcccgtttg ccataatttg ttgtaggcca
     6541 gagacgatat cggtattgat cgccttacca taattctgaa cggtttgtgt gccgccattg
     6601 atgtgcgttt tgtcagttga cccaccatca acaatttgtt caccactttc aatatttgcc
     6661 tcagtggcta aaccatatac cgtttgcttg ccacctttga tatttgcttt atcagaagtg
     6721 gcactggcat atattgtttg ggtgccagca ctattaagta cagtgccaac atcttttcca
     6781 taaacatcca ttttgccgtt ggcattaata atcgtatcaa ctgcccggga accagtgacg
     6841 actgttaatg agccagcgtt ttccagcact acatttttag cttctgaatt cctgatgtag
     6901 aaagcatcac cataactgtt ggttccttcg ataagggttc cggaagttgt ggaagcaatt
     6961 aatgcgccgc cggattgttg ctcaacatgc ttagcctcac caccgtcctg aacctccaga
     7021 acgccaccat tattaagtct ggtggtatct gttttagcct ccttctggac aatcagctta
     7081 ccgccagcat caacggtagt atttttcgcc gaggttttag ccactaccgt cagttcgccg
     7141 gtattttcca gcacaacata attagcctcc cctccggtaa tagtgaagtg agagagcttg
     7201 ttgtatcctt caatatcagt ccctgcgccc gtgttggcaa ctaaagcact gcccgtctcc
     7261 tggttaaccc catgtgcaat accgccggta tagacaatca gcgagcctcc ggcgctaata
     7321 ttgctgccaa ttgccgtacc atctttctca acaacttgct ggctcccgga ggatataatt
     7381 gttgtgtcag ctttcccacc gcttttgata ttttgcgttc cgctgttgat attggtgcct
     7441 gtggctatgc cataattatt aagattctgt gtgccaccat taattatggt atttgtcgcg
     7501 tttcctgcaa catccataac cccgccatta tctattcggg tcgcatcagc tttagcattc
     7561 gttaaaactg acattgttcc tttatcttta ataatcgtct tgtttgcgga atgagatact
     7621 tctaaatggc ctccattttc cagcaacaca ttgtcttcca ctttattgtg gatggagaat
     7681 gcaccttcac tattcgtacc gctcaccgtc gtaccgttag tgtttgtttt taaaattgta
     7741 ccatcgtgct gggtaacatt tgttgccgta ccaccactaa catcaagcac gccaccggaa
     7801 taaacttcaa taacatccga ggtgctggtg ttatcaacaa tttgcgtgcc accagaatag
     7861 atctgcgtaa tttttgccgt tgacttacta ttcagagact gagttccgcc ttcaatcgtc
     7921 gtgtccagcg cacggctctc atatactctt tgctcaccgc catttttaat ggttgttgtt
     7981 tctactgtgc tctgttcaac atactgtcga ccaccattta tggttgtgtt cgttgccaga
     8041 cttccttgta ctacgtcctg agagccagac ttatttatcg ttgtaccatc agcatgccct
     8101 tgaactttta ctatctggct accaccatca atgagtattc cattcgcact ccctccctct
     8161 acgcgtgaag caccgccctt aattgtcgtt ccattgctga tacccccttt ataaacgtcc
     8221 tgattgccac tctcgattgt cgtacctgtg gaaatacccc cgtcatgaat tgactgtctg
     8281 ccaccgttaa tggttgtatt attagcctgc cccacaaaat tgttatgact tcctatatct
     8341 tgatatccac cagattcaat aagacttcca ttagataccc cgccatgaac attctgctgg
     8401 ccatggttga taatatgagt gttatttgtt gtacctcgtt catccacttt ttggttgcca
     8461 tctacagtct catcgtttac cacaccaata acatcaggag tgaaggccgc cataccgggc
     8521 ggggcatata tcaaggcaga tatcaataag gaaagtactg agcggcgaca ataatgggga
     8581 ctggtcctgt tcataaattt catcctctga aaagtgaata ctgagtagcg tttaagcgac
     8641 cttagctctg ctgcaacatc agcccacagg caccagacca ggggattcat cctgaagaga
     8701 cagcgcaagt gtattgtgtt caccgctcat caaagacatc atgatgaaat gatgatattc
     8761 cacataagaa agaagcattt tttaaacgca gtgcgctgaa gtatggttgg ataaaaaagt
     8821 caatctattc agggaatacg ggggtattct tttctttcga caatcaggtc gtcggcaaaa
     8881 taaaatgatt tacataatcg tttctgatga atatcttctg ctcacataaa aatcacacaa
     8941 taactttgag atcgcagatt cttttacttt tacagcattc gtccccccat tgttgggcaa
     9001 atatagattg ggccagagca cgaaagttaa taccacgttt gcacagctcc tccaacagca
     9061 cgacaagatg ccacatactg cgccccagtc ggttcggttt acagactacc cgtgcgtcca
     9121 ccgccgataa tgtcctgagc agttttttca gtccggacct cctgccacgg ttttacgttg
     9181 aagaggtaac cctgagcacg cagttcatca gtcaggcgtg gtgtaccgta accctattat
     9241 tgatgggtaa gatcaaaaaa actttcaggc agctaaggaa agttgaacca gacattagaa
     9301 taaatatttc aaccaattac agcaccaatt cagacaccgc cagctcaaca aataaatcaa
     9361 ggggttacgt gaaagcgtag cccctttttc tttggtagtg gcagcaaaat ggttgtagtg
     9421 taaaa
//
LOCUS       DAPHJE010000150         9108 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPHJE010000150 DAPHJE010000000
VERSION     DAPHJE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.152"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..999
                     /locus_tag="RH222_003927"
                     /pseudo
     CDS             1..999
                     /locus_tag="RH222_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            complement(1177..1458)
                     /locus_tag="RH222_003928"
     CDS             complement(1177..1458)
                     /locus_tag="RH222_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798872.1"
                     /translation="MSESVKLSFYRVHECGYYLWGNNTPVFGSLQELLTDLHFWSTDK
                     SIENTKLYEPQADSDYLGTYLFNINRLGGYWLVTIWNEVPSTKRELLLL"
     gene            complement(1644..7154)
                     /locus_tag="RH222_003929"
     CDS             complement(1644..7154)
                     /locus_tag="RH222_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3798873.1"
                     /translation="MINKNKVTERSGIHDTVKSLSENLRKYIEAQYHIRDEGLIAERR
                     ALLQQNETIAQAPYIEATPIYEPGAPYSELPIPEAAINVLTQLSELGIGLYQRPYKHQ
                     SQALESFLGEDASDLVIATGTGSGKTESFLMPIIGKLAIESSERPKSASLPGCRAILL
                     YPMNALVNDQLARIRRLFGDSEASKILRSGRCAPVRFGAYTGRTPYPGRRSSRRDELF
                     IKPLFDEFYNKLANNAPVRAELNRIGRWPSKDLDAFYGQSASQAKTYVSGKKTGKQFV
                     LNNWGERLITQPEDRELMTRHEMQNRCPELLITNYSMLEYMLMRPIERNIFEQTKEWL
                     KADEMNELILVLDEAHMYRGAGGAEVALLIRRLCARLDIPRERMRCILTSASLGSIED
                     GERFAQDLTGLSPTSSRKFRIIEGTRESRPESQIVTSKEANALAEFDLNSFQCVAEDL
                     ESAYAAIESLAERMGWQKPMIKDHSTLRNWLFDNLTGFGPIETLIEIVSGKAVKLNIL
                     SENLFPDSPQQIAERATDALLALGCYAQRASDGRVLIPTRMHLFYRGLPGLYACIDPD
                     CNQRLGNHSGPTILGRLYTKPLDQCKCASKGRVYELFTHRDCGAAFIRGYVSSEMDFV
                     WHQPNGPLSEDEDIDLVPIDILVEETPHVHSDYQDRWLHIATGRLSKQCQDEDSGYRK
                     VFIPDRVKSGSEITFDECPVCMRKTRSAQNEPSKIMDHVTKGEAPFTTLVRTQISHQP
                     ASRPIDGKHPNGGKKVLIFSDGRQKAARLARDIPRDIELDLFRQSIALACSKLKDINR
                     EPKPTSVLYLAFLSVLSEHDLLIFDGEDSRKVVMARDEFYRDYNSDLAQAFDDNFSPQ
                     ESPSRYKIALLKLLCSNYYSLSGTTVGFVEPSQLKSKKMWEDVQSKKLNIESKDVHAL
                     AVAWIDTLLTEFAFDESIDSTLRIKAAGFYKPTWGSQGRFGKALRKTLIQHPAMGELY
                     VEVLEEIFRTHLTLGKDGVYFLAPNALRLKIDLLHVWKQCNDCTALMPFALEHSTCLA
                     CGSNSVKTVEPSESSYINARKGFWRSPVEEVLVSNSRLLNLSVEEHTAQLSHRDRASV
                     HATTELYELRFQDVLINDNDKPIDVLSCTTTMEVGVDIGSLVAVALRNVPPQRENYQQ
                     RAGRAGRRGASVSTVVTYSQNGPHDSYYFLNPERIVAGSPRTPEVKVNNPKIARRHVH
                     SFLVQTFFHELMEQGIYNPTEKTAILEKALGTTRDFFHGAKDTGLNLDSFNNWVKNRI
                     LSTNGDLRTSVAAWLPPVLETGGLSASDWFAKVAEEFLNTLHGLAEIVPQIAVLVDEE
                     NEDDEQTSGGMKFAQEELLEFLFYHGLLPSYAFPTSLCSFLVEKIVKNIRGSFEVRTV
                     QQPQQSISQALSEYAPGRLIVIDRKTYRSGGVFSNALKGELNRARKLFNNPKKFIHCD
                     KCSFVRDPHNNQNSENTCPICGGILKVEIMIQPEVFGPENAKELNEDDREQEITYVTA
                     AQYPQPVDPEDFKFNNGGAHIVFTHAIDQKLVTVNRGKNEGESSGFSVCCECGAASVY
                     DSYSPAKGAHERPYKYIATKETPRLCSGEYKRVFLGHDFRTDLLLLRITVGSPLVTDT
                     SNAIVLRMYEDALYTIAEALRLAASRHKQLDLDPAEFGSGFRILPTIEEDTQALDLFL
                     YDTLSGGAGYAEVAAANLDDILTATLALLESCECDTSCTDCLNHFHNQHIQSRLDRKL
                     GASLLRYALYGMVPRCASPDIQVEKLSQLRASLELDGFQCIIKGTQEAPMIVSLNDRS
                     VAVGSYPGLIDRPDFQHDVYKSKHTNAHIAFNEYLLRSNLPQSHQNIRKMLR"
     gene            complement(7151..8194)
                     /locus_tag="RH222_003930"
     CDS             complement(7151..8194)
                     /locus_tag="RH222_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798874.1"
                     /translation="MEWRAVSRDKALDMLSTALNCRFDDEGLRISAVSECLRSVLYQY
                     SISETEEARQTVTSLRLTSAVRRKLVPLWPDIADIDNAIHPGIMSILNSLAELGDMIK
                     LEGGNWLTAPPHAVRIDNKMAVFFGGEPSCTFSTGVVAKSAGRVRLVEEKVCTGSVEI
                     WDANEWIGAPAEGNEEWSSRLLSGTISGFIDAPSNMSETTAYVRGKWLHLSELSFNKK
                     QIYLCRMSVDNHFSYYLGEIEAGRLCRMNSLESSDDVRRLRFFLDTKDNCPLKIRIKI
                     SNGLARLRLTRRLPRRETKVLLLGWRESGFENEHSGITHHVFPEEILPIVRSAFEGLG
                     IIWINEFTRRNEI"
     gene            complement(8206..>9108)
                     /locus_tag="RH222_003931"
     CDS             complement(8206..>9108)
                     /locus_tag="RH222_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798875.1"
                     /translation="ASKVSVTNRFTKFHEIAHYENYEYLSSSEDISNRISLNLQAVGL
                     TKNSAETLARLTLATFVSGQIIQFSGSLADIIADAIAIAIGAPRYHIWRVPVGIISDM
                     DSFDFIETIAESSRCLLLKGANLSAFEIYGAAIRDIVVQRQIHPTNYDHLALIATWKQ
                     GPATFPDGGMLAELGPVIDTDTLKMRGLSAILPQLKPGCLAKDKWTNIDGLQLDSVDD
                     YVDELRALLDEAGFDGGTLWKRMVHIFYTSLIRVPNGNYIYDLYSVLSFYTLTWAKIK
                     GGPVQKIEDIANRELKNYSAKISS"
BASE COUNT         2621 a         2043 c         1841 g         2603 t
ORIGIN      
        1 tcagcagacg tcttctgaac gtgaactgga gatgtctacc gagattgcac gtctcaaacg
       61 ccagctggca gaacgggatg aagagctggc tatcctccaa aaggccgcga catacttcgc
      121 gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc aggctgagtt cagcatcaaa
      181 gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt atacgtggtg tcagcggcgg
      241 acaaggataa gcacgcgtca gcagttccgc caacactgcg acagcgttgt cctcgcggct
      301 tttacccggt caaaacagcg ttacggtgcc ccacgcctga cggatgaact gcgtgctcag
      361 ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc gccgtcaggg actgagggca
      421 aaggcctccc ggaagttcag cccggtcagc taccgcgcac acggcctgcc tgtgtcagaa
      481 aatctgttgg agcaggattt ttacgccagt ggcccgaacc agaagtgggc aggagacatc
      541 acgtacttac gtacagatga aggctggctg tatctggcag tggtcattga cctgtggtca
      601 cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg cgcaactggc ctgcgatgcc
      661 ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg ttatcgttca cacggaccgt
      721 ggaggccagt actgttcagc agattatcag gcgcaactga agcggcataa tctgcgtgga
      781 agtatgagcg caaaaggttg ctgctacgat aatgcctgcg tggaaagctt ctttcattca
      841 ctgaaagtgg aatgtctcca tggagaacac tttatcagcc gggaaataat gcgggcaacg
      901 gtgtttaatt atatcgaatg tgattacaat cgctggcgac ggcacagttg gtgtggcggt
      961 ctcagtccgg aacaatttga aaacaagaac ctcgcttaga gcagtgtcca tattacgagg
     1021 gtaggatcag ttgttctgtt tgagttctat ttttgaatcg cacactggca aataatccat
     1081 aactaggtaa aatccaaaaa taagtcgcgt aaccgggaat agagttacct tgtatttggt
     1141 tcataactac atcgggcgca gtaacacttg aatgcctcat aacagaagca actcccgctt
     1201 ggttgatgga acttcattcc aaatagtaac tagccaataa ccgcctaacc tgttgatatt
     1261 aaataagtaa gtacctaaat aatcactatc tgcttgtggt tcataaagtt ttgtgttttc
     1321 tatactctta tccgtactcc aaaagtgaag atctgtcagg agctcctgaa gtgagccaaa
     1381 aacaggagta ttattacccc ataagtagta tccacattca tgtactcgat aaaaactaag
     1441 tttaaccgat tcgctcattg gtcagaagtt cccttacctt attattctca atgggcataa
     1501 ttttacattg tgcaatagtt gctggcgagt taaatggtca cattttctcc catacgtaca
     1561 tttatggctt cagttaaagc ccgctcacgc actgaaaacc ttgagtgccc tacagggctt
     1621 tagggcactc aatactgctg ctatcagcgc aacatttttc taatattttg atgcgattgt
     1681 ggcagatttg aacgaagaag atattcatta aaggctatat gagcattagt atgctttgac
     1741 ttatatacgt cgtgttgaaa gtcgggtcga tcaataagac caggataact tcccactgca
     1801 acagaacggt cattcaaact cacaatcata ggtgcctcct gagttccctt aattatgcat
     1861 tgaaaaccat ccaattccag acttgccctc aattgagaca atttttctac ctgaatatca
     1921 ggtgaagcac aacgaggaac cattccgtat agtgcataac gaagtaaaga tgcacctagt
     1981 ttcctatcga gacggctttg tatatgctgg ttgtggaaat gattgagaca atctgtacag
     2041 gaggtatcgc actcacagct ttctaacaat gcgagtgttg cagtaagaat gtcatctaga
     2101 ttcgctgctg ctacttccgc ataacccgca ccgccggata aagtatcata aaggaagaga
     2161 tccaatgcct gagtatcttc ctctatagtg ggtaaaattc tgaaaccaga gccaaactca
     2221 gcaggatcaa gatccagttg tttatggcga ctagctgcaa gccttagtgc ttccgctatt
     2281 gtatataatg catcttcata catccgtaaa acgatagcat ttgaagtatc agttacaagc
     2341 ggagacccaa cggttattcg taataaaagc aaatcagtac ggaaatcatg tccgagaaaa
     2401 acgcgtttat actcgccaga gcataagcga ggcgtttcct tagttgctat atatttatac
     2461 ggtctttcat gtgccccctt tgccggtgag taggaatcat aaacggaggc cgcaccacat
     2521 tcgcaacata ctgaaaaacc actggactcc ccctcatttt tccctcggtt caccgtcacc
     2581 agtttctgat ctattgcgtg agtaaaaaca atatgagcac ctccattatt gaacttaaaa
     2641 tcttcaggat caacaggttg tggatattgt gccgctgtta cataggtgat ttcttgctct
     2701 ctgtcgtcct cattaagttc cttggcattt tcaggtccaa agacttcggg ctgaatcatt
     2761 atttctactt ttagaatgcc accacagatc ggacaagtat tttcgctatt ctgattatta
     2821 tgaggatcgc ggacaaaaga gcacttatcg caatgaataa actttttggg attattgaaa
     2881 agctttcttg cccggtttag ttcgcctttc aatgcattag aaaaaacacc accagagcga
     2941 taggttttcc tatcaataac aatcaaacgt cccggggcat attcactcag agcctgagaa
     3001 attgattgct gaggctgttg tactgttcgc acctcaaaag aacctctaat attctttaca
     3061 attttttcta ccaagaaact acagaggctt gtaggaaatg cataacttgg taataaacca
     3121 tggtaaaaca ggaactcaag taattcttct tgtgcaaatt tcattccacc agaagtctgc
     3181 tcatcatctt cattttcctc atcaacaaga acggcaattt gtggaacaat ttcagccagc
     3241 ccatggagtg tatttaaaaa ttcctctgct accttagcaa accagtcact ggcagaaagc
     3301 cctccagttt caagaacagg aggaagccat gctgcaacac ttgttctcaa atcaccatta
     3361 gtagatagaa tacggttttt aacccaatta ttaaagctat cgagatttag gccagtatct
     3421 tttgctccat gaaaaaaatc tcgtgtagta ccaagtgctt tctcaagtat ggcagttttc
     3481 tctgtgggat tataaattcc ttgttccatt aactcgtgaa aaaaggtctg aactaaaaaa
     3541 gaatgaacgt gtcttctggc tattttggga ttatttactt tcacttcagg tgtacgagga
     3601 gaacctgcaa caatgcgttc aggattaagg aaataataac tatcatgagg gccattttga
     3661 gaatatgtaa ccaccgttga aacagatgcg ccacggcggc ctgctcgccc agcacgttgc
     3721 tgataatttt ctcgttgcgg agggacgttt cttaaagcaa cagcaaccag agatccaata
     3781 tcaaccccca cttccatcgt cgtcgtacaa ctaagtacat caatgggctt gtcgttatca
     3841 ttaataagaa catcttggaa tctcagttcg tagagttctg tagtggcatg aacgctggcc
     3901 ctatctctat gtgagagttg agcagtatgc tcttcaacgc taaggtttag aagccgcgaa
     3961 tttgaaacca aaacttcttc taccggcgaa cgccagaatc cttttcgtgc attaatatag
     4021 ctgctttccg acggctcgac tgttttgaca ctgttactac cacaagcaag gcaagtagaa
     4081 tgttctaaag caaatggcat tagtgccgtg cagtcattac attgtttcca gacatgcaag
     4141 agatctattt tcagacgtag tgcatttgga gcaagaaagt agacaccatc ttttcctaat
     4201 gtcagatgag tacgaaaaat ctcctccaaa acttccacat aaagctcccc catagcagga
     4261 tgctgtatca gggttttcct aagagctttt ccaaaccgtc cttgactacc ccaagtgggt
     4321 ttgtagaatc cagcggcttt gattcgtagt gtcgaatcaa tagattcatc aaaagcaaat
     4381 tcagtgagta aggtatcaat ccaagcaaca gctaaagcat gaacatcctt gctctcaata
     4441 ttgagcttct tggactgcac atcttcccac attttttttg atttaagctg cgatggttca
     4501 acaaaaccaa ctgttgttcc ggaaagagaa tagtaattgc tacataaaag tttaagcaac
     4561 gctattttat atcgtgacgg tgactcttgg ggactgaagt tatcatcaaa agcttgagcc
     4621 agatcgctat tataatcacg ataaaattca tcacgggcca ttacaacttt tcgtgaatct
     4681 tccccatcaa aaataagcaa gtcatgttca gaaaggactg ataggaaagc aaggtaaagt
     4741 actgatgttg gtttgggttc ccgattgata tctttcagtt tagaacaggc gagagcaatg
     4801 gattgccgaa acaaatcaag ctcaatatct ctaggaatat cacgtgcaag ccgagctgct
     4861 ttttgtcggc catcagaaaa aataagtact ttttttcccc cattgggatg tttaccatca
     4921 ataggacgac tcgctggctg gtgagatatc tgtgtacgta ctaacgttgt aaaaggtgct
     4981 tccccttttg taacatgatc cataatttta gacggttcat tctgagcact tcttgtctta
     5041 cgcatacaaa cagggcactc atcaaatgta atttcagatc cagacttaac tcggtcaggt
     5101 ataaagactt tacgataacc agaatcctca tcttgacact gtttagaaag gcgtcctgtt
     5161 gctatatgta gccatctgtc ctggtaatca ctatgtacat gaggtgtttc ctcgaccaat
     5221 atatctatgg gaacaagatc gatatcctca tcttctgata atggtccgtt cggctggtgc
     5281 catacaaagt ccatttcgga actaacgtat ccacgaataa aagccgcacc gcagtcacgg
     5341 tgggtaaata attcgtagac tcgccctttt gaagcgcatt tacattgatc cagtggtttc
     5401 gtataaaggc ggccaagtat agttggccca ctatggttac ccaaacgttg attacaatcg
     5461 ggatctatac aggcataaag acctggtaat ccccgataaa aaagatgcat gcgagttgga
     5521 ataagcactc tgccatcgga tgccctctga gcatagcaac ccaatgcgag taatgcatct
     5581 gtcgctcgct ctgcaatttg ctgtggagag tctggaaaaa ggttttcact caagatattt
     5641 agcttaaccg ctttacctga aactatttca ataagcgttt caataggacc aaaaccagtc
     5701 aaattatcaa ataaccaatt acgtagtgta ctatgatctt ttatcatcgg cttttgccag
     5761 cccattcgtt cggcaagaga ctctattgct gcatatgcag attcaagatc ctcagctacg
     5821 cactgaaatg aatttaggtc gaattcagcc agtgcattag cttctttact ggtaacaatt
     5881 tgtgactcag gacgcgattc ccttgtaccc tcaataattc gaaatttcct cgaagaggtt
     5941 ggtgataagc cagttaagtc ttgggcaaaa cgttctccat cctcaatgga ccctagacta
     6001 gcactggtaa ggatgcagcg catacgttcc cggggaatat ccaaccgagc acagaggcga
     6061 cgtattaaaa gggctacctc tgctccccct gctcctctat acatatgcgc ttcatcaagc
     6121 actaagataa gctcattcat ctcatcagct ttgagccatt ccttagtctg ctcaaaaata
     6181 ttacgctcga taggtcgcat cagcatatac tcaagcatgg agtagttcgt tatcagtaat
     6241 tctggacagc gattctgcat ttcatgccgg gtcattagct cacgatcctc aggctgggta
     6301 attagcctct ccccccaatt gttcaaaaca aattgcttac ccgttttttt gcctgagacg
     6361 taggttttag cctgagatgc gctttgccca taaaaagcat caagatcttt acttggccag
     6421 cgaccaatgc ggttcagttc cgcacgtacg ggggcgttat ttgcgagttt attgtaaaac
     6481 tcatcgaaaa ggggtttgat aaaaagctcg tctcgtctag agctacgacg accagggtaa
     6541 ggcgttcttc ccgtataagc gccaaagcgt acaggggcac atcttccaga tctcagtatt
     6601 ttagaggctt cagaatcacc aaaaagacgt ctgatacgag caagttgatc gttaactaat
     6661 gcattcattg gatataataa aattgctcta caacctggaa gggatgcaga tttaggtctc
     6721 tcggaagatt caatcgccaa tttaccaata attggcatta gaaagctttc agtcttaccg
     6781 gagcctgtac ctgttgcaat gaccagatca gaagcgtctt caccaagaaa tgactcaagt
     6841 gcctgtgatt ggtgtttata ggggcgttga tagaggccaa ttccaagttc tgatagttga
     6901 gttagcacat taattgctgc ttcgggaata ggcaattcac tgtatggcgc accaggttca
     6961 taaattgggg ttgcttctat ataaggagct tgagcaatag tttcattttg ctgtaaaagc
     7021 gctcgtcgct cagcaattaa cccttcatcc cggatatgat attgtgcctc aatgtatttt
     7081 ctcagattct cactaaggct tttcacggta tcatgtatac ctgaacgttc agttacttta
     7141 tttttattaa tcatatttca ttccgtcgcg tgaattcgtt aatccaaata ataccaagcc
     7201 cttcaaaagc gctacgcact atgggtaata tttcctcggg gaatacatgg tgtgttattc
     7261 ctgaatgttc attttcaaaa cctgattctc tccagcctag caggagtacc ttcgtttctc
     7321 gtcttggtaa tcttctggtt aatcttaatc ttgctagccc attagatatt ttgatacgga
     7381 tctttagcgg acaattatct tttgtatcga gaaaaaaacg taatcttctg acatcatcag
     7441 acgattctaa cgaattcatt ctacataagc gtccagcttc aatttctcct aaataatagg
     7501 aaaagtgatt atcaacggac attctgcata agtagatttg ctttttatta aaagaaagtt
     7561 ctgacaaatg gagccatttt ccccgcacat atgcagtcgt ttcactcata ttgctaggcg
     7621 catcgataaa gccggaaata gttccagata gtagtctgga tgaccattct tcattgcctt
     7681 ctgctggggc accaatccac tcatttgcat cccagatttc aacacttcca gtacacactt
     7741 tttcttcaac caagcgaact cttccagcag atttagctac cacgcccgtt gaaaatgtac
     7801 aggaaggctc tccaccaaaa aaaacagcca tcttattgtc aattcgtact gcatgtgggg
     7861 gagctgtgag ccaattacca ccttctaact taatcatgtc acccaattca gccaagctgt
     7921 tcaatataga cataatgccc ggatgtatag cattatcaat atcagcaatg tctggccata
     7981 aaggtaccaa ttttcgcctt actgcactag tgagtcgaag cgaggttaca gtttgcctag
     8041 cttcttctgt ttcagatata gaatattgat ataatacgct ccttaagcat tctgaaactg
     8101 ctgaaattct caacccttca tcatcaaatc gacaatttaa tgcagttgat aacatatcca
     8161 gtgctttgtc tcgtgatact gctctccact ccattaacca cctcctcaag aagatatttt
     8221 tgcactataa ttttttaatt cacgattggc aatatcttct atcttttgga cggggccacc
     8281 tttaattttt gcccatgtaa gagtataaaa agacaagaca gaataaagat cataaatata
     8341 atttccatta gggactctta tgagtgaagt atagaaaata tgaaccattc tcttccacaa
     8401 agttccccca tcaaatccag cttcgtccag taatgctctt aattcatcta cataatcatc
     8461 aacactatca agctgtagtc catcaatatt tgtccattta tccttggcaa gacaacctgg
     8521 tttcaattgg ggtaaaatag cagataaacc acgcattttt aatgtatcag tatcaataac
     8581 tggtcccaac tcggccaaca ttcctccatc agggaatgta gcagggcctt gtttccaggt
     8641 agctatcaat gccagatggt cataatttgt tggatgtatt tgccgttgaa caactatatc
     8701 tctaattgcc gctccataaa tctcaaatgc tgaaagattg gcccctttca aaaggagaca
     8761 gcgagatgat tcagctatag tctctatgaa atcaaaagaa tccatgtcag aaataatacc
     8821 aactggaact ctccatatgt gataacgtgg tgcaccaata gcaatggcaa ttgcatccgc
     8881 gataatatct gccaaagagc cactgaattg aatgatttgt ccagaaacga aggtagctaa
     8941 tgtcaatcta gccaatgttt ctgctgaatt ttttgttaac ccaacagcct gtaaatttaa
     9001 agaaattcta ttagatatgt cttcggagga tgaaagatat tcataatttt catagtgcgc
     9061 tatttcatgg aattttgtaa atctattggt gacactcact ttgctagc
//
LOCUS       DAPHJE010000151         8846 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPHJE010000151 DAPHJE010000000
VERSION     DAPHJE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.153"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(166..1266)
                     /gene="chaA"
                     /locus_tag="RH222_003932"
     CDS             complement(166..1266)
                     /gene="chaA"
                     /locus_tag="RH222_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV3798876.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1536..1766
                     /gene="chaB"
                     /locus_tag="RH222_003933"
     CDS             1536..1766
                     /gene="chaB"
                     /locus_tag="RH222_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV3798877.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1924..2619
                     /gene="chaC"
                     /locus_tag="RH222_003934"
     CDS             1924..2619
                     /gene="chaC"
                     /locus_tag="RH222_003934"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV3798878.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="RH222_003935"
     CDS             complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="RH222_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV3798879.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3201..4595
                     /gene="ychO"
                     /locus_tag="RH222_003936"
     CDS             3201..4595
                     /gene="ychO"
                     /locus_tag="RH222_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV3798880.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASIDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4596..5246)
                     /gene="narL"
                     /locus_tag="RH222_003937"
     CDS             complement(4596..5246)
                     /gene="narL"
                     /locus_tag="RH222_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV3798881.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5239..7035)
                     /gene="narX"
                     /locus_tag="RH222_003938"
     CDS             complement(5239..7035)
                     /gene="narX"
                     /locus_tag="RH222_003938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV3798882.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7374..8765
                     /gene="narK"
                     /locus_tag="RH222_003939"
     CDS             7374..8765
                     /gene="narK"
                     /locus_tag="RH222_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV3798883.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2160 a         2150 c         2290 g         2246 t
ORIGIN      
        1 aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag
       61 gctcgcaatt ctgattaacg acctgtccag gtagtatgaa gaaatacaat aagcaggaat
      121 ttatattatc tcccccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt
      181 catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc
      241 ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc
      301 accaagtgca aactgtaatt cgttacccgt cataaaggca attagcgtga cgacaggtac
      361 ggtaagcgaa atggttgcta acactgaacc aaagaacaga ttcatcgcgc gctggacctg
      421 gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca acagagccac
      481 caggaagcca gtaaaggcga caggggcatt catgctgtcg agcaatgtct ccagcgggct
      541 ggcattcatt ttggtcaccg caataacggc aatcagatgg ataatcaacc agatagcatg
      601 ccacacgctg ctatgggcag acggtttacc gtgatgcggg tcatcgtcat cactgtcatc
      661 ttcgtgctcg tagacaaaca aactttgatg cgttttggtc tggatcagca aaaatacgcc
      721 atacattgcc gcagaaatta atgctaccag taacgcctga ccggttgaaa aattcgccgc
      781 aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata
      841 ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa
      901 tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat
      961 tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat
     1021 aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac
     1081 gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt
     1141 gatggcaatg accactggta gtgtctggct gcttccccac aggaacagca ctaccagcgc
     1201 cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt
     1261 tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa
     1321 aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc cactgtcgat
     1381 tttttactca ttgggacata aaaataagta ttacgtttag acaatgtttg ttttagcgtt
     1441 tcttaagaac agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc
     1501 acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc
     1561 tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt
     1621 tcaacagcgc atgggatcaa tataaagata aagacgatcg gcgtgatgac gccagtcgcg
     1681 aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg
     1741 atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg
     1801 ttgccttgca agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag
     1861 taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt
     1921 aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt
     1981 gaagaatcac tcttatggtc agcagagcaa cgggcggctt cgctggcggc gacgctggct
     2041 tgtcgacctg atgagggacc ggtgtggatc ttcggttatg gatcgttgat gtggaatccg
     2101 gcactggagt ttaccgagtc gtgcaccggt acactggttg gatggcatcg cgcgttttgc
     2161 ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa
     2221 gagggcggac gcactacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa
     2281 ctaaccctgt tgtggaagcg cgagatgatt accggctgtt atctgccaac ctggtgtcag
     2341 cttgatcttg atgatggatg cacagtaaac gccattgtgt ttattatgga cccgcgacat
     2401 ccagaatatg aatctgacac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc
     2461 ggtccgctgg gaaccaacgc acaatacctg ttttcactgg aacaggagct tatcaaactg
     2521 ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact gctggcggag
     2581 aattatccgg atggtgtgtt acggccggga ttcgcctgag taaacttccc tcatagtggg
     2641 gcgtcagacg ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt
     2701 gcgccagttc cacaagagta ccgatttcca ccccatcaat aagaggaagt gtgctaatcc
     2761 cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct
     2821 ccagcatttg ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga
     2881 ccgcatcaga catcaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa
     2941 tggccagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga
     3001 tcacgatttt ttgcattatt tactcctgta ttcagggaat tagacattca tcttctatct
     3061 tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg
     3121 agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat
     3181 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac
     3241 tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
     3301 atccctttga taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg
     3361 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
     3421 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
     3481 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagta
     3541 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
     3601 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg
     3661 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
     3721 gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg
     3781 ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagccg tttgctgcat
     3841 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtatgac ctgacagccc
     3901 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg
     3961 gtgagcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
     4021 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg
     4081 aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
     4141 tcaaaaaaca actttctgcg ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
     4201 atgataatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
     4261 cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
     4321 tacaaatccg cagtcgttat ggtattcggc aactgatttg gcagggcgat acgcagatat
     4381 taagtttgac gccaggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
     4441 actggcagaa cggggaaggg gcgagcaatc actggcgatt gtcggtggtg gtggaagata
     4501 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgacg
     4561 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
     4621 cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc
     4681 acttttactg tgctttcggt gatgtccagg cggcgggcaa tcatcttgtt cggcaaaccc
     4741 tgggcaatca gcttgagaat atcgcgctcg cgtggggtta actggttaac atcgcgttca
     4801 gtagtggcac ggttagcgcg caagctggct gccagaacag gcgttaatgc ctcgcttaat
     4861 accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct
     4921 tttaatagat agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta
     4981 gagacgctga ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt
     5041 tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagat
     5101 tccgccagtt caataccctg ttcgccatta ctcgcttcgc caactacggt gatatctggt
     5161 gccatactga taagctgttt cacgccagtt cgcagcatcg gatgatcgtc gatcagcagg
     5221 atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc
     5281 gggaataaag gtgacaacca cttcggtgcc acctgattca cgacggcgaa cgcggcaatc
     5341 gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat
     5401 ggcattttca ggcacgccgc agccgttatc ctggacggtc agtttgacct gattatcgtt
     5461 ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc
     5521 ctcacgggca atttgcaaca agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa
     5581 ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc
     5641 cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg
     5701 caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct
     5761 ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca tgcaagagag
     5821 agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat
     5881 caactgttgc tgacgttcct gatgccgatc cagcgccagc gtggcggtga gttgttcaac
     5941 cagggtatcc accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc
     6001 cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg tcgtgccgcg
     6061 atcaccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat
     6121 atctggctgg caggtaaact cctgatgatt ctcttcatca tcagtgtcat acacccgcag
     6181 ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtacaggtg acaggcgttc
     6241 acacagcggg gcgcgggaat gcaaacggcg gttagcctgc cataaaaaag agaggatctg
     6301 atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact
     6361 ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg
     6421 cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag
     6481 cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa
     6541 aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcaa tgcgcatttc
     6601 cgtggtgcgg tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc
     6661 cgctgacacc gtttctcggt tttgtgcacg catcagcgca gggatcagtt cattacgcca
     6721 gtaatcttgt aaaccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa
     6781 ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa
     6841 tggcactgcc gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc
     6901 atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc
     6961 aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag
     7021 acaacgttta agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga
     7081 atgtgacgat acaatctgga atggcagtat tctcggctat tggctgaagt atacccatac
     7141 ccggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg
     7201 gattctacag caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg
     7261 taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc
     7321 tttagctaca gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc
     7381 actcatccgc ccccgaaagg gctactggag ctgtcattac agattggcga ccggaagatc
     7441 ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg caacctgtgg atttccgttc
     7501 cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac
     7561 cgaaagtcgg ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcgg
     7621 tttctggcgc gttattacgt gttccatact cctttatggt tcctatcttc ggtggtcgtc
     7681 gctggacggc gttcagcacc ggtattctga ttattccttg tgtctggctg ggttttgccg
     7741 tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgctg tgcggctttg
     7801 ctggcgcgaa cttcgcttcc agtatggcaa acatcagctt cttctttccg aaacagaagc
     7861 agggtggcgc gctgggtctg aatggtggtc tgggcaacat gggcgtcagc gtcatgcagt
     7921 tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca
     7981 aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc
     8041 ttgccatctt caccattgcg gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct
     8101 ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt
     8161 atctggcaac cttcggctcc tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa
     8221 cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc
     8281 tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg
     8341 tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta ccgactgacg
     8401 ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg
     8461 ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg
     8521 atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca
     8581 cggcggcggc gctgggtttc atctctgcga ttggcgcgat tggtggcttc tttatcccga
     8641 aagcgtttgg tagctcgctg gcattaacgg gttcgccagt cggcgcaatg aaggtatttt
     8701 tgattttcta tatcgcctgc gtagtgatta cctgggcggt atatggtcgg cattctaaaa
     8761 aataaatctt aatatttgat tatcctttgc gcggcatgat gtcgcgcttt ttttatgcgt
     8821 catttagtta caacatacta atgtta
//
LOCUS       DAPHJE010000152         8806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPHJE010000152 DAPHJE010000000
VERSION     DAPHJE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.154"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1167
                     /locus_tag="RH222_003940"
     CDS             <1..1167
                     /locus_tag="RH222_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV3798884.1"
                     /translation="FAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIM
                     IAFLVARNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAV
                     IVALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLI
                     SPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSE
                     ANLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRD
                     LAKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAY
                     AVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1195..1506
                     /locus_tag="RH222_003941"
     CDS             1195..1506
                     /locus_tag="RH222_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV3798885.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1562..3235
                     /locus_tag="RH222_003942"
     CDS             1562..3235
                     /locus_tag="RH222_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV3798886.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3260..4699
                     /locus_tag="RH222_003943"
     CDS             3260..4699
                     /locus_tag="RH222_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV3798887.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4756..4974)
                     /gene="marB"
                     /locus_tag="RH222_003944"
     CDS             complement(4756..4974)
                     /gene="marB"
                     /locus_tag="RH222_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV3798888.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5006..5389)
                     /gene="marA"
                     /locus_tag="RH222_003945"
     CDS             complement(5006..5389)
                     /gene="marA"
                     /locus_tag="RH222_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV3798889.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5409..5843)
                     /gene="marR"
                     /locus_tag="RH222_003946"
     CDS             complement(5409..5843)
                     /gene="marR"
                     /locus_tag="RH222_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV3798890.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6055..6720
                     /gene="marC"
                     /locus_tag="RH222_003947"
     CDS             6055..6720
                     /gene="marC"
                     /locus_tag="RH222_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV3798891.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6745..7935)
                     /gene="ydeA"
                     /locus_tag="RH222_003948"
     CDS             complement(6745..7935)
                     /gene="ydeA"
                     /locus_tag="RH222_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV3798892.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8085..8806)
                     /gene="yneK"
                     /locus_tag="RH222_003949"
                     /pseudo
     CDS             complement(8085..8806)
                     /gene="yneK"
                     /locus_tag="RH222_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2406 a         1892 c         2115 g         2393 t
ORIGIN      
        1 tttgccatat tatcgccaga cggttttatt gccggattac tgcatctgaa tagcattttc
       61 cccaaccttg aaaaagcaca agctattttt actccggtaa tgaatggttc tgtaaatatc
      121 atgtcaatta tgattgcttt cctggtcgcc aggaatatgg cgattagcta tgagcaagat
      181 gatcttttat gcggattaac ggcaatagga gcatttttta ttgtttatac gccatatcag
      241 atgatagatg ggcaagcatt cctgacgacc aaatatctcg gcgcgcaggg gttgtttgtt
      301 gctgttatcg ttgcattgat caccagtgaa atattttgtc gcttagctcg aaacccaaaa
      361 atcaccataa cgatgccggc agctgtaccg cctgcggtag cgcgttcatt taaagtttta
      421 ttgccaatat tttttgtcat ggtgttcttt tccgcactta attattgcct gacactgata
      481 tccccggcag gactaaacga tcttatttac acattaatcc agacgccgct caaacatatg
      541 ggaacgaata tctttgcggt aattatcctg ggggctgtgg gtaatttcct gtgggtgctg
      601 gggatccacg gacctaatac cacatcggca attcgagaaa ccgttttttc tgaggctaat
      661 ctggagaatc tctcctgggc cgctcaacat ggcactacct ggggcgcgcc atatccgatt
      721 acctggactt ctattaatga tgcattcgcc aactgcggcg gttcaggtat gacgttgggg
      781 ttattgttgg ctatttttat cgcttctaag cgtgcggaat accgtgatct ggcaaaaatg
      841 tcatttatcc ccggtatttt caatatcaat gaaccgataa tgtttggcct tcctattgta
      901 cttaacccca tcatgatggt gccgtttatt atggttccca ttgttaactg tgccattggt
      961 tacttctttg tttcgatgga aattattcca ccggttgctt atgccgtgcc ctggactacg
     1021 cccggacctt taattgcttt cctcggaacc ggggggaact ggctggcttt actggttggt
     1081 tttttatgtt taggtgtggc gacaatgatc tatttacctt ttgttattgc cgccaacaaa
     1141 gtcaataaca tgacaactaa cggataattc catttaccca ggaaggagat tgtgatgttt
     1201 gcagatgaag aacttgttat ggagttactg gtcaacgcgg ggcaggcacg ttctgacgcg
     1261 atggaagcca tacgttgtgc cgggcaaaaa gactggcagg gcgcaactca gctaatggcc
     1321 agttctgaat ctgcctgtct tcaggcacat aagattcaga ccgcattaat tagtcaggat
     1381 gaaggctgtg gcaaaataaa agttaattta attctgattc atgcacagga tcatttgatg
     1441 aatgcaatat tatgccagga tttagccagg gaaattattt cattaagaaa agaactccat
     1501 gcctgatttt ttcctccagt gcgatagctg gaggaaatta ttatactctt aaggataagt
     1561 catgaatata aagacgctta acgtcagcct tttgtctttt tctattatta cagcattgtt
     1621 tccattgaac gcgatggcaa caaaattaac catagagcag cgccttgaat tgcttgaaaa
     1681 tgaattgtcg caaaataaac aagagctgaa agcaacacag aatgaactgg gagtatataa
     1741 atcccgactt tcgaccttac aaaaaagcat cacggaaaac aaatataaat cagcctcgct
     1801 tgctgaaata tcagcgacat ctcccgtcgc tgataacatc aaaaatgaaa acggtgaaca
     1861 gaactcgttt accgcagcgc atactataaa tggatcgcag caagttgccg ttattgaaag
     1921 taaaggcgac aaaaccacta tcgaaagcgt gaccctgaaa gatatcagta aatatataaa
     1981 ggatgatatt gggttcagct atcaggggta cttccgctca ggctggggta ctggtaatca
     2041 cggctcacca caaacttatg cagcgggttc tctgggacgt tttggtaatg agatgagcgg
     2101 ttggtttgac ctgaccttaa atcagcgtgt ttataatcag gacggtaaaa cggcaaatgc
     2161 ggtcgttacc tatgacggca acgtgggtga gcagtataac gatgcctggt ttggtgacag
     2221 tgccaatgaa aatatcatgc agttcagcga tatttacctg acaacgcgag gttttttacc
     2281 cttcgcacca gaggcagact tctgggtcgg caaacataaa ctcccgcaat atgagatcca
     2341 gatgctggac tggaaaacct taaccacgga tgtcgccgcg ggtgtgggga ttgaaaactg
     2401 ggcacttggt gtagggctgt ttgatatgtc cttaagccgt gatgatgtcg atgtttactc
     2461 tcgtgatttt tcgcgtacca gccagatgaa taccaattct gtggatgttc gttatcgcaa
     2521 tatcccgtta tgggatgatg cgacattatc attaatggct aaatattccg cacctaataa
     2581 aacggatcaa caacaagata acgaaaatga caacagttat tttgaaatga aagatagctg
     2641 gatgctgact tctgttttac ggcaaaacct gcaacgcgat acgtttaatg aatttacgtt
     2701 acaggttgcc aataattcct atgccagcag ttttgccagt ttctcagatg ccagtaatac
     2761 gatggcgcat ggtcgctatt actatggtga tcataccaat gggatcgcct ggcgtttaat
     2821 ctctcagggc gagatgtatc ttactgacaa tattattatg gctaacgcgc ttgtctattc
     2881 tcacggcgaa gatgtttata gttatgaaag tggtgctcat agtgattttg acagtattcg
     2941 cactgtaata agaccggcct ggatctggaa tacatggaat cagacggggc ttgaattagg
     3001 ctggtttaag caacagaaca aaactcagca gggtgtaaca ctaaatgaat cggcttataa
     3061 aacgacactc tggcatgcat tgaaagtggg tgaaagcatt ttaggttcac gaccagaaat
     3121 tcgcttctat ggaacgtata tcaatattct ggataacgaa ttatcgaatt ttaagtttaa
     3181 tgagaacagc aaagacgagt ttatggccgg catccaggcg gaagtctggt ggtaatctat
     3241 tttagttaag gagttgtgta tgtcaggatt taaaaaaggt tttttatggg gcggtgcggt
     3301 agccgcgcat cagttggaag gtggctggaa tgaaggagga aaaggcatca gcatcgctga
     3361 tgtgatgact gctggcgctc acggggtgcc gcgtgaagtg acagaaggcg ttatcgacgg
     3421 gcttaattat cccaatcatg aagcaattga tttttatcat cgctataaaa cagatattca
     3481 gttatttgcc gagatgggat tcaaatgctt tcgaacttcc attgcctgga cacgaatctt
     3541 tccgcaaggt gacgaacagg agccgaatga agagggttta caattttatg atgatctgtt
     3601 cgatgaatgc cttaagcagg gaatggaacc tgtggtgacg ctttcacatt ttgagatgcc
     3661 ttatcatctg gtgacaaaat atggtggctg gcgaaaccgt aaactgatcg acttttttat
     3721 ccgcttcgca tcaacggtct tcacgcgcta taaagaaaaa gtaaagtact ggatgacgtt
     3781 taacgaaatc aataatcagg tgaatttcag cgaaagcctg tgtccattta ctaattccgg
     3841 tatcttgtat tcgccagagg aagatatcaa tgagcgcgaa caaataatgt accaggcggt
     3901 acattacgag ttagttgcca gtgccctggc ggtacagact ggaaaatcga tcaatcctga
     3961 atttaacatt ggctgtatga tcgccatgtg tcccatctat cctctgacgt gtgcacccaa
     4021 cgatatgatg atggccacga aagcaatgca tcgtcgttac tggtttactg atgttcatgc
     4081 tcgtggatat tatccgcaac atatgctgaa ttactttgcc aggaaaggat tcaacctcga
     4141 tatcacacca gaagataacg cgattcttgc cagtggttgt gtcgacttta tcggctttag
     4201 ctactacatg tcttttacga cacaattttc gcccgataac ccgcaactgg attatgttga
     4261 accacgagat ttggtcagca acccttatat cgatacatcc gaatggggat ggcaaattga
     4321 tccggcaggg ctacgttatt cactcaactg gttctgggat catttccagt tgccgctgtt
     4381 tattgtcgaa aatggatttg gtgcggttga ccagagacaa gctgacggca cggtgaacga
     4441 tcactatcgc attgattact ttgcttccca tattcgggaa atgaaaaaag ccgtagttga
     4501 agatggtgtt gacttaattg gctacacccc gtggggctgc attgacctgg tttctgccgg
     4561 aacaggggaa atgaaaaagc gctacggaat gatttatgtc gataaagaca acgaagggaa
     4621 gggaacgctg gaacggatac gtaaagcgtc gttttactgg tatcgggatc tcatcgccaa
     4681 caatggcgaa aatatttgag acttgaggaa gatattacgg agatgtcagg ctctgtgatc
     4741 agagcctgac aataactaca tagcgtgttg attataatag ggcacgccga gcgcatccga
     4801 cttatcactg ccagtaccca tgtgatttaa atcaaacggt ggttgttcct gcgatggggg
     4861 aacaaccacg acattggcac aagaagtaac aactggctgc gtggtttgtt ccgcaacgcc
     4921 ctgcgcggaa aagagaataa gcgcagctgc tattgcggat gaaagtggtt tcatgattgc
     4981 ctcagtgacg ttgtcacgtt atcaactagc tgttgtaatg atttaatgga tgtaaaaagc
     5041 gcgattcgcc ctgcatattg gtcatccggt atttatgcgg cggaacatca aagtaatttt
     5101 tgaaggttcg ggtcagagtt tgttgcgact cgaagccata tcgttctgcc agatagagta
     5161 tcggctcgtt actttccttc agcttttgcg cgatttccgt catcttacgg ctgcggatgt
     5221 attggcctaa tgaatgaccg gtttcttttt taaacatccg ttgcaggtgc catttagagt
     5281 aacctgaacg ctctgacact ttctccagtg acagtggcga ttccaggttg tcctcaatcc
     5341 agtccaaaat gctatgaatg gtaatagcgt cagtattgcg tctggacatc gtcatacctc
     5401 ttttttgttt acggcaggac tttcttaagc aaatgctcaa gtgttgccac ttcgtccgcc
     5461 gtcaggtttt ttgttaattc ttgatgcagg tcctggccaa ctaattgatg gcattgttcg
     5521 catattgccg cgccgctggt ggtaagtttt accagtacgc cgcgcttatc attcgggttc
     5581 ggcaaccttt caacccagcc tttacagacc aggcgatcca gcatacgggt cagtgctcca
     5641 aggtcgacag acaacacttt tttcagttca accggagtaa tacacgccgc gcagcggata
     5701 gagcagagca ccttaaactg tgccgcggta atatccagcg gagacagata ctcgttaagc
     5761 agacgatctt tcttctgatt aaccatatgg attaagcgac ccaatggaat aatttcattg
     5821 aacagatcgc tggtactttt cacattagtt gccctggcaa gtaattagtt gcaggggata
     5881 atattgccca ggcaagtata agtcaaataa atgaattgac cgatgccacg ttttgctaaa
     5941 tcggttcaag ttttggtcaa tgcgatgcaa ggttagctaa agagtgacac tttatccttg
     6001 tttacccctt atacttttcg ctgataaccc agatacacag gataacaacc accaatgtta
     6061 gatttgttta aagcaattgg cctggggctg gtggtgttac tgccgttagc taacccatta
     6121 acaactgtcg cgttatttct tggcctggca ggcaacatga acagtgccga acgtaatcgt
     6181 cagtcgttga tggcctcggt gtacgtattt gccatcatga tggtggcgta ttacgccggg
     6241 caactggtga tggatacatt tggcatttcg attcccggtc tgcgaattgc aggcggctta
     6301 atcgtcgcgt ttatcggttt tcggatgctc tttccgcaac agaaagcgat tgattcaccg
     6361 gaggcgaaaa gcaagtcgga agagctggaa gatgaaccca gtgccaatat tgcctttgtg
     6421 ccgctagcaa tgccaagtac cgctggtccg ggaaccatag cgatgattat tagctcggcg
     6481 tccacagtgc gtcagagttc aaccttcgct gattgggtac tgatggttgc gccgccgctg
     6541 atctttttcc tggtggcggt tattttgtgg ggaagcctac gcagctccgg cgcaattatg
     6601 cggctggtgg gcaagggtgg cattgaagcg atctcccgtc tgatgggatt cctgctggta
     6661 tgtatgggtg tgcagtttat tattaacggc atcctggaaa tcattaaaac gtaccattaa
     6721 aaaaggcccg aatgggcctt tcaactattg cgtctgttct tcgagtgtca ctggccagcg
     6781 gcgaaatata atgattgacc aaattaacgc ggcaaaagca ggcaccgcgc ccacataacc
     6841 aatcatcgac attgaccagt gcaaactcac ctgattacct accaacgcac ccgccccgat
     6901 tccaatatta aatatgccgg agaatagcgc catcgcgacg tcggtagcat ctggtgccag
     6961 cgccagcact ttaacctgca taccaagccc gatgatcatc atcgcgatcc cccagaaaat
     7021 actcagcacc ccgaggtgta tttcactgtt cgccgcaggt aacagcaatg ccaggcacac
     7081 cagcaacagc gcaatcgccg tactcaccaa cgcagacgca tactgattac ccagtttacc
     7141 gaaaatcacg ctgccaataa tgcccgaacc accgagtaat aacagtaatg ccgtggcaaa
     7201 gttggcgctg aatcccgcaa tgttttgtac aaaaggctcg atatagctgt atgccgtgta
     7261 atgggcggtg acaaccacca cagttaacaa ataaatgctc atcaatgccg ggcggcggaa
     7321 caatagcggg aggcttttca gtgaaccgga atgctcactg ggcagtaagg gaagtaactt
     7381 aatcaggcac aaaagggtga taagcgcccc aataccaatc gcgaagaagg tcattcgcca
     7441 accgaaatac tggcccacaa tgcgcccgag aggtaaacct aagaccatcg ccagtgctgt
     7501 accggtggca attaaactca atgcctgtgc tcgcttcccg gccggagcca tacggatcgc
     7561 cagagatgcc gtaatcgacc agaaaatcgc atgtgcaaaa gccacaccaa tgcgactgat
     7621 caccagaacg gtaaagctcc acgacaaaaa cgacagtacg tggctggcaa taaacaccac
     7681 aaacaggcag atcagtaatt tgcgccgttc aacctgactg gtcattaaca taaaaggcaa
     7741 tgacattagc gctactaccc atgcgtaaat ggtcaacatg atgccgacct gagcggtttg
     7801 catgtgaaaa ctttgcgcaa tgtcagagag caggccaaca gggacaaatt cggtggtgtt
     7861 gaagatgaag gcggcgactg ccagcgtaac gacccgtagc cacgccactt tgcgggaaac
     7921 agtgtttgtt gtcataacag attatcgggt gagttgcgaa aaagtgatga aatgatctta
     7981 aaacggttaa tgatgtgaat acaatgcatt tgtgatctgg atcgcgtttt ccgtttctct
     8041 gacgctgatt ctgttccgcg tcagagagtc ggtaacatca cggtttaatg acgttcaggg
     8101 aggtctggcg ttttggcggt tgcgggtata ggtggtctga caggggagcg aggcctgttt
     8161 ttcaccaggt cttcgtgagt aacttttggc ggcgtttcat tttttacaaa cagtttgagg
     8221 gtcctgcgcg ccatatgttt gataatttta tcatcactta atgatgctgg catcaacacg
     8281 tcattcagat cactttcatt gggattcata aattggtgat agtgcaataa catattggcg
     8341 aaaaataaaa aaaaagtcct gaaaatgagg agattcttgt tctgctaaaa gggataaaaa
     8401 gcagatgcag acttcgggtt gtacatgttt gttagaacct aatgaatcca tcaggctttg
     8461 caataaatcc tcttttaatt cgccgtctgt tatgtcgaga atggcggtga tcaagttagg
     8521 atctttattg acgaaccatc tgtgaacctc agagttactc cataatcggg ggagtatgac
     8581 aaaattaccg cgagagataa aattcaggac aagcaaatca aactggcgta agtaatctgc
     8641 agcggtatgg cgatccggag gcaggggttc aatatgtggt gtatgcgctc ttttgatggg
     8701 aacgccaagg ttgatcattg gaaattgttc aataaaatca ttcagaaaca gaaatagcac
     8761 acgctcgacg ccggtctgaa atacgcgccc catagtgaca ggcttt
//
LOCUS       DAPHJE010000153         8728 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPHJE010000153 DAPHJE010000000
VERSION     DAPHJE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.155"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            5..217
                     /locus_tag="RH222_003950"
     CDS             5..217
                     /locus_tag="RH222_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV3798893.1"
                     /translation="MTRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIP
                     GVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            291..659
                     /gene="cbtA"
                     /locus_tag="RH222_003951"
     CDS             291..659
                     /gene="cbtA"
                     /locus_tag="RH222_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDV3798894.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            748..1121
                     /gene="cbtA"
                     /locus_tag="RH222_003952"
                     /pseudo
     CDS             748..1121
                     /gene="cbtA"
                     /locus_tag="RH222_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            1118..1312
                     /locus_tag="RH222_003953"
     CDS             1118..1312
                     /locus_tag="RH222_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798895.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1325..1438
                     /locus_tag="RH222_003954"
     CDS             1325..1438
                     /locus_tag="RH222_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV3798896.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(1727..1873)
                     /locus_tag="RH222_003955"
                     /pseudo
     CDS             complement(1727..1873)
                     /locus_tag="RH222_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1927..2109
                     /locus_tag="RH222_003956"
     CDS             1927..2109
                     /locus_tag="RH222_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDV3798897.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2210..2539)
                     /locus_tag="RH222_003957"
     CDS             complement(2210..2539)
                     /locus_tag="RH222_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV3798898.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2711..3769)
                     /locus_tag="RH222_003958"
     CDS             complement(2711..3769)
                     /locus_tag="RH222_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3798899.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3967..4440)
                     /gene="sbmC"
                     /locus_tag="RH222_003959"
     CDS             complement(3967..4440)
                     /gene="sbmC"
                     /locus_tag="RH222_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV3798900.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4559..5725)
                     /gene="dacD"
                     /locus_tag="RH222_003960"
     CDS             complement(4559..5725)
                     /gene="dacD"
                     /locus_tag="RH222_003960"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV3798901.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5934..7361
                     /gene="sbcB"
                     /locus_tag="RH222_003961"
     CDS             5934..7361
                     /gene="sbcB"
                     /locus_tag="RH222_003961"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV3798902.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(7404..7631)
                     /locus_tag="RH222_003962"
     CDS             complement(7404..7631)
                     /locus_tag="RH222_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV3798903.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7645..8703)
                     /gene="yeeE"
                     /locus_tag="RH222_003963"
     CDS             complement(7645..8703)
                     /gene="yeeE"
                     /locus_tag="RH222_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV3798904.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVVSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         2310 a         2261 c         2036 g         2121 t
ORIGIN      
        1 aattatcacc cgtggtgaag ccatgcgtat tcaccaacaa catccgacat cccgtctttt
       61 tccgttctgt accggtaaat accgctggca cggcagtgct gaagcgtata ccggtcgtga
      121 agtgcaggat attcccggtg tgctggccgt gtttgctgaa cgccgtaagg acagttttgg
      181 tccgtatgtc cggctgatga gcgtcaccct gaactgagtg ggaattctga tgagcagaat
      241 tatcgccact accggaccat tcttagccga ttttctgtaa ggattttatc gtgtcagaca
      301 cactccccgg gacaacactt cccgacgaca atcacgaccg cccctggtgg gggctgccct
      361 gcaccgtgac gccctgtttc ggggcacgtc tggtgcagga gggtaaccgg ttgcattacc
      421 ttgccgaccg cgccggtatc agaggccggt tcagcgatgc ggatgcatac catctggacc
      481 aggcctttcc actgctgatg aaacaactgg aactcatgct caccagcggt gaactgaatc
      541 cccgccatca gcataccatc acgctgtatg caaaagggct gacctgcaaa gccgataccc
      601 tcagcagttg tggttacgtt tatctggctg tttatccgac gcccgaaatg aaaaattaac
      661 tctccagaat agccttctgc tacggcctgg tgttttcacc acgccacttt tccattttta
      721 tatctgcata tcaggaaaat cttcagtatg aaaacattac ctgtattacc cgggcaggcg
      781 gccagttctc gcccgtctcc tgttgaaatc tggcagatac tgctgtcccg actgctggac
      841 cagcactatg gcctcacact gaatgacaca ccttttgccg atgaacgtgt gattgagcag
      901 catattgagg caggcatttc actgtgtgat gcggtgaact ttctcgtgga aaaatacgcg
      961 ctggtgctac cgaccagccg ggattcagcg cctgtacccg ctctcagtta ataaacagca
     1021 tcgatatcct ccgggctcgc agggcgaccg gcctgatgac ccgcgacaat tacagaacgg
     1081 taaataacat taccctgggt aagtatccgg aggcgaaatg atgacactgg aagccgacag
     1141 cgttaacgta caggcgctgg atatggggca cattgtcgtt gacattgatg gtgttaatat
     1201 cactgaactg attaataagg ccgctgaaaa cggttattca ctccgcgtgg tggatgaccg
     1261 tgactctacc gaaacaccgg caacttatgc cagccctcac cagttgctgt aagacaatgc
     1321 agtgatgcag ggtatagtgc cattaaccac agaaacagca ctgttgtcct gtattaagca
     1381 ggcaagagaa gatgtcctta ccctgcgcca tctgcagctt ctgcaccaga accggtgaat
     1441 ctcactcaac aggcttcatt tcccctgacg ccagcctgaa tacagctggc gttttcattt
     1501 ataaacagaa aggaaaaccg ataataatgg aatggacgcc ccgaccatga agcaacggta
     1561 ggatattact gataaacctt ccagtggagg tgcgttatgg ccgttttaac gattagcatt
     1621 gacctgacaa aaaatgaatt ccaaatccac ggtctgggta ggaacagaaa aatctaagct
     1681 cagaaaacgg attaaagtaa gcgtcaacgg agcaccgtat tgacgcttat ttattggtga
     1741 gaactacgtt ccatggcagg agttcgtcaa cacggttgga gggccattcc ggcagtatgc
     1801 tcagaatatg gcgcagatac gcttccggat cgataccgtt cagacggcag gtgcctgctt
     1861 ttccggcaac aagcaggaat ctcagcgctg aagcaggaat tagaggtaaa aacgccctac
     1921 cgggccatga atcacccggt cattggagta gtaaccaaag cagatttagc cagcatggaa
     1981 caaatatctt tggtgaaaag ttggttacgg gaagctggag cgcacaacgt gttagtaacc
     2041 agtgcagtta ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagaaggc
     2101 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
     2161 caatgagcaa tgagggttac cgggcaaccc tcattgaata aaacgggaat tacttcgctt
     2221 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
     2281 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
     2341 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
     2401 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
     2461 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttctagtacg tcctggaatg
     2521 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
     2581 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
     2641 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
     2701 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
     2761 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccaca accttcaggt
     2821 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
     2881 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
     2941 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg
     3001 ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa
     3061 tggtctggat accttcatat atcgatttgg gaatacgggt ttctttgctg gcgggcgcaa
     3121 tcagcccacg cattttcacg gcatcggtca gcagtttttg tagatggctt tccagacgtg
     3181 ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac
     3241 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     3301 acattgccag taaagagccg aggatcacat cgccgcttcg ccataacgcc gtatcaattt
     3361 cacctgtcgg ggaacccaca acaattgcca gcgtcacccc aatcaataat ccttgatacg
     3421 gtttcttgcc cagcgccagc caaccgcaaa gaaacatcgc cgccgcgcac cagactaaca
     3481 tcagcggtaa cgaaattaac tccagttgca gagcgataag acctaaaatc gaccccaaca
     3541 ccgtaccgcc aatacgttcg aaggcacgag ggacaacgtt cccccagaac gagattggcc
     3601 ccataatcac caccatggtg accagcggcc aggtgctttc cgggatagta aacaggcgga
     3661 taatgagaaa agtgagcagg aatgccagcg cgacccgagt accatgcaca atgcggtaat
     3721 ggcggtatac ccggatttca aacgggctta atgacttatc ggcacgcact cagaaactcc
     3781 gtaatgaaga tgaaaagcgc tcataacaca tctgtcggcc agaaggcgcg cctctgagtt
     3841 atccgacgga cataccggga acagtgtaat ttcccggcag gagcgaaaaa tttgaaaggc
     3901 gcatcttact cttttcgctt tcaaaaaaag agtggtcttc gcgttaacac accgccctga
     3961 gatgaattag tgatgttttg gctgcaccgc aacatacatt tcgatatccc agtacccatc
     4021 ttccgcgcca ttgttcaagt aaacctcgaa gcagggcttt ggtaacattt cataagcact
     4081 gtcctgcaag aggctattaa agaactgata ccagggttta gcaaaatcat caccgactac
     4141 acgagccacc gccaccgcat attgaccacc tgaaatttct gtcagaatga cgccctcact
     4201 gttttcgggg agcgtaaagt tattcggtac cgtcacgacg gtgtcgcagc gtaatttttc
     4261 ggcgggtgtt tcatccggat tgtcgtaata gacagcaacc cactccttcg gcacaatatt
     4321 tttgctatct acccacatca tcaactgctc aaaacctttc tttaccgtct gttcccacgg
     4381 gccaacgaga tggaaacctg caacggtacg tttctcttcc tgcttaatct cgtagttcat
     4441 gacgcctcca ttgatgctgt ttttatatac agtatagttg caaaattaaa accacaagga
     4501 atgagtgttg attatgcgag cagactcgca ctcctgccag tctgctgcaa aagaaaggtc
     4561 aggccttatg gtggaaataa tcactcaggc gagaaaacat gctgccttcc ccgacagatt
     4621 ccagggtaac cagcggccag tgcgccacct gtttatcacg gtcgtaaagt tcaatttccc
     4681 ctacccgctg atgggcgcta attggcgcgg tgagctcttt accatcaagg gtatatttgg
     4741 ctttgatatg tggaatttcg gctttcggta gcaccatcca gaactcttgt tccgttccca
     4801 ggtcgatatt ttctttatcg ccataccaga tgcgttccgt tccgaccttt ttcccacggt
     4861 gcaaaatttg caccgtagta aagttttgtt gcccccaacg cagtaatttt cttgcctctt
     4921 cctcacgacc ttttgcactg tcagccccca ttaccactgc aatgagacga cgctgcccat
     4981 ctacagccga agcaatgaga ttaaacccgg caccagaagt atgacccgtt ttcaggccgt
     5041 caacattcat ggttttatcc cacaacaacc cgttacggtt ttgctgggtg ataccgttcc
     5101 aggtgagact tttctcactg tacatatgat aaaactcggg ctcgccgtgg atgatagcgc
     5161 gagaaagcac agctaaatca taagccgagc tatgctggcc aggtgcatcc agaccatgca
     5221 ctgtttcaaa atgcgtatcc ttgagatgca gcttctcggc atagttgttc atcatttcaa
     5281 caaactgccg ttgcccaccg gcaatatagt cagccagagc aacacaagcg tcatttccgg
     5341 aatccacaat taaaccacgg cttaaatcac gtaccgatac gcgatcgccc tctttcaaaa
     5401 acatcagtga agaaccgaca aacaccggat tatctttcgc ccacgcatcg cgccccacgg
     5461 tgacaatatc gtctggcgta atgcgatgac tatcgatagc gcgatccacg acataacccg
     5521 tcatcagctt tgtcaggctg gcgggattgc gctgttgatg ctcattaccc gcggtgagga
     5581 tctgaccggt ggtgtaatcc atcagtaccc aggacccggc atgaatctgt ggaggctgag
     5641 gtgaaaaagg aatgttttcc gccgcaaaac cagacgataa gttaaaaatg aacaaagaag
     5701 cagcaataat aagacggcgt ttcaacagca aaccctcagg agtttcaaat agctgttctt
     5761 tttacggaaa tacttatgaa ctggctggaa taaagtgcaa gaaaatgtga ctaccctctc
     5821 atttttatct gacatgatct gttgtcactc gctgtcaaat tgtcgcgcta aagctgatta
     5881 gcacggtgat atttgatact ctggcagaca gcagaaataa cggatttaac ctaatgatga
     5941 atgacggtaa gcaacaatct acctttttgt ttcacgatta cgaaaccttt ggcacgcacc
     6001 ccgcgttaga tcgccctgca cagttcgcag ccattcgcac cgatgacgaa ttcaatgtca
     6061 tcggcgaacc cgaagtcttt tactgcaagc ccgcggatga ctatttaccc cagcctggag
     6121 cagtattaat taccggtatt accccgcagg aggcacgagc gaaaggagaa aacgaagccg
     6181 cgtttgccgc ccgtattcac tcgcttttta ccgtaccgaa gacctgtatt ctgggctaca
     6241 acaatgtgcg tttcgacgac gaagtcacac gcaacgtttt ttatcgtaat ttctacgatc
     6301 cttacgcctg gagctggcag catgataact cgcgctggga tttactggat gttatgcgtg
     6361 cctgttatgc cctgcgcccg gaaggaataa attggcctga aaatgatgac ggtctaccga
     6421 gctttcgcct tgagcattta accaaagcga atggtattga acatagcaac gcccacgatg
     6481 cgatggctga tgtgtacgcc actattgcga tggcaaaact ggtaaaaacg cgtcagccac
     6541 gcctgtttga ttatctcttt acccatcgta ataaacacaa actaatggcg ttgattgatg
     6601 ttcctcagat gaaacccctg gttcacgttt ccggaatgtt tggggcatgg cgcggcaata
     6661 caagctgggt ggcaccgctg gcgtggcatc ctgaaaatcg caatgccgta attatggtgg
     6721 atttggcagg agacatttcg ccattactgg agctggatag cgacacattg cgcgagcgtt
     6781 tatataccgc aaaagccgat cttggcgata acgccgccgt tccggttaag ctggtacata
     6841 tcaataaatg tccggtactg gcccaggcga atacgctacg cccggaagat gccgaccgac
     6901 tgggaattaa tcgtcagcat tgcctcgata acctaaaaat tctgcgtgaa aatccgcaag
     6961 tgcgtgaaaa agtggtagcg atattcgtgg aagccgaacc gtttacacct tcagataatg
     7021 tggatgcaca gctttataac ggctttttca gtgacgccga tcgtgcagca atgaaaattg
     7081 tgctggaaac cgagccgcgt aatttaccgg cactggatat cacttttgtt gataaacgaa
     7141 ttgaaaagct gttgttcaat tatcgggcgc gcaacttccc ggggacgctg gattatgccg
     7201 agcagcaacg ctggctggag caccgccgcc aggtcttcac gccagagttt ttacaaggct
     7261 atgcagaaga gattcagatg ctggcgcagc agtatgccga cgataaagaa aaagtggcgc
     7321 tgttaaaagc gctttggcag tacgcggaag agattgtcta atgtgaagcc tccgccgctg
     7381 gtacggcgga ggacaatttt gagtcaggct ttttgaacgg tgatgctcca cgctgcatcg
     7441 ccaatctgct gataatcggt gatggcatgt ccttcttctg ccgcccactg tgggattgct
     7501 tcagttgcct gggtgcaatc aaactcaatt accaactcat cgccgctggc catttctgcc
     7561 agtgctgctt ttgcttctat gagtggaaac ggacagacct gcgtcaccac atccagcttt
     7621 ttaatcgcca tctttacacc ttacttaatt tgccgcagca gttgccagtc gtgccttacg
     7681 ctgcggtcga acatacacaa gccaggacgc agtccagact ccgagaatca taaatacaag
     7741 gccaatccag ccctgccagg tcatcattgc agtcatgacc agtccattac cgatagagca
     7801 accacctgca atgctggcac cgaagcccat cagtacacca cctaacccgc tacgtaatgt
     7861 tgtttgtgca tcagcagcgc gaacgcggaa ctcacggctc gctttggctg caataaatga
     7921 ccccacgaag atccctaaca ccaggaaaac gccccagtta atgtatttca cgtcacccgc
     7981 gaccagaaat tgcagaatat tggccgttgg ggaagtgatt cctaacccaa acatgcgtcc
     8041 ggttgcttca ctcaggggcc aggctaaaag cgcaatcaaa ccgatgagta cagcagtgac
     8101 aaagggatgc cagcgttttt caaacagaat atgagcgatc ccggttcggc gcggcggtaa
     8161 ggtcgcgact ttgagttttg gcttcttcaa ctctttcatc accacccaga gcgttatcac
     8221 cagcaaaacg gcaaccaacg gccacacaga caaattaaaa gtatcagcaa tagagttatg
     8281 ttcagtagtg tagtgctgca aggtttgatt taaaccactg gcgtgtggag aacgcatcac
     8341 cgcactcacc accatataag tgaaaagcgc gatccaactg ccgatcaatc cctcacccgc
     8401 gcgataccag gtcccggtgg cacatccacc cgccagaaca attcctagcc cgaaaagata
     8461 cccacctata acagtaccaa gccacgggaa cgccccggct tcgtaagtca gtaggcccgc
     8521 ctgaattaac gcaaaaaccc ccacgctttg taccgaaatt gcaatcagca aggcgtaaaa
     8581 catgcgatta tttttcgcga tatacatatc gcgaaaacca cctgtcaggc aaaaacgccc
     8641 acgctgcatg acaaatccca gcagagcacc acaaattagc ccgcttaata tcattgaaaa
     8701 cataaattaa ttaaccagat gaatgtta
//
LOCUS       DAPHJE010000154         8498 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPHJE010000154 DAPHJE010000000
VERSION     DAPHJE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.156"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(197..1087)
                     /gene="malG"
                     /locus_tag="RH222_003964"
     CDS             complement(197..1087)
                     /gene="malG"
                     /locus_tag="RH222_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV3798905.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1102..2646)
                     /gene="malF"
                     /locus_tag="RH222_003965"
     CDS             complement(1102..2646)
                     /gene="malF"
                     /locus_tag="RH222_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV3798906.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2800..3990)
                     /gene="malE"
                     /locus_tag="RH222_003966"
     CDS             complement(2800..3990)
                     /gene="malE"
                     /locus_tag="RH222_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV3798907.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4355..5470
                     /gene="malK"
                     /locus_tag="RH222_003967"
     CDS             4355..5470
                     /gene="malK"
                     /locus_tag="RH222_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV3798908.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5542..6882
                     /gene="lamB"
                     /locus_tag="RH222_003968"
     CDS             5542..6882
                     /gene="lamB"
                     /locus_tag="RH222_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV3798909.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7125..8045
                     /gene="malM"
                     /locus_tag="RH222_003969"
     CDS             7125..8045
                     /gene="malM"
                     /locus_tag="RH222_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV3798910.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8274..8498
                     /locus_tag="RH222_003970"
                     /pseudo
     CDS             8274..8498
                     /locus_tag="RH222_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2113 a         2192 c         2189 g         2004 t
ORIGIN      
        1 acgaaagaga attatcgtaa gtgggaaaac aaataacgta aaaataataa gttctatgat
       61 ggaaatatta accggcgaac gattcagatt gcagacgaaa gaaaaaaagg cgctccgtgg
      121 agcgccgaat aacagtcaca agttgggata acgtaagttg agggtgcagc ggcataacat
      181 tggcagaaca acatctttaa cctttcacac cacctgccgt caggccgttg accagccagc
      241 gttgagccag caagaagacg atggtgatcg gtaatgcaga catcacggca gcggcggcaa
      301 agtcacccca caggtagttt tgcgggttga ggtattgctg catccccacg gccagggtgt
      361 agctgtttac gtcacgcagt aacagcgacg cgaccggaac ttcagtaatg gcagcgataa
      421 acgacaggat gaacaccacc gccagaatcg gtactgacag cggcaacagg acaaggcgga
      481 aggcctgcca cggtgtcgca ccatccagcg ccgccgcttc ttccagcgaa ctgtcgatgg
      541 tttcgaaata gcctttgatg gtccagacgt gcagcgcaat cccacccaga tacgcgaaaa
      601 ttacgccgcc gtgagtattc aggccaatga atggaatgta ctcacccaga cgatcaaaca
      661 acgcatacaa cgcgaccagt gaaagtactg ccgggaacat ctggaaaatc agcattcctt
      721 tcagcagcgt cgctttgcct ggaaagcgca tacgggcgaa agcgtaggcg caggtggtgg
      781 agagcgccac aatgccaatc gcggaaatcc cggcgacctt taccgagttc cacagccaca
      841 gcagtaccgg gaatggcggt ggcgtaatgc gaccatcagc ctgttccacg ctaaaaccta
      901 atgccagttt ccagtgatcc caggagattt gctccgggat caggctaccg gtcgcaaagt
      961 tcccctggcg cagtgagatg gcgacgacca tcagcagcgg gaacataatc gccgcgataa
     1021 aaagtagcag tagcaggtga gtaataaaca aacgtgcttt ttgcgatttc ggttggacca
     1081 ttgccatttt tgttatctcc cttaatcaaa cttcattcgc gtggctttca ggttcactat
     1141 cgccagcgca cccaccagca ggaagatcag cgtggctatt gctgctgcca gaccgaagtc
     1201 ctgacccccg ccgccttcaa aagcgatgcg gtaggtgtag ttaacaagca ggtcggtata
     1261 accggctggc gtggtcgtgc caagacgatc cgggccgccg ttggttaaca gttgaatcag
     1321 cacgaagttg ttaaagttaa aggcgaagct ggcgatcatc agcggcgtca gcggtttaat
     1381 cagcagcggc agcgtaatct taaagaagtt ctggaaggga cctgcaccat ccattgctga
     1441 ggcttcatac aaatcgtccg gaatcgcttt cagcaagccc atgcagagga tcatcatgta
     1501 cggataaccc agccaggtat tgacgataat cagcatcgtt cgggcggtgg tcggatcgct
     1561 gaaccaggcg ggcttcacgc caaacagcgc gctcaacatc atgttgatct caccgaagct
     1621 ctggttaaac aaccctttga aaatcaagat tgaaatgaat gatggcaccg cgtagggcag
     1681 aatcagcagg acgcgataga ccgctttgcc gcgcaacgct tcccactgca ccagacacgc
     1741 cagaaccatg ccgaccgcca ccgttaaaaa gacagtgatc agcgagaaca ccacggtcca
     1801 gacgaaaatg gcgaggaacg gtttctgaat gccttcgtcg gtgaagacgc gggtaaagtt
     1861 tttccagcca gtagtcacgg tgtaaccggg gcttagcttt tcatcgcccc agttgccgtc
     1921 ggcggtaatg gactggtaaa agccaatttg gttattcgga cgatatttca cgccgctctg
     1981 attattcgtc aacgtgccgt caccgtcgag tgtgtagagc ggctgcgtgc cagaaaactg
     2041 gcgcagggag ctcatcatca ctttgttgcc atccggcaga atggcggtaa tgtcactcag
     2101 cgcctgacga ttctgggtaa tcacgcgcag atttgcgcgt tcgccttcgg gctgggcggt
     2161 cgtttctttc agttgcagtt tttgctcgcc gccaaattta aaagcgtcgg agaggtaatt
     2221 tttgccggtt tcgccgtcgc tgagggctag ttgccactca tcgcccgccg ggtaaagacc
     2281 aaaattatag gttttgcctg cttgccagga gcgatctaac aacacttcct gcgcacgttc
     2341 aaaagtcagc tggttagtgc tgctgtagtt ggtgaaggca atggcgatgg tgcagaccag
     2401 agggaagagg acgaataatc ccattccggc cattcccggg taaacatagc gccaggcgta
     2461 ggctttacga ttggcgaaaa tatacagccc cgctgaactc aatatcagcg tggtaatggc
     2521 aaacaggtat tccccttgtg cgtacattaa aacaacaagg taacccacca gcaggccgag
     2581 cagacctagc actgaccatt tcagcgcgtc gctttgccac caatgtttct ttttaatgac
     2641 atccatgggg ttcttcctca ttccaggacg gataaggctt tcacgcctta tccgacaaca
     2701 actgcctgat gcgacactga tgcgtcttat caggcctaca tacgtttcgg ttttgtaggc
     2761 cggacaaggc gttcacgccg catccggcat ttcacagcat tacttggtga tacgagtctg
     2821 cgcgtctttc agggcttcat cgacagtctg acgaccgctg gcggcgttga tcaccgcagt
     2881 acgcacggca taccagaaag cggacatctg cgggatgttc ggcatgattt cacctttctg
     2941 ggcgttttcc atggtggctg caatacgtgg atctttcgcc aactcttcct cgtaagactt
     3001 cagcgctacg gcacccagcg gtttgtcttt attaaccgct tccagacctt cgtcagtcag
     3061 cagatagttt tcgaggaact ctttcgccag ctctttgttc ggactggcgg cgttaatacc
     3121 ggcgctcagc acgccaacga acggtttaga tggttgaccc ttgaaggtcg gcagtaccgt
     3181 tacaccataa ttcactttgc tggtgtcgat gttggaccat gcccacgggc cgttgatggt
     3241 catcgctgtt tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt
     3301 catgtgtttg tttttaatca ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt
     3361 atccacgccc acgtctttaa tgtcgtattt gccattttca tacttgaacg cataaccccc
     3421 gtcagcagca atcagcggcc aggtgaagta cggttcttgc aggttgaaca tcagcgcgct
     3481 cttacctttc gctttcagtt ctttatccag cgccgggatc tcttcccagg tttttggtgg
     3541 gttcggcagc agatctttgt tataaatcag cgataatgct tcaacagcga tcgggtaagc
     3601 aatcagcttg ccgttgtaac gtacggcatc ccaggtaaac ggatacagct tgtcctggaa
     3661 cgctttgtcc ggggtgattt cagccaacag gccagattga gcgtagccac caaaacggtc
     3721 gtgcgcccag aagataatgt cagggccatc gccagttgcc gcaacctgag ggaatttctc
     3781 ttccagttta tccggatgct caacggtgac tttaattccg gtatctttct cgaatttctt
     3841 accgacttca gcgagaccgt tatagccttt atcgccgtta atccagatta ccagtttacc
     3901 ttcttcgatt ttggcgagag ccgaggcgga aaacatcatc gtcgttaatg cggataatgc
     3961 gaggatgcgt gcacctgttt ttattttcat aatctatggt ccttgttggt gaagtgctcg
     4021 tgaaaacacc taaacggact ctagtttctt tatacggcaa cctctttcca tcctccttgc
     4081 ccctacgccc caccgttgct ttgtgtgatc tctgttacag aattggcggt aatgtggaga
     4141 tgcgcacata aaatcgccac gatttttgca agcaacatca cgaaattcct tacatgacct
     4201 cggtttagtt cacagaagcc gtgttctcat cctcccgcct cctcccccat aaaaaagcca
     4261 gggggtggag gatttaagcc atctcctgat gacgcatagt cagcccatca tgaatgttgc
     4321 tgtcgatgac aggttgttac aaagggagaa gggcatggcg agcgtacagc tgcaaaatgt
     4381 aacgaaagcc tggggcgagg tcgtggtatc gaaagatatc aatctcgata tccatgaagg
     4441 tgaattcgtg gtgtttgtcg gaccgtctgg ctgcggtaaa tcgactttac tgcgcatgat
     4501 tgccgggctt gagacgatca ccagcggcga cctgttcatc ggtgagaaac ggatgaatga
     4561 cactccgcca gcagaacgcg gcgttggtat ggtgtttcag tcttacgcgc tctatcccca
     4621 cctgtcagta gcagaaaaca tgtcatttgg cctgaaactg gctggcgcaa aaaaagaggt
     4681 gattaaccaa cgcgtcaacc aggtggcgga agtgctgcaa ctggcgcatt tgctggatcg
     4741 caaaccgaaa gcgctctctg gcggtcagcg tcagcgtgtg gcgattggcc gtacgctggt
     4801 ggccgagcca agcgtatttt tgctcgatga accgctctcc aacctcgatg ctgcactgcg
     4861 tgtgcaaatg cgtatcgaaa tctcccgtct gcataaacgc ctgggccgca caatgattta
     4921 cgtcacccac gatcaggtcg aagcgatgac gctggccgac aaaatcgtgg tgctggacgc
     4981 cggtcgcgtg gcgcaggttg ggaaaccgct ggagctgtac cactatccgg cagaccgttt
     5041 tgtcgccgga tttatcggtt cgccaaagat gaacttcctg ccggtaaaag tgaccgccac
     5101 cgcaatcgat caagtgcagg tggagctgcc gatgccaaat cgtcagcaag tctggctgcc
     5161 agttgaaagc cgtgatgtcc aggttggagc caatatgtcg ctgggtattc gcccggaaca
     5221 tctactgccg agtgatatcg ctgacgtcat ccttgagggt gaagttcagg tcgtcgagca
     5281 actcggcaac gaaacccaaa tccatatcca gatcccttcc attcgtcaaa acctggtgta
     5341 ccgccagaac gacgtggtgt tggtagaaga aggtgccaca ttcgctatcg gcctgccgcc
     5401 agagcgttgc catctgttcc gtgaggatgg cactgcatgt cgtcgactgc ataaggagcc
     5461 gggcgtttaa gcaccccaca aaacacacaa agcctgtcac aggtgatgtg aaaaaagaaa
     5521 agcaatgact caggagatag aatgatgatt actctgcgca aacttcctct ggcggttgcc
     5581 gtcgcagcgg gcgtaatgtc tgctcaggca atggctgttg atttccacgg ctatgcacgt
     5641 tccggtattg gctggacagg tagcggcggt gaacaacagt gtttccagac taccggtgct
     5701 caaagtaaat accgtcttgg caacgaatgt gaaacttatg ctgaattaaa attgggtcag
     5761 gaagtgtgga aagagggcga taagagcttc tatttcgaca ctaacgtggc ctattccgtc
     5821 gcgcaacaga atgactggga agctaccgat ccggccttcc gtgaagcaaa cgtgcagggt
     5881 aaaaacctga tcgaatggct gccaggttcc accatctggg caggtaagcg cttctaccaa
     5941 cgtcatgacg ttcatatgat cgacttctac tactgggata tttctggtcc tggtgccggt
     6001 ctggaaaaca tcgatgttgg cttcggtaaa ctctctctgg cagcaacccg ctcctctgaa
     6061 gctggtggtt cttcctcttt tgccagcaac aatatttatg actataccaa cgaaaccgcg
     6121 aacgacgttt tcgatgtgcg tttagcgcag atggaaatca acccgggcgg cacattagaa
     6181 ctgggtgtcg actacggtcg tgccaacctg cgtgataact atcgtctggt tgatggcgca
     6241 tcgaaagacg gctggttatt cactgctgaa catactcaga gtgtcctgaa gggctttaac
     6301 aagtttgttg ttcagtacgc tactgactcg atgacctcgc agggtaaagg tctgtcgcag
     6361 ggttctggcg tcgcgtttga taacgaaaaa tttgcctaca atatcaacaa caacggtcac
     6421 atgctgcgta tcctcgacca cggtgcgatc tccatgggcg acaactggga catgatgtac
     6481 gtgggtatgt accaggatat caactgggat aacgacaacg gcaccaagtg gtggaccgtt
     6541 ggtattcgcc cgatgtacaa gtggacgcca atcatgagca ccgtgatgga aatcggctac
     6601 gacaacgtcg aatcccagcg caccggcgac aagaacaatc agtacaaaat taccctcgca
     6661 caacaatggc aggctggcga cagcatctgg tcacgcccgg ctattcgtgt cttcgcaacc
     6721 tacgccaagt gggatgagaa atggggttat gactacaacg gcgatagcaa ggttaacccg
     6781 aactacggca aagccgttcc tgctgatttc aacggcggca gcttcggtcg tggcgacagc
     6841 gacgagtgga ccttcggtgc ccagatggaa atctggtggt aatagcaaaa cctgggccgg
     6901 ataaggcgtt tacgccgcat tcgacaacca acgcctgatg cgacgcttgc gcgtcttatc
     6961 aggcctacaa cggctgtcaa atgtaggccg gataaggcgt ttacgccgca tccggcataa
     7021 aaacaggttg tcattatctg aaaggggcga aagcccctct gattatcggg tttagcgcgc
     7081 tattgcctgg ctaccgctga gctccagatt ttgaggtgaa aacaatgaaa atgaataaaa
     7141 gtctcatcgc cctctgttta tcagcagggt tactggcaag cgcgcctgga atcagccttg
     7201 ccgatgttaa ctacgtaccg caaaacacca gcgacgcgcc agccattcca tctgctgcgc
     7261 tgcaacaact cacctggaca ccggtcgatc aatctaaaac ccagaccacc caactggcga
     7321 ccggcggcca acaactgaac gttcccggca tcagtggtcc ggttgctgcg tacagcgtcc
     7381 cggcaaacat tggcgaactg accctgacgc tgaccagcga agtgaacaaa caaaccagcg
     7441 tttttgcgcc gaacgtgctg attcttgatc agaacatgac cccatcagcc ttcttcccca
     7501 gcagttattt cacctaccag gaaccaggcg tgatgagtgc agatcggctg gaaggcgtta
     7561 tgcgcctgac accggcgttg gggcagcaaa aactttatgt tctggtcttt accacggaaa
     7621 aagatctcca gcagacgacc caactgctcg acccggctaa agcctatgcc aagggcgtcg
     7681 gtaactcgat cccggatatc cccgatccgg ttgctcgtca taccaccgat ggcttactga
     7741 aactgaaagt gaaaacgaac tccagctcca gcgtgttggt aggaccttta tttggttctt
     7801 ccgctccagc tccggttacg gtaggtaaca cggcggcacc agctgtggct gcacccgctc
     7861 cggcaccggt gaagaaaagc gagccgatgc tcaacgacac ggaaagttat tttaataccg
     7921 cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc gttaaaactg cttgatgaag
     7981 ctgaacgcct gggatcgaca tctgcccgtt ccacctttat cagcagtgta aaaggcaagg
     8041 ggtaattacg ccccacagtg ctgattttgc aacaactggt gcgtctcctg gcgcaccttt
     8101 ttttatgctt ccttcctggg atatgagcga ttttttatag taactcactt cttcttcact
     8161 aagaatatcc attatctcaa tgccttatca gagattcttt tcctttcgcc ggtagtgtct
     8221 ggacattcag gctacttttc caggttattt tatttctgtt atgcagaggt tttatgataa
     8281 gtcatatcct aaattctggc ggcaataact ctttgatgaa acatgatgtg gtgcaaggaa
     8341 ataatatagt agatcttgat ttactacgta atttcaatgg ggtgccaggt ttaaacagag
     8401 ataactttat ttatatcagc aatatttttt caaatataaa acaacggaac gaaaaaatca
     8461 tgcaataaat atgtttcgtg aagtctcaat cagtaatg
//
LOCUS       DAPHJE010000155         8433 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPHJE010000155 DAPHJE010000000
VERSION     DAPHJE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.157"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(15..2000)
                     /gene="yjcS"
                     /locus_tag="RH222_003971"
     CDS             complement(15..2000)
                     /gene="yjcS"
                     /locus_tag="RH222_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV3798911.1"
                     /translation="MNNSRLFRLSRIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(2273..3202)
                     /gene="alsK"
                     /locus_tag="RH222_003972"
     CDS             complement(2273..3202)
                     /gene="alsK"
                     /locus_tag="RH222_003972"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDV3798912.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPQNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(3186..3881)
                     /gene="alsE"
                     /locus_tag="RH222_003973"
     CDS             complement(3186..3881)
                     /gene="alsE"
                     /locus_tag="RH222_003973"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDV3798913.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(3892..4872)
                     /gene="alsC"
                     /locus_tag="RH222_003974"
     CDS             complement(3892..4872)
                     /gene="alsC"
                     /locus_tag="RH222_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDV3798914.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4851..6383)
                     /gene="alsA"
                     /locus_tag="RH222_003975"
     CDS             complement(4851..6383)
                     /gene="alsA"
                     /locus_tag="RH222_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDV3798915.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLVHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(6510..7445)
                     /gene="alsB"
                     /locus_tag="RH222_003976"
     CDS             complement(6510..7445)
                     /gene="alsB"
                     /locus_tag="RH222_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDV3798916.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(7504..8394)
                     /gene="alsR"
                     /locus_tag="RH222_003977"
                     /gene_synonym="rpiR"
     CDS             complement(7504..8394)
                     /gene="alsR"
                     /locus_tag="RH222_003977"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDV3798917.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
BASE COUNT         2048 a         2135 c         2051 g         2199 t
ORIGIN      
        1 aaaagcgagt gacattaatt tggggttacg atattcaccc acaaatcgaa attatccaga
       61 caggcgataa tttcttccag tttcgcgcca ttgccaataa ttttggcttt cttcgctttt
      121 accagatccg ccattttggc ttgcccggtc agcacggcgt gcagatcttc acggctgatg
      181 tagaaagagg cgtcagcttg ggattgcagt gttttacggt agttaagcac gctatcgttc
      241 agcgtcaggt tgaggttatc gccattgctc atattgaagt tcaggctgat atttttaccc
      301 gcggctttcg cgctatcgag gcgaacggac ataaagtcga acagcatttc gaccgacatc
      361 ccgcgaatgg tgtccgggga accggtggtg ccgtggctga acttatgcac cccttcgcgc
      421 agctctttcg cgccggtcag gtagaaaccg cgccaggtgg cggactcggc ctgatagccc
      481 agctgttcaa agttattcgc ttgcagattc ttcgcgacct ggtcacccgg gttggcggca
      541 atcacctgtt tcagcagttc tgccgaccag cggtaatcac cttgcttgtt cgcttcttgc
      601 gccaggttga tgacacgggc agaaccgccc agcgcctgca cgtaacgttt acccatctcc
      661 acctgaccat acggatgcag gttagccggg ttaccgtcgt aatagccaag atagaagtta
      721 tacaccgcgc gggcgttgtg gctgacagaa ccgtaatagc cacggctggc ccagttattg
      781 gcaagtgcag gcggcagttt aatcatgtcg ccgatttcat tcatggtgta gccctggttc
      841 gccaggtgca gggtctggtc gtgaatgtac ttgatagtat cgcgatattt accaatataa
      901 tcattgatat gcttattgcc ccagaccggc caggtgtgcg gcataaacag gacttccgcg
      961 tcgttacccc acatatccag cgtttcgttc agatactcgg tccacttgct ggtgtcgcgg
     1021 gttttcgcgc cgcgtagagt gtagaagttg tgcagggtat gcgtggcgtt ctcggcggta
     1081 cacagcgctt tcagggccgg aatatagaag tgcatttcgg ctggtgcttc gctacctggg
     1141 gtcatcagga agtcaaactc caggccgtcg ataatcatct tctcaccggt tttggtgatg
     1201 gttttgttcg gtgcgatgat gctcgggtag cccgatgcca gcgttacgcc aagaccattg
     1261 ccgatgttac cctgagggtt atgcgccagc agcaggccgt aggagtaaag tgcacggcgg
     1321 ctcataatat tacccgccag tacgttttcg ctgatggctt cgtccataaa gccagcaggg
     1381 gcgataacct gcactttacc ggatttcaca tcggcttcag agataatgcc tttcacgccg
     1441 ccatagtggt cggtatggct gtgggtataa ataaccgcaa caatcggttt ttgcgggcga
     1501 ttttggaagt aaaggtcaag ggcggctttt gcgctcggtg gcgtcaccag cgggtcgatg
     1561 acaataatcc cggtatcacc ttcgataaag gtgatgttgg agatatcctg accgcgaacc
     1621 tggtacattc tgtcggtgac tttaaacagg ccggaaatac cgtttaactg cgactgacgc
     1681 cacaggctag ggttaacggt ttcgggcgcg ggcgcattga tatcaaattt ataatcttcc
     1741 ccgcgataat atggtttgcc attagcatcg cgcagaatac cttcatccag cagcggagcg
     1801 ataaatccac gctgagcgtc gtcaaaatcc tgacgatcgc taaacggtaa tgatttggcg
     1861 taattctgat taacctgttg ggtatattga gtggcggctt tcgcttcatc tgttgcgtaa
     1921 gcggtattta ccatcatgcc gctggcggca gttaacgcaa taataattct gctcaaacgg
     1981 aataaccgag agttattcat ataaatctcc atttatcatt tatgtcgtaa atatgtaatc
     2041 catgcaaaat acgtcattta aagctggggt tatcataatt gggaatttgc gactttaata
     2101 agtggaagtg tgagctgaac gatccatttt attaggcatt tgtgctgaag aggaaaagtt
     2161 acagtaaaac tatcattgca gaaaataata tgagccgttt tatgtgttta ttatatatat
     2221 tgtacctgtc gttggcacag cgggacattg cgctactgtg ccaacgcttt actcatgggg
     2281 ctttagcaca ggactgtggc aaaaaatgtt gatgcgccaa tattgctgca ccctgagcgc
     2341 cattaaagtc agaagatgag gcggcaataa agcgcacgac ctgatgcggc agtggacggc
     2401 gcaggtactt ttgggtcatg gcaatgagag tctcgcgtgg gaaggcgggc atatccatca
     2461 cgccaccgcc cagaatcacc gcatcgggat cgaacagatt aatgctggtg gcaatggccc
     2521 gtgccgcgtt ttcaagcaga ctctggacga aaggggcgtt ttccgcatgg acgaaaaggt
     2581 cgctcaatgg gtaattttgg ggctgttgtt cgtaccagcg tcttagcgcc attccagagc
     2641 aattggtttc caggcaccca ggattgccac acgcgcagtg ttgggtcata tctcccaggg
     2701 ggatatgacc cagttcgcct gccacaccgt gtgcacccgt ccacggcgca ccgttcatcc
     2761 acactgcgaa ccccatcccc gtaccgagat aggccgccag aaccagttgt tgcgtaaggc
     2821 ggttttctac tacgtcccag gagagttgca ggttaacgtc gcgggaaaac tcaaccggac
     2881 aattcagcgt attttcgagc ttatcggcga gatcatataa atccgccgct gttaacggca
     2941 ggttaggcgt agaaattatg gtgcgtttat ctttactgac cagcgccgga aatcccatca
     3001 ccagaccatg acagcgagcg ttaaagcgcc tgagttgctc gtcaatcatt tcgccgatac
     3061 ccgacaccag gccgggagca atgacttctg cggtccgctt tttttcgcag tgtagcgttt
     3121 caccttctgc tgtccgcaga caaaagcgga tatgcgttgc ccccatatcc acgcccgcta
     3181 cgacgttatg ctgtttttgc atgaggctgt acctcgcttt ttgcagccag aatctgcgtg
     3241 gtcataattc tccatgcttc gtcgatattt tccgcatgat taaacaggcc ggaagtgccg
     3301 acgataaaga catccgcccc tgccgccatc agtttttcgt aagttgcctg gttgcaggaa
     3361 ccgtccacct caatttcgta ctccagacct tctcgttcac gccatgcctt cagttcggca
     3421 agtttatcca gcatttcagg aatgaacggt tgtccggcaa agccgggatc gacagtcatg
     3481 accgtaattt tatcggcctt atggatatag tatttcatgg cctcaactgg tgtctccggg
     3541 ttaaggatca gccccacttt catgtcatga cagcggattt catcaatcag gcggaacgcc
     3601 tgaccgttga tagtttccgg atgcaaagtg atgaaatcag ctcccgcacg cgccagttga
     3661 gcaatgtaat cctgcggccg cgtcaccatc agatgacagt cgagcggttt agttgccagt
     3721 tttttaacct gacttacgaa gaacggtgag agtgtcagat tggggacaaa gtgaccatcc
     3781 atgatatcga tgtgaaagta atcggcatgg ctgtcgataa attcgatctg ttctttaaat
     3841 ttcagcagat ccatacacat taacgagggg gagattttca tgttcaattc cttacttact
     3901 gataagacgg tcaagggcga cagccgcgat aattaatccg cccatcacca ccagttggta
     3961 ataggtttgt acctgcaaaa tattcagacc gttgttgatg gtgccgatga tcaacccgcc
     4021 aatcaccaca gagaaaatgc gccccttgcc gccgaagaaa ctggtgccgc caatgatggc
     4081 gctggcaatg gcataggttt caaaacccat accggcaagc ggttctgcgg caccgagtcg
     4141 tgcagttgag acgacgcctg ccagacctgc acaaacaccg gagatgataa acaccaccag
     4201 gatgtggaat ttcacgtcaa tcccggaata gaacgccgaa tttttgttgc cgcccagtgc
     4261 gtagatgttg cgcccgagcc gcatacgcgt tgtcagaaac caaaggatga gcgcgacaat
     4321 tagcgagaag ataacgggga caggtatccc aattacgctg gcggcaaaga agttcacgaa
     4381 gtcaaaggag aagccgtata ccgagttggc atcggagatc accagcgtga tcccacggaa
     4441 aatcgcgttg gtgccaaggg tgatgatgaa cgggtgtagc cccgtccagt tgaccaggca
     4501 gccgttgatc gcccccagtg cgccgccaac cagtacaccg ccaatcatcg ctgcgagaaa
     4561 cgggtcaaca cctgccaaca tcagtttggc ggtcaccata ccggaaagcg ccagaatcgc
     4621 gccaaccgag aggtcgatac cagcgaccag gatagcgaaa aactcgccca tgccgatcaa
     4681 taccgtcacg gagctttgaa caaaaatctg ggtaatatta ttggtggtca gaaaatattc
     4741 tggcgacagc gaaccaaaaa tggcgacgat gatcgccagg ataaaaaagg tgccgtattt
     4801 atcccagaac agcgcaaagt tgaacggttt cttctcgctc gcttcgcttt ttactcttgt
     4861 ggtaaagccc atgccataat ctcctcttcg ctcatgtcat cgcgattcgt caggatttgc
     4921 gtcagtcgtc cttcgcagaa cacggcgatg cggtcgcaga cggtgataat ttcaggtagt
     4981 tcagatgaca ccatcaggat gacttttccg tcgtccgcca gttggcgcat cactttgtaa
     5041 atttcggctt tcgcgccaac gtcgatgccg cgggtaggtt catcgaaaat aatcacttcc
     5101 gggcaacagc acagccattt ggagatcagg actttctgct gatttccccc ggagagttcg
     5161 gtgatattct ggtttaccga atgacatttc agcgccagca gttcgcgttg attttcagcg
     5221 gtacgttgct cgtcaacttc atgaaacaag cccatcgcgc ctttatagcc gccgtctttc
     5281 agactgcggc tgatcgccat gttctgagcg atggaaaagt tgggaaaaaa accgttatcc
     5341 cggcggcttt cagtgatgta agccatccct tttttcactg catccagggg ggaacgtgga
     5401 gagatatctt tgccattaag acggatttct ccgccagcgc gtttatcaac gccaaacaga
     5461 caattcatca gttcagtacg tccggaaccg accagcccgg caaagcctaa tatttctccc
     5521 cggcagacgc taaatgagat atcccggacc ttttttctgt cacggctggt gacgttgcgc
     5581 acctcaaaaa ccgtatcgtg cacaaggttg ctgacattct ccttcatcgc gttaaaacgg
     5641 ttttgcagtt cgcggcctac catcagacgg acgatatcgt catttgacac atcgcttacc
     5701 atgccgctgc aaacgctgct gccgtctttc atcaccgtat agcggtcgca aatacggcga
     5761 atttccgcca acttatgcga gatatagacg atggccgtac cctctttacg caactgattc
     5821 atgatcagaa acagatagtc cacctcttta ttggtgagtg aggaggtggg ttcatccatg
     5881 atgatgactt tggcatcgag catcagcgtt ttggcaattt ccagcatctg cttgtggctg
     5941 atagatagat tcgccacttt ctcatctaaa tcaactttca agcccacgcg taataacatc
     6001 atggcggcgc ggacacgcat ttctcgccag tcgataatat tgacgccaca gatttttttc
     6061 gtcagatgac gaccaatata taaattttcc agtacggtta attcatcaat aacgctgagt
     6121 tcctgataaa taatcccgat accgagttgt gccgctaatt tatgatccag cttgttatag
     6181 ctaatgttat taatggtaat ggtgcctttg gtcggctcat gtattccgga taaaaccttc
     6241 attagcgtgg atttacccgc gccattttct cctagtaatg catgtatttc accaggataa
     6301 accgttaaat taaccgactt taatgcgtga accggaccaa aggacttgcc gatccccgcc
     6361 atcgatatat atggcgtggc cataaataac ctctctgcgc gaaatatcgt attattgcct
     6421 gatggctaca tttatcaggt ctacgttcag gttattttgt tggccggata agacacttca
     6481 ttagcatcat catccggcat cattcagttt tattgagtga ccaggattga atcgaccagt
     6541 ttaaattccg gtgctttatc cagcgggata accttgccgg atttctcagc gttaaccatc
     6601 agcttcagac ccgttgcgcc aatatctgcc gggttctgcg caaccgtcgc ggtcatttgt
     6661 ccggcttcca ccattttgcg ggcttccgga attccatctg taccgacgac caatattttg
     6721 cccgtttttc cggcgtttgc gactgcctga gcaacaccca ttgccatcgt gtcattcgcg
     6781 caatagatcg ctttaatatt cggattacgt tgcaacacgt tagtggcgac atccagtgct
     6841 ttaatgcggt cccagtcagc gggctggctg gcgacaagct tgatctggct tgcttttttg
     6901 aaggcttcgg tggcaccatt acgacgcgct tcaccggagg cgttaccggc tttaccctca
     6961 atgattgcga cttcaccacc ttcagcgccc aatttgtcaa taatgaacga cgcgcctttc
     7021 gccccgacag caacgttatc ggtggtgaca aaagcttcca cattgccgcc agcttttttc
     7081 agattatcca tgtcgatttt ttcatcgaga ttaaccagat aaatgccttt tttccatgcg
     7141 cgggcgacag gcatgaccag attcactgag gataatggag cgaaggcgat acctttgtaa
     7201 tttttattac tgagatcttc aaataactgc aattgagatt gaaaatcgcc ttctgaagga
     7261 gaggcaaaaa tatcaacgct gacgcccagt gtttttgctt catcttcaat gccttttttc
     7321 atatctaccc aaaatgggtt ggagagggtt ttcaatacga cagcatattc ggcggcagca
     7381 aaagcgctgg ttgacaacat taagcccacg agtgtgccgc tgaaatattt cagatattta
     7441 ttcataataa tgttctcacg gtaaggggac aggtaactcc gcgatggcgg aattaccttt
     7501 ttcttatttc agcgcgcctg gtgagaagaa atcaacaatt gcgccggttt tttgcatatt
     7561 aatattagct tgttcaatgt tgagctgggc gacagagaca aaaaaagcgt ccagcaaagt
     7621 tagttgtaat attcttgccg aggcattacg acctaataac ggcgtttccg gggctggtga
     7681 gcaaataata taatcggcca gtttcgctat cggtgaatgg tagctatggg ttatacaaat
     7741 aatctttgcc ccgttctttt ttgccagttc tacggccgct tttacatcac tggttcgccc
     7801 ggaatgggtc actaccagca caacatctcc ttcctgtaac aacgaagcgg acatcatcat
     7861 gatgtgagca tcaggatagg cctgacagcg tacgccaatg cgcaagaact tgtgctgtac
     7921 atcagcacag atagcatttg atcctccggc accgtacaaa tcccgctgtc tggcctgata
     7981 gaaaaagcgg gcggcacggt ggatctcatc aacgttgacg atcgactgac cttccataat
     8041 ggtgcgtaaa gtgatgttaa ataccttatt caccacatcc tgcggcgctt catcaaaagc
     8101 caactcggaa ggcaatacct gttctgactg agaaaaataa tcttccagcg cactgcgtaa
     8161 gttacgaaag ccgctaaacc ccagcagctt tgatacctta actatcatcg cttcagatac
     8221 cgccagagct tctgcgacat ctttaattgc gggtgcacaa ctcaggttac cgggtttgag
     8281 taaccactct acgatgcggc tttcattttc tgtcattcct tcctgcttca ttcgcaggta
     8341 aggcgctaac cctataccgt tcggaagcgc tgaatcaaac tctgactggc tcatcacgtt
     8401 ctcttgtcct tgaattgaag acgccactat atc
//
LOCUS       DAPHJE010000156         8336 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPHJE010000156 DAPHJE010000000
VERSION     DAPHJE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.158"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..234
                     /locus_tag="RH222_003978"
     CDS             <1..234
                     /locus_tag="RH222_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798918.1"
                     /translation="VDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLT
                     QETLFSSKSQDKHALFPIFTASWRAHRIMNKGV"
     gene            457..954
                     /gene="ubiC"
                     /locus_tag="RH222_003979"
     CDS             457..954
                     /gene="ubiC"
                     /locus_tag="RH222_003979"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV3798919.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            967..1839
                     /gene="ubiA"
                     /locus_tag="RH222_003980"
     CDS             967..1839
                     /gene="ubiA"
                     /locus_tag="RH222_003980"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV3798920.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="RH222_003981"
     CDS             complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="RH222_003981"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV3798921.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4588..4956
                     /gene="dgkA"
                     /locus_tag="RH222_003982"
     CDS             4588..4956
                     /gene="dgkA"
                     /locus_tag="RH222_003982"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV3798922.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5066..5674
                     /gene="lexA"
                     /locus_tag="RH222_003983"
     CDS             5066..5674
                     /gene="lexA"
                     /locus_tag="RH222_003983"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV3798923.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5747..7072
                     /gene="dinF"
                     /locus_tag="RH222_003984"
     CDS             5747..7072
                     /gene="dinF"
                     /locus_tag="RH222_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV3798924.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSKVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7188..7397
                     /locus_tag="RH222_003985"
     CDS             7188..7397
                     /locus_tag="RH222_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV3798925.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7439..7954)
                     /gene="zur"
                     /locus_tag="RH222_003986"
     CDS             complement(7439..7954)
                     /gene="zur"
                     /locus_tag="RH222_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV3798926.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1903 a         2028 c         2273 g         2132 t
ORIGIN      
        1 gtcgatgatg ggcctgttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct
       61 acaaaagata ccaattggaa ttccacgtat atctacaaat ccagacatga gatgttgcct
      121 gttaatctta ctcaggaaac acttttcagc tccaaatctc aggataaaca tgcgcttttc
      181 cccattttta ctgcgagttg gcgagctcat cgtataatga ataagggtgt ttaagtaaag
      241 gaaaacatca ccgttcctgg catcctgggc ggtgatgccc ctatggttgc cctctccagc
      301 gcgggcatcg gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt
      361 ttttgttgcg tattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga
      421 tacaatgcct ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa
      481 ctgcgtgcgc tgcgctattt taaagagatc cctgccctgg aaccgcaact gctcgactgg
      541 ctgttgctgg aggattccat gacaaaacgt tttgaacagc agggaaaaac ggtaagcgtg
      601 acgatgatcc gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg
      661 ccgaaagagt ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg
      721 cttgccggtc gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa
      781 aaattgggta aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac
      841 tttattgaga taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc
      901 ggtaaaccgc tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa
      961 aaaaatatgg agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt
     1021 acggataagc caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg
     1081 gcgacaccgg gcgttcctca gctctggatc ctggcggtat ttgtcgcggg tgtctggctg
     1141 atgcgcgctg ctggatgtgt ggtgaatgat tatgctgacc gaaagtttga tggtcatgtt
     1201 aagcgcacgg cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg
     1261 ctgtttgtag tgctggtact gatttcgttt ttactggtac tgacgctgaa tacgatgacc
     1321 attctgttgt cgattgccgc tctggcgttg gcgtgggtgt acccgtttat gaagcggtat
     1381 acccatctcc cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt
     1441 gccgctgtga gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc
     1501 tgggcggtgg cttacgacac gcagtatgcg atggttgacc gcgatgatga tgtgaagatt
     1561 ggcattaaat ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg
     1621 cagattggcg tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga
     1681 tattactggt caattgtggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc
     1741 aaccgcgagc gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta
     1801 ctgtttttag gactggcaat gagttactgg catttctgat gatgtaaaaa agccggatga
     1861 tcatccggct ttcttctggg ttgccttatg cgcggcgctt ctcaggccta cacaacacat
     1921 cgcaatttat tgaatttgca gattatggaa ggccggataa ggcgttttcg ccgcatccgg
     1981 caattctctc tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg
     2041 atgtaatcaa ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcgtcgc
     2101 cgctatcgct gatataccct tcatcacgca gcgtcagcac cagagaactg aacaccgcct
     2161 tgtcgaagaa ctccggcgcg ttgatgccgt gcagcacgga gagacgttgc gcgacggtgc
     2221 ggctctcttt ctccagcgta ccgcggttga tcgacgggtt ggcactcaac aaccagaagg
     2281 tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt agggtgcgag
     2341 aatgcgccgg gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca
     2401 tctcatttgc cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca
     2461 gctccgcttt cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga
     2521 tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa
     2581 tgttgttgcg atagtaggtc atcagcaccg cttgctcgcg tggcagaatg atgatgtcgc
     2641 cgatagtgtc tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc
     2701 tggcgctggc tgaaggaacg gtagagtccg tggagtaagg tacgttgcgc atcagatcca
     2761 ggtagcagtt gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca
     2821 gaagcgcagt acaacacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca
     2881 tcagatcggc agcaatatta ttgaccgtcg gcgttaacca ggccggacgc accgcttcga
     2941 tgggatcgat agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca
     3001 ttggttcacc gaagttgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca
     3061 gcatctgcgg caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac
     3121 ccacttccat gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc
     3181 cgccacgcag catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac
     3241 gccccgtgcg ggaacgaccg ccttccacga agtactcgac ggaataacca cggctgaaca
     3301 gttcgccgag atactcacgg aaaacggtgg aataaagttt attgccttta aaagtacggc
     3361 gaataaagaa cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga
     3421 tcccggcggc gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagtagca
     3481 ggtagtccat gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca
     3541 gctggcgaac gcgctcggcg ttatggacgt tgatgccctg ataaagtcgg ttccaggtga
     3601 agcccagaat gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt
     3661 ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt
     3721 catcttctac cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac
     3781 gagcaggcag acgtgggccc acagcagcca gacgttgacg ggcaaagtgc atacgcgcca
     3841 cgcgcgccag tttctgtgcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca
     3901 gcgaaactga cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acggcgaaaa
     3961 atttctgtac gccgttaagc atacgcagcg gcgggtttac ttcgcctttt tcacgccccg
     4021 gcgcgcgacc aaacatcacc gatactggca ccatctgcac atccagattt gggttgctgc
     4081 ggtgcaaatc aagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg
     4141 tgaacacacg cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt
     4201 ccagcggctc taacgggtca ggcaagtcat gagccagaca ctgggcgcgc aacgtcagca
     4261 aatctgcttt cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca
     4321 gttccggggc aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat
     4381 tcagtaattt gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta
     4441 ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc
     4501 atgtttcgcg cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga
     4561 cgctcataat gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaatt
     4621 atcaaagctg ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca
     4681 ttccgtcagg aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg
     4741 gacgcaatta cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc
     4801 ctcaatagcg ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc
     4861 ggacgcgcaa aagatatggg atccgccgcg gtgctgattg ccattatcgt cgccgtgatt
     4921 acctggtgca tcctgttatg gtcgcatttt ggataaccct tccagatttc gataaatctc
     4981 tggtttattg tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata
     5041 actgtatata cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt
     5101 tgatctcatc cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc
     5161 gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg
     5221 caaaggcgtt attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga
     5281 ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca
     5341 gcatattgaa ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct
     5401 gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt atggatggtg acttgctggc
     5461 agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga
     5521 agttaccgtt aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag
     5581 cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc
     5641 ggttggggtt attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgcc
     5701 aggcgtcgcg gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac
     5761 ttcatctgat aaagcactct ggcatctcgc cttacccatg attttctcca atatcaccgt
     5821 tccgttgctg ggactggtcg atacggcggt aattggtcat cttgatagca aggtttattt
     5881 gggcggcgtg gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt
     5941 acgcatgagc accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt
     6001 agcccgtgcg ctggtgcaac cgttgctgtt ggcgttgggg gctggggcgt taattgcgct
     6061 gctgcgtaca ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcggtcct
     6121 ggaacaggcg cggcgctttc ttgaaatccg ctggttaagc gctccggcgt cgctggcgaa
     6181 tctggtatta ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt
     6241 agtggtcggc aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctgcatat
     6301 gaacgtgcag ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg
     6361 tctgctaatg gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tgctgaaaac
     6421 tgcctggcga ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc
     6481 gctgttgttg caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga
     6541 cattatcgct gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgctgga
     6601 tggttttgcc tacgcggttg aagcacattc cggtcaggca tacggtgcgc gcgacggtag
     6661 ccagttgctg gatgtctggc gggcagcgtg ccgccagtcg gggatcgtag cgctactgtt
     6721 ttcggtggtt tatttgctgg caggggaaca catcattgcg ttactgacgt cgttaaccca
     6781 gattcaggag ctggccgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt
     6841 ctggtgttat ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa
     6901 cagtatggcg gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg
     6961 taatcatggt ttgtggctgg cattaaccgt ctttctggcg ttgcgcgggc tttctctggc
     7021 ggctatctgg cggcgtcact ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa
     7081 aattctgaat aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag
     7141 aaaagtgaac gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg
     7201 aagccggcgg taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga
     7261 ccgatgatga tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg
     7321 aacgttatgg ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca
     7381 atgaatatcg ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt
     7441 aacgcggttt ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac
     7501 acgcttccac ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca
     7561 gggcaaaccc catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacact
     7621 cttctttcac cgcgccgcag cgatcgcaaa taaacatggc tgacgtatgc gtgggctggt
     7681 cgaacagatg acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct
     7741 caagcagaaa atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc
     7801 gcagcaaatc aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca
     7861 acacttccag gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct
     7921 gcgctaataa ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga
     7981 atgtccttac tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg
     8041 ttgattttct gttttgtacg taaggtgttg tgttgcttgg tcgtaccatt atcaacacga
     8101 taataattaa taattcattt ttaaataaat tgcttcaagt taactcgcta tttatttaac
     8161 tgggtttgat cgcatacctg tagttcagaa attatgagcg atttttgata gtgaccagat
     8221 ggcaaaacac ttataaggag gacacggtac gacaaaaaag gtagatgaat catgttgaag
     8281 ataatacccg gtgcaactgg atactttaat aaaacactca attcaaatca attcga
//
LOCUS       DAPHJE010000157         8329 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPHJE010000157 DAPHJE010000000
VERSION     DAPHJE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.159"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(51..257)
                     /locus_tag="RH222_003987"
     CDS             complement(51..257)
                     /locus_tag="RH222_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV3798927.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            570..659
                     /gene="kdpF"
                     /locus_tag="RH222_003988"
     CDS             570..659
                     /gene="kdpF"
                     /locus_tag="RH222_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV3798928.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            659..2332
                     /gene="kdpA"
                     /locus_tag="RH222_003989"
     CDS             659..2332
                     /gene="kdpA"
                     /locus_tag="RH222_003989"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV3798929.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2355..4403
                     /gene="kdpB"
                     /locus_tag="RH222_003990"
     CDS             2355..4403
                     /gene="kdpB"
                     /locus_tag="RH222_003990"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV3798930.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4412..4984
                     /gene="kdpC"
                     /locus_tag="RH222_003991"
     CDS             4412..4984
                     /gene="kdpC"
                     /locus_tag="RH222_003991"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV3798931.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVINIVELNLALDKLDE"
     gene            4977..7649
                     /gene="kdpD"
                     /locus_tag="RH222_003992"
     CDS             4977..7649
                     /gene="kdpD"
                     /locus_tag="RH222_003992"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV3798932.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLGSV
                     WHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYAREHNLG
                     KIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFKDKWR
                     VQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVVATVI
                     NVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYREQRT
                     RHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQGMTPW
                     DDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMIPEQQ
                     RLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVLFGQA
                     EILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLTLEEV
                     VGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAEIGID
                     AHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIVDVHG
                     GTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7646..8323
                     /gene="kdpE"
                     /locus_tag="RH222_003993"
     CDS             7646..8323
                     /gene="kdpE"
                     /locus_tag="RH222_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV3798933.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1726 a         2173 c         2441 g         1989 t
ORIGIN      
        1 aaccgccagc gtttccgctg gcggttttca atgtattcaa aactcagcaa tcaataaaaa
       61 tcaccagttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt tttcgaccag
      121 acgcgatgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc
      181 gccagaacgc ccgctgcaac cgcataagta cggcgtaaaa agataagcca tgcaggatat
      241 tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa tggcaccgga
      301 aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat
      361 ccgaccactt atttttgctt attgatggtt tatttacatt catcctgtaa ttaagttaca
      421 caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt tttatacttt
      481 ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct acccttccgg
      541 tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgataacc ggcgtattgc
      601 tggtgttttt attactgggt tatctggttt atgccctgat caatgcggag gcgttctgat
      661 ggctgcgcaa gggttcttac tgatcgccac gtttttactg gtgttaatgg tgctggcgcg
      721 tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttcccg gtacaacggg
      781 cgttgagcgc gtactttttc gcgcacttgg cgtctctgac cgtgagatga actggaagca
      841 atatctctgt gccattctcg gcctgaacat gctggggctg gcggtgctgt tttttatgtt
      901 gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt cgtgggatct
      961 ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt acagcggtga
     1021 aaccacgttg agctatttca gccagatggc tggcttaacg gtgcaaaact ttctttctgc
     1081 cgccagcggg attgcggtga tttttgccct catccgtgcg tttacccgcc agagcatgag
     1141 cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc tcgtccctgt
     1201 ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact ttcagcctta
     1261 tcaggctgtg aataccgttg aaggagcgca acagctgtta cccatggggc ctgtagcttc
     1321 tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg ccaactcgtc
     1381 gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg cgatcttctt
     1441 gatcccaacg gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc gccaggggcg
     1501 catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg tgatgtgggc
     1561 agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca tcaatatgga
     1621 aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc
     1681 ggcttcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg gtggcatggt
     1741 gccgatgtgg ctgatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt ctggtcttta
     1801 cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac
     1861 accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg cactggcgat
     1921 tctggttacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga ccgacgccgg
     1981 acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt acgccgtgtc
     2041 atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccgttctg
     2101 gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat
     2161 ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg gcacgctgcc
     2221 aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact
     2281 gacctttatc cctgccctgg cgcttggtcc agtggcggaa tatctctcct gatgatattg
     2341 agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc
     2401 aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga
     2461 tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg atggcaagcg
     2521 gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg tgggtcaccg
     2581 tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg caggccaaca
     2641 gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg aaatatggcg
     2701 ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg ctggtagaag
     2761 ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg gtcgatgaaa
     2821 gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat tttgcctccg
     2881 tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc gttaaccccg
     2941 gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga cgcaaaacac
     3001 cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt ttactggcaa
     3061 ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc agtcagcgta acggtactgg
     3121 tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca gcgatcggcg
     3181 tcgccgggat gagccggatg ctaggcgcga atgtgattgc caccagcgga cgtgcagttg
     3241 aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc acactcggta
     3301 accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg ctggctgacg
     3361 ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg
     3421 ccaagcagcg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac
     3481 cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg atccgtaaag
     3541 gttctgtcga tgccattcgt cgccatgttg aggctaacgg tggtcacttc cctaccgatg
     3601 ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg gtggtggaag
     3661 gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt attaaagagc
     3721 gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc gataaccgtc
     3781 tgactgccgc cgcgattgct gcggaagcgg gtgtcgatga ttttctcgcc gaagcgacac
     3841 cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg gtagcgatga
     3901 ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg gtggcgatga
     3961 actccggcac ccaggcggcg aaagaggcgg gcaatatggt cgatctcgac tctaacccga
     4021 ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt ggctcgctga
     4081 ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg gcggcattcg
     4141 cggcaacgta tccgcagtta aatgcgctga acatcatgtg cctgcattcg cccgactccg
     4201 caatcctcag tgcggtgatt ttcaacgcct tgattatcgt ctttttgatt cccctggcgt
     4261 taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt aacttatgga
     4321 tttacggtct gggtgggctg ctggtgccgt ttatcggtat caaagtcatt gatttactgc
     4381 tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg cattatcaac
     4441 atttatcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca ccgtactggg
     4501 gcaatggtgg tttccctggc aggccaatgg ttcgttgatt cgtgaaggtg atacggtgcg
     4561 cggttcggca ttaatcgggc agaattttac cggcaacggc tattttcatg gtcgcccgtc
     4621 ggcaacggca gaaatgccct ataatccaca ggcttctggc gggagcaatc tggcggtcag
     4681 taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg ccgctaaccc
     4741 ggatgccagc gcgagcgttc cggttgaact ggtgacggca tcggcaagcg ggctggacaa
     4801 taatatcacc ccgcaagcgg cggcctggca aatcccacgc gtggcgaaag cgcgtaatct
     4861 cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccgc tggtgaaata
     4921 tatcggccag ccggttatca acattgttga actcaatctg gcgctggata aacttgatga
     4981 ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc gcgccgcatc
     5041 gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc tgggcgatgc
     5101 tggcagaagc acagcgactg cggacgcaag ggctggatat tgtggttggc gtggtagaaa
     5161 cccacgggcg gaaagatacc gccgccatgc tggaagagct ggctgttctg ccgccaaaac
     5221 gcctggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc ctcgcccgcc
     5281 gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgcctggt tctcgtcatc
     5341 ccaaacgctg gcaggatatt gaagagctgc tggaagcagg cattgatgtt tttactaccg
     5401 tcaacgttca acatctggaa agcctgaatg atgtggtcag cggcgtcacc ggaattcagg
     5461 tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt ctggtggact
     5521 tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt gccgggcagg
     5581 cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg cgcgaactgg
     5641 cactgcgccg tactgccgat cgcgtagatg agcaaatgcg cgcctggcgg gggcatcctg
     5701 gcgaagagaa agtgtggcac acgcgcgacg cgatcctttt atgcatcggg cataacaccg
     5761 gcagtgagaa actggtccgc gcggcggcgc ggctgggtag cgtctggcac gcggtgtatg
     5821 ttgaaacccc tgccctgcat cgcttaccgg agaaaaaacg tcgggcaatt ctcagcgcct
     5881 tacgtctggc gcaggagctg ggcgcggaga cggcaacact ttctgatcca gcggaagaga
     5941 aagcggtagt gcgttatgcc cgtgaacata atctcggcaa gattattctc ggtcgcccag
     6001 cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg actggcgcgc atcgcccccg
     6061 atctcgatca ggtgctggtc gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc
     6121 cggatagccg ctcttttaaa gacaagtggc gggtacagat tcagggatgc gtggttgccg
     6181 ccgcgttatg cgccgttatc actttaattg ccatgcagtg gctgatggcg tttgatgccg
     6241 ccaacctggt gatgctgtat ctgcttggcg tggtggtagt ggcgctattt tatggacgct
     6301 ggccttcggt ggttgctacc gtcattaatg tggtgagttt cgatctcttt tttatcgccc
     6361 cacgcggcac gctcgccgtc tctgatgtgc aatatctgct gaccttcgcg gtgatgttaa
     6421 ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg ttatcaggcg cgggtagccc
     6481 gttaccgcga gcaacgcaca cggcacttat atgaaatgtc gaaagctctg gcggtgggcc
     6541 gcagtccgca ggacatcgcc gccaccagcg aacaatttat tgcctccacg tttcatgccc
     6601 gcagtcaggt gttgttgccc gatgacaacg gtaaattgca gccgttaaca catccgcaag
     6661 gaatgacgcc atgggacgat gccatcgcgc agtggagttt tgataaaggc ctgcctgcgg
     6721 gtgcgggcac cgacacgtta cccggcgtgc cgtaccagat tttgccgcta aaaagcggcg
     6781 agaaaaccta cggactggtg gtggtggaac cggggaatct gcgccagttg atgatcccgg
     6841 agcagcagcg cctgctggag acatttacgc tgttagtcgc caatgccctt gagcggctga
     6901 cactgaccgc cagcgaagaa caggcgcgga tggcaagcga acgtgaacag atccgcaacg
     6961 ccctgctggc ggcgctttcg catgatttac gcacgccgct tacggtgctg tttggtcagg
     7021 cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc ccacgcccgc caggccagcg
     7081 agattcgtca gcatgtgctg aacactaccc gactggtgaa taatctactg gatatggcgc
     7141 gaattcagtc cggcggcttt aatttgaaga aagagtggtt aacgctggaa gaagtggtcg
     7201 gtagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc catcaatctt tctctgccag
     7261 aaccgctgac cttaatccac gttgacgggc cactctttga acgggtgctg attaatctgc
     7321 tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg
     7381 agggcgaaaa tctacaactg gatgtctggg ataacggccc cggtcttccg ccaggccagg
     7441 agcagacgat atttgataag tttgctcgcg ggaataaaga gtcggcagta ccgggggtag
     7501 ggcttggact ggcaatttgt cgggcgatag tggatgtaca cgggggcact attaccgcgt
     7561 tcaaccgacc ggaaggtggt gcctgttttc gtgttacact tccccagcaa accgctcctg
     7621 aacttgaaga ctttcatgag gatatgtgac aaacgttctg attgttgaag atgaacaggc
     7681 tattcgtcgc tttctgcgca cggcgctgga gggcgacggg atgcgcgtct atgaggccga
     7741 aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag ccagatttga ttattctcga
     7801 tctcggcctg cccgatggtg atgggattga gtttatccgc gacctgcgcc agtggagcgc
     7861 ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc gacaaaatcg ccgcgctgga
     7921 tgccggagcg gatgattatc tgagtaagcc gtttggcatt ggcgaattgc aggcccgtct
     7981 gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc gatccgctgg tgaaattttc
     8041 cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct
     8101 cacgccgatt gagttccgcc tgctggcggt gctgctgaat aatgccggga aagtgctcac
     8161 ccagcgccag ttactcaatc aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt
     8221 gcgtatttat atgggacatc tgcgacaaaa actggaacag gatcccgctc gcccccgcca
     8281 tttcattact gaaaccggta ttgggtatcg gtttatgcca tgaatgttt
//
LOCUS       DAPHJE010000158         8308 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPHJE010000158 DAPHJE010000000
VERSION     DAPHJE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.160"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..105
                     /locus_tag="RH222_003994"
     CDS             <1..105
                     /locus_tag="RH222_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV3798934.1"
                     /translation="TNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGFS"
     gene            374..2611
                     /gene="nrfB"
                     /locus_tag="RH222_003995"
     CDS             374..2611
                     /gene="nrfB"
                     /locus_tag="RH222_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV3798935.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2598..5570
                     /gene="nfrA"
                     /locus_tag="RH222_003996"
     CDS             2598..5570
                     /gene="nfrA"
                     /locus_tag="RH222_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDV3798936.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5571..6461
                     /locus_tag="RH222_003997"
     CDS             5571..6461
                     /locus_tag="RH222_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV3798937.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6644..7405
                     /gene="envY"
                     /locus_tag="RH222_003998"
     CDS             6644..7405
                     /gene="envY"
                     /locus_tag="RH222_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDV3798938.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7451..7527)
                     /locus_tag="RH222_003999"
     tRNA            complement(7451..7527)
                     /locus_tag="RH222_003999"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7491..7493),aa:Arg,seq:tct)
     gene            7770..>8308
                     /gene="fimZ"
                     /locus_tag="RH222_004000"
     CDS             7770..>8308
                     /gene="fimZ"
                     /locus_tag="RH222_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV3798939.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKT"
BASE COUNT         2080 a         2143 c         2111 g         1974 t
ORIGIN      
        1 acgaatgata aggtattcaa ggcagggtta agacgtaaga tgcgaaaagc agccatggac
       61 agaaactatc tcgcgtcagt ccttgcgggg agcgggtttt cgtaatcttg ccctgcctgc
      121 tcacctcctc caggcacttc cactacatta tgacctgctc ggtgccaccg ctccttttca
      181 ctatgacgtt aacctcccct ccttgacacc atgcttttgt ctacattcta cgcaaacggc
      241 aacttttata accagtgtaa aaataacgtg ccgcaatagt catacctatt aatggtaaaa
      301 agctgtcaca attcataaaa aaccttaata tacgccaccc taaacataac cagcgttaat
      361 gtaaggtttt tgtgtggact ggcttcttga tgtttttgca acctggctct acggcttaaa
      421 agtaatcgcg ataacgttag cggtcatcat gttcatcagc gggctggacg atttttttat
      481 tgatgtcgtc tactgggtac gccgcattaa acgcaagttg agtgtttatc gccgctaccc
      541 gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa ccgttagcga ttatggttcc
      601 ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag ctggcggcga ccacgctcga
      661 ctacgaaaac tatcatatct ttgttggcac ctaccccaac gaccccgata ctcaacgtga
      721 tgttgacgaa gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg
      781 tcccaccagc aaagccgact gtctgaacaa cgtgctggac gccatcaccc agtttgaacg
      841 tagcgccaat ttcgcttttg ctggttttat tctgcatgac gccgaagatg tgatttcacc
      901 gatggaattg cgtctgttca actatctggt cgagcgtaaa gatctgattc agatcccggt
      961 gtatccgttc gaacgcgaat ggacgcactt caccagcatg acttacattg atgagttttc
     1021 agagctgcat ggcaaagatg ttccggtgcg tgaagccctc gccggacaag tgcccagcgc
     1081 aggcgtcggc acctgtttca gccgccgcgc cgtgaccgct ctgttagctg acggtgacgg
     1141 tattgctttc gacgtgcaga gtctgactga agattacgac attggtttcc gcctgaaaga
     1201 gaaaggtatg acggaaattt ttgtccgttt tccggtggtg gacgaagcca aagaacgcga
     1261 gcagcgcaaa tttttacagc acgcacggac gtcaaacatg atctgcgtgc gcgaatattt
     1321 ccccgatacc ttttcgactg cagttcgaca aaaatctcgc tggatcatcg gcattgtttt
     1381 ccagggcttt aaaacccaca aatggacctc cagcctgacg ctgaactact ttctctggcg
     1441 cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc gcgatgctgg tgatgctcca
     1501 gcttttgctg ttgctggcgt atgaaagttt gtggcccaat gcctggcatt tcctttctat
     1561 ttttagcggc agcgcatggt taatgaccct gctgtggcta aactttggct tgatggttaa
     1621 ccgcatcgtg cagcgggtga ttttcgtcac tggctactac ggtctgacgc aggggctgct
     1681 atctgtcctg cgtcttttct ggggcaacct gattaacttc atggccaact ggcgcgcgct
     1741 aaaacaggta cttcaacacg gcgatccacg tcgcgtcgcg tgggataaaa caacgcatga
     1801 cttccccagc gtgactggcg atacccgctc gttgcgcccg ttaggccaaa ttctgctgga
     1861 aaatcaggtc atcactgaag aacaactcga tacagcactg cgtaatcgcg tcgaaggtct
     1921 acgcctgggc ggttcaatgc tgatgcaggg gctgattagc gccgagcagc tggcacaggc
     1981 gctggcagag caaaacggcg tggcgtggga atccatcgat gcctggcaga tcccgtcctc
     2041 gctgattgcc gaaatgccag cctccgtagc gctgcattat gcagtactgc cgctgcgtct
     2101 ggaaaatgat gagttaattg tcggcagtga agatggtatt gacccggttt cgctggcggc
     2161 cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt
     2221 cacggggtta cgtcactggt atgcacgccg acgcggtcac gatccgcggg caatgttgta
     2281 caatgcggtt cagcatcagt ggctcacgga acagcagacc ggtgaaatct ggcggcaata
     2341 tgtgccgcat cagttcctgt tcgccgaaat actgaccacg ctcggtcata ttaatcgttc
     2401 agcaattaac gtgttgttat tgcgccatga acgcagttct ctgccgctcg gcaagttttt
     2461 ggtcaccgaa ggcgttatca gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga
     2521 attacaagtt tcgatgcaat cactattact caaagcaggt ttaaacacag aacaggttgc
     2581 gcaactggag tccgaaaatg aaggagaata accttaatcg cgtcatcgga tggtctggtt
     2641 tactgctgac gtctttattg agtaccagcg cactcgcaga caatatcggc accagcgcag
     2701 aagagctggg gctgagcgat tatcgccatt ttgttattta tccccgtctc gataaggcgc
     2761 tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa tatatacacc
     2821 agcaggtgcc ggataatatt ccgctgactt tataccttgc ggaagcctat cgccattttg
     2881 gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac ccaggagatg
     2941 cccgacttga gcgcagtctg gcggctattc cggttgaagt gaaaagcgtt acgactgttg
     3001 aagaactgct tgcccagcaa aaagcgtgcg acgctgcgcc gaccctgcgt tgtcgcagtg
     3061 aagtcgggca gaatgccctg cggctggcac aattacctgt cgccagagcg caactgaacg
     3121 atgcgacgtt tgctgcatcg ccagaaggaa aaacgctgcg aaccgatctg ctgcaacggg
     3181 caatctacct gaaacaatgg tcccaggcag atacgctata caatgaagca cgccagcaga
     3241 acacattaag cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg
     3301 acgatcggat cctggcactg caatcacagg ggatcttcac cgatcctcag tcatatatta
     3361 cttacgcgac cgcgctggct tatcgtggcg aaaaagcacg cctccagcat tatctcaatg
     3421 aaaataagcc gctgtttacc acggacgcac aagagaaaag ttggctctat ctgttatcta
     3481 aatacagcgc caaccccgtt caggcattgg cgaattatac ggtgcagttt gccgataacc
     3541 gccagtatgt tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag tacgacgcag
     3601 cgcaaaaact gctcgccacc ctccccgcca atgaaatgct tgaggagcgt tatgctgtca
     3661 gcgtggcgac ccgtaacaag gctgaatctc tgcgtctggc acgattgctg tatcagcaag
     3721 aaccggcaaa tcttacccgc ctggatcaac tgacctggca actgatgcag aacgagcagt
     3781 cacgcgaagc tgccgattta ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca
     3841 gccagacttt aatggcgcga ctggcgtctc tgctggaaag tcatccttac ctggcaacgc
     3901 cggcgaaggt ggcgatttta tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa
     3961 gtcagttgcc gggtattgca gataattgcc cggcaatagt tcgcttgctg ggcgatatgt
     4021 cgccttccta cgatgctgcc gcctggaacc gtctggcaaa gtgttatcgg gacacgctac
     4081 ccggtgtggc gctgtatgca tggcttcagg ccgaacaacg acaaccgaac gcctggcaac
     4141 atcgtgcggt agcctatcag gcgtatcagg ttgaggacta cgccaccgca ctggcggcct
     4201 ggcagaaaat cagtcttcac gacatgagca atgaggatct gcttgctgct gccaataccg
     4261 cccaggcggc aggaaatggt gcagctcgcg atcgctggct acaacaggca gaacaacgtg
     4321 gactgggaaa caatgccctc tactggtggc tgcatgcgca acgttacatt cctggtcagc
     4381 cggaactcgc actgagcgat ctcacgcgct caatcaatat tgcgccttct gccaacgctt
     4441 acgttgcgcg ggcgacaatt tatcgccaac gtcataatgt cccggcggcg gtgagtgatt
     4501 tgcgcgccgc gctggaactg gaaccgaata atagcaacat ccaggcagcg cttggttacg
     4561 ccttgtggga tagcggtgat atcgcacagt cgcgggaaat gctcgaacag gcgcataaag
     4621 ggcttccgga cgatccggca ctgatccgac aactggccta tgtgaaccag cgtctggatg
     4681 acatgcctgc gacgcagcat tacgcccggc tggtgattga tgacattgat aatcaggcgc
     4741 tgataacccc actgacccca gagcaaaatc agcaacgctt caatttccgc cgtttgcatg
     4801 aggaggtcgg tcgccgctgg acgttcagtt tcgattcttc tatcggcttg cgttccgggg
     4861 caatgagcac cgctaacaat aatgtcggcg gcgcagcgcc agggaaaagc tatcgtagct
     4921 acgggcaact ggaagccgag taccgcatcg gacgcaatat gctgctggaa ggcgacctgc
     4981 tctcagttta tagccgcgtc tttgccgata ccggagaaaa cggggtgatg atgccggtga
     5041 aaaatccgat gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag atctttttcc
     5101 tcgccgtcga acagcagttg ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc
     5161 gcgccagcgc ctcattcttt aatggcggca aatacagcga cgaatggcac ccgaacggtt
     5221 caggctggtt tgcccaaaac ctgtacctcg atgcggcgca atatatccgc caggatattc
     5281 aggcgtggac ggcagattat cgcgtcagct ggcatcagaa ggtagctaac ggacagacta
     5341 ttgagcctta cgctcacgtt caggacaacg gctatcgtga taaaggcact cagggcgcgc
     5401 agcttggcgg agtcggggtc cgctggaata tctggaccgg cgagacgcac tacgacgcct
     5461 ggccgcacaa agtcagtctc ggcgtcgagt atcaacatac ctttaaggcg attaatcaac
     5521 gtaacggaga gcgcaacaac gcgtttctca ccattggagt gcactggtaa atgcgtaagt
     5581 tcattttcgt attgctgaca ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta
     5641 ttatctggca accacaaaac cgagatagtc aggttaccga tacccagtgg caggggctga
     5701 tgagtcagtt acgtttgcaa ggcttcgata ccctggtttt gcaatggacc cgttacggcg
     5761 atgcattcac ccagccagaa cagcgcgcgt tattgtttaa gcgtgccgca gctgcgcaac
     5821 aggctggcct gaagctgatt gtcgggctga acgccgatcc ggaatttttt atgcaccaga
     5881 aacagtcgtc cgcagcgctg gaaagctatc ttaatcgcct gctggctgcc gatctccagc
     5941 aagccagatt atggagcgcc gcgcctggcg taacgccgga tggctggtac atcagcgcgg
     6001 aaattgacga cctgaactgg cgcagcgaag ctgcccgtca gcctttgcta acatggttaa
     6061 acaacgcgca gcggctgatt agcgatgttt cagcaaaacc ggtttatatc agtagttttt
     6121 tcgccggaaa catgtcgccc gatggctatc gccaactgct ggaacaagtt aaagcaaccg
     6181 gcgttaatgt ctgggtacag gatggcagcg gcgtggataa acttaccgct gaacagcgtg
     6241 aacgttatct acaggccagc gccgattgcc aaagtcccgc ccctgccagc ggcattgttt
     6301 atgaactgtt tgtcgccggc aaaggcaaaa cctttacagc gaaaccgaaa ccggatgcag
     6361 agatcgcctc gctgttagcg aaacgttcct cttgcggtaa agacactctc tatttctctc
     6421 tgcgctattt gcccgtcgcg cacggcattc tcgagtatta aatctcctcc aggtaagtcg
     6481 ggtacgacct ggcttacctc tttcgctctc gtcagaattc catcaagatt aattgctaaa
     6541 aagcgtgcta aagaaagaag atcgttatcg gcgaacgggc cgatcactca tgcaggaagc
     6601 atagatcctt tgagcggagt ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag
     6661 tgaaccttgc gtggtgatcc tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc
     6721 tacgtttacc cttccgaaaa actacctggc cgccttcgcg tgcaacaata acgtcattga
     6781 actctcaacg ttaaatcacg tattaatccc ccacatcaac cgtaacatca tcaacgatta
     6841 tctgttgttt ttaaataaaa acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
     6901 ggttatcgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
     6961 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt
     7021 gtctaacttt cttgagcaat cgcggtttat agccctactg atgtatatct tacgcagcag
     7081 cgtccgcgac agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
     7141 aattgtcgcc agttcgctat gtttaagccc cagcctactc aaaaagaaat taaaaaacga
     7201 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
     7261 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
     7321 gtactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcgccaa
     7381 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
     7441 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt
     7501 tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat
     7561 tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga
     7621 ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacacaca ccgctgcgcg
     7681 tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa
     7741 aattgtccgc tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata
     7801 ctcatcctat catcagaatg tctattgaag ttctgttgca aaaaaacagt gaattgcaga
     7861 ttgtcctgaa aacggatgat taccgcataa ccatcgatta tctccgaacc cgtcctgttg
     7921 atttaatcat tatggatata gacttgcccg gaacagacgg ttttaccttc ctgaaaagga
     7981 tcaaacaaat ccagagcaca gtgaaagtgt tatttttatc atcgaaatca gaatgctttt
     8041 atgctggcag agcgatacaa gctggtgcta acggttttgt cagtaaatgc aatgatcaga
     8101 atgatatttt tcatgccgtt cagatgatcc tctccggata cacgtttttt cccagcaaaa
     8161 cgcttaacta tataaaaagc aataaatgta gtacgaatag ttcaacgatc actgtgctat
     8221 ctaatcgtga agtgaccata ttacgttatc tggttagcgg attatctaat aaagaaattg
     8281 ccgataagtt attacttagc aataaaac
//
LOCUS       DAPHJE010000159         8137 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPHJE010000159 DAPHJE010000000
VERSION     DAPHJE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.161"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            167..1270
                     /gene="proB"
                     /locus_tag="RH222_004001"
     CDS             167..1270
                     /gene="proB"
                     /locus_tag="RH222_004001"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV3798940.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1282..2535
                     /gene="proA"
                     /locus_tag="RH222_004002"
     CDS             1282..2535
                     /gene="proA"
                     /locus_tag="RH222_004002"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV3798941.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2650..2725
                     /locus_tag="RH222_004003"
     tRNA            2650..2725
                     /locus_tag="RH222_004003"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2683..2685,aa:Thr,seq:cgt)
     gene            complement(2740..3903)
                     /locus_tag="RH222_004004"
     CDS             complement(2740..3903)
                     /locus_tag="RH222_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3798942.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRNEENWKLRAEACRKKGKPVPEYKPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(4102..4380)
                     /locus_tag="RH222_004005"
     CDS             complement(4102..4380)
                     /locus_tag="RH222_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV3798943.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            complement(4428..4646)
                     /locus_tag="RH222_004006"
     CDS             complement(4428..4646)
                     /locus_tag="RH222_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDV3798944.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQVISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            complement(4745..4960)
                     /locus_tag="RH222_004007"
     CDS             complement(4745..4960)
                     /locus_tag="RH222_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV3798945.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(5037..5228)
                     /locus_tag="RH222_004008"
     CDS             complement(5037..5228)
                     /locus_tag="RH222_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDV3798946.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(5201..5383)
                     /locus_tag="RH222_004009"
     CDS             complement(5201..5383)
                     /locus_tag="RH222_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HDV3798947.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVTRNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            complement(5380..6060)
                     /locus_tag="RH222_004010"
     CDS             complement(5380..6060)
                     /locus_tag="RH222_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDV3798948.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVTPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYHDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(6057..6842)
                     /gene="bet"
                     /locus_tag="RH222_004011"
     CDS             complement(6057..6842)
                     /gene="bet"
                     /locus_tag="RH222_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDV3798949.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(6848..7144)
                     /locus_tag="RH222_004012"
     CDS             complement(6848..7144)
                     /locus_tag="RH222_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HDV3798950.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            complement(7220..7510)
                     /locus_tag="RH222_004013"
     CDS             complement(7220..7510)
                     /locus_tag="RH222_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798951.1"
                     /translation="MTTGGHPPPEYAQKSLYVQQPSLRAGVFMVHQHYGTQTVNRGAV
                     MPGMLVKRKDGTWTASANLRGRLYLHRGIERTYTRDLLVEVFLDGRGNGLNH"
BASE COUNT         1883 a         1979 c         2072 g         2203 t
ORIGIN      
        1 ggtaatttat taaatctgta ataaaagcgt aaacaactgc cgctaggctt gctgatcccg
       61 cgcaacaaaa cgccatgctt tgcttgcaga tggttggcaa ccgacgacag tcctgctaaa
      121 acgttcgttt gatatcattt ttcctaaaat tgaatggcag agaatcatga gtgacagcca
      181 gacgctggtg gtaaaactcg gcaccagtgt gctaacaggc ggatcgcgcc gtctgaaccg
      241 tgcccatatc gttgaacttg ttcgccagtg cgcgcagtta catgccgccg ggcatcggat
      301 tgttattgtg acgtcgggcg cgatcgccgc cggacgtgag cacctggggt acccggaact
      361 gccagcgact atcgcctcga aacaactgct ggcggcggta gggcagagtc gactgattca
      421 actgtgggaa cagctgtttt cgatttatgg cattcacgtc gggcagatgc tgctgacccg
      481 tgctgatatg gaagaccgtg aacgcttcct gaacgcccgc gacaccctgc gtgcgttgct
      541 cgataacaat atcgttccgg taatcaatga gaacgatgct gtcgctacgg cagagattaa
      601 ggtcggcgat aacgataacc tttctgcgct ggcggcgatt ctggcgggtg ccgataaact
      661 gttgctgctg accgatcaaa aaggtttgta taccgctgat ccgcgcagca atccgcaggc
      721 agaactgatt aaagatgttt acggcattga tgacgcactg cgcgcgattg ccggtgacag
      781 cgtttcaggc ctcggaactg gcggcatgag taccaaattg caggccgctg acgtggcttg
      841 ccgtgcgggt atcgacacca ttattgccgc gggcagcaag ccgggcgtta ttggtgatgt
      901 gatggaaggc atttccgtcg gtacgctgtt ccatgcccag gcgactccgc ttgaaaaccg
      961 taaacgctgg attttcggtg cgccgccggc gggtgaaatc acggtagatg aaggggcaac
     1021 tgccgccatt cttgaacgcg gcagctccct gttgccgaaa ggcattaaaa gcgtgactgg
     1081 caatttctcg cgtggtgaag tcatccgcat ttgcaacctc gaaggtcgcg atatcgccca
     1141 cggcgtcagt cgttacaaca gcgatgcatt acgccgtatt gccgggcacc actcgcaaga
     1201 aattgatgca atactgggat atgaatacgg cccggttgcc gttcaccgtg atgacatgat
     1261 tacccgttaa ggagcaggct gatgctggaa caaatgggca ttgccgcgaa gcaagcctcg
     1321 tataaattag cgcaactctc cagccgcgaa aaaaatcgcg tgctggaaaa aatcgccgat
     1381 gaactggaag cacaaagcga aatcatcctc aacgctaacg cccaggatgt tgctgacgcg
     1441 cgtgccaatg gccttggcga agcgatgctt gaccgtctgg cactgacgcc cgcacggctg
     1501 aaaggcattg ccgatgatgt gcgccaggtg tgtaacctcg ccgatccggt ggggcaggta
     1561 atcgatggca gcgtactgga cagcggcctg cgtcttgagc gtcgtcgcgt accgctgggg
     1621 gttattggcg tgatttatga agcgcgcccg aacgtgacgg ttgatgtcgc ttcgctgtgc
     1681 ctgaaaaccg gtaacgcagt gattctgcgt ggcggtaaag aaacctgtcg cactaacgcg
     1741 gcaacggtgg cggtgattca ggacgccctg aaatcctgtg gcttaccggt gggtgccgtg
     1801 caggcgattg ataatcctga ccgtgcgctg gtcagtgaaa tgctgcgtat ggataaatac
     1861 atcgacatgc tgatcccgcg tggtggcgct ggtttgcata aactgtgccg cgaacagtcg
     1921 acgatcccgg tgatcacagg tggtataggc gtatgccata tttatgttga tgaaagtgta
     1981 gagattgctg aagcattaaa agtgatcgtc aacgcgaaaa ctcagcgtcc gagcacatgt
     2041 aatacggtag aaacgttgct ggtgaataaa aacatcgccg atagcttcct gcccgcatta
     2101 agcaaacaaa tggaggaaag cggcgtggca ttacacgcag atgcagctgc gctggcgcag
     2161 ttgcagacag gccccgcgaa ggtggtggct gttaaagcgg aagagtatga cgatgagttt
     2221 ctgtcattag atttgaacgt caaaatcgtc agtgatcttg acgatgccat cgcccatatt
     2281 cgtgaacacg gcacacaaca ctccgatgcg atcctgaccc gcgatatgcg caacgcccag
     2341 cgttttgtta acgaagtgga ttcgtccgct gtttacgtta acgcctctac gcgttttacc
     2401 gacggcggcc agtttggact aggtgcggaa gtggcggtaa gcacacaaaa actccacgcg
     2461 cgtggcccaa tggggctgga agcactgacc acttacaagt ggatcggtat tggtgattac
     2521 accattcgtg cgtaaataaa accgggtgat gcaaaagtag ccatttgatt cacaaggcca
     2581 ttgacgcatc gcccggttag ttttaacctt gtccaccgtg attcacgttc gtgaacatgt
     2641 cctttcaggg ccgatatagc tcagttggta gagcagcgca ttcgtaatgc gaaggtcgta
     2701 ggttcgactc ctattatcgg caccattaaa atcaataagt tacacatcat tagtaccttc
     2761 tttatttttt gactgggaca aatttgggac cgatgggttc aggatcgagt ctatttgccg
     2821 tgcgtgttcg gtaaggtgat taggtgcaag gtgagcatat cgacgaacca tttcgataga
     2881 ctcccagcct cccatttcct gtaacactga caatgggact ccggcttgaa ccagccaact
     2941 tgcccaggtg tgtctcaagt cgtgaaatct gaaatcatca ataccagccc gtctcagcgc
     3001 cgctttccag gctgtgtttg cgtcataccg catcttcctt actgttggcg ctttcgttcc
     3061 gtctggtttg gtacagcttt ccttgtacac aaatacccaa cggtgatgat tcccgatttg
     3121 ttttttcaat acgcgacatg cagtatcatt cagcgcaacg ccaattgcgc ggtttgattt
     3181 actctcttcc gggtttatcc atgccacccg gcgctgcatg tctatttgtt gccattcaag
     3241 gttgatgatg ttcgagcgtc ttaagcctgt tgccagtgca aattcaacaa cagactttaa
     3301 tggctccgga cattcatcaa tcagcctttg tgcttcatgg ggctccagcc agcggatccg
     3361 tttattcttt ggttgaggca ctttaataat tggtgcctta tccagcattt tccattcacg
     3421 ctctgcggct cttagtaggg cttttataaa tgaaagatgc gtagccttcg ttgcaacaga
     3481 cgctggtttt ggcttgtatt ctggaacagg tttccctttt tttctgcatg cttctgccct
     3541 gagtttccag ttttcctcat tacgccggtt cgtcattttc tgcattgctg aataaatttt
     3601 tgattcagta atgtctctta gttgcattcc tgcgaaatgt tgaagccaga atccgatccg
     3661 gcttttgtca tcgtccagtg attttttatg tgctttctct tcaagccacc tgacacacgc
     3721 ttcctcgaac gttatatcag gtatttcacc aagtttgctg acccgccatg cttcagcctt
     3781 tagcttgtca tggagttctg tcgcctgcct tttgtccttt gttccaagag actgtttaaa
     3841 tcttttaccg ttcggcaatg tgaaactggc gtaccatatt tcacctctgc ggaagagtga
     3901 cattttcttt cctctgttat gccatcaccc gcgctcacct ggacagtatg cagcggagac
     3961 tgaagcgccg caatgcaggc ttgtcgtgtt gtgaggtaag gagattttag tttagtgggg
     4021 tctttgcgtg ttgcctgtag gcggcctgtt cgtatccagt tggtagcggt aggtctggat
     4081 atcttgagaa actgacaggc ctcatcgagt gtgaggctgt atgattccat ggttacctct
     4141 gctttttgaa cgcatgtcac gtaacttctt aatgtgttct gccgtttcga tctcttctgc
     4201 tatccgatct gcatcagctt tattcacagg ttcaaagtca tgattaaagc ggaacatgct
     4261 ggcgatacat gttctgcctt ttcggatgta gtgaactttg ttgtgggtag aacgcaggat
     4321 tttgcaggga gtgccgtggt ggtcgacgta ccaggtgtta ggaaaaatga ttctgaacat
     4381 ttttacacct cagttggacg atgttgaaat ttgctgcttt gaggccatca caatccccat
     4441 tgtttgttct taagttcgat ctcctcctgg caacttgcac aagtccgaca accctgaacg
     4501 gccaggcgtc ttcgttcatc tatgggatcg ccacactcac aacaatgagt ggcagatata
     4561 acctggtggt tcagacgacg catttttatt gctgtattgc gctgtaattc ttcgatttct
     4621 gatgctgaat caatgatgtc tgccatcttc cattaatccc tgaattgttg gttaatacgc
     4681 ttgagggtga atgcgaataa taaaaaagga gcctgtagct ccctgatgat tttgcttttc
     4741 atgttcatcg ctccttaaag acgccgttta acatgccgat cgccaggctt aaatgagtcg
     4801 gtgtgaatcc catcagcgtt accgtttcgc ggtgcttctt cagtacgcta cggcaaatgt
     4861 catcgacgtt tttatccgga aactgctgtc tggctttttt gatttcagaa ttagcctgac
     4921 gggcaatact gcgaagggcg ttttcttgct gaggtgtcat tgaacaagtc ccatgtcggc
     4981 aagcataagc acacagaata tgaagcccgc tgccagaaaa atgcattcag tggttgtcat
     5041 acctggtctc tctcatctgc ttctgctttc gccaccatca tttccagctt ttgtgaaagg
     5101 gatgcggcta acgtatgaaa ttcttcgtct gtttctactg gtattggcac aaacctgact
     5161 ccaatttgag cgaggctatg tgccatctca atgctcgttc ttaactcaac aggagatgct
     5221 ttgtgcatac agctccccgt ttattattta tctcctcagc cagccgctgt gctttcaggg
     5281 gatttcgggt aacagaaagg ccgggaaata cccagcctcg ctttgtaacg gagtagacga
     5341 aagtgatcgc gcctacccgg atattatcgt gaggatgcgt catcgccatt gctccccaaa
     5401 tacaaaacca atttcagcca gtgcctcgtc cattttttcg atgaactccg gcaccatctc
     5461 gtcaaaactc gccatgtact tttcattccg ctcaatcacg acataatgca ggccttcacg
     5521 cttcatgcgc gggtcatagt tggcaaagta ccaggcatct tttcgcgtca cccacatgct
     5581 gtactgcacc tgggccatgt aagccgattt tattgcctcg aaaccaccga gccggaactt
     5641 catgaaatcc cgggaggtaa acgggcattt cagttcaagg ccgttgccgt cactgcataa
     5701 accatcggga gagcaggcgg tgcgcatact ttcgtcgtga tagatgatcg gggattcagt
     5761 aacattcacg ccggaagtga attcaaacag ggttctggcg tcgttctcgt actgttttcc
     5821 ccaggccagc gccttagcat taacttccgg agtcacaccg gtgcaaacct cagccagcag
     5881 ggtgtggaag taggacattt tcatgtcagg ccacttcttt cctgagcggg gctttgctat
     5941 cacgttgtga acttctgaag cggtgatgac gccgagccgt aatttgtgcc atgcatcatc
     6001 cccctgttcg acagctctca cgtcgatccc ggtacgctgc aggataatgt ccggtgtcat
     6061 gctgccacct tctgctcagt ggctttctgt ttcaggaatc caagagcttt cactgcttcg
     6121 gcctgtgtca gttctgacga tgcgcgaatg tcgcggcgaa atatctggga acagagcggc
     6181 aataagtcgt catcccatgt tttatccagg gctatcagca gagtgttaat ctcctgcatg
     6241 gtttcatcgt taaccggagt gatgtcgcgt tctggctgac gttctgcagt gtatgcagta
     6301 ttttcgacaa tgcgctcggc ttcatccttg tcatagatac cagcaaatcc gaaggccaga
     6361 cgggcacact gaatcatggc tttatgccgt aacatccgtt tgggatgcga ctgccacggc
     6421 cccgtgattt ctctgccttc gcgagttttg aatggttcgc ggcggcattc atccatccat
     6481 tcggtaacgc agatcggatg attacggtcc ttgcggtaaa tccggcatgt acaggattca
     6541 ttgtcctgct caaagtccat gccatcaaac tgctggtttt cattgatgat gcgggaccag
     6601 ccatcaacgc ccaccaccgg aacgatgccg ttctgcttat cagggaaggc gtaaatttct
     6661 ttcgtccacg gattaaggcc gtactggttg gcgacgatca acaatgcgat gaactgcgca
     6721 tcgctggcat cacctttaaa tgccgtctgg cgaagagtgg tgatcagttc ctgtgggtcg
     6781 acagaatcca tgccgacacg ttcagccagc ttccctgcca gcgttgcgag tgctgtactc
     6841 atccgtttta tacctctgaa tcaatatcaa cctggtggtg agcaatggtt tcaaccatgt
     6901 actggatgtg ttctgccatg cgctcctgaa actcaacatc gtcatcaaac gcacgggtaa
     6961 tggctttttt gctggccccg tggcgttgca aatgatcgat gcatagcgat tcaaacaggt
     7021 gctggggcag gcctttttcc atgtcgtctg ccagttctgc ctctttctct tcacgggcga
     7081 tctgctggta gtgacgcgcc cagctctgag cctcaagacg atcctgaatg taataagcgt
     7141 tcatggctga actcctgaaa atggctgtga aaaaatcgcc cgcggaatgc caggctgatt
     7201 cggaaaacag gaaaggggat tagtgattca gaccgttgcc gcgtccgtcg agaaaaactt
     7261 ccacgagcaa gtcacgggta taagtgcgct caatgccgcg atgcagataa agtcgtccgc
     7321 gtaaattagc tgatgcagtc caggtaccat ctttgcgttt gaccagcatt cctggcatga
     7381 ccgcaccgcg attaacggtc tgcgttccgt aatgttgatg aaccataaaa actcctgccc
     7441 gtaagctggg ctgctgaaca tataaagact tctgcgcgta ttcaggcggt ggatggccgc
     7501 cggttgtcat aactaagccg cctcgttgaa gcgactaagg tatgaaatgt tgagttgatt
     7561 tcagctggtc acaccgacgt tcacgcgtcc gtttcatccc tcgcactccc cgaagcctgc
     7621 tgaaattcaa gctgcggatc taagcggtca tcgcaacggt gaatcaggtg gttgccgtat
     7681 cgttgtgttg ttgcgatatg gtgataatag ctattgctat tggtgatatc aatacttatt
     7741 gctattagtt gatgtgtttt gatattaaat gtttgatagc aaaaagaatt aattttgtga
     7801 cttgcatcgc atagcgataa ctgaagtgag gtcgtggtgg ttttttgaac ggtgtgtgat
     7861 gaggggaggg caaaagaaaa cccggcacgg tggccgggtt attgttcttg attgatctta
     7921 tcgaagatat catgacttaa tttttgcaca tttaggaaat ttactatgaa gaagacaatt
     7981 gcgtaaatta ttaatgtgaa aaaaagtatt gaaggagaga attttagtat cagggattta
     8041 aattcaaagc tgaactcata ctcttttaaa tattcactaa gcacaaggca taaagtagaa
     8101 agagtgaaaa agcgcaaaaa agaatttcgt atagata
//
LOCUS       DAPHJE010000160         8014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPHJE010000160 DAPHJE010000000
VERSION     DAPHJE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.162"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..550
                     /locus_tag="RH222_004014"
     CDS             32..550
                     /locus_tag="RH222_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV3798952.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(595..2640)
                     /gene="dcp"
                     /locus_tag="RH222_004015"
     CDS             complement(595..2640)
                     /gene="dcp"
                     /locus_tag="RH222_004015"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV3798953.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2777..3523
                     /gene="ydfG"
                     /locus_tag="RH222_004016"
     CDS             2777..3523
                     /gene="ydfG"
                     /locus_tag="RH222_004016"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV3798954.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3612..4298
                     /gene="ydfH"
                     /locus_tag="RH222_004017"
     CDS             3612..4298
                     /gene="ydfH"
                     /locus_tag="RH222_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV3798955.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4476..4679
                     /gene="ydfZ"
                     /locus_tag="RH222_004018"
     CDS             4476..4679
                     /gene="ydfZ"
                     /locus_tag="RH222_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV3798956.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4715..6175)
                     /locus_tag="RH222_004019"
     CDS             complement(4715..6175)
                     /locus_tag="RH222_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV3798957.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPTVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6264..7547)
                     /gene="ydfJ"
                     /locus_tag="RH222_004020"
                     /pseudo
     CDS             complement(6264..7547)
                     /gene="ydfJ"
                     /locus_tag="RH222_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         2111 a         2044 c         1839 g         2020 t
ORIGIN      
        1 cctttacata taatagcgtg gacaaaaaac agtgaacatt aataaagata aaattgtaca
       61 actcgcagat accgacacta ttgaaaacct gacatccgcg ttgagtcaaa gacttatcgc
      121 ggatcaatta cgcttaacta ccgccgaatc atgcaccggc ggtaagttgg ctagcgccct
      181 gtgtgcagct gaagatacac ccaaatttta cggtgcaggc tttgttactt tcaccgatca
      241 ggcaaagatg aaaatcctca gcgtaagcca gcaatctctt gaacgatatt ctgcggtgag
      301 tgagaaagtg gcagcagaaa tggcaacagg tgccatagag cgtgcggatg ctgatgtcag
      361 tattgccatt accggctacg gcggaccgga gggcggtgaa gatggtacgc cagcgggtac
      421 cgtctggttc gcgtggcata ttaaaggcca gacctacact gcggttatgc attttgctgg
      481 cgactgcgaa acggtattag ctttagcggt gaggtttgcc ctcgtccagc tgctgcaatt
      541 actgctataa ccaggctggc ctggcgatat ctccaggcca gccattggtg gtgtttatat
      601 gttcaagcca cgatgttgca gcatcggcat aatctgagga gccttaccgc gccattgtcg
      661 atacagccgt tccagatctt cgctgttacc tctggaaagg atcgcctcgc gaaaacgctg
      721 cccattttca cgcgttaatc cgccctgctc aacaaaccac tggtaaccat catcggccaa
      781 catttgcgtc cacagataag cgtaataacc tgcagcatat ccgccaccaa aaatatgggc
      841 gaaataactg ctgcgatagc gtggcggtat agcaggaaga tccatatttt ccgccaccag
      901 cgcccgcaat tcaaaatcat cgacatcctg cattgcttcg ttttcttcca ggcaatgcca
      961 gcgcatatcg agaagtgcgg cgctaagcag ttcgctcatc tcataccctt tgttgaacag
     1021 gctggcatta cgcattttct gttgcagttc gtcaggcatt gctgccccgc tctgataatg
     1081 ccgggcgtag cgagcgaata cctgcggatg cgttgcccag tgttcgttga tttgcgacgg
     1141 aaattcgaca aaatcacgcg gcgtgttggt gccggaaagc gtggcataac gctggcgggc
     1201 aaaaaggccg tgcaacgtat gaccaaattc atggaataag gttatgacat catcccagag
     1261 taacaacgca ggctcaccgg cagcgggttt ctgataattg caaacgttat aaattaccgg
     1321 atgcgtttca ttaagcgttg attgctcaac aaaattgccc atccatgcac cgccgctttt
     1381 tgaatcacgg gcgaagaaat caccgtaaaa taacgccagc cccacgccat tatgatcaaa
     1441 aatttcccac acccgaacgt caggatggta gacaggaata tcaaaacgtt cgacaaactt
     1501 aataccgaag agctgattcg cggtccagaa tacaccttca ttcaacaccg tgtttaattc
     1561 aaaatatggc ttgagctgcg cctcatcaag atcaaatttc tcccgccgta cctgttccgc
     1621 ataaaatgcc cagtcccacg gctgcgcgct aaacccgccc tgctgcttat cgataaccgc
     1681 ctgtatggag gctaattcat cgctcgcacg ttgacgcgcc gctggaacaa tttcccgcat
     1741 aaagttgagt gctgcttctg gcgtttttgc catctgatcg gcgattttcc atgcggcata
     1801 atgaggaaaa ccaagcagtt tcgcctgctg tgcgcgaatc tctaccagac gttgaatgat
     1861 agcgcgggta tcattgccat catttttttc cgctcgcgtc cagcccgcaa taaacagttt
     1921 ttcacgcgtc gcacgatcgc gcatttcggc aagcgccggt tgttgggtgg tattcagcag
     1981 cggaatcagc catttgttat ccagaccttt ctcgcgagcc gccactgccg ccagcgcaat
     2041 ctcttgctca ctcattcctg ccaactgcgc gatatcgttc acaaccagac cgccggattt
     2101 atttgctgca agtaaccgct ggttaaactg gctggtcagg gtcgcagctt ctgtattcag
     2161 tacttttaat tttgctttat cagcttgcgc aagtttggct ccggcaagga caaaacgttg
     2221 atgaataacc tccaccaggc ggatggattc actatcaagc cccagggatt cacggcgctg
     2281 ccagacagca tctacccgcg cgaataattc accgttcaga tagatatcat tagccagttc
     2341 cgccagttca gcggaaaact gttcatcaag acgctgtaat tcatcattgg tatgcgccgc
     2401 agtcatcgca aaaaagacgc tggtaacgcg ggtaagtaat tctccgcttt gttccagtgc
     2461 cagaatagta ttgttgaaat caggcgtttg cgggttaagc gcgatggcag caatttctgc
     2521 ccgcttttgc tgcattccct catcgaatgc cgggcgatag tgatgattgg caatttgatc
     2581 aaaatgagga gccagatacg gcagtgtgct ttgcacaagg aaaggattca ttgttgtcat
     2641 tttcttctcc tgaacgcgag gtgttccata gcgtaggctt actgataacg gagtgcaatc
     2701 ttgcaaacca gtattacccg ctcttaagca tcacgtgcta tgttattgac acacaaaagc
     2761 gttgaggaac agtgagatga tcgttttagt aactggagca acggcaggtt ttggtgaatg
     2821 cattactcgt cgttttattc aacaagggca taaagttatc gccactggcc gtcgccagga
     2881 gcggttgcag gagttaaaag acgaactggg agataatctg tatatcgccc aactggacgt
     2941 tcgcaaccgc gccgctattg aagagatgct ggcatcgctt cctgccgagt ggtgcaatat
     3001 tgatatcctg gtaaataatg ccggcctggc gttgggcatg gagcctgcgc ataaagccag
     3061 cgttgaagac tgggaaacga tgattgatac caacaacaaa ggcctggtat atatgacgcg
     3121 cgccgtctta ccgggtatgg ttgaacgtaa tcatggtcat attattaaca ttggctcaac
     3181 ggcaggtagc tggccgtatg ccggtggtaa cgtttacggt gcgacgaaag cgtttgttcg
     3241 tcagtttagc ctgaatctgc gtacggatct gcatggtacg gcggtgcgcg tcaccgacat
     3301 cgaaccgggt ctggtgggtg gcaccgagtt ttccaatgtc cgctttaaag gcgatgacgg
     3361 taaagcggaa aaaacctatc aaaataccgt tgcattgacg ccagaagatg tcagcgaagc
     3421 cgtctggtgg gtgtcaacgc tgcctgctca cgtcaatatc aataccctgg aaatgatgcc
     3481 ggttacccaa agctatgccg gactgaatgt ccaccgtcag taatttttat acccggcgta
     3541 actgccgggt tattgcttgt cacaaaaaag tggtagactc atgcagttaa ctcactcaca
     3601 agcaagaacg aatgaccgtc gaaacgcaac ttaatcccac acagcctgtc aatcagcaga
     3661 tttatcgtat tcttcgtcgc gacattgtcc attgcctgat tgcaccaggc acaccgttgt
     3721 cggaaaaaga agtttctgtt cgtttcaatg tgtcacgcca gccggttcgt gaagccttta
     3781 ttaaactggc ggaaaacggc ctgattcaaa ttcgtccgca gcgtggcagc tacgtcaaca
     3841 aaatttccat ggcccaggtg cgaaacggca gttttatccg tcaggccatt gagtgcgcgg
     3901 tggcgcgtcg ggcggcgagc atgattaccg aaagccagtg ctaccaactg gaacaaaatc
     3961 ttcaccagca acgcattgcc attgagcgca agcaactgga tgattttttt gaacttgatg
     4021 acaacttcca tcaactcctg acgcagattg ccgactgtca actggcgtgg gataccattg
     4081 agaacctgaa agcgaccgtt gatcgcgtgc gctatatgag tttcgaccat gtttctccac
     4141 cagaaatgct gttacgccag catcttgata ttttctctgc cctgcaaaaa cgtgatggcg
     4201 atgcggtaga acgtgcaatg acgcaacatt tgcaggaaat cagcgaatcc gtgcgccaga
     4261 tccgccagga aaacagcgac tggtttagcg aagagtaatt catttactct catcccatcc
     4321 gggatgagag gcttttcccc ccgccttacg gctcatgcat gcatcaaaaa agatgtgagc
     4381 ttgatcaaaa acaaaaaata tttcactcga caggagtatt tatattgcgc ccgttacgtg
     4441 ggcttcgact gtaaatcaga aaggagaaaa cacctatgac gacctacgat cgtaaccgta
     4501 acgcaatcac cactggcagc cgtgttatgg ttagcggcac cggtcacact ggcaagatcc
     4561 tgtcgattga tactgaaggt ctgaccgctg agcaaatccg ccgcggaaaa accgtagttg
     4621 ttgaaggttg tgaagagaaa ctggcacctc tggacctgat tcgtctcggc atgaactaag
     4681 tgtgtgaatg ccgccgatgg cggcattgct ttttttactt cacggaatat ttcgccacgg
     4741 tcgctttcgc gccatgcgct aataaagaca agtacgcttc cgtcactttt gcagtaaaca
     4801 agctattgtc tggcaaatca tcaccaaaga ttgccttaat cgccagcaat gactggacgc
     4861 gcgctttccc ttcggcacta ctttgtacag ccttctgaat aacaggtaac agtgggtcac
     4921 tgatttctat cggatttccc tgttcatcaa caccaccgac ataacgcatc caacccgcga
     4981 cgcccagcgc cagcagatcg aacttgctgt catgcgccag atgccagcga acagaatcca
     5041 acatccgctg tggcaatttc tggctaccat ccatcgcaat ctgccaggtt cgatgacgta
     5101 acgccgggtt gctatagcgt gcaattaatc ggttagcgta atcttgcaaa tcaacgccct
     5161 gcactttcag cgtcggcgct tgttcctgca acatcaagcc atacgccgca taacgataat
     5221 gttcatcttc catgcagtca ttaatgtgct gatatccagc aagataaccc aaatacgcca
     5281 ggaatgaatg actgccgttg agcatgcgca acttcatctc ttcataaggc agcacatcac
     5341 taaccagttc ggctcccgct ttttcccatt ctggacgtcc ggcaacaaag ttatcttcta
     5401 ttacccactg gcggaaaggt tcacaggcaa cgcccgcagg atcgcccaca ccggtaagtt
     5461 gttcgatttt cgccagcgta tcctctgtca ctgtgggcac aatacggtcc accattgttg
     5521 atgggaaagt cacgttatct tcgatccatt gtgccagttt tacatcaaca gctttcgcgt
     5581 atgaagtgac aacgtcacgc ataacatgac cgttttctgg catgttgtca catgacatga
     5641 cggtaaatgc gggaagtcct gccgctttac ggcgggccag cgcctcaaca attacccctg
     5701 ttgctgtttt cggctggtgg ggattttgca cgtcggcagc gaccatcggg tgatcgagca
     5761 ttaactgtcc ggtcgccgga gagtggaaat accctttttc ggtgattgtc agagagacaa
     5821 tcgcgatttg cggttcacac atcgcagcca acacggtttc taagccatcc atctgtacgt
     5881 gcaaggcttt tttaacgacg ccaacgacgc gagccgtcca cgcatcggcc gacatttccg
     5941 caacggtata aagattatct tgctgttgta aatcggcaat ttgctgttcg ccgccgatta
     6001 agttgacctc ataatatccc cagtcactga aatgttctgt agcaagaata tcggcataca
     6061 cgccctgatg cgcacggtga aatgcaccaa agcctaaatg aataattctt ggggccaggt
     6121 tattacgatc ataaacaggg agtgtcgctt ttgctgataa caaattattt cccataacaa
     6181 ttccttaaat ttaaatatgg caagctatat gttttgttat atgaataaaa atcccctctc
     6241 cggtaagaga agggattgag ggtttacaga cttctggaag gttgcgcagc tcttacaaca
     6301 cgcggttgat cttccgcagc gtcttccagc gcacttaaat cacggtcttt cacctctggc
     6361 attttcagcg cagagattaa accaattact gaatatgcca tgatcataat ggcgatcgga
     6421 taccaggatt ccgtcatggt gcagaaaata cccgccagga taggaccaaa accggaagcg
     6481 ataagaccac caatttcttt agaaatagcc atccgggtaa agcggttttt acagccgaac
     6541 atttctgcca tggtaatgtt ttccagagca aataatccca gcaccgcaca gttatgaatc
     6601 acaatcagtg caaccataat ggtgctcggg gcatagcttt tatctacaat gatagaaagc
     6661 attggccatg ccagcacaat cgcggaggta ttcataataa tatacgggat ccggcgacca
     6721 attttatcgg ataaccaacc aaggaacgga atggtcataa agccgagaat cgaactaatc
     6781 atcaatgcat ctgttggaat tgctttgtta aacaataacg tctgcactaa atagcctgca
     6841 aggaaagtct gaattaaccc ggagttaccc gcctgaccaa aacgcagccc tgttgccagc
     6901 cagaaggatt tgctctggaa catgctacca gcaggtgcag gttttgctgt gggttggttg
     6961 ctgtcattaa ccttctcaaa gaccgggctt tctttcagat tcatacgtaa ccagatagca
     7021 aagaccatca cgacaacgct cgccaggaac ggtatacgcc atccccacgc cagcagttcc
     7081 tctttactga gaatgaagaa cataaaggcc cagattgccg ttgcgctcaa ggttccgcag
     7141 ttagttccca tagctacaaa tgaggagata attccgcgct taccttttgg tgcatattcc
     7201 gccagcatcg taccggcacc ggaaatttcc gcacctgcac ccaacccctg aataatacgc
     7261 agcgtcacca gcaagatggg ggcaaaaaca ccaatctgtg cataggtcgg taacacacca
     7321 attaaggtgg tacagatccc catcatggtg atggtaataa agagcacttt tttacgccct
     7381 attctgtcgc ccattttgcc gaaaataaat gctccgacaa tacgcgccac ataacctgca
     7441 ccgtaggttc ccattgccaa aattaacgcc attgccgttg atgattcagg aaaaaatatt
     7501 tcatgaaaca ccaacgctgc gccgagcgaa tataactgga aatccataat tcacaggtgt
     7561 tttttcccat cctgtggttt ccttggcgtt ttctaggttt tttcagatag ttgcattttt
     7621 taaaaagcat cctaagttcg atctcagtgt ctatctgggg cctatttctg tcccatatat
     7681 gccccaaaaa aactccccaa cagataagta gttttttcat ggatttatgc gtaaaatcaa
     7741 gaacggctgg aaatcattca atactcacac tatcgaaaaa tttaccagcc aaccgcagca
     7801 cgttcttgca taaggcgtgt ctgcggtttt tcaactattc agatacatca ctcccatcac
     7861 attcattcct ccgcatcaaa ggcatatagg ctatatcacc ttgatatttt tctttttcag
     7921 ataaaaactg ttatctatgt atacttttaa acccaatccg tgtagagtct ctgcataaga
     7981 tagtttgcag ttgccgcttc agcttgcgcc ataa
//
LOCUS       DAPHJE010000161         8005 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPHJE010000161 DAPHJE010000000
VERSION     DAPHJE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.163"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1345)
                     /locus_tag="RH222_004021"
     CDS             complement(<1..1345)
                     /locus_tag="RH222_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3798958.1"
                     /translation="MYSLKQLVISGRKRRWILMDLETSLPLLYPLRYHIDHLAFRSLS
                     TQSASLQSVKFFYEFWRQKYGVSFCYSFYSSDHNPDIAVGEMPAFWMYLENGHNVQSN
                     VLSLTRVTKANSLTHTVRVRAVIHFISFLINTYISPAYRDDSPKALSLLASRLHTRLQ
                     LCRENYRTLTSNKFSQHSHSSQGFQSLSGAMVLSLYGIITPSSAQKHNPLNPFPSGHL
                     QFRNFLIIRLLLNYGLRTGELLLLECSSIKPNLKGDKFSLIVTTVDEAYEPRKNAPSL
                     KNAWANRVLELDKQDYIFLSIYIAKLRPKTDKHDFIFTSSQQSAKPLSYTSVHSIFSK
                     IDQVFTNQYPEYKSPLYSDSLQRLTPHTTRHTWAFLTLQKIWHLKYLKSQQNKTHFIA
                     EVPSLSGIMEEAKDELRLMGGWSPTSQMPDLYAKRFLSEQANAANVQRIIQDNAAL"
     gene            complement(1593..2915)
                     /gene="hipA"
                     /locus_tag="RH222_004022"
     CDS             complement(1593..2915)
                     /gene="hipA"
                     /locus_tag="RH222_004022"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV3798959.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            complement(2915..3181)
                     /gene="hipB"
                     /locus_tag="RH222_004023"
     CDS             complement(2915..3181)
                     /gene="hipB"
                     /locus_tag="RH222_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV3798960.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3390..4790)
                     /locus_tag="RH222_004024"
     CDS             complement(3390..4790)
                     /locus_tag="RH222_004024"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3798961.1"
                     /translation="MNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVK
                     GDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGK
                     GNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFS
                     HRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDL
                     AQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGIYATWYQNDANKT
                     GAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWY
                     VQPQVQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQ
                     PYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGD
                     KGYSDTQGMLGVKYSW"
     gene            complement(4787..8005)
                     /locus_tag="RH222_004025"
                     /pseudo
     CDS             complement(4787..8005)
                     /locus_tag="RH222_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT         1943 a         2106 c         1752 g         2204 t
ORIGIN      
        1 gtagcgctgc attgtcttga ataattcgtt ggacattggc agcattagcc tgctcagaca
       61 ggaaacgttt tgcatataga tcaggcattt gacttgtcgg actccatccc cccatcagtc
      121 gtagttcatc tttggcttct tccattatcc ctgagaggga agggacttca gcaataaagt
      181 gtgttttatt ttgctggctt ttcaaatatt ttaaatgcca tattttttgc aatgtcaaaa
      241 aggcccaagt atgtcgggtt gtatgcggag tcaatcgttg caacgagtca gagtacaacg
      301 gtgatttgta ctctggatat tggttagtga agacctgatc tattttcgaa aaaatagaat
      361 gaactgatgt gtaggacaaa ggcttcgctg actgttgact cgatgtaaag atgaaatcgt
      421 gtttatcagt ttttggtcga agctttgcta tataaatgct taaaaaaata taatcctgct
      481 tatcgagctc caacacacga ttagcccatg catttttgag agatggcgca ttctttcttg
      541 gttcgtatgc ctcgtcgaca gttgtcacaa tcaaactaaa cttatcacct tttaaattag
      601 gtttaattga gctgcactct aacaacaata gctcacctgt tcgtaaacca taatttaaca
      661 acagacgaat aatcaaaaaa ttgcggaact gaagatgccc ggaagggaaa ggatttagcg
      721 ggttatgctt ttgggcagaa gagggggtta ttattccata caggctgagc accattgccc
      781 cagacaaact ctgaaatcct tggctgctgt gactatgttg cgaaaatttg tttgatgtaa
      841 gcgttcgata attctcccgg caaagctgta aacgggtgtg taaacgcgaa gccaaaagtg
      901 ataatgcttt gggagagtca tcccggtatg caggtgatat gtaggtgtta atcaaaaaag
      961 aaatgaaatg gatcacagca cgaactctaa ctgtatgagt taagctattg gcttttgtga
     1021 ctctcgttaa agacaacaca tttgactgga cgttgtggcc attttctaga tacatccaaa
     1081 atgcgggcat ttcaccgaca gcaatgtctg gattgtggtc agatgaatag aagctgtagc
     1141 aaaacgagac accatacttt tgcctccaga attcgtaaaa aaacttcaca gattgaagtg
     1201 aagctgactg tgtggacaat gagcggaaag ccaaatggtc aatatggtac ctaaggggat
     1261 acagcaacgg cagagaggtt tccaagtcca tcaggatcca tcgcctttta cggcctgaga
     1321 taaccaactg ctttagtgaa tacacaatat ttacctcaaa attatcatgg atatgaagag
     1381 aatgtactct gtacctaatg caagaaacga gagggagatc agaaatccta acgtattgat
     1441 ttatcagtta gtgggagtgt catatgaact tatttaccac agtaggtgat ttaacataat
     1501 atacattatg cgcaccagta taaaccaagg aagaatccag tcgttggcgg tcatgattgt
     1561 cgtgctcatt aacaacgacc aaaccccata tctcacttac taccgtattc tcggcttaac
     1621 cgtccatgca acctcaacac attggtttca actgccgtca ccacgttctc cggaaaatct
     1681 gtcggtaatg aagtcttcac gttatccagt gctgccggaa tcattctggc aaagtcactt
     1741 aggatttcat gcatctgcac ttccgggaat ctcagcacct ttgctgtcgc caaaaaatgt
     1801 cgcggataaa ttttatcgat tgccgttttt ttgcctttgg atgcgttaag ccccattgcc
     1861 agtttgagat cgctgatgtg tattcccgta ccgccaagga ccggaaatgc tgaaatgatg
     1921 tcgtaaaatg gcgtgagtcg ataactgccg ccagcctgaa taaatacgga gaagtttttt
     1981 gcatggccat ccgttgcgcc aatcaaccac tggaagacct ggaatttcat aaaatcataa
     2041 cgatcgcgaa gcgcctcgct ggaccccatc aaaaacgcca tgatccgcgc gatgcctggg
     2101 cctccatctg attcatattt cacagatgaa ggtaaaccga atgtctgaca catatcctcc
     2161 tgtggcaagc gaagtaaaac cgttcgctca gtattccaac gcctgtcaaa acgttcgacc
     2221 gctaacgcgc gcacccttcc cgctttaatg atttctgcgt ccggaacatt caacccaagt
     2281 tctttcgcca gcagcagaca gtaatactca ttatcaacgc tttggctgag atcgagcgtc
     2341 gcattgggct gcctgatttc gccaatcggt aatttaatga tgtgcgtcgt cggcgttatt
     2401 cctttcggaa tgcaccagtc attgcctatt ctaagcagcg ctgtcttctc ctgcgcgcca
     2461 gcaaccgaga tgcgaaagtc attttcttct ctaatcatgc ccagagggat atctgcttta
     2521 taagccgtta atacttcttc aagcctggct tcagtaagct tttcccatgc cattatcggg
     2581 cgcgttatgg tttcattttc gggtaataac gttacggcac caacgctgtc tcgccctatt
     2641 tctgacaata aatcaaacgg ttgtctggat ttagcatgat aacgtttaac gatccggtca
     2701 cgtacaatcg ggctatcggg taacaggtta tcgaagaagt taaatacggc atctgaggtg
     2761 atattccccc tctgcaatgg cagcgaaagt gacaacggtc tggcataacg gctggctaac
     2821 cactccggtg catacttaaa ggtgtgcgcg ccgttggcta acttcgttaa ctcgcctacc
     2881 cgctggttgt tcatccaagt gacaagttta ggcattacca ctccagatct tgctgttctg
     2941 ttgattctgg cgaggcattt ttcgtgtcgc atagtgtcat tgagagttca agcgactgta
     3001 aaatcttaaa aaatgtcgtg agcgtggtat tgtcagggtt gttttcgaaa ttggaaattg
     3061 tcgcctgctt aatgccaatt tttttcgcca gctcgctctg cgtccagcca ttttgctggc
     3121 gaaccagttt cattgcattc gctaattgcg ttgggctata gatcttctga aagctcatca
     3181 tgtcatacgt ccacagcaaa tttatccgct taaggggata ttatcagttt tatcctttag
     3241 tgaggataaa gtcaatctgg aaacacatct tatccgctgt acgggataaa cgctgcgata
     3301 tccgcgatca cggataaaag ggaggcttta aaacctttga agtcggcaat attaagccgc
     3361 atgccatctc gacatgcggc ttatacggtt taccagctat atttcactcc cagcatgccc
     3421 tgagtatcgc tatagccttt atcacctagt tgcacaccga cattccccca caggctaagg
     3481 ttgttattta ctttcgcctc aaccccggta cgtacttcac cgagattacg cgcaccatca
     3541 cggcttacgg tttgaccatt catcttcacg gcgtagactt tgctattgtt gatccagttc
     3601 gcttcaatgt aaggctggaa ctcacgctgt ttaccatcgt cacgctggtg atggctgttc
     3661 aggtaggttt tcaccccaag tcgcgtttgc acgtttccgt cgccttccgt ttcaatgcgt
     3721 gttccgtctt tccgggtatg gtcagaatct ttcacaccca tccaggtgat ttgcacctgt
     3781 ggctggacgt accaggtatt cagcgtccct tcgctgccgc taaatgttcc cgcttcaaag
     3841 gtatacccac cctcaacaga ggccgtcaca ccgcgagaat catagtcgtc agcagaacgg
     3901 ttatcggaac tgacgctgtt atcaaaccag ttatacagcg cccagctgtc aacataagcg
     3961 ccggtcttat tcgcatcgtt ctgataccag gtcgcgtaaa tcccagcgct gtacccgctg
     4021 atgcgcccat ccgatttata acccacacga ttactctgag tattactgtg ctgattggcg
     4081 tagcctgcca tcacgccaag atgccagcga tcctgcgcgt tgctactcca ctgcgccaaa
     4141 tcgccgccta gctgcaatac atagcggtta gcctgagtat ttagctgacc gtcaccggcc
     4201 cttgaacgtt cgtgccctcc gacatgacgc atccacatac tgcttgccga cccctgagaa
     4261 tgcagtgaat ctgtatactg cggctcaccc aaacggtcgt gtaaacgatg gctaaacagc
     4321 gagttggctg cggcaatgtt gctgatatag cttccggcct ccgggcgata aactgatggg
     4381 ccttccggat ccacaacagg gggattattg atggggtcgg gtgtatccgg tggcgttacg
     4441 ccgtcccatt tactggtcag ataccagttt ttctcgtcat tccccttccc tttagccagc
     4501 gtgtagacgt aagccccagc ttcgacagtt ccggttgtca gcgcgaagtt acctgcagaa
     4561 ttaccgccaa cctcaatgag ttcaataccg ttgaccgttt gtgcaccgac gccgccaatg
     4621 ttatcaaccc gaacgcgagt gttcccttgg gtatcgcctt tcacgttcat cttatcggtg
     4681 ggcgaattat cgccgcccag cgtcgcgttg aacgtcatca ggccattgtt tccggtatag
     4741 ttgccgttaa cggtcagtac attccccggt ttcccccctt cgctattcat ttgaaccgtg
     4801 ccgccattct ccaggttgcc gccgattgtg gttttagcga cacgcagtgt ccccccaggc
     4861 ataacgttca catcacctgc agtggtgaca tttcccgaca tcacaccgct gttatggata
     4921 ttaatgtgtt gacttgccat attaatggca gagtcggaac cgaaagcaat ttcccccccc
     4981 acgatatccg tcgtaccctg atacgccgcg gaatcgttgt taagagtcag gatcccggta
     5041 cccagttttg tcacatcacc gctaccgctg atactgtgcg tcatcgccca gctacgctca
     5101 gtggagatgg tgagcaggcc attattgata acggaagcat cacccaggtt tttctgctcg
     5161 ttgacgctga gcgcgctgcc tgtatcaatg ttgaattgcc ccacaaagcg gctattatcg
     5221 ccatccagct ctacatctgt cctggcggtt gcgctcacga tacccttccc gcttatgcta
     5281 ttacgcagtt cgccagtcac gttttttagc gttaacagac cgtcattggc aatatttcct
     5341 ctgcctaacc cctgagtatt atcgaggctg acttccgcat ttgggctaat gctggttaac
     5401 gcattgtagc gtgcattaag cccctcgata gccagcgttc cccctgtaac attcaggttt
     5461 ccgccacctg tcagttcacc ctgactgatc ccacctttat ttaccgtcag cgccccctct
     5521 ttgaacaaaa cagtcgaacc catctgcccg gtgaatgtct ctaccgtctg cgtcgatcca
     5581 ttaagatcga cgatggccgc gttgctgatg ttcagttccc gggtgttgcc aagtgcacca
     5641 tccgcatcgg tacgtaatgt ccccatctga acgtaggttg ccccctgata gtcgttctga
     5701 ccgttggaaa gcgaaacctg tcgcaccgta ttgattgcca gatccccttc accaccgatt
     5761 tttgccgaca tatcggccgt tgcgccatag gctccgccat gttcggctaa cgtcagcttt
     5821 tgcccaccat ggatgttcag cgctttcagc ccatagttaa cgtacaaacc atcacctggt
     5881 gcggtcgtga ggcggaatcc ataatcaccc tcggcgacca ccgttccgtt ttgcgcaatg
     5941 tgtaacgttt tgtccgcttc cacctcgtcg ccctgtaaat cacgtaacgt taatgagcca
     6001 cccgacccaa taaccgtttg cgccttcacc agttgaacgc cgacatggct atcgtcgtgt
     6061 tccagcaaat tgagcgtcgt cagagggtta ttcgccatgg gatcattcca cggtttaggc
     6121 acgtcgataa gcacgtcgcc cgtcccgttt acctgatagt tacggccttt ccaggtgtag
     6181 tcacccgcgc cgacaaccag cgtatcgacg ctgatatatc cctctgcaag cgtcgcagca
     6241 ggaatatccg tatcgaaaat gagcgtaccg ccattcatgg ccagtccacc aatggattgc
     6301 tctcccactt ttaccgatgt ggtattttca ctgtcagact gcaacatcgc gtgagtaagc
     6361 gcagcggtgt tgtcgcgttc cagagtgaag gtactgtctt tcagttgggc aacaccggcg
     6421 aattcagtcc ctgttgcatg ggtaaagcca aacatcttgt cggaggatga cagctgcact
     6481 cgcatcaaac catcgccttt gagcgcattg gtcagcgtgt aatctcctgc actgttcgtt
     6541 gatgcgagaa tgtcgagcgt accggggctt gcaatggtga cttcactcga acccatgccc
     6601 gttaccccat ctgccgtatg agacgcaatg gtcaggaggc cgtcattgac cagcgtttta
     6661 ccgctataag tattccctgc attgaacacc gtatggcctg catcatggat cacttttccg
     6721 tctttatcgt cacccgtaat cagcatatcg acctggtagc cggcatcact gttattagta
     6781 tgattaaaga caaaaacacc ttcgccagaa ccaaactcta ctttcgtcgc gttggttata
     6841 tacccggcat ctgccgccgc ctcaccgtga gctgcaccaa tgtttagcgt tcctacagca
     6901 ggttcaaaaa cacctaagta aacttctcca ccttcgacat tcagcgtgcc gctattcgtc
     6961 agggttaacg tacccgtacc agatgtccct acgtacatat tgaatgtttc aagaagagag
     7021 ttctgcccat ccaccctcac gtcgccctta cttttgtcat tgagagctat ctgtgtatca
     7081 cgcgctttaa ctatacctcc ggtggtaata ttcagttcac ccgtgcctaa tacaccgacc
     7141 tgtagcaatt gggcgttagt agatgatgtc tttaaattcc agagactatc cgttgaaata
     7201 ttaactatcc ctttaccgga ggcatttcct ccgataaatc catagcccgt tgaattaagg
     7261 gtggcaccat tactgacatt gacgataccg ctaccaaaat taccgacatt gatacccaca
     7321 gtatcgaacg atgatgtccc atccatcagg ttcagtgtgc cgataccgct tgctttatcg
     7381 cccaaaaaag taatattttg cgatacaaac ttaccgccat cgctaatatt gaggtttccg
     7441 acgcccgcaa caccaatggt ggtatagaca ttcttattga cctcccacat cccccccgtg
     7501 gtgatgctga cattcccgac gccgttttct ccataaccga gtgagcttcc tccgttgctg
     7561 acaacaagcc cctcattact gatattcatt tcgccgtggc cgtcataacc aagattagtt
     7621 ccgagattag tgataacgga gttcttatcc ttaacagtaa tgttgcctgt gcctgtttct
     7681 ttcatccccg cagtgataat tcccgaatct actttacctt cacgcgagac attaagttca
     7741 ccgtcaccag catcaccgat atagatatag cggaaagctt cgcctgttcc gaggaaactc
     7801 caatgccctt tatcggtcac gttgacgaca ccgtgggaac cgtcttcaac gcccaccaat
     7861 gaatattctt tgttattaat tagtccatta ttggaaatat tgactgcgcc attaccgaaa
     7921 tatccattat agagtctgcg taccgtgatg acagagtctt gatcctgcac attcagggtt
     7981 ccgacaccgg tggcattgcc accga
//
LOCUS       DAPHJE010000162         7937 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPHJE010000162 DAPHJE010000000
VERSION     DAPHJE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.164"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(183..1376)
                     /gene="tyrB"
                     /locus_tag="RH222_004026"
     CDS             complement(183..1376)
                     /gene="tyrB"
                     /locus_tag="RH222_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV3798962.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1629..2708)
                     /gene="alr"
                     /locus_tag="RH222_004027"
     CDS             complement(1629..2708)
                     /gene="alr"
                     /locus_tag="RH222_004027"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV3798963.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2761..4176)
                     /gene="dnaB"
                     /locus_tag="RH222_004028"
     CDS             complement(2761..4176)
                     /gene="dnaB"
                     /locus_tag="RH222_004028"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV3798964.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4259..5242
                     /locus_tag="RH222_004029"
     CDS             4259..5242
                     /locus_tag="RH222_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV3798965.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5408..5650)
                     /gene="pspG"
                     /locus_tag="RH222_004030"
     CDS             complement(5408..5650)
                     /gene="pspG"
                     /locus_tag="RH222_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV3798966.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5784..6821)
                     /gene="dusA"
                     /locus_tag="RH222_004031"
     CDS             complement(5784..6821)
                     /gene="dusA"
                     /locus_tag="RH222_004031"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV3798967.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7185..>7937)
                     /locus_tag="RH222_004032"
     CDS             complement(7185..>7937)
                     /locus_tag="RH222_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDV3798968.1"
                     /translation="KNVFLIEQITNLKVWVNKYIDDCTDEDLNDRDFIASVVDRAIFH
                     FAINSICNPGDNKDATPIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGS
                     GFLTFVNACQEHDDNSLKLFASLLISLSYSSAYTDLAGAEKVNINEYNENYLTAQFEE
                     LSQRDMKKYLGEMKHLADRGGMKFDDYLDKMSLLVNEGKLEPDILSKMRDAAPKLIDF
                     AKSFDPNSKEKIKILTDTSNLIYDLFGVKSAK"
BASE COUNT         2060 a         2058 c         1884 g         1935 t
ORIGIN      
        1 ccaaaaaaac aggataagga attccagata attaggataa gcttaacaat aaaagaacta
       61 ataacgttta ttatctctca cgtagaacga tggcatcaaa aaaatcatct ataaagaaag
      121 agtgaaagcg acgacagttt aatttcactg caggctgggt agctccagcc tgctttcctg
      181 cattacatca ccgcagcaaa cgcctttgcc acacgttgta catttgccgt atttaacccg
      241 gcgacacaca tgcgaccgct ggcgatgaga tagacaccaa attcttcacg tagtcggtca
      301 acctgagcgg cacttaaacc ggtataactg aacatgccgc gctgattaag cagataatcg
      361 aaattgcgtt ctggcatctc tgtgcttaat accttcacca attcctgacg cattgccaga
      421 atgcgagtac gcatctcttc tacttccgcc agccagctgg ctttcaatgc ctcgtcattc
      481 agcactgcag ccaccacctg cgcaccaaaa ttcggcgggc tggagtagtt gcggcgaact
      541 gttgctttca attgccccag tacgcggcca gcggcttcgg catcttcaca cagaacagaa
      601 agtccgccga cgcgctcgcc gtaaagggag aaaattttcg agaacgaatt gctcaccaga
      661 gcgggtaatc cagcgctggc aatggcgcga atagcgtagg catcctcttc cataccggca
      721 ccaaatcctt gataggcaat atcgaggaat ggaataagct cgcgggcttt gagaatttca
      781 atcaccgcat cccactgatc attagtgaga tcggcacccg ttgggttgtg gcaacatgga
      841 tgcagcaaca caatactgcg ggcaggtaat gttttcagcg tcgccaacag gtcattaaag
      901 cgcacgccgt tagtcgcttc gtcataccag gggtaagtac tcacttcgaa tccagccccg
      961 gcgaatattg ctacgtggtt ttcccaggta ggatcgctga cccagacgcc tgattccggg
     1021 aagtagcgtt tcaggaaatc cgcgcccact ttcaatgccc cggagccgcc aagggtttga
     1081 atggttgcta cgcgctgttg tttcagtacc ggatggtccg caccaaacag cagcggcgca
     1141 atggcatggc gatagctgtt aagcccttcc atcggtaaat aaagcgaagc gccatgaggc
     1201 tgcgcattca ggcgcgcttc cgcctccgcc acggctttca gttgtggaat aattccgtct
     1261 tcgttgtagt acagaccgat acttaaattc actttgtcgc tgcgagggtc ttctttaaaa
     1321 cgctccataa gcgtaagaat cgggtcgcca gcgtaggcgt caactttttg aaacacgcga
     1381 tggttctcca ggtttacggg caggtggtta aaacacaata aaccggaaga aggcgaagat
     1441 cgagtggatg ttcaggagcg aacggcaatt agcaacagag tgagactcat gacaaacgta
     1501 catccgccag aggcacgacc tttataagag catgaaagaa catcaactta ttgaattttt
     1561 aggattttat tggccggata aggcattcac gccgcatccg gcacagacaa tcaaatatta
     1621 cagaacgatt aatccacgta tttcatcgcg acccttgaag tcaggcgcgt aataagttcg
     1681 taagcgctta ctttcgtcat ttcagcgata cgttctacgg gcaaaccttc gccccataaa
     1741 atgaccggat ccccggcttt gtcctgcgct tgtggaccta agtctacgca gatcatatcc
     1801 atcgccacgc gcccgacaat cggtacttcg cgaccgttca ccagcactgg cgtaccggac
     1861 ggcgcggcgc gcggataacc atcgccatag cccatcgcga ctacgccaag acgggtatca
     1921 cgttcgctta cccaggttcc accataacca acaggttctc cggctttatg ctcacgcacg
     1981 gcaatcaggc tggaggttag tgacatcact ggctgacagc caaaatcggc accggtggag
     2041 cgatcttcca gcggcgagac gccataaaga atgatgcccg ggcgcaccca gtcaaaatgc
     2101 gactgtggcc acagcagaat gccacccgat gcggcaatgg aacgttgacc aggtttgcct
     2161 tcgcaaaagg tattaaagat agcgagttgt ttctcggttg cgccacattt tggttcatcc
     2221 gcgcgcgcaa aatggctgac gatattcacc ggctgacgaa cgtttttgca ctgggtcagg
     2281 cgatgataaa acgcctcagc ctgttccggc cttacgccca gacggtgcat accggtatcg
     2341 agtttcatcc agacggtaac cggctcgtcc aggctagcct cttccagcgc agccagctgt
     2401 tcttcgttat gcacggcggt atgaaaatgt tgcgcagaaa tcgtcggcag atctctggca
     2461 tcaaaaaagc cttcgagtaa cagtacaggt ttggtgattc cccccgcacg cagtcgcaga
     2521 gcttcttcga gacgggctac gccaaaggcg tcagcatcgg ggagcgttcg cgcggtctca
     2581 agaagaccgt gaccataagc gttcgctttc accaccgcaa ccattttact ggcaggggcc
     2641 agttcacgaa gacgttgcag gttgtgtcgc agagcgcggc ggttaatcac aacagttgcc
     2701 gcttgcattt gtgttccttg ataagtgttt gctttaatta cctaattcat aaaataatta
     2761 ttattcgtcg tcgtactgcg gccccgcata gttgtcgaag cgcgaccatt gaccgttaaa
     2821 ggtcaggcgt accgtcccga ttgggccgtt acgttgttta ccgataataa tttccgcgat
     2881 gccttttaaa tcactgtttt cgtgatacac ctcatcacga tagataaaca tgatcaagtc
     2941 cgcatcctgc tcgatagagc cagattcacg caggtcggag ttgaccgggc gtttgtcggc
     3001 acgttgttcc agagaacggt tcaactggga cagcgccacc accggcacgt tcagttcttt
     3061 cgccagtgct ttcagcgagc gggagatttc tgcaatttcc agcgtacggt tatcggaaag
     3121 cgccggtacg cgcatcagtt gcaggtagtc gatcatgata agcccgatgc cgccgtgttc
     3181 acgggcaata cggcgtgcgc gggaacgcac ttccgttggc gtcaggccgg aggagtcatc
     3241 gatatagata ttgcgttttt cgagcaaaat acccatggtg ccggaaatgc gcgcccagtc
     3301 ttcatcatcg agctgcccgg tacggatttt agtctggtca acgcgcgaca gcgacgccag
     3361 agaacgcatc ataatctgtt ctgaaggcat ctccagcgag aagataagta ctggtttatc
     3421 ctgcaacatc gccgcgtttt cgacgaggtt catcgcaaat gttgttttac ccatcgacgg
     3481 acgcgcggcg acgatgatca aatccgacgg ctgcaagcca gcggtttttt tgttgagatc
     3541 gtcataaccg gtgtttactc cggtaacgcc atcgtgtggc tgctgaaaca actgctcaat
     3601 acgcgccacg gttgcgtcga gcacatcggc gatgttcttc ggcccttcgt ctttgtttgc
     3661 acgactttcg gcaattttaa agacgcggga ttcagcaagg tccagcagat cttcgctggt
     3721 acgcccctgc ggatcaaaac cggcttcagc aatctcattc gcaaccgaga tcatctcacg
     3781 gacaacggca cgttcacgca cgatgtcagc ataagcactg atgttcgccg cacttggcgt
     3841 attttttgac agctctgcca gataagcaaa accaccgacg ctatcgagtt gcccctggcg
     3901 ttccagcgat tccgcaaggg taatcagatc gataggacta ccgctttcct gcaaacgcgc
     3961 catttcagta aagatatggc ggtgtgggcg ggtgtaaaaa tcgtctgcca ccacacgctc
     4021 ggctacatcg tcccagcgtt cgttatccag cattaaaccg cccaacaccg actgctccgc
     4081 ttcgatcgag tgcggaggca ctttcagccc ggcaacttgt ggatcgcgtt cgcggggttc
     4141 agcctgctgt ttgttgaagg gtttatttcc tgccatagtg aatggagtta ccgagataaa
     4201 gaatgggtcg aaactttacc atatgaagca gaccctgacg atacgttctg gaggacacat
     4261 ggcaacacga attgaatttc acaagcacgg tggcccggaa gtacttcaag ccgtagagtt
     4321 cactcctgcc gatccggcag agaatgaaat ccaggtcgaa aataaagcca tcggcatcaa
     4381 ttttatcgac acgtatatcc gcagcggcct ttacccgccg ccatcactac ccagcggatt
     4441 aggtaccgaa gcggcaggca tcgtgagtaa agtcggcagt ggtgtaaagc atattaaggc
     4501 aggcgatcgt gtagtctatg cgcagtcggc gttaggcgct tacagctctg tgcataacat
     4561 taatgcggat aaagcggcga ttctgcctgc ggcaatttct tttgagcaag ctgcagcatc
     4621 tttcctgaaa ggcttaacgg tttattatct gctgcgcaaa acctatgaaa ttaaacccga
     4681 tgagcagttc ctgttccacg cagcagctgg cggcgttggc ttgattgcct gccagtgggc
     4741 aaaagccctg ggcgcgaaac ttatcggcac cgtaggaacc gcgcaaaaag cgcagagcgc
     4801 gctaaaagcg ggcgcgtggc aggttattaa ctatcgtgaa gaggatctgg tcgagcggtt
     4861 aaaagagatc accggcggta agaaagtgcg cgtggtgtac gattccgtgg gcagagacac
     4921 ctgggaacgg tcgctggatt gcctgcaacg ccgcggctta atggtcagtt ttggcaactc
     4981 atcaggtgcg gttaccggtg tgaacttagg cattctcaat caaaaaggct cgttgtatgt
     5041 gacacgccct tccctgcaag gctatatcac cacgcgggag gaattaaccg aggccagtaa
     5101 tgaactgttc tctttgattg ccagcggtgt gattaaggtc gatgtcgccg agcagcagaa
     5161 atatccgctg aaggatgcgc agcgtgcgca tgagattctg gaaagccggg cgacgcaagg
     5221 ttccagcctg ttgattccat aaaagaaata gggcttccac ctgggaagcc ctttcttttt
     5281 atagttcggc tgtatgtagg gtacagcacg atgaatctgt tagaggcgca atagtgacag
     5341 atttgattat caattcctat tttgttctaa ggataaaacc ttaggttgtg atcatccgca
     5401 caatccctta gtaacgccag cggtcataac gctgatattt cggcactttt ggtgctttaa
     5461 tcgccttaat aacccacacc accgcaatcg ccagtagtaa ccacggcagc aacttaatca
     5521 tcaatgccag cataccgccg aggaacataa tggccgtcgc cacaaccagc gcggcgataa
     5581 tgcccagcaa cgaaacgccg gtgaccatca gcatgacaaa aaagccaatc acaaaaagta
     5641 gttccagcat gatgctctcc caaatatgaa atctcttgct ggcattacaa gaatcatgcc
     5701 aaaaataatc tattgattta acagcaaaac gccccgcgac ggtgcgcagg gcgtggtgaa
     5761 tttgactact ttttggtgaa aagttaacgc ttatccgcca ccagtttgag cgcgtgttcc
     5821 agcacattaa tgtctgcacc cgctttatgg gcattttcac ttaaataacg ccgccactgc
     5881 cgcgcgccag gaataccctg gaacaagccc aacatatgcc gggtaatatg gccgagatac
     5941 gtcccctggc tgagttcacg ctcaatgtac ggatacatgg cgcgcactac cgccaccgga
     6001 tcggcatcgg tatccgagga accaaagatc tcccggtcta ctgccgccag aatacccgga
     6061 ttctgatacg cctcgcgccc gaccatcacg ccatccatat gttgcaggtg tgctttggcc
     6121 tcttccagcg acttgatacc accgttaatc gacattgtca gatgcggaaa gtcacgcttc
     6181 agttgataca cacgcggata atcgagcggc gggatttcac ggttttcttt cgggcttaac
     6241 cccgaaagcc aggctttacg tgcgtggatg ataaacatct cacactcgcc tttgccggaa
     6301 acagtgttga tgaaatcgca gagaaattca tagctgtcct ggtcatcgat gccaatacgc
     6361 gttttcaccg tcactggaat cgacaccaca tcgcgcatcg ctttcacaca gtcggcaacc
     6421 agctgcgcat tacccatcag acacgcacca aacatgccgt tctgcacccg gtcagacggg
     6481 cagccgacat tcaggttgat ctcatcatat ccacgcgctt ctgccagctt cgcacactgt
     6541 gccagcgccg ccggatcgct accgccgagt tgcaacgcta ccggatgttc ttcttcactg
     6601 tacgccaggt aatcaccttt accgtgaata atcgcccctg tggtcaccat ttcggtatac
     6661 agcaacgtat tgcgggaaag cagacgcaag aaatagcggc aatgtctgtc cgtccagtcg
     6721 agcattggtg caacgctaaa ccgaccactc cagtgaacgt cagttttttc aggcattgcg
     6781 ctggtttggt tgattttttg catttcagaa ttaccgtgca ttttcaaatg tagagattat
     6841 tttatcgata tatcattggg ttatgttatt cagcatcact gttcaggagg ctcaatagcg
     6901 gggtactata ccataacaac aggaagcgcc tgtctcattg caaaagaaaa ttgagatcaa
     6961 atcaaggcat gaagctctca cgaagtgatg gaaataatct tattagccgt tagccttgtt
     7021 aaggacaatg ataaacaatc caggttcgac gataaataaa aaatcacaca ttaaactccg
     7081 gtgatatctc ttcctgctaa tggcactgat agaagaaaaa agaacccaat aagtattagg
     7141 ttcttttatg taatgccttc cataccatcg aagaacttca catattattt cgccgattta
     7201 accccgaata aatcataaat taaattagaa gtatctgtaa gtattttaat cttttccttt
     7261 gagtttgggt caaacgactt agcaaagtca attaatttcg gtgcagcatc tcgcattttg
     7321 cttaaaatat caggttcgag ctttccttca ttcaccagaa gtgacatttt atccagatag
     7381 tcatcaaatt tcatcccccc ccggtccgcc agatgcttca tctctcccag gtacttcttc
     7441 atatcacgtt gagataattc ttcaaactga gctgtcagat agttctcatt atattcatta
     7501 atattcactt tttctgctcc ggctaaatct gtgtaggcac tagaatatga aagtgaaatt
     7561 aacagcgaag caaataattt taagctgttg tcatcatgtt cctggcaggc attaacaaac
     7621 gttaaaaaac cagaacctat tgcttgaaaa tgtatattcg ctaaaggttc attatcctta
     7681 gattcctcat aaaatagctg aaccgtggag ggaaggccat tcttagtttc tacatcaaaa
     7741 gtacatcgtt caatgggagt cgcatcttta ttatccccag gattacatat actattaatc
     7801 gcgaaatgaa aaatagcccg gtcaacaact gatgcaataa agtcacgatc gtttaaatcc
     7861 tcatcagtgc aatcgtcaat atacttattg acccacactt tgagattagt tatttgctca
     7921 attaaaaaca cattttt
//
LOCUS       DAPHJE010000163         7823 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPHJE010000163 DAPHJE010000000
VERSION     DAPHJE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.165"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..479
                     /locus_tag="RH222_004033"
     CDS             <1..479
                     /locus_tag="RH222_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV3798969.1"
                     /translation="DLSFSKLDLINFEHADLSRVNLNKAILQSINLIDSKLFCTWLTN
                     TFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACMFNIRLYRVNFDEASVQGMGI
                     SILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(633..2282)
                     /locus_tag="RH222_004034"
     CDS             complement(633..2282)
                     /locus_tag="RH222_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV3798970.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2433..3782)
                     /gene="ghxP"
                     /locus_tag="RH222_004035"
                     /gene_synonym="yjcD"
     CDS             complement(2433..3782)
                     /gene="ghxP"
                     /locus_tag="RH222_004035"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV3798971.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4328..4792)
                     /gene="soxR"
                     /locus_tag="RH222_004036"
     CDS             complement(4328..4792)
                     /gene="soxR"
                     /locus_tag="RH222_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV3798972.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4878..5201
                     /gene="soxS"
                     /locus_tag="RH222_004037"
     CDS             4878..5201
                     /gene="soxS"
                     /locus_tag="RH222_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV3798973.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5204..6790)
                     /gene="pdeC"
                     /locus_tag="RH222_004038"
     CDS             complement(5204..6790)
                     /gene="pdeC"
                     /locus_tag="RH222_004038"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV3798974.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7220..7501
                     /locus_tag="RH222_004039"
     CDS             7220..7501
                     /locus_tag="RH222_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV3798975.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         2182 a         2018 c         1789 g         1834 t
ORIGIN      
        1 ccgaccttag tttctctaaa ctcgatttaa ttaactttga acatgctgat ctgtctcgcg
       61 tcaatctgaa caaagcaatc ctccagagta taaacttaat tgatagcaaa ctcttttgta
      121 cgtggctaac aaatacattc ctcgaaatgg ttatatgtac cggctctaat atggctaatg
      181 ttaattttaa taatgccaat ttaagcaact gccatttcaa ctgttctatt ttaacaaaag
      241 cctgtatgtt taatatccgt ctctatcggg ttaattttga tgaggctagc gtccagggaa
      301 tgggcatttc tattctccgt ggggaggaaa atatccccat tgatagtgat accctggtaa
      361 cactacagaa attctttgaa gaagattgta cctctcatac tggcatgtca caaactgagg
      421 ataatattaa tgcagtcgct atgaagatta ctgcagatat tatgcaacac gcagattgag
      481 gttaagtcga agaacagcca atgaattgac tttacaagag agataactta tttgttttag
      541 aatcatgaat gttagcccga gctaacctgt taaaggtgca aaagattgca cctttttcag
      601 attacctcgg gcctggttac gtcgctttcg gcttactgat tttcctcaat tagcaacgct
      661 tcaagcaaat cgagatcgtg cagtaatttt tgcagcgttt cgttgctgat ctcccgcgtg
      721 gcgcgcaggt ggtacagttc agcacgttca gaacgcaatg ccgccagacg gaagcgacgc
      781 tcaaggttct cttcctgcac ggaactttca acgtcgttac gtccatcggc gcgacgacgc
      841 aggttaccaa tgacgcgaga actgacctcc gtaagcagct ggttatcgat attctcttcg
      901 gtatccgccg ccagacgctc ctccattttc tggatcgcca caatcgccac ttctgccgtt
      961 gccgctcgcg caatacgttc ttctttcaat tgttgcgaat ggtccgcgac ttcaatgtgt
     1021 tgtagcagaa taggcaacat caccacgccg acaaacagcg agaagagaat gacgccagcc
     1081 gccaggaaca ccagctcata gcgcgccggg aagacgttac catccggcaa gagcagcggg
     1141 atagagagca caccggccag agtgatcgcc ccacgcaccc cggcgaaaga cgcgatcagg
     1201 atttctcgtg tggtccacga accaaactcc atcggcttct tcttcaggaa gcggttgctg
     1261 aactttttca tcgtccacag ccagccgaaa cggaccagca tcagcgccgc atatatcaga
     1321 ataatatcgg taaacagcat ccagatttcg acgttagggt cgatttctgc cgccatcagc
     1381 gacgtctcca gaatacccgg cagctgcaga cctaacagca ggaacaccat gccgttaaag
     1441 acaaattcca gcatcgccca ggtgctgttt gcacgcaggc gcattgccag cggcgcacgg
     1501 cgcatcacac cggagcgggt gatggtcatc ccggcggcaa ccgcagcgag gatgccggaa
     1561 acgccaatat gttcggcaat cagataagaa gcgaatggca gcaagaacag cagaacaatc
     1621 tgcgtcgcgg gttcatcacc gccccagcgg ctgaggaatc gcagcgagcg accatacagc
     1681 cagctcacca caaaaccggc aagaataccg ccaatggcta ctttcataaa ttcgaccgtc
     1741 gcaccgccga cggtgaagat catcgtcccc atcgccactg ccacggcaaa cttcaacgac
     1801 accaggccgg aagcgtcatt catcaacgct tcgccctgca aaatgcccat gatttttttc
     1861 gggatgcgcc cttcgccaac aatcccggag agcgccacag catcggtcgg agaaagtacc
     1921 gccgccagcg caaaggcggg gatcagcgga atgcccggca ccacccagta aataaggaag
     1981 ccaatgccga ccacggtgac caccaccagc gccagcgcga ggccgaaaat ctctcgacca
     2041 tgttcgagaa attcacgggt cggcgttttc cagccatcag cgaacagcaa cggcgggata
     2101 aacaagacta aaaagagttc aggatcaaac tccacatgca aaccaaacgt cggccacgcc
     2161 agtagcgcac cgatggcgat ttgcataagc ggaagcggga tctgaaaggg catgacacga
     2221 gtgaccaccc cggacagcga gaccacgagg gtcattatca gtatggtgaa gaagatttcc
     2281 atgcgttccc tgtttgcgat gttttttatg atctgagaaa gacgttatgc ctgaattctc
     2341 attttttcag aatatcgcaa aagctcacac aatgtgcgga gaaaataaaa acgggcagcc
     2401 attgccaccc gttttcagaa tccgatgaaa gattagatag cccagccacc cgcatagaag
     2461 gtcaccagcg ccacggcgat aacgaccgta ccgatgttca acttgcgcca ttcgccggaa
     2521 accagacgac caatcaccag agtcgcgaag ccgatcatga tgcctgttac gatgttacag
     2581 gtcagcacga tgaataccgc cgtaaccaga cccgccatcg catcaacaaa atcagcaaag
     2641 tcgattttcg ccacgttgct cagcatcagc aggccaacgt acatcagcgc cggagccgtt
     2701 gcataccccg gaacgaggta agagagcgga gagaggaaca gaatcagcag gaacagcacg
     2761 ccaacggtga tagccgtcaa accggtttta ccgcccgccg ccgtacccgc cgcagactcg
     2821 atgtataccg ctgccggagc tgcacccacc aggccagaga aaacgctgct catggagtca
     2881 gtggtcagtg ctttcccacc gtcgataatt tgcccatctt tatccagcag gttcgcctgg
     2941 ccggcgacgg cacggatagt tccggtggca tcaaatactg ccgtcattac cagcgccaga
     3001 acgcttggca ggactacagg attcagcgcg cccataatgt ccaggctgcc aatcagtgag
     3061 ttgccgtttt catcgctcaa tgaaggcatg gcgaaaacgc cggagaaatg gacgttagga
     3121 tcgaagatca aaccgacaat tgagataccg ataatggtca gcagaatgcc accagggact
     3181 ttcagttttt ccaggccgat gatcaccgcc agacctacca gtgacataat caccgggaag
     3241 gtcgcgaaat cacccagcgc aacgggcaga ccatcaagcg ggtttttaat caccagaccg
     3301 acaccgttag cggcaatgag cagcaggaac agaccgatac caatccccgt gccgtgcgcc
     3361 acaccgtgcg gcaagttgcg caaaatccag ctacggatac ccgtggcaga aattaccgta
     3421 aacagcacac ccatcaggaa cacggcaccc agcgcgacag gtacgctaat atgttgcccc
     3481 agcaccaggc tgaatgcggt aaatgccgtc agggagatgg cgcaaccaat cgccaacggc
     3541 agattagccc acagacccat cacgatagaa ccgagtccgg caaccagaca ggttgcaacg
     3601 aaaactgccg caggcgggaa gcctgcttta cccaacatac ctggaacgac gatgaccgag
     3661 tagaccatcg ccagaaacgt tgttaacccg gcaaccactt cctgacggac agtgcttcca
     3721 cgttgtgaaa ttttaaacca ggcgtcgagt gaaccgccgg tacgcgctga tggcgtagac
     3781 ataagaaaac atcccctgag agtttaattt tcgtcaagat gcgtggcgga caatcccctg
     3841 ccagcgaaac gttatcttcc tgctccaggt ttgcgacaat ttgctgcgtc gcaaaaaagc
     3901 aaacgtttag ctccgcgcat acaaaacggg tggtaaaaaa agcaaacgat tatccggcga
     3961 tcatagcggc tttttcaacc gaagtttgct gcttttttct cttttattgt cttgacgatg
     4021 aaaaaccagg caattgatgc gcaaacgtta tcgtcggtgc gcaagattgc agcgttatcg
     4081 agccgtatac cgttcaggat tgcgataaaa aatcgtctga gatgacaaaa atattgcggt
     4141 taaaaagtat acttttacac tggcgaagaa acgtcggtga catacaatta aagcatcaac
     4201 accaaccgga acctccacca cgtgctcgaa tgaggtgtgt tgacgtcggg ggaaaccctc
     4261 ctgtgtacca gcgggataga gagaaagaca aagaccggaa aacaaactaa agcgcccttg
     4321 tggcgcttta gttttgttca tcttccagca agcgtgcgcc ggtaccttct tctcctaagc
     4381 ggtcgcccgg gttacgcaac gggcaatcac tgcgcgaaag gcagccacaa ccaatacatc
     4441 cgtccagttc gtcacgcagc gccactaagg tatgaatgcg ccgatccaac tcttctcgcc
     4501 attgggacga aagctgtttc cactctttcg cacttaacgt atgcccttcg ggcaacacgc
     4561 caaacgcttc accaatggtc gccagcggaa tgccaatacg ctgagcaatt ttgataattg
     4621 caacatatcg caacacatca cgtttatatc gccgctgatt gccgctgtta cggatactgg
     4681 taatcaaccc tttactttca tagaaatgca gcgccgatac cgccacaccg ctgcgtttcg
     4741 ccacttcgcc gggggttagc agcgctttaa tgcggggtaa tttcttttcc ataaatcgct
     4801 ttacctcaag ttaacttgag gaattatact ccccaacaga tgaattaacg aactgaacac
     4861 tgaaaagagg cagatttatg tcccatcaga aaattattca ggatcttatc gcatggattg
     4921 acgagcatat tgaccagccg cttaacattg atgtagtcgc aaaaaaatca ggctattcaa
     4981 agtggtactt gcaacgaatg ttccgcacgg tgacgcatca gacgcttggc gattacattc
     5041 gccaacgccg cctgttactg gccgccgttg agttgcgcac caccgagcgt ccgatttttg
     5101 atatcgcaat ggacctgggt tatgtctcgc agcagacctt ctcccgcgtt ttccgtcggc
     5161 agtttgatcg cactcccagc gattatcgcc accgcctgta attttattgc ccgcgcgtta
     5221 actcccgcgc gggtaattgc tccatccatt gcataaacac ctgcggcggc atcgccttcg
     5281 caaagaacca tccctggcaa tagcgcacgc cgcgtttgcg cagccagtta acctgctcct
     5341 cagtttcgac gccttcagcg atcgttttta accccaggct gtgcgccagc tcgatgatgt
     5401 gttccgcaat caaatgactg gttttgtggg tggtcagcgt ttcaacaaac gatttgtcga
     5461 ttttcaaaat atcgacattc aatgatttaa ggttatgcaa gttagagtag ccaataccaa
     5521 aatcatcaat tgccacttcg taacctgcct ggcggaaagc cagaataatc ggcgtcattt
     5581 tgtcaacatc aagaaatgca tgttcagtca cttcaaattt aatttgctgc ggacgcaccg
     5641 cgtattgctc tgttttctga ttgattcgcg ctatcaaccg tgacgtatga aaatcggagg
     5701 ccgacaggtt aatagaaaca tagcgatctg catgtgttgc caggtaatcg cccagatcgc
     5761 ggaagacatt atcaataaca taatcagtta tctgttctat catcccctcc ttttctgcca
     5821 gcggaataaa ctctgccgga ttcattattt gcccctgctc accaagccaa cgtaacaacg
     5881 cttcagcgcc gatacatttt tctgttttga tatcgattat tggctggtaa taaagacaaa
     5941 gttgatgttt ttcgagggcg cgttgcaatt tacgtttggg agataaatag ttttgtcgaa
     6001 tacgtagcca gagtaatagc agaacaagac tccccaaaat accggcgggc aacgcaaaaa
     6061 taagatgatt ataaaaatgg gttataagac gttgatatga agtcgcaaca atggctgcaa
     6121 ttgggcgttt tgtcgaataa actgtcgcat ataaatagcc atttctttgt acggttaaat
     6181 ccttcaaatg aatcagcgga gaaaacgttg ctgccgaggc ctctttgctt aacgagaaaa
     6241 aggttttcgt caccgtatca tacacacccc attgcaatgt cgggtcatca gacatcactt
     6301 cactccagaa gagagggttg ataaccgcca cataatttcc ccgctgcata taggtcattt
     6361 tatagccaga gaaaaaaggc gtatcgcggt aataatagat agaaacgtta ggttcacgct
     6421 tataatcggc cggtgcaatc gtatagccgt ttacaggcgc tatcagcgat gagcataaaa
     6481 aatggttatc acgggcatag atcaattcat taatataaag atagccacga ataatattca
     6541 acattcgctt ttgatgggct ggagtgcaaa cttgcccctg atagcgttca gctgcatcgc
     6601 tcactaaatc tgcctggcga atgaccagct cggatttatc cagtgccagt tgagcaaagg
     6661 ttcggagctg attattcact tctgattttg cccaaaggaa ggcaatccat agcgaaagaa
     6721 tgatgggaaa gagaactaaa aagataatgc ccggcaacgc cagtaattgg tgtcgtgcac
     6781 gatgactcat tgctatcaat ttcctgcctg tttatacccg gccattgcaa ggtgaaaacc
     6841 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
     6901 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
     6961 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
     7021 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
     7081 tagttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
     7141 gatagccaga gtgtaccacg cctcccgtga acaacgccgc gctgtccagg gcatcggctc
     7201 ttttcagagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
     7261 acttcttagt gccgtaatga tgtttctggc cagcacactc aacatccgtt ttcgtcggtc
     7321 tgattatgtc gggcttgcgg tgatcagcag cggtctgggc gttgtttccg cctgttggtt
     7381 cgcaatgggg ttgcttggca tcacaatggc ggacatcacc gctatctggc acaacatcga
     7441 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacctg
     7501 atacgtagaa gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat
     7561 ccaccttaaa acaatataac ctattgtttt aatgacaaat cagaacggtt cacataacct
     7621 gatacttata aatcgtgaaa aaaaaccttt tttatcagaa actaaggcat aagaaatgat
     7681 ttaactgtaa agaatttagt taaaatataa tctatcaaag tgcataattt tacttttttt
     7741 ggtgacccag ctcggacctt tgattcaatc tggcatagat tttggtaaga gatcaaaaaa
     7801 ccggaaatct caaaaagtgc gtg
//
LOCUS       DAPHJE010000164         7780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPHJE010000164 DAPHJE010000000
VERSION     DAPHJE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.166"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..612
                     /gene="rfbK1"
                     /locus_tag="RH222_004040"
     CDS             <1..612
                     /gene="rfbK1"
                     /locus_tag="RH222_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK1"
                     /protein_id="HDV3798976.1"
                     /translation="DDEASFIEGYYIVGLLAEAFLQKQPGAKIIHDPRLTWNTVDIVT
                     RSGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELLC
                     LKNSSLKSLVADRQAAFPASGEINRKLGNAAEAIARIRAQYEPAAAHIDTTDGISIEY
                     PEWRFNLRTSNTEPVVRLNVESRADTALMNAKTTELLHLLSGE"
     gene            614..1408
                     /locus_tag="RH222_004041"
     CDS             614..1408
                     /locus_tag="RH222_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen ABC transporter permease
                     subunit Wzm"
                     /protein_id="HDV3798977.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFRKHAADMVDEL"
     gene            1408..2622
                     /locus_tag="RH222_004042"
     CDS             1408..2622
                     /locus_tag="RH222_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen ABC transporter ATP-binding
                     protein Wzt"
                     /protein_id="HDV3798978.1"
                     /translation="MSYIRVNNVGKAYRQYHSKTGRLIEWLSPLNTKRHNLKWILREI
                     NFEVAPGEAVGIIGINGAGKSTLLKLITGTSRPTTGEIEISGRVAALLELGMGFHSDF
                     TGRQNVYMSGQLLGLSSEKITELMPQIEEFAEIGDYIDQPVRVYSSGMQVRLAFSVAT
                     AIRPDVLIIDEALSVGDAYFQHKSFERIRKFRQEGTTLLLVSHDKQAIQSICDRAILL
                     NKGQIEMEGEPEAVMDYYNALLADKQNQSIKQVEHNGKTQTVSGTGEVTISEVHLLDE
                     QGNVTEFVSVGHRVSLQVNVEVKDDIPELVVGYMIKDRLGQPIFGTNTYHLNQTLTSL
                     KKGEKRSFLFSFDARLGVGSYSVAVALHTSSTHLGKNYEWRDLAVVFNVVNTEQQEFV
                     GVSWLPPELEIS"
     gene            2622..3899
                     /locus_tag="RH222_004043"
     CDS             2622..3899
                     /locus_tag="RH222_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3798979.1"
                     /translation="MGSSFYRSFEERHRGSVEEIKRRLSFYLPFLAGLKDIYPDGMIA
                     DIGCGRGEWLEILTENGIANIGVDLDDGMLARAREAGLNVQKMDCLQFLQSQADQSLI
                     ALTGFHIAEHLPFEVLQQLVMHTLRVLKPGGLLILETPNPENVSVGTCSFYMDPTHNH
                     PLPPPLLEFLPIHYGFNRAITVRLQEKEVLQSPDAAVNLVDVLKGVSPDYSIIAQKAA
                     PTDILERFDTLFTQQYGLTLDALSNRYDAILRQQFSSVVSRLETLNQTYMQQISQMSE
                     TIQTLQGEVDDLSHVIDQNHQLHQQMADLHNSRSWRITQPLRWLSLQRHLLRQEGAKV
                     RARRAAKKILRKGMALSLVFFHRYPKSKVYLFKVLRKTGCYTLLQRLFQRVMLVQSDT
                     IMMQSRRYDVGTEEMTSRAMSIYNELKNKNTEK"
     gene            3902..7543
                     /locus_tag="RH222_004044"
     CDS             3902..7543
                     /locus_tag="RH222_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3798980.1"
                     /translation="MRIVIDLQGAQTESRFRGIGRYSIAIARGIIRNNSRHEIFIALS
                     AMLDESIADIKAQFADLLPAENIVVWHAVGPVRAMDQGNEWRRESAELIREAFLESLC
                     PDVVFITSLFEGHVDDAATSVHKFSRQYKVAVLHHDLIPLVQAETYLQDDVYKPYYLQ
                     KVEWLKNADLLLTNSAYTAQEAIEHLHLQGDHVQNIAAAVDSQFCMAEVAASEKETVL
                     GHYGIQREFMLYAPGGFDSRKNFKRLIEAYAGLSDALRRSHQLVIVSKLSIGDRQYLE
                     SLASGNGLQQGELVLTGYVPEDELIQLYRLCKLFIFASLHEGFGLPVLEAMSCGAPVI
                     GSNVTSIPEVIDNPEALFDPYSVSSMRDKIAQCLTDDTFLARLKEIAQQQARNFSWDK
                     AAVTALEAFEKIAVEDTGTAQVLPEALIQKILAIPQGQPDDRDLRLCATAIDYNLKTA
                     ELYQIDDKSLNWRVEGPFDSSYSLSLVNREFVRALSADGVEVLLHSTEGPGDFAPDAS
                     FMAQSENSDLLAFYNQCQTRKSNEKIDILSRNIYPPRVTKMDAKVKFLHCYAWEETGF
                     PQPWINEFNRELDGVLCTSEHVRKILIDNGLNVPAFVVGNGCDHWLNIPAETTKDVDH
                     GTFRFLHVSSCFPRKGIQAMLQAWGRAFTRRDNVILIIKTFNNPHNEIDAWLAQAQAQ
                     FIDYPKVEVIKEDMSATELKGLYESCDVLVAPGCAEGFGLPIAEAMLSGLPAIVTNWS
                     GQLDFVNSQNSWLVDYQFTRVKTHFGLFSSAWASVDIDNLTDALKAAASTDKSVLRDM
                     ANAGRELLLQQFTWKAVAGRSCQAVKTLRAHIDIAQHRARIGWLTTWNTKCGIATYSQ
                     HLVESAPHGADVVFAPQVSAGDLVCADEEFVLRNWIVGKESNYLENLQPHIDALRLDV
                     IVIQFNYGFFNHRELSAFIRRQHDAGRSVVMTMHSTVDPLEKEPSWNFRLAEMKEALA
                     LCDRLLVHSIADMNRLKDLGLTANVALFPHGVINYSAASVTRQQQSLPLIASYGFCLP
                     HKGLMELVESVHRLKQAGKPVRLRLVNAEYPVGESRDLVAELKAAAQRLGVTDLIEMH
                     NDFLPDAESLRLLSEADLLIFAYQNTGESASGAVRYGMATQKPVAVTPLAIFDDLDDA
                     VFKFDGCSVDDISQGINRILNSIREQNSWATRTQQRADAWREQHDYQAVSRRLVNMCQ
                     GLAKAKYFK"
     gene            7607..>7780
                     /locus_tag="RH222_004045"
     CDS             7607..>7780
                     /locus_tag="RH222_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDV3798981.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIEQIPLVENKSDT"
BASE COUNT         1919 a         1714 c         2035 g         2112 t
ORIGIN      
        1 gatgacgagg cgtcgtttat cgaggggtac tacattgtcg gcctgctggc ggaagcgttc
       61 ctgcagaagc agccgggggc gaaaatcatt cacgacccgc gtctgacgtg gaacacggtg
      121 gacatcgtga cccgcagcgg cggccagccg gtgatgtcga agacggggca tgcgttcatc
      181 aaggagcgga tgcgccagga agacgccatc tacggcgggg agatgagcgc gcaccattac
      241 ttccgcgact tcgcctactg cgacagcggg atgatcccgt ggctgctggt ggcggagctg
      301 ctgtgcctga agaacagctc gctgaaatcg ctggtggcgg accgccaggc ggcgttcccg
      361 gcgtcggggg agatcaaccg caagctggga aatgcggcgg aggcgatagc gcgcatccgg
      421 gcacagtatg agccggcggc cgcacacatc gacacaacgg acggtatcag tattgaatac
      481 cctgagtggc gctttaacct gcgcacgtcc aatacggagc cggtggtgcg tctgaacgtg
      541 gagtccagag cggatactgc gttaatgaat gcaaaaacga ccgagctgtt acacctgtta
      601 agcggggaat aaggtgagag atttactaac gacgatttat cgttatcggg gatttatctg
      661 gagcagtgtt aaacgtgatt ttcaggcgcg ctatcaaact agtatgctgg gcgcactatg
      721 gctcgtttta caaccgctct ctatgattct ggtctatacc ctggtttttt ccgaggtgat
      781 gaaggcaaga atgcccgata ataccgggtc gtttgcctat agtatttatc tctgttccgg
      841 ggtactgacc tggggattat ttactgagat gctggataaa ggtcagagcg tatttattaa
      901 caatgctaat ctgatcaaga aactcagttt tccgaaaatc tgtctgccga tcatcgtgac
      961 gttatcggcg gtgctaaatt tcgcgattat tttcagtctg tttctaattt ttatcattgt
     1021 caccggtaac ttccccggct ggctctttct ctcggtgata ccggtcctgc ttttgcagat
     1081 cctgtttgcc ggtgggctgg ggatgatcct tggtgtcatg aacgtctttt tcagggatgt
     1141 ggggcaactg gttggcgttg cgctgcaatt ctggttttgg ttcacaccca ttgtttatgt
     1201 actgaattca ttacctgcat gggcaaaaaa tctgatgatg tataacccga tgactcggat
     1261 catgcaatct tatcagtcta tcttcgccta tcatctggcc cccaactggt attcgctatg
     1321 gccagtattg gctctcgcca ttattttctg cgtcatcggt ttcaggatgt tccgcaagca
     1381 tgcggcggat atggtggatg aattataatg agttatatca gagtaaataa tgtcggtaag
     1441 gcgtatcgcc agtatcactc aaagaccggg agactgatcg aatggttatc ccctctgaat
     1501 acaaaacgcc ataatttgaa atggatcctc cgcgagatta atttcgaagt cgctccgggc
     1561 gaggctgtcg gtattatcgg tatcaacggt gcaggcaaga gtaccctgct taaactcata
     1621 accgggacgt ccaggccgac gactggagaa attgaaatct ccggacgtgt cgctgcatta
     1681 ctcgaattgg ggatggggtt tcattctgat ttcactggcc ggcagaatgt ttatatgtct
     1741 gggcaactgt tggggttatc gtcagagaaa ataactgaac tgatgccgca aattgaagag
     1801 tttgctgaga ttggggacta tatcgatcag cctgtgcgcg tctactccag tgggatgcaa
     1861 gttcgattag cttttagtgt agcgacggct atccgtcctg atgtgctaat tatcgatgaa
     1921 gcattatctg ttggggatgc atatttccag cataaaagct ttgagcgtat tcgaaaattt
     1981 cgtcaggaag ggaccacgct gttgctggta tcccatgata aacaagcgat ccaaagcatt
     2041 tgcgaccggg ccattttatt gaataaaggc caaattgaaa tggaaggtga acctgaagca
     2101 gtgatggatt attacaatgc tcttctggcc gataaacaaa atcagtccat taaacaagtt
     2161 gagcataatg gtaaaacgca aactgtttca ggcactggtg aggtgactat ctctgaggtt
     2221 catcttctcg atgaacaggg caatgtgact gaatttgttt cggtagggca tcgtgtcagc
     2281 ttgcaggtca acgttgaggt caaggacgat attcctgagc ttgttgtcgg atatatgatt
     2341 aaggatcgac ttgggcagcc gattttcggg accaatacgt accatctcaa tcagacactc
     2401 acctccctga aaaaaggaga aaagcgttcg ttcttatttt ctttcgatgc gagattgggg
     2461 gttggctcct attctgtcgc tgtcgcgttg catacttcca gtacgcacct cggcaaaaac
     2521 tatgaatggc gcgatctggc cgtggtattc aacgtcgtta acacggaaca acaagagttt
     2581 gtcggcgtgt cctggttgcc gcctgaactg gagatttctt aatgggttcg tcgttttatc
     2641 gttcatttga agaacgacac agaggttcgg ttgaagaaat caagcgccgc ttgagttttt
     2701 atttaccttt tcttgcaggt ctgaaggaca tttatcctga tggcatgatt gcggatattg
     2761 gttgtggacg tggcgaatgg ttggagatcc tgactgaaaa tggcattgca aacatcggcg
     2821 tcgatctcga tgatggcatg ctggcgcgcg ccagggaggc cggactgaat gtgcagaaaa
     2881 tggattgtct gcagtttctg caaagtcagg cggatcagag cctgatagcg ttgaccggtt
     2941 ttcatattgc tgagcatttg ccttttgagg tcctgcagca gctcgtcatg cataccctac
     3001 gggtgctgaa accaggtggt ttgctgatcc tcgaaacgcc gaacccggag aatgtaagcg
     3061 tcggcacctg ttcattttat atggatccaa cgcataatca tcctctgcca ccgccactgc
     3121 ttgagttttt acctattcat tatggtttta accgagcaat taccgttcgt ctgcaggaaa
     3181 aagaggttct tcaatctccg gatgcagccg ttaatttggt cgatgtactc aaaggggtga
     3241 gccccgacta cagcatcatt gctcagaaag cagcgccaac agatattctt gaacgctttg
     3301 acaccctgtt tacccagcag tacggtctga cgctggatgc tctgagcaac cgttacgatg
     3361 cgattttgcg ccaacagttt tcgtccgttg tctcacggct ggagacgttg aaccaaacct
     3421 atatgcaaca gataagccaa atgtcagaga ctattcagac gttgcaaggt gaggttgacg
     3481 atctgagtca tgttatcgat cagaaccatc agcttcatca gcaaatggcg gatttacata
     3541 acagtcgttc atggcgtatt actcaaccac tacgctggtt gtctttgcaa cgtcatttat
     3601 tacgtcagga aggggctaaa gtgcgagccc gtagggctgc gaaaaaaata ttgcgcaaag
     3661 ggatggcgct ctcgctggtc tttttccatc gttaccctaa gtctaaggtt tatctgttta
     3721 aggttctgag aaaaactggc tgctatacat tgctacaacg tttgttccaa cgcgtaatgc
     3781 tggtgcaatc tgacacgatc atgatgcagt ccagaagata tgatgtgggt actgaagaaa
     3841 tgacaagtcg cgcgatgagt atttataacg aattaaaaaa taaaaatacg gagaaataac
     3901 gatgcgtatt gtcatagatt tacaaggcgc acagacggaa agccgctttc gtggcatcgg
     3961 tcgttatagt atcgcaatcg ccagaggcat aatcagaaat aacagccggc atgagatttt
     4021 catcgcgcta tccgccatgc tggatgagtc gattgcagat attaaggcgc aatttgccga
     4081 tctcctgccg gcagaaaata tagtcgtatg gcatgccgta ggccctgttc gtgcgatgga
     4141 ccaaggtaat gaatggcgtc gggagagcgc agaactgatt cgggaagcgt ttcttgaatc
     4201 attgtgtcca gatgtcgttt tcattacgag tttgtttgaa ggtcatgtcg acgatgcggc
     4261 tacatcggta cacaaattta gtcgtcagta taaagtagcc gtactgcacc acgatcttat
     4321 ccccctcgtg caggcggaaa cctatctgca ggacgatgta tacaaaccct actacttaca
     4381 gaaagttgag tggttaaaaa acgctgacct tttgttgact aactctgctt ataccgcaca
     4441 ggaagcgatt gagcatctgc atttacaggg cgatcatgtg cagaatattg cagccgcagt
     4501 cgattctcag ttttgtatgg cggaggtggc agcgagcgaa aaagagaccg tccttggcca
     4561 ttacggtatt cagcgcgagt tcatgttgta tgcgcccgga ggatttgact caaggaaaaa
     4621 ctttaaacgg ttgattgagg cctatgccgg gctcagtgat gccttacgtc gcagtcatca
     4681 actggttatc gtcagtaagc tttccatcgg tgatcgtcag tatctggaat cccttgcgtc
     4741 aggtaatggt ttacagcagg gcgaactggt actcactggt tatgtgcctg aagatgagct
     4801 gatccagctc tatcgcctat gtaagctgtt catctttgct tcactacatg aaggttttgg
     4861 gttgccggtt ctggaagcaa tgtcgtgcgg tgcgccggtg attggctcaa atgtcaccag
     4921 tattcctgaa gtcatcgata atcctgaggc attattcgac ccgtattctg tctcttccat
     4981 gagggataag atcgcgcaat gtttgactga tgataccttc ctcgcgcgtc tgaaagaaat
     5041 agcgcagcag caagcgcgta atttctcttg ggataaggct gcggtgactg ctctggaagc
     5101 tttcgaaaag atcgcggtag aagacaccgg tactgcgcag gttttgcctg aagctttgat
     5161 tcagaagatc cttgctatcc cacaagggca gccagatgac cgcgatctgc gcttgtgcgc
     5221 aacggccatt gattacaatc tgaaaacggc agaactttat caaatcgacg ataaatcgct
     5281 gaactggcgt gtggaaggcc cattcgatag ctcatatagt ctgtcgttgg tcaaccgcga
     5341 atttgtccgg gcactctcag ccgatggtgt agaggtttta ttgcattcca ctgaaggacc
     5401 aggtgatttt gccccagatg cctcgtttat ggcacagtcg gaaaatagtg atcttctggc
     5461 attttataat caatgtcaga cccgcaagag taacgaaaag atagatattc ttagcagaaa
     5521 tatctatcca ccgcgggtta ccaaaatgga tgccaaagta aaattccttc attgttatgc
     5581 ttgggaagaa acgggctttc cgcaaccgtg gatcaatgaa tttaatcggg aacttgacgg
     5641 agtgctgtgt acttcggaac atgttcgtaa aatattgatt gataacggac tgaatgtgcc
     5701 cgcatttgtt gttggcaatg gctgtgacca ttggctcaat atcccagccg agacgacaaa
     5761 agatgtggat cacggaacat tccgtttcct tcatgtctct tcttgtttcc ctcgcaaagg
     5821 gatacaggca atgcttcagg cttgggggag ggcgttcact cgtcgtgaca atgttatctt
     5881 aatcattaag acttttaaca atccgcacaa tgaaattgac gcatggctgg ctcaggccca
     5941 ggctcaattc atagactatc ccaaagttga agtgatcaaa gaggatatgt cagccaccga
     6001 gcttaaaggg ctttatgaaa gctgtgatgt tttggttgct ccaggttgcg ctgaaggctt
     6061 tggtttacct attgctgaag caatgctgag tgggctgccg gctatcgtca ccaattggag
     6121 tgggcaactt gattttgtta attcacaaaa ttcatggctg gttgactatc agttcactcg
     6181 ggtaaaaacg cactttggtc tgttttcctc agcctgggcc agtgtggata ttgacaactt
     6241 aacagatgca ttaaaagcgg cagcctcaac cgataaatca gtgctgcgtg acatggccaa
     6301 tgctggtcgc gagcttcttc tgcagcagtt tacctggaaa gcggtggctg gtcgttcttg
     6361 ccaggcggtc aagactctgc gtgcgcatat tgatattgca cagcatcggg cgcgcattgg
     6421 ctggttgacg acctggaaca cgaaatgtgg gatcgcaacc tattcccagc atctggtgga
     6481 aagcgcacct catggcgcgg atgttgtttt tgctccccag gtcagcgctg gcgatcttgt
     6541 gtgtgcagac gaagagtttg tacttcgcaa ctggattgta ggtaaagaga gcaactatct
     6601 ggaaaacctc cagccacaca ttgatgctct gagactcgat gtcatcgtga tccaattcaa
     6661 ttatggattc tttaatcatc gagaactgtc ggcgtttatt cgtcgccagc atgacgccgg
     6721 tcgttcagtt gttatgacga tgcactcaac tgtggatccg ctggaaaaag agccgagctg
     6781 gaatttccgt cttgctgaaa tgaaagaggc gctggcactt tgcgaccggt tgttggtgca
     6841 ttcgattgcc gatatgaacc gccttaaaga tttaggctta actgcgaatg ttgctttatt
     6901 cccgcacggc gttatcaact actccgcagc gagcgtcacg cgtcaacagc agtctttacc
     6961 gctaattgcg agctatggct tctgcttacc gcataagggc ctgatggaac tagtagaatc
     7021 cgtccataga ctcaagcaag ctggtaaacc ggttcgttta cgactggtga acgcagagta
     7081 tcctgttggg gagtcacgcg atctggtggc agagcttaaa gcggctgctc agcggttagg
     7141 tgttaccgat ctgattgaga tgcacaatga tttcctccct gatgcggaga gtctgcggtt
     7201 gctttcagaa gcagatcttc tgatttttgc ttatcagaat actggggagt ctgctagcgg
     7261 ggcggtacgt tatggtatgg cgactcaaaa acctgttgcg gtaacgcccc tggcgatatt
     7321 tgatgatttg gacgatgccg tctttaaatt tgatggatgt agcgtcgatg atatcagtca
     7381 gggaattaac cggatcctga attccatccg tgaacagaac tcttgggcaa ccaggactca
     7441 acaacgtgcc gatgcatggc gggaacaaca tgattatcaa gctgtttcac gccgtctggt
     7501 taatatgtgt caaggcttag ctaaagctaa atattttaaa taaaaatatc tctcttgtat
     7561 tttttgcctt tgaatacaag aggggttaga taatgtgtca tttattatga aaattatttt
     7621 tgctactgag ccaattaaat acccattaac gggcatcggt cggtattccc tggagctggt
     7681 taagcggctg gcggtcgccc gcgaaattga agaattaaag ctatttcacg gtgcgtcgtt
     7741 tatcgaacag atccctttgg tggagaataa aagcgatacc
//
LOCUS       DAPHJE010000165         7748 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPHJE010000165 DAPHJE010000000
VERSION     DAPHJE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.167"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            173..391
                     /locus_tag="RH222_004046"
     CDS             173..391
                     /locus_tag="RH222_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV3798982.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            609..851
                     /locus_tag="RH222_004047"
     CDS             609..851
                     /locus_tag="RH222_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798983.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1181..2110)
                     /gene="fdhE"
                     /locus_tag="RH222_004048"
     CDS             complement(1181..2110)
                     /gene="fdhE"
                     /locus_tag="RH222_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV3798984.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2107..2742)
                     /gene="fdoI"
                     /locus_tag="RH222_004049"
     CDS             complement(2107..2742)
                     /gene="fdoI"
                     /locus_tag="RH222_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV3798985.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2739..3641)
                     /gene="fdoH"
                     /locus_tag="RH222_004050"
     CDS             complement(2739..3641)
                     /gene="fdoH"
                     /locus_tag="RH222_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV3798986.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNVGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3654..6704)
                     /gene="fdnG"
                     /locus_tag="RH222_004051"
     CDS             complement(3654..6704)
                     /gene="fdnG"
                     /locus_tag="RH222_004051"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6117..6119),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV3798987.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6898..7731
                     /gene="fdhD"
                     /locus_tag="RH222_004052"
     CDS             6898..7731
                     /gene="fdhD"
                     /locus_tag="RH222_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV3798988.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDGALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1779 a         2003 c         2074 g         1892 t
ORIGIN      
        1 gcgataacta aaccattttt gagtaataaa tgaaacaact gcaacactct tattatttct
       61 ggaaaacgcc tcgcaaaaac aaaacaacat gcaaaaatcc gcttttttta aatgattttc
      121 cggcgtaatt ttcgcacata cattaatgag taatataggt aaggtggtgg taatggcgat
      181 gaatacggtt tttcttcatt tatcagaaga ggcaattaaa cggctgaaca agcttcgggg
      241 ctggcgtaag gtttcacgat ctgcaatttt acgcgaagcg gtagagcaat atctggaacg
      301 tcagcaattc ccggtgcgta aagcaaaggg aggcaggcaa aagggggagg tagttggtgt
      361 tgatgatcag tgtaaggagc ataaggaatg acaaaaattt ttcattcttg aatataaaaa
      421 acagatgccc ttattctggt attaatacaa ggctgtttta cttgaactta taataactgc
      481 aactgttaca tcatatctgg aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc
      541 tttctttaaa gattcggcaa gggtactttt ctctccatag attcaaaaat gatattggcg
      601 agatatttat gaactctctg gcaggtatag atatgggcag aattttactc gatttatcga
      661 atgaggtgat taagcaactt gatgatctcg aggtgcagcg taatcttcct cgtgcggacc
      721 tattaaggga agcggtagat caatacctga taaatcaatc gcaaacagca agaaccagtg
      781 ttcctggcat ctggcaaggg tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg
      841 aggaatggta atgggaaaaa atgatgtcaa tcagattgct gacaacgtgc gcgttgttca
      901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaagt cgtataaatt caatgtattg
      961 cagtttattg gtaggcctga taagcgcagc gcatcaggca atcttacgct tgtcatcagt
     1021 ctcatgccca taaccttatt gatgtggtgg tgggtaaatt gaggccaaac cggcactggc
     1081 ggcatagctt gaggtggtaa cgcggaatac gagagatggt gtcgtcacac cctatgagct
     1141 gtaatccggg cgacctgcgc cacccggaaa acgtcagaga ttactctcct tcacccggaa
     1201 acaggaacgg gttgatggaa ctgcgggcat agccttcttg ctccattcgt gcgtccagta
     1261 ccagagaggc gaggtcatct gctacggctt cgatttttgg atctttttcc tgatagagaa
     1321 ttttcaggta agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat
     1381 ccagcgacca gtaatgcagt ttgccgctct gctcgcagtt gctgcatttt acgcgcacta
     1441 cgtgccattc agtttcacac aggttgcagt gcaggtaacg tagcccctga gtggtgccaa
     1501 tttgcaccat gctggacacc ggcatagaac cacaaaccgg gcaatattga cgttgctcgc
     1561 cgtattcagc gcgggctttg ccggggatca gattggccat ctgcgcccag tagagcgaca
     1621 gtgcagccca gataaacggc gctttatcgc tgctgacgga cgagaaatca gaggcaaaca
     1681 gcgcgctggc catatcttcc agctcctgag tcgatgcctt ctccagattc tcaatcactg
     1741 ccagcgccgg gccgctcatt tcaggtttca gctcagcaat cagcgccatc agcagctttt
     1801 gccagtgctt atcacgcggc agaacgtgaa tatccagcgg gggcttgcct tgtgcgctgg
     1861 cttctttaat gcgcgcagtc agatccatct ccagcggatg gtcgtacagc accacttcct
     1921 gggcgtgggc gataagcgca gcaaagcgca ggtaatcacc cagaggatta ttttctgcca
     1981 gctcgcgcag acgctcggcg cggcggttgt ataaattctt gagccgaggg aacaataacg
     2041 gcggaatcat atccgccgta cgtttctcgc ttgaacccag ctcatcttgc gggattatgc
     2101 gaatactcat tcagctttct tttccgttgt cttgcggacc tcacggtacc agcgcgggtg
     2161 atgtttcttc gcccaggcgc tggtaaccca tccttccacc atggcggtaa tcgtgccttt
     2221 cacccaaagg gcggcgtaga tatgcaccat gataaccaca attaacgcca ctgcggcaaa
     2281 tgaatgcagc attaacgcga atcggatcac cgggattgag aaagcaggcg caaaataagg
     2341 acgccagata atcacgccgc tcaccagcag cagaaccagg aaaataatcg ccgcccagaa
     2401 aacgcatttc tgaccgaagt tataacgccc ggtgtcacct acttcctcgt tgacgacgat
     2461 cttacgaata ttcttcgccc aaaagatatc atcccgattg attaggttgt gatgccagta
     2521 acggaaaaac atgatgatga acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat
     2581 tcgcgccagc tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa
     2641 gcccagcccg ctcaccgccg ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg
     2701 ttccggcgcg gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc
     2761 tcttcgtgca gattattctc ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg
     2821 aagatactgg ccgcaaaggt agccgcaaag ccaacagccg cgagcggttt ccagatgcct
     2881 ttccagaatt tcacggtttc gctgatttcc gggttctccg gcaagccatg atacagattt
     2941 ggcttgtcag catggtgcag cacgtacatg acgtgtgtac caccgacgcc tgccggatcg
     3001 tacagacccg cattgtcgta accgcgggtt ttcagctcag caacgcgctc gctcgccagc
     3061 gttttcatcg actctttcgt accgaagtga atcgcgcccg ttgggcaggt cttcacgcag
     3121 gctggttctt gcccaaccac cacgcggtca acgcacagcg tacatttgta gacgcggttg
     3181 tcttccgggt tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata accgcagcca
     3241 atgcactgct cggactggaa gtcgacgata ccgttggcat actgaataat tgcaccttcc
     3301 gccgggcacg ctttcaggca gcctggatcg gaacagtgca tacagccgtc tttgcggatc
     3361 agccattcca gtttgtcgtt ctgctccact tccgagaagc gcattaccgt ccacgatttg
     3421 gcgcttaaat cattggggtt gtcgtacacc ccaacgttat tgccgacggt atcgcgaatg
     3481 tcgttccact ctgaacacgc cacctgacag gctttacagc cgatacaggt ggtgacgtcg
     3541 atgagtttcg ccacttcttc ctggaagtcc cgcgcttgag gcgcgggggt cagaccgtta
     3601 gtcgcggaac gacgaatgat atcttgagat tgataagcca tatgtcgtct ccgttacacc
     3661 ttttccacat tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg
     3721 aatggcgtta acgtattggc aataaagcct tttttagcta cgccttcata gccccagtga
     3781 atcggaatac cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg
     3841 gtcaccaccg ctttggcttt gatatagcca cggttggagg agactttcac ggtatcgccc
     3901 tgggcaatgc caagtttatt cgccagcgac tcaccgattt ccacaaactg ctctggctgc
     3961 aaaatcgcgt tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt cagacgatag
     4021 gtggttccga catacgggaa cttatcggct ttacccaatg cttcggcgtc gtctttaaag
     4081 attcgcgcag ccgggttcga gataacgttt ggatgcagcg ggttagttcc cagcggcgtt
     4141 tcaaacggct cgtagtgttc cgggaacgga ccttccgcca tcttatcgag ggcaaacaga
     4201 cgtcccatgc cttcctgctg catgataaac ggcccgacgc cgctgcccgg aggcgctgcg
     4261 ctgtagtccg gaatatccca gccggtccac ttagtgccgt cccatttcag caactgacgc
     4321 ttcggatccc acgggttacc ctgcggatct gcggaggcgc ggttatacag aatgcggcgg
     4381 ttaagcggcc atgcccatgc ccagcccagc gtgttaccga ggccagacgg atcggcgtta
     4441 tcacggcgtg ccatctggtt gccttccggc gtccagctac cggcgaaaat ccagcaacca
     4501 caggaagttg taccgtcatc gcgcagttgg gcaaacgagc taagttgttg gcctttcttg
     4561 acgataaccg caccggttgc cggatcggta atatcggcca gcgccttacc gttgctctcc
     4621 atcgccactt cttctgaaga tggctcatgc ggaatagcgt agttccaggt catgttcagc
     4681 acctgatccg ggttcgcgcc accctgttcg gcatacatct tgcgcaagcg caggaagata
     4741 ccggagagga tctcaccgtc agtcagcgca atccccgggg cgtccgcacc tttccagtgc
     4801 cactgcaacc agcgacctga gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat
     4861 ggcagacgga acacttcggt ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg
     4921 ttctgccaga agttagaggt ttcagtgttc agcgggtcga tggtgacgag gaacttcagt
     4981 ttcgacagac aaccgatcac tttgtttttg ttcgggaacg aggcaacagg gttaaagccc
     5041 tggcagatat agccattgac cttgccctct ttcatcatct cgaagtattg caggacgtcg
     5101 tagcctttat cccacttcgg caaccagtca aagccccagc tattttccgc cgtcgcttta
     5161 tcaccaaaga aggccttcat catggagacg aagaatttcg ggtagttgcc ccagtagttt
     5221 acctggcctt ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc
     5281 tgcttctcgc ttggcagcgt catgtaacct ggcaggctct gcgacagcag ccccaggtcc
     5341 gtcagcccct gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc tgccatcccc
     5401 atgttgccga gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc accaacggag
     5461 tgttgcgtcc agccgagggc atacaggaac gaggcagttt tatcgtgagc actggtttct
     5521 gcgatgtatt cgcagacttt caggaacgcg tcttttggcg taccacagat gttttcaacc
     5581 acatctggcg tgtagcggga aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt
     5641 tgcagcgtgg tatcgcgttt ggcgaagccg ttttcgtcca gttcataagt ccaggaggat
     5701 ttatcgtact tgcgtttttc cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa
     5761 tcctcacgca cgatcaggct ggcgttggta taggcttcgg tgtattcgcg gttgaatttt
     5821 tcattgttca gcaggtacag caatacgcct gacaggaaag caatgtcagt accggaacga
     5881 ataggggcat agtagtcagc taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc
     5941 agcttcgcgc cgttgtgaat tttggcttcc atcgcccagc ggaacccgac cgggtgagct
     6001 tcagcggcgt taccgcccat caccacgacg aggttggcgt tcttgatgtc gacccagtgg
     6061 ttggtcatcg caccgcgacc aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag
     6121 acacgcgcct ggttgtcgac cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa
     6181 tagccggttt cgttactcga cgcggaagca cacagcatcc cggtggagag ccagcggtta
     6241 acagtcacgc cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt
     6301 ttggcgatgc gatcaaacgc ctcttcccaa ctgatttgct gccatttatc agaacctggc
     6361 gcacggtatt ccggaaactt cagacggctt tcggagtgga tgaaatccac caggccagcg
     6421 cctttcggac aaagtgcacc gcggttgacc gggtgatccg gatcgccttc gatatggaag
     6481 atagatgctt tggcgttttt tgctccgtca ccgaggctgt acatcaacag cccacagcct
     6541 acggaacaat aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt atactgccgg
     6601 gtttccgcga gtgctacgct gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata
     6661 ccgccagcgc agatcttaaa gaactgcctt ctgctgacct gcatggattg ctccttgttt
     6721 cgacattgtc acgtcccatt tacattcgct tgctgcgtgt gcagggagtg ggagttattt
     6781 ttctttgcgg aaggggccgc aaaggtccag aattggctca atttccctcc atccaggaag
     6841 ggtttgtaac agaataccat aatgttggtg tgtgtgttct tatctggtta agagaaagtg
     6901 aaaaaaacac agcgaaaaga aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt
     6961 gagttatggc ggcgtgatga tttacaacac ccacggttag atgaggtcgc ggaagaagtt
     7021 cccgttgcgc tggtctacaa cggcatttcg catgtggtga tgatggcgtc gcccaaagac
     7081 cttgagtact ttgcgctcgg tttttcgctt tccgagggga ttatcgaaag tccgcgcgat
     7141 atcttcggca tggatgtcgt tccttcctgt aatggtcttg aagtacaaat tgagctttcc
     7201 agccgccgct ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt
     7261 ggcgtatgcg gcgtggagca acttaatgac atcggaaaac cggtgcagcc gctaccgttc
     7321 acccagacgt ttgatctcaa caaactggat ggtgcattac gtcatctcaa cgattttcag
     7381 ccagtggggc aactgactgg ttgtactcac gccgctgcct ggatgttgcc atctggcgaa
     7441 ctggtcggcg ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact gttaggccgc
     7501 cggtcacaag aaggggaaag ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt
     7561 tatgaaatgg tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc
     7621 gcgaccacgc ttgctgtaga agtggctgag cgctgtaatc tgacactggt aggtttttgt
     7681 aaaccgggtc gggcaacggt ttatacccat ccgcagcgtt tgagcaatta attaaaataa
     7741 gtcctgcg
//
LOCUS       DAPHJE010000166         7712 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPHJE010000166 DAPHJE010000000
VERSION     DAPHJE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.168"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..371
                     /locus_tag="RH222_004053"
     CDS             <1..371
                     /locus_tag="RH222_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798989.1"
                     /translation="DKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHAR
                     LFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPRY
                     IAEKNYMEALLKKASPHTVR"
     gene            complement(340..2076)
                     /gene="aceK"
                     /locus_tag="RH222_004054"
     CDS             complement(340..2076)
                     /gene="aceK"
                     /locus_tag="RH222_004054"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV3798990.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2259..3563)
                     /gene="aceA"
                     /locus_tag="RH222_004055"
     CDS             complement(2259..3563)
                     /gene="aceA"
                     /locus_tag="RH222_004055"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV3798991.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3593..5194)
                     /gene="aceB"
                     /locus_tag="RH222_004056"
     CDS             complement(3593..5194)
                     /gene="aceB"
                     /locus_tag="RH222_004056"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV3798992.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(5463..6392)
                     /gene="metA"
                     /locus_tag="RH222_004057"
     CDS             complement(5463..6392)
                     /gene="metA"
                     /locus_tag="RH222_004057"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV3798993.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6549..6992
                     /locus_tag="RH222_004058"
     CDS             6549..6992
                     /locus_tag="RH222_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV3798994.1"
                     /translation="MVISIRRSRHEEGEGLVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            complement(7055..7438)
                     /locus_tag="RH222_004059"
     CDS             complement(7055..7438)
                     /locus_tag="RH222_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798995.1"
                     /translation="MSVLYIQISRNKITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLHDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMLKQK"
     gene            complement(7515..7630)
                     /gene="rrf"
                     /locus_tag="RH222_004060"
     rRNA            complement(7515..7630)
                     /gene="rrf"
                     /locus_tag="RH222_004060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1879 a         1967 c         1955 g         1911 t
ORIGIN      
        1 tcgataaaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt
       61 tgttttcagc gatacagcat aaacaacaaa atgttgtaga gacggtttat cttgctttat
      121 ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca
      181 aagcgccaca aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg
      241 ctgaattaat ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc
      301 ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata
      361 ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg
      421 tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc
      481 aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac
      541 atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg
      601 cggcgggatg tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa
      661 aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc caggaaaaat
      721 gttagcagcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg
      781 ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct cgatatagag
      841 atggcgaatt acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag
      901 taattccatt aatgccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg
      961 ggtgtccgcc attcggccca cgcgatcgtg ctctttcacc agttgatagc aggcacgaac
     1021 gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg
     1081 atcaaagccc ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat
     1141 gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc
     1201 gtgcttctgg cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc
     1261 ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc
     1321 aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc
     1381 gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag
     1441 tttgcccacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg
     1501 actttccgcg aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat
     1561 gtcacggctt ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag
     1621 tagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg
     1681 aaagcggcgc tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg
     1741 gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta
     1801 atccggcaac agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact
     1861 ttggccgtta gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag
     1921 atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg
     1981 ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat
     2041 ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag
     2101 aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat
     2161 gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtgaac
     2221 gccttatccg gcctacagtc agcaacggtt gttgttgctt agaactgcga ttcttcagtg
     2281 gagccggtca gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt cactttatcg
     2341 aagtaacctg tacccacttc ctgctggtga gatacgaagg tatagccatc tttcgcggcg
     2401 gcaaattccg gctgctgcac tttctcaacg tagtgcttca taccctcgcc ctgggcatag
     2461 gcgtttgcca ggtcaaacat gttgaaccac atgctgtgga tacctgccag ggtgatgaac
     2521 tggaacttgt agcccatatc cgacagctgc tgctggaagc tggcaatagt tttgtcgtcg
     2581 aggtttttct gccagttgaa cgacggcgag cagttataag ccagcagttt gcccggatat
     2641 ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca gatccggcgt ggaggtttca
     2701 caccagacca ggtcagcata tggcgcatac gccaggccac ggctgatcgc ttgctcaatg
     2761 cccgcatgag tacggaagaa gccttcactg gtacgctcgc cagtaataaa ttcactgtca
     2821 tacgggtcgc aatcggaggt aatcagatcc gccgcatcgg catcggtacg agcaaccagc
     2881 agggttggaa cgcccgtcac gtcagctgca agacgcgccg cgaccagttt ctgaatagct
     2941 tcctgagttg gcaccaaaac tttgccgccc atgtgaccgc atttcttcac tgacgccagc
     3001 tgatcttcga agtgaactgc cgctgcaccg gcttcaatca tcgctttcat cagttcaaag
     3061 gcattcagga caccgccaaa accggcttcc gcatcggcaa cgatcggcag gaagtaatcg
     3121 acatagcgtg gatcgcccgg ctcaatgcct gcggaccatt ggatctgatc ggcacgacgg
     3181 aaggtgttgt tgatccgctc caccacagct ggcaccgagt ttgccggata gagcgactga
     3241 tccggataca tgctggccgc caggttagcg tccgccgcta cctgccatcc cgacagatag
     3301 actgcttcaa tacccgcttt cgcctgttgc agcgcctgac cgccagtcag tgcaccgagg
     3361 ctgttgatgt agcctttttt cgactcgcca tgcagcagac gccacatttt cgccgcgccc
     3421 agttgcgcca gggtgcattc aggattgact gaaccgcgta atttcaccac atcttccgcg
     3481 ctgtatgggc gagtaatgcc ttcccaacgc ggttgagtcc actctttctg taattcttca
     3541 atttgttgtg tacgggtttt catgtgcaga tgctccatag ttatgtggtg gtttacgcta
     3601 acaggcggta gcctggcagg gtcaggaaat caattaactc atcggaagtg gtgatctgtt
     3661 ccatcaagcg tgcggcatcg tcaaaacgcc cctgggagaa acgttcttcg cccagttcgc
     3721 tggcaatgac tttcatctct tcacccagca tctggcggaa caaggctttg gtcaccggtt
     3781 tgccattgct caacgttttt tgatgatgga tccactgcca gattgaggta cgggaaattt
     3841 cagccgtcgc cgcatcttcc atcaggccat aaatcggcac gcagccgttg ccggagatcc
     3901 atgcttcgat gtactgcact gccacgcgaa tgttggcgcg cataccttct tcggtgcgtt
     3961 caccatcaca aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcatca
     4021 cttcaagctg atttttacgg gagccgagaa tgtcgttgaa taccgccatt gccgtatccg
     4081 caaggcctgg gtgagcaatc catgtgccat cgtgaccgtt attggcttcc agcgctttat
     4141 ccgcttttac tttgttgagc acctggttat tgcgctcttc atctttgctc ggaataaacg
     4201 ctgccatgcc gcccatcgca aaagcaccgc gtttatggca ggttttaatc aacaggcgtg
     4261 agtaagcatt caggaatggt ttatccatcg tcactgcctg tctgtctggc aggacgcgat
     4321 cgggatagtt tttcaacgtt ttgatatagc tgaagatgta atcccaacga ccgcagttca
     4381 gaccaacaat atggtcacgc agcgcgtgaa ggatttcatc catctggaac acggcgggca
     4441 gcgtttcaat cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag cgatcttctg
     4501 catagctgaa gacttcgctc caccaggccg cttcctgcca ggactgggtt ttcggcagat
     4561 agaaataggg accgctgccc tttgccaaca gtgcctgata gttgtggaag aaatagagcg
     4621 caaaatcaaa caggctaccg gggattgcct caccacgcca ggtgacatgt ttttccggca
     4681 agtgcagacc gcgtacccga caaatcaaaa ccgctggatt gggcttgagc tgataaattt
     4741 tgcctgcttc attggtatag ctgatggtgc cgttaaccgc atcgcgcagg ttaatttgcc
     4801 cgtcgatcac tttgttccag tctggtgcca gtgaatcttc gaaatcggcc ataaagactt
     4861 tcacattggc gttcagtgcg ttgatcacca tcttgcgctc aaccgggcca gttatctcga
     4921 ctcgacgatc ttgtaagtcc gcgggaatac cacgaatttt ccagtccgta tcgcgaatgg
     4981 aagctgtttc cgaaataaaa tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa
     5041 tgcgcgctgc cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa
     5101 attctaccgc ttcggcagta agaatttgct tctcctgctc gccatacggc cttatgaaag
     5161 ccagttcatc ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc atggatccga
     5221 aaacttcccc actgtgaacg aaggatcgtt gtgcgctttt cgttcagcac aactaagact
     5281 actcatttaa aatgcaaaat caaaaacaat ttccatttta attaaaaata cttgataact
     5341 tattgataac aatatgaata aaatttattc acctcctgag gtgcgtttcg gaaataaaaa
     5401 aggcacccga aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa
     5461 gattaatcca gcgttggatt catgtgccgt agatcgtatg gcgtgatctg atagacgtaa
     5521 tagttgagcc agttggtaaa cagcaaatta ccgtgactac gccagctcgc tcgcggtgta
     5581 ttttgcggat cattgtgcgg gaaatagtta tacggtactt ccgggcctag tccggcttcc
     5641 acatcgcgga aatattcctg cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca
     5701 aaggcaatgc gcttatcttt gctggcaaac agatatgcat ccccttcttc cgtctctgcc
     5761 agaatttcca gatcggtgta atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa
     5821 tgtggtgcca ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg atggagaata
     5881 tgatgctcgt aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg
     5941 atattgagcg cggcctgtac cgcccagcag acaaacagcg tcgaggtgac gtgatctttc
     6001 gaccactcca gcacctgttt gatctgcggc cagtaagcga catcattaaa ctccaccagg
     6061 cccagcggcg caccggttac aatcagaccg tcaaagttct gttcctgaat atcttcaaag
     6121 ttacagtaga agttgttcag atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg
     6181 cgcaacagct gaatatcgac ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt
     6241 tcagtttcaa tcttcttcgg catcaggtta aggatcagaa ccttaagtgg acgaatttcc
     6301 tgaccagacg cacgagaagt tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg
     6361 gcgggtagct cgtccggcac acgaataggc ataacctgat tacctcacta catacgctta
     6421 tacgtttaga catccagata gctgaagata accagaaaat ttgccaatgt cgagcctgca
     6481 tgttgaaggt gagaaagttt caaggaaaag gctacgttag aatataagaa tgacgaaaag
     6541 gagagaaaat ggttattagt attcgccgct cacgacatga ggaaggggag ggactcgttg
     6601 cgatttggtg tcgatctgtc gatgccactc acgatttttt atcagcagag tatcgggccg
     6661 agctggagga gctggttcgt tcctttctgc cggaagcacc gttgtgggtc gcggttaatg
     6721 agcgggaaca gccggttggg tttatgttgt taagtgggca gcatatggat gcgctgttta
     6781 tcgatcctga tgtgcgcggc tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga
     6841 tggcaccgga actgacaacc aacgttaatg agcaaaatga gcaggcggtt gggttctata
     6901 agaaggtggg ttttaaggtt acgggacgct ctgaggtgga cgatttgggg aaaccgtatc
     6961 cattgctgaa tctggcatat gtcggggcgt aggcgttttt ttctcatccc gctgggctga
     7021 acttcaggtt tcgtacccac caatggtgac acgattattt ctgcttaagc attgccatga
     7081 ccgcactatc actgagtacc actgattgcg catggatgtg gaactggcga tatttcatta
     7141 acgttgcccc agcgaccact tcatgaagaa tacgttcctc aacctgtgac aaaccgccct
     7201 cattcatttc cagcgcgcta acaacaatat ccatacgttt tgctggcaaa aaagaatgcc
     7261 aggttgaacg tgggtggagt tgcagaacaa gatcatgcag aactttttct gcaacaaaga
     7321 aattggcgat taacaggcgc tggttactga aggcagcgtc tcctgacact tcacgtttgc
     7381 tttcaagatc gcgaacagta attttattac gactaatttg gatatacaga actgacatga
     7441 gattcccttc atcatgcaaa taattgatat gcaaaaaacc ccggccttat ggaccggggt
     7501 ttttctaatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
     7561 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
     7621 ggccgccagg caaattctgt tttattaacc cgcttatcgc cggttaatct aatctgtatc
     7681 aggctgaaaa tcttctctca tccgccaaaa ca
//
LOCUS       DAPHJE010000167         7698 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPHJE010000167 DAPHJE010000000
VERSION     DAPHJE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.169"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..558)
                     /locus_tag="RH222_004061"
     CDS             complement(<1..558)
                     /locus_tag="RH222_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3798996.1"
                     /translation="MFEAYLGNPKRNIKNAHILEKYQADIDALSTMFSEDDLLSLMTQ
                     AGPGNAVFMKSAREVLYVLPSSGVNSNQINFFQDTSYWYKWRELQQQEFHRAAVLIFK
                     SSSRKNKIPQAIIEQLDEFILQEIGNAGRCKKGILSLFLHSFLDNMYHSEKFMNPVEN
                     YQNGVATFRHFQRTLTRYNTYVTNHL"
     gene            867..2390
                     /locus_tag="RH222_004062"
     CDS             867..2390
                     /locus_tag="RH222_004062"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDV3798997.1"
                     /translation="MNDKISQDTINKALWAACDTFRGTISADTYKDFILTMLFLKYIS
                     DVWQDHYDEYKEQYGDAPELIEAMMANERFVLPKSASFYALYERRHEPGNGERIDQAL
                     HAIEEANGTKLKDAGKSVFQDISFNTDRLGEEKQKNTILRQLLEDFAGEDLNLKPSRV
                     GTLDVIGNAYEYLIKNFAASGGQKAGEFYTPPEVSDLIAELLDPQPGDTICDPACGSG
                     SLLMKCGRKIVSGHDSRNYALFGQEAIGSTWSLAKMNMFLHGEDNHKIEWGDTIRNPK
                     LLDKNGDLMLFDIVTANPPFSLDKWGHDEAENDKFGRFRRGVPPKTKGDYAFISHMIE
                     TLKPGTGRMGVVVPHGVLFRGSSEGKIRQKLIDENLLDAVIGLPEKLFYGTGIPAAIL
                     IFKKQKVDDKVLFIDASREFKAGKNQNQLSEENIEKIVKTYRDGDNVEKYAYLASLKE
                     IQDNDYNLNIPRYVDTFEEEDEIDIRAVHAEREQLKVELAKLETEMAGYLKELGYGA"
     gene            2380..3606
                     /locus_tag="RH222_004063"
     CDS             2380..3606
                     /locus_tag="RH222_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV3798998.1"
                     /translation="MVPKGWSSWILSDICRKQISYGIVQTGDNLPNGIPCLRVVDLTR
                     DVMRLEDMIKTSEETNKAYRKTILEKDEIVMALRGEIGLARLIDDNLVGANITRGLAR
                     ISPETKVVLPEFLLWELRSPQFRADLIRRVGGSALQEISLTELRKVRTLLPPLLEQKK
                     IAQILSTWDKAISVTEKLLVNSQRQKKALMQQLLTGNKRLLDENGVRFSGEWQSLQIS
                     DIGTVVTGSTPPKNDSDNYGGDISWATAEDFVGKYVSNTKIKLSEQGAKKARIVPKGS
                     ILVTCIASIGKNAIADENMGTNQQINSVIVRGSNSNEFFYYQIEFNVKTLLTLAGTTA
                     VPIINKSSFEKVSLKFPHFEEQQKIADVLSAADAEITTLQKKLACLKEEKKALMQQLL
                     TGKRRVKVEAEEAVSA"
     gene            3628..6912
                     /locus_tag="RH222_004064"
     CDS             3628..6912
                     /locus_tag="RH222_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDV3798999.1"
                     /translation="MNQPYTPKFQEEYSAKIPALTLLTSLGWTFLPPKQIMDYRGYKQ
                     DEVVLRPVLREELSKRSFMAGGKTCQLSEKALDNLISQVCSPALNEGLLKANERMYNH
                     LLYGIAVTEFVDGKKVNPTIALIDWEHPENNQFHFTEEFTVLRSGGVETRRPDIVCFV
                     NGIPLAVIEAKSPAGHGKKGPTIDEGISQSIRNQLNDEIPQLFAYSQILLSINGHDGR
                     YGTCHTPMKFWAAWREEDITDAQMYALRNHPLSTEQIHALFDHRPSADLNWYQQLIAA
                     GELAVSGQDKLLISLLSPERLLEMTRFFTLFDKKTGKIVARYQQVFGIKRLLERISTR
                     RPDGGREGGVIWHTTGSGKSYTMVFLSKALILHDSLKQCRIVVVTDRVDLEGQLSGTF
                     ASGGELAGKDDKAKAMATSGQKLAQLIGSGKERIIFTLIQKFNSATKLPECVNTSPDI
                     IVLIDEGHRSQGGENHVRMKLALPNAAFVAFTGTPLLKEDKTTNKFGPIVHAYTMQRA
                     VEDKAVTPLLYEERIPDLEVNDRAIDAWFDRITDGLSEAQKADLKRKYARKGEVYSAD
                     DRIRLIALDIATHFSKNIDEGLKGQLACDSKISAIKYKKYLDEAGLFESAVVISPPDT
                     REGNTEVDESKLPEVTKWWKDNVGTQDESVYTRNIISRFDTDEKLKLLIVVDKLLTGF
                     DEPKNTVLYIDKPLKSHNLIQAIARVNRLHPLKKFGLLIDYRGILAELDTTIGKYQDL
                     ASRTQGGYDIKDIDGLYSAMSSEYKRLPHLYNQLWAIFAGVKNKNDTEQLRAVLVPKM
                     EERDGEMVDIHQKTRDDFFEALTAFAGCLKVALQSATFFTDKSFTEQDRNLYKETVKQ
                     MSSLRQWAMQVSGEQVNYDDYAEQVKKLLDKHVTGVEVREPDGVYEVGKMGKSEKPEE
                     WDNNKTRNETDIIKTRVTKMIEQELRDDPYAQEAFSRLLRMAIEEAEKLFDHPLKQYL
                     LFREFEEQVEARKLSDIPDALAVNKHAQAYYGVFKKELPEVFAVNDVQVQDKWTKLAF
                     EVDNIIVKAVAENSLNPQDIEKVVKTSLLPLLFTACREIGAGMNQVNRIVETIIQILR
                     VGLMKS"
     gene            6909..7673
                     /locus_tag="RH222_004065"
     CDS             6909..7673
                     /locus_tag="RH222_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV3799000.1"
                     /translation="MSEKESPSMKALRIVYGDEVIVVQCFPRQVVKGRVLIKVHPDCR
                     VIVSVPPETPEHEVLSALKKRSRWIYQQLRDFREQQTHIVPRQYVSGESHYYLGKQYQ
                     LKVTEDATVPQRVKMLRGRLEVTVRHKSAEKVKALLAEWYRERARDVFQRRLDLLIPQ
                     TLWVSERPPIRLRAMQTQWGNCSAKGCLTLNPWLVKASSECIDYVLLHELCHVAEHNH
                     SEEFYRLMGQVMPGWEKVKKRLDGMAGMLLADVIEN"
BASE COUNT         2184 a         1655 c         2005 g         1854 t
ORIGIN      
        1 gagatgattg gttacatacg tattgtaacg cgttagcgtt cgttggaagt gtctgaaagt
       61 tgctacccca ttttgatagt tttcaaccgg gttcatgaat ttttcagagt gatacatatt
      121 atctaaaaaa gagtgtagaa aaagagatag aatgcctttc ttacagcgtc cggcgttacc
      181 aatttcttgt agaataaatt catcaagttg ttcaattata gcttgaggta ttttgttttt
      241 tcgggatgaa ctcttaaaaa taagtactgc agccctgtgg aactcctgct gttgcaattc
      301 ccgccattta taccaatacg aagtgtcctg gaagaaattg atttgattag aattaacacc
      361 acttgaggga agaacataaa gtacctcacg cgcagatttc ataaacactg catttccagg
      421 gccagcctga gtcatcagac taagcaaatc atcctcagaa aacattgtgg ataacgcgtc
      481 aatgtctgct tgatattttt cgaggatatg agcgttttta atgtttcgct tcggattgcc
      541 aaggtaagct tcaaacatag ttatgccctt ttaacattta aagccacatg agaggcttta
      601 tgtagtgata gcgggatata tcattacccc aacggcagtt ttacaccata gctaatatgg
      661 atgttatctt actaagataa ttggtttttt gcgagtggcg aaacaggttg tgtcagttta
      721 tggcatgtcg atatgatggg gacgatgcca accggaatgc tgcgatattg gcgtgtcagg
      781 agaggcatct tacggtatca tttaccgcaa ttttttacga tgcaaagccc tgcatcgcta
      841 gctttttctt cagaccagga tgattcatga acgacaaaat cagtcaggat acgatcaaca
      901 aagccctgtg ggccgcgtgt gatacgttcc gtggcaccat cagtgctgat acctataaag
      961 actttatcct tacaatgctg ttccttaagt acatttcaga tgtctggcag gatcactatg
     1021 acgaatataa agagcaatat ggtgatgcgc cggaactgat tgaagcgatg atggcgaatg
     1081 aacgtttcgt gttgccgaaa agcgccagct tttatgcgct gtatgagcgc cgtcatgagc
     1141 caggcaacgg tgaacgtatc gatcaggcat tgcatgcgat tgaagaagcc aatggcacca
     1201 aactgaaaga cgccgggaaa agtgtcttcc aggatatttc gtttaacact gaccgcttgg
     1261 gtgaagaaaa acagaagaac accatcctgc gtcagctgct ggaagacttt gccggtgaag
     1321 atcttaacct gaagccaagc cgtgtaggta cgctggacgt tatcggtaat gcctacgaat
     1381 atctgattaa aaactttgcc gccagcggtg ggcaaaaagc cggggaattc tataccccgc
     1441 cggaagtctc ggacctgatt gctgaactgc tcgatccgca gccgggtgat accatttgtg
     1501 atcctgcctg tggttcaggt tcgttgttaa tgaagtgcgg gcgtaagatt gtctccgggc
     1561 atgacagccg taactatgct ttgttcggtc aggaagcgat tggctccacg tggtcgctgg
     1621 cgaaaatgaa catgttcctg catggcgaag acaaccacaa aatcgagtgg ggcgatacca
     1681 ttcgtaaccc gaagctgctc gataaaaatg gcgacctgat gctgtttgat atcgtgaccg
     1741 caaacccacc gttcagcctg gataagtggg gtcatgacga ggccgagaat gataagtttg
     1801 gtcgtttccg tcgcggtgta ccgccgaaga ctaaaggaga ttacgccttt atctcacaca
     1861 tgatagaaac cctaaaaccg gggactggtc gtatgggcgt ggtggtgcca cacggtgtgc
     1921 tgttccgtgg ctccagcgaa ggcaaaatta ggcagaaact gattgatgaa aatctgctgg
     1981 atgcggtgat tggtctacca gagaaattgt tctacggtac ggggattccg gctgcgattc
     2041 tgattttcaa aaagcagaaa gtggatgaca aggtgctgtt tatcgatgcc agccgcgaat
     2101 tcaaggcagg taaaaaccag aatcagctca gcgaagaaaa cattgagaaa atcgtcaaga
     2161 cctaccgtga tggcgataac gtcgagaaat acgcctatct cgccagcctg aaggagattc
     2221 aggacaacga ctataacctc aatatccctc gttatgtcga tacctttgag gaagaggacg
     2281 agattgatat acgtgccgta catgccgaac gtgagcaatt gaaagtggaa ctggcgaagc
     2341 tggaaacgga gatggcaggt tacctgaagg agttgggtta tggtgcctaa gggatggagc
     2401 agctggatat taagtgatat ttgtagaaag caaatatcat atgggattgt acagacaggg
     2461 gataatttgc ccaacggtat accatgtctt cgagttgtgg atctcacacg agatgtcatg
     2521 cgtctcgagg atatgattaa gacatctgaa gagacaaata aagcatacag aaaaacaata
     2581 ctggagaaag atgaaatcgt aatggcattg cgtggagaga ttggcttagc acgtcttatt
     2641 gacgataatc ttgtcggcgc aaacattact agaggtttgg caaggatctc tcctgaaact
     2701 aaagttgttc ttcccgagtt cttactgtgg gaacttcggt cccctcaatt ccgggcggat
     2761 ttaatacgga gagtcggtgg atcagcatta caagaaatat cgctgacaga attacgcaag
     2821 gtaagaacat tattaccccc ccttcttgaa cagaaaaaaa tagcccaaat cctatcaacc
     2881 tgggataagg cgatttcggt gacggaaaag ctgctcgtca atagtcagcg gcagaaaaaa
     2941 gcgctgatgc agcaattgct taccgggaat aaacggttgc tggatgagaa tggggtgagg
     3001 tttagtgggg agtggcagag tttacaaatt agtgacattg gaacggttgt cacgggctct
     3061 acacccccga agaacgacag tgataactat ggtggggata tatcatgggc tactgcagaa
     3121 gattttgttg ggaaatatgt ttcaaacaca aaaattaagc tgtccgaaca gggagctaaa
     3181 aaagccagaa ttgttccgaa gggtagtata ttagttacat gtatagcctc aatcgggaag
     3241 aatgctattg ctgatgaaaa tatgggcaca aaccagcaaa taaactcagt tattgttcgt
     3301 ggttccaatt caaatgaatt tttctattat caaatcgaat ttaatgtaaa aaccctcctc
     3361 acgttagcgg gaactactgc tgttcctata ataaataaaa gcagttttga aaaggtttcg
     3421 ctaaagtttc ctcattttga agaacaacaa aaaatcgctg acgtcctgtc cgcagccgac
     3481 gccgaaatca ccacgctgca gaaaaagctc gcctgtctga aggaagagaa aaaagccctg
     3541 atgcagcagt tactgaccgg gaaacgtcgt gtcaaagtag aagctgaaga agccgtgagc
     3601 gcttaacgcg aagaataagg acagctaatg aaccagccgt acacgccaaa gtttcaggaa
     3661 gaatacagcg ccaaaatccc ggcactgacg ttactgacca gcctcggttg gacgttttta
     3721 cccccaaaac agattatgga ttaccggggc tataaacagg atgaggtggt gctgcgtccg
     3781 gttctgcgtg aagagctgtc gaagcgttcc tttatggcgg gtggcaaaac ctgccagtta
     3841 tcggaaaaag cactggataa cctgatatca caggtctgtt ctccagcgct gaatgaaggg
     3901 ctgctcaaag ccaatgaacg gatgtataac catctgctct acggtatcgc cgtcacggag
     3961 tttgtcgatg gcaagaaggt caaccccacc attgcgctga tcgactggga acatccggaa
     4021 aataaccagt tccattttac cgaagaattt acggtattgc ggtcaggcgg tgtcgaaacc
     4081 cgcagaccgg atattgtgtg ttttgtgaat ggtatcccgc tggccgtcat tgaagcgaaa
     4141 agcccggcgg gtcacggcaa gaaagggcca accattgacg aagggatatc ccagagtatc
     4201 cgcaatcagc tcaacgacga aatcccgcag ctgtttgctt acagccagat attactgtcg
     4261 attaacggtc atgacgggcg ctacggtaca tgccatacgc caatgaaatt ctgggcggca
     4321 tggcgcgaag aagatattac cgatgcgcag atgtatgctc tgcgcaacca tcctttatcg
     4381 actgaacaaa ttcatgcgtt gtttgatcat cgtccatcag ccgatctcaa ctggtaccaa
     4441 cagttaatcg ccgccggtga actggctgtc agtggacagg acaaactgct catcagcctg
     4501 ctttcgccgg aacgtctgct ggagatgacc cgcttcttca ctctgtttga taaaaagacc
     4561 gggaaaattg tcgcccgcta tcagcaggtg ttcggtatta aaagactgct ggagcgcatc
     4621 agtacccgca gacccgatgg gggaagggaa ggtggtgtta tctggcatac caccggctct
     4681 ggcaaatcgt acaccatggt gttcctcagc aaggcgctga tccttcatga cagcctgaag
     4741 cagtgtcgca ttgtggtggt gacggaccgt gtcgatcttg aagggcaact cagcggtact
     4801 ttcgcttctg gtggagaact ggccgggaag gatgataagg ccaaagcgat ggcgacctca
     4861 ggtcagaagc tggcacagct gattggctct ggtaaagaac gtataatctt tacccttatc
     4921 cagaaattta actcagcgac aaaattgcct gaatgcgtca ataccagccc cgacatcatc
     4981 gttctgattg atgaaggaca ccggagccag gggggggaga accatgtgcg aatgaaactg
     5041 gccctgccga atgccgcctt tgtggcattt actggtacgc cattgctaaa agaagacaag
     5101 accaccaata aattcggacc gattgtccac gcttatacca tgcagcgagc ggtagaagat
     5161 aaagccgtta cacctttgct gtatgaagaa cgtattcccg accttgaggt taacgatcgg
     5221 gcaatagatg cctggtttga ccgtattacc gatgggttaa gcgaagcgca aaaagccgac
     5281 cttaaacgta aatatgcccg taaaggtgag gtctacagtg cagatgaccg catccggctg
     5341 atcgcactcg atatcgccac acacttctcg aagaatattg atgaggggct gaaaggccag
     5401 cttgcctgcg acagcaagat ttccgccatc aaatataaaa agtatcttga tgaagccggg
     5461 ttgtttgagt cagctgtagt tatcagcccg ccggataccc gggaagggaa tactgaggtg
     5521 gatgaaagca aactgccgga agtgacgaaa tggtggaaag ataacgtcgg cacgcaggat
     5581 gagtctgtgt atacccgcaa tatcatcagt cgtttcgata ccgatgagaa gctcaaattg
     5641 ctgatagtcg ttgataaact gctcaccggg tttgatgagc cgaagaatac cgtgctgtat
     5701 atcgacaagc cgcttaagtc ccacaacctt atccaggcga ttgcgcgggt gaaccgactg
     5761 catccactga aaaagttcgg tctgctgatt gattatcgcg gtattctggc tgagctggac
     5821 acgactatcg gcaaatatca ggacctcgcc tcccgaactc agggcggata cgatatcaaa
     5881 gatattgacg ggctgtacag cgccatgagc tctgaataca agcgtctacc gcatctgtat
     5941 aaccagctgt gggccatatt tgccggtgtt aaaaacaaaa atgatactga gcagttacgt
     6001 gcggtactgg tgcctaaaat ggaagaacgt gacggagaga tggtcgatat ccatcagaaa
     6061 acccgtgatg atttcttcga ggcgttaacg gcctttgccg gatgcctgaa agtggcactg
     6121 cagtccgcta ccttcttcac cgacaagagc tttacggaac aagaccgaaa tctctataaa
     6181 gaaaccgtaa aacagatgtc cagcctgcgc cagtgggcga tgcaggtcag tggagagcag
     6241 gtcaattatg acgactacgc cgagcaggtg aaaaaactgc tggataagca tgtcactggc
     6301 gttgaggttc gtgaaccgga tggcgtgtat gaagtcggta aaatgggtaa gagcgaaaag
     6361 cccgaagagt gggacaacaa taaaacccgc aatgaaaccg acatcattaa aacccgtgtg
     6421 acgaagatga ttgagcagga gctgcgtgat gatccgtatg ctcaggaggc cttttccaga
     6481 ctcctgcgta tggcaatcga agaggctgaa aaactgttcg accatccact taagcagtac
     6541 ctcttattcc gtgagtttga agaacaggtt gaagcccgta agctcagcga tatcccggat
     6601 gcactggcgg taaacaagca tgctcaggcg tattacggtg tatttaaaaa agagctacca
     6661 gaggtttttg cggtaaacga cgttcaggtg caggataagt ggacgaagct ggcttttgaa
     6721 gtggacaaca ttatcgtcaa agcggtggca gaaaactccc tgaatcccca ggacattgag
     6781 aaagtcgtta agacaagtct gctaccactg ctgtttacgg cctgccggga aattggtgcc
     6841 ggaatgaatc aggttaaccg tattgtggaa accatcatcc agatactgcg cgttggtttg
     6901 atgaaatcat gagtgaaaaa gaatcgccat caatgaaagc gttacgcatt gtctatggtg
     6961 atgaggtcat cgtcgtacag tgttttccgc gtcaggtcgt aaagggcagg gtgctcatca
     7021 aggtgcatcc ggactgccgg gttattgtct ctgtacctcc agaaacaccg gagcacgagg
     7081 tattgtctgc gctgaaaaaa agaagtcgct ggatatacca gcaactgcgt gatttcaggg
     7141 agcaacagac acacattgtt ccgcgtcagt atgtcagcgg agaaagccat tattatctcg
     7201 gtaagcaata tcagttgaaa gttacagaag acgctactgt accgcaacgc gtgaaaatgt
     7261 tgcgtgggcg tctggaagtg actgtcagac acaaatcggc agaaaaggta aaagcgttgc
     7321 tggccgaatg gtatcgggaa cgtgccagag atgtatttca acgcaggctt gatttattga
     7381 taccccagac cctttgggtc agtgagcgtc ctccgatacg tctgcgggcg atgcaaacgc
     7441 aatggggcaa ctgctctgca aaaggctgcc tgaccctgaa tccatggctg gttaaggcat
     7501 cctcagaatg tatcgattat gtcttgctac atgagttatg tcatgttgct gagcacaacc
     7561 atagtgagga gttttaccgc ctgatggggc aggttatgcc ggggtgggaa aaggtcaaga
     7621 agcgactgga tggtatggct gggatgttgt tggctgacgt aattgaaaat taactagttg
     7681 tttgatatgt aacgtttt
//
LOCUS       DAPHJE010000168         7668 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPHJE010000168 DAPHJE010000000
VERSION     DAPHJE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.170"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..255)
                     /locus_tag="RH222_004066"
     CDS             complement(<1..255)
                     /locus_tag="RH222_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799001.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFR"
     gene            complement(267..4520)
                     /locus_tag="RH222_004067"
     CDS             complement(267..4520)
                     /locus_tag="RH222_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3799002.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYG
                     ESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLTAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATISPDGKRSTI
                     EYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TCEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLTG
                     QLQSRHLNLPQLDCDYTWNDNGQLARISGPQECREYRYSGTGRLTGVHTTAANLDIDI
                     PYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVI
                     RMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVN
                     GDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSRA
                     SLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITI
                     IESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            complement(4596..6737)
                     /locus_tag="RH222_004068"
     CDS             complement(4596..6737)
                     /locus_tag="RH222_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV3799003.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(6947..7465)
                     /locus_tag="RH222_004069"
     CDS             complement(6947..7465)
                     /locus_tag="RH222_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV3799004.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT         1492 a         2429 c         1907 g         1840 t
ORIGIN      
        1 tctgaaatac ttcatttgca ataaatcaat tatcttacca gcataaagat cgctaatttc
       61 attcaccacc actttatatc tgtatatttc attaagacct tccttgctaa gcggaactaa
      121 cgtggtaaca tacgatggaa gattaattat ctctatcgtt ttcacgctat ctattccaca
      181 caattcattc actcgcccca tccccagtac cgaaccagaa aatagagaca ataaaaccat
      241 caaatatttc atcataaaaa acctcactat tctggcgtac ccgttatttt catcgtctgc
      301 aacggtccat ccacccgata aaaaggcagt ccactatgag ctcctctggt acactcacct
      361 aaagttttcg ccgcacgcga ttcaattatt gtaataaccc ttgcctcttc tttattctca
      421 tatttcttat ctgttcggct taaaagagcc atgaaatttt ccttatcaag aaggtaatac
      481 tgcgcatgag caaactcatg tcccaaacct aatgcaggcg attgtgattt cgagtttaat
      541 tcagttgaac aaaataaaga tgctctacta ttccaatata tcgccatatt attcgaatta
      601 aatctaacat tggttccttc aatatactct atatttatag tttcttcaga gcttgataaa
      661 aaatctatag tttctttcat ttgtgaatct tgttttaaat actcaacagc ctgattaaaa
      721 tcacttggat caccgttaac atggaatttt aaacccaaag aatctataaa ccgaacaggg
      781 tttaatggat atttatacag attccattca cctctcagcc ctatcgggtc ctgggtgata
      841 tatctcccct gcaacggatc atagtaccgg ttgcggttgt agtacagccc cgactcctca
      901 tcatactgct gtcccggcag acggagtgac tgctgaaggt gctgcgcgct ttcctcgccc
      961 agcaggtttc cccactcgtc atactccccc tgccacgccg tttccccttc cgggctgata
     1021 agcgccagcg gcagcccacg gtggtcacag tggtaaagat gaagtttcct ctccgggatg
     1081 tattccgcct ccagttgcgc ggccatctgt tccgccgtca gcccgcactg cgcaagccac
     1141 tgctggcttt cttcactcac gctgccctgc cgcagttccc gctccagcct ccccagcatc
     1201 accgccagct ccgccggtag cgtcacaccc gtgtcctcct gcaacacctc cgccaggcta
     1261 cggtgccgcg ccttcgcctg ttcaccattc tctgtttcga ttctcaggag cggcgtgaag
     1321 cttcccggct ggtataccgt ctggattctt gtctgttggg tctgtacagt ggtcagcctg
     1381 tcaccgtccc acccgtacca ggtctcctcc ggtttacgcg acagcgacat ccaccccgtc
     1441 aggtcacgct cccgccgcca cacccgtttt cccgtccggc ggcccagcgg gtcatagagg
     1501 tagcggctct ccacctgcgg ctcaccgtgc tgtatccgcg tgtagaacac cagacggtgc
     1561 tggctgtcgt agtggtagtg gtgggtccgc tcgtcgtgca tccgtatcac cccttccggg
     1621 atgcggtccg tcttctccgc cagcctgccg tattcatcgt agcggtagac atagtgcgca
     1681 tcttccgcga tgcggttatc cggccacgcc gtgagggtgc tgtccgggtg cagttccggg
     1741 tccggcagcc ggtttcctgc cgggtccgtc gcatacggga tatcgatatc caggttcgct
     1801 gccgtagtgt ggacgcccgt cagccttccc gtgccgctgt aacggtactc ccggcactcc
     1861 tgcgggccgc tgatgcgcgc cagttgtccg ttatcgttcc acgtatagtc acagtcaagc
     1921 tgcggaaggt tcaggtgcct gctctgtagc tgccctgtga gggtgtacgc cgtggcctgt
     1981 tcgtaccccg ctgtactgcc cgccccgccg aagctacggg ccgtctcgcg gtgcagccgg
     2041 tcccgcgtgt actcgaccag cggcgttccg cccagcttca tccccgcaag ataaccgctg
     2101 ccatacgtca gccactccac cggcggcaga ccgtccggct cctgacgggt cgccagcccc
     2161 tgttcgctgt acgcatgccc cgtctcatgc tcccacagca tctcccctgt ctccgggttc
     2221 tccaccgtct gccgttcacc cgtcaggcgg cctttatcgt catagccgta gtggaccgac
     2281 acgcggtggc cttcacaggt atggctgagg gtggttaacc acccgtgctc atcatactgc
     2341 cactgctctg ccgggtcgcc gttcaccgtc cggtgcgtga tgcggtccga cgcatcgtag
     2401 tgccagaggg tgataagccc ctcgtcttca ctctgcgtga gttttccggt caggtcatag
     2461 tggtaacgtt gcgtccggcc gtcaaaaccg cgctgttcag tcagcctgtc caccgggtca
     2521 taccggaacg tggactggct gccgttctcg ttggtcagca cggtgatgcg cccggcagcg
     2581 tcatacccca tgctgcgcgt aagaccgcct tccgtcacac tcaccggacg cccgcgttta
     2641 tcatattcga tggtgctgcg tttaccgtcc ggtgatatcg ttgctgtcag gtcgcctgcg
     2701 gcgctgtact cataacggat ttcacgcccc tgcgcatcct tctgactgac cagttgccca
     2761 cgcgggttat aactgctgta agtgctgatg ccttcctccc ggtgaacggc gatttgttga
     2821 ccgtaccggt cgtactcata ccgcgtcgtg taccccgagc agtccgtgaa ggccagcagt
     2881 tgaccgtaac ggctccatgc catctgtttt gtactgcccg tcgcgtcctg tatccctgtc
     2941 ggcagctcac tcgccgggtc atcatagctg taccgcgttg tctcaccgct gcgcgaggtc
     3001 tccgccgtca gccttccctt ctcatcatac tcccggctgc tgcgaagccc gtccgggtac
     3061 gtcaccgacg tcacctgccg ctggctgtta tagccatacc gtaccgtcct gccgtccggc
     3121 cccgtcaccg ccgtcaccgc acccgacgcc atatgcaggc tgtactccgt ccgccgcccc
     3181 gccgcatccg tctgtgcctt cagcctcccc gcctcatcat actcgctgcg ggtaatgctc
     3241 ccgtccgcat gttccttctt caccacccgc ttcaggccac cctcgccttc cgtgtacagc
     3301 acctcccgcc ggttcaggct gtccgtgatg attacccggc tctctccgta ctcaaagcgg
     3361 tagtccagcc cctccgggtt gacctgctcc gtcacccgac cggtatcatc ataccggtag
     3421 cagctctccg gcctccccgc ataatggtgc gccaccatcc gcccggcgtg ctccgcatca
     3481 taagtaaacc cgcgcacctg cgttccgctg cggtcataca ccgcccgcag ttcaccaccg
     3541 gccgtgtacg tgtagcgcgc cagcggcgca gtcggctgtt catccgggta tgccgggtcg
     3601 tgtgtcagcc ataccgcctc cagccggata ccgttatccg tgccgtatcc tgtaccggcg
     3661 ggcagcgtgt cggggaaaac cagagaagag gaagcagaac ggggaccggc aggggaagat
     3721 aaagacgtgg cgcgctgttt acggaatact tccgcccgct gtgcctgtgt ggtcagcacc
     3781 aggtgaaaac ggcggcccgc gccgtccgtc acccccgtca ccgcgcccgc cacatcaccc
     3841 tccgccgccc ggtgaaaggc cagcgtccgc ccgaagccgt ccaccacgcc cgtcagcacc
     3901 cggtatgcgg gcggctccgg cggcagcacc tcgtccgccc ccggcacccg ctccggccag
     3961 ctgagtatcc accacggccc ctgaaggctg tttgtcgcca ggtacacatg cggactcagg
     4021 cgaacatctt ccggcagcac ctgccagagc gcgcttagcg gctgcgaact gtgctgcgcc
     4081 gctactccgc cccgcgccag ccagagtgac tcgctgcggc tgtagcttat ctcgccggga
     4141 aacagcggct caaagtgaat gctcctgccg ccgctgtcgt tgagtatcag gccttcatcg
     4201 cgtatctgta agcggatatc gaagggcgct ttccagccgg gaccaaacac ccccaccggc
     4261 gcgggtgttc tggtccggta gctgctgtag gcgcgggaaa gaataaacgg cagcgggccg
     4321 ggcagcgcaa ggtccgtctc gcccggcagc accttcgcac ccagcagcgg gttcaccggg
     4381 ttagcatagg taatccctcc cggacatacc gaacaggcca ctcccgtcgg cgcacctatc
     4441 agcacccccg ctgaaccctg cacaatatcc agtcccttgc gggtcatatc accctgccgc
     4501 gccgccggtt ttccgcccat gaatcaattc cttctgcttt taatatggtt tatctggtca
     4561 ctgcaccagt tccgttttaa ttttttgcac ctgactcaaa attgccgcat atccggcatt
     4621 ccggggggta aatctttcgg cacaggcgca tccggtacag gttgtgtggc accacagttc
     4681 cagttaatca ctggcgcatc accgttcaca tccgactccc cttccagatg aatgtgcgta
     4741 ccattgagaa atatgcttcc gtccttcgtc agcaccagcc ttgccttacc acagcagagt
     4801 tcaagatgct caccggcaga ataaactgct gtttgtccgg tgttttcctt cattgtcttg
     4861 cccaccgaga aggtgacgtt attccccaca ttgacgctgt agcccattcc caccatcagt
     4921 gatttatgca gccctacctg tgaggactgc aacaacgcca ccgaggtatt cataatgccg
     4981 cctaccgtcg tctggtaagc cacacccacc gtcagcgcac gaatgatccc caccttttca
     5041 acctggtttg atcccacttt gataatctgg ttactgccca ccgtttccac ctggttgaca
     5101 ccaaccgtct gtatctgatt aacgccaatg ttctggatct ggttattggt aacggtgatc
     5161 gcctggttgt tacctatttt ttcggcatgg ttgtttatca catcagtggt gcggttattc
     5221 agcacctcgg tgttcatgtt cttctgcgcg tggatgtaga cctgttcttt ccctgtcgca
     5281 tcgtcaaact tcagttcatt aaacccgctg cccttatagg ttttcgagcg gatcgtcatc
     5341 tgcgtcttcg tccccggcag gctgccgggg gtgcggtttt cctggtggta ggtacgcccc
     5401 ataatgatcg gctggtccgg atcgccgttg aggaagtcca caatgacctc ctgacccaca
     5461 cgcggtatcg ccaggttacc aaagccggtg cctgcccacg cctgtgccac acggatccag
     5521 catgaacttt cctggtttga cgggttatag cggtcccagt taaatttcac ccgcacgcgg
     5581 ccatgttcat cacagaagat ttcctcgcct gccggtcccg tcacgacggc gctctgcggg
     5641 ccgtccacca gcggtttcag caacggctgt ggccgccatg tccggtctgc cgggattacc
     5701 gcaaagtggt tatccagcgt ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt
     5761 tcgccgtgaa gatcgctcgc caccacctgc cattcccggt tcaggttcgc ctgcggatgc
     5821 cccgtcagca caattcgccg tcccggccat atctccggcg aacggcttgt tccgcgcgcc
     5881 acttctgcgt tgttgcgcca gccatccatc tgccagcggg caaagttctg cccgtgggca
     5941 cccttgaaac gtccggggta gtcatacact tcatactgtg tgcgctggta atcctggtac
     6001 tggccttcct gatcaaaacg ccctgcccag ccgggtcgtt taaaggtgta gtctttggtc
     6061 accacggaag aagggcggat ttgtgcgctg tagcgaaact ggctgatgca gagggtgctc
     6121 acctcggtac gggtgttcgg attccagggg atctcaaagg actccggcag ataacgcacg
     6181 gtatcgcaca ggaccaggct ctggtcggta cttttttgcg cgtgctcctc ataaaagaag
     6241 atgccttcct ccgccgccat ccggcacagg aaatcgtaat cagtctcacc gtactggaca
     6301 caaaactcac gggaaggatg tggctcgctg aacagcgggc tccactcggt caccccgttt
     6361 tcctgcaata tcgtgccgag gatgctttcg atgtcctcat tctggaagat acggaagttc
     6421 tggcgcagcc ctgtgcgcca cagcggtggg cacaccttca tgctgtacag catctggttt
     6481 ttgtcgttct cccccagttc aaaccaggtc accatacctt tcacccggcg ctgtacgtca
     6541 tcgccctgcc atatcgtcag gtaggccatt ttgtcgagga tctgctggaa ttcaagggag
     6601 agaaactgct ggctgaccag agagagatcg agggaaaaaa gcgaagagag tgactggttc
     6661 agatgaaagg agaccaccgc aaaagcatcc ggtggcaggc cgtccacttc cagcgtgaaa
     6721 cgtaatccgg ttgacatagc aacctccggg gttaaggaaa aacagacagt tcgctgttaa
     6781 agcggctgtg acagtacagt gttacgcttg cgttcaggca cgctaccgcc gaagtttccg
     6841 gcagccgcca tctcccgtga acgtaacccc ttactgacaa cctttctttc tacggcgtaa
     6901 tacaaaaaca gcatccggcc tgatagccgg atgctgttgg cttaacttat gcttccagcg
     6961 gtgcgcgcca gtcgtcggca ccggaggtgc cagatttgac gtgttcccat tcaattttgc
     7021 ggtaggccag tgaaacttcg agcaactggg tgaactcgcg tttcgccggg tcctggcaat
     7081 gcggcatatg cagtttcata tcgacaatcg tcgaatcagt caggcgggta gtgaaataat
     7141 gctcctgttt gccttccaca gaggtacgcc accagtgaag ttcagtggtt ggcagcattt
     7201 cgccggaggc cagtgcgttg tacatcagcg gaaccgcttt gttcagcgcc acggtaaaaa
     7261 tgaaaggctt gtgagcacgc tgacccgcag gctgaccgga ctgaggatcg gtcgggacag
     7321 taacgttatg caggaattcc tgcaccagca tctcgtcttc gtgcccctgc acatagatgt
     7381 tgccgacaga atcagcggta aatgcacctg cagtaatatt tccctgggtc tgaccagtga
     7441 tggagatata acacggtgtt ggcatttgta aactccttgt taacgtggtt aattactact
     7501 tacaatgacc tgccgttaat cccagacagg cagatgcagc tcacttcacg gaaccgcatc
     7561 atttttcagt attttttaca tacaaaccga gctagaattt acggttgtcg taaaaactat
     7621 ttttaaaatc attaaatctc ttattcagca ataacttaat aatatata
//
LOCUS       DAPHJE010000169         7301 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPHJE010000169 DAPHJE010000000
VERSION     DAPHJE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.171"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(7..486)
                     /gene="folK"
                     /locus_tag="RH222_004070"
     CDS             complement(7..486)
                     /gene="folK"
                     /locus_tag="RH222_004070"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV3799005.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(483..1901)
                     /gene="pcnB"
                     /locus_tag="RH222_004071"
     CDS             complement(483..1901)
                     /gene="pcnB"
                     /locus_tag="RH222_004071"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV3799006.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1940..2866)
                     /gene="gluQRS"
                     /locus_tag="RH222_004072"
     CDS             complement(1940..2866)
                     /gene="gluQRS"
                     /locus_tag="RH222_004072"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV3799007.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2903..3358)
                     /gene="dksA"
                     /locus_tag="RH222_004073"
     CDS             complement(2903..3358)
                     /gene="dksA"
                     /locus_tag="RH222_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV3799008.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3536..4240)
                     /gene="sfsA"
                     /locus_tag="RH222_004074"
     CDS             complement(3536..4240)
                     /gene="sfsA"
                     /locus_tag="RH222_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV3799009.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4255..4785)
                     /gene="thpR"
                     /locus_tag="RH222_004075"
     CDS             complement(4255..4785)
                     /gene="thpR"
                     /locus_tag="RH222_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV3799010.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4859..7288
                     /gene="hrpB"
                     /locus_tag="RH222_004076"
     CDS             4859..7288
                     /gene="hrpB"
                     /locus_tag="RH222_004076"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV3799011.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1657 a         1904 c         2047 g         1693 t
ORIGIN      
        1 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
       61 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      121 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      181 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      241 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgagcgaagt
      301 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      361 gcggtaaaac gaagaaacgg caagaatgtg gctttcaggg atatcgccta atgctttcag
      421 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      481 tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acggcgacgc
      541 ggtgacggct cttcatccaa ctcgttaagc atcccttttt gatctggtgg cgcggaaacc
      601 tggaactcac cccaccattt caccagacgc tgcagttcag cgttacgttc aacttcagct
      661 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      721 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      781 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      841 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg caatcttctg tgccgtctcc
      901 agcagtgggt accagaacat ggcggcaaac aggaacgccg ggttcacgcg catatcgtta
      961 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1021 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1081 cacaacagct tataggtatc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1141 aacaggcgtg ccggtgggat atcgttcaac agggtagcga ggcgagggat cggttctgcg
     1201 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1261 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1321 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatagagg
     1381 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1441 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1501 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1561 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1621 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1681 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1741 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt
     1801 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1861 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     1921 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     1981 actcgggcac ggcggtaagc cgccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2041 tgaaatcctg ccagtgtgct tctgcctgct gtcccagaaa ttgaagtgcc gcgattagta
     2101 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2161 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2221 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2281 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2341 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgccgcgca
     2401 gctggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2461 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2521 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2581 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2641 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2701 cttctatgcg taccagccag cgaccttgcc gggcgcgagc ctgcaaatag ctgccgagcg
     2761 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2821 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     2881 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     2941 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3001 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3061 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3121 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3181 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3241 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3301 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3361 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3421 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3481 gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3541 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3601 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3661 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3721 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3781 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3841 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttctgcgc
     3901 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     3961 ggatagcctc tttcgtcaac ctgttagccc aaagcgtgtt gacgcaaata aatgcgccgc
     4021 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4081 aataccagac ggtatcgcca ggcgttgcac aaccggtcat tgcacccgta ttcgggcagt
     4141 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4201 gaattagcgt cgcgcgctgt agagggggag agaattccat tgcgacaatc cttgttattg
     4261 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4321 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cctggcggcg ggattgtcac
     4381 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4441 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4501 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4561 cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4621 ctcggcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4681 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcggatttc
     4741 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4801 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4861 gtcgtcgttg cccgttgctg ccgtcttatc tgaattactt accgctctcg attgtgcgcc
     4921 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     4981 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5041 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5101 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5161 aggcgtgctg acgcgcatga tccagcgtga cccggaactg agcggtgttg gactggtgat
     5221 ccttgatgaa tttcatgagc gcagcttgca ggcggatttg gcgttggcgc tgttactcga
     5281 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta ccctggacaa
     5341 cgaccgcttg cagcaaatgc tgccagaagc gcctgtcgtc atctcagaag ggcgctcgtt
     5401 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg atgccgttgc
     5461 ggtagccacc gctgaaatgc tgcgtcagga aagcggatca ttactgttat ttttacctgg
     5521 cgtcggagaa attcagcgtg tgcaggaaca actggcttcg cgcatcggca gtgatgtatt
     5581 gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga tcctcccggc
     5641 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5701 tgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg
     5761 cacggggctt acgcgactga ttactcaacg cgttagccag gcatccatga cgcagcgtgc
     5821 cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat ttaatcgcca aagaacaagc
     5881 agaacgcgcc gcggcgcaaa gtgaaccgga gatcttacaa agcgatcttt ccggtttgct
     5941 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6001 gccagtagtg aatctactgg ccgcgaaacg tctgttacaa atgctggggg cactggaggg
     6061 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6121 ggcaatgctg gttagcgcga agaacgacga cgaagctgct accgcggcaa aaattgccgc
     6181 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6241 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgtggcgg
     6301 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6361 tcgtcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6421 tgccaacgac gcgctaagcc gccacgaatg gttgatcgca ccgttattat tgcagggcag
     6481 cgcctcgccg gatgcgcgga ttttactggc gctgccggtc gatattgatg agttagtaca
     6541 acgctgcccg cagctggtac agcagtctga cactgtggag tgggatgacg cgcaaggtac
     6601 gctgaaagcc tggcgtcggc tgcaaatcgg tctgctgacg gtgaaagtgc agccgctggc
     6661 gaaaccctcg gaagacgagt tgcatcaggc gatgcttaat ggcatccgcg ataaagattt
     6721 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6781 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttat tggcgacgct
     6841 ggaaacgtgg ctgctgccac atatgactgg cgtacattca ctacgcggcc tgaaatcact
     6901 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     6961 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7021 agataacccg cccgcgctgg cggtgagaat gcaagagatg tttggcgagg ccaccaatcc
     7081 gacgatcgcc caggggcgcg tgccgctggt gctggagttg ctttcacctg cccaaaggcc
     7141 attacaaatc acacgagatt tgagcgcctt ctggaaagga gcgtaccgtg aggtgcaaaa
     7201 agagatgaaa gggcgttatc ccaaacatgt ctggccggac gacccggcaa atactgcacc
     7261 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct c
//
LOCUS       DAPHJE010000170         7264 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPHJE010000170 DAPHJE010000000
VERSION     DAPHJE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.172"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(319..1455)
                     /locus_tag="RH222_004077"
     CDS             complement(319..1455)
                     /locus_tag="RH222_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3799012.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1458..1820)
                     /locus_tag="RH222_004078"
     CDS             complement(1458..1820)
                     /locus_tag="RH222_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV3799013.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2357..4270
                     /gene="mtlA"
                     /locus_tag="RH222_004079"
     CDS             2357..4270
                     /gene="mtlA"
                     /locus_tag="RH222_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV3799014.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4500..5648
                     /gene="mtlD"
                     /locus_tag="RH222_004080"
     CDS             4500..5648
                     /gene="mtlD"
                     /locus_tag="RH222_004080"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV3799015.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5648..6235
                     /gene="mtlR"
                     /locus_tag="RH222_004081"
     CDS             5648..6235
                     /gene="mtlR"
                     /locus_tag="RH222_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV3799016.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6247..6456)
                     /locus_tag="RH222_004082"
     CDS             complement(6247..6456)
                     /locus_tag="RH222_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799017.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6741..7103
                     /locus_tag="RH222_004083"
     CDS             6741..7103
                     /locus_tag="RH222_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV3799018.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1845 a         1827 c         1844 g         1748 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
      181 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      241 catcgcagct aatgaagtaa tatgcaaaaa taattagtcc ttacaaggag caaagttcca
      301 taatctcccc cctccccctc aatgatccaa ataaagataa tgcatccagc tggtcattct
      361 tagcagcact ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ccgccacctg
      421 ggcgtagatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 cagcacaccg tccgtgccgg gcacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact ggtcagtttg ccgtggaaca cctgcccagg
      601 tagcgcgtta aacaccactt ctgcatcatc accaggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aaaaccatca ccggacgcaa
      721 cggcaaggca gctgcgtatg tgcctgggcg gatcagtacc tgagtgacgt agccattgct
      781 tggtgcgcga atgacagtct gctcaagatt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca gataacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 tgtgagctgc gcgcgcagag tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 cctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 ggggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc acccagcgcc gccgtcgcca gcgtccactg
     1381 attcaccgga atacgaaaga ttttaaagac cgcccacgcc agcgccacgt aagttaaaac
     1441 aatcaataga tccataatta gatctccggg aacgtggaat agtcagtctt tttctcggcg
     1501 gcgagttgat gctccagccg ggaaattcgg tcagaaagtg cggcgatttc cgggtcagtc
     1561 gctttctcct gcgacgcaac atgtgactgc ataccccagc cacgctccgg ttgatagagc
     1621 gtcgcccaga tccacagaaa cggccagata acatgcagag taaacaggct cacccagccc
     1681 gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt tggcaatcag ataagggatg
     1741 tcatgtatcg cgatgattcc ataaaaaatc accagaaaga cgaagatcag cactcccaac
     1801 gcgaaatagt ttagaaacat atctgcctcg gattcacgtt tatcagtgtt gtttttgggc
     1861 tggcagccag aagggagtca ggctgatatt ttgacaataa tccgggttcg cgattctcgc
     1921 cataacacca aagaataatt tttagaggtg atgagttgct tagttacata acgattgtat
     1981 gacgaaggca taacatgctg tagatcacat caggtgaacg ccgtaagaaa atatcttgtg
     2041 attcagatca caaagattca acaaaccatc aaaacaaaaa tgtgacacta ctcacattta
     2101 aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt tacacaagcg ttttgtgatg
     2161 aacgtcacgt caattacctc tctaccccct atatttatgt gattgatatc acacaaaagg
     2221 ccgtcgactg gacagttaac cgattcagtg ccagatttcg cagtatctac aaggtccggc
     2281 tacctctgcc gccacattaa caaaaaacct cgggcttcca gcctgcgcga cagcaaacat
     2341 aagaaggggt gtttttatgt catccgatat taagatcaaa gtgcaaagct ttggtcgttt
     2401 cctcagcaac atggtgatgc caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc
     2461 gttatttatt ccaacagggt ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat
     2521 gatcacttat ctcctgccgc tgctgatcgg ttataccggt ggtaagctgg taggcggcga
     2581 acgtggcggc gtagtcggtg ccatcaccac catgggcgtt atcgtcggcg cagacatgcc
     2641 gatgttcctc ggttctatga ttgcaggtcc gctgggcggc tggtgcatta agcacttcga
     2701 ccgctgggta gacggtaaga tcaaatccgg ttttgagatg ctggtgaata acttctccgc
     2761 aggcatcatc gggatgatcc tcgctattct ggcattcctc ggcattggcc cgattgttga
     2821 agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg gttgtccatg acatgctgcc
     2881 gctggcgtct atctttgttg aaccggcgaa aatcctgttc ctcaacaacg ccattaacca
     2941 cggtatcttc tcgccgctgg gtattcagca gtcccatgaa ctgggtaaat caatcttctt
     3001 cctgattgaa gctaacccag gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg
     3061 tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc atccacttcc tggggggtat
     3121 ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct
     3181 cggcggtatg actggcgtgt tcacgctgac tatcctgggc ggtggtctgg tttctccggc
     3241 atctccgggt tctatccttg ctgtactggc gatgacacca aaaggtgctt atttcgctaa
     3301 catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa
     3361 aaccagcaaa gtgaaagaag aagatgatat tgaagcagca actcgtcgta tgcaggacat
     3421 gaaagctgag tctaaaggca catctccgct gtctgcgggc gatgtgacta acgacctgag
     3481 ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc
     3541 aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca cagatttcgg tcactaacag
     3601 cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc actcaccgtg acctgaccga
     3661 acgcgctatg cgccaggttc cgcaggcgca gcatatttcg ctgaccaact tcctcgacag
     3721 cggcctgtac accagcctaa ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga
     3781 agtgaaagtg aaagacagtc tgaaagacag ctttgacgat tccagtgcta acctgttcaa
     3841 actgggcgcg gagaacatct tcctcggtcg caaagcagca accaaagaag aagcgattcg
     3901 ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat
     3961 gctggatcgt gaaaaactga ccccgactta tctgggtgag tctatcgcgg tgccacacgg
     4021 tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga
     4081 aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt ctggtgattg gtattgcagc
     4141 ccgtaacaac gagcacattc aggttatcac cagcctgacc aatgcactgg atgatgagtc
     4201 cgtcatcgag cgtctggcac acaccaccag cgtggatgaa gtgctggaac tgctggcagg
     4261 tcgtaagtaa tccaatccca ccctctccac atggagaagg tggggttaat tgcctgatgc
     4321 gctacgctta tcaggcctac aggatgcatc acaatttgtt gaatttgcac gttcttgtag
     4381 gccggataag gcgcttacgc cgcatccggc gcggcccctc tcctcacgga gagggtttgg
     4441 gtgagggaaa agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta
     4501 tgaaagcatt acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg
     4561 cagacgcggg tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga
     4621 atgcccgtca tagctatcag gttcatgtgg ttggtgaaac cgagcaggta gataccgttt
     4681 ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggtcg
     4741 atttagtcac taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca
     4801 aagggctggt gaaacgcaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg
     4861 aaaacatggt acgcggtacc acgcagctga aaggccatgt gatgaacgcc ctgccggaag
     4921 acgccaaagc gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg
     4981 taccgccttc ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg
     5041 aatggattgt cgataaaacg cagttcaaag gcacactgcc gaacatccca ggcatggagt
     5101 taaccgacaa cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg
     5161 ctataaccgc gtacctcgga aaactggctg gtcatcagac cattcgtgac gctattctcg
     5221 acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca
     5281 agcgctacgg ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt
     5341 ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac
     5401 tgagtgctgg cgaccgtctg atcaagccac tgctcggtac gctggaatac agccttccgc
     5461 acaagaatct gattcagggg attgctggtg caatgcactt ccgcagtgaa gatgatccgc
     5521 aggctcagga actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga
     5581 tttccggtct tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa
     5641 tgcaataatg gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat
     5701 gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc
     5761 tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa
     5821 tcttctggtg cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc
     5881 gttactcgac ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg
     5941 gttgggcgtc attaaccgcc aggaatacga agatgcggag ctgctgatgg cattgcgtga
     6001 agaactaaat cacgacggca acgagtacgc ctttaccgac gacgagatcc ttggaccctt
     6061 tggtgaactg cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc
     6121 cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct
     6181 ttcactgact gagctgattt ccagaatcag cttaaaaaaa gcctttcaaa agtaagcaac
     6241 gtctgcttac tgcccctcta cctgcttcgg ccgataaagc cgacgataat actctagacg
     6301 ttgtagatat aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg
     6361 aagttttttc agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc
     6421 gataagaagc ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc
     6481 gccgggaatg cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa
     6541 aacgtgctga atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc
     6601 ttaattaatc aacgaattgc gtagtttttg agcatgttaa tgattacgtt gcagaactat
     6661 tacaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta
     6721 tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg
     6781 atcgcctgga cgccatccgc caccagcagg ccgatctatc gctggttgaa gccgcagaca
     6841 aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg
     6901 aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc
     6961 gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac
     7021 tggtggttgt gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg
     7081 gattctctaa gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca
     7141 tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa
     7201 caattcactc cctttacgtc gatttaccgc tggttgatat tctgaaaatg catttttacc
     7261 gaaa
//
LOCUS       DAPHJE010000171         7251 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPHJE010000171 DAPHJE010000000
VERSION     DAPHJE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.173"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..672
                     /locus_tag="RH222_004084"
     CDS             <1..672
                     /locus_tag="RH222_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HDV3799019.1"
                     /translation="TTKAIKQKITPSDIYRHALSGNILLSVYFQSPVILKKIQTFNGK
                     IKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCTEARYICPVQQIIDTPLLRKLNQFRTF
                     VRNVRPGDELDVPAQVSEKNLTPPPGNSSGNLEQQIASTSQLIGSLLAEDMNSEQAAN
                     IARGWASSQASGVMTDWLSRFGTARITLGVDEDFSLKNSRDGNPRKWRFDATNSELCA
                     ERHEC"
     gene            599..2720
                     /locus_tag="RH222_004085"
                     /pseudo
     CDS             599..2720
                     /locus_tag="RH222_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(2890..3942)
                     /locus_tag="RH222_004086"
     CDS             complement(2890..3942)
                     /locus_tag="RH222_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDV3799020.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKYLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERIFSFSIAENIIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKHS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVLESVIVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            4257..5573
                     /gene="shiA"
                     /locus_tag="RH222_004087"
     CDS             4257..5573
                     /gene="shiA"
                     /locus_tag="RH222_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV3799021.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            5675..7129
                     /gene="amn"
                     /locus_tag="RH222_004088"
     CDS             5675..7129
                     /gene="amn"
                     /locus_tag="RH222_004088"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV3799022.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAIAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         1924 a         1706 c         1684 g         1937 t
ORIGIN      
        1 actaccaaag ccataaaaca aaaaattact cctagcgata tttatcgtca tgccttgagt
       61 ggcaacatcc tactatcggt ctatttccag tctcctgtga tacttaagaa gatacaaact
      121 tttaatggaa aaataaaatt caggcagttt gtgggagacc tacttgataa actatgcatg
      181 cttgacaggg acggttttat ttatggtcaa aatttaagac tctgtacaga agctagatat
      241 atttgtcctg tgcaacaaat tatagatacc cccctgttac gcaaactcaa ccagtttcgt
      301 acgtttgttc gtaatgtccg cccgggtgat gaactggatg tcccggcaca agttagtgaa
      361 aaaaatttaa ccccgccgcc gggtaacagc agtggcaacc ttgagcaaca gatagccagt
      421 acttcacagc taattggatc tctgctcgcc gaagatatga acagcgagca agcggcaaac
      481 atagcacgtg gatgggcctc ttctcaggct tcaggggtaa tgacagactg gttaagccgc
      541 ttcggtaccg caagaatcac gctgggcgtg gatgaagatt tcagcctgaa gaactcccgt
      601 gacggcaacc ctcgaaaatg gcgattcgat gcaacaaaca gtgaactatg tgccgaacgt
      661 cacgaatgct gaaatcacgc tggcagcctc gaaggacccg gtgattgccg acaataacga
      721 tctcacgaca ctaacagcac cgtcgctgat acagagggca atgcgatagc caacactgag
      781 gtaacattta ctctaccgga agatgtgaag gcgaacttca cgctgagcga tggcgacaaa
      841 gcggttactg atgccgatgg caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct
      901 cataccgtga cagcatcgat ggttggcggt aagagtgagc agttggtggt gaactttact
      961 gcggatacgc tcactgcgca ggttaatctt aacgttaccg aggacaattt catcgccaat
     1021 aacattggaa tgaccaggct acaggcaaca gtgactgatg gaaacggcaa cccagtagaa
     1081 ggtataaaag ttaatttccg tggaacctcc gtcacgctaa gcagcaccag cgttgaaacg
     1141 gatgatcagg ttttcgctga aattcttgtg acaagcaccg aggtcggact aaaaacagtt
     1201 tcagcctctc tggcagataa acctactgaa gtcatctcgc gattactgaa tgccaaggta
     1261 gatgttaatt ctgcgacgat taccagtcag gagatacctg aaggtcaggt catggtcgca
     1321 caagacatag cagttaaagc tcacgttaac gaccagtttg gcaacccggt gacgcatcaa
     1381 cccgcgacat tcagtgcagc gccatcctcg caaatgatca tcagccagaa tacggtctct
     1441 actaatacgc agggagtagc cgaggtcacc atgacgcctg aaagaaacgg ctcgtatact
     1501 gtaaaagcat ccctagcgaa tggagcttca cttgagaaac aactggaggc tattgatgaa
     1561 aaactgacac tcacgtccag tccgcttatc ggtgtcaatg ctcctaaagg cgctactctg
     1621 acggcaatgc taacctctgc aaatggcact ccagtggagg gtcaggtcat caactttagc
     1681 gtaacgctag aaggtgcgac gttaagtggc ggaaaagtga gaaccaactc ttcaggtcag
     1741 gctccagtcg ttctgaccag caataaagtc ggtacatata cggtgactgc atcgttccat
     1801 aacggcgtca caatacagac acagacaacc gtgaaggtta ctggcaaccc aagcaccgca
     1861 catgttgcta gctttatcgc tgaaccgtcg actatcgccg ccaccaacag tgatttaagt
     1921 accttaaagg caacggttga ggatggcagt ggtaacctga tcgaaggtct cactgtgtac
     1981 ttcgccttaa aaaacggctc caccacatta acgtcattaa cagcggtgac cgatcaaaac
     2041 ggaatcgcga caacaagcgt gaaaggagcg ataacgggga gcgtcactgt aagcacagtc
     2101 acaagcgcag gtggaatgca aacagtagat atatcgctag tggcaggccc agcagacgcc
     2161 tcgcagtcca tccttaagaa caaccagtca tcactgaaag gggactttac cgatagtgct
     2221 gagctacatc ttgttctgca cgatatatca ggcaatccga tcaaagtttc tgaagggatg
     2281 gaatttgtgc aatcaggtac aaacgtgccc tatatgaaaa ttagcgcaat tgattacact
     2341 caaaatatca acggtgatta caaagctact gttacaggag gcggagaggg tatcgcaacg
     2401 ctgatccccg tattgaatgg tgttcatcag gcgggcctga gtaccacgat agaattcatt
     2461 agcgcggaaa ccagaccaat gaccggtaca gtgtcagtga atggtgcaaa cctaccgaca
     2521 gcttcattcc cttcgcaggg atttaccggg gcgtattatc agttgaataa tgacaacttt
     2581 gcaccaggaa aaacggcggc tgattattcg tttcaaactc ggcctcttgg gtaggcgttg
     2641 atgctactgg taaagtgaca ttcaaaaatg acggtgatag caatactgta ataatcacag
     2701 cgcccccaag atccggtggt gctatttacc aaacagttcc gcatgaatcg cgttcggtgt
     2761 gactatgatc attttcggtg agtattgctt tgatccaaag gctggttttc tgtgatgatt
     2821 gaccagcctc agagtattga tgacaccacc gttcataaca gatcggcatt actatgcata
     2881 agtactgcct caaattctcg ccaaaacact ttttaatgcc tttgcgaatt catccagact
     2941 tatattgtca attttttgcc caacgatgac tgactccaaa acatcggcca ttgcgggcca
     3001 tcgcgttttg taatcagcat aaattgcaac aacaggggta ttataggcac ttgccatatg
     3061 taatattgag gtgtcaggag ttattgcaat ataagcatct tttatgattg ctgctgaatg
     3121 tttaatagag ggtgataagg ataaacggta gacattatta taacaatcca ctaatgccct
     3181 ggctttatct tcccctttgg gtccataaac gatcactatt ggaatatctg tttcactttg
     3241 aatttttgct attatatttt ctgcaatcga aaatgaaaat atacgttcct gcgagctacc
     3301 ttctaaattg agcgcaatgt aagatcctaa cgatcgcatt tcctcgcgta cctcatcgag
     3361 tacatcatcg cttaatggca attcatatat tgacctgact gccggaaaac ccgcctcacg
     3421 catcataaat gcccatgtca tgggtacagg agcccggaga ttctgatcca tccgggacgc
     3481 gttcttacac aaaggggaat agcacttcat ggttaaaccg acgacctgga aattcgtttt
     3541 tgctttcaac tgactgataa atatcatcgt tttaaggttc tttttacgca tcgcctctat
     3601 acaaagatcc ggcgtaccgt attgcagtgt tatgttcttc gctaaatatt ttatttcttt
     3661 taatgttgcg tgatcctgca tagtcattgt gactaatgtt aatttagtct gttcaagttt
     3721 gagcgcatta aagacttcta aattaattgt cgacgtaaca attaatagat gcttaatttt
     3781 atgcaattca agcgcccgaa taacaggaaa gatggccata gcatcgccaa tctgatcggg
     3841 aatatgtatg acaacaaagt ctgttttttc aatattaaaa ttataagctt tataatcgta
     3901 gtaactaaat gcaatacgtc tcaacaatga tactaaaaac ataactaacc tcgcctccct
     3961 actggttata atgcaatgca gtctatcaga atcatcaggg tacaattttg tgcatatgcg
     4021 gacttttatg tttcatatct cgaacctgtg ggccctctgc ttaatcctta aacaacacca
     4081 gcaactcctg cgctttcatc ttccatcgaa tttttcatgt tgccgctaat cagccataaa
     4141 atcatttgca gatgcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc
     4201 aatgtgagtt accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg
     4261 actccacgct catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac
     4321 gagctgcgtt aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg
     4381 gcattaccgc cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg
     4441 gaacgctcgc cgcatttgcc acctttggtg tcggcttcct tttccgaccg ctcggcggtg
     4501 tcattttcgg tcactttggt gaccggctgg gacgtaagcg catgttaatg ctgaccgtct
     4561 ggatgatggg catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt
     4621 ggtgggcacc tattttgctg gtgacactgc gtgccattca gggatttgcc gtcggcggcg
     4681 aatggggagg cgcagcgttg ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt
     4741 acagtagcgg tgtacaagtt ggctacggag taggtttact gctttcaacc ggactggttt
     4801 cattgatcag tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt
     4861 tcctgtttag catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt
     4921 ccgcggaatt tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta
     4981 tcgaagctct gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg
     5041 agttgctgac gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg
     5101 ggttaccgcg cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga
     5161 caattccctg ttttgcctgg cttgccgatc gttttggtcg gcgcagggtt tatatcacag
     5221 gcgcgttaat cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcca
     5281 ttttctggat agttttcttc tccatcatgc tggcaaacat tgcgcatgac atggtggtgt
     5341 gtgtgcaaca accgatgttt accgaaatgt ttggtgccag ttatcgctac agtggtgctg
     5401 gagtcggtta tcaggttgcc agtgtggttg gcggtggatt tacacctttt attgccgctg
     5461 cactcatcac ttactttgcc gggaactggc atagcgttgc catttatttg ctggctggat
     5521 gcctgatttc tgcaatgacc gctttattga tgaaagacaa tcaacgctcg tgatagcctg
     5581 gcgaagatca tccgatcttc gccttatact tttgtttcac attcctgtga catactatcg
     5641 gatgtgcggt aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac
     5701 cccagctcag gcactggata aactcgacgc gctgtatgag caatctgtag ttgctttacg
     5761 caacgccatt ggcaaatata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca
     5821 aggtcttttt gtctatccat cactgaccgt aacctgggac ggtagcacaa ctaatccccc
     5881 caaaacgcgc gcatttggtc gttttactca cgcaggcagc tacaccacca cgattactcg
     5941 ccctactctc tttcgttcgt atctcaatga acaacttacg ttgctgtatc aggattatgg
     6001 tgcgcatatc tctgtgcaac cctcgcagca tgaaatccct tatccttatg tcatcgatgg
     6061 ctctgaattg acacttgatc gctcaatgag cgctggttta actcgctact tcccgacaac
     6121 agaactggcg caaatcggcg atgaaactgc agacggcatt tatcatccaa cggaattctc
     6181 cccgctatcg cattttgatg cgcgccgcgt cgatttttcc ctcgcgcggt tgcgccatta
     6241 taccggtacg ccagttgaac attttcagcc gttcgtcttg tttaccaact acacacgtta
     6301 tgtggatgag ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat
     6361 tgccctttct tgtgctggcg ggaactggat cactgccgaa accgaagcgc cagaagaagc
     6421 catttccgac cttgcatgga aaaaacacca gatgccagca tggcatttaa ttaccgctga
     6481 tggtcagggt attactctgg tgaatattgg cgtgggaccg tccaatgcta aaaccatctg
     6541 cgatcatctg gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt
     6601 acgtgaaagc caggccattg gagattatgt ccttgcacac gcttatttac gcgatgacca
     6661 cgttcttgat gcggttctgc cgcccgatat tcctattccg agcattgctg aagtgcaacg
     6721 tgcgctttat gacgctacca agttagtcag cggcaggcct ggtgaagaag tcaaacagcg
     6781 gttacgtact ggcacggtgg taaccacaga tgacaggaac tgggagttac gttactcagc
     6841 ttctgcactt cgttttaact taagccgggc catagcaatt gatatggaaa gtgcaaccat
     6901 tgccgcgcaa ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa
     6961 accgttacat ggtgagatta agcttcccgg ccaggctaac cgtttttatg aaggcgctat
     7021 ttccgaacac ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg
     7081 actgcattca cgtaaattac gaacctttaa tgagccgccg ttccgataat aagttatgaa
     7141 ccttcttatc agaaatagat gtgaaggatt aagtaagaca tctggcaaat agcctgcaat
     7201 aaacgcgggc tgtttgcttc cttgggcaga tgcgagtttt attatcgtct t
//
LOCUS       DAPHJE010000172         7241 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPHJE010000172 DAPHJE010000000
VERSION     DAPHJE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.174"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            191..1357
                     /gene="ugd"
                     /locus_tag="RH222_004089"
     CDS             191..1357
                     /gene="ugd"
                     /locus_tag="RH222_004089"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV3799023.1"
                     /translation="MKITISGTGYVGLSNGVLIAQNHEVVALDIVQAKVDMLNQKISP
                     IVDKEIQEYLAEKPLNFRATTDKHDAYRNADYVIIATPTDYDPKTNYFNTSTVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTRDIKERLGIDNVIFSPEFLREGRALYDNLHPSRIVI
                     GERSARAERFADLLKEGAIKQDIPTLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESQGLNSKQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYESVPNNIIAAI
                     VDANRTRKDFISDSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGIPVII
                     YEPVMQEDEFFNSRVVRDLDAFKQEADVIISNRMAEELADVVDKVYTRDLFGND"
     gene            1550..2557
                     /locus_tag="RH222_004090"
     CDS             1550..2557
                     /locus_tag="RH222_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004144152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HDV3799024.1"
                     /translation="MKFLVTGAAGFIGFHVSKRLLNDGHQVVGIDNINDYYDVKLKES
                     RLEKLESLSFTFYKLDLADRDGMAKLFETEQFERVIHLAAQAGVRYSLENPYAYADSN
                     LTGYLNILEGCRHNKVQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYSIPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFT
                     YIDDIVEAIVRIQDVIPQPDPEWTVEEGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     QALGLEAKKNMMPIQPGDVLNTSAETQALYKTIGFKPETPVQQGVKNFVDWYKEYYQY
                     N"
     gene            complement(2907..4379)
                     /locus_tag="RH222_004091"
                     /pseudo
     CDS             complement(2907..4379)
                     /locus_tag="RH222_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            4415..4662
                     /locus_tag="RH222_004092"
                     /pseudo
     CDS             4415..4662
                     /locus_tag="RH222_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4765..5937
                     /gene="istA"
                     /locus_tag="RH222_004093"
     CDS             4765..5937
                     /gene="istA"
                     /locus_tag="RH222_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HDV3799025.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNSLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            5937..6734
                     /gene="istB"
                     /locus_tag="RH222_004094"
     CDS             5937..6734
                     /gene="istB"
                     /locus_tag="RH222_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HDV3799026.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQSGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVKSGQHQ"
     gene            complement(6864..>7241)
                     /locus_tag="RH222_004095"
     CDS             complement(6864..>7241)
                     /locus_tag="RH222_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDV3799027.1"
                     /translation="IILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHL
                     EFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNN
                     RPRKTLKFKTPKEIIERGVALTD"
BASE COUNT         1842 a         1752 c         1828 g         1819 t
ORIGIN      
        1 gaagagattt tagctcttct caagtaatgc attatccata atggaagcca taggatcaga
       61 ttttaatcgt attattgacc ttatggctta ttcttgacat cccactggtc aaagcgtaaa
      121 accgcccaca gtattattta aattcgtggc tatccgccat tacccagtta actgattaaa
      181 gtgtaatctc atgaaaatta ctatttccgg tacaggttat gttggtttat cgaacggtgt
      241 cctgattgcg caaaaccacg aagtggttgc cctggatatc gtacaggcca aagtggatat
      301 gcttaaccag aagatctctc cgattgtgga taaagagatt caggaatatc tggcagaaaa
      361 accgttaaat ttccgtgcca ccacggacaa gcacgacgcc tatcgtaatg ccgactacgt
      421 gattattgcg acgccgaccg attatgatcc caaaaccaac tacttcaaca cctctacagt
      481 ggaagcggtt attcgcgatg tcacagagat caacccgaac gcggtgatga tcattaaatc
      541 gaccatccct gtggggttta cccgcgacat caaagaacgt ttagggatag ataatgttat
      601 tttctctcct gagtttctgc gtgaaggccg tgcgctgtac gacaacctgc acccgtcgcg
      661 cattgttatt ggtgagcgct ctgcgcgtgc tgaacgtttc gcagacctgt tgaaagaagg
      721 cgcgattaag caggatatcc cgaccctgtt taccgactcc actgaagcgg aagcgatcaa
      781 actgttcgcg aacacctatc tggcgctgcg cgttgcctat ttcaacgagc tcgacagcta
      841 tgctgaaagc caggggctga acagcaagca gattatcgaa ggggtatgcc tggatccgcg
      901 tatcggcaac cactataaca acccgtcctt tggctatggc ggctactgcc tgccgaaaga
      961 taccaagcag ctactggcga actacgaatc ggtcccgaat aatatcattg ccgctattgt
     1021 ggacgccaac cgtacccgta aggactttat ttctgattcc atcctcgctc gtaagccgaa
     1081 agtggtgggt gtatatcgcc tgatcatgaa gagtggttcg gacaatttcc gcgcctcttc
     1141 tatccagggc atcatgaagc gcatcaaggc gaaaggtatt ccggtcatta tctatgaacc
     1201 ggtgatgcag gaagatgagt tcttcaactc acgcgtcgtg cgcgacctgg atgccttcaa
     1261 acaagaggcg gatgtgatca tctctaaccg tatggcggaa gagttggcgg atgtggtgga
     1321 caaggtttac acccgcgatc tgtttggtaa tgattaggat ttataacctt tggctggatg
     1381 agaatatctc cagccaaatt ttatatgaaa ttggatttca ttgatgttaa ttaacttgtg
     1441 gaaatggtta tccagttagt tatttacggt tagtgagttt ttctggtgat ataaacaaag
     1501 tttgtaaaac atcaatgctg ccaatcgagt atttctcagg agtttaaata tgaaattttt
     1561 agtcactggt gcggctggtt ttattgggtt tcatgtcagc aaacgccttt tgaatgatgg
     1621 gcatcaagtt gttgggattg ataacattaa tgattattat gatgtaaagt taaaagaatc
     1681 tcgtcttgaa aaattagaat ctctctcttt tactttttac aagttagatc tcgctgaccg
     1741 tgatggtatg gctaaacttt tcgaaacgga gcagtttgaa cgtgttatcc atcttgcggc
     1801 tcaagcgggt gtacgttatt ctctcgaaaa tccatatgct tacgcagatt ccaatttaac
     1861 gggctacctg aatattttgg aaggttgccg tcataataaa gtgcaacatc tattatatgc
     1921 ttcatccagt tctgtctacg gtctaaaccg taaaatgcca ttctcaacag atgattcagt
     1981 cgaccacccc gtttcgttgt atgctgccac aaaaaaagct aatgagctga tggcacatac
     2041 ttattcccat ctgtacagca tcccgacgac aggactgcgc ttctttacgg tctatggtcc
     2101 ctggggccgt ccggacatgg cgttattcaa attcacgaaa gcgatgctgg aagggaaaag
     2161 cattgatgtt tataactatg gtaaaatgaa gcgtgatttt acctatatag atgatattgt
     2221 cgaagctatt gttcgtatcc aggatgtaat ccctcagcct gatcctgaat ggacagtcga
     2281 ggaaggctct ccggctacca gctccgcgcc atatcgggtc tacaacattg gtaacagctc
     2341 accggtggaa ctgatggatt atatcaatgc attggagcag gcattgggat tggaagcgaa
     2401 gaagaatatg atgcctatcc agccgggtga tgtgttgaat accagtgccg agacacaagc
     2461 tctctataaa actattggtt ttaaaccgga aacgccagta cagcaaggtg ttaaaaactt
     2521 tgtagattgg tataaagagt actatcaata taactagttt attggaaggg ggggatagaa
     2581 acgttttcgc gcctccctca taccggcagc aagtagcgga tgtatcatta ccgtatggcg
     2641 gaaaagcggg tggatgttgc ggacaaggta tacacccgtg atctgtttgg taacgattag
     2701 ttgtgcctgt ctgacccagg cggttccctg ccgccagtgt tcttctttct ctcccccgct
     2761 atatccctca ctacgcgtat ttctcgcaca ttcagattaa cctcaataaa ttgtgatgga
     2821 cattacgatc ctgttcaaaa gccgtgcggg ttgaaaacag gttcgataag ttaaacgtaa
     2881 gcggctcgcc agaaccgtat tgatatttac tgagaggtca gatcaacttt ccagggcaac
     2941 agatcgcgta cccggtttgc cggccagtct tggatatgct caataacgta acgcagccat
     3001 ttttctggct ctacattgtt cagacggcat gtgccgatca gcgagtacaa caccgccgca
     3061 tgttcgccac cgctgtcgga acccgcgaag agccagtttt tccggcctac ggtcactccc
     3121 cgtaaggcgt tctctgcaat gttgttgtcg atttccaccc agccattact gcagtacacg
     3181 ttcagggcct cccactgttt cagcaggtat gcgaacgctt ttgccgtatc cgagtgacgc
     3241 gacagtgttt tcatctgagt ctgtatccag tcatacagtg actgcatcag tggcgcagct
     3301 ctggcttttc ttgtcgccag acgctgttct gctaaacatc cccggacttc tgcctcgatg
     3361 acatacagtt caccgatacg ctgcagggct tccgtggtga tgtcggttgg cactcttgca
     3421 tgcacatcgt gggtttttct ccgggcatga gccatacacg cggcttccgt tattctgccg
     3481 gattcgtata acacccggta accaccgtaa gcatcgacct gaaacacacc gctgtaaccg
     3541 gccagatgat tttgtggatg gatacctttc cggtccggtg agtacgcgaa ccagaccgcc
     3601 gggggcatct gtgaaccggc attacggtca tcacggacgt aggcccacag ccgggcagtc
     3661 cttgttttac cactgcccgg ctcctggacc ggaacgggga tatcatcggc atggacttta
     3721 ccgggcatca gcacatactg gcgcaggacg tcatacaacg gctccagcag ttcagcaaca
     3781 gcacctgtcc agcgccccag tgtggcacgg ctcagctcca ctccctgacg acgatatatt
     3841 tctgactggc ggtataacgg cagatggtct gcatattttc cagcgacaac atggaccaga
     3901 agccccgctc cggcataact gcgtgcaatg ggttttgaag gcaccggagc ctgcacgata
     3961 tggtcgcacc ggcaacaggc cagtttcgga cgttgcgttt cgataacctt aaaggcgctg
     4021 ctgataagct ccagttgctc tgacacatca catcccagag aactgagttc gccaccacag
     4081 gcaggacagc attcctcttc cggccggata acccgggttt cacggggaag tgaggccggt
     4141 aacggtttac gggctgaaga ctggcgcagg gcggatggca gtaccggatc atactgctca
     4201 cccagcgttt ccgccatttc ttcctgaagt gcactgattc gctcctgtgc ttcctgtatc
     4261 tgtcgttcgg tttttgcgcg aagtttttct gagcttttac cgaactgcat gcgctgcagt
     4321 tttgcaacca gcgccttcag ccggttgatt tcagaagcat aagccgccac ccgctgtgtg
     4381 tagtggcaca gtaagtttgg ccacctgatt aaaggtgata ttctcacctc aacacaaaac
     4441 aggtgactta atgaacaaga aaaccaaacg tactttcacc cccgagttca gactggaatg
     4501 tgcgcaactg attgttgata agggctactc atatcgacaa gccagtgaat cgatgaatgt
     4561 cggctctacc actcttgaga gctgggtacg tcagctcaga cgagagcgcc aggggattgc
     4621 ccctctgcca caccgattac tccagaccag caacgtatcc gcgtgtcagc gccagtgata
     4681 taagacggta attcaccatt tggattgtcc gctccaccca acatgttgtt tccttaaggt
     4741 tctcacacca gaaaggacat caacatgctg agcagagagg acttttacat gataaagcaa
     4801 atgcgccagc agggcgcgta cattgtcgat attgcgactc agattggttg ctctgaacgg
     4861 acggtcagac gctacctcaa ataccctgaa ccgccagcca gaaagacccg ccacaaaatg
     4921 gttaagctga aaccgtttat ggattacatc gacatgcgcc tggcagagaa tgtctggaat
     4981 agtgaggtta tcttcgcgga gattaaggca atgggttata cgggcggacg ttccatgctg
     5041 cgttactaca tccagcccaa acgtaaaatg cgtccgtcaa aaagaacagt tcgcttcgaa
     5101 actcagcctg gataccagct ccagcacgac tggggcgaag ttgaggtgga ggttgccggg
     5161 caacggtgca aagttaactt tgcggttaat tcgctggggt tctcccgccg cttccatgtc
     5221 ttcgcagcac caaaacagga tgctgagcat acctacgaat cactggttcg cgccttccgc
     5281 tacttcggtg gttgtgtgaa aacggtgctg gttgataacc agaaggctgc ggtgctgaag
     5341 aataacaacg ggaaagtcgt gttcaactcc ggattcctgt tgctggccga ccactataac
     5401 ttcctgccac gggcatgccg tccacgcagg gccagaacaa aaggtaaggt tgagcgaatg
     5461 gtgaaatacc tcaaggagaa cttcttcgtc cggtaccgca ggttcgacag cttcactcat
     5521 gttaatcaac aactggagca atggatagcc gatgtggctg acaaacggga acttcgccag
     5581 ttcaaagaaa cgccggaaca gcgcttcgcg ctggagcagg aacatctgca gccgttaccg
     5641 gatacggact tcgataccag ttacttcgat atccgccatg tgtcctggga cagctatatc
     5701 gaggttggtg gtaatcgtta cagcgttccc gaagcgctgt gtggtcagcc ggtatcgata
     5761 cgaatatcgc tggatgacga gttgcggatc tacagtaatg agaaactggt ggcctcacat
     5821 cgcctctgtt cggcatcgtc tggctggcag acagtgccgg agcatcacgc cccgctctgg
     5881 cagcaggtca gtcaggtgga acatcgacca ctgagtgcct atgaggagct gttgtgatgc
     5941 atgagctgga agtcctgctg agtcgcctga aaatggagca tctgagttat cacgttgaaa
     6001 gcctgctgga acaggcagct aaaaaagagc tgaactaccg ggagttcctg tgcatggcgc
     6061 tacagcagga atggaacggc aggcatcaga gcggtatgga gtccaggctg aagcaggctc
     6121 gtctgccgtg ggtcaaaacg ctggagcagt tcgactttac cttccagccg ggcatcgacc
     6181 gtaaggttgt ccgggaactg gctggtctgg cgttcgtgga gcgcagcgaa aacgtgatcc
     6241 tgctgggacc tcctggtgtc ggaaaaactc atctggccat agctcttggc gtgaaagcgg
     6301 tggatgcggg acatcgggta ctgtttatgc cactggacag actgatcgcg acactgatga
     6361 aagcgaaaca ggaaaaccgg ctggagcgtc agctgcagca actgagttat gcccgggtgt
     6421 tgatcctgga tgaaataggc tatctgccga tgaacagaga ggaagccagc ctgttcttcc
     6481 ggctactgaa ccgtcgatat gaaaaagcga gcatcatact gacgtcaaac aaagggttcg
     6541 cagactgggg agaaatgttc ggagatcacg tgctggcaac agcgatactg gatcggttgc
     6601 tacatcactc aaccacgctg aatatcaaag gagagagtta ccggttaaaa gagaaacgta
     6661 aagctggagt gctgaccaaa aacacaacgc caatcagtga tgatgaaatg gtgaaaagcg
     6721 gacagcatca gtaacgaaag tatcttagcg ggcatgaaaa tggcaaataa cggtcaaaca
     6781 tcgtggcgtt gacacgcgag ctggaaaagc aggttcgccg cctggaggaa caaaatacga
     6841 tattaaaaaa ggctactgta gattcaatct gtcaatgcaa cacccctttc aattatctct
     6901 ttcggtgttt tgaacttcag tgtctttctc ggtctgttgt ttagctgagc agcaaccaga
     6961 tctagttcat gttgagtata ttgggcaaga catgtctttt taggaaagta ctgccgaatt
     7021 agcccatttg tgttctcatt tgttccccgc tgccaaggac tctgaggatc gcagaagtaa
     7081 actttaacgc cggtgctgac agtaaattct agatgtctgg ccagttccat tcctctgtcc
     7141 catgtcagtg attttctgag ttctgacggt aaactcagga atttgtcggt aagagcctga
     7201 tttactgaga cagaatcttt gcccctgagt ctaaggatga t
//
LOCUS       DAPHJE010000173         7208 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPHJE010000173 DAPHJE010000000
VERSION     DAPHJE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.175"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..4574)
                     /locus_tag="RH222_004096"
                     /pseudo
     CDS             complement(1..4574)
                     /locus_tag="RH222_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(4594..5055)
                     /locus_tag="RH222_004097"
     CDS             complement(4594..5055)
                     /locus_tag="RH222_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HDV3799028.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5083..6984)
                     /locus_tag="RH222_004098"
     CDS             complement(5083..6984)
                     /locus_tag="RH222_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV3799029.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1336 a         2208 c         1957 g         1707 t
ORIGIN      
        1 tctgctttcc ttattacttt aattcctagc ttctccattt ctttagcaat attgtttatt
       61 tgactacgtg ataaacgaac cccatccagg aaacccgtat gcccactaac acgacgacca
      121 tcgccaatat agtccatcaa actgctgatt ttagaacttg cagccaatcc cagcggatca
      181 acccacccac acggatttgg cacataccgg taagtctgct caccccccag caaccccagc
      241 ggatcactca ccaggtacat cccggtttcc ggctcgtaat accggaaccg gttatagcac
      301 agcccggatt ccgcatcctg ccactgtcct gcatacagca ggccgggggc ggcttccggg
      361 tccagttccc cgcgcgggtc cgggtagccg gtgtctgcgg gcaggctgag tgccagcccc
      421 cacaggctgg tctgccccgg tcgccagacg gttttacctt cactgttaaa gagcatcagc
      481 ggacggcccg tcaggtcgct caccgcatga ttcgtccgcg tcacccactg cggggccacc
      541 gacggatgcg gctgccgtac ccggctgaac tgctggctta tcagctcaaa gccgttaaac
      601 accaggtgcc gtacgctgta cagtttatca tcgcggtatt cccggatttc cgcaatactg
      661 tcgccgtccc acagatacgt gaagcggatt ttcttccggt cgcagcgttt ctctgttcgt
      721 ctgccgctgg cgtcgtggcg gtattcccac agctcaccct gtgcgctgca atacccggtc
      781 agctggtcac ggctgtccca ccggaaccgc tctgtttccg gtcggtagcc gtcacggtgt
      841 ctggtgcggc tgaccatccg gcctgcggcg tcatagtcat actgcgtgtt tccggcctgt
      901 ttcagccggt ggcctccggc atactggtca gtctcttcac ttatccggtg cctgcccgca
      961 gactgcgcct tcaggtagcc gcagctgtca tagccatagg actcatcgcc ttcgccatag
     1021 cccagcccgg tgcctgaggc tgtcaccgcc atgacctggc cgttccggtc cagccggtac
     1081 tcccgccgtg tttcccgtcc gtaatcttcc cggctggtga cagccgacac attacccgcg
     1141 ctgtcgtaac ggtactcccg taccagtgag ggaaccagcc cgccccatgt ggcttcttcc
     1201 ggaaaaaatc cggcgcgctg gctgacaggc tgaccggctg cattccagcc ctgttcacag
     1261 gcaaaacccc ggttactgtg ccggagcact tcacgtcccg cctcatcacg gctgaacgtc
     1321 agcgtctcat cccccggcag gataacctct gccagttctc cggcggcgtt gtaacggtag
     1381 gtggttttca gcagggattc atcgggatgt ctgtcgcgtt catcttccgg tggcaggata
     1441 cagcggatga cggtgtgttc atcggggtag tggtattcaa tggcccgtcg gccctgagtg
     1501 gtgacattca cccgcccgcg ttcgtcatag ccggtgcgct gtgatatgcc gtccgggctg
     1561 aagatacccg tcagcctgcc ggatttgtca tacgcatact gcgttttgcc ttccggcgtg
     1621 atgatttctg ccaggcggcc tgcggcatcc cggctgtaga ggatggtttc ctccagaccg
     1681 ttaacctttg cggtatacag gccatcggcg tcatagcggt aacgccagac cacgccgccc
     1741 cagtcatgct cttccgtcac ccggccgcag ttatccagaa cataaagata tgactcaccc
     1801 tgcgggttga tgacttctgt cagttgcagc gtgtctttat cgtagcggta ctgccagcgg
     1861 tggccttcac cgtccgttcg cgccaccggc aaatcgaacg gaccgtattc catcgtccag
     1921 acaacgccgt taccgtccgt ccaggccagc aggttgccgt ggcggtcata gcggaatcct
     1981 tccgcgccgg ttccagcacg ttcaatgcca ctcagtttat ggtgctgctg ccactgtaac
     2041 cgcactgact gaccgctggg gccgcgcatc ttcaccggca ggtcgtgacc gctgtattcg
     2101 gtgtggtgtt cacggcccat cacgtcggtg agtgtttccg gccggttgtg gtggtcgtac
     2161 tctatgccca gacgccgttc atcctggcgg aatatccccg acagcagccc ctgcggcgtg
     2221 tacgccagcc gggtctcttc gccctgtggc gacaccagac ttaccggcag gccgcgttca
     2281 tcacactgct gcgtccacgt ctgtccggca gggtcggtga tgttgctgag caggccgcgc
     2341 tcatcatagc agtactgcca ctccgcgccg tctgccgccg tgtaacggct taactgaccg
     2401 tgtgcggcgt tgtattcaaa cttctccacc cgcccggcag gggagtgacg ggccagcagg
     2461 tgatggaatt catcccatta atacgtggtg gtgctgccgt ccggcgcttc ttcccgcaga
     2521 atattgccgt caggatcata gtaataacgg tgagtgccct tgccgtccgt cacggtggtg
     2581 cagccgtcac catagtccag cgtggcgttg taatagccct ccgccccggt gacggtcacg
     2641 caccggccct gttcatcgta ggtgaaacgg ctccacgtct ggtcgttatc ggaccagcgg
     2701 atgatccggt ccgcagcgtc gtactcataa aacaggtggt agtccagccg cgcatccgct
     2761 tctgtcagcc gcccccgcgc atcctgttcg taggtcgcca gcgtctgtat gccgttatcc
     2821 gtgcggtgaa tggctttcag gtagccgctg gcgtggtaca tcagtttaag ccggatcccg
     2881 tcggtgtgac tgacacccgt gatgcggtac tggctgtcgc gctcaaacgt gatgcggttg
     2941 ctggcggtgt cgctcatccc gcccagcata tacatccggt cgtcggggct gtgtgccagg
     3001 cggtcatcgc tggtatcgtc agtcaccggg tggcgcagac gcattcccgg aaaggcgtca
     3061 taaaagaagc ggctgctcag tgcatccctg tcgaacagct ccaggtaatc gtcgcggcgg
     3121 cgcagaatgt actgggcgtg atacgggtta accgccgtat cactgtcacc gtcgaaggcg
     3181 aagttcagcg tggctccctg gctgataata tctacccggt ttccctgttc acgcacccag
     3241 gcgtattcgc tccaggagtc gctccagccc acgccggtca ggccatgcag cggcagtaca
     3301 gaactgtgga aacgggtgaa ggagaggggg agtgtctggc ccagggtgaa gtcggtacgt
     3361 tcgtcgcagt acgcgccggt taccggatcg acaggatctg ttacccatct gatgacttta
     3421 ttggcaatac tttttaacga cttacctgtt ttccccagca acgcgccgag ctttgccccc
     3481 agcagcttcc cgcttttccc cagcaggttc cccagtttgc cgagtctcgc cagattcttc
     3541 agttttccca gcagtcccgc tggcggcaga agatagctgg ctatcgttat tccccccagg
     3601 atcattctct gccacggcgg gaactccggc tggatatcca gataggtcac ctgctcaccg
     3661 ccaataaaga cgtccggcga acctcccacg attgctgcgc tgcactccag tttgtcgccc
     3721 tggcgtgcgg caggctgatt gttgataaag acgttgctgc tgccctgggc tatcctcggt
     3781 ggtttgttct ccttgtcgca gagcgccgtg tcctcaccgg cttccgggaa atccacatct
     3841 tttttgattt cgccacgccc gcccaccatg gtggcaaact ccattaatac cggaccggcg
     3901 ggaaacagaa gcgatgcgcc ggcgctgacg cgatccataa aaccggcatt gctgtccgtc
     3961 agggtgataa cagcgttccc ccaggccttc gccagtggta ctgctttttc cgctgccgat
     4021 tcaagcaggt cagtggcaat atcaccccat gagggttccc cctgtgggac ttcaggtatt
     4081 gctccaggtg ggggaggagg gggaagggtg acggcggccc tggcagcggg ctttccgtta
     4141 atttttacgt tttcagaacc ggtattgatg gcgccgtccg tgtcacaaaa actgtttatc
     4201 catgactcaa gctggttctg tttctcctcc acataatcac tcgccttata gctggcatac
     4261 atcagcgcag gcaccagaac cagcgcataa gggccggtgg cacaggcggc cataatcagg
     4321 caaccttctg cgatggtcac tatcgccccc agcacggccc ctgccagcgc ccccaaaaaa
     4381 ctcttgtgct gaatttcatc gcccagcctg gcggcggggg ggccagactt ttttgatgcc
     4441 agcatctgtg caatggcttt gtcgttggct gcccgtttca tctggctggc gaccatcgcc
     4501 tgctgtgaca gcattcgcat tgccagcgta ccgcctgcgg tcgctccctg tggcccgcct
     4561 ggtccttcac tcatgtgtta tccctcgttt atgtcagcgc ggctggtcct tttggatgtc
     4621 attctccggt ggcaacgggg tgaagctctt tatcacctcc agcagcatcg tcttttgact
     4681 gttcttcagg gataaggagg tgctgaaagt gaaggtcagc agcgtatctc cacgccgctg
     4741 gaatacggct atctggctaa tccgcccctg attcccctca tcttccggac cgttccagga
     4801 ataatccagc agccaggccg gggcctctgc cagagtgatt tcccggcagg catgttcggt
     4861 gtacccatca agtttgccct ggtacaacgc cagcgtttcc tggtacagct ctggtgtcag
     4921 ggttccctgt ggaacggctt cgcgggtaat gaccaggttg atgccactgt tatctgtgga
     4981 caccaggacc tgcattgact gatcctgcca ttgagacggc agttgaatgt ggccttcatt
     5041 aaaagtgaac tgcataaaat actccctgat aatctgagtg aatcagttga tgaaaacaga
     5101 accgcccacg atggtgatgc catccttcga cagcctgatt accgtcgatt ccccgacctg
     5161 tagctgaatg ccatccggcg tcagcgcaat tttgctttcc tccacagcca gcaaaatttc
     5221 cttaatcgcc gtataagcct gtataccgtc gccttcgcca ccattgttta cagcgtcaac
     5281 atgcaccgaa ccggcaatcg tgcctctgtc ctgaaagaca tcctcagata aagacccttt
     5341 cagtactccg gtgatcttgt ggcctttttc caccgtaaga aggcggttac cttcctggat
     5401 cgtgaccgtc tggtcatttt ttacggtttc gcggtgatcg tgttttacgg tggtggtacg
     5461 gtcgttgagc acttcggtat ccatgttctt ctgcgcgtgg atgtagacct gttctctccc
     5521 tgtcgcatca tcaaacttca gctcattaaa tccgctgccc atgtacgttt tggagcggat
     5581 ggtcatctgc gtctttgttc ccggcaggct gccgggggtg cggttttcct ggtggtaggt
     5641 gcgccccata atgatcggct ggtccggatc gccgttgagg aagtctacaa tcacctcctg
     5701 acccacacgc ggtatcgcca ggtggccaaa accggtgcct gcccacgcct gtgccacacg
     5761 gatccagcac gaactgtctt ggtctgccgg gttataacgg tcccagttga acttcacccg
     5821 cacgcgacca tgttcgtcgc agaagatttc ctcgcctgcc ggtcctgtca cgacagcgct
     5881 ctgcgggccg tcgaccagcg gtttcagcaa cggctgtggt cgccatgttc tgtctgccgg
     5941 gatcaccgca aaatggttct ccagcgccgt ccccgctccc tgccgtcctg gcaccgcctg
     6001 tggctgttcg ccgtgcagtt cacttgccac cacctgccat tcccggttca ggttcgcctg
     6061 cggatgcccc gtcagcacaa ttcgccgtcc cggccatatc tccggcgagc ggctcattcc
     6121 ccgcgcggtt tctgcgttgt ttcgccagcc gtccatctgc cagcgggcaa agttctgccc
     6181 gtgggcgctc ttgaaacgtc cggggtagtc atacacttca tactgcgtgc gctggtagtc
     6241 ctggtgctgg ccttcctgtt caaaacgtcc ggcccagccg gggcgtttaa aggtgtagtc
     6301 tttggtcacc acggaagaag ggcggatttg tgcgctgtag cggaactggc tgatgcagag
     6361 ggtgctcacc tcggtacggg tgttcgggtt ccatgggatt tcaaaagatt cgggcagatg
     6421 gcggactgtg tcgcacagca ccaggctctg gtcggtactt ttgtaagcat gctcctcata
     6481 aaagaagatg ccttcctccg ccgccatccg gcacagaaaa tcgtaatcag tctcaccgta
     6541 ctggacacaa aactcacggg aaggatgcgg ctcgctgaac agcggactcc attcggtcac
     6601 cccgttttcc tgcaacatcg tgccgaggat gcttttgatg tcctcgttct ggaagatacg
     6661 gaagttctgg cgcagaccgg cacgccacag cggcgggtgc accttcatgc tgtacagcat
     6721 ctggtttttg tcgttctccc cgagttcaaa ccaggtcacc acgcctttca cccggcgctg
     6781 tacttcatcg ccctgccata tcgtcaggta ggccattttg tccagcacct gcgcaaattc
     6841 aagggagaga aactgctggc tgaccaggga gagatcgagg gaaaagagcg aagagagtga
     6901 ctggttcaga tgaaaggaaa ccaccgcaaa agcatccggc ggcaggccgt ccacttccag
     6961 tgtgaaacgt aatccggttg acatagcaac ctccagtaaa atgaataatg atccgtaccg
     7021 tacaactgcg gataatgtga taagcgggac gacttcagac gttattaggg aaggaacaga
     7081 aaatgctatg gttatgtgag aattttgttt aaatattact ttacagaaac gggtgtctgt
     7141 tcagtcaaaa aaataatcac aagctcaacc tcttgtttct tggcgttatt caggacaggt
     7201 acgcatat
//
LOCUS       DAPHJE010000174         7149 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPHJE010000174 DAPHJE010000000
VERSION     DAPHJE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.176"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..123
                     /locus_tag="RH222_004099"
     ncRNA           <1..123
                     /ncRNA_class="other"
                     /locus_tag="RH222_004099"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(307..984)
                     /gene="narI"
                     /locus_tag="RH222_004100"
     CDS             complement(307..984)
                     /gene="narI"
                     /locus_tag="RH222_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV3799030.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(984..1694)
                     /gene="narJ"
                     /locus_tag="RH222_004101"
     CDS             complement(984..1694)
                     /gene="narJ"
                     /locus_tag="RH222_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV3799031.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1691..3229)
                     /gene="narH"
                     /locus_tag="RH222_004102"
     CDS             complement(1691..3229)
                     /gene="narH"
                     /locus_tag="RH222_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV3799032.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3226..6969)
                     /locus_tag="RH222_004103"
     CDS             complement(3226..6969)
                     /locus_tag="RH222_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV3799033.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAAEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1587 a         2030 c         1870 g         1662 t
ORIGIN      
        1 atgcggggaa ggatgagaag cttcgactaa ggttcgactc gagcgccagc gagagagcgt
       61 tgccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct tcccccacca
      121 ccatcgcttc ctgaaataat caaaacctgt tactccgagc taattttgcc cgatggcacc
      181 acgtttaccg gacctacaat atcctcccct gtaggtcgga taagcgaagc gcatccgaca
      241 actcaccccg caccgtgccc ataaaaaaac cccgccgaag cagggtctat gtgaactaaa
      301 attcgcttag tggcgagccc gcaccagctg atacttacgt gtcagatact ccaccggtac
      361 gctccagatg tgcaccagac gcgagaacgg gaacagcagg aataacgtca tccccagcac
      421 caggtgcaga cggaagataa acgccacgcc atcaagatgt tgagaagcac caccgtggaa
      481 ggtcactacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgctg
      541 ggcggagaac ggaatggtca acaggcccag cgcgcactgg ataacgagca gcgacaggat
      601 caggatatcc gctccggtag tcgttgcacg cacgcgtggg ctgaacagac gacgcttaag
      661 caacagcacg ccgccaatca gacacagcac gccgctggca ccaccagcaa acatcgccat
      721 tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg tcaacatacc
      781 aaagaagtga ccgacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt
      841 catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc
      901 ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa
      961 gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc
     1021 ggcgcgacgg caccagcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct
     1081 ttgtcagcaa agaatttaac ctgctcttct tcccagacag catccagcgc ctgcggcgta
     1141 tcatcgcgcg cttcgtcggc aattttttcc gccactttgt cgctgtcgat agcggtattc
     1201 gccagtttca gcagcagatc aaacagcacg gcataacggc tttcacgctg ttgcagacgc
     1261 gcgctcagca atgccagaat cggcgcgata tctttcaaac cttccacggc ttcgctttgc
     1321 ggcagctgcg ccaggtactc cagatacagc ggcagatggt ccggcaattc gcggctgttt
     1381 aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg gccgcggtcg
     1441 cgggattcgc cgtgcacatg ttcaaacagc aacagtgacg tggcgcggcc acggtcgaac
     1501 agttcgctgt actgggcctg ggcatcgagc ggatccatcg ccgttaaatc gcgcaggaaa
     1561 atgcccagcg catgggcatc ctcttttggc agatttttcg acgcggcaat cgcctcaaac
     1621 atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc
     1681 acgagttcga tcatggatgc ggctccgttt tgctggtcac atcgatggca tcgatacgac
     1741 ggctgttgaa cagattgaat ttggtatctg aaccgtggca gccatcacca aaggtaaagc
     1801 cgcagccatt tttctccggg aaggcttccc gtgccagttc acgatgacta ctcggcacca
     1861 caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt
     1921 cggtcagacc gacctcttcc agcgcacggg tatcaacttt accgtcaacg gtttcagcac
     1981 gtttgtaatg acgcatcgcc agcatacgtt tcagcgcgcg cagtaccggt ttggtatcac
     2041 cggcggtcag cagattcgcc agatactgta ccggaatacg caggctttcg acgtctggca
     2101 gaatgccgtt gctacccagc tcacccgcgt ctgctgcaga ctgaatcgga gacagaggcg
     2161 gcacgtacca gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc
     2221 attccattgc cattttataa accggcgact gctgtgcagc ttcaataacg ctcagcggaa
     2281 taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacgtcca
     2341 gctgacgctg gtaaagatct ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg
     2401 catcgtataa cagcacgccg agataacgga tacgaccgac acaggtttct gagcacacgg
     2461 tcggctgacc cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct
     2521 tccagttgaa gtagattttt ttgtatgggc atccggtgat gcacatacgc cagccacggc
     2581 atttatcctg atcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg
     2641 ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca
     2701 tgaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt
     2761 tcgccagttt gtcaaactca ccacccaggt catcttccca gttcggccct ttttcgattt
     2821 tcgccatccg ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc
     2881 cttccggcgc ggtatgcagg ttctgataat caaaatcgaa cggctcgtaa taatcgtcga
     2941 tccccggcag atgcgggtta gcgaagattt tacccagcag catggcgcgg ttacccatgc
     3001 gcggctgcag tttgccattg attttacgga tccagccgcc tttgtatttt tcctggtttt
     3061 cccagtcagt cgggaagccc tgacccggct tggtttccac gttgttgaac cacgcgtatt
     3121 ccacgccttc acggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga
     3181 tgcacttatc gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc
     3241 ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac
     3301 aacaaactca tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg
     3361 ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat
     3421 accaccacgc tgttgggtaa tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg
     3481 gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg cagtcagagc
     3541 accgttgctg ttgaatactt caatccagtc gttatcggcg atacccagat ctttggcatc
     3601 ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc
     3661 gctgtaggtg gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc
     3721 cgggttgccg ttggatttct ggcctatcac ttctttcacc gaacgggtgt cgatcggcgg
     3781 acgataaacc agcaggcttt caccgaaatc acgcatccac tggtgatcct gatacagttg
     3841 ctgacgacca gagagcgtac gccatgggat cagctcgtga acgttggtgt aaccggcgtt
     3901 gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg ctggagataa ttttgcgcgg
     3961 ctgtgcctga atatcgcgga agcggatctt ctcgtcttct ttattcagcg ccagatgcgt
     4021 atggtcacga ccggtaaatt cgctcagggc agcccaggct ttcacggcta cctgaccgtt
     4081 ggtttccggt gccagtgtca ggatcatctc tgccgcatca attgcggtgt tcagcatcgg
     4141 ctggcctttc gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc
     4201 gctctgggtg ttccaggcaa tccctttacc gccattaccg attttctcca tcagcgggcc
     4261 gatagaggta aagcgttcgt aagtcgccgg ataatcgcgc tctacgacca taatgtgtgg
     4321 cgcggtttta cctgggatca ggtcgcactc gccttttttc cagtctttca catccagcgg
     4381 ctgcgccagt tcagcggcag agtcatgctg gataggcagc gtgacgatgt cggtttcttt
     4441 acccagatgg ccgacgcaca cttcggagaa tttcttcgcg atggctttgt agatttccca
     4501 gtcgcttttc gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg
     4561 catatccgaa gtattcatgt cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc
     4621 ggaatagaga caggtgctcg acagacggaa gtccagcgta accaccagat ccagcttgcc
     4681 ttccagacca ttgtcctgcc agtccacttc ttccggcttc acgccgccct gttgccccag
     4741 atctttaccc tggataccgt gctccgtccc cagcaggtac ttgagcataa actcatgacc
     4801 tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttgcgcg ggtggttttt
     4861 accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg atttcaccgt
     4921 atagtccacc ggattcatcc cggctttttc cgcttcggca gcgatagtca gcgggttagt
     4981 gcctaactgc ggtgcagacg gcagccagcc catgcgttcc gcacggacgt taaagtcgat
     5041 caggtgtccg gtataacggg atttatccgc catcggtgac agcaactctt ccgccgtgac
     5101 ggtttcataa cgccactggc tggagtggtt atagaaataa gaagtgctgt tcatgtgacg
     5161 cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc cagccggttt gtggacgcag
     5221 tttttcctga cctacatagt gcgcccagcc gcccccgctc tgaccgacac agccgcagaa
     5281 aatcagcatg ttgatcagac cgcgatagtt catgtccagg tgataccagt gattcagccc
     5341 cgcaccgacg ataatcatcg aacgaccgtg cgttttatca gcgttatcgg caaattcacg
     5401 ggcgatgcga ataatctggc tgcgagaaac gccggtaatc tgctcggccc aggccggggt
     5461 ataagctttc acatcgtcat agctggttgc acagttaacg tcgttcagac cacgttccag
     5521 accgtagttt gccagcgtca gatcataaac ggtggtcacc agggcggtgc tgccatcagc
     5581 cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt ccactttgtt
     5641 gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct acctcagcga tctcatcctg
     5701 gctacccagc aggctcagtt gcaactcggt ttcttcgcca gttttgccat cgcgctgctc
     5761 aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat
     5821 ttcgccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc
     5881 atcaaccaga tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc
     5941 cagcatcacc agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct
     6001 tgggttgtcg aggtggaatt cacgcagcat tacgtggccc atcgccagcg ccattgctgc
     6061 atcggtgccc tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc
     6121 tggtgtgacg gcaacagttt tggtgccttt gtaacgcact tcagtaaaga agtgagcatc
     6181 cggggtacgc gtctgcggca cgtttgagcc ccaggcgatg atgtagctgg agttgtacca
     6241 gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgcggagacg caggaggcag
     6301 gtcgcagtac cagtcgtaga agcttaagca agtaccgcca atcagcgaga gatagcgtgc
     6361 acccgatgcg taagaaacca tcgacattgc cggaattggc gagaaaccag caacacggtc
     6421 cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt tcacctcctg
     6481 ccaggaagaa cgaacaaaac caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc
     6541 ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc
     6601 acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt tggcactgta
     6661 aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcagatc
     6721 cggacgggta cgcggatagt cagtctgctg ggtttcccag gtgaccagac cgtttttgac
     6781 gtagattttc cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt
     6841 gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg
     6901 gccatgccca tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa
     6961 tttactcatc ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta
     7021 atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagagtga
     7081 tggggatgaa aaataaagta aattcctaag ataccccttt aggataatta acagccacag
     7141 cataacaga
//
LOCUS       DAPHJE010000175         7084 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPHJE010000175 DAPHJE010000000
VERSION     DAPHJE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.177"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(227..490)
                     /gene="cedA"
                     /locus_tag="RH222_004104"
     CDS             complement(227..490)
                     /gene="cedA"
                     /locus_tag="RH222_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV3799034.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            673..2934
                     /gene="katE"
                     /locus_tag="RH222_004105"
     CDS             673..2934
                     /gene="katE"
                     /locus_tag="RH222_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV3799035.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2981..3739)
                     /gene="chbG"
                     /locus_tag="RH222_004106"
     CDS             complement(2981..3739)
                     /gene="chbG"
                     /locus_tag="RH222_004106"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV3799036.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3752..5104)
                     /gene="celF"
                     /locus_tag="RH222_004107"
                     /gene_synonym="chbF"
     CDS             complement(3752..5104)
                     /gene="celF"
                     /locus_tag="RH222_004107"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV3799037.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5209..5979)
                     /gene="chbR"
                     /locus_tag="RH222_004108"
                     /pseudo
     CDS             complement(5209..5979)
                     /gene="chbR"
                     /locus_tag="RH222_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(5969..7069)
                     /gene="chbC"
                     /locus_tag="RH222_004109"
                     /pseudo
     CDS             complement(5969..7069)
                     /gene="chbC"
                     /locus_tag="RH222_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT         1954 a         1836 c         1577 g         1717 t
ORIGIN      
        1 agtttaagag cgacgccaca ggatgaacta tcaaaaatag ctcatcatga ttagcaaaac
       61 ttaaccattt taaaataaat aaacaattaa agaaaaaaga tcacttattt atagcaatag
      121 ctcgtcaaag gcagcttttt gttacaggtg gtttgaatga atgtagcaac gaaatgcaga
      181 atttcaggtc atgtaactcc cggcaaaacc gggaggtatg taatccttac tcagtcactt
      241 ccccttcctg gcggatctga tttgcccaac gttgggcaga ttcaggcaca gtaaacgccg
      301 gtgagcgcag aaatgactct cccatcagta caaacgcaac atatttgcca cgcagcatcc
      361 agacatcacg aaacgaatcc atctttatcg catgttctgg cggcgcgggt tccgtgcgtg
      421 ggacatagct aataatctgg cggttttgct ggcggagcgg tttcttcatt actggcttca
      481 ctaaacgcat attaaaaatt agaaaaactg tagtttagcc gatttagccc ctgtacgtcc
      541 cgctttgcgt gtatttcata acaccgtttc cagaatagtc tccgaagcgg gatctggctg
      601 gtggtctata gttagagagt tttttgacca aaacagcggc cctttcagta ataaattaag
      661 gagacgagtt caatgtcgca acataacgaa aagaacccac atcagcacca gtcaccacta
      721 cacgattcca gcgaagcgaa accggggatg gactcactgg cacctgagga cggatctcat
      781 cgtccagcgg ctgaaccaac accgccaggt gcacaaccta ccgccccagg gagcctgaaa
      841 gccccagata cgcgtaacga aaaacttaat tctctggaag acgtacgcaa aggcagtgaa
      901 aattatgcgc tgaccacaaa tcagggcgtg cgcatcgccg acgatcaaaa ctcactgcgt
      961 gccggtagcc gtggtccaac gctgctggaa gattttattc tgcgcgagaa aatcacccac
     1021 tttgaccatg agcgcatccc ggaacgtatt gttcatgcac gcggatcagc agctcacggt
     1081 tatttccagc catataaaag cttaagcgat atcaccaaag cggatttcct ctcagatccg
     1141 aacaaaatca ccccagtatt tgtacgtttc tctaccgttc agggtggcgc tggctctgcc
     1201 gataccgtgc gtgatatccg tggctttgcc accaagtttt atactgaaga gggtattttt
     1261 gaccttgttg gcaataacac gccaatcttc tttatccagg atgcgcataa attccccgat
     1321 tttgttcatg cggtaaaacc agaaccgcac tgggcaattc cacaagggca aagtgcccac
     1381 gatactttct gggattatgt ttctctgcaa cctgaaactc tgcacaacgt gatgtgggcg
     1441 atgtcggatc gcggcatccc ccgcagttac cgcaccatgg aaggcttcgg tattcacacc
     1501 ttccgcctga ttaatgccga agggaaggca acgtttgtac gtttccactg gaaaccactg
     1561 gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac tcaccggacg tgacccggac
     1621 ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg attttccgga atacgaactg
     1681 ggcttccagt tgattcctga agaagatgaa ttcaagttcg acttcgatct tctcgatcca
     1741 accaaactga tcccggaaga actggtgccc gttcagcgtg tcggcaaaat ggtgctcaat
     1801 cgcaatccgg ataacttctt tgccgaaaac gaacaagcag cattccatcc aggtcatatt
     1861 gttcctggtc tggacttcac caacgatccg ctgttgcagg gacgtttgtt ctcctatacc
     1921 gatacacaaa tcagtcgtct tggtgggccg aatttccatg agattccgat taaccgtccg
     1981 acctgccctt accacaactt ccagcgtgat ggcatgcatc gtatggggat cgacactaac
     2041 ccggcgaatt acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac accgccggga
     2101 ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg aaggaaataa agttcgtgag
     2161 cgcagtccat cgtttggcga atattattcc catccgcgtc tgttctggct aagtcagacg
     2221 ccattcgagc agcgccatat tgtcgatggt ttcagttttg agttaagcaa agtcgttcgt
     2281 ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac tctggcccag
     2341 gcggtggcga aaaatctcgg tatcgaactg actgacgacc agctgaatat caccccacct
     2401 ccggacgtca acggtctgaa aaaggatcca tccttaagtt tgtacgccat tcctgacggt
     2461 gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg aagtgagatc ggcagacctt
     2521 ctggccattc tcaaggccct gaaggccaaa ggcgttcatg ccaaactgct ctactcccga
     2581 atgggtgaag tgactgcgga tgacggtacg gtgttgccta tagccgctac ctttgccggt
     2641 gcgccttcgc tgacggtcga tgcggtcatt gtcccttgcg gcaatatcgc ggatatcgct
     2701 gacaacggcg atgccaacta ctacctgatg gaagcctaca aacaccttaa accgattgcg
     2761 ctggcgggag acgcgcgcaa gtttaaagca acaatcaaga tcgctgacca gggtgaagaa
     2821 gggattgtgg aagctgacag cgccgacggt agttttatgg atgaactgtt aacgctgatg
     2881 gcagcacacc gcgtgtggtc acgcattcct aagattgaca aaattccggc gtaaatatgg
     2941 ataaggtcac gtcccctggg gagtgacctt accaccttac ttacacatca agataactac
     3001 ccaaccgata accacgctca gcaattgcat atttcaacga cgctgatgtc agcacttcca
     3061 gctccgttaa ccgtgggaaa cagtaagcac tctggcgaat cgtattatcg acaaacgccg
     3121 gatggcacat gacttccagc gagcgttccc cgcgatggct ggaatcatca agcacttgca
     3181 ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct actgaatccg tgcgaactgc
     3241 gcagattagc cggtaaatca ccagcgttag atagcggctg acggtcgatg cgtaacgcaa
     3301 tcccctcttc agccgcaaac ctggcaacaa tagggaaaat ctgcgggaac atatgcacat
     3361 gatgatggct atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact
     3421 gactggcaag ctcctgagta atttcttcca gcggtaaagc atcttcttct gccaactgcc
     3481 agatccattt tcccagcaca ccatcgcggg ttaaccccgg catagctgtc agtggcttgc
     3541 ccatagtaag gacaaagtgc atccctatgg ccagactcgg ttcatcacga ctcaactgca
     3601 ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt cgatgtgaca atcccattgc
     3661 gacaggcctc gataatgccg tagttctgac ctttgcttaa gccaaaatca tcggcattaa
     3721 caatcagtaa gcgttccata atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc
     3781 agtcggcaaa atttggcagc catttctcgt gcgccagaat catctcgcgt gccagcagct
     3841 cagcatcgcg atcggaatgc accaacggac taaggtttag cgccagtaac acatcgttaa
     3901 attctccgct aagtgcggcg ttgctggcag caatctcgaa gcctttaatg gtgtgaatca
     3961 gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc
     4021 ccagcttaca ggtcatttct accgcccagt ctgccggaat attatcaata tgcccatgat
     4081 gcgggatatt aacgtaatgt tcagcttgct tgtcgttgta gatagcgttg atcacttcgc
     4141 acgctgcatc agagtaataa gccccaccgc gctgttccag ttctttcggc ttaactttca
     4201 actccggatt tttatacagc tcaaaaagtt gtttctcgac tttctgtact acctgtgctc
     4261 gtgcgccgcc tttgtagtat tcgcccattt caatagccag catctctttc tgcttgaagt
     4321 aatacagcag ataagaacat ggcagcagat ttaacgaacg aattaagccc tcactaaatg
     4381 gcagatcgaa aatatttttt acagaggacg cttttaactg ccctgacgcc acaccatcaa
     4441 gcaattcggc aaagcgcgac ttgccattta ccagcacatc cttaatgaac accatatggt
     4501 tgaggccgaa cagatcgata gataaatcat cgctgtcttt cagcatcaga acatcgcgaa
     4561 taaacatctt catgccgatc ggaatattac acacgccgat aaagcgttta aatccggtat
     4621 gacgataaac ggcttcagtg accattcccg ccgggttagt gaagttaatc acccatgcat
     4681 tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat caccggaatg gtacgcagac
     4741 ctttaaacag accgcccgcg ccgttggttt cctgaccaag ataaccatga cttaatggaa
     4801 tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg cagttgggta gtaacgaaat
     4861 cagcatcttt caatgcttcg cggcgatcca gcgttttata aagcttcatc gggacgccag
     4921 cgttatcaat catccgttgg cagagttcaa aaatgatatc cagtttctct ttaccatctt
     4981 cgacatccac cagccataat tcgctgaccg gcaattcgtg ataacgctta ataaatcctt
     5041 ccagtaactc cggggtatag ctgctcccgc caccaatagt gacgactttt aatttctggc
     5101 tcataatttc tcccttcagt acagaatact gatatctggc atatctgccc ccccggacat
     5161 aaataaccca gcaacaggac agatatgtga attgtcaggt ataacgactt actgattaaa
     5221 ttcagtcaat ttcttacgat aactcttagg cgtaaaggac gttaatttct taaacgtttt
     5281 aataaacaaa ctcgggctac tataaccggc ctcaaacgca atatccgtca cggaatagtt
     5341 ggtcatttcc agttgttttt tggcaaaatt aatacggatc tcattaataa tctgcattgg
     5401 tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt tggctgacaa
     5461 cgtcaccata ttctccagcg ccgattcact aaactgctct ttatcatgca tcttttctac
     5521 cgtacttttc agccactgcg gtacatcatc aatcacctgt tcttcgcggt aatgacgtaa
     5581 acggttaatg acataaaaag taaccatctc aacaaactct tccagccctg tttcgcggaa
     5641 attcaatgaa gaaatcactg tttccacata ggtgagaaaa gcgttattgg tccggtatac
     5701 ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa aaaagcgttt
     5761 actgatccca acgttcaata tacgcgtggc accaaactca taaaaacttt gatggtgcga
     5821 acctaacgga ataaaaacaa aatcgccccg ttccagtaac acgcgcttac cgttaatctc
     5881 ctggaaataa cgcccggtta ataccagagt aaattcataa tagtcgtgct gatgcagccc
     5941 gctgatactc tcagttttgt tatagataaa tcggttgtac cagtacaaac gggataaaca
     6001 tcaccgggtt catgataatt ggcagaccaa acagaatcgg ttcgttaatc tggaagatgc
     6061 cggacggcag cgccagtttt gccacctgac gataatcagc acggcgagat gcgataaaga
     6121 tagccaggat caggcctaaa gtcgcaccac tgccaccgag gaaaataaag gagtccagca
     6181 tcggcttggc ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat
     6241 attgctgata ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg
     6301 cggtcagcgc cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat
     6361 aggcccagcc caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga
     6421 tctgatggaa gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa
     6481 gaataataaa gccgggaatt aatgccgaga aggaacgcga tactgaagca ggtacgctgt
     6541 cgggcagttt aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca
     6601 ggccaataat aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat
     6661 aggcctcacc gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccgg
     6721 ccgccagcgc atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca
     6781 ttaaagacat tatccctaat gttccgttat atacgttgcc gccaatacct ttcagaccat
     6841 taagtgtttc aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa aacgacccct
     6901 ccccaaagct tagaaaaacg ttgttaatta atacaaacat ggcccccgca agggttaacg
     6961 gcattaagcg aataaagcca tttttgattg cattaacgtg tggctgcttt cctattttaa
     7021 ctgcaaaagg gaggagtacc ttttcaagcg atgcaataac attactcata gaaaaatacc
     7081 ctta
//
LOCUS       DAPHJE010000176         7081 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPHJE010000176 DAPHJE010000000
VERSION     DAPHJE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.178"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..1415
                     /gene="adeQ"
                     /locus_tag="RH222_004110"
                     /gene_synonym="yicO"
     CDS             81..1415
                     /gene="adeQ"
                     /locus_tag="RH222_004110"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV3799038.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSSVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1468..1920
                     /locus_tag="RH222_004111"
     CDS             1468..1920
                     /locus_tag="RH222_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV3799039.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2131..3321
                     /gene="nepI"
                     /locus_tag="RH222_004112"
     CDS             2131..3321
                     /gene="nepI"
                     /locus_tag="RH222_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV3799040.1"
                     /translation="MSEFIAENRGADAINRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3362..3655)
                     /locus_tag="RH222_004113"
     CDS             complement(3362..3655)
                     /locus_tag="RH222_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799041.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3877..4695
                     /gene="nlpA"
                     /locus_tag="RH222_004114"
     CDS             3877..4695
                     /gene="nlpA"
                     /locus_tag="RH222_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV3799042.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4699..5622)
                     /locus_tag="RH222_004115"
     CDS             complement(4699..5622)
                     /locus_tag="RH222_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3799043.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5733..6917)
                     /locus_tag="RH222_004116"
     CDS             complement(5733..6917)
                     /locus_tag="RH222_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV3799044.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARNSDKQGDRKLLILLCCLFGVLACTLFAWNS
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1777 a         1597 c         1785 g         1922 t
ORIGIN      
        1 atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat
       61 ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag
      121 ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga
      181 ttgcggggat gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc
      241 tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta
      301 tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg
      361 ggctgaacgc cttctttgcc ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg
      421 ggatgggcgc aatattctgg ggcgcagttg gactattttt gctcacgctg tttcgtatcc
      481 ggtactggat gatctccaac attcccttaa gtttacgtat tggtatcacc agcggaattg
      541 gattatttat cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca
      601 cgctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt
      661 ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg
      721 tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcagcgtc tattccattc
      781 cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac
      841 tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa
      901 ttggtgtaac tgataaagcg ggcttaatag atagtaacgg taaattcccc aatatgaata
      961 aggcgctgta tgttgatagc gtcagttcgg tggcgggggc gtttatcggc acctcgtctg
     1021 ttactgccta tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctgactg
     1081 cggttgtggt tggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg
     1141 ttcctcctta cgcaaccgcc ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc
     1201 tggcgcgcgt taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga
     1261 tgatgccctt tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca
     1321 tcatgaaagt atgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag
     1381 ctctgtttgc actgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc
     1441 atgagttctg attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta
     1501 gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc
     1561 agcgatcggc tgaacatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag
     1621 tcagctggtg acgaattttt acgttatttg catcgtccgg atgagtcgca cctgcaaaac
     1681 gccgcgcagg tgttgctcat ctggcaaatt gtcattgtcg atggtagcga acagaacctg
     1741 ctgcaatggc atcggatttt acaaaaagct cgcctcgccg cgccgattac cgacgctcag
     1801 gtcaggctgg cgctgggttt tctgcgcgaa accgaacctg aaatgcagga tattaatgct
     1861 tttcagatgc gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga
     1921 aaccgtttcg tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc
     1981 acgccgcatc cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac
     2041 accgtaaaag ttgcaaaaac gttaaatgcg tcacacattt caatgttaaa gtggtcggct
     2101 tttcccctga aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc
     2161 gcggatgcca tcaaccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc
     2221 tgtctgatta tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta
     2281 ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt
     2341 gccagtttgt ttattaccca gacaattcag gctactgacc gccgctacgt tgttattttg
     2401 tttgccgttt tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg
     2461 cttttaatcg gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg
     2521 tcgctgacca tgcgtctggt gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc
     2581 ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt
     2641 atcggttggc gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg
     2701 attatcaaat cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc
     2761 cgcttattac aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc
     2821 gggcagtttg ctttcttcac gtatattcgc ccggtgtata tgaacctggc gggattcggc
     2881 gtggatggct taacgctggt gctgttgagt tttggtatcg ccagctttat tggtacgtcg
     2941 ctttcgtcgt tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata
     3001 ctggctgtga gtgcgttggt actgacgttg tggggaagcg ataaaatcgt tgctaccggc
     3061 gtggcgatta tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc
     3121 acccgctcgc tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag
     3181 cttgctaata cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact
     3241 tcgccgttga tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag
     3301 gtgaaaatga agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt
     3361 cttacatatc cgggcattct accttaccca acgcttccca ataattgctc tgattattac
     3421 gttccatcgc cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta
     3481 acttttccca tgcttcatct gataatgacg cctgtggcgt gcaaatttta cgtaaatctt
     3541 gtaataccgt ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata
     3601 cgatgcctgg catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt
     3661 caacctttta caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca
     3721 tcatgctgta cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact
     3781 tttattcctt tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg
     3841 ctatagtcag gtaatcatta ataaaaggat aaaaaaatga aactgacaac acatcatcta
     3901 cggacagggg ccgcattatt gctggccgga attctgctgg caggttgcga ccagagtagc
     3961 agcgatgcaa aacacattaa agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa
     4021 gtcgctaaaa aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc
     4081 ggttcgttgc taccgaacga tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag
     4141 catcgaccct ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga
     4201 aatacgtttg tcttccctat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata
     4261 aaagaaggcg cgacagtggc gatccccaac gacccgacca accttggtcg cgcactttta
     4321 ctgttgcaaa aagagaagct aattactctg aaagagggaa aagggttatt acctaccgcg
     4381 ctggatataa ccgataatcc tcgtcatttg cagattatgg aactcgaggg ggcgcagctc
     4441 ccgcgcgtac tggatgatcc caaagttgat gtagcgatta tcagcaccac ttacattcag
     4501 cagacagggc tttctccggt gcacgacagc gtatttattg aagataagaa ttcgccgtat
     4561 gtgaatattt tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg
     4621 caatcttatc aatcacccga agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg
     4681 gtgcctggct ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg
     4741 acgggaatcc attgaaatca aaatcaccga tgacagaatc agcagcgttc ccagccagtc
     4801 cggtaatgtg aacgtgatcc ccagcaacag caaagagagt agcgcgctac ttaatggttc
     4861 tgcacagctc aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag
     4921 actaaatgtc agggacgtac caatgaccac caaataaaaa aacgccagaa tcaaactgcc
     4981 gttaacgaca aagtttgttc cttgtctggc ataaaaaggc aacagaatca gaccgccaat
     5041 cagcatactc cagccgacga ctggtaacgt gccatagcgg gcaattagcg ttgagggata
     5101 ggtggtatag aatgcagcag caaatgccga ggcaatgccc cagaacaacg cggcaggaga
     5161 gatcgataat gacgtcggat taccgtgtgt caccaataaa aaagtcccga ccagcgatgt
     5221 caaaatagcg cagaaaacca gaatgcccgg gcgcgattta cgcaccagtg agaaccaggc
     5281 gacgataatc gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc
     5341 gatggttagc aaaaaagtga gctgtacagt tagcgcgcca accacggaaa aaatcagcag
     5401 gctaatggca tctttatgat tgttaataat agaaaagatt ttatcgccat gaacaaatga
     5461 cagcgtcagt agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga
     5521 catctggctt tgctccatga tgtattgcgc gcagacccct gaacttcccc acaacacggc
     5581 ggcaatcaga acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg
     5641 ttttgtcgtt tatgtatgca ggcatgatag caaaaatggc gaggatggct ctgtttgcgc
     5701 cacgccgatt tttcttagcc cagactggtg agctaaatat ctttaataaa cagcaggcaa
     5761 atcatcgcaa tacccaacat ccctatcgcc agccagaaca acgcgtggta gctccagatt
     5821 tcaaccataa ttccgtcaac agagccggcg ataatccagc cgacgcgtga agtatttgca
     5881 tataacgttg tcgcagagcc tattttttca ggcatcaggt cctgaaaata aagcatgccg
     5941 ataccacaga gaataccaag gaagatggca tttagaattt gcaattccag ctcaactgcc
     6001 ggagtcgtcg ccatgagtac gctggcgtaa aaacacattc cactcacgat agcaatgagc
     6061 attaatagcc gcttgccaat acgtttcatg taatagcctg cgattaacat catcggaatt
     6121 tccagaccgg cagcgatacc aatcatttct ccagccagtt tatcggttag atgcagttca
     6181 tcaataataa atagcggcat atttatcatg tagagattat tcgccgccca catcattgaa
     6241 cagaccacaa aaagtagccg cgtatcccgc tttctatgga tggagggtaa aatttctacg
     6301 ggttgggtaa cgacaggaat atttctttgt attgatggca aaaacaacca gactatcagc
     6361 ccgcaaacaa caaatgcgat ggcagcggtg agatacatca ctttaaaact aaatcccatt
     6421 gccagttcat aagcgagcgg tggcccgata acccaggcaa gcgagatctg agcacgtaaa
     6481 aatgtactga acataaccgt ctcacggcct gttctgtcgg cgtgttcacg ggcgagggcg
     6541 aacatttgcg ggtttgcggt agaagcaaaa ctactcagaa gtacgcccgt tgagagcaga
     6601 atgaagtagt tgctattcca cgcaaaaagc gtacaggcca gcactccaaa taagcaacat
     6661 agcaggatca gtaatttacg gtcgccttgt ttatcggagt tccttgccag aaattgactg
     6721 accagaattc ccataatagc gctaccggtg aagaaaaaac ctaccattat aggacgggct
     6781 ttcagttcat ctgcgaggaa tatacttagg gtaggagtct gaagagcgcc cgcaataccc
     6841 gtcagaaagg cgacaagtaa aaatgccgcg gcagtgagat caagtatttt tgatggagtg
     6901 gtagccgttt tttgcattct tctgaatcac cgggcgaaaa aatataactg agttacaaat
     6961 aagtaccagt cgaatgagta ttgtattaag aatttttgag tatcgctgat acaaccagaa
     7021 agcaatgcta gcgcaatatt atagggccag caattgagat tattagcaaa agcatgttaa
     7081 g
//
LOCUS       DAPHJE010000177         7067 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPHJE010000177 DAPHJE010000000
VERSION     DAPHJE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.179"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(154..807)
                     /gene="ribB"
                     /locus_tag="RH222_004117"
     CDS             complement(154..807)
                     /gene="ribB"
                     /locus_tag="RH222_004117"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV3799045.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(911..1059)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1181..1471
                     /gene="ubiK"
                     /locus_tag="RH222_004118"
     CDS             1181..1471
                     /gene="ubiK"
                     /locus_tag="RH222_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV3799046.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            1754..2305
                     /locus_tag="RH222_004119"
     CDS             1754..2305
                     /locus_tag="RH222_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDV3799047.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            2365..4869
                     /locus_tag="RH222_004120"
     CDS             2365..4869
                     /locus_tag="RH222_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3799048.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGSSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            4885..5634
                     /locus_tag="RH222_004121"
     CDS             4885..5634
                     /locus_tag="RH222_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3799049.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            5636..6700
                     /locus_tag="RH222_004122"
     CDS             5636..6700
                     /locus_tag="RH222_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799050.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(6743..6943)
                     /gene="glgS"
                     /locus_tag="RH222_004123"
     CDS             complement(6743..6943)
                     /gene="glgS"
                     /locus_tag="RH222_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV3799051.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT         2162 a         1530 c         1443 g         1932 t
ORIGIN      
        1 ttttgatcat aatgaaaaag aatgcctgat attaatgaaa ttttctcata gaaagagaag
       61 cggaattaac atcttgcata gcagggcaaa ataaaaaccg aagccagcta caacttcggt
      121 ttcttgatta aggcagtaat taagcagcgg ttttcagctg gctttacgct catgtgcctg
      181 acggtatgcc accaggtctt caatagtcac gagcgccata ttgtgtttat tggcaaactc
      241 aatgcactct ggtgcacgcg ccatcgtgcc atcgtcatta gtcaactcac acagcacacc
      301 agccggttta aagcctgcca gcgtcatcaa atcaatagtc gcttcagtat gaccgccacg
      361 cgtcagtaca ccacctgcct gcgcgcgaag tgggaaaacg tggcccgggc gattcagatc
      421 agaaggtttt gcgccatcgg caatcgctgc gcgaacggtc gtaattcgat cagcggcaga
      481 aacaccggta gtcacacctt cagccgcttc aatggtcacg gtaaaaccgg tgccataggc
      541 gctggtgtta ttttctacca tcattggcag atcaagttgt ttacggcgat cttcagtgat
      601 gcacaggcaa acaataccgc taccgtggcg aatggtcagc gccatctgct caacggtcat
      661 ggtttctgcc gggaagatca tatcgccttc gttttcacgg tcttcatcat caagcaccat
      721 tacaccgcgt ccttcacgca gcgcagccag tgcattttca acacgttcga aaggcgtacc
      781 aaaagaggaa agtagcgtct gattcatggt aaaaaaacct cactaaaatt atggttacca
      841 gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc
      901 ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg
      961 gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt
     1021 accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat aacgctaatg
     1081 attagcggca gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac
     1141 tacaatggca ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat
     1201 tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga
     1261 agatgtggag aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt
     1321 aagccgtgaa gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc
     1381 gctgcttgaa caaagaatga gcgaactgga aaatcgcagt actgagatta aaaagcagcc
     1441 agatccagag actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat
     1501 gaattaatct tctaatttac agtgctttct attaccttat cgcttaattc cattcagaat
     1561 atcatcacaa aaatgaatga gtgcaattga aaatataatt gttatgtatt ttttaagttt
     1621 tacttaatcc atatttagat aaactaattt cataaaaccc gaaaagtaaa atataatatt
     1681 tgaatgatta gcaatcctta aataacagtt actaatcatt cattttctac ttatatttat
     1741 ataaaggaat attatgtctg cttttaaaaa gtcacttctt gttgctggcg tagcaatgat
     1801 tctttccaat aacgtatttg ctgacgaagg tcacggcatt gttaaattta aaggtgaagt
     1861 tatctctgct ccatgttcaa ttaaaccagg tgacgaagac ttaactgtaa atctgggtga
     1921 agtagcagat accgttctga aaagcgacca gaaatctttg gctgagcctt tcactattca
     1981 cctgcaagac tgcatgttga gccagggtgg aaccacttat tcaaaagcga aggttacttt
     2041 caccacagcg aatactatga cgggtcagac tgacctgctg aaaaacacca aagaaactga
     2101 aatcggtggt gccacaggcg ttggcgtacg tattctggat agccagagcg gcgaagtgac
     2161 tttaggtact ccggtagtaa ttacattcaa caatactaac agctatcagg aactgaactt
     2221 caaagctcgt atggaatccc caagcaaaga tgctaccccg ggcaacgttt acgctcaggc
     2281 tgactataag attgcttacg agtaatcaca ttaggccggg ttcgcccggc cacatcatta
     2341 acggattaat gataagtgga acagatgtat aaaaaattaa aattaaccac aataagcgaa
     2401 ttgattaaaa atatttattg ttcattatcc gttctcatca ttggttgtgc gtcagcttat
     2461 gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc gtgaaaacgt taacctttcc
     2521 caatttgaaa ctgatggcca attacccgta ggtaaatact cattaaacgc tcttattaat
     2581 aataaaagga cgccaatcca tctggacctg caatgggtat taattgataa ccaaactgca
     2641 gtttgtctga ctccagagca attaacatta ttaggcttta ctgatgagat tattgaagag
     2701 gctcagcaaa acctgatcga tggttgttac cctatcgaaa aagaaaaaca aattacaact
     2761 tatctcgata aagggaaaat gcaattatcc atatctgcac ctcaggcatg gttaaaatac
     2821 aaagatgcaa actggacgcc tcctgaactt tgggatcatg gtattgctgg ggcatttctt
     2881 gactacaatt tatatgcctc tcattatgca ccacatcagg gcgataattc acaaaatata
     2941 agttcctatg ggcaggctgg ggttaatctt ggggcctggc gcctgcgtac tgattaccag
     3001 tacgatcagt catttaacaa tggcaaaagc caggcgaaca acctggattt tccgcgtatt
     3061 tatttgtttc gcccaatccc agcaattaat gcaaaactaa ctataggtca atacgatact
     3121 gaatcctcta ttttcgactc tttccatttt tctggcgttt cgttgaaaag cgatgagaat
     3181 atgttgccgc ctgatctacg tggttacgca ccgcaaatca cgggtgttgc acaaacgaat
     3241 gcaaaggtca ctgtctcaca gaacaaccgt attatttatc aagaaaatgt tcctcctggc
     3301 ccatttgcta ttactaattt attcaataca ttacaggggc aactggacgt caaggttgaa
     3361 gaagaggacg ggcaagtcac gcaatggcaa gttgcatcta atagtattcc ttatctgacg
     3421 cgtaaagggc agattcgcta caccactgct atgggtaaac cgaccagcgt tggtggtgat
     3481 tccttacaac aacccttctt ctggactggt gaattctcat ggggttggct gaacaatgta
     3541 tccctgtatg gtagttcagt tttaacaaac cgtgattatc aatctctggc tgccggcgtt
     3601 ggctttaacc ttaactcatt gggttcatta tcttttgatg tcacacgatc tgatgctcag
     3661 ttgcataatc aggataaaga aacgggttat agctaccgcg ctaactattc aaaacgtttt
     3721 gaatctaccg gtagccagct cactttcgct ggttaccgtt tctctgataa aaactttgtg
     3781 acaatgaatg aatatatcaa tgacactaac cattacacga attatcagaa tgaaaaagag
     3841 agttatattg tcacgtttaa ccagtatctt gaatcattaa ggttaaatac atacgtaagt
     3901 ttggctcgta atacttactg ggacgccagc agtaatgtga attattcatt atcacttagc
     3961 cgcgattttg atatcgggcc attaaaaaac gtctccactt ctctaacatt tagccgaata
     4021 aactgggaag aagacaacca ggatcaactg tacctaaata tttcgattcc ctggggaacc
     4081 agtagaacat tgagctatgg tatgcaacga aatcaggata acaagatttc gcatactgct
     4141 tcgtggtatg actcttccga tcgaaataat tcctggagcg tttctgcttc aggcgacaat
     4201 gatgaattca aagatatgaa agcgtcacta cgcgccagtt atcagcataa taccgagaac
     4261 ggtcgtctct atctctccgg gacatcacag cgagacagtt attattctct gaatgccagt
     4321 tggaatggtt cattcacggc gactcgtcac ggagccgctt tccatgacta tagtggtagt
     4381 gctgactcgc gttttatgat cgacgcagat ggtgcagaag atattccgtt gaacaataaa
     4441 cgcgcggtaa ctaatcggta tggcatcgga gttattccat cagtcagcag ttacataaca
     4501 acatcattaa gcgttgatac ccgaaatctt ccagaaaatg tggatatcga aaactcggtt
     4561 atcaccacca ccttaaccga aggtgctatt ggctacgcca aacttgatac ccgcaagggc
     4621 tatcaaatca tgggggttat tcgcctggca gatggtagtc acccaccact ggggattagc
     4681 gtaaaagata aaaccagcca taaagaatta ggactggttg ctgatggcgg ctttgtatac
     4741 ctcaacggca ttcaggatga cagcaaactt actttacgct ggggtgataa atcctgtttt
     4801 attcaaccac ccaatagcag caacttaacc accggaacgg ttattttacc gtgtattagc
     4861 caaaattaat tcaggatgaa actaatgcgc tacttaaaca caaagaatct cattgcagca
     4921 ggagtattac tcgcctgcat gagcagcatt gcctggggtg ccatcatccc ggaccgtacc
     4981 cgtatcataa tgaatgagtc agataaaggg gaagcattaa aacttactaa tcaaagcaaa
     5041 aatctaccct atctggcgca aacatggata gaagatacga aaggcaataa atcacgtgac
     5101 tttattgtca cggttccccc tatggtacgt ttaaatccca gtgagcaaat tcaaataaga
     5161 atgataagtc aggaaaaaat tgctcaactg cctaatgaca gagaaacgct cttctatttt
     5221 aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg tgatgcaagt cactatgcag
     5281 cacgcattga aactattctg gcgaccaaaa gctattgaat tagaagatga tggggtaatg
     5341 tcttacgaaa aagttgaaat catccgtaga aatgatggtt cgatccgctt taacaataag
     5401 atgccttatc acgttacgct gggttatatc ggaacaaacg gaatcacaat gttgccacaa
     5461 acgcaaagtt taatggtaac cccttatagc catgcagata cgcagtttaa aaatgttccg
     5521 tcagcttttc aggttggtta tatcaatgat tttggtggat taagttttta tgaaataaac
     5581 tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca aacgagataa ataatatgcg
     5641 ctacttatta attgtaatta tatttattat aggttttagt gcattacctg tatgggcaat
     5701 ggactgctat gctgaacatg aagggggaaa taccgtagtc attggttacg taccaagaat
     5761 ttctatcccc agtaatggta aaaaaggtga taagatctgg caaagcagtg agtattttat
     5821 gaatgtcttc tgtaataatg cattacctgc cccatcacct ggagaagaat acccatcagc
     5881 ctggactaat ataatgatgt ttttaccagg cggtcaggac ttttataatc agaattccta
     5941 tatttttggc gtaacataca atggagttga ttatgattcg actgccccta atgctatcgc
     6001 agcaccagaa tgcattgata taaaaggagc gggtaccttt aataatcact ataaaaaccc
     6061 cgctgtctgt agtggaggtc ctgagcctca attatcagta acctttccag ctcgcgtaca
     6121 actctatatt aaattagcaa aaaatgccaa tagagttaat aaaaacctgg tgttacctga
     6181 tgaatatatc gctctagagt ttaaaggtat gagtggcgca ggagctatag aagtagacaa
     6241 aaatttgacc ttcaggattc gtggattaaa taatattcat gtccttgact gctttgttaa
     6301 tgtcgcactg gaaccagctg atggcgttgt tgactttggt aaaataaatt cccgaacaat
     6361 taaaaatacc agcgttagtg agacgtttag tgtagtcatg acaaaagatc ctggtgcggc
     6421 ctgtactgag caattcaata ttttagggag ctttttcact acggatattt tgagtgatta
     6481 tagccatctg gatatgggta atggactgct attgaagata tttcataaag atggaacagc
     6541 aacggaattt aaccgcttct cacaatttgc ttctttttca tcgtctagtg caccttcggt
     6601 caccgcacca ttcagggcag aacttagtgc gaatccggca gagactgttg ttgagggacc
     6661 gtttagtaaa gacgtcatcc tcaaaatcac ctataactag catctaatac aaacactaaa
     6721 acgggccatc aggcccgtta tatcagtgct ctaactccag ctctcttgcc tgcatcatct
     6781 gttgacaata ccaggcataa cgtgcgcaaa accaggttct atgttcttca tccatgttac
     6841 cagtaacggc aagaatatcg accgggcgac cttctgcgtg cattctggca aaactacgcg
     6901 ccaggaaatc gaaattattt aaagaattaa gactatgatc cataagcaca tcctccggtt
     6961 atcgcatacc ttttaaggcc atatgctttt tctgatttca gtattcccca atcagtgtag
     7021 tgcctttaaa cataacgtgc gtaaatatat tgtccccgat cacactt
//
LOCUS       DAPHJE010000178         7064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPHJE010000178 DAPHJE010000000
VERSION     DAPHJE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.180"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..199
                     /locus_tag="RH222_004124"
     CDS             <1..199
                     /locus_tag="RH222_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV3799052.1"
                     /translation="ESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLF
                     KKIAVKNRTQAVSWANDNLRR"
     gene            204..593
                     /gene="csgE"
                     /locus_tag="RH222_004125"
     CDS             204..593
                     /gene="csgE"
                     /locus_tag="RH222_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV3799053.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            618..1034
                     /gene="csgF"
                     /locus_tag="RH222_004126"
     CDS             618..1034
                     /gene="csgF"
                     /locus_tag="RH222_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV3799054.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1061..1894
                     /gene="csgG"
                     /locus_tag="RH222_004127"
     CDS             1061..1894
                     /gene="csgG"
                     /locus_tag="RH222_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV3799055.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(1958..2449)
                     /locus_tag="RH222_004128"
     CDS             complement(1958..2449)
                     /locus_tag="RH222_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV3799056.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2551..3105)
                     /gene="ycdY"
                     /locus_tag="RH222_004129"
     CDS             complement(2551..3105)
                     /gene="ycdY"
                     /locus_tag="RH222_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV3799057.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3129..3866)
                     /gene="ycdX"
                     /locus_tag="RH222_004130"
     CDS             complement(3129..3866)
                     /gene="ycdX"
                     /locus_tag="RH222_004130"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV3799058.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3921..4859)
                     /gene="ghrA"
                     /locus_tag="RH222_004131"
     CDS             complement(3921..4859)
                     /gene="ghrA"
                     /locus_tag="RH222_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV3799059.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGACLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            5093..5180
                     /locus_tag="RH222_004132"
     tRNA            5093..5180
                     /locus_tag="RH222_004132"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5127..5129,aa:Ser,seq:gga)
     gene            5225..5361
                     /locus_tag="RH222_004133"
     ncRNA           5225..5361
                     /ncRNA_class="other"
                     /locus_tag="RH222_004133"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5554..6540)
                     /locus_tag="RH222_004134"
     CDS             complement(5554..6540)
                     /locus_tag="RH222_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799060.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
     gene            complement(6537..>7064)
                     /locus_tag="RH222_004135"
     CDS             complement(6537..>7064)
                     /locus_tag="RH222_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV3799061.1"
                     /translation="AVKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQASLNEF
                     ATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEV
                     APSIIKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQR
                     VMHCRKAFSDAKVFK"
BASE COUNT         1979 a         1768 c         1601 g         1716 t
ORIGIN      
        1 ggaatcagcc ctccttactc atcgggaaaa agagatcctg aataagctgc gtatcggcgc
       61 gtctaataac gagatcgctc gttcgttgtt catcagcgaa aatacggtta aaacgcatct
      121 ttataatctt ttcaagaaga tagccgtaaa aaaccggaca caagcggttt cctgggcaaa
      181 cgataacctc aggcgataaa gccatgaaac gttatttacg ctggattgtg gcggcagaat
      241 ttctgttcgc cgcagggaat cttcacgccg ttgaggtaga agtcccggga ttgttaactg
      301 accatactgt ttcatctatt ggccatgatt tttaccgagc ctttagtgat aaatgggaaa
      361 gtgactatac gggtaactta acgattaatg aaaggcccag tgcacgatgg ggaagctgga
      421 tcactataac ggtcaatcag gacgttattt tccagacttt tttatttccg ttgaaaagag
      481 acttcgagaa aactgtcgtc tttgcactga ttcaaactga agaagcacta aatcgtcgcc
      541 agataaatca ggcgttatta agtacgggcg atttggcgca tgatgaattc taaataaaaa
      601 attgttcgga ggctgcaatg cgtgtcaaac atgcagtagt tctactcatg cttatttcgc
      661 cattaagttg ggctggaacc atgactttcc agttccgtaa tccaaacttt ggtggtaacc
      721 caaataatgg cgctttttta ttaaatagcg ctcaggccca aaactcttat aaagatccga
      781 gctataacga tgactttggt attgaaacac cctcagcgtt agataacttt actcaggcca
      841 tccagtcaca aattttaggt gggctactgt cgaatattaa taccggtaaa ccgggccgca
      901 tggtgaccaa cgattatatt gttgatattg ctaaccgcga tggtcaattg cagttgaacg
      961 tgacagatcg taaaaccgga caaacctcga ccatccaggt ttcgggttta caaaataact
     1021 caaccgattt ttaagcccca gcttcataag gaaaataatc atgcagcgct tatttctttt
     1081 ggttgccgtc atgttactga gcggatgctt aaccgccccg cctaaagaag ccgccagacc
     1141 gacattaatg cctcgagctc agagctacaa agatttgacc catctgccag cgccgacggg
     1201 taagatcttt gtttcggtat acaacattca ggacgaaacc gggcaattta aaccctaccc
     1261 ggcaagtaac ttctccactg ctgttccgca aagcgccacg gcaatgctgg tcacggcatt
     1321 gaaagattct cgctggttta taccgctgga gcgccagggc ttacaaaacc tgcttaacga
     1381 gcgcaagatt attcgtgcgg cacaagaaaa cggcacggtt gccattaata accgaatccc
     1441 gctgcaatct ttaacggcgg caaatattat ggttgaaggt tcgattatcg gttatgaaag
     1501 caacgttaaa tcgggcggtg ttggggcaag atattttggg atcggtgcag acacgcaata
     1561 ccagctcgat cagattgccg tgaacctgcg cgtcgtcaat gtgagtaccg gtgagatcct
     1621 ctcctcggtg aacaccagta agacgatact ttcctatgaa gttcaggccg gggttttccg
     1681 ctttattgac taccagcgct tgctggaagg ggaagtgggt tacacctcaa acgaacccgt
     1741 tatgctgtgt ctgatgtcgg ctatcgaaac aggggtcatt ttcctgatta atgatggtat
     1801 cgaccgtggt ctgtgggatt tgcagaacaa agcagaacgg cagaacgaca ttctggtgaa
     1861 ataccgccat atgtcggttc caccggaatc ctgacagata aaaaagccgg aattatcatc
     1921 cggcatttta ctgtttaagc gaaagcgtga gtgcccctca cgctttattt ttgatttcct
     1981 gctcacggtg agccgtcttt gcactacgcg ccgccagcca caggccgctg tttttcattg
     2041 cgtaaccaaa taccagccca agcgccagcg aaggtaacac cagtttccag tcaccctgcc
     2101 cggcaaacgt cgcgcatgcg cctataaacg tccccggaac aaatgaaagc agcagctgtt
     2161 tggcctgaat acacatcaga aacgccacaa taccggttat gacataaccg agaatttcca
     2221 gatgtggtgc cagtgcacta ccgtaaataa tgaccatcgc ccacaccacg ccactaagca
     2281 gggttgcagc ggagatcgcc agccctttca gcccaccttg cgggcaggca aagtaggccg
     2341 tacagcccag gaagcccgcc cagcttagta agcccaggga aacagccacc catccccaga
     2401 taccggagag aatgcctgtt gtgattgcaa tagagagaag tatgttcatg ccgcgcatga
     2461 tagcagaaaa cgcggccatg aatgagatcg agcacacatt ttatgcaaca acaaattatc
     2521 ttttgcagtt atgagtgacg atgatcacat ttactcttca gaatcttctt ccagctcgtc
     2581 ccacattgca ctaatggcat cacgggttag cggtgccatg gtgcgccaga aaggcgtggt
     2641 tgcatgggcc tccactttgc cgaggaacgc accacaccag ggtaacagat actcactgaa
     2701 cagtgtttcc agtgcttcgc tctcatcttc cgttgactga tcttccagcc aggaagccgc
     2761 aagcagcaat gtgccgatgt gatcggctgg cgtatccgcc agtggcatcc ctcgttcgga
     2821 aagaaaagcg cgcacttccg cttccgttgc gtcctcaacc catgcgctac gatatggcgg
     2881 cacggcacat tcatcgccga taaacaacgc attgtaatcg gcagagactt gcgccatatc
     2941 acagcttttc tgtaaacgcg tcagtaactc atcctgctcc agtggccagt tcgcagccag
     3001 tttcccctca cgaatcaggg taaacagcgg caccagtaaa ggatcttgcg gttggcggta
     3061 atagagcgaa cccagcacac gacagaggat agaaaactcg ttcattaata tatttccatt
     3121 actagtaatt aaaggtctgc aaattccgca atcggtgcca taccgcgaga ttcaaggaag
     3181 ttcagtaagc ggcgcggaga aacattcaaa atgcgctctg gcggaaaatc taccgcgtcg
     3241 aggattttaa gacactcttc aaattccccc atggtaaacg cggtgtgaga atccgagcct
     3301 aatgccaccc aacctccagc atcacgtacc gctgcggcta ccgcacgaca gttgtcttca
     3361 ctgcccttac gtgagtgtaa aaatgaagaa ttattgattt ccagcgccac ctgatgtttc
     3421 gcggctgctt cagcaacggc tttcacatct atttcatatt tgggatttcc gggatggctt
     3481 attatatgca cattgccgct ggcgatagtg gcgatcatcg cttgtgtatt ggtcgctttg
     3541 tcatgtggtg caaaaaccgg ctcatgaaaa ccggcaataa tcagatccag cgagtcaaac
     3601 attttaccgc tgcaatcaat ttcaccatca acatttttaa tgttagcttc gatgccgcgc
     3661 aggatcccta ccccatcaac cactcgcggc cagatacgca tgttaatgaa gtgccagtga
     3721 tgcggagcat cttccatatc cgggccatga tcggtgatcg caaaaagttt aatgcccttt
     3781 tgtttggcct gggcaatgta atcactcaat gtgctatatg catgtgtgct ggcaacggta
     3841 tgcatatgaa ggtcgacggg atacataact ctctcctgta ttcaattccc gccaaggata
     3901 gcaggaatcc tgatgcttta ttagtagccg cgtgcgcggt cgacttgccc gcagaccttc
     3961 tccccttttt cgagctgggc aatagtgcga gaaatgtact ccacagcttc agcgggacgg
     4021 gtaatcgcgg cgacatgtgg tgttatcgtc acgcgtggat gttgccagag cggactttca
     4081 ggcggtaagg gttcacgatt aaaaacatcc aagcatgcgc ctttaacttt cccgctatcc
     4141 agcgccgcga gcaggtcatc ttccacaaca tgaacaccac gcgccaggtt gaggagatac
     4201 gcgccatccg gtaatttttc gagtaattgt tgattaataa tgccgacggt ttcaggggta
     4261 ttcggtaaca aattaatcaa tacccgacat tggctcagaa atgcagacag ttcttcccgt
     4321 ccggcaaagc tttgcacgcc aggccacgat ttacgggttc gactccagca acgcagcgga
     4381 aagcgccagg tttgcagact ctgagcaact ttactgccca gtacgcctgc gcccaaaatg
     4441 ccgatggtaa aatcttcccg atgatattcc ggcagcggtt gccaatgcga actattttgc
     4501 tggatgcgat aatcgtcaaa acgtcgaaac caatgcagca cctgactgac agcatattcc
     4561 tgcatttgct cgcccatacc ggtatcttcc aggcgaaaaa gtggaacaga agggttcagc
     4621 atttcagggt gtgcctgtag cttgctcaaa atagaatcaa caccggcccc gagtgcgaac
     4681 accgctttaa gatcgcgccc tgccagcatt tcaacaggag gatgccagac taaagcataa
     4741 tcagcagagt cattatctcc gcttttccac gctctgactc ttgcctgagg aatagctttg
     4801 cgcagtgcct caatccacca ttgggtatcg aacgttgggt gataaaagat gatatccata
     4861 ctgactcccg aaaagcgttg tgcgaaattt attcgcactt atcgttatga tctacaaagg
     4921 ccaccagcat aacaaatccg tggtcggtgg caaaaaaagc agatttcgct tattaaaacc
     4981 acacattgat tgaagtttga ataaacgcgc gattttttca aaaagtttgt tgacctcagg
     5041 tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat ttggtgaggt
     5101 gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta tcgggggttc
     5161 gaatcccccc ctcaccgcca tatttaaaga agagctcgta cgaaagtacg ggcttttttt
     5221 tcgtatattg cacacaccgg ggggatgaga agccccgacc ggggttcgac aactggcgac
     5281 agccagttgg acagaccgtg aacgcagtga gcgggctgcc cgcagggcga gcgaagcgag
     5341 tcaatccccc cctcaccgcc atatttaaag aagagcccgt acgaaagtac gggctttttt
     5401 ttcgtatatt gcacacaccg ggtgcccccg cccacgtctg ttagggcgag ggaaaattgt
     5461 gcacatcaac tgtctggtta ctcagtcaac aatcaaaatg cgcaagatag tagagaacat
     5521 atatgcgtta tctggtcact tcctgtaact tcctcactta ttaaaatgcc cccaacggaa
     5581 atacatgtca catgccatca taaaatcgtt atgccagagg catcgacaat ggaaaaaata
     5641 gtaaattaag cacggtacat agcagagtaa aaaataggct tcaaaccaaa tccgccattc
     5701 cacaagcgtt gccaataccg acaccagcgt aatccacatc atttcaataa ccagcaatat
     5761 gaataaccga agccaaagcg gcttggcaaa aaaggtcgac aacgcatttt cactggagaa
     5821 aaacagatcg atttcctgca aaaaaagcct gcccagaaaa gcgtaccgct tggagaagcg
     5881 cagaaaaaac agattaatcg ccaacactac gccacaaaga atgagcaatg caattatcga
     5941 tttaaacgtt aaccactgcc cctctgtcac aaaagaatca ctgttacgta acgggctgat
     6001 agtaagcagg ttaacaaccg ttgagatgca caccgaaagc agcacatagc gagtcagact
     6061 tttatatttt acttttttcg ctactgaaat agcgacctgc ttgacataac tggtgcggat
     6121 catccgaaac agcagaaaga tgaccaacgg cccaagtaac gaaatcagga acagcgcata
     6181 taacgagggt agaagctggt atagccccca actcattgca ctcaacatga ttaacgacag
     6241 aaatcctgcc accagaggtt gtgtaagaaa taattctatt ttgtggttct gctttttctt
     6301 aaaccacgaa aaatcgatat tcttcgtgac gcatttataa gcccagtacg ccttgagatc
     6361 aaaaataaaa ttatacagca ccagggccag aatcccacta acgacaccaa tatcctctga
     6421 tggaaaagta aatcctgcat ttttccaata aaagcctatc agaataagtc caatagcata
     6481 aatgtatatc agatttcgtt tatcctgaaa gcgaataaaa gttaaataat caggaatcat
     6541 ttgaatacct ttgcatcact aaatgcttta cgacaatgca taactcgttg ctgatccacg
     6601 ccagcaaaat catttaacat attccccaaa tagggcttat tacttatttc aaggaaagct
     6661 accggggttg gcatattcag aaatgtatcg agagattcag tccatttcac cgtatgggtt
     6721 aagtgtaatg agagattttc tttgataatt gacggagcga cctcgctttt tgctgttacg
     6781 ttcatcagaa cctgatgctc tggcgaagcg atatccagtc cggcaagata atcgcgcatt
     6841 gcctgaacgc cgtcttccat caaacgcgtg tgccaggcac cgctcacacc gagtttaacc
     6901 ggttcgtatc cagcggccat cagcagcgtg gcaaattcat tcaacgaggc ctgcgttccc
     6961 ccaataacct gctggcgcgg cgtgttatca cagctaatat ccagcgcaat gcctgattcc
     7021 gtaatcatcg tctgcagttg ttcgcggttg atgcctttaa ccgc
//
LOCUS       DAPHJE010000179         6932 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPHJE010000179 DAPHJE010000000
VERSION     DAPHJE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.181"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="RH222_004136"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="RH222_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV3799062.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="RH222_004137"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="RH222_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV3799063.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="RH222_004138"
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="RH222_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV3799064.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="RH222_004139"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="RH222_004139"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV3799065.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="RH222_004140"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="RH222_004140"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV3799066.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="RH222_004141"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="RH222_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV3799067.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1755 a         1628 c         1808 g         1741 t
ORIGIN      
        1 taatgagcac agtgataatt aatgacgaaa tgaatgatgt gcattttcca catctttgag
       61 ttgcggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag ttaaaaggta
      361 atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca
      541 accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 cagcctggct gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg
      721 ctctgccatc gtcgcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac
      841 taaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggcgattac cgccaacgaa ctggataccg cccgccgccg
      961 tactacggga tctatggatg cgctgaaagc ggccggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gcattggcat
     1201 taacggtgtg gatgcggtga gcgaactgtc taaagcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt
     1321 agcaaaagac gttgaaccgc caaaatttac cgaagttacc gacgtggtac tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggcgtg gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaagcgc tggcgcgtaa
     1981 cgccaaaatt atcgcctttg atgagccaac cagctccctc tctgcccgtg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtaatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccattact gtctttaaag atggacgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt
     2221 cgggcgcgac attggcgata tctacggctg gcaaccgcgt agttatggcg aggagcgcct
     2281 acgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggtgaaatt gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg
     2401 catgtttggc gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 taaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgcggag caactgatca tgaatctctc
     2701 aggcggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggcgct aagcacgaaa tatataacgt
     2821 aatttatgcg ctggcggcgc agggcgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggctgttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg gtctggcgca
     3061 cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgctct ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cttgtggcat gttgttctgc
     3241 ctcgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 acggcggtgg ttattaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgttg
     3361 ctgggcgttc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacga cattggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc caactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgctgctg
     3601 aataaaacca cctttggtcg taacaccctg gcgattggcg ggaacgaaga ggccgcgcgt
     3661 ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc aggcctggta
     3721 tcagcgatag ccggaattat tctggcttca cgtatgacca gtgggcagcc aatgacgtcg
     3781 attggttatg agctgattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg cttaatcctg
     3961 ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttctgcaac aatttagcgt tttttcccac catagccaac cgccataacg gttggctgtt
     4081 cttcgttgca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaac acaataagaa aagagaagga ggagaaccgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatccgcga aatatgcctg gttttttgat cttgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aggtcgtcgt gcctgacaat attctgcaag gactacagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct
     4381 tgacgcactg gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacac atatcgttca tctgccggat gcgattgcgc gtgatattag
     4501 cgtgcaactg catacagtca tcgctcagta tcccggcgcg gagctggagg cgaaagggat
     4561 ggcttttgcg ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg agaggtacca gtaaaggtga ggcaattgca gcttttatgc aggaagctcc
     4741 ctttatcggg cgaacgcccg tatttctggg cgatgattta accgatgaat ctggcttcgc
     4801 agtcgttaac cgactgggcg gaatgtcagt aaaaattggc acaggtgcaa ctcaggcatc
     4861 atggcgactg gcgggtgtgc cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagac gagcacgccg ccagtgccgg tggccttgcc gttggcatac
     5041 tgggggcact gaaagccgca ggcggactgt ggtttggctg gagtggtgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaaag gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcccgctt
     5221 ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa
     5521 acgatcgtct ggcgttcctg gattgtcttt ctaacctgac ccgcgtcacg acacgtagcg
     5581 caaaaagcca tacagcctgg ggcaaagcat ttcgaacaga agtctacccg atcggcattg
     5641 aaccgaaaga aatagccaaa caggctgccg ggccactgcc gccaaaactg gcgcaactta
     5701 aagcggaact gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag
     5761 gtttgccaga gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg
     5821 gtaaaattcg ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagctg gacgaattaa tggtaaatac gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtagcaa
     6061 aagagtatgt tgctgctcag gacccagcca atccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa cccctacgat cgtgacgaag
     6181 ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta
     6361 cctttccaaa gcttgcgtag gagcggttaa tctcccgtaa gtggaactaa cagcacatcg
     6421 acctcacttg aggcaataac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatgcttgt gacatacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg cgtttcttcc tgcatcacgc tgcgtaaatc ttcaagcatc
     6661 ggcgcagcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta
     6781 accgcaacag cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac tcggtcaaac
     6901 aggtaaatat tagtctgatt attagtaaag ac
//
LOCUS       DAPHJE010000180         6930 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPHJE010000180 DAPHJE010000000
VERSION     DAPHJE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.182"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2757)
                     /locus_tag="RH222_004142"
     CDS             complement(<1..2757)
                     /locus_tag="RH222_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV3799068.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNT"
     gene            complement(2814..5186)
                     /locus_tag="RH222_004143"
     CDS             complement(2814..5186)
                     /locus_tag="RH222_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV3799069.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5224..6909)
                     /locus_tag="RH222_004144"
     CDS             complement(5224..6909)
                     /locus_tag="RH222_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV3799070.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1789 a         1594 c         1562 g         1985 t
ORIGIN      
        1 agtatttacc ggatgagaaa gatattgttt aacggcagtg ttaacattct ctaccgtcat
       61 ttgtttcaac aattgctcct gctcagtcca tgctgcagga tcgtcatatt gaataagact
      121 atttacaata gtgttcgcta attgttgaac gctacgctgt tggatatcga ggctgcgctg
      181 aacgttttgc tggtattcat tcagttcttg ctcactgatc cctttagcca gacgcttaac
      241 catcacttca ttcgctaacg ttaacagttc atcatgtcgt tctggttgac aagtaaaagc
      301 cagcaaatga ctgatatctt tggcctgagg atcaaccgag aggcgagaag aaacgctgta
      361 tgctccagat gcctgttcac gaatattaac acgtagatct tttgccagtg cgacgttaaa
      421 agcatcgagc gccatacgcg tcgccagatt aacaggtgtc cgggaatcat aacgcttcca
      481 ctgtgaaacc tgtgccacag gttcattttg ttcttttaca gtaaccgatg cgttgtccgt
      541 cgcgcgagtt aatggtttac ctgcggctaa tggcgaatca gagtgtttga ttgatcctaa
      601 gtaacgcgta attaacgcca cgagtttgtc ttctgagaca ttaccgacaa tgacaaacgt
      661 gatatccgct ggagatgaaa acaactggcg atcggcagcc agcgcatctg cggcagtaaa
      721 ctgtacaatc tgattttctt gcagtaattt cgtgcggtca tcagcatagc gcgtctcata
      781 catctgctgg gcgaatttct ccgctggacg ctggtcgagc gttttcaacg cctgaatttg
      841 agcattttgt agcgatgccc aaatattgtc gttaatcgtg ctgtgggtga ttcgctggtt
      901 aatcaactga aaaccaggtt cagggttatt agtccgcgcg ctaacggaga gcaacgtatt
      961 catgccactg actttactgc tcatggttac cgaattttcc gcactccagc gtttcaggct
     1021 ggaagaggag agctcaccaa cgccgcttcc gcttactgct ttatttgcca gcgcgataag
     1081 tgatttttgc tgcgcaggga aacttaaatc gcctttattc gatacggcag taatttgtag
     1141 cttttgctct tcacccgcgg attttgccag aataaccctg gcaccattgg aaagtgttaa
     1201 tgatgtcaga ttctcagcca gcgtttcttt gctgctaatt tccgcctgtg ggtcagcgtc
     1261 tactgttaac gataaatttc tgcctgggaa gatgtaagcc gccagctttt tgttggcgta
     1321 ctctttttcc agcgccagga tagccgcagg agacaatgct tttttggcgg caagctcatt
     1381 gtttaccatt tgctcccaaa atgcgtcctg gttctttctt aactgctgcc atttttccgc
     1441 cagactttgc acggtaattt gctgccacag acgtttcgaa agttgatatg tctcttccgg
     1501 cgacaagaac ggcgtattat ttaatgagct ggatgccagg cgactggtca gcatacgtaa
     1561 atcacgttca gcttgctgat caaccgcatt tttcagccag gtgaggcggg tagatttgac
     1621 atcatcgagt tcttcagcag aaaaaccatg ctgatcaatg gttgccaact ctgccattaa
     1681 tgcattcgca gcatcctgca tattatcgtc tcgtgcattt acacggaaaa acagcgactg
     1741 ataatcgggt gcaattttaa cgctgcgcgc agtgccgcca gaaatagtct tcaactcgcc
     1801 cgactgtatg cgttcctgca gacgttgatt gaacagctga actaacatgc tccattcagc
     1861 ttgttcgaca aagctttgct catcgttcac ttgtaccatt ggcaggcgat aatagagtgc
     1921 gatgccgttc acccggtttt ctttatcatt aatgatatta aagcgcaggt ggttttcggc
     1981 ttttgtcggc cagacgcgat tttcagctgc tttgttagcc ggaagcttac ttaaattatc
     2041 ctttatcagc gccagcgctt ctttactgtc gatatcgccg accacgataa aggtcatatt
     2101 atttggttga taccagcgtt gataaaattg gcgcaattgt gccggtgtga ccgtggcgac
     2161 agtatccatc aggccgatag gttcacggtc taaattacgg gtatttgcca gcaggaaagg
     2221 gcggcgcgcc tgagaggtgc gccatttcgc atcctgatgg gcacgccatt cctcagtaat
     2281 tacgccacgt tcagcgtcta cttcgagttt ttcaaaggtt gcggcattac tccattcact
     2341 gaagattgcc atcacttgtt gcagattttg tttctgcgta gtcggcaaac tcacctgata
     2401 caccgtttcg tcatagctgg tataggcatt aacatcgcga ccaaaacgca ggcccattga
     2461 ctcaaatgtt tcgatgactt tattacccgg ccatgttttt gtgccgttaa acatcatatg
     2521 ttctacaaaa tgagccacgc cgcgctcatt gtcttcttcc tgcaatgaac cggtatgaat
     2581 ttgcagccat aaatttacct gatcctttgg atgagcatgc ggataaatca tatatcgcaa
     2641 gccattgtcc agttgcccgg taattaactt ttcatcctgc ggtaaggcgg cggcaatcag
     2701 ccgcccgggg agcaacagag ttgccactaa cgtcagtaag aaacagaggt ttctcataat
     2761 tatctccatg cgaaaaccgg gcgaatttac ccggttaagt aaaatccgaa ctattaaaat
     2821 ttcatgctga catcaagcca gaaagtacgg ccactggcgt aggtcgccac accggtgttc
     2881 gttgtatcaa cagcggtttt gctatccagt acattgagaa tatcggcact gatcgtcagg
     2941 ttttgttgtt tcagaaattg tggcgtccag gacaagcggg tgtcccaggt caggctgcta
     3001 tccagttttt cgtcaacata ctgcttgtaa tctgaatatt cgctgatgta ttgcgcattt
     3061 gtcttaccaa ggataatgcg agctttacgc gcttcttgcc aggccagcgt attcgcccac
     3121 actaacccgc tcggttgatg cgtgaaatcc atgtttaagg agatctttaa tgggttatta
     3181 aaatctgcca ctggaacgct atcgtaagag accagattac cgttataaac cacctggtta
     3241 tcaccggtat tgctctcctc ataaccattg tttaacgaca agttgccctt gctcttgata
     3301 tagctaaaga caatttgtgg gttaatatct acctggcgga tatgcagggg ttcggccagt
     3361 tcaaaactga ggctgaacga atgcgttttg gttttgccat cgttgttata ttcagtaatg
     3421 gtggttttag tcgcgctgtc ggtacgactg cttttgctga tttgatcatg cgcttcacgg
     3481 taaacatagt ttgcacgcgc aataacgttc ttaccgattt tctgctgcaa tcccattgcc
     3541 agttcatcgt tataaggcgt tttcaaatcc tgataacgcg tcagagtttt attacctgat
     3601 accgattccg tccagctatt gcggatatca cgtaatccca tatcaagaat attcccgcca
     3661 tagtaacggt tataacctgc tgtaatcatt gaggtttgat tagcaaaaat atcccattcc
     3721 gtcataaagc gcggggagat attgtggttt gacagatagt tgtcatagtc ataccgcacg
     3781 ccgggcatta atgacacatt acgccagcta atgcgatccg ccatatacag cgtgtagttg
     3841 tcaattccca ggcggccttt acctttatgg taaatggtat ggttagtttt ctttccggca
     3901 gcattaatca cataggattc agactggtta tggcgttcag tccacgcatc ggaatagatg
     3961 tattccgcgc cgaagtaggg ttgatgcgaa acattaccca cggcgaattt ttgccagtcc
     4021 aggcgtgttt tgaaggtgta attatctaca gcctgggaaa tgtgtcctaa tccgccacgg
     4081 gtgcaacgcc ctgtaatatc accatatgta catgaaagtt cggtgtacca gatgtcatga
     4141 tcgtgacggg tataatcact aatatgatcc caaccaacgg tggtacgcag tttggcccat
     4201 gcgagctgcg tatccatatc ccatgccaga ccataggatt tattacccat ttcgcgatca
     4261 gactgcggga aggtgctggt attataatca cggctggagc cggtatattt taaggttaaa
     4321 tcgtgggtaa agcggtcgct ggcaaaccag gtaaatttgc tcaatgcagt atcgataacg
     4381 tttttatact gtgcccgacc ggcgacaatg ccgtcattcg aaacataatc cgcgcgggtg
     4441 atatcagact ggcggcgcga taaaccggcg gtaacgccga agttatcagc gagttcctga
     4501 ttaaacgaca aggtataaaa gttcttttta aaatctgggg agtaataagt acttcctgaa
     4561 gagccttgat taaatgcgct cttgttattc tcatcgatat gcgatgttaa ccagtccgaa
     4621 cgcgtagtgc gataacccaa tttcaccttg ctatcatcag cgttgaagcg tttgatcttt
     4681 gcatcaatta ccccgccatt gaagcgacca aattcaaccg gcacaaaact gtcataaagc
     4741 gtcacattgt ccagtaagct gacatcaaga taatacccct gtgacatccc gctaatattg
     4801 gttgcactac tggcatcgga ctcattcgct gggttcaggt tattagttgc actaataccg
     4861 tcaatcaaat aggcattctg gtagggcgac gcaccgtgaa tagagatttt ctcagggcga
     4921 atatctccct ggttcaacga ggtactttgc gttgaatcca tgcgtaccgc agggttggtt
     4981 ctcagcagat cgctgatatt gccattcccg gtcggtaaac gctcgatgct ttcactggtg
     5041 taatgcgtgt tgccgtttac gggtacaggg acaggagcat agaccgtcat ctcttcaaaa
     5101 aaatgcatgt acatgttttt gtcatcgacg gcctgcgcca catcagcgcc acataaaatg
     5161 acgccaggaa taagaactcg cttcatttat ttatatctcc gtacagcagg gcttttattg
     5221 ttgttataaa accgtgctaa tgtcacaaat atcatcagcc aggttccaga caccgggttg
     5281 atgtgtaacc ataatgacgc cgcttgtggg tagtttttca cgcactaaac gcagtaagcg
     5341 tatagcctct tgttcctcaa gatgagaggt agtttcgtca agaaatatcc atttcggacg
     5401 tcgtaaaatt aatcgtgcca gggcgatacg ttgtttttcg ccgctggaaa gaatatctcc
     5461 ccagcgatcg tggtcatgaa tacgcgcagc caatttcccc agaccaacct gatgcagtac
     5521 ttcgctcaac gatttatcgt ctacgggcag gggaagtgct ttacaaataa tctctttcag
     5581 taagccgctt ttgattaacg gtgtttgtga cacataccaa ctgtcagcag gagaagaaat
     5641 atcaccttta aaccacggcc agcagtggga taatgtttta agcagtgtgg tttttcccgc
     5701 accagagtag cctttcagta atagccattt gcctggcgaa acatgaaagt tcaagttctc
     5761 taatatgatc ttattatcag gcgtacgaat actcgcatta gccacttgta ccgcatgttg
     5821 gcaattttta ggcttattcg tagggcgctg ttcagtgagt tgatggaact catacaagcg
     5881 atcgataacc gcagccagct cagcaagttc gtcatattta taaataaacc agcttaaatt
     5941 gttcgatact agcataaatg cctggcgcga tttcattagt ccgcccagat tgatctgccc
     6001 gctaataaac tgcggcaata acagaaagta gggaagaacg ctaagcgagc gcgaataaat
     6061 attttgccag taatcaagcc accgctgacg attcattaaa cgatgccagt tctctttaat
     6121 ggtatgaaaa ttgtcgcgca actcctggcg ttgtagagac tcagcattgc ttagcgcaat
     6181 cagttctgcc tgcttattat gctgcacaag attagttcga aatgtcgctt cgctacgttg
     6241 tttttccaca ttaagcggac gaatacgttt acccacctta tgggtaaata aagttccacc
     6301 gatcacaatg agcacaacgg tatagaccat atacccttgg atattccatt ctgttccacc
     6361 aacagtgaat gagagcgtac ccgcgctttg ccaaagaata acggtaaagg tgatcagcat
     6421 gctaagtgac tggatgaagc caaatgaaag gctgagcgtt ttgctgatca gtaagagaat
     6481 gtcttcagcg atacgttggt cagggttatc tgtattttta tgctcaccgt agatctgcgt
     6541 gaagtagtag tttttatctg caaaccaccg attaaggtag taatctgtta accattcgcg
     6601 ccagcgaata gtcagcagtt taattaacca tgttttgttt acggatatta atacaaagat
     6661 ccccagcagg gcagggaacc aaaggacaag ctgccaaagc ttatcggttt ctttctggct
     6721 tagcgcattg aaaaaatcat tattccagtc gtttaaccag acctgaattt taaccacgcc
     6781 aaggatcatg gcgaggataa taatgattaa caacactgaa gttttattat tcttacgcag
     6841 ccaaaagggc ttcagcagac agagatactt tgctatcaac atacaaaacg taatgggaat
     6901 ggttatcatt agcgaaaatt gattgtgcca
//
LOCUS       DAPHJE010000181         6912 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPHJE010000181 DAPHJE010000000
VERSION     DAPHJE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.183"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(126..1775)
                     /gene="pgi"
                     /locus_tag="RH222_004145"
     CDS             complement(126..1775)
                     /gene="pgi"
                     /locus_tag="RH222_004145"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV3799071.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     gene            2300..3649
                     /gene="lysC"
                     /locus_tag="RH222_004146"
     CDS             2300..3649
                     /gene="lysC"
                     /locus_tag="RH222_004146"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV3799072.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAAVQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3794..4720
                     /locus_tag="RH222_004147"
     CDS             3794..4720
                     /locus_tag="RH222_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDV3799073.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4756..5547)
                     /locus_tag="RH222_004148"
     CDS             complement(4756..5547)
                     /locus_tag="RH222_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDV3799074.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5553..6680)
                     /locus_tag="RH222_004149"
     CDS             complement(5553..6680)
                     /locus_tag="RH222_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDV3799075.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1713 a         1787 c         1646 g         1766 t
ORIGIN      
        1 atcacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt taaatcacag
       61 acaaggggat ttatctgata aaacaatgcc gacataacgt cggcattgtt attaaggttt
      121 ttaaattaat cgcgccacgc tttatagcgg ttaatcagac cattggtcga gctatcgtgg
      181 ctgctgattt ctttatcatc tttcagctct ggcagaatac ggtttgccag ttgtttaccc
      241 agctccacgc cccactggtc gaaggtgaag atgttcagga tcacgccctg agtaaagatt
      301 ttgtgctcat acagcgcaat caacgccccc aggctgaacg gagtgatttc acgcagcagg
      361 atggagttgg tcgggcggtt accttcgaac actttgaacg gcaccacgta gtcaagcgtt
      421 gccggatctt taccctgatc gcgatattcc tgctcaacca cttcgcggga tttaccaaac
      481 gccagcgctt cagtctgagc aaagaagtta gacagcagtt tctggtggtg atcagagagc
      541 gggttatggg tgatagccgg agcgatgaaa tcgcacggta ccattttggt tccctggtgg
      601 atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt caccccagat aatcgggcca
      661 gtctggtaat ccacaacgtt accgttacgg tcaacatact taccgttgga ctccatattg
      721 ccctgctgga agtacgccgc gaaacggtgc atatactggt catacggcag aatcgcctca
      781 gtttccgcac caaagaaatt gttgtaccag atgccaatca gcgccagcag tacaggcagg
      841 tttttctcgg caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc agaaagcagt
      901 tcaacgaagt tatcaaagcc gatggagaga acaatcgaca ggccaatcgc tgaccacaaa
      961 gagtaacggc cgccgaccca gtcccagaac tcgaacatgt tggcagtatc aataccaaac
     1021 tcgccaacgg ctttggcatt ggttgagagc gccgcaaagt gtttcgcaac gtgtttttca
     1081 tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat ggtttcctga
     1141 gtggtgaagg ttttagatgc taccaggaac agcgtggttt ccgggtttac ttttttcagc
     1201 acttccgcga tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag gtggtttttg
     1261 tacggacgca gtgcttcggt caccatgtac gggccgaggt cagaaccgcc gatcccgatg
     1321 ttcactacgt cagtgattgc tttgccggta taccccttcc actcaccgga gataatcgct
     1381 tccgagaagg ttttcatctt ctccagcacc gcgttgactt ccggcattac gtctttgcca
     1441 tcaaccagaa tcggggtatt gctacggtta cgcagcgcta cgtgcagcac ggcgcggttt
     1501 tcagtgcggt tgatcttctc gccagagaac atcgacttaa tcgcgcccgc cagatcgcac
     1561 tctttcgcca gatcctgtaa tttcgccagc gtctcttcag tgatgcggtt tttggagtaa
     1621 tccaccagca tctgatcgtc gaatgttgcg gagaacttag aaaaacgatc gccgtcttta
     1681 gcaaaaagat cggcgatcgt aacgtctttc atttcatcga agtgtttctg tagtgcctgc
     1741 caggcagcgg tctgcgttgg attgatgttt ttcattagca atactcttct gattttgaga
     1801 attgtgactt tggaagattg tagcgccagt cacagaaaaa tgtgatggtt ttagtgccgt
     1861 tagcgtaatg ttgagtgtaa acccttagcg cagtgaagca tttattagct gaactactga
     1921 ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc ccgctcaccc
     1981 tttatttata aatgtactac ctgcgctagc gcaggccaga agaggcgcgt tgcccaagta
     2041 acggtgttgg aggagccagt cctgtgataa cacctgaggg ggtgcatcgc cgaggtgatt
     2101 gaacggctgg ccacgttcat catcggctac aggggctgaa tcccctgggt tttcaccaga
     2161 agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa atagtagcga
     2221 agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct gaaaatggat
     2281 cccctgacac gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg gtaccagcgt
     2341 agctgatttt gacgccatga accgcagcgc tgatattgtg ctttctgatg ccaacgtgcg
     2401 tttagttgtc ctctcggctt ctgctggtat cactaatctg ctggtcgctt tagctgaagg
     2461 actggaacct ggcgagcgat tcgaaaaact cgacgctatc cgcaatatcc agtttgccat
     2521 tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc tactagagaa
     2581 cattactgtt ctggcagaag cggcggcgct ggcaacgtct ccggcgctga cagatgaact
     2641 ggtcagccac ggtgagctga tgtcgaccct gctgtttgtc gaaatcctgc gcgaacgcga
     2701 tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt accaacgacc gatttggtcg
     2761 tgcagagcca gatgtagccg cgctggcgga actggccgcg gtgcagctgc tcccacgcct
     2821 caatgaaggc ttagtgatca cccagggatt tatcggtagc gaaaataaag gtcgtacaac
     2881 aacgcttggc cgtggaggca gcgattatac ggcagccttg ctggcggagg ctttacacgc
     2941 atctcgtgtt gatatctgga ccgacgtccc gggcatctac accaccgatc cacgcgtggt
     3001 ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga tggcaacttt
     3061 tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca gtacgcagcg atatcccggt
     3121 ctttgtcggc tccagcaaag acccacgcgc aggtggtacg ctggtgtgca ataaaactga
     3181 aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc tcactttgca
     3241 cagcctgaat atgctgcatt ctcgcggttt cctcgcggaa gttttcggca tcctcgcgcg
     3301 gcataatatt tcggtagact taatcaccac gtcagaagtg agcgtggcat taacccttga
     3361 taccaccggt tcaacctcca ctggcgatac gttgctgacg caatctctgc tgatggagct
     3421 ttccgcactg tgccgggtgg aggtggaaga aggtctggcg ctggtcgcgt tgattggcaa
     3481 tgacctgtca aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg aaccgttcaa
     3541 cattcgcatg atttgttacg gcgcatccag ccataacctg tgcttcctgg tgcccggcga
     3601 agatgccgag caggtggtgc agaagctgca ttttaatttg tttgagtaat atttccctac
     3661 tggcgtataa cgctcaccga ttgttatacg ccatcctcac tgaaaacata gattaaataa
     3721 tatctgaaaa gcaaaatcac tcctgtagaa cagtattata atcaccgaaa aatatcattt
     3781 aacaggagtg ttaatggcta ctttaatgga aaaagatgca cttttaaacg gagccagtca
     3841 gtgcatcgcg tttttatcaa atatcattga taattgctca gtttcgtcgc atcaggattc
     3901 aggcgatgct ctcaagcgat tagtcagcta tcgcgattat ctttattcta cccccgctga
     3961 acttgttgat tttacacaag gtaagattct ccttcagcag gtaagaactc aatatcagca
     4021 tgaattcaac aatactacgc attcagaaaa taaagcatct tttgattcta tttggcagcg
     4081 tctgacaaat catgaagtca caccgcaaca gcatcccatt ggttttgttt taggtgggca
     4141 acccggagca ggtaaatcat ctctgattga attagccaaa agagagacaa aagataatat
     4201 aatgattatt aacggtgatg attttcgatt tctacaccct gattttaatc atatctacca
     4261 aaattatggg gatgattttg ttacccatac agcgaaattt tccggcgaaa cggtcgaacg
     4321 tgcaattgag agagcgatag tcagtaaact caacattgtt gtcgaaggaa catttcgtaa
     4381 tgccgctacg ccgttacaaa cattaaaaaa actcaaagat gcaggctacc aaacagaagt
     4441 catgataaaa acaacctcag cggcattaag ctgggaaagc accaatgaac ggtataataa
     4501 ggacaaagag gcgggaaata ttgcccgaaa agtggataaa aaccaccatg atatcgtcac
     4561 cggtttgctg gcagaaaatg cccgcaaggt gtttgcttca aacctggcag ataagtttgc
     4621 ggtgtattcg cgtgagaaga tgatatttag ttcacaagcg gcaacgaatg acgatattgc
     4681 aacgcttata cagaatgaaa tcagcgggaa tacacaataa taataagagc tgatggcgtg
     4741 aaataacgcc gggctttatg cccggcgcat taccgtctgg tacattaagc tatccagttc
     4801 cttaacgtcc ccttctctgc tatccggtaa cagcggtgga atttccccac gcgccagttc
     4861 cgcacgaata aacgcatgta acaatggacg gcgcggacca tattccgctt ctcctgcgcg
     4921 ctgtttacgt tccagtaatt cgtctatttc tgcacgtaac ggcgcatcaa gttcgcttcc
     4981 cgccagtaac tcagcaaagc gcattggcgg tacgccttta cccgcttcga cccagcgcac
     5041 ggccagcagc ggacgcagca cgtaaaagta ttttttcagt cgaacttcgt cgccctgcaa
     5101 atatccgggg aagtttttct gcgccatcga atagtaatgc cagcgcgcac gcagcggaga
     5161 aaaccacatt gggaccattg ccttaaacgc cgttatcgtt tcttcatctt gttgataaac
     5221 caccggagaa tccagccatt ctatcagcgt cgggttcgcg cctttcaata atcccagcgc
     5281 cttacgccac tcccaaccgg aaacatctaa ctcgtcgtcg atgggcagct caatgacatc
     5341 gcgcggtgac tctactcgca gataccattc cagcgggtga acatacaaaa accgtacatc
     5401 gtagtcgctg tcgggcgagg caaatcccca gccgcggctc cctgattcac aggcgtacag
     5461 tactttcaca tcataccgcc gttcaatttc ttttaactgc tgcgataccc tttctcgcat
     5521 cgcagcactg actccattta atggcatgtt actcaccctt tgatattaac caacccatgc
     5581 agaacctgat ccagaccgcc aaatacagag atgttatcga tccgttcagc cactctctcc
     5641 agcgtttcca gttcttttaa gcgtaacgct accgggttgt tttccatgac tttagcggta
     5701 ttgagtaacg aacgcgtcgc tgccgtttct tcacgtcggc gaatcacatt ggcctgcgcg
     5761 gatttctccg cctcaaccag ctgcgcaagg atatttttca tatcgccagg caggacgata
     5821 tctttcaccc ctaacgaggc aatctccata ccaaatggca acatgcgcga tttcacctgt
     5881 tcgctgacaa cgtcatcaat gacctgttta tcttccagca attcatccaa cgtacgcgta
     5941 cctaccgctt cacgcagggc aaattgtaat tcccgataca aatgatcaat cggcttcgtt
     6001 aactggctaa atgccaataa cacgtcgctg tagcgccagt tagcggcgag gttaatccgc
     6061 agattgacct tatctttcgt taagatttct tgcccgctga cttccagtac ctgcaagcgg
     6121 gtgtcaacca cttcagcgtc aaccagatgg ttaattttcc agtatgcggt cagccccggc
     6181 ggtaacagcg cctgcgtttc accatcgatt ttcagcacgc ctacatgcca ggccggaacc
     6241 tgcaccgtta gtattgcatc gcggccttta actgcccctt tgcgccgtgg ttgcaatacc
     6301 gcattcatca cctccgtttg tacctggact tgctgcgtat tcatccgcac cagtgtcaaa
     6361 tcatcctcta cacgccagta caaacggcgc gttgatggcg gcaggatctc ctgcaaaata
     6421 ccgtccatat acaacgcccc ggcttcaatc tccgacaagt ctgccaccag gcaatatttc
     6481 tccacccaat caggctgaaa gcggcgcaaa taatccgcca gcacgtcggg aacttcgctg
     6541 ccatcgagcg taatcagcaa gacttctggc gtatttaacc agggtaaaag atgctcgcct
     6601 gcatgtagca ctttgtagta atcgccgttt ctggagagta atgccagctg atcttttctt
     6661 acgcttattt ttttaatcat tatctattcc ttgcttaaca gccgtcgcgg aagcagacac
     6721 gaagcgacag gggaattccc ttttgcctgt tacccggcat ctttatcggc gacgttatcg
     6781 gggatcgctg actgcgcaat ccctcacttt ttatttaata agttaaaacc agtattcatt
     6841 tcaggaatga atgtgggaat cgaacccata attggcgtca aaggctgacg gaacacgccc
     6901 ggaaaacagc ct
//
LOCUS       DAPHJE010000182         6881 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPHJE010000182 DAPHJE010000000
VERSION     DAPHJE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.184"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..382)
                     /locus_tag="RH222_004150"
     CDS             complement(<1..382)
                     /locus_tag="RH222_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3799076.1"
                     /translation="MNGKRYPTQKEINELYEYNSETGLFIYKRRESVRECWNSTYAGK
                     IAGSIDEKGYVRISVNKKVCRAHRIAWISFYGSEPDGEIDHINGIKSDNRICNLRVVD
                     DKQNSRNRKKPINNRSGVIGVAYYK"
     gene            complement(379..669)
                     /locus_tag="RH222_004151"
     CDS             complement(379..669)
                     /locus_tag="RH222_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDV3799077.1"
                     /translation="MVNLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGAGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKP"
     gene            complement(669..1391)
                     /locus_tag="RH222_004152"
     CDS             complement(669..1391)
                     /locus_tag="RH222_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDV3799078.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEKKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(1384..1560)
                     /locus_tag="RH222_004153"
     CDS             complement(1384..1560)
                     /locus_tag="RH222_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDV3799079.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCEHCCVELM
                     SDPNSSMYEEEDDE"
     gene            complement(1553..1900)
                     /locus_tag="RH222_004154"
     CDS             complement(1553..1900)
                     /locus_tag="RH222_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 domain-containing protein"
                     /protein_id="HDV3799080.1"
                     /translation="MDYSQLSDFEINKRVFKAIVGAKPLGYPHNADGRSVGNESNGNY
                     RWYDYCNNPEDAEPIIVENRIGIIPAPENGLWKAAHRKVGSDSTPYHMTQDENPLRAA
                     MIVFLMMQDANNA"
     gene            complement(1903..2079)
                     /locus_tag="RH222_004155"
     CDS             complement(1903..2079)
                     /locus_tag="RH222_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDV3799081.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(2076..2603)
                     /locus_tag="RH222_004156"
     CDS             complement(2076..2603)
                     /locus_tag="RH222_004156"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDV3799082.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            complement(2600..3040)
                     /locus_tag="RH222_004157"
     CDS             complement(2600..3040)
                     /locus_tag="RH222_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDV3799083.1"
                     /translation="MKKLTFELRSPIHQQNAIQAIQKILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVNEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(3021..3203)
                     /locus_tag="RH222_004158"
     CDS             complement(3021..3203)
                     /locus_tag="RH222_004158"
                     /inference="COORDINATES: protein motif:HMM:NF025712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HDV3799084.1"
                     /translation="MSELKPCPFCGDNAMIMRIPESGWRYGQCMSCFSNGSKRPDYEG
                     AVIAWNRRAGDEETNV"
     gene            complement(3206..3526)
                     /locus_tag="RH222_004159"
     CDS             complement(3206..3526)
                     /locus_tag="RH222_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799085.1"
                     /translation="MDVSRKQFQSWFADEIVGADVEFPEFEDGEYVAGEIYDEQLYVM
                     LQAMYMAWTASRAAIEIELPAKNDISGDDHPIPDLVDWDDGRNAGIQECAEAIRAAGI
                     KVKE"
     gene            complement(3704..3910)
                     /locus_tag="RH222_004160"
     CDS             complement(3704..3910)
                     /locus_tag="RH222_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799086.1"
                     /translation="MAEFMLVAFKCVGVGWILLAFFIVLHSYISLVNGGKDPFYMLFG
                     AVFTWVLIGIVPVAIAKMAWCFIN"
     gene            complement(3987..5867)
                     /locus_tag="RH222_004161"
     CDS             complement(3987..5867)
                     /locus_tag="RH222_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV3799087.1"
                     /translation="MSPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFADGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            complement(5864..6355)
                     /locus_tag="RH222_004162"
     CDS             complement(5864..6355)
                     /locus_tag="RH222_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 motif-containing HNH endonuclease"
                     /protein_id="HDV3799088.1"
                     /translation="MSLEVGEYIPGYEGKYSVTKDGRVYSHLRGRFLNPSPDNKGYLR
                     VTLCSDGIQKTLKVHRLVAQLFIPNPYCKPEVNHINGDKSDNAIWNLEWCTCSENLKH
                     AFSIGLKSAKGVFNGRAKLSETDVIDIRNSVGMKLSELSTKYGVSEMQISTIRRGNAW
                     RHL"
     gene            complement(6466..>6881)
                     /locus_tag="RH222_004163"
     CDS             complement(6466..>6881)
                     /locus_tag="RH222_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV3799089.1"
                     /translation="ETITKEKRNNKNTMSESVRTECEKSPDRHEETDKAFEEIFWCAG
                     MRKAGKKNAASAFRTQFREWRKTTRGTASEFATMLAEDIACRNGKQFGFDRLLPSSYL
                     NGQRWNDEKPETIQPQSKPSSAITVSKTGYVFFDR"
BASE COUNT         1593 a         1828 c         1417 g         2043 t
ORIGIN      
        1 tcttatagta ggcaacacca attactccag agcgattatt aataggcttt tttctgttcc
       61 ttgagttctg tttatcatct acaacgcgca aattgcatat tctgttatcg cttttaattc
      121 catttatgtg gtcaatttcc ccatcaggtt cacttccata aaaggatatc catgcgattc
      181 tatgagctcg acaaactttt ttatttacag atatacgaac gtatcctttt tcatctatag
      241 aaccagcaat cttaccggca tatgttgaat tccagcactc tcttacactt tctcttctct
      301 tgtatataaa caaacctgtt tcgctattat attcatacaa ttcattgatt tctttttgtg
      361 ttggatatct tttaccgttc atggcttaat ctttccctcc ttcagcagta tcgcctgcgt
      421 cctgatcacg ccttcgaggt ggtaaagtct ggcgtctttg ttgtcgaggt tatgggtgcg
      481 tcggtcgatt tcatcgtgac acgcgctaca agcccatgca ccgatcaggt cgtcaggctt
      541 cattcccgct ccgcaaattc cagccatccg gtaatgtgcc agaactgtag tttcaggatt
      601 accattgcat acgccgtaaa tacgtacctg gcattctctg ccgcgcgctt ctttgcgtag
      661 gttaaccatt aagcagcctc tcctgttact ttcagcattc cgttatcgag cagctttctg
      721 gtcagccact gttggccacg tccggtgatt tttgtggtga acgatatctg tattccgtga
      781 tttgtgttga ccgctgtttc tttcactgtg aaatagccgc gatccatata ttcctgcatt
      841 ggcacattgc gccgggaacc tgaagcaata aggattttgt gatcgcgcat ccacgcaaac
      901 agtttgtttg gaccaattcc aacaaccttt gcaaagtttc caatcaaaat tccgctggcc
      961 tcgccaacgc gatcggcaaa ctcaacttta ggtgcggcaa ttgcgagctg gttttccagt
     1021 tgcattttct tctcggcaag atcagcagca aggcgcaacg cttctggtag cgttttgggg
     1081 atattaaccg cagcttcttc aagctctcgc cagcggtcaa caaggcgagc ggtgaactct
     1141 ggcgacaact gggcaacaac aacaatacta tctcgcttac cttgttcgcc ttcgaagacg
     1201 taatgctcgt actgaacatt gaaccctaag ttattgattc tttcggaaac ctcaatttga
     1261 ggaagccgga taacaccatt tttagccagc gtttcgatgg tacgtttcac attgtcatga
     1321 cgcttaccca ccaactcagc gatttcaatg cttgtcattt ttatggcatt gccatttatt
     1381 aactcattca tcgtcttctt cctcgtacat tgagctattc ggatcgctca tcagttctac
     1441 gcagcagtgc tcacacacgt gaacttccag cacatgcagc ttctgaccgc agttagcgca
     1501 cgttaaagcc cgctcgacgc tttctttctg gtattgaagg gattgggatg ggctaagcat
     1561 tattggcgtc ctgcatcatg agaaagacaa tcatggcagc gcggagtggg ttttcatctt
     1621 gagtcatatg atatggggta ctatcactgc caacttttct atgcgctgcc ttccataatc
     1681 cattttctgg cgctggaata atgccaattc tgttctctac gataatcggc tctgcatctt
     1741 caggattatt gcagtagtcg taccatcgat aattaccatt tgattcattg ccgacagacc
     1801 gtccatctgc gttgtgcgga taacctaatg gttttgcccc tactatcgct ttaaatactc
     1861 gcttgttaat ttcaaaatca cttaactgtg aataatccat tgtcatttcc tcgcacgatg
     1921 tcttagccac cggatatccc acaggtgagc cgtgtagttg aaggttttta cgtcagattc
     1981 ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt ggaaggtatt tgcagttttc
     2041 gcagattatg tcggtgatgc ttcgtcgctg tcgcctcatg ccgccctcct gacgccctgc
     2101 ccgatcgcca tcaatgccgc tttggatacg gtagtaaaca ttcgtcgagg actgatgaac
     2161 ggtcgccaaa tcagcagcat ggagcctttg ctgtttccct tcttctccag ccctgtcgat
     2221 ggttcgataa aattaatccg cccatcagtg ataatgcgaa cttcgtcgac actctccaga
     2281 gccttgctga accatccgac tgacatatcc tctggcacaa gcataactac cgtctgtcgc
     2341 tgttgtatgc actgctcagc ggctttttcc acccacggcc tgatattgct gtacggtggg
     2401 ttattccaga ttgcaccgtg gcttatccac tcagaattta gcgcgtcgtc ggcctcagtt
     2461 aaccagtgag cgcacagagc atttttgtcg ctcgctgccg aatccagcca gaatccaaac
     2521 tcaatatcca gtgcatcaaa aagccaaagc ggcgtttgcc agcagtcctt gtcgtgtgct
     2581 ggcgtatttg atttgatagt catgcagccc gatctcccca tcgagctttc cattcgagag
     2641 ccagtcgcgc ttcgtctgac cacttaacgc cacgctctgt accgaatgcc tgtataagct
     2701 ctaatagctc cgcaaattcg tttacacgca tcctgctggt tgactggcct attaccacaa
     2761 agccattccc ggcaaggtta ggaacaacat cctgctgctt taatgctgcg gtaaacacac
     2821 acttccagct ttctgcatcc agccagcgac catgccattc aacctgacgc gagacgtcac
     2881 caaggcaagc ccaaagcttc ctgttttggt ctaagctgcg gttgcgttcc tgaatggtta
     2941 ctacgattgg tttggttggg tctggaagga ttttctggat ggcttgaatg gcgttctgct
     3001 gatggatggg gcttcttagt tcaaacgtta gtttcttcat ctcctgctct ccggttccat
     3061 gctattaccg cgccttcgta atcaggcctc tttgagccat tgctaaaaca tgacatgcac
     3121 tggccatatc gccatccact ttcaggaatg cgcataatca tcgcgttgtc tccgcaaaac
     3181 gggcatggct ttaactcgct catactcact ccttcacttt gattccagcg gcgcggatgg
     3241 cttctgcgca ttcctgaata ccagcgtttc ttccatcatc ccaatcaacc aggtcaggaa
     3301 tggggtggtc atcgccggaa atatcatttt ttgctggcaa ctcaatctcg atagctgcgc
     3361 gagatgcggt ccaagccatg tacatagcct gaagcattac atacaactgc tcatcataga
     3421 tttccccggc aacatattct ccatcttcaa attcaggaaa ctctacatct gcgccaacaa
     3481 tttcatcagc aaaccacgac tgaaactgct ttcttgatac gtccatattc ctctccatca
     3541 ctcgcctaac actttcattg cattagcgat ttcttcaatc tggtcatctg cctgattaaa
     3601 tattcccata ctgagtcatc ttcttccact tcgtaatacg ctttaagcgc gtcaaaaatc
     3661 actgaatagt taatctcgga tattttcatc attactctca cttttagttg ataaaacacc
     3721 acgccatttt tgctatcgct acaggtacaa ttccgataag tacccaggta aacacagcac
     3781 caaacaacat atagaatggg tctttaccgc cattaacaag gctaatgtag ctatgcagaa
     3841 caataaaaaa cgccagaaga atccatccaa cgccaacgca tttgaatgcg acgagcataa
     3901 actcagccac gatttactct cccacaaata aaaaggcctg cgattaccag caggcctgtt
     3961 attagctcag tgatgtatat ggtcatttaa tactccgtca cgttttcctg tcgccacgcc
     4021 tcgtcatatt ccgatttcgg catattggcg atgtagctat atggcgatcc tgattcaagt
     4081 tgcaggaact ggtgcgattg ctcgtcaagg aacaacggga caccaccttc ccaaccttcg
     4141 ccgttacgtt gtttttcaag catcaaaaca gatgccggag atgccagtag ctgttcgtcc
     4201 ttctctgaca tcttttcacc actctgaact ctctgtaacg ctctctcgcg agccttgtta
     4261 cgccagatga tgaaaaggtt gtctgtcagg tctgttatcg ctccagagcc ttttacgtcc
     4321 attttcccgg ttggtttttc ttcgctgtct ccttttcgcg agtgagtaac gagaatgacg
     4381 tgggagtttg ttttgttttt gaagtcgcaa atcgagtcaa caaacgcctt ctgcccgtta
     4441 tagtcatcgt cgcctatgcc acatttcatc aggctgtcga tgatgaataa ctggatcccg
     4501 tatcggcggc gagcgtagtc gaatatttcg atcagcctgt cggctttcgc cgttccggtc
     4561 aggccaaaca cccagagtct ttcgtcataa aatttaaatg cagagtcaat ttccagcact
     4621 ggcggcatct tgcagcacgt cgcctgacgg gtaagtcgct taaggagaat gcctggcttc
     4681 agctcaagtg acgcgatgca cgtcttcaca ccctgacgca ttgcctcaag tgccatatgc
     4741 ccgacaacct ccgtttttcc gtgaccgttc acaccattga ccagcgtcaa ctcggcctca
     4801 cggaactgga atttatctgc cagagattcc cacggtggat taaacagata ctgctgcttg
     4861 ccgtagaaag cgttgatagt gtcctggtaa aactctcgcg cgctgtagag ttcttcagga
     4921 tcgaagtagg atgccgtgcc gatgtactgc cagatttcat cctcggtaac accgttcatc
     4981 aggcattcgt tgatgtcttt gtacggcaga gtaacaagac ggcaacgatg ttcaccgagt
     5041 cggcttgcga tttcccttgc ggcttcacga ccaacatcat caacgtccat cgagatgaat
     5101 atttcctcaa acctgtcgag gttgtgatac tcaaactcaa tccactgttg cttagcgcct
     5161 ttcccgccac caaacggcac ggataacgcc gagatgccgt attgcgcata gctcatacaa
     5221 tcaatttcgc cttcgcaaag tacaaccgcc ctcacgccag cgtccagagc ctgccatccg
     5281 aacagacaag gttcgcaatc accttctgcc ataatgactt tcttcccgtc cgggcgctca
     5341 gtgctgattc gcttgacctg caacaactca ccatcgcgtt tgtacggaag caccagtgca
     5401 tcaagttctc gttctccatt ccacaccttg ccgctgacaa cctcgtagcg ctttacgact
     5461 tctggcgata tgccacgcga ttgcaggtac tcaagatggg attctgttct ggtaacgtag
     5521 cgggcgattt tcttgcggtc aggtctggag aatttcttct cacgtttggc atcgaaatgg
     5581 tgatcgtcat ccttgattcc gagaaaggct ttcgcttcct gcatagcctg atgcaggtta
     5641 attccacgac atgccatcca caaatcaagc atgtcaccgc cgtctccatc agcgaaatca
     5701 gcccattttt tcttgccgct aaggttgacc ttaaggctgt ttcccttgtc accgttgacg
     5761 ttaccggcaa cccactcatg cccctctttc ttgccgtttg gcaacaggtg cggagccacc
     5821 ctgtcaacct gcgcccaaag caggtcgcta agttctgatg gactcaaagg tgcctccatg
     5881 cgtttcctct gcggattgtg gaaatttgca tttcactcac tccgtattta gtggatagtt
     5941 cgcttagttt cataccaaca gaattcctga tatcaatcac atcagtttca gatagttttg
     6001 ccctaccatt aaaaactccc tttgcgcttt tcagtccgat gctaaaagca tgttttaaat
     6061 tctctgagca ggtacaccat tccaagttcc aaatggcgtt atccgatttg tcgccgttga
     6121 tatgatttac ttctggcttg caatacggat ttgggataaa taattgagct acaagacgat
     6181 gaacttttaa cgtcttctga atcccatcgg aacacaaagt gactcttaga taacccttgt
     6241 tatccggaga tggattcaaa aacctgcctc ttaaatgtga ataaacacga ccgtctttgg
     6301 ttacagagta tttaccctca taaccaggta tatattctcc aacttcaagg ctcatcatga
     6361 ttccctcaga ttgagatttt taaaccagaa atcgacaaac gaaatactta accagccgtg
     6421 gttataacca gcgaccagta gcgatttgat ttttgatttc atggttcacc tgtcgaaaaa
     6481 cacgtagcca gttttcgata cggtgattgc ggatgatggt ttggattgtg gttgaatggt
     6541 ttctggcttt tcgtcgttcc agcgctgacc gttcaggtag ctcgatggta acaacctgtc
     6601 gaatccgaac tgcttaccat tcctgcatgc gatatcttct gccagcatcg tggcaaactc
     6661 gcttgccgta cccctggtag ttttacgcca ttccctgaac tgtgttctga atgccgaagc
     6721 cgcgtttttc ttcccggctt tccgcatgcc tgcacaccag aatatttcct cgaatgcctt
     6781 gtcggtttct tcgtgacggt caggtgattt ttcgcactcc gtccgaacac tttcggacat
     6841 agtgttttta ttatttcttt tttcttttgt aatagtttct t
//
LOCUS       DAPHJE010000183         6879 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPHJE010000183 DAPHJE010000000
VERSION     DAPHJE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.185"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(266..751)
                     /gene="sixA"
                     /locus_tag="RH222_004164"
     CDS             complement(266..751)
                     /gene="sixA"
                     /locus_tag="RH222_004164"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV3799090.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(954..3098)
                     /gene="fadJ"
                     /locus_tag="RH222_004165"
     CDS             complement(954..3098)
                     /gene="fadJ"
                     /locus_tag="RH222_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV3799091.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3098..4408)
                     /gene="fadI"
                     /locus_tag="RH222_004166"
     CDS             complement(3098..4408)
                     /gene="fadI"
                     /locus_tag="RH222_004166"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV3799092.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4588..4872)
                     /locus_tag="RH222_004167"
     CDS             complement(4588..4872)
                     /locus_tag="RH222_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV3799093.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5244..6584
                     /gene="fadL"
                     /locus_tag="RH222_004168"
     CDS             5244..6584
                     /gene="fadL"
                     /locus_tag="RH222_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV3799094.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1726 a         1847 c         1621 g         1685 t
ORIGIN      
        1 tttcaattac atcctgcctg attattcaac tctattatct gtattaataa atgacaatga
       61 gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa gatacgcgca
      121 gaagaataat ttccgaatga atgtagaaag tgttttattt atagataagg agagtcttcg
      181 atagtagtga ggttgacaga tatccgctgc ctgatgagca attatgccca atgaatctac
      241 ctcattgggc ataatttgga acacatcaga tagcttttgc catcttcaga ttacacgggc
      301 tcatctgcca gttgaatgta ccgtttccgc tttcatcaag cgttacgctg gcaatggccg
      361 aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga tacccgacta
      421 acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac gcctgcaaat
      481 aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact tctgcactgg
      541 aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc aggaacgggc
      601 tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc atcaggcgag
      661 attcgtcaca accattagtg gtcagaggac gaaccgaatc actggcggca tcgagggctg
      721 cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa ccagattcgc
      781 ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt gccttatcca
      841 ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa tatgcgttta
      901 cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc ttcttattgc
      961 aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc gaccaaacgc
     1021 tcgcaagggg taaaacggga accatactgc gtggcaagtc gttgcattat tgcaaccacc
     1081 tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg cggaaaacca
     1141 atgccaaata ccgcaccaat atccccgtca cgcacgctac ggataacctg ctcatcaaca
     1201 caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg cggtgcggag
     1261 agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg cgggatcgac ctgttttttg
     1321 cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct gcctttgcga
     1381 tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc tccataagcg
     1441 gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc caaaagttgg
     1501 attggaccta ccggaaaacc aaatttcact agcgcagcat caatgtgctc tacccgttca
     1561 ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg attgacgtaa
     1621 aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc cagttttacc
     1681 gtagtggcga tggtttgcgc tgatgtaccc gcatgaggaa taatctccac cagcggcatt
     1741 ttttccaccg gactgaagaa atgcaggccg ataacttgct caggtcgcgt ggcgtgagcg
     1801 gcgatatcac caatcggcaa agatgacgta ttcgaagcaa agatggtatg agcggcgcaa
     1861 ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa caccgcttca
     1921 ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat taatgccagc
     1981 tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc cagctgatcc
     2041 caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat tctgaccgga
     2101 agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc accacctaaa
     2161 attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt cttcacttcc
     2221 gtactggcaa aaaagatact acgcaaagct tgcgattgtg gcgtcatcgc cagttcgcca
     2281 aacgcccgag cttcggcgtc ataaccgctg ctggtgccct gcgctaatcc cgtttcaaca
     2341 acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc tgttttctta
     2401 ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg ctcgcgtaca
     2461 ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcgac ttccagcaaa
     2521 atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt cgcccgaagt
     2581 tgttttccgg tgaggatcat ctctaatgct gtgctgacgc ctatcagacg cggtaaacgc
     2641 tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc gagcaccgtt
     2701 ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccaactccag cccaccaccc
     2761 aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat ctccgccatc
     2821 aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca gttgccgatc
     2881 atgttgatgt ctgcgccagc aatgaagttg tccggtttag cggagacaaa caccacgcct
     2941 cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga ggcaaactcc
     3001 gccttcaggg tattcatttt ctcacccggt acgtcgatgg tgatgatagc aatgttgtcc
     3061 agacgaacat taagagtaaa cactgatgcc atttccatta ttccgcctcc agaaccattg
     3121 ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg ccgcgacggc
     3181 gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag ggatgcccgt
     3241 aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca gtggcatgtg
     3301 cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata ttcgccagtg
     3361 tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag atcgctcatt gtcaaaccgg
     3421 cacgttccag cgccagcggt gttgaccagg ccggaccgag caacatgtcc tgccagacat
     3481 caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct aattctttcg
     3541 cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc gtactgtttg
     3601 ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg taatcggcaa
     3661 gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa ggagggataa
     3721 aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg gcacgctgat
     3781 gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt ttcgccattt
     3841 gctctgcggt gtcacccatc cgcaagccgg tagaatattc tgctactgca ggcggtacgg
     3901 gcattaaatc gcgcaaacgc aggcgagaga agagtttcag gcgctggctc atggttcgag
     3961 ctttgttgac atcaaccagc acgcgcgcca gttttttact gacgccaatt ggcaatacgg
     4021 aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc aggctttctg
     4081 cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg taagcatcgg
     4141 tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg gcttcaggca
     4201 tttgtacgac ctgaccaaag accagttgtt caatcacttc ggcggggatc tcgctgcgtg
     4261 ccagcaattc gcctaccacc atcttcccta aatcaaccgc gggaatgcca tgaaaagccg
     4321 tcgcctgacg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga tcgccctggc
     4381 gggtaaccag cggtaaaacc tgacccataa cactcccctg taaaaaaata aataaagtgg
     4441 tctgacctga tcatagtctt aaccattttt ttacatttag ccaagcggag aaaaaggaaa
     4501 gtgggagcta tgacacagtg aagacaggag aaagaaaaga aaacgcccct gccagcaact
     4561 gacaggggcg aacgtacccg agaggaatta acgcagaccc agctggaaga tcagcatttc
     4621 agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact cttcagtgaa
     4681 agtcggggag attttgcacg gttcggattc cacgctacgg gctttttcag tcagcgccgc
     4741 cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg cggtgcagtc
     4801 ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt catcagcact
     4861 acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat aaacgttgtg
     4921 gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa tcggataagt
     4981 gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg cgagtgcgca
     5041 acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa aagatggaaa
     5101 catacttgca acattccagc tggtccgacc tatactctcg ccactggtct gatttctaag
     5161 atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag tttgtataaa
     5221 aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag tctgctctcg
     5281 cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag ttaaacgaat
     5341 tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc gatgatgcag
     5401 gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca ttttctgcgg
     5461 gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct ggtcgtagcc
     5521 tgaaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt gttgcaccga
     5581 ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg gcaacagagt
     5641 ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa accatgaacc
     5701 tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt ggtttcaacg
     5761 ccgtctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag ttggttgctg
     5821 gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg gcagctaccg
     5881 ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag tggggctttg
     5941 gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca ctgacctacc
     6001 gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat cgtgcgttta
     6061 ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg ggttatctga
     6121 cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat ccacagtggg
     6181 cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg aaagcgacct
     6241 caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct taccgcatcg
     6301 cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt atcgcctttg
     6361 atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag gaccgtttct
     6421 ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac gttggtgttt
     6481 cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc gagtctgaag
     6541 gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc gttcgcctgg
     6601 ataaagtcac ctgcatagca ggtgacttta actcccccac ttcaccgaaa gtagtgtcct
     6661 cattgcttac ctcctgagtt ttgcaaacag cctgttggca gcttgccctt tcaaatcaat
     6721 aagcggtatc cacagacaaa cctgaaggga aaggcatatt ttcaggcgtt ctgctcgtcc
     6781 ttctcaaaga gtttactttt ctgcatttcc aggatactca acccccctgg ctattgtgcg
     6841 ctcatacact caaattaaag ataggttcta aataaatga
//
LOCUS       DAPHJE010000184         6821 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPHJE010000184 DAPHJE010000000
VERSION     DAPHJE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.186"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..163
                     /locus_tag="RH222_004169"
     CDS             <1..163
                     /locus_tag="RH222_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799095.1"
                     /translation="TITLVILFCRRPHCRKVSVRQYYQKRVIFYPTTFQRTAFKKCLF
                     LMRDIIISS"
     gene            124..243
                     /locus_tag="RH222_004170"
     CDS             124..243
                     /locus_tag="RH222_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV3799096.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(722..988)
                     /gene="minE"
                     /locus_tag="RH222_004171"
     CDS             complement(722..988)
                     /gene="minE"
                     /locus_tag="RH222_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV3799097.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(992..1804)
                     /gene="minD"
                     /locus_tag="RH222_004172"
     CDS             complement(992..1804)
                     /gene="minD"
                     /locus_tag="RH222_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV3799098.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1828..2523)
                     /gene="minC"
                     /locus_tag="RH222_004173"
     CDS             complement(1828..2523)
                     /gene="minC"
                     /locus_tag="RH222_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV3799099.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3043..3411
                     /locus_tag="RH222_004174"
     CDS             3043..3411
                     /locus_tag="RH222_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDV3799100.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3531..3932)
                     /locus_tag="RH222_004175"
     CDS             complement(3531..3932)
                     /locus_tag="RH222_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV3799101.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4174..4467
                     /locus_tag="RH222_004176"
     CDS             4174..4467
                     /locus_tag="RH222_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV3799102.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4539..5198
                     /locus_tag="RH222_004177"
     CDS             4539..5198
                     /locus_tag="RH222_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV3799103.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIIPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5290..5736
                     /locus_tag="RH222_004178"
     CDS             5290..5736
                     /locus_tag="RH222_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV3799104.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRDNLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5943..>6821)
                     /gene="hlyE"
                     /locus_tag="RH222_004179"
     CDS             complement(5943..>6821)
                     /gene="hlyE"
                     /locus_tag="RH222_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDV3799105.1"
                     /translation="VVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQEYS
                     QAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASAQK
                     DILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQVD
                     KIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTLSNTV
                     KQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTCNE
                     YQKRHGKKTLFEVPEV"
BASE COUNT         1752 a         1523 c         1592 g         1954 t
ORIGIN      
        1 aacgattaca ttagtcattt tattttgtcg acggcctcat tgtcgaaaag tcagcgtacg
       61 acagtattat cagaaaagag tgatttttta tccaactaca tttcagcgca ctgcgtttaa
      121 aaaatgcctc tttcttatgc gggatatcat catttcatca tgatgtcttt ggtgagcggt
      181 gaacacaata cacctgcgct gtctcttcag gatgaatccc ctggtctggt gcctgcgggc
      241 tgatgttgca gccgagctaa ggtcacttag acgctactat catttatccc atggcatgga
      301 gtcaaaatca gtcgataccc gcagtatata tcgtgaactg gtcgttctga ccatcaacga
      361 ttaaactgcg cttcggcaca gttttcgttt acaggatgtt aaaagagaaa attctggggc
      421 aaaaaaagcc cgccagttac ggcgggaaac ctcatcctat gggagaacaa tgaataatga
      481 aattgcgggg ttatcatctc ccagtgtatc cacactaaca ataaggttat ttactcagcc
      541 aggcataaac attttgtttt gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga
      601 aatagcaatg aggagtatca gcaagaatac tcgccgcttt accacaacgt ggatgagagg
      661 gatgaaaaac tcaaggcaga gataactctg ccttgaagat aaatgcgctt ttacagcggg
      721 cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc
      781 catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa
      841 tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac
      901 ggcgttcagc aacaataatc tgcagccgtt cttttgcaat gttggctgtg tttttcttcc
      961 gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc
     1021 ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg
     1081 tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt
     1141 tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga
     1201 tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc
     1261 gcgttaacag caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgattttg
     1321 acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg
     1381 taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg
     1441 gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga
     1501 ccccttcacg ggtgagggca tctttatcgc gtgtttgcga tgccggcaga atatagagat
     1561 tttcagtacg cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt
     1621 tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga ttacgcaggc
     1681 cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg
     1741 ccgcgctgga ggttgtctta ccaacacccc ctttgcccga agtaacaaca ataatgcgtg
     1801 ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc
     1861 gactaactgc agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca
     1921 gtattcacct gcgatggaca ccagttccgc catcaggttc gtacaaaata tttgcgtttc
     1981 ccggtcacca cttgcccctg ccagcgcacg accgcgcatc atgccataga catgaatgtt
     2041 accatcggca attaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg
     2101 tggagcataa atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac
     2161 cggcgttgta ttttgcgctg gagcctgcgg tgcgggagct ggacgtggcg ctttttcctt
     2221 tccttccgtc aggataggca gccccatttt ttcaatttcg gctttaagtt gcgcatcttt
     2281 gcagccactt acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga
     2341 ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa
     2401 tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc
     2461 atgcagatga accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga
     2521 catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga
     2581 ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga
     2641 gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa
     2701 tcaacccatt gccgtcgtac ttttgattct tcttatttac gcttcctttt tccgcaccct
     2761 aatcgtgaca gcatctccag acctgtttaa atatccagta ttgaggcaag tgtatttttt
     2821 caccacgcca ggctttctta actttccatt ttttgcggca tcattaaaga acagataccc
     2881 gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata
     2941 cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgatga
     3001 aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt
     3061 ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc tggaaatgac
     3121 gctggcggac ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat ttgtgtgaac
     3181 tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac
     3241 ttacaatcat tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc
     3301 gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtagatgg taagcggagt
     3361 ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg atgccttgtt
     3421 tacattatgt tctattcggc attgtctgct attcgtcatt aacctcttga gcgacagaga
     3481 ggagtggggc gttaacgttt atgacgggga gagtccccgt cggttgacat ttacttaatt
     3541 tgaatatcga cgttatattt tttagttttg tttttacggg cgtcgtttcg ggtttgcagc
     3601 actcgcagct catatttgcc agaagcaggg agtgagtatt gtccgtgact atcgagttct
     3661 ggggaatatc tggataaatc aacagaatca tcgattcctg gcccgaagag gtaagtatca
     3721 gcgccttcat tagagatact cacatgcact ttctgacctt ttttggcata gaaggtatat
     3781 gtatcgtaat cgtatccctt tatttcgccg gagtactgag cgctgctatg gccttttctg
     3841 aactcaacat tgacattctt acccgcagca aagctcgtgg aggttagcag tgcaagcagc
     3901 aatacagcct tactgatgct cttgattttc attggtagtc tcctgttcag aatgagtacg
     3961 atgaactgtt ataatataac aatcccttac gggagaattc cagtggcttt cctgcgttcg
     4021 gtaaaatccc gtcgcggcat ttttgccggg gggatgactt ataaaggggg catggactca
     4081 acaaaccgta gaaatctaca gctggctggc ttgtggcgcg ggtttcattg atgttagctt
     4141 atgccaaaac ctggcatctt aaagagtaaa agtatgtttt gtgtgattta tcgaagcagc
     4201 aagcgtgacc agacctattt atatgtcgaa aaaaaagacg atttttcgcg tgttcctgag
     4261 gaactgatga aaggttttgg tcagcctcag ttagcgatga ttctgccgct ggatgggcgg
     4321 aagaaactgg tcaatgccga tattgaaaag gttaaacagg cattaaccga gcaaggttac
     4381 tatttgcagt taccgccacc acccgaagat ttgctgaagc aacatctttc cgtcatgggg
     4441 cagaaaacag acgacactaa caaataaccg atatccggcg gtggcattat ctttgtcggc
     4501 gcgggttttc atatccacga taaggtgagg ggaacgttat gtatcaacat cacaactggc
     4561 aaggtgcgct gctggattat ccggtgagta aagtagtctg tgttggcagt aactatgcca
     4621 aacatattaa agagatgggc agcgcagtgc ccgaagagcc agtgctgttt attaaaccag
     4681 aaacggcact gtgcgatctg cggcagccat tggcgatccc atccgatttc ggttcagttc
     4741 atcatgaagt cgaactggcg gtgttgattg gcgcgacgct gcgtcaggct acggaagagc
     4801 atgtccgcaa agccattgcc ggttacggcg tggcgcttga tctgacattg cgtgatgttc
     4861 agggaaaaat gaagaaagcc gggcagccgt gggaaaaggc taaagcgttt gataactctt
     4921 gtccgctttc cgggtttatt cccgcggcgg aatttaccgg cgatccgcaa aatacaacgc
     4981 tgggcctgag cgtaaacggc gaacaacgcc agcaaggtac gactgcggac atgatccata
     5041 aaatcattcc gctgatcgct tatatgagca agttttttac cctcaaggcc ggtgacgttg
     5101 tgttgacagg cacgcctgat ggcgtcggcc cgttgcaaag cggtgatgag ctgacagtca
     5161 ctttcgatgg gcattctttg acaactcgcg ttttgtaata ctttttgccg cctgaaagcg
     5221 gcggcaaaac ttgcatcgct gtgccagact ggttataagg tgcgttttaa cgtaatggcg
     5281 gaacacctga tgagcgatgt acctttctgg caaagtaaaa ccctggacga aatgagcgat
     5341 gcggaatggg agtcgttgtg tgatggttgc ggtcagtgtt gcctgcataa actgatggat
     5401 gaagacaccg acgaaatcta cttcactaac gtcgcctgtc gccagctcaa tattaaaacc
     5461 tgtcaatgtc ggaactacga acgtcgtttt gagtttgaac ccgactgcat taaattaacc
     5521 cgtgataatc tgccaacatt cgaatggctg ccaatgacct gcgcttatcg tttgctggca
     5581 gaaggtaaag atttacctgc gtggcatccg ctacttactg gttcgaaagc ggcaatgcat
     5641 ggtgaacgta tctctgtgcg tcatatcgca gtgaaagaat cagaagtgat tgactggcag
     5701 gatcatatct taaataaacc tgactgggcg cagtgataaa tttaatagat gctgatgggg
     5761 gctttcttaa tatacgtctg gatgctcgcc atttcctagc cctgtttttg aaagcgctct
     5821 gtcataatga gagttacccg atacattatt atcaacaggt aactctcaat ctttaaaaaa
     5881 cgtctgtatt tttaaatgtg ataaacttta taccttgagt acatggagag aataatcgct
     5941 gatcagactt caggtacctc aaagagtgtc ttcttaccgt gtcttttctg atactcatta
     6001 caggtgttaa tcattttttt ggccgcttct tttagcaaag aaagcattaa atcatcataa
     6061 tcaacataga atctggttgt ttcagtttcc gtttttatct ccccgatggc ggctatttcg
     6121 gtggttaatt tcaatttggc ggcatcgata tctttattcg cttgtttaac cgtgttagac
     6181 agggtggtaa agaaactctg cacagatttt aacttgttct tcaattctgg aatcagtttc
     6241 ccttcaacta cgcccgcagc aatagaatag gaaatgatta aaccaaatgg accggcgacg
     6301 acaccggctg cggcaccggc atacgcttcc ttcctgattt tatctacctg tgactggaaa
     6361 tagctgcttt tttctgaaaa atcattggtt aactggctat ctaacgccag cagtttcccg
     6421 gaagcgttgt tgaaactttg tgagcttacc agcagggatt tttgcgcttc attcagcttc
     6481 gtgatgccgt catccagtac cttaatgaga atgtctttct gggcggatgc tttcttctca
     6541 ttgtactcat caaatagcaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat
     6601 tcatacactg tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta
     6661 atatcgccga ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct
     6721 tttatggttt catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga
     6781 tctaatgctc catctgcggt ttcgattgcg tttttaacta c
//
LOCUS       DAPHJE010000185         6806 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPHJE010000185 DAPHJE010000000
VERSION     DAPHJE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.187"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..109)
                     /locus_tag="RH222_004180"
     CDS             complement(<1..109)
                     /locus_tag="RH222_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV3799106.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNI"
     gene            322..1113
                     /locus_tag="RH222_004181"
     CDS             322..1113
                     /locus_tag="RH222_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDV3799107.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1123..1965
                     /locus_tag="RH222_004182"
     CDS             1123..1965
                     /locus_tag="RH222_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV3799108.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            1975..2751
                     /locus_tag="RH222_004183"
     CDS             1975..2751
                     /locus_tag="RH222_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDV3799109.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2761..4302
                     /locus_tag="RH222_004184"
     CDS             2761..4302
                     /locus_tag="RH222_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV3799110.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4299..6380
                     /locus_tag="RH222_004185"
     CDS             4299..6380
                     /locus_tag="RH222_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDV3799111.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6401..>6806
                     /locus_tag="RH222_004186"
     CDS             6401..>6806
                     /locus_tag="RH222_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV3799112.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSI"
BASE COUNT         1642 a         1684 c         1818 g         1662 t
ORIGIN      
        1 caatattatc gaccctggcc ccttcataac catattcggc aaattcatta accgcgcata
       61 acagcagctt ttccttcagg ctctgtacgt tgctggtatc aagatacata acgcttcccc
      121 acgacctctt caagataact atcttaaccc gattaattag aagctccagt aaaaaggata
      181 aaaacttgta ctttcatcaa gttatcaaat gtactgtgat cactatcgca tatcaaccaa
      241 ccagatggtt gatctttctt gcggcacgtc gcaagctaat tgtcacttag aaaacaatat
      301 tcaaaaaaag agagctattg tatgaaatcc actcgtcctg ttgccgtgat taccggagcc
      361 gctcgtggta ttggaaaggg ctgtgcgctg gagcttgctc gtggcggatt taacctgctg
      421 attaacgatc ttcccgatgc cgacagcgta gaaaaactgc atatcacaca acaagaatgt
      481 atagctgaag gcgtggaagt gatttgcttc cctgccgatg tcggcgatct ctccttgcat
      541 gaagagatgc tcgacgcggc gcaaaaccag tggggacgtc tggactgcct gctcaacaac
      601 gcaggcattt cagtgaaaaa acgcggtgac cttctcgacc tcgaaccaga cagcttcgac
      661 caaaatattg ccattaacac ccgcgcgccg ttcttcctcg ctcaggcatt cagtaaacgt
      721 ctgctcgcac agccgaaacc ggaagcagaa ctgccgcacc gctcgattat tttcgtcagt
      781 tccatcaacg ccatcatgct ggcgatgaat cgcggcgaat ataccattgc caaaaccgcc
      841 gtttctgccg ctgctcgtct gtttgctgct cgcctttgta atgaacaaat tggcgtttat
      901 gaagtgcgcc ccggtctcat caaaaccgat atgactattc cggcaacggc ttattacgac
      961 gaactgattg ccaaaggtct ggtgccgtgg ggacgttggg gttatccggc tgatattgct
     1021 tccactgtcc gcgcgatggc ggaaggtaaa ctgatttaca cctgtggtca ggcggtcgcc
     1081 atcgacggcg gcttaagtat gccgcgcttc tgaggctgaa tcatgagaga tctgttacaa
     1141 cgcccggatt tattcagtat caataccgca acactgggct ataaaacgcc gctacccgcc
     1201 attattgatg cctgcgccgc gcgtggtatt ggcgcgattg ctccctggcg cagggagttg
     1261 caaggtgaag atctccagca aatcgcccac cagcttgccg ccagtaacat gagcgtttcc
     1321 ggtttgtgcc gtagcactta ctacaccgcg ccgacactgg cggaacgcaa actggcgatt
     1381 gatgataacc gccgggcgct ggacgacgca gcggtgctta acgccgcctg ttatatgcag
     1441 gtggttggcg gtctgccaac gggcacaaaa gatctgtacg aagcgcggga acaagtaaaa
     1501 caagggattc gccagttgct gccacattcg aaagatgtcg gcgtgccaat tgcgctggag
     1561 ccactgcacc cgatgacagc tgccgatcgc tcttgcctgt gcacgttacg ccaggcgctg
     1621 gactggtgtg atgaactcga tcccgatggt gagtttggcc tcggcgtggc ggtggatgtt
     1681 tatcacgtct ggtgggatcc ggatctcgcc agtcagatcc tgcgtgccgg aaaacgcatc
     1741 ctcgcgtttc atgtttccga ctggttagtg ccgaccactg atctggtcaa tgaccgtggt
     1801 atgccgggag atggcgtcat caatattccg tcaattcgtc ggctggttga aaacgccgga
     1861 tttaatggtg ctattgaact ggaaatattc tcgccttact ggtggcagaa agatattaac
     1921 agcacgctgg atattagcgt cgatcgcatc gcgcattatt gttaaggaat gatcatgagt
     1981 gaccaacccg ttctgttcag cagagtggcg gcttcctgcc gcctgacatt gaaccgtgaa
     2041 gataaatgtc atgctattaa cgaagagatg atcgaatctc tcgaccatta tttaaatgaa
     2101 attgaaaacg acaccacatt acgattagtc gaattaacgg caaccggcga taaattcttt
     2161 tgtgcgggcg gcgatattaa atcgtggtcg gcctattcgc cattagatat gggcagaaaa
     2221 tggattaaac gcggtaatga cgtttttaat cgcctgcgaa acttacccca attaaccgtt
     2281 gcgaatctca acggtcatac catcggcggt ggtattgaac tggccttgtg ctgcgatatt
     2341 cgtattgccc gccccggcgc gaaattctct aacccggaag tgatgctcgg catggtgcct
     2401 ggctggatgg gcattgaacg tgtgcttaat caggttggtc cggtggtcgg tcgccagatg
     2461 ctgatgctgg gtaaacgcct gacggcgcag gaagcccagg cggcgaattt aattgatgaa
     2521 gtggtagaaa tagaacaggt ggaaagctgg atggcgaacc agttagcgca gctggaaaaa
     2581 tgcggtccgg tggcactggc gcatattaag caactgattc tggcactgga aaataaacat
     2641 gccgattacc cgcaccagct actggccgga ttaatgtccg ccactcagga ttgccagcag
     2701 gcgacgcgcg cctttgccga aaaaagtagc gtgagcttcc ataatcaata aggaaattgt
     2761 atgcgtaaga taacaaccgc cgaggcactg gcggcgcaaa ttcaggacgg cgcaaccatt
     2821 gccattagcg gtaacggcgg cggcatggtg gaagccgacc atattctggc tgctattgaa
     2881 gcgcgtttcc tgcaaaccgg acaccctcgc gacctgacgc taatccactc gctgggtatt
     2941 ggcgatcgcg actgcaaagg cactaaccgt tttgcgcacg cagagatgct caaacgcatt
     3001 attgccgggc actttacctg gtcgcccaaa atgcaggcgc tggtaaaaaa taacacgatt
     3061 gaagcctact gctttcctgg tggcgtgatt caggcactgc tacgggagat cggcgccgga
     3121 cgtccggggc tttttaccca cgttgggctg ggatcgtttg ttgatccacg caatggcggc
     3181 ggtaagtcga atgaatgcac taccgacgac ctggtagaac tgattgaaat cgacggtgaa
     3241 accaaacttc gttatcgccc tttcaaagtg gattacgcga ttttgcgtgg cacttatgcc
     3301 gatcctcgag gcaacgtcag ccttgaagaa gaagcgattg atatggatag ctattccatg
     3361 gcgctggcag cacacaacag cggcggcaaa gtgttcgtac aggtacgcga tgtgctggaa
     3421 gctggcgcga ttgaaccacg ccgggtcaaa ttaccgggca ttctggttga tggcatcgtt
     3481 gaacaccgcg aacaaccaca aacctatctt ggcggttacg acctgaccat cagcggccaa
     3541 catcgccgtc tgagttctaa cgacgccatt gaactggtta gtcatccggt acgtcgcctg
     3601 attgcccgtc gggcggcacg cgaactggta gctggcgctt caaccaactt tggctttggt
     3661 attcctgggg gtattccagg cgtagcgctg cgcgaaggcg tgccttacca aagtttatgg
     3721 ctgagcgtcg agcaaggcgt gcataacggt atgatgctgg atgatgcgtt tttcggctgc
     3781 gcccgcaacg ccgatgccat tattccatca ctcgatcaat ttgagttcta cagtggcgga
     3841 gggatcgata tcaccttcct cggcatggga gagatggatc agtacggtaa cgtcaacgta
     3901 tcccatctca atggcaatct cattggtccc ggcggttttc tcgaaattgc gcaaaacgcc
     3961 cgtaaagtgg tgttctgcgg cacgttcgac gccaaaggta gcaagattga tgtaacgcca
     4021 gatggcttgc atatcaccca gtcaggtcaa atccctaaac tggttaccca ggtggaaaaa
     4081 atcactttta gcgccgccta cgcacagcaa agtggtcagg aagtgttgta tatcactgaa
     4141 cgcgcagtat tccagttaac ggcagaaggc gttgaattaa ttgaaatcgc gccgggtgtg
     4201 gagattgagc gcgacattct gccgtatatg gctttccgtc caattatcaa gcacccacgc
     4261 ctgatggaaa gtagcctgtt tacgccgatg gaggatgcat gagtcagctt gatgacacca
     4321 tccttgatgc gctgacgcac gttactttcc caaagggttt tgcacaggca gagcccgcat
     4381 gggttgtcac ggtagacggt gttgattacc cactctggca aacagatgcc ctggtagtcg
     4441 gcagtggtgc agcaggatta cgtgcggcag ttgaacttaa acgccgccag caaaatgtgc
     4501 tgatcgctac cgccgggtta tatatgggga cgtcggcatg ttctggttcc gataaacaga
     4561 cactatttac cgccgctacc gcgggcaacg gcgacaactt caccaaactg gcagaggcac
     4621 tggcgagcgg tggggcgatg gatcacgaca ccgcttatgt cgaagcggta ggttctctgc
     4681 acactctagg cgggttgcaa tatcttggtc tggaattacc ggaagatcgc tatggcgcga
     4741 ttcttcgtta tcaaaccgac catgacgaag ccgggcgtgc aacctcgtgt gggccgcgta
     4801 cctcaaggtt aatggtgaaa gtgctgttgg aagaagtaca gcgcctcgcc attccagtgt
     4861 tgaccagtgc aacagtgatt aaactgctgc atcagcgtga cgaaaacggc gaagaccgcg
     4921 tggcaggggc aatcctcgcg accggtcatc gcgcccataa cccatggggg ctggcaattg
     4981 tgactgcgcc caatgtggta ctggcaacag gagggcctgg cgagctttat cgcgacagtg
     5041 tttatccaca taaatgcttc ggctcgctgg ggctggcgct ggaggaaggt ctgacgctaa
     5101 ccaatctgac cgaaagccag tttggtatag gcacgccgcg tagcacgttt ccgtggaatt
     5161 tatccggcac ctatgtacag gtgatcccgt atatctattc cgtggatgct gagggtaacg
     5221 agtataactt cctcgcggat tactatcgca ccacccagga gctggcttca aacattttcc
     5281 gtaaaggcta tcagtggccg ttccacgcca cgcgggtgat ggattttggc tccagcttgt
     5341 tagatatggc agtagcgcaa gagcagcaat cagggcgtca ggtatttatg gatttcaatc
     5401 gcaatcctga agcggtgccg ggtgacctgc cgttctcatt agatcgactg gacgacgacg
     5461 ttcgcgcgta tctggaaaat aacgacgctc tggcaccatc gcccatcgaa cggctgcaac
     5521 gaatgaatcc gctgtctatc tcgctgtata agatgcacgg ttacgatctc accacacagc
     5581 ctttgcagtt tgccatgaat aatcagcata tgaatggcgg cattgaagtg gatatctggg
     5641 gacaaacctc cctgcccggc tgtttcgccg tgggggaagt tgctggcaca cacggcgtca
     5701 ctcgccctgg tggtgcagca ttgaatgccg ggcaggtttt tgctgttcgt ctggcacgtt
     5761 ttattggttg cacgcaaaag cggaatattg atggagatat agcacagctg gtagctccta
     5821 cactggcttc tataagagag atcattactc aggcgcacga taacgggacc gggatgccgt
     5881 tgtcggttgt gagagaaaaa attcaggcac gaatgtctga ccatgcggga tttatttgcc
     5941 atgccgataa agtccgacgc gccactcgcg atgccctgct attgagcgaa tttgtccaac
     6001 ggcatggatt ggctatcaaa catgtgggtg aagttgccga gctgtttatg tggcggcata
     6061 tggcactgac ttctgccgcc gtattaacgc aattaacgca ttatattgac gcaggtggtg
     6121 gtagtcgggg agcacgtatt attctcgaca gggatggaaa ttctattccg caaacacgaa
     6181 atggtttttg tgatgcctgg cgctttcgtt cagagcgtac tgaggataaa aaagataaac
     6241 tacttattca ttattgcaac ggtatctttc acgtcagaga aacacctgtt cgtgagtttc
     6301 ctattatccg aggtatttgg tttgaaaaaa actggcctgg ttttcttaat gggacaattt
     6361 accagccaca ggatgaatga tattgcaaag gttaatcact atgcgaaata taattagatc
     6421 tattaatgct ctgcttgcag cattgaacat taccattttg gcgatcatcg tggcatgtgt
     6481 gacgtggcag gtggctgcac gatttatatt tactagcccc agtattttta cagatgaact
     6541 cagccgtttg ctctttattt gtcttggact ttttggtggg gcatacaccg cagggcaaaa
     6601 ccggcatctg gcgattgacc tgctacctat gatgctgaaa ggaaaagccc gacgtcattt
     6661 atttttgtgt attcagatta tcgttattat cttcgcaacc atcattatgg tatatggtgg
     6721 tgggctatta acgatggata cattcgattc aggtcagacc tcgcccgctt taggctggca
     6781 aatgggatat atctatatga gcatcc
//
LOCUS       DAPHJE010000186         6645 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPHJE010000186 DAPHJE010000000
VERSION     DAPHJE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.188"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..567
                     /gene="ftnA"
                     /locus_tag="RH222_004187"
     CDS             70..567
                     /gene="ftnA"
                     /locus_tag="RH222_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV3799113.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(605..844)
                     /locus_tag="RH222_004188"
     CDS             complement(605..844)
                     /locus_tag="RH222_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV3799114.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1034..2245
                     /gene="tyrP"
                     /locus_tag="RH222_004189"
     CDS             1034..2245
                     /gene="tyrP"
                     /locus_tag="RH222_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV3799115.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2307..2972)
                     /gene="yecA"
                     /locus_tag="RH222_004190"
     CDS             complement(2307..2972)
                     /gene="yecA"
                     /locus_tag="RH222_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV3799116.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3514..5283)
                     /locus_tag="RH222_004191"
     CDS             complement(3514..5283)
                     /locus_tag="RH222_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV3799117.1"
                     /translation="MLKFSGTFQELSGKLASLNGEWDDSQPNKKVLRLNGGVMNWFES
                     TGSISFQGREPGKSALESEVPKLLYPTEPMAIRPQSSALSPDALIKSQGNDEKDSSVE
                     RQYLTTGINDGELVIGIVSAVGTEYKRVTEPLIDRLKGFGYSVKEIRVSSCLPSTSQT
                     DEYERIRHYMQLGDSLRKSMGNNAILAAGVAKKISELRSPTDTKRAYIVNSLKHPGEV
                     EFLRKVYGGGFYLIGIHADEKRRHQHLTDDKGMTQSQANDLIRIDEDESIDHGQKTRD
                     TYHLADFFLNLGSNNDQVKNRLQRFLELIFSHPYKNPTFDEFAMFMAFNSSVRSGDLS
                     RQVGAVISRDTQIIATGANDVPKSGGGLYWAEVNEETGKVEDQPDGKDYTREGDSNKH
                     AQSVIIQEIATNLLNQGLVDSLHELDLKKALKESKISDLTEFGRVVHAEMDALLSCSR
                     AGIPTTGSTLYCTTFPCHNCAKHIIASGIKRVVYVEPYPKSRALDFHSESIHLRSELE
                     RTLKDNNNLVSFEPFIGVGPRRFLDLFSMSLGSGSKLRRKDKGGGILDWDKASAPIRT
                     PLLSKSYIEIEKAAADMWDECSL"
     gene            complement(5805..>6645)
                     /locus_tag="RH222_004192"
     CDS             complement(5805..>6645)
                     /locus_tag="RH222_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV3799118.1"
                     /translation="DYSTDNGTYSELQLFREVLVRKGFNELEQLPELLDKLLLTTSFN
                     ALYYGFAKEVKRHIKSTFTESLKSWIEEAGTTFRADLSLFLFYIWSNKIEFSSVEFNE
                     KAEATPGVPLVSFDEVKKYLAVCEHIYFDILVDRLATRLEHFNPNKFVTMYLVDAMDG
                     FQFEDFLVEVFQTMGYDVKETKRTQDQGADLFVTRFGKDMVIQAKNYSGSVGNSAVQQ
                     VISAKTFYGCDEAMVVTNSYFTRSAKELAESALVRLIDRDELQKYLDDYNQKIIEDFQ
                     SNS"
BASE COUNT         1794 a         1549 c         1368 g         1934 t
ORIGIN      
        1 ttgcaaataa taaaataaca aattatagtg acgccacagc aacaaatata acctttgtgg
       61 agcactatca tgctgaaacc agaaatgatt gaaaaactta atgagcagat gaacctggaa
      121 ctgtactctt cactgcttta tcagcaaatg agcgcctggt gcagctatca taccttcgaa
      181 ggtgctgccg cgttcctgcg ccgtcacgcc caggaagaga tgacgcatat gcagcgtctg
      241 tttgattacc tgactgatac cggcaattta ccgcgtatta ataccgttga atctccgttt
      301 gctgaatatt cctcacttga tgaattattc caggaaacct ataaacacga acaattaatc
      361 acgcagaaaa ttaacgaact ggctcatgct gcaatgacca atcaggacta cccaacattt
      421 aatttcctgc aatggtatgt ttctgagcag catgaagaag agaaactgtt caaatcgatt
      481 attgataaat taagcctggc aggcaaaagc ggcgaaggtc tgtattttat cgacaaagaa
      541 ctctctaccc tcgacacaca aaactaatgc tctcaggcgg caggttttct gccgccgttt
      601 caaatcagtg acggcaaatc ttactctgat cggtcgaaaa accttcttct gaaggtataa
      661 atttgccttt tgctgccaga aacgctacca gctctcccgc agtcatccct tctgccgagc
      721 aggtgtgaaa acgtgcctgt tcaccaaaac gcgctttaat tgctgcttcc agactggcat
      781 gcgtatattg ctcgcctgat tcaatcatca tatttaacac ttcatgaccg tgaatagagt
      841 ccatcgttcc tcctcaaaaa aagcctagcg tagcgattgc cgcttatgaa gactttgcgc
      901 cagcgcagaa ctaaacgctt ttattgtaca tttatattta cactatatgt aacgtcggtt
      961 tgacgaagca gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag
     1021 gacagaagaa agcgtgaaaa acagaactct gggaagtgtt tttatcgtgg cgggaaccac
     1081 aattggcgca ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt ttagcgttac
     1141 gttaatcttg ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga
     1201 ggtgtaccag catgttccgg cagataccgg tcttggcacg ctggcaaaac gctatctggg
     1261 acgctacggt cagtggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc
     1321 ggcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat
     1381 ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg
     1441 tgtcgggaca tcgctggtcg atttgtttaa ccgttttctg ttcagcgcca agattatttt
     1501 tctggtggta atgctggtac tactgctgcc gcatattcac aaagtgaatc ttttaaccct
     1561 gccgttgcaa caggggctgg ctctgtctgc aatcccggtg atttttacgt cgtttggttt
     1621 tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg
     1681 ggtgtttata atcggtagtg cgatccccct ggtggcatat attttctggc aggtggcgac
     1741 gcttggcagc attgattcaa caacctttat gggattgctg gctaatcatg ctggattaaa
     1801 cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca
     1861 tttatttgct gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga
     1921 ttatctggct gatttatttc agcgttcaaa taccgttggt ggacggttgc aaactggtgc
     1981 aattacgttt ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc
     2041 gctgggttac gccggtgtgg cgctggcggt actggcattg attatccctt cgctgttgac
     2101 ctggcaaagc agaaagcaca atcctcaggc gggttaccgg gtcaaaggtg gtcgtccggc
     2161 gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc
     2221 agggttgtta ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa
     2281 ttaacgctga cacccaattt ggcctcttaa tgcaggcagc actgcttaaa tttcttacca
     2341 ctaccgcacg ggcaaggatc gttacgcccc ggtttctctt ctgctttgat cggttgctga
     2401 acagcttttt cctgcggatg cgccatccag tacgcatgta gatcaagcgc cgccagtcga
     2461 atggcatcta cgctctcttc aaacgcttct ggcgacatct tttctacccg ctcgaagttt
     2521 tcctcagtac cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc gggcaacgtt
     2581 gaccagtcag aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc
     2641 agctcgctgc catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg
     2701 ttcagacgct ctgcggtatc ggccatatgt tgaaaagcca gattcataaa gcgcgtcatc
     2761 tctttctctg acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac
     2821 tgttccggtt caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc
     2881 gccacatcaa ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc
     2941 aactcacttt cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc
     3001 cagcacctat cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgctggtg
     3061 attaaaaatt aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg
     3121 gtttcacgac actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc
     3181 gggacttgaa cccgcacagc gcgaacgccg agggatttta aattctacgc aggatcaatg
     3241 tgttacatag ctattcattg caaaacgctg ctaaaattga ttactcattt tgctgtaatt
     3301 ggatgaaaat agataatcat ggatagcgtt gagcacagaa actgcacagg tactaaaaat
     3361 tatcgagagt gtcattgtgc ctattcctat ggaagacgag aaaaatcttc catgtttatg
     3421 ccaaaactcg gcacaccatg attattttta acatagccta gactgacttc cttgaaccca
     3481 gttcatcatt gcaacctttg aattatattt ctgtcaaagt gagcactcat cccacatatc
     3541 agcagcagct ttctcaattt ctatataaga ctttgataat aaaggtgtcc taataggggc
     3601 agatgcttta tcccaatcaa ggataccacc gcccttatcc ttgcggcgta atttagaacc
     3661 gctgccaaga ctcattgaaa aaagatccaa gaagcgacgt ggtccaaccc caataaacgg
     3721 ttcaaaactg actagattat tgttatcttt aagtgtgcgt tccaattcag atcttagatg
     3781 gatcgattca gaatggaaat ctaacgcacg acttttaggg taaggttcta cgtagacaac
     3841 gcgtttaatc ccactagcaa taatgtgttt tgcacagttg tggcatggga acgttgtaca
     3901 ataaagggtt gaacctgtag taggaatacc agcacgacta caagataaca atgcgtccat
     3961 ttcagcatgg acaacacggc caaattcagt caaatccgat attttacttt ccttaagtgc
     4021 ttttttaaga tcgagttcat gcaaactatc tactagccct tggtttaaaa gattcgtcgc
     4081 aatttcttgg ataatgactg actgcgcatg tttatttgag tccccttctc ttgtatagtc
     4141 ctttccgtct ggttgatctt caacttttcc agtttcttcg ttcacttcag cccagtaaag
     4201 gcccccacca gatttaggta catcattagc gccagttgca attatttgtg tatctcggct
     4261 aatcactgca ccgacttgac gtgataaatc accagaacga actgagctat taaaggccat
     4321 aaacatggca aactcatcaa atgtcgggtt tttataaggg tgtgaaaaaa ttaattctaa
     4381 aaatcgttgc aagcgattct taacctgatc attattactg ccaagattta aaaaaaagtc
     4441 agcaaggtga tatgtatctc tagttttttg tccatgatca attgattcat cttcatcaat
     4501 acgaattaga tcattagcct ggctttgagt cattccctta tcatcggtta aatgctgatg
     4561 ccgccgtttc tcatctgcat gaatacctat taagtagaat cctcccccat atacttttct
     4621 taagaactca acttccccag gatgtttaag tgagttaact atataggctc gctttgtatc
     4681 tgtcggggat cttaactctg aaatcttctt tgctacccct gcagctaata tggcattatt
     4741 acccatactt tttcgcaaag aatcacctaa ctgcatgtag tgtcgaatac gttcatactc
     4801 atctgtttgt gaagtacttg gcaaacaact cgacacccta atttccttaa ccgaataacc
     4861 gaaccctttc aaacggtcga ttaatggttc agtaactcgt ttatattctg ttccaacagc
     4921 actcacaatg ccaatgacaa gttcgccatc gttgatccca gtagtaagat attggcgctc
     4981 aactgaagaa tctttttcat cattgccttg agactttatc aacgcatcag gagataatgc
     5041 tgaggactgt ggccttatag ccattggttc tgttgggtat agtaatttag gtacttcaga
     5101 ttcaagggct gatttccccg gttctcttcc ctgaaaactg atagaacccg ttgattcaaa
     5161 ccagttcatc acaccgccat taagtcttaa aactttttta ttcggttgtg aatcatccca
     5221 ctcaccattg agtgaagcta attttccact aagttcttga aaagtaccag agaatttcaa
     5281 cattatagat ctcactctta gtttttcaga ttgagaagaa gcgtcactat tgcctgttca
     5341 ggctaatgca actatatatc aatagcttca cattacagtg accgaacatc tttttgccaa
     5401 gcgggaagaa atacgatatt gcctttttta gaatcacaag gaatactatc acccaccagc
     5461 atatacaaca ttctataata ctcccccggt aaaaactaac aatatcaaac cattacagaa
     5521 cacaaaaaag acagtttgca ctgttttcat tatgcgtgaa aagtaaaaac aacaattacc
     5581 acaaaatgtg gaaacccgta agtgtagata gtcatattca cactgtgata ccagaagatg
     5641 aaagaggttc atcatccgaa tgaacgatgg attcccctca cttcctagcc cgtgatgctc
     5701 agcagttata tgctatattg acgaaacatc acaggtaagt cccctgatac ggaaacttac
     5761 ctggttctat ccgtgtctaa atggtttcta aaaatgtttc aacgttatga gtttgactgg
     5821 aaatcttcga ttatcttttg attgtaatca tccagatact tttgcagttc atccctgtcg
     5881 atcagacgta ccaaggctga ttcagctaac tctttcgcag agcgtgtgaa gtaggaatta
     5941 gtcacaacca tcgcctcatc acaaccatag aacgttttag cagagatcac ctgctgcacc
     6001 gccgagttac ctacagaacc agaataattt ttcgcctgaa taaccatatc cttaccaaac
     6061 cgagtgacga agagatctgc cccctgatct tgcgttctct tggtttcttt gacgtcatag
     6121 cccatagtct ggaatacttc cacaaggaaa tcttcaaact ggaaaccgtc cattgcatca
     6181 actaaataca tggtgacgaa tttgttaggg ttaaaatgtt cgagccgtgt agctaatcta
     6241 tcaaccagaa tatcaaaata aatatgttcg cacacagcta agtatttttt aacttcatca
     6301 aatgaaacta aaggaacacc aggcgtagcc tccgcttttt cattgaactc tactgatgag
     6361 aactctattt tattgctcca tatatagaat agaaacaatg aaagatctgc cctaaaagtg
     6421 gtacccgctt cctcaatcca tgacttcaag gattcagtaa atgtggattt aatatgccgt
     6481 ttaacttcct tagcgaaacc ataataaaga gcgttaaatg acgtggtaag gagtaattta
     6541 tctaatagct caggtaactg ctcaagctca ttgaaccctt ttctgaccaa cacctcacgg
     6601 aaaagttgta attcggagta tgtaccatta tctgttgagt aatct
//
LOCUS       DAPHJE010000187         6614 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPHJE010000187 DAPHJE010000000
VERSION     DAPHJE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.189"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(308..4081)
                     /gene="rhsD"
                     /locus_tag="RH222_004193"
                     /pseudo
     CDS             complement(308..4081)
                     /gene="rhsD"
                     /locus_tag="RH222_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
     gene            complement(4149..6257)
                     /locus_tag="RH222_004194"
     CDS             complement(4149..6257)
                     /locus_tag="RH222_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV3799119.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1231 a         2076 c         1769 g         1538 t
ORIGIN      
        1 acgcctcaaa aatcatgttc gtccggctaa aacaattatt tgcaacagga agaggcccga
       61 ggttaatagt ttttagtttt ttattaaggt ctattgcctt attcatcata cattgttctt
      121 cttcatctgt tgtatctatt atccaaatcc aggtatctct gcggggttgc atctttttaa
      181 aataatcaga gacagaggct cccatctcat ccccagttcc cgaactcatt aatccatatc
      241 gatttgccgc cgcagcatgc ccaaaaggat tatcaggaac ggggccgtta cctattatca
      301 tagtactaag ccctaatgga tcaacgtgtt gcactggatt cagcggatac gcatacagac
      361 tccatccccc cgccagccca atcgggtcct gggtgatata ccgcccctgc aacggatcgt
      421 agtaccggtg acggttataa tccagccccg actcctcatc gtactgctgc cctggcagac
      481 ggtatggctg atacaggtaa tacgggttct cttcattcag ctggttgccc cattcatcgt
      541 attctgcact ccacacagta ttgccgtctt cgctgatgag cgccagcggc aggccccggt
      601 ggtcgcagtg gtaaaaatga acttttcgcg ccggtgtgta ttccggctcc acctgtctgg
      661 ccagttgctc caccgtcagc ccgcactgcg caagccacac ccggctttca ctgctcacgc
      721 ggtctgcccg gatttcttcc tccagtctgt ccagcagccg caccagttca gccggaaata
      781 ccacgccgtg cccgtcctcg ctcccttcct gctgcagctt ctccgccagg ctgcggcgct
      841 gcgctttctc ccgctcaccg ttgtctgttt cgatgcggat gagcggcgcg aagctccccg
      901 gctgatatac cgtctggata cgcgtggtgt cggtctgtac cgtcgtcagc cggtcgccgt
      961 cccacccgta ccacgtcacc tccggtttac gcgacagcga catccacccc gtcaggtcac
     1021 gctcccgccg ccatacccgt ttcgccattc gccgtcccag cgggtcgtag aggtagcggc
     1081 tctcgaccag tggctcgcca tgctgtatcc gcgtgtagaa caccagacgg tgctggctgt
     1141 cgtagtggta gtggtgggtc cgctcgtcgt ccgtccggat aacacccgcc gggataaggt
     1201 ccgtcttctc cgtcagtctg ccgtattcat catagtggta aacatagtgc gcatcctctg
     1261 cgatgcggtt atccggccac gccgtgaggg tactgtccgg gtgcagctcc gggtccggca
     1321 ggcggtttcc cgccgggtcg gtggcatacg ggatgcggat gtccaggtcc ggtgcgaggg
     1381 tacgcacgct ctccagcctg cccgtggcgc tgtagccgta ttcccgcgtc tgtcgcgggc
     1441 cgctgatgcg caccaggtcg ccgttgtcat tccacccgta gtcacggtca tacaccaggc
     1501 tgttcaggtg ctggctctgt aactggcctg cgggggtgta tgtgctggtc agtttatatg
     1561 cggcattact gcctgccatg ctgccaaagc tgcgcaccgt ctcccggtgc atgcggtcgc
     1621 gcgtgtactc cagcagcggc gtgtcgccaa gcttcatgcc cgccagataa ccgctgccgt
     1681 aggtcagcca ctccaccggc ggcaggctgt ccggcgtgac gcggtttgcc agcccctgtt
     1741 cgttgtaggc gtgtgttgtc tcatgctgcc acagcagttc ccccgtctcc gggttctcca
     1801 ccgtctgccg ttcgccggtc aggcggccct tatcgtcgta gccgtagtgg acagcgacac
     1861 ggtggccttc gctcaggtgg ctgatgtctg tcagccagcc gtggtcatca tactgccact
     1921 gctctgccgg ttcgccgttc accgtgcggt gagtgatacg gtccgattca tcgtagtacc
     1981 agagggtgac aagcccctca tcttcactct gcgtgagttt tccggtcagg tcataatgat
     2041 aacgttgcgt ccgcccgtca aagccgccct gctgtaccaa ccggtccagc gcatcgtagc
     2101 tgaagtcgct gtggctgccg ttctcgttag tcagactgat gacacgtccg gcagcatcgt
     2161 actccatact gcgcgtcagc ccgccctgcg tggtgctgac cgcctttccc cacgcatcgt
     2221 actgtgtctc gctccggttg ccgtccgggg tgataacggc agtcaggtcg cctgcggcgt
     2281 tgtactcata ctgcgtttca cggccctgtg cgtctttcac cgaggttaac cggccacggt
     2341 tgtcatagtg gcggtaaagg ctgatacctt cctcgcggtg aaccgccgtc atctggccga
     2401 agcggtcgta ttcatagcgg gtctggtagc ccgagcagtc agtgaacgcc agcaactgcc
     2461 cgtagcggct ccacgtcatc tgccgggtgc tgcccgtcgc atccgttgtc gtcgccggta
     2521 actcactgtg cgcgtcatcg tagcggtagc gtactgtctc cccgctgcgc gatgtctccg
     2581 ataccagcct gcccggttca tcatattccc ggcggctctc cagcccgtcc ggggacacca
     2641 ccgccgtcag ctggttcccg tcgttatagt aaaatttcgt ctcccgcccg tccggcgtgg
     2701 tgatgtccgt gatatcgcct gacaccacat tcagaccgta ctctgtccgc cgtcccgccg
     2761 catccgtctg cgccgtgagc cttcctgccg cgtcataccc gctgtgcgtg acgctgccgt
     2821 ccgccagttc ttttttcacc acccgcttca gcccggcccc gccttctgta tgcagcacct
     2881 cacgccggtt caggctgtcc gtgacggtga tgcgatcctg ctcatactgg tagcggtaac
     2941 tcaggcctgc cgggttaagt tgctccacca cccgccccgc atcgtcgtag cggtagcgca
     3001 tctccggcct gcctgcataa cggtgcgcca ccatccggcc cgggtgctgc gcgtcatacg
     3061 tgaaagcgcg cacctgcgta ttgctgcggt catataccgc cagcagttca ccggcttccg
     3121 tatatgtgta ccgcaccagt ggcgcagcgg gcaggctctc cgggtatgcc gggtcgtgca
     3181 tcagccacac cgccgaaagg cggatacccc tgtcggggcc gtattcggta ccgggcagtg
     3241 tgtcggggaa cgctgaggct gagagagggc gggaactgtc agaagaagat agcgaagagg
     3301 tgcgggcctc ttccgcacgc tgcgcctgcg tggtcagcac cagacggaac tcccgcccgg
     3361 caccgtccgt cacgccggtg atttccccgg tcaggtcacc ggcggcctca cgccggtacg
     3421 tcagcgtccg cccgaagcgg tccgccagcc ccgtcagctc ccggtacggc ggcagcggcg
     3481 ctggcagcac gtcctcagca cccggcaccc gctctgacca gccgagtatc caccacggcc
     3541 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggcggcagcg
     3601 ccccccacag ccgcgccagc gtatggccgt ccggctgtgc tgccttacca ccgcgcacca
     3661 gccacattga ctcactgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
     3721 tgctccgccc gccgttgtcg ttgagtatca gtccgtcatc acgtagctgt aagcggatat
     3781 cagaaggcgc tttccagccg gggccgaaaa cgcccaccgg tgcaggcgtc ttcgtccggt
     3841 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
     3901 cgccgggcag caccttcgcc ccaagcagcg gatttaccgg gttgcccgaa gtcatcccgc
     3961 ccggacacac cgagcacgcc acgccggtgg gcgcgccaat tcttacacct gccgaaccct
     4021 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
     4081 tgattcaaat tccttcttct gtttattttt aatgctggtt tgttactgaa ttccgaacag
     4141 gtacgggatc agtactctct catatccggc gtatcctgct tttcatcagg ggtcttcggt
     4201 ggggatttcg aggctgcaca gttccagtta atcaacagtg agtcaccatt aacctgctcc
     4261 ttaccctgca aatgaatttt tgtgccgttg agaaaaattt gtccgtcctt cgtcagcacc
     4321 agccttgcct taccacagca gagctccaga tgctcaccgg cggagtaaat cgcggtctgc
     4381 ccggtatcat cctttttcgt tttaccaacg gtgaaggtaa cgttatttcc gactttgacg
     4441 tcataaccca gcccgaccct caacgattta tgcaaaccga tctgcgagga ctgcatcagt
     4501 gccaccgagg tgttcataat gccacctacc gtcgtctgat acgccacgcc cacggtcagc
     4561 gcacgaacaa gtccaatcgt ttccacctga acagaaccca ctttaattat ctggttactg
     4621 cccaccgtct ctatctggtt aacacccacc gtctgtatct gattgttggt aaccgcaatc
     4681 atctgattgt tgccaatggt ttcagcatgg ttgtttatca catcagtggt gcggttattc
     4741 agcacctcgg tgttcatgtt cttctgcgcg tggatgtaga cctgttcttt ccctgtcgca
     4801 tcgtcaaact tcagttcatt aaacccgctg cccttatagg ttttcgaacg aatggtcatc
     4861 tgcgtcttcg tccccggcag gctgccgggt gtgcggtttt cctggtggta ggtacgcccc
     4921 ataataatcg gctggtccgg gtcgccgttg aggaagtcca caatcacctc ctgacccact
     4981 cgcggtatcg ccaggttgcc aaagccggtg cctgcccacg cctgtgccac acggatccag
     5041 catgaactgt cctggtttga cgggttatag cggtcccagt taaatttcac ccgcacgcgg
     5101 ccatgttcat cacagaagat ttcctcgcct gccggtcccg tcacgacggc gctctgcggg
     5161 ccgtccacca gcggtttcag caacggctgt ggccgccatg tccggtctgc cggtattacc
     5221 gcaaagtggt tattcagcgt ggtacctgaa ccactgcgtc ccggcaccgc ctgcggctgc
     5281 tcgccgtgca gttcacttgc caccacctgc cattcccggt tcaggttcgc ctgcggatgc
     5341 cccgtcagcg caattcgccg tcccggccat atctccggag aacggcttgt tccgcgcgcc
     5401 acttctgcgt tgtttcgcca gccatccatc tgccagcggg caaagttctg cccgtgggca
     5461 cccttgaaac gtccggggta gtcatacact tcatactgtg tgcgctggta atcctggtac
     5521 tggccttcct gatcaaaacg ccctgcccag ccgggtcgtt taaaggtgta gtccttggtc
     5581 accacggaag aagggcggat ttgtgcgctg tagcggaact ggctgatgca gagggtgctc
     5641 acctcggtac gggtgttcgg attccagggg atctcaaagg actccggcag ataacgcacg
     5701 gtgtcgcaca ggaccaggct ctggtcggta cttttttgcg cgtgctcctc ataaaagaag
     5761 atgccttcct ccgccgccat ccggcacagg aaatcgtaat cggtttcgcc gtactggaca
     5821 caaaactcac gggaaggatg cggctcgctg aacagcggac tccactcggt cacaccgttt
     5881 tccttcagga tcgtagcgag gatgctttcg atgtcctcat tctggaagat acggaagttc
     5941 tggcgcagcc ctgtgcgcca cagcggcggg cacaccttca tgctgtacag catctggttt
     6001 ttgtcgttct cccccagttc aaaccaggtc accacacctt tcacccggcg ctgtacgtca
     6061 tcgccctgcc ataccgtcag gtaggccatt ttgtccagca cctgcgcaaa ttcaagggaa
     6121 agaaactgct gactgaccag agaaaggtcg agggaaaaaa gtgaagagag tgactgggtg
     6181 agatgaaagg aaaccaccgc aaacgcgtcc ggtggcaggc cgtccacttc aagtgtgaaa
     6241 cgtaatccgg ttgacatagc aacctccgtg aaaatgaata ataatccgca catattgcat
     6301 gtacaacatg tcgggcggat gagataagcg agtcgacatc tggcgttacc ggggaatatc
     6361 agaaaaatgt catggttatg tgatgattta gtctgggatt ttacttcaga gaaacgggtg
     6421 tctgtttagt caacaaaata atcacaaacg caacggatgt gttctcgacg ttattcacga
     6481 caggctcgta cattacagcg agtaataaaa aacctgatat gcaaagtcaa accaatattc
     6541 ataatgtctt tttattcaca acaataaata attcaataac aatcattgaa atgaatgatg
     6601 atttgtataa ggaa
//
LOCUS       DAPHJE010000188         6553 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPHJE010000188 DAPHJE010000000
VERSION     DAPHJE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.190"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            104..346
                     /locus_tag="RH222_004195"
     CDS             104..346
                     /locus_tag="RH222_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799120.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            460..1098
                     /locus_tag="RH222_004196"
     CDS             460..1098
                     /locus_tag="RH222_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV3799121.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1095..1821
                     /locus_tag="RH222_004197"
                     /pseudo
     CDS             1095..1821
                     /locus_tag="RH222_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            1821..3917
                     /locus_tag="RH222_004198"
     CDS             1821..3917
                     /locus_tag="RH222_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV3799122.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3964..4242)
                     /locus_tag="RH222_004199"
     CDS             complement(3964..4242)
                     /locus_tag="RH222_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799123.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4243..4386
                     /locus_tag="RH222_004200"
     CDS             4243..4386
                     /locus_tag="RH222_004200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799124.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4512..4922
                     /gene="psiE"
                     /locus_tag="RH222_004201"
     CDS             4512..4922
                     /gene="psiE"
                     /locus_tag="RH222_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV3799125.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4966..6441)
                     /gene="xylE"
                     /locus_tag="RH222_004202"
     CDS             complement(4966..6441)
                     /gene="xylE"
                     /locus_tag="RH222_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV3799126.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1613 a         1671 c         1777 g         1492 t
ORIGIN      
        1 ctaattcccc gcgccgtaat tcgcatgctt tagttgtgta tactcgatcc cgcccgaaat
       61 gtttttgggt aaatctccat tcattcaatg aagggaaatt gttatgaaaa aagttctgta
      121 tggcattttt gccatatctg cgcttgcggc gacttctgcg tgggctgcac ctgtacaggt
      181 gggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg gggagttcct ccgcgaccag
      241 cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg gcggtgatgg
      301 ttctaacacc gggaccacca caaccacgac caccagtacc cagtaataaa gtatgtatcc
      361 ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat gaagggattt accataacca
      421 cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc tgcactcatt
      481 cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg caattcactg
      541 tgggacagtc tgttcggcac gccaggcgta cagctgacgg acgatgatat tcaaaatatg
      601 ccctacgcca gccagtacat gcagcttaat ggcgggccgc aattgtttgt ggtgctggcc
      661 ttcgctgaag acggacaaca aaaatgggtc actcaggatc aagccactct tgtcacacaa
      721 catggtcgtc tggtgaagac tttgcttggc ggcgacaacc tgattgaagt gaataacctt
      781 gccgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacatg gacgcgcacg
      841 atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt cttcaaatgg
      901 gatggcaccg ataccgtcaa ggtcggcagc gatgaaaccc cggttcgcgt gctggacgaa
      961 gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag cgaagggcaa
     1021 attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac cactctcatc
     1081 aaggcggcaa aacaatgatt aaacaaacta ttgtcgcatt gattttgagt gtgggggcgt
     1141 catcggtctt cgcggcggga accgtcaagg tattcagcaa tggcagcagt gaggccaaaa
     1201 cgctgacggg cgcagagtat ttaatcgatc tggtaggcca gccgcggctg gcgaatagct
     1261 ggtggcctgg tgcggtgatt agcgaagagc tggcaacggc ggcagcagca ggcgttgctg
     1321 acacggctgg cagaactggc tgcagattcc agcgccgacg atgccgccgc gattaacgcc
     1381 ttacgccagc aaattcaggc gttgaaagtg acgggcaggc aaaaaatcaa tcttgatcct
     1441 gatatcgtcc gcgttgccga acgcggtaac ccaccgttgc agggcaacta cacgctgtgg
     1501 gtcgggccac caccgtccac ggtcacgctg ttcgggctta tcagccgtcc tggcaagcag
     1561 ccattcactc caggtcgcga cgcggcgagc tatctctctg accagagcct gctcagcggt
     1621 gcggatcgca gctacgcgtg ggtcgtttac ccggacggac gcacacaaaa agcgccggtg
     1681 gcttactgga acaagcgtca cgtagagccg atgcccggca gcattattta tgttggcctc
     1741 gcggactccg tctggagtga gacgcctgat gcccttaacg ccgacattct tcagactctg
     1801 acgcagcgga taccccaata atgaaaaaaa gacatctgct tagcttactg gcgctgggca
     1861 ttagcacagc ttgctacggc gaaacatatc ctgcgcccat tggtccgtcg cagtcggatt
     1921 tcggtggcgt aggattatta caaacgccca ccgcgcgcat ggcgcgggaa ggggagttga
     1981 gcctgaacta tcgagataac gatcagtacc gttattactc agcttcagtg caactcttcc
     2041 cgtggctgga aacaacgctg cgctacaccg acgtgcgcac ccggcagtac agcagcgtcg
     2101 aagcgttctc tggcgatcaa acgtataaag ataaagcctt cgatctcaaa ctgcgtttgt
     2161 gggaagagag ttactggctg ccgcaagtgg cggttggtgc gcgggatatc ggcggtacgg
     2221 ggctgtttga tgcggaatat cttgttgcca gcaaagcctg ggggccgttc gattttacgc
     2281 tcggcctggg ctggggatat ctgggcacca gcggtaatgt gaaaaatccg ctctgttcag
     2341 ccagtgataa atattgctat cgcgataaca gctacaaaca ggcgggatct atcgacggta
     2401 gccagatgtt ccacggtcct gcctcactgt ttggcggcgt ggaataccag acgccctggc
     2461 aaccgctgcg cctgaaactg gagtatgaag gcaataatta tcagcaggat tttgccggga
     2521 agctggagca aaaaagtaag tttaacgtcg gtgcgattta tcgcgttacc gattgggccg
     2581 acgttaacct tagctatgaa cgtggcaaca cctttatgtt tggcgttacg ttgcgcacca
     2641 actttaacga tctgcgcccg tcttacaacg ataacgcccg cccgcaatat caaccgcagc
     2701 cgcaggatgc cattttgcag cattcggtgg tggcgaatca gttaacgctg ttgaaataca
     2761 atgccggact tgccgatcca cagatccagg cgaaaggcga tacgctgtat gttaccggcg
     2821 agcaggtgaa atatcgtgat tcgcgcgaag ggatcatccg tgctaatcgg atcgtgatga
     2881 acgatctgcc ggatgggatc aaaacgatcc gcattacgga aaatcgcctt aacatgccgc
     2941 aggtgacgac ggaaacggat gtcgccagcc tgaaaaatca tctcgccgga gagccgttgg
     3001 gccacgaaac gaagctggcg caaaaacgcg tcgagccagt ggttccgaag tccaccgagc
     3061 agggctggta tatcgacaaa tcgcgctttg atttccatat cgatccggtg ctgaaccagt
     3121 cggtcggtgg cccggaaaac ttttacatgt atcagctggg cgtgatggga acggcagatt
     3181 tgtggctgac ggaccatctg ctgaccaccg gcagcctgtt tgcaaatctt gccaacaact
     3241 acgacaagtt taactacacc aatccgccgc aggactcgca cttaccgcgc gtgcgtaccc
     3301 atgtgcgcga gtatgtgcag aacgatgtct atgtgaataa cctgcaagcc aactacttcc
     3361 agcatctggg caacggcttc tacggtcagg tctacggcgg ctatctcgaa accatgtttg
     3421 gcggtgcggg ggctgaggtg ttgtatcgcc cgctggacag caactgggcg tttggtctgg
     3481 atgccaacta cgttaaacag cgcgactggc gtagtgcaaa agatatgatg aaattcaccg
     3541 actacagcgt gaaaaccgga catctgaccg cctactggac gccatctttc gctcaggatg
     3601 tgttagttaa agccagcgtc gggcagtatc tggcagggga taaaggcggc acgctggaga
     3661 tcgccaaacg ctttgatagc ggtgtggtgg tgggtggcta tgccacgatc acgaatgttt
     3721 cgaaagagga gtacggcgaa ggggacttca ccaaaggcgt gtatgtctcg gtaccgttgg
     3781 atctcttctc gtctggcccg acacgcagcc gtgcggcaat tggctggacg ccgctgacgc
     3841 gtgacggtgg tcagcaactt gggcgtaagt tccagttgta tgacatgacc agcgaccgta
     3901 gcgtaaattt ccgctaagtc atgggaaagg tgccagtttt cgcactcact ggcaccttca
     3961 ttcttaatgg cctgtcgagc catcaggaac gcagttcacc cccggtttgc gatctggtgt
     4021 gtaaaggcga aaacattctg tcttcctgtc cgtcgatatt ggttgcaggt ctggttgcct
     4081 gagcgactgg gccatcattg ggctggagac attgctttgc ggtaggtcgg ctttatcagc
     4141 agtaccgagc ggaccagcat aagcaggaag aacagagact gataacatca aagcagcaaa
     4201 ataaggcttc atttttacca cctttatcag gttacgtttc atttgttcca gaggaacatt
     4261 gtcgattttt cgcgcattgc tggtggctgg gaatcacctg aatgggtgat ttttgaatta
     4321 ccggctttgg tgcggtttgt cttgccggat gcgccgccag gcgcggctta tccggcctac
     4381 gggtaggtat atccggcttt ggtggaggcg cgctccaaat ccaggttgaa caaaacatac
     4441 acaaaaaata tagatctccg tcacattttt gcgttataca ggaagctcgc cactgtgaag
     4501 gaggtactgc tatgacgtca ctctctcgtc cgcgcgtgga gtttatctcc accattttgc
     4561 agaccgtact caatcttggc ctgttgtgcc tcggcctgat tttggttgtc ttcctcggca
     4621 aagaaacggt gcatctggct gatgtgctat tcgcgccaga acaaaccagc aaatatgagc
     4681 tggtagaagg actggtggtt tactttctct atttcgaatt tatcgcgctg attgtgaagt
     4741 actttcagtc cggttttcac ttcccgctac gttactttgt ctatattggg atcaccgcca
     4801 ttgtgcgctt gatcatcgtc gatcataaat cgccactgga tgtgttgatc tactcggctg
     4861 cgatcctgtt actggtgatc accctgtggc tgtgtaattc gaaacggctg aagcgggagt
     4921 aaaaagacag cacgccgaac tggcgcggcg tgctggacag gaagattaca gcgtagcagt
     4981 ttgttgtgtt ttcttcgttt ccggttccca gagggcttcc agctcctcaa gggttttacc
     5041 tttggtttcc gggacaaatt tccacataaa tagtgctgcc agaacgccca tacacccgta
     5101 aatccagtag gagaaaccgt tgtggaaatg ggccaccagc caggagtttt tgtccatcat
     5161 cgggaaggtc caggagacga agtagttcgc cagccactgg gccgccaccg cgattgccag
     5221 cgctttacca cgaatagcat tcgggaagat ttccgacagc agtacccagc ataccggacc
     5281 ccaggacatg gcaaaggcgg caacatagaa cagcatcgac agaagcgcca caatacccgg
     5341 tgcctgagtg taaaacgcgg taccgaggct aaacataccg attgccattc cgagtgcgcc
     5401 gataatttgc agtggcttac gaccaaattt atccaccgtc ataattgcca gaacggtgaa
     5461 ggtgaggttg ataactccga caataatggt ctgcaacagc gcgatatccg tgctggcccc
     5521 cagcgttttg aacacttccg gcgcgtagta cagcaccaca ttgatgccga caaattgctg
     5581 gaggatggag agcataacgc cgatgacaat cacgcctacg ccaaacatca gcagacgacc
     5641 accggttttg cggccatgat ccagagagtg tttaatttcc tgtactgcct gagttgcaag
     5701 ggtgttgccc ataattttgc gcaggatacc ttccgcctgt tcttgcttgc cgcgtgacat
     5761 cagccagcga ggactttctg gcacggtata cagcagcatt aagaacagca gtgcagggat
     5821 acattccgag gcaaacatat aacgccagcc gtcagtattc agccagctgg cgtcgccgga
     5881 acgggcaata aaatagttta cgcagtaaac taaaagctgc ccgaaaataa tcgcaaactg
     5941 gttaaaagag accagtttcc cgcgaatatg agcaggagca agttccgcaa tatacattgg
     6001 tgaaagcatt gaggctaaac caacgccaat accgccaata atgcgataaa taacaaattc
     6061 cgggacataa cctgccagat aaacaggcac tgtgttgtcc gggtttatag aggtaaaacc
     6121 aagttctggc caggcagaac ctacaccaga aataaaaaac aggacagcag caatcttaag
     6181 tgaatcacga cgaccgaagc ggctactgca ataaccaccg agggcaccgc cgatgatgca
     6241 accaatcaga gcgctggcca cgcaaaaccc taacagggag ttggcagcgg attcacttaa
     6301 gttttgtgga gcaacaaaga cggtattgag tgactcaaca gtaccggaaa taacggcggt
     6361 gtcgtagcca aataataaac cacctaatgt agcgactaag gtaatcgaaa atatataact
     6421 ggaattatac tgggtattca ttcagacctg ccttagacca ttctgatctt ttatcagtgc
     6481 attgatgttg gaattgtcag gcagttaagg caattatcat tttttacaat gcgtttacgt
     6541 aatgtctttt ctg
//
LOCUS       DAPHJE010000189         6370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPHJE010000189 DAPHJE010000000
VERSION     DAPHJE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.191"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..342
                     /locus_tag="RH222_004203"
     CDS             <1..342
                     /locus_tag="RH222_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3799127.1"
                     /translation="TLHYVWAREFGECKGKKHYHLMLLVNRDTWCRAGDYRAPESLAG
                     MIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEYTKAHC
                     TGERNFGCSRS"
     gene            602..1003
                     /locus_tag="RH222_004204"
     CDS             602..1003
                     /locus_tag="RH222_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799128.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            991..1425
                     /locus_tag="RH222_004205"
     CDS             991..1425
                     /locus_tag="RH222_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV3799129.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            1780..2160
                     /locus_tag="RH222_004206"
     CDS             1780..2160
                     /locus_tag="RH222_004206"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3799130.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            2157..2504
                     /gene="tnpB"
                     /locus_tag="RH222_004207"
     CDS             2157..2504
                     /gene="tnpB"
                     /locus_tag="RH222_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3799131.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            2554..3939
                     /locus_tag="RH222_004208"
     CDS             2554..3939
                     /locus_tag="RH222_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV3799132.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(4178..5536)
                     /locus_tag="RH222_004209"
     CDS             complement(4178..5536)
                     /locus_tag="RH222_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HDV3799133.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKVGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            5818..5949
                     /locus_tag="RH222_004210"
                     /pseudo
     CDS             5818..5949
                     /locus_tag="RH222_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1481 a         1675 c         1728 g         1486 t
ORIGIN      
        1 accctgcatt acgtctgggc ccgggagttt ggggagtgca aaggtaaaaa acactatcac
       61 ctgatgctgc tggtcaaccg ggatacctgg tgtcgtgccg gtgattaccg cgctccggag
      121 tctctggccg ggatgattaa acaggcgtgg tgcagtgccc tgggagtgga tgtcgggtgc
      181 catgccacgc tggtgcattt tccggcctgg ccggcggtgt ggctggcgcg taatgatgac
      241 accggctttc agcaagtgct ggaacgtgct gactatctgg cgaaggagta taccaaagct
      301 cactgcaccg gtgagcgcaa ttttggctgt agccggagtt gagcacgact ggcgcgccaa
      361 tatccggcaa accaaaagta tgggttgttg tctggcggca acgatgtatc cactcgccca
      421 ctgactgttt tttacgtcag tgttccattg tgtgacaacc acagtgctgt aacagcaacc
      481 agcctgcttt acccaaactc cagtccagat tttaactgaa ccgccattca caccctgacc
      541 acgctgcccg taccgggacg gttgttgcct gcgtgtcgtc tggcggctaa ggaatatgac
      601 tatgtacgca aaatccttta tcgctcttga tggcaacgga cgtctgacgg gcgcccgtac
      661 tgcacaggcc gcaccttatg ctcactacac ctgccacttg tgtggcagtg cactcagata
      721 ccatccgcaa tacgacactg aacttccctg gtttgaacac actgacgaca ggctgacaga
      781 gcacggtcaa cagtgccctt atgtcaggcc ggagcgcaga gaaatacagt tgattaaacg
      841 tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt aaagccagct ggcactgcag
      901 acaatgtcac cacgattatt atggggagca gtactgcaca cactgccaga ccggaggatt
      961 cagtattccc cggacaactc aggaggaaat atgcgaattc tgaactgtta tatggcgaat
     1021 gacagcaaag gccattttgt tacggcgaaa gaagctgcga agcacaaccg acaggacgtt
     1081 ttgtgctgtg tgtcctgtgg atgcccgtta acacttcagc ggggcaatga cggacaacca
     1141 ccgtggtttg aacatgacca gatgactgtc gctgaaaaaa tcctgctgcg atgtacctgg
     1201 cttgacccgg cagagaaaga ggcccgtcgt ttgcatctgc agggcatgac ggttccggat
     1261 tatacggtga aggtgagaaa gtggttttgt gtgatgtgtg acgaagatta tgagggggaa
     1321 aagtgctgcc cacgctgcgg taccggggta tacagcaggg cgtgggggcg gcaggaggtg
     1381 ccgtcggaag atgccagggc tgataatccg ttacagaggc tgtaatggtt gcctccggag
     1441 cagtttgcgg tgatgccttt cccgggaatg gattgccagt gcggggactg tggagcttaa
     1501 ttccggtgtt ggtggccttt ccattgattt tatgccaaca gccccctgtg actgacaggc
     1561 tgcctcgtca ttccattcgt atctccagta acagggggtt gtattgtatt ttattgtacc
     1621 ggttcaggtg tgcgtttccc ggcgcgtctg caccggctta accaaattca acagggataa
     1681 aatagtggta agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt agacaaagtg
     1741 gtgtccacca aataagtagt gggaaccaaa gtgtcagata tgcagaaaaa tgtgactccc
     1801 ggcaggcgaa aaggctgccc taattatcct cccgaattta aacagctgct cgttgctgcc
     1861 tcctgtgaac ccgggatatc catctcaaaa cttgctcttg aaaatggcat taacgccaat
     1921 ctgttgttca aatggcgaca acaatggcgc gagggaaagc tgctattacc ttcttcagag
     1981 agcccccagc tacttcctgt gactctcgat gcagctgccg aacagccaga atcgctcgca
     2041 gaggacccgg aaaccctcag tatcagctgt gaggtaacgt tccggcacgg gacgctccgc
     2101 ttcaatggca atgtcagcga aaagctcctg actctgctga tacaggaact gaagcgatga
     2161 tcccgttacc ttccgggacc aaaatttggc tggttgccgg tatcaccgat atgagaaatg
     2221 gcttcaacgg cctggctgcg aaagtacaaa cggcgctgaa agacgatccc atgtccggcc
     2281 atgttttcat tttccggggc cgcagcggca gtcaggttaa actgctgtgg tccaccggtg
     2341 acggactgtg cctcctgacc aaacggctgg agcgtgggcg cttcgcctgg ccgtcagccc
     2401 gtgatggcaa agtgttcctt acgcaggcgc agctggcgat gctgctggaa ggtatcgact
     2461 ggcgacagcc caagcggctg ctgacctccc tgaccatgct gtaaatctct ttatcctggt
     2521 tgtcacagaa taagcccggt aaaatacggg cttatgaacg acatctcttc tgacgacatc
     2581 ttcctgctaa aacagcgcct ggccgaacag gaagcgctga tccacgccct gcaggaaaag
     2641 ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc agctggataa actccgccgg
     2701 atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat ggaagccgat
     2761 ctgaaccggc ttcagaaaga gagcgatacg ctgactggta gggtgtatga cccggcagta
     2821 cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc ctgaatcact accccgtgac
     2881 gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact gcggcggttc actgagctat
     2941 ctgggcgagg ataccgccga acagctggag ttgatgcgta gcgccttccg ggttatccgg
     3001 acggtacggg aaaaacatgc ctgtactcag tgcgatgcca tcgtgcaggc acctgcacct
     3061 tcgcggccca tcgagcgggg tatcgccgga ccggggctgc tggcccgcgt gctgacctcg
     3121 aagtatgcag agcacacccc gctgtattgc cagtcagaaa tatacggccg gcaaggtgtg
     3181 gagctgagcc gttcactgct gtcgggctgg gtggatgcat gctgccggct gctgtctccg
     3241 ctggaagagg cgcttcatgg ctatgtcatg actgacggca aactccatgc cgatgatacc
     3301 ccggtccagg tactgctgcc gggtaataag aagacgaaga ccgggcggtt gtgggcgtat
     3361 gttcgtgatg accgcaatgc cgggtcagcg ttggcacctg cagtgtggtt cgcttacagc
     3421 ccggacagaa aaggcatcca tccgcagact catcttgctt gcttcagcgg tgtgctgcaa
     3481 gcggatgcgt acgccgggtt caacgagctg tatcgcaatg gtgggataac ggaagctgcc
     3541 tgctgggctc atgcccgccg aaagatccac gatgtgcacg tccgcatccc gtcagcactg
     3601 acggaagaag ccctagagca gatcggtcag ttgtacgcca tagaggcgga tataagggga
     3661 atgccggcag agcagcggct tgctgaacgt cagcgaaaaa cgaaaccgct gttgaaatcc
     3721 ctggaaagct ggttgcgtga aaagatgaag accctgttct tcggctctgg ccatggtggt
     3781 gagcggggag cgctactgta cagcctgatc gggacgtgca aactgaatga cgtggatcca
     3841 gaaagctacc ttcgccatgt gcctggcgtc atagcagact ggccggtcaa ccgggtcagc
     3901 gaactgcttc cgtggcgcat agcactgcca gctgaataac acatccccgt caatacggcc
     3961 ctcgctgtac gcttactttt gttcggactg cttatggcct ttttccactg gtgtagcagg
     4021 aaagttaacc ggctgccacc atgagatgac gtgtaaaccg tgcaggcatg ttttcacgtg
     4081 gtgacgcttt cattgggggt ctacgccgga accccaatat tgttcacata catgaatttg
     4141 aaaaactaag cgatactgct gtccgtacct tataaactta tttcaacgct tccggcagcg
     4201 tcacaatcca cagttcactg tcagactccg gtttctccag tggcttcact ctgagggtcg
     4261 tttcaacctg cttgtcgtca agcgtcagct ttccttccgc gttcacccga taatccttga
     4321 atgtcttgcc ctcgaccgga tgttgcatcg ccaccttcac gccaaagtcg ttatcgttcg
     4381 ccaccgccag cgttttgctg tcgatcagcg ccaacccttc agccttctcc tgctgccagc
     4441 ccaaggagcg cagatcaaca acctgcgttt tctgcgcaag ggtaatgccg cgctgtgaca
     4501 gggttttctc atcatcaaac tccggatact cacccggctt gtcgaagcct gacaggtcgc
     4561 tcgccttatt gagatcgacc tcgtaaatca ggttacgcat tctgttgttt ttatctcttc
     4621 cctgttcgat cagcaggatg tgctggttat cgagcgccac gatgtctccg attttagcgt
     4681 cgctgttttt gctgtaggcc gcgctgtcga taggataacc gtacatcgcg gtttttccgc
     4741 ttgccggatc gaagctcacc agacgcgtaa acagcgcctt tttcttgctc ttagcgtcga
     4801 tatccagcgt actttgcacg gcgacaataa tacgcccgtc cggcatacgg gtgaggcctt
     4861 caaagccgcg gttcgcctga cgccatttga ggatatttgg caaaccgccc gcaatcgctt
     4921 tttccccttc cgccgcctgc ggaccgtgga ttgccaggat tttcccttta ctgtcgatgt
     4981 taatcaggaa cgggccatac tcatcgcaca gccagtaacc tactttacca tccggagtga
     5041 tcccttccgt atccagcccg cgattatccc ccttcagtct atgtaacgtg tcgctgaagg
     5101 ccacttcatt ggtggagcca atcacgtcgc ttgccagcgg cagaccgttg atcgcgcctt
     5161 tatcgtcatg cagaggacgg ggatcgatgg cctccgcttt gccgttttgt acacgaatcg
     5221 tcatcagcag cggcgcgaaa tctggcgtaa cgaaaatttt agtttcgttt ttcccctctt
     5281 tcggtgaatc cgcgttcgga ccgcgatcgg taatggttgc gaatgtcagg gcatcgccct
     5341 gtttaccggt gaacagcagg ccagaaccta tcccaaccgg aaggccgttc gggaatgcac
     5401 tggcaaatgc gccggcgtag ttcacgtgcg tgccttctgg aaaactgacg acatagcgct
     5461 cagccgtagg ttgtgccgcc agggcagaga aagagagcgt acaaccgatt agcacgggaa
     5521 taatttttct tttcatattg ttagcatcca taaagtgaag atcacactac accataaaga
     5581 agaaacgagt caggaaaatg acatcgaatg gcaacatgcg cgggcctgag gccaaagaaa
     5641 tgtaaaacac tgttgttatt tgtaacaacg gaaacattta cggaaattat ttgtctttct
     5701 tcgtgatcaa aaatgctgtg tcttgttgga ttggagtgat gagggctgcg tttaggaaac
     5761 gccctatatt gtacggttgc attatcgcgg taggggtaat gcacgtgtca gtatgatgag
     5821 ctgaccacac tcacgtgggt ggactggtat aacaatcgac gattgctgga aaggcagggc
     5881 catatcccac cggcagaagc agaaaaagct tattatgctt ccatcggaaa cgatgatctg
     5941 gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc acttagccgc
     6001 gtgtccatta ttgctggata agatcataac gatacaccgg gttaaacagg tggtcactgc
     6061 ttcactgaag ctgcgtgatt atgatggtca ggtagcagaa gctatgccca tgataccaaa
     6121 atgacgaaag ccgaaatgtc tgaaagcgtg tgtattacct gaaaatatga ccggctccgg
     6181 ggtacgtaac cgattcaaca aagccaatcg atattacaaa acgagaggga ctatatattt
     6241 cgttttcaga taagactcct tgagagatta tcttttatgt aaaatctcaa atatcacctg
     6301 atgtaatgtc aatgataata ttaagtgcgc tctgaacaac atctttcttg gtgtgattaa
     6361 catctgacaa
//
LOCUS       DAPHJE010000190         6275 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPHJE010000190 DAPHJE010000000
VERSION     DAPHJE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.192"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(213..1883)
                     /gene="betA"
                     /locus_tag="RH222_004211"
     CDS             complement(213..1883)
                     /gene="betA"
                     /locus_tag="RH222_004211"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV3799134.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1897..3369)
                     /gene="betB"
                     /locus_tag="RH222_004212"
     CDS             complement(1897..3369)
                     /gene="betB"
                     /locus_tag="RH222_004212"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV3799135.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSALQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3383..3970)
                     /gene="betI"
                     /locus_tag="RH222_004213"
     CDS             complement(3383..3970)
                     /gene="betI"
                     /locus_tag="RH222_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV3799136.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4099..6132
                     /gene="betT"
                     /locus_tag="RH222_004214"
     CDS             4099..6132
                     /gene="betT"
                     /locus_tag="RH222_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV3799137.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1289 a         1722 c         1737 g         1527 t
ORIGIN      
        1 aatatatcca gaaaataaaa tctgtcgcag ataaggttgt attaatagtc tgtatcagga
       61 atgttcgggt taaatatcag caaaaagccc gcatcatgaa tactggatat gaagcatgag
      121 agttacctca gtgtttatat aaggattcgg tccccctctc tggaacggta actctcaatc
      181 tgatcggttc ctgcgttagt tcacatcacg actcattttt tcgctctcac cggcatccca
      241 tttgccacaa aatatcccgc cgtgctcctc ggcagcgctt cctgtccacg aatcatatcc
      301 gctattttct cgccaatcat aattgtcgtg gcgttcaaat tcccggtgat aatctgcggc
      361 ataatcgacg catccaccac acgcaggcct tctaacccgt gtacgcggcc ttcgccgtca
      421 accacggaca tctcgtcgta acccattttg caggtaccgc acggatggaa ggcggtttcg
      481 gcgtggttac gcacgaactc atcgagctgt tcatccgtct ggcattcgac accggggctg
      541 atttcgcggc cacgatactg atccagcgcg ggttgatgca tgatctcgcg ggtgatgcga
      601 attgcgtcgc ggaactcctg ccagtcctgc tcgtgcgaca tgtagttaaa cagaatcgcc
      661 ggatgctggt gcgggtcgcg ggatttaatc cgcacatgcc cacggcttgg cgagcgcatt
      721 gagccgacgt ggcactggaa accgtgctct ttcactgcat tcgagccgtt atagttaatc
      781 gctactggca ggaaatggta ctgaatattc ggccacgcaa attcctcacg gctgcgaata
      841 aatccacctg cttcaaagtg gttgctggca ccaacgccag tgccgccaaa cagccactcc
      901 gcaccgattt tcggctggtt ccaccactgc agggcagggt agagggaaac cggttctttg
      961 cactcatatt gcagatacat ctccagatga tcctgaagat tttcgccgac gccgggtaat
     1021 tcatgcacca gcggaatatc aaactccgcc agcagttcag cgttgccgac gccggagcgt
     1081 tgcaggatct gcggtgaggc aatcgcgcct gcacataaca gcacttcttt gttggccgtt
     1141 gcgcgggttg ggatggtgct gtcgccttcc agccattcga cgcccaccgc gcgtttgccg
     1201 tcaaaaatga tgtgatcggt catagcgtga gtacgaatgg tcaggttagg acgcgatttg
     1261 gcctgatcga gatagccacg cgcggtgctg gcgcgacggc cctgcggcgt gacggtgcga
     1321 tccatcggac caaaaccttc ctgctgataa ccgttgagat cgtccgtgcg cgggtagccc
     1381 gcctgcacgc ccgcttcaat catcgcttca aacagcggat tgacgccggg tttggaggta
     1441 gtgacgctca ccgggccatc accgccgtga tagtcgtttt cacccatatc gcgagtctcg
     1501 gccttgcggt agtagggcag gcagtcgagg tagctccagt tctccagacc gggttcttgc
     1561 gcccagttat cgagatccag cgcattgcca cggatgtagc acatgccgtt gatcagcgac
     1621 gatccaccca gacctttacc gcgtccgcac tccatgcggc ggttattcat aaacggttca
     1681 ggttccgttt cataggccca gttgtagcgt ttaccctgta gcgggaatgc cagggcagcg
     1741 ggcatctggg tgcggaagtc aaagcgatag tccgggccgc ccgcttcaag cagcagcacg
     1801 gaggtattcg gatcttcagt cagacgggta gcgagaacgt tgccggctga gccggcacca
     1861 ataatgatgt agtcaaattg caaataaacc tcctggttag aatatggact ggaatttagc
     1921 catctcaacc tggatggact tcacctgggt gtaactctgg agcgtcatca cgccgttctc
     1981 gcgaccaatg ccggagtgtt tgtagccgcc aacgggcatc tctgccgggg attcgcccca
     2041 ggtgttgatc cagcaaatac ccgcttccag ctgatgaatg acgcgatgcg cgcggttcag
     2101 gtccgctgtc acgatgcccg ccgccaggcc gtagtcggta tcgttagcgc ggcgaatgac
     2161 ttcgtcttcc gactcgtagg tcagaatgga catcactggc ccgaagatct cttcacgcac
     2221 gatggtcata tcgtcgctgc aatcggtgaa cactgtcggt gcaacccatg cgccgttatc
     2281 gaagccatcg cctttcagta catcgccgcc gcacagtacg cgcgcgcctt cctctttgcc
     2341 tttggcgata tagcgcagca cgttatcgcg atgcgggaag ctgaccagcg ggccgaagtt
     2401 agtttgcgga tcgaaaacgt cgcccgcgcg aatgcgctca acgcgcgcca gaattttctg
     2461 ctcaaatgcg gctttgcatt tcgccggaac gaagacgcgg gtgccattgg tacacacctg
     2521 accggagctg aagaagtttg ccatcatggc gatatcggcg gcgagatcga gatccgcatc
     2581 atcgaaaacg atcagcggtg atttaccgcc cagttccatg gtcacttctt tcagggaaga
     2641 ggccgccgag ttagccatca cttttttgcc gctggcgaca ccgccggtaa atgacacttt
     2701 ggcaatgccc ggatgctcgg tcagatattg cccggtctcc gcgcccacgc ccggcaacac
     2761 gttaaatacg ccgtccggca ggcccgcttc gctgtaaatt tcagccagct ttaacgcggt
     2821 aagcggggta acttcgctcg gtttgaaaat cattgcgttg cctgccgcca gcgccggggc
     2881 ggatttccac agggcaatct ggatcgggta gttccatgcg ccaatccctg ccactacgcc
     2941 cagcggttcg cggcgggtat acacaaagga cgtttcacgc aacgggatct ggctgccttc
     3001 cagcgccggg atcagcccgg cgtagtactc cagcacgtcc gcaccggtaa cgatatcgac
     3061 ggttgaggtt tccgaatatg cttttccggt gtcgagggtt tccagttttg cgagttcgtc
     3121 attgcgttca cgcagaatat caacggcccg acgcagaata cgcgagcgct ccatggcggt
     3181 catcgacgcc cagatttttt gcccctgcag ggcgcttttc acggcgcgat cgacatcctc
     3241 gcgcccggcg gcctgcacgg tcgccagcac gttaccgttg gccgggttaa tggtctcgaa
     3301 ggtgcgaccg ctggtggcgg aggtataacc accatgtata taaagctgct gttctgccat
     3361 tcgggacatc acgtctcctc ggttaatcgg tgggtaaatg ctgagtaata aagtgacggg
     3421 tcagcgagtg tgcgagaggt ttatccagcg gtttgccgct cagtgccgcg cgcagccata
     3481 atccgtcaat cagcgcggca aggccgtagc ccgcttcctg tgcctgttcg cgcggcaatt
     3541 cgcggcgaaa ctcgctcacc agattcgaca gcaagcggcg gctgctgacc tgctgtaaac
     3601 gatagagcat cggctgatgc atactgctgg cccagaacgc cagccaggct ttcatcgccg
     3661 cactgctcac ctgagtttca tcgaagtttc cggcaacaat cgcctgtaat cgctgctctg
     3721 cactgccctg cggaagtgca tgtaatcgat tcaaaaccgc gtcacgtaac tgactggtga
     3781 tgtcgcgcat ggttgcttcc agcagaccat ttttgtccct gaaatagtgg ctgatgatcc
     3841 ccgtagaaac gcctgcacgg cgggcgatct gcgcgatcgt tgcatcgtgc atgcccactt
     3901 catttattgc ttccagtgtg gcgtcgatca gttgtctgcg ccggatcgac tgcatcccca
     3961 atttgggcat tttcgccact ccattcatca gcgttgttta tctattaaag cggttattga
     4021 ttggacgttc aatataaaat gtgtcttaat tgttacgaat ttgattttaa atggtaacaa
     4081 taacagtggg gatactggat gacagacctt tcacacagca gggaaaagga caaaatcaat
     4141 ccggtggtgt tttacacctc cgccggactg attttgttgt tttccctgac aacgatcctg
     4201 tttcgcgact tctcggccct gtggattggc cgcacgctgg actgggtgtc taaaaccttc
     4261 ggttggtact atctgctggc ggcaacgctc tatattgtct ttgtggtctg tatcgcttgt
     4321 tcgcgttttg gttcggtgaa gctcgggcca gaacaatcca aaccggaatt cagcctgctg
     4381 agttgggcgg cgatgctgtt tgctgccggg atcggtatcg acctgatgtt cttttccgta
     4441 gccgaaccgg taacgcagta tatgcagccg ccggaaggcg cgggacagac gattgaggcc
     4501 gcgcgtcagg cgatggtctg gacgctgttt cactacggct taaccggctg gtcgatgtat
     4561 gcgctgatgg gcatggcgct cggatacttt agctatcgtt ataatttgcc gctcaccatc
     4621 cgctcggcgc tgtacccgat cttcggtaaa cggattaacg gacctatagg tcactcagtg
     4681 gatattgcag cggtgatcgg cactatcttc ggtattgcca ctacgctcgg tatcggtgtg
     4741 gtgcagctta actatggctt gagcgtactg tttgatattc ccgattcgat ggcggcaaaa
     4801 gcggcactga tcgccttgtc ggtgataatc gccacgatct ctgtcacctc cggtgtcgat
     4861 aagggcattc gcgtgttatc ggagcttaat gtcgcgctgg cgctgggatt gatcctgttc
     4921 gtattgttta tgggcgacac ttcgttcctg cttaatgcgc tggtgctgaa tgttggcgac
     4981 tatgtgaatc gctttatggg catgacgctc aacagttttg ccttcgaccg tccagttgag
     5041 tggatgaata actggacgct cttcttctgg gcatggtggg tggcatggtc gccgtttgtc
     5101 ggcttgttcc ttgcgcgtat ctcgcgtggg cgtaccattc gccagttcgt gctgggcacg
     5161 ttgattattc cgtttacctt cacgctgtta tggctctcgg tgttcggcaa tagcgcgctg
     5221 tatgaaatta tccacggcgg cgcggcattt gccgaggaag cgatggtcca tccggagcgc
     5281 ggcttctaca gcctgctggc gcagtatccg gcgtttacct ttagcgcctc cgtcgccacc
     5341 attactggcc tgctgtttta tgtgacctcg gcggactccg gtgcgctggt gctggggaat
     5401 ttcacctcgc agcttaaaga tatcaacagc gacgcccccg gctggctgcg cgtcttctgg
     5461 tcggtggcga ttggtctgct gacgctcggc atgctgatga ccaacgggat atccgcgctg
     5521 caaaacacca cggtaatcat ggggctgccg ttcagctttg tgattttctt cgtgatggcg
     5581 gggttgtata aatctctgaa ggtagaagat taccgccgtg aaagtgccaa ccgcgatacc
     5641 gcgccgcgac cgctggggct tcaggatcgc ctgagctgga aaaaacgtct ctcgcgcctg
     5701 atgaattatc cgggcacgcg ttacactaaa cagatgatgg agacggtctg ttacccggca
     5761 atggaagaag tggcgcagga gctgcggttg cgcggcgcgt acgtggagct aaaaagcctg
     5821 ccgccggaag agggacaaca gttggggcat ctggatttgt tggtgcatat gggcgaagag
     5881 caaaactttg tctatcagat ttggccgcag caatattcgg tgccgggctt tacctaccgc
     5941 gcacgcagcg gtaaatcgac ctactaccgg ctggaaacct tcctgttaga aggcagccag
     6001 ggcaacgacc tgatggacta cagcaaagag caggtgatca ccgatattct tgaccagtac
     6061 gagcggcacc ttaactttat tcatctccat cgtgaagcgc cgggccatag cgtgatgttc
     6121 ccggacgcgt gattgttatt gcataaaacc gcgccatgtc tgcatatggc gctgtttctc
     6181 ctacctcttg atacgttata tctatacggt taagccctta gtatctattg atgattacca
     6241 gacaacagat aataaaaaag aaaagaacta ttgca
//
LOCUS       DAPHJE010000191         6260 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPHJE010000191 DAPHJE010000000
VERSION     DAPHJE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.193"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..976
                     /gene="pfkB"
                     /locus_tag="RH222_004215"
     CDS             47..976
                     /gene="pfkB"
                     /locus_tag="RH222_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV3799138.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1077..1367
                     /gene="ghoS"
                     /locus_tag="RH222_004216"
     CDS             1077..1367
                     /gene="ghoS"
                     /locus_tag="RH222_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV3799139.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1473..2333
                     /locus_tag="RH222_004217"
     CDS             1473..2333
                     /locus_tag="RH222_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV3799140.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2374..2910)
                     /locus_tag="RH222_004218"
     CDS             complement(2374..2910)
                     /locus_tag="RH222_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV3799141.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3057..3725
                     /gene="hxpB"
                     /locus_tag="RH222_004219"
     CDS             3057..3725
                     /gene="hxpB"
                     /locus_tag="RH222_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV3799142.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3888..4478
                     /locus_tag="RH222_004220"
     CDS             3888..4478
                     /locus_tag="RH222_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3799143.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4611..6002
                     /gene="tcyP"
                     /locus_tag="RH222_004221"
     CDS             4611..6002
                     /gene="tcyP"
                     /locus_tag="RH222_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV3799144.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6006..>6260)
                     /locus_tag="RH222_004222"
     CDS             complement(6006..>6260)
                     /locus_tag="RH222_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799145.1"
                     /translation="NLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECI
                     DKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1538 a         1548 c         1571 g         1603 t
ORIGIN      
        1 tccgctgatt cggtgccaga ctgaaattag cctataggag gaaatgatgg tacgtatcta
       61 tacgttgaca cttgcgccct ctctcgatag cgcaacaatt accccgcaaa tttatcccga
      121 aggaaaactg cgctgtaccg caccggtgtt cgaacccggg ggcggcggca tcaacgtcgc
      181 ccgcgccatt gcccatcttg gaggcactgc cacagcgatc ttcccggcgg gtggcgcgac
      241 cggcgaacat ctggtttcac tgttggcgga tgaaaatgtc cccgtcgcta ctgtagaagc
      301 caaagactgg acccggcaga atttacatgt acatgtggaa gcaagcggtg agcagtatcg
      361 ttttgttatg ccaggcgcgg cattaaatga agatgagttt cgccagcttg aagagcaagt
      421 tctggaaatt gaatccgggg ccatcctggt cataagcgga agcctgccgc caggtgtgaa
      481 gctggaaaaa ttaacccaac tgatttccgc tgcgcaaaaa caagggatcc gctgcatcgt
      541 cgacagttct ggcgaagcgt taagtgcagc actggcgatt ggtaacatcg agttggttaa
      601 gcctaaccaa aaagaactca gtgcgctggt gaatcgcgaa ctcacccagc cagacgatgt
      661 ccgcaaagcc gcgcaggaaa tcgttaatag cggcaaggcc aaacgggttg tcgtttccct
      721 gggtccacaa ggagcgctgg gtgttgatag tgaaaactgc attcaggtgg tgccaccacc
      781 ggtgaaaagc cagagtaccg ttggcgctgg tgacagcatg gtcggcgcga tgacactgaa
      841 actggcagaa aatgcctctc ttgaagagat ggttcgtttt ggcgtagctg cggggagtgc
      901 agccacactc aatcagggaa cacgtctgtg ctcccatgac gatacgcaaa aaatttacgc
      961 ttacctttcc cgctaacaaa aacattcccc cagcattggg ggaatcatca ccaacctgtc
     1021 ggcaacgcgt ttctccgact atgctcaaaa gtcatgtgat aacaaagggg tgaactatgg
     1081 ccagtggcga tcttgtccgt tatgtcatta ccgtcatgtt gcatgaggat acattgactg
     1141 aaattaacga gttgaataat tacctgactc gcgacggttt tttgctcacc atgacggatg
     1201 atgagggaaa tatccatgag ctggggacta acacttttgg acttatcagt acccaaagtg
     1261 aagaagaaat tagagaactg gtttcggggc ttacccaaag tgcaaccggc aaagatcctg
     1321 aaatcaccat cacgacctgg gaggaatgga atagcaacag aaaataaatg gtttttgggc
     1381 aataatcagt ctgtggtgtg cgttagctcg tgtttttaca ccgcattctt gcgctaacct
     1441 tatgatctgg cagacaacat gggagagaca tcatgtggca ggcaatcagt cgtcttttga
     1501 gcgagcagtt aggtgaaggc gaaatcgaac tgcgtaatga actgcctggc ggagaagtcc
     1561 atgccgcatg gcatttgcgc tatgcaggac atgacttttt cgtcaaatgt gatgaaaggg
     1621 aactgcttcc cggttttacc gccgaagccg accaactgga gttactgtcg cgtagtaaaa
     1681 ccgtcaccgt gcctaaggtt tgggcagtag gcgctgaccg tgactacagt tttctggtga
     1741 tggattatct cccacctcgt ccgctggatg cgcatagcgc atttattctt ggtcagcaaa
     1801 tagcgcgttt acatcaatgg agcgaccaac cacaatttgg cctcgatttc gataacgcac
     1861 tctccacaac tccacagccc aacacctggc aacgtcgctg gtcaacgttt tttgctgaac
     1921 aacggattgg ctggcagctg gaactggcag cagaaaaagg gatcgctttc ggcaatatcg
     1981 acgccatcgt agagcatatt cagcagcgtc tcgcctcaca tcaaccgcag ccttctctgt
     2041 tgcacggcga tttatggtcc ggcaactgtg cgctgggtcc ggatggcccg tacattttcg
     2101 acccggcctg ctactggggt gaccgagagt gcgacctggc gatgttaccg ctgcatacag
     2161 aacagccgcc acaaatctat gacggctatc agtcagtatc cccgctacct gccgatttcc
     2221 ttgaacgtca accggtttac caactctaca cgctgttaaa tcgtgcaagg ttatttggcg
     2281 gtcagcattt ggttattgct cagcagtcac tggatagatt attagcagca tgatatgggt
     2341 tgaagataat ggccgctccg tgcggccttt tgattaaata aacccaagca gagaaaagaa
     2401 tacataacca gcagcaatac aaataaccgg caggatatac agtgagaaaa actgcaggaa
     2461 aatagtatgg tgcggaacaa caatacgtga ttcaatttgt tcacgcgtta gcccctcttc
     2521 ccctttggct ttttccagaa tgagttgatc ttcaacacct tctcgcaaga agcgcgcctg
     2581 gcgactcatc cgggcaccgg aatcctgcaa cgccagtccg acaaaaatca ggatgaaaat
     2641 cacccagaac ataacgttca cgccaccatt aaaattgggc gtcggggagt tataccagaa
     2701 cagattcaaa aaaggcgtat tcgcctgcat catatcgatc atgacatgag taaagtcgag
     2761 catgaccgca ttaatgcctt cctggttttc ctgccgggta ttcataaatt tcagcaaaga
     2821 aatcaatgta gagatcaatg caggtataaa aatcacccaa cccaaaatcc ttttcaaaac
     2881 agcaatgcgt ccagcttgtt gatacgtcat gagttctcct tgattaagac gcgtcgtttc
     2941 acttagttta cctgtagata tctgttttcg cccattcttt aaaggcgata tgataggcgc
     3001 ttaatcataa gcacggctta ataccttaca cataatgctc taaaggagag gttgtaatgt
     3061 caaccccgcg tcagattctt gctgcaattt ttgatatgga tggattactt atcgactcag
     3121 aacctttatg ggatcgagcc gaactggatg tgatggcaag cctgggggtg gatatctccc
     3181 gtcgtaacga gctgccggac accttaggtt tacgcatcga tatggtggtc gatctttggt
     3241 acgcccggca accgtggaat gggccaagcc gtcaggaagt agtagaacgg gttattgccc
     3301 gtgccatttc actggttgaa gagacacgtc cattattacc aggcgtgcgc gaagccgttg
     3361 cgttatgcaa agaacaaggt ttattggtgg gactggcctc cgcgtcacca ctacatatgc
     3421 tggaaaaagt gttgaccatg tttgacttac gcgacagttt cgatgccctc gcctcggctg
     3481 aaaaactgcc ttacagcaag ccgcatccgc aagtatatct cgactgcgca gcaaaactgg
     3541 gcgttgaccc actgacctgc gtagcgctgg aagattcagt aaatggcatg atcgcctcta
     3601 aagcagcccg catgcgttcc attgtcgttc ctgcgccaga agcgcaaaat gatccacgtt
     3661 ttgtattagc aaacgtcaaa ctctcatcgc tgacagaact caccgcaaaa gaccttctcg
     3721 gttaatgacc aggggcagtg atctcgctgc ccctggttct ttatctgaat ttgcattcaa
     3781 ctgacggatt aatcgtcaat ttaagagaaa gagttacacc gtcaccactt ccgtgcactg
     3841 tataaaaatc ctatactgta cgtatcgaca gtttagtgag ttttatcatg acggcggaag
     3901 gtcaccttct cttttctatt gcttgtgcgg tatttgccaa aaatgccgag ctgacgcccg
     3961 tgctggcaca gggtgactgg tggcatattg tcccttccgc aatcctgacg tgtttgttac
     4021 cggacatcga tcacccaaag tcgtttcttg ggcagcgatt aaagtggata tcaaaaccga
     4081 tcgcccgcgc ttttgggcat cgtggtttta cccacagtct gctggcggta tttgcgctgc
     4141 tggcaacctt ttaccttaag gttcctgaaa gctggttcat tccggctgat gcgctacaag
     4201 gaatggtgct gggttattta agccacatac ttgccgatat gctgacaccc gccggtgttc
     4261 ccctgctctg gccatgtcgc tggcgtttcc gcttgcctat cctggttccc caaaagggca
     4321 accaactgga acgttttatc tgcatggcat tatttgtctg gtcggtatgg atgccccatt
     4381 cattacccga aaacagcgcg gttcgttggt catcgcaaat gatcaatacc ttgcagatcc
     4441 agtttcatcg gcttattaag catcaggttg aatactaaaa aggcaaaaat cacctttctg
     4501 gaataagcaa ttccatttga atataagagt cagctcacag ttctgttaat cttgcgccaa
     4561 cactatgact gctacgcagt gatagaaata ataagatcag gagaacgggg atgaactttc
     4621 cattaattgc gaacatcgtg gtgttcgttg tactgctgtt tgcgctggct cagacccgcc
     4681 acaaacagtg gagtctggcg aaaaaagtgc tggtgggtct ggtgatgggt gtggtttttg
     4741 gccttgccct gcataccatt tatggttctg acagccaggt acttaaagat tctgtacagt
     4801 ggtttaacat cgttggtaac ggctatgttc aactgctgca aatgatcgtt atgccgttag
     4861 tcttcgcctc tattctgagc gcggttgccc gtctgcataa cgcatctcag ttaggcaaaa
     4921 tcagttttct gaccatcggt acgcttttgt ttaccacact gattgcggcg ctggtcggtg
     4981 tgctggtcac caacctgttt ggtttgacgg ctgaaggtct ggttcagggt ggtgcagaaa
     5041 ctgcacgtct gaacgccatc gaaagtaact atgttggtaa agtctctgac ctgagcgttc
     5101 cgcagctggt cttgtccttt atcccgaaaa acccgtttgc cgatctgacc ggagccaatc
     5161 cgacgtcaat tatcagcgtg gtaatttttg ccgcattcct cggtgtagct gcgctgaaac
     5221 tgctgaagga tgatgcgccg aaaggtgaac gcgtcttaac cgctatcgat accctgcaaa
     5281 gctgggtgat gaaactggtt cgcctggtca tgcagttgac cccttacggc gttctggcac
     5341 taatgaccaa agtggttgca ggttctaacc tgcaagacat catcaaactg ggaagtttcg
     5401 ttgtcgcgtc ctacctcggt ctgctgatta tgtttgcagt gcatggcatt ctgctgggca
     5461 ttaatggcgt gagtccgctg aagtacttcc gtaaggtatg gcctgtgctg acgtttgcct
     5521 ttaccagccg ttccagtgct gcgtctatcc cactgaatgt ggaagcacaa acgcgtcgtc
     5581 tgggcgttcc tgaatccatc gccagtttcg ccgcctcttt cggtgcgacc attggtcaga
     5641 acggctgcgc cggtttgtat ccggcaatgc tggcggtgat ggttgcgcct acggttggca
     5701 ttaacccgct ggacccgatg tggattgcga cgctggtcgg tattgttacc gttagttccg
     5761 caggcgttgc cggtgtcggt ggtggtgcaa ctttcgccgc actgattgtg ctgcctgcga
     5821 tgggcctgcc agtaaccctg gtggcgctgt taatctccgt tgaaccgctt atcgacatgg
     5881 gccgtacggc gctaaacgtt agtggctcga tgacagctgg cacgctgacc agccagtggc
     5941 tgaagcaaac cgataaagcc attctggata gcgaagacga cgccgaactg gcacaccgtt
     6001 aattcttatg ctggcaacgg tccgttttgt ataggggccg ttgctttact ttttaaatgt
     6061 tccatcgcgg cgatgcatac tgactcatat ttaaacagat cgtcagcgaa aaaaatgtcc
     6121 aacttatcaa tacattcctg gctcaatgaa tgtgcctgta gtgagaagct taaaacctca
     6181 tacccgagac gttttctaag tgaactatct ggaatactca taatatcttc aagcacacca
     6241 cttcccattc ccggaagatt
//
LOCUS       DAPHJE010000192         6141 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPHJE010000192 DAPHJE010000000
VERSION     DAPHJE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.194"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            80..856
                     /gene="exuR"
                     /locus_tag="RH222_004223"
     CDS             80..856
                     /gene="exuR"
                     /locus_tag="RH222_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV3799146.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1201..1863
                     /locus_tag="RH222_004224"
     CDS             1201..1863
                     /locus_tag="RH222_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3799147.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1867..2250
                     /gene="mzrA"
                     /locus_tag="RH222_004225"
     CDS             1867..2250
                     /gene="mzrA"
                     /locus_tag="RH222_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV3799148.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2397..2765
                     /locus_tag="RH222_004226"
     CDS             2397..2765
                     /locus_tag="RH222_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV3799149.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2803..3108
                     /locus_tag="RH222_004227"
     CDS             2803..3108
                     /locus_tag="RH222_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV3799150.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3111..3515
                     /locus_tag="RH222_004228"
     CDS             3111..3515
                     /locus_tag="RH222_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV3799151.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3505..3804
                     /locus_tag="RH222_004229"
     CDS             3505..3804
                     /locus_tag="RH222_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV3799152.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3990..4382
                     /locus_tag="RH222_004230"
     CDS             3990..4382
                     /locus_tag="RH222_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV3799153.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4452..5438
                     /gene="yqjG"
                     /locus_tag="RH222_004231"
     CDS             4452..5438
                     /gene="yqjG"
                     /locus_tag="RH222_004231"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV3799154.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQNEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DTLGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5731..6096
                     /locus_tag="RH222_004232"
     CDS             5731..6096
                     /locus_tag="RH222_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3799155.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1539 a         1509 c         1549 g         1544 t
ORIGIN      
        1 aatttatata tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat
       61 tccactccgg gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc
      121 cgctgacctg aaagagcgca tcgaacaggg cgtctatctg gtaggtgata aactgcctgc
      181 agaacgcttt attgccgatg aaaagaacgt cagccgtacg gtagttcgtg aagccatcat
      241 catgctggaa gttgaaggct atgtcgaagt gcgtaaaggt tcaggtattc atgtggtttc
      301 caaccagccg cgccatcagc aggcagctga caataatatg gaattcgcca attacggtcc
      361 gtttgagttg cttcaggctc gccagctcat cgaaagtaat attgccgaat tcgcggcaac
      421 tcaggtaacg aaacaggaca tcatgaaact gatggccatt caggaacagg cgcgcggcga
      481 acaatgcttc cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac
      541 gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg acacaacgta gccataaccc
      601 gtactggaaa aaactgcacg aacacattga ttcccgtacc gtcgataact ggtgtgatga
      661 ccacgatcaa atcctcaagg cgctgattcg caaagatcct catgctgcta agctggcaat
      721 gtggcagcac ctggaaaaca ccaagatcat gttattcaac gaaaccagcg acgacttcga
      781 gttcaatgcc gaccgctatc tattcgccga aaacccggtg gtgcatctcg atacggccac
      841 cagtggcagt aaatgaaata cctgtccgac aggcgctgcg acgcgcctgt ttgtctcgtg
      901 ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat
      961 tcagcaaaat cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt
     1021 acaattagat tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag
     1081 ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac
     1141 attgcggtag tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga
     1201 atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc
     1261 aatccatcga tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac
     1321 ggcttgcttc cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg
     1381 attgcgaaag gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc
     1441 ctcggctgct gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa
     1501 aactggctat ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa
     1561 cacggtttat cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg
     1621 acgattgccg ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc
     1681 ggtctgctgt gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccggta
     1741 tttttaaagt acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg
     1801 gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg
     1861 taagggatgc aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta
     1921 cttctggtcg gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg
     1981 gcgattcgtg ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac
     2041 cttgacgctc atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt
     2101 acatttgact ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc
     2161 catggctaca tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg
     2221 ttacgggata actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact
     2281 ttggtgattt caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg
     2341 ttgtctggac aatcgttcct ttgtaatagg tccgaatcac aatggaaggt tcaagaatga
     2401 aataccgcat cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta
     2461 ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc
     2521 aaaaccagaa tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt
     2581 cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg
     2641 cggaacgcca gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca
     2701 aacgcgaacg gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact
     2761 actaactcac aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact
     2821 acggaacatc tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc
     2881 tcatctggcg agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca
     2941 ctgaaacaga gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt
     3001 gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggaatt
     3061 ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca
     3121 ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca
     3181 tcatggttga aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa
     3241 aagctaatct ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg
     3301 gtcttatgag cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg
     3361 cgatgattgc caccaccgtg gtgttgctgc tactggcact aattggcggt atctggacgt
     3421 tacgtaaatc gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc
     3481 ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg
     3541 caactgctta gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg
     3601 ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg
     3661 gcgctggttg gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc
     3721 cgctgggcca gacgcggttt tggcgtatgg agcgcctggc gtctggttaa aacgaccctc
     3781 aagcagcaac agcttcgcgg ttaacttccc tctggccgga gcaattccgg ccttatccat
     3841 caaatttttt gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta
     3901 tgattctctc catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca
     3961 gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg
     4021 tagcgcgcat tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt
     4081 actctggtac ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg
     4141 tgattctgct tgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca
     4201 cagccctgtt tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaactttg
     4261 ctgaaggcgt caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc
     4321 tgctggcaat taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt
     4381 aagcacgcgc tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg
     4441 aggagagaaa aatgggtcaa ctgattgacg gcgtctggca tgacacctgg tacgatacca
     4501 aatctaccgg cggtaaattt caacgttcag cttccgcatt tcgtaactgg ctcactgccg
     4561 atggcgcacc tggccccact ggcacaggcg gttttatcgc agagaaagat cgttatcatc
     4621 tctatgtttc actcgcctgc ccgtgggcgc accgcacgct gatcatgcgc aaactcaaag
     4681 gactggaacc gtttatttcc gtttccgtag tgaacccgct gatgctggaa aacggctgga
     4741 cctttgatga cagttttccg ggagcaaccg gagatacgct ctatcaaaat gaatttctgt
     4801 atcagcttta tctccacgcc gatccacact acagcggacg agttactgtt cccgtgctgt
     4861 gggacaaaaa gaaccacacc atcgtcagca acgaatcagc agaaatcatc cgcatgttca
     4921 ataccgcgtt tgatacgctg ggcgcgaaag cgggtgatta ctacccacca gccctgcaaa
     4981 cgaaaattga cgaacttaac ggctggattt atgacaccgt taacaacggc gtgtataaag
     5041 ctggttttgc caccagccag gaagcttacg acgaggcggt ggcgaaagtg tttgaatcgc
     5101 tggcgcgact ggagcagatt ttaggccagc atcgttacct gaccggcaat cagctaaccg
     5161 aagccgatat tcgcctgtgg accacgctgg tgcgttttga tccggtgtat gtgacccact
     5221 tcaagtgtga taagcaccgc atcagcgatt accttaatct gtatggcttc ctgcgtgata
     5281 tctaccagat gccgggaatt gccgaaacag tcaatttcga tcacatccgt aatcattact
     5341 tccgcagcca taagaccatc aaccctacgg ggattatttc aattggtcca tggcaggatc
     5401 tcgatgaacc gcatggacga gatgttcgct tcggttaaaa aaaggcacct tacgaggtgc
     5461 ctctgataat tagctaagtg catgttgttc atgtcggatg cggcgtgaac gccttatcaa
     5521 acctacaaaa catttatatt caatatgttg caatgaccgc gcaggccaga taagcgtagc
     5581 gcatctggcg attttgcgct tgtcatcagt cttagcgcgc cttcacagca ctttattaat
     5641 tccttgaacg aatatttact gccatttcat tcacgtctat tcttaatttg ctgctttaaa
     5701 aacaagtgat tgagcaaaat tgaggcaaaa atggactggt atctgaaagt actaaaaaat
     5761 tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac
     5821 atcatcttta cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt
     5881 ggcgaaggca tcctgacgac aatctacggt attctggtgt ttttaccgtg gtgggcagtt
     5941 cagttccgcc gcctgcacga caccgaccgc tcggcgtggt gggcactgtt gttcttaatc
     6001 ccgtttatcg gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa
     6061 aaccgctttg gaccagatcc gaaactggaa ccttaaaaca aatctgctcg ccggatgtct
     6121 gactcatccg gcttttatca t
//
LOCUS       DAPHJE010000193         6060 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPHJE010000193 DAPHJE010000000
VERSION     DAPHJE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.195"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            217..1320
                     /gene="ompC"
                     /locus_tag="RH222_004233"
     CDS             217..1320
                     /gene="ompC"
                     /locus_tag="RH222_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3799156.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1432..2487
                     /gene="apbE"
                     /locus_tag="RH222_004234"
     CDS             1432..2487
                     /gene="apbE"
                     /locus_tag="RH222_004234"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV3799157.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2561..3625
                     /gene="ada"
                     /locus_tag="RH222_004235"
     CDS             2561..3625
                     /gene="ada"
                     /locus_tag="RH222_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV3799158.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3625..4275
                     /gene="alkB"
                     /locus_tag="RH222_004236"
     CDS             3625..4275
                     /gene="alkB"
                     /locus_tag="RH222_004236"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV3799159.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4351..5994
                     /gene="yojI"
                     /locus_tag="RH222_004237"
     CDS             4351..5994
                     /gene="yojI"
                     /locus_tag="RH222_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV3799160.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1447 a         1527 c         1637 g         1449 t
ORIGIN      
        1 tcgccattcc gcaataatct taaaaagttc ccttgcattt acattttgaa acatctatag
       61 cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt
      121 ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa
      181 aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc
      241 ctggtcccag ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac
      301 ggcaacaaat tagatctgta cggtaaagta gacggcctgc actatttctc tgacaacaaa
      361 gatgtagatg gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact
      421 gaccagctga ccggttacgg ccagtgggaa tatcagattc agggcaacag cgctgaaaac
      481 gaaaacaact cctggacccg tgtggcattc gcaggtctga aattccagga tgtaggttct
      541 ttcgactacg gtcgtaacta cggcgttgtt tacgacgtaa cttcctggac cgacgtactg
      601 ccagaattcg gtggcgacac ctacggttct gacaacttca tgcagcagcg tggtaacggc
      661 ttcgcgacct accgtaacac tgacttcttc ggtctggttg acggcctgaa ctttgctgtt
      721 cagtaccagg gtaaaaacgg tagcgtaagc ggcgaaggca tgaccaacaa cggtcgtggt
      781 gctctgcgtc agaacggcga cggcgttggc ggatctatca cttatgatta cgaaggcttc
      841 ggtatcggtg gtgcgatctc cagctccaaa cgtactgatg atcagaacag cccgctgtac
      901 atcggtaacg gcgaccgtgc tgaaacctac accggtggtc tgaaatacga cgctaacaac
      961 atctacctgg ctgctcagta cacccagacc tataacgcaa ctcgcgtagg ttccctgggt
     1021 tgggcgaaca aagcacagaa ctttgaagct gttgctcagt accagttcga cttcggtctg
     1081 cgtccgtctg tagcatacct gcagtctaaa ggtaaaaacc tgggtaccat cgctggtcgt
     1141 aactacgacg acgaagatat cctgaaatat gttgatgttg gtgctaccta ctacttcaac
     1201 aaaaacatgt ccacctacgt tgactacaaa atcaacctgc tggacgacaa ccagttcact
     1261 cgtgacgctg gcatcaacac tgataacatc gtagctctgg gtctggttta ccagttctaa
     1321 tctcgattga tatcgaacaa agggcctgcg ggcccttttt tcattgtttt cagcgtacaa
     1381 acacagtttt ttggtgtact cttgcgaccg ttcgcatgag gataatcacg tatggaaata
     1441 agctttaccc gcgtggcact gctggctgcc gcgctctttt ttgttggttg cgatcaaaaa
     1501 ccacaacccg ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt
     1561 accttctggc gtgccagcat cccgggcatt gatgccaaac gcagtgccga acttaaagaa
     1621 aagattcaga cccagctgga cgccgacgat cagctgcttt cgacctataa aaaagattcc
     1681 gcgctgatgc gctttaacga ttcgcaaagt ttatcgccgt ggccggtaag tgaagcgatg
     1741 gccgatatcg tcaccacctc gctgcgcatt ggcgcgaaga ccgatggcgc gatggatata
     1801 accgtcgggc cgctggtgaa tctgtggggc tttggcccgg aacaacagcc ggttcaaatt
     1861 ccgagccagg aacagatcga tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc
     1921 attaatcagt cgcatcagca atatctgcaa aaagacctgc cggatttaaa tgtcgatctc
     1981 tctaccgtcg gcgaaggtta tgcggcggat cacttggcac gcttgatgga gcaggaaggg
     2041 atttcccgct atctggtgtc ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa
     2101 ggccagccgt ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc ggttcaggct
     2161 gtggtggata tcaacggtca tgggattagc acctctggca gctaccgtaa ctattacgaa
     2221 ctggacggca aacgtctttc ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac
     2281 aatctggtat ccgtgacggt gattgctccg acggcgctgg aagccgatgc ctgggatact
     2341 ggcttgatgg tactcgggcc ggagaaagcc aaagaggttg ttcgccggga agggctggcg
     2401 gtttatatga tcaccaaaga aggcgatagc tttaaaacct ggatgtcacc acagtttaaa
     2461 agcttccttg tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc gtgatggtga
     2521 ccgggcagac taaaggctat ccttaaccag ggagctgatt atgaaaaaag ccacatgctt
     2581 aactgacgat caacgctggc aatctgtctt agcccgcgac ccgaatgccg acggcgaatt
     2641 cgttttcgcc gtgcgtacca caggcatctt ttgccgtccg tcttgccgcg ccagacatgc
     2701 tttgcgggaa aacgtctcct tctacgcaaa tgccagcgag gcactcgccg ctggctttcg
     2761 cccctgcaaa cgttgtcagc cagaaaaagc caatgcccag caacatcggt tggataaaat
     2821 cacccacgcg tgtcgactgc tggaacagga aacgcctgta acgttggaag ccttagccga
     2881 ccaggtggcg atgagtccat ttcatctaca tcggttgttt aaagcgacta ccggaatgac
     2941 gcctaaagcc tggcaacagg cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg
     3001 ggagagcgtg acgacgtcta ttcttaacgc cggattcccc gacagcagca gttactatcg
     3061 caaagctgac gaaacgctgg gcatgacggc taaacaattc cgtcacggtg gcgaaaatct
     3121 ggcggtgcgt tacgcgctgg ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga
     3181 gcgggggatt tgcgcgatat tgctgggcga tgatgacgcg acactaatca gcgagttgca
     3241 gcagatgttt cccgctgccg acaacgcgcc tgccgatctg atgtttcagc aacatgtgcg
     3301 tgaagtgatc gccagcctca atcaacgcga tacgccgctg acgttaccgc tggacattcg
     3361 cggcactgct tttcagcaac aagtctggca ggcactgcgc acgatacctt gcggtgaaac
     3421 cgtcagttat cagcaactgg ctaacgccat cggcaaaccg aaagcggtac gggccgttgc
     3481 cagcgcctgt gccgccaaca agctggctat cataataccc tgtcatcggg tggtccgtgg
     3541 tgatggcaca ctttccggtt accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg
     3601 cgaagctgaa aatgaggaga ggtaatgttg gatctgtttg ccgatgctga accgtggcaa
     3661 gagccactgg cggctggagc ggtaattttg caccgttttg cttttaacgc tgcggagcaa
     3721 ctaatccgcg atattaatga cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc
     3781 gggggatata ccatgtcggt ggcgatgacc aactgtgggc atctgggctg gacgagccat
     3841 cggcaaggtt atctctattc gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg
     3901 ccacagagtt ttcataattt atgtcaacgt gcggctacgg cggcgggcta tccagatttc
     3961 cagccagatg cttgtcttat caaccgctac gctcctggcg cgaaactgtc gctgcatcag
     4021 gataaagacg aaccggatct gcgcgcgcca attgtttctg tttctctggg cttacccgcg
     4081 atttttcaat ttggtggcct gaaacgtaat gatccgctca aacgtttgtt gttggaacat
     4141 ggcgatgtgg tggtatgggg cggtgaatcg cggctgtttt atcacggtat tcaaccgttg
     4201 aaagcggggt ttcatccact caccaccgac tgccgctaca acctgacatt ccgtcaggca
     4261 ggtaaaaaag aataaaaata agaattatta ttgctgtgcg cgcgaagatg tttaaactgc
     4321 gggctgtaat tcattgtccg ggttttctgc atggaacttc ttgtacttgt ctggcggcag
     4381 tatcgctggc catttatcag tgtgatggcg ctaagcctcg ccagtgcggc attaggcatt
     4441 ggcttaattg cttttatcaa tcagcgcctt atcgaaacgg cggataccag tctgctggtg
     4501 ttgccggagt ttcttggatt attactgctg ttgatggcag tcactctcgg atcgcaactg
     4561 gcgctcacca ctttggggca tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg
     4621 attctggata cccacgtcga gcgcattgaa caactcggta gcgcctcgtt gctggcggga
     4681 ttaaccagcg atgtgcgcaa tatcaccatt gcttttgtgc gtcttccgga actggtgcag
     4741 gggatcattc tcactatcgg ttccgcggcg tatctgtgga tgctgtcggg caaaatgttg
     4801 ctggtaacgg ctatctggat ggcgatcacc atctggggtg gttttgtact ggtggcgcgg
     4861 gtgtacaaac atatggcgac cctgcgtgaa accgaagaca agctgtacac ggattttcaa
     4921 accgtactcg aagggcgcaa agagctgact ctgaaccggg aacgcgccga gtatgtgttt
     4981 aacaacctct acatccccga tgcgcaagag tatcgccatc atattattcg cgcagacacc
     5041 ttccatctta gcgccgtgaa ctggtcaaac atcatgatgc tgggcgctat cggcctggtg
     5101 ttctggatgg cgaacagtct cggttgggct gataccaacg ttgctgcgac ctattcgttg
     5161 acgctgttat tcctgcgtac gccgctgctt tcggcggttg gcgcattgcc gacgctgctg
     5221 acggcgcagg tggcgtttaa caagctgaac aaattcgcgc tcgcgccttt caaagctgag
     5281 tttccgcgcc cgcaggcttt tcccaactgg caaacgctgg agctgcgtaa cgtgacgttt
     5341 agctatcagg ataacgcgtt ttccgttggg ccgattaacc tcaccattaa acgtggcgag
     5401 ctgctgtttc tgattggtgg caacggtagt ggaaaatcga cgctggcgat gttgctgacg
     5461 ggcttgtacc agccacaaag cggcgaaatc ttgctggatg gcaaacctgt cagtggcgaa
     5521 caaccggaag attatcgcaa actgttttcg gcagtgttta ccgatgtctg gctgtttgat
     5581 caactgctgg ggccggaggg taaacccgct aacccgcaac tggttgagaa gtggctggcg
     5641 cagctgaaaa tggctcataa gcttgagtta agcaacgggc gtattgttaa cctgaagtta
     5701 tcaaaagggc agaaaaaacg cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt
     5761 atcctgctgg atgaatgggc ggcggatcag gatccacact tccgtcgtga gttttatcag
     5821 gtgttgctgc cgctgatgca ggagatgggt aaaactattt tcgctatcag tcatgatgat
     5881 cattacttta tccacgccga ccgcctgctg gaaatgcgca atgggcaact tagcgagctg
     5941 acgggcgaag agcgcgatgc cgcttcgcgt gatgccgttg cccggacggc ataatattac
     6001 gacgctgacc tggcggactg ctgccgtcag gtcaatatgc taaaaatccc ttcatttcta
//
LOCUS       DAPHJE010000194         5874 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPHJE010000194 DAPHJE010000000
VERSION     DAPHJE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.196"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(370..1329)
                     /gene="nrdF"
                     /locus_tag="RH222_004238"
     CDS             complement(370..1329)
                     /gene="nrdF"
                     /locus_tag="RH222_004238"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV3799161.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1339..3483)
                     /gene="nrdE"
                     /locus_tag="RH222_004239"
     CDS             complement(1339..3483)
                     /gene="nrdE"
                     /locus_tag="RH222_004239"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV3799162.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYKIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3456..3866)
                     /gene="nrdI"
                     /locus_tag="RH222_004240"
     CDS             complement(3456..3866)
                     /gene="nrdI"
                     /locus_tag="RH222_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV3799163.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3863..4108)
                     /gene="nrdH"
                     /locus_tag="RH222_004241"
     CDS             complement(3863..4108)
                     /gene="nrdH"
                     /locus_tag="RH222_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV3799164.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4356..4685)
                     /locus_tag="RH222_004242"
     CDS             complement(4356..4685)
                     /locus_tag="RH222_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV3799165.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4837..5181
                     /locus_tag="RH222_004243"
     CDS             4837..5181
                     /locus_tag="RH222_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV3799166.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5218..5667)
                     /gene="alaE"
                     /locus_tag="RH222_004244"
     CDS             complement(5218..5667)
                     /gene="alaE"
                     /locus_tag="RH222_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV3799167.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1509 a         1435 c         1447 g         1483 t
ORIGIN      
        1 taatttaatt gccatgcaat agaaagattc ctttatttgt ttatgtcgat atattaccca
       61 atggaaatag tcactttttt ctaccctaac atactgagaa tctgaggcaa cccctgaggg
      121 caaatgtggc atgaatattg atgcgagtaa atcgccggaa tttcccgtga tataagggct
      181 gagagcaaat cgagaaaagt tgatattttt tgtcaaagat agcgaaaaaa cgcctgaata
      241 ttgtggtttc aggcgaataa tgcaacagag tatcagtgta gatcaccaca aaatatttgc
      301 gtgataaaaa gctatttggc gggaattatt tccctgtcgc gggtagtgat atttttgaaa
      361 ataacaccct cagaaattcc agtcttcatc ttctgtttca accgctttcc ccatcacata
      421 agaggagcct gaaccggaaa agaaatcgtg attttcatcg gcattcggcg aaagcgcggc
      481 gaggattgcc ggattcactt ccgccatttc tgcgggaaat aaaggttcgt agcccagatt
      541 catcaaagcc ttattggcgt tgtaacagag aaacgctttc acatcgtcag cccacggggt
      601 ttcggcgtac agctcatcgg tgtattgcaa ctcgttgtcg tagagttcca gcaacaaatc
      661 gaaggcgaaa ctcttcaact cttcacgttg tcccagagat atcttttcca tgtttttctg
      721 atatttatag cctatgtagt aaccgtgtac tgcttcatcg cggataatca gacggatcag
      781 gtctgcggta ttggtcagct ttccgcggct ggaaaaatac atcggcagcc agaaaccgga
      841 atagaacaaa aaagattcaa gaaacacact ggcgattttc tttttcagcg gatcatcacc
      901 gcgataatgt tgctgaataa tctgagcttt tcgctgcaac ggtgcgtttt cttcactcca
      961 ggcgtaggcg gcatcgacat ctttggtctg gcacagcgtc gagaaaatcg aactgtaaga
     1021 gcgggcatga accgcttcca taaagctgat attcgataat accgcttctt catgaggcgt
     1081 gagtgcatcg ggcatcagcg aaggcgcgcc gataacattt tgcagcgtgt cgagcagcgt
     1141 caggccagta aaaacgcgca tcgtcagttg ttgttctacg acagttaatg tctgccaggc
     1201 aggaatatcg ttcgacagcg gcaccttttc tggtagccag aaattgctgg tcaggcgatt
     1261 ccacacctcc agatctttat cgtcagatat cttgttccag ttgatggcgc tgatacgtga
     1321 gagtttcata gatattcctt aaagtgcaca ggagacgcag ccttcaattt cagtgccttc
     1381 cagcgccatc tgacgcaggc ggatgtaata gagcgttttg atacctttgc gccaggcgta
     1441 tatctgcgct ttgttgatat cgcgagtggt ggcggtatcg gggaaaaaca gcgtcagcga
     1501 cagcccctga tcgacatggc gagtcgcttc cgcgtaggtg tcgatgatct tttctgcgcc
     1561 aattttgtaa gcgtcctgat acagcgccag attttcgtta gtcataaacg gggcagggta
     1621 gtaaacgcgt cctgttttgc cctctttgcg tatctctact ttcgccacaa tcggatgaat
     1681 actcgacgta gcatggttga tataagagat agaaccggtt ggcggcaccg cctggagatt
     1741 ctggttgtat atgccgtagc gcatcacgtc gtcgcgcagt tgcgcccaca tctcacgggt
     1801 aggtaacgta ataccgctgc gggcaaacag ttcgccaact ttcgccgttt tcggctgcca
     1861 gttaccttgc agatattggc tgaaatattc accactggca tagcgtgact gtttgaaccc
     1921 ggcgaaggtt tcaccgcgtt cgcgcgccag caacatcgag gtacgcagtg cgtgccaggt
     1981 gatggtatag aaatagagat tggtgaaatc cagggcttcc ggcgaaccat aagcgatgcc
     2041 ttccctcgcc agataaccat gtaaattcat ctgccccagt ccgatggcgt gcgaggcggc
     2101 atttccggct tcgatggacg gcacgctgcg gatatgactc atatctgaca ccgctgttaa
     2161 accacgtacg gcagtctcta ccgtgcgggc aaagtcgggg gaatccatgg tgtgagcaat
     2221 attcaacgaa cctaaattgc aggaaatatc atggcctgta cgggcatagt cgagattctc
     2281 gtcatactct gaggcgctgt taacctgcaa aatttctgag cagagattac tcatatttat
     2341 gcgcccggcg atagggttag cacggtttac cgtgtcttca tacatgatgt agggatagcc
     2401 ggactcaaac tggatttctg ccagtcgctg gaagaaatca cgggcgttga ggtatttttt
     2461 gcgaatgcgt tcatcggcaa ccagttcgtc atagtgttgg ctgatggcca catcggcaaa
     2521 cggcttgcca taaactcgct caacgtcata gggcgaaaac agcgccatct gcgcattctc
     2581 ttttgccaga tggaaagtga tatccgggat caccacgccc agcgacagtg ttttaatgcg
     2641 gattttctcg tcggcatttt cccgtttcgt atcgagaaaa cgcagaatat cgggatgatg
     2701 ggcatgtaaa tagactgcac cagccccctg acgagcgccg agctggttgg cataggaaaa
     2761 tgcgtcttcc agcattttca tcaccggaat cacgccagaa gattgatttt caatacgttt
     2821 aattggcgcg cccgcttccc gcagattcga cagcaaaaat gccacgccgc cgccgcgttt
     2881 cgacagttgc agtgcggaat ttaccgcccg gccaatcgac tccatattgt cttcaatacg
     2941 cagcaaaaaa caggaaacca gttcgccgcg ctgctgctta ccacagttga ggaatgtcgg
     3001 tgttgcaggc tgaaagcgtc ctgacagcat ctcatcggtc agttgcaacg ccagtgtttc
     3061 atcgccttgt gccagcgtta gcgccaccat cgtgactcga tcggcaaaat cttccagata
     3121 acgcttaccg tcgaatgtct tcaacgtata gctggtgtaa aacttccatg ccccgaggaa
     3181 tgtatggaaa cgaaaaccgc tggtgtgtgc gtgggcaaac agcgtaatga caaagtcgcg
     3241 agagtagcga ttaagaacgc tttcatcata gtaaccttcg ttgaccagcc agttcaggcg
     3301 ctgctgctgg ctactgaagg tcacactgtt cggacgcaca tgcgtcgcca taaaggcgtc
     3361 aacggcctgg cggtctttat cgaactgaat gcgacctgcg ctatcgtaga ggttaagcat
     3421 cgcattcagc gcgtggtaat ccatcgtttc ctgcgtcagg cattctgcgg ttgtcgttgc
     3481 caaaattcgg ttactccttt acgaacgttt tcgatatcgc tttgcgtacc catgagttca
     3541 aaacggtaca gccacggcac gccgcatttc tgagcaatca catctccggc acggccatac
     3601 gcctcaccaa agttgcgatt acccgaagca ataacgccgc gaagcaacgc ccggttgtgc
     3661 tcgtcgttta aaaagcgaat tacctgtcgt ggcaccgcgc cagccgtacc gccgccgccg
     3721 taagagggca cgatcaggat gtaaggctcg tctacctgaa tccgttcccg ctcattgagc
     3781 gggatgcgca ccgcgggcag acctaaacgt tcgataaaac gctgcgtatt ttcggagctg
     3841 ctggagaagt agacgagctg gctcatgcac tggccgcgtg tggcgctgga tgcagacggt
     3901 taatcatgtc cggacggaaa ccagaccagc taagatcgcc agcaatcact accggcaact
     3961 gacgaaagcc ctgagcacgc aacgcttccg ctgcttcagg aacgcgatcg acattaatca
     4021 tttcaaaatc aaagccccgg ttttccatcg cccgtttggt ggcgtggcac tgaacgcaat
     4081 cgttacgagt gtaaatagta atgcgcatga ttcgtatttc cgtttaaaat gaagatacgg
     4141 cgcgatgata cgcgtcgggt tgtctctctg ttgatacaga gatactagat gtagttgaaa
     4201 aaagattcaa ccacacaata tatagcaaat agtggtcgaa attaccctgg atatgagcgt
     4261 gacggggtag ggggattttt gtgattcacc aggcaaaaag aaaccccgaa gacaggcttc
     4321 ggggtcaaag acgcgtattt attatcattt ttgcactacg atttgcgcat gcttaacagt
     4381 gcgccgataa aaatacctac cgcagctgct gtacccacgc tacaccaggg tctttcacga
     4441 acaaaagaat ccgcgcagcc aacggcatcg cgcgcggctt gctggacgcg agtacgacca
     4501 tgcattcgcg ccctggtttc tttcagcaat gcctgagctt tactgcgtgc ggcttcagct
     4561 tcccctttgg cgtcgctgcc ccaggacttt aatacagatt cgaggctgtc agctaattga
     4621 ttgacatcat tctggatatc ctgcacgcca tcatcaacgt cgttgcggtt cggtcggtta
     4681 aacatatgat cccccattag aattcgatgt aattttcagt gtagaccata attttcttaa
     4741 ctgaggttca tcgcggcttt atggactttt ctgaaagcgt tgtgaatgcg caatcaggta
     4801 aggtaggccc gcggttgata ataacgagga tcaaatatgt atttacgacc agacgaggtg
     4861 gcgcgcgtac ttgaaaaagt cggttttact gtcgatgtgg taacccagaa agcgtatggt
     4921 taccgccgtg gggaaaatta tgtctatgtt aatcgcgaag cgcgaatggg acgtaccgct
     4981 ctggtaattc atccaacatt aaaagaacgc agttcaacac tggctgaacc cgcttccgat
     5041 attaaaactt gcgatcatta ccagcaattt ccgctctatt tagcaggcga gcgacacgag
     5101 cattacggta tcccgcatgg cttcagttcg cgtgttgcgc ttgaacgtta tttgaatggt
     5161 ttatttggcg aagccagtta aagacgcgac tggcgatgcc agtcgcgaaa agaagagtca
     5221 ggcttttacc tgctggtaac ggctgacttt aaacagtcgg cggcaataat cgaggaagta
     5281 gccataaacc gcccccatca acatcgaaac aacgatgttt gaactgaccg ccgccataat
     5341 ctgatgccag tctgcgccca ccactaacaa gatcgccaca tacaccggtg actggaacgt
     5401 cacataagcc aggatatcag ccagattttt tatccagccc gacgggctaa ctttgcgtgc
     5461 cgcgcgcata aacagatcgc ggtacatacc gtatggccat gcaattaaga tgttcaccgg
     5521 aatcgctacc aatctggaat aaaaagactg ttcgaagctc attccggaga ggaaaacttc
     5581 aatacacatg ttcacgacag aacagtaaac aaccatcgcg aacgtatctg caactgcatg
     5641 acgcaagcgt gactgcggtg agaacatgct aatgctcctt aataaaagcg taatgggatg
     5701 ttaatggaga tgaaagcagt ggatctcatt gccatctgca ttttcgcata gcgtatatcg
     5761 ctggctatga ctaatcaatt agtgaaaaac ttttatttag tcagtttttg tagataaaat
     5821 attccgtaac ggctcgtttt ttgtacattg tggattttta catgccgata tgat
//
LOCUS       DAPHJE010000195         5866 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPHJE010000195 DAPHJE010000000
VERSION     DAPHJE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.197"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(351..572)
                     /locus_tag="RH222_004245"
     CDS             complement(351..572)
                     /locus_tag="RH222_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799168.1"
                     /translation="MHFRKMKVVFLFIHVLAVVMISVMTVHLCSMAVEKVPEECDIPV
                     RVAALVVLWLVFSGLIYWLFTAFMKTDVM"
     gene            complement(587..778)
                     /locus_tag="RH222_004246"
     CDS             complement(587..778)
                     /locus_tag="RH222_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799169.1"
                     /translation="MSDFVRHFSVKDKKEDVVMQDMHFLLEDFICAHISRKSGRVPGR
                     LRQRMDKLRLQRIRKDRTG"
     gene            complement(891..1922)
                     /gene="f17g"
                     /locus_tag="RH222_004247"
     CDS             complement(891..1922)
                     /gene="f17g"
                     /locus_tag="RH222_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17G fimbrial adhesin"
                     /protein_id="HDV3799170.1"
                     /translation="MTNFYKVFLAVFILVCCNISHAAVSFIGSTENDVGPSQGSYSST
                     HAMDNLPFVYNTRYNIGYQNANVWRISGGFCVGLDGKVDLPVVGSLDGQSIYGLTEEV
                     GLLIWMGDTKYPRGTAMSGNSWENVFSGWCVGNYVSTQGLSVHVRPVILKRNSSAQYS
                     VQKTSIGSIRMRPYNGSSAGSVQTTVNFSLNPFTLNDTVTSCRLLTPSAVNVSLAAIS
                     AGQLPSSGDEVVAGTTSLKLQCDAGVTVWATLTDATTPSNRSDILTLTGASTATGVGL
                     RIYKNTDSTPLKFGPDSPVKGNENQWQLSTGTETSPSVRLYVKYVNTGEGINPGTVNG
                     ISTFTFSYQ"
     gene            complement(1924..4392)
                     /locus_tag="RH222_004248"
     CDS             complement(1924..4392)
                     /locus_tag="RH222_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3799171.1"
                     /translation="MLSVPLWAAEDAEFDPSFLSRTPGSSVIDVRRFTHGNPVPSGNY
                     YSDIYLNGEWKGNAELSFRDMSSSQEESVLCVTPELLSILDVSPEAVAREPDTGNGRC
                     IPFSAAVPDAKIRFDLSLLRLNVEIPQAMLVSRPRGYISPSQWQTGVPAAFVSYDVNH
                     WRYRTPDMENNQSYLGLRAGFNAGGWAFRHRGTESWSDGHAEGYRSIETNLQHDIAML
                     RAQLTLGDFTTTGELMDSNSLRGIRLTSDDRMLPGSLRGYAPVVRGMAGSNARVTVRQ
                     NGNIIYETTVPAGPFSISDLYPSGYGGDLTVTVTESDGQTRSFIVPFASVAQLVRPGF
                     SRWQVAAGRYRYGNRTFSDTVFQGTLQYGLTNDITLNTGMSTAPHYLSGLGGVAFNTP
                     LGAVATDITLARTTFPGSDTTHRGYSLHAGYSARIPETSTNVTLAAYRYSSRDFWSLR
                     DAILTRNRDVINDSSVRSATGYRPRNQLQLTVNQSLGEGWGNLYLTGATYHYWGHTGT
                     FNEYQAGYNNVWKKMSYQIGFSQTRRSGDYHHDNRFFFSFSLPLSDTGRTLLSTTVNV
                     SDKNSNSIQSSISGVSGDDNQLSYGVSLNTQENGPTGYAVNGSYRSPVATLQATAGND
                     SRHNRQISAGISGAIVAHPFGVTASNDLGDTFTVIHADGAGGAVINNAPGNRLDPWGN
                     GIVPYVTPYEKNYISIDPTALAADVELSATEREIIPRANSTTMVTFTTKTGKAMLFDV
                     KMPDGTPPPMAAEALTADGKSAGYIAQGGRLFVRDLDAEKGMLRIVWGSGQNDSCVIH
                     YSASGENARGNAVPENHHVVCMRQ"
     gene            complement(4476..5198)
                     /locus_tag="RH222_004249"
     CDS             complement(4476..5198)
                     /locus_tag="RH222_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3799172.1"
                     /translation="MKHILLFIFTVLLSLPSYGSVVIMGTRVIYPAEQKSINVRLNNG
                     DSTPSLIQAWLDTGDPSSPPDSVRVPFIITPPVFRMEPLSGQTMRIMYTGEKLPADRE
                     SLFWLNVLDIPAKPSFAGKSEKAQGYNYLQFAVRSRIKFFFRPDGLPFSPDDAYKKVT
                     WQLTGGDTLQASNPTPYFITFSTVSVGQGAQKVSSTQGGMAAPFSSVTFRLKQKVSPS
                     GKVEWTVVNDNGGYQKGESVVK"
     gene            complement(5266..5808)
                     /gene="f17a"
                     /locus_tag="RH222_004250"
     CDS             complement(5266..5808)
                     /gene="f17a"
                     /locus_tag="RH222_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_061363423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17A fimbrial adhesin"
                     /protein_id="HDV3799173.1"
                     /translation="MQKIQFILGILAAASSSATLAYDGTITFNGKVVDQTCSVTTESK
                     NLTVKLPTVSANSLASSGKVVGLTPFTILLEGCNTPAVTGAQNVNAYFEPNANTDYTT
                     GNLTNTASSGASNVQIQLLNADGVKAIKLGQAAAAQSVDTVAINDANVTLRYNAQYYA
                     TGVATAGDVTSTVNYTIAYQ"
BASE COUNT         1597 a         1545 c         1259 g         1465 t
ORIGIN      
        1 taatacagat aactgtaatt tatcattcag acgaagagat ttgatttaca cggtaagagc
       61 atatggcaca ttacaacagg ggtctctgtg aatatataca gaaaacatat ctcataaaac
      121 ccgggaaatg ccccatgaat acaggacagt gaaaacaggc atgataatca ggaaagccat
      181 gcctgataca gaaaaagtta cctgcgggga taatgcatgc gtattttcat ggaaggagat
      241 cactcaataa cttccatcga gagcgggtaa taactcaccg atctccttca cggtaataag
      301 caatgcacaa ccaggcactt actggccggt tatccatgtc tatattatat ttacatcacg
      361 tcagttttca taaaggctgt gaacagccag taaatcaggc cactgaatac cagccacagc
      421 acaaccaggg ctgcaaccct cacagggata tcacactctt ccggcacttt ttccacagcc
      481 atactgcaca gatggactgt cataacagaa atcatcacaa cagcaaggac atgaatgaaa
      541 agaaaaacga ctttcatttt ccggaaatgc atatacaaac cctgactcag cctgtcctgt
      601 ctttcctgat cctctgtaat cttaacttat ccatgcgctg acgaagtctg cccggaacac
      661 gcccgctctt tcttgaaata tgagcacaga taaaatcttc aagcagaaaa tgcatgtcct
      721 gcattaccac atcttctttt ttgtctttca ctgaaaaatg cctgacaaaa tcgctcatga
      781 aaatgtttct gaaacgctgt tatccagctt cagaaaccgg tactgccttc accaacacaa
      841 atcattttgt tgtcactgtc tgacctgttc tcccctcccg gaactcgctg ttactgatag
      901 gaaaacgtaa atgttgatat tccgttaacc gtacccggat taattccctc accagtattc
      961 acatacttta catacaaccg gactgagggt gacgtttccg ttcctgtcga taactgccac
     1021 tggttttcat ttccctttac cggcgaatca ggtccaaact tcaggggcgt actgtcagtg
     1081 tttttgtata ttctcagccc gactccggtt gcagtcgatg cccccgtcag tgtgagtata
     1141 tcgcttctgt tggacggtgt ggtcgcatca gtcagtgttg cccatactgt tactccggca
     1201 tcacactgta atttcagtga tgttgtcccg gcgacaactt catcaccgga tgatggtagt
     1261 tgtccggcag aaattgcagc caggctgaca ttgactgcgg aaggtgtcag taatctgcac
     1321 gatgttactg tgtcattcag cgtaaatgga ttcaggctga aattcactgt ggtctgaaca
     1381 ctgcctgcag atgaaccgtt atagggcctc attctgatac tcccgatact ggttttctgt
     1441 acactgtatt gcgcagagga atttcttttt aaaattaccg gtcttacgtg aacagacagt
     1501 ccctgcgttg atacataatt tcccacgcac catccggaaa agacattctc ccatgagttt
     1561 ccactcatcg cggtacccct gggatacttc gtgtccccca tccatataag gagtcccacc
     1621 tcctccgtca gcccataaat actctgcccg tcaagactgc caaccacggg taaatccact
     1681 ttcccgtcca gaccaacaca aaacccgcca ctaatacgcc agacatttgc attctgatat
     1741 ccaatgttgt aacgggtatt atagacaaat ggcaggttat ccattgcatg agtgctggaa
     1801 taagagccct gagacggtcc aacatcattc tccgtactgc caataaatga aactgccgca
     1861 tggctgatat tgcagcaaac cagaatgaat acagccagaa agaccttata aaaatttgtc
     1921 atattattgc ctcatgcata ctacatgatg gttttccgga acagcatttc ccctggcatt
     1981 ttctccggac gcactatagt gaatgacaca actgtcattc tgcccgcttc cccagactat
     2041 cctgagcatc cctttttcag catccaggtc acgtacaaat aaccggccac cctgagcaat
     2101 gtagcctgca gatttcccgt ctgctgttaa tgcttccgcg gccatcggcg gcggcgttcc
     2161 gtccggcatc ttcacatcaa acaacatggc tttccccgtt tttgttgtga aggtcaccat
     2221 ggtggtactg ttggcccggg gaataatttc ccgctctgtt gcagacagtt ccacatccgc
     2281 agccagcgct gtcgggtcga tactgatata attcttttcg taaggtgtga catacggaac
     2341 aatgccgtta ccccaggggt ccagccggtt acccggcgca ttattaatca ccgccccacc
     2401 ggcaccatca gcatgaataa cggtaaatgt gtcaccaagg tcattactgg ccgtcacacc
     2461 aaacggatgc gccactattg ccccggatat accggcagat atctgacggt tatggcgact
     2521 gtcattacct gccgtggcct gcagggtggc caccggtgaa cgataactgc cattcaccgc
     2581 ataccctgtc ggtccgtttt cctgggtgtt cagggacaca ccatatgaca actgattgtc
     2641 atctccgctg acaccactga tactgctctg aatgctgtta ctgtttttat cgctgacatt
     2701 cactgtcgtg gacagcaacg tccttcccgt atctgaaagt ggcagggaaa aactgaaaaa
     2761 gaaccggtta tcatgatgat aatcaccact ccgtcgggtc tgggaaaagc caatctggta
     2821 actcattttt ttccagacgt tgttataacc ggcctggtac tcattgaatg tccctgtatg
     2881 tccccagtaa tgataagtgg ctccggtcag atacaggttt ccccatccct caccaagact
     2941 ctggttcaca gtcagttgca actgattcct tggccggtaa ccagttgcac tcctgaccga
     3001 tgaatcatta atcacatcac gattcctggt caggatggca tcccgaagac tccagaagtc
     3061 acgggatgaa taacggtaag ccgccagcgt cacatttgta ctggtttccg gtattcgtgc
     3121 actgtaaccg gcatgcaggc tgtagccgcg atgtgtcgtg tcagaacccg ggaaagtcgt
     3181 tctggccagc gtaatatccg tggcaacagc ccccagtggt gtgttgaatg ccacaccacc
     3241 cagccctgac agataatgag gtgcggtact catccccgta ttcagggtga tatcatttgt
     3301 cagcccgtac tgcagggtcc cctggaatac ggtatcgctg aatgtcctgt taccgtaacg
     3361 ataacgcccg gctgccacct gccagcgact gaaccccggg cgaaccagtt gtgcaaccga
     3421 agcaaacggc acgataaaac tcctggtctg tccgtctgac tctgtcacag tgactgtcag
     3481 gtcaccacca tatccactgg gataaaggtc actgatggag aaaggccctg ccggcacggt
     3541 agtttcataa ataatgttac cgttctgccg gacagtgacc ctggcgttac ttccggccat
     3601 cccccgaacc acaggggcat aaccacgcaa cgaacccggc agcatccggt catcagaagt
     3661 cagtcttatt cccctgaggc tgttactgtc cataagctca cctgtggtgg tgaaatcccc
     3721 cagtgtcagt tgcgcacgca acatggctat gtcgtgctga aggttcgttt caatactccg
     3781 gtaaccttct gcatgaccgt cactccagct ttctgtcccc cggtgacgaa atgcccagcc
     3841 tcctgcgtta aagccggcac gaagaccaag ataactctga ttattttcca tatcgggcgt
     3901 ccggtaacgc cagtgattca cgtcataact gacaaaggca gccggcaccc ccgtctgcca
     3961 ctgagaaggt gatatgtatc cccgtggacg ggaaaccagc atggcctgcg gaatttcaac
     4021 attcagccgc agcagtgaca ggtcaaaacg gatttttgca tcaggtacag ctgcagaaaa
     4081 tggtatgcaa cggccatttc cggtatccgg ttcacgggca acggcctcgg gtgacacatc
     4141 cagaatggat aacagttccg gcgtcacaca cagaacactc tcctcctgtg aagaggacat
     4201 atcacggaat gacaattccg catttccttt ccattcaccg ttcagataaa tgtctgaata
     4261 ataattccct gagggaacag gattcccgtg cgtgaaacga cggacatcaa tcacactgct
     4321 ccctggcgtg cggctcagaa aactcgggtc aaattcagca tcctctgccg cccacaaagg
     4381 cacagacagc atatacagtc ccgacagaca acataaataa atgacacggt acttcagacc
     4441 ggataaaaaa taatgactca cttacggggc ccttattatt tcacaacgga ttcgcctttc
     4501 tggtaaccac cattatcatt caccaccgtc cattccactt ttcctgacgg agaaaccttc
     4561 tgcttaagtc tgaaagtgac agaagaaaac ggtgcggcca ttcccccctg agtactgctg
     4621 actttctgag ctccctgccc gacactgaca gtactgaagg taataaaata aggcgtggga
     4681 ttactggcct gaagggtatc accgcctgta agctgccatg tcactttctt atatgcatca
     4741 tcaggagaaa aaggcagacc atcagggcgg aaaaaaaact taatccggct tcgtacggca
     4801 aactgaaggt aattatatcc ctgtgctttt tcagattttc cggcaaaaga aggtttggcc
     4861 ggaatatcaa gaacattcag ccagaacagt gattccctgt ctgccggaag tttttctccc
     4921 gtatacatga ttcgcattgt ctgtccggaa agcggctcca tccggaaaac aggaggcgta
     4981 ataataaacg gcaccctgac agagtcagga ggggaagacg gatcccctgt atcaagccat
     5041 gcctgtatca gggatggcgt actgtctccg ttattcagcc ggacattaat acttttttgt
     5101 tctgcaggat aaataaccct ggtccccata ataaccacac tgccatatga gggcagagac
     5161 aaaagaacgg taaaaatgaa cagcagaata tgtttcatac aggctccgta aacacggggc
     5221 aaaatgcccc gtgaacactt aagaaggcag gtattaagat aaaaattact gataagcgat
     5281 ggtgtaatta actgtagaag taacatcgcc tgcagtagcc acacccgttg cgtagtactg
     5341 cgcgttataa cgcaatgtca catttgcatc attaatagca actgtatcca cactctgagc
     5401 tgcagcagcc tgaccaagtt taatagcttt aaccccatct gcattcagta gctgaatctg
     5461 aacattagat gcaccagaag aagccgtatt agttaaatta ccagtggtgt aatccgtgtt
     5521 cgcattaggt tcgaaataag catttacatt ctgagcacct gtcacggcag gcgtattgca
     5581 cccttccagc aaaattgtga aaggagtaag tcccaccact tttccgcttg aagctaatga
     5641 attagcagag acagttggta acttaactgt caaattcttg ctttctgttg taacagaaca
     5701 agtttgatca acaacttttc cattaaaagt aattgtaccg tcataagcaa gcgtagcaga
     5761 agatgatgca gccgccagta ttccaaggat aaattgaatt ttctgcatag aatcctcagt
     5821 gaaaaccaat aagaatatat catgaaaaca gcagcaatta agaatt
//
LOCUS       DAPHJE010000196         5855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPHJE010000196 DAPHJE010000000
VERSION     DAPHJE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.198"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..249
                     /locus_tag="RH222_004251"
     CDS             97..249
                     /locus_tag="RH222_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV3799174.1"
                     /translation="MAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGFDIPGKIY
                     KLSVAK"
     gene            261..1379
                     /locus_tag="RH222_004252"
     CDS             261..1379
                     /locus_tag="RH222_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3799175.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            1422..1547
                     /locus_tag="RH222_004253"
     CDS             1422..1547
                     /locus_tag="RH222_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799176.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(1600..1857)
                     /locus_tag="RH222_004254"
     CDS             complement(1600..1857)
                     /locus_tag="RH222_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799177.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            1847..2083
                     /locus_tag="RH222_004255"
                     /pseudo
     CDS             1847..2083
                     /locus_tag="RH222_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2171..3338
                     /locus_tag="RH222_004256"
     CDS             join(2171..2424,2423..3338)
                     /locus_tag="RH222_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3799178.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEILKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAVASFC"
     gene            complement(3273..3686)
                     /locus_tag="RH222_004257"
     CDS             complement(3273..3686)
                     /locus_tag="RH222_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HDV3799179.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLLKWSTKTGHRVRVFPVSIFRFVWG"
     gene            complement(3749..5746)
                     /gene="betT"
                     /locus_tag="RH222_004258"
     CDS             complement(3749..5746)
                     /gene="betT"
                     /locus_tag="RH222_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV3799180.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVSLALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
BASE COUNT         1686 a         1319 c         1404 g         1446 t
ORIGIN      
        1 gcagggttgg ttccaaaatt tatatatgat tactttccaa caaaattaag aggattaggg
       61 accggtctta tttataactt aggggcaact ggaggaatgg ccgcacctgt attagctaca
      121 tacatttcag gatattatgg cttaggtgtt tcattattca ttgttacggt tgcattctct
      181 gccttattaa ttttgttagt tggttttgat attccaggta aaatttataa actatccgtg
      241 gctaaatgat tggaggcttt atgattaatt atggcgttgt tggtgttgga tactttggcg
      301 ctgaattagc tcgttttatg aatatgcatg ataatgcaaa aattacatgt gtatacgatc
      361 ctgaaaatgg agaaaatatt gcccgtgaat tgcagtgtat caatatgtca agcttggatg
      421 ctttagtctc aagtaaatta gtcgattgcg tgatcgtagc caccccaaat tatctgcata
      481 aagaaccagt aattaaagca gcaaagaata agaagcatgt tttttgtgaa aaaccaattg
      541 cattaagtta tgaagattgt gtggatatgg tcaaagcgtg taaagaagct ggtgtgacct
      601 ttatggccgg gcatattatg aattttttca atggggttca atatgcacgg aagttaatta
      661 aagaaggtgt tatcggcgaa atattatcat gtcatactaa gagaaatggc tgggaaaaca
      721 aacaagagag actttcctgg aaaaagatga aagaacaatc tggtggacat ctatatcatc
      781 atatacatga gttagattgt gttcagcatt tacttggaga aataccagag acggttacta
      841 tgattggtgg aaatttggcc cattctggtc caggatttgg caatgaagat gatatgttat
      901 ttatgacctt ggaattcccg tcaggaaaac tagcaacctt agagtggggg agtgcattta
      961 actggccgga acattatgtc atcatcaatg gaactaaagg ctctattaaa attgatatgc
     1021 aagaaacagc agggtcactt aggattggcg gtcagacaaa gcattttttg gtccatgaaa
     1081 cacaagaaga agatgatgat cgtcggaaag gcaatatgac ctcagaaatg gatggcgcta
     1141 tagcatatgg tcatccagga aaaaaaacac cattatggct tgccagttta ataagaaagg
     1201 agacgttatt cctccataat atcctctgtg gtgcaaaacc tgaagaagat tatattgacc
     1261 ttctcaatgg tgaggcggcc atgtcggcga ttgctactgc tgatgctgcc actctttcaa
     1321 gatcgcagga caggaaagtg aaaatcagtg agatcattaa acatacatca gtaatgtaaa
     1381 aactatacgc ctcttgattt aattcaggag gccttttatg tatgatcaag aattttatct
     1441 tcgataatct cataatttta gcagtgccat ttatgataaa aacatccctt aaaaccaatc
     1501 taatcttttt cttcctctgt gtatttgttc cccatatggc gtcataattt acgctacgta
     1561 atacgggata gttacgatac gcagcgatag cgctaagttt tagttaaaat cccccatcag
     1621 cgggatatgg ggatctctgg gaggttcagg ttttagcatc gcgactttaa tacgacagac
     1681 gctggtgacg atgatgccaa gtagtgactc ttccgaataa ggttcccact gaccaattgg
     1741 cgcgcgcttc gcaataaaat ctcccttcgc ccagtattgg gaaaagtaga tacattcaaa
     1801 ctgtgtacgc tgtttcgtct cacagttcac gacattgctg gccgatgagc tgaccattgg
     1861 gcctatccgg gctgtcccga tggatattac gccgaagtat gtgggaattg ccagcggatt
     1921 gatgaacgcc ggttccgctg tcgccgacat tatttcacct atcgcctttg gcattattat
     1981 cgacaaaaca ggcaactgga gtttaccatt ctacggttcc gtcgcattgc ttgttattgg
     2041 tatattcctg acgttcttca tgcgtccaga taagtcattg taaaagtcga atcagggctg
     2101 aagtggcaca ctgaatttgg ccacctgaac agaggtgata tgctcacctc agaacaacac
     2161 aggtgctcca atgaaaaaaa gaaatttcag cgcagagttt aaacgcgaat ccgctcaact
     2221 ggttgttgac cagaaataca cggtggcaga tgccgcgaaa gctatggatg ttggcctttc
     2281 cacaatgaca agatgggtca aacaactgcg tgatgagcgt cagggcaaaa caccaaaagc
     2341 ctctccgata acaccagaac aaatcgaaat acgtaagctg aggaaaaagc tacaacgcat
     2401 tgaaatggag aatgaaatat taaaaaggct accgcgctct tgatgtcaga ctccctgaac
     2461 agttctcgat aatcgggaaa ctcagagcgc attatcctgt ggtcacactc tgccatgtgt
     2521 tcggggttca tcgcagcagc tacagatact ggaaaaaccg tcctgaaaaa ccagacggca
     2581 gacgggctgt attacgcagt caggtacttg agctacatgg catcagccac ggttcggccg
     2641 gagcaagaag catcgccaca atggcaaccc ggagaggcta ccagatggga cgctggcttg
     2701 ctggcaggct catgaaagag ctggggctgg tcagctgtca gcagccgact caccggtata
     2761 aacgtggtgg tcatgaacat gttgctatcc ctaactacct tgaaaggcag ttcgccgtga
     2821 ccgagccaaa tcaggtgtgg tgcggtgatg tgacctatat ctggacgggt aagcgctggg
     2881 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
     2941 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
     3001 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
     3061 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
     3121 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
     3181 gttacgtaag cttcagcgat gcagctcacg caataacgga ctatatcgtt ggatattaca
     3241 gcgcactaag accgcacgaa tataacggtg ggttaccccc aaacgaatcg gaaaatcgat
     3301 actggaaaaa ctctaacgcg gtggccagtt tttgttgacc acttcaagag aaaagaaccg
     3361 atgaaggtgg ccagtacatt ttgtgtggtg acgtcttcaa caactaaacg cgtagctctg
     3421 ggagtcacat tagtagtggc tgaaccgtag gctgtgacca tgatactcag cgaaaatgtg
     3481 gtcactgcca gcatactcga tgccagtatg ttcagaatgt tatcgactgc ttccgcacca
     3541 accttcacgg aaaccgactc aggtatcatt gatttaaaaa gaattgataa aagggccgtt
     3601 attatcgcga caattgcgaa taacgttgcc ctgaaccata gttttttaat tgtctgcttc
     3661 agcatccatt tccagcgtga aatcattctg cattccctca tattgcggcc gagataataa
     3721 ttgccgcccc caggacggca tgttgctatt aacgaaccag gtgcaggaag tgcaggtgtt
     3781 tttcgtactg gtccagtatg tcattaatca gctgttcctg gttccagccc atcacatcat
     3841 aattttgacc gccttctttg agataaacct cagcgcgata atatcgatgt tgttcagtct
     3901 gctgctcatc attatccatt gcggcgagcg cgaaggtcgg tgagatatac ccgcgaagcc
     3961 tcacttcata tataaaattc agctcgttgc ccaaatcgac ttcaagacga atacgatcgt
     4021 cgactgcatc actaatgtgg cttatcgtcc cctgcttgtt cagttcctcc tgaaccagcg
     4081 tcatggcggg ctggataacg tcgtccataa aacgtttcac aagagatcgc ttcggcagat
     4141 acgcgatatt gcgtaacctt ctctgccaag gaattgggtt acgtgcagcc gtaggagcaa
     4201 ttgtcgccat gctcaggctt tcacgcttgg tcaaatcccg gcgcaaagct tttaaaagtc
     4261 cgtatatgga tattagtaag atcactgaga agggcaatgc actcgctatt gtcaccgttt
     4321 gcagcgcact tagccctccg gcaaggagaa gcgcaattgc aacaatgccc atgagcgagg
     4381 cccagaatat tcgctgccag acgggtgtgt ttgccactcc acctgatgcc agagtatcca
     4441 caaccattgc ccccgaatca gcagacgtta caaagaagac gatgaccatc gccattgcaa
     4501 tgaatgacag cacagaagag aacgggaaat gctccaggaa attaaacagg gccagcgaca
     4561 catcctgctg aacagtattg gcgaggtctg tggccccctg gttcataatg agatagatcg
     4621 cgctgttacc aaacaccgtc atccacatta gcgtaaaacc cgcgggaaca aacagcacgc
     4681 cggtgacaaa ctcgcgaatg gttcgcccgc gggagacccg tgcgatgaac atccccacaa
     4741 acggcgacca tgaaagccac catccccagt acagtaatgt ccagcccccc agccagttgc
     4801 tcgacttggg ctcatacgcg taaaggttga acgttttact caccagttcc gaaagataac
     4861 cgcccgtatt ttccacaaat gacttcagca gaagcacggt tggtcccaga cacaggacca
     4921 gcgccaggag caacaaagcc agacccagat tgagttcaga caggatacgt attcccttat
     4981 ccagaccgga caccactgaa atcgtcgcta accccgtgat gaccacgatc agaattacct
     5041 gcaccgtttc attgatgggc accccgaaaa gatggttcaa accggcattc acctgcaaaa
     5101 caccgtaacc cagtgatgtc gcaacgccaa agaccgtgcc tataacagcg aaaatatcaa
     5161 ccgcatgtcc tacaggtccg tatatgcgat cgccaataat gggatagagt gcggagcgca
     5221 gagttaaagg cagaccgtga cggtaactga agaacgccag aatcagcgcc acaatggcat
     5281 aaattgccca tgcgtgcagt ccccagtgga aaaaggtcag acgcattgct tccttggctg
     5341 ccgcaacggt ttctggagtg ccaacgggtg gcgaaagata atgcattaca ggttcggcaa
     5401 cgccaaagaa catcaggccg atccccatcc ctgccgaaaa aagcatcgca aaccaggagt
     5461 ggtagctgaa atcaggctgc gcatggtccg ggcccagctt gatatcaccg tagcgtgaga
     5521 gtccaaggaa cgtgacactc agtaaaatca gggccacagc aaggatgtag aaccagctgg
     5581 cattcgtgaa gatttgttgc tgaagtagtt taaaattttt gtcggcgaca tccgggaata
     5641 cggcggcaaa ggcgacaaga aggaaaatta gcaaagcaga tgtaaagaat accgctttgt
     5701 taatctgact tgtagacttc tttgggattg tatcattttc actcataatc atttattcca
     5761 ttaattaaat tcgcactgga tagaaggtac tacctctcac aaagtagcaa agttcaactg
     5821 gctatgcggc gttaatcgga atgcatggtg gaatg
//
LOCUS       DAPHJE010000197         5805 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPHJE010000197 DAPHJE010000000
VERSION     DAPHJE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.199"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            455..2740
                     /gene="nrdA"
                     /locus_tag="RH222_004259"
     CDS             455..2740
                     /gene="nrdA"
                     /locus_tag="RH222_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV3799181.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2975..4105
                     /gene="nrdB"
                     /locus_tag="RH222_004260"
     CDS             2975..4105
                     /gene="nrdB"
                     /locus_tag="RH222_004260"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV3799182.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4105..4359
                     /gene="yfaE"
                     /locus_tag="RH222_004261"
     CDS             4105..4359
                     /gene="yfaE"
                     /locus_tag="RH222_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV3799183.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4413..5063)
                     /gene="inaA"
                     /locus_tag="RH222_004262"
     CDS             complement(4413..5063)
                     /gene="inaA"
                     /locus_tag="RH222_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV3799184.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5242..5484
                     /locus_tag="RH222_004263"
                     /pseudo
     CDS             5242..5484
                     /locus_tag="RH222_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1472 a         1515 c         1394 g         1424 t
ORIGIN      
        1 atttaacaat aaccgaatag aaaacaacca tttcgccatc aacaatctct tacattcgct
       61 tatatattga ccacaactga tacatcagat tatgtgatga ctcgtgctta gatcaatttt
      121 tgcaatcatt agcaaaaaga ttaataaacc atctatatca atttatctaa cctattatgc
      181 cgttcaagaa atcgccgaac agttattttt aacaaatttt tctcttccca ttgactttcc
      241 cggacacctt gtctgaccta aggtgcgcga aagccacttt ttccttcctg agttatccac
      301 aaagttatgc acttgcaaga gggtcatttt cacactatct tgcagtgaat cccaaacata
      361 ccccctatat atagtgttct aagcagcttc ccgtactaca ggtagtctgc atgaaactat
      421 tgcggaaaga attccaaaaa caggtacgac atacatgaat cagaatctgc tggtgacaaa
      481 gcgcgacggt agcacagagc gcatcaatct cgacaaaatc catcgcgttc tggattgggc
      541 ggcagaagga ctgcataacg tttcgatttc ccaggtcgaa ctgcgctccc acattcagtt
      601 ttatgacggt atcaagacct ctgacatcca cgaaaccatt atcaaggctg ccgcagacct
      661 gatctcccgt gatgcgccgg attatcagta tctcgccgcg cgcctggcaa tcttccacct
      721 gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg tacgaccatg tggtgaaaat
      781 ggtcgagatg ggcaaatacg ataatcatct gctggaagac tacacggaag aagagttcaa
      841 gcagatggac acgtttatcg atcacgaccg tgatatgacc ttctcttatg ctgccgttaa
      901 gcagctggaa ggcaaatatc tggtacaaaa ccgcgtaacc ggcgaaatct atgagagcgc
      961 ccagttcctt tatattcttg ttgccgcgtg cctgttctcg aactacccgc gtgaaacgcg
     1021 cctgcaatac gtgaagcgtt tttacgacgc ggtttccaca tttaaaattt cgctgccgac
     1081 gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg tactgatcga
     1141 gtgcggtgac agcctggatt ccatcaacgc cacctccagc gcgattgtga aatatgtttc
     1201 ccagcgtgcc gggatcggca tcaacgccgg acgtattcgt gcgctgggca gcccgattcg
     1261 cggtggcgaa gcgttccata ccggctgtat cccgttctac aaacatttcc agacagcggt
     1321 gaaatcctgc tctcagggcg gtgtgcgcgg cggtgcggca acgctgttct acccgatgtg
     1381 gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac cgtggggttg aaggcaaccg
     1441 cgtgcgtcat atggactacg gggtacaaat caacaaactg atgtataccc gtctgctgaa
     1501 aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg acgcgttctt
     1561 cgccgatcag gaagagtttg aacgtctgta taccaaatat gagaaagacg acagcatccg
     1621 caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac gtgcgtctac
     1681 cggtcgtatc tatattcaga acgttgacca ctgtaatacc catagcccgt ttgatccggc
     1741 catcgcgcca gttcgccagt ctaacctgtg cctggaaatt gccctgccga ccaaaccgct
     1801 gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg ctttcaacct
     1861 gggcgcaatt aatagcctgg atgaactgga agagctggca attctggcgg ttcgtgcact
     1921 tgacgcgctg ctggattatc aggattaccc gatcccggcc gccaaacgtg gagcgatggg
     1981 tcgtcgtacg ctgggtattg gtgtgatcaa cttcgcttac tacctggcga agcacggtaa
     2041 acgctactcc gacggcagcg ccaacaacct gacgcataaa accttcgaag ccattcagta
     2101 ttacctgctg aaagcctcta atgagctggc gaaagagcaa ggcgcgtgcc cgtggtttaa
     2161 cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc tataagaaag atctggatac
     2221 catcgctaat gagccgctgc attacgactg ggaagctctg cgtgagtcaa tcaaaacgca
     2281 cggcctgcgt aactccacgc tttctgctct gatgccgtcc gagacttctt cgcagatctc
     2341 taacgccact aacggtattg aaccgccgcg cggctacgtc agcatcaaag cgtcgaaaga
     2401 cggtattctg cgccaggtgg tgccggacta cgagcatctg cacgacgcct atgagctgct
     2461 gtgggaaatg ccgggtaacg atggctacct gcaactggtg ggtatcatgc agaaatttat
     2521 cgatcagtcg atctctgcta acaccaacta cgatccgtca cgcttcccgt caggaaaagt
     2581 gccgatgcag cagttgctga aagacctgct caccgcctac aaattcgggg tcaaaacgct
     2641 gtattatcag aacacccgtg acggcgctga agacgcgcag gacgatctgg tgccgtcaat
     2701 ccaggacgat ggctgcgaaa gcggcgcatg taagatctga tattgagatg ccggatgcgg
     2761 cgtaaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac gcgccagcgt
     2821 cgcatcaggc tccgggtgcc ggatgcagcg tgaacgcctt atccggccta cggctcggat
     2881 ttgtaggcct gataagacgc gtcagcgtcg catcaggcac aggatgcggc gtaaaatgcc
     2941 ttatccggca ttaaactccc aacaggacac actcatggca tataccacct tttcacagac
     3001 gaaaaatgat cagctcaaag aaccgatgtt ctttggtcag ccggtcaacg tggctcgcta
     3061 cgatcagcaa aaatatgaca tcttcgaaaa gctgatcgaa aagcagctct ctttcttctg
     3121 gcgtccggaa gaagttgacg tttctcgcga ccgtatcgat taccaggcac tgcctgaaca
     3181 cgaaaaacac atctttatca gcaacctgaa atatcagaca ctgctggatt ccattcaggg
     3241 ccgtagcccg aacgtggcac tgctgccgct gatctcgatc ccggaactgg agacctgggt
     3301 cgaaacctgg gcgttttcag aaacgattca ttcccgttcc tatactcata tcattcgtaa
     3361 tatcgttaac gatccgtctg ttgtgtttga cgatatcgtc accaacgagc agatccagaa
     3421 acgtgcggaa gggatctcca gctattacga tgagctgatc gaaatgacca gctactggca
     3481 tctgctgggc gaaggtaccc acaccgttaa cggtaaaact gtgaccgtta gcctgcgcga
     3541 gctgaagaaa aaactgtatc tctgcctgat gagcgttaac gcgctggaag cgattcgttt
     3601 ctacgtcagc tttgcttgtt ccttcgcatt tgcagaacgc gaattgatgg aaggcaacgc
     3661 caaaattatt cgcctgattg cccgcgacga agccctgcac ctgaccggca cccagcatat
     3721 gctgaatctg ctgcgcagcg gcgcggacga tcctgagatg gcggaaattg ccgaagagtg
     3781 taagcaggag tgctatgacc tgtttgttca ggcagctcaa caggagaaag actgggcgga
     3841 ttatctgttc cgcgacggtt cgatgattgg tctgaataaa gacattctct gccagtacgt
     3901 tgaatacatc accaatatcc gtatgcaggc agtcggtttg gatctgccgt tccagacgcg
     3961 ctccaacccg atcccatgga tcaacacctg gctggtgtct gataacgtgc aggttgctcc
     4021 gcaggaagtg gaagtcagtt cttatctggt cgggcagatt gacgcggaag tggacaccga
     4081 cgatttgagt aacttccaac tctgatggcc cgcgttaccc tgcgcatcac tggcacacaa
     4141 ctgctgtgcc aggatgaaca cccttccctt ctggcggcgc tggaatccca caatgtggcg
     4201 gttgagtatc agtgtcgcga aggttactgc ggctcctgtc gcacacgcct ggttgcaggt
     4261 caagttgact ggattgccga accgttagcc tttattcagc cgggggaaat tttgccctgt
     4321 tgttgccggg caaaaggcga tattgaaatc gagatgtgaa ttggttgtag tgccagatac
     4381 aacgcttatg cgtcttatct ggcctacaac gattacatgg cgtagtaata cgctttcacc
     4441 tgctcccagt ccgctttggg gattggctcc agatattttt ccagctggcg gaagtcatga
     4501 ttaatcgctt tatcgcgacg caagcggcga cgacttttct ccaggtcaag aaaaccagct
     4561 tctgccttac cttctgtttt cacatagata tggcgaacat aacaacagcc atgctgacga
     4621 ttaatgctat gcattttctt aaacgccagc gccactgctt tcaacatggc ttgccgtact
     4681 tcgtcagaat aaggcgatac tgcatgctgg gcataccagt cagcaatgct gatgaacccc
     4741 gccatatctt cagtcaccag caacgctcgc cattcaccct caattttcac cgcttcacca
     4801 aaaacgatct tcggcacgat gacaccggcc cgttccagtt ctttaattac cgcaacctca
     4861 cggacaatcg ttggtcggcc gaacggataa cgtacggaat gaaacagatg atgcgtcatg
     4921 cgctttacat acagcttttt gccgttgcgc tcgacgcatt gcaccccgct cataccatta
     4981 cggcgatagt taggctcttc aacccagtcg ccctctgttg cccaccagtg attaaattcg
     5041 tcgtactttg ctgaaactgc cataccctat cgcctgtcat ttttattaac gacaatgact
     5101 ataggtggtt acctgaggaa aatcttaatg aaacgtgtcg tattaatgaa tttcttttgc
     5161 cactactagc gtgacaccgc ttttaccctt catttgcagc gcatagattc caccagccac
     5221 taccactaat agtgcgccca gcagataaaa tgtgaattta atcaggcgga tacagcgatg
     5281 aattttattc gacagggatt aggcatcgcc ttacaaccag agttaacgct gaaaagcatt
     5341 gcaggtgaat tgtgttccgt tcctctcgaa tcgaccttct atcgacagat ttcgttgttg
     5401 gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaacgtg tacggaacaa
     5461 ctcgtggtga gtggaaaaat ttgatgtgag acatgagatt gatgacaaat gtaagattgc
     5521 ctgattcgcc acacttagtt ggcctgtagt ctttaccagg cgtaataatt cgttttcacc
     5581 tgattttggt ccactttgtg aattagcgat atatatttat ccagtttagg gaagtgacaa
     5641 tgactccagg tagaaaccgg tatgaagcgc ccgactcact gtcaaaaccg attgttgaga
     5701 gtaatgacac tccggcgaac agtacacagt ttactctgga gccattctca atcttcaagt
     5761 gggataccta ataacttgtc agggaagtta tcaatatctg tttgt
//
LOCUS       DAPHJE010000198         5683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPHJE010000198 DAPHJE010000000
VERSION     DAPHJE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.200"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..1233
                     /locus_tag="RH222_004264"
     CDS             16..1233
                     /locus_tag="RH222_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3799185.1"
                     /translation="MQKSFRFTNSSIKALPANTDTRSTELEVSDTEVIGLKCLSGRTG
                     NKRFLLRYTFHGTKKSISIGRFPEIDVGTARQIARRHKEAIALGNDPKAERDNYRAMP
                     TLSEFFHLTYVPFIKRHKKSWDKDVQRFTQYIEPRLGKIRYCDLRAREIQQVLFDMLE
                     GRIHGQQYAPSTCNRALAILKTMGRYALRQDVLEKNEADKIPLLKENNQRTRFFDADE
                     IRRILDAAARYPNKAAGGLIAMLLLTGTRKSEMLNVMHKHIDRDNRSLFIPYTKNGRS
                     RTVYLSDVALSIIDSIPRAGSNPYLFAVKDNGKPISEPRWAYEKILAECGIDKSEVCF
                     HTTRHSVASLLVSSGQYSLYDVKAQLAHASIQSTERYAKLTPERMRQTGQGVTDLLLN
                     TVTAGNNDDFSTP"
     gene            1651..1965
                     /locus_tag="RH222_004265"
     CDS             1651..1965
                     /locus_tag="RH222_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799186.1"
                     /translation="MSLLKTELVLPFEDEYSAMCFWDEVGVTLIYVEPDDVPEDIKAQ
                     VLNVIHQLIANPEFVVRLTDDYYLMLSVTEDNGNGIYLLFHPDCPLKGIDLLISVVES
                     RY"
     gene            1991..2524
                     /locus_tag="RH222_004266"
     CDS             1991..2524
                     /locus_tag="RH222_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799187.1"
                     /translation="MSKSKLVKNNVESVSKEIKNEAEVIHENLSADEQTLSDVKDYQL
                     LIETGQAEKISPKASGMLTWQLAISEVDNELYLRLVANGCGGLFSKDWISLSKIASVL
                     ETQPATGFSSGVFRPLFKGVSANNAGFLAAVLRSPDICLLEAHPEKLFTHVLYPDWRE
                     RLNKLNTISSQEKITED"
     gene            2597..3031
                     /locus_tag="RH222_004267"
     CDS             2597..3031
                     /locus_tag="RH222_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2787 domain-containing protein"
                     /protein_id="HDV3799188.1"
                     /translation="MQTQYALKQAGLTLPVTKEFQQHITTLLANQVLQKITEAVVINF
                     RDPDYSTESGGFHPVEIRFIRKNNEWYFDYVTDFSYMGRVYPELEKEIDFCWSGNYVF
                     HYLIGDISLAAERNELWSLWERNFMEYLSMGIYRVTVTVESC"
     gene            complement(3642..4460)
                     /locus_tag="RH222_004268"
     CDS             complement(3642..4460)
                     /locus_tag="RH222_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HDV3799189.1"
                     /translation="MKKCPVLLVVSGFLTENDISWFNRIKAGCGLDVRYYKWESANLL
                     SILSSDIANKWGLYTLFPLRTSPLLSSISIPFKARQAWYNATKAAVNNREKLAHVINE
                     IYVKEKRNVIIMGHSLGTRLINNTLPYIKNDNILLTISMAGATPITSYTENIMKMAYG
                     SKMGHLNIYSEHDKVLNTIYPLVESVTPIGVREVRFRPARVNNWKWDIGHTEYITSNR
                     IDSLTHWLNVTCADLADPDVELTDDDIKLFNDIIDLANLTCHKNTVAPKLISFE"
     gene            complement(4522..5424)
                     /locus_tag="RH222_004269"
     CDS             complement(4522..5424)
                     /locus_tag="RH222_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HDV3799190.1"
                     /translation="MGKHDKLGYRLGLILTRLNNGESLAVRELSEEFNVCEKTIRRDL
                     TQRLSYLNLIRQNGRYRLSDGVLGQRSNADLRHFTRILGIEGLFPRWDDRLLSILLGN
                     TKNNPFLIKQRPYENCGSFMSILNVLSDAILSQKKVNFNYKDKEFRAVEPYRLVNDNG
                     LWYLAAAHDSTLKSFVISSVKDVCMSNISFRIIPEINEKIEKTDGIWYSEDLIEVLIS
                     VSAHVAPWFTHRHLLPGQEIIHTSRSGDLLVISRVTHTDQIMPLMKYWIPDVEVIQPA
                     SIRQQLAEDIQSALKRYTQPSNAP"
BASE COUNT         1638 a         1141 c         1263 g         1641 t
ORIGIN      
        1 tacatgagga taaatatgca aaaatccttc cgattcacca attcgtccat caaagccctt
       61 ccggcaaaca cagataccag atctacagag cttgaggtat ctgatactga ggtcattggc
      121 ctcaaatgcc tctcaggtcg aacgggtaac aaacgtttcc tcctgcgcta cacattccac
      181 ggtaccaaaa aaagtatcag tattggcaga ttccctgaaa ttgatgtggg gacagcccgt
      241 cagatagccc gtcgtcacaa ggaggcgatt gcactgggaa atgaccctaa ggcagagcgc
      301 gataattatc gtgcgatgcc aacgcttagt gagttttttc acctgaccta tgttccgttc
      361 atcaagcgcc ataaaaagtc ctgggataag gatgttcagc gtttcacgca atacattgaa
      421 cctcgtctgg ggaagatacg ttactgcgac cttcgggccc gggaaatcca gcaggtgctt
      481 ttcgacatgc ttgagggtcg tattcacggc cagcagtatg caccgtctac ctgtaacagg
      541 gcactggcta ttctgaaaac tatggggcgt tatgcgctac gacaggatgt cctggagaag
      601 aatgaagccg ataagatccc tttgctgaag gagaataatc agcggacgcg cttttttgac
      661 gccgatgaga tcagaaggat cctcgacgct gcggcacgat accccaataa ggcagccggg
      721 ggactgatag ccatgctgct gttaaccggt acacgcaaat cagaaatgct gaatgtcatg
      781 cacaaacata ttgatcggga taacaggtcg ttattcattc cttatactaa gaatggtcgc
      841 agcagaactg tgtatctcag tgatgttgca ttatccatta ttgattcgat cccacgggct
      901 ggcagcaacc cctatctgtt tgccgtaaaa gataatggaa aaccaatatc agaaccccgg
      961 tgggcctatg aaaaaatact ggcagagtgc gggatagata aaagtgaagt ctgtttccac
     1021 accacgcgcc attctgtcgc atcattactt gtctccagcg gtcagtacag tctgtatgac
     1081 gttaaagcac aactggcgca tgcgagtatt caaagtacgg agcgctacgc gaaattgaca
     1141 ccagagcgta tgcgtcagac cgggcagggc gtaactgacc ttcttttgaa caccgttact
     1201 gccggtaaca atgatgattt tagcacccct tgaacgtgga gaatgagtat gccgttaaaa
     1261 tgtcccaagt gtggtagccg aaatacagtg acagaaactg ccggaaagat agcagaagtg
     1321 actcgcgatg accggttcct gacatcgaca tccggttata ttagcccgga tcaattacca
     1381 gaattgctta aagaaatcat cagggcgata caacgtctct ttagattcct tgaacagcgt
     1441 gaaaggaata atgcgccagt gctgatctgc aaggattgtg gatattatga aagaatatag
     1501 atgaccactg aaggcaattc cagattcttt agcctgtcta tgtaaccccg atacttaaaa
     1561 cgtatcgggt tttttgttta tgaggattat tctatgaaat ttgtctcgaa ctggcaagac
     1621 ctgccgtcgc tccttcttcc ggagtcagta atgagtctgt tgaaaacaga gctggtactc
     1681 ccatttgaag atgaatactc tgcaatgtgt ttctgggatg aagtcggggt gacattaata
     1741 tatgtcgagc ccgatgatgt acctgaagat attaaagcgc aggtattaaa tgtgattcat
     1801 cagttaattg caaatccaga atttgtagtc cgtctgacag atgattatta cctgatgctt
     1861 tctgttacgg aagataacgg gaacggtatt tatctgttat ttcaccccga ttgccctctg
     1921 aagggtattg atttattaat ttctgtggtc gaatcacgct attaaacaca agcaaaacta
     1981 ggaacgcaaa atgagtaaat caaaattagt taaaaataat gttgagtcag tttcgaagga
     2041 gattaaaaat gaggcagagg ttattcatga aaatctcagt gcagatgaac aaacattatc
     2101 tgatgtaaaa gactatcagt tgctcattga aacaggtcag gcagaaaaaa tcagtcctaa
     2161 ggcaagtggt atgctgacat ggcaactggc gatatcagag gtggacaatg aactttatct
     2221 tcgtctggtg gcaaatggct gtggagggtt gttctctaag gactggatct cactttcaaa
     2281 gattgcatct gttctggaaa cacaacctgc taccggcttc tcatccggag tattcaggcc
     2341 gctattcaaa ggggtaagcg ccaacaatgc aggttttctg gctgctgttt tacgctcccc
     2401 ggatatatgt ctgctggagg cacatccaga gaagttattt actcatgtat tatacccgga
     2461 ctggagggag cggttgaata aacttaatac gatcagtagc caggaaaaga ttacagagga
     2521 ttaatttatt tcctgcactg ttatacaacc agaaatcacc acacaccact atttactttc
     2581 taaaggaaaa tttattatgc aaacccaata tgccttaaaa caggcaggat taactctgcc
     2641 tgttactaaa gaatttcagc agcatattac tactcttctg gcaaatcagg ttttacaaaa
     2701 aataactgag gcagtggtta ttaatttccg tgatccggac tacagtacag aatccggggg
     2761 ctttcatccc gttgaaatac ggtttatccg taagaataat gaatggtatt ttgattatgt
     2821 cacagatttt agttatatgg ggcgtgtcta ccctgagctg gaaaaagaaa ttgatttttg
     2881 ctggtcgggg aattatgtct tccattatct gattggggat atttcactgg cagcagagag
     2941 gaatgaactc tggtccctgt gggagcgtaa ttttatggag tatctctcta tggggattta
     3001 ccgggtaacg gtaactgtgg aaagttgttg acccgttatg aacaggaaga aagcgggcgg
     3061 ctttgtaatc tgctgcatgt ggcgtgcatc acagcatgga gatgatgtct ctttttcctg
     3121 gctgttcgca taaacaagtg cagacacggg ggatctcaga tccctcgtgg tttttgcccc
     3181 ctatggagca ttctcctgta gcagggtgat aacggttaat ccattacccc aacgacagta
     3241 ttacaccggt aataaatgtt catgaataca gtcagttatg tgggttttga ggtggactat
     3301 gtcatcgttg acgataactg aaggttttac agatgtataa aactcgcggt gaaaagatcg
     3361 cagaaaggct gtgctggata ttacttcaac tgtataccgg taatacggta tcggttaatg
     3421 atatatgtca gcgtttcgcg gtcagtcgcc gaaccggaca gcgtgatttg ttgagagttg
     3481 aaactgtaac ggaaactatt tcacctggaa gggtaaggct ggcaaatttg ataagggaac
     3541 gagttagacg aaatttttaa tcgaatccat atcaattcta taaaatatga aacttaacgg
     3601 gagtaaatat acactcccgt tataatgcaa ccttttttta actactcaaa tgatattaat
     3661 ttaggtgcga cagtgttttt atgacatgtt aaattcgcaa gatcaataat atcattaaac
     3721 aattttatgt cgtcgtcagt gagctctacg tctggatcag ctaaatcggc acaggtaaca
     3781 tttaaccagt gtgttaatga atcaatcctg tttgaagtaa tatattcagt gtgaccaata
     3841 tcccacttcc agttgttcac ccgagcaggt ctgaatctca cctcccgaac cccaattggc
     3901 gttactgact ctacgagagg gtatatcgtg ttaagtactt tatcatgttc actgtagata
     3961 tttaaatgcc ccatttttga accgtaagcc attttcatta tgttctctgt ataacttgtt
     4021 attggggtgg ctccggccat agagattgtt aagagaatat tatcattttt aatatagggt
     4081 aatgtattgt ttattaatcg agtccctaaa gaatgaccca ttatgatcac attacgcttt
     4141 tctttaacgt atatctcgtt tattacgtgt gctaattttt ctctgttatt aactgctgct
     4201 ttcgttgcat tataccaggc ctgacgcgct ttaaatggaa tggagattga tgacaataaa
     4261 ggagatgttc tcagggggaa gagagtatat aacccccact tgttcgctat gtcagaactt
     4321 aatatggata gtagatttgc cgattcccat ttatagtatc tgacatcaag gccacaccct
     4381 gctttaattc ggttaaacca tgatatatca ttttcggtca gaaaccctga tacaacaaga
     4441 agaaccgggc attttttcat ggtaactcca ttttttagct tacaaaaata gcactcaatg
     4501 aaaattatat tatccgtgtc attacggggc gttacttggc tgggtgtaac gtttcagtgc
     4561 ggattgtata tcttcagcaa gttgttgacg gattgaggcc ggttgtatta cttcgacatc
     4621 cggtatccag tatttcatta aaggcattat ctgatctgtg tgtgttacac gtgatatcac
     4681 cagtaaatcg ccagaccggg aagtatgaat tatttcctgt cctggcaata gatgacggtg
     4741 ggtaaaccac ggagcaacat gtgcggaaac tgaaataaga acttcgataa ggtcttcgct
     4801 ataccagata ccatctgtct tttctatttt ttcattaatt tcggggatta tcctgaaaga
     4861 gatattactc atgcaaacgt ctttgacaga tgagataaca aagcttttta atgtactgtc
     4921 atgggcggca gcgagatacc acaatccgtt gtcattaacc agtctgtatg gctcaacagc
     4981 ccgaaactct ttatctttgt aattaaaatt tactttcttc tgtgacagga tggcatcgga
     5041 taatacattc agaattgaca tgaatgatcc gcagttttcg tatggacgtt gttttatcag
     5101 aaacgggttg tttttcgtgt tacctaacaa aatactcagt agtctgtcat cccagcgagg
     5161 gaacagccct tcaataccca ggatgcgcgt gaaatgacgt aaatcagcat tgctgcgttg
     5221 accgagaaca ccgtcagaca ggcgatagcg accattttgt cgaatcagat taagatagga
     5281 caggcgctga gtaagatcac gacggatagt tttttcgcaa acgttaaatt cctcagataa
     5341 ctcccggacg gctagtgact caccattgtt caggcgagtt aatatcaatc cgagtcgata
     5401 tccaagttta tcgtgcttac ccattcgttt atccgattaa tgccagcgaa gaggatattt
     5461 tcagcaaagt aacggacagg atgagtccac ttttactgtg acggagaaaa aaattaactc
     5521 attaattatc aatatattaa aacattgctg gaatcatcga ataacatgtg gactcatcct
     5581 gtccatgtgt atttttacgt tcagggtgat aattattatc ctgacctgta catgacctgt
     5641 ttttgctgca tatttgacca tgcacttttg ccatcaagtc gga
//
LOCUS       DAPHJE010000199         5682 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPHJE010000199 DAPHJE010000000
VERSION     DAPHJE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.201"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..881
                     /locus_tag="RH222_004270"
     CDS             171..881
                     /locus_tag="RH222_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3799191.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            923..3568
                     /locus_tag="RH222_004271"
     CDS             923..3568
                     /locus_tag="RH222_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3799192.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSATNGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVKAPGADNVKIENQTGI
                     HTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQIG
                     GKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDKNS
                     NCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3582..4112
                     /locus_tag="RH222_004272"
     CDS             3582..4112
                     /locus_tag="RH222_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3799193.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4125..4628
                     /locus_tag="RH222_004273"
     CDS             4125..4628
                     /locus_tag="RH222_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3799194.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4688..5602
                     /locus_tag="RH222_004274"
     CDS             4688..5602
                     /locus_tag="RH222_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799195.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1640 a         1229 c         1261 g         1552 t
ORIGIN      
        1 taaaatattt actttgtagc aaggttttcc tattaaaaga gaaccaacat ttattacaaa
       61 cgcattaagt ctggaaaccc ggcgactcgc caatgtatta gcaacattaa tcgattgtct
      121 gatgtaacgg ttttcccgct tatcctctta acacatggag aatgcatcag atgcaaacta
      181 cgcgtacccc ctactctatt tcctttatgg cgacagtgtt acttttattg ctctttgctt
      241 gccatagcac cgttgccaac gcagctgtcg cactgggtgc gacgcgggta atttatccgg
      301 caaaccagaa acaggtttta ttgcctgtca cgaataatga tcctgccagt gtctatctca
      361 ttcagtcatg gatagaaaat gcaggtgacc aaaaagacac ccagtttgtc attaccccac
      421 cgcttttttc catgcaaggt aaaaaagaga atacgctgcg gataatcaac gctactaatc
      481 atcagttacc aggcgaccgt gaaagcctgt tctgggtgaa tgttaaagct attcctgcta
      541 tggaaaaaga ccagaagaat gagaatacat tacagctggc aattattagc cgcataaaaa
      601 tgttttatcg ccccacgaac ctggcaatgg cacctgaaga agcgcctgca atgcttcggt
      661 ttcgtcgcag cggtagcaaa ctcacgctta ttaatccaac gccctatttc attaccgtaa
      721 caaatatgaa agcaggaaat agcaatctcc caaacaccat ggtgccgcca aaaggtgaag
      781 tatcggtgga tatcacgcat gctgcaaccg gggatatcag ttttcagacg atcaatgatt
      841 acggtgcatt aactccgcgt attaaggcaa ccatgcagta agccagtgac ctaattaata
      901 ataaactcaa tcggaattaa taatgacagc cattcgtgcc gcatttaagg catatcgtat
      961 gcatcaggtg ttaataatgc ctcgctttgc acggcttacc attgcccttg gcttagcaac
     1021 tgcggtattt cccgttgacg cagagtatta ttttaatcca cggtttttaa gcaatgatct
     1081 tgctgaatct gtcgatttat ccgcctttac taagggacgc gaggcaccac ctggtacata
     1141 tcgggtcgac atttatttga acgatgaatt tatgactagt cgggatatta cttttattgc
     1201 agatgataat aatgcagaac tcatcccatg cctgagtaca gatcttcttg tcagtcttgg
     1261 tataaaaaaa tcagcgttat tagacaataa agaacattcg gcagaaaaac atgtgccgga
     1321 caacagcgca tgcacaccac ttcaggatcg tttggccgac gcatcaactg agtttgatgt
     1381 tggtcagcaa catttgtcac tctccgttcc gcaaatttat gttggcagaa tggctcgcgg
     1441 ctatgtttct cctgatctgt gggaagaagg aataaatgct gggcttctaa actatagttt
     1501 taatggtaat tctattaata atcgtagtaa ccataatgca ggaaaatcaa actatgcata
     1561 tttgaattta cagagtggca tcaacattgg tagttggcga ctacgcgata actcaacgtg
     1621 gagttataac agtgggagta gcaattcatc tgacagcaat aaatggcagc atatcaatac
     1681 gtcggctgaa cgtgacatta ttcccttacg ctcacggtta acggtaggtg atagttatac
     1741 cgatggcgat atttttgata gtgtgaactt tcgtggcctc aaaataaatt caacagaagc
     1801 gatgttgccc gatagccaac atggtttcgc tcctgtgatt catggtattg cccgtggaac
     1861 cgcacaagtg agtgtaaaac agaacggata cgatgtttat cagactactg ttccacccgg
     1921 cccttttact attgatgata tcaactctgc gaccaatggt ggtgatctgc aagtaaccat
     1981 aaaagaggca gacggcagta ttcagacatt atatgttcct tattcgtctg ttccggttct
     2041 ccaacgtgct ggatatacgc gttatgcgct tgccatgggg gaatatcgta gtggaaataa
     2101 cctgcaaagc tctcccaagt tcatacaagg tagcttgatg catggtctgg aaggaaactg
     2161 gacaccttat ggcggaatgc aaattgcaga agattatcag gccttcaacc ttggtattgg
     2221 gaaagattta ggactttttg gtgccttttc tttcgatatc acgcaggcca atacgacact
     2281 tgcagatggc acccgtcaca gtgggcaatc ggttaaatcc gtctacagca aatccttcta
     2341 ccagacagga accaatatcc aggtcgcagg atatcgctat tctacgcaag gtttttataa
     2401 cttatccgac agtgcctaca gtcgaatgag tggttacacc gtcaagcctc ctaccggaga
     2461 caccaatgag cagacacaat ttattgatta ttttaatctg ttctacagta agcgtggtca
     2521 ggaacaaata agcatctctc agcagcttgg aaattacggt acgacatttt tcagtgccag
     2581 tcgccaaagt tactggaaca cgtcacgcag cgaccagcaa atatcatttg gattaaatgt
     2641 gccgtttggt gatattacga cttcgctgaa ttacagctat tccaataata tatggcaaaa
     2701 cgatcgggat catttactcg cttttacgct taatgttccc ttcagtcatt ggatgcgtac
     2761 agacagtcag tcggcatttc gtaattcaaa cgccagttac agtatgtcaa acgatttgaa
     2821 aggcggcatg accaatctat cgggggttta tggcactctg ctgccggata ataacctgaa
     2881 ttatagcgtt caggtcggta acacccacgg aggtaataca tcgtctggca ccagtggtta
     2941 cagttctctt aattatcgtg gagcttacgg taatacgaat gtcggttaca gtcggaatgg
     3001 tgacagcagc cagatttatt acggaatgag tggtgggatt attgctcatg ctgatggcat
     3061 cacctttgga cagccgctgg gcgacacaat ggttaaggct cctggcgctg ataatgtcaa
     3121 aatagagaac cagaccggaa ttcataccga ctggcgtggc tatgcaatat taccatttgc
     3181 gacagaatat agagaaaatc gtgtcgctct taacgcgaat tcccttgcag ataatgttga
     3241 actggatgaa accgtagtca ctgtcatccc aactcacggt gctattgcca gagcaacatt
     3301 taatgcacaa atcggcggga aagtattaat gacgttgaag tacggtaata aaagcgttcc
     3361 attcggtgca attgtcactc acggagagaa taaaaatggc agcattgtcg cggaaaatgg
     3421 tcaggtttat ctgactggac ttccacagtc agggaaatta caggtttcat ggggcaatga
     3481 taaaaactca aactgtattg tcgattacaa gcttcctgaa gtctctcctg gaaccttgct
     3541 gaaccagcag acagcaatct gtcgctaagg aacaatagat tatgaaatac aataacgtta
     3601 ttttcctcgg tttatgtctg gggttaacca cctattctgc tttatccgca gatagcgtta
     3661 ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt ctcatcggat tcgcttaatt
     3721 ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc aacgaccggt agcacaagtc
     3781 cagccgtccc ttttcagatt acgttaagtg aatgcagcaa agggacaacg ggggttcggg
     3841 ttgcatttaa cggtattgag gatgcagaaa ataatactct gttgaaactg gatgagggaa
     3901 gcaatacggc ctccggttta ggtatagaaa tactggacgg aaatatgcgt ccggtgaaac
     3961 tgaatgacct tcatgccggg atgcagtgga tcccactggt accagaacag aacaatattt
     4021 tgccttactc cgctcgtctg aagtcaactc agaagtccgt caatccggga ctggtgaggg
     4081 cttcggcaac ctttaccctt gaatttcaat aatcagagtc agatatgaaa agacttcaca
     4141 agaggttcct gttagctacg ttttgcgcgt tattcacagc aactctccag gccgccgatg
     4201 tcactatcac tgttaatggt cgggtagtcg ctaaaccctg cactattcaa accaaagaag
     4261 ctaacgttaa tctcggggat ctttatacgc gcaatctgca acaacctggt tctgcatctg
     4321 gctggcacaa tattactttg tcattaaccg attgtccggt tgaaacaagt gcagtgacgg
     4381 caatcgtgac aggttcaact gacaatacgg gttattacaa aaatgaaggt actgccgaaa
     4441 atattcagat agagctgagg gatgaccagg atgcgacgtt aaaaaatggc gatagcaaaa
     4501 cggttattgt tgatgagatc actcgtaatg cacagtttcc acttaaggca agagctatca
     4561 cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct aatcaatgtg atctacacct
     4621 ggcaataacg tcgattcgat tttcttagcc ttctttttaa atcaattttt taaccttagg
     4681 atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat
     4741 ggcaggtaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagctgg
     4801 tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt
     4861 agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca
     4921 tataaacctg gtacaaggct cagctttcgc aggctctcta cagtcatata aaggatcgct
     4981 gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat
     5041 tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag tcggggcggc
     5101 cggtggagtt gtcataaaag ccggggaagt tattgcccgt attcatatgt ataaaattgc
     5161 aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacagtgt
     5221 tgttatgcca acaggcggct gcactgttga tagtcgtaat gtcaccgtgg atctcccgga
     5281 ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc
     5341 attttatctt tcgggtgcca ccactgacag tgcccgacaa gtgtttgcta acacagcacc
     5401 tgatgcaaca aaagcatcgg gagtgggtgt ctctctgatg cgtaatggaa aaatactggc
     5461 tacgggagaa aatgtctccc tgggaacagt aaataaaagc aaagtacctc tgggtttaag
     5521 cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt cagtcattgg
     5581 tgtcactttt atctatgaat aattgtgatt ttgatcttag tctatgcgct ccattcacga
     5641 aaattgcgcg gataattatt ttgtaaaggc tattatccta ca
//
LOCUS       DAPHJE010000200         5676 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPHJE010000200 DAPHJE010000000
VERSION     DAPHJE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.202"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..782
                     /locus_tag="RH222_004275"
     CDS             42..782
                     /locus_tag="RH222_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3799196.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            817..3416
                     /gene="htrE"
                     /locus_tag="RH222_004276"
                     /pseudo
     CDS             817..3416
                     /gene="htrE"
                     /locus_tag="RH222_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
     gene            3433..4002
                     /locus_tag="RH222_004277"
     CDS             3433..4002
                     /locus_tag="RH222_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799197.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4017..4619
                     /gene="yadL"
                     /locus_tag="RH222_004278"
     CDS             4017..4619
                     /gene="yadL"
                     /locus_tag="RH222_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDV3799198.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4646..5242
                     /locus_tag="RH222_004279"
     CDS             4646..5242
                     /locus_tag="RH222_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799199.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5295..>5676
                     /locus_tag="RH222_004280"
     CDS             5295..>5676
                     /locus_tag="RH222_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV3799200.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKL"
BASE COUNT         1679 a         1164 c         1265 g         1568 t
ORIGIN      
        1 atattattcc ctgttttaat taactctgtc agggatggtt tatgtttttt aacaccaaac
       61 atacaacagc tttatgcttc gtaacctgta tggcttttag ttcatcgtct attgcggaca
      121 ttgtcatttc gggtactcgc gtaatatata aaagcgatca aaaaagtgtc aacgtacgtc
      181 tggaaaataa agggaataac ccgttgcttg tccagagttg gttagatact ggcgatgaca
      241 acgctgagcc tggcagtatt acagtccctt ttactgccac gccgccagta tcgcgtattg
      301 atgccaaacg tgggcaaaca atcaaattaa tgtacacagc cagcacctca ctgcctaaag
      361 acagagagag cgtgttctgg tttaacgtac tggaagttcc accaaaacca gatgcagaaa
      421 aggtcgcgaa tcaaagtctg ctgcaactgg catttcgcac acgtataaaa cttttctatc
      481 gcccggatgg attgaaggga aatccctctg aagccccgtt agccctgaag tggttctggt
      541 caggttcaga aggtaaggcg tcattgcgag tgaccaatcc aaccccttac tacgtctctt
      601 ttagcagtgg tgatttagaa gctagcggta aacgctatcc gattgatgtg aaaatgattg
      661 caccatttag tgatgaggtc atgaaagtca ctggccttaa tggcaaagca agttctgcaa
      721 aagtacattt ttacgccatt aatgattttg gtggcgccat tgaaggtaat gccagtctgt
      781 aatcaggcaa ggatataatt ccgcaggaag cataacgtga ctataaaacc aactaaaaat
      841 tatcataatc atctgacccg tatcgctacg ttttgcgcgc tgctgtattg caatagtgct
      901 ttctgtgctg aacttgttga atatgaccat accttcctga tggggcagaa tgcctcaaat
      961 attgatctca gccggtatag tgaaggtaac cctgctatac cgggtatgta cgacgtcagt
     1021 gtttatgtca acgatcaacc tattattaac caaagcatta catttattga aattgaagga
     1081 aaaaaaacgc acaagcctgt atcacactta agaatttatt gcagtttcat ataaattctc
     1141 ccgatataaa taacgaaaaa gccgttctgc ttgccaggga tgaaacactc ggcaattgcc
     1201 ttaatttgac ggaaattatc cctcaggctt ctgttcgtta tgacgttaac gagcaacgtc
     1261 tggatataga cgttcctcag gcatgggtaa tgaaaaatta ccaaaactat gttgatccat
     1321 cgttatggga aaatggcatt aatgcggcca tgttgtcata caacctcaac ggatatcata
     1381 gtgaaactcc tggtcgaaga aatgacagca tttatgctgc ttttaacggt gggatgaatt
     1441 taggtgcatg gcgactgcgt gcctcaggca actacaactg gatgaccgat tctggcagta
     1501 attatgattt taagaatcgg tatatacagc gtgatatcgc ctcgctgcgt tctcaactca
     1561 ttcttggtga gtcttataca acgggcgaaa cctttgattc tgtcagtatc cgcggcattc
     1621 gtttatacag cgacagccgc atgttgcctc cgacattagc gagttttgcg cctatcattc
     1681 atggcgttgc taataccaac gccaaagtca ctattacgca aggtggctat aagatttatg
     1741 aaacgactgt gccgccaggc gctttcgtca ttgatgatct gagtccgtca ggttacggca
     1801 gcgatcttat tgttactgtc gaagaatccg atggctcaaa gcggacattc tcgcaacctt
     1861 tttcatcggt cgttcaaatg ttacgccctg gcgttggacg ttgggatatt agcggcggcc
     1921 aggtcttaaa agatgatatt caggatgagc ctaatttatt tcaggccagc tactactacg
     1981 gtctgaataa ctatctaacg ggttatactg gtattcagat taccgataat aactataccg
     2041 ctggtttgtt aggtcttggt ctgaatactt cagtcggtgc attttctttc gatgtgactc
     2101 actccaatgt tcgtataccg gatgataaaa catatcaggg acaaagttat cgtgtttcct
     2161 ggaacaagtt attcgaagaa acaagtactt cactgaatat cgcggcctat cgctattcga
     2221 cacagaatta ccttggtctt aatgatgcac taactctaat tgatgaagtg aaacatcccg
     2281 aacaagatct tgaaccgaaa tccatgcgta attactcgcg catgaaaaat caggttacgg
     2341 tcagtattaa ccaaccgcta aaatttgaga aaaaagatta cggttcattt tatctttccg
     2401 gaagttggtc cgattactgg gcttccggac aaaatcgtag caattactct attggctaca
     2461 gtaacagtgc atcctggggc agctacagtg tcagtgccca gcgttcatgg aatgaagacg
     2521 gcgacactga cgatagcgtt tatctcagtt tcaccattcc tattgaaaaa ttacttggca
     2581 ctgaacaacg gacttcaggt ttccagagta ttgatactca aatgagcagt gactttaagg
     2641 gtaataacca actcaacgtc agtagcagtg gctatagtga taacgctcgc ctcagttata
     2701 gcgtaaatac tggctatacg atgaataaag ccagcaaaga tttgagttat gttgggggtt
     2761 atgccagcta tgaatcaccg tggggaaccc tggcaggttc agtttctgca aatagcgata
     2821 acagtcgtca agtgtctctc agcaccgacg gtggttttgt attgcatagc ggcggcctga
     2881 ccttcagtaa tgacagtttt agcgactccg atacactggc ggtagttcag gctccaggtg
     2941 ctcaaggagc gcgaataaat tatggcaaca gtactatcga tcgatggggt tatggtgtca
     3001 ccagcgctct ttctccttat catgaaaacc gtatcgcgct ggatatcaac gatcttgaga
     3061 acgatgttga attaaaaagt accagtgcag tagctgtacc gcgtcagggt tcagtcgtct
     3121 ttgctgattt tgaaaccgtg caagggcaat cagccattat gaacatcaca cgaagtgatg
     3181 gtaaaaatat tccatttgct gcagatattt atgatgagca aggcaatgtc attggtaatg
     3241 ttggacaggg tggacaagca tttgttcgtg gcattgagca gcagggaaat atcagcatta
     3301 aatggctcga agaaagtaaa cccgtaagtt gtcttgcgca ttatcaacaa agttcagaag
     3361 cagaaaaaat agcgcaatct attattctga atggaattcg gtgtcagatt gagtaactac
     3421 aagggacgtc aaatgataaa aataacgcca cataaaatag cgatactgat gggaatatta
     3481 ttatcaccat cagtatttgc aacggatatt aatgtagagt ttacagccac tgtcaaagcg
     3541 acaacctgta acatcacact tactggtaat aacgtcacga atgatggcaa taataactac
     3601 acattgagaa tccctaagat gggtctggat aagatcgcga ataaaacgac agaatctcag
     3661 gctgatttta aactggttgc tagtgggtgc agcagtggca tcagttggat tgataccact
     3721 ctgaccggaa atgcatcatc aagctcacct aagcttatta taccgcagtc tggtgattca
     3781 tcttcgacga caagtaatat cggtatgggt ttcaaaaaac ggactactga tgatgccact
     3841 ttccttaaac ctaacagtgc ggaaaagata cgctggagca cagacgagat gcagcccgat
     3901 aagggtcttg aaatgaccgt tgcgctacgt gaaacagatg cagggcaagg cgtaccgggg
     3961 aattttcgtg cactggcaac gtttaatttc atctatcaat aagaaggata attaagatga
     4021 catttaaaaa tttacgttat ggattatcca gcagtgtcgt tttggctgcc tcattgttca
     4081 gcgtaccctc ttatgcggca acagacagca ttgggctgac cgtgattact accgtagaaa
     4141 taggtacttg taccgctaca ttagtaaatg actctgatca ggacatttct gttgttgact
     4201 ttggcgatgt atatatttct gaaatcaatg ccaagaccaa agttaaaaca ttcaaactca
     4261 aattcaaaga ctgtgcgggt atccccaata aaaaagcaca aataaaatta accaagcgag
     4321 ccacatgcga aggtactgct aatgacggtg cggggttcgc aaatggatcc acagccgcag
     4381 ataaagcaag tgccgtcgcc gttgaagtct ggagcaccga aactccggca acaggtagcg
     4441 caacacaatt tagttgtgtg acaccagcat ctcaagaggt cactatctcc aatgcagcca
     4501 acgcggttgt ttattatccg atgagtgctc gactggtcgt cgaaaaaaat aaaaccgtaa
     4561 gcaatgtcac tgcgggtaaa ttttctgcac ctgccacatt tacagtaacc tataactaac
     4621 agactgacag atccaggaac tatatatgca tcccactcaa cgtaagctga tgaagagaat
     4681 aattctgttt ctgtcactac agtttcccat tacctatcca gccatcgctg ggcaggatat
     4741 tgaccttgtt gccaatgtaa aaaacagcac ttgcaaaagc ggaatcagta accagggtaa
     4801 tattgatctt ggcgtcgttg gggtgggata tttttcagat aatgttactc ctgaaagcta
     4861 tcaaccaggt ggaaaagagt tcactgtcac agtatcagac tgtgcattac agggaactgg
     4921 cgatgtgctt aatcagttac atattgattt tagagccctt agcggtgtca tggctgcagg
     4981 ttcaaggcaa atatttgcca atgaagtcgc aaccggggct aaaaatgtcg gtgtcgttct
     5041 cttttccatt caggacccga ccaatatatt caatgttatt agttcagcag gaaattctcg
     5101 ttccgtgtat ccagtaatga cttctgccct gcataattct tcatggaaat tttatgccag
     5161 aatgcaaaaa atcgatcccg cgctggatgt catatctggc caggtaatga gtcatatatt
     5221 agttgatgtg tactacgaat aatagatcaa aacaaataac tgcgactgtt acagcagtag
     5281 atggtggagt gataatgaag aaattcttca gacattttct ttttttaatt ctgtgcttga
     5341 gttgttatac cgcgagtgct ggcactgatg ataacgtagg atatattgtt gggaatagtt
     5401 acggcgttgg tccgtcagat caaaaatgga gagaaacggg gccgaatggc gatgctactg
     5461 tcatattcag atatgcaaca agtacaaata atttagtttt ctacaaaccg acgcagcttg
     5521 gacctacagg tgtaaaatta cagtggagtc agttagatac cgcttctggt ggtggttttc
     5581 tttattgcaa cagaagtgat agcacaagtg gtagcgcaat gcgtattgaa aatgcaatgg
     5641 ttgactcagg taaaatgtat ggctcccata aattat
//
LOCUS       DAPHJE010000201         5583 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPHJE010000201 DAPHJE010000000
VERSION     DAPHJE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.203"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(111..2180)
                     /gene="glyS"
                     /locus_tag="RH222_004281"
     CDS             complement(111..2180)
                     /gene="glyS"
                     /locus_tag="RH222_004281"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV3799201.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2190..3101)
                     /gene="glyQ"
                     /locus_tag="RH222_004282"
     CDS             complement(2190..3101)
                     /gene="glyQ"
                     /locus_tag="RH222_004282"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV3799202.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3196..3495)
                     /locus_tag="RH222_004283"
     CDS             complement(3196..3495)
                     /locus_tag="RH222_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDV3799203.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3670..4665
                     /gene="wecH"
                     /locus_tag="RH222_004284"
     CDS             3670..4665
                     /gene="wecH"
                     /locus_tag="RH222_004284"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV3799204.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4707..5144)
                     /locus_tag="RH222_004285"
     CDS             complement(4707..5144)
                     /locus_tag="RH222_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV3799205.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5190..5531)
                     /locus_tag="RH222_004286"
     CDS             complement(5190..5531)
                     /locus_tag="RH222_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799206.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1362 a         1435 c         1370 g         1416 t
ORIGIN      
        1 ttaatatatg agataatctt tattcagcag aagattatta aaggttgctg tattatttag
       61 cgataataaa aaaagcctgc cagatggcag gctttttaat aacggcgtta ttactgcaac
      121 agcgaaatat ccgcaacgcg caggaacagt tcgcgcagtt tctccagcat ggtcagacgg
      181 ttgagacgca attctttgtc atcaaccatg accatcactt tatcgaagaa agcatcaacc
      241 ggctcacgca gctctgccag ttcgaccagc gcatcctggt aacgaccttc cgcaaagtac
      301 ggctccagct tgtcacgtag caccacaacc tgcatcgcca gtttaatttc ttccggctct
      361 ttcagggtag aggcattcac gcggtcgctc agcacttcgt cagatttcgc caaaatgttg
      421 gagacacgct tgttcgccgc cgccagtgca gcagctgctt caagggtacg gaagtgcgat
      481 accgctttca tgcgggcatc gaaatcagcc ggacgagtcg gacgacgcgc cagtaccgcc
      541 tggatggtgt caacggtgta accttcgtcc tgataccagg cgcggaagcg accgagcata
      601 aagtcgataa catcatcaac cacgttggcg ttagtcagct tatcgccata cagacgcact
      661 gcttcttcgg tcagcgtttg cagatcaagg ttgaggttct tctcaacgat aattcgcagc
      721 acgccaagtg cggcacgacg cagcgcaaac gggtctttgt cgcctttcgg atgctggccg
      781 ataccgaaga tacccgccag agtatccatc ttgtcagcaa tcgccagcgc acaggctacc
      841 gggttagacg gcaggtcatc accggcaaag cgcggctgat actgctcatt cagcgccacc
      901 gcgacatctt ccgcttcgcc atcgtgacgc gcatagtgca tccccataac gccctgggtg
      961 tcggtgaact cgaagaccat gttggtcatc aggtcacact tagacagcag gcccgcacgg
     1021 gttgcgtggt taacgtcagc gccaatctgt tcagcaatcc agccagccag cgcctggatg
     1081 cggtcagttt tgtcacgcag cgtacccagc tgttgctgga acagcacggt ttgcaggcgc
     1141 ggcaggttat cttccagacg ttttttacgg tcggtgttga agaagaactc ggcatccgcc
     1201 agacgcggac gaacgacttt ctcgttaccg gagataatct gctgcggatc tttcgattcg
     1261 atgttggcaa cgaagataaa gttcggcagc agtttgccgt cgttcgcata caccgggaag
     1321 tatttctggt cacctttcat ggtgtaaacc agcgcttcag ccggcaccgc gaggaatttc
     1381 tcttcgaatt ttgcggtcag aacgaccggc cactccacca gcgaagccac ttcttccagc
     1441 aggctttcgc ttaagtcagc gttaccgcca atcttacgcg ctgcttcttc ggcatcggct
     1501 ttaatcttcg ctttacgttc ttcgtaatcg gcgatgactt tcccgcgctc acgcagaatt
     1561 tccggatact gatcggcgtt gtcgatggtg aattccggtt cgcccataaa gcggtggccg
     1621 cgaatcacgc gatcggactg aatgcccaga atggttgccg gaatgacttt gtcgcccagc
     1681 agcagggtca cggtgtgcac cggacgcacg aagtgcacgt cgcttgcgcc ccaacgcatc
     1741 agtttcggga tcggcagttt cgccagagaa gtcgcaacca tattcggcag cagtgcttcg
     1801 gtgctttcgc ccttcacatg ggcgcgatac agcagccatt cgcctttatc ggtagtcaga
     1861 cgctcagcct ggtcaacggt aataccgcaa ccacgcgccc aaccttctgc cgctttgctc
     1921 ggtttgcctt cagcgtcgaa cgcctgggca atcgccgggc cgcgtttttc gatttcacga
     1981 tccggttgcg cttccgccag gttagctact ttcagcgcca gacgacgcgg agcagcaaac
     2041 cattgaacgg tgccgtgtgc gaggccagcg ttatccagct ccgcagtaaa attcgcagca
     2101 aaggactcag ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc gatttccacc
     2161 agaaaagttt tctcagacat agccgcctct tacttatctt tgttgcacat cgggaagccg
     2221 agggcttcac gggaagcgta gtatgcttct gccactgctt tggtcagggt gcgaatgcgc
     2281 agaatgtagc gctgacgctc ggtgacggag atggctttac gcgcatccag caggttgaag
     2341 ctgtgggcgg ctttcagaat acgctcgtag gctggcagcg gcagcggatt ttccagcgcc
     2401 agcagctgct gcgcttcttt ctcgtactgc tcaaagcagg tgaacaggaa gtccacatcc
     2461 gcgtattcga agttgtaagt ggactgctcc acttcgttct gatggaacac gtcgccgtag
     2521 gtggttttac ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc tacgccctga
     2581 atgtacatgg ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg tttacactcc
     2641 agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt tagccacact
     2701 tcccagccca gtccccaggc acccagagtc gggttttccc agttatcttc cacgaaacgg
     2761 atgtcgtgaa tagtcgggtc catgcccagc tctttcagag aaccgaggta cagctcctga
     2821 atattgtccg gcgatggctt aatgaccacc tgaaactgat agtagtgctg taaacggttg
     2881 gggttttcgc cgtagcgacc atcggtcggg cgacgagaag gctgaacata agcagccgcc
     2941 atcggttctg gccccagcgc gcgcagacag gtcattgggt gagaggttcc cgcgccgact
     3001 tccatgtcca atggttgaac aatggtgcag ccctggcgag cccagtaatc ctgtaaggtc
     3061 aggatcaagc cctggaaggt cctggtatca aacttttgca tattatttcg tgctggatac
     3121 gtgtggattt aaaggaaggg atcagtatac ccgctggatg gaagatatac agtacgaaac
     3181 gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa tggccatctg cgtcaaaaga
     3241 acagacaaac tgctctttac ggaaggtata accgcgcatt tcatagcttc cctggaactg
     3301 ttcgaaggcg gtgacgtcga ttttttgctc gccggtgtta taacgttttg ccgcctgatc
     3361 cttgcataac gcttccatat tcaacgaacg cagaggatca acctttaccc gttgtgcttt
     3421 ctgcacgggt gacggtgtag aacaacccac tagcaccata agcgccattg ccggaaagaa
     3481 agcgttcatc atcattaaat taccgtctgg tttgcaggtg agtcttatta tttatatcgg
     3541 tatagtggct gatatcttac gaatttcctt aagcctctat aagctgtagt gataaaactc
     3601 agattattcc agtgcgccaa ctgaatgatt tatcggtcag acttttttta accagataca
     3661 gagacacaga tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta
     3721 atggtggtga tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc
     3781 gtcacctggg atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt
     3841 ttcatgatat ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg
     3901 cgtatcggct tatgcctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt
     3961 acctccatca atatggagtt agcgctgaaa aacctgctgc aaaagccagt gttttaccac
     4021 ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag
     4081 aacgtcggcg gaaaaatgtt gctggtgcta atggtggtga ttggcattat cgctaaccca
     4141 aacacagtgc cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat
     4201 ggcgatactt tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac
     4261 acacagcata aagcactgtc gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt
     4321 atctctcgcg ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat
     4381 ctttactgtg ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac
     4441 acgctggata cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata
     4501 tacggattcc acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat
     4561 tggccaatac tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt
     4621 tctatgctgg tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca
     4681 cattggactg accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt
     4741 tcttttggcg ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac
     4801 aagcttaaga agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc
     4861 cacagaccta ccatcagtaa tgcaacagag ataataaaga cagtcaggca ggtcatataa
     4921 taaatggaag tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca
     4981 gcagaaaaca gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc
     5041 tctgcattcc atagccctaa cagataggta acgataccac caacgagagc tatccatgac
     5101 acaatactaa aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca
     5161 ttcctgaatg tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca
     5221 aacaagcaga caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca
     5281 tttaaaacac cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg
     5341 gtaaaaaaat tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa
     5401 gttgctgtca ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc
     5461 catagcccaa ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt
     5521 gaggttttca taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga
     5581 caa
//
LOCUS       DAPHJE010000202         5457 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPHJE010000202 DAPHJE010000000
VERSION     DAPHJE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.204"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..108
                     /locus_tag="RH222_004287"
     CDS             <1..108
                     /locus_tag="RH222_004287"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV3799207.1"
                     /translation="LRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            180..1538
                     /gene="mltD"
                     /locus_tag="RH222_004288"
     CDS             180..1538
                     /gene="mltD"
                     /locus_tag="RH222_004288"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV3799208.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1586..2356)
                     /locus_tag="RH222_004289"
     CDS             complement(1586..2356)
                     /locus_tag="RH222_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3799209.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDISHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
     gene            complement(2434..3234)
                     /locus_tag="RH222_004290"
     CDS             complement(2434..3234)
                     /locus_tag="RH222_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV3799210.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3475..4389
                     /locus_tag="RH222_004291"
     CDS             3475..4389
                     /locus_tag="RH222_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3799211.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4386..5189)
                     /gene="dkgB"
                     /locus_tag="RH222_004292"
     CDS             complement(4386..5189)
                     /gene="dkgB"
                     /locus_tag="RH222_004292"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV3799212.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5352..5428)
                     /locus_tag="RH222_004293"
     tRNA            complement(5352..5428)
                     /locus_tag="RH222_004293"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5392..5394),aa:Asp,seq:gtc)
BASE COUNT         1446 a         1316 c         1348 g         1347 t
ORIGIN      
        1 ttaagaacag aagatattga tttaattaat gtaattaatg aagaaacatt attgcaacaa
       61 cctgaagaac gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt
      121 gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta agcaactatt gacacacaca
      181 tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggctgccaa agtaccggca
      241 acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta
      301 caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg
      361 ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga
      421 aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt
      481 atatgtactg gatagccggg caagttaaaa aacgtaacat gcctatggaa ctggtactac
      541 tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gccgcaggca
      601 tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg
      661 acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaacatgatg cagcgtctga
      721 acaagatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc
      781 gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt
      841 taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc
      901 tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg
      961 cgcgtgtgca cctgagcagc ccggttgaaa tggcgaaggt tgcagatatg gcggggattt
     1021 ccgtcagcaa gctgaagaca ttcaacgctg gcgtaaaagg ctccacgctg ggcgcaagtg
     1081 gcccgcagta cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctggctt
     1141 caggcgaaat tgctgctgta cagtcgacgc tggttgccga caatacgccg cttaacagcc
     1201 gtgtttacac cgtacgctct ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtaa
     1261 gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa
     1321 gtttgacgat tggtgcaggc agtagcgcac agcgactggc aaacaacagc gatagcatta
     1381 cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca
     1441 tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc gataagctga
     1501 cgttgtttgt gaaaaacaac aacatgccag attcctgaca aaccagataa taaaaaggca
     1561 ccgattcccc cggtgccttt tttatttatg ccgctttatg tgcatctacc atgatgatat
     1621 cactggtgaa aaagccatca ttctgcaagg caaaatacgt tctcacctct gtcgatgcgc
     1681 tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcgcgaccc
     1741 atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt
     1801 cattgattaa cgtcaaccac tcaccgctgg cgtagtttcg tacgtgagag atatcgcgta
     1861 atgcttctac cgtctgtaac cagatgtcgc gcactgggtg acccggagac attacgtcca
     1921 tcacaatcag cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa
     1981 catcatgcca gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa
     2041 atggcagact ttcggcatat ccctggcggg tggtgatatt tttcagttgc cgggcttcgg
     2101 cagcttgtgc cacgacatcc agcatatggg cagataagtc atacgccacc accgtgctca
     2161 cgttttgcgc agcgacaaag ctggcatgcc ctgctccgca gcccatatca agtacacttg
     2221 caccaggata atcagccaga cgcaccgcca ggcgctgtaa atctcgtccg gatgcatgta
     2281 cggtactggt cagatattcg caggcttgag agctaaactg cttttctacg tggtcatggt
     2341 gggattgtgt tgtcattgcg ctgtcctttt gttgtttgag tataaaagga cagcgccaaa
     2401 cgttggtctg ccctgtggca gacctgacat accttattta tcaggcttgc cgggactgaa
     2461 ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc aatacagaag cttcgctgac
     2521 gttcagacca cggtagaaaa caaaatcgag cgggcggcca aacgcccgac ggcgctgatc
     2581 atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca aagcgatata acgcgttcat
     2641 ccttctacgg ctccaggcat tgaaatctcc cgccataatg accgggccgc tgtggtgagc
     2701 tatctgatca ccaataggaa gtaactgctt actatagaca tccacgccca gactgaagtt
     2761 gacggcgtgt atattaacca ccatcaacag gcgggtgtca ggtaatggat agaccgtcac
     2821 cagtgccgac ttcgccagac gcaaaatggg ttctcgttca cgtaacgggc agcaatacac
     2881 tggatgcgcc gccgaaaggg tcattacacc ggaaggatgt tgtggcagca cgaaagcggg
     2941 cacctgatcg gcggcaagat agttagcggt cgcaaactgt actaactctg gcgttgtctg
     3001 cgcttcctgc aataacacca gatgtgcatc tttgccatag ttctttaata ccgacaacca
     3061 ttcagcgcgt tgctgtttgt atatgttcca caccaggatc cgaatacgct cttcggtact
     3121 taacggttct ccaggtggta atgcctggcc gatgctcgca aaagaccccg gcggtaagat
     3181 cctttccgca ggttgtccgg caacatagcg catggcatag gtgttttttc gcacttttga
     3241 ctttgaaacc tctattacgt caacccttcc gcactcggga gggttctact gttataggga
     3301 ttttagctca ctctttcaac gagcaattgt atattgttat gtaagcaagt gcttacaccg
     3361 gtcagaattt tgctcattat ggagaacatt aaatcgcatg acatatcacg catccagatg
     3421 gtttaatgac aaataaattc aaaatatctc ttctattttg caacaggagc aaaaatgaaa
     3481 gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg aaagcggcag ctttagccgg
     3541 gcagcggaac aattagggca agcaaactca gcggtaagcc gggcggtgaa aaagctggag
     3601 atgaaacttg gcgttagcct gcttaatcgg accacgcgac aacttagcct gacggaagaa
     3661 ggcgagcgtt attttcgtcg cgtacagtca attttgcagg agatggcatc ggcagaatca
     3721 gaaattatgg agacgcgtaa tacaccgcgt ggactgttac ggatcgatgc cgcaactcca
     3781 gtggtgctgc actttctgat gccgttaatt aagcctttcc gtgaacgcta tccggaagtc
     3841 actttgtcgc tagtctcctc cgaaacgatt attaatttga tcgaaagaaa agtggatgtc
     3901 gcgatacgcg ctggtacgtt aacggattcc agcttacgtg ccaggccgtt atttaacagt
     3961 tatcgaaaaa ttatcgcctc ccccgattat atttcccgct acgggaagcc agaaacgatc
     4021 gacgatttaa agcaacatat ttgcctggga ttcactgaac ccgcttccct caatacctgg
     4081 ccgatagccc gtagcgatgg acaattacat gaggtgaagt acggtttgtc atccaatagt
     4141 ggggaaacac tgaaacagct ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac
     4201 atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt taatggcaga taaagtgttg
     4261 ccagtggaaa tgccattcag tgccgtctat tacagcgacc gtgcggtaag tacgcgcatc
     4321 cgggctttta tcgatttcct tagcgagcat gtaaaaacag ctcccggagg agctgtcaga
     4381 gaggcttaat cccattcagg agccagacct tccgggctaa ccaggcggtc gttgcaatcc
     4441 agtgcggcga tcgctttttt atcttcggca tcaagctgta aattttgtgc cttaagatta
     4501 ctttccaggt ttttacgttt agtagaagaa ggaattactg agtaaccttc ccccatagcc
     4561 cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag ctgcgatacg agcaataacc
     4621 tcatctttca gggccttacc atacgccagc gtcatatagg aagtaatatg gatgccgtgc
     4681 tgtttagccc aggcaaccac tttacggttt tgcagataag gagagagttc aatctggtta
     4741 gtagcgatgt tttcagcacc aacagcagca atcgcttttt ccatcaacgg gatcgtgaag
     4801 ttggaaatac cgatctcacg cgtcagccct tgttttttgg cttccagcag cgcctgcata
     4861 aactcttcaa cagagacttc atcgtttggt gacggccagt ggattagcgt cagatcaaca
     4921 taatcggtac gcaatttttg caggctctct ttcagacttg ggatcaattt gtctttgctg
     4981 agattttcaa tccagatttt agtggtgatg tagagttcat gacgtggcac gccactttct
     5041 gcaatcgcct gacctactgc ggcttcgtta tcatagattt gtgcggtatc aattgcgcga
     5101 taaccaagtt caagcgccgt tatcacagat gaaataacaa cgtcgtcttt cagacggaaa
     5161 gtacctaaac caaatgcagg gatagccata atatgcctct tttaatgcta ttttcgttaa
     5221 ctgaggtgat tatgggcttc agactgttga ggaaaaagag cgtaaaatgc agaggatttt
     5281 tgcgattctg gcaataatag atatacagac gaaaaaaaac ctcgagcgtt aactcgaggc
     5341 ttcttaataa gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
     5401 gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAPHJE010000203         5402 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPHJE010000203 DAPHJE010000000
VERSION     DAPHJE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.205"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            198..1334
                     /locus_tag="RH222_004294"
     CDS             198..1334
                     /locus_tag="RH222_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3799213.1"
                     /translation="MPILELRFSNKSLHQLPHPLTSRQEYRDIHCQNLRALVYPNRIT
                     LAFRATINNQRIYETLGQFPQLCVEDARQHVMKLLADKNRLAVQSRRFTVEQAINDLW
                     LPDLQLYKKSPQSELSKLNLYVRPFWGKRQLRDITAGELEQYAAGLREKLRPSTVNRI
                     LAILSKICSLAVRAGWITHSPMTHVRYLKENNIRYRTLSATEIPRFIDAATALQTPAA
                     DSILLALYTGMRIGEVCTLKWEYWHPDMHQLILPDTKSGHPFTCPLAPPAIAVVECQQ
                     DLMLSKTYIFPQLTDNARPLAYPRATFARICRDANIDNLRMHDLRRTFATRVLQATGD
                     IAIASHLLNHHSLTATRRYAFHNAATSHAVVSQVIESYDTKMNL"
     gene            1477..1677
                     /locus_tag="RH222_004295"
     CDS             1477..1677
                     /locus_tag="RH222_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3799214.1"
                     /translation="MYLTIKQFAERFNLSASTVASDISRNPGKLPPFIRIGRAIRFSL
                     DDIVEWENAHRQNKLKGDINGY"
     gene            1667..2305
                     /locus_tag="RH222_004296"
     CDS             1667..2305
                     /locus_tag="RH222_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799215.1"
                     /translation="MVTKNTANNANNALNILPEAANTAVDNDEKYLSFALVLAITIMG
                     NLVKLIGTDGFVLYTYTLQDTATARAVFNELARRLKNFNRQEEVYTTDSLTFRMKYIY
                     GVTLFEHDSKSILNLFDKKGYPVLSESGEPGSLDDMYLDIQARLHGGYASKKFLHLHE
                     HCLLSAHVTPSVEKTQRGILIKAGRKLVSFIHVDDESHKTDIFKSVVNVIKS"
     gene            2352..4952
                     /locus_tag="RH222_004297"
     CDS             2352..4952
                     /locus_tag="RH222_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799216.1"
                     /translation="MSNNLQDILAAASGYQSVTSEPALNLKRPKTLDDYPVIPPASKK
                     VSVISSDLTLHIGFDTEYVFNPETRQNDILSYQSYVVLPDNTGVSNIIYPPDSQKKSR
                     LSFKDFLCQTITPLLETGVITKWPGIINIYAHFIRADITSFANFWSDYKILLKGIRGT
                     VSSFKNRYGIDFDEQQERRVKTEQIMFDKRTSPPRCSNVAFIDTLLITPGGMGLAECG
                     ELLGLPKLTIPAPYSITNMREYLLGDRAGFEAYALRDAEIAVRYALQVRNFCARELMI
                     DRVPATIGAMAVSRFTKTLKENNMSPEVCLGTHIKTRELWLTEKQAFRTIKNPASVPS
                     RELFETFPINCYHGGRNECFMMGVTPSDHWYDYDLAGAYTTGLLDILTPDYGNIRLSK
                     NPDDYCGHVMGFALVTFRFPESVPYPSLPVRTDQYGLFFPLSGESWATAPEIELALSL
                     GAEMTIHNGIIVPWICDTSPHNSESTSVFLPFVQQVRENRNRHIKGSLEEKFWKEIGN
                     SLYGKLAQGLRAKTAFDTARGLNRSLPPSSVTQPFFAAHVTGFIRAVVGELMNALPSD
                     SSVVSVTTDGFLTNCPLNKINMSGPLSSRFQSLCDIVDPGSSMLTCKHEVSQLIAMKT
                     RGQLTYRAIQGKPVVHARAGVKPPADIPRSDYNDYMVDLYLNRLPGQTLSRSTLISTR
                     EMWLSESDLVSREQDIRLNLEFDFKRQPVQPAMNEGHLLMSSRPWDNMEEALQQRSLF
                     DDWRQTHTLKTLADWDDWCDFLYCRTVFSDMKLKVGSKRSDDILVRLFLRALTQCQWG
                     LMLKDKKSYSCKEVAEWLTSEGYSVTVTDVKNAVRAKIPQMKFGSVTPRMKSLMDIIA
                     RKYPTFCLPA"
     gene            complement(5001..5327)
                     /locus_tag="RH222_004298"
     CDS             complement(5001..5327)
                     /locus_tag="RH222_004298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799217.1"
                     /translation="MYLHIRKYQLVNARWRDIIDLTDYEETIRQVVGTLFGNDFIEVS
                     VETNCFTLTVNSTSSKIPHGILVNMGKRLAANLQSITCHAMRIYHVNGHPDARQLFHC
                     FDADCL"
BASE COUNT         1459 a         1312 c         1146 g         1485 t
ORIGIN      
        1 tgataaattt atggagttag ccagaaggaa taacccatta aaaatgtaac gcatgctttt
       61 gaaaatattt ccagtaattg tacgcataac ttttcattgc attcattatt tttcacctga
      121 tgaactgtga aaactgagac tggcatacag taatctcgca ccgtattttt tccgtgttat
      181 tttacaggag aaatacgatg cctattctgg aactgcgttt cagcaacaaa tcacttcacc
      241 aacttcccca tcccctcacg agccgtcagg aataccgtga tattcactgc caaaacctgc
      301 gtgctctggt gtatcccaat cggatcacgc tggcctttcg tgccactata aacaatcagc
      361 gtatatatga aaccctgggt cagttccctc agctctgcgt tgaggatgcc cgtcagcatg
      421 tgatgaaact gcttgctgat aaaaatcgtc ttgcggttca gtcccgtcga ttcactgtgg
      481 aacaggccat caatgacctg tggctaccgg atttgcagtt gtataaaaag agtcctcagt
      541 cagagttgtc aaaactcaac ctctacgttc gcccgttctg gggcaagcgg caacttcgcg
      601 acatcacggc aggagaacta gaacaatatg ctgctggctt acgggaaaaa ttacgtccgt
      661 caacagtaaa ccggatcctc gcaatattgt ccaaaatatg ctctctggca gtacgtgctg
      721 gatggatcac gcattccccg atgacacatg tccgctatct gaaagaaaat aacattcgct
      781 atcgcacact gtcagcgaca gaaatcccgc gctttattga cgcggcaaca gcgctgcaaa
      841 caccggcagc tgattctata ttgcttgctc tttatacggg gatgcgtatt ggtgaggtat
      901 gtaccctgaa atgggagtac tggcacccgg atatgcatca actcatactg cctgatacaa
      961 agtccggaca ccctttcacc tgcccacttg cacctccagc cattgcagtt gtcgagtgcc
     1021 agcaagacct gatgctcagc aaaacttaca tttttccaca actaaccgac aatgcccgcc
     1081 ctctggcata tccgcgtgca acctttgccc gtatttgtcg ggatgccaac attgataacc
     1141 tgcggatgca tgacctgcgc cgtacctttg ccacccgggt actacaggcg accggggata
     1201 ttgcaatcgc ttcccatctg ctaaatcacc attcactcac ggcgacccgt cgctacgctt
     1261 ttcacaatgc agcgacatcg cacgcagtgg tttctcaggt catagaaagt tatgacacaa
     1321 aaatgaacct atgatctgtg attcgtgatt gatgcaacac tcgtttaagg acggtattgt
     1381 cattgcatgc ttctgcaggg ccctgcatta agtcatcaaa gaatgtggat tgatattaaa
     1441 tccgcgtggt ttcttcttct ctaaaaccgg aggtttatgt atttaaccat taagcagttt
     1501 gcagaaagat ttaatctttc cgcatccacg gttgcgtcag atatatcgcg caacccagga
     1561 aaactcccgc ccttcatcag aattgggcgg gctatacgat tctctcttga tgatattgtt
     1621 gagtgggaaa atgcacatcg tcaaaacaaa cttaaaggtg atataaatgg ttactaagaa
     1681 cacagcaaat aatgctaata atgcactgaa cattcttcct gaagcagcaa acacagctgt
     1741 agacaacgat gaaaaatatc tgtcctttgc attagttctc gctatcacga ttatgggcaa
     1801 cttagtaaag ctcatcggca cagatggctt cgtactttat acctacaccc tgcaggacac
     1861 ggctactgct cgtgctgttt ttaatgaatt agccaggcgt ctcaaaaatt tcaatcgtca
     1921 ggaggaggtc tacaccaccg attccctcac ctttcgaatg aaatacattt acggtgttac
     1981 cctgttcgaa catgatagta aatctatcct taacctcttt gataagaagg gttatccggt
     2041 attgagtgaa tcaggagaac ccggttctct tgatgatatg tatcttgaca ttcaagcccg
     2101 attacatggc ggctacgcct caaaaaagtt tcttcattta catgaacatt gtctgttatc
     2161 tgcacacgtt accccgtctg tggaaaagac gcaacgcggt atcctgatta aagcaggcag
     2221 gaaactcgtt tcctttatcc atgtagatga tgaatcacac aaaaccgata ttttcaaatc
     2281 tgtagtaaat gttattaaat cttgattctt tatgccggga cacagctccc ggcatttttt
     2341 cggagttaac tatgagcaat aatttgcagg atatcctcgc tgccgcctcg gggtatcagt
     2401 ccgtgacttc agagccagcg ctcaatctta aaagacctaa aacgcttgat gattatcctg
     2461 tgataccacc tgcatcaaaa aaagtatctg ttatttcaag tgacctgaca ctccatatcg
     2521 gttttgatac cgaatatgtt ttcaaccctg aaacacgaca gaatgatatt ctgtcatatc
     2581 agagttatgt cgtcttaccc gataacactg gtgtatcgaa cattatctat ccacctgact
     2641 cacagaaaaa atcccgactg tcttttaagg actttctctg tcaaacgatt acaccgcttc
     2701 ttgaaacggg tgtgattaca aaatggcccg gtattattaa tatatatgcg catttcattc
     2761 gtgcggatat cacctccttt gccaatttct ggtcagacta caaaattctt ctgaaaggaa
     2821 tacgcggtac tgtcagcagc ttcaaaaatc gttacggtat cgattttgat gaacagcagg
     2881 aacgtcgcgt aaagactgaa cagataatgt ttgacaaacg aacttcccct ccgcgttgca
     2941 gtaacgtagc tttcatcgat acactgctta tcactcccgg gggtatgggt ctggctgaat
     3001 gtggtgaatt actgggtctt cccaaactca ccattcctgc accttattcc ataaccaata
     3061 tgcgtgaata tcttctgggc gacagagcag ggtttgaagc ctatgctctg cgggatgccg
     3121 agattgcggt tcgttatgct ttacaggttc gcaatttttg cgcccgggag ctgatgattg
     3181 atcgtgtccc cgccaccatc ggggcgatgg cagtttcacg gttcaccaaa acacttaaag
     3241 aaaacaatat gtctcctgaa gtctgccttg ggacacacat taaaacccgt gaactctggc
     3301 tcacggagaa acaggcattc cggacgatta aaaaccccgc cagtgttcct tcaagagagt
     3361 tgtttgaaac gttcccgatc aactgttacc acggcggacg caatgagtgt tttatgatgg
     3421 gggttacacc gtcagatcac tggtatgact acgatctggc tggagcgtat accaccgggc
     3481 tattagatat cctcacaccc gattacggga acatccgcct cagtaaaaat cctgacgatt
     3541 actgtgggca tgtaatggga tttgcgcttg tcacatttcg gttcccggaa tcagttccct
     3601 atcccagcct gccggttcgg accgatcaat atgggttatt ctttccatta agtggcgaat
     3661 catgggcaac ggcacctgaa atcgaacttg ctctgtcgct cggggcagag atgacgatcc
     3721 ataatgggat catcgtcccc tggatctgtg acacctcccc acataacagc gaatcaacat
     3781 cggtgtttct tccctttgtt caacaggtcc gtgagaaccg taatcgccat attaaaggtt
     3841 ctctggagga gaaattctgg aaagaaattg gcaactcgct ttatggcaaa cttgctcagg
     3901 gccttcgcgc caaaaccgcc tttgatactg cccgtggatt aaacagatcc ctcccaccat
     3961 catcggttac tcagccattc tttgctgcgc atgtgacggg atttatccgc gccgttgtcg
     4021 gggaactcat gaatgctttg ccttctgatt catcggttgt cagcgtcaca accgatggct
     4081 ttttgacaaa ttgcccactt aacaagataa acatgagcgg tccactgtct tcccgcttcc
     4141 agtcgctgtg cgatattgtt gatccgggtt catcaatgtt gacatgtaag catgaggtca
     4201 gccagcttat cgccatgaaa acccgggggc agttaaccta tagagctatt cagggaaaac
     4261 ctgttgttca tgccagggct ggcgtcaaac ctcctgccga tattccacgc agtgactata
     4321 acgactatat ggtagatctt taccttaatc gtttacccgg tcagacgtta tcccgaagta
     4381 cgttgatatc aacgcgtgag atgtggctgt cggaaagcga tctggtatcc cgggaacagg
     4441 atatccgtct taatctggaa ttcgatttca aacgccagcc cgtacagccc gccatgaatg
     4501 aagggcattt actgatgtct tcccgcccat gggataatat ggaagaggct ctgcagcaac
     4561 gctctctttt cgacgactgg cggcaaacgc atacactgaa gaccctggct gactgggatg
     4621 actggtgtga ttttctctat tgccggacgg tgttttctga tatgaaacta aaagtcggaa
     4681 gcaaacgtag tgatgatatt ttagtcagac tatttctccg ggcgctgact cagtgccaat
     4741 gggggctgat gctgaaagat aaaaaaagtt attcctgtaa agaggttgct gaatggctga
     4801 cgtcagaagg ctattcagta acggtgactg atgtaaaaaa tgcagtaaga gcaaaaatac
     4861 cccaaatgaa atttggctca gtaacaccgc gaatgaagtc actgatggat atcatagccc
     4921 gtaaatatcc gacattctgt cttccagcct gataaccgtt aaatctcccc caactccttt
     4981 aacagaagtt gggggcttaa tcacaggcaa tcggcatcaa aacagtgaaa taactgccgc
     5041 gcgtcaggat ggccatttac atgatagatc cgcatggcat gacaggtgat actctggaga
     5101 ttagccgcca gccttttccc catattcacc agtatgccgt gtggtatttt actgcttgta
     5161 ctgtttactg tcagagtaaa acaatttgtt tcaacgctga cctcaataaa atcatttcca
     5221 aataatgtac cgacaacctg cctgattgtt tcttcataat ctgtcagatc aatgatatca
     5281 cgccatctcg catttacgag ttgatacttt ctgatatgta aatacataga accaccttta
     5341 tgttatttca actaatatga tgttctccga tattatgaaa atattaatca tcattgtaat
     5401 tg
//
LOCUS       DAPHJE010000204         5358 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPHJE010000204 DAPHJE010000000
VERSION     DAPHJE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.206"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..853
                     /locus_tag="RH222_004299"
     CDS             155..853
                     /locus_tag="RH222_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3799218.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(898..1797)
                     /gene="eamA"
                     /locus_tag="RH222_004300"
     CDS             complement(898..1797)
                     /gene="eamA"
                     /locus_tag="RH222_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV3799219.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1992..3179
                     /gene="ydeE"
                     /locus_tag="RH222_004301"
     CDS             1992..3179
                     /gene="ydeE"
                     /locus_tag="RH222_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV3799220.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3306..3401
                     /gene="mgtS"
                     /locus_tag="RH222_004302"
     CDS             3306..3401
                     /gene="mgtS"
                     /locus_tag="RH222_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV3799221.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3620..4510)
                     /gene="dgcZ"
                     /locus_tag="RH222_004303"
     CDS             complement(3620..4510)
                     /gene="dgcZ"
                     /locus_tag="RH222_004303"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV3799222.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLKGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4765..5157)
                     /gene="ydeI"
                     /locus_tag="RH222_004304"
     CDS             complement(4765..5157)
                     /gene="ydeI"
                     /locus_tag="RH222_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV3799223.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1490 a         1271 c         1122 g         1475 t
ORIGIN      
        1 atatcccgca gtagctatac gtcctggtac attgatcaca gtaatacagt gataaagttg
       61 ttcaacatca gggtgattat taaacgactc ggccatacga taaaccaatc aggactcaac
      121 tccttgtttt caatggctct taaaaggtgc aaaagtgatt tttcaaaaga ttgcccgctt
      181 acttaaatcc gaaatcaatg gcaattcatg gcatgttggt gatttgttgc cgtcagaagc
      241 ggaactcgct gttcgttata atgtttcgcg taataccctg cgtaaggcat tatccttgct
      301 ggaaggcgaa ggaattattc acagaaagca tggttcagga acatacattc aaaaaaagaa
      361 ttttgttgca cacattgatc acatgaacag tttcagtgaa attgcgcata aaagcggcaa
      421 agaggcaggg agtcagatta tgaaatttga agtgcaggat gcttcaccca ctattgcgac
      481 tgagctgaat ttagtgactg gtgagcaggt ttattacata aagagactgc gattcattga
      541 ggataatgca gcacaattag aagaaacgtg gatgtcagta gcacgttttc ctgatttaac
      601 cgtgtcacat atgcaaaaat ccaaattttc atatattgag aatgaatgcg ggatcaaaat
      661 cattggcacc tttgaaactt tctcccctac ttttcctacc ccagaaatcg ccagtatttt
      721 acggatcagc ccacgggatc ccatacttaa aattcagacc caggctgtgg acagtaactc
      781 tattccgctg gattattcgt tactttacag caatattttc gagttccagg taaagtactt
      841 tttcccgcga taaaagcaga cgcacagagt gctgacatca cattctgtgc gtatggttta
      901 gcctctaacc tttaccgctt tacgccaccg caagccaaat acattgatat acagcccggt
      961 cataatgagc accgcaccta aaaattgcag acccgttaag cgttcatcca acaatagtgc
     1021 cgcacttgcc agtccgacta cgggcaccag taacgataac ggtgcaaccc gccaggtttc
     1081 atagcgtccc agtaacgtcc cccagatccc ataaccaaca attgtcgcca caaacgccag
     1141 atacatcagc gacaagatgg tggtcatatc aatagtaacc agactgtgaa tcatggatgc
     1201 ggaaccatcg agaatcagcg aggcaacaaa gaagggaatg attgggatta aagcgctcca
     1261 gattaccagc gacatcaccg ccggacgcgt tgagtgcgac atgatctttt tattgaagat
     1321 gttgccacac gcccaactaa atgctgcagc cagggtcaac ataaagccga gcatcgccac
     1381 atgctgaccg ttcagactat cttcgattaa caccagtacg ccaaaaatcg ctaaggcgat
     1441 ccccgccaat tgtttgccat gcaatcgctc cccgaaagta aacgcgccaa gcacgatagt
     1501 aaaaaacgcc tgtgcctgta acaccagcga agccagtcca gcaggcatac cgaagttaat
     1561 ggcacaaaaa agaaaagcaa actgcgcaaa actgatggtt aatccatacc ccagcagcaa
     1621 attcagtggt actttcggtc gtgcgacaaa aaagatagcc ggaaaagcga ccagcataaa
     1681 gcgcaaaccg gccagcatca gcggtggcat gttatgaagc cccactttga tgaccacaaa
     1741 atttagcccc catacgacca ctaccaatag cgccaacacc ccatcttttc gcgacattct
     1801 accgcctctg aatttcatct tttgtaagca atcaacttag ctgaatttac ttttctttaa
     1861 cagttgattc gttagtcgcc ggttacgacg gcattaatgc gcaaataagt cgctatactt
     1921 cggatttttg ccatgctatt tctttacatc tctaaaacaa aacataacga aacgcactgc
     1981 cggacagaca aatgaactta tccctacgac gctctaccag cgcccttctt gcctcgtcgt
     2041 tgttattaac catcggacgc ggcgctacgc tgccatttat gaccatttac ttgagtcgcc
     2101 agtacagcct gagtgtcgat ctaatcggtt atgcgatgac aattgcgctc actattggcg
     2161 tcgtttttag cctcggtttt ggtatcctgg cggataagtt cgacaagaaa cgctatatgt
     2221 tactggcaat taccgccttc gccagcggtt ttattgccat tcctttagtg aataacgtaa
     2281 agctggtggt gctctttttt gccctcatta actgcgccta ttctgttttt gctaccgtgc
     2341 tgaaagcctg gtttgccgac aatctttcgt ccaccagcaa aacgaaaatc ttctcaatca
     2401 actacaccat gctaaacatt ggctggacca tcggtccgcc gctcggcacg ctgttggtga
     2461 tgcagagcat caatctgccc ttctggctgg cagctatctg ttccgcgttt cccatgcttt
     2521 tcattcaaat ttgggtaaag cgcagcgaga aaatcatcgc cacggaaaca ggcagtgtct
     2581 ggtcgccgaa agttttatta caagataaag cactgttgtg gtttacctgc tctggttttc
     2641 tggcttcttt tgtaagcggc gcatttgctt catgcatttc acaatatgtg atggtgattg
     2701 ctgatgggga ttttgccgaa aaggtggtcg cggttgttct tccggtgaat gctgccatgg
     2761 tggttacgtt gcaatattcc gtgggccgcc gacttaaccc ggctaacatc cgcgcactga
     2821 tgacagcagg caccctatgt ttcgtcatcg gtctggtcgg ttttattttt tccggcaaca
     2881 gcctgctatt gtggggtatg tcagctgcgg tatttactgt cggtgaaatc atttatgcgc
     2941 cgggcgagta tatgttgatt gaccatattg cgccgccagg aatgaaagcc agctattttt
     3001 ccgcccagtc tttaggctgg cttggtgccg cgattaaccc attagtgagt ggcgtagtgc
     3061 taaccagcct gccgccttcc tcgctgtttg tcatcttagc gttggtgatc attgctgcgt
     3121 gggtactgat gttaaaaggg attcgtgcaa gaccgtgggg gcagcccgcg ctttgttgat
     3181 ttaagtcgaa cacaataaaa atttaattca gccttcgttt aggttacctc tgctaatatc
     3241 tttctcattg agatgaaaat taaggtaagc gaggaaacac accacaccat aaacggaggc
     3301 aaataatgct gggtaatatg aatgttttta tggccgtact gggaataatt ttattttctg
     3361 gttttctggc cgcgtatttc agccacaaat gggatgacta atgaacggag ataatccctc
     3421 acctaaccgg ccccttgtta cagttgtgta caaggggcct gatttttatg acggcgaaaa
     3481 aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata gatttgtgtt ttgcttttac
     3541 gctaacaggc attttcctgc actgataacg gatcgttgac acagtagcat cagttttctc
     3601 aatgaatgtt aaacggagct taaactcggt taatcacatt ttgttcatca ataaacatgc
     3661 agcgatttct tccggtttgc ttaccctcat acattgcccg gtctgctctt ccaatgacca
     3721 catccagagg ctcttcagga aatgcgcgac tgacacctgc tgtcacggta atgttgatat
     3781 gcccttcaga atgcgtgatg gcatggttat cgactaactg gcaaattctg acacctgcac
     3841 gacatgcttc ttcatcatta gccgctttga caataatgat aaattcttcg cccccgtagc
     3901 gataaaccgt ttcgtaatca cgcgtccaac tggctaagta agttgccagg gtgcgtaata
     3961 ctacatcgcc gattaaatgc ccgtaggtat cattaaccaa tttaaatcgg tcaatatcca
     4021 acaacattaa ataaagattc agaggctcag tgttgcgtaa ctgatgatca aaggattcat
     4081 caagaacccg acgccccggc aatcccttca aaacatccat attgctacgg atcgtcagca
     4141 aataaatttt gtaatcggtt aatgccgcag taaaagaaag caacccctcc tgaaaggcgt
     4201 cgaaatgcgc gtcctgccag tgattttcaa caatagccag cattaattcc cgaccacagt
     4261 tatgcatatg ttgatgggca gaatccatta gccgaacgta aggtaattca tcgttatcga
     4321 gtggccccag atgatcaatc caccgaccaa actggcacag tccataagaa tggttatccg
     4381 ttatttctgg cttactggca tctctcgcga ccacgctgtg aaacatactc accagccact
     4441 ggtagtgggc atcgatagcc ttattgagat ttaacaagat ggcatcaatt tccgttgtct
     4501 tcttgatcat tgccactcct ttttcacagt tccttgtgcg cgctattcta acgagagaaa
     4561 agcaaaatta cgtcaatatt ttcatagaaa tccgaagtta tgagtcatct ctgagataac
     4621 attgtgattt aaaacaaaat cagcggataa aaaagtgttt aattctgtaa atcacctctg
     4681 cattatcgta aataaaagga tgacaaatag cataacccaa taccctaatg gcccagtagt
     4741 tcaggccatc aggctaattt atttttattt ctgcaaatga gtgacccgaa cgacggccgg
     4801 cgcgcttttc ttatccagac tgccgctaat gttgatcatc tggtccggct gaacttctcg
     4861 tccatcaaag acggcagcag gaataacgac attaatttca ccgctcttat cgcgaaaaac
     4921 gtaacggtcc tctcctttgt gagaaatcaa attaccgcgt agtgaaaccg aagcgccatc
     4981 gtgcatgttt tttgcgaaat caacggtcat tttttttgca tcatcggttc cgcgatagcc
     5041 atcttctatt gcatgaggcg gcggtggcgc tgcatcctgt tttaaaccgc cctggtcatc
     5101 tgccaacgca taaggcatga caagaaaact tgctaataca atggcctgaa atttcatact
     5161 aactccttaa ttgcgtttgg tttgacttat taagtctggt tgctattttt ataattgcca
     5221 aataagaata ctgccaattg ttataaggca tttaaaatca gcccactagc tgtcaaatat
     5281 tcagagaatt taactcacta aagttaagaa gattgaagag tcttaaacat attttcagaa
     5341 taatcggatt tatatgtt
//
LOCUS       DAPHJE010000205         5131 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPHJE010000205 DAPHJE010000000
VERSION     DAPHJE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.207"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            54..1310
                     /locus_tag="RH222_004305"
     CDS             54..1310
                     /locus_tag="RH222_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3799224.1"
                     /translation="MSYCREHGGFSLSTLRLFIEKEDIDNATFYSVLFGLKILCAETF
                     PGFNLEDYDDLEFVPRPYLEHWGVYQEIDNILDPLEKNMICNGLFEMASLLRDGKSFS
                     HSEVRNAAILGLAYVTGARPVQLARLAVKDVRIDTRNQESGLIRYSVFLPYAKQRRVT
                     TERLFLAIPPEIGELIMNYTVRYKKNPEDKLFDFSVSAPHYVSQAINQAILNFCPPEY
                     QAAVACGEAALPTITPTDLRHNVGHSLAMQGASAEEIAHVLGHTSLAVAKYYIMATPA
                     LALIRAKALGSNPVWQNMVAMMLTGELVDSAHWKGHPVVGLVGDELHDKIGGCSRNSS
                     TCPFSEVRSCYGCLYYRPFTDGEHQALLECVKKEVDELIAISDGVGNSRNPLILIHET
                     TQFEIESVIARCRFHQEQVKSNEKSL"
     gene            1294..3660
                     /locus_tag="RH222_004306"
     CDS             1294..3660
                     /locus_tag="RH222_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799225.1"
                     /translation="MKNHFEHFQAFIEEQKKWFEQHLSADFAQTWNDHIWVSGNQGSG
                     WLRGNGRNTLRFDEMNRFKGIVEHLPIDKEYSQFMKAMLVVVYRQGNRSMSPAVAKAT
                     LMILKRWYHAMLYETGQTHPSYLSTVVIQRAMDLLSSVSSPSDPNTANYKGRCVSLQK
                     LVNHFTFTLVKLQYNSDVKYTNKTNLTRKARETMALKQQAKLSDTTTDGEDALITIRG
                     FLNIVALIQRVESDAEKIALNCLLLLVITGFRSIEAFNLRQDALFKRQIDDPAICKRF
                     QDKGLPDYFLGIRYVGVKGAGERTHWVEPLAVPLVESIFSTVKMLTAPIRSHLTYLRA
                     KSFADYLPQAISNMPGERVELDDVVKYITQTTSSFRGRAGQRDKTSKALSKRGVLPVQ
                     EIPGPKNSKSIYYSKTALNHYIKTEFGLANANEPCTHAWMENGKRYQVNYEDLLFLHE
                     KGSLALKRTLALLATPIPFTNTLMNKFLGNVEPDGSVFSKYQLLEEDGTSTRMRTHIP
                     RHNINTFLAIADISDHLQAMLMGRVDITQNQHYQHLALAERRKAASLVPLQPISTALT
                     VSPSSSSIATPLDIVKRTGQLAVTEHMTLDNTIKANLHTFDDRDDVARFVEASFANGL
                     FEDVAAAFEEIRDAEGPEQASDMVARHAVLYPLKFGSCMREVNLWGCPYRLKCQSAAF
                     CEHFTLTGRIDELPNLIAKKQALQQAHSKLTQLTQHQPDYQTRLADIERRLQQLEAIQ
                     AQWHRRAKTQQLVAAESVLSGGVITEGKVRTLAQLFALEYQQLMQEND"
     gene            3660..4094
                     /locus_tag="RH222_004307"
     CDS             3660..4094
                     /locus_tag="RH222_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799226.1"
                     /translation="MRGKELNTLIEHELQLMLVEGFDKSPISAKALHIRLKAKGIVNG
                     GLSTLSSLERKRLIAAYVDQQLGPLNLRPKEKQQYVNRKTRQALLARNQQLQAEVSEL
                     RDQLAQNTLSLIEIVKAVKINSVIPVESLLANHMIRELHKSS"
     gene            complement(4299..4631)
                     /locus_tag="RH222_004308"
     CDS             complement(4299..4631)
                     /locus_tag="RH222_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3799227.1"
                     /translation="MSEKNPIPERLKEARSRAGLSQRSLGLLVGFDPASASSRMNHYE
                     KGRHVPDIDTLRRMAAELNVPLNYFFCDDQTTAELALLISRMTEEERSNLIEALKRHP
                     GEKHADKK"
BASE COUNT         1444 a         1158 c         1163 g         1366 t
ORIGIN      
        1 cgacgagatt tccaaccaaa cttccagcgt tgcaccttga ttggaggaat atcatttcat
       61 attgtcgaga acatggtggg ttttcgctta gtacgctccg attatttatc gaaaaagagg
      121 atattgataa tgctactttt tattccgtgt tattcggatt aaaaatactt tgtgctgaaa
      181 cattcccagg tttcaatctt gaagattacg atgatcttga gttcgtccct cgaccgtatc
      241 tcgaacattg gggggtttac caagaaatcg ataatattct tgaccctctt gaaaaaaaca
      301 tgatttgcaa tggactgttt gaaatggctt cgttgttacg cgatggcaaa agtttcagtc
      361 acagcgaagt gcgcaatgca gctatccttg ggttggctta tgtaacgggt gcacgcccag
      421 tacaactcgc taggctggcg gtcaaagatg ttcgtatcga tacacgaaat caggaatctg
      481 gtctgattcg atacagtgtt tttttaccat atgctaaaca aagacgagtg acgacagagc
      541 ggttatttct ggcgatcccc ccagaaattg gggagttaat tatgaactat accgtgagat
      601 ataagaaaaa ccccgaagat aaactattcg atttcagcgt ttcagctccg cattatgtgt
      661 ctcaggcaat taatcaggca atcctaaatt tctgtcctcc tgaatatcaa gccgccgtag
      721 catgtggcga agctgcattg cccacaatta cacccactga tttacgccac aatgttgggc
      781 actctttggc gatgcaaggt gctagcgctg aagagatagc acatgtactg gggcacacct
      841 ctcttgccgt agctaaatac tacattatgg caactcccgc gttggccttg atccgtgcta
      901 aagcgcttgg gtcgaatccc gtttggcaaa atatggtggc tatgatgctc actggggaac
      961 tggtcgactc agcacattgg aaaggccatc cagtggtggg actcgttggt gacgagttgc
     1021 atgataaaat tggtggttgc tccaggaaca gtagtacatg ccctttcagc gaagtgcgtt
     1081 cttgttacgg ctgtctgtat tatcgccctt ttacggatgg tgaacatcag gctctacttg
     1141 aatgcgtcaa aaaagaagtc gatgaactaa tcgctatttc agacggtgta ggtaattccc
     1201 gaaatccgct cattttaatc catgaaacca cacagtttga aattgagtct gttatcgccc
     1261 gctgccgttt tcatcaggaa caggtgaagt ccaatgaaaa atcactttga gcattttcag
     1321 gcttttattg aagagcaaaa aaaatggttc gaacagcacc tgtctgctga ttttgcccaa
     1381 acatggaatg atcatatctg ggtaagtggt aaccagggaa gtggatggtt gcgagggaac
     1441 ggcagaaata cattacgttt tgatgagatg aacagattca aaggtattgt agaacatctg
     1501 cccatcgaca aagaatatag tcagttcatg aaagctatgc tggtagtggt ttaccgacag
     1561 ggaaatagga gtatgtcgcc agcggtggca aaggcaactt tgatgattct gaaacgatgg
     1621 taccatgcga tgctttatga gacaggacag acccatccat cttacctgag cacagttgtc
     1681 attcaacgag cgatggatct tctcagtagc gtttcatctc ctagtgaccc caatacagct
     1741 aactataaag ggcggtgcgt tagccttcaa aaattggtca atcacttcac atttacgttg
     1801 gttaaactgc aatacaactc tgatgtgaag tataccaaca aaaccaactt gacccgcaaa
     1861 gcccgggaaa ccatggcgct gaaacagcag gccaagctgt ctgataccac taccgatggt
     1921 gaagatgcgt tgattaccat tcgcggattt ctgaacatag ttgcattaat ccagcgggtg
     1981 gaaagtgatg ctgagaaaat cgcgttaaat tgcctgttgt tattggttat taccggcttt
     2041 cgctccattg aagcgtttaa tctcagacag gatgccctgt ttaaacgcca gattgatgat
     2101 ccggcaatct gcaaacgatt tcaggacaag ggattacccg attattttct tggtatccgt
     2161 tatgttggtg tcaaaggtgc tggggagcga acgcattggg tagaaccgct ggcagtaccg
     2221 ctggtcgaga gtatattcag taccgttaaa atgttgaccg caccaatacg cagccatctt
     2281 acctatctac gggcaaaatc ttttgctgat tacctgccac aggccatcag caacatgccc
     2341 ggtgaacgag tcgaactgga cgatgtggtg aagtacatca cccaaactac ctcaagtttt
     2401 cggggccggg caggacaacg ggacaaaaca tccaaagccc ttagcaaaag aggtgtgctg
     2461 cccgttcaag aaataccagg cccgaaaaac agcaaatcta tctattattc caagactgct
     2521 ctcaatcatt acattaagac cgaatttggc ctggcgaatg ccaacgaacc ctgtacacac
     2581 gcctggatgg aaaatggcaa acgctaccaa gttaactacg aggatctgct atttctacac
     2641 gagaagggtt cgctggcatt aaagcgcaca ctggctttgc tggctacacc gatacccttc
     2701 actaacacac ttatgaataa attcttaggc aacgttgagc ctgatggatc agtattcagc
     2761 aaatatcaat tactggagga ggatggcacc tcgacgcgga tgcgtaccca cattccccgg
     2821 cacaacatca atacctttct tgctattgcg gatatctctg accacttaca ggcaatgttg
     2881 atgggtcgag tggacatcac gcagaatcag cattatcaac atctggcgct tgcagaacgg
     2941 cgaaaagccg cctcgttggt gcccttgcag ccgatttcga cagcactaac tgtctcgccc
     3001 tcttcgtcct ccattgctac gccactggat atcgtcaaac ggaccggcca actggcggtc
     3061 acggaacaca tgactttgga caataccatc aaagccaacc tgcatacttt tgacgatcgg
     3121 gatgatgtag ccaggtttgt cgaggcgtca tttgctaacg gcctctttga agatgttgct
     3181 gccgcctttg aagaaatccg cgacgcagaa ggaccagagc aagcctctga catggtggca
     3241 agacatgccg tgctgtatcc gctgaaattt ggctcctgta tgcgggaagt taacctctgg
     3301 ggatgtcctt atcgactgaa atgccagagt gctgcctttt gtgaacattt cacgctgaca
     3361 ggccggatag atgagctacc aaatctgatt gccaaaaaac aggctttaca gcaagcacat
     3421 tcgaagttga cccaacttac tcaacatcag cctgattacc agaccagact ggccgacatc
     3481 gaacgacggt tacagcaact cgaggcaata caagcacaat ggcatcgtcg tgccaagact
     3541 caacaactgg ttgctgctga gagtgtgttg tctggtggag taatcaccga gggcaaagtc
     3601 cgtacactgg cccagctttt tgcacttgaa taccaacaat tgatgcagga gaatgattga
     3661 tgcgcggcaa ggaactgaac acactgattg agcacgaatt acaactgatg ctggttgaag
     3721 gctttgacaa gtcccctatt tccgccaagg ccctgcatat taggctcaag gcaaaaggta
     3781 tcgttaacgg tggtttaagt acgctgagca gcctagagcg taaacgccta attgctgcct
     3841 atgttgacca gcaacttggc ccattgaatc tacgcccaaa agaaaaacag caatacgtca
     3901 accgcaaaac ccgtcaggct ttgctggcca gaaaccagca gttgcaggct gaagtaagtg
     3961 aattgcgaga ccaattagcg caaaataccc tttcgttgat agaaattgtc aaagcggtaa
     4021 aaatcaattc ggttataccg gtggaaagcc tgttggccaa ccatatgatt cgggaactac
     4081 ataagtcgtc ataattctga catggaagat ttagccgaca gcttcccggt tgtcggcaga
     4141 atgctggttg tattgataaa catatattta tggctccctc ttttgagttt gttatctgag
     4201 agggcgtctc ccactgtaat gtcctgcttg gttcattgca tcagtgtaat gctacacttt
     4261 cgttaccttc aaaaagttca gtcgctttat atgtaatatc attttttgtc agcatgcttt
     4321 tcgcctggat gccttttgag tgcttcgata agattacttc gctcttcctc tgtcatccgg
     4381 gatatgagta atgcaagttc agctgtggtc tgatcatcac aaaaaaagta gttcaacggc
     4441 acgtttaact ctgccgccat ccgtcgaagc gtatcaatat cggggacatg gcgccctttc
     4501 tcataatgat tcatacgact gctagccgaa gcgggatcaa acccaacaag gaggcccaat
     4561 gagcgctgtg ataaacccgc cctgcttctc gcttccttaa gtctttctgg tatcggattt
     4621 ttttcagaca ctgattcatc ttcattggcc tcgaatgctt agattgtcta agtatctgcc
     4681 attgttgtat acttagcaat cctaagttaa ctgccttgtt ttagtcatat gaatagtcga
     4741 gtatccaacg ctgagtcgta catattttcc gcaataatct atattgctaa agataacttc
     4801 accagtaacg atgtggctca aattcttatc gagcgatttt cacttccgaa aagttatttt
     4861 aaggcaaaag cttttgctta taaccaaatt cagtgtttag ttagaaaagg attattgagt
     4921 aaagtcagaa aatcaggcgc gtatcaatat gtatactcag tcacatctga attcaatgtc
     4981 gtaaagaaaa gcgtagagtt aattgaggca cccaaaaaaa attccagtca acttgtttta
     5041 agtgctacca gtcatgaata cagaaatgta aatattcaaa taagaactct tattgaaaaa
     5101 tacagcagcg agttggaaaa agtatcagga g
//
LOCUS       DAPHJE010000206         5127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPHJE010000206 DAPHJE010000000
VERSION     DAPHJE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.208"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(14..1165)
                     /locus_tag="RH222_004309"
     CDS             complement(14..1165)
                     /locus_tag="RH222_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HDV3799228.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIPNILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(1236..1508)
                     /locus_tag="RH222_004310"
                     /pseudo
     CDS             complement(1236..1508)
                     /locus_tag="RH222_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1683..1805)
                     /locus_tag="RH222_004311"
     CDS             complement(1683..1805)
                     /locus_tag="RH222_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799229.1"
                     /translation="MMIILTGSIFTAVEENLCAAASGFSDATAHGLTATSALTT"
     gene            2095..3528
                     /locus_tag="RH222_004312"
     CDS             2095..3528
                     /locus_tag="RH222_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HDV3799230.1"
                     /translation="MIKITSIAVTLGLLSSLGAQAYAAGLVVNDNDLRNDLAWLSVRG
                     VIHLSLSTWPLSQEEIARALKKAKPSYSSEQVVLARINQRLSALKADFRVTGYTSTDQ
                     PGTPQGFGQTQPADNSLGLAFNNSGEWWDVHLQGNVEGGERISNGSRFNANGAYGAVK
                     FWNQWLSFGQVPQWWGPGYEGSLIRGDAMRPMTGFLMQRAEQAAPETWWLRWVGPWQY
                     QISASQMNQYTAVPHAKIIGGRFTFTPFQSLELGASRIMQWGGEGRPQSFSSFWDGFT
                     GHDNTGTDNEPGNQLAGFDFKFKLEPTLGWPVSFYGQMIGEDESGYLPSANMYLGGVE
                     GHHSWGKDAVNWYLEAHDTRTNMSRTNYSYTHHIYKDGYYQQGYPLGDAMGGDGQLVA
                     GKVELITEDNQRWSTRLVYAKVNPENQSINKAFPHADTLKGVQLGWSGDVYQSVRLNT
                     SLWYTNANNSDSDDVGASAGIEIPFSL"
     gene            3674..4807
                     /locus_tag="RH222_004313"
     CDS             3674..4807
                     /locus_tag="RH222_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV3799231.1"
                     /translation="MKKKIVRFSALALAIGFLSGCTVIPGQGLNSLRKNVVELPDSDY
                     DLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQDIASRLTTYIESPQ
                     VDVSVAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINNAGGLAPDADWRNVVLTHN
                     GKDTKVSLYALMQKGDLTQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TVAEALGNAEGMSQAFSDATGIFVIRQLKDDKQGKIANIYQLNAQDASAMVLGTEFQL
                     QPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETGKFIRTW"
     gene            4813..>5127
                     /locus_tag="RH222_004314"
     CDS             4813..>5127
                     /locus_tag="RH222_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152837.1"
                     /note="Wzb shows phosphatase activity towards the
                     autophosphorylated Wzc protein, which induces colanic acid
                     biosynthesis; catalyzes the phosphorylation of UDP-glucose
                     dehydrogenase, an enzyme involved in colanic acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HDV3799232.1"
                     /translation="MFNSILVVCTGNICRSPLGERYLRAALPEKKIDSAGTGALVGYQ
                     ADPTAIKIALEHDISLEGHIAKQFTSTMSRHYDLILVMEKYHIEKIGRIAPEARGKTM
                     LFG"
BASE COUNT         1290 a         1165 c         1277 g         1395 t
ORIGIN      
        1 cgctgttgta gattcaatct gtcaatgcaa cacccctttc aattatctct ttcggtgttt
       61 tgaacttcag tgtctttctc ggtctgttgt ttagctgagc agcaaccaga tctagttcat
      121 gttgagtata ttgggcaaga catgtctttt taggaaagta ctgccgaatt agcccatttg
      181 tgttctcatt tgttccccgc tgccaaggac tctgaggatc gcagaagtaa actttaacgc
      241 cggtgctgac agtaaattct agatgtctgg ccagttccat tcctctgtcc catgtcagtg
      301 attttctgag ttctgacggt aaactcagga atttgtcggt aagagcctga tttactgaga
      361 cagaatcttt gcccctgagt ctaaggatga tcgtataacg tgattttcgg tctacaagtg
      421 tggctatatg agagtttttt gtacctgaga ctaaatcgcc ctcccaatgc cccagagagc
      481 gtctgttatc gatatttcgg gaacgttcgt gaattggtgt tccgttcact atgttaatcg
      541 tacctctttc gcctttgcgg gtatgacgcc tgccatggcg aaggctatgc gaccgtcgca
      601 gatgctgtat attcaggtgg tgtagcgctt cacggctacg aaagtacagc gttttataaa
      661 ttgtctcagg tgatattcgc agcgtttttt gacgtggttt tgttcgcctt aaccatcctg
      721 atatttgctc tggagaccat ttcatctcca gcttttccag aacaagcttt cgcaatggta
      781 aattttgatc cagtaagcac ggttttggcc ttttcgccat tctgttggct cggttattag
      841 catcaacagc tttgtaatag cgtctgcccc gattacgctg aacttcacgt gagatcgtcg
      901 aaggactgcg attcagcgca gtagctatcg cacgaatgct cattttggct gacaaaccag
      961 ctcgtatctc ctcgcgctca gacagtgtca ggtgagctac agcccgctta cgctcatggg
     1021 gttttatgcc gccagtatcc cttaacatag tgaagatcgt tccgggtttt gaacccagga
     1081 tattcggtat ttcactgaag cctgttccgt tcttccatag ttcaaaaaca gaggcttttt
     1141 cctctgctgt aaatgttcgt ctcattcaaa aaacctccgc aaccccatgt tttcacataa
     1201 ctgttgcgtt gaccaattga atctacagca gcctgcttta gtatttcttt ttccatctca
     1261 aggcacttta tctgcgcttt aagctgctga atttcgcgtt gttcagggct aatagcatta
     1321 ccagcgggct caataccctg aagttcctgc ttatacaacc gtatccattt acgcaaatgg
     1381 tcagggttga gttcaagtgc cagcgcgcct tctctgacat cacgctggta tttaaccacc
     1441 acctgctcga aagcttcaag cttgaactcc gcggaaaagg tacgtttagt ccgacgagtt
     1501 ttgatcatgc atcacctcat tttcactgtt ttaacattaa caggatttcg aggtgtcctg
     1561 aattatcgat ccactacagt aacaacactc ccaattgtga ccgaaatccc gtaaacttag
     1621 cgcagccaaa aataatgacc tgaccaatta ttcatccaca ggtcgataaa aaattaagtc
     1681 gttcaggtag tcagtgcgct ggtagctgtt aagccatggg cggtagcgtc gctgaagccg
     1741 cttgctgctg cacagaggtt ctcttcaacg gctgtaaata tcgacccggt caggatgatc
     1801 atcatcaata gatatctatc aattttctat ggatttcctc agaagttgtt caggagcggc
     1861 tgctgatatc gcctggacat taactttcat gtctcattga tggcctgtag ggtaaattcg
     1921 tcgacattgt gagcttagtg gatgttataa ggctaacaag cagcttaggg taaatgtact
     1981 tgcctcgccg gtgttgcaca gcgaagtcag ctggtgccgc gagcgctctc tatcttggta
     2041 ttcccctcat tctcattgag atacgacagc agggctgaaa atggatcttg tacaatgata
     2101 aaaattacga gcattgccgt gacgttgggt ttgctttcct cactgggagc ccaggcttac
     2161 gcggccgggt tagtggtaaa tgacaacgac ttgcgtaacg acctggcctg gctttccgtt
     2221 cgtggggtca tccatctgag cctgtcgacg tggccgctga gccaggaaga gatcgcccgg
     2281 gcgctgaaaa aggccaaacc ttcctattct tctgagcaag tggtgctggc tcgtatcaac
     2341 cagcgactgt ctgctttaaa agccgatttc cgggtcaccg gctacacctc aaccgaccag
     2401 ccgggcactc cgcaggggtt tggccagacg cagccagcag ataactcgtt aggcctggcg
     2461 tttaacaaca gcggcgaatg gtgggatgtc cacctccagg gtaacgtcga agggggggag
     2521 cggatcagta acggctcgcg cttcaacgcc aacggcgcct acggtgcggt gaaattctgg
     2581 aaccagtggc tctcctttgg ccaggtaccg cagtggtggg gaccgggtta tgaaggaagc
     2641 ttgatccgcg gggatgcgat gcgaccgatg accggttttc tgatgcagcg agccgagcag
     2701 gcggcgccgg aaacctggtg gctgcgctgg gtgggtccat ggcagtacca gatctccgcc
     2761 agccagatga atcaatatac cgctgtgcct catgccaaaa ttattggcgg acgttttacc
     2821 ttcacgccgt tccagtcatt agaattaggt gcgtcgcgta ttatgcagtg ggggggggag
     2881 gggcgaccgc aatccttcag cagtttctgg gatggcttca ctggccatga caataccgga
     2941 acggataacg aacctggtaa ccagctggcc ggatttgact ttaagtttaa actcgagcca
     3001 actctcggct ggccggtgag cttctacgga cagatgatcg gcgaagatga gtcaggttat
     3061 ctgccttcgg ccaatatgta tctgggcggt gtggaaggcc accacagctg gggtaaggat
     3121 gctgttaact ggtacctgga agcacatgac acgcgcacca atatgagccg aaccaattac
     3181 agttatacgc atcatatcta taaggatggc tactatcagc agggttatcc gctgggtgac
     3241 gcaatgggcg gggatggtca actcgttgcc ggtaaagttg agcttattac ggaggataac
     3301 cagcgctgga gcacgcgttt agtatacgcg aaggttaacc cggagaatca gtcgatcaac
     3361 aaagcgttcc ctcatgcgga tactctaaaa ggtgtacagc tgggctggag cggcgatgtt
     3421 tatcagtcgg tccgcttgaa tacttcactg tggtacacca acgctaacaa cagcgacagc
     3481 gatgacgttg gggccagcgc agggatagag ataccattta gtttataagg ctgaatgctg
     3541 aaaacagggg agagtaggtg gtagcgctgc aggacgcagt cagcttttct ctcttcataa
     3601 gccggcaggg aagcaacggc gatgacagct gctcaggaat tggcaaattt tgatttactg
     3661 atgatgtgac attatgaaga aaaaaattgt tagattttcg gcattagcat tggcgattgg
     3721 ttttttatcg ggttgtacgg ttatcccagg ccaagggtta aatagtctgc ggaagaacgt
     3781 ggttgagctt cctgatagcg actacgatct ggacaaactg gtgaacgtgt atccgatgac
     3841 tccaggactg atcgatcagc tccgtccgga aaccgttctt gctcgtccaa atccccagtt
     3901 agataattta ctgcgcagtt atgaatatcg cattggagtt ggcgatgtgt tgatggttac
     3961 ggtatgggac cacccggaac tgacgactcc tgcaggtcaa taccgtagcg ccagcgacac
     4021 cggtaactgg gttaactctg acggtaccat cttctatcct tatatcggta aagtacaggt
     4081 ggcagggaaa acacttagcc aggtacgcca ggatattgcc agccgtttga caacatacat
     4141 cgaaagtcca caggttgacg ttagtgttgc agcattccgt tcgcaaaaag cctatgtgac
     4201 tggcgaggta gcaaaatcag gacagcagcc aattactaat atccccctta cggttatgga
     4261 tgccattaat aatgctggtg gactggctcc tgatgccgac tggcggaatg ttgtgttaac
     4321 acataatggt aaagacacaa aagtctcttt atatgcatta atgcagaaag gtgacttgac
     4381 tcaaaatcac ttactttatc ccggcgatat cctttttgta ccacgtaatg atgatctaaa
     4441 agtatttgtc atgggtgagg tgggcaaaca aagtacttta aaaatggacc gtagtggtat
     4501 gacggtagct gaagctttag gtaatgccga aggaatgtct caagcattca gtgacgcaac
     4561 aggaatattt gtcattcgtc agttaaaaga tgataagcag ggtaaaattg ctaatattta
     4621 tcagctcaac gcccaagacg cttctgctat ggtacttggt acagaattcc agttacagcc
     4681 atacgatata gtatatgtca caactgctcc tttggtacgt tggaatcgtg tcatctcgca
     4741 gcttgtaccg actataactg gcgtgcatga tatgacagaa actgggaaat tcataaggac
     4801 ttggtaataa ctatgtttaa ttcaatcctt gttgtttgta ccggtaatat ttgtcgctca
     4861 ccattaggcg aaagatattt aagagcagcc ttgcctgaaa aaaaaataga ttctgctgga
     4921 acaggggcat tagttggtta tcaggcagat ccaacagcaa ttaaaattgc tttagagcat
     4981 gatatatcgc tcgaaggaca tatagccaaa caatttactt ccacaatgtc tagacattac
     5041 gatttgattt tagtaatgga gaaatatcat atagaaaaaa taggtcgaat agctcctgaa
     5101 gctagaggca aaacaatgct gttcggt
//
LOCUS       DAPHJE010000207         5107 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPHJE010000207 DAPHJE010000000
VERSION     DAPHJE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.209"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            100..657
                     /locus_tag="RH222_004315"
     CDS             100..657
                     /locus_tag="RH222_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV3799233.1"
                     /translation="MSTTVKPSADAAAIHNTREAVRSQPGLGNLTFQMKARSSGGLTV
                     RTETGATIQNGVIDTSRVGKFSNIGDEPAGLLGTDTGMSPTEYIMQALAGCYTATLTM
                     MAAEKGIDLDGIELDLNFDINLNGFLGLDSNVRKGAKSIRVDVHLTSKTASREELEAL
                     VSEMQKNSPIHDTLANPVEMITRLA"
     gene            799..1380
                     /locus_tag="RH222_004316"
     CDS             799..1380
                     /locus_tag="RH222_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV3799234.1"
                     /translation="MTTMTRERLLSEAEHLMREKGYSAFSYADLSKIVGITKASIHHH
                     FPTKDILGEQVVIQAFSDTQRVFEQIEATEKSAEKRIAAYIDIFAQSHKASLLPLCCA
                     LSAETANLPQAITVQTSLYFDMQIEWLTKVVRAGMESGEFSSHAEPSDIALMIINVCE
                     GSSVVAHATARPEVFANSLKYIKLLLNTPHSGE"
     gene            1385..1723
                     /locus_tag="RH222_004317"
     CDS             1385..1723
                     /locus_tag="RH222_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV3799235.1"
                     /translation="MLDWNNYRSELMQRLGELGKLTPETMKGVVALGNAGNKTDLLGA
                     KVRELIALACAVTTRCDGCIAFHADAAVKAGATDAEIAEALGVAINLNAGAAVIYSAR
                     TLDAVGQARG"
     gene            complement(1753..2082)
                     /locus_tag="RH222_004318"
     CDS             complement(1753..2082)
                     /locus_tag="RH222_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDV3799236.1"
                     /translation="MRTLTAYNEDNFRQIEQYKGISVVRFSAPWCPPCQASEEMFSQF
                     ADRLDGDIQVGKVNVDQAPVLTTKYEIWGLPSVLIFHEGQLVKRIPGVKPASVYAQAI
                     ADIKKGQ"
     gene            2295..3401
                     /locus_tag="RH222_004319"
     CDS             2295..3401
                     /locus_tag="RH222_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HDV3799237.1"
                     /translation="MSKLFTPYNLSGLALKNRVVMAPMTRTRTMNDVPDEVVALYYAQ
                     RASAGLLITEGMPVSEEGRGYLYTPGIYNDEHVQGWRKVTQAVHAKGGRIFAQLWHVG
                     RMSHVSLQPGHIAPVSAGTVQAVNTTVFALTESGEPGPVVPSQPRALETHEVKRITAD
                     FVHSARLAMEAGFDGVEIMAANGFIFDQFLSSELNTRTDEYGGSVENRQRFLLETIDA
                     VAEAVGNSHVAVRLSPFGRIYDLAPYEGEEQTWSAITDALGQRELAYVHLYYQPVYTK
                     APLPEGFRRRFRNTFKGTIIAAGGFTRDIAEQALEDDELDLVAFGVPYIANPDLVERM
                     QNGWPLAESDRATYYGVSGSPEKGYTDYPVWQAQ"
     gene            3467..4168
                     /locus_tag="RH222_004320"
     CDS             3467..4168
                     /locus_tag="RH222_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDV3799238.1"
                     /translation="MQTLKPTLTIDIWSDLVCPWCWIAKKRFEQGLNRFEFRDQVVIR
                     HHSYRLAGGTPAMPFKDAIVKKLGSQHSAELMMNQVGTAGKSEGLIYNFDGMMFGDTE
                     DAHTLLVAARKAGIADAVEERFYHGSITEGRSLFDRQQLVAMAVEAGMEKADAEAALE
                     NDDFRATVSDDEAHAQSIGLSGVPVFVMNEKYAISGAQGADNFLNALRQVWDEQQTEF
                     SATAGQTCGTDGCSI"
     gene            4234..5007
                     /locus_tag="RH222_004321"
     CDS             4234..5007
                     /locus_tag="RH222_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3799239.1"
                     /translation="MNTFFKGKKLLVVGGTSGMGLAVARMVLEAGGSVVLTGNKKDKA
                     EAVQKELSPLGPVAVIAANLMTEEGMETIRHEINASHRDISLMVNAAGIFMPKGFTEH
                     SFADYDMYFSLNRATFFITQDVVKNMLAAKLEGSIVNVGSIGAQAALGDSPSSAYSMA
                     KAGLHALTRNLAIELASAGIRVNAVSPGIVHTPIYEGFMDKADIADAMKSLNDFHPLG
                     RVGTAEDVANTILFLLSDKTSWVTGAIWDVDAGIMAVRR"
BASE COUNT         1261 a         1337 c         1340 g         1169 t
ORIGIN      
        1 agagtagaca aaagaggttc gcgacattaa cctaccaact agatggttgg aactgattac
       61 accagctata acttttatca agaaattaca ggagccatta tgtctactac cgttaaacct
      121 tctgcagacg cagctgcgat ccacaatacc cgtgaagcag ttcgcagcca gcctggcctg
      181 ggtaacctga ctttccagat gaaagcccgt tccagcggcg gattaaccgt tcgcacagag
      241 accggcgcca caatccagaa cggcgttatc gataccagtc gcgtggggaa attttctaat
      301 atcggtgatg aacccgcagg tttactcggc accgataccg gcatgagccc gactgaatac
      361 atcatgcagg cgctggctgg ctgttatacc gccaccctca ccatgatggc tgctgaaaaa
      421 gggatcgacc tggacggtat cgagctggat cttaatttcg atatcaacct caacggcttc
      481 cttgggctgg acagcaacgt gcgtaaaggt gcgaaatcta ttcgcgtcga tgttcacctt
      541 acgagcaaga ccgccagtcg cgaggagtta gaagcgctgg tcagcgaaat gcaaaagaat
      601 tcgccaatcc acgatactct ggctaatccg gttgaaatga ttacacgcct ggcgtgatgt
      661 cagagatgta atgctgccat tcagaatgca ggatggcagt gacgttcagc tctgcacgat
      721 actgagtcag tgatacaact tgctacggaa aaatagcaat aatataatct atcaactagt
      781 agattatatg gagtaattat gacaaccatg acacgggaac ggctgctcag cgaagcagaa
      841 cacctgatgc gcgaaaaagg gtattcagca ttcagctatg ctgatttatc aaaaatagtg
      901 ggtatcacca aggccagtat tcatcaccat ttcccgacca aagatattct gggtgagcag
      961 gtggtaatac aggcgttctc tgatacgcaa cgcgtatttg agcagataga ggctactgag
     1021 aaaagcgcgg agaaaagaat tgcggcctac atcgatatct tcgcgcaaag ccataaagct
     1081 tcactactgc cactgtgttg tgcgttgtca gcagagaccg ccaatctgcc tcaggcaatt
     1141 actgtacaga catcacttta tttcgatatg caaatcgaat ggctcacaaa agtcgtcagg
     1201 gcgggcatgg agtcaggtga gttttcatcc catgccgaac catcagatat tgctttgatg
     1261 atcattaatg tctgtgaagg ctcaagcgta gtggcgcatg caacggccag acccgaagtc
     1321 ttcgccaaca gccttaagta tataaaactg cttcttaata cccctcattc aggagaatga
     1381 cactatgctg gactggaaca actatcgctc agagttaatg caacgtttag gcgagttggg
     1441 caaactgacc cccgagacca tgaaaggcgt ggttgccctg ggcaacgcag gtaacaaaac
     1501 tgaccttttg ggcgcaaaag tgcgtgaact cattgccctg gcgtgcgcag tcacaacccg
     1561 ctgcgacggc tgtattgctt ttcacgccga cgccgctgtg aaagccggtg caaccgacgc
     1621 tgaaattgct gaagcgctgg gcgtggcgat taacctcaac gctggcgcgg cggtcattta
     1681 ttcagcccgc actctggatg ccgtcggtca ggcacgcggt taagcattaa taaattccac
     1741 gttaaaaggg cattactgcc ctttttttat gtccgcaatc gcctgagcat ataccgatgc
     1801 aggtttcaca cccggtatgc gtttaactaa ttgcccttca tgaaatatca gcacagacgg
     1861 cagcccccag atctcgtatt tggttgtcag caccggcgcc tgatctacat tgactttccc
     1921 cacctgaata tctccatcga gccggtctgc aaactgactg aacatttctt cgctggcctg
     1981 acagggcgga caccagggtg cagagaaacg taccaccgat attcctttgt actgctctat
     2041 ctggcggaaa ttatcttcgt tataagctgt taatgtacgc atggtgatga cctcgcaaaa
     2101 tgagtgacaa ttcagaaatg gctgcgttat gacaaaaaaa tacggcacac ccgtttacct
     2161 acctactaga tggttggtat attgccatcc atcagatgtt tatgcaactc cagtgcgcga
     2221 cgtccggtcg atgatacttc cgggttgtgc aaatccctgc cgggatactc ctttttgttt
     2281 ctgtgaggta tacgatgagt aagcttttca ccccctacaa tctgtctggc ctggcgttaa
     2341 aaaaccgtgt agttatggca ccgatgaccc gcacccgcac catgaatgac gtgccggatg
     2401 aggtcgtggc tttgtactat gcacagcgtg cttctgctgg cctgctcatc accgaaggga
     2461 tgccggtttc agaagaaggc cggggttacc tctatacccc tggtatctac aacgacgaac
     2521 acgtccaggg ctggcgtaag gtgacacaag cggttcacgc caaaggtggc cgtattttcg
     2581 cgcagctctg gcacgtcggc cgtatgtctc acgtctctct tcagccaggc cacatagcgc
     2641 cggtttcagc gggcaccgtt caggcggtca ataccaccgt ttttgcgctg accgaatccg
     2701 gagaaccggg cccggttgta ccaagccagc cacgcgcgct ggaaacgcat gaagtgaaac
     2761 gcatcactgc agacttcgtg cactccgcac gcctggcgat ggaagccggc tttgacggtg
     2821 tggaaatcat ggctgcaaac ggattcatct ttgaccagtt cctcagtagc gaactgaaca
     2881 cccgcaccga cgaatacggc ggctcggtgg aaaatcgtca gcgtttcctg ctggagacca
     2941 ttgacgccgt ggcggaagcc gtgggtaaca gccacgttgc cgtgcgcctg tcaccgttcg
     3001 gccgcattta tgacctcgcg ccgtatgaag gtgaagagca aacctggtca gccatcaccg
     3061 acgcgctcgg tcagcgggag ctggcttacg tacaccttta ctatcagccg gtgtacacca
     3121 aagcgccact tccggaagga ttccgccgcc gttttcgcaa cacgtttaaa ggcaccatca
     3181 tcgctgccgg cggtttcacc cgtgatatcg cagagcaggc tctggaagat gatgagctgg
     3241 atctggtggc atttggtgtg ccctacatcg ctaacccgga tctggttgaa agaatgcaaa
     3301 acggctggcc actggcagaa agtgaccgcg ccacctacta cggcgtcagt ggttcaccgg
     3361 aaaaaggcta taccgattac ccggtctggc aggcgcaata aatcccatac ggtcgcggtt
     3421 ctctgataag tgagacccga ccgcccaagg tctttgagga atcattatgc aaacgctgaa
     3481 acccaccctc accatcgata tctggtcaga tttggtctgc ccgtggtgct ggattgctaa
     3541 gaaaagattt gaacagggtc tgaatcgctt tgaatttcgc gatcaggtgg tgatccgtca
     3601 tcacagctac cgtctggccg gtggtactcc cgctatgcct tttaaggatg ccatcgttaa
     3661 aaaactgggc agtcagcatt cagcggagct gatgatgaat caggtgggca ccgccggtaa
     3721 atctgaaggt ctgatctaca actttgacgg catgatgttt ggtgatacag aagatgcaca
     3781 caccctgctg gttgccgcgc gtaaggccgg aattgcggac gcggtggaag aacgttttta
     3841 tcacggcagt atcaccgaag gtcgctctct ctttgaccgt cagcagctcg ttgcaatggc
     3901 cgtagaagcc ggtatggaga aagctgacgc cgaagccgct ctggaaaatg acgattttcg
     3961 cgccaccgtt tccgacgatg aagcccacgc acagtctatt ggcctcagcg gcgttccggt
     4021 ttttgtgatg aacgagaaat acgccatcag cggggctcag ggggcagaca actttttgaa
     4081 tgccctgcgt caggtctggg atgaacagca aaccgaattt tcagccactg cgggtcagac
     4141 ctgcggaacg gatggctgca gcatttaatc cgtcaaccgc cggcagtttc acgctgccgg
     4201 caacgtctta cccaccagta atggagtctg aaaatgaata catttttcaa aggtaaaaaa
     4261 ctgcttgtcg tcggcggcac cagcggcatg ggtctcgccg tggcacgcat ggtactggaa
     4321 gcgggcggca gtgtggtgct gaccgggaat aaaaaagaca aagccgaagc ggtacaaaaa
     4381 gagctgagcc cgctgggacc ggtggcggtc atcgcggcca acctgatgac tgaagaaggt
     4441 atggaaacta tccgtcatga aatcaatgcc agccatcgcg atatcagcct gatggtaaat
     4501 gccgccggga tctttatgcc gaaagggttt accgagcaca gctttgccga ttatgacatg
     4561 tacttttcgc tgaaccgcgc cacgttcttt attacgcagg atgtcgtgaa gaacatgctc
     4621 gccgcgaagc tcgaagggtc tatcgtcaac gtcggttcga ttggtgccca ggcagcactg
     4681 ggtgactcgc cgtcctcggc ctattcaatg gcaaaagcgg gtctgcatgc cctgacgcgt
     4741 aatctggcga ttgaactggc cagcgcgggt attcgtgtca acgctgtgtc tcccgggatt
     4801 gttcatacac caatatacga aggcttcatg gacaaagcgg atatcgctga tgctatgaag
     4861 tcgttgaacg atttccaccc gttaggccgc gtcggaaccg cagaggatgt ggcaaacacc
     4921 attctcttcc tgttgtctga caaaacatca tgggtgacgg gcgctatctg ggatgtcgat
     4981 gccggcatta tggcggtcag acgataaccc cggtacgttg acaagagccg cccctttacg
     5041 ggcgtcattt ttttgtcgcc agaaatgtca tcagcaggct tcactcagga tattcacaac
     5101 ctgttca
//
LOCUS       DAPHJE010000208         4929 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPHJE010000208 DAPHJE010000000
VERSION     DAPHJE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.210"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..215
                     /locus_tag="RH222_004322"
     CDS             <1..215
                     /locus_tag="RH222_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3799240.1"
                     /translation="AASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIAS
                     VARTVLRAVRDSVVSVARAVWNWIFF"
     gene            433..2829
                     /locus_tag="RH222_004323"
     CDS             433..2829
                     /locus_tag="RH222_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDV3799241.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            2826..3731
                     /locus_tag="RH222_004324"
     CDS             2826..3731
                     /locus_tag="RH222_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDV3799242.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQSETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            3728..4798
                     /locus_tag="RH222_004325"
     CDS             3728..4798
                     /locus_tag="RH222_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDV3799243.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSEGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
BASE COUNT         1254 a         1197 c         1395 g         1083 t
ORIGIN      
        1 atgccgccag tcccctgatg acccgcctgc aggacgagct gcgcacggag tctgtccggt
       61 ctcaggcccg tgaacagttt accggtgcgg tggaccggat atttgacacg gcggagagcg
      121 tctgcattgc ctctgttgca cgcacggttc tgcgcgccgt ccgtgactcg gtggtctctg
      181 ttgcccgcgc ggtatggaac tggattttct tctgaacccg gtggtgatgt actaggctgt
      241 tgttacagat ttttaactcc atgaaaacac tatgcaaacg cagccatacc cgggttgtcc
      301 ccttgcgttg tgtttctcaa attaatagaa tacatcgatc gttactatca tatcgatcgt
      361 tataaacgat cgtttgtagt aaatgacctc ctccgtggtt gtcatgaaaa tgcgtcttag
      421 gaaggttaaa taatgcacga aaagaacatc gccctgcttt gtgatgaagc cgaccgactt
      481 ttgcaactga acattaatct gctccggcaa atggttgagg agccagatgt gttatctgac
      541 agtaagaacg aaaacagact gctttttgat aaacagaaag cactgaaaag aattgaggag
      601 ctggagggcg aacaaatcaa aaccgcccgc agggagatgg tgctggctgt tgtcggcacg
      661 atgaaagcag gcaaatcaac caccatcaac gccattgtgg ggcaggaaat tctgcctaac
      721 cgtaaccgcc ccatgacctc tgtaccgacg ctcatccgcc acgttcccgg aaaaactgag
      781 ccggttctcc atctggaaca tattcagcct gtccgcaatt tattaatcac actgcaggaa
      841 aaactcgcca ccccggcagg acagcaggtc gcacagaccc tgcagcaaac cggggatacc
      901 cgcgaactgc tggatattct gacggatgat ggctggctca aaaatgaata ccacggggag
      961 gaggaaatct ttaccggact ggcatcgtta aacgatctgg ttcgtcttgc tgcggcaatg
     1021 gggactgaat ttccttttga tgaatacgca gaagtgcaga aactgccggt gatcgacgtg
     1081 gaattcagcc atctggtggg gatggatgca tgccagggaa cactcacact gctggatacc
     1141 cccggcccta atgaggccgg acaaccgcag atggaagtga tgatgcggga tcaactgcag
     1201 aaagcctctg cggttctggc tgtgatggat tacacccaga tgaactcaaa agcggatgaa
     1261 gacgtccgta aagagcttaa tgccattgct gacgtatcag ccggccgcct gtttgtactg
     1321 gtcaataaat ttgatgagaa agaccgcaat ggcgatgggg cagatgccgt acgccagaaa
     1381 gttccggcaa tgctgaacag cgatgtgctg cccgcctccc gcgtttatcc cggatcctca
     1441 cgccaggcat acctggctaa ccgtgcgctt catgagttac ggaaaaacgg aacccttcct
     1501 gttgatgaag cctgggtcga tgattttatc agaggtgcct tcggttgcat gaaaaaagaa
     1561 tacgtctgta aagacagtga actggcaact gaaggggcga cagacctgtg ggaaggctca
     1621 cttatcgatc aactgataac ggaagtcata cagagctcac attccagagc tgcggcactg
     1681 gcggttgact ctgccgccgc aaaactgatg cagaatgcag aaaatatcag tgaatacctg
     1741 ttgttacgcc atcaggggct acagcaaagc attcagtcac tgcagtcgca tatcaccagc
     1801 ctgcttgcgg atatccggga aatcgcggac tgtcaggagc agatgaccac tgatgtcaga
     1861 atggccatgg aggagatcga tactaaaacc cgggaattac tgacgggggt ctgcacctca
     1921 ctggaagagg agctgaatga ctatttcaga agcggtaaac gcaaagaaca gcaaatgctg
     1981 gaggaagaag acgcagaaca acgaaggtct cagtccggat tatgggggaa aatttctcaa
     2041 tggtccggta tcaacaacca gggccgggag gattacagga aacgagattt tgccccggac
     2101 agccccgaaa taaaattcag tgatcgcagg gaagcccttg aactgatgac gcaaatcgaa
     2161 tcgaccgtga ccagcctgca ccgtgaggct gaagcacagt tccggcctga gctggagaaa
     2221 atcgtcagcg ggattgaaac aggttttcgt ggcacggccc tgtacgccac agaaaacatt
     2281 gccggtcgca tcaatgcccg cctggaggat gagggcttta ccgtaaaaat cagttttccg
     2341 gcagtcagcc agttacagac ccggctcgcg gtaaaaataa atctgagtgc gcttatggag
     2401 gaaagaacgg agaccgtcac ccgtcgccgt cggcagagtg gcgtatgggg aaccgtttgt
     2461 cgatggtttg gcaccagtga cctgggctgg gaaaactatg acgaggatgt gagtcgcagc
     2521 gtgatcaata tcaacaaggt cagagaggaa gttatgtcac tgacccgggc atatttcggg
     2581 gagctgcagg catccattga gcaggatatt aaccagcccg tccgccagga aatcgatgcc
     2641 tttttctgcg cattcaggga gaaagttgaa caactgcgta acacgctgat tcagagctct
     2701 gaagatcata aacgcgatca gcaggcgcag gaacggctta ccgggcgact tcaggcatta
     2761 aatgaaaggg ttcctgagct cattactgac agtaaggcgc tgagggaaga gctggagaca
     2821 atgctgtgac ttcaccattt attcaacaaa ttgctgataa tcgggtatgt caggtactca
     2881 cctgtcttcc tgaaaaattt gtggttgatt tcgcaaacgg aatcgatgtt gcacaggagc
     2941 atatccgcac ggccggagag cgcacgttct tccggcgttt aaaagagggg ctgaccggcg
     3001 aaggcgctgc acggcagaat gccatcaatg cgtcgcttgc acagggcgtt gaggcgtccc
     3061 tgcgctggct gacggagatg acaacgtcac ttgccaccac aaactacgca attacccggg
     3121 taaacgacag ggtcagttca ctggtcagtg atacagccag gctggcgcat tattcggctg
     3181 acacgcggga gcagttactc accctggccg atcaggttca ccataaactg aatcatcttg
     3241 aagaaaaact ccaccgtgtt gaccaggttc agcgggcgca gttacatctt gagcagatat
     3301 tctcatggtg gagcgccggg cgatatgcgt cattttcccc tgccggacgt tgttatgtgg
     3361 cacttgaaga gcttcgctgg ggcgcgtttg gtgatgtgat acgtcagagc gaaacaggcc
     3421 aggttaacca gctactggat attctcagac ataaagcatt aacgcagatg gcacaggaga
     3481 gtggcggtag tgcaaccgtg cgtctgaaca ctctggactg gcttggtggt cagggccggg
     3541 agcaggcaga taacgagtgg catgacgcga ttaactggct gggagactgg tgcagtgaag
     3601 agcagcatcc ggtgatctgg tcaaccacgc aggctgcaga acacttaccg gttcgcatgc
     3661 ctcgcctctg ctctgcagaa cgcctctctg aaagtatggt cgatgaaata tttcagaagg
     3721 gggctgcatg agtacagaaa tgaaaacggg gctggtgctg tccggtggcg gggcggtggg
     3781 cgcttatcag gcgggagtgg ttaaggcact ggcagagtgt ggtacacaga tcagcatggt
     3841 ttcaggggcc agcattggcg cattcaatgg tgccattatc gcggcctctc ccgatctgtc
     3901 agaagctgcc gtacgcctgg aggcgctctg ggatcatctg gggaataatc aggtgctgtc
     3961 ggtaaacaga ttggtttact tttcattgct gaaaaaattg ttccaggcaa tgaacctctg
     4021 ccagatcccc ggacgtgcag gagcactgct tacgacgctt cttcgccata tatcgacaat
     4081 caacgggttt gacaatctga tggctcagcc gttgttgtca gatgagcccc tgacagcgct
     4141 gatggatcat tatcttgata ctgatgctct ggcagacggg ctaccgctgt atgtgtcgct
     4201 gtaccccaca gaagggggca tgcaggatat tattgactgc attcgtgctg aactgggtgt
     4261 cggaaccacg aaaaacgccg tttttcagca tatccagagc ctgccccgcg gacagcagaa
     4321 agaggctctg cttgcgtcag ccgcgctgcc cctgctgttc cgtccccgtg aggttcaggg
     4381 gacaatgttc ggtgatggtg gtatgggagg atggcgaaat atgcagggaa atacccctgt
     4441 gacgcctctg gtcgatgccg gatgcaatat ggtgattgtg acgcatctga gtgagggttc
     4501 tttatgggat cgccaggctt ttccggacac cacaatcctt gagatccgtc cccggaaaag
     4561 gctgaaatat gcaggtgatg gtggcaacag cggcggtctg ctcagtttta catcggcaca
     4621 taccgacgcc tggcgtcagc agggctatga agacacgatg ctggcgatgg agcatatccg
     4681 gaaaccgctg gcagcacgtc aggcactgac ccggtcagag gcggtattgc agaaaagcct
     4741 ggatataacg gaagaggcag atttggcact gagaaacgcg atggcccgga ttaaataaag
     4801 acgccccaaa gaaacgccac cgcctgtggt ggtgtttttc ctgtctggtg ttattgaaaa
     4861 tattttcata tatatattac ctgagagaaa tttttacccc tcacctgtca attcactcac
     4921 tggaaattt
//
LOCUS       DAPHJE010000209         4795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPHJE010000209 DAPHJE010000000
VERSION     DAPHJE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.211"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            119..1093
                     /gene="hemB"
                     /locus_tag="RH222_004326"
     CDS             119..1093
                     /gene="hemB"
                     /locus_tag="RH222_004326"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV3799244.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDNY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1199..2050)
                     /gene="tauD"
                     /locus_tag="RH222_004327"
     CDS             complement(1199..2050)
                     /gene="tauD"
                     /locus_tag="RH222_004327"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV3799245.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2047..2874)
                     /gene="tauC"
                     /locus_tag="RH222_004328"
     CDS             complement(2047..2874)
                     /gene="tauC"
                     /locus_tag="RH222_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV3799246.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2871..3638)
                     /gene="tauB"
                     /locus_tag="RH222_004329"
     CDS             complement(2871..3638)
                     /gene="tauB"
                     /locus_tag="RH222_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV3799247.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLSLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3651..4613)
                     /gene="tauA"
                     /locus_tag="RH222_004330"
     CDS             complement(3651..4613)
                     /gene="tauA"
                     /locus_tag="RH222_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3799248.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1098 a         1388 c         1236 g         1073 t
ORIGIN      
        1 agatgtcctg gtctctcttt cattctcttc ttgtcaacat cacgacaact ttcgtaaaac
       61 actcctgccc cgcttcaggt atactgtgca ccttaattcc acaaacatca ggcagaccat
      121 gacagactta atccaacgcc ctcgtcgcct gcgcaaatct cctgcgctgc gcgctatgtt
      181 tgaagagaca acacttagcc ttaacgacct ggtgttgccg atctttgttg aagaagaaat
      241 tgacaactac aaagctgttg aagccatgcc aggcgtgatg cgcattccag agaaacatct
      301 ggcacgcgaa attgaacgca tcgccaacgc cggtatccgt tccgtaatga cttttggcat
      361 ctctcaccat accgatgaaa ccggcagcga cgcctggcgg gaagatggac tggtggcgcg
      421 tatgtcgcgc atctgcaagc agactgtgcc agaaatgatc gtcatgtcag acacttgttt
      481 ctgtgaatac acttctcacg gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga
      541 cgcgactctg gaaaatttag gcaagcaagc cgtggttgca gctgctgcag gcgcagactt
      601 catcgcccct tccgccgcga tggacggcca ggtacaggcg attcgtcagg cgctggacgc
      661 tgcaggcttt aaagatacgg cgattatgtc gtattcgacc aagttcgcct cttcctttta
      721 tggcccgttc cgtgaagctg ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat
      781 gaacccaatg aaccgtcgcg aggcgattcg tgagtcactg ctggatgaag cccagggcgc
      841 agactgcctg atggttaaac ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga
      901 acgtactgaa ttgccgattg gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt
      961 cgccgcgctg gcgggtgcta tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat
     1021 taagcgtgcg ggtgcggatc tgattttcag ctactttgcg ctggatttgg ctgagaagaa
     1081 gattctgcgt taattttttt cctctcagaa agggggagtt aacctgttga aagtacggtg
     1141 aacgccccct caccctaacc ctctccccaa gggggcgagg gaaccgtccg cactcgtatc
     1201 accccgcccg ataaaacggt ttatccccaa gaatcgtcgc ccgatgcata atccgccgct
     1261 gtggcaggta atcggcattg gcatagtgct gagtcacgcg gttatcccaa atcgcaatat
     1321 cattcggttg ccagcgccaa cgcacctgaa actccggttt agtgatatgg gcgaataaaa
     1381 agcctaacaa ggcttcgctc tctttctcgc tgacatcgac aattcgcgta gtaaagcctt
     1441 cattcacaaa cagcgcctgt ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca
     1501 acggaggatt tttcgcgacc gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt
     1561 atttgtattc cgggaacgat ttacggaaat catgctccgc acgcagcccg ctcagcagct
     1621 ggcggaaggg aacagagagc gcctcatagg ccgcaatacc gctggtccag agcgtatcac
     1681 cgccggtcga aggtaattct ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa
     1741 atgtcacatc ggtatgccag ttgtcgttat ctggcggatt atcgttatgg gtatccagca
     1801 cgatgatctc gtcaacccct tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa
     1861 aacgctgggc cagcgcgcgt tgctgctgcg gcgtaatggc ttgatcgcgc agaaacacca
     1921 cctgatggcg cagcaccgca tggtaaagct gttcaaactg attatcgctt aacgggcgcg
     1981 tcaggtcggc acccgaaatt tgtgcgccga tatacggccc cagcggggta atgctcagac
     2041 gttcactcat tgtacttctc catgccaggg cgtcagacgg cgctgtaacg cgcgcaaacc
     2101 cagttctaaa agaaaggcga taatcgcaat caccgcgatt cccgccagca ccacgtcagt
     2161 cgcgagaaat tcacccgctg actgaaccat aaatcctaaa ccgcgcgtcg cggcaatcag
     2221 ctccgccgct accagcgtag accagcccac acccagacca atgcgtaatc cggtgaggat
     2281 ttccggcagc gcgccgggca aaatgacaaa ccacagcacc tgcgcacggc tggcacccag
     2341 cgactgggcg gcacgaatgc gaacctgctg cgcgcttttc acccccgcca gcgccgacat
     2401 cgccaccggt gcaaaaatcg ctaaatagat cagtaagatc ttcgaggttt caccaatacc
     2461 aaaccagatc accatcagcg gcaaataagc cagcggcggc accggacgat aaagttcgat
     2521 taccggatcc agaatgccgc gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat
     2581 accgatcaac accgccgcga gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg
     2641 ctgccacagc gtggcgtcca taaatccctg cggcccggca atggtgagca gttttgccag
     2701 tacctgttgc ggtggcggca aaaacagcgg gctaatcagt tgcagcgctg cgactgccca
     2761 ccatacagta agtaaaaccg ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg
     2821 ccagcgccat ttaagccgat gcgaatgcag tttttcatta atgagcacac tcatgagaac
     2881 gcctcccgct gctcaaatac gcggcttaaa acatattcgc gcatggcgat gaattgtgga
     2941 tcggacttga tgctgcggct cgactctccc gcaacaaagc ggcgagcaaa gttgagcgac
     3001 agccgctcca gcacacggcc agggccggat gaaagcagaa ccagttcagt cgccataaac
     3061 accgcttctt ctatatcgtg ggtaatcaac agcacctgct tgcccgtctc ctgccagagt
     3121 ttcagcagca gggtttgcat ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg
     3181 tcgagtaata acagctgggg attcgccgcc agcgcacggg caatccccac ccgctgacgt
     3241 tgaccaccgg aaagctgcca gatgtagcgt ttttctgcgc cttccagccc cacttttttc
     3301 agcatctggt gcgcgatttc cagtcgctgc attttctcta tacctgccaa ttgcaggccg
     3361 aacgccacgt tgtcctgtac attgcgccac ggtagtagcc cttcattctg aaaaactacg
     3421 ccacgctctg ctcccggtcc ctcaatacgc ttacccgcca gtagaatgct gccatgctga
     3481 taaggcacaa aaccggcaat cagattcagc aacgtggttt taccgcagcc ggacggcccc
     3541 agcaccacca gtagctcgcc gctttccagc gtcaggttga tatcttccag tgccggtttg
     3601 ccgccataat cggcgtaaag atgagagatt tgcagcatct gcgcctcctt ttattgcacg
     3661 aagcgcgagg taacgtactg gctgtaatca ttcgctacag ccgggacctt gccctgctct
     3721 ttcaaaaact gcgcggtgtc gatgatcgct ttgttcaccg gtccggtcag ttctgccgtt
     3781 tgttgctgcg gcgtcagata ggtattcccc ttcaccagcc ccggaacgtc accttcaggc
     3841 acgccgctta aacgcgccag tttgctgatg ttttccggct gtttcagcca cacgtctggg
     3901 ttagcaatgt acggttgctg agcatcgatg gcgcttttag cgaacgcttt cacgacctca
     3961 ggatgtttct cggcaaaatc tttgcgcacc acccagacgt ccagcgttgg cgcgccccac
     4021 tgcccgacct gttcagaatc ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc
     4081 ggtgcccaga cataagcacc atcaatatct ccccgctgcc aggcagcgat aatcgcgggc
     4141 ggctgcaggt tcacaatctc cacttgcccg ggtttaatgc cccagtgttt cagcgccgcc
     4201 agcaggctgt agtgggtggt ggagataaac ggtacggcga tgcgtttgcc aatcagatct
     4261 tccggtttgc tgatagtttt ctttaccacc agcgcttcgg agttacccag ttttgacgcc
     4321 agcaagaaga cttcaatcgg cacctgttgg ctggctgcaa ccgctaacgg gctggaaccg
     4381 aggttgccga tttgcacatc gcctgaagcc agcgcccgca cgatgctggc tccgctgtca
     4441 aacttacgcc agtccacgct cgccccgctc tctttagcaa aggtgttgtc ggcctgagcc
     4501 actttcgccg gttcggctga ggtttgatag gccacggtga cgttcaccgc ctgtgcctga
     4561 aaagcgatga atgccagtgc ggctagaagt gtgttacgcg atgaaattgc catgattgtc
     4621 tgctcccctg tcttgttatg ggagcagtat tcaggaataa aaacattcat taaaagaatt
     4681 agtcgttatc gcacagatga ttttattctt agcaaaaaaa cggctaagga aagattaaat
     4741 atgcaattac tccgttctaa caattgggaa aattactaca cttcatttta gtgat
//
LOCUS       DAPHJE010000210         4730 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPHJE010000210 DAPHJE010000000
VERSION     DAPHJE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.212"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..282
                     /locus_tag="RH222_004331"
     CDS             <1..282
                     /locus_tag="RH222_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HDV3799249.1"
                     /translation="VNEWFSAYAMAGVAYSRISTFSGDYLRVTDNKGKTHEVLTGSDD
                     GRHSNTSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            347..4126
                     /locus_tag="RH222_004332"
     CDS             347..4126
                     /locus_tag="RH222_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HDV3799250.1"
                     /translation="MAVKISGVLKDGAGKPVQNCTIQLKAKRNSTTVVANTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVACNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDASASKVAAKSSETSAASSAGSAASSATAAGNSAKAAKT
                     SETNADNSAQAAADSQTASANSATAAKKSETNAKNSESAAKVSETNAKASENKAKEYL
                     DKVGGLVSPMTQYDWPVVTASESLYIKIAKLSDPGTSRSHVTLMVTNAGNYGSPYGNI
                     DFIEISARGLPSLLSADNVSRHLSIRRLGSTGLTDNNQMRYGLVKGDGFIEVWAFQGA
                     FINDAKVAVLAQTTLNTELYIPDGFVKQTAAPSGYIEGNVVRIYDQVNKPTKADLGLS
                     NAMLTGAFGLGGSGISTNGKMSDVEILKALRDKGGHFWRGDKPPGSTATIYSHGSGIF
                     SRCGDTWSAINIDYSTAKIKIYAGNDARLNNGTFSVNELYGSANKPSKSDVGLGNVTN
                     DAQVKKTGDTMTGDLTIKKGTPSVFLRADSGVTALRFYTGDNTERGIIYAGPNTDSLG
                     EVRIRAKTAGGASGGDLVVRHDGRVEVRDLTVAYKIKSRTIEIANTDTDSSATTLSIY
                     GVQHTPLVLTRSGSSENVSIGFKLDNMNPKYLGIDTNGDLAFGESPDQKQNSKLITQA
                     KLDKGLTIGGQLAFKGTTAFSAVATFSAGIAGAIEPENIDGQTVNLNNLTIIKSDAGA
                     VKYYICPSSAGGANITNKPDGVTGNFLLRVESTRKVRDSDYANMQTLINSDTKRIYVR
                     FVVNGNWTAWSQVVVSGWNQDVTVRSLTSTTPSKLGGGRIDVLGSTSDYGSMNCTVRG
                     VDSTGTNSAWSVGTSESTGKMLFLKNHRSSAQVLLNGDDGAVQLLSGTVNGATAQALT
                     INKDEVNSTADLVIRKQTGTGNRFALLNSGNSELPVSIRVWGSSTRQNVFEVGTSAAY
                     LFYAQKTTDGQNLTVNGSVNCTTLNQSSDRRLKENIEIIDNATDAIRKINGYTYTLKE
                     NGAHCAGVIAQEVEEAIPEAVGSFIHYGEELQGPTVDGNELREETRYLNVDYAAVTGL
                     LVQVARETDDRVTALEEENTTLRQNLATAGTRITTLENQVSELVALVRQLTGSEH"
     gene            4178..4309
                     /locus_tag="RH222_004333"
                     /pseudo
     CDS             4178..4309
                     /locus_tag="RH222_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            4643..>4730
                     /locus_tag="RH222_004334"
     CDS             4643..>4730
                     /locus_tag="RH222_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3799251.1"
                     /translation="MTGHGFRHTMSTILHEEGFNSACFETQLA"
BASE COUNT         1302 a         1098 c         1273 g         1057 t
ORIGIN      
        1 gtgaatgaat ggttcagcgc gtatgcgatg gcgggtgtgg cttacagccg tatttctact
       61 ttctccgggg attatctccg cgtaactgac aacaagggga aaacgcacga agtgctgacc
      121 ggaagtgatg atggtcgcca cagcaacacg tctctggcgt ggggggctgg cgtgcagttt
      181 aacccgaccg aatccgtggc cattgatatt gcttatgaag gctccggcag tggcgactgg
      241 cgcactgacg gtttcatcgt gggtgtcggt tataaattct gattagccag gtaacacagt
      301 gttatgacag cccgccggtt caggcgggct tttttgtggg gtgaatatgg cagtaaagat
      361 ttcaggtgta ctgaaagacg gcgcaggtaa accggtacag aactgcacaa tccagctgaa
      421 agcaaaacgt aacagcacca cggtggtggc gaacacggtg gcctcagaaa atccggatga
      481 agccgggcgt tacagcatgg acgttgagta cggtcagtac agcgttattc tgttggtgga
      541 aggcttcccg ccatcgcatg ccgggaccat caccgtgtat gaagactcac aaccgggtac
      601 gctgaatgat tttctcggtg ccatgacgga ggatgatgtc cgtccggagg cactgcgccg
      661 ctttgaactg atggtggaag aggtggcgtg taacgcgtcc gcagtggcgc agaacacagc
      721 agccgcgaag aagtcagcca gcgatgccag cacatcagcc cgtgaggcgg caacccatgc
      781 gactgatgct gcaggctcag cacgtgcagc cagcacgtca gccggacagg ccgcttcgtc
      841 ggctcagtca gcgtcttcca gcgcaggaac ggcatcagca aaggccactg aagcatcaaa
      901 aagtgctgcc gctgcagagt cttcaaaaag cgcggcagcc accagtgccg gtgcagcgaa
      961 aacgtcagaa acgaatgccg cagcatcaca aaaatctgcg gccacttctg catccaccgc
     1021 gaccacgaaa gcgtcagaag ctgccacctc agcccgggat gcgtcggctt caaaagtggc
     1081 ggcaaaatca tcagaaacga gcgcagcctc gagcgctggc agtgcagctt cctcggcaac
     1141 ggcggcagga aattccgcga aggccgcaaa aacgtctgag acgaatgcgg ataacagcgc
     1201 acaggcggca gcagactcac aaactgcatc ggcaaattcc gcgacagcag ccaaaaaatc
     1261 agaaaccaac gcgaaaaata gtgagtcagc agcaaaggtc agcgaaacca acgctaaagc
     1321 gtcagagaac aaggcgaaag aatatctcga caaggtcggg ggactcgtca gcccgatgac
     1381 gcaatacgat tggcctgttg ttactgctag tgagtctctt tacatcaaga tcgcgaaact
     1441 ttccgatcct ggaaccagca gaagtcatgt aacgctaatg gttactaacg ctggtaacta
     1501 cggctcccct tacggaaaca ttgactttat cgagatctcg gcgcgcggtc tgccttcttt
     1561 gcttagtgcg gataatgttt ctcgtcatct gagtatacgc cgcttagggt caaccgggct
     1621 gaccgataac aaccagatgc gttacggcct ggttaaaggt gacggcttta ttgaggtttg
     1681 ggcattccag ggtgcgttta ttaacgacgc aaaggttgcg gtgctggcgc agacaacact
     1741 aaacacagaa ttatacattc cagacggatt tgttaagcaa accgccgcgc cttctggata
     1801 tattgaaggc aacgttgtaa ggatttacga ccaggtaaac aagccgacta aagcagattt
     1861 gggtctttct aatgctatgc ttacaggcgc tttcggtctt ggcggtagcg ggatatcaac
     1921 aaacggcaag atgagcgatg tagagatctt aaaagctctg cgtgacaaag gtggtcattt
     1981 ctggcgcggt gataagccgc ccggaagcac ggcgaccatt tatagccacg gttctggtat
     2041 attctcgcgg tgcggcgata cgtggtcagc gatcaatatc gactactcaa ccgcgaagat
     2101 taagatctat gccggcaacg atgcccggct taacaacggg acttttagcg tcaatgagct
     2161 atacggctcg gcaaacaagc cgtcgaaatc ggatgttgga cttggcaacg taacgaacga
     2221 tgcgcaggta aaaaaaaccg gcgatacaat gaccggtgac ttgacaatca aaaaaggtac
     2281 accgtcagtc ttcctgcggg cagacagtgg agtcaccgct ttgcggtttt atactggcga
     2341 taacacagag cgcggcataa tctatgctgg tcctaacact gattcgcttg gcgaagttcg
     2401 catcagggca aagacagcag ggggggcatc aggaggggat cttgttgttc gtcacgacgg
     2461 gagggttgaa gtccgtgatc tcacagtagc gtataaaatt aaaagcagaa cgattgagat
     2521 tgcaaatacc gacactgact catcggcaac tacgctaagc atctatggag tacagcacac
     2581 gccgttggtt ttaacgcgtt ctggttcttc tgaaaatgtg tccattgggt ttaagttaga
     2641 caacatgaac ccaaagtatc ttggaattga tactaatggg gatctggctt ttggtgagag
     2701 tcctgatcag aaacaaaaca gcaaattgat cacgcaagcg aaactcgaca agggattaac
     2761 gattggtggt caactggctt tcaaaggtac gacagcgttt tcagccgttg ctacgttcag
     2821 tgccgggata gcaggagcca tcgagccgga aaacattgac ggccagacgg ttaatcttaa
     2881 caacctgacc atcatcaagt cagatgccgg ggcagttaaa tactatattt gtccatcctc
     2941 tgcaggtggt gcaaatatca ccaacaagcc tgacggcgta acaggtaact ttttgctccg
     3001 tgttgaatcg actcgtaagg ttagggattc agattatgcg aacatgcaaa cgctgattaa
     3061 cagcgacaca aaacgtatat acgttcgctt tgttgttaat ggaaactgga cagcgtggag
     3121 tcaggttgtt gtttccggat ggaatcagga tgtaaccgtc aggtcgttaa cctcgacgac
     3181 tccatcaaaa ttaggtggcg ggcggattga tgtgcttgga agtacgtcag attatggcag
     3241 catgaattgt accgttcgtg gtgttgatag cactggaacc aattcggcgt ggtcggtagg
     3301 cacatcagaa agcacaggca aaatgttgtt cctgaaaaac cacagaagca gcgctcaagt
     3361 gctgttaaat ggcgatgatg gagcggttca actactaagt ggcactgtta acggtgctac
     3421 agcgcaggcg ctaaccatca acaaagatga ggttaactca actgccgatt tagtaattag
     3481 aaaacaaaca gggactggca atcgttttgc tttacttaat tcaggtaatt cagaactacc
     3541 agttagtatc agggtgtggg gttccagtac tcgacaaaac gtttttgagg ttggcacgtc
     3601 tgctgcgtat cttttttatg cgcaaaaaac aacagacggg caaaacctta ctgtaaacgg
     3661 aagtgttaac tgcaccacac tgaatcagtc atcagaccgc agactgaaag aaaatatcga
     3721 gattatcgat aacgcgactg acgcaatccg caaaattaat ggatacacgt acacgctcaa
     3781 ggagaatggg gcacattgcg ccggggttat agcccaggaa gttgaagagg ccatcccgga
     3841 ggccgttggt tcattcatcc attacggtga agagttgcaa ggcccgaccg ttgacggcaa
     3901 cgagctacgc gaagaaacgc gctatcttaa tgttgactac gccgccgtga cgggtttact
     3961 tgttcaggtc gcccgtgaaa cagatgatcg cgttaccgcg ctggaagagg aaaacacaac
     4021 gctacgtcaa aatctggcaa cagcaggcac ccggatcacc actctggaaa atcaggtaag
     4081 cgaactggtt gcacttgtcc ggcagttaac aggaagcgaa cattgatatc cttcaagccc
     4141 tgaaggaggc tgttcctggt acgttcagac tgttgttgag ctggaaatcg caacggagga
     4201 agaaacctca ttgctggaag cctggaaaaa gtatcgggtg ttgctgaacc gtgttgatac
     4261 atcaactgca cctgatattg agtggcctac gaaccctgtc agggagtaat cattgggatt
     4321 atgccgcaga aacgttgtat gcaataacgt tctgcggttg gctgatgcac tttcgagagt
     4381 gcatgtattg aattatttcc agctattatc gattttacgt attttttgca tgagagaatt
     4441 tgtacctcct cccaccgatc ctccatgact ttacgccact gtctctagga ctgctatgtg
     4501 ccaggaacgg acatagaaaa cacgatgtta taagattagg tgcatctatc cgatagtatt
     4561 cattggacgt gatgatcgta ctaagccgat ctcaaaggaa agcattaatc aggtaattga
     4621 gctattagga taaaaaggaa ggctgacggg gcatggtttt cgtcacacaa tgagtaccat
     4681 tctgcacgaa gaaggattca actctgcatg tttcgaaaca caacttgcac
//
LOCUS       DAPHJE010000211         4657 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPHJE010000211 DAPHJE010000000
VERSION     DAPHJE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.213"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(99..533)
                     /gene="merR"
                     /locus_tag="RH222_004335"
     CDS             complement(99..533)
                     /gene="merR"
                     /locus_tag="RH222_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HDV3799252.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            605..955
                     /locus_tag="RH222_004336"
     CDS             605..955
                     /locus_tag="RH222_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HDV3799253.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            969..1244
                     /gene="merP"
                     /locus_tag="RH222_004337"
     CDS             969..1244
                     /gene="merP"
                     /locus_tag="RH222_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HDV3799254.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1280..1702
                     /gene="merC"
                     /locus_tag="RH222_004338"
     CDS             1280..1702
                     /gene="merC"
                     /locus_tag="RH222_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HDV3799255.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1754..3448
                     /locus_tag="RH222_004339"
     CDS             1754..3448
                     /locus_tag="RH222_004339"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HDV3799256.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3466..3828
                     /gene="merD"
                     /locus_tag="RH222_004340"
     CDS             3466..3828
                     /gene="merD"
                     /locus_tag="RH222_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HDV3799257.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3825..4061
                     /gene="merE"
                     /locus_tag="RH222_004341"
     CDS             3825..4061
                     /gene="merE"
                     /locus_tag="RH222_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HDV3799258.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            4097..>4657
                     /locus_tag="RH222_004342"
     CDS             4097..>4657
                     /locus_tag="RH222_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3799259.1"
                     /translation="MAQAAARGQLDLHYQPLVDLRDHRIAGAEALMRWRHPRLGLLPP
                     GQFLPLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSASQVGPTFDDEV
                     KRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDFGTGYSCLQHL
                     KCCPITTLKIDQSFVARLPDDARDQTI"
BASE COUNT          824 a         1508 c         1478 g          847 t
ORIGIN      
        1 atggcagcgc ctacggcgct gactctttcg ttgaccaggc gcaagcgcct ggaaatgctc
       61 aattaggggg cacctcagaa aacggaaaat aaagcacgct aaggcatagc tgaccttgcc
      121 aggcctgctt cgccctgtag tgacgcgatc aacgggcagg aaacattccc ctttcgtgca
      181 tggcaggcgc acacgagttc agacagcacg gtttccatgc gcgccaagtc ggccatcttc
      241 tcgcgcacgt ccttgagctt gtgttcggcc aggctgctgg cctcctcgca gtgggtgcca
      301 tcgtcgagcc gcaacagctc ggcaatctcg tccagactga accccagccg ctgtgccgat
      361 ttcacgaatt tcacccgaac cacgtccgcc tccccatagc ggcggatgct gccgtaaggc
      421 ttgtccggtt cccgcaacag gcccttgcgc tgatagaagc ggattgtctc cacgttgacc
      481 ccggccgcct tggcaaaaac gccaatggtc aggttttcca aattattttc catatcgctt
      541 gactccgtac atgagtacgg aagtaaggtt acgctatcca atccaaattc aaaagggcca
      601 acgtatgtct gaaccacaaa acgggcgcgg tgcgctcttc gccggcgggc tggccgccat
      661 tcttgcatcg acctgctgcc tggggccgct agtactggtc gccctgggct tctccggtgc
      721 ttggatcggc aacctgacgg tgctggaacc ctatcgaccg ttgttcatcg gcgcggcgct
      781 agtggcgctg ttcttcgcct ggaagcggat ttaccggccc gtgcaggcat gcaagccagg
      841 tgaggtctgc gcgattccgc aggtgcgcgc cacctacaag ctgattttct ggatcgtggc
      901 cgtgctggtc ctggtcgcgc ttggatttcc ctatgtcgtt ccatttttct attaaccagg
      961 agttcatcat gaagaaactg tttgcctccc ttgccctcgc cgccgctgtt gccccggtgt
     1021 gggccgctac ccagaccgtc acgctagcgg ttcccggcat gacttgcgcc gcctgcccga
     1081 tcacagtcaa gaaagcgctc tccaaggtcg aaggcgtgag caaggtcgat gtgggcttcg
     1141 agaagcgcga ggccgtcgtc acttttgacg acaccaaggc cagtgtacag aagctgacca
     1201 aggccaccgc agacgccggc tatccgtcca gcgtcaagca gtgagccagc aagccaacga
     1261 caacagcgag agccgcttca tgggactgat gacacgcatt gccgataaaa ccggcgcgct
     1321 cggcagcgtc gtttccgcga tgggctgcgc cgcctgcttt ccagccctcg ccagcttcgg
     1381 cgcggccatc gggctgggct tcttgagcca gtacgaggga ctgttcatca gccgcctgct
     1441 gccgctgttt gccgcgctgg ccttcctggc gaacgcgctg ggttggttca gtcatcggca
     1501 atggctgcgc agtctgctcg gcatgatcgg cccggccatc gtgtttgcgg ccacggtctg
     1561 gctgctcggc aactggtgga cggcgaacct gatgtacgtc ggcctggcct tgatgattgg
     1621 ggtgtcgatc tgggacttcg tgtcgccggc gcatcgccgt tgcggaccgg acggctgcga
     1681 actccccgcc aagcgcttgt gaaagacggc tgaccgtgcg acacggcggc ccacacgaat
     1741 aaggaacgat ggtatgagca ctctcaaaat caccggcatg acttgcgact cgtgcgcagt
     1801 gcatgtcaag gacgccctgg agaaagtgcc cggcgtgcaa tcagcggatg tctcctacgc
     1861 caagggcagc gccaagctcg ccattgaggt cggcacgtca cccgacgcgc tgacggccgc
     1921 tgtagctgga ctcggttatc gggccacgct ggccgatgcc ccctcagttt cgacgccggg
     1981 cggattgctc gacaagatgc gcgatctgct gggcagaaac gacaagacgg gtagcagcgg
     2041 cgcattgcat atcgccgtca tcggcagcgg cggggccgcg atggcagcgg cgctgaaggc
     2101 cgtcgagcaa ggcgcacgtg tcacgctgat cgagcgcggc accatcggcg gcacctgcgt
     2161 caatgtcggt tgtgtgccgt ccaagatcat gatccgcgcc gcccatatcg cccatctgcg
     2221 ccgggaaagc ccgttcgatg gcggcatcgc cgctaccacg ccgaccatcc agcgcacggc
     2281 gctgctggcc cagcagcagg cccgcgtcga tgaactgcgc cacgccaagt acgaaggcat
     2341 cttggagggc aatccggcga tcactgtgct gcacggctcc gcccgcttta aggacaatcg
     2401 caacctgatc gtgcaactca acgacggcgg cgagcgcgtg gtggcattcg accgctgcct
     2461 gatcgccacc ggcgcgagcc cggccgtgcc gccgattccc ggcctgaaag acactccgta
     2521 ctggacttcc actgaagcgc tggtcagcga gacgattcct aagcgcctgg ccgtgattgg
     2581 ctcatcagtg gtggcgctgg agctggcgca ggcgttcgcc cgactcggag cgaaggtgac
     2641 gatcctggct cgcagcacgc tgttcttccg cgaagaccca gctataggcg aagccgtcac
     2701 ggccgcattc cgcatggagg gcatcgaggt gagggaacac acccaggcca gccaggtcgc
     2761 gtatatcaat ggtgaagggg acggcgaatt cgtgctcacc acggcgcacg gcgaactgcg
     2821 cgccgacaag ctgctggtcg ccaccggccg cgcgcccaac acacgcaagc tggcactgga
     2881 tgcgacgggc gtcacgctca ccccgcaagg cgctatcgtc atcgaccccg gcatgcgtac
     2941 aagcgtggaa cacatctacg ccgcaggcga ctgcaccgac cagccgcagt tcgtctatgt
     3001 ggcggcagcg gccggcactc gcgccgcgat caacatgacc ggcggtgacg cggccctgaa
     3061 cctgaccgcg atgccggccg tggtgttcac cgacccgcaa gtggcgaccg taggctacag
     3121 cgaggcggaa gcgcaccatg acggcatcaa aactgatagt cgcacgctaa cgctggacaa
     3181 cgtgccgcgc gcgctcgcca acttcgacac gcgcggcttc atcaaactgg tggttgaaga
     3241 aggcagcgga cgactgatcg gcgtgcaggc agtggccccg gaagcgggcg aactgatcca
     3301 gacggccgca ctggcgattc gcaaccggat gacggtgcag gaactggccg accagttgtt
     3361 cccctacctg acgatggtcg aagggttgaa gctcgcggcg cagaccttca acaaggatgt
     3421 gaagcagctt tcctgctgcg ccgggtgagg acaaggaggt gtgcgatgag cgcctacacg
     3481 gtatcgcaac tggcccataa cgctggggtg agcgtacata tcgtgcgcga ctacctggtg
     3541 cgcggcttgt tacggccggt ggcctgcacc acgggcggct acggcgtgtt cgacgatgcg
     3601 gccttgcaac ggctgtgctt cgtgcgcgcg gccttcgagg cgggtatcgg cctggatgcc
     3661 ctggcgcggc tgtgccgtgc gctcgacgca gcggacggcg cacaagccgc agcgcagctt
     3721 gccgtgctgc gccagttggt cgagcggcgg cgcgcggcgt tggcccatct ggacgcgcaa
     3781 ctggcctcca tgccagccga gcgggcgcac gaggaggcat tgccgtgaac gcccctgaca
     3841 aactgccgcc cgagacgcgc caacccgttt ccggctacct gtggggtgcg ctggccgtgt
     3901 tgacctgccc ctgccatctg ccgattctcg ccgccgtgct ggccgggacg accgccggtg
     3961 ccttccttgg cgagcattgg ggtgttgccg cgctcgcgct gaccggcttg ttcgttctgg
     4021 ccgtaacgcg gctgctgcgc gccttccggg gcggatcatg acgagttcgc agcccgccgg
     4081 atggacggcg gccgagttgg cgcaggcggc ggcgcgcgga cagcttgacc tgcattacca
     4141 gccgctggtc gatctgcgcg atcaccggat cgctggcgcg gaagcgttga tgcgctggcg
     4201 gcatccgagg cttggcctgt tgccgcccgg ccagttcctg ccgctggccg agtcgttcgg
     4261 cctgatgccg gaaataggcg cgtgggtgct gggcgaggcc tgtcgccaga tgcacaagtg
     4321 gcaaggaccg gcatggcaac cgttccgtct tgccatcaat gtgtccgcca gccaggttgg
     4381 gccaacgttc gacgacgagg taaagcgggt gctggccgat atggccctgc ccgccgagct
     4441 tctggagatc gaactgaccg aatcggtcgc attcggcaat ccagccctgt tcgccagttt
     4501 cgacgccttg cgcgccatcg gcgtgcgctt cgccgccgac gacttcggca ccggctattc
     4561 ctgcctgcaa catctgaaat gctgccccat caccacattg aaaatcgacc aatcctttgt
     4621 cgccaggctc ccggatgatg cccgtgacca aactatc
//
LOCUS       DAPHJE010000212         4637 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPHJE010000212 DAPHJE010000000
VERSION     DAPHJE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.214"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..107
                     /locus_tag="RH222_004343"
     CDS             <1..107
                     /locus_tag="RH222_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV3799260.1"
                     /translation="DRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            111..1466
                     /locus_tag="RH222_004344"
     CDS             111..1466
                     /locus_tag="RH222_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HDV3799261.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            1514..2554
                     /gene="gatD"
                     /locus_tag="RH222_004345"
     CDS             1514..2554
                     /gene="gatD"
                     /locus_tag="RH222_004345"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV3799262.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            2660..3433
                     /locus_tag="RH222_004346"
     CDS             2660..3433
                     /locus_tag="RH222_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3799263.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(3515..4414)
                     /gene="yegS"
                     /locus_tag="RH222_004347"
     CDS             complement(3515..4414)
                     /gene="yegS"
                     /locus_tag="RH222_004347"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV3799264.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1126 a         1035 c         1216 g         1260 t
ORIGIN      
        1 tggatcgtag ttttggcgat attccgttag ttcacggcat gccttttatt tctggtgtcg
       61 gtatcgaagc attacaaaat aaaattctga ctatcttaca ggggtgacct atgttttcag
      121 aagtcatgcg ttatattctc gacctcggcc ctacggtgat gcttccgatt gtcatcatta
      181 ttttttctaa aatattaggc atgaaggcag gcgattgctt taaagcgggt ctgcatatcg
      241 ggattggctt tgttggcatt ggccttgtga ttggcttaat gctggattcc attggtccgg
      301 cggcgaaagc gatggcggaa aatttcgacc tgaatctgca tgtggtcgat gtcggctggc
      361 cgggctcttc accaatgacc tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga
      421 ttctggttaa cgtggcgatg ttactgaccc gtatgacgcg ggtggtaaat gttgatatct
      481 ggaatatctg gcatatgacc ttcaccggcg cgttgctgca tctggcaacc ggttcatgga
      541 tgatagggat ggcaggtgtg gtaattcacg cggcgtttgt ttataagctc ggcgactggt
      601 ttgcccgcga tacccgaaat ttctttgagc tggaaggtat tgctattccg cacggtacgt
      661 cggcgtatat ggggccgatt gcggtgctgg tcgatgctat catcgagaaa atcccaggcg
      721 ttaaccgaat taaatttagc gccgacgata ttcagcgcaa atttggtcca tttggcgagc
      781 ctgtcaccgt gggttttgtg atggggctga ttatcggcat cctcgcgggt tacgatgtca
      841 aaggtgtatt gcagctggcg gtaaaaacgg cggcagtgat gctgctaatg ccacgggtga
      901 ttaaacccat catggatggt ttaacgccca tcgctaagca ggctcgtagt cgtttacagg
      961 cgaagttcgg cggtcaggag ttcctgattg gccttgatcc ggcgttgctg ctgggacata
     1021 cggcggtggt atcggcaagc ctgattttta tcccactcac cattttaatt gctgtttgtg
     1081 tgccgggtaa tcaggtgctg ccgtttggcg atcttgccac catcggcttc ttcgtggcga
     1141 tggcggtcgc cgtgcatcgt ggaaatctgt tccgcacctt aatctcgggt gtcatcatta
     1201 tgagcatcac cctgtggatc gcgacgcaaa ctattggttt gcacacccaa ctggcggcta
     1261 atgctggggc gttaaaagcc gggggtatgg tggcttcaat ggatcagggc ggttctccca
     1321 ttacctggtt actgattcag gttttctccc cgcaaaatat tcccggtttc attattatcg
     1381 gtgcaattta tctgaccggt attttcatga cctggcgtag agcgcgtggc tttattaaac
     1441 aagagaaagt cgttctcgca gaataatttt tacctgaggg ggaggttatc ccccttaaca
     1501 atcaggagtt tttatgaaat cagtggtgaa tgatactgat ggtatcgtgc gcgttgcaga
     1561 aagcgtcatt cctgaaatta aacatcagga tgaggtgcgg gtaaaaattg ccagctcggg
     1621 cttatgtggt tccgatttac ccaggatatt taaaaatggt gcacattatt atccaataac
     1681 gttaggccat gaatttagcg gctatattga tgcggtggga tccggtgttg atgatttaca
     1741 ccctggcgat gcggttgcct gtgtgccgtt attaccctgt tttacttgtc cagagtgtct
     1801 gaaagggttt tattcccagt gcgcaaaata tgattttatt ggctcgcggc gtgatggtgg
     1861 atttgctgaa tatattgtcg ttaagcgaaa aaatgtcttt gctctaccca cggatatgcc
     1921 tattgaggat ggggctttta ttgagccgat taccgttggt ctgcatgctt ttcatttagc
     1981 gcaaggttgt gagaataaaa acgttattat tattggtgcc ggaaccattg gcctgctggc
     2041 tattcagtgc gctgtcgcgc tgggagcaaa gagtgtgacg gctatcgaca ttagctcaga
     2101 aaaactggca ctggcaaaat ctttcggtgc gatgcaaaca tttaacagta gcgaaatgag
     2161 cgcgccgcaa atgcagagcg ttttacgcga actgcgtttt aatcagctta tcctcgagac
     2221 ggctggcgta ccgcaaactg tcgaactggc ggtagagatt gccggtcctc atgcccaact
     2281 ggcgctggtg ggcacgttgc atcaggatct gcatttaaca tcggcaacgt ttggcaaaat
     2341 attgcgtaaa gagctgacgg ttatcggcag ttggatgaac tattccagcc cttggccggg
     2401 gcaggagtgg gaaacggcga gccggttgct gacagaacgt aagttaagcc tggagccatt
     2461 aatcgctcac cgtggaagct ttgaaagctt cacccaggcg gtgcgtgaca tcgctcgtaa
     2521 tgccatgccg ggcaaagtgt tgctcattcc atgaaaccgc gggccagcgt gatgctggcc
     2581 cggtattgtg caaaacagat cattcaccaa tggtccccct tcgtttacac tagccacaat
     2641 tgaatatggg taaatgacga tgaattcatt cgagcgaagg aataagatca tccaattagt
     2701 gaatgaacag ggaaccgtgc ttgttcagga tctggcggga gtatttgctg cctcggaagc
     2761 gacaatccgt gccgatttgc gctttctcga acaaaaaggc gtggttacgc gctttcatgg
     2821 cggtgcggcg aaaataatgt ccggtaatag tgaaaccgag acccaggaag tcgggtttaa
     2881 agagcgattt cagctcgcca gcgcgccaaa aaacagaata gcgcaggcgg cagtcaaaat
     2941 gatccatgaa gggatgaccg ttattctcga cagcggaagt accacaatgc ttatcgccga
     3001 aggattaatg accgctaaaa atatcacggt gattaccaac agtctcccgg cagcgtttgc
     3061 cctttccgaa aataaagaca ttactctggt cgtctgtggt ggcacggtcc gccataaaac
     3121 gcgctcgatg catggttcca ttgccgagcg ttcattgcag gatattaatg ccgatttaat
     3181 gtttgtcggt gctgatggta ttgatgcggt taatggtatt acgaccttta atgaaggtta
     3241 ttcgattagc ggtgcgatgg tcaccgccgc taataaagtg attgccgttc tcgattcatc
     3301 gaaatttaac cgtcgcggtt ttaatcaggt actgccaatc gaaaaaattg acattattat
     3361 taccgatgat gctgtatcag aggtggataa actggcattg cagaagacac gggtaaaatt
     3421 aattacggtg tagtattgtt ctgtatgccg gataaggtat ttacaccgca tccggcattt
     3481 cggattctcc ctggttcaag agagaatagt ttgattaacg caataatgga caatctggtg
     3541 gtaatcgaca acgcaacgcc gccggaagta tttcaatatg aaagttttgt ccgctcaacg
     3601 gttcgccatc aagattaaaa gtgatttcgt gtggggcttg tatatcaaac cacgacgaag
     3661 cgccttcgat aatattcggg ttatcttcgt cagattttaa ggttgatacg agagccggaa
     3721 gaatttcatc gccggtaaaa atgcgcagtt gcagcaagcc atcgttaatt aacgcgttcg
     3781 gacacaattg ctgaccgcca ccggcctgac gcccgttacc aataccaatg accagggcgt
     3841 caccttgcca gtgaaagttt tcaccgcgga tttcacaacg gtccggttgc agagtatcca
     3901 tgcgcattaa gccatgaatg atgtaagaga cgccacccag cgcggctttt aatttttccg
     3961 gcgtttctgt ggtaatacgc gtcccaaatc cgcctgtcgc catattaata aaacaggttt
     4021 gtttgttgac ctgcgccata tctatcgcaa tggcgttacc ggcaattgcc agtttcagcg
     4081 ccttatccag tgcctcagga atccctacac tggtggcaaa atcattggcg gttcctaatg
     4141 gcaaaattcc cagcgcgggt atgccatccc cctcacactg aatcaacgcc gtagaaactt
     4201 cattaatggt gccatcgcca ccaccggcaa tcaccgttgc gacgcccaac ttccgggcct
     4261 cctctacata tcgtgcggca tcgcctttct cccaggtgac ccgcacatgg atcgtcattc
     4321 cttcctcacg caacagcata attgcttcgc gcaagggtag attgtcagta cttttgccat
     4381 taagaatcag taagctggcg ggaaattctg ccatgatcgt atgtgccttt atgattggtc
     4441 tgtagatagt gtagagcaga aaacaaaagg tggagtcaga acaggatgaa agtcggagga
     4501 taaaaagtca gttcaagtat ggaggattca gtggttacca ccaggagctt gcaatgggga
     4561 cagcgaatac taacggctgc caccgataaa tttcaaaaaa gagcatatac ctaatattca
     4621 actaaacagt ggcatct
//
LOCUS       DAPHJE010000213         4599 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPHJE010000213 DAPHJE010000000
VERSION     DAPHJE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.215"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(143..778)
                     /gene="msrQ"
                     /locus_tag="RH222_004348"
     CDS             complement(143..778)
                     /gene="msrQ"
                     /locus_tag="RH222_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV3799265.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
     gene            complement(779..1783)
                     /gene="msrP"
                     /locus_tag="RH222_004349"
     CDS             complement(779..1783)
                     /gene="msrP"
                     /locus_tag="RH222_004349"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV3799266.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            complement(1892..2305)
                     /gene="hiuH"
                     /locus_tag="RH222_004350"
     CDS             complement(1892..2305)
                     /gene="hiuH"
                     /locus_tag="RH222_004350"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV3799267.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2438..3109
                     /gene="hprR"
                     /locus_tag="RH222_004351"
     CDS             2438..3109
                     /gene="hprR"
                     /locus_tag="RH222_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV3799268.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3109..4467
                     /gene="hprS"
                     /locus_tag="RH222_004352"
     CDS             3109..4467
                     /gene="hprS"
                     /locus_tag="RH222_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV3799269.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(4524..>4599)
                     /locus_tag="RH222_004353"
     CDS             complement(4524..>4599)
                     /locus_tag="RH222_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV3799270.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1295 a          972 c         1072 g         1260 t
ORIGIN      
        1 tcttattgaa atataataaa ttatgtcgat gatttgaggt tgatgacaaa cgaaacataa
       61 cttgatgtga tctgcgtagg ccggataggg cgctcgcgcc gcatccggca gggataacgc
      121 gtactttgtc ggcaatatga agttatacag ataatttgtt gtgaacctgc ttgcgtaacc
      181 ggttaaaaag ggaaagcaac ttcttatacc gtaaggctaa aagcagtacc gccagcccag
      241 cgtagatgag tggctgcggt gaaataatct tcactgacca caaataatgt attggggcca
      301 ggatcgcgac aagatagacg aagttatgca actgttgcca gtgtttaccg agttttcgct
      361 gcatcgcctg ggttgaagtg aacgctaacg caagcaaaat tacccagctg ataataccta
      421 acgttaaata aggccgggta attaactcct tacctaataa cgccagatta ttcacgccca
      481 gctccagcaa tgcgtaactg gttaaatgca gtgtcgccca ggcaaagcac cataagccta
      541 acaggcggcg agtgcgtatc aataacggct gttttgcgta gcgtgccaga ggtgtgatta
      601 acaaggtcgc cagcaaaaat ttcagtgcag tgcgaccagt aaaatgctga atatctttca
      661 ccggatcggc acccaatcca ccgtgattaa tcgcccagac cagccagaga aacggcaaca
      721 atccggcaag atgcaggcaa actttcagcc atgtcacctg ttttgctgtc agacgcattt
      781 agaaattctc ccgcaaatcc aggccacgat acagcgatgc cacctgctcg gcgtaaccat
      841 taaacagtag cgttggctgg cgctgtacat cgaggatgcc gcctgaacca ataaatcgtt
      901 cggtagcctg tgaccagcgc gggtgatcaa catgcggatt aacgttggcg taaaaaccgt
      961 attcgtcagg cgctgccaga ttccaggtgg ttggcggacg ctcgcgggtc agcttaatac
     1021 tgactatcga tttaatccct ttaaagccat atttccacgg cacaatcagt cgcaccggcg
     1081 cgccattttg tggcggtaac gccttgccat aaacacccac ggtcatcagt gtgagcggat
     1141 gcattgcttc gtcgagacgc aatccttcga cataaggata tttcagcccg ccaccgataa
     1201 agcggtcctg ctggcctggc atttgttccg gtgcataaat tgtttcgaaa gcgacatact
     1261 tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg cagcggaaaa ccaatccacg
     1321 gcaccaccat cgaccatgct tccacgcagc gcatacgata aatacgctct tccagcggaa
     1381 agcgacgggt taaatcatcg tgatcgaggg tcaatggttt tgccacttcg ccgctgattt
     1441 tcagtgtcca tggatcggtt ttcaggctac cagcattagc ggcgggatcg gctttatcca
     1501 gcccgaattc atagaagttg ttataaccgg agactttatc tgctggcgtc agtggcaggt
     1561 cattttgcca ggcggcaggc ttgctgaact ccaacggttt tccggcgggg gcgggtgggc
     1621 gatcattccc tttaaaccag ctaagcagat cggcatgcgc agcgtgaggc aaagaaagtg
     1681 cagccgcgct gatgcccagt gcttttaaca cctgccgacg cgtcataaag aataccgact
     1741 cggccgtaac atctgattct tttaaaaatt gattcttttt cataagccat cacacttttt
     1801 tgtctttagc cagcaagagc cgtttactgt taatctgtca ggaagtgtcc tccctaacag
     1861 tagcagagat ctgcttttac atatttatgc attaactgcc acgataggtt gaatacccat
     1921 attggctaag taataaaggc acatgataat gctcgttcac tttattaata tgaaattcaa
     1981 cggggatctc agggaagaaa ctttcaagat tttgtttctt gaaatagtcc ccggttttaa
     2041 atacgacacg gtaatcgccc gtagttgcag tttgctcggg ccacagtgcc ttaattcgtc
     2101 catccttatc tgttttggcg gtattaagtt gtaaccagcc gttgtccgct ttcttttcaa
     2161 gagtgactgt cacgtcggca gcgggttttc ctgtttgctg gttcaaaatg tgcacactaa
     2221 gaatgttttg ttgtgcagca tagaccaaag aaggtaatga aaatgcagcc gttactacgg
     2281 agagtactaa ataacgcttt aacatgttta tatccttgtc atgtgaatga gttttgagga
     2341 tagcgaaaaa gcgtccacgg taaccttaca gcagcattac aattttgtaa tgaaccaggc
     2401 tgtttctata acatatgatt tatggcatat tattttcatg aagattttac ttattgaaga
     2461 taatcaaagg acccaggagt gggtaacgca ggggctttcc gaagcgggtt atgtcattga
     2521 tgccgtttct gatggcagag atgggcttta tcttgcgctt aaggatgatt atgcattgat
     2581 cattctggat attatgcttc cgggtatgga tggctggcag atcttacaaa cgttaagaac
     2641 agcaaagcaa actcctgtta tttgccttac tgcaagagat tctgtcgatg acagagtcag
     2701 agggctggac agtggggcaa atgattatct ggtaaaacct ttttcatttt ctgagttgtt
     2761 ggcaagggtt cgggcacaat taaggcaaca tcacgctttg aattcaacat tagaaatcag
     2821 tggcttaaga atggactctg ttagtcaaag tgtgagcagg gacaatatca gtattacact
     2881 gacgcgcaag gagtttcagt tactttggct actggcctcc agagctggcg agattatccc
     2941 cagaacggtt attgcgagtg aaatttgggg aatcaacttt gatagtgata ccaatacggt
     3001 ggacgtcgcc attcgcaggc tccgcgcaaa agttgatgat ccttttcctg aaaagctaat
     3061 cgccacaatc cgggggatgg gctattcatt cgtagcggta aaaaaataat gaaaagatta
     3121 tctataaccg tccgtttaac cttgcttttt atattgctgc tgtctgttgc tggcgctgga
     3181 attgtatgga ctctctataa tggcctggca agtgagttga aatggcgcga tgatacaaca
     3241 ctcattaacc ggacagcgca gatcaagcag ttgttaattg atggggtaaa tccagatacg
     3301 ttacctgtgt actttaaccg gatgatggat gttagtcagg atatcttgat cattcatggt
     3361 gatagcatca acaaaattgt taaccggacg aatgtcagtg atgacatgtt aaataacata
     3421 cctgctactg agacaatcag cgcagctggc atttacagaa gcattattaa tgatacagag
     3481 atagatgctt tacgaattaa tattgatgaa gtttcgccat cattaacggt tactgtggct
     3541 aaattggctt cagccagaca taacatgctt gaacagtata aaattaatag cattataatt
     3601 tgcattgtcg ccattgtact ttgctcagta ttaagtccgc tgttaatcag aacgggatta
     3661 cgagagatca aaaagttgag tggtgtaacg gaagcgctga attataacga tagccgggag
     3721 cctgttgagg ttagcgcatt accgagagaa ctaaaacctc ttgggcaggc gttgaataaa
     3781 atgcatcatg ctttagtcaa agattttgag cgtctaagtc agtttgctga cgatctcgct
     3841 catgaactta gaacgcccat taatgcatta ctgggtcaga atcaggttac gctcagtcaa
     3901 accagaagta tcgctgaata tcaaaaaaca attgccggta acattgaaga gctggaaaat
     3961 atttcgcggt taacagagaa catactgttt cttgcccggg cagataaaaa caatgttttg
     4021 gtgaaactgg actcactttt tctcaataag gaagtcgaaa atttgttgga ttatcttgaa
     4081 tacctttcag acgagaaaga gatttgcttt aaggtcgagt gcaatcagca aatctttgcg
     4141 gataaaattt tactgcaacg aatgttatcg aatcttattg ttaatgccat tagatattca
     4201 ccagaaaaat cgcgcattca tataaccagt tttcttgata ccaacggcta tcttaatatt
     4261 gatatcgcca gtcctggaac gaaaattaat gagcctgaaa aactcttccg tagattttgg
     4321 cggggagata attcgcgtca ttccgaaggt cagggacttg gtctttcttt agtcaaagcg
     4381 attgccgaat tgcatggggg aagtgctacg tatcactatt ttaataagca taatgtgttc
     4441 cgaattacgt taccgcaaag aaattaacat agggcttcag tacgccataa atcaaccatg
     4501 aaaaaacggg taatgactcc aacttactga tagtgtttta tgttcagata atgcccgatg
     4561 accttgtcat gcagctccac cgattttgag aacgacagt
//
LOCUS       DAPHJE010000214         4509 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPHJE010000214 DAPHJE010000000
VERSION     DAPHJE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.216"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..290
                     /locus_tag="RH222_004354"
     CDS             84..290
                     /locus_tag="RH222_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3799271.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            385..987
                     /locus_tag="RH222_004355"
     CDS             385..987
                     /locus_tag="RH222_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799272.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLKHHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWTENLKRK
                     SEKELEEWIIDRQKQSAALTDLMDQYVLLAYRTTVDDNRN"
     gene            1624..2003
                     /locus_tag="RH222_004356"
                     /pseudo
     CDS             1624..2003
                     /locus_tag="RH222_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750026.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3575..4447
                     /locus_tag="RH222_004357"
     CDS             3575..4447
                     /locus_tag="RH222_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799273.1"
                     /translation="MVKVVEDERSRIRYLERRLNENGFYLPSSLADKDYFSYQKRILN
                     TLISQGADTLKINNFLAETDQRYFDSLPSEDDLNWYRNDARASLWLTCELYEMIKING
                     YENTLTCLSPESLPSHHSVRVDAIRRCIDNWPFILYTPSNYLNQKSIEWTTLLEKDDI
                     FREVKARNFDICSWLKKYIQEKTNISLNYVCGESSEEIMAWCYASYFTWKKNNQNSPD
                     SVELFTRKFKSAWATQKNRIKNRVDKKLRPLNVNISQEAYDKLRKLSINEGISNDRVI
                     ESALDMIYRSKIKK"
BASE COUNT         1356 a         1023 c         1017 g         1113 t
ORIGIN      
        1 ccccggttgt tatgccggtg aaggtggtgc gttgttttca tgaccacacc ggctggttta
       61 acaacatccg gaggaacatt ctcatgacca ccccagtttc gctgatggat gaccagatgg
      121 tcgatatggc gtttatcact cagctgaccg gcctgaccga taagtggttt tacaagctca
      181 tcaaggatgg ggcctttccg gctcccatca aactcggccg cagctcccgc tggctgaaaa
      241 gtgaagtgga agcctggcta caggcgcgta ttgcacagtc ccgtccgtaa tttctgcccc
      301 ttatccgttc acccgcagca gacgtaatcc ccggcctgcc gacggcattc tgctgcctgt
      361 tatatccccg tgaggaatat gaaaatgaaa caacagtatc agacccgcta cgaatggctc
      421 cacgaaagct accagaaatg gctgaccggc ttcacccggc acgccgtatc ctggggcgtg
      481 tgtcatccga atatctacta cttccataat ctgacgcccg gttgggtgtc attcaatggt
      541 gaaaaaccgg agattgccat cgtcccccag agcctgcacc ggctgattta tggccctgac
      601 aaacgggcca cgccgcccct ggatgatgat ctgatagtga atttatgcac cagtgaacat
      661 ctgctggttc atcatccgat gctggaaggc attctgctgt ctgaatgtga acgtctgaaa
      721 caccattctc tggcaaataa actgatcagt ctcttccgtc agtttggtgg cacggagtta
      781 cgccttaaac tggtctggct ttgctggctt gatttaatga ccggaaacag ccttgaagac
      841 tggacagaga acctgaaacg gaaatcagaa aaagagctgg aggaatggat catcgaccgt
      901 cagaagcaga gcgcagcact gacggatctg atggatcagt acgtgctcct ggcataccgc
      961 acaacggttg acgataaccg taactgatgc cggttgcttc cggaaggtgc ggcaaaatac
     1021 gggaatgatg cttccggccc cacatactca cctgcagagc gtgttcgtcg tcatcatact
     1081 gagccatgac ggatggctta agggcatcct gtcattgctg aactggcatg aacaccacca
     1141 gtgaatgaaa gccatggaca gggatgttct ttacagccag atacacgcca cgttctctac
     1201 ggactgaata tgcaggcaga ggttatttac cagaaatggc atgctgcggt acatgcctca
     1261 tcctggtgac aataagtaca tggcgacagc gctccttgca ctgcggaaga aactcacacg
     1321 gcaggatgtt atggctgaag tccccggaga tcattgtttc ttcgtcagat attcaggaac
     1381 agttgtagta acgaatttta ccggtctctg cacatggccc ggctccgtct gtgtggcaca
     1441 ggttacgggt attgccggtt gtaggaacgg acggtgagtt gagtgatctg tggaatcagg
     1501 gagcattctg ggcattatgg tgctgaaatg tgaccatcga cacaataccc ctgcttcgtg
     1561 gtacacaata accaggaaac ataccctgaa ccgacagcca taaggacccg tcacaaaatg
     1621 gtgctaatga aagcgaaaca ggaaaaccgg cttgagcgac agctccaata actctgttac
     1681 gcacgcgtac tgatccttga tgaaacaggc tatctgccaa tgccccggaa agaagccagg
     1741 ctgttcttcc ggttgctgaa gcggcaatat gaaaaagcga gaatcgttct gacgtcaaat
     1801 aaaggattcg ccaactgggg ggaaatgctc ggcgataatg tactggcaac agtgatccct
     1861 gagcatctgc tgcatcactc aaccacgctg aatattaagg ggggaaagct accacctgaa
     1921 ggaaaaacgt aaagcaggtg tactggcaag ggccagcgcc aataagtgat gatgaatcgg
     1981 tggaaaccgg gcatcgccat tgataaatag cggcaatcaa ataaaacggt gaaaaaaggt
     2041 tatatacagc ggcgttgaca atacaggagg taatactcac caataaataa gcgtcaatac
     2101 ggtgctccgt tgacgcttac cattgctcat tcagttgagc aataaattgt cccggataat
     2161 ccagtccttc acattgtctg ctaccagatg aaacttgcgc ccctatccgg ggctgtcact
     2221 ccatgcagcc agactttatg tttcccttca ccagaatacc cccagagtga taatgtcgat
     2281 aaccagatgt ggaatttcgc agagagctgg cttctgatgc tgacgctaac gtgcttttcc
     2341 cacccgctaa ccagaaagta atctgctcag catatcagta accccatcag cttaaaatca
     2401 aatcaataaa gccctgtaaa acctgaagta acagtccggg aactcgtatc atgctaacat
     2461 atcagtgttg tgaagcagtc gacaacgagc ttcagacggt acacacgtca attcatgcag
     2521 aaatagcagc cacatcaggt ctggctgtca gcggaccacg cagcctgagt acattgttgt
     2581 aaattaaacg actgatgggt tttaaatttt ttctttgcca ccaccgtgaa ctgataccgg
     2641 aaaaacagta gtctcaggaa gagtgatctg aaagtcagct ttattaaaaa agctattaga
     2701 ccgggtaaat aatgtcaacg gatgaaatgt tatgataatt ctggatgcca tatctatcag
     2761 taaatcagta acgccagagg gtatggtaat atctaagaaa tactttacgg taatcaggaa
     2821 tatttacttg ccatcatggc ttatgaaatt catggttcat gagtcactgt aactcccgta
     2881 tcgtccgtca attatagttt acgttaacga tatatctgtg tctgcaggca ctacaatgaa
     2941 ttcccgaata aatttccgac cagtcatttt ctctgacaag gaagcccata tgatttccct
     3001 gtacccgtga cagactggtg aacaatacca tatctgtaag atatgattaa tgaggaaagt
     3061 gatgaccgtt agtcatttta tctccagtaa cgtaaaacca gacttatcgt aaataaaaat
     3121 atgaagccct ctgttaaagt catggagaga cgcatcaaaa tacaggatac aatgattctc
     3181 acaatgaaac tcatcgtctc tggtgaaatt catatcaaca tttaccttaa taacctgacg
     3241 cgggattaac tgtgttgttt aaggcaaagg ggagagttgc ccgaaggcga aaacgaaagt
     3301 aaattaataa taacaggatg agaggagaaa gcgctcttcc cggaaaataa aaagagcggt
     3361 tcactcctgt gaatgcactt caaccgtcct ggcaaagaat gtacttttat aaaaccccct
     3421 cccagtcagc agatacaaaa agataacatg aggtatctcc cgccccttaa tttatacacg
     3481 tgaaatgaac tgaaatttat gagaaaatca tgctaaagat gctttgattt tacttaaaat
     3541 cacgtgtgat tatcctgact gaaggagaga tactatggtt aaggttgtgg aagatgaaag
     3601 aagcagaata cgatatcttg aaaggcgctt aaatgaaaat gggttttatt taccatcaag
     3661 cctggccgat aaagattatt tctcgtatca gaaacggatt ttaaacactc ttatttcaca
     3721 gggtgctgac acactgaaaa taaataattt tctggctgaa acagaccagc gttacttcga
     3781 ttccttacca tctgaggatg atcttaactg gtatcgtaat gatgcccgcg ccagtttatg
     3841 gttgacttgt gaattatatg aaatgattaa gataaacggt tatgaaaata cccttacctg
     3901 tttatctccg gagtcactgc cttctcacca cagcgtgagg gtggatgcca tacgcaggtg
     3961 tattgacaac tggcctttca ttttatacac accatcaaat tacctgaacc agaaaagtat
     4021 tgaatggacc acactactcg aaaaagatga tattttcaga gaggttaagg ccagaaattt
     4081 tgatatctgc tcatggctca aaaaatatat tcaggaaaag acgaacattt ccctgaacta
     4141 tgtctgtgga gaatcatctg aagagattat ggcctggtgt tatgccagtt acttcacatg
     4201 gaaaaaaaac aaccagaatt caccagactc agtagaactg tttacccgga agtttaaaag
     4261 cgcctgggct acccaaaaaa acagaataaa aaatagggtt gataaaaagc tgcgccccct
     4321 gaacgtaaat atttcacagg aggcatatga caaactgaga aaattatcca taaacgaggg
     4381 tatctcaaat gaccgggtta ttgaaagtgc tctggacatg atttaccgta gcaaaattaa
     4441 aaaataaaac cattcaaaat aatttcagac agatattatc tccgtgagtc ccccgccacc
     4501 tttccggtg
//
LOCUS       DAPHJE010000215         4429 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPHJE010000215 DAPHJE010000000
VERSION     DAPHJE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.217"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..445)
                     /locus_tag="RH222_004358"
     CDS             complement(<1..445)
                     /locus_tag="RH222_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HDV3799274.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQDNELHISWGKIGTHGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADK"
     gene            complement(586..2619)
                     /gene="metG"
                     /locus_tag="RH222_004359"
     CDS             complement(586..2619)
                     /gene="metG"
                     /locus_tag="RH222_004359"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV3799275.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2751..3860
                     /gene="apbC"
                     /locus_tag="RH222_004360"
     CDS             2751..3860
                     /gene="apbC"
                     /locus_tag="RH222_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV3799276.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGVKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4123..4404
                     /locus_tag="RH222_004361"
     CDS             4123..4404
                     /locus_tag="RH222_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV3799277.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1077 a         1101 c         1121 g         1130 t
ORIGIN      
        1 atttgtctgc ttgctgaaca ggaactccgg gaaagcggcg atgacttaac gtggtgtgta
       61 caacttcggg cggaaaaacg acagcaatat ctttcgccag ccacggtaaa acagcatctg
      121 tatttttgtc aggcgcacgg gtttctgcta cgaaagaggg ttgttcctga ataatggtgg
      181 ataagtccgc taccttgaga gagcccgtta ccgtttggga aggttgcaaa gaattatcct
      241 tcgcttgctc cacatacccc ttcttcacct tctccgcaat cagcttaagc tccgcttttg
      301 ccgctgccgc agcatctgaa aaacttttta tctgactttg tccgtgggta ccgattttgc
      361 cccagctaat atgaagctca ttatcctgtt gctcaactgc ccagaattta tgtgattttt
      421 cgtcctgata gataaagtgt ctcattctgc atccctgtaa ttttatccag ttgaatgcag
      481 atgctaccag tatttatgcg ggttagagag agacaaaatc gcaagaaaac aactttagga
      541 atttataaag aaaaggcgct gtcgatgcag cgccttaaag ggggattatt tcacctgatg
      601 gcctggttta gcaccagcat ccgggcttaa caggaagata tctttcccgc caggaccagc
      661 agccattacc atgccttcgg agataccaaa gcgcatttta cgcggtgcca ggttggccac
      721 cataatggtg tgacgcccga tcagtgcctg cggatccggg taggcagaac gaataccgga
      781 gaagacattg cgtttttcac cgccgagatc cagtgtcagg cgcagcagtt tgtcagaacc
      841 ttcaacaaac tctgcgtttt cgatcagcgc cacgcgcagg tcaacttttg cgaagtcgtc
      901 aaaggtgatg gtttcctgaa tcggatcatc tgccagtggg ccagtaactg gcgcggcagc
      961 tgctttcact tcttctttag acgcttccac cagtgcttca acctgcttca tatcgatgcg
     1021 gttatacagc gctttgaacg gattcacttt gtggcccagc agcggttgct ggataccatc
     1081 ccaggtcagt tctgtattga ggaatgcttc tgcgcgctcg gtcagtttcg gcagtaccgg
     1141 cttcaggtaa gtcatcagca cgcggaacag gtttatgccc attgagcaaa ttgcctgcag
     1201 gtcggcatcg cggccttcct gttttgccac cacccacgga gcctgctcgt cgacatagcg
     1261 gttagccaga tcagccagcg ccatgatttc gcgcactgct ttaccaaatt cgcggctttc
     1321 ccacgcttca ccaatcactt cagcggcatc agtgaaggtt ttgtacaact gcgggtcagc
     1381 cagttcgctt gccagcacgc cgtcaaaacg cttgttgata aagcccgcat tacgagaggc
     1441 caggttaacc actttgttca cgatatcggc attcacacgc tgaacgaaat cctccaggtt
     1501 gagatcgata tcatcaatgc gcgaagagag tttcgcagtg tagtagtaac gcaggctgtc
     1561 agcgtcaaaa tgattcagcc aggtgctggc tttaataaag gtgccgcgag acttggacat
     1621 ctttgcgccg ttcaccgtca catagccatg aacaaacagg ttggtcggct tgcggaagtt
     1681 gctgccttcc agcatggcag gccagaacag gctgtggaag taaacaatat ctttaccgat
     1741 gaagtggtac agctcggcgg tggagtcttt cttccagtat tcatcgaagc ttacgctgtc
     1801 gccgcgcttg tcgcacagat tcttgaaaga acccatgtag ccaatcggtg cgtccagcca
     1861 gacgtagaaa tatttgcccg gcgcgttcgg aatttcaaaa ccaaagtatg gcgcgtcgcg
     1921 ggagatatcc cactgttgca ggccggattc aaaccactcc tgcattttat ttgccacctg
     1981 ctcctgcaac gcaccgctgc gggtccatgc ctgcaacatt tcgctgaaag agggcagatc
     2041 aaagaagaag tgttcagaat cacgcattac cggcgtagcg ccagaaacca ccgatttcgg
     2101 ctcgatcaat tcagtcgggc tgtaggtcgc gccgcagact tcgcagttat cgccgtattg
     2161 atccggggat ttacatttcg ggcaggtgcc tttcacaaaa cggtccggca ggaacatgcc
     2221 tttttccgga tcgtacagct gagagatggt gcggttttta ataaaaccgt tctctttcag
     2281 gcgagagtag ataagttctg acaactggcg gttctcttcg ctgtgcgtcg agtgatagtt
     2341 gtcatagctg atgttaaagc ctgcgaaatc agtctgatgc tcctgactca tttcgccaat
     2401 catctgctcc ggggtgatac caagctgctg agctttcagc atgatcggtg taccgtgggc
     2461 atcgtcggcg cagatgaagt tgacctcgtg gccgcgcatt cgctggtaac ggacccagac
     2521 atcagcctgg atgtgctcca gcatatggcc gaggtggatt gagccgttag cgtacggcag
     2581 tgcgcacgtc accagaattt tcttcgcgac ttgagtcata gtaggcatta cttcttaata
     2641 gtgaaaaggg gttagatagt accaaatggg aaaatgttaa gtaagtactt ctctatattc
     2701 ttcaggtaaa ctatcagctg atggtttatc tatacaaaaa aggagtcggg atgaacgaac
     2761 aatcccaggc caaatcacca gaagccttgc gcgcaatggt cgccgggaca ctggccaatt
     2821 ttcagcaccc aaccctgaag cataacctca ccacgctgaa agcgttgcac catgtcgcct
     2881 ggatggacga cacgttgcat gtggaactgg ttatgccatt cgtctggcat agcgcgtttg
     2941 aagagttaaa agagcaatgt agcgccgaac tgctgcgtat caccggggtg aaagctatcg
     3001 actggaaact gtcgcacaat atcgccacct taaaacgcgt gaagaatcag ccgggtatta
     3061 atggtgtgaa gaacatcatt gcggtcagtt ccggtaaagg cggcgtgggt aaatcctcca
     3121 cggcggtaaa cctggcgctg gcactggcgg cggaaggggc gaaagtcggc attctggacg
     3181 ccgatatcta cggcccatca attcccacca tgctgggcgc agaaaatcag cgtccaactt
     3241 cgccggacgg tacccatatg gcaccgatta tgtctcatgg cctggcaacc aattcaattg
     3301 gttatctggt cactgacgac aatgcgatgg tgtggcgtgg accgatggcg agcaaggcgt
     3361 taatgcagat gttgcaggaa accttgtggc cggatctcga ctatctggtg cttgatatgc
     3421 cgccaggcac cggtgacatt caattgacgc tggcgcaaaa cattccagta actggtgcag
     3481 tcgtggtcac caccccgcag gacatcgcgt tgatcgatgc gaaaaaaggc attgtgatgt
     3541 tcgagaaagt cgaggttccg gtgcttggta ttgtcgagaa catgagtgtg catatttgca
     3601 gtaactgtgg tcaccatgaa ccgattttcg gtaccggtgg tgcagagaaa ctggctgaaa
     3661 aataccacac acagctgctg gggcagatgc cgttacacat ttccttacgt gaggatctcg
     3721 ataaaggcac gccaacggtt atcagccgtc cggagagcga atttaccgct atctaccgcc
     3781 agctggctga ccgcgttgca gctcagctct actggcaggg tgaagtcatt ccaggcgaga
     3841 tttccttccg cgcggtctaa cgcctattaa ccattctggt tattcttcat acaccgcaga
     3901 gatgttacat tgatgcggtg tattagtttt tgccgcatta atgaacactt atttaatact
     3961 gcgaatattc tgcggacctt cgtccgaaac tctacaaagt cgattgatca tacaggcaat
     4021 gcttcattat catccttcgt ggctgtgtag tggatgtaca gctatagttt gttatgcact
     4081 cattctatcc aggcataacc tttactacag ggataacgtt caatgaataa gtattggctg
     4141 tcaggaatta tttttcttgc ctatggattg gcttctcccg cattctcttc agagactgcc
     4201 acattaacga ttaatggcag gatcagtccc ccaacatgca gtatggctat ggttaatagt
     4261 caaccacaac agcattgtgg tcagttaacg tataatgttg acacccgtca tcaggtttct
     4321 tcgcccgtca aaggtgtaac gacagaagtg gttgttgcag gtagcgatag caaacgtcga
     4381 atcgtgttaa atcgctacga ttaatttggc aacctgattt taattgcat
//
LOCUS       DAPHJE010000216         4352 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPHJE010000216 DAPHJE010000000
VERSION     DAPHJE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.218"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..558
                     /gene="fimA"
                     /locus_tag="RH222_004362"
     CDS             16..558
                     /gene="fimA"
                     /locus_tag="RH222_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3799278.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            778..1470
                     /gene="fimC"
                     /locus_tag="RH222_004363"
     CDS             778..1470
                     /gene="fimC"
                     /locus_tag="RH222_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV3799279.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1501..4109
                     /locus_tag="RH222_004364"
                     /pseudo
     CDS             1501..4109
                     /locus_tag="RH222_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            4122..>4352
                     /locus_tag="RH222_004365"
     CDS             4122..>4352
                     /locus_tag="RH222_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799280.1"
                     /translation="MKIICRLLLAMACLCLENISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNY"
BASE COUNT         1158 a          948 c         1031 g         1215 t
ORIGIN      
        1 attgaaggaa tatatatgaa attaagattt atttcgtctg cgctggctgc cgcactattc
       61 gccgctacgg gtagttatgc tgccgttgta gatggcggta caattcactt tgaaggcgaa
      121 ctggtgaatg ctgcctgttc agtgaatact gactcggcag accaggttgt cacactcggt
      181 caatatcgta ccgatatttt caatgctgtt ggtaatacct ctgcattaat tccattcacc
      241 attcagttga acgactgcga tcctgttgtt gccgctaatg ctgccgttgc attttctggt
      301 caggctgatg caatcaatga taatttattg gccattgcat ccagtaccaa tacaacaaca
      361 gcaacgggtg tcggtattga aatacttgat aatacatccg caattctcaa acctgatggg
      421 aatagcttct caaccaacca gaacttgatc cccgggacca acgttcttca tttttctgca
      481 cgttataaag gcaccggtac aagtgcatca gcagggcaag caaatgctga cgcgactttt
      541 attatgagat atgaataatc aaaaccacgt tgttttgaat tatatatcac gtcttataac
      601 aaagtaatgt accggttgtc tgaagcggta tggtggcaat gtaaatcgaa atcatgttca
      661 ctttgtatca tgccgcttta ttaaatgaaa agggaatgat gtgttgtaag aaaccaaagc
      721 aatcatttct ttatattcct tatttttgcc gtcaggaata cacaaggcgt attaactatg
      781 atgactaaaa taaagttatt gatgctcatt atattttatt taatcatttc ggccagcgcc
      841 catgctgccg gagggatcgc attaggtgcc acgcgtatta tttatcccgc tgatgctaaa
      901 cagactgcgg tatggattag aaatagccat accaatgagc gctttctggt caattcgtgg
      961 attgaaaaca gcagcggtgt aaaagaaaag tcattcatca ttacaccgcc actgtttgtt
     1021 agtgaaccca aaagcgaaaa taccttgcgt attatttaca ccggtccacc gctggcggca
     1081 gatcgtgagt ctctgttctg gatgaatgtt aagacgatcc cttcggtaga taaaaatgca
     1141 ttgaacggca ggaatgtttt gcaactggcg attttatcgc gcatgaaatt atttctccgt
     1201 ccaattcaat tacaagaatt acccgcagaa gcgccggaca cactcaagtt ttcgcgatcc
     1261 ggtaactata tcaatgttca taatccatca cctttttatg tcaccctcgt taacctgcaa
     1321 gtgggcagcc aaaagttggg gaatgctatg gcggcaccca gagttagttc acaaattccc
     1381 ttaccctcag gagtgcaggg aaagctgaaa tttcagaccg ttaatgatta tggttcagta
     1441 accccggtca gagaagtgaa cttaaactaa ccgaatcatc tgacaatatc agagctaatt
     1501 atgaaaatac ccactactac ggatattccg cagaggtata cctggtgtct ggccggaatt
     1561 tgttattcat ctcttgccat tttaccctcc tttttaagct atgcggaaag ttatttcaac
     1621 ccggcatttt tattagagaa tggcacatcc gttgctgatt tatcgcgctt tgagagaggt
     1681 aatcatcaac ctgcgcgcgt gtatcgggtg gatctctggc gtaatgatga gttcattggt
     1741 tcacaggata tcgtatttga atcgacaaca gaaaatacag gtgataaatc aggtgggtta
     1801 atgccctgtt ttaaccaggt actccttgaa cgaattggcc ttaatagcag tgcatttccc
     1861 gagttagccc agcagcaaaa caataaatgc atcaatttac tgaaagctgt acctgatgcc
     1921 acaattaact ttgattttgc agcgatgcgc ctgaacatca ctattcctca gatagcgttg
     1981 ttgagtagcg ctcacggtta cattccgcct gaagagtggg atgaaggtat tcctgcttta
     2041 ctcctgaatt ataatttcac cggtaacaga ggtaatggta acgatagcta tttttttagt
     2101 gagttcagcg ggattaatat tggcccgtgg cgtttacgca acaatggttc ctggaactat
     2161 tttcgcggaa atggatatca ttcagaacag tggaataata ttggcacctg ggtacagcgt
     2221 gccattattc cgctgaaaag tgaactggta atgggagacg gcaatacagg aagtgatatt
     2281 ttcgatggtg ttggatttcg tggtgtacgg ctttattctt ccgataatat gtatcctgat
     2341 agccagcaag ggtttgcccc aacggtacgt gggattgccc gtacggcggc ccagctaacg
     2401 attcggcaaa atggttttat tatctatcaa agctatgttt cccccggcgc ttttgaaatt
     2461 acagatttgc acccgacatc ttcaaatggc gatctggacg tcaccatcga cgagcgcgat
     2521 ggtaatcagc agaattacac tatcccgtat tcaacagtgc caattttaca acgcgaaggg
     2581 cgtttcaaat ttgacctgac ggcgggcgat tttcgtagcg gtaatagtca gcaatcatcg
     2641 cctttctttt ttcagggcac ggcactcggc ggtttaccac aggaatttac tgcctacggc
     2701 gggacgcaat tatctgccaa ttacaccgcc tttttattag ggctggggcg caacctcggg
     2761 aactgggggg cagtgtcgct ggatgtgacc catgcgcgca gtcagttagc cgacgacagt
     2821 cgtcatgagg gggattccat tcgcttcctc tatgcgaaat cgatgaacac cttcggcacc
     2881 aattttcagt taatgggtta ccgctattcg acacaaggtt tttataccct tgatgatgtt
     2941 gcgtatcgtc gaatggaggg gtacgaatat gattacgatt acgacggtga gcatcgcgat
     3001 gaaccgataa tcgtgaatta ccacaattta cgctttagcc gtaaagaccg tttgcagtta
     3061 aatatttcac aatcacttaa tgactttggc tcgctttata tctctggtac ccatcaaaaa
     3121 tactggaata cttcggattc agatacctgg tatcaggtgg ggtataccag cagctgggtt
     3181 ggcatcagtt attcactctc attttcgtgg aatgaatctg tagggatccc cgataacgaa
     3241 cgtattgtcg gacttaatgt ttcagtgcct ttcaatgttc tgaccaaacg tcgctacacc
     3301 cgggaaaatg cgctcgaccg cgcttatgcc tcctttaacg ccaaccgtaa cagcaacggg
     3361 caaaatagct ggctggcagg tgtaggtggg accttactgg aaggccacaa cctgagttat
     3421 cacgtaagcc agggtgatac ctcgaataat gggtatacgg gcagcgccac ggcaaactgg
     3481 caggccgctt acgctacgct gggggtcggg tataactacg accgcgatca acatgacgtt
     3541 aactggcagc tgtctggcgg tgtggtcggg catgaaaatg gcataacgct gagccagcct
     3601 tttggggata ccaatgtttt gattaaagcg cctggcgcag gcggtgtacg cattgaaaat
     3661 caaactggca ttttaaccga ctggcgcggc tatgcggtga tgccgtatgc cacggtttat
     3721 cggtataacc gtatcgcgct tgataccaat acgatgggga attccatcga tgttgaaaaa
     3781 aatattagca gcgttgtgcc gacgcaaggc gcgttggttc gtgccaattt tgatacccgc
     3841 ataggcgtgc gggcgctcat taccgttacc cagggcggaa aaccggtgcc gtttggatca
     3901 ctggtacggg aaaacagtat cggaataacc agtatgtggg tgatgacggg caagtttatt
     3961 taagtggtgc gccattgtct ggtgaattac tggttcagtg gggagacggc gcgaactcac
     4021 gctgcattgc gcactatgta ttgccgaagc aaagcttaca gcaagccgtc actgttattt
     4081 cggcagtttg cacacatcct ggctcataaa ggaaattatc aatgaagata atctgcagat
     4141 tattattggc gatggcatgt ttgtgtctgg aaaacatatc ctgggctact gtttgtgcaa
     4201 atagtactgg cgtagcagaa gatgaacact atgatctctc aaatatcttt aatagcacca
     4261 ataaccagcc agggcagatt gttgttttac cggaaaaatc cggctgggta ggtgtctcag
     4321 caatttgtcc acccggtacg ctggtgaatt at
//
LOCUS       DAPHJE010000217         4349 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPHJE010000217 DAPHJE010000000
VERSION     DAPHJE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.219"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1101
                     /locus_tag="RH222_004366"
     CDS             <1..1101
                     /locus_tag="RH222_004366"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV3799281.1"
                     /translation="VNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKR
                     MEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENT
                     IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVW
                     DFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFS
                     RPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIP
                     AVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFA
                     ELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1257..2792
                     /gene="gadC"
                     /locus_tag="RH222_004367"
     CDS             1257..2792
                     /gene="gadC"
                     /locus_tag="RH222_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV3799282.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2923..4242
                     /locus_tag="RH222_004368"
     CDS             2923..4242
                     /locus_tag="RH222_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV3799283.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT         1025 a         1114 c         1105 g         1105 t
ORIGIN      
        1 gtaaatatgg ttgccgatct gtggcatgcg cctgcgccga aaaatggtca ggccgttggc
       61 accaacacca ttggttcttc cgaggcctgt atgctcggcg ggatggcgat gaaatggcgt
      121 tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata aaccaaacct ggtgtgcggt
      181 ccggtacaaa tctgctggca taaattcgcc cgctactggg atgtggagct gcgtgagatc
      241 cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca tgattgaagc ctgtgacgaa
      301 aacaccatcg gcgtggtgcc gactttcggc gtgacctaca ctggtaacta tgagttccca
      361 caaccgctgc acgatgcgct ggataaattc caggccgata ccggtatcga catcgacatg
      421 cacatcgacg ctgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
      481 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      541 ccgctgggct gcggctgggt tatctggcgt gacgaagaag cgctgccgca ggaactggtg
      601 ttcaacgttg actacctggg tggtcaaatt ggtactttcg ccatcaactt ctcccgcccg
      661 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctacacc
      721 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaactg
      781 gggccgtatg agttcatctg taccggtcgc ccggacgaag gcattccggc ggtttgcttc
      841 aaactcaaag atggtgaaga tccgggatac accctgtatg acctctctga acgtctgcgt
      901 ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga catcgtggtg
      961 atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt gctggaagac
     1021 tacaaagcct ccctgaaata tctcagcgat cacccgaaac tgcagggtat tgcccagcag
     1081 aacagcttta aacatacctg ataacgttta acggtaacgg tgtcccgaaa cgaacccgtt
     1141 tcgggacaat ttccaaagtc tgttcactgg cattagcaac ggaaaatatt gttctgaata
     1201 cgcttcagaa caaaacaggt gcggttccga caggaatacc gttttagggg gataatatgg
     1261 ctacatcagt acagacaggt aaagctaagc agctcacatt actcggattc tttgccataa
     1321 cggcatcgat ggtaatggct gtttatgaat accctacctt cgcaacatcg ggcttttcat
     1381 tagtcttctt cctgctatta ggcgggattt tatggtttat tcccgtggga ctttgtgctg
     1441 cggaaatggc caccgtcgac ggctgggaag aaggtggtgt cttcgcctgg gtatcaaata
     1501 ctctggggcc gagatgggga tttgcagcga tctcatttgg ctatctgcaa atcgccattg
     1561 gttttattcc gatgctctat ttcgtgttag gggcactctc ctacatcctg aaatggccag
     1621 cgctgaatga agaccccatt accaaaacta ttgcagcact catcattctt tgggcgctgg
     1681 cattaacgca gtttggtggc acgaaataca cggcgcgaat tgctaaagtt ggcttcttcg
     1741 ccggtatcct gttacctgca tttattttga tcgcattagc ggctatttat ctgcactccg
     1801 gtgcccccgt tgctatcgaa atggattcga agaccttctt ccctgacttc tctaaagtgg
     1861 gcacactggt tgtatttgtt gccttcattt tgagttatat gggcgtagaa gcttccgcaa
     1921 ctcacgtcaa tgaaatgagc aaccccgggc gcgactatcc actggctatg ttactgctga
     1981 tggtggcggc aatctgctta agctctgttg gcggtttatc tattgcgatg gtcattccgg
     2041 gtaatgaaat caacctctcc gcaggggtaa tgcaaacctt taccgttctg atgtcccatg
     2101 tggcaccgga aattgagtgg acggttcgcg tgatctccgc actgctgttg ctgggtgttc
     2161 tggcggaaat cgcctcctgg attgttggtc cttctcgcgg gatgtatgtc acagcgcaga
     2221 aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa tggcgtaccg gtaacgctgg
     2281 tcatttcgca gctggtgatt acttctatcg cgttgatcat cctcaccaat accggtggcg
     2341 gtaacaacat gtccttcctg atcgcactgg cgctgacggt ggtgatttat ctgtgtgctt
     2401 atttcatgct gtttattggc tacattgtgt tggttcttaa acatcctgac ttaaaacgca
     2461 catttaatat ccctggtggt aaaggggtga aattggtcgt ggcaattgtc ggtctgctga
     2521 cttcaattat ggcgtttatt gtttccttcc tgccgccgga taacatccag ggtgattcta
     2581 ccgatatgta tgttgaatta ctggttgtta gtttcctggt ggtacttgcc ctgcccttta
     2641 ttctctatgc tgttcatgat cgtaaaggca aagcgaatac cggcgtcact ctggagccaa
     2701 tcaacagtca gaacgcacca aaaggtcact tcttcctgca cccgcgtgca cgttcaccac
     2761 actatattgt gatgaatgac aagaaacact aaccatgtct gaatggttat aagacaaggg
     2821 agcgataagt catcgctccc tttttcgtgc ttgctgtcgt attgacttaa ccgggataag
     2881 tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat ctgctcccga
     2941 aacaagaaat taacgattag aagaccagcg atactagttg cactggcact tttactgtgt
     3001 agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc aaagccacca
     3061 gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac ggtttctcgg
     3121 ctcgactggc caccggtttc ctcggttaac attagtaacc ccaccagccg ggcccgtgta
     3181 caacaacagg cgatgatcga caaactggat catctgcaac gtctcggcat aaacacggtc
     3241 tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc
     3301 gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca atttatgctc
     3361 gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg
     3421 gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca acaaccggcg
     3481 agcgtctatg tgcaacaccg cgactggatc agaacgtctg gcgatcgctt tgtcctcgac
     3541 ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgcagaagt ggtttcccgc
     3601 tatcccgtag atggcgtgca gtttgacgac tatttctata cggagtcacc gggttcacgg
     3661 ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat ttgcgtcaaa agcagactgg
     3721 cggcgcaaca atactcagca gttaattgca aaggtatcgc acaccattaa aagcattaag
     3781 ccgggagtcg aatttggtgt tagcccggca ggcgtgtggc gtaaccgatc acacgatccg
     3841 ctcggttccg atacccgagg cgcggcagcc tatgacgaat cctacgctga cacccgtcga
     3901 tgggtggaac aaggattgct ggattacatt gctccccaaa tttactggcc gttctcacgg
     3961 agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa accgaccagg
     4021 acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccctcaaa gatagagcca
     4081 gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc agctcgatct taacgatgcg
     4141 gtaccagaaa ttagcggcac catcttgttc cgtgaggact atctgaataa accgcagact
     4201 caacaagcgg tcagctatct gcaaagtcgt tggggcagtt aaactcccat tcttactttg
     4261 taacaagccg gtgctttacc caccggcttg ttaccctctt gaactatccc actactaatc
     4321 atgcttacta aagtcaaatt aaccacgca
//
LOCUS       DAPHJE010000218         4241 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPHJE010000218 DAPHJE010000000
VERSION     DAPHJE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.220"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..579
                     /locus_tag="RH222_004369"
     CDS             7..579
                     /locus_tag="RH222_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799284.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTVIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            627..1358
                     /locus_tag="RH222_004370"
     CDS             627..1358
                     /locus_tag="RH222_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3799285.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1383..3905
                     /locus_tag="RH222_004371"
     CDS             1383..3905
                     /locus_tag="RH222_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3799286.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3916..>4241
                     /locus_tag="RH222_004372"
     CDS             3916..>4241
                     /locus_tag="RH222_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799287.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDL"
BASE COUNT         1158 a          914 c         1024 g         1145 t
ORIGIN      
        1 gtttttatga agcgtaatat tataggcggt gcattcactc tggcatctct aatgctggcc
       61 gggcatgcac tggcagaaga tggtgttgtt cacttcgtcg gtgaaattgt cgacactact
      121 tgtgaagtta cctccgatac agccgatcaa attgtcccac tgggtaaagt cagtaaaaat
      181 gcattttcag gtgtaggtag tctggcgtcg ccacagaagt tcagtattaa acttgaaaat
      241 tgcccggcaa cgtacactca agcagccgtt cgttttgatg gtacagaagc gcctggcggc
      301 gacggcgacc tgaaagtggg tacgccgctt acagcaggca accctggtga ttttaccggt
      361 acaggacaag cgattgcggc aaccggcgtt ggtattcgta tttttaacca gtccgataat
      421 tcgcaggtta aactttataa cgactctgct tataccgtta tcgatgctga aggcaaggct
      481 gaaatgaagt ttattgcacg ctatgtggca accaatgcga ccgtaacggc tggtacggcg
      541 aacgctgatt cacaatttac tgtcgaatat aagaaataat ttatcaaggg cctttttatt
      601 cagttggctt tggccaggga gtcgttatgt ccagaaaaag ttgtttaatc aggctcttta
      661 aaacaacagt ttcatgtaag ttatttaccg cgattatact cagcgttttt ttaggccaac
      721 cagcattaac ttatgcaggc gttgtcattg gtggtacgcg ggtggtttac ttaagtaata
      781 atgctgataa atctatttct gtatttagca aagaagaaaa aataccttat ctcatccagg
      841 cttgggtaga tccatttaat aaagaggata aaagcaaagc gccgtttacc gttattccgc
      901 cagtatcacg attagaacct tcgcaagaaa aaatattacg cattattcac accaaaggtg
      961 tgtcattgcc tgatgatcgc gagtcagtct tctggctgaa tattaaaaat attccgccat
     1021 cagcgagcaa caaagcgacg aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt
     1081 tctggcgacc agcaaatatc cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc
     1141 gccgggaagg gcgaaatctg atagcagaga atcccaaccc aattcatatc agtgtgatgg
     1201 atgtcatcgt tgatggccat gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca
     1261 cgttaccttt acctgctaat tctgcgggag gccaaatgac ctggcgattt attaatgatt
     1321 acggcgcagt cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta
     1381 ttatgatgac gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc
     1441 aaaacctgat ggccgaggtc agtgtattca atccggcgct tctggaaatc gaccatcaat
     1501 ccggagtcga tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg
     1561 ttgatatttt tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata
     1621 atccagatgc tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg
     1681 gcataaaagt tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc
     1741 ctgctccacg tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata
     1801 tatccattcc ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt
     1861 gggatgaggg gattaacgcg ctcaccatca actacgattt ttctggttca catacactac
     1921 gttcagatta tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga
     1981 atattggacc gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg
     2041 aatacaactc cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta
     2101 tgattggtga tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc
     2161 gtttgtatac tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc
     2221 gtgggattgc aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc
     2281 agtctgccgt tccacaaggt gcttttgaga tcaccgatct caacaccgcc agtacaggtg
     2341 gcgatttgga cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt
     2401 atgcttcatt ggcgattctt aaacgtgaag gtcagacaga tgttgatgtc agcgtgggtg
     2461 aattgcgcga tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt
     2521 tttcccacgg gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag
     2581 ctctgggtgt cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg
     2641 ctcgtgcgaa ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt
     2701 caaaacgatt tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg
     2761 agggctacta taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg
     2821 aaacaggtaa ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt
     2881 atggcaattt atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac
     2941 gattaatgca atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga
     3001 gttattccaa tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg
     3061 agcagatcta catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg
     3121 ccacctattc tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg
     3181 gtacggcgct ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg
     3241 atgatgaggt tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg
     3301 gcagctataa ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc
     3361 tggcacacag cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta
     3421 aagcacctgg ggccgccggg ttagaaatag ataatatgcg cggtgctgcg acggactggc
     3481 gcggctatac cgtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg
     3541 ataactattt ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca
     3601 cgcgtggtgc agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca
     3661 gagtgttaaa tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg
     3721 caagtttggc agactcagga attgtcggtg accgtggcga actttatctt tctggtctac
     3781 cagaaaaagg acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca
     3841 attattcact ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc
     3901 attaaaagga tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc
     3961 tcgttaagta gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta
     4021 tttcacagta cgcttgattt ctcgggatat cttattaccg cgaacgaaaa taaggttgga
     4081 acgacattta acaccactgt aactaatgga tcctcatatc ctggacgctg tcattgtgat
     4141 actggtaacg taggagaatt cccctatatc tattatacgt caaaaataaa ccaggcatta
     4201 acttatgctg gggttcactc taatattaat tattatgatc t
//
LOCUS       DAPHJE010000219         4190 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPHJE010000219 DAPHJE010000000
VERSION     DAPHJE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.221"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(243..581)
                     /gene="osmE"
                     /locus_tag="RH222_004373"
     CDS             complement(243..581)
                     /gene="osmE"
                     /locus_tag="RH222_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV3799288.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            783..1610
                     /gene="nadE"
                     /locus_tag="RH222_004374"
     CDS             783..1610
                     /gene="nadE"
                     /locus_tag="RH222_004374"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV3799289.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            1840..2727
                     /gene="cho"
                     /locus_tag="RH222_004375"
     CDS             1840..2727
                     /gene="cho"
                     /locus_tag="RH222_004375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV3799290.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2687..3262)
                     /gene="ves"
                     /locus_tag="RH222_004376"
     CDS             complement(2687..3262)
                     /gene="ves"
                     /locus_tag="RH222_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV3799291.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3465..3950)
                     /gene="spy"
                     /locus_tag="RH222_004377"
     CDS             complement(3465..3950)
                     /gene="spy"
                     /locus_tag="RH222_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV3799292.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1037 a         1011 c         1031 g         1111 t
ORIGIN      
        1 gcttccatgc tctgggtaac ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt
       61 atacggtata gacctgagca atagtatttc tttgaccttg taaattgtct ctccttgacc
      121 tgcggttatg accctcttca catttcgggc aaataattcg cgaagataat taagaactca
      181 ggccaaaaaa aaccggcgca atggccggtt tccgttgtta ctcaagcttt cagacgaagt
      241 gattacttcg cagcctgtgg atcagtgtcg tattcagcac aggtctgata accggagttg
      301 atgacatgtc cggtatcatc taacgcgaca aagtaggttt ctgctttacc atcacgttga
      361 cccagaatgt aggtctggca agtaccacga gcatggatca tgctcacttc agacgaaggt
      421 ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca
      481 ggctgtacaa actggtcttt ggtacgatca taagccgtac aacccgccag catggttaat
      541 accgccgctg cactcagaat tcctgccata ttcttgttca tattccgtcc tcttgtttat
      601 cagcgtgtta gataagcctg gaatacattg ggcgcttttt caagcccgtg aacgaaacgg
      661 ctccgctttc agaggattcc tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg
      721 tcgtttcagt agcaacgggt tagctttaag gaagttttgt cttttctgtc tggaggggtt
      781 caatgacatt gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag attaatgccg
      841 aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta cctgcgaact tatccgttca
      901 ttaaatcact ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc ggtaagctgt
      961 gccagatggc gattaatgag ctgcgccagg aaaccggcaa cgaatcactg caatttattg
     1021 ccgtacgcct gccctatggt gttcaggccg acgaacaaga ttgccaggat gccattgcct
     1081 ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg cgcggtattg gctagcgagc
     1141 aggcattgcg ggaagcaggc attgaactga gcgattttgt ccgtggcaat gaaaaagcgc
     1201 gtgagcggat gaaagcacaa tatagcattg cgggtatgac cagcggtgtc gtggtgggca
     1261 ccgatcatgc agcagaagcc attaccggat tcttcactaa atatggtgac ggcggtacgg
     1321 atattaatcc gctgtatcgt ctcaacaaac gccagggtaa acagttgctg acggcattag
     1381 gttgcccgga acacctttat aagaaagccc caacggccga tctggaagat gatcgccctt
     1441 ctctgccaga tgaagtggca ctcggcgtga cctatgacaa tatcgacgac tatctggaag
     1501 ggaaaaacgt acctgaacag gtcgccagaa caatagagaa ctggtatctg aaaaccgaac
     1561 ataaacgccg tccgccaatt accgttttcg atgatttctg gaaaaagtaa taatttgcac
     1621 ataatggttt gtgcgaaaaa gagtaatttg ttcacgccgg atgcggcgtg aacgccttat
     1681 tcgacctaca aaactatgca aattcaatat attgcaggag tcgaataggc ctgacaggcg
     1741 tagcacgtca gacggtgtaa cctttgtcat cgacccgcct cttttttaat cgcttcccgc
     1801 ctgttacact ggatagataa ccagcattcg gagtcaacag tggtacggcg tttaacttct
     1861 ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc ccgaacattt acgttcattc
     1921 cttaatgact tacccacccg accaggggtg tatctgtttc atggcgaaag tgacaccatg
     1981 ccgctctata tcggcaaaag cattaacatc cgcagccgcg tcctttctca tttacgtacc
     2041 ccggatgaag ccgccatgct acggcaatcc cgacgcatca gctggatctg caccgcaggc
     2101 gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc gctgtttaat
     2161 aaacggttgc gccgcaatcg ccagctctgt gccctgcaat taaatgaaaa gcgcgtcgat
     2221 gtggtgtatg ccaaagaggt ggatttttca cgagccccca acctgtttgg cctgtttgcc
     2281 aataggcgcg cagctttgca agcattgcag agcatcgctg atgaacaaaa actttgttat
     2341 ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa
     2401 cgttgcgccg gagcatgctg cggtaaagag agccatgagg aacatgcgct acgcttgcgc
     2461 cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc aaggtgcggt ggcactgaaa
     2521 gaacagcacc cggaaatgac tcaatatcat attattcaaa actggctgtg gctgggggcg
     2581 gttaattcgc tggaagaagc gacaacgtta attcggacac ccgccgggtt tgatcacgac
     2641 ggttataaaa ttctttgtaa gccgctgctt tccggtaact atgaaattac tgaacttgat
     2701 ccggcgaatg accagcgagc cagttgattt cactgaacaa cagtttacct tgcggttgta
     2761 aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta gtgagcaatt
     2821 tatcgcccag ttgccacgcc ccgttaatca caaacaccac cccgccgcgt gagccaaagg
     2881 tagtaaacgt acgttcggca attctgactt tcgccttaca gacatccagg cgtgtcatga
     2941 tgttgaaatc catcgacatc tgccctgccg tcagtttcgc ttttaccacc tggtccgctg
     3001 caaaggcaaa aggctgtaac ggttttaagg tatggttaaa gcggtctgcg ctttcaagga
     3061 gcatctcgcc gccttccagc aacgtcacta tcctttccat gccgggaaac aaagaaaact
     3121 ccccattcgc cgcgatggat gcaatgctgg cacgccagta aaaatcacgt tttgccggtg
     3181 ggaaagtgca aatttcgcgc gtttcaccgg cagcatttcg ccacagattc accgacattt
     3241 tacgcatatc aaagtattcc atactcgctc ccggcccgtt cttttcattg ttatcgttca
     3301 ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg ttagtggtga tcaggctttt
     3361 tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa
     3421 ggtagtggac aagaccggcg gtcttaagtt ttttggctga aagattattc agcagttgca
     3481 ggcattttac cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat
     3541 tgctttttct gttccggcgt caggatgttg taaattttgt tctgggtttc catgtgcgcc
     3601 agcatgttag ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta
     3661 tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc gttcttccag cggcggacgt
     3721 ttcatctggt cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc
     3781 aggttcaggt ctttgaacat catgtcctga tgcggaccga acttgccttt gtggtgcatc
     3841 atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc
     3901 gcgccaagag ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc
     3961 cttcctttca gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac
     4021 tttactgact ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac
     4081 acttttgcgc caattatgga gaaaaaaaga aaatttgatg gagagtgatg agagaatatt
     4141 acaacacgat gattttgcag agattatgaa gaactatacc ggatgactgg
//
LOCUS       DAPHJE010000220         4183 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPHJE010000220 DAPHJE010000000
VERSION     DAPHJE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.222"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..3165)
                     /gene="ag43"
                     /locus_tag="RH222_004378"
     CDS             complement(46..3165)
                     /gene="ag43"
                     /locus_tag="RH222_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3799293.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVLAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYTSMLTQAMDYDRILAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASSD
                     NNNFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGS
                     TQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHRVSELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            complement(3537..4130)
                     /locus_tag="RH222_004379"
     CDS             complement(3537..4130)
                     /locus_tag="RH222_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3799294.1"
                     /translation="MGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWR
                     HILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPV
                     VAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAVDRI
                     FDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          824 a         1254 c         1141 g          964 t
ORIGIN      
        1 gccccggtca tgatgaccgg ggcctgaagg agagtgacct gattatcaaa aagtcacatt
       61 cagcgtggcc tgaccgttat aaccttcagc gctgctgccg ctgacgctgt gggcataacc
      121 ggcctgaacg cccagggtga tattttcccg gacacgggct tccagtccgg cctgcaggtc
      181 cagtgacgtg ccattccggg acggtgagaa cgtcatgtta ctgccggcgg cggctgtacc
      241 catgctcatg tcaccccggg agctgaaagt gcggataaca gaaggctgta cccaccagtt
      301 caccggcagt tcactcacac ggtgttttgt actgtcgcgc agggtgtcac gggatgaggt
      361 gccttcgcca aaggtcatat cgttgtggct gcccagacgg aagccggcac gcacatgttg
      421 tgtactgcca tgcccgaact tcacataacc ggcgttatcc tggccgtcat ccagggagag
      481 cccctgccag gtgtactgca gttgtggctc cagcatcagg ttgtcagtga tactgaaggg
      541 cagaccggtt tccagtgagc ccagccagcc ccagccccgg gcgcggaagt tgttattgtc
      601 cgatgacgct ttcatgctgt ggcgggttcc ctgtgccaca atgtcagccc acaggccgga
      661 ggacgtgtgt accagattca ggtatccgcc cagactgccg gcatcatccc ggaccgtgcc
      721 ggcgcgggaa ccgtcatcat ccttaacatc aacggaagaa tggcctgcgg caccatacac
      781 ccctgtcgtc agagacatac cggcaacctc tgttctcagc aggtcaccct ccagacggac
      841 gaagccatag ctgccgctgc tttccggcgt ggctccacgg gcaataccgc cgttgttatc
      901 gtgaccgaga tgaccgccct gaatgctgag acggacgctg ttattttcac cgtttacacc
      961 ggtctgatgg ctgcgggagc ctgccagaat ccggtcatag tccattgcct gtgtcagcat
     1021 ggatgtatac agggggactt cagcacgata agcattttca ctgcgcagat accagctctc
     1081 atcactgtcc cggttgaggg agtagttaaa ggcaccggcc tgcagcttat tcccctggat
     1141 aaaggcccct tcctccgtgg tggcaccgtt aatggcttcc accacctgaa tacctttacc
     1201 gctggtcgcc agccccgacg cactgttgcc ggcgttcacc aggttcagga tggtttttcc
     1261 ggttgccctg ccaccgtcaa tgaccagtct gtcagcattg ttctgtgcca tatccgggcg
     1321 tacacgcagg ctgatggtgc cattctgtcc gttcaggttt ttcactttca gggttgccgg
     1381 tacgaacttc cctgtgcggg tggaggtgaa atgaatctgt ccggcatggc tgaggtcatc
     1441 caccaccgac tgcaccgtgg cgttatcggg aatattccag gtggcaccag aggcgagagt
     1501 gacattcgtg gggtcaatgg caccgttcag cacagtgctg ccggtcagct tcagggctgt
     1561 accgcgctga gcaatgacat ccgtggtgac ggtactgtcg ttcagcgtca gcgtgccttc
     1621 attcaggttg acggctttct gggtgagtgt ggtgttgctg acagtgagtg tgccacttcc
     1681 tgatttttcc acgctgccgt taccggtgag agagcctccc tgcaggagtg catcgccttc
     1741 acggatggtc agggtatcgt tgttcaccgt cttcccggaa agggtaagta tggcgccgtt
     1801 attcagcgtg gtggtgcccg ccagtgtgcc gcccctggcg gtgaacagtc cgccatttac
     1861 cgtggtatcc gtggccgtat caccggcgtt cagcgtgaat gaactgcctt tttccagcat
     1921 cagggcatcc gcctgaccgc ctccgatact gaatgccttt ccgtcgctcc gggtaccact
     1981 gacagcggca ccggaatcgg ccagcagtac accgccattt cccatggata cggtggtggc
     2041 ggtgccaccg ttgcggacat ccagcgttcc gccatcatcc acgcgggtat tagtggctgt
     2101 gtgtccggtc agcacatcca ggcgtccgcc attttccagt acgacattat cagctttacc
     2161 ctccacaaca gagaatgctc ccaggcggtt tatgccggta acggttgcag ccgtactggt
     2221 aaccagtgcg ccgccctgct tcagggtgac attcgtggct gtaccaccgg cgtccacctg
     2281 cagtctgccc ttctgattaa cggtggtatt cccggccaca cccccgtttt tgacaatctg
     2341 ccagccgtca ctgtttacaa cagtgccaga cgccgtaccg ccgttgtgca catactggta
     2401 ccccccattc agcgtggtat ccagtgcgtg accatgtaca gtctggtcgc cgccggcata
     2461 aaccacagtg gtatttgccg ttccttcagc agccacaatc tgacgaccat ttttattgat
     2521 ggtggtgcgt acggcatctc cgtagacggt ctgcccggta tcaccatttt ccgcatccgg
     2581 tcccccttcc gcgccggtat tcacaaccgt gtcggttgcc actgcgccgg atttgatggc
     2641 ctgccagccc ttctcattaa tgacggtacc cgttgcaatc ccgccttcat gtacccactg
     2701 ctcaccgccg ttcagagtgg tgttcactgc ctgcccctga aggctctgtc cgcctccggc
     2761 actgataacc gtgtctgaaa cgcttcctcc ggcattcact ctctgaagac caccaccggt
     2821 gacagtagtg ttgttggcga taccgccatt ttgtatccat tgtccgccgg tattggcctc
     2881 gttatccggc ccatactcca gaccggtact gatggtcatt ccgttggccg taccgaggac
     2941 aatctggttg tcatgatttg tcagtgttcc gtcgttcacg gtttctcccg ccagtacaac
     3001 cttgtcagca gccagtgccg ggactgatgt gacagcagca agagacagcg caaccgccac
     3061 accagcgcgt tttccccgtg agcgggccag ttcggaggcc accaccaggg tgcccgtaat
     3121 gtgattccat accagcctgt agctggtgtt cagatgtcgt ttcatcagct tttccttaca
     3181 gagggtgaat aaaaaagccg gtacccacaa atgtggatac cggcaggtac aaaacagcat
     3241 gctcagataa agtggttttt cccggacata aatgcttccc ctctctcatc cgccagagaa
     3301 gtatcagtcc gttgtgtgca attccgttct gcatgatgaa gagattcagc tgccttttcg
     3361 cagaccatta accactataa acgatcgata gcaacgatcg atacctgttt tatcgatcgt
     3421 tttattctat taacgacaag gcggtcacgc aagagaaaca ggaggagtat gaggggtttg
     3481 tgtcatgttt tcagttggtt aacgtaatgt tatcaggcat ttccctggca gactgttcag
     3541 aagaaaatcc agttccatac agcgcgggca acagagacca ccgtgtcacg gacggcacgc
     3601 agaaccgtac gggcaacaga ggcaacacag acgctctccg ccgtgtcaaa tatccggtcc
     3661 accgcaccgg taaactgttc acgggcctga gcgcggacag attccgtgcg cagctcgtcc
     3721 tgcaggcggg tcatcagggg actggcggca tggtcgggaa gtgctgtcat tagcgcactg
     3781 accagcgtat ccagttccca gccggtgcgg gccgatacgg caacaaccgg atgtacgggc
     3841 cggaacagac ggaataccgc ctccgttttc tcccgaatgt tctgtgcctg tgcgggagaa
     3901 ggctgaatgc cggccatatc ccattcatgg cagggctccg ttttgtcggc ctgcgtcacc
     3961 acaaacagca cctgctgatg tccccggtgc aggatgtgtc gccagaaata ctcatccaca
     4021 gacagggcgc ggtcatcggc tttaatcagc cacagcacca ggtccagttc gggcagaatg
     4081 tcacggtaca gggcttcata ctctgcatcc ctgtcccggc tctcgcccac cccgggcagg
     4141 tcagtgataa ccatgctgtg accgtggcca ctcagacgga agc
//
LOCUS       DAPHJE010000221         4167 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPHJE010000221 DAPHJE010000000
VERSION     DAPHJE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.223"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            246..854
                     /locus_tag="RH222_004380"
     CDS             246..854
                     /locus_tag="RH222_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV3799295.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            952..2343
                     /gene="selA"
                     /locus_tag="RH222_004381"
     CDS             952..2343
                     /gene="selA"
                     /locus_tag="RH222_004381"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV3799296.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2340..>4167
                     /gene="selB"
                     /locus_tag="RH222_004382"
     CDS             2340..>4167
                     /gene="selB"
                     /locus_tag="RH222_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV3799297.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT          937 a         1055 c         1222 g          953 t
ORIGIN      
        1 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
       61 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
      121 ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac aaaccatcaa
      181 cattttctgc cctttttccc atcaccaggc catactgata tttttcctcg cacaaaggac
      241 tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt tctattctgt
      301 tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac gcggacaacg
      361 gcgtggcgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa gagggcgaat
      421 gttggtttga ttcgccgatc atcgctgaat atattgaatt aatgaatgtc gctccggcga
      481 tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggca ctggcggatg
      541 gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccagcg gcgcagcagt
      601 ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag
      661 gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact atcgccattg
      721 cctgtgccgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc
      781 cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac
      841 cgccaaaggc ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca atccgtggtt
      901 atgttaaacg cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc
      961 gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc
     1021 ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg
     1081 ctcgacgaag cgcgagaagt gattcgtggc agccagacgc tgcctgcgtg gtgtgaaaac
     1141 tgggcgcaag aagtcgatgc ccggttgacg aaagaagcgc agagcgcgct gcgtccggtg
     1201 atcaacctga cgggaaccgt gctgcatacc aaccttgggc gagctttaca ggcggaagcc
     1261 gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta cgatctggac
     1321 gacgccggac gcggacatcg cgatcgggcg ctggcgcaac tgctgtgccg tattacgggg
     1381 gcggaagatg cctgtatcgt caataacaat gcggcggcgg tgttattgat gttggcggcc
     1441 actgccagcg gaaaagaggt ggtggtatct cgcggcgaac tggtggagat tggcggcgcg
     1501 tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt agggaccacc
     1561 aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc actgttgatg
     1621 aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga tgaagcggaa
     1681 ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg
     1741 ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg
     1801 gcgggcgtca gtctggtgag tttctccggc gacaagttgt taggcgggcc gcaggcagga
     1861 attattgttg gtaaaaaaga gatgatcgcc cgcctgcaaa gccacccgct gaagcgtgca
     1921 ttacgcgcgg ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct ttatttacac
     1981 cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc
     2041 attcaaatcc aggcacaacg tttacaggcc ccccttgccg cacattacgg cgcggagttt
     2101 gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc
     2161 ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca
     2221 ttagccgccc gctggcgtga attgccagtg ccggtgattg gtcgtattta tgacggacga
     2281 ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat gttgttgaaa
     2341 tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg caggcgatta
     2401 ctggcgtaaa tgctgaccgt ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg
     2461 gctatgccta ctggccgcag ccggatggtc gcgtgcctgg ttttatcgac gttcccggtc
     2521 atgaaaaatt tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg
     2581 tggtggcgtg cgatgacggt gtgatggcac agacccgtga gcatctggcg attttgcagc
     2641 tgaccggtaa cccgatgctg acagtggtgc tgaccaaagc cgatcgcgtg gacgaagcgc
     2701 gtgttgatga ggttgaacgt caggtaaagg aggtgctgcg ggaatacggt tttgctgagg
     2761 caaaactgtt tatcaccgca gcaaccgaag gtcgaggaat tgatgccctg cgcgagcatc
     2821 tgcttcagtt accagaacgt gaacacgcca gccaacatag tttccgcctc gcgatcgacc
     2881 gcgcatttac cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta agcggggaag
     2941 tgaaggtagg cgattcacta tggctaactg gtgtaaataa accgatgcgt gtacgtgcgc
     3001 tgcatgcaca aaaccagcca acagaaaccg cccatgccgg gcagcgtatc gcgcttaaca
     3061 tcgcgggtga tgcggaaaaa gagcagatta accgtggcga ctggctgctt gccgatgcgc
     3121 cgccagagcc gttcacacgg gtgattgtcg agcttcaaac ccatacgccg ctgacccagt
     3181 ggcagccgct gcatattcac cacgccgcca gccacgttac gggacgcgtt tcgctgctgg
     3241 aagataacct tgccgagctg gtgttcgaca cgccgctgtg gctggcggat aacgatcgct
     3301 tagtgctgcg cgatatctcg gcccgcaaca cgctggcggg ggcgcgtgtg gtgatgctta
     3361 acccgccgcg tcgcggtaaa cgtaagccgg aatatctgca atggctggcg tctcttgcac
     3421 gggcgcagag tgatgccgat gcgttatctg ttcatctgga acgcggcgcg gttaaccttg
     3481 cggatttcgc ctgggcgcgc cagctcaacg gcgaagggat gcgcgaattg ctgcaacagc
     3541 ctggctatat tcaggctggt tatagcttgt tgaatgcgcc ggttgccgcc cgctggcagc
     3601 ggaaaattct cgacacatta gcgacttatc atgagcaaca tcgcgatgaa cctgggcctg
     3661 ggcgcgaacg tctgcgacgt atggcgttgc caatggaaga tgaagcgcta gtactgttgc
     3721 tgattgaaaa gatgcgcgaa agcggcgaca tcctcagtca tcacggttgg ctgcatctgc
     3781 cggatcacaa agcgggcttc agcgaagagc agcaggccat ctggcaaaaa gcagagccgc
     3841 tgtttggtga cgagccgtgg tgggtgcgtg atctggcaaa agagacgggt accgacgagc
     3901 aggcaatgcg cctgacgcta cgccaggcgg cgcagcaagg aataattacc gcgatcgtca
     3961 aagatcgtta ttaccgtaac gatcggattg tcgaatttgc caatatgatc cgcgatctcg
     4021 atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa
     4081 agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt cgtcgtggaa
     4141 atgatcattt attgcgcgac gcattat
//
LOCUS       DAPHJE010000222         4140 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPHJE010000222 DAPHJE010000000
VERSION     DAPHJE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.224"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="RH222_004383"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="RH222_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3799298.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="RH222_004384"
     tRNA            complement(249..324)
                     /locus_tag="RH222_004384"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="RH222_004385"
     tRNA            complement(331..405)
                     /locus_tag="RH222_004385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="RH222_004386"
     tRNA            complement(522..606)
                     /locus_tag="RH222_004386"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="RH222_004387"
     tRNA            complement(615..690)
                     /locus_tag="RH222_004387"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="RH222_004388"
                     /gene_synonym="panK"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="RH222_004388"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV3799299.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="RH222_004389"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="RH222_004389"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV3799300.1"
                     /translation="MKDYTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="RH222_004390"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="RH222_004390"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV3799301.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1073 a         1031 c          921 g         1115 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgcgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg cagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtactaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttacc gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 gattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgtgagcgcg ccagtttaat cctgacgaaa agtgctaatc atgcggtaga
     1981 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagccc cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccagctttt cccagcgcga cagataaggt
     2221 gccaatcctt cttgttcgaa gagttccaac gcagcacgta attcacgtat tagcatggcc
     2281 gccaacgtat tacgatcgag attgatcccc gcttcctgca gcgtgatcca cccctgatta
     2341 acgacactct cttcaacacg gcgcattgcc atgttgatcc cggctccaat gactatttgc
     2401 gccgcatcgc cagttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttgacacga actttatctg ctcccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgcccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa ctggtagcac ggctacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatggg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcaccgt tcgctaacag ggcaatcaat ttcagtggca cggtgtaatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatggtg tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagaa ctaacgcctg ttgacggtgc accgcagccc
     3181 cacccatttg catccctttt agctggcact gatcaataag ccaacctgct gccagtttta
     3241 ctgaaccatc cgcctggggg taatttggtg ctgttggaaa ttgtgacagt aatgcttcag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
//
LOCUS       DAPHJE010000223         4098 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPHJE010000223 DAPHJE010000000
VERSION     DAPHJE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.225"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..3945
                     /locus_tag="RH222_004391"
     CDS             <1..3945
                     /locus_tag="RH222_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3799302.1"
                     /translation="KAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLW
                     LARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEV
                     LPPPPPAYRVLTRVVDGFGRTLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRA
                     EAFRKQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDE
                     QPTAPLARYTYTAGGELRAVYDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDD
                     TGRVTEQVNPEGLDYRFEYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSIT
                     RSEYDEAGRLKAQTDAAGRRTEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTY
                     PDGLRSSREYDERGRLTAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYG
                     QLLAFTDCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYE
                     YSAAGDLTATVSPDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQ
                     STFRYDPVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVN
                     GEPAEQWQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGEMLWEH
                     ETGHAYSEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARS
                     FGGAGSTAGYEQATAYTLTGQLQSRHLNLPQLDRDYTWNDNGQLVRISGPQECREYRY
                     SGTGRLTGVHTTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVY
                     RYDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYD
                     PLGRRTGKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSF
                     TPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESE
                     AWLAQCGLTVEQMESQMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWG
                     NQLNEENPPHLYQPYRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLY
                     TYPLAPIRYTDPLGLERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMT
                     FDCSNRIDRRSQRGAGDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSS
                     LRDPYAPRQGWKPTMGCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
BASE COUNT          924 a         1056 c         1422 g          696 t
ORIGIN      
        1 aaagcgccct tcgatatccg cttacagata cgcgatgaag gcctgatact caacgacagc
       61 ggcggcagga gcattcactt tgagccgctg tttcccggcg agataagcta cagccgcagc
      121 gagtcactct ggctggcgcg gggcggagta gcggcgcagc acagttcgca gccgctaagc
      181 gcgctctggc aggtgctgcc ggaagatgtt cgcctgagtc cgcatgtgta cctggcgaca
      241 aacagccttc aggggccgtg gtggatactc agctggccgg agcgggtgcc gggggcggac
      301 gaggtgctgc cgcctccgcc gcccgcatac cgggtgctga cgagggtggt ggacggcttc
      361 gggcggacgc tggcctttca ccgggcagct aagggtgatg tggcgggcgc ggtgacgggg
      421 gtgacggacg gcgcgggccg ccgttttcac ctggcgctga ccacgcaggc gcagcgggcg
      481 gaagccttcc gtaaacagcg cgcctcttcc ttatcttccc cggccagtcc ccgttctgtt
      541 tcctcttctc aggttttccc cgacacgctg cccgccggta cagagtacgg tgccgataac
      601 ggtatccggc tggaggcggt atggctgaca cacgacccgg catacccgga tgaacagccg
      661 actgcgccgc tggcgcgcta cacgtacacg gccggtggtg aactgcgggc ggtgtatgac
      721 cgcagcggaa cgcaggtgcg cgggtttgct tatgatgcgg agcacgccgg gcggatggtg
      781 gcgcaccatt atgcggggag gccggagagc cgctaccggt atgatgatac cggccgggtg
      841 acggagcagg tcaacccgga ggggctggac taccgctttg agtacgggca ggaccgtgtg
      901 accatcacgg acagcctgaa ccggcgggag gtgctgtaca cggagggcga gggcggcctg
      961 aagcgggtgg tgaagaagga gcatgccgac gggagcatca cccgcagcga gtatgatgag
     1021 gcggggaggc tgaaggcgca gacggatgcg gcggggcggc ggacggagta ccggctgcat
     1081 atggcgtcgg gcgcggtgac agcggtgacg gggccggacg gcaggacggt gcggtatggc
     1141 tataacagcc agcggcaggt gacgtcagtg acgtacccgg acgggcttcg cagcagccgg
     1201 gagtatgatg agaggggaag gctgacggcg gagacctcgc gcagcggtga gacaacgcgg
     1261 tacagctatg atgacccggc gagtgagctg ccgacaggga tacaggacgc gacgggcagt
     1321 acaaaacaga tggcatggag ccgttacggt caactgctgg ccttcacgga ctgctcgggg
     1381 tacacgacgc ggtatgagta cgaccggtac ggtcaacaaa tcgccgttca ccgggaggaa
     1441 ggcatcagca cttacagcag ttataacccg cgtgggcaac tggtcagtca gaaggatgcg
     1501 caggggcgtg aaatccgtta tgagtacagc gccgcaggcg acctgacagc aacggtatca
     1561 ccggacggta aacgcagcac catcgaatat gataaacgcg ggcgtccggt gagtgtgacg
     1621 gaaggcggtc tgacgcgcag catggggtat gacgctgccg ggcgcatcac tgtgctgacc
     1681 aacgagaacg gcagccagtc cacgttccgg tatgacccgg tcgacaggct gacggagcag
     1741 cgcggctttg acggccggac gcaacgttac cagtatgacc tgaccggaaa actcacgcag
     1801 agtgaagatg aggggcttat caccctctgg cattacgatg cgtcggaccg tatcacccgg
     1861 cgcacggtga acggtgaacc ggcggagcag tggcagtatg acgaccacgg ctggctgaca
     1921 gaaatcagcc acctgagtga aggccaccgt gtggcggtcc actacggcta tgacgataaa
     1981 ggccgtctga cgggtgaacg gcagacggtg gagaacccgg agacagggga gatgctgtgg
     2041 gagcatgaga cggggcatgc gtacagcgaa caggggctgg cgacccgtca ggagccggac
     2101 ggtctgccgc cggtggagtg gctgacgtat ggcagcggtt atcttgcggg gatgaagctg
     2161 ggcggaacgc cgctggtcga gtacacgcgg gaccggctgc accgcgagac ggcccgtagc
     2221 ttcggcgggg cgggcagtac agcggggtac gaacaggcca cggcgtacac cctcacaggg
     2281 cagctacaga gcaggcacct gaaccttccg cagcttgacc gtgactatac gtggaacgat
     2341 aacggacaac tggtgcgcat cagcggcccg caggagtgcc gggagtaccg ttacagcggc
     2401 acgggaaggc tgacgggcgt ccacactacg gcagcgaacc tggatatcga tatcccgtat
     2461 gcgacggacc cggcaggaaa ccggctgccg gacccggaac tgcacccgga cagcaccctc
     2521 acggcgtggc cggataaccg catcgcggaa gatgcgcact atgtctatcg ctacgatgaa
     2581 tacggcaggc tggcggagaa gacggaccgc atcccggaag gggttatccg gatgcacgac
     2641 gagcggaccc accactacca ttacgacagc cagcaccgtc tggtgttcta cacgcggata
     2701 cagcacggcg aaccacaggt ggagagccgc tacctctatg acccgctggg ccgccggacg
     2761 ggaaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa
     2821 ccggaggaga cctggtacgg gtgggacggt gacaggctga ccactgtaca gacccaacag
     2881 acaagaatcc agacggtata ccagccggga agcttcacgc cgctcctgag aatcgaaaca
     2941 gagaatggcg aacaggcgaa ggcgcggcac cggagcctgg cggaggtgtt gcaggaggac
     3001 acgggggtga cgcttccggc ggagctggcg gtgatgctgg ggaggctgga gcgggaactg
     3061 cgtgcgggtg cggtgagtgc ggagagtgag gcatggcttg cgcagtgcgg gctgacggtg
     3121 gagcagatgg aaagccagat ggaggcggaa tacatcccgg agcggaggct gcatctttac
     3181 cactgtgacc accggggact gccgcaggcg cttatcagcc cggaagggga aacggcgtgg
     3241 cgcggggagt atgacgagtg ggggaaccag cttaatgagg agaacccgcc tcacctgtac
     3301 cagccgtacc gtttgccggg gcagcagtat gatgaggagt cgggactgta ttacaaccgt
     3361 caccggtact acgatccgtt gcaggggcgg tatatcaccc aggatccgat agggttgaag
     3421 gggggaataa atctatacac gtatccactg gcaccaatac gttacactga tccactgggg
     3481 ttagaacgag ttataagtgt atatgggcct cccgctccag accgggcagg tgctgaaact
     3541 cctcttgttc ttactgatat gactggagga gtgactattt attatgatcc tgaaacaggt
     3601 gattcaatga cttttgattg tagcaataga attgatcgtc gttctcaacg tggtgctggt
     3661 gatccatata caggtgaagt cgttggttgt gagacaaatg gatcaggaat atcggcagca
     3721 tatggcacaa cgaagatcta tactacagat actagagcaa gatggctaca tggtggggga
     3781 tcatcgttgc gcgatcctta tgctcctcga caaggctgga aaccaactat gggatgtacc
     3841 cgtgcacaga atgaagatgt agatgagttg tgtaagaaag tgacaagttg gatgtattcg
     3901 catcctggag agagaattag atatgaacgt tttaagacac gttaatgcga ttaacatggg
     3961 agtgtgcatt ctaattttgg ttattggatg tgcaaatgct aaagttctta aacgatgtat
     4021 attacccgct cctgatggtg gcgtagaaaa actatcaaaa cccaacttac atggagtaat
     4081 taaaaaagtt gatttgaa
//
LOCUS       DAPHJE010000224         3956 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPHJE010000224 DAPHJE010000000
VERSION     DAPHJE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.226"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..1707
                     /gene="dpiB"
                     /locus_tag="RH222_004392"
     CDS             49..1707
                     /gene="dpiB"
                     /locus_tag="RH222_004392"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV3799303.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1676..2356
                     /gene="dpiA"
                     /locus_tag="RH222_004393"
                     /gene_synonym="citB"
     CDS             1676..2356
                     /gene="dpiA"
                     /locus_tag="RH222_004393"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV3799304.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2397..3782)
                     /gene="dcuC"
                     /locus_tag="RH222_004394"
     CDS             complement(2397..3782)
                     /gene="dcuC"
                     /locus_tag="RH222_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV3799305.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1080 a          939 c         1022 g          915 t
ORIGIN      
        1 tttaattaca tccgtcaaag aggctcggga caacccgcaa ggaaaacaat gttgcagctt
       61 aacgagaata aacagtttgc atttttccaa agactggcat ttccgctgcg tatctttttg
      121 ctgattctgg tgttctcaat atttgtcatt gcagccctgg cgcaatattt tacggccagt
      181 tttgaggact atttaacgct tcatgtacgc gacatggcaa tgaatcaggc gaaaattatt
      241 gcctccaatg acagtgtcat ctctgcggtg aaaacgcgtg actacaaacg gctggcgacc
      301 atcgctaaca aattacaaag agataccgat tttgattatg tggtgattgg ggaccggcac
      361 tcgatccgcc tttaccatcc taatccggag aaaattggtt atcctatgca gttcaccaaa
      421 cagggcgcgc tggagaaagg ggagagctac ttcattaccg ggaaagggtc aatggggatg
      481 gcgatgcgcg ccaaaacgcc aatctttgat gacgatggaa aagtcatcgg cgtggtgtcg
      541 attggctacc tggtgagtaa aatcgatagc tggcgggctg agtttttatt accgatggca
      601 ggcgtgtttg tcgtgctgtt agggattctg atgttgctgt cgtggttcct ggcagcgcat
      661 atccgtcggc aaatgatggg catggagcca aagcaaatcg cacgcgtggt ccgtcagcaa
      721 gaggcgctgt ttagttcggt ttatgaaggg ctgattgcgg tggatccgca tggttacatt
      781 accgccatca atcgtaacgc aagaaagatg ctggggctga gctctcccgg acggcaatgg
      841 ttgggtaaac ccattgctga agtggtcagg cccgccgatt tctttaccga acagattgat
      901 gaaaaacgtc aggatgtggt ggctaacttt aacggtctga gcgttattgc caatcgggaa
      961 gctattcgtt caggtgatga tttgctgggg gccattatca gctttcgtag taaagacgaa
     1021 atttccaccc tcaatgcgca actgacgcaa ataaaacaat acgtcgagag ccttcgtaca
     1081 ttgcgacacg agcatctcaa ttggatgtcg acgctcaatg gtctgttgca gatgaaagag
     1141 tatgatcgcg tgctggcgat ggtgcagggg gagtctcagg cccagcaaca gcttattgac
     1201 agcctgcgtg aggcgtttgc cgatcgccag gtggcggggc tgctttttgg taaagtgcag
     1261 cgcgcccggg aactggggct aaaaatgatc attgtccccg gtagccagct ttcgcaactg
     1321 ccgccaggac tggatagcac cgagtttgca gccattgtgg gcaatttact tgataacgcc
     1381 ttcgaagcca gcctgcgtag cgatgaagga aacaagatcg ttgaattatt cctcagcgat
     1441 gaaggcgatg atgtggtgat tgaagtcgcc gatcagggct gcggcgttcc agagtctcta
     1501 cgagacaaaa tatttgagca gggggtcagt acgcgtgctg acgagcccgg tgaacatggc
     1561 attgggttgt acttgattgc cagctacgta acgcgctgcg gtggtgttat cactctcgaa
     1621 gataatgatc cctgcggtac cttgttttca atctatattc cgaaagtgaa acctaatgac
     1681 agctccatta accctattga tcgttgagga cgaaacgccg ctggcagaga tgcatgcgga
     1741 atatattcgt cacattcccg gattcagtca gatattactg gcgggaaacc tggcgcaggc
     1801 ccgaatgatg atcgagcgtt ttaagccggg gctaatcttg ctcgataact atcttcctga
     1861 cggcagaggg attaatttat tgcatgaact ggtgcaggcg cattatcccg gcgacgtggt
     1921 gtttaccact gcagccagcg atatggaaac ggtgtctgaa gccgtacgtt gtggtgtatt
     1981 tgattatctc attaagccca ttgcctatga acggctgggg caaacgctaa cccgtttccg
     2041 ccagcgtaaa catatgctgg aaagtattga tagcgccagc cagaagcaaa ttgatgagat
     2101 gtttaatgcc tatgctcgcg gtgaacctaa agacgaactg ccgaccggca ttgatccctt
     2161 gacgctaaac gcggtgcgaa aactgtttaa agagcctggt gtgcaacata cggcagaaac
     2221 ggtggcgcag gcactgacca tcagtcgcac cactgccagg cgttatcttg aatattgcgc
     2281 cagccgccat ctgattattg ctgaaattgt tcacggcaaa gttggcagac cacaacgcat
     2341 ataccacagt gggtgacatg ataaagccgg aggaaacttg cctccggcat tactatttac
     2401 ttgcctgtga ccgctgctgc cgttcctggc accatcagct ctgtagcaac gataacaatc
     2461 actagcccaa caagcaccgg caccgaggtg cgttttacga cttcaaatgg tgagattttc
     2521 gccatcccgg caaccgcaac gactacgcca gaaacgggtg atagggtacg acccaggttt
     2581 gacgcctgca gcatcgggat agtcaaatac gccgggttaa tgccggaaga gtgcgccagt
     2641 ttcgggatca tctcaacaaa cgcataaaac ggcgcattgc ctgaaccggt cgtgactgcc
     2701 gccagcatgg tcagaatcac caataccagc atcaggatga tactcgccga accaaacgag
     2761 gtagcgatag aaatcagact ttgaataaag ccgatggtgc taagcccctg agcgaatacc
     2821 ccagcggcaa ccagcagcat caccacgtta gcaaacgcat ctgccatccc gcgataagcc
     2881 acttccagac cagagaaaac tttctgggta ttaaagctgc ggaggaactc cagaatggag
     2941 gcaatcagca tacaaatcac cagaatagtg atgatgtgta attgcggacc ccatttaccg
     3001 tcaaaaatca gtacaccgat gatcggcgtg aacggcaaaa tggcataaaa cgcaggagca
     3061 gtggtggtga tttcactgac atctaacatt tcatgagaga tgtgctcttt tttatccaga
     3121 taacgttgcc agaagaagtg ggcgatcgcc atgccgataa ttgcagcaat tgagataggc
     3181 agcgtcgttt tgaaggcgaa gtcaatcagc gacatttcgg aagcttgcgc cgccagcacc
     3241 acatcccctg aagtcggtgc gagaataatc gccgccgggg aggcacaaat ggcagcagct
     3301 gcgccacgac tgataccaac gtttaccatc accggaaata gggttgccat cagcaaaaca
     3361 cccagaccgg ttgcggaaga gacggccaga gacatcagac aggcgacaaa ataggcggca
     3421 atcatcagca ggtaagggga gttaatatac tgcaatggtt ttgacgccag cttgaccacc
     3481 atatcattcg cgccgatatg ggtcatgtaa gcggcaaatc cacacagcat cataatcatc
     3541 atgccgaggt cgccgccgcg gctcattagt aatattttaa cgtattcaac gatatccgtg
     3601 gcgctgtagc ctgttgaagc ctggctggac ggtaacactt tgtgccccat aatggcactg
     3661 ataatcagca ataacaggcc accgacaaat aacacaccag tggcggaata ccctttaatg
     3721 atgtagcgag ctacacccac aataaccaca accccaataa ggagctcaat gaatgtcagc
     3781 ataatttttc ctgtctccag gccccaaagt aaataataaa aaattcctaa agcttaagga
     3841 aaaaatatgc ccaataaatt ggcgatgaat gctgattaaa atcaagaaaa actgccatta
     3901 agacattgaa gttgctgttt ttatacatag ataacaaaac gcatatttag cggttg
//
LOCUS       DAPHJE010000225         3912 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPHJE010000225 DAPHJE010000000
VERSION     DAPHJE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.228"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            784..1026
                     /locus_tag="RH222_004395"
     CDS             784..1026
                     /locus_tag="RH222_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDV3799306.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1058..1708)
                     /gene="tetR(A)"
                     /locus_tag="RH222_004396"
     CDS             complement(1058..1708)
                     /gene="tetR(A)"
                     /locus_tag="RH222_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDV3799307.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1814..3013
                     /gene="tet(A)"
                     /locus_tag="RH222_004397"
     CDS             1814..3013
                     /gene="tet(A)"
                     /locus_tag="RH222_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDV3799308.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3045..3905)
                     /locus_tag="RH222_004398"
     CDS             complement(3045..3905)
                     /locus_tag="RH222_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV3799309.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          754 a         1226 c         1198 g          734 t
ORIGIN      
        1 gcacagggcg cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta
       61 aaagacaggt tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct
      121 gcctgtggac agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc
      181 ctcaagtgtc aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata
      241 ccgcagggca cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca
      301 ggcgttttcg ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg
      361 cccctcatct gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc
      421 tcatctgtca gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg
      481 tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag
      541 cccagcggcg agggcaacca gcccggtgag cgtcggaaag gcgctggaag ccccgtagcg
      601 acgcggagag gggcgagaca agccaagggc gcaggctcga tgcgcagcac gacatagccg
      661 gttctcgcaa ggacgagaat ttccctgcgg tgcccctcaa gtgtcaatga aagtttccaa
      721 cgcgagccat tcgcgagagc cttgagtcca cgctagatct atctcatctg cgcaaggcag
      781 aacgtgaaga cggccgccct ggacctcgcc cgcgagcgcc aggcgcacga ggccggcgcg
      841 cggacccgcg ccacggccca cgagcggacg ccgcagcagg agcgccagaa ggccgccaga
      901 gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg
      961 ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg
     1021 ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc
     1081 cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc
     1141 cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca
     1201 gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg
     1261 cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg
     1321 cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca
     1381 tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca
     1441 gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc
     1501 tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg
     1561 cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca
     1621 gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga
     1681 tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat
     1741 gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt
     1801 ccggaggcca gacgtgaaac ccaacagacc cctgatcgta attctgagca ctgtcgcgct
     1861 cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt
     1921 tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttgatgca
     1981 atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt
     2041 gctcgtctcg ctggccggcg ctgctgtcga ctacgccatc atggcgacgg cgcctttcct
     2101 ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc
     2161 cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat
     2221 gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg
     2281 tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac
     2341 gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc
     2401 tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat
     2461 ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt
     2521 cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg
     2581 cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga
     2641 aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt
     2701 cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg
     2761 aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct
     2821 gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac
     2881 ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc
     2941 cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg
     3001 agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg
     3061 caagctatac gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc
     3121 acgagcagga cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct
     3181 agcaacacgg cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     3241 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     3301 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     3361 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     3421 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     3481 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     3541 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     3601 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     3661 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     3721 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     3781 acgagcaata aacccgccgg caacgcccgc agcatcgcga ccgtcatcgg tgagaagttc
     3841 ggcaggtatt gggtggtgac aatgtaggtg ctgccccaaa tggcaggtgc tatcgctgtg
     3901 aacaataaat cg
//
LOCUS       DAPHJE010000226         3761 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPHJE010000226 DAPHJE010000000
VERSION     DAPHJE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.229"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            17..982
                     /gene="lipA"
                     /locus_tag="RH222_004399"
     CDS             17..982
                     /gene="lipA"
                     /locus_tag="RH222_004399"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV3799310.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1083..1286)
                     /gene="tatE"
                     /locus_tag="RH222_004400"
     CDS             complement(1083..1286)
                     /gene="tatE"
                     /locus_tag="RH222_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV3799311.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(1415..2203)
                     /locus_tag="RH222_004401"
     CDS             complement(1415..2203)
                     /locus_tag="RH222_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV3799312.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2296..2679
                     /gene="crcB"
                     /locus_tag="RH222_004402"
     CDS             2296..2679
                     /gene="crcB"
                     /locus_tag="RH222_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV3799313.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(2733..2942)
                     /gene="cspE"
                     /locus_tag="RH222_004403"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(2733..2942)
                     /gene="cspE"
                     /locus_tag="RH222_004403"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV3799314.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3117..3677)
                     /gene="pagP"
                     /locus_tag="RH222_004404"
     CDS             complement(3117..3677)
                     /gene="pagP"
                     /locus_tag="RH222_004404"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV3799315.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          986 a          964 c          870 g          941 t
ORIGIN      
        1 ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga
       61 tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct
      121 gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat
      181 caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa
      241 cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac
      301 ccgccgttgt ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga
      361 accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc
      421 cgttgaccgt gatgacctgc gcgatggcgg tgcccagcac tttgcggatt gcattactgc
      481 cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgtggtcg
      541 tatggatcgt gctctggata ttctgactgc aacgccacca gatgtgttca accataacct
      601 ggaaaacgta ccgcgtattt accgtcaggt acggcctggt gcagattaca actggtcgct
      661 gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat
      721 ggtgggactg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca
      781 tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt
      841 tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg
      901 ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc
      961 gaaagggatg gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc
     1021 cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca
     1081 cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc
     1141 gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg
     1201 cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc
     1261 agtttggtaa tactaatctc acccatagat accttcttga catataaccg gcaacagaac
     1321 gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg
     1381 taacaaggtg aatcaagcgc cgagtagaaa aacatcataa taattgcggc ggcgcaaagc
     1441 gacggttgtt taagacgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt
     1501 ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta
     1561 taatccggct ttgaccgata tttttgttcc cgcactcccc cgccgccacc atataacagg
     1621 tggtatccag cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccgc
     1681 gaacccaggc ggcaggaagt accaggattt cagctccctg taatgcctgt gccagcgcca
     1741 gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca
     1801 gtaacggagc gatttcatta ccagcatcaa cacggcgtga ttcctgaatg gcaaatgcat
     1861 catagagatg cagtttggca taacgggcga cgatgtttcc tgcctgaagt gccaccagca
     1921 tattccatgc gcgccccggc gttgaaggaa catgaatcgt cagaattgtc gtcatcatgt
     1981 tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt
     2041 taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacaaaca
     2101 gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt
     2161 cccacacaga tgtaacagca aactgtcctg cggcaaccag cattgccacc tcctgtcaga
     2221 gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa
     2281 tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga
     2341 agcgtggcga gatggctgtt aagtatgcga tttaacccac tgcatcaggc gattccgttg
     2401 gggacgctga cagcaaacct gattggggca ttcatcatag gaatgggatt cgcatggttc
     2461 agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc
     2521 ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc
     2581 tttggctggg cattactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca
     2641 ctggcattct ggctgttttc ggcctcaacc gcacactaaa gaccaaaaaa aacccgctga
     2701 ttaagcgggt tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa
     2761 gggcctttgg caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta
     2821 aaaccattag tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga
     2881 gtaatgaaac cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac
     2941 atcaaaatta cctttacatg aaaaatagac acaaatgctg tgtcggttac cagtacacca
     3001 attgtggtac gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt
     3061 tgatgttact tattttagct attgatttta aagaagttac taaaacttca tttgcctcaa
     3121 aactgaaagc gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag
     3181 gtcatctgaa aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga
     3241 gggatgtaat tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga
     3301 aaattttcat ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc
     3361 catttgttcc acgagtcctt aaatgccatg gcatacaggc catgccagtt tcctttttca
     3421 tcccaacgcg acaggccaaa accgccaccc cacggtcgct cgttatagcg atcggttttt
     3481 tctttgtcgt aagcgaaacg tgcatgccag gtgatggcag gaatatataa atcataatgt
     3541 tcaggctgtt gccaggtttg tgcaatattt tctctaaacg ttgtcatcca ctcatctgcg
     3601 ttagcaaaaa ctttaccaac gctgattaac tgaataaaaa caaaggaaaa gatagcgaca
     3661 tatttactca cgttcatttg tgaccataaa acatttatca aaaatctact actagcatag
     3721 caaagctaca attaacataa ccttaataga cccaacataa a
//
LOCUS       DAPHJE010000227         3759 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPHJE010000227 DAPHJE010000000
VERSION     DAPHJE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.230"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(357..1079)
                     /locus_tag="RH222_004405"
     CDS             complement(357..1079)
                     /locus_tag="RH222_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HDV3799316.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGIATMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDSELRRKAADELAHMTARINRGEAIPEPVKQLPVIGGKPLSRIQGLAK
                     LAEIREKHGLRRRKQLTGVKLT"
     gene            complement(1076..2095)
                     /locus_tag="RH222_004406"
     CDS             complement(1076..2095)
                     /locus_tag="RH222_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV3799317.1"
                     /translation="MTGSEMAKAGLREQSRLSGANRNALIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKDVYPNQVNTKDKLTKDNIKPYSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(2092..2631)
                     /locus_tag="RH222_004407"
     CDS             complement(2092..2631)
                     /locus_tag="RH222_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HDV3799318.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQIKQAPDHEKIRDAVRAWSAVDN
                     QDVVAALIVNEYREQGDGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMTRLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(2662..2889)
                     /locus_tag="RH222_004408"
     CDS             complement(2662..2889)
                     /locus_tag="RH222_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3799319.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            3000..3692
                     /locus_tag="RH222_004409"
     CDS             3000..3692
                     /locus_tag="RH222_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3799320.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVSHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
BASE COUNT          894 a          909 c          904 g         1052 t
ORIGIN      
        1 taattgaata agtaaaatga aaggatatcg agctatataa tgtagtatgt cgaaaaatca
       61 aacagaaaaa aatcgaatag ttacactgtg ctcggtgctg tcttcatgct ccgttcctct
      121 cgtaggctag taaaattcgc gtcatcaccg ggcttggggg ccatcatcga aaatctgatt
      181 ggtgcttttg tgccaagaga tttcctcttc ggttaccaat cggtagtgaa tcgttcgcta
      241 gatattgctg acatcgacca gatcgccctg ctttgtcaga atgtttacgg tctggttgat
      301 gccgcaggat gaactatatt ttctggggga aaggttttat gtgtcttgaa ggtagttcag
      361 gttagcttca ctcccgttaa ttgtttacgt cttctcagtc cgtgcttctc gcgaatctca
      421 gccaactttg ccaatccttg tatacgggat agcggcttac caccaataac cggaagctgt
      481 ttaactggct cgggaatcgc ctcaccacgg ttaattctcg cagtcatatg ggcaagttca
      541 tcggcagcct tgcgccgtaa ttccgagtca gtcaacgcat tggcccgcat attctggtac
      601 aggttggtaa ccaaccagta gtgcgcgttt gatttccatg gataagactc tgcgtccgga
      661 tacaggccac gcttccggca atactcgtaa accatatcaa ccagctcgct gacgtttggc
      721 agcccggcgg taacggatgc ttcttcccgg caccatgcaa caaactgccc gggtgatggc
      781 agaaatggtc gattctgccg acgggctacg cgcattcctg cgttaacctg ttccattgtg
      841 gcgatcccgt tttcccggaa agccagcacc cactggcggc ggatttcgtt cagttcgttc
      901 tggtcccggt tagccaggct cgccgggaaa gttgccagta actggctgaa cacaccattg
      961 ataatctgcg ctacctgctg tacctgcggc ttttcgtcgt actgttccgg catgttgttg
     1021 gcgatccgac gcatctgctc acggtcaaag ttaatcatct gtgcggcgat gtttttcata
     1081 gatcaacccc gtaaatccag tctgtgtttg tcaggtcgag ttttggtttg ctggctgtca
     1141 cgcctgcctg ttgcttgtta cggttgattt cgagctgggt ccacttgtcg cggagtttgg
     1201 ccggacttag cacgttaccg gaccagaagt tgtcctggca tgcccagcgg aacagcacgc
     1261 acatgtcgcg gtggttacgt ccgtcacgtt cacgcatcag gcggatatcg ttagcccacc
     1321 ctgcaaaatt cggttttctg gctgatggcg cgatggtctt caccatgtca aacatccact
     1381 ctgcggcggt caggtcttct gctgtccccc acttgctgcc gctctgaatc gcagcatccg
     1441 gtttcaccac aggaaggtcg ttttctggct ggtcagagga ttcgccagaa ttctcggacg
     1501 aataaggttt tatattgtct tttgttagtt tgtcttttgt gtttacctga ttcgggtaaa
     1561 cgtctttacc tgatttgggt aaactttttt tacctgattc aggtaaattt acctctttca
     1621 ggtaaacttt atttttctta cctgattcgg gtaatgttga ccattcactg accacattat
     1681 taatgccggt attccgcccg ctctgaataa gaatcccacg ctttaccaga acgctttttg
     1741 cagcagaaca cttgtgcggc aatatcccgg tcaattcgga aagttgctcg ttgctcaccc
     1801 aatccagttt tttattaaag ccatatgttt tgcgcatgac agccaggaag accagaagct
     1861 ggtgctgtgt taatccggcc agcatcacag cttccagcaa ctcatttgca atgcgcgtat
     1921 aaccatcatc gagatctgcc acgcgcggct ccttttgtgc cacatccggc actggaaaat
     1981 tgaatatctc agcagtgttt gccataattc ctcccgcaat gagtgcgtta cgatttgcgc
     2041 ctgaaagtcg gctctgttcc cgcagaccgg ctttcgccat ttctgagcct gtcatattgc
     2101 ccccaacatg gtggtaacca tcgccatcaa tggaccagcc agatccgggt ccacacgaaa
     2161 catcgacaca ataccttcac taatttcctt cagtttctgg tggcgtggtg cgttgagaat
     2221 gacagcctgt tttgcctcac tgagttcctt ttccatttca gccagccgag tcatgaagct
     2281 atcctgctca accaggtaac cgcgatattc cagcggtagt accgccagaa ttgccggggt
     2341 cagttcacgc acgttatttc ggtatttttc agaatcgaat ttgttatcga ggaagcggaa
     2401 cagcttctgg cgtgcacggc tgacatcatc agggaaatcg atggtgccgt cgccctgctc
     2461 ccgatactca ttcacaatga gtgcggcaac gacatcctga ttatctacag ccgaccaggc
     2521 gcggacggca tcacggattt tttcgtggtc tggagcttgt tttatttgag aacgatttat
     2581 caccgcagtc gggctaaatc cgctagtctg ttggtatgta agtggttgca tagtcattgc
     2641 cttatcagtt aacgccgcag tttaggcggc agaattactc gcgttaaaca atggtgcgag
     2701 gtcgggacga atatctgctg gtttaatctt tccaccagtg gctgagacaa ttttcattac
     2761 atagcgggca tcaattccgc caccgtgtag ccaacgccaa actgtgggtt gggctacacc
     2821 gcatagatct gccagtcgtt tttgactacc tgtaatactg attgcgagtt gaatggtttg
     2881 atttgtcatt atcaattcct attggtattg caatgaatga ataatagcaa tgcgtattaa
     2941 tccaagcaat agcaaaacgt gttttgacca tcaatacgca agcgtataaa ttaaaactta
     3001 tgaaaaaaga aactcttgct gatcgcttaa acctagcgat ggaacaatct ggaatgtctc
     3061 aaggcgctct tgcaaaggcg tctggcgtag ctcaacccac aatctggaga ctgacaagcg
     3121 gcaacgcgcg cggctcaaca aaaattgttg aaatagctaa tgcattgggt gttcgaacag
     3181 aatggctctc atcaggcata ggcccgatga gaaatgacgg tcaacaatca gggaagcctg
     3241 ctgtcagcca ttcaaaatac ttcaagattg acgttcttga tatagaagtc agtgccgggc
     3301 cgggtgtaat caaccgtgag tttgtagaag ttctacgctc ggttgagtac tcgtttgacg
     3361 atgctcgtca catgttcgat ggcaggaagg cggaaaatat ccgcatcatt aacgtgcgtg
     3421 gtgacagcat gtcaggaacg atcgaaccag gtgatcttct gttcgttgat atcacggtta
     3481 aatctttcga cggtgatggt atctatgcgt ttctgtatga cgacacagcc catgtaaagc
     3541 gcctgcaaat gatgaaggat aagctgctgg ttatctctga taacaagagc tactcaccgt
     3601 gggacccgat cgagaaagac gagatgaacc gggtgtttat cttcgggaaa gttattggga
     3661 gcatgccgca gacatacaga aagcatggat gacttagcaa gttggtttaa atgtttgtgt
     3721 gataaaccaa actcaaatta taaaaatact tgatgctta
//
LOCUS       DAPHJE010000228         3758 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPHJE010000228 DAPHJE010000000
VERSION     DAPHJE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.231"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..897
                     /locus_tag="RH222_004410"
     CDS             25..897
                     /locus_tag="RH222_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3799321.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTHHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1270..>3758
                     /gene="ag43"
                     /locus_tag="RH222_004411"
     CDS             1270..>3758
                     /gene="ag43"
                     /locus_tag="RH222_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3799322.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETMSGGTLTNHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTASNTAISSGGLQFVGAGGKATDTIINEGGGQSLKGLALNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGYALDTTLNGGNQYVHNGGTASDTVVNSDGWQ
                     VVKDGAVAENTTVNHKGKLQVNAGGVAVDVTQNQGGALVTSTAATVLGSNRLGNFAVE
                     NGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQASNTTIGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTQDAVLSRAVAKGDAPVTFHK
                     LTTNNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRIVAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAG"
BASE COUNT          863 a          965 c         1127 g          803 t
ORIGIN      
        1 tcttttttca ccgggaatac atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa
       61 ccgctctcct ccctgcctta ctcgctttcc cgtcacatcc tggaacatct gcgtaaactc
      121 actcaccacg aacccgtgat tggcattatg ggtaaaagcg gggccggtaa atcctcactc
      181 tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg atgttcacgc cggcacccgg
      241 gaagtgcggc gcttccgtct gagtggacat ggtcacagca tggttatcac tgacctgccc
      301 ggggtgggcg agagccggga cagggatgca gagtatgaag ccctgtaccg tgacattctg
      361 cctgaacttg acctggtact gtggctgatt aaagccgatg accgtgccct gtctgtggat
      421 gagtatttct ggcgacacat cctgcaccgg ggacatcagc aggtactgtt tgtggtgacg
      481 caggccgaca aaacggagcc ctgccatgaa tgggatatgg ccggcattca gccttctccc
      541 gcacaggcac agaatattcg cgaaaaaacg gaggcggtat tccgtctgtt ccggcccgta
      601 catccggttg ttgccgtatc ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg
      661 ctcatgacag cgcttcccga ccatgccgcc agtcccctga tgacccgcct gcaggacgag
      721 ctgcgcacgg aatctgtccg gtctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg
      781 atatttgaca cggcggagag cgtctgtatt gcctctgttg cacgcacggt tctgcgagcc
      841 gtccgtgaca cggtggtctc tgttgcccgc gcggtatgga actggatttt cttctgaaca
      901 gtctgtcagg ggaaatatct gattacattg cgttaaccaa ctgaaaacat gatacaaaca
      961 cctcatgctc ctcctgtttc tcttgcgtga ctgctctgct gttaatagaa taaaacgatc
     1021 gataaaacag gtatcgatcg ttactatcga tcgtttatag tggttaatgg tctgcgaaaa
     1081 ggcagctgaa tctcttcatc atgcagaacg gaattgcaca caacggactg atacttctct
     1141 ggcggatgag agaggggaaa catttatgtc cgggaaaaac cactttatct gaccatgctg
     1201 ttttgtacct gccggtatcc acttttgtgg gtaccggctt ttttattcac cctctgtaag
     1261 gaaaagctga tgaaacgaca tctgaacacc agctacaggc tggtatggaa tcacattacg
     1321 ggcactctgg tggtggcttc cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg
     1381 gttgcgctgt ctcttgctgc tgtcacgtca gttccggccc tggctgctga cacggttgta
     1441 caggcgggag aaaccatgag cggcggaaca ctgacaaatc atgacaacca gattgtcttc
     1501 ggtacgacca acggaatgac catcagtacc gggctggagt atggaccgga taacgaggcc
     1561 aataccggcg ggcaatgggt acaggatggc ggcacagcca gtaataccgc catcagcagc
     1621 ggcggtctcc agtttgtagg tgccggggga aaggctacag acacaataat caatgaagga
     1681 ggcggacaaa gtctgaaggg actggcactg aacaccacgc tgaatggtgg cgagcagtgg
     1741 atgcatgagg gtgcgatagc cacaggaacc gtcattaatg ataagggctg gcaggtcgtc
     1801 aaacccggcg cagtggcaac agacaccgtt gtgaataccg gtgcggaagg gggaccggat
     1861 gcagaaaatg gtgataccgg gcagtttgtt cgcggaaatg ccgtacgtac caccatcaat
     1921 aaaaatggtc gtcagattgt ggctgctgaa ggaacagcaa ataccactgt ggtttatgcc
     1981 ggtggagacc agactgtaca tggctacgcg ctggatacca cactgaacgg cggtaaccag
     2041 tatgtgcaca acggcggtac agcgtctgac actgttgtta acagtgacgg ctggcaggtg
     2101 gtgaaggatg gtgcagtggc tgagaatacc accgttaatc acaagggcaa actgcaggtg
     2161 aatgccggtg gtgtcgctgt tgatgttact cagaatcagg gaggggcact ggttaccagt
     2221 actgctgcaa cggttcttgg cagcaaccgt ctgggcaatt tcgcggtgga aaacggtaag
     2281 gctgacggcg tggttctgga atccggtggt cgcctggatg tactggagag ccattcagca
     2341 cagaataccc tggtggatga cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca
     2401 ggtgtcacca tgacatccgg tggtgccctg atagcagaca gtggtgccac tgttgagggg
     2461 accaatgcca gcggcaagtt cagtattgat ggcatatccg gtcaggccag cggcctgctg
     2521 ctggaaaatg gcggcagctt tacggtcaat gccgggggac aggccagcaa caccacgatc
     2581 ggacatcgtg gaacactgac gctggctgcc gggggaagtc tgagtggcag aacacaactc
     2641 agtaaaggcg ccagtatggt actgaatggt gatgtggtca gtaccggcga tattgttaac
     2701 gcaggggaga ttcgctttga taatcagacg acacaggatg ccgtgctgag ccgtgctgtt
     2761 gcaaaaggcg acgccccggt aacgttccat aaactgacca ccaataacct caccggccag
     2821 ggcggcacca tcaatatgcg tgttcgcctt gatggcagca atgcctctga ccagctggtg
     2881 attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt ttacaaatgt cggaaacagt
     2941 aacctcgggg tggcaacctc cggacagggt atccgggttg tggatgcaca gaatggtgcc
     3001 accacagaag aaggtgcgtt tgccctgagt cgcccgcttc aggccggcgc ctttaactac
     3061 accctgaacc gtgacagcga tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct
     3121 gaagtccccc tgtatgcctc catgctgaca caggcaatgg actatgaccg gatagtggca
     3181 ggctcccgca gccatcagac cggtgtaagc ggtgaaaata acagcgtccg tctcagcatt
     3241 cagggcggtc atctcggtca cgataacaac ggcggtattg cccgtggagc cacgccggaa
     3301 agcagcggca gctatggctt cgtccgtctg gagggtgacc tgctcagaac agaggttgcc
     3361 ggtatgtctg tgaccgcggg ggtatatggt gctgctggcc attcttccgt tgatgttaag
     3421 gatgatgacg gctcccgtgc cggcacggtc cgggatgatg ccggcagcct gggcggatac
     3481 ctgaatctga cacatacatc ctccggcctg tgggctgaca ttgtggccca gggaacccgc
     3541 cacagcatga aagcctcatc ggacaataac gacttccgcg cccggggctg gggctggctg
     3601 ggttcgctgg aaaccggtct gcccttcagt atcactgaca acctgatgct ggagccacaa
     3661 ctgcagtata cctggcaggg actttccctg gatgacggta aggacaacgc cggttatgtg
     3721 aagttcgggc atggcagtgc acaacatgtg cgtgccgg
//
LOCUS       DAPHJE010000229         3751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPHJE010000229 DAPHJE010000000
VERSION     DAPHJE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.232"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            157..2669
                     /locus_tag="RH222_004412"
                     /pseudo
     CDS             157..2669
                     /locus_tag="RH222_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2745..3200
                     /locus_tag="RH222_004413"
     CDS             2745..3200
                     /locus_tag="RH222_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799323.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            3279..3512
                     /locus_tag="RH222_004414"
     CDS             3279..3512
                     /locus_tag="RH222_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV3799324.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWCCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            3612..>3751
                     /locus_tag="RH222_004415"
     CDS             3612..>3751
                     /locus_tag="RH222_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3799325.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TY"
BASE COUNT          926 a          947 c          978 g          900 t
ORIGIN      
        1 tataacggtc aggccacgct gaatgtgacc ttctgacaga accatcgcct ctctgtggtc
       61 ccggtcatca tgaccgggac ccggaccggc gcaacggatc ttcaacgcca cattcgctgg
      121 cattaacaat aacatgatat tcatcacgga gtgactatgt tacagatagt cggcgcgctg
      181 atcctgctga tcgcaggatt tgccattctt cgccttttgt tcagagcatt aatcagcacg
      241 gcttctgcgc tggcagggct catattgctg tgtctgttcg gcccggcctt actggctggc
      301 tatatcaccg aacgcataac ccggttgttc catattcgct ggctggcagg cgtatttctg
      361 acgattgccg gaatgatcat cagcttcatg tggggacttg atggtaaaca tatcgcgctg
      421 gaggctcaca cctttgactc tgtgaaattt attctgacca ctgctctcgc cggtggtctg
      481 ctggctgttc ccctgcagat caaaaacatt cagcagaacg ggatcacacc agaagatatc
      541 agcaaggaaa ttaacgggta ttactgctgt ttttatactg cctttttcct tatggcgtgt
      601 tctgcatgcg caccattgat cgcgttacag tacgatattt caccgtcact gatgtggtgg
      661 ggcgggttgt tgtactggct ggctgcatta gtgacgctgc tatgggcggc cagccagatc
      721 caggcgctga aaaaactgac ctgtgccatc agccagacac tggaagaaca accggtgctc
      781 aacagtaaat cgtggctgac cagtttgcaa aacgattaca gccttcctga ctcactgacg
      841 gagcgcatct ggctgacgct catttctcaa cggatttccc ggggagaggg gaatttgaac
      901 tggcagacgg aaactggtta ctgaacaatg cctggtatga aagaaacatg gcagggttta
      961 acgaacagtt gaaagagaac ctgtcattca cacctgatga actgaaaacg ctcttccgga
     1021 accgcctgaa tttatcaccg gaagcgaatg acgattttct cgatcgttgc ctggacggcg
     1081 gtgactggta tcccttttca gaaggtcgcc gttttgtatc attccatcac gtggatgagc
     1141 ttcgtatctg tgcctcctgc gggctgacag aagtacatca tgccccggaa aatcataagc
     1201 cggatccgga atggtactgc tcctctcttt gtcgcgaaac agaaacactg tgtcaggaaa
     1261 tttatgaacg cccttacaac agctttattt ccgatgcaac ggcgaatggt ctgattctca
     1321 tgaaactgcc ggaaacctgg agtacaaatg agaaaatgtt tgcttccgga gggcagggac
     1381 atgggtttgc cgctgaacgg ggaaaccata ttgtcgacag agtccgtctg aaaaacgcac
     1441 ggatcctcgg tgataataat gccagaaatg gagcagacag actggtcagc ggaacagaaa
     1501 tccagacgaa atattgttca actgcagccc gtagcgtcgg tgcggcattc gacggacaaa
     1561 acggacagta tcgttacatg ggaaataatg gccccatgca actggaagtc ccccgtgatc
     1621 agtatgccgg cgctgttgaa accatgagga ataagatccg cgaaggtaaa gtacccggtg
     1681 taaccgatcc cgcagaagcg tcccggctga ttcgtcgggg acatctgact tatactcagg
     1741 cccgtaatat cacccggttc gggaccatcg aatcggtcac ttatgatatt gccgagggtt
     1801 cggttgtcag tctggcggcc ggagggatca gttttgccct gacggcatcg gtcttctggc
     1861 tcagcaccgg cgatcgcgat gctgccctgc agacagctgc tgtccaggca ggaaaaacct
     1921 tcacccgcac actggctgtc tacgtcacaa cccagcaact ccaccggctc agtgttgttc
     1981 agggtatgct gaagcacatt gatttttcga ctgccagccc gactgtccga ctggctcttc
     2041 agaaggggac cggtgcagga aatatcagtg ccctgaacaa agtgatgaag ggtacgctgg
     2101 tgacatctct ggcactggta gctgtcacaa ccggccctga catgatcaaa atgttgcggg
     2161 gacggatctc cggtacgcag ttcatcagga atcttgccgt ggcatcttcc ggtgtggcag
     2221 gtggagctgt cgggtcagtg gcgggcggga tactgttcag tccactggga ccatttggtg
     2281 cactgacagg gcgtgtggtt ggcggtgttc tggggggaat gattgcctcc gctgtatcag
     2341 gaaaaattgc cggagcgctg gttgaagaag atcgcgtcaa aattctggca atgattcagg
     2401 agcaggtgac atggcttgcc ggcagtttcc tgctgaccgg acatgagatt gaaaatctga
     2461 acgagaatct ggcccgtgtt atcgatcaga atgctctgga gatcattttc gccgccggta
     2521 tacaacaacg ggccgcgacc aatatgttaa tcaaaccact ggtggtcagt atcatcaggc
     2581 aacgccccgt catggaatat gatgcatccc atctcggcaa tatggttaac cgactggaag
     2641 aagcattacc cccggagtta ccggcataac ggctattctg cgccttttac gctttatcac
     2701 ctgatttgtc agtgtcatcg tcatgactca gagaggtaaa taccatgatt cacctgttca
     2761 aaacctgcat gattaccgcc ttcattctgg ggttaacgtg gtctgcccca ctccgggcac
     2821 aggatcaacg ttacatcagt atacgcaata cagatacgat atggctcccg ggaaatattt
     2881 gtgcttacca gttccggctg gataatggcg gaaacgatga aggatttggc cccctcacca
     2941 tcactctgca actcaaagac aaatatggtc agacgctggt gaccagaaaa atggaaacgg
     3001 aagcttttgg tgacagtaat gccacgcgaa ccacagacgc atttctggaa acggagtgcg
     3061 tggaaaatgt cgccacaacc gaaatcatta aagcaactga agaaagtaac ggccatcgtg
     3121 tcagtctgcc gttatcggtt ttcaatcccc aggactacca tccactgctg attaccgttt
     3181 ccggaaaaaa cgttaactga tccctgaagt cagtttcatg cttcagcctc tgtcacaacg
     3241 cgtggcagag gctttttatt tatcaggaga aaacagatat gccgggttac acagagtatg
     3301 tcctggctga aggatctttc tcatacgggc aggcggttgc tgtcataacc gcttaccgca
     3361 atgtgtttat ccaggatgac ccgggaatgc atttccgtcg ggttatccgc aacgcggaag
     3421 gtcagcgacg ctggtgctgc cggaattctg aacccgatgc cggaaaggtg cttaatacgc
     3481 ggctcgccag tgacggtctt ctcagacaat aaacgtcatt catttcaccc atcaggccgc
     3541 gtcttctccg ggagacgtgg ctttttcatt tattacacca gtaaatctta accaccgata
     3601 aggagcaaat tatgcgacta gccagtcgtt ttggttatgc gaaccagata cgccgtgacc
     3661 gtccgctgac acacgaagaa ctgatgcact atgtgccggg gattttcggg gaagataaac
     3721 acacgtcccg aagccagaac tatacgtaca t
//
LOCUS       DAPHJE010000230         3606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPHJE010000230 DAPHJE010000000
VERSION     DAPHJE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.233"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..133
                     /locus_tag="RH222_004416"
     CDS             <1..133
                     /locus_tag="RH222_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799326.1"
                     /translation="NIIPIITATLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            135..479
                     /locus_tag="RH222_004417"
     CDS             135..479
                     /locus_tag="RH222_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV3799327.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            483..818
                     /locus_tag="RH222_004418"
     CDS             483..818
                     /locus_tag="RH222_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV3799328.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(874..3195)
                     /locus_tag="RH222_004419"
     CDS             complement(874..3195)
                     /locus_tag="RH222_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3799329.1"
                     /translation="MRGNNTIVTTGDYSVGLLSQVDGNLNTDTIIRVNPDGSATPSFS
                     DGDNTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTENNENSAKAILDMAKGE
                     ITTHGTESYAAYANGTVVKAGDKLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGAGVVLESSINGQEATVDILSGSSL
                     RSANEILYNKNETSNVTITDSEVSSAADVFINNIKGHLTVDATDSKITGSANLSTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDFEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPIVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSSG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWLHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT          936 a          875 c          788 g         1007 t
ORIGIN      
        1 aaacataatt cctatcatca cagccacgct cattttgaat tatctgaata atagcgagcg
       61 ttctttagtt aagcagatct taattgaaga cgaaattatt gtgtgtgcaa cttacttaat
      121 accggacatc taagttgaag aaaaaaatac ttgccttcgg gcttatctca gcattgttct
      181 gctccacgcc agcgatggcg gatatgaatc gcaccacgaa aggtgcattg ctaggcgcag
      241 gcgttggttt acttactggc aatggtgtta atggcgtact gaaaggtgca gctgttggcg
      301 ctggtgttgg tgcagtaaca gaaaaaggcc gcgacggtaa aaatgcgcgt aaaggtgcaa
      361 aagtaggtgc cgccgttggt gctgtcactg gcgttctgac cggcaatggc ctcgaagggg
      421 cgattaaagg tgccgtgatt ggcggtacgg gtggtgcaat tctgggtaaa atgaaataag
      481 gaatgatgat gaaaaaattt gctttactgg cgggcctgtt tgttttcgca ccaatgacct
      541 gggcgcagga ctacaatatt aaaaacggtt tgccgtcaga aacctatatt acttgcgcag
      601 aagccaacga aatggcaaaa actgacagcg cacaggttgc agaaattgtt gcggtaatgg
      661 gtaatgccag cgttgccagc cgtgatttaa aaattgagca atctccggaa ctgagcgcca
      721 aagtggtaga aaaactgaat caggtttgcg ctaaagaccc gcaaatgctg ttgatcacgg
      781 caattgatga caccatgcgt gctatcggca aaaaataata tttaatatgt taccgcgtcg
      841 atgaattcga cgcggtattt tagctaacgg ggatcagaac ttatatttca ggcccaccat
      901 cactgcggtg tcattgtagc cattatcacc cagctgcacg cccacattac cccagacgct
      961 ggccgccgga ttcagattgc catttacgcc aagtttaatt tcccccaaat tacgcgcccc
     1021 gtcttgttcg actttcacac cgttcattga gacagcaaaa tctttactgt tatggagcca
     1081 gttcatttca acaaaaggtt caaagtcgtc accgtgcgga ccgctgtttt tctcctgagt
     1141 acgaataaag gtgcggaaac caagtcgggt ctggatgttg ttatttgcgt cattctcaat
     1201 acgtgagcca ttggcttccg tgtgcgcatt atgatcgacg cccatccaga tagcctgtgc
     1261 ctgtggctgg aagatccacg tatatttcgc cgcttccaga ccaaaggatt tattcagggc
     1321 aaagccgtaa cccgcttcca gcgagacggt tgcgcctttc gcgctatagg attcataggc
     1381 taattcatcc cctttcaccg agtttttaaa ccagctatat tgcgcccagg cgtcaatgta
     1441 tgcgcctttt ttactgatgt catcggcaaa ccaggtggca tacagccctg cgctatagcc
     1501 tctgacactc cctttcgaac gataatccga cacgctggaa tgagttgagt tgtagtcgcg
     1561 ggcataacca gccatcactc ccaaacgcca actgtcactg tcggtaaaac caccggttaa
     1621 cagatcggcc cctaactgcg agacgtagcg atgcgaggtc gttctcaact gtccgctgct
     1681 gtcacgccag gcattatgcc cgccaatttg acgtagccaa agtcggcttg aacgctcctg
     1741 ttcagttaca ggatcgatgt aacgcgtttc acctgcacga tcgttcagac gcataacaaa
     1801 aagagtgtta gcggctgcca ggttggcgac gtaacttcca gcttcggggc gcaggacggg
     1861 ggtaggcgca acaataggcg ctgaacttcc tccggagttt gtttcaccgc cgctcgttgc
     1921 ctggaagtta gtcagatacc agtttttatt ggtcgcttcg gtattacctc gggttaatga
     1981 atattcgtag gcaccggcga aaatcctcga atccttaata aattctccat ttgattcccc
     2041 ctcaacgctg ataatctcta tcccatttaa cgtgtaagcc ccgctgccgc cagcattggt
     2101 aactttaact cgggttgttc cagaggtatt tccattaata acgactttat ccgtagctga
     2161 attatcatca cccaattcag ttctgagatg caatacgcca ttattaccaa cgtaattttc
     2221 agttattgtt aatcttgttg gttcgaaatc ccttccatct gcccgggaaa tatagaccgt
     2281 actattgtca accgtgaggt tgctcaccgt tgagtcagct ttaatatccc aggtactatt
     2341 atctgaaagc gacagatagg tatgagtgtt atcatctgtt gaaagattgg ctgaacccgt
     2401 tatttttgaa tcagttgcat cgacggtcaa atgaccttta atattattga taaaaacatc
     2461 tgcagccgat gatacctcac tatcagtaat ggtcacgtta ctcgtctcat ttttattgta
     2521 gaggatttca tttgctgacc gtagtgagct accagataaa atatctaccg ttgcttcctg
     2581 accatttatg gaagactcca gcacaactcc agccccctga tgcgcgactg cagatgtatt
     2641 tttcagcgtc accgtgccgc cattataaat tttagcaatc gcggcgtcag gacccgttgt
     2701 ataaatttct ccctggttaa atgaaactgt cccctggcga gcagcgatgg catgagcatt
     2761 gtccccatgc gtggtgatat caacatcggt taatgtaacg ctggcattgg tataatcaag
     2821 cttgtcacca gccttgacga cagtgccatt agcgtaagcg gcataacttt ctgttccgtg
     2881 ggttgttatc tccccttttg ccatatccag tattgctttg gcgctatttt cattattttc
     2941 tgtatcggca gtactttctt catcaagaga agatacacac gcacgagcgc ttccgggaga
     3001 tgcacacgca agaacgccga ctgcgtgatt acccgcagta acaataaaag tattatctcc
     3061 gtcagaaaaa gagggggtag ccgaaccgtc agggttgact cttattatgg tatcagtatt
     3121 caggtttcca tcaacttgac ttaacaaacc aacagaataa tctcccgtcg tgacaatcgt
     3181 attattaccc cgcaaagtga cagcaccgcc atcttcactt acgattgcac tagctgttaa
     3241 tgctgtgctt agagaactat tgtcagaagg atataaaccg cccgttgtta caatgacgcc
     3301 attgttaatg gttaaattgc tacccactcc tgcaacggat accccgtaac ttccattacc
     3361 cgttgttata actagcgcat tatcaatata ttttacttct ccacctttta cttctccatt
     3421 ttttacttct ccatctttta ttgccacagc atgtttatgt tcctcgtgct gaataaactg
     3481 ttcagcatat gcattaccca ccgccagagc aataagtcct aaactaaacc cggggagttt
     3541 tttcagtttc atagattaac catccattca ttggactaac aaacattcct ggtcaaataa
     3601 cctatg
//
LOCUS       DAPHJE010000231         3546 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPHJE010000231 DAPHJE010000000
VERSION     DAPHJE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.234"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..184
                     /gene="rrf"
                     /locus_tag="RH222_004420"
     rRNA            69..184
                     /gene="rrf"
                     /locus_tag="RH222_004420"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(288..815)
                     /gene="mobB"
                     /locus_tag="RH222_004421"
     CDS             complement(288..815)
                     /gene="mobB"
                     /locus_tag="RH222_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV3799330.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(797..1381)
                     /gene="mobA"
                     /locus_tag="RH222_004422"
     CDS             complement(797..1381)
                     /gene="mobA"
                     /locus_tag="RH222_004422"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV3799331.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1451..1720
                     /locus_tag="RH222_004423"
     CDS             1451..1720
                     /locus_tag="RH222_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV3799332.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1797..2783
                     /gene="srkA"
                     /locus_tag="RH222_004424"
     CDS             1797..2783
                     /gene="srkA"
                     /locus_tag="RH222_004424"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV3799333.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2800..3426
                     /gene="dsbA"
                     /locus_tag="RH222_004425"
     CDS             2800..3426
                     /gene="dsbA"
                     /locus_tag="RH222_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV3799334.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          916 a          848 c          863 g          919 t
ORIGIN      
        1 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
       61 agaatttgcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg aactcagaag
      121 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
      181 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcatt ggcgcagaaa
      241 aaaatgccgg atgcgacgct ggcgcgtctt atccagccta ctcttgctta tccgttctgc
      301 ttttgcatcc actccaccac aaaatcagcc agcccctcaa catcattaat atccagtaat
      361 gcgacatcaa gattaagcgg cacatcactg gctacagcaa taacatgcct gtctatcact
      421 aattcttcag gtcgatgtcc ggctccatcg cgaaacagca caatctttgc gatctcttca
      481 tgcttaaacc cttcgaccag aatcaaatcc agctttgagg tatccattcg acttgcgaga
      541 aactgtagat ccagctcttc ttcgtctggt gtttccgtca tcaaggccca tcgttgctgg
      601 ctggcaacga tggtttgtgc cgcgccagcc ttgcgcagct catagctatc tttgcctggc
      661 ttatcaacat ccatatcatg gtgcgtatgc ttaatcagcc ctggacggat ccctctggcg
      721 cataatgccg ggatcagttt tttcaaaagc gtagtttttc cggtaccact ccacgcggca
      781 aaggcgagta acggtatcat cgtttttcct gccatcgggc aagctcctct ggcgtattca
      841 cgttaacaaa tgcatcttta tgatcgctga aatcaaccgc atgaccgcca gccagacgca
      901 taaataccat tacccggcgt tctcctgctt gcagatattc cagtaataaa ggctcaatag
      961 cgcggtttac cagagcaata gtcgggtgat cgcgttcacc gtcatggacc cacacgacag
     1021 gcgcatcttt gcgctgatga ttaagcctgg ctgctaaatc agggggaatg taaggcgtat
     1081 cgcacgggca aaacaaaaac cactcaccag cttcctgctg cattactgaa agcattcctg
     1141 ccagagggcc tgggtaatcc gccagtgaat cttcaatcac tttcagaccg cttgcctgat
     1201 agatttcctg atgacgatta gcattaacca cgacgtgaga gagctgcgtc ataagcgcgt
     1261 cagcgacatg ttgccataat ggtttgccgt ttaattcaag caatccttta tctacgccgc
     1321 ccattcgtct ggctttaccg cctgccagca caacgcctgt tatcgtcgtc atcagattca
     1381 ccgatatcgc ctcttttatt gtgggattga ccctgctaac gtgtctgtct caagagtaag
     1441 gagcatcact atgaaatgta aacgtctgaa tgaagttatt gaactcctcc agccagcctg
     1501 gcaaaaagag ccagacctta acctgctgca atttttgcag aaactggcga aagagtcagg
     1561 ttttgacggc gaactggcgg atttgacgga tgacattctg atctatcacc tgaaaatgcg
     1621 tgattccgcg aaagatgcgg tgatcccggg tttgcagaaa gattatgaag aagacttcaa
     1681 aacggcgctg ttacgcgctc gcggcgtaat taaagagtaa aagcttgtaa gcggcgccac
     1741 caaaatcatc gtgaaatgat atccttcgtc attcgtaatg ttttccggat gatgggatga
     1801 ataacagcgc ttttactttc cagacactac acccggatac catcatggac gctctgtttg
     1861 agcatgggat ccgggtggat tccggtctta ccccgcttaa cagctatgaa aaccgtgtct
     1921 atcaatttca ggacgaagat cgtcgacgtt ttgtcgtcaa attttatcgc cctgaacgtt
     1981 ggacagccga tcaaatcctc gaagaacatc aatttgcgtt gcagctggta aatgatgaag
     2041 ttccggtcgc agcacctgtg gcctttaacg gtcagacttt attgaatcat cagggatttt
     2101 atttcgctgt ttttccaagc gtcggtggtc gccagttcga agctgataat atcgatcaga
     2161 tggaagcggt tgggcgttat ttagggcgta tgcaccagac ggggcgcaaa cagcttttta
     2221 tccatcgccc gaccatcggt ctgaatgaat atctcattga gccacgcaag ctgtttgagg
     2281 acgctacact gatgccttcc gggttgaaag cggcattcct gaaagcgaca gatgagctga
     2341 tcgccgccgt tacagcacac tggcgggaag acttcaccgt tctgcggcta catggagact
     2401 gccacgccgg gaatattctc tggcgcgatg gtccaatgtt tgttgatctg gatgatgcac
     2461 gtaatggtcc agctattcag gatttgtgga tgttgctcaa tggcgataaa gccgagcagc
     2521 ggatgcaact ggaaactatt attgaagctt atgaagaatt tagcgagttc gacaccgctg
     2581 aaatcggact gattgaacct ttacgcgcca tgcgtttggt ttattatctt gcctggctaa
     2641 tgcggcgttg ggctgatccc gcgttcccga aaaatttccc gtggttaacc ggggaagatt
     2701 actggctgcg acaaacggcg acttttatag aacaggcaaa agttctacaa gaaccccctt
     2761 tacaattaac acctatgtat taatcggaga gagtagatca tgaaaaagat ttggctggcg
     2821 ctggctggtt tagttttagc gtttagcgca tcggcggcgc agtatgaaga tggtaaacag
     2881 tacactaccc tggaaaaacc ggtagctggc gcgccgcaag tgctggagtt tttctctttc
     2941 ttctgcccgc actgctatca gtttgaagaa gttctgcata tttctgataa tgtgaagaaa
     3001 aaactgccgg aaggcgtgaa gatgactaaa taccacgtca acttcatggg tggtgacctg
     3061 ggcaaagatc tgactcaggc atgggctgtg gcgatggcgc tgggcgtgga agacaaagtg
     3121 actgttccgc tgtttgaagg cgtacagaaa acccagacca ttcgttcagc atctgatatc
     3181 cgcgatgtat ttatcaacgc aggtattaaa ggtgaagagt acgacgcggc gtggaacagc
     3241 ttcgtggtga aatctctggt cgctcagcag gaaaaagctg cagctgacgt gcaattgcgt
     3301 ggtgttccgg cgatgtttgt taacggtaaa tatcagctga atccgcaggg catggatacc
     3361 agcaatatgg atgtttttgt tcagcagtat gctgatacag tgaaatatct gtccgagaaa
     3421 aaataattaa atgtaaatta cataaaaccc gtgaatattc acgggctttt tttattattt
     3481 aataaatata aatacattct gataatgcat cctgccgctg gacattattt cagcataagc
     3541 ggatat
//
LOCUS       DAPHJE010000232         3463 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPHJE010000232 DAPHJE010000000
VERSION     DAPHJE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.235"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2149
                     /locus_tag="RH222_004426"
     CDS             <1..2149
                     /locus_tag="RH222_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV3799335.1"
                     /translation="KAAKTSETNARSSETAAEQSASAAADSETAAASSAREASIKAEE
                     AAVSATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETSAE
                     SSKTAAASSASSAASSASSASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQS
                     KSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAV
                     KAAYDLANGKYTAQDATTAQKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQ
                     DATTAQKGIVQLSSATNSTSETLAATPKAVKAANDNANGRVPSARKVNGKALSADITL
                     TPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISE
                     LVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYT
                     SNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQ
                     GQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASS
                     TDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYG
                     YTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSV
                     AIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2149..2724
                     /locus_tag="RH222_004427"
     CDS             2149..2724
                     /locus_tag="RH222_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3799336.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(2822..3412)
                     /locus_tag="RH222_004428"
     CDS             complement(2822..3412)
                     /locus_tag="RH222_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3799337.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          949 a          837 c          892 g          785 t
ORIGIN      
        1 gaaggcggca aaaacgtccg agacgaatgc caggtcttct gaaacggcag cggaacagag
       61 cgcctcagct gcggcagact ctgagacagc agctgcatca tcggcccgtg aggcgtccat
      121 taaggcagag gaggcagcag tcagtgccac cgccgccgga aaatcggcag aaagcgccgc
      181 atcatccgct tcaacagcca caacgaaggc tggcgaagcc actgaacagg ccagcgcagc
      241 agcgaggtct gcttccgcag cgaagacatc cgaaacgaac gcgaaagcgt cggaaaccag
      301 cgcagaatcc tcaaaaacgg ctgccgcatc gtccgccagt tcggcggcgt catcggcatc
      361 atctgcgtct gcttcaaaag atgaggcgac cagacaggcg tcagcagcga ggggcagcgc
      421 cacgacggca tccacgaagg cgacagaggc tgctggcagt gcggcagcgg cagcacagag
      481 caaaagtacg gcggaatccg cggcaacgcg cgccgagaca gcagcaaaac gggcagagga
      541 tattgcatcc gccgtggcgc ttgaggatgc gagcacgacg aaaaagggga tagtacagct
      601 cagcagtgcg accaacagca cttccgagtc acaggcggca acgccaaaag ccgttaaggc
      661 cgcgtatgac ctggctaacg ggaaatacac cgcacaggat gcaacgacag cacagaaagg
      721 gatagttcag ctcagcagtg cgactaacag cacttccgag tcacaggcgg caacgccaaa
      781 agccgttaag gccgcgtatg acctggctaa cgggaaatac accgcacagg acgctacgac
      841 agcacaaaaa ggaattgtcc agctcagtag tgcaaccaac agcacatctg aaacgcttgc
      901 tgcgacaccg aaagcagtga aagcagctaa tgacaatgcg aatggtcggg taccttctgc
      961 ccgtaaggtg aatggtaagg cgctttcagc ggatataaca ctgacgccga aagatattgg
     1021 tacgcttaac tcaacaacaa tgtcattcag cggtggtgct ggttggttca aattagcaac
     1081 ggtaaccatg ccacaggcga gttctgttgt ttcaattacg ttgattggtg gcgctggatt
     1141 taacgtgggg tcacctcaac aggcaggtat atctgaactt gttttacgtg caggtaatgg
     1201 taatccgaag gggattactg gtgctttatg gcagcgcaca tcgacagggt ttacaaattt
     1261 tgcctgggtc aatacatctg gtgatactta cgatatttac gttgcaatcg gaaattatgc
     1321 gactggtgta aatattcaat gggattatac cagtaatgcc agcgtgacga ttcatacgtc
     1381 accagcatat tctgctaata agccggaagg gttaacggac ggtacagttt attcactcta
     1441 tacgccatca gagcagtttt atccgcctgg cgcaccaatc ccgtggccat cagataccgt
     1501 tccgtctggc tatgccctga tgcaggggca gacttttgac aaatctgcat acccgaaact
     1561 tgcagccgct tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa
     1621 acctgccagt ggtcgggccg tattgtctca ggaacaggac ggcattaaat cgcataccca
     1681 cagcgccagc gcatccagta cggatttggg gacgaaaacc acatcgtcgt ttgattacgg
     1741 cactaaatcc acgaataaca ctggtgcgca tacccatagt ttaagtggca gcacgaatgc
     1801 agctggtaat cacagccata gagatggccg tcgatttaac cccagtgttt ttaaagatac
     1861 ttatcaatat ggttatacaa gctcaggtca aaatacctgg ggtgtacaag gctcagtagg
     1921 tatgtctacg gggtggttag cgaataccag tacagatggt aatcatagcc attcactgtc
     1981 cggcacagca gcatctgcag gtgcacacgc gcatactgtc ggtattggtg ctcatacgca
     2041 ctccgttgcg attggttcac atggacacac catcaccgtt aacgctgctg gtaacgcgga
     2101 aaacaccgtc aaaaacatcg catttaacta tattgtgagg cttgcataat ggcattcaga
     2161 atgagtgaac aaccacggac cataaaaatt tataatctgc tggccggaac taatgaattt
     2221 attggtgaag gtgacgcata tattccgcct catacaggtc tgccagcaaa cagtaccgat
     2281 attgcaccac cagatattcc tgctggcttt gtggctgttt tcaacagtga taaggcatcg
     2341 tggcatctcg ttgaagacca tcggggtaaa acggtttatg acgtagcgtc aggggacgcg
     2401 ttatttattt ctgaactcgg tccgttaccg gaaaatgtta cctggttatc gccggaaggg
     2461 gagtttcaga agtggaacgg cacagcctgg gtgaaagatg cagaagcaga aaaactgttc
     2521 cggatccgag aggcggaaga aacaaaaaac agcctgatgc aggtagccag tgagcatatt
     2581 gcgccacttc aggatgctgt agatctggaa atcgcaacgg aggaagaaac ctcattgctg
     2641 gaagcctgga aaaagtatcg ggtgttgctg aaccgtgttg atacgtcaac tgcacaggat
     2701 attgaatggc cagcactgcc gtagggtaaa acatataaat tctataatta gatgtatctt
     2761 tccatttacg gcaaggaagg gggcttggaa gacgtaaagc atctcacacc gagattattt
     2821 tttatatgtc aggtgtctga agttttgctt tggctcttaa aatggtttgc cgcgaggttt
     2881 tgaattcccg ggcaatggca cttatactta cacctgactt aattcgttcg aataccacct
     2941 gtttctgttc ttcatttaac acaggtggtc gaccaaaacg tttccctgcg ccgcgggctc
     3001 ttactatccc ggaatgagtg cgttcaagta aaaggtctcg ttcaaattca gcgactgctg
     3061 aaattacgtg catcatcatt tttcctgttg gactggtcag gtcaatgccc cccaatgcta
     3121 agcaatgcac tctgatacct gtttcggtca gttgttccac tgttttcctg atatccattg
     3181 cattacaacc aaggcgatcc agttttgtca caatcaattg atcaccacat ttcaggcgag
     3241 caagcaaccg gttaaaacca ggacgctcac tggttgctgc tgagccgcta atgtgttctt
     3301 cgattatttg ctgaggtttg attttaaaac ctgcactttc gatttcccgg cgttgatttt
     3361 cgatggtctg atccagcgtt gatatccgac agtaagcaaa aattcgagac atagtgagac
     3421 tctatacgaa attggtgttc atatcataat gcatctcaga aaa
//
LOCUS       DAPHJE010000233         3453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPHJE010000233 DAPHJE010000000
VERSION     DAPHJE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.236"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..656
                     /locus_tag="RH222_004429"
     CDS             39..656
                     /locus_tag="RH222_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3799338.1"
                     /translation="MTDACEGCVRFIDIPCHIISDYLYIRLRDADALCITDRKNSFFR
                     KKAYGTFLNSLMSLAEQQKNTNLYESVLFVLLDYLNDKNQLLPVLSGGLNNYSLRVEA
                     IVAAEPAKKWYLSDVASALCISASQLKRKLHSEGAGFSRIITEVRMKKAITLMKCGNA
                     NIFIVARECGYSSLSYFILSFRKYYSVTPCQWLKQYGIKDTTGDG"
     gene            899..2128
                     /locus_tag="RH222_004430"
     CDS             899..2128
                     /locus_tag="RH222_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3799339.1"
                     /translation="MSYPVQKKIKTGENVLRAAVERINWVFDTFAEICLSFSGGKDST
                     VLFHLVADIARRKKRRFSVLFIDWEAQYLCTVRHVQKMRELYMDVTESFYWVALPLTT
                     VNGVSQFQPEWICWESGVEWVRQPPDYAVTDPNFFPFYQYAMTFEEFIPAFSSWMARS
                     KRGLAVLTGVRADESLNRFMGLASQHKLRYADDKPWTTASQDGFYYTMYPLYDWKTRD
                     IWIYNAKTHAIYNPLYDLMFRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMC
                     ARVSGAASGALYANESGAYFALRKRISKPDHHTWRSYAMFLLNVMPEKTAEHYRNKIA
                     VYLHWYQTHGYPDDIPDEQDNDLGSRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHY
                     ERYLQRMKERRKEWGIL"
     gene            2113..2751
                     /locus_tag="RH222_004431"
     CDS             2113..2751
                     /locus_tag="RH222_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV3799340.1"
                     /translation="MGDPLTPEILTVMIRQYFCQHLSEEETINALNHLRRVLHEISPF
                     AREPVDCVLWVKSDEVIANDYNPNVMAPGEKKLLKQSLEQDGFTQPVVVSEEKDHYLV
                     VDGYHRQLLGRGAGTEKRLRGWLPVTCINPERKGQATRMAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWSDERIGTELGMDQDEVLRLKQISGLTELFQDEDFSPAWTVR"
     gene            2736..3092
                     /locus_tag="RH222_004432"
     CDS             2736..3092
                     /locus_tag="RH222_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV3799341.1"
                     /translation="MDGSLIQLNKILVDEFLSTQKRALEAVDDLIALKLEAAGCWRRA
                     SARWLVVMGAGDITDAQREWLLRRRAYCMAQTTSHVLNEKMNIRGVAKAADETLKRMG
                     IADLSEEMFRKRPSYY"
BASE COUNT          929 a          657 c          891 g          976 t
ORIGIN      
        1 atgaaattct ctttcttaaa agaaatgaca ttcagtatat tacggatgcc tgtgaaggat
       61 gtgtgaggtt tattgatata ccctgtcata tcatcagcga ctatctgtat atcagactga
      121 gggatgccga tgcgttatgc atcactgaca ggaaaaattc ttttttcaga aagaaagcgt
      181 atggtacctt tctgaactcg ctgatgtcac ttgcggaaca acagaaaaat actaaccttt
      241 atgagtcagt attatttgtg ctgctggatt atcttaatga taaaaaccag ttgctgcctg
      301 tattgtccgg tgggctgaat aactactcgt tacgtgttga agctattgtt gccgcagagc
      361 cggcaaagaa atggtatctg tcagatgttg catcagcgtt atgtataagt gccagtcagc
      421 ttaaaagaaa actgcacagt gaaggcgcag gattcagccg gataatcaca gaagtacgta
      481 tgaaaaaggc aataactctg atgaagtgcg ggaatgctaa catttttatt gtcgccagag
      541 agtgcggata tagcagtctg tcgtatttta ttttaagttt cagaaaatac tactcagtca
      601 ctccctgtca gtggttaaaa cagtatggta ttaaggacac aactggtgac gggtaaaccc
      661 tgattcacat aaaccggttt tctggcgtat acatgctgta cagtcatcca ggccttataa
      721 ggggtatatt gattgttttg tgatatgttt caattttttg ttgttgttat tgttatattt
      781 tttaaaaaat cttactcatt gtgaaacgat ttttatcgtg cactgtgctt gattacatgg
      841 ttaccagagg tgtctctgtg taagtgatgt ttctgtatgt ttattacatt gagaacaaat
      901 gagttatccg gtacagaaaa aaataaaaac aggtgaaaat gttctccggg ctgctgtgga
      961 acggattaac tgggtttttg atacttttgc ggagatttgt ctgtcttttt ccggaggtaa
     1021 ggattccact gttttatttc atctggttgc ggatattgcc cgcagaaaaa aacgacgttt
     1081 ttccgtgttg tttatcgact gggaagcgca gtatttatgt actgtccgcc atgtacaaaa
     1141 aatgcgtgaa ttgtatatgg atgtcacaga gtctttttac tgggtggccc tgcctctgac
     1201 aacggtgaat ggggtttccc agttccagcc tgaatggata tgctgggagt ctggagtgga
     1261 atgggtccgt cagccacctg attatgctgt tactgacccg aactttttcc ctttttatca
     1321 atatgccatg acatttgaag aattcattcc ggctttttca tcctggatgg ccagaagtaa
     1381 acgtgggctg gcagtgctga ccggagttcg tgcagatgag tccctgaaca ggtttatggg
     1441 gctggcttcg cagcataagc tacgctatgc ggatgataaa ccatggacga ctgcatcaca
     1501 ggacggtttt tactacacga tgtaccctct gtatgactgg aaaacccggg atatatggat
     1561 atacaacgcg aaaacgcatg ccatttataa ccccctgtat gacctgatgt tcagagctgg
     1621 cattccatta cggaacatgc gtgtctgtga accctttggc cctgaacagc gtaagggatt
     1681 atggctttac catgttctgg agcctgaaac ctgggccaga atgtgtgccc gggtctcagg
     1741 agcagccagt ggagcgctgt atgcaaatga aagtggtgct tattttgcgc ttcgaaaacg
     1801 tatatcaaag cctgaccatc atacctggcg cagctatgcg atgtttttac tgaatgtgat
     1861 gccggaaaaa acggcggaac attaccggaa caaaatagcc gtatatttgc actggtacca
     1921 gacacatgga tatcccgatg atattcctga tgagcaggat aatgatctgg ggagtcgtga
     1981 tgttccgtca tggcggcgta tatgtaagac gctcataaag aatgatttct ggtgccggac
     2041 cctgtccttc agcccaaaca aaccccgtca ctatgaacgt tatctgcaaa gaatgaaaga
     2101 gaggagaaag gaatggggga tcctttaacg ccggaaatac tgacagtcat gatccgtcag
     2161 tatttctgtc agcacctgtc agaagaagag acaattaatg cgctgaatca tctgcgcagg
     2221 gtgcttcatg agatcagccc ttttgcccgg gaaccggtgg actgtgtgtt atgggtgaaa
     2281 tcggatgagg tgatagccaa cgattataat cccaatgtca tggcgccggg tgagaagaaa
     2341 ctgctgaagc agtcactgga gcaggatggg tttacacaac cagtcgtggt atcggaagaa
     2401 aaagatcatt atctggtggt ggatggttat catcgtcagt tgctgggaag aggggccggt
     2461 acagaaaaac gcctccgggg atggcttcct gttacctgca ttaatccgga gagaaagggg
     2521 caggcgaccc gtatggccgc gaccatccgt cataaccggg ccagaggaaa gcatcagatt
     2581 acatcaatgt cagatatcgt tcgtgacctg tcccgtctgg gatggtcgga tgaaagaatt
     2641 ggtactgagc tgggtatgga ccaggatgaa gtgctgaggc tgaaacaaat cagcggactg
     2701 actgagctgt ttcaggatga agatttcagt ccggcatgga cggttcgtta attcaattaa
     2761 ataaaattct ggtggatgag tttttgagta cgcagaagag agcactggaa gcagtggatg
     2821 atctgatcgc actgaagctt gaggctgcag gatgctggcg cagggcttct gcacgatggc
     2881 tggtcgtgat gggggcgggc gatattacag atgcacaacg ggagtggctt ttacgacgac
     2941 gggcatattg catggcgcag accacttctc atgttttaaa cgaaaagatg aatatccggg
     3001 gggtggcaaa agctgcagat gagaccctga agcgtatggg aattgcagat ctgtcagaag
     3061 agatgttcag aaaaagacca tcatactact gataacgggc tctctgtggc tggcttctga
     3121 agtggaatat atgtgaaaga gaccggatat attgcgcttt ttactttcaa taaatcagta
     3181 aacaggattt gcgatgtatt atttatcata atataacaaa ttgtaatcag tcagagtctg
     3241 cagactttgt caggcttcag atatcttatt gtggatttct gtatgtttgt agacgttttt
     3301 tcggaaagac atgctgtgga cagcaagtgt tgtatgtagc cctcgaattg gatgcaatct
     3361 ttgttgctgc atttctgatg cgcgtttctg gcatatgatt tgccggactg tcccttcgac
     3421 ctgaaaagat gccagaaacg cgactgtttt att
//
LOCUS       DAPHJE010000234         3400 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPHJE010000234 DAPHJE010000000
VERSION     DAPHJE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.237"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..1085
                     /gene="dusB"
                     /locus_tag="RH222_004433"
     CDS             120..1085
                     /gene="dusB"
                     /locus_tag="RH222_004433"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV3799342.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1111..1407
                     /gene="fis"
                     /locus_tag="RH222_004434"
     CDS             1111..1407
                     /gene="fis"
                     /locus_tag="RH222_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV3799343.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="RH222_004435"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="RH222_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV3799344.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="RH222_004436"
     CDS             2461..2640
                     /locus_tag="RH222_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV3799345.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="RH222_004437"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="RH222_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV3799346.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          932 a          782 c          807 g          879 t
ORIGIN      
        1 ttttaaatgc aattctttga tccatctcag aggattggtc aaagtttggc ctttcatctc
       61 gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag tatggtcatt tcttaactca
      121 tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc agcgcccatg gctggcatta
      181 cagacagacc ttttcggacg ttgtgctacg agatgggagc cggattgaca gtatccgaga
      241 tgatgtcttc taacccacag gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca
      301 ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag cgatccgaaa gaaatggcag
      361 atgcagcacg tattaacgtg gaaagcggtg cccagattat tgatatcaat atgggttgcc
      421 cggctaaaaa agtgaatcgc aagctcgcag gttcagccct tttgcagtac ccggatgtcg
      481 ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt tcctgttacc ctgaagattc
      541 gcaccggctg ggcaccggaa caccgtaact gcgaagagat tgcccaactg gctgaagact
      601 gtggcattca ggctctgacc attcatggcc gtacacgcgc ctgtttgttc aatggagaag
      661 ctgagtacga cagtattcgg gcagttaagc agaaagtttc cattccggtt atcgcgaatg
      721 gcgacattac tgacccgctt aaagccagag ctgtgctcga ctatacaggg gcggatgccc
      781 tgatgatagg ccgcgcagct cagggaagac cctggatctt tcgggaaatc cagcattatc
      841 tggacactgg ggagttgctg cccccgctgc ctttggcaga ggttaagcgc ttgctttgcg
      901 cgcacgttcg ggaactgcat gacttttatg gtccggcaaa agggtaccga attgcacgta
      961 aacacgtttc ctggtatctc caggaacacg ctccaaatga ccagtttcgg cgcacattca
     1021 acgccattga ggatgccagc gaacagctgg aggcgttgga ggcatacttc gaaaattttg
     1081 cgtaaacaga aataaagagc tgacagaact atgttcgaac aacgcgtaaa ttctgacgta
     1141 ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc aaaaacccct gcgtgactcg
     1201 gttaaacagg cactgaagaa ctattttgct caactgaatg gtcaggatgt gaatgacctc
     1261 tatgagctgg tactggctga agtagaacag cccctgttgg acatggtgat gcaatacacc
     1321 cgtggtaacc agacccgtgc tgcgctgatg atgggcatca accgtggtac gctgcgtaaa
     1381 aaattgaaaa aatacggcat gaactaattc aggttagcta aatgcttgat taaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccggt ttggtaatga agctaagacc attattcacg gtgatgccct
     1561 tgccgaactt aaaaagctac ctactgaaag cgtcgatctg atctttgccg acccaccgta
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta
     1741 cattatgaac agtacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttctgga gtacaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa
     1921 cggtgatgct attctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg
     1981 caaaaatcct ccacagccat acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 gcgcgtgcgt tatttaatgg atgaatatga gaaccacccg acgcaaaaac cggaagcctt
     2101 actgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 cggtagcttt actaccggtg ccgtagccat cgccagcgga cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 cgcggaagaa ctggcgaaag tgaaaaaaag aaagacgggc aaccggtcaa aacgatgccg
     2341 ggttagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatgcaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctggca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttacatga cacttaattc attcgtttga tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccagc gcgggtgctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccatcaat aataatcatc
     2821 acaacatcta aatcgaggtt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagag tctgcggatt aaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaagcc gacaatcaat ttttcacgca attgttgaaa cgggtcatgc
     3061 tctaatccag ccgtcaagtg ttcctggatt aactcccgca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgagt cttgttttcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcagcgt cggcaatgtc gttgagtgtc gtcttgctta cgccatgctg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcagag cttcggcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca gagtgagaaa atagcgaagg
     3361 ttaatctatc acctaatgtg tatttatacg agaggctaat
//
LOCUS       DAPHJE010000235         3316 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPHJE010000235 DAPHJE010000000
VERSION     DAPHJE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.238"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            54..836
                     /locus_tag="RH222_004438"
     CDS             54..836
                     /locus_tag="RH222_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV3799347.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(838..1064)
                     /locus_tag="RH222_004439"
                     /pseudo
     CDS             complement(838..1064)
                     /locus_tag="RH222_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(1839..2273)
                     /locus_tag="RH222_004440"
     CDS             complement(1839..2273)
                     /locus_tag="RH222_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV3799348.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDILCCVSCGCPLTLKR
                     GNHGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(2261..2662)
                     /locus_tag="RH222_004441"
     CDS             complement(2261..2662)
                     /locus_tag="RH222_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799349.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            complement(2829..>3316)
                     /locus_tag="RH222_004442"
     CDS             complement(2829..>3316)
                     /locus_tag="RH222_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3799350.1"
                     /translation="ALRVDLRFPDVPAATDAAVISRFINALKARIDAYQKRKHREGKR
                     VHPTTLHYVWAREFGECKGKKHYHLMLLVNRDTWCRAGDYRDPGSLAGMIKQAWCSAL
                     GVDAGCYATLVHFPVRPAVWLERDDETGFQQVLERAGYLAKEHTKARGTGERNFGCSR
                     G"
BASE COUNT          815 a          906 c          776 g          819 t
ORIGIN      
        1 aatgtttact aatgccataa tgttattttt ataacatttt acggagagag ttgatggaaa
       61 cgaagcaaaa agagcgtatc cgacgtttga ttgaaatact taagaaaacc gacagaatcc
      121 atttgaaaga cgcggcacga atgctggaag tttctgtaat gactattcgt cgcgatctcc
      181 atcaggaaga tgaacctctg ccactgaccc tactgggtgg ctatattgta atggtgcata
      241 aacccgcacc atccatgcca gtaatccagg acgttccgag aaatcatcgt gatgacttac
      301 ctattgcaat tctggccgcc ggaatggtta atgaaaatga tctgatcttc tttgataatg
      361 gccaggagat accgctcgtt ataagcatga tcccggatgc aatcaccttc actggcatct
      421 gttactcaca tcgtgtcttt gttgcgttga atgaaaaacc taatgtgaca gcaatacttt
      481 gtggtggtac gtatcgtgcc agaagtgatg ctttttacga tgccagtaac tcttcgccat
      541 tagactctct caatccgcga aaaatattta tttccgccag cggtgtacat gatcactttg
      601 gcgtcagctg gtttaatccc gaagatcttg ccactaagcg taaagcgatg gcccgtggac
      661 taaggaaaat tttgctcgcc cgccacgcct tgttcgatga agtagcctct gccagcctcg
      721 caccgctctc tgcatttgat gttctgatta gcgagcgtcc gttaccggca gattatgtta
      781 cgcactgccg gaatgcttct gtaaagatca ttacacctga ttcagaagac gaatgactta
      841 ctgaaaaaac accgtactct tgttaaacat cgtcggattg gactgattac gttgcacttt
      901 catcacatat tccagtttat caatttggct tatcatctac tccagacgct ggtcatcatt
      961 gaccaatagt ctgatatggc ttttgtcgct gtcctgaatc ggcaggcaga aaatgccttc
     1021 aacgttaaaa gcacgacggg aaaaagccca caaaggttag tcattacgcc cggataataa
     1081 acaggctcct ccctgttatg gaagccgttg tattgtccat aatgtctggg tttttaatgc
     1141 acactggata acaaccggac gaatcttcaa atattactgg cgctgacagc cggtttgaat
     1201 tcatctcaca accctgcata gagcgaatct cctgcctgca cgtcactcca ctccatggta
     1261 tcaacttcac actctttatc tgcggctagt ttcagtcacc agataagcat cgactatgaa
     1321 aggtgagcca tgacaacata acgttggtaa cgctctgacg ccttaacgga agatgcctgc
     1381 caccacaggg aatgtaaacg actgaagtgt ggcctttaat gccgtgaacg gctcatggtc
     1441 tcctggcacg gttgccgccc caacctgtaa caacattcca cagtacaatg tctgtcagag
     1501 ccggagcctc ccatgcctgt tgaataaaac cccggatgct tcccccgatt ctgcaagtaa
     1561 tacttcggtt atttacacta ttttatccct gttgaatttg gttaagccgg tgcagacgcg
     1621 ccgggaaacg cacgcctgaa ccggcacatt aaaatacaat acaaccccct gttactggag
     1681 atacgaatgg aatgacgagg cagcctgtca gtcacaggag ggctgttggc tcaaaatcaa
     1741 cggaaaggcc accaataccg gaattaagct ccactgtccc cgcactggca atccattccc
     1801 gggaacggca tcaccgcaaa ctgcgccgga ggcaaccatt acagcctctg taacggatta
     1861 tcagccctgg catcttccga cggcacctcc tgccgccccc acgccctgct gtataccccg
     1921 gtaccacaac gcgggcagca cttttctcct tcataatctt cgtcacacat cacacaaaac
     1981 cactttctca ccttcaccgt ataatccgga accgtcatac cctgcagatg caaacgacgg
     2041 gcctctttct ctgccgggtc aagccaggtg catcgcagca ggattttctc agcaacagtc
     2101 atctggtcat gttcaaacca cggtggttgt ccgtgattgc cccgcttaag tgttaaaggg
     2161 catccacagg acacacagca caaaatgtcc tgtcggttgt gcttcgcagc ttctttcgcc
     2221 gtaacaaaat ggcctttgct gtcattcgcc atataacagt tcagaattcg catatttcct
     2281 cctgagttgt ccggggaata ctgaatcctc cggtctggca gtttgtgcag tactgctccc
     2341 cataataatc gtggtgacat tgtctgcagt gccagctggc tttacgcacc acgggtaagg
     2401 catccggtac gaattgctgc agacgcttaa tcaagcgtac ctccctgcgt tccggtctga
     2461 cataaggaca ctgctgaccg tgcgctgtca gcccgtcgtc agtgtgttca aaccagggac
     2521 gttcagtgtc gtattgcggg tggtatctga gtgcactgcc gcaaaggtgg caagtgtagc
     2581 gatcgtaagg tgcagtctgt gcggtacggg caccagtcag acgcccgttg ccatcaagtg
     2641 cgagaaagga ttttgcgtac atcgtcatat tcctcagccg ccagacgaca cgcaggcaac
     2701 agccgtcccg gtacgggcag cgtggtcagg tgtgaatagc ggttttcagt tcagtctcag
     2761 aagggaaaat cagtgtggtc gctgtgaacg cgtcatcaca ctaaggtcag ataagtcgtc
     2821 tgtcgggctc agccacgact gcagccaaag ttgcgctcac cggtgccgcg agctttggta
     2881 tgctccttcg ccagatagcc agcacgttcc agcacctgct gaaagccggt ttcatcatcg
     2941 cgttccagcc acaccgccgg cctgaccgga aaatgcacca gcgtggcata acatccggca
     3001 tccaccccca gggcactgca ccaggcctgt ttaatcattc cggccagtga ccccggatcg
     3061 cggtaatcgc cggcacgaca ccaggtatcc cggttgacca gcagcatcag atgatagtgt
     3121 tttttacctt tgcactcccc aaactcccgg gcccagacgt aatgcagggt ggtgggatgc
     3181 acgcgtttac cttcccgatg cttacgtttc tggtaagcgt cgattcgggc tttcagggca
     3241 ttgatgaagc gggatatcac agctgcgtcc gttgctgccg gtacatccgg gaaacgcagg
     3301 tcaacccgca atgccg
//
LOCUS       DAPHJE010000236         3260 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPHJE010000236 DAPHJE010000000
VERSION     DAPHJE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.239"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(46..2892)
                     /gene="ag43"
                     /locus_tag="RH222_004443"
     CDS             complement(46..2892)
                     /gene="ag43"
                     /locus_tag="RH222_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3799351.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRTGVAIALSLAA
                     VTSVPALAADTVVQAGETVSGGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINK
                     NGRQIVAVEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGSALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQASNTTVGHRGTLMLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIYFDNQTTPDAVLSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVHGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          665 a          972 c          847 g          776 t
ORIGIN      
        1 gccccggtca tgatgaccgg gacctgaagg agagtgacct gattatcaaa aagtcacatt
       61 cagcgtggcc tgaccgttat aaccttcagc gctgctgccg ctgacgctgt gggcataacc
      121 ggcctgaacg cccagggtga tattttcccg gacacgggct tccagtccgg cctgcaggtc
      181 cagtgacgtg ccattccggg acggtgagaa cgtcatgtta ctgccggcgg cggctgtacc
      241 catgctcatg tcaccccggg agctgaaagt gcggataaca gaaggctgta cccaccagtt
      301 caccggcagt tcactcacac ggtgttttgt actgtcgcgc agagtgtcac gggatgaggt
      361 gccttcgcca aaggtcatat cgttgtggct gcccagacgg aagccggcac gcacatgttg
      421 tgcactgcca tgcccgaact tcacataacc ggcgttatcc tggccgtcat ccagggaaag
      481 tccctgccag gtatactgca gttgtggctc cagcatcagg ttgtcagtga tactgaaggg
      541 cagaccggtt tccagtgagc ccagccagcc ccagccgcgg gcgcggaagt cgttattgtc
      601 cgatgacgct ttcatgctgt ggcgggttcc ctgtgccaca atgtcagccc acaggccgga
      661 ggacgtgtgt gtcagattca ggtatccgcc caggctgccg gcatcatccc ggaccgtgcc
      721 ggcgcgggaa ccgtcatcat ccttaacatc aacggaagaa tggcctgcgg caccatgcac
      781 ccctgtcgtc agagacatac cggcaacctc tgttctgagc aggtcaccct ccagacggac
      841 gaagccatag ctgccgctgc tttccggcgt ggctccacgg gcaataccgc cgttgttatc
      901 gtgaccgaga tgaccgccct gaatgctgag acggacgctg ttattttcac cgtttacacc
      961 ggtctgatgg ctgcgggagc ctgccagaat ccggtcatag tccattgcct gtgtcagcat
     1021 ggatgtatac agggggactt cagcacgata agcattttca ctgcgcaggt accagtcttc
     1081 atcgctgtca cggttcaggg tgtagttaaa ggcgccggcc tgaagcgggc gactcagggc
     1141 aaacgcacct tcttctgtgg tggcgccatt ctgtgcatcc acaacccgga taccctgtcc
     1201 ggaggttgcc accccgaggt tgctgtttcc gacatttgta aacgcaagcc aggttttgcc
     1261 ggttgcctga ccaccattaa tcaccagctg gtcagaggca ttgctgccat caaggcgaac
     1321 acgcatattg atggtgccac cctgaccggt gaggttactg gtggtcagtt tatggaacgt
     1381 taccggggag tcgccttttg caacagcacg gctcagtacg gcatccggtg ttgtctgatt
     1441 gtcaaagtaa atctcccctg cgttaacaat atcgccggta ctgaccacat caccattcag
     1501 taccatgctg gcgcctttac tgagctgtgt tctgccactc agacttcccc cggcagccag
     1561 catcagtgtt ccacgatgtc cgacagtggt gttgctggcc tgtcccccgg cattaaccgt
     1621 aaagctgccg ccattttcca gcagcagtcc gctggcctga ccggatgtgc catcaatact
     1681 gaacttaccg ctggcattgg tcccctcaac agtggcacca ctgtctgcaa tcagggcact
     1741 accggatgtc atggtgacat ctgttgcctt accaccggca gacactgcca gggttccgcc
     1801 gtcatccacc cgtgttttct gcgctgaatg gccttccagt acatccaggc gaccgccgga
     1861 ctccagaaca acgccgtcag ccttaccgtt ttccaccgtg aaattgccca gacggttgct
     1921 gccaagaacc gttgccgcag tactggtgac cagtgctcca ccctgtttca gggtgacatt
     1981 cgtggctgta ccaccggcgt tcacctgcag tttgcctttc tggttaacgg tggtgaaatc
     2041 cgccagacca ccttccttga caatctgcca gccgtcactg tttacaaccg tgtcagaggc
     2101 tgtgcctccg ttgtgcacat actggtaacc gccattcagt gtggtatcca gcgcatgccc
     2161 gtgtaccgtc tggtcgccgc cggcataaac cacagtggta tttgctgttc cttcaacagc
     2221 cacaatctga cgaccatttt tattgatggt ggtacgtacg gcatttccgc gaacaaactg
     2281 cccggtatca ccattttctg catctggtcc cccttccgcg ccggtattca caaccgtgtc
     2341 ggttgccatt gcgccggatt tgacggcctg ccagcccttc tcattaatga cggtacccgt
     2401 tgcaatcccg ccttcatgta cccactgctc accgccgttc agagtggtat tcactgcctg
     2461 cccctgaagg ctctgtccgc ctccggcact gataaccgtg tctgaaacgc ttcctccggc
     2521 attcactctc tgaagaccac caccggtgac agtagtgttg ttggcgatac cgccattttg
     2581 tatccattgc ccgccggtat tggcctcgtt atccggccca tactccagac cggtactgat
     2641 ggtcattccg ttggccgtac cgagaacaat ctggttgtca tgatttgtca gtgttccgcc
     2701 gctcacggtt tctcccgcct gtacaaccgt gtcagcagcc agtgccggaa ctgatgtgac
     2761 agcagcaaga gacagcgcaa tcgccacacc ggtgcgtttt ccccgtgagc gcgccagttc
     2821 ggaagccacc accagggtgc ccgtaatgtg attccatacc agcctgtagc tggtgttcag
     2881 atgtcgtttc atcagctttt ccttacagag ggtgaataaa aaagccggta cccacaaaag
     2941 tggataccgg caggtacaaa acagcatggt cagataaagt ggtttttccc ggacataaat
     3001 gcttcccctc tctcatccgc cagagaagta tcagtccgtt gtgtgcaatt ccgttctgca
     3061 tgatgaagag attcagctgc cttttcgcag accattaacc actataaacg atcgatggta
     3121 acgatcgata cctgttttat cgatcgtttt attctattaa cagcagggca gtcacgcaag
     3181 agaaacagga gggggatggg gtgtttgtat catgttttca gttggttaac gcaatgtaat
     3241 caggcatttc ccctggcaga
//
LOCUS       DAPHJE010000237         3088 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPHJE010000237 DAPHJE010000000
VERSION     DAPHJE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.240"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            56..1738
                     /locus_tag="RH222_004444"
     CDS             56..1738
                     /locus_tag="RH222_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV3799352.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1790..2947
                     /locus_tag="RH222_004445"
     CDS             1790..2947
                     /locus_tag="RH222_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV3799353.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLAGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2913..3035)
                     /locus_tag="RH222_004446"
     CDS             complement(2913..3035)
                     /locus_tag="RH222_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799354.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
BASE COUNT          943 a          667 c          629 g          849 t
ORIGIN      
        1 tatttctctt cgtaaaatta cttcggaatt aagtacaata agaagaggaa catttatgaa
       61 gtctgcatta aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg catctggtat
      121 ggctgcattt gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt
      181 tgctttctca gactttacgc cgactgaata cagtaccaaa ggaaagccaa atattatcgt
      241 actaaccatg gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc
      301 aaaaacaatg gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat
      361 tgaagctgca caaaaatcaa cgccgacgct cctttcatta atggatgaag gcgtacggtt
      421 tactaacggc tatgtggcac acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg
      481 tcgagctccc gcccgctttg gtgtctattc caataccgat gctcaggatg gtattccgct
      541 aacagaaact ttcttgcctg aattattcca gaatcatggt tattacactg cagcagtagg
      601 taaatggcac ttgtcaaaaa tcagtaatgt gccggtaccg gaagataaac aaacgcgtga
      661 ctatcatgac aacttcacca cattttctgc ggaagaatgg caaccgcaaa accgtggctt
      721 tgattacttt atgggattcc acgctgcagg aacggcatat tacaactccc cttcactgtt
      781 caaaaatcgt gaacgtgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc
      841 aattggcgtt gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta
      901 taatgctccg cacctgccaa atgataatcc tgcaccggat caatatcaga agcaatttaa
      961 taccggtagt caaacagcag ataactacta cgcttccgtt tattctgttg atcagggtgt
     1021 aaaacgcatt ctcgaacaac tgaagaaaaa cggacagtat gacaatacaa ttattctctt
     1081 tacctccgat aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg
     1141 ctataagagt cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg
     1201 aaaacttcaa cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc
     1261 tcttgatgca gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct
     1321 gccctggttg caagataaga aacaaggcga gccacataaa aatctgacct ggataacctc
     1381 ttattctcac tggtttgacg aggaaaatat tccattctgg gataattacc acaaatttgt
     1441 tcgccatcag tcagacgatt acccgcataa ccccaacact gaggacttaa gccaattctc
     1501 ttatacggtg agaaataacg attattcgct tgtctataca gtagaaaaca atcagttagg
     1561 tctctacaaa ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt
     1621 cgttaaagag atgcaaggcg tggtaagaga gtttatcgac agcagccagc caccgcttag
     1681 cgaggtaaat caggagaagt ttaacaatat caagaaagca ctaagcgaag cgaaataact
     1741 aaaccttcat gcggcggatt tttccgccgc cttattgagc gagatagcga tgcacgttac
     1801 agccaagccc tccagttttc aatgtaatct caaatgtgat tactgttttt accttgaaaa
     1861 agagtcgcag tttactcatg aaaaatggat ggatgacagc actctgaaag agttcatcaa
     1921 acaatatatc gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg gtgaacccac
     1981 tctggctggc ctggattttt tccgtaaagt tattcactat caacaacgct atgcaggcca
     2041 aaaacgtatt tttaatgcat tacaaacgaa tggcatttta ttgaataatg aatggtgttc
     2101 ctttctcaaa gaacatgaat ttctggctgg tatctcgatc gatggccccc aggagttaca
     2161 tgaccgttac agacgcagta attcaggtaa cggtactttt gcaaaagtga tagcagccat
     2221 cgagcgtctg aaatcatatc aagtagagtt taatacgtta accgtcatta ataacgttaa
     2281 tgtccattac cctcttgagg tttatcattt tttaaaatct atcggcagta aacatatgca
     2341 atttatcgaa ttgctagaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa
     2401 cacattccgt atcattgatt tttctgtgcc tcccacggct tatggcaagt ttatgtcaac
     2461 catttttatg caatgggtta aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag
     2521 ctttgtcagc cgttttttgg ggaatgggca tacaagttgt attttccaag agtcctgcaa
     2581 ggataatctg gttgttgaaa gtaatggaga catttacgaa tgcgaccatt ttgtctatcc
     2641 acagtacaaa attggaaaca ttaataaatc tgaactcaaa acgatgaaca gtgtacaact
     2701 gacagcgcaa aaaaaacgga tttcagcgaa atgtcagcaa tgtgcatata aacctatctg
     2761 caatggcggt tgtcctaagc atcgtattac taaagtaaac aatgagactg tttcttattt
     2821 ttgcgaaggt tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggtaga
     2881 gttagctaag aacagagtac cgctttatca cattatggat gttgcaaaac aaatggagaa
     2941 taattaataa cacgccctct tagctccagt cttagcctcc gtcttatgac cttaacaaag
     3001 cacccgacat aaattcctgt cgggtgtgtc gtcatcatca tcttcccttg cgaaaatcct
     3061 ggcatacgtg gtatgcgtca gggcatta
//
LOCUS       DAPHJE010000238         3002 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPHJE010000238 DAPHJE010000000
VERSION     DAPHJE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.241"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(189..265)
                     /locus_tag="RH222_004447"
     tRNA            complement(189..265)
                     /locus_tag="RH222_004447"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Asp,seq:gtc)
     gene            complement(398..1129)
                     /gene="dnaQ"
                     /locus_tag="RH222_004448"
     CDS             complement(398..1129)
                     /gene="dnaQ"
                     /locus_tag="RH222_004448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV3799355.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            1194..1661
                     /gene="rnhA"
                     /locus_tag="RH222_004449"
     CDS             1194..1661
                     /gene="rnhA"
                     /locus_tag="RH222_004449"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV3799356.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1658..2380)
                     /locus_tag="RH222_004450"
     CDS             complement(1658..2380)
                     /locus_tag="RH222_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV3799357.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2414..>3002
                     /gene="gloB"
                     /locus_tag="RH222_004451"
     CDS             2414..>3002
                     /gene="gloB"
                     /locus_tag="RH222_004451"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV3799358.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELR"
BASE COUNT          810 a          720 c          713 g          759 t
ORIGIN      
        1 ctggaaatat atttaagctg aatggttcga tatcattatt gaattttaat gaatgagaca
       61 acaagcgaga atcacgcact tacgccagag taaaatcatt gaacagagaa atggtggaat
      121 tcaaatacaa aaaaaccgca aaattaaaaa tcttgcggct ctctgaactc attttcatga
      181 gtgaatagtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
      241 ttctaaccga ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg cgaatattac
      301 ggattgcctc gaccttcgtc aacggttttt ctcatctttt gaatcgtttg ctgcaaaaat
      361 cgcccaagtc gctattttta gcgcctttta caggtattta tgcccgccag aggcagcttc
      421 cgcccttctt ctgcaccaga tcaagacggg cttcatgtgc tgcaagctcg tcatctgtcg
      481 caaaaacaac gcgtaactta cttgcctgac gtacaatgcg ctgaattgtt gcttcacctt
      541 gttgctgttg tgtctctcct tccatcgcaa aagccatcga cgtttgacca ccggtcatcg
      601 ccagataaac ttccgcaagg atctgggcat cgagtaatgc cccgtgtagc gttcgtttac
      661 tgttatctat ttcgtagcgg gcacataacg catcgaggct gttgcgctta ccaggaaaca
      721 ttttcctcgc caccgcaagg ctatcggtga ccttacagaa ggtattggtc ttcggaatat
      781 cgcgcttaag caacgaaaac tcgtagtcca taaagccgat atcgaacgct gcgttatgga
      841 tcaccaactc cgcgccgcgg atatagtcca tgaactcatc ggctacttcg gcaaacgtgg
      901 gcttatcgag caaaaattca tcggcaatac catgtacgcc aaaggcttca ggatccacca
      961 gccgatcggg tttgagataa acatggaagt tattgcccgt caggcgacgg ttcaccactt
     1021 caacggcacc aatctcaatg atcttgtggc cttcatagtg cgcaccaatc tggttcatac
     1081 cggtggtttc ggtatcgaga acgatctggc gtgtaattgc agtgctcata gcggtcattt
     1141 atgtcagact tgtcgtttta cagttcgatt caattacagg aagtctacca gagatgctta
     1201 aacaggtaga aattttcacc gatggttcgt gtctgggcaa tcccggacct gggggttatg
     1261 gcgctatttt acgctatcgc ggacgcgaga aaacctttag cgctggctac acccgcacca
     1321 ccaacaaccg tatggagttg atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt
     1381 gcgaagtcat tttgagtacc gacagccagt atgtccgcca gggtatcacc cagtggatcc
     1441 ataactggaa aaaacgtggc tggaaaaccg cagacaaaaa accagtaaaa aatgtcgatc
     1501 tctggcaacg tcttgatgct gcattggggc agcatcaaat caaatgggaa tgggttaaag
     1561 gccatgccgg acacccggaa aacgaacgct gtgatgaact ggctcgtgcc gcggcgatga
     1621 atcccacact ggaagataca ggctaccaag ttgaagttta agcctgtggt ttacgacatt
     1681 gccgggtggc tccaaccgcc tggcgaattc gtggcttgtt tttactctgt ttcatcggat
     1741 ttagcgttaa aggaatagtc cgtttccggg caacaataag ttgtaagcaa ccaagcgcag
     1801 gaatatgcgc attcaatagt tttcctccgt gtttgttcca cgggagaacg tggaaacggc
     1861 tggcgtgtag cacttcaaaa ttcaacaaag agagccagtc cagctgccgc atcagagtaa
     1921 acatccggct gttatagggc gaggttttgc gcaataccgg cacaagtttg cgtaatccca
     1981 ttaaactgat gggattgaag ccactaatga ccagccagcc atcatcaatc aatacccgat
     2041 cggcttcacg caataaacga tgcggatcgg tgcaccacgg caatgtatgt gccagtagac
     2101 aaacatcaac ggatttatcg gcaaaaggaa gatgaagtgg gtccgcctgt acctggacgg
     2161 gcattccttg cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg caattgattt
     2221 ctgcgcttaa attgccaatc ttaagcagat gaaaaccata cattttagtg aaccacgggt
     2281 tgagctggcg ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg ccccagcaat
     2341 caggagccac gacagtttga gggactcttg ccggtttcat cacaaccttc cgtttcacac
     2401 tgagaggtaa tctatgaatc ttaacagtat tcccgccttt gatgacaatt acatctgggt
     2461 tttgaatgat gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag agccagtatt
     2521 aaacgccatt gccgccaata actggcaacc agaggccata tttctcaccc accatcacca
     2581 cgatcacgtt ggcggcgtaa aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg
     2641 tccacaagag acacaagata agggaacaac acaggtagtc aaagatggcg aaactgcctt
     2701 cgttttaggg catgaattta gtgtaattgc cacaccgggt cacactttag gacatatctg
     2761 ttacttcagc aaaccttatc tattttgcgg cgacacgctg ttttctggtg ggtgtggtcg
     2821 gttgtttgaa gggacaccat cacaaatgta tcaatcactt aaaaagttaa gtgcgttacc
     2881 tgacgataca ttggtatgtt gtgctcatga atatacgtta tcaaatatga agtttgcttt
     2941 gagtattctt ccgcacgatt tgtccataaa tgattattat cgtaaagtta aggagttacg
     3001 gg
//
LOCUS       DAPHJE010000239         2955 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPHJE010000239 DAPHJE010000000
VERSION     DAPHJE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.242"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            312..523
                     /gene="hha"
                     /locus_tag="RH222_004452"
                     /pseudo
     CDS             312..523
                     /gene="hha"
                     /locus_tag="RH222_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
     gene            1267..1836
                     /locus_tag="RH222_004453"
     CDS             1267..1836
                     /locus_tag="RH222_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3799359.1"
                     /translation="MTHEYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARINAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPRSLAGMIKQAWCSALGVDAGPYATLAHFPDTSAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHATGERNFGCSRS"
     gene            2002..2385
                     /locus_tag="RH222_004454"
     CDS             2002..2385
                     /locus_tag="RH222_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799360.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREVRLIQRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEVVAG"
     gene            2382..2570
                     /locus_tag="RH222_004455"
                     /pseudo
     CDS             2382..2570
                     /locus_tag="RH222_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013305.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          722 a          766 c          799 g          668 t
ORIGIN      
        1 gaaataaaca ctgtgagtgc gatcagtggg catgagaacg cagtagcagc agattgcgtt
       61 gtacccacag ggctatctct gatagctggc gtataacctg cgtatataaa acgtacctgc
      121 aacccacgtg aacagagtgc attaagcatc cgtctgagcg gtaagcgtat ccgacctggt
      181 gagtgacagt ctgagagacc ggttaagtgg tggcccggga gtaatgagtt tggggagggt
      241 accagaatgc cagtctggtg cggtatgctg acagtatcac gttaattgta gcataaagtg
      301 atgagaggct aatggaagtt aaaaccaaag aggactggct gtatcagttc cgtcgttgtt
      361 catcccggga gacactggaa aagtgatttc ccacacgcgt tataaactta cccctgcgga
      421 gctggaaacc ttcaactctg cggtcgatca ccgactggca gaactgacaa tgaacaagct
      481 ttacgatcgt gttcccgctt cagtctggaa atatgtcatc tgagcgttcg gtaatcacaa
      541 cggcatcgcg ccaggtgtta tccgctttgt gattcttatt ggctcttctc aggttattaa
      601 aagctgaata tgttcctgtt gtcgggaacc aggataatgt cgggaacgtt aagctgtata
      661 acgacaacaa agaattcgaa atcaaaaggg aatggtgtcc ggaggtgtgg gtgatcatgg
      721 gctgattttc gggtattaac ggcctgtatc tgctgaggtt ttctcgtatt taccggggat
      781 ccggtgcgtt actgtctggc acagcaaacg cagaggcggc ggggcgttct gccccgttga
      841 ttccggataa tccctcccct gtaacctact gcaacagtac aaatacactg acgtctgaaa
      901 cgacagccag aatcagcaca ggcagtaacg tcagtagaaa gagcagacga tcggtatttc
      961 ggtaatagcc tgaggaagct gttgacgcgc agctcgggca gcattccgta ttatgagtaa
     1021 ttgcgttact acaatccctg cacaccgttt tgcgtatcac cataacgccc tcctcacagt
     1081 tttatccgta accggttttt tgcattaaaa aaacaacgct gaaaattcaa ccgtcagtcc
     1141 ggagacgaca gtcggggtat cactgagtgc ctgagacatt gcccggttgg agatcacttc
     1201 agggactctg cacatttttt acagcgtggt tattccgtat gaacgaaacc atacggagat
     1261 ttaaccatga cacacgagta caaccccttc tggcaacaac gtatccgtga gacggtgctg
     1321 cgcgcactgg atgttcatcc ccgcctgacg gcattgcggg ttgacctgcg tctcccggat
     1381 gtaccggcag caacggacgc agctgtgata tcccgcttca tcaatgccct gaaagcccga
     1441 atcaacgctt accagaagcg taagcgccgt gaaggtaaac gcgtacatcc cacgaccctg
     1501 cattacgcct gggcccggga attcggggag cttaaaggca aaaaacacta tcacctcctg
     1561 ctgctggtca accgggatac ctggtgtcgt gccggcgatt accgcgctcc gagatcactg
     1621 gccgggatga ttaaacaggc ctggtgcagt gccctggggg tggatgccgg gccttatgcc
     1681 acgttggcgc attttcctga cacgtcggcg gtgtggctgg aacgcgatga tgacactggc
     1741 tttcagcagg tgctggaacg tgctgactat ctggcgaagg aacataccaa agctcacgcc
     1801 actggtgagc gcaactttgg ctgcagtcgt agctgagccc gacagacgag ttatctgacc
     1861 tcagtgtgat gacgcgctca cggccaccac actgattttc ccttctgaga ccggactgaa
     1921 accgccattc acaccagacc aggctgcccg taccgggacg gctgttgcct gcgtgtcgtc
     1981 tggcgctaag gaacatatac tatgtacgca aaatcttttc tcgcacttga tggcaacgga
     2041 cgtctgaccg gtgcccgtac cgcacagact gcaccttatg atcgctacac ctgccacctt
     2101 tgcggcagtg cactcagata ccacccgcaa tacgacactg aacgtccctg gtttgaacac
     2161 actgacgacg ggctgacagc gcacggtcag cagtgtcctt atgtcagacc ggaacgcagg
     2221 gaggtacgct tgattcagcg tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt
     2281 aaagccagct ggcactgcag acaatgtcac cacgattatt atggggagcg gtactgcaca
     2341 cactgccaga ccgggcgctt cagtgaggag gtggtagcag gatgaacggc ataaaaatgg
     2401 ggctggggat cacgccgggt gagcacatta tcagtgccaa ctctgcgctc agcagaaata
     2461 tccggcattg tttctgcctt tcctgtcgtg gccggttgat tttgcagaca gatgctcaag
     2521 gggcgtggtt tgagcatgat ttacatgccc tgagtgcgca acagaaggcg gccttgcagc
     2581 ccctggatta actatgcaga aatcgtcctg aggcactgat aaatacggag aggactctca
     2641 cgtatggttg attgacacca cagtgcgaat tcggcgaatg cgagatcaca gtacgattcc
     2701 gcaccacact accaatcaat accccgggtt ttattcaggt aagcaggact gcggatatcc
     2761 ggtgtcgcgc cgttgtgtca cgaacggggc aggtgcgaaa caccggataa aatgcaggtt
     2821 gcgatacctc tgaacgccct gcgcagagcg gcgattttgg attaagtact cgcgcctccg
     2881 cagtcctcaa acaagtctgg ctggtagctg taaacagacc tcgtacatac cgctctgcaa
     2941 taaatccccg tagca
//
LOCUS       DAPHJE010000240         2909 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPHJE010000240 DAPHJE010000000
VERSION     DAPHJE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.243"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            325..1491
                     /gene="nhaA"
                     /locus_tag="RH222_004456"
     CDS             325..1491
                     /gene="nhaA"
                     /locus_tag="RH222_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV3799361.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1557..2456
                     /gene="nhaR"
                     /locus_tag="RH222_004457"
     CDS             1557..2456
                     /gene="nhaR"
                     /locus_tag="RH222_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV3799362.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2495..>2909)
                     /locus_tag="RH222_004458"
     CDS             complement(2495..>2909)
                     /locus_tag="RH222_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDV3799363.1"
                     /translation="FEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSD
                     VEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPH
                     EGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          695 a          642 c          719 g          853 t
ORIGIN      
        1 tttatctctt catcgcaatt attgacgaca agctggatta tttttgaaat attggcctaa
       61 caaacatcgc cgactgacaa caaattaatt attacttttc ctaattaatc tctcagaaat
      121 cctcacctta agctatgatt atctgggctt agggtcactc gtgagcgctt acagccgtca
      181 aaaactcatc tcaccgctga tggcgcaaat tcttcaatgg atcgtaaaaa acgagttatt
      241 cctacactat aatctgattt taacgatgat ttgtgcgggg taaaatagta aaaacgatct
      301 attcacctga aagagaaata aaaagtgaaa catctgcatc gattctttag cagtgatgcc
      361 tcgggaggca ttattcttat cattgccgct atcctggcga tgattatggc caatagcggc
      421 gcaaccagtg gatggtatca cgactttctg gagacgccgg ttcagctccg ggttggttca
      481 ctcgaaatca acaaaaacat gctgttatgg ataaatgacg cgctgatggc ggtatttttc
      541 ctgttagtcg gtctggaagt taaacgtgaa ctgatgcaag gatcgctagc cagcttacgc
      601 caggccgcat ttccagttat cgccgctatt ggtgggatga ttgttccggc attactctat
      661 ctggccttta actatgccga tccgattacc cgcgaagggt gggcgatccc ggcggctact
      721 gacattgcct ttgcacttgg tgtgctggcg ctgttgggaa gtcgtgttcc gttagcgctg
      781 aagatctttt tgatggctct ggctattatc gacgatcttg gggccatcat tatcatcgca
      841 ttgttctaca ctaatgactt atcgatggcc tctcttggcg tcgcggctgt agcaattgcg
      901 gtactcgcgg tattgaatct gtgtggtgta cgccgcacgg gcgtctatat tctggttggc
      961 gtagtgttgt ggactgcggt gttgaaatcg ggggttcacg caaccctggc gggggtaatt
     1021 gtcggcttct ttattccttt gaaagagaag catgggcgtt ctccagcgaa gcgactggag
     1081 catgtgttac acccgtgggt ggcgtatctg attttgccgc tgtttgcatt tgctaatgct
     1141 ggcgtttcac tgcaaggtgt cacgctggat ggcttgacct ccattctgcc attggggatc
     1201 atcgctggct tgctgattgg caaaccgctg gggattagtc tgttctgctg gttggcgctg
     1261 cgtttgaaac tggcgcatct gcctgaggga acgacttatc agcaaattat ggcggtgggg
     1321 atcctgtgcg gtatcggttt tactatgtct atctttattg ccagcctggc ctttggtagc
     1381 gtagatccag aactgattaa ctgggcgaaa ctcggtatcc tggtcggttc tatctcttcg
     1441 gcggtaattg gatacagctg gttacgcgtt cgtttgcgtc catcagtttg acaggacggt
     1501 ttaccgggga gccataaacg gctccctttt cattgttatc agggagagaa atgagcatgt
     1561 ctcatatcaa ttacaaccac ttgtattact tctggcatgt ctataaagaa ggttccgtgg
     1621 ttggcgcagc ggaggcgctt tatttaactc cacaaaccat taccggacag attcgagcgc
     1681 tggaagagcg cctgcaaggc aaattattta aacgcaaggg acgtggtctc gaacccagcg
     1741 agctgggaga actggtctat cgctatgccg ataaaatgtt caccttaagc caggaaatgc
     1801 tggatattgt gaactatcgc aaagaatcca atttattgtt tgacgttggt gtggctgatg
     1861 cactttccaa acgcctggtc agtagcgtac ttaacgccgc agtggtagaa ggcgagccca
     1921 ttcatcttcg ctgcttcgaa tccacccacg aaatgctgct ggagcaatta agtcagcata
     1981 aactggatat gatcatttct gactgtccga tagactctac gcagcaggaa ggcctgttct
     2041 ccgtgagaat tggcgaatgt ggcgtgagtt tctggtgtac aaatccacca ccagaaaaac
     2101 cgttcccggc ttgtctggaa gaacggcgac ttttgattcc tgggcgacgt tcaatgttag
     2161 ggcgcaaatt gcttaactgg tttaactccc agggattaaa cgtagaaatc ctcggcgagt
     2221 ttgatgatgc cgcgttgatg aaagcttttg gtgcgatgca caatgcaatc ttcgttgccc
     2281 caacgcttta tgcatatgac ttttatgccg ataaaactgt cgtagaaatt ggtcgcgtcg
     2341 agaatgtgat ggaagagtac catgctattt ttgctgagcg gatgattcag cacccggcgg
     2401 tacagcgaat ctgcaatacg gattattctg cgctttttag tccagcggcg cgttaatcgg
     2461 cacctccccc caaagttaag gtgggggaga tagattagtt gtacataacc acgattttga
     2521 ctcggctcat tatttgcccg cttgagacat tatttccata tgtacgcggg cgaataaata
     2581 gaggaatctg attacttcct tcatggggat gctgaaaaga gtagtaattg cttttaccat
     2641 actggatttc attcgtacca tcattggaga gttgaaagcc tatttgaccc gcaccgtcct
     2701 cactgttttc taactcattt tttaatatgc tggttcctga ctcctgtatc gtcactggtt
     2761 caaagtaata ttcaacatca ctgggttcaa aaactaattc accttgctcg ccctggatgc
     2821 agtcaaatct tatatgatga aactgataat tttcattatt gatacgagac agtacaaatc
     2881 gtccagtgtc gatggattga tcttcaaaa
//
LOCUS       DAPHJE010000241         2795 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPHJE010000241 DAPHJE010000000
VERSION     DAPHJE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.244"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..135
                     /locus_tag="RH222_004459"
     CDS             <1..135
                     /locus_tag="RH222_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HDV3799364.1"
                     /translation="RWHGSAEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTLN
                     "
     gene            298..675
                     /locus_tag="RH222_004460"
     CDS             298..675
                     /locus_tag="RH222_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV3799365.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCGYAYLAVYPTPAEPAITV"
     gene            722..1099
                     /locus_tag="RH222_004461"
     CDS             722..1099
                     /locus_tag="RH222_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV3799366.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            1096..1584
                     /locus_tag="RH222_004462"
     CDS             1096..1584
                     /locus_tag="RH222_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799367.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGLPTF
                     DW"
     gene            1604..1801
                     /locus_tag="RH222_004463"
     CDS             1604..1801
                     /locus_tag="RH222_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV3799368.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAWEDIRTLRQLQLPDQNR"
     gene            1886..2731
                     /locus_tag="RH222_004464"
     CDS             1886..2731
                     /locus_tag="RH222_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV3799369.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKTTLLQIDTLIRQ
                     LAEISSMTESIGGKTSLDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRGDRWGLHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENIIIRLDDHIRSVQGKACYEDEMFNIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     MLPSL"
BASE COUNT          709 a          749 c          705 g          632 t
ORIGIN      
        1 cgctggcacg gcagtgctga agcgtatacc ggtcgtgaag tgcaggatat tcccggtgtg
       61 ctggccgtgt ttgcagaacg ccgtaaggac agttttggtc cgtatgtccg gctgatgagc
      121 gtcaccctga actgatgttc cgtcacccgg aaaacgagta cgactggctg agctcagacg
      181 ccgctgtgga tgaggtcctg ctcatcaacg aatacacctt taccgaagcc ggtctgcgcg
      241 ccggctgacc cgtcatcgtt aaactatcat tcaccgattt tctgtgagga ttttatcgtg
      301 tcagacacac tttccgggac aacgcatccc gatgacaatg acgaccatcc ctggtgggga
      361 ctaccctgca cagtgacgcc ctgtttcggc gcccgtctgg tgcaggaggg taaccggttg
      421 cattaccttg ccgaccgtgc cggtatcaga ggccggttca gcgacgcgga tgcataccac
      481 ctggaccagg cctttccgct gctgatgaaa caactggaac tcatgctcac cagcggtgaa
      541 ctgaatcccc gccatcagca taccatcaca ctgtatgcga aggggctgac ctgcgaagcc
      601 gatacccttg gcagttgtgg ttacgcttat ctggctgttt atccgacacc ggcagagccc
      661 gcaatcaccg tataaccccg gatatctccc ctttatcacc acctgttaca gagaacttaa
      721 gatgaataca ttacccgaca ctcacgtacg ggaggcatcg ggctgcccgt ctcccgtcac
      781 catctggcag acactgctca cccgactgct ggaccagcac tatggcctca cgctgaatga
      841 cacaccgttc gctgatgaac gtgtgattga gcagcatatt gaggcaggga tttcactgtg
      901 tgacgcggtg aactttctcg ttgaaaaata cgcgctggta cgtaccgacc agccgggatt
      961 cagcgcagga gccccatcgc agttaatcaa cagcattgat attctccggg ctcgcagggc
     1021 gaccggcctg atgacccgga acaattacag aatggtaaat aacattaccc agggcaagca
     1081 tccggaggca aaacgatgaa actggccctg acgctggaag ccgacagcgt taacgtgcag
     1141 gcactgaaca tggggcgcat tgtcgttgac gtcgatggtg ttaatctcag tgaactgatt
     1201 aacaaggtct ctgaaaacgg ttattcactc cgcgtggtcg ataaatccga ccaacacgca
     1261 accagtacac caccaccgtt aacaaccctt acctgcatac gttgcagcac cgcacatatc
     1321 acggaaacgg acaacgcctg gctgtactcg ctgtcacacc agactaatga cgacggcgaa
     1381 acagaatgga ttcattttac aggcagcggc tatctgttac gtaccgatgc atggtcgtac
     1441 ccggttctgc ggcttaaacg cctgggcctg tccagaacgt tccgtcgtct ggtcgtcact
     1501 ctcacccggc gttatggcgt cagtctcatt catctggacg ccggcgcaga atgtctgccg
     1561 gggctcccca ctttcgactg gtaatcagga caaaagacac tggatgaaat tattaaccac
     1621 agaaacggcg ctggatattc tgattgcgtg gctgcaggac aatattgact gcgaatcagg
     1681 gattatcttc gacaacgatg aggataaaac ggattcggca gcactgctgc cctgtattga
     1741 acaggcctgg gaagatatcc gtaccctgcg ccagttgcag cttccagacc agaaccggtg
     1801 agtctcattc accatacttc attccactga cgccagcctg aacagggctg gcgttttcat
     1861 ttatctgcga aaaggaatat cgattatgtc tgcaatcaca gtctcccgtc cggaagtggt
     1921 caacgggcat acggacgtta tctgctccac ctcagtcagc cacattctgg ctgtgcgaaa
     1981 gactacactg ctgcaaatcg acacacttat ccggcaactg gctgaaatct catcaatgac
     2041 agaaagtatt ggaggtaaaa cttctctgga ctgggccatg aaacaggatt ttcgctgtgg
     2101 ctgctggctc atggagaaac cggaaaccgc aatgaaagcc atcacccgca atcttgaccg
     2161 cgaaatctgg cgtgacctga tgcaacgttc cgggatgctg tcattaatgg atgcgcaggc
     2221 ccgtgataca tggtaccgct cactggagta cgataacttt ccggaaatca gtgaagcgaa
     2281 catttggagc acatttgagc aactgcacca gaataaggat gaagtgtttg agcgaggagt
     2341 gatcaacgtc ttcagagggc tgagctggaa ttacaaaacc aactccccct gtaaatttgg
     2401 cagtaaaatt atcgtcaaca atctggtgag gggggaccgg tggggattgc atcttatcac
     2461 cgggcaacag gcagatcgac tcgccgacct ggaaagaatg ctgtatctgt tcagtggcaa
     2521 accaatccct gacaaccggg aaaatatcat cattcggctg gatgatcaca tccgctccgt
     2581 tcagggcaaa gcgtgctatg aagatgagat gttcaacatc agatacttta agaagggctc
     2641 cgcacacatc acgttcagga aaccagaact ggttgatcgg ctaaatgata ttattgccag
     2701 gcactattcg gaaatgttac cttcattata aagctctaag aatgaattca attatgtatt
     2761 ttagcttcat tccctcacgc aaatttattt ggggg
//
LOCUS       DAPHJE010000242         2792 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPHJE010000242 DAPHJE010000000
VERSION     DAPHJE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.245"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            161..1375
                     /locus_tag="RH222_004465"
     CDS             161..1375
                     /locus_tag="RH222_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HDV3799370.1"
                     /translation="MHKDPSIIVNINLREAKLKKKVREHLQSLGFTRSDSGALQAPGN
                     TKDVIRALHSSQRAERIFANQKFITLRAAKLIKFFASGNEVIPDKISPVLERVKSGTW
                     QGDLFRLAALTWSVPVSSGFGRRLRYLVWDESNGKLIGLIAIGDPVFNLAVRDNLIGW
                     DTHARSSRLVNLMDAYVLGALPPYNALLGGKLIACLLRSRDLYDDFAKVYGDTVGVIS
                     QKKKQARLLAITTTSSMGRSSVYNRLKLDGIQYLKSIGYTGGWGHFHIPDSLFIELRD
                     YLRDMDHAYADHYMFGNGPNWRLRTTKAALNVLGFRDNLMKHGIQREVFISQLAENAT
                     SILQTGKGEPDLTSLLSAKEIAECAMARWMVPRSIRNPEYRLWKARDLFDFISNDSLK
                     FPPSDEIAKTVV"
     gene            1397..>2792
                     /locus_tag="RH222_004466"
     CDS             1397..>2792
                     /locus_tag="RH222_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799371.1"
                     /translation="MNYAIDKFTGTLILAARATKYAQYVCPVCKKGVNLRKGKVIPPY
                     FAHLPGHGTSDCENFVPGNSIIIETIKTISKRYMDLRLLIPVGSNSREWSLELVLPTC
                     NLCRAKITLDVGGRSQTLDMRSMVKSRQIGAELSVKSYRIVSYSGEPDPKFVTEVERE
                     CPGLPSEGAAVFTALGRGASKGFPRAQELRCTETFAFLWRHPVAPDFPDELEIKSLAS
                     KQGWNLALVTIPEVPSVESISWLKSFTYLPVVPARTSITAIWPFLNQKTSINHVECVH
                     SDTILLSANMAPTSSENVGPTMYAQGSSLLLSAVGVEKSPAFFILNPGENDFVGVSGS
                     IEQDVNLFFSFYKKNVSVPRKYPSIDLVFTKRNKEKTIVSLHQRRCIEVMMEARMFGH
                     KLEYMSMPSGVEGVARIQRQTESSVIKLVSNDDIAAHDKSMRLLSPVALSQLSDCLAN
                     LTCHVEIDFLGLGKI"
BASE COUNT          837 a          505 c          612 g          838 t
ORIGIN      
        1 caaaatctat aagctcccag aattgcttgt ataaatgcta tcattggcga tgtcccgatc
       61 gagggagcaa ggaggggact ctcttgtgcc atgcgattaa tcactggggc tctaagtgaa
      121 atttagtggg actaaatact aattggaacg tgagataaaa atgcacaaag atccctctat
      181 aatagttaat atcaaccttc gagaagccaa actgaaaaag aaggtacgtg agcatttaca
      241 atccttgggt tttacaagat ctgattctgg agcgctccag gccccgggaa ataccaaaga
      301 tgtaatacgg gctcttcata gttctcaacg agctgagcgg atatttgcaa accaaaagtt
      361 cataacgcta agagcggcaa agcttattaa atttttcgca tccggcaatg aggtcattcc
      421 ggataagatt tcaccggtac ttgaacgtgt aaagtcagga acctggcaag gagatctctt
      481 taggttagca gcattaactt ggtccgtacc tgtttcaagc ggatttggaa ggcgtctccg
      541 gtatcttgta tgggatgaaa gcaacggaaa attgataggg ctgatcgcaa ttggtgaccc
      601 tgtgttcaac cttgcagtcc gagataattt gattgggtgg gatactcatg ccagaagttc
      661 ccggcttgtt aatttgatgg atgcatacgt cctcggtgct cttccccctt ataatgccct
      721 gctgggagga aaattaattg catgtctgct tcgtagccgc gatctttatg atgactttgc
      781 aaaggtctat ggtgataccg ttggagtaat atctcaaaaa aagaaacaag cacgtctttt
      841 ggctattaca acaacatcgt ctatggggcg ctcatcggta tataaccgtt taaagctgga
      901 tggaattcaa tatttaaaat cgattggata tacaggcggt tgggggcatt ttcatatacc
      961 tgatagcttg ttcattgaat tacgtgatta cttacgtgat atggatcacg cttatgcaga
     1021 tcattatatg tttggtaatg ggcctaactg gcgtttacgt acaactaagg cagctttaaa
     1081 tgtactagga tttagagata atttgatgaa gcatggaatt caacgtgaag tttttatcag
     1141 tcagctagca gaaaatgcaa ctagtattct gcaaacaggc aaaggtgaac cagatctaac
     1201 ctctttgctt tctgctaaag agatagctga gtgtgcgatg gcacgatgga tggttccacg
     1261 atcaattcgc aatccagaat atcggctttg gaaagcaaga gatctatttg attttattag
     1321 taatgactcg ctaaaatttc ccccgtctga cgagatagcg aaaacagttg tctaatctta
     1381 actgaagggg gagtaagtga attacgctat tgataagttc accgggacac tgatattagc
     1441 agctcgagca acgaaatatg ctcaatatgt ttgcccagtt tgtaaaaaag gtgttaacct
     1501 ccgtaaaggg aaggttatac ccccatattt tgctcatttg cccggacatg gtacgtcaga
     1561 ctgtgaaaat tttgttcccg gaaattccat cattatcgaa actattaaaa ctatttcaaa
     1621 gcgatatatg gatttgcgct tattgattcc tgtcggaagt aatagtcgag agtggtcatt
     1681 agaattagtg ttgccaacct gtaatttatg tagagcaaag ataacgttag atgtaggagg
     1741 cagaagccaa acgcttgata tgaggagtat ggtaaagagt cgccagattg gtgctgaatt
     1801 atcagtaaaa tcttaccgta ttgtttcata tagtggtgaa cccgatccaa aatttgtaac
     1861 agaagttgaa agagaatgcc ccggtttacc ttctgaggga gcagcagttt tcactgcttt
     1921 agggcgtggg gcatcgaagg gatttccacg agcacaagag ttaagatgta ctgaaacatt
     1981 tgcctttctt tggcgacacc ctgttgctcc agattttcct gatgaattag aaataaaaag
     2041 tttagctagt aaacagggat ggaatttagc tcttgttaca attcctgaag tcccttctgt
     2101 ggagagtatt tcatggctaa aatcttttac ataccttcct gttgttcctg ccagaacatc
     2161 tattacagca atttggccgt tcctaaatca aaaaacaagt attaatcatg tcgaatgtgt
     2221 tcattctgac acaatattgt tgtcagcaaa tatggcacca acatcatcag aaaatgttgg
     2281 accaactatg tacgcacaag gttcctcttt attactttca gcggttggtg ttgaaaaatc
     2341 acctgctttc ttcattctta atcctggaga aaatgacttt gtgggcgttt ctggctcaat
     2401 tgagcaggac gtaaacttat ttttttcttt ctataaaaaa aacgtttctg tacccagaaa
     2461 atatccctca atagatttgg tttttactaa gaggaataaa gaaaagacca tcgtttcctt
     2521 acatcaaaga agatgcattg aagttatgat ggaagcacga atgtttggcc ataaattaga
     2581 atacatgtct atgccttctg gtgttgaagg agtggcaaga attcaaagac aaactgaaag
     2641 tagtgttatt aagttagttt ctaatgatga cattgcagct catgataaga gcatgcggtt
     2701 actatctcct gttgcgttat ctcaattatc tgattgctta gcaaacttaa catgtcatgt
     2761 agaaatagat tttttaggtc ttggtaaaat at
//
LOCUS       DAPHJE010000243         2777 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPHJE010000243 DAPHJE010000000
VERSION     DAPHJE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.246"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..853
                     /locus_tag="RH222_004467"
     CDS             95..853
                     /locus_tag="RH222_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV3799372.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(906..1013)
                     /locus_tag="RH222_004468"
     CDS             complement(906..1013)
                     /locus_tag="RH222_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799373.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1189..>2777)
                     /gene="espL1"
                     /locus_tag="RH222_004469"
     CDS             complement(1189..>2777)
                     /gene="espL1"
                     /locus_tag="RH222_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDV3799374.1"
                     /translation="LLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKT
                     IQPTVNLNGSAFFLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHV
                     PLVRDAEFRALLRHPPAGSRVIASKDFGFALDIFFGRMMANNVSHMSAILYIDNHTLS
                     VRLRIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADR
                     YVRNCAIAILPLLPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQL
                     HILPEKEIIEIITAQNSVGTPALFLAMMNGHTDNVKIFIQEIQSLVDIHIIHEDNLVK
                     LLQTKSANETPGLYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKTHDGE
                     PGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKG
                     NEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYA
                     AINAISQSLSFSADELKTYL"
BASE COUNT          750 a          575 c          625 g          827 t
ORIGIN      
        1 attcgttacc tttccaggga cgctaatctt tgatccttaa gaaagtttcc gggaccgcga
       61 tggtctcttt ttttgttgta cggactcttg ataaatgaag cttttgaaga cagttcctgc
      121 catcgttatg ttggcggggg gcatgtttgc gtcactgaat gcagctgccg atgattccgt
      181 ttttactgtc atggacgatc ctgccagcgc taaaaaacct ttcgaaggta atctgaacgc
      241 aggatatctt gcacaatcag gcaatacgaa aagctcctca cttacggctg acaccaccat
      301 gacctggtat ggacaaacca ctgcctggtc gctgtggggg aacgccagta atacttcttc
      361 taatgatgag cgttcttcag aaaaatatgc ggcgggcggg cgtagccgtt ttaatttaac
      421 tgattatgac tatttatttg gtcaggcaag ctggctgaca gaccgttata acggctatcg
      481 agagcgcgat gtgttgaccg cgggttatgg tcgtcagttt ttgaatggcc cggtacacag
      541 tttccgcttt gaattcggtc caggtgtgcg ttacgacaaa tataccgaca atgccagtga
      601 aacccagccg ctgggctatg cctccggggc ttatgcatgg cagttgactg acaacgctaa
      661 atttacgcag ggtgtttcgg tctttggcgc ggaagacaca acgctgaact ccgaaagcgc
      721 attaaatgtt gctattaatg aacattttgg actgaaggtg gcttacaacg tcacatggaa
      781 ctctgaacct ccagagtctg caccggaaca taccgaccgt cggacaacct tatcgctggg
      841 ctacagcatg taaaattatc tgccggaccg tgttatgcgg tctggctgtc ttcgtttgtc
      901 actgctcagc cccaaatcat tgctgcccaa cgtagcaaca acatagcgcc acagattgcc
      961 agtagcacca ccaccatctt ccagtgtttt ttatcaaagc gtttagtcgg cataccagtg
     1021 tcctgcatat ctcttgtgcg tgataacgga gtgtaacaaa agggacaggc atcaagggat
     1081 tttttgttat tacagctgtc gggtggcgtt gtgcacatgg tcactgtttc tgcgttttat
     1141 gaaaaaaata aaattatgta tcgatataca aatatcataa atagctggtt ataaatacgt
     1201 ctttagttca tcagcactaa aactcaggct ttggctgatt gcattaatag cagcataata
     1261 tgtttctaca gttttataat gattatgatg aatggctata tgaacagctg acatgttgtc
     1321 atgattttta gcagccaaca gtgtaaataa ctgatgttga ctaaaagaat gtttttttgc
     1381 aaaagcaccc agcgtcgata tataagataa cacgacgtct tcattaccct tgctcatggc
     1441 gatgtataat gcaggggttc cctgtgctgt agctcctttt aataaatcca tgatgttagc
     1501 tttgctcaac ttgtatttaa aggcgatgcc attaatttta gagaggaacc gtgtgacaca
     1561 caaagggtga ttattttcca ttgcggcgta taatcctggc tcaccatcat gtgttttcat
     1621 ggctaagata ctcatcacca gttttttgtt taaaagctct tgtgctattg gagtggttaa
     1681 tgcattcaga aagatatcga ttatctcatc gaatccatac aacatggaga tataaagtcc
     1741 aggtgtttcg ttagcacttt tagtttgcag taatttaacc agattatctt catgaatgat
     1801 gtgaatatct accagtgact gaatttcttg tataaatatt ttcacgttgt cagtatgtcc
     1861 gttcatcata gccaaaaata aagcaggtgt accaacgcta ttctgcgcag ttattatttc
     1921 aatgatttct ttttcaggga gtatgtgcaa ctgtctgaat aatgtaataa gctgttgagt
     1981 ctggcctgtg gccattatta acaacattgc cgatggatgt ataggggcaa atggcattcg
     2041 tgtcgcgata cccgtgaaaa tagcccctgg aactccctca ggtaataggg gcagaatagc
     2101 aattgcacag ttgcgaacat acctatcagc ccaggtctga gcatcgggac ctgaactgat
     2161 gaacttatca agcgagagaa agccccgtgc tgtcctgtgg gtgcctctga cggcaacgtt
     2221 ggtatcgttc gggtcgtaaa cggacacaac atagtttaat tgcccatacg ccgactgctt
     2281 tattcgtagc cttactgaca aagtatgatt gtctatatat aaaatcgcgg acatatgact
     2341 gacattgttt gccatcattc gaccgaagaa aatatctaaa gcgaagccaa aatccttact
     2401 cgcgataacg cgactccctg caggaggatg gcgaagtaat gctctgaact ccgcgtcccg
     2461 gactaagggg acatgctgct gtatggattc aacggaggag taatgtttaa tcagattgac
     2521 tttgcctttt ccatttctca gtgaatcaag ggcgtattgc aatgacagat ggcgacaaaa
     2581 aatagcgtca gaaggagatt gcaaaaaaaa tgcactacca ttgagattta ctgttggttg
     2641 tatagttttt tctctatgga tttttttaga cagtttcata caatggttac gattttcggc
     2701 agttaactgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
     2761 cgtggtttct agtaata
//
LOCUS       DAPHJE010000244         2750 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPHJE010000244 DAPHJE010000000
VERSION     DAPHJE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.247"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            54..2333
                     /gene="ydeP"
                     /locus_tag="RH222_004470"
     CDS             54..2333
                     /gene="ydeP"
                     /locus_tag="RH222_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV3799375.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGMLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2581..>2750
                     /gene="safA"
                     /locus_tag="RH222_004471"
     CDS             2581..>2750
                     /gene="safA"
                     /locus_tag="RH222_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDV3799376.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLT"
BASE COUNT          733 a          641 c          673 g          703 t
ORIGIN      
        1 tgcatcatcg ctattacaaa tcctaataat tcatttccac acaggataag tagatgaaga
       61 aaaaaattga atcctaccag ggtgctgccg gtggttgggg tgctgttaaa tccgtagcga
      121 atgcagtacg taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata
      181 agccagaggg ctttgactgt ccgggttgtg catggccaga tcctaagcac agtgcgtcat
      241 tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa
      301 atgcctcttt ctttgctgag aatacggttc aatcattact tacctgggga gatcacgagc
      361 ttgaggctgc gggacgtctc actcagcctt tgaaatatga tgccgtcagc gactgttaca
      421 agccattaag ctggcaacaa gctttcgacg aaattggcgc acgccttcaa agctatagtg
      481 atcccaatca ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt
      541 atcagctttt tgcccgtgaa tacgggagca ataactttcc cgactgctcc aacatgtgcc
      601 atgaaccgac aagcgtgggt ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc
      661 tggaagactt tgagaagtgc gatttagtca tttgcattgg gcataaccct ggtacaaacc
      721 accctcgcat gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca
      781 tcaatcctct acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa
      841 tgctgacgaa ctctgagact cagttggcca gtgcctacta taacgtgcgc attggtggcg
      901 atatggcgtt gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg
      961 ccgcaggtcg gccttcattg cttgatgacg agtttattca aacgcatacc gtcggctttg
     1021 acgagctacg ccgtgacgtt ctcaattccg agtggaaaga tatcgaacgt atttctggac
     1081 taagtcagac acaaatcgcc gaactggctg acgcatatgc cgctgccgaa cgcaccatta
     1141 tctgttacgg aatggggatc actcagcacg aacatggtac ccagaacgta cagcaactgg
     1201 tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac
     1261 gtggacactc taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag
     1321 agtttctggc tcgtctgggt gagcgctatg gcttcacccc acctcatgca cctggacatg
     1381 ctgcaattgc cagcatgcaa gcaatatgta cggggcaggc tcgagcattg atctgcatgg
     1441 ggggcaattt tgcgctggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat
     1501 tagatttggc ggtacacgta gccactaagc ttaaccgctc tcatctgttg accgcacggc
     1561 atagctatat tctgccggtt ctgggacgta gcgagattga catgcaaaaa aacggtgcgc
     1621 aggcggtaac cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc atgttaaaac
     1681 ccgccggtgt aatgctgaaa tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac
     1741 taccccagag cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg
     1801 acattgaagc tgtgctgcca gagttcgccg actataacca acgcatccgt catcccggtg
     1861 gttttcacct gataaatgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta
     1921 atttcattac cagcaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctgg
     1981 tcatggcgac agtacgcagt cacgatcaat acaacacgac gatttatggt atggatgatc
     2041 gctatcgagg ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa
     2101 tttgccgtgt aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc
     2161 gcagctcacg ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac
     2221 tggtgaccta ttttccagaa tcgaatcaca tgctaactct tgataaccac gatccattaa
     2281 gtggcattcc tggttataaa agtattccgg ttgaattaga accatcaaat taattcgcct
     2341 cctcatttct tctgctgtca tccgcacagc agaagaattt ctcattgact attatcgcgc
     2401 aatttgctca cattgattaa attaaaccac acactataag atataaacct ctgcctacag
     2461 ctgtaagaaa ctccgctcag tactgaagtg ccagtcctat ttcctctttt ctctagcctg
     2521 ttatattaag catactgatt aacgattttt aacgttatcc gctaaataaa catatttgaa
     2581 atgcatgcga ctacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg
     2641 tctgtaattg tggttgtctt attactgacc cttacgttga tagccatttt ttcggcaatt
     2701 gatcagctgg gtatttcaga aatgggtcgc attgcaagag atcttacaca
//
LOCUS       DAPHJE010000245         2719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPHJE010000245 DAPHJE010000000
VERSION     DAPHJE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.248"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(58..2712)
                     /gene="chiA"
                     /locus_tag="RH222_004472"
     CDS             complement(58..2712)
                     /gene="chiA"
                     /locus_tag="RH222_004472"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HDV3799377.1"
                     /translation="MPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAKA
                     NDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQGT
                     VPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKSNDASNPWRYVRAA
                     TATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVA
                     WKGQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYG
                     NPGSCSVKPDNNGGAVTPVDPTPETPVTPTPDNNEPSTPADSGNDYSLQTWSGQEGSE
                     IYHVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLVRTATAAELSQYGNPTTCEID
                     NGGVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWS
                     ASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEE
                     LNNAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGT
                     PKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYG
                     MQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLKVL
                     DFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLS
                     DAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKS
                     DAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTN
                     LSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          630 a          721 c          651 g          717 t
ORIGIN      
        1 ccaaaagtag cccattgaca aaaaatgcgg cgatactgga aggtatcgcc aacacattta
       61 ttgcttagta aacggcgcga agatttcgct aaatgcgtac ttcggtgcct gttctttagt
      121 caggccgtgg aattccggag acaggttagt gccgcccggc aggtcgcgcg cgattgacca
      181 gataccaacc ataccgagat tacgcttctg cgcatcctgc atgaccagac gagcatcaga
      241 gagatagaac acctcgccct gaacgtcgtt cacgccaacc atcggcgtgg tacccatcat
      301 agcgtcaatt tctgcatcgc tcttattggg atggaggcct ttcaattgtg aatgcaggtt
      361 ggcaatcgca gacgttgcac acttaccgtg aatgttctgg ccttcggtat ttgcagactg
      421 acaaatcgcg ttaccgtagt ccattgtcat cacgttcaca cccgccagct caacaccttt
      481 cgctttggca tcgctcagga cattcatccc ttccggcgtc aggccagtcg gcagaattgg
      541 caaggtgtac cagatagcaa tatctttgcc ttctgacttc catttatcct gcactttctt
      601 cacagcaagg ttacgacgtt caatagatgc ctgatccgca acccaggtgc cttcgatatc
      661 gaagtccagg actttgaggt tcaggttatc aacgatgtca taataatgct gcatcagatc
      721 gtctacgttc ttacaggaag cagccagcgg agcgttgtta gcaccaccga tagacagcat
      781 cacatcgccg ccagcctcac gcagagcttt gattttgctg tactgagcgt aattctgcat
      841 accataagcg gtaccccatg tcggcagaca ggtgttcgca tctttactca ccacaaacgc
      901 cagcgtgaag tggttgacgt tatgattctt agccagcgca gccaaatccg ggatcgtatt
      961 cagcgtaaag tcaacatacg gtgcataaac gtgtttcggc catgctttct tcggcgtacc
     1021 tacacgttct ttggttccgg tccagtcaac aaaaacacgc cacgggttgc tgtcagaagg
     1081 agaaactttc tgcactttac tctgagaaat gtagttcaca ccgttaaagc gaatcagcgt
     1141 atcgctggca taaagcgttt ctggattaaa ctgcggcgca ttattcagct cttcgttgct
     1201 atagctctga gccttaccta acggcttcca cgggcggcta ttgctaccgg tggcgttttg
     1261 gttcgcaacc agagcagggt tgtcactttg cgtccagaac agcgcttcat aagcctgccc
     1321 atcaacaaca acgcgatcac ctttcacgta cacagtggat gcggaccatg ctttcgccgg
     1381 ttcgtatttc ttccacgggt tatcgccgcc cgggacaaag ccccatgcgt cttgatcgac
     1441 agaagtttta taatgtgcat cgttataatc tacgatgctg tccgcgctgt acgctttgct
     1501 ggcctggaaa ccatccgcaa caatgacgcc gccgttatca atttcacagg tagtcgggtt
     1561 accgtactga ctcaattccg cagcggtagc tgtacgcacg agacgccatg ggttattgga
     1621 gttttcagcg ctggtacccc gtgggcaatc tttagaccca acccaccagg cgttcttgta
     1681 aacattacca ttgaaaataa catggtaaat ttcgctacct tcctggccgc tccacgtttg
     1741 caatgagtaa tcgttaccgc tatccgctgg tgttgatggc tcgttgttat ccggggttgg
     1801 ggtcaccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat tattatccgg
     1861 cttaacggaa caggagccag ggttaccata ctggctgatt tctgttgccg ttgccgcgcg
     1921 aacataacgc catgggttac tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
     1981 gcctacccac caggcgttct tataaatgcc gccgttatag ataacgtacc atgtagaaga
     2041 accttcctga cctttccagg ctactacagc atagttgtcg tttgacttat cagctggggc
     2101 tggctgaaca tcaggagccg gtttaacgtc cggtgccggt ttcacatccg gtgccggttt
     2161 cacatccggt gaaggctgtg gtgctgaagt acaggactgt ggattactgg tttctgagat
     2221 ttccgttgcc gtagcggcac gaacataacg ccatgggttg ctggcatcat tgcttttcgc
     2281 atcacccgga cagttagaag aggctaccca ccaggcattt ttatataccg caccattaaa
     2341 gacgacatac caggtctgac cgccctgctg tttattccag gcaactacag aagaattaga
     2401 cggaacagtg ccctgtgctg gtgtagccga accgccatta gccggcgtat tggatgcagg
     2461 cgtatttgaa tttgaagaag atgagctgcc gctcttttcg caggaaagtg tattgccaaa
     2521 ctgactaatt tcagcagctg ttgcggtacg ctttaaacgc cacgggttag ttgcatcatt
     2581 tgctttcgct tttcccgggc aatttgtaga agaaacccac caggcatttt cataaatttt
     2641 gccatcgaaa ataacctgat atttattacc accttgttgg ttattccatg cttccattgc
     2701 caatgctggc agagcggaa
//
LOCUS       DAPHJE010000246         2715 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPHJE010000246 DAPHJE010000000
VERSION     DAPHJE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.249"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..1280
                     /gene="bluF"
                     /locus_tag="RH222_004473"
     CDS             69..1280
                     /gene="bluF"
                     /locus_tag="RH222_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV3799378.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQCDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDFYTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1485..2216
                     /gene="bluR"
                     /locus_tag="RH222_004474"
     CDS             1485..2216
                     /gene="bluR"
                     /locus_tag="RH222_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV3799379.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2437..>2715
                     /locus_tag="RH222_004475"
     CDS             2437..>2715
                     /locus_tag="RH222_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDV3799380.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCS"
BASE COUNT          791 a          581 c          591 g          752 t
ORIGIN      
        1 aagaaagaca ccggaagata tgtctgttac cgtcttactc tcacctctcc cattaccctg
       61 gacagttaat gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca
      121 aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtgt gacgtaacag
      181 ggatcttact gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg
      241 ttaaaatgat atatcgggct atatgtcagg atccacggca ctataatatt gttgaactgc
      301 tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc
      361 gcttgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc
      421 agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt gcaaccgaac
      481 aatcaaccta tttcgagatc cctgccgaag attcctggct ttttatcgct gacggatctg
      541 ataaagaact tgatttctat accctttcac caaatataaa cgaccacttt gcctttcatc
      601 caattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg caaaaaaatg
      661 aagatagccc atcagctata gcggttgggc agcgtaaaga cggggaaatc tacaaagcgg
      721 atctcaaaag taaggcgctt gcattcgcga tggcacacgc acttgagctc ggtgataaaa
      781 tgatttcaat caatctatta cctatgaccc tggttaacga acctgacgca gtctcttttt
      841 tacttaatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt gaatttactg
      901 aaagtgaagt catatctcgg tttgatgagt ttgccgaagc gattaaatcg ctaaaggctg
      961 ctggtatcag tgtagcaatt gatcattttg gcgcaggttt tgctggtttg ttactcctgt
     1021 cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat gttcataaaa
     1081 gtgggccacg gcaggcaatt attcaggcga tcataaaatg ctgtacatcc cttgaaattc
     1141 aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa tccgcaggaa
     1201 ttgagatgtt tcagggagat ctgtttgcta aagctaaatt gaatggtatc ccttccatag
     1261 cgtggccaga gaaaaaataa ttttcagcac attctttcac atgacttcag taaatcatcg
     1321 tgaaaatgaa tacgttacgt ttaaatttaa tccacattaa aaataatgtt tcatccaatt
     1381 tagctgaaat cttatttata ctttgtacaa ttttagcgta tattgtacaa agtattattg
     1441 ggtcgtgtac aggcgacgga gatttgtgac cgcaaggagg aattgtggct tattacagca
     1501 ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc tggcaacgcc
     1561 gctacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt gatgaagaag
     1621 acatacaacg catcgaagag atcaagcgtt ggataagtaa tggcgtccct gtaggcaaag
     1681 ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagc cgcctgcaag
     1741 aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg agaattattt
     1801 cactgggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt cctgttcgcc
     1861 agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt gacggcgcat
     1921 taattgaata cgcagcagcc tcgcttttcg aaatgcgccg taagcccggt aaagaagcca
     1981 ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagca tggcgtttat
     2041 cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgcca cgcccggaac
     2101 tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga aggcaaaacg
     2161 aacttttaca acattggggt gagcaaggtt ataaagtcat ctttcatgcc ccctaatttc
     2221 acctttatcc cgccccttag acagaatgtt ataatctgaa cccacaacag cgtttgtacc
     2281 ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag atattccctc tatcacactc
     2341 tgtacatatc acagccaaag gacagaccag attgaagtgg taaaaccgat cgtgcacact
     2401 acgcagtctc acaactaaca agaggttatt atcctgatgg atcaggtcgt tgtttttcaa
     2461 aagatgtttg agcaagtgag aaaagagcaa aacttctcct ggttttattc agaattaaaa
     2521 catcaccgta ttgcacatta catttattat ctggctacgg ataacatcag aattattact
     2581 cacgatgata cggttttgtt attaagagga accaggaacc tgttaaaagt tagtacgacc
     2641 aagaaccctg ctaaaataaa agaggccgca ttgcttcata tttgcggaaa atctacattt
     2701 cgggaatact gttca
//
LOCUS       DAPHJE010000247         2710 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPHJE010000247 DAPHJE010000000
VERSION     DAPHJE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.250"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            185..919
                     /locus_tag="RH222_004476"
     CDS             185..919
                     /locus_tag="RH222_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HDV3799381.1"
                     /translation="MSDHTIPEYLKPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAKLKARGLLS"
     gene            916..1416
                     /locus_tag="RH222_004477"
     CDS             916..1416
                     /locus_tag="RH222_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV3799382.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            1490..2062
                     /locus_tag="RH222_004478"
     CDS             1490..2062
                     /locus_tag="RH222_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDV3799383.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRIQSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            2366..>2710
                     /locus_tag="RH222_004479"
     CDS             2366..>2710
                     /locus_tag="RH222_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV3799384.1"
                     /translation="MARPLPLNKDLIVCVPSNYSNTSRGKFFENFCADILRRQSYRID
                     GMEVRKSGMEIDIQATHTPSNEKLYVECKFMQQKVDSSVVDLAFSQAFRLRVKKIALF
                     SISDLGKDAQSTL"
BASE COUNT          729 a          655 c          723 g          603 t
ORIGIN      
        1 gaacagaggt gaacagtaaa atataaaaaa acaaacagta agccggtttt tcctgcgacc
       61 ttttcctggc ttgccggtgt gaggatgagt ctcctgtgtc agggctggca catctgcaat
      121 gcgtcgtgtt gctgtccggt gtacgtcaca attttcttaa cctgaagtga cgaggagccg
      181 gaaaatgtct gaccacacta tccctgaata tctgaaaccc gcactggcac aactggaaaa
      241 ggccagagcc gcccatcttg agaacgcccg cctgatggat gagaccgtca cggccattga
      301 acgggcagag caggaaaaaa atgcgctggc gcaggccgac ggaaacgacg ctgacgactg
      361 gcgcacggcc tttcgtgcag ccggtggtgt cctgagcgac gagctgaaac agcgccacat
      421 tgagcgcgtg gcacgccggg agctggtaca ggaatatgac aatctggccg tggtgctgaa
      481 ttttgaacgc gaacgcctga aaggggcgtg tgacagcacg gccaccgcct accggaaggc
      541 acatcatcac ctgctgagtc tgtatgcaga gcatgagctg gaacacgccc tgaatgaaac
      601 ctgtgaggcg cttgtccggg caatgcatct gagcatcctg gtacaggaaa atccgctcgc
      661 caacaccacc ggccatcagg gctacgtcgc accggaaaag gctgtcatgc agcaggtgaa
      721 atcatcgctg gaacagaaaa taaaacagat gcaaatcagc ctcaccggcg agccggttct
      781 ccggctgacc ggactgtcag cggcaacact cccgcacatg gattatgagg tggcaggcac
      841 accggcacag cgcaaggtgt ggcaggacaa aatagaccag cagggagcaa agcttaaggc
      901 cagaggactg ctgtcatgat ttactgcccg tcgtgtggac atgttgctca cacccgtcgc
      961 gcacatttca tggacgatgg caccaagata atgattgcac agtgccggaa tatttattgc
     1021 tctgcgacat ttgaagcgag tgaaagcttt ttctctgact gtaaagattc aggaatggaa
     1081 tacatttcag gcaaacagag ataccgcgat tcactgacgt cagcctccgg cagcatgaag
     1141 cgcccgaaaa gaatgcttgt taccggatat tgttgtcgga gatgtaaagg ccttgcgctg
     1201 tcaagaacat cgcggcgtct gtctcaggaa gtcaccgagc gtttttatgt gtgcacggat
     1261 ccgggctgtg gtctggtgtt taaaacgctt cagaccatca accgcttcat tgtccgcccg
     1321 gtcacgccgg acgagcttgc agaaagcctg catgaaaaac aggaactgcc gccagtacgg
     1381 ttaaaaacac aatcatattc gctgcgtctg gaatgagggc tgccggttaa caccggccgt
     1441 cgccgcacac cgtattttta ttcttcagca tgatgagaaa gagataacga tggaaagcac
     1501 agccttacag caggcctttg acacctgtca gaataacaaa gcagcatggc tgcaacgcaa
     1561 aaatgagctg gctgcggccg aacaggaata tctgcggatt cagtcaggag aaggcagaaa
     1621 cgtcagtcgc ctggacgaat tacgcaatat tatcgaagtc agaaaatggc aggtgaatca
     1681 ggccgccggt cgttatattc gttcgcatga agccgttcag cacatcagca tccgcgaccg
     1741 gctgaatgat tttatgcagc agcacggcac agcactggcg gcggcactgg caccggagct
     1801 gatgggctac agtgagctga cggccattgc ccgaaactgt gccatacagc gtgccacaga
     1861 tgccctgcgt gaagcccttc tgtcctggct tgcgaagggg gaaaaaatta attattccgc
     1921 acaggatagt gacattttaa cgaccatcgg attcaggcct gacgcggctt cggtggatga
     1981 cagccgtgaa aaattcaccc ctgcgcagaa catgattttt tcgcgtaaaa gtgcgcaact
     2041 ggcatcacgt cagtctgtgt aaaattcccc gaaaatcctc ccgtttttac tgaaaaaagc
     2101 catgcatcga taaggtgcat ggctttgcat gcgttttcct gcctcatttt ctgcagaccg
     2161 caccattccc ggcgcggtct gagcgtgtca gtgcaactgc attaaaaccg ccccgcaaag
     2221 cgggcgggcg aggcggggaa agcactgcgc gctgtgggtg cataaccaaa agttttgatg
     2281 tcgtaaatat gcgcaagata ctggtttctg aaactcgagg ttgatatctt acttgtcaaa
     2341 aggatatgag ataaggagtg tataaatggc caggccgtta ccactgaata aagatttgat
     2401 agtttgtgta cctagtaatt attccaatac ctcgagaggg aagtttttcg agaatttttg
     2461 cgctgatata ctccgccggc aatcgtatag aattgatggt atggaagtca gaaaatctgg
     2521 catggaaatt gatattcagg ctactcatac accatcaaat gaaaagctgt atgtagaatg
     2581 taaattcatg cagcaaaaag ttgattcatc tgtggttgat ttggcatttt ctcaagcatt
     2641 taggttgaga gttaaaaaaa ttgcactttt ttcaatttct gatttgggta aagatgctca
     2701 gtcaacgctt
//
LOCUS       DAPHJE010000248         2687 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPHJE010000248 DAPHJE010000000
VERSION     DAPHJE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.251"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1888)
                     /locus_tag="RH222_004480"
     CDS             complement(<1..1888)
                     /locus_tag="RH222_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV3799385.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTN"
     gene            complement(1932..2615)
                     /locus_tag="RH222_004481"
     CDS             complement(1932..2615)
                     /locus_tag="RH222_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV3799386.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          627 a          634 c          593 g          833 t
ORIGIN      
        1 gattagtcac cgtctcagtc aggttagaga tgttggtggt gttagtggca atattggaag
       61 tgttattggc gatattggtg gtattcgtcg ccacagcatc gttggtggtt ttcagctgag
      121 agccgttaac agcatcagtg ctgtcagccg tcaggtcggc atctttaacg ttggtgattt
      181 tgctggtggt atcctggccg tgagctgcac tgaatgcctt tttagtttcg ttccagagca
      241 gggcatcagc ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg gtggtattgg
      301 tggcaatatt tgccgtattc gtggcaatgt tgctggtatt agtagcgata ttggcggtgt
      361 tggcttcgac atcgtcattg gtagctttca gttgggaacc gttaaccgca tccttactgg
      421 aggctgaaat cgtgccatcc gcaacatcgg tgattacgct ggcagttggg ctactagtac
      481 catgagcagc gctgtatttg cctttggttt ggtcccattg cagggtgttt tcatcaagta
      541 cagcgagcgc agcccctacg ttatttttgc taccattttt taaattgtag gttggtgctg
      601 taacagtacc gtcatcaaca tctactgcag cccctcctcc aagccttttt gctaccgagt
      661 cgctaatggc ataaagctgt gaaccattaa tggcatcgta actgtcagac ttgatcgcgc
      721 catttttcac attaacgatc ttgcgtttta atgaactatt gccgacagag acggtattgt
      781 ctaaatccgc aatggaatct gcgcccagtg cgacagagtt aataccgttg gcactgctat
      841 tactaccaag cgcaagactc ttatcaccag ttgcattact ggctgtgcct aaggcaatgg
      901 tgtttaagcc tctcgactgg ctaccattac ccagagcgat ggcatccacg ccatcagctt
      961 tgttgccctg gcctatggcg attgcattat ctgccagagc ctggctgcca tttcctaatg
     1021 cgatagtatt gactttatcg gcaaggcttc cgaaccccat tgccatgcta ttagtccctt
     1081 ttgccttcgc attattgcca atagcagttg cattttcagc ggcttcggtc tcagcaccta
     1141 tcgcaatagc gttcgctgcg ctggcaagtg attgtctgcc aaatgcgagg ctgtcagtac
     1201 cgctagctac actgctacgc cccagggcaa ttgcgttttc ttcagatgcg acgctacttg
     1261 ctcccagcgc cattgaatac gcttttgacg cattggcggt gtcgccaaga gcaatactca
     1321 taatctcagt agctttagtg gcatttccca gggcaatggc gtttgctcct tccgctttgg
     1381 aagagtcccc catagcaata gacaatttac ccaatgcttt tgaataacgg ccgagggcca
     1441 ttgagtattg gcccaatgaa tacgaagagg cacccagtgc tatacttcta tcgccttctg
     1501 atattgcact aaccccaaag gccattgatg caccgccaat cgcatgggtt tttgacccga
     1561 tggcagagct atattggcct tcagctatag cgtcataacc cacagcagta ctggaaccac
     1621 tggtgttcat aaaagtattt gctttcgccc ctttgcctat agcaacccag ccgtcgccag
     1681 ctgatccact accgtaatca acaccctgac cgttgtcatt cccggcattc gccaatgccc
     1741 caaacgacga caacattcca cccgcaacca gcgcagaaat taacagctta ctgcgcccag
     1801 atttcttgcc acggcttttt gccgtttcgc tggtaacagt ataattccct gtcgcagggt
     1861 tccagataac tttaaatatt ttgttcatta atgatgctcg ctattccttg ttataagcgt
     1921 actaactgca tttatttttt gcttcttttc tgccgatttt cagcagaggg aagattgtcg
     1981 aactgaatat tttccatgtt gatggctaac ttcagatatc ccaggttatt gggcgataca
     2041 cccttcaggg tcaaattaat gtcttgccat tgacctggta ataatttcaa tgtggatttc
     2101 attgatgtgt tgaaaagtaa ttgttgccac cgggcaaact ctttggcatc gacaggtttt
     2161 tcaccacccc atgtcgcatt aaaaccaata tttgagatag gtgtacttaa taagttgacc
     2221 agtgaaatat gaataacact gccattctct gtggcgtcaa tggataacac tcgacccgct
     2281 aaagccaccg aaccattgtt caggatgaaa tacttcgttt ttgctgggtt aaactcggta
     2341 aaaataaaag tgttttgttg atctttaacg gatttaatta cgtgagttaa agtctttgta
     2401 ctgcttatta tgccagtttg ttgtttttga atagtttcca gttgactctt tagctgtgct
     2461 tgttgttttt caagtagatc aattttattg ttatgttctt cttgttgttt caataaacta
     2521 ctttctggct tgccttcttc aatgtagtca catcccgtta atgcgcagag aactgaaaca
     2581 gtgacagcac tgaaaaaaat cttcttcaag ttcatgtaaa aatccttatt tcctccgacc
     2641 agcgatcaat gccggtagag ttgtccattg tattattggt gatctat
//
LOCUS       DAPHJE010000249         2675 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPHJE010000249 DAPHJE010000000
VERSION     DAPHJE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.252"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            122..1351
                     /locus_tag="RH222_004482"
     CDS             122..1351
                     /locus_tag="RH222_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3799387.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            1811..2443
                     /locus_tag="RH222_004483"
     CDS             1811..2443
                     /locus_tag="RH222_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3799388.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGDKINELFIRSHSVQH"
BASE COUNT          774 a          537 c          519 g          845 t
ORIGIN      
        1 aggtttgcaa ccactaaccc accaatagag gggtagatag ggcgttaatc tcccatactt
       61 aacctggttt atggtaaatt gccctccatt ttgtttaatt tgtagatgat acgttcagat
      121 aatgtctaat atttggtcta aagaagaaac tctgtggagt ttcgcgctct acggcacagc
      181 cgttggtgca ggcacgctct tccttcctat tcagttaggt tcggcagggg ctgtggtcct
      241 gtttattact gctctggtcg cctggccttt aacatattgg ccacataaag ccttatgcca
      301 gttcatcctc tcatcgaaaa catcagcagg tgaagggata acgggcgcag taacacacta
      361 ctatggcaag aagattggta atctgattac cacgctgtac ttcatcgcct tttttgtcgt
      421 cgtgttgata tatgcagtgg caattaccaa ctcacttacg gaacagctgg caaagcatat
      481 ggttattgat cttcgcatcc gtatgttggt gagtctgggt gttgtattaa tcctgaatct
      541 catttttctg atgggacgcc atgcaaccat tcgggtaatg ggttttttgg tattcccatt
      601 gattgcctat ttcttatttc tttccattta ccttgtcggt agttggcaac ctgatctatt
      661 aacgacccag gtagagttca atcagaatac ccttcaccag atatggatat cgattcccgt
      721 gatggttttc gcctttagcc atacgcccat tatttctacg tttgccatag acagacgtga
      781 aaaatatggc gaacacgcta tggataaatg caaaaaaatt atgaaagtcg cttatctcat
      841 catctgcata agtgtactgt tctttgtctt tagctgcctg ctttctattc caccttcgta
      901 tattgaagcg gctaaagaag aaggggtcac cattttatcg gcgctttcta tgctgccgaa
      961 cgccccagca tggctgtcaa tttccgggat tattgtcgca gtagttgcga tgtcgaaatc
     1021 attcctgggt acgtattttg gcgttattga aggtgccacg gaggtcgtca aaacaacact
     1081 acagcaggtt ggtgtaaaga aaagtcgtgc atttaaccgc gcactatcaa ttatgttggt
     1141 atcgctgatc accttcattg tttgttgcat taacccgaac gcgatttcga tgatttacgc
     1201 gatcagcggc ccgctcattg ccatgatact tttcatcatg cctacgttgt caacgtatct
     1261 gatcccggcg cttaaaccct ggcgttccat cggaaatctg attacgctga tcgtgggtat
     1321 cctgtgcgta tcggtaatgt tctttagctg atatccattg tgaatggttc aaaattgcta
     1381 caattttgaa ccattccagt tttcataatg ttcattcgtt attattccgc actatcactt
     1441 ccctcatcat atcctagtat tatcagttag ttaaacacac aaagagatct gaacatctta
     1501 aataagacta tttaagatgc ataacttaga ttcgcaagat atcttccggt cattcaaaaa
     1561 caatttgcga tatagatatt ctcataccat gagatagtta tctgaaccac cctcaaccag
     1621 gtaagattaa atcagtgaaa cataatcata tttaataacg agaaatgcat tttaaaatgt
     1681 cttaatcaga attatagcaa atacagatta atcgcaattt ttcccagcaa aattacaaaa
     1741 tcatggtgta ttgacatcat caatcaccaa tgaaattatg caattatata cggataggga
     1801 ggttcttaac atggggcttt gtagtcgtta taaaagtctt acatgcaata gttgctcaat
     1861 gcattgccaa ataatgccag aagagtcacc gcgtttgcag tactgcgcaa actcgtgttt
     1921 ttgtatgtgg cccgaagaaa gttcatattt taatcgtggt gtggtagaag gtatcttaac
     1981 aaaaaaccac aatgcgagat taagcggata tatttttgtc gatttttcag taagtttttt
     2041 acgtctattc cttgaaaagg actggattga ctatcttgca agtactgaca tggggattgt
     2101 tttagtcagc gaccgcaata tgcaatcgtt agctaattac tggcgaaaac acaactcagc
     2161 aatatctgct gtcatatata atgatgatgg tcttgatgtt gccaatgaaa agatcagaca
     2221 actatttatt ggtcgttatt tatcatttac cagaggtaac acattaactc agatggaatt
     2281 caccataatg gggtatatgg tttctggtta taatccatat cagattgctg aagttctgga
     2341 tatggatatc cgtagcatct acgcgtacaa gcaacgaatc gaaaagagaa tgggtgataa
     2401 aataaacgaa ttatttattc gttcacattc ggtccaacat tgatactcaa cgaccaggca
     2461 gaatcatact ctgtttatac gggcatttat tgaaatgtcc gtattttata aataggccgt
     2521 cccctcaaga gtaaacacca tagtatttaa aaccatcaca cgaaaaattc agaagcatta
     2581 cgaaatatga atttacaaac aatagtggca taaatgttaa ccatgttaat ttacgtaaag
     2641 ttttacgttg caacattaaa gcctcatttc aatca
//
LOCUS       DAPHJE010000250         2672 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPHJE010000250 DAPHJE010000000
VERSION     DAPHJE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.253"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(8..487)
                     /locus_tag="RH222_004484"
     CDS             complement(8..487)
                     /locus_tag="RH222_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV3799389.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(569..1390)
                     /locus_tag="RH222_004485"
     CDS             complement(569..1390)
                     /locus_tag="RH222_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3799390.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSER
                     YAYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(1611..2021)
                     /locus_tag="RH222_004486"
     CDS             complement(1611..2021)
                     /locus_tag="RH222_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799391.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(2037..2525)
                     /locus_tag="RH222_004487"
     CDS             complement(2037..2525)
                     /locus_tag="RH222_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799392.1"
                     /translation="MKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGA
                     CPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGD
                     GCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAA
                     VN"
BASE COUNT          630 a          676 c          660 g          706 t
ORIGIN      
        1 tctccattca gtcgatgatg cgcataatgg cgctgcattc cggatgctgc agggcgtaat
       61 cccgcaaccg gtaataatgg accgtcatgg cataattctc cgtacgacag gcatgatgac
      121 tgtacgtcat cagacaggcg gcaataccgg cggcttccgg gctcatttct gcgcggttac
      181 cgttcatggc attgaacagt acccatgttt cgtcatcatc gtcatccggt tcgggtgcca
      241 taaatgcccc gccgttgttc agggtgtaca gattccagat accaccgcag tagtcttcgc
      301 acagacggtc catccagccg aagacacggg gctccagggt cacccactgt ggaatgaggc
      361 caaaatgctg cggccagaag ctgatgcgct gttcatcagg gacgaggctg gcaaccaact
      421 gaggcggatt attctctggt gttgtagctg aataaattgt gggggtattc tgagaaacgg
      481 ttttcatgag ataactcctt aaataacaaa taaatgtgtg tttttcagga acgaaagctg
      541 aatgattccc tcactcaggg aaacggcatc agcgcaggct ctccagcatc gtttctgcca
      601 tcacccacaa tgcgcgattg agcttaatgt cggtatcaat gctgtgaatg gcccgggtat
      661 ggatacgttt tccttttgca ctgcgaccgg aaatcccgcc tttcagcata ttctcctgga
      721 tggtctgata tgcgctccac aggtccttac cgtaatcctc ccggcgtcgc ggcgtcagaa
      781 tgtcggcggt ggtgacgggc tgatgttcgt caccataacg gtaagtcagt gccgcctgtg
      841 ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg catggcatca cgcttctcct
      901 ctatccggtc aaacacgccc accacctcgt aagccccttc gataactttc tccactacat
      961 ttccccggtg tggaacacgc acttccccca gagactggcc acagacgcac ccgttctggc
     1021 agacgaacct gaagtaaccc ggcagcatct ggtagctgga ggtaccgtca tgagagttga
     1081 gcagaataat ttcagggaca tgttctccgt ttatctctcc ggcccgccgc agacgcagca
     1141 tgtgttttgt gtatccccgg cggcccgggt cgcgcacacg ggtctggcag gcgaagaagg
     1201 gctgaaagcc ctcccgctgc aggctttcta gtacggtgat ggtgggaatg tacgcatagc
     1261 gttcactgcg ggaggtatgc cggtcttccc caaaaatact gggcacatag tgcatcagtt
     1321 cttcatgtgt cagcgggcgg tcacggcgta tctggtttgc agcataacca aaacgacttg
     1381 ctaatcgcat actttgctcc ttatcggtgg ttaagattta ctggtgcaat aaatgaaaaa
     1441 agccgcgtct cccggagaag acgcggcctg acgggtgaaa tgaataaggt ttattgtctg
     1501 agaggccttt cactggcgag ccacgcatta agcggctttc cggtaacaat ggctggggct
     1561 gtatttacct ttgatattaa taatgtgcag agaaacgcat ggtttactcc ttagatttta
     1621 agtttgtcct gggctacaac gacaggtttt ccggatgtgt cgatgactcc taccgtcggt
     1681 acgccatttt tgatttcgaa gaacggtttg ttgctacctg agcacagttg attgtccccg
     1741 tattcaatca tttgtgcttt cccggttttc aggttaacta ccgagacagt gtagtcgtag
     1801 caccaggcac caccacgttc aagcacgaaa acatagctgt tatctttata gttgaactga
     1861 ttctttgtct ggcaattcat accgtcaatt tcggtacagg aaaacgcttc tgtagtatta
     1921 acgtatttga tgttatttgg cagttttact tgtttcgcag tagcgaagac agagaacaga
     1981 atagttacag caacgagtaa agttttgata aaagttttca tgatagactc cttaaattag
     2041 ttaaccgcag ctacgtagtt tttcaccaga tacgcgaatt catgcacgcc aatttcaccg
     2101 aaccaaacca ggtaggccgg gtcatctaaa ccaaagtaac ggtagccaac aacttcatgg
     2161 gtttttgggt tatagagtgt tacgataaag tcccagccgc aggtattggc tttgcaaccg
     2221 tcgccctgga accagactgt accgttaatg tttactggcg tgatacctcc accagggcca
     2281 tccatatagc cgcctttacc gaacatgccg ctcagtccga ccggttttaa tgctttgtcg
     2341 atagcggctt tgtaggtagc gtcaccttcg tacaactcgt acgttgatgg acacgctcca
     2401 ggttgttcag cccaggcttc cgggttacaa ctaagagctt gttttacggt agccgcttct
     2461 gcgttaaagt taaagcccgt gaacagaacc gccagaaata acataagggt atgtaaacgt
     2521 ttcattgtaa atattcttcc gttgtaaatg gcgctggaaa atttctcatc agtgaaacct
     2581 tccggaagtt tccccccctt gtatgatgta tatcgccagt attttatttc atcccggctg
     2641 taatttttat attccccggc acgtgcaata ac
//
LOCUS       DAPHJE010000251         2663 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPHJE010000251 DAPHJE010000000
VERSION     DAPHJE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.254"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(169..1349)
                     /locus_tag="RH222_004488"
                     /pseudo
     CDS             complement(169..1349)
                     /locus_tag="RH222_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            1936..2165
                     /locus_tag="RH222_004489"
                     /pseudo
     CDS             1936..2165
                     /locus_tag="RH222_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2508..2651
                     /locus_tag="RH222_004490"
     CDS             2508..2651
                     /locus_tag="RH222_004490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799393.1"
                     /translation="MSLDPLACGHDNVKQSKIVALTVSLGDKKAQYSRWQPDNLTVDA
                     ETK"
BASE COUNT          657 a          656 c          667 g          683 t
ORIGIN      
        1 ataaaaaagt aacctcataa aaaatcctgt actcacaggg tgatgcggga taactgacgt
       61 aaggagtgct gcagggattt gatggtccct tgtgctgata tgaatacgga tattttatac
      121 agaatacttt tcagtacaag cgactgtatt acagtctgcc atatatgatt acctgtgatc
      181 aggaagaata agtggctgag gtttcaaact gtctgccggt atattcctct ctcccttaaa
      241 aaccataaca ggttagttat cttcgtctgt cagcggattg aatggcggta tattttcagt
      301 acaaataccg gtcatccact gaaaaatacc tgcgaaatga cgggcactga tttttctact
      361 gacgggctga tgagacgtta tgtcactggc ggtaataatc agtggcacac tgtagcctcc
      421 ctgcacatga ccataatgat gaacaggatt ggcactgtcg ctgaccgaca gcccaaggcc
      481 agaaaagtaa agcatggcga aatgacgtga gtaccggcga aggataccat caagctggcc
      541 aagaaagtta tccagtttac tgatgctggc gaggtaacag gcaatttttc ggggatactg
      601 ctccaggtaa ttattcggct aggagttaag gctgtcacac ggatttggat gagaacccat
      661 catgtgcagg aaaattatct tcggagagga tgtatccgcc agcgcacgtt ctgtttcctg
      721 taacaacagt ttgtcatctg ttttacggga agcgaagctg cctttcttga ggaaagtggc
      781 atgctccgca tcagaagcaa taacagagat gcgtgtgtca tgctccccca gttttccttg
      841 attggatatc caccatgtgc tgtatcctgc ttttgctgcc agcgccacca cattgttgcc
      901 ggaatcaggg ttctgctcat agtcataaat cagtgtccgg ctcagggaag acactgtact
      961 ggaggctgtc gaggtataat cgtcaataaa taaaccgggt gccgtattca gccacggtgt
     1021 ggttggtacg ggatagtcat acactgacat ataatccctg cgcacactct caccagtgac
     1081 aataacaatc gtatcatata acggtgctcc cggctggatt ttccagttgt cagccccgtg
     1141 ctactcagtt gttcataacg ctgcatttca cgtaatgtgt cagttgtccc cacaactgtt
     1201 cctttaacca tccgcaatgg ccaacggttt acagagcata atacgaatag ccgtagagcc
     1261 agccagttac ggtggtcgcg atggtgtgtc cgccagaaaa tcaccacgaa taccagaatc
     1321 gcggcactga ccagaaaatg ataaacagga aacacggggc ggttcacttt gctttctcct
     1381 gtagtgtttt ctgtaggttc tgaaccggtt ctagcggggt tgtgaggctg atggcaatcg
     1441 ccgggctctt cctgcatgtt acactggtac tgcaagaggg ggccttcgct cctctgacat
     1501 tactcagtat tttcactact acaccccatc tgccatccat aacaaccgat tcaccccctc
     1561 gcgaggcgtt gttgcccgtc acccggacag acaccgttat ggacttctct tgttgcacga
     1621 tcgatcaatt gttatgcatg ctgacaccac aaccccggtg ggtatgaccg atgcctttct
     1681 cactctcttc ccggtcatcg tcggccttcc ccgttattgt ggcgtgtcag caccctcgac
     1741 ggcatttcga tggctgctca gtgttcactc acgttacggc ccgcataacc cgttgacttc
     1801 ctgaagaagt cgtttcaggg agagattcag cccttttgtt acctcctgga ccgcttcttg
     1861 tccttccagt cggagcgaga gttagccggg gcggactttc accgacggaa agatcgtgca
     1921 tttcaaggca cacacaacaa tatctgcgag cgtgctatcc gtccggtcgt tatgggacga
     1981 aaggcctggt tgttcgctgg ttcactggtg gccgggaacc gcgcggcaca gataatgagc
     2041 cttctgggaa ccgcagtctg gagccacatg cttggctgac ggacgtcctg acgcgtctgc
     2101 cggagtggcc ggaggagagg ttggctgagt tactgcctct tgagggcttt accttcttcg
     2161 ggtaagtgat acctgccgtc aggtgtttgt gcaccgggcc ataacctgca gttgggaatt
     2221 gaacgcctga caacaggaaa tgagctagag cagtaaacac gcgggctgac aatgggtcag
     2281 aatatgagtt agcccggcgt tattcctggg gtcagtactt cagatctagc ctccggtacc
     2341 agactagcta gcccctacgc aaccaaataa atcaaccatc gtcagagtgc aatcgttcat
     2401 atacactgtc ggtgatgcgc taaaaaatgc gttaatgttg agttcctcga acccttacca
     2461 attaagggac atagccagat acgcaactgg ctgatgcaag cagtctaatg tctttagatc
     2521 cgctggcctg cgggcatgac aatgtcaaac agtcaaagat tgtggcgttg acggtcagtc
     2581 ttggggacaa aaaagctcag tattctcgtt ggcaaccaga taatttaaca gttgatgctg
     2641 aaacgaaata ggcatcttgg ttc
//
LOCUS       DAPHJE010000252         2516 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPHJE010000252 DAPHJE010000000
VERSION     DAPHJE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.255"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            146..1078
                     /locus_tag="RH222_004491"
     CDS             146..1078
                     /locus_tag="RH222_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV3799394.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(1119..>2516)
                     /locus_tag="RH222_004492"
     CDS             complement(1119..>2516)
                     /locus_tag="RH222_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HDV3799395.1"
                     /translation="ALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKKKGEHAYDMTLS
                     YKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPITMLMHGNVIPA
                     LAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFSFGEG
                     QFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGESES
                     KFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEW
                     SLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLLQKS
                     EPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVATETAK
                     QLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNGQE
                     MSEEEFMSRAGRFVH"
BASE COUNT          652 a          551 c          526 g          787 t
ORIGIN      
        1 aaaataaagc catccctaat aattgagata atgggatttt ttctaatgtg gatagatatg
       61 aattattttt ctccttaagg atcatccgtt atttgggtcg ttttctgcta aaatctgcgc
      121 cttgcgcttc tttagcgcac tgtctatggc taaccttttg aataaattca ttatgacgag
      181 aatactcgct gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt
      241 ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta ttgctacctg tgccagtcat
      301 ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc
      361 ggtattactg catctgaacc cacacctgca atgggaagtt cacgggctgg aagggttaag
      421 taagaagaac tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact
      481 gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc aacagctggc
      541 ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat atgccgttta tgaagcgtta
      601 ttcccgcgct tatttattac gtcatccgga gcgacgtggt aaagatgttg aaaccactcg
      661 gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg ttgaaggctc
      721 ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact tgttgccacc
      781 aaaggcggca ggcattgcga tggcgctaaa tgtactgggt aaacaattcg ataaactgct
      841 taatgttacg ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg
      901 taaactgacg cgaattgtcg ttcatgtgga tttacagcct attgccgatg agttacatgg
      961 cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga actcactgtg
     1021 gcaggaaaaa gaccgacttc tgacgtcgtt gatgtcatca cagcggcagg acaagtaaat
     1081 tttgctcgcc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca
     1141 cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta
     1201 taacgcagtt gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa catctgacct
     1261 aaggtcatca tcccgctgat ttgtttatca gcccgctttt gcgctttttc cgcatccatt
     1321 ccttcagata aattaagctg ttttgcggtt tctgtggcga cattaagcgg taacttcaca
     1381 tcaaaattga gcgatttaat atctttgttt gtggatgatg aagatttggc tgggtcggca
     1441 atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccattt taccggttgt
     1501 ttaatggtcg gctcactttt ttgtaataac ggtaaatact ctttgaacaa tgcagcattc
     1561 acttcttgca gagcaacttc atcgtttgct aactcagggt gtgcagcaag ctgcttctgc
     1621 attgcaatgt tatactggat gataaattgg cgcaccgctg agggatcaat agattcagca
     1681 atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg
     1741 taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcat ggtttttgca
     1801 acgaaagcat cgatttgggc aacgtcttcc ccgtgattga taatgttaat ctgattaaat
     1861 ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgcgccag agaatcaatg
     1921 gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcgact
     1981 ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt aaactggccc
     2041 tcaccaaaag aaaactttcc atactctgca ggggcaacat ctaatgtcgt agagaatgag
     2101 ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat
     2161 agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag tgctgggatg
     2221 acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat
     2281 acaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcgaa tgtcatttgc
     2341 atttgaaaac gagaattaaa aaaacctttg tcaaaatttt tataacttaa cgtcatatcg
     2401 taggcatgtt cacctttctt tttagcatcg ttaaaatctt taataaactt ttcgacacca
     2461 ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc agattacggc taacgc
//
LOCUS       DAPHJE010000253         2498 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPHJE010000253 DAPHJE010000000
VERSION     DAPHJE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.256"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..2365
                     /gene="adiA"
                     /locus_tag="RH222_004493"
     CDS             98..2365
                     /gene="adiA"
                     /locus_tag="RH222_004493"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV3799396.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHSDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          631 a          681 c          614 g          572 t
ORIGIN      
        1 atttcaaaaa tgtttgtttt tcacgcgctt tacagcccga aaaggccgga agatacttgc
       61 ccgcaacgaa gattccttca taaccgggta agcaatgatg aaagtattaa ttgttgaaag
      121 cgagtttctc catcaagaca cctgggtcgg taacgccgtt gagcgtctgg cagatgcttt
      181 aagccagcaa aatgttaccg tgattaaatc cacctccttt gatgatggtt ttgccattct
      241 ctcttcaaac gaagccattg actgcctgat gttcagctat caaatggaac attcggacga
      301 acatcaaaac gtcagacaat tgatcggtaa gcttcatgag cgccaacaaa acgtgccggt
      361 cttcctgttg ggcgatcggg aaaaagccct cgccgcaatg gaccgggatc tgctggagct
      421 tgtcgatgaa ttcgcctgga ttctggaaga taccgccgac tttatcgccg gacgcgccgt
      481 tgccgcgatg acccgctacc gccagcagct gttgccgcca ctgttcagcg cgctgatgaa
      541 atatagtgac atccatgaat attcctgggc agcgccaggc caccagggcg gcgttggttt
      601 taccaaaaca cccgccggac gtttctacca tgactactat ggtgaaaatc tgttccgcac
      661 cgacatgggc atcgaacgaa cttccctcgg ttctttgctt gaccatactg gcgcatttgg
      721 cgaaagcgaa aaatatgccg cacgcgtatt tggtgccgat cgctcctggt cggtagtcgt
      781 cggtacttcc ggctctaacc gcaccatcat gcaggcttgc atgaccgata acgatgtcgt
      841 ggtcgttgac cgtaattgcc ataaatccat cgaacaaggc ttgatgctga caggcgcgaa
      901 accggtctat atggtgccaa gccgcaaccg ctacggcatt atcgggccaa tctatccgca
      961 ggaaatgcaa cctgaaacct tgcagaagaa aatcagtgaa agcccgctga ccaaagacaa
     1021 agccgggcaa aaaccgtctt actgcgtggt gaccaactgc acctatgacg gcgtgtgtta
     1081 taacgctaaa gaagcgcagg atctgctgga aaaaacctcc gatcgtctgc actttgacga
     1141 agcctggtac ggctatgcac gtttcaaccc gatctatgcc gatcactatg ccatgcgcgg
     1201 cgaacctggc gatcacaacg gtcctaccgt tttcgccacc cactccaccc acaaactgct
     1261 gaatgcgctg tcacaggctt cttatattca tgtacgtgaa ggtcgtgggg cgattaactt
     1321 ctcccgcttc aaccaggcct acatgatgca tgccaccacc tccccgctgt atgccatctg
     1381 cgcatccaac gacgtggcgg tgtcgatgat ggacggcaac agcggcctgt cactgacaca
     1441 ggaagtgatt gacgaagcgg ttgatttccg tcaggcgatg gcgcggctat ataaagagtt
     1501 caccgctgac ggtagctggt tcttcaaacc gtggaacaaa gaagtcgtca ccgacccaca
     1561 aaccggcaaa acctatgact ttgctgacgc accaaccaaa ctgctgacca ccgttcagga
     1621 ctgctgggta atgcatccgg gcgaaagctg gcacggcttc aaagatattc cggataactg
     1681 gagtatgctc gacccaatta aagtcagcat ccttgctccg ggaatgggtg aagatggtga
     1741 actggaagaa accggtgttc cggcggcgct ggtcactgcc tggcttggtc gccacggcat
     1801 tgtgcctacc cgcaccactg acttccaaat tatgttcctg ttctctatgg gcgtaacccg
     1861 tgggaaatgg gggactctgg ttaacaccct ttgctccttc aaacgccact atgacgccaa
     1921 cacaccgctg gcgcaggtga tgccggaact tgttgaacaa tatcctgaca cttacgcgaa
     1981 catggggatt cacgatctgg gtgacaccat gtttgcctgg ctgaaagaaa acaaccctgg
     2041 cgcacggttg aacgaagcct attccggcct gccgatggcg gaaatcaccc cgcgtgaagc
     2101 gtacaacgcg attgtcgaca acaatgtcga actggtatcc attgaaaatc tgccaggacg
     2161 catcgcggca aactcagtta tcccgtatcc gccaggaatc ccgatgctgc tgtctggtga
     2221 aaacttcggc gataaaaaca gtccgcaagt aagttattta cgctcgctgc aatcctggga
     2281 ccaccatttc cctggatttg aacacgaaac tgaagggact gaaattattg acggtattta
     2341 ccacgttatg tgcgtgaaag cgtaaccact attccgctga aggcgtaatt gtttaaataa
     2401 cattacgccg cctggcctta ggccttttga gtatggcaac gttttcataa aaattgctgc
     2461 aaacaaaaat gtcatacttt ttgcgcggtc ccaccccg
//
LOCUS       DAPHJE010000254         2394 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPHJE010000254 DAPHJE010000000
VERSION     DAPHJE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.257"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>2394
                     /locus_tag="RH222_004494"
     rRNA            <1..>2394
                     /locus_tag="RH222_004494"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          623 a          525 c          755 g          491 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcacgcttag gcgtgtgact
      181 gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg
      241 ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac
      301 ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg
      361 actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg
      421 gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg
      481 gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa
      541 taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg
      601 aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg
      661 aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata
      721 gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat gcaccgaagc
      781 tgcggcagcg acgcttatgc gttgttgggt aggggagcgt tctgtaagcc tgtgaaggtg
      841 tgctgtgagg catgctggag gtatcagaag tgcgaatgct gacataagta acgataaagc
      901 gggtgaaaag cccgctcgcc ggaagaccaa gggttcctgt ccaacgttaa tcggggcagg
      961 gtgagtcgac ccctaaggcg aggccgaaag gcgtagtcga tgggaaacag gttaatattc
     1021 ctgtacttgg tgttactgcg aaggggggac ggagaaggct atgttggccg ggcgacggtt
     1081 gtcccggttt aagcgtgtag gctggttttc caggcaaatc cggaaaatca aggctgaggc
     1141 gtgatgacga ggcactacgg tgctgaagca acaaatgccc tgcttccagg aaaagcctct
     1201 aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg tagagaatac
     1261 caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt aacttcggga
     1321 gaaggcacgc tgatatgtag gtgaagcgac ttgctcgtgg agctgaaatc agtcgaagat
     1381 accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga aagtggacgt
     1441 atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggtcagc gcaagcgaag
     1501 ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
     1561 ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag gctgtctcca
     1621 cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg gcaagacgga
     1681 aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga tgtgtaggat
     1741 aggtgggagg cttagaagtg tggacgccag tctgcatgga gccgaccttg aaataccacc
     1801 ctttaatgtt tgatgttcta acgttgaccc gtaatccggg ttgcggacag tgtctggtgg
     1861 gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa
     1921 tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact gcgagcgtga
     1981 cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat ggaagggcca
     2041 tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca agagttcata
     2101 tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc
     2161 ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag aacgtcgtga
     2221 gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg ctcctagtac
     2281 gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg gcactgcccg
     2341 gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact tgcc
//
LOCUS       DAPHJE010000255         2387 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPHJE010000255 DAPHJE010000000
VERSION     DAPHJE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.258"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..322
                     /locus_tag="RH222_004495"
     CDS             <1..322
                     /locus_tag="RH222_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDV3799397.1"
                     /translation="HKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            354..1646
                     /gene="sctN"
                     /locus_tag="RH222_004496"
                     /pseudo
     CDS             354..1646
                     /gene="sctN"
                     /locus_tag="RH222_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            1636..2052
                     /locus_tag="RH222_004497"
     CDS             1636..2052
                     /locus_tag="RH222_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDV3799398.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLKSLLDKKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYVRLKTRIKKTFKSDASSRRA"
     gene            2070..2299
                     /locus_tag="RH222_004498"
                     /pseudo
     CDS             2070..2299
                     /locus_tag="RH222_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
BASE COUNT          789 a          429 c          543 g          626 t
ORIGIN      
        1 ccataagttt tcatattctg gagaaattaa agcaattgtg atttcgcctg aaatagagga
       61 cagaatcaga gatggtgtca ggcctaccgc tggcggtact tttcttaatc tcgatgcatc
      121 cgaagctgag atgattctgg ataattttaa actggcactt tctggaatca atattcctat
      181 taaagacatt attttactgg gatctgtgga tattcgtcgt tttatcaaaa aacttattga
      241 atctagttat cgagatctcg aagtactttc gtacggggaa ctaacggaaa atgttcccgt
      301 taatattctg aaaactattt aggataatgt cttgcgatat ggagcatgcc ggtatgaaaa
      361 gattaaaatt attaaataaa tacagctact tacatagcat aaacggctct ctgattgaag
      421 cagaacttga cgacgtttca gtgggtgaag tatgcgaaat ttatgcaagc tggcaagcaa
      481 atgaacgtat agccagggct caggtcgttg gctttcgcaa tggaaagaca ttattaaacc
      541 ttataggcag ctcagtgggg cttacacgta ctgcagtttt gaaaccaacg ggagagcagt
      601 taaccataca gatcagtgat gcctttcttt gttcggtttt gaatgcctct ggtcaaatta
      661 tggaaaggtt tgtcccgaac cctccaggcg atagaggaaa tttacgcctg attgatgaac
      721 tgccaccgtc gtatcaggaa cgtcgagtta ttaatacacc actagagaca cgcataagag
      781 tcattgatgg cgtactgaca tgtggcattg gtcaacgtgt tgggattttt gcatcggctg
      841 gctgtggcaa aacggtttta atgcatatgc tcgtaaacaa tactgaagct gatgtgtttg
      901 ttattggctt gattggcgaa cgtggtaggg aagatacgga atgcgcggaa tcgatgaaac
      961 aatcagtaaa tgcggcaaaa tgcgtattgg tttatgccac ctctgatttc tcgtcagtag
     1021 atcggtgtaa tgccgccctg atggcgacaa ccgtggcaga gtattttaga gatcgaggaa
     1081 agagagttgt actttttatc gactcaatga cgcggtatgc atgagcacta cgtgatatga
     1141 aactggcagc gggtgaacca cctgccagag gtggttatcc tgcatctgtt tttgacagtt
     1201 tgcctcgctt actggagagg ccgggaccaa cactaaaagg aagtatcacc gcgttttata
     1261 ccgtgttact ggaaggggaa gacgaatcag accccctggg agatgaaatt cgctcaatcc
     1321 tcgatggcca tatctatctg agtcgcaaac tcgctgggca aggtcactat ccggcaataa
     1381 atgttctaaa aagtgtgagt cgagtatttg gtcaggtcac tgatgaaaaa cacagagata
     1441 atgctgcacg ggtaaggaaa atcctcacaa ctttggaaga tctaaaggtt tttattgact
     1501 taggggaata tcgcgctggg caaaatgctg aaaatgattt cgctatgaat gcacggccta
     1561 aactcacaaa ttggttgaaa cagtcagtta atgaaaaaat gccaatgagt gaaactctaa
     1621 aagagcttga acgaattgtt aagtaaagtt aatcgactta tcagaagaac agcgcagagc
     1681 ctggctgcat gtgaagcatc attacaaaag ttaaatgcag aaaaagaaaa gttagccgaa
     1741 aaggaacgcc tgtatgatat gcaacttaaa aacctgaagt cactgttaga taagaaggag
     1801 ttactcggcg aagttgtatt tcgacaggat atattttact cgttacgtaa agttgcagtc
     1861 attcagcagc agatcgctga gataaatctt gaaaagcaaa aaatagcaga gcggagaaaa
     1921 atacttaaca aggaaatagt tcaacagcaa gcacaaagaa aacactggtg gctaaaagga
     1981 gaaaagtacg ttcgactaaa aacgcgcatc aaaaaaactt ttaaatcaga tgcttcatca
     2041 agacgagctt gaacaggagg aaaaatataa tggacgaagt caagaaaatt gaacacggta
     2101 aaattttaaa cacagacaca cccgataatt ctgcttcgaa tttagacagt ttcttaaaga
     2161 aaaacaaaaa aataaagaaa tgagtgagat tgtcgctgga accattctcc agttattacg
     2221 tcagactcag gatagccaat tttctacatt ggggaaaaag aatgggcaaa tgaagtctat
     2281 tgacaacaaa tcatctctcc aggatgacag gaattcctct ctttctttgc aaacaaatgg
     2341 aacagaacaa ttttctacaa agaaaattct caatatgact tctcgag
//
LOCUS       DAPHJE010000256         2341 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPHJE010000256 DAPHJE010000000
VERSION     DAPHJE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.259"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..567)
                     /locus_tag="RH222_004499"
     CDS             complement(<1..567)
                     /locus_tag="RH222_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799399.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(662..868)
                     /locus_tag="RH222_004500"
     CDS             complement(662..868)
                     /locus_tag="RH222_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3799400.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          599 a          564 c          627 g          551 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agagaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggt gtggcccgtt tgtcagggcc
      361 ataaatcagc cggtgcaggc tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccaa ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagccattc
      541 gtagcgggtc tgatactgtt gtttcatttt catattcctc acggggatat aacaggcagc
      601 agaatgccgc cggcaggccg gagattacgt ctgctgcggg tgaacggata acggtcagaa
      661 attacggacg ggactgtgca atacgcgcct gcagccaggc ttccacttca cttttcagcc
      721 agcgggagct gcgccccatt ttgatggggg caggaaagcc cccatctttg atgagtttgt
      781 aaaaccactt atcggttaag ccggtaagtt gagtgataaa cgccatgtcg accatctggt
      841 catccatcag cgaaacaggg gtagccatga gaatgttcct ccggatgttg ttaaaccagc
      901 cggtgtggtc atgaaaacag cgcgccacct tcactggcat aacaaccggg gagaaacgta
      961 aaaaataatc gggaatgtgg ctgtccggag ataaacgggg ggattgcgat ggcagagccg
     1021 tagcggaatc ccggggggtt gttgcgaaaa gatgcttccg cacgcacgcg cgataaagga
     1081 gggaggactt tcgtgagaca aggggaaaac tagcgttctg ataattgcag agttgttttc
     1141 ggaggtggtt acagtgagtc agcccgttcc tggcgaggag gagactgata tcaccgatgg
     1201 gataaggctg tctgattggt attataaata taagaagtca tcagagagta acgtaaagga
     1261 tttgccagag taagctaaga gagtaaatct ctccggtaac agagcgctgt gaagcattct
     1321 gtatcagagt gcaaatacat gaacgctgat gcgtaaaatc agcagtgaca cgccagctcc
     1381 ccctgtgccc attcctgtat aagacatgaa ctgagcgtat taatgctcct gctctgagac
     1441 tgagccggtg agtgaggggc tgagaaatcc ctcttgacac agctctaaac cagaaatact
     1501 tccccggttg ttgcggtgcg tccggatccc cgataacgga acactaaggc tgtattgcgg
     1561 gacaccgggt aagtggcagc ctgtaatgtg ttctgtcaac gccagaaaaa atgatttttc
     1621 acacgcgtta tagactcact cctgtggacc tggaagcccg taactctgcg gttgcccacc
     1681 gactgacgga actgaccgca aactttacga gtgcgtgcag ggcttcagtc tggtcacctg
     1741 agcgttcggt aacggaggcg cgggcggtaa taggctgatt ttcaggtatt accggcttac
     1801 agcgtgtggt tgatattgag gggctgtgag tttcgcgtat ttgtcagaaa cccagacgag
     1861 ttttcctgta tgggacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg
     1921 aaggagaacc tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat
     1981 gaaccgttat ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg
     2041 accacaataa tcagtgccag cagtatggta acgatacggt ctgtattccg gtaatagccg
     2101 aaggggtcga cagaaccaca atacgggcaa cacactgtat tatgagtaat cgcgtttcca
     2161 cagtccctgc acttcttttt gcgtatcacc atcactccct cctcacagcc ttacccgtaa
     2221 ccggtttttt acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgaccg
     2281 ttcgggttat cacagagtgc ctgagacagt gtcctgccgg aggtcacttc agggactctg
     2341 c
//
LOCUS       DAPHJE010000257         2324 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPHJE010000257 DAPHJE010000000
VERSION     DAPHJE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.260"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..70
                     /gene="ariR"
                     /locus_tag="RH222_004501"
     CDS             <1..70
                     /gene="ariR"
                     /locus_tag="RH222_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDV3799401.1"
                     /translation="ILKADVIRKTLEIVLRYTADDM"
     gene            183..431
                     /locus_tag="RH222_004502"
     CDS             183..431
                     /locus_tag="RH222_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799402.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            763..2286
                     /locus_tag="RH222_004503"
     CDS             763..2286
                     /locus_tag="RH222_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3799403.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAIINGEIVPFYQPVVNGLEGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          639 a          482 c          501 g          702 t
ORIGIN      
        1 tatcctcaaa gctgatgtga ttagaaaaac actggaaatc gtgttgcgat acacagctga
       61 tgatatgtaa cttctaatta ttaagtataa gtttatagaa aactcattca tcttattttt
      121 gtctgtcgct ttagactata cacagcataa ttttattggg ttaatatttc tacgaggctg
      181 acatgaacaa ttcaatccca gagagattta tttttcaatg tgctttgttt aaaaatctcg
      241 agagggaggt gttcatgacg catgggtatg ttgatagtca tattattgat caggctttac
      301 gcttgcgttt aaaagatgaa accagtgtga ttctttctga tctctatctg caaatcttgc
      361 agtacattga aatgcataag acgacactaa cggatatcat tattaatgac agggaatccg
      421 tgctctctta gaacttgagt aaggttatag aataaacatc agctgtatca tcatgctggt
      481 gcaaagtgag catttcaggc gttatgcttt cttaatatat ccgcaatatc agttgtcaag
      541 aatggagagt tctcgttctc cattcttgat gcctgatatc ccacctaatt tatttaatcc
      601 tgcctacatt atgctcaata attaatgggt tagatcaaga tggagaaaat tttatcttcg
      661 gcgtggatat ttcctatgct ctctaccatg ctctctgtag atgccagcgc gagcgaatga
      721 atggcatttt catgtataaa accctttggc cgggacaagc aaatgcgcaa tacactcata
      781 cccatcctcg ttgcgatatg cttgtttata accggtgtcg ctattttaaa catccagctc
      841 tggtattccg caaaagcaga gtacctggcg ggagcgagat atgccgccaa caatatcaat
      901 catatacttg aagaagcgtc acaggcgact caaacagcgg ttaacattgc cgggaaggaa
      961 tgcgacctcg aggagcaata tcagcttggc actgaagcag ctctgaaacc tcacctgcgc
     1021 acaatcatca ttctcaaaca gggaatagtc tggtgtacat ccctgcctgg gaatcgggtc
     1081 ctgttgtctc gtattcctgt tttcccggac agtaatttac tgttggctcc ggcaatcgac
     1141 accgttaata gattacctat cctgctctat cagagccaat ttgcagatac gcgcattttg
     1201 gttacgataa gtgatcagca tattcgcggg gcgcttaatg tacccttgaa aggggtaagg
     1261 tatgtattac gcgtggcgga tgacattatt ggacctacgg gtgatgtgat gacgcttaat
     1321 ggacattatc cctataccga gaaggttcac tccacaaaat atcatttcac tattatcttt
     1381 aactcgccgc cactctttag cttctacaga cttatcgata aaggctttgg gattttgatc
     1441 tttattctgt taatcgcctg cgccgctgcc ttcctgcttg ataggtattt caataaaagc
     1501 gcaacgcctg aagagatcct gcgacgggct ataattaatg gggagatcgt ccctttttac
     1561 caacctgtgg taaatggtct ggaaggaaca ttgcggggag ttgaggtgtt agctcgctgg
     1621 aaacaacccc acggtggata tatatcaccc gcggcattta ttccacttgc tgaaaaatcc
     1681 ggattaatcg ttccgcttac gcaaagcctg atgaatcagg ttgccagaca gatgaacgct
     1741 atcgcgagta aactgccgga aggttttcat attggaatta attttagcgc ctcgcatatt
     1801 atttcgccga cgtttgtcga cgagtgctta aattaccgtg atagttttac ccgccgcgat
     1861 ttaaacctcg ttctggaagt caccgagcgt gagccattaa atgttgatga aagtctggtt
     1921 cagcggttga acattctgca tgaaaatggt tttgtcatcg cgctggatga tttcggtact
     1981 ggctactcag ggctttctta tctgcatgac ctgcatattg attatatcaa aattgatcac
     2041 agtttcgttg gccgcgtaaa cgcagaccca gaatcaaccc gaattctgga ttgtgtattg
     2101 gatctggcgc gtaaactttc gatcagtatc gtcgctgaag gtgtcgaaac gaaagaacaa
     2161 cttgactatc tgaaccaaaa taatatcaca tttcagcagg gttattattt ctataaacct
     2221 gttacataca tcgacctggt caaaattatc ctttctaaac cgaaggtgaa ggttgtggtt
     2281 gagtgaaaat tgatcaataa ggccatggtg cggtgtaatt atag
//
LOCUS       DAPHJE010000258         2279 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPHJE010000258 DAPHJE010000000
VERSION     DAPHJE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.261"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..685
                     /locus_tag="RH222_004504"
     CDS             2..685
                     /locus_tag="RH222_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV3799404.1"
                     /translation="MTPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGN
                     VGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRR
                     RGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIK
                     LNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIT
                     DFMTARGYH"
     gene            complement(734..2233)
                     /gene="pitB"
                     /locus_tag="RH222_004505"
     CDS             complement(734..2233)
                     /gene="pitB"
                     /locus_tag="RH222_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV3799405.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          602 a          590 c          560 g          527 t
ORIGIN      
        1 aataacacct ccattaattg ccgttattgg tagcgatggt tcaggcaagt caacggtgtg
       61 tgaacatctt attaccgttg tcgaaaaata tggtgctgcc gaaagagttc atttaggaaa
      121 acaggccgga aatgtcggtc gtgcagtgac aaaattaccg ttgatgggaa aatccttaca
      181 taaaacaatt gaacgaaatc aggtgaaaac agcaaaaaaa ttgcctggac cagttccggc
      241 gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata tgcttgcctg
      301 tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa ttcctggcgc
      361 ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct catggctggc
      421 aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc tggtcatcaa
      481 actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac gggaatcgct
      541 ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac tggttgatat
      601 cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga ttacggattt
      661 tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc aggaccgggc
      721 attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca gaaagaaaaa
      781 ttgccgccgg taaagtaaat acccacgcca tcaggatgct ggttaccgtt ttacgctgta
      841 acccaccgcc gtccaccacc atcgtccctg caactgcaga cgagaggacg tgtgttgtgg
      901 agacgggcat cccaatataa ctggcaagac cgatagacac tgccgccgtc atttgtgccg
      961 ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg gtcatcgcta
     1021 cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc atgatgatcc
     1081 acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc agcaggttct
     1141 ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca gagatgcaca
     1201 gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa ctttccatat
     1261 tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc ggatgacagt
     1321 gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt tccatcgcaa
     1381 tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg gtgacggcat
     1441 cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag ccagcagggg
     1501 caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg ttcgcgccgt
     1561 gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag aatggcggtt
     1621 tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg tcacgctttt
     1681 tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg accaggccga
     1741 cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg tttaacgcat
     1801 ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata atcgcaccaa
     1861 tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg ttccagataa
     1921 tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc atattcagca
     1981 acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg cccaataaca
     2041 cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc atggcacgag
     2101 tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt gcttcgtaga
     2161 acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca aggccaacaa
     2221 ataaatttag catatattag attacgccat tttgaattta cgaacggacg cattatcag
//
LOCUS       DAPHJE010000259         2197 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPHJE010000259 DAPHJE010000000
VERSION     DAPHJE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.262"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..533
                     /locus_tag="RH222_004506"
     CDS             30..533
                     /locus_tag="RH222_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3799406.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(579..995)
                     /gene="rraB"
                     /locus_tag="RH222_004507"
     CDS             complement(579..995)
                     /gene="rraB"
                     /locus_tag="RH222_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV3799407.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1157..2170
                     /gene="argF"
                     /locus_tag="RH222_004508"
     CDS             1157..2170
                     /gene="argF"
                     /locus_tag="RH222_004508"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV3799408.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          555 a          525 c          554 g          563 t
ORIGIN      
        1 taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa
       61 tgcgtcgcct gacgttgcag gataatcctg ctatcgcccg cgtcattcgt caggtatccg
      121 ccgaatacgg tcttaccgct gataaaggct acaccgtcgc cgatccgaat cttgacgaac
      181 tgtatcaagt gtatagtcag cctggtcatg catattgggt ggttgagtac gagggtgaag
      241 tggtcggcgg cggtgggatt gcgccattag caggtagtga atcggacatt tgcgaactgc
      301 aaaagatgta ttttcttccg gctattcgcg gtaaagggct ggcgaaaaaa ctggccttaa
      361 tggcgatgga gcaagcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt
      421 ttttaaagga agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcgc
      481 ttggctgcac gggccatgtc gattgtgaag tgcggatgct acgcgaactc taaaaaaatg
      541 cccgctagtg tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
      601 tcttcatcga caaaatcttc atcgtcgccg tcttcgccgt tgggatcttc aaagtaagtg
      661 ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg
      721 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
      781 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
      841 tcaactgctg ctttttccag ggtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
      901 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgtgtttct
      961 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     1021 gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgacaa agatttatgc
     1081 tttagacttg caaatgaata atcatccata taaattgaat tttaattcat taagatgtta
     1141 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     1201 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa
     1261 aagcggcaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     1321 ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccagggtg ctcgcgttac
     1381 ttatctcggc ccaagcggca gtcagattgg tcataaagag tcgattaaag acactgcccg
     1441 cgtgctgggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgtcga
     1501 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
     1561 cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttacccggta aagcattcaa
     1621 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
     1681 agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccgga
     1741 agctgcgctg gttacggaat gccgcgctct ggcacagcaa aatggcggga atattacgct
     1801 gacggaagat gtagctaaag gagttgaagg tgctgacttt atctataccg atgtatgggt
     1861 gtcgatgggt gaagcaaaag agaaatgggc agaacggatt gcattactgc gtgattatca
     1921 ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct
     1981 gcccgcgttt catgacgacc aaacgacgct tggcaagaaa atggcggaag agtttggcct
     2041 acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtatttga
     2101 tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaaatt
     2161 aaataattaa tatctctatc aagatgccgg gaaatac
//
LOCUS       DAPHJE010000260         2058 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPHJE010000260 DAPHJE010000000
VERSION     DAPHJE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.263"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..218
                     /locus_tag="RH222_004509"
     CDS             117..218
                     /locus_tag="RH222_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799409.1"
                     /translation="MRTYNLNSLLPSQMQKCTCDSLHPTFDLCGGEA"
     gene            215..670
                     /gene="ybcN"
                     /locus_tag="RH222_004510"
     CDS             215..670
                     /gene="ybcN"
                     /locus_tag="RH222_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HDV3799410.1"
                     /translation="MNLPQDGIKLHRGNFTTIGQQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATAVWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            670..840
                     /locus_tag="RH222_004511"
     CDS             670..840
                     /locus_tag="RH222_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinE from lambdoid prophage DLP12"
                     /protein_id="HDV3799411.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRSHG"
     gene            833..1123
                     /locus_tag="RH222_004512"
     CDS             833..1123
                     /locus_tag="RH222_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDV3799412.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            1120..1482
                     /gene="rusA"
                     /locus_tag="RH222_004513"
     CDS             1120..1482
                     /gene="rusA"
                     /locus_tag="RH222_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HDV3799413.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1479..1619
                     /locus_tag="RH222_004514"
     CDS             1479..1619
                     /locus_tag="RH222_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HDV3799414.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1705..>2058
                     /gene="quuD"
                     /locus_tag="RH222_004515"
     CDS             1705..>2058
                     /gene="quuD"
                     /locus_tag="RH222_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HDV3799415.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLE"
BASE COUNT          541 a          472 c          532 g          513 t
ORIGIN      
        1 attgaaaatt ataggttggc ggttacttaa atttgatttt ccaaaatcag atcgtcataa
       61 ttatcgcatc gaagcctgaa cacctccggt gacttctgcg ctaaacgggg acgtttatgc
      121 gcacatacaa tctaaactct cttctccctt cacagatgca gaaatgcacc tgcgattctt
      181 tgcatccaac gtttgacctc tgcggaggtg aagcgtgaac ctcccacaag acggcatcaa
      241 attacatcgc ggtaacttca ccactatcgg tcagcagatc cagccttatc tggaggacgg
      301 aaaatgcttt cgcatggtgc ttaaaccgtg gcgcgagaga cgcagtcttt cccagaatgc
      361 actcagccac atgtggtaca gcgaaatcag cgagtacctc atcagcaggg ggaaaacgtt
      421 cgctactgca gtatgggtaa aagatgcact caaacacaca tacctcggtt atgaaaccaa
      481 ggacttggtt gatgtcgtaa ccggcgaaat cactactatc cagtcgttac gccatacctc
      541 cgatcttgat accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcgatgaa
      601 tattggctgc cacctgacta ttccgcagag ctgcgagttc cagctgctgc gcgacaagca
      661 ggaggcgtaa tggctacacc gcttattcgt gtcatgaacg ggcacattta cagagtacca
      721 aatcgtcgta agcgtaaacc tgagctgaag ccttccgaaa taccaacact gctcggatat
      781 accgccagct tggttgataa aaaatggttg cgactggcag caaggaggag tcatggctga
      841 tttgagaaaa gcagcgcgtg gtcgggaatg ccaggtaaga atccctggcg tatgtaatgg
      901 caaccctgaa acgtctgtac tggcacatat ccggctggct ggattgtgcg gtaccggtat
      961 caaaccgcca gacctgattg ccaccattgc atgttctgcc tgccacgacg aaatcgaccg
     1021 ccgcacacat tttgtcgatg ctgcatatgc aaaagaatgc gcgctggaag gtatggcgag
     1081 aacacaggtt atctggctga aagagggggt tattaaggcg tgaataccta cagcatcaca
     1141 ttaccctggc ctccgagcaa taatcgctat taccgccata atcgcgggcg cacgcacatc
     1201 agcgcagagg ggcaggcata ccgcgataac gtcgcccgaa tcattaaaaa cgcaatgctg
     1261 gatatcggcc tggctatgcc tgtgaaaatc cgcattgagt gccacatgcc ggatcgccgt
     1321 cgccgtgacc tggataatct gcaaaaagcc gcttttgacg cactcactaa agcaggtttc
     1381 tggctggatg atgctcaggt cgttgattac cgcgttgtga agatgcccgt taccaaaggt
     1441 gggaggctgg aactgaccat caccgaaatg gggaatgaat gatgtttgag ttttatatgg
     1501 cagaacttct tcgccaccgc tgggggcatc tgcgcttata tcgtttcccc ggttctgttt
     1561 tgaccgatta ccgaatactg aagaattacg ccaaaaccct gacaggagca ggagtatgaa
     1621 gtcagagata acaatcaact aatactgttt tgttgatttt tgcttgtgat tggcgttctg
     1681 gtctgatttt tgtggagtaa gttgatgcgt gatattcaga tggttcttga gcgttgggga
     1741 gcgtgggcgg ctaataatca tgaagatgtg acctggtcgt ccattgccgc cggttttaag
     1801 ggattaattc cttcaaaagt aaaatctcgc ccgcaatgtt gtgacgatga cgcgatgatc
     1861 atttgcgggt gcatggcccg tctgaaaaag aacaacagcg atttgcacga tttattagta
     1921 gattattatg tagtcggtat gacattcatg tcactggcag gtaagcattg ctgctctgat
     1981 ggttatatcg ggaaaaggtt acagaaggct gagggcataa ttgaagggat gttaatggca
     2041 ttagatatcc ggttagag
//
LOCUS       DAPHJE010000261         2026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPHJE010000261 DAPHJE010000000
VERSION     DAPHJE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.264"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1499
                     /locus_tag="RH222_004516"
     CDS             <1..1499
                     /locus_tag="RH222_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3799416.1"
                     /translation="ATDPAGNRLPDPELHPDSALSMWPDNRIARDAHYLYRYDRHGRL
                     TEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAK
                     RVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVET
                     ATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAEYDEWGNLL
                     NEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYP
                     LNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSA
                     PNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTT
                     QLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1496..2026
                     /locus_tag="RH222_004517"
                     /pseudo
     CDS             1496..2026
                     /locus_tag="RH222_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          577 a          489 c          534 g          426 t
ORIGIN      
        1 atgccacaga cccggcaggt aaccgcctgc ccgacccgga gctgcacccg gacagcgccc
       61 tcagcatgtg gccggataac cgtatcgccc gtgacgcgca ctatctttac cggtatgacc
      121 gtcacggcag gctgacggag aaaaccgacc tcatcccgga aggggttatc cgcacggatg
      181 atgagcgcac ccaccggtac cattacgaca gtcagcaccg gctggtgcac tacacgcgga
      241 cacaatatgc agagccgctg gtcgaaagcc gctatcttta cgacccgctg ggccgcaggg
      301 tggcaaaacg ggtgtggcga cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga
      361 aaccgcaagt gacctggtac ggctgggacg gcgaccgcct gaccacaata cagaacgaca
      421 gaacccgcat ccagacgatt tatcagccgg ggagcttcac gccactcatc agggttgaaa
      481 ccgccaccgg tgagcaggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt
      541 ccggcggcga agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc
      601 ggctggaaag tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat
      661 cgtgcggcct gacggtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc
      721 gaaaaatcca cctgtaccac tgcgaccatc gcggcctgcc gctggcgctt gtcagcacgg
      781 aaggggcaac agaatggtgc gcagaatacg atgaatgggg caacctgctg aatgaagaga
      841 acccgcatca gctgcagcag cttatccgcc tgccggggca gcagtatgat gaggagtccg
      901 gcctgtatta caaccgccac cgctattatg acccgctgca ggggaggtat atcactcagg
      961 atccgattgg gctgaagggg ggatggaatt tttatcagta tccgctgaat ccggttcagt
     1021 atatagattc aatgggactg gcatcaaaat atggacactt aaataatggc ggatatggag
     1081 cgagacccaa caaaccgcct acgcccgatc caagtaaatt gccggacata gcgaaacaat
     1141 taagactgcc atatcctatt gaccaggcca gtagtgcgcc taatgttttc aaaacattct
     1201 tcagagcatt aagcccttac gactacacac tgtattgcag gaagtgggta aaaccaaatc
     1261 tgacttgtac gccacaggat gattcccagt atccagggat ggatacaaag acagcaagtg
     1321 attacctgcc acagacaaat tggccaacaa ctcaattacc accaggatat acttgtgcag
     1381 aaccctattt attcccagac attaataaac ccgatgggcc agcaacagca gggatagatg
     1441 atttgggtga aattttagct aaaatgaaac agagaacatc gagaggaata agaaaatgaa
     1501 aagagttttg ttctttttgc tgatgatatt tgttagtttt ggtgttatag ctgattgcga
     1561 aatacaagct aaagatcatg attgttttac tattttcgct aagggtacta ttttttccgc
     1621 gtttcctgtc ttaaataata aagccatgtg gagatggtat cagaatgagg acatcggtga
     1681 gtattattag caaacagaac tgggtacatg taaaaataat aaattcacac caagcggggc
     1741 gagactctta attcgcgtcg ggtcactacg ccttaatgaa aatcatgcca tcaaaggaac
     1801 gttgcaggag ttgataaata cagcagaaaa aacggcgttt ctcggcgatc ggtttagaag
     1861 ttatataaga gcgggtatct atcaaaaaaa gagcagtgat ccagttcagt tgttggcagt
     1921 ccttgataat tcaatcatgg taaaatattt taaagatgag aaaccaactt atgctcgaat
     1981 gacagcacat ctcccgaaca aaaatgagtc ttacgaatgt ttaata
//
LOCUS       DAPHJE010000262         1982 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPHJE010000262 DAPHJE010000000
VERSION     DAPHJE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.265"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(76..1488)
                     /gene="pykF"
                     /locus_tag="RH222_004518"
     CDS             complement(76..1488)
                     /gene="pykF"
                     /locus_tag="RH222_004518"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV3799417.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          501 a          454 c          481 g          546 t
ORIGIN      
        1 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
       61 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      121 cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc tctgcagagc
      181 cagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg
      241 cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag
      301 gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgggtagc
      361 aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      421 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      481 gtcggtacgt tcgcagatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      541 tgcggattca ccagacagca tcactgcgtc agtaccgtcg aggatggcgt ttgcaacgtc
      601 accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc
      661 agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      721 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      781 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      841 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
      901 cttacgaata aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
      961 ctgtttgtct ttttcagcca gtgctggcag agcaatggaa acgccaggca ggttcacacc
     1021 tttgttttcg cccaggtcgc cgttgttcag tactttacag ataactttgt taccttcaat
     1081 ggcggtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc caacagacag
     1141 gtcagtagtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1201 atcagtggtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag
     1261 tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1321 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1381 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1441 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1501 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1561 cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1621 gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1681 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt
     1741 aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat
     1801 tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcac tgtttgcgca
     1861 acgtctggtg ccgaagttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaata
     1921 tatcatcgct caattgctac agttaaaata atttatcgcg ttatttgtct gattttgttt
     1981 ga
//
LOCUS       DAPHJE010000263         1952 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPHJE010000263 DAPHJE010000000
VERSION     DAPHJE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.266"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..748
                     /locus_tag="RH222_004519"
     CDS             44..748
                     /locus_tag="RH222_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV3799418.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            844..1353
                     /gene="dfrA8"
                     /locus_tag="RH222_004520"
     CDS             844..1353
                     /gene="dfrA8"
                     /locus_tag="RH222_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HDV3799419.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            1350..>1952
                     /locus_tag="RH222_004521"
     CDS             1350..>1952
                     /locus_tag="RH222_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV3799420.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAHVKDAIGA
                     SVASKWTAIIHAPVNGVVLGIPDHGFDGKELASYGFGLVLA"
BASE COUNT          470 a          512 c          515 g          455 t
ORIGIN      
        1 gtggtgataa acttatcatc cccttttgct gatggagctg cacatgaacc cattcaaagg
       61 ccggcatttt cagcgtgaca tcattctgtg ggccgtacgc tggtactgca aatacggcat
      121 cagttaccgt gagctgcagg agatgctggc tgaacgcgga gtgaatgtcg atcactccac
      181 gatttaccgc tgggttcagc gttatgcgcc tgaaatggaa aaacggctgc gctggtactg
      241 gcgtaaccct tccgatcttt gcccgtggca catggatgaa acctacgtga aggtcaatgg
      301 ccgctgggcg tatctgtacc gggccgtcga cagccggggc cgcactgtcg atttttatct
      361 ctcctcccgt cgtaacagca aagctgcata ccggtttctg ggtaaaatcc tcaacaacgt
      421 gaagaagtgg cagatcccgc gattcatcaa cacggataaa gcgcccgcct atggtcgcgc
      481 gcttgctctg ctcaaacgcg aaggccggtg cccgtctgac gttgaacacc gacagattaa
      541 gtaccggaac aacgtgattg aatgcgatca tggcaaactg aaacggataa tcggcgccac
      601 gctgggattt aaatccatga agacggctta cgccaccatc aaaggtattg aggtgatgcg
      661 tgcactacgc aaaggccagg cctcagcatt ttattatggt gatcccctgg gcgaaatgcg
      721 cctggtaagc agagtttttg aaatgtaagg cctttgaata agacaaaagg ctgcctcatc
      781 gctaactttg caacagtgcc cgacgcaata atcaccaatt cctcttttga taggtaaaca
      841 acaatgatcg agcttcatgc cattttagct gccaccgcca atggttgcat tgggaaggac
      901 aacgcacttc cctggccacc actaaaaggc gatctggcca gattcaaaaa attgaccatg
      961 gggaaggtgg tcattatggg gcgcaagacc tatgagagct tgcccgtcaa attagaaggt
     1021 cgcacctgca tcgttatgac gcgccaagcg ctggagcttc cgggtgttcg tgacgctaac
     1081 ggcgctatct tcgtgaacaa cgtcagcgac gccatgcggt tcgctcaaga agagagcgtg
     1141 ggcgatgtgg cctacgtcat tggtggcgct gagatattca agcgacttgc cttgatgatc
     1201 acgcagattg aattgacctt tgttaagcga ctgtacgaag gcgacaccta cgttgatctg
     1261 gccgaaatgg tcaaagacta cgagcagaat ggcatggaag aacatgacct tcacacttac
     1321 ttcacttacc gtaaaaagga gcttacagaa tgaccttcgt tgccatttca gatacgcacc
     1381 tgcacaactg gtcgcagttc gcgataccaa cagagtcggg cattaactcc aggctgttgc
     1441 agattcttaa agccattgaa gaagcggctt gcgccgccga ttaccacgcc ccagcgggcg
     1501 tggttccaac cgtctatcac ggcggcgatc tcttccatgt gcgaggttct ttgacccctt
     1561 ctgtgctaaa cgcggtgctc gactttttta agaccatcca ccgtgactac ggcgttcgct
     1621 ttcgcatgat cgccggcaat cacgatctgg aaaccaaaga ctcatgtcct atgggcaatg
     1681 cagctgcggc gcttaattcg ttgccgtttg tggaggttgt atccgaaaaa acgctgttcg
     1741 aggaccacaa agtcgctctg ctgccatggc gcgattccat ggacgatctg cgtgcggatc
     1801 ttgcgcacgt taaagacgcg atcggcgcta gtgttgcctc taagtggaca gcaatcatcc
     1861 acgcaccggt taacggggtt gttcttggca ttccagacca tgggttcgac ggaaaagagc
     1921 tcgcctccta tggcttcggg ttagtgctcg cc
//
LOCUS       DAPHJE010000264         1931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPHJE010000264 DAPHJE010000000
VERSION     DAPHJE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.267"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(4..942)
                     /gene="tdcA"
                     /locus_tag="RH222_004522"
     CDS             complement(4..942)
                     /gene="tdcA"
                     /locus_tag="RH222_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV3799421.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWMLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            1131..1475
                     /gene="tdcR"
                     /locus_tag="RH222_004523"
     CDS             1131..1475
                     /gene="tdcR"
                     /locus_tag="RH222_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV3799422.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSVINNAACHIFSRYLLENKHLFYQYFKISNTCIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            1731..>1931
                     /locus_tag="RH222_004524"
     CDS             1731..>1931
                     /locus_tag="RH222_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799423.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALN"
BASE COUNT          626 a          392 c          347 g          566 t
ORIGIN      
        1 taactaacca acttctatta attgccttcg tctacaccca ttataagatg aatactcttt
       61 ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg accataccgc
      121 ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt gattagaacc
      181 aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt
      241 ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc catttctttg
      301 taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca tccactgttc
      361 gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccag
      421 cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact
      481 taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag
      541 ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat
      601 ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc
      661 ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg taatggattc
      721 ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac
      781 cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga cggccggttg
      841 agttaaccct aattcttttg cagccgagcc gatagaacca cttctaatga cttcctgaaa
      901 gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta
      961 cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac
     1021 aaattaactt tcaatacttt ccagagtatc gttattacaa ttaaatacct ttaaatatca
     1081 acaagttaaa gtataaaaat cagcataaac cctgattttt gtcaaaaaaa atgacgggga
     1141 taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa aaggggctga
     1201 gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta aatttaatga
     1261 gcaagttctc aaattttatt ataaataaac cattttcagt gataaataat gcggcatgtc
     1321 acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat tttaaaattt
     1381 cgaatacatg tattgatcat ctcgaacaat tgattaacgt caactttttc tcttctgaca
     1441 ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca taggcgacga
     1501 atagcatttt gtgttgagga tcacaaaacg aataattgct gatcgccgcg ataaggtcag
     1561 acaaagacaa caagggaaat tttcacagag cttttgatcg gcgtaggcca cagaatgttg
     1621 cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgaaa gaaccctgcg
     1681 aggaaaataa gcatctatta ttgtttgtca tgatgacaag gcaggcatca atgaaaggct
     1741 tcccaattgc gcatattttt cacccttcaa tcccgccaat gcacgcagtg gttaacaatc
     1801 acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcagaaatt gtctccacca
     1861 atgacgttaa taaaatttac agtataagta atgaactgcg gagagtatta tctgcaataa
     1921 ctgcattaaa t
//
LOCUS       DAPHJE010000265         1914 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPHJE010000265 DAPHJE010000000
VERSION     DAPHJE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.268"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            66..1712
                     /gene="mqo"
                     /locus_tag="RH222_004525"
     CDS             66..1712
                     /gene="mqo"
                     /locus_tag="RH222_004525"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV3799424.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT          461 a          470 c          571 g          412 t
ORIGIN      
        1 tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
       61 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
      121 cgatggcggc aaaagcgaaa gcgtctgagg agcaggaaac tgatgtactg ttgattggcg
      181 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
      241 tgaccatggt ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg
      301 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
      361 gcatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
      421 gggcgcacca ggttgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
      481 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
      541 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
      601 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
      661 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
      721 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
      781 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
      841 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
      901 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
      961 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
     1021 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
     1081 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
     1141 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
     1201 atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg
     1261 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
     1321 ggcagcgcgt gcagattatc aagcgtgatg ccgagaaagg tggcgtactg cgtctgggta
     1381 ctgaagtcgt cagtgaccag caagggacca ttgccgcgct cctgggggca tcgccagggg
     1441 cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
     1501 ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga
     1561 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga
     1621 aatacgacaa gccgcaggca gcagatagta cgccgaaacc gcagttgaaa ccgaaacccg
     1681 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
     1741 ggggatgtgg cgtatgctga taagacgcgc cagcgtctta tcaggcaacc ggctcgggcg
     1801 ttagatgtcg gatgcggcgt aaccgcctta tccaatctac ggggtgcagc acttcacagc
     1861 ggtgtaggcc tgataagacg cgccagcgtc gcatcaggca accggcttgg gcgt
//
LOCUS       DAPHJE010000266         1906 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPHJE010000266 DAPHJE010000000
VERSION     DAPHJE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.269"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..423
                     /gene="bssS"
                     /locus_tag="RH222_004526"
     CDS             169..423
                     /gene="bssS"
                     /locus_tag="RH222_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV3799425.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            538..1656
                     /gene="solA"
                     /locus_tag="RH222_004527"
     CDS             538..1656
                     /gene="solA"
                     /locus_tag="RH222_004527"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV3799426.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1704..1817
                     /locus_tag="RH222_004528"
     CDS             1704..1817
                     /locus_tag="RH222_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV3799427.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          495 a          454 c          449 g          508 t
ORIGIN      
        1 gacatgctgt tttattgttc atataaatct taaaagcaga aatttatctg ttcccccctt
       61 taaaaattcg gtgaataccc ttacttattg gtatactgaa gttgcttcag ataaaaacgc
      121 attgaacctc gaataacgtt gtctagtaac acgaattagg gggccatgat ggaaaaaaat
      181 aatgaagtca ttcagactca tccgctcgta gggtgggaca tcagcaccgt tgatagctat
      241 gatgcgctga tgttgcgttt gcactaccag accccaaata agtccgagca ggaagggact
      301 gaagttggtc agacgctctg gttaaccact gatgttgcca gacaatttat ttcgatatta
      361 gaagcaggaa tcgccaaaat tgaatccggt gatttcccgg taaacgagta tcggcgtcat
      421 taacatgaca tgaccatcca cccaaaaggc acctcaccgg tgcctttacc atttctgggt
      481 tgtatatctg cattcagtta attactctgc ttaaaccatg agagcagaga gaaaaaaatg
      541 aaatacgatc tcatcattat tggcagcggt tccgtaggcg ctgccgccgg gtattatgca
      601 acccgcgccg gtttaaacgt gctaatgacc gacgcccata tgccaccgca tcaacacggc
      661 agccaccacg gcgatacgcg attaattcgc catgcttatg gtgaaggcga aaagtatgtc
      721 ccgttggtcc tccgcgcgca aacgctgtgg gatgaacttt cccgccacaa cgaagatgat
      781 cccatttttg tacgctctgg tgtcattaac ctcggcccgg ctgactccgc atttctcgcc
      841 aacgtcgccc acagcgccga acagtggcaa ctcaacgttg aaaagctcga tgcgcaaggg
      901 attatggccc gctggccaga aatacgcgtc ccggacaact acatcggctt atttgagact
      961 gattccggtt ttttgcgcag cgaactggcg attaaaacct ggatccaact ggcgaaggaa
     1021 gcgggctgtg cgcaactgtt caactgcccg gtcaccgcaa ttcatcatga cgatgatggc
     1081 gtaactattg aaacggttga cggtgagtat caggcgaaaa aagcgattgt ctgcgcggga
     1141 acatgggtaa aagacctgct cccggagctg cctgtccagc ccgtacgtaa agtatttgcc
     1201 tggtatcagg ccgatggccg ctatagcgtg aagaataaat tcccggcgtt taccggtgaa
     1261 ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt gaagattggc
     1321 aaacataacg gaggccaggt tatccattca gcggatgaac gtgttccgtt tgcggaagtg
     1381 gtcagcgatg gttcggaagc cttcccgttc ttgcgcaatg tattgccggg tatcggttgc
     1441 tgcctgtacg gcgctgcctg cacctatgat aattcgcctg acgaagattt tattatcgat
     1501 accctacccg gccacgataa tacactgctc attaccggcc tgagtgggca cggttttaaa
     1561 tttgcgtcag ttttagggga aatagctgcc gattttgcgc aagacaaaaa aagcgatttt
     1621 gatttgacgc cattcaggct ttcccgcttc caataatcat cattacggcc tccagaatga
     1681 ggccgttttt acaggagtat ttaatgcgtc ggctgttgca ctatctcatc aataatattc
     1741 gcgagcatct gatgctttat ctttttctct ggggattgtt ggcaattatg gatctgatct
     1801 atgtgtttta tttttagttt tccgcactga cataaactga caaatattta atctcccttt
     1861 catttagcgt gaaaaattga atgagattat tcctgcttct acagca
//
LOCUS       DAPHJE010000267         1855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPHJE010000267 DAPHJE010000000
VERSION     DAPHJE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.270"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1855
                     /locus_tag="RH222_004529"
                     /pseudo
     CDS             1..1855
                     /locus_tag="RH222_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          650 a          359 c          355 g          491 t
ORIGIN      
        1 acttatcgag agttgaataa agcagcacag cgtagcatta cagcgccaga caggatagga
       61 gaaggaaaat catgggttta tcaaagagat acaatgattg aaagaggtga taacagcggt
      121 gttcatcagt atggtcctgc tgaacatttc acccacatta tatctgacaa accgtcccca
      181 aaagatgagt atattgcata tgctattaac attcctgact atgagctggc agccgatgta
      241 tataatatta acgtgacgtc accttccgaa cagcaagaaa catttaaaat actaatcaat
      301 ccagaacatc tacggcaaac acttgagcgt aaatctctta ctgctgttca gaaatcacaa
      361 tgtgaaatca taacccccaa aaaacctggc gaagcgattc ttcatgcttt taatgccacc
      421 taccagcaaa tcagggaaaa tatgtctgag tttgcacgtt gccattatgg gtatatacaa
      481 atccctccag tgacaacttt ccgcgccgac ggaccagaaa ctcccgaaga agaaaagggt
      541 tactggtttc atgcttatca acccgaagat ctttgtacca tccataatcc aatgggagat
      601 ttgcaggatt ttattgcatt ggttaaagat gctaaaaaat ttggtatcga tatcattcct
      661 gattatacct ttaactttat gggaattggg ggtagtggta aaaatgacct ggattatccc
      721 tctgctgata tacgagcgaa gatcagtaaa gatatagaag gtggtatccc tggctattgg
      781 caaggtcagg ttttgattcc attcattaaa gatccagtaa caaaagaacg taaacaaatc
      841 catccagaag atatacatct cactgcaaaa gacttcgaag caagtaaaga taacatctct
      901 aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg aatgcctaaa
      961 acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg tgaaatgcga
     1021 aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca tgaacaaata
     1081 gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa tactaactta
     1141 tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct ggtaacttgt
     1201 cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt aagcgccatt
     1261 gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga tcttcaaacc
     1321 cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg gatattgatt
     1381 aacattaacc acgattttcc taacaatggt aatcttttca atgactttct atttaaccat
     1441 caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag caggccttta
     1501 gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta tgatgggtcc
     1561 acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca gcagctctac
     1621 aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt tgagggtgta
     1681 tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat taatcactct
     1741 cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg tacctggcct
     1801 aacaaattgc aacctggaat atacaaaaac atggggagta atagcgttaa catta
//
LOCUS       DAPHJE010000268         1792 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPHJE010000268 DAPHJE010000000
VERSION     DAPHJE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.271"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1792
                     /locus_tag="RH222_004530"
     CDS             <1..>1792
                     /locus_tag="RH222_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3799428.1"
                     /translation="PVIQEWNQRNKVGETTRLSSLIALKYYQWTVDEDVYLSGRDNEK
                     NILHTILHGAAMIKPEMEEVLVKVLKNRWNEHGTPYFDLMTLILTDLDSYPVWSSLPE
                     YVLQLADLFWYRPLKETGERYHSMDIEDEFGLFRSHHDYYPESPYQTPIYWLLQSQFK
                     KTIDFILDFTNKTTICFAHSHFAKNEIEEVDVFIEEGKFIKQYICNRLWCSYRGTQVS
                     TYLLSSIHMALEKFFLENFKNADSKVLESWLLFLLRNTKSASISAVVTSIVLAFPEKT
                     FNVAKVLFQTKDFFRFDMNRMVLDRTHKSSLISLRDGFGGTDYRNSLHEEDRIKACDD
                     VHRNTYLENLALHYQIFRSENVTEKDVIERQQVLWGIFDKYYNQLPDEAQETEADKTW
                     RLCLARMDRRKMKITTKEKDEGIEISFNPEIDPKLKQYSEEAIKKNSEHMKYVTLKLW
                     ASYKREKDERYKNYGMYEDNPQIALQETKEIIKKLNEEGGEDFRLLNGNIPADVCSVL
                     LLDYFNQLNNEEREYCKDIVLAYSQLPLKEGYNYQVQDGTTSAISALPVIYHNYPMER
                     ETIKTILLLTLFNDHSIGMAGGRYSVFPSMV"
BASE COUNT          613 a          291 c          369 g          519 t
ORIGIN      
        1 tcctgtgatt caggagtgga atcaaagaaa caaagtgggt gaaacgactc gattatctag
       61 tttgatagcc ctaaaatatt atcaatggac tgtagatgag gatgtctatt tatccggaag
      121 ggataatgag aaaaatattc tgcatacgat tcttcatggg gcggccatga ttaaacctga
      181 aatggaagag gttttagtta aggttcttaa aaataggtgg aacgagcacg gtaccccata
      241 tttcgacctt atgaccttaa tccttactga cttagattca tatccggttt ggtcatctct
      301 cccggaatat gttctacaat tggcagatct gttctggtat cggccactta aagaaacagg
      361 cgaacgttat cacagtatgg atattgaaga tgagttcggt ctatttaggt ctcatcacga
      421 ctattatcca gaaagtccat atcagactcc tatatattgg ttactacaat cacagttcaa
      481 aaaaacaata gactttattc ttgattttac gaacaagaca acgatatgtt ttgcccactc
      541 ccattttgct aaaaacgaaa ttgaagaagt cgatgtcttt attgaagaag gaaagtttat
      601 aaagcaatat atatgcaatc gtctgtggtg ctcatatcga ggaacacagg tctctaccta
      661 cttactttca tcaattcata tggcattgga aaaatttttt cttgagaatt ttaaaaatgc
      721 agactcgaaa gtgttggaaa gttggcttct tttcttgtta agaaatacca agtcagcttc
      781 tatttctgca gtagttacga gtattgtact tgcattccct gagaagacat tcaatgtagc
      841 taaagtacta ttccaaacaa aggatttctt ccgttttgat atgaatcgaa tggttctaga
      901 cagaacacat aaaagttcat taatctccct cagggatggc tttggcggta cagattacag
      961 aaactctttg cacgaagaag atagaattaa agcttgcgat gatgtgcata gaaatactta
     1021 tcttgaaaat cttgccttgc attatcaaat tttcaggagt gaaaatgtaa cggagaaaga
     1081 tgtcattgaa aggcaacaag tgctctgggg tattttcgac aaatactata atcagcttcc
     1141 agatgaagct caagaaactg aagccgataa gacgtggagg ctctgcttgg caagaatgga
     1201 tcggcgaaag atgaaaataa ctaccaagga gaaagatgaa gggattgaga tatcattcaa
     1261 tcctgagatt gaccctaaac taaagcaata tagtgaggaa gcaataaaga aaaactccga
     1321 gcatatgaag tatgtaacgc tgaaactatg ggcaagctat aaaagagaaa aggatgaacg
     1381 ttataagaat tatggaatgt atgaggacaa tccgcaaatt gctttacaag agaccaaaga
     1441 aataataaaa aagcttaatg aggaaggggg tgaagatttc agactattaa atggtaatat
     1501 accagcagac gtttgttctg tattactgtt agattacttt aatcagttga ataatgaaga
     1561 gagagaatat tgtaaagata ttgttctagc atattctcaa cttccgttga aggaaggcta
     1621 taattatcag gtacaagatg ggacaacctc ggcaatttca gccttacccg tgatttatca
     1681 taattatcca atggaaaggg agactataaa aacaatatta cttttgacac tgtttaatga
     1741 ccactctatt ggaatggcag gtgggcgcta ctcagtattt cctagtatgg tg
//
LOCUS       DAPHJE010000269         1786 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPHJE010000269 DAPHJE010000000
VERSION     DAPHJE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.272"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(100..1284)
                     /locus_tag="RH222_004531"
     CDS             complement(100..1284)
                     /locus_tag="RH222_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3799429.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1584..1678)
                     /locus_tag="RH222_004532"
     tRNA            complement(1584..1678)
                     /locus_tag="RH222_004532"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1642..1644),aa:Sec,seq:tca)
BASE COUNT          472 a          400 c          395 g          519 t
ORIGIN      
        1 acgcaaaaaa aataatataa taacaaaaat cctccagcct tgtaggccag aggataaaat
       61 acttaattga ggaaaatatc agggaaaagt atttctgatt taactttccc ggctggagag
      121 tgaatcaatg taatcagcgt accattgcat catttcacgg cggccatcga gatacagggc
      181 atgattatag gtacccctga tggaattttt atccacatga gctaactgca tttcaatcca
      241 ggcggaatta aagccttgtt catgcagaat tgtactcatg gtgtgtctga aaccgtgccc
      301 tgtgagtctt cccttatatc caagtaattc aattacttgg ttaatgcttt ccttactaat
      361 tggtttgcgg ggatcatttc taccgataaa gactagggga tgatgttttg agattggttc
      421 aagctgctta aatagcataa tgacctgctc agagagcggc acaatatgag gtcgcttcat
      481 cttcattact tctgccggga tctcccacaa ttttgtctca aaatcgatat cttcccagcg
      541 tgcaaaacgc agttcttgtg ttcgtacgcc ggttaacata atgatctgag tagctgtctt
      601 agtgattata cttccggtat aacccgccag atcgttgagg aagtggggaa gttcattagc
      661 agtaagaaag ggaaaatgta cttttttcgg tgtagctaaa gcactggcaa gatcgggggc
      721 tgggttatag tctgcccggc cagtaacaat tgcatagcgg aacacctcac cacagcgctg
      781 gcggaccttg cgcattttct ccagagctcc acgtttttcc attttacgta gtgcttccag
      841 taactccatt ggtttgattt cggcaatagg gcgcttgcca atgtagggaa aaatgtcttt
      901 ttcaaaggta tcaatgattt catcacgata gcgtaatgac caccgatcag cttttgactg
      961 gtgccattcg cgagcaatag cctcgaacgt atttttcaga ctcatttgct gcgcgatttt
     1021 ttcttccttt tttgcctccc ctggatcgcc tccagcagcg aggagtttcc tggcttcact
     1081 gcgtttctcg cgagcatcgg ctaacgttac atcggggtat acgccaatag aaagcatctt
     1141 ttctttacca gcaaagcggt acttcatgcg ccagtattta gaaccattag tgttaatcag
     1201 tagatacata ccgccgccat cagcaagctt atagggtttg tctttgggtt ttgcggtttt
     1261 cactttgata tcggttaatg ccatgacatc ctccggcttt tgatgaattc atcttcctga
     1321 gggggatttc tgccagtaac taacctgata cccccaatac accccttgta taacaaagat
     1381 attgttaaaa gccgttgtta tgtatcatat ttttcctatt atgggggtgc ttgtatttca
     1441 ataggaaata acacccccta aagtaccccc aaaagaaact tgatatgggg tgaagtaggt
     1501 tgacctcagg atacaaataa atactgatat acaagggatt agaagagatt tattgacatt
     1561 gggagagttc agaagaggta aactggcgga agatcacagg agtcgaacct gcccgggacc
     1621 gctggcggcc ccaactggat ttgaagtcca gccgcctcac cggagacgac gatcttccgc
     1681 gcctcgattg ctacatggag gcggggcgca ttatagctac ttccttgagt ttctacatcc
     1741 cccagatcag ttttttccgc ctaatacacg cagcgtatta tcacga
//
LOCUS       DAPHJE010000270         1781 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPHJE010000270 DAPHJE010000000
VERSION     DAPHJE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.273"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..342
                     /locus_tag="RH222_004533"
     CDS             <1..342
                     /locus_tag="RH222_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3799430.1"
                     /translation="ILAAPMALALTLLFMKQGHSDAAGIALSLLSGAVASAGAYVLWY
                     TLLPSLGSITASILQLSVPCLAMLGGVVFLGESLTVRTIFSALAVLTGIVMVTREKSP
                     RQPMAKARKPQ"
     gene            473..1696
                     /locus_tag="RH222_004534"
     CDS             473..1696
                     /locus_tag="RH222_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3799431.1"
                     /translation="MKYDFDEYVSREKTDSLTVDGWREYLFAGQSEFAMRYPAEGLIN
                     LWVADMAFATPEPILQAIRDRLDRKILGYTKIYDEEYYEIFGNWCERQYGHRFKTEDI
                     VLSPGIIPALNRLVPLLTDKDDSILIMTPSYGPFKKAGEYSQRRVVYSALKKSAGGWE
                     PDWEDFTRKASQADLRVKILFLCNPHNPTGRVWRPDELKRIIEICLANDIWVISDEIH
                     CDLSRSGIGHTPAASLFPDSTRIITCMSSSKTFNLAGNLLANIIIPDPGVRSEWRLRH
                     DEFISPLSLAANKAAWSECDDWLVHLREYIDGNFQYLHDYLISYLPETRFSIPEGTYL
                     AWIDISHYLPDTVGEDLSLYFARESGVLLEGGLLFVDNGEGHIRLNLACPRTMLTAGM
                     KRISQVLRTPAPNRV"
BASE COUNT          430 a          445 c          456 g          450 t
ORIGIN      
        1 attctcgcag cgccgatggc tctcgctctt acgttgctgt ttatgaagca gggtcactct
       61 gatgctgcag gcattgcttt gtcattgctg tccggtgccg tcgcctcagc cggtgcctat
      121 gtattgtggt ataccctttt accctccctc ggctctatta ccgccagtat ccttcagctc
      181 agcgttccct gcctggcaat gctagggggt gtggtattcc tgggtgaatc actgacggtc
      241 agaacgatat tttctgccct ggcagtgctg actggcattg tgatggtgac acgcgagaaa
      301 tccccccgtc agccgatggc aaaggcgaga aaacctcaat aacgcaatta attcatgcag
      361 ttgtaatgat tctcttatgt taatcacacg agccagtttt cccgctccgg catcagactg
      421 acaggaatac tgaggtcgac gtttcagact acacctaagg caggttcatg aaatgaaata
      481 tgattttgat gagtacgtaa gcagagagaa aacagacagt cttaccgtgg acggctggcg
      541 ggaatacctg tttgcggggc agtccgaatt tgcaatgcgg tatccggcag aagggctaat
      601 caacctctgg gttgctgaca tggcttttgc cacaccagag ccgattcttc aggccatcag
      661 agaccgtctg gatagaaaaa tactcggcta cactaaaatc tacgacgaag aatactatga
      721 aatctttggc aactggtgtg agcgacaata tggtcaccgt ttcaaaacgg aagacattgt
      781 actgtctccg ggcattattc ccgcgctgaa ccgtctcgtc ccgcttctga ccgacaagga
      841 tgacagtatt cttattatga cgccttcata tggcccgttt aaaaaggcgg gtgaatacag
      901 tcagcgtcgc gtggtgtact cggcgttaaa aaaatcagca ggtggctggg aacctgactg
      961 ggaagacttt acacgcaaag ccagtcaggc cgacttacgt gtgaaaattc ttttcctgtg
     1021 taatccgcat aacccgaccg gacgagtctg gcggcctgat gaacttaagc ggattattga
     1081 aatttgcctg gccaatgata tctgggtgat atctgacgaa attcactgtg acctgtcccg
     1141 aagtggtatc ggtcatacac cggccgcctc tcttttccct gattctacac gcattattac
     1201 ctgcatgtcc tcgagtaaaa cattcaatct ggccgggaat ttgttggcaa atatcattat
     1261 tccggatccc ggtgtcagaa gcgagtggcg tctcagacac gacgagttca tctctccgct
     1321 gagcctggca gcaaacaaag cggcgtggag tgaatgcgat gactggcttg tccatctgag
     1381 ggaatatatt gacggcaatt tccagtacct gcacgactac ctcataagct atttacccga
     1441 aaccaggttc tcgatccccg aagggacata tctggcctgg atagatatta gccactacct
     1501 gcccgacact gtgggtgagg atttatcgct gtattttgcc cgggagtcag gggtgcttct
     1561 tgaagggggc ttgctgtttg tcgacaatgg ggaaggacat atccggttaa atctggcgtg
     1621 cccacggact atgctgacag cagggatgaa aagaatttca caggtactgc ggacaccggc
     1681 ccctaacagg gtataacccc tttctctctg gccgtttgtc ctgtcaggat gtcgtcagaa
     1741 cccggcataa caccctgtgc cgggaacaaa aaaggcacgg a
//
LOCUS       DAPHJE010000271         1778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPHJE010000271 DAPHJE010000000
VERSION     DAPHJE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.274"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1061
                     /locus_tag="RH222_004535"
     CDS             <1..1061
                     /locus_tag="RH222_004535"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDV3799432.1"
                     /translation="PVFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVALTD
                     SAHTPYQVARFVEKPDRERAGAYLASGEYYWNSGMFMFRAKKYLSELAKFRPDILEAC
                     QAAVNAADNGSDFISIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSWSAL
                     WEVSPKDGQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMNRER
                     SQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFSMQM
                     HHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEIQSG
                     SYLGEDDIIRIKDQYGRC"
     gene            1085..>1778
                     /gene="rfbK2"
                     /locus_tag="RH222_004536"
     CDS             1085..>1778
                     /gene="rfbK2"
                     /locus_tag="RH222_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK2"
                     /protein_id="HDV3799433.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGSLRQISVLKEYVDHLM
                     GYVDLANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGAPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTA"
BASE COUNT          367 a          529 c          569 g          313 t
ORIGIN      
        1 agccggtctt ccacgacgcc atccgcgttg ccgagcagta tgccgatgaa ggccatctgg
       61 tcaccttcgg tatcgtgccg aacgccccgg aaaccggcta cggttacatc cagcgcggcg
      121 tggcgctcac cgacagcgcc cacaccccgt accaggtggc ccgcttcgtg gagaagccgg
      181 accgcgagcg cgccggggcc tacctcgcct ccggggagta ctactggaac agcggcatgt
      241 ttatgttccg cgcaaaaaaa tacctctctg agctggccaa attccgcccg gatatcctcg
      301 aagcctgcca ggccgcagtg aatgccgccg acaacggtag cgacttcatc agcattccgc
      361 atgacatttt ctgcgagtgc ccggacgagt ccgtggacta cgcggtgatg gagaaaaccg
      421 ctgatgcggt ggtggtcggt ctcgatgctg actggagcga cgttggctcc tggtccgcac
      481 tatgggaggt cagcccgaaa gacgggcagg gtaatgtcct cagcggtgac gcgtgggtgc
      541 acaacagcga aaactgctac atcaacagcg acgagaagct ggtagcggcc atcggtgtgg
      601 agaacctggt gattgtcagc accaaggacg ccgtgctggt gatgaaccgc gagcgttccc
      661 aggacgtgaa gaaggcggtc gagttcctca agcagaacca gcgcagcgaa tacaagcgcc
      721 accgcgagat ttaccgtccc tggggccgct gcgacgtggt ggtccagacc ccgcgcttca
      781 acgtcaaccg catcacggtg aaaccaggcg gtgccttctc gatgcagatg caccaccatc
      841 gcgccgagca ctgggttatt ctcgccggca ccggtcaggt gacggtcaac ggcaagcagt
      901 tcctgttgtc cgagaaccag tccaccttta ttccgattgg cgccgagcac tgcctggaaa
      961 accctggctg tattccgctg gaagtgctgg agatccagtc ggggtcgtac ctcggcgagg
     1021 acgacattat tcgtattaaa gaccagtatg gtcgttgcta atttttttcg ggacaaaacg
     1081 cagaatgaca cagttaacat gctttaaggc ttatgacatc cgtggtgaac tgggcgagga
     1141 gctgaacgag gacatcgcct accgtatcgg ccgcgcctac ggcgaatttc tgaaacccgg
     1201 gaagatagtg gtggggggcg atgtgcgcct caccagcgag tcgctgaagc tggcgttggc
     1261 ccgcgggctg atggacgccg gcaccgacgt gctggatatc ggcctgagcg gcacggaaga
     1321 gatttacttc gccaccttcc acctcggggt ggacggcggc atcgaggtga cggcgagcca
     1381 taacccgatg aactacaacg gcatgaagct ggtgcgcgag aacgcgaagc ccatcagcgg
     1441 cgacaccggc ctgcgggata tccagcgcct ggcggaggag aaccagttcc cgccagtgga
     1501 cccggcgcgt cgcgggagcc tgcgccaaat ttcggtgctg aaggagtacg ttgaccacct
     1561 gatgggctac gtggacctgg cgaacttcac ccgtccgctg aagctggtgg tgaactccgg
     1621 caacggggcg gcggggcacg tgattgatga ggtggagaaa cgcttcgcgg cggccggggc
     1681 gccggtgacc tttatcaagg tgcatcacca gccggacggc catttcccga acggtatccc
     1741 gaacccgctg ctgccggagt gccgccagga caccgccg
//
LOCUS       DAPHJE010000272         1704 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPHJE010000272 DAPHJE010000000
VERSION     DAPHJE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.275"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1374
                     /locus_tag="RH222_004537"
     CDS             <1..1374
                     /locus_tag="RH222_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV3799434.1"
                     /translation="AASALGAVSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTA
                     VDKLLMKPLYQQEMQLYSRYKSELAVWKNKEELLKAQKKALLSKLNKELRKGADESET
                     LRQLEVLQKNSAEEPVRYKFIFNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLS
                     ELPFINKMWDGSVLSVDRKNEPEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGF
                     LARCLISKPATTQGKRFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSP
                     EAQKIFIEHYNVLEQDLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADI
                     MTSAVVISSWYTDEYKKLFALPDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQ
                     CGPGRFRNRKKLNALLNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYH
                     YNKLRAK"
BASE COUNT          475 a          358 c          429 g          442 t
ORIGIN      
        1 gcagcgtctg ccctgggcgc tgtttcactt gtctgccaga atctgattga tgtctgcaga
       61 ctgaatacac tgcgggggcc ggtctctctt tttttcctga cgcttgccga gtccggagaa
      121 cgaaaaaccg cagtggacaa attattaatg aagcccctgt accagcagga gatgcagtta
      181 tattccagat acaaaagtga actggctgtc tggaaaaaca aggaagagct tttaaaggct
      241 cagaaaaaag cattgttgtc aaaactgaat aaagaactcc gcaaaggcgc ggatgagtca
      301 gaaaccctca gacaactgga agtacttcag aaaaacagtg cggaagaacc tgtgaggtat
      361 aaattcattt ttaatgatgc cacgactgcg gcaattaaga atcagctttg tggtaaatgg
      421 cgttcagtgg gtattatgtc agatgaagcc ggtattatat ttgatggata taccctgtct
      481 gagttgcctt ttattaacaa aatgtgggat ggctctgtat tgtctgtgga cagaaagaat
      541 gaacccgaac agatgattga aaatgccaga atgacgttat ccctcatggt gcaacccggt
      601 ctttttgacc gatatatgga gcgaaaaggt tctgttgcca gggacagtgg ttttcttgcc
      661 cgttgtctta tcagtaaacc tgcaaccacg caggggaaac gctttattaa cggagctgtg
      721 attccgggag gatcattaac ggcatttcat gaacgattaa tggaactggc cagaggcagt
      781 attgaaaaat ccagtgagga tgaacggtat tgtctgcatt tttcgcctga agcgcaaaaa
      841 atatttattg agcactataa tgttctggag caggatttat ccccttccgg acccttaagc
      901 ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg caagaattgc agcgttattt
      961 cagtatttca gctatggtga agggaaaatc tctgccgata tcatgacgtc tgctgtcgta
     1021 ataagttcgt ggtacacaga tgaatataaa aaattatttg cgttacctga tgaatctgaa
     1081 ctgcagcaga aggacgccga ggagctgttc gactggctca ttgaggagtg ccgcggagaa
     1141 tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg gttcagaaac
     1201 aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc agttcaggct ttctgtcgtg
     1261 ccggaaggaa aaaccatgta tgttctgctt ccccagatag ccagtttaaa gttgtctgat
     1321 gtctctggta tttttacctc gggttatcac tacaataagc tgagagcgaa gtaaccggtg
     1381 agcagttttt ctccattatg cttcagactt agatgattga ttattatggg gatttcatca
     1441 ccgatattca gatgtatgaa tatcggtgat gaactaaagc tcaggagctg cgtggtggtg
     1501 atgaatcaac aggcatataa ctgatacccg ccggtcagaa gttccagcct gcgactttcc
     1561 tgtctgcgtg cccgcagtcc ggcgttcagg tgcagcgtgt gacagaacgc acggagagcg
     1621 aaacggcagg cagacaggcc gttcagtcag ccctggcacc ctgcccggat accagcttcc
     1681 ctgaaacaac tggcatcagg tgcg
//
LOCUS       DAPHJE010000273         1674 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPHJE010000273 DAPHJE010000000
VERSION     DAPHJE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.276"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(394..606)
                     /locus_tag="RH222_004538"
                     /pseudo
     CDS             complement(394..606)
                     /locus_tag="RH222_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(962..1390)
                     /locus_tag="RH222_004539"
                     /pseudo
     CDS             complement(962..1390)
                     /locus_tag="RH222_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          456 a          362 c          385 g          471 t
ORIGIN      
        1 ttggattagt aatgcaataa agaaccatga tatcgattta tcacaagata aatcagggaa
       61 tacactattt tacttggaac gatatatgag aagatatcta ttcaaaaatc atgacaaagt
      121 tagacggaca ccaaacctaa aagcacggtc aatgatggtt ctggattttc tagtcgagca
      181 atattccatt acaggttacc tccttagaga agatatagcc tagcgaaaat gcttgtcact
      241 gtttatctgc atccatgaac tggatgcaga ttagtaagac tgcagagacc acaatgcctg
      301 aattgaccct acccacgtag tatggacaca ttcctaagcg aggttatggt tttcaaattg
      361 ttccgaactg agaccgccac accaactgtg ccgtcaccag cgattgtaat cgcactcgat
      421 ataattaaac accgttgcct gcattatttc ccgactgata aagtgttctc cgtggagaca
      481 ttccactttc agtgaatgaa agaagctttc cacgcaggga ttatcgtagc agcagccctt
      541 tgcgctcatc ctgccacgca gattatgccg ctttagcagc gccggataat ctgccgaaca
      601 gtacttcaat acccattttg ttcagggatt ttgctttagt ttgcgacata acacactgta
      661 tgaacgcatc cccgtttgca aggcttccgt tgttctgaca tcgacaagta gttgcagctc
      721 tgtatccggc cttattgcag tcagagctgc ggaccgctat gagattcgct gacctgatcc
      781 ttattcctcc aacggacttt atgtcctggc tgttattcag gtttctcagg taacggtctt
      841 accgtctttg tcatcactcg ggctgccatc gcaacctttc tgcattcacc ctttttaaga
      901 gttaatgttt atcggtatcg gcgattaatt tagcctgata ctgacgtatt ctttatcgta
      961 gtttgacgat gccaagtggc gtagcaacgg aggacgaata gggcgcggca gttagtgctg
     1021 tcgatgtcgg ttgcaggggc atcaacgagg ctgcttttcg ccgttcagca agcgccaaat
     1081 gttgatagtg ctgattctgc gtgatgtcca cacgccccat cagcatcgcc tgcaatttgc
     1141 cagagacttc cgcaatggca agaaaggtat tgatgttgtg ccggggaatg tgggtacgca
     1201 tccgggtggg agtgccatca tcttccagta attgatattt gctgaatact gatccatcag
     1261 gggcatgatt gcctaaaaat ttgttcatta gcacgttatt gaatgggatg ggaatagcca
     1321 gtaatgccag cgtgcgtttc aacgccaatg aaccttttgg acgcaaaaat aacaggtctt
     1381 catagttaat atcctcataa acggtgtgcg tatgaagaaa ttaaccagca aacataacgt
     1441 cctgcaagaa gaagatggag ggttgggtaa gtgtatgatt aatcgtagtc tgcgggtggt
     1501 tatgaactaa atggacgacg gactagattt aacttatgtt gagtaaaaac tagagagcaa
     1561 agagtcgtat ggctgttttc atcgtactaa taaagactga ctggttttgc cagaagggag
     1621 ataagctcag tccgtagtag ccagctaact gacaaattaa tttgtcggtc cctt
//
LOCUS       DAPHJE010000274         1673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPHJE010000274 DAPHJE010000000
VERSION     DAPHJE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.277"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="RH222_004540"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="RH222_004540"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV3799435.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPQVLHGASGLSTKDIQQ
                     TIKMGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..>1673
                     /locus_tag="RH222_004541"
     CDS             879..>1673
                     /locus_tag="RH222_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDV3799436.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRT"
BASE COUNT          420 a          413 c          459 g          381 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacattcact tatgctggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgctc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcatgggat
      541 gtatgccagc gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgcag gtgctgcatg gcgcatcagg gttatcgact aaggatattc agcaaaccat
      661 caaaatgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccctga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc ggcggcgatc
     1141 atctggggcc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa ggaatatgtt cgtgccggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgcggg ggatcccata ccgttagcac cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
     1381 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
     1441 cccatgttga agatgccgcc aatactttac gtacgcatca aaaggccttt attgcccgtg
     1501 ggctgacaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggc gtggaatttg
     1561 atcacagcaa tattatccat tatcagccgc aggaagcgca gccgctggcg caatggatag
     1621 aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg acg
//
LOCUS       DAPHJE010000275         1668 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPHJE010000275 DAPHJE010000000
VERSION     DAPHJE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.278"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..164
                     /locus_tag="RH222_004542"
     CDS             57..164
                     /locus_tag="RH222_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799437.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            370..1653
                     /gene="gltA"
                     /locus_tag="RH222_004543"
     CDS             370..1653
                     /gene="gltA"
                     /locus_tag="RH222_004543"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV3799438.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          436 a          405 c          393 g          434 t
ORIGIN      
        1 ctaatttgac aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc
       61 gtaaaagtta tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat
      121 tgaccaattc attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa
      181 gtacttgatt cgccatttat tcgtcatcaa tggatccttt acctgcaagc gcccagagct
      241 ctgtacccag gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc
      301 caggttgatg tgcgaaggca aatttaagtt ccggcagtct tacgtaataa ggcgctaagg
      361 agaccttaaa tggctgatac aaaagcaaaa ctcaccctca acggggacac agctgttgaa
      421 ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca
      481 aaaggtgtgt tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt
      541 acttttattg atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg
      601 gcgaccgatt ctaactacct ggaagtttgt tacatcctgc tgaatggtga aaaaccgact
      661 caggaacagt atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag
      721 attacccgtc tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt
      781 attaccggcg cgctggcggc gttctatcac gactcgctgg atgtaaacaa tcctcgtcat
      841 cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac
      901 aagtattcca ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac
      961 ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa
     1021 cgtgctatgg accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc
     1081 accgtgcgta ccgctggctc ttcgggtgcg aacccgtttg cctgtatcgc agcaggtatt
     1141 gcttcactgt ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa
     1201 gaaattagct ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat
     1261 tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc
     1321 gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg
     1381 gaagtggcta tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa
     1441 ctgtacccga acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct
     1501 tccatgttca ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc
     1561 gaaatgcaca gtgacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa
     1621 aaacgcgact ttaaaagcga tatcaagcgt taatggttga ttgctaag
//
LOCUS       DAPHJE010000276         1658 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPHJE010000276 DAPHJE010000000
VERSION     DAPHJE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.279"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..633
                     /locus_tag="RH222_004544"
     CDS             <1..633
                     /locus_tag="RH222_004544"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV3799439.1"
                     /translation="NKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDD
                     FYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYT
                     FSASQSYDENNKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGI
                     TGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNA"
     gene            808..1182
                     /locus_tag="RH222_004545"
     CDS             808..1182
                     /locus_tag="RH222_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799440.1"
                     /translation="MIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTD
                     QRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDE
                     QNLFCTITFQHVIDENKTYICQ"
     gene            1195..1658
                     /locus_tag="RH222_004546"
                     /pseudo
     CDS             1195..1658
                     /locus_tag="RH222_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          531 a          322 c          315 g          490 t
ORIGIN      
        1 aataaatatc tagtacaaac agccactcga tttagcgtgg ccgcctggcg ttatgcttcg
       61 caggattaca ggacattcag cgaccatctt tacgaaaatg ataaacacta tcatcagagt
      121 gattatgatg atttttatga tattggcaga aaaaatagcc tttctgctaa tattatgcaa
      181 cctttatcca ataatctggg aaatgtatca ttaagtgcgc tttggcggaa ttactggggg
      241 cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca acacattagt
      301 tatactttct ctgccagcca atcttatgat gaaaataata aagaagagga gcgttttaat
      361 ctgtttatct ccattccttt ctactggggg gatgatattg ccaaaacacg tcaccaaatt
      421 aacttatcga attcgacctc attttccaaa gatggctatt cctccaacaa tactggaatt
      481 actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa tcagcaacaa
      541 caaaataatg atacctcgct tggtacgaat ttaagctgga gaactcccat cgccataata
      601 gatggcagct atagtcattc taaaaacgcc tagcaaagtg gtggaagtat tagtagtgga
      661 ttagttgtct ggcccggtgg tattaatatc actaaccagt tatccgatac atttgcaatt
      721 ctggatgcgc ctgggttaga aggtgcacat attaatggac aaaaatacaa tcgaacaaac
      781 agcaaaggcc aggttgttta cgacctgata atacctcatc gtgaaaacca tctggtactt
      841 gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat tattgcacct
      901 taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc ctggtatata
      961 caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact ggatctccag
     1021 gaaaataata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt agatgaaatt
     1081 cccactggaa taaaagttgc tctcaatgat gaacagaact tattctgtac tattactttt
     1141 caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt tactatgaag
     1201 tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc tccctgtgca
     1261 ttgaccaatg tcggtgaaca acgtggaaca tatatactca agccactgag tatgaaaggt
     1321 aatctaaccg ccgaaaaatt gaaaacaccg gaaatatcat tgagtgtaca gacttgcaag
     1381 agacaaccaa cgccattaaa gttcattttt taacacagaa cgctttaagg tttaaagtcg
     1441 gaagtcgtca ttatattatt aatttcccct ttgaatctga cggtaaaaaa atagagcttc
     1501 cagggaagtc atcttataat ttagaagaaa tgctatctgc gataaatttc accattccat
     1561 attctatcgc atctgtatca gatagttcta acaccgcaga catttttcct ggaactcctt
     1621 ttcctttgtt gttcgaaatt gaagtgagtt catgtaca
//
LOCUS       DAPHJE010000277         1626 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPHJE010000277 DAPHJE010000000
VERSION     DAPHJE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.280"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(96..1502)
                     /gene="gndA"
                     /locus_tag="RH222_004547"
     CDS             complement(96..1502)
                     /gene="gndA"
                     /locus_tag="RH222_004547"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV3799441.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVQEFVESLETPRRILLMVKAGAGTDSAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILKQIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYALLKGGLALSNEELAQT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAQNAGIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAIAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLE"
BASE COUNT          430 a          411 c          384 g          401 t
ORIGIN      
        1 gaagacgtac aaataagatg actttgatca gtttattgta ggaaaaaaca atagataaca
       61 gtcaggccaa ttggcctgac tgtggtatat aaagattatt ccaaccactc ggtatggaat
      121 acaccttctt tatcagtacg tttataggtg tgcgcaccaa agtaatcacg ctgagcctgg
      181 atcaggttag ctggcagaac tgcggaacgg tagctgtcgt agtaagcaat cgcggctgag
      241 aacgtcggta ccggaatacc attctgcacc gcatacgcca cgacatcacg cagcgcttgc
      301 tgatagtcat cagcgatctg tttgaagtat ggcgccagca ggaggttagc aataccagcg
      361 ttttgcgcat aagcatcggt gatcttttgc aggaactgcg cacgaataat gcagccagcg
      421 cggaaaatct tagcgatctc gccgtagttc agatcccagt tgtattcatc agaagcagca
      481 cgcagctggg agaagccctg agcataggaa acgattttac cgaggtacaa cgcgcgacgc
      541 actttttcga taaattcagc tttatcaccc gccggctgag cctgcggacc actcagcact
      601 ttcgaagcag ctacacgctg gtcttttagc gaagagatat aacgagcaaa tacggactcg
      661 gtgatcagag acagaggttc gccgagatcc agtgagctct ggctggtcca cttaccggta
      721 cctttgtttg ctgcttcatc aaggatgaca tcgacgaggt atttaccctc ttcatccttt
      781 ttagtgaaga tatctttggt aatatcaatc aggtagctgc tcagctcgcc ttcgttccat
      841 tcagtgaagg tttgtgccaa ctcttcattg gagagcgcca gaccgccttt caacagcgca
      901 taagcttcag caatcagttg catatcgcca tactcgatgc cgttatgaac catcttcaca
      961 taatgaccag cgccatcagc accgatatag gtgacacaag gctctccgtc ttcagcaacc
     1021 gccgcgatct gtttcagaat cggagcaacc agctcatagg cttctttttg gccacctggc
     1081 ataatggaag gccctttcag agccccctct tcaccaccgg aaacaccggt accgataaag
     1141 ttaaaacctt cggcagacag ttcacggtta cgacggatgg tgtcctggaa gaaggtgttg
     1201 ccgccatcaa tgatgatgtc gcctttatca aggtaaggct tcagggaatc tatggcgcta
     1261 tcggtgccag ccccggcctt caccatcaac aggatacgac gtggtgtttc gagggattca
     1321 acaaactctt gtactgtgta ataaggaacg agtttcttgc ctggattttc ggcaatcact
     1381 tcttcggtct tttcacggga gcggttgaat acggagacgg tataaccacg gctttcgatg
     1441 ttcagcgcaa ggttacgccc catcacagcc ataccgacaa ccccgatctg ttgcttggac
     1501 attacatact cctgtcaggt gtagtcactg cgacacattc acagcttaat gagacatcta
     1561 tcctaacgca aataaagtga ggcgcctagt ttgtaaaacc caaaaagttg cgcaaaatcg
     1621 aatagt
//
LOCUS       DAPHJE010000278         1617 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPHJE010000278 DAPHJE010000000
VERSION     DAPHJE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.281"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..282)
                     /locus_tag="RH222_004548"
     CDS             complement(<1..282)
                     /locus_tag="RH222_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HDV3799442.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEVHEPMVICNEEHRFLVAEQLRQLNKLSSNIILEPVGRNTAPAIALA"
     gene            complement(562..846)
                     /locus_tag="RH222_004549"
     CDS             complement(562..846)
                     /locus_tag="RH222_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799443.1"
                     /translation="MSRLSHLSEHSAAAVCKGPSSLNARGLPGGEDDVSSYLSPRRKR
                     SFLTVASIGWAPVALFSVAVRACVLPCVIEKVLMVNPAANAESVVRLAAR"
     gene            complement(1552..>1617)
                     /locus_tag="RH222_004550"
     CDS             complement(1552..>1617)
                     /locus_tag="RH222_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV3799444.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          449 a          413 c          380 g          375 t
ORIGIN      
        1 cgccagggcg atggccgggg cggtgttgcg cccgaccggc tcaagaataa tgttgctgga
       61 cagcttgttg agctggcgca gctgttcggc caccaggaag cggtgctctt cgttacagat
      121 gaccatcggt tcatggactt caaggcccga gagtcgggtg atagtttcct gcagcatgga
      181 gttctgcccg aacagacgga ggaactgttt cggataaagc tcgcgagaca tcggccagag
      241 acgactgccg gtaccgccag ccataattac aggaagtaac ataatgagat ccttaaataa
      301 ataattgctt tattttacta tcaatcaatc gaccgtcagg tggcgtggcc tttttgaggc
      361 acgctaccgc cattggctgt cgcaaccaaa atgcgtactg ctcccgtgct ccaagctaag
      421 cgccaccctc tttcgcttca ggctgtcggc agatcgttca gtcagcggca acaatgacag
      481 tctggcggaa cgcgtgtcct gaaaacctaa aagcagagat ataccttcag gcatatctct
      541 gcttttaaca tgtatcttcc ctcacctagc cgccaaacgc accacacttt ccgcatttgc
      601 cgccgggtta accatcagga ctttctcaat tacgcagggc aaaacgcatg cccttaccgc
      661 caccgaaaaa agcgcaacgg gcgcccagcc gatgctggca accgtcagaa aggagcgttt
      721 acgacgtggg gaaagataag aagagacatc gtcttcaccg ccaggaagac cgcgcgcgtt
      781 caggcttgat ggccctttgc acacggcggc ggcgctatgt tccgacaggt gggataaacg
      841 actcatgctc tacaatgcac attccacaaa gaaaatcgtc cgcagattat agcgctggac
      901 gccgggaata aaaataaaaa agcctactga ttaatagatt actcaaataa tacatatctt
      961 aaacattcat taaacaaaaa taagcctatg atatagaaga caaaacgaag gaaagcatgg
     1021 cgcgattttt gcccatttgc taaatgttcc gctgacgaaa aatataactt taagcaaaat
     1081 tacgccgtcc cggagggtat tggctgcctt tagggtatcg ccacgaatcg ctaaaccacc
     1141 gaccgctcac aaagttatta ttcggatgct aacagtcaaa tgagaaatgt tgttatttat
     1201 caccttttgc cacggttatt ttgccagaaa aaacgtcctt cacctcacgt ctggcggaca
     1261 tgacgcggca aagcaagtga tcccaaccgc aagccagtgt aaaatcggca cttatgtaga
     1321 acggactgaa cactcttgct cttaggggct gacaaactgg tacacctacg taatctcatc
     1381 gccacgagat gccgaccact atttgcacac tgcatccttt ggctacaact cactggttga
     1441 gatgatggac tttaaatcct ctctggaaga agctctagga ttgaagcaaa gaagaataag
     1501 atgcccatct agtcgcgtga tgtgctggac gccaggggta atgactccaa cttattgata
     1561 gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccaccg attttga
//
LOCUS       DAPHJE010000279         1610 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPHJE010000279 DAPHJE010000000
VERSION     DAPHJE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.282"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            78..293
                     /gene="essD"
                     /locus_tag="RH222_004551"
     CDS             78..293
                     /gene="essD"
                     /locus_tag="RH222_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV3799445.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            298..609
                     /locus_tag="RH222_004552"
     CDS             298..609
                     /locus_tag="RH222_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDV3799446.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            606..1139
                     /locus_tag="RH222_004553"
     CDS             606..1139
                     /locus_tag="RH222_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3799447.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1136..>1610
                     /locus_tag="RH222_004554"
     CDS             1136..>1610
                     /locus_tag="RH222_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDV3799448.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESL"
BASE COUNT          454 a          336 c          423 g          397 t
ORIGIN      
        1 tgtgctgtca aaactacccc gtagactccg atctttttca aacatattgc accatccgtg
       61 tacatcgggg tgaggatatg aaatcaatgg ataagttaac aacaggtgtt gcctatggca
      121 catcggcggg taatgctggt ttctgggcat tacagttact cgataaagta actccgtcac
      181 agtgggctgc aatcggtgtg ctgggtagcc tggtttttgg cctgctgacg tatctgacaa
      241 atctttattt caagattaaa gaagacaggc gtaaggctgc gagaggagag taatccaatg
      301 actcaagact atgaactggt tgtgaaagga gtccgtaatt ttgagaataa agttacggta
      361 actgtagcct tacaggacaa ggaacgcttt gacggtgaaa tttttgacct ggatgtcgcc
      421 atggaccgtg ttgaaggagc tgcgctggag ttttatgagg cagcagccag aaggagcgtc
      481 cggcaagtct tcctggaagt agcagaaaaa ttgtcagaaa aagttgagtc ttatctgcag
      541 catcagtact cctttaagat tgaaaatcct gccaataagc acgagcgtcc tcatcataaa
      601 tatatatgaa cacaaaaatc agatacggcc tgtcggctgc cgttctggcg ctgattggtg
      661 ctggcgcatc tgctcctcag atacttgacc agtttctgga cgaaaaagaa ggtaaccaca
      721 caatggcata ccgcgatggt tctggcatat ggaccatctg tcggggtgcc acagtggtgg
      781 atggaaaaac cgtttttccc aatatgaaac tgtcgaagga aaaatgcgac caggtcaacg
      841 ccattgagcg tgataaggcg ctggcatggg tggagcgcaa tattaaagta ccactgaccg
      901 aaccacaaaa agcgggtatc gcgtcatttt gtccctataa cattggcccc ggtaagtgtt
      961 tcccgtcgac gttttataag cggctgaatg ctggtgatcg taaaggtgca tgcgaagcga
     1021 ttcgctggtg gattaaggat ggcggacgcg attgccgcat tcgttcaaat aactgttacg
     1081 gtcaggttat tcgtcgtgac caggagagcg cattaacctg ctgggggata gaacagtgaa
     1141 tcagatattc atggtgattt ttctcgtgtt gtcaggattt atcgtcggaa atgtctggag
     1201 cgacagagga tggcaaaaaa aatgggcgga acgtgatgct gccgcattat cacaagaggt
     1261 aaatgctcaa tttgctgctc gaataattga acaggggcga actatagccc gtgatgaggc
     1321 tgttaaagat gcacaacaga aatctgctga aatttctgcc agggctgctt atctgtctga
     1381 tagtgttaac cagttgcgtg ccgaagcaaa aaaatatgcc atacgccttg acgcagcgaa
     1441 gcataccgca gatcttgccg ctgccgtcag aggcaaaaca accaaaaccg ccgaaggaat
     1501 gctcaccaac atgctcggag atattgcagc agaagctcag ctttatgctg aaattgctga
     1561 cgaacgctac atcgcaggag tgacttgtca acagatctat gaatctttaa
//
LOCUS       DAPHJE010000280         1592 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPHJE010000280 DAPHJE010000000
VERSION     DAPHJE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.283"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            298..1448
                     /locus_tag="RH222_004555"
     CDS             join(298..559,559..1448)
                     /locus_tag="RH222_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3799449.1"
                     /translation="MTQPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELAILPKGRDILREAPEMKY
                     VFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAK
                     QRYGAPRLADELPEFNIKTIAASLRRQGLRVKASRKFSPVSYRAHGLPVLENLLEQDF
                     SASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVNGWSMSPRMTAQLACDALQMAL
                     WRRRRPESVIVHTDRGGQYCSGDYQALLKRHNLRGSMSAKGYCYDNACVESFFHSLKV
                     ECIHGERFSSREIMRATVFNYIECDYNRWRRHSACGGLSPEQFENHNLA"
     gene            1480..1554
                     /locus_tag="RH222_004556"
                     /pseudo
     CDS             1480..1554
                     /locus_tag="RH222_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          406 a          390 c          436 g          360 t
ORIGIN      
        1 catggctatt acaatccaaa agtcttggta tagatcttaa attttttgtt tctccataag
       61 aaatttcgcg acagagatcc ttaacggtgc gtcgagcttc gacggaattc agaataatga
      121 tggtctggtg ttcggtgaat cgtgctttgc gcatggcgat ctcctatcag aacaaaacca
      181 gtatgccgga tgatctctaa aagtgaatgg accgatatgc agggatgctt acagtgatct
      241 tacccaccaa tagtggacac aggactaagt gagtaaactc tcaaccagag gtgactcatg
      301 acacaaccag tatcaatcag caagaagccc cgtaaacaac atacgcctga atttcgtaac
      361 gaagccctga aactcgctga acgcatcggt gtggccgccg cagcccgtga actcagcctg
      421 tatgaatctc agctttatgc ctggcgcagt aaacagcagc aacaaatgag ttcgtcagag
      481 cgcgaaagcg aactggccgc tgaaaatgtc cgccttaaac gacaactggc ggagcaggct
      541 gaggaactgg ccatcctcca aaaggccgcg acatacttcg cgaagcgcct gaaatgaagt
      601 atgtcttcat cgaaaatcat cgggcagagt tcagcatcaa agcgatgtgt cgtgtacttc
      661 gggttgcccg cagcggctgg tatgtctggc tcaggcgtcg tcaccagatg agcctgcgcc
      721 aacagtttcg gctcacctgc gatgccgctg tccataaggc attctttgag gcaaagcagc
      781 gatacggtgc tccccgcctt gctgacgaac tgccggagtt caatattaaa accattgccg
      841 ccagcctgcg tcgtcagggg ctgcgggtga aagccagccg gaagttcagc ccggtcagct
      901 accgtgcaca tggcctgccc gtattggaga atctgctgga gcaggacttc agtgccagcg
      961 gcccgaacca gaagtgggcg ggtgacatca cgtacttgcg taccgatgag ggctggttgt
     1021 atctcgcagt agtcatcgac ctgtggtcac gcgccgttaa tggctggtcg atgtcaccgc
     1081 gaatgacagc acaactggcc tgtgatgcac tgcaaatggc gttgtggcgg agaagacgcc
     1141 cggaaagcgt cattgttcat acggaccgtg gtggtcaata ctgttcaggg gattatcagg
     1201 cgctgctgaa gcgacacaac ctgcgtggca gtatgagtgc gaaaggctac tgttatgaca
     1261 atgcctgtgt ggaaagcttc tttcattcgc tgaaggtgga atgtatccac ggggaacgct
     1321 ttagcagccg ggaaataatg cgggcaacgg tgtttaatta tatcgagtgt gattacaatc
     1381 gctggcgtcg tcacagtgcc tgtggcggtc tcagcccgga acaatttgaa aaccataatc
     1441 tcgcttaggg ccgtgtccac attacgtggg taggatcaca gtgctattgc ccgggaattt
     1501 aataccaccc gccagactat ccttcgggta aaggctggat tgctgcaaga gtgagcggct
     1561 gggaagatta taaaaaaaat caaatggtta tt
//
LOCUS       DAPHJE010000281         1587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPHJE010000281 DAPHJE010000000
VERSION     DAPHJE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.284"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..384)
                     /locus_tag="RH222_004557"
     CDS             complement(<1..384)
                     /locus_tag="RH222_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3799450.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYIFDHNAKPDIWDYFKAMGVGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGL"
     gene            complement(393..1160)
                     /gene="sctT"
                     /locus_tag="RH222_004558"
     CDS             complement(393..1160)
                     /gene="sctT"
                     /locus_tag="RH222_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV3799451.1"
                     /translation="MEEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1162..1422)
                     /locus_tag="RH222_004559"
     CDS             complement(1162..1422)
                     /locus_tag="RH222_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV3799452.1"
                     /translation="MNDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1432..>1587)
                     /locus_tag="RH222_004560"
     CDS             complement(1432..>1587)
                     /locus_tag="RH222_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV3799453.1"
                     /translation="ISSVLLTLGMMMMSPVIISTPIKLILFVAMDGWTMLSKGLILQY
                     FDLSINP"
BASE COUNT          550 a          317 c          278 g          442 t
ORIGIN      
        1 taagccaaat atacgcttta agccatttac tggattcaat gaatcaaact taaatttaac
       61 agcttcagtt gctaattgca ttttactttg aaaccaggag acaagtattg ttgtgaacat
      121 gcaaaccagc aaaaaaggaa tgatcgtttt caaccaacca acccccatag ctttaaaata
      181 gtcccaaata tctggtttag cgttatgatc aaagatgtat tcaagaatcg acataatgtg
      241 atgcacatca aaaacatatc cgagatataa agtacccaca agcattatta cagagatggt
      301 taagtctcta ctttttagaa tttgcccttt tttagaagca tcctggagtt tcttttgtgt
      361 tggcttttca gttttattgg ccatgaaaca ctctatttct caatgtatag ttgcaattgt
      421 tctgggaaaa aggataacgg cattactcgt tcagccagta ttgggccaaa ataaataatt
      481 aatattaaaa atgccagcgc acttttgaca gtcagtgaaa tggcaaaagc atttagttgt
      541 gaagcataac gtgccagcaa tcccaggaca gcctcaccgc ccagcataac ggcaatcacg
      601 gggcttgcgt aaacgatagt atgagtcatt atgttattaa tatatgagaa taaaggctct
      661 aatttaggaa aattaaaatt gcctgaaggc cataaattat aactttgcca aagagtttcc
      721 agaataaagt tcaatccacc gttagtaaga taaaccgctg cagagaatag gttaaataat
      781 cttgctaatt ccgaagtatc gacaccagta gcaggatcca aagtactact taacgttgca
      841 ccacgttggt tatcgataaa actacctata gcaaggaaaa tccaaaacgg ggatgataat
      901 aagcaagcaa tgaacagccc tatgataatt tctctggcag caaacaaagc gatatcgatt
      961 tcatttaagg tcgataattc atagtggtaa tgctgaaata atgcacatga taccactatt
     1021 attattggat gacggactac tgttggtata ttcccactgg cgaaaaatgg taacaaatag
     1081 aaagttggtg ccaatcggca aaaaccgagc gctgtagcag ccagcagact atgcaactga
     1141 tagagtatcg cctcctccat cctatcctct cgcgaatgct aaattcagca tttcgatacc
     1201 aaacgaatat aatttttcac cataccatcc gctcattaaa aaaaagcaaa tacttacgca
     1261 taataatttg acgccaaatg gcaacgtctg ttcttgtaac tgtgttaccg tttgaaaaag
     1321 ccctaccaat aaaccaacaa atgtcgcaac tgcgataggt cctgcagaca taactaaaat
     1381 cagatacagc gccctattcc ctgcaaaaac aatatcgttc atattttttc ctcagggatt
     1441 aatggatagg tcaaaatatt gtaaaatgag tccttttgat aacattgtcc aaccgtccat
     1501 agcgacaaat agaattaatt ttatcggtgt agatatgatt accgggctca tcatcatcat
     1561 acctaacgtt aacagaacgc tggatat
//
LOCUS       DAPHJE010000282         1559 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPHJE010000282 DAPHJE010000000
VERSION     DAPHJE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.285"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..947)
                     /locus_tag="RH222_004561"
     CDS             complement(<1..947)
                     /locus_tag="RH222_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3799454.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAK"
     gene            complement(973..>1559)
                     /gene="rfaY"
                     /locus_tag="RH222_004562"
     CDS             complement(973..>1559)
                     /gene="rfaY"
                     /locus_tag="RH222_004562"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDV3799455.1"
                     /translation="KVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
                     YYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPE
                     NVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAME
                     RHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          483 a          325 c          267 g          484 t
ORIGIN      
        1 ttctttgcat gttgatattt cacttgatac tgcttttcat tcgtggctgg aataaacgcc
       61 acatcattcc agcaagaagc ctgataagcc tcattaaaat acttctggca gggataattc
      121 gtccagtcgt tccagggctt cgttacgcca acataatgaa tcagcttagt atcatcggta
      181 atggtctttt tataatcttc atcgctttta ttttttaact catagtcaat gccataaaag
      241 caatcataat cgccgctaat aaaaatacaa tgaccaaaga acagaatatt taaaatatcc
      301 tgatcaaggc atttatactt cgtgtgctgc gggtcacttg ccagcatagc aatggcttta
      361 ttcataaaat catttttcgc ccatgcttca agctgcagat atactacacc ggcgttgaaa
      421 taattccctg gaagtccctc tatagccaga cgcttcgctg gtttttcctg cataaaaggc
      481 acatctttaa taacagctgc gaacttatcc tcatcaaaat taatatcaag caatccagct
      541 aatgagcctt tacagatgac atcagcatcg atatataaca gattttcaat agtttcactc
      601 aaatactgga acgcaactaa acggaagaat gttgcataag accactgttt caggctgggg
      661 aagatatcaa taaaggcatt ggagacttta taaattatta cttcaacgtt agcatttttg
      721 ctggtaaaag cattaacacg ttgtagataa tcttcatcaa tgtagtcggt aaagagatga
      781 aacttaaccg caaggtccgg gttatgcata acaactgatt gcatagatac tgccgcacca
      841 aataaaaagt tacggtcaat accgtaagcg atatttaaca cacgatcggt gttagcaatt
      901 tccccagaga cagcgacttc agtaatactc tcagctaata aatccatgtt ggttccgttt
      961 tgactgtgtg gtttacgctt tgccttttaa ttttttaata aatttgcgta gtttggttct
     1021 gtagattact gaatagtagc cataatcttt tatttcgttg gcaataccca ggtgacgttc
     1081 catggccagg cgatcacgag ctttacgctc tgccgtacag cttttaccgg ataggtcaat
     1141 tatcctgaca ccatctttac taacgataaa gttaccccgg tgtgggtcgc ccgatagcat
     1201 atttaaggca tgcaattttt ccattgaagc cttaatttct gctttaacat tttctggaat
     1261 aatcggcata tcattgagct ctacgccctc gacatactcg ataagcatga taaaaacact
     1321 ggcatagttg aaaattttac gctcagccag aaaataaaaa tcattaggaa aagtaagacc
     1381 tgcacttctg acacgatcgg tttcaacgat caaattctga tagtagtcac cttttacaaa
     1441 agattttaaa aaacgttcat tccttttcgt tttcggagca aagactttaa aaacatagcg
     1501 accatatttc gtatcaatca acgatacttt ggtatcatca atattacgta atactttta
//
LOCUS       DAPHJE010000283         1540 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPHJE010000283 DAPHJE010000000
VERSION     DAPHJE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.286"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            558..785
                     /locus_tag="RH222_004563"
     CDS             558..785
                     /locus_tag="RH222_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799456.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(820..960)
                     /gene="ykgO"
                     /locus_tag="RH222_004564"
     CDS             complement(820..960)
                     /gene="ykgO"
                     /locus_tag="RH222_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV3799457.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(960..1223)
                     /locus_tag="RH222_004565"
     CDS             complement(960..1223)
                     /locus_tag="RH222_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV3799458.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          447 a          297 c          345 g          451 t
ORIGIN      
        1 gcttttttga taagaagaaa aagacatttg gtttcataag gttattggca atataacgtt
       61 caattcttac ttgcatttta tattggtcag gaactttgcc ctgaagtgtt aggaatagtc
      121 ttaattcgct gtgtgatgga ctagtaaaaa aacaagtttt aagggtaagg gatagaatat
      181 aaaactatga tgagctgtca ttgaagatga tttgccaatc agtaagatat tttaatggat
      241 taacatgccg cacaaaatgg ttacaggtgt tcattccaat aagtaatgga aaattgaacg
      301 ggcgtatgta cgtcatctgt tcggtaagcc cccaagagcc agattaagtg aagttaaacg
      361 aaggctgcca gctgtcacag gaacgatggc ctttttgtca gggcactgca ttcagttatc
      421 tgtgcgcgtt actctaactg gcactcgaac gtcggcgacg gggttaccgg tggatcagac
      481 tgttcctgtg gcgtaaacgt ttttatgtta gtaccaacag atttaccaca tttatcatct
      541 taacagtttg agtgaaaatg caggctgcgc cgcaaaggga ggagactgag tggcgtgttc
      601 agtctaaaag agggttgatg ccagcataca ggggcgaagc tggtcaacaa gtcaacatta
      661 agataatgga atattcggag agaaacgtca ggcagttggc ctcaaatgaa caggaagaat
      721 atatccccag gaaaattaat gttggtgtta taaacacacc aacattaatc cgatctgatt
      781 attaatcaga caaatatcgt taatccgtcg aattttgaat caacgttttt tcttacgacc
      841 ctgaacggcc ttaaaacgtg gattagattt acaaatcaca tatagccgtc cttttcgctt
      901 cacaatctga cagtctggat ggcgttcttt tgcggtacgc agagagttaa gaactttcat
      961 cacgccccct ttttcgtgct aacaaaacga ccaaaacgtt gggtgaatcg tgcaacattt
     1021 ccttctgatg ccactgttct cagcttccct gtatagaacg ggtgcgattt agaagagaca
     1081 tcaattgtca cgtatggata cgttacgcca tccagctcaa tctcacggtc tgttttgata
     1141 gtcgagccga ttttaaagta ctcatcaaca ctggtgtcgt ggaacaccac agtacgatac
     1201 tcaggatgga tattgggctt catcattttt acctgttatg ttataacata accataaaga
     1261 tacatgctca tccggatcat ttcaacactc aatccctgtg actctttgtg gatgtggaat
     1321 tttcgcaacg gggaactctg gcattgttag cagccattgg tgacgctccc ataaacgcct
     1381 ggatgagtga ttgatgatgg ttttgctgag agtatggatt tttcgagaat ggaggttatc
     1441 tccactttac tggccgatga tttatcgcag tatgattgat tacctgacag cgtgtattcc
     1501 tctcccacgg atataataat tacttacaaa aaagggagag
//
LOCUS       DAPHJE010000284         1505 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPHJE010000284 DAPHJE010000000
VERSION     DAPHJE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.287"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..980)
                     /locus_tag="RH222_004566"
     CDS             complement(<1..980)
                     /locus_tag="RH222_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3799459.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKETRPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQF"
     gene            complement(1291..1395)
                     /locus_tag="RH222_004567"
     CDS             complement(1291..1395)
                     /locus_tag="RH222_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDV3799460.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT          450 a          335 c          332 g          388 t
ORIGIN      
        1 ataaactgtt catagccgac ccatgcggag tcaaagagga tgtagtcaca cagatgacca
       61 attttatcca ccacctgacg ggcgttataa atcgtaccgt cgtaagtgcc tagctgaata
      121 acggcgaggc ggaaaggacg agtctctttt gcccgctgcg gtgcaacttc tgtaatcagc
      181 tcacgcaggt aactttcttc aaaacagtgc gcatcaatgc caccgataaa gccatacggg
      241 ttacgtgcgg tttccagata aaccggtgtc gcgccagcct gtagcaacgc accgtggtgg
      301 ttagatttat ggttattgcg gtcaaacaac accagatcgc ccggcgtcag caatgcgttt
      361 aagaccactt tattagaaga tgaagtaccg tttaaaacga aataggtttt atcggcatta
      421 aagactttag ccgcatgctg ttgtgcaata catggcgcac cttcatgaat cagcagatcg
      481 cccattgcta cgtcagcatt acacaagtca gagcggaaga gcatttcgcc aaagtattcc
      541 acgaactgat tgcctgccgg atggcgacgg aaaaactcac ccccctgatg accagggcag
      601 tcaaaagcac tattgccctg attaacataa tcgaccagtg cgcggaagaa gggcgggcgc
      661 agttgcgttt cataatggct ggcagcggtt tctaactgac gtccataaaa ctctttacgt
      721 gattcgcaat gttcgaagac accagaaata cgtggcagat attctgcggg tacacgttct
      781 tcattttccg ttgcaataaa aacaggaatg tcatagccag tagcgtctat ttcatcgagt
      841 tttccacgtt caacatcact gactgataaa ataatggcgg caacgtcaat ataattactt
      901 tcgtctatat tcacgcatgc acgatgagtg gaaaaacaat ccgggcaaga acgactaacg
      961 gcaattttta attctgacat tttgttcatc tctttatttc aggtaatagc aggtcctcaa
     1021 tttctcaaag gagaaatcga tcacgccgga aaaaaagcaa agctccaccg ctgataaata
     1081 aatcagtagt ttgctttttg cagatgcaat aacccagacc agcccggtaa ctggctatac
     1141 tcgtcatacc tcaagttgca agtgctgcga ctcgaattat ttagagttta aaagggttag
     1201 cagtatatga gcaccgagga atacaggtat taacctgtaa tgcttaaagt cagggagacc
     1261 tgtacgttgg ggtgcccttt agtcagaaga ctagcgggca ttaaagaaat gaaagtcaaa
     1321 actattacga tgggcaaact tcatgatatg ggttgtccgc cttatatgag gcattaagcg
     1381 gttattattt tccatttacc tttacctctt gctgtgtcag ttgaaggtag agttattcta
     1441 tccatgaaat ggctataatc tgtgaagaag atcaacaaaa aacgatcata ccccaaataa
     1501 taaat
//
LOCUS       DAPHJE010000285         1460 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPHJE010000285 DAPHJE010000000
VERSION     DAPHJE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.288"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(278..553)
                     /locus_tag="RH222_004568"
     CDS             complement(278..553)
                     /locus_tag="RH222_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799461.1"
                     /translation="MKLINQLKINKSVNFYILIILITRCIACIFFMAMLGTILGRLIV
                     FSKTQVLFFDWRADIIYSLKVGGGAGMLAGIGIWIKAKLQERKDDKK"
     gene            complement(522..1130)
                     /locus_tag="RH222_004569"
     CDS             complement(522..1130)
                     /locus_tag="RH222_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV3799462.1"
                     /translation="MEKEYGTAVAQELVTGLLSGQDYIKRNPDTEAMAKAQSIMNTWG
                     YHKSNASIGDAPLLFGGSVLGLTVKGMATNAAIGISVNSAAQLAGKDPFSYVDAIIAG
                     VTAAATTGKGIIVSTPINMSGAAIGSSIKGEDPTNSVIGTGLGSVFGGGIGKVISGSM
                     GSTVKEGTSDIIGNIGGSITGEAIGNVTKGTLDEADKSIKNK"
BASE COUNT          412 a          372 c          219 g          457 t
ORIGIN      
        1 gcatcatggc tcatctgata atcacctatt atttatacgg gaaaagttga agatcatgct
       61 gtttattttc atctgtattg tcaccagtca gcatatgccc cggtccccga ttaggttgcg
      121 tgttttcatg gaaggagagc gcccaataac ctctattgag attggagtta ctcaccgatc
      181 acttccacgg taatacgcac attacctcat tcctgatggc attgaccaag aacataacta
      241 ccaataaaaa ttccccaaag ttgaagttac cgtaagccta ttttttatcg tcttttcttt
      301 cttgtaactt tgcttttatc caaataccaa tccctgctaa cataccagcc cctcccccca
      361 cctttaaaga ataaataata tccgccctcc aatcaaaaaa taaaacttga gttttagaaa
      421 aaactatcag tctaccaaga atcgtaccta acattgccat aaaaaagatg caagctatgc
      481 agcgcgttat tagtatgata agaatgtaaa agttgacact cttatttatt tttaattgat
      541 ttatcagctt catctagtgt tccttttgtt acattcccga tagcttcacc agtaattgat
      601 cccccaatat taccaataat atcagatgtt ccctctttga cagtagagcc cattgacccc
      661 gagatgactt tccctatacc gccaccaaat actgaaccta atcctgtccc aataactgaa
      721 tttgtcggat cctcgccttt aatactacta ccaattgccg cgccgctcat gttgataggg
      781 gttgaaacta taattccttt tcctgttgtt gctgctgccg ttacccctgc aattatcgca
      841 tccacataac tgaaaggatc ttttccagca agctgagcag ctgagtttac gctgatacca
      901 attgctgcat tagtcgccat acctttcact gttaacccca gtacgcttcc tccaaacaac
      961 aaaggagcat caccaatact tgcattagat ttatgatacc cccaggtatt cataatgctt
     1021 tgagcctttg ccattgcttc agtatcaggg ttacgtttta tatagtcctg tcctgacagt
     1081 aacccagtaa caagctcctg tgccaccgct gtaccgtact ccttttccat ttctgaagca
     1141 tatacacgca agtaagctgc cagttcagca cgttctgcgc tgtttaattg tgaaggatct
     1201 ttattaaatt tggataccag agcatcacta cgtttatctg cattttccag cttaatcagc
     1261 tcattagcag tggctaatgt cttatcgccg ttcctgattt tctcaatggc tttatccagt
     1321 ttgtcagaag atgtcgctcc cagaaagtta ttctcaacaa ctatttttta tttttaaagt
     1381 aaaactctac ttttgctagc aaccacaaac cactaccaag cagccccccc accccacaac
     1441 ctattttaac aataaaatat
//
LOCUS       DAPHJE010000286         1444 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPHJE010000286 DAPHJE010000000
VERSION     DAPHJE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.289"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..113)
                     /locus_tag="RH222_004570"
     CDS             complement(<1..113)
                     /locus_tag="RH222_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3799463.1"
                     /translation="MNNTDSLEKIIKHQQNNDPKYPCQEHLLIQLCIRVNK"
     gene            complement(366..575)
                     /locus_tag="RH222_004571"
                     /pseudo
     CDS             complement(366..575)
                     /locus_tag="RH222_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            743..1283
                     /locus_tag="RH222_004572"
                     /pseudo
     CDS             743..1283
                     /locus_tag="RH222_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1311..>1444
                     /locus_tag="RH222_004573"
     CDS             1311..>1444
                     /locus_tag="RH222_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3799464.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     "
BASE COUNT          360 a          371 c          325 g          388 t
ORIGIN      
        1 tttttgttta cgcggataca gagttgtatc aacagatgct cctggcaggg gtattttgga
       61 tcgttgtttt gttgatgttt gattattttt tctaatgagt ctgtgttgtt catataatca
      121 tttccatttg ctgaaatact ttgcctttat acaaaagtgc atcagacatg cggtaagcat
      181 ctacttttca caacaagtta tgtctgccag atatctgtat gaatgagaca accaggcaga
      241 aaggaaggat aacttcattc gcataaaaaa acataaatgt ttgttgtggt caacaaaaaa
      301 tgatcacgat tttaaatttt tttccagtat ctattttcca attcgtttgg tagtaacctg
      361 ccattttatt cgtgaggcct gagtgcacta taatacccaa cgatatagtc cgttatgact
      421 tgaactgcat cgctgaagct tacgtacccc accactggta accattcgtt ctttagactc
      481 ctgaagatgc gttccattgg gctgttatcc cagcagtttc cctatcggct gatactttgc
      541 tttatcctgt aacaccagag aagttgttga tatttcagtt cagtatactg acctcttgcc
      601 tggcttgatc aactggtaaa gtacttcacc gttgagcttc ccgtggttga ttttacgtaa
      661 gcgacccgtc agagacgtct tcaggcagtc gagtgaaaac gctaacttag acgcccatct
      721 gaaacagacg gacatctaag ttatggaatc tgaaacccgg gcctggcgga ccgaaccccg
      781 ccgcgtttat tccagcgaat tcaaactcag gctggtcgaa ctggctaccc gaccggacgc
      841 caatgttgcg gatattgccc gtacgcacgg ggtcaacgat aacgtccttt ttaaatggat
      901 ccgtctgtgg cgtaaagaag gccgggtatt ccgccggctg ccagctcccg ttccatctgt
      961 atcccctcct gcggcatttc cggtcgaaat tgtacctgac tccaccaccc ggcagtctcc
     1021 ggctctgtct ggtcagggaa cggggtcaac ctgctcgctg acgcttcgcc acggcaccct
     1081 cacactcgac aatcctgacc cggagataca gctccggatc gtccgcgaac tcgccggcag
     1141 gagcacagta tgattacgct tccggctggc agccgtatct ggctcgtggc cggcatcacc
     1201 gacatgagaa acggcttcaa cggtctggcc gcaaaggtcc agaactgatc ttacccagca
     1261 atagtggaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa
     1321 cagtatcaac cagtaaaaaa ccccgtaaac agcattcgcc tgaatttcgc agtgaagccc
     1381 tgaagcttgc tgaacgcatc ggtgttactg ccgcagcccg tgaactcagc ctgtatgaat
     1441 caca
//
LOCUS       DAPHJE010000287         1433 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPHJE010000287 DAPHJE010000000
VERSION     DAPHJE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.290"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..980)
                     /locus_tag="RH222_004574"
     CDS             complement(<1..980)
                     /locus_tag="RH222_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3799465.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPHR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQF"
BASE COUNT          408 a          326 c          329 g          370 t
ORIGIN      
        1 ataaattgct catagccaac ccatgcggag tcaaacagaa tgtagtcaca cagatgacca
       61 attttatcca ccacctggcg ggcgttataa atcgtaccgt cgtaggtgcc taactgaatc
      121 acagcgaggc ggaacggacg cgcctctttt gcccggtgcg gtgcaacttc tgtaatcagt
      181 tcacgcagat aactttcttc gaagcagtgc gcatcaatac caccgataaa accatacggg
      241 ttacgtgcgg tttccagata aaccggtgtt gcaccagcct gtagcaaggc cccgtggtgg
      301 ttagacttat ggttattgcg gtcaaacagc accagatcac ccggtgtcag cagggcgttt
      361 aaaaccactt tgttggagga ggaggtgccg tttaaaacaa agtacgtttt atcggcatta
      421 aacactttag ccgcatgttg ctgtgcaatg catggcgcac cttcatgaat cagtagatcg
      481 cccattgcta cgtcggcatt acacaagtcg gagcggaaga gcatttcgcc aaaatattcc
      541 acgaactgat tgcctgcggg atggcgacgg aaaaactcac cgccctgatg acccgggcag
      601 tcaaaagcgc tattgccctg attgacataa tcgaccagtg cgcggaagaa gggcgggcgc
      661 agttgcgttt cataatggct ggcagcggtt tctaactgac gtccgtaaaa ctctttccgt
      721 gattcgcaat gctcgaagac accagaaata cgtggcagat attctgctgg tacacgttct
      781 tcattttccg ttgcaataaa aacaggaatg ccgtagccag tagcgtctat ttcatcgagt
      841 tttccacgtt caacatcatt gactgataaa ataatggcgg caacgtcaat ataattactt
      901 tcgtcaatat tcacgcatgc acgatgagtg gaaaaacaat ccgggcaaga acggctaacg
      961 gcaattttta attctgacat tttgttcatc tctttatttc aggtaatagc aggtcctcaa
     1021 tttctcaaag gagaaatcga tcgcgccgga aaaaagcaaa actccacagc tgataaataa
     1081 atcagtagtt tgctttttgc agatgcaata acccagacca gcccggtaac tggcgatact
     1141 catcatactt caagttgcct atgctgcgac tcgaattatg tagagtttaa aagggttagc
     1201 agtatatgag caccgaggga tacgggtatt aacctgtaat gcttaaagtc agggagacct
     1261 gtacgctggg gtgcccttta gtcagaagac taacgggcat taaagaaatg aaagtcaaaa
     1321 ctattacgat gggcaaactt catgatatgt gttacgcgcc tgatatgggg catttttcgg
     1381 ttattgtttt ccatttcata tacctttctg tatcactaga aaacaataat att
//
LOCUS       DAPHJE010000288         1433 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPHJE010000288 DAPHJE010000000
VERSION     DAPHJE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.291"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..193
                     /locus_tag="RH222_004575"
     CDS             <1..193
                     /locus_tag="RH222_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HDV3799466.1"
                     /translation="DNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWR
                     RHSWCGGLSPEQFENKNLA"
     gene            complement(234..>1433)
                     /locus_tag="RH222_004576"
     CDS             complement(234..>1433)
                     /locus_tag="RH222_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3799467.1"
                     /translation="DTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQ
                     IIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISL
                     FFVFGGAIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYF
                     NGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHI
                     FSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTW
                     GHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVE
                     RLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDV
                     VNFCESP"
BASE COUNT          450 a          315 c          263 g          405 t
ORIGIN      
        1 cgataatgcc tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta tccatggaga
       61 acactttatc agccgggaaa taatgcgggc aacggtgttt aattatatcg aatgtgatta
      121 caatcggtgg cggcggcaca gttggtgtgg cggcctcagt ccggaacaat ttgaaaacaa
      181 gaacctcgct taggcctgtg tccatattac gtgggtagga tcaaaatgcc gctttatggt
      241 gactcacaaa aattcaccac atccctcaca accaccttat tacgcccggt ctctttcgcc
      301 tcgtaaagtg cgttatcagc cagtcgatag atctcatttg ggtttaacgc attttcctga
      361 gtcacaacag cgccaatact aatcgtcact ttttgtggta tagcgtattc aggattatcg
      421 ccagttaaac gctcaacatt ttcccgaatc ctttccgcta aagctttcgc acgttcagta
      481 tcgatgtccg tcagcaagac gccaaactct tcgccgccga cgcgcgctaa aaggtcatct
      541 ggtcgtatgc ttttcccaat gatattcacc actgttttta tcacctgatc gccaaccgga
      601 tgcccccagg tgtcgttgac ttttttgaag tggtcgatat ccataatcat gacgcaataa
      661 ggcgtttttt gggctgatgc tgattgaact gtcagttcat taaaaaaata atttctgtta
      721 aatatattgg ttaagggatc gcgatgtgca atgttctttg ttactcgtag cgcactgaat
      781 atatggcaca taaatattac cataacggtt aacttactga ctacttcaat agtgcgacta
      841 atataccaag tggacacgct atacctgctc ataaataaca gagagatatt gtacagtacg
      901 gcacaaaaag caatcaccgt cactccattc cagatatcat agcgtaaacc attaaagtat
      961 agattaaccg aaagcaaggt taaccacatg aaaatcatta tggtaacata tgaggctttc
     1021 caaaccacct gaccattttc attggttaac tcggcaatgt gtaaattgta gctctcataa
     1081 tgactagaaa gtatgtgagc aactatcgcc cccccaaaaa caaaaaacaa acttatgcac
     1141 aaagtcattt ttttggaaaa taaattgcgc tgttttgtgt tttcactaac tttattcacc
     1201 aatgccagaa atattaatat gcacaaactc atctgacgaa acaaataata gattgcaatg
     1261 tcattttgga caacactgct tgtcaaacgc tcctcaataa tttgctgaat gataatgacg
     1321 gttttaacaa agtaaatcaa acaactcaga aaagccaaac taagtacaca gtttgaaaat
     1381 tgcctacggt cataaatgaa atacaactgc ataaaaataa atgcaacagt atc
//
LOCUS       DAPHJE010000289         1385 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPHJE010000289 DAPHJE010000000
VERSION     DAPHJE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.292"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1340)
                     /locus_tag="RH222_004577"
     CDS             complement(<1..1340)
                     /locus_tag="RH222_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDV3799468.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGDLYSHRWEIELGYREIKQTMQRSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSV"
BASE COUNT          282 a          392 c          355 g          356 t
ORIGIN      
        1 actgactggc tctttttcgg ggctgtgggg tatttccagg gcctctcctt taccactctc
       61 gggaaggccc tttcccttct tgtcggtaat ttcacaagtt gtcccatact tgcaagatcg
      121 cgcatcagct ccggtatacg tcccggtgaa gcgccctgca atgtcatcag cattctcatc
      181 accattccgc atgattctga gaaactcagt tgattcggcc agtaaccttt cagatgttcc
      241 gccattttaa tcatctgata tctcaccaga ttataagcca gtaagacacc ccacagctct
      301 tgctccacaa gctccggctt tttacttctc agcgtcagcc tgctccgttg catcgtctgt
      361 tttatctccc tgtatcccag ttcgatttcc cagcgatgac tgtacagatc ccccatttct
      421 cctccgggga agcgcatggc gtccgtcatc gacgtcagca gatggcagac ttttcctttg
      481 cgcgtcacgg tcagcaggcg ggcagtcact tcatttccca gtcccggcca cttttttcgt
      541 gcctgcgggc tggttttcag cttcaccaga tgatcgcctt tacccagttt tctgatctct
      601 tcatattgcg ctccctttct gagaggtatc atccagtggc ggtgttctcc cgccaggctc
      661 caggcattta acagtcccag tgagtaataa cctttatcca ttaacgtcag agtgttatcg
      721 ccggtttgtt ctataagttg ctcagcaagc tcattttcgc tgttcttcat cgtgccgaag
      781 gctgcagccg tcagcagatg gctggtcagt tccatctggc agaccatttt gacctgcggg
      841 tagagcgccg ggttcccggc atgtgtctgg cgggggaagg ctgcatcgtt ctctggtgta
      901 tccggtgtgc gccagaacac accatcgatg gccagcaggg tcaggccgca ccagtgcgga
      961 tgcggcgtgg cgttatgcca gagctgcgct gttttcgtga acacgcggcg gacagcctca
     1021 cttcccaggc gctggcgggc ctgaataacg gcactggggg caacgaaggg gcgattgccc
     1081 ggcagcatga tgtccaggcg attcacaatc tggtgaagag gttctttacg ctcaagcgcc
     1141 atgccaacaa tacaccagac catcatttcg aggggaagac ggcgcttgcg tagcgttaca
     1201 gtacctgatt cggcaaggca acgagagatg agttcggggt cgaggtaatc ccccagagaa
     1261 gtcagtgggt tacgcagaga atcgtaacgg gataccagat caagagcctg tccaatgtgc
     1321 ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc
     1381 aactg
//
LOCUS       DAPHJE010000290         1379 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPHJE010000290 DAPHJE010000000
VERSION     DAPHJE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.293"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..838)
                     /locus_tag="RH222_004578"
     CDS             complement(<1..838)
                     /locus_tag="RH222_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3799469.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIG"
BASE COUNT          463 a          295 c          210 g          411 t
ORIGIN      
        1 caccaatctt atcaccaaca aaaccaaaaa atattgtacc tgtcagtgtt cctaaacctg
       61 ctattgtcat cagagttgaa atgaccactg tattaactcc attatctgcc aggtaggaag
      121 gaagtagtcc gtttatcggc cagtttgcac caaatagaca aaaacagacg aggaaaacga
      181 tcatagagat tgaaagatgt ggttttctga agacagacaa aaatgttgat ttatccttat
      241 atttatcttc aatccactcc tgactttctg gagcactttt tctgatccaa agaactagta
      301 aaactggtaa caggcctata aaaaaagagt ttctccatcc atatacttca gcaaactgag
      361 ggattatttg tgccgcaata atatttccaa cagaaaaacc acttaccaaa aaagcactag
      421 ctttagattg aagattttta ggccaacttt ctaccgcata agttgaagca catgcatatt
      481 caccagacat ccctaagcca acaataaaac ggcaaactgc gagcatatat aagtttgtag
      541 caataccgct aaggcctgtt ccgactgagt aaatgaaaat tgcccacatc atcattggct
      601 tacgaccata tttatcagcc atggcaccaa aaaaaccacc tccaataggt ctggctatga
      661 aggccactgt ccctattaaa gtagcctgaa tatccgtaat gccaagatct gcttttataa
      721 tatgaagaat gtaaaatatc atcataaaat caaagccatc aaatacatat ccaagccatg
      781 cggaaaaaag agctttccgt tgtggtggat taacttgttt ataccatgct gttgccatat
      841 ttgtttcctg tgttattcta atatagccct taaagtaaat attagggagc ctcaacaata
      901 tccagaaata attatattgt cttcaaatac gccagtacta ttgattgtta tcaataccta
      961 atcagtacaa tcttttcctc catttcttct taacatattt cactctatga aagataaaac
     1021 tgatacccac ctacagtttt gggtcaccaa caaaaaacaa tgttcgccag atattccacc
     1081 actggcacag ttcaaccctg cttcaaaaat tgacttattc attgtgctca ttttcgtagt
     1141 ccgttcaggt tgaataaaac tcccttcgtc aaatgaggaa agactgagag gttattactt
     1201 tagcttacgt tatagctgtt ttcctttgca gtatttcttt gcttttctgt atacctttat
     1261 acctgttata ccagatcaaa aaacaagcaa tccacataac aaaacgcgtt ttgttactga
     1321 tgtcacaaat tgagcatatt tttgtagctg atagtttgtt acaaacacca gaaccaaga
//
LOCUS       DAPHJE010000291         1373 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPHJE010000291 DAPHJE010000000
VERSION     DAPHJE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.294"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..1161
                     /gene="gmd"
                     /locus_tag="RH222_004579"
     CDS             43..1161
                     /gene="gmd"
                     /locus_tag="RH222_004579"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV3799470.1"
                     /translation="MMKKALITGVTGQDGSYLAELLLSKGYEVHGIKRRASSFNTERI
                     DHIYQDPHESNPKFHLHYGDLTDTSNLTRILSEVRPDEVYNLGAMSHVAVSFESPEYT
                     ADCDAIGTLRLLEAIRFLGLEKTTKFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRGLSYISQGLEKCLYM
                     GNLDSLRDWGHAKDYVKMQWLMLQQETAEDFVIATGIQYSVRDFISMAALELGITLKF
                     EGEGVDEKGIVASVNNEISPAVNVGDVIVAVDPRYFRPAEVETLLGDPTKAKDKLGWV
                     PETTLQEMVTEMVASDLEAAKKHSLLKKHGFNVNISME"
     gene            1165..>1373
                     /locus_tag="RH222_004580"
     CDS             1165..>1373
                     /locus_tag="RH222_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV3799471.1"
                     /translation="MSRKRIYIAGHNGMVGSALVRNLETRNDVELILKTRQELNLLDQ
                     QAVNNFFKTEDIDEVYLSAAKVGGI"
BASE COUNT          431 a          239 c          279 g          424 t
ORIGIN      
        1 caatgtttga aaacatttta aattaaccaa taattggaat agatgatgaa aaaggcgtta
       61 attactggtg taactggtca agatggatct tatttggctg aattgctttt gagtaaaggg
      121 tatgaagttc atggtatcaa acgcagggcg tcctcattca acaccgaacg tatcgatcat
      181 atctaccagg atcctcatga aagtaatcca aaattccatc ttcactatgg tgatttaact
      241 gacacatcaa acttaacccg tattttatct gaagtacgtc ctgatgaagt ttataattta
      301 ggggcaatga gtcatgtggc tgtttcgttt gaatcacctg aatataccgc cgattgtgat
      361 gcaataggaa ctcttcgtct tttagaggct attcgctttt taggattaga aaagacaact
      421 aaattctatc aagcctctac ttcagaactt tatgggttag tacaagaaat acctcagaaa
      481 gaaacaacac cattttatcc acgatctcca tatgctgtgg caaaactcta tgcatattgg
      541 attactgtaa attaccgtga atcttatggc atgtatgctt gtaatgggat tttattcaac
      601 catgaatcac cacgtcgtgg tgaaactttt gtgaccagaa aaattacgcg tggtttatcc
      661 tatatttctc aaggtttgga aaaatgccta tatatgggta acttagattc attacgtgat
      721 tggggacacg ctaaagatta cgtgaaaatg caatggctta tgcttcaaca agaaacggca
      781 gaagattttg tcattgcaac tggtatccag tattcagttc gcgattttat atcaatggcc
      841 gctctagaat tgggtattac tcttaagttt gaaggagagg gtgttgacga gaagggaatt
      901 gttgcttctg taaataatga aatctctccg gctgtcaatg taggtgatgt cattgttgct
      961 gttgatcctc gatattttcg tccagctgaa gtcgaaacat tactgggcga tccaactaaa
     1021 gctaaagaca aacttggttg ggtaccagaa acaacattac aagaaatggt aaccgaaatg
     1081 gttgcttctg accttgaggc agccaaaaaa cattcactcc ttaagaagca tggcttcaat
     1141 gtaaatatct caatggagta aattatgtcc cgaaagcgta tatatatagc aggccataat
     1201 ggcatggtag gttcggctct tgttcgtaat cttgaaacga gaaatgatgt tgaacttatt
     1261 ttaaaaacta gacaggaact taatttactc gatcagcaag cagtaaacaa tttcttcaaa
     1321 actgaagaca tcgatgaggt ttatctctct gctgctaaag ttggtggtat tat
//
LOCUS       DAPHJE010000292         1369 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPHJE010000292 DAPHJE010000000
VERSION     DAPHJE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.295"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..928
                     /locus_tag="RH222_004581"
     CDS             <1..928
                     /locus_tag="RH222_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV3799472.1"
                     /translation="HDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLR
                     HSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTD
                     AMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPAHDSSAMSEKSH
                     GREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSA
                     DLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTN
                     DKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            958..>1369
                     /locus_tag="RH222_004582"
     CDS             958..>1369
                     /locus_tag="RH222_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799473.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAKLLNA
                     QSQMEFWREPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQ"
BASE COUNT          428 a          234 c          373 g          334 t
ORIGIN      
        1 tcacgatacc attgccagag ttgtatcctg tatcagtcct gcaaaatttc acgagtgctt
       61 tattaactgg atgcgtgact gccattcttc agatgataaa gacgtcattg caattgatgg
      121 aaaaacgctc cggcactctt atgacaagag tcgccgcagg ggagcgattc atgtcattag
      181 tgcgttctca acaatgcaca gtctggtcat cggacagatc aagacggatg agaaatctaa
      241 tgagattaca gctatcccag aacttcttaa catgttggat attaaaggga aaatcatcac
      301 aactgatgcg atgggttgcc agaaagatat tgcagagaag atacaaaaac agggaggtga
      361 ttatttattc gcggtaaaag gaaaccaggg gcggctaaat aaagcctttg aggaaaaatt
      421 tccgctgaaa gaattaaata atccagcgca tgacagttcc gcaatgagtg aaaagagtca
      481 cggcagagaa gaaatccgtc ttcatattgt ttgcgatgtc cctgatgaac ttattgattt
      541 cacgtttgaa tggaaagggc tgaaaaaatt atgcgtggca gtctcctttc gctcaataat
      601 agcagagcaa aagaaagagc ccgaaatgat ggtcagatat tatatcagtt ctgctgattt
      661 aaccgctgag aagtttgcga cagcgattcg aaatcactgg cacgtggaga ataatctgca
      721 ctggcgtctg gacgtggtaa tgaatgaaga cgactgcaaa ataagaagag gaaatgcagc
      781 agaattattt tcagggatac ggcacatcgc tattaatgtt ttgacgaatg ataaggtatt
      841 caaggcaggg ttaagacgta agatgcgaaa agcagccatg gacagaaact atctggcgtc
      901 agtccttgcg gggagcgggc tttcgtaatc ttgccctggg taggaggccc tccagggatg
      961 ttagtcggtg cggcagttgg agcggtggtt attggtggtg gtatagtaat atacaacaat
     1021 cgcgaggctg ttggggatgc gattgagaaa agtatggaga tgcggggtga agccaaatta
     1081 ttaaatgcac aaagtcaaat ggaattctgg cgagaaccct atggtgcttt ttccagcgtg
     1141 tttggaaatg atgattcatc aaaagagaat ccaaatgtcg ggaaggattt gacggatgaa
     1201 gagaagaact gtttgggcag tggcagtaca ggaggttcgg gagggtggga gccagatgag
     1261 gatggcgaaa taagaaatac ctacaggtct attaaggatg ctcctcagta tcccagaggg
     1321 gttaggaatg tccaaaatgg cactacaagg aatgttgtaa aggatcagt
//
LOCUS       DAPHJE010000293         1305 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPHJE010000293 DAPHJE010000000
VERSION     DAPHJE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.296"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(175..1302)
                     /locus_tag="RH222_004583"
     CDS             complement(175..1302)
                     /locus_tag="RH222_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDV3799474.1"
                     /translation="MSCEALNIEFMQMLKGICVEDDIEFNFFELGGSGYLENPTTDLM
                     ALFMGAQKMVPPWLLKALLCCLDGSLDVDEIDFTSLELMREVRTEDGKYLDILIRHDE
                     FIIGIEHKVLADTYNPFPSYVSLIDSYGGNNQNLFRCILKPDGNCAKGVDGWQLIDYS
                     LLLETAIRRLGLEMMNQEFSKWAVFYQEFLSHLKKLSEVSMDKVSDKNVEFVTENFSA
                     LIKSVQLLEMYQNAITEEAKSVVSEVLPDIHIATGINNWKGYYKAIHLMPGCWGQGKT
                     GVTLVYRPSEDGRDEAEFYVYGWIHSDDYPEINALKEEIRVALSAGDFIPTASSEDYE
                     VSITGKGKVLELSFWGNTRSKKDALVLLKDMTEWMDSKIRT"
BASE COUNT          402 a          313 c          184 g          406 t
ORIGIN      
        1 aaactccact acaccaggga caattaacct caaaagtcgt tctactgcaa gtatcacact
       61 gataaatcat ttgaatattc tccttttatt ccaatatctg actattcgac actttaattt
      121 cgtgcctaat aatctacaat ttaaaaaaga tcatcataac gcagtcatat taatttatgt
      181 tcttattttt gagtccatcc actccgtcat atccttaaga agaaccagtg catctttttt
      241 gcttctggtg ttcccccaaa aactcagttc aagcacttta cccttcccag taattgatac
      301 ttcgtagtcc tcagagctag cggttggaat gaaatcacca gcacttaaag caactcgtat
      361 ttcttctttt agtgcattaa tttcagggta atcatctgaa tgtatccatc catatacgta
      421 gaactcagct tcatctcgtc catcctctga agggcgataa actaacgtaa ctcctgtttt
      481 accttgcccc cagcatcccg gcatcagatg aattgcttta tagtaaccct tccagttatt
      541 aatacctgtt gcaatatgaa tatcaggcaa tacctctgag acaaccgatt tagcttcctc
      601 ggtgatagca ttttggtaca tttccagcag ttgaaccgat tttatcaaag ctgagaaatt
      661 ttcagtgaca aactccacat ttttatcaga taccttatcc atactaacct cactcaattt
      721 tttaagatga cttaaaaact cctgataaaa aacagcccac ttgctgaatt cttgattcat
      781 catctccaac cccagacgtc gaatggcggt ttccagcagc agtgaataat cgataagttg
      841 ccaaccgtct acgcctttag cgcagttacc atcaggcttt aatatacaac gaaatagatt
      901 ttgattgtta ccaccgtaac tatcaataag acttacatag ctgggaaaag gattatatgt
      961 atcagccaac actttatgct caatacctat gatgaattca tcatgtctga taagaatatc
     1021 tagatatttc ccatcctctg ttcggacctc tctcattaat tccagagagg taaaatcaat
     1081 ttcatcaaca tcaagggaac catccaggca acatagtaat gcttttagca accatggcgg
     1141 caccattttc tgtgctccca taaacaaagc catcaaatcc gttgttggat tttcgagata
     1201 tccggaaccg cccaattcaa aaaaattgaa ttcaatatca tcctcaacac aaatcccttt
     1261 taacatttgc atgaattcga tgtttaacgc ttcacatgac acttt
//
LOCUS       DAPHJE010000294         1287 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPHJE010000294 DAPHJE010000000
VERSION     DAPHJE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.297"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            169..672
                     /locus_tag="RH222_004584"
     CDS             169..672
                     /locus_tag="RH222_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV3799475.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(751..1002)
                     /locus_tag="RH222_004585"
     CDS             complement(751..1002)
                     /locus_tag="RH222_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV3799476.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1117..1203)
                     /gene="azuC"
                     /locus_tag="RH222_004586"
     CDS             complement(1117..1203)
                     /gene="azuC"
                     /locus_tag="RH222_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV3799477.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          344 a          296 c          286 g          361 t
ORIGIN      
        1 aagcattttc ttatacccgt tcagacgtta ttcttatttc agatcatcgt cagaattgac
       61 tccacgatca catttcggac cggcagaaag gaattattct gcaaacagta attatggtgt
      121 tttgatttat cttgcacctc tccacttctg gatataagga tattaggtat ggcaaccgct
      181 ggaatgcttc tcaaactcaa ctctcaaatg aaccgcgagt tttacgcatc caatctctac
      241 cttcacctga gtaactggtg ttctgaacag agtctgaacg gcaccgccac tttccttcgc
      301 gcccaggcac agagtaatgt gacccaaatg atgcgcatgt ttaactttat gaagagtgtc
      361 ggcgctaccc ccatcgttaa agccattgat gttcccggtg aaaaactgaa ctctctggaa
      421 gaactgttcc aaaaaacgat ggaagaatac gagcaacgtt ctagtacgtt ggcacagtta
      481 gccgatgaag cgaaagaact gaatgatgat tcaaccgtca atttcctgcg cgatctggaa
      541 aaagaacagc agcatgatgg tctgttgctg caaaccattc ttgatgaagt gcgcagtgcg
      601 aaacttgcgg gtatgtgccc tgtgcagacc gaccaacatg ttctgaatgt cgtgtcacac
      661 cagctgcatt gatcatcatc ggcgctaatg cattgcgccg atgaaggttt tgagaaaccg
      721 ctgcctcatc tgtttgaagc agcggttttt ttaatgggat tcaccctgtg gggtaaactt
      781 gagttcaata agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt ctgcggcggc
      841 gggatggtta gtaaagtcga tattcttcag ctgactggac attttttcac gaacttcaac
      901 gggcgcgatt acatcgagaa catccagaat ttgtttgata accaactggc aagcaaccac
      961 atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact cctgatagca
     1021 ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat ccaccgtcat
     1081 caggacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat tgcctggcgg
     1141 tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggattt tgcgcagttt
     1201 catcgcggca ctccgactat ttgttataca ggtgttattg tctttgcgct tataatatga
     1261 caaccatcac aaaaatcaat ctttatg
//
LOCUS       DAPHJE010000295         1286 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPHJE010000295 DAPHJE010000000
VERSION     DAPHJE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.298"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(663..902)
                     /locus_tag="RH222_004587"
     CDS             complement(663..902)
                     /locus_tag="RH222_004587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799478.1"
                     /translation="MEREFPEALERMKERLPLYLIMVLENQYNRLNELDSLIEDIEKQ
                     LTSVAKQNETCKRLLDIPGVGPLIATAAVAIIGEA"
BASE COUNT          331 a          326 c          285 g          344 t
ORIGIN      
        1 cataaaaata cggaaaaccg gattgaagac agatatgcct gcagacctgg agggccgtgg
       61 cgggatatgg ggctttatat gaatgcatcc cgtttacaca gcttccattg ttctggcatt
      121 gacggtaggt tgcagccttt atatccggca ttattgcagc cataaagctg cgagccccta
      181 tgtaattcgc tgactcacac ttcatttact gcgcgggatt caagccaaaa aaacattcag
      241 gtttagtgag tcccggtctt acctgtcttt gtcatcacta cggctgccct cgcaaccttt
      301 cagcatcccc aattttgtta agagttcatt gttgaacggt atcagcgatt aatatggtct
      361 gatactgaca tggttcccgt catacttacg accatgtgcg gctattgccc acactgttcg
      421 tgccagcttg tttgccatgg cgacgttgcc acgccgaccg gacgacgttt tttcagttcg
      481 gttatccacg gcccaggttc tctggtcagt aatgctgccg cccttgcacc atgaataaac
      541 agggttcgca gatatttatc accacgtttg cttatccccg gcaggcggat tttccctcat
      601 gagccagtct gtcttggcac cagaccagca taagccgcga actctcgccc tgatttaaac
      661 gcttatgctt cccctatgat agccaccgct gccgtcgcaa taagtggccc aacgccggga
      721 atatccagca accgcttaca ggtctcattc tgtttcgcca cgctggtaag ctgcttttca
      781 atatcctcta tcagtgagtc cagctcattc agtcggttgt attggttttc cagaaccata
      841 atgagataca gtggcagtct ctctttcatc cgctccagcg cttcagggaa ctcccgttcc
      901 atcgctgccc ggcctttgtg gatggtttta ccaaactcca gtagcgtccc gtgcagggca
      961 ttaacggtct aacatcgtgg cgttgacacc aataaaagga gaaataaaaa tacctaccat
     1021 aataaatggc agataaaact gtgataaggg aggaaaacag gtaggtgtat gctctcctgt
     1081 cggtcatcag gtaatctgat acaagaagat actgttcagt taaaccgcgg gagataatct
     1141 gttatgaaat atgtgcgtca cttctcagaa atcgtgtaat aacaaacaaa attaatcaaa
     1201 agaaattcat cgcaatgata tcccaatatg cctgtgagca tataaaaaaa gcccgaggac
     1261 acttacgggc tgattgagcg acattg
//
LOCUS       DAPHJE010000296         1271 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPHJE010000296 DAPHJE010000000
VERSION     DAPHJE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.299"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(606..704)
                     /locus_tag="RH222_004588"
                     /pseudo
     CDS             complement(606..704)
                     /locus_tag="RH222_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            complement(794..1162)
                     /locus_tag="RH222_004589"
     CDS             complement(794..1162)
                     /locus_tag="RH222_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV3799479.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
BASE COUNT          345 a          283 c          325 g          318 t
ORIGIN      
        1 catgatcctt acaggaatca gcagaggaaa acatctatta ttaccatgta attcatgttg
       61 ttatgtcgca acaatagtac tgcacgggga attattacgg gcaaacttag gcgaaaggaa
      121 gtactgaagt gacagaataa atcacacttt ttgtacatat gtacttttct attgtctttt
      181 gttgtggaaa caaatggtgt ttgtcatgcc gatgctaata gacagataga ttcgagggcc
      241 aacagcccca tcatcatgat ggagcaacag aggtgagtct aacgtttccg cacttcttat
      301 cgatcggcac taacgatcaa tatcgataaa ctgatctatc aaaacgatta tctttcttgc
      361 aacctgccta gttatccttc tcttacattg ctcgcagaaa agccctttta ttacggttag
      421 ttatctagct gaggcttgct acgcccattt tccatgtacc ccaaatcacg aggtcacaaa
      481 ggcgcggaaa gatgaaggga tgtgcacgtt gcaacaaaaa aaagaagcgt ccttgaacag
      541 aggctctcac tcgataaaga attctgcgac gagtgcttat tcgatatcga tgaggaaatc
      601 agggaatgct ggtccagtaa ccgggtgagc agtgtctgcc agatggtgac tggagacggg
      661 cagcacgata cctcccgtac atgcgtgtcg ggtaatgttt tcatactgaa gattttcctg
      721 atatgcagag ataaaaaatg ggacagtggc gtggtgaaaa caccaggccg gagcagaagg
      781 ttattctggt ggtttacttt ttcgtttcag gtgtcggata aacagccaga taaacgtaac
      841 cacaggagcc gagggtgtcg gcttcacagg tcagcccctt cgcatacagt gtcacggtat
      901 gctgatggcg gggattcagt tcaccactgg tgagcattaa tttcagttgt ttcatcagca
      961 gcggaaaggc ctggtccaga gggtacgcat ccgcatctct gaaccggcct ctgataccgg
     1021 cacggtcggc aaggtaatgc aaccggttac cctcctgcac cagacgggcg ccgaaacagg
     1081 gcgtcactgt gcagggtagt ccccaccagg gatggtcgtc attgtcatcg ggatgcgttg
     1141 tcccggaaag tgtgtctgac acgataaaat cctcacagaa aatcggtgaa tgatagttta
     1201 acgatgacgg gtcagccggc gcgcagaccg gcttcggtaa aggtgtattc gttgatgagc
     1261 aggacctcat c
//
LOCUS       DAPHJE010000297         1235 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPHJE010000297 DAPHJE010000000
VERSION     DAPHJE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.300"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..498)
                     /gene="sfmF"
                     /locus_tag="RH222_004590"
     CDS             complement(<1..498)
                     /gene="sfmF"
                     /locus_tag="RH222_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV3799480.1"
                     /translation="MRRALFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIF"
     gene            complement(509..>1235)
                     /gene="fimH"
                     /locus_tag="RH222_004591"
     CDS             complement(509..>1235)
                     /gene="fimH"
                     /locus_tag="RH222_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV3799481.1"
                     /translation="YKYMQLNDYLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLP
                     FGVMDSRLIFRLKVIRPFINMVEISRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVP
                     QNCELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMR
                     LEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPI
                     SITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          345 a          326 c          272 g          292 t
ORIGIN      
        1 aaatatcgca tccgctttcg ccacacctgg ccgaacaccg ctggctaacg ctcgataatt
       61 ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg ggctcgcctc
      121 ccccagtgcg agccgggagc gatcgctatt acgtaattcg atggcgacgg tttgtgccat
      181 tgcgggatca tccagagcca gcaggttggt atcggatgcc ggcgttcccg taaataaaat
      241 cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggacga gtgccgtggt
      301 atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg tcttatcaat
      361 atccgcagtg tttacggtac aggagaaatc aacaacgtta ccgtgcaaat tgatattaat
      421 cgttcccaaa gggtcagctg cccatccact ggaactccac agtaacccgc agaaacagct
      481 aaagagtgct cttctcattc tccgtacctc attgataatc gacgcgtaaa taccccagcg
      541 cgctaaacgg cccttcggtc ggtttttgac cggtaatact gataggccag gcgcgaagtg
      601 tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg agatcgttag
      661 gcgtgatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga ttgtccgaca
      721 ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa gcctgcgcag
      781 caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacacca gcaggtcgat
      841 tacccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca aattcaacaa
      901 tctgcccggc atttaattcg cagttttgcg gtacttcaac ccggccacca aaactaatgg
      961 tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg gtaaacatca
     1021 cctgtctgga gatctccacc atgttaataa agggacgaat aacctttaaa cgaaatatta
     1081 aacgagaatc catcaccccg aatggaaggt tttgcgaaac gttaggatct gttcccatca
     1141 gaatataatt ttgcgggggg aactgaatat ccatcacact atcaaccaga ctcatcgcac
     1201 ctaatagata atcatttaat tgcatatatt tataa
//
LOCUS       DAPHJE010000298         1227 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPHJE010000298 DAPHJE010000000
VERSION     DAPHJE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.302"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..1088
                     /gene="ompC"
                     /locus_tag="RH222_004592"
     CDS             6..1088
                     /gene="ompC"
                     /locus_tag="RH222_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063070036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3799482.1"
                     /translation="MAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSN
                     DADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKF
                     GDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGL
                     VDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRTDTQVN
                     AGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKAQNFEA
                     VAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVDYKINL
                     LDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          340 a          273 c          267 g          347 t
ORIGIN      
        1 taacagtggc aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag
       61 ctgaaattta taataaagac agtaacaagc tggatctgta cgggaaagtt aatgccaagc
      121 actacttctc ctctaatgat gcagatgatg gtgatactac ttatgcccgt cttggcttca
      181 aaggtgaaac ccaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca
      241 aaggcaaccg cgctgaatct caaggttcct ccaaagacaa aacccgtctt gcatttgcag
      301 gcctgaaatt cggtgactac ggctcaatcg attacggccg taactacggt gtagcatacg
      361 acatcggtgc gtggactgac gttctgccag aattcggtgg cgatacctgg acccaaacag
      421 atgtgttcat gactggtcgc accactggtg ttgcaaccta tcgtaacaac gacttctttg
      481 gtctggttga tggtctgaac tttgctgctc agtaccaagg caaaaacgat cgtagcgatt
      541 tcgataacta cactgaaggt aacggtgatg gcttcggttt ctctgctacc tatgaatacg
      601 aaggattcgg tatcggtgca acttatgcga aatctgatcg taccgacact caagttaatg
      661 cagggaaagt tcttcctgaa gtatttgctt ccggtaaaaa tgcagaagtt tgggccgcag
      721 gtctgaaata tgacgctaac aacatttacc tggccactac ctattctgaa acccagaata
      781 tgactgtatt tgctgatcac ttcgttgcta ataaagccca aaacttcgaa gctgttgcac
      841 aatatcagtt cgatttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggtaaag
      901 atcttggagt atggggcgat caggacttag tcaaatatgt tgatgtaggt gcaacctatt
      961 acttcaacaa aaatatgtct actttcgttg attacaaaat caacctgctt gacaaaaatg
     1021 acttcactaa agcactcggt gtaagcactg atgacatcgt tgctgtaggt ctggtttacc
     1081 agttctaatc tgattacgaa aaagatatgt tgcgggaggc gttgcctccc caacatataa
     1141 gtggctccct caagccactt cctttagaag cacaaccttg cttctaacta tataaacctt
     1201 ctgttatata ttacccttta tttttgg
//
LOCUS       DAPHJE010000299         1208 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPHJE010000299 DAPHJE010000000
VERSION     DAPHJE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.303"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            481..630
                     /locus_tag="RH222_004593"
     CDS             481..630
                     /locus_tag="RH222_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDV3799483.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          350 a          240 c          329 g          289 t
ORIGIN      
        1 acggggcggt tacgatgggg cagaccgtgt ccggtgggca ggcgacaggt atcaccgatg
       61 ggataagact gtctgattgg tattataaat atataagaga gatcaaacac atgagatcaa
      121 atacatgagt tcatatatgt gaaataaaca cggtgagtgt gatcagtggg catgatgaga
      181 acgcagtagc aacagactgc gctatccctg atagctggcg tataacctgc gtatataaaa
      241 cgtaccagca acgcacgtga acagagtgca ttaagcatcc gtcgttgaca ccgacctggt
      301 gagtgacagt ctgagagacc ggttaagtgg tggtacggga gtaatgagtt tggggagggt
      361 accagaatgt cagtctggta cggtatgctg acagtatcac gttaattgca gcataaagtg
      421 atgagaggct aatggaagtt aaaaccagag cagttccgtc gttgttcatc ccgggagacg
      481 ctggaaaaag tgatttccca cacgcgttat aagcttactc cagcggagat ggaagccttc
      541 aactctgcgg tcgatcaccg actggcagaa ctgacaatga acaagcttta cgatcgtgtt
      601 cccgcttcag tctggaaata tgtcatctga gcgttcggta atcacaactg catcgcgcca
      661 ggtgttatcc gctttgtgat tcttattggc tcttctcagg ttattaaaag ctgaatatgt
      721 tcctgttgtc gggaaccagg ataataccgg gaacgttaag cggtatagcg acaacaaaga
      781 attcgaaatc aaaagggaat ggtgccggag gcgtgggcgg taatgggctg attttcaggt
      841 attaccggct tacagcgtgt ggttgatatt gaggggctgt gagtttcacg tatttgtcag
      901 aaacccagac gagttttcct gtatgggacg aaataaggca gcggcaatac atggaaaaat
      961 ttaatatggt atgaaggaga acctgtcggt tacgcttaat ataaccggta cactgtcagc
     1021 tcaggggata catgaaccgt tatctcacca gctacacagt atatacacac tgacgctgac
     1081 agtagtcagc aggaccacaa taatcagtgc cagcagtatg gtaacgatac ggtctgtatt
     1141 ccggtaatag ccgaaggggt cgacagaacc acaatacggg caacacacag tattatgagt
     1201 aatcgcgt
//
LOCUS       DAPHJE010000300         1186 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPHJE010000300 DAPHJE010000000
VERSION     DAPHJE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.304"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            34..>1186
                     /locus_tag="RH222_004594"
     CDS             join(34..325,325..>1186)
                     /locus_tag="RH222_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3799484.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTP"
     misc_feature    315..431
                     /locus_tag="RH222_004594"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          288 a          283 c          363 g          252 t
ORIGIN      
        1 gagactaaac ttcctgagaa agaggtaaac aggatgacta aaaatactcg tttttccccc
       61 gaagtccgtc aacgggcagt ccgtatggtt ctggaaagtc aggatgaata tgactcacag
      121 tgggcggcaa tttgttccat tgccccaaag attggctgta cgccggagac tctgcgtgtc
      181 tgggttcgcc agcatgagcg ggataccggg ggcggtgatg gtgggctcac cagcgctgaa
      241 cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc
      301 cttcgccagg cttccgctta ttttgcgaag gcggagttcg accgcctctg gaaaaaatga
      361 tgccactgct ggataagctg cgtgagcagt acggggtcgg accggtatgc agcgaactgc
      421 atattgcccc gtcaacgtat taccattgtc agcaacagcg acatcatccg gataaacgca
      481 gtgcccgtgc gcagcacgat gactggctga agagagagat acagcgcgta tacgatgaaa
      541 atcatcaggt gtacggtgtg cgtaaagtct ggcgtcagtt gttacgggaa ggaatcaggg
      601 tggccagatg tacagtggcg cgcctcatgg cggttatggg acttgccggt gttctccggg
      661 gtaaaaaggt ccgtacgacc atcagccgga aagccgttgc cgcaggcgac cgcgtaaacc
      721 gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc tgattttact tacgtcagca
      781 catggcgggg cttcgtctat gtggcgttca tcattgatgt gtttgccgga tacatcgtgg
      841 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
      901 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      961 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
     1021 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
     1081 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccacactc acgtgggtgg
     1141 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcc
//
LOCUS       DAPHJE010000301         1167 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPHJE010000301 DAPHJE010000000
VERSION     DAPHJE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.305"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..>1167
                     /locus_tag="RH222_004595"
     CDS             67..>1167
                     /locus_tag="RH222_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3799485.1"
                     /translation="MTWLLQEVENGSPIRVVPFAINKNSKIPSVSRISDIIKEQGLFE
                     GLEVTDFYILSEPEQTVSSSKLSENYEREIVSEIILADDFMDYKPCQPKDFVGREYIQ
                     KEVWDYLENVRGNSSQSRVLSLIGGSGNGKSSLIARLSSRFKNQKWKNKFFLTPVDVR
                     SARGGRFVAEAVVKAFSSAIKEGFIEYEKPFLIESVTDIIGSESVQECLKYLSEHDKV
                     LTIFFDQFEEVFMKEELFGLFKEFERFALDVSALQSNLVVGFSWRTGITLGDENPAYS
                     MWNRLKDYRVEKKLEPFDLKDSSKLINSFELNTGFKLNKPLRTRLIQQAQGYPWLLKK
                     LCIHVFKKLKGGISQDQMLVSQLQISNLFHEDL"
BASE COUNT          394 a          174 c          242 g          357 t
ORIGIN      
        1 attccaacag atgacattcc ggcaaaatat actagtgcaa cacttttagt tcatcctgaa
       61 atagaaatga cttggctttt gcaagaagtt gaaaatggta gccctataag agtggtgccg
      121 ttcgctataa ataaaaatag taaaattcca agtgtcagtc gcataagtga tattataaag
      181 gagcaagggc tttttgaagg gttggaagta actgattttt atatattatc tgaaccggaa
      241 caaactgttt cgtcttcaaa attatctgaa aattacgaaa gggaaattgt cagtgaaata
      301 attcttgctg acgattttat ggattataag ccatgtcagc cgaaagactt tgttggtcgc
      361 gaatatatac agaaagaagt ttgggattat cttgagaatg ttagaggaaa tagctctcaa
      421 agtagagtcc tatcacttat aggcggttca ggaaatggaa agtcatcttt aatagcaagg
      481 ctatcctcaa gatttaaaaa tcaaaaatgg aagaataaat tcttccttac accagtcgat
      541 gtccgttctg ctagaggcgg acgttttgtg gcagaagctg tcgttaaagc tttcagcagt
      601 gcgatcaaag aaggttttat cgaatatgaa aaaccattcc tgattgagag tgttacggat
      661 atcataggat ctgagagtgt tcaggaatgt ttaaaatacc tttccgaaca tgataaggta
      721 ttgactattt tctttgatca gttcgaagaa gtctttatga aagaggaact attcgggcta
      781 tttaaagaat ttgaacgatt cgcactcgat gtctcggcac tacaaagcaa tttagttgta
      841 gggttctcat ggagaactgg tattacttta ggggatgaaa atccagcgta ctcaatgtgg
      901 aatcggctaa aagattatag ggttgaaaag aaacttgagc cttttgatct aaaagattca
      961 tctaaattaa ttaatagttt cgagctaaat acagggttta aattaaataa acctttacgc
     1021 acgcgtctta ttcagcaagc tcaaggttat ccatggttat tgaaaaagct ttgtattcat
     1081 gtattcaaaa aactaaaagg aggtatctca caggatcaaa tgttggttag tcaactacag
     1141 atctcaaatt tatttcatga ggattta
//
LOCUS       DAPHJE010000302         1137 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPHJE010000302 DAPHJE010000000
VERSION     DAPHJE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.306"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..360
                     /locus_tag="RH222_004596"
                     /pseudo
     CDS             120..360
                     /locus_tag="RH222_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            452..667
                     /gene="sctI"
                     /locus_tag="RH222_004597"
     CDS             452..667
                     /gene="sctI"
                     /locus_tag="RH222_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV3799486.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            708..>1137
                     /gene="sctJ"
                     /locus_tag="RH222_004598"
     CDS             708..>1137
                     /gene="sctJ"
                     /locus_tag="RH222_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HDV3799487.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALP"
BASE COUNT          373 a          197 c          239 g          328 t
ORIGIN      
        1 atttaatgtg aatttgaaaa atcaggacac aaataatagt tcatttagct acggagataa
       61 ccgattcgag aagtctcaag gtagcaactg gacatttcag gaataattga ggaggactta
      121 tggcagattg gaatggttat attatggata tcagcaatca atttgatcag ggcgttgatg
      181 atctgaacca gcaagttgaa aaagcgttgg aggatttagc caaccaatcc ctccgacacg
      241 aaatttcttg ccgaatatca aagtgcatta gctgagtata cattatatcg aaatgcgcaa
      301 tctaacgttg taaaagcgta taaagatctt gattcagcaa tcatacaaaa cttccgctaa
      361 actggataag gattttatta tgtcagtaag caatatgcca cctattgata gagctgagta
      421 atcaactgct catgaaatac agcaagcgaa agtgattgat ttaaatgacc gggttctcaa
      481 tcttgacaac ccggatgata aaatgatctc cgcttttgcc aactatgctg tacaaactga
      541 aaattggcag caaaatgcgt tacaagcatt aaggtctgat aaagaggggt taactcctga
      601 aaaattatta gtcttgcagg atcatgtgct taattataat gtcgaggttt cattggtgga
      661 acattagcaa gaaagattgt cgctgcagtt gagacgttga cccgctcatg aaatatatat
      721 cattattatt gtttatcctg ctgttgtgcg gctgcaagca gcaggaatta ttgaaccatc
      781 ttgatcaaca gcaagctaat gatgtccttg ctgttttaca aagacataat attaatgctg
      841 aaaagaagga tcaaggcaaa acaggatttt caatatttgt cgaaccaacg gattttgctt
      901 ctgcggtgga ttggctaaaa atctacaatc taccgggtaa gcctgatatt caaatatcgc
      961 agatgttccc tgcagatgca ctcgtttcat ctccacgagc ggaaaaagca aggctatatt
     1021 ctgctattga gcagcggtta gagcaatctc tcaaaattat ggatggcatt atttcttcga
     1081 gagtacatgt tagttacgat gttgataatg gcgatagtgg taaaactgct ttaccaa
//
LOCUS       DAPHJE010000303         1119 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPHJE010000303 DAPHJE010000000
VERSION     DAPHJE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.307"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..824
                     /gene="gadX"
                     /locus_tag="RH222_004599"
     CDS             <1..824
                     /gene="gadX"
                     /locus_tag="RH222_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV3799488.1"
                     /translation="QSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVD
                     KCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQN
                     KNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTL
                     ARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYH
                     SVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          330 a          209 c          238 g          342 t
ORIGIN      
        1 tgcaatcatt acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca
       61 tggttaatgg tgaatatcgc tattttaatg gcggtgacct ggtttttgcg gatgcaagcc
      121 aaattcgagt agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat
      181 tatttctgcc gatgctcaag gaggaggcat taaatcttca tgcacataaa aaaatttctt
      241 cattactcgt tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat
      301 cgcagaataa gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt
      361 tgttatctgt ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac
      421 cgaacatgcg aacacgagtt tgtacggtta tcaataataa tatcgcccat gagtggacac
      481 tagcccgaat cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg
      541 aagaagagac atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac
      601 ttattgttat acatggcttt tcaattaagc gagtcgcagt gtcctgtgga tatcacagcg
      661 tgtcgtattt catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg
      721 agcgatcggc gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa
      781 atttttacgg cactgaccgt tctgcggaag gaataagatt atagaatttt actcagacat
      841 aaaaaaaacc cggcataggg gaccgggaag aggatagtct gccgtctcca gactaataaa
      901 ccgttataac actccctgtt ggcacgggaa actttgtgct ctcagtaagt taaatataac
      961 ttttactgga aataagatca gccatttttt tataaacata agctatacgc agtgcgaaaa
     1021 tatattcgtg ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat
     1081 aaattttggt cctaccaaat ctggcagttt tttcgctaa
//
LOCUS       DAPHJE010000304         1116 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPHJE010000304 DAPHJE010000000
VERSION     DAPHJE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.308"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..281)
                     /locus_tag="RH222_004600"
     CDS             complement(<1..281)
                     /locus_tag="RH222_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799489.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAA"
     gene            complement(988..>1116)
                     /locus_tag="RH222_004601"
     CDS             complement(988..>1116)
                     /locus_tag="RH222_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3799490.1"
                     /translation="NLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          324 a          235 c          192 g          365 t
ORIGIN      
        1 gcggcaagtt ggagtggatt tagatttttc agatatggtc ttatgatctt tcccttactt
       61 aataaatagc atgtatagga ttcggaaagt ccagcatcta ttttgtacct cattatatta
      121 gataaggatg tgagaaattc cttgttatca ttcgcagcca tatatgccag tccgagttca
      181 gcctctgcgg ccttacaggc aaaatagctt agtgaacacg cttttcgtcc ctctttagta
      241 ctggctgata ttagtgccca taatattgct ttgtctgcca aatctaactc tctctgaaat
      301 tgtcttgcag taactgcact cgcagaaata gagaaaaaaa tacaattgtt aataaccttg
      361 ccatattttt tagtccgtaa ctgttaatgt gctatctggc tctgaaaata ttaactcatt
      421 taatttttgc atgtcattag ataaaccagt tatacaaccc tcggaacaga atcgaggtcc
      481 taaagacgga ccaaaagcgt ttaaacaact atgtattgaa aatccattgc gattataagt
      541 tcctgtatta actgacggta ctaatcgtat gccgtcaggt tttctattgc ttgctcccgg
      601 gtcattaaca ttccaactcc ataggccttg aggtattggc catctattta taaatgtgta
      661 caacggggat tatttttaca ttgcatatta ttaccagaag cgactggaat actaagcaca
      721 tcataaaaat tatcatcaca tggtatatct gaaacacacg aaagcatacc taaagttata
      781 ctgtataaac aggtggctaa tcccaagggg atcgattcca gaaatcgggt tcaacggata
      841 ctgataaaaa ttccacctac cttccagccc aatcggagcc tgagtgttat accgcccctg
      901 ctgcggttaa tgttaccgat aatagttata gtacagccct gattcatcat catactactg
      961 ctgcggcagt ctgatgaact attgctttta cggattctcc ttattcttcc ttctatccat
     1021 tcgctttagc tttttgagct cttgaagttt gctctttgca tcaatattac cgttatttac
     1081 agcgaattcc atccaataaa tagctctatt caaatt
//
LOCUS       DAPHJE010000305         1111 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPHJE010000305 DAPHJE010000000
VERSION     DAPHJE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.309"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..657
                     /locus_tag="RH222_004602"
     CDS             <1..657
                     /locus_tag="RH222_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3799491.1"
                     /translation="VPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVAL
                     LVRFEKLLESDAPLAEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMF
                     RKEYFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWG
                     GAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL
                     "
     gene            654..>1111
                     /locus_tag="RH222_004603"
     CDS             654..>1111
                     /locus_tag="RH222_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3799492.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSIT"
BASE COUNT          250 a          271 c          312 g          278 t
ORIGIN      
        1 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
       61 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa actgcagcaa
      121 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
      181 gtgctgcctt ccatctacaa acagcatgaa gagcgctaca ccggttatac cctgcgtcag
      241 ttgtgtcagg aaatgcacga tttgtatgcc cgccacaacg tgaaacaact gcaaaaagag
      301 atgttccgta aggaatactt cccacgcgtc agcatgaatc cacaggaagc caactacgcc
      361 tatttacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc
      421 gagggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga gatctggggt
      481 ggtgctgttc tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
      541 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtctggtgc
      601 tatgtcatca agccccgtga tgcgcaaagc accctgttaa aaggggaaaa attatgagtc
      661 aggctaaatc gaacaagatg ggcgttgttc agttaaccat actgacgatg gtcaacatga
      721 tgggctctgg catcatcatg ctgccgacta aactggcaga ggtcgggacg atctcgatta
      781 tctcctggct ggttacagct gtaggctcaa tggcgctggc atgggcattc gcaaaatgcg
      841 gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct ttcggtaaat
      901 ccggtaactt tatggcgaac tatacctacg gcgtctcact gctgattgct aacgtcgcga
      961 ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc
     1021 agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac tttggtggtg
     1081 cgcgcattac cgggcaaatc agtagcatta c
//
LOCUS       DAPHJE010000306         1111 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPHJE010000306 DAPHJE010000000
VERSION     DAPHJE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.310"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..657
                     /locus_tag="RH222_004604"
     CDS             <1..657
                     /locus_tag="RH222_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3799493.1"
                     /translation="VPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVAL
                     LVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMF
                     RKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWG
                     GAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL
                     "
     gene            654..>1111
                     /locus_tag="RH222_004605"
     CDS             654..>1111
                     /locus_tag="RH222_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3799494.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSIT"
BASE COUNT          255 a          267 c          307 g          282 t
ORIGIN      
        1 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
       61 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa gcttcagcaa
      121 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
      181 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac cctgcgtcag
      241 ttgtgtcaag aaatgcatga tttgtatgcc cgtcacaacg tgaaacaact gcaaaaagag
      301 atgttccgta aggaacactt cccacgcgta agcatgaatc cgcaagaagc caactacgcc
      361 tatttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg tatcgctgcc
      421 gaaggtgcgc ttccttatcc tccgggtgtg ctgtgcgttg ttccgggtga aatctggggt
      481 ggtgctgttc tgcgttactt cagcgctctg gaagaaggga tcaacctgtt gccaggtttt
      541 gcaccggagc ttcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc
      601 tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa attatgagtc
      661 aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg gtcaacatga
      721 tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca atttcaatta
      781 tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc gcaaaatgcg
      841 gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct ttcggtaagt
      901 ccggcaattt tatggcgaac tacacctacg gtgtctcgct gctgattgct aacgtcgcga
      961 ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg tcgccagtgc
     1021 agattggtct tgcgaccatc ggagtgctgt ggatttgtac cgtggctaac tttggtggtg
     1081 cgcgcattac cgggcaaatc agtagcatta c
//
LOCUS       DAPHJE010000307         1093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPHJE010000307 DAPHJE010000000
VERSION     DAPHJE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.311"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..933
                     /locus_tag="RH222_004606"
     CDS             <1..933
                     /locus_tag="RH222_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV3799495.1"
                     /translation="EPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGIVNAAK
                     VLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESILGALDI
                     EGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQFQTDL
                     IIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPEEQNFS
                     GLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLEKLPFP
                     SLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
BASE COUNT          312 a          269 c          214 g          298 t
ORIGIN      
        1 gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac aaccttacga
       61 aatattgatc ttaaccttct gactattttt gaagctgtat atgtacataa agggatcgtt
      121 aatgcagcga aagtgcttaa tctgaccccc tcggcaatca gtcagtctat tcagaaactg
      181 cgcgttatat tccctgaccc attgtttatt cgcaaaggcc agggtgtcac tcctaccgca
      241 tttgcgatgc atctacatga gtatatcagt cagggccttg agtccattct tggcgcgctg
      301 gatatcgaag gaagctatga taagcaacga acgataacta ttgcgaccac tccctcggtc
      361 ggagccctgg tccttcctgt catctatcgg gcgattaaaa ctcactatcc gcagctttta
      421 ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca gtcagtttca aaccgatctc
      481 atcatcgata acatgttttg caccaatcgt acggtgcaac atcatgttct gttcaccgac
      541 aatatggtgt taatttgccg cgagggaaat ccactactct ctttagaaga tgacagagag
      601 actatcgaca acgctgcgca tgtacttctg ttaccggaag agcaaaattt cagcggtctg
      661 cggcagagag ttcaagaaat gtttccggac cggcaaatca atttcaccag ctacaacatt
      721 ttgacaatcg ctgcactggt tgccaacagt gacatgttag cgattattcc cagccgtttt
      781 tataacctgt ttagccgctg ctggccgctg gaaaaactgc cttttccgtc cttaaatgag
      841 gagcaaatag atttctctat ccactacaac aaattcagcc tgcgtgatcc catcctgcac
      901 ggagtaatag atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag
      961 cgcagaacga taaaatggca caatccaaca acaattttac attttcgcga ccgctttggc
     1021 tgctttcagg tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat
     1081 tacaacaatc tat
//
LOCUS       DAPHJE010000308          970 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPHJE010000308 DAPHJE010000000
VERSION     DAPHJE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.312"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="RH222_004607"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="RH222_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV3799496.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          263 a          236 c          214 g          257 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccag tagttcatcc agtgcttcaa tatcgcgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacttcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggcagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg
      601 cgactaatga cagccaacaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaaaa tgcgaaacat atcgttaata
      961 attaaaggga
//
LOCUS       DAPHJE010000309          970 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPHJE010000309 DAPHJE010000000
VERSION     DAPHJE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.313"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(197..352)
                     /locus_tag="RH222_004608"
     CDS             complement(197..352)
                     /locus_tag="RH222_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799497.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(432..497)
                     /locus_tag="RH222_004609"
     CDS             complement(432..497)
                     /locus_tag="RH222_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799498.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(500..688)
                     /locus_tag="RH222_004610"
     CDS             complement(500..688)
                     /locus_tag="RH222_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV3799499.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(699..911)
                     /gene="cspI"
                     /locus_tag="RH222_004611"
     CDS             complement(699..911)
                     /gene="cspI"
                     /locus_tag="RH222_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HDV3799500.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          312 a          191 c          167 g          300 t
ORIGIN      
        1 ttgtttttaa gttttcaata ttcatcattt cctcttttag tctgttatga ctttccagtt
       61 acacagtaag tcgattatat ggtgcaaacg tgtaaaagat aagatgaaac atcgcaataa
      121 tcaacatacg atagtctaaa ttttacacaa acagacaaag agaattttcc tgaattatca
      181 atacaatagc atcaaatcaa ctcaagagcc ttattgctgc ttccagaatt tcttctgaag
      241 taacatgtcg atccgcggct acataaatga ctttatgatc tccggtcaga gatggaaacc
      301 ctgcggccat tacagtaagg tgtgtttttt cgccatttgg atattcacgc atgatggtgt
      361 taactccagt catcgctggc actaccactg ctggttcaga gttaaaaaaa actatgattt
      421 ttttcatgat gttaccgtag tatgtgagta tccatcgaat agataccaag caaaaaagct
      481 cccgaaggag ccttcatttt cactttttta aatccaacga cagacggctg gcatttaagt
      541 attgtgaaat attatcaaat gtaatcatca ttgatttaca aaagatacat tttgccccga
      601 aaggattcat gtcagaaaca tcaaaagatg atgttctata ctgggaacca tgacaacacg
      661 ggcatctaaa gtgaatatgg tttgtaatat tgtctacctc aaagcgccac tacatgaaca
      721 gcggcaggac ctttaggtcc gttctcaata ccaaattcaa cttcctgatt ctcagttaat
      781 gttttgaaat cgttgctctg aattgctgag aaatggacaa acacatcttt gctgccatct
      841 ttcggcgtga tgaaaccaaa acctttttca gggttaaacc atttcactaa accagtcatt
      901 ttgttagaca taattattac cttttgaaga aattagccct tgggcagaat ggtccgaaaa
      961 aaaatatcag
//
LOCUS       DAPHJE010000310          954 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPHJE010000310 DAPHJE010000000
VERSION     DAPHJE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.314"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            291..845
                     /locus_tag="RH222_004612"
                     /pseudo
     CDS             291..845
                     /locus_tag="RH222_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          315 a          165 c          238 g          236 t
ORIGIN      
        1 ttatgaagaa aaatgaaaca tggatgttat taaatggaga tgcaacacca aacattagaa
       61 caatgagcaa taagaaaatt gaatgcctgg agaagagtga agtagaatgg gctagtaggg
      121 tttgcgacag tatcacagac aaggaaaggg aaaatctgaa ggcattaata tgtagcaaac
      181 cagctaaatc agttaataca ggcaaaagac cagcgttagg atattgaaac cggcactgca
      241 ctatatcact attacaatat tctgagtgac tagagcaact gctccgccat ccgggctagc
      301 agtatgacga cgaatccgaa ctgcactaca actggcagcg gtactacaat ccgggacagg
      361 gacgatatat cacccaggac ccgatagggc tggcgggggg attaaacccg tacagttatg
      421 ttatgaacaa tcctgtgcaa tggattgatc cattgggatt agctcatact tcaggaaaat
      481 ggaaggactg tggtaaaggt tgtcgaatcc gaatagatgg cgatgctgtt ggtgacggaa
      541 gacatttgca ttgggagtgt aaaaatggga gtggtgtaat gggggaattt ggaggtatgt
      601 cccatggtga ggattactca tcagcaccaa atagcattaa gaaatgtgca aaaaaatatg
      661 gtttcgagcc agaaccaagt aaagatctgt catgcaaaac ctccgggcta tcaactactg
      721 atgcggctct tgcaactggt gctacaattg gcgcagggta tgttatatat agagtgattc
      781 gatttatacc ttcacttgca ccacctcttt ggtggtcaat acctgcaaac gttgttactc
      841 catgattaaa acggataatg gagattatat aatgagcgat tttgacattg tgagttcaat
      901 tgactggcat gatggggttt tgttagaagt aaaaacgaca ttcaaagaat ctca
//
LOCUS       DAPHJE010000311          950 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPHJE010000311 DAPHJE010000000
VERSION     DAPHJE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.315"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            214..>950
                     /locus_tag="RH222_004613"
     CDS             214..>950
                     /locus_tag="RH222_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV3799501.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQ"
BASE COUNT          268 a          175 c          231 g          276 t
ORIGIN      
        1 gcaacaataa ctcatagttc atgtttaaat aaaataagaa ctatgcaatt tttaatctat
       61 caattcgaaa gcattagcaa attagccaaa ttgacacctt cccacctcca actaacactc
      121 aatacagacc tctgacaatt taataagttt catttttaat gtctgacgac tcatctcttt
      181 cgtggctgta ttgtttgtat atggagcgtt tatatgaata gaatctatcg cgtgatatgg
      241 aattgcactc tacaggtatt tcaggcctgc tcggaattaa ctcgcagggc aggtaaaaca
      301 tcgacggtta atttgcgtaa atcctctgga ctgacaacga aattcagtag attgacgctg
      361 ggtgttttgc tggcactaag cggttcagcg tctggtgcaa gtctggaagt tgataatgat
      421 cagattacca atattgatac tgatgttgct tatgatgcct acctggttgg ctggtatggc
      481 actggagtgc ttaatatttt ggctggcggt aatgcctcct taaccactat tactaccagc
      541 gtcattggcg ctaatgagga ctcagagggc accgttaatg ttttgggtgg cacctggcga
      601 ttgtatgata gcggaaataa tgcaaggcct ttaaatgtgg gtcaatccgg aacggggacg
      661 ctgaatatta aacagaaggg tcacgtcgat ggaggctatt taagattagg ttcttcgaca
      721 ggaggcgtcg ggacggtcaa tgttgaggga gaggactctg ttctgacgac cgaattattc
      781 gaaataggga gttacggtac gggctcatta aatattacgg ataagggtta cgtcacgagt
      841 tcaatcgtcg ccattttagg ctatcaggcg ggcagtaatg gccaggttgt cgttgaaaag
      901 ggtggcgagt ggctaataaa aaataacgat tcctcaattg aatttcaaat
//
LOCUS       DAPHJE010000312          922 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPHJE010000312 DAPHJE010000000
VERSION     DAPHJE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.316"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          274 a          165 c          190 g          293 t
ORIGIN      
        1 gatgtttaat tgatttgaat gttcgtaagc tatatcactt actcaatcca ttttacccag
       61 agtcataaaa tagaatgtct gttgatctca ttttcatatt cctttcagac tgaagtgcgt
      121 tttcatcacc cgatgagaaa gaactgaagt gatgtttgcc atagattgcg agatgccctg
      181 tctcattgac tatcttggtg cacctttata cctgttatac cagatcaaaa atcacgcaat
      241 ccatacaaca aaaccagatt tgcaattcgt gtcacaaaat atgtcgatct ttttctaaga
      301 ggaagatgcc atgtgaagcc agacgaacac ttgcggtggt cttcaaaaac taaagatctt
      361 agtttaacta tttgttttat aaataatttg tttagagtct aaacaagggg agctttgcaa
      421 gctaactcag tgagcttggt gaaaatcagt gtttacccgc catcaggctg agcataattc
      481 tcatcatgaa atatgtttcc tggtttgtgg cttgtaactg gtcacttctg aagtcgatct
      541 ggagaggctt gttgatgttg gtgttttcag gatgatgttt cacttagttt gtttgccgta
      601 tcgcccggcg aatggctgtg attgaggaag gtttaagtcg tagtgaccaa agctatattt
      661 accaacgaat gtagatgaaa aaatcatctc ctgcgttccc ccatatctcc aggataaaaa
      721 ggaatgtaac aatctcatgg cgtaagctga cgaatcagca ggaataatcg ctagggacct
      781 aagaattagc atgataatag ccactaagaa attactgcgc tccatgaaat agccattttg
      841 tggcaaatgg agttgactaa taatgtcata tgtgagacgg ctagttgaac gaatattaaa
      901 ttttgctgaa ttttttatgt tg
//
LOCUS       DAPHJE010000313          916 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPHJE010000313 DAPHJE010000000
VERSION     DAPHJE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.317"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            218..790
                     /locus_tag="RH222_004614"
     CDS             218..790
                     /locus_tag="RH222_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV3799502.1"
                     /translation="MASATSENTSSTKNEAQTVLSERSLKAEQRGMEIMDDMQYARLA
                     LFSGDTGAADKLINRAEDLLKSDDKTWETVVRKDKKTPQEGDVYIIINSSLMLSEDFT
                     VTPEKQKAIDEANKKLDKGDKKGAIDVLHLAGIDISETQWLMPLKQTQKKVNSAQKLM
                     SEGKYYEANLALKGAEDGVITDTVSLNEGD"
BASE COUNT          315 a          164 c          210 g          227 t
ORIGIN      
        1 gcagaacata aaatatgcat gccggatagg ggaatataaa tgtccgggag ggggacatcc
       61 ttgtccggct tcctgagtat atgactaatc gtcaggggga aaatcatatt atgtttatta
      121 tcaggtatga ttctttctgc ctgatgtcgg agtaaaaaat atgaaacata tctttaatgc
      181 tgtagcaatc gctctgtccc tgacctctgc tgtcgcaatg gcctcagcaa cttcagaaaa
      241 cacttcatcc acaaaaaatg aggctcagac agttttatca gaaaggagtc tgaaggcaga
      301 acagcgtggt atggaaatta tggatgacat gcagtatgca cgtcttgcct tattcagtgg
      361 tgacacagga gcagcagata agctgataaa ccgggctgaa gatttgctta aatctgatga
      421 taaaacatgg gaaacagttg tccgcaagga taaaaaaaca ccacaggaag gcgatgtcta
      481 cattattatt aacagcagct taatgttgtc tgaagacttt actgtcaccc cggaaaaaca
      541 gaaagctatt gatgaggcca ataaaaaact ggataaaggt gacaaaaaag gagcaattga
      601 cgtactgcat ctcgccggaa ttgatatttc agaaacgcag tggctgatgc cattaaaaca
      661 gacgcagaaa aaggttaaca gtgcgcaaaa attaatgagc gaagggaaat actacgaagc
      721 caatcttgca ctgaaaggtg cagaagatgg tgtgattacg gatactgtca gcctgaatga
      781 aggtgactga taacatttat atgcgcgtat aaccacggtg accggactgt attttgtccg
      841 gttactttaa taataaaacc gaacttctta atataatgtc aggaagtaag tctgtcagct
      901 ataataagaa aaatac
//
LOCUS       DAPHJE010000314          916 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPHJE010000314 DAPHJE010000000
VERSION     DAPHJE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.318"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            314..898
                     /locus_tag="RH222_004615"
     CDS             314..898
                     /locus_tag="RH222_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799503.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          282 a          181 c          170 g          283 t
ORIGIN      
        1 taataaaaat tttagtttaa acatttttgc agaaacataa tataaattcc cgcagataaa
       61 tatcaatccc atcgccatac cctaaccaat tgcacttgtg aatttatttt tgcttaattt
      121 tgaattaaga aaatttaatt tgttcaaaag cccttaaagt gcacaatagg cgtcctaatg
      181 aacaatggtt gtaaattttg gtaacaaata tcattgctca ttaatatcta gcatctgctt
      241 ataacatttg tgtcatgaat agtcattctt caaaccagta gtctgactaa tggaatttta
      301 ttcaaggatt tttatgtcta aaaaattagg ttttgccctg tctggtctta tgctggcaat
      361 ggttgcaggt actgcttctg ctgatatgga tggtggtcag ttaaatatca gtggtctggt
      421 tgttgataac acctgtgaaa cccgcgttga cggcggtaat aaagatggtc tgatcctgct
      481 gcaaactgca actgttggtg aaatcgatgc tggcgtgctg aatgacaccg ttggcgctaa
      541 agccaaacct ttcagcatca ccgttgactg cagcaaagca aatccgaatc caggtagtac
      601 agctaaaatg accttcggtt ctgttttctt tggtaatagc aaaggaacgc tgaacaacga
      661 catgtctatt aacaacccgt ctgacggcgt gaatatcgct ctgcacaata ttgatggttc
      721 cactatcaaa caggttcaaa ttaacaaccc tggcgatgtg tatactaaag ccctggatgc
      781 aacgacaaaa tctgctgttt atgattttaa agcgtcttac gttcgtgccg tcgcagacca
      841 aacagcaact gctggttatg taaaaactaa cactgcatac accattactt atcagtaagt
      901 attcaccaat taaatt
//
LOCUS       DAPHJE010000315          898 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPHJE010000315 DAPHJE010000000
VERSION     DAPHJE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.319"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            41..826
                     /locus_tag="RH222_004616"
     CDS             41..826
                     /locus_tag="RH222_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3799504.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          295 a          182 c          192 g          229 t
ORIGIN      
        1 aatattctaa tcattatgac aggcgaggga gtgtccaatt atgaattcaa aaaagctttg
       61 ttgcatatgt gtgttattct cgctgcttgc aggatgtgcc tctgaatctt ctattgatga
      121 aaagaagaaa aaagctcaag tcacacaaag taatattaat aaaaacactc cccagcaact
      181 gacagacaaa gatttattcg gtaatgaaac cactctggcc gtatccgaag aggatattca
      241 agctgcgctt gatggagatg agttccgcgt tccccttaat tctcctgtaa tacttgttca
      301 atccggcaac cgcgcaccgg aaaccattat gcaggaggag atgcgaaaat attatactgt
      361 ttccacattc tctggtatcc cggacaggca aaagcctctg acttgtaaca aaaacaaaga
      421 taaaaatgag aacgaggatg ttgccagtgc tgagaatatg aactggatgc aggcactgcg
      481 ttttgtggct gcaaaaggac atcagaaagc gattattgtt taccaggata cattgcagac
      541 aggaaaatat gactctgcgc tgaaatcaac agtatggtcc gactataaaa atgaaaaact
      601 caccgacgct atctccctgc gctacctggt acgtttcacg ctggtggatg tggcaacagg
      661 tgagtgggct acctggtcgc cggtgaatta tgaatataaa gtgcttccac cattgcccga
      721 caagaatgaa gccagtacga ctgatatgac agagcagcaa atcatgcaac ttaagcaaaa
      781 gacctataaa gcgatggtaa aagatttggt taaccgctat caataataaa ttatatctgc
      841 cgccaggaat ttctggcggc aataatacaa aattctttgc ataagaaatt tttcttga
//
LOCUS       DAPHJE010000316          894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPHJE010000316 DAPHJE010000000
VERSION     DAPHJE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.320"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>894
                     /locus_tag="RH222_004617"
     CDS             <1..>894
                     /locus_tag="RH222_004617"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775087.1"
                     /note="Wzc; catalyzes the autophosphorylation on tyrosine
                     residues which downregulates the biosynthesis of colonic
                     acid (an extracellular polysaccharide); Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV3799505.1"
                     /translation="LGRLLGELIDHRKLIISVTSLFTLLALIYALFATPVYQADALVQ
                     VEQKQANAILSNLSQLLPDSQPQSAAEIALLQSRMILGKTVDDLNLQTRIEPLYFPLF
                     GKGWARLTNKNAGSLDVVKFYLSGANDEDAPDAEVRVIDKNHFEITVNDNVYKGTVGE
                     LLQNNDVIIKVDRINSEAGNEFKLTYYSRYKAIANLQENLSVIDKGKDTGMLTLSLSG
                     YDPVLIQKIVDNIASNYLAQNIARQAAQDAKSLDFLNEQLPKVRSDLDLAESKLNEYR
                     KKSDSVDLTLEAKAVLDQIVNV"
BASE COUNT          316 a          129 c          179 g          270 t
ORIGIN      
        1 ttaggtcgcc ttttaggtga attaattgat cacagaaagc ttattatatc tgtgacctcg
       61 ctttttactt tattagcact aatttatgct ttgtttgcaa ctcctgtata tcaagctgat
      121 gcgctagtgc aagttgagca gaaacaagcg aatgctattc ttagtaatct aagtcaattg
      181 ttacctgata gtcaaccgca atcggcagct gaaatagctt tattacaatc aagaatgatt
      241 ttgggcaaaa cagttgatga tttaaattta caaacacgaa ttgagccact ttatttccca
      301 ttatttggga agggatgggc tcgcttgacg aacaaaaatg ctggtagttt ggatgtagtc
      361 aagttttatc tatcgggcgc gaatgatgaa gatgcacctg atgcagaagt tagagtaatt
      421 gataaaaatc acttcgagat tacagttaat gacaatgtat ataaaggaac agtaggggag
      481 ttattacaaa acaatgatgt aattataaaa gtagatagaa taaattcaga agcgggtaat
      541 gaatttaaac ttacatacta tagtcgctat aaagcgatag ctaatttaca agaaaacctt
      601 tcggtaattg ataaaggtaa agatactgga atgctaactc tctcactgag tggttacgat
      661 ccagtattga ttcaaaaaat agttgataac attgcatcca attatttagc tcaaaatata
      721 gcaaggcaag cagcgcaaga tgcgaaaagt ttagattttc tgaatgaaca attgccaaaa
      781 gtaagaagtg atctcgactt agctgaaagt aagttaaatg aatataggaa aaaaagtgat
      841 tctgtagacc ttacacttga ggcgaaggct gtattagatc aaattgttaa tgtt
//
LOCUS       DAPHJE010000317          886 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPHJE010000317 DAPHJE010000000
VERSION     DAPHJE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.321"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(254..591)
                     /locus_tag="RH222_004618"
                     /pseudo
     CDS             complement(254..591)
                     /locus_tag="RH222_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604131.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(569..865)
                     /locus_tag="RH222_004619"
     CDS             complement(569..865)
                     /locus_tag="RH222_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HDV3799506.1"
                     /translation="MGAVFSYSEPEEPPPLNFSFEERKKVFLWVLTRLLKEGRIKLAK
                     HGKFLEGSVEEQVERFRQAFPKTEEEMEDGIWFFDESCPGGAVWGLEDGSLEWT"
BASE COUNT          224 a          252 c          171 g          239 t
ORIGIN      
        1 attatctaaa caaaaatttt cgccaactta aagttgtaag gcatatttta aaaatatgtt
       61 aaccaattgg agcgttattc tatcctcccc gcggcaatta ctctgtcctt tttgtaatcc
      121 gagtattata tgtataattt catacaaagt atgggtgtgt attatcgtga aggagatcgg
      181 tggggataac ctccattgaa attggagtta ctcgccgatc actttaacaa taatacgcat
      241 ctgtggtatc attccccacc acctcgccgg aggcaacacc tgcagcaccg gtaagggtat
      301 tatttccctg tgccaccgcc agtacagcat tcaccacggc atgggctacc acacgccccg
      361 caccgtccgg gggtctgttt gataatttca gcaatatacg gcgcagcacc atctgccaag
      421 gctgcactca gattaccgcc agccagtccc tgtaccactg ccgttgcagc ctgtatcgcc
      481 tgctggtatt gccccccatc ccaaaaccgg agccgccata ttgttgttat acgctcgctg
      541 cgccgcatcc tgcgctgtga agggtttatc aggtccattc gagagagcca tcttcaagcc
      601 cccaaacagc tccccccgga caactctcat caaaaaacca gatgccgtct tccatctctt
      661 cttctgtttt cggaaatgcc tgtcggaaac gctcaacctg ctcttcaaca cttccttcca
      721 gaaacttacc gtgcttggcg agtttgatac gcccttcttt cagaagacgg gtcaataccc
      781 ataaaaaaac ttttttcctt tcttcaaatg aaaaatttaa cggcgggggt tcttctggtt
      841 cactatagga aaaaacagcc cccaaattac gaccttctgc agcttc
//
LOCUS       DAPHJE010000318          873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPHJE010000318 DAPHJE010000000
VERSION     DAPHJE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.322"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            253..855
                     /gene="fimB"
                     /locus_tag="RH222_004620"
     CDS             253..855
                     /gene="fimB"
                     /locus_tag="RH222_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV3799507.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          256 a          163 c          195 g          259 t
ORIGIN      
        1 agggttatta ttagattcgt atccgattga taaatatata aaggtacata gcatgcaaga
       61 gcatggcgtt tgtatggcaa cgttattata attaacagtt gctactccat ttaagttcac
      121 tcagaagaac tggtccactt acgttagtta ttaagcaaac gttcgctttt ataaacataa
      181 tcaggataaa aatgttggat tattgctaac ccagcacagc tagtgcgcgt ctgtaattat
      241 aagggaaaaa cgatgaagaa taaggctgat aacaaaaaaa ggaacttcct gacccatagt
      301 gaaatcgaat cactccttaa agcagcaaat accgggcctc atgcagcacg taattattgt
      361 ctgactttgc tttgttttat tcatggtttc cgggcgagtg aaatttgtcg attgaggatt
      421 tcggatattg atcttaaggc aaagtgtata tatatccatc gattaaaaaa aggcttttca
      481 acaacgcacc cgctattgaa taaagaagtt caggctttaa aaaactggtt gagtatccgt
      541 acttcgtacc cgcatgctga gagcgagtgg gtatttttat cacgtaaggg gaatccgctt
      601 tctcggcaac agttttacca tattatctcg acttccggtg gtaatgccgg gttgtcactg
      661 gagattcatc cgcacatgtt acgccattcg tgtggttttg ctttggcgaa tatgggaata
      721 gatacgcgac ttatccagga ttatcttggg catcgcaata ttcgtcatac tgtctggtat
      781 accgccagca atgcagggcg tttttacggc atctgggata gagccagagg acgacagcgt
      841 cacgctgttt tatagagata gttgagatac cag
//
LOCUS       DAPHJE010000319          863 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPHJE010000319 DAPHJE010000000
VERSION     DAPHJE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.323"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            306..>863
                     /locus_tag="RH222_004621"
     CDS             306..>863
                     /locus_tag="RH222_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799508.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFS"
BASE COUNT          288 a          138 c          164 g          273 t
ORIGIN      
        1 ataaaaaagg tgtcccaggg cttagttgtt ttcaaccaca ttaaaactcc atgagtgctg
       61 tggtatatct ttttaatcct gccagtattt cataggtttt cttaatcgat tgtagaggca
      121 tgttctgatg acgcctctac aatctttagg gactttctct gctatgagat aagaaatttc
      181 atattatgct ttaaggctga atgatttgtg caaaatataa ttatttttcc atcgaacatg
      241 aggtgtatgt cataagatta aatgttaact atgcattcct cgcggtgaat tatatgaggt
      301 aaaagatgaa aattggaact gtggcaggca ctaacgacag tacaacgaca atcgcaacaa
      361 atgatatggt gcaagagcat gttactaact tcactaaaga actgtttggg tatattgcaa
      421 atggtatagg tgatgatata tcaagtattg ccaggacaat gctcggtgaa gttgtggaaa
      481 aaattgatga ttggcaaata gaacgtttcc aacaatctat tcgagatgac aaaatatcat
      541 ttaccataca aacgaatcat tcagaaaaat atagtatgct atcggacatg cgcgctcata
      601 ttctacgtag ggataataat tatcagttta ttgtgacaat taatagtaaa aattacggct
      661 gctatcttga taataccgat attaattggt gcagcattgt ttatttactt aataatatga
      721 cagtaaatga tgatgctaat gatgttgcag ttactgagtc gtacaaaccc gtctgggact
      781 ggaaaatatc gcaattcaat gtatctgata ttaaattcga aacaatgata aacccccagt
      841 tcgctgacag aacctatttc tcc
//
LOCUS       DAPHJE010000320          857 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPHJE010000320 DAPHJE010000000
VERSION     DAPHJE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.324"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>857
                     /locus_tag="RH222_004622"
     CDS             <1..>857
                     /locus_tag="RH222_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799509.1"
                     /translation="RRKFISFLSFSALYSSSRLSWGSDLYHINSMSELRNFKPEYIGQ
                     EVYLESYYVGVGRGGGYFTFHEGVRNDNGGTVISAVGGGYWLRSLHNDLCLVYDFGAR
                     DDIDGFDSTNAIQGAIDNCNHISLGGGHFNISHPLYLRSNIIFDGDFGSLSSLAPKNS
                     AFMQGSIFAPGNYHPDFLQKIIKKQILDCKDNSIKLDDVSGFRKGDIVRISSHESTLS
                     FGFWVENYIQLSRIIKVDSLNKTLIIEHPISFHGELELCLASTNSVKARFGRPLFCCS
                     DTIVRNTEV"
BASE COUNT          243 a          133 c          182 g          299 t
ORIGIN      
        1 tcgaagaaaa tttatttctt ttttgtcttt ttcggcattg tattcttctt ctcgattgag
       61 ttggggaagt gatttatatc atataaactc tatgtctgaa ttacgtaatt ttaaaccgga
      121 gtatatcggg caggaggttt atctcgagtc ctactacgtt ggagttggca gaggaggagg
      181 ctattttaca ttccacgaag gtgttcgtaa tgataatggt ggtacagtta taagcgcagt
      241 tggaggaggc tactggcttc gaagtttaca taacgatctt tgtttggttt acgattttgg
      301 tgcacgagat gacatagatg gttttgatag cacaaatgct attcagggag cgatagataa
      361 ttgtaatcat ataagcttgg gagggggcca tttcaatata tcacatccgc tataccttcg
      421 gtcaaatatt atttttgatg gcgacttcgg ttcactttct tcattagcac ctaaaaattc
      481 tgcgtttatg caaggttcta tatttgcgcc agggaactac catccggatt ttttacagaa
      541 gatcataaaa aaacaaattc ttgattgtaa agataattca attaaacttg atgatgttag
      601 cggttttaga aaaggtgata tagtaagaat atcttctcat gagtcgacat tgagtttcgg
      661 cttttgggtt gaaaactata ttcagctttc aagaataatc aaagttgatt ctttgaataa
      721 aaccttgata attgagcatc ctatttcttt tcatggtgag cttgagttat gtctcgcatc
      781 tacgaactcg gtaaaagcaa gattcgggcg tccattattt tgttgctcag atacaattgt
      841 ccgtaacact gaagtta
//
LOCUS       DAPHJE010000321          832 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPHJE010000321 DAPHJE010000000
VERSION     DAPHJE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.325"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          217 a          162 c          176 g          277 t
ORIGIN      
        1 attgagtaat cttatttcat agaaaaatat taatgacgtc ctgtggcgta tgcatgatgc
       61 aaggttgaac gcatgtaata tgatatttat cacgtaaatg cagtgtaatc ttcttgcatt
      121 gtttttaggt tgctattccg tttatctgag gactgctggc tattgttgtg cagttttcct
      181 gatacagctt tatgcaggtt ttccagcatt acagtaaaac ccgggaacat cagtgctgct
      241 cgtttctttc tcctgtatcc actcccgctt atagatactt ccttaaatat tctctcgtct
      301 gactaaagca tatatacccg ttcatgggat gttggctgta atgattttcc tgagcggatt
      361 attactgatg accataagta tgtctcagca cagtcatcgt aacgtgctgg ttaacctggc
      421 catggtagcc aaatcatcat cctgtatatt ctgttgaggg aggcactggt gattgttcag
      481 aatacggctg atgcgaaaaa gtgcaggctg tttcctttat tggtgtgatg tacagcctct
      541 ttacgctcta cttttccggg gaagaagcca tgctgtagca ctttattgtt acttttcctg
      601 gctggacact gtatggtctg gtatcccccc acgttttgaa cggaaaaaac agatttaaag
      661 ttgccagtag tctgtaagtt tctgcatccc atttccccac agacagtatt ctcctgcaat
      721 gtgatgaatg gtttcctgga acaggaaagg aactaagtca gatagtggag aaagtggtta
      781 acacgttgtt tttaattaaa aatttggtaa taacaataac ctgcataaaa ca
//
LOCUS       DAPHJE010000322          831 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPHJE010000322 DAPHJE010000000
VERSION     DAPHJE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.326"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            4..765
                     /gene="ydeO"
                     /locus_tag="RH222_004623"
     CDS             4..765
                     /gene="ydeO"
                     /locus_tag="RH222_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV3799510.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          253 a          166 c          165 g          247 t
ORIGIN      
        1 aaaatgtcgc tcgtttgttc tgttatattt attcatcatg ccttcaacgc taacatttta
       61 gataaagatt acgccttctc tgacggcgag atcctgatgg tagataacgc tgttcgtacg
      121 cattttgaac cttatgagcg gcattttaaa gaaatcggat ttaatgaaaa taccattaaa
      181 aaatatctac aatgcactaa catccagaca gtgacggtgc ctgttcctgc gaagttttta
      241 cgtgcttcaa atgtaccgac tggattgctt aatgaaatga ttgcttatct caactcggaa
      301 gaacgcaatc atcataattt ttcagaactt ttgctttttt cttgcctgtc tatttttgcc
      361 gcatgcaaag gtttcattac actattaact aacggtgtgc tatccgtttc cggaaaagtg
      421 agaaatattg tcaacatgaa gctggcgcac ccatggaagc tgaaagatat ttgtgactgc
      481 ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc aagagcaaac gacattctca
      541 cagattctat tagatgccag aatgcagcac gcaaaaaatt tgatacgcgt agaaggttca
      601 gtcaataaaa ttgccgaaca atgtggttat gccagtacat cttattttat ttatgcgttc
      661 cgcaaacatt tcggcaacag tccgaagaga gtttctaagg agtaccgttg tcaaagtcac
      721 acgggtatga atacgggcaa cacgatgagt gctttagcta tttgattatt tgctaacgag
      781 tagtcaacca cgcacgctac gtaagaatta tatggggcag ccattccctg c
//
LOCUS       DAPHJE010000323          823 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPHJE010000323 DAPHJE010000000
VERSION     DAPHJE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.328"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..86
                     /locus_tag="RH222_004624"
     CDS             <1..86
                     /locus_tag="RH222_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3799511.1"
                     /translation="HGLGMDVIFRRRLHQLIGRNGCCAASS"
     gene            161..>823
                     /locus_tag="RH222_004625"
     CDS             161..>823
                     /locus_tag="RH222_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3799512.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVV"
BASE COUNT          158 a          247 c          269 g          149 t
ORIGIN      
        1 cgcacgggct gggcatggat gtcattttca gaagacgact gcaccagttg attgggcgta
       61 atggctgttg tgcagccagc tcctgacagt tcaatatcag aagtgatctg caccaatctc
      121 gactatgctc aatactcgtg tgcaccaaag cgaggtgagc atggcgacgg acaccccacg
      181 gattccagaa caaggcgtgg ccactctgcc tgatgaggct tgggagcgtg cgcgccgtcg
      241 tgcggagatc atcagtccgt tggcgcagtc ggagacggtc gggcacgaag cggccgatat
      301 ggcggctcag gcgctgggct tgtctcggcg ccaggtatac gttctgatcc ggcgtgcccg
      361 gcaaggcagc ggcctcgtga cggatctggt gcccggccag tccggtggag gtaaaggtaa
      421 ggggcgcttg ccggaaccgg tcgagcgcgt catccacgag ctactgcaaa agcggttcct
      481 gaccaagcag aagcgcagcc tagcggcctt tcaccgcgaa gtcactcagg tgtgcaaggc
      541 tcaaaaactg cgagtgccgg cgcgcaatac cgtggcctta cggatcgcta gccttgaccc
      601 gcgcaaggtc atccgccggc gggaaggcca ggatgccgct cgtgacctac aaggtgtggg
      661 cggcgagcct cctgccgtga ccgcgccgct ggagcaggtg cagatagacc atacggtcat
      721 cgacctgatc gtggtcgatg accgcgaccg gcaacctatt ggccgcccgt acctgaccct
      781 cgccatcgac gtgttcaccc gctgcgtgct cggcatggtc gtc
//
LOCUS       DAPHJE010000324          807 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPHJE010000324 DAPHJE010000000
VERSION     DAPHJE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.329"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..522
                     /locus_tag="RH222_004626"
     CDS             25..522
                     /locus_tag="RH222_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rhsA"
                     /protein_id="HDV3799513.1"
                     /translation="MRGRYITQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPL
                     PIPWPKSPAQQQADDNAAKALTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVA
                     DTGITDRVNDIINDRFWSDGKKPDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQS
                     NDKKR"
     gene            543..>807
                     /locus_tag="RH222_004627"
     CDS             543..>807
                     /locus_tag="RH222_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HDV3799514.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCE"
BASE COUNT          278 a          144 c          180 g          205 t
ORIGIN      
        1 aaccgccacc gctattatga cccgctgcgg gggaggtata tcactcagga tccgattggg
       61 ctgaaggggg gatggaattt ttatcagtat ccgttgaatc cagttacgaa tacagatcct
      121 ctggggttag aagtttttcc tagaccattc cccttgccaa ttccatggcc caaaagccct
      181 gcacagcagc aagcagatga taatgctgca aaagcattga caaaatggtg gaacgataca
      241 gcatcacaaa gaatatttga ctctctaata ttgaataatc cgggactagc attagatata
      301 acaatgatag cttctcgtgg aaatgttgca gacacaggga taactgatcg tgtcaatgac
      361 ataataaatg acagattctg gagtgatggg aaaaaacccg acagatgtga cgtacttcag
      421 gaactaattg attgtggtga tattagtgct aaagatgcaa aaagcacaca gaaagcctgg
      481 aattgtcgtc actccagaca gtcaaacgat aaaaaaagat agcccttgtg gaggttcctg
      541 caatgtcaaa tacataccag aaaagaaagg caagtaaaga gtatggttta tataatcaat
      601 gtaagaaact aaatgatgat gaattatttc gcttacttga tgatcacaat tccttgaaaa
      661 ggatttcatc tgccagagta ttacagttaa gaggtgggca agacgctgtt agattggcaa
      721 ttgagttctg ctctgataaa aattatatcc gtagagatat cggagcattt atactcgggc
      781 aaataaaaat ttgcaaaaaa tgcgaag
//
LOCUS       DAPHJE010000325          802 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPHJE010000325 DAPHJE010000000
VERSION     DAPHJE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.330"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(351..559)
                     /locus_tag="RH222_004628"
                     /pseudo
     CDS             complement(351..559)
                     /locus_tag="RH222_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          240 a          164 c          164 g          234 t
ORIGIN      
        1 tcattcttga ttggttgttg ccctgagaat atagtttcca gggttattac atttcacgaa
       61 gaacgataat atctttaaat cacaaggaca cctaatcaca acaagtttat tgtgcacttg
      121 ttgaagagag tatttgcaaa aacattttat gtcgctaaat aacatgacga tttcattatg
      181 catgttattt ataaaccgtg actaaacaga atattattgc gaacaaggga agtgtcagga
      241 aaggtcaagg gctaccaatg gttaatatgg ctataaatca ttagctatgg ttaatgtttg
      301 cttgctgaaa ctaacaccga attgctctaa acagataagt gcagggttta ttaaagctga
      361 ccgacagact ttcttaacct ggtaatatct ctcttgggtg actctccaaa catccgcgaa
      421 tattcccgac tgaaatggga tagctttcgt aaccgacagc ataggctgct gtggtgacat
      481 cgtaatgttc attcagcatc ggtcgcctga cttcattgag acgtagccat ttctgatacc
      541 ggagtggact cattcccgct tgatccgtcc ttaaataagt aacagcaccc tctggtgttt
      601 tcgtatgcag tgtctatgcg acagacactg cgctcttagc atggggcagg gaataaccag
      661 gctatttctc catgcacagg ccaattatga aagaggagag gcttaagttc ttgaaccctg
      721 aacgaataac gactaccaga aaagcacttc gcatcactca gacatgattt aacttatgtt
      781 gtgcgcaaat ccagttcata at
//
LOCUS       DAPHJE010000326          801 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPHJE010000326 DAPHJE010000000
VERSION     DAPHJE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.331"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..>801
                     /locus_tag="RH222_004629"
     CDS             52..>801
                     /locus_tag="RH222_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3799515.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTDDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNY"
BASE COUNT          263 a          159 c          155 g          224 t
ORIGIN      
        1 ctaagcaaac tatgtgatct ccatttcgat tgatttagtg tttattgacg tatgtactgg
       61 attattaacg ataatatcga gttctggcct gagcaccgaa aattaatatc ggtacataac
      121 gccgatctta acgtcgttct gacaacgcca gccagtcgat gtttatcact tctacttgaa
      181 gcttttcctg atgtggttgc acaacaagat tttttcacca gagtctggga agaagaaggt
      241 atgcgtgtgc ctactaacac gttatatcag aacatatcca ttatcagacg cggatttcgc
      301 gctgttggtg atactaccca ctcgctaatt gcaaccgtgc cgagaagagg attcaagatc
      361 cataatgaca ttaacataca aaatcatgta ataaactcgt caacagacgc acatacacac
      421 aatgccccac ctgccataaa agttaatgcg ggatacaaag agagcattgg tggcgcaaag
      481 aatttcaata acaaaatcct caaacatata aaaagtcatc taattatgtt gagcgcattt
      541 gtcataggcg catattctgc atattggcta tggaataata atcaaccgaa gccatttttc
      601 aaggattaca agactgttgc ggaaattaat ggctgccatt ttaatgtaac agacgataca
      661 attgatgggt tgaaagagtt cgataaatat aaaacacgga tactggattc ggggattaac
      721 tgtaaaaaac atccgtggtt atacttccct cttgcaaaat cctcaccggg gatgattgtt
      781 atggcatgca ataaaaacta t
//
LOCUS       DAPHJE010000327          791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPHJE010000327 DAPHJE010000000
VERSION     DAPHJE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.332"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..651
                     /gene="slp"
                     /locus_tag="RH222_004630"
     CDS             85..651
                     /gene="slp"
                     /locus_tag="RH222_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV3799516.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          238 a          165 c          166 g          222 t
ORIGIN      
        1 atcaggcata tcacctcaga atcagatgaa aactataaag aaatatctat tatggcttta
       61 atatttgttg ataaggatag taacatgaac atgacaaaag gtgcactcat cctcagcctt
      121 tcatttttgc ttgccgcatg tagttcaatt ccgcaaaata tcaaaggcaa taaccaacct
      181 gatattcaaa aaagttttgt tgctgttcat aaccagccgg ggttatatgt tggtcaacaa
      241 gcgcgctttg gtgggaaggt tatcaacgtt atcaatggca aaacggatac gttgttagaa
      301 atcgctgtat taccgttgga tagctatgcg aagcctgata ttgaagccaa ctatcagggc
      361 cgactgctcg ccagacaaag cggcttcctt gatccagtga actatcgtaa tcactttgtt
      421 accatcctcg gtaccattca gggtgaacaa ccaggcttta tcaataaagt cccgtataac
      481 ttcctggaag tgaatatgca gggcatccag gtgtggcatt tgagagaagt ggttaatacc
      541 acctataacc tgtgggatta cggctatggt gcattctggc cggaaccggg ctggggtgcg
      601 ccttactaca ccaatgcggt gagtcaggta acacctgagc tggtcaaata acaccaccga
      661 aagatgcaga tgctttctca gcatctgcat catgtattac ataaaattaa tatacagtaa
      721 gctaactatt attattataa gccctgtcct gttaattacc tttggcaaac tgattataaa
      781 gttaatgtcc g
//
LOCUS       DAPHJE010000328          777 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPHJE010000328 DAPHJE010000000
VERSION     DAPHJE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.333"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(140..682)
                     /locus_tag="RH222_004631"
     CDS             complement(140..682)
                     /locus_tag="RH222_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799517.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          245 a          159 c          137 g          236 t
ORIGIN      
        1 aattccggca tgtgcaggaa ggaccataga aaaaatgagt aaagataata atcctttcat
       61 tttaatgcct cttaaattaa aaggccgcca tgggatagcg gccatgttta ttacctggag
      121 aaacttacaa ttttatttat taatcctgcg ccacacgcat aaccagagaa gataacacat
      181 cacctgattg cactgagtta tctgtcagtt tcaggtattt tgctttaaac ggaatagcat
      241 attgaccttc cgctgtttta taactctcaa tgttaaaagg tgcagaaaca tccaggacat
      301 cgctgttatc acctaaatag agatagtaac ccacggtatc gttgctactg gctatcgcct
      361 tatcgttacc ggttatttta tcgttggtaa tcaacgagag ttttacacca gcaacttctt
      421 tcgggcagct aatttttaac atgctgctgg ttacaccgac tgcatcatca taggtagaat
      481 atttagcttt accttcaaag acagtggttg gtacagtcgg catattaata cttactgaat
      541 caccacccgc ttcgaattta cacggagatt ctgcaatatt ccccttaata gttaatgttc
      601 cggtatcaac gtctgcagca aatactccag cgctcataaa aaaagaagaa aagacagcgg
      661 cagcaataat tgaacgtttc ataatatgtt ccttaataaa atttattttt ggttgttaag
      721 agtctgtatc tcttggaaac aaatttctca catcaaaaaa gttgccgcaa tgtaaac
//
LOCUS       DAPHJE010000329          775 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPHJE010000329 DAPHJE010000000
VERSION     DAPHJE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.334"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>775)
                     /locus_tag="RH222_004632"
     CDS             complement(<1..>775)
                     /locus_tag="RH222_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3799518.1"
                     /translation="SDNNGFSLPQDVRLLELIKNPFYLSEYLRFYTGESIDYVSFKEK
                     LWNKIIVKNKPSREQCFLATAFQRASEGQFFVSPACDTGILDELVKDGIVGYEAAGYF
                     ITHDIYEEWALEKKISVDYIRKANNNEFFEKIGESLPVRRSFRNWISERLLLDDQSIK
                     PFIAEIVCGEGISNFWKDELWVAVLLSDNSSIFFNYFKRYLLSSDQNLLKRLTFLLRL
                     ACKDVDYDLLKQLGVSNSDLLSIKYVLTKPKGTGWQSVIQ"
BASE COUNT          254 a          160 c          122 g          239 t
ORIGIN      
        1 ttggatcaca ctctgccaac cagttccctt aggcttagta agaacatatt taatggaaag
       61 cagatctgaa ttacttacac ctaactgttt aagcagatcg taatcaacgt ccttgcaagc
      121 aagcctcaat aagaaagtaa gtctttttaa tagattctgg tcactactaa gtaaatatct
      181 tttaaagtaa ttaaaaaata tgcttgaatt atcggaaaga aggacagcta cccataactc
      241 gtctttccaa aaatttgata ttccttctcc acagactatt tctgcgataa aaggctttat
      301 ggactggtca tctaaaagca atcgttcaga tatccaattc cgaaaactac ggcgaacagg
      361 aagtgattct cctatttttt cgaagaactc gttattgttc gctttacgga tataatcgac
      421 agaaattttc ttttctaatg cccattcctc gtatatatca tgtgtaatga agtaaccagc
      481 agcttcatag ccgacaattc cgtctttaac taactcatct aaaattccag tatcacatgc
      541 cggggagaca aaaaattggc cctcactagc ccgctgaaaa gcagtcgcta agaaacactg
      601 ctcccgagaa ggtttatttt tgacaataat cttattccaa agcttttctt tgaagctcac
      661 ataatcgatg ctttcaccgg tatagaacct caaatattca cttagataaa atggattttt
      721 gattagttct aataatcgaa catcttgagg aaggctaaat ccattgttat cagat
//
LOCUS       DAPHJE010000330          769 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPHJE010000330 DAPHJE010000000
VERSION     DAPHJE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.335"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..760
                     /locus_tag="RH222_004633"
     CDS             44..760
                     /locus_tag="RH222_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3799519.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          256 a          130 c          167 g          216 t
ORIGIN      
        1 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
       61 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      121 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      181 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      241 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      301 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      361 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      421 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      481 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
      541 taatattgtt atttatactg aacctactga ccttcataaa ggaatcgcgg cgctaaaagc
      601 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
      661 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
      721 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa tgatatttt
//
LOCUS       DAPHJE010000331          758 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPHJE010000331 DAPHJE010000000
VERSION     DAPHJE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.336"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..250)
                     /locus_tag="RH222_004634"
     CDS             complement(<1..250)
                     /locus_tag="RH222_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799520.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYK"
     gene            444..>758
                     /locus_tag="RH222_004635"
     CDS             444..>758
                     /locus_tag="RH222_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799521.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKL"
BASE COUNT          248 a          116 c          127 g          267 t
ORIGIN      
        1 atttatatat gctcatagca gataatattg cttgctgaat tatctcttca gaaatatccg
       61 ctatctttat tgacaaatca tgtgttagat attttcctcc aagagattct tgttcaatat
      121 aatttaaacc agaccccatt gtgattttaa ctggattaac ggatttctct tcatttttct
      181 tatttttgtt ctcctgatca ggaactaatg gttgcagtgg taggctatta ttaatatttc
      241 gtggcttcat tttacatttc tctcatttga ttagagcaaa atccgaattc gcaacttctg
      301 ataatgtcaa atatcacctt atggagacgt tacgtagaat gactatattt gttatcggca
      361 cattaggggt attaatttta gtattgatgg aaggtgatta ttgaacagta acatcgagtc
      421 acggtaaaaa tgtgaggtat acaatggaca ttgaattttc gcagattcat gaaatggtct
      481 atatgcatga tattgtaaat tcggactcga aaaagaaacc aaggattccg ttaaaaaaat
      541 tccttaatgc tgagaatgtt ctcacgcaaa caacatcatg gacattaaaa tcaagatatg
      601 tgaatgttaa ttctgtcaat aaagtcaacg taaagagtaa agttaaaaac agttatatct
      661 cacgatctgt caatgatgag tttagtttaa cagatgatga aatcaattct tttaaagaaa
      721 cgttagtcct ttcctctatt gattcactga gtaaactg
//
LOCUS       DAPHJE010000332          751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPHJE010000332 DAPHJE010000000
VERSION     DAPHJE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.337"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..>751
                     /locus_tag="RH222_004636"
     CDS             16..>751
                     /locus_tag="RH222_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3799522.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLG"
BASE COUNT          235 a          167 c          140 g          209 t
ORIGIN      
        1 taaccggagt agtgtatggc caatctctac gacttgaaaa agttcgactt aaaccttctt
       61 gtcatatttg agtgtattta ccagcattta agcattagca aagctgctga atcactatat
      121 atcaccccat cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct
      181 ttattcattc gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac
      241 catcttgaga aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa
      301 ctaaagaaaa actttattat ctacggccct cagctcattt cttgctctaa taatagtatg
      361 ctgatccgct gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg
      421 tcagcagaga atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa
      481 atgccagtga tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta
      541 atttgtagta ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca
      601 gaagagttca cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata
      661 gacgagagat ttatgaatcg caaaatctca tttcgtggtt cctctttgct gacaatcatt
      721 aatagcattg cagttacaga tttactggga a
//
LOCUS       DAPHJE010000333          741 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPHJE010000333 DAPHJE010000000
VERSION     DAPHJE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.338"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..>741)
                     /locus_tag="RH222_004637"
     CDS             complement(45..>741)
                     /locus_tag="RH222_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HDV3799523.1"
                     /translation="DENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTP
                     TSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNV
                     IGPDTLWTWWGRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPIS
                     NMQGTPEKPSDIR"
BASE COUNT          203 a          169 c          141 g          228 t
ORIGIN      
        1 aaaataaaaa aatagtaaca agcatgcttt catacatcta caacctatct tatatcactg
       61 ggtttttctg gcgtaccttg catattagaa atggggtgcc cagatggccc atatgttatc
      121 caattaccaa ggtcttttgt tgttatagcc attagatcct tatttgaatt aaggttcgtt
      181 ggtgctgtta gcactgtatc catttttaac ttaccattag ttcctcgccc ccaccacgtc
      241 catagcgtgt caggtcctat tacatttgta tgtaactctt tagctaattg gctagcaaat
      301 gaattttgtc ctttacctgt attacaagaa aataaccaaa ctgtcatccc atctttataa
      361 ttcccatcaa atttaattag atatgctaga tctttagctg tcatgatact gcgcgttgcg
      421 gattcaatag atgtgggggt accatgcccc ccgattgtga aaacgcctgg gatattaatc
      481 tcatcagcta cagaatggat aagatcactt tcggggtata aatttatatc aaccaaacct
      541 tgcggatcta catttatgac cggattcaac ggatactgat aaaaattcca tccccccttc
      601 agtccaatcg gatcctgagt gatataccgc ccctgcagcg ggtcataata gcggtggcgg
      661 ttgtaataca ggccggactc ctcatcatac tgctgacccg gcaggcgaat gagctgctgc
      721 agatgatgcg ggttctcatc a
//
LOCUS       DAPHJE010000334          737 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPHJE010000334 DAPHJE010000000
VERSION     DAPHJE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.339"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..678
                     /gene="fimE"
                     /locus_tag="RH222_004638"
     CDS             82..678
                     /gene="fimE"
                     /locus_tag="RH222_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV3799524.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          207 a          139 c          180 g          211 t
ORIGIN      
        1 tatctattgg gctaattttg accgattgag gtttcctata ggtattcatt caaatatatc
       61 tcagttagga gtactactat tgtgagtaaa cgtcgttatc ttaccggtaa agaagttcag
      121 gccatgatgc aggcggtttg ttacggggca acgggagcca gagattattg tcttattctg
      181 ttggcatatc ggcatgggat gcgtattagt gaactgcttg atctgcatta tcaggacctt
      241 gaccttaatg aaggtagaat aaatattcgc cgactgaaga acggattttc taccgttcac
      301 ccgttacgtt ttgatgagcg tgaagccgtg gaacgctgga cccaggaacg tgctaactgg
      361 aaaggcgctg accggactga cgctatattt atttctcgcc gcgggagtcg gctttctcgc
      421 cagcaggcct atcgcattat tcgcgatgcc ggtattgaag ctggaaccgt aacgcagact
      481 catcctcata tgttaaggca tgcttgcggt tatgaattgg cggagcgtgg tgcagatact
      541 cgtttaattc aggattatct cgggcatcga aatattcgcc atactgtgcg ttataccgcc
      601 agtaatgctg ctcgttttgc cggattatgg gaaagaaata atctcataaa cgaaaaatta
      661 aaaagagaag aggtttgatt taacttattg ataataaagt taaaaaaaca aataaataca
      721 agacaattgg ggccaaa
//
LOCUS       DAPHJE010000335          728 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPHJE010000335 DAPHJE010000000
VERSION     DAPHJE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.340"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            319..541
                     /locus_tag="RH222_004639"
                     /pseudo
     CDS             319..541
                     /locus_tag="RH222_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          213 a          155 c          160 g          200 t
ORIGIN      
        1 gagaatttca tgaactaaca tggattgcgt ctcttatgag catttcttat agcatgcaat
       61 acttcaactt ttcccatcgt agtcgaaacc ctgccacatc aatctttagg gggtatctga
      121 ttgttttctt gaacaatcca tcagttttgg taacatttag atgcgctaat acttaatcgg
      181 aaatggtgtc tctgagggcg gtagcgttgt cagggcaggc tgggtttacc tagggctatc
      241 ggtagcgaaa attgatctta cccagcaata gtggacacgc ggctaagtga gtaaactctt
      301 ccgtcagagg tgactcacat gacaaaatca gaatcaacca gcaaaaaaaa acgtaaacag
      361 cattcgcctg aatttcgcag tgaagcttag aagcttgcgg aacgcatcgg tgttgctggc
      421 gcagtccgtg aactcaacct gtatgaatca aagcactata actggcgcag taaacagcaa
      481 catcagcaga cgttcctctg aacgtgagct ggagatgtcc accgagatag cacgtctcaa
      541 acaccagcag gcatgtacat aaagccctga ttgcaagcgt actcttgttt ctttcgacat
      601 tagttgaaat gattaactat gtactcaatt tactatggtg cgttaggctg agaaggtctg
      661 atttgaacca aagcgagact ttactttttt acgaaagcct caaatagtca ttgcaaatga
      721 tatcgttt
//
LOCUS       DAPHJE010000336          711 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPHJE010000336 DAPHJE010000000
VERSION     DAPHJE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.341"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            75..281
                     /locus_tag="RH222_004640"
     CDS             75..281
                     /locus_tag="RH222_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3799525.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            368..>711
                     /locus_tag="RH222_004641"
     CDS             368..>711
                     /locus_tag="RH222_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799526.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRS"
BASE COUNT          166 a          195 c          181 g          169 t
ORIGIN      
        1 ttatgccggt gaaggtggtg cgttgttttc atgaccacac cggctggttt aacaacatcc
       61 ggaggaacat tctcatgatc accccagttt cactgatgga tgaccagatg gtcgacatgg
      121 catttatcac tcaactgacc ggcctgaccg ataagtggtt ttacaaactc atcaaggatg
      181 gtgactttcc tgcgcccatc aaaatgggcc gcagctcccg atggctgaaa agcgaagtgg
      241 aagcctggct gcaggcgcgt attgcacagt cccgtccgta attcttaacc gttcatctgc
      301 agcagacgca gtctccggcc tgccggcggc gttctgctgc ctgttatccc ccgtgaggaa
      361 tataaaaatg aaaccacagt atcagacccg ttacgagctg ctccacgaaa gctaccagaa
      421 atggctgacc ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcta
      481 ctacttccat aatctgacgc ccgggtgggt gtcattcaat ggtgaaaaac cggagattgc
      541 catcgtaccc cagagtctgc accggctgat ttatggtccg gacaaacgga ccacgccgcc
      601 cctggatgat gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc
      661 gatgctggaa ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt c
//
LOCUS       DAPHJE010000337          710 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPHJE010000337 DAPHJE010000000
VERSION     DAPHJE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.342"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>710)
                     /locus_tag="RH222_004642"
     CDS             complement(<1..>710)
                     /locus_tag="RH222_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3799527.1"
                     /translation="YTNQEWGQGRKSHEPEGDKNADNYLETVGNSNDPKIKIEVDQKA
                     YESGIEIVWRVASFFESPFVIVENEKIAKHFFSLNESIFDLLEEKRKHTENVLYEIQT
                     NIEFKDRSIEIDRRHCIELLHENLVQKKIVIVSGEGGVGKTAVIKKIYEAEKQYTPFY
                     VFKASEFKKDSINELFGAHGLDDFSNAHQDELRKVIVVDSAEKLLELTNIDPFKEFLT
                     VLIKDKWQVVFTTRNNYLA"
BASE COUNT          198 a          146 c          107 g          259 t
ORIGIN      
        1 gccaagtagt tgttacgggt tgtgaaaaca acctgccatt tatcctttat taaaacagtc
       61 aggaattctt taaaaggatc gatattggtc agttctaaaa gcttttcagc agaatctacg
      121 actatgactt tacgtaattc gtcttgatga gcattagaga aatcgtctaa gccatgcgca
      181 ccgaatagct cattaatact gtccttttta aactcgctag ccttaaagac atagaaagga
      241 gtgtattgtt tttctgcttc ataaattttt ttgataactg ctgtttttcc aaccccacct
      301 tctccgctga cgatgacaat ttttttctga actagattct catgtagaag ttctatacaa
      361 tgtcgtctgt caatttcaat acttctgtct ttgaactcta tattggtttg aatttcatat
      421 aaaacatttt ctgtgtgctt gcgcttttct tctaataaat caaagatgct ttcattaagg
      481 gagaagaaat gtttagcaat cttttcattc tcaactatta caaacggtga ttcaaaaaaa
      541 ctagcaactc tccatactat ttcgataccc gactcatatg ctttctgatc aacttcaatt
      601 tttattttgg gatcgttact atttccgaca gtttccaaat aattatcagc gttcttatcg
      661 ccttcaggtt catgggactt tctcccctgc ccccactctt gattagtata
//
LOCUS       DAPHJE010000338          707 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPHJE010000338 DAPHJE010000000
VERSION     DAPHJE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.344"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..645
                     /gene="fimA"
                     /locus_tag="RH222_004643"
     CDS             82..645
                     /gene="fimA"
                     /locus_tag="RH222_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3799528.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          180 a          138 c          171 g          218 t
ORIGIN      
        1 ttcttgtttt taagcaataa cagaatgact taattgttaa ctctctttat tcagctactt
       61 aaatataaat tttggagaat tatgaaactc aaacatgttg gtatgattgt cgtttctgtg
      121 ttggcgatgt cgtctgctgc ggtaagcgca gccgagggtg atgaatcagt aacgaccact
      181 gttaatggcg gtgttattca ttttaaaggt gaagtggtaa atgccgcttg tgcaattgat
      241 tcagaatcaa tgaaccaaac ggttgagctg ggtcaggttc gttcttctcg cctggctaaa
      301 gcgggtgacc tcagctccgc cgttggcttc aatatcaagc tgaatgattg tgataccaat
      361 gtttccagta atgcagctgt tgcattcctg ggtactactg tcaccagtaa tgacgatacg
      421 ttagcgctgc agagttcagc ggcaggctct gcccaaaatg tcggtattca aattttggac
      481 cgtacgggtg aggtattaat acttgatggg gccactttta gtgctaaaac cgacttgatt
      541 gatggcacga atatactgcc attccaggct cgttatattg ctctcgggca gtccgtagct
      601 ggtactgcaa acgctgatgc gaccttcaaa gttcaatatc tataattcaa tcttaaggtg
      661 gctatacgtc atctgaaatt gaaacagggt gtgtgtggca tggctac
//
LOCUS       DAPHJE010000339          704 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPHJE010000339 DAPHJE010000000
VERSION     DAPHJE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.345"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..76
                     /locus_tag="RH222_004644"
     CDS             <1..76
                     /locus_tag="RH222_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV3799529.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(96..161)
                     /locus_tag="RH222_004645"
                     /pseudo
     CDS             complement(96..161)
                     /locus_tag="RH222_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            394..498
                     /locus_tag="RH222_004646"
                     /pseudo
     CDS             394..498
                     /locus_tag="RH222_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          233 a          142 c          139 g          190 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac ctcctcccga actcaaagct atcatacaga
      121 taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac ccagcaatag
      181 tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga caaaacagtt
      241 tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag atctgaagct
      301 tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg aatcaccaca
      361 gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta tatcgagtgc
      421 gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga acaatttgaa
      481 aatcagaatc tcacttaaaa cggcatccat tttacaaaag atggccgtct gtgtatttat
      541 aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata tcctaaaaat
      601 tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga gttgactgaa
      661 tataatacag cctcttccta cataagaagg aatgtgctca caaa
//
LOCUS       DAPHJE010000340          701 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPHJE010000340 DAPHJE010000000
VERSION     DAPHJE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.346"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..626)
                     /locus_tag="RH222_004647"
     CDS             complement(<1..626)
                     /locus_tag="RH222_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV3799530.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQI"
BASE COUNT          220 a          136 c          156 g          189 t
ORIGIN      
        1 aatatttgtg gccagggatt gttttccgcc aaatatcgcg ttgcagaaag tcggcaaata
       61 attaacactt tgtctccttt agtgaaaaat tttccttctt caggctccag tagtaaataa
      121 tgtatttggc caaattgatc ggtgagtttt ccttcgcagg gattacctga agtggcttga
      181 tgaccggtaa ttaatgccat gctaccaata tattcttctt ctgtaatagc tgaactatgg
      241 tcgcgtggaa tccagggcgc aactatttta ccggtataat gcaccgcaat aatcgtaaac
      301 agtaaactga caggaaccac gaacaggttc gacagtggtg attgccagac catgatgcag
      361 gcatgctgca acaggatgcc aataagacca aaaaaaccag ccagcaagca gagaacgacg
      421 agagctggca atcttccgat attgagataa tgaagtgcct ggctaatatg acctgtggtt
      481 atggaatcat aatggtcaag atgtgcatca agagcaccgg agagcatgtg accgcagata
      541 agcgctaaaa tttctaaaag gccgatcaac agtacaaagg aaatagcgaa aagataaggg
      601 gtattatagt cggcgaataa aatcatatgt cactccaatg tccatttagt gacgcaatat
      661 ttactttggg atagtttcct tgcttacatt tatctttgtt a
//
LOCUS       DAPHJE010000341          696 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPHJE010000341 DAPHJE010000000
VERSION     DAPHJE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.347"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            94..636
                     /gene="ecpR"
                     /locus_tag="RH222_004648"
                     /gene_synonym="matA"
     CDS             94..636
                     /gene="ecpR"
                     /locus_tag="RH222_004648"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV3799531.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
BASE COUNT          239 a          113 c          157 g          187 t
ORIGIN      
        1 taaggaaact gaatgcacct gtaaaaatta caggtttgga aagtagtgac atggcaaagt
       61 gattacagta gggactatga ggttaaaaac catatggaat gtcaaaaccg ttctgataaa
      121 tacatctggt ctccccatga cgcctacttc tataaaggac tatctgaact gattgtggat
      181 atcgacagat taatttatct atcgttggag aaaattagaa aagatttcgt gtttatcaat
      241 ctcagtacgg attctttatc tgaatttata aaccgtgata atgaatggtt atccgcggta
      301 aaggggaaac aggtcgtatt gattgcggcc agaaagtcag aagccttagc aaattattgg
      361 tattacaata gcaatattag gggcgtggta tacgctggac tgagtcgtga tattagaaaa
      421 gaactggcct atgtgattaa tggcaggttc ctgagaaaag atattaagaa agataaaatc
      481 acggaccggg aaatggaaat tatccgcatg acggcccagg gaatgcaacc taaatcgatt
      541 gccagaattg aaaattgtag tgtgaagaca gtgtataccc atcggcgtaa tgctgaggcc
      601 aagctgtact caaaaatata taagttggtt cagtaaactc caggcaagtt agttttaaaa
      661 aatgactcac tgggacatca cgtcctcaat tcaact
//
LOCUS       DAPHJE010000342          695 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPHJE010000342 DAPHJE010000000
VERSION     DAPHJE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.348"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..258
                     /locus_tag="RH222_004649"
     CDS             <1..258
                     /locus_tag="RH222_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDV3799532.1"
                     /translation="ENELTYQFQRWGQNHTVRILESSEGIRLKPSDTLVSNRLREAQH
                     NDVTAQRWVLTEQDERQGQRHQPYEEQENESKFENDQKDES"
     gene            258..>695
                     /locus_tag="RH222_004650"
     CDS             258..>695
                     /locus_tag="RH222_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDV3799533.1"
                     /translation="MFGLRKVNRNTHMFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     RVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDK"
BASE COUNT          241 a          121 c          164 g          169 t
ORIGIN      
        1 gaaaatgaat tgacatatca gtttcagcgt tggggacaaa atcatacagt ccggattctg
       61 gaaagtagtg aagggatacg actaaaacca tcagacacac ttgtctccaa cagactccgt
      121 gaagcccaac acaatgatgt tacagcacaa cgttgggtat tgactgagca agatgaacga
      181 caaggacaac gacatcagcc gtatgaggag caagaaaatg aaagcaaatt cgaaaacgat
      241 cagaaggatg aaagttaatg ttcggtctga ggaaagtaaa tcgaaacact catatgtttg
      301 aaaccacttt tcaaaattgg aaagaaaatg gcgaagatgt tgcattatta atgcccgaat
      361 ttagtgctaa atggctaccg atagcagaag agagtggcag ctggtctgga tgggtattgc
      421 ttggagaaat atttccctta atttcatctg agctcgctgg gatggcttta atgccagaaa
      481 cggaaaggtt aataggggaa tggctcagtt tatcaagttc cccgttaaat ctaaagcatc
      541 ctgaactaaa atataatcgt ttgcgcgtag gtaaagtatt tgatggagta ttgagccctg
      601 ctcaaccact aataagaata tggacagggg aattgaatct ttggttagat aaagtcacag
      661 tctgtcaata cggaaacgct ccagcgttag acaaa
//
LOCUS       DAPHJE010000343          691 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPHJE010000343 DAPHJE010000000
VERSION     DAPHJE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.349"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(31..>691)
                     /locus_tag="RH222_004651"
     CDS             complement(31..>691)
                     /locus_tag="RH222_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV3799534.1"
                     /translation="LFLGSSCIYPKLANQPISEAELLQGTLEPTNEPYAIAKIAGIKL
                     CESYNRQYNRDYRSVMPTNLYGPFDNFHPTNSHVVPALIRRFHEAKENDQAEVIVWGS
                     GNPKREFLYVDEMAAASVYVMELNKNVYDQFTEPMLSHINVGTGRDCSIKELASTIAS
                     VVGYKGKITFDSTKPDGTPRKLLDVSRLAALGWTSKLSLHDGLVDTYQWYLDNQDTFR
                     R"
BASE COUNT          204 a          147 c          122 g          218 t
ORIGIN      
        1 attaataaat tcttcatccg ttaacattta tcaccttcta aaggtatctt gattatctaa
       61 ataccattga taggtatcaa ccaacccatc gtgtaaagat aattttgaag tccatcccag
      121 tgcggccaaa cggctcacat ctagcaattt cctaggtgtg ccatccggtt tagtactatc
      181 gaaagtaatc ttgcctttat aaccgaccac actagcaatt gtagatgcta attcttttat
      241 cgagcaatcg cggccggtgc caacattgat atgagagagc attggttccg taaactgatc
      301 gtatacattt ttattcaact ccatgacata tacgctggca gcagccattt cgtcgacata
      361 taaaaattca cgcttaggat ttccgcttcc ccagactata acctcagcct gatcattttc
      421 ctttgcttca tggaaacgtc tgattagcgc aggtaccaca tgagaattag ttggatggaa
      481 gttatcaaaa ggcccatata gattagtagg cataactgac cggtaatcgc gattatattg
      541 tctgttatat gattcgcata attttatacc tgcaattttt gctattgcat aaggttcatt
      601 cgtcggttca agcgttcctt gtaaaagttc agcttcactt atcggctgat tagcaagttt
      661 aggatatata caggaagaac ctaaaaacaa t
//
LOCUS       DAPHJE010000344          678 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPHJE010000344 DAPHJE010000000
VERSION     DAPHJE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.350"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            200..661
                     /locus_tag="RH222_004652"
     CDS             200..661
                     /locus_tag="RH222_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799535.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          253 a          115 c          124 g          186 t
ORIGIN      
        1 atctgtaaga ttagctaagc aggaccaagg atataagaat cattatttat cagatgaaat
       61 gttagccgga aagaaagaac tttacgactt cacccctgaa agcatttaca gggccatgac
      121 tatatttgat agattacaaa ataaaagtga tatacagacg ctaaaaacag aatgttattg
      181 ccttctagcg gaatgccata tgtctttggc acttcatgga aaaagtgaac ttgaacttgc
      241 tgctcaaaaa gcattagagc ttttagatta tgtatcagac ataaccactg tcgatggaaa
      301 aattttagct attatgggac tgataactgg tctgtctgga caagcaaaag tatctcatat
      361 cttatttgaa caggctaaga tacactcaac tgatatagcc tctctctact actatagggc
      421 actagtccac tttcataatg aaaaaattga agaggcaagg atttgtatag acaaatcact
      481 acaactcgaa cccagaagac gaaaagcagt tgtgataaaa gaatgtgtag atatgtatgt
      541 gcctaacccg ctcaaaaaca acattaaact ctactataaa gaaactgaga gtgaaagcca
      601 tcgagttata attgacaaca ttttgaaatt aaagcagctg acgagaattt gtatgcgata
      661 aatttttatt gacgcaat
//
LOCUS       DAPHJE010000345          673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPHJE010000345 DAPHJE010000000
VERSION     DAPHJE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.351"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   5..521
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5..32
                     /rpt_unit_seq="gagttccccgcgccagcggggataaacc"
BASE COUNT          169 a          182 c          187 g          135 t
ORIGIN      
        1 tacagagttc cccgcgccag cggggataaa ccgtccacgc tgtaacggcc atcattaagt
       61 ttagtgagtt ccccgcgcca gcggggataa accggctgat ggtctgggag tgtccatcgg
      121 gcaactgagt tccccgcgcc agcggggata aaccggaagt aggcctgaca gtgattgaac
      181 gcatactgag ttccccgcgc cagcggggat aaaccgagtt ggggcggcgc aataacgaga
      241 cgatacgcga gttccccgcg ccagcgggga taaaccgggg agtggcactt ctggggtagc
      301 ggcggccctg agttccccgc gccagcgggg ataaaccgtc aacgcgctca gacgttgcgt
      361 gagtgaacca gagttccccg cgccagcggg gataaaccga aatatccagg gctgggctgg
      421 aggcagacgg cgagttcccc gcgccagcgg ggataaaccg cccggaatgc attctgaagg
      481 tttgctgtat atgagttccc cgcgccagcg gggataaacc accatataac ccgttatctc
      541 tttctcaagt ttttatatta gctgtacttg taataagcaa catatccacg taacacctca
      601 tgttcaaaat agttccccat gccagataag ttcacaatta tcgatacaaa aaatcaaatt
      661 taatcaaagt ttt
//
LOCUS       DAPHJE010000346          670 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPHJE010000346 DAPHJE010000000
VERSION     DAPHJE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.352"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>670
                     /gene="espL4"
                     /locus_tag="RH222_004653"
     CDS             <1..>670
                     /gene="espL4"
                     /locus_tag="RH222_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDV3799536.1"
                     /translation="AAQYINDIYQNVNYKPHQDDYSSAEKFLTHFNKKCKNQTLALIS
                     SRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGCT
                     HYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIP
                     ALPKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLT
                     AYNSG"
BASE COUNT          226 a          126 c          130 g          188 t
ORIGIN      
        1 gctgcacaat atattaatga tatttatcag aatgttaatt acaaacccca tcaagatgat
       61 tactcttcag ctgaaaaatt tctcacgcac ttcaacaaaa aatgcaaaaa ccagactttg
      121 gcgttgattt ccagccgtcc tgaggggcgt tgtgttgctg cctgcggtga tttcgggcta
      181 gttatgaagg catattttga caagatggaa tcaaatggca tcagtgttat ggctgccata
      241 ttattggtag ataaccatgc tttgacggcc cgactaagaa taaagaacac aactgaagga
      301 tgtacccatt acgtggtttc ggtttatgat cctaatgtaa ctaacgataa aataagaatt
      361 atgagcgaaa gcaaagagga tattaaacac tattctctga tggattttat gaatgtagat
      421 tatagcctcc tgaaatggtc aaatgatcat gttattaacc aatctgttgc aataattcca
      481 gcacttccga aagaacagct attgatgtta aaaggatctg tggatgaaat aacccctcca
      541 ttatcaccag caacgatgaa tttgctaatg gcaattggtc agaatcacca acttacgcaa
      601 ctgatgattc agctccagaa aatgccagaa ctacatagaa cagaaatgtt gactgcctat
      661 aatagtggac
//
LOCUS       DAPHJE010000347          667 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPHJE010000347 DAPHJE010000000
VERSION     DAPHJE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.353"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..619
                     /locus_tag="RH222_004654"
                     /pseudo
     CDS             1..619
                     /locus_tag="RH222_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          225 a           97 c          142 g          203 t
ORIGIN      
        1 attgtagttt cagatcagca gccgtttatg attgatggga taattggatt tctcggacat
       61 tatcctgatt tatatgaggt tgttgggggg ctataaagat ctgaagaaat ctatagcgga
      121 gtgtaacaaa tctacagcct aaatatttat tttaggagaa tttgcaggtg gaatgatggg
      181 gtccgaactc gtaaaatggg ttaaatcgca taagatagat gctcatatta ttacatttgt
      241 agcaaagatg ccgtatattg attcaataaa attgcttgaa gcaggtgcga aaggatgcgt
      301 atggaaaacc agtcacccgg cgaaactaaa tcgtgctatt gattcgatta gtaatggtta
      361 cacttatttt gatagtgtac atatggattg tgaaaaaata tcttccaggt attcctctga
      421 taatcaactt acaaatcgtg agtcagaaat attacaactc atagctgatg gtaaaacaaa
      481 caaagaaatc gctaatttct tgcagttaag cagaaaaaca gtcgaaactc atagactcaa
      541 tatcatgaag aaattagatg ttcacagtgg tatcgagttg atcaaaacag ctttacgtat
      601 gggtgtttgc actatataaa aaaaagaggc tttaggctca ttttgctgaa gccttacgat
      661 tgtctag
//
LOCUS       DAPHJE010000348          656 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPHJE010000348 DAPHJE010000000
VERSION     DAPHJE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.354"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..384
                     /locus_tag="RH222_004655"
     CDS             148..384
                     /locus_tag="RH222_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV3799537.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            427..>656
                     /locus_tag="RH222_004656"
     CDS             427..>656
                     /locus_tag="RH222_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV3799538.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRIKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATAD"
BASE COUNT          216 a          113 c          132 g          195 t
ORIGIN      
        1 tgcaataatt tttatcataa cttgttgttt tatatgtgtt agcactaaat gcaaaaaaat
       61 aatttatcat tctgtacaca tatttcgtac aagtttgcta ttgttacctc acttaacatt
      121 gattaacatt tttaacagag gcgtagcatg catcaaaatt cagtgacttt agattctgcg
      181 ggagcaatca ctcgttactt tgcaaaagct aacttgccta ctcagcagga aactctcggg
      241 gaaattgtga ctgagatttt gaaagatgga cgtaatctga gtcgaaagtc gctttgtgcc
      301 aaacttctat gccgactgga acaagcgaca ggggaagagg aacagaaaca ttataatgca
      361 ctaattgggt tgctttttga ataatgcaac agcctgatta tttaacaggt tagctatgaa
      421 gtcgttatga agacatctga taatgaacgt ataaaatatg aaattactgg ccaggcggtg
      481 ctccagatac tgcgcataaa gataaatttt tcattgcaga ctcttattaa gcaattactc
      541 gtaatgaaat cggctgagga agatgctttc cgacgcgatt taattgacag cataattcgt
      601 gattttagca atagtgattc aggagggcca aaccgaagaa cagcaacagc tgacaa
//
LOCUS       DAPHJE010000349          625 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPHJE010000349 DAPHJE010000000
VERSION     DAPHJE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.355"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..537)
                     /locus_tag="RH222_004657"
     CDS             complement(<1..537)
                     /locus_tag="RH222_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799539.1"
                     /translation="MSYQYSQEAKERISKLGQSEIVNFINEISPTLRRKAFGCLPKVP
                     GFRTGHPTEIKEKQKRLIGYMFQSHPSSEERKAWKSFSLFWQFWAEEKIDKSFSMINN
                     LGLKENSGSIFIRELAKNFPKVARENIERLFIFSGFANDPDVINAFNLFPPAVVLARD
                     IVVDTLPIRLDELEARISL"
BASE COUNT          199 a          126 c          104 g          196 t
ORIGIN      
        1 taaactaata cgtgcttcaa gctcatctaa acgaattgga agagtatcaa ccacgatatc
       61 gcgggcaaga acaactgcag gaggaaaaag gttaaatgca tttataacgt ctggatcatt
      121 agcaaaccca ctaaagataa acaggcgctc gatattctct ctagcaactt taggaaagtt
      181 tttagcaagc tctctaataa aaatagagcc agagttttct tttaatccta aattattaat
      241 catactaaat gatttgtcaa ttttctcttc agcccaaaac tgccaaaaaa gagaaaaact
      301 tttccatgct tttctctcct cagaggaagg atgtgactgg aacatatacc caatcaatct
      361 tttctgtttt tctttaattt cagtgggatg tcctgtcctg aatcccggta cttttggtaa
      421 acaaccaaaa gctttacgtc gtaaagttgg agaaatctca ttgataaagt taacaatttc
      481 ggattgtccc aacttagaga tccgttcctt tgcctcttgg ctatattggt aactcattcg
      541 aaggcacctt atctaaagcg gttacattca aatccattgg tcttaacttc ttttaccaat
      601 gctctataat gcggtaataa gtgtg
//
LOCUS       DAPHJE010000350          601 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPHJE010000350 DAPHJE010000000
VERSION     DAPHJE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.356"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..336
                     /locus_tag="RH222_004658"
     CDS             <1..336
                     /locus_tag="RH222_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDV3799540.1"
                     /translation="IEEKHDAMQSLVLSPPARQALAQAALTYRYGDEHQPVTTADILT
                     PRRREDYGQDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVM
                     AETLLESLR"
BASE COUNT          143 a          172 c          166 g          120 t
ORIGIN      
        1 atagaggaga agcatgatgc catgcagtcg ctggtcctgt cgccaccggc acgccaggcg
       61 ctggcacagg cggcactgac ttaccgttat ggtgacgaac atcagccagt caccaccgct
      121 gacattctga caccacgacg cagggaggat tacggtcagg acctgtggag cgcatatcag
      181 accatccagg agaatatgct gaaaggcgga atttccggcc gcagtgcaaa aggaaaacgt
      241 atccacaccc gtgccattca caacatcgac accgatatta agctcaaccg cgcattgtgg
      301 gtaatggcag aaacgctgct ggagagcctg cgctgatgca gcatgtccct gaaagcgtga
      361 ttcaggcacc agaatcaccc gacgccccgg aggcgctgcc ggggtgtcgt tccatttctg
      421 atgatgacct gtgcgcctgg tgtacccgtc tgctttaccg tcccggagaa atcagtctgt
      481 gcagactgag tatacaggca atggcatctg gccatccgtc tgtgaccgga acggttacgc
      541 gtacggctgt ccggaatttc agccgaatat cattcatccc tgatattacc gggtcatcac
      601 g
//
LOCUS       DAPHJE010000351          589 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPHJE010000351 DAPHJE010000000
VERSION     DAPHJE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.357"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            501..>589
                     /locus_tag="RH222_004659"
     CDS             501..>589
                     /locus_tag="RH222_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799541.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFL"
BASE COUNT          188 a          124 c          106 g          171 t
ORIGIN      
        1 actggctggt ttccgttaat ggattaaata tctattattc tcccgagatt aaaactatca
       61 aatcaatgag taaccgatac agcctcacaa attctttcag ataattaacc cgtcactgcg
      121 gcacgttctc tcataaggcg tgcctgcagt gtaatccagt atgtatccgg catagttgac
      181 ggtttaatag aactcaattc ctctcccagg tcaccaacaa cgatatcttg tataacaccc
      241 caggagatac gacattatga tgagatgcga tatgctcctc agcaccaatc aacgcccaga
      301 ctgggccagt cttgagatca gactcattcc cggaactgac ttcaggcgac atatatcaat
      361 gacgttggtg caataaaaga tagttgatgt aatacatcta ttcaaatttg tgatacatgt
      421 caaaaaattg gttgaccaaa ctcgttattt atataagggc acttacgaag tgcactcttt
      481 tttaaagcga ggaagtacca atgaaagaga ataaagtaca gcaaatcagt cataaactga
      541 ttaatatcgt tgtttttgtc gcaattgtag aatacgccta tttatttct
//
LOCUS       DAPHJE010000352          587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPHJE010000352 DAPHJE010000000
VERSION     DAPHJE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.358"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            186..261
                     /locus_tag="RH222_004660"
     tRNA            186..261
                     /locus_tag="RH222_004660"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Ile,seq:cat)
BASE COUNT          165 a          105 c          135 g          182 t
ORIGIN      
        1 gtgctaatta ttaacatttg cttgttgact gcgagtatcg actttgcgaa tggaagcggt
       61 gtcgggtttt atgtcgcgtg taatgacgag caaggatgaa tgagaacgca taaaaatctg
      121 ttatttacat catatgatca tcatgtttat tgcgtggttt ataagtattg ttattgcaca
      181 ctcaaggccc tttagctcag tggttagagc aggcgactca taatcgcttg gtcgctggtt
      241 caagtccagc aagggccacc aaatcattat ttacgtatca ggaagagtta agggtacaag
      301 gggtactcga tggcggtcta tatttcatga ctaacgccat ttcaaggaag aagtgcggtg
      361 ggttgtttcg aatgagggct ggtgttttgg tacgtggcac gccactgcaa tgtatttatc
      421 catcagtact cattcaaaag tacctggaaa aaatgcccgc tatgccaagc gggcaaattg
      481 cttacatgtg gaattaagta attaacgata aagccagagt attatcattt attatacccc
      541 ttaatttaat caggttaatt actcaaatca ctgaactatt ctctgtt
//
LOCUS       DAPHJE010000353          578 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPHJE010000353 DAPHJE010000000
VERSION     DAPHJE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.359"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            40..>578
                     /locus_tag="RH222_004661"
     CDS             40..>578
                     /locus_tag="RH222_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDV3799542.1"
                     /translation="MTQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGG
                     TGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAA
                     GRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCK
                     MKCSGNNIGRLIRFVFTGV"
BASE COUNT          177 a          100 c          148 g          153 t
ORIGIN      
        1 aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga tccgattggg
       61 ctgaaggggg gatggaattt ttatcagtat ccgttgaatc cgatctcaaa tatagatcca
      121 ttaggattag aaacactaaa atgcattaag ccactgcatt caatgggcgg aactggtgaa
      181 agaagcggtc cagatatatg ggggaatccg ttctatcatc aatatctttg tgtcccagat
      241 ggtaaagggg actatacttg tggtggccaa gaccaacggg gagaatcaaa aggagatggt
      301 ctatgggggc caggtaaagc aagtaatgat acaaaagaag ctgctggccg ttgtgacctc
      361 gttgaaaccg ataatagttg tgtggagaac tgtttaaaag ggaagtttaa agaggtaagg
      421 ccgcgttatt ctgtattgcc tgatatattc acacctataa atttagggct atttaaaaac
      481 tgccaagact ggtctaatga ttctttagaa acatgtaaga tgaagtgctc cggaaataac
      541 attggacgtc ttattagatt tgtattcacc ggagtgat
//
LOCUS       DAPHJE010000354          578 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPHJE010000354 DAPHJE010000000
VERSION     DAPHJE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.360"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..567
                     /locus_tag="RH222_004662"
     CDS             25..567
                     /locus_tag="RH222_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3799543.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT          172 a          116 c          134 g          156 t
ORIGIN      
        1 tacgaagaga tcaggatgat gacgatgaaa aaaagtgtat tgacggcgtt tataactgtg
       61 gtatgtgcaa cgtccagcgt tatggcagct gatgataatg ctatcacgga tggcaaagta
      121 acatttaatg gtaaagttat tgctccagct tgtaccctgg tagctgcgac gaaagattca
      181 gtagtcacat tgccaaatgt cagcgccacc aagttgcaaa ctaatggagc agtctctggc
      241 gttaaaactg atgtaccaat tgccttagaa ggctgtgatg ttactgtaac taaaaatgcg
      301 acgtttacct ttagtggtac ggctgacggc gtgcaaccta cagcatttgc caaccaggct
      361 accaccgatg cagcgacgaa tgttgctcta caaatgtatc tgccagatgg ttctacatcg
      421 gtaactcccg gtaccgaaac gtcgaatatt cagttagcgg attctgctga gcaaacagtg
      481 acctttaaag ttgattatat cgctacgggg aaagcgactt ctggtaatgt gaatgcggta
      541 acaaatttcc atattaacta ttattaatag aactcatt
//
LOCUS       DAPHJE010000355          576 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPHJE010000355 DAPHJE010000000
VERSION     DAPHJE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.361"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..304
                     /locus_tag="RH222_004663"
     CDS             <1..304
                     /locus_tag="RH222_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV3799544.1"
                     /translation="DNHCLSPSKISRELQFTRTNITRITDFLEKAGYIERTDSREDRR
                     AKKISLTSDGLSFIQNVNIAQSLHLKEIWSPLTRAECELFEDINKKLLTHIADVCP"
BASE COUNT          208 a          112 c          120 g          136 t
ORIGIN      
        1 agataatcat tgtctttcgc cctcaaagat aagcagagaa ctccagttta ccagaacaaa
       61 cattacccgc attacagatt ttttagaaaa agcagggtat atagaaagaa ctgatagcag
      121 agaggatcgc cgtgctaaaa aaatcagtct gacatcagat ggtctgtcct ttattcagaa
      181 cgtcaatatt gcacaaagtc tgcacctgaa agaaatctgg agcccgctga cccgtgcaga
      241 gtgtgaactg tttgaagaca ttaataaaaa attactgacg catattgctg atgtgtgtcc
      301 ctagagcatg aagagtttga ggaaactgaa gagcttcaag caatgaatca tataataagc
      361 aggcataaga gcgacataac tacaggcgga aacagcgata caagaaaact actggtttag
      421 gtcacaatgc agcaaaatga caaactcata cgacggttct tctgaatgat gcggtgccta
      481 acccgcaaat atcagcatga ttcaccgtag aacttactga tacaagataa cgtaatgatt
      541 gagcttatct catatggggt aacgcgcaat aaaaat
//
LOCUS       DAPHJE010000356          575 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPHJE010000356 DAPHJE010000000
VERSION     DAPHJE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.362"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            118..>575
                     /gene="csgD"
                     /locus_tag="RH222_004664"
     CDS             118..>575
                     /gene="csgD"
                     /locus_tag="RH222_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV3799545.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTES"
BASE COUNT          172 a          118 c          116 g          169 t
ORIGIN      
        1 catttagggc tgatttatta ctacacacag cagtgcaaca tctgtcagta cttctggtgc
       61 ttctatttta gaggcagctg tcaggtgtgc gatcaataaa aaaagcgggg tttcatcatg
      121 tttaatgaag tccatagtat tcatggtcat acattattgt tgatcactaa accgtctttg
      181 caagcgacag ctctcttgca gcaccttaaa caatcgctgg caattacagg aaaattacat
      241 aatattcaac gttctctgga cgatatatct tcaggctcta ttattcttct ggatatgatg
      301 gaagcagata aaaagcttat ccattattgg caggatactt tgagcaggaa aaacaacaat
      361 atcaaaatat tgttgctaaa tacgcctgaa gattacccgt accgcgacat tgaaaactgg
      421 cctcatatca acggcgtttt ttatgccatg gaggatcaag aacgtgttgt caatgggttg
      481 caaggcgtcc tgcgcggcga atgctacttt acgcaaaagc ttgccagtta cctgattaca
      541 cattctggta actatcgtta taacagcacg gaatc
//
LOCUS       DAPHJE010000357          565 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPHJE010000357 DAPHJE010000000
VERSION     DAPHJE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.364"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>565
                     /locus_tag="RH222_004665"
     CDS             <1..>565
                     /locus_tag="RH222_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDV3799546.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATS
                     FLVDFLPILRRTLTRTGFVIDHIHYYA"
BASE COUNT          100 a          190 c          175 g          100 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa ccgccggccg
      421 cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca cgcgctactt
      481 cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc ggctttgtca
      541 tcgaccacat ccactactac gccga
//
LOCUS       DAPHJE010000358          545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPHJE010000358 DAPHJE010000000
VERSION     DAPHJE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.365"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          188 a           94 c          101 g          162 t
ORIGIN      
        1 aaattagctt tattgaggac agctttacct aaagctgaaa agtgctaaat atcaaacttt
       61 catcccataa gctatgatat agcactcact ttattgataa attccgaggt attcatgaaa
      121 ggtcagatcg atatcgaact ccaaagattg atacgggcgc ggtataacac cgcggtagaa
      181 aattggagtg aatggaaatg gttagaaatc gggcatggcg tcgtcatcat atatctcggt
      241 taaaatctaa gagaaaaaca tatcgaattg cgttggctaa aactgaaaaa gttgtaggga
      301 aaatttactg caccccttgt gcttgttctt gttatttatg tggtaatcaa agaatttatc
      361 acggagtgaa cattcaagag caacgagctt tgctgcgttt tactgattga cttataagat
      421 attaatcttt tcgataataa aaaggctcca atccctggag cctaaattgg aaaagctctc
      481 attaaaatta ttattttcaa ctacatcaca gaatattttc ccgtgacata taaccaacaa
      541 cagat
//
LOCUS       DAPHJE010000359          522 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPHJE010000359 DAPHJE010000000
VERSION     DAPHJE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.366"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>522)
                     /locus_tag="RH222_004666"
     CDS             complement(<1..>522)
                     /locus_tag="RH222_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV3799547.1"
                     /translation="IRLLSGPLNGCEYEILNGRLLVIIGNDVSLGRSDAFSELPENTI
                     VVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLNQQIEVLGLKFAVKEKNE
                     VWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFIAFYIVNASNDPQLRHID
                     KILVNKNRNYEIL"
BASE COUNT          178 a           96 c           76 g          172 t
ORIGIN      
        1 ataaaatttc ataattcctg tttttgttta caagaatttt atcgatatgt ctcagctgcg
       61 gatcattact ggcattaaca atataaaaag caatgaaaat gatagcaaaa agaaaaaaaa
      121 gcattacata cttaaacagc ttactgctaa agaaatgttg ttttgtagaa ataatgttat
      181 tttcaataat gcctggcaat gaatactgcc aaacttcatt tttttctttt acagcaaatt
      241 tgagacctaa aacttctatt tgctgattca atgttaacgt tctatcttca ggttcttgtg
      301 ctgttaattc tctgaatgtt actaccagat cagggtcggt agtaattatt atctcaaaac
      361 tacctgtaag ctcgccataa ggaacgacta ttgtgttttc tggtaactca gaaaaagcat
      421 cacttcttcc taacgaaaca tcattaccaa tgataacaag tagacgccca ttaagtattt
      481 catactcgca tccattcaat ggtccgctca acaaacgaat gg
//
LOCUS       DAPHJE010000360          522 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPHJE010000360 DAPHJE010000000
VERSION     DAPHJE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.367"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>522
                     /locus_tag="RH222_004667"
     CDS             <1..>522
                     /locus_tag="RH222_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799548.1"
                     /translation="INTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQPINSTFSGE
                     GYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESISSTPLTRQAQ
                     EILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSVQTMFSDTNF
                     IAEQRADLPTNIK"
BASE COUNT          197 a          114 c           89 g          122 t
ORIGIN      
        1 gataaataca tatcacggcg atatttataa ccgcacaaca gataccgaca atgttaaaac
       61 accagacaca ccgatgtggc cttgtgataa tcgggaagaa caacaaccca ttaattccac
      121 attctctgga gaaggataca accctgaaca atatgattta gcgcggcaac acctcccaca
      181 aatttatgcg tgccgcacga acaccaaata tacagatgca gactactcaa aagttgtggc
      241 tcaacttgta agtcttatta ctaacattga atcgataagt tcgacaccac ttacgcggca
      301 ggcacaggaa atacttaatc aaattaataa cattagatac gaaaagaaca aaagtgctga
      361 atgtcgaatc attgttgtcg ctaatcctaa acccgacaag gcaatcataa ctaaaattag
      421 cgtagaagag ggaataccag taagattcag tgtccaaacc atgttttcag atactaattt
      481 tattgctgaa caacgagctg acttaccgac taatataaaa ga
//
LOCUS       DAPHJE010000361          518 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPHJE010000361 DAPHJE010000000
VERSION     DAPHJE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.368"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>518
                     /locus_tag="RH222_004668"
     CDS             <1..>518
                     /locus_tag="RH222_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3799549.1"
                     /translation="PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQR
                     YVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLIL
                     DNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPC
                     KLLLTTPGIDAR"
BASE COUNT          140 a          126 c          118 g          134 t
ORIGIN      
        1 ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca atccgtacat
       61 aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag ccacatcaaa
      121 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcacgcctcc
      181 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
      241 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaaactg
      301 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga tggtaaaccc
      361 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
      421 ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt cgatccttgc
      481 aaactgttgc tgacaacccc aggtattgat gcacgtaa
//
LOCUS       DAPHJE010000362          512 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPHJE010000362 DAPHJE010000000
VERSION     DAPHJE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.369"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>512)
                     /locus_tag="RH222_004669"
     CDS             complement(<1..>512)
                     /locus_tag="RH222_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3799550.1"
                     /translation="VNDVEEFRKDLNLPSNRKIILYSGNVGDKQGLEIIPDIASNPAF
                     SDSVFLIVGEGAGKKRLEEKVLSSQLTNVLFRPLQPYNKLPLLLSMADCHLVIQKRGA
                     ADAVLPSKLTNILAVGGNAIITAEIHTELGQLTTNYPGIAVRVEPESDVSLTEGLIHA
                     LSLPKFNSIA"
BASE COUNT          161 a          101 c           85 g          165 t
ORIGIN      
        1 cagctatact attgaattta ggaagtgata atgcatgaat taatccttcc gtaagtgaaa
       61 catcactctc tggttcaact cgtactgcaa taccaggata gttagttgtt aactgaccta
      121 gttctgtatg aatttcagct gtaataatag cgttaccacc aacagcaagg atgtttgtaa
      181 gttttgaagg taacaccgca tcagctgcgc ctcttttctg aataactaag tggcaatcag
      241 ccatacttag caatagcggt aatttattat aaggctgtaa gggacgaaat aatacatttg
      301 ttaattgact gctgagtact ttttcttcga gacgtttttt accagcacct tcacccacga
      361 tcaaaaaaac actatctgaa aatgcaggat ttgaagcaat atcaggtatt atttctaatc
      421 cttgtttatc acctacattt cccgagtaaa gaataatttt tcgatttgaa ggcaagttca
      481 aatctttcct aaattcctca acatcgttaa cc
//
LOCUS       DAPHJE010000363          502 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPHJE010000363 DAPHJE010000000
VERSION     DAPHJE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.370"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..343)
                     /locus_tag="RH222_004670"
     CDS             complement(<1..343)
                     /locus_tag="RH222_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3799551.1"
                     /translation="MKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVAL
                     AVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPV
                     PDEQLSSEDKFI"
     gene            complement(340..>502)
                     /locus_tag="RH222_004671"
     CDS             complement(340..>502)
                     /locus_tag="RH222_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3799552.1"
                     /translation="IVLIFDFIAEYFLFMKDMKMDKQEVKREYKKQEGNPEIKSKRRE
                     RIRKFFLSN"
BASE COUNT          147 a           93 c           80 g          182 t
ORIGIN      
        1 caatgaattt atcttctgaa gagagctgtt catctggaac aggttgtcca gcattctcca
       61 catcttcaag ccacagcaga agacgtaata tttcatctat attttcgaag ctaacataat
      121 cgtagcgacg atgggtagca taaatttttc gtgctaattt tttatctgtg ataattggta
      181 ttccgatttc ctttgcatat ttccttacgg ctaaggctac ctcattagtt tctcttacag
      241 aaatcaatgg aataggtgaa agatgtggct taaagtatat tcctattgca atgtgagtag
      301 ggttggcaat catcaaacga ctattactga catcagactt caattgctca gaaagaattt
      361 cctgatgcgc tctctgcgtt tagacttaat ttctggattt ccttcttgct tcttgtattc
      421 tcttttaact tcttgtttat ccattttcat atctttcata aataaaaaat attcagcgat
      481 aaaatcaaaa attaagacaa tt
//
LOCUS       DAPHJE010000364          501 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPHJE010000364 DAPHJE010000000
VERSION     DAPHJE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.371"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..359
                     /locus_tag="RH222_004672"
     CDS             69..359
                     /locus_tag="RH222_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV3799553.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANCSRGKNL"
     gene            383..>501
                     /locus_tag="RH222_004673"
     CDS             383..>501
                     /locus_tag="RH222_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV3799554.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNL"
BASE COUNT          175 a           90 c           95 g          141 t
ORIGIN      
        1 ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa ttaaaaggat
       61 tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta gctgcgaacg
      121 tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt tatggcaagg
      181 ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa acttatgccc
      241 gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt ggtcactggg
      301 aatatgattt taaaggctat aacgatgaag ccaactgctc gcgcggcaag aatctctgat
      361 tgagttaagt gcccatcaca atatgatcac tgaccttata ttacacaatc atcccaggat
      421 gaaaacaatc actttaaacg acaaccatat tgcacattta aacgccaaaa acactacaaa
      481 actggaatat ttaaacttaa g
//
LOCUS       DAPHJE010000365          500 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPHJE010000365 DAPHJE010000000
VERSION     DAPHJE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.372"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>500
                     /locus_tag="RH222_004674"
     CDS             <1..>500
                     /locus_tag="RH222_004674"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014838955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HDV3799555.1"
                     /translation="AIESIRGLRTSLHFAMMEARNNILMISSASPNAGKTFVSSNLAA
                     VISQTGKKVLFIDADMRKGYTHKLFKASNENGLSDYLSGKVELQNIIQKIPVGDFDFI
                     PRGIVPPNPAELLMHRRFGELTNWANEHYDIVIMDTPPVLAVTDAAIIGNYVGTTLLI
                     VRFEQNT"
BASE COUNT          165 a           81 c          103 g          151 t
ORIGIN      
        1 gcaattgaat caataagagg attacgcacc agccttcatt ttgcaatgat ggaagctaga
       61 aataatatat taatgatctc tagtgctagt ccgaatgctg gtaaaacttt tgttagttca
      121 aatttagcag cagttatatc tcagactggc aaaaaagtcc tgtttattga tgcggatatg
      181 cgaaagggat acacgcataa gctatttaaa gcgagtaatg aaaatggatt atcagattat
      241 ttatctggga aagtcgaact gcaaaatatt attcagaaaa tccctgtagg agactttgat
      301 ttcataccaa gaggcattgt tccacctaat cctgcagagt tgttaatgca taggcgattt
      361 ggtgaattaa caaactgggc taatgagcat tacgatatcg taattatgga caccccgcca
      421 gttttagcag taacagatgc agcgattatt ggcaattatg tagggactac tttacttata
      481 gttcgttttg aacagaatac
//
LOCUS       DAPHJE010000366          499 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPHJE010000366 DAPHJE010000000
VERSION     DAPHJE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.373"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>499
                     /locus_tag="RH222_004675"
     CDS             <1..>499
                     /locus_tag="RH222_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3799556.1"
                     /translation="RGENTKAQTLEYYLNYILTRAESSALVAVVASIVCAFHEKTFNV
                     AKILFRTKEFFIFDTSRMMQDQTHKTQLTLLKNPLINRIDELHENERISTCDKKHRKL
                     SLENIALHYQFFRAEELSERETEKRLQEIWKILDAHYTDLPDKERESHQDKTWRLYLA
                     RMDKR"
BASE COUNT          191 a           92 c           93 g          123 t
ORIGIN      
        1 aaagaggaga aaacacaaaa gctcaaacac ttgaatatta tctaaactat atcctaacta
       61 gagctgaatc ctctgcactc gtggcagttg tcgcaagcat tgtatgtgcg tttcatgaga
      121 aaacatttaa tgttgctaaa attctattca ggacaaaaga gtttttcatc tttgacacat
      181 ctcgaatgat gcaggatcaa acacataaga ctcagcttac tttgctaaaa aaccctctca
      241 tcaatcggat agatgaactt cacgaaaatg aaaggataag cacctgtgac aaaaaacatc
      301 ggaaactatc attagaaaac attgcgcttc actaccagtt ttttagagca gaggaactaa
      361 gcgaaaggga aacagagaaa aggctccagg aaatttggaa gattcttgat gctcactata
      421 cagatttacc agataaagaa cgagaaagtc atcaagataa aacgtggcgt ttatatttgg
      481 cccgtatgga caaaagaaa
//
LOCUS       DAPHJE010000367          492 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPHJE010000367 DAPHJE010000000
VERSION     DAPHJE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.374"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>492
                     /locus_tag="RH222_004676"
     rRNA            <1..>492
                     /locus_tag="RH222_004676"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          122 a          119 c          148 g          103 t
ORIGIN      
        1 acaggtgctg catggctgtc gtcagctcgt gttgtgaaat gttgggttaa gtcccgcaac
       61 gagcgcaacc cttatccttt gttgccagcg gtccggccgg gaactcaaag gagactgcca
      121 gtgataaact ggaggaaggt ggggatgacg tcaagtcatc atggccctta cgaccagggc
      181 tacacacgtg ctacaatggc gcatacaaag agaagcgacc tcgcgagagc aagcggacct
      241 cataaagtgc gtcgtagtcc ggattggagt ctgcaactcg actccatgaa gtcggaatcg
      301 ctagtaatcg tggatcagaa tgccacggtg aatacgttcc cgggccttgt acacaccgcc
      361 cgtcacacca tgggagtggg ttgcaaaaga agtaggtagc ttaaccttcg ggagggcgct
      421 taccactttg tgattcatga ctggggtgaa gtcgtaacaa ggtaaccgta ggggaacctg
      481 cggttggatc ac
//
LOCUS       DAPHJE010000368          490 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPHJE010000368 DAPHJE010000000
VERSION     DAPHJE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.375"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>490
                     /locus_tag="RH222_004677"
     rRNA            <1..>490
                     /locus_tag="RH222_004677"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          122 a          112 c          163 g           93 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc
//
LOCUS       DAPHJE010000369          488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPHJE010000369 DAPHJE010000000
VERSION     DAPHJE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.376"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..458
                     /locus_tag="RH222_004678"
     CDS             135..458
                     /locus_tag="RH222_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV3799557.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          105 a          129 c          114 g          140 t
ORIGIN      
        1 aatcaaaaaa cagcccccct atttctgaca cctacagatg gcaagaaata gcgcctgcca
       61 ggcgtctttt ccggccattg tcgcagcact gtaacgcgta aaatagtgct ttctcttact
      121 cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt
      181 gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgtacggt
      241 tagtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc agtcgcggga
      301 tcgccgatcg cgcatgccag caggaaggct atacccacgc ggtccccttt ggtcagccag
      361 taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc actttgtcat
      421 atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca gcgaggggat
      481 cttcgctg
//
LOCUS       DAPHJE010000370          471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPHJE010000370 DAPHJE010000000
VERSION     DAPHJE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.377"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>471)
                     /locus_tag="RH222_004679"
     CDS             complement(<1..>471)
                     /locus_tag="RH222_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDV3799558.1"
                     /translation="MDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECK
                     EIGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQLTQSNFR
                     EYQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNMTAVMFDHVRISTGNFKD"
BASE COUNT          161 a           92 c           70 g          148 t
ORIGIN      
        1 caatctttaa aattccctgt cgatattcgc acatgatcaa acataacagc tgtcatattc
       61 gatttaataa atgatatttc tttggcagac agaaactctg ctctggtgaa gtgcgaagcg
      121 taaagataac aattatcgaa gtttgaattt ctaatctgat attcgcgaaa attactctga
      181 gtaagttgac tctgcgagaa gtcaactgta tcaagaatat ttttgctgaa atcgacacat
      241 tcaccactcg ctttgataaa aatcacattg tgtaaacgtc gacatctaaa tatgacatta
      301 tcaactattg cagtagtaaa attgcaatcc ccaatttctt tacattcgaa tgttacatgt
      361 tgtagatttg atgataaaaa agacaccttc gatataacgt cacctttaaa atgacaactt
      421 gttaaatcca aatcagacag gtcgatatac ccctgcgcat ctttatccat c
//
LOCUS       DAPHJE010000371          471 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPHJE010000371 DAPHJE010000000
VERSION     DAPHJE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.378"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..328
                     /locus_tag="RH222_004680"
     CDS             <1..328
                     /locus_tag="RH222_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDV3799559.1"
                     /translation="GDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGL
                     SYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAI
                     DKLKRS"
BASE COUNT          139 a           75 c          127 g          130 t
ORIGIN      
        1 tggagatgtc agaaactggg ccgcatttgc aataaatatc aataaatatg ataatagtga
       61 tattagggat tgttttgtgg agatgcttca ggataaaaat gaggaagtcc gtattgaagc
      121 aataatcgga ctttcctaca gaaaagataa aagggtttta tctgttttat gcgatgagtt
      181 aaaaaaaaat actgtttatg atgatatcat tgaagctgcg ggtgaattag gtgataaaac
      241 gctacttcct gttttagata ctatgttgta caagtttgat gacaatgaaa ttataacttc
      301 cgctattgat aagctgaagc gttcatagcg tgagttgcct atgcacagtg ggggattccc
      361 gccggcacgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct ggcatcgtgc ggcctgaccg tggagcagat g
//
LOCUS       DAPHJE010000372          432 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPHJE010000372 DAPHJE010000000
VERSION     DAPHJE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.379"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          108 a          113 c           73 g          138 t
ORIGIN      
        1 tcatattcga ctcctgtgat cttccgccaa aatgcctctc ctgacgttct ccaacccctt
       61 gtaatactgt caatccagat attttctact gctcaatatt caatcggctt caggaatacc
      121 tggtctaagg ttttgtgatg gacgatgagc ggctgaagaa tccgcctgtc ggttcttctg
      181 tcctcgaacg catccgcgat attcacgcca gtatgcgccg tgtttatttg caggtgattt
      241 gcccccctgc tgactctcag ccaactgtat cgccattaag gtgaagatgt tgttttactc
      301 gccccagaac accctttttt atacgactat tctatttctt taaccgctgg cgaaaaaaac
      361 tgactataaa cttcaccaat aatcagactg ccatcaagat taatttgttc atttaatata
      421 tcaaatcaac tt
//
LOCUS       DAPHJE010000373          425 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPHJE010000373 DAPHJE010000000
VERSION     DAPHJE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.381"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            101..292
                     /locus_tag="RH222_004681"
     CDS             101..292
                     /locus_tag="RH222_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDV3799560.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          103 a          111 c           80 g          131 t
ORIGIN      
        1 actgacgctt ttttacataa cgaagaattg accattttgt cctgttgtgc cttaatgtaa
       61 gtaccgtcca cagcgtggga catacttcaa ggaacctttt gtgagtcagg caaccagtat
      121 gcgaaaacga caccgattta acagtcgcat gacccgtatc gtactgctca tcagctttat
      181 cttcttcttt ggccgtttta tctactcgtc cgtcggtgcc tggcagcacc atcagagcaa
      241 aaaagaagct cagcaatcca cactctccgt cgaatcaccg gtacaacgtt agcggttacc
      301 ttctccactt tcacagaacc taacggcact tcgctgtcgg atgcttttgc tctttggaat
      361 tatcaaagcg gcagatattc tttcatctta aattttacgt ctttgtcctg actgatgttt
      421 atcct
//
LOCUS       DAPHJE010000374          422 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPHJE010000374 DAPHJE010000000
VERSION     DAPHJE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.denovo.382"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          145 a           85 c           54 g          138 t
ORIGIN      
        1 ccaattctaa caaaacacta tgactcttaa aatgctcacg aagaaactca gggacatttg
       61 tgatagtctg tttacaaact atattcccat ttaaatctct gtctatgaga acaaaatact
      121 gcaaatccat taaaacattt attaatgttc cccgactata ttttagttta gtttgcagtt
      181 catccaagct aatggttttg aacgagttta caacatcaag aattaacaaa accattttcg
      241 gagatgttga tggcatatat gaccatggta taactggaag gtcatttcct ttcaagtaat
      301 ctcgaaaaac gtcccaatac actgataatt tttcacctgt tttaattacc atcctatctg
      361 aaattagctc taaaacagta tcactcccaa actctttagt aacttcatat tgactaatcg
      421 ga
//
LOCUS       DAPHJE010000375        17547 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.guided.093, whole genome shotgun sequence.
ACCESSION   DAPHJE010000375 DAPHJE010000000
VERSION     DAPHJE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.guided.093"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="RH222_004682"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="RH222_004682"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV3799561.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="RH222_004683"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="RH222_004683"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV3799562.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="RH222_004684"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="RH222_004684"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV3799563.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="RH222_004685"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="RH222_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV3799564.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="RH222_004686"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="RH222_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV3799565.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="RH222_004687"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="RH222_004687"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV3799566.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="RH222_004688"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="RH222_004688"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV3799567.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="RH222_004689"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="RH222_004689"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV3799568.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="RH222_004690"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="RH222_004690"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV3799569.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="RH222_004691"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="RH222_004691"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV3799570.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12901..14034
                     /locus_tag="RH222_004692"
     CDS             12901..14034
                     /locus_tag="RH222_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV3799571.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="RH222_004693"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="RH222_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV3799572.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="RH222_004694"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="RH222_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3799573.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="RH222_004695"
     CDS             complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="RH222_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV3799574.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="RH222_004696"
     CDS             complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="RH222_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV3799575.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15579..16145)
                     /gene="efp"
                     /locus_tag="RH222_004697"
     CDS             complement(15579..16145)
                     /gene="efp"
                     /locus_tag="RH222_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV3799576.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16187..17215
                     /gene="epmB"
                     /locus_tag="RH222_004698"
     CDS             16187..17215
                     /gene="epmB"
                     /locus_tag="RH222_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV3799577.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4368 a         4601 c         4514 g         4064 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgta
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtaaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactact taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccttg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacct gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgac aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggga cgcttactgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttgcgtaat caattaaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 ctttgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa tctgctcggc gaagcgctgc gggcgcaggt ggcacggctg ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtt ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc actgtatctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcgccactgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc acaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcatgtt atccgccgct ggatgctgat ccaacgccgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgtgcgcgt ggcgaagagg
     5281 aagcacatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggtt
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 caatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttacaggaa attttcgcca actttatctc tggcctgatc atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttactggt agcgtgacga
     5881 aaattaatac ccgcgcaacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaagggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc catgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctgaat ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccagct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc accgtcgcga tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgccgatg cggtaccgcc accaaacgaa accagcagaa tgaagatagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaatg ctttcggcgt cccctggatg atggctttca acggtgaata gcaaagcgta
     7021 aagaacgcac cggtataggc gaggaacatc gaaagtccag taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcat tacccagcgt catcccaagg
     7141 ctcttcatca gcacataggt aatattgccg aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacgcc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatgg caaataccac aaacgatagc
     7381 attgccagac cggactgata acccgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat ccccttagaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccgg caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagatacca
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaaacc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatttttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataacac
     8041 gtgcccactg aactgcactg cgcaacattg agcaggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttagcgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttattacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcgtc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttacc aatatttggc tctacgccaa aggtaaacag caattgtagc aacgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgccact tcccgcagtt
     8701 gcgttttgtc ggcagagagt gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactccag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaatca aacagcggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggtactta
     9481 ccctgaagta cgtggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccgaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtggggat gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcctgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggcctg gcaatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgcgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagttacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggca aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctattgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 actggggctg tttccgggtg atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aattcacgtg cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtatcgtta caatctaacg
    12841 tatcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atcttgcgac ctacaccgct ggcggcctgc cattgcaggt gccggatgaa
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt acctccccca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggtaagg cagtgcatgt ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag
    13621 tcgaccattg aagatatggc ccgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg gcaataaaat tgcactggca gcacaccctg taaaagcgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacagggg caaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatagtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtaacag tagtgaataa
    14221 tttcgacgca aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgttt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagtctg cgtgatgacg gcggcctgaa
    14341 tgtcatcaat aaaggctata accctgacag agaaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa cacgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgtcattg cacttgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatggc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctaccg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac tatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacgg ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt tgtgactatc gcaaaaaaac aaacggcacg
    15121 acataactgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc
    15181 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
    15241 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
    15301 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
    15361 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgggag
    15421 atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg
    15481 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
    15541 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
    15601 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
    15661 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
    15721 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
    15781 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcgttg
    15841 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
    15901 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
    15961 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
    16021 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
    16081 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
    16141 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
    16201 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
    16261 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
    16321 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
    16381 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
    16441 cgcgtccgga ttctccaccg acccgctgga agaacagcac agcgtagtgc ctggtttgtt
    16501 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
    16561 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
    16621 gactgctctt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
    16681 cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat
    16741 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
    16801 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
    16861 catcaatcat gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg
    16921 tgtcggtgtc accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
    16981 aacgctggca aacctgagta atgcgttgtt cgatgccggc gtaatgccgt attacctgca
    17041 tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
    17101 gattatgcgt gagttgctga cactggtgtc gggatatctg gtgccgaaac tggcgcgaga
    17161 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
    17221 taaacaggga agtcaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
    17281 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
    17341 tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaactggga aatttaatct
    17401 ttgcccgttg cataaccttt aattattcat atacattgct gttgttactc tttatataac
    17461 taacaccaat aattaacctt tcttttctgt ttatatagag tgagtacggc tatttatgtt
    17521 attttaacaa ctattactac caagccc
//
LOCUS       DAPHJE010000376         9132 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.guided.151, whole genome shotgun sequence.
ACCESSION   DAPHJE010000376 DAPHJE010000000
VERSION     DAPHJE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.guided.151"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(206..1918)
                     /locus_tag="RH222_004699"
                     /pseudo
     CDS             complement(206..1918)
                     /locus_tag="RH222_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1890..2675
                     /gene="lafU"
                     /locus_tag="RH222_004700"
     CDS             1890..2675
                     /gene="lafU"
                     /locus_tag="RH222_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV3799578.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2746..3801
                     /gene="dinB"
                     /locus_tag="RH222_004701"
     CDS             2746..3801
                     /gene="dinB"
                     /locus_tag="RH222_004701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV3799579.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3798..4250
                     /locus_tag="RH222_004702"
     CDS             3798..4250
                     /locus_tag="RH222_004702"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3799580.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
     gene            4569..4775
                     /locus_tag="RH222_004703"
                     /pseudo
     CDS             4569..4775
                     /locus_tag="RH222_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            4771..5123
                     /gene="prfH"
                     /locus_tag="RH222_004704"
                     /pseudo
     CDS             4771..5123
                     /gene="prfH"
                     /locus_tag="RH222_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5180..6637)
                     /gene="pepD"
                     /locus_tag="RH222_004705"
     CDS             complement(5180..6637)
                     /gene="pepD"
                     /locus_tag="RH222_004705"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV3799581.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            6898..7356
                     /gene="gpt"
                     /locus_tag="RH222_004706"
     CDS             6898..7356
                     /gene="gpt"
                     /locus_tag="RH222_004706"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV3799582.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7448..8692
                     /gene="frsA"
                     /locus_tag="RH222_004707"
     CDS             7448..8692
                     /gene="frsA"
                     /locus_tag="RH222_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV3799583.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            8750..>9132
                     /gene="crl"
                     /locus_tag="RH222_004708"
     CDS             8750..>9132
                     /gene="crl"
                     /locus_tag="RH222_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV3799584.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEP"
BASE COUNT         2156 a         2374 c         2434 g         2168 t
ORIGIN      
        1 tgcctgatgc gacgctgacg cgtcttatca tgcctacaaa cctgtgctgg atcggtaggc
       61 cggataaggc gcttacgccg catccggcgg ttgtgcgctg gtgccggatg cgcttgcgct
      121 tatccggcct acgaatcgtg cctgaacagt aggacggata aggcgttcac gccgcatccg
      181 gcagttgtgc accaatgccg gatacttaca tcaacgcccc cattatcttc aactccagct
      241 cgtccggcac ttcgttatac gacagcacat gcagccccgg cgcaaacaac cttgcataac
      301 gcgccagcaa agggcgcagc tgaggcggca ccagcagcac cggatctttc cccgccgctt
      361 tcatctgctc ttttacctgc ggcatcgtac tctggaactg gttgagcata ttcggatcga
      421 ccggcacgct gtcgagcatc actttcccgc cctgttgcgc ctgattcacc acgttggtca
      481 gcagattttc cagctcatta ttcagcgtat acaccgtcag ctcctgcttg cgaacgaacg
      541 gatgggtaat gcttcgccgc agcgccaggc gcacatcagc tgccagcaga atatgatctt
      601 tggtcaccgc gctactggcg accagcacgg tggcaatggt gacgatatcg cgcagggaaa
      661 cgccttcggt cagcagcgcg cggtacactt tcagcaactg gctgtaattc agcgccgcgc
      721 ttaaatcttc cgccaggcgc ggtgccatcg acgacaaacg gttatgcaac tgcgtaatat
      781 cgtcatagtt aaataaatca ggaatatagc tacgcacaat cttattcaca tgcgtggcaa
      841 tcacgctggc gctgtcgatc acctgatacc ccatattcag cgccttcgct ttctgcgcgg
      901 gctggatcca ggtcaccggc atcccgtacg ccgggtcgtt ccccagcacg ccgtcaattt
      961 cgccgtaggt ttcgctggag ggtagggcca tcagtttatc cgccggaata tccgcttcat
     1021 cagccttaat gccgttgatg aaaatagcgt actggctggg cttgaggcgg aagttttccc
     1081 gaatacggat ctccggcagc agcacgccgt taccgtcgga tatcacctgc cgcacgccgc
     1141 gaatccgctg ggtgagcggg ttgccctgcg ctttatccac cagcgccacc agcttgtagc
     1201 cgaggcttaa gctgataggc tcgatcagcg gtatcgtctc ccaactgacc tgttgctcgc
     1261 tggtttcggt gatggtgcgg gtcagcgttt cgaggctttt ttcttccgcc tccgccgcct
     1321 gcgggcgttt gctcatccgc cagccggtaa agccaagcag ggcgctgaac agcaaaaacg
     1381 gcaggtgcgg catccccggc accactgcca gcacgaacat gatcccggta gcggtgtaga
     1441 gcaccgacgg gcttgccagc agctggtggc gcacgtcatg ggtgatatcg ccgttgtcgc
     1501 tgatgcgggt gacaataatc gccgccgcgg tggagagcag cagggaaggg atctgcgcca
     1561 ccagcccgtc gccgatggtc atcagcacat actgctggaa ggcagcatcg gcgctcaggt
     1621 tgtatttgaa gatcccgata cagacgccgc cgatcaggtt gattgccaga atcatcatcc
     1681 cggcgatggc atccccgcgc acaaactttg acgccccgtc catcgcgccg tagaaatcgg
     1741 cctcgctggc gacatcttta cgccgggttt gcgcctgcgc ctggttgatc aacccggcgt
     1801 taaggtcggc gtcaatcgcc atctgtttgc cgggcatcgc gtccagggta aagcgggcag
     1861 aaacctcgga aatacgctcg gcccctttgg tgacgacgat aaagttgatt gtcaacagcg
     1921 tcagtaaatc cgagcgtgaa agcattatcg ccgcgctgca cgggcagtcg attttcagtg
     1981 gcggcggatt gtcgccgtta aataaaatca gcccgtctca tccgccaaaa cctgccaccg
     2041 tggcggtgcc ggaagagacg gagaaaaaag cgcgagacgt caacgagaaa acagcgctgc
     2101 tgaagaagaa atccgccacc gagcttggcg agctggcaac cagtatcaac accattgccc
     2161 gtgatgcgca tatggaagcg aatctggaga tggagattgt tccccaggga ttgcgcgtgc
     2221 tgattaaaga tgaccagaac cgcaatatgt tcgaacgcgg cagcgcgcag attatgccgt
     2281 tctttaaaac tttgctggtg gagctcgcgc cagtgttcga ctcgctcgat aacaaaatta
     2341 ttattaccgg gcataccgat gcgatggcct acaaaaacaa tatctacaac aactggaacc
     2401 tttcgggcga tcgcgcgctt tcagctcgtc gggtgctgga agaggccgga atgccggaag
     2461 ataaagtgat gcaggtaagc gcaatggcgg accagatgct gctggatgcc aaaaatccgc
     2521 aaagcgcggg caatcggcgt attgagatta tggtgctgac caaaagtgcg tccgatacgc
     2581 tgtatcaata ctttggtcag catggggata aagtggtgca gccgctggtg caaaagctgg
     2641 ataagcagca ggtgctttcg cagcgaatgc gctaaatgct gaatctttac gcatttctca
     2701 aaccctgaaa tcactgtata ctttaccagt gttgagaggt gagcaatgcg taaaatcatt
     2761 catgtggata tggactgctt tttcgccgca gtggagatgc gcgacaatcc cgccctgcgc
     2821 gatatcccta ttgctattgg cggcagccgc gaacgtcggg gggtgatcag caccgccaat
     2881 tatcccgcgc gtaaatttgg cgtacgtagc gctatgccta ctgggatggc gctcaaatta
     2941 tgcccacatc tcaccttgct tccggggcgc tttgacgcct acaaagaagc ctcaaatcat
     3001 atccgtgaaa tcttctcgcg ctacacctcg cgcattgaac cgttgtcact ggatgaggct
     3061 tatctcgatg tcaccgatag cgtccattgc cacggttctg cgaccctcat cgcccaggaa
     3121 atccgccaga cgattttcaa cgagctgcac ctgacggcgt ctgcgggcgt tgcgccggtc
     3181 aagtttctcg ccaaaatcgc ctccgacatg aataaaccca acggccagtt tgtgattact
     3241 ccggcagaag ttccggcatt tctgcaaacc ttaccattgg caaaaatccc cggcgtcggc
     3301 aaagtctcgg cggcaaaact ggaagcgatg gggctgcgga cctgcggtga tgtgcaaaag
     3361 tgtgatctgg tgattctgct taaacgcttt ggcaaatttg gccgcatttt gtgggagcgt
     3421 agtcagggga ttgacgaacg cgacgttaac agcgaacggt tgcgaaaatc cgtcggtgtg
     3481 gaacgcacga tggcggaaga tattcatcac tggtctgaat gtgaagcgat tatcgagcgg
     3541 ctgtatccgg aacttgaacg ccgtctggca aaggtaaaac ctgatttact gattgctcgc
     3601 cagggggtga aattaaagtt cgacgatttt cagcaaacca cccaggagca cgtctggccg
     3661 cggctgaata aagctgatct aatcgccacc gcgcgtaaaa cctgggatga acgccgcggc
     3721 gggcgcggtg tgcgactggt ggggctgcat gtgacgttgc ttgaccctca aatggaaaga
     3781 caactggtgc tgggattatg aataacatac aaataagaaa ctatcagcct ggcgattttc
     3841 agcaactatg cgctattttc attagagcgg ttatgatgac cgccagtcag cattattcac
     3901 cacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
     3961 aatcacaagt gcgggttgca gtcattaatg cacaaccggt tggttttatt tcccgcattg
     4021 aacgtcatat cgatatgtta tttgttgacc ctgaatacac ccgccgcggg gttgccagcg
     4081 ccttgttaaa acctttgatt aagtctgaat ccgaacttac ggtggacgcc agcataaccg
     4141 caaaaccctt ttttgaacgt tatggttttc agatagttaa gcagcagcac gttgaatgcc
     4201 ggggagcgtg gtttactaat ttttatatgc gatataaacc gcaacattaa atccagcttg
     4261 caatgaaaat aacgcccgcc tggtatgtgc gcgttttctt attccacaaa ctgcaaggag
     4321 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
     4381 tttttcttgc cctggattcg tctgccattt cctgattttt aatatttata tactctaaat
     4441 aattcgagtt gcaggaaggt gacaagcgag tgaatcgcca ggagcttact aaagtaagtg
     4501 actggggtga acgaacgcag tcgcagtaca tgcaacttga agtatgacga gtataaatgg
     4561 aatggtacat gggcaaatat attcgtccct tatccgatgc ggtatttacc atcgcatctg
     4621 atgacctgtg gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca
     4681 acatgcagcg cgtagtgggg atgccagatt tacatcccgg acgcggctat ccgattggcg
     4741 cagcgttctt ctctgttggt cgtttttatc cggcaagacg tcgcggtaac ggtgctggaa
     4801 acagaaacgg gccgctactc tgacacgctg cgttcggcgc tggtttctct ggatggcgat
     4861 aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg tccgagtccg
     4921 tatcggtctc atcatgggcg caaaaactgg tttctgggca tgctaacaaa cggctggcgc
     4981 gattgctgat tgcctggaag ctggagcagc agcaacagga aaatagcgcg gcgctgaaat
     5041 cgcagcggcg aatgttccat caccagattg aacgtggcaa cccgcgacgg acatttacag
     5101 ggatggcttt tatcgaagga taatgaagga tgaaactgcc ggaaggcgat taaacgccat
     5161 ccggcagcaa atcaaataat tacttcgccg gaatttcttt cagcagttca gtcagcagtg
     5221 tccagtaatg acctacgctt ttgatgtgaa cttgctcatc cggagagtgt ggaccggtga
     5281 tagttggccc gatagaaacc atgtccattt ccggatacgg ttttttaaac agaccacatt
     5341 ccaggcccgc gtggataatc tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt
     5401 cacgtaccag atgcatcacc ggagaattag cgtccggctg ccagccagga tatgcgcctt
     5461 tcgcttcggt tttcgcgcca gccagtttac ccagcgaatc cagcatgctc accacgtagt
     5521 ctttaccgct gtcgatcagt gaacggatca ggcagtgaat ttctacgtta ttgtcagtca
     5581 tggtcaccac accgacgttc agggaggttt cgaccacacc ttttgccacg tcggaattgc
     5641 ggatcacacc gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa
     5701 tcagagcagc tttatcgttc gctacagagt ccagcaacaa ggccagattc ttctctttct
     5761 ctgccagctc gtttttcagg atgtcctgat aggtattcac cagagatttc agggcgtcga
     5821 ctttatcagc tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg
     5881 tgccaccgtt gaaatcgata aggcgcaggt ccagctcttc cgcatgaccc gccaggaagc
     5941 gcaccagcag tttgttggca ttacccaggc caacgtggat ttcaccgccg gagtgaccgc
     6001 cttttagacc ttttaaggtt aacttgaagg tttcaaagcc agctggaacc gcttcacgat
     6061 ccaaatgcag gttggaggtg aagtcgatac cccccgcgca acccatgtag atttcacctt
     6121 cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccga
     6181 acgcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc gggccgtgaa
     6241 ccacgttttc gtcagccaga accgccagcg cagacgccat accaatgccg ttatctgcac
     6301 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt
     6361 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgca
     6421 agacgaccgg tttacgattt tccatacctg cggtagcagg tttacgaatc aggatattac
     6481 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
     6541 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
     6601 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
     6661 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
     6721 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     6781 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     6841 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     6901 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     6961 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
     7021 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     7081 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     7141 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     7201 gaaatgtatc caaaagcgca ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg
     7261 gttgataatt atgttgttga tatcccgcaa gatacctgga tcgaacagcc gtgggatatg
     7321 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     7381 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     7441 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     7501 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     7561 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     7621 gcgcggcatt gacccacgtg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     7681 tgaacgtacc gatgatgatt tatacgatac cgtgattggc taccgtggtg gcaactggat
     7741 ttatgagtgg gccacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc
     7801 gcaactcagt ggtcgacact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc
     7861 tcatctgaaa ggagatgacc tggccgagca agcgcaggct ttatcaaacc gcgcctatga
     7921 agaggccgcc cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg
     7981 tgcgcccatc accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt
     8041 aatgtgtggt ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt
     8101 tgcgccgcgc ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcttcaaa
     8161 atggaagctc acccaggact ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa
     8221 cgtaccgtgg gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt
     8281 tgccgtgcgt ctggcatacc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc
     8341 ggtagttcat accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct
     8401 tgacgttctg gcgagtcgtt tggggatgca tgatgcttcc gatgacgcgt tgcgcgtgga
     8461 gctgaatcgc tattcattaa aagtgcaagg attgctggga cgtcgctgcc caacgccaat
     8521 gttatcaggc tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac
     8581 ctcatcatct gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt
     8641 tgacaaaggt cttcaggaaa tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc
     8701 taaattttgc caatttggta aaacagttgc atcacaacag gagatagcaa tgacgttacc
     8761 gagtggacac ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg
     8821 tgaaggtaag tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt
     8881 gaaaccggca ccggaagtgc gtgaattctg gggctggtgg atggagctgg aagcgcagga
     8941 atcccgtttt acctacagct accagtttgg tctgttcgat aaagcaggcg actggaagag
     9001 cgttccggta aaagacactg aagtggttga acgactggag cacaccctgc gtgagtttca
     9061 cgagaagctg cgtgaactgc tgacgacgct gaatctgaag ctggaaccgg cggatgattt
     9121 tcgtgacgag cc
//
LOCUS       DAPHJE010000377         3936 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.guided.227, whole genome shotgun sequence.
ACCESSION   DAPHJE010000377 DAPHJE010000000
VERSION     DAPHJE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.guided.227"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            767..1009
                     /locus_tag="RH222_004709"
     CDS             767..1009
                     /locus_tag="RH222_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HDV3799585.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(1041..1691)
                     /gene="tetR(A)"
                     /locus_tag="RH222_004710"
     CDS             complement(1041..1691)
                     /gene="tetR(A)"
                     /locus_tag="RH222_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HDV3799586.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1797..2996
                     /gene="tet(A)"
                     /locus_tag="RH222_004711"
     CDS             1797..2996
                     /gene="tet(A)"
                     /locus_tag="RH222_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HDV3799587.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(3028..3888)
                     /locus_tag="RH222_004712"
     CDS             complement(3028..3888)
                     /locus_tag="RH222_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV3799588.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
BASE COUNT          759 a         1233 c         1207 g          737 t
ORIGIN      
        1 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
       61 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
      121 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
      181 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
      241 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
      301 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
      361 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
      421 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
      481 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
      541 ccagcccggt gagcgtcgga aaggcgctgg aagccccgta gcgacgcgga gaggggcgag
      601 acaagccaag ggcgcaggct cgatgcgcag cacgacatag ccggttctcg caaggacgag
      661 aatttccctg cggtgcccct caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag
      721 agccttgagt ccacgctaga tctatctcat ctgcgcaagg cagaacgtga agacggccgc
      781 cctggacctc gcccgcgagc gccaggcgca cgaggccggc gcgcggaccc gcgccacggc
      841 ccacgagcgg acgccgcagc aggagcgcca gaaggccgcc agagaggccg agcgcggccg
      901 tgaggcttgg acgctagggc agggcatgaa aaagcccgta gcgggctgct acgggcgtct
      961 gacgcggtgg aaagggggag gggatgttgt ctacatggct ctgctgtagt gagtgggttg
     1021 cgctccggca gcggtcctga tcaatcgtca ccctttctcg gtccttcaac gttcctgaca
     1081 acgagcctcc ttttcgccaa tccatcgaca atcaccgcga gtccctgctc gaacgctgcg
     1141 tccggaccgg cttcgtcgaa ggcgtctatc gcggcccgca acagcggcga gagcggagcc
     1201 tgttcaacgg tgccgccgcg ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg
     1261 gccccaacag tgaagtagct gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa
     1321 cccgcctcgc agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt
     1381 cgcgtgccgg catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg
     1441 cgggcattcc cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta
     1501 tgattctccg ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag
     1561 tgccagtaaa gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga
     1621 ccgtctacgc cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc
     1681 aactttgtca tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag
     1741 tgataaagaa tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga
     1801 aacccaacag acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg
     1861 gcctgattat gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca
     1921 ccgcccacta tggcattctg ctggcgctgt atgcgttgat gcaatttgcc tgcgcacctg
     1981 tgctgggcgc gctgtcggat cgtttcgggc ggcggccggt cttgctcgtc tcgctggccg
     2041 gcgctgctgt cgactacgcc atcatggcga cggcgccttt cctttgggtt ctctatatcg
     2101 ggcggatcgt ggccggcatc accggggcga ctggggcggt agccggcgct tatattgccg
     2161 atatcactga tggcgatgag cgcgcgcggc acttcggctt catgagcgcc tgtttcgggt
     2221 tcgggatggt cgcgggacct gtgctcggtg ggctgatggg cggtttctcc ccccacgctc
     2281 cgttcttcgc cgcggcagcc ttgaacggcc tcaatttcct gacgggctgt ttccttttgc
     2341 cggagtcgca caaaggcgaa cgccggccgt tacgccggga ggctctcaac ccgctcgctt
     2401 cgttccggtg ggcccggggc atgaccgtcg tcgccgccct gatggcggtc ttcttcatca
     2461 tgcaacttgt cggacaggtg ccggccgcgc tttgggtcat tttcggcgag gatcgctttc
     2521 actgggacgc gaccacgatc ggcatttcgc ttgccgcatt tggcattctg cattcactcg
     2581 cccaggcaat gatcaccggc cctgtagccg cccggctcgg cgaaaggcgg gcactcatgc
     2641 tcggaatgat tgccgacggc acaggctaca tcctgcttgc cttcgcgaca cggggatgga
     2701 tggcgttccc gatcatggtc ctgcttgctt cgggtggcat cggaatgccg gcgctgcaag
     2761 caatgttgtc caggcaggtg gatgaggaac gtcaggggca gctgcaaggc tcactggcgg
     2821 cgctcaccag cctgacctcg atcgtcggac ccctcctctt cacggcgatc tatgcggctt
     2881 ctataacaac gtggaacggg tgggcatgga ttgcaggcgc tgccctctac ttgctctgcc
     2941 tgccggcgct gcgtcgcggg ctttggagcg gcgcagggca acgagccgat cgctgatcgt
     3001 ggaaacgata ggcctatgcc atgcgggtca aggcgacttc cggcaagcta tacgcgccct
     3061 aggagtgcgg ttggaacgtt ggcccagcca gatactcccg atcacgagca ggacgccgat
     3121 gatttgaagc gcactcagcg tctgatccaa gaacaaccat cctagcaaca cggcggtccc
     3181 cgggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa
     3241 ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
     3301 ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag
     3361 tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt
     3421 gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc
     3481 gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca
     3541 aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa
     3601 cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta
     3661 gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat
     3721 gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc
     3781 cggcaacgcc cgcagcatcg cgaccgtcat cggtgagaag ttcggcaggt attgggtggt
     3841 gacaatgtag gtgctgcccc aaatggcagg tgctatcgct gtgaacaata aatcggggca
     3901 gacggcgctt ccagcgtgac gaccatgccg agcacg
//
LOCUS       DAPHJE010000378         1233 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16298
            SAMN37211115-rid19347983.guided.301, whole genome shotgun sequence.
ACCESSION   DAPHJE010000378 DAPHJE010000000
VERSION     DAPHJE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211115
            Sequence Read Archive: SRR25824493
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875217.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875217.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:35:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,714
            CDSs (total)                      :: 4,631
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 83
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 51 of 118
            Pseudo Genes (incomplete)         :: 68 of 118
            Pseudo Genes (internal stop)      :: 33 of 118
            Pseudo Genes (multiple problems)  :: 29 of 118
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211115-rid19347983.guided.301"
                     /isolate="ARS-CC16298"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..593
                     /gene="dfrA8"
                     /locus_tag="RH222_004713"
     CDS             84..593
                     /gene="dfrA8"
                     /locus_tag="RH222_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA8"
                     /protein_id="HDV3799589.1"
                     /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVI
                     MGRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDV
                     AYVIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYF
                     TYRKKELTE"
     gene            590..>1233
                     /locus_tag="RH222_004714"
     CDS             590..>1233
                     /locus_tag="RH222_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV3799590.1"
                     /translation="MTFVAISDTHLHNWSQFAIPTESGINSRLLQILKAIEEAACAAD
                     YHAPAGVVPTVYHGGDLFHVRGSLTPSVLNAVLDFFKTIHRDYGVRFRMIAGNHDLET
                     KDSCPMGNAAAALNSLPFVEVVSEKTLFEDHKVALLPWRDSMDDLRADLAHVKDAIGA
                     SVASKWTAIIHAPVNGVVLGIPDHGFDGKELASYGFGLVLAGALLQIVGGDKLI"
BASE COUNT          296 a          323 c          322 g          292 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc cgacgcaata atcaccaatt
       61 cctcttttga taggtaaaca acaatgatcg agcttcatgc cattttagct gccaccgcca
      121 atggttgcat tgggaaggac aacgcacttc cctggccacc actaaaaggc gatctggcca
      181 gattcaaaaa attgaccatg gggaaggtgg tcattatggg gcgcaagacc tatgagagct
      241 tgcccgtcaa attagaaggt cgcacctgca tcgttatgac gcgccaagcg ctggagcttc
      301 cgggtgttcg tgacgctaac ggcgctatct tcgtgaacaa cgtcagcgac gccatgcggt
      361 tcgctcaaga agagagcgtg ggcgatgtgg cctacgtcat tggtggcgct gagatattca
      421 agcgacttgc cttgatgatc acgcagattg aattgacctt tgttaagcga ctgtacgaag
      481 gcgacaccta cgttgatctg gccgaaatgg tcaaagacta cgagcagaat ggcatggaag
      541 aacatgacct tcacacttac ttcacttacc gtaaaaagga gcttacagaa tgaccttcgt
      601 tgccatttca gatacgcacc tgcacaactg gtcgcagttc gcgataccaa cagagtcggg
      661 cattaactcc aggctgttgc agattcttaa agccattgaa gaagcggctt gcgccgccga
      721 ttaccacgcc ccagcgggcg tggttccaac cgtctatcac ggcggcgatc tcttccatgt
      781 gcgaggttct ttgacccctt ctgtgctaaa cgcggtgctc gactttttta agaccatcca
      841 ccgtgactac ggcgttcgct ttcgcatgat cgccggcaat cacgatctgg aaaccaaaga
      901 ctcatgtcct atgggcaatg cagctgcggc gcttaattcg ttgccgtttg tggaggttgt
      961 atccgaaaaa acgctgttcg aggaccacaa agtcgctctg ctgccatggc gcgattccat
     1021 ggacgatctg cgtgcggatc ttgcgcacgt taaagacgcg atcggcgcta gtgttgcctc
     1081 taagtggaca gcaatcatcc acgcaccggt taacggggtt gttcttggca ttccagacca
     1141 tgggttcgac ggaaaagagc tcgcctccta tggcttcggg ttagtgctcg ccggggcact
     1201 gttgcaaata gtcggtggtg ataaacttat cat
//
